LOCUS       DAMJVX010000001        83328 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMJVX010000001 DAMJVX010000000
VERSION     DAMJVX010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 83328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83328
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.001"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            33..1100
                     /locus_tag="PC407_000001"
     CDS             33..1100
                     /locus_tag="PC407_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HDF2495488.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSIEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSSSRNNYQPKVL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            1270..3171
                     /gene="cooS"
                     /locus_tag="PC407_000002"
     CDS             1270..3171
                     /gene="cooS"
                     /locus_tag="PC407_000002"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2495489.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            3164..3637
                     /locus_tag="PC407_000003"
     CDS             3164..3637
                     /locus_tag="PC407_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2495490.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            3612..4838
                     /locus_tag="PC407_000004"
     CDS             3612..4838
                     /locus_tag="PC407_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2495491.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDVCY
                     ADFFNIKENGEFCYSV"
     gene            complement(4977..6164)
                     /locus_tag="PC407_000005"
     CDS             complement(4977..6164)
                     /locus_tag="PC407_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2495492.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKSFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            6437..7261
                     /locus_tag="PC407_000006"
     CDS             6437..7261
                     /locus_tag="PC407_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF2495493.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            7390..8655
                     /gene="gluD"
                     /locus_tag="PC407_000007"
     CDS             7390..8655
                     /gene="gluD"
                     /locus_tag="PC407_000007"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HDF2495494.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            8848..9504
                     /locus_tag="PC407_000008"
     CDS             8848..9504
                     /locus_tag="PC407_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2495495.1"
                     /translation="MYFLPPHDYKLEELDWIFGHELMYFKSKDLHLKYLVLFLKIVYW
                     FNPFIYIMEKYMDLDCELYCDERVLKNCTVQQKQEYALTIVNSMKRGINFSNKFVTGL
                     NKQSDIRKRVMNMFSEKHKNGILMALIISLFSSITFFKFDSTSLIEENDFLERSRYAF
                     EGRQEDMFVTIDSTYADAPIKLRKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK
                     "
     gene            9835..10968
                     /locus_tag="PC407_000009"
     CDS             9835..10968
                     /locus_tag="PC407_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HDF2495496.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGF
                     TYADAPKNMREEYKINCESLGLIPKDSDKIEVGVEYYDSLLND"
     gene            11895..12917
                     /locus_tag="PC407_000010"
     CDS             11895..12917
                     /locus_tag="PC407_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2495497.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            13213..14133
                     /gene="pyrB"
                     /locus_tag="PC407_000011"
     CDS             13213..14133
                     /gene="pyrB"
                     /locus_tag="PC407_000011"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDF2495498.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            14172..14864
                     /locus_tag="PC407_000012"
     CDS             14172..14864
                     /locus_tag="PC407_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDF2495499.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            14857..15759
                     /locus_tag="PC407_000013"
     CDS             14857..15759
                     /locus_tag="PC407_000013"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2495500.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            15844..16428
                     /locus_tag="PC407_000014"
     CDS             15844..16428
                     /locus_tag="PC407_000014"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDF2495501.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            16643..17569
                     /locus_tag="PC407_000015"
     CDS             16643..17569
                     /locus_tag="PC407_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HDF2495502.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFNYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIGKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYILSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVENSLHRILEHSKEGRTSMLQDVEAHRPTEVDMF
                     SKNICKLGKKYSIPTPINQTLFYMIKVIESRF"
     gene            complement(17621..18511)
                     /locus_tag="PC407_000016"
     CDS             complement(17621..18511)
                     /locus_tag="PC407_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2495503.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSQKYPDIYVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYNSHLLKDFSFDKLQDQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            18699..19730
                     /locus_tag="PC407_000017"
     CDS             18699..19730
                     /locus_tag="PC407_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDF2495504.1"
                     /translation="MLKKKSSLTLKTIREILPGLFISVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGAIVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
     gene            20024..20899
                     /locus_tag="PC407_000018"
     CDS             20024..20899
                     /locus_tag="PC407_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HDF2495505.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     RGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            20917..22407
                     /gene="cls"
                     /locus_tag="PC407_000019"
     CDS             20917..22407
                     /gene="cls"
                     /locus_tag="PC407_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2495506.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            22600..22884
                     /locus_tag="PC407_000020"
     CDS             22600..22884
                     /locus_tag="PC407_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDF2495507.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            22917..24545
                     /gene="groL"
                     /locus_tag="PC407_000021"
     CDS             22917..24545
                     /gene="groL"
                     /locus_tag="PC407_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDF2495508.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVIVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            24807..25547
                     /gene="larB"
                     /locus_tag="PC407_000022"
     CDS             24807..25547
                     /gene="larB"
                     /locus_tag="PC407_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HDF2495509.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            25631..26365
                     /gene="larC"
                     /locus_tag="PC407_000023"
     CDS             25631..26365
                     /gene="larC"
                     /locus_tag="PC407_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HDF2495510.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            26395..26814
                     /locus_tag="PC407_000024"
     CDS             26395..26814
                     /locus_tag="PC407_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HDF2495511.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKVTP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      26874..26975
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            27196..28731
                     /gene="guaA"
                     /locus_tag="PC407_000025"
     CDS             27196..28731
                     /gene="guaA"
                     /locus_tag="PC407_000025"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDF2495512.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTSITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            29270..30700
                     /locus_tag="PC407_000026"
     CDS             29270..30700
                     /locus_tag="PC407_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2495513.1"
                     /translation="MILNQIFIEDGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPRNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGEYISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKNNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(30848..31723)
                     /locus_tag="PC407_000027"
     CDS             complement(30848..31723)
                     /locus_tag="PC407_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2495514.1"
                     /translation="MINIRYLEILEAIEQTGTFTGAAKKLHITQSAVSHAIAELEQQA
                     ETSLFDRLPRGVCLTRCGISLLDEARSILTACRNLDKKISRLEECTPINIVSSITIAS
                     FLLPPILSRLKNSFPSLKVNVRVESANSAMDILKSGEADICFWEGIEPQGAFRTILLG
                     SYKLCVACAPTFPLSEQTLSLRQLCTFPLLLREQGSAIRDTLDSVLSLSNQKAYPVWE
                     SVNSFALIKAAEAGLGITILPENLLLDSLLHKKLRLIELDEIDMENKMLAILHKDKNI
                     TQPLEIILDNISNVL"
     gene            31843..32406
                     /locus_tag="PC407_000028"
     CDS             31843..32406
                     /locus_tag="PC407_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2495515.1"
                     /translation="MNQSRKIYFWLLIINLFISTFTFGGGYVVVPMIRRYFVNQKQFF
                     TEDDLMSMAAVAQSTPGAIAINLSALAGYRVAGTLGVVISCTAAIIPPLVILGLVSAF
                     YTTFISNAIVTAVLKGMQAGVAALIVDLIIDMCSMILKQRSLFLSTMIPIAFFANFVM
                     SINVIFILSVCCFVCVMQVFYKKRENK"
     gene            32403..32969
                     /locus_tag="PC407_000029"
     CDS             32403..32969
                     /locus_tag="PC407_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2495516.1"
                     /translation="MNTIWNLFFIFLKIGLFSIGGGYAIIPLIQEKVVNQTGWISEKM
                     FTDIITISQMTPGPLAVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKH
                     QNSTYIFEILNGLKSASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFV
                     ILLIVRKWKINPIVIIIISGILGIFFYL"
     gene            complement(33098..33334)
                     /locus_tag="PC407_000030"
     CDS             complement(33098..33334)
                     /locus_tag="PC407_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495517.1"
                     /translation="MLECFIAEDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(33644..37072)
                     /gene="uvrA"
                     /locus_tag="PC407_000031"
     CDS             complement(33644..37072)
                     /gene="uvrA"
                     /locus_tag="PC407_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2495518.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFENELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFAKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLGNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKTPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPNAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPNKNYTALTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFTETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCNEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            37391..39625
                     /locus_tag="PC407_000032"
     CDS             37391..39625
                     /locus_tag="PC407_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2495519.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMDEILNIDSFNGEGTIEIINSQGKTLFIGKNNELIKDLSYKYKDSADEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDVVLGELNK
                     LNSFTIIMTFITTCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSNFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            39881..41911
                     /locus_tag="PC407_000033"
     CDS             39881..41911
                     /locus_tag="PC407_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2495520.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDLIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENEIILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDEDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            41908..42360
                     /locus_tag="PC407_000034"
     CDS             41908..42360
                     /locus_tag="PC407_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2495521.1"
                     /translation="MNKLVNVKCSFKIHANSKEEAIVSLVDVVSKEGYLIDKNQFLED
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDIPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            42362..43417
                     /locus_tag="PC407_000035"
     CDS             42362..43417
                     /locus_tag="PC407_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDF2495522.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            43484..43792
                     /locus_tag="PC407_000036"
     CDS             43484..43792
                     /locus_tag="PC407_000036"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDF2495523.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            43896..45161
                     /locus_tag="PC407_000037"
     CDS             43896..45161
                     /locus_tag="PC407_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HDF2495524.1"
                     /translation="MKKLPIKTVVQRLLDLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKIADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLSVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVDKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKNEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            45249..45965
                     /locus_tag="PC407_000038"
     CDS             45249..45965
                     /locus_tag="PC407_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2495525.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(46160..46453)
                     /locus_tag="PC407_000039"
     CDS             complement(46160..46453)
                     /locus_tag="PC407_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HDF2495526.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(46459..46674)
                     /locus_tag="PC407_000040"
     CDS             complement(46459..46674)
                     /locus_tag="PC407_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495527.1"
                     /translation="MRTLSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            complement(47219..47731)
                     /locus_tag="PC407_000041"
     CDS             complement(47219..47731)
                     /locus_tag="PC407_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2495528.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKELSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVKQILDLPVKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            48181..48657
                     /gene="purE"
                     /locus_tag="PC407_000042"
     CDS             48181..48657
                     /gene="purE"
                     /locus_tag="PC407_000042"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDF2495529.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            48663..49361
                     /locus_tag="PC407_000043"
     CDS             48663..49361
                     /locus_tag="PC407_000043"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2495530.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            49373..50740
                     /gene="purF"
                     /locus_tag="PC407_000044"
     CDS             49373..50740
                     /gene="purF"
                     /locus_tag="PC407_000044"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDF2495531.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            50734..51810
                     /locus_tag="PC407_000045"
     CDS             50734..51810
                     /locus_tag="PC407_000045"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDF2495532.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            51804..52397
                     /locus_tag="PC407_000046"
     CDS             51804..52397
                     /locus_tag="PC407_000046"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDF2495533.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            52390..53922
                     /gene="purH"
                     /locus_tag="PC407_000047"
     CDS             52390..53922
                     /gene="purH"
                     /locus_tag="PC407_000047"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDF2495534.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEVLKQKKNLRILKLAKIEDSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKAKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            53933..55183
                     /gene="purD"
                     /locus_tag="PC407_000048"
     CDS             53933..55183
                     /gene="purD"
                     /locus_tag="PC407_000048"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDF2495535.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKVYCAPGNAGITNVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMETILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMLDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            55208..59014
                     /locus_tag="PC407_000049"
     CDS             55208..59014
                     /locus_tag="PC407_000049"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDF2495536.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAVPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKTFTDDKLFE
                     ASYGNIVLELANDDLCMLEGYNYVVLGSTTKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERVGA
                     NANIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVFKNIIGEKDQKIFES
                     GVKYFR"
     gene            59220..60092
                     /gene="rfbD"
                     /locus_tag="PC407_000050"
     CDS             59220..60092
                     /gene="rfbD"
                     /locus_tag="PC407_000050"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDF2495537.1"
                     /translation="MKILITGSNGQLGKELVSQLEAINQSINQPKYVILATTRNDLDI
                     SNQTNIDNFILHNKPDVIVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LVHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFELKNIDIKVNPIKSNEFKCKAQRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            60201..61082
                     /gene="rfbA"
                     /locus_tag="PC407_000051"
     CDS             60201..61082
                     /gene="rfbA"
                     /locus_tag="PC407_000051"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDF2495538.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLKITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDMQYGR"
     gene            61119..61676
                     /gene="rfbC"
                     /locus_tag="PC407_000052"
     CDS             61119..61676
                     /gene="rfbC"
                     /locus_tag="PC407_000052"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDF2495539.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFCDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVRYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFNYKCTNYYYPEFEHGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICIRR"
     gene            61714..62697
                     /gene="rfbB"
                     /locus_tag="PC407_000053"
     CDS             61714..62697
                     /gene="rfbB"
                     /locus_tag="PC407_000053"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDF2495540.1"
                     /translation="MKKILITGGAGFIGSNFVHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDSSNYKFVKGDISNREQMYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSPINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLDKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            62736..63404
                     /locus_tag="PC407_000054"
     CDS             62736..63404
                     /locus_tag="PC407_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2495541.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNSSSGFDMIMMTLLK
                     ALSQNNQQTSNGYNLSSNSENLFDELGSVVNNTASRFIDVDTKDKSVKSRIENAIEQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            63413..63841
                     /locus_tag="PC407_000055"
     CDS             63413..63841
                     /locus_tag="PC407_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HDF2495542.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            63865..64179
                     /locus_tag="PC407_000056"
     CDS             63865..64179
                     /locus_tag="PC407_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2495543.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            64331..64606
                     /gene="flgM"
                     /locus_tag="PC407_000057"
     CDS             64331..64606
                     /gene="flgM"
                     /locus_tag="PC407_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HDF2495544.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGVKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            64611..65027
                     /locus_tag="PC407_000058"
     CDS             64611..65027
                     /locus_tag="PC407_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HDF2495545.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDRYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTATYNSYGNIRK"
     gene            65045..66355
                     /gene="flgK"
                     /locus_tag="PC407_000059"
     CDS             65045..66355
                     /gene="flgK"
                     /locus_tag="PC407_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDF2495546.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGNTVSKYDYY
                     NTIETIINEPSDYGISAGIDDFFKGWESLAKDINSDSKKSLVVENASNLATLISESYK
                     KLNKLKGDIDDNINSEIKQINDMLKSLEDLNKSIDVISGSGSTPNDLLDERDRILDDL
                     SFKLDLENSDVKNMLSDGKLELNELKNADGTWKTGVSGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKVNPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESVGLDSQKVNQDEVNQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            66374..67306
                     /gene="flgL"
                     /locus_tag="PC407_000060"
     CDS             66374..67306
                     /gene="flgL"
                     /locus_tag="PC407_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDF2495547.1"
                     /translation="MRVSTGMMSSNYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            67322..67714
                     /locus_tag="PC407_000061"
     CDS             67322..67714
                     /locus_tag="PC407_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HDF2495548.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDSLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            67708..67920
                     /gene="csrA"
                     /locus_tag="PC407_000062"
     CDS             67708..67920
                     /gene="csrA"
                     /locus_tag="PC407_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDF2495549.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            67942..68337
                     /gene="fliS"
                     /locus_tag="PC407_000063"
     CDS             67942..68337
                     /gene="fliS"
                     /locus_tag="PC407_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF2495550.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            68337..68693
                     /gene="fliS"
                     /locus_tag="PC407_000064"
     CDS             68337..68693
                     /gene="fliS"
                     /locus_tag="PC407_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF2495551.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELIRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            68714..70237
                     /gene="fliD"
                     /locus_tag="PC407_000065"
     CDS             68714..70237
                     /gene="fliD"
                     /locus_tag="PC407_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDF2495552.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLKVIGNDNLAQQIANDNGIT
                     FANDASGNKVASVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            70240..70575
                     /gene="fliT"
                     /locus_tag="PC407_000066"
     CDS             70240..70575
                     /gene="fliT"
                     /locus_tag="PC407_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDF2495553.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISSKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            70677..71549
                     /locus_tag="PC407_000067"
     CDS             70677..71549
                     /locus_tag="PC407_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HDF2495554.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTTN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTDAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            71642..73783
                     /locus_tag="PC407_000068"
     CDS             71642..73783
                     /locus_tag="PC407_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2495555.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRSSIESELIIVDTGSTDD
                     TINISRKYTENVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNIDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFEELKENDNSPYTNYQLGKNFIILGKYHEAL
                     YYLEKSYELYSKLESAPGYLITSLVKTYLYLGKHKKCEKLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYESVISEYNNIEDMEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YKNIEEVINNIKDDEKEHVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKVAFEDKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDI
                     KKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYRNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNDANEKLEYIHSMRKLLNEYPFYNKIIKILISELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKSLVDDYSKTFMDDVKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            73807..75684
                     /locus_tag="PC407_000069"
     CDS             73807..75684
                     /locus_tag="PC407_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2495556.1"
                     /translation="MNTPIVVNEKSKDIIKDWQYTYTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFMFAHKINTSNINLWKYRLKLVGFCMLIKKSVLDNVGVLDELFTPGNFEDDDLSFR
                     MLESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIADIEKVKLDYEKDFFDYIILGDVLEHLTDPWKIVNELKQYLKKDGCLISSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSVTATSMPLSEID
                     NRFIDTMCKESCKEMRQQYESYQYIVKATKSIDTKRYSSISMVNLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLVDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSKKVQSESDIQDILYFIEGKRY
                     E"
     gene            75677..77791
                     /locus_tag="PC407_000070"
     CDS             75677..77791
                     /locus_tag="PC407_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2495557.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDAFIINKNFIYDILEIFKKNTDVGMIGVAGVKDI
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVANINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLDIVSKIAHFS
                     VFNHTGLYSCEKCERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAIDDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKEKQSEIKHFVIDKHCIEIKNYIDKIYKEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            77800..78795
                     /locus_tag="PC407_000071"
     CDS             77800..78795
                     /locus_tag="PC407_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HDF2495558.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIIYKGEEILAVVPACETYENGERVFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLETYLQEQSFNKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKK
                     DILANFRYSKRRYFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            78887..80119
                     /locus_tag="PC407_000072"
     CDS             78887..80119
                     /locus_tag="PC407_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDF2495559.1"
                     /translation="MKRLMILGSIKYFENIVMSAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEIFDENNENMIENF"
     gene            80139..81083
                     /locus_tag="PC407_000073"
     CDS             80139..81083
                     /locus_tag="PC407_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HDF2495560.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSDIFLYNMKDAKLKAILDGNYITA
                     FRTAAVAGLAIDTFAKKDFNTISIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFIKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYNVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLNCEEIELKEPKEKYWI"
     gene            81177..81584
                     /locus_tag="PC407_000074"
     CDS             81177..81584
                     /locus_tag="PC407_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HDF2495561.1"
                     /translation="MDKYNLIKFLSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKESIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNM"
     gene            81671..82762
                     /locus_tag="PC407_000075"
     CDS             81671..82762
                     /locus_tag="PC407_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HDF2495562.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVRHFEEE
                     FASYVGSKYCVGLASGLDALIIAFRALGISEGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASKMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKNGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFVGMEDEEVSYIIKIINEY"
     regulatory      83071..83161
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT        32885 a         8784 c        14865 g        26794 t
ORIGIN      
        1 tttaaaattt attaatacaa aggagattta ctatgaaaaa taaattagtt tacatatgta
       61 ttatgagtct attgctattt gtagtaggat gttctaataa tccaaataag gaaagtacca
      121 ttaaaaaaga ctctaagaat aaccaaagcc aagttaaaga agaaaaaaga gaacctgtga
      181 aaaaagatat aactataagt ttcgctggtg atgtaactat ggggaactat aaaggttcat
      241 catattatgg cagttttgac catgaattta aaagacaagg agaagattac aattattttc
      301 ttaaaaatgt aaaacctgta tttgaaaaag atgacttaac agtggttaat ttagaaggtc
      361 cacttactac agcgtcaaat gcaaaagaaa aaaaatttgc atttaaagga aagcctggat
      421 atgtaaatat tttaaaatct ggaagtatag aggcagtaag tattgcaaat aatcattcag
      481 aagattattt tgaagaagga atgaaagata cacagtttat attggatgag aataaaataa
      541 attattttgg attagggaaa gatgctgttg tagatgtcaa aggaattaaa gtgggtctat
      601 tagggtataa tggattatca actgaatata atgaaaaaaa tttaaaacaa atggaagatg
      661 atattaagtc cttaaaaaag aaatcggatg ttgttgtagt gtactttcat tgggggatag
      721 aattagatta ttatccagat aaaaaacaaa aagattttgc tcattatgct atagacaagg
      781 gagcagattt agttatgggg tctcacccac atgttattca aggaatagaa aagtataaaa
      841 ataagtatat agcttatagt ctagggaatt tttgctttgg aggaaataag aatccttctg
      901 atacagatag ttatatatat caacaaacct ttactttttc agataataaa ttaacttcaa
      961 ttaaagagcc aaatataata ccaacctcaa taacatctag tagctccaga aataattatc
     1021 aaccaaaagt attggatgga agtgagaagg atagagtttt aaataaactt gaaaaaataa
     1081 gcaaagattt aaataaataa aatgtaaaaa aaaataaata tatatattta ttttaataat
     1141 ttatattttg tttagggtat ggtaaatcgt ggtattaaat aagtagattt taatagttaa
     1201 attatagtca attataataa agctatttaa tctacttttt taatataaaa atataagggg
     1261 gaatttgtaa tggcttgtaa cacttgtaaa atgtgcgaaa gtgcagacaa gaaattagaa
     1321 agttttgtag cttcaaagga tgttgaaaca gcatttcata gaacagaaga tcagaaagtt
     1381 aagtgtggtt ttggacttca gggggtttgt tgtagactct gttcaaatgg accatgtaga
     1441 gtaactccta aatcaccaag aggtatatgt ggagcagatg cagacactat agtagcaaga
     1501 aactttttaa gagcagtagc atcaggagca gcttgttatt tacatgtggt agaaaataca
     1561 gctaagaatt taaaaaatgt tggaataact aaaggtgttg ttaaaggaga aaaaacatta
     1621 aatcaattag cagagatgtt tggaatagaa agtgatgaaa agtatgataa atgtataaag
     1681 gttgcagaca aagttatcaa cgatttgtat aaatcaagag atgataaaat ggaattagta
     1741 gaaaaaatag cttatgcacc tagagtgaaa aagtggaaag agttaggtat aatgcctggt
     1801 ggtgcaaagt cagaagtttt tgatgcaata gtaaaatcat caacaaattt aaatagtgat
     1861 ccagtagaca tgctagttaa ttgtttgaac ttaggtatat caacaggatt atatggtctt
     1921 acactaacta atcttttaaa tgatgttatg ttgggagaac cagttataag aatggctcct
     1981 gtaggattta atgtaataga cccagactac ataaatataa tgataacagg ccatcaacat
     2041 tcaacatttg caaatttcca agatagatta aaagatgaag atgttataaa attagctcaa
     2101 tctgttggtg caaaaggctt taagctagta ggatgtacat gtgttggtca agatttacaa
     2161 ttaagaggag aacattatca agaagtattt gctggacatg caggtaacaa ttttacaagt
     2221 gaggctgttt tatcaactgg agcaatagat atagttcttt cagaatttaa ctgtactata
     2281 ccagggcttg aaccaatagc agataaatat aaagtaaaaa tggtttgttt agatgatgtt
     2341 gctaaaaaag cgaatgcaga atacataggt ttagacagaa gtaaattaga tgaacttagt
     2401 aacactttaa tagaaaaagc attggagtct tataaggaga gaagaggaag tattgagata
     2461 gatataccaa aagaccatgg atttgaacaa tctttaactg gtgtaagtga aaagaacctg
     2521 aaagcgtttt tgggagattc atggaaacct cttattaatt taattgcaga aggaaaaatt
     2581 aaaggtgttg cagctgttgt aggatgttca aatatgacag caggaggaca tgatgtaaat
     2641 actgttgaac ttactaaaga attgattaaa aaagatataa tagtactatc tgcaggatgt
     2701 tctactggag gattagaaaa tgttggtctt atgtcacctg gagcagaaga attagcagga
     2761 gaaaacttaa aagaagtatg taagacttta ggaataccac cagtattaaa ctttggacca
     2821 tgtctagcta taggaagact agaaatagta gcaacagaat tagcagctga acttggaatt
     2881 gacctaccac aattaccatt agtgctttca gctccacaat ggttggaaga acaagcatta
     2941 gctgatggag cgtttggtct tgcccttggc ttacctcttc atttagcttt acctccattt
     3001 ataacaggag gaaaattggt gacagaagta ttaacagaaa aattaaaaga cttaacagga
     3061 ggacatgtta tagtgaatcc agaccctaaa tcttcagcta atcaattaga agaaataata
     3121 atagatagaa gaaaaaattt agggttaaag gatgtggaat gcaatgcata gaatttttat
     3181 aaataaagat ttatgtacag gatgtaagag ctgtgtacta gcatgtatgc taaaacacaa
     3241 taaagattat gacatgtaca ctcttgattt agaaaatata gataatgata gtagagggca
     3301 tatagaatta gatagtaaac ataataatcc agttccaatt ctttgtagac actgtgatga
     3361 acctgaatgt gtattagcat gtatgagtgg tgcaatgcat aaagatagtg aaagtggaat
     3421 cgtttcatat gatgaagaga aatgtggttc atgttatatg tgtgtaatgt cttgtccata
     3481 tggattatta aagccagatg atagaagcaa gcaaaacata cttaaatgtg atttatgtaa
     3541 agatgaggag tatcctagat gtgtagctaa ttgcccaagt ggagcaattg aattacagaa
     3601 ggaggaaaat gatgaattat gtagtgttag gggctagtgc agctggtata aatgcagtaa
     3661 aaacattaag agaattagat aaagattcaa atatagtagt aatttctaaa gatgaaaacg
     3721 tatattcaag atgtatgttg catcacgtta tatctgaaca cagaacttta aaacaaataa
     3781 attttgttga tgaagatttt atggagcaaa ataatgtaaa atggattgca ggtaagactg
     3841 ttaaaggtat agacataaat aaaaaggtag ttcaaacaga agacattact gtaaattatg
     3901 ataaactttt aatagctaca ggtgcatctt ctgctattcc tcctataaaa aatttaagag
     3961 atggaaattt tgtatattct gtgagaaaca tagatgatat ttataaaata aaagaaaaag
     4021 cacaaaattc taaaaatgta gttataatag gtgctggttt agttggtata gatgcattag
     4081 tgggattatt taaatatgag cagttaaata tttctgtagc ttttatggaa aagtacattt
     4141 tagatagaca attggatgaa tatacagcat cagtgtatga aaataagttc aaagaaaaag
     4201 gtgtaaaatt ttacccaagt gcatcaatac aagaaattgt attggataac agtaaaaatg
     4261 ttacaggtgt tgcattttca aatggtgaag ttttagatgc agacatggta attgtagcta
     4321 caggggttaa accaaatgct gactttttag atgggacagg aatagaatat gatagaggta
     4381 taataataga tgatatgtgc caaacaacac aaaaagacat atatgcagca ggagatgttg
     4441 taggtaaaaa tgctatttgg cctctggcag taaagcaagg aattgtagct gcctataata
     4501 tggttggaaa agataagaag atagaagatg aatttgcttt taaaaatagt atgaatttca
     4561 tggatatacc aactatttct ataggaatga atacacctgt agatgatagc tataaagtat
     4621 taaccagaca tggaattaat gattataaga agtttgtttt taaagataat gtaatctacg
     4681 gagcagttat acaaggagat atttcatatg taggtgtatt aacttattta ataaaaaata
     4741 aagttgaaat atatgattta gaaaatagga tatttgatgt atgttatgca gactttttta
     4801 atataaaaga aaatggagaa ttttgttata gtgtatagta tatacgtgga gtaaggttta
     4861 taaatacagt aaatcaataa atagagttat tctaaaataa gaaaaatgac aaatcttatt
     4921 tagaaatagc tctatttttt gtaattaaat ttaaattata ttcaatttgt aaaatattat
     4981 tttgaacaat tatacatata ctctctagtt agaggtaaat cattttttat acctttagtg
     5041 aaaagtattt gatgtatatc tataatacca gtgttgaaag cagcagcaca agaacaaagg
     5101 tataaatccc acattcttat aaacttatca tcaaacattt ctttaatctt tggaagattc
     5161 tcagagaaac ttttttgcca gtatagtaag gtttttacat aatgtaatct taaactttct
     5221 acgtctatag tatgatattt gtaatcacta caaatatgta tcatttctct taggctagga
     5281 ataactcctc ctgggaaaat atattttttt atccaagggt ctccataaga ttcctgcaat
     5341 ccacttatat agtgaagtaa aaacaatcca ccatctttta aaacagcatt tacgttttta
     5401 atgaaaagtt catagttttc tctaccaaca tgttcaagca ttccaacgct tacaactcta
     5461 tcaaacataa ggtctgattt ttttaattct ctatagtcca taagtcttac ctcaagatag
     5521 tcttgaaggt tctcttcttt aattcttgcg ttaaattcct taaattgctc ttcgctaagt
     5581 gtaattccaa cgccgtgaat tttatatttt tttgcagctt ctattagtaa aaatccccaa
     5641 ccacaaccaa tatcgagaag gctcatacct tcctctaaat ttagtttagt taatatgtgg
     5701 tgaactttat tgacttgagc atcataaaga gtatcattat catttctaaa gtatgcacaa
     5761 gaataactaa gtgtcttatc taaccataag ctataaaaat cgttgccaat gtcatagtgg
     5821 gcatgtattt ctttcttctg atgtctcatt gaatttgatg agtgcaatag atgttttaca
     5881 gctgaattat caatattgaa tttgtctatt tgttttaaaa gtagatttaa agttaagaat
     5941 aaatctccct caacttcaat gtctctattt atgtaggctt ctccaagtgc taaagatgta
     6001 ctttttaata gttcagactt gctaatatca ttatttacta taactttgaa ctgaggggag
     6061 ccctctccaa ttagaaattc ttcctcatct ttaagcctaa ctagaaatgg tgtttcacca
     6121 aatttctcta aatagtcttt taaaaaattt tttgtaatat tcatattacc agctccttct
     6181 aatgtaaatc cagtaagttt ccctgcttaa aatatattat agtccttttt ttaaaaaagt
     6241 aaaatttttt caataaattt tttatttaaa ggaaatttat tttttataaa gaaatttcat
     6301 gttgtagtat attttttgct tataaatttt ggtactggat ataatattaa actgttaatg
     6361 atataataaa tttaatctat aaagtaaata aaattttgta ataaatagtt aaagctattt
     6421 tataaggaga tatatgatgg aagtatatgc tttggttggg tcaagtggca cagggaagag
     6481 ctttaaggct ttagagttcg catatgaaaa tgatatagaa tatataatcg atgatgggat
     6541 tttaatatac aaaaacaagg ttttagctgg gatatcggca aaacaagcaa atactactat
     6601 agaagcagtt aaaagggcta ttttttataa tctggagcat agacaagaag ttagagaagc
     6661 aattaaaaaa gaaaatataa gaaaaattct tattattggg acatcaaaaa aaatggttaa
     6721 tcaaattgta gagagattgt caattggaag ggtctgtaag tttataaata tacaagatat
     6781 aagtactaaa tatgaaatag aaatagcaaa acaagctaga aaagaaggaa atcatgtaat
     6841 tcctgttcca gccgttgaga taaaaagtat ggcaagtggt ctcagtataa attctttgaa
     6901 gagacttttt agaaaaggaa ataataggaa tatggtagtt ttagaaaaaa caattataag
     6961 accaactttt agttatatag gaaagtttta tattagtgct gatgtaatta aacagattat
     7021 tgaatatgaa gtatacaact ttggaagcat tgatagaata aataaaatca atatagagaa
     7081 ttgcaataat tttatgaata tttttgtaag tataaatatc aatgatttag aaactataaa
     7141 aaaaattgaa gacatacaac agacaataaa aaagtctatt gagaaaatga ctttagttaa
     7201 tgtacaaaaa atagatatta acattcataa aattaaaaaa ataaacttcc tccaatgtta
     7261 aaaaataaac tgagaaaatg atatactaat ttgcaataaa gtgtgctata attaaactgt
     7321 aaatttagga aatggttttt tctaaatgga gtatacataa ttataacgta tttataaggg
     7381 ggctttcaaa tgtcaggaaa agatgtaaat gtcttcgaga tggcgcaatc tcaagtaaaa
     7441 aatgcatgtg ataaattagg tatggaacca gcagtttatg aattattaaa agaacctatg
     7501 agagttatag aggtttcaat tccagtaaaa atggatgatg gttctataaa aacttttaaa
     7561 ggatttagat cacaacataa tgatgcagta gggccaacaa aaggtggaat aagattccat
     7621 caaaatgttt caagagacga agtaaaagct ttatctatat ggatgacttt caagtgttct
     7681 gtaacaggta taccatatgg tggaggtaaa ggtggaataa tagtagatcc atcaacttta
     7741 tctcaaggtg aattagaaag attaagtaga ggatacatag atggaatata taaattaata
     7801 ggtgaaaaag ttgacgttcc tgcaccagac gtaaatacta atggtcaaat aatgtcttgg
     7861 atggttgatg agtacaataa attaactgga caaagttcta taggtgttat aactggtaag
     7921 ccagttgaat ttggtggttc tttaggaaga acagctgcta ctggatttgg tgttgctgtt
     7981 actgcaagag aagctgctgc taaattagga atagacatga aaaaagctaa aatagctgtt
     8041 caaggtatag gaaacgttgg ttcttataca gttcttaact gtgaaaaact tggtggtact
     8101 gttgtagcta tggctgaatg gtgtaaatca gaaggttctt atgctatata caatgaaaat
     8161 ggtttagatg gtcaagctat gttagattat atgaaagaac atggtaactt attaaacttc
     8221 ccaggagcta agagaatatc tttagaagag ttctgggctt cagatgttga tatagtaata
     8281 ccagctgcat tagaaaactc tataactaaa gaagttgctg aatctataaa agctaaatta
     8341 gtttgtgagg ctgctaatgg accaactact ccagaggctg acgaagtatt tgctgaaaga
     8401 ggaatagttc ttactccaga tatattaact aatgctggtg gagttacagt ttcttacttc
     8461 gagtgggtac aaaacttata tggatactac tggtcagaag aagaagtaga gcaaaaagaa
     8521 gaaatagcta tggttaaagc atttgaatct atttggaaaa tcaaagaaga gtacaatgtt
     8581 acaatgagag aagctgctta catgcattca attaagaaag ttgctgaagc tatgaaatta
     8641 agaggatggt actaagattt actcttaata aataatagga tataattatt taagatataa
     8701 aggggctgta catagtacag cccttaaatt atgtggctta caatgaagta agctattttt
     8761 gttttaactt ataaataact tgataaatta tgtaatttag aatgcataaa taagatgggt
     8821 gaaaattaat ggttgattta attttgaatg tattttttac caccacatga ttacaaatta
     8881 gaggagttag attggatttt tggacatgaa cttatgtatt ttaaaagcaa agaccttcat
     8941 ttaaagtatc tagtattatt tttaaaaata gtatattggt ttaatccatt tatatatata
     9001 atggaaaaat atatggattt ggattgtgaa ctttattgtg atgagagagt tttaaaaaat
     9061 tgtactgtac aacaaaagca agaatatgct ctaactattg taaattcaat gaaaagaggt
     9121 ataaattttt caaataaatt tgtaactgga ttaaataaac aatctgatat tagaaaaaga
     9181 gtaatgaaca tgttcagtga aaaacataaa aatggtatct taatggcttt gattataagc
     9241 ttgttttctt ctattacatt ttttaaattc gattcaacta gtcttattga agagaatgat
     9301 ttcttagaga ggtcaagata tgcgtttgaa ggaaggcaag aagacatgtt tgtaacaata
     9361 gattctacat atgcagatgc acctataaaa cttagaaaag agcatgaaga aaactgtaga
     9421 gtcaatggaa tagtaccaaa agattcagat tcaatagaaa ttattcctag attttataaa
     9481 gattgtatgg atttaaaaaa ataagttaga cttaatattg atgttgatat tatgaatttt
     9541 ttatatttaa gcaattgaaa attttattat agattcaatt ttatatgtgt ttaagtatga
     9601 aatttataga tagttgaaat aattaaatat gattgatgca taggaatcat tactaagcta
     9661 taattaatta taggggagtt taaataaatt atgagttaca caattataaa atgataaaaa
     9721 atagttatct gttacaaaaa atgtatcttt ttaattaata aagataatat gaaaacttaa
     9781 aaggcaaata aacttaaaca taaaatactc ttaaataaga taggagaaaa atgtatgtct
     9841 aactttattc taaatgggat ttttagaaca gttatagtaa gtagtctaag cattcttctg
     9901 atattgattt tcaagaaaaa tgtatttaaa agatttagca aaaaatttaa ttattatata
     9961 tggatgatag ttgttattaa attattttta ccatttacat attatacttt tacaataaat
    10021 atcttgagaa gtaaaaaaca cattaatatt aataacataa atttagaggg atttaataat
    10081 gtttcaacta taagtaataa tattatattg tacacttgga ttataactgt agtagtgtat
    10141 ttaatttaca ctatatttaa atatataaaa cttaaaaatt taataaatga cttatcttat
    10201 gatgttgatg atagagagat tgtaaatctt tataaaaata tattagaaga atttaatata
    10261 acaaaagata taaaactcaa atactcttat gaagttgaaa caccagcatt ttttaattca
    10321 tgtgtacttt tgccaccaca tgagtataaa ttgaaagagt tagactggat ttttagacat
    10381 gagcttatgc attttaagag tagggacctt tatttaaaat atgtaatcct gtttttaaaa
    10441 acagtatatt ggtttaatcc atttatatat ataatggata aacatataga tttagattgt
    10501 gaactttatt gtgatgaaag agttttaaaa aatcgaaatt cagaggaaaa aaaggattat
    10561 gctttaacaa ttctaaatgc aatgagaaaa ggttcaaata cttcaaataa atttatagct
    10621 ggactccata agcaatctga tattaaaaag agagttatga atatgtttaa tgaaaagtac
    10681 aagaatggta tattaatggc attaacttta tgtttattat cttctattac ttttttgaag
    10741 gttaattcaa cgagtattgt taatcttaat gtattaccta aaggatttga agacttacga
    10801 actgttagca gtatacgttt ttcagttata ggatttacat atgcagatgc tcctaaaaat
    10861 atgagggaag aatataaaat aaattgtgaa tcattaggtt tgatacctaa agactctgat
    10921 aaaatagaag ttggtgtaga atattatgac agtctattga atgattaaat aaatattatt
    10981 ttaacagata ttaattataa agttttgaat agtaatttgt tgagaaataa atatatcctt
    11041 ataaaaatag atacttatat accaagaata aaaaatagtt aagatagtac acaaggatat
    11101 acttataaag aacttaaata agtgaaataa attattaatt ataaggttta taattttaaa
    11161 aaattagata aattcaggtg taaaatacta taaaacacaa ttcggaaaag tgtatgttga
    11221 acttcattta aaatagaatt ttcaaagttg ttatagtaag tagtttaagc attcttctta
    11281 tattaatctt tgagaataat gtacttaaaa aagttagtaa aaaatttaac tattgtatat
    11341 taatgatagt tgctattgat atattatcta aaaaaattaa gtatgaaaag attactgcta
    11401 ggacaatctt ttcatactta attttttaat atttatattc tccaaaattt tcatcttttt
    11461 aacttagaat tattagaatt tgagaatcac tattaggaaa taggttttga tttatgcagg
    11521 gttaaaaatt tcacagaata tctaacttta tactaaatcc atcaaaaaat atacgccata
    11581 taaaagcgca taaaaaaaca cataaaacaa tatttatgcg cactttaacc tcgaatggta
    11641 caaaattctt tacttttaca gacccatatt atataattaa gtaaataatg tatatttagg
    11701 tgacatattg gataagctaa tttatttatt ttattaatat aatgatacta taaaagttat
    11761 ataaagtggt ttacaaaaaa gttacagata gagcttttat taatgtattg tgtattagta
    11821 ttttatcctt aggtacactt cacctacaaa atagaatcat aagataatta tgggaaaatt
    11881 ttaggaggtt tatcgtgaag aaaaagatat taataccagt atttgcatca gttatggcat
    11941 tatcagtaag ttcaattgta aatgcagatg aagtaaatga ttcatctcaa aataaggatg
    12001 ataaaacaaa tactgaatta aatttaggtg aatataaaga agtaaaatat aaagttgcaa
    12061 aaataaaaga tggagtagct ataaagataa gagaagaagg tcaagttcaa aacatagctt
    12121 attctggaga tgaatttacg gttttaggaa ctcaaggaga atgggttaag gttaaagttg
    12181 aagatggcga aggttggtta gctactagat atgtagatat atctgaaggt gtaggatata
    12241 ctaacgctga taaagtaaac ttaagaaaag ataagagtga aagttcagaa gtaattgaag
    12301 agctggaaaa aggaagttct ttattggttt tggaggataa tggagattgg ttaaaagtta
    12361 aagatgggga aacagaagga tatgtaaaat catcttatat atctgataaa gctcctgtaa
    12421 tagaagagcc acaagtaaac ccaagtattg accaaaatgt agattctaca aataataatc
    12481 aacaaaacaa tgcaaatact aatcaacaaa ataataattc aaatgtgcca acagctaact
    12541 ctaatgcagt tcaagctgta cttaacttgg catattctaa gcaaggttgt ccatatgttt
    12601 ggggagcaga aggaccaaac acttttgact gttctggatt tacacaatat gtgtatagaa
    12661 atgcagtagg aaagaatata ccaagaacat ctaaagcaca atctaaatat ggacaaactg
    12721 taagtaaagc aaatttacaa ccaggagact tagtattctt cacaactaat ggttctggtt
    12781 ctgttagtca tgttggaata tatgtaggtg ggggtaatat gatacattca ccatcaacag
    12841 gaaaaacagt tagtgtaact agtataaatt catcatacta tacagcaaga tttgttacag
    12901 caaaaagaat attataatcg gtttgtaata tgacaaattg ataaatacat aaatatatat
    12961 aaacgctcta tattatattt aatttaatca tggaaacaaa aaatgattat attttaaatg
    13021 tactatagag cgtttttgta tcactttaaa tctaatcaag attcaagtta atatgactat
    13081 taaataggag ccttttcata tgatgattat ttgcgcaatc ccttgacagg atagggtaaa
    13141 ggtggtaagc tactaacagt acaaaaatac ttttaatttt agttcagaga ggctaaaaaa
    13201 ggaggacgaa agatgttaaa atcaagaaat ttaatccaac cagaagactt ttcaatagaa
    13261 gaaatagatg aaatattaga attagctcaa aaaataattg acaatccgtc aaaatattct
    13321 cgtatatgtg aaggaaaact attagctaca ttattttatg aaccatcaac aagaacaaga
    13381 ttaagctttg aatctgctat gaacagatta ggaggacgtg tagtaggctt ttcagagcca
    13441 aattcatcct ctgcatcaaa aggtgaaact ctaggagata caatgaggat agtatctggt
    13501 tatgttgata taatagctat gagacatcct caatcaggtg cagcttcaga agcagctaga
    13561 tatacggaag tgccatttat aaatgcagga gacggcaaaa accagcatcc aactcaaaca
    13621 cttacagact tacttactat aaaatcatta aaaggaactt tagaaagcca tacaatagga
    13681 ttatgtggtg atttaaaata tggaagaact gtgcattctt tggttaaggc tatggcaaga
    13741 tataaaaata caaagtttgt atttatagcg cctgaagagt taaaaatgcc agattatata
    13801 aaagaagcta taaagggaca tgcatattat gaaactaata atttggatga tgttatagga
    13861 agcttggatg ttttatatat gactagagtt caacaagaaa ggtttgaaga taaatcagag
    13921 tatgaaagac ttaaaaacta ttatatttta aataaagcta aattagaaaa ggcatctaaa
    13981 gatatgttag taatgcatcc attaccaaga gtaaatgaaa tagacataga tgtagatagt
    14041 gatgatagag ctgtatattt taaacaagct aaatatggaa tgtatgttag aatggcttta
    14101 ataataaaac ttttaggtat aaatgaagac taatagttat catataatag taaaacaaag
    14161 gagattttgg gatgtataaa attctggaaa atatatatat aggcgaagat atgtatagaa
    14221 tgaaagtaaa aggtaatttt gaaggtaaaa tgggacaatt ctatatgtta agagcatggg
    14281 atacttatcc agtactttca agacctataa gtatacatga tatagatgaa gaagggataa
    14341 cttttcttta taaagtagta ggagaaggaa ctcaaatttt gagtaaccta aaggtaaatg
    14401 ataatataaa attagaaggc ccttatggta atggatatgc taaggttgat ggaaaggtag
    14461 ctctagtagg aggaggaatc ggtgtagctc cactatattt ggttgctaaa aatataaaaa
    14521 attgtgatgc atatcttggg tttagagaag atgttatatt agaagatgaa tataagcaag
    14581 tatgtaataa agtctataca acagtaggaa atacttttgt aacagatata attgatgttg
    14641 agaaatatga ttatatatta acatgtggtc caactccaat gatggaaaaa ttagttaaga
    14701 tggtagaagg tactaaaacg agaattatgg tatctcttga aaaccatatg gcttgtggag
    14761 taggtgcctg tttagtatgt acgtgtaaaa ctaatggagg aaacaaaaag acttgtaaag
    14821 atggtccagt gttctgggga gaggacgtga tttttaatgg gtaatttaag tgtgaaattt
    14881 ggaagtgtag aatttaaaaa tccagtaata atggcgtctg gaacttttgg atttggaaaa
    14941 gaatataatg agatttatga tatacaaaaa cttggtggta taagtagcaa gggtcttact
    15001 ttaaataaaa aacctggtaa caatggtatg agagttcatg agacaccatc cggtatgatg
    15061 aatagtgttg ggcttgaaaa ccctggagtt caaggattta tagactatga attacctttt
    15121 tttagtaaac tagacttagt cagaatagct aatgttggag gtgggacatt agaagactac
    15181 ttattagggg ttcaaatgtt aaatgacaag cctatagaca ttattgaatt aaatatatct
    15241 tgcccaaatg tcaaagctgg tggaatggct tttggtataa aaaatgaagt ggcaagagaa
    15301 gttgttcgtg aggtaagaaa cataacgaaa ctgcctttag taataaaatt atcacccaat
    15361 gcagaagata ttgtaggaat ggcaaaagtt tgtgaagaag aaggtgctga tggagtttca
    15421 ttagttaata cttttaaagc aatggcaatt gatataaaaa atagaagacc agtatttgaa
    15481 aatgtatatg ctggattatc tggaccagct ataaaaccta tagctttgag aatggtacat
    15541 gaagtgtgta aaaatgtaaa tatacctgta atgggaatgg gcggaataac taaagccaca
    15601 gatgctatag agtttataat ggcaggagct acttgtatcc aagttggaac tgcaaatttt
    15661 atgaatccta gaataggtat tgaaataata gatgggatta atgaatttat ggatagagaa
    15721 ggaataaaaa gtttagacga aataagagga attatataag gtagaataaa tagtatttta
    15781 aagataaaaa atatagatta aataaacaat aattttataa cattaacatg gaggaaaaat
    15841 aatatgagta aagtagatgt agtggatata ttaaagaaaa gtgatgcatt gttggaggga
    15901 cattttttat tatcttcagg aaaacacagt aatagatacg tacaatgtgc aaaagtatta
    15961 agatttccag aatatgctgc tgaggtatta agtacagttg ttgaacaaat aaaagactta
    16021 gatatagact tagtagtagg accagctatg ggtggagtaa tagtttctta tgagttagga
    16081 agacaattag gaaaagaagc tgtatttact gagagaaaag acaatacaat ggagttaaga
    16141 agaggatttg aagttaaaaa aggagcaaag ataataattg ctgaagacgt tgtaactact
    16201 ggtaaatcaa ctatggagac aaaaagagta ttagaagcct taggtggaga agttgtaggt
    16261 gttgcatgta tagcagatag aactaatcat gatataggta tgcctatata tagtgctata
    16321 aaacttgata ttcaagttta tgaatctgat gagtgtcctt tatgtaagga aggaaaatta
    16381 ccagttgtta agcctggaag tagagagttc aaggaattag ggatgtaata aaaacttaat
    16441 tatttatagt gttacttaaa aaatgtaaat tttttagagt aaaaatttgt aatataacat
    16501 ataacttaaa aataaaaagc agttagcctt ataaatgaag gttagctgtt ttttttgtta
    16561 aaaatatatt tacaaaataa gagatagtag cagtacaata taagtataag gtaaaagtat
    16621 tatatattag ggaggggtaa gtatgaaaaa tcaattaaat gaaaatcata aaaaggttgt
    16681 attttttgga gtaggtgctg taggagctac ttttgcagaa caatttttta actctaaata
    16741 tgattttaaa attctttgtg ataatgaaag aaagaaaaga tatttagaag aaggatttat
    16801 aataaatgga aaaaggtatg attttaatta tgtaactaaa gatgagtata aacaagaggc
    16861 tgattttata attataggtc taaaatataa taatttaaaa gaaaatataa aagaattaga
    16921 tggattagtt ggaaaaaata cagttataat gtctctgcta aatggagttg atagcgaaga
    16981 gataatagga gaaagatttg gaattggaaa aatggtatat tcatatgtta ccaatataga
    17041 tgcaaagaaa atcaataata atattataca cactactaat gggataattg tatttggtaa
    17101 caaagataat agtgaagata gaaaaactaa tataataact gaagtctttg atgatgtaaa
    17161 tatagaatat attttatcaa aagatattca acgagatatg tggtggaagt acatggttaa
    17221 tattggtgta aatcaaactt cagctatact tggtgcacct tatggagttt ttcagagttc
    17281 tgagcattta agagaattag caaaatctgc aatgagggaa gttgttgcta tagcacaagc
    17341 aaaagacata tctcttacag aagatgatgt tgaaaattca ttacatagaa tactagaaca
    17401 ttcaaaagaa ggaagaacat caatgcttca agacgtggaa gctcatagac ctacagaagt
    17461 agatatgttt tctaagaata tctgcaaact tggaaaaaaa tatagcatac ctactcctat
    17521 aaatcagact ttattttata tgataaaagt aattgaaagt agattttaaa gggtatttgt
    17581 aagaaatatt gataattatt gattttaaat tttattaatg ttatatacta tttaagttac
    17641 taaatatttt taactcttca atgaaaatag ttgctgcttt tgacaaagtt atatcttttg
    17701 gaaatatata tgagaaatgt ctgttgtagc tgcttccaag ctctatgaca gacagctcat
    17761 tgtttaaaac aggtaagctt gtcactagat tagaaactat tgtaattcct aaattatttc
    17821 taacagattc tttgacagca taattacttc caaaaaccat catacttttt ggaataattt
    17881 ctttaacact taaaaacata tctaaatagt ctctggttcc agaaccatcc tccctgacta
    17941 tccatttttg gtcttgaagt ttatcaaaac taaaatcttt taataaatga ctattgtatg
    18001 gaagtgctaa aaccatctta tcttcaaaaa aatattcttg aataaatgaa ggggaagagc
    18061 aagtgccttc aataagacca atgtctaaaa tataatcctt aacatgagaa gatacgattg
    18121 atgtattttt tataaaaact tctacatata tgtcaggata cttttgagaa aataatgcta
    18181 aaaaatttgg tagaatatat tctccaatag ttaaacttgc accaatcttt aagtgaccag
    18241 ttatagcatc tgaaacttgg cttacatcat gataagtaat atttaaaaga tttaatattt
    18301 ctttagctct tttgtacaat atttgtccac tttctgttat aaaaatggtt ttttgtttaa
    18361 tagacctgtt tataagcaca actttaaaat aattctctag attttttatg tgtttactta
    18421 cacttggttg tgatagattt aagtattccc cagctttagt aaagtttttg tattcaacaa
    18481 cagctacaaa tgtttttaat tcttcaaaca attttttgtc tccttcacct aatgaaattt
    18541 tacatttttt atatactttc tacagataat tatactatat ataataacca ttaaaaacat
    18601 taatcattac gatattatat atttatttta cttatggtta ttacaattat atactattgc
    18661 tatgaagaaa actatattaa ttataaggta ggtatattat gttaaagaaa aaaagcagtt
    18721 tgacattaaa aactataagg gaaatattac caggactgtt tatatcagtg ttagtaggtt
    18781 atattagtat atttatttct attttaattc ctaaggtagg ggcagcatca atttctatat
    18841 ttttaggtat gtttgtagga aacctatttt taaatcaaaa ggtatttcaa aaaggttata
    18901 aattttcaga gacagatttg ttatcatatt caatagtact attaggggca acactcagtg
    18961 tatctacctt aatagactta aaagtttcag gaattttatt tataatattg caaatgacaa
    19021 taacaataat agctgcactt tatataggta aaaaactagg atttgaagaa aactttagat
    19081 ttctaatggc aagtggaaat gcagtatgtg gttcttctgc cataggagca atagttccag
    19141 tggttaatgc gacagataaa gaaaagggaa ttgcagtaac tattgtaaat gttactggta
    19201 tattccttat gtttttactt ccaataatat ctcaattttt atatagccat gaacttgtaa
    19261 aaacttcagc aatgataggt ggaactttgc aatcaattgg tcaggttgta gcaagtggag
    19321 caattgttgg agaaaatgta aaagatttgg ctactatttt taagatagta agagtaatat
    19381 ttctagttgt tgttgtatta gtatttggac atatgaaaaa tcagtctaat agggaaatcc
    19441 ttgaagaaga aaaggatgag ataaaaaaga aaaaagtaac tataccatgg tatgtaattg
    19501 gattctttat aacttgtgca cttttttcaa tgaacattat accaaaagaa gtatctgtgt
    19561 tgtgcaaaga aataagtaac aaactagaaa taatagcttt agcagcaatt ggattaaaag
    19621 ttaatgtaaa agatttagtg aaacaaggga aagaagtttc tttatatggt ttatttgtag
    19681 gtacagtaca agttgtatca gctgtagttt taataaaaat atttatatag cacaaaaata
    19741 gtatttgtaa tattaatttg attaattaaa ataatatgat actaataatt tataatataa
    19801 ataaaaaatg aatttcgata agtttaagtg ggtataaact tatagaaatt cattttttat
    19861 ttaaggtaaa aatttaaagt aaaatattat tttacataat aataacctta taaattttga
    19921 aattacttat ctttgtgtta aaataagtaa aatacacata ttaaagttaa gtttttaggc
    19981 aaataaacaa aatgaaaata taaatattaa ggagatttgg tatatgaatg tttatgaaaa
    20041 aggtaatgga gttatactag aaggagtaac agattttgac ccagtacata tatttgagtg
    20101 tggtcaatgt tttagatggc acaaacaaga agatggctca tacactggag ttgcaagggg
    20161 gagaatatta aatgtcaaaa aagagaatga taaaatatat ttaaataata cgaatttaaa
    20221 ggagtttaac agcatttggt ataattactt tgatttagga actgattata cagaaataaa
    20281 gaataagcta aaaaatatgg atgaatattt gaataaggct actgaatttg gatggggaat
    20341 aagaatatta agacaagatg gatgggaaat gcttatatca tttataattt cctctaataa
    20401 tagaattcct atgatacaaa gagcgataga aaatttatca agaaagtttg gtaagtatat
    20461 aggtgaatat gaaggtaatg aatactatgc attcccaaca ccagaagaac taaataaggc
    20521 ctcacaagag gaaataagag catgtcaaac aggttttaga gataagtaca taaaaagtac
    20581 tacacaggca gttatagaga ataatgatga ggtatctgaa tatacaaatt taagtacaga
    20641 ggattgtaga aaagagcttt taaaatttaa tggagtagga ccaaaagtat gtgattgtat
    20701 tgcattattt ggtatgcaaa aatatgattc ttttccagtt gatgtttggg ttaagagagt
    20761 tatgcaagaa ttttatatag atgaagacat gagtttacct aaaatgagaa cttatggaat
    20821 cgataaattt aaagaaatgt ctggatttgc tcaacagtac ttattttact atgcaagaga
    20881 actcggtata ggaaaataaa ttaaggggga gaaattatgg gagtaatagg aacaatattt
    20941 ctgttctatt taattatatc atatctagct ggagccataa tttctgtgat tatattgctt
    21001 gaaaatagag accctgcaaa aacaatgtct tggctattga tgtttattat atttccaggt
    21061 gtaggtttaa tgatatatgc tatttcaggt agaaatatta gaaaaagaaa attatttaag
    21121 actcaaaaac tagctaataa tataaaagaa aagaaactat ttgatacatt agaaaagata
    21181 acagaaatag ttgaattaga aaaagaatct ataaaacaaa ataagctttt aagagatgaa
    21241 gaggatggta gttatagaaa aagggtaata aatatgttgt taaagactgg tatgtttccc
    21301 tttactaaaa ataataaagt ggatgtattt gtagatggaa atgaaaagtt taaaagactt
    21361 atagaagata taagagaagc taaagaccat attcatttag agtactttat catcaaagat
    21421 agtgaaatag gtagggtgtt aaaagaagaa cttataaaaa aggctaaaga aggcattaag
    21481 ataagaattt tatatgatga tgtaggatgc tggagatttt ggtttaatag aaaatttttc
    21541 cgagaaatga gggaagtagg tatagaaatt gcagcatttt tacctacaaa atttcctata
    21601 ataggaggga aattaaacta tagaaatcac agaaaaattg ttgttattga tggaataatt
    21661 ggatatacag gtggaataaa tataggagat gaatatttag gcaaaaatga taagtttgga
    21721 tattggagag atactcatat taggattaag ggtatttctg tatacatgct tcaaatgaca
    21781 tttctgattg attggtatta tacgacaaaa gaagtattag tgactaaaaa ttatttccca
    21841 agtgtaggga atgttggtga aagtatgata caagttgttg cgagtggtcc agacagtgac
    21901 tgggaagata ttcattatgc ttatttttct gcaatatgtc aggctagaaa aaatgtttat
    21961 atagaaactc catattttat tccagatgaa agtctactaa aagctataaa gagtgctgct
    22021 cttagtggag ttgatgtgag aataatattt cctaaaattg cagaccacaa gatagtaaat
    22081 atagcatctt attcatattt tgaagaaata ttaagagcag gtggtaaagt ttatttatat
    22141 aataaaggat ttatacattc taaagtcgta ataatagatg ataaaattgc ttctgcaggt
    22201 acagctaata tggatttaag aagttttatg cttaactttg aggtaaatgc atttatctat
    22261 gatgaagaag ttattagggt aatgacagat gatttctttg aggatttaag tcactgtgaa
    22321 gaacttaatt tggaggtatt taagaataga aatatcatac aaaaaatcaa agaatctgta
    22381 gcaagattat tttcaccaat attataaaaa attattaaaa aaggtattga aaattatgtg
    22441 tgattggtat attataataa ttgttagcac tctattagat agagtgataa taaataaaaa
    22501 gtatatatat aaatctacat atataagtag tattaattta aaaataacta aaaacatagg
    22561 cgaactagtc tatacttaat attttaggag gcgtgaataa tgaaaataag accattagct
    22621 gacagagtag taattaaaaa agtagaagca gaagaaaaaa ctgcaagtgg aatagtttta
    22681 ccaggagcag ctaaagagca acctcaaata gctgaagttg tagaagttgg accaggtgga
    22741 atagttgaag ggaaagaaat aaaaatggaa ttgacagtgg gagataaggt tatattccaa
    22801 aaatattctg gaacagaagt taagatagaa ggacaagaat acacaatact aagacagagt
    22861 gatgtattag ctgttattga ataaatatag aataaattta ttaggaggga tttaaaatgg
    22921 ctaaagaaat taaattttca gaggaaacaa gaagagcttt agaagctggt gtaaataaat
    22981 tagcagatac agtaaaagta acattaggac ctaaaggaag aaatgttata ttagataaaa
    23041 aatttggttc tccacttata actaatgatg gagtaactat agcaaaagag atagaactag
    23101 aagatagatt tgaaaatatg ggtgctcaat tagttaaaga agtagcaact aaaactaatg
    23161 atgtagctgg agatggtact acaactgcta cagttttagc acaagctata ataagagaag
    23221 gtttaaagaa tgtaacagca ggggctaacc caatactttt aagaaaagga atacaaaaag
    23281 cagtaatagt agcggtagaa gaattaaaaa atcaatcaag aatagtagaa acacaagagg
    23341 ctatatctca agttgcttct atatctgctg gtgatgaaga agttggaaaa ttaatagcag
    23401 aagctatgga aatagtaggt aaagatggag ttataactgt tgaagagtct caaactatga
    23461 atactgaatt agatgctgtt gaaggtatgc agtttgatag aggatttgtt tctgcatata
    23521 tggttacaga tgtagataaa atggaagcag ttttaaatga cccatatata ttaattactg
    23581 ataagaaaat atctaacata caagaattat taccagttct tgaacaaata gttcaacaag
    23641 gtaaaaagtt attgataata gctgaagatg tagaaggtga agcattatct acattagtag
    23701 taaataaatt aagaggaaca tttgatgtag tagcagttaa agctccaggc tttggagata
    23761 gaagaaaaga aatgcttcaa gatatagcaa tacttacagg tgctcaagta atatcagaag
    23821 aattaggtta tgatttaaaa gaagctgatt tatctatgct aggtagagct tcatctgtta
    23881 aggtaactaa agaaagtact acaatagtag atggttctgg agataaaaaa gctatagaag
    23941 atagagtaac tcaaattaaa catcaagtag agcaaactac ttcagatttt gatagagaga
    24001 aattaatgga aagattagct aaacttgctg gaggagtagc tgttgtaaaa gttggagctg
    24061 ctacagaagt agagttaaaa gaaagaaaat taagaataga agatgctctt aatgcaacta
    24121 gagcagctgt agaagaagga atagtagctg gtggaggaac tgcttttgtt agtgttatac
    24181 cagcaatagg aacactaatt gagagtttag aaggagaagt taaactaggt gctcaaatag
    24241 ttaaaaaagc gttagaagag ccattaagac aaatagctat aaatgctggt cttgaaggtg
    24301 ctgtaattgt gcaaaatgtt gttaattctg aagcagaaac tggatttgac gcattgaatg
    24361 aaaaatatgt aaatatgata gaagctggta tagttgaccc aactaaagtt agtagaagtg
    24421 ctttacaaaa tgctgcatca atagcaagta ctttcttaac tactgaagct gctgttgctg
    24481 accttcctga aaaagaagat gcaggaatgc caggaatggg tggcggaatg cctggtatga
    24541 tgtaatttaa aaaatttaaa atataaattt taatataaag aactatttcg acttagattt
    24601 taaactctaa gttgaaatag ttcttttgta tatatctaat ttttgtatat ctataaattc
    24661 aatacacata tttagataat cagtatctag tttgtaataa tattttagag tagtattgtt
    24721 attttttagg ttatgatttt agatatatat taattaaggt ataattgttg atagagaata
    24781 aatcaaattt aaaaggatgc gataatatgg acttaagaaa attacttgaa gaagttaaaa
    24841 atgaaaatat tgatatagat tttgcgttag aaaagttaaa agatttacct tatgaagacc
    24901 ttggatatgc aaatatagac catcatagag aattaagaaa tgggtatccg gaagttattt
    24961 attgtgaagg taagagtgat gagcatattt tagggattat tgataaaatg aatcaaaaaa
    25021 gttctaacat attggggaca aggtgcagaa aagaaacttt tctgaaaatt aaagaaatct
    25081 ataatcatgc tgagtacgaa gaggcgtcta aaattcttaa gattcaaaat catgatatag
    25141 agaatatagg aaaaggtaaa attgtgattg ctacaggggg aacttctgat ataccagtag
    25201 cagacgaagc ttatcataca gctaagtttt taggtaatga tgttgataga atatatgatg
    25261 ttggagtggc aggtatacat aggcttttaa ataaaagaca taaaatagat agtgctagag
    25321 taatagtagc cgttgcaggt atggaaggtg ctcttgcaag tgtaattggt ggactggttg
    25381 atgttccagt aatagctgtt ccaacttctg ttggatatgg agcaaatttt ggaggattag
    25441 cagcattact agctatgtta aatagctgtg catctggaat atctgttgta aatattgata
    25501 atggatttgg agcaggatat ttagcatcaa tgataaataa actgtaaata cattgatata
    25561 aattaaacaa aactatataa taaaatttaa tttctattat atttttacta ttaaaattga
    25621 ggagacatat atgagtgaaa aaatacttta ttttgatata ataagcggta tatcagggga
    25681 tatgaccctt gcaagtttat taaatttagg tgtgcctaag gaaatttttt tggaggaaat
    25741 tcataaacta agaatgagcg atgaatttaa tataaacatc agctcgaaaa ctgaaaatgg
    25801 aatagtaggt acaaaagtag aagtaattac aaaagaaaaa catacacata gaaatttagt
    25861 ggatatattt gaggttatag ataaaagcac tctaaacgaa aatgtaaaat ctaaagcaaa
    25921 aaaaatattt atggtaattg gagaagcaga agcaaaagtt catggaacta caatagacaa
    25981 gatacatttt catgaggtag gggctataga ttcaattgta gatatagtag gagcttctat
    26041 attagttgac ttactttgtg tagataaagt ttgtgctagt acagtgtctg ttggttcagg
    26101 atttgtaaaa tgtgaacatg gaattatacc tatacctgca cctgcaacta tagaaatttt
    26161 aaaaggtgtg ccaattaaat taaataatgt aaatggagaa tgtacaactc caacaggagc
    26221 tgcaatcata aaagtcttgt gtgatgagtt tgtagatgag cttgaatttt ctccaaagca
    26281 aattggatat ggtataggtc ataagaagtt tgaagtacca aatatactca gaactgtatt
    26341 aggtgaaaaa aaaaagaaga attaatttat gaaattagtg caaacattga tgatatgtct
    26401 tcagaaatct attcttattt gtatgaaaaa atattagatg agaaagcatt ggatatttat
    26461 acagagagta tttatatgaa gaagaataga ccagcaacaa aagtatcaat tttatgtaat
    26521 gaatcagatt tagaaaagtt tattaaaata ttacttttag aaactagtac ttttggagtg
    26581 agatataatg catataatag agaaatttta gagagaaaat ttgtaaaatt agatacaaaa
    26641 tatggagtgg ttacagtaaa actagggtat tataaaggaa aactagtcaa ggttactcca
    26701 gaatataatg aatgtaaaac tatagcaaaa aatgaagata ttcctataaa aaaagtgttt
    26761 gctgatataa attttagaat aaaagaagaa tttagtgaaa acttattgac ataagtaata
    26821 aattttgata aattaatatt cagccttatt aaataaaagt aaacagaaaa aaatatataa
    26881 aatataaact cactcgtata tgctcagaaa tatggtctga gagtctctac caagataccg
    26941 taaatatctt gactatgagt gaaattatta taccaaaaga cctagttaag taaatgtgta
    27001 ggctgagtta tactccctat gtgttggcta taagataaag gtattttgga agcctgtata
    27061 gtaatttcac ttttgctaga aattatggaa gttactatac aggttttttg tgtaaaaaga
    27121 tttaatataa tttagactgt attgtgttaa aataaatata taaaaactat attatacaag
    27181 gagagatgtg aatttatgaa acatgaatta gtacttgtaa tagattttgg aggacaatat
    27241 aatcagttga tagctagaag agttagagaa aacaacgtat attgtgaaat acttccatgt
    27301 actgcaagca tagaaagaat aaaagaaaaa aatcctaaag ggataatatt tacaggcggt
    27361 ccaaatagtg cgtatttaga agattctcca acaatatcaa aagaaatatt tgaactagga
    27421 gtaccaatac ttggaatatg ctatggtatt caaataatgt ctcatgtatt gggtggagtt
    27481 gtaagaaaag gtaataaaca agaaaaggaa tatggaaaaa catctataac ttatggaaaa
    27541 tcatcattat ttgaaggaat aactacaaat agtgtatgga tgagtcatac agatttaata
    27601 gaaaaagttc cagaaggatt tactatagtt gcaaatacta atgattgtcc agtagcagct
    27661 atggaaaatg tagaacgaaa cctatatgga gttcaattcc atccagaggt agaacactgt
    27721 ttggaagggg acaagatact tacaaacttc ttatataata tttgtaaagt aaaaggtgac
    27781 tggactacag attcattcat agaagataaa ataaaagaat taaaagaaaa aataggcgat
    27841 aaaaaagctt tatgtgcttt aagtggagga gttgattctt ctgttgctgc agttcttatt
    27901 cataaagcaa ttggggacaa cttaacttgt atatttgttg accatggttt acttagaaaa
    27961 aatgaaggaa atgatgtaga aagaatattt agagagaaat ttgatataaa tttaataaga
    28021 gttaatgcag aagatagatt cttatctaaa ttaaaaggag tttccgaacc agaagctaag
    28081 agaaaaataa taggtgaaga atttataaga gtatttgaag aagaatcaaa taaattagga
    28141 aaaatggatt tcttagtaca aggaacaatc tatccagatg ttatagaaag tggacatgga
    28201 aatgctgcta ctataaagtc tcatcataat gttggtggaa tcccagaaga tgtagacttc
    28261 caagaaattg tagaaccatt aagagagtta tttaaggatg aagttagaaa aataggatta
    28321 gagcttggta tagaagaagg attaatattt agacatcctt tcccaggacc aggtctagga
    28381 ataagagtta taggtgatgt aactaaagaa aaatgtgata tattaagaga agctgatgct
    28441 gtttatatgg acgagcttag aaaagcaggt ttatacagag aaatatggca agcttttgca
    28501 acattaccag atgttaagac tgttggagtc atgggtgatg aaagaacata tgcttattta
    28561 gttggtttaa gagcagttac ttcttctgat ggaatgactt ctgactggta taagatgcct
    28621 tatgatgtat tagaaagaat ttctaataga ataataaatg aagttgatgg agtaaataga
    28681 gtagtttatg atataacttc taaacctcct ggaactattg aatgggaata aaatatagaa
    28741 tgccagaaat ccacatgaat acaggatttg aggtctctct tacttctata ttatactggt
    28801 ttgatactat caatatttgc cagtatctta tggattaata agcaaaaaac atcctgctga
    28861 atggtaaatt cggcggggtg tttttatttt gttttgaaac tcctttgctc tgaaactcga
    28921 agtatttaac actgatgatg ttgttatctt ttagttcctt ttgtgtcatg ctctgaaaga
    28981 caaattgata caataaaaaa gtaatttctt aatcattgat ggagatatgt atttatgtta
    29041 ctcttgatgg taatttgagt aatattgttc atagcttttt atattcactt aattagaaaa
    29101 ccatttggta taatcataaa taaacttgat gatataatag aaaagattta taattattag
    29161 aagtatataa aagtatttta tgtctatgaa atgtatgtct ataaaacata tagatgactt
    29221 ttgtatatta atgtatgaat attgatatta tagaagaatt gagttgtcaa tgatactaaa
    29281 tcaaatattt atagaagatg gagggattat gagaaataaa ataattatag gaattacttt
    29341 ttcacttgta ttactaatgt ctggatgtgc ttcaaaacaa acagcagaaa taaacgtatt
    29401 agcaactact gatttacatg gagttatacc ttatgagtta acttcttatg taaaagaaga
    29461 aagaaaaaaa gataaaaatc taactttagt agatgcagga gatttttttg atagtggaga
    29521 agtgtttggt tctagcatgg atagttattt tagtgctaga tataaagatg gtgataataa
    29581 cactgaaagt tatattgaga cgcctatttc aaaagatatg aaagaggtag gttatgatgt
    29641 tgcagttctt gggaatcatg aatttatatc caacaataaa ttttatctag ataatatgat
    29701 atctgatttt gagaaacaaa aaataaatat tttatcggca aatacgtata aaaaaaatgg
    29761 agatagttat acaaaaccat acataataaa aaatattaat acacctgaag gaaacgtaaa
    29821 actaggaatt ttagggctta ctataaaaga agttggtgag aggaaacagt ggaaagatgg
    29881 aaaacttgtt gactctaagt ctttagaact aaaagaccaa gagggctaca atggtgaatt
    29941 gtatatgaat gatttagtag aagatgctaa aaagtgggtt ggtataatgg aaaaagagaa
    30001 gcctgacatt atagttgctg tggcacatac aggtgagaaa cctaagaaac ctagaaaccc
    30061 aggaaataga atacaagact tagctcaaca agttgatgga attgatgcta tagttgcagg
    30121 tcataatcat gttcaaattg agcaacatga ttataaaaat aaatcaggag aaaatgttat
    30181 agttactgag ccaggaaagc atggggaata tatttctaag attaatttta aattagaaaa
    30241 aaacaatgat aaatggaata tagttgacaa atctagtaaa ttaattaaat ttgaaaaaaa
    30301 tcaagaaagt gataaatttg gagaatttat ttcttcaatt aataaagtaa gtaaagataa
    30361 gaaagaaata agattatcag aagtaaagac tcctgaatgg gataaaatat atgtttttaa
    30421 atctggaaca tctgcagatg atatatataa aacaatagga tataaatgga gaaatatatc
    30481 atctccaaag ggtgatgaca tggtgcagat tgttgtaatg aataataata agcctgttag
    30541 ctatatatat ggtgaagctg aagttatgcc aattaatata aaatgtgatg aatcagagta
    30601 taaaaataat gttgtaacta tttatcctaa taaaaatgat aaatttaaaa ttgaaaaagg
    30661 taagcaagaa tatagtatta atttaaaaca tatacaatag aaacttttat cttatttaag
    30721 tttagattgt acttaaaata aagtgtgtat tattttaaaa agtgatgata attaaaatca
    30781 caacattaag gaatagtaca cactttttat gctaaaaaat agaatgttaa aaaattaaac
    30841 ctttaaatta aagtacattg cttatattat ccaatattat ttccaaaggt tgtgtaatat
    30901 ttttatcctt atgtaatatt gctaacattt tattttccat atctatctca tctaattcaa
    30961 tcaatcgcaa ttttttatgt aagagtgaat ctaataataa attttctggc aaaatggtta
    31021 tacctaaccc tgcttctgca gctttaataa gtgcaaaaga attaacactt tcccaaacag
    31081 gataagcttt ttggtttgaa agagataata cactgtccaa agtatcgcga atagcacttc
    31141 cttgttcacg taacaataac ggaaacgtgc ataactgtcg tagagataag gtttgttcag
    31201 ataaaggaaa agttggagca catgcaacac ataacttata ggaacctaat aaaattgtac
    31261 ggaatgcccc ctgtggttct ataccttccc aaaaacatat atcagcttct ccacttttta
    31321 aaatatccat agctgaatta gcactttcga cacgtacatt cactttaaga gatggaaatg
    31381 aattttttag tcgacttaaa attggtggaa gtaaaaaaga ggcaattgta atgctagaaa
    31441 caatgtttat aggtgtacat tcttctaaac gactgatttt tttgtctaaa ttgcgacaag
    31501 cagttaagat actacgtgct tcatctaaaa gagaaatgcc acagcgagta agacataccc
    31561 ctcgtggtaa tctgtcaaat agtgatgttt cagcttgttg ttcaagttca gctatagcat
    31621 gagatacagc agattgtgtg atatgtaatt tttttgcagc acctgtaaaa gtgcctgttt
    31681 gttcaattgc ttctaaaatc tctaaatatc gtatgtttat cataataaat atcctcacta
    31741 tacatgaata aaattaatta attccattaa attctatcat tttacagatt gcaaagcaac
    31801 aggtatactg aataatatga agtgaaaaga aggaggaata ttttgaatca aagcagaaaa
    31861 atatattttt ggcttttaat tattaacttg tttattagca cattcacttt tggcggaggg
    31921 tatgtagttg tacctatgat tagacgttat tttgtaaatc aaaagcaatt ctttacagaa
    31981 gatgatttga tgagtatggc agcagttgca caatcaactc ctggagctat tgcaattaat
    32041 ttatctgctt tggcaggtta tcgagtagct ggtactttag gggttgtcat tagttgtaca
    32101 gcagctataa tacctccact cgttattctt ggattagtat cagcatttta caccactttt
    32161 atatctaatg ccattgtaac tgcagtatta aaaggaatgc aagctggtgt tgctgcacta
    32221 attgtggatt tgattattga catgtgctct atgatattaa aacaacgttc tttgttttta
    32281 tcaactatga tacctattgc tttctttgca aactttgtga tgagtataaa tgtaattttt
    32341 atattatctg tttgctgttt tgtatgtgta atgcaagttt tctataagaa gagggaaaat
    32401 aaatgaatac aatttggaac ttatttttta tatttttaaa aataggcttg tttagtattg
    32461 gtggtggtta tgcaatcata cctttaatcc aagagaaagt agtaaatcaa actggatgga
    32521 taagtgagaa aatgtttaca gatattataa ctatttcaca aatgacacca gggccactgg
    32581 ctgttaatac ttctactttt gttggacttc aaattggagg gttttgggga gcagttgcag
    32641 ccactttagg ttgtgtatta tgtggtgtag taatatctct atttttatat aatttttttc
    32701 aaaagcatca gaactctact tatatatttg aaatattaaa tggtctaaaa tcagcttcat
    32761 taggtttaat tatctcagct gcggctacaa ttataatcct tgcattattt gggacgaata
    32821 aagtgtactt caattctata tttgagatgc taaactggac tgcactaata atcttttttg
    32881 ttatactact gatagtacgt aagtggaaaa ttaatcctat tgtgattata ataataagtg
    32941 gtatactagg aatatttttt tatttataag tttttggtaa gtaaaggata tctatttttt
    33001 tatatcttaa atccaaatag ccaataaagt aaggctattt ggatttttaa taaattctga
    33061 aaagataatc agaaacccat ataacattta aattatatca ctggttttct acaaaccaat
    33121 cataagcttc ttggcaaaat ttctcactat ctatatagta actattatga acattattaa
    33181 tttcaacaag tttcttttta cattttagtg aattataata gctatcaaaa tattgggttt
    33241 taaaatatct atcatgaagt gttttaaaga caagagtagg tatgttgact ccatctttta
    33301 tcttactatc ttctgcaata aagcattcta acattgtatt aattatcttt gtactatttt
    33361 tccaaatatg aggatttttc ttttcaaata ttttataaaa ttcattatca tagacttcat
    33421 tataaccttt atatttaggt ggttttactg ttatgtgaaa attaggaaga aacttaaaga
    33481 aaaaatataa taatttttat gttctgtttt atatattatt catattttat cacagataat
    33541 atactaaagt taattttttt attgtacaac tattataata attcttctat aatataagac
    33601 attctataat tttatattta agttcatgtg tatttaattg tttttatata aaatgtccag
    33661 taatactatt tggatttatt tttatttcat caggagttcc agaagcaaca atttcccctc
    33721 catttattcc actgtctgga cctatatcga taatccaatc agaagcacgt ataacctctg
    33781 tattgtgttc aataattaaa agtgaatgct gttcatttac aagttttcta aaagtaatca
    33841 acaaattttc tatatcttta aaatgaagtc cagtagtagg ctcatccaat atgtacagca
    33901 ttcgtttttt agtttttgtc cctaattctt ttgctaattt tatcctttga gactctcctc
    33961 cgcttaatcc taatgtattt tgtccaagct ttagatatcc aagtcccact tcatctaata
    34021 aggagagttt tctatatata tcagagcaat cactaaaaaa tgtttttgct tctgctactt
    34081 ccatgtcaag tatatctgca attgtttttc ccttgtattg tatagacagt atttcttttt
    34141 gatatctctt accttgacat tcattacaag ttacccattg gtctgccata tattgtagaa
    34201 taatttttgt tttacctaat ccttgacagt ttgggcattg cccctttttg ctattaaagc
    34261 taaaatagct ttcatccaat aatttttgtt ttgcttgtgg agtttcagta aatagagaac
    34321 gaatttcatc aaaaatattg atatatgtag caggtgtaga acggctagat tttccaatag
    34381 gtgtttggtc cattaagata aaatcgtcaa tagcatcaaa tccagtaagt gcagtataat
    34441 ttttatttgg gatagatttt tgtttcaatt tttcttctag agcaggaagt aaactgtgaa
    34501 atacaagaga actcttacca gaaccactaa cacctgtaat agagcacata cagtttaagg
    34561 gaatttctac atcaatattt tttaagttat ttgcatggca tccctttaag cttaaccatt
    34621 tacttggtat aattttattt tgtgaactta tcttattgta tgtactcaaa tatttgcctg
    34681 ttatagaatt tggatttttt ataatttctt gggttgtacc ttctgcaatg atgaagcccc
    34741 cttttgtacc agcatttggt cctacatcaa taatataatc agcagacagt attgtatcct
    34801 tatcatgttc cactactata acagtattac ccttgtctct taattcttct atcattttga
    34861 tgattagatt atggtctctt ggatgtaaac caatagatgg ttcgtctaaa atataggtta
    34921 atcctactaa ttcacttcct agctgacttg ataatcgtac tctctgcagt tctcctccag
    34981 ataaagttgg agctgttcta tctgttgata tatagctaag acctaccttt aatagataac
    35041 taactcgaat ttttaattct gttaagatat catttaccaa agatagctca tttttttgaa
    35101 gttgatttgg taattcacac aaccaattcc ataactgttc tatcgtcatt tttgttagtt
    35161 cagggaagcg ataacctttg attgtagtga atctagcctc aatgcacagc aattcgccac
    35221 cacatgtatt gcaaggaatt ttgttcatat attgatgcaa ggtattattt tcagaactag
    35281 attctctaaa aagtctttga atgtggttga ttgctcctga agcaggcctt ctaatctttg
    35341 attctcgtcc ctttgattca aagctaaatt catatatctt atcgtctgta ccatataaga
    35401 ttgcatctat aaaatctctt ggcaattcat tccaaggtat atctaggtct atatttttat
    35461 cttttgctat tgcatataat tcacctacca tccaattacc atttggcttt ttccccctca
    35521 attttccaaa atagtaagta gctccatcta atattgaaat agttgggtct tttacaatta
    35581 attgaggatt aacttgatag gtaaaaccca atccattaca atcgtgacaa gctccagtat
    35641 gggagttagc attgaaggtt gaagtattta tcttagaaaa aatagtttgg caatgtggac
    35701 aataattttt tgtattattc tttttttcta atggagtttt gcaatatgga cagtaacttt
    35761 ttccgattga tacaaataac attcttaagt aatcccaaat gcttgtaaga gtaccaacag
    35821 tagaacgagg attttggctt ccttttttct gttcgatagc tatagtaggg gttaatccct
    35881 gaagtaaatc aaattccggt ttttgaagct gttctgaaag ttttgctcca ttacctagat
    35941 tttcaaaata acgtcttttt ccttcttcat aaatcgtatc gaaagctaaa gaagattttc
    36001 cactaccact tacacctgta ataacagtta tttgattttt tggaattgta acctcaacat
    36061 cctttaaatt gttttgcctt cctcctttta gatagataga gcttttatta tgacaaagtg
    36121 aatttttaat tgatttattt attgcttttg caaatttgaa tttcttttct agttcaagag
    36181 gattgcctaa ttcttttatt tccgaaatac ctaatatttt taactgagaa aagattgatt
    36241 tcaaataatc ttcaggcaat ttttctatta acaaattaag gtcttctatt tttatatttc
    36301 cgcttgataa tatctctgcc atttcatcta ataaattttt ttctgctctt gttaattgat
    36361 tgtatttttt ggaacttaat tctgtccata aatcccatgg aagcaattct aaaccaatca
    36421 tattattaaa gtcatgtaga agattaccct ttacagaatg ccaacctttc caatttttat
    36481 tatatccaaa gtcatctgga tgagcatgtc cttgtcgaca tcttatccag gcttctgaac
    36541 cagttaaaaa accatctgtt tttccgacat catgagtgtc aaaattaatt tgaaaataat
    36601 ttttttgtat gtcatctatt tgtgcatcta ttcttatcca tctctttgtt agagtatcat
    36661 agtattctac aagccagtgg tcttcataag taagctcatt ttcaaaataa ttagcaaaac
    36721 ctgctctcat tctagcctca aaacctcgat atcttaggag cgatacaaga agtaaagaat
    36781 aatctctgca cattccaaca agcctttgtt ttgggtttct tggaatagtt agtgggttag
    36841 aatttatttc aaatattctt tttaacattt gaggaattgt acgaagcaat tcctcatcag
    36901 attgttgctt attaaaggat ataccatata attttccttg gtctccatga attactaggc
    36961 tttgaaccac ttttaataac tcaattggag tctttggtaa atcttgatat ttattttcaa
    37021 attttttagg atttgtgatg atgctttgat ttttatagtt atttattttc atttatattt
    37081 cacctctcta aatggatgat ataccctgac atggtgtcat atgcaaggac aatttagatt
    37141 ttttggccaa aatttaaaaa taaataaaaa atgatacagt gataaattgt aatacaaata
    37201 tagtttcaaa ttgcatataa tataattatt taataaaacc attatctatg gaatgttaat
    37261 gaaatgttaa gatggcattc ttgaatatta agaaatagaa tactataata tagtgtgaag
    37321 gattcttagt aaaaaaattt gtaagtgtta atatacatta aaaaataggt ttataatttg
    37381 gaggaaaaaa atgaaattat caaggtataa gaaaaatagt ttagttctgt ctataatttt
    37441 atgttcactt attatttcaa tccttgtaat tactgttttt aataccgtaa aactacacaa
    37501 tatatctaat aaacaatcta aaaactattt aaacgatgta tctacacaga ttgtaatgaa
    37561 tgttgattct aaaatcaaat ttattttatc cgacttaaga ataatggcag attttattaa
    37621 gcaatatgaa ggtgatagtc gatatgagta tttatctaaa agaaagacta gttataagta
    37681 ttatgatgtt ggaataatag atttagaagg taaagctgag tttttaagtg gaaaaaagtt
    37741 tgacttaaag aatactgctt cctataaaaa aggaattgaa ggtaaagaat actcagaagt
    37801 aatagagtca atgggttttg ttctttatag tgtacctata tttaacgata atcatgaagt
    37861 gtctagtatt ttaataggtg tatccaataa agaaagcatg gatgagattc ttaatataga
    37921 tagctttaat ggagaaggaa ctattgaaat tattaatagt cagggaaaaa ctcttttcat
    37981 aggaaaaaat aatgagttaa taaaagacct tagctataag tataaggatt ctgcagatga
    38041 accttgggca caaaagatgc ttaatgattt taaaaatgat aaatcaggaa atattacaat
    38101 aacctcatca aaaggtgtac aatgcttatt aacttatcat ccaattacaa gaggtttaaa
    38161 tgattggcat tttcttctca tagtacctga agatgttgta cttggtgaac ttaataagtt
    38221 aaattctttt acaataataa tgacatttat aactacatgt attattggaa ttataacatt
    38281 aattttatat attataagaa gaaaatatgt aaatcaaata gaaaatattg catatttaga
    38341 tagtataaca aatggtataa attcaactaa gtttagtatg ctagtcaaac ctttaatttc
    38401 agaatcacct gatagtactt atatgatgat agcaatgaat ataaaagatt ttaagctaat
    38461 aaatgattgt ttcggtagtg aaaaaggaaa tatgacacta aaacatcttt acaatatttt
    38521 gtataaaaac attgatgaaa atgaagaatt tgtgtgccgt catgatgcag accttttcta
    38581 tttgcttatt aagaatagac cagtgttaga agccctggag ttattatata aaatagaagc
    38641 tgacacaaat tattttaata aaaataggga aaatccatac tttttaagac tatcagtagg
    38701 tatttacaca attgaaaatc ataatgaaga tttaattaca atacaagacc atgcaaatac
    38761 agcaagaaaa agctttaata aaactcatca aagtgatttt tctttttata gtgatataga
    38821 aagaaaacga ttaatatctg aaaaagaaat ttcaaattta atggaaaaag cccttgaaaa
    38881 taaagaattt tttatgtgct tacaacctaa aattgatata aaaagtggaa aaattagtgg
    38941 agcagagtct cttgtgcgtt ggaaaaattc agaaaagggc ataatttatc caagtaattt
    39001 tattcctctt ttcgaaaaaa gtggatttat atgtaagtta gatttatata tactagaaga
    39061 aacatgcaaa ttgatttcta agtggataaa agaaggtaaa gaagtgttga gaatttctgt
    39121 taatgtatca cgtcaacatt tgaatgataa attgttttta gaaaaatata agcatatatg
    39181 caataaatac aatgtaccaa catgtctgat agatttagaa cttaccgaat ctattttctt
    39241 agaaaatcca gaagctatag atataataga ggatattcat tctaatggat ttaaatgttc
    39301 tatagacgat tttggatttg ggtactcatc attagggata ttaaaggatt ttaaagttga
    39361 tataattaag ttagaccgtt cattctttgt tagcaaaaat aatatagacc gtggtaaggt
    39421 agttattaag tctattattg aacttagcaa aaggctaggt atgaaagtaa ctgctgaagg
    39481 aattgaagaa ttagaacaag ttgagttcct aaaaaatgta ggatgtgatt atattcaagg
    39541 ttatgttttt tctaaaccat tattaatttc tgattttgaa aattttgcat atgaaaataa
    39601 taaaattaaa aatatccatt attaaatgta caagataaaa aatttaggca agttatataa
    39661 ttaaaaatat aacttgtctt ttttatttat atatttaata aaaatatatt tgcagatttt
    39721 gtaaaaataa ttttcaaatg ttgtaagttg tggctaaagc tttattgtat tattctgaat
    39781 atagaaagat agttactgtc ttagataaaa actgaaacat acataataat ataaaatttg
    39841 ctatattaat aactctaatt attttggaag gagggaaaat atgataagtg ggagaatgtt
    39901 actcatcatg aattttctaa aaggaaaaaa taagacaagt tacaaggata ttagcaaaca
    39961 acttagtata gaagaaagaa aagttagata tgatataaat aatttaaata ttgttttgcg
    40021 ttcattaaac aagtcagtaa tacaaaagat gaacaaaggt gtgttaatta ttcctgatga
    40081 cttccaagta gtatgtataa ataataacga atatgtattt tccttaactg agcgagtatc
    40141 tattatgaaa attatatcaa tgtttaaaat agataatcta aacttagaaa aattgagtaa
    40201 agaatttcag gtttctagaa gttcaattaa aaatgacctt aatgttttgt caagtgaatt
    40261 agaaagaaac caaataacta ttacttatga taaatctttt aaaataagtg gagaagaaga
    40321 ccttatatat aagcaaagat tgaacatatt aaaatcgtat tcttatcttt ttggtaagga
    40381 aaaatctgat ttaagtgtat ttgagaagta tttagtgaat gtaattgaaa aaatattcaa
    40441 agggatttat ttgattgata tatataattg gtctatgaat ttattattgc aaatgggatg
    40501 gactttaaat gatgaatctt ttacatggta tgtgtcaaac attttccttt ttacatggta
    40561 tatacattca gatgaaaaac atcctttaga atctgcttca ttaagtgagc catgttttga
    40621 aaataagttt gaccaagaat ttgaaacaat tattcaaaaa aagttgacta aggaacaaat
    40681 ttttctgtta aagagttttg tattttttac aaataaatat gcaagtttaa acgaggatat
    40741 ggatttgatt agtacagaaa ttatagttca agacctaatt tacaatatgt cagaaatatt
    40801 agagattagc tttaaagaag atatgattct ttataaagga ttactcaatc atattgcccc
    40861 attgatagag agaatcaagc agaatataca gatttatgag gaattacttc atgtaatacc
    40921 agataaatat caatatgtac ttgaagctac taaggtttct attcaaaaaa acagagtttt
    40981 aagtcaaata aagaatgaaa atgaaataat acttttggca atacatttct taggaagtat
    41041 acatagaaat gagaggaata actacaaaaa tatattgtta gtttgtggat ttggatatgg
    41101 tgtaattgct atgttaaagg atagtttagt aaatgattac cagataaata tagtagagag
    41161 tattccatca tataagttac aagattataa agaatgggaa agtattgact tcattataac
    41221 aacatcaaag atagtaatag acttgcctaa aacaattatt gagataaatc catttttaca
    41281 agaagaggat tatttgaagt ttgaagaaaa agggataaaa agaaagaacg tcttaactaa
    41341 ttatatgtca ataaaaaaaa ggttggactt tttagaagaa gatgctcaaa aaagagtatt
    41401 aagtattata tgtcaagaac ttggatacag tgatgagaga atattattta gacaacttaa
    41461 tttaagtgat ttaataggga ttgataccat aaggataatt gatgaagata tgaaatggga
    41521 agatgctgta aaaattagtt caaacatact tgctaattgt gattttgtat cagcacaata
    41581 tgctgagaat attatagata ttttaaaaaa tgtgggtttt tatgctgtaa aagataacga
    41641 atttgcttta ctacatggca atgatagttc attagtcaaa gtaagttcga taagtttatt
    41701 aatatgtaaa gaagcagtta gttttggaga taaaaaggtt aaaatcatat tttgtctagc
    41761 aagcagagat aaaaaagagc aaattcctgc aataattaat ctaactaaaa tggtttataa
    41821 gactaatttt attgctatgt tagaaagtgc aaagacaaaa gaagaagcag aagttttaat
    41881 tcataaatat gagaaggagg tcactgtatg aataaattag ttaatgtaaa atgttcattt
    41941 aaaatacatg ctaattcaaa agaagaagct attgtatcgt tagttgatgt agtatcaaaa
    42001 gaaggatatc taatagataa aaatcaattt ttggaagatg ttttaaagag agaagaaaca
    42061 ttgtcaactt atattggaca tggtataggt cttcctcata gtcaaagtgt aggtgttaaa
    42121 aactcttgta ttacaatagg aaagcttgat attcctattg aatggacaga agaaggagaa
    42181 aaagtggatt taatattttt aatatcagta acaaaagaca atgaaaataa tcttcattta
    42241 aagattttat caaaattagc acgtttattg atgcatgaaa gttttagaaa tcaaattaga
    42301 tgttcagatg aacaaactgt ttataatctt ataaaagaaa aaatagagga ggaagattaa
    42361 aatgagaatt ttaaagtttt tgcaaaaaca tcttatgaca gcaacatctt atatgattcc
    42421 atttgtggtt gcaggaggaa tattatttgc tttatcagta actctaagtg gacaagctgc
    42481 tgttcctgaa acaggctggt tggctaaact gaatcaaatt ggagctgctg gcttggcttt
    42541 attcattcct atattaggtg gatatatagc atttagtatg gcagataaac caggattagc
    42601 tccaggtatg ataggtgcat atttagcaaa agaagtaaat gcaggtttta taggtggtat
    42661 catagcagga tttatagcag gatttgttgt tttacaatta aagaaaatta aattagctcc
    42721 tacaatgaga actttaggtt ctatatttat ttatcctttg attggtacat tgattacagg
    42781 tggtataatt gtatttttaa ttggagaacc tatagcttct tttatgacat ggatgacaaa
    42841 ctggttaaat ggaatgtctg gtgtttctaa aattccttta ggtggaatct taggaggaat
    42901 gatagcctca gatatgggtg gtcctataaa taaggttgct gctacttttg cccaaactca
    42961 agttgacaca cttccatatt taatgggtgg agttggcgtt gcaatttgta tacctccaat
    43021 tggtttagga ttagctacat tattgtttcc aaaaaaattt agcaaggaag aaagagattc
    43081 aggtaaagct tcgctattaa tgggatgtgt tggaattaca gaaggagcta ttccatttgc
    43141 aacatcagat ccagttagag taataccttg tattatggct gggtcgatta taggaaatat
    43201 tatggctttt ttattaggat gtttaaatca tgcaccctgg ggaggtctga tagtattacc
    43261 tgtagttgac aataggttgg ggtatattgc atcagtatta actggagcag tagttgttgc
    43321 agtattaatg aaattagtaa aaaaagacgt gaaagaagat gaagaaattg aagaggattt
    43381 agatgaatca attgaattaa tatttgaaga attataaata taaatggaag gaatttgtat
    43441 aaaaaagtaa atataaaact ataaacttaa aggagataag aatatgaaaa ttgtagcagt
    43501 aacatcttgt cctagtggag tagcacatac ttacatgtcg gctgaatcac ttgaattatc
    43561 ggccaaaaaa tttggtatag aaataaaggt tgaaactcaa ggaagctctg gaatagataa
    43621 tgaattgtca cttaaagata ttgatgaagc aacttgtgta atattgacta atgatgttga
    43681 gattagaaat atggaacgtt ttaagggaaa gaaggtagta agaatgagtg tatctgagat
    43741 aattaagaaa tctgatgcat taattaagaa aataaaagat acttttcaat aaaaattgat
    43801 ttattttaca tataactcaa agatgttttt aaagaaattt ttattaaaag catctttgaa
    43861 aatatcttaa aaatagaata aaaggagata tagtcatgaa aaaattacca ataaagactg
    43921 tcgtacaaag attactagac ttacaagaag aggggaaaag tgcaactctt ttaggaattg
    43981 gtcctatgtc accaaacttg ttacaagcaa gttttgaact agcaaaagat gatgattttc
    44041 ctttaatgtt tatcgcaagt agaaatcaag tagatgcaga tgaactaggt ggcggttatg
    44101 taaatggttg gaatcaggaa acttttacaa aagatattaa gaagattgcc gacaaagttg
    44161 gatttgatgg tttatattat ttatgtagag accatggtgg gccatggcaa agagataaag
    44221 agagaaatga ccatttgtct gttgatgaag caatggagtt aggaaaaaaa tcttatttag
    44281 cggacattga agcaggtttt gacctattga tgattgaccc tacaaaagac ccatttgaaa
    44341 ttggaaaagt aattccacta gatgtagtac tagaaagaac tgtagaatta attgagtatt
    44401 gtgaaaacga gagaaaaaga cttaatctac cagacatagg ttatgaggtt ggtacagagg
    44461 aaaccaatgg aggtcttact tcaactgaga cgtatgaaac atttataact agactaaagg
    44521 ttgaacttga taataggggt ttgccaatgc caacatttat agttggtcaa actggtactt
    44581 taactagaaa gacagaacaa gtaggaactt ttaattttag aaatgcttat gatttagctc
    44641 aaatggctaa gaaatatgga gttggtttaa aagagcataa tggcgattac ttagatgatg
    44701 taactttatt agaacatata ccatctcaaa ttattgctac aaatgtagct cctcaatatg
    44761 gtactgaaga aactagggca tatttaaaat tagcagaagt tgagtgtaaa ttagaaaaag
    44821 agggcctagt tgataaaact tctaatatca gacatgttct tcttgttaat gcaattgaat
    44881 gtggacgttg gagaaaatgg gtagtaggag aacaaaaaaa tctaactact gaagagatat
    44941 ttaagaatga agttttatct aatgaaatac tagatattgc aggacattat acatttaata
    45001 atgatgatgt taaaaaagaa atagaagtgc tttatgataa tttaagtaag aataatattg
    45061 atggtcaaag atttgttgta gaccatatta aacgtccttt gcgtgattat gcagaatgct
    45121 ataacttaaa aggtgttact acgaggattt taaataaata ataggactta tagtggtgag
    45181 tattgttata ctaggtattt atagttataa ttatacttac cactattaaa tttaggagga
    45241 ctaatatttt gattaattta atttttgatg tagatgatac tttatataac caattaacgc
    45301 ctttttacac tgcatataat aaagtgtttt cttcaataaa ggatatttct attgaagatt
    45361 tatatatgag tagtagaaag tatagtgatg aggtatttca tatgactgaa aatggagaaa
    45421 tgccaatcaa agaaatgcat atttatagga taatgaaagc ttttgaggag ttgggaaatt
    45481 ctattacaga aaaagatgca caaagttttc aagatgagta tatatatcag caatcacaaa
    45541 ttacactaat accagaagtt gagaggattt taaatttttc taaagaaaga aatattaatc
    45601 taggaattat aactaatggc ccatcaaacc atcaaagaat gaagttaaag cagttaaata
    45661 ttgaaaattg ggttgataaa agtaatattt ttatttctag tgaggttgga ttttctaagc
    45721 cagatactaa tatttttaga gtagctgaga atgttatgaa tttagataga gaaaatacat
    45781 attatgtggg tgattcttat agaaatgatg tattaggtgc taaaaaagct ggttggaaga
    45841 gtatatggtt gaaccataga ggacatgagg tagaagagtt gttttataaa cctgattttg
    45901 taattttgga acataaggat ttgatatctt tatttataaa aatatgtagt tataaaaata
    45961 tgtagtaaaa aaatgttaat aatagaattg tataaaataa ttaataataa ctatataaaa
    46021 ttatatgttt agattgtgag caatctattt tatgttaaaa atagattatt tagaagaagg
    46081 ggaaatctta aaaaaatttg aatatcccct ttttattatt taattaaaat gaaatagtta
    46141 gaaatatatt ttatttagtt tagtcaataa cattttggga ttctttataa ctacatgtta
    46201 actgttgaat aggcttatca tatggtttaa cccatccttt attaatactt aattctgtaa
    46261 gtgctgtgtg accttctacc atttgagtta gtgaagctga atatatagct cttaattcag
    46321 gtgtactact tgttaatgca gaattaagat acatattggc agatgcttta gcagatgaaa
    46381 gcatacttaa cattattact ttatcgtcta tataagtatt gttcttaatt agattttcta
    46441 tcatatttct catgttatct atacctcctc agaaattagt atttcatttt caactataaa
    46501 ttgttgtata gctttaattc ttccttctgt tgctaatata ctagattctg attgtctttt
    46561 taggtcttca tctgatatta acatatttat tgctctttgc ataagtaatc cgtcagactc
    46621 catttttata actgctgcta aagatatttt ttcagtttct gaaagtgttc tcatttaatc
    46681 atctcctata ttgtaattta tgctttaata gtatgtcttt cttttcaagg aaataaccat
    46741 tacacttttt tacagtattt tatattctct ttatatcaaa gtttagtcaa cttaatatat
    46801 taatataatt ttgtaggata tttatcatta aatatctata aaataaatta tttgaatatt
    46861 cataaatcaa aatttatttg tatgtacttc ttcaagagtc aatatgaatt ttttcaaaaa
    46921 attaattttg atgataatca ttaagggcta tatttttcct aaagttgtga ttatcattat
    46981 taatttacta aaatacttta atgaaaaata gctaacttta attgtaggat attcttaata
    47041 tatttgaaat aaataaaaat attgaaggat ataatattca attggtatat aaataataaa
    47101 tgatagttaa gatttacctt agaggctaat taggtgttca gtcaaaatta atgcctttag
    47161 aatacaaatg tattctaaag gcatttttac tatgtatttt catttatatt actaaacatt
    47221 atctattttt atatacattt ctatgtattt tattttcatc aaatcggtct ataaattcat
    47281 ttttttcttc actatatcca atagatataa gtgcataagg aactaatttt actggtaagt
    47341 ctaaaatttg tttaacttta tttactactt tatttttagg aaatactcct gcccaacaag
    47401 cagctagacc ttgattagta acttctaata ataaattttg tgtgcaagca cctaaatctt
    47461 gtatccattt accaggtttt aagaatttat ctctatttcc aagcactact atacataatg
    47521 gtgcaaattc tatatgcttt gctctatgct gtgactttga gagctctttc agcagttcct
    47581 tatcatctac tataataaat tcccaaggtt gagcattttt tgatgaaggt gcttgcatag
    47641 ctgattttag tatttcagtt attttatctt cttctatatt tttatctata tatgttctta
    47701 ttgaacgtct attttttata acttctaaca tacagattct cctttaaata aaattatttc
    47761 tatttatata atatcattac tttaagtttt agctattact tatataaaga ataaactttg
    47821 tgttggtttt atctaagtaa aagtaacatt cagaactaaa aaacaagact ataaatccaa
    47881 gatattaatt aaaaaagaag taataaaaaa taatatacaa gatttaaaaa agagtattga
    47941 gcttaaaatt taattagaaa accaattttg agataatctg ttttttattt taacaaaaaa
    48001 acataaataa agttcgatac gtagcgaatg aaaaaaatac aagaaatatt ttttttaaaa
    48061 aatttcaaaa aagagttgat aatatatgta tattaatata aaatttaagg tgtataattc
    48121 gtataataat acgaatgatt tgtagtgtaa aataacttat aaacggagag gggaagtcta
    48181 atgaaagtag cagtagttat gggttcaaaa tcagattatc caaaattaga agaagggata
    48241 aaactgcttg aaaaatatgg aatagaggtt gtagcaagag cattatcagc tcatagaaca
    48301 ccagaacaac tttcaatatt cctaaaagaa atagaagatg atactgatgt aataatagca
    48361 gcagcaggaa aagcagcaca cttaccaggt gtaatagctt cacaaacatt aattcctgta
    48421 ataggattac ctattaaatc atcaactatg gatggactag actcactttt atcaatagtt
    48481 caaatgccaa aaggaatccc agttgcaaca gttacaatag acttaggttt aaacgcagct
    48541 ttattggcat tacaaataat gactttaaaa tacccaaaat taaaagagga tttgaaatca
    48601 tatagagaag agatggcaca aaaggtgtta gaagacgaca agaatttaag ggggtagcag
    48661 aaatgttatt atatgaagga aaagcaaaac aagtatattc tacagataat gaaaatgaat
    48721 atgttgttta ttataaagat gatgcaacag catttaatgg agaaaaaaaa gcagaaatat
    48781 cttcaaaagg tatactaaat aataagatat caacaataat ctttgaaatg ttaaaagaaa
    48841 ataatataaa tactcacttt ataaaaagtt tatcagatag agaaatgtta gttaaaaaag
    48901 tagagatttt accattagaa gtaatagtaa gaaatattgc tgctggttct atctgtaaaa
    48961 gagttggtct tgaagaaggt gtagttttcg atgagccaat ttttgaaata agttacaaaa
    49021 atgatgctta tggtgaccca atgctaaatg atgattatgc agtggcaatg aagttagcaa
    49081 ctagagaaga gttaaaattc ttaagagaag aaactttaaa aataaacgag ttactaaaag
    49141 catttttctt aaaattaaac ctaaagttag ttgactttaa aatagaattt ggtaaggatt
    49201 ctgaaggaaa tataatattg gcagatgaag tatcaccaga cacttgtaga ctttgggatg
    49261 taaatacaaa tgaaaagtta gataaggaca gatttagaaa agaccttgga gaccttgtag
    49321 aaggttatac ggaagtactt tcaagaatga ataataaata ggaggcaaat gtatgtgtgg
    49381 agttttaggg atttactcta ataaagatgt aactaaagaa ctatattatt ctttatattc
    49441 tatgcaacac agggggcaag aaagctgtgg tttagctctt ttagatgatg gagaaattaa
    49501 gtacaaaaaa gatatgggat tagttggaga tgtttttaag gaaaatgaat tatctaagtt
    49561 aaaaggaaat atagggattg gacatgttag atattcaaca gctggaggaa gtcatgtttc
    49621 taactgtcaa cctttagttg gaagttgtag aaaaagacaa ttagccatag ctcataatgg
    49681 aaatctagtt aatgcaaatt atttaaaaga tatgctagaa gaagatggat atatgttcca
    49741 aactaattca gatacagaag tgattttata tatacttgct agatattaca aaggggatat
    49801 agtagaaagt ttaaaagtga caatggatta tataaaaggt gcatatgctc ttgtaataat
    49861 gagtcaagaa gaattggttg cagttagaga cccacatgga ttcagacctc ttgtacttgg
    49921 taaaaagggt gatgaatata tatttgcatc agaaaattgt gcaatagata tacttggcgg
    49981 agaagttata agagatgtag agccaggaga gataatagta gtcaaagatg gagaactaaa
    50041 atcatatttt tattcagaaa attataaacc agttaagaaa agttgtatat ttgaacacat
    50101 atactttgca agaaatgatg caactataga caatgtaaat gcatatgaat ttagaattaa
    50161 atgtggtgag agattagctc agaatgaaac tgtaaaggca gatatggtag ttccagttcc
    50221 agattcaggt tggccaggag ctattggtta tgcaaatgct agtggattaa aaataagtga
    50281 aggcttagtt aaaaatagat atgtaggaag aacatttata aaacctacac aagaagaaag
    50341 agaaatagca gtaaaaataa aattaaatcc tctttcaaca ataataaaag gaaaatctat
    50401 aattttagta gacgactcaa ttgtaagagg aactacatct aaacaattag taaaatcttt
    50461 aagagaagca ggagcaaaag aaattcatct tagaataact tctcctccag tagcttactc
    50521 ttgttactat ggaatagata ctccaaatcg ctctaaatta attgcatcaa gtaacaacgt
    50581 agaagaaatg agagaatata ttggatgtga tagtttaaaa ttcctagata tagaaggaat
    50641 gttagatgca acagaacata agtctacttt ctgtaaagct tgttttgatg gagaataccc
    50701 agttaagaaa attgataagg aggaactact ttcatgttaa catataaaga atcaggtgta
    50761 gatatagatg aaggaaatag agcagtagac cttataaaag gtaaaataaa aggaacttat
    50821 gatggaaatg tagttggaga tttaggtaac ttcagtggat tatacagttt aaaagatttt
    50881 gtaggtatga aagaaccagt tttgcttgca tctacagatg gtgttgggac taagttaaaa
    50941 atagctcaga tgatggacaa gcatgatact gttggaatag atttagttgc aatgtgcgtg
    51001 aatgatttaa tttgtcaagg agcaaaacca ttatttttcc ttgactatat agctcttggg
    51061 aaattagtgc ctgagcatat cgaaaaaata gttggtggaa ttgcagatgg ttgtaaaatg
    51121 tctggttgtg cactaattgg tggagaaact gctgaaatgc ctggaatgta tggagaagat
    51181 gactatgatt tagcaggatt ttctgtagga atagcagaca aagaaaagat tgtttctgga
    51241 aacaatgtta aatcaggaga tgtattggtt ggtatatctt caagtggagt acacagcaat
    51301 ggattttctt ttataagaaa gatattttta gaaacttaca attataaaat ggaacaatat
    51361 gttgaagaac taggaatgac agttggagaa gcacttttaa caccaactaa aatatatgtt
    51421 aaattagctt tagatgtatt agctaaacat gacataaaag ctatagctca cataactggt
    51481 ggagggctta tagaaaatat aacaagagtt atacctaaag ggttaggact agatatcaat
    51541 aaaaaatctt gggagaagcc tcctatattt aaaatgatag aaggttttaa tgctgttgat
    51601 gagagagaac ttcataagag ttttaacatg ggtattgggc tagttttaat agtagataaa
    51661 gaaaatgctg atgatgttgt aaactttata aataatagag aaaatgacaa tgctgcttat
    51721 gtagataaaa aatacagtga attattagaa gataaagcgt atataattgg tgaagtagta
    51781 gatagtcatg aaggtgtaga attatgctaa atataggagt tttaatatct ggtggaggaa
    51841 caaatttaca agcagttata gatggcactg agtctggaga aataaaaggt caggttaaag
    51901 tagttatctc aagtaagcaa ggtgcttatg ggctagagag agctaaaaat cataacataa
    51961 aagcaatttg tgaaactgat gaagacaaga taatagagat tcttaaggaa aataaaatag
    52021 atttagtagt tttggctggt tatttaaaaa taatcagccc aaagctggtt aatgagttta
    52081 gaaacaaaat gataaacata catccatcat taattccttc attttgtgga gctgggttct
    52141 atggagaaaa agttcatcaa ggtgttatag attacggagc taaagtaact ggtgctacag
    52201 ttcattttgt tgatgaaggt gctgatactg gaccaatcat aatgcaagat gtagttaagg
    52261 taaatcaaga tgatgatgca aagacacttg caaaaagagt gttagaagtt gaacatagga
    52321 ttttaaaaga aagtataagt cttttttgtg aaaataaatt aaaacttcaa ggtaggagag
    52381 tgtttataaa tgagtaagag agctttaata agtgtaactg acaagactgg tgtagtggaa
    52441 tttgctaagg agttaaataa gttagattat gaaattatat ctacaggaaa tacatttaaa
    52501 acattaaaag aaaatggtgt taatgtaatg caagttgaag atgttacaaa ttttccagaa
    52561 atattagatg ggagagtaaa aacattaaat ccatatatac atggtggtat actttataaa
    52621 agagataaag aatctcatgt agaaactgtg aatgagcata aaatacattc aatagattta
    52681 gtagctgtaa atctatatga ttttgaagga actttaaaag ctggaaaaag tcatgatgaa
    52741 ataattgaaa atatagatat tggtggacca tctatgatac gttctgctgc caaaaactat
    52801 aaagatgtaa tagtagtagt agatataaaa gattatgatt ctataataga aaaattaaaa
    52861 acagatacaa tgacattaga agacagaaaa aaattatcat ataaagcttt ctcaactaca
    52921 ggaagatatg atgcgttaat atctagttat tttgctggag aagttggaga tacatatcct
    52981 gacatattaa atttaacttt ccaaaaagaa caaactttaa gatatggaga aaatccacat
    53041 caaaatggtt tcttatattc acaatcaaat gcaaaaaatc caatcttaaa ttatgagcaa
    53101 ttaggcggta aagaactttc ttttaacaat ttaaatgatt tacatggatg tttagaagta
    53161 atgagagaat ttaaagatag tgaagaagta gtttcagtag ctataaaaca tgctaattca
    53221 tgtggggtag gtctaggaaa agatgctttt gaagcatata caaaatgtta tgaagcggat
    53281 aaggtttcaa tatttggagg aatagtagga ataacttcta ctatagataa agctacagca
    53341 gagaaattaa atgaaatatt cttagagata gtagtagcat atgattttga gccagaagct
    53401 ttagaggtac ttaaacaaaa gaaaaattta agaatattaa aattagctaa gatagaagat
    53461 agtttacaac catatgaaat gaaatattta gatggaaaat tattaataca agatagaaat
    53521 aatatattag cagaaaaaag tgaaaatgta acaaaagcaa aaccaacaga tgcacaactt
    53581 aaggacatgg aatttggtat gagagtagta aaaaatatga aatcaaatgc aattgcgata
    53641 gtaaaaaatg gtcaaacttt agctttaggt tgtggacaaa catcaagaat ttgggcatta
    53701 aaaaatgctc ttgaaaataa caaagataaa gattttacag gagctgtttt agcgtcagat
    53761 gcatttttcc catttgatga ttgtgtaaca ttagctcatg aatatggaat atcagcagta
    53821 gttcaaccag gtggctctat aaaggataaa gattcaatag aagcttgtga taagtatgat
    53881 atggttatgg tatttacagg aataagacac ttcaaacact aaggaggata ttatgaagat
    53941 attggttgta ggcggtggcg gaagagagca tgctatatgc tggaaactaa gcaaagaaaa
    54001 aaatgtagaa aaagtttatt gcgcgccagg taatgcagga attacaaatg tagcagaatg
    54061 tgtaaatata ggtgatacta atatagaaga acttttaaaa tttgccaaag aaaatgaaat
    54121 aggtctgaca atagtaggac cagaagtacc actagttatg ggaatagtag atgagtttga
    54181 aaaagaagga ttaagagtat ttggacctaa taaaaaatgt gctcaacttg aaggaagtaa
    54241 agctttttca aaagaattta tgataaaaca taacatacct actgcaaaat ataaagaata
    54301 tacaaactta gaagaagcta tatcagaaat agatagtttt ggatatccag tagtaataaa
    54361 agcggacgga ttagctgctg gaaaaggtgt tgtaatacct gaaaatagag aagatgctat
    54421 agcaacttta aaagaaatga tgtcagataa gaagtttgga gctgctggag ataaaatagt
    54481 aattgaagag tttttaaaag gaatagaaac ttcaattcta gcatttgtag acaatgatac
    54541 aatagttcct atggcaagtg caaaagacca taaaaaagta aacaactatg agcaaggtcc
    54601 aaacactggg ggaatgggaa ctttttcacc tagtgaaata tatacagaag aattagcaaa
    54661 taaagtaaaa gagacagtat tagaaaaaac tttagaaggc ttcaaaaaag atggacttaa
    54721 ttttaaaggt attttatttg ttggtcttat gataactgaa gatggagaaa aagtacttga
    54781 atataatgta agatttggag accctgagac acaatctgtt ttatttagat tagaaacaga
    54841 tttacatgag ataatggaaa ctatactaga taataagtta aaagatatag aaataaacta
    54901 tagtgatgat gaagctgtat gtgttatgct tacatcagga gggtatccag acagttatga
    54961 aaaagggaaa attataactg gtcttgaaaa cttggatgat gatatagtag tattccatag
    55021 tggcactaag atgcttgatg gtaatttagt taccaatgga ggaagagtaa ttggtataac
    55081 tgcaaagtca actacagtta aggatgcagc agaaaaagtt tatgaaaaca taaagaagat
    55141 aaactttgaa ggaatgcact atagaacaga cataggaaga taatttttct aaaatggagg
    55201 ataaggtatg ttgaatactg aaaataaaga ctcaatggtt agaagagtat tagttgaaaa
    55261 aagagaaggt tttgaccttg aggctaaagc tttaaaaaaa gacttagtag aaagtttaca
    55321 catagataat atagaaaatt taagaatatt aaatagatat gatgtagaag gaatatcaga
    55381 agaagtttat gaaaatgcag caaagacaat attctctgaa ccaaatttag atgtagtata
    55441 ttatgaagaa ataccaaaat taaatgatga aagagtattt gcaatagaat ttcttcctgg
    55501 tcaatacgac caaagaggtg attgggcagc tcaatgtgta caaattgtaa atcaagggat
    55561 aagacctgct attaatacag caaaagttta tatattatca ggcaaaataa ctgatgaaga
    55621 gttttctaaa ataaaagatt actgtataaa tccagtagat agtagagaag catctttaga
    55681 aaaaccagaa acattaaaaa tggaaactga aataccaaca acagttgaag tattagatgg
    55741 atttatagat ttagatgaaa atggattaag aacatttgta agtgaaaaag gattagcaat
    55801 gactttagga gacttacaac acgttcaaaa atactttaaa gatacagaaa agagaaatcc
    55861 aactataaca gaaataaaag tattagatac ttattggtct gatcattgta gacatactac
    55921 ttttatgact gaaatagaaa atgtaaagat agaagatggt aagtttaatg atatagtaaa
    55981 agaagcgtat caaatgtatc ttaactcaag agacaatgta tatgtgaaca gacataaaga
    56041 tatatgttta atggatatag ccactgttgc agtaaaagaa ttaaagaaga atgggaaatt
    56101 aaatgaccta gatgaaagtg aagaaataaa tgcatgtagt ataaacgtag atgttgaagt
    56161 tgatggtaag atggaaaaat atctagtaat gtttaaaaat gaaactcaca accatccaac
    56221 tgaaatagaa ccatttggtg gagcagctac ttgtttaggt ggagctataa gagacccatt
    56281 atcaggaaga agttatgttt atcaagcaat gcgtgttaca ggaagtgctg acccaagaac
    56341 gactttagaa gatactttac ctggaaaact tatgcaaaga aaaataacta cagaagctgc
    56401 tcatggatat agttcatatg gtaaccaaat aggtcttaca actggacaag ttgcagaagt
    56461 atatgatgaa aactttgttg caaagagaat ggaaataggt gcagttatag cagcagtacc
    56521 aaaagagaat gtagtaagag aaagacctga agcaggagat gtaatagttc ttcttggagg
    56581 aaaaacagga agagatggtt gtggaggggc aactggttca tctaaagaac atagtgaaga
    56641 gtcaatctta acttgtagtg cagaagttca aaaaggagat gctcctaacg aaagaaaaat
    56701 acaaagattc tttagaaata aagaagttgc tcaaatgata aaaagatgta atgattttgg
    56761 agcaggtgga gtttgtgttg ctataggaga gattgctgat agtttagata taaacttaga
    56821 cttagtacct aaaaaatacg atggacttga tggaactgag cttgcaattt cagagtcaca
    56881 agagcgtatg gcagtagcta taaaaaaaga aaataaagat aaatttatac aattagcagt
    56941 agaagaaaac ttagaagcta cacatgtagc tacagtaaca gatacaggat atttaagaat
    57001 gttctggaat ggaaaagcaa ttgtagatat aaatagagag ttcttagata caaacggagt
    57061 aaaacaaact actgatgtcc atgttactaa agtagatgaa gaaaatacat tctttagctc
    57121 taatgaaata gtaaaagatg ttaagtgtgc aagtatgaaa gacaagttta caaaagtatt
    57181 atctgacctt aatgtttgtt ctcaaaaagg attagtagag atgtttgata atacaattgg
    57241 tggtaataca gtacttatgc catttggagg aaagtatcaa gcgactccaa cacaaggtat
    57301 ggttgctaag ataccagtac ttggaggaga aactaatact tctacaataa tgacttatgg
    57361 atataatcca aaggtaggaa aatggagtcc attccatggt gccttgtacg cagtagtaga
    57421 atctgtgtgt aaattggttg ctattggagg aaattatagt acaacaagac ttacattcca
    57481 agagtatttt gaaaaacttg gtaacaatcc agaaaaatgg ggtaaaccat tctcagcact
    57541 tttaggagca ttttatgcac aaagtaaatt tgagatacct gctataggtg gaaaagacag
    57601 tatgtctggt acatttaaag atatcgaagt tccaccaaca ttagtttcat ttgctgttga
    57661 tacagtagat gcgaagaaag tagtatctcc agaatttaaa aaagcagact caaaagtagt
    57721 aatgttatgt gtaaacaagg cagaaaatga tgttgtagat tttgaggaat taaagagaaa
    57781 cttagacaaa gtaagagaac ttatacatgg aaataaagtt ttatcaacat atgcattagg
    57841 atttgcaggt gtaggtgaag caataagtaa aatggctttt ggtaataaaa taggatttaa
    57901 gtttagtgaa gaagctgaaa aaacatttac agatgataaa ttatttgagg ctagttatgg
    57961 aaatatagta cttgaattag caaatgatga tttatgtatg ttagaaggat acaattatgt
    58021 tgtgcttgga agtacaacta aagaagctag tatatttatc aaaggtgaag aacttgcatt
    58081 agatgaatta tataaagcac actgtagtac tttagagcct atattcccaa ctaaaacaga
    58141 agaagtaaaa tcaaagatag agactataag ttatatatca caaggagaag ctaaaaaatc
    58201 atcattaagt atagctactc caagagtatt tataccagca tttcctggaa caaactgtga
    58261 atatgactct gcaagagcat ttgaaagagt aggagctaat gcaaacataa gagtattcaa
    58321 gaatttaact tataaagaca tagaagattc tatagataca atagtaaacg aaataaaatc
    58381 ttctcaaata ataatgttac caggtggatt tagtgctggt gatgagccag atggttctgg
    58441 taaatttata gctactgtat ttagaaatcc tagagtgcaa gaagctataa atgaattttt
    58501 aactcaaaaa gatggactaa tgttaggtat ctgtaatgga ttccaagtat taattaaatt
    58561 aggattagtt ccatatggag aaataagagt tccaagtgaa agtgctccaa cacttactta
    58621 caataatatt ggtcgtcatc aagctaagat tgctagaact agaatttcat ctaataaatc
    58681 tccttggtta gctcaaacta atgttggaga tattcataat atagcaattt ctcatggaga
    58741 aggtaaattt gttgctagtg aagatgttat gagagaatta attgctaatg gacaagtagc
    58801 aactcaatat gttgacttta ataatgaagc tacttatgat attgagttta atccaaatgg
    58861 ttcattctat gctgttgaag gtataactag tgctgatggt agagtgtttg gtaaaatggg
    58921 acattctgag agaattggtg aggaagtatt taagaatatt ataggtgaaa aagaccaaaa
    58981 gatatttgaa tctggagtta aatattttag ataaagcttt gatgtttaat tgatagctag
    59041 aatatataat ttagatattt agatttaaag agatgtaata atatgatgat aatcatttaa
    59101 tattacatct cttttttatt tatctaagaa ataataagtt caacctaagt taaaatacaa
    59161 gctgtgttat aattaattac taagtaaaat taaatattta ttggagggca acataattaa
    59221 tgaaaatatt aataacaggg tctaatggtc aattaggaaa agaattggtt agccaattag
    59281 aagcaatcaa tcaaagtata aatcaaccaa aatacgttat attagctaca acaagaaatg
    59341 atttggatat atccaatcag actaatatag ataattttat tttgcataat aaacctgatg
    59401 taattgtaaa ctgtgcagca tatacaaagg ttgatgcatg tgaagataat atcgaaattg
    59461 cgtataaaat aaatgcctta ggagtgagaa atttagcaat agcatcagaa aaagttaatg
    59521 ctaagcttgt tcacatatca actgactatg tattcaatgg tttttctaaa tatccatata
    59581 gggaagacaa taaaactgaa cccaattctg tgtatggaaa aagtaaactt atgggagaaa
    59641 agtttgttga acaattctca cataagtact ttatattgag aacagcatgg ttatatggta
    59701 atgggaataa ttttgtaaag actatgataa aactttcttt agaaaataaa gaggttaatg
    59761 tggttaatga ccagtttgga accccaacaa gtacagtaga cttagcaaaa attataataa
    59821 gaatcatgga aacagaacat tatggagttt atcatgctac atgtgaaggt gagtgtagtt
    59881 ggtatgattt tgccaaaaag atatttgaat taaaaaatat agatataaaa gtaaatccaa
    59941 ttaagagtaa cgaatttaaa tgtaaggctc aaagaccact atattctgta ttggataatt
    60001 ttatgttgaa gttaattggt ttaaattctt ttagaaagtg ggaagaatcc attgaagaat
    60061 atttagaaag atatgatata aatgaaaagt aaatgtatta tttttagaaa agaaaataag
    60121 atattataaa aaatataaat atttctttta tttatccgat aatcataata tgacaaaaac
    60181 aaatttaagg atggaaaaag atgaaaggaa ttattttagc aggagggtct ggtacaagac
    60241 tatatcctat aacaaaatgt atatcaaaac aaacacttcc aatatacgat aaaccaatga
    60301 tatattaccc tatgtcggtt cttatgttgg cagggataca agatatatta ataatatcta
    60361 caccaagaga tataaaagca tttgaagaat tatttggaga tggaagttca ctagggctta
    60421 aaataacata tgcaattcag gagaaagcaa atggaattgc agaagcgttt ataataggag
    60481 aagaatttat aggaaatgac agagtgtctc ttgtattggg ggataatata ttttatggat
    60541 atggatttag tgaaaggttg gagaatgcag ttaaaagaaa aggagcaact atatttgggt
    60601 atcatgtaag taatccaaat tcgtttggag ttgtagaatt tgataaggat tttaatgtat
    60661 tatcaataga agaaaaacct acagttccta aatctaatta tgctatacca ggattatatt
    60721 tttatgataa tgatgttgta gagatagcaa agctagttga accatcagat agaggtgaat
    60781 tagaaataac ctctataaac agtgaatatc taaaacgagg aaaactaaaa gtggaacttc
    60841 taggaagagg tatggcttgg cttgatactg gtacacataa aggtctattg gatgcttcta
    60901 actatgttga agtggttcaa acaagacagg gactgtatat agcatgttta gaagagattg
    60961 catatacaaa aggatatata agtagagaac aattaatgaa actagccaag ccactgataa
    61021 aaactgaata tggtcaatat ctattaaaat tggctagtga agatatgcaa tatggtagat
    61081 aaaatttatt ataaatttta ttaagggagt ttgttataat gggaaaaatc aatgttataa
    61141 agactaaaat agaaggatta tatatagtcg aaccaaatgt attttgtgat gaaagaggat
    61201 attttatgga aagctataat aaaaaggatt ttatagagca gggtcttgat atgtgtttta
    61261 ttcaagataa tgagtctagc tcaagaaaag gtgttttaag aggaatgcat tttcaggtta
    61321 gatatcctca aggaaaatta gtcagagtta cagagggaga agttttcgat gttgcagttg
    61381 atttaagaaa agattcacag acatttggga aatgggaagg tgtaatattg tctgataaga
    61441 ataaaaaaca attttatatt ccagaagggt ttgcacatgg atttttagtt ttatcagaaa
    61501 aagcagtatt taattacaaa tgtacaaact attattatcc agaatttgag catgggttta
    61561 tatggaatga caagcaaata tctattgagt ggcctttaaa aggaattgaa gaaatatttt
    61621 tatcagaaaa ggacaaaaaa caaaaaagtt ttaaagatat ttgtataaga cgttaataaa
    61681 aatttattag aattaacatt taggagaaaa aatatgaaaa aaatactgat aactggagga
    61741 gcaggattta ttgggagtaa ctttgtacat tatatgctta acaagtatgt agattatttt
    61801 attgttaacc tagacttgtt gacttatgct ggtaatttag agacattaat agaaatacaa
    61861 gacagttcca attataaatt tgttaaagga gatatttcaa atagggaaca gatgtataag
    61921 ctatttgaag aagaaaggtt tgatatagtt gttaactttg ccgctgaatc tcatgtagat
    61981 aggtcagttg agaatcctga tttatttatt aaaacaaaca ttataggaac acaagtactt
    62041 ttagatgcat ctataaaata tggagttaaa aggtatcatc aaatatctac agacgaagtg
    62101 tatggtgatt taccaattga tagaaaggat ttattcttta cagaacaatc tcctatcaat
    62161 ccaagttctc catattcagc ttctaaagca tcagcagatt tgttggtaag ttcttattat
    62221 agaacatatg gattgttaac aactatatct aggtgttcca acaattatgg gccatatcat
    62281 tttcctgaaa aattgatacc tcttatgatt ttaaatgctt tggaaaacaa acaattgcca
    62341 gtatatggga atggggaaaa tgtaagagat tggttacatg tatatgacca ttgtactgct
    62401 atagacttaa taatacataa agggaatata ggggagattt acaatatagg aggtcataat
    62461 gaaagaagta atttagaagt agtaaaaatg atattaaatc ttttagataa atctgaagag
    62521 ttaatatcct atgtaaatga tagacctggt catgatttaa gatatgctat tgatgcaact
    62581 aaaatagaga atgagttagg ttggaaggca aaatatgact ttgatttggg tataaaagaa
    62641 actgttaagt ggtatataga aaatgaatct tggtggaaag ctgttttatc agtgtaactt
    62701 tgaaatttat aaaatttaga aaggggcgtt ttatattgaa tgagataagt tcactaatta
    62761 atatattagc attgactcaa ttatctggta gtaatactac aaatcaatgt aactgtacat
    62821 ataatagttc atctggattt gatatgatta tgatgacctt gttaaaagca ttatcacaaa
    62881 ataatcaaca gacttctaat ggctacaatt tatcgtctaa ttctgagaat ttatttgacg
    62941 aactcggcag tgtagtaaat aatacagcta gtagatttat agatgttgat acaaaagata
    63001 aaagtgtaaa atcaagaatt gaaaatgcta tagagcaagc atcaaaaaaa tataatgtgg
    63061 attcgaatct tataaaagca ataattaaag ttgaatcgga ttttaatcca aatactgttt
    63121 cttctgctgg tgctaagggt cttatgcaac ttatgccaga aaattgcaga gacttggggg
    63181 ttacagaccc ttttaatata gagcagaata tagatgctgg aactagacat attaaggaat
    63241 acattgatat gtttggaggg agcatagaaa tgggattgat ggcatataat ggtggtcctg
    63301 gaagaatgaa aagtagagga gtagaatcaa tctctgacct gtataaaatg ccaaaagaaa
    63361 cacaaaatta tattcctaag gttatgaaat attatagagg gtaggcttta ttatggggat
    63421 aaatatagat aagctaaaca taagtccaag tataaatatg gaggttaaaa aactaaaaga
    63481 gagtaataaa ttttctgaca aattaaactc tttaaaacaa tcttcttcat tggaagatat
    63541 aaaacgaaat ttggaaagtg taaaaaaggc aggagaaagg cttgtactta ctcaaaacta
    63601 tggcgacatt agtatatata aaaatgcagt aaaagaatac ctaaaatctg ttgtagataa
    63661 tatgtattca cttgataaaa atagtagctt ttgggagcat cagtattata agaatgtaaa
    63721 gattgttgat agtaaacttg aggatatgac tagtaagtta attagtgaag aaaaagaaaa
    63781 catagatatt gtttctactg ttgatatgat acaaggcttg ttgatagata tgtatagata
    63841 aggttataca aggagaaaag ttttatgaat agtaaaaaag aggatatata tgaattgaat
    63901 tttgggcaat taatagaaga aaaatctggt gttatagaag aaaatcaaaa tgtttttaat
    63961 gcaaaagtaa atgtagctgt ttctatagga aatacaacag aaacaataaa aaatatatta
    64021 gatttaaaag aaggctctat aataaaacta agtaagaatg tggatgaaaa attagatata
    64081 tatgcaaatg atagattgtt tgcttatgga gaaagcatta ttacaaatga gaagttatct
    64141 gtaagattat ctaaaataga agattcagat gtaaattagt attttaaata gtatcccttt
    64201 aggctatgat gtatgagttt agctaagggg atatttttat aaaaaattta aaatatgcgt
    64261 gaattatata aatgttttta ttttatgagc gataattaaa ttataagtat gtaattttgg
    64321 aggtgaaact atggatatta aaaatgtaag ttcaaatatt gtagtgaata attatttaaa
    64381 gaatacatct ggtgtaaaaa aagtggaaga agttaaaaaa attaataaaa cagacaagat
    64441 agagctatct aaatttgcaa aggaaatggc aagtgtgtca aatatgagtg atgagaatat
    64501 taggaataaa aaagtcgatg aaataaaaaa tgcattagaa aatggaactt acaaaataaa
    64561 accaaaagaa attgcgaaaa aaatgataga ggatatgcga ggataaagtc atgattacag
    64621 acttaaaagt tataatatat gaagaaaaaa atataattag taagatgatg gaattattaa
    64681 aagaacaatt tgattatata gttgaaaaag acttagaaaa tttaaatatg ttaaatccta
    64741 aactagaaga tattagcagg gagcttgcat ccatagaaat aaagagaaga caactttttg
    64801 gtgatgatat ttcaatgagt gaagttgttg aaaatagtaa tgatagatat ttaaaagaaa
    64861 tatatacaga cttgaaacac attcttgctt tagctaaaaa tcaacaagaa tcaaatgatt
    64921 ctctaataaa gaaagaactt atttttacga aaaaaatgat taatttcata aaaccagctg
    64981 ataagcaaac tgcaacttat aattcttatg gaaatattag aaaataaata ttaggaggag
    65041 aaatatggca ggattatttt ccattttaaa tacagctaat tcaggaatga atgcgcacca
    65101 aaaagcaata cagacaattt ctcataatat aagtaatcta gatacagacg gttattctag
    65161 gcaaaggact gagtttgcta caaatagtcc aatgtatatg cctagtttga gtaattcaat
    65221 tgcaagagga caattaggga ctggagtaca cgtaacggat gtaacaagag ctagaaatag
    65281 tttttatgat taccaattta gagcagaagc tcataagtat ggtaatacag tttcaaagta
    65341 tgactattat aatactatag aaactataat aaatgaacca tcagactatg gaatatctgc
    65401 aggtatagac gactttttta aaggatggga atcattagca aaggatataa atagtgatag
    65461 taaaaaaagt ctagtggtag aaaatgcatc aaatctagca actttaataa gtgaaagtta
    65521 taagaagctt aataagttaa agggagatat agacgataat ataaactctg aaataaaaca
    65581 gataaatgac atgttaaaaa gtcttgaaga tttgaataaa tctattgatg ttatatctgg
    65641 ttctggctct actccaaatg atttacttga tgaaagagat agaatattag atgacttaag
    65701 ctttaaatta gaccttgaaa atagcgatgt taaaaatatg ctttcagatg gtaaattaga
    65761 acttaatgaa ttaaaaaatg cagatggaac ttggaaaaca ggtgtatcag gaaccttaca
    65821 aggcttgttt gaaatgcatg gtaagattga tacatataaa agcgatttaa aagatgtatc
    65881 tgatgggtta gctaagcaga taaatgatgt ttataataca tctgctggaa taacagtaac
    65941 agattttttt ataacttcaa atgtagatgg tgaagatatc ataaaggtaa atccagctat
    66001 aaagagtaac ccaaatgaat taaaacttac tgctgatgag gcttcaaaga ttgctaagtt
    66061 aaaggatgaa aaaatagata ttggggtggc tggtggaaaa gtaagtacaa taagtgacca
    66121 ctataaagcc tttgcagaat cagtaggttt agattctcaa aaagtgaatc aagatgaagt
    66181 gaatcaacgt aaaattataa ataatattga taactcacga atgagtgttt caggcgtatc
    66241 tttggatgaa gaaatgacag agcttataaa aatacaacgc tcctatcaag ctagtgcaaa
    66301 agttatgtca acagctgtgc aactgttgga tgttgtaata aatggaataa tttaatagaa
    66361 gggggaattt ataatgagag ttagtacagg tatgatgtct agcaactatc taaactcact
    66421 acaagataat ctacaaagat tagataaggt taatagacaa gttaatacaa cgaaagaaat
    66481 aaacaagcta tcagataatc catataaggc aataaaaata ttgaactcaa aaagtgaaat
    66541 aaaaactatg gagacttatg ttgagaattg taaagataca gggaattggt tagaaactac
    66601 tgatactgca ttagatcagc ttggaaaatt attatcggac ataaagaaaa ctttggtggc
    66661 aagtggtgat ggtgcatatt cagatgatga gctaaagact cttagtaatg aaactaatga
    66721 aaagatgaaa gaaattgcaa atgccttaaa tgcaacacat gaaggaaagt acatattttc
    66781 aggttcaaat acaggtgtac cacctgttga atgtatagat aatgctgacg gttcagtgag
    66841 cttaaagttt aattctagtt taaatcttaa taaattaaat gactctttga gtatggatat
    66901 agctcaagga ataagtgtag attataatgt taaattgagt agccttggat ttgacccaac
    66961 aaagactgac ccatttgaag agttaaataa tatatcaaag aaattagcaa atccagatgc
    67021 tgatgctata aaagagctga caacaacttg tttaggcgac atggaacaat taatagaaaa
    67081 tacagtaaat gttaggtcta tatatgggac aaaagcaaat acagtagagg ctatgaagga
    67141 aaaaaatgat gatagtttga tacagttaaa ggatattttg tcacaaaatg aagaaataga
    67201 ttatggagaa aaactcgttc aattaaaggc agcagagtta acttatcaag cttcactaca
    67261 aacaggagga aagttattta atgtatctat attagattac atatagtaaa ataacgcaga
    67321 tatgatgaag gttacattaa aaaaaggtat attaggtttt gaaaatttaa aagaatatga
    67381 gcttttagat atagaagata atgattcttt aaaagaattt aattctactg aagaagaagg
    67441 tataggattt gttgttgtat ctccatttga gattatcaat gaatatgaaa tagttttaaa
    67501 tcaggaaaca atagaaaaac tagaagtgaa atctccaaag gatgttgtac tatttaatat
    67561 aattactctt ggtcagacat ttgaagaatc aactgtgaac atgaaagctc ctatagtgat
    67621 aaatataaaa actaattttg gaatgcaaat aattttacag gatgataaat attcaataag
    67681 gcatccatta ttgagaggtg ataatggatg ctagtaattt caagaaaaaa agatgaagca
    67741 gtacttatag gagaaagcat agaagtaaaa attgtagggg tagatggaaa caatgtgaaa
    67801 ctagctatat cggcgcctaa caatataagt atattaagaa aagaaatata cgaaaaagta
    67861 aagagtgaga atataaaagc tacaaataaa aatataaaaa ttttaaagtc attaaaataa
    67921 taagcttgga ggaagtaata tatgtatgga gaaaatcctt ataattctta taaacaaaat
    67981 gctgttttta tggcatcaaa ggaacaattg ttacttatgc ttgttgatgg agcagtaaaa
    68041 tataccaaaa tagccagagc agctatctta gataaaaata cacagaaagc gcatagagaa
    68101 cttgtaagag ttcaggatat atttacagaa ctaatggtta cattagacca aaatgcagga
    68161 caatgggcaa aggatatgta caaagtttat gattttgtaa gatatgaact ttcaagagcc
    68221 aatattagaa aagacatcca ggtaatagat aatgtgttgc ctgtaataga acaaattaaa
    68281 gacacatggc atgaagctga taaaaaaagt aaagaagaaa gaagtaggta caaataatgg
    68341 aattaaattt agctgaatta gataagttat caaaagagga attactattg atgcttgtag
    68401 atggcacagt aaaatatacc aatatatcca aagaagctct tttaaataac gactatttga
    68461 aagcacataa tgaacttata agagttcaaa atatatttac agaactaatg actacattag
    68521 accaagatgc aggtcaatgg gcaaaggata tgtataaagt ttatgaattt ataaaatctg
    68581 aattagcaat agctgatgaa aataaagata ttaaaataat tgatgatata ttgccaatag
    68641 taaaacaaat tagagataca tggcatgaag tttacaatca actaaaaata taattagatg
    68701 gagggataaa ggtatgtcaa gtataagtcc tataagagtt acaggtcttt caggaaattt
    68761 tgatatggaa ggcataatcg aagctagtat gattagagac aaggaaaaag ttgataaagc
    68821 aaaacaagaa caacaaatcg ttaaatggaa gcaagaaata tatagaaatg ttatacaaga
    68881 atcaaaagat ctttatgata aatatctaag cgtaaattct cctaatagta tagtaagtga
    68941 aaaagcatac tcttctacaa gaataaccag ttctgatgaa agtattatag tagcaaaagg
    69001 ctcagctggt gcagaaaaaa taaattatca atttgcagtt tctcaaatgg ctgaaccagc
    69061 aaaatttact attaaattaa attcaagtga acctattgtt cgacagttcc ctccaaatgc
    69121 cagtggagct agttctttaa ctataggaga tgtaaatata ccaatatctg aacaagatac
    69181 tacaagtact attgtaagta agataaactc cctttgcgca gataatgata taaaggcttc
    69241 ttatagtgag atgacaggtg aattgattat ttcgagaaaa caaactggtt cgtcatcaga
    69301 cattaattta aaagtaattg gaaatgacaa tttagctcag caaattgcta atgataatgg
    69361 tatcacattt gcaaatgatg ctagtggaaa caaagtggca agtgtatatg gaaaaaatct
    69421 agaagctgat gtaactgatg aacatggaag agtaactcat ataagtaaag aacaaaattc
    69481 atttaatata gataatattg actataatgt aaattcaaaa ggaactgcaa agttgacttc
    69541 tgtcactgat actgaagaag ctgttaaaaa tatgcaagca tttgtggatg attataataa
    69601 actgatggac aaggtctatg gtttagttac tactaaaaaa ccaaaagatt atccgcctct
    69661 tacagatgcc caaaaagaag atatgacaac tgaagaaata gaaaaatggg aaaagaaagc
    69721 taaagaaggt atacttagaa atgatgatga gttaagaggt tttgttgaag atattcagtc
    69781 tgcatttttt ggagatggaa aaaatattat tgcattaaga aaactaggta tcaatgaaag
    69841 cgaaaattac aataaaaaag gtcaaatatc atttaatgca gatacttttt caaaggctct
    69901 tatagatgat agtgataagg tatacaaaac actagcaggt tattcttcga attatgatga
    69961 taagggaatg tttgaaaagc taaaagatat tgtatatgaa tattctggaa gttcaacttc
    70021 taaacttcct aaaaaagcag gtatagaaaa aactgcttct gctagtgaaa atgtatattc
    70081 aaaacaaatt gcagagcaag aaagaaatat aagcaggtta gttgaaaaaa tgaatgataa
    70141 agagaaaaga ctttatgcta aatattcagc cttagaatct ttgttgaatc agtattcttc
    70201 ccaaatgaat tatttctcac aagcacaggg taattaatta tggataaatt aaaagaaaaa
    70261 ttaaatttat ataaagatat atctttacaa attataaatt tgattgaaaa ggaagaatat
    70321 ataaagataa gtagtaagtt aggtgaaaga caagagatta taaatagtgt aagtgaaatt
    70381 gatagaaatg attttataca gctttataat agaatggaat taattgaaat tgatagtagg
    70441 ataagagata ttttacaagg acaattatta gaagtaaaaa aagaattaca tgagtataag
    70501 cttactaaac aagtgaacac aatgtattat aacttaaata gagaaaaagt aaatattttt
    70561 aataaaaaag tataaagtta tgtaccatct tgtccgatta tataaatgaa gaattaataa
    70621 aaaagattaa aagtagaaat gacaaggatg tcaactatac taaggagggt aaaataatga
    70681 gagttaatac aaatgtaagt gctttgatag caaataacca aatgggaaga aacgtaaatg
    70741 cacaaagtaa gtctatggag aagttatctt ctggtgttag aattaagaga gctgctgatg
    70801 atgctgctgg gcttgctata tctgagaaaa tgagagctca aataaaaggt ttagaccaag
    70861 caggaagaaa cgttcaagat ggtatatctg ttgtacaaac agcagaaggt gcattagaag
    70921 aaactggtaa tatactacaa agaatgagaa ctttatcagt acaatcttca aatgaaacta
    70981 atactgctga agaaagacaa aagatagctg atgagttgtt acaattaaag gatgaagttg
    71041 aaagaatatc aagctcaatt gagtttaatg gtaagaaatt acttgatgga agttctacag
    71101 aaataagatt acaagttgga gctaattttg gaacaaatgt tgcaggaaca actaacaaca
    71161 acaatgaaat aaaagttgct ctagtaaata cttcaagtat aatgagtaaa gcaggtataa
    71221 caagttctac aatagcaagc ttaaatgcag atggaacttc tggaacagat gcagctaaac
    71281 aaatggtgtc tagcttagat gtagctctta aagaattaaa tacatcaaga gcaaaattag
    71341 gggcacaaca aaatagacta gaatcaactc aaaacaactt aaataatact atagaaaatg
    71401 ttacagcagc tgaatcaaga ataagagata cagatgttgc ttcagaaatg gttaacttat
    71461 ctaaaatgaa tatattagtt caagcatcac agtcaatgct tgctcaagct aatcagcaac
    71521 cacaaggagt tttacaatta ttaggataaa ataattttgt atattggata tagaagaaag
    71581 ggtaaggctt tgctttactc ctttcttttt ttacaaatat acttttgaaa ggaaaatata
    71641 tatgttactt agtatagtta tgattgttaa aaatgaagaa aaaatacttg aaaaaacttt
    71701 aaaatcatta accacattaa gaagttctat agagagtgaa cttataatag ttgatacagg
    71761 ttctacagat gatactataa atatatctag gaaatacacg gaaaatgtgt actttcataa
    71821 ttggaataat gatttttcta gtatgagaaa tatatcaata tcctatgcta aaggtgaatg
    71881 gttacttata ttagatgcag atgaaatact aattgattct tcaactattg tgaaattttt
    71941 taaagatggt cttgataaaa aatttaattc ggcctcaata caattaaaaa atctttactc
    72001 atatgataaa aaattatatg gatattgctc agtgcttaga ctttttaaaa acatcgattt
    72061 tagataccat ggtaaagtac atgaacaacc aatatacaaa aaccctatat ttaataatgt
    72121 tgctgatttt gaacattatg gatatttgtt tgaagatgaa gaaataagat taaataaagt
    72181 aaaaagaaat gaaaaactgt tatttgaaga attaaaagag aatgataatt ctccatatac
    72241 aaattatcaa ttagggaaaa attttattat attaggaaaa tatcatgagg ctttatacta
    72301 tcttgagaaa tcttatgaat tatattctaa actagaatcg gcacctggtt acttgataac
    72361 aagtcttgta aaaacatatt tatatttagg aaagcataag aagtgtgaaa aattgtgtct
    72421 aaagtatatt aagaaagaca tgaataatat tgatatatat tattatattg cacaagcaca
    72481 agtagattta gggaagtatg aaaatagtat agattcgtat aagagatata tttatttgtt
    72541 agataattat gaaataagta cacaagcaaa tagtttattt tcagacactg atactgtagg
    72601 gcttagggat gaagctatta ttacactgat aaagatttat tataaacttg aaaaatatga
    72661 gtctgtaatt tcagagtaca ataacattga ggatatggaa aagaagaaga atgtgtactt
    72721 tagcttattt atgtccctat acaaattgaa cagatttgaa gatataaaaa actattataa
    72781 agaagtttca aattctaaaa ttgaaaaaaa tagtttttat aaaaatatag aagaagttat
    72841 aaataatatt aaagatgatg aaaaagagca tgtatataaa atcctttgtg acattgaagg
    72901 taattatggc attttaaatc agataaggat aaataaatgt gtatctatag ataagtgtaa
    72961 agaaatactc aatgaagaaa aagaaataat ttatgcatat attataaaag tagcttttga
    73021 agataagatt gatttactag atatttttta tgaaatggaa tatgtatgga tagagaaata
    73081 cctaaaatat cttttagcac tagaaagaag ttttaattta gtactatata agtacataat
    73141 tagtcgtccg aatacatcag atataaaaaa gataagagta tataaagcta tatctaaagt
    73201 agttttagaa agtataagtt taagtgatga aaaatataag gagatttttt atctttatat
    73261 aatgtattct tataagtaca taagatacat atatagaaat ttaagcgatt ttgaattaat
    73321 aaaatttgtt tgcaatgatt tagataagtt tactatagaa tttaagcatc tacttgacat
    73381 taaaaataaa gtaaatgatg ctaatgaaaa attagagtat atacatagta tgaggaaatt
    73441 actaaatgag tatccttttt ataataaaat aataaaaata cttataagtg aattagaaga
    73501 aaatataaaa gaaagtgaag aatttaagga tttaaaagat agttttatta ttagtataga
    73561 aaatatgatt gaaactggca aaataaatga tgcgaaatca ttggtagatg actattcaaa
    73621 aacatttatg gatgatgtaa aaattttaaa tataaaggga attttatata tgtttgaaaa
    73681 taagcttgat aaagctgatt ttatgttaaa aaaggcatta tcattagatg tagaaaatga
    73741 agatactata tataatatag aatacttaaa aagtttaaga taagtcaaaa atgggagaat
    73801 tgaagaatga atacaccaat agtggttaat gaaaaatcta aagatataat aaaagattgg
    73861 caatatacgt atacaaacga agatataaaa tttacaagca taataatatt gacatataat
    73921 caaatagaat atactaaatt atgtattgaa agtattagga agtttacccc aaagaataaa
    73981 tatgaaataa taataattga caataactct tctgatggaa cagttgagtg gttaaaggga
    74041 caatctgaca taaaatctgt atataataac aaaaacctag gatttccaaa aggatgcaat
    74101 caaggtattg agttggctac tggtgaaaac atattgttgt taaataatga tgtaatagtt
    74161 actccaaatt ggctttataa tttgaataaa gctttatggt ctcaagataa tataggtgct
    74221 gtaggtccta ttacaaatgc atgttcgtac tatcaacaaa ttgatgttga ttataaatca
    74281 attgaggata tgtttatgtt tgcacacaaa ataaatacat ctaatattaa cctatggaaa
    74341 tatagattaa agttagttgg attttgcatg ttaataaaaa aatctgtgct ggataatgta
    74401 ggggttttag atgaattatt cacacctgga aattttgaag atgatgattt atcttttagg
    74461 atgctagagt caggatataa gctattatta tgccaggata cttttataca ccattttgga
    74521 agtgtatctt ttaaagaaaa tcgtaataaa tatacagata taatgtatat taatagaaga
    74581 aaatttgaaa ctaagtgggg atttaatcca gaatatagtg gaatgataag aacagatttg
    74641 atttctaaaa tagatgatag tttggaggca aatattaatg ttttagaaat aggctgtggt
    74701 ataggggcta ctcttttaga aataaaaaat atatataaaa atgcatcaat atatggaata
    74761 gaaattagtg agtcagcagg taaacttgca aagtatagtg cagatgtact tatagcagat
    74821 atagaaaaag taaagttgga ttatgaaaaa gatttttttg attatataat attgggagat
    74881 gtacttgaac atttaactga cccatggaag atagtcaatg aactgaaaca atatctaaag
    74941 aaggatggtt gtttgatatc tagtatacca aatattatgc atgtttctgt tttaagaaac
    75001 cttgtgttag gtaattttac atatgaagat tcaggaattt tagacaagac tcatttaagg
    75061 ttttttacat taaatgagat aacaaaaatg tttaatgata gtaattacat aataaatagt
    75121 gttacagcta ctagtatgcc actttcggaa attgataata gattcattga tacaatgtgt
    75181 aaggaaagct gtaaagaaat gagacaacag tacgaatctt atcaatatat agtaaaagcc
    75241 actaaaagca tagatactaa aagatattct agtattagta tggttaattt aaaatacgct
    75301 ttaatgagaa ttgataatga attagatgta gatagtaatt taagttatat atttgataat
    75361 tataatataa ctgaaccatg ttttgttgat gatattgaat atttagttga tacgtttgtg
    75421 ataaataaag caactctatt aaatagatta ggagttgagg catttaacag aggtttagga
    75481 gattttagta taaacatgtt tatgacagta ttagagatgg atagagatga tattgattca
    75541 atatataata taacaagtgt attatgtgaa ttagaagaat ttgatactgc ttacaactta
    75601 attaaaaata gtagcaaaaa agtacagagt gaaagtgaca tacaagatat actttatttt
    75661 attgagggga agagatatga ataatgataa gatatgtttt ataacctgtg taaatgatga
    75721 aagactttat gaggaatgtc ttgtatatat aaatagccta gacattccta aaaactttag
    75781 catagaaaca gtagctttaa gaggagccga ttctattgcc tctgcttata ataaagctat
    75841 gagagaaaca gattctaagt ataaggtata cttacatcaa gatgctttta taattaataa
    75901 aaatttcata tatgacattt tagagatatt taaaaaaaat acagatgtag gaatgattgg
    75961 tgtagctgga gtaaaagata tacctgtgaa tggaatttgg tgggattctg ataaaaaagc
    76021 agggaaagta tatgatagcc atacaggtaa aatggatatt tatgaattta gtaactttga
    76081 agaatgtttt aatgaagtta tggctataga tggattaatt atgattactc aatatgatgt
    76141 attttggaga gaggataagt ttgatgggtg gcatttttat gatttgtcac agagtatgga
    76201 atttagacaa aataaataca aagttattgt accaaaacaa aataagcctt ggtgtatgca
    76261 tgattctgga gtagcaaata taaataatgg atatgatgaa tataggataa aatttgtaaa
    76321 agagtacata aatcttgatt tatatagaga aaatttttat gaatttgata agaagaaagc
    76381 aagtaccata cttaagagta ataagcatgt aatggattgt atgaaagagg agcttacaga
    76441 acaattaaga ttaaagaatt ataaggatgt tttagatatt gtttctaaaa ttgctcattt
    76501 ttctgttttt aatcatactg gcttgtattc ttgtgaaaaa tgtgagagag cattgctaga
    76561 atgtgcaaat aatttaccag aaatagaaaa tacgaaatta tttacaaaaa aagatttaaa
    76621 aagaagaaaa gtattacatg ttttaagtga aggatattca acaggagggc atacaagact
    76681 agttaaaaat tggattgaga ttgacaaaga ttctgttcat tcagttgtaa ctacatggca
    76741 atcagataca ctgccagact ggttattaaa agcaatagat gatagtggtg gcgaagttat
    76801 atctttggat ttaatttcag atgattattt aaaaagagct gtagctttga ggaaaatagc
    76861 ttatgactgg gctgatatag ttattttata tgttcatatg tgggatacaa tacctgttgt
    76921 tgcttttgga gttgatggga aaatacctat tttacatgtt aatcactatg atagtacttt
    76981 ttggttaggg acaagtattg cagatattgt cattgatttt agcgaagcta gtcaattaat
    77041 tacattagat aaaagaggca tagataaatc tgaaatatta ccaatgccat taaaagaaaa
    77101 agaagtattt tcaaaatctg aagttagaaa acgatataat ttggaggatg atgtgacaat
    77161 aatattgact attgctagtg aaagaaaata caatagtgta aatgatatta attactttaa
    77221 aattgctaaa acattgattg aaaatacaga taatacaatt attattgttg taggagttga
    77281 tacagagaat ctctattgga aaactttaag taacgttaca gataatagag ttatatgttt
    77341 tggaacaaca gcaaatatag acgattttta taagatttcg gatatatacc tagagtcttt
    77401 catggtaggg agtcatactt caaaattaga tgcaatttta tatgatttaa ttcctgttaa
    77461 gtttaaaaat gaagtttcac caatttttac aaatatgtgg aattttatag ataagtttga
    77521 ttataagaat ataaaagagg ttatagacat gataagaagg tacaatacta gtgaatttga
    77581 cagggataag ataaaagaaa aacaatcaga aataaaacat tttgttatag ataaacactg
    77641 tattgaaata aaaaactata tagacaaaat atataaagag gctaatcatc ataaagttaa
    77701 gtatgattta tataaaaatg atgaaatcga agactatcag ctttttgttg cattatatgg
    77761 ttatatggta aaaaataaaa acatgtcata ggtgataaaa tgaacaatat tcatatcaat
    77821 aaatttgaaa aaagctgtga aaaaaactgg gattattttg tagaaaataa atctataaat
    77881 ggtacatttc tgcaaactag aaatttcctt aattatcatg aagaaggaaa attcactgac
    77941 cactcattaa taatatataa aggtgaagaa atactagcag tcgtacctgc atgtgaaact
    78001 tatgaaaatg gtgaaagagt atttttttct catttaggaa gcacttttgg aggtattata
    78061 ataagaagtg gttttaatga tattaggcat gtaaatgcta ttacaaataa gttagaaaca
    78121 tatttacagg aacaaagttt caataaagta tatttaaaaa atacatcaag aatattttgt
    78181 aaagaggata cttcattact agaatatttt ctatttaaga ataattacat gttttatgat
    78241 gaattaagta tgtttttaga attaagtaag tataaaaaag atatattagc taattttaga
    78301 tatagtaaaa gaagatattt taaatattct ttgaagaata atcttacctt taaaagattg
    78361 tatagtgatg aggatatagt ttgttttttt aatttacttt ctaattgttt agatagtaaa
    78421 ggagctaaag taatacattc agtagaagaa cttttggagt ttaaaaataa tagattaaaa
    78481 gatattgtag aattttatgc agtatatatt gatgacatta tgatttgtgg gagcatggta
    78541 tttaactttc aagaaagagt ttatcatact caatatctag catcaaattc gacatataat
    78601 aaatattatc caatgatatt tttatactat aatctaataa aagatgcttt agagagaaat
    78661 tttaagtatc tttcttttgg aattagcact ctaaatagag gcaaatactt agacgagggt
    78721 ttagctacat ttaaagaagg ttttggttca agtgcaattt gcaacagaac atactataaa
    78781 aatataaata agtgataatc atgttttata gagtttttag agtatagtaa atatctagta
    78841 ctctaatatt acataaaaat aattagttta taaagaaggt ttgtatatga aaagattaat
    78901 gattttaggc tcaattaagt attttgagaa tattgttatg tcagctaaaa aagagggaat
    78961 atacacaatt gtttgtgata atagaattga tactcctgcg aaaaagattt gtgatgaagc
    79021 aatagatgtt gatgtatttg attttgataa aataaaaagt atagctatag ataaaaagat
    79081 agatggaatt ttaactggat ttacagattc tttaatgaaa ccatatgttt atattgcaaa
    79141 tgaactaaat ttaccatgtg tgatttcttg caatcaactt gaatctgtaa ctaataaggc
    79201 tattatgaaa aaatatttta aaaataatgc aatacctaca actgattatt gtattattga
    79261 aagtctaaaa gatttagaaa aaatacaagg attaaatttt ccacttgtaa taaaacctgt
    79321 tgatggttat ggttcaagag gtgtatattt tgtatcaaat atagaagaat taatagaaaa
    79381 atttgaatat tctaaggaag cctcacaaga tggacaagta ataattgagg aattttatga
    79441 aagtgaggaa atacaaggac tagcttgggt tcataatgga gagtcacatg tattttatat
    79501 tggagataga gagcttgtaa atatacatac aggaagagct ggaaaaccag atagattact
    79561 ttatccatca aagtattgtt ttcaatatga ggaagaaata aaatctatat atcaaaatat
    79621 aacacaggct tttgatataa aaaatggacc tctttatgta caaatgttag tgggagcata
    79681 tggaataaag gtatctgaag taatgccaag attacctgga ggttgtgact atatggctat
    79741 ttctcaaata acagatttag acataggaag tttatttgca aactttagtg tgaataatga
    79801 agtaaatttt gatgaaataa agaaatataa tatgcattta tcaaaatgtg tatatgcatt
    79861 accaatatac ataagacctg gaacaataaa aaagataaat aatatagata aaatagaaaa
    79921 cttagattat gtcactcaat ttctattatt agtaaaagaa ggagatacaa taggattata
    79981 tgaggatgag agacaggatt gtggaagagt ttatggaata gcaaataata tatttgatgc
    80041 aaatataaag aagaaatgta ttcatgaaat gatagaaata tttgatgaaa ataatgaaaa
    80101 tatgatagaa aatttttaaa taagggagta tgatagatat gaagctaata actcatgaac
    80161 aaattatgtc attaaacatt tctcagaaac aaggatatga atggataaaa gaatcttttt
    80221 taacaaaaaa agattgtcaa ttaccaccaa aaataagcat ggtagaagaa ggtcatgtat
    80281 tttacaatgt aatgccgtcc ttaaatcata accatatgat tggtggaata aaaatggtta
    80341 caagattgcc agggagaaat ccaagtttaa attcagatat atttctatac aatatgaaag
    80401 atgctaaatt aaaggctatt ttggatggta attatattac agcttttaga actgctgctg
    80461 ttgctggttt ggctattgat acttttgcta aaaaagattt taatactatt tctattatgg
    80521 gtttaggtgt aactgcttct gctataatga atatgctttc gtatactctt aaagataaaa
    80581 atataaatat aaagttactt aaatataaag accaagcaga gtcatttata aaaaagtatg
    80641 aaaaaaatga acaatttagt tttgaaatat gtgatacaca tgaagaactt attgtagatt
    80701 cagatgtagt tatttctact gtgacatata cagatgaata tttatcagaa tttaaatggt
    80761 ttaaaaaagg tgctttatta ttacctgtac atgttatggg atttggaaat gttgattatc
    80821 tatttgataa agtatattta gatgattata atcatttgaa acatctagaa aatatagata
    80881 gatataaata ttcttgtgaa ttttataatg ttttaagtaa acaagctaaa ggaagagaaa
    80941 atgatgatga gagaataata tcttacaata taggtttgtc aatacatgat atattatttg
    81001 caaacaaaat ttacgaattg tgtgaaacac taaattgtga agagatagaa ttaaaagaac
    81061 caaaagaaaa atattggata tagtttattt cattcatatt tacaataatt tattgtaaat
    81121 atgaatatga agtaatatat tgttagaaaa ttagcatgaa taggatagat aaatttatgg
    81181 ataaatataa tttaattaag ttcttatcta ttggaaacga atatatagga aatttagttg
    81241 cattagaatc aaataaagca ataccttttg atataaagag agtttattac atatatgatg
    81301 taccaaacaa tgtaaagaga ggatttcatg cacacaaaaa cttggaacaa gtactagttt
    81361 gcctaaatgg aagtgtgaag ataaaatgtt atgatggtgt aaaagagagt atatttaatt
    81421 tacataaaaa tgatgttgga ttgtatatag gaacgggagt gtggcatgag atgtttgact
    81481 ttgatgaaaa aactgtttta atttctatag catctgaaca ttacaatgaa gaagattata
    81541 ttagagacta taaagaattt ttaaagtatt taaataatat gtagttatta tacagttagt
    81601 gatgtatttt cttaattaaa taaatttaag atagccatat agagaataaa tagattagga
    81661 tgtgaattta ttgttggtta aatttaataa tttattaaaa cagttccaaa ctcataaaga
    81721 agaatatgaa cagaagatta aggatgtaat ggaaagtgga tattatatcc ttggaaatga
    81781 agtaaggcat tttgaagaag aatttgctag ctatgtaggt agcaaatatt gtgttggttt
    81841 agccagtggt ttagatgcac ttataattgc ttttagagca ttaggaataa gtgaaggtga
    81901 tgaagtaatc gtgcaatcaa atacatatat tgctagtgtt atgggaatca gcataaatgg
    81961 agcaacacca gtatttgtag aaccaaatga atattataat atagattcat ctaagataga
    82021 agaaaagata aatagcaaaa caaaagctat attagtagtt catttatatg gtcaagcaag
    82081 taaaatgaaa gatatacaag aattatgtga taagtataac ttgtatttaa ttgaagattg
    82141 tgctcaatct catggagcta aatatgatgg aaaaatgaca ggaacatttg gtgatatagg
    82201 ttgttttagt ttttatccaa gtaaaaattt gggtgcattt ggagatgcag gagctatagt
    82261 aactagtaat gaggaaatag ctgataaagt gaaaattctt agaaactatg gttcagaaaa
    82321 gcgatattat aataaatttg tcggatataa ttcaagattg gacgaaattc aggcaggttt
    82381 attaagagta aaacttaaat atctaaatga aattgaaatt gaaaaatcaa aaatcgtaga
    82441 tagatatctt aatgaaatta ataatcccaa agtcatactt ccaaagatag aaaataattg
    82501 tactcatgta tggcacttat ttgttttaca agttgaaaaa cgagatacat ttatggaata
    82561 cttaaagaaa aatggtatag agtctataat acattatccg ataccaccac atctatctga
    82621 agcatatgct tatttgggat ataaaaaggg gagttttaat atagctgaga actattcaga
    82681 taaagttgtt agtatacctt tatttgtcgg tatggaagat gaagaagtta gttatataat
    82741 taaaattata aatgaatatt aaactaatca taataatatc aaaaataaat tttctatata
    82801 tcatttattt aaatttcgtt aattgacatt tttaaatatt gatatattgt acaaataaaa
    82861 ttgaaatata tggaagaata tagaataaag atgagatttg tctaatattt ttataaatta
    82921 aaaatatttt caaagagttt attataaaat tgtagactat agtgtttata atctttgtaa
    82981 ttattggtaa ctaaatttaa aaaataaaat atttaattat acacaaaaaa gatatttaaa
    83041 aaatttgtta tttattgtat acttaagtta aaactaaata ggcaaatcta gagaaatcta
    83101 gtgacgcaaa gctatagggg ctaaggtttg tatacacaaa ctatgtcagc cagttgccaa
    83161 aaagagttct aggtatattg tatacgtagg aagagtctag gatgactgcc tttttggtgg
    83221 tcattttgtt atggaaaaaa tcacattatg tagtaaaaaa cacctataat aacatattat
    83281 accaaatata atttaaaata aagataaatt atctataaaa gttactat
//
LOCUS       DAMJVX010000002        73101 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMJVX010000002 DAMJVX010000000
VERSION     DAMJVX010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 73101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73101
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.002"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(547..2223)
                     /locus_tag="PC407_000076"
     CDS             complement(547..2223)
                     /locus_tag="PC407_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2495563.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIDGYSLEAQKSRMKAFALYNDYEIV
                     GEYEDAGKSGKSIEGRVQFTRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDFGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRVQTMEGRIQKAKEGKWNG
                     GFAPYGYQLIDGKLIINEEEAIAIRTIFDQYVNTSIGANGLSKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYRQVEQDDYLVAEGIHEAI
                     IPDELWQAAQVKLKAQAKKYEHVNKGKNMRTHLLSGIVKCPICGAGMFGNKSIKYKKD
                     GTKYKDFYYYGCKHRLMNRGHKCTYNKQIREELLDDAVAEVIIKLVSNPKFASMIQEK
                     INMKVDTSAIENEIDNYQNELRKSHSTKYKLIEEIDNLDVEDKHYKRRKNDLDDRLYR
                     MYDKIEELESQLIEAKAKKETIEVEKLTGDNIYKVLIYFDKLYKVMNDVERRQLIEAL
                     ISEIQIYEEKQPNGQWLKSITFKLPIIDEDLNISLENDEQVETVAKIIKQ"
     gene            complement(2324..2476)
                     /locus_tag="PC407_000077"
     CDS             complement(2324..2476)
                     /locus_tag="PC407_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495564.1"
                     /translation="MDNDWNGLADLIANLITKYAGVLDLENLPNPTPVKNTEDKNKFD
                     IAKSTD"
     gene            complement(2965..3375)
                     /locus_tag="PC407_000078"
     CDS             complement(2965..3375)
                     /locus_tag="PC407_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2495565.1"
                     /translation="MAKEYYLYIRGQKVKVSEDIYIVYWREKEHEKYLEQVDRKNHLL
                     FFSALDHDGHFTDNIVDESVDVEKIVETQMMIEAVRSAITRLNAEERDIIERLYFNDE
                     TVRSVAKLKSITHPALIKRRNKILEKLKKFIEEL"
     gene            complement(3899..5218)
                     /locus_tag="PC407_000079"
     CDS             complement(3899..5218)
                     /locus_tag="PC407_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2495566.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLVFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLELYSKSSEIKAFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKTRLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKALQDEEFFIFKIKIFS"
     gene            complement(5215..5868)
                     /locus_tag="PC407_000080"
     CDS             complement(5215..5868)
                     /locus_tag="PC407_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2495567.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYNDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            complement(5966..7342)
                     /locus_tag="PC407_000081"
     CDS             complement(5966..7342)
                     /locus_tag="PC407_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2495568.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKIFQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSSKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDVHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFVGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIARQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFIIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(7342..7998)
                     /locus_tag="PC407_000082"
     CDS             complement(7342..7998)
                     /locus_tag="PC407_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2495569.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQKKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(8008..9285)
                     /locus_tag="PC407_000083"
     CDS             complement(8008..9285)
                     /locus_tag="PC407_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2495570.1"
                     /translation="MIKNAIAYITRKRNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIGENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNKSENNKIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKHIIKIMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMLNITTLGQ
                     SYLILISIIVLSVVFASSLILIKKPKEILSKIS"
     gene            complement(9304..10194)
                     /locus_tag="PC407_000084"
     CDS             complement(9304..10194)
                     /locus_tag="PC407_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2495571.1"
                     /translation="MDRIKNIINGSTKKDKYKIRDRFRHLFQSVWFLITNSYFEGFKT
                     GKIYGGNLKKICVPGMNCYSCPGAKGSCPIGSLQAVIGNSKYKISYYIVGILFFIGAL
                     VGRFVCGWLCPFGLIQDLLHKIPFFKKVETFKFDKHLRKLKYIILLVFVIILPLFLVD
                     ILGQGSPYFCKLICPIGMLEGGLPLVLLNKSMRNAIGFLYYWKGIILIVTLLLSIIIY
                     RPFCKYICPLGAIYSIFNPISIFRYRLDKDKCINCGKCKKVCQMNIDPVENCNHLECI
                     RCGRCKNACPVDAISYGVRK"
     gene            complement(10187..10282)
                     /locus_tag="PC407_000085"
     CDS             complement(10187..10282)
                     /locus_tag="PC407_000085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2495572.1"
                     /translation="MAMIYYGHSNGETKVVLDKAIRICMECIGIG"
     gene            complement(10361..11518)
                     /locus_tag="PC407_000086"
     CDS             complement(10361..11518)
                     /locus_tag="PC407_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDF2495573.1"
                     /translation="MKKIVSRGACLLLTLCLSLSTVGCKKSNESNKIKEGQSISSEEA
                     GMSTNEKDKNETFKPSDYTLKTKKEYVYEYLGLKFKLSDKFKKYMDDKKIAMLDDQSP
                     IDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELERIGTIGIFEKNTSEEKISK
                     ITKCDTHTKIGVSSDGKYDCYLSTNSGAESNLLDELKRTEIQIIDKKERPKNGFVLSE
                     KTDLENTEAFNKESVKDLRKLSTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEI
                     PDLVEVQNEMKSKGVNIVGVVTDTVDDNGENKEAIEKSKLIHEKTKASYPFLMPDKTN
                     FNGRLNGIQAMPETFFVDSNGNIVGDTYSGAKSSKEWKQVIEKELEKIKNK"
     gene            11852..12202
                     /gene="mobC"
                     /locus_tag="PC407_000087"
     CDS             11852..12202
                     /gene="mobC"
                     /locus_tag="PC407_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDF2495574.1"
                     /translation="MANRIRNERLEIKLTEEEKALFEEKRKLARCRNMSHFIRKCVLE
                     KEIYQVDLEPFRDLQGLLSNATSNINQIAKRVNSTGIIYKDDINDMKKQIEYFSKELW
                     QIHSLLLNRTSGGD"
     gene            12208..13539
                     /locus_tag="PC407_000088"
     CDS             12208..13539
                     /locus_tag="PC407_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDF2495575.1"
                     /translation="MAITKIHPIKSTLNLAISYIVNGKKTDEQILVSTHKCHQETAHT
                     QFLRTRNDVGTNGTVLARHLIQSFLPGEASPKMAHQIGLELCKKILKDEYEFVLSTHI
                     DKGHIHNHIIFNNVNMVTGKCYQSNKKSYHKIRYQSDKLCKENNLSVIDEFYESYKRK
                     YKTNGKSWYENEQSKRGTSWKSRLQFDIDRLIKQSKYWEEFLKKMAELGYEIKYGKHI
                     AFKPKDKTRFTRAKTIGEDYTEERLKERITENQSITTPPVKRRIGNIINMNANAKVKE
                     SKGYEYWATKHNLNTMAESVIFIREHGINSIKQLDKYIQKSAEERQDLQDKMKEIDKD
                     MQLLSDTMEQVHTVKKYRAYYKEYKANPSDKAFFEEHKAEITHYEIALSKLKKSYSKL
                     PNSKDILDKLNKLQEKKNTLIQEYSSTKSTMDELYQIRKNYGIYMGKEMER"
     gene            complement(13575..14381)
                     /locus_tag="PC407_000089"
     CDS             complement(13575..14381)
                     /locus_tag="PC407_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HDF2495576.1"
                     /translation="MEISKKELRKISRKFRILASNVMNSYYGEQNANLEEMINYIEQT
                     LLIKEYLHSLEYDVDGLDDLLNTISNSYGRKVLNLGNDSNKRTYLLYRAFSFITGNNL
                     PTYTFGWYYAGSNKYQDMAKAFGDRMVYPFVLEVENYMKDISTDMGYDENNALYNINV
                     NSSGVQVNIAEHGSTINADQENKFNTEEVIKAINNVEDLIKGMEDDNSKSILNQNLDN
                     IKNEIKQKHPKKEVLTTCLKSMHFITTSVALIPDLALGIKMLASLIGISI"
     gene            complement(14371..14832)
                     /locus_tag="PC407_000090"
     CDS             complement(14371..14832)
                     /locus_tag="PC407_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDF2495577.1"
                     /translation="MANDIKFEISVPADEEGYVQLKCPSCSEKFMLTVEDIEAESTID
                     IWCPNCGLTHENYLDDDIYELAERIVENQVAEMLNDFSKNLEKSFRNSKSIKFKRGKE
                     IKKEAELPIGRKTGDYEEKKYLCCGKTARISSIKKLEGSYCPFCGEMIDGD"
     gene            complement(14860..15927)
                     /locus_tag="PC407_000091"
     CDS             complement(14860..15927)
                     /locus_tag="PC407_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDF2495578.1"
                     /translation="MSGKLYIAAAGSGKTRKIIEDSLNTHKKILILTYTITNEQQIIS
                     RIKNKVGVIPNNITVSTWFSFLLKDGIRPYQGSRFKERIEGIIFVNGQNNTYVKKTSN
                     KYFAKDNRVYTDKLSECVVELNDKCNGKVFERISGMYDCIYVDEVQDMVGYDLEILKC
                     LIASRIEVIMVGDPRQCTYSTHSSKKYKKYSCGKIDEFLKNECRKLDVRIDETTLNCT
                     YRNNDIICKFANSLYPERTSTNFVRRAEDESDGVMIIDKADVEKYLQKFNAMQLRENK
                     RTSVNNEYPYYNFGESKGLEFQRVLIYPTQPMWDWIINNNTSLKEQSKARLYVAVTRA
                     ENSVVIVRKDARPCSLPVVKL"
     gene            complement(15924..17546)
                     /locus_tag="PC407_000092"
     CDS             complement(15924..17546)
                     /locus_tag="PC407_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2495579.1"
                     /translation="MSIRKLKIKNFKCFSDWFIVDFENGINILVGNNGTGKSTILEAI
                     NLVLTGTYHGKNIRNELTQYLFNKNIVENYIREVNEGVNVEPPKIIIEAYFDAEMPSF
                     LGDGNSEKTKDTPGICLSISFDEKYSNEYKMLCKNKITSLPIEYYEVSWMSFARENIT
                     ARSIPIKSAMIDTTSYTPTSGSDIYISRIVKNLLDDNEIINISQAHRLMKEQFSRDEN
                     IANINRKLTETSNNELTISVDFGTKTSWESSLVTQLNEIPYNNIGKGTQCIVKTQLAL
                     SDHKIENKQIVLLEEPECHLSYSNLNILLDKVNESVKEKQIIVTTHSSFVSNKLGLEN
                     LILLNRGKTLRLGDLSNETNLFFKRIAGYDTLRLLLSNKAILVEGDSDELIVQRAYMD
                     SHNGKLPINDGIDIISVGISFLRFLEIAKLLELRVSVITDNDGNIDALNRKYSDYLGD
                     NCCENINIYFDDSTHEYNGSLENYNYNTLEPCIYRSNSMSILNEILNKSYEDPDDLLK
                     YMKGHKTEVALKIFESDKAIEFPQYIKEGILI"
     gene            complement(17749..17979)
                     /locus_tag="PC407_000093"
     CDS             complement(17749..17979)
                     /locus_tag="PC407_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2495580.1"
                     /translation="MRVFSYKRLFKKLIDLDMTNNELMEKAKVSKSTFYKMKNGQNVT
                     TDVLLRICNALDCDIEDIMECVIIEDVRRMGV"
     gene            complement(18119..18778)
                     /locus_tag="PC407_000094"
     CDS             complement(18119..18778)
                     /locus_tag="PC407_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2495581.1"
                     /translation="MDYKTMRDRVEDMLSDNHKDFVKAIISMEKGINDESALDKLYDA
                     YMDNDSLNLLHEEFDYMIEDLRGQGQIKDLLYVREEKDNLINIVGNIVGEVDVIEREN
                     KNGETFKVANFSVVSKDDEGNKLYHNCSVYGEKSDIPKDFKQGDFVKLFGQIRTSIDD
                     NGKEHSNIRILSSKLLKAKEQMKGQEEKKESILGAIKKYQAEDKEKPKEKKEASKEAE
                     R"
     gene            complement(18821..27550)
                     /locus_tag="PC407_000095"
     CDS             complement(18821..27550)
                     /locus_tag="PC407_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2495582.1"
                     /translation="MRINDFHNILELIKQDVLYNEAEYLKLLKVVGNNQRYDFRSQLS
                     IYDKNPEATACAKFDYWRERFNRTVMRGQKGIPILEDYGTYKKVDYIFDISQTVSKNR
                     DVNEVNLWRFDKEAHQDVLKEMIKSEGYEESESTLENIFSLSRLYGDEKIDTLMNELR
                     VADEDRISFTKFVRDSVSYAVASRFKLDYPMDYELLRENFQRFDSISLMSLGESVSDI
                     SGKIIDETIQKSKELELQKEVLIGKEAGYNKIKEELEEVEENVFRRDDQERNENERVL
                     RNGEYGRDNRKNQGEYAKQLGGTDGLYKEVSESDLRSDEAELPFTERGAEPLRDAFGS
                     IQGEEADRTPDGYSETGSRVYESREAKVDSSLEDRGREQSAVWGDDFSSQRNDHQGSS
                     GDLKDNTDKGIIEAEKASFSLPENSYGQISLTIPLTEKDIDTVLINGGNHDGGRLPVI
                     AEFSKGKSIEELGEYLKDTFKGGNGFYIDEREVSSWYSDKGIHLAYGTSAREDNTQIL
                     SWSDAASRINELLDRGEFATNVELLEAQDYERDRISESLWYLTHDLSEEGIEQGYFNS
                     FERGGGFPQETKRLSEALKNPEYLKETIKEYSRFLEGYKESRNVLRYHYHKVDSLYQK
                     LQELELQRKEYSTNLTELPKVKLFITEDEVLESLSRGSGVDRGKERITKFFKENHTLQ
                     EKANFLKDEYGIGGHSHVVSGAMGSDEWHDAKGFKLQKNDCNDVFLTWTSVAKHIDEL
                     LSKKLYFEEKETESTAEIEEVKEPQYYSKNDPGNLMPDEILERVPEIYAQEDVDLADK
                     QVHAAYIIPFRSNWTWYMTEYDRESGDAFGLVLGIEPEWGYFNLEELKELNAQRLILE
                     DFPKTFRELKDIELIKQMDEQELQFVFNGELSFEDKVELEAPEETEEATKRGTVQATL
                     FDYLKEREEVELNEKEDSLLEEFAVKAGDTVYFNHEEYKVREISKNEITGRYDLWLDP
                     VRSGNHQIPIVDFTDNEDLLNKISLERPNFIVGDEVKYKDKNHTITRFDDMRKNLNTV
                     TVKDNTEYFGGMITSFDVIPYRLESDLERVFENLTYKQPEQTTEETQIRKAEAHNFKI
                     TEETLPEKLSPSERLNNNLEAISMLNRVESGQRELDSTAQEVLARYVGWGGLSEVFDE
                     SKEGQWKEARAFLNENLSHAEYEAARESTLTSFYTPKTVIDGIYKTLSDMGFKQGNIL
                     EPSMGIGNFIGNIPDEMNKSKFYGIELDSVSGRIGKLLYPESEVQIKGLEETSFSNNF
                     FDAVIGNIPFGEYKVNDREYNKNNFLIHDYFFAKSIDKVRNGGIIAFITSSGTMDKKD
                     ESVRRYLAARAEFLGAIRLPNDTFKGIAGTEVTSDIIFLKKRDSIRERDEDWIHLAED
                     ENGLTYNKYFVDHPEQVLGTMREVSGRFGNTLACLPKENADLKELLTKASEEISKGST
                     YEEIELLDDEITSIPATDDVKNFSYTVIDDEVYYRENSLFIKKEITDKNKEKIKDYLE
                     LNTALKDVIYKQKEDYSDDEVKKAQEKLNEVYDNFSKKHGFINNLSNTRALKEDSNFP
                     LVSSIEILDEEENVKAKGDIFSKRTITKAKVIDHVDTSLEALVLSVSEKGYVDFEYME
                     SLTGKDRPTLIEELRGEIYLNIREEQNFYRPLSFNLEDGDLPFACANGSNSYKYGYVT
                     KDEYLSGNIRDKIAIVDSYLAKLRQTERELPHLGFVENGKEKELISYEMNRLEYQKAE
                     LTKVLPKELEASEINVRLGATWIPIKDIEKFIFETLKTPGYARWDIKVKFSNITSEWN
                     VEGKSRDKGNDLAEMTFGTSRVNAYKLIEDALNLKETKVFDQIINPDGSKTSVLNKKE
                     TMLAGQKQELLKEEFKNWIFNDQERRNRLVKLYNERFNSIRNREYDGSNLSFEGMNTE
                     IDLRPHQRNAIARSLYGGNTLLAHVVGSGKTFEMVASAMESKRLGMCSKSLFVVPNHL
                     TGQIGREFMQLYPSANIMVADKKDFEPKNRKRFIGKIATGEYDAVVIGHTQFEKIPMS
                     KEYQEKHIQDQINEIVNYVEEYKHDRNQNFTVKQLEKTKKKLETRLEKLNDDFKKDDV
                     ITFEELGVDMLFVDEAHNYKNLYLYTKMRNVAGIGQSEAFKSSDMFMKCRYMDEMTGG
                     KGIVFATGTPVSNSMTELYTMQRYLQYESLKKNHLEHFDSWASTFGETQSSFELSPEG
                     TGYRVKTRFSKFYNLPELMSMFKEVADIQTADMLNLPTPEANYEVIKTEPSEEQKEIL
                     KSLSERADDVRNRVVEPDVDNMLKITNDGKKLALDQRLINPLLPDNPDSKVNVCVKNV
                     FAIWDKTKENRSTQLLFSDMSTPKGDGEFNIYDDIREKLVAMGIPKEEIAFIHEANSD
                     KQKDELFAKVRKGEVRILMGSTQKMGAGTNVQNKLIALHDLDVPWRPADLEQRAGRIV
                     RQGNENEKVNIYRYVTENTFDAYLWQTIENKQKFISQIMTSKTPVRVAEDVDESSLNY
                     AEIKALATGDPKIKEKMDLDNEVTKLKMLEANYKSNRYRLEDKVAKNYPEEIARTEKL
                     IEAVKKDISNVEPKAEGEEKFTSITIAGEKITDKKLAGERLLEAISKVKINESKVIGK
                     YRNMDLEVSYNFFTNSHNFSLNGVAKHLGELGTSADGNITRLDNALEKMPEKLNMLEE
                     KLISTKEQLENAKEELKKPFEKADELKTKVLRLAELNKLLDMGEVEEKKNDNPLVEDV
                     KRAIIDFCNREYEENHSYDEFHTLYPDLKHIGIAYTNTPDERHGIQYELNLEEKTWTQ
                     YIDDTPIKTESFDYENKGENEALRNMKNEIELSTFSDLVYVDSEDLRVATGLDIDDEG
                     NFYDPFAKDLDNDGIPDRYDSDFKDSDYFESTYDVEDNLHSKEETTQKSDDKPSILGQ
                     IRAYQEESKTEEKQKIKEQEFLR"
     gene            complement(27633..28436)
                     /locus_tag="PC407_000096"
     CDS             complement(27633..28436)
                     /locus_tag="PC407_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004804477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDF2495583.1"
                     /translation="MSKINVEGSEISIIAIDNRDYISLTDMVRNIENGSALIEKWLRN
                     KNTIEFLGIWEEMYNTNFNSPEFEGIKNEAGFNRFILSVKQWVEKTNSIGIVARAGRY
                     GGTYAHKDIAFEFASWVSPYFKLYLIKEFERLKEEEQKKLGWDIKRNLAKINYRIHTD
                     AIKNKLVPDKLSKEKINFIYASEADILNVSLFGITAKEWRDENPDLKGNIRDYADISQ
                     LVCLSNLENLNAVFINEGMSQSERLEKLNAIAIEQMKILSESESIKKLK"
     gene            complement(28521..30227)
                     /locus_tag="PC407_000097"
     CDS             complement(28521..30227)
                     /locus_tag="PC407_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDF2495584.1"
                     /translation="MKLVIAEKPSVAISIAKVIGANKKKDGYYEGNGYRVSWCVGHLI
                     QMANPDAYDEKYAKWNMADLPIIPSDYKYEVAKATKKQFNILKKLMNDKEVDTVINAC
                     DAGREGESIFRLVYNQVNCKKKMKRLWISSMEDSAIKEGFDNLTDGKDYDNLFESAQA
                     RAIADWLVGMNISRLYSCLYQQNYSVGRVQTPTLAMIVKRDDEIANYQKEKYYTVELS
                     MNGFTLSTDRIDDEVAAEQLISLVGDKIEITDVIQKEKITKPDLPFDLTTLQRECNKY
                     FGYSAKQTLDYTQSLYEKKLITYPRTDSRCLTEDMIVSTVNNILGKNDFDTERIKVVF
                     NSKKVTDHHAIIPTISSVKEDVSELPLSEAKVYFLISDKFHASVGYPLIENTTKIVAS
                     FDGFEFTSSGKVIKDEGFSKYLKEYKSKKSEDAVLPDVSVGDVLSVENKEVKEKFTQP
                     PKHFTEDTLLKSMEIAGNDALEKGVEVERKGLGTPATRAGIIENLIFKRFVERDKKNL
                     IATHKGISLVTIVADTFKSAETTAKWEMELSDIAQGKSSKKEFLDAIENEIKEAVLTY
                     RK"
     gene            complement(30322..31863)
                     /locus_tag="PC407_000098"
     CDS             complement(30322..31863)
                     /locus_tag="PC407_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HDF2495585.1"
                     /translation="MKTSLKKNNKFWTVALAVIVSISCILGLWTVVYAQEQKSEAPTK
                     NEAVQTEVEVNVRYIFEDEKVFKEEKIKAEKGQLIDSGDLPMLPDDMKFIDEFLFYEV
                     KGDGKDEIIRKVAKMEVKDKETQTEEEKPKQDECTQTEDKGTQTELSKDDISKIEKEA
                     KELQDKLDKLNCELKDKDKLSDKQKNKIKDLEDEIDSLKEKMKKDKGNKDLSEDMKKE
                     IDKLTEKVKELEKKATETNKAPVTSQSVTPISPISGIKTSSGISPQTPQTSVSSTGKG
                     SSDSVSSGNTTKDTGKGKTEVKEKEKEVRYPNKLTAKAPANNSQDSSMGSASKSVNTN
                     KGVASSPSKARASVTENKDNANKEYPIHHGDSSDNKETDQYSADARQFITFQTKNGKT
                     FHLIINHDEDSENVMLLTEVSEDDLLNMVEKKEAPKQEVVKEEPVKEEVKPEKKDEKS
                     NLGTYIILLLVVGGALGAGYYFKVVKKKEDKELEALEEEDDDFFSESESEEEINDADE
                     VEDEE"
     gene            complement(31838..32077)
                     /locus_tag="PC407_000099"
     CDS             complement(31838..32077)
                     /locus_tag="PC407_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2495586.1"
                     /translation="MKKELTTVKNRIKKLKDKKALIDEELEPLFIREEELENEEIIAI
                     CRKNNITISDLIAKVNRQKAEMKKEKTNENQFEKE"
     gene            complement(32089..34221)
                     /locus_tag="PC407_000100"
     CDS             complement(32089..34221)
                     /locus_tag="PC407_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDF2495587.1"
                     /translation="MGKKLKKDFKERHKASLEREMFHSKTDTIPEESKLKHNDDYRGK
                     IVHEKERFQDKVHEKTSKVSADNEKSYGTSKRNEKYRTSDKGDVNSAIETGRSDYITE
                     VKDGKIYDPLGKDLDNDGIIDRYDNDFRDSNYFESTYDVEDNLSHKEKSLENSFKNHK
                     TQKSRYKRKNYSESLYTRKKDDAPKGNKADDKKAGKDAVSGKVYSSLSKDQKKKLKKD
                     MVKVSALSGLAKGSETVRDYLSHGSDENQGVEAGEKTADTSSKLIHGIKKYSDKKRAK
                     RGYDLTNKDYKIRKRKSKLEFRDAKKELKKTDEYKRASAYKRFQKKNQVKAAISRENK
                     SRLRDRIKEGLIGTLKSSKDMIIRKAKGLMLIFIGIIILGTFVINFAGTGMTGFMNST
                     SSVLTTSYLSKPNVLSEINQKFSDMEGELQSEVDYVKENYPGYDEYILNNTEYIGHNV
                     HELLSYITSRCGEVKSVSEVESILKELFESMYDLEYREEIEIRYRTVTETYTDEDGNE
                     YTESHEEPYEYKKLIVTLHKKEMDSIIRKVFANYPDNLKHYEALFLAQGNMREAFGNS
                     DLISANGGIGGGKEYEASTEVQKKIVNAAYITPSPGAGWCAMWVSQVYQNAGLGYIGG
                     NACDMYRNYTFTSDRSKLKVGMLVAVESSSSGSSAGLTYGHVGIYIGDGKVIDNIGRI
                     RVTTLDDWIATFCKHHPVGFGFPPNVKK"
     gene            complement(34225..36654)
                     /locus_tag="PC407_000101"
     CDS             complement(34225..36654)
                     /locus_tag="PC407_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2495588.1"
                     /translation="MNKKRKIQQIKLMQNEKALRSQKRDLKKSNCKSKKDKGGILDLI
                     FKKEPKRYTVEDTIPYLRLLKSGICQLDEKHFSKSIAFQDINYQLALDEDRDLIFNQF
                     ANFLNSFDPSISIEFSYINQLGRNEEMKSAIQIPDKKDGFDDIRFEFREMLKSQIVKG
                     NNGLKKSKYVTFTVEADNLEQATSKLERLEIDILSSLKSMGVRAESLNGEERLKILHD
                     VLNPNKTFKFSYKDLKKRESTKTYIVPDEFNFTPSRYFKFGKFIGATSHFQILASELS
                     DRMLSEFLDIDDNINISFHIRAIDQSEAIKMVKRKNTDIDKMKIEENKKAVRSGYDMD
                     ILPSDLITYGEDVKSLLKDLQTRDERMFVVSIVFMNFARTVQKLDNTIAQISSIANKH
                     NCKLKRLDHSQEQGLVSVLPLGVNKIEIDRGLTSSSTAVFMPFTTEELFINSSNSLYY
                     GLNALSHNLIMADRKKLKNPNGLILGTPGSGKSFSAKREMANAILVTDDDVIICDPEG
                     EYGNLVRQFKGEVIKVSSKSKDYLNPLDINMNYGDGDAPLKDKANFIMSMLELVVGGS
                     GLTAEEKSVIDRCLPKIYEKYFEHPTPDNMPILQDLYDMLKGQEEKVGKKLATEMEIY
                     VSGSLNVFNHRSNVDLNKKLLCFDIKELGSQLKKIGMLVIQDQVWNKVSQNRGNKATR
                     YYIDEFHLLLKDEQTASYSVEIWKRFRKWGGIPTGITQNVKDLLMSKEIENIFDNTDF
                     VLMLNQASGDREILARKLKISLPQLRYVTNSNEGEGLLFFGNTIVPFIDKFPKDTILY
                     QKMTTKPEEVR"
     gene            complement(36566..36955)
                     /locus_tag="PC407_000102"
     CDS             complement(36566..36955)
                     /locus_tag="PC407_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDF2495589.1"
                     /translation="MAYVPIPKDLNRVKTKVAFNLTKRQLIGFTLAGLVGIPVYLFMR
                     KFVPNDIAVIFLIVSTLPIFFITLFEKDGLTFEKYFKYIYLHKFYQPQKRVRKEVYLE
                     QEKKNSANKTHAKRKGIEKSKAGLKEK"
     gene            complement(36959..37219)
                     /locus_tag="PC407_000103"
     CDS             complement(36959..37219)
                     /locus_tag="PC407_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2495590.1"
                     /translation="MNKRKTVFIVVIGAGIMAIIDRIRLHNKVEELEEKTEDIGRCHN
                     DFYLIQQRYNKNTDEQLATIQEEIGSVYEHFEELSKGKEDGR"
     gene            complement(37237..38100)
                     /locus_tag="PC407_000104"
     CDS             complement(37237..38100)
                     /locus_tag="PC407_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495591.1"
                     /translation="MFGIFDKIEEFFKELLLGGIQANLESMFLDINDKVGAVATDVGK
                     TPMGWNGDVFAFIKSINDSVIIPIAGLIITAVLCIELINMVMQKNNMHDTDTFEFFKY
                     IIKMWIAVWLVSHAFEFSMAVFDVAQHMVNKAAGVINTSAVISGDQIVKMVDGLKDKG
                     LGELVMILFETSLIKVAIQVISIVIMLVVYGRMFEIYVYSSISAIPFATMGNKEWGQI
                     GTNYIKGLFAIGLQGLFLMVCLGIYAVLVKTIQITDIHTSTFTILGYAVLLGLMMLKS
                     GTLAKSVLNAH"
     gene            complement(38120..38335)
                     /locus_tag="PC407_000105"
     CDS             complement(38120..38335)
                     /locus_tag="PC407_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HDF2495592.1"
                     /translation="MEFFAQAVNVLKILVMAVGAGLGAWGVINLMEGYGNDNPGAKSQ
                     GIKQLMAGGGIVLIGLKLIPLLANVLK"
     gene            complement(38339..38650)
                     /locus_tag="PC407_000106"
     CDS             complement(38339..38650)
                     /locus_tag="PC407_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2495593.1"
                     /translation="MKQEMININANLVAEPTFSSFEKEGEEVEVVNFTLVKKYGKGKE
                     YINCAAYGEKAEKAEDFEKGDLIHVFGYFKKREKEGKTYKNFVVKSYNKIEKKEENEE
                     E"
     gene            complement(38814..39047)
                     /locus_tag="PC407_000107"
                     /pseudo
     CDS             complement(38814..39047)
                     /locus_tag="PC407_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
     gene            39189..39362
                     /locus_tag="PC407_000108"
     CDS             39189..39362
                     /locus_tag="PC407_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2495594.1"
                     /translation="MDYMTLKEASEKWGVSSRQINYYCTDGRIPGAVKMAGVWLIPKE
                     AEKPKDRRYNESR"
     gene            39367..40077
                     /locus_tag="PC407_000109"
     CDS             39367..40077
                     /locus_tag="PC407_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2495595.1"
                     /translation="MLHIAICDDEKDFVQYLTDLLNQYSKETGRDIKITPYYDGMELI
                     EKYDPTIDLIFLDIQMKVVDGLHAAERIRQMDETVGIIFLTTLTQYGLEGYKYHATNY
                     IIKPMKYARLKSEMDKFLERSQKEDTPSLVITNDTGKYKVPLKSIRYVETYNRNLMFH
                     TEQENIICYKSMKEVERELCDKDFVRCHTSYIVNLFYVKGIKKLDIELITGEIIPISQ
                     PKRKEFMERLTDYWGDML"
     gene            complement(40348..40509)
                     /locus_tag="PC407_000110"
                     /pseudo
     CDS             complement(40348..40509)
                     /locus_tag="PC407_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569186.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            41120..41305
                     /locus_tag="PC407_000111"
     CDS             41120..41305
                     /locus_tag="PC407_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2495596.1"
                     /translation="MLKKYWIKCPICNGKTRVQVFHNTVLKDFPLFCPKCKLTHIIDV
                     EKLEIVIKNTEKQTFII"
     gene            41322..42305
                     /locus_tag="PC407_000112"
     CDS             41322..42305
                     /locus_tag="PC407_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDF2495597.1"
                     /translation="MKRLPKYTPAEVRNDPYGFTYKEMSEVIGENEAKALYEELYKQL
                     PRKKNLSMLVKNICKSSDTEKYVYELKDNKYIETVFIKRRDGGTVCVSTQVGCPVGCI
                     FCESGRNGFVRNLTSSEIVQQIILLRRKVNRIVFMGMGEPLFNYDNLIKAIHILRDRY
                     GLNFPTDGITISTVGPVDQLKKLREEHLKIQLTISLHAATQSARNRIIPHMRIYAIED
                     VVKQALSYSERHNRKIVFAYLLLPGINDRPSDVRQLAKWFRGKKVMINVLQYNPTSNS
                     RIKAPQKREIVAFKHQLEQAGLEVTMRVSHGREINAACGQLANTYNKFKKK"
     gene            42779..43216
                     /locus_tag="PC407_000113"
     CDS             42779..43216
                     /locus_tag="PC407_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2495598.1"
                     /translation="MELSSSDKERIQHQYDALAKKTLVGEAKSHRRTLAKRAAREVTF
                     SDLSESELAQLFTTDEYESDYFRFQVSGFDVLVKNELLAEALNALPERKRDIILLSYF
                     LDMSDAEIGELLNVVRTTVFRHRKSALAKIKQYLEGKADDEYR"
     gene            43200..43442
                     /locus_tag="PC407_000114"
     CDS             43200..43442
                     /locus_tag="PC407_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2495599.1"
                     /translation="MMNTVRKSENLLPFPVISAAANGDTTAMCAILKHYEGYIAKLCT
                     RTLKDDAGNTYSYVDEEMRNRLQVRLITRTLAFHVG"
     gene            43901..44074
                     /locus_tag="PC407_000115"
     CDS             43901..44074
                     /locus_tag="PC407_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-encoded TnpW family protein"
                     /protein_id="HDF2495600.1"
                     /translation="MDKKQTITTERKIGKITYLVQALPSEKATDTIHKKIEKLIVKDL
                     QKKPGNPGFLASE"
     gene            44152..45825
                     /locus_tag="PC407_000116"
     CDS             44152..45825
                     /locus_tag="PC407_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2495601.1"
                     /translation="MLQSNKITALYCRLSQEDMQAGESGSIQHQKMILQRYADEHHFL
                     NTKFFVDDGFSGVSFEREGLQAMLQEVEAGRVATVITKDLSRLGRNYLKTGELIEIVF
                     PENGVRYIAINDGVDTAREDNEFTPLRNWFNEFYARDTSKKIRAVKQAQAQKGERVNG
                     EYPYGYIPDPNNRHHLIPDPETAPIVKQVFAMFVSGVRMCEIQKWLAENKVLTIGALR
                     YQRTGQARYQRAMIAPYTWPDKTLYDILARQEYLGHTITAKTHKVSYKSKKTRKNEEE
                     QRYFFPNTHEPLVDEETFELAQKRIATRHRPTKAAEIDIFSGLLFCAGCRHKMYYQQG
                     VNIEPRKFSYSCGAWRNRARTGSECTSHYIRKNVLLDLVLEDMRRVLRYVKEHEQDFI
                     CKATEYGDMEARKALAQQQKELFKAQARMTELDTLFRKLYEDNALGRLTDERFVFLTS
                     GYVDEKKSLAARIDELQQQIATVTERKRDISRFIQIVGKYSDIQELTYENVHEFIDRI
                     LIHELDRETNTRKIEIHYSFVGQVDTEQEPTQVVNHDRRNMVDVKSIAI"
     gene            45849..46304
                     /locus_tag="PC407_000117"
     CDS             45849..46304
                     /locus_tag="PC407_000117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495602.1"
                     /translation="MKEYTLIIKGEMDFIILSPQVLSSLITQIHNSPERKVVVSIESI
                     MPPKFTDYLLRVINSNRFSNERFRYRYILENPVTKKGMYEILRQQLSRTNTERFPCFQ
                     TIQLTDTFQGNVELDMECNDLFFWACKDTAAKFVYTFPDGREETLVIEY"
     gene            complement(46352..48004)
                     /locus_tag="PC407_000118"
     CDS             complement(46352..48004)
                     /locus_tag="PC407_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2495603.1"
                     /translation="MSRKKQVYEKITALYCRISLDDGGDNESMSISNQKLMLRDFAEK
                     HGMFQYEYYVDDGYTGRNFNRPSFQRMIADIEAGKIGCVITKDLSRLGRNYIEAGSYI
                     EIFFPKHNVRYIAVTDGVDSLTRQEMDITPFKNILNDMYSRDISKKVLAGRMTRSRQG
                     KFCGGQPPLGLMRDPEDKGHLILDPETAPVIRKIYDMALDGWGCMRIAKQLMDDKIPI
                     TRVKSNTECDVNYYSWGSARISHILRNPFYKGAHLVCRTHQKGIRSNTYDIIPREDWE
                     VIEGCHEAIVTPEEWEQVQDIIDRRPTIMKGNACPFYNLFHGLVYCATCGKSMQVRYE
                     KVGRTGKNRFTGEMREPIDRAYYICQTYNRLGKNACTSHKIEARDLYNLVLKDIQELA
                     AQAMKDADAFYQRLSSRMERRYLVDASQTEKERKRLEDRNQEIDGMFLSLYTDKAKGV
                     LTEQRFMKLTAALEQEQEANQKRLHDLAVMQSRADAQESEVRTFIKEIRRYAAIAELD
                     ESVLNRLISKILIGEVKKVDGQKVQEVRIVYNFVGEIPEIAA"
     gene            complement(48077..48244)
                     /locus_tag="PC407_000119"
     CDS             complement(48077..48244)
                     /locus_tag="PC407_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495604.1"
                     /translation="MEKSKNQKNKVRFVVVREFSGEKSMREAFEQLIERQTCEHFEEW
                     RHQREMAEKAA"
     gene            complement(48512..49870)
                     /locus_tag="PC407_000120"
     CDS             complement(48512..49870)
                     /locus_tag="PC407_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
                     /protein_id="HDF2495605.1"
                     /translation="MSAAEALAPEQSVDEVREGLSVTEKGQPANTIGNCRTVFSHDPL
                     LRDAIRLNLLTDRVDIVRDLGWRRNTSALTDTDVKYLLLYFEQNYGLTSEKKMTAALS
                     IVANENCYHPIQDVLNGLVWDGTPRIRSCLHHFLGAEVSDYVEEMLKHFLLGAIRRVF
                     SPGSKYEEMLCLVGGQGAGKSSFFRLLAIRDEWFSDDLKKLDDDRVFLKLQGHWIIEM
                     SEMLATSSAKSIEEIRSFISRQKETYRTPYEAQPKDRLRQCVFGGSSNTLDFLPLDRA
                     GNRRFLPIMIYPEKAEVHILEDEDASRAYLLQVWAEAMTIYRSGKYSMKFSKSIQRQL
                     VEVQKDFMPEDTEAGQIQGFLEHYTGSMVCSKQLFKEALGHTYDEPKRWQLHNINEIM
                     NTVVTGWKPFSNPRMFAGYGRQKGWERDVSGNELPGNEDGFVELSEEECRQLELPKEW
                     IA"
     gene            complement(49830..50516)
                     /locus_tag="PC407_000121"
     CDS             complement(49830..50516)
                     /locus_tag="PC407_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDF2495606.1"
                     /translation="MKTGCLFLCLEVRKSNVFEAVKQAVPTRQAAESYGIQLGRNGMT
                     CCPFHDDKHPSMKVDRRFHCFGCQADGDVIDFTARLFGLNKKDAALKLAEDFSVGFDA
                     KGHDPPRRKPGKRKISEELRYRQAEQKCFRVLCDYLHLLERWEKEYAPQTPEEAWNPL
                     FVEALQKKPYTEYLLDILLSGSMEERACVVAEYGKEVRKIEQRISEFTASHPAGRHER
                     SRSFSAGAER"
     gene            complement(50532..51776)
                     /locus_tag="PC407_000122"
     CDS             complement(50532..51776)
                     /locus_tag="PC407_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/arginine repetitive matrix protein 2"
                     /protein_id="HDF2495607.1"
                     /translation="MKLTRHNGRSGKHGTYNPRHNDRRFDVENSEHIDAERARQNVYW
                     DCYRGFTTHEFRENPEQPDFSFEEIERMYYNEHYGGHVEAQNARNEKTRHTERNRTVE
                     DLLKNNKTCPEESIYQIGTMGESVPPDTLFSIVNEFYEEFERRFGSHIHILDWALHLD
                     EGTPHIHERHVFDCENRYGELCPQQEKALEELGIPLPNPEKPKGRNNNRKQTFDAVCR
                     TILFDIARRHGLHLDQEPSYGGRDYLEKQDYILMKQKEQLAAQEQKLEELTLKIEDVE
                     TLLDDVSDAAYDKAVEVVTDTVRQETHKEDIRLVEESKKWVLSPERKAPKKEREYAAE
                     RLDGVITKIKNAMQHALAKIQRTLMQPEVKQAGKEQVKKKAKESIMDILAKAKINADR
                     DNRERWEREGRIAPTKKNDIEL"
     gene            complement(52204..52509)
                     /locus_tag="PC407_000123"
     CDS             complement(52204..52509)
                     /locus_tag="PC407_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDF2495608.1"
                     /translation="MRKLSKYEKETIINWNEAENLASVYTFNASLKRRLADFSRKYPL
                     LCRLERSTPEGSVTYVLDKSRLSIRLVPPYSEERKAAASAYAKEHGFQVVGAEEKIA"
     gene            complement(52493..52786)
                     /locus_tag="PC407_000124"
     CDS             complement(52493..52786)
                     /locus_tag="PC407_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495609.1"
                     /translation="MTLSMKEKKILYAYGCPSHHNTVTRLKWLTALTVDPEAKRRMLG
                     LARKVETEVGENWYEDFYHHLRMEMDEYRRLKRSLRVLKSYTDYEEDLYEEAV"
     gene            53152..54000
                     /locus_tag="PC407_000125"
     CDS             53152..54000
                     /locus_tag="PC407_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2495610.1"
                     /translation="MYAKLTIPERLKDLRVVDKHLTLEQLAEQTGLSKSALGKYETDD
                     YKDISPFAIATLADFYGVSTDYLMGLTENKNHPNIELQSLHLSDDMVELLSSGKINNR
                     LLCELATHPNFRRLMIDMEICIDQIANMRVEQMNLVLEATRQTVMNKYAPGEDDLYMR
                     TLELGQVQESDFYSHVMHDDLDSIVRDIREAHRKDKTTADPQPTLDDVKEKFEQAIGQ
                     GSDTEMLIHEFCDKLQIPFEKISSEDFSAFLRILSLSKQLKSSNNMRGKASPIPPNRK
                     ARRKRK"
     gene            complement(54122..54403)
                     /locus_tag="PC407_000126"
     CDS             complement(54122..54403)
                     /locus_tag="PC407_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495611.1"
                     /translation="MILEEMYNGRFYPCETVVADSPRFKQAVKASAALMDTLSERLSK
                     EDYALVEELREQVAIAQCEENESHFKYGFSAGLLVQQEAHAQVSQKDKE"
     gene            complement(54417..54794)
                     /locus_tag="PC407_000127"
     CDS             complement(54417..54794)
                     /locus_tag="PC407_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HDF2495612.1"
                     /translation="MEHLPKKIHHNGISYTLVGDYYIPDLKLSEEIRPIGRWGRIHKA
                     FLQEHRPGQYNELLLSGKLWTYLADLNEQADDRLACIISQMQAAEGVTEELKARDQLA
                     WVGGMNSIRSRVEEIILSEMIFV"
     gene            complement(54892..56454)
                     /locus_tag="PC407_000128"
                     /pseudo
     CDS             complement(54892..56454)
                     /locus_tag="PC407_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
     gene            complement(56451..56936)
                     /locus_tag="PC407_000129"
     CDS             complement(56451..56936)
                     /locus_tag="PC407_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HDF2495613.1"
                     /translation="MINEEIARKTLNMEINAGKVTGKVLLNLLKKLMKEAEKFGGLEK
                     LVNANGNEVKLKDMVKKGQLEEIPVEEAELKELKKELNRYGVKFSVMKNKETGKNSVF
                     FQAKDMKVMDKAFKHALSKSEKKAERKESIHKNIEKFKEMAKNSISKEKIKNKQKEQS
                     L"
     gene            complement(56938..57960)
                     /locus_tag="PC407_000130"
     CDS             complement(56938..57960)
                     /locus_tag="PC407_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2495614.1"
                     /translation="MYLFDEQPIVANKTLAREIGLNEALILQQINYWIEINKKSGKNY
                     HDGKYWTYNSIRAWQENDFDYMSFDTVKRTFSKLEKAGYLLVGNYNKDPRDKTKWYTI
                     NNDKLEELYMDMDLKKQKIQTEALKNSMPNALGQNAPMDKGKISQCNNADCTNAQVQN
                     ALMQKDNLHQPLPENTTNNTTNNTTNNSAYISIHPSIKEKNQLFSENMKGGRIDISYK
                     NIYQNCLEELRHQTGYYEHMSIGNKSVAEEFDEILKILADVMVLDDESFITINQTKIS
                     VQVVKERFRKLNSGHLEYLVGVLRENEYRIRNIRAFVLTAAYNAPSGIDAYYSALVSY
                     DMRGGY"
     gene            complement(58046..58327)
                     /locus_tag="PC407_000131"
     CDS             complement(58046..58327)
                     /locus_tag="PC407_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495615.1"
                     /translation="MRWILKIILFPISIALSILIAFLSFLLGIGTAILYLLMMFCIFG
                     AIASFLQKEVTIGIEALILGFLLSPYGIPMVGTAVIAVLTGVKEAIRSI"
     gene            complement(58346..59980)
                     /gene="rlmD"
                     /locus_tag="PC407_000132"
     CDS             complement(58346..59980)
                     /gene="rlmD"
                     /locus_tag="PC407_000132"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2495616.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAEGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVALLS
                     KLDVDKHIDVEIKLDELDLTSAESKATYAQIKEYVLEKFGLKVSTLYIAQIKKKCGIE
                     LREHYNKSKKDNQAIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(60155..61915)
                     /gene="pyk"
                     /locus_tag="PC407_000133"
     CDS             complement(60155..61915)
                     /gene="pyk"
                     /locus_tag="PC407_000133"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDF2495617.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEILTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(61967..62926)
                     /gene="pfkA"
                     /locus_tag="PC407_000134"
     CDS             complement(61967..62926)
                     /gene="pfkA"
                     /locus_tag="PC407_000134"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2495618.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(63135..66704)
                     /locus_tag="PC407_000135"
     CDS             complement(63135..66704)
                     /locus_tag="PC407_000135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDF2495619.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(66861..67808)
                     /gene="whiA"
                     /locus_tag="PC407_000136"
     CDS             complement(66861..67808)
                     /gene="whiA"
                     /locus_tag="PC407_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDF2495620.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(67825..68250)
                     /locus_tag="PC407_000137"
     CDS             complement(67825..68250)
                     /locus_tag="PC407_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2495621.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(68319..69425)
                     /locus_tag="PC407_000138"
     CDS             complement(68319..69425)
                     /locus_tag="PC407_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDF2495622.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(69437..70294)
                     /gene="rapZ"
                     /locus_tag="PC407_000139"
     CDS             complement(69437..70294)
                     /gene="rapZ"
                     /locus_tag="PC407_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDF2495623.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIDEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(70325..71038)
                     /locus_tag="PC407_000140"
     CDS             complement(70325..71038)
                     /locus_tag="PC407_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2495624.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYNDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFKLA
                     MKTISDSNLDISLVENIII"
     gene            complement(71042..71956)
                     /gene="murB"
                     /locus_tag="PC407_000141"
     CDS             complement(71042..71956)
                     /gene="murB"
                     /locus_tag="PC407_000141"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDF2495625.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(72229..72999)
                     /locus_tag="PC407_000142"
     CDS             complement(72229..72999)
                     /locus_tag="PC407_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2495626.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT        20516 a        15617 c        10917 g        26051 t
ORIGIN      
        1 gaaaactatg tagttttaat tataaaaaaa gatgaacaaa aattatacag tttaatatgg
       61 aatcagtagg tacatatgta ttattaagaa ttataaataa taaaaataaa ttttagtcaa
      121 tctacaaaaa ccttttaaat tgtctggaaa tcacctcctc atttgttata aaaacataca
      181 acaaatgaag aggtgatttt atggttttta aaaaaataat tataagtgga tatgtgcttt
      241 cacatgttta tcaacatttg taatgcctat atttgctaat gaattataat ataaaaatat
      301 taaataatat tcaactatag attatgtaaa ttatcctatg aaaaaaataa gttgtataaa
      361 atctggcaac tgtagattga cgatttcttt taaaattaca attataataa gttttataga
      421 attaaaaata aaactttaga gatttttgat aacattgtag actaaatttg ccaccgaaat
      481 tactttacag tggcaaatcg gtggcaaata caatatatta ttagtatata atctatataa
      541 acattactat tgcttgatta tcttagctac agtttcaact tgctcatcat tttccaaact
      601 tatattaaga tcttcatcaa tgataggtag cttaaaagta atggatttta gccattgtcc
      661 attaggctgc ttttcttcat aaatttgaat ttctgaaatg agtgcttcta ttaactgcct
      721 gcgttcaaca tcgttcatga ctttataaag cttgtcaaaa taaatcaata ctttatatat
      781 gttatcacca gtaagtttct caacctctat cgtttctttt tttgctttag cttctattaa
      841 ctgactttct aattcctcaa ttttatcgta catacgataa agcctatcat ctaaatcatt
      901 ttttcttctc ttataatgtt tatcctctac atctaaatta tctatttcct caataagctt
      961 atactttgta gaatgacttt ttcttagttc attttggtaa ttatctatct cattttcaat
     1021 agcagatgta tcaaccttca tgttaatttt ttcttgtatc atagatgcaa atttagggtt
     1081 gcttactaac ttgattatta cctcagcaac ggcatcatct aacaattctt ctctaatttg
     1141 tttattatag gtacatttat gtcctctatt cataagtcta tgcttacaac catagtaata
     1201 gaagtcttta tacttcgtcc catctttttt atattttata gatttattcc caaacattcc
     1261 agctccacat atcggacact ttactattcc tgataataag tgtgtcctca tgttcttgcc
     1321 cttatttaca tgttcatact ttttagcttg tgctttcagt ttaacttgtg cagcctgcca
     1381 caattcatct gggattattg cttcatgaat gccttctgct actaaataat cgtcctgctc
     1441 tacttgtctg tattcattcc ttgtaccatg aactttttct aaagttcttc ttccaaatgc
     1501 tattttcccg ttatacacag gattctttaa tatttttctt ataagacctg cgtcaaacaa
     1561 tggatttttc ccattttgtc ttggtatttt ccttatacca tgattttcta aatatttaga
     1621 aagaccatta gctccaatac ttgtatttac atattgatca aaaatagttc ttattgcaat
     1681 tgcttcttcc tcatttatta tcaatttccc atcaataagt tgataaccat acggagcaaa
     1741 tccaccattc catttaccct cttttgcctt ttgaatgcga ccttccattg tttggacacg
     1801 aatgttttct ctttctattt cagccactgc cgataaaaca gaaatcatca atttacccgc
     1861 atctttagat gaatcaattc catcttcaac gcaaataaga tttactccaa aatcctgcat
     1921 tatttgtaaa gtcgaaagta catcagcagc atttcttgca aatctcgata gtttaaatac
     1981 aagaacgaaa gacactccat cctttccgga ttttatatct tccatcatac gagtaaactg
     2041 aactctacct tctatagatt ttccagactt acctgcatct tcgtattcac caacaatctc
     2101 ataatcatta tagagagcaa aagccttcat tcttgatttt tgtgcctcta aagaataacc
     2161 atctatctgt atggatgtag atactcttgt atagagatat acttttattt tttcttttga
     2221 catagcatta acctcaatat ccttattata tatttatata tttttaccaa taaaggtttg
     2281 gactttaatc tcaagtatat tatagcactt ttatttctct ttctcaatcc gtgcttttag
     2341 ctatatcaaa tttattctta tcctctgtat tttttactgg agtaggattt ggaagattct
     2401 ctaaatctaa aactccagca tattttgtaa tcagatttgc tatcaaatca gccaatccat
     2461 tccagtcatt atccactaat acacctcctc cactttaaat tctttataac caaatattat
     2521 ttttcttact aagttttatg acattggtag gtgcattggc tgctacatag gaatctcacc
     2581 tccgcccctt gtttcagacg aggcagctta aactattgaa gtatcattat caccacttac
     2641 atcatcgaaa taagctgcca cgcttatctt ttatgtattc ctatctatcg ctcattttct
     2701 tctaacctcg gcattatgcc ggtattcatt tgaaccgatt ttcatcagca gaaaagtctt
     2761 ggcgtaggtc ataactctca gtaagtagat aaagtcctac cttggtctat tcagttgtca
     2821 aagatcaata tttgaaaggg actgttttct ttacattttc ctatttgcca taagcaggcg
     2881 tatctcctac tcaagaaagt aaaggaggtc aaactcccct tcactttaca taaggaaaat
     2941 ttgccccctt tggttaccaa agttttaaag ttcttcgata aattttttca gcttttcaag
     3001 aattttgttt cttcttttga ttaaagccgg atgtgtaata cttttgagct ttgctactga
     3061 acgaacagtt tcatcattga aatacaagcg ttcaataata tctctttctt ctgcattgag
     3121 ccttgttata gcacttctta ctgcttcaat catcatctgt gtttcaacaa tcttttctac
     3181 atcaacactt tcatcaacaa tattatctgt aaagtgtccg tcatgatcca atgctgaaaa
     3241 aaagagcaag tggtttttcc tgtccacctg ctctaaatac ttctcgtgtt ctttttctcg
     3301 ccagtagact atatatatat cttcactgac ttttaccttt tgtcctctga tataaagata
     3361 atactctttt gccataaagt ttcctccatc tcttttttat ctgcttgttt ttcagataaa
     3421 aaaggaggac ttctgcatct tggcataaga agtcctctga aatgaaagaa taaaaatatt
     3481 tctttatatg aacttgattg tttcaattcg cacctgaaac aatcatctac tatacatatt
     3541 ataaaaaaga ggaatgtatt gaattctaaa tatttctaac attaaacgtt tagatgcttc
     3601 catatttatt tcctccattt tatcaataat ctaaataacc atcatattca tctactactt
     3661 atataattga gttttattaa actttttaag tttctattct atcaaatatt taaacagtct
     3721 ctgcatccga aaagactaac tcatatagtt ttactaacaa tattttcatt agcgtgttca
     3781 aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa aagcctttac
     3841 ttccatggct aataataagt ctctattttt caataactat tgcaaaatta tatcttgttt
     3901 aagaaaatat tttaatttta aagataaaaa attcttcatc ctgcaatgct ttatattcta
     3961 tattgtaatt attcagaata ttacttacaa tatataaacc taatccatta ctattttcct
     4021 tatttaaatc aaatttaaca tcaaatattt tatccatatt cgaaatttta ttgttaccgt
     4081 atgaattctc tatatataac caatcattaa ctatcccaat attaattacc ccatttacat
     4141 cagtatattt taccgcatta ctaatcaaat tagatagaat aatctttaaa gctgtttttc
     4201 ctatataaac attctcatct aatatataat tattgacagt tattttcttt tgatttgcta
     4261 atatttcata cttttttaat atgtcttcta atgtatcatt tattttcaaa tattcttcat
     4321 cattattcaa attttctata gaatgaactg ataatatttg agaaatattt tttgttaaac
     4381 tatcaacgat ttcaatacat tcattaatat aaatatctct ttctttatat tttccaatat
     4441 tgtatttcat gttttccagt attattttaa gactagcaag aggagtttta agttcatggg
     4501 atgcaccttt aaaaaagtca tattttaact tttctaattt taaaatttct ttatttttaa
     4561 attccaaatc gtcaattgtt cttaataaag tagaatacaa atcattaatt tgttgtttta
     4621 attctccaac ttcatcatta gagctaactt ttaaacgcgt ttttttatca agtttcatca
     4681 ttttatcagt aacaattttt atttcttgta tattattttt tattgatttt gcataaatta
     4741 aagaaataat agcagaaaat aaaattgata ttaataaaga atatggtaaa aatttaaggc
     4801 ttaaatcttt cgcatctttt tgcatatcag ctgtagaaac aaattgtaga tttgtttttt
     4861 taccatcatt aagctttatt tctctttctt caattatcaa agaattacta tcactttcta
     4921 aattaacatt tatattatct ttgatttgta tttcattctt attatttttt tcttttataa
     4981 atgcttttat ttcactactc ttagaataaa gctccaaagt ttgttctata tattttattt
     5041 cttttccatt catattacta gaaatttcat ttgccatatt atgaatcttt tcttttctcg
     5101 tctccaaata tgttttagga aagataaaaa aaactaatga atgaactaat ataattatta
     5161 ttccaagaac agaaaatatt tgtatgaaca tttttggaaa tatctttaat tttttcattt
     5221 tctctccaat ttatatccta catttcttat agtagttata cagtctaatt gtaatttttt
     5281 tctaagttct tttatatata catctattac tcgatcataa ggagtttctt cgctatcttt
     5341 ccatacataa tcaataattt gcattcttgt taacacttgt ccattattat ccaataaaca
     5401 ttttaatact tctagttctt ttgcatttac atctatttct tcaccattta ttttagctgt
     5461 atagctatta aaattaaccg atagatcatt atattcaaac ttctctaaat gtccataatt
     5521 cttcttaatt aaagaatcta ttctagcctt taagaccggc aatgaaaatg gcttttctac
     5581 atatccgtct actaaattag taaatgcatc aattttatat tcttcatcac taaatgcagt
     5641 caagattaga attggtaaat tgcttttctt tctaatttct ttcaacactt ctaaaccatt
     5701 tataaaaggt atctgaatgt ctaagataac caaatttata tcgctattaa attttgacaa
     5761 ggcttctctt ccatctttag cttgaataac agtatatcca aattctgaaa gatattcact
     5821 tattccctct cttatcatat tatcatcttc aacagttaat attttcatat atacctccac
     5881 ttaagcctct acacttatat tatacagcat attatttgat atttaaaagc caaaaaattt
     5941 ttggtgctaa aaagcaccga aaattttaat tattgtcaat taataattct tttggattcc
     6001 ttcttaatat attgaatgaa gaaataatca atgatacaag aagtactaaa ctcataaata
     6061 taactacata aattatgaat ttaggtaata catttatatc taaacttgat aatgtcttgt
     6121 taaatccttc agcttctgcc ccaccaccta attggctaga tgcagattgt ctagctattt
     6181 gtttagctat atcaccagta accttattca atatattatt cccaagctta tcagctgtat
     6241 attgagctaa gaaataagaa ccaacgaatg cggggattga tataaatacc atctcaatta
     6301 ggaattgacc aaaaatttct aattttgata tacctaatga tagtaatact gctatttctt
     6361 tctttctagc attcatccat aagaataata ataatgaaac tacaactcct gcaaatatta
     6421 atgagccaac aaataattta tttgaaattg agtagatacc tgatatagat tgttgtaatg
     6481 cagggtaatt tgatgaactt ttaatcaaat tatattctct ccaatttata tctaatttcc
     6541 caagatcttt tattacacta tcaagattct tatcaccttt tacaaagaat gttgcatctt
     6601 ggtatacagc tgtatcttct gtattaccat aaactttagc agctgtatga acatcagtga
     6661 ttaatgtatt ttcgtatagt tcttgtgctg cacttactcc accgctatta tgcccatcga
     6721 atagaccttt aatttctact tctactgttt catctgcacc tttctcatta tcagcatcaa
     6781 ataaattaga ttttattttt atcttatctc caactttaag attatttttt tcagctaaat
     6841 ctttatgcat taagatttta tttttatctt cattttctaa atgctttcct tctactaatt
     6901 tatatgcttc tgatacaaat tttgtttctt ttgatgagtc gttaacccca gttaacataa
     6961 ctgttctttt aaaattcttc gctctttcag gtgattgatt cgcaagagtc tcttgagttt
     7021 caataatatc ataatcaact aaatctgcaa cactgtttat tctttttaca taggagtcta
     7081 tactatctgt ttgaaatatc tttttaatat cttcaccttt tacatttccc ccacctctag
     7141 gtgttcctgg atttacttgt ctatttatct ccatagaaaa actatttgtt atattagcaa
     7201 atgtttcttt tgaagctctg tccgtagcat ctttaataga taaactaatg agacttaaag
     7261 ttgacataga taaaataact aatataataa ttaatgattt taagcttttt ctagttacat
     7321 aagcaaatgc attttttatc attattcaac ctccaaattc attttattta cttttttcaa
     7381 ctttttacca cttagttcta aaatgatatc cgcagaatct gctacttcct tactatgtgt
     7441 tacaactatt acacatttat ttctatcttt agctaatgtc tttaatatat taattatttc
     7501 tccagcagta acactgtcta gattaccagt aggctcatca gctagtatta ttggagcatc
     7561 tgataccaat gctctagcaa tagctactct ttgttgctgt ccaccagata atttcataac
     7621 atttcttttt atttgttttt tatctaaacc tagttcaaac aagatacttt catctgctga
     7681 tttatttact aatctaatat tttcaattgg tgataaataa tctattaaat tatagttttg
     7741 aaatactaaa gatatattat tttttctatg attactatat cctttctttt gtatatcttc
     7801 attcttaaac aatatttttc ctgtttgagg tttatcaagt ccagcaagta aggaaagaag
     7861 tgtagatttt cctgttcctg actttcctac tatcgcataa aacttcccaa gttcaaattt
     7921 ttgatttact cctgacaaaa ctttttcttt agaatttgca taactatatg ctacattctt
     7981 tatttctaat atatccatta ttttctccta acttattttt gataatattt ctttaggctt
     8041 cttgattaat attaatgaag aagcaaatac aactgataaa acaataatac ttattaatat
     8101 taaataactt tgtccaagtg ttgttatatt taacataaaa ctactattat ttattaaact
     8161 tccaccggaa atcattgagt cctctgagtt aatgaatcca tctacaatta cttttagtaa
     8221 tacatttcct aaaaataagg aagatattat acttggtatc gatatgaata ttaactcgaa
     8281 tataaattgc attataattt gtatcttaga tgttccaata gataaaaata tacctatttc
     8341 ataaattctt tctcttaacc atagaatcaa gattaatgaa agaacaacca tcccacctaa
     8401 cataatagaa taagtcatta tttttattat atgttttatt ccactcactg actctaaaga
     8461 ctcttcaaac gcattagaat ctttttcaac aaaatacttt gactcatcaa ttttaagctc
     8521 tttcaatttg tttaaggcta agtctgtaga ttctgcacta ccagaataca ttaaaatttt
     8581 atttgcgatt ttattatttt ctgatttatt taatatctct tggcttgttg aataatctac
     8641 aaacaccata ttttcactaa aatcagaaga taatcctgta tatgtttcct gttttttacc
     8701 agaaaagatc cctataattt taaatttatg actttttatt tttccacttt tttcaatatc
     8761 tagtaattca agatcaactt catcacctaa ttttagattg ttttgtttag caaattcttc
     8821 atgaacaata attgaattct tatcattttc tcctatattt ttgccttctt taattgtaaa
     8881 tactccacta ctaaataaaa tatttctttt agtattattt gtagcttcga gtgaaacaac
     8941 attcttaaat tcgtcagata aatcttctct atttattctt tgttctccgc taactacttt
     9001 agcatctttt agttttgcta atccatcata ttgaattatt ttttcttcaa tttcttttaa
     9061 tttttcaata tctttaaatt gattaacatt aaaatattta ccatcttttt ttgttattga
     9121 tattgaagaa ttagaacttt catataaagc cttttctatt tcattgcttg atttcattat
     9181 tgttaaacaa gaatacaagc aagaaagaac tatagttaat atgataaaaa ttattaaagt
     9241 tctatttctc tttcttgtaa tatatgctat agcatttttt atcactttcc taactactcc
     9301 tctctatttt cttactccat aactaattgc atccactgga caagcatttt tacatcgtcc
     9361 acatctgata cactccagat gattgcaatt ctcaactgga tctatattca tttggcaaac
     9421 ttttttacat tttccacaat taatacactt atccttatcc aatctatatc taaatattga
     9481 aatagggtta aaaattgagt aaatagctcc aagtggacat atatatttac aaaaaggtct
     9541 atatataatt atagaaagta acaaagtaac tattagaatg attcctttcc aataatacaa
     9601 gaaacctatc gcatttctca tacttttatt taataaaaca agtggtaatc ctccttccag
     9661 cataccaatc ggacaaataa gtttacaaaa atacggagaa ccctgaccta aaatatcaac
     9721 caaaaataaa ggtaatatta ttacaaatac aagtaatatt atgtatttca attttctcaa
     9781 atgtttatca aacttaaatg tttcaacttt tttaaaaaag ggaatcttat gtagcaaatc
     9841 ttgaatcaat ccaaatggac acagccatcc acaaacaaat cttcccacca atgctcctat
     9901 aaaaaacaaa atcccaacta tataataact aatcttatat tttgaatttc caattactgc
     9961 ctgtagtgat cctattggac aagaaccttt tgctcccggg catgaataac aattcatacc
    10021 tggaacacaa attttcttta aattcccacc gtagatttta ccagtcttaa acccttcaaa
    10081 gtaagaatta gtaattaaaa accaaacgga ttgaaataaa tgcctaaaac gatctcttat
    10141 tttatattta tctttttttg tactaccatt tataatattt ttaattctat ccaattccaa
    10201 tacactccat acaaattctt atcgccttat ccaagacaac tttagtttct ccattactat
    10261 gaccatagta aatcatcgcc atactaataa agataattaa taaagacaag gcacggcttt
    10321 ttaaaaactt attaaccata taccaatcac cgctttactt ttatttattt tttatttttt
    10381 ccaattcttt ttcaattacc tgcttccatt cctttgaact ctttgcccca gaataagtat
    10441 cacctacaat atttccatta ctatcaacaa agaaagtctc tggcatagcc tgtataccat
    10501 tcaatctacc attaaagttt gtcttatctg gcattaagaa aggatatgaa gcctttgtct
    10561 tttcatgtat taactttgac ttttcaattg cttccttgtt ttcaccgtta tcatcaacag
    10621 tatctgtaac aactccaacg atatttacac ccttgctttt catttcattc tgaacctcaa
    10681 ccaaatcagg aatttccttt acacaagctg tacaccaagt tgcaaataca tttaccatag
    10741 taagatcata cttctcaaaa tctttacttg taaaatcctt gccatttatg tcctttgttg
    10801 atagtttacg taaatcttta accgattctt tgttaaatgc ttctgtattt tctaaatcag
    10861 ttttttctga tagcacaaaa ccatttttag gacgttcttt tttatctata atttgaatct
    10921 cagttctttt taattcatcc agaagattac tttcagctcc gctatttgtg cttaaatagc
    10981 aatcgtattt tccatcactt gaaactccta ttttagtatg agtatcacat tttgtaattt
    11041 tagatatttt ttcttctgat gtattttttt cgaatatacc aatagtccca attctttcaa
    11101 gctcattttg ccaattttta tatccatcgc ccatcttttc tataactgca ttcttttgtt
    11161 cttctgtcat tttttcaaat gtcaaaatag catactttag ttctttatct ataggacttt
    11221 gatcatctaa catcgctatc tttttatcat ccatatactt tttaaatttg tctgaaagtt
    11281 taaatttaag acctaaatat tcataaacat attctttttt tgtttttaaa gtataatctg
    11341 atggcttaaa tgtttcattt ttatccttct cattagttga catacctgcc tcttcagaac
    11401 ttatagattg tccttcttta attttattgc tttcattact ttttttacac ccaactgtac
    11461 ttaagctcaa acataacgta agtaataggc atgctcctct tgatactatt tttttcataa
    11521 atttttcctc ctttattcct cctttatttt tacacaatag cagtttataa ctactttcaa
    11581 taatattcta cacacctttt atgaaacgtt gatgaaatat aattttttat ttttccaaac
    11641 tatggtataa taatataaat attatgctta aagttgttct tacacgatta gtatatgatc
    11701 gattagtata tgagcaagca cagtaagtgt acggacactt acgaaaaaat tgatgaagca
    11761 gccctctggg atatgcttct ttttttatca atttttaact tgttagggta taccctaaga
    11821 ccccgaaata cattcaaagg aggatttacc tatggcaaat agaatacgaa atgaaagact
    11881 ggaaattaaa ctaactgaag aagaaaaggc tctttttgag gagaaacgaa agctcgcaag
    11941 gtgcagaaat atgagccact ttatccgcaa atgcgttttg gaaaaggaaa tatatcaagt
    12001 ggatttagaa ccgtttagag atttacaggg cttactgtcc aatgctacaa gcaacatcaa
    12061 tcagattgca aagcgtgtaa attccaccgg cataatctat aaggacgata taaacgatat
    12121 gaaaaagcag attgaatatt tctcaaaaga gttgtggcaa atacattctc tgcttcttaa
    12181 cagaacttct ggaggtgatt aaatcttatg gctataacaa aaatacatcc tataaaatca
    12241 acccttaatc ttgccatttc atatattgtt aatggaaaaa aaacagatga gcaaatctta
    12301 gtaagcactc ataaatgcca tcaagaaact gctcataccc agtttttaag aacacgaaat
    12361 gatgttggaa caaacggaac tgttcttgcc agacacctta ttcaatcctt tttacccgga
    12421 gaagcaagtc ctaaaatggc acatcaaatc ggtcttgaac tgtgtaaaaa gatattaaag
    12481 gacgagtacg aatttgtctt atctacccac atagataaag ggcatatcca caaccatatc
    12541 atcttcaata atgtgaatat ggtaacaggc aagtgctatc aatccaacaa gaaaagctac
    12601 cacaaaatca ggtatcaaag cgataagcta tgcaaagaaa acaacctttc tgtgattgat
    12661 gagttttacg aaagctataa gagaaagtac aaaacaaatg gcaagtcatg gtatgaaaat
    12721 gagcaatcta agagaggtac ttcttggaaa agcagacttc aatttgacat tgatcgtctt
    12781 ataaaacagt ctaagtattg ggaagaattt ctaaaaaaaa tggcggagct tggttatgaa
    12841 ataaagtatg ggaaacacat agcctttaaa ccaaaagata agacaagatt tacaagagct
    12901 aaaacgattg gtgaggacta tacagaagaa agattaaaag agcgtattac agaaaatcaa
    12961 tctattacaa ctccacctgt caaaagacga atcggaaata tcatcaatat gaatgccaat
    13021 gccaaagtga aagagagcaa aggctatgaa tattgggcaa ccaaacataa cttaaataca
    13081 atggctgagt cagttatttt tatcagagaa catggcatta actccattaa gcagcttgat
    13141 aaatacattc aaaaaagtgc tgaagaaaga caagacttac aagataaaat gaaagaaatt
    13201 gacaaggata tgcagctact ttccgataca atggagcagg ttcatactgt taaaaaatat
    13261 cgagcctact acaaagaata taaagcaaac ccttccgata aagcattttt tgaggagcat
    13321 aaggctgaaa ttacacacta tgaaatagct ctttcaaagc taaaaaaatc ctattcaaag
    13381 ctgccgaatt caaaggatat tttagataaa cttaataaac tgcaagaaaa aaagaatacc
    13441 cttattcaag agtattcttc tacaaaatct actatggacg aactttatca aatacgaaag
    13501 aactatggaa tttacatggg taaggagatg gaacgataac tctaatatta tatagctttc
    13561 ttatttttat aatattaaat tgaaatgcct atcaatgatg caagcatttt tatacctaaa
    13621 gctaaatcag gaattaaagc tacggaagta gtgataaaat gcatactttt taaacaagta
    13681 gtcaatactt ctttcttcgg atgcttctgc ttaatttcat tttttatatt atctaaattc
    13741 tgattaagta ttgatttgga gttatcatcc tccatacctt taattaagtc ttctacattg
    13801 ttaattgcct tgattacttc ttcagtatta aatttatttt cttggtcagc gttaatcgtg
    13861 cttccgtgtt ccgcaatatt gacttgaact ccagaactat taacattgat attatataga
    13921 gcattatttt catcgtaccc catatcggta gaaatatctt tcatataatt ttcaacctca
    13981 agtacaaacg gatagaccat tctatcgcca aacgcctttg ccatatcttg atatttatta
    14041 ctgccagcat aataccagcc aaaagtatat gtaggtagat tatttcctgt tataaaggaa
    14101 aatgctctat acagtagata cgtcctttta ttactatcat tgccaagatt caaaacttta
    14161 cgtccataag aattacttat cgtatttaat aagtcatcta agccatcaac atcatattct
    14221 aaactatgca aatactcttt tatcaacaat gtttgctcta tataatttat catttcttcc
    14281 aaatttgcat tctgttctcc ataatatgaa ttcataacat ttgaagctaa tatcctaaat
    14341 tttctactga tttttctaag ttctttttta ctaatctcca tctatcatct ctccacaaaa
    14401 cgggcaataa ctaccttcta atttttttat actacttatt ctagcggttt tgccacaaca
    14461 caaatatttc ttttcttcat aatctccagt ttttcttcct atcggaagtt ctgcttcctt
    14521 tttaatctct tttccacgtt taaactttat acttttacta ttcctaaaac ttttttcaag
    14581 attctttgaa aaatcattta gcatttcagc aacttgattt tcaactattc gttctgctaa
    14641 ttcataaata tcatcgtcta aataattctc gtgtgttaaa ccacaattag gacaccaaat
    14701 atctattgtg ctttcagcct caatatcttc caccgttaac ataaattttt cactacaact
    14761 tggacatttt aattgaacat atccctcttc atccgctgga acagaaattt caaattttat
    14821 atcattagcc atttaaattt catctcctca caataaaagt tataatttca ccactggtag
    14881 actacaaggt cgtgcatctt tacgtacaat aacaacacta ttctcagcac gtgtaacagc
    14941 aacatataat ctagccttac tttgttcttt tagagatgtg ttattgttaa taatccaatc
    15001 ccacatcggt tgagtcggat atataagtac tcgttggaat tccaagcctt ttgattctcc
    15061 aaaattataa tatggatatt cattattgac ggatgtcctt ttattttctc tcaactgcat
    15121 tgcattaaat ttctgcaaat acttctctac atctgcttta tcaattatca taacaccatc
    15181 tgattcatct tcagctctac gcacaaaatt tgtagatgtt ctctctggat acagactatt
    15241 cgcaaattta catataatat cattgtttct ataagtacaa ttaagtgtcg tttcatcaat
    15301 tcttacatct aattttctgc actcattttt taaaaactca tctattttcc cacaactata
    15361 ttttttatat ttttttgatg agtgagttga atatgtgcat tgtctaggat ccccgaccat
    15421 tatcacttca attcttgaag ctattaaaca ctttaatatt tccaaatcat accccaccat
    15481 atcttggact tcatcaacat atatacagtc atacattcca ctgattcttt caaaaacttt
    15541 tccattacat ttatcattta actctacaac acactctgat aatttatcag tatataccct
    15601 gttatctttg gcaaaatatt tgttggatgt tttctttacg tacgtattgt tctgtccatt
    15661 tacaaatata attccttcaa ttcgttcctt aaatctactt ccttgataag gtctaatacc
    15721 atcttttaac aaaaaagaaa accatgtaga aactgtaata ttattaggaa ttacaccaac
    15781 tttattctta attcggctaa ttatttgttg ctcatttgta atagtatatg ttaggattag
    15841 tattttttta tgagtattaa gagaatcctc tataattttt cttgtttttc cagatccagc
    15901 agcagctata tataatttcc cactcatatt agaataccct cctttatata ttgtggaaat
    15961 tctatagctt tatcactttc aaaaatcttt aaagcaactt ctgttttatg ccccttcata
    16021 tatttaagta aatcatctgg atcttcatat gatttattca atatctcgtt taaaatagac
    16081 atggaattcg atctatatat acacggttcc aaagtattat agttgtaatt ttccaaagaa
    16141 ccattatatt catgggtact atcatcaaaa tatatattta tattctcaca acaattatct
    16201 cctaaataat cgctatattt tctatttagt gcatcaatat ttccatcatt atcggttatc
    16261 actgacacac gtaattctag caattttgcg atttccaaaa atcttaaaaa agaaattcca
    16321 accgatatta tatctattcc atcattaata ggcaatttac cattatgaga gtccatatat
    16381 gctctttgaa caattagttc gtccgaatca ccttcgacta aaattgcttt atttgataat
    16441 aataatcgta acgtatcata acctgcaatt cttttaaaaa ataaatttgt ttcatttgat
    16501 aaatcaccta gccgtagtgt ttttcctcga tttagtaaaa ttaaattttc aagtcctaat
    16561 ttatttgata caaaactact atgcgtagta actattatct gtttttcttt tactgactca
    16621 tttactttat ctaggagaat atttaagttt gaatatgaca aatggcattc aggttcttct
    16681 aaaagcacaa tctgcttatt ttctatttta tgatcactta atgccaattg tgttttaaca
    16741 atacattgtg tacctttacc aatgttatta tatggtattt catttaattg ggttaccaag
    16801 ctgctttccc atgaagtttt cgtaccgaaa tctacagaaa ttgtcaattc gttatttgat
    16861 gtttccgtta attttctatt tatattagca atattctcat ctctagaaaa ttgttctttc
    16921 ataagcctat gcgcttgcga tatattaata atttcattat catctaacag attctttact
    16981 attctcgata tgtatatatc tgacccactg gtaggagtat agcttgtcgt atcaatcatt
    17041 gcagacttaa ttggaatgct ccttgcagtt atattttctc tggcaaaaga catccaactt
    17101 acctcataat attcaattgg caacgatgtt atcttgttct tacaaagcat tttatactca
    17161 ttgctatatt tttcatcaaa actaattgat aaacaaatcc caggggtgtc tttagttttt
    17221 tcagaatttc catcacctaa aaaacttggc atttctgcat caaaatacgc ttcaataatt
    17281 attttaggag gttctacatt cactccttcg ttgacttctc taatatagtt ttccactata
    17341 ttcttattga aaaggtactg agtaagttca ttcctaatat ttttcccatg ataagttcca
    17401 gttaaaacca gattaatagc ttccaaaatt gtagatttac ctgttccatt attgccaact
    17461 aatatattaa tcccattttc aaaatcaact ataaaccaat ctgaaaagca tttaaaattc
    17521 tttattttta atttcctaat tgacatttta tcccccttct ttatctaata taaaaatcat
    17581 gattttagaa tataaatatt gataactata taaatacagt tatgagtttc ctgtttaatc
    17641 acaatcataa attttttatg ctacatccct taatattttt tcaagcaata atttaacaat
    17701 aagttttatg atcctctagc tcctgaaaat aaatctatga ctgtaaactt agactcccat
    17761 tctccttaca tcttcaataa ttacgcactc cataatatct tcaatatcac aatccaaagc
    17821 attacaaatt ctaagcaaaa catccgtagt tacattctgt ccattcttca ttttgtaaaa
    17881 tgtactttta ctcacttttg ccttttccat caattcatta tttgtcatat ctaaatcaat
    17941 gagttttttg aacaatcttt tataactaaa aaccctcatt cttatcctcc gttcaagaaa
    18001 actatatcta gtatattata ccacaaaata tacttttttc ataaatatat tctctttaat
    18061 cgaacttgca taaataaaaa agagccggtg cagtcctaag accacaccga ccgtaaattt
    18121 atctctcagc ttctttgctt gcttctttct tttctttcgg tttttcctta tcttcagcct
    18181 gatatttttt gatagctcca agtatagatt ctttcttttc ttcctgtcct ttcatctgtt
    18241 cctttgcctt taacagtttt gaagaaagta tcctgatatt gctatgctcc ttgccgttat
    18301 catcaatgga agttctgatc tgcccaaaga gtttgacaaa atctccctgc ttaaagtcct
    18361 ttggaatatc actcttttct ccataaacgg agcagttgtg atataactta tttccctcat
    18421 catctttaga tactacggag aagtttgcta ccttaaaggt ttctccgttc ttattttccc
    18481 tttcaattac atcaacttct ccaacgatat ttccgacaat attgataaga ttatcctttt
    18541 cttcccgaac ataaagcagg tcttttatct gcccctgtcc ccttaaatcc tcaatcatat
    18601 agtcaaactc ctcatgcagc aaattaaggc tatcattgtc catataagcg tcatatagct
    18661 tatctaaagc actttcatca ttgataccct tttccatgct tataatcgct ttaacaaagt
    18721 ctttatgatt atcacttaac atatcttcaa ctctatctct cattgttttg taatccatgt
    18781 ttttatctcc tttcatggta aaagggaggt tgccctccct ttatcttaaa aattcctgct
    18841 cttttatttt ttgtttttct tctgttttac tttcttcctg ataggctctt atctgtccta
    18901 agatagacgg cttatcatct gatttttgtg tggtttcttc tttgctatga agattgtcct
    18961 ccacatcata agttgactca aaataatcac tgtccttaaa atcactatca tatctatccg
    19021 gaataccgtc attatcaagg tcttttgcaa acggatcata gaagttaccc tcatcgtcaa
    19081 tatcaagtcc cgttgctact cttaaatctt cagaatcaac ataaaccaaa tcactaaatg
    19141 tagataactc aatttcattt ttcatatttc ttagagcttc attttctccc ttattttcat
    19201 aatcaaagct ctctgtttta ataggcgtat catcaatgta ctgtgtccaa gttttctcct
    19261 ctaaattaag ctcatattga ataccatgtc tttcatccgg tgtattggta taggcgatac
    19321 cgatatgctt taagtcagga tatagagtat gaaactcatc ataactgtga ttttcttcat
    19381 actctctgtt acagaagtca atgattgctc tttttacatc ttccacaaga ggattatcat
    19441 ttttcttttc ttccacctct cccatatcga gcagcttatt taactctgca agtcttaata
    19501 ccttagtttt caactcatct gccttttcaa aaggcttttt taattcctct ttggcatttt
    19561 caagctgttc ttttgtactt atgagctttt cttcaagcat atttagtttc tccggcattt
    19621 tctcaagggc attatccagt cttgtgatat taccgtccgc acttgttcca agctctccta
    19681 aatgctttgc aacaccgttt aagctgaaat tatgagaatt agtaaagaag ttatagctta
    19741 cctctaaatc catatttcta tactttccta taaccttgct ttcattgatt tttacctttg
    19801 aaatagcttc aagtaatctt tctccggcta atttcttatc ggtaattttt tctcccgcaa
    19861 tggtaataga agtaaacttt tcttcacctt ccgctttagg ctctacattg gatatatctt
    19921 ttttgacagc ttctatgagc ttttctgttc ttgcgatttc ttcaggatag ttttttgcca
    19981 ccttatcctc taatctgtaa cggttagact tatagtttgc ttcaagcatt ttaagttttg
    20041 taacctcatt atccaaatcc atcttttctt taatctttgg atcacccgtt gccaacgctt
    20101 taatttcggc gtaatttaag ctgctttcgt ccacatcttc tgccacacga acaggagtct
    20161 tactggtcat aatctgtgaa atgaactttt gcttattctc tatcgtctgc caaaggtagg
    20221 catcaaaggt attctctgta acataacgat agatatttac cttctcattt tcatttccct
    20281 gccttacaat tctacccgca cgctgttcaa gatcagctgg tctccaaggc acatccaaat
    20341 catgaagtgc aatcagttta ttttgcacat tcgttccggc tcccatcttc tgcgttgaac
    20401 ccattaagat acgaacttcg ccttttctta cctttgcaaa gagttcatcc ttttgcttat
    20461 cagaattcgc ttcatggata aaggcgattt cttctttcgg tattcccatt gctacaagtt
    20521 tttctctaat atcatcatag atattaaatt ctccatctcc ttttggagta gacatatcgg
    20581 agaaaagtag ctgtgttgac ctattttctt ttgtcttatc ccaaatagca aatacatttt
    20641 ttacgcatac atttacttta ctatcagggt tatcaggaag taggggattg attaaacgct
    20701 gatctaaggc aagtttctta ccgtcattgg taatcttaag catattatca acatccggct
    20761 ctacaaccct atttctgaca tcatccgctc tttcagaaag gctctttagg atttcctttt
    20821 gttcttcaga aggctcagtt ttaataactt cataattagc ttcaggtgta ggaaggttaa
    20881 gcatatccgc agtctgaata tctgctactt ccttgaacat actcattagt tcaggcaagt
    20941 tatagaactt tgaaaatctc gtttttaccc tatatcctgt tccttcagga gatagttcaa
    21001 aggagctttg tgtttctcca aaagtggaag cccaagaatc aaaatgctct aagtgatttt
    21061 tctttaaact ctcatactga agataccttt gcattgtata aagctctgtc attgagttac
    21121 ttacaggtgt tcctgtggca aaaactatgc cttttcctcc tgtcatttca tccatatatc
    21181 tgcatttcat aaacatatcg gaggacttaa aggcttctga ctgcccgata cctgcaacat
    21241 ttctcatttt ggtatagaga taaaggttct tgtaattatg tgcctcgtca acaaagagca
    21301 tatcaactcc cagttcctca aaggtaatta catcgtcttt cttaaaatca tcatttaatt
    21361 tctcaagcct tgtttccagt tttttctttg tcttttcaag ctgttttact gtaaagttct
    21421 gatttctgtc atgcttgtat tcttctacat agtttacgat ttcattaatc tgatcttgga
    21481 tgtgcttttc ctgatattcc ttactcatcg ggattttttc aaactgcgta tgcccgataa
    21541 caaccgcatc atattcccct gtggcaatct ttccgataaa tctctttctg ttcttcggtt
    21601 caaagtcttt tttatcagcc accataatat tagccgacgg atatagctgc ataaattcac
    21661 gaccgatttg tcctgttaag tgatttggaa caacaaacaa agacttactg cacattccaa
    21721 gtcttttact ttccattgcg gaagctacca tctcaaaggt ctttccactg cctaccacat
    21781 gagcaagcaa tgtgtttcct ccataaagac ttcttgctat ggcatttctt tgatgaggtc
    21841 ttaaatcaat ttctgtattc attccctcaa aagaaagatt gcttccatca tattctctgt
    21901 ttcggattga gttaaaacgc tcattataca gttttacaag acggtttctt ctttcctgat
    21961 cgttaaatat ccagttctta aattcttctt ttaatagctc ctgcttttgt cctgcaagca
    22021 tggtttcttt tttatttaag acagaagttt tagaaccgtc cggatttata atctggtcaa
    22081 atacctttgt ttctttaaga tttagggcat cttcaatcag cttataggca tttacccttg
    22141 atgtaccaaa ggtcatttct gcaaggtcat ttcctttatc cctgcttttt ccctctacat
    22201 tccattcact tgtaatattt gaaaatttaa ccttaatatc ccatcttgca tatcccggag
    22261 tttttaaagt ttcaaagatg aatttttcaa tatctttaat cggtatccaa gtcgcaccaa
    22321 gacgcacatt gatttcactt gcttctaatt cctttggaag aactttggta agctctgctt
    22381 tttgatattc caaacgattc atttcatagc ttatcagctc tttttctttt ccattctcaa
    22441 caaagccaag atgaggaagc tctcgttccg tttgtcttaa ctttgcaagg tagctgtcta
    22501 caatggcaat cttatctcta atatttccgc ttaggtactc atcctttgtt acatagccgt
    22561 acttatatga attactgccg tttgcacaag cgaaaggcaa atctccatct tcaaggttaa
    22621 aggataatgg tctataaaag ttttgttctt ctctgatgtt tagataaatt tctcctctaa
    22681 gttcttctat caaagttggt ctgtctttac ctgtaaggct ttccatatac tcaaaatcca
    22741 catagccttt ttcagatacc gataagacaa gagcttcaag ggaagtgtcc acatggtcta
    22801 tgactttggc ttttgtaatg gttctctttg agaaaatatc cccctttgcc ttgacgtttt
    22861 cttcttcatc aagaatttca atggaagata caagagggaa attgctatcc tcttttaagg
    22921 ctctggtatt gcttaaatta tttataaagc catgcttctt tgaaaagttg tcatagactt
    22981 cattaagctt ttcttgagct ttctttacct catcatcgga atagtcctct ttctgcttgt
    23041 agataacatc tttaagggca gtattcagtt caagatagtc cttaatcttt tccttattct
    23101 tatccgttat ctctttcttg atgaaaaggg agttttctct gtaatagact tcatcatcaa
    23161 taacggtata ggaaaagttt ttgacatcat ccgttgcagg aattgaagtg atttcatcat
    23221 ctaaaagctc tatttcttca taagttgatc ctttggatat ttcttcactt gcttttgtca
    23281 gaagctcttt taagtcggca ttttctttcg gcaggcacgc aagtgtgttt ccaaatcttc
    23341 ctgaaacttc acgcattgtg ccgagtacct gctcaggatg atctacaaaa tatttgttat
    23401 aggtaagacc attttcatct tcagcaaggt gaatccagtc ctcatctctt tctctgatac
    23461 tgtccctttt ctttaggaat ataatatctg aggttacttc tgttccggct atgcccttaa
    23521 aggtatcatt tggaagtctg attgccccta aaaactctgc tcttgctgca agatagcgtc
    23581 ttacactttc atccttttta tccattgttc cacttgatgt gataaaggcg ataataccgc
    23641 cgtttctgac tttatcaatg gatttcgcaa agaaataatc atggataagg aagttatttt
    23701 tgttatactc cctgtcattt accttatatt ctccaaaggg tatattgccg ataaccgcat
    23761 caaagaagtt attggaaaag gaggtttctt ccagtccttt aatctgcact tcactttcag
    23821 ggtatagtag ttttccaatt cgaccgctta ccgagtcaag ttctatgcca taaaacttgg
    23881 acttattcat ttcatcaggg atattgccta taaagtttcc gatgcccatt gatggttcta
    23941 aaatgtttcc ctgtttaaat cccatatccg aaagcgtctt atatattccg tcaattacgg
    24001 ttttcggtgt ataaaagctc gttaaagtag attctcttgc agcttcgtat tctgcatgtg
    24061 ataagttctc atttagaaaa gcccttgctt ctttccactg accttccttg ctttcatcaa
    24121 agacttcgga aagtccgccc cagcctacat accttgctaa aacttcctga gctgtactgt
    24181 caagctctct ttgtccgctc tccactctgt taagcatgga aattgcttca aggttattat
    24241 ttaacctttc gcttggactt aacttttcag ggagcgtttc ttctgtaatt ttaaagttat
    24301 gagcttccgc tttccttatt tgagtttcct cagtagtctg ctctggctgt ttatatgtca
    24361 ggttttcaaa taccctctca aggtcgcttt ccagacgata aggaatgaca tcgaagcttg
    24421 ttatcatccc gccaaaatac tcggtgttgt ccttcaccgt taccgtattt aggttctttc
    24481 tcatatcatc aaagcgtgtt atggtatggt ttttgtcctt gtatttaact tcatcaccga
    24541 caataaagtt cggtctttca agacttattt tattcagcaa gtcctcatta tctgtaaagt
    24601 ctacaatcgg aatttgatgg tttccacttc ttaccggatc aagccacaaa tcatatcttc
    24661 ctgtgatttc atttttagaa atttctctga ctttatattc ttcatgatta aaatagacgg
    24721 tatcgcctgc tttaactgca aactcttcta aaaggctgtc ctctttttca ttcagttcta
    24781 cttcttctct ttctttgagg taatcaaata aggtagcttg aactgtgcca cgctttgtag
    24841 cttcttctgt ttcttcaggt gcttcaagct ctactttatc ttcaaagcta agttctccat
    24901 taaagacaaa ctgaagctcc tgctcatcca tttgttttat aagctctata tctttaagtt
    24961 ctctaaaggt ctttggaaaa tcctctaaaa tgagcctctg ggcatttaat tctttcagtt
    25021 cctcaaggtt aaaatatccc cattcaggct ctattccaag aacaagtcca aaggcatcac
    25081 cgctttccct gtcatactcc gtcatatacc aagtccaatt tgaacgaaaa ggaataatat
    25141 atgctgcatg aacctgctta tccgctaaat ctacatcctc ctgtgcgtaa atctctggca
    25201 ctctttcaag aatttcatca ggcattagat ttccaggatc atttttagaa taatattgcg
    25261 gttcttttac ttcttctatc tctgccgtac tttctgtttc cttttcctca aaataaagtt
    25321 ttttagaaag cagctcatca atatgctttg caacacttgt ccaagtaagg aacacatcat
    25381 tacaatcatt cttctgtaat ttaaaaccct ttgcatcgtg ccattcatca cttcccattg
    25441 cacctgacac aacgtgagaa tgtcctccga ttccatattc atcttttagg aaattggctt
    25501 tttcctgcaa agtatgattt tctttaaaaa acttggtgat tcgttctttt cctctatcaa
    25561 cgccgcttcc tcttgaaagg ctttcaagga cttcatcttc tgtaataaag agctttacct
    25621 tcggaagttc tgtcagattg gtactatatt cctttcgttg cagctcaagt tcttgcaact
    25681 tctgataaag actatctacc ttgtgataat gataccttaa tacatttctg ctttctttgt
    25741 atccttctaa aaacctgcta tattctttga ttgtttcttt cagatattca ggatttttta
    25801 atgcttcaga taatcttttc gtttcttgcg gaaagccacc acctctttca aaagagttga
    25861 aatatccttg ctctattcct tcttcgctta aatcgtgcgt tagataccat aaggattctg
    25921 aaattctatc cctttcatag tcctgtgcct ctaaaagttc tacatttgta gcaaactcac
    25981 ctctgtcaag aagttcattt atcctacttg ctgcatcact ccaacttaaa atttgtgtat
    26041 tatcctctct tgccgatgtt ccataagcta aatgaatacc tttatccgaa taccaggaag
    26101 atacttctct ttcatcaatg taaaatccgt ttccgccttt aaaggtatct ttgaggtatt
    26161 ctcccaattc ttctatgctc ttgccttttg aaaactcagc aataacagga agcctaccgc
    26221 catcatgatt tcctccgtta ataaggacgg tatctatatc cttctcagtt agcggaatag
    26281 taaggcttat ctgtccataa gaattttcgg gtaaagaaaa agaagccttt tcagcttcta
    26341 ttatcccttt atccgtattg tcttttaaat ctccactact tccttgatgg tcatttcttt
    26401 gagagctgaa atcatcgccc catactgcgg attgttctcg tcctctatct tccaagctgc
    26461 tatcaacttt ggcttctctg ctctcataaa ctctactgcc tgtttctgaa tatccatcag
    26521 gtgtcctgtc agcttcttct ccttgtatag atccaaaagc atctcgaagt ggctctgctc
    26581 ctcgctctgt gaaaggtaat tcagcctcat cactgcgtag gtcggattcg gatacttcct
    26641 tataaagtcc gtctgttcct ccaagctgtt tagcgtattc tccctgattt tttctattat
    26701 ctcgtccgta ctctccattt cggagaactc gctcgttttc atttctttcc tgatcatctc
    26761 gtcgaaatac attttcttct acctcctcta attcttcttt aattttatta tatcccgctt
    26821 cttttcctat taaaacttct ttttgaagct caagctcttt gcttttttga atggtttcat
    26881 caataatctt tccgctaata tccgatacac tttcaccaag gctcattaag gatatgctgt
    26941 caaatctttg aaaattttct cttaaaagct cataatccat cggataatct aacttaaatc
    27001 ttgaagctac cgcatagctt accgagtccc ttacaaactt ggtaaatgat attctatcct
    27061 catccgctac tctaagttca ttcattaagg tatctatttt ttcatcaccg tagagtctgc
    27121 ttaaagaaaa gatgttttca agtgtgcttt cactttcctc atagccctcg ctttttatca
    27181 tttcctttaa gacatcttga tgggcttctt tatcaaatct ccaaagattg acctcgttga
    27241 catccctgtt ttttgaaact gtctggctta tatcaaagat atagtccacc tttttatatg
    27301 tgccgtaatc ctctaaaatt ggtatccctt tctgccctcg cataaccgtt cggttaaagc
    27361 gttccctcca gtagtcgaac ttggcacagg ctgtcgcttc aggattttta tcatatatac
    27421 ttaattgact tctaaaatca tacctttgat tgtttccgac aacttttagg agctttagat
    27481 attctgcttc attatacaaa acatcttgtt ttattagctc caaaatgtta tgaaaatcat
    27541 ttattcgcat ttttacctcc ttcttttttc aaacaaaaaa ggcggttaga ttttactcta
    27601 atcaccttcg ctatatacca aatattatta ctctatttca attttttaat tgattcactt
    27661 tcagatagta tcttcatttg ttcaatagct attgcattta atttttccaa tctttcagat
    27721 tggctcatgc cttcatttat aaataccgca ttaagattct caagattaga aaggcagaca
    27781 agctgtgata tatccgcata atctctaata ttgcctttta aatccggatt ttcatcacgc
    27841 cactccttag ctgttattcc aaatagagaa acattaagaa tatccgcttc acttgcataa
    27901 ataaaattga ttttttcttt tgaaagttta tccggtacaa gtttgttttt aatggcatct
    27961 gtgtgaattc tataattgat ttttgccaaa tttctcttaa tatcccatcc taacttcttt
    28021 tgttcttctt cctttaggcg ttcaaattcc ttgattaaat ataatttaaa gtatggcgat
    28081 acccaagaag caaactcaaa cgctatatct ttatgagcat aagttccacc atatcttcct
    28141 gctctcgcaa cgataccgat agaattagtt ttttccaccc attgcttgac cgataatata
    28201 aaacggttaa atcctgcttc atttttaatt ccctcgaatt cgggggaatt aaaattcgtg
    28261 ttatacattt cttcccaaat acctaaaaac tctattgtat tcttgtttct gagccacttt
    28321 tcaatcaaag cagaaccgtt ctcaatattt ctaaccatat ccgttaaaga aatataatct
    28381 ctattgtcta tggcaataat gctgatttct gaaccttcaa catttatttt agacatataa
    28441 tcacctctca atcattaaat tgattacttt gcattatagc atttttagag cattttttcc
    28501 acgctgaaat tagtgctgtc ttacttgcga tatgtcaaaa ccgcttcttt tatctcattt
    28561 tcaatcgcat ctaaaaattc tttcttagag gatttaccct gagcaatatc agataattcc
    28621 atttcccact ttgctgtcgt ttctgccgac ttaaaggtat ctgctacaat cgttacaagg
    28681 ctgattcctt tatgcgttgc aattagattc ttcttatctc tttcgacaaa tctcttaaag
    28741 attagatttt caataattcc tgcccttgtt gccggagtgc caagaccttt tctttccacc
    28801 tctacacctt tttctaaggc atcatttccc gcaatctcca tagactttaa gagcgtatct
    28861 tcagtaaagt gtttcggagg ttgggtaaat ttttctttaa cctctttatt ttcaacgctc
    28921 aaaacatcac caacacttac atcgggcaat acagcatctt cactcttttt tgatttgtat
    28981 tctttaaggt atttgctaaa gccttcatcc ttaattacct tacctgaact tgtaaattca
    29041 aaaccgtcaa atgaagctac aatctttgtt gtattttcaa taagcggata gcctacactt
    29101 gcatgaaatt tatcggatat aaggaagtaa acttttgctt cacttaaagg taattcagaa
    29161 acatcttctt ttactgaact gattgtcggt atgattgcat gatggtccgt aaccttttta
    29221 gagttaaata ctaccttgat acgctctgta tcaaagtcat ttttacccaa aatgttgttg
    29281 actgtactca caatcatatc ctctgttaaa catctgctgt ctgttctggg gtaagtaatc
    29341 agtttctttt catacaggct ttgagtatag tcaagtgtct gcttggcact atatccaaaa
    29401 tatttattgc actccctttg aagcgtagtt aggtcaaaag gtaaatcagg ctttgtaatt
    29461 ttttcttttt gaataacatc agtaatttca atcttatcgc ctactaaact tataagctgt
    29521 tcagcagcaa cttcatcatc aattctgtct gtggagagtg taaatccatt catagaaagc
    29581 tctactgtgt aatatttttc tttctgataa tttgctatct catcatctct ttttacaatc
    29641 atggcaaggg taggtgtctg cactctgcca acactgtaat tttgctgata cagacaagag
    29701 tagagcctac taatattcat tccgactaac caatccgcaa tagctcttgc ctgtgccgat
    29761 tcaaagagat tatcatagtc ttttccgtct gttaggttat caaaaccctc tttaatggca
    29821 ctatcttcca ttgaagaaat ccaaaggcgt ttcatcttct ttttacaatt cacttgatta
    29881 tatacaagtc taaaaatact ttctccctca cgccctgcat cgcacgcatt gataaccgta
    29941 tcaacctcct tatcattcat cagcttttta aggatgttaa actgcttctt ggttgccttt
    30001 gctacttcat acttgtagtc gctcggaata atcggtaaat cagccatatt ccacttggcg
    30061 tacttttcat cataagcatc cggatttgcc atttgaatta ggtgtccaac gcaccagctc
    30121 accctatatc cgtttccttc ataatatccg tctttctttt tatttgctcc aataaccttt
    30181 gcaatggaga ttgcaacact cggcttttct gcaataacta acttcatctg tgttttcctc
    30241 ctgttataaa tcaaaatagg gcgaaagatg ttactctccc gccctaactt tgcacccaaa
    30301 ataatatgtt tttaggtgcg tttattcttc atcttctact tcatctgcat catttatttc
    30361 ttcctcgctt tcagattcag aaaagaaatc atcatcttcc tcctctaagg cttcaagttc
    30421 tttatcttct ttctttttca caaccttaaa atagtagcct gctcctaatg caccacctac
    30481 tacaagaagc aaaataatat aggttcctaa attactcttt tcgtccttct tctcaggctt
    30541 tacttcttcc ttaacaggtt cttcttttac tacttcctgc tttggtgctt cttttttctc
    30601 caccatgtta agcaaatcat cttcagatac ttccgtaaga agcattacat tctcactatc
    30661 ttcatcatga ttgatgatta aatgaaaagt cttaccgttt ttcgtttgaa aggtaataaa
    30721 ctgccttgca tctgccgaat actgatccgt ttctttgtta tcgctgctat ctccatgatg
    30781 aatcggatac tccttatttg cattatcttt attctccgta acggatgctc tcgcctttga
    30841 cggagatgaa gctactccct tgttggtgtt tacactctta cttgcagacc ccattgatga
    30901 atcctgactg ttattcgcag gagcttttgc cgtcaactta ttgggatagc gaacttcctt
    30961 ttctttctcc tttacttccg ttttgccttt accggtatcc tttgtagtat tacctgagct
    31021 taccgaatcc gatgagcctt taccggtact acttacagaa gtctgtggtg tttgaggaga
    31081 aatacctgaa cttgtcttaa ttccggaaat aggactaatc ggtgtaactg attgtgatgt
    31141 tacaggagct ttatttgttt cagttgcctt tttctcaagc tctttcacct tttccgtcag
    31201 cttatctatt tcctttttca tatcttctga taagtcctta tttcccttat ctttcttcat
    31261 tttttctttc aggctatcaa tttcatcttc aaggtcttta attttattct tttgcttatc
    31321 acttaatttg tctttgtcct taagttcgca atttaactta tcaagtttat cctgaagctc
    31381 ttttgcctct ttttctattt ttgaaatatc gtctttagaa agctctgtct gcgttccctt
    31441 atcttctgtc tgagtgcatt catcctgctt tggcttttct tcttccgtct gcgtttcctt
    31501 atccttaacc tccatctttg ctaccttacg gataatctca tcttttccat ctccctttac
    31561 ctcataaaac agaaattcat caatgaattt catatcatct gggagcattg gaagatctcc
    31621 gctatcaata agttgtcctt tctcagcctt tatcttttct tccttaaaga ccttttcatc
    31681 ttcaaagata taccttacat ttacttcaac ttccgtttga acagcttcgt tttttgttgg
    31741 agcttcagat ttttgctcct gtgcataaac aaccgtccaa agacctaaaa tacaactaat
    31801 acttacaatt accgcaagtg ctaccgtcca aaacttatta ttctttttca aactggtttt
    31861 catttgtttt ctcctttttc atttctgctt tttgtctgtt tacctttgct atcaaatcgc
    31921 tgattgtgat gttgttcttt ctgcaaatgg caatgatttc ttcattttca agctcttctt
    31981 cacgaatgaa taaaggctcc aattcttcat caatcagagc ctttttatcc tttaacttct
    32041 ttattctgtt ttttactgtt gtaagttcct ttttcaaatt gttacctcct atttctttac
    32101 atttggcgga aaaccgaatc ctacgggatg gtgcttacaa aatgttgcta tccaatcatc
    32161 taaggtagtt actcttattc gaccgatgtt atcaatcact tttccatctc caatgtaaat
    32221 acctacatga ccataggtaa gccctgcact gcttccgcta ctgctgcttt caactgccac
    32281 aagcattccc acttttagct ttgacctgtc tgatgtaaag gtatagtttc ggtacatatc
    32341 acaggcattc ccgcctatat atccaagtcc tgcattttga tagacctgtg atacccacat
    32401 ggcacaccag cctgcacccg gagatggcgt aatgtatgca gcattaacaa tcttcttttg
    32461 tacttccgta gatgcctcat actcttttcc accgccgata cctccatttg cactgataag
    32521 gtcagaatta ccaaaggctt ctctcatatt gccttgtgca aggaacaagg cttcatagtg
    32581 ctttagatta tcgggatagt ttgcaaagac ctttcggata atactgtcca tctctttttt
    32641 atgaagcgtt acgatgagtt ttttatactc ataaggttct tcgtggcttt cggtatattc
    32701 attgccatct tcatcggtat aggtttccgt aaccgtcctg tatctgattt caatttcttc
    32761 cctatactca aggtcataca tggactcaaa taattctttt aatatggatt ctacttccga
    32821 tacactcttt acctctccgc accttgatgt aatgtaggat aaaagctcat ggacattatg
    32881 accgatgtat tctgtattat ttaagatata ttcatcatat ccaggataat tttctttcac
    32941 atagtcaact tcactttgaa gctcaccctc catatctgaa aatttctgat tgatttcact
    33001 taggacattt ggctttgata aatagcttgt tgtaagcaca gagcttgtag agttcataaa
    33061 gcctgtcatt cccgttcctg caaagttaat cacaaaagtc cctaagataa tgatacctat
    33121 gaaaataagc attagtcctt ttgcttttcg gataatcata tcctttgagc ttttcagtgt
    33181 tccgataagt ccctctttaa ttcgatctct aagccttgac ttattctcac gagaaattgc
    33241 tgcctttacc tgatttttct tttgaaatct tttatatgca cttgctcttt tatactcatc
    33301 cgtctttttc agttcctttt tggcatcacg aaattccaac tttgacttac gctttctgat
    33361 tttatagtcc ttatttgtaa ggtcatagcc tcttttagct cttttcttat cagagtactt
    33421 ctttatgcca tgaatgagct tggagcttgt atctgctgtc ttttctcctg cttccactcc
    33481 ttgattttca tcacttccat gagacaagta atctcttaca gtttcacttc ctttagcaag
    33541 tcctgaaagg gcagatacct ttaccatgtc tttttttagc tttttctttt ggtcttttga
    33601 taggctgcta taaacttttc cgctgacagc atcttttccc gcctttttat catcagcctt
    33661 atttcccttt ggtgcatcgt cctttttcct tgtataaagg ctttcagaat agttcttcct
    33721 cttgtacctg cttttttgag tcttatgatt tttaaaggaa ttttctaatg atttttcttt
    33781 atgggaaaga ttatcctcca catcataggt cgattcaaaa tagttgctgt ctctaaagtc
    33841 attatcgtat ctgtctataa tcccgtcatt atcaaggtct tttcctaaag gatcatagat
    33901 ttttccatcc ttaacctccg taatataatc tgatcttccc gtttcaattg cggaatttac
    33961 gtctccctta tctgaagttc tatatttttc gtttctcttg gatgttccat aagacttttc
    34021 attatcagca cttaccttac ttgttttttc atgcacttta tcctgaaacc gttctttctc
    34081 atgaacgatt ttacctctgt aatcatcgtt atgttttagc ttactttctt caggtatcgt
    34141 atctgtttta ctatgaaaca tctctctttc aaggcttgcc ttatgccttt ccttaaaatc
    34201 ctttttcagc ttctttccca taggctacct cacttcttcc ggcttggtag tcatcttctg
    34261 atataaaatt gtatctttcg gaaacttatc aatgaaaggc acaatggtat ttccaaagaa
    34321 taagagtccc tcaccttcat ttgaattagt aacatatctg agctgtggaa gtgagatttt
    34381 aagttttctt gcaagtatct ccctatctcc tgatgcttga ttaagcatta agacaaagtc
    34441 tgtattgtca aagatatttt ctatctcctt actcataagt aggtctttga cattctgcgt
    34501 aatacctgtt ggaatacctc cccatttacg aaatcttttc caaatctcta cggaatagga
    34561 tgctgtctgc tcatctttta aaagcaaatg gaactcgtcg atatagtacc ttgtagcctt
    34621 gtttcctctg ttttgtgaca ccttattcca cacctgatcc tgaataacaa gcataccgat
    34681 tttctttaat tgacttccaa gctccttaat atcaaaacaa aggagcttct tatttaaatc
    34741 cacatttgac ctgtgattaa agacattgag agaccccgat acatagattt ccatctctgt
    34801 tgccagcttc ttaccgacct tttcttcctg cccttttaac atatcgtata ggtcttgaag
    34861 tatcggcata ttgtcaggag taggatgttc aaaatacttt tcatagattt taggaaggca
    34921 cctatctata acggactttt cttctgctgt aagaccacta ccgcctacta caagctcaag
    34981 catactcatg ataaagtttg ccttgtcttt cagcggtgca tccccatcac cgtagttcat
    35041 gtttatatca agaggattca ggtaatcctt cgacttactg ctgaccttaa tgacttctcc
    35101 cttaaactgc cttacgaggt ttccatactc tccttccgga tcgcagataa tcacatcatc
    35161 atctgttact aaaatcgcat ttgccatttc tcttttggca ctaaaggact taccgctgcc
    35221 cggagttccg aggattagac cgtttgggtt ctttaatttc tttctatctg ccataatcag
    35281 gttatggctt agggcattta gtccgtagta aagactatta cttgaattga taaaaagctc
    35341 ctctgtggta aatggcataa atactgccgt tgatgagctt gtcaatcctc tatctatttc
    35401 aatcttattt acaccaagag gcagcacgct cactaaacct tgttcctgag aatggtcaag
    35461 tcttttcagc ttacagttat gcttatttgc aatggagctt atttgagcaa ttgtgttatc
    35521 gagcttttgc actgtccttg caaagttcat aaagacgata cttaccacaa acattcgctc
    35581 atctcttgtc tgcaagtctt ttaagaggct ctttacatcc tcaccatagg taattaagtc
    35641 cgatggaaga atgtccatat cataaccgct tcttaccgcc ttcttatttt cctcaatttt
    35701 catcttatca atatcagtgt tttttcgttt taccatctta atggcttccg attggtcgat
    35761 tgccctaata tgaaaggaaa tattgatgtt atcgtcaata tccaaaaact cggataacat
    35821 cctatctgaa agctcactgg caaggatttg aaaatgactt gttgctccga tgaatttgcc
    35881 gaacttaaag tatctactcg gtgtaaagtt aaactcatca ggcacaatat atgtctttgt
    35941 gctttccctt ttctttaggt ctttgtagga aaatttaaat gttttatttg gatttaaaac
    36001 atcatgaagt atctttagcc tttcttctcc gtttaggctt tctgctctta ctcccatact
    36061 tttaagactc gataatatat ctatctccag tctttcaagt tttgatgttg cctgttctaa
    36121 attatccgct tccactgtaa aggttacata ctttgatttt ttcaatccgt tatttccctt
    36181 tacaatctgg cttttaagca tctctctaaa ttcaaagcgt atatcgtcga aaccgtcctt
    36241 tttatccggt atctgaattg ccgacttcat ttcttcgttt cgtccgagct gattgatata
    36301 tgaaaactca atgctgatac ttggatcaaa agagtttagg aagtttgcaa attgattaaa
    36361 aatcaagtcc ctatcttcat ctaaggcaag ctgatagtta atgtcctgaa aggctatact
    36421 tttactgaag tgcttttcat caagctggca tattccgctt tttaaaagcc ttagataggg
    36481 aatggtatct tctaccgtat atcttttcgg ttctttctta aagataaggt caaggattcc
    36541 tcccttatct ttttttgact tgcaattact tttctttaag tcccgctttt gacttctcaa
    36601 tgccttttcg ttttgcatga gttttatttg ctgaattttt cttttcttgt tcaaggtaaa
    36661 cctcctttct cactctcttt tgcggttgat aaaacttatg aagataaatg tacttaaagt
    36721 atttctcaaa tgtcagtccg tctttttcaa atagtgtgat gaaaaagata ggaagcgtgg
    36781 atacgataag gaatatgaca gctatgtcat ttggcacaaa cttcctcata aataaataga
    36841 ctggtattcc aaccagtcct gcaagtgtga agcctatgag ctgccttttc gttaggttaa
    36901 aagcaacctt tgtctttacc ctatttaagt ctttagggat tggtacatat gccatatctt
    36961 acctcccatc ttctttaccc tttgacagct cctcaaagtg ttcatataca gagccgattt
    37021 cttcctgaat agtcgcaagc tgttcgtctg tgttcttgtt atatctttgc tgtattaggt
    37081 aaaaatcgtt atggcagcga ccaatgtcct ctgttttttc ttccaattct tccaccttat
    37141 tatgaagcct gatcctatca attatcgcca taatacctgc tccaattact actataaata
    37201 ctgtttttct tttgttcata ttacccttcc tttcttttag tgtgcattta atacgctttt
    37261 ggccagtgtt ccgctcttta acatcattaa ccctagcaaa accgcatatc caagtatcgt
    37321 aaaggtactt gtgtgtatat ctgttatctg tattgtctta actaatactg cgtatattcc
    37381 aagacaaacc attaaaaaga gtccttgtaa acctatggca aacaatcctt tgatatagtt
    37441 tgttccaatc tgtccccact ctttgtttcc cattgtggca aatggaatgg ctgaaatgga
    37501 tgagtaaaca taaatctcaa acattcttcc gtaaaccaca agcataatca caattgaaat
    37561 gacctgtatt gccaccttta taagcgaggt ttcaaagagt atcatgacaa gctctccaag
    37621 acctttatcc tttaatccat ctaccatttt tactatctga tctccggaga taacggcaga
    37681 agtattgatt acccctgccg ctttatttac catgtgctgt gccacatcaa agaccgccat
    37741 tgaaaactca aatgcatgtg atactaacca tacagcaatc cacatcttga tgatgtactt
    37801 gaaaaattca aaagtatctg tatcgtgcat attgttcttt tgcattacca tattgataag
    37861 ctcgatacaa aggactgctg tgatgattag ccctgctatg ggaataatga cggaatcgtt
    37921 aatgctttta ataaaggcaa atacatctcc gttccacccc ataggagttt ttcctacatc
    37981 tgttgcaact gcaccaactt tatcgttgat gtcaagaaac atggactcta agtttgcttg
    38041 aataccgccc agtagaagct ctttaaagaa ttcttctatc ttgtcgaata ttccaaacat
    38101 ctaagctctc cttttcttat tacttgagta catttgcaag aagcggaatc agcttaagtc
    38161 cgataaggac aatgcctcct cccgccataa gctgcttgat gccctgagat ttcgctcccg
    38221 gattatcatt accatatcct tccataaggt taataactcc ccatgctcca agtccagcac
    38281 ctactgccat aactaaaatc tttaatacat ttactgcctg tgcgaaaaat tccataattt
    38341 attcctcctc attttcttct ttcttctcaa tcttgttata tgacttcact acaaagtttt
    38401 tgtaagtctt tccctctttt tcacgcttct taaagtagcc gaatacatga atcaaatcgc
    38461 ctttttcaaa gtcctctgct ttctctgctt tttctccgta ggctgcacaa ttgatgtact
    38521 ccttgccctt tccatatttt tttacaagcg taaagtttac aacttctact tcttcaccct
    38581 ctttttcaaa acttgagaaa gtaggttctg ccactaagtt ggcgttgatg ttaatcattt
    38641 cttgtttcat ctttttaaat ttctcctttt catttcaata aaaaaaacga ctggagtttt
    38701 tcttttccaa tcgctgatac tcttactatt tagttttcgt ttaagtggga tttctgatcc
    38761 ttatcatctt gcctcctgat tttgaataaa aaaacagcaa atctttttaa gacttactgc
    38821 attcttgtaa taaccgtctc tctatttaat tttgcttttc cttttcgctt catatagctt
    38881 tctatgtcaa acaaattctt cttgtcataa tcctcaagca gcttataatt cttgtgcttt
    38941 gtaatatcgt atttatctga aagaaaaggt ctgacacctc taagctgaaa tatacatttt
    39001 ccaccgtcca ttacagtaat ctcatcttga ctcatcagtt ctttgccaca atgtcaagtg
    39061 atgaattaaa aaaagctatc aaagaaatcc ttcttcgata gcagcgtatt caacatctat
    39121 tcacatttcc tgccttgcgt tcttcgagct gatgaaatat aataagaaat gttgtcggag
    39181 gtgcgaatat ggactacatg acgctcaaag aggcaagcga aaaatggggc gtttcgtccc
    39241 gacagataaa ttattactgc accgatggac gcatccccgg tgctgtcaaa atggccggtg
    39301 tctggctgat ccccaaggag gcagagaagc caaaggacag acggtataac gaaagcaggt
    39361 gatacagtgc tgcatatagc aatttgtgat gatgaaaaag actttgttca gtatttgacc
    39421 gatttactga accaatacag caaggaaaca ggacgggaca taaagattac cccgtactat
    39481 gacggtatgg agctgattga aaaatacgat ccaaccatag acctgatttt ccttgatatt
    39541 caaatgaagg tggtggacgg acttcatgct gccgagcgta tccggcaaat ggacgaaacc
    39601 gtgggcatta tttttctcac tactcttaca cagtatgggc ttgagggata taaataccat
    39661 gcgacgaact acatcattaa gcccatgaaa tatgcccggc tcaaatcaga aatggacaaa
    39721 ttcttggagc gcagccagaa agaagatacc ccctctctgg tgattaccaa cgataccggc
    39781 aaatataagg tgccgctaaa atcaattcga tatgttgaaa catataaccg caatctgatg
    39841 ttccataccg agcaggaaaa tattatctgc tataagagca tgaaagaagt ggaacgggag
    39901 ctttgcgaca aagactttgt gcgctgccat accagctaca ttgtcaatct gttctatgtg
    39961 aaaggcatca agaagctgga cattgaattg attacaggcg agatcattcc catcagccag
    40021 ccgaaacgga aagaatttat ggaacggctg acggactatt ggggggatat gctgtaatgt
    40081 ggttatatca agtgctcgac ataacagcaa cgctgacaga atctttggga ctgtatttat
    40141 tcagtatttg tttttgcaaa gaaccacggt atcgtgcttc tgtaaataga tggcttataa
    40201 caggtataag ttttttgtgt gcgtatacat tgacatggtt ttctgaactt ggcgcactca
    40261 aagcgccttt tcttcttacg atttatcttg tccttttgaa agtttgttat agagattctg
    40321 tctatcaatg tgctgttgct gaagaactga attatattgc aacttgttct cgatattcgc
    40381 aaggtagcgc ggggaaatat ataactcgtc gcttaccttt ttgcggctct cgccgtattc
    40441 atttctcgcg gcttttatag ctgccccgaa agccttaaag tcgtacaatg gtacgggtct
    40501 ttttgccatt ttcattcacc ctgttacatt ttacagttca cctttctttt tggatatgtc
    40561 cacacaattc atatcattag gttaaatact tcctgttgtg tattgacagt agactgattt
    40621 tctgataaaa taaaatttat gtaaaaggag gcggcgttta tgttgcatag catgcgtatt
    40681 agataagcat tgaaaaaaag gattttccca ttttcaacat gggctttttt gtcatgctta
    40741 ttttgcggat gctatacaaa aaactaacga cgtatagata tagtatagac atagtgcaag
    40801 ctatgcctta gttttgttgt actgctctgt gcattttttg ggtagggaaa ccaccggctg
    40861 tgccggtgga ggtaccccgt aaaaaagctt tagcttcaag catcgagcga agcgagctgc
    40921 taacagcaaa ttatcagtga agaaagactt gcattagaac agcggctgcc cgtttacggg
    40981 ctgcggggca aatgatgcgg gtgaaataaa attcagtgcg tcttgagacg catgccggta
    41041 ataggccctt atagggccaa ctcaagccac cggcttagcc ggtggttatg acttgtgtaa
    41101 aaaggacgag tgaaatataa tgcttaaaaa atattggata aaatgtccga tttgtaacgg
    41161 aaagacgaga gttcaagtat ttcataatac tgtattaaaa gattttcctc ttttctgccc
    41221 taaatgcaaa ttgacgcata tcattgatgt agaaaaatta gagattgtaa tcaaaaatac
    41281 agaaaaacaa acttttatta tttagaagaa aggatataaa aatgaaacgt ttacctaaat
    41341 atacgcctgc ggaagtacgg aatgatccat acggatttac ttacaaagaa atgtcggaag
    41401 ttattggcga gaatgaagca aaagccttat atgaagaatt atataagcaa ttaccacgca
    41461 aaaaaaatct atcaatgttg gtaaaaaata tttgcaaaag cagtgatact gaaaagtatg
    41521 tttacgaact gaaagacaac aaatacattg aaacggtttt tatcaagcgg cgagatggtg
    41581 gaactgtttg cgtgagcaca caagtcggtt gtcctgttgg ttgtattttt tgtgagtccg
    41641 ggcgaaatgg ctttgttcgt aatctaacat cgtcagaaat cgtacaacag attatattgt
    41701 tgcgtcgaaa agtaaaccgt atcgttttta tgggtatggg agagccttta ttcaattatg
    41761 acaacttgat aaaagcaatc catattctcc gagatagata tgggctcaac tttccaaccg
    41821 acggcattac catatcaaca gttggtccgg tcgatcaatt aaaaaaattg cgcgaggaac
    41881 atcttaaaat tcagttgaca atatctttac acgcagcaac acaatctgca agaaatcgta
    41941 ttattcctca catgcgcata tatgctattg aagatgttgt taagcaagcc ttatcctatt
    42001 ctgaaaggca taatcgcaaa attgtctttg cgtatttgct tttaccgggt ataaatgacc
    42061 ggccctcaga tgtaagacaa cttgcaaaat ggtttcgggg caaaaaagtt atgattaacg
    42121 tgttacaata caacccaaca agcaattcaa gaattaaagc accacagaaa cgggaaatag
    42181 ttgcattcaa acatcaatta gagcaagcag gacttgaagt tactatgaga gtttctcatg
    42241 gcagagagat taacgcggct tgtggacagt tagctaacac atataataaa ttcaaaaaaa
    42301 aatgattaaa agtgccagac gcatagacgc agagccgata acgcattagg tcaaaaaacc
    42361 tatgtgttat cggctttttt atttaatatt gcgcaaaagc cgctgttccc tattatagaa
    42421 tggattgcgg gtagtgtatt aaagtcgccc ttttttaagg atacggcggt atcggggagg
    42481 tatcgccgtg tccattttta tttactgtaa cccgcctaat gctttgcggt caaaaaaagc
    42541 tatgctccgc aagcgggata ttccggctat gaatgtgaac tgtcaataca tttagctact
    42601 gcttacattt cctttgcgcc cgtccgcgtc ttgcggacgg gcgcattttg cttttttcaa
    42661 cttttttctg aaaagcgcac tcaagagcca tctcccgaac gggtacggag cgaaaggggc
    42721 agagaggaca agcccttaac tcccgtcctc tactccacgc gggaaggagg tgaactctat
    42781 ggagctatct tcttccgaca aggaaagaat acaacatcag tacgacgcat tagcaaagaa
    42841 aactttggtc ggcgaagcga aaagccaccg ccgcactctt gcgaaacgcg cagcacgcga
    42901 agttactttt tcggatttga gcgaaagcga actcgcgcag cttttcacaa cggacgaata
    42961 cgaaagcgat tatttccgtt ttcaagtgtc cggctttgat gtactcgtca aaaatgaact
    43021 gcttgccgaa gcccttaacg ctttgcccga aaggaaacgc gacattatcc ttttgtccta
    43081 cttcttggat atgagcgacg cggaaattgg cgaactgctg aatgttgtac gcacgacggt
    43141 tttccggcac aggaaatccg cgcttgcgaa aatcaaacag tatttggagg gaaaagcaga
    43201 tgatgaatac cgttaggaag tctgaaaatc tgttgccgtt ccctgtcatt tccgcagcgg
    43261 caaacggcga cacaaccgcc atgtgcgcga tcttgaagca ctacgagggt tacatagcga
    43321 aactttgtac ccgcacgctg aaagacgacg cgggcaatac ctattcctat gtggacgagg
    43381 aaatgcgtaa caggctgcaa gtgcgcctta ttacccgcac ccttgctttt catgtaggat
    43441 aatcttttag cccatgcggg gagcgtgtcc cctttccacg cttcccgtta tgggctattt
    43501 gtcgttccgc aaaagcatat ccgctgttga tgtgcttttg caggccgaca aagcctattg
    43561 ttctttgaca aagaaagcgg cctttggtgc gcagcataac aggcaaacgg gtacattccg
    43621 tctgtaccga gccagtcggc gggtacgcca tgacagcttt tccccatagg ggaaaagccg
    43681 agcgagaact accgcgccgt aaaacaggtt tagcagcttg tgggcgacga catacaaggc
    43741 ggcacaatga tactcccgcg ttgaacaccc tcaaagtatt gcgcggcgca cccataagca
    43801 tgggccgggg tgaaactccc gtgaggttgc agctaacaac cgcccgtttt tgccttttga
    43861 cgaatatagt ttatccatac aggaaaagga ggtttttcta atggataaaa aacagacaat
    43921 tacaaccgaa cgcaaaatag ggaaaatcac ctatcttgtt caagcgttgc cgagtgaaaa
    43981 ggcaaccgat acaatccata aaaagattga gaaactcatt gtaaaggatt tgcagaaaaa
    44041 gcccggaaat ccgggatttt tagcgtccga gtagtgcatt aggcggcggg atatggtata
    44101 atgatagcaa cacattatac ctgtttattc ggctgtcgga aaggaggact aatgttacag
    44161 tcgaataaaa tcaccgccct ttactgccgt ttaagtcaag aggatatgca agccggagaa
    44221 agcggaagca tacagcacca aaaaatgata cttcaacgct atgcggacga acaccatttt
    44281 ttgaacacaa agttttttgt ggacgacgga ttttccggcg tgagttttga gcgcgagggg
    44341 ctgcaagcga tgttgcagga agtggaagcc ggacgagtgg cgacggtcat taccaaagac
    44401 ctttcccgtc ttggcagaaa ctatctgaaa acgggcgaac tcatagagat tgtatttccc
    44461 gaaaacggag tacgctatat cgcgatcaac gacggagttg acacagcgcg ggaggataac
    44521 gagtttaccc ccttgcggaa ctggtttaac gagttttacg cccgcgacac aagcaagaaa
    44581 atccgcgcag ttaaacaggc acaggcgcaa aaaggcgagc gcgtcaacgg ggaatatcca
    44641 tacggctata tcccagaccc gaacaaccgc caccacctta tacccgaccc ggaaaccgcg
    44701 ccgattgtca aacaggtttt cgctatgttt gttagcggcg tgcgtatgtg cgaaatccaa
    44761 aaatggcttg cggaaaacaa agtcttgacg attggagcgt tgcgctatca gcgtacagga
    44821 caggcgcggt atcagcgggc aatgatcgcc ccctatactt ggccggacaa aacgctctat
    44881 gacatattag caaggcagga atatttaggg cataccataa ccgcgaaaac tcacaaggta
    44941 tcctacaagt cgaaaaagac ccggaagaac gaagaagaac aacgctattt cttcccaaac
    45001 acccatgagc cgcttgttga cgaggaaacc tttgaacttg cgcaaaagcg gattgctacc
    45061 cgccaccgcc cgacaaaagc agcggagatt gatatttttt ccggcttgct gttttgcgct
    45121 ggttgcagac ataagatgta ttaccaacag ggcgtaaata tcgagccgcg caagttttcc
    45181 tattcttgcg gcgcatggcg caacagggca aggacaggca gcgagtgtac ctctcattat
    45241 atccgcaaaa acgtacttct tgatttagtg ctggaagata tgcggcgggt tttgcggtat
    45301 gttaaggaac acgaacagga ctttatctgt aaagctaccg agtacggcga catggaagcg
    45361 agaaaggcat tagcgcagca gcaaaaggaa cttttcaaag cacaggcgcg tatgaccgaa
    45421 cttgacacgc ttttccgcaa gctgtatgag gacaacgcat taggcagact gacagatgaa
    45481 cggtttgtgt ttctaacttc cggctatgtg gacgaaaaga aatcccttgc cgcaaggata
    45541 gacgagttac aacagcagat cgcaaccgtt accgagcgaa aaagggatat atcaaggttt
    45601 attcagattg tcgggaaata cagcgacata caggaattga cctatgaaaa cgtccatgag
    45661 tttatcgacc gtattttgat acatgaatta gaccgggaaa ccaacacccg gaaaatcgaa
    45721 atccattata gctttgtcgg acaggttgat accgagcagg agccgacgca agttgtcaac
    45781 catgaccgcc gcaacatggt agatgtaaaa agtatcgcta tctaacccca gcaaaaaagg
    45841 ggcctgaaat gaaagagtac acgctaatta tcaagggaga aatggatttt atcatcttat
    45901 ctccgcaggt gctttcttcc ctgattactc aaatccataa cagcccggag cgaaaagttg
    45961 tggtaagtat tgagagcatc atgccaccaa agtttaccga ctatctgctt cgggtaatca
    46021 atagcaaccg ctttagcaat gaacggttcc gctatcgcta cattttagaa aatcctgtta
    46081 caaagaaagg gatgtatgag attttacggc aacagctttc ccgcaccaat acggagcgat
    46141 ttccttgttt tcagaccata caattgaccg acactttcca gggtaatgtg gagctggaca
    46201 tggagtgcaa cgatctgttc ttttgggctt gcaaagatac agcagcaaag tttgtttaca
    46261 cgttcccgga tggacgggag gaaacactgg tgattgaata ctgaatgaga aaagccccat
    46321 ccctcaatgg ttgagagatg gggcaaacta tttacgctgc aatctccggg atttccccga
    46381 caaagttata aacaattctg acttcctgca ccttctgacc gtccaccttc ttaacctcgc
    46441 cgatcaggat tttgctgata agccggttca gtacggactc gtccagttcc gcaatggcgg
    46501 catagcgccg gatttccttg ataaaggttc ggacttcgct ttcctgtgcg tccgcccggc
    46561 tctgcatcac cgccagatcg tgcaggcgtt tctgattggc ttcctgctcc tgttccagcg
    46621 ccgccgtcag cttcataaac cgctgctcgg tgaggacgcc tttggcctta tcggtgtaca
    46681 ggctcaaaaa catcccgtca atttcctgat tccggtcttc cagccgtttc cgttccttct
    46741 ctgtctggga agcgtccacc agataccggc gctccatccg gctgctgagc cgctggtaga
    46801 acgcatcggc gtccttcatg gcctgcgccg ccaattcctg tatatccttc agcaccagat
    46861 tgtacaaatc ccttgcctca atcttgtggc tggtacaggc gttcttgccc agccggttgt
    46921 aagtctggca gatatagtat gccctgtcaa tcggctcccg catttcgccg gtgaagcggt
    46981 tcttcccggt tctgccgacc ttttcatacc gcacctgcat ggacttcccg caggtggcgc
    47041 agtagacaag cccgtggaac aggttataga aggggcaggc gttgcctttc ataatggttg
    47101 gcctgcggtc aatgatgtcc tgtacctgtt cccattcctc cggcgtcaca atcgcttcgt
    47161 ggcagccctc gatgacctcc caatcctcac gggggataat gtcataagtg ttggagcgga
    47221 tgcctttctg gtgtgtccgg cagacaagat gtgcgccttt ataaaaggga tttctcagga
    47281 tatggctgat ccttgcgctc ccccatgaat agtagttcac gtcacattct gtattgcttt
    47341 ttactctggt gatggggatt ttatcatcca tgagctgctt tgcgatccgc atacagcccc
    47401 agccgtccag tgccatatca tagatttttc ggatcaccgg cgcagtctcc gggtcaagga
    47461 tcagatgccc tttgtcctcc gggtcacgca tcaggccaag aggcggctgc ccgccgcaga
    47521 actttccctg ccgggaacgg gtcatgcgtc ccgccagcac ctttttggaa atatcccggc
    47581 tgtacatatc gttcaggata ttcttaaagg gcgtgatgtc catttcctga cgggtcaggc
    47641 tgtccacacc gtccgtgacg gcgatatagc gtacattgtg tttggggaaa aagatttcga
    47701 tatagctgcc agcttcgata taattcctac cgagcctcga taggtctttc gtgatgacgc
    47761 agccgatttt ccccgcctca atgtcagcga tcatgcgctg gaacgagggg cggttgaaat
    47821 tgcgacccgt atagccgtca tccacataat actcatactg gaacatccca tgcttttctg
    47881 cgaagtcccg gagcatgagt ttctggttgc tgatgctcat gctctcattg tcgccgccgt
    47941 catccagaga aatacggcag tagagagcgg tgattttttc atagacttgt tttttcctgc
    48001 tcatagcgtt ctccttctat gaacagggac acgataccat tatagtacca tgtccccgtc
    48061 cctgaatcaa ccgtttttac gcagcttttt ctgccatttc ccgctgatga cgccattcct
    48121 cgaagtgttc gcaggtctgg cgctcaatga gctgctcaaa ggcttcccgc atggacttct
    48181 ccccggagaa ctcccgcacc accacaaaac ggactttatt cttctgattt ttgctttttt
    48241 ccataggcta cctccataga gagacaggcc gaacggcagc accggcaacg aagtccggca
    48301 acgaacctct aaaaacctgt aaactaccgt ctggcctgca aatcttactt tgtgaatttt
    48361 cttgaaaaat ctcgttgctt tcgttgccgg agataatgat aaatattaaa atatccctta
    48421 tttactggct ttttccgtat ccagacaggg aaatccccgg caacgaaccc ggcaaccaag
    48481 tggcaaccat cccggcaaca gaccgccatt ttcaggcgat ccactccttc ggaagctcca
    48541 actggcggca ttcttcctcg ctcagttcca caaatccatc ttcgttgccg ggcagttcgt
    48601 tgccggaaac gtcccgttcc cagcccttct gcctgccgta tccggcgaac atccggggat
    48661 tggagaatgg cttccagccc gtcaccactg tattcatgat ctcgttgata ttgtgaagct
    48721 gccaccgctt cggctcgtca taggtgtggc ccaatgcctc cttaaaaagc tgtttggaac
    48781 agaccatgct gccggtataa tgttccagaa agccctgtat ttgaccggcc tccgtgtcct
    48841 ccggcatgaa atccttctgc acctccacaa gctgccgctg gatggacttg ctgaatttca
    48901 tggaatactt cccgctgcgg tagatggtca tggcctccgc ccagacctgc aacaggtagg
    48961 ctctggaagc gtcctcgtcc tccaaaatgt gaacctccgc tttctccggg taaatcatga
    49021 tgggaaggaa acgccggttc ccggcccggt caaggggcag gaagtccagc gtgttggatg
    49081 aaccgccgaa cacgcactgc cgcagccggt ctttgggctg ggcctcatag ggtgtgcggt
    49141 aggtttcctt ctggcgactg atgaacgagc ggatttcctc tatgcttttg gcgctgctgg
    49201 tggccagcat ctctgacatc tcaatgatcc agtggccttg cagcttcaaa aatacccggt
    49261 catcgtccag ctttttcagg tcatcggaga accactcgtc ccggatcgcc agcaggcgga
    49321 agaagctgga cttgccagcc ccctgtccgc ccaccagaca gagcatttcc tcgtatttgg
    49381 agccgggaga aaacacccgc cggatggctc ccagcaggaa gtgttttaac atttcctcta
    49441 cataatcgct gacttctgca cctaagaagt gatggagaca ggagcggatg cggggcgtcc
    49501 cgtcccacac aaggccgttc agcacgtcct gtatgggatg gtagcagttc tcattcgcca
    49561 cgatggagag cgccgccgtc attttcttct cgctggtcag gccgtagttc tgctcaaaat
    49621 agagaagcag atacttcaca tccgtatccg tcagggcgct ggtgttcctg cgccagccca
    49681 aatcccgcac gatgtccacc cggtcagtca ggaggttcag ccggatggcg tcccgcagga
    49741 gagggtcatg ggagaacacc gtccggcagt tgccgatggt gttggcgggc tggcccttct
    49801 ccgtgacaga aaggccttcc cgcacctcgt caacgctctg ctccggcgct aaagcttcgg
    49861 ctgcgctcat gacggcctgc cgggtggctg gcggtaaact ctgatattcg ctgctcaatc
    49921 ttcctcacct cttttccata ctcagcgacc acgcaggccc gttcctccat actgccggac
    49981 agcagaatat ccagcagata ctccgtgtac ggcttttttt gcagggcctc cacaaacagc
    50041 gggttccatg cttcctccgg cgtctgcggg gcatattcct tctcccagcg ttccagcaga
    50101 tgcagataat cgcacaacac ccggaaacat ttctgttccg cctgccggta gcgcagttcc
    50161 tcgctgattt ttcgcttgcc cggcttcctg cggggcgggt catggccttt agcgtcaaag
    50221 ccgaccgaga aatcctccgc cagcttcaag gccgcatctt tcttgttcag cccgaacaga
    50281 cgggcggtaa aatcaatcac atccccgtcc gcctgacagc cgaagcagtg gaaccggcgg
    50341 tcaaccttca tgctgggatg tttgtcatca tggaagggac agcaggtcat cccgttcctg
    50401 ccgagctgta tcccataaga ttccgcagcc tgccttgtgg ggacagcctg cttcaccgct
    50461 tcaaacacat tcgattttct cacctccaga cagagaaaaa ggcatccggt tttcacagaa
    50521 aatcagatgc cttacaactc tatatcgttc tttttggtcg gggcgattcg cccctcacgt
    50581 tcccaacgct cccggttatc ccggtcagca ttaatttttg cctttgccag aatatccata
    50641 atggattctt tggctttctt cttgacctgc tccttcccgg cttgcttgac ttccggctgc
    50701 atcagcgtcc gctggatttt cgccagagcg tgctgcatgg cgtttttgat tttggtaatg
    50761 accccatcca gacgctcagc ggcatactca cgttccttct ttggagcttt gcgctccggc
    50821 gaaagcaccc attttttcga ttcctccacc agacgaatat cttccttgtg cgtctcctgc
    50881 cggacggtat cggtcaccac ctccaccgcc ttgtcatagg ccgcatcgga aacatcatct
    50941 aacagtgtct ccacgtcctc aattttgagc gtcagttctt ccagcttctg ctcctgcgcc
    51001 gccagttgct ccttctgttt catcaggata taatcctgtt tttccagata gtcccgcccg
    51061 ccgtaggacg gctcctgatc cagatgtagc ccatgccgtc tggcgatgtc aaacagaatc
    51121 gtccggcaaa ccgcatcaaa ggtctgcttg cggttgttgt tccggccttt gggcttctca
    51181 gggtttggga gagggatgcc cagttcttcc agcgcctttt cctgctgggg acacagttcc
    51241 ccgtagcggt tctcacagtc aaagacgtgc cgctcatgga tgtggggcgt cccctcgtcc
    51301 agatggagcg cccagtccag gatgtggata tgggaaccga agcggcgctc aaattcctca
    51361 taaaactcat tgacgatgga aaaaagcgtg tccggcggga cggattctcc catcgtgccg
    51421 atctggtaga tgctttcctc cgggcaggtc ttgttgtttt tcagcaagtc ctccacggtg
    51481 cggttgcgct ccgtgtgccg ggttttctca ttccgggcgt tctgggcctc cacatggccg
    51541 ccgtaatgct cgttgtagta catccgctcg atttcctcaa agctgaaatc cggctgctcc
    51601 gggttctccc ggaactcgtg tgtcgtaaag ccccggtagc agtcccagta gacattttgc
    51661 cttgcccgtt ctgcgtcaat gtgttcgctg ttctccacat cgaaccggcg gtcattgtgg
    51721 cggggattat aggtgccatg cttgccggag cgcccattgt gtcgtgtcag tttcaaacag
    51781 atttcctcct tcctgcgaag atgttctggg gccggggaag gggcggcgaa gccgcaaatc
    51841 ctgcgaattt tgcgtcaggt aatacccagt acgagttgac gcaggcatca actctccctg
    51901 ggcaaggcgt ttcacccttg acccgataat ggctgccgcc cttaacctgc caatgggcgt
    51961 tgccccttga ccccagcagt gcgctgccgc ccctgcaccc cggcacaaag ggctggctgc
    52021 cctctgtact cccgcaccgg cgaagctgga ttccaccgca gaagcggtgt ttgccagttt
    52081 caccggctcc gtatccgcac atttgcgccc ataagcgggg aatggatacg gaatacgcct
    52141 gcggggattg ccggtcaaaa cagcgggtgc agacccggca ttttttccgt aaacgccccg
    52201 gtttcaggcg attttttctt ccgcccctac cacctgaaag ccatgctctt ttgcgtaggc
    52261 gctggcggcg gctttccgtt cctcgctgta cggcggcacc agccggatcg acagccggga
    52321 cttatccagc acataggtca cgctgccctc cggcgtgctg cgctccaacc ggcacagcag
    52381 cgggtacttc cggctgaaat cagccagcct gcgcttcaag ctggcgttga aggtgtagac
    52441 gctggcaagg ttttccgcct cgttccagtt gataatggtt tctttctcat acttagacag
    52501 cttcctcata cagatcctcc tcataatcag tgtaagattt cagcacacgc aggctgcgct
    52561 tgaggcggcg gtactcgtcc atctccatcc gcagatggtg gtaaaaatcc tcgtaccagt
    52621 tttcgcccac ctccgtctcc accttccggg ccagccccag catccggcgt ttggcctccg
    52681 ggtcaaccgt cagtgccgtc agccatttca gccgtgtcac cgtattgtgg tggctgggac
    52741 agccgtaagc gtagagaatc ttcttttctt tcatactcag tgtcataatg atttcctccg
    52801 tttctttgct gcggagccat gcgccccgca tgattgataa acttgtttct gtctgccggt
    52861 ctgcctgcaa gccgctcctg cccggaattt ctcccggcgt atcggcctct ttggtgcgct
    52921 cgatttctgt cctggcgtca cttccccgga ggccataccc cttgcagccg gtcattcgat
    52981 tttcaaggtt ctgtgcctgt ctgcaagaac cagtttaccg aaaaaagagg ccctttcccg
    53041 tatatccaaa acgtcggaaa aacgcttgaa atccgcatat ttccacgctt atggaatcgt
    53101 gctataatgg gtgtaatcac tgaaaaacga taacgagaga gggggctttg gatgtacgca
    53161 aagctgacaa tcccggagcg gctcaaagac ctgcgggtgg tagataagca cctgacgctg
    53221 gaacaactgg cggagcagac cggcctgtct aaatccgcgc tgggaaaata cgaaaccgat
    53281 gactacaagg acatcagccc gtttgcgatt gcaacgctgg cggactttta cggcgtgtcc
    53341 accgactatc tgatggggct gacggaaaat aaaaatcacc caaacataga acttcaatct
    53401 ctgcatctga gtgatgatat ggtggaactt ttgagcagcg gcaaaatcaa caaccggctt
    53461 ctctgcgaac tggctaccca cccgaatttc cggcggctga tgatagatat ggaaatctgc
    53521 atcgaccaga tcgccaatat gcgggtggag cagatgaatc tggtgttgga ggccacccgg
    53581 cagaccgtca tgaacaagta tgcacccgga gaagatgacc tctatatgcg gacgctggaa
    53641 ctggggcagg tgcaggaaag cgatttttac agccacgtca tgcacgatga tctggacagc
    53701 atcgtccggg acatccggga agcacaccgc aaagacaaga ccaccgccga cccgcagccc
    53761 acgctggatg atgtgaagga aaaatttgag caggccatag ggcagggcag cgatacagaa
    53821 atgctgatcc acgagttctg cgataagttg cagataccct ttgagaagat ttcctcggag
    53881 gacttctcgg cgttcctgcg gatactgagc ctgtccaagc agctcaaaag ctccaacaat
    53941 atgaggggaa aggccagccc cattcctccc aaccgcaagg ccagacgtaa gcggaagtaa
    54001 aatttccata tccggaagcg gcaaaaaagc catccgccga ttgattggca aatggctttc
    54061 gtgaagctgt attctgttgt agtggttgtg cccagtttgg gaacagtcac tattgaagat
    54121 gctattcttt gtctttctgc gatacctgtg catgggcttc ctgctgaaca agcagccccg
    54181 cagaaaagcc atatttgaag tggctttcgt tttcctcaca ctgggcgatt gccacctgct
    54241 cccgcagttc ttcaaccagt gcatagtcct ctttgctcag acgctccgat aaagtgtcca
    54301 tcagggcggc gctggctttg actgcctgct tgaacctcgg tgaatcagcg accaccgttt
    54361 cacagggata aaatctgccg ttatacattt cttccagaat catgcaccca cctccctcag
    54421 acaaaaatca tctcagacag gatgatttcc tctacccggc tgcggatgct gttcatgccc
    54481 ccgacccatg caagctggtc acgggctttc agttcctcgg tgacgccctc cgcagcctgc
    54541 atctgcgaga tgatacacgc cagtcggtca tcggcctgct cgttcagatc ggcaagatac
    54601 gtccagagtt ttcccgacag aagcagttca ttgtactggc cgggacggtg ttcttgcaga
    54661 aacgccttgt gtatccgacc ccaccgcccg atggggcgga tttcctccga caactttaga
    54721 tcggggatat agtagtctcc caccagcgta tagctgatgc cgttgtgatg gattttcttt
    54781 ggtaaatgct ccatgttcga cctcctgtat tcagtttttt cagaatccag ttgatcgtgt
    54841 ccctgttcat gtgaaattca aggcaactga actcttcacg aacaagtatg gcatatcctt
    54901 accagtcttt tgatagttta aaccaaagct cttttgattt gaccttgttt ccgacgtgtt
    54961 atacaggtcg atagtttctt ttcccagtgt ttcagacaat tcttttagcg tagttttctc
    55021 cttgccacca agaaacaagg tactatcaca gttacctacg attgtatcag cgttatcctt
    55081 gtagattgcc tttagctgag attgtgcttg aagtatgata cttgctgaaa tctctcttga
    55141 acggattgtt gcaattaact tctcaaactt aggaattaag ccgatattag cgaactcatc
    55201 aagtagacac ctcacatgga caggtaatcg tccgccatat acatcatcgg ctttatcaca
    55261 taaaagatta aataactgtg agtacataat ggatactaca aagttaaatg tatcatcggt
    55321 atcggagata ataacaaata gtgctgtttt tctatctccc agtgtatcaa gctcaagttc
    55381 atcgtcactc atcaagtccc taagttcctg aatatcaaag ggagcaagtc ttgcaccaca
    55441 agaaataaga atcgatttgg cagtttttcc cgcagccaat ttgtactttt tatattgctt
    55501 cactgcaaag tgtgtaggtt ctttcttctc cagtgcttca aagagcctat ctatcggatt
    55561 catataggtt tcattatctt ctctgacttc tgaagcatca atcatatcaa gcagagttgc
    55621 aaagttcttt tcttctcttg gagcttcata gaagatatag ccaataagtg ctgtatagta
    55681 gagtttctca gctttcaccc aaaaatcttc acctgatttt tctccctctc ccttggtgtt
    55741 tgcgataatt gtctgtacta atttgagtat gtccttttca cttctaagat aagcaaaggg
    55801 attgtatttc atagactttt tgaagttaat ggtatttagg atttttatct catatccgtt
    55861 atcttcaagc atcttaccgc actcaaggac tatagttcct ttaggatcgg tcacacaata
    55921 tgacgagtgc atctgcataa ggttcggttt tacataaaat ctcgtttttc cacttcctga
    55981 tccaccgatt acaagtacat tcttatttct tgcatacttt ggatttgccg gtctgccatt
    56041 catggttaat cgttctgttt gcgtaagtag gatattgttt tgaaactttt catccatata
    56101 tggctctata tcttttttcg ttccccatct tgctgagcca tactctttcc cctgtctaaa
    56161 ctttttagca tttttccctt tggtatatac gataattttg attaaaacag ctactaccac
    56221 tcccattaaa atatccgtag gatgaatgct tggaagaaag ctcatggtgt taagttccag
    56281 tataccttga aatattttgt ctattacatc gccaccagta tatgctctta catgatgaga
    56341 aaagatattg ccaacataga aaaatgcaag atagggaatg ttctgcttta gaaactttgc
    56401 cttatcttgc accttaaata agcctttgat gtcttttagt atcttatcta tcataggctt
    56461 tgctcctttt gtttattttt gattttctct tttgaaatag agtttttagc catctctttg
    56521 aacttctcaa tattcttgtg aatagattct ttcctttctg ctttcttttc tgattttgaa
    56581 agagcgtgct taaaagcttt atccatcact ttcatatctt tggcttgaaa gaatacagag
    56641 tttttacctg tctctttatt tttcatcact gaaaatttaa caccgtatct attcaactct
    56701 tttttaagtt ctttaagctc agcttcttcc actgggattt cttcaagctg tcccttttta
    56761 accatatctt ttagctttac ttcatttccg ttggcattga ccagtttttc aagtcctccg
    56821 aatttctccg cttctttcat caattttttt aataagttta atagaacttt tccagtaact
    56881 ttaccagcat ttatctccat atttaaggtc tttcttgcta tttcttcatt gatcacatca
    56941 atatcctcct ctcatatcat agcttacaag agccgaataa taggcatcta ttccacttgg
    57001 tgcattataa gcggcggtca ggacaaaagc tcttatattt ctaatcctat attcattttc
    57061 tcttaatact cctactaagt actctaaatg tcctgaatta agtttcctaa atctttcctt
    57121 tactacttgt acagatatct ttgtttggtt gatagtaatg aaagattcat catctaatac
    57181 cattacatct gccagtatct ttaatatttc atcaaactct tcagccacac tcttattccc
    57241 aatactcata tgttcataat atcctgtttg gtgtcgcaat tcttctaaac aattttgata
    57301 tatattttta tagcttatat ctatcctccc tcccttcata ttctctgaaa ataattgatt
    57361 cttttctttg atggaaggat ggatggaaat atatgctgaa ttatttgtag tattatttgt
    57421 agtattattt gttgtattct ctggtaatgg ttggtgcaaa ttatcctttt gcattagtgc
    57481 attttgaacc tgtgcattag tgcaatctgc attattgcat tggctgattt tgcccttatc
    57541 cattggtgca ttttgcccta atgcattggg catagaattt tttaatgctt ctgtttgtat
    57601 tttttgtttt ttcaaatcca tatccatata taattcttca agtttatcat tattaattgt
    57661 ataccatttt gttttatctc ttggatcctt attgtaattt ccaactaaaa gataacctgc
    57721 tttttctaac tttgaaaaag ttctttttac tgtatcaaag ctcatatagt caaagtcatt
    57781 ttcttgccat gctcgaattg aattatatgt ccagtattta ccatcatgat aattctttcc
    57841 tgatttttta tttatttcta tccaataatt aatctgctgt agtatgagtg cttcgtttaa
    57901 tcctatttct ctagcaagag ttttattcgc aactataggt tgttcatcaa ataaatacat
    57961 aactcatcaa acactccttt ctttttttca aacaaaaaga cgatagttat ttctatcgtc
    58021 taaaatcata tataaatatc attttctaaa tagagcgtat tgcttctttc actcccgtta
    58081 aaacggctat aacagctgtt ccaaccattg gtattccgta tgggcttaac aaaaatccta
    58141 atatcaatgc ttctatccct atggtaacct ctttttgtaa aaaagatgct attgctccaa
    58201 atatgcaaaa catcatcaac aggtaaagta ttgctgttcc tatgccaagt aaaaatgaaa
    58261 gaaatgcaat gagaatactt aacgctatac taattggaaa taagataatt tttaatatcc
    58321 atctcatata tactctccat ttatactaaa tcattttaaa atgtcttaaa gcgtccatta
    58381 tagcttcttc tttttcaggc gtacactgtg ggatagcctg attgtctttt ttagatttat
    58441 tataatgctc tctcagttca attccacatt tcttcttaat ctgtgcaata taaagtgtag
    58501 aaacctttag cccaaatttt tctaatacat actccttaat ttgagcatat gttgctttgc
    58561 tctctgcact tgtcaaatca agctcatcca gcttaatttc aacatctata tgcttatcga
    58621 catcaagttt ggacaataac gctaccgtct ccacatgtgt agaatgaggg aacatatcta
    58681 caggttgtgc ctcaacacat ttaaatccgt gaacatttag atagtctaaa tcccttgcta
    58741 aagtagaagg attacaagaa acatagacaa ttctttccgg attcattgaa acaatagtct
    58801 ctaatacctt ttcgtcacat ccctttctag gtgggtctac aactactaca ttggcagtct
    58861 taccctctct atacatctta ggtacaattt cctctgcctt tccaacataa aattcagcat
    58921 tatgtatatt gtttaattta gcatttatct tagcatcttt tatggcatca tctataattt
    58981 ctattccata aaccttttta gccttttgtg ccaaaaataa agatattgta cctatcccac
    59041 agtaaatatc aaaaacagta tcagtatcat ttaaatttgc atattcaaga gccttatcat
    59101 acaatacttt agtttggaca gggtttactt ggaaaaatga taatggtgaa atctcaaaca
    59161 ctaagtcacc tatataatcg tttatttttc catctccata tataactata ttttctcttc
    59221 ctagaatagc attcgtatct ttttgattta catttacaac caaagtctta aatccaggta
    59281 tattttcctt aagcactgaa gctaattctt taagatatgg aagtttgttt ccatttgcta
    59341 ctaatacaac cattacttct ttagttgtaa aacctatttt tgttactaaa tgcctaatca
    59401 accccgtgtg agtcttttca tcatatatac ttaccttata ggcccttata tatgttttta
    59461 ttattttaac aattttatca tttatctcat gttgtattac gcatttttct gtcgatatta
    59521 catcatgagt cttcttttta tagaagccaa taattggaat accattattc ttttgtattg
    59581 gaaattgtgc tttatttcta tatctatatg gattatccat tcctatagtg tcatgaactt
    59641 ctacatcctc taacttacct attctcgaaa gtacttgttt aacttcattt gttttcattt
    59701 caagttgttt attataatct aaatcttgta tctgacaccc tccacaatcc ctaagattgt
    59761 cactacacac tcttttcacc ctgaatggag acttttctat tatctcaaca atatctccct
    59821 cagcataatt tttctttact ttattaatcc taactttaac tttatcatgt attaatccgc
    59881 cctctacaaa aactgtaata ccattgtact ttcctactcc tactccgcct tgaccaatgt
    59941 ctactatatc tacaatatac atctcgtttt tcgaaagcat aaatcaccct ccaatcaatc
    60001 tattatattt aaatctttac aaactttttt ctttaaattt atattaaaat attgctatta
    60061 attaaactat attttttact aagctacaat attttatttt accaaaaaaa aaggagctta
    60121 taaagcccct tagtaaataa atttattatt tttcttataa aactctagtt gaacctctat
    60181 ataccacgcc tcttgatgca tccacagtta caacttctcc atcttttact agatttgtta
    60241 tatcagttgc agacacaata actggtatat ctaaatttat acctacaata gctgtatgtg
    60301 atgtcattcc accattctca gttactacag ctcctgcttt ttctatataa ttgttcattt
    60361 cagcatcagt agcagtagaa acaagtatgt cgccttcatt aaagtctaca caagcatttc
    60421 cattctttat tatacgaact ttaccttcta ccgtttgagt acctacacct ataccttgca
    60481 cgatttcttc gcttattaca tgaaccttta ttaaattagt agttccactt actcctactg
    60541 gtactccagc agttataact actaattccc cattatctat ataatcagct tgtctagctg
    60601 cctcaattgc cttttctata acttcatctg tatttcctgc aacagaagac ttaactggat
    60661 atactcccca tgtaagagct aatttattca tagtcttttc attatttgta gtagctatta
    60721 ttggagattt tggtctaaat ttagatacca ttctagcagt atatcctgaa gaagtagatg
    60781 ttattatagc tgatgcattt aaatctactg ctgttgtaca agttgcatgg cttattgcat
    60841 ctgttacagt aacattgtta gtaccatttt cctttagtaa tctatcataa tctaaagtct
    60901 cttctgttct ttttgctatt gtagccatca tttttactgc ctctacagga tattttccag
    60961 cagcagtttc tccagataac attatcgcat ctgttccatc atatatagca ttggcaacgt
    61021 ctgtaacttc tgctcttgtt ggtcttggat ttcttatcat agaatcaagc atttgagtgg
    61081 cagtaacaac tggctttgct aattcattac atttttttat catcattttt tgaactattg
    61141 gcatctcttc agttggtatc tcaacaccta agtcacctct agccaccatt ataccatcag
    61201 aaacttttaa tatttcatct aggttctcta caccttcttg gttttctatt ttagatatta
    61261 tttgtatatc agttgcatta ttgttctcta atacttctct tattgctagt acatcagatg
    61321 cctttcttac aaaagaagca gctatataat ctattccttg acttattcca aactctatat
    61381 cacttatatc tttaggagtt attgctggta aatttatctt aacaccaggt acatttactc
    61441 ctttatgatt tttaactatt cctgaatttt caacaacaca aactatatct tctccattta
    61501 tttcttttac tcttaagcca actaaaccat catctattag tatagaatct cctgatttaa
    61561 cgtcttcagc aagaccttta tagcttactg tacacatttc tttagtcccc ataatatctt
    61621 tcatagttat agtgaacttt tgcccttctt ctaaaaatac ctctgggtct tcaaagttac
    61681 ctgttctaat ttctggacct ttagtatcta ataatatagc aactggctta tttaattttt
    61741 ctctaacttt ctttgccata tcaattcttt ctttatgttc ttcatgcgaa ccgtgagaaa
    61801 agttaaatct acacacattt agaccatttt gcataagttg agttaaaatt tcctcacttt
    61861 gtgatgctgg acctagtgta caaacaattt ttgtcttctt tacattattt aacatatttg
    61921 ctcctcctga gatattattc cacagattta tttttaatac taaaacttat atagataata
    61981 tttttgccat ctcataagct tctttatcaa atactttctt ttgagctaaa gcttcactta
    62041 tttctaaatc aattattttg ttttctttta ttcctacaac tctagctgat tttccatcta
    62101 ataataattc tactgctctt actccaagtc tacttgctag tattctatca gatacagttg
    62161 ggcttcctcc tctttggaca tgccccaata cagtgactct taaatctgct cctgactctt
    62221 ttttaagttc cttttctaaa tcactagcac ttccgactcc ttctgctaaa acgattatac
    62281 tatgtctctt acctcttttt tgagtagtct ttaatcttag agcaacttca tctactgtaa
    62341 tttctacttc tggtactatt atagtttccg ctcctcctgc aagacctgca tataaagcta
    62401 aatctccaca gtgtcttccc ataacttcta ctatgttaac tctttcatga gatgatgatg
    62461 tatctcttat cttacctata gcatctataa tagtattcat tgcagtatca aatcctatag
    62521 tataatctgt atatgctaaa tcgttatcta tagttcctgg tatgcctata gctgggaatc
    62581 ctaagtcact aagtttttgg gctcctgcaa atgaaccatc tccaccaatt acaactaagc
    62641 aatcaatttt atacttttta agaattttta ctgcttttaa tctaccttct tctgttttaa
    62701 attcttcaca tctggaagat tttaatatag ttcctcccct gtgtataata tctccaacag
    62761 aagataagtt catttcagtt aaatcttctt ctagtaatcc cttatatcct ctattgattc
    62821 catatacttt gcatccataa tatattgctg atcttacaac cgctctaatc gcagcattca
    62881 ttcctggagc atctcctcca cttgttagta atcctatagt tttcattttt tacctccttg
    62941 gattttatgt caatttttta ttataatcat tgttattgta ttctaatgga ctttttttgt
    63001 ccctatatca ctttttaatc ccatcaagac taaaaaaatt aaatgtcctt aaatgtccat
    63061 tttgtgatat tatatcacaa aacggacaat atttttcata ttattataat atatttagat
    63121 ttttatataa aaaatcaagc ttttactttt atgttatctt taggaagtaa ctcttctaag
    63181 ttatttataa attcatcaca tatacaagtc aatgccccgg tcattttata tgctttacgt
    63241 ttaccattta tattttcatc catagggtac aagtatatac tgtcattacc tggatattgt
    63301 ttggctattt ctacaatatt attagttgta tcagtatcag acatactatc tattttcaaa
    63361 tacaattttt taccactttc tggcttaata ttttctacat ttctagttct tcctgaattg
    63421 tagtttctat ctgcaaagtc agtatcatca tttatatctt ttatctctct agctataagt
    63481 tttgcatttt cgccttcttt tatacttaat gttcctttta catatattat attatcttca
    63541 ttcaaaatga cattatactt ttgtaataat tgaggaaaaa ctattatttc tatagcacca
    63601 tacaaatctt ctaattcaat aaatgccata atttcatttc gttttgttgt tttaatagtt
    63661 ttagttacaa tcattccacc cattataaca tctgtttcat tcatttgtag gtatgactct
    63721 tcttcctctt ttaatgaatt taattttcca ttatccatag atgttttttc ttgtagttct
    63781 ttttcaaatt gtgataaagg atgtccactt acatacattc ccaagacttc tttttcaagg
    63841 ttaagtctct ctctttcttc atactcttgc cttttaggta aactgtatat ttgttgaaaa
    63901 tcactatctt cctcactatc tccaaaagca tcaaataaag aaatttgacc ttgaacattt
    63961 ttctttctat ccatagatac actttctaat aaactttcat aaccagccat cagactagct
    64021 ctattttcac ttatattatc aaaagctcca cattttatta gactttctat tactctctta
    64081 tttgtatctt tttggtctaa tcttttgact aaatctacta aatctacaaa taatccatta
    64141 gattctcttt cagcgattat attgtttatt atatttacac caacattttt tacagctgct
    64201 agaccaaatc ttatattgtc gccttcaact gaaaactttg aaaaactttt gtttatatct
    64261 ggaggtaata catctatttc tagtgcttta cattctctta tatactcaac taccttatca
    64321 gtatttccca ttacactggt tatcaatgct gccataaatt caactggata atatgttttt
    64381 agataagctg tctcatatgc taaaacccca tatgcagctg catgtgattt attaaatgca
    64441 tatctagcga agtctatcat atcatcaaat attttattag ctatttcctc ttctacacca
    64501 tttctgatac accctgcaat ttctatatta ccatcttcat caaatttacc atgaataaaa
    64561 tattgtcttt cttcttccat cacgtccatc tttttcttac tcatggctct tctaactagg
    64621 tcacttcgtc cgtaactata accacctaaa tctctaacga cctgcataac ctgctcttga
    64681 tataccaaac aaccatatgt aacttccatt ataggcttta atttttcatg tagatatgtt
    64741 acatcttttg ggttgctttt attttttata tatgcaggta tagaatccat tggacctggt
    64801 ctaaataggg atattcccgc tacaatatct tcaaaattgt ctggttttag ctgtttcata
    64861 aaacttctca tgccagcact ctccaattga aacaccccta gtgtatttcc tgatgaaagc
    64921 atttcatata ctttcgggtc gtcataatcc atctttgaaa agtctatttt ttctgtgtag
    64981 ttttttataa ctctattttt ttctattaaa tcaagagtat ctcttataac tgtaagagtt
    65041 cttagcccaa gaaaatccat ctttaaaagc ccaagttctt caagtgttgt catagtaaac
    65101 tgtgttgtga ttgcatcttg atgtttgtac aaaggtacat attcatctac gggatttttt
    65161 gatataacaa ctcctgctgc atgagtagaa gcatgtctta gcattccctc tatctgtttt
    65221 gatatattta ttatttcttt tgtttccggg tcttggtcat acaattcttt aagattagga
    65281 tttgtatcta gggccttatc tattgtcatt cctaaagcaa atggtatttc cttggctacc
    65341 ttgtcaactt tattgtagcc tatatccaat acccttccaa catctcttat tgcagctttt
    65401 gcacccattg ttccaaaagt tattatctgg gctacatggt catcaccata ttttctcttt
    65461 acataatcta taacttcttc tcttctctca taacaaaaat ctatatctat atctggcatg
    65521 gatatacgtt ctggatttaa aaaacgctca aataatagag agtactttat tgggtctata
    65581 tcagttatct taagtgtata tgctactatc gaaccagccg cactacctct acctggtcct
    65641 actataatat tattttcttt agaaaaattt ataaaatccc aaacaattaa aaaatactct
    65701 acataaccca ttttttcaat tacatctaat tcataattta atctctctag tatctcattg
    65761 ctaggattat catatctctc ttttaatcca ttaaaacaca actcccttaa ataagtttct
    65821 ggtgtatatc cttctggaac atcatactta ggaagatgta tagtattaaa atcaaactcc
    65881 acattacaca tatcagctat tttcacagta ttgtcaatag cctctaatgc gtatggaaat
    65941 aattcttcca tttcttctct tgattttaag taaaactcat cactaccaaa tctcattcta
    66001 tttgggtcat ttaatgtttt tcccatttga atacacataa gaacatcatg tattttcgaa
    66061 tcttctttat ttacatagtg aacatcatta gtagcaacta gtggtatacc agtctctttt
    66121 gataatttta ctaaaccaga gtttacttct ttttgctcag gtaaattgtg atcttgaatc
    66181 tccaaaaaga aattttcttc tccaaatatg tctctgtagt caagagcaaa ttgttttgct
    66241 ttttcataat ttctattcat taaagcacgt gctacatctc ctgcaaggca tgcagaaagt
    66301 gctataatcc ctttgttatg ttttttaagt tcttccatgt caactctagg cttatagtaa
    66361 aaaccttcta cgtatgaagt tgaaactatt tttattaaat ttttatatcc ttccatattc
    66421 ttagctaaca gaactaaatg tccttgatat ttgtcatagt ttgggtcttt atctctcaat
    66481 ccacgtgcag ctgtatagac ttcgcaccct ataattggct ttattccttc ttttatagct
    66541 gttttgtaga aatctataac accaaacata caaccatggt cagttatagc tattgagctc
    66601 atgtttagtt ccttagccct ttttataagt tttttcaccc tcgaaaaccc atcaagtaaa
    66661 ctatactcag tatgtacatg aagatgagca aaatctttgt tcattaaatc acctcctgct
    66721 ttataatttt aattttacca caaatatatg tcctaaaaca aaaacagaga cttttatctc
    66781 aaaataggaa ttaacttcaa catataccat tatatgttat aaattaatgc ttattctgag
    66841 attgtcccct caatttattg ctatgatttt tttttcaaat ctgttgcaat ttcttctatt
    66901 tttctaagtc tatgattgac tccagatttt cctattggtg gttctaacat ctctccaagt
    66961 tctttgagag tcatatcctc atactcgatt ctaatcttag ctatctcttg taaattttct
    67021 ggcaaactgc taattcctat agtctcttgt atgagcttaa tattttctac ttgtcttact
    67081 gctgcattta ctgttttaga aaggttagct gtctcacaat ttactatcct gtttacatta
    67141 tttctcattt cttttactat tttagtattt tgaagactta aaagagcgtg gtgtcctcct
    67201 ataatactca acaaatctga tatttgttca ctctctttga tataaacaac gtgattattt
    67261 tttctagcca caattttact attaaaacct aaagagttta taagttcttt tagagaatct
    67321 gcaaactcct cattattagt tacaaattcc atatgatagt tcttttctgg gtcgcttata
    67381 gaacctccac caagaaaagc tcctcttatg aaagctctct tacatccttc atgcttaatt
    67441 aagttctctg aaattttgtt tatagtaaaa aagccttctt tatcttcaag tatacctaac
    67501 ttttttaata acatttctga acccatttcg cttgttacca ttaatacata actgttattt
    67561 ctctttagca tttgattctt atttactgag attgtagtat tgatattaaa attctttttt
    67621 aataatttaa atactttacg ggctattgaa tttaactctg ttgttatctt tagatttagc
    67681 ttcttatatc caacgatttg tatacttccc cctgatttaa ctagagctga taattcagct
    67741 atattacaac attcattccc agatataact cttgctagtt catttttggt ttcagttgaa
    67801 aaagacatat ctatcaccta ttaactaatt tttctttaac ctttttttta tttcttgtaa
    67861 ggcaaccttt attatctcct tttcatcatc atacctagct aggtctacca ctctatctag
    67921 atggataaat ttagcatcaa taaagccttc ctctttttgt ggaatagtat ccatattctt
    67981 tgcttgcatc aaataccaaa aaactgtttt atgaactgct ctattttcat cccagttttc
    68041 tttgaatgta tagtgtattt cacctaaata tttcaatata tctgctttta ctccagtttc
    68101 ttcacttact tcacgtaatg ctgcctcttg gttattttcc ccttcttcta cttttccctt
    68161 gggtaacacc caatctccat tgaacttcct tagtaaaaga atcgtattgc caaacaggac
    68221 tacaccacca gcactaacct cttctctcat cgatgttcac tccttaaaac taacataatg
    68281 ataaggattt ctacacaatt ttttaaaatc cttttttact aacttttgtt gtaattatga
    68341 tttagggcta actcaataac tatgttagat atatactttg catcatgtct tatataatta
    68401 ttttttattt caattaaatt cttctctacg gtttttatac ccatctcttt taatttatct
    68461 ttttgctttt tatctaacaa gacttgctct gctccatctt gtttatataa gtcaagcatt
    68521 tcttctggca atatctcatt atttgcgatt acataatcta ttaaattctc atcagtgtgc
    68581 tttactatag cattaacatg ctctaataca ttgtaccctt ctgtctcacc tggttgagtc
    68641 attatatttg ctatatatac ttttggagct acagacgatt taatagcgtc tacaatcccc
    68701 tctacaagta aattgggtat tatactcgta tataagcttc cagggcccat aattataatg
    68761 tcagcatcgt aaatagatgc aataacatcc tttaatggtt tgacatcttt tggatttaaa
    68821 aaaattttat caatagagct tttttgaatt tttgactctt caggtatact agattctcca
    68881 tttattatat tgccattttc taatttggca actaaattta catcttccaa agttactggt
    68941 aaaactcttc ctgttatagc aaaaatttca cttaatttat atacagcttt ttcaaagttt
    69001 ccatacaacc cattcatagc agccaaaaac aaatttccaa aactctgacc ttttaaaagc
    69061 ccttctgtaa acctatattg cataacttca ttcattgttg gctctatatt tgcaagtgct
    69121 aatagacagt ttcttatgtc tcctggtggg agcattccta agtcttctct caatacacct
    69181 gaacctccac catcatcagc aacagttaca attgcagtta tattttgcgt agtgtgttta
    69241 agacctctta aaaatactga ttgaccagta cctccaccta tcacaaccac atttggttct
    69301 ctattttcaa caatagacct ttgaaatagt tttacttttt cattttttgc ttttttatat
    69361 ataaataacg atacaattaa tgcaatccaa cctaaaattc ctacaataat aagtaattta
    69421 gccatgtttg cctccattat cgcaacatag agtctctatg tttctttaca actctatatc
    69481 ccttattaga taaatcctcc gctataagat ttgttatagt aactgaccta tgtcttccac
    69541 ctgtgcatcc aacaccaatt actagatgtt gttttccttc atctatatat tgaggtacta
    69601 aaaaatgaat catatctaaa agtttcacat aaaattcttc actgatttta gaattcataa
    69661 cataatctct aacttcttta tcatcaccag ttttagctct taactcttca acataatagg
    69721 gatttggaag aaatcttaca tcaaatacta aatctgcatc agcaagtata ccatgtttaa
    69781 atccaaaaga tactacagaa atagttaggt ttggattatc ttcaccagaa gaatatattt
    69841 tctttatttc ttccttaagg tctttaggtt tcatgttagt tgtatcaatt atacatgtag
    69901 ataactcctt taaaggttcc attatctttc tttccatttt tataccttct ggaatctgca
    69961 tatcttttgc caatggatga tttcttcttg tcattttata tctctttaac aatacatcat
    70021 cattacagtc caaatatacc atttcatatt cataattttc tttttctaaa taatttaagc
    70081 tttcatgaag cgcttcaaaa aactttctcc ctcttatatc tatccctaga gcaactttat
    70141 caatacttga atttggttga taacataatt ctgcaaattt agttatcaaa gcaggaggta
    70201 gattatctac acaataaaat cccatatcct ctaaagctct cattgtctca ctttttccag
    70261 atcctgaaag acctgtaact ataacaaatt tcattgctaa ctcctccctt ttatcttatg
    70321 tttattatat tataatgttt tctactaacg atatatccag attagagtca gatatagtct
    70381 tcatagctag tttaaaatca ccaactcttt ctggacaatg tgcatctgag cctattataa
    70441 atttattttc tagccctttt attttattta attgatttac attcaaaaaa gaatggctac
    70501 tatttatttc aagtctagta tttgtctttt ttgctgtctt tgctacttct tctatataca
    70561 catcgccttt atctcctgga tgagtaatta taaaaatatc attattactt atcgcattta
    70621 ttacagcttt tgtattgtat tcttttgctt tttcagtttt aaaaatatag ttattacaat
    70681 gatttaataa gcttcttaat gatgttggag tagaaccaaa atgatatcca gccataatat
    70741 aatcaaaatc tctaataaat ttatcatcca tatctatatt gcctttatca tctaatatgt
    70801 tacactccat acctaaaagt atatttatat cattgtactt atcatttaaa taatctactt
    70861 cttctctcat tttgagaata tcttttttct ttacaccata tgcaatatgt ttatagctat
    70921 ggtcagatat tcctattgtt tttattcctt tagaaatggc tacctttaca ttatcttcaa
    70981 tagtcccttt gccatgacta tataatgtat gtgtatgata atcagccagt attttcattt
    71041 gttaatcctc tccaagtatt ttgacttctt cttccaacat aaccccaaac ttggcatata
    71101 cagcactttt tataacatat attaaatcca aaatatcttt agcacttgct tctccttgat
    71161 tgactacaaa tccacaatgc ttctcagata cttgtgctcc tcttaaagta agacctctca
    71221 atccagtttc ttctattaat ttacctgcaa aatatcctgt tggtctctta aatgtacttc
    71281 ctgcactcgc aaaatttaat ggctgtttag ttattcttct tgtcgctaaa tcatccatta
    71341 aactttttat ttcatcataa tttccttctt gtaattctag ttctgctgat aaagctatat
    71401 atccgttttt cgatattata ctttttctat acccaaattc catctgttca tttgataatt
    71461 caaatatatt gccttccata tccatcagtc ttactgactt cactatgtcc ttcatttcac
    71521 caccatatgc tccagcattc atggctagtg ctcctcccaa tgtaccaggt attcctgatg
    71581 caaactcaaa cccttttaat tcttgctttt ggagagcttt acctaaaact gataataaag
    71641 ctcctgactg agcagtcatc ctagtatcat ttatctcata agaattgaaa ttatcagcta
    71701 attctattac cactcctctt attccaccat ctttaattaa aatgtttgaa ccatttccaa
    71761 ttattaaata cggaatagat tcttctttta ccaacttaag tacatttttt aattgttctt
    71821 cagttctagg tctaactaat atatctgctg gtcctcctac cctaaatgaa atatgttttt
    71881 tcataggttc atcgactttt atatcctctt tacttaatat atttaataaa tttatatata
    71941 tatcttgatt attcattgta tttcctccca aattatactg aactttagct gtcattatta
    72001 tttaaattac attagtatat agtattaact atattcaata atatatgatt ttgcttatca
    72061 tatggatttt atattttaag cttcttaatt acaaactata ttcaataaat atataataaa
    72121 gtaaaaaaaa aaaaataaca acatataaaa gaatataaat aatttaaaaa aagcatcaat
    72181 atcactgata tcaatgcttt aaaaggcaat ttaattcgta ttatttatct aactattttt
    72241 gacatagcaa agataataaa tccctccaac tactatggaa cctccaacga tatttccaag
    72301 cgttacaaat accaaattag ttataaattg aaccatagtt atatttgctc ctaatgccat
    72361 tcccattggt ataaaaaaca tgtttgcaac actatgttgg aatccgcata atacaaataa
    72421 cattattact gtccaacatg aagccaattt tgacattata tcttgagatg ctgtagccat
    72481 ccatacagct aaaacgacta aaatattaca aagtattgct tttaaaaaca ttgatgctat
    72541 agttgaatta accttagctt ctgccacagc tatagctttt gtagctgcat ctcctgtcaa
    72601 tgtagaagaa taaaacacaa gtgcaactag cagaattgaa cctactaaat ttgcaaaata
    72661 aaccagtccc cagtttctta acatttttcc taatgttatt tttttgtcca taagtgcaat
    72721 agccattaag gtgtttccag tgaataattc tgctcctgcc accactacaa gcataagacc
    72781 tactggaaag accatagcac ttaaaaagct agctacccct gggtctatct tgccaaaagt
    72841 ttgactaact atcatactcc ccaatgcccc aaaacctata aatgctcctc caaataaacc
    72901 taatagaatt gcattttgtg tcgaaatgtt tgctttttta atgcctgcac ttatagttgc
    72961 ttgtgcagtt tccccaggtg tcaagaatcc tttactcata aatccttccc ccttaaaaat
    73021 tatactttcc cttgtctttt tatggcaata attatataat aacaatatga tatataataa
    73081 attttattaa cataaaaaat a
//
LOCUS       DAMJVX010000003        65413 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMJVX010000003 DAMJVX010000000
VERSION     DAMJVX010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 65413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65413
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.003"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            213..677
                     /locus_tag="PC407_000143"
     CDS             213..677
                     /locus_tag="PC407_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDF2495627.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            684..1178
                     /locus_tag="PC407_000144"
     CDS             684..1178
                     /locus_tag="PC407_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDF2495628.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1194..2219
                     /locus_tag="PC407_000145"
     CDS             1194..2219
                     /locus_tag="PC407_000145"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDF2495629.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2206..4653
                     /locus_tag="PC407_000146"
     CDS             2206..4653
                     /locus_tag="PC407_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDF2495630.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4833..6206
                     /gene="radA"
                     /locus_tag="PC407_000147"
     CDS             4833..6206
                     /gene="radA"
                     /locus_tag="PC407_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDF2495631.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDENIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6209..7279
                     /gene="disA"
                     /locus_tag="PC407_000148"
     CDS             6209..7279
                     /gene="disA"
                     /locus_tag="PC407_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HDF2495632.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7368..8462
                     /locus_tag="PC407_000149"
     CDS             7368..8462
                     /locus_tag="PC407_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDF2495633.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8591..10768
                     /locus_tag="PC407_000150"
     CDS             8591..10768
                     /locus_tag="PC407_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495634.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDMSTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSENGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVVFVELGDTYRLDLYKPNLNEKTYESVKDNIEKNIDVYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVLFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(10954..11799)
                     /locus_tag="PC407_000151"
     CDS             complement(10954..11799)
                     /locus_tag="PC407_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2495635.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCTYQDTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSGKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            12016..13182
                     /locus_tag="PC407_000152"
     CDS             12016..13182
                     /locus_tag="PC407_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HDF2495636.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNIVLDTHQYLMLAESDGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            13204..14850
                     /locus_tag="PC407_000153"
     CDS             13204..14850
                     /locus_tag="PC407_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDF2495637.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYIHSSGFDASILHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWRPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNVLGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIADESKDVD"
     gene            14947..16320
                     /locus_tag="PC407_000154"
     CDS             14947..16320
                     /locus_tag="PC407_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2495638.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNYIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMFFYKLDKEYSTI
                     LNELNTK"
     gene            16765..17718
                     /locus_tag="PC407_000155"
     CDS             16765..17718
                     /locus_tag="PC407_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDF2495639.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            17756..18718
                     /locus_tag="PC407_000156"
     CDS             17756..18718
                     /locus_tag="PC407_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDF2495640.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            18743..19729
                     /locus_tag="PC407_000157"
     CDS             18743..19729
                     /locus_tag="PC407_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDF2495641.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            19742..20788
                     /locus_tag="PC407_000158"
     CDS             19742..20788
                     /locus_tag="PC407_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDF2495642.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYSGKIRKCDVEKLTAKETIVSNNTSKYSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            20955..22685
                     /gene="lpdA"
                     /locus_tag="PC407_000159"
     CDS             20955..22685
                     /gene="lpdA"
                     /locus_tag="PC407_000159"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2495643.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEVPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            complement(23113..24330)
                     /locus_tag="PC407_000160"
     CDS             complement(23113..24330)
                     /locus_tag="PC407_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2495644.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(24412..24615)
                     /locus_tag="PC407_000161"
     CDS             complement(24412..24615)
                     /locus_tag="PC407_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2495645.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            complement(25076..25306)
                     /locus_tag="PC407_000162"
     CDS             complement(25076..25306)
                     /locus_tag="PC407_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2495646.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            complement(25303..25725)
                     /locus_tag="PC407_000163"
     CDS             complement(25303..25725)
                     /locus_tag="PC407_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2495647.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            26230..26583
                     /locus_tag="PC407_000164"
     CDS             26230..26583
                     /locus_tag="PC407_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2495648.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            complement(26643..26810)
                     /locus_tag="PC407_000165"
     CDS             complement(26643..26810)
                     /locus_tag="PC407_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDF2495649.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(26929..28848)
                     /gene="tet(M)"
                     /locus_tag="PC407_000166"
     CDS             complement(26929..28848)
                     /gene="tet(M)"
                     /locus_tag="PC407_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDF2495650.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            complement(28864..28980)
                     /locus_tag="PC407_000167"
     CDS             complement(28864..28980)
                     /locus_tag="PC407_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDF2495651.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(29225..30157)
                     /locus_tag="PC407_000168"
     CDS             complement(29225..30157)
                     /locus_tag="PC407_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2495652.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            complement(30154..31155)
                     /locus_tag="PC407_000169"
     CDS             complement(30154..31155)
                     /locus_tag="PC407_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDF2495653.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            complement(31152..33329)
                     /locus_tag="PC407_000170"
     CDS             complement(31152..33329)
                     /locus_tag="PC407_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2495654.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            complement(33332..35779)
                     /locus_tag="PC407_000171"
     CDS             complement(33332..35779)
                     /locus_tag="PC407_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2495655.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            complement(35763..36269)
                     /locus_tag="PC407_000172"
     CDS             complement(35763..36269)
                     /locus_tag="PC407_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2495656.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            complement(36244..36741)
                     /locus_tag="PC407_000173"
     CDS             complement(36244..36741)
                     /locus_tag="PC407_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2495657.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            complement(36858..37079)
                     /locus_tag="PC407_000174"
     CDS             complement(36858..37079)
                     /locus_tag="PC407_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495658.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            complement(37122..38327)
                     /locus_tag="PC407_000175"
     CDS             complement(37122..38327)
                     /locus_tag="PC407_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDF2495659.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(38350..38502)
                     /locus_tag="PC407_000176"
     CDS             complement(38350..38502)
                     /locus_tag="PC407_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2495660.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            complement(38505..39890)
                     /locus_tag="PC407_000177"
     CDS             complement(38505..39890)
                     /locus_tag="PC407_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDF2495661.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(39919..40305)
                     /locus_tag="PC407_000178"
     CDS             complement(39919..40305)
                     /locus_tag="PC407_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2495662.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(40321..40635)
                     /locus_tag="PC407_000179"
     CDS             complement(40321..40635)
                     /locus_tag="PC407_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2495663.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            41142..44090
                     /locus_tag="PC407_000180"
     CDS             41142..44090
                     /locus_tag="PC407_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2495664.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            44068..44532
                     /locus_tag="PC407_000181"
     CDS             44068..44532
                     /locus_tag="PC407_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2495665.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            44645..44947
                     /locus_tag="PC407_000182"
     CDS             44645..44947
                     /locus_tag="PC407_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2495666.1"
                     /translation="MGVTNVTKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            45009..46373
                     /locus_tag="PC407_000183"
     CDS             45009..46373
                     /locus_tag="PC407_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2495667.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            46408..46881
                     /locus_tag="PC407_000184"
     CDS             46408..46881
                     /locus_tag="PC407_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495668.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYINFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGDLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLRFRNKKFENKNI"
     gene            47053..47703
                     /locus_tag="PC407_000185"
     CDS             47053..47703
                     /locus_tag="PC407_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2495669.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTFKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            47877..48809
                     /locus_tag="PC407_000186"
     CDS             47877..48809
                     /locus_tag="PC407_000186"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2495670.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEDSIFIG
                     KNQNPYQEVHHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYRKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            49083..49796
                     /gene="ispD"
                     /locus_tag="PC407_000187"
     CDS             49083..49796
                     /gene="ispD"
                     /locus_tag="PC407_000187"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDF2495671.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            49793..50278
                     /locus_tag="PC407_000188"
     CDS             49793..50278
                     /locus_tag="PC407_000188"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDF2495672.1"
                     /translation="MRIGLGYDVHKLTENRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    50373..50581
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            50645..52360
                     /locus_tag="PC407_000189"
     CDS             50645..52360
                     /locus_tag="PC407_000189"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2495673.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDQCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            52482..53927
                     /locus_tag="PC407_000190"
     CDS             52482..53927
                     /locus_tag="PC407_000190"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2495674.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKTKGVAVEVDDRDNYT
                     TGWKFNEWEMKGAPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            54367..55848
                     /locus_tag="PC407_000191"
     CDS             54367..55848
                     /locus_tag="PC407_000191"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDF2495675.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKTKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLVEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEITEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            56069..56224
                     /locus_tag="PC407_000192"
     CDS             56069..56224
                     /locus_tag="PC407_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495676.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            56369..57766
                     /locus_tag="PC407_000193"
     CDS             56369..57766
                     /locus_tag="PC407_000193"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDF2495677.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKIFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            57751..58164
                     /locus_tag="PC407_000194"
     CDS             57751..58164
                     /locus_tag="PC407_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDF2495678.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            58271..58366
                     /locus_tag="PC407_000195"
                     /pseudo
     CDS             58271..58366
                     /locus_tag="PC407_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase (FAD)"
     gene            58672..59502
                     /gene="thyA"
                     /locus_tag="PC407_000196"
     CDS             58672..59502
                     /gene="thyA"
                     /locus_tag="PC407_000196"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDF2495679.1"
                     /translation="MSLADKIFIDMCNNILENGVSSDGEVVRAKWDDGQPAHTIKKFC
                     VVNRYDLSKEFPILTLRPTNLKAAIDELLWIWQKKSNNINDLNSRIWDSWADETGSIG
                     KAYGYQIGQKHKYKEGEFDQIDRVIYDLKYNPYSRRIITNIYNHNDLNEMNLYPCAYS
                     MTFNVTGNRLNAILNQRSNDILVANNWNVTQYAILVHMLAQISDLEVGELVHVIADAH
                     IYDRHIPLVKELINRESYDAPKLIINPNIKNFYDFRVEDFTLENYKSGPQIKKIPVAI
                     "
     gene            59515..60009
                     /locus_tag="PC407_000197"
     CDS             59515..60009
                     /locus_tag="PC407_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDF2495680.1"
                     /translation="MKVIVAVDLNWGIGYGGKLLQSIPEDMKFFKEKTVGNVVVMGRE
                     TFKSLPGKKALKDRVNIVLTRSKSFNDDRLIVCNSIDETLKELQKYENDKIFIIGGET
                     VYKQFLPYCNELYVTKIQKTYEADRFFPNIDIMSNWDLIKESEVKEYNNIRYVFSTYI
                     NNSL"
     gene            60036..61412
                     /locus_tag="PC407_000198"
     CDS             60036..61412
                     /locus_tag="PC407_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF2495681.1"
                     /translation="MKKDIFVNRVSNALENDRGAVFVGSGISFSSTEVDWFKLLEPLV
                     NELDITLDNKSDDLPMIAQYIVNNYSGNRGPLVNEISKTFNRKFKLNKYHKYLATTKL
                     STIWTTNYDMLLEDAFYDFDTSVKVSDDSITRNRLEYDVEIIKMHGCISRSDWNEIVI
                     TQEDYDDFSINKPAISQRLSEDLINKSFLFIGYSYRDPNIRNIMINARRLSKKHTQQH
                     YIILKKLKDSDSNITQDDVVRQELWCNDLKRLGISTLFIDEYSELEELLREISQKSRG
                     KTVYVTGSHKESNMNARKLGTKLAKHKDITLLSGQSSGIGSEVVAAFTEECIKSKEDI
                     INRMKIFPNPYSADEKYSDDITLIPDLKKCRTKMLNSTQVVVVFSGGIGTEAEIDVAI
                     KQNCNIIPVITCKEDRENPAIKKILNYSDNMKNIEIKDATYYNKIVDEDGVVTMEEIY
                     ICISKIMN"
     gene            61388..62488
                     /locus_tag="PC407_000199"
     CDS             61388..62488
                     /locus_tag="PC407_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDF2495682.1"
                     /translation="MHIKNNELKHDIIKLKKRNGFIEVGKDKKTLVNCLIGANELEDY
                     KYEIKKIDCILNISEKPDIVSDLSTKRRKISESIWYKIVKDTPFVSATLPIYLVSKNN
                     NKIDSNELLDIIIEQMENGVGMITIHPTANKQIFEDSKNRMVPVTSRGGGMVIQDLIS
                     KNFSEDNVYLKILPEIIKYAQKHNVCISLGATFRSGNIFDSNDRAQITEIKSQIELAK
                     RIYKSNVGVIIESPGHSRPSDIIKISSILKKEGFPIMPLGPIPTDIAIGMDHIAAAIG
                     ATIMGIEGCANIISTVTREEHTGGRPSIESTVESIRTAKIAAHIIDIHQMNDINLDMS
                     VSKSRAKSNTCIFGKDTRYCDRCRELCPLCIR"
     gene            62531..62956
                     /locus_tag="PC407_000200"
     CDS             62531..62956
                     /locus_tag="PC407_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HDF2495683.1"
                     /translation="MHRIASFSQQSQRYVKLEQFEYIIPPEIEKIEKAKELFINSMKK
                     YQEDYDKLVEILFENHYNKLIKGGKNEKTSKRQVEKKAIEDARYVFPNACETKMVFTI
                     NARSLFNFFEHRCYENGSCLEGTMTCGDIVQVRKKFKNL"
     gene            62969..63706
                     /gene="rlmB"
                     /locus_tag="PC407_000201"
     CDS             62969..63706
                     /gene="rlmB"
                     /locus_tag="PC407_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDF2495684.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            63709..64236
                     /locus_tag="PC407_000202"
     CDS             63709..64236
                     /locus_tag="PC407_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDF2495685.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            64301..64948
                     /gene="sigH"
                     /locus_tag="PC407_000203"
     CDS             64301..64948
                     /gene="sigH"
                     /locus_tag="PC407_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HDF2495686.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            65022..>65413
                     /locus_tag="PC407_000204"
     CDS             65022..>65413
                     /locus_tag="PC407_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF2495687.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVP"
BASE COUNT        24556 a         8670 c        12100 g        20087 t
ORIGIN      
        1 agttctgaaa tctaatcatg aagcaaaaaa ttagcatttg gttatcaata aatataataa
       61 ataaaaactc ataattaatg aaagaataaa ttcatatcta aatttctata aaaaatttaa
      121 atttatacaa tttagggctt taaaaattta aataataggt gtaaaataat agtcaaagaa
      181 agtcaaaaat aaaaaggtgt gtggtgatat taatggcaac tatgacagat ataattgaaa
      241 aatttattaa agatttgatg gaagaagata atagcataca aattcaaaga aatgaactag
      301 caaacttgtt tagttgtgca ccatcacaaa taaattatgt cttaacaaca agatttacaa
      361 tagatagagg atattacatt gaaagtaaaa aaggtggcgg aggatatgtt caaatagaaa
      421 aaatacgaaa aagtaaagat ggacatatta gagaactact aaatgaaaaa ataggaagtc
      481 agatatccta taaaaaagca aaggaattat tagaaggttt aaaagagtcg gatttgatta
      541 acgaaagaga attaaaatta atattatatg caatagatga taaatcattg tgtatgccaa
      601 tatatgaatt aaaagagaag gtaaggtcta atattttaaa gaatataata ataggattat
      661 ttagtataga ggagtgataa tttatgctat gtcaaaaatg caataagaat aaagcatctg
      721 tatattataa taagatagtc aatggagaaa aaacagagat gtatttatgt agtgagtgtg
      781 caaaagagaa tacagaaatg aattttaact tagatatgcc tttttcaatg atggatatat
      841 tttctaattt aggttttcaa cctaaaaaag aacttgagga aaagctagtt tgtcctaaat
      901 gcaatacaac atatagtgag tttaagaata atgggagatt tggatgtagt gaatgttata
      961 atgcatttag tagtcaagta aatccaatgc ttcaaaatat acatgggcat atagaacata
     1021 caggaaaagc acctaaaaaa tctttttata agataagtgt agaaaatgag attaaagaac
     1081 taaaagaaga tttagataga gctataaaaa atgaagagta tgaattagca gctcaattta
     1141 gagataaaat taaatattta aaaggttcta ttgactaaga ttggagtgat attatgaaag
     1201 agaacatagt catgaaatca agagttaggc tagctagaaa tttaaataac tatccatttc
     1261 caaataaatt agataaagaa tgtgccatgg aaataataga aaaagtaaaa aatgcgttta
     1321 ttaattctaa cttagaacaa aaagaagaat ttgatttcta taaaattgaa gatttagacc
     1381 aatctaaaaa aatgcttatg gttgaagaac atataataag cccagattta gcagaaaatg
     1441 ataaaagtgc agttatagta aaaaaagata aaactataag cataatgata aatgaagaag
     1501 accatataag gatacaaact atatgtgatg atttaaactt agaatatgca tatagtgtag
     1561 caaatgaaat agatgatttg ttagaatcaa gtttagaata tgcctttaac acaaagctag
     1621 gttatctaac atcttgtcca actaatacag gaacaggaat gagagcttct gtaatgatgc
     1681 atttaccagc attaagtcaa ctaggctata tggatgaact ttataaaatt tcatcacaaa
     1741 taggtatagc aattagaggg atatatggag aacgaacaga agcattggga aatatatatc
     1801 aaatatctaa tcaacttaca cttggaagaa cagagtctaa tattatagaa aatgtaagtg
     1861 gacttacaaa agatgcaatt tcaaaagaaa taaaagctag agaaatattg caaaaaaaat
     1921 taggtataaa actagaagat aaaatattta gatcgatagg gacacttgaa aattcaagag
     1981 taatgagtag cgcagaggca atgagccatc tatcaaatat aaaaatggga atagaaatga
     2041 actatataga taaactagat ttaagggcta tagaacaact tatgataggt atacaaccag
     2101 cgcatcagtc aataatgtat aagagtgatg atgttgagaa tagagatata aatagagcaa
     2161 catatattag agaaactttg gaaaaattaa gaggagggat gaattgtgaa cttcaataga
     2221 tttacacaaa gagctaaaaa ggcaattgat ttagcgtttg aatctgctaa aagtctagga
     2281 cacaatattg ttggaagtga acatatactt ttaggtcttt taagagaaga agaaggtata
     2341 gctgctaaag ttctaagtaa agttggattt acagaagctt atttagaagg caaaatagtt
     2401 gatatggaag gtaagggaga agaaatctca gaagatatag tattaagtcc aaggagtaaa
     2461 caaatacttg aattatcagg aatgtttgca aataaattaa aaacaaatta tattgggact
     2521 gagcatatat tattagcaat tattcaagaa ggtgaaggaa tagctaataa gattttaaat
     2581 tatgctggag taaatgatag gactttagcc caattaacaa ttgatatgat gggtattagt
     2641 gataaaaatc aatacaaagc agagaatagt tacacgggca accagaatca agcagaatct
     2701 aaagtacttg ataagtatgg aagaaatctt acactatatg caaaacagaa taaaatagac
     2761 cctgtaatag gtagagaaaa agaaatacaa agagtaatac aaatattaag tagaagaact
     2821 aaaaacaatc cagtgttgat aggagaccca ggtgtaggga aaacagccat agctgaaggt
     2881 ctggctacaa atatagcctt aggaaatgtt ccagaaacac taaaaaacaa aactttatac
     2941 tctttagaga tgggttcatt attggctggg gctaagtata gaggagaatt tgaagaaaga
     3001 attaaagaag ttgtagatga agttgttaaa aatggaaaca taatcttatt tatagatgag
     3061 atgcacacaa taataggggc tgggtctact ggagaaggct ctatagatgc ttcaaatata
     3121 ttaaagccag ccttggcaag aggagaaata caagttatag gtgcaactac aattgatgaa
     3181 tatagaaagc atgttgaaaa agattctgct ctagaaagaa gattccaacc agttatggta
     3241 gatgaaccaa ctaaagagga ctcaataaaa atattagaag gattaagaga taaatatgaa
     3301 gctcatcata aagttaaaat aactgatgat gctataaagg cagctgtaga attatcaact
     3361 agatatatat cagacagata tctgccagat aaagctatag acttgattga tgaagctgca
     3421 tcaaaagtaa gattaaaaga aaatacacct ccttcagaaa taaaaaaatt agaactagaa
     3481 atagaaaata tagataaaga aaaagaagaa gcagtaagat gtcaagactt tgagaaagca
     3541 gccaaaataa gagatgaaca aggtctactt aaaaaacaat tagaagatgt tagagaaaga
     3601 tggaacaagt catctaaaca ttcagattta gttgatggag aagttattgc tgaagtagtg
     3661 ggcttatgga caggaatacc agttaataaa attcttgaag aagaggctga tagacttttg
     3721 agacttgagg aaatactgca caatagagtt ataggtcaag aacaagcagt aaaatctatt
     3781 tcaaaggcaa tcagaaggtc aagagcaggt cttaaagacc ctaatagacc aattggttca
     3841 tttttatttt taggtccaac aggagtaggt aaaacagaat tatctaaggc attagcagaa
     3901 gtgcaatttg gagatgaaaa tcaaataatt agaatagata tgtctgaata tatggaaaaa
     3961 catgctgtgt caagaatgat aggttcacct ccaggttatg taggtcatga tgaaggtggt
     4021 caattaactg aaaaagtaag aagaaatcca tattcagtta tcttatttga tgagatagaa
     4081 aaagctcatc cagatgtatt taatattctg ttacaaattc tagatgatgg tagactaact
     4141 gattcaaaag gaagaactgt agattttaag aatactattg taataatgac atcaaatgtt
     4201 ggagcatcta caattggtag acaaaaaact ttaggattta gtatagctaa aggagacgaa
     4261 gaagaaaaat ctcaatatga aaaaatgaaa gaaaatataa tgggtgaatt aaaacaaaga
     4321 ttcagaccag agtttttaaa cagaatagat gatataattg ttttccactc actaaatgaa
     4381 aatcatatat caaaaatagt acttttaatg gcagcgaaac tacaagagag attaaaagaa
     4441 atggatataa aattagaaat gagtgatgag gctgttaaat taatttctaa atctggattt
     4501 gacttagaat atggagcaag acctcttaaa agagctttac aaaaagagtt ggaagatgag
     4561 ctatctgaag caatcttaaa aggtaatgtg aaaaagggaa gtaatgtagt agcaaaagtc
     4621 aaagatgaaa aaatagtttt tgaaactaaa taatttaata gagttgcatt ttaaaatatt
     4681 gcaaatataa gttttataat ctaaacaaaa ttttaagggc aatgaatcta taataattca
     4741 ttgtccttaa ttattttatt taatctttac atattgtctt tcagatatgc taaaattata
     4801 gtatagaata aaaaattaag gatgataaaa gtatggcaaa aataaaaaca aaatatgtat
     4861 gtcaatcttg tggatatgaa acagctaaat ggcttggtaa atgtccagaa tgtacaaaat
     4921 ggaatacatt tgtagaagaa atagaccaaa aaagcaccaa aaaagaggtc tttataatag
     4981 ataaatcttc atctaaacca gtcagtataa actcaataga aagtaaagaa gaagagaggt
     5041 ttacaacaga tataaatgaa ttagatagag tgcttggagg aggtatagtt aaaggctctt
     5101 tagtgcttgt tggtggtgac cctggtatag gtaaatcaac acttttaatc caagtatcta
     5161 gtaatgttgc caatttagga aaaactgttt tatatataac tggagaagaa tcagaatctc
     5221 aaataaaaat gagagctaaa agattaggta ttaactcaga aaatttatat atatttgctg
     5281 aaaataattt aagtataatt gaatcttatt tagaaagtgt aaatccagaa ttaataattc
     5341 tagactcaat tcaaactgtc ttcagtccag aaatatcctc ggcaccagga actgtcagcc
     5401 aaattaaaga aggtacttca aaattcatga aaatttctaa aaaaatggga atttcaacgt
     5461 tcatagttgg gcatgttaca aaagaaggtt cactagcagg accaaaatta ttagagcata
     5521 tggttgatac tgttctatat tttgaaggag aaagatataa tacttataga ttagtaagag
     5581 ctgttaaaaa tcgatttggt tcaactaatg aacttggagt atttgaaatg agggatttag
     5641 gattagtaga acttgataat ccatctaaaa ttttaatctc tgaaaaacca aaagatgtag
     5701 caggttcagt aataatatct actgtagaag gtacgagacc aatgttactt gaattgcaag
     5761 cattagtttc tcctacaagt tttggtatac caaaaagaac atctacagga gtcgattaca
     5821 atagagtagg tatgttatta gcagtattag aaaaacgtgt tggtttgcaa atacaaaatc
     5881 aagatgtcta tataaacata gttggaggaa taaagataaa tgaaccctca attgatttag
     5941 ggatagccat ttctgtagca tctagtttta gaaatatacc gatagatgaa aatatagctg
     6001 ttacaggaga agtgggactt acaggagaag ttagagcagt aagctttata gaaaagagaa
     6061 tagctgagtg taaaaaactt ggatttaaaa agatagtagt tcctagaagt aattatgatg
     6121 ttgtaaaaga gaccaaaggc atagaaatat ggccagtaga taacttaaga caagctataa
     6181 atatagtact tgggaggaat caataaatat ggagaatttt ctagataata aaaatatgct
     6241 atatgcatta aaaatgatat ctcctggaac tccacttaga ttaggtctaa acaatgtact
     6301 aagagctaag actggtggat taattgtaat tgcaacaaac gaagatgtaa tgaaaatagt
     6361 agatggagga tttgctataa atgcagaata ttcaccatca tatctatatg aattagctaa
     6421 aatggatgga gctatagttt taagtggtga tgtaaagaaa atattatttg ctaatgcaca
     6481 acttatacct gactatttta tagaaacatc agagacagga acaagacata gaacagcaga
     6541 aagagtagca aaacaaactg gtgctatagt cataggaatt tcacaaagaa gaaatgttat
     6601 aacagtttat agaggaaatg agaagtatgt agtcgaagat atatctaaga tatttactaa
     6661 ggcaaatcag gctatacaaa ctctggaaaa atataagaca gtattggacc aagctgtaac
     6721 aaatttaaat gccttagagt ttaatgattt ggtaactatt tatgatgttg cattagtcat
     6781 gcaaaagatg gaaatggtaa tgagagttac aagtataatt gaaaaatatg tgatagaatt
     6841 gggtgatgaa ggaactttag taagtatgca attagaagaa ttaatgggta caaccagaat
     6901 agaccagaaa ttaatattca aagattataa taaagaaaac acagaaataa aagaacttat
     6961 gaaaaaggtc aaaaatttaa attcagaaga actaatagaa ttggtcaata tggcaaaact
     7021 attagggtat agtggttttt cagaaagtat ggatatgcct ataaaaacaa gaggttatag
     7081 gattcttagc aaaatacata gactaccaac agcaataata gaaaacttag taaattattt
     7141 tgaaaacttt caacaaattt tagatgcatc tattgaagaa ttagatgagg ttgaaggaat
     7201 aggtgaaata agagcaacat atataaaaaa tggactcata aaaatgaaac aattagtctt
     7261 attagataga cacatatgat gaattttaaa tgaattttac tacttttttc ttgaatataa
     7321 tatacacttt ggtctataat agataagtat aactaggggg tgaagcattg ataaggaaag
     7381 taacaagaat attgtttatt ttacttggat ttacaatagg tataacaacc tatttaacat
     7441 taatgaaaga ttttgaaata ttaacatttg gaaaagaaac atatggttat atcgcagctg
     7501 tagtagcagg tataataata gccattttag gatatcttat agaaccatgg gttgtaaata
     7561 aagtaaaaga aattgctaaa atagtggata aagaattgtc aaaatatcct cagactgata
     7621 tattgttagg gtctatgggt cttatagtag gatttgttat tgcgtattta ttaagtggat
     7681 tagtaaatag aataccaata gtaggtggaa tactatcctt actattatat ttattcctag
     7741 gatatcttgg aatgaaagtt gcgcttaaaa gtaaaaatga tttatttaat attggaaaac
     7801 taggaagatt agcaaatcct attaaggata aagataagga taaagaaaat aaaaaagaag
     7861 taaaagccat tcctccaaaa gtattagata caagtgtaat aattgatgga agaatagctg
     7921 atatttgtaa gactgggttt atagaaggaa aacttattat tcctgctttt gttttggagg
     7981 aactaagaca tattgcagat tcttcagatg atttaaaaag agtaagaggt agaagaggtc
     8041 tggatatatt aaatataatt cagaaagaat taaatataga agttgaaata agtgagagag
     8101 actttgatga tatagcagaa gttgatagta aattattaaa gcttgctcag gttttaaatg
     8161 gaaaagttgt tacaaatgat tataacttaa ataaagttgc tcaattccaa ggtgttgaag
     8221 tattaaatat aaatgagtta gctaatgcta taaaaccagt tgctatacca ggtgaagata
     8281 tggttgttca agtggttaaa gaaggaaaag aggctcaaca aggtgttgca tatctagatg
     8341 atggaactat gatagttgtt gatggaggaa gaaaatatat gaatgagact ataaaagtac
     8401 tcgttacttc tgttcttcaa actcctgcag gtagaatgat atttgcaaaa cctaaaaatt
     8461 aatatgttca aagaaaccta taatttgggc tgtttatttt aaatagatgg tccaaattta
     8521 tttatttata acgataatgt ttaatgtatt cttaaagatg gggatttaaa tcagaaagca
     8581 gggagaattt atgagaaaaa agattatatc tttcttagca acatttatca taatattaac
     8641 atcagcaagt cagtatagtt ttgcagatga tatgtcaaca cgtggaaaag taatatttat
     8701 agatatgaat agaactagta tgagtaatat gttaagaata aaatcattaa gagaagaact
     8761 agacaataga gggtatattg gcttgatgaa tataagaggc gataaaggaa gtgatgatag
     8821 aaggtcatat gctagtatgg gtgctggtgg tagagctaat gtggctaatg aagaagatat
     8881 taattttgaa agctcgagta aggatagaaa tatagttttt gaatcagcta caggaaaaag
     8941 tgcaaaaggt ataaataatt taactataaa taagtctata aatgaaaatt taaactttgg
     9001 agagtatggt tctgtattag ggtcattagg acaaagttta tctgagaatg gattaaaagc
     9061 atctgtattg ggtaattcag atataattga aaatggtcaa cttataaaaa atagaaactt
     9121 atgtctaact gcaatggatg aatatggaag aatacctaat ggaaatgtag atactataaa
     9181 taaaaaagac ttatctatgc cttatggaat atcaactgat tatgataaat tgattgtaga
     9241 gaccaaagaa gcttacaaaa ataatgatgt ggtttttgtg gagcttggag acacatatag
     9301 attagattta tataaaccaa atttaaatga aaaaacatat gagagtgtga aagataacat
     9361 agaaaaaaat atagatgtat atttaaaaaa tatattttct atggttgaag aaaatgacac
     9421 tgtctacata gcaagtgcat ttccaagtga tttagattat aaaaataaaa gaagactttc
     9481 tccaataata aaattaaatg gagaagggaa aggtatatta tcatcttcaa ctactagaag
     9541 agaaggtatt gtaacaaatt tagatgttgg ggttgaaata ttagacaact ttaatataaa
     9601 aaatcaaaat atggttggaa gaaaatatga acttattaac agagatgata ataaagaatt
     9661 tttgatggat gaatatcaaa agatagtatc tatttcaagc ataaggtcta caatactgaa
     9721 tggttttgta agtatagttt tcttgtcatg gatagttgct atgattgcaa ttttatttag
     9781 aaagcatata tcaaaaaatt ataaagaaac aacatttttt atattaaagg aacttttaaa
     9841 aataggaata gtaatgccac tgtcatttat gataacacca ataatgaatt tcaaaacacc
     9901 attagcaata tctttaggaa taattataat aacacttact atatatctta taagtaaagt
     9961 tttaataaag aatgatttaa aaaacatgct gttttttact ggattaacca tagtaattat
    10021 ggttattgat gctgggtttg gctcatattt aatgaaaagc aatgttatga gttatgactg
    10081 tataattgga gctagatatt atggagtggg aaatgagtat caaggtgtgg caatcggtag
    10141 tgcaatattt acatttgcaa ttttacttac atataaaaat ataccaaagt ggtctataat
    10201 agttttttct ttggttatct taattacaag tgcatctcca attatgggag caaatgttgg
    10261 aagtgctatt tctgaatgtg ttgcattttt actatttata ctattgatat ataatgtgaa
    10321 gatagacttc aaaaaaattg tattattagg tatagcagta ttatttgtat taggagtttt
    10381 tgtagcaata gatatgattt tgggaagtaa ttcacattta ggtatgtttg taaaagaaat
    10441 ttactttaat ggaccaggag aaataataca aacatttagt agaaagatag agatgaattt
    10501 aaagctagct caaaccagtg catgggtaaa tatactgtta actggaatag gtgtaatttt
    10561 agttcttatg attaatcaga ttagatactt taagcaactg atggatgagt atccaattgt
    10621 atttaaagga tttatagcat caatagcagg atgtttagta actcttttag tgaatgactc
    10681 tgggattgtg tcatcagcta cagcctttat ttatgtaatt gtacctatga taacattaag
    10741 tataaatctc acagcattaa aagaataagt actgtatgaa taatatgtaa atatgaacac
    10801 attaatttaa cttctttatt tacaaaataa agaagaaagt ttataaaaaa taaattataa
    10861 taaaaaagag tttacctcaa aattgattat tctaatcaaa ttttaaggta aacttttttt
    10921 attataaaga taaataacat ataacctcat attttaggta gatttttttc ttatatgttt
    10981 aggagtgcat ttaaattttt ccttaaaaat tctgtaaaat aattttgtat tagtaaatcc
    11041 atgagcttct aatatactgc ataaggtatc atttgtgtta attaagtctt tataaatatg
    11101 agaaatacgc acatcattta aatactctaa gtaagtttgt cccatatact gtttaaattt
    11161 tctacaaaaa tattctggct gtaagtgtgc taccttagcg attttgttaa tagaaatagg
    11221 cattttgtaa ttaatctttg tatattctaa aactggttca aattttgaca aatcaagtac
    11281 tgtttttttc atatttttat tacctacaga tattttaaag tttgtatata attcaaataa
    11341 aagttcaaat aataagcttg taaagagcag attagctgca ggaggagagc taagtttaat
    11401 ttctctcatg ttttctaata taagtttaac tttacctgaa ttttttttat agttattgtc
    11461 attggcatta accttaaagt caaaatagca atttttaaaa tcagggatat atttgtttag
    11521 aaaagacaat ggtacttgca ataatataga ggtatcttga taagtacatc tagttgagtg
    11581 aataattcca gaattaatga acatacactc attagctttt aaattataag tgtaaccctc
    11641 catgttaatt tgtaaatcac cagaagttat ataaattatt tctaatgagt tatgccaatg
    11701 atttgctaca taactaccac tatcatcaaa tagtttgaaa ttaacatcta aattatcaat
    11761 aggttcaatt atttcatgtg aaaaattatt tctgttcata ataatatcct ccttataatt
    11821 ttaaattata aatagtaaat ttataatgtt caatattaaa aagataatat agactaataa
    11881 attagataat aaacatactt aagaaaacgg ataaaatcaa atataataac tgtatattaa
    11941 aataacttaa aaaaattatt ttattataag tgcaaaatat acctatttaa ataagataag
    12001 aggggaggat tttatgtgta tataaaaggt gtaaatttag gaggttggtt agttttagaa
    12061 aaatggatga cctcaagttt atttgaaggc acagaagctg aagatgaata ctatttacca
    12121 agacagcttt caagagaggc atatgaatct agaattaaga ctcatcgtag tgaatacatc
    12181 acagagagag attttgcaac gattaaatct atgggattta attcagttcg tataccagta
    12241 ccatacttta tttttggaga ttgtgaacca ttcataggat gtgttaaaga acttgataaa
    12301 gcatttgctt gggcagataa gtatgggctt agtatattga tagacttaca tacagttcca
    12361 ggaagccaaa atggatttga taatggagga atatcaggta tatgtagctg gtcacaaaat
    12421 ccagaatatg tagcgtttac attaaatgta ttagaaagac ttgcaaaacg ttatgggatg
    12481 agacatgaat tgtatggaat ccaaatttta aatgagccta taacagaaag aatgtggaac
    12541 ataatgaatg ttccaaatcg gtttaaggct gtagataagg agatggcaag ggggagtaaa
    12601 cctaattcat tagagttttt acgtgatttt tatataaaag catataaggt aatgcgtcct
    12661 tatatgagag aggaaaacgt tatagtgttt catgatgcat ttgaattaaa agcatggaaa
    12721 gactttatga gagaggaaga atttaaaaat attgttctag atacacacca atatttgatg
    12781 ttagctgaat cagatggatg tgaacaaagt attgatagct atctcaaata tattagagaa
    12841 aattatgcta aagatatttt acaaatgcaa aaatattttc ctgttatatg tggggagtgg
    12901 agcttattta attcatatgc ttgtggtata gatacaaatg gaggtcaatc acctctaaat
    12961 gggattgaat caaatataga taaattatca aaagacgata aaagagaatt atataggaag
    13021 atagcaaaag cacagcttga tgcatggaga aatggaagtg gtcactacta ttggaattat
    13081 aagttattac tagatacagt taatgaagaa ggttggatag gatgggatag ttgggaccta
    13141 ggcaaatgtg tagctcaaga atggtatcct atagaatatt aaactaaaat tggaggaaaa
    13201 gatatgatta aaaaccctat acttccaggt tttaatccag atccttgtat ttgtagaaag
    13261 ggagatgatt attatcttgt agtttcatct tttgaatggt ttccaggtat acctgtttat
    13321 cactctaaag acttaaaaaa ttgggagtta tatactcata ttttgacaga tgaaacaaaa
    13381 atagatttga agaagcttcc ttcatctaaa ggaatttggg caccttgttt gacttattgt
    13441 gaagaagaag atttatttta catagtttat ggaattatga actctatgaa tgcaagatat
    13501 tttgatgttg ataattattt aattacatca aaagatataa agggagagtg gagtgaacca
    13561 gtatatatac actcatcagg ttttgatgcg tctattcttc atgatgatga tggtaaaaag
    13621 tggattgcat cattagactg ggagactaga gaaggatatg aaaaacctgg tgttatatgt
    13681 ttagtggaat actgtacaaa aaagaaagaa atagtaggat atccaaagag aatatggtca
    13741 gggggaactg atagaggttg tattgaagca ccacatataa caaagcgtgg agattactat
    13801 tatataatgt gtgcagaagg tggaactgga tatggtcata gtgtaactat gggaagggct
    13861 aaaaatatat ggagacctta tgaaaaagat tcaatgaatc caattgtaac ttcaattcca
    13921 ggcgattttt atgaaagaca tgaccctgac catctaaagc ctaaatacta taatccagaa
    13981 tcaaaacttc aaaaatctgg tcatggaagc tatatagaaa caacatcagg tgaagtatat
    14041 ttagttcatc taacatctag accatttgta ccagagttaa ggtgtacttt aggtagagaa
    14101 acagctattc aaaaaatgaa atggacaaag gataattggc ttcgaatgga agatgaaagt
    14161 aatttggcaa aagaatacgt atcagaaagt aaactagaag aacatttagt ttcatctata
    14221 ccaagttttg atgactttga ttcaaatgta ttaggtttac agtactatgc tcctaggatt
    14281 tcaccattat catttgcaga tgttaagtct agaccaggat atgtaaggat tagaggtcaa
    14341 gaatcaagaa catcattaaa taaagtaagc atattggcaa gaaaattaac ttcagtatat
    14401 gcaagaataa ccactaagat ggagttttac ccagaggtac atcagcatag tgcgggtttg
    14461 ataatgtatt atgataatat gaattatata aatttaagaa agtattatag tgaaacattg
    14521 ggtcaaagtg cactatcaat aatacatctt gaaaatggag agaagacgga atttttaaat
    14581 actaggatac caataaaaga tataccaata tatcttcgtc tgtatataca aggtcgtaag
    14641 tcatattttg aatggagtta tgatgaaaaa aactaccaaa gaataggaaa ggtatttgat
    14701 acaactaagt tttcagatga gtactgtaaa tatggagaat ttacaggtac gtttatagga
    14761 ttaacttgtg cagatcgtgt aaagcataaa cactatgctg attttgattt ttttgaatat
    14821 atagcagatg aatcaaaaga tgtcgattaa taaaatcatt actaaaattt tcagagcaaa
    14881 ggtaaatctt atgctctgaa ataagaaaca ttagaaggca aatcttacaa aaaggagtat
    14941 ggtattatga atacagaagt taatgttaaa gcaactacga ttagatatga aaagatttca
    15001 ttttttgaaa gaatttctta tggttgtggg gatttggggt gcaatatcat atactcagct
    15061 atgtcagcat ttttgctgtt ttattatact aattatgcag atgttagtgc agcagctgta
    15121 ggtagtatta tgcttgtatc taggatactg gatgggttta gtgacttaac aatgggaatt
    15181 atagtggata gaactaaatc caaatacggt aaggctaggc cttggatttt gagaatggcg
    15241 attccttttg ctatagcagc agtattatta ttttctgttc catcaaatct tggtatcact
    15301 tctaaattaa tttatatttt tattacatat aacttagttt ctactgttat ttatacagca
    15361 atcaatgtac cctatgcaac tctaaattca cttataactc aaaaccagta tgaaagagga
    15421 gtattaagta tatttagaat gatattagcc acttgtggta ccttactaat aaatggactt
    15481 acactacctc tagttgaata ctttggaaat aatttatcag catggactaa aacattcttt
    15541 gtatttggaa tagcatctat aatggtattt tttataacat ttactggaac caaggaaaga
    15601 gtaaaagcag ttaagcaaga taggaatgaa gtaataccat ttaaaatagg tattaaatct
    15661 ttatttagaa ataagtactg gatacaaata acattatgtt tagtttgcat atttattgta
    15721 tttgcgataa atggtggttc atccgtatat tatgcaaagt ttattttggg agatgaaaaa
    15781 ttatttggtc ctataaatat ggtttcaaat atatcccaaa ttatagctat gtttatggtt
    15841 gctcctttta taaaaaaatt tggaaaaaga aatgtattaa tagtaggttc aataatatta
    15901 atatcgtcaa atgttatgtt tataatagtt ggacaaaact atataggagt aatatttgca
    15961 agtgctataa agggaatagg aagtgctgga atagcatcaa caatgtttgc tattgtttca
    16021 gatactattg aatatggaga atggaagact ggatatagaa ctgaggggct tataaatagt
    16081 gcatctagtt ttgggtttaa agtgggaaat ggattaggtt cagcaatatt aggtgcagta
    16141 ttatcaattg gtggttatgt aggaacatca gctactcaaa gtgatttagc tatagtatca
    16201 ataaaagtat gttttatata cctacctata tttataacaa tattacaaat tatcataatg
    16261 tttttttata agcttgataa agaatatagt actatactta atgaattaaa tactaaataa
    16321 tgaaaaagtt tcttataaag tcttaagttt aattaaggtc tattgtaaat gttccctata
    16381 aaaactatta tatatttatg attattgaag aatttttaga gaagcgaata ataaaaatat
    16441 aagaagactt taaatgatga aatggttggt cttttagaaa tgatttaata agttattttt
    16501 atttaaagca ttataagtgg acaattaatt tttaatttat ggagatgata tttaacaatt
    16561 agaagtaata tataatatgc gctaagaagc aatttttcaa ctatttatga aggtggtggt
    16621 acaagaaaag tatcaccttt tttttgttat acaaaaaaac agttgacttt ttatatgaac
    16681 cccttctata atgttactga aattaagtaa aatgattgaa caaatgtaaa gataattgaa
    16741 aagcttgatt ttggaggaaa tcaaatgaaa aaaattggag acttaagatt aatgatgaag
    16801 tgttgttcac tttattacga agataatttg aatcaacagg aaatagcaaa tcaattggga
    16861 atatcaagac caacaatatc aagaatatta aaagaagctt ttgaacaagg aatagtaaaa
    16921 atacagatag ttgatgtttt gaaaaatgat taccaaaaaa tagagagaag tttggagaga
    16981 aaatataaat taaaagaagt aatagtagta gatgataaac aagatgcact aacacaaaaa
    17041 caagaactag caagagctgt atctgaatat ttaactagag tagtaaagga aaatgatata
    17101 ataggtgttt caataggaac aacactaaaa gaaataccaa gatatgtgga aaaaagtaac
    17161 tgtaaaaatg ttacatttat acctctatta ggagggattg gagacaatga aatagatatt
    17221 catgcaaatc aaatagcagt aagtctagcg agggcttttg gaggtgattt taaattatta
    17281 catgctccag ctgttatgtc tgacttgtct acaaaagaga aactttgcaa agacgaaaaa
    17341 attaaagaag tactagattt aatagataaa acaactatag caattgtggg tataggaaat
    17401 cctatgagtt taaactcaac aataatggct tctggataca tgaatgaagc agatatagaa
    17461 gatttaaaaa aatacaactc aataggtgca atttgtttac aagcctttga taaagaagga
    17521 aaaacttcta tacttgaatt taatcaaaga gtattaggag tgaaactgga agatttaaaa
    17581 aagataaaaa gaacaatagg tgtcgcttca ggagatgaaa aaatagaggc aataaaggca
    17641 tcattaaaag cgaaatttat aaatagttta gcaattaatc acagtttagc acttaaatta
    17701 ttagaagatt gtgactaata gaaagataga taaaagttaa caggagggaa tgtatatgtc
    17761 aatatcaaaa gaaacattgc ttgaaatgta taagagaatg aatcaggcta gaaaatttga
    17821 agagaaagtt agttggtttt ttgcaagagg tatggttcat ggaacaactc acttatctgt
    17881 aggtcaagaa gcgtcaagtg tggcggctgt aatggcttta gaaaaaggag atttagtatc
    17941 attaactcat agaggacata gtcaatttat aggaatggga atagacctaa ataaaatgat
    18001 ggctgaactt atgggtaaag aaactggatt ttgtaaggga aaaggaggct caatgcatat
    18061 agcagatatt gagtcaggta acttaggagc aaatggagtt gtaggtggag gtcttacaat
    18121 agctccagga gctgcactta cacaacagta taaaaaaaca ggcaaaatag ttttatgtag
    18181 ttttggtgat ggagcatcaa atgaaggtac ttttcatgaa ggaataaatt tatcttcaat
    18241 atggaaattg ccaataatat tttactgtga gaataattta tacggtatgt caacaagcat
    18301 aaaacgtcat atgaatatag aaagtattgc cactagagca gcttcttatg gaatagaagg
    18361 aatatcaata gatggatata acccaataga agtatacgaa acagtacaaa aagctgctga
    18421 gaagtgtaga agaggagaag gacctgtatt aattgaaagt agaacttata gatggttagg
    18481 tcattcaaaa tcagatgcaa atgtatatag aacaaaagaa gaaatagaat catggaaagc
    18541 aaaagaccct attgaatttt taaaaaatta tcttatagaa aataatctat ctaatgaaga
    18601 tgaattagat aagattcaag aatttgcaaa gcaatcaata gaagatgcag tagagtttgc
    18661 tcaaaatagc ccaaatccaa aaatagagtc tttattagag gatgtatatg cagactaaga
    18721 atggtaaagg aggaaaataa aaatgagtac aagggaatta acatatgcac aagcaataaa
    18781 ggaagcaatg tcagaagaaa tgagaagaga tgaaaatgta atatttatgg gagaagatat
    18841 aggaatatat ggaggggcat ttggtgtatc agttggtatg atagatgaat ttggaccaga
    18901 aagagtaagg gatactccta tatcagaagc agctatagca ggagcggcag caggtgcagc
    18961 agcaactggt ttaagaccta ttatggaagt aatgtttatg gactttgtaa caatttctat
    19021 ggatgcaatt gtaaatcaag cggcaaaaat gagatatatg tttggaggaa aagcacaagt
    19081 tccaatggtt gttagatgtc caggtggttc tggaactggt tcagcagagc aacattcaca
    19141 aagtttagaa gcttggtttt gtcatgtacc aggagtaaaa gtagttgcac cttctacacc
    19201 agcagatgct aagggattgt tgaaagcagc tataagagat aataatccag tcatatttgt
    19261 tgagaataaa ttgttataca gaaaaaaagg ttttgtgcca gaagatgatt atgtaattga
    19321 aattggaaag gcagatatta aaagagaggg tactgatgtt acagtaatta cttatggaag
    19381 aatgttacaa agtgttgaag aagcagcaga gactctatca aaagaaaata taaatgttga
    19441 aattatagat ttaagaactt tatatccatt agataaagag actatagtaa agagtgtatg
    19501 taaaactggg agagttttaa tttgccatga agcagctaag actggtgggc taggtggaga
    19561 aatatcagcc ctgataacag aaagtgaatc atttgattat ttagatgctc cagtaaaaag
    19621 aatttgtgga aaagatgtgc caataccata taatccagag ttagaaaaag cagttgtccc
    19681 aagagttgat gaaatagaag aagcaataaa atctttaatt gttagataag gagggataga
    19741 tatggtggca aataaaataa aagccacacc agcagctaga agtcaggcaa gaaaagacaa
    19801 tataaaatta gataggctga ttggaagtgg agaaaatgga aggattcatt tagtagatgt
    19861 gttaaattat ttaaaagata ataaagcaaa tactactcct ttagctagaa gaatagcaga
    19921 agatttaaat atagatttag aaactattgt aggtactgga tatagtggaa agataagaaa
    19981 gtgtgatgtg gaaaaattaa ctgcaaaaga aactattgta tcaaataata catctaaata
    20041 tagtgagaaa aaagagctga aaattgaaaa tgaaaattca agcatgttta acacagtaga
    20101 aggaatattt gaaaagccaa atccaatgag agctactgta gcaaaacgaa tgtcagaaag
    20161 ttattttagt gctcctgtat ttacattcaa tatagaagta gatgctacag aattgaaggt
    20221 acttagagca aagttaatcg acacagtaaa agaatcaact ggagtaaaat taactatgac
    20281 agatttaata gtaatggcag tatctaaaat acttccaaat catcaagcct taaattcagc
    20341 atggacagat gagggtatat ttagatataa agatgtaaat atagctattg cagttggttt
    20401 agatgaaggc ttatatgttc cggtagtaaa aaatgcaaac aaaaagtctt taaaagaaat
    20461 agcaaaagaa agcaaagaac ttgcggaaaa agtaaagaca gggaaactta tgccagcaga
    20521 ccaagaaggt aatacattta caataagtaa tgttggaatg tatggtataa ctacatttac
    20581 acctataata aatatgcctt caagtgcaat cttaggggta ggtgctacac aagataagtt
    20641 tgttccagta aatggagagg ctaaaataaa acctataatg aacttatcat taacatcaga
    20701 ccatagagta atagatggaa cagtagctgc aaaattctta aaagatttaa aagaattgtt
    20761 agaaaatcct ttatcaatgc ttgtataggt tttgatattt cgttactgat ataaatagtt
    20821 tgaatagatt atattattat aattgtttca gtaaatttaa agcttttata tattaagaat
    20881 tatttttaac agtaactata tgtgaatatg atttgcttaa ttaaaatata tgaaaataaa
    20941 taggaggaga gcctatgtca gtagaagtaa taatgccaaa agcaggagta gccatggaag
    21001 aaggtactat agtatcttgg ctaaagcaag aaggcgaaga agtaaagatt ggagaaccta
    21061 tactagaaat aacaacagat aaagttaata tggagataga gtcagaagga gaaggtacac
    21121 ttgctgtaat catacataaa gaggaaggtg aagtattacc agtatttaca gtaataggtg
    21181 taatagcaga aaaaggggaa aatcaagagg aagtaaaagc taaatattta tctggaaatg
    21241 tttcaaaaga agatatagtt gaagagaatc aaaatataga agttaaagaa gaaaaaataa
    21301 acaaaaaaga gtgtaatcat gattatgatg tagtggtaat aggaggagga cctgggggat
    21361 atttatcagc attaaaagca gctcttttag gtggaagagt tgctttagtt gaagaaaata
    21421 tactaggagg tacttgctta aatagaggtt gtatccctac aaagacttat ataaaaacag
    21481 cagaaatatt agaagaaatt gaccaattat caaagagagg tgtaaaagta actgtagata
    21541 aagaacagga cattaagaag gccataaaat ataaaaatag agtagttaaa aaacttacag
    21601 ctggtgttgg cggattatta aaaagtagag atgtagatgt gtttaatctt aaagctagtg
    21661 ttaaagaaga gcataaagta attttatcag atggaaaagt tttagataca gaaaatataa
    21721 taattgctac aggttccaaa gtaaggatat tacctataaa aggaatagaa tcaaatttaa
    21781 taataactag tacagaagca ttagatttgg aaacagtgcc agaagaatta gtaataattg
    21841 gtggtggagt gattggttgt gaatttgcag agatattcaa ttcaagaggt tctaaagtaa
    21901 ctatagtaga aatggaagat agagtaatcc caagaatgga taaagagtta agcgaatctt
    21961 taaaatattc tcttagtaaa aaaggtataa atgtattaac taaaaagaaa gtttctgaat
    22021 ttaaagaaga aggaaataac atattagtgt gcattgaagg tgaagtgcca ataaaagcag
    22081 atttatgtct atatgctata ggaagagaag ctaatctttc tggcatagag gacttagata
    22141 ttaaaataga taaaggttca atagttgtaa atagtaaaat ggaaacaagt ataccaagca
    22201 tatatgcagt aggtgatgtt actggaggtg taatgttagc tcatgctgct tttaaaatgg
    22261 gagaggtagc agcttcaaat gctctaggtg tgaataaaga agttgactta ggtgcattac
    22321 ctagttgtgt ttatacaatt ccagaagtag catcagttgg tattacagaa gaggatgcta
    22381 gaaaaaaata taatgtaaaa gttggtaagt ttaattttgc tggaaatgga agagctttag
    22441 cttctggtca agaacaagga tatgtgaaag tagtagcaga tgctaaatat ggagaaattt
    22501 taggtataca tatgtttgga tgtggtgtag cagaacttat aaatcatgca gcatcattta
    22561 aagcattaga gatacctact gatgaagcaa gtgagttgat atttggtcat ccatgtactt
    22621 cagaagcttt aatggaagct ttggcagatg taaatggaga atgtttacac ttacctaaaa
    22681 aataaatcta aacataaatt attaatttaa gtctattatt aatattaatt tatatcataa
    22741 aattttaaaa cacttactta gccatacccc ttattatgca aataatttat ttgtataata
    22801 aggggtattt tttatggaaa aatatagaat atagaatcct tgtagttttt gttgtacaaa
    22861 aaagatagta atataaaata acattatcat tttagaatga tttcaattgt aattaggaaa
    22921 agtgattttt ttgtgtaaaa acaaagtata aatttctaat tatcttttta tattttctta
    22981 aatgctcgta aagccttatt ctatgtgctt tcgagtattt ttactgtagg aagatacttc
    23041 acgtttcttt gcatatttcc tcatgtctta gctgtcagaa gtggtaaata agtagtaaat
    23101 tcatttgtac tactaagcaa caagacgctc ctgttgcttc tctttattca agcgtttcat
    23161 ttctgccatt gcagaatcga atgttgcatg tgcgtaatag ttcagcgtca tggctatatt
    23221 agcatgtccc ataatgtact gtaatgcctt tggattcatt cctgcatttg catagttggt
    23281 acagaatgta tgtcgcaaac tatgtggagt gatgtgtggc aatttatcct cgttatactt
    23341 attgtatttc ttaacaagac ctttcatcat gccgttgtaa tcacttgcca cttttggata
    23401 gttctttcta ttaagaaaga ggaaatcact atatccatca atctcaacac gcttatcatt
    23461 ctttcgattc gctaacactc gcttaaatgc ttgataggct tcttcaacca taggaacttg
    23521 acgttcgcca cttttggtct ttggtgtttc aatgtagtac ccaatttcag tatctctcaa
    23581 tagctgatgg tctatattga caagacgatt ctcaaaatct aaatctggaa gtgtcaaacc
    23641 accaaactct gaaatacgaa gacctgtttt taagagtatc agaatttcat cataattttt
    23701 gctgtaggtt ttatcagctt ttgcaaaggc taacagtttt tcttcctgtt cttctgttag
    23761 tacggtctta gggacagtat catcatcaag aactgctttc agttgaaagt caaatggatt
    23821 cttccgaaca caatcatctt gtatagcaat atagaatgaa gcctttaaag aacgtttgta
    23881 gttattgatg gtttgataag cataaccatt ttcactcatt ctaatagccc attctttagc
    23941 gtctgatggc ttaatactgt caatacttct tacacctaac ttgtctttct tcaaaatatc
    24001 cataagatat ttgcgtccag tttcagtgtt ttttctaacc tttggtcttt gagcgttctg
    24061 ttttgcgtaa agctggcaga gtgtcatttt ctttcctaca acatcaatac catcatgaat
    24121 gtctttctgt aactctgcga ttttctctct aagtgagata caatcacgct ttcctgctgg
    24181 tactcggtct gtagccacaa gtttccacga gtaaacaaat tgcggttctc caaatgaatc
    24241 tatatatttg tataagtatc ttccgtcttt tcgttggctc tctccagtct ttaagattcg
    24301 acctttattg tcacgtcttt tttctgacat ggcatttgct cctttccttt atggaaagag
    24361 ccttgatacg acttaatact attttatcat atacaagacc ctttggcgac gctagattgc
    24421 gtccaatgta tctataattt tttcaaattg ttttcgttta atctgaatac gattgccatt
    24481 cataatcagc caatttgcat ttttattttc ctctgccaag cgtcgtagct tgttttcgcc
    24541 aatacgaaaa tattttgacg cttcttcaat ggttagggta taacgttccc aaataggaat
    24601 gtcagtctgc ttcataaaat cctcctttcc aaatcactta tttggatttc ataaaagttg
    24661 ttttaccagc aatcgaacag ctttagcaaa gctcacggga gttccacccc tgcatggttc
    24721 tcatgtagcc atactcattg cctgcgacgg ttttatcacg ctcggactat tgactgtatg
    24781 ggagtatcat tatcacgata agaatgtcgt tgcaggcaat cctgctaaag attgcttctc
    24841 ggatcactaa catgaatcgc tcgctatctt tataagatag gtcatggcgg ttagttccgt
    24901 tggctctttt cttatcgaaa cgtattcgat tacttttatt cagttttcaa agaacaatgg
    24961 ctcgttagcc tatcaaaaca cattgaaagc tcaatatgct ttggtggaat aacaaacctc
    25021 cctgttcggg aagcgtggaa tggtttagca cgcttccacg aaaggagaga ggatattact
    25081 taatttcaaa tgacaaaatc tttgtaatca gtctggtttc cattcttcca cgtaagactt
    25141 catcaacgac catactttga ttgccatatt catctttcat aagtcgtagg gaacgcttcg
    25201 ttatgtaccc tctgtaatga tgtagaatct ggttaatcgc ttcggtatcg ccatctgttg
    25261 cctttacaat gagaggaaag ggaatcatag gatattgtgt tttcattctt caaattcctc
    25321 cataaacttt ttaattaagg ctagtccact ggttctatgc cgatagacag tagaacggtt
    25381 caatttcaac aggtctgcaa tttctgaatc gctcatgtcc ataaagtaaa acagcagtag
    25441 aatttcacgt ttcttgtctg gcaactcacg taatgcttca ctcaacaaat cattttcaac
    25501 gcctactgat aacccattga gtgtaaaaat ctgaaagtca gttgaatagt tatctgttgt
    25561 cgcaaactgg ctaacaagat aatcgccaac atccgaaaag gacacctcac gctttgcaat
    25621 ccttgaaaga taaagcatat aattctttcg ctcgtcttcc atagcacgtt tacagatata
    25681 gtcaaactga ttttctattg tggtctgaaa agaagatggt ttcatgtttc tcaccccctt
    25741 tctgtctagg aaaggaagtg agccttgctc gtttatctcc tttcactctt agtcccaatg
    25801 tgaaaggggg atttgttgca ttactgataa ataaactttg taaaaaagtt ctgaatagcc
    25861 aaaaaagcat ataaacagat ttatttctct gtttacatgc ttctgttatt ctatctatat
    25921 gatttataaa accacattgg tggacgtact tatctattgc agatagacga ctttttttga
    25981 caagaaccca atgtaaggaa atttattgta tatgatgtac ttcatggcga cgttgacctc
    26041 caacaaaccg ccatttggaa gtaatataca atattttaac agcgtaaata gcactaccat
    26101 ataacggttt tttttattgg cgtttagtag tgctttttat taaatataaa cctataaacc
    26161 atataacacg tttttctata cctgttttta attcagtagg aacaataaaa tgtatagagg
    26221 tggtctacta tgcgtaaaaa agaagataaa tatgatttta gagcctttgg tttagccatt
    26281 aaagaagctc gattgaaacg aggtttaact cgtgaacaag tgggagcatt gattgaaatt
    26341 gacccacggt acttaactaa tattgaaaat aaagggcaac accccagcat acaagttctt
    26401 tatgaccttg tatcgttact tcatgtttcc gttgatgaat ttttcttacc tgctaataac
    26461 ttggtaaaaa gcacccgacg attacagata gagaaataca tggatagctt tacagacaaa
    26521 gaactatcct taatggaatc tttagccagc ggtatcaacg aagcaagaaa catcgaagac
    26581 taattaaaag aatccataca taacggaaag agccgataaa atgagattgt attaatctca
    26641 ttttatcggc tctgcgtctt tgcgtctggc tctgtaatca cagttacttt gaactgcttt
    26701 atttcaatta aattttcttg tctgcatttc ggacaataga gggggaattt ttttaattca
    26761 gtatcttccc ttatctttaa tcgtgtttta tttccacata caggacacaa tatccacttg
    26821 tagtttataa taactatctc ctcctttaca ctttaattca aatctttatt aaaaaatatt
    26881 tcatcttatt taacaagaaa ccatatttat ataacaacat aaaatacact aagttatttt
    26941 attgaacata tatcgtactt tatctatccg actatttgga cgacggggct ggcaaacagg
    27001 ttcaccggta gtaacatggt acccttttaa ctctgttaaa caaacactac gtccatttgt
    27061 aaagaaagtt aaatcactac gatattcttg aatacaccga gcagggattt ctccactaag
    27121 aatgacctca ttatttttca attgagtgtc tacgatgttc gcacaatatt taggagcatc
    27181 gttgtatgct cgtgaaagat attcctgtgg cgcataaatt ttaaaactaa gatatggctc
    27241 taacaattct gttccagctt tttttaagac ttgttccaat acaataggag caagcatccg
    27301 aaaatctgct ggggtactaa cagggctata gtataagcca tacttaaaac agattttaca
    27361 gtccgtcaca ttccaaccat acaatccttg ttcacagcca tagcgtatcc cttccataac
    27421 tgcattttga aatgattgat ttaagtatcc aagagaaacc gagctctcat actgcattcc
    27481 acttcccaac ggaagcggtg atacagataa accaatggaa gcccagaaag gatttggcgg
    27541 cacttcgatg tgaatggtat attctgcatt ttttaacggt ctctccatat aaatgactgt
    27601 aggctctttt agttctatct ccacatgata cttttcttgc aacagtgcac taatcacttc
    27661 catttgtact ttccctaaga aagaaagtat aatttcatgt gtcgtagaat ccacgtaata
    27721 tcgtagaagc ggatcactat ctgagatttc caaaagggca tcaagcaaca tttctctctg
    27781 ttcaggttta ctcggttcaa cagttgtttg tagtagaggg tgcggatttt caatcttttt
    27841 tctctgtggc aatagttttg tatctccaag aacactattt aacttcaaaa actcattttg
    27901 caaaataaca atttccccgg aataagcctt atcgatttta cataattcac catttattga
    27961 agtatacatt tctgtaattt ttattttttc cttttccgat attctaaccg aatctcgcaa
    28021 atgcagtacg ccactataaa gacgtatata tgcaagacgt tgtctttttt ttgtatattc
    28081 aattttgaaa acatttccgc aaagttcaga ctgacctcga tgtgttgatg aataaaattt
    28141 attcgtaatc acttctataa ggttatcaat ccctatattg ttttttgcac ttccatgata
    28201 aagagggaac agagaacaat tctgaaatct tatgctttcc tcttgttcga gttccaatgc
    28261 ttctaatgat ttaccggaca tatatttctc taaaaggtca tcgtttccct ctattaccgt
    28321 atcccattgt tcagattcgg taaagttcgt cacacacata ttaggataca gttctacctt
    28381 ctgtttgatt acaatttcgg cagaaagttt ctctttaata tcctgataaa ccgttgataa
    28441 atcaattcca ttttggtcaa tcttattgat aaaaaagatt gtgggaatcc ccattttcct
    28501 aagtgcatga aataatatac gagtttgtgc ttgtacgcca tcttttgcag aaatcagtag
    28561 aattgcccca tctaaaactg ataatgaacg atatacttct gctaagaaat ccatatgtcc
    28621 tggcgtgtct atgatgttca ccttcgtatt ttcccactga aaagaggtta ttcctgtctg
    28681 aattgtaatt cctctctgac gttctaaaag cgtattatcc gtcctcgttg tacctttgtc
    28741 cacgcttcct aattctgtaa tcgctccact gttatataat aagctttctg ttaaggtagt
    28801 ttttcccgca tcaacatgag ctaaaactcc aatattaata attttcatgt gattttcctc
    28861 cattcaaaag cccaaaaggg cataaaaatc ccagtgataa atactcttat cactgggatt
    28921 tttatgcata accataggca tacaaagcat acagatattc tccggatact ttagaatcac
    28981 atgataaagg tattcttaaa ctgggtacaa aaaactaagc cctcctaaaa aaggacatcc
    29041 aattatttgt tcccactatc aaattgacag tttatttaag aataccttgc cgcatattta
    29101 ttaactcctt ttaaatagat acttaaataa tagcacgtaa gagcatattt gtaaaggaat
    29161 ctccaatttt ttatcaaaga gagtacgtga ttacaaaata gctgtaataa tgtaccaata
    29221 tttgttattc tataatcttc caattactcc cgttcttttc aagtaccaaa tcaaattgag
    29281 atacctgcgt tgctttggtc tgctggtcga tatactccac tgtcagcgat accgtgactt
    29341 gattatcctt acgattgtga ataggattta ccagttcttg aaagatgtac tcttttccga
    29401 ttggttttaa tatcccgtca ttcacatagt aggaaagttc actggctgtc gctgtaggat
    29461 agagcttgaa gaacgtcgtt aaaaactcat tgatttcatt ggttgtaatg gaatcaaccg
    29521 tcccctcact ttcaatggct tttggtttat aacttgattt cttaggtatg ttggtaatgg
    29581 tcggattctt aaccagtacc atatttccag aaccatctac atagacactc actatataag
    29641 cagagtggac ggtctttgta ttttctccct ctgtaatgag ctggtctaca ctgtaggtta
    29701 cattaaactc attgtcgcca gttggctcta ccgtccatat ctgaaatcct cttacagaag
    29761 acgatacagg aatatctttg cgtactgtat caacattgag agcttgaagt tcatctgtca
    29821 gatagccttt tagactttcc attcgattat caatggactt atcggattgc tcccatgaat
    29881 agtagacttt cgcaaagttc tctacaaaat tttctacatg atgagtatca acgtattcct
    29941 tttctatgat agttgtttcg tgaatagtat gagtatctat agctgtaaag tgcttgaata
    30001 tcgcaaagct gaaactaagc cctaaaagta cccacaaggc aatcacaacc tttttatgag
    30061 gattgacctt atagacacga ggtttctttt cctttggtat ctgtttttct ttattctgat
    30121 tttttctaaa tttcatcatt aaatcttcct ttctcattgt ttgattcgtc ctgctcccac
    30181 taaatgctgt tgccagtagg ggcttgttaa gtcggcataa ccgattgggt cgcctgcatg
    30241 aaacatacgg ttattgccaa ggtatatccc aacatgagta atataagagc cagcgttata
    30301 ggtagaatga aagaaaacca aatcgccagc ttgtgcttcc gatagtggga tatgctgggt
    30361 cacatcatat tgctgttgtg cggttcgtgg taagttaatt ccagcttttc catacgtcca
    30421 ttgtgtcagt ccgctacaat caaaagaagt agtcggggaa gctccaccgt aaacgtatcg
    30481 ccagccctca tatttcagtg cttcgtccat gatggcttgt accgtatcat catcaaactc
    30541 tgttgtgaca agatactgcg ttaccagttg cacataaaac atattgccat agttgtatcg
    30601 ccagccccca ttgataggta tggctatggg attggggtaa gacacttttt cgccacctga
    30661 atactctttt gagaaacttt gagccagttc aaaggtatat ttatttccac gattagccac
    30721 ataccctaag aaaccaccac cataattgta ggactggata accgattcta aatctacact
    30781 gagcctttcg ctactggcta ataattcact gaaatacttc acaccttgct taatggattc
    30841 ttctgtactc aatgaattag gtggaagacc gagggattcc gaggactgca taacatcttc
    30901 cgcagtaccg cccgattcca cctgtataat cgcaagaagt atgttgacat attcttcaac
    30961 gccatattct ttggcatatt tttctaccat aggcttatga gccagcactt ctgcggaaac
    31021 attcacacct ccataatgaa tattggaaat tccgctgtcc tgttcatctg aaaataaaat
    31081 ggcaacaaac agaagcagtg agaagaccat caagaataat ccagaaccac caatcactaa
    31141 agttttcaac ttcatggttt cttaccgact ttcttaatgg tggcggtttt gattggtggt
    31201 ctacttcttg tattttgtag tggtactctt tgaacagtag acggacgttc ttttgtgatt
    31261 ggacgttgtg aagttctatc tgctgtagtg gttgaagttg ctggcttttg aacggttttt
    31321 tcttgaacgg tattgccttg gcgttccact tttggacttg aaaaatcgga cttaactgct
    31381 ggacgctctt gtttggcttg ttgagattcc ttatatgaag tctgaatatt agactgtttt
    31441 gaggtctgtt catcatgata ttgttcttgt cttgtagtcg gtctttcatg aacagaagaa
    31501 gcaggctgtt ttttctgttt gacctgttcc atttcagagc gacgcttcgc aatggttttt
    31561 cgcctttgtt cctgctgttc cttgcgtcca ctggctctgt ccgctttggt ttgagaaata
    31621 ctactggtta aatcacggac attctctttt actttggatt ttccttgata tactgcatat
    31681 cttgcattgg tcggcaaatc tttaacctgt tctttcaaac cactagcagt gtctaccatt
    31741 ctgtctttgg tatcagctac tgtaccgatg gtttgaccga tacgttttcc aagtgttgat
    31801 ttttcctttc cgtctggtcg ggagtgatct gcttgtgtcc ttgcagaact ccccgaaccc
    31861 gactgtcctt ttttacctgt aacaatggca gacccagccc ctagagtagt catggaacgt
    31921 ccaagtttcc gctgtagacg gtgcatgtga gcgtgcataa gcatacgagg ttttctcatc
    31981 acacgacttc ccacactttg agaatcgtta ctctgtagag aaaacatact cattaaatcg
    32041 cccagcttga agtagattcc tgcaaaggtc acaatctgta gaaaagcaat caaaaagaac
    32101 ggataaccag ccgataaggt atagagcatg gttgaaatac taaatgctgt cgtaataatc
    32161 aatgtgattc cagctcgtgt caaaatggta ttaaagagct ttgttatggc tcgttttgac
    32221 ataccatcaa atgatggaat catgcttaaa ataaagctca caggcagaaa catagcatag
    32281 atgataaaaa gtacctgcga gaaaatcatg attcctgtta ataggaatac aaatatggaa
    32341 atcccaatat tgaagacaaa taggaagaag actgtaccta aacggttaat ggtctttgta
    32401 atggttagat tggtattgct tctgtcttca atttcttccg caacaatttt ttctctgtct
    32461 tcgccattgt tggaatctgg gctggtggag agcaggcttt ccacacggtc aataccgata
    32521 ctttcaatgt ctgaactgtt gtattgaagc agtagccacg gttgctgaac ctgtatggaa
    32581 aacaggctat ctctgattaa gtccacgctg tccttgcctt gactatcgga atggggcatg
    32641 acaatcttcg tgccaagtga taaactggca ttactgatgt ctgatgaaaa gtcattgatt
    32701 tttttaatgt agtcgggagc gtaggcaata aaggaagccg ataggataaa caccagcaca
    32761 aaattcataa tggcatgaat tgcctttgtg gtttctctct ttatcagtcc cgtataggca
    32821 acataaaccc caagaaccaa aatcaagagt aagaggaatc caacatagaa accctctgtt
    32881 gaaaatccgt ttgcactcac accagctaag gtctgcatat tcttaccaat ggaatctgct
    32941 gtagcggaaa tgaagtctaa ggaataggct tcctgtacta agtaacctgt cgcattggaa
    33001 acatacaaac tgattgtcca aataaaattg gtaatggcat atagtccata catgacctgt
    33061 tttccaatcc cgtccgacca gttccacgga agccagcccc agctattatc cacataaaaa
    33121 tccagttgat agttttcaag tgggtatcgg ctgtattcat ttgccacatt gaccgtatca
    33181 tctaccaagc ccgcagcttg aaccaccgtt cccagcatgg ctaaaagaaa aatggcaatc
    33241 acaagtgtga aagccactgt cattgccact ttacctagac gtttcagcgt ccagtttgat
    33301 tttattctgt ttactattga tggtttcaca tttacacctc ttttcgcaca ggtggtctgg
    33361 tatcaaaggc atggagcagt tcttcaaata cagggtggaa ctgtatcaca ccgacacgac
    33421 catataaatc actgataagg cattgcccgt tttccaaatc acgcaatcgc ttctgattgt
    33481 tttcgtcctc tgggtctaca ccaaaaaagg ctaaggtctt tttaatctcg ttaaggtcag
    33541 tggaacgaaa tgcaaatttt aagccgaggt tatttttcag tttttcatct aagaggtcgt
    33601 ctgtattttg ggtcacgaaa tataccccag cgttcatagc acgaccagcc cgaaccagct
    33661 tcatagatag tgtttttcct tgtgctacct gtaaaaagct ccatgcttcg tctaaatcta
    33721 caatcttgaa aatgcttcgg tctgtatgga taaagtctaa agcaaaggta ctaatgacaa
    33781 tcagcatagc aacggataaa agctccatag tggtatattc ctcaaaggaa gtttccttgt
    33841 cgggaagtac caagtccgca acctgtataa tgttcagttg tttttctaag ctgatagact
    33901 gctccacata accattactg aataataaat gtgcaaagtc atagtctgta aaactttcga
    33961 tatggtcggc tatactggta cttagtggcg tattctcaac ccgtaattcc tcaatcactt
    34021 tcatcaaccc tcgtacttca ctattggtta ctgcacgaat ggcttttcta aggattggga
    34081 agcgttcccc atcacgagag gaaatccccg taaggaatgt cagaatatca atagccagtg
    34141 attcagaatc tttgggattt ttcataatca cataagggtc aagtaagcct ttgtttttct
    34201 catcagaagt cagagtgacg atattgattt catgggaaat ctctggcaag gtttctttcc
    34261 atctgccacg ttctgctttt gggtctacaa tcactgcttg tgccccataa agcaccgcat
    34321 aatagacgat aaggttattc gcaaaggatt taccaccacc cagcgaacca acaaaagccg
    34381 acgctaacgc attggttact gaacccttaa ccccttgact ggcaagagca ggtttcagat
    34441 agacattgcg tccagtatct aagctgtagc caacataaat cccctcattt tcccccagca
    34501 tttgagtagc accaaaacct aaaccagcga ggaaatcaga ggtcacgtat tgaatataat
    34561 cattcatata acgcttgctg gcaggtaaaa attcttcatg taagccgagc atatccccaa
    34621 atggtcgtac cagttttacg cttaaatcgt cataaaaatc tttcacttca ttacaacgac
    34681 gtttgagttc gtcaagatca tttgctgata cccttaccac ataagacagc ttgtacatag
    34741 attccttgct ttggtctaaa ttggtttcca gctcattcac actttccaga gcttccgcca
    34801 cattggagct ggtttcatta tcactttgcc aagcgtggtt atccaagtct ttcagttctt
    34861 tctttttatt gcggacagta gatagggctt tacgattcgc tacaatttcc acattcattg
    34921 acgtatcaat cgggaatgta aattgctgtt gctggtagta gaagatttca gaggacggga
    34981 agtccagttc tccgacaatg ctgttaatgg taaagtaagc tacatagacg gtttcatctt
    35041 cctgctggat tttcaaatat cgctgttttt cttccaccaa acagcgagta ggcttaatca
    35101 agtcatagta tttaatcagc gtttcattat ccagcttttt ctttgataga tggtactcat
    35161 actcttcata ggcagtgcct gtctgtccgt aaaggtgttc aatcagatag ccgaagtcgt
    35221 ccttatctaa cctgcggatt ttgaaacgac gagagatttt attttctaag agcttttcca
    35281 tcttctgaaa acgcaggatt tcatcattac tcatactaac aaaatcgccc atcagcttat
    35341 ggttcacatc atagacaaaa tcagacaaag cattttttgc ttcaacggta agacttttca
    35401 tagaaaactc ctgatcgttg agaagcaact taaagccgat aaagaaacgg tagttcactt
    35461 gattttcgcc aatcatggat attaaagcgt ctgtctgttg gtcgattttg tcataggcaa
    35521 ccgctttgag cttgccagtg acttcatttt tggaacgctc ttgtgcagaa cgtatgctgg
    35581 attctgtact gatttgtaaa gcatgaattt tgccatcacg attttgtgcg ataagctgtc
    35641 tgaaagaatc atgcacttgt attttctgtt ctggacttag aaatgagtaa ttgtaaggaa
    35701 caagctcata gtaagcataa cattccccgt ctttattcca gacgagattg ttttcaatgt
    35761 atttaattgg atatgccata aaattcactc ctaactgctg taatggcttc ttgtggctgg
    35821 tttctgccaa gcgttacttt ttttcctgca taggtcagct ttggtcgcag tgcataagca
    35881 atgacagact tcaaaaatcc ataaggcttt ttaccatcaa aagtttttgt agacataaac
    35941 catgtgaaag ccacaggaat cccaaagtat ttgagaaatg ctccctctat catggaaaga
    36001 gggggcaagt tgccaagtat catcactgca aagagtgaca cgacaaacca tgtcatttgc
    36061 gtaaaggtta tgggaaacgg aagtctaaaa tcattgatag aatacagtac cttttccaca
    36121 gaccagatac tggtatagct tcgtattttc ttcatgtaat caatcctttc ataaaaaata
    36181 ggggtagctg attgagccac cccgtaaaat agaaaatctg ccagtagtaa tgtaccgaca
    36241 gatttaatag acgatttcaa aaatcccatg attggttgag ataaacgttc ctgaaaggtc
    36301 taaatcccga ccataggctt gataatcaat atagttttga agactagctg gtacttcgcc
    36361 taaagcaccc gtttcttcaa tgtagtagcg tgccacgtca tacatatcat cacaatcgga
    36421 atgaatgata atatcctctt gatgttcgct tagttcttca atgcttgaaa aatgagtgag
    36481 cagagcagat agctccgatt gtaattcttc gggtaattcc gataccattt cccatagtcg
    36541 attgagttcg ccaatggaag tgtattcgtc aaccgtaaag ggtaactcgt agtcatgaat
    36601 ggcgtattcc tcatattcat cattcaagcc gattttctct ttgacttcct caaagtcaat
    36661 gggaaaggta aaccacgcac cgaccaattc gccctcattg tatttgccta aattcgcaat
    36721 atagacttgc atatcgtcca tatattcacg tcctttcttt gtagagattc aaaaatccct
    36781 accgcacttc gtttggtgta ccattccttt gcggaacata agaaaaccac ttatattcca
    36841 caaaagaacg gttttattta agcaccaata atgcgattga atagctctag taaaatgtct
    36901 tttactccag cagcgttgaa gactaagcca accgcaataa tcgcaataat taaaaagcca
    36961 atcagtttgc taaactcacg cttgaagcca agatacaagc caatcacaac gattgctaaa
    37021 agcaccagtg attgagcgtt tgatagaaac cagttataaa ggttttgtcc aaaattcata
    37081 aaaatgttct cctctctata ttcaatgaat ttgtatttga gttatttttt tgttgttatc
    37141 acgtcctgtt cttttactga ctgttgcttc aaaatctgct tgtgtcggtc tgtcagtttc
    37201 gcatggtcga gaatgtcttt tacaacctgc gtctggttga tttcatcaag tttaatcgca
    37261 acctttaagg tcggggcaac ttgatgagat agccagttca gcgtcctttg gaaggagtaa
    37321 ggctctggtt ttgtggttag ttttaatcgt tcacgattgt tcccaataaa ccaagcccat
    37381 tcttcattca gtttccaatc agaacgaggt ttggaatcgt ctttatctac aaaacggata
    37441 taccgattga taattttaaa ggcggtatgc tctggattgt catagacgag taaatcacgg
    37501 actgcataat aggcacgctc atttttcaat cgaatctcaa aacggttttt tacttctgcg
    37561 tcttcaatgg gaatatcatt tttcttgtac tgctcgtagt ccttttcata gatacagaaa
    37621 taaacttcac tttgtaatga accgatatag agggtgtttc ccatacattc cttttcctct
    37681 ttgcgtacca gttcgccact gcgatagctt ttaaaactgc ggaagacgga gatacattct
    37741 tcctgttggc acttttcagt gagtacaggg atatttaaaa tccctgtctt atcgttaatg
    37801 gcaaggtcaa ggcgtttcat cacaccgcca gccaccaaaa cgtccataaa gaactcatac
    37861 cagcttcttt gttgtgccag aagatagctt tcaaattgtc tgcacccacg acctttcaat
    37921 tccaccagaa ctcctttgtc cagttcatgg gagcaaagga cgaatatgtc gcctaaagca
    37981 taatgctctg aataagaata gaaaccatag tcctcatgaa gaaaatagga cagtttcagt
    38041 tgtaagatgt tttcgaccac ctgctgtacg tctgttgtcg gaaagcgaat tcttacataa
    38101 tcaaacagca tttcaagggg agcgtcggga ttgaagcgtt ccagagcttc ccaaagggac
    38161 tgctgtaaat cctctgatgg cttgactttt cctgtttcaa tatcgcttag atactgcctt
    38221 gtaataccag tcgcaacagc taaacggttt tgagatagtc cataagccaa gcgtttttct
    38281 tttaaatgct gtaaccaagt ttgttcattc agtaaaaatc cctccaatca aaaaggcgta
    38341 tgtcaacttt taaagcccat ttgacatacg ctgaaatttt gtaaatccct tgtaaccaaa
    38401 ggattttcta atgttttttt gactgtttcc tgtcgatttg tacccccctg ttagatacgg
    38461 ggggttaagt gctggcgtgg ctattgccac accagccagc aagatcagtc cacacctgcg
    38521 acttccgctt cgcacgtcgc ctgcgtggac tgtctgctgt tggataactt tttaatttcc
    38581 tccaagaaat catatccttt tggtacaagg ggagtataaa actctgatat gacacttgtt
    38641 cctacatcaa catagccacg acctttgatt cgctttaaga agaaatcctt ttgtacgtca
    38701 ctgccaaaca tcatgccata gcccatttca gacatacgac ctaaagccac tctgaaatta
    38761 aactgatcac ggattccgtc gcctaaatat tttgcgtctg gacgttgaca agccagtatt
    38821 agaaagaagc cagcttgacg acctaacatg acaatctgtt tcagcttatt cataactgcg
    38881 gtgttttctt ttgttcccag catttccatg aaagcgacgt attcatcaaa gattaagaag
    38941 tgtgccggga gacctaagta agcataattt ttgccagtct tatagttctt catctgcttc
    39001 atttcctcac tacgtttcat catttcttca tagaatgttt caatgcaaga aagcaagtct
    39061 tcttttctat agtagacatt tgccatcaca gaacctaagt ccgcaagatc agcatttttc
    39121 gggtcaagaa tatacagttt tgaatctgta tgaagcaagg cttcaatcag tgtcagtata
    39181 aagtaagttt taccgccacc tgtaccacca gcaatcaaca tatgagggag cttatcatat
    39241 tcccaccata cgtttttcat taagcgaagt ttaccatctt tagcttctac ttcatcaata
    39301 gaaatacgac tggctatggt gtcatagagc aaagtatatt ccacatagga atcctttaac
    39361 tctttatccg tcagctcaca gtacaagcca ctctctaatt tcttttccaa gtgtaagagt
    39421 tggtcttgat attttcccag cgtgatttcc acccgtatct gtatcaagcc atttttaagt
    39481 cgataataca ttttagggaa gtaggttatc ttttcctttg tacgaccagc actatcttta
    39541 aagaaaccct ctgttttgac ctgttcagat tcataccact tgttttcaag tatcatcttt
    39601 gccagttttt gacggtggta aagttgttta accgtatcat agcgaacccg tttgaataca
    39661 aacgctacca gcaagcagat aagaattgcg acactgaaac tgataattaa atagggaatg
    39721 tcaatcttat ctgcttgtga taggttaaaa tcctgccagt tgatctgctg gattgtcttc
    39781 acatgaaaca gtccgacaac cagcaggaaa acaggcagga gtgacgctat cgtaaaatga
    39841 aagactaaat ctttaccaga tgggcgaatc cttttaccac gctgtttcat gcgaaaaagt
    39901 ctcctttcta cctagcgact atttgtcttg tgtcggttct ttctttgctt gtggttgagc
    39961 tttgaatgaa ctagaatcct ttgtcagcac aatatcgtct gccttgatat accagtcaac
    40021 atctgctcct tgataggtgg cagtagcaac ggtgtccgca atgggattga taagttccac
    40081 ccgtgcgtta taatcaaact ctttcaaagg cacgctggca ggaatactta cttgaatcat
    40141 gcgtccttgt cctttggatt ttaagtcata ggtacgttcc ttgatttcat ctgaaaccga
    40201 cccgtcttca ttttggattc tcacttcacg acgtagagca gagaatttca attctccaaa
    40261 agtcgtgtct ttatctaata caatgccatt tgctaatctc atcatttttc ctctctttct
    40321 ttattctttt atcatgtcgt cagcatgtaa aaggtaattt gtaaaaccac gagtgccgat
    40381 tttgtagccc tctgcggtaa tacgtggatt gactaacttc acacgttcct caaagccgaa
    40441 atgtttttcg ccagcttcag caggaagcac caccacaata tcatctgctc tttgaacatc
    40501 agaatagaga ttatagcttc ttgataagac agttagccgt ccgttgattc ttcgctgaac
    40561 gactttatcc tcgccagcaa attctaaatt gccgaatgtt ttttccatgt tgggaatcac
    40621 aaatttaagt tccatatttt tacctatcct ttctttttta ttggctgaat gaatgtttga
    40681 tggtcttaaa gagtggggaa cgaccttttg attcttgatt ttttgttttc ataagttcac
    40741 ttcctttcaa aatcgggtaa aaaaatagac acctcatttt ttgaagtgtc tacctattaa
    40801 atattcaaat tttattggaa gtatctttat atcttcactt ttcaaggata aatcgtcgta
    40861 tcaaagctca ttcataagta gtaaattagt agtaaattga gtggttttga ccttgataaa
    40921 gtgtgataag tccagttttt atgcggataa ctagattttt atgctatttt tgtttttaaa
    40981 aaaagtatta tttgtaaaat ataaaaaaaa atttcttaat atttgaataa aagaatagct
    41041 taatttaaat gttattactt tataaaaaaa taataagtac aaaaaaaaca atatatggca
    41101 tataagttgc tatataaata ataaggaggg aacaattata aatgaggcaa agaataaaaa
    41161 acttaattga aaatgaagat aagaaaaatc ctttaacaga tgaagcgatt tcaagtaggt
    41221 taaatattcg aagagaagat gttacctttt tgagaaatga gttaaaaata gaagattcaa
    41281 gacaaagaag aaaagttgtt ttaattaaag ctataaaaga tattttaaaa gaagaaaaaa
    41341 ctataaataa aaatgatata acaagaagat taaacagcat tgggtttaca gtatcaagat
    41401 ttacagtcat acaatactta aaagagattc aagattctgg tgacctaaaa cttgataaag
    41461 agagtgatga taaaaaagaa gaaataatca ataatagaga aaataaagat atagcatttg
    41521 ataaactcat aggtagtagt ggtagcttaa gtacaattat aaaactagcc aaagcagcaa
    41581 ttctttatcc acctcatggt cttcatacta ttattatggg gccaactggt gttggaaaga
    41641 gtgagttagc agagtgtatg tataaatttg cagtagaatc atccagattc cctaagaatg
    41701 caccatttat agtttttaat gcagcagatt attcagagaa tccacagctt ttgctatcac
    41761 agttatttgg acacgttaaa ggtgctttta caggagctga tgagaacaaa gaaggattag
    41821 tatcaaaggc agatggagga atattgttta tagatgaaat acatagatta cctcatgaag
    41881 gtcaagaaat actatttcaa ctgattgata aaggtatggt cagaaagtta ggagaaacaa
    41941 gactaacaca taaaatagat gtaatgatta tttcagcaac ttctgagcca atagattctc
    42001 atttacttaa tacatttaaa agacgtatac ctgtaacaat tgaaatacca gaattagttg
    42061 caagaccatt aaatgagagg tttgacatta taaataattt cttcttaata gaagctcaaa
    42121 gaatgggagc aaacatacat ataaagtcag atgtattaaa agctcttatg ctttacgatt
    42181 gtataggtaa tgtaggtcaa cttagaagtg atattcaagt agcttgtgct aggggactat
    42241 taaaccaact tacaaacaaa ctaaaagaag taaatataag catatcagat gtacctggat
    42301 atgtaaaaca aggtcttata aaaatcagaa attgtagagg taaaatagaa aattatgtag
    42361 atggggattt aattatagat tcaaatcttc aaggtgagat agaaaagaaa aatgaagata
    42421 tatatacttt tccagatgaa atatacaaac ttactgaaag aagacatatt gatttattgg
    42481 aacaaggttt agattttgat gttataaata gaatcattgg aggagaatta gaaagttgca
    42541 ttcaaaaata cataaaacaa gttaaaaaag tttctattag taacaatctt ccagatataa
    42601 gtccaattgt tggaaaatat atcgtagagc taacttatga agtaatcaaa atagcatcac
    42661 aatacctaag ttcactagaa ccgaatctac atttatgttt agcagttcac ctaaaagcag
    42721 cttttgagag attaaaagaa ggaaaagtaa tatcaaatgc tcatttgaaa gaaataatag
    42781 gaaattatcc actagaatat tctgtagcag aaaaggtagc tcaatattta aaggaaagat
    42841 ataatataaa cttaccaaaa gaagaaatag gtataatagc aatatatttt aaaatgagta
    42901 gtagaagaga tatgtctgaa caaaaaaaga taggtgtact tcttatggct cacggaagag
    42961 tagcatcttc tgtatgtgag gttacaaata aactattagg aattaatcac gcaaggtatt
    43021 tagatatgcc atttgagaag aagccggaag aaatgattga agaagctact acaataataa
    43081 aagatattga tgaaggtaga ggtgtgatta tattagtaga tatgggttca ctttcatatt
    43141 ttggagatat tatttctgga aagactggaa ttaatataag gacaatatct agatttgata
    43201 cattgctttc tatagaggtt atacgaagag cagttttacc agatttaaca atagacgaga
    43261 tagttgatga agttaaaggt atagagccac aagtaaaaga aaaggttagt aaatgtaaca
    43321 atacaaaagt agattttaga aaacctataa ttttaacatt atgtattact ggattagggg
    43381 gagcttgtaa gataaaaaaa atcctagagg aaaatattaa aagtataact gaaaaggttg
    43441 atatagaacc tataggtatg attgaatatc caagtcttga tttagagata aaacaaataa
    43501 gcaatgataa aaatgtaatc gccatagttg gaacaattga ccctcagata gcaaatatcc
    43561 ctttttttag tctagtagac ataaatgata aagcaaagtt aaatcaaata aataacctaa
    43621 ttgatacaaa tcaaattggt gaaacaaata aaaaatatga attatcagat ttaatacata
    43681 aagaagatat atttgtggat ttacaggtta aatcaaaaga agaagttcta aaatatatga
    43741 gtaaaaaact tagagagaaa ggttatgtga caaaaaattt ctataaaaaa gtagtggaaa
    43801 gagataactt atttccaact gaattatcag atgaaatagc aatacctcac acagatggaa
    43861 tagatgtaat taggccagct ataagtatag ccatcttaaa gaagcctatt gtgtggagta
    43921 aaagtaaagt aaaaataata cttttattag ctgtagacca aaagtgtttt aatccattaa
    43981 gcactctact ctcatttata gaaacagatg aatttaagaa gataaaaaac attaaagata
    44041 gagattttat tagggagatg atattagatg gagtcaatga aaataattag tagcaattta
    44101 atttttaaaa atattgaagt gtctaacaat gaagatgcat taaagttttt gggacaacgt
    44161 ttgtttgacg aacagtatgt aaaagaaagc tacatacaag ctgttgtagc aagagaaaaa
    44221 aagtatgcta caggattacc aacagaaata tacggagtgg caataccaca tacagatata
    44281 gtacatgtaa atgagccagg aatagcaatt ggaatactga ataaaccagt aaaatttata
    44341 atgatgggta cagacgatac tgaaattgat gtaaaagttg tatttatgtt agcagtgaaa
    44401 gagccacaag aacaattgca actattagaa aaacttatga ctatatttca agataagaat
    44461 atactaaata atattgttga cttaagtgaa gaaagtgtga gtgatttatt aaactctaaa
    44521 ttgaaaaatt aaacatgtaa agtatattaa aaatgtaaaa ttaaacatgc aaagtatgtt
    44581 aaaaaatata aaaagattat taaagatttc taactataga tatctttaaa ataaaataat
    44641 ttcaatgggg gtgactaatg tgactaaaaa gatattagtt gcatgtggaa caggagtatg
    44701 tacttcaaca attgctataa acaaattaaa gaaagcactt caagacattg gtaaattgga
    44761 tatggttaat attacacaat gtaaagtagt agaagttgca tcaaaagcac ctgattatga
    44821 tttaataatt tgtactacac aagtatctag tagtataaaa acaccagtga taaatggatt
    44881 acctttttta actggtgtcg gaatggataa attaatcaat gatgtattag aagagttaga
    44941 attataatta aaaaaattaa ggaacatggt ctctaaaatc ataagtttta gagacttaaa
    45001 attaaataat ggagggtctt tatatggaga tattaaattt cattgttggt ctaggtgcac
    45061 aagtaatgat gccaataata atatgtattt ttggtttgat attaggtaca aaactaggaa
    45121 aatctttaag agctggttta actgtgggag ttgggtttat aggtcttaac actattatag
    45181 ctttgctaac agataattta ggtccagcta cacaacaaat ggttaagaat ttagggctaa
    45241 gtttatcaat aatagatgta ggatggccag cagcatcagc tatagctttt gcatcaacag
    45301 taggggcttt aattattcca ataggtcttg tggtaaatat agttatgcta attactaata
    45361 ctactcaaac tgttgatgta gacatatggg attactggca ttttgctttt acaggagctt
    45421 tagtggcagg ggctactcaa agtgtaatgt ggggagtatt tgcagcagta gcaaatatgg
    45481 taattgtatt ggtaatggca gatttaactg ctccaggtat agaggaatat ttaggtatgc
    45541 caggtatatc tcttcctcat ggatttacac aggcatttgt tccaattgct atagtagtaa
    45601 ataaattatt agacctaata ccaggtataa ataagattga gataaatgct gatacattgc
    45661 agaaaaaatt tggattattt ggagaacctt taataatggg gtctgtaata ggtgtaataa
    45721 ttggtattgc tgcgaaatat gatataaaag gaatattaca attaggtgta actatgggag
    45781 ctgtattaat attaattcca aagatggcag cattattaat ggaaggatta ttaccagtat
    45841 cagaagctgc acaggaattt attgagaaga gatttaaaaa cagaggcaag atttatatag
    45901 gcttagattc agcagtagga ataggtcatc ctgttacatt atcagttgca ctagtgttgg
    45961 ttcctttgac tatacttata gcagctatat tgccaggaaa taaagtttta ccatttgcag
    46021 atttagcagt aattccattt gcacttgtat taatcgtacc aattacaaag ggaaatgtgt
    46081 ttagaacatt aataatagga ataattataa ttacttctgg attattaatc gcaactaact
    46141 tagctccatt atttacccaa atggctttaa atgctagctt taaaatgcct gaaggagcaa
    46201 ctatgatttc aagtatttgt gatggtgcga atccattaag ttgggtattt gtaaaagtta
    46261 tgaactataa agttattgga ggagtagttt ttggagttat agctttagga atggctattt
    46321 acaataggaa tagaataata aatgaaaata aaaagctatc tatagaagaa taaggttaaa
    46381 acaattttat taaggagtgt ataggttatg aaaaaatata aatatgattc taaaaggtac
    46441 ttactcagtg ttacaatacc aggatttttt atgattttaa tattgattta tgcactgttt
    46501 aataactata taaattttaa tttgaatata tatagtttgt taataattgt ctgtacttat
    46561 ggtatattca atacttttat atcatcaagt aatcctaaaa aaattattat tgacagcaaa
    46621 tgtatacatt ttacctcatt tatgactaca cataagtatg aaattaaaga tatagaaaag
    46681 ataagaataa gagaatttta taataaaaaa atttatctaa aaattaatga tggagacctt
    46741 tttaaaggta ggtattggat aagaacaaat atgtttaata atagtatagc attatatagt
    46801 tatttcattg agttggaaga gtgcttatat ccagattcac taagatttag aaataaaaaa
    46861 tttgagaata agaatatata aaaataaaaa attgaattga agcctaactt aatctttttt
    46921 gataagatat aagtttgaac tttatactaa atttttagaa ttttcaatca agacaataac
    46981 ataatagcta agcaattaga acacaactaa gatgtataag gcatgtatat gatgaatggg
    47041 aggatttaat taatgttatt agagaaatta agaaaagaag tattacaagc atctttagac
    47101 cttttaaact ataatttagt gacattaaca ggagggaatg taagtgggag agatgaacaa
    47161 actggctata tagcaatcac cccaagtgga atggattata gaaatcttac accaagtgat
    47221 attgttatag tagatgtaga tggaaatatt atagatggaa aatggaaagc atcagtggat
    47281 ttatcagacc acttgtatat atataagcat agagaagaca taaatagtat aattcataca
    47341 cattctacat actcaagttg ttttgctatt ttaaatgaac ctatagaatg cgcaagtaca
    47401 actcttgcaa atgaagtagg gggttcagta ccagttgcaa aattttcacc accaacattt
    47461 aaaaagatgg gaaaatgtgt tattgaagca attggtgaca aaagagcatg tcttttagca
    47521 aatcatggcg taattgcagt tggtccatct gtaggacatg ctcttacagc cgctgtaatg
    47581 ttagaagatt cagcaaaagt atattactta gcaaaatcaa ttggcacacc agtgctactg
    47641 cctgatgaag aaattcaaag agcgagagat gtatttttta atgtctatgg tcaggataaa
    47701 taaattagat aaaattttaa aagtaatatg aaaatataga tattcaatga tattaaggag
    47761 atttataatg atataataag tttaatagtt aagaagtaaa atatgaaatg taatagtata
    47821 taaattcatt taagactaaa aataataaga taaaataaaa aggagtctgt atttctatgt
    47881 attacttatt aaacaaaagc acaaatcctt actttaattt ggcattagaa gaatatctat
    47941 ttttaaatga taaatataat gatgatatta taatcatatg gagaaatgaa gactccattt
    48001 ttataggtaa aaatcaaaat ccatatcagg aagtacatca tgatgttatt gaaaagggag
    48061 aaattcctat tttaagaaga atatctggag ggggcactgt ttatcatgat ttaggtaata
    48121 tcaatatgtc attcatacaa aaagataggc aacttcatga gatagatttt ttggagcata
    48181 ctaagtttat gcaaaagatg ctttcaacct taggactaga tgtatctata actgaaagaa
    48241 aagatttatt tttaaatggg aaaaaaattt caggctctgc acagtctata aaaagaaaaa
    48301 attctcttta tcatggaact cttctttacg attcagattt aaataaatta actaaatatt
    48361 taaattcaaa taaagcaact gaatcaaatg caactaaatc tgtttcaagt aaagttacaa
    48421 atattaagtt tttactcgaa aaagatataa attacttttt agattattgt gttgaatatt
    48481 taaaatctaa tataaagaat ataaaagaac ttgaattgga tgaagaagat attaaaagtg
    48541 tatacaatat aggagaagat aaatatagaa agatagagtg gacatatggt aaaacgccga
    48601 aatttcaagt agtgctacca tttaataatg aaagtaaaat aaatatacat gttaacagat
    48661 ggaaagtatc aaaatttttt atttcttatg atgataatgt aattaatatg gataagttgt
    48721 tagatttgaa tttctttaaa gaagaaataa aagagagtat tttagaaaat tatccacatt
    48781 acatagattt aatcaattta atattttaat ataaaacctc taatttgata aaattgtctt
    48841 atatcaaact agaggttttt aatattcttg catattaaca accattacaa aattgtaaaa
    48901 ttaaaaacaa cactattgaa aaatcaatat atattgtaat aaatatatgt gtttttcatt
    48961 agcaaataga cattaactat ttatacaact gaaaatttaa tatataatta atcagatggt
    49021 taaaataaaa atattaaaag ttttaataaa tgatggttaa aaataaaata cgaggtgata
    49081 ccatgtacag tgttataatt gttgcagcgg gaagcggaag gagaatgaac ttagatataa
    49141 acaaacagtt cataaaatta agagaaaaag aaattattgc tcatacaatt caagtttttt
    49201 atgaaaatat aaatatagat gaaatagtgg tatgtataaa aaaagaagaa gaggattttt
    49261 ttaaagaaaa tataataaat aagtataact ttaaaaatat aaaaatagca tatggcggaa
    49321 aagaaagaca ggactcaata tataatggtt taaaaaaatt agatgaaaat tgtgatatag
    49381 tacttatcca tgatggagca agaccatttg tcgaccatag aataatcaac gaatctataa
    49441 aggtagctaa agaaaaaaag gctgtggttg taggtgttcc agtaagtgat actataaaga
    49501 tagtgtcaga tggaacagta caagaaactc cagaaagaaa tttgttgtgg gcagcacaaa
    49561 cccctcaaac ctttgaatat aatctcataa tagatgctta tgagcaagca tataagaaca
    49621 attattatgg tactgatgat tcaatgttgg tagaaaatat aggtcaaagt gtcactatgg
    49681 taatgggttc ttatgaaaac ataaaaataa ctagtccaga agatttaaat atagcagaac
    49741 aaatattaaa tatggaaaaa agagatgaag ttaacagtag aaggagaatt atatgagaat
    49801 aggattagga tatgatgtac acaaattaac agagaataga aaacttataa tcggtggtgt
    49861 agagatacca cacgataaag gtttgttggg tcattctgat gctgatgtat tgatacatgc
    49921 aataatggat tctattttag gtgctctagc attaggagat attgggaagc atttcccaga
    49981 tacagatgaa gaatataagg gagcagatag tatgaaatta ttagaacatg tgtataatct
    50041 aataacaagt aaaggatata aaattggtaa tatagatagt actataattg ctcaaagccc
    50101 taagatggct ccatatattg agagtatgag aagtaatata tcaaaagttt taaacacaga
    50161 cattgataat ataaacataa aggcaacaac agaagaaggt cttggtttta caggagcaaa
    50221 acagggaata gcttcacaaa gtatttgttt attattgttg acaagtcaga ataattaata
    50281 tattattagt aataagttta atggatatca agtaataggc ctatatagaa tatctataat
    50341 gtatgaattt aagtgaaatt aatataaata taaaattagc tatgataaag aaatagtaaa
    50401 atgatgaaat cttcacagag agaaaacggt ggtgagagtt ttcaagagga agtctttgaa
    50461 cctgtctttt agcttaaagg tataaaaatc tttacggtag gaaccgttat attcccaaaa
    50521 gagagctatt catatagcta actagagtgg taccgcggaa attaaacctt tcgtctctat
    50581 acttgtatag agatgagagg ttttttatat tttcaggaag ttaatattca ggaggaatct
    50641 attaatgaaa atgtcaaaaa tgtttatgcc gacattaaaa gaaatcccag cagatgctga
    50701 gattacaagc catcaattaa tggttaggtc aggcatgata aaaaaaatga cttcaggtgt
    50761 ttataaccaa ttaccaatgg ggttaagagt gttcaaaaaa attgaacaaa taattagaga
    50821 agaattaaat aaaaaagatt gtcaagaaat actttgtgca gccttacttc cttctgaatt
    50881 atggaaagag tctggaaggt ggactgctat gggtgaagaa atgtttagac taaaagacag
    50941 aacagaaaga gagtactgtc ttgggccaac acatgaagaa gcattcactg atataataag
    51001 acaagaaatt acttcatata aacaattgcc actgaacttg taccaaatac aagtaaaata
    51061 tagagacgag agaagaccaa gatttggtgt tatgagaact aagactttta caatgaagga
    51121 tgcatatagt tttgatgtag atgataaagg tttagataag tcatatcaag atatgtttga
    51181 tgcatatgta agtatatttg atagatgtgg attggagaat agccctgtac aggcagattc
    51241 aggagctata ggaggttcta catcagccga atttatggtt aagtcagaag ttggtgaaga
    51301 tgaagttgta ttttgtagtg gatgtgatta tgcagccaat gtagaaagag cagaatcttg
    51361 taatctagca tcacaaaaag aagaaatgaa agaattagaa gaggttcata caccaggagc
    51421 agctacaata aaagagttag aagaattttt aaaaacttct ccagataagt ttgctaaaac
    51481 tttagtatat gaggcagatg gaaaaacagt tgtagtcgtt gtaagaggag atagagaagt
    51541 taatgaaatc aaagtatcta atgctatagg ttctgttata gaatttgctc ttgctacaga
    51601 tgatgtagtg agaaaagtaa ctaatgcaga agtaggattt gcaggtccaa taggtataaa
    51661 tgctgactat gtatttatag ataaagaaat tgttgaacaa agaaacatag ttgttggggc
    51721 taataaaact gagtaccata taaaaaatgc taactatgga agagattttg aaggaatagt
    51781 tggagatttt agaaatgttc aagaaggcga ccagtgtata gtatgtggta agccacttga
    51841 aatagctaga ggtgttgaag taggacatat atttaaacta ggaactaagt actcagaatc
    51901 tatgaatgca aacttcatag acaaagatgg aaaatcaaag cctatagtaa tgggatgtta
    51961 tggaataggt gttgaaagaa cagcagcagc tataattgaa caacataatg atgagaaagg
    52021 tataatatgg cctttgtcag tagctccata ccatgttgtg atagttccag caaatatgaa
    52081 aaatgaagaa caaatatcaa tagctgaaaa tatatataat gatttgcaag ctatgggtgt
    52141 agaagtttta ttagatgaca gagatgaaag aataggtgtc aaattcaatg attcagaatt
    52201 aataggaata cctatgagga ttacagtagg taaaaatatt aatgaaggta aagtagaatt
    52261 taaacttaga cataaagaag ataaagagat aattgacata gaagaaataa atgaaaaagt
    52321 aaaggcagag tttatcagga ataatgttag gttaggataa taacttaaat aagatattgt
    52381 atagataatc aaagttgtac tttatagaca ccattttatc tatatataag gatataaaat
    52441 ggtgtttttt tataaaacaa ataaatgagg aggtaattga aatggcaaaa aatgaaaaac
    52501 aatttgttga agaaataaca aagatggaag atgattttcc acaatggtac acagatgtaa
    52561 taactaagac tgacttagtt gattatgcac cagtaaaagg ttttatggtt gtaaagccat
    52621 atggatatgc attatgggaa aagatgcaag aatttatgga taaaaaattt aaagaaactg
    52681 gacataaaaa ctgttatttt ccattattaa taccagagag tttattaaac aaagaagcag
    52741 aacacgttga aggttttgct ccagaagttg catgggtaac acatggagga aataaaaaat
    52801 tagaagaaag attatgtgta agacctactt cagagactat aatatgtaca atgtatgcga
    52861 aatggttaaa atcatataga gaattaccat atctttataa tcaatggtgc tcagttgtta
    52921 gatgggaaaa atcaactaga cctttcttaa gaacatctga gtttttatgg caagaaggtc
    52981 atacattaca tgaaacagca gaagaagcac aagaagaaac aatacaacaa ttagaagtat
    53041 ataaagcgtt atgtgaagaa ttacttgcaa tgccagttgt agctggtcaa aaaagtgaaa
    53101 gtgagaaatt tgcaggtggt gaaagaacat atactataga agctatgatg catgatggta
    53161 aagcattaca atcagggaca agtcacttcc taggtcaaca ctttactaag gcttttgata
    53221 ttacttttgc agatagagaa ggaaatttag ctaatccata ccatacatct tggggagcat
    53281 ctacaagact tataggtgga cttataatga ctcatagtga taacagaggt ttagtattac
    53341 caccaagagt agctccaatt caagttgtta tagttcctat agctgctaaa aaaggaaatg
    53401 taatggagac tgttgataaa atatatgctg atttaaaaac taagggagta gccgtagaag
    53461 ttgatgatag agataactat acaactggat ggaaatttaa tgagtgggaa atgaaaggtg
    53521 ctccagtaag agttgaaatt ggaccaaaag atatagaaaa taatcaagct atggtattta
    53581 gaagagatac tttagaaaag gattctatgc ctttagaagg attagctgat gctatatgtg
    53641 atttatttga tgttatacac aatgatatgt ttgaaaaagc aagaaaacat agagaagaca
    53701 atacatctat agttgaaaat atggatgagt ttagaaaagc tttagaagaa aagccaggat
    53761 tcataaagac tatgtggtgt ggagatgcag aatgtgaagc taagattaag gaagaaacag
    53821 gagcaactat aagatgctta ccttttgaac aagaaaactt aggtcacaag tgtgtgtact
    53881 gtggaaaaga agcagatagt atggtagtaa tggcaaaagc ttactaaaat acatttaaat
    53941 aaaaactggg gtagaaagta tttaatactt tatcccagtt tttttgtttt ttatgagtaa
    54001 taaaatgtat ttgtaactat gtcataagat aagtcataga tacttatatt aaaaagaata
    54061 taatagagaa ctatttttaa taattacata ttaatgaaag caaaatattt ttgttatata
    54121 gattttcaat aaagcaaaca ataaatataa atgaatatta gtaaggagtg cttttaattt
    54181 ttagaaaata aatgacttaa aagaaagatt tatgaaatta aaactaggat tttctgatat
    54241 aataaacatg tgtattttta aataaaaagg atattattat tagatttgat taaaaataaa
    54301 cttgcaaaac aatgtactag tttaatatta tagttatatt gaataaaata tcaggaggaa
    54361 gtaaaaatga atgtaagagt aaggtttgca ccaagtccaa caggatttgt acatataggt
    54421 agtttgagaa ctgctttata taactattta tttgcaaaaa aaatgggtgg agaatatata
    54481 ttaagagtgg aagatacaga ccaaagtaga ttagtagaag gtgctataga gaatatgctt
    54541 aatgctatga aatgggctgg tgtcaaccat agtgaaggtg ttatattaga tgatagtgga
    54601 aaagtagtac aaaaaggaga atatggtcca tatattcaat ctcaaagatt agatatatac
    54661 caagaatata taaagcaact tttagatagt ggaaaagctt attattgttt ctgtacaaaa
    54721 gaaagactgg atgaagtaag agatgctcaa agagcagcag gagaaacagc taaatatgat
    54781 ggtcattgta agaatctttc taaagaagaa gtagaagcga atataaaagc aggaatacca
    54841 tatgttataa gattaagatt gccagagaat catacaatta agtttacaga cttagttaga
    54901 ggagatatgg aatttaatac taatgaccta gatgaccaag tattaatgaa aacagatgga
    54961 tttccaactt atcattttgc agtagttgtt gatgactatt tgatgaaaat tacacatgtt
    55021 ataagaggag aagaatgggt atcatctact ccaaaacatg tttatttata tgaagctttt
    55081 ggatgggaag cacctgtatt tgtgcattta ccaaatatac ttaataaaga aaagaaaaaa
    55141 ttaagtaaaa gacaaggtga tgtagctgtt gaagacttta agacaaaagg atatttacca
    55201 gagggattag taaactatgt agcattagtt ggttggtctc ctgaagataa tcaagaattg
    55261 tttacaatgg aggaattaga aaaagccttc tctgtagaaa gagtatctaa aagtggtgga
    55321 gtatttgata ctgaaaaatt aaattgggtt aatcagcact atataaaaga tggtgatgat
    55381 gcttatctaa cagacttagc aataccattt ttagtagaag atggatttat aacagaagaa
    55441 gaagctacta ataaatatga tttcttaaag agtatgatat cagtacttaa agagaaatta
    55501 caatatgtaa aagaaattac tgaacatgca agtatatttt ttggagataa aatagaggta
    55561 gaaactgaag agggaagaga gttcttaaga ttagaacata tacctacttt aatagatgct
    55621 ttgagagaaa aaatagaaaa aacagaggta ttaaatgctg agtttgttca agctatgctt
    55681 aaagaaatac aaaaagagta caaaataaaa gggaaaaatt tatttatggg ctcaagaata
    55741 attttgacag ggcaaatgca tggtccagat ttaccaaaag taatggaagt tttaggaaaa
    55801 gaaacttgct taaatagaat agcttatgta aaaaataata tattatagtc ataaaagtcc
    55861 gctaatttag aagcggactt tttttgtgtt agtatttggt gaaaatatct gcttaagaaa
    55921 atgaaggtat ttaaggtaat aaatttaata tataaaaagc aaagcaaaaa tataaataaa
    55981 atatgaataa taaactaata aaatattaag catagaagaa gggaatctat tattttagtt
    56041 tgatttaatt attaaggggg taattactat gtatagtaat attgatgatg ttaaaaagga
    56101 attaaaggag ctatgcttag aatatgttac aattttagaa aaattaaaag atgaaaaaat
    56161 gattacagaa gaaacttttg aaaagtgctc ttctcaaaaa aaaatatttt tagaagaaca
    56221 atagaggatg gtaatagtat tattattatg tagtttgaaa gatagtacga aaaatgatag
    56281 taatactatt atttattgca attttcaact ataatataat atataatgtt aagaagataa
    56341 tttataaaaa tacagtgagg tgatgagtgt gaaagtttat aatacattga ctagaacaaa
    56401 agaagaattt gttccattag aagaaggaaa ggtaaaaatg tatgtgtgtg gtccaacagt
    56461 ttataactat atacacatag gtaatgcaag accatttata atatttgata ctctaagaag
    56521 gtatttagag tatagaggat acgatgtaac ttatgtacaa aactttacag atgttgatga
    56581 taagattata aatagaagtc atgaggaggg aatatctcct gaagaagttg cggctaagta
    56641 tataaaagaa tattttgtag actgtgatgg gctagggata aaaagagcta ctgttcatcc
    56701 tcaggttaca gataatatac aacagattat agagtttata aaagagttgg aagataaggg
    56761 atatgcatat gcagtaaatg gtgatgtata ttttgataca aataaatttg aaggttatgg
    56821 aaaactttca ggtcaaaaac aagaagattt agaggcaggg gctagaattg aggttaatga
    56881 ccaaaaaaga catcctatgg actttgttct ttggaaggca aaaaaagaag gtgagccagg
    56941 atgggatagc ccttggggag aaggtagacc aggatggcat atagaatgtt ctgttatgtc
    57001 taagagatat ttaggtgaaa caattgatat acatgcaggt gggcaagatt taacattccc
    57061 acatcatgaa aatgaaatag ctcaaagtga agctagaagt ggaaaaatat tctcaaaata
    57121 ctggatgcat aatggatata taaatataaa tgatgaaaag atgagtaaat caaaaggtaa
    57181 tttctttaca gtaagagata tatcaaaatt atatgattta gagatagtaa gatttttcat
    57241 gttatcagca cattatagaa atccagtaaa ctttagtgat gagatgttaa atcaagctaa
    57301 agcaggtctt gaaagactat ataatactaa agaaaaatta gaatttacgc taagtaattt
    57361 agtagaatct cctttaacag aaaaagaagt agagcttgtt aaagaattag atgactttag
    57421 acaaaaattc atagatgcta tggatgatga tgttaataca gcagacgcag taagtgtaat
    57481 atttgaatta gctaagttaa taaattctaa tgtagatgaa aattcttctt tggagtttgc
    57541 taaaaaatgc ttagatgaat ttaatgaact tacaggagta ttaaatattg taaataagaa
    57601 aaaggatact gtattagata aagacattga agaattgata caaaaaagaa ctgatgcaaa
    57661 gaaaaataaa gagtttcaat tagctgatga tattagacaa caattactag atatgggaat
    57721 tgtattagaa gacacaagac aaggtgttaa gtggaaaagg atataaaaat ggaaaaaact
    57781 gaattagtta ctatgtcacc attagtttta gcatacttag gtgatacagt gtatgagaca
    57841 tatataagag aatatttaat tagacagaat acacaaagaa aagttaatga tttgcataaa
    57901 ctagctataa agtatgtaaa agctaaagct caggcaacta taattcatga aattgaaatt
    57961 gagttaactg aagaagagag taaaatttat aaaaggggaa gaaatcaaaa gtcaaatact
    58021 tctcctaaga atgcagatat tatagactac aaacatgcaa ctgggtttga ggcattagtg
    58081 ggatatttgt atttaaataa tgaaatagag agacttcaat atattatcaa taaaggaatc
    58141 aaaattattg aaagagatat gtaaatatta aagagtgttg tcttaataat aagtaataag
    58201 gcaacacttt ttttataact aatgtataat aaatatatag aaattagtaa tgagaaagga
    58261 taagaaagtt atgaaagtaa aattaatatc acatacacct gaaccagaaa aagtaatagc
    58321 tatggcagct aagctatgtt attcaccagt tgggacagac gaaatagaaa aagatttcta
    58381 aaatatatta aagagcattc ctataacaaa tactatggcg gttggaatct atgagaagtt
    58441 atagatatag aattatgtaa attatttgga aaaaacaggt ttgaaaatta gccatgttgg
    58501 tatttctagg ttacttaagg aagatcaaac ataaataagc tgagttgtct taggtgcact
    58561 atgtacaaca ttagaatgtg aagttgaaga tttattactt caaacgaacg atggtactaa
    58621 gaaaaacaat gatgaaatga ttaattaata ataaaggtaa ggaggtagta tatgagttta
    58681 gcagataaaa tttttattga tatgtgtaat aatattcttg aaaatggagt atcatcagat
    58741 ggagaggtag ttagagcaaa atgggatgat ggccagcctg ctcatacaat aaagaagttt
    58801 tgtgtagtaa ataggtatga tctttcaaaa gaattcccaa tacttacact aagaccaaca
    58861 aacttaaaag cagcaataga tgaattactt tggatatggc aaaaaaaatc taataatata
    58921 aatgatttga atagccgtat ttgggatagc tgggctgatg aaacaggttc aatagggaaa
    58981 gcttatggat accaaattgg acagaaacat aaatacaaag aaggagaatt tgaccaaata
    59041 gatagagtga tttatgactt gaaatataat ccttatagta ggagaattat aactaatata
    59101 tataatcata atgatttaaa tgaaatgaat ttatatcctt gtgcatatag tatgacattt
    59161 aatgtaactg gaaatagatt gaatgcaata ttaaatcaaa gatcaaatga tattttggta
    59221 gctaataatt ggaatgtaac tcagtatgca attttagttc acatgttagc acaaataagt
    59281 gatttggaag taggcgaatt ggttcatgtt atagcggatg cacatatata tgatagacat
    59341 attcctctag ttaaagagct tattaataga gaatcttatg atgcaccaaa attaataata
    59401 aatccaaaca taaagaattt ctatgacttt agagttgagg actttacatt agaaaattat
    59461 aaaagtgggc cacaaatcaa aaagattcct gtggcaattt agaaggagtt atttatgaaa
    59521 gttatagtag ctgtagattt aaattgggga attggttatg gtggaaagtt actacagtcg
    59581 attcctgaag atatgaaatt ttttaaagaa aaaactgtag gtaatgtagt tgtaatgggg
    59641 agagaaacct ttaaatcatt accaggaaaa aaagcattaa aagatagagt gaatatagtc
    59701 ttgactagaa gtaaatcttt taatgatgat agattgattg tttgtaattc tatagatgaa
    59761 actcttaagg aactacaaaa atatgaaaat gataaaattt ttataatagg tggagaaaca
    59821 gtatataaac aatttttacc ttattgtaat gaactatatg taacaaaaat acagaagaca
    59881 tatgaggcgg atagattttt ccctaatata gatattatgt caaactggga tttgattaaa
    59941 gaaagtgaag taaaagaata taacaatata agatatgttt tttcaacata tataaataat
    60001 agtttatagg tttgtatagg agagaaattt aatttatgaa aaaggatata tttgtaaata
    60061 gagtttctaa tgctttagaa aatgatagag gcgcagtttt tgtaggtagt ggtatatcgt
    60121 ttagttcgac tgaagttgat tggtttaaat tactggagcc attagtaaat gaacttgata
    60181 taacattaga caataaaagt gatgatttac ctatgattgc tcagtacata gtaaataatt
    60241 attcaggaaa tagagggcct ttagtaaacg aaatatcaaa aacatttaat agaaagttta
    60301 aactaaacaa gtatcataag tatttagcaa caactaaact ttcaacaata tggacaacta
    60361 attatgatat gttgttagaa gatgcatttt atgattttga tacatcggtt aaagtaagtg
    60421 atgattctat tacaaggaat agactagaat atgatgtgga aattataaaa atgcatggat
    60481 gtatttctag atccgattgg aatgaaatag ttataactca agaagattat gatgattttt
    60541 caataaataa accagctata tctcaaagat taagtgaaga tttaataaat aagtcttttc
    60601 tatttattgg atatagctat agagatccaa atataagaaa tataatgata aatgctagaa
    60661 ggctaagtaa aaaacatact caacaacatt atataatttt aaaaaagtta aaagatagtg
    60721 atagtaatat tacgcaagat gatgtagtaa gacaagaact ttggtgcaat gatttaaaac
    60781 gtttgggaat ttcaacttta tttatagatg aatactcaga gttagaggaa cttttaagag
    60841 agataagtca aaaatcaaga ggtaaaacag tatatgtgac aggttctcat aaagaaagca
    60901 atatgaatgc aagaaaatta ggaaccaagc tagcaaaaca taaagatatt actttactaa
    60961 gtggtcaatc atctggtatt ggctcagagg tagttgctgc gtttactgaa gagtgtatta
    61021 agagtaaaga agatattata aatagaatga aaatatttcc taatccatat tcagctgatg
    61081 aaaaatactc tgatgatatt actttaatac cagatttaaa gaaatgtaga actaaaatgt
    61141 tgaattcaac acaagttgta gttgtattta gtggaggtat aggtactgaa gcagagatag
    61201 atgttgctat aaaacaaaac tgtaatataa ttcctgttat aacatgtaaa gaggatagag
    61261 agaatccagc tataaaaaaa atcttaaact atagtgataa tatgaaaaat atagaaataa
    61321 aagatgctac ctattataat aaaatagtag atgaagatgg tgttgtaaca atggaggaaa
    61381 tttatatatg catatcaaaa ataatgaatt aaaacatgac attattaaat tgaaaaaaag
    61441 aaatggattt attgaagttg gaaaagataa aaaaacgttg gtaaactgtt taattggagc
    61501 gaatgaatta gaggattaca aatatgaaat aaaaaaaata gattgtatac ttaatatatc
    61561 agaaaaacca gatatagttt cagatttaag tactaaaaga agaaaaataa gtgaatctat
    61621 atggtataag atagttaaag atacaccttt cgtatctgct acactgccca tatacttagt
    61681 tagtaaaaat aataataaaa tagatagtaa tgaattatta gatataatta ttgagcagat
    61741 ggaaaatgga gttgggatga taacgattca tccaacagca aataaacaaa tttttgagga
    61801 ttctaagaat agaatggtac ctgtcacatc tagaggtgga ggtatggtaa tacaagattt
    61861 aatctcaaaa aatttttcag aagataatgt gtatttgaaa atattgccag aaattataaa
    61921 gtatgctcaa aaacataatg tttgtatcag tctaggtgca acatttagat caggaaacat
    61981 atttgattct aatgatagag cacagataac agaaattaag agtcaaatag aattagctaa
    62041 gaggatatat aaaagtaatg tcggggttat tattgaatca ccaggtcatt caagaccaag
    62101 tgacataatt aaaatatcgt caatattaaa aaaagaaggt ttcccaatta tgccactagg
    62161 accaatacca acagatattg caataggaat ggatcatatc gctgcagcta ttggtgctac
    62221 aattatgggt atagaaggat gtgcaaatat aatatcaact gtcacacgag aagaacatac
    62281 tggagggaga ccttctattg aatccacagt tgaatctata agaacagcaa aaatagctgc
    62341 tcatattatt gatatacatc aaatgaatga tattaactta gatatgagtg tatctaagtc
    62401 tagagctaaa tctaacacct gtatatttgg gaaagataca agatattgtg acagatgtag
    62461 agaactgtgt cctttgtgca ttagataaaa attatattca agttaattca ataaaaaaga
    62521 ttggataaaa atacatcgta tagctagttt ttcacaacag agtcaaagat atgtcaaatt
    62581 agagcaattt gaatacataa ttcctccaga aatagaaaaa atagaaaaag caaaagagtt
    62641 atttataaat tcaatgaaaa aataccaaga ggattatgat aaattagtag agatactatt
    62701 tgaaaatcat tataacaaac taataaaagg tggtaaaaac gagaaaacat caaaaagaca
    62761 agttgagaag aaagcaatag aagatgctag atacgtattt ccaaatgcgt gtgaaacaaa
    62821 gatggtattt actataaatg ctagaagttt gtttaatttc tttgaacata gatgttatga
    62881 aaatgggagt tgtctagaag gaactatgac ttgtggagat atagttcaag ttagaaaaaa
    62941 atttaagaac ttgtaggagg tgaataattt ggcgagtata gaaggaagaa atccggtaat
    63001 agaagcaata aaaagtgaca gagaaataga taaaatatta attgcaaatt cagctaaaga
    63061 aggttcaatt aagaaaataa taggaatggc taaagataag aatataatta ttcagtatgt
    63121 tgataaacat aagctagatg aagtaagtac aagtcattca catcaaggtg taatagctta
    63181 tgcaagtgag tataagtatt atgaattaga tgagctaata gatttagcaa agaataaaga
    63241 tgaagaccca tttttcatta tattagatga gataacagac ccacataatt tgggttcaat
    63301 aataaggaca gcagatgcag taggagcaca tggagttatc attccaaaaa gaaggtctgt
    63361 acacataact cctgtagttg ccaaagcatc tgctggagca gtagaatata tgccagtttg
    63421 caaggtaacc aatatagtta atacaataaa aagactcaaa gaagaagggc tgtggattgc
    63481 tgcagctgat atggatggag aaacttttta taaacaaaat cttactggtc ctcttggtgt
    63541 agtaattgga agtgaaggtt ttggaatatc tagattggtt aaacaaaact gtgactttat
    63601 agtaaaaatg ccaatgatag gtaatgttac atctttaaat gcatctgtag caggtggaat
    63661 acttttatat gagatattca gacaaagact ggataaaagt aaataggcat gaaaaaaaat
    63721 ataaatcatt acttaataat agatggctac aatattataa atgcgtggga caatttgaaa
    63781 gaactggcta aagaggactt agaagattct cgagaaaagc ttattgatga cattatagaa
    63841 ttttctgagt ttatgggtta taagactata atagtttttg atgcttataa tgttaaaaat
    63901 tctcgagaaa aagttgaaaa aagaaaacac ataacaatag tttatacaag agaacaccaa
    63961 acagcagata gttatataga gaaatttata acttctttat caaagtatga tgatgtaaaa
    64021 gtagccacta atgactatgc agaacagcaa atgattttag gaaaaggtgc tactagaatg
    64081 tctgcaagag aattaaagtt agagttagat cgttccaaga ataaaatgaa ggaaaaaaat
    64141 atcggtttga ggaaaaaaat tcagcgaaat tggttggagg aaaggttaga taaagaaaca
    64201 ttgtcgaaac ttgagaacat tcgtagaaag cgttgaaatt actaggttct ttagactata
    64261 atatacctat aaatttgttt tatatatggg ggagatttac atgttggtag caaaagaaaa
    64321 aagttatgag ttagtggaca attgtcagca ggatgagtat aacattgtat taagagcaag
    64381 tgaaggggat aaaatagcac tagagtacat tattactaaa tacagaaatt ttgtaaaggc
    64441 aaaagcaaag tcatattttt taattggagc agataaagaa gatataatac aagaaggaat
    64501 gataggcctt tataaggcag ttagagattt tgatggaagt aagaccaatt catttaaatg
    64561 ttttgcggag atatgtataa caagacaaat aataactgct ataaaaactg caacaagaca
    64621 aaaacatata cctttaaatt catatgtttc tttgaataaa ccaatatatg atgaagagtc
    64681 agacagaaca cttttagata ttatagctac aagtatagta acagatccag aagagcttat
    64741 aattagtaaa gaagagttaa aaaatataga atcaaaaatg aacgaattgt taagtgactt
    64801 ggaattagaa gtactagaat tatatttaaa tggaaagtca taccagttta tagcggacaa
    64861 gcttaaaaga gatgtaaagt caatagataa cgctttacaa agggttaaga gaaaattaga
    64921 aaaacatctt gaaaacagaa atgattaata gtaaaatact taccataagc ttattgtgag
    64981 caaaattaat ttatatcgaa agataaggga ggaaattcaa aatggctaaa gctaaatacg
    65041 aaagaacaaa acctcatgtt aatataggga caataggaca cgtagaccac ggtaaaacta
    65101 cattaacagc agcaataaca aaaacattat atgacagata tcaattagga gaagcagtag
    65161 atttcgcaaa catagataaa gctccagaag aaagagaaag aggaatcaca atatcaacag
    65221 cacacgttga gtatgaaaca ccaaatagac actatgcaca cgttgactgc ccaggacatg
    65281 ctgactacgt taagaacatg ataacaggag cagcacaaat ggacggagca atattagttt
    65341 gttcagcaac agatggacca atgccacaaa caagagagca tatactatta tcaagacaag
    65401 ttggagtacc ata
//
LOCUS       DAMJVX010000004        61852 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMJVX010000004 DAMJVX010000000
VERSION     DAMJVX010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 61852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61852
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.004"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(115..1716)
                     /locus_tag="PC407_000205"
     CDS             complement(115..1716)
                     /locus_tag="PC407_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2495688.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDDLRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDNKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGIIKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(1827..2504)
                     /locus_tag="PC407_000206"
     CDS             complement(1827..2504)
                     /locus_tag="PC407_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495689.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(2517..3212)
                     /locus_tag="PC407_000207"
     CDS             complement(2517..3212)
                     /locus_tag="PC407_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF2495690.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(3264..4463)
                     /locus_tag="PC407_000208"
     CDS             complement(3264..4463)
                     /locus_tag="PC407_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HDF2495691.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDICRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARNLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(4724..6769)
                     /locus_tag="PC407_000209"
     CDS             complement(4724..6769)
                     /locus_tag="PC407_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2495692.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNILIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILNSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(7180..7989)
                     /locus_tag="PC407_000210"
     CDS             complement(7180..7989)
                     /locus_tag="PC407_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2495693.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTFVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(8073..8606)
                     /locus_tag="PC407_000211"
     CDS             complement(8073..8606)
                     /locus_tag="PC407_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2495694.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGLNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(8733..10808)
                     /locus_tag="PC407_000212"
     CDS             complement(8733..10808)
                     /locus_tag="PC407_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2495695.1"
                     /translation="MLKGLSFRNARRQAGKYLIYWCSLIGSVALIYAFNALIFSDVMQ
                     ELSSILNNGGSDEFSYMIIIFSFIITFILGWFVSYMMNFILKKRSREISTYMILGVDK
                     KQIVKMLFIENLLLGASALIVGWILGIFVSKVLESIVINMFHGKYSLSPDFSLKAAGM
                     TFLYFCIIYLIAQLKSNKKLVKMKLINLLNYDRYNENVLIHQTKTKSIMFCVSVLCGG
                     MGIFFFSIPSGRLTDVFLGLLLIVFCLIVLFMGFGSIAQNMFEKGMNWKYKKSHLFLY
                     RLLASKINGISIIMGIIATLFTVSIACIGIANSFYSVMNKSVDLQQFDVSIFHPNESY
                     DFSSYEGYLSETKGVKKDYSYTLYTEEKTSFMKVRNNILSKYLKDIGRDLQYKDYTFA
                     ENQFDTFMKYSDYNILRNMLGLNPIKMDKNQFIIHCMPYLADAYKNYIGEKYKLTISD
                     KDLSIAGIYIENFSQYGGYGNGQEYIIVIPDYLIDNMKPLYSLYVAITSEPLDSAYFT
                     EFQKKFDTLENLNSGVVVAGNQGFVSKLRYDNKDYISGKYAMQSTGQAIILILPLFYL
                     ALIICIIGTVILSIQLLSENNIIIKHYGMLRTLGMIDSEVIKILKKHVCLYFSIPLIP
                     AVIFGGILVSVFSYNMFNISFDAPVFFNIQIPILHAVAITLINFVVIYCIYVFITYVA
                     LRKEILHIK"
     gene            complement(10811..11557)
                     /locus_tag="PC407_000213"
     CDS             complement(10811..11557)
                     /locus_tag="PC407_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2495696.1"
                     /translation="MLLRVENIEKYYGNQKILTKALNDVSFQINTGEFVSIMGTSGSG
                     KTTLLNCIATIDTVSAGKIYLENQDITELSEENLTVFRRDNLGFVFQDSNLLDTLTLE
                     ENIALPLTIKKVSPVEITNSVYNIATQLKIEEILSKFPYEVSGGQRQRCAVARAVVTE
                     PKLILADEPTGALDSNSSKTLMELLETINTILEATILLVTHDAISASYTERILFLRDG
                     KIFNELRKGEKSRLTFYHEILDALSLMGGD"
     gene            complement(11571..11966)
                     /locus_tag="PC407_000214"
     CDS             complement(11571..11966)
                     /locus_tag="PC407_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HDF2495697.1"
                     /translation="MKKRILTILCVVLVLFISITGLDSMGFFSRSIIPLRSEYASFIR
                     KNGTITEVKFTGPFNHNNIDLNKNVEDTNKNGDDNELTIKYDIEVKKSLISSNEPTIQ
                     IQDSKESIYVYILNFSDKTIIIKNGKIVD"
     gene            complement(12079..13101)
                     /locus_tag="PC407_000215"
     CDS             complement(12079..13101)
                     /locus_tag="PC407_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2495698.1"
                     /translation="MRFCNFLQDKVLVILLNICCAISLVLYLHLIGIGQGELTLLIAA
                     WVAILFCWICFCYQKQKKEIIKLRRIVDELDKKYLFIEMIEKPTSNIEREYYNIIRLS
                     LKSMTDHVSQIHREMYEYRQFIEQWVHEIKHPVTTSELICENYKNEHTRKIRFQMEEI
                     ERHIERALYYARLGHVEKDYLISEITLKSIVEEVLAKNKLLLIQNNIHINIQDLEYSV
                     YTDKKWIVFILNQIIINSVQYKSDSAEISFFAIKEDKSVKFTIKDNGIGIKESEIYRI
                     FELGFTGSNGRTTNNSTGIGLYLCYELSKKMGIDIDVSSSLGEYTAITLNFPINTAIN
                     MQKQCN"
     gene            complement(13098..13769)
                     /locus_tag="PC407_000216"
     CDS             complement(13098..13769)
                     /locus_tag="PC407_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2495699.1"
                     /translation="MILTNILIIEDDVFICKELMQFFENNGYSTQTLDTNNCDVLQVI
                     QESTPDLILLDINLPNIDGFSLCRQIRNCSTIPIIFITARDSAIDELNGLTMGGDDYI
                     TKPYNLPLLLARVKNLLKRVDSSDKQKIKYREIILNLISATVEYQNAVIELSRTELKI
                     MYYLFQNVGSIVPRIDLVEYLWDNHIHIDDNALSVNITRLREKLRGIGITDLIITKRG
                     MGYRI"
     gene            complement(13855..15204)
                     /locus_tag="PC407_000217"
     CDS             complement(13855..15204)
                     /locus_tag="PC407_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2495700.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNRANSIEEIKPCEISKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIDKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEVIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(15216..16598)
                     /locus_tag="PC407_000218"
     CDS             complement(15216..16598)
                     /locus_tag="PC407_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2495701.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            16842..18056
                     /locus_tag="PC407_000219"
     CDS             16842..18056
                     /locus_tag="PC407_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2495702.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIKLYGNKCDYAKRDTLLYTANKLEIKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            18233..20146
                     /locus_tag="PC407_000220"
     CDS             18233..20146
                     /locus_tag="PC407_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2495703.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDNIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKVSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            20417..21391
                     /locus_tag="PC407_000221"
     CDS             20417..21391
                     /locus_tag="PC407_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2495704.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            21412..21687
                     /locus_tag="PC407_000222"
     CDS             21412..21687
                     /locus_tag="PC407_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2495705.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            21700..22863
                     /locus_tag="PC407_000223"
     CDS             21700..22863
                     /locus_tag="PC407_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2495706.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGTEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            23200..25480
                     /locus_tag="PC407_000224"
                     /pseudo
     CDS             23200..25480
                     /locus_tag="PC407_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(25575..26915)
                     /locus_tag="PC407_000225"
     CDS             complement(25575..26915)
                     /locus_tag="PC407_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2495707.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILIGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVTYYFIR
                     GKSTLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     IVQVFPEVFVGIFNKDSDLMDIAVRGISINLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(26927..27709)
                     /locus_tag="PC407_000226"
     CDS             complement(26927..27709)
                     /locus_tag="PC407_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2495708.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(27724..28746)
                     /locus_tag="PC407_000227"
     CDS             complement(27724..28746)
                     /locus_tag="PC407_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2495709.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(28752..29765)
                     /locus_tag="PC407_000228"
     CDS             complement(28752..29765)
                     /locus_tag="PC407_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2495710.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(29893..30831)
                     /locus_tag="PC407_000229"
     CDS             complement(29893..30831)
                     /locus_tag="PC407_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2495711.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWSQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            31101..31565
                     /locus_tag="PC407_000230"
     CDS             31101..31565
                     /locus_tag="PC407_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495712.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTGKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(31677..31994)
                     /locus_tag="PC407_000231"
     CDS             complement(31677..31994)
                     /locus_tag="PC407_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2495713.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(32007..32738)
                     /gene="chbG"
                     /locus_tag="PC407_000232"
     CDS             complement(32007..32738)
                     /gene="chbG"
                     /locus_tag="PC407_000232"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDF2495714.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVVEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(32764..34074)
                     /locus_tag="PC407_000233"
     CDS             complement(32764..34074)
                     /locus_tag="PC407_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2495715.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNNGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLVAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(34151..35464)
                     /locus_tag="PC407_000234"
     CDS             complement(34151..35464)
                     /locus_tag="PC407_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2495716.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(35486..35797)
                     /locus_tag="PC407_000235"
     CDS             complement(35486..35797)
                     /locus_tag="PC407_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2495717.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(35997..36701)
                     /locus_tag="PC407_000236"
     CDS             complement(35997..36701)
                     /locus_tag="PC407_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2495718.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(36796..38157)
                     /locus_tag="PC407_000237"
     CDS             complement(36796..38157)
                     /locus_tag="PC407_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2495719.1"
                     /translation="MKDRINLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAIYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     MSE"
     gene            38656..39801
                     /locus_tag="PC407_000238"
     CDS             38656..39801
                     /locus_tag="PC407_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2495720.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQDVINKKYIVYRIGGDEYIIILKNLSHKEINSIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKKKINK"
     gene            complement(39891..41609)
                     /locus_tag="PC407_000239"
     CDS             complement(39891..41609)
                     /locus_tag="PC407_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2495721.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(41614..42603)
                     /locus_tag="PC407_000240"
     CDS             complement(41614..42603)
                     /locus_tag="PC407_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2495722.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(42699..43748)
                     /locus_tag="PC407_000241"
     CDS             complement(42699..43748)
                     /locus_tag="PC407_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2495723.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTYNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(44025..44693)
                     /locus_tag="PC407_000242"
     CDS             complement(44025..44693)
                     /locus_tag="PC407_000242"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDF2495724.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(44699..46072)
                     /locus_tag="PC407_000243"
     CDS             complement(44699..46072)
                     /locus_tag="PC407_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDF2495725.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(46069..47847)
                     /locus_tag="PC407_000244"
     CDS             complement(46069..47847)
                     /locus_tag="PC407_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDF2495726.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(47964..48287)
                     /locus_tag="PC407_000245"
     CDS             complement(47964..48287)
                     /locus_tag="PC407_000245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDF2495727.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            complement(48274..49251)
                     /locus_tag="PC407_000246"
     CDS             complement(48274..49251)
                     /locus_tag="PC407_000246"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HDF2495728.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(49266..49829)
                     /locus_tag="PC407_000247"
     CDS             complement(49266..49829)
                     /locus_tag="PC407_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HDF2495729.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(49881..50372)
                     /locus_tag="PC407_000248"
     CDS             complement(49881..50372)
                     /locus_tag="PC407_000248"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDF2495730.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(50375..52300)
                     /locus_tag="PC407_000249"
     CDS             complement(50375..52300)
                     /locus_tag="PC407_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDF2495731.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(52302..52613)
                     /locus_tag="PC407_000250"
     CDS             complement(52302..52613)
                     /locus_tag="PC407_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495732.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VRKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            complement(53243..53518)
                     /locus_tag="PC407_000251"
     CDS             complement(53243..53518)
                     /locus_tag="PC407_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HDF2495733.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DITVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            complement(53633..55042)
                     /locus_tag="PC407_000252"
     CDS             complement(53633..55042)
                     /locus_tag="PC407_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDF2495734.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     SLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGGIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            complement(55412..56074)
                     /locus_tag="PC407_000253"
     CDS             complement(55412..56074)
                     /locus_tag="PC407_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495735.1"
                     /translation="MIDVSIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            complement(56328..58547)
                     /locus_tag="PC407_000254"
     CDS             complement(56328..58547)
                     /locus_tag="PC407_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2495736.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYALNQDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKITISVPLLNNDK
                     VVGVLYCTYNINTLMKLINVSFYENNSISYVVRNDGTIILHPQEDSLSKNIYKLLKQD
                     NDIREINRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSEQIINRAIYAVLFIILIFIAIIFYIIYIKKSNEKEILSLAYEDKVTHIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGKNKRIRKIDSIRNNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LSEWKKQGKKIVPVSINLSRVELYQPDIVKFINKTIQMYNLSSDLIEIEITETVAINE
                     LNILKNVLNELRKYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLNGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(58823..61477)
                     /gene="adhE"
                     /locus_tag="PC407_000255"
                     /gene_synonym="adhC"
     CDS             complement(58823..61477)
                     /gene="adhE"
                     /locus_tag="PC407_000255"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2495737.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYTQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDESADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDNAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRSPGKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEEPKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
BASE COUNT        20673 a        10760 c         7436 g        22983 t
ORIGIN      
        1 atattaatac tataaataaa aacgaagcat gatttttatt taaataaatc ccaaacttcg
       61 tctttactta cttagatatt tatttacttt ctaaatccta ttatttttaa aaccttatct
      121 ttttaatact tcaccacatt tattctcagt aatctctcca ttttctaacg caatttttcc
      181 atttacaatt acatattcaa caccattggc tagtaattgt ggctttgaaa aatcaatatc
      241 ttctgaccta gaataactat agttatccat gtcaataact gctatatcag ctatcaaccc
      301 ttctttaata atacctctat tttttatttt aaattgccta gctggtaatg aagtaactct
      361 tcttacaatc tcttccaatc taacaccaga ctttctgtac atattgaaga aaattggaaa
      421 agctcctctt ctttgtagtt caaaccatga catatcccca cccacactaa acaaacaatc
      481 tgaaccaacc ataacaaatg gattatcaat tattttatta tcatgtagtt tatctgggaa
      541 atcagctcta tacattcctc ctaaacaaaa gataagttct tcgtctccat ctaaaagtaa
      601 atcgagtaat aacttgtcct catctattcc tttttgtgta gctatttcac gtattgattt
      661 cttttccata ttaatatctt tagtgtttag aagtattata ctccccctat ctccaagtat
      721 aaatgcttct tttaatataa gtgttcttcc ctcttcattt ctaagatcat ccattacccc
      781 atctatacct aattcaaata gactagatga tattgccata ataaattgaa ttactctttt
      841 tttcttcata caatgcccag aacttcttgg tactgtgtca accattattt caacaccatt
      901 atacctagcc tttaatatct catcaaatgc ttctatttca actggactta agtgagatac
      961 ttgaacccta gcaccacttt tttgcccaac ctctatggct tcttttactg cattatatct
     1021 tcctatgtaa tctctaatat gagatgtata tataccatca tgctctttaa tctgccttgc
     1081 aatatcaaca agttcatcaa tatcagcata cttacttggt atatatgcca atccagtaga
     1141 tattccaaaa gcaccacttt tcattccatc atttattata tctgtaattt catttttttc
     1201 cttttctgta ggaggtctat ccttagaacc tcccataaca ctccatctta tagtcccatg
     1261 ccccaacaag aatcccatat tgacagaaat accactttta gatataaggt cacaatattc
     1321 tgtaaaatta ttccaacatt tatttttatc tataagatac tttttttctt cttcaagtaa
     1381 tagaccattt ttatacatat attcatatac attttcagaa gaatatggtg ttatagaatg
     1441 tccacaatta ccatttatag ttgttgtaac tccttgtttt agaaactttt caaatttacc
     1501 atctagtatt gctactattt cttcatgaac atgagggtca ataaatccag gtgttataac
     1561 ttttccacaa caatcaatct ctttatttgt attgctattt atcttagaat ctatttttgc
     1621 tattaagttg tctttaattg ctatatctcc ataaaaacca ggattacctg taccatctat
     1681 tataaatcca ttttttaaga ttaaatcata tatcaaaata ttccctcctt aaaaaccaat
     1741 ttttaaataa tcttaatgta atttttatat ttgaaaatca aattattata tttcatattt
     1801 gaaaattcac tattatttca ttgtaattag tcactaagct tatctttata catagctctt
     1861 gcaattaata tagtaccaaa tacacatata agaactacta gagactgtgg tattatacct
     1921 tgctttgata atataatagt caatacagat attacaattg ctactagacc taatttttta
     1981 tcttgtaaaa atacttgtcc aaaaactgac ccaaatagag ctggtaaaat taagtttaat
     2041 ttttctacta cttcagctgg aattttagat aatacgcttc ctcctaaaat tacccctata
     2101 gttagtatac ttatattcac taaaatagat acagctattc ctattgtaga tattatcgag
     2161 ccttcttctg tacctgattc aacttctgct gccttttgag ctgctattga acatggtagc
     2221 ctcatattag atatattacc agaaagaaaa ctcatataag ttcctgggat acctaagatt
     2281 ggtgaataag aaactggttc tattatgtaa tttggagcac tcatactgac aattgccatt
     2341 gtacttgcta ataatgctga tattggaggc ataataccaa atccaaagct caaaattatt
     2401 ccaggaacta atgctgctat aatacctaaa gatagtgtta gtcttccata ccttatcata
     2461 aaaggtaaat attcttcatt gtatatagat tttactttac tcataaaact ccccccttat
     2521 attactgaag ctattatcat tccgctaaac attgatattg taagccccca ctctcttaac
     2581 cattttatat ttttcttctt ttcaagcata cacaatatta ccattatagc cattccactt
     2641 atacatgcaa ttgttccatt atcaaaacgc aacaccctat cagcatttag atatgcaaat
     2701 gaacccaaca ttgctcctag agatattatt ggtataaatg atttcttgcc actagatata
     2761 acattggtta tcttatctag tttatgtcca aatagtaatg tgaatattat ccatcctaaa
     2821 gaacctaata tcatagtcca aactgcattt gtaaatgcaa gttttgtcat ttcaggagaa
     2881 cctaaagtta tacctaatgc atctgttcca aatccagctg acattgattc aaaaattaca
     2941 gaacctataa atgataatct catccatgct ataggcccac ccatagttac tataagcgat
     3001 accattccag caaatatagt cattgaaggc ccaatagctg aaatagcact actcttaaca
     3061 gctgacttta tttgtttatc tgttattccc attgttttac caactttgca agattttata
     3121 gcaaatataa gggattgtac cactactatt gatactgcta ctgtactcgc tatccacaaa
     3181 agtggatgat tagctaattt taaataatcc atttataaaa cctccctata aatcctcaaa
     3241 ttttattaat atttctaata tatctatttc caaacctgtt ttagtactct aagtatattt
     3301 ccacctagta ccttctctat ttcctcatca ctatatccat gctttactaa atatctgaca
     3361 atgttttttg aagtttctgt tgggttctca agacctttta catattctac tttgggatgt
     3421 tcttttgtac ctgttatttt ttttatatca aatttagcag ctagtactga gtgtaatttt
     3481 acatggtcgc catatactgt atctggtcca aaagctacat ggtctattcc tactaaattt
     3541 ttaatatact caaaatgttc cataaatgac tctattgaat gttctttatt cttctctgtt
     3601 attgtagtat gaggtgctgc ttctacacct ataacccctc cttttttagc acatgctatt
     3661 aatacttcat ctggagttag tctctttgtg ttccataaat ttcttgcccc aacatggctt
     3721 aatattattg ggtccttgct atattctatt acatcaagtg tagtttgaac tccaacgtga
     3781 gagcaatcta ttgccattcc aattttattc attctttcaa cagctttttt tccgaaattt
     3841 gtaagacctc catctttctc ttcttttagt cctgaaccta atgcattgga ttcgctgtat
     3901 gttataccca ttaaccttat accaaatcca tacagtatat ctattctatc aagttcattt
     3961 tctattggtg ctgcaccttc taatgttggt atcaatgcta ctttaccttc acttttagct
     4021 ctgcatatat cttctacttt ttcacacttt atgacaaaat cttgatgagc taaatcacat
     4081 agtctcatgc ctaaatctat taatatatca tcccatttcc accctgaatt tgatgttatc
     4141 gtacatacac catccattaa attatcaaat atagcatcat aatatcctct tgataatgct
     4201 tcataagcgc atctttgttt accttcccta ttatattcaa atatatctct ttctaggtgt
     4261 tctggaaata atactggatg ttcatgtaat gatataaata ttttttcatc cactatactt
     4321 ttaaccttag cttcttgaat atcatctaat tcaataaggt actctttaac tcttttatct
     4381 cctttacaca tctcaataga tgaaatatct tttccttttt ctaaataact ataggcttca
     4441 tatcctttgt aagctgactt cataacaaca tcctccaagt tctacaattt tttatatttt
     4501 tcctatgtag caattgatat gccaaaatat aaattttaat tgtataataa atcttgcaaa
     4561 aaataaaaac agtagtaagg ttgatattta ctactgtttt attaaaaata atcttcttag
     4621 attatttatt caatattttt gaattatctt aatcttaaag ataattttat ttatttctat
     4681 ccattatagg gattttttta gggtgttttg cctttttagt cccctatatc actcccttaa
     4741 tttttacata tgatataaag ttctttcctt catcagttat aaaactacct tgttttttta
     4801 ctccagataa aagtaaacca tggctcttta acttatctat tctttttcta atttggtcta
     4861 cacttaaaac tatatctttt tcttttaaaa tctcttgcag tttatttctt cctaataata
     4921 tatttttatt attccaagtt tctatactgt tcaatataca tatactttcc tcaaagaaat
     4981 tagattgttt gaaatctaag ataattgaat cgaaattttc attaactaaa gtattgtttt
     5041 tttcaaatct aaattgttct ggcaaatgct cgtaatctac tctatcttcc tcaactatag
     5101 tatcaatata ataaattaaa tttttaagct ctcttacatt tcctggccat tcatacaatg
     5161 ttaaagcttg cattgattcc tctgtaaaac atttgttgct atttatttca tctaaaaaat
     5221 acttacttat aagaggtata tcttccttac gttctctaag tctaggtatc tctatatgta
     5281 atacattaat cctataatac aaatcttctc taaaagaacc ttctttaatt tttttcttta
     5341 aatctttatt tgttgcagct ataattctaa catctattgg tataatttta cttccgccca
     5401 ttctcataac ttctttttcc tgtaatactc ttagcaatct ctgctgtaca tctaaagata
     5461 tatcacctat ctcatctaaa aaaattgttc ctgtatgcgc tctttcaaat atacccattt
     5521 ttccaccttt tattgctcct gtaaaacttc cttcatcata gccaaaaagt tcactttcaa
     5581 taagtgtatc tgacaaagaa gatagattaa ctgcaacaaa tggcttgttt tttcttagag
     5641 attcattatg tattgcctgg gcaaatattt cttttcctgt tccattttct ccaagtatca
     5701 acacagaaaa gtctgtagtt gctatttttc ttgcaatatt aattttttct tttattattt
     5761 tactctcacc tacaacactc tcaaatgtat actttgatac aaaacctttt gactgaagtt
     5821 tattttgaat cttgtcctca agcacttgaa ttgcacttat atttttaatg cttataatac
     5881 tctttattcg ttcattattt tcataaacat ttactttatt aattatcagt tttttgttat
     5941 ttaaatttac tacttcatca ttaacttcat cttcttgaaa aaatattttt ttgacaacct
     6001 tatcactaaa taagtccata aagttattag atataatttc attttggtct attccaacta
     6061 gattagaaaa tactttgttg caatatataa attttcctaa tttatcaaca gaggcaatac
     6121 cttcatctat aatctccaaa aaactcttca tggttttatt catagttgta taatacctat
     6181 atccacttac tgtatcttca ttgtattttt ccttaataat atgtaattta tctatagata
     6241 tatttagttt tgtaaagatt tctataactg tatttatatc aattacctta tcacctatgt
     6301 caattatttg ctttatgttt tgtggtatgc tgtttctgct tcccgtaata attccaatct
     6361 cacatttaga cttatcacaa cctggataat atggtattag attaatatga attaatccta
     6421 gttttctaat tatcttggca gtttcatctg ctgactcttt ataagcatct ataaccataa
     6481 catcagaatc attttctata cttattatct gatttatatt ttcaatattt atagtacgat
     6541 gtactattac aataagtata tttttatcaa tattattatg tatatattcc tttatatcat
     6601 tgtctgaaca tactacaagc tcatagcttg acagttcaat atttatccta tttacatata
     6661 ccaagttatc tatattacaa aattctccaa atatattttc caattgtttt gatatatcta
     6721 tatttatttt gtcatcagtt gatacaaata aaatattatg ttttttcata gtcccacctc
     6781 ctgtttaacg agtatagaaa gtaataattt ggtctattgt agcacttttt aaaaaagttt
     6841 taaatcttaa aaatcaaatt ttgtaccatt tactattatt ttccataata aaataatgta
     6901 agctatcttt tttcacatat aatttaattt ctaaaacggt tagtttgaca ttattggaat
     6961 ataaacaaaa atttaatata aattcgttaa tatattaata catttttaag aatgacaagg
     7021 aataaattat aacaaatttt aggaatatat aataataggg tctcagaact tagttacaag
     7081 ttctaaaacc ctatttgttt agtgtaaaaa acatcataaa atttagctta aatcttttca
     7141 cataatatta ctctttttct aagtgataat aaaatatatc tattcctcta aaaactgtaa
     7201 aaattcctga ttaaatagtt taagctcatc atagaatata gcatgaccac tatactcaaa
     7261 agtatataaa attgaatctt ctattttttc attcataaaa actgcaaatt catacggaca
     7321 tatttcatct aatttcccat gaaatatacc agtaggcact cttacacatt ttaaatcatt
     7381 aaataattct tcatctctta aagatacagc agttccaata gtgccaatac ctgatgcact
     7441 aaaaccaata tcattaaacc atcttctaaa gctttctggt ggatttgatg caaatacttt
     7501 ttctccaaaa tcggttacca tttctggtct atcagtacat gcttgtgcta tcaatttatt
     7561 tacatcttca cgtgtcatac catatggaaa ttctggaggt ctttgtacaa atgatggtgc
     7621 agcagctgca gctaatacca attttgacac tccatatcca ttaaataaac tcatatacct
     7681 aagtacgatt gctcctccca ttgaaaaacc aacaaaggta aagtctttta atccaatagc
     7741 atgtaccacc ttataaatat cgtctgctaa ttggctataa gtatatcctc ctgatgttgc
     7801 atcagattta ccaaaacctc ttaaatcaat tgaaactgtt ctatatccga gttttggcaa
     7861 tatatttgtt tgatattcaa atatcttatg tcctaagggc caaccatgta taaaaagtac
     7921 agttttttta gcactcggat tcaaatcata tactgctatg tttatatcat tcacatatac
     7981 ataatacata tcaacagcaa ctcccttcaa gaaaatgtat gatggttatt ttatatatat
     8041 gaataaaatg atgaataact aaatttttaa atttatttac aatacttctc tattgccttt
     8101 tcaaataaac ttacatgaag tttttcatct aggattattc tatttaatat agcttttata
     8161 taagggtctt caataagact tatatcttta taataattat taatagccac atgctccaaa
     8221 tgcaaatcta gttttaatct gttacaaata gaatttccat aacatacaaa accaccattc
     8281 caatatgcac cacaagaact tgtagaacct ctatatacag gatttccacc caataatctt
     8341 atagtctttg ctaatatctc catatgtaac atttctgtta tagatatatt tatccacatt
     8401 tcacttaact ctctatgcgt ttcatctaaa tcaaaattat ggtatagata ttgatttaca
     8461 ctcgtaaatt cacttgatac tccggcatag tcatccatca ataattctgc ataatactta
     8521 tttagaccta aaacttttat ctctggatat ggctcatcac ttgaatatcc atttcgcttg
     8581 tgcatgtcgt aatgtgcatc actcataata tctcctcctt taaatttaat acttgatatt
     8641 aattgtatgc atcacgactt tttcatataa cttagataat aaaatctatt atattatttt
     8701 ctattataat ttatgtgtca attcacccaa atttatttga tatgcaatat ttctttcctt
     8761 agtgctacat atgtaataaa aacatatata caatatatta ctacaaaatt aataagcgta
     8821 atggctacag catgtaagat aggaatctga atattaaaaa atactggagc atcaaaagaa
     8881 atattaaaca tgttgtatga aaaaactgat accaatattc caccaaatat tactgcagga
     8941 attaatggaa tcgaaaaata taaacaaaca tgtttcttta agatttttat tacttcacta
     9001 tcaatcatcc ccaaagttcg aagcattcca tagtgtttta tgataatgtt attttcgctc
     9061 aataattgaa tagagagaat gactgtacca ataatgcata taatgagtgc caaataaaaa
     9121 agtggtaata tcaagataat ggcttgacca gtagattgca tagcatactt tccacttata
     9181 taatccttat tatcatatcg taatttagaa acaaagcctt gattacctgc tactactact
     9241 ccagaattaa gattttctaa agtatcaaac tttttctgaa actcagtaaa ataagcacta
     9301 tccaaaggct cacttgtaat agcaacataa agactataca gaggtttcat attatcaatt
     9361 aaataatctg gaatgacaat aatatattct tgtccatttc catatcctcc atactgtgaa
     9421 aagttctcta tataaatacc tgcaatactt aagtccttat ctgatattgt cagtttatat
     9481 ttttctccaa tataattctt atatgcatca gcaagataag gcatacaatg gatgataaat
     9541 tgatttttgt ccatctttat tgggtttaaa cccaacatat ttcttagtat attgtaatca
     9601 ctatatttca taaatgtgtc aaactggttt tctgcaaaag tataatcctt atattgtaaa
     9661 tctcgcccta tatctttcaa gtatttagaa aggatattat tacgaacctt cataaaggat
     9721 gttttttctt ctgtgtaaag agtataagaa taatcctttt tcacaccttt cgtttctgat
     9781 aaatatcctt catatgaaga aaaatcatat gattcattcg ggtgaaaaat actcacatca
     9841 aattgttgta aatccactga tttattcatc acagaataaa atgagttagc tattccaata
     9901 caagcaattg atactgtaaa aagagttgcg ataataccca ttataatgct tattccattt
     9961 atctttgaag ccagtagacg atacaaaaat aagtgagatt tcttatattt ccaattcatc
    10021 cctttttcaa acatattctg tgcaatagat ccaaatccca taaataatac gattagacaa
    10081 aatacaatca acaataaacc aagaaataca tctgttaatc taccactagg tattgaaaag
    10141 aaaaaaatac ccatacctcc acacaaaaca gacacacaaa acataataga cttcgttttt
    10201 gtttggtgaa ttaatacatt ttcattatat cgatcataat taagcaaatt aattaatttc
    10261 attttaacaa gttttttatt actttttaat tgagcaatta aatatattat gcagaagtat
    10321 aaaaaagtca tcccagcagc ttttaatgaa aaatccggag ataaagaata ctttccatga
    10381 aacatattaa taacaataga ttccaaaacc ttagaaacaa atatccctag aatccatcca
    10441 acaataagag cagaagcacc taataaaaga ttctctataa aaagcatttt aacaatttgt
    10501 tttttatcta ctcctaatat catataagta cttatttctc tgcttctttt tttcaatata
    10561 aaattcatca tataactaac aaaccagcct aaaataaaag taataatgaa cgaaaatatg
    10621 ataatcatat aagaaaactc atcgcttcct ccattattaa gtatactaga aagttcttgc
    10681 attacgtcgg aaaatatcaa agcgttaaaa gcataaatca aagctacaga gcctattaag
    10741 gaacaccaat aaataaggta ctttcctgct tgtcgtcttg cattacgaaa agataatcct
    10801 ttcaacataa tcaatctcct cccatcaaag aaagtgcatc caaaatttcg tgatagaaag
    10861 taagtctgga tttttctcct tttctaagtt cgttaaaaat tttcccatcc cttagaaata
    10921 aaattctctc agtataacta gctgaaatag cgtcgtgtgt gacaagaaga atcgtagcct
    10981 ctaatatggt gtttatagtt tccaaaagtt ccattaaagt tttagatgaa ttagaatcca
    11041 atgcaccagt tggttcatct gccaaaataa gtttgggctc tgttacaact gcacgagcta
    11101 ctgcacaacg ttgcctctga ccaccagata cttcataagg aaacttcgaa agtatctctt
    11161 caattttcaa ttgtgtggca atattataca cactattagt aatttcaaca ggagaaactt
    11221 tttttattgt aagtggaagt gctatatttt cttctaatgt cagagtatct aataaattag
    11281 aatcctgaaa aacaaaacct aaattatcac gacgaaaaac agttaagttt tcttcggata
    11341 gttcagtaat atcttgattt tcaagataaa tttttccagc acttactgta tcaattgtag
    11401 caatacaatt taacaaagta gttttaccag agccagaagt tcccataata ctgacaaact
    11461 ctcctgtatt aatttgaaat gacacatcat tcaaggcctt tgtaagtatt ttctgattgc
    11521 cataatattt ctcaatgttc tctactcgta atagcatgtt aaagtcctcc ttagtcaaca
    11581 atttttccat ttttaattat tattgtctta tctgaaaaat tcaagatata tacatatatg
    11641 ctttctttag agtcttgaat ttgaatagta ggttcattag atgaaattag agattttttt
    11701 acttcaatat cgtattttat tgttaattca ttatcatctc catttttatt agtatcttct
    11761 acatttttat ttaaatcaat gttattatga ttaaagggac cagtaaactt tacttctgta
    11821 atagttccat tttttctaat aaaagaagca tattcagacc ttagtggtat gatacttcgt
    11881 gagaaaaaac ccatcgaatc caatcctgta attgaaataa acaaaacgag tacaacacat
    11941 aatatagtta atatcctttt tttcatatgt ttctccctcc tttctattga tattgtagcg
    12001 taaatagtaa tctgaatata tagaagttac ttaactttat cttacagttt tgaaagataa
    12061 agaaatagca gcgcattttt agttgcactg cttctgcata ttgatagctg tatttattgg
    12121 gaaattcaat gttattgctg tgtattcacc aaggctggag gacacatcta tatcaattcc
    12181 cattttctta gacagttcat aacaaaggta aagaccaatc cctgtagaat tgtttgtagt
    12241 ccttccatta ctgccagtaa aacctaattc aaaaattcga taaatttcac tttctttgat
    12301 gccaatacca ttatctttta tagtaaactt aacactcttg tcttctttta ttgcaaaaaa
    12361 tgaaatttca gctgaatcac ttttgtactg aacactattg ataataattt gatttaaaat
    12421 aaaaactatc cactttttat cagtatatac actatactcc aaatcctgta tattaatatg
    12481 aatattattt tgtataagaa gcaatttatt ctttgctaat acttcttcta caattgactt
    12541 taatgttatt tcagaaatta aataatcttt ttcaacatgg ccaaggcgag catagtacaa
    12601 tgcacgctca atatgacgtt ctatttcttc catttgaaaa cgaatttttc gtgtatgttc
    12661 atttttataa ttttcacata taagttctga tgttgtaaca ggatgtttta tctcatgtac
    12721 ccattgttca atgaattgtc tatattcgta catttcacga tgtatttgag aaacatgatc
    12781 tgtcatagat ttcaatgata atctaataat attgtagtat tcacgttcta tgttagaagt
    12841 aggtttttca atcatttcaa taaacagata ttttttatcc aattcgtcta caatgcgacg
    12901 taacttaata atttcttttt tctgtttttg ataacaaaaa catatccaac aaaataaaat
    12961 tgcgacccaa gcggctataa gaagagtcaa ctctccttga ccaatcccta taagatgaag
    13021 atatagcact aatgaaattg cacaacaaat atttaagagt ataactaata ctttatcttg
    13081 aagaaaatta caaaacctca tatcctatat cccatccccc gttttgtaat gattaagtca
    13141 gtaataccta taccccttaa cttttctcta agccttgtaa tgtttacact taaagcatta
    13201 tcatcaatat gaatatgatt gtcccataaa tattctacca aatctatacg aggcacaata
    13261 ctccctacat tctgaaacaa ataatacatg atttttagtt cagttcttga aagttctata
    13321 acagcatttt ggtattctac tgtagctgaa ataaggttga gtataatctc tctatattta
    13381 attttctgtt tatctgatga atctactctt ttaagtaggt tctttaccct tgctaaaagc
    13441 aaaggtaagt tatatggttt tgtaatatag tcatctcccc ccatagtcaa accatttagt
    13501 tcatcaattg cactatctct tgctgtaata aaaataatag gtatagtaga acaattccta
    13561 atctgtctac ataaagaaaa accatctata ttaggcaaat tgatatccaa gagaattaag
    13621 tcaggtgttg attcttggat tacttgaagt acatcacaat tatttgtatc caatgtttgt
    13681 gtactatagc cattattttc aaagaattgc attaattcct tacagatgaa cacatcatct
    13741 tcaattatca aaatattggt taaaataata aatacctcat ttctttagtt tatattgctt
    13801 taattttgta agtaatatat ttttccatat attacttaca aaattacttt ttaattattc
    13861 atcttttcca gctactgata gctctttaat aacaactgaa ggtgaaccta tactactcat
    13921 tggaaattct aaatcttccc ctataacttc aatatctttc aacaggtcaa aatagtttcc
    13981 tgctactgta atttgctcca ctggaaatac tttttttcct tctttgatat aaaagccttt
    14041 ggcagctaaa gaaaagtctc ctgttactga atttgcacct gaatgaagcc ctgcaaagct
    14101 agtaaccatc aatccttcat ctatatcctt tataatttca tctaaagatt tatctccttt
    14161 atctatataa aaattagttg gatatatgct tataggtgat gaatatgaag attttattcc
    14221 atttcctgta gtctttacac ctgccttatt agccgttttt aagttatgaa gtaaagttat
    14281 aagctttcca tttaaaacaa cttctttttt aaatgtagct actccttcat catcaaaagg
    14341 agtagatgcc ataccatttt ctaaaagtgg gtcatcaact attgtaacta taggagatgc
    14401 tatcatgtct ccttctctat ttttcaataa agataatcct ttttgagctg aatctgcatt
    14461 aaatatccca gaaaatgtgc taagcaaaga taccatagct tcattaagca atatagtttt
    14521 atatttacct gatggaatac tcttaccacc tactttagat aaagcattat caacaccaag
    14581 ttttgatatc tcacaaggtt ttatttcttc aatagaattt gctctattat atcctattcc
    14641 atcatatttt tgtccattgt cttctatgat aggaactaca aaccctataa gcatattagt
    14701 tttattgctt aaattaagac ctttagtatt agatatacca ttacttgata cagaataaga
    14761 tatagtacat ttagataaat ttaccacctt atctgaataa gctttagtct ctctttccat
    14821 ttctaaagct aaatcaatca acttatctgc ctctaaattt tctagctctt tagaataagt
    14881 ctttacatca ttatagtgct tatctccttc atatataaat tgtacatctt cattttcaat
    14941 agtgtttact ccatctttta cattttttat caacatatca acagctttat catctaaaat
    15001 ttctgtataa gaataaccta tctttccatt taccttacct ctaaaagata aaccaaaaga
    15061 tttatcaaga ttgtatttct caacttctcc ttcatatata tttatactta aattttctcc
    15121 tgtataataa tatatttcac actcttcaaa accttcactt agagcctttt taaataatat
    15181 atctttaaaa tttttaagtt ccacaattat ccctcctatc ttccacctac agtaatttct
    15241 tttaccctta tcataggttg gcctacatta gttggtatac ttcctgatga tgagccacac
    15301 atcccctgag cttgttttac gttattccca accatatcta tattcataag tatatcgcta
    15361 ccttttccaa ttaaactagc acctctcact ggctcttgta tttctccatt ttttattaag
    15421 taaccttctg aaactgcaaa attaaactct cctgttacag gatttacaga accaccaccc
    15481 atttttttag cataaagtcc atctggtatt gactttataa tgtcttctgg tctatcttct
    15541 ccagcagcaa tataagtgtt agtcatcctt gaagttggtg caaatttgta gttctgtcta
    15601 cgagaacttc ctgtaggatt cattcccatt cgtctaccat taagtttatc tatcatataa
    15661 gacttcaaaa ttccattttc aatcagtata ttcttttgag ttggatttcc ttcatcatct
    15721 atattcatag aaccccagta gtttggtatt gtaccatcat ctatagctgt aatctttgtg
    15781 gaagcaatct gttgacctaa tttatctgca aatacagagt tcccctttgc aactgatgta
    15841 gcttccaaag aatgtccaca agcttcatga aatattactc caccaaatcc attatctatt
    15901 gctaccatca tattacctgc aggacaattt ttagcatgca acatagtatg cgctactcta
    15961 gctgattctt ttgcataata ttctgggtct atttcttcaa atatttcaaa ccctttacaa
    16021 cctccaggac cttcaaatcc agtttgattt tcattaccct tagatgcaat tgaacttatc
    16081 cctagtctgg ttcttattct tctatcctca acatatatac cttcagtgtt tgcaattaat
    16141 attttttggt ctttatccaa atagctaaca cttacttgag atatatcatt actaaattct
    16201 tttgcactct tatatgcaac cttcataaca cttactttat ctctatttcc tattgaattt
    16261 ggataaattt ttatattatg tatgttattt attttaatac tgtcatttaa tattacactt
    16321 tttccatctt ccaatttacc taaagcaaca gcagctttgt atgctacatc caacagacta
    16381 tttaatgaat tgtcacttgt ataagcatat atactcttca atcctttaaa tattcttatt
    16441 cctataccat aatttcttcc acctattgca ttttctactt tattatcaat taaactaata
    16501 gaattattta tagtgtcttc ttcaaaaatc tcagcaaaat ctcctccagt aattagacac
    16561 ttttctaata ccttagaagc tatatttttt gaaagcataa atttacctcc tactttaaat
    16621 ttttaattaa cttttattca atctttttac ttgttaacaa acattatata ctatattact
    16681 taaaataagg taattatgaa tatattatac atatgtttta tactaaaatg ttatcacatt
    16741 ttatctatac atacccatat aactagataa ttaaaatgta taatattatt tgctatagaa
    16801 aataatacac tcatacataa taaattttag gagtgaagta tatgaatcaa gaatatgtaa
    16861 aaggaaatcc tatttttact aatattaata aagttccaag acaatttcca taccttactg
    16921 atgatattga tactgatgtt ataattgttg gtggtggcgt aactggatgt atttgtgctt
    16981 actatctagc taaaaacaat ataaagtctg tcattcttga aaaaggaagg attgcacatg
    17041 gaagcactag cgtgacaact tctctattac agtatgaatt agatgacaat ttgatagatt
    17101 taacagaggt tatgactcta aaagatgctc taaaagcata taacctttgt acttcagcac
    17161 ttgaagaatt agatacattt ataaagctat atggaaataa gtgtgattat gccaaaagag
    17221 atactctact ttatactgcc aacaaacttg aaattaaggc tatcaaagaa gaatataact
    17281 taagaaaaga aaatggattt gatgttgaat atattgatga atccacaaac ccttttagct
    17341 ttgatttaaa atcaggattg atagcaaaga atggaggacg agaattagac ccatataaat
    17401 atagtcacca tttgattgat gtaagtttaa aaaatggtct tcaagtatat gaaaatacag
    17461 aagtaaaaaa agtagattta tcaaatgata aggttactgc tgaagtatcg tatggataca
    17521 aagttcatgg taaaaaatta attgtagcta cagggtacaa tacaagttta tttacaaaaa
    17581 gaaattttgc aactaagtca aatactttta atatagcaac taaaccactt aaaaacatag
    17641 cttcttggaa aaacaacatt ttaattagag ataactgtga cccatataat tatctaagaa
    17701 ctactaaaga caatcgatta attataggtg gtgaagatgt aagttttgat gatatagaaa
    17761 atgaaacttt agccaatgaa aagtatgata ttttagaaca aagattgaaa agtatgttta
    17821 aggatataaa agacattgaa gttgaatata aatattgtgg ttgttttgct tcaactttag
    17881 ataatttagg ttttattggt ccagataata aaaataacaa tctatggtat tgcttaggtt
    17941 atggagcaaa tggtatactc tttgcaatat taggaggtat aatgttaagt gaattatatc
    18001 ttggcaaaca aaataaaaat atgaaattat ttaaagtaga tagatttgat aaatagatat
    18061 tgtatattaa aagactataa aaagatgaaa ctttataatt ctaaaagtta actttgatac
    18121 agtcttttat aatgttcctt ttaaaaatac tttatttata attaaatata aagtaaaatg
    18181 ttaaaatata actgataaaa tttagtcata agataatatt taagtttatt tgatgaaata
    18241 tggaggaatt attatgaaag aaatactcgt tattgctccc actaaaggaa cctatgaaaa
    18301 atcaattcac atagttaaaa aaaacaaata tacaaatatt gatgttgtat ttggcaatct
    18361 aaaagaaggt atacctcttg ctgaaaaaag tattaatcat ggtactagaa ttatcataag
    18421 cagaggtgga acttacaata tgttaaaagc tacttacaat atacctatag ttgaaattaa
    18481 agtagatgct tatgatatta taaaaagtta caaagaggta aaaaattcca acgaaccatt
    18541 tggaataatt ggatttaata atgtcatcta cggtttcgat attatagaag aaatattgaa
    18601 taagaaaata actatgattg aaattgaaaa agaagaagaa atttatgatg ctatagaaaa
    18661 gtacagaaag aaaggtataa acacatatat aggagatacc actgttgcac atattgttaa
    18721 gagacttaat tgtaagggaa tattaatcga atctagagaa gaaaacatac ttagagccat
    18781 tcaacaagca gaacaaatac tcgaagcaac caaggatgaa caaaaaagaa gattacagat
    18841 agaagctatg acagattttg tacatgatgg aataataaca gttgataaaa attttaaaat
    18901 aacattattt aataagagtg ctgaaaaaat atttggtata aaaaaagaaa atgcacttta
    18961 tcataatgtt gtagatgtta tcccaaacac agttcttcca caagtagttg agactatgga
    19021 acctcaaatt ggtgaaatac aaaatatagg gagtacaaaa attattacaa atagagttcc
    19081 aataattgtt gatggccaga ttcaaggggc tgtttcaaca tttcaagata gcaaggagat
    19141 aagttctttt gaacatacaa taagaaggag cttattaagc aaaggtctat ctgctaaata
    19201 cacttttgat aatataattc atgctagtga aaagacaaaa gattgtatag aacttgcaaa
    19261 aaaatatgct tgttatgata ctcctatgat tattactgga gaatctggag ttggtaaaga
    19321 attattttgt caaagtatac ataactatag taagcgtaaa aatgcacctt ttgttgctgt
    19381 aaattgtgct gccatacctc catctctaat agagagtgag ttttttggtt atgaggaagg
    19441 ttcatttaca ggtgctagaa aaaaaggtaa agcaggtgta ttcgaactag cacatgaagg
    19501 tacaatcttc ctagacgaaa taagtgaaat ccctcttgaa cttcaaggtc gattattgag
    19561 ggtattgcag gaaaaacaag ttatgagaat tggtggtgat aaagttattc ctatagatgt
    19621 aaaaattatc tgtgcttcaa ataaagactt aaaaaatatg atgcgcaatg gtttatttag
    19681 acaagacctt tattttagaa taaatatact aacattatat cttccttcat taaaagaaag
    19741 aaaagaagat attataaaat tatcagaatt ttttatacat aaatattcaa gcaaatacaa
    19801 taaaacgcct ttaattatca ctccaaatat taaaaaggca cttcttgaac gagaatatga
    19861 aggtaatata agggaattgg aaggtatgat ggaaagggct gttattgtgg aatcatttga
    19921 ctcgatttta ttagaaaagg ttagcaatga atatacaaat aatatagttg ataaagataa
    19981 tagtgatttt gaatgcagta atttagattt aaaaacttta gaaaaaaaat atatctataa
    20041 aatttataac gaaaataata gaaatactac aaaaacctgt gaaattctaa agataactcg
    20101 ctccactcta tggcgtaagt taaaagaaat agaagatgat gtttaaaaat aatacatacc
    20161 tatatatatc aaaacgtttt atgtttaatt ttaatacata aggcgtttat ttgttttcaa
    20221 taaaaaatac aattatatga taaaaagaat ctttgtaagt gaaattctca tcaatcatac
    20281 ctttaattat aagtaatcta cagcatttca atcgattatt aaaaataatc atgataacta
    20341 aaaatacaaa ctctatatat tggcatagta attgctttta taatacctat ataaaaatga
    20401 aagggagtga tactagatgc acatcttaaa atcagtgaaa aagataccag gaggacttat
    20461 ggtctgtccc ttgttattgg gagcactatt taatacagta tgtccacaag ctcttgaaat
    20521 tggaggattc acaacatcct tatttaaaac tggtgcaatg agtatacttg ctctattttg
    20581 tctatgcaat ggagctcaaa tcaatatcag gcaagctggt aagccattga ctaaaggtat
    20641 agtacttaca gcaacaaaat ttataattgg tgcagtatta ggtattattg ttagtaaatt
    20701 tattggacct aaaggtataa ttggtattac acctcttgcc atagttgcta ccatgactaa
    20761 ttcaaatgga ggtctatttg ctgctcttgc aggtgaatat ggtgactcca cagatgttgg
    20821 agcaatatct atcttatctc taaatgatgg tccttttttt acaatgcttg cttttggagt
    20881 aactggactt gcaaatgtac ctatagtagc actatttgca gcacttatac caatactact
    20941 tggatttata cttggtaacc ttgatgaaga tatacgagaa tttcttgcac ctggtacaac
    21001 attattaatt cctttcttcg cttttccttt aggagcagca ttaaacttta atcaaattat
    21061 aactgctgga ctacctggag ttgtacttgg tttaggagtt actataataa caggtatggg
    21121 tggctacttt gtaatgaaac ttatacgtgc agaacatcca gcagtaggtg cagctacagg
    21181 tacaacagcc ggaaatgcag ctggtactcc tgaagcttta gcagctatag accctactct
    21241 agctacaata gcagctgttt caacagtaca ggttgctgct gctattattg ttacagcaat
    21301 atgttgtcca atgttagttt cctatctaga taaaagagaa aaaaagaaaa aatctatttt
    21361 taaaacaaat aaaaatattg aagtaaatta atacttaagg aggtaaaatt aatgaatgca
    21421 attattcatg caaatgaaaa agataatcta gttacatgtg tcagaccttt agttaaaggt
    21481 gaaaaagttt caattgatgg aaagtcttac atagtaaatg ataatatacc tgtctttcat
    21541 aagatggcta cagaagatgt aaaaaaaggt gatgttgtat acaaatacgg tgaaattata
    21601 ggaattgcaa cagttgacat aaaaactggt gactatgttc atgtacataa cattgaaagt
    21661 acacgtggtc gtggagataa aaaataggag gaagttaaaa tgaaattaat gggttattta
    21721 agagaaaatg gtcagtttgg tattcgtaat catgttttaa ttattccaac atctgtatgt
    21781 tcaagtgaga cagctacaag aatagcatca cttgtgcctg gagcaatagc aatccctcat
    21841 caacatggat gttgtcaaat tggttctgat atagagctta cagcaaagac attaatagga
    21901 tttggaaaaa atcctaatgt tgccgcagtt ttagtagttg gtcttggatg tgatggtatt
    21961 caagcaaaag aattagcatc tgaaatagct acaacaggaa aaaaggtaga ttacgttgtt
    22021 atacaagaat gtggtggaac attaaaaaca gtttctaagg gaactgaaat agtaagtaaa
    22081 atggctcgtg aagtatcaaa agaagtaaga gttgaatttg gtatgagtga aattacatta
    22141 gcacttgaat gtggtggttc tgacccaaca agtggaatag cttcaaaccc ttcaataggc
    22201 gttgcatcaa accttcttgt agatgaaggt ggaagtagca tactaagtga gactacagaa
    22261 gtaattggtg cagaacattt gttggctacc cgttttgaag atgaagaaat gaaagataag
    22321 tttcttaaat ttgtaagtga tgtagaaaaa agagcaattg ctatgggaga agatttacgt
    22381 agtggtcaac ctactccagg taataaagct ggtggacttt caacaattga agaaaaatca
    22441 cttgggtgta tgtataaggc aggcaataag ccattcaaag gtgctcttga atatgctgat
    22501 attgtacctc ctgataaaaa aggattatat tttatggata ctccaggtca agatatagat
    22561 tctatcactg gtatggtagc aggtggtgca caaattgtaa tcttttcaac tggtagaggt
    22621 actccaacag gaagccctat ttcaccagtt attaaaatca ctgggaatag tgatacatac
    22681 aacaaaatgc cagacaatat cgatataaat gcaggtagaa ttataacaga tggtgctaaa
    22741 atagcagata taggtcaaga aatatttaat gaaataatag aagtctgtaa tggtaaacat
    22801 accaaagctg aaagtcttgg tcatagagaa tttggcattt acagaatttc atctacattt
    22861 tagattgtaa caaaatatct tttataaata taatacttat gtagatacat aagctaaaag
    22921 ttctttctaa tcctataaaa attttctata ctgaaaagga actgtatcaa aattattttg
    22981 aaaacagttc cttttttaac ctcttttact caaaatatta taaaataaga tttaaacaat
    23041 aaacgtgtct tgattaatct ctaagaagac ataaataaag ctaaatgtaa agtgagataa
    23101 ataaaattta atatctttgt atgaaaagat atttggtata atatgtctga gagaattctt
    23161 atttgtaaac aaataaagga tggaggatga aacaaaagaa tgaactttga aaacacaaaa
    23221 aataaaaaaa taatagctac tttactattg acactccttt taatcgtgtc attaacatgt
    23281 gtcaatttta ttatgaatct aaataaagta acacataatg aaacagaacg atatcttgta
    23341 gaagtttcac aaagtatttc cactacaatt aatgtgaaat gtaaaagtat atttaggaca
    23401 ctatattcta tgggagatac ttattttgag attaatgata gtgaaaaaga agtcaataga
    23461 tacttatcaa aaaaattaaa attatttgaa tttgattggt taggtattat agattctaat
    23521 ggaaattttg taagtgccct taaagataca ggtaatgtta aaggacaaga cttttataga
    23581 aaagcccttc aaggtaaaga aacaattact cctgttaaat cacaatctgg tacatatgga
    23641 attttatatt ctgtaccact atacaatggc gataaaattg ttggagctgt tgctgcttgg
    23701 aatactattg atactatgag aaatttatta agtgtagaaa gctttcatgg tgaaggattt
    23761 tcccaaatta tcgaccaaga tggaaatttt attgtaagtt cagcaaacaa aaatgctcca
    23821 aaaaatgtgg taaatttttt tgacatgatt aaacaccaag gaaaacaggt tgataaaaat
    23881 aaacttgaag aaatgaaaat aaacttaaaa aagaacaaaa ctggttctat gtattataca
    23941 ttaaatgatg gtatttctaa ggcaatgcat tatcttccat tacaagaaga aaacttatac
    24001 ctcctttcag ttgttccaac aaaaacagca agtgcacagt ttgatttact tctaaaaaaa
    24061 gcaatcttca tcaatatttt aattgttata ctatttttat tgcttattat attgattatg
    24121 tttataaatc aaaagaacag ggcacaactt ataaaaatcg cctatgtaga ctctgtaact
    24181 aaaggttaca gtaaagcacg ttttgatatt gaagctaaaa aaattataca gtcatcttca
    24241 tcagaaagct attcattggt atctataaat attcagaaat tcaaacttat taatgattct
    24301 tttggtagtg aagcaggaga taaaacatta aagtacatac atgatgtcat tagtaattat
    24361 ctagaaccag gagaattgct ttgtcgagtt tctaatgacc agtttaatgt acttattaaa
    24421 actattcctc aaaaagaaat tcttaagtac atagaaaata tcaccaatga tattaatagt
    24481 ttcaataaaa atgaagaatg taaatatttt atagctctat ctataggtgt atataaaatt
    24541 gatgacccaa atctaccttt agtttcaatt caagaccgtg caaatgttgc acgaaaaaat
    24601 aataaaggaa acattggaaa tcatccactt tatacatgtg ttttttatag tgatttagag
    24661 cgtttaaaaa tgattaaaga aaaagaaatg gaaaatagaa tggaggaagc attaaataat
    24721 aacgaattta tagtatatct tcagcctaaa ataagcctca aagataatac tattgtaggt
    24781 gcagaagcat tagtaagatg gcaagaccca aataaaggcc ttataccacc agacgagttt
    24841 attcctttct ttgaaaaaaa tggttttatt acaaaactcg accactatgt ttttgaaaaa
    24901 gtatgtagta tgataagaaa atggatagat aatggagaaa ctccaatgcc tgtttcagtt
    24961 aatttttcac gtgctaattt aaataatata gattttttag aaaaatacag aatgcttcgt
    25021 aataaataca acattcctgc taagctctta gaaattgaat taactgaaac acttgtattt
    25081 gaaaatctcc aaaaattaat taatataata gaccaaatcc atgaagaagg ttttcaatgt
    25141 tcactagatg actttggaag tggatactct tcactcaata tgttaaaaga aataaaagtt
    25201 gacacatata aaacttgacc gtgctttttt tagctcacct aatgcagata atattgctga
    25261 aaatcatgta atagaatcta tagttaattt ggcaaaaaaa ttaaatatga atagtatatc
    25321 agaaggtgtt gaaacaatct tgcaaatgga ttatcttaaa aaaattaatt gtgatatgat
    25381 acaaggatat gttttttcaa aacctctgcc tcaagaaata tttgagaaaa tgacttttgg
    25441 aaaaaaacta gaagaaacca gtatggatag ttccgtttaa ataaaataaa aaacttgtct
    25501 ctataaaata attttaatca ttttataaag acaagtcttt ttattttcca atttaatttt
    25561 attttctaaa tttcttatac tcggcttgtt ttaaattccc tttttaagaa taataatacg
    25621 attatcatag caataaaatc tgcaactggc tgtgccaacc aaacaccatc taaattaaat
    25681 tgaattggaa gtacaatgat taatggtata aataaaataa attgtcttaa aagagttaaa
    25741 aacattgaat gtttaacttt acttatacac tgaaaataca caggtcctac aatagaaatc
    25801 cccattatag gcaatgtaat tagatttatg cttatacctc ttactgctat atccatcaaa
    25861 tctgaatctt tattaaatat acctacaaat acttctggga aaacttgtac tattaaaaat
    25921 ccaaatgaca atatgacaac tgacactatg atagataata ttaaagtttt ctttgcacgt
    25981 tcatattgtt ttgcaccata attgtatgat attatagttt gcataccttg attcaatcca
    26041 aacacaggca tcataaacat caataaaata gatgtaagtg ctgtcatagc cccaatagct
    26101 aaatctccac cataaacctt tagagaattg tttgtaaaca tgtgagttat acttatagct
    26161 acttccatag caaaaggtgt cattcctatt gcaaatacct tctttactat atctttattc
    26221 aattttaagt tacatttttt tagttttaaa gtggacttcc ctcttataaa ataatatgtt
    26281 acccaaaaga atactactat ttgacatagt actgttgcta tagctgctcc ttttatacct
    26341 aaattaaaca caaatataaa tatagggtcc aatatcaggt ttaatacaca tcctacaatc
    26401 attgtccttg ctgctaattt tggattacct tctgctctaa ttgcattatt aagagaaaat
    26461 gcaactaaat taaatatact tccaatcaat atgacactca tatagtcttt tgcataaaat
    26521 attgaatctt tacttgcacc caaaataaaa agtatatctt ctaagaaaac taatcctaat
    26581 atagttataa ttaaagagat aattatagat aatgtcaaag cattacctaa tatcttctct
    26641 gcttcttctc tatttctttc ccctaatttt atagacaatc ttgtgctagc acctactgat
    26701 accaacattc caaaagctat tattagagta aatactggca tagttacacc aagacctgca
    26761 atagctatag agccaatacc ttccatagaa ccaataaaag ctctatctac tacattataa
    26821 agtgaagtta ccatcattgc tattattgcc ggtatcgaat acctaaataa taaaatccat
    26881 attttttcat gtcttaatgc ttcttgattc tccatgttgt caccatttac accatgtcat
    26941 aagttataca aattggttta tttgttcttg ggtctgttgc tatttctgca tcaatattaa
    27001 ataattcttt tagattatcc ttcgtcataa catcatatgc atcaccttca caaactattt
    27061 tacctttctt aatacctatc atgtggtcag caaatcttgc tgcattatta agttcatgta
    27121 ttaccatgac aatagttctt ccttgttttt tatttagttc atctaataat tttagtatct
    27181 ctaattgatg tgccaaatct aaatatgttg taggctcatc cagtaataat atatcagttt
    27241 cttgtgccaa agccatagct atccatgccc tttgtctttg tcctcctgat aaatcttcca
    27301 tatttctatc cttaaattct gcaattccag taacttctaa actccaattt acaatctctc
    27361 tgtcttcttt actagaatta ccaaatccac ttttatgagg aaatcttcca taagaaatta
    27421 attctgaaac ggtaagacca ctaggagctt gagggctttg tggaagtaca gccatatcct
    27481 ttgctatttc tttagacttc tttttgtaga tgtcctctcc atttatatat attttcccac
    27541 tattagggct aataattcta cctatagttt taagtattgt cgattttcca caaccattag
    27601 cccctatgat ggtagttatt tttccttttg gtatatttaa atttaaattt tttataatat
    27661 ctgtgtttcc atatgataca tttagattct tagtatttat acagttcata acaacaccct
    27721 ttcctaatca tttaccaaca ttaaataaat aaagtacggc actcctatta tagaaactac
    27781 tatacctact ggtatttcta taggtgctag aagatttctt gatatagtat ctgctattaa
    27841 aagtaataat gttcctatca atgctgatac tggtattaat cttttatgct ttggtcctat
    27901 tatttttcta cctatatgtg gagctattaa acctaaaaat gaaatactac ctgctactgc
    27961 tgtcgataca ccagataaaa ttactgcata tattataagt cttcttcttt gtttttctac
    28021 atctacacca agacttatag atacttcatc acccaaattt aaaacatcta tatatttaga
    28081 tttataaata gttataccca taaaaatcac tataaatgga agcacagcta ttacatattt
    28141 ccaactggtt ccccaaatgc ttcctgatgt ccatgccata accctattaa attcttgtgt
    28201 tgtaaatttc aattggaata tcgtcaaaat tgaagtaaaa gctatgttta cacctatacc
    28261 aataagtagt aatcttgacg aatttatacc acctttccat gcaagagaat atataagaaa
    28321 tgctccaaat aaagctccta taagtgcaac aataggcatt gtaaatacag tcaagttact
    28381 cataccttca tatacattac cattcataag gtatatataa actactacaa acaatgcagc
    28441 acctgaattt attcccaaaa ttccagaatc agctaaatca ttctttgtaa ctccttgaag
    28501 tattacacct gctgttgata atgcagaacc aaccaaaagt gctattacta ttcttggtaa
    28561 tcttaattta aatattgcaa tttcatgatt acgctgaccc tgccctaaaa aagtctttat
    28621 aacatccatt ggttctatag aaaatgagcc catatttaaa ctcaataaaa aagtgataaa
    28681 aataagcacc attaaaattg atataacaag taatgtttta gaatttattt ttataatatt
    28741 tttcatagta cctatttctc cccctttcta acaaggtata taaatattgg cactcctata
    28801 agtgctgtta tagaacctac tggtgtctca tatggaggat ttatcatcct acttaatata
    28861 tctgtatata caagcaatac agctcctaat actaaagagc tcggtattat atacttataa
    28921 tctgaaccta ctattccttt agctatttga ggaactataa gacctataaa aataatatta
    28981 ccagacactg acacagatgc tccagtcatt aatattacaa gtactataca tataaatcta
    29041 acaaagttag ttttttgtcc taatcctatt gccacttctt ctccaagact taatatagtt
    29101 attcttggag ccataataat agctaaaata aatccaatac caccagctac aaatagcaat
    29161 tttatacctt cccacttcac tcctacaagc cctcctgata tccagaaact taattgttga
    29221 gataggttaa aatacataga tattgctgat gccaaagaaa ttagcaatgt accaagagcg
    29281 actcctgcaa gagctaattt tacattatta actcttcctc ttgatttaga acttataaaa
    29341 tatataaata atccacttat acttgctcca ataaatgcaa acatcattac actaaaactt
    29401 ccatgtataa gttgaggaag ttttttttga agtaccagtg atatcgaaat tgcgaatgta
    29461 gctccttgtg taattcctat aacagatggt tcagctatag gatttctcgt tattccttgc
    29521 attattgccc cagaaactgc taaaaaccct ccaacaagtg cagctgctat tgctcttgga
    29581 agtctaacat cctttactat tctcatattt attccatctt ccatttttat caaactatct
    29641 atgacagttg atagattcat atcttttgcc ccaatagtta tggataatac aattccaaat
    29701 acaagtacaa ctatacttac taatatcaac aaataagtta tatttttttt actcagctta
    29761 gtcaattatc ctacctcctt ttccctttta atcgtcagta taatatcatc tattaagtat
    29821 tttaatttta agatttatta aaaataggtg tgaaaaatga atatcattat ctcacaccta
    29881 ctaagttaag aattactttg ttaattcatt tttaactgat tctaataata actcttttcc
    29941 aattggattg tatgattgac tgaaaaatgg agcagcatca agtattgtta cattaccatc
    30001 ttttactgct cttatttgag accagatagc actattttct aaatcttttt tatctgcttc
    30061 tgtagcaatt acaactatat ggtctgcatc tatatctgtt aaaccttcca tacttaccat
    30121 tggtagtgtt attccatcct gttttggcat attcttaggt ctagctaatt tcatatcatc
    30181 attgattaga gtacctattc caccatcact aaataccata aattgacctg aacttgccaa
    30241 cactggtaaa taagtttttt ctccattctt ttctataact tccttaccta actttgtagc
    30301 tttttcatca tacttctgta gccaactttt agcttcttct tctttgtcaa ataactttga
    30361 aacatcagtt aacttgcttc tccagtcatt tgcataatct ttcatcataa ctacaggagc
    30421 tatttctttt aattggtcat atattttttc ttgtctttga ctcattatta ttaaatcagg
    30481 atttacttct aatatagctt ccatatccat agtatccatc attgagtgcc caactatctt
    30541 tacaccttta agctcttctc ttatgtaact tggtaattta tcagtttcat atgagtctac
    30601 atttgctgtt gcaacaggct tataacctgc taataatagt tcttcactac ttcctgatat
    30661 atcaactatt tttttaggat ttgatggtat cttaacctct cctttaacag attgtacaac
    30721 tcttgtctct gatttatctt tatcctctgt attcttatct gaacatgctg taacaagtac
    30781 taacgtagtt ataatagata taaatattgc aagtgatttt atttttttca tgattacttc
    30841 tcctacttgt ttattatact tacatgatat tgattatcat aagcacatat ttattatagc
    30901 atttttctat ttttagtcaa tattattttg taaaaggata aaaattccta attaatctta
    30961 attatatata gcttagataa ggttttctta tattaaacag tatttttttt aagaattgga
    31021 cacaaacaca atgactttta ggttaaacaa tgataaaatg caatttatag ataatgaatt
    31081 tagtaaggag gattcaaaac atggtacatt tagtatattg tgataatgca ggaaaaaaag
    31141 gagaaaaggt cttagataaa atcttggctg gaacaaaaac aatggttatt agaggtgctg
    31201 ctggtagaaa aataccacat agtagagtat ttgaagatga gttactttat tttatggaaa
    31261 aaggaactgg aaaaatatct gcaaaagcca ctgttaaaaa agtagaaaac tatgttaaat
    31321 taacagaaga tgaaatttct aaaattttaa atgaaaatca accaaaatta aatttatctg
    31381 aaaagcaaaa agttcgttgg cataaaaaat gtttatgctt agttgaattt gaaaatgttg
    31441 aagaaataac acctcttgat tttgaccatc aaggaaatat ggatgattgg cttattgttg
    31501 aaaaaattga agatgtagtt gttggtacta gtatacctta caattatgag aagtctaaat
    31561 tttaaagtat caataacata atactatatt gctaaactct acttaaataa aaaaagagtg
    31621 catttaataa ttataaaaat tatttttata cactcttttt taataataaa tttaaattac
    31681 ttactttcta atctttcata tacatctata aattcttctg ctaattgttg aaaattcata
    31741 gctgtcatta aatggtcttg tgcatgaata agtataacac ttatttcagt tttattacca
    31801 ttagcttcat tttgtatcaa ttctgtttgg aatttatgag ctttatgaat tgattctttc
    31861 gacttttcaa tacattctct agctttatct atattcccgc tctttgcttc ttttattgct
    31921 tcaaatgcta gccctttcgc atcacctgca aatccaatta ttccaaaaga tatctctatc
    31981 atcttctcat ccatttattc gacctcctaa atatctctat agcttgatat gactaatcca
    32041 ttcttttcta aaaattcttt gactttttta gatgtaagta tcttgtgctc tttcgttcta
    32101 tcaatagcat aagatgtaga atttaaaata taatcatcta aaaaagctgg gtgagacata
    32161 atatccacta catcatactt cattatttcg tctatatttt ttataaaata ttcttcactt
    32221 acattttcct tataaaaact atctattaaa ggaactactt tagaatactc taaattatat
    32281 tcaaatccac ctcttattgg aagattatac ttggttacaa tcttctctac tactggcttt
    32341 aaacttacta aagtatggat atgatggtga ctatctaaat gagatatatc tattccaagc
    32401 ccttttaccc tatctatttg agcacaaagt tctctataaa tctcatcaca atccattttt
    32461 tcaataattt cgtctgtaat cctcctaata aaaaaaccgt ctttatcaac aatagttttt
    32521 aagttagata ataatggtct accacaactt aaagtcatat gaactccaca atttaaagtt
    32581 ttattttctt taagtaagcc tactccatgt tctaccccag gcatatttgc catcatagaa
    32641 gtagacctaa ctataccatt attatgagct gaaattattc catagttcac agcttcacaa
    32701 tatccaaaat catctgcatt tattataatt ttagtcatac cataacctac tttctataga
    32761 caactatcta ttaaatctag gaagataatc tttatgagct tccaacattt cgtctaatat
    32821 agtttttgct aagtcatcag atggaatcaa tgggtttata caaagtgcca aaagagcaga
    32881 ttcataatcg ccatatacag ctgctttagc taccaatact tcaaatgatt tgatttggct
    32941 tactaatcca tgcacagaat ctggtaaata tcctatactt attggttttg gaccattttt
    33001 ggttattaca ctactcactt ctaccgcttg ttcatcttta aaatctctta tagaaccatt
    33061 gtttaatgta ttaactactt ggatatcttt tttgtcattg tatatagaac ttattaaatt
    33121 acaagcagca tcactatagt aagcccctcc tcttttttca agttgaggtg gttttacatc
    33181 taatttttca tccttatata attcaaataa ttgttcttcc aattctttaa ctacttgacc
    33241 tctagctttt ccctctttaa actctctcaa ctcatcctca agcatttctt tagttttata
    33301 gtagtatcta tgatatggac aaggtattgc acccaaagat tttacaaatt cagcattaaa
    33361 atcaattgcc tttatatttt gcatacttat atctgctttg acaaacttat caattgcttc
    33421 ttttgtaaca tcttctccat ctaaagatac atttagacca taaaccatat gattaagacc
    33481 tgcaaaatcc atacttattc tgtcactttc tacattaaat agtttggcaa catcattttt
    33541 taaatgaatt ggcacattgc atagacctat atatcttcta aaattagtat atttgaatac
    33601 agcctctgtt attattcctg ttggatttgt aaaattcacc atccatgcat ttggacataa
    33661 ttcttccaca tcttttatta tatcaaatat aactggtatt gtgcgaaggg ctttaaataa
    33721 acctccagca ccattagttt cctgccccat aactccatga gataaaggaa tagtttcatc
    33781 ttttattctc gcatctaaaa gacctactct aagttgtgtt gtcacaaagt ctgcatctct
    33841 tagagcttct cttctatcta aagtaagatt tattttcata tctactccag ctttttttac
    33901 catcctctta gctagatttc caactatctc taatttttct tttccagctt ctatatctac
    33961 aagccataat tcctttacag gtaactcttc atatctattt ataaaaccct ctacaagctc
    34021 aggagtataa cttgaacctc ctccaattgt aactattttt agtccctttt tcatctcatt
    34081 ataccatcct tacctataat ttaatatact ctacaaaacc ctatacattt atttaccgaa
    34141 ataaaactac ctagttgtta attttcaatt tattagcttc cattttttca gacgctatga
    34201 caaatggaat atatattaat attgataata ttaaattaaa tagtgctaaa gctccaccag
    34261 tccagtcacc tgtagccatc gctccaccta atataggagg tgttacccaa ggtattttag
    34321 gtaaaattac tggtgctact aatccaaaat ctattgctaa gtatgataca gcactacata
    34381 tcactggtgc aagtacaaat ggaatcataa atataggatt taaaactatt ggaagtccga
    34441 atatcaatgg ttctcctata ttaaatattg caggtggccc acctaatact atcatttctt
    34501 tacgtcttct cccagctatt aataaagcta ctattaatcc caataccatt ccagaaccac
    34561 ctaaatatac aaatgcatca aagaatgcac ctgcaagtac atggtatcct tccgttgctc
    34621 ctgcttgagc caaggcagca ttttctccac ctaatttcat aagtatagtt tctgtaaaag
    34681 gaagtgctat atttgcacca tgaagtccaa cagtccaaag tatatgaatt ataaatgcaa
    34741 taagcattgt agaacctaga ctatcagcta catttgatag cggtgctcct aaataagtat
    34801 ttaaaatatc tgttaagaaa cttccattag aaagcatttt tataaatatt cctattacag
    34861 taaatatcat tatagttaac attcctggta ttaactttgc aaatgatctt gaaactgctg
    34921 gtggaactcc atctggcatt tttatagtta atttctttac cttagataat cttacaaata
    34981 tctcagttga taataatcct attaatatag aagagaataa agctgctgta cctaagtagg
    35041 tttgttgtat cattccccat ccttcaacta ctgtagttcc tccctcagga actattttac
    35101 caatttgtgg agccattata aaaaagatag ataaagctat aagcccagat tgtaacccat
    35161 cactctcata agattttgct agaaaataag caacacctat tactaagaat actgccattg
    35221 tacctattgc tccccaccaa aggtcgcctc caaatgtggt ccaattttct ccaaatatac
    35281 ttgccatcaa atttttgtat gcttccaatg gtaagttatt tatcaatgtt cccaaggcac
    35341 ctaccatagt tataggtatc attactataa atgcatctct tactgctact aagtgccttt
    35401 gagctcctat tttagcagct acaggcacta tatatttatc cataaatgac atgaattttt
    35461 ccattttaca tcctccagta caactttatt ttgaactatt taactctaca gctctatcta
    35521 acacagcttt tccattacat cttccataat ccatcatatt tataacttct actggaatat
    35581 tatatggttt tgccatttca gttgcttttc ctaatacata tctaacttga ggccctaaaa
    35641 gtaagacatc acaattttct atttcatttt ttaagttcac ttctgatata gcccatattt
    35701 ttgcttctat accgttttcc tttgctgcat cctccatttt tgtaactaat aaactagttg
    35761 acattcctgc tgaacaagct aacattattt ttatcatttt ttcccctcca aattagattc
    35821 attaaatatt ttttaagtca taaatttttt aaactataca tttaaaatat gcttttctat
    35881 taattttttc ttaatgtata tatcttattt ttaaatgtat atacatttaa ttcttgatat
    35941 tattatatac caaatgtata tacatttcaa gaaaactttt tcattttatt taaattttat
    36001 tcgacaacag cattcaaagt taatgtatat ccatagtgat atgtgtgtga gtactcaaaa
    36061 atctcactat tttgtaactt acaaatttgg cttatatata aaagtggctc tggttcttct
    36121 aattttagat tatccataca ttcttcattc gatattacag cttgtacttc tctctctgac
    36181 atggctatct tgtatccaca cacttcttca acaaaattat aaagagaatt ttcacaatga
    36241 gtcttattta aatttggaaa taacttaact ggcatataag taatcatata tgaaactctt
    36301 gcatcattgg ctaatcttac tctttttatt tcccaaacat tttcttctaa acttatattc
    36361 aacttacttg ataaattttc atctgcctga actatttcaa gagataccaa tatattagat
    36421 actttatagc ctttactttc catctcttta gtaaatccac ttacagacga tatattatca
    36481 tatatattat ttgcaataac tatactacct acacctcttt gtctagttat atagccttct
    36541 ttaactagat tattaagtgc agaccttaca gtcattctag ttacattaaa ttcttcactc
    36601 agctgttttt cagaagggat taaatcccct tctttcaaac tgtctgaatt tattaactct
    36661 ctaacatgat tttctataac tttataaatt ggctctttca tttaattcac tccttattaa
    36721 ttaaatcttt tatataataa gataatttta tatacaaata aagaaaatgt ctatacattt
    36781 aagataattt aaattttatt cagacatctt gtacaacttt ccgagtttta ataaagatat
    36841 aaatagactt atcattaata taccttttaa tatacttgat aaggttatac tccaccatac
    36901 accatttaga cccaacattt ctggtctaga tataaagtaa gccaatggta ctcttgcacc
    36961 agtcagtata gtaattataa tagaaggtat ataggttctg cctaatcctt tgaaagctcc
    37021 tgttgttatt atttctaaac acataaaaaa ttgagaatag cctaatattt taagataaat
    37081 tgctcctttt tctattgcct cactttcatc tataaaaata gaaaatatac tctctcctgc
    37141 aaatactaat accaaagtag ttattatccc aagtacagaa gatacagtta tagctatttt
    37201 gcaaccttta tttattctag aatacttctt ggcaccataa ttttgtccaa caaaactgcc
    37261 taatgcaact gatagccctt cagccgtcat ccaggaaatt gcttctattt gtgagcctac
    37321 tttttgtact gcaacagcca caggccccca tgatgctact attactccta atagcattga
    37381 gaatactgta aacataccac tttgtattgc tataggtaat cccaatttat acagaacttt
    37441 ataatattta gtctctattt ctttaaatag tcttaattta aaatattctt ctttactttt
    37501 aactattata tatataaaac atgtagtaac tactatctga gcaaatacag ttgcaatagc
    37561 agctcctgca acaccaagtt ttggagcaaa tccccatcca aaaattaata taggatcaag
    37621 tataatatta gttattaatc ctactgtatt aatacgaaaa ggagttttac tatttccaag
    37681 accgttgaaa attgcagtaa atactggatt aataaaataa aataccatac ctagagctac
    37741 tattatcaaa tactgtctag acatggttat aacttctaag tctcctaatc taaaaaaacc
    37801 tatcaattgt ttattaagta ctattaaaga aattgtatat attattgcta gtataatatt
    37861 tatttctatt gctgacttca tatatgattt agtagtgctt atattatttg caccaatact
    37921 ttgtgctact tttatctctc cacctacttt tgaaatcata ataaatgcca ttgcaagcca
    37981 tggaaaaaaa ccagctgttc caactgcagc tactgcttta cttccggctt taccaaccca
    38041 tatcatatct atcatgttat accccatctg tataaatgaa gtccccatta ttggaagtga
    38101 tagcttaagt aatttttctg taattcttcc ttctgttaaa ttaattctgt ctttcatttt
    38161 ccacctcttt tatattaaat ttatttgctt atatagctag attaatcatt ttatttgatt
    38221 gagttcacta tccttcaatt tatattatca tatctcaaga ttgcatctca accatactta
    38281 tgtttaagat aaagtatttt acctatctct gataaaatta atacttatga atataaatat
    38341 agtcatttca tcaatacata ttataaccct taacttaact tcaattaagt atacatactg
    38401 taatcaatta gaatatttta taatgtatct tccattaaga aataacaaat ataagtgatt
    38461 tatctataat tttatttata cataaaatag tatactgcaa taataaaaaa actatatttg
    38521 aatactttaa gcatgtttac taattgtaat ttccacacaa atttgataat atatgaatat
    38581 attatggtga aatttataga tatatattaa ttatggggag attgtgggag gttcttttca
    38641 caagacattt aagctatgaa aataaataaa aaatttaata gagttgatat gcaggtttca
    38701 atacttactg caattattgt aataatatct tcctcatgtg tatatttttt taattataca
    38761 ttaacttata agagcatgat atactcttta agtgagaggt caaaacatat ctatgaatat
    38821 gtagagagaa atattgataa aaataccttt actgaaatta attctaaaga agaccaatca
    38881 aaaaagtctt ataaaagttc taaaatccta ttagaatctg taaaaaatac tactggagtc
    38941 atgtatctat atactgctaa aaaaacaaaa gatggatcac tagtatatgt tgttgatggt
    39001 ttaaattcat ctagaagtga ttttagaaat gctggtgatt tgatagaaaa agagatttgg
    39061 ggagatctta acaaagcatt agataatcat atattgttac ctaaaaagat taaatcaaca
    39121 tcttggggat acatatttat atcgtatttt cctattcaca atgatgttgg taaggttgtt
    39181 ggagtaatag gaattgaatt tgaagcaaaa catcaataca atacatttaa atttataagt
    39241 atagttacac caataatagc attattaact tgtctaatat cagcattgat tgcagtgatt
    39301 ctatttaaaa gaatatctaa tcctacatat actgattttg ctaatacaga ttatttaaca
    39361 ggcctaaaaa atagaaatgc ttttgaaata gatatgaata acttaaacaa ttcaaatcta
    39421 aagaatctaa tatctatcat atcaatagat ttagacggtt taaaaaagat taatgataaa
    39481 tttggccatc agacaggaga cttatatatt aagcaagcaa gtaaaattat acaagatgta
    39541 ataaataaaa aatatatagt ttatcgtatt ggaggtgatg aatacataat tattctaaaa
    39601 aatttatctc acaaagaaat aaatagtatt ataaataata ttaatttaaa aatagttgaa
    39661 gaaaactcaa ctaatgaatt aaaattatca atgtctattg gatatgctat ttttgatgaa
    39721 aaagtagatg tttctttatt tgatacatat cgtcgtgcag attctcaaat gtatgataac
    39781 aaaaagaaaa ttaataaata atatcaataa taaatttaaa aagtctattc tttaaataat
    39841 aaatcataag aatagacttt ttaaatttgt gtttttataa gataaattat ttaattaaag
    39901 tttgttcttt tttctaaacg attctttata aattcatata ttactttcac taatatattt
    39961 gtgactaata ttaacacagc tagtgctgct gcctgtgcag tactcccctt atcatctagt
    40021 tgtacaattg atattgcagc tacttttgaa cttgcagtat atataaaaac tagtgctgat
    40081 attgtcacca tagagtttag aaaaaagtac ataaagttct ctaaaactgc tggcaaacaa
    40141 agtggtatag ttacgttctt aagaattgat gcgctgttta ttcccattga tttagctacc
    40201 atgtccaatt ctttatctac tttcttaata gcagtagtag ctgttataaa tgctactgaa
    40261 aagaaatgaa ctatattaca tataaccatt ataagtaatg ttccatataa tccattaaac
    40321 aaatttttaa tgtatattgt atccataaaa ttaaactcta atttattaaa aaataataca
    40381 taagctatac ctaaaactaa tcctggcaat gccataggca atgtacttaa aaaataccca
    40441 aatgatttta aatatggagc tttttctgtt ttttcattta aatatgcaaa taagaaaaca
    40501 aagctcgtcc ctagaatagc tgtcaatagt gacattttta ttgaatttat aaatggttta
    40561 attccatcaa tactgttttt catagaaaag tgttccagtg taagtcttaa atcatatggc
    40621 caaactgata ttaatgagga taagattaca gttaacatta cagctattat accaaatgca
    40681 ataattccta caacccctcc taaaatacta tctcttaact tattagtttt aaccttatat
    40741 ggaattgact ttgaagttat atatgagttt tgattttttt caattttttt agttactata
    40801 aacgatatta ttgcaggtat taaaagtaat attgatacag ttgctcccat tggcatattc
    40861 tgttgcccta taatttgctt atatacatct attgataata cattaaactc tcctcctatc
    40921 acctttggag ctccaaaatc tgtaaaacat aatgtaaatg atacgaatgc agaacttatt
    40981 aaagagtatt ttatacttgg gattgttatg cttataaatt ctcttatctt gcttatcccc
    41041 atagtattac tagcttcata tagtctataa tcggttgaac ttagagatat ataaagtatt
    41101 tgaaaggatt gaggaaatac atatataatt tgtgccaata ttattcccac tgctccatat
    41161 attggaacat gcactccaag taaattagtt accaaacctt gatttccaaa taaatatact
    41221 agacctattg catgcatcat tgttggagca aacataggaa acatagctaa tgatttaaaa
    41281 aaacctttaa atttaatctt tgttctagtt aatgcatatg catatactaa tgcaagtgtt
    41341 agtgatatta cagttgatat aactgataca aatactgtat tttttagaga atttaataga
    41401 gattcagaag ccaagtattc ttgaaagttt tgtaatccta caaagactcc atctttatct
    41461 aaaaaagcct gtacaaatag atatattata ggtgctaata aaaatattac taaagacata
    41521 actaatactg taagcataca tttttttata tctaaattca tttttgattt aagacttata
    41581 cttttagaac ttatagcatt attattcata cacttatgct cctttaactt ttggatactt
    41641 taaatagtat ttttcgtcta tacttatttt tacaaggtca ccttctacta aatttaaatt
    41701 aacccactct tttatagaaa catcacttat tatgaaattt tcatttaatt tatcttttat
    41761 ttcaactctc aataaattcc ctctaaactc tatatttgat attatacctt ggtagttttc
    41821 ttttgtcgat ttctctattt gaacatattc tggtcttaca gttaatatag aatctccatt
    41881 atcaaaacag tttgtatctc ctataaattg agctgtaaat aggttttgag gattttggta
    41941 tatttcttct ggagtcccta tttgcattat atctcctcca tttagaatag ctattttatc
    42001 tgccatagtt atagcttctt cttggtcatg tgttaccatt attgtcgtta tattaagttc
    42061 tttttgtaat cttttaatgt ccattctaag cttgtgtcta acctttgcat ctaatgcgga
    42121 cattggttca tctaaaagta aaaactttgg ttcaagagct aatgctcttg ctattgcaat
    42181 cctctgttgt tgcccacctg ataatgcttt aggatattta tgagcttcat tagtcaatcc
    42241 aactgtttca agtacttcct taactttatt atctattttt tcttttgaaa cttttctctc
    42301 ttttagtgga aaagctatat tgttataagc agtcatattc ggaaataatg catatgattg
    42361 aaatactata ccaaaacctc tctttgatgg ctctaaattt gtaatatctt tatcctggag
    42421 aattatagtt ccactattta catcttcaag acctgctatt attctaagta atgtagtttt
    42481 accacatcca ctaggtccga gtaaacatag aaattcccct tcttcaatat ccaaactaat
    42541 attatttaaa accctttttt ggtcataact tttaaacaca ttatttattt ttaagtaact
    42601 catatcttat ctctccttat atagttattt ttttatttta aattttatat cctacttttt
    42661 cttctaaaat tctttcatct aaattattaa tttaaacctt acttactttc agattttgaa
    42721 ccgtattttg attcccaagt tttaagtact gtacttctgt ttttggcagc agagtttaaa
    42781 tcaacttctt ttataagctg ttcaaatggt tgtttagaat aaccttttgg aactggatta
    42841 tatgtcttta tggaagtaac tgctacttca tgtgcatatc tatccatagc tgaatcactt
    42901 attgcccaat ctaaaaatag ctttgcttct ttttttatat tatctttttt aactaaggca
    42961 tttgcttcta agtcccaacc tgaaccttcc tttggaaata caacctctat aggttctcca
    43021 ctagataatt gttgtgtagc tctatatcca agtgtaatac ctattggata ctctccagag
    43081 ccagctaact tcgcaggttt tgaacctgag tgtgtatatg tagctatatt tttatgtaat
    43141 ttatccatgt aattgtaagc atctttttca cccatcattt gaataagccc tgatactgta
    43201 agataccccg ttccagatga tgctggattt ggcattgtta taagtccttt atattctggt
    43261 tttattaaat cttcataact ttttggaata tctataccta gtttcttaag ttccttataa
    43321 ttaactacta ttgctgtctc tgggacactc attcctaccc aagatacatc atcttcagta
    43381 tctttaaatt ttttatcaac tttatcataa tttttaggct tatatgcttc aagtgcacca
    43441 tcttttttta gctctatcat gttcattgct gataatcccc atataatatc tgcttgtgga
    43501 ttatcttttt ctgctaatag ttttgaagct attacaccat gagaatccat aactatctct
    43561 acatctatat tgggatactc ttctctaaaa ccttccatat aataagctat ctgctcttcc
    43621 tctagtgctg catatattgt aagtttatta tttccttttc cttgagtagt ttgtggtgtt
    43681 gttgatatgc aacttgtcat aaatgtaatc actacaatgc acagacaagt aaatataaat
    43741 tctttcatat cttccccctt tgtgtttttt ccttttttta tttgtttttt attgtttttg
    43801 cttatgttta tataatacac ctaatttgtt aatataaagt taagtgtaaa ttaacagttc
    43861 gttaattttt agaaattttt gttaaatata ttatttttag ttaaatacag tctgttttta
    43921 aacaatatat taatttttat agtatctaca ttttaattta aacataaaaa ataagtctaa
    43981 cctctataaa gtagaatgtt agacttattt ttataagtat ttaattatac aaccaaatca
    44041 ttttttttct tttcttcaag tgctttttta agcaccatat ccttaacctt cataagtcta
    44101 gtagtacttg ctctctcatt ttcttcaagc ttcatagcta tatacttaat agtctcaatg
    44161 taatttggta tcattacatt ttcaagagca ttaacacgtc tacgtgtctt ttctatctct
    44221 tgtgcaagaa gctgtgcact tttttcactt tctgcaagtc ttaaaagtgg ttgcatagca
    44281 tctgagaaag cttccatagc agaatctaat tcacctgatg taaaagcaag accataagga
    44341 taaatatcac tttgattgtt ttcagtttta aagtcaaaca caggaacatc aacactcatt
    44401 atatttctcg tagaaacatc tactgaaact gattgctttg gcatcataag tgctgaaccc
    44461 agatactcct gactcataac tgctcttgcg attataaagt ttttataagc agtatcaaga
    44521 gcattttctg cttcttcacg caatcgctta ttttctctta caatctcaag aaattgtttc
    44581 ataagttcat caagtttatc tttaagaagc ttatgacctc ttgtagcagt tttaaggagt
    44641 ttcttaagtc tagtcatttc catacgtgtt ggatttatat ttaatctagc cacagatatc
    44701 actccttagg catatatttt tcaaaatatt catcacggat acgtttaagt tcactttttg
    44761 gtaacatact aagaagcttc catccgattt ctaatgtctg ctcaatagtt ctatcagttc
    44821 taaatccttg tgaaacatat tctttctcaa attcatctgc aaacttagca tacattaaat
    44881 caatctcaga aagtgcagat tcaccaagta tagccataag ttcttttgca tccttacctc
    44941 ttgaataagc tgcaaaaagc tggttcattg tatctgcatg gtcttcacga gtcttacctt
    45001 taccaatacc tttatctttt agacgagaaa gtgatggtaa aacatctata ggaggctgta
    45061 tatctttttt ataaagttca cggctaagta taatttgccc ttctgtaatg tatcctgtaa
    45121 ggtcaggaat tggatgtgtc ttatcatcct caggcattgt aagtattgga atcatagtta
    45181 tagacccttc atgccctttc atttttcctg ctctttcata catagtagca aggtcagtat
    45241 aaagatatcc tggatatcca cgacgtcctg gaacttcttt tttagctgct gaaacttcac
    45301 gcaatgcctc tgcatagttt gtaatatccg ttattataac aagtacatgc atatcttgct
    45361 caaaagcaag gtattctgct gctgtaagtg ccattctagg tgtagataca cgctctacag
    45421 ctgggtcatt tgcaaggttt acaaacacta cagaacggtc gattgcacca gttgctttaa
    45481 aatcgcttat aaaaaattca gcatcttcaa aagttatacc aactgcagca aatacaacgg
    45541 cgaacttaga atcagttcca cgtacttttg cctgtcttgc aatctgaact gcaagattgg
    45601 catgtggcaa accagaccct gagaatatag gaagtttttg ccctctaacc aatgtattaa
    45661 gtccatctat agcagagact ccagtttgta taaattcggc cggataatca cgtgctgctg
    45721 gatttattgg agctccattt atatcaagtc ttttatctgg tataatttct ggacctccat
    45781 caataggtct acccattcca ctaaaaacac ggcctaatat atctggtgaa actccaaagt
    45841 caagactctt tcctaaaaaa cgaactttac tttcagcaag gtttatacct gttgagcttt
    45901 cgaaaagctg aacaagtgca gtatctccat ttacctctag aactttgcaa cggcgaattt
    45961 ctccatttga aagttcaatt tctccaagtt catcaaattt tacaccttct acgccgttta
    46021 ttaacataag aggaccggct acctcttgta ttgacttata ttcctttatc atagttcttc
    46081 agcccccctc tttttaataa ggtctgcaag ctcgttgtct atttcatctt caattgaatc
    46141 ataagctgca tctatatcgc cttcttctac atacttagca cgaccaatct tttcaagaac
    46201 tgaaagcttg ataatatcgt ttatagtaac attttgcttg atagcatctt gtgctgactt
    46261 atagaatttt aatataagtc ccatcattct atattgttta tgaagtgatg tataagtatc
    46321 aacttcatgg aaagagtttt gatgaaggaa gtcttctctt atagaacgag taacctctag
    46381 tatcaatcta tctccatctg aaagtgaatc aaccccaacc aattgaacta tttcttgaag
    46441 ttcagattca acttgtaaaa gtttcatagc ttgagctctt tgagctgaga aattatcatt
    46501 tacattttta tctgcccata catcaacttt ttcctgatag agtgaataac tagtcaacca
    46561 gttaattgct gggaaatgac gtttatatgc taaagatgca tcaagacccc agaatacttt
    46621 tactatacga agcgttgctt ggctgacagg ttctgatgta tcaccaccag gaggagaaac
    46681 tgcaccaatt gctgtaagtg ttccttctct ttcatccata cctagacaat ttacctttcc
    46741 tgctctctca tagaattgag caagacgtga acctaagtat gcaggataac cttcttcacc
    46801 aggcatctcc tctaaacgac cactcatctc tctaagagcc tcagcccatc ttgatgtaga
    46861 gtctgccata agtgcaacac tatatcccat atctctaaaa tattcagcta ttgtaatacc
    46921 agtgtatata gaagcttcac gtgcagcaac tggcatatct gatgtatttg ctataagcac
    46981 agttctttgc ataagtgact cgcctgttct tgggtctttt aattcaggaa attcaagaag
    47041 aacatctgtc atttcatttc cacgctcgcc acagcctata tatacaacta tatctgcatc
    47101 tgcccattta gcaagctgat gctgtgtaac agtttttcca cttccaaaag gtcccggtat
    47161 agctgctacc ccaccttttg tgattgggaa aaatgtatct ataactcttt gacctgtaac
    47221 aagaagtgaa tctggtgttt tcttttctct atatggtctt tcttttctaa ctggccattt
    47281 ttgcatcata gttacaggta tatcattacc tttttcatct gtaataacac aaactgtatc
    47341 ttcaacagta aattcaccag aatatatttc ttttatagta cccttaactc cataaggaac
    47401 cataatttta cattctacta ctgctgtttc ttgaacgcca ccaattatat ctcctgctac
    47461 aaccttatca ccaacagatt ttttaggttt aaattcccac ttagttgttc tatcaagtga
    47521 agatacttca acacctttag ttatattagt acctgaaata tcatacattt tttcaagtgg
    47581 acgttgaata ccatcataaa tagttgaaat aagacctggt ccaagttcaa cactcatagg
    47641 cattccaaga gaaactactt cttcccctgg tcctagaccg gaagtttcct cataaacctg
    47701 tatagaagct ttatctccat gcatttctat aatctcgcct ataagatgtt tatctgaaac
    47761 tcttacgacg tcaaacatat tcgcatctct catgccctcc gctacaacaa gcggtcctga
    47821 cacctttaca attgtgcctg ttttcatatt ctctcacctg caatcatata ctttatcaaa
    47881 atttaaatat tccctcctat attttattag cacatatctt tagcaccaat aaaatttgag
    47941 attgtctttt attcaacttt catctattct ttaaataata tatctgcacc tatagcacgt
    48001 ttactagatt ctcttacttc attcatacct aatcccatac ttcctccaat acctggaata
    48061 actataatgg caggaagttg gtaatctttg tattttgcta ttgattcttt tgcaagcaat
    48121 gatgcctgtt ctgtaatata aattatcgca tattcatctt caaccaactt atgtatactt
    48181 tttttaattt cgtcactatc ataggctggg aagatatcta ttccaagagc taaaaatccc
    48241 atgatgctat ctttatctcc aactactcct accttataca taagcatccc ttagcctttc
    48301 ttttatagta tctgcgtcta tattattaag cttactagtc aaaataattc taattgtttt
    48361 tatctctgat tctttagcat ataaatatgc aattagtggt tctaatgtaa gaggcttaaa
    48421 ttttgctcct ctaacatatt ccataagata gtcatcacaa agtttttcaa agacagtaaa
    48481 accactatcc atgccatttt tacagacatc agagtaagaa gtagatttaa aacatgatgc
    48541 tggagtatct gaggaaaaag cttctaagaa tttttcctta tcaagtgagc cacctgaaac
    48601 aaacacattc ataaacatat caaaatcttt cttcatattt ttgactctta taaaactctt
    48661 tagatttgtt aaatcgcaaa ctttcataac ataatctatt acaaatttgt tttggctttc
    48721 tttagcagtt tctttcatac tggtaaaggt tgctttatct agaacaatat caaccatctg
    48781 gccattttgt gtcttattat aaacatcaaa tgcctctaat atagctgatg acattatctt
    48841 aggtaaatca ctaaaactac gatttgctaa agattctcta agcttaataa gaggaattgt
    48901 acctccatca attaaatatt tctcaccatc tacaccagaa atttcttctt taatcagcac
    48961 ttttaaattg tgatagtcat ttttataaag aaatacatca acaaattttt cttcaggagc
    49021 tagactttta agaatttcat atgtctttga taattcttga gtcaaaatat tctcaaaatt
    49081 atgtatatta ttatttgatg tatcagcata acctgcctca gctataattc ttaaagagtc
    49141 ttcagggctt ttggcttctg ccatttgaat aaacatttgt ctgttaagaa gctgtttttc
    49201 aagcactctt attcgtgcaa cggcataggg ataagttttt tcttctgcca ttttttaacc
    49261 cctccttaat taaacaaaat ttctgctgca atttgttcaa tatattcatg ctctactgca
    49321 attatggctt caaatgaata attgtactca atgtcacctt ttttaactat gaaacctcca
    49381 gtaatatctc tagtttcatc agacacttta atgccttttt ttgatagcac atcttttaat
    49441 gtttttttat cttttttaga taatactatc tctgaatcat ctgtacaatt tgatttttca
    49501 atcatgttta gtataatctc ttcatattca ttatctttaa gatttaaaag tttgttttta
    49561 gcttctaaaa ttacctcttc taaaatctct tgtttttttg ataatatttg ctttttagcc
    49621 tccatataag ctcctgatat ttccttagaa gccgcttttt ctgcttctgc ctctgcaagc
    49681 tttgtatagg aagccatttc cttttcagct ttttcattag ctgaatttac ttttaaatct
    49741 gcttcacttt tagctttatc caatatttct tgtgcttctt gtttggcatc agctataatt
    49801 ctgtcaatca ttttttgttc attacccata ggagtgcttc ctccttttca ttttttttac
    49861 ttatgaatta taatttataa ttaaccaatt gcaattccat tgtaagctaa gaaagaaaca
    49921 agtagtccta aaagagcata tgtttcaact attgctgcaa atgttatagc tttaccaagt
    49981 tcttcaggtc tttttgcaac tattccaact cctgctgctg ctgctttacc ttgtgctata
    50041 cctgatacta aaccaactaa accaattgga agtcctgcca taagaagttg taaaccttga
    50101 gctgatgttg gtattgcatc tccaccaata atccctgctt ttgataaaat aaggaaagct
    50161 atgataaatc catacagacc ttgtgtacca ggaagaagtt gtaaaactag aagttgaccg
    50221 aatttactag ggtcttctgt tacaacacct gctgcggctt gacctgctat tgaagtacct
    50281 ttagcagaac ccatacctgc aaataatgct gctatagcag ctcctgatat tgccaaaaat
    50341 tgaccatttc ctaacgcgtt taaaaattcc atatttacat ctccttttta ttaatgcttg
    50401 tatattttgt tttatactta aacggaacaa aaggaactcc tccgccttca taaaacttat
    50461 tgaaaaattc tacatactgt aacctacttg tatgaacata tgcacctagt gcatttataa
    50521 gaaggtttat tgtgtgtcct acaacaccga ttacaactgc aagtatcgga ccagctatag
    50581 cccctaaaag gttgataact tgtgctataa ctcctgttgt taaacaaagc gccataattc
    50641 ttgtataaga taatatatca gcaaaataac ttgttatgcc atagagactt gaaaatcctt
    50701 taaaaagctt aacaggaata cctttaaagc ttcttccttg agtaagtaca agacctatag
    50761 cacctataat tgctaaatat tttcctactt ctgaaaatac acctatgtcc cctgcaacaa
    50821 aaggtattat aagtagacat acacctgtta tacacaaata ccaaaaacct atgtcaaaga
    50881 ttgcatctat tacctttcca cttcttatta atgtatatgc ttttattccc aaacctacat
    50941 atatatggat aataccgaac agtaacgaca ttcccataag tgtcataaca tcctctaaag
    51001 gattaattaa tgcttttata ggaattaaat cccccaaaat acccccgaat ataagacccc
    51061 atacagtagt tgaaactcca cagataccaa taagtttaat taaatttcct tcaccttttt
    51121 gtatctttaa tttatataca acaaatccac agactactgc aattattaac ccatatgcgg
    51181 cgtcacttag catcattcca aagaatacaa caaagaagaa agttaaaatt gtatttgggt
    51241 caatatcttt tgtacttgga tagctgtaca tgttagttac actttcaaaa ggtgttacta
    51301 atttattgtt ttcaagcaga attgggaagc cttcttcttt acttccttct tcagtctgaa
    51361 cacaacaatc aaacttttct gtaatttctt taattaattc atctgccctt ttgcttggta
    51421 accaaccatt aagacagaat gttgtttttg ttttgacaag tctctctata atcttttttt
    51481 ggtctctttc tatagtgtaa aaatcatata ggttttcaag catgtgtata ctatctgcat
    51541 gtcttttaat ttcctctgtt ttgtccttag aaacgttttc ctgcctaatt atcatttcat
    51601 tgtatttctt gatagcttgt aacggtgtac cttcttcatt tggtaatgaa accactgaaa
    51661 aattaaattc ttttaaaact tctaaaacat catcaaaagt ttcttttaaa gtaattaaat
    51721 aaacatagtt cattaacttg tcagaattga caatcccaat aacactttct ggtatttcat
    51781 cttctaattt tactcttaaa gcatcaatat ttgcgcttat gggcacagtt ccaagtatag
    51841 tttttgtata cttagtttcc atattttcaa gaggaatatc aaagttcacc catggttcta
    51901 acatactttt attgttgtta agtaagttta tgttagacct tattgtacct atctccttgt
    51961 gcaaattgtt taactccaga gcttcagcat aaacttgccc ttctttttca ctatcaattt
    52021 tgttaaactc tctttttggt gcaagtaggg attttttttg tttcactagt gaagaaacaa
    52081 tattaattgc ttggtttaca tgagacattt tctgctctag cattataact tcagattctt
    52141 cactatcttt tacaagataa ttttttaatt cctcctcctc agtaagaaag gcactatcat
    52201 caatctgaac aaacccacgg ttcataagta gctttaaaat ctggcttttt tgagcttcta
    52261 agccaacaat agatattttg ttcatattaa ctattgccat attatctcac tacctttcca
    52321 ataaccgctt caatagcctt actttttctt tcttcagcag ttttttcaat tttcagacat
    52381 tcacttttag cattagctaa gattttatca acctctgctc tactgtcggt gactgccttc
    52441 tcaataagag tttcttccat aagctttgct tgttttctta catcagcaaa tatgctttca
    52501 catttgttta cagcatcagc tctgatagac tctgcatcag taacagcttt tactttgata
    52561 ctgtctgctg tctgctctgc ctcaacaatg ctttttatta tttcaaaagc catccaaatc
    52621 acctctctta cctttttact atgtatataa ccaattagat ttttttattc taattagatt
    52681 actacctcat tgttaaagta taaacagcat ttttatataa taattaatat ttttcaacgt
    52741 ttatttctta ataaaacata ttttagaata gtacttgcct agatttacat ccttttttga
    52801 cttactttta gactatgaca tgagtatggt ttttaaatac cattattttc aataattata
    52861 atttaaagcg atatagtttg tcaatagcgt tattttgttt taaaattaac gctatgtcga
    52921 cattttaaat ttttatttta ttttttttat aattttaatt taatttatta catataaaag
    52981 ttttttatat tctttttcaa cctctaaccc ttgaagttta ttgttttttt tgaattttta
    53041 gaaatatctc gcactatttt atctaacttt tataaatcat caagctatat cgatttttgt
    53101 caccttattt tcaatctaaa ttttttatat atagttagaa ataaatcaat atttttaaag
    53161 attgataaaa cttacactta gttctaatag ttttatttta aggcaatgta ttaatcttaa
    53221 gtagataata tcattagtta ctctatatat tcgacaacat tataaaaaaa tcctcgtacc
    53281 aatcactcca atcttcaata ggatatgctg ctcctccatt tacacttttc agtaggtttg
    53341 atgtatattc ttctcccatg gaaaaaagtt cccctactgt tatatcataa cctctagatg
    53401 taacaacctt acaaaatgaa tccataacat cacgttgttc tcttgttttt ataagttcct
    53461 ttctaaattc atcatcattc ttagcttctt ctaaaatttt agttaatttt tcattcatta
    53521 tgtatttctc cttttaagta gtctttctgt aaacttcaat aagctttata taaaaaggaa
    53581 gccatcctaa ataaactaag atgacttcgc ttaactgtta gcaacacatt tactagtata
    53641 aaataattgg tgtacctggt tctatatttt catatatttt ctttgcctta gaaaaaggtg
    53701 tatttacaca tccatgtgaa cctgaagact tatatatacc tccaccaaaa gaatttctcc
    53761 aagtagcatc atgaatgcct atgttcccat taaatggaag ccaataactt acaggtgaac
    53821 tgtaaccttg accagttaat gaaacatttc tagccttata attaagagga tatatacctg
    53881 atggtgtagc aaatcctcta gctaaatcgc ctgttacaac atctgttgat acaagcattt
    53941 ctccattttt aaagaaccac atatgttggc cagaaagact aatctctaca tatgtatttc
    54001 caatatcatc tttattacga gatacagctt tttgtatgta aataggttct cttgtaacat
    54061 ttttaccagc ttttacaaca tcaactaatg cttcagcttc tttcttttta tctatcaacc
    54121 aaccataaat tccacctgaa attgtaatat ctttacctgt ggcagtttta aatggtcttg
    54181 agtcaccaaa agtactgtat tttttactta aacttcttgt atattctacc atagcatcat
    54241 tatcaaaagt tatgtcataa ttcttttctg agtctacttt taaccattta ttaatttcag
    54301 aaccatctaa agtttcctta cggtcactaa aatcatatgt aacgactaca ccaacatact
    54361 tatctaatgt cttaatagct tgtttcaact tttctgaatc ctttgtgttg tttggtgctt
    54421 cataaacttt actttcatcc agagaaatag aagtattccc agaattaatt gagttttgta
    54481 ctaacttata aaaatcatct tttttcactt tgtttccaag tacctctggt actataacat
    54541 aagcgttttt agtgctatca tacttaggat aagcactctt aggttctaca acatttttac
    54601 tatcaaaaca agataaagca ttaaaccttt tttctagtaa atctttatta tatgaaagag
    54661 ttatcatatt attatactct ttagatttaa atattccacc aatccatcca atagcgcttt
    54721 gactatcttt tatcttttga actttatcat ctggtgtaaa ttttaaatca atatcttttg
    54781 cttttatttg ttctttctta ttgtttcttt cctctaagtt taaagtatag gaattagtac
    54841 tagatgttaa caaatcattt acttgctcaa ctgtttttcc tgaagccttt acgccattaa
    54901 tagtcgttcc aaagtaaaaa tgatttctaa aaaagattgc cattcctata taagctacaa
    54961 acaaaacacc tacaactata gctatcttaa attttagatt tttttgtcca ctttcttgtt
    55021 ttacctcttt tccatcaatc ataattaaat ccttccttac attgtaatta cctatgtact
    55081 ttattggttt ttaaacaaaa ccatgtatgc cttattttat atacccaaat tactgtatca
    55141 ttgtttatat ttttaaatat atagttattt ccataaaacc attttatctt ttagacaaaa
    55201 tgactattat aaatcacccc tttcaaaatc ccttaaatac aatctctgct atattaatat
    55261 attaccatac ttttaataat cgtgggttat ctatctgttt ttgtaacact aaactactct
    55321 tttaaaaaag aaactaattt tatgttagtt tctttatctt aacttatttt tatattgaat
    55381 tttagcttaa cataacgttg ttatatagat tctatttaat tattttagtt tctatatctt
    55441 ttccatcagc atattcttta agtaactcct tagcatatcc aaaatattct tcaatatcag
    55501 attcttgact aaaagaaaat atacaaatta atatttcatt tgcatcttct gttatcattg
    55561 ctctttcaga atcactagtt ggactcccaa atttacccac tgaatcagac aatataggaa
    55621 gattttctat atttattaat tctttcccaa tacccttata ctgttctcct tcatctccta
    55681 cagtaaaaca tactggtgaa tgtaatttgc ttacattata tgtacctact ggatataatg
    55741 attttattga tattaaattg tttatttcta ctatattatt tattgtataa agacccttcc
    55801 cttgaataat tcttctaacc aatgcttctg aagatgttct atatctactt ggattttttc
    55861 caagctcttt ataagcattt cttgcatctt taattttatc tagtttcgta atgtcttcaa
    55921 cttttagact ttgatttata gtatcacaaa actcatttat ttcattaatt aatttttctg
    55981 agcttttatt tactgctact tttgcttgaa tgcaacccaa cttagcatta ggccatcttt
    56041 ccttaagttt gtcatcaata ctcacatcta tcatacttaa acaactccta tacactaaat
    56101 ttttattaaa gtttttatca tctatacatt atagtactat tcttttgtta ataacatgtt
    56161 tattagcaaa ataaatttat ttttaatata atttttataa aatacaatta ttttttgctt
    56221 tatattaaac aatacattga taaaataaaa aaacactaat tatttagcag tgatttatta
    56281 aaatcaaaca ttcataatag tgctttatat aaaaattaaa ttttgtttta aagtaaatct
    56341 aaaaaattct tagctggcat tggtcgtgca aaataaaatc cttgaactaa atcacatcca
    56401 aattgtttca ttaactcagc ttgttcttta ctctcaacac cttctataat tacaactaaa
    56461 tctagtgatt ttgctagaga cactattgat tttgctatat tgcgacttct ctcatcatgt
    56521 tcaataccat taagaaaaga cttatctagt ttcaatatat caattggcat atctcttaaa
    56581 caactaatag aagaatatcc tgtaccaaag tcatccatag aaatagaaaa tccatacttc
    56641 ctcaactcat ttaaaacatt ctttaaaata tttaactcat taatagccac agtttctgtt
    56701 atctctattt caattaaatc tgaacttaga ttatacattt gtattgtttt attgataaat
    56761 tttacaatat ctggttgata caactcaact ctagataaat ttattgaaac tggaactatc
    56821 tttttacctt gctttttcca ttcagataaa tcattgcaaa ccctttcaaa tacaaatctc
    56881 ccaattttta aaatcaactt acttttctct gctattggaa taaatactgc gggacttata
    56941 aaaccatgtt ctggatgatt ccatcttatt agtgcttcac tccctatcat ttctccagtt
    57001 acaagtgaaa acttaggttg ataatacact tcaaattgat tttttaccaa tgctattttt
    57061 atatcatcta aaatcattga ttcttcacta agcttattcc taacatcttc attataaata
    57121 gcataattta tatttttacc tttagcaaca gacctagcca tatttgcttt atttacagcc
    57181 ttttggatat ctacttcacc ttcttcaaca aaataaatac ccacacacag agaaatttca
    57241 aatactatac tcaaattact ttcaatatta tttcgtatac tatcaatttt tcttatacgt
    57301 ttattttttc catttttata atcacagaaa attgcaaaat aatcacttga aagtcgtgca
    57361 tatacttcac cttctgttaa ttcttctttt aaagcttttg ctatcgtaat taataaattg
    57421 tcaccaaatt catatccaaa agtatcatta atcatcttaa agttattaat atcaaaatat
    57481 actataatat aatttaaaga tggtttatct aaaagatact tactacactc tctataaaat
    57541 ttattttgat ttcctatatg ggtaacttta tcttcataag ccaaacttaa tatttctttt
    57601 tcattggatt tttttatata aattatataa aaaataattg caataaatat taatataata
    57661 aataacactg catatatagc tctgttgatt atttgttctg aattactaaa tacaacagtt
    57721 tctggaattg aaaaaatcaa attccaatct tttatataat tattttcact attcccatta
    57781 tccatagtag catatcctaa atatcttctt tcttccaaca tatttaatac agttgcacct
    57841 gttttatttt cttgcaattc tttttttaat ctatttattt ctcgaatatc attatcctgt
    57901 tttaacaatt tataaatatt ttttgatagg ctatcctctt gcggatgaag gataattgta
    57961 ccatcatttc ttacaacata agatatacta ttattttcat aaaaagatac attaataagt
    58021 ttcatcagag tattgatatt gtaagtacaa tataaaactc caactacttt atcattattt
    58081 aataatggta ctgaaatagt aatcttcttt atagacttat ttacttcatc aacatatgga
    58141 cttgaaacat acttcttacc tttaattgag tttttgaaaa attctctatc tttcactgaa
    58201 aacttatcac ctttggtatt ttcagcatta ccatttatat caactattgc catagtgtaa
    58261 aatttttgat tttccatttc atctaaaaga tttttcattt tttcctcatt aggtatatct
    58321 tgatttaaag cataatagtt agaaaaaata tttaaaacat ctaaatcttt ttcaatttgt
    58381 ctttgtgcta gttgagagcc ttgtactgca acttctttta tatgtatttt tgcctctgac
    58441 ataagaagat gttttgtatc ttctatatag atatacccca ttgtcaaaaa taatgatgtt
    58501 aaaaaaagaa ttgtaattaa tgtaaaactt ttatttctcc ctgtcattcg actcctcccc
    58561 taattagtca atacttcata tagataatat atccttttta taccgattca tcatttatat
    58621 tgtatataac tattcttaaa tgttacacat aaaatcaacc taataaccat aaaaacattc
    58681 ttttattatc ataataacac tatttagttg aattttaaat taaagaataa aaactatcaa
    58741 tttttctaaa caaaaaaact cttatacaat aaaactttgt tttatgtata agagttttta
    58801 ttttatctat tttatagtaa atttatcttc cataatatga atttaaatac atatctttta
    58861 tttcattcat aagtggatat cttggatttg ctcctgtaca ttggtcatca aatgcttgaa
    58921 tcaccatttc atctattgaa tccataaact taggttcttc aactccaaac tcttttatag
    58981 atttaggaat accaacttta agttttaaat cttcaatagc taccaataaa ctttggaatt
    59041 tctcttcatc agaatttccc tttaatccta aaaatgaagc tatttcagca tatcttttta
    59101 atgcttctgg atatttatat tgagagaatg ctcccatttt ctttggtgca tctgaagaat
    59161 taaatttcat aacttctgtt atcaaaagag catttgcaac accatgtggt acatggtgga
    59221 aagcacctaa tttatgagcc atagagtgac aaacacccaa aaatgcattt gcaaaagcca
    59281 tacctgccat tgtagaagca tttgccattt tttctctagc ttctgggtct tttgcaccat
    59341 tatcatatgc acgtggaaga tattcaaata tactcttaat ggcttgtaaa gctaatccat
    59401 ttgtataatc agttgcaagc atagatacat atgcttctaa tgcatgagtt aatgcatcta
    59461 taccagaagc agatgttaag ctttttggca tattcatcat catatcagaa tcaactatag
    59521 ccatatttgg catcagttca taatctgcta atggatattt tacccctgta tcttggtcag
    59581 ttataacagc aaatggtgtt acttctgaac cagtacctgc tgatgttggt atagctacaa
    59641 aattagcttt ttcacccatt ttagggaatg tatataccct ttttcttata tccataaatc
    59701 tcatagctag gtcttggaaa tctacatctg gatgctcata caatgtccac attatctttc
    59761 ctgcatccat tgcacttcct ccacctatag atattattac atctggatta aattctctca
    59821 tagctttagc gcctatctta gcacattcca aagttgggtc tggttctact tcaaagaatg
    59881 tagtgtgctt aatattcatt tcatctaata aatcagtaac tgctttagta tatccattat
    59941 tgtaaaggaa tgtatctgtt acaataaaag ctctcttctt attcattaca tctttaagtt
    60001 cttttaatgc tacccctaaa caaccttttt tgaaataaac ttttcctggt gatctaaacc
    60061 aaagcatatt ttctctcctc tcagctactg ttttaatatt aagtaaatgc ttaactccta
    60121 cattttcaga tactgaattt ccaccccaag acccacatcc taaagtaaga gaaggagcta
    60181 gtttaaaatt atataagtcc cctataccac cttgagaaga aggagtatta attaaaattc
    60241 tacaagtttt catggctgaa gtaaacttat caagtttttc tctttgattt acatcatcaa
    60301 tatataatga agatgtatgc ccaaatccac catcttctac taatttttct gcttttctaa
    60361 ctgcattatc aaaatcactg gccttgtaca ttgcaagtac tggtgaaagt ttctcatgag
    60421 caaaagcttc ttctatttca actgattcta cttcacctat taaaacctta gtatctactg
    60481 ggacttcaaa gcctgcaagt tttgctatag tacaagctgt ttgcccaact attttagaat
    60541 ttaatcctcc atttactaaa attatatttc ttattttttc tgtttcatct ttatctaata
    60601 aataagcacc acgctcttta aattcttttt tgacttcatt gtatatattt tctaatacta
    60661 taaccgattg ttcagaagca catatcatac cattgtcaaa agtcttagat acaagaattg
    60721 agtttacagc agtttttata tctgcactct catcaattat tgctggagta ttacctgcac
    60781 caactccaat agctggtttt ccagatgaat atgctgattt aaccattcca ggacctcctg
    60841 tagcaagggt caaatccact tctttcataa cagttgtagt taattctaaa gatggctcat
    60901 ctatccatcc tattatgcct tcaggagctc cagctaaaac agcagcctca agaacaactt
    60961 ttgcagcctc tatagtagaa ttttttgctc ttggatgcgg agagaaaatt attccatttc
    61021 tagtttttaa agctaaaagt gctttaaaga tagcagtaga agttggatta gttgtaggta
    61081 caactgctgc aataacacct attggctcag caattttagt cataccaaat gcttcgtctt
    61141 tctctataac accacaagtt ttagtttctt tatatgcatt atatatatac tctgaggcat
    61201 aatgattctt tataacctta tcttcagcaa ttcccatacc agtttccttt tgtgccatta
    61261 tagctagagg aactctttgc ttattagctg ccaaagatgc tgctaaaaaa attttatcta
    61321 cctgctcttg agtatatgta gcaaatattt tttgagcttc tctgattttt gctagttttt
    61381 taactaagct ttcaacgtta tttacagtat ctaaaccaac tgacataact tcattttctt
    61441 ttttattttc tattattact tgttctttat gagccatttt aacaacctcc tgagatagtt
    61501 ttttatttat tatgttaatt tattaacatt atagcattta ctttttctat tttcaagtta
    61561 tttttttaac attttatttt ttatctaata taacatcatt tctactaata atatctagct
    61621 ttacaaatac ttaatttaac tagatatcac tttatttttt acgcaaatca ctaataaaat
    61681 catctaaaaa cttaaggata acgttatttt tttaacgttt atatttttgt attttttgca
    61741 aataaaactt tataaaaaga atttttaata tgatattcta tcaagtttaa aaataaaaat
    61801 acttgaaaat gaaaataata acttgattaa caagttaaag taaatatagt ta
//
LOCUS       DAMJVX010000005        61845 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMJVX010000005 DAMJVX010000000
VERSION     DAMJVX010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 61845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61845
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.005"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            233..349
                     /locus_tag="PC407_000256"
     CDS             233..349
                     /locus_tag="PC407_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HDF2495738.1"
                     /translation="MDDDINMIAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            513..1580
                     /locus_tag="PC407_000257"
     CDS             513..1580
                     /locus_tag="PC407_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2495739.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PNSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            1772..2302
                     /gene="rnmV"
                     /locus_tag="PC407_000258"
     CDS             1772..2302
                     /gene="rnmV"
                     /locus_tag="PC407_000258"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDF2495740.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMQRIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            2473..3342
                     /gene="rsmA"
                     /locus_tag="PC407_000259"
     CDS             2473..3342
                     /gene="rsmA"
                     /locus_tag="PC407_000259"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDF2495741.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            3408..4679
                     /locus_tag="PC407_000260"
     CDS             3408..4679
                     /locus_tag="PC407_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495742.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDSDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEEYEELEEDEEIEDNEESDRDEDS
                     EEEYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSGDEEIEEFNNYKEIENYNRV
                     KVQEEYIDIEYDEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGV
                     SQGESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMP
                     YLGYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGST
                     GFLRYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            4830..5945
                     /locus_tag="PC407_000261"
     CDS             4830..5945
                     /locus_tag="PC407_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2495743.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            6131..7027
                     /locus_tag="PC407_000262"
     CDS             6131..7027
                     /locus_tag="PC407_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2495744.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASIVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            7097..7894
                     /locus_tag="PC407_000263"
     CDS             7097..7894
                     /locus_tag="PC407_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495745.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(7950..9302)
                     /locus_tag="PC407_000264"
     CDS             complement(7950..9302)
                     /locus_tag="PC407_000264"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDF2495746.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            9521..10399
                     /gene="purR"
                     /locus_tag="PC407_000265"
     CDS             9521..10399
                     /gene="purR"
                     /locus_tag="PC407_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDF2495747.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            10555..10833
                     /gene="spoVG"
                     /locus_tag="PC407_000266"
     CDS             10555..10833
                     /gene="spoVG"
                     /locus_tag="PC407_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDF2495748.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            11043..12422
                     /gene="glmU"
                     /locus_tag="PC407_000267"
     CDS             11043..12422
                     /gene="glmU"
                     /locus_tag="PC407_000267"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDF2495749.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEAEVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSNVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SVVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            12517..13467
                     /locus_tag="PC407_000268"
     CDS             12517..13467
                     /locus_tag="PC407_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDF2495750.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      14307..14392
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            14554..15075
                     /locus_tag="PC407_000269"
     CDS             14554..15075
                     /locus_tag="PC407_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2495751.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNDEGVTLTEAQSAKLLSDIGENKIYTSVTADNLGNPLTSNTKVDNKVLY
                     IVLIDNTVMDSTK"
     gene            15226..16902
                     /locus_tag="PC407_000270"
     CDS             15226..16902
                     /locus_tag="PC407_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2495752.1"
                     /translation="MAKKVRIGDKLVEKGYITEDQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            16914..18122
                     /locus_tag="PC407_000271"
     CDS             16914..18122
                     /locus_tag="PC407_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2495753.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            18137..19849
                     /locus_tag="PC407_000272"
     CDS             18137..19849
                     /locus_tag="PC407_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HDF2495754.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETHDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            19842..20750
                     /gene="pilO"
                     /locus_tag="PC407_000273"
     CDS             19842..20750
                     /gene="pilO"
                     /locus_tag="PC407_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HDF2495755.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            20777..21343
                     /locus_tag="PC407_000274"
     CDS             20777..21343
                     /locus_tag="PC407_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2495756.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            21380..21907
                     /locus_tag="PC407_000275"
     CDS             21380..21907
                     /locus_tag="PC407_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2495757.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            21928..23466
                     /locus_tag="PC407_000276"
     CDS             21928..23466
                     /locus_tag="PC407_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495758.1"
                     /translation="MRKWNKFKSERGAALVLVLIVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEKNIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            23566..24648
                     /locus_tag="PC407_000277"
     CDS             23566..24648
                     /locus_tag="PC407_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDF2495759.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESIFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            24629..25411
                     /locus_tag="PC407_000278"
     CDS             24629..25411
                     /locus_tag="PC407_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2495760.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGILFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILIFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            25513..26163
                     /locus_tag="PC407_000279"
     CDS             25513..26163
                     /locus_tag="PC407_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2495761.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     AQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            26177..26737
                     /locus_tag="PC407_000280"
     CDS             26177..26737
                     /locus_tag="PC407_000280"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDF2495762.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            26754..30140
                     /gene="mfd"
                     /locus_tag="PC407_000281"
     CDS             26754..30140
                     /gene="mfd"
                     /locus_tag="PC407_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDF2495763.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGAENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            30171..31166
                     /locus_tag="PC407_000282"
     CDS             30171..31166
                     /locus_tag="PC407_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2495764.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            31290..31829
                     /gene="spoVT"
                     /locus_tag="PC407_000283"
     CDS             31290..31829
                     /gene="spoVT"
                     /locus_tag="PC407_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HDF2495765.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            31949..33562
                     /locus_tag="PC407_000284"
     CDS             31949..33562
                     /locus_tag="PC407_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2495766.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            33669..35117
                     /gene="mazG"
                     /locus_tag="PC407_000285"
     CDS             33669..35117
                     /gene="mazG"
                     /locus_tag="PC407_000285"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDF2495767.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            35206..35484
                     /locus_tag="PC407_000286"
     CDS             35206..35484
                     /locus_tag="PC407_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDF2495768.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            35556..35798
                     /locus_tag="PC407_000287"
     CDS             35556..35798
                     /locus_tag="PC407_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDF2495769.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            35860..36114
                     /locus_tag="PC407_000288"
     CDS             35860..36114
                     /locus_tag="PC407_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HDF2495770.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            36115..36648
                     /locus_tag="PC407_000289"
     CDS             36115..36648
                     /locus_tag="PC407_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495771.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            36654..36938
                     /locus_tag="PC407_000290"
     CDS             36654..36938
                     /locus_tag="PC407_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDF2495772.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            37051..37971
                     /locus_tag="PC407_000291"
     CDS             37051..37971
                     /locus_tag="PC407_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HDF2495773.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            38061..40433
                     /gene="spoIIE"
                     /locus_tag="PC407_000292"
     CDS             38061..40433
                     /gene="spoIIE"
                     /locus_tag="PC407_000292"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HDF2495774.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNNEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            40784..42676
                     /gene="pepF"
                     /locus_tag="PC407_000293"
     CDS             40784..42676
                     /gene="pepF"
                     /locus_tag="PC407_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2495775.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAIAVMNNN
                     DTEDDAEKKQEVDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMSDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESVDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            42790..43755
                     /gene="galU"
                     /locus_tag="PC407_000294"
     CDS             42790..43755
                     /gene="galU"
                     /locus_tag="PC407_000294"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2495776.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            44255..45859
                     /gene="rho"
                     /locus_tag="PC407_000295"
     CDS             44255..45859
                     /gene="rho"
                     /locus_tag="PC407_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDF2495777.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQENRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            46012..46212
                     /gene="rpmE"
                     /locus_tag="PC407_000296"
     CDS             46012..46212
                     /gene="rpmE"
                     /locus_tag="PC407_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDF2495778.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            46357..47250
                     /locus_tag="PC407_000297"
     CDS             46357..47250
                     /locus_tag="PC407_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HDF2495779.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            47268..48116
                     /gene="prmC"
                     /locus_tag="PC407_000298"
     CDS             47268..48116
                     /gene="prmC"
                     /locus_tag="PC407_000298"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDF2495780.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            48160..49224
                     /gene="prfA"
                     /locus_tag="PC407_000299"
     CDS             48160..49224
                     /gene="prfA"
                     /locus_tag="PC407_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDF2495781.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            49353..50033
                     /locus_tag="PC407_000300"
     CDS             49353..50033
                     /locus_tag="PC407_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDF2495782.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            50062..51102
                     /locus_tag="PC407_000301"
     CDS             50062..51102
                     /locus_tag="PC407_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDF2495783.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            51119..51568
                     /locus_tag="PC407_000302"
     CDS             51119..51568
                     /locus_tag="PC407_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDF2495784.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            51585..52037
                     /gene="rpiB"
                     /locus_tag="PC407_000303"
     CDS             51585..52037
                     /gene="rpiB"
                     /locus_tag="PC407_000303"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2495785.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            52073..52702
                     /gene="upp"
                     /locus_tag="PC407_000304"
     CDS             52073..52702
                     /gene="upp"
                     /locus_tag="PC407_000304"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDF2495786.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            53002..53817
                     /locus_tag="PC407_000305"
     CDS             53002..53817
                     /locus_tag="PC407_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDF2495787.1"
                     /translation="MKNCIAVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            54043..54480
                     /locus_tag="PC407_000306"
     CDS             54043..54480
                     /locus_tag="PC407_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HDF2495788.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            54665..54892
                     /locus_tag="PC407_000307"
     CDS             54665..54892
                     /locus_tag="PC407_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HDF2495789.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            54897..55283
                     /locus_tag="PC407_000308"
     CDS             54897..55283
                     /locus_tag="PC407_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDF2495790.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAIRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            55286..55990
                     /gene="atpB"
                     /locus_tag="PC407_000309"
     CDS             55286..55990
                     /gene="atpB"
                     /locus_tag="PC407_000309"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDF2495791.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            56059..56319
                     /gene="atpE"
                     /locus_tag="PC407_000310"
     CDS             56059..56319
                     /gene="atpE"
                     /locus_tag="PC407_000310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDF2495792.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            56442..56939
                     /gene="atpF"
                     /locus_tag="PC407_000311"
     CDS             56442..56939
                     /gene="atpF"
                     /locus_tag="PC407_000311"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDF2495793.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            56936..57481
                     /locus_tag="PC407_000312"
     CDS             56936..57481
                     /locus_tag="PC407_000312"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDF2495794.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            57498..59000
                     /locus_tag="PC407_000313"
     CDS             57498..59000
                     /locus_tag="PC407_000313"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDF2495795.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            59028..59906
                     /gene="atpG"
                     /locus_tag="PC407_000314"
     CDS             59028..59906
                     /gene="atpG"
                     /locus_tag="PC407_000314"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HDF2495796.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            59919..61313
                     /gene="atpD"
                     /locus_tag="PC407_000315"
     CDS             59919..61313
                     /gene="atpD"
                     /locus_tag="PC407_000315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDF2495797.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            61316..61576
                     /gene="atpC"
                     /locus_tag="PC407_000316"
     CDS             61316..61576
                     /gene="atpC"
                     /locus_tag="PC407_000316"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HDF2495798.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
BASE COUNT        25021 a         6582 c        11484 g        18758 t
ORIGIN      
        1 atattaaata attataaact aaagtaaata aaatattgag cgattattaa aaagatatat
       61 atatatattt taatttgagc agtttgttat atattataaa aaaagagaaa aataaaaggt
      121 aaattgtttc tggataactt aaaattggaa gtaagaatat ttaactagag aagaattatt
      181 taagttcagg ttagctctag gagtagctga ttttatagga gcaatagatt ttatggatga
      241 tgatataaac atgatagcaa ttagaccaga agatataagg gtagaattta atagagattt
      301 caaagaaatt atagacctca aactcagacc gtatcgatat aaaaactaaa gctagatgat
      361 atagtgttgt atataggacc atcagctgtt atggaagtat tagtgctttt aacaaatata
      421 atagtaaaac ttttgtatga attgataata aaaaaattaa aaaccaagac ataaaaatgg
      481 caacaaaaag aagttgaata agggaggtaa aaatgaaatt taagaagtta gtctctttgg
      541 gaatattatc aacactttta ttgagtgtaa taactggatg tagtaatgtt tcatcaggtt
      601 caaacttaaa gtctaataat aagacaaaag gagagttaac agtttataca gcaattgagg
      661 aagatagtat agatacatac ttagctacat ttaaagagaa atatccagat attaaattga
      721 atgtacttag attatcagta ggaggtataa cagcaagatt attagcagaa aaagataatc
      781 ctaaagcaga tgtgttatgg ggtgttgcag ctacaagttt gataatttta gatgacaaag
      841 aaatgctaga gccatacgct ccgaaaggct ttgataaggt aaatcctgca tttaaagatg
      901 ataagaaagt tccttcttgg gttggaatag atgcttggat gactggtata acagtaaata
      961 ctaaagaatt aaaagctaaa aacatatcag ctcctaattc ttatgaagat ttaataaagc
     1021 cagaatataa agatcttata tctatgccta gtccagcttc ttcaggaaca ggatatttaa
     1081 cagtatcaga actcatacaa attatgggtg aagaaaaagc atggcagtat atggataaat
     1141 tgcataaaaa tattggtgta tacactcagt ctggttcagc gccagctata ttagcagctt
     1201 caggagaata tcccattggt atatcttttg gaaatagagg tataaagtta aaaggtgaag
     1261 gttatccagt agaagtagta tttccaaaag aaggttctgg atgggagata gaatcaaatg
     1321 cacttgtgaa aaaagataat ataaaagaag catcaaaagt atttttagat tgggctataa
     1381 gtaaagatgc aatgaatgag tattctaaga attttgcagt tactacaatt gacacgggaa
     1441 atccggtgcc agaaggattt ccaaagaatc cattagaaca gatgatagat aatgatttaa
     1501 aatcagcagc aagaaataga gaggctatac taaataaatg gatagaaaaa tatgatggaa
     1561 agacagaaaa agagagctaa gccttacaat aaaatttagt acaaaaagcg cctagaatat
     1621 aagttttaca tctaggctct tttgatatat ttatttaaaa tgcatatttc tatataaaaa
     1681 taaatagttt agtaccttga ttaaattaaa taaaaacctt aaaataaaga gatgtaaaga
     1741 aaaactaaaa acgaataaaa ggtgaattta tatgataaaa gaaataatag tagtagaggg
     1801 tagagatgac gttacagcag ttaagagagc aatagatgca gaacttataa ctacaggtgg
     1861 atttggattt ccaaaaggtg taatgcagcg tataaaagct gctaatgaaa gacgtggtgt
     1921 tataatattt acagacccag attttgcagg tgaaaaaata agaaaaaaaa tagcatcaga
     1981 agttccagga tgtaaacatg catttctacc aagagaggaa gctaaaaagg atggagatat
     2041 aggtattgag aatgctactc ctcaaagtat aatagcagct ctgaataaag ttagaactga
     2101 aaatacagaa aaaagagatg aatttaagca agttgactta attagaaatg gtcttatagg
     2161 taatgaagaa gcatcactta gaagagatgc acttggaaaa atacttggaa ttggatatgg
     2221 aaatgcaaaa caatttttaa atagattgaa taattatgga gttgaaagag aagaatttat
     2281 agatgcatta aaacaaatct aaacgtaatg tttaatgtat gaaaaatatg tatataaaat
     2341 aaattgtgga attacggtta atttaagata aattatttat aatatatacc ttattttaaa
     2401 tcaatgtaaa tacatataaa taagtataat taataaaatt aaatatcaaa ataagttaaa
     2461 aaggagtaaa taatggatag actatcttca cataatgcaa caaaggaagt agtacaaaaa
     2521 tataatttta aattttcaaa gtctttaggt caaaatttct tgatagatag taatataatt
     2581 gataaaatct tatctggagc tagaatcaca agaggagata atatcataga agtaggacct
     2641 ggtataggaa ctcttactcg tgagatgggt aaaatagcag aaaaagtagt ggctatagaa
     2701 atcgatagaa atttaattcc tatattgaaa gataccttgt cagatttaga taatacagag
     2761 gttgtaaatc aagatatact aaaagtagac atacaggaat tagtaaaaga taaattaaat
     2821 ggtggaccag ttaagcttgt agcaaatctt ccatattata taactactcc aatagttatg
     2881 aaatttttag aggaagacat acctgtaaca gacatagtag tcatggttca aaaagaagtt
     2941 gcagatagaa tgaatgctat tccgggtact aaagattatg gggcattatc tatagctgtt
     3001 caatactatt gtgatacaga gattgtggca aaagctccaa gacatatgtt tataccacaa
     3061 ccaaatgtag attccacagt tataggttta catgtaagag ataaaagaaa atatgatgtt
     3121 cataatgaag acatattttt taaaacagta aaagcatcct ttggacaaag aagaaaaact
     3181 cttcttaatt cgttgggagg tcttggtttt cttaataaag atgaaataag agaaatatta
     3241 aaagaagcta atatagacga aaaaagaagg ggtgaaactc ttagtataga agagttttca
     3301 gttctttcaa atataataaa tacaaaagta tcttctaaat agaaggtact ttttttaatt
     3361 ttgttaacat atattgttta gagaaactgt gttaaggggg gattgcagtg ggttctaaaa
     3421 gaaagtttaa aagagactat ataatactag aagctaaaga cataaacttt agatacaaag
     3481 aacgagtact gccaaaagct tttgcaaaag ttgaagtaaa tgatgaaaaa gcaattattg
     3541 cattatatgt agaaaatcta aaatatgtta aagatgggta taaagcagta gcaataaaag
     3601 atgaccatgg attaatagac ttaggaaata taattttaaa tgaacaagga agaggtgaat
     3661 ttgttttaga tttagaagat tctgatgatg agataaaagg tattgcatta ttgtatgaaa
     3721 gaaatatacc tttaattgga tttaagggaa ataagcttga aaattatgag gacatattat
     3781 ttcaagaggt agaggaagaa gaatatgaag aatatgaaga atatgaagag ttggaagaag
     3841 acgaagagat agaggataat gaggaaagtg atagagacga agatagtgaa gaagaatata
     3901 ttatagaaga atatgatgaa gatgagtatg aagactattg ggaagatgat gaagatattg
     3961 aagaaggaga gttaaaagca agaacggaag tagaagattc tggtgatgag gaaatagaag
     4021 agttcaataa ttataaggaa atagaaaatt ataatagggt taaagtacag gaagaatata
     4081 tagacataga atatgatgag gaagaagagg aagaagaaga agtttttgaa gtaaatagag
     4141 aagaagatag gtttgtttcc tcaaaaccta ctaaatcaaa gaaagttcaa aaagatcaga
     4201 ataatattga aaaaataaaa agagctaatg gagtaagtca aggagagagt ggaaataaac
     4261 aaaccagtac attacttatg ccaagacaaa taaaaaaagg tttgaaatta tttaaagaag
     4321 taaaaccttt tgtaacagat tatatagata agacaagatg gtggaaaata gagataaatc
     4381 ctacaacact gtgtggatat acaatgcctt atttaggata tttaaacgta ttgaattaca
     4441 ctatgtatag tgatgcagtt ttacattcat ataaatacag acactattta tttggtgttc
     4501 agtatgatga atataataag agaaaacatt atatttatgc tatcccagga aatagaaatg
     4561 aacagccaga caatggaagc acaggatttt taagatatca agcttgtgat aatagaacaa
     4621 atagtttagg atattggcta tgttttatag attctagaca aagaaatata gttaggtaaa
     4681 aataataaaa atgaaaaaat ttagactcaa ttcttatttt actgaaaaaa ttaatgaaat
     4741 gtagtacaaa actttaaaaa ctattgataa tatttaaata ttacactaca atatatcttg
     4801 aaatacctat tatattatgg aggaaaatta tgataaacga gcaaagaatt ttaaatgagt
     4861 ttcttgaatt agtacagata gatagtttat ctttaaaaga ggggaatgtt gctaaagtat
     4921 tagttaaaaa attagaagaa ataggttgtt cagtagtaat agacaatgct ggtgaaaaag
     4981 caaatggaga aactggtaat ataatagcta cattaaaagg aaataaagaa ggaaagaaaa
     5041 tattatttag ttcacatatg gatacagtaa ctccaggtat aggggtaaaa cctatagtgg
     5101 atgaagcaaa tggaataata aaaagtgatg gtactacaat attaggttct gatgataaag
     5161 caggtatagc agctatatta gaaggcttaa gatatataaa tgaaaataac atagaacata
     5221 ctgatataca agttgtattc tctatatgtg aggaatgtgg attagttgga gctaaaaatt
     5281 tagactatgg aaaaatagat tctgaatatg catttatatt agatagtggt ggttctccag
     5341 gtgaaataat agtaaaagct cctgcacaag atgtaataaa tgttaagata ttaggaaaga
     5401 ctgctcatgc aggtcttgaa cctgaagctg gaataagtgc tataatggta gctgccagag
     5461 caatagaaaa tatgaattta cttagaatag acgaagaaac aactgctaac ataggaatta
     5521 taaacggtgg aacagctaca aatatagtaa caggagaagt taatatagtt gcagaagcca
     5581 gaagtctaaa agaaaataaa ttagatgttc aaactaaaca catggtagag acttttgaaa
     5641 aagctgcaaa agattttgga gctcaaattg agatagatgt caatagagca tatacaccaa
     5701 tagatgttag tgaggattct gaaataataa aacttgcaaa gaaggctttt tcaaacttag
     5761 gtatagaagg tcatactgaa tctacaggag gaggtagcga tacaaatata ttaagtaaga
     5821 atggtatcga agctataact ttaggaattg gtatgaaaaa tgctcataca ctaagtgaac
     5881 atatagctat aaaagactta tatgactctg ctaaaatggt agtggagata ataaaggaag
     5941 cataaattta aataatagtg tataatatat atatcgccta gcttaagcta ggtgattttt
     6001 cattttatag aagattgata atctctattg aaagcaaaga gtatacattt gcatataaga
     6061 gagtgtttaa tttatgttca gatgtaggtt cttaataata tttagattgt ttcataaggg
     6121 aggaataaat atggaaacaa ggtatgaaga agcaaataaa ataacaatac aatcaatact
     6181 ttggaatgta gtattaacaa taattaaggt tatagctggt gttattggta attctagcgc
     6241 tatgattgca gatggattac attcagcatc agatattata agttctatag gagtactaat
     6301 aggcaattat gtatcttcta gacctggaga tagagagcac aattatggtc atgagaaagc
     6361 agagacatta gtatcatttg tgttatcaat attgcttatt ttcgtttcta taacaatagg
     6421 tatagaggca attaaatctt tatttaactt agatgcatta agtgttccat caatattacc
     6481 attagtagta tcagttattt caatcctgat aaaagagtat caatatagaa taacaataaa
     6541 agttgctaaa aagataaatt caccagcctt aaaagctgat gcttggcatc ataggtcaga
     6601 tgctttatct tcagtagctg cttttatagg tataggtggt tctattttag ggtttaagcc
     6661 actagaccca attgcgtcaa ttgtagtggc tatatttgta gctaaagtag gaattagtat
     6721 acttataagt tctgttaatg aattgatgga tgtttcagtt gacgaagaag agataaaaga
     6781 attaaaattt attgtagcag atacagaagg tgttaaaaat cttggtgata taaagactag
     6841 aaaacatgga gctatggcat atgtagattt gactatatgt gtggatgaaa atttgacagt
     6901 taagcaaggt catgatattg ctaccaagct tgaaaaacat ataataaaac atatggaatt
     6961 tgtaaaaggc ataacagttc acgttgaacc atgtactaat tgccaaggaa ataagtgtaa
     7021 taattaatta aaacttaaaa aaaagtgtaa aaagttatat aattgtttta tgattataaa
     7081 ctctaggggt ggttaaatga gcaagaaaag aaaactaaag aaaaaagctg caatattaat
     7141 aggagtagtt gttttcttta taatatactt tatatccttt agaataggaa taaatatgga
     7201 aaaggataaa agtgaagtgc caaagaaggt agaaaaagaa gaagaagata ataggtcttt
     7261 aaggactcaa ataaaagaaa agaaaaaggt ttatatatca gacaaaaata tagaaaacat
     7321 aaaaattgaa gaagatatgt gggatgaaat aaagttattt ttctcagatt ttaagaaagt
     7381 aagaaatact acttcttctt acgaagcagt ttatgaaggt tattctgatg atggagttaa
     7441 attttctact gaccttaact tttttagagt atatactgta aataaagaag aatattataa
     7501 agtaccagtt agttctaagg atgcttttaa gaaactttta gatgagagta tatatacttc
     7561 atttgacctt ttaagtcatt ataaagattg gaaagaggtt actataactc atggaaaaga
     7621 aattaaaaaa gttcatagat ggaaatttga tgatttagca cataagatga tttcaaagag
     7681 ggttgtagga aaagttcaac ctgagaaaag taaggaaaga agtgactata attttactat
     7741 aaatataaaa ggtgataatt ttgatgtaaa agttgataca atgggaaaag attatgtaaa
     7801 agttacttct aagaaaaatg tatcttacta tgaggttcat acaagtttat ttgattacct
     7861 taaaaatgag atatttgaaa ttggaaagaa ataagacttt aagaaggcta tcaaattaga
     7921 tatttgatag cctaaattat ttttatatat tatttttcaa ccaacaagtc tgcaacctta
     7981 taaattggac cagcaccagc agttattact aaatcattat ccttaacatt ttcacgtaag
     8041 tattttacta tatcttcaaa ttcttttata tagatggcat ctacattgtt ttgatataat
     8101 ttctcaacca agtccttaga atgtatatct ccagggtctt tttccctagc agcatatata
     8161 tctgttatga taactttatc agcagcatag aatgcctctg aaaattcacc taaaagagat
     8221 tttgttctag tataagtatg tggctggaag atacaccata aagttgattt ttttaatttt
     8281 ttggcagcag ctaaagttgc ttttaactca gttggatgat gtgcataatc atctactact
     8341 aaagcattct tataataccc tttagtttca aatcttcttc cgacaccttt ataaattttt
     8401 atattttttc ttatagtctc taagtcaata ccagaaacaa gagctgccat tatagcagaa
     8461 gaagcattgt atatgttatg aagaccatat acagaaagtt caaattctcc taaatctctt
     8521 ccttcatatt ctattctaaa tattccatga ccactattgt caaagtgaat atcttttatt
     8581 acagcatcat tgtcagaatc tctaccgtat tttataatag tagctttaac atcatacaat
     8641 atgtcatcaa tattttcatc atcgccattt attatgaaat agccttctgg gggtaataat
     8701 tttccaaatt tgttaaaaga agccttgatt tcatctattc cagagaaata atcaagatgg
     8761 tcttcttcaa catttaaaac tatagatatc tttgggttaa aattaaggaa actatctaca
     8821 tattcacatg cttcagttat aaagtggttg gaattaccta tcttaacatt accacctatc
     8881 atacttaagt taccaccaac tagtatagtt gggtctaagt ctgcatattc aaagattgta
     8941 gataacatag atgttgtaga agtttttcca tgtgttccag aaactgctat agaattttta
     9001 tattccctca tgatttggcc taaaaaagtc gctctgttca ttaccaattt atttttttct
     9061 ttagcagcca caagttcttc attgtctgga tgaactgctg ctgtataaac aaccatattt
     9121 acatcatctg atatattatc ttttttctga ccaatataaa tagttgcccc ttggtcccta
     9181 agtttgtcta ataggtatga ttcgtttgaa tccgaacctg aaacttgata acctttattg
     9241 atacatattt cagctaatgc actcatgctg ataccgccaa ttcctataaa gtgtatattc
     9301 ataataaaac cacctttctt tttaattaag tcctacaata tgaactattt atgtggtata
     9361 ataatataat gttcaatatt ttataattaa tatgcatata atattatatg tattttattt
     9421 ttgaatataa taaaacataa gtaatattat accacaataa gaattgttat gcattagtta
     9481 gtttaacgtt atataaaggt acttgtagga ggaaataata atgaaattta aaagaacaga
     9541 gagaattgga gcaatagtta aaatactgtc tgataatcca aataaaatat atacattaag
     9601 ttattttaca aatcaattta atgcagcaaa atcaacaata agtgaagatt tattagttgt
     9661 aaaaaatgta tttgagaagc tacatttagg taaagttata actatctcag gagcagcagg
     9721 aggagtaaaa tatataccta aaacttctat agcagaaaat gcagagttct taatggaatt
     9781 atgtgagaga atatgtgaca agtctagaat actatctggt ggttttttat acttgattga
     9841 cctaatttat gaccctacaa tagctgctaa gataggaaag atattcgcat caaatattga
     9901 gtatgtagat gcagattatg ttgtgacaat ggaaacaaaa ggtataccta tggcacttat
     9961 gactgcaaaa gctatgaatt tacctctagt tataataaga aaagatataa aggtatcaga
    10021 aggtcctaca cttagcatga catatgtgag tggaaacagc tctaaagttg aaagtatgag
    10081 tttgccaaga aaagctctta aaccagatag taaagttata attatagatg atttcatgag
    10141 gggtggaggt actataaaag gaatggtaga ccttatgaat gaatttggtg cagaggttat
    10201 aggtactgga gtattcatat ctacaacgaa tccttcagaa aaaatggtaa aagattatat
    10261 ttcattgatt caattggatg ttaatggaga taaaatagta gtagaaccaa acttaaagac
    10321 ttttaaagat gaatatagaa atgaagattt agacgaagaa gaagattcag aatttgaatt
    10381 tgagatagat gaagattaat aaaaaaaata tttagaaaaa tatgtaaaaa taaaaagagg
    10441 atatccctag ttgttcatag aattatttaa aaaacagttt atattaattt attccagcga
    10501 taataatttt aatataaatt aatttctgtc aaaatcaaag ggggatattt tggtatgaag
    10561 ataactgacg taagagtaag aaaattaaca gaagaaggta agatgaaatg tatagtttca
    10621 ataactttcg ataatttgtt tgtagtgcat gatataaaag ttatagaagg ccataatggg
    10681 ctatttatag caatgcctag cagaaaagta ggcgaaggaa atttcagaga tatagctcat
    10741 ccaataaatg cagaaatgag acaagtttta gaagatgcag ttttacaagc atatcatgaa
    10801 gcattagttc aatgggaagt agctgctgaa taataaatat ataaaatgga gagagcccga
    10861 acatttaggc tctttttaaa tgtagaattt ctattaaaat atgtactttt tagtaaaaaa
    10921 gtgctatatt attagtgata atgtatatgt tgtatacaaa ttgttagata aatgtattat
    10981 aatgtatatt gattaagaga ataatacgaa taaaattaaa ttagattgaa tggagtgtat
    11041 caatgaactt taaagctata atccttgctg ctggcaaagg aacaagaatg aagtctaaat
    11101 acccaaaagt tatacataaa gtatgtggaa aagaaatggt taaccatatt atagatgttt
    11161 caaaaaaatc aggggtaaaa gatactgtcg tgattttagg acatgaagct gaggtagtta
    11221 aagaaaagct tgcagaagaa attataatag ccatgcaaac agaacaactt gggacaggac
    11281 atgctgtaaa gatggctaaa gaatatatta atgatgaaga tacaatagtt gtactttgtg
    11341 gagatactcc acttataaaa gaagagacac tgaagagatt atttgaatat catatagaaa
    11401 ataaatatca tgctacagtt cttacaacaa gagttggaaa tccaacaggt tatggtagaa
    11461 taataagaga taagaagggt gaccttctta aaatagtgga acaaaaagat gcaaattcag
    11521 aagaaaagat gataagtgag ataaactctg gaatttattg ttttaatgga aagagtctta
    11581 gagaagcact agatttgcta aataataaca attcacaagg tgaatattat ttaactgata
    11641 ctgctaaaat aatgagagat aaaggattaa aagttggtgc atttgcaggt tcaactattg
    11701 aagaacttat gggcgttaat tcaagagtag aattatcaaa ggcagaagaa attatgagac
    11761 gaagaataaa tgaatctcat atggtaaatg gagtaactat aatagacact aactctacct
    11821 atatagaatc tgatgttatg ataggcaatg atactataat atatccaggt gttatgttac
    11881 aagggaaaac aaggataggt tcagattgta taattggaat gaactcatct ataacaaatt
    11941 cagaaatagg tgatggtaca gaaataaaaa attcaactat aattgatagt aaggttggag
    12001 aaaattctaa tgttgggcca tatgcatact taagaccaaa gagtgactta ggaaataatg
    12061 taaaaatagg cgattttgta gaagtaaaaa atgctattat agaagatgga tcaaaagcat
    12121 ctcacctttc ttatatagga gatgcacatg tcggtaaaaa tgtaaatatt ggatgtggag
    12181 tagtatttgt aaactatgat ggtaaaaata aatttaaatc agtagtaaaa gacaatgcgt
    12241 ttataggttc aaattctaat ttagttgcac cagtagttgt agaagaaaaa ggatatatag
    12301 caacaggttc aacaataaca catgatgtcc cagatggtgc tttggcaata gcaagagaaa
    12361 gacaagttat caaagaagga tgggtagaaa aaaagaatca aaaagacgac caaagtaagt
    12421 aattatgtag tttattattt tggctaaaaa atttagctcg aaattattat aaattatatt
    12481 cattagtatt ttaaaatttt caggagggtt acataaatga atactagcgg aagcgagatt
    12541 aaaattattg caggtaattc atcgaaagag ttagcacaaa aaatagctga ctatataggt
    12601 gtatcagtac tagattgtga agtaggtaca tttagtgatg gtgaaatatg tgtaaacatg
    12661 aatgagacag taagaggttg tgatgtgttt gtagtgcaat ctactaacag cccagtaaat
    12721 gataacttaa tggagttatt aatcttaatt gatgcgttaa agagagcatc agcaggtaga
    12781 ataacagcag tcattcctta ctatggatat gcaagacaag acagaaaggc taaggcaaga
    12841 gatccaatca ctgctaaatt agttgcaaat ttaataactg ctgctggagc agacagagta
    12901 ttaacaatgg atttacatgc agctcaaata caaggatatt ttgatatacc attagaccac
    12961 ttactaggtg gaacaatatt agcaaattat tttaatgaaa agaaaataga agatttggta
    13021 gtagtatctc ctgacttagg aagtgttact agatcaagaa aatttgctaa tactttaaat
    13081 ggagaagttc caatagctat aatagataaa agaagaccga aagctaatgt atgcgaagtt
    13141 atgaacttaa taggtgatgt taaaggtaaa aatgttatac tattagacga tatgatagat
    13201 acagcaggaa caatagttaa tgctgcaaat gcacttaaag agtttggagc aaaagatgtt
    13261 tatgcttgtt gtactcatgg tgtgttatca ggtccagcta ttgaaagaat tgctaattca
    13321 gaaataagtg agttaatagt tcttgatact atacaacttc ctgaagagaa aagaatagat
    13381 aagataaaga taaaaacagt agctccttta tttggagatg ctataagaat gatattctct
    13441 aatgaatcag taagtaaatt attctaattt attgtattta tgagagttgt ttaaaaggat
    13501 ataaaccttt tgagcaactc ttttttgatt ttatacttat ggaatgttat ttttttcttg
    13561 ttagaaaggt atatattatt gtaaaaagag attatagttt aaaattatgt atatttaagt
    13621 aaaataaact taataaaggg catattattc aaaatattat gaatataaaa aatattacaa
    13681 aaaagtattg aaaaaattca gattatgtag tattatgtaa atataaataa agaaagaggt
    13741 gtttccaatg ggaaggttag aagatgaacc cctaataatt aaatgattca gtcacaatct
    13801 aaagtgaata taaaaatcta ttgaaagttt atttataaga attgaaaata gtatctattc
    13861 tatctttagt gtttgtgaga gggttctgga ttatataaga ttactgtaaa gagtaatttt
    13921 gcacaataag catagctgta aatgaagagt ttcgtttata taatgattgt ggctgaatca
    13981 taactataat agatagaatg tttataatag atgattttat aaaatactat attgtaatag
    14041 cttttagctt tcaaataaat attagcaaag gatggaaatg acaatgataa aactaaatga
    14101 taatttataa atatctcctt acgatatata ataattgtag ggagatgttt cttttttttg
    14161 tagagtataa tttttctaga aattctgttt caatatgtaa aaagttggta tatataaata
    14221 gtataaacag tgttgctaaa atatggtaga tattatataa tataagtgga tatgtatata
    14281 cattatgagt tatataattt tgaatttaat aaaatagaaa cgttgattta tgttctgtaa
    14341 tgtgggcacc ttagagcata ttgagttagt ggtgcaaccg gctatgaaat tgtatttatt
    14401 tatagatact atcattttca tagctttttt tatttaaaaa atggttatgt gttagaaata
    14461 aatttggtta gtacgtttaa ataattttac ttagattttt aaccagatat attataagta
    14521 agtattaatt aaaaaattta agggggaata aaaatgaagt taaaaaagaa taaaaaaggt
    14581 ttcactttag tggaattatt ggtagtaatt gcaattatag gtatattagc agtagtggca
    14641 gttccagctt tatttagtaa tataaacaag gctaaggtag caagtgttga gtctgattat
    14701 agttcagtta agagtgcagc attatcttat tattcagata ctaataagat accagttaca
    14761 ccagatggtc aaactggttt aagtgtttta gagacttata tggagtctct gcctgataaa
    14821 gctgatatag gtggaaaata taaattgatt aaagttggta ataaattggt attacagata
    14881 ggtacaaatg atgaaggagt tacattaaca gaagcacaat cagcaaaatt attgagtgat
    14941 ataggtgaaa ataaaatata tacaagtgtt acagcagata atttgggaaa tccattaaca
    15001 agtaatacaa aagtagataa taaagttcta tatatagtac ttatagataa tactgtgatg
    15061 gactcaacaa aatagttaaa actatatttt tataaaaata ttgtaaattt aatgaaaaat
    15121 ataatataaa agacaggaag aaaatcttcc tgttttttta tactattttc tttaatgttc
    15181 aattatgaat gatgatataa ttgagatagt aaaggagtga aacctgtggc taaaaaagtt
    15241 agaataggag acaaactggt cgaaaaagga tatataacag aagaccagct taaatgggca
    15301 ctatcagaac aaaaaaacag tggaaaaaga ctaggagaat ttctagtaca agaagggctt
    15361 atagatagta acttactaat aagtgtatta aaagaactct tggatataga aagtatattt
    15421 ttagaaggga cagaaataga cactttagct acaaagatgg ttccagaaaa tatatgcaaa
    15481 agatatactg tatttccatt taaaattgat ggaaataaaa tatgtctagc catgtcagac
    15541 ccacaagaca gagaagcagt tcaagatgta aggcgtatgt ctggtaaaga cgtagagata
    15601 tttatatcaa gtacagaaga tataaacaaa gcaataggtc atgcttatgc acattcagaa
    15661 ataaataagg caatgactga gtacaacaaa aatagaactg gtggagtaag agaaacagta
    15721 atacttgaag aagatgtaaa tgctgctcca atagtacgat tagtaaataa catactagaa
    15781 aatgctgtta gaatggaagc aagtgatata catatagaac aaagtgaaaa ttacatgcgt
    15841 gttaggttta ggatagatgg aatgttaaga gaatatatga ggatgaactc agcaccatat
    15901 aaggcagtaa tcagccgtat caagataatg tcagatataa atatatctga aaagagaata
    15961 ccacaggatg gtagaatcta tttaaaagta gataataaac ccatagattt tagggtttct
    16021 acaatgccaa ctaacaggga tgaaaagata gctatgaggg ttcttgataa gagtaatttt
    16081 atggttagta aagaagtact aggaatagac gaacatggct ctaagatata tgatgaattg
    16141 ataaacactc cttatggtct tatattggtt gttggtccta ctggaagtgg gaaaaccact
    16201 acattgtatt caatgttaaa tcaactaaat acagaaaata gaaatctatt aacaatagaa
    16261 gaccctatag agtatgaatt acctggtgta aatcaatctc aaataaatga aaaggcaggg
    16321 cttactttcg ccagcggact tagagctttt atgcgtcaag acccagatat aataatggtt
    16381 ggagagataa gagatactga gacagcagaa atagcaatta gagcttctct tacaggacat
    16441 ttagttttaa gtacattaca tgcaaataca gcagttggag caatttcacg tcttttagat
    16501 atggatgtgg aaagtttttt aataacttca tctgttttag gtgtaatatc tcaaagactt
    16561 acacgaaaaa tatgtgagca ttgtaaggtt tcatatgaag cagatattgg agaaaaaaag
    16621 gctcttggta ttgatgtaaa tgaatcagta actatttata gaggaaaagg ttgtgaaagg
    16681 tgtaataata ctggatataa aggacgtcta ggtatatttg aaatgcttga aattactcca
    16741 gaaataaaag agttaataga ttcatctgca aatcaaagag aaattttaaa gatggctaga
    16801 aaacaaggca tggttagctt aaaagaggat atagttaaaa aggttttaaa tggaaagaca
    16861 acagtagaag aaatgattag aataattttg atgactgatt aaaggatgat attatgaaaa
    16921 gttttaaata cacagtaata aaagaggacg gaaaaaagca aactgatgtg attgaagcca
    16981 acgattttaa tgaagctaga aatcttctta gaaagagaaa tcttagagtt attgaaatca
    17041 aagaaagcaa aaataaaaat ataagattat ctagtagaaa gagaaaaaaa gacttgggag
    17101 ctgaccagat aagccacttt tgtagacagt ttgctattat agtatcttct ggtataaata
    17161 gtatttcagg attagaaaca ttagctagaa ggtcaactaa cattacatta agagaagaaa
    17221 ttaatcgtat agtggcagaa ataaaagtag gttctactat tgcagattca atgctttcat
    17281 caaaatcaaa gtttccaaag ttacttggtg ctatggtagc tacaggtgaa gctacaggta
    17341 aattagatga agtattaaaa agtatggctt cattttatgc aagtgaacac agggttaaac
    17401 aaaagcttag aaatgctgca acatatccat taatagtttt gatttcttca tttataatga
    17461 tttttatatt tacaacattt atggtgccta agatgattaa ttcaattaca acagtaggag
    17521 caactcttcc acttataaca agaattgtta tggggtttgg tatgtttatg aagaagtttt
    17581 ggataatagt acttttagtt attatattat ttatctatca gtttaaaaaa tatttaaaaa
    17641 cgtcagttgg aagggctcat aaagatagag ttataaacaa gatacctgtt ttaggaaaag
    17701 gaataaattg tatggtagct acacgatttt caagagcatt gtatctattt gtatcaacag
    17761 gatacccatt agttcaaggg cttgattata ttatagatag tgtaaataac accatggcag
    17821 agaagatttt agcatcagct aaagatggaa taacaagagg agaaggctta gcagaaaatt
    17881 tagagagata tacgtatttt gactctgtgt tggtacagat gatagctata ggggagcaga
    17941 caggggaatt agagaatata agtagacaga tggcagaatt ttatgagtat gaatccgaga
    18001 tttatttaaa taggatggcc tcaatgatag agcctgtttt aataattgca gttggtatta
    18061 tagttgcgat actggttgta tcaatattta tgccaatgct tagtatatat gatgccatgt
    18121 aggagggaaa ggacaaatga ataaggttta tgtatcatat tatggcgatt ctatttccat
    18181 agttgagggc agatataaac acgataagga taagttttat gtaaaaaatt ttaatgtgtt
    18241 ttcaatagag gatattgcac aagatttttc taaggaaaag tatgctctat tgagatacgc
    18301 attagaaaat gttaagttaa aatcaagaca tgtagttttt tgtttaaata caagagatgt
    18361 aattttaaaa ccccaaaaac ttcctaatat ggacaaaaag gatttagatg gatttatgaa
    18421 gctagagatg gacgaaatga tggctttgga gtcagatgaa tatgtatttt cgtatgaagt
    18481 atctagtgaa acacatgata attcagatgg cgatgaatca agcttaaatc ttatcattgg
    18541 agctatacaa aaggaagagg taaatgcaat tgtagagata atgcatgaat ttgatttagt
    18601 attagataga atagatacat tatcaacagc ttacttaaga atgcttaaaa atttagacta
    18661 caatgatata atggtaacca atataagtga caatagtaca attataaata tatataagaa
    18721 agatacttta tttattagtg ataatatacc tataaagata actgctaaag atatggacat
    18781 gcaattatta aatgtagtag aagaatcaaa aggtcttatg aattactatt cgtcaagaaa
    18841 ctttggtaag gttacggata ctatattaat attaggaaaa agatattcca ataaattaat
    18901 ttatgaaact tttagctctg cattttcaag ctatgtttct caaggcctta ctgagataat
    18961 ggatatatct gatatgataa ctggagatat agatgaagat gatataaatt caattgtaga
    19021 gcttttagga agtatgatag aacctaaagg gaaaagagat tttgaaaata taaacttttt
    19081 accaatggat atacttagaa aacagataaa agctaaacaa atgaaaaaca tactgaaaat
    19141 tgctccaata gttattttga ttcttgctat accttattta tgtttaattt tttcaaataa
    19201 tttagcaaat aaggaacttg aaaatgttaa tggtgaaata agaaatattg aatctgcata
    19261 ttatcagatt ggaaatatag aaaaaatgat acagagtaaa agcgaagaaa taaaggtata
    19321 tgatatgtta attgggaaaa aggtaaagtg ggaaaattta ttggatgata tagataaaaa
    19381 tactccaagt aaagttgaac taacaagttt atcaacaact tatcaaaatc ttggaactaa
    19441 taataaagct gataattcaa aaagtaataa gactacaaca gataaatcaa gtaacaatga
    19501 aacaaaatca agtacgaaaa gtgatagtaa tggagataaa acagataatg atttaaatga
    19561 agataagact acaaatgtgt cagaagcatt aactggaaat agtgagacta atagtaataa
    19621 cacggataaa gatgataaaa ctccattgta tgataagata ccaaatgtta taaatataga
    19681 aggaaatgct aaagattcct cttatgtagg tcaattttta tataaattaa agggattata
    19741 ctatttcaag gatgttaaat tacatagcat aaaaagtaat gcagaagatt ctaactatac
    19801 atttagtatg acattatacc tgaaagaagg tgtattaact aatgaataaa gatatattta
    19861 aaaaagatat aaaagatata tttaaaatgg aagttagtct aaaagggatt cttataaagg
    19921 ttataatttt attagctatt ttatcatatg gttattttgg actttaccct caatatcaga
    19981 aatatattgc tacaaaaaat tctgtaaaac aggctactga aaaattaaat atatataaag
    20041 agaaaactgc acttatgcca aaatatgaaa aacaacttga agaagttgaa agtgaattaa
    20101 aagaaaaaag caaaaatctt ttatatgata tgcaagatgg tcttttttta ataggtttag
    20161 ataaaaaaat gaaggcttat aatattgact taactagtta tgacgttgga aatgttaaag
    20221 aatacgaaca tttttatggt atatcaacaa caataagtgt tagaggtgat tatagaaatg
    20281 tcaaaaaatt aatggcttat ttagaagaac aaaaaaatgt tactcaaata ctagattata
    20341 caatgacttc atatatagaa ccagaaaaag aagaggaaga aacaacaaac aataaggatt
    20401 taattaaaaa tgtgaacata aataaagata caaaaatata ctgggtggaa ggcgaaaata
    20461 agtggcattt atataaggat tgtccattga taaaagaagg agagattcta aaaaatgaca
    20521 aacttttaga taggtctact aattatctat gtaatacttg tgtacttaga gctgcaacag
    20581 aaaaagaagc aaaaaaagaa gtcgtagctc caaaggcaga aggtgtagta gatgctacat
    20641 ttaaatttat aatgtacact aaagagaata ctacaatgat acttgataca gataatccat
    20701 cttcatggag accaggaaaa tataatccat ttaagtctag tgctaaataa agctaagtaa
    20761 gctttaaaga taggtgatga gtttgtacaa aaataatgaa aaaggtctta ctttattaga
    20821 agtaataata gctgttttta tactgacaat agttttaagt atttcttata aagtgttcaa
    20881 tggtataaca tcagcagtaa aaaagcaaca gattattaca gatgctcaag taaatattaa
    20941 tctaattaat aagtatctaa atagagattt ggaaaactgt aaagagctaa ctaaaactgg
    21001 ttcaggtaat aattatgaat acaatataga gatgccagat aatgttgtaa aatatgaagt
    21061 ttctatagaa actaaaaaaa atacagaggt atattctgta acgagaatcc aaaacaatac
    21121 aattgataca gaaaatgaag ttagagaaga gataatctat aatcaaccac tagttcaaaa
    21181 caataaagaa atgaaggaaa caccatttaa aatagaaaag caaactggta aatctattta
    21241 tactgtaagt atatactata atgaatcagt gcaagaaagt cataaaaata gcaatttaaa
    21301 taacaaaact tatacatttg atgttatgtc aagaataggg taataagaat ttattaaaaa
    21361 ataagtaggt tgataaaata tgagtaaaaa gaatagtgaa agaggatttt ccttaattga
    21421 agtgttagta gctatggcta ttatgggaat tgtactattt gcatttttta atattatcaa
    21481 tactaataac aaagcaaaca ctaaaaatga cacagatata acttccttaa actatgtcca
    21541 aagtgaaata gaaaatctaa gagaaaagat aaaaagtgga gaatttgatt ttgatagttt
    21601 agataaacta gaagatggaa ctgtcgtata tgaaaaatta atagataaat caaaaaaagt
    21661 tgtatatgat aaagttctta gtgaaggtga cgtgagctta tatgatactc catatgaaaa
    21721 gattacaaca ataaaagatg aagatggtaa tttaatcgac aaaggaaata ttacaaataa
    21781 gataaagact attgtagaag ataaaagtgg tcagatatat aaaatagctg taactgggaa
    21841 aagcatgaat gactattcaa gtaaaaaaga ggttaaaatt gtaactgaaa tatttaaaga
    21901 taaataattt tacttggggg tgagattttg aggaagtgga ataaatttaa aagtgaaaga
    21961 ggggcagctt tagttttagt tttgattgta gtagcattgc tttctattgt tggattgata
    22021 ttttcaaatc agatagctaa tagaataaaa tctacaaaga ctactaatga aggtatacaa
    22081 gcaaagtatt tagcagagac atgtgtggaa aatagtattg ataaagcata tgaaaaattg
    22141 tatgatgagc ttgagaaaat ggataatgag tttaaaagtg aaaatcaaga aaaatccata
    22201 tctaggtcaa aattaagaaa tatatcagat gaagatttta ataatcaaga tgagaaaaat
    22261 attgaagcag aaagacttgg atatatgaat aatataaatt tctatttaaa taaagcttct
    22321 tcagacttgg aaaaagctag tatggaattg aaaaagctat atgatttaga tatgttggac
    22381 tatagagaca ttgaatatgt agatgcaaat attatttcac atagagattc tatcttagaa
    22441 atttgcaaga attatacttc tggagatatt agtaaaataa atgaatatat tctgaaagaa
    22501 gatatagact caactacatt gattgaggca aagttagtga ataatgatat tcttttgaaa
    22561 atgtttttag aagaaaataa aattgaaaat gaacatttaa attcagcctt ttcacatact
    22621 tataaggcat tagataacat ttctttggcg atgcaaaata tgattgagta tagacatacg
    22681 tttcatatag atgagccaaa agtagaagta tctaatggga ttccagattc tcaacaatat
    22741 tatgaactaa tacaaaatcc tataataaat agtatggagt atatctggaa tagtaaatgg
    22801 gatacattag aaaacctatt ggaaatttta ccaaatcaaa ctcaaggatt taattcttta
    22861 agagttcatt tgagaaataa tgtaagaaaa tttgagaagt taagcgataa tatatctagt
    22921 ggtaaaaaaa atacagcaaa aaatttttta aagtataaag agttattgta tgaaatatca
    22981 gaccaatgta atcaactaaa aagtatgtct tatgagaaaa tacctgttaa atatgataat
    23041 atggctttga taacaacttt tgactatata caaaatgagc ttttagcaga gataaaatgc
    23101 agattaaaag aattaaaacc acaagaaatt gataaaacag aaggtattac tataaaaata
    23161 cctttttata aggctgacta cgacatgaca aaagaaggat ggcctaaatt aaaagaaaat
    23221 ggttcagggg cggagttaag cttaatggta actggtgata aagatggaat aaaagaagta
    23281 gaagttacag atggtaaaaa aaatattatt ggtttaggtg ttgaagaaaa ttcaaattca
    23341 aagtataagg ttgacgccat tgtaaacttt aatctaaata tagatacaaa tgtagttgga
    23401 aattatgata taaaagataa aattttgatt aatcatgata taagttcata caaaaaagta
    23461 aactaaatat aagaagcaac ttagtataaa taaataataa aataatttta ttatttacta
    23521 ctaaaaaaat aagtaaacta atagaagata tggagagtaa atattatgta tatttatgat
    23581 ttattagagc aaggtataag actaaacgca tcagatatac acataacagt tggaacaaat
    23641 cctgtagcta gagtaaaagg tggatttgta aaactaagtg aacaaatatt gacatcagaa
    23701 gtaacaatgc aaatggcaaa agacatagcg ggggaatcta tttttaaagt aatagaagaa
    23761 catggagaag ctgatttttc agcttctcta aaaacaggag aaagatttag ggtaaatgca
    23821 tataggcaaa aaggaaatta cgcaatagca ataaggacta taacagcaga aattccaaca
    23881 tttgaaaaat taggccttcc agagagtata aaatctttta ctgaaaagca taaaggatta
    23941 gttttagtta caggtccaac aggaagtggt aaaagtacaa cacttgcaag tatgataaat
    24001 ataataaatg aaaaacaaca aaaacatata attacacttg aagacccgat agaatatgta
    24061 catcatcata aacaaagttt agtaaatcaa agagaagtag gaactgatac agaaagtttt
    24121 cattcagccc ttagagctat tttgcgtcag gacccagatg taatactaat aggagagatg
    24181 agagaccctg aaactgtttc aatagcatta acagcagcag aaacaggtca tttagtattt
    24241 tcaacactac atacagtagg ggcagctaag acaattgata gaatagtaga catgtttcaa
    24301 ccatcccaac agcaacaaat aaaaacacag ttatctacag tttgtgaagg tgtggtatca
    24361 cagcagttac ttcctacagc agatgggaaa ggcagaatag cagcaattga attgatgttt
    24421 gctactccag caataaaaaa cttgataagg gaaggcaaaa catatcaaat tccaaacatg
    24481 atacaaactg gagttaaagt aggtatgaaa actatggacc aagatttaat ggaactttat
    24541 aaaaatggaa agattacaaa agatatggca cttagtaggt gtactgacca agagtttatg
    24601 acaagaatga taggtggagt aaactacaat ggatattata atcgttaatc tatatgtatt
    24661 tatagtgggt atagtgtttg gaagtttctt taacgtctgt atttttagga ttccagaagg
    24721 tatatctatt tctagacctc cttcccattg tatgagttgt aatactagac taaagccaat
    24781 agacttaata ccagtgctaa gctatttgat gagtggcaga aagtgtagat attgttatga
    24841 gaaaatttct agtagatatg ctatagtgga attaattaca ggaattttat ttttaacagt
    24901 atttatgtta tatggaatat ctacatcaac tatttattat ttggttcttg tgtctttact
    24961 gattataatt acatttatag atattgacca ctttataata ccagataaaa tattgatttt
    25021 tggagctata ttttctatga tttttaacct tttatttaaa gaaattccag ttaaggattc
    25081 aattctaggt gtttttattt gtggaggaag tgtctggatt atagttcttt tgattgaatt
    25141 tgtaataaaa aaagagtgta tgggtggagg agatatcaaa ctatttgcaa tgctaggcct
    25201 ttacatgggt gttaaaaatg gtcttttgac agccttacta agcgtttatg taggagcagt
    25261 ctatggaatc tgtgtaataa ttataagtag aataaaagga aaggaatata attctgttat
    25321 accatatgga ccttttatat ctattggagc attaataaca atcttatgtg gaaagcaatt
    25381 acttgaactt tatttttcta tggttatata gatgatagtc aagttaaata aagttttaat
    25441 ttaaaaaaat gaattaaagt atttaataag gacaatatta ttattaaccc tattactaaa
    25501 gtgctacaaa ctatggagta tataataata atttccttat tttttgcaca tttgataaat
    25561 aaatctctat gtgttttttg tgatagagaa aagaaaaatt tatataatac gttttttatt
    25621 tacataataa ctgtagtttt atctgtatta atatatctaa aatttgggat tagtgtagaa
    25681 tgtgtacaat atatggtttt aattccattt ttaataatta tatccattgt agattactac
    25741 acaatatatg tatatgatat aacaatagtt agtggtataa tagttcaagg tataattttt
    25801 ttggcctttt tcaatatagc tcaatttgat tatataaatc atatatttgg actattgata
    25861 ggctttatta taccttatat tattgctaaa ctaacaaaag gattaggcta tggagatgta
    25921 ggtctctata gcttatgttg ctttgcattg ggaaataatt atgggtttta tgtgatagtt
    25981 ttatcattta ttttagcgtt tttgtttttt attattttat acataactaa aatatttaaa
    26041 gatgtagata taagaaagca aattcctttt acacctttta tttccttgtc aacaatttta
    26101 ataatattta cagagtataa tatatttaat ttttattata atttgttata caaatttata
    26161 taacaggagg taagaaatgt acgtagtagt agggttaggt aatccaggaa agaagtatga
    26221 gaagactaga cataacgttg gatttgatgt tatagatatt cttgctaaag aatacaatat
    26281 tagtgttact aaaataaagc ataaagcttt gattggtgaa gggagagtag gtactgaaaa
    26341 agtattatta gttaaaccac aaacttatat gaatttaagt ggagaaacat taatagatat
    26401 atataaatat tataaggttg atttaagtaa tatagtagta gtatatgatg atatagattt
    26461 agaagttgga aagatacgta taagaaaaaa aggaagtgga ggaactcata atggcatgaa
    26521 atctataact aagtgcctag gttcaaatga ctttccaaga gtgagagtag gtgtatctaa
    26581 accagaagct ggtcaagact tagcagactt tgtactttct agatttagaa aagaagaatc
    26641 tgataacata aatgaagcat tagaaaaagc ggcagatgct attgactcaa taataagaga
    26701 aaatatagat atgtctatga acaaatataa tggatagtga gagggagatt taaatgaatg
    26761 atgttttttt atatcctttg caaaattcaa aagagtataa agatataata aactgtataa
    26821 aaaatacaaa aggctcatta ttggttaatg gacttttacc agtacaaaaa cctcatatat
    26881 cgtattctat atttaatgat ttatcacgac aaatgatttt tataacgagt agtgatttag
    26941 aagcaaagaa agtatacgaa gatttaagtt tttatatgga agataaagtt gaatatttag
    27001 gttttcaaga tatttatttt tatcacttgg atgcaaagga cagaaacgaa gaagcaaaga
    27061 aattaaaagt attgttaaaa ctagcaaata aagaaaaaat cattttagta acttctattg
    27121 aggcagtttt aagaaaatac ataccaaaac aagttttgct cgatagtgta tctcattata
    27181 aggtgggaga tagcttagac ttagaaaagt taactgagaa attagtttca ttgggatatg
    27241 aaagagtttc aaaaatagag gggtttggtc aatttagtat aagaggtgga attattgatg
    27301 tattttcact tgaatataca aatccaattc gtatggaatt atttgatgat gagatagatt
    27361 ccatacgaac ttttgacgta tattcacaaa aatcaattga taaattgcaa caattctcta
    27421 taactccatc tagagaattt atttatcccg aaaaaactac agatgcatta gttaaattaa
    27481 aaaaagaaac aactaaaaat actgatgaag gtacatttca aaatatagat tatatatcaa
    27541 gtaagactta ttttgaaggt gcagagaact atatagatta tatttatcca gaagaaaaca
    27601 aaagtatatt tacttattta gcagatgatg caatagtatt tattaatgat ataacgagac
    27661 ttaaagaaag atgcgaaaat tatataaatg agtttagaga aaattataaa ttaaatcttg
    27721 aaagaggtct ggctattaaa aaccaaggta aattgttata tcactataca gatttagagt
    27781 acttagtaaa agacaagtct gtggtattaa actcgctatt gccaaagtct ataaataact
    27841 tttctataaa atctataata aattttgaaa gtagagaagt tcctacattt aatggaaagg
    27901 tagatttgct tgtagaagaa ttaaatcggc tgaaatataa tggatacaaa ataattttag
    27961 caacaaatac cttagaaaga gcaaataaat taggaaaaga tttattggac aaaggcttag
    28021 aaacgaccat ttctaaagat agagatattg aaattaaatc ttcacaagtt ataattgtac
    28081 ctgcacacat aaacagtggt tttcagtata aatctataaa gtttgttgtt ataacagata
    28141 acgaaatgat aggtgtttat aaaagagcgt ctaagactag caataaaaaa gttaaaaaag
    28201 gaaagaaaat agaatccttt ttagatttaa gtgttggaga ctatgtagtt catgaaaata
    28261 gtggtgtagg tagatacaca gggattgaac aaattacagt caatgctatt aaaaaagatt
    28321 atatgaaaat agtatatcaa ggtggagata atctatatgt gccaatagac cagatggaca
    28381 aagtacaaaa atatattggt gcagaagtag aaaaagttaa gttaaataag cttggaacaa
    28441 atgagtggac gaaagcaaaa gcaaaagtta aaaaagaaat tgaagatatg acaaaagact
    28501 tgattgagct ttatgcaaaa cgtgaaaaaa tacaagggta taagttttca aaagatacac
    28561 cttggcaggc agagtttgaa tcgttgtttc catatcaaga aacagaagac caattaaagg
    28621 ctatagaaga aactaaaaaa gatatggaat caagtaaggt aatggatagg ctagtatgtg
    28681 gagatgttgg ttatggaaaa acagaggtag ctattagaag tatttttaaa gcttgtatgg
    28741 accaaaaaca agttgctgta ttggttccaa caacaatatt ggcccaacaa cattataata
    28801 cattcaaaga gaggtttgaa aattatcctt tgagagtaga agttttaagt aggtttaaaa
    28861 caccaaaaca acaaaaacag attattgaag atgctaaaaa aggattagta gatattttaa
    28921 ttggaacaca tagaataata tccaaagata taaatttacc aaacttaggt cttgtagtca
    28981 ttgatgaaga acaaagattt ggagtaaaac ataaagaagc tttaaagaaa attaaatcta
    29041 cagtagatgt acttacactt tcagcaactc ctataccaag aacattacat atgtctttaa
    29101 gtggaataag agacatgagt gttatagaag aacctccaca agaaagacat cctgtaataa
    29161 catatgttac agagagtaag gaaagcgtaa ttcaagatga gatagagaga gaattaagta
    29221 gaggtggaca ggtatttttt gtatacaata gagttgaaca cattgaagaa atggcaagta
    29281 tgattcaaaa actcgtacca gatgctagag tggctgttgc acatggaaga atgacttcta
    29341 aatcattaga aaatataatc ctaggatttt taaataaaga ttatgatgta ctagtctgta
    29401 ctacaataat tgaaactggt atggacatat caaatgcaaa tacaatgatt atatatgatg
    29461 cagataaaat gggattggct caactttacc aattgagagg tagggttgga agaagttcaa
    29521 gacaaggata tgcatatctt ttgtatgaaa aagataaaac tttaagtgag atagcggaga
    29581 agagattaaa agctatcaga gaatttacag agtttggttc tggttttaaa atagcaatga
    29641 gagacctcga aataagagga gcaggaaata ttttaggttc tcaacaacat ggtcatatgg
    29701 cagttatagg ttatgatttg tacgttaaaa tgcttaatga tgcaattaaa aaagtcaaag
    29761 gtgaacctat tgtggaggaa attgatgttg aaatagattt aagtgtaaat gcatacattc
    29821 cagataatta tatcaaagat gagttgatta aaattgaaat gtataagaaa atagcttcta
    29881 ttgaaaacaa agaagacatg ttggatatac aagaagaact tgaggataga ttctctgata
    29941 tacctaaacc agtccagaca cttcttacaa tagcttatat aaaatcgtta tgtaagatat
    30001 taaaaataga aaagataaga caattaaaag atgaaatatt attagttcca ataacaaagt
    30061 atagaactaa acagaaaata ggttataata tagttacaga attagaagaa ttattagaaa
    30121 agatgtgcaa ggtaaaataa cttgtaaatt taagaaggga tgttttaaat ttgaaaaaag
    30181 taataacatt ggtaatagct atgattttgg tagtatctgt gactgcttgc agttctagta
    30241 aaggagaaac tgtggcaaca gttgaaggta caaagatatc atcagatgaa tttaaaaaaa
    30301 ctatagctct ttataaagat tctatggaac aaacttatgg aaaagatata tgggataagg
    30361 aagttgaaaa gggtgtaaaa tacaaggaca agtttaaaga tttaattctt gaccaactta
    30421 taactacaga agtaatatat tctcaagcta aaaaagataa cttacttcct aaaaaagagg
    30481 atgttgaaaa aagttttaaa gaattaaaag acgctatggg taaagatgaa aaatataaag
    30541 aacaacttaa gaaattaggt atagatgatg agtttttaag agaccaacaa gagaaagatt
    30601 tagcaatgca aaactatcaa agtaactttg ctaaaaagac taaaatatca gatgaagaaa
    30661 tgaaaaaata ctatgataca cataaagatg aatttaaaaa agatgaggta gaagcttctc
    30721 atattttatt gaaaacagta gatgataata acaagccact ttcagataag gaaaaagctg
    30781 aagctaagaa aaaggctgaa gaggctctta aagaagttaa atcaggtgag gattttgcaa
    30841 aagtagcaaa aaaatattca caagatactt cagcaagtga tggtggtaaa ttaggattct
    30901 tttcaagggg gcaaatggtt gctgaatttg aagatgctgc tttttctatg aaaaagggtg
    30961 aagtatctga tttagttgaa actcaatatg gataccacat aattaaagtg acagacagaa
    31021 taaatgagca aacatcattt gaagatgcta aagaaactat aaaagaccaa ctgttgaaaa
    31081 ataaatatca agaacaaata gaaaaattaa ctaaagaagc taaggtagaa aaagatgaaa
    31141 aagtaataaa taaaatcaca atctaagact taaaaaaaga gtaatatgct taaaaaagct
    31201 atatctggat aagatatagc tttttttgta tatttttctt aacaatggta aaaataaact
    31261 taaagtaatt tatggaggga ttaaaaaata tgaaagcaac aggtatagtt agaagaatag
    31321 atgatcttgg aagggtagtt atcccaaaag agataagaaa gacattaaga attagagaag
    31381 gagacccttt agagatattc acagcaaaag atggagaagt catacttaaa aaatactctc
    31441 caataggtga attaaatgaa ttttcgcaag aatatgctga aacattagct gaagtactag
    31501 gatatggagt gttgataaca gacttagatt ctataatagc tgtgtctaag cttcctaaaa
    31561 aagattataa agaaaaaagt ataagtgatg agttagaagc aataataagt gaaagaacag
    31621 taaaatattc aaaagataaa aacattttac cattattcaa ggaagaccaa aaagaatata
    31681 ctagtcaagt tatcatgcct ataattagtg catctggtga ctgtataggt tcaattgtag
    31741 ttgtttcaaa ggataaagat acaattgcag aaagtgaaga aaaatcttta aaaattgctg
    31801 ctaacttttt aggaaaacaa gttcaataat tttatcataa agacctttta catgaaggtc
    31861 ttttgaagtt tttgtgtaaa atattgaata tagcattaaa aatatggtat attaaagttt
    31921 gttatataaa cagtatggag gttatgttat gaacaataac aaaaataaag actcctttct
    31981 aaaaggagcc cttatacttg ggctagcagg tgtaatcgta aaaataatgg gtggattttt
    32041 taggattccc cttggaaaca tgattggttc agaaggaatg ggatactatc aggctgctta
    32101 tcctgtgtat actctatttt taacattagc tactgctgga tttccaacag ctgtggcaaa
    32161 attggtttcg gaaaaagttg caattggaaa ttttaaaggt gcaaataaga tatttaaggt
    32221 atcacataca gttttattca ttaccggtat aatatcattt tgtatattat tttttggagc
    32281 cgactacata gtaacaaacg taatgaaaaa tccaggtgcg ttatactcta tgaaagccat
    32341 tgctccagca ttactatttg ttcctgcaat gtctgcatat agaggatatt ttcaagggag
    32401 acaagatatg actaagattg ctgtgtctca agttgcagag caatttttta gagttgtatt
    32461 aggtcttact ttggcctact tgcttatgaa gagtctaggt cagacttttg gtgcagcagg
    32521 tgctatatca ggggctacaa taggttcaat agcatctatg ctttatttgg tgtttgctta
    32581 tatgctaggg agaaaagagc gtagagcaga gatagatgca agccaaagat ttaaagatga
    32641 aagagtgtct tatattttta aaaagctttt aactgttgcc attcctataa caataggagc
    32701 atcagtaatg ccacttgtaa atatgataga taatgttatt gtaataagaa gacttatgga
    32761 agcaggattt acatacaaag tggcaaattc aatgtttggt caattaacag gtatggcaat
    32821 ggccacaata aatttaccag cagtcataac aactgcaatg agtatgagct tagttccagc
    32881 aatttctaaa gcatatgcat taggaaataa gtcaaaagct agaaaagata ctaagagtgc
    32941 agttaaggta actttactta tagtattgcc ttgtgcgttt ggtatggctt ctttagcaat
    33001 accaataatg ggtcttttat ttccacatga accatcttct gtaggaacta ttttgtttac
    33061 tcttactcca tgtgtattat ttttaggatt aatacagaca ttgactggta taattcaggg
    33121 aatgggaaaa ccaatagtac ctgtaattgc tttatgtgtt ggtatgctat gtaaaattgt
    33181 aataagttat actttaacag gtataccaga tattaatgta ctgggttcag catttggtac
    33241 agttacagca tactttgtgg cagccatgat taacttacta tatgttaaaa aacacatgaa
    33301 tgtaaacttt tctaaaaaag aatttataat aaagccattt ataacagtta tgacaatgtt
    33361 tataatggtt aagttgagtt atggtgcttt agttggattc ttaggaaaca gcatatctac
    33421 aataattgca atatgtgttg gtggaattgt atatgtagta gtaatacttg gaataggtgg
    33481 aattaaaaaa gaagagattc taactatgcc aaaaggtgat aaattatata aattgttaaa
    33541 aaaagttaaa cttataagat aatataggaa tatatatgca aataaaggaa atactataca
    33601 aatagtaaat ctccttattt gtataataat ataaaaataa agtgaaattt gctaatagga
    33661 gtgatgttat gggtaagata agtattgtag gtcttggacc aggagactat tctcttataa
    33721 gccaaggtgt gttagaatct ttaataaata gccctaaagt tttttttaga acagagaaac
    33781 atccaataat tgataaactt aaagaaacta tacaatacac ttctttagac tacttttatg
    33841 aaaaagagga aaattttgat aatgtttatt tgaaaatatc tgaatttatc atagaagaat
    33901 cagcaaaggg tgacctagtg tatgctgttc ctggtcatcc aagagttgca gagaagactg
    33961 taagtataat agagagatta gccaatgaaa agcacatagc agtagaaata ttgccttcta
    34021 tgagctttgt agacgctatg tttaactatt tggctataga cccatctgag ggatttaaac
    34081 tggtagatgc ttttgaaata gaaaattcat atatagactt ggatacaagc atgataataa
    34141 ctcaggtata tgataaattt atagcatcta atgtaaagtt aaaacttatg gaacattatg
    34201 actataatca agaagtatgt atagtaaatg gagcaggtgt caaagattta gaaagtaaaa
    34261 agtttataaa gctttgtgaa ttagacagaa atgaaaactt ttttgactat ttaactagct
    34321 tatatatacc caaaagttct aaaaagatgt ataatacagt acatgactta gaaataatag
    34381 ttaatacact tagaagtcca tctggatgtg aatgggataa aaaacaaaca catcaatcat
    34441 taaaaaattc tgttatagag gaagcatatg aactttgtaa tgcaattgat aataatgata
    34501 tagatgaaat ggtagaagag ttaggtgatg ttcttttaca ggttatattc cattgtcaga
    34561 taggaaatga ggagggctac tttgatttaa aagaagtagt aaatggcata tgtaaaaaat
    34621 taattcatag acatcctcat atatttagtg gtgcaaaatt agatatgtct aagtttgaga
    34681 aaacttggga agaattaaag aaagaagaga aaggcgaaag tactataaca gatggcctta
    34741 gaaggatacc aaaacattta ccagctctta tgaaggctag taaagttcaa cataaagcag
    34801 cacttgtcgg ctttgattgg gacaatgtag aagatgtatt taaaaaaatt gaagaagaat
    34861 atcaggaatt acttgacgaa cacagggtcg ggaatataaa atacataaag gaagagctag
    34921 gcgatttatt attttcgata gtaaatctag ctagattttt agacatagac tcaggagaag
    34981 ccttgaactg tactacagaa aaattcataa atagatttga ctttatagaa aaaagtgcaa
    35041 tgaacttgaa taaaaaatta gaagacatga cacttgaaga gatggatgag ttttggaatc
    35101 aggctaaaaa acaataaaat tgtggaagga aatttgtcaa aaatctagaa cttttatcac
    35161 aaagatagaa gtctatatta taaacatttt taggaggtaa atatcgtgaa taaagctgaa
    35221 ttagtatcaa agatggcaga aaaaagtgga ttaacaaaga aggaagcaga agctgcgtta
    35281 aacgcattta tgagttctgt tcaagatgca ctagtaaata atgaaaaagt tcaattagtt
    35341 ggatttggaa catttgagac aagagaaaga gctgctagac aaggaagaaa tccaagagat
    35401 ccagagcaag ttatagatat accagcttct aaagcaccag ttttcaaagc tggaaaagga
    35461 ttaaaggata taataaatgg ataattagaa atcttgtgaa aaagtgtgga taaatttgcc
    35521 acactttttc tatagaacca aggaaggtga taattatgag attagataaa tttttaaaag
    35581 tatctaggat tataaaaaga agaactgttg ctaaagatgc atgtgataaa gggattgtca
    35641 ctataaatgg aaaagttgct aaatcttctt cagaggtgaa tattggagat acattagaaa
    35701 tacaatttgg agagaagaag atgaagttta agataaatga agtaaaagag catgtattaa
    35761 aaaatgatgc aaaagaaatg tacgaaattt tagaataaaa ttaatagtcc tctagtatat
    35821 taatagtagt attattgaaa tatttactgg ggggatatta tggaacagaa cataactttg
    35881 aaagatagat caaagctggt tatttcggga gtcgagcaca tatactcttt taatgacaaa
    35941 agagtagagc tcaaaacttc tgtaggcgag atggtaattg aaggcgaaaa cctagatatg
    36001 agcaagctaa gtatagacga aaatataatt agcatagatg gaactataaa ttctatggta
    36061 tatgcaaagc ctaaaaagcc acaagagagt ttcttgaaaa aggtatttag atagatgata
    36121 ccttttactc aagatgtaag cattttttat gctactattt atggtggaat actcataggg
    36181 gtgttgtttg atttttatag agggctaaga gggaatttta aattcatcaa ttattttgct
    36241 ataatctttg atgtattgtt ttggtttcta gctacggtta taatattcgt aacaataaac
    36301 cttacagaat tttttgattt aagatattat cattttgtgg ctttgtttat tggttttatt
    36361 ttatactata acaccataag taaaattgtt ttatcaatta tcaataaaat aattcgtttt
    36421 gtgagaaatt cttttaaaaa agttacacat tacatagtca gttttttaaa taatttgtat
    36481 tacgttataa tctattcact acacctacta tttgatatta tattttatat accaaacata
    36541 tttatagcaa caagaaaaag tataaaaaga aaatcaaata aaaaattaaa gaacaagaaa
    36601 aaatcaaaac ctaagaagaa aaaaaataaa actaaaaaaa gggtgtagga aaaatgaact
    36661 taagaaaaaa attttcagga cagattattg ttatcagttt atttttagga atatcaattt
    36721 ttagtatgat gacaggattt gtatttgagt atacaaaagc taaggaatat aaaaaagaaa
    36781 tagcaagtct aaataaacaa ttaaaaaaga ctgaaataca aataaacagc ttaaagaaag
    36841 atgaaaaatc atatgaagga gacttagagg atatagcaag aaaaagattg aatatggtaa
    36901 aaccaaatga aacagtttat gtagacataa atcgttgatt aaaacttgct aataagttta
    36961 cagatgtatt gattttaaaa tttaagattt aataaaaaag tcattcagtt aaggatggct
    37021 ttttgttttt attgaggagg gaatttcata atgaaagtag gagctataga tattgggaca
    37081 aactcaatga gattactgat agcagattat agagatggga attttataaa cagaaaaaaa
    37141 tttgttaata caactagaat cggttatggt gtggatagta aaggatatat aaaagaagat
    37201 gctatcaaaa gaaatataaa agcattaaaa gaattttctg ataagtgtaa tgatgaaaag
    37261 tgtgaaaaaa tttattgtat ggggacatct gctcttagag acagtaaaaa tggagattac
    37321 tttgttaaat tagctaaaga agaaactgga atagatgttg atatagttac gggaatagaa
    37381 gaatcaaatt tgggctttaa gggtgtttta gaaggaatac aaggtgatga tactattatt
    37441 gttatagata ttggaggagg ttcaacagaa tttattgtag gtgattcaga aggaattaaa
    37501 tttaataaaa gtgagaatgt aggtgctctt aggatgacag agaggttttt gaagcacgat
    37561 attattgaag atgaagagtt tgaagctatg agttcattta tatatgatga gattgacaaa
    37621 acgttaaatc atataaaaga cagaggatta aaaactctta taggtatagg aggtacgatt
    37681 acatcattat cagcaatgaa tcaagaatta gagatttact ctatggaaaa aatacataat
    37741 agtaaaatac acaaaaaaga aatagaagaa attctacaaa aattaataaa gatgacatta
    37801 agtgataaaa agaacttaaa agggctacaa gaaaaacgtg cggatattat aactgctggt
    37861 gtaaaaatat tgaatataat aatggaaaaa ttagaaatag aagaaataac cgtaagtgaa
    37921 tacgataatt tggaaggctt aatatgtcaa aagtcaaaaa aaatgtatta atattttttg
    37981 atttttaaaa ccgattatga caaaaaaaat tactcctctt tgttacaatt cttaatataa
    38041 acaaaagagg aggttttttt atgcaaagaa gtgtagcagt tgtgaagaat aagactgtaa
    38101 aaccaaatgt cataaaatta ataagcggat atattgattt tactacggcg gcattcgctg
    38161 tgatgggatt tttacttagt aggtctatta tagtagactc tgtagctcct ttaggaattg
    38221 ccttttttat atgtgcatca aagattgata agtacaagat acctgtattc atatcaacat
    38281 tattaggaat attgttatca tctaatgatt ttggaaatgt agcaaaatat gctgtgtgcc
    38341 ttacaatatt tatggctatg aataaaaaat taaaattatc agattcaatt gctaaggttg
    38401 catttatagg tgcaataata ctacttccaa tctccatggg acaagctttc ttttcaaata
    38461 ggtatgtata tggtcttctt atagcaggag tagaagcaat aataatgttt atatctatat
    38521 atgtgttttc ttatgggata ggtttaatgg ccaatgtcaa caataggatg tctgtacgga
    38581 cagaagaagc tatatcaata agtttactgc taacctttag cataatggga ataggagata
    38641 tatctctgtt tggaatatca ataaggacca ttttatcgac cgtattgata ttgctgacct
    38701 caatagtagg tggagctacc atgggagcat ctagtggtgt aattattggt gtagcatcca
    38761 ttttaaataa tatgacatca gctgtttata tggggatata ttctttttca ggattaataa
    38821 gtggagcatt taataaaata aataaatatt tctgtatact aggatatatt ctaagttgga
    38881 caataatata cctatatact tctggtatta cttctaatat gatgcaatta agagatatac
    38941 tattaggttg tttgatagta ctagtattgc cagagagatt atttaataag attgaaaaat
    39001 taataaaaag taatgtggct tcaaatgaaa tcgtatacga ttatattatg agaagcaaaa
    39061 acctaactaa tagcagattg aacagtattt ataaaactta tgatgacctt gcagatacat
    39121 ttgataagat aagagaaaaa gacaaagtgc tagaccaaag agatatagct aatgtaatag
    39181 atatgattca taatgatgaa tgtaaaagtt gtagtatgag aagaatgtgc tgggagtcaa
    39241 gatttaatca tacatacact atgatatata acattcttga aaaaattgag gagaaagggg
    39301 agttaagtct aaacgatata cctaaaaatt ttagaaagga atgtatgaaa ccagagtcga
    39361 tagtgaaaat atcaaatcac tattacaaaa tgtttgtatt agattatgat tggagtgtga
    39421 agttttcaga aagtagaaag ttaatagcaa atcagataag aagtatttca aaatccatta
    39481 aatccttatc tcaagattta gaaggtgaca taatgctaga tatagaaaag gaaaaaaata
    39541 tatatgaaca gttagaaaga tatgatataa cagttgataa agtaagttac ttaacaaaaa
    39601 gtaacaatga gtttgaaata tcaatagaaa agaaaacttg tcatgatgga tgtatgtgtg
    39661 aagataaaat tgtaaatata atttcagacc tagtaggtga aaatatgtct gttagaaaaa
    39721 taggatgtca ctgtttagga ggtaaatgca aggcaacatt cgttaaatct caaaaatata
    39781 aggctgttac agaagtttca gcaatgtcaa gggatgggca tatcctttgt ggagacaatt
    39841 acacatacat ggagataaat gatggtaaat acatgatggc tataagtgat ggtatgggaa
    39901 agggtaaaaa agcatatgaa gaatcttctg ccactattga tatactggaa aaaatgatag
    39961 atgcaaaaat taaagatgag atagtcattg atactataaa taatatgttg ttgttaaaat
    40021 cttcagaaga gatgttttca actctggatt tgggaatttt agatttaaaa cgaggttgtt
    40081 tagaaactat aaagatggga gcgtgttcta cttatataaa aagagaggat ggagaagttg
    40141 acttaatatc atcatcatca ttacctgtgg gtatattatc agatgtaaaa attgatagaa
    40201 aaaatgtaaa agttaaggaa ggagactatg ttataatggt ttctgatgga attgtagatg
    40261 caggaagaaa taacaattta ggagacaatt ggttaatata cttcttaaag aatatagaga
    40321 ctacaaatcc aaaggaaatt tcaaatttaa tattggatag agcattagag ttacaagcgt
    40381 tacaaatcga agatgacatg actgttttag ttacgaaaat atgtacaaaa taaggtttac
    40441 aagattatgt gtataagatg tggataactt gtgtataata tgttaataga cttttgaaaa
    40501 aagtatagtt attaacaaaa ataacatata aatcaacaaa atttgttgat aagtatggca
    40561 gaaatgttaa taaaatgtaa aaaaagaaaa aacaaaagct attatcaaaa gagattcatg
    40621 ttgaaataac taaatttcta agtaaagaaa acctgtgaat atgttgatta ttatgtggat
    40681 aactattaat tcgatattta tttatatagt atgaataatt gaataatata tctaaaaaaa
    40741 gccatcctat tattaagtaa atttattgaa aggatggctt tttatgaata gaaaattgtg
    40801 caaagcaata atacctttat ttatttctat tttttgcatg ttaataataa gtataaaagc
    40861 tgaccaagga tatcaaaaga ataatgacag agcaattgct gtcatgaata ataatgatac
    40921 tgaagatgat gctgaaaaaa aacaagaagt agactatgaa aaaacatgtt ggaatttgca
    40981 atctttgttc aaaagtgatg accaatggaa aaaagaactt aagagttttg ataaagatac
    41041 taaagagttg aaaaattaca taggtaaagt aacaaaatcg ccaacacatt tgtcttttgc
    41101 cttaaacatt aaggaaaaac ttgatataag actaaataaa ctttctgcct atgtaaaatt
    41161 aaaacaagat actaataaaa attcatataa gtacttagat atgagtgata gtatgagtaa
    41221 atcatatggg aattatatgg ggatttgttc agatttagaa ttagaaatac taaaattatc
    41281 aaacaaagat tacaagaaaa ttatttcaaa taaaaatata aataggaaat atggtgcata
    41341 tttaagagat ataagaagga ataaagtaca ttatttggat gataaatcag aagatattct
    41401 aagcaaagta tcttcaataa gtagtcttcc aagccaagta tatgaattat ttagaaatat
    41461 ggatagaaaa actaatttga caccagcgca atatgcaagt gaattagagt cagtagatag
    41521 agaaaataga aaaaaagctt atcaagatga acttataact tacaatgata atataaatac
    41581 aattgcaggt ttaattactg gtcaagttaa aaagaatatt ttttattctc aggaaagagg
    41641 ttatgagtct tctttagaaa tgtacttgga atctgataat gtagatgaaa aagtatacaa
    41701 taatttgata aggactgtaa atagtaatat ggacagtcta cacaaatata tagatttaag
    41761 aaaaaaagta ttaaaattag ataaggttca ttcatatgat atgtttgtgc ctatggttaa
    41821 ggctgttgat aataatatta attatgaaaa ggcacaaagt atagtatatt cagcgttgtc
    41881 acctttagga aaagaataca atgatgtagt atataaagct ttcaatgaaa aatggataga
    41941 tgtttattca catgaaaata aagtaagtgg tggatactgc ttatctgttt atgaaaatca
    42001 tccatatata cttttaaatt ataataattc attaggttca gtttcgactt tatcacatga
    42061 gcttggacat gctgtatatg aatacttaag ttctaagaat cagaattatt ttaattcaaa
    42121 tccgtctata tttactcacg aagttgcatc tacaacgaat gaagcattac tttacgagat
    42181 gcttataaaa gaagctaaag gcaatgatga aaaagcttat tatataacac agtatttaga
    42241 cttgataaaa gatactcttt atacacaaac tatgtatgct gaatttgaaa aaactataca
    42301 cgagttagta gaaaaaggaa aaagtgtaaa cgcattagta ttaaatgatg tttggggaca
    42361 acttcttaaa aaatattatg gacaaagcta tgaattagac caattatcta aggtcggatg
    42421 ggctagaatc ccacattttt ataatagttt ctatgtctat aaatatgcta caggttgtag
    42481 tgcaggagta agttttgccc aagacatcct aaagaatggt tcagataact atatgagctt
    42541 tcttaaaaag ggaagttcag attatccaat aaacttgtta aaatcaagtg gcataaattt
    42601 aactaataca aaacccatag aaaatacaat caaaaaattt gatagtttag tagcagaact
    42661 tgaaaagcta ttataaacat tattgtaatc cacatggtaa atatatcgaa ttaatgataa
    42721 aatatactaa aagtttaaag tcctatcaag ataggacttt ttatttatta attagtggga
    42781 gatgattcta tgcaagttaa agttaaaaag gctgtaatac cagcagcagg tttaggaact
    42841 agatttttgc cagctacaaa ggcacaacca aaagagatgt taccaatagt tgataagcca
    42901 actctacaat acataataga agaagctgtg gcctcaggaa tagaggaaat actaataata
    42961 acaggtagaa ataaaaaatc tattgaagac cactttgata aatctgtaga attggaactt
    43021 gatttagaga aaaaaggtaa gaaagaattg ttggaggtag tacaaaacat atctaacatg
    43081 attaacatac attatataag acaaaaagag ccaaaaggat tgggagatgc aatttattgt
    43141 gcgaggcatt ttataggtga tgagccgttt gcagtaatgt taggggatga tattgtagat
    43201 aatgatgtac cttgtctaaa acaactaaca gatgcatatg aggaatatag aactacaata
    43261 ttgggtgttc aaaaggtaaa tcaagaagat actaataaat atgggataat agaagcaaaa
    43321 aatatagaag gtagagttta caaggtaaaa gacatggtag aaaagccaga gtcaggaaaa
    43381 gctccttcaa atattgctat tttaggtaga tatataataa ctcctgaaat ctttgatatt
    43441 cttaaggatt taccaccagg taaaggtgga gaggtacaac ttacagatgc tttaaaaata
    43501 ttatctaaga aagaagctat gtatgcatac aattttgaag gcaagagata tgatgttggg
    43561 gacaagctag gatttttaga agctactgtt gactttgctc taaagaaaga agaccttaag
    43621 gaagatttta taaaatattt gaaacatgta tgtagtgaat ttgataaaaa taacaatgtg
    43681 ttatacaata atacagatga aaaaatagat ttaattgaag tcaatgaagc tagagaactt
    43741 gaaattcaaa aataattaat gaaaggtgat gtatggtggc aaaatcaagt cagaaaagaa
    43801 ttaatagaat aatagctgtt gggattgtgg gaatcctcgt aataacgagt ataacagctg
    43861 ttttgagtat gattgcaact atgtaataag ataaatgaca gcagttgata tacataaata
    43921 taaaaaccat atataacttg atgtagtcaa aaacttacaa tattttagtt atatatggtt
    43981 tttagaattt ataaatgtaa aaaaaaattt ttataatagt tgcatatttt aaaggaaaat
    44041 attattataa gatataaata tagtgataaa tttataaaaa cgtgctaaga ttggacttga
    44101 aatacagtaa tatagtaggt aataatgttt taaattttat ttattgacca taatatataa
    44161 ataatgatat tattagactt aactgtttaa ataaaacttt tctttttcat gccttcaatt
    44221 tccaaaataa gtatgtatat aaatgaatat atagatgagg aggatttttt tgattttaga
    44281 tgataaagta ttagaaggat taggtagtaa gaccttagtt gagctacgag agatagctaa
    44341 agaattaaaa ataaaatcaa ttactacata taagaaaaat gagttaattg agattataaa
    44401 ttccaaaagt aaaaatgagg aaaaagtaaa cgaagagact aaagaaaaaa atgaagatat
    44461 taaagaagac ataaagaata gaaaagaaaa tatagagcgt gaaagtatag aaaaagaagt
    44521 tgaatataag agccctacca aatcatacaa caagaaagaa atagataccc aaaatatttc
    44581 ccaaaaccaa gaaaatagaa atcaaagaaa tgactataat aagacaacga gtgtaaatga
    44641 tagtaataag tcatcaaata tatctaatag aatggtcaga aataataata aaaactatta
    44701 tatgcctaaa caagttgatg aatctaagat agtagatgaa tttaacacat caaaagagga
    44761 tgaagtagta ggtgtacttg aaattctgcc agatggattt ggttttttaa gaggttcaaa
    44821 ttatttgtct actgaaggag atgtttatgt ttcaccatct caaataagaa gatttaatat
    44881 gaaaacagga gacaagatta agggaataac tagacatcca aaaagtggag agaaatttag
    44941 agcactttta tatgttcaaa aaataaatga tgaaaaccct gacacagcta tacaaagaaa
    45001 tgcgtttgag acattaacac caatatttcc agaagaacga cttacactgg aaactaatag
    45061 aaatgaaata gctacaagaa ttatagattt aatttcacct ataggaaagg gacaaagagg
    45121 attgatagta gcacctccaa aggctgggaa aacagttctt ttaaaaagcg ttgcaaatag
    45181 catagctaaa aatcatccaa atgttgaact tatagtactc cttatagatg aaagaccaga
    45241 ggaagttact gatatgaaag aatctattga aggagacgtt atatattcaa cttttgacca
    45301 agtatcaagt catcatgtca aggttgcaga gatggtgtta aatagagctc aaagattagt
    45361 tgaacatggt aaagatgtag taattttact agatagtata actagattgg caagggcata
    45421 taaccttaca atatctccta caggtagaac tttatctgga ggtattgacc ctggtgcact
    45481 tcatggacct aaaaagttct ttggtgcagc aaggaatatt agacagggtg gttctttgac
    45541 aatattagga acagcattgg ttgaaactgg ttctaggatg gatgatgtaa tatttgagga
    45601 atttaaaggt acaggaaata tggagctaca tttagataga aaattagcag aaaaaagaat
    45661 attccctgca atagatattt acaagtctgg tacaagaaga gatgatttat tgcttgatga
    45721 tgaagaaaag actgctcttt ggagattaag aagagaaatg agtaataatt cagtaatgga
    45781 aattacagat aaagtcattg aacttataaa gagaactaag gacaataaag agtttgtaaa
    45841 atcaataaag aatttgtaaa atcaataata caaatgcaaa tatggattat ataaactaat
    45901 agaaaaatca cttgaatggc ataataagtt gtgctataat aatgtagttg ataatgtaaa
    45961 aagaaagtta atataattaa tttttttaaa taaagagaag aggtgactat aatgcaaaag
    46021 gaaatacaac caaaatacaa tccagttgaa gtgcgttgtg cttgtgggaa cacatttgtt
    46081 gctggttcaa ctaaggacga aataaaagtt gaaatatgtt cagagtgcca tccattctat
    46141 acaggaaaac aaaagaacat agaaaaaggt ggaagaatcg acaaattcaa gaaaagattc
    46201 aaaatggact aatttttata aaagtggagt tggaggaagc caactcctat tttattacaa
    46261 aaaaacattc atattctact ttaaattgtc tttttgtatt ataataataa atatgtaaaa
    46321 ttgagtattc taaatacaat aaaatgaggt gagtggatgg caaaacaagc tgtaggtggg
    46381 caagctgtta ttgaaggtgt aatgatgcag tcaaaagaca aaagagcggt agctgttaga
    46441 aaaagtgacg gagaaatagc actaaaagaa gatagaataa aaagttgggt aagagataag
    46501 aatatagata agataccttt tgttagaggt tcttttgtta tgatagatac aatgattcaa
    46561 gggataaaaa gtttaaactt ttcttcagag ttttttatgg aagaagctga agaggataaa
    46621 tttgatttat ttataaagaa aatttttaaa gataaggcaa atgatataat tataatattt
    46681 tcattggtca tagcaatgtt actttctgct ggattattca tttttatacc tacattggta
    46741 ggaggagctt tttctaaagt aatgcctaat gattttatgc ttaatttaat agaaggaatc
    46801 ataaggatag ctatattatt tgcatatata gtattgattt cacgaagtaa ggatatagaa
    46861 agagtctttc aatatcatgg agcagaacat aaatctatat attgctatga aaatgattta
    46921 gaacttacag tagagaatgc tagaaaattt aaaagattac atcctagatg tggtacaaat
    46981 ttcctattta tagttatggc tgtatcaata atattgtttg cattttttgg ttggccaaat
    47041 ccaattctta gaatatttat gagaattata tgtgtgccta tagtagcagg tttatcttat
    47101 gaggtaatta gagttcttgg gaaatatgat aatggattta ctaaaattat tgcatatcca
    47161 ggaatgatgt tgcaatattt tacaacaaaa gagcctgatg atgagcaatt agaggttgct
    47221 ttggaagcat tgaaagcagt agtagattag agaaaaggat atttgttatg acgataaaag
    47281 atataattat taaatattca gataaactaa aagatataag tgatactcca agattagata
    47341 cagagttatt gttacaaaaa acattgggtg ttgatagatt atatattcac ttaaatctga
    47401 ataaagagct aactgaagaa caaaaaacaa aatttatggg ttttgcagaa gaaagattaa
    47461 atggaagacc aatagcatat atagttgaaa atagggagtt tatgggatta gatttttttg
    47521 ttaaggaagg cgtattgatt ccaagaccag atacagaaac tttagtagaa gaaataattg
    47581 aaatatgtag agaaaaaaaa gatgtaagta tattagatat aggaactggt tctggagcta
    47641 ttacaataag tttagcaaag tacattgaaa attctaaaat catgtctttt gatatatcag
    47701 agacagcatt agaaatagct aaaaaaaatg ctattataaa tgaagttggt gaaaaaataa
    47761 agtatattaa ttctgattta tttacagcaa taagtgatag taacataaaa ttcgatatca
    47821 tagtttcaaa tccaccatat attaaaaaac aagatataga aactctgcac aaacaagtta
    47881 aagattatga accatataat gctcttgaag gtggagaaga tggattagac ttttatagaa
    47941 gaataacaga acaaggtaag aaatatttaa ataagtgtgg aatattagca tatgaagttg
    48001 gtcataatca agctgaagat gtaataaata ttatgaaaag taatggatat aaaaaaatat
    48061 acacaaagaa ggatattcaa ggcattgata gagttgttat aggttacaat atatgatata
    48121 atcaacaatt gatatgttac atttaatcga ggtgaaagta tgttaaaaaa attagaggta
    48181 ttggaagata catataaaga cttaagtgaa aaaataggtg atccggatgt aataaatgat
    48241 caaaaagttt ggcagaaata tataaaagaa catgctgact tggaacctat tgttatgaaa
    48301 tacagagagt ataaaagcgt tttagatagt ataaaagaat caaaagaaat tttacaagaa
    48361 gagtcagatg aagaattaag agaattagct aaaatggagt tagctgagat ggaagaaaaa
    48421 gtagcacctc tggaggaaga aataaaaata ctattattgc ctaaagaccc taatgatgat
    48481 aagaacgtta tagttgaaat tagaggtgga gctggaggag atgaagcagc tctatttgcg
    48541 ggtgatttat ttagaatgta ctcaaggtat gctgaaagaa gaagatggaa aatagaatta
    48601 ttgagtgcaa gtgatactgg agttggtgga tataaagaag tatcttttat gataaaaggt
    48661 aaaggtgctt attcaagatt aaagtatgaa tctggagttc atagagttca aagaatacca
    48721 tcaactgaat ctggtggaag aatacacaca tcaacttcta ctgttgcagt attaccagaa
    48781 gttgaagatg tagaggttga aataaatcca aatgatttaa gaatagatgt tttccgttct
    48841 tcgggtaatg gaggacaaag tgtcaatact actgactctg ctgttagggt aacacatata
    48901 cctacaggtg aagtagtatc ttgtcaagat ggaaaatcac aattaaaaaa taaagagcaa
    48961 gctttaaaaa tattaaaagc tagactttac gataaagcat tggcagaaca acacaaagat
    49021 atagcagcag aaagaaaaag ccaagtcgga acaggagaca ggtcagaaag aataagaaca
    49081 tataatttcc ctcaaggtag aataagtgac catagaataa atttaacttt atataagtta
    49141 gatgcatttt tagatggaga tatagatgaa atgatagatg ctttaattac tgttgaccaa
    49201 acagaaaaaa tgactgcaat ataagtaatg ttttaaagaa gctgtttctt cctagaaatt
    49261 attttctatt ttgaaacagc tattttgcat tttaggtcat gttatcatat tctattgtat
    49321 ataattgtta tatacaagta taggagggtt atatgatact aaaagtaaca ataattgggc
    49381 ttttggcagg tgttataggg acaggattag gaggagtaat atcagccatt tttaaaagag
    49441 aggtcgataa atatctaagt ttttttatgg gattatctgg tggaataatg ttggctgtag
    49501 tggtgtttga tttaatgaaa gaatccatgg acaaaatggg gataatcaat acagttatat
    49561 ttacgtttgt tggagctctc ataactatgt acataaaaac aaaattagat gtgtctggaa
    49621 atatggcatc tggatatctt atattcataa gtatattact acataattta ccagaaggac
    49681 tagccattgg ttcttctttt atgtcaactg aaagtttagg tattacactt gctatagtta
    49741 taggtctaca taatatacca gaagggttag ctatggcatt aggcttagtt tgcaataaaa
    49801 tgaaactaag taaagttatt ttgtttactg taatagctgg attacctatg ggtctaggaa
    49861 gttttttagg tgtttatttt ggaggagtat ttacttctct tataggagta tttcttgcta
    49921 cagcaggagg tactatgatg tatgttgtat tggaagaaat ttttccacat tcaaaaagtg
    49981 tgtattgtat aattggtttc ttgttaggta caatgattgt aaattatata taaaattaaa
    50041 atgaaagcag gcatacaaaa gatgaaaact attataagta atattgatat aaataatatt
    50101 gattatgagg aaataaaaat acaagcaaaa cttcttagag aaggtaaaac agttattttt
    50161 ccaactgaga cagtatatgg gttgggtgca aatgcattag atgaaaatgc agtaaagaag
    50221 atttatgaag ctaaaggaag acctagtgac aatccattga tagtccatat atatgaaaaa
    50281 gaagaagtat atgacttagc caaagatata agtgataaag ctaaacttgt tatagaaaag
    50341 ctttggccag gacctataac aattatatta aataaaaaag atataattcc atacaaaact
    50401 agtggagggc ttgatacagt tgcaataagg atgccttcta atgttattgc cagggcaata
    50461 ataaaagaag ctggtatacc tattgcagct ccttctgcaa atatctcagg tcgcccatct
    50521 ccaacaaaag caaaacatgt atatgaagag atgaatggaa gagtggatgg aatagtttta
    50581 ggaggagaca gtaattttgg attggagtca actgtattgg atttaacaga agagactcct
    50641 atgatactga gaccaggaag tataactaaa gaagttttag aggaacttct tggagaagta
    50701 aagcttgacc catcattaag taaaaaagaa gataatcaaa aagctaaagc tcctggaatg
    50761 aaatataaac attattctcc aaatgcagat gtatatataa tatctggaaa aagagaaaat
    50821 gtagcaacaa agattaatga tatgataaaa tctaatagag acaaaggtct aaagacaggt
    50881 gtaatgtgta tcagtagaaa ccgagagttt tatgatggag aagtaatcga tttaggaaac
    50941 tctcttgaag aagttgcaag taatctgttt gatgctctta tagaaatgga caaaaagggc
    51001 gttgatgtca tatattcaga agagttccct aagactggag ttggacaagc aattatgaat
    51061 agactactga agtcagcagg atacaaagtg ataaaggctt aagagggagt tgtaaaatat
    51121 gaatatactt attgtgtgta caggtaatac ttgtagaagc cctatggctg aggctatact
    51181 aaggaaagca attaaagaat ctggaagaag tattgaagaa tattcgatat catcagcagg
    51241 tatatctaca gcaaatggaa tgggtgcttc tgaaaattct atagaagtac taaaagaaat
    51301 cggtatagac ttatcaaatc atagaagtaa agtaattact aaaaaattaa ttgatgaatc
    51361 agatataata ctaacaatga ctaaatctca taaagaaatt ttagtacaag cagttccaaa
    51421 atgcaaagaa aaagtgtata cttttaaagg gtttgcaaat aaaaatgagg aagatatatc
    51481 tgacccattt ggtggaaatt tggatattta tagaagcact atgagagaaa taatgtattc
    51541 tgtcaacgaa atagtaaaaa aaatatgaaa cggaggataa acaaatgaag ataggattag
    51601 gctgtgacca tggaggatat aatttaaaaa aagaaataat aagctattta gaaagtaaag
    51661 gtatagaatg tgtagattat ggaactaata atgctacaga ttctgttgat tatcctgttt
    51721 atggagaaat agttgcaaat tcagttataa ataaagaagt agattatgga atattatgtt
    51781 gtggtacagg gataggaata tctttagctg ctaataaggt tcctgggata agatgtgcag
    51841 ttgtatcaga tgtattttct gcaaaaatgt caaaagcaca taatgatgca aatatgttat
    51901 cattaggcga aagagtttta ggaaaaggtc tagcacttga gatagttgaa gcatggatta
    51961 atacggattt tgaaggtgat agacatgcta gaagagtaaa catgattaaa tcaatagaag
    52021 aaaaacataa taaataataa aaattacagt tttatcagga ggaattatca aaatgagcaa
    52081 agtggtagag acaaaccatc cattaataca acataaatta actttaatga gagataaaaa
    52141 tactggttct aaagacttta gagagctttt aactgaaatt gcaatgttaa tggggtatga
    52201 aataacaaaa gatataccat taaaagatgt agaaatagaa actcctattc aaaaaacatc
    52261 atctaaagta gttgcaggta aaaaattagc aataattcca attttaagag caggtcttgg
    52321 aatggtagat ggattagtaa gcttaatgcc agcagcaaaa gttggacatg taggactata
    52381 tagagaccca gaaacattaa aaccagttga atattattgc aagcttcctc aagacatagg
    52441 agaaagagat ataatagtag ttgaccctat gcttgcaaca gggggttctg ctgtagcagc
    52501 tatagattta cttaagtcta aaggagctaa aagtataaaa ttagctaatt tagtagctgc
    52561 accagaaggt atagcagaag tgcaaaaata tcatgatgat gttgatatat atgttgcatc
    52621 agttgacgaa agattaaatg aacatggata tataattcct ggtcttggtg atgctggaga
    52681 tagattgttt ggaactaaat aaaaataaat aactataaat atttattagg gactttctta
    52741 tatagtgtat ataattttat gtaagaaggt tcttttttat atattttttc tcaaaatagg
    52801 aattatgaat tgataaattt ctcgaattga gaataaaaag tttttaccaa ttaaaaaaga
    52861 ttgcttattt tacaaggttt tatagaatag tagtctattt taatgtggca tgttagttgc
    52921 ttacgattaa gattataggt attaagtgta ttagttaaat tgagaattga atattaataa
    52981 tattattggg agtggtaata catgaagaat tgtatagcag taagaggggc tggagattta
    53041 gcaacagcag ttattcataa attacacaac agtggtttta aagtagttgc gttagaaata
    53101 gaagagccat tagctataag aaggcaaatt tctttttgtg aggctgttta tgaaaaaaaa
    53161 gtaactgttg aaggtgtaga atgtatattt tgcaatactg aaaaagaaat agaagaagtt
    53221 ttatctcaac ataaagtagc cttgattata gacccaaaag gagaaaaatt atctaagtta
    53281 aatccaatcg ttttggttga tggaatactc gctaagaaaa acttaggtac aactaaagat
    53341 atggcaccac ttacaatagc tttaggtcct ggattttgtg caggagaaga tgtagatgtt
    53401 gttatagaga caatgagagg tcataatcta ggaagaataa taactaatgg tagagcagaa
    53461 aaaaatacag gagtaccagg agcaataaaa ggtttttcta aagagagagt tatgcattca
    53521 cagtatgatg gtataatgga aaatgtatgt aagataggtg atatagttga aaagggagac
    53581 ctactttgtt atattaataa ttatgaaaaa aaacatgaag ttagagcaac aatatctgga
    53641 gtccttagag gtttattaaa ggatggagca aaggtgaaca aaaatttaaa aatactagac
    53701 atagacccta gaaaagaaga agttaagaat gcttttacaa tatcagataa agctagatgt
    53761 atagctggag gggttttaga agccatacta aacaatggga tttatcccca aaattaattt
    53821 atattagata aagctattaa tattttatta agatgatata ataaatttaa tagattaaag
    53881 agagcatatg acgggtattt aaaatttaaa tggtaaaaat gctctttttt tattggacat
    53941 tcaattaaca tgtgtatata atataaaaat aagtatttat ttaggttgaa atctaataaa
    54001 tatataatac cagtacataa gcaaagagga gaaaagtaaa ttatgagacc atcatgggat
    54061 gaatacttca tggaaattgc agaagtagtg aaaaagcgtt caacttgtat tagaagacaa
    54121 gtaggagctg taattgtaag agacaaacaa atattaacaa ctggatataa tggttctccg
    54181 agaaatcttg aacactgtga aaatattgga tgtaagaggc aggaattaaa tattccctca
    54241 ggagaaaggc atgaattatg cagagcatta catgctgaac aaaatgcgat aatacaagct
    54301 gctcataatg gtattagtgt agatggggca actttgtatg ttactacaag accttgtgtt
    54361 ttgtgtgcaa aaatgtgcat aaatgcaggt atattgaaaa ttgtttatga aggtgattat
    54421 ccagatgata tgtctacaga attattaaaa gaagcaggaa tagaactcat aaaattttag
    54481 tggataaaat ttatatgaca ataaatgtca aattatgaca ctgaaataca atggtatcaa
    54541 atgattaaaa gaaagtattt gaaaaatcat cagattagta gtaagatata ttttataatt
    54601 aaataaattt atgagataga ttaattacat gttatttatt ttataataat gagggatact
    54661 atttatgagt aaaagaaata cacatacaga aattgcaaag ttgtttagtt taataagtca
    54721 aataggattg atgattgtaa tatcaatatt aggatgtact ttcttaggaa aatttttaga
    54781 tagtaaattg aatactactc cagttctgac aataatattt ttattgttgg gtgttggagg
    54841 tgcttttagt ggtgtttata aaactctaat agtatataca cgaaggaagt gatatattgg
    54901 atataaaatt acttaaccag gtaaggtcag taacaaaagg tatagtaata tttgatgtag
    54961 ttttaattgt gctaatgttg ataacatcaa cattaagtaa accagtatta ttaggactta
    55021 tatttggctc tataatagct atgttaaatt ttagattatt ggcgatatca ctggaaaaat
    55081 cagtgacatt tcctgctggg aaggcacaag cttatgcatc agcacaatat gctattagat
    55141 tatttataat ggcagttgtt ctaattgcat cagctacagt accacattta aatataatcg
    55201 gaacagcatt gggattattg agcacaaagt ttgtcatatt atcaaaaaac tttataaata
    55261 aattaaagag gaaggaggca taagtatgaa tcaagcgcat tattatctca taggaaactt
    55321 taagattagt gaaacaatac tagtaagttg gggaataatg gcctttttag cgatagcatc
    55381 ttattttttg actagaaact taaaaaaagt tccaactagt aaagttcaaa tattcttaga
    55441 atttgcagta ggtggtcttg ctaaaatggt tacagatgtt atgggtaaaa atacagtaaa
    55501 aagaatgcca tatatgatac catacatagg aagcttgttt ttatttttcg catgttcaaa
    55561 tttaagccca ttacttggat taaggtcacc aacaaccgac ttagacacaa ctgctgcatg
    55621 ggcaatgatt acatttttta tgatttattt tgcaggagtt aaatttaagg ggttatcata
    55681 ctttaaagaa ttggctcaac cacttgccat aatgttacca atcaatatta taggtgagtt
    55741 agctagacca atatcattaa gtttccgtcc atttgggaat attttaggtg gaacaataat
    55801 aatgggtctg ttttatcaac ttttaggttt tatatctagt ttaataccag gattatcaat
    55861 accaattgga gaaatattaa taccagtccc attacacttg tactttgact tatttgcagg
    55921 attgttacaa gcatatatat ttataatgtt aacaatggta tttatatcaa gtgcaggaga
    55981 gttagattaa gaaatcaact tatattaaga taaaaataaa ataatttaaa aaattaaagt
    56041 tttaaggagg aaataattat ggaaacagca atagtagcgg cagcatcagc aataggagca
    56101 gggatagcag ttgcaacagg tataggtgca ggtataggtc aaggtatagc agcagctaaa
    56161 gctgcagaag cagtaggaaa ccaaccagaa gctaaaggtg atataacatc aactttatta
    56221 ctaggagttg ctatagcaga gtcttcagca atatatggtt tggttatatc aataatactt
    56281 ctattcgtaa atccattctt taaatactta ggaatgtaga tttagtcctt aaatattttg
    56341 agataggttt ataggtaaat catctcaaac ttataatcat gagatgaact ttatcatctc
    56401 gtgattataa gtagttatga tttatggaaa ggaggataat tatggaaaaa gcactagtag
    56461 gtataacgtg ggaatttgtg ttccaaattg ttaatacttt tataatattt ttacttctga
    56521 gaaagctttt attcaagcca gtattaaata taattgaatc aagagaaaat gatattaaaa
    56581 gtgatttggc agaaggcgaa aaagcaaaaa atgaaggttt agctctgaaa aaagaatatg
    56641 agtcaaaaat caattttgcc aaggatgaag gtcaagaaat cataaaacaa gcaactataa
    56701 gagcagagca gaaatcagat gatatagtaa atactgcaaa gaaagatgct cttgatataa
    56761 aagaaaaagc aaacaaagat atagaacaag aaagacaaaa ggttataaat gaaatcaaaa
    56821 atgatatatc taacatagca ctacttgcag catctaaagt tattgaaaaa gatttagata
    56881 aatctaaaca tgaagaattg atagaaaact ttataaaaga ggtaggggaa gctaaatgat
    56941 aaatgtaata gcaaatagat atgccgaagc cctatttcaa ttaggagaag aagaaaattc
    57001 aactgatgtc ttattcaaag aactagaaaa agtagtagat atgatgacta aagttagtaa
    57061 agatttctac aaagttttaa aatcaccatt agtttcaaaa tcagaaaaga aaaacttggt
    57121 agaaattatt tttagtaagg aagtaagttc taacataaaa aactttttaa aagttttagt
    57181 agataaagat agaatatcat atttagaaga tatcgaatta gcttataaag aattattaaa
    57241 taagaaaaac aatgtaattg atggagtagc aataagtgct attccaatga gtgaaacaga
    57301 tattaaagaa cttgaagtca aactttcaaa taaatacaac aaaaatgtta ctatagaaaa
    57361 tgttgttgac aagactatac tagggggagt tctagttaga attggaaatg aacaaataga
    57421 tggcactgtg aaaactcgtc ttgataagat gaaagaaaag ctatctgaag taatatctta
    57481 agaatggcgg tgaacccatg aacttaaaac ctgaagaaat aagttctata attaaacagc
    57541 aaataaaaaa ttatgagaat aaagttgagt tgacagatac aggtagtgtt ttaacagttg
    57601 gggatggtat agcgagtgta tatggattag aaaaagctat gtctggtgag ttgctagagt
    57661 tcccaggtga aatatatggt atggcactta acttggaaga agaagtagtt ggagctgtta
    57721 tccttggaga tgacagtgaa ataaaagaag gcgatatagt taagagaact ggaagaatag
    57781 ttgaagttcc agttggagaa gctttaatag gtagagttgt aaactcacta ggtcagccaa
    57841 tagatggaaa aggacctata gcgtacacaa aaactagacc agttgaatct gaggcacctg
    57901 gtataataga tagaaggtct gtttatgaac cattacaaac aggaatcaaa tcaatagatt
    57961 caatgatacc aattggtaga ggtcaaagag agttaataat aggtgacaga caaactggta
    58021 aaacatctat agttattgac actatactaa atcaaaaagg aaaagatgtt atctgtatat
    58081 atgtagcaat tggacaaaaa cgttctacta tagctcaatt agttagctct ttagaaaaag
    58141 gtggagcttt ggattataca atagtagttt cagcaacagc atctgaatca gcaccacttc
    58201 aatatatagc accatatgct ggagcagcta tgggtgaaga atttatgtac aatgggaaac
    58261 atgtacttat agtgtatgat gatttaagta aacaagctgt tgcatataga gaaatgtcat
    58321 tgttacttag aagaccacca ggacgtgagg catatcctgg agatgtattc tatttacatt
    58381 caagattact tgaaagagca gctaagttat ctgatgaatt gggtggaggt tcaatgactg
    58441 ccttgcctat aatagaaact caagctggtg acgtttctgc atatatacca acaaatgtta
    58501 tatctataac agatggtcaa atatacttac aaccagagtt attctattca ggtgtaagac
    58561 cagcagttga ccctggtata tcagtatcaa gggttggtgg ttctgcgcaa attaaagcaa
    58621 tgaaaaaagt tgcaggaaca ttaaaacttg catattcaca atatagagaa cttgcagcat
    58681 tctcacagtt tggttctgac ttagatgaag atactaaaaa gagacttgct caaggtgaaa
    58741 gaatcgttga gatattaaaa caaggtgagc atcaaccaat aaaagttgaa aatcaagtta
    58801 tgataatata tgctgttata aataatcatt tagaggatat tccgatagat aatatagcta
    58861 gatttgaatc agaattgtat gcatttgtag ataataatta tccggaaata tcaagaaaaa
    58921 tattaggtgg agaagacttc actcatgatt taacagatgc aataaatgaa tttaaagaaa
    58981 agtttgttgt tgaagtataa tcctttttaa aaaaggaggt aaattaattg gcaggagctg
    59041 gaatcaaagc aatcaaaaca cgtatgggaa ctgttgaaag tacaaagcaa ataactaaag
    59101 caatggaatt agttgcatct tctaagctta gaaaagcaaa agaaaaggct gaagattcaa
    59161 gagaatattt taatacttta tacaatacag taaaagatat tgcccaaaca acacgtggaa
    59221 ttagaaatga atttttaaaa gaaagagaag taaaaaataa atgttatgta gtagtagctg
    59281 gagacagagg acttgcaggt ggatacaatt ccaatgtatt aaaggaatct gtctctcata
    59341 tgaaaggtaa aaaggaaaca gtgattactg ttggaaagaa agcacatgaa tttttttcta
    59401 aaagaaacta caatatagca aaagatataa atagtgttga agcttgtgga tatgaagatg
    59461 cgacagaaat ttcacaaact attattgacc tgtataaaaa aggtgaagta gatgaagttt
    59521 atattgctta tacaaaattt aaatctgctt tatcacaaga agttgaaatt ttgaaattgc
    59581 ttccattagt ctttgaaaaa gatacagacg aacaaggaat ggcagtaaaa gaagaaaaac
    59641 aaaatagatc aaaagtacaa tatataccat cagcagaagc agttttaagt tatattgtac
    59701 ctcagtttat atcaggtaca gtatatggtg gaattttgga atcttttgca tcagagcaag
    59761 gtgctagaag aactgcaatg gaatcagcca cagacaatgc gaatgaaatg ctatctaatt
    59821 tagagttaag ttataacaga gcaagacagt cagctgtaac tcaagaaata acagagatag
    59881 ttggtggagt tgaagctcta aaatagggag gttaggaaat ggcaaatgta ggtaaagtag
    59941 tacagattgt aggagctgta ctagatgtta agtttgatag tgaacaaagc ctacctaact
    60001 tattaaatgc gttagtaata aagttaggag ataaagaaat agttgcagaa gttgctcaac
    60061 atataggtga tgatactgta agatgtatag ctatgagtgc aacggacgga cttgtaagag
    60121 gtatggaagt tgtagataca ggagggccaa taagtgtccc tgttggagat gagactttag
    60181 gtagaatatt caatgttctt ggaaaaccag ttgatgggaa accagctcca aaatctgcac
    60241 ctaaattacc tatacataga ccagcaccgg cgtatgatga actagaaact acagcagaga
    60301 tactagaaac tggtattaag gtagttgact tactagctcc atatctaaaa ggtggaaaaa
    60361 taggtctatt tggaggagca ggagtaggaa agacagttct aatacaagag cttataaaca
    60421 atattgctaa gcaacatggt ggtatttctg tattttcagg agtaggagaa agaacaagag
    60481 aaggtaacga cctttatggc gaaatgagtg agtctggagt tataaataaa acagctctag
    60541 tatttggtca aatgaatgaa ccacctggag cgagaatgag agttgcttta actggactta
    60601 caatggcaga acattttaga gatgagcaag gacaagacgt tttacttttc gttgataata
    60661 tattccgttt cacacaagct ggttcagaag tttcagcact tctaggacgt atgccatcag
    60721 ctgttggtta tcagccaaca ttagctactg aaatgggtgc acttcaagag agaataacat
    60781 caactaagaa aggttcaata acatctgttc aagcagtata tgtaccagca gatgacttaa
    60841 ctgacccagc accagctaca acattctctc acttagatgc aaaaacagtt ttatcaagac
    60901 aaatatcatc actgggtata tatccagcag ttgaccctct tgaatctaca tcaaggattc
    60961 ttgaccctag tatagtagga aaagaacatt atgaagtagc tagaggtgta caatcaatac
    61021 ttcaaagata taaagaactt caagatataa ttgctatact tggtatggat gaattatctg
    61081 atgaagataa gttaatagtt gcacgtgcta gaaagataca aagattctta tctcaatcat
    61141 tcacagttgc tgagcaattt acaggaaatc caggtcaata tgttcctgtt aaagagactg
    61201 taagaggatt taaagaaatt cttgaaggga aacatgatga cttaccagag tcagcattct
    61261 tatttgtagg aacaatagaa gacgctgtta gaaaagcgaa gggaagtatg tagtcatggc
    61321 cagtgaattt tcattgaaag ttgtaactcc aagtaatact ttttatgatg gagatgttga
    61381 aatgatagta gttagaacta caaaaggaga caaaggggtg ttgaaaggtc atataccatt
    61441 tgtttcaggt atacctgtag gaactttaaa gataaaaaaa gagggaagtt ttaaagaggc
    61501 gaaaatagca gaaggattca ttaatgtaac tcaagataaa actgtcatat tgacagaatc
    61561 tgctgagtgg atttaataaa ttaatttaga gtataaaaag tcattttgac caaaaaaact
    61621 tttgggtaaa atggcttttt tgtgttttgg tgttaataaa ataacgataa aattgtaagt
    61681 ttttagtgat aataaattca caaaatgttt aaatttactg ccaaattatg ttgtaattaa
    61741 taagataaag aagatttaga tagcttttat gtaagtgaaa aagtcttcat ataagaaata
    61801 catataaaat ttttataaaa tttaaaaaat ttgcttagtt gtgta
//
LOCUS       DAMJVX010000006        60003 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMJVX010000006 DAMJVX010000000
VERSION     DAMJVX010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 60003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60003
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.006"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            63..629
                     /locus_tag="PC407_000317"
     CDS             63..629
                     /locus_tag="PC407_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2495799.1"
                     /translation="MDTKKVTFSSICIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            complement(846..2909)
                     /locus_tag="PC407_000318"
     CDS             complement(846..2909)
                     /locus_tag="PC407_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDF2495800.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFND
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDVYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(3290..6625)
                     /locus_tag="PC407_000319"
     CDS             complement(3290..6625)
                     /locus_tag="PC407_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2495801.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEKLRKSLTEKRKVNTVNYDFKIDEVNEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGKYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMVTAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRKLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(6675..7103)
                     /locus_tag="PC407_000320"
     CDS             complement(6675..7103)
                     /locus_tag="PC407_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495802.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(7093..7473)
                     /locus_tag="PC407_000321"
     CDS             complement(7093..7473)
                     /locus_tag="PC407_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDF2495803.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(7506..7814)
                     /locus_tag="PC407_000322"
     CDS             complement(7506..7814)
                     /locus_tag="PC407_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495804.1"
                     /translation="MNQIELDLYHDMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(7847..9031)
                     /locus_tag="PC407_000323"
     CDS             complement(7847..9031)
                     /locus_tag="PC407_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDF2495805.1"
                     /translation="MVDEAKILDVVSISRENVKKFDGERSYLSTGNLDFNKISNLEIV
                     TYENKPSRANQTVNIGEVIFAKMKDTKKTLVINKTNKNIIVSTGFYVLKPSKEILPQY
                     LYHYLNSSYFLNQKNRLSKGATQSALNNEGLANIKIRMYNLKVQEKVVRVLDKAQELI
                     DKRKEQIEVLDELVKSRFIEMFGTPSKNEKNWEISEIGKYLDVLTDYHSNGSYETLRD
                     NVTLLDTKGYALMVRTTDLENNNFEKGVKYIDEHAYNYLEKSKVFGGEVIINKIGSAG
                     KVYLMPFLNKPVSLAMNQFMLRFNEDKVNHIFLYNLLLTSYMESKIKEKVRGAVTKTI
                     TKDAVRKINIIVPPIRLQNQFANFVKQVNSLKFEMETSLKELEDNFNSLMQKAFKGEL
                     FN"
     gene            complement(9035..10498)
                     /locus_tag="PC407_000324"
     CDS             complement(9035..10498)
                     /locus_tag="PC407_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDF2495806.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFEKIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KESAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVKVLEKEIVDGLEELERMLEV"
     gene            complement(11008..11877)
                     /locus_tag="PC407_000325"
     CDS             complement(11008..11877)
                     /locus_tag="PC407_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2495807.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIGTVKC"
     gene            complement(11897..13813)
                     /locus_tag="PC407_000326"
     CDS             complement(11897..13813)
                     /locus_tag="PC407_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDF2495808.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDGHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMISATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(13816..14736)
                     /locus_tag="PC407_000327"
     CDS             complement(13816..14736)
                     /locus_tag="PC407_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HDF2495809.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(14838..15590)
                     /locus_tag="PC407_000328"
     CDS             complement(14838..15590)
                     /locus_tag="PC407_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2495810.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(15887..16504)
                     /locus_tag="PC407_000329"
     CDS             complement(15887..16504)
                     /locus_tag="PC407_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495811.1"
                     /translation="MNVIKKFIFVILIINILGCGISLILQSSLGSDAITLLNEGIHIK
                     LGISYTIAGLIYNGTLLLIALLLNKKSLGLGSCVYVLVAGIFIDLYIYLLLDFNISSL
                     GIIIRIMSFIIGHILMCSGFAMLIKFDIGMSPLDAILIYIEGKFKYPYKVLKTIADLI
                     FLILGVYLGGNLGFGTIFSILCTGTTISIVSKMISVNKKRYYSSI"
     gene            complement(16592..18049)
                     /locus_tag="PC407_000330"
     CDS             complement(16592..18049)
                     /locus_tag="PC407_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2495812.1"
                     /translation="MIKLSKFPKDFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKHYESDMTVDKIKFAMKDNNGYYPKRNGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKDKVKYWIVINQINLMYHESFNSVAFCVDQVDDVEEAKYQ
                     AIHHQFMATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRIQYFFT
                     DVSFRGEYPAYIKRFFKENNINIVMEEGDDKVLKENTMDFLALSYYYSTTVSASKNTM
                     NPIDNVKNQYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYKNVIRTNGEELKEKVDSF"
     gene            complement(18071..18424)
                     /locus_tag="PC407_000331"
     CDS             complement(18071..18424)
                     /locus_tag="PC407_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2495813.1"
                     /translation="MTTEEHVIDEELVEVAMQIILRAGEARTEIKHALNDLERFDYKN
                     ADLKLAKAKEFMTEAHRAQTNIIQGEASGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(18453..19700)
                     /locus_tag="PC407_000332"
     CDS             complement(18453..19700)
                     /locus_tag="PC407_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2495814.1"
                     /translation="MGRLFMNKFIDLLSEKFTPVVNKITKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVALVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDSMLPIAVIMFIGWGLIYGLNFDMFEIIVRIFKPVSDIAQTLPGFV
                     LLNFLVVFLYSLGISGWVLSPITYAIQLSAIAANAAAVASGGLATNIYTYEVVQTGWI
                     TLGGWGATLPLVLMMCLSRAGRIKAIGRACIVPSIMNINEPVMFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYLPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEELKLESQEA"
     gene            complement(19708..20025)
                     /locus_tag="PC407_000333"
     CDS             complement(19708..20025)
                     /locus_tag="PC407_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2495815.1"
                     /translation="MKNIKVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLPEIDCLLLGPHLDYMKDEIQKKADEYNVPVACISKTAYGRLDGVKALEEALELI
                     LNK"
     gene            20273..22186
                     /locus_tag="PC407_000334"
     CDS             20273..22186
                     /locus_tag="PC407_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2495816.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     STGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNLTLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYFIDLSDDEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQILENIEIDEDEISYISLHVGSFFDRQKLLED
                     KILCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIS
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIKFMGNILYKNKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(22309..23463)
                     /locus_tag="PC407_000335"
     CDS             complement(22309..23463)
                     /locus_tag="PC407_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDF2495817.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(23745..26360)
                     /gene="mngB"
                     /locus_tag="PC407_000336"
     CDS             complement(23745..26360)
                     /gene="mngB"
                     /locus_tag="PC407_000336"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF2495818.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNKDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YNRNEIVNAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKISDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIINVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGKKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKIPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANVVFNKNIKTAKWTNLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(26504..28429)
                     /gene="mngA"
                     /locus_tag="PC407_000337"
     CDS             complement(26504..28429)
                     /gene="mngA"
                     /locus_tag="PC407_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDF2495819.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDSNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIVLAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKKADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVIGKPVAA
                     LNNGLINWLNGMTGINAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIHVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(28559..29284)
                     /locus_tag="PC407_000338"
     CDS             complement(28559..29284)
                     /locus_tag="PC407_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2495820.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(29547..30326)
                     /locus_tag="PC407_000339"
     CDS             complement(29547..30326)
                     /locus_tag="PC407_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HDF2495821.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(30319..31746)
                     /locus_tag="PC407_000340"
     CDS             complement(30319..31746)
                     /locus_tag="PC407_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2495822.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAVSAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGHEVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            32137..32859
                     /locus_tag="PC407_000341"
     CDS             32137..32859
                     /locus_tag="PC407_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2495823.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECIGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            33056..34723
                     /locus_tag="PC407_000342"
     CDS             33056..34723
                     /locus_tag="PC407_000342"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDF2495824.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNLENEKVREECYKVLEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(34819..36138)
                     /locus_tag="PC407_000343"
     CDS             complement(34819..36138)
                     /locus_tag="PC407_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2495825.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYADKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(36179..36925)
                     /locus_tag="PC407_000344"
     CDS             complement(36179..36925)
                     /locus_tag="PC407_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2495826.1"
                     /translation="MKKLVKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(37169..39178)
                     /locus_tag="PC407_000345"
     CDS             complement(37169..39178)
                     /locus_tag="PC407_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2495827.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEEIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQSFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQGVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYSITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(39616..41049)
                     /locus_tag="PC407_000346"
     CDS             complement(39616..41049)
                     /locus_tag="PC407_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2495828.1"
                     /translation="MVLRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSREVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMTKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(41136..43052)
                     /locus_tag="PC407_000347"
     CDS             complement(41136..43052)
                     /locus_tag="PC407_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2495829.1"
                     /translation="MRRYEQLSKEIIENVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASIGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVIIIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGVLTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTSVVAAMYFKLKDKKIKELCIPAVISGICGVIEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDSIVEISKDNNGQGVVEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTIMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKHFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIIENTQ
                     KHVNNSQELITVLA"
     gene            complement(43126..43986)
                     /locus_tag="PC407_000348"
     CDS             complement(43126..43986)
                     /locus_tag="PC407_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2495830.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKCIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(44337..45524)
                     /locus_tag="PC407_000349"
     CDS             complement(44337..45524)
                     /locus_tag="PC407_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2495831.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGDNKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIESGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(45538..46929)
                     /locus_tag="PC407_000350"
     CDS             complement(45538..46929)
                     /locus_tag="PC407_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDF2495832.1"
                     /translation="MSSLKKKKFTMPQTLTIIFSLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMVFLTIGLYLGF"
     gene            complement(47524..49224)
                     /locus_tag="PC407_000351"
     CDS             complement(47524..49224)
                     /locus_tag="PC407_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2495833.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLASNGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(49196..50599)
                     /locus_tag="PC407_000352"
     CDS             complement(49196..50599)
                     /locus_tag="PC407_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2495834.1"
                     /translation="MIVNKELIIDYLKSNQENIIKDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGENGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTDDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSSAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(50863..51354)
                     /locus_tag="PC407_000353"
     CDS             complement(50863..51354)
                     /locus_tag="PC407_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2495835.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPNEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(51380..52192)
                     /locus_tag="PC407_000354"
     CDS             complement(51380..52192)
                     /locus_tag="PC407_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2495836.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLMGYEYKKVLSKEAIKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(52205..53356)
                     /locus_tag="PC407_000355"
     CDS             complement(52205..53356)
                     /locus_tag="PC407_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2495837.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            53626..54267
                     /locus_tag="PC407_000356"
     CDS             53626..54267
                     /locus_tag="PC407_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDF2495838.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CTNLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTVDGANTFERDWSKVNK"
     gene            54293..55837
                     /locus_tag="PC407_000357"
     CDS             54293..55837
                     /locus_tag="PC407_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2495839.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AVFELATVNKSYTELKEILFSNSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(56054..56680)
                     /locus_tag="PC407_000358"
     CDS             complement(56054..56680)
                     /locus_tag="PC407_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2495840.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(56815..57531)
                     /locus_tag="PC407_000359"
     CDS             complement(56815..57531)
                     /locus_tag="PC407_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HDF2495841.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            57946..59160
                     /locus_tag="PC407_000360"
     CDS             57946..59160
                     /locus_tag="PC407_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2495842.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            59779..>60003
                     /locus_tag="PC407_000361"
     CDS             59779..>60003
                     /locus_tag="PC407_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495843.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKP"
BASE COUNT        19540 a        10639 c         6524 g        23300 t
ORIGIN      
        1 ttagtaatct tttataatct atactatgat aaatgtatat atttttttag gaggtattat
       61 aattggatac taaaaaagtc acattttctt caatatgcat agttattaat attgtcttag
      121 gtatggttat atctatgata aatataccat tattattttt agatacagtt ggtactgtac
      181 ttggggctgt cgtattaggt cctttttggg gtgctcttat tggtgggtgt acaaacttag
      241 ttttaggtgt tatatcaggt cctacaaata taccttttgc tcttgtaaat attgttcttg
      301 gtcttattgt tggatatatt tctaagaaaa aaggttttgg ttataaagaa gctattatta
      361 caggagtaat tttatctatt gtttgtcctt taattggtac tcctatttca gttttattgt
      421 ttggtggttt aagtggaagt ggtgctgatt tattggttgg ttttttagtt cagtctggtc
      481 agaagatatt tactgctgcg ttctttccaa ggattcttag taatatagta gacaaacctt
      541 tgtcttgtat tatggtggta tattttataa gtaaaatgcc taaaggtttt ataaaacaat
      601 atagtaaaaa tacttctagt tcatattaat aaatcattta ttatgtaaga tttattactc
      661 tttaaaatgc tatatactca tttttatata acatttttat taataattta attagatagt
      721 atttgtatta ttttaattaa acgtaacctc ttgatataaa tttgagctat aaatctatat
      781 caagaggtgc tattttattt ttaatataat taatattcta ttacgcaaaa aatgcatata
      841 tttcattatt ttatggaaaa tattacttca tttaactcta tatcaaactc attaagtaaa
      901 aatctaatta tatttaattt agcctctgta tttaaatttg tttctaaata tatattttct
      961 gatattttaa taccttttct taatttatat acatctctag acttaatatt atctataaaa
     1021 tctcttttat aagtcttgtc tacaatactg ttaaacctta cagaatccaa atcatataaa
     1081 gtaagagcaa cttgttcata aaattctgtc caatttttag ccttatatga ctctcctaat
     1141 atactaaatg atactatact ttcatttgta aaattaacat catcatcctc taacgtaaat
     1201 atattttcac tgttacttgg aggttcatat gtaacacttg gatatttcca aatttctaca
     1261 gctctatcta gtaatatctt agttctattt tttatttctt cttcattcca tttatctata
     1321 gatgaaatat atttatttag ataaagccta ctatctttaa atccatcttc catttctttc
     1381 ttttcttcaa atgttttatt acttagtttt gaattatatc ctgtcaaagt taaatttcca
     1441 attgtatgta aatatttatc atgtatctcc ttatagttat ctcctagtga ttccttccat
     1501 ttatttgtta atgtctgtgg cattatatgc tctatattta gcatattatt attgattaaa
     1561 ttttctaaat caacaatttc tttattatta taattctcta acctctcaag tatatataac
     1621 ttattctttg acttagtatt atatatatct ttagtttcaa aagcatttga aaattcactg
     1681 tcagtaggaa atctttgaga tgatttctta tttataaaaa tatatcttaa tatattagaa
     1741 tagttttctt tataatcact aaatttttta atttcccttc ctaagttcat aaatacttta
     1801 ttcagtgcat tcggaggtac atcacatact attcttctta atataaaaca ttctactaaa
     1861 ttaagggatt ctacaagtac ttttttattt attatacctt cattataatc attaaataat
     1921 tctaatataa acggatatga tacaaatatt tcaagtttgt ttatcctttt caaaactttg
     1981 tttacatcat tatcatcagt acatgaataa gatatctctc tataatagtt agaaaattta
     2041 agtaaatctt ttaataaaat ttctatatct ataccattat ctgatacata ccttttaaaa
     2101 ctaaaatata ctttattttt attcggtata cttctctcct ttatagtcaa atagtctctt
     2161 ataaagctac ttacgttgct acttgtattt ttttcaattt tataccaata attattataa
     2221 aattcctctt gtaacttaga gcttctaccc attaacacaa agtttcttac tctatcagct
     2281 tcacttaaat ctaagccagt tgaatttaga ctttcaaaga ttaattgagg gtcatcttca
     2341 ccatttttta actctatttc tactatagtt aatttctgta tagcatcaaa caaatcatct
     2401 attgatatat tatcttcttt tattctatta taaaaatata aataatttga aactatatta
     2461 gaatttctat ctgtattcat ttcttcttca aatagttcat agtaagcttt tctatcttct
     2521 gctattggtt ttaactttat ttttccatct ttatctcttg agtgcttgtt tataagataa
     2581 cactccttaa tctgttcttc tgttgtaata attccttttg attctccaaa ctcttttatt
     2641 atttgacaca tggcaatcaa taatattgac acagtagtca ctctttgttg tccatctatt
     2701 attaaatatt cttttcccat actatatata gatacaactg accctaagaa atgagttcta
     2761 aatccagaat ctattatatc ctctaaatca tcaaacaatc ttttacattg ttctttcttc
     2821 cagttataat ttctttgata tacaggcact acaaaacttt tgtttgaccc ctctaaataa
     2881 ctaagaaatt caatttctcc tgctttcata ttaactcccc ctataagtta aataaattcc
     2941 attcataact ccatgtatct atatattata tcaaaatttt gactcataaa tgtatatgaa
     3001 agccttatta tatacctttt tatacctatt tttattttat taaagtaaat acatgattat
     3061 tctataaaat atacaaatac ttaattttaa aaattattat ttaatcttct tcatgagtaa
     3121 ttcatagtta tttatcaatt aaaaagagat attgcataat tattcaatac ctctctttgt
     3181 atatttacat tatccatatc tagttatcta tttcaaccca atattagact acctactaat
     3241 aaatttattc tctacataat taacaataat tttgcactaa ataacatcat tagcctatct
     3301 ctgatgcatt ttctttaatt ttatttatag ttttaatcaa tttatttctc tcttcaatat
     3361 gattttcaaa taaatcaatt atacttccaa cagttctaaa tgggtcttcc atcaacactt
     3421 tgttatcctc tataaatcca ttcttgacta cataatcgac tataagttta acaaaatgta
     3481 tttgcttagt attaaaactc ttattattta aaaactctga aaaagcttca tttgcagctt
     3541 ctctatccaa tcccactagt tttctcacta attgacttac tggagtatct ccaaactcct
     3601 ttttataatc atcacaatta cctaactgct ttaagagtat ttcctccaac gtttcaacat
     3661 cttgctttgt tatcttttta ttatttctta atttaaatat tgctaattca tctttatggc
     3721 tatttaaata atactctacc ttctttttat aattcttaag attgtttgca ttatatacag
     3781 aatcatttct atcttctgct acaatcatat cttcaaaact tgtataatat atcttttgac
     3841 tagacttttc aagatactta ataagctctc ttaatgatat acgtacttct tctacctcaa
     3901 atatatctga atcttcccaa aaatcctctg tcattgcttt ttcaatagta tatttttgtt
     3961 gctggatctg tggtatagtc ccaagtttcg aaagtttttc tgctgtaacc attacatgcc
     4021 ttatcccttt acttgcatta ttattttgta agtatgcaag ttgtatatta tacataacta
     4081 catcaaatct tttagccaat tcatcatcac tatatgaagt tatgagtggt gatatatatt
     4141 ctcttatctc attttgtgct actgctccaa tattgctcca aacattttca tttttatact
     4201 tatgtacata acttaaattc atcctaacta tataactatc ctcatttaaa gcttttacag
     4261 aagttataag ttcttctaac aattcttttc taagttctgc atacttacct tctatatatt
     4321 taatatcttg aagttcttta accatactta cctttatatt aaataatttt tcagtaagtg
     4381 tttctgcttt atttccctca aatcctttag gattaaattt aaaaaattca aaattattac
     4441 aaaaatcaaa tattaagaat ctttccttat ccatatcaac acctaataaa tctggacata
     4501 atctagttcc tctacctatc atttgccaaa acttagtctt acttctcact ttcttgaaaa
     4561 aaacaagatt taggatttca ggtatgtcaa ttcctgtatc aagcatgtct actgaaactg
     4621 ctatttgagg taatttattc ttatcagaaa aatcatctat tatagaatct acataattta
     4681 tactatagtc aactgctttt acaaagttcc caccatactt tggatacagt ttattaaatc
     4741 tttcaactat tgccctagca tgctttgtat tctttgcaaa aattatagtc ttaccaatct
     4801 tttcttctcc ttctattttc aacccctctg tcattagctt atttaaaact aaatctattg
     4861 tgtcagaatt aaatagccac tcatttactg cattattccc tatttcgtct ccaatactct
     4921 catcatcatt aaatgtattt tcatattctt ccttctcttc atcagaaagc tcatcatact
     4981 taattccatc ttccataatc ttagtcttaa actcaatact agtataatct actaaatatc
     5041 catcctcgac tgctttatta tactcataag catatgtagg tactccattt tccatgtcaa
     5101 aaactgaata agtattttta tctatctcat cctttggtgt agcagtaaga cccattaagt
     5161 aagaatcaaa ataatcaaag attgacttat actttttata aatacttcta tgcgattcat
     5221 ctattataat taaatcaaaa tgaccaggtg taaacaatct ctttccatcc ttagacttcg
     5281 tatcatcaat tgcattcatc attgttggat atgtagaaaa tatcatccta gcatcttctg
     5341 ggctgtcttt gctatccaat aaattacata acgccaagtt aggtaatagc ttagtaaaac
     5401 tttttttagc ttgctttacc aatgcttttc tatctgctag aaataatata tttttaatcc
     5461 aattatgtct tgaaagtaca tcaactatag atatagcaat tcttgttttt cctgtaccag
     5521 tagcacatac caaaagagtt ttgcgttgct tattttcaag agcctcacat acagccctaa
     5581 tagcttcttt ttgataatat ctattggata tttcatcatt tattgttaca gaacttaaag
     5641 acttcttact ctttcttcta tctatcataa gttgtaattc tgatttttta taaaatccat
     5701 aaacttttct atctgaatag tcatcccaaa tatacatatc aaaaccattc gtcaaaaata
     5761 ttactggtct ttgactatat ctcttttcca aacaatctgc atataattta gcttgttgtt
     5821 gaccaatttt cgggtctttg ctcgttcttt tcgcctcaac tactgctaat ggcttaccat
     5881 tttcaccata aagaacataa tctacatatc ctacaccact ttcattaggc ataccttgta
     5941 cttctatttc ctcccctata tcttttccaa attcccaacc tgcaagcttt aattctatat
     6001 ttatatatct ttttcttgtc tcaaattcgt ttacttcgtc tatcttgaaa tcgtagttta
     6061 cagtattaac ttttctcttt tcagttagtg actttcttaa tttctcattt tcttttatta
     6121 cttcttcaag ctttttatct tttgaactta atctctcata aagattttta agctcgtctg
     6181 gtctcgtcct tttctcttcc ccatgaagta ataaatcttc attaaagtct tctgctgtat
     6241 attcatctga ataacaatag tctatccaac taacaaattg atgcaaattg ttaagtgaaa
     6301 gtattgcttc tcctctctct atgttcgcac tagtatgtac agataaatta cctaatttaa
     6361 ctatatattt taataaaggt aacatatcat agtctataag ttctataaaa ctattattat
     6421 gaatcaacgt actaagatta tcttgataag gcaatactaa atcactatca ttagcatata
     6481 gccattttac tgcaagctct aaagctcttc ttgttatagt tgcacaagta gatgggctaa
     6541 ctagtatact tttttctgct tctaagcaag cagtattaaa actattgaaa tttttcttct
     6601 tttgtaaaaa gtcaaaatta cccaaggctt ttctccccct tttgtccctt agttaaaata
     6661 aatctctcta aaaactattt cttacagctg tttcttatac taacaaatac ttcttcattt
     6721 tctgtaaaca attcgtacat atcttcaaac tgtggtatat catatccatg agaatcatca
     6781 tttctcaatt ttctaagtgt ttctataact atagaagaat ttttaggtaa taaattgtga
     6841 tactcacaag ctaaaaggta gtcataaaag tttttagatt ttatgtttaa atcaattgat
     6901 ttataataca acattgcttt tgttaattct aaaaatgaga ccatatagct tttaaaacct
     6961 tcttttatgt atctagccca taactcaggc atttcttcct tatcatataa gattttgaag
     7021 ttttgcaggt catataagca agaaccaaaa tccctattta cttcttctat tgagttccaa
     7081 gataatttgt ttctaattac catacatgtc cctccaatct aagatatcta ttacatcact
     7141 gttttcaaaa tagaatttat ttatataatc cttaaaatct ttagattcat aaataacttc
     7201 accttctaaa atagcttttt taagtgctat tgtataatta tctgtaacaa tttttaggtc
     7261 tacatcaatg tctaatatat ttcttagagc ttctgtctta gttgcaagtt ctgtaattgg
     7321 tacttttttg ataggaatcc aagctatgtc tatatcgctt tctgatgtat gtttatctgt
     7381 tccataacta ccaaatatta tgattgctag agtattttct tttggaaata catttattat
     7441 tgagttttta atctcatttg gtatatttct catttaatca cctcttgttt attaggtaat
     7501 attgcttaat tgaatttata tccataatct tttaatctat ttatacatat ttctctttct
     7561 tcatcagtaa acaactcttt atacttttct tttaatacta atacttctaa tgataaatct
     7621 aatctcttac actcccatag ccttgtaaat ccttcagtag ctccttcttt attgattaat
     7681 tctttagcag ctaataaggc tcctttgtta tttatcagtt gtaaaaatct tgttggctta
     7741 tagttacatt gtaaatcagc ttctttatag atatttacca tgtcatgata taaatctagc
     7801 tctatttgat tcatagattt tattccccca ttttaatact tgtatttcag ttgaacaatt
     7861 ctcctttaaa agctttttgc attaatgagt taaagttatc ttctagttct tttaagctag
     7921 tttccatttc aaacttcaaa gagtttactt gtttaacgaa gttggcaaat tgattttgaa
     7981 gtctaatagg aggtacaata atatttattt ttctaacagc atctttagtt atcgtttttg
     8041 ttacagctcc tcttactttc tcttttattt tactttccat ataggatgtt agtaaaaggt
     8101 tatataagaa tatatgattc actttatctt cattaaatct aagcataaat tgattcatag
     8161 ctaaagatac tggtttattt aaaaatggca ttaaataaac tttacctgca ctacctatct
     8221 tatttattat aacctcacca ccaaacacct ttgatttttc aagataatta tatgcatgtt
     8281 catcgatata cttaacacct ttttcaaagt tattattttc taaatctgta gttcttacca
     8341 ttaaggcata accttttgta tctaaaagag ttacattatc tcttaaggtt tcataactac
     8401 catttgaatg ataatcagtt aaaacatcta aatatttccc tatttctgaa atttcccagt
     8461 ttttttcgtt ctttgatggg gtaccaaaca tctcaataaa tcttgattta actaattcat
     8521 ccaatacttc tatttgctct tttcttttat ctattaactc ttgagccttg tctaaaacac
     8581 gcactacctt ttcttgcact tttaaattat acatgcgtat ttttatatta gctaatcctt
     8641 cattatttaa agctgattgt gtagcacctt tacttaacct atttttttga tttagaaaat
     8701 aactactatt taagtaatgg tataaatatt gaggcaatat ttcttttgat ggttttaaaa
     8761 cataaaagcc agtcgatact attatatttt tattagtttt attaataact agtgttttct
     8821 ttgtgtcctt catttttgca aatataactt ctcctatatt tacagtttga tttgctctag
     8881 aaggtttatt ttcataagta actatttcta gattgcttat cttgttaaaa tctaagtttc
     8941 cagtactaag atatgagcgt tctccatcaa acttttttac attttccctg cttatactaa
     9001 caacatctaa aatttttgct tcatcaacca tactctacac ctctaacatt ctttctagtt
     9061 cctctaatcc atccactatc tctttctcta acaccttaac cctacctata ataacactag
     9121 gagcatcata aactacttcc tcatacacta cctctttata cttattaata gataaatcat
     9181 acccattctc tcttatctct tccacatcaa caaaaaagct ttgctctgtt ctttttctat
     9241 ctacttcccc atcaagatta tcaaatctct taataatatc ctctatatca ctatccttta
     9301 tctcatttct cttatcatcc aaagaataac catcagcttt catatcatag aaccatacct
     9361 tatcagttcc acctgtccct gttttagtaa atatcattat agctgtagat acccctgcat
     9421 atggcttaaa tacaccactt ggcattgata ttatagcttc caatttatga ttatcaacta
     9481 tttcttttct tatcgattta tgtccacctg ttgaaccaaa caatactcca tctggcacta
     9541 tacatgcgca tcttccacca atttttaaaa ctcttaaaaa aagtgccaag aataataact
     9601 ctgttttctt tgtcttagtc acttttaata aatctgctga cactgcttca taatctaaac
     9661 ttcccttaaa aggaggattt gctagcacca atgtaaactt atctttatct gtattaacct
     9721 ccgataaaga atccttatac tctatattag gattgtcaac gccatgcaac atcatattca
     9781 tagcacctat tctaagcata gttctatcca tatcaaatcc atagaacata ccattattaa
     9841 aatgctcctt cagcccttgc actaagaaaa gacttgaatg attatccctt aagtactgtt
     9901 gtgatgatac taaaaaacct gccgaacctg ctgccgggtc tactataata tcttctggtg
     9961 ttggtttaat aagtcttact atcatatcta ttatgtgtct tggagtccta aattgaccat
    10021 tagtacctgc tgtagccact ttggataata aatactcata caaatctccc tttgtatctc
    10081 tatccttact taattctaaa ttactaatac catcaactat tttagataac ataagaggcg
    10141 taggaatttt aaatattgca tctcccatgt actttgcata agctgattct ttatttccat
    10201 gtaatttttt tatgaaagga aacacttcat tttgtactat ttcatacatt ttttctgagt
    10261 ttccttcatt agaaaactta ctccatctta aatgttgatg ttcatcatca aatatctttt
    10321 caaaagaaac acccaaaaga gttgcttctg cttcattttt tgtttctact tcatccaatc
    10381 ctttaataaa taaaagatat gtaaattgtt ctataacttc taatggatta gttaatccac
    10441 cagtccaaaa tgtctcccaa atcttgtcta ctttactcct taattctcct gtaatcatat
    10501 atgactttcc ctccagtgtt cataaaataa tttaaatata tattttagtc gtacgcctta
    10561 atagttagtg tcttatgtta aatccaagtt tttactacac aattattaaa ttatttttcc
    10621 catttacata ttttagctaa aaaactaatg caatactctt attttatgat tattatatat
    10681 accatacaaa attattttat catatatcca tcatagaagt catctacctg gataactctt
    10741 accatattat aactttttaa tattaattta aacttagaaa gaaatttttt atttaattat
    10801 atcatcttcc ttatttcaaa tttctcaact acaatcctaa tcaaaaaata aaataagatg
    10861 ctgtctcaaa atagatataa tcttcttttt tattgtcaga atgatgaaaa tatatttgat
    10921 ttcacacaaa aaagagtgcc tcatgaacta aaaagttcat tttgagacac cccttaattt
    10981 aatcttactt attctttccc tttattatta acacttcact gtccctattg cttttcctac
    11041 acatccacaa acttccatcc tactcttaac aaaatcttta actgactcta aacctttttt
    11101 accatatttc ttagggtcaa ttacctctgg gtcagaatta agcacatcct taaccccatt
    11161 gctataagca atccttaatt cagttgcata attaactttg caaatacctc tttttataga
    11221 ttccttaata acctcatctg gtattccaga cccaccatgt aatactaatg gaactgatac
    11281 aacctctcta atttcagata atctatctaa atcaagcttt ggctctccct tatatagtcc
    11341 atgtgccgta cctattgcta ctgccaaaga tgatacccct gttctctcta caaactcttt
    11401 tgcttctaat gggtctgtat atgctccacc atcaccacca tctaaatcgt cttccttacc
    11461 tccaactttg cctaattcag cctcaacagg tatatctgaa ggcatacatg catctacaac
    11521 agatttagat attgaaatat tatcttcgta actttcatga gaaccatcaa tcataataga
    11581 agtatatcca actctaagtg cctgcatagc tagtccaaag cttgaaccat ggtctaagtg
    11641 cattgccact ggaacacttg ccttctctga tgctaccttt acattcgcta aataataatc
    11701 tagccctgca tactttattg ttgaaggtgt tgtttgcaat attactggtg atttaagttc
    11761 ttcagcagcc tctattactg ccataaccat ctccatattt tcaacattaa atgcacctac
    11821 tgcataatgc ccttcttgtg catctaataa tatttgttta gttgtcacta atgccataat
    11881 ttctcctcct atatgtttat actgatttga tttcttcgtt atttaatttt gatactattt
    11941 ctctacttgt tttgctattt aataactcat ttaaaaattc ttcattcatt aattttgaag
    12001 atagttgaga taatgttttt aagtgtaaat tatcaccatt atctgtagct gatatcataa
    12061 atataatatg tgccaagctt ccatcttctg attcaaagtc aaacccttct tttgatacac
    12121 ctactgctac acgtggtacc tttactgcat ctgtttttgc atgtggtatc gctatcccca
    12181 taccaaaacc tgtagatgat atttcttctc ttttaaggat ttcttcttta aacttctttt
    12241 tatctaaaag aacaccagat ttatcaagta ttccaatcat ttcatcaatt acatctgttt
    12301 tattttttcc cttaacatct aaaattactg tttcttcgct atataaagag atattattag
    12361 atacgttttc cgattctgca ctttcatttt tatgtccatc ttttgttgca tttgtatttt
    12421 ctatagtatt tttctcattt actactttag gtgtaaccat catataaagt acagcaccta
    12481 ctaatgaacc tacaaatata caccctaccc ataaaagtgg cttattgact aatcctagaa
    12541 ccaaaaaccc tccatgtgga gctggtactt ctattttcat tatataagtt aaaattgatg
    12601 atattgatga tgcaatcata agtactggca acacttttaa tggatttttt gctgcaaaag
    12661 gtattgcccc ctctgttata tgagtactac ctagtatata attgattaat cctgctgctc
    12721 tatcttcttc tgtaaatcta tttttaaata aagtagatgc tattgctatt accaatggtg
    12781 gtgttataca agctgcagat acacctgcca taaataaata atttccctgt cctagaagca
    12841 tagttccagt tacatatgct gctttattta ctggacctcc catatcaaat gctgacatac
    12901 atcctattac tattcccaaa attataggac ttgaattttg taaccccgat agaaagttca
    12961 tcatagcatt gttaagtgct gaacatggtc caccaagtaa caacattgct acacccacaa
    13021 gacctacgct gacaatagga attataaaaa tcgatttaag accttctaat tgtcgtggta
    13081 attttcttaa caaatgtaca aatagtttaa ctaagtaacc tgctaaaaat cctgatatga
    13141 ttccacctaa aaaccccgaa cctgtatcaa atgatatcat tcctccaaca agtcctgcca
    13201 acataccagc tcttcccgat atagattgag ctataaatgc tgccaataca ggaaccatca
    13261 agttcataga atatccacca acagatttta atgtagctgc tatttgatta tattgttctg
    13321 aatttgggtc aaatgaataa atcccccata aaaatgatat agcagtaagt actccacctg
    13381 ctactactaa aggtaacatg tgagatacac cattcattaa gtctttatac aacttttgac
    13441 caaacgataa atttccttct tctgcaattt tactgcttga taagtttcct tttctaatag
    13501 ctacttttcc atcaatacac ctttgtataa gttcattagc cttatgtata ccttctttga
    13561 caggtacttc tattaccggc tttccattga atctgtccat atcaacattt ttatcacaag
    13621 caactattat tgcatctgcc atttcgatat cttttgctgt taacttattt tctacaccta
    13681 tagctccatt cgtctcaact tttatgtcac accctaattc ccttgctgaa ttttttaatg
    13741 cttcttctgc cataaaagta tgagctattc cagttggaca acctgttacc cctagaatca
    13801 ttttctttcc catctttata ccacctttct gactcttata ttttttatat aatcttccac
    13861 attctccaaa ttacctatac cattactttc agccacactt gctcctgttg ctgctgacct
    13921 ttttaatgct tcttcaatat gttctggatt ttccaaccat atacttaaaa aagcagctaa
    13981 tgcagaatca ccagcacatg cagagctgac tactgtaact ggttgagcac tagcataata
    14041 aattgatttt ccattataaa aataagaacc tttttctcct aaagttagca atatattttg
    14101 agcacctttt aaatgcagta atttaagtac atccttaata tcttcttcat ctcttacaat
    14161 aattccaaat atatctttga tttcttcatc atttggtttt atcaaatatg gattatactg
    14221 tagtaactcc tttaattttg gtgaacttat atcgagtatt gtctttatac ttttattttt
    14281 acaaatttta aatatatcct catagtaatt atcatcaata ccaggaggta gacttccact
    14341 tattgataag taacttatat ctgctaatga ctgtatctta tctagcatat ctagtttttg
    14401 tttttcgttt acatatgacc cagagtttac aaatttaaat tcatcacttc catcatttaa
    14461 aaatatattt atacgagttg tatcttctac ccaagtaggt aatacgtcaa atcctctctt
    14521 ttcgctttct tcaactatat attttcctga aaatcctcca aaaaatccta atatcttaga
    14581 ttctatacca taatgaccta gtacaaaagt tacatttaat ccctttccat taggagaata
    14641 tacagcattt gatgtacgat ttactaattt tctctttatc ccattagtag atatattcat
    14701 atctattgca ggattcgtag ttaatgtata tatcattgtc ttcccccttg cttgtgctgt
    14761 gtatgttttc attataataa aaaagtctat gaaaatcttt tcacaaatag aaaatcgatt
    14821 ttcatagact tttcaattta attttcagaa ttattatatc tgaccatata atctataatt
    14881 atcctagata taatatacaa gggcaatctg gaagatactt catattcaga aattgatata
    14941 tgttcatgtt tgtatcctac aatatataaa tccgaatact ttataagttt tgcactttca
    15001 ctacaacagc aagttataac cttagctcca actaaattgg cattctgtgc tgattctaca
    15061 agctctatgg tatttccatt caatgaaaat atgattataa gttcgtcttt ttttattgat
    15121 ttagatttta ttttcattat gttggggtca tttataaaaa caacattaaa atcaagtaat
    15181 tgcaatttaa gactaaactc ttttgctaca tattcagtaa gccctcttgc caaaatatat
    15241 atcttactag atttatttat agtttttatt atattgatta gtgtagttaa agatatttgt
    15301 tctataactc tctgtgcttc tataaaggat ttatttatta tatcccccat attttgtata
    15361 tcatcatttt catcttttac tgttaatcta tacctaagtt cattgaatcc atttaaacca
    15421 cattttctaa ttgctcttga aacggttgct ggagatgaat atgtttcatg agcaatctct
    15481 actatagata attctggtaa tattgattca ttttcattta taaatttaat tatttcaagt
    15541 tccgtcttcg ttagattttc agaattactt ttgtttagct caattatcat atgttatctc
    15601 cccttatata agttttattt gtctaaattt atcgagtaat ataatatatt acctcttaac
    15661 tacttatttt tatatacaat taagatttag tcaatgtcta tatatgtaaa tttataatat
    15721 aataatttta tgcttcaatt aattatgtaa ttattagagt attttgtaat aaaaattgga
    15781 gttactcaat tactgaaact gagtaactcc aattcttaca gcctcaaatt aactcgaatt
    15841 attgaaaatg attcataaac attatcctat actctcactt atcaatttat atacttgagt
    15901 aatacctttt tttattaaca gaaatcatct tactaacaat tgaaattgta gttccagtac
    15961 ataatattga aaatatagtt ccaaacccta aatttccgcc taaatataca cctaaaatta
    16021 aaaatattaa atctgctata gtttttaaaa ctttatatgg atacttaaat ttgccctcaa
    16081 tatagattaa tatagcatct aaagggctca ttccaatgtc aaattttata agcattgcaa
    16141 atccactaca cataagtata tgtccaatta taaagctcat tattctaatg ataatcccta
    16201 aactacttat attaaaatct aataataaat atatatacaa atctataaat attcctgcta
    16261 ctaatacata aacacagctt cctaatccca aacttttttt attcaataac aatgctataa
    16321 gtaatagagt tccattatat attagccctg ctattgtata acttatacct aactttatat
    16381 gtataccctc atttaaaagt gtaattgcat cactaccaag gcttgactga agtataagag
    16441 atataccaca tcccagtata tttataatta aaattacaaa tataaatttt tttatgacat
    16501 tcataattaa taaaccacct tatttataat tataatccct cttataaaac tgtattaata
    16561 acatagtatt tataagaggg attatttata tttaaaaact atctactttt tcttttaatt
    16621 cttctccatt agttcttatt acatttttat accaataaaa actatctttt ttgtaacgtt
    16681 ctttgcttcc atttccattg ttatctatat ctacatatat aaatccatat cttttttcca
    16741 tttcttgaga tgaacaactt actatatcta ttggcgacca catacaataa gctaatattt
    16801 cggttccatc agctatagct tcacctattt gttctatatg ttttcctaaa taatctattc
    16861 tataactatc atgaactttt ccatcttcaa atttatcatg cattccaaat ccattttcag
    16921 ctatcattat tggcttttga tatctatcat aatattgaga taaagtattg tataatcctt
    16981 tagggtcaat tgcccacccc caaggatttg cttttaagta ttgatttttt acattatcaa
    17041 ttggattcat ggtattttta cttgctgata ctgttgtaga gtaatagtat gatagagcta
    17101 gaaaatccat agtattctct tttaacactt tatcatcacc ttcttccata actatattta
    17161 tgttattttc tttgaagaat ctcttaatat aagctggata ctcccctcta aaacttacat
    17221 cagtaaaaaa atattgtatt ctatttcttt ttaaagcaaa tactacattt tcagggtcgc
    17281 aatcttctgg ataagatgta caatctgcca tcatcatccc tatcatcata tctgaattta
    17341 tttcttttcc aagcttctta gttaaagctg tagccatgaa ttgatgatga atagcttgat
    17401 atttggcctc ttctacatca tctacctggt ctacacaaaa tgcaactgaa ttaaatgatt
    17461 catgatacat taaatttatt tggtttataa ctatccaata tttaacttta tctttgtatc
    17521 tatttaatat taattctgca tatctaacaa agaaatccac tacttttcta ttagaccatc
    17581 ctttatattt taatactaaa ttcaaaggag tctcataatg gagtagtgtt acaataggtt
    17641 ccataccatt atctattact tcatttataa gattatcata aaactttaat ccctcttcat
    17701 taggctctaa atcatctcca tttggaaata ttctagccca atttattgaa aatctaaaac
    17761 actttaaccc tagttctttc aaaagcttta aatcttcttt gtatgtatga taaaagtcta
    17821 ttccattacg cttaggataa tacccattat tatctttcat agcaaacttt attttatcta
    17881 cagtcatatc agactcgtaa tgtttagata tatctctatc tttatcatac atatgtacat
    17941 cagctaagca ccaccctttg ccaccttcat tccaagcacc ttctaattga ttagctgcta
    18001 cagctccacc ccacaaaaaa tctttaggaa acttactcaa tttaatcact ccttatttta
    18061 tttatttaaa tcatttacta ttatttacct tatattctat agactttact attcctacta
    18121 aagacatagt tatatttaac tcgctgaata ttgtcataag agtatcttga gcatgtgcaa
    18181 ataaaagtga atgtgctctt ttttctccac tagcttcccc ttgtataatg ttagtttgag
    18241 ctctatgtgc ttctgtcata aactcttttg ctttagctag ttttaaatca gcatttttat
    18301 aatcaaacct ttctaaatca ttaagagcat gtttaatttc tgttctagcc tcacctgcac
    18361 gaagtattat ttgcattgct acttctacta attcttcatc tattacatgt tcttcagttg
    18421 tcatatttat aatttccttt caacatctaa atttaagctt cttgactctc taattttagt
    18481 tcttcttttt cataagcttt aaaaaatgga taccatatca tgaatagcaa tactatattt
    18541 attgctaaaa caataagccc tctaacatct tgatttacca taaaagtaga tataccaaaa
    18601 ggaagatacc ataaattaaa caccttactc ggaattgtca caaatcccat actcattgat
    18661 aagtaagtag ttattgatat tactactgtt cctatccaca taggtaacat taaaaatggg
    18721 ttccatgcta ctggagctcc aaacattact ggttcattta tgttcattat agaaggaacg
    18781 atacaggctc ttccgattgc ttttattctt ccagctctag ataaacacat cattagcact
    18841 agtggtaatg ttgctcccca cccacctaaa gttatccaac ctgtttgaac aacttcataa
    18901 gtatatatat tagttgcaag ccctcctgat gctacagctg ctgcattagc tgcaattgca
    18961 ctaagctgaa ttgcataagt tattggggat aatacccatc cacttatacc taaagaatat
    19021 aaaaatacta ctaagaagtt taatagtaca aatccaggca atgtttgtgc tatatctgat
    19081 actggtttaa atatcctaac tattatttca aacatatcaa agttaagtcc ataaattagt
    19141 ccccaaccta taaacattat tactgctata ggtaacatac tatcaaacca cttacttaca
    19201 aaatcaggaa gtgctgaatc ttcaccaaaa aatgagaaag acccaaataa agacattatt
    19261 aatccggaga ataatcctgc aactagtgct acaagcattc cttctcctcc aaatctattg
    19321 aagttaaata tagctccttc attcgtaatc tctggacgaa tcatcattaa cattaatcca
    19381 gcacctgcaa gaccagctac tacactcata ctttcaaatt tcttattctt cattatttcc
    19441 ataggtaata aaaatgctat aaatagtcct aaaagtccaa atgtatattg atttattgga
    19501 cttaaatcag gtaagaaact tatgtaattc ttcaagacag aaactatagt tactaaagaa
    19561 cctacaaata ccataggcaa aactttcatt atagttgatt gaactgattg tacccatata
    19621 ttcttagtta tcttatttac aacaggagta aacttttcac ttaacaaatc tataaattta
    19681 ttcataaaca atctccccat agatatttta tttgtttaat attaattcta atgcttcttc
    19741 taaagccttt acaccatcta atcttccata tgctgtttta gatatacatg ctactggaac
    19801 attatattca tcagcttttt tttgtatttc atctttcata taatctaaat gtggtcctaa
    19861 taataaacaa tctatttctg gtaaatattt atctatctct gactcacttc tagcttttat
    19921 atctgcttct atgccttttt tctttgcact ctttctcata ctagttgcca taaaacctgt
    19981 acttgctcct gagccacatg ccaacaacac ttttatattc ttcataacaa tacctccagt
    20041 atttttattc attaatttat ttcttaatga atttttattt gatttgattg tatttatatt
    20101 gtacttccta aataatttcc tttcacataa atatatttcc taataatagg aaatcattta
    20161 ctcttaacca gacacataaa tatatagtat aatgtacatt ataatacgta cattcaatta
    20221 tataaatatc atatcataca gattttattt taaaactatg agagggaatc taatgaaaac
    20281 atctaaaaaa gagttatttt taaacttcat aaaaacgcaa catgattgga tagattcatc
    20341 cacattagct aattatctta atgtatcaac acgttcgatt agaaaatatg taaatgaaat
    20401 taattctact ggtgaattta tattatcctc taaaaaagga tataaagtaa atctcaataa
    20461 taactgtcaa accaaggttg atagttcaga aaatatatca cctgataaca ggttaaattt
    20521 aattttaaaa gaacttatag ttaatagtaa tggaattaat atatttgatt taagtgaaga
    20581 attgtttgtt agtccagcta ctattgaagg ggatatagtc aaagctaata aatttatagg
    20641 aagttataat ttaaagataa agcaatcgaa atttttattg aaactaattg gaaatgagtc
    20701 tgcaaagcgc aaattaatga gctctataat atttaaagaa acgggaagtg attttttaag
    20761 cttatttgat gtacaaaaaa tatatcaaga gtataatttg accaaactga aagaaaatat
    20821 aatatatata ttaaaaaaat acaatttgtt tataaatgag tacgctataa ataacatact
    20881 tcttcattta atgataacta tagacagaat aaaaaagaat aactacatag aatctgtcga
    20941 agtagtaaac tacatagata ataataatga tataagcatt tcaagagata tatcaaattt
    21001 tttagagtct gaatacaatt taactttaac tagtgcagaa ttatactact tagtatttca
    21061 gttaacaaac aaaaccacag tcttaaatta tcatgaaatg gatactaagt cattgccaaa
    21121 ttatatagat gaacattttg ttaaattaac taaaaaaata attaaaaatg tatatgactt
    21181 atactttata gatttatctg atgatgaatt tgttgtaaaa tttactcttc atgtaaaaaa
    21241 tttaatcagt agggctaaaa ataatcaagt tttgagaaat caaatacctc agaagttaaa
    21301 agattcctac cctctgatat atgatatatc tgtttatatt tgtaatcaaa ttcaaatttt
    21361 agaaaacata gaaatagatg aagatgaaat atcgtatata tctcttcatg taggttcatt
    21421 ttttgataga caaaaattac tagaagataa aattctctgt gcattaatta ctccaaatta
    21481 ctatgattta caattcaaaa tagtaagaga cctcgagaag agatttaatg aatccataga
    21541 aattattcaa attttttcag atactcataa tcttgatttt gacaataagg tagacatggt
    21601 tattactact ttaccaatta gtgatagatg ccctatacct tttgtttatg taaatcctta
    21661 cctaaacaga aaagattatg ataatatcca aagtaaattt attcagatta aagatagaaa
    21721 aaatatactt actgtacaaa atcatttaga aatgtacttt agtgaatctt tgtttatgaa
    21781 aaacatatat ttagatagtg ctaaagatta cataaaattt atgggaaata ttctgtacaa
    21841 aaataaatat gtcaaaccca attatataga tgatgttctt ataagagaaa aaatgtcttc
    21901 aactgcattt aataacaatg tagcaattcc tcatagcatg aaaatggatg ctttaaaaac
    21961 tggagtatgt ttaattgtta acgataaacc agttaaatgg ggtgaggaaa aagttcaaat
    22021 tatagctatg atacctataa atgaaaaaga aaaagaaaaa ttcaattaca tatttgaaag
    22081 ctttattgaa atattatctg agtggaataa tgttaaagaa cttactaagg cagataacta
    22141 cagtagtttt atgaatagaa tagcttatct tattcaaaat atctaaatcc attaatttat
    22201 ttccaatata tgtttcttaa gttattaaca caaaatagat aagctgtcac actaaaaagt
    22261 ctaaattaat atgtgacagc tttttaccta atatactttg ctttaatact acatattttt
    22321 tgattgttgt atactagtta taagattgaa tatttgtgtt gttgtagctg tcatattctc
    22381 actagcagtt gctttatcca ttgcttcttc tattgtcata gctctattta ctatagaaaa
    22441 ataagcatct ataccctctt cattacattt aactgcatct tctgttgtgg ctccagccaa
    22501 acctattact tttaccccat atttttttgc cagttttgca actcctattg gagccttgcc
    22561 catagcagtt tgattatcca atctaccttc accagttatg acaaaatctg catcttttaa
    22621 ctcttcctct aatttaattt cttctaatat tatttttatt ccagattcta attttgaatt
    22681 taaaaatgct aaaaaagcaa aacctaatcc tcctgctgct cctgctcctt ctacattagc
    22741 tacatcttta cttaagtaat ttttaactac tttggaaaaa tttctaagcc cattatctaa
    22801 ctcctcaatt atttctgatg ttgcaccttt ttgagaccca tatatgtatg cagctccatt
    22861 tttcccaaac agcggattat ttacatcaca ggctatttta aacttacatt catctaattc
    22921 ctttaatcta ttttctattt ttatatgtct tatttcattt aaaacttttc cacctaatcc
    22981 tactaacttg ttattttcat cataaaactc aaaccctaag gcttgaagca tacctatccc
    23041 agcatcatta gtagcacttc cacctattcc aactataaaa tttctatagc ctttatttat
    23101 tgcttccttt attatttccc caacaccata agttgtagtg tataaaggat ttctaagttc
    23161 agtcggaacc aatgttaatc ctgatgcttg agctatttct attattgctg tacttgtatt
    23221 ttttaataat ccatatgttg catccacttt tttagctatt ggtccagtaa cttctatagt
    23281 ctctttttta ccattcatcc cttgaactaa agcatctact gtcccttcac caccatcagc
    23341 aagtggcaat atctttactt gcgcatcttc tttaaccttc aatatcccct tttttattgc
    23401 atttcctgcc tctattgaag ataaacttcc tttcaaagaa tctatagaaa ttaatatttt
    23461 catatttatc tactcctttt tatttactct ttattttaaa tatataattt tataagctta
    23521 atttatcttt tattttatac tctatatttc actttgtata caacttatac ccttaatatt
    23581 cttttatctt ataaaaaagg gagtgtctca aaatgaactt tttagttcat gagacactct
    23641 ttttgtataa aattaaatat atgtttatta tttcaacaat gataaggagg attatctcta
    23701 ttttaagata attccttttt agcactatct aatcattttt agcactattt aaccaatata
    23761 gttttaactt ggttcattag gcactctaca tttacagagt tattatctat tgatatagat
    23821 tctaattctt tttcatttag gttagtccat ttagctgttt taatattttt gttaaatact
    23881 acgttagctg attttgtttc ttctttagaa ttgtaaaatc taactactaa tgcctcttct
    23941 ttttcagcct tttttacaac acttaacaca aggtccttat tagtttcctt aagtaaacta
    24001 tatgaataat ctacttctat ttctgattga ttcaatttca tagcattata tggtatttta
    24061 ttataagttt gtatttttgt taaatactct ttagccatat ttgcaacatt tgcttctagt
    24121 gtagattttt catgagtgac tattgctaag tctattttta tatctcctat catttgagaa
    24181 tctggagtag gcaactttat tcctgatgga cgacctgggc gtcttgccat ttcttcttta
    24241 cctaggaatc ctacacttct aaataatgtt atagctattg tgtcaaattt tttccctact
    24301 atttcaaact ctcttgtact gtttgtaaga acagctactc catgctcttt gtcagacaat
    24361 ccaacgaatg ttagcattgg atatatagaa tctggtcttt cactccattc ttcctgttgc
    24421 caaacttcca tagcatcatc atacacactt ctctttatat gtccaaactg attatcagac
    24481 acagaaaact ttgaagctat atttgttggt atataagttc taaccctaca atctcttgct
    24541 tggttattta tattgaatac tacatttatt atagctttat catttggaac atttataact
    24601 atatcaaagt caataaatga atctgctcta ttattttttc tagcttgtat atcttttggt
    24661 acttttaatc tataatttat ctttatctca ttattaaatc tatttttctt tatagtggaa
    24721 tttgctacta tattatcgtt aaatattaat ttctcatttg gaagtggtga aaaatcatac
    24781 tcatctcctt catctcctcc attttccatc aataatacat tttcaaaagt tttatttaat
    24841 tttttatcag atatctttaa tgtcccgttt tcattaactt gaatagtata aaagtcatta
    24901 tctaccatgt cacatacttc ataagctttt tctaacatat gttcaacttc ttttatcata
    24961 tacgccttat accccattgc aggtatttta tctttaaagt ttattgtata taccataaat
    25021 gaatcatagt ttccataatg aacaatttga cggtctatta atcctgcatc tattacttct
    25081 ttatgtacca cttcaaaatc caacttatta ttatctttat caaatatttc aaatgctttc
    25141 attttagtta taacttgggc gttcactatt tcattcctat tgtaaggcat taagttaaat
    25201 gctactaact tatctaaagc catttcacaa ctcattgcat caactattct tcttttgtaa
    25261 aatgttatta attggtctac tttttcctca gctaagaaaa atctattcat tatttcctgg
    25321 tgaactttat ctgaacaaca acatccaatg ctgtcatgag catgattttt cattatttct
    25381 ttccaaatta actctattaa tccatgatga tattcaaatc ctagagaata tgcaatagaa
    25441 gctagtggct ccaatatgtt ggttatcttt gattctattc tagcatttgc tgattttata
    25501 tccattcttg ttgagaatat acttctatga accctcatat atttcccatc taaaaattcc
    25561 ccttttattg tatctatatc cttatttttt tctagctctt taaatatgtt ttcatatcta
    25621 cttaagaaaa attttctctc tggataacat tcttttaatt tatatataac ttcaaatata
    25681 ttcttttgaa ttggcatttg gtcatgtcca tttggaagta ttatatggtc agtagttgca
    25741 cccttatcaa gaaccggtaa atatttatcc attcttgttc ttagctcatc ttcattcatt
    25801 ggtagatatt ttcctattgc ataacctaat ggaagtattt gaaccaaaac agaagaccca
    25861 tcatcactct tccaattaaa ctctgtttta ttagttcctt ttctttcaga acatcctctc
    25921 caaaacattg agtattttat atcaaaccca tttagtattt gcggcatttg agcactttga
    25981 ccaaatgaat ctggtaaata tcctattttc atatattctc caaattcatc acaatctttt
    26041 attccataca gtaaatttct aagtatggat tctccaccaa ctaccatttc atcagtttga
    26101 gtataccaag gacctattat tagccttcct tcttgaacta atttttttat tctctcctta
    26161 cattcaggct taactgctaa ataatcttct aatatagcag tttgaccatc catcacataa
    26221 tatggatagt tatgattatt ttctaacata tccattattt cttccatatt atttacaagt
    26281 aatattctag attcctcagt tgagaagtac cattctctat cccaatgcat atgaggaact
    26341 atgtgtacat tttttttcat tgcttatcct ccaaaactta tctgataata ttttctgtct
    26401 aattaaaaat ccaataaaaa tactactgag tttacacaat tttttaatat ttattttatc
    26461 actataatta aacctttgat ttaaatatct aataacgtat cttttaatgt atctttcttt
    26521 gatttttttg tttcctagtt attattaata atattgctga tattatagct ccaagtactg
    26581 ccgcaaagaa ccatattgat gctataataa atcctgacat acttccctct aaaagcgcca
    26641 atgagaatat ccccgctcca ggcacttgaa gacctatatt gaagaatgct actatagacc
    26701 cagttatggc tgaaccagct actaatgatg gtataacatg gattgggtcc tctatcataa
    26761 atggtattgc accttcagtt attccagcca atcctaatat ccaagtttgc tttcctattt
    26821 ccttttctga ttctgaaaat aaatctctgc ctaaaacagt agctattgta actccaaaag
    26881 ctgagaccat cttaactgat gcaaatattg cataaggtat aaaatttcca cttgctattg
    26941 ctcctattgc aaatgtatag gctgctttat ttattggtcc acctaagtca aatgaaacca
    27001 tagctcctat tattgcacct agtagtattg catttattcc agtcatccca tttaaccaat
    27061 ttataagacc attgttaagt gctgctacag gctttcctat tacaaataac atcaataaac
    27121 ctactaccaa agaaccaact actggatata cccaaaaact tacgaatcca gctaatgtgc
    27181 ctttggctgg tatattcttc tttaaaaatt ttactatata accagcaagt aaacctccta
    27241 acatacctcc aagaaatcca ctacctatta aatcagctgc tagacctgct gcaaatcctg
    27301 gtccaagacc tggtttttca gctagtgagt aagacatata tgctgataat actggaacca
    27361 tcaatgtacc taacatccct ccagaaagct ttcttaattg ccataaccaa gaaccttctt
    27421 gattgtatag ttcttgtaaa ccaaatgctt gagatgctaa tactgcaaat gcaagtatca
    27481 tacctcctgc cacaataagt ggtattatat gagatactcc tgtaagtatt gctgatttta
    27541 catctttttt ccatgatgat ttttcacaat atgatatatc agatgatttt tctatataat
    27601 cacctttacc ttgtttttgt gctacttcta aagcttcgta tattatttct tttgcccttt
    27661 ttagtggagc tgctactgac gtttttatgt ttggcaaatg ttcaaatctt tctgtatttt
    27721 taggtgctac atctgttgca tatattattg catctgcctt ttttattata tctggtgtat
    27781 gagggtccac cataccatta gctccttgct tttctacata cacatccaca cctatttctt
    27841 tgccagcttt aattaatgct tctgctgaca tatatgtatg tgctatacct gcagggcatg
    27901 ctgttattgc taatacaatt ttttttgatt cattttgaat cctaatctct tcttttattt
    27961 gttctttctt ttcttttata tctaagttat taaataactc tttcttagtt tttgaattta
    28021 acaacatatt catataatct tcattagaca gtctagtaac taactcagaa agtatttgta
    28081 tgtgtgtatt tccaccttca ctttctggta ctgctattaa aaatattagc tctactttat
    28141 tatttgagtc tacgctttcc caattttcta ttggtgtctc taaggtcata gctgcaaatg
    28201 cagcttcttt aactgactta gattttccat gaggaattgc aagccctctt tcaaaacctg
    28261 ttggagataa agtttctctg tacattacag cttctaaata ttcctcataa gatgttaact
    28321 tgccttcatc atatagcttt cttgctaaga actttattgc gtcttccttt gtttttattt
    28381 tttgttttaa aaaaacaagg ttttcatttg tgattttctt aaattccaaa attacgacct
    28441 ccttaaatca tttaatgtta tgaatatagc ataaaataaa tcatgaaaat gtttttttga
    28501 aattatacac acaatttctt cttaaaaaat aaggatttgt gtatttatac acaaatctct
    28561 atcttaacca gtattgttct attattgctc tcataatcat cataattgga accttatctg
    28621 atatattgta cccatttact tgtttaattg ttgaatagca atataaactt atatctgcaa
    28681 tttgttgcaa ttgattttca catatatgtg ttagagctac taccttacaa cccttattaa
    28741 ctgctgtctt tgctgcttct ataacttctt tcgtttctcc acttaaacta agcgcgaaaa
    28801 acatatcttg ttcacctaat tttcctatat catgaagtac ttcatgtctt tggctatagt
    28861 aagttatatt atcatgccca cctctaattt cttttactaa tatctcacat aaaggaatgt
    28921 tttgacctat tccataaact ataatactcc tagatttttg aattatatct gctactttat
    28981 ttattttttc ataatctatt agctctaatg tcttttttat atctgattca aaaccatttt
    29041 ttggctcttt atcctcattt tttaactctt cttttaaatt gttttttagt tgagagaaac
    29101 catcatactc cattttttta catagcctaa ctatggtatt tgggacagta aaaaatttat
    29161 ctgctaaaga ttgtattgat aattttacta tttcttcttt gttttttctc atatactcaa
    29221 ttatcatgtc ttctgtatct gtaaatttat actcatattt ttttgctctt tcataaaatt
    29281 ccatagtttc tcctccatct attttattat tcataagtaa aattcaaaaa tattagctaa
    29341 aattttcgtt aatataatta taatttaata tttttaattt attaatgcat actactcttt
    29401 ttattataca taaaaaatgt gtacttccta tattgataga gatgattcag gtttttattt
    29461 tgatacatct ctatcaatat ttttttagaa aatacacatt tttttcaaat acttattttt
    29521 aattaaaact ttaggtaatc tattctttat atataattat tagacttgaa aatatcttta
    29581 aaatcaacca attcaatatt atttcttttt aaccacattt taacttcctt atcacatagc
    29641 attgcatgtt catatactct ctcttgaata agagaagatg attttataag actataatct
    29701 ataaatcctg gatgagtaac catcaatgtt atatctttat ctaaatttat atatttttct
    29761 tcaaataatt taacaaaaga tttatttaac ttgtaaaaat cattctgagg atatttactt
    29821 ggcatgctaa tacatgaatc taaataatag ggacaacttg gtataccgta ttcttttgca
    29881 gcatcacaca ttgctgatat tgatacatct tcttctatac catgtatatc aatatactca
    29941 ggcaatttac tcatatacaa cttgtaagtt tcaatttgag cacaaacttc ttcatatact
    30001 tcttcataat taaaaacctc tttacctgtc agtagttgcg ttcttcttat tttgctagaa
    30061 acaaattttc caccttcatc taacaaattc tttatcttaa tatgttcttt acaacaaggt
    30121 tctcctatta ctaaattaac atgtaaacca aaacatacat catcattttt taccatttct
    30181 atagcttgtc ttgaaaaagg catattcacc atgattccag cacttgtaac aataccgttc
    30241 ttataagaag atattatccc tagtgtaaca ccttctgaat atcctatatc atctgcccta
    30301 actactaatt tcttcatttt atgcatctgc ctgctttctc aactcatcaa gttgcatttc
    30361 catctcatcc tttactttaa caacatccgt tcctatgatg atttgaactg atgtattact
    30421 caatttaatt acccctttgg ctcctgtttt tttaatatta tcatcattca aaatagtggc
    30481 atccttcact tctaaacgta acctcgtcat acaattatta agagtcacta tattttcata
    30541 accaccacaa tttttaatta tctgcttagc catatatgaa aaattaccat gagaaagttt
    30601 taaattcttt tctttttcac taactttatc acctatctct atctcaactt catgtcctgg
    30661 tgtttgtaca ttatctttta ttattatata tttaaatgtt acataatata atacaaagaa
    30721 tacaactcct acaggtataa ctaaccacgc tttatctcca taactaaaac ttaagacata
    30781 gtcaattata cttgctccat taaatgaacc caagcgagca cccagtaaat atagtacaac
    30841 tccacctaga ccagaataaa aagaatgaat tatatataac tttggagatg caaataaaaa
    30901 cgaaaattct attggttcag ttactgttgc cataaaagaa gtcaatgctc cagcggtcat
    30961 tgcacctttt acttcttgct tctttttatc ttgtgctgtt ttatatatag cataagaaat
    31021 agcaggtatt gaaaacatca taattatgta aaatcctgtc cagaagaatc ctgcatttgg
    31081 gtctcctgcc ataaatctag gaatttctcc tcttataact tctcctgttg atgttgtata
    31141 ttcacccatt tcaaataata tgtaagcatt taaaacttga tgtaacccag ttggtattag
    31201 tgcacggtta cagaataaaa acaagaatat tcctattgca ccagctcctc ccaaccaaaa
    31261 accaaattga ttcattgcag cttgtggata tatccataat aaactaaata tatatgataa
    31321 aattaaagca accactggtg atagtgtaat tacacatttt tctcctgcaa agaagcttaa
    31381 aaatgctgga actttaactt tatatgtttt attgtaagtc catgctatac aagctccaac
    31441 aattatccca ccaaatacac ctgcatttaa tgtatcattc aataactcta aatttgactt
    31501 aaatatcata tatgcaacta cggctgcaac cattacactt gtcttgtctt tagactttga
    31561 ataagcacta actgctccta ataaaaataa gtagtccaca tttgaaaata ctatattaga
    31621 tatattagat atagctggca cattaagcat atttggacca aataataatc ctaatatacc
    31681 tgctgctggc attacagcta ttggcaccag catagtcttt cctaaaagtg acagttgttg
    31741 catcattttg tttaagaacc tcttcatatc caatcccctt tctatttcgg tttaattttt
    31801 tgtgaaaacg atttattcac attttatatt ttaaatatta tatttgttta aacaaattgt
    31861 caatattttt tattcatttt atttttatca tttagtttca tatctaaatc cttttaagta
    31921 tattgctata tctagcctat tttttaaatt tatttttttt atttttaaaa tatgtttaac
    31981 catgtatttg aaacaaccca caaaatagtt caaatttatc ctttttatgg tttatataat
    32041 tctattttca ctatacatgt ctaaacacat ttgatataat ttgttcatat cagttcaata
    32101 atattactaa agacctttaa ggagggatag gggtatatgg ctaaaaataa attttcagag
    32161 atatatgaag tattgaaaga ggaaatactt gatggtaaat atacaagtaa tatgatgctg
    32221 cctactgaac ttcaacttat tgaaaggttt tcatgtagca gaaacactgt acgtagagca
    32281 atttctcaat taaacacaga aggatatgtt caaagtataa aaggcaaggg tgttgtagtg
    32341 ttagaaaata gttgttctaa tgattttttt ctaaatatgc acaatttcaa aggtgtagaa
    32401 tcgattgtag aagataaaaa ggttaatact gctactagtg ttctccactt cagcaaaatc
    32461 ttaattgata ataagttatc taaaaagaca ggttttaaag ttggtagtga agtatattac
    32521 cttcacagac taagatatat tgataacata cctaaaattc tcgacattaa ctattttcta
    32581 tgtagtatag ttaaagacct tgatgtttct atagcacaag gctctattta taaatatata
    32641 gaagagtgta taggtacaaa aattgtttca tccagaaaga tatttaaaat tgaaaaagct
    32701 acagagcttg agttaaaaac gctacctcta aatgattata attgtgttgg tgttattaaa
    32761 aatagtgtat atactgatga tggaaagctc tttgaatata cagaatcaaa gcatacacct
    32821 gaaacatttg tattcatgga tgttactcaa agatattaaa aaaattatgt ttaattaaac
    32881 ataatttttt tacgtttaga cactttatcc catactaacc tcactattct atatacaatt
    32941 atatgtttat acaaataatt atttattgta tattttagct ttaatattag tttaatactt
    33001 gacaatttgt ttaaacatat tataattaac ttataaatta caggaggtat caattatgaa
    33061 aaattggtgg aaaaaagcaa ctgtctatca aatatatcca aaaagtttca aagatagcaa
    33121 caatgatggt ataggtgata taaatggtat tatagaaaaa ttagattatc tgtattcttt
    33181 aggtgttgat ttattatggt tgactcctat gtacgtttct cctcaaagag ataatggata
    33241 tgatatagaa gattattata atatagaccc aaaatatggg acaatgagtg actttgaaaa
    33301 actcttaaaa gaagctcata aaagagacat aaaaataatg atggatatgg tcctaaacca
    33361 tacatctact gaacataaat ggtttaaaga atctaaaaaa agcaaagaca atccatatcg
    33421 tgattattat ttctggaaag atgctaaacc tgatggttct gttcctaata actggatatc
    33481 cagatttagt ggaactgctt ggaaatatga tgaaactact aaccaatatt atttacattt
    33541 atttgaagaa actcaagctg atttaaattt ggaaaatgaa aaggttcgtg aagaatgtta
    33601 caaagtacta gaattttggg ctgataaagg tattgatggt tttcgtttag atgtagtcaa
    33661 cctactctcc aaaacacctg gattacctga tgaccctata acagggccaa aaggagatgg
    33721 aagaactcat tacgctgatg gtcctcgtat acacgaatat ttacataata tgaatcaaaa
    33781 agttttcaaa cctaaaaata tagttactgt aggtgaaatg tcatctacta cacctgaaga
    33841 gtgtatcaac tatactagag aaaatcgaga agaactatct atggttttta gcttccatca
    33901 tatgaaaaca gattataaag gaggcgcaaa atggactaat gaaatgttct ctctggataa
    33961 attaaaaaaa gcacaatctg actttcaata taaaatgtat gaaggaaagg gatggaatgc
    34021 tctattttac tctaatcacg accaacctag agctttatct cgatttggag atgatagatt
    34081 ttttcgtcaa gaaagtgcta agatgttagc cataacacta tttggtctac aaggtactac
    34141 atacatatat caaggtgaag aaataggtat gacaaatgcc tactttacta aaatagatga
    34201 atatgatgat gctgaaagta agaatgcata ctatcatatg attaaatctg gtattgatga
    34261 aaaggaggca ttgcttatac ttcaacaaaa atccagagat aatgcccgta ctcctatgca
    34321 atgggataat actatttata aggggttttc taatcataaa ccgtggttaa aagttaataa
    34381 tgacaatatt agtgtagaaa atgagcttaa tgattctcgt tcagtatttt atacttatca
    34441 aagattaatc aaatatcgta aacaatatga tatttttaca gaaggtactt atagattgtt
    34501 agatgaaaat cataaaaatc tatttgttta tgaaagagag tataaaaacc aaaatcttct
    34561 tgttataagt aattttactc atgataatgt agtatataaa ttacctgaaa cttatttgaa
    34621 taaattaaac tacacaatac ttgaaaccaa ttacactaga gatataattg aaaataaaat
    34681 ggaaataaaa ccttacgaaa gtataatgat atattataaa taaagttatt tatataaaaa
    34741 ttggtataac accagggtgt tataccaatt ttactaaatt caaaataaca tttatagaat
    34801 atctatagat tacaataatt atttgacttc tacatcatta tatttctttg aatatacttt
    34861 agtcatatag aaatatatag gaactacaat tatcatccca cctaatcccc aaactatctt
    34921 cataggttca tcaatacctg cttttatcag taataatata cttataataa ctgcaaatat
    34981 tggtataatt gctcctccag gtattctaaa atttacatct ttatcagcat acttctttct
    35041 caatattagt acagctaaac atgtaggaat atattgaata aatctagata caacacttat
    35101 ttgagataat gttgtgaatg aacccgacca tgctattagt aaagttccta tgacagatac
    35161 tataattgct atataaggag ctccattttt attagttttt cttatagact taggcattaa
    35221 tccttgttca accaaagcag cagcagacct tggtgtaaat atggatgatg caatatttat
    35281 acctccaaca gatactatag ttcctattgt aattatatac ccacctatag gacctagcat
    35341 cttaatggca gcatctgcaa caggagtagc tgaccctgcc aagccattac ccaacacccc
    35401 tatacttatt cccagtataa gcatatatat tactgatact acaaacatta ccattaccaa
    35461 tgctttaggt acatttttct tagggttctc catatctttt gctgcaacag ctaaagattc
    35521 aaatcctgta aaagcataaa ataaagttat agatactgct acaaaagctg atgacgcact
    35581 ttgacccttt tgtattatta caaatggggt aaaattagaa ccctttataa agaataatcc
    35641 tacagctata aataaaacta ttggtaataa ttttcctata gtcatgatat tatttaagtg
    35701 cttagattgt ttcattccca taaaatttat aatcccaagt cctacaacta atataccaat
    35761 tattatattt ttaataagtg gttctgctgc ttgtggccat acagatgata gtgttacagc
    35821 aaaaaaattt gccatagtag cccaagcaat catactaact atccacttca taaatccaac
    35881 ttcaaatcca acaaaatctc caaatgcctc ttttgcataa agataaggcc ctccatcagt
    35941 atcaaaccaa ctacctgctt cagcaaaaca taaagcaata gaaacagcta aaagcataca
    36001 aaaaactaat actaatatac ttgctggacc tatatctgta taagcatcac caggtaaccc
    36061 gaatatacct gaccctatta ttgcattgat tcctaatagt acaatactcc aaaaacctaa
    36121 cttaatgcct ttttccattg ttattccccc caaagccaac ttaatatatt tatatttttt
    36181 atttagatgc ttctttaact attgccataa atattttttt catattatca taatctctag
    36241 tcatcatttc tggatgccat tgtatcccta ttacaaactc acttccttcc ttttctatag
    36301 cttcaattaa accgtcttta gatgtagctg aaacaatata tcctggtgct actttgttga
    36361 caactaagtg atgaaaacta ttaactaaaa cttctttttc tcctaatatt tcatataatt
    36421 ttgtcccctc ctttatctgt actgtatgtg taggtacatt agatagatgt tgctgattgt
    36481 gtttaatata agcaccttca attaaagata aatcttgata caaactacct ccttcagtta
    36541 catttataat ttgttctcct ctacatatac ctaaaactgg ttttttcata tctaaagcat
    36601 gttttaataa tttcatatca aaactatctc tctttggtga tattgccccc aacttattat
    36661 gaggttcttc tttccatatg agtggattta catcttgtcc cccagatagt ataagcgcat
    36721 ctatattaga tacctgctcc tttataatct catcatcata tacaatcggt acaatatatg
    36781 gtatagcttt acacataact actgattgta tataatcatt atttacataa gccctttcat
    36841 agccaggaaa cattcctcct tcatcaatta gtaaattacc tgatattcct attattggat
    36901 atttcttttt aaccaatttc ttcacccctt aacttatttg tgaacaataa aaaatgctac
    36961 ttctcttaaa gagcaagtag cataccaaga ttttatttat ttgaacatta taattcattt
    37021 tagaaatact ttttttgaat acaagtaata taaataactt tatttactta tattttattt
    37081 taatcctgtt tagtttgact tttttaaatt gttttaagta caactaatag gtaaaatagg
    37141 catttagatt ttccttttta tcaacctatt aaatactgtt taatatattt tttccccttc
    37201 tatttaattc acttcccttt ctaccagatt cacatattat caaatccagc tcttttaaca
    37261 tattaagtat tccccttacc tcaccttctg ttatactgta gcctaattca tttattgaat
    37321 taacaatacc atttctacct atatttttat taagattatt ggcatttttt aaacactcca
    37381 ttactaactt tactttctct atagatgttc tatcctttaa tatgcttatt tctttactaa
    37441 aatcttcagt tatatttaat aagttatgag gtaaatcatt tattaaaata acatcctcag
    37501 aactcataat gttaatatac atagcagtat tttttaattc tcttatatta cctggccatt
    37561 catatgcatt aataatgtct tgaacacctt gactaattgc tctcttcctt ttcataaaat
    37621 gctttaacat aacttctata tcttcttttc gctctcttaa aggtggtata tttatgggca
    37681 atacatttat cctatagtat aaatctgctc taaatctatc tccttttatc atttctaata
    37741 aatttttatt tgtagccgca attactctaa catctatatc tattacattt tctcctccaa
    37801 ccctcattac ttgattctct tgtaatactc taagtaattt gctttgtaag tatataggca
    37861 tgtctcctat ttcatccaaa aatatagttc cattgttagc ctgttcaaat aatccttttt
    37921 tacctccttt taaagctcct gtaaaagcac cttcttcata tccaaaaagt tcactttcaa
    37981 gtaaattttc tggcatagct gcacagttta tcgctataaa tgactgacta tgtcttggtg
    38041 atgcattatg aatagactga gccataagtt cttttcctgt tccactttct cccgttataa
    38101 gaattgtata atcagattca gctattttca tacctaattt cttagttctt tccatcttcg
    38161 ttgaaactgt ttttatatca tcaaaagtat atctagcaac ttgtcccttt tctctaagct
    38221 tctttgatag gttttgctcc aattttctta tataagttat ttcttgaata caaaaataat
    38281 acccagtaac ccttccaaaa ctctctatat tatacttatt tacgtttaag tacttattat
    38341 taaattttat aacttcatca tgtgccttac tcaaactaaa aatcttttta atattctcat
    38401 tctctaattc atctatattt ttccctataa tattactttg aatatctagt atatccctta
    38461 aactattatt gcaaataatt atgtttccct tatctgatac cattgccata cctaagtttg
    38521 ataaatttat tatagaattt aattcatctg cttttatagt aagttctttg tatttagtat
    38581 ttattcctga atataaacta attatttcat ctgtatactt aattagcctc tcagtaattt
    38641 ctttatcatc cagtttgagt tttgctatta tttgaataaa cgttgaacta tcaatatacc
    38701 tatggcctaa atcaataact tcttctatat attttggaac taaacttgac tctcctggcg
    38761 ttatagctat cttaatattt ctatattctt cattgggatt gtatggtata aaattcaaat
    38821 ggtttattcc tatattgtat aaattagata ttgtttccaa tacagtatgc ttattatcgt
    38881 ttactactaa tacatctatt tcttctggca atgaaaataa cttatatata tccttttgct
    38941 ttatgctcct atttatagtt attatctttg agacatcttc tacatatttt tttattttta
    39001 atactctatc atctatcatt accaatatta tgtcatcttt tattaattcc ttctctgtta
    39061 ttttatttaa ataataattt tttatcatta ctttatcttt aaatatcatt tctagatttt
    39121 tatttaagaa acattctaat ttagacaacc catcagttac tactgctatt gttttcattt
    39181 gattactcct tttaaaatat actctactct tttataatat acatctgatt ttcactttaa
    39241 tagcacaaaa aattgtacat ttagtatact aacatattcc tttattagtt ttatgtccct
    39301 aaatatcata gttgcaatat ctatcatatt tcttaaactt ctataaagca aaaattgtgc
    39361 caaattaata attttcttaa ttttattcta aagttcctca atagtattta cagacttagc
    39421 acttatagct tataagtctt tatattatta atctattttt ataggttaat tgtacaaatc
    39481 atatatcact tttacctatt aaatgcatta taaaaaagat ggaataacga ttcgtcactc
    39541 cacctttttt gtaataaaaa attgtatata taaacactat ttactactct tctaagtaca
    39601 aacagtatct acatactata acctttcacc attagattct attacttgtt tataccagtc
    39661 aaaactcttt ttcttaatcc tagacaagtc tccacttcca tcatcatact tatccacgta
    39721 tataaaccca tatctcttgg tcatctcacc agtagatgca cttactaaat ctatacatcc
    39781 ccatggagta tatccaaata aatcaacacc atcttctaca gcttccttca tttgctctat
    39841 atgtgctttt aaatactcta tacgatagtc atcatttatc actccattag attctttttt
    39901 atcataagct cctaaaccat tttcaactac catcataggt attctatatc tatcataaat
    39961 ttcatttagt gcatatctaa gcccttcagg gtctatttgc catccccatt cactagactt
    40021 aagatatgga tttttcactc cacctaatat gttgccactt gtattatctt ctttagaatc
    40081 actacttaca catgtagaca tataataact aaatgtataa aaatctacac aaccttcttt
    40141 taaaatttct aaatctcctt tttccatttt tatttcaata ttattttctc caaaatatct
    40201 attcatataa gttggatatt cgccacgcac ttgtacatct ccacagaaat aattcattac
    40261 tctactatct ctttgagttt taactgcatc atctggatta caagtataag gatacattgt
    40321 tgcaaatatt tgcatacatc caacttgata atttgaatct atttcatgag ctaatttaac
    40381 agccttagca cttgctacaa attgatgatg taacccttga aaacgaagtt gaggtatatc
    40441 tacttggttt ataaaatctg tagtcccttc atttaatatt ccttgtgata agtagcctcc
    40501 cataggcatt gtagctgaat ttatttcatt aaatgtcagc caatatttta cttttccttt
    40561 atatctttta aatattgttt cacagtaatt catatacaag tctattacct ctcttgaaac
    40621 ccatccattg tatttctctg ttattgcaaa tggtatttca tagtgagata ttgttattaa
    40681 tggttctata ttatatttta aacattcatc tatcacattt tcataaaatt taagtcctgc
    40741 ttcatttggt tctttctcca ttccagttgg aaatattcta gtccatgcta ttgaaaatct
    40801 aaatacctta aatcccattt ctgcaaataa agctatatct tctttgtatc tgtgataaaa
    40861 atctattgct tcatgacttg gatagaatgt tcctttttct ataactctcg ttattctttt
    40921 acttactttt tgagaacccc cagtacacat atctgaacaa gaaggacctt tcccatctaa
    40981 attccatgct ccttcacatt gatttgctgc tgttgctcca ccccataaaa aatcttttct
    41041 aagtaccatt actctacctc caataattta tttatagtaa atttatttta aaataaaaat
    41101 acatatatct aatttttaat taataaaact ctcttttaag ctaaaacagt tatcaattcc
    41161 tgactattat ttacatgctt ttgtgtattt tctataatat ccacataatc agaataatta
    41221 gtaacaacta ctggtgtttc taagcaatat ccttcttttg atatgctttc tatatcaaaa
    41281 gttattaatg tctgaccttt tttaatctta tccccttgat taacaaaaga tttgaaatgt
    41341 ttaccttcca actgaatagt atttaaccct atatgtatca atagttcaca accattatca
    41401 gatataattc ctattgcatg ttttgttgga aataaagtca ttatagtccc atcaaatggt
    41461 gccactactt ttccttcttt aggtgttatt gctacacctt ttcccaatat acctgttgaa
    41521 aacgctgcat cttgtatctg ctccaaagcc tttacttcac cttttattgg gctatatact
    41581 acttctcttt tgtttttttc tacaacgcct tgcccattat tatctttaga aatttctact
    41641 atagaatcat ctttccaaaa aaataatgtt aaaacaaatc ctactacaat agctactatt
    41701 attgctatta tagttgcata aacacctttc atatctccag ttgttggatt tatataatta
    41761 ggtataccaa atattccaag tccacccatt acataacttt ttacattcat tatccccatt
    41821 acagctcctg ccacagcacc acctacacaa ctatatatga atggtgtttt tcttggtaaa
    41881 gttattccat atattgcagg ctctattaca ccacatatcc ctgaaataac tgcaggtata
    41941 cacagctctt ttatcttctt atcttttaat ttaaagtaca ttgctgctac tactgatgtt
    42001 tgtgcaaaag atggtacaaa tgctcccact aatattgtgt caaaccccat agtcataagg
    42061 tttgacatag ctattggtac taatccccaa tgtagaccaa atattacaag cacttgccac
    42121 aataaaccag ttaatacacc acataaaatt ggtgaaaagt taaataaagc taaaaatcca
    42181 tttccaacta tattagaagc ataggtagct ataggcccta taaccataaa tcccaatggt
    42241 aaagaaacta ataaaactgc cattggtaca aagaaattct ttactacatc tggtattatt
    42301 ttagcaaaca ctttctccaa cttagctgct aataatacta ttataattac tggtactact
    42361 gttgaagtgt agttcattgc tattactggt aatcctaaga aagttatata tactggtgat
    42421 tcaaatattg ttccagtaaa tagattgtat aaaggttgac ctatagatgc tagagtatct
    42481 aattgaatac ctgaatagca taaaactgca cctataagca ttccaataaa tggttttaat
    42541 ttgaatttct tagcagctgt atatccaaga attattggca tatactggaa cattgcatct
    42601 ccgattgcat ttaacataat atatgcacca tcagtttctc catacaaacc caatgctgta
    42661 aatactgcat taaatccttt tatcatacca gcagcacaca gaactcctaa tattggttga
    42721 aaaactcctg atattatgtc tataaactta ttaagtaatc cttttggcgc atctgaatct
    42781 gaattatcta ttgtatctcc tcctaatcca gccaaatcgc acacaacttc ataaacatct
    42841 ggaacatgat taccaataac tacttggtat tgacctgcac ttttcattac agtaactata
    42901 ccatccattt tttttagcac atcatcattt gctattgatt catcttttaa tttaaaacgt
    42961 agcctcgtta tacagtgaga taaactgcta atattatcct tccctcctac attctcaatg
    43021 atttcttttg atagttgttc atatcttctc attttttctc ctccattttc tttttgaaat
    43081 atgaaggttt ggccaactta atgtcaccac ccaatatata taaacctaat tattaggatt
    43141 tatatctgac ttaatacttt tacttaccac tctttcaacg tgaatagtta agtataattt
    43201 ttcctcatct gttaaatcgt agttgtattt tttatgtata aattcatcca acttttctat
    43261 gcatttataa gcatttttat atttaacttt tattacattt agtaaatcat cttcattatt
    43321 ttcatgtaaa ttattactaa caattcgttc tgcaaaatac tttaagtgag taataaatct
    43381 atgataataa actgaatctt catcaaatac tattctgtaa tggtatttaa ccatatttga
    43441 aatttcttgt attatttgtg taatctcata tatagttttt atactttgat ccatttgtgc
    43501 attaactata tgcagtgcta taaatccagc ttcatcttct gatagtctaa tttcaaattt
    43561 tttttctata taatccaatg cttctaaccc tattttaaat tcactcttat aaaatctttt
    43621 tatatcccaa agtaatacat tttttacact tactccttca cgagctcttt ctattgcagt
    43681 atacatatgg tctgttaaag aaatataaat actatcattt aatcttttcc ctagtttttc
    43741 tttagcatat aaaattattt catctgataa ttccatatat cttataggaa tatctgctat
    43801 tagttcttta aatttatcag atatatttgg gtctgaaagt ttgaatatct tatcaatttt
    43861 atcctttgat atatgctctc ctgtacgttt tttataagct aaacctctcc ccataactac
    43921 tatttcctta tgattttcat cagttgtaat aattacatta ttattgagta ttttttcaat
    43981 aatcattgtg atgccccctt tattagcatt aagttaaaat aaatcttttt taattattta
    44041 gaatatttaa aatgtaattt attacataaa aaaaaaccta tttattacac attaaataga
    44101 ctcccatgag tcctgagtaa taaataggtt tagcttgtat ttaaacaatc actatccgtt
    44161 tgtattttat tttaattatt atttattata tgttagcaaa tttatttttt agtgtcaact
    44221 ttttctcaat attttaattc taaataaatt atataaattg catctgcaaa gtactatttt
    44281 tataaataaa caaaataaaa aaggtattac ttgctttaag taatacccat ttatttttaa
    44341 tttaagtggt ttttcttaat ctcatcaagt agtccatctt ctaaataaat ttctaatgca
    44401 catcctacca ttgcctttac tgctttgtga agtttatcat aagcttcctc agaatttgca
    44461 taatttagaa attctttttc atgaacatat accattggac ttatatctaa ggctatttct
    44521 gtatacatag tagggcaaac ttgacttaca ttacctatat ctgtagaacc tactggacca
    44581 tctttacctt ctaatatatc tgttatacct gactcaatca aattattctt tgttatcttt
    44641 tgaagtactt tgttatttat tatgttgtca aaagaatttt caaagtatct ataagaaagc
    44701 ttagctcctg tcattaactc tgcacctttt gcacagttta taactttttt agtcacttca
    44761 tctaaataac ttctttcagc agctcttaca aaaaacttac aagctgcttt ttctggaact
    44821 atatttggag cctctcctcc atttgaaact attccatgta ttctaacatc tgattttaca
    44881 tgttgtctta gtgcatttat cccagcaaaa gtaagttgta ctgcatctag tgcattaact
    44941 cccatatgag ggtgactagc tgcatgtgct gatacccctt caaaactaaa ctctaatgaa
    45001 tctattgcca aagccttaca gtttatatta ttaaaagctt ctatatgcat ttgataactt
    45061 acatcaacat catcaaaagc tcctgcctta actaaatcac attttcctcc tgttgtttct
    45121 tcagctggtg ttcctattag tacaattttt cctttaaaat tttcttttaa cttacttaat
    45181 actatacaag ctcctagtgt agatgctgca atccagttat gcccacaagc atgagctggt
    45241 tctttattgt ctccatatcc aggaagtgca tcatactcag ctaaaaatgc tatggtaggt
    45301 ccatcattat ctcctaattc agctctaaaa gcagtctcca tattacaata tggataaact
    45361 gtatcaaatc catattcctt cattttctct actaagtatt ttgatgatat gtattcttct
    45421 tcccctaatt ctggattgtt aaatataaaa ttacaaactt cttcaatttc ttttccaagt
    45481 tcattctcta tctcataagc tctcttcttt atactctcta tcataactta cctcccacta
    45541 gaatcctaga tataatccta ttgtcaaaaa taccattaca agaattgtta atactaccat
    45601 taaaggaatt acgaatctaa cccactttga ccaaggaata ttggctactg ctagtgctcc
    45661 cattaaaaca cctgatgtag gtgttattaa gttagttaca cctataccaa attgataagc
    45721 tgtaactatc atttgtcttg atacatctac taagtcgcca agtggtgcaa gtacaggtat
    45781 tgtaagtgat gcaagccctg atgctgatgg tattaaaaat gctattaatc cttctattaa
    45841 gaaagttaaa tttataaata tagtttttgg taatccattt aataaatttg ctgcagaatt
    45901 taaaattgta tctattataa atccattttc tgctattatt acgattccac gtgcaaatgc
    45961 tatacataac cctgctgtaa ttaaatctcc tgctccactt ataaatgaat taactatctc
    46021 atcttgttta agccctgatg ctattcctga tattatacct atagctgtaa atatcattgc
    46081 aatttcagtt atgtaccact ctagtcttaa aactccatat atcataatcg ccatacctat
    46141 tgtgaaaatg gctattacca tggcttctct ttttgtaaat tcctttatat ttgtatcact
    46201 attaaccatt gattcttgat ttagtgatat atcgtgtact aaagattttt tagggtcttt
    46261 tttaacttta ttggcataca tcataacaaa tcctatagat atagccatat atattataaa
    46321 cataacaact ctaaactcta ttccagaacc tggtactatt tgtgataatg cttgtgctat
    46381 tcctacagag aaaggattcg tagtagctgc tccaaatcca gctgttgctc ctataaatac
    46441 tgtggccact gctgtcaatg aatcatatcc catactggtc ataagtggaa taaatatcat
    46501 ataaaaaggt agtgtctctt cactcattcc aaacgtcgtc ccacctagtc caaatagaat
    46561 cattgaaatc ggtattatta aaaattgaaa cttcttcatt ttatttacag tatgagctat
    46621 aacagcttct atagcccctg ttttattaac tatcccaaaa gctcctccaa ctattaaaac
    46681 aaatccaact acttcagctg catctataaa tccgtttata ggtgctgtaa atacatcagt
    46741 aattccttgt ggatttgatg gtgctttttc ataagttcca gctacaacaa ctgaccttcc
    46801 gtctatatct actctttcaa aatttccact tggcactatc caagtaagaa ttgccattaa
    46861 cactattagc gaaaatatta ttgtaagtgt ctgtggcata gtaaactttt tcttttttaa
    46921 actgctcatt tttaatctcc ccacttttta aattaaatat tttattattg catcccttta
    46981 tcctattttg tatagatgta aaaaagctca acatggatac atgttgagct aaatgtgcta
    47041 aatacaacat acctgcatct tccctttatc ttgtttaaga tagctctcca tcaaaattcg
    47101 ggataatttt gatgacagta ctgtacctat taaatacagt cccagcacta gctttggaca
    47161 ctagcacttc ggcacttact ccttttatat tgcctcattg gtttccaacc tcttcaatat
    47221 taatcttagt aaatgcaacc tctacctcaa tctgttcgtt agatgaggta attcttatta
    47281 atttttattg attcaaagta attgtagcat aatatgtcga gattgtaaat attttcagta
    47341 taataattct tactttatga tgtcattcca tttttcacaa ataatttttt ctccatataa
    47401 tttaaatact gtagtatatt ttaaatacac acaaactttc cacaaatttt taattattat
    47461 aataccttta taataaaaaa taaaagccat gtaaacttat caatttacac agctttatat
    47521 acattaatct actccaagta cagctttaag ctttgatgct attgtaataa ctcgtggacc
    47581 aaatataact tgaactgagt ttttcttttt aacaactcca cttgcaccca attcatttac
    47641 ccagaaacca tcttcataga atccatcaat tgaatttagc tctaccctaa gtctagttgc
    47701 acagtttgta acatttttta tattattact tcctcctaaa ccttctatta tagaatgtac
    47761 aagctttgta tctttatcat ctacttcctc ttctactgca ttgttgtatg ctgttttacc
    47821 agcatctaca gattgtcttt ctctatattc tttcttacta taaagcttaa cttcttctga
    47881 acttcttcct ggtgttttta tatcaaactt cttaattgcc catgtaaata ttatataagt
    47941 aactataaaa aatattggta tcaaaaataa taatggtaac gcatccactt tttgtggttg
    48001 taataaatta ggaaacatat ttctaaatga ttctcccatt atagaaacat taaacatttc
    48061 tgtaaataca tacgtcaatc ctgccagtgg tacatatgct acaaaataca ataatggtgc
    48121 aacaaatagg aatgtatact ctattggttc cgttacccct accaacatgg ctgttactat
    48181 tgctggtatc aatatactta ttgctttttt cttattagca ttatcagcca acttatacat
    48241 agctaaagct gctgctggta atattgcaaa atgtattaat aaacgacctg ttgtgaagtt
    48301 acgagttata taagatagtg tatctgggtc tcccatttga gctaatctta tattattaac
    48361 accctcgtaa attgctccac ctacttccat tgttccacct actcttgtat attctatagg
    48421 gaatgcaatt aagtgatgta taccaaatgg taataatgct ttatccattg ctccaaaagt
    48481 aaatgttcct aataatccac tagaacctat ccaattacca agtttagata atgccaaccc
    48541 tactggtggc catataaagt acattgctgt acccaatact attgatgcta taaacatcat
    48601 aatcattgaa aatcttggac ctgagaaaaa agataatgca ctaggaagtt ctatgtcaac
    48661 aaatttgtta tgtactgcac tagctacaaa tccagctatt atagctccaa atatactaca
    48721 atcatatgaa aagattccta agaaagaacc atataaagaa cttcgactat atgcttctgc
    48781 ctctcctaat ccagatgcca taaacgcttc tgctgtaact gtatctgaat ttataccatt
    48841 tgatgctaac aatgtactta taactgtgtg cattcctata aataaaacaa ttcctcctaa
    48901 tgcaccccat cctttttcct ttttagataa tccaaaagct atacctgtaa caaagaatat
    48961 tggtaagtat ctcattacca taaatcctaa atcgtttatt actttaaaga atacatttat
    49021 aaatgaccct tcttttatta gtccgctaag ccctaatgca gaaacgtttt ccatatttgc
    49081 aaaagctgac cctaatccta taaatactcc tgctacaaca agtaatataa caggtatcat
    49141 cattgctcca gaaagtactt gtaatttatc cttcatagta ttatttcctc catttttatt
    49201 gaaatgccag gctatataat gcatatatat atacttctat tgctttgtat aatgtatcta
    49261 tacttatatt ttcatttgcc tgatgtgaat ttggattatc tccctctatt gtgggtccaa
    49321 atgcaactcc attttttaac actcttgcat aagatgcagc tccttttgta aaaagttcgg
    49381 cttcttttcc aaaaccttgt ttatatgcac ttgataattt atttattaat tcactatctg
    49441 gttttacata cagtaatttt aaatccttat atatatcaac tattaaattt tcttttttcc
    49501 caatttcatt aattctatta gttataaact cccaacttat acccttagga tatctaaagt
    49561 ctattttcat attcaattca tttttctcta agaagatact cattatacat attgtagaaa
    49621 ctcccatatc tacatcttct ttatatatcc ctaatttttt tccatgatta tcatctgtaa
    49681 agtatgaatt taaaatatcc cttattataa cgcctttatc atttagcttt ttaattttct
    49741 ctaaatcttt tgctaaacta aatgcacagt tatatgacct gtgtggattt ctcgataaag
    49801 ccctttctcc tgtatatctt actaatactt taccttcttc tagttcttct atctctgtat
    49861 aataagctaa tgattctact aaatctttct tatcatcagt tttaaaaaca gcctcaattt
    49921 tgtcacatac aaatccatct tctttgtttg actttatatc aactaaattg tgatttctaa
    49981 atttatcttt atctattttc tttcttagat tgaagtataa aataccattt tctccattta
    50041 ctattgggaa gtttccatct ggagaaaatg caaattttgg ctgttcattg acactaaagt
    50101 aatgctccat acattcccaa gtcgtctctt cagctccacc cactatcaat ctaatctttc
    50161 tcttcggctt ttcattaagt tctcttaaga aaagtaatgc atataaaatc cctatcaaag
    50221 gtcctttgtt atcattaact cctctaccat aaagatagtt atctttttga catactttaa
    50281 aaggtttact cttccaaagt tctttattat atattggcac cacatctata tgattaagca
    50341 cccctactac ttcttctcct tctccatatt ctatatgaat tgcatatcca tcaaagtctt
    50401 ttacaacaaa atctttgtct tttgctattt gaataagctt atcgaaggca tttcttattt
    50461 ctatcccaaa tggttgattt atatctgttg tactttcatc tctaattgaa ggtatttcaa
    50521 ctaaacttct aatatccttt atgatatttt cttgattgga ttttaaataa tcaataatta
    50581 gttctttgtt tactatcatc ttaattattt atcacccctt tatattaatt ttacaaagca
    50641 tttcagttca tatctacatt ataaattttg aatattattt tttcaatatt tcaaatactt
    50701 ctctaaatta ccataatgat acggtaaaca atcaacagat tatgacaaaa tcatactata
    50761 cctattacta atattttaac ttatctatta cgtctttttt aatgtaattt tattccaaaa
    50821 atagatataa aaagatgtag ccttagctac atctttttat atctattcaa cttccattat
    50881 tatgctatta atatctacat ttccaggttc tatcctatct tttactgttc tcttaacaga
    50941 atcagcatca tccattataa ctacaggagt tattatattt aaatcatttg acgttataaa
    51001 atctaaatct acttccatta ttaaatcttt agcttttacc ttatctcctg cctttacctt
    51061 aacatcaaac ggctttcctt ctagtgacat tgtctctaaa cctacatgta ttaatatatt
    51121 tgtattatca ttactcttaa tcattattgc atgctttgta tcaaatacat tgactatttc
    51181 accatcaaca ggagaatata acttaccttc atttggtatt attgctactc catctcccat
    51241 catcttttgg gcaaaaactg catctggaac atcttctaag ttcactaaat ttcccttaaa
    51301 agggctatat attttctcaa tcttattttc ttttttctta aatatattaa acataacaca
    51361 ctctccatat tataaaattt tatgataata tttttcttaa agcttttgct acaccatcat
    51421 ttacattagt atcagttaca aaatctgcca tagattttac ttcatctgta gcatttccca
    51481 tagcaatacc aagacctgca aatttaatca tggatatatc attttcatta tcacctatac
    51541 aaataacttc tttgctatct atacctaata tatcacaaaa ttttttaact gcaatacctt
    51601 ttgaagtacc tttactcata acttcaaaat ttatctttcc agaacttact acttctaaat
    51661 cttcttgttt tttaaattct tcctttgctt ctctcaaaga atctacgttt tctgagaaaa
    51721 gtatacattt taaaatttca ttatcagctt tttctaataa agtttcacaa cttgcatttt
    51781 ctattatttc aattctgtct tcttctttat ttttactatt tattaatcta tatggatgtt
    51841 cttcacctat ttcattgttg gatataacta ttttacagcc tttaaaatgt ttgtttaaat
    51901 tatacttttc acctattgta tagattttct ttatagcttc tttgctcaaa accttcttat
    51961 actcatatcc cattaattta aaatatgtcc cattagttga tataatatat atatcatccc
    52021 ctaaaacact tgtaaaatat ttcatagcat tataaggtct tcccgtaact aaggctacct
    52081 tgataccctt ttccatagct tcttttagtg ctttaatatt ttcttctgat acttcaaatc
    52141 ctttccccat caaagtacca tccatatcag tacaaattaa tttatatcgc atttgtatca
    52201 catcctagta agatttttct aatatttcaa caaggtcttc tttctttcca aaaggtaaaa
    52261 atcctaaaac tggacagctt gttatttgtt ctaattgttc ctttgttaca ttaaactcag
    52321 acatttttgt cttaagtccc actctcttta aaaaactttc tatttcattt cctaaagttt
    52381 tagctgcttc atcatcttcc actttttcta attcttggtt tagcattctc gctactttgg
    52441 caaatttctc tatattatcc ttatagaact tttctgcaaa ttttgggaac actatagcca
    52501 aagcctcacc atgaggaata tgtgtaactc ctcctattat ttcacttagt ggatgtggtg
    52561 ctgctgctcc agcatttgcc agtgaacttc ctcctaatgt atctgctact gctaactttg
    52621 ttctgtattc tatattattt ttatcttcca ttactttagg tagatagtta atgactaatt
    52681 ccatagattt ttcagacatt atctctgtaa agtcattagc atttggattt atataacttt
    52741 caaatgcatg agtaaaagca tcaaatgctg ttgatgcagt aattctctct ggcagagtta
    52801 acataagctc tgggtcaact atacattctt ttgagaaaca attttgatga aatattgtat
    52861 ttttctcgct tcctctcgat attactgctg cttgtgtaac ttggcttcct gttccagaag
    52921 tagttgatac agctattaat ggtaattctt tttcacttac cttatcgtat attttaaatg
    52981 gatttgtata tgtactaaac atataatccc aatctatttt ttctaaacca aaagtcaatg
    53041 ctaatacttt tgcagtatct atacttgaac ctcctccaac agctaaaaca acatctacag
    53101 attctttttt agctaattcg aacccttcat ttattaattc tacagttggg tttggctcta
    53161 ctctatcaaa gtgtacaact tctatacctt tttctattaa attcaatgta actcttttat
    53221 ataacttgtc taatggctct acatttggag tagtaactaa caaacatttc tttccatatt
    53281 ttcctattat tctaggtagt tcacttaaag ttccttgtcc aaaatttatt cttgatggtt
    53341 gataaaagtt aatcatattt ttcctccctt aaattattga cttcatattc aaattataca
    53401 tatcatagga tttttaaggc aataccttca ctgttaactc ttatttacta aagcttatgg
    53461 caattattag acaataaatg ttttattatt accatatcat tatgtaaatt ttattaagta
    53521 cccatgttat aattttataa tcaatcttat tgtataattg acttaataat aaaaatagag
    53581 tatatacact tttttatgac aaatagctta aaggagaatt aaattatgaa atatttgata
    53641 gacagtgcta atcttgatga aattcaaaag tgtttaaagt atggatttaa gggaataaca
    53701 gctaatccat ctatgtatct taaagagaag gtaaacttcc atgaatttat tgagacatgt
    53761 actaatttaa atccagaaat attaactgct gaagtcattg gaaataattt atacgaatta
    53821 attaattcat gtgataagat attaaatata aataaaaaca ctataataaa aataaatttc
    53881 tctgaagttg gcttagaact tataaacata ttaaatagta aaggtgtaaa aactgcatgt
    53941 accttgattt tcaatgtaaa tcaagcatca ttagctataa atgcaggtgc tgattaccta
    54001 ttccctttta taggtagaaa tgatgaaaat gggaatgatg gtataaaaaa ccttacagaa
    54061 atatgtaacc ttatatcaat aaatggatat aatactaaag ttgttgcagc ttctattaaa
    54121 aatgtattcc acttaactca agcatctctt tgtggatgtg attatgcagc tgtaacatat
    54181 gatttattta aaaaagcttc ttttaaccag cttacagtag atggtgccaa tacttttgaa
    54241 agagattggt ccaaggttaa taaatagtaa ctaagattta ggagggtttg cattgaacaa
    54301 aagattatta gatattgctg aattattact caacagctca gactatatta ctgtagatac
    54361 tattgccgaa gaattaaaag ttagtaataa aactattcgt aatgaccttg ttattttaga
    54421 tgaatggctt ttagagttta atttatccct agataaaaaa actggttcag gggttattat
    54481 tctaggtaat gaagatataa aacttaaagt tattagagat atcaatgata aatcaaattg
    54541 tatatatgca tactctcctg aagatagaaa aaggtatatt ctaagtaaat tatttattaa
    54601 aaattctaag tttaggattc gtgatatttg caatgaacta catgttagta gagctactgt
    54661 tcataaagac ttggtagtta ttcaaaattt tttgcaaaac tttaaagtga ctctagttag
    54721 aaaaaccaat aatggagtat atttagaggg aaaagagaaa gatattcgaa aagcagtttt
    54781 tgaacttgct actgttaata aaagttatac tgaacttaaa gaaatattat tctcgaactc
    54841 taatgaatgc aaagataccc catcaacaaa aatatttaag gagctattta actatgattt
    54901 tgagaaatta tctcaaatca ctctacacac cttacaagta gaaaaaagta accttagtga
    54961 tgagtattat ataaacttct taatacatat agctatatgt attaagagaa tttcaattaa
    55021 aagatatatt aacttatctg aaggtttttt tagagaatta ttagaacatg aatactttga
    55081 aaaatcaaaa attttatgtg ctaacctaag tgaagctttt gacgtagaat ttattgatga
    55141 agaggtctgt tatatacttc ttcatataga aggattactt aaatcaataa agaaaactac
    55201 tactcttgaa cctcttaaaa atgaagaaat tacagagtat aatctatctg aagcaatagc
    55261 tgttcattgg ggaagtatac taaatttaaa cttaaaggat gatttaatat taataaagtc
    55321 tcttgctaat caccttaaat cagttctaca cagaataaac tatggattta ctattacaaa
    55381 tcccatatta aatgatataa agagaacatt tccttatact tataaggctg ctaaagagtc
    55441 taatactgtt atcaaacagt tgatgaatta tgaagtcaat gatgacgaaa taggatatct
    55501 tgctttatat ttaatatcag caatagatag aagcaaatct cccctaaata ctattttaat
    55561 atgtcactgt agtgatggtg tatctaattt attagtccaa aagttaagtt ttgaatttaa
    55621 tcagattaat atagttaaat ctatacctct atcttctatt tcatttacga actttgatga
    55681 tgtggactta atactaacca ctgctcctgt agactttgaa cataatgctg aactgataaa
    55741 tataaatgct cttttaagta agaatgatat ttccagactt agtacaatta taaaaaaact
    55801 atattctgaa aaaaataaaa tattatctta taaataaaca aaaataggct tgtctcaaca
    55861 tagagataaa cctatttttc attgtccaaa tgataaaaat atatttgatt tcatagaaaa
    55921 agtgcctcat gaactaaaaa attcattttg agacaccttc attttttata tttaatcttt
    55981 ttatttaagc ttttatattt tttaaacttt tatatttttt aaacttttat atttaaagta
    56041 aattttatta tttttattga gcgcaagcat tatttatatt attaatttct tcagtattct
    56101 taccaaactt tgtagttaca aatcctattg tttttattat gagtaataag gatatcaatg
    56161 aatatataga tatcataaaa ttcttactta ttattccaga tgctactatt ataaatccat
    56221 ctagcataaa taatataact ggcaacttca gaaacttaat taccttatta ataagcattg
    56281 caactaaatc agttccacca gtactacagc cccttattat tgctatccct atagcagttc
    56341 ctaccaataa cccacctatc actcctgcta ctaatagatt gtcagttatg tgagtttgtg
    56401 gtattacttt taagaataat ggacccataa gagttatata taatgttttt attccaaaac
    56461 ctttccctaa aaataataat cccattacta aaagtggcat agatattcct aatgatatat
    56521 tctcaacagg cattgataag aatgtgctaa gtacgactga taatcctgtt atccctcctg
    56581 gagctaatgt atgttctgca aagaaaaaat taactgctaa tgccattaaa aatgctgata
    56641 ttgttatcag taaataatct tttatcattt cttttttcat ttaataacct cctatgatta
    56701 tattgtttca gtataaaaaa tcaattaaat tagtctagct tcctgtagtt tttcataaaa
    56761 ttaatgcttc cttaaaacat taagaaagca tctcttttat catagtttat tatcttattc
    56821 taaaccaaac tttgtagctg cttcatctat agcttttcta caattcttaa cacctgctgt
    56881 tataccatct ttatccttga atattgctga agataatatt acaacgtctg gattacattc
    56941 tattaatggc tccatattgt ctgctcttag tccaccatct attgctaatt cacacttagg
    57001 attcttttcg tctatcattt ttctagctct ttttactaaa tcaacagcac tctttctcca
    57061 tccccagtta tcttttccat cagtttcatc aacaccatga acgactattt gcaatctatc
    57121 tatatcatat atagcttcat caacaaaaca tagtggtgtg taacatccaa ttgttaatcc
    57181 tactttcata ccaaattctc tacagtaatt tattatgtat gcaagaggag cgcctaaaaa
    57241 atgttctgct ggtattatca acaagttaca tcctgctgct gctatttttt ctataaataa
    57301 tctatcacaa tctcttgtgt atatatggca ttctattggt ttatctgtca ctggtcttat
    57361 tgcttctatt atttgatgac cacccataag ttgcatattt tttaagtcat gcatatctgc
    57421 tgcatctgaa tgtacgtagt ctacacctgc atccattgct tctttaacta cggctgatat
    57481 atgaccgtaa tttacatgtg ctaatcctgc tgctattttt atgtgtttca tatttgtata
    57541 cctccatatt ttactttaat tagtattaaa tattagtttt tgatattttt taatccagta
    57601 ttacttttat ttagcattac ttttattgct gtatatattt cttttatgaa cttattctgc
    57661 ttttgttaac tgtgaactta ttttgctttg taaattaata ttacactgat atattttttt
    57721 tcacaacact ttgactactc cttaaaagta cctttatatt gtggtaaatt atagacatta
    57781 attgtatata aaaagttagt tttttaccac attcttatgg catttattgt ctatactaac
    57841 tcaattgata tagctagttc atttaatata taatcagact ataaatataa atttactcga
    57901 taaaaagtac cattcataac aatattaatg aaagggagat gtaaaatgag aaactgtatt
    57961 attgcccaat ctggaggccc tacagctgtt atcaatgctt ctgcagttgg tatttatgaa
    58021 aaaaatttag aaactaaata ctttgataaa gtatattttg gtatcaatgg gattgaaggt
    58081 atattaaaca aaaatataat agattcagac tccttaaaca aagataatgt attaaattta
    58141 aagcaaacac cttcgtctgg tcttggttct tgtagataca aactggctga ttataaaata
    58201 aattcttatg attatgatag agttataaat atactagatg aatatgaaat agatacatta
    58261 ttttatatag gcggaaatga ttctatggac acagtgatga aattttctca atactgcaaa
    58321 aacactactt gtaaaataaa atttataggg attcctaaaa caatagataa tgatttatta
    58381 ggaactgaca actgtcctgg atttggttct gctgcaaagc ttatttctac actaataatg
    58441 gaaacatact tagatttttc tatatacaaa aataatggta tatttatagt agaaaccatg
    58501 ggtagagata ctggatggtt agcagcttct tcttcactag ctagacttaa tgatactcca
    58561 cttgttgatt taatatacct tccagaaatt gatttctatg aagataagtt tatagaagat
    58621 gttagaaaaa tatttaaaga aaaaaataag gcttatatag ttgcatcaga aggtataagg
    58681 gataaatacg ggaactttat atgcgaacaa aaaacttctg ctcatgataa atttggtcat
    58741 tcccaattag gtggagtaag caactatcta aagaatctta tagtatcatc atcaattaca
    58801 acaagagtaa aaaccttaga acttggtgta atacaaagat gtgctatgca tttagcttca
    58861 gatactgatt taaaacaagc agctgaagtt ggtgcatatg ggatagaact atctaaaaaa
    58921 ggatatacag gagttatggc tatcattaat agaacatgca ataatcctta taagtatgaa
    58981 gttagtcata ctgatatttc taaaatagct aacaagacaa aatattttcc taaagagtgg
    59041 ataaatgaag aatgtaatta tataactgat gaagccctaa attatctata tccacttatt
    59101 caaggtaaac cagatatcaa atataaaaat ggactacctt atttttcaat tatttactag
    59161 gaaattatta taatttgata tacactcaat tctatcgctc ttaaatacct aagtggctgt
    59221 taaataaaat tataaattgt tatatgacag tcacttaatc gtttaattat ttaactgttt
    59281 agttttttaa ttttttaatc ttttttaata ctaatacgat ttgttgtaat aatatctcac
    59341 acccattttc attttggata gattataatt agttagacaa taaatatttt gtcatgatat
    59401 tattatatat agtgatacaa atagaaatac ttttatcatg actagaaaaa caaagattac
    59461 acaaaggatt taaaaatcaa acaacttcaa ttaaaataaa tggttaaaat aacttctaaa
    59521 tcaactacat aaacaataaa agaacataaa gtagttaaat ctacaattat aaaattggta
    59581 agactataat aacttaaata aaattaaagt taacaaagac tattaaagtt agcaaatatt
    59641 cctctaccta aataattgct ttttataaag agttatttat tttaatttat tttaattgaa
    59701 atacgatttt aactagaaca atagattaat gtaatattta atataaaata attataataa
    59761 ttgaaaagga agtaatctat gatgaaaata gcagtcataa caaccgaatt tttaaaagag
    59821 tttgtagata attctataaa aaagttaaat attaatgcag aaattgaaat atatatctat
    59881 agagattttt ctcatgttgg agatttatat ttagaaatag aagataggtt tgatggattt
    59941 gcagttagtg gccccatccc caaaaaagct attacaaaaa aggctggaac tattaaaaaa
    60001 cct
//
LOCUS       DAMJVX010000007        58584 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMJVX010000007 DAMJVX010000000
VERSION     DAMJVX010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 58584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.007"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            8..1204
                     /locus_tag="PC407_000362"
     CDS             8..1204
                     /locus_tag="PC407_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDF2495844.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1297..1821
                     /locus_tag="PC407_000363"
     CDS             1297..1821
                     /locus_tag="PC407_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDF2495845.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDTCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1846..2022
                     /gene="rpmF"
                     /locus_tag="PC407_000364"
     CDS             1846..2022
                     /gene="rpmF"
                     /locus_tag="PC407_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDF2495846.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2229..2786
                     /gene="fapR"
                     /locus_tag="PC407_000365"
     CDS             2229..2786
                     /gene="fapR"
                     /locus_tag="PC407_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDF2495847.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2797..3819
                     /gene="plsX"
                     /locus_tag="PC407_000366"
     CDS             2797..3819
                     /gene="plsX"
                     /locus_tag="PC407_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDF2495848.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3845..4831
                     /locus_tag="PC407_000367"
     CDS             3845..4831
                     /locus_tag="PC407_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDF2495849.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            4940..5869
                     /gene="fabK"
                     /locus_tag="PC407_000368"
     CDS             4940..5869
                     /gene="fabK"
                     /locus_tag="PC407_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HDF2495850.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            5885..6835
                     /gene="fabD"
                     /locus_tag="PC407_000369"
     CDS             5885..6835
                     /gene="fabD"
                     /locus_tag="PC407_000369"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDF2495851.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIEILLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6835..7584
                     /gene="fabG"
                     /locus_tag="PC407_000370"
     CDS             6835..7584
                     /gene="fabG"
                     /locus_tag="PC407_000370"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDF2495852.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTRVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7638..7862
                     /gene="acpP"
                     /locus_tag="PC407_000371"
     CDS             7638..7862
                     /gene="acpP"
                     /locus_tag="PC407_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2495853.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            7891..9129
                     /gene="fabF"
                     /locus_tag="PC407_000372"
     CDS             7891..9129
                     /gene="fabF"
                     /locus_tag="PC407_000372"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDF2495854.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9355..11073
                     /locus_tag="PC407_000373"
     CDS             9355..11073
                     /locus_tag="PC407_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2495855.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLNIGSYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMKSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDNIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11104..13104
                     /locus_tag="PC407_000374"
     CDS             11104..13104
                     /locus_tag="PC407_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2495856.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKEKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNVRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTKKEFEEILRILYERRESSSGVGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13452..13697
                     /locus_tag="PC407_000375"
     CDS             13452..13697
                     /locus_tag="PC407_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2495857.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13732..14457
                     /locus_tag="PC407_000376"
     CDS             13732..14457
                     /locus_tag="PC407_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2495858.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEEENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14479..15792
                     /locus_tag="PC407_000377"
     CDS             14479..15792
                     /locus_tag="PC407_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2495859.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVCGENLKKIV"
     gene            16091..16537
                     /locus_tag="PC407_000378"
     CDS             16091..16537
                     /locus_tag="PC407_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2495860.1"
                     /translation="MGGETHFLSFGKDEYKISLEEQENAIESAKASDNSVKLIAFIDD
                     EIVGIATIDSNQKAKGKHVGVLGIVVKEKYWGIGLGKRLMLDLIEWCKSNGITKKITL
                     VTNEENYNAIGLYKKVGFEVESILKKECYYNGVYTDLIGMSLLLGI"
     gene            16626..18611
                     /locus_tag="PC407_000379"
     CDS             16626..18611
                     /locus_tag="PC407_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDF2495861.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTITLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIEEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18835..19803
                     /gene="spoIIIAA"
                     /locus_tag="PC407_000380"
     CDS             18835..19803
                     /gene="spoIIIAA"
                     /locus_tag="PC407_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HDF2495862.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIDD
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19793..20314
                     /locus_tag="PC407_000381"
     CDS             19793..20314
                     /locus_tag="PC407_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HDF2495863.1"
                     /translation="MQIKIIIIALLVGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20331..20528
                     /gene="spoIIIAC"
                     /locus_tag="PC407_000382"
     CDS             20331..20528
                     /gene="spoIIIAC"
                     /locus_tag="PC407_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HDF2495864.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20544..20930
                     /gene="spoIIIAD"
                     /locus_tag="PC407_000383"
     CDS             20544..20930
                     /gene="spoIIIAD"
                     /locus_tag="PC407_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HDF2495865.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            21015..22220
                     /gene="spoIIIAE"
                     /locus_tag="PC407_000384"
     CDS             21015..22220
                     /gene="spoIIIAE"
                     /locus_tag="PC407_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HDF2495866.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22280..22897
                     /locus_tag="PC407_000385"
     CDS             22280..22897
                     /locus_tag="PC407_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HDF2495867.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            22909..23463
                     /locus_tag="PC407_000386"
     CDS             22909..23463
                     /locus_tag="PC407_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HDF2495868.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKKQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23490..24179
                     /locus_tag="PC407_000387"
     CDS             23490..24179
                     /locus_tag="PC407_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HDF2495869.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSDSSKTEDSSNVDKQGGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24289..24651
                     /locus_tag="PC407_000388"
     CDS             24289..24651
                     /locus_tag="PC407_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2495870.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24736..25242
                     /gene="nusB"
                     /locus_tag="PC407_000389"
     CDS             24736..25242
                     /gene="nusB"
                     /locus_tag="PC407_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDF2495871.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25226..26296
                     /locus_tag="PC407_000390"
     CDS             25226..26296
                     /locus_tag="PC407_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HDF2495872.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26293..27498
                     /locus_tag="PC407_000391"
     CDS             26293..27498
                     /locus_tag="PC407_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDF2495873.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVMVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27500..27715
                     /gene="xseB"
                     /locus_tag="PC407_000392"
     CDS             27500..27715
                     /gene="xseB"
                     /locus_tag="PC407_000392"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDF2495874.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27718..28605
                     /locus_tag="PC407_000393"
     CDS             27718..28605
                     /locus_tag="PC407_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDF2495875.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28635..29072
                     /locus_tag="PC407_000394"
     CDS             28635..29072
                     /locus_tag="PC407_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HDF2495876.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29106..30971
                     /locus_tag="PC407_000395"
     CDS             29106..30971
                     /locus_tag="PC407_000395"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDF2495877.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANNNED
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31104..31916
                     /locus_tag="PC407_000396"
     CDS             31104..31916
                     /locus_tag="PC407_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDF2495878.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEYTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            31936..33615
                     /gene="recN"
                     /locus_tag="PC407_000397"
     CDS             31936..33615
                     /gene="recN"
                     /locus_tag="PC407_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDF2495879.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLKSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGSFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33830..34696
                     /locus_tag="PC407_000398"
     CDS             33830..34696
                     /locus_tag="PC407_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495880.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECTIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGDISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKAYDNIRLEKVDIIVNEGKKD
                     TIYKAQLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34787..35278)
                     /locus_tag="PC407_000399"
     CDS             complement(34787..35278)
                     /locus_tag="PC407_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2495881.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIVEAKKIGYTHMRLDTLPSMKSAQGLYEKFGFYDIELYTYNPIEGARYME
                     LKL"
     gene            complement(35339..35779)
                     /locus_tag="PC407_000400"
     CDS             complement(35339..35779)
                     /locus_tag="PC407_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2495882.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGCGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFKFNGELDYGGEKVMVLKSN"
     gene            35940..36998
                     /locus_tag="PC407_000401"
     CDS             35940..36998
                     /locus_tag="PC407_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDF2495883.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVGYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37140..37964
                     /gene="spo0A"
                     /locus_tag="PC407_000402"
     CDS             37140..37964
                     /gene="spo0A"
                     /locus_tag="PC407_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HDF2495884.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38377..39498
                     /locus_tag="PC407_000403"
     CDS             38377..39498
                     /locus_tag="PC407_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495885.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39516..40391
                     /locus_tag="PC407_000404"
     CDS             39516..40391
                     /locus_tag="PC407_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HDF2495886.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIVLKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNSLGNTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40415..41107
                     /locus_tag="PC407_000405"
     CDS             40415..41107
                     /locus_tag="PC407_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2495887.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41115..41939
                     /locus_tag="PC407_000406"
     CDS             41115..41939
                     /locus_tag="PC407_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2495888.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            41944..43011
                     /locus_tag="PC407_000407"
     CDS             41944..43011
                     /locus_tag="PC407_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HDF2495889.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGKIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43013..43549
                     /locus_tag="PC407_000408"
     CDS             43013..43549
                     /locus_tag="PC407_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2495890.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43617..44222
                     /locus_tag="PC407_000409"
     CDS             43617..44222
                     /locus_tag="PC407_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HDF2495891.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTVSIGIIKFLAK"
     gene            44234..45148
                     /locus_tag="PC407_000410"
     CDS             44234..45148
                     /locus_tag="PC407_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2495892.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45190..46362
                     /locus_tag="PC407_000411"
     CDS             45190..46362
                     /locus_tag="PC407_000411"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDF2495893.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46409..47221
                     /locus_tag="PC407_000412"
     CDS             46409..47221
                     /locus_tag="PC407_000412"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDF2495894.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47425..48750
                     /locus_tag="PC407_000413"
     CDS             47425..48750
                     /locus_tag="PC407_000413"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDF2495895.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEVDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSIKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            48963..50042
                     /gene="mltG"
                     /locus_tag="PC407_000414"
     CDS             48963..50042
                     /gene="mltG"
                     /locus_tag="PC407_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDF2495896.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            50214..50888
                     /locus_tag="PC407_000415"
     CDS             50214..50888
                     /locus_tag="PC407_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDF2495897.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFASIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            50875..52122
                     /locus_tag="PC407_000416"
     CDS             50875..52122
                     /locus_tag="PC407_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2495898.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRAPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            52194..53858
                     /locus_tag="PC407_000417"
     CDS             52194..53858
                     /locus_tag="PC407_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDF2495899.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(53955..54239)
                     /locus_tag="PC407_000418"
     CDS             complement(53955..54239)
                     /locus_tag="PC407_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2495900.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(54262..55779)
                     /locus_tag="PC407_000419"
     CDS             complement(54262..55779)
                     /locus_tag="PC407_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2495901.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKACDLDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAIYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            complement(55904..56725)
                     /locus_tag="PC407_000420"
     CDS             complement(55904..56725)
                     /locus_tag="PC407_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2495902.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETK
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKSLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            56916..58343
                     /locus_tag="PC407_000421"
     CDS             56916..58343
                     /locus_tag="PC407_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HDF2495903.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISANALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSINKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKLVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTIAKNNAIVLVGNDSNKSV
                     LKSTIKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYE
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKIYN"
BASE COUNT        23452 a         6090 c        10720 g        18322 t
ORIGIN      
        1 aataataatg aaaatattag tattaaactg tgggagttct tcattaaaat atcaattaat
       61 agatatgaat aatgaagaag ttttatgtat aggattggtt gaaagaatag gaatagaagg
      121 ttctatatta aaacatgaaa aagcaggtag agatgataaa tacgttgttg aacaaccaat
      181 gaaggaccac aaagatgcaa tagcattagt tttagaagct gttgctcatc cagaatttgg
      241 tgcagttaaa gaaatgaaag aaatagatgc agtaggacat agagttgtac atgctggaga
      301 aaaatttgca acttcagtag taataactcc agaagtagaa gaagctttaa aagaatgtat
      361 agatttagct ccacttcata atccagcaaa cataatggga atagatgctt gtaaagctat
      421 acttccagat gtaccaatgg taggtgtatt tgatactgct ttccatcaaa caatgcctaa
      481 atcttcatac ttatatggtt tacctcatga gctatatact aaatatgggg taagaagata
      541 tggattccac ggaacttctc ataattatgt atctcagaga gcagcagaga tattaggaaa
      601 agatataaaa gatttaaaaa tagtaacttg tcacttagga aatggtgctt ctatagctgc
      661 tgttgatggt ggaaaatgtg tagatacatc tatgggattc actccattag aaggattaat
      721 aatgggaact agatgtggag atatagaccc agctatatta ccattcttaa tgagaaaaga
      781 aggtttagat gctgatggat tggataaatt aatgaataaa gaatctggag tatatggaat
      841 gactggaatt tctagtgact tcagagatat agaagatgca gctaaaaatg gagacgaaag
      901 agctcaggct acattagaag cgtatgttaa aaaagtacaa aaatatatag gcgcttatgc
      961 tgctgaaatg aatggattgg atgttgtagt atttactgca ggcgttggtg aaaatggaaa
     1021 ggctataaga gcagatattg cttctaacat ggagttttta ggaatgaaat tagataaaga
     1081 agctaatgat gttagaggaa aagaaacagt aatatctact gctgattcta aagtaaaaat
     1141 gcttttaata ccaactaatg aagaattaat gatagctaga gatacattaa gattggtaaa
     1201 ataattaaat taaacctatg cctatcaagt aaaaatactt gacaaacaaa tgctagattt
     1261 gtataataaa tttggtgata tgttgaggtg aacttaatga aagttagcat agaaaaaata
     1321 aatagaaaag aaactgacaa aatagacttg aatttttgtg aaaaaattga tactattagt
     1381 tattgtgatg agatttacaa attagtatca cctgtaaatt taaaagggaa agtgtcaaag
     1441 actaataaag gtttgtatct agatatagat gtcaatttta caattgttga cacttgttca
     1501 agatgtctta aagaagtaga aataccacta gagtattcta tacaaggttt tttagtaaaa
     1561 gaagaagatt atgatgaaga tgagtttgag gaatttgatc cttttatatt cgatggtgaa
     1621 gaaatcgacc ttattgatat aatagaacag acattagatt ttaatgtacc tcataaagtt
     1681 ctttgtagtg aaaactgtaa aggtctttgt caagtatgcg gggcaaactt aaatgaagaa
     1741 gagtgttctt gcagtgaaat tacaaacgat gaggaataca tagatcctcg ttttgctaaa
     1801 ttaaaagatt tatttaatta aattttgact aaggaggtgg cgtaaatggc agtaccaaag
     1861 cgtaaaacgt ctaaatcaaa cacaaaaatg agaagagcag ctaactcaaa gatggaagca
     1921 acaggatttg taagttgccc acaatgtcat gagccaaaat taccacatag agtgtgtcca
     1981 gactgtggat attataaagg taaagaagtt gtgtctaagt aatagcttct aaaaagcatg
     2041 tagagtatac tctacatgtt ttttttgtac atataaaaaa ataagatgat ttactagaga
     2101 aaataagtgg attttatgca tattaaaaaa tagaaaaata ataatataag tattgaaaat
     2161 ttttgtgtgt tactatatac tactattagt agctggtact aattacagct ataaaatttg
     2221 gagggaacat gaaaaagaaa agtaaagccc aaagacaaaa agagcttata gatatgctaa
     2281 agacagatcc tttttatact gatgaagaat tatcaagtct gtttgatgta agtatacaga
     2341 ccataagatt agatagaatg agtctaaaca taccagagct aagagaaaga gtcaaatcaa
     2401 tagcagaaac tcagagttca aaagtaaaga ctttaggagt aaaggaaatt acaggagaaa
     2461 tcattgattt atctgtaggg cgattaggta tatctatgtt agaagttaca caggacatga
     2521 tttactctaa gactaatact ttaaaagata cgtatatttt ttctctagca gattcattgg
     2581 caatggctat aatagatgca cctaaggtta ttatgagagt tgcaaatgta aaaagtttta
     2641 agttgataga acaacaagat agacttatag caaaagcaga agtgtataga aatatagata
     2701 aaaagcatta tgtaaaagtt gttatcaaca ataaagccca agagcaaata tttagaggaa
     2761 agtttatctt tgaagaatta gattaggagg gaaattatga aaatagtaat tgatggaatg
     2821 ggtggagata atgcacctaa atctaatgta gaaggcgcag taaatgctat aaaagaatat
     2881 caggttgatt taataatcac aggagataaa gatttacttg aaaaggaatt ttcaaattat
     2941 gaatttgata gaaataaatt agagatagtt catactactg agattataga aaacgaagat
     3001 aaaccagtaa aggctataag aagtaaaaaa gattcatcta tggtagtagc attaaatctt
     3061 gtaaaagaag gaaaggcaga tgctataata tcagctggaa atacaggagc cttacttgct
     3121 ggtggattat ttgttgttgg tagaataaaa ggaatcgata gaccttgctt atgttcagca
     3181 ataccaaatg taaagcgagg aatgacactt atagcagact gtggcgcaaa tgcagattgt
     3241 aaacctaaga atctagttga atttgcagct atgagtaata tttattcaag aaaagtttta
     3301 ggtcttgaaa atcctaaggt agcattagca aatgttggac tagaagaagg caaaggcaat
     3361 gatttagtca aaaggtcata tgaagaaatt aaaaaattag atttaaattt tatagggaat
     3421 gtagaagcta gagaagttat aaatgcatac acagatataa tcatatgtga tggatttaca
     3481 ggtaatatat tacttaaatc tgctgaaggt gtagcacttt cagttatgag tcttataaag
     3541 gaaactttta tggccagtac aaaaagtaaa ataggagcat tacttataaa ggatgattta
     3601 agaaaattaa aaagttttat agattattct gagtatgggg gagctccact tttaggtcta
     3661 aatggaggag ttataaaagc tcatggtagt tctgatgcta aagctataaa aaatgctata
     3721 aatcaaggaa taaaattttc aaagggaaaa gtcgttgaag atattaacca atttataagc
     3781 aaatacaatg aagaaaataa aaataacgaa gatgaataaa ctcgttttgg aggtttaata
     3841 tataatgaat acaaaggctg gaatattagg agtaggaagt tatctacctg agcaatcata
     3901 tgataatttt cacttcgaaa aaataatgga tacatcagat gagtggataa gtactagaac
     3961 tggtattaag gaaagaagat ttgccaaaga gtctgaagca acttctgact tagcatctaa
     4021 agctgcctta aaagctatag aatgtgcaaa gttaaacgta gaggatatag agctgattat
     4081 attagctaca attactcctg atatgtcttt gccttcaact gcatgtatag tacaagatgc
     4141 aataggagca gtgaatgcta ctgcatttga tatatcagca gcttgttctg gatttgtata
     4201 tggagtaact attgcaaagc aatttgtgga aacaggttgc tataaaaatg tgcttgtaat
     4261 aggagcagaa acttgttcta agtttttaaa ttatgatgat agaacaacag cagtattgtt
     4321 tggagatgga gctggagcag ctgttattgg tcctgtaaat gagggtggaa tactgtctac
     4381 acatatgggc tctgatggta aaggaaaaga ttgtttaaaa gtacctgctg gaggttcaag
     4441 acttaaggca agtaaagaga ctgtagaagc taatctacat acaatagaaa tggcaggtag
     4501 tgatgtgttt aaatttgcag ttagaaagat ggcagaaact tctttaagag ctcttgaaaa
     4561 agcaaaccta aatacaactg atatagacta cttagtgcca catcaagcta atataagaat
     4621 catacaagct tcttctaaga gattagagct tgatatgaaa aaagtttatg taaatataga
     4681 caaatatgga aatatgtctg ctgcatctat accagtagca ttagatgaag cttatagaga
     4741 aggtaaaatt aaaaagggtg ataatgtagt tttagttggc tttggtggag gtctaacttg
     4801 gggggcttca gttgtcaagt ggactttata gtataaaata tctaaaatga tttaattaac
     4861 aaaattaaac ttatacacaa aataaagttg attaataata aaaagtttac ttttatgatt
     4921 tcaagtgaag gagaattgta tgaataaaat ttgcaaaata ttaaatataa aatacccagt
     4981 tatccaaggg ggaatggcat gggtagctac tgcatcatta gcaagtgctg tatctaatgc
     5041 aggaggactt ggcataatag cagcaggaaa cgcaccaaaa gaagctataa agaaagaaat
     5101 tgttgagtgt aaaaaattaa cagataaacc ttttggagta aatgtaatgc ttatgtcgcc
     5161 atttgttgat gatataattg atttgattat agaagaaaaa gttcaagtta ttactactgg
     5221 tgctggaaat cctgcaaaat atatggatag attaaaggaa gctggaacaa aggttattcc
     5281 tgtagtacct acaatagctt tggcacaaag aatggaaaag ttaggagcta cagcagtaat
     5341 agcagaaggt actgaaggtg gaggacatat aggagaactt actactatgg tcttagttcc
     5401 acaagttgct gatgctgtaa acatacctgt aatagctgct ggaggaattg tagatggtag
     5461 aggaattgca gcatcatttg cattaggtgc cagtgcagtt caagtaggaa ctagatttat
     5521 ttgcagtgaa gagtgttctg tgcattcaaa ctataaaaac ttagtactaa aagcaaaaga
     5581 tagagatgca attgtaacag gaagaagtac tggtcatcca gtaagaacat taaaaaataa
     5641 actatcaaaa gaatttttaa agatggaaca aaatggagct actcctgaag aattggataa
     5701 aaaaggtaca ggagctttaa gatttgcaac agtagatgga gacatagaaa aaggttcatt
     5761 tatggcaggt caaagtgctg ctatggtaaa agaaataaca ccttgtaagg aaattataga
     5821 ggctatggta aatcaagcaa gagagattat gccagcaata gaactgtaaa tcaggaggag
     5881 caagatggga aaagttgcac tagtattccc tggtcaagga gctcaatatg taggtatggc
     5941 caaggattta tatgaaaata atgctgttgc taaaagtgta atagatgagg ctagtgatgc
     6001 cttaaatatg gatttaaaaa accttatgtt taatggaaat gaagaagaac tttctaaaac
     6061 tgaaaacact caacctgcta tggttacaca tagtgtagca gtattaaaag cggtacaagc
     6121 tcaaatagat ttaaaatata atgcatgctt aggattatcc ttaggagagt atagtgcgtt
     6181 ggttgcagca gatgcaattg actttaaaga tgctgtatgt ttagttaaaa aaagaggtaa
     6241 gtttatgcag gaaacagttc cagcaggtgt tggagctatg gcagcaattt taggtttaga
     6301 tagaaatgta cttgaaaatg tggttaaaga tatacaagga ggtattgtag aagtagcaaa
     6361 ttacaattct cctggacaaa tagttatatc tggtgaaaat gaaaagatag aagaagcaat
     6421 ggtaaaatgc aaagaagcag gagcaaaaag ggcagttaaa ttaaatgtaa gtggaccttt
     6481 tcactcatca atgttaaaag aagctggtgt aaaattggcc agtgaattag aaaaagttaa
     6541 cataactaaa cctaaagttg atgtggtggc aaatgtaaat gcagattact ataaaaacga
     6601 aggtaaagac ctgcttataa aacaagtaag ttcatctgtt ctttgggaag attctataga
     6661 aatactttta aatgatggat atgatacttt tatagaaatg ggacctggaa aaactttaaa
     6721 agcatttatt aagaagatag cttccaataa aaaagcaaat gtcaatattt ataatataga
     6781 tggtatagat tctcttaatg aatttgtaca aaattataga aatggagaga tataatgata
     6841 aatcttacag gacaagtagc agttgttact ggtggctcta ggggtatagg aaaagaaata
     6901 gcaaaaaaac tagcatcttt tggagctgat gtagtaatca attatacttc taaagaagat
     6961 gaagcactaa aaactaaaaa tgaaatagaa agtatggggg taaagtgtac ctctataaaa
     7021 tgtgatgtgt ctaaatttga tgaagtgaat caaatgatag attctgttgt aagcgaattt
     7081 ggaaaaattg atatattggt taataatgca ggcataacta aagatggtct gcttatgaga
     7141 atgaaagaag aagattttga tagagttata gatataaact taaaaggtgt ctttaattgt
     7201 acaaaagcag ttactaaacc tatgatgaaa aagaagtatg gaagaataat aaatatgact
     7261 tcagtagttg gaattatggg taatgcaggg caaactaatt attgtgcatc aaaagcaggt
     7321 gtaattggat ttacaaaagc ttctgcaaga gagttagcat caagaaacat aaatataaat
     7381 gcagtagcac ctggatttat agaaacagat atgactagag tactaagtga tgatgtaaaa
     7441 gaatcaacac tagcaaacat accaaagaaa tcttatggta aaccagaaga tgtagccaat
     7501 gccgtagcat ttttagttag tgacatgtca agttatataa caggacaagt aataaatgta
     7561 gatggtggaa tggtaatgca ataataattt aaaaagtaga gaaatctatt aaaataaaaa
     7621 attttaggag gtacacaatg tttaataaag ttgtagaaat aataatagag caattaggag
     7681 tagaagataa agaaataact atggagacat cattaatgaa ggatttagaa gcagattcat
     7741 tagatgcagt agaaataata atggcacttg aagatgaatt tggtatagaa ataccagata
     7801 cagaagcaga aaacttcaaa tctataggag atatagttaa gtatatagaa gctaataaat
     7861 agtcgagctt taaaaaagtg gaggtatccg atgaataaaa gagtagtaat aacaggactt
     7921 ggatgtgtta cgccgttggg aacgggaaaa gaagagttct ggagtaatat aaaatctgga
     7981 gtatctggta tagataaaat aactaatttt gatgctagca catatcaaac tcagatagca
     8041 ggagaagtta agaattttca tccagaagaa tatataagta aaaaagaatt gaagagatta
     8101 gataaatttg ctcaatttgc aatagtatct gctaaattag cagttgaaga tgcaaatctt
     8161 gatttagata aagttgatag agaacgtttt ggtgttataa taggttctgg aataggtgga
     8221 gttgaagcta tagaaactca acataaaatt cttttggaaa aaggaaataa aagagtaagt
     8281 tcactttttg tacctatgat gataggaaat atggcagctg gtcaagtttc aatattttta
     8341 ggagcaaaag ggcctaacac taatgtatgt acagcttgtg catcaggaac tcattcaata
     8401 ggtgatgctt ttaaggtaat tcaaagaggt gatgcagaca tcatggtagc agggggctct
     8461 gaagctgctg taacaggtct tgcttttgct ggattctgta acatgaaggc tatgtcaact
     8521 agaaatgatg accctaaaac tgcatcaaga ccgtttgata aagacagaga tggttttgta
     8581 atgggagaag gagcaggtat tgttatctta gaagacttag aacatgcttt agcaagaggg
     8641 gctaaaatat atgcagaagt agttggatat ggtttaactg ctgatgcata ccatatgaca
     8701 actccagctg aaaatggaga gggagcagct aggtctatga atatggcttt gaaagatgga
     8761 aatgttccat tagaagaagt agattatata aatgctcatg gaacttcaac ttattacaat
     8821 gatttatatg aaactatggc tataaaaact gtatttggag aaaaggcata tgacttatgt
     8881 gtgtcatcaa caaaatctat gactggtcac ttattaggag catcaggtgc tatagaggct
     8941 gttgtttgtg ctatgagtat agaagatagt tttgtgcctc caactataaa catacaagaa
     9001 gttggtgaag atttggattt agattatgtt ccaaatcaag gtaaagaaaa aaatattaga
     9061 tatgcactat caaattcttt aggatttggt gggcataatg ctacaatagt actaaaaaaa
     9121 tatgtataaa taaatattaa atataaaact aaaaaacaag cacttgtttt aactgtataa
     9181 atttatatta gcttaaattt aaagtctcct aaattatttt aggagacttt tttattaaga
     9241 tttaagtcta gcatatgttg taaatcattt tgatatattg tattattaat tattaagagg
     9301 ttatttatat atgcaagttt tcattctaca atatacaaaa ggaggactaa aattttgaat
     9361 tttagactac caaagaaaag gttacccaaa aagataattt ttatatgtat aatacttatt
     9421 agtattattt tgtttataaa gtatgaccaa aatggattga aagaaagaga aattataaat
     9481 aagggaaaat atatgtctga aagccttgtg gcagataagg agtatgatga agcaaaaaaa
     9541 gtggttaaga cttctttcga atctattcca gaaaaaaaat acaataaagt atataatgaa
     9601 gtttgggata tgttaaagac tatttcatat gttccagatg gaacgaagat agtcattgat
     9661 tctttagaaa agatgagaga atcagatgtg aaattatctg atgaatgtag atttaatata
     9721 gaaaaaagat tagcatcagt gtatataatg gataataatt attctaaggc agtagattta
     9781 actgtaaagt ctataaaatt ggctgagaaa ttggggaata attatgaaaa agcaagacta
     9841 gatgttgatt tggcgagcat atttttaaat ataggtagct atgaaacagg agagaaatta
     9901 ataaaggatt cttttaaaat agatattgat gaaggtgaaa aaaatggaat ggttagatta
     9961 tatgctatta taaatctagc agaaatatat gtagaagagg gagaatatga taaagctatt
    10021 gaaataagta gcaggataaa agattataaa aagtttgtaa atacggataa ttacaatgat
    10081 tttgatataa tggcctctat aatgaagtca aatgcgtatg tgggcaaaaa taatatacaa
    10141 aaggcaaaga cttttttaga aaaagctgat tatcttataa aatcagatag aacagtttat
    10201 atattagata aagatatgta ctattatatg gcaatgggaa acttagaata taaaagcgaa
    10261 aattataatt ctgcaataga taattataaa aaaagtttag agatatctac caaaagaaag
    10321 ttaactcagg agaaaataaa aaatttaaat aagatattaa atatatatga aaaacaagga
    10381 aatatagaat ctttaaatga gtatcaaaag atgttaataa atgaatataa agagaacaaa
    10441 agtataagag attcagagac tagtttctat ataattgata aagtatcaaa cgaaagtaag
    10501 ctatttgaaa aaactaaaaa agaaataaaa tattataaaa tattatttac agtaatttta
    10561 attgctatat tagggtctac ttttttatac aaccgattga agtattttaa agcacaaaat
    10621 ttacatgatg gtcttacaaa tgtttataat agaaaaagtt ttgatattat gtatgaaaag
    10681 tacagagaga aagatgataa ttttgcactt gtgatgatag atattgataa tttcaagctc
    10741 ataaatgata attatggaca taaatttggt gacactgtta taaagggaat aacaaatacc
    10801 atttgtgtta tgttagaaca agatgataaa gtctttaggt acggaggaga agaattttct
    10861 gtattaataa gaaataaatc tggtgaggaa gtagcagata taattgacaa tataagggtt
    10921 gcaattgcaa ataaaaagtg gaatgatgat gtgactgtga ctataagtgc aggtgtagct
    10981 catatttcag atagcaaaga tgtactagaa gaagctgata aaaatttata taaagcaaaa
    11041 caacttggta gaaataaagt agtatataag taattatata ctatataaat aagaggtata
    11101 aatatgaaac aatcaatagc tatagtaact gataaaataa ctaaactatc atctttctta
    11161 gaagaaaata tacgtttggt tttaggtaat tatattgata taaaccacta ttatctggaa
    11221 gttttaaaag agaatgataa aatacaaggt gattttgtac tagtaatgaa tgatgataga
    11281 ttaaacagta tgagaaagca tttagtacaa gatagtaaga ttatagtgat aaggagaaca
    11341 cttaaagaaa atgaaatata tggattattt tcaataccaa atggaactga agtattggtt
    11401 gtaaatgata caaaagaaac taccttagaa acgataagct tattttacaa aataggagta
    11461 aaaaatttaa agtttactcc atatattgaa ggaaaaattt ataaaaatat agacatagct
    11521 ataacgcctg gagtaaagga aaaagtacca agttatataa ataaagtaat cgaccttggt
    11581 aatagatata ttgatatatc tacttttata gaaataataa atggtcttag aattgatgtt
    11641 aaagaaatac gtaaaaattt aatggcatat tctgagagtt taataagttt agataatgga
    11701 gttaaagata attataggca actgttttta aaaatagaag aacaagatgt tatattgaat
    11761 ctatcaaaag atggaataat atttacttca gcagatggta taataaacac atttaataaa
    11821 gaagctttaa aaattttagg tataaatgaa aatccacaag gcaaaaacat agaagatatc
    11881 atttctgaag atttaaatat tttacttgaa gacaaagaaa taattgatga agtggtattt
    11941 acaaataaaa aatatataaa tgtaaataaa aaaagtattt tttctatggg gaataaagct
    12001 gggatatatt ataacttaca agaaataacg tatataaaaa aattagaaca aaatcttact
    12061 aataaattaa gagaacaagg tcagatagca agatatacat ttaaagatat aaaaactaaa
    12121 aataaaaata tggtaaagtg tgtagaattg gctaaaaaaa tatctaaatc agacctttct
    12181 gtattaataa taggagaaag tggtactggt aaagaattat tatcacaatc aattcataat
    12241 gcttccaata gaagtaatca accatttata gcggttaatt gtgcagcagt tccagatagt
    12301 ctattagaaa gtcaactgtt tggatatgaa aaaggctctt ttacaggagc tttaagagaa
    12361 ggaaaaaagg gtctatttga acttgctaat aatggaacta tatttctgga tgaaattgga
    12421 gatatgcctc cattgctaca gacaaagtta ctaagggtat tacaagaaaa gcaggttatg
    12481 cctataggtt cccataatgt tataaacata gatgttaggg tcatagcagc aacaaacaaa
    12541 aacttactaa agatgattag agaaggaaaa tttagagagg acctgtatta tagactaaat
    12601 gtattgccaa tagatgtacc atcattgagg aatagaaaag aagatataat aacacttatg
    12661 aatttctttt tgaaaaacaa tgtaagactt tcgccagaag ttataactat tttagaaaaa
    12721 tataattggc caggaaatat aagagagtta caaaatgttg cttcttatgt aagtctaatg
    12781 tgtgatagta tggtttttag agatgactta ccaccatata taagggtaga atgtgctaac
    12841 aattatgaag atggatttgg ttatacaaaa aaagaatttg aggaaatact gaggattcta
    12901 tatgaaagaa gagagtcatc ctctggagtt ggaagagggt ttataatcga aaagcttata
    12961 caggaaggga ttgagatttc tgaaagcaaa gttagaaagg tgctatctta tttaagtgaa
    13021 tgtggatata tatcatctaa atctggtaga ggaggaagtc aaataacctc agaggggata
    13081 aaactttata ataatataaa ttaagctaag tatttgatat tgaaatcaca tattttgaat
    13141 ttagatattt aaataataaa cttttatatt tatagagatt aatttatact atgtcagaaa
    13201 gaatattgta atttataatt aaatattaat aggtttttaa aataggatta gataggtaat
    13261 aggttaagtt agaataaccc attacctatt tttatttgta agaaaagtgt acatagagtt
    13321 aaaagtaaaa aatacaacta ataaaactat tgaaaacact agataaataa taaataaaaa
    13381 taattataaa ttttggcata tatattgcta atcatagaga taagacgaca aaataattgg
    13441 gaggtttaat gatgaaatac aaaacaacag ggacttgtgc tacagaaatt gaatttgaag
    13501 taaaagaaaa taaagttaca aatgtaaact ttataggtgg atgtgatgga aatttaaaag
    13561 ggctaaaagt attagttgaa ggcatgaata tagaagatgt aatacaaaag ttaaaaggaa
    13621 tagagtgtaa aacaaagcca acatcttgcc cagaccaact gtctctagca ttggaaaatt
    13681 atatgaatat aaagtaatac ataaaacaat cgattagtta ggagtttaat tatgaaacca
    13741 ataataggga ttttaggaaa tttaataata atggaaaatg gtatgtttcc aggtcttgaa
    13801 aggtcatatg taaataatga ttatataaat gcagtgctaa aaggtggcgg tagtccagta
    13861 ataattcctg taaatacaga taaagaagtt ataaaaaaac agattgaaat ggttgatgga
    13921 gtcttaatat ctgggggatg ggatatcaat cctcaactgt atggagaaga aacaagagaa
    13981 gaaacgacat ttatatatcc agaagtggat gaatttgatt taattgcaat aagtatagca
    14041 ttagaactta aaaagcctat attaggtgta tgtaggggac tacaaatttt aaatgtaagt
    14101 ttaggaggaa ctttatatca agataataat cttatagaag gaagttatat aaaacataca
    14161 caatcttcta aaagacatgt agctactcat aaagtcgatg ttaaagaagg aagtatttta
    14221 gagggaattt taggaaaaca attacttaca aatagttatc atcatcaaag tgtcaatcag
    14281 cttggaaaag gattaaaagc catagcatac tcaaaagatg gtataataga agctattgag
    14341 aaagaggagg aaaattttgt agtaggagta cagtggcatc cagaaatgat ggtagattac
    14401 tgtgataaaa tggaaaaact atttaaatat tttataaata tttgttcaaa tttataatat
    14461 accttggggg gattgaacat gaacgaaaag aacaaaatgg gattaataag tattatactt
    14521 ttaggtatta atgcagtagt aggagctgga gtttttctat taccaggaga tgcaatgaaa
    14581 tcatttggtg tagcaagtat atttgtatat atatttgata tgttgttagt tttatctatg
    14641 gcattctgtt ttgctgaggt tgcaggtaaa ttcaataaaa atggcgctgc ctatgtatat
    14701 accaaagaag cttttggaga tttttgtggt tttgaagtag gtcttatgaa atgggtaata
    14761 ggatgtattt cttggggagc tttaatagta ggttttccta catcattatc agcagtatgg
    14821 gctccagcag gtgaaccaca tatacaaaaa attataattg tagccatgat agttgggctt
    14881 acaattataa acttattagg tgtatctcta tcaaaaattg tacaaaatgt tataactgta
    14941 ggtaaactaa ttccattaat actatttata ggaataggta tattttttat aaaaggagtt
    15001 aattttactt cttcaacaat ggtacctcca ggagctggag caacagagtt tggagcagca
    15061 gcacttttaa tgttttattc atttacagga tttgaatcta tagcagttgc agcagaagat
    15121 atggaaaatc cacaaaaaaa tataccaata gcaattataa gtgtaatagt aattgcatca
    15181 ataatatata tattaaatca agttgtatgt gttggtatac taggagattc gttatctagt
    15241 acatctacac cagtggcaga tgctgcaaga atatgctttg gaaatatggg agctggattg
    15301 gttacctttg gaacattggt ttctgttggt ggaatttgta tgtgtggtgc ttttgtaaat
    15361 ccaagaagtt gtgtagcttt agctgatgat aaaatgttac caagaatatt tgcaagaaaa
    15421 gataaaaaag gtacaccata tgtagcgata atagctacta tgttaatcac tataccaata
    15481 gctctatctg gaagttttgc tgaattagca gcaataagtg cagttgctag attcatacaa
    15541 tacataccaa catctttatc cgtattagtg tttagaaaga aaagaccaga attagtaggt
    15601 acttttaaaa ctccttttgg agcagtgatt cctctaattg ctgtatgtgt tggaacatgg
    15661 ttgttattcc aagcatctat gcatcaatta attatgggat taggagcttt ggcaattgga
    15721 gtaccattat actttataat gaagtcttat aatagaaaag tctgtggtga aaatttaaag
    15781 aaaatagttt aattatgtta taagaattta ttgagttaaa attagacatt ggcagaaaat
    15841 taaatacaaa taagtatcta taaggctata ttaaaatatt attaattttt tattttaata
    15901 tagccttata ttgttatcaa taaatgaaaa atattgtaac aacagaagaa caatagtatt
    15961 atgtttatag ataaaaaact tattaggagg taaatttatg ccaacaataa cattaaaaaa
    16021 tggtgtagac gtacttatta gagaaggtgt aagagaagat gcttaaagca ttatagattt
    16081 ttataatgaa gtgggtggag aaactcattt tctgtcattt ggaaaggatg aatataaaat
    16141 atctttggag gaacaagaaa atgctataga atcagcaaaa gcatctgata attctgttaa
    16201 actgattgca tttatagatg atgaaatagt tgggattgca acgattgatt ctaatcaaaa
    16261 agcaaaagga aagcatgttg gagtattagg aattgttgta aaagaaaaat attggggaat
    16321 tggattgggt aaaaggctta tgttagacct tatagaatgg tgtaaatcaa atggaataac
    16381 caagaagata actcttgtca ctaatgaaga aaattataat gctataggat tatacaaaaa
    16441 agttggattt gaagtagaaa gcatactaaa aaaagaatgt tattataatg gtgtttatac
    16501 tgatttaata ggaatgtcac ttttattggg aatataatta gtgtacattt tatctcgaaa
    16561 aaagtatatt aagtatggta taattaaaaa agaatataac ttaatataat tttggaggac
    16621 gaattatgaa aaatctgtgt aaaatatcag atgattattt aaacagcaag cttaagtatt
    16681 taaagttatt atcaaaacaa tatccaagta tatcaaaagc aagtacagaa ataataaatt
    16741 tagaggctat attaaatctt ccaaagggaa cagagcattt tataacagat gtacatggtg
    16801 aatatgaacc atttgtacat gtattaaaaa atggttctgg tgtaataaag agaaaaatag
    16861 aagaattgtt ttcaaataca ataagagaca gcgaaaaaaa aatgttagca actcttgtat
    16921 attatcctga acaaaagtta gacctaataa ttaagcaaga ggaaaatata gatgattttt
    16981 atagaataaa tatttataga cttatagaac tttgtaaata tgcttctagc aaatatacta
    17041 ggtctaaagt tagaaaactg ttaccagaaa attttaaata cataatagaa gaacttttgc
    17101 atgagcatgt taaaagtgaa cataaagaag agtattataa aagtatagtt gaaacaatag
    17161 ttgacatagg aatagctaag gagtttataa ttgctatttc tacagttata caaaaattgg
    17221 ttgtagatag acttcatgta ataggagata tttatgatag aggtccaaga ccagatataa
    17281 ttgtcgataa gcttattgaa catcattgtg tggatattca atggggaaat catgatatat
    17341 tgtggatggg tgcagcctca ggtgaaaaaa cttgtatagc aaatgcactg aggatatcag
    17401 ctagatatgc gaatttagac attgtagaag acatatatgg aataaatctg ttgcctttag
    17461 ccacttttgc tatagaaatg tataaagatg acccttgtaa agaatttatt ccaaaggtta
    17521 atgaccaaag tgtaacaaca acagaaaagt ctttaatggc aaaaatgcac aaagcaataa
    17581 gtataattca atttaagctt gaaggtgagg ttataagacg aagacctgaa tttgaaatgg
    17641 aacatagact tttgcttaat atgataaact atgatgaagg tacgattact ttaaaaggta
    17701 aaacatataa attaaaagat acttatttac ctacaataga caaaaaagac ccatataaat
    17761 taactatgga agaaagaaat gttattgata aattagtatc ttcatttaga ggtagtgaaa
    17821 aattacaaaa acatgtttca tttttgtttt ctaaaggaag tatatattta aaggctaact
    17881 caaatttact gattcatgga tgtgtaccat taaatgaaga tggaagcttt atgtctatga
    17941 atataatggg aaaagagtat aaaggtaagg ctcttatgga taagatggag tctctagcaa
    18001 gagaaggatt tttctttaaa gataaggcag aagaaaaact atatggtatg gatattatgt
    18061 ggtatttatg gactgggaaa tgttcatcat tatttggtaa agatgatatg actacatttg
    18121 aaagatactt tatagaagaa aaagagactc ataaagaaaa taagaatcca tattttaaac
    18181 ttagagaaaa tgagatggct tgtaaaagat tatttgaaga atttgattta gagcttgatg
    18241 aatcccatat aataaatggt catgttcctg tggagagtaa aaatggagag agcccaataa
    18301 aagctaatgg taaaattctc gttatagatg gtggtttttc tagggcttac caaaaaacta
    18361 ctggtatagc tggatacact ttgatataca attctagaac tttgcaatta gtatcgcatg
    18421 aaccatttaa ttctgctgaa gaagccattg caaatgaaag tgatatctta tcgacaactg
    18481 ttgtagtaga gcataaggca aagagaaaaa tggtgagaga tactgatgaa ggagtaaaaa
    18541 tacaagagga aatagaagat ttaaaactac ttttaatggc ttataagaaa gggctaataa
    18601 aagaaatgta gatattatag aggcacttca taattgaagt gtcttcttgt taatacttat
    18661 taaatatatt ataaagatta ataatagtag tttatattat acttactaag tgaatccaag
    18721 ataaatgggt tcactttttt tatttataaa cattattaaa aaaatataga tgttttggtt
    18781 atattccttg tcctttataa atagaattat ataaacaaag gaggaggaca agagatgaat
    18841 aaactttctg atgaaataat aaactctctt tctacaacta taagagaaaa ggtagaaaaa
    18901 gtctcaaaca ataatctgaa catagaagaa attaggttac gctcacaaaa accattgata
    18961 ctaaatgcaa actctaaaga ttatttttac aatcaaaaaa ctatgacact tgatttaaat
    19021 caacaaaact catatgtagt aacaagagaa gatgtagagc aaacttttca aataatatgt
    19081 aaatattcaa tacattcatt tatggatgac ataaaaaaag gattcataac tttgagagga
    19141 ggtcatagag tagggttagt tggaaaagca atagtagaag atggacaagt caaaaatata
    19201 aagcatatat catctttaaa tataagggta tcaagggaaa ttattggatg ctcggataag
    19261 attttaagtc atattataaa gggaaaaaat cagattaata atactttgat aatatcacca
    19321 cctcaatgtg gaaaaacaac tttaataaga gatatagtaa gaaatttaag taatggtaat
    19381 gaagattatg gatttaaagg tttaaaagta gctttggtag atgagcgtaa tgagatagca
    19441 ggagcttatc taggtgttcc acaaatggat gtgggaataa gaactgatat aatagaaact
    19501 tgcccaaaag atttgggtat tacgatgctt ttaaggtcta tgtcaccaaa tgttattgtg
    19561 acagatgaaa ttgggagtga aaaagaaata aaggcattat acacagcatt aaatggaggt
    19621 ataggattaa taactactgt gcatggagat tcaatagatg atatacaaaa tagaaaagaa
    19681 ttaaatagac ttctggataa agagttattt aaaaaggtta taattctttc tgcaaaaaga
    19741 ggagcaggaa cgattgaaaa aatttatgat ttagaagaaa agagatggta ttttgcaaat
    19801 taaaataatt attatagctc tcttggtagg aagtagttat ttgataggtg aacaaatata
    19861 caaaacttat actcgtagac ataaacaatt aaacgattta ataagagttt tagaaatttt
    19921 gaggatggat ttatctttcg gactttatac attagaagaa atctttaata ggataggtgg
    19981 aaataaagag ttttgttttt ggaaattttt ctatcaaata tcagaaggac ttcacaatga
    20041 acaaagtaaa actttggaaa taataatatc agaaaatatt gatgtcttaa gtaaagaaac
    20101 ttatctaggc aataaagaaa ttgaggaact gaaaaatcta atacttactt tagggaaaag
    20161 cgatatagag tcacaacaaa gaatgataga tttatctata gaaaatttga aaaaacaaac
    20221 ctatgagaca aaggaagata taaacaaaaa aggcgtattg tataaaaaat tagtgacatt
    20281 cataggtata ggtatatgta ttatattgat ttaatatggg gaggtttatt atggaaatat
    20341 cattgatatt aaaggttgca ggtgtcggta ttctaatatc agtattgaat atgatattgg
    20401 aaaaaacaga tagaaaagat tgggcaggtc ttactacatt agcaggtgta ataatcgtat
    20461 tgggtatggt aataacagaa ataagtgact tgtttaatac tgtaagaaca atgtttcaac
    20521 tttactaaaa aggagggagt aatttggaaa taatgcaatt aataggaatt gcaatcattt
    20581 caactacact ctgtttagta attaaaaaag atagaccaga gatagcaaat ttcatagcaa
    20641 taataactgg agtaataata ctattgtcag ttatgtttaa gctaaatttt atagtagata
    20701 gtatacaaga tttggctaac aaggctaaca taccgactat gtatatatca ttaataataa
    20761 aattaattgg gatagcatac ttaatggaat ttgcgataca actttgtaaa gattgtgggg
    20821 aaggaaacat tgcttcaaaa ctagaatttg gaggtaaaat aatagttatg tcaatgtctt
    20881 tccctatact tttgtccatt gtagaaatgg tggtgaatat aattccatag atgtgtatct
    20941 tggaatgaat ataagtatat tagagaactt tcgttgtatt gaggtttata gagtgaagag
    21001 aggcatggtt gcttatgaaa aaaagatttt taccaatgat tataggtttt ttatttactt
    21061 tcttttttgt aaatatcttt gctattatga tatttgctaa tgaagtacca agtagtgaag
    21121 ataaagaata tggtgaaaca aaaaatagca tagataaata tatagatggt caattagaca
    21181 agctagatat aaatgagatt caagactata taaataaaga aatagttatt aatgatgtta
    21241 atttaaaatc atttgtaaaa gatttaatta gtggagaaaa aaatatctta gatttattca
    21301 ataaagatgg attgaagata ttgatgtttg atgaatttaa agcaagttta aaggtagtag
    21361 cagtaatttt ggtactggct ctgttatcat ctattttaaa gagtctggaa aactcttttt
    21421 catcaggtgc agtaagtcaa atagcaacat atataatatt tatcacaatg gtatcactta
    21481 cacttattgg ttttaaagat gtattacaga tttgttatga tgcaatagac catactgtag
    21541 gtcttatgca agttataatg ccaatattaa taacattttt acttttaatt gggttcccaa
    21601 taacgtcgac tactttgaat cctatcttca taggaggagt aacatttata aatgtatttt
    21661 ttaaaaattt tctatttgta tctattactg ttgcatttgg aattttaata ataaacaatt
    21721 tatcaaaaaa tataaggcta aagagatttt tctcctttgt aaaacaaata aattatgttt
    21781 ctattggagc tatgtttact gtttacttag gtcttgtatc catacaagga ttgtatgtaa
    21841 ccagttttga taaattcagt gtaaagactg ctaaatttgc tataggtaat tttattcctg
    21901 ttgtaggtgg atttgtatct gactctgtgg atattttatt atcttcatct cagcttataa
    21961 aaaatatatt tggaggaata gggcttatct tacttgttgg aatttgttta cttccagtaa
    22021 taaaaatttt atcagtaata gtagtgtaca aattagcagc aataattgta gagcctgttg
    22081 gagaggatgg aatatctaat tttttaaatg aagtggcaaa tttaatgata ataatgctag
    22141 catcagtaat tgctataaca gtaatgttct ttgtaactgt agctatcttg acctcgataa
    22201 gtgtcgtaag tcaagggtaa tttatatatc agtgaaaaat aattacattt aatctaatgt
    22261 ttttagagag gaggatgcta tgttagaggg cataaaggca tggatagtta gtgttttgat
    22321 aggagcattt atagtaaata tagtagatat gattttacca agttctaaga taaaacctta
    22381 tgtcaaccta gttttaaact ttatgtttgt atttattgta ataactccag tagtaggatt
    22441 tttttcaaaa gatatgagtt tagaagatag aatattaaaa tctatgggaa actacaacaa
    22501 gcagtatgta gatagtacaa atgctttggc aaaagagact ggaaataata gtctatccaa
    22561 gggatatgag gatgggttaa aagaagtact taaattaaaa cttgatgaat atggttatga
    22621 tttagaagat atagagctta atggggcaaa tataaataat ataaaaataa aagaaaagaa
    22681 taatagtgca aaaagtaata acagttccaa tgaagaaaat aaaaataata gtactaaaag
    22741 agaggaagaa aacataaact ctaatgacaa agaaaattct aaacaagtct tcaaaaaagg
    22801 tacagagtat gggttgaacc taaatgaaga aaaattaaaa aacgacttaa taaaggtgct
    22861 ggatgtttct atagaagata tacaaattga taaataggag ttgggagaat gttgaaaaac
    22921 ttaaatgaca aagataagag aaaggtatat tcactaataa ctatagcagg aatatgtgtg
    22981 gtttcattag tattattatc ttgttttcct agtagcaaaa gtgataaaca agttggaaaa
    23041 acagagacta ataaaaatgc tgaaaagcaa gtcactaaaa aacaagaaaa agatgattta
    23101 gagtcaaaat taacagcaat actatctaaa attgatggtg caggagacgt tgatgtaatg
    23161 gttacatttg aatcaagtga agaaatacaa cctgctttta attcaaataa tacaacagaa
    23221 acaacagagg aaaaagatgc acaaggtgga gaaagaacag ttacgacttc tagtgaaaat
    23281 aaaacaatga taacttcaaa ttcaagtgac cctgtagtta taaaaactac agagccaaag
    23341 ataaagggag tgatagtggt agcaagtgga gcaagtgacc caagtgttaa agagacactt
    23401 tatagtgcag tacaaacatc attacaagta gctggtcatc aagtagaaat atattctaaa
    23461 taaatacata taaccggggg gataaatgaa tgaagtttaa ttataaggga agaggatttg
    23521 taataataac tttaactgca atgttagtgg tagtaggaac agtgaattat caactaagta
    23581 agaaatcttt attagaaaca tcaaaagagt ttaaggcata tgaagaagca cagttgcaaa
    23641 aaaatactga tgatagtgat agtagtaaga cagaggattc tagtaatgta gataaacaag
    23701 gtggtaagga aagtgctgat attgatatag tcgatagcaa agctagtaaa gtaaaagaaa
    23761 aggcaactga gacaagtaag gaaataaaag ctcaattatc atcagagaaa aatatgaaaa
    23821 aggcatctta tattttagat atgaaaatga atagagaaaa acaaagaaat gagttagttc
    23881 aagatttaaa tgaaatgata aataatccat ctacaacaga agaatctaga aaagaagctt
    23941 ctaatatgaa attaaatata gttaaaattc aagaaaaaga acttcaaata gaaaaccttt
    24001 tgagtacaaa aggatatgaa gaagcgttag tttatataag cgataacaaa gttaatgtag
    24061 ttgtaaatga agctaaatta gaaaagaaag atgcagcaaa aatatttgat ttagttgcag
    24121 aacaagccaa tgtgaagtat gaaaatatta aacttacaaa taataatagt aataagtaaa
    24181 gtataaacct cagaattaac tgaggttatt ttcttgcaaa gtagacttgt atttggtatt
    24241 attatactta taaaaatagt tccttaaaat caggaggtaa gttttaatat ggaagataat
    24301 aaatttggac aagtaaaaat atctaatgat gtaatagcta caatagctgg tttagcagca
    24361 ttagaagttg aaggcataga aactacagca acattaacag ataagttgtt aaaaaataat
    24421 ggagtaaaaa tacaaatcga agaagaggat gttaatttag atgtaatggt tacgataaag
    24481 tatggaatgt caataccaga tacagctttt aaagttcaag aaaatgtaaa aaatactgtt
    24541 gagacaatga caggactgaa ggtttctcaa gtaaatatac acattcaagg aatcagcttt
    24601 aagaaggata aagttgataa agaagaagca aaagcaacta agaaaaatta atattagccc
    24661 tctgaaaagt cagagggttt tttgctggta attgatttta tatggataat taaacttaaa
    24721 ggagaatttt gattgatgaa aaaagatagg gcacaaaaga gtacaacaag agaatatata
    24781 atgaaactta tataccaaat aaatattaat aaagaagatt ttgaaacttt agaagataaa
    24841 gtagataatt ttttaaaaga taattcagaa catattataa atagatataa agaacttgca
    24901 cttcaatact caaaaaatac aaatttaaag ttagaagata ctgaaattga agatgttata
    24961 gacaagaaat atataaatac agtatgcaaa gcattaaaag aaaatcatga caaaatagat
    25021 gagttaataa ataagcatgc taaaaactgg acagtagata gaatgccaaa ggtagatgtt
    25081 tctatactta gattgtctgt gtgtgaaata ctttatttag acactcctaa taaagtatca
    25141 ataaatgaag cggtagaact tgcaaaaata tattgtgatg ataagtctcc taaatttata
    25201 aatggtatat tgggaagtgt tgttgatgaa attgggaaat aatataataa ttggaattga
    25261 tactagctgc tatactactt ctatagcagc tatctcttta gataagaaag ttatattcaa
    25321 tgaaaaaata atgttagaag taagggataa ttcaaaaggt ttaaggcaaa gtgaagctgt
    25381 atttcaacat atcaataatt tagggattct tagtgatagg ataaaatctt ttaaagataa
    25441 gtttaatgta gaaggagttt gctcatcaaa aaaaccgaga cctgttgaaa actcatatat
    25501 gcctgtattc aatgtaggtc ataattttgg aaaattattg tctagtatat atggttgtag
    25561 gttttatgag actactcatc aagagaatca tattgaagca agtttattaa atagtaaatt
    25621 aaaaaataat aataaattta tttctgttca tatgtctggt ggtactacag agatactttt
    25681 aacaagcaaa caagatagtc atcacaatgt atgtgatact aatttaggta aaattgccaa
    25741 aataagtatt aaaaaagatg ataaaagtaa gttatacaat aattttggat ataatataga
    25801 tataattgga ggaagtaaag atattagctt tggtcaactc atagatagag taggaattaa
    25861 gcttggatat aaatttcctt caggaaagta tttggatgaa aatgctttaa attgtaatct
    25921 taaaatagaa agtggtctga aaacttctgt aagagatggg tatatgaatt tatctggatt
    25981 agaaaatcag gtaaataaga ttataaatga taatggagat aatactaatc aaaaagaata
    26041 catatctaaa ttagtattag attcagtagt tagaaatatg tttaaatctt tagtatattt
    26101 atgtgaaact tataatgtaa atgaagtagt atttgctggt ggagtttctg caagtaagta
    26161 tattcttaga gaattaagca tgaagttaag aaaaaagcat atagaagctt attttacaga
    26221 accacaatat tcaactgata atgctgtcgg gtgtgctata ataggattga ataacttttt
    26281 aggagaaaga gtatgaaact aagagcttta gatataagtg aagcaaactc atatattaaa
    26341 agaattttaa taaacgaccc aatactttct aatcttaaag ttaaaggaga aatttctaat
    26401 tttaaggttc atagtagtgg gaatgtatat ttatcactta aagatgaaac atcaaagtta
    26461 aactgtgtaa tttttaaaag taattttaat cgcaatttaa aattagataa tggtgtaaaa
    26521 attatagcta atggatatat atcagtctat gaaagagatg gtgcgtatca attatacatt
    26581 aatgaaattg aaatagaggg aataggaaat ttacacatag agtttaatag attaaaggaa
    26641 aaacttaata aagaaggatt atttgaccct aaatacaaga tacctatacc taaaatgcca
    26701 aactcaattg gcgttataac atctccaaca ggtgctgtta taagggatat aataaatgta
    26761 ataaaaagaa gatatccgaa agttaatata aagctttatc cagtaatggt tcaaggagat
    26821 aaatcagctg aagaaatctg tgaagcaatt agatttttca atcatatgaa gaatgttgat
    26881 acacttatag ttggtagagg tgggggttca atagaagaac tttggtcttt taatgatgaa
    26941 atggtagcaa gagaggtttt taattctcaa attccgataa tatcagcagt tgggcatgaa
    27001 acggatttta ctatttgtga ctttgtgtct gatatgagag ccccaacgcc atcagcagca
    27061 gctgagattg ctactccatc attggatgat ataaactata aacttggaaa cataaaaagt
    27121 aggatgagta agtctcttac aaatcaaata gagctagacc aatataggtt agaaacagta
    27181 tttaataaga taaataacta tttagattcg tacacaataa aagataaagt tatacaatta
    27241 gacaaaatat atgataaaat aatttttgga atagaaaata atttaaaatt agaagatgaa
    27301 aaattagtaa aaattggagc attactccat aatttaagtc cattagctac tatggataga
    27361 ggttatagta taactcaaaa aaatggaaaa gtaataaata gtataaaagg attaaaaata
    27421 aaggatagta tagatatagt attaaaagat ggaaatttag aatgtatgat agataaaatt
    27481 gaaaataaag agggatagta tgaatttaac ctacgaagaa gcttataaaa gattagaaag
    27541 tattttaagt gaattagaat ctaaaaatgc tagcttggat gaatctttaa gcctatatga
    27601 agaaggtata agcctttata aacattgtaa taagctttta gatgatgcta aattgaaaat
    27661 aagtaaattt aatcaattag gaatagaaga agactttaaa atagaggagg aataaaattg
    27721 gaatttaaac aatgtctaaa agaaaaagca agttttgtag aaaaagtact taaagaatat
    27781 atgcctaaag aagaagggta tcaaaaaact gttattgaag ctatgaacta tagtttaagt
    27841 gctggaggta aaagattaag accaatactt acattggagg cttgtaaaat agttggagga
    27901 aatgaagatg aagctattcc ttttgctatt gcaattgaaa tgatacatac atattcacta
    27961 atacatgatg atttgccagc tcttgacaat gatgatttaa gaagaggaag accaacaaat
    28021 cataaagtat atggagaagc tatggggatt ttagcaggag atgctttgtt aaactatgct
    28081 tttgaagtaa tgcttgcagg ctcaatcaat aaagaaaatc cagaaaagta tctaaaagct
    28141 ataaatgaaa ttgccaaagg tgctggtata tatggaatga taggtggtca agtagtagat
    28201 gtagaaagcg aaaataaaca aattgaaaaa gaaaaattag attatataca tatgaataaa
    28261 actgctgcaa tgatggtagg ctgtatgaga gcaggagcta ctataggtgg agcaaattct
    28321 gaacaaatgg aagaaataac taaatatgca aaaaatatcg gattatcatt ccagatagta
    28381 gatgatatac tagacatagt aggagatgaa gctaagttag gtaaaaaggt tggaagtgat
    28441 atagaaaatc ataagtcaac atacccatct cttttaggtt tagataaatc taaagaaatt
    28501 gcacataatt tgattgatga agcaaagaaa agtatagaaa aattatctga tgatgtagat
    28561 tttttaaagg gattggctga atatataata gatagagaat actaaaatat acagctaaaa
    28621 tcgaggaggg aaaaatggat tttttttcgg agatttttaa caatggggct ttgggaataa
    28681 gtctgattgc ttgtttctta gcacaattta taaaaatatt tactggtaag gaaaaaagaa
    28741 ttgagttatc aagaatactt atttcaggag gtatgcctag ttcccatagt tcttttgtta
    28801 caagtttagc tacagtggta ggtattgaaa aagggtttaa ttctactgat tttgctataa
    28861 ttaccgtact tgccttaatt ataatgtatg atgcagcagg tgttagacga gctgttggta
    28921 aacaagctac tatattaaat caaatggtgg cagatataca gcatggaaag catatagagc
    28981 agaagaaatt aaaagaatta ataggtcata caccactaga agtttggttt ggagctttac
    29041 ttggaatagt aacagcttta atattgatgt aaattgaaaa tttacagaat aaaggtgagg
    29101 ataatatgta taaatattta gataaagtaa attctccaaa ggatataaaa aatatgagta
    29161 tagaagaaat ggatttactt gcaaaggata taagaaagtt cttagtaaaa tctgtttcaa
    29221 agacaggagg acacttagct tctaatctag gagtggttga gttaacatta gcacttcata
    29281 aagtatttga tagccctaaa gataagattg tatgggatgt agggcatcaa tcttatgttc
    29341 ataaaatagt tactggaaga aaagattgtt ttgtatcttt gagacagttt aatggactaa
    29401 gtggttttcc aaaggagaat gaaagtcctc atgatatttt tgatacagga catagcagta
    29461 catctatttc tatagctaca ggtattgcct gcgctaggga tataaaaaaa gaaaactata
    29521 gtgttatatc tgtgatagga gatggttcta taacaggagg tatggcactt gaagctttaa
    29581 atcaattagg atatatagat accaatatga tagtaatcct taatgataat gagatgtcca
    29641 tagataaaaa tgttggagga atgtctaaat atctgtcaag tattataaga aactcaacag
    29701 ttgagaaaat gacagatgaa gttgataaaa ttttaaatgt tacacaaaca ggtgaaattt
    29761 tatctaagac tgcacataga tttaaagata aactaatgta tagcttttct cctcaggact
    29821 gttcattttt tgattcatta ggtataagat attatggacc tatagatggc cataatacta
    29881 aagaactaat agatatattg agaaaagcta aacacaaaaa agggcctgtt cttttacatg
    29941 taattacgaa aaaaggaaaa gggtacagat ttgcagaaga acaacctgat aaatatcatg
    30001 gagtatcaaa gtttgatata aagacaggtg ttacatctgc aaaagttaag tctatgtcaa
    30061 ttagtgttgg agaaaaactt gttgatatgg ctaataataa tgaagatata gtagcaataa
    30121 cagctgcaat gccatctggt acagggttaa acctgtttga aagtgcatat ccaaaaagat
    30181 attatgatgt tggtatagct gaacaacatg ccacaggatt tgcagcaggt cttgcaaaaa
    30241 atggtatgaa accttacttt gctgtgtact catcattttt gcaaagggca tatgaccaag
    30301 ttattcatga tgtatgcata acaaaaaagc cagttacttt tcttatagat agagctggat
    30361 tagttggaaa tgatggagaa actcatcatg gtatgtttga tttaagttac ttaaattcta
    30421 ttccaaatat tgtagtaatg gctccaaaag acacaagaga gatggagctt atgatggatt
    30481 tgtcattaaa attagattgt ccactagcaa ttagataccc aagaggaagt agttattacc
    30541 tagataaggg agaatatgga gagattgtac tgggtaaata tgaggtatta gatgacggtc
    30601 aagatacagt catactttgt attggaagta tggtaaaaca tgctttagag gctaaagaaa
    30661 tattatcaag agaaggaata aatccgacaa tagttaatgc tagattctta aagccaattg
    30721 atgaaggtat gttaaaagca ttattaaaaa atcataaaaa tgtagttact atagaggata
    30781 atattgtaac tggaggattt ggaagtagaa taaacaagtt tattatagat aatgaatata
    30841 acgtaaatat acttaatata gcaataccag aagaatttgt aaaacatggc aatatagatg
    30901 aattatatga ttttgtagga ttatcgccaa aaagtattgc tgataaaata aggaaattag
    30961 ttattgagta aaaatctaat gtagatattg taaattaaaa tttatataaa atatagaatt
    31021 tatcaattag aataaatatc aagtgtgtta cttcaagcta ctttagttat cattaaagta
    31081 gcttttaaca gaaaggtaat tgtatgaaaa aaagaataga tttattatta gtagaacaag
    31141 ggtactttga aagtagagaa agagccaaaa aagcaataat ggctggattg gtttttgttg
    31201 ataatcaaag gtgtgataag gctggtacag aagtaaaaga agattgtagt atagaagtaa
    31261 aaggaaatcc aataccatat gtcagtagag gtggtttaaa gcttgaaaaa gcaatgaaaa
    31321 actttgattt gactattgat ggaaaggtat gtatggatat aggggcatcc acaggtggtt
    31381 ttacagattg tatgctcaaa aatggagcaa taaaagtatt ttctatagat gttggttatg
    31441 gtcaacttgc atggaaattg agacaagatg atagggttgt gtgtatggaa agaactaata
    31501 taagaaatgt tacaatagaa tatacaaagc agtttgctga ttttgcgtca attgatgtgt
    31561 cttttatatc gttaaagtta gtacttccaa aggcaaagga gttagtacgt catgatgggg
    31621 aagttgtcgc tttaattaaa cctcaatttg aagcaggaag agaaaaagta ggtaaaaaag
    31681 gagtagttag agaaaaatct acacatatag aagttataaa aatgatttct gatttctcag
    31741 tcgaaaatgg atttgagatt ttagggttag atttttctcc tattaaaggt cctgaaggta
    31801 atatagaata tctaattcat ttgagaaatg gaaatgaagg atatgaattt gatggtgaga
    31861 catacaataa caaaatagta gaagttgttg aagcttctca taatttagac aaataggtta
    31921 aaagggggct tagatttgat cctcgaatta tatatgaaaa attgtgcctt agttgaagaa
    31981 ctaaggttga atattgataa aaacttaaat atacttacag gtgaaactgg ttcaggaaaa
    32041 tctataataa tagatgcact tggactttgt ttaggtgata aatatgatag gtctttttta
    32101 agaaaaggaa cagataaggg acttgttgag gctgtatttt tttcagacaa tagatatctt
    32161 aaaaaaatat tagaagaaaa tgatataagt atggaagatg ataatttatt ggtaataact
    32221 aggttgatat actcagatgg taagagtact gctagagtaa atggaaggac tgttaaagtg
    32281 tctcttctta aagaaattgc atctacactt atagatatac atggtcaaca tcaaaatcaa
    32341 gctttgttca ataaagatac tcatttaaaa tttctagacc tttttggaga aaatgaattg
    32401 gaagagttta aaattgctta taaaaatgtg taccataagt atagtgaagt aaaaaaagct
    32461 ttaaattgct tgacagaaaa taaagatgag atgcaaattc aacgagaaat agatttatta
    32521 agatttcaaa taaatgaaat tgaagctgca aatctaaata agaatgaata tgaagattta
    32581 ttaaaacaaa gagaagttta tagaaatagt gaaaagatat ataataatct aaattcgagt
    32641 tatagcaagt tacataatgg tgagtataat gttattgacc taataggtct tgcatctaag
    32701 gaacttaatg acatatctaa atatgacagt gttttaagtg aatatagtga tactgtagaa
    32761 agaattatgt atgagttaca agacatatct ggtgagataa gaaattacaa agataatata
    32821 gactttgaac cttatgagct agagcagatt gagcttagaa tcgacgaaat taataattta
    32881 agaagaaaat atggagacag tatagatgat atatttgagt attatggaaa aattaaagat
    32941 agattagatg aaatattaaa tagagatgaa agagttgaac agttaaaaag caaactgatg
    33001 aatatagaag aagatttaaa aataaaagcc agtaaattga ctaaagcaag aatagaagta
    33061 gcaactactt tagaaaaaat acttcttgat gaattaaaaa gcttaaatat gaaaaatgta
    33121 atgttcaagg ttaattttga aaaaggttca tttactttag atggagtaga tgatattgaa
    33181 tttatgatat cttttaactt aggagaagat attaagccta tatataaagt tgcatcaggt
    33241 ggtgagatgt ccagatttat gttagcgttt aaaaccatat tggctgatat agatgatatt
    33301 gatacattag tgtttgatga gatagatact ggaataagtg ggatagcagc acagatagtt
    33361 ggtgagaaat tgagtgatat agcaaagaaa aaacagataa tatgtataac tcatttacct
    33421 caaattgctg caaatgcaga cactcattat tgtatagaga aagatacttc aaataatagg
    33481 acgtttacca atgttagtaa gttaaatgaa tctcaacgaa aaaatgaaat tgccagactt
    33541 atagcaggta acaatataac agaaaaaact attgaacatg caagtgaaat cattgaattg
    33601 gctaaaaaat gttagtgaaa aacaagcatt ttaataggtg taaagaattt ttatagaaat
    33661 gctttacacc tattttttat gatgaaatta ttttaaaata tctagtgcct atattgaaga
    33721 aaagtaggtt tgaaaatgga atgaactttg tataaatttg taactttgaa atttctaaat
    33781 gcttaagtat aatattattt attaaggtag tagtgagggg aggcataaaa tgaataaaaa
    33841 agatactatg cacttaattt ttataattgt aatttttatt ttattatggt atattgtggt
    33901 agcagtaagt gagattaata aaaatattga aagttgtagt aacaaattag attatttgtc
    33961 acaagagctt atgagtacta agtcggatat tagtaatact ataaatgaag aaatgtcaaa
    34021 aagttatata acaaaaagta ttgacttaaa agtaaaaaaa atcgagaaaa aagaatgtac
    34081 tattgatgta gatgttcaat taagtaaact tggaaaaaat ggaaaagtat tttttatgta
    34141 taaagaagat agtgataaat ggaatgaaac tgagatgaca aaacatgggg aattatcata
    34201 tgcatgtgaa ataaagataa atacaggaag tgaatatgat tataaagtag ttacaagtgg
    34261 agatatatct gaaagttctg atgtacaaaa actgactaaa tctgattata tgcctgaaca
    34321 acctatattt aattctggga taagagataa tagagaatac tttattgaaa tagtagaaaa
    34381 tagtaatctt tttaatcatg agagtgaaaa gtcaaaaaat aaagcttacg ataatatcag
    34441 gttagaaaaa gtagatatta ttgtaaatga aggaaaaaaa gacactatat acaaagccca
    34501 attagctgaa ggcttagatg atggcaaaac taataattca aatagaaatc aagtgaatta
    34561 tgaagtaagt tttcctaaaa gggatattga tgacataatt tatattaaag ctaaacttac
    34621 atacaataat ggagtagaat atacaaagga tataactgaa gatataacta tgggattaca
    34681 agatttagaa aaataaatac atatttaaat aaatattcaa atagatttgt tcacaaatag
    34741 agtataaata aaactgtagt agttttactt atactctatt aaaaaattat aattttaatt
    34801 ccatatatct agccccttca attggattat aagtataaag ttctatatca taaaaaccaa
    34861 acttttcata taatccttgt gcacttttca tgcttggaag agtatctaat ctcatgtgtg
    34921 tatatccaat ttttttagct tctacaatga tttcttccaa tagtattttt cctattttta
    34981 atcctctaaa ttgatttctt acatataatc tttttaattc acatatttta ccttcaagtt
    35041 tttttaaagc aacacaacct gccaaatttt catcaacaaa tgccaatatc aatgagcctg
    35101 aaggtttttt atattttcct ggaagtgttt ttaattcatt attaaagtct tgaaaacata
    35161 agtctatatt tagagaattt gaatattcag taaatagaag ttttacgttt tcaagatttt
    35221 ctaaatcttt ttcttcaaca aattgtaaag ttatattttg agccatttta tccgccatta
    35281 tataaacatg tttatataat gacttcacct ccttgatata tgtatatttt tcttaatatc
    35341 aattagattt taataccatt actttttcac caccatagtc aagctctcca ttaaacttaa
    35401 atccaaaatc tttataaagc ttaatagcca ttttattgtc ttcgaatata ctaagatata
    35461 gttcatcaca gttatattca tttttaagat gatttacaag aaaatctaat gatgcttttc
    35521 cacatccttt accttgatat tttgaatcaa tcataaatct atcaagccaa actctatcat
    35581 cttctaaacg accatacata gcaaatccta ttaaagaatt ttcatcatat attccaacag
    35641 gtacccaagc tgttataaat ttagattcag caatagaaag tgcattgctt tctatgtaat
    35701 ctaactgact tttatctaca gacaaactaa ctaaatcgtg ccaattattt atgtctgcaa
    35761 ttcttacatt taatttcaag atattaccgt ccttcaaact ttttgttaca atctaatact
    35821 atcatattat gtattaaaaa tcacttatta agattttaaa taaacttttt gaatgaataa
    35881 attctcagga aaaaggaatt ataaatacat aataatacag cataggagta gatggatata
    35941 tgaacaaaat aaataaaaat ttcaaaaata tttggataat attaggagct gtatcgattt
    36001 ttaccatggt tataattttt aataaaaatt gtaatgttaa taaagagact caaatagaag
    36061 cttttacaat taagaatcat gataaaaaat ttgtatatcc tatgggaaat ataattggag
    36121 taaaagcaaa tacagatggt gtattggtct taggatatga ggaagaggat gtaggctata
    36181 ttggaggcat acaaataggt gataatatag taaaaataga taataaaagg ataaaaaata
    36241 gtcaagatgt atcagagatt ttgaataaga ttaaaaaaag taaagttgaa gtgacttttg
    36301 agagaaaaaa taagtataaa acagaagtaa tagagactaa aaaagaaaat ggcaagtata
    36361 aacttggatt atgggtaaga gataaaatat ctggaattgg aactatgact ttttacgacc
    36421 ctagtatgga aaagttcaaa gcaatcggtc atgccataaa agattcggat actaatgaac
    36481 tattaaagat aaagcaaggc tatatatata agccagaaca attaaaaata gtaaaagcta
    36541 gtaatgaaaa agtaggtaaa ataaaaggag attttaatga cagcaattta atgggtaatt
    36601 tctcaaataa ttcagagcta ggtataagtg gtaatattac agaaaaccat aataaagagt
    36661 ttaatgtagc aaataaagaa aagcaattaa tagaagttgg aagaccgcaa gatgtaaaaa
    36721 taggagatgc agtaattctt tttgaggata aaaacaaaaa tataacaagc tatgatataa
    36781 aaatagaaag tatagtatat gataaaggaa attatagaga tatggtaata aaagtagtag
    36841 atgacaagtt attggaatac acaggaggta tcgtacaggg gatgagtgga gctccaataa
    36901 tacaaaataa taaaattatt ggtgcaataa ctcatgtttt tagagataat ccgaaaaaag
    36961 gatatggtat ttttatagat gaaatgataa aattgtaggt gaggcattaa aaaattttat
    37021 tattttatca attatctagg aggaatataa ttttggagtg tcgaatatgc tttagagtag
    37081 ataattagga agcaattgtg taaaaagttt agttttctgt aataagaaga tgttttttaa
    37141 tggggggatt tttagtggaa aaaatcaaaa tagttttagc agatgacaat aaggattttt
    37201 gtcaggtatt aaaagagtat ttgtctaatg aagatgatat cgatatatta ggcatagcta
    37261 aggatggaat tgaagcatta gacttagtaa aaaagacaca accggactta ttaatactag
    37321 atgtaataat gccacatcta gatggattag gtgtaattga aaaattgaat actatggata
    37381 tacctaaaat gccaaaaata atagtactat cagcagtagg tcaagataag ataactcaaa
    37441 gcgcaataaa tctaggagca gattactaca tagtaaagcc atttgatttt gttgtgttta
    37501 taaacagaat tagagagcta gtttctaata gagttacaca agttgagcca aaacctagac
    37561 cagttcaaga gactcaaatg actagaagtg attttgttaa gaatgtagga aatatagaga
    37621 cagaaatcac aaatataata catgaaatag gagtaccagc tcatataaaa ggatatttat
    37681 atttaagaga agcaataaaa atggttattg ataatgttga gcttttaggt gcagtaacaa
    37741 aagagttata tccaagtata gctaaaaaat tcaatacaac accaagtaga gttgaaagag
    37801 caataagaca tgcaatagag gttgcatgga gtagaggaaa agttgacaca ataaatcaat
    37861 tatttggata tacggtacac aatactaaag gaaaaccaac taattcagaa tttatagcaa
    37921 tgattgctga taaattaaga ctagaacata gtatggttaa ataaacaaga cataaaaagt
    37981 aaggcttttt aattaaggca ttggctataa atgcgtatta caagcagcga aacggtataa
    38041 ccactagggt tataccgttt cgctatttta ataaatataa aaattttctt tattatttgc
    38101 ttactatatc aatataataa ttttattata ctatggatat agtatgtgtc tttacaagtt
    38161 gtaaactgac agtggtttat tttttaatat aaatattgac tttgatgcag gtaaactttg
    38221 tatttttaag cgtattatgg aatatgttaa ataaaaaaat gatgaaatat agtattgtaa
    38281 atgccaaaga tgcaaaacaa acttaaaaca tttattttat tgttaagtaa tgctataata
    38341 taatgtgatt ttaataatga tagtggaggt ttaaatatga gagtcgaggc ccctataaaa
    38401 gtagatcgaa aaaccaaaaa acttgctaaa agagttgaaa gtggggaaat agcagttata
    38461 aatcatatag acatagatga agttgctgca aactctttag tagaagctaa aataaaactt
    38521 gtcataaatg cggctccttc tataagtggt aggtatccca ataaaggtcc agggatatta
    38581 actgaaaata atatactaat aattgataat gttggagaag agttatttga agaattaaaa
    38641 gaaggagaaa ctatagaagt tgtagatgga aagatatata gaaaaggaaa atttttaggt
    38701 gcaggagaag ttttagacaa gtatgaggtt tcacaccaaa taaaggctgc ctatgagaat
    38761 ttagctgtag aattggatag atttatagat aatactatag attatgctaa aaaagaaaaa
    38821 ggatttatac ttggagaagt agaaattcca aaagtgaaaa ctaattatgc taataaacat
    38881 gtattgatag ttgtaagagg tcaggactac aaagaagatt taagtactat gctttcttat
    38941 atagaagagg taaagccagt attggttggt gttgatggag gagcagacgc tttaattgag
    39001 tttggatata ctccagatgt gattgtaggt gatatggata gtgtaagtga tgaagcttta
    39061 aaaaaggcta gtgaaatagt agtacatgct tatacagatg gaagagcacc aggtcttaag
    39121 agggttgagg aattaggctt agatgcagtt gtatttcctg cgccaggaac tagtgaagat
    39181 atagctatgc ttatcgccta tgagtataag gctgagttaa tagtagcagt tggtacacat
    39241 tcaaatatga ttgattttct tgaaaaagga agaaaaggta tggcaagtac ttttttagtt
    39301 agactaaaaa taggttcaaa gcttatagat gctaaaggcg ttaatttgtt atacagaagt
    39361 aaattaaaaa taaagtacat atgggcatta atagcaacag cattatttcc tgtgttggta
    39421 gttgcatcat tgtcaccagg agttcaacag ttcatacaac taatgcaatt aaagtttaga
    39481 gtattattac agatgtaaat tttaggagga taaatatgca tattaatatg aaatattata
    39541 tagtcactat aggggctata tttatagctt tgggtatagg catgttagta ggatttaatt
    39601 taaataataa tcaacaatta agtgaacaac aagcaaacat aataaatgat ttggatgata
    39661 agtttaatat cttaaaagaa aaaaatgata aacttgacag tgacttagca agtgtaaata
    39721 gagattatga agaggcagtt aattttataa ataaaaatgt agataagatt ttagctggtt
    39781 cgctgaatgg taaaagtata ggaataatat cggctaatga aaatgacgat tatacaaaaa
    39841 atatagaaga cataataaat aaatctaatg gaagtattgc atttaatata gtattaaaag
    39901 aaaatataac aaatccagaa aagttaaagg aaatgtcaac taaactaggt acagatgtaa
    39961 aaaatgcaaa tgatgcagtt aattatataa ttgacaccct taaaaaggaa gatgcaagtg
    40021 acattttaac atatcttcaa gaactagatg taataaagtt taactctctt ggaaatacat
    40081 atctaaaata tgattctgtt gtaatagctg gtggaaatga tgcaaaagac agtacaaaac
    40141 agtttgaaaa aatagaaaaa tttgtagttt caaaacttaa atcagagaat aaatatttag
    40201 tagaagttca aaatacagga gttaaaactt cttatgtaga actatattct aaaaacaaag
    40261 ttgcaactat agacaatatt gatgaaggta taggaagtat atctctagct attcttctac
    40321 aacaaggtaa tatagttggt aattttggta gattggatac tgcaacttca ttgttgccat
    40381 ctattaaata gaaataattt taaggagaga aaaaatggat tcttatataa gtataataat
    40441 accagcttat aatgaagaaa gtagaataaa atatacatta gaaagcatat tggacattaa
    40501 gcaaataaat gaaataatag ttgtggatga tggttcaatt gacaatactg ctaaggttgt
    40561 atctgagcta gaaaatgaaa agattaaatt ttttaagtta gataaaaata gaggtaaagg
    40621 atatgcttta aattatggta tcaaattagc aatgaaaaat gcagatatta taggttttct
    40681 ggatggagat ttaggaagct ctgctaaaga agttgaaaaa ttaatagtac ctatattaaa
    40741 tggtgaggcg gatgttacaa tagccaagtt tcctcctgca aagagaaaag gaggtttggg
    40801 atttgtaaaa ggtttagcta aaaattctgt gtttgagatg actggtgttg agttagatgc
    40861 aactttatca ggtcaaagaa tatttaaaag agaagtttta gaaaagtttg atgaaatacc
    40921 ttttggatat ggagtagaag ttggaatgac cattgatata ttaaagtatg gatttaaaat
    40981 taaagaagta ttagtaaata tgactcataa tgaaacagga agaaatttaa aaggttttat
    41041 tcatagaggg aaacagtatt atcacataaa aaaggtttta ggacaaaaaa agaaagaatg
    41101 gaggtaatat cttagtgaaa gattatatac tttataccat tttattatta actggattat
    41161 ttggaaccta tgctgtaatt ccactattta aaaatttatt gataaatgga aatgtactta
    41221 gaccaaacta taaaaaagat atgataccag taagtatggg aattgtattt ttgcctatga
    41281 ttgtaatcaa tggaataata cttgctttct ttactacaga atttaaggat ttagcgtata
    41341 tatttatgtt tatgtttggt atgatatcca tgttttttgc agggatacta gatgatgtca
    41401 ttggaaatag agatgtaagt ggtttaaagg ggcattttaa aagtttattt aaaggaagtc
    41461 ttacaacagg aggttttaaa gctttatttg gtggatttgt agggcttata gtttctgttg
    41521 ctatatcaaa agacataatt gatataatag taaatacact aataatagct ctatcaacta
    41581 atttaatgaa tcttttggat ttaagaccag gaagggcaat aaaagcatat ttattcataa
    41641 tggtaattat atttttaaca ttgacaggat ttgttcaagt tcttccatta cttatagtac
    41701 caaatgtact tgcatacttt aattatgatt taaaagcaag ggctatgatg ggagatactg
    41761 gttcaaacgt ccttggaata tctattggaa tgctcatttc ttttgggtat ccatttaaca
    41821 ttagattagg atggttaata tttgtagtta ttatgcatat attcactgag aaatactcat
    41881 taactaagat aatagaaaaa aataaactat taaattttat agataaattg ggaaggtagt
    41941 attatgataa gcaaaagagt ggggattgtt gaatcaatag taagtcaaac tgaaactcta
    42001 gatgatataa gagtaaatat aaatggaaaa atacaaaggg catataatta tcccaaaata
    42061 tctggaacta taaatatagg tgatgaagtg gttttaaata caacagcagt agaactgagc
    42121 ttaggtacag gtggatacca ttttgtaata actaatttaa ataatataga atctacttta
    42181 actgaaggtg ggcatataat gaaacttaga tatacacctc ttcaaataaa agtggattct
    42241 gttgaggaac aagaaagtat ttatcatgat aaatttgcga attttaaagg attagaaggt
    42301 ctttctgttg tagtagggac tcttcatagt atgttaactc catttgcagc ttcatttaaa
    42361 agaaataatc caaataaaaa attagtatat ataatgactg atggagcatc tcttccaatt
    42421 tatttaagta aaaatgtgga cactttaaaa gaaaaaaaat taatagatag tacgataact
    42481 ataggaaatg cgtttggtgg agattatgaa tgtataaata tatatactgc attaattaca
    42541 gcaaaagaaa tactaaaagc ggatgcagta tttgtcagta tgggacctgg aattgctggg
    42601 acaggaacaa aatatggatt tacagggatt gaacaaggct ctattcttga tgccgtaaaa
    42661 aaacttgaag gaagagcaat tgcaattcca agaattagtt ttgctgacaa acgagaaaga
    42721 catcaaggta tttcacatca tagtatgact gtatttaaag aaattgtaaa tgtaaatgta
    42781 gacataccaa taactatata tgatgatgaa aaattgaact ttataaaaga gcaaatcaat
    42841 ttaaatggtt tagacaaaaa gcataatata atttatatag ataataataa aacgaaagag
    42901 gatttagact attttaatct aaaagtaaag agtatgggaa gaaattatga acaagatgag
    42961 gcatttttca aagctgcaag cacagcagca tattacttaa tggaggttta agatggtatt
    43021 agaagaaaaa actattagta gtgatagggt ttatacaggt aaagtaatta ctttaaaggt
    43081 tgatactgtt gaaattccag ggcaaggata tcaaaaaagg gagttagtag aagttggtgg
    43141 agcagtagga atagttgcaa tcacagatga taataaagtt gtactagtta agcaatttag
    43201 aaaacctata gaaaagccaa tctttgaaat tccagctggt aaactagaaa aaaatgaaag
    43261 tccaaaagaa tgtgctgaaa gagaattaaa agaagaaact gggtatagtg caaaaaatat
    43321 taagctaata cataaatttt tcacttcagc aggtttttct aatgagataa tgtttgtata
    43381 tttagcaaca ggtcttactc caggagagaa taatttagat gcagatgagt ttttagatgt
    43441 ctatgaaatt gagcttgaag aagcatataa catggtatta aaaaatgacg ttgaagatgc
    43501 aaaaacatct attggattat tattagtaaa agatatgttt aaaaattaat atttataaaa
    43561 tgaataaccc tttgtcctaa agcatattat tttataaagg acaaggggga attaaattga
    43621 gaagaacata tagaaaaaat gattttagag aattaaatag acaaataatt attataggat
    43681 tgttgtttat gatgtctatt gttgttgggt cttacataaa taaaatattg ccaggttcta
    43741 gtaacaatat attaaacaat ataaatcctg ctgtagagta ttataatcta aatataagca
    43801 taaaagatac agtgatacaa aatttaaaat cagatgcaat ctttatgggc tcaatagctc
    43861 tattaagtct atttgtagta acaattccag cagtgttggt agcttttgta ttaaaaggga
    43921 tgtcaattgg ttatacaata aatagttgta tattggcatt aaaatttaaa agtataaaaa
    43981 tgattttaat aatattattt aaaaatttga ttattattcc atgtgcaatc atattagcat
    44041 taattagttt aagttatttt aaagaaatgg tttatgaatt taaaaagaaa aatagaaaaa
    44101 atatgcaatt tttaattaag aggtatatac taaatattat aattataata gcactatcat
    44161 tggggttgca attaatatta aatactgtaa gtattggtat tattaagttc ctagctaaat
    44221 aaaggagttt ataatggata ttatagaggg atatatagat tacataaaag ataaaaaaaa
    44281 attatcagag aacacagtgg catcatactt tatggatata aaaaaatata tggaatatat
    44341 aaatcaaaaa agcattaaac tagtagatat agtagaaaat gatattataa gttacttaat
    44401 aaagttggaa aaagataatg tatcaatagc tacaatagcg aggatgatat cctctataaa
    44461 atcttttcat gattatttat ttttaaatca tatatgtaca aataatcctg caaaggatat
    44521 aaaaaaacct aaaataaaaa aagaaaatat aaatatactt acagaagaag aaattgaaag
    44581 actattaaat tttccaaagt taacaacacc taaattgatt agggacaagg ctatttttga
    44641 agtattatat ggtactggta taaaagtatc tgaattagta gagatgaata tagaggatat
    44701 tgacctagat attgattata tatattgtaa ttcatgttgt aaaaatcaaa gagtgatacc
    44761 actttgtgat attacaaaac tttacttaga aaagtatcta aaagaagcaa gacctaaaat
    44821 ggctgtagaa ggtgaaaaat cattattcgt aagttcatta ggtcaaaggt ttacacgaca
    44881 aggtctttgg aaagtcataa aaaaatattc taatctagcc aatatagata aaaatataaa
    44941 tcctacaatg cttagacatt catttgctat tcatttgtta aatgaaggtg ctaacattgc
    45001 tgttgtaagt aaaattttag gaaatgttaa tttatctagt ttgcaagtat atttaaatca
    45061 tatagataaa aacgtgagaa gagaaataaa ggaaaaacat cctagaaatg atgttgatgt
    45121 agaattacaa aaagcagaag ctaaatagac tatatagcaa attttataat ttaaaaggag
    45181 tatatttata tgagcagagt aatttggatt gtaattgata gtgttgggat aggagcatta
    45241 cctgatgctg aaaaatttgg tgatagtaag gatgtaagta cccttggaaa tatatttaag
    45301 gaatatccag atattcagat tcctaatatg agaaatttag gtataggaaa catagatggt
    45361 atagattttt ttgaaagtat aaaagaacca ataggttgtt ttggaaagtg taaggaaatg
    45421 tctcagggaa aagatactac tacaggtcat tgggaaatga ctggtataat agttgacaaa
    45481 ccatttaaga catttgaaca tggattctct aaggagataa tagaagagtt tgagaaaaaa
    45541 actggaagaa aagtagtagg aaataaacca gcttcaggaa ctgtaataat agatgagtat
    45601 ggagagcatc aaataaaaac tggagatgta atagtatata catcagctga tagtgtattt
    45661 cagattgctg ctaatgaaga agtaattccc ctagaagaac tctataatat gtgcaaaatc
    45721 gctagggaaa taatgatggg ggataatgca gtagctagag taattgctag accattcata
    45781 ggtaagaaaa aaggtgagtt tgtgaggact tcaaatagaa gagattattc actagaccca
    45841 tttgaaccaa cagttcttga taatataaaa gaatcaggac ttgatgtttt agcagttgga
    45901 aaaatagaag atatttttaa tggaaaagga attacagatg ctatccacac taaaagcaat
    45961 atggatggag tggatgaaac tttaaattat atgaaacaag ataataaagg tctaatatat
    46021 tcaaatctcg ttgattttga ttctaaatat ggtcatagaa gagacccaga aggatataag
    46081 aaagctcttg aagagtttga tagtagactt cctgaaataa tggctaatat gagagaagat
    46141 gatattttaa taatcaatgc agaccatgga aatgacccaa catataaagg tacagaccat
    46201 acaagagaat atatacctgt tatgatttat ggcaataaga ttaaaaaagg ttttaattta
    46261 ggagtaaaag atacttttgc tgatattgga gcaactgttg cagatatact aaatgtaaag
    46321 ttgcctaaac atgggtcaag ctttaaagaa gatttatttt aaactataaa atgataaaaa
    46381 cttagcttaa taggatggag agattggaat gtttgaaaag atagcacaaa gtagtaaatt
    46441 tataaattct aaaagtaata taaaacctaa gattggtttg atattaggtt ctggactagg
    46501 tgatttagca aatgatatag aagaccctgt cacaattaaa tatagtgaaa ttccaaactt
    46561 ccctgtatca acagtagcag gacatgctgg acaacttgta attggaaaac ttgaaggaaa
    46621 agaagttatt gctatgcaag gtagatttca ttactatgaa ggatattctc agaaagaagc
    46681 tacttttcca gtaagggtta tgaaagaatt aggagtcgag attcttatag taactaacgc
    46741 tgctggaggc gtaaataaag aatttaaagc aggagatttg atgcttataa gagaccatat
    46801 aaactttagt ggaagtaatc cattagtagg aaaaaatgat gacagatttg gtgctagatt
    46861 tccagatatg tcagatgcat attcttctaa atatgtagat gtagttaaaa actgcgctaa
    46921 agaatgtaat atagatgtca aagaaggcgt ttatatgttt tttagtggtc caaactatga
    46981 aacaccagca gaagtgagaa tggcacaaat tttgggagct gatgctgtag gtatgtctac
    47041 tgttccagaa gtcatagtag cttctcactc caatatagga gttattggta tatcttgtat
    47101 aacaaatatg gctgctggca tcttaaatca accactaagt catgaggaag taatagaaac
    47161 tactcaaaaa gttaaatcag aatttatgaa tttagttaaa acaacggtta aaaatttata
    47221 agttaaagct tattatatat tttttgagga agtttataat aaaatagtta atgtttaatt
    47281 tgaaaagaat taataaaaag ctgtcttgat atagaaagtt atttctaata aaggacagtt
    47341 tttttataat actacaacat ttgaaagtgt tttaaagttt aatatttatt taaattatta
    47401 tagattagaa tggagatttc aattatgaga atgtatgata ttataaaaaa aaagagagat
    47461 aacttagagc ttagtaaaaa agagataaac tactttatag aaaactattc aaagggagat
    47521 atacctgact atcaagcttc tgcactatta atgtcgattt atttaaataa gatgaataaa
    47581 caggaaactg tacatcttac agaagcaatg atgaactctg gagacatgat aaacctgtct
    47641 gaggtagatg gaataaaggt agataagcat agtactggtg gtgttggaga taagactacc
    47701 atagctctta tacctttggt tgcttcatgt ggagcaccag ttgcaaagat gtcaggtaga
    47761 ggtcttggtc atactggtgg tactattgat aaattggagt ctataccagg tttttcgaca
    47821 gaaatggaga taagtaaatt tattgattct gtaaataagt ctaaaatagc agtttgtggt
    47881 caaagtgcaa aggtagcaat tgcagataag aaaatatatg ctcttagaga tgttacagca
    47941 actgttgata atatatcatt gatatcatca agtgtaatgt ctaaaaagtt agcttcagga
    48001 gctgatgcaa ttgtgttaga tgtaaaaact ggtgatggag catttatgaa gacattagaa
    48061 gagtcttttg agttagctaa atctatggtt gatataggta ctggtatgaa tagagataca
    48121 atagggattg taacagatat ggatgaacct ttaggttttg cagtgggaaa ctctttagaa
    48181 gttatagaag caattgagac attaaaagga aatggcccta aagaccttgt tgagctatgt
    48241 gaagtattag gagcatatat gttggtttta tcaaaggtag cacatgattt tgaagatggt
    48301 gttgaaaaga taagagaatc aataaaatct ggtactgcta tacaaaaact caaagagttt
    48361 atagagaatc aaggtggtaa taaagatgta gtagatgatt attctttatt caaaaaatca
    48421 aagtataaat atccagttaa atcaattaaa tcaggttata taagtaaaat taaagctgaa
    48481 gatgttggtg tatcagctat gattcttgga gcaggtagag aaactaaaga tgatatttta
    48541 gatttatcag cagggataat tttagaaaag aaagtaggag attatgtaaa tgaaggtgaa
    48601 gttttagctt acatgtatta taatgatgaa caaaagcttt cattggcaaa gggaagattt
    48661 attgattcct actctattgt agattcaaaa atagaaaaaa ataaattaat atatggtatt
    48721 gtaactaaag aagagattaa aaagttttag aaaattttag aaaagtgatt ataaaaggcc
    48781 taacatgtca aaaataatgt taggtttttt attttttgat aaactataaa ttataccaaa
    48841 aatatatatt ttaaaacatt ttttacaata taaataagtt tattctacaa gatgattgaa
    48901 aattaaagta tctagtttta taatagaaaa tggataaata tacataagga gtagattata
    48961 ttatgaactt taaagaaaat agattgaaaa tagcagtatt aataattgtt attttgatta
    49021 tcttagcagg tatatttgtg tttatacaaa taggtcctta tgataaaaat aacaaaaaag
    49081 atgtaattat tgacgttcca agtggagcat ctgttggaaa aatatctgac atattatatg
    49141 aaaataaatt aataaagaat gaactattat ttaaactttt ggtaaaggtt agcaataaag
    49201 ctccgtctat aaaatcagga acatacttac ttaatcaatc gtattcaaac aatgatataa
    49261 taagtctttt agtttcaggt aaaatatatc aggatggtat aaaagtgact attccagagg
    49321 gagctacatc taaagaaatt atagcaatgt tagtaagtaa aaatttagga gataaagcta
    49381 cttttgaaaa tttaataaaa aaacctcaag aattttatga taaatttcca tatttgaagg
    49441 aagatggaat aacctcttta gaagggttct tgtatccaga aacttattat tttaattcaa
    49501 agaaacaatc agaagaagat attttaagcg aaatgttaaa agtatttgac agtaaataca
    49561 cggacaagtt taaaaagaaa caaaaagaat taaatatgac tcttcaagaa gtaatggaaa
    49621 tggcatcaat aattgagaaa gaagctgttc ttgacaaaga cagacctata atagcaagtg
    49681 tgttttataa tagacttaag gtaggtatgc ctcttcaatc agatgctaca atacaatata
    49741 tatttgaaga gagaaagaaa attgtaacat atgatgattt aaaaattgat tcaccatata
    49801 atagttataa aaataaggga ttacctccta caccaatatc aaatcctggt ataaaatcta
    49861 ttgaagcagc attatatcca gagaaaacag actatttata ttttgtagct aagattgatg
    49921 gtggaaataa ttatagtaca aactatcaag accatttaaa atacgttaaa gaatataaag
    49981 aagcaaggga taaacaatca aaagatacaa aagcaacaaa taaagaaaat acaaaaaaat
    50041 aaaccatgat tataaaaagt ctaacagggt aagaataatg ttgggctttt ttattgaaaa
    50101 ttgttattct tagatttata ataaagaaag tgtaaatata tataacaata tgaatttaaa
    50161 aaataataaa ttaatataaa acttgaatat ttattatttt agggagtgtt tttatgagta
    50221 atatagttaa tgatttggtt gaagattata taagagtgac attaaaagaa aaagaaggat
    50281 ttttaaaaga tttagaaatt tatgcagagg aaaatagtgt tcctataata cataaagaag
    50341 tttcagattt attaaaagtc ttgttaaagg ttcaaaaacc aaagagaatt ttagaagtag
    50401 gatgtgcaat tggttattct tctatttttt ttgcaagtat tataggaaaa gatgcagata
    50461 taataactgt tgagagaaat gaaaaaatga tagaaaaagc aaaagaaaac ataaaattag
    50521 ctggatttga caataacata actatactag aaggagatgc agaagaaaaa cttagtcaag
    50581 tccaaggtga atttgacctt atatttatag atgcagccaa aggtcaatat aagctatttt
    50641 ttgatttagt tatagataaa ttaaaagatg ggggactttt agtatctgat aatattttgt
    50701 ataaaggtat ggttgctcat gatgattttg ttgtaagaag aaaaaagact atagttaaaa
    50761 gaatgagaaa ttatttagat tatatatgta attgtgacta tttaagcaca tcattggtac
    50821 caataggtga tggtgtagca ctaagttata aagaaagtaa aagaggtgat gtagatggaa
    50881 ttaaataaag tagaactttt agctccagca ggtgatttgg agagattgaa aatagctata
    50941 acatatggag cagatgccgt ttatataggt ggagaaattt ttggaatgag gtctgcagcg
    51001 aagaatttta gtaaggaaga tatggcagag ggagtagctt ttgcacatga aagaggcaag
    51061 aaagtattcg taacagtaaa tataatacct cataatgaag attttctaca attagaagac
    51121 tatttgttgg aactagaaga gataggaata gatgcagtta taatagctga cccaggagtg
    51181 cttagtgtca ttaaaaaagt aattccgaat atggaaatac atttaagtac acaagcaaat
    51241 acaaccaatt atctaagtgc aaacttctgg tacgagcatg gaataaaaag agttgtagtg
    51301 gcaagagaat tatcttttga tgaaatatct gagataagag caaaaacacc tttagatatg
    51361 gatatagaag cgtttatgca tggtgctatg tgtatttctt attctggtag atgtttaata
    51421 agcaactata tgactggaag agatgcaaac aaaggttctt gtgctcaatc ttgtagatgg
    51481 caatatcatt tggtggaaga aaaaagacct ggtgagtatt ttccaatata cgaagatgaa
    51541 aggggtacat tcttctttaa ctcaaaagat ttatgtatga tagagtatat accagagtta
    51601 atcaaatctg gtataacaag tttaaaaata gaaggtagaa tgaagactgc atactatgta
    51661 gctactgttg taagagcata cagaatggca atagatgaat tttacagagc cccagaaaac
    51721 tggaagttca atccaatgtg gatggaagaa ctaaaaaaag gtagtcatag acattttaca
    51781 tcaggttttt acttgaataa accaacaaca gaagaccaaa attatcaatc agcatcatat
    51841 gttagaaact atgattttat aggtatagtt agagaaacag aagatgaaga tggtcttatt
    51901 gttgttgagc aaagaaataa aatgtgtgtt ggtgatgaaa tagaagttat gggaccatat
    51961 aaagaaacta tgtttactaa aattgaagaa atgtacaatg aagaaggaga agctatagaa
    52021 tcagctcctc atcctagaca aatagtgaag ttaaagctat cagtaaaagt tggtaaagat
    52081 tatatgctta gaaaagttat agaagaaaag gttgaagaat aataaaaaat tgataaaaat
    52141 agtattagcc ccttagaaat tgtcaataat gaaattatct aaggggtgat ttcatgtcaa
    52201 aaaagaagac tccatttttg aaaaaagtgg gaaagagaag ctggtgtata tttactataa
    52261 ttttaataat ttatagtgtt ttaatttata ggctagttga tatacaagta ttaaaaggtg
    52321 ataaatacaa acaaagtgtt gaatcacaaa gcgttgagaa agtcgagtta aatagtggaa
    52381 gaggaataat ctatgataga aacaacaaga agcttacaga tacaagtaaa tctcaggtat
    52441 taattgttga aaaagaaaaa ttaaataata actataaaat tttggaactt attaagaaag
    52501 ctactaagat gaatgattta gatatatata aagctgtaca agagcaattg acacgcccta
    52561 taatacagat tcagactaaa aatatcgata agagtatgaa aaaagaactt gagaaaaatg
    52621 gaataatggt agaagaaaaa actatgaggt acgcaaaaga tggtttatta tcacatacaa
    52681 taggatatat aaaagaagat gataaatcag gtcaatcagg aattgaaaag agtatggata
    52741 gtgtacttag aaattcaaat gaaaagtata taagtgcttt taaagctgga gatgcaggaa
    52801 atgaaaaatc tctaaacata ctaaagggaa gtgtaaaaac tgttgataat aaggataaag
    52861 atagacatct aaaaactact attgattata acattcaaaa aaaattagaa caaatactaa
    52921 ataaagaaga aaatcctaca gcagcaataa tctcggaagc atctacaggt gaaattcttg
    52981 caatgtgctc tagacctaat tttgaccaaa atgacatatc aaaaagttta aaaggaaaaa
    53041 atggggaatt tgaaaataga gttataaaag cgacatatcc accagggtct gtatttaaga
    53101 tggtagtttt attttctgca cttgaaaatg gtgttataga tgagaactat acttacaact
    53161 gtacaggtaa gactaaagtt gggaatacta atgaaatact gcgatgtaat aaaagagatg
    53221 gtcatggttt ccaaaactta agacaagctt tttctaattc atgcaatcct gcatttttag
    53281 atatagctat gaaacttgga aaagaaaaga tactcaaatc tgctgaaaaa ttacatttat
    53341 ttgaaaaggt tgatattggt cttgatgaag aaaaaattag agaagctcca aaaaacatat
    53401 caataagaaa tttagctata ggtcaagaaa atatagaatt tacaccactt caaataaatc
    53461 aaatgactca aataatagct aataatggaa cattcaaacc actatactta tataaaagtc
    53521 ttgtggacaa taatatgaat acaataaaaa cttataagtc ttcaaaaaaa gaagaactta
    53581 tttctcctta tgtgtgtaca caagttaaag aatatatgaa gtctgtatct aggattggta
    53641 cagctaaaga cctaaaagat attgaaggtg gttgtggagt taaaactggt acagctcaaa
    53701 gtagtttaaa taaaaaagct atagaccacg gatggataac aggattttat cctgaagaaa
    53761 gacctaagta tgtaataact gtgttagtag aaggaactca aaaaggaaat aaatctgcaa
    53821 cacctatatt taaggaaata tgtgaaagta taaaataaat tatgtactat aaaatttaag
    53881 gatatatcgt aactttgatt ttagatagaa aactttgtct aatgatgtac gatatatcct
    53941 tttaaaatat agtattaact ttcttgaaca agctcttttt ctagtttttt aagagctttt
    54001 ttttcgattc tagatacata tgaccttgat atatcgagtt tttgtgcaat ttctctttgt
    54061 gtcttatagc cataaggagt taacccatat cttaactgga caatttctct ttctctagga
    54121 gtaagaattt tgtttagttg ttcgtaaagt tttccaactt gaacttttag ttcaacttca
    54181 tcaagaacat agttagcctc tgtgccaagt atatctaaaa gacttatttc attacacact
    54241 actctttata tgttattggt attactggtc ttcatgtaat ttcaaatgaa ccattaattc
    54301 aaaagaatct agtttttgtt ctttagactt ataataaatt gctttatcaa ttacagattt
    54361 taggagttta ttttttccaa gggaatctga atcatgataa agtgataaca cagttttaat
    54421 atcttctatt attttagaat tgtctttttt aacagtactc aatctatttt ttactgtctt
    54481 aattgaatct ttaatttctt cagttcttga acttatattg ttggaccttt caataaatgt
    54541 atcttcatca tatattccac gttctaataa atcatataac ttattttttt gtttacctaa
    54601 ttcttttaat tcagtttcta agccctttaa gactcgtttt tgaaattcta tattactatc
    54661 tttattattt ttatctaagt ctaaatcaca tgcttttaat tgatattttt ttaccgtgtc
    54721 ttccaaagat tttaaaatgg cagcttcaat aaattcaaat cgagaacttt tattgcaacc
    54781 aggatggtaa caaattatat agtcgtggtc tgcatatggt ctatatacta atggtctacc
    54841 acaatttttg caaatcacta ttcctgcaag tggatttttt atttgattac catagctgac
    54901 atgaccgttt tttttaagta tattttgagc ttttttccat gttatttcac ttattaaagg
    54961 ttcatgcttt ccttttgctt caatccattc atctttaggt cttagcttaa cagtttttat
    55021 tttatttgga tttttagatt ttttatagtc ctttttttgc cactgaatgt agccacaata
    55081 taccttattt tttattatat ttgtaattgc agaattgtac caattagcac ctgtagcagt
    55141 tttaatgcct aagttattta ggtattttga tattttacta caacccatat cttcattaat
    55201 atataagtca aagatagtcc taactacatc agcatcctta tttggtacaa gtattctttc
    55261 ttttctacct gttgttacag catcatatcc aaatggagca gaagttccta tgaaattccc
    55321 ttcttcaact gattttattc tacctctttg cattcttctt gatataagtt ttaattcttt
    55381 tcttgccata aatgcttcaa attctgaata ctcctcatca aattcatctg ttaggtcgta
    55441 tgtttttcta ggtgtgatga ttttggtttt actttctttg aatgtttcta atatgatacc
    55501 ttggtctttc atgtctccac gaccaagtct gtctaagtcc attactaaga ctgcatcata
    55561 tttattttct tcaacttctt ttagaagttc aagcattttg ggtctatgta ttatactttc
    55621 accagaaact aattcttctt ttatctcaat tacatctaag ttttgttctt ttgctagttt
    55681 tagtagggtt gatttatgtc tacttaaagt ttcaaattcg ccttgttttt ctgcctcttc
    55741 atcagcacgg gattttctta agtatatggc aacactcata ataagtaatc acactcctta
    55801 tgcactatta tatagtaaat caatagagtt taaaatatag aaaataagca tgtcaaaagt
    55861 taatttaact ttcaacatac ttattttcta taatattaat gccttataat cctttaatat
    55921 catttagatt agtttttgca tattcattat ccgggttaag ttcaagcgcc ttattgtaag
    55981 attctctagc tttattaaat tctgatttac tcataaaaat aacacctctt aaattgtaag
    56041 catcactagc gtctatttta taagcttcat tatttttatc tagatattct aaggatttat
    56101 caattgactt taaagcctca tcatatcgtt gtaactctat ataaatatat gattgtgtat
    56161 aatatgcaag tggattttta tcatcaattt ctatagcttt tttgatatca atagcagctt
    56221 tattataatc cttatcttca tctttttttg tttctgacct agcgattttg acataattag
    56281 agctaagagc tcttttaaag tctttatgcc ataataattt atattcatta tcttcgatta
    56341 taaaatatct atcaaaattt gcagtatctt ctttattttc agtgatatac tttatagtat
    56401 cagtttcttt tattttaaca agatttgtgt actctttatt agaatatttg tatttctcta
    56461 ctttattttc ttcaccaagt ttataatctt ttagggtttg aagtttaaat aaagtatctt
    56521 gatattcttc aaatttttct agactgacac tttccttact gtctttactt aataaattat
    56581 atgctacttc gtagtctttg atttctatac tatcaaggta ctttttagct acttctgtag
    56641 cactttcttt tggagcacat ccaaccattg atatcataat taaaaatgat actaatattg
    56701 ctgttatttt tttactgatt atcataatat aatccccctt atattgtctc tcactaattt
    56761 tattttatca ccatattcta ccaaagtcat taatttaaga atgatatttt tcattaatat
    56821 tatattaagt aataataatt tttaaaattt atggtaacat tttcctgaaa agtattatat
    56881 aatttaggtg aaaatattta agagaaggag atattatgaa aattaaaaaa ttattaacaa
    56941 tagggttatc tttatctata tttattgcta gttgccctat atctgcaaat gctcttgata
    57001 aaattgaatc aattaaaggt gcagataaat acgaaactgc tggtataatt gcagacaaac
    57061 aaaactatac tacagctata ttaattaatg cagacagtac aatggctgat ggattaagtg
    57121 caagtggact tgcaggagct ataaacgctc caattctttt gactaagaaa aataacatac
    57181 caaatgctac acttaaaaga ttagaaaagg caaaaaaagt atatataatt ggaggagaaa
    57241 actctattaa taaatatact gaaactgttc taaaaggcaa aggaatagag attaaaagac
    57301 ttcaaggtag tgatagaatt aagacaagct ataatgttgc aaaagaaata aattcaatta
    57361 acaaagtaaa taaagtcata ttgactaatg cttttaaagg agagcctgat gcaatgagtg
    57421 ctgcacctgt agcagttaga gataaagcag ctatagtatt aactgatgga aaaagtgttc
    57481 catttaatac aactggtgta gaaagttatg taataggtgg tacttcatca atgaatgaca
    57541 aactagttaa ggatactaat tcaactagat taggtgggac agatagatat gatactaaca
    57601 aaaagataat aaataaattt tatagtggag taaaagaatt ttatatagca agtggtactg
    57661 atttagtgta tgctcttgtt ggttctacca ttgctaaaaa taatgctatt gtgttagttg
    57721 gtaatgatag taataaaagt gtattgaaaa gtacaattaa attaactgct ataggaaatt
    57781 tgagtgatag tatattacag cagtgtttaa atgtaacaaa aaatataggc gattctaata
    57841 ctggaagaga atatacagta gaagatgctg ctaatgcagt gaaaaaacta ttaggtgtga
    57901 agaaatttta ttacgaacct aattattcag aagatgaaga tttaatattt ctactagata
    57961 aaaattttga tttttcagga aaagagtact atgtatttgc ttacattaaa cagggtgcag
    58021 agggtgatgt tagatactgt gtagctaaaa atgatatgag caaagtttat acatatgatg
    58081 ttaatggtaa tatgaaagta tgtaatccag atgattataa aaataaagga agtataacag
    58141 aggaacaagc aaaacagata gcattaaaag aatgtgctaa gagacataaa gtcagtatca
    58201 ataacttagt agttgatatg atagatttta gagatgatat atatagtggt aatgtctatg
    58261 ctgttaaaat caattcaaaa aatagtaatg gtacttttgg ttggtttttt atagatatag
    58321 aaactgggaa aatttataat taaaattaga acttttaaac aataattata taatttacca
    58381 taatatgtta taatattttt agcaagaaga tttaacttac aatctataga gtggagttca
    58441 tgtacaaaag attatcctcc caacgattcg aagggaggtg agttcatgga taactttttg
    58501 tttaatgttt tagctagttt aacagctagt gtggtagttt acttaatcag taaactattc
    58561 aaaaaagcaa aaagccactc tcgc
//
LOCUS       DAMJVX010000008        57062 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMJVX010000008 DAMJVX010000000
VERSION     DAMJVX010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 57062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57062
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.008"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(<1..153)
                     /locus_tag="PC407_000422"
     CDS             complement(<1..153)
                     /locus_tag="PC407_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2495904.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARY"
     gene            complement(327..1193)
                     /locus_tag="PC407_000423"
     CDS             complement(327..1193)
                     /locus_tag="PC407_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2495905.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNVEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTREYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(1419..2288)
                     /locus_tag="PC407_000424"
     CDS             complement(1419..2288)
                     /locus_tag="PC407_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2495906.1"
                     /translation="MDYKTIILNRKSVREYKNIDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNKSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(2794..3015)
                     /locus_tag="PC407_000425"
     CDS             complement(2794..3015)
                     /locus_tag="PC407_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495907.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(3516..4496)
                     /locus_tag="PC407_000426"
     CDS             complement(3516..4496)
                     /locus_tag="PC407_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495908.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRVYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(4660..5532)
                     /locus_tag="PC407_000427"
     CDS             complement(4660..5532)
                     /locus_tag="PC407_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2495909.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(5696..6247)
                     /locus_tag="PC407_000428"
     CDS             complement(5696..6247)
                     /locus_tag="PC407_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2495910.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSYVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(6267..7169)
                     /locus_tag="PC407_000429"
     CDS             complement(6267..7169)
                     /locus_tag="PC407_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2495911.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRTQINNKCDLCGECIKVCLKDAICIMT
                     EKMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(7184..9556)
                     /locus_tag="PC407_000430"
     CDS             complement(7184..9556)
                     /locus_tag="PC407_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HDF2495912.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAEGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(9775..10464)
                     /locus_tag="PC407_000431"
     CDS             complement(9775..10464)
                     /locus_tag="PC407_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495913.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMAGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEEFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(10705..12084)
                     /locus_tag="PC407_000432"
     CDS             complement(10705..12084)
                     /locus_tag="PC407_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2495914.1"
                     /translation="MLNFMNELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGSYFSNINYLFYILAFIAWGGYYNYFYKMDYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPHLESIKIQIYSNLIIILIYLVFSFFRKS
                     IKLISQDKRNYVYLLFALLANTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEICLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENINKYIRIKGTITKSFFVLKCEN
                     SKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPL
                     EQDTDSWGD"
     gene            complement(12078..12791)
                     /gene="rgbR"
                     /locus_tag="PC407_000433"
     CDS             complement(12078..12791)
                     /gene="rgbR"
                     /locus_tag="PC407_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HDF2495915.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDDEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNAAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            complement(13042..13602)
                     /locus_tag="PC407_000434"
     CDS             complement(13042..13602)
                     /locus_tag="PC407_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HDF2495916.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            complement(13698..14501)
                     /gene="zupT"
                     /locus_tag="PC407_000435"
     CDS             complement(13698..14501)
                     /gene="zupT"
                     /locus_tag="PC407_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDF2495917.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            complement(14652..15815)
                     /locus_tag="PC407_000436"
     CDS             complement(14652..15815)
                     /locus_tag="PC407_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2495918.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IEIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            complement(15875..17062)
                     /locus_tag="PC407_000437"
     CDS             complement(15875..17062)
                     /locus_tag="PC407_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495919.1"
                     /translation="MEAITAFCLVLISLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTIVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            complement(17425..19125)
                     /locus_tag="PC407_000438"
     CDS             complement(17425..19125)
                     /locus_tag="PC407_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2495920.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            complement(19381..19656)
                     /locus_tag="PC407_000439"
     CDS             complement(19381..19656)
                     /locus_tag="PC407_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2495921.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIHAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            complement(19775..20326)
                     /gene="mobB"
                     /locus_tag="PC407_000440"
     CDS             complement(19775..20326)
                     /gene="mobB"
                     /locus_tag="PC407_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDF2495922.1"
                     /translation="MKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGYRVGVIKH
                     TCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQLYEDMDLI
                     IIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLNDYKNISKY
                     IESCIKNEILSISNVEIKNILKE"
     gene            complement(20331..21512)
                     /locus_tag="PC407_000441"
     CDS             complement(20331..21512)
                     /locus_tag="PC407_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDF2495923.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGEVFNKVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMIILNVKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGILGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGIHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVEIVI
                     R"
     gene            complement(21723..22457)
                     /locus_tag="PC407_000442"
     CDS             complement(21723..22457)
                     /locus_tag="PC407_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495924.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSVVIEKSDHEKSAVSLENFR
                     RIDLGEYVGVEESRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            22819..23724
                     /locus_tag="PC407_000443"
     CDS             22819..23724
                     /locus_tag="PC407_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2495925.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKGVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            complement(23884..24726)
                     /locus_tag="PC407_000444"
     CDS             complement(23884..24726)
                     /locus_tag="PC407_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2495926.1"
                     /translation="MTTSSKKLDTISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSSSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGIV"
     gene            complement(24710..25468)
                     /locus_tag="PC407_000445"
     CDS             complement(24710..25468)
                     /locus_tag="PC407_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2495927.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            complement(25485..25955)
                     /locus_tag="PC407_000446"
     CDS             complement(25485..25955)
                     /locus_tag="PC407_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2495928.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDELLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            complement(25955..26698)
                     /locus_tag="PC407_000447"
     CDS             complement(25955..26698)
                     /locus_tag="PC407_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HDF2495929.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLELGDMAIIGSYF
                     KDNYKDFGNVSVEHVKTFMDEIKKIREEL"
     gene            complement(26702..27115)
                     /locus_tag="PC407_000448"
     CDS             complement(26702..27115)
                     /locus_tag="PC407_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDF2495930.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDIECEYIILSDLYGASVCTAMLRLTNLHNVKLFSGMNLNMVLELLTR
                     FSDELTVEDIEQLVEESRRGIQSVTLQIKEEEEVF"
     gene            complement(27141..27893)
                     /locus_tag="PC407_000449"
     CDS             complement(27141..27893)
                     /locus_tag="PC407_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2495931.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDYLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            complement(28133..29527)
                     /locus_tag="PC407_000450"
     CDS             complement(28133..29527)
                     /locus_tag="PC407_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2495932.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTALPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGFDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            complement(29547..29756)
                     /locus_tag="PC407_000451"
     CDS             complement(29547..29756)
                     /locus_tag="PC407_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDF2495933.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            complement(29785..31230)
                     /locus_tag="PC407_000452"
     CDS             complement(29785..31230)
                     /locus_tag="PC407_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2495934.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSSNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTNKYKLSKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISVISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            31340..32134
                     /locus_tag="PC407_000453"
     CDS             31340..32134
                     /locus_tag="PC407_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2495935.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQYEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            32185..33507
                     /locus_tag="PC407_000454"
     CDS             32185..33507
                     /locus_tag="PC407_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2495936.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(33608..34825)
                     /gene="cdeC"
                     /locus_tag="PC407_000455"
     CDS             complement(33608..34825)
                     /gene="cdeC"
                     /locus_tag="PC407_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HDF2495937.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDECNPCNPCNPCKPNPCNPCKPNPCDDKCECHDKCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSIPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(35008..35188)
                     /gene="ssrS"
                     /locus_tag="PC407_000456"
     ncRNA           complement(35008..35188)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PC407_000456"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(35303..36208)
                     /locus_tag="PC407_000457"
     CDS             complement(35303..36208)
                     /locus_tag="PC407_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495938.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            36420..36818
                     /locus_tag="PC407_000458"
     CDS             36420..36818
                     /locus_tag="PC407_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495939.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGGQQKKSKGLFKKTKKSNPHE"
     gene            complement(36941..37957)
                     /gene="ccpA"
                     /locus_tag="PC407_000459"
     CDS             complement(36941..37957)
                     /gene="ccpA"
                     /locus_tag="PC407_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HDF2495940.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(38210..38410)
                     /locus_tag="PC407_000460"
     CDS             complement(38210..38410)
                     /locus_tag="PC407_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495941.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            complement(38444..38623)
                     /locus_tag="PC407_000461"
     CDS             complement(38444..38623)
                     /locus_tag="PC407_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495942.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(38805..39011)
                     /locus_tag="PC407_000462"
     CDS             complement(38805..39011)
                     /locus_tag="PC407_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495943.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(39059..39484)
                     /locus_tag="PC407_000463"
     CDS             complement(39059..39484)
                     /locus_tag="PC407_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495944.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            39656..39883
                     /gene="acpP"
                     /locus_tag="PC407_000464"
     CDS             39656..39883
                     /gene="acpP"
                     /locus_tag="PC407_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2495945.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            complement(40027..41031)
                     /locus_tag="PC407_000465"
     CDS             complement(40027..41031)
                     /locus_tag="PC407_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF2495946.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(41045..41947)
                     /locus_tag="PC407_000466"
     CDS             complement(41045..41947)
                     /locus_tag="PC407_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2495947.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKKEK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(42142..43317)
                     /locus_tag="PC407_000467"
     CDS             complement(42142..43317)
                     /locus_tag="PC407_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2495948.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(43404..44249)
                     /locus_tag="PC407_000468"
     CDS             complement(43404..44249)
                     /locus_tag="PC407_000468"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HDF2495949.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(44347..45144)
                     /locus_tag="PC407_000469"
     CDS             complement(44347..45144)
                     /locus_tag="PC407_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2495950.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(45163..46173)
                     /locus_tag="PC407_000470"
     CDS             complement(45163..46173)
                     /locus_tag="PC407_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2495951.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(46192..46974)
                     /locus_tag="PC407_000471"
     CDS             complement(46192..46974)
                     /locus_tag="PC407_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2495952.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(47033..48169)
                     /locus_tag="PC407_000472"
     CDS             complement(47033..48169)
                     /locus_tag="PC407_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDF2495953.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLKPLASGDKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(48877..49344)
                     /locus_tag="PC407_000473"
     CDS             complement(48877..49344)
                     /locus_tag="PC407_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HDF2495954.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKSEKWVDVCKNW"
     gene            complement(49423..49965)
                     /locus_tag="PC407_000474"
     CDS             complement(49423..49965)
                     /locus_tag="PC407_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495955.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDIGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITESTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(50017..50703)
                     /locus_tag="PC407_000475"
     CDS             complement(50017..50703)
                     /locus_tag="PC407_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2495956.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            complement(50691..51563)
                     /locus_tag="PC407_000476"
     CDS             complement(50691..51563)
                     /locus_tag="PC407_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2495957.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDREKFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVRGCSI"
     gene            51698..52498
                     /locus_tag="PC407_000477"
     CDS             51698..52498
                     /locus_tag="PC407_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HDF2495958.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            52523..53092
                     /gene="yfcE"
                     /locus_tag="PC407_000478"
     CDS             52523..53092
                     /gene="yfcE"
                     /locus_tag="PC407_000478"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDF2495959.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(53345..54397)
                     /locus_tag="PC407_000479"
     CDS             complement(53345..54397)
                     /locus_tag="PC407_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HDF2495960.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(54604..55830)
                     /gene="pepT"
                     /locus_tag="PC407_000480"
     CDS             complement(54604..55830)
                     /gene="pepT"
                     /locus_tag="PC407_000480"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDF2495961.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCREKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            complement(55921..56972)
                     /gene="ytvI"
                     /locus_tag="PC407_000481"
                     /pseudo
     CDS             complement(55921..56972)
                     /gene="ytvI"
                     /locus_tag="PC407_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
BASE COUNT        18533 a        10116 c         5972 g        22441 t
ORIGIN      
        1 gtatctagct atcttaaagg ctaagatgat agaaggatta tacctaccat tttctaatga
       61 acctatagtt tgtcttgata cttgcaaaat agaagcaagt tcttcttgtt ttatgcctct
      121 ttcttttctt atttcttcta atctattttt cacatctatc cccctttaat atctcaacta
      181 cggaaagtct actttccata attatacaac tatatttcat ttatgtaaag cttgctttcc
      241 atatttacaa aaaaataaca gactattttc agcctgtaat tttaaaattt catgttgttt
      301 attaaattta atgttattca ttaactttaa cttggtttct gaattggaat ccaaacttct
      361 gtagtataat caggagcatt gatatcgcca ttgggataaa gttcaatatc tggtaagtta
      421 gcatacacat acccagaact tggtaaccac tcagtaataa ttcttttttg caattcctgt
      481 attgccagag catttggcat tggagaaaca cattcaaata ctgcccatgt acctgatggt
      541 attaagtatt cacgagtatc ttctaataca ggcttatctg ttggtgcagc aatataataa
      601 tctaaaccct tactatttgt acacacacta actccaagca atccacaagg ttcgttattt
      661 attaactctg aaagtttatc aattgttcca ttttttatgc atttagccca taacttagga
      721 actttctcaa agcactcttc tacaatcatg ttcatacttt cttttatacc aacaatacga
      781 aaccctttct tttctacaat acgatattgc atttctacat ttccttttac ataaatgctt
      841 aatgtcatct taggatatgc ttttaaaaca acaccctttg ctctagctgc agatggagaa
      901 atgttatgta tattttgaaa agctctatta aaagatgttg gtgaatcgta accatacttc
      961 tcagatagtt ctaaaatttt gatgttactg ttctgaagtt caaatgcagc cttagtcata
     1021 cgtcttcgtc taatatattc agataatggt acttctgcaa tatatgaaaa cattctttga
     1081 aaatgaaagg ttgaacaaca tgcaattttt gctgcttctg cataatccac tccactttcg
     1141 agattttctt caatataagt aatagcttga tttaatttat ttatccattc catttgatat
     1201 tacctccaag caaattatac agtttaataa aataatatac ctctctattc atgcacaaaa
     1261 ttgtaagcat ttaaattcat aagtattttt caatcaatac aataaatata aaaattgtat
     1321 tatacaataa aaaagttcaa taacatttta ttataaaata ttattgaact ttttataaaa
     1381 attaatttaa gtgtagttaa attaagtatt aaactatctt atatattaca atatcctact
     1441 atcttatagt cttctggcac tttataatct ttgtctggag tacctaaatt ccattttaaa
     1501 tcaaacatag ttgcacttat tattgttgca aaataaagca taactatacc aatgtctatt
     1561 ttttcttcat ataaattagt atgaccatct tttctaacag ctaaaactac agtgcctcca
     1621 tcaactataa atctccatgg ttgtctattt aatgtagatg gagctaatct tgcatagtga
     1681 aatgcatcaa gaagtgctct ttcttctaag atttctatac ttgcaacatt tccccattcg
     1741 ttcatataaa caacatcttc tacacctagt ctagtagacc ccttatctgt tacaacttgc
     1801 atatcagatt ttgaataatt ttgcccagtt tttgtagcat ttaatacttt agcattactt
     1861 acattttctc catatccaat agctataatt cctgttacct ctttatctga attaattcca
     1921 agtttttctt ttaccttttt tccatcttca aaagtaaccc aacaagaacc aattccaagt
     1981 tctgttactt taaagattat actttctcct atgtaccctg aattttcaat gtaattatct
     2041 tttatgtcag ataatattat aatataactt ggagaatcta tcatatttcc tttgtaccct
     2101 gctactttat ctaaattatt aaaaacttca cttttattca taattcttac atcaacatct
     2161 atttctggaa ctagtttctt acaagattct gagtatttct taatttcact aagatattct
     2221 tctttaatat ctatgttctt atattctcta acagactttc tatttaatat gattgtttta
     2281 taatccataa ttttatcctc cccaaactag tattgtctta attatatacc cattaatata
     2341 ttattttact catttattta ataaataaag attacaattt tgtaattatt atttattaat
     2401 aatattccac ttttacatta ataatgacca gttgtgcaaa atccattgcc aaccaccaaa
     2461 taaaaacata ttataaaagt aatataaaat aacaaacaaa tcttaacaaa aaattaaact
     2521 ttttttacac gaaagaaggc ttcccgttgc cttctttatt tttataaaaa aataagctgt
     2581 ttctaaaatg atttcacatt tttgaaacag ccaagttatt ctataatgac aactataatt
     2641 tgaggatgtg agggagagtt gtcattataa atttagggta attatattat gttttaagct
     2701 agttcttcct atatttatat aatacatttt atatatgaat tttatatgag attggtataa
     2761 atgatatatg aaattacata tgtttatatt atattaaatt ttattattac attttccaca
     2821 acaatactca tatgtctcca cattatttgg aatatttaca aatacaaatt ctttacagtt
     2881 ctcacaatac tttaatatag atatagttcc acaagtgtaa cattcaaata ttacacaatc
     2941 ctcattttcc tctactattt caaacttttt actatgttca ccacactctg ggcacttgtg
     3001 attatttaca ttcataaata ctcctcctta ttagtatagt tacatttcaa aaacttaatt
     3061 aatgtattct atattactta ttatatattc tataataaat catattctaa tataataaaa
     3121 caaattaaat aaaaaaaaac cataaatttt aaatttatgg attaattctt gtaatgataa
     3181 ctctatttga ggatgtgagg gagggttatc attacaatag ggtattatat tatggttttt
     3241 aagctagtct ttctacacct catatgatac atatgctata tgaattttat atgattttta
     3301 cataaatatt ttatgaaatt taaatttttt agtcattttg aaatttgtaa aaagttctct
     3361 aaaaatccac agcatcataa acagccaaaa tttaagtaaa acatcataaa aaaatttgtc
     3421 caataatttt aacaagtcat ttcacctcat tataattatt gacacaaact taaataaaca
     3481 atctataaat cttatataaa tcttaaactt tacccttatt gatacacaat ttctagtttg
     3541 tcaattttat tttgcatctc taagaaaatt tcttcaacag aattgttaaa tatattgcct
     3601 aaatagactc ttttatcttt ttgaaatgaa taagatttta cactaccatc agaagaaata
     3661 tataaatgac tccactctgc tccatatttt gaaaacctct cctttgtatc tttaaacctc
     3721 atatcttcta tttttattgc tttttctctg tctcctctta ctacagtgtg accccttttt
     3781 attaaactgg ttatttttat atcttctttt aatttaatac gtgtatcatc attttttgaa
     3841 tatactttcc atatcacttt atgacgataa ttatctaatt cttttattaa gcttctatca
     3901 atagtaaagc tatctttagt taccacaatt aaatcaaaat ggtctaacat atattgaaat
     3961 atttctctca tacttttatt ttttaaaata tcctcaccaa taacctctat aaatctacag
     4021 cctttctcat acatgcagtt caataaactt gtgatttcag aaaaataatc atttgtatta
     4081 ttatctccat ctatttctat agtaaagtac aaaggagtaa tgtatgattt atttcctgtt
     4141 atctcaaata cacttgatag tgtctgtata tcatcttcaa acgttaagta tacattctca
     4201 taaaacataa aaaattccat aactttttct ttcatattat ggatatttct atatctattg
     4261 gataatatat ttattatatc gtcagtagta tttttcccat cacattcagc cagtataaca
     4321 atttgcttcc aacttaagct atccacattt ccgatacttt ttttatatcc atcttcttca
     4381 tcataaatag gaccaattat aatgttttct tttggccaca gatgaaccct agttccatct
     4441 tttaatttcg gatatttatt ttttacagac tcataaaatc tacttgtatc acccatgatt
     4501 ctttactccc ccccatagtt taaatattct atgctaaaaa tagcacttta tttttattaa
     4561 ttcaacaatt attcaaaatt ggtcattata tatgtttaaa tggatttaac atcaacctta
     4621 tttatatgtt attgatgttt atatatgtta tataaaatct tatttctcat acttcaatgt
     4681 aatcttaact atctctggtt tacaattaag cctaattaaa acttttgagc ctcctaagcc
     4741 tttatttaaa atcatagtgc tattatcttt ttcatatttt ccccagtcat acttaggaaa
     4801 gaactctcta tttggagata atacacctcc aacaaaaggt attcttatta tacctccatg
     4861 aacatgtcct gctaaaacca aatctgctcc ccattttgca tatttttcaa agaaaaatgg
     4921 tgtatgtgct aaaagaatgt tatattcatt ttcattaact ttacctagat tgtcttctac
     4981 aaaattgtca cttagttcta attttttgtt attagcaaat aaatatggat agcattccaa
     5041 tggtataatt aaaccatata aattaagatg gctattacct ctatatattc ttacagattc
     5101 attttccata tttattattt tctttgaata tagttcctta aaataatcct tatatagatg
     5161 tttatacttt tttattaaag atttttgttc atgatttcca attatatgat atacttcata
     5221 tttttcaacc aaattatcta ttaaatctaa tgcaacttga aaatttttat tttctccatc
     5281 tactaaatct cctgttataa atataacatc tgggtttaat gattcaattt tatctatcaa
     5341 taccttatta tttttatcaa acaccttatt atgtaaatca ctcacttgaa ctattttaaa
     5401 gttatcaaat tcctttggaa tttttttgtt gaaaatagta tattctgtcg tttctatatg
     5461 ctgagtttca tacttataca gaatatacaa taatattaat actaatactg tcattgaaac
     5521 ctttataatc atattctctt cctctttatt ctattttgtt aatattatta taatgctttt
     5581 atgttttttt taaaacaata acatcatcac atacatataa atcttttcga cattataaaa
     5641 ataagttatc cttttctaaa attttcattt attttagaaa aggataacta taaaattatc
     5701 tataaacctc tatattgtga aatatagttt ttcctttatt taaattttta tatatatgct
     5761 ctaaagttcc atcaaaccta atagaatgtc cagactctaa aatataagtt ttcccatcca
     5821 cattaaggtc aagtttacct tctattacga gtacatactc ttctaaaaca taactatgtg
     5881 tagatgaaac atgagtacag ttttcctcaa gctcaattat aaaaagttca aaccttcttt
     5941 gagggctaaa agggtatatt ggatataact tcatgttccc tttttgctca acaataggct
     6001 ctacttcttc tttctttata acctcatagg tatttgtatt ttcatttaat agtgtagtaa
     6061 aatttatttt aaatccagat gaaattttcc ataaagtaga tactgtcgga cttgattcac
     6121 ctctctcaat ctgtccaagc attgctttac tgacacctgt catctttgaa gctttgtcta
     6181 aacttatgcc catttcttgt ctcaataacc ttaaattttc tcccacatta ggaaatttat
     6241 tatccatgtt aaaatcctcc tgatagctat ttgtattcta tgatttttac tcctgtaagt
     6301 tctactatct cttttaattt taacatatga ttgttacaaa tttcatcaaa ttcatatata
     6361 taatcttcaa gccctaatgc cttatattta tcgattccaa atttatgata tggaagtaac
     6421 tccatcttgc ctcctggaac attttgtttg acaaataatg cagtatttct tatattttct
     6481 tcactatcat ttactccata aataattggc actcttataa ccatagattt attcaacttt
     6541 gaaagcctac atatattatc taatataatt ttattactta ctcctgtata ttctttatga
     6601 atattatcat ccatagattt tatatctaca aatatgtggt ctattttctc aaatacatca
     6661 tttaccttat tccaatcaaa atatccacaa gtttcaattg caatatttat gcctttatca
     6721 taaaaaatat caactaattc tcttaaaaaa tcaatctgaa gtgtaggttc acctccagaa
     6781 aaagttatcc ctccattaga ttcaaagaaa aatataaaat ccttttcaac ttcctctact
     6841 atttcttcaa cactcatttt ttcggtcatt atacaaatgg catctttaag acataccttg
     6901 atacactctc cacataaatc acatttatta tttatttgcg ttctatcaaa tagtgaactt
     6961 atattttgag gacaaacatc tatacatcta ttacaggata cacatttatc tttcataaca
     7021 cctaatttta ctatgttact ccaggaatct ggattagaac accacttaca ttttaattta
     7081 catccttcta aaaatatagt agtccttatt ccatctccat catttactga aaaatgttgt
     7141 acttttacaa ctctaccttt tttactcata atctacctca taattaaaaa ttgctatgtg
     7201 aatttctagc tattatagca tcttgcattt ctcttgataa attaacaaat tgagtactat
     7261 atccagcaac tctcactaat aaatctttat attcttctgg attttcttga gcttttaata
     7321 gagtatcatt tgatatcgta ttaaattgta catggaatcc tcctaaagca aaatatgatt
     7381 taatcataga tgttagattt ctaagtcctc tttcagtttc caccaaatct tgattcagtc
     7441 ttaaatttag aagtgttcca ccagtatgaa catcatgatt tatttttgat gcagatttca
     7501 tggctgctag tggacttgtt gtatcagtac caacaaacgg agaaactccc tctgtcaaag
     7561 gttttgtcgc ttttcttcca caaggagttg cacctacgat ttttccagtt ggtacatagt
     7621 ttgatatacc cataaatgca ctattaaatt tagagccaaa aatatccttg tattttctag
     7681 tttcagtata ataaaaatca gaaacttcaa ttgctaatga atctacataa tcattatcat
     7741 ttccatactt aggaacatcc aacgcaagtt ttcttaattc ttcataacct tcccaatcat
     7801 tatttagtgc ttttgttaat tcttctagcg tagtcacctt atcctcaaat actaattttt
     7861 ttatagctgc caatgaattt gaaactacac ctaaccctat accagttaat acaggaccta
     7921 tattatattt tgctcctcct ttacttaaat caactccctt atccaaacat ccatctacac
     7981 aagtagataa aaatggtctt ggtaccattt ctttatgaat ttgttgagca acagtagttc
     8041 ctataacaga actttttaca agatatgcta attgctttaa aaactcatcc ttaagttctt
     8101 caaaagaagt aaattctgtg atattttttg tatctaaccc cattttttct ccagttaatc
     8161 tactcttacc ttcattcata gcatattcta aagctgctgc aaaattgata tttacggctg
     8221 aagtccactg tcctgttttt ctaaaatgag gaactacaca accacagtta ctccaatctc
     8281 tagcatcttc tgcatcatat ccatcttgta ataacatttg tgcccctgca ctatcgctgt
     8341 gtatagcagg gaaaccagtt ccttgtttta ccaatttagc aactgccatt acgaaattat
     8401 ctggtgctcc ttgactcact ctaacactta atcctggctg gtgagttttt acactttctg
     8461 tagcctttaa acacatatat gagatatcat tcgttccatc agtaccattt ctctttttac
     8521 caccaacagt aagattttgg aattgattat atcctgcaaa gtaatttgct gtatttgctg
     8581 atatagtcca tacccattct gataacttaa tccaaagtgc ttctatcaat tcttgagctt
     8641 ctgattcagt tattttacct tctctagcat cattttcata atatggatac atatattggt
     8701 caaatctacc taaatttaat gccaatggat tttctgatag aataccacct aattgaacaa
     8761 accatacaaa ttgtattgct tcataaaaat tagttggagg gtttgctggt acattcatac
     8821 atatctctga aatttttata agttctaact ttctcttttc atctgtttct tgcttactca
     8881 tctcttttgc aagatttgaa tatctttgtg ccaatgtcat aataccctct gcaactatag
     8941 aaacagattt gtaaaaatct atcttttcaa tattttctga aactgatata tctaattctt
     9001 taattttttt atctgcatct tccttaatcc ctttatatcc ttttacaaac aatatatctt
     9061 gataatctgg tgttacttca ccaactgcct gtctccactt agaatcatta tctattattc
     9121 ctgtatctac taatatctta gctgtatctt ctgggattct tgctaagaaa acttcttcca
     9181 aagattttcc cttccaatat ggaaatattt cttttcttat aaattctatt tgttcttttg
     9241 atattacata tgggtcttga gttcttttat caaaagtatc catttctttg tctacccatt
     9301 gccaagaaaa ctctggtgtc aatatccctg ttcttctaaa ttttcctgct gtacctacta
     9361 ttaattcatt ttcaaagatt tttataggaa gttctttaca tgtctccaaa aaagctttag
     9421 ctcttcttat acagatagct tctccctcag tttttttatg tgattctgta aaaattctag
     9481 ccctttcaca acatatcata ggtttagaat taatatattc ttgtctaaat gcttctattc
     9541 tattagtttg actcatttaa aatccccctt tggataatat atactacatt tttgtatgtt
     9601 ataacatact ttaattatat tatactgtcc tttagcataa aatggaagca aaataacttt
     9661 ttttttcatc taccttgaac acctatatat taatactttt tttgttaaat ttataaaatc
     9721 taaaaaaatt ttctatgata tattttctat attgttatag ttatttctaa tatactaatg
     9781 atttgaaata tcaaatttgt atactagact tagttttaaa accttagttg attgtttaac
     9841 tttgttaact tcaacatgta tatccttcat ataaggaagc tccacaacac ctactccctt
     9901 attaaattct tcttcaacca tagaaaaaat ttctttctca gttaagttcg tattagtcaa
     9961 tgatttaaat aaattgtata taaatttaat atatttatca ctctcagata acttatcatc
    10021 ttctggatac tctttagcac agagaatatc tattgttact tttccttctt tatcgtcttc
    10081 agacatagaa tagaataaac ttcgaatatt tttttcaggt ggtttttcag caccatttat
    10141 agatactaca aagctaccat ctgattttga gtcagtaata ctataaccac tgtctattat
    10201 agctttcgaa aatctttgag ataaatcttt ttttacttct tctctacctt tcgcatcatt
    10261 aatcttttgt gagtaattat taaatagctc gatagattca tcttttttat cttcaacttg
    10321 ttctttcaat gaatctttac taatttgttc tgagctactt aagttcttat cagatacagg
    10381 agacatacat cctgccatca atgctaagct aagatttaaa gccacaatta ttgccactct
    10441 atttatattc ctttttactt tcatacacaa tcctcccatc acactaaccg ctctaaagta
    10501 ttattcattg cattagaata tatttatatt ttctttatat taaagattta ttattcaata
    10561 catatcacct tcctatccaa ttcttataac ttactattag taaaattata agctccttaa
    10621 tacagaaata aggatgttat cttttgttat taaaagataa catccttatt tttttgtatc
    10681 aatacatatg tcccaactgg taacttaatc gccccaactg tcagtatctt gctccaatgg
    10741 tatatatata tttagtataa atttatcgat actattttca aataataatt ctccattata
    10801 tttttgtaaa gaagatttaa tactctgaat acctattcca tgtacgaact tatccatttt
    10861 gtttgttaac aatatatttt tattaaaagt aagctgattt agtttactat tttcacattt
    10921 aagtacaaaa aatgactttg ttatagttcc tttaattcta atatatttat taatattttc
    10981 atccactatt ttatcacatg cttctattgc attatccaaa atattagaaa atatgcttga
    11041 cacatctata ggttttacaa aatttacttt tgaaaaattt atagagcaag taaatttaat
    11101 tccttttttt aaacatattt cactcttttc gttgattata atgtctaata tcatatttcc
    11161 tgtattatag atatatttaa atgtttttat ttcatctttt aaattgttaa catattcatt
    11221 aatttctttg ctattgtttc taaggttgtc tatacaataa atgtggttat ttatatcgtg
    11281 atatagtttc ttaactcgca tatgagattc ttgaatactt aaataatgcg catattgcat
    11341 atctaactta ttttttatta attcagcttg agattttaat ctattctctt ttattatttc
    11401 tttaaacaat aatataagaa atatactact agctcctacc aactttggat taacaaaatt
    11461 atcaaaatag tatccttctg aatgtaatga cccaaaccgt tctaattttt ttaatacata
    11521 atttacaagc atattcattg tattagctaa taaggcaaat aataaatata cataatttct
    11581 cttatcttgg ctaattaatt ttatagactt cctaaaaaag ctaaatacaa gatatataag
    11641 aataattatt aaattactat atatctgaat ttttatagat tctaagtgag gtactaataa
    11701 ggattcttta gatgaaataa cattacctgt aattgcaaaa aatagtattc tatatataac
    11761 agcatccaaa catttataat atacactaaa tagataaaag aaaatggaat acttaatata
    11821 atccatctta taaaaataat tatagtatcc tccccaagca ataaatgcta atatataaaa
    11881 taaatagttt atattactaa agtaacttcc tccattaaat aatctactta tctgaattat
    11941 aatataaata agaaacaaga caatatagaa tactattttt tcattttttt tactatcctt
    12001 tttataaatt aaaattccgt tcattataga aaactgtata aatatagata aaactactaa
    12061 tagctcattc ataaaattta gcatattgta tcaccaagaa attttaaaaa tttctcctta
    12121 acttctttat atctatatct actaataggt acctcttctc cactttccag tattgcagca
    12181 ttaggcttta tattttcaac atatcttaaa tttactataa aacttttatg gcatcttata
    12241 aatttatatg gatttaattc tttttctatt ttacctaaac tatattttat atcaaaattc
    12301 ttgtttattg tgtgtattag catatctttt ttttgaactt ctatatattt tatttcattt
    12361 aaataaatct tatatgtatt agacttattt tttattgtaa tatgactttc cttatttatt
    12421 tcaatatctt tgatacaagt tagcacatgt ttttttagtt cttccaactc tattggcttt
    12481 agtaaatacc tataagctct tacctcataa ccttcttgca cataatctat taatgaagtt
    12541 acaaatatta tttccatttc atcgtctact tctctaatct ttcttgcagt atccattcca
    12601 tttaattttt ccatttttat atcaagaagt aatatatcta tatcttttaa attactttcc
    12661 agtaactctt ctccagatat atacttttgt atctcatatt ctatacttat atcttttaat
    12721 atattctcaa ggtaatcttc tattaaaagt tgtgtttctt tttcatcttc acaaactgct
    12781 atcttaatca taaaatgcct ccttacataa tttctagtta atacaattaa aatatttata
    12841 atatatttaa agataatttt gtatatctga tactttagga taacattttt aaattatttt
    12901 tatcaatact caatccaata caactattat ttgatatttc aatttaaaga cagaaattac
    12961 tataattaaa aacagtactt tactaacaaa agaagatgtc ttaaaatatt aaaaaaataa
    13021 ttttaggaca ccttctttta tttatttaaa ctttttaagc cttaaataat acattaatct
    13081 cctatataat tgtactgcat ataaaatcaa caatataatc tgtagtacaa tagcagttac
    13141 agatgctcca taaccactat ataaatttct tatttcaata ttcaataaat atataaatat
    13201 tactactaac ataaagaata taaacatatt cataaataaa attgacttta ctttgttttt
    13261 tttatcagaa tcactatata atttaggagg tatgtctttg tattctttcg accatatata
    13321 ccatgcagat gtacccataa aactaccaat actcctaaat acaagattcc agccatttga
    13381 tttatgttca tcaaaataat tattctctac atcatagtag taatcaacag tgtattttat
    13441 ttttcttgga cttcccttat gaaaataaaa tattcctcca aatttattta tcttatataa
    13501 attatatcct ttagcttcaa tatcttctaa ccatttctct atcttatcag gtgaactata
    13561 ccagtcaaat ttgaatttta ttttcttttc catagcactc attctccaaa atgatatttt
    13621 taaatagctt aaaaatctat ttaaataatt atatcatttt gatactaatt ttaggttaca
    13681 atcatatatt taaaaagcta tttaaatagt aataaactaa tagccataac acccattcct
    13741 gcaatcaatc cataaataga taaatgatgt tccccgtact ctctagctga tggcaataat
    13801 tcatctagtg atatgaatac cataattcct cctacaattg caaatacaat tccaagagtt
    13861 atatcattta aaaaatttct aagaagtaca tatccaataa tcgctcctaa aggctcagac
    13921 ataccagata aaaaggaata ataaaaagct ttctttttat ctcctgtggc ataataaact
    13981 ggtacagata cagatatacc ttctggtata ttgtgtattg caattgcaat tgctatcggg
    14041 atagctattg taacatcatc aagagctgat ataaatgtag ctaatccttc aggaaaattg
    14101 tgaattgcta tagccattgc tgtaaatata cctgttctca aaagagactt attttttttg
    14161 atattctttt cttctatatt ctccattttc tttatttcat gtggattttc ttcttgtgga
    14221 attaacttat caataacagc aatgatagcc atacctataa aaaaagctat tacagttgac
    14281 caacttccta atttctctcc catagatttt gtcaaagcat cttgtgcttt aggaaaaatt
    14341 tctatcatag aaacataaat cataacacca gcagaaaatc ctaagcttat agaaagaaac
    14401 ttagtatttg tttttttagc aaaaaatgct atacaacttc ctataccagt agataaacca
    14461 gccaataatg ttatcaaaaa tgcaaatatt acattattca aataaatcac cttcttttta
    14521 attgatattg attatcattt tctttatgct atcatatata ttttatatag tcaatattta
    14581 taaaaaatat acacaataaa aaagcagaga tataaactta gtctctgctt tttctttata
    14641 gtatattaaa attatttatt taagaaatcc actgcatatt gcacatataa agcagttcca
    14701 atttctagag catcttcgtc tatattaaag catccatgat gatgaggata gcaagcacct
    14761 tttgactcat ttctagctcc tacaaatgct agtgcaccag gtgctatatt cataaactca
    14821 gctaaatctt ctgctcctgt tactttttca aatagtgtta tacagtcttc tccaaatagc
    14881 ttaattgctg catctgtagc tatctttgaa cattctttgt catttataac tgctggtgtt
    14941 aagtacccat actctaattc agcatctgct ctatatgctt ctgctgtaga ttttgctata
    15001 cgttctaaaa ttccaggaat ttgctttcta agctctggat taaatagcct tattgtacct
    15061 tctaagacag cttcactcgc aattacgtta aatctagttc cagaatttaa aactccaaca
    15121 ctcacaacta atggttccag aggacttact tctctactaa ccatagattg tagattcata
    15181 actattgctg aacttgcaag cactgcatca acaccttgat gaggaagtga accatgacca
    15241 cctctacctt ttacagtaat tttaaagaaa tcggcagaag ccattcttgg tccttcttct
    15301 accgatatag tcccagattc tacatcagtc caaagatgta ttccaaacac actatctaca
    15361 ccttccatag caccatcttg aatcatagct cttgcacctt taccaacttc ttctcctggc
    15421 tgaaaaaata attttacagt tccattgata ctatccttaa tatcatttaa cacttttgct
    15481 gcccctagaa gcattgatgt gtgaccatca tgaccacaag catgcataag tccttcattt
    15541 ttagatttgt attctacatc agtgcattca acaacttgta gagcatccat atcaccccta
    15601 agtgctacag tctttcctgg attagctcct tttatagttg caattacacc tgtacctcca
    15661 gctgatacat atggtatctc tattttatca agttcttctt taactctctt tgatgttcta
    15721 acttcttcca tacttttctc aggattttca tgaaattccc ttctaagctt aattacatag
    15781 tctttataac tctttgttat ttctttaata tccattccat acctccaagt agtacacatt
    15841 cttcttattt aaaatacttt ttatcactta aatactatat tagatttaca aagatacctg
    15901 ctattactac agaagctatt gtaactgtag taaatcctcc aacaagcatc tttggaagca
    15961 tcttatccat taaaaattct ttttcttctg gtgtttcagc caaagccttt actgcctctt
    16021 cagttaatat atagtttggt ggaaaaccat aaagtgatgt aagcgaaact gaaaaagcca
    16081 tttctttact ttcacctaaa agcttaccta ctataataga gaatattccc atacctatta
    16141 caccaaatat tatgattcct acaagaggaa ctgccatttc aacaagcatt gctggtgtag
    16201 cttttgctaa tcctgcaaat ataaaagcca tcaatgatgt cattaaccat ccaaatgaac
    16261 cagatttatt aagtggtttt ctatcaacta ttccaagttc tgatgctatt acaccaaata
    16321 ttaaacatat aacatatctt gatacaactt catttgtaag atttgtaaat tgtactgcta
    16381 accatgctac aaatcctaat ctaagtatta tcatatatgt cgtatcatat ttttctggta
    16441 agtctggaat tatcttaaat ctgctttttt gttcaggtaa atccttaact ttttcttcat
    16501 ctgtatttac agttactttt ccacttctaa agtctgataa aagtcttttt ccttcttttt
    16561 taagcattaa tgctgtaata ggataacccg caaatccttg tgcaacatac atcaaaattg
    16621 caagtactga taaacttgca agaccttttg ctgtagcagc atctgccatc attatagatg
    16681 caactagccc tccagtaaga ggaggtgtag ctattactgc tgtatcccat ccaaatacta
    16741 ttgtccctaa tgccaaagca cctacacata ttcctactat tcctgccaag gctattacta
    16801 ttgttttcca ttgacctgct aattccttaa tactcatcat agttcccata tgagttatta
    16861 acaaaagcat acctaattga gctaatggtg ttcctaaagc ggctaagtcc acaatatttt
    16921 ctgggaaaaa agtccagaat cctactataa atataattgc tgatacaaaa accgacggaa
    16981 caaaagcttt agtttttgct gaaactatat ctcctatcgt cagtgatatt aaaactagac
    17041 aaaaagctgt tattgcctcc atacaaaatc ccccctcata ataaatgtat ttttataaaa
    17101 tgtatgtata gttatccatt attttactac ttttgaaaaa catttcaagt caaaattcga
    17161 tttttttcat aaaattcgat aaattccata aattaaagtt agttttctaa attattcttt
    17221 ttgtaaaaac gttatcctga ccattggcta tttaactcta ttcttacaaa gagaattaaa
    17281 tgtaatttgc aaaaaataaa acataggata cattttattc ataaaacatc tcataaatat
    17341 aaaatgaatt gtctttatga atcttaatat atcctatgtt tgtaagttta atgcgtatgt
    17401 tagctatggt gtaaaatttg tatactagtt attgtgtaac ctactatata ctccattttt
    17461 gtttactaac tcttcatgag tacctctttc ttcaattcct ttatccgtca atactatgat
    17521 ttcatcagca ttttttatag tagtcaatct atgtgcaaca actaaatttg ttctatcttt
    17581 acttaattcc tctaaagact cttgaatttc tctttctgtt acattgtcta aagcagatgt
    17641 agcctcatcc aatattatta ttggaggatt ctttaaaaag attcttgaaa ttgaaattct
    17701 ttgtttttgc ccacctgata attttacgcc tctttctcct atataagtat catatccatc
    17761 tggcaaagtc tctacaaagt catgtattct agctttttta gcagcagcta tcatttcttc
    17821 atctgtagca ttagggttcc cacaaagaat attatctctt atagttcctg taaataaaaa
    17881 tacatcttgt tgaactatac ctatattttt tctcaatgat tttaaactaa catcttttat
    17941 actctttcca tcaattttta tatcaccctt atcaaattca taaaatcttg gcaatagatt
    18001 acataatgta gtctttcctc ctccagaagg tccaactaat gctatagtct taccagcttt
    18061 tatagataaa cttaaattat ccaaaacttg agtcttatct tcatatgtga aagatatatt
    18121 ttctatctca atgtcacctt ttacattttc aagttcaata ggatttttag cttctttttg
    18181 atggtcttgt tccattatct ctatgaatct tttaaaacct gtcataccat tttgatattg
    18241 ctctgtaaaa tttattaatt ttttcatagg ttgtataaac atttttacat atagtatata
    18301 agcagcaaaa tcacccaaag taatttttcc tatataagta aaataaccac cagctattac
    18361 aactacccac tctaagatat ccatgtataa attcatacca gagtaatatt ctgccattac
    18421 cttataagaa tcttgtcttg ctcttctaaa taatttattg cttcttacaa acttatttaa
    18481 ctcttctttc tcagtacaaa aagacttagt tactttcata cctgctatac tattttctaa
    18541 agtagcattt acatcaccta tagtaactct actcttcata aaagcaatat tcatcttatc
    18601 ttttttagct attgcaaacc aaattataaa cggtaatata gcaaatatta taagcgtaag
    18661 aggaatattt atatctataa gtataaagaa tgaaccaacc aacattacta tagaaatgaa
    18721 taaatcttca ggaccatgat gtgcaagttc tgttatatcc attaaatcat ttactattct
    18781 agacatagtt actccagatt tgttattatc aaaatacgta tttggaagtc tttgtaagtg
    18841 tgtaaatacc tcatttctca tatcagcttg catatctaca ccaataacat gtccccaata
    18901 ctgcataaaa tagttaagcc ctgcttttgc tataaatata agtataagtg ctactgcaaa
    18961 aactactaac attcttaagt ttttatttgg aacaacgtca tccattatat ttctagttat
    19021 cataggataa actaaatcac ataaggaaaa tagaaaagct gctataaggt ctagtgtaaa
    19081 aattttctta tatggcttat aatatttgac aaattgtttt atcataagta ttaaatccct
    19141 cctttattag tacttctata tagtacttct aaaaaatacc cataaattag tataacataa
    19201 caataacttt aaatctttta taaacttaca aagtaataac aatctttttc ataaaattga
    19261 agatttatat catatagttg tatacttgaa acagcataaa aagagctata tctctatatt
    19321 taaaatttta taaagataca actctatatt taatactaaa ttttttttat aaacacaaaa
    19381 ctacttcaat gcatttaata tctttttatc taatgctgtt gtatttatac ctgttatata
    19441 tcctgttgcc tttattgtag gtatatcata agttgttggt agtatagtag tagatacgat
    19501 taaatcagca ccttcttgtt ttgttgaaca ttcagcaatt ttacattgct caacttctgc
    19561 gtgtatatta ttttctttaa ccaatctttc tactctgtca caaactattg ttgatgttgc
    19621 gattcctgct ccacatgcta ctaatatttt tttcatatat tgtcctccta caatttcttt
    19681 aatattttat aactcaaaat aaaattgata aatttcttat atctttattt atataaataa
    19741 ttatatcatt ttatataaga agaacatctt aaaattattc ttttaatatg ttttttattt
    19801 caacattgct tatacttaaa atttcatttt ttatacaaga ctctatgtac tttgatatat
    19861 ttttataatc atttaaatta aactgttcaa tattcaattt atattctata tcactgacaa
    19921 tacctatcaa atcagactta ttagataaaa tatcctcata cttacctttt ctagttactt
    19981 ccaatctttt aaatctataa tttttatatc cttctatgat aatcaagtcc atatcttcat
    20041 ataattgaat cattttatat aatggattct tctcttttag ttcttcaata tatgctactc
    20101 gtttatctga tattatacag acttttctcg ccccagattt tctatgttta taagtatctt
    20161 ttccttcctc atccatatca aaatcatggc aggtatgttt aataactcca acacgatacc
    20221 cttttttatt taattctttt attatgcctt caatcaacgt agttttacct actcctgaat
    20281 atgagacaac tgacaatata ttatacataa aattgtgtcc tttcataaaa ctatctaata
    20341 acaatctcca ctaattgatt ttcttttatt gtctcattct ttttaatctt aatcatacaa
    20401 ttacttctta gagttgcaga taatctagct tttgcatctt tcttatcatt tagctctaca
    20461 tacaatttac cttcacttgt atatagtttt ccacttacaa atctatcttt atttgaaagt
    20521 ttattaaatt ctcctttaaa tatagctttt atatgatgta ttcccttctt atatgttaaa
    20581 tactcaataa caggaactat cacattatca aataccacta ttgctgcatg tggattacca
    20641 gaaagaccaa ataatagctt ttctcccaat atacctacat aacaagctcc accaggttta
    20701 atgtttatct tccaaaataa ttctgttgcc cctatttttt tgaatacatc tggaattaaa
    20761 tctttatctc caacagaaac accacctgtt gtaattaaaa aatctacact ctgtaacata
    20821 ttctttacct ttttttctaa ctccaatata tcatcagaac aattgcatat tgtatctgcc
    20881 tgtattccaa gttttaataa tcttgaatat aaggtatatc tattactatc gtatatttta
    20941 ccattctcta aagtctcatt tatatcttgt agttctgtac ctgtattcaa aattccaact
    21001 tttaaatcat cataaacagt tactttggat attccaagac ttgctaatac tccaatacca
    21061 tcatatccta tattctgatt tttattaata atcaattgcc ccttttttat atcctcaccc
    21121 ttaaatatgt aattatcgta ttcctttaat tttacattta aaattatcat atcacctata
    21181 accgttacat cttctttttt tattacacag tttgcaccct gtggtatctt tccaccagtc
    21241 attatcttca ctgcttcatt tttatttaat accttattaa atacttctcc tgcatacaaa
    21301 gtccctataa cctttaaatt tctattttca gaatttgcat tatatgcata accatcatag
    21361 ggtgacctat caaatggagg actatcaatt gttgaatata tatcttctgc aactacttta
    21421 tttactgcat ctataagatt tactatcttg accctgttta ttggtaaaac atttttttca
    21481 ataattttta tggcatcttc taattctatc attttaaata ctccccaata tattttttat
    21541 ttaagatatt tctatgtaat atattataga ttataaaagt tggaaatact tttatcttat
    21601 atttaattat aactaatagt tatctataag tgcagttcaa attttataaa tctaataaaa
    21661 aaactgttcc cagctagaaa tttccttcta tcttgaaaca gcatcatatg ctttagaata
    21721 ttttattcta ataaagttcc ttttattatt tttacaattt tatcactttc aatagttttt
    21781 atagatttta ttttgttatc cacaatactt attgtaagta cctctttttt taatattttg
    21841 ttatcttctt tcaatctata actcttaact actttactac cttcatgatt atatgctgtt
    21901 aaatcataat tcgcatctcc tacaatttca tttattctag actcttcaac accaacatac
    21961 tctcccaggt caatacgtct aaaattttct agtgatacag ccgatttttc atggtcactt
    22021 ttttcaatca caacactaga ttcagcatca atgactgata taccttgtga tgagaaagaa
    22081 ttggtttcaa cattgacaac tttattttcg ttaatatcaa catataaagc acttccatct
    22141 agttcatcat ttccagtata ttttggataa actaatgtca tataacttga tgtatttata
    22201 tcttgcattg tattcgattc ttttaaaact ttaggtttta tataataaaa tgtattataa
    22261 ggtataccca tattctttat aatgtactta taatctttat tcataatttc attaacatca
    22321 ttttgaactt ttgtcatagt tttttgctgt gttgctttac ttgctacttg atttttctct
    22381 ttagtatttt ctttatgaca tccaacaagt ataataccta aaatagttac acaaactaat
    22441 gaccttttta acttcataaa tcaatcctcc tttcttaaat tttatagtat ttatctatat
    22501 ctcaatttac agttatctct tttttcaata attatattta ttatattata aaataattat
    22561 ataatattta tttgaattta ataagattaa gaagctaatt gtaatttttc tgtaataaaa
    22621 aacactttaa agttgaattt tgaaaattca atttacgata tttttattgt aagtacctta
    22681 caatattata ccaaattatt cctaaacaat caaaaactcc atgaatattt aaaggttttc
    22741 tattttaaaa atcacctttg actattaatt tatcaaatat gttataatag ttttagataa
    22801 tttaaggagg attaaataat ggattttaaa caacttgaag tatttgttgc tgttgcaaaa
    22861 catcagagtt tttcaaaggc cgcaagagaa ctttttttaa ctcaacctac tgtaagtgca
    22921 cacatacaga atttagagag agagttggaa actgttctta taaatagaag caacaaagtt
    22981 attacactca caaaatctgg tgaaattctt tatgaacatg ctatatatat attgaataat
    23041 tgcaaacgtg caatttatga tataaaagaa tattcaggaa aaatagaagg tattatagat
    23101 atagcatgta gttccatacc tgaaacttat attttacctg attttatgaa gagtttttct
    23161 atgagttatc cagatgtaaa attttccata agccattatg attctcaata tgctatatca
    23221 gaaattttaa atgaacgaat aagctttgga ttagttggtt ctaaaatcaa taatcctcaa
    23281 atagagtacc tagacttatt agatgatgaa cttgttctta tcactccttc agattttaaa
    23341 attgataata aaaataattg tatcgatata ggagaattgg cttatctaaa ttttattatg
    23401 agaaaagaag ggtcaggtac aagaaactta atactaaaca cacttagtaa aaataacttt
    23461 ccagtaagta aattaaatgt aatagctcat gtagaatcaa atgaggctat taaggaaatg
    23521 gttagattag gtcttggagt ctcctttata tcgtacattt cagctattga ttatctcaat
    23581 gcaggtaaaa taaaatgtta taaaataaag ggtgttgatt ttactagaaa atttttcttt
    23641 atttactcta aaaagaaaac tttttcccca ttagaagata aatttctaaa cagattatgt
    23701 gagtatttcg aaattataat ataataactt taataaagta gtgcttaatt ttgggtttat
    23761 aactaattaa taagctaatt ataactatca cataaatgct tataaattat tatgaaccat
    23821 ctttcttttt tatttaattt agtcaaaaaa tccctcgttc aaatgaactt gggatttttt
    23881 tatttatact attccaatag cagctaatac tatacaaata atcataactg aaattaatat
    23941 cgtagttgtc tttacctttt ttcctaataa ccaatacata ataccgaata tcatagctgg
    24001 aagcatacat ggcattattt ggtctaagta agtttgaata ggctctgccc attctccact
    24061 acctacatta acactaagtt ttaacattac attagatgca gtcatacctc caattaccat
    24121 aagccctaac attgaagctg cttccataac ttttgaaact atacttgaac tactcacatc
    24181 tttaaagaat tgagttccat acttaaatcc tttatctaga catatatagc gtataataaa
    24241 atgaggaaca tttattatta aaaggaataa aatcggtccc attatgttac cctgagaagc
    24301 taaagctata ccaactccag ctgctataag ctttaatgtt ccccagaaaa atgaatctcc
    24361 tatcccagaa actggaccca ttaaacttga tttaacagca tttatactat tagcatcaaa
    24421 ttctttattc tttgcatttt cttcttccat agctccagat atacctacca aaagagtact
    24481 tatatgagga gtaactgaca taaattctaa atgtcttttt aaagccttag atttcttttc
    24541 cttatcatct ttatacaatc tacggattat tggtgccatc atgtatgcat atgctatatt
    24601 ttgcatacgt tcgtagttcc aagatgaatc taatgtcaat gaccttctaa agcatttcca
    24661 aaagtctttc ctcgttattt tactatcggg tgaaattgta tctaacttct tagaactcgt
    24721 tgtcatctac tattacctcc ttctctccta caaagttaac atatattaaa gctattgcta
    24781 atccaaacaa tgcaactcct gttactgtta tgtttaggta agcaaccaat ataaatccta
    24841 ataataaaaa tgctactgtt ttcttattaa gcatcatcct agctaacatg gcaaaaccta
    24901 ttgctggaat cacgcctgct gcaacatcca ttccatgagt tacatatgat ggtataaatg
    24961 aaagtatttt ttctattact ggaacaccta atgaaaatcc tatacctact attattgcta
    25021 atattaattt atttcctata ccaaataata attgcacacg ttctacgcct tcaatattcc
    25081 cctcttctgc ataacggtca gcaatatgag ctccccatgt aagtactact gtcattaaga
    25141 agttatctat aagcaatact agagttgcta taggaactgc aagtacaact gctgctgata
    25201 catcttgacc cgttaatatt acaaacgctg tggcaataat agaaccagac acaaaatcag
    25261 gaggattact tgccccaaca ggaaatgcac ccataaatac taattctaat gttgaaccta
    25321 ttactatacc tgtatgaaga ttacctagtg ctaaaccaac aagtggtgca acaactatag
    25381 gtctattatg cattgtagaa cctacaaaac tatgcatata tgcaaagaat gcaattagag
    25441 ttatcaaaat aacttgtgta aattccattt tcatccccct aaaattatat taatttcatt
    25501 acatttgtag catcatcatc aggaactaat tgtacaaata catttacgcc ttctgaatct
    25561 aactctttaa tcatctttat atcatctttt gatacatgca ctgctttaga tatattttca
    25621 cgattatcat ctgattttgt tccaccaagg tttatagatt taatagcttt tacctcttta
    25681 gcaagtctat aaacatcttc tacagattca caaagtatca ataagttata tttatcagta
    25741 acacctgagt taagtgcttt tatagaatct gctatgcttt tcattactaa cttaacatta
    25801 gatggtttag ccattttaag ccctgccatt cttaattcat cttttaataa ttcatcactt
    25861 gctatcaata tacagtctgt gcttaaaaac tttgtccatg taaatgctac ttgtccatgt
    25921 ataagtctgt ggtctactct tactaattta atcattataa ttcctcccta atttttttga
    25981 tttcatccat gaatgttttt acgtgttcta cactaacatt cccaaaatct ttgtaattat
    26041 ctttaaagta acttcctatt attgccatat ctcctaattc aagctgtttt tttgcatttt
    26101 ctaatgtaac tccagcagct ataaccaatg gaaagtctcc taaattttta cgaaacagtt
    26161 ctattttttc cattgatgtt tcttgacctg tagcattttc tgtaactgct atagcatcac
    26221 atcttgtcat tcctattttt aaatcttctt caacgtcatt ttctgataat actggctgat
    26281 atttaaaacg tacgcctcca attaaatacg ctgggcattt tgaacgttgt aatttaaaaa
    26341 attcttctaa agtagcttcg tctctaggtt taacatgacc tactacagaa tcaacctgta
    26401 tataactagc attatatttt gttgccagtt caaatcccat agtatcaaca tttaaacaat
    26461 ttactccata tggtattgac aaattagctt ttgatacata ttcaagtatt ctttctaaat
    26521 cataataatt accatagtaa ttttctaaca ttattccatc tacaccattt tcttcaaaga
    26581 ttgtaatctc ttttttagct ctttcaaaga tatcttctgg agtatctcct tttaagtgta
    26641 tcattgctat aattggtttt ttacttttaa atactgataa tattcccttt tttttcataa
    26701 actaaaatac ctcttcttct tctttaattt gtaatgtcac gctttgtatt cctcttctag
    26761 attcttccac taattgttct atatcttcaa ctgttaattc atcagaaaac cttgtaagaa
    26821 gttctaatac catattcaaa ttcattccag aaaataactt aacattatgt agattagtta
    26881 atcttaacat tgctgtacat acacttgctc catataagtc agataaaatt atatattcac
    26941 attctatatc ttctgatatt gttttttcta attctaaagc aaaatctgaa gcactttgtc
    27001 cagggtacaa accatacgaa ctaacacaat ctgctaaatc tcctactatc atttttactg
    27061 catttaaaag cccttttgaa aaatctccat gagatgctaa tattatcttt ttcatacttc
    27121 atcctcctac taaatataga ttatatttct gctgatactg catatttgta cttatcactt
    27181 cttataaatt gctcattgta atataagaca tcattatttt ctgtatacac cgattgtctt
    27241 actttcaata gtggcatatt acttgcacat ttaagatagt cagaatattc tttgtttgaa
    27301 tgttttactt caaatatatt ttctactcgt ttaggtaatg tgcttatatt attagatata
    27361 acctccatta aagatttatc ttctaattct tcttttaata aaaacatgta atctattcta
    27421 aaatagtcaa cttcaaaaat aacaggtact tcatctatta atctaagtct tttaatacat
    27481 attaccttct cttctagttc aatacttaaa gcttcagcta cttgcttatc tgcttttttt
    27541 attaaaacag agattatttt ggtacttggt tttgcattaa tacgatgaca agaattagta
    27601 aaactatttc ctgcacatgt tgcttctatt ctctctggta ttgttacata agtcccttta
    27661 ccacgttttt tacataatat atcttctttt acaagttcat taagtgcatt tctcacagtt
    27721 atacgactta tattaaattc tttacataag tctccttcag atggaagttt atctcctggt
    27781 ttatatatgc cagatgctat ttgttttttt atgttgtcat atagttgttg atataaagga
    27841 acaaaggaat ttacttctaa atccatttct tttttttgtc tcttttcatc cattttgctc
    27901 acctccctat gtaacatttt tataacatta tataacaagt tctttattac atattataac
    27961 aagatgaaat cgttgtcaat ttttttttaa ataattttta atttgacagt attttctttt
    28021 attttctcta gtgtgacaaa ttgctatttt tacataaaaa taagactgtt gtacaacaga
    28081 ttaaattgtt tatacaacag cctttattca cttattacat ttatattttt acttataaat
    28141 ttatattaaa aaatgcctta tatcccttat acatttcata tatgtctact ttagagacaa
    28201 tctcatgtgg tgcatgcata tttaaaactc caacaccaca gtctataacc tctgcacctt
    28261 gatttgccaa tatatgggct atagttccgc ctcctccttg gtctactttt ccaagttcag
    28321 cagtttgcca tactacattt cctttattaa aaattctctt tacttctgac ataaattcag
    28381 cattagcatc attacatcca ctctttcctc ttgaacctgt atacttagtt aaaactactc
    28441 catttcccat atatgctgaa tttctctttt catatgcttc tccaaaattt gggtcatatc
    28501 cagcagaaac atcagctgat aataccttac tatttgctaa agctcttctt actttaatat
    28561 cacaataatc tccttctaaa gcaatcagtt cagccacagt attttcaaaa aactttgagt
    28621 gcatacctgt attcccttta gaaccaattt cttctttatc tacacataat gcaactgaag
    28681 taaattctgg ttgttcagtt tctagtatag cttttacagc agcatatgaa cataccctat
    28741 catcatgccc atatcccaac accatagacc tatcaaaccc taaatctcta gccttaccag
    28801 ctggaactac ttcaatttca gcacttaata agtcttcttc aactatattg tatttctcat
    28861 ttaaaatctt taatatattt gctgttatag gttctttttc ttcatcttct aaaggaatat
    28921 tcccaattaa tatatttaaa gcttctccac taattccttc tgatagtttc ttttgcattt
    28981 ggtctccagc caaatgaatc aataaatcag ttacacaaaa tactgggtca ctatcttctt
    29041 ccccaataga tatgtccact tttgttccat catttaatat aactatacca tgtaaagcaa
    29101 gtggaagagc tgtccactgg tattttttta ttccaccata ataatgagtc ttaaaaaaac
    29161 ctagattact ctcttgatac agtggatttg gttttaaatc taatcttggt gaatctatat
    29221 ggcttcctat aatcttcata cctttttcta tataattttt acctattaca aaaagtgcta
    29281 ctgccttctc tttatttata acataaatct tatcaccaca acttatgctt cctttttcca
    29341 tagcttcatc tattgaaata tagttatttt ctttagccaa ttttataatt tcttttactg
    29401 aaagtctttc agtctttgac ttacttaaaa actccatata attgcgagaa tattgcataa
    29461 tttgttcaat agtattttct tttctttcaa aatcccaagc attcttaaat tcgtacttta
    29521 agttcatatt ttcctcctac taagttctaa tttttattgt ttttcatgta ttgctcatat
    29581 tcttctggtg aaactacttt gacactatct agtcttgatc taaagtgagc tctaaagttt
    29641 tctaaatatt ctttccttaa aatttctctt tctttgattt catcagcaga aaggattccc
    29701 tctttatttt tctttgctaa ttcattaatc ctatctaatt tcttcttgtc aaacattttt
    29761 tcctcctaaa aaacctctat ttatttaata aaataattta ttgcttttta tatctaactt
    29821 gtttttatat tcacaaaaaa actcatctat attatcactt attacagata ttcccttttc
    29881 tagttcttcc atgcttgggc gagctatatt tatcctaaag aatctatcat ccactagatt
    29941 gtcaaaaaaa taagttcctg gaagcattga cactcctttg tttaacataa aatttgtaaa
    30001 tgcttgagat gtatatcctc ttggtaattc taaaaaaaag tttattcctc cacaagattt
    30061 tctaactttt aatttaccac tcaatttgtc attaatcaag ttttttgaca atttatactt
    30121 attagtatat atcttctcta tatgactcaa ataattcttc caatcaaaat tattcatata
    30181 gtaatacatt gatttttgaa ttaaacctgg tgttgatata tctgatgaat atttagccca
    30241 taaaactttt tttaacaact catttggcat ttctacaatt cctatcctaa gacctggcat
    30301 aagtattttt gaaaagcttt ttatatatac tactctattt ttatcatcat aacttctaat
    30361 tgttctattg tcctttgatt caaaaacaaa atcacttata aaatcatctt ctattatata
    30421 aaaatcatgt tcttctgcca attctatcaa tttctttttt ttatatgttg agtaagatat
    30481 tccagttgga ttttgaaaat taggcataat gtatataagt cttggtttta ttttttccaa
    30541 ctttaatttt aaaataccta tatcaattcc atcatctaac ataggtatag atattatttt
    30601 tgcacctcta cttttaaaca cttcaattgc tccattgtaa gaaggttctt ccataaaaac
    30661 aacatctgaa taacttataa gtcctttaca aactatatct ataccttgtt gagcacctga
    30721 tattatttga atattttcct tagtagtttt tatatttcca tcacttagat actctaccat
    30781 tttttctctt aattcttcag agccaagacc ttcatcatat tcaaatattg aactaccatc
    30841 tttagataat gccatattta cagcattttt aaatgaatcg ataggaaaca tatcattaga
    30901 tggattccca ttgtcaaaat gaattaaatc ttctctttgc acaataatct tatttttcct
    30961 gttatctata ccgcttttat aagaatttga acttgataca aaagtaccac tccctataat
    31021 tttatataca tatccttcat tttctaaaag ctcgtatacc ttaactatag tagtgttatt
    31081 tacttgtaaa aaatttgata gttttcttat tggaggcaat ttttcatgtt ctactaactc
    31141 tttatttatt atcaattctt ttatatgatt aaatatctgt atatatttag ttgatttttt
    31201 ctcatttaaa tctattctgt atttttccaa atttatcacc gctcattgta tttatatttt
    31261 attgatgctt ttatatatat ttgatattat ttagtatgta tatataatac caaaattttt
    31321 ataaaaaggg gaaatattta tgttttacga ctatcacatg cactcaagtt tctctacaga
    31381 cggaaaatct acaatggaag aaatggtaaa aaaatctatt gaactcggtt tggaggaaat
    31441 ttgtttcaca gaccatgttg actatgatgt atatgctgat gattcttttt caattgtcta
    31501 cgaagattat tttaagagtt tagagactct tcaaagcaaa tacaaagata aaatatcaat
    31561 aaaaaaagga attgaatttg gagttcaaac tcaattaatg gatacataca aaaaggaagc
    31621 acatcaatat ccattggact ttataatatg ttctatccat gctatagata caatggactt
    31681 ataccttggt aattatttta aagataaaac tcaatatgaa gtttatgaaa attactatct
    31741 ttatttatat aatattgtta agaattataa agattactct gtcttaggtc atttagattt
    31801 gattaaaagg tatgcacctt atgacactat tttagatgat agactatttt ctgatataat
    31861 tgaagaaact cttaaacaag ctatttatga tggcaaaggt atcgagataa atacctcttg
    31921 ttatagatat aatcttccag acctaacccc atctaagtat atacttcaaa tgtacaaaga
    31981 cttgggaggg gaaatcataa ccacaggttc agattcacat catataagtc aagttgcatg
    32041 tgagtttgac tacatatatt cattactaaa aaatatggga tttaaatatg tatctaaatt
    32101 taataaattg aaaccagaat ttataaaatt ataaattttt acacctcctt gtgaatatat
    32161 ttaaaagata aggaggtgat ttttttgaaa gtaccttatt tagtactatc aacagttatt
    32221 ttatttatat ccaattttat tgtaagaata tttggttttt tatataaaat attcttatca
    32281 agagcactag gtgaaacagg tttaggtatc tatcatatga tttttaactt tttgatgatt
    32341 tgcttagctg ttacaacaac aggtatacct actgcactta gttgtctagt tgcaaagaga
    32401 aaagctttaa acgatagaca caatactaat gctctattta tatcaacttt atatatatca
    32461 tttttcgtag cactaattat atctatagtt gcatctttca atagttcttt tttatcactt
    32521 aaatttttaa aagatgctaa attaaatcta tttatactag ctgtttgtcc tgctatagtt
    32581 ataataactc tttcaaacgt actaagagga tattactatg gcataaagaa tgtaaaaatc
    32641 cctgctattg gacaaatcat tgaacaaatt ggtaaaatat tatttgtttt tttacttgtt
    32701 atgtacataa ataataaatc tatgaattgt tatatagctc tattaggtat atctattggt
    32761 gagttgagca atattatatt tatgcttatt tgcttatggc gagattcatc atttgacaat
    32821 aggtatatta taagcataaa agatttttat aattcttcta tggaaacatt aaaaatgtct
    32881 attcccataa cttgtaatag aatgtcaaac atacttttgc aatctataag ttctatgatg
    32941 ataccatcaa gattagcatt atcaggaatg acttatcaac aatctttaag tatgtatggt
    33001 attgtcagtg ggatggtaat gccctttata tttttacctt ttactgttgg ttcagcgctt
    33061 attgtaaatt taataccaac tatttcacag gaaatggctc taagaaagcg taagagtgta
    33121 attaaaaaaa tcaaatactc catacttctt actttatttg taggcatact atcatcaata
    33181 tttttttatt tctttggaaa agatttatgt atattagtat ttaaaaatac acttgcagga
    33241 gaatatctaa aagctatgtt tttagttcca ctatttatgt ccctaaatca aactctatct
    33301 ggcattttac attcaataag aaaagaatta gcttcaagta taaatactat tactggcatg
    33361 ttaattcaac taatagcttt atatgtattt cttccaattc caggacttaa tatttatgca
    33421 tacatataca caatgacgat agtatcaatc tttacttgtc tattacatac tatagtgctt
    33481 tttaaatcac taaaatctat acgctagtta taaaaacgtg aaggctacca atgtaaatta
    33541 tacactttgg tagccttcct tagatgattt ttatttataa tttaaactgt ctatatatct
    33601 tacctatcta tctgtggcaa cttggctttc cacttccatg attaggtcca cattgcgaaa
    33661 aatcacatac tttagagaaa gtatcaacaa gtcttggttg gattggattt ggtgatgcta
    33721 aaactactat ttgttcactt attaatgtgc taactgtttc ttgtaagcaa agtactaaat
    33781 ctatatcatt tggtataatt aattcataga aaggttcaca acattcgttg cattctaatt
    33841 ttaatataga ttttcctata caatctacag ttaagttagt ttgatattct tgtcttaaag
    33901 taacagctct tccgtcagct ggcaagcata ttggtgctga aagtgtgtta aaagtaaatt
    33961 ctacataatc aactcttctc ttacatccac ctcctgctct aactgctggg aaagctaatg
    34021 caacgaactc tgtacatcca catctacctc ttagctctag tactaaatta cgaactgcta
    34081 cagttaatcc tctttgttta taagctactg atttgccttt tccttgtcta cagcactcag
    34141 cgtttctttc tccacaaact gcaaattcaa aagttgtttc acagtttctt cctactatat
    34201 ctcctaatgg gtctaaatcg aaatcttcta aagttgtcat tcctgtgtct ataactattc
    34261 catcattact taagcatatt ttttctatag ttacacttgc ttgacctgca cttggaattg
    34321 aaccaaatac ttctactact tctgtttcaa aagttaatgg ttctccattt ggacctgttg
    34381 catctgtaaa tgtttggaat tgcatagtat caaatattct attagcctct attgcaaatg
    34441 gcactgcatt tgatacagag aagtttcttg gagatatcgg attacaacat tctggtccac
    34501 atttgttttc aaaacattca tctgaatcca tttcacatgg ttcacaatca catttacact
    34561 tgtcatggca ttcacatttg tcatcacatg gattaggttt gcatggattg catgggttag
    34621 gtttacatgg attacatggg ttacatggat tacactcatc atggcactca cacttattat
    34681 cagtattaca tccattatga tggtgtgatt ttttaaaccc tctcatgtct tctgaattta
    34741 tttcatctgt atacacttct tcttcattca ttgtagacat tggctgattc atcattcttc
    34801 ttttattttt tttataatct tgcatttttc attgccctcc ctgtaagcga ttcttattaa
    34861 gtctttggct ttttgaagcc taattaatac tatgctttta taatcttaat tgttacttgc
    34921 tttttgaatt tatcaataat taggcgtatt tagtagaaaa atgcatatta ataaaaaaag
    34981 tactactatt tgaaatagta gcactttttt tgtcccgaaa caccggtggc taagtaaatt
    35041 cacaccctag gcactcccag gtgggtgact tgccagtaac ctttaacgtc caaactaact
    35101 aggcatgtta taatttacca gacacggtct cccgtatttc atttgtaggt tgaatttaaa
    35161 aaagccttat cgtatgtttc aggatttcat attattatta tataataaat tttgaatttt
    35221 ttaaagtggt tttatatagt aattgagata atcttattct atcattactt tttcttcctt
    35281 ttttctttat gtatttagtt aattatcgtt ttttcttagc tctagtttta gtattttttt
    35341 cattataatt gtattcggat tcttgctcat taatgttgta tagttcctca tcgtatacta
    35401 tatcttcatc attttctata ttttcatagc ctgtgttctt atcttctact aattcatctt
    35461 cattatcctc catttcacct tcatctgtta actcatcatc atccaattct ttgtcttcac
    35521 aatctaattc ttcgtcttca taatttaact catcattatt gtcttcatca tcattattac
    35581 tatcatcacc tcttaaaaga cttcctaaca atttgagatt attcatacta ccaagattgt
    35641 ttatatttgc caaagagctc aaactaaatc caccttcacc aaagtcatct ggtaaaaata
    35701 attctgcaaa taaatctatc ttctttttca ttcctatagc tttttcaact gaattttttc
    35761 ttttttctgg gaatacatct ataatttcct ttatcatcat attcttttta tcatcactat
    35821 ccatttttct gaaaaaatcc gtttcatctt ctgtatctac catatctata ttatttaatt
    35881 tttttattcc tctaactaaa tctaaaacac tttctatttt tattagtatt tttctttcat
    35941 ctcttgacat gtatttttta gttcttgtta ttatttccat tcccatttct aaatcttctt
    36001 cacttaaatc catgagcctt aaacctcttt ttatatcatt tgcatcaaac cttaaattgc
    36061 catgtgtaga ctgtgaaagt ttatcatttg catttttttt gttttttcta actctattat
    36121 ttccttttga tttattttta tcatcaaata tagagatatt accattactc aatgcaatca
    36181 ctccaatcaa aaatagtaag ttattcaaga caattccccc ttatattata taattgccct
    36241 atattaaagt atgtttttca tattttaatg ttccattttt aaagctcagt gcatatatat
    36301 tatctataag aatttttatt tatattatat atcactcaca attcaccact tcaatattat
    36361 ttctatatat aaatagtatg aatataattc aatatatatt tatagaaagg ggttttttta
    36421 tgaaaaaatt agatacagag tcattagaaa aaatggctaa acaaaaaaat attgacaagg
    36481 ataaaataga aaagatggct gatagttaca aaggaaaatc agaaaatgaa cttatggagg
    36541 aacttataaa aataggtaag aacttagatg gaagagatga agtagtatca aaattcaaag
    36601 catttttaga tgaaaatcaa cgcaaaaaac tagataatat aatggaaaaa atctcagatg
    36661 cagaaaatca aagagatact aaaccagcta aaactaagaa ggctaaacct actaaaggta
    36721 aatcaaattc acctgcacca aaaaatacac caggtggtca acaaaaaaaa tctaaaggtt
    36781 tatttaagaa aactaaaaag tctaaccctc atgagtagat aaaaataaac tatttaagta
    36841 attagataat tatttttagt taatctatta ttaaatttga tattattgac ataaaaaatc
    36901 aggtagtgcc ttaggcacta cctgattgat tttaatagtt ttattttttt ctttctgtaa
    36961 cactttctct atcaacaatt ctataaggta attctatttt cttattttca attaattcct
    37021 tatttattaa ttttataacc attcttattg ctactgctcc catatcgtat agaggttgat
    37081 atactgtagt aagtttaggt ctatacattc tagctatctt aacatcatta aatcctgcaa
    37141 cagatatgtc ttctggaacc ttatatccag catcacatat agcatttata gcacctatgg
    37201 cagcctcatc acctgttaca aaagccgcat gaggtattat tccatcatct aatagttctt
    37261 tcatactatt gtatccactc tcataatcag ttccaccata cttaatcaaa ctcttgtcta
    37321 attctatatt gttatttgaa agtgctttct cataaccagc aagtctttcc atttctaaaa
    37381 cagtatcatc tttactcgtc attataaaag ctatcttttc atgacctttc tttataagat
    37441 gctctgtcat atcaaaagta gcttcttcgt tgctagtact tactgtatat atatcataat
    37501 ttctagcagt tttacttata tatgtcgctg gtattccaca attttgtata tagttgatat
    37561 gttcttgttc aactatccat gaaatcataa ctataccttc cacttgtttt gctctcaata
    37621 gatttatact cttaagttct tgttccttat cagagtatgt attcgctaaa agaatatcat
    37681 agtcatacat tttagcaact tcttctatcc catttaacac ctcattaaca aaagaatctg
    37741 aaacttctgg aactattacc cctattaatt gactcttctt tgttactaag cttctagcaa
    37801 gtggatttgg tatatatcca gtctctttta taacctctaa aactttttgt ttgacttcat
    37861 cagttacagg ttttgaatca tttataactc tagatacagt agatattgac actcctgctt
    37921 gtttagcaac atcttttatc gttatattgc ctttcatctt catcctcctt attttactaa
    37981 atttatctaa aactgaacca aatttttgaa ggttcctatt ttaactacat ttatataagt
    38041 ttttatttta ccataaaagc aagtacattt aaatacatta tattttatat tataccctat
    38101 attcaacaaa tttaataata ttcattctta atttaaatca tattaaaaaa taaaataaaa
    38161 aaatactggt ataaaatacc agcatttttt tattctaatg atttaagtat tagcagccgc
    38221 aacctacttc accatcaaag aatccaccac atgaacaagt tattagcaat aatattaaga
    38281 aaggtatcca tgacattatg tcaatgtctc tccagcaatc ttcaaaagct aagaatagga
    38341 agaatagtac tattatccac catcctcctc caccaaagca tccttcttct ccaaaaaatc
    38401 tatctaacat aacaaagccc tccttttttt aataactgtg ttcttattct aagcatccac
    38461 cacagcagca aagtattaat aaaactatta ggaatggtat ccatgccatt atgtcaaatt
    38521 ctgcccagct atctccaaaa gctaagaata ggaagaatag tactattatc caccatcctc
    38581 ctccaccaaa acatccttct tctccaaaga atctatctaa cattgtacgt tacctcctgt
    38641 tgatattttg ttgagctttt cgctcctaac atatactatg catttggttt ccattttgct
    38701 aatgtatttt tactattttt ttaaattttt taatttgaac taaaaaaggt aaaaccataa
    38761 cttgtataag ttactggttt tacctattta ataatcaata ttttttagca acaacaacca
    38821 tcatcgaagt taaataataa caatactatt aatataaccc atatccatat attgtcatcg
    38881 cataataagt cttctaaaat gtctgtgcat aatagtaaga atactataac tattattatc
    38941 aacattttat taccaaaaaa tccttcgcat cctccaaaga aacgttccat attgtcttct
    39001 aattttgtca taactaatcc tccttgaatc attaagagac taagttttgt ctctttaatt
    39061 aaatatatgt agtaatagac agataagtta cttctaaaaa gtttttattg attagtttat
    39121 ttatttcttt ttcaccaagt ttattgtatc tatcaacttc atccagataa tcttttttct
    39181 cctctgatat agtgtagatt aaactttcta ttgattttat tttgtactcc ttatatcttt
    39241 ctatcaagaa ggatgttttt gaattttcta ttcctttctt tacatgcatt aaccttgata
    39301 tgtagtcttc tttttcatta gatacataac tctccttttc agctacatct tcaaggatta
    39361 aattggtctc cattacataa tcctctatac gttgcatatc taaagttatt gaattataat
    39421 aaaaatttac tgctataagt aaaaacattc ctactgaagt tagtaatagt cctacatatt
    39481 tcatagcatt tcctcctaag ttatatttgt tatcaataaa gtttattcat caaataatac
    39541 tttattacta catttataaa attttattat tttttataga ttccatattg aaaaattctt
    39601 gatattatat taaactaaca ttaaaattaa ttaaaactgc tttaggaggc ttaaaatgtt
    39661 tgagaaaata aaagaaataa tagcagaaca attaggggta gacaatttag atgagataac
    39721 tatggaagca tctttaatgg acgacttaga agctgattca ttagatgctg ttgaaattat
    39781 aatggccctt gaagatgaat ttggaataga aataccagac gaggaagctg agaactttaa
    39841 atgtattggt gatatatgta aatatatcga agaaaacaaa tagtatcgtt ttgtctacaa
    39901 atataaatag tctgtgtaaa cttctacata ataaattata ttagagttcg cacagactag
    39961 attacattta tttacaatat ataaaataca cgctcttata tttaatttat atactaagta
    40021 tataaattac ttttcttcat aaaatgtaac attctttatt attatatcta tagttccatc
    40081 ttgattttgc tttacagtat acttcattgg gtcttcaaaa tcagttagat tcccttttat
    40141 gtcaaaacca ttatccgttt ttatacttct ttttttaagc tttttctcaa cccatttttt
    40201 atctatacta aaactttcat caagaccttt ttcttccata tgctctttaa agctatcctt
    40261 taactcatca tctttaattg aattatcaac aaagtcattt atatcaactt catgtttttc
    40321 cttcagtgta taatttaata tacttcttac atcttcagct tgctttatat cattactaag
    40381 agcattagtt atccaatttt cagctgtatt tttaaatacc ttggtcttgt acttatcatc
    40441 atctattttt ttagcattta aaaattctgt tacaaactta gattctgacc cttctttttc
    40501 agcatcctta tctaaaagcc ttagatgaaa ctcatcattt ataccactta aactaatcaa
    40561 agctccttgc ttttgtctta aagtctctgg gataccaatt tcatttgaaa ccatttgaat
    40621 attaaattta tcatccacaa attcaattga atgagtgtac aactttttat aatctaattt
    40681 cagtattgct acactctttt catctttaat agtgtataag caaattgcca aatcacaaga
    40741 ttctagtgca gcattaattt tcataacgtc aaacaagtaa gatgcaattt ctttagaatt
    40801 tttcaaaaaa gtactttcat catatattat ttgttcacaa cagtttttaa ttaaattgtc
    40861 attataatct ttaaaaacac cttttcttaa atcctcatct tttgcaattc tttttatagc
    40921 tttctggaaa aaaccatcta cttcttgatt aactttgcct tcaaagtcat ttaatattgg
    40981 aacatcacta tttttgtcca aaacatgtat tataaattta tgtattatca tatatatcac
    41041 cccattatct taatttcgat attaactctt tcatatcctc tggtatatct gctttaaatt
    41101 tcaatttttc ttttgttctt ggttgttcaa actctagctt gtatgcatga agtgcttgcc
    41161 tatttattag ctcttcatct acataaccat acaactcatc acctattatt ccatgaccta
    41221 tatgagccat atgtactctt atttgatgtg ttctaccagt ttctaagctt acttctaata
    41281 cagttgcatt ttctagtctt tctattactt tatagtgagt aacagatgac tgtcctcttt
    41341 catctattat cctctttatt gaatcttctg taggtctata aattggctca tctatagttc
    41401 cctcattttc ttttataatt ccatcaacta ctgttatata ctttttttca actttatttt
    41461 cactcattgc tgtagataaa gtatgatgtg cataagcatt ttttgctact ataactaacc
    41521 ctgaagtatt catatctaac ctatttacaa atcttatttt tacgttttct ttttgattat
    41581 ctatatagta actaatacca tttgctattg ttttatcata gtggcttttt gttgggtgaa
    41641 ctaccataaa tggaggttta ttcaccataa ttatatcaaa atcatcataa attatctgta
    41701 aatttaaatc ttgaggttca aaatttgctt tttcctcgtc tatttttact tctattaaat
    41761 caccactata cacctttaaa cttggttttt tatatactcc atttactaaa acactctttt
    41821 ccttcttcat ttttgataat gacctaactg agaaatttag cttgtccaat aaaacttctt
    41881 ttaaagtcat ttcttcttca tttgtatacg atataagatt atatctttga ttctcttttt
    41941 taaacaaact tttacctact ttcttttata tcttcctgct ttttatatct tcaaaatcat
    42001 gatattatat attttacact atttttgttt aatagataat aattattttt aactataaaa
    42061 acctgtaaga ataacaaata gttattcata caggttaaat tcataattaa atatttaatt
    42121 tgtaaatatt ataatctaaa actatctctt aactattaaa gtagttccca ttccaccacc
    42181 tatacaaagt gtagcaagac cagttttagc atctcttctc ttcatttcat ataaaagtgt
    42241 agtaagtatt cttgctcctg agcatcctat tggatgtcct atagctattg ctccaccatt
    42301 aacattaact ttattcatat ctatatttaa gtctcttatt acagctacag attgggcagc
    42361 aaatgcctca ttagcttcaa ctaaatctat atcttcaata gtcatattag cagcttctaa
    42421 agctttttta gttgctggaa ctggtccata tcccattatt ttagggtcaa caccagctgt
    42481 tccataagaa actatagttg caagaggctc tattcctagt tcttcagctt tttctttagc
    42541 cattactact agcatagcag caccatcatt tattcctgat gcattaccag cagtaactgt
    42601 tccatctttt ttaaatgcag gtcttaactt agcaagtttt tccattgtag tgccaggctt
    42661 aatatattca tctttatcta ctacagtgtc accttttctt ccttttataa caacaggaac
    42721 tatttcttca tcaaattttc cttcagcttg agctttttca gctttatttt gacttgcaag
    42781 agctaattca tcttgttctt ctctagttat attccattgc tctgctatgt tttcagcagt
    42841 aatacccatg tgatagttat taaatatgtc tgataatcca tcttttatca ttgaatcaac
    42901 aaaagcagca tcacccattc ttgcaccata tctcgcactt ggtactaaat aaggagacat
    42961 actcatattt tcagctccac caactaacat tatatcagca tcacctaatg ctataagttg
    43021 agatgccatt gaaacagatc ttaatccaga accacaaact atatttatag tcatagctgg
    43081 tttttctact ggtattcctg ctcctaatgc tatttgtctt gctatatttt gtccaagacc
    43141 tgctgtaagt actcccccta aaagagattc atctatcata tctggagtta tgttagctct
    43201 ttttatagct tctttagctg ctgttacccc taactctacc gctgaaactg atttaaatgc
    43261 tcctccaaaa cttcctactg ctgttctagc tgcactggca attactactt ctctcatatt
    43321 cattaccccc attaaattag atttttcata tatccgaaat tgttttttaa acaattttat
    43381 ttaaactttt aattcccaaa ttcttattta ttataatcat agaatcctat cttagttttt
    43441 cttcctaatt gattagctct aaccatttta gctaaaagtg gatgaggtct atatttagta
    43501 tctccaaatt cagtatataa aacgttcatt atagctaaaa caacatctaa tccgattaaa
    43561 tcacctaatg ctagtggtcc cattggatgg tttgctccta atttcatagc ttcatctatt
    43621 tcttctttac ttgcaacacc atctgcatat ataccaacag cttcatttat cataggtata
    43681 agtattctat ttactacaaa tccaggagat tcagatacat ctactggtac tttattgata
    43741 ctcttagata attcaaatac tgtatcaaaa gtaacttttg atgttaactg accacttata
    43801 acttcaacta atttcatcat aggaactgga ttaaagaaat gcattcctat aactttatct
    43861 ggtctcttag tagaagaagc tatttctgtt atagataatg atgaagtatt tgttgccaag
    43921 atagtatctt ctttacataa ttcatctagt aacttgaaaa catctttctt tatattcatg
    43981 tcttctacag atgcttctat tattaaatcc atatctttta aatcttcata attagtagtt
    44041 gaactaacat gacttaatat ttctgctttt gtagcttcat ccatttttcc cttagtaact
    44101 aacttagtta aatttttatc taataaagct aaacatttat ctatagcacc ttgagttcta
    44161 ctctttaaac atacatcatg tccacaactt gcaaaagttt gtacaatacc acttcccata
    44221 gttccactac ctattacagc taatttcata gttataacct cctgttattt tagtatttat
    44281 attctgttaa ctagattaaa atactattta atctagttaa cagcaaatat atttttaatt
    44341 agatttttac ccttttataa agttagcttc tctcttttca acgaaagctg acattccttc
    44401 tttttggtct ttagttgaaa aacaaagacc aaataaatta gattctatat ctattccagt
    44461 atttatatca gtttgagcac caagttgtat tgcttcttta gagtatctaa ctgcaagctg
    44521 agcattttta gctattatct tagctaattt ctcaacttct tctattaaaa tgtctggctc
    44581 aacgactcta tttactagcc ctattttttc agcttcatca gcttttataa cttgacctgt
    44641 aaagattaat tcttttgctt ttgccattcc aaccaatctt gtaagtcttt gagttcctcc
    44701 atatcctgga gttattccaa gagttacttc tggctgacca aatttagctt tagcagatgc
    44761 aattcttata tcacatgcca ttgcaagttc acatcctcca cctaaagcaa atccgtttac
    44821 agcagctatc actacttttt tactattttc tatttctcca aaagcttttg ctcctaagat
    44881 actaaaatct ttagcagcta cagcatctaa atctttcatg tatgcaatat ctgctccagc
    44941 tacaaatgcc tttccttccc ctgtcaatat tacaacatca atagtttcat cattattaat
    45001 atctacaaat acttcataaa gttcttctaa agtctttgaa tttattgcat taagggcttt
    45061 aggtctattc attttcactg tacatatatt tccatctact tcaacagcta cattctcata
    45121 aactttaaca tcacttgtac tcataatacc ctccccaaga aattagttag ctaaaacttc
    45181 acctttttct tttgcaacac ttaactgact gataagttct ggaagcactt tatgaacatc
    45241 tccaactata ccaacatcag catatttaaa tattggagct tctggatttt tatttatagc
    45301 aactataaac tcagcatctt ccataccagc tatatgttgt attgctccag atataccaca
    45361 tgctatataa aggtctggtc ttacagtttt accagtttga ccaacttgtc ttgctttatc
    45421 taaccaacct gcatctatag tggcacgaga accagaaact tctccaccta taatttcagc
    45481 taattcataa agtatgtcta agttttcttt tccacccatt ccacgtccag cagaaactaa
    45541 tatcttagca tcttctattt taacttgttt tttagcttct tttattactt gtacaacttg
    45601 aactaattta tcagcatcat taaattctac cttgaaacgg ttaattacag cttctttagt
    45661 ttcatcaggt tcatttttct tcataacccc tggtctaact gtagacattt gaggtctgaa
    45721 atctttacaa actattgttg ccattatatt tccaccaaag gcaggtcttg tcattaataa
    45781 taattttgta tcttcagcta ctgcaagacc tgtacagtca gcagtaagac ctgtatgtat
    45841 tctagcagaa actctaggcg ctaaatctct acctattgaa gttgcaccaa ataatacaac
    45901 tatagggtca gctgctttta ttgcttcata agctgctttt gtatatggtt cagttgtata
    45961 cactgctaaa gcttcatcat ctactactat tacctcatct gcaccatagt gtgctaatgt
    46021 atctattaaa ccttctacct tactacctaa aagtaatgca gaaacttttg tatcataatc
    46081 ttttgctatt tctgtagcct ttcctagtaa ttctaaagaa acagtttgaa ttacattttc
    46141 tctttgttct attactacta aaacgttacc cattctttat ccccccttaa attatatgat
    46201 atacttctct tttaatttat ctatgataat tccagctgat gttttcgcat cttcattgta
    46261 tattgtacca gctggtttaa ctgattttgt aaatgattta aatacactag ttggagaacc
    46321 tttaagacct aaattagaag ggtcaacttc tatatctttt acagtccatg tttctactac
    46381 atcattttcg aaagcatcat atattcttcc aactttcatg tatcttggtg tgttcatatc
    46441 tttaagagtt gttataaggc atggcatttt aacttttaag tcatggcaac aatcttcaaa
    46501 ttgtcttttt actaatacat attcaccttc agtttttatt tcttcagcat atgttattga
    46561 tggaagattt aaatgttcag ctatttgagg tccaacttgt gcagtatctc catctatcgc
    46621 ctgtcttcca gctattataa tatcaaaatc tatatttttt aatgctcctg ctaatgctga
    46681 tgaagttgcc caagtatcag cacctgcaaa tgctctatct gttaataata tacctctatc
    46741 tgcacccatt gctaaagctt cttttaaagc catatctgct tgaggaggtc ccattgttat
    46801 aacagttaca tgagcaccca tttcttcttt taattttata gcttcttcta aacctgcttt
    46861 atcatcaggg tttattatac ttggtactcc atctctaatt aaagtacctg tattaggatc
    46921 tagtttaact tctgttgtat ctggaacttg ttttatacaa acgactatat tcataaatcc
    46981 ttcctccata tctattttaa tatcgaccta ttgtgtatat tttaatattt atctatttta
    47041 ataattttcc tgaaataacc attctttgaa cttcactagt tccttcatat atttcagtta
    47101 tcttagcatc tctcatcatt ctttctactg gatagtcacg agtgtatcca tatcctccat
    47161 gaagttgtac agctttcgta gtaacttcca tagctgtttc agctgcaaat aattttgcca
    47221 ttgctgcttc tactccataa ggttttccta agtctttatt tatagctgct tgatatacaa
    47281 ggtgtctagc cgcttgtact ttaacttcca tatcagctaa ttggaattgg gtattttgga
    47341 attttgataa tggtctacca aattgtactc tttcttttac atatttaaca gtttcatcaa
    47401 gagcaccttg tgctaaacct aaagcttgtg ctgctatacc aattctacca ccatcaagag
    47461 tagacattgc tatcttaaat ccttgacctt cttttccaag taaattttct ttaggtattc
    47521 tgcaatcctc aaatattaat tcactagtag ctgaacctct tatacccatt ttcttttctt
    47581 taactccaaa gctaaaccca ggagttcctt tttcaactat aaatgctgat attcctttgt
    47641 tccccttaga tttatcagtc attgccatta ctacatatat gtcaccagct attgcatttg
    47701 ttataaatat ttttgagcca ttaagtatgt attcatcccc gtctaaaaca gcagttgttt
    47761 gttggccaga cgcatctgta ccagcattag gctcagtaag accaaatgct cctaatttat
    47821 ctccacttgc tagtggtttt aagaattttt gtttttgttc ttcattacca tattgatata
    47881 taggccatga gccaagagat gtatgagctg ataatataac tcctgtagta ccacaaactc
    47941 tagacaattc ttcaactgcc attatatatc ctacagtgtc tccaccttct ccaccatatt
    48001 cttttggata tggtataccc atcattcctg cttttgccat tttttccact gtttcataag
    48061 gaaatctttc ttcttcatca agttctgttg ctaaaggttt aacttcattt tcagcgaagc
    48121 ttacatatag ctctttaagc atctgatatt ttttagaatt taaatccata atagcctcct
    48181 cagtatttta taaatatttt attttcattt attaattaaa atctcggcaa catctataat
    48241 aaggtatttg aatatacgtc gagaaattta taaccttaat caaactacgc tctatattta
    48301 tatgtgatta tactaaccaa ttatgacatt aatttaatca taaattgtta atttaaaatc
    48361 aactatactt aaaaaaaatc tcgctttttg gaattttgtg aaatttcaag ttagaataag
    48421 atttctttca cactggtcac agataaaata atttgtttac gatatttata ttagggtata
    48481 ttaaacatta aatattgtct gtacatctgt tttttagcca tgtacagaag atttttatcg
    48541 tataaaaatt attttaatag agctaaataa tcgtattttt atctgactat tttttatttt
    48601 taaatatctt acatctataa tagtaacact caaaggataa tttttcaata atttctaatt
    48661 atttttttaa caatatttat tacatttttt ccactttttt gtcatttttt tattgtttcg
    48721 aaaaaaaatc agaatttttt aactttattt tggattatta gtttaaatta gattttcctc
    48781 ttagatttat aaatatttac acaaaaaact acctctgtat agattgtata cagaggtagt
    48841 tttttactaa ttcctactat agtttactat atttttttac caattcttac aaacatctac
    48901 ccatttttca ctcttagaaa acttctccat ttcttcacat gtaagttcta ttgcagagtt
    48961 actactgcca catgcaggaa atactgtatc aaatctcttc attgaatcat ctaaatagac
    49021 ttttatagaa ttttttaaac caaatggaca gactccacca atagcatgtc cagtaaactc
    49081 caagacttct tctggtgtca acattttagc cttacaatta aattcagcct tatacttctt
    49141 attatcaatc ttagcatcac cagctgtcac aattaaaatt gcatcatctc ctattttaaa
    49201 tgagagtgtc ttagctattc ttgctggaat aaccccagct gcttcagctg caagttccac
    49261 agtagcacta gattgttcaa attctaatat actgtcttcc ttgccaaatt gtttaaaata
    49321 ctctcttaca ttacttattg acattaaata cccctcctag tctgaatttt tataaatttc
    49381 atattattct taattatatc tataattgat ttatttttca aattattcca taatattagc
    49441 attccatgta tcattatttt ttccaactac tagataaagt ccttttgcca ttggaatatt
    49501 gacaatatat tctcctttat attctacact cataccagat tcattaaagt acctataatc
    49561 aaaatttagt ccttttggaa ttacaaaatc aacataacct tgattatttg gttttattcc
    49621 atatttcaga gtactttcag ttatagaatt tttttcatta aataaatcta catttacata
    49681 tgcagaacac ttattcattc cctttttatt ttccacacaa ttttcaaact tcattttagc
    49741 actgtttaat ttataactat atttactatt attgcttatt ttaaatctta caatagtacc
    49801 gatatctcta cttctcctgc tatcaaaatc aaaaacaatt tcctctaaat gatttatttc
    49861 ataatctttg acacttacca tatctctata tactaattta tttatagcta tgaatagtgt
    49921 aaaaatcccc aaaataaaaa atgaaacttt tataaacttc ttcattttta gtctccttat
    49981 attttgtgct attgtgctat aatattaatt aaaattttaa taattaaact tattcttttt
    50041 tgaaatctta atagaaaaat acattgatat tataaaaatt attattgtta aaaatgtcat
    50101 actatataaa tttatatact tagtattagc tatatcagtt acataatttg aaagatatac
    50161 tcctccaact ttactataat taaaatgctt tacttgatat acaatcatac taatcatctt
    50221 caaaagactt ccatatacaa agtaaattaa aatacttact ataaaaccac aaactttcat
    50281 ttttccatat ccaaacttaa aaattaaagg cagtaaaatc gtcaaaattg ctaaaaatat
    50341 atttacagaa aaaaatacat catttaaaac catatttcta aagaacacta gtgatgtcaa
    50401 tttacttagt aaggacacaa ttattgttac agaaattatt agtcctacac ctaataaata
    50461 ttttgagtaa actatatctt ctatactaac tggcatactc cttataaaaa tccttgaatc
    50521 attttcataa tcacactcaa atgtatttat aaatatcaaa taactaacaa caatacttac
    50581 tgtataatat gaaattgaat taaggaaaaa ataaaacaca aaaaatagca atatgtactt
    50641 taaattacgg ataccatctg aactaattcc tattattaaa tcttttctta ttaaattgaa
    50701 catcctctca cctcatttac ataataatac attagttctt ctatagtagg atttttgacc
    50761 ttatgcttat caatttctat caataggctt tttactttca agctatctcc aactttaaca
    50821 agtgcctcat tataatattt atactcttta acaccaacta tactttcttt aattgcttct
    50881 aactctgctt tatttgccct aattatctta tattcttgtt ctattaattc tttcttaaca
    50941 gtaaaaatct gatttcctct atttataaaa acaagataat cagcaatttt aattaaatta
    51001 tcagtattat gagtagaaat tattacacta ctacttccat ttttcacaaa atcttgtaag
    51061 tactgcatta tttcagttct tacaatcggg tctataccag cagttggttc atccaaaatc
    51121 aataacttag cattatgact aagtgcgctt gataacataa gcttctcaga ttgaccttta
    51181 gataaatttt caatatgcat attttcttct atgtcaaact tattaaggta ttcatcaaat
    51241 ttttctctat caaaattctt ataaaataaa gaaactattt ttctaaattc cttaaccttt
    51301 aaatgtggat aaaattgtaa agaatcatat acaaaaccaa tattgttttt aaagcttatt
    51361 ggagaatttt caatagtttc attacaaagt tcaatttcac cttcatctgc atcaataatc
    51421 cccattataa tttttattaa agtcgttttt cccgaaccat ttggtcctat aatcccaaca
    51481 atatcccctt cctttaactc taagtttata ttatttaact tgaattttcc taattttttt
    51541 gtgatattcc ttatgctaaa cacatcttca cctcttatgc atttttttat acccacctat
    51601 acatatttta acacattcac tacaaaggtc atttataaat ttataaatta agaattataa
    51661 aactatgtta taatacaaag tatgaagagg tgaatatatg aaaagaatta taactataaa
    51721 tacaaaccaa ttaaataagt cccttgagac taaagaactc ttaactagaa aacttatcaa
    51781 tgctggcttt gaagtgtatt cagatattta tccagacaca gagcttatta tatcaattgg
    51841 tggtgatggc tcttttttaa gaacagttag agatttcgat tttcctgaga ttcccataat
    51901 gggaataaat acaggtcatc tggggttttt tcctgatatt ttaccagata agattgatag
    51961 ctttattgaa gcttatacaa aaaaagatta tattattcaa gaaatgtcac ttcttaatgc
    52021 ggaagtatat acaactacta gtggttctaa tatgcttgct gtaaatgagg ttgttataag
    52081 aggagacaag tctaggacta tacacctaaa tttaagcctt gataataaac atatacaaaa
    52141 ttttagtgga gatggtatga ttatttctac ttccacaggt tcaacagcct ataactattc
    52201 tgctggaggt agtattgttg atataaatct tgaacttatg cagattacac ctttacatcc
    52261 gataaatacc aatgcatata ggtgctttac atcaagtata atatgttcaa atgaatctgt
    52321 tataaagatt gcaccagaat atagatttga agattctgta ttgattgtcg ttgatggagt
    52381 tgagcacaga tttagacaaa tcgaaaatat aaaagttagt atatctgatg caaaaataaa
    52441 attacttaga atgtcaaact atgagttttg gcatagagta tctgaaaaat ttttatagac
    52501 tgtacttagg aggattttta atatgaaaat aggaataatg agtgatactc atggtagctt
    52561 actttacttt gaaaaagcgt taaatgtatt atctgattgt gatgttttat tacatggtgg
    52621 agatgtctta tatcatggac ctagaaatga tattccagaa ggatataatc ctaaaaaatt
    52681 tattgagaca ttaaataaat tagaaaacat tgtgattgta aaagggaact gtgatgcaga
    52741 tgttgaccag atggttatag aacatcctat tcagagtcct tatgtcatga gccaatttgg
    52801 tgaaattaga attatattga atcatggtta tatagaatca gaagaagaaa ttattgacaa
    52861 agcaaagaaa atgggtggtg acatattagt tttaggacat actcatgtaa aaaaactata
    52921 catggatgat aatttaatag taataaatcc aggaagtact tctatcccaa aagatggaag
    52981 ccattctgtc gcaattatag atatcataaa aactgatgat gaggatgagc ttgaacttga
    53041 tataaactta attgacatca atactggaaa tataataaat atcaatgatt agtaaatttt
    53101 taataaaaat aaaataagct ttaatttatt aaactcataa taaaaacaag taaaccattt
    53161 tatttctatc tcaattagaa gtttaagtac aataccttaa acttctaatt aaatagaatc
    53221 tggtttactt gttttattat atgctatatt gaaatataac ttagtttact tattttatta
    53281 tatatattga agttcaactt ggtttacttg ctattattat atattaattt tgatttacac
    53341 aatattactg taaggctaat gcttctgcat atggtttact atttttacct ccaccaactt
    53401 gtacaaattt atcagccttt acttttttta taaaactttt ctgtgcacta cttaaacttc
    53461 cacttgcaag tactacaggt gactttgttt ttgatgctaa tactccagca gcaagcccat
    53521 caactaaatc agcttctcta ccacttccat cttttgcaaa atacatatta ttaaccttac
    53581 ttgattttcc ataaaacttt tctaaaactt tcgcattagt atcttttcta tctgttcctg
    53641 ctagtctttc tgagtttact aactgtcctt caagtttttt acttatactg tactccccac
    53701 ctactatgta tgtcttagtt gagttttgtg taataaaatt ctttgcctca cttcttatgc
    53761 tatccttacc tgtatatatt atagctgtat tatcacttgc agctgggctt gatattgaag
    53821 ttgcatctgc cagtccatta tacccattaa ccactgccac ttttgagaca cctaaatcac
    53881 cagtatcatc tagctgtcta gtaagaagta aagatgtttc ttctctatct gctccaccga
    53941 ttctactgac taatacttcc atatcttcaa gagattcttt tacacttgat gaaatagctc
    54001 cttcacttcc aattaaaata acttctgctg gattaagcct ttttatttcc tctgttacat
    54061 tatatggtat actattttta tttgtaagca gtattggtgc atccaattta cttgccaaag
    54121 gagttgaagc caatgcatct ggcaatgaat ttgagttaac tagaaacacc ctttctgaac
    54181 cattattcca accactttga gatattttta tcgctgtatc aaatctatct tcaccaataa
    54241 gtttttcctc ctttggagat tttctacctt gaagataatt gttcatatct acctgtgcat
    54301 tttgattttt actaatagtg tctgcatata cacaacttgg caacataatc ccaagtgaaa
    54361 gtgcaaatgc tattattttt ttagatttaa ttttcaatac aattccccct tttaaatgtt
    54421 aatacctatt gatatccata ctttttattg ataccctaaa ctaaaaataa ttatctttta
    54481 taaaaataat tacaaaaata gctataccat tttaaataca acacaagctt ataatcagct
    54541 tgacttataa agaatgatat agctattata actttaaatg cttaattttt aaatactttt
    54601 taactaaact ctttcagcat atttttcagc tattcttaca attagtttag tagctttctc
    54661 catagaacta actggtatac actcattttt cccatggaag tttaatccac cagtaaatat
    54721 atttggacaa ggtaatccat taaaagataa tcttgctcca tcagtaccac ctcttacagg
    54781 aactaaacgt ggttttatac ctaattcctc catagcttct ttagctatat caactataaa
    54841 cataactggc tcaatctttt ctttcatatt gtaataagaa tcttttaaat ccaattccac
    54901 tctattatcg tatttcttat ttattttttc tatattctct atcataatag attttctatt
    54961 ttcaaatttt tctctacaat ggtctctaat aatataagcc atacttacat tctcgacatt
    55021 tccacctata gaatttaaat gccaaaatcc ttcatatcct tctgttgttt ctggtctttc
    55081 atctgctggg aacatttctg atatttcagc agctatatgt atagcattta ccattttatt
    55141 tttagctgaa cctggatgaa catttctacc atgtatagta atagttgcat ttgcagcatt
    55201 aaaattttca cattcaagtt ctccaactat tcctccatct aaagtatatg catattttgc
    55261 tccaaatttt tcaacatcaa ataaatcagc acctctacca atttcttcat ctggtgtaaa
    55321 tcctattttt atatctccat gcttaatttc tggatgattt attagatact ctattgcagt
    55381 cactatctca gctataccag ctttatcatc agagccaagt agagttgttc catcagtgac
    55441 tattaaatcg tcacctttaa attcttccaa ctctggagaa tcctttacat acgtaataac
    55501 atttaacgct tcatttaaaa ctatgtcttt tccgtcataa ttttctatta ttcttggttt
    55561 aatatctttt ccactaacat cttcaattgt atctaaatgt gatatgaatc ctatagtatc
    55621 cacaccatca gtatttcctt ttaaagtagc cattatataa ctattttcat ctaaactcac
    55681 atcatcaaca cccattgttt ttagctcttc aactatatac ttagctaaaa ctctttgacc
    55741 ttctgaagat ggacagttct cacactgaga attagatgtt gtatcaaatg atacatattt
    55801 taaaaatctt tccactactt tttgtttcac gtatataccc tctttcttaa taagattaat
    55861 atttttttat ttacctgtga ataaaatttt atttttaaaa aaatctatca attattataa
    55921 ttaaaaaata tccaccttct taagtatatc atgcatacat accaaaccca aacaaaaaat
    55981 tactccaatt attcctttta cttggattcc aataaaaatc gctgctaggg tagctaatgg
    56041 atttagtcca acatttactg atactagttt aggttcaagt atttctctaa tcaaagacaa
    56101 aacaaaaaat atcacagtca tacttatagc tataaaataa ttttttataa gaaaataata
    56161 aacaattact ggaagatata ttgttattat acctaggaat ggaattaaat ccattaaagc
    56221 cctacaaacc ctactattag accgtatttc aatccaaata aagcaaaact tccccagatt
    56281 gcaaaaaatg ttatagccat aagtattgta taggccctta tatatccaac aagtgaaagc
    56341 cctgcctctt tctttacgtt ttttaccttc tttctaacat caacagtaaa catattataa
    56401 aaactatttt caatcttatc aaggtctttt gctataaagt aggtagcaat aaacaaggta
    56461 attataaata ttataatata tggaatagaa gtagccaatc caagtatact agtaagaaga
    56521 ttttttgtaa tttgcattac ttcaccactg aatttgttta ctaattgttc tacatcaaaa
    56581 ttggctattt cttgaaatga tttcataaag tcatttattt tatttaaaaa atacataata
    56641 gtattactta tatcctcagg attagcagat atattattta aaaatcccat taattctttc
    56701 attgaattta ttactattat tgtagtaact gtactgacta tagccactgc aagaaaactt
    56761 agaacaagag tagagatacc tttatttata cgaaacttat tttctaactt ttgtgatata
    56821 ggattaatca taagtgctat tatagcacct agaaaaaatg gagctatgta tggaaacgct
    56881 ttgtaaataa aaataaataa caatgtatat atacaaaaaa agatagtgtt gttttttaat
    56941 ttgtataaaa aattattttc tagtatgctc atcttaaaaa tctcctttct tctaatttat
    57001 atttttattt tgtccatttc atactagttt tatttgaata caaaataaaa agaagtgtat
    57061 aa
//
LOCUS       DAMJVX010000009        55911 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMJVX010000009 DAMJVX010000000
VERSION     DAMJVX010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55911
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.009"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            75..917
                     /locus_tag="PC407_000482"
     CDS             75..917
                     /locus_tag="PC407_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2495962.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            932..1396
                     /locus_tag="PC407_000483"
     CDS             932..1396
                     /locus_tag="PC407_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495963.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIITCFGQMIALKTQAQDLLIGSISG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            1383..2555
                     /locus_tag="PC407_000484"
     CDS             1383..2555
                     /locus_tag="PC407_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2495964.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGKVALIKAIKVLAMCGVDILYSK"
     gene            2599..3534
                     /locus_tag="PC407_000485"
     CDS             2599..3534
                     /locus_tag="PC407_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HDF2495965.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(4333..4731)
                     /locus_tag="PC407_000486"
     CDS             complement(4333..4731)
                     /locus_tag="PC407_000486"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDF2495966.1"
                     /translation="MKWKELYNNARGKLNPRTISPFIDAGGVAAAILTDSGNVYTGVC
                     IDTACTLGMCAERNAIANMITNGESKITKLVCVMSNGSVGSPCGACREYLMQLDKDSA
                     CIEILVNVETEKTVKLSELLPDWWGSDRFI"
     gene            complement(5030..7681)
                     /locus_tag="PC407_000487"
     CDS             complement(5030..7681)
                     /locus_tag="PC407_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2495967.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQST
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANQG
                     VYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDGGGTPAT"
     gene            7895..8593
                     /gene="yunB"
                     /locus_tag="PC407_000488"
     CDS             7895..8593
                     /gene="yunB"
                     /locus_tag="PC407_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HDF2495968.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(8805..9812)
                     /locus_tag="PC407_000489"
     CDS             complement(8805..9812)
                     /locus_tag="PC407_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF2495969.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNSEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISNLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            10040..10924
                     /locus_tag="PC407_000490"
     CDS             10040..10924
                     /locus_tag="PC407_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2495970.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDKKNKIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            11126..12676
                     /locus_tag="PC407_000491"
     CDS             11126..12676
                     /locus_tag="PC407_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2495971.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKEKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            12705..13340
                     /locus_tag="PC407_000492"
     CDS             12705..13340
                     /locus_tag="PC407_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDF2495972.1"
                     /translation="MKKFLNVLFSVCISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSEDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVFLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPNLDPVINILPEEFFFHSGMMILILILLASILLFVMYRLYKSFKIK"
     gene            complement(13451..13711)
                     /locus_tag="PC407_000493"
     CDS             complement(13451..13711)
                     /locus_tag="PC407_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HDF2495973.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCMLPHKKDNYNYIMGKFQDLVKITSKK"
     gene            13928..15211
                     /gene="hflX"
                     /locus_tag="PC407_000494"
     CDS             13928..15211
                     /gene="hflX"
                     /locus_tag="PC407_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2495974.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTSESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGENGITLDVNFDEEDFNIYREYILEK"
     gene            15219..15896
                     /locus_tag="PC407_000495"
     CDS             15219..15896
                     /locus_tag="PC407_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDF2495975.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEAGEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            15982..16425
                     /locus_tag="PC407_000496"
     CDS             15982..16425
                     /locus_tag="PC407_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2495976.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTNCVLAPEFQE
                     GGFYKVKDALDMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            16731..17375
                     /locus_tag="PC407_000497"
     CDS             16731..17375
                     /locus_tag="PC407_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDF2495977.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVVVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITNGTGKIILGEEFYLSEQNGEILI"
     gene            complement(17461..17838)
                     /locus_tag="PC407_000498"
     CDS             complement(17461..17838)
                     /locus_tag="PC407_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2495978.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(17838..18224)
                     /locus_tag="PC407_000499"
     CDS             complement(17838..18224)
                     /locus_tag="PC407_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2495979.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            18403..19158
                     /gene="nadE"
                     /locus_tag="PC407_000500"
     CDS             18403..19158
                     /gene="nadE"
                     /locus_tag="PC407_000500"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HDF2495980.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYRAEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            19241..19960
                     /locus_tag="PC407_000501"
     CDS             19241..19960
                     /locus_tag="PC407_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2495981.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(20128..21132)
                     /locus_tag="PC407_000502"
     CDS             complement(20128..21132)
                     /locus_tag="PC407_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2495982.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            21398..22294
                     /locus_tag="PC407_000503"
     CDS             21398..22294
                     /locus_tag="PC407_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HDF2495983.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            22432..23694
                     /locus_tag="PC407_000504"
     CDS             22432..23694
                     /locus_tag="PC407_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495984.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            23770..24963
                     /locus_tag="PC407_000505"
     CDS             23770..24963
                     /locus_tag="PC407_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF2495985.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LKRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            25592..26386
                     /locus_tag="PC407_000506"
     CDS             25592..26386
                     /locus_tag="PC407_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2495986.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            26480..27820
                     /locus_tag="PC407_000507"
     CDS             26480..27820
                     /locus_tag="PC407_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2495987.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            27957..29189
                     /locus_tag="PC407_000508"
     CDS             27957..29189
                     /locus_tag="PC407_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF2495988.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            29229..30371
                     /locus_tag="PC407_000509"
     CDS             29229..30371
                     /locus_tag="PC407_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2495989.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGTDIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGIGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASSTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            30382..31176
                     /locus_tag="PC407_000510"
     CDS             30382..31176
                     /locus_tag="PC407_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2495990.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFAPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            31187..32209
                     /locus_tag="PC407_000511"
     CDS             31187..32209
                     /locus_tag="PC407_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2495991.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELEVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEVLPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            32308..33684
                     /locus_tag="PC407_000512"
     CDS             32308..33684
                     /locus_tag="PC407_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2495992.1"
                     /translation="MKEQWYKDIFARILSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKDKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(34016..34360)
                     /locus_tag="PC407_000513"
     CDS             complement(34016..34360)
                     /locus_tag="PC407_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2495993.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            34848..35858
                     /locus_tag="PC407_000514"
     CDS             34848..35858
                     /locus_tag="PC407_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2495994.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEESTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            35994..36482
                     /locus_tag="PC407_000515"
     CDS             35994..36482
                     /locus_tag="PC407_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2495995.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            36658..38586
                     /locus_tag="PC407_000516"
     CDS             36658..38586
                     /locus_tag="PC407_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2495996.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFILSNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            38761..39171
                     /locus_tag="PC407_000517"
     CDS             38761..39171
                     /locus_tag="PC407_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDF2495997.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            39193..39495
                     /locus_tag="PC407_000518"
     CDS             39193..39495
                     /locus_tag="PC407_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HDF2495998.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(39711..40403)
                     /locus_tag="PC407_000519"
     CDS             complement(39711..40403)
                     /locus_tag="PC407_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2495999.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMMAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(40429..41616)
                     /locus_tag="PC407_000520"
     CDS             complement(40429..41616)
                     /locus_tag="PC407_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2496000.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRIPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSISYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            41952..43829
                     /locus_tag="PC407_000521"
     CDS             41952..43829
                     /locus_tag="PC407_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2496001.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKTVYDEVIKIVPQSNDVGKKNESQEKQSIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSFIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            44141..45547
                     /locus_tag="PC407_000522"
     CDS             44141..45547
                     /locus_tag="PC407_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2496002.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNTVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            45575..46423
                     /locus_tag="PC407_000523"
     CDS             45575..46423
                     /locus_tag="PC407_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2496003.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            47084..47776
                     /locus_tag="PC407_000524"
     CDS             47084..47776
                     /locus_tag="PC407_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2496004.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            47773..48708
                     /locus_tag="PC407_000525"
     CDS             47773..48708
                     /locus_tag="PC407_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2496005.1"
                     /translation="MKMDEQTIILFLFLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVDDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTVFTVSLPLI"
     gene            48856..49779
                     /locus_tag="PC407_000526"
     CDS             48856..49779
                     /locus_tag="PC407_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2496006.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAHAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            49763..50533
                     /locus_tag="PC407_000527"
     CDS             49763..50533
                     /locus_tag="PC407_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2496007.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVTVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            50543..51325
                     /locus_tag="PC407_000528"
     CDS             50543..51325
                     /locus_tag="PC407_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2496008.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGIAVLLSLFSFLCTFILGLLFCNINISVTLIVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            51702..52238
                     /locus_tag="PC407_000529"
     CDS             51702..52238
                     /locus_tag="PC407_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2496009.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            52270..52659
                     /locus_tag="PC407_000530"
     CDS             52270..52659
                     /locus_tag="PC407_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2496010.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            52696..53082
                     /locus_tag="PC407_000531"
     CDS             52696..53082
                     /locus_tag="PC407_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2496011.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGSQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            53209..54651
                     /locus_tag="PC407_000532"
     CDS             53209..54651
                     /locus_tag="PC407_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2496012.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVENESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLIDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            54948..55763
                     /locus_tag="PC407_000533"
     CDS             54948..55763
                     /locus_tag="PC407_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2496013.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINNFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKRVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT        20603 a         6875 c        10242 g        18191 t
ORIGIN      
        1 atgataggtt atataataaa atcactaaca attatattat aagaaaatta taaaaattac
       61 aaggaggtat agaaatgaaa gaaaaagcag ggattagaaa tgccataata gcagccttta
      121 taatcacaat attggcagaa ttaattggac ctatttcatt taaagttatg ggaataaatg
      181 taactttact tagtatacta tgggctatat tcataggaat ggctgtttct cctcatttat
      241 tagggagagt tataccagcc ttaaaaaagt ttatagggga taatgaaata aatgtatctc
      301 catatttatt atctttgaca ctatatccat taggaataat gtttggaatt aatgcagggc
      361 ctaaagttgg tatattactt caagcaggac cagcactttt attacaagaa tttggtcata
      421 tgggtacaat gttgatagca ttacctgttg gagtagcatt aggtcttgga agaagtgcat
      481 taggtggtac tttttcatta tgtcgtgaca cagcactagg cattattgga gataagtatg
      541 gattaaactc accagaaggt ataggaacac taggaactta cataagtggt agtatatttg
      601 gaacattatt attttcattt ttagcaccag tgggtgtact tttaggattt catccatatg
      661 ctttagcaat ggcatcagga atgggtagtg gttcaatgat gggagctgca acagcatcat
      721 taatgaatac ggttccacaa atgtcagagc agattttagc atattcagct actagtggat
      781 tgattacagc tgttactgga atatatatag aattattctt atctttacca gtagccaact
      841 actattatag caaagtagct ccagttattg aaagatttag aggaagaaaa acaagaagaa
      901 caagtaataa attgtaaagg aggatatata tatggaaaaa acttttaaga ctattatgga
      961 gcagataaaa atagtattgt ttatatatat tattacttgc tttggacaaa tgatagcact
     1021 taagactcag gctcaagatt tgttgattgg aagtatatct ggatttttgt tggtgataat
     1081 tgctattata ataaagaatt ttgttaaaaa acctaattta ccaggttttg catgggctac
     1141 attagttgca ttcgttttat cgcttccaat atctccagtg ggggatatca tagcaacaaa
     1201 tgtagggaaa atcaatttta tgtctacagt aacaccatta ttagcttttg caggaatctc
     1261 tgtaggtaac caattagata tattaaagac tttgagttgg aagttagtag ttatatcatt
     1321 tatagttatg acatctactt actttggttc tgcttttata tctcaaacag ttttaagatt
     1381 aaatggaatg ctttaaggag gtagtaaata tgaatgaatt aaaagcaaaa attaaagagg
     1441 aaataaagtt attatctgaa gagaaaaaga catcatttga aaaagtttct gactatttat
     1501 tttcaaatcc agaattagca tttgaggagt ataagtcaca aaaagcatta tgtgatttgt
     1561 tagaagaaaa tgaatttaat gtaactaaag gtgtaggagg attagaaaca tcttttgaag
     1621 ctgtgtattc aaatggaact aatgggaaaa cagttgcttt tctagctgaa tatgatgcac
     1681 ttccaggaat gggacatgca tgtgggcaca atatcatagg gacaagttct gttggtgcag
     1741 gtatcatatt aaaagaaatc atgaaaaaac ataatataga agggactgta aaggtttttg
     1801 gaacacctgc agaagaaaga gtaggtggaa aaataactat gattaaagaa ggtgtattta
     1861 ataatgtaga tgctgcatta atcttacatc caagtgatgc ttcaatgcct gatgatatat
     1921 catttgctca agttaattta aaatttgatt ttactggtaa ggcatcacat gctgctgctt
     1981 ttccttggga agggaaaagt gccttaagtg gagtaatagc actatttaac agtgtaaata
     2041 gcatgagatt acatcttaaa gattatgcaa gagtacatgg aattataact gatggaggta
     2101 gcatacataa tataattcct gaaaaatcta cagctatatt taatgtaaga gccttaagca
     2161 tagaatattt aaatgagatt tgtgaaatgc ttaaaaattg tgctaaagga gctgcaattt
     2221 ctactggaac taatgtagaa atagttcaat tagatgaaat atacaaagag attaagaatg
     2281 attctgaatt agtgaatata gttagacaaa attttgaagt tttgggtgaa gactatgttg
     2341 aaagagattt aagtcaaggt ataggttcaa ctgatacagg aaatttaact catgagatac
     2401 ctgcaataca agcatatata aaattaaaag aaaatactgc aactcataca gatgaatttg
     2461 ctgttgcagc tggtggtgaa gaaggcaaag tagcacttat aaaagcaatt aaggtgctag
     2521 caatgtgtgg agtagatatt ttgtatagta aataatatat aattttaaaa tatattaaga
     2581 aaggaaatcg gattagacat gatattaaaa caagtaatag aggtttatga cattttagat
     2641 aaagcaaatg ctaatgggga agaagtaaaa acttacttac aaggttatgg tgaagtagat
     2701 gtaactgtaa aagaattgtc ttcaagtaaa ggttcaacag atttagtaaa gcttacaatt
     2761 cctgggaaaa atggaaaatt aaaaggtgga gatgctccta ctcttggtat tttaggtaga
     2821 ttaggaggga ttggagcaag accagaagtt ataggttttg tctcagatgg tgatggagca
     2881 ttggtagcaa ttgcagtggc tgctaaactt ttggatatgc aacgaaaagg ggacatatta
     2941 gatggtgatg ttgtaatttc aactcatatt tgcccagatg ctccaactaa agaacactat
     3001 cctactcctt ttatggattc tccaattgat atgatgacta tgaatgaaaa tgaagttgat
     3061 agtagttgtg atgcaatact ttctatagat actactaaag gaaatagagt aatcaatact
     3121 agaggatttg caatatcgcc aactgttaaa gaaggctaca tattaaaaac tagtgataac
     3181 cttttagata ttatgcagac tgtaacaggg aaatctccat ttgtattccc tttgtctatt
     3241 caagatataa caccatatgg aaacaatctg tatcacttaa atagtatttt acaaccagca
     3301 gtagctacag atgcaccaat agtaggagta gcaattacaa cagagatgcc tgttgctgga
     3361 tgtgctacag gtgcaactaa ttttagtgat ttagattcag ctgggagatt tgcaattgaa
     3421 gttgccaagc tatttggtag aaataagtgt gatttctatg ataaagaaga gtgggaaatg
     3481 cttattaaga gatatggcaa attaaataga tttcaaacat ttggtataca gtaattagaa
     3541 aaatattaag tttatataat ttgtgataca actatttaag aagatataaa caattaggaa
     3601 taaaagtaag aataaagcac ttagttaaaa actttaaaaa aaaatattta tttgtttata
     3661 taaaaaggag gattctggtt attgggattc tcttttttat gaatacaaat aatatattgt
     3721 aattataaaa aaaggagcgt ttcaaagtga tttttttaat caccttgaga caactcctta
     3781 tattttttat ttaaattcta ttttttattg actgtatagc tattttcacc atcataaggt
     3841 ggcatgacct tgaacagcgt ggttttgtaa agttgggcac cacaaaacaa aaaatcaaca
     3901 agcccttgtg tggtctgctg acgtgtttac tatactatac tttcccaaaa accgcaagca
     3961 ctatgggagg ctataaggcg gcgaccattc aggggcgatc ccctcgaaac cgggataggg
     4021 ttcccatgtg ggacataata aagagcgccc cggaggtatc aactaccatc cggggcgctc
     4081 tttattatgt cccgtgggaa ccatgcccac gaagcagggc gtacaacgga cagataggtc
     4141 ctaccctcct gacagactgg aatctcaatc ccatcgaaaa caatcataac aatcatggca
     4201 agattatata cactcctgta aaatattttt gtgtgtacca tgaagtactg cttgccacta
     4261 ttgagaacgg ataaaaggga agtacgtatt ataccaataa ggtgaacgaa acaaaatact
     4321 catttgataa agttaaataa acctgtcgga accccaccaa tccggcaaga gctcactcaa
     4381 tttaacagtt ttttctgttt cgacattaac taaaatctca atacaagcac tatccttatc
     4441 aagttgcatt aaatattcac gacaagcacc acatggtgag ccaacactcc cattgctcat
     4501 cacacaaact aatttagtaa ttttactttc gccatttgtt atcatattgg cgatagcatt
     4561 tctttctgca cacattccca atgtacaggc tgtatcaata cacacgccag tataaacatt
     4621 tccgctgtcg gtaaggattg ctgcggcaac cccaccagca tcgataaaag gtgaaattgt
     4681 gcgtggattt aattttcccc tagcattatt ataaagttcc ttccatttca tcaggttgat
     4741 cctcctgttg tatccatata aataatcttt tatacgcctt ttccgcctaa ggcacacaac
     4801 acattactcg cttttttcat tctaatattc gtgcgtgaaa atgtcaaacg gtttacttcg
     4861 tttcattact tcatactgct gttcataacc ctgtttgttc acttgggcac cattcgagca
     4921 ccaatttacc cgtttcaggg ttgtagcgaa cacagcagaa atgctcattt catgggcttt
     4981 tctggctaga aaaaaaattt tatcgctgcc agatatttac tattttttat taagttgctg
     5041 gagttccacc accatcagga ggagtagtag agccaccacc attatttcca ccattatcag
     5101 gaggagtagt agagccacca ccattgtttc cgccattatc aggaggagta gtagagccac
     5161 caccattgtt tccgccatta tcaggaggag tagaaccgcc accattatca cctgtgttat
     5221 tattgttatt attattttct tctggttggt tattgtctgg tttttttgta tcttcagatg
     5281 atggttgaga acttacttta gtaggagcag taccttcaat aaatgctgct ttagagccag
     5341 aagattgctt tcctccatcc tctaaattaa tttttgtaaa atatacattt ttaggaactt
     5401 caaattcagt aaccgttaaa ttagcatgta ttttttccat aattttttcc catagttttg
     5461 cagtactagt acttccatgt ggtacttctc tacgttttat agtgttacca gaatcatctt
     5521 ttctaccagc atcatctcca atataagttg caccaacata ataaggagtg taaccgacaa
     5581 accaagcatc atatgcacta tttgtagtac ctgttttacc tgctacaggc attccctttg
     5641 gaagtgtagc aacaccacca gtaccttcag taattacgct ttctaacata tctgtaacaa
     5701 cataagctac ttcagggtct acaactttat gttcttcagg agcattttta actaataatt
     5761 gaccatcaaa ggtagttata gtagtaaaaa tagaaggttc tacataaaca ccttgatttg
     5821 ccaatgtacc ataagcagca gccatttgaa gtggagtaat accattcgcc ataccaccta
     5881 gtgtcaatga tgggaatttt ctatcacttg cttcagatga acctgagtga ttattcttta
     5941 aagttgttat accaaagttc tttaagtaat ccatcattat atctacacat tcatcataag
     6001 agtctccaag catttcagca gttttgacag ctatcgtgtt tgaagattta gcaagtgctt
     6061 tacgtaaact catagaacca tgaccagcag tagtagttct tggattccat ttattatttt
     6121 cttcaaactt atatcctcct cgagaatcac ttagagcagt tgattgtgtt atttttaggg
     6181 tatcaatagc aggtgtatat attgctaaag gttttataga agaacctggc tgatgagggt
     6241 tagtagcacg attcaaagtt tttctaccac ttatatttct tcctccagct agtgctctta
     6301 tttcaccatt tttataatct aagattacca ttgcagcttg tggttggcta actccatcag
     6361 ggccgaccat atgtcctggg aaattgctat ttctatcaaa ttcttcttct aaagcatctt
     6421 gcattttagg gtctatagtt gtgtttacaa ttagaccacc attgtagaat aagttttgag
     6481 cttcatcatt tgtatgtcct tgttccatta aagcatttat aacttcactt tcgataaggt
     6541 cttctacaga tgaagaaact ttatctgatg attgaggcaa ttttatttct attttagctt
     6601 tgattgcttc atcatattca ctttgtttta tatatttcaa ttctaacatt ttctttaaaa
     6661 caatttcttg tcttttttta gcttcagggt tacttacagc ctttctaaca actaatgtac
     6721 ctgcttttaa ttgtctataa gtatcattag atataagctc ccaagatttt attttttcaa
     6781 tcatatcaaa gtccacttga gtagggtcat ctaaatcatc atcagttgtg ttaacaaaaa
     6841 ataataattt attttctaaa tcctccttag tttcatttcc ctctaactta gaagttttat
     6901 aggctgaaaa tcttgatgga tttttagttg aaccagcaag aagtgctgac tctgctagag
     6961 tgaggtcagc tgcagactta tcaaaataac cacgtgcacc agcctcagca cctgctagac
     7021 ctctaccaac gaagaagtta tttaaataag tttctagtat tttatcttta cttactgttt
     7081 tactcatctc atgtgcatag taaatatcct ttattttacg aggtatagtt tgctgttcag
     7141 ttgttaacag cattttggat acctgcatag gaatagtact accaccttgc ttagtacctg
     7201 taagggtctt taatactgac ctaaataaac ctttaatatc gacaccctta tgctcataaa
     7261 aacgctcatc ttctattgca attacagcat gttgaagata aggggaaatc ttatctaatg
     7321 gaacgggtgt ctttttattt acacttggag ctgtagacaa tgtttttcca ttagcgtatt
     7381 taatagtagt tgtttggttc gtctgtttat ctaaaacagc ttctgtaact ggagttacat
     7441 ctcttagtga cgcaaatact agaccagcag tacctgctgt accaacaact agtaaaacta
     7501 aaaatactat cccaaatact cttagttttt taaatttatc gcttttttta gattttttct
     7561 tgttttttgt tgaacgagtc ttgttagctg aagttctatt aattggttta tttgtattac
     7621 cagatgagct aacttttttt ctcctgattt tatttccatt tttatcatta tcgttattca
     7681 taagatttta acctccttac ttaatgcaat attataacac aaataggagt agttttgtaa
     7741 ttaatagaaa aaatgaattt gctataatat tctgtacatt gtatatgtat aaacgtaata
     7801 tattaaaaag tctatatttt tattttattt atttttgaca aatattataa aaattatgcc
     7861 aaagctacat ataaataaat aggaggggat aattttgaat agaatagtag atagaaaaac
     7921 aaaaaatgaa tttagaaaaa tcatagctat ttttttagta attcttttct tgtctgtatt
     7981 tataggcagt tttatatata tagataaaac tttaagaccg acaataacag ttttagcaga
     8041 gactaaagct cttgaactag caaataggtc gataaataaa gctgttgctg agatggtaga
     8101 aggaaaaata aattatgaag atttaatgga tatacaactt gataataatg gtaaaataac
     8161 tatgatacaa gcaaacacta taatgatgaa tgaaattgca tctgcaatag ctttagaaat
     8221 acaagatgaa ttaaaaaagg ataagacagc aagttcttac atacctatag gaacagcatt
     8281 aggaagtcct atattagcta aatacggtcc taaacttgaa gtgtctattg aacctatagg
     8341 aacagtatca gtaaacttta aaacggaatt tgagtcttca ggtataaatc aaactagaca
     8401 tagaatatat ttagaagctc aaacacaagt taaagtagta ataccactta taacttcaac
     8461 aaaacaaatt aaagctcaaa tacctatttg tgaaactata atagtaggtg atgttccaga
     8521 aagttatgta aatattccag aaaaaaatct aggaaatgtt ttacctaaca ctggaaagaa
     8581 taccaacaag taatataaag ttgtatattt ttaaataaat tcaaaataat agtttataaa
     8641 taaaattttt ggaaataata ttaatataat tttaataaaa atttatattt tatactatgt
     8701 aaaatatcta atatatttgg tctgtaggtt aaataaaagt acctttagac caaatatatt
     8761 attaataact taataacatc ttaaaactta tttatttata ttatttacaa catccattta
     8821 ataaatacac cataacctac agctgggtca ttttcaattg catgtgacac tgctccgatt
     8881 attttattat cttgaactat tggagtacca ctcatacctt gtacaatacc accagtttga
     8941 gctaaaagct ttttatctgt aattttaatt ttaaaacttt tactttctgg atgtttttgg
     9001 ttttctatat ctataatttc aatattatat tttttacact tattaaagtt atcttgtaaa
     9061 attatttgag cttttcctaa tttaacttca tctaaagatg ctactttata tttttttaaa
     9121 ttggaaatat caaagttatt aatttgacct cttattccaa aatcagtatt ttctgtaaag
     9181 ctaccaataa tattttctcg ttttgcattt atacatccaa cactaccttt ataagatttt
     9241 tctatattaa ggtgcttagt tgtagaaatt gaacctgttt taataggtat ttttcttgca
     9301 ttaccaacac ttattggatg acctacagct ccaaactctg atgtatctgg attgatatac
     9361 gttaaagtag caaatccaga aagtaaattg ttaaaactaa ttttttctaa tatgtgtttg
     9421 gtagttttca ttgttataag ttgtccacct ctatttaata aaatagatac ttgtttttct
     9481 tctggtaaag catttaaggt attggcaaag tcactatatc catcaacttc agaattattt
     9541 acctttacta tagcatcacc caatctaaat ggagatgctt ctccaggatt tctaactatg
     9601 atatttttta attctgcgtc aatttgaagt acgtttccaa ttggtattaa atattcaggt
     9661 tcattttttt gtaaattttg tgcataaatt aaattatttg agaaaaaata taataaaaat
     9721 aaaaatgtta aaacaattgt aaattttgac ttagctaagt tggttttttt acttaaagta
     9781 ttatttttaa aatgaaaatt tttaagttgc ataaaacacc tctttcttat taaattattt
     9841 taataataga attgaagttt tcttgctgct tctatattat gttgtgcaag aattatgttt
     9901 tgatgcttgg aaaactgact caataacacc ttagttttca taaaaataaa taaaataaat
     9961 agtgtatatg ataaaataaa tacatattca tataaataaa aataaacaat aactgtctag
    10021 cataagaaag gagcaaaaaa tgaaaaaata tagattgtta gttacttgta taattgccat
    10081 gggaatcata attataggaa catcaaatcc agttagcaat ttttacaaga ctacaaattt
    10141 gattagttca ggaaatatca caaatgaaaa agcagataaa gatgaaaatt caaacaaaaa
    10201 taataaagat aaaaatgaag acaaatctaa ttctaaaagt aataaagata agtcatcaga
    10261 ggacaaatcg tcagataaaa aaaataaaat tcaaaaaaat aaaaaacttt taatttgtat
    10321 agacccaggg catcaaggaa aaggagatag caatttagaa ccagtagctc caggttcttc
    10381 ctctaaaaaa gcgagagtat cttcaggaac agagggtatt gctacaaaaa aaccagaata
    10441 tgttctaaac ttagaagcat ctcttgtttt aaagagtata ttagaatcta aaggttataa
    10501 tgtaataatg actagagaga cacatgatgt aaatataagt aattcagaga gagcaatact
    10561 tgctaatgat aaaaaagcag atatggttgt aagaatacat gcagatagtt taaataattc
    10621 ttcaaaaact ggagcatcta tattgattcc tgaaaaagat ggaaaatata cagctcctat
    10681 atatgaagag agtaataaat gtgctgaatt tatcaaacag aatatggaac aatcaggtat
    10741 acaaatcaat ggaatcgttc aaagaggtga tttgactgga tttaactggt caaaagtacc
    10801 agccgtatta gtagaaatgg gatttatgag taactataat gaagaccaaa tgatgtcaaa
    10861 tccagactac caaagaaaaa tgatgcagtg tatagctgat ggattggatg cgtattttaa
    10921 ataagatatg aatagagaaa aagttaggtt ggtattattt tcctaatttt tatctctatt
    10981 ttttattgta cataatgcaa agaagaagtt tattttaggt taaattgtcg taaatataac
    11041 taaaaatatt gggaatactt gatttaagtt taaaaagaat atattatagt atagaatgtg
    11101 taaaattttt ttgagaggtg ttgaaatgct agtagtagaa aatgtgagtc atggatttgg
    11161 tgcgagaaca atattagaaa atgtatcctt tagacttaga aaaggtgaac atattgcact
    11221 agttggtgct aatggagaag gaaaatcgag ttttttaaat ataataacaa agaagcttat
    11281 gccagatgct ggaaatataa aatggtcttc aagagctaca gtaggttact tagaccaaca
    11341 tacggtatta agcaaggaaa aaactataag agaagtgctt agagaggctt ttaagcatat
    11401 gtttgactta gagcaagaaa tgattgctat gtatgacaaa atgggagaag ctagtgatga
    11461 tgaaatgagt aagcttcttg aagaaacagc agagatacaa actatacttg aaaatagtgg
    11521 tttttatatg atagatgcaa agatacaaga agttgcaaat ggtcttggtc ttggagaaat
    11581 tggtcttgat aaagatgtaa ctgacttaag tggagggcaa agaacaaaag tacttcttac
    11641 taaacttcta cttgaaaatc caactattct tatattggat gagcctacaa actatttaga
    11701 cgaagagcat ataacatggc ttactaagta ccttcaagag tatgagaata gttttgtctt
    11761 agtttctcat gatattgaat ttataaataa tacatgtaat gtaatatatc atatggaaaa
    11821 tggagagtta aatagatata aaggaaatta tgatgaattt gtaagactta atgatataaa
    11881 gaaacgtcaa gaagaacaag cttatgataa acaagttgaa gaaagaaaaa gactagagga
    11941 ttttgtagct agaaataaag ctagagtagc aactagagga atggctaaca gtagacaaaa
    12001 gcaacttgat aagatggaaa tattagaaag accaaaagaa aaaataaaac ctacttttgc
    12061 ttttaaagat gctagagcag ctagtaaaat aatttttgaa acagagaatt tagttttagg
    12121 gtatgatgaa gcattgacaa aaccacttaa tttccattta gaaagaggaa aaaagattgc
    12181 cttaaaagga atgaatggta ttgggaaatc tactctatta aaaactttac ttggaataat
    12241 aaaaccattt gaaggaaatg ttaaattagg tgactatttg gaagttggat attttgaaca
    12301 agagagttca agggaaaata gtaatacacc tatggatgaa gtttggagtg aatttccagg
    12361 gcttacaaac tttgaagtta gacaggctct tgcaaaatgt ggacttacaa atgaacatat
    12421 aacgagtcaa atgagagtat taagtggtgg agaagctgct aaggttagac tttgcaaagt
    12481 aatgttaaaa aatataaatt tcttagtatt agatgagcct acaaatcatt tagatgttga
    12541 ggcaaaagat gaattgaaga aggctatcaa agagtttaag ggaacagttc ttttagtatg
    12601 ccatgagcca gaattttatt cagaaattgt tgatgatgta tggaatatag aagattttac
    12661 cactaaaatc gtgtagtgat ttaacttatt gctgggggga tttcatgaaa aagtttctaa
    12721 atgtactttt ttcagtttgt attagtattg ttataatagt aggtgttatt aattttactg
    12781 ttggatttaa acaactttat tattttgaca ttgattactt aaatatatca gagttatctg
    12841 gtttatctga agatgatata aaattgaact acgattatct aattgattat aacttaaata
    12901 agaatgtgtc tgagttcaag ttaccaacat taaaatcttc tccacaaggg aagatacatt
    12961 ttgaagaagt caggaatata tttcaaaata taaataaatt agctaaatta ttattggtag
    13021 tttttttagt aggaattata ttgagtgtaa aaaataaaaa tataaaaatc ttgaaaacta
    13081 catcaataac acttataatt atgccattat tattaacagt tccaatttta ttaaattttg
    13141 aaaaatcatt tattattttt cataaattat tatttagaaa tgattattgg atatttaatc
    13201 caaatttaga cccagttata aatatattgc cagaggaatt tttttttcac tcaggaatga
    13261 tgattttaat attaattttg ttagcaagta tcttactatt tgtaatgtat agattatata
    13321 aatctttcaa aataaaataa gatagcaaaa acgaattata ttaaaggtta tacaataaaa
    13381 atagctatgt aataatattt aaactaagag tagtcgataa atatttatgc atagctattt
    13441 ctaatagaaa ttacttttta ctagtgattt taactaaatc ttgaaatttg cccataatgt
    13501 aattgtaatt atcttttttg tgaggtaaca tgcaactact gcaatctttg atacctttat
    13561 ctgtatactt aaagtttcca ccacagtttt ctcctaaagc atacaaagga caatagcaaa
    13621 atagacagtt aaactcttct ggtttattag ttttatgaca agggaaaaat tcgcaatctt
    13681 tgtgattaaa aaacttataa ttttcgccca tattttcctc ctaattaaat aaaatttaaa
    13741 ttttttatac tatataagat tttggtgata atataattat actaaataat tttatgaata
    13801 gtcgataata tagtattatt aatcatagta ttaatggtat aattagtcaa cagaaaaata
    13861 atgtaaaaat tatgtaagtg tattttgtaa ttagaatatg ttttataaca aaaaatgagg
    13921 tgattaagtg aaagaaattc aagaaaaagc acttcttgta ggtctgaatt tgactacaat
    13981 agtgaaaaaa aatgatgata tagacactag tgaatctatg gaagaattaa aagagctaac
    14041 aaaagctgct ggggcagaag ttgtaggtag cttaattcaa aataaacatt ctgtagatgc
    14101 agcttattat ataggaaaag gtaaagtaga agaaataaga gcctatagtg actctttaga
    14161 tgctacttta gtaattttta atgatgaatt atctggtgct caaatcagaa atatagagaa
    14221 tgttgttgga agaaaagtaa tagatagaac tactttaatt ttagatatat ttgctcaaag
    14281 agcacttagt aaagaaggta aattacaagt tgaattagca caattaaaat atagattgcc
    14341 acgactttat ggaatgggtg gtgagatgag tagaacaggt gcagggattg gtactagagg
    14401 tcctggagaa caaaaactag aaatagataa gagacatata cttaataaag cagcagacat
    14461 aaggcgtgaa cttaaagaag taaaaaagaa tagggagaca cagagagtta agcgtttaaa
    14521 gtcaaatata ccgattgtag cacttgttgg gtatacaaat gcaggaaaat ctactctttt
    14581 gaacgaatta ataaagacac acaaggatta tgaacaagaa aaagaagttt ttgtaaaaga
    14641 tatgcttttt gcaactttgg atgtaactct aagaaaggct cttttgccga ataaaaaaga
    14701 atttttagta gtagatactg ttggatttgt aagtaaactt ccccatgatt tagtagaggc
    14761 gtttaaagca acattggaag aagtacagta tgcagattta attttacatg taattgatgc
    14821 gacaaatact agttatgaat tgcaaaaaag tacgactgaa ggtgtattaa aagaacttgg
    14881 tgtaaatgat aaaaaacata tacttgtata taataaagtg gataagctag aattggatat
    14941 atatcctaaa agtcaagaag atattgtgta tatttctgca aaacaaggca taaatatgga
    15001 taagctatta aatatgatag aaattgcttt aatggaaaat acttattcag taagcttgat
    15061 gttaccttat gagagaggtg atatatttag tagaataaaa gataaatata atgtggagaa
    15121 ttttgagtat ggggaaaatg gcataacgct agatgtaaac ttcgatgaag aagattttaa
    15181 tatctataga gagtacatac tagaaaaata ggttgaatat ggatataaat tgggaaaata
    15241 tagataatct tgaagattat tttataacct atcttcttta taaagaatct aaaacagtat
    15301 ctcaaattag taaaattaga aatatttcaa gtactgaagc aggtgagcaa cttatacaag
    15361 caaaattaaa aataaaggaa atgcaaaaag atgattttga ggcatctaag gatattttag
    15421 ataaattttt agaactagat aaaattaaga gacttgattt tatggatagc ttagatgacg
    15481 aaaaaatggt atactttaag agaaaggttt tcaaaagaat ccttgtggaa aaaaatgctg
    15541 aagatttaat tgttttaatt tgggcgacag gtgagctaaa agatgataga ttcttaaagt
    15601 tgcttcatca gctcacaaac cacagacact cagatattag aagaataact tattcagcaa
    15661 tcagaaagat tgaatcacca agtagtagag aagtactgca aaaaggtctc tacgataaaa
    15721 atgcacaaac tagacagtat tgtgcaaagg ctctttcaaa actaggggat gaaaacagtt
    15781 taaagatttt acagcagttg aaagacaaga ataagaattt tgaaaaagaa tatgtgctta
    15841 gagcttatga cgaagccatt aagtcattgg aaaatgcaaa aaatttgaaa aagtaatttt
    15901 ttattaaagc aatttgttta cttaatgatg tatttctaga gtttaacaag gtaataaata
    15961 aattaatggg aggggtttca aatgatagta agacgttgtg caggtggagt agtgttttat
    16021 gctaataaag tacttattgt aaaaaatgat aggggagaat ggacacttcc aaaaggaaag
    16081 atacttggag gaggacttcc atatgagagt gctgttcaaa gagttaaagt agaaactggc
    16141 atagatgcta aaatgataga tgtggctggc gatactatgt atgaattttt ctcaagaagt
    16201 agacaacaag aagtttgtaa tgcaataatg tggtatgtta tggaagcttg taacactaat
    16261 tgtgtacttg ctcctgaatt tcaagaaggt ggattttata aagtaaagga tgctttagat
    16321 atgttatcac atcataaaga gcaagcttta gtagaagtat cttataaaaa gtttaaggaa
    16381 ttaaagaaat tagtttcaga gggcgatgat gttccagcaa attaattgtg aatttagagg
    16441 attctgagga agggatagcc ccttcctttt ttaattgaag cagttttaag cgtaaattat
    16501 atagtctctt taaattatga tttatcaatt tagagcttta gtagtttata tttaaaatca
    16561 gtaagatata tttaaaataa aaattaaaaa gtatacaatt ttattatata attgtcttat
    16621 gatattgatt aaagaagtat taaaagtcca tttattttta aatagaataa ttttatagat
    16681 atataatttg atgtgtatat tagaaaatga ttaaaatagg aggaaataca atgagcaatt
    16741 acaggacatt acatgaattt ggaacagatg aaataactat tgaaaagtct gtttttattg
    16801 gatatgctaa gcctataaag agtgaagaag aagcattaga gtttataaat gaaataaaga
    16861 aaaagcataa agatgcaact cacaatgtgt gggcttatac tgtaggtaaa aatatgaata
    16921 tacaaagata tagtgatgat ggagaaccac aaggaacagc tggaatacct acattagagg
    16981 ttataaaaaa agaagatttg agagatgtag tggttgttgt tactaggtat tttggaggaa
    17041 caaaattagg tgcaggcggt ctagttagag cttatacaaa aggtgctaaa ttgggacttg
    17101 aagcaggtaa gataatttat aaggtaatgt atcaagaagt taaggttaag atagactata
    17161 ctcaacttgg aaaagtgcaa aatgagttaa tgaatttggg gtattttata aaagatactg
    17221 tatatgagga taatgttgag atagtagtgt attcaagatt agaagatgta gagaaattat
    17281 cagaaaaaat gattgatata acaaatggaa caggaaaaat aattttagga gaggaatttt
    17341 atttgtctga acaaaatgga gaaattttaa tataatctaa aaggttatta gaatagttaa
    17401 tacctcaaat agccaattag tatttttaag tgaaaagcca ttaaattgat ttaatctaaa
    17461 tcaatttaat ggctttttat cggattttgc acagaatcct ttttttactt caaataaacc
    17521 gactaaaagt aaaaatatcc caaatatttt ttttaatata tctgaagaca gataattagc
    17581 taatagtgaa ccaaatacag ctcctaaaac cccaaacaca gctataggta taaccaattt
    17641 aaaaattaca tttttatttt ttatatgtat agcaagtgct ataagtgtca tagggataga
    17701 tgaaagtaaa tttacgcttt gtgctatttt agtgtctatg ccagcaagta aaacgagtgc
    17761 aggtataagg attgttcctc cacccattcc cattcctcca ataattcctg caaaaaatcc
    17821 aattagagca aataacatta aaacaccatc cttaatgctg caattatcat aattattcca
    17881 aaagaaattc taagaaactt tccagtaacc ttatttagta atttagctcc tataatacca
    17941 ccaagaatac ttccaactgc aactttaatt gttaagttaa catcataagt acctttagat
    18001 acatataaaa cagaacttgc tgtcgttaag aatattatta tagaaagagc agttgcatga
    18061 gatttatgtt cttctacctt aacgatgtca tttaatattg gaactaaaag agtacctcct
    18121 ccagaaccaa aaactccatt tataaatcct gtaaaaactc caattatcgt attttttaag
    18181 ttaaaatcaa agtatttttt ctttaaatca tcagtttttt tcataaaaat caccttctga
    18241 aatagtttta ccaaaatagg caaattatat ttatataaaa taagattaaa tataatcttt
    18301 catgtttgtt taattttgca taaaaaaaaa catattaaaa caacatatta atatatggta
    18361 taatatatta aaacaaagta gcaaatttga gaggagataa caatgtcaaa tataaaaata
    18421 caaatagaca aaactgtaga atggcttata aataaggtta atgaggcaaa tgcaaaagga
    18481 ttaattgttg gtgtatctgg aggaattgat tcggcagtag ttgctaattt aattaaaaag
    18541 gcatttccag aaaactctat gggagttata atgagcataa aaagtaatcc tcaagataga
    18601 gaagatgcac ttaaggttat agagggatgt gatatagaat accttgattt agaccttata
    18661 gaaccacaaa gtgctatatt agacatggta gtaggaaatt taaaagataa acatttatat
    18721 agagcagaat atttaaagat gacagatgcg aacttaagag caagagtaag aatgagtaca
    18781 atttatacaa ttgctaacaa tctaggatat ttagttgttg gtacagacaa tgctgcagaa
    18841 atacacacag gatattttac taaatttggt gatggtggag ttgatatttt acctattgca
    18901 aatttaacta aaggagaagt atatgaatgg gcaaaagagc ttggtgttca tgaagattta
    18961 ataaacaaag ctccttcagc aggtctttgg gaaggtcaaa cagatgaaga tgaaatggga
    19021 actacttaca atatgataga tgctgtgtta gaaggtagat tggatgaagt tcctaaaaga
    19081 gaccaagaga taatagaaag acttcataga ttaagtgaac acaagagaaa aacgcctgca
    19141 caacctccta agttctaatt tactatttat aagatttgtg ataaaattta caagtgatga
    19201 aaattagaat aattaattgt acgtattaag gagtggaaat atgggacgta taggtaatat
    19261 aattaataga aaaggtaaac aagatgctca aagagcaaag atatttacta aacatgctag
    19321 agcaatagca gttgcagcta aagaaggtgg agcagaccca gagtacaatg cagcgttaaa
    19381 aacagctata gaaaaagcta aggcagataa tatgccaaat gataatatag atagagctat
    19441 cgctaaaggt gctggagcag gcgctggaga agactatgaa actatagttt atgaaggata
    19501 tggacctggt ggagttgctg ttatagtaga aactttaact gacaacaaaa acagaactgc
    19561 tggtaacgta agatactatt ttgacaaaaa cggaggaaac ttaggaacta gtggatgtgt
    19621 gtcttttatg tttgataaaa aaggacaaat attagttggt ttaggagatg gagtttcaga
    19681 agaagaattg atggatgttg cattagaagc aggagctgaa gatttcataa cagaagaaga
    19741 tggttatgaa ataataacta caccagaaga tttttctagt gttcgtgatg agttaaaagc
    19801 taaaggatat gagtttatat ctgctgatgt gaagatgata cctcaaacta cgactgtatt
    19861 gactgaagaa tctcatttaa aaatgatgaa taaattagtt gatatgcttg aagaagatga
    19921 tgatgttcaa gatatatatc ataactggga agtagaataa atagtataag catattgaaa
    19981 tttataagca ttgaatcaat tttaacatgt gataaatttt atagaataag aaattactaa
    20041 aatagacata cgcacctcta acctatattt gcataataat atgtaagtat aggtaagggg
    20101 tgtttttata tttataggtt aagaaattta aagtaaattt tgagctggac ttgattgtct
    20161 ataaataatt gatgggtata taacttgttg ctgaatacca tttatacgcc cagttactgt
    20221 atcaataatc atcctaagac tacgttctgc tgtctcttcc atttttaaat ctataactgt
    20281 caaaggagga atacttcttg ccattagtcg tgaaggtcca ttacttgctg caaatatttg
    20341 aatatcctct ccaattacaa ttccagattc atgaaatgca ttcatcatac caagagctac
    20401 aacatcatga ttacaaaaaa ttactttttt tagtgaattt tttgcaagca ttttttgagc
    20461 aatatcatat ccactatcta ttttattaga acaataatat atagaatctg atagatgtat
    20521 tccatgttct ttacatgttt ttaaaaaaga tttacccctt aaagtcatac atgtaaagga
    20581 agttgggttg aatacaacag caatatcatt tttaccattt tgaatagcat gtaaagctgc
    20641 tatacgacca gtttcctcat tatcaataga tacacttgaa aaaccaggta tttcacgatt
    20701 tacaacaact acaggaattt taggtttatt ttttactaga tattctatat catcagcatt
    20761 gatacctgtt aaaacaattc catcataata attatttgca aaaaagtatt catcatcaca
    20821 tagatgacca actttgtaag gtcgtatgat gatatttatg tttaaatgtt ctaaagaaat
    20881 tatgtggttt attgcttgaa gtatattggt tgatgccaac tcaaatccct catcctgcca
    20941 ataaaaagct attttaatat tattatgttt aataggagta gagcgtttat atccaagctt
    21001 atcagctgaa tttaatataa gctcttgtgt atgtagcgga atcctgcgtt catttccttt
    21061 accacttaag acgtacgaga cagttgctga agaaagacca cagtcttttg caatatcttt
    21121 gattgttgcc atgataaaat cctccttaaa tagttgctta aatttgctta taccaataat
    21181 cttaacatat attcaactaa agaacaatat tctacaaggt gcatatttta caggattaga
    21241 ataaataaat ttaaaaaaac ttcttactta tgttgacata atttagttta taggattata
    21301 atatgcttaa ataagcttat tattaactca aattaagcaa agttaagcag gaacatgcaa
    21361 tgctagcttg caatgtgact gtaaaggggg aaaaggtatg agtaaggtaa atatatttga
    21421 agttggtcct agagatggat ttcaaaacct gaaagagttt ttagaaactg ataaaaagct
    21481 taagattatt gatggtctta ttgcttcagg gattaaacaa cttgaaatta gttcatttgt
    21541 aagcccaaaa gcaatacctc agatgaaaga tgcaaaagaa gtagcaactt attgtgtaga
    21601 aaaatatcct gatgtgaaac tttatgcatt agttccaaat ttatatggtg ctaaagttgc
    21661 atgggagtgt ggtataagag atatatccta tgttatatct ttgagtgaaa ctcataataa
    21721 aaaaaatata aatcgtaccc atgaagagtc tattgatgag cttaaaaaaa taatgaatac
    21781 ttatccagac atgaatatat gtattggaat ggctactgta tttgggtgtc cttttgaagg
    21841 tttaccaaaa attgaagatg ccataagatt tgctaaacgc ctttgggaaa acggtatcag
    21901 ggagataaat ctagcagata caatagggat tgctgaccct aagcaagtaa gagaaactat
    21961 tagtgcatta gttgaagctt taccagagtg cagatggcag gtacatattc atgatactag
    22021 aaatatggga atggttaata ctcttgtggc tattgaatct ggaattacta ctgttcagtc
    22081 tacacttgga ggattaggag gatgcccatt tgctcctgga gcatctggca atactgcaac
    22141 tgaagattta gtatatatgc ttgaacgtat ggggtatgat acaggagttg attttgttaa
    22201 acttcttgaa ttatcaaagt atcagaagag ttttgtaaat ggagtatata gtggtcatca
    22261 tgtaaatata aagtctgata gttgtaattg ttagttctat tgattaatcg attaaaaaaa
    22321 tatgttgatt aaaaaaatat gctgattggt aaaatagatt tattgataaa aaagataaat
    22381 tataaaatac attatctaat aaaacaaata aaaaaattga gggggattag tatggatatt
    22441 atagctttac ttttattttt aggagttatt attattgcat ttgtacgtaa gaataatgca
    22501 ggtattttgg ctctagctat tggtgtcata gctgttagaa tttttggaat ggatgataaa
    22561 gaactttatg gagctataag caactcaatg tttactacac ttgttggtat aacactactc
    22621 tttgcaatag tgaactcaac aggagcatta aagcttgttg cagaaaaaat tgtagctgct
    22681 tctggtaaac gtgtttggct tatacctata gcagttttta tagcaggatt tgttgttgct
    22741 ggtgttggac ctggggcaat tccagcactt gcaattatcc cagcaatggc tgttcctgta
    22801 gccataacgg ttggttataa tcctgtaatg cttgcactta taggtgaatg tggattaatg
    22861 gcaggtcgta tgactcctat tactccagaa ggtcaaatta taacaaccgt tgcagaaggg
    22921 gttggaattg aaaatgttat gcctactatc ttagcctgtc aaactctttc tacaattgta
    22981 ttttcaatag tattgtttat aattttcaaa ggatataaac ttaaaaaacc tataaatgtt
    23041 atagatagta agcaaataga aaaatttaat ataaagcaga taatttctct aataagcata
    23101 gtagttatga ttgcttttat cataattttt gatatgaatg ttgggcttgc agcttttgca
    23161 gtttcagctg tacttatttt tttcaatatt gctgatgaag gagaatgtat caaatcaatt
    23221 ccatggagca cgatagtaat ggttcttgga gtaggtgcaa tgatgacaat cgtagatgca
    23281 gcaggaggta ttgacttgat gagtaatgca ttatcatcac tgatgaatag taaaacagct
    23341 actccaatta tgagtatttc agcaggactt atgtctatgg ttagttctgc tttagcagta
    23401 gtttatccaa ctatgatgcc tatgtgtgtt gatatagcta aggcagttgg aggagtagac
    23461 ccactagcac ttattgctgc agtcggtgtt ggaggttctc ttgctggtgt aagcccactt
    23521 tctactggtg gggcattgat tttagcagct atgggttcaa gtaagaaaga ctttaataag
    23581 gaagaacaaa ataaggtttt tgtacagctt ttcattatga gtgcagttgg cttattggtt
    23641 attgcagtag tatcaatatt atcatttaat gcaataacac atttaatgaa ctaattataa
    23701 taactgaaaa taattcatta aatttaattt ttaatattaa tcaatataat ataatttgga
    23761 ggggaagtta tgaaaaaagg agcattaagc aatataaagg tacttgacct tacaagagtt
    23821 cttgcaggtc catattgtac aatgatgttg gcggattttg gagctgatgt tataaaaata
    23881 gagatgccaa gaaaaggtga tgatacaaga ggttttggac cttttgtaaa tggagagagt
    23941 ttatactatg caaatataaa tagaaataaa aaaggtatca ctctaaacct taaatcagaa
    24001 gaaggaaaaa atatattttt agaaatggta aaacatgctg atgttgtagt agaaaattac
    24061 agaccaggtg taatggaaaa gttaggtctg ggttatgatg agttggctaa agttaacaat
    24121 caaataattt atggagctgt atcaggattt ggatgttatg gaccatattc agaacgtcca
    24181 ggttatgata ttattgcaca ggcaatgggt ggtcttatga gtataacagg gcaagaagga
    24241 ggagaaccaa cgagagttgg taatgctatg ggtgatgtac taggtggaat gaatttaact
    24301 attggtatac tcacagcact caacgctaga aaaataatag gaaaaggaca aagagtagat
    24361 gtttccctcg ttgattctgt tgtatcgtct ctcgaaactg gtatacagag atactttgat
    24421 tcaggacaac ctgaacttat gggtaataga tatgcttcag cctatcctta tgactctttt
    24481 aaggcaagtg atggaagttt tgtaataggc tgtggtaatg acaaactttt tgaaaagtta
    24541 tgtaaaaaag ttctttttag agaagacctt ttaatagatg agagatttaa aacaaatctt
    24601 aagcgttgtg aacattacaa ggctcttaaa ccagaaatag aaaaatggtc tgttaagcat
    24661 acaataaaag aagcagttga cttaatatca gaggctggag ttcctgctgc tcctatctat
    24721 aatcttgatt gtgttacaac agatgaacat atagcaaaag caagagaaat gattgtagat
    24781 attagtcatc ctattatagg aaatatgaaa gtcaatggaa atccagttaa gttgatgaca
    24841 acaaaagcta aaattgatat gccagcacca tctctagggg aaagtaacga ggagatttat
    24901 aatggttggc ttggttttga tagtgataaa ataaaagatt taaaagaaaa tggagttatt
    24961 tgattaaaaa atagctaaac acacttgtga ctttagtcat gggaggttca gttagaataa
    25021 tatcaaaaaa tattaaatca atactgtaaa tcaaatatag aattgtcaga agcatttaag
    25081 atgggaaaag ctacttagac attgagtctt tgtggctttt cctattttca ttaagaactt
    25141 tatattatca gaatagatat gtactaaact aaagtattaa caatttaaat taatagaaaa
    25201 taaactacct aattatgttg aataaagact actttagtga aaataaactt atagagatgc
    25261 tatttaaatt attttaatgg aaatatcaaa aatgagactg cattctcaaa aatgagactg
    25321 cgttttttaa gtattttctc gaaaatgaga caaaaatatt gttttatgag ttttattgaa
    25381 gaaaaattct tctgtgaatt acaatttaat caattttaaa gttgaaaatt attaaaaatt
    25441 tagtgaaatt tatgtatatt tcgtttattt tattaaaaat aacaattttt tactaaaaat
    25501 aaagaacttc aatggtgatt ggcatagtac ttgctatttt atattaacaa gaaatgtaga
    25561 aaaaataaca atgtataatg gggggaatat gatggaatct gtaatggaaa atttgaacaa
    25621 tcttaaggtg gaggttaagg acaaagtctg tgttattact ataaatagac ctaaagcttt
    25681 aaatgctctt aatagtgaca ctcttaggga actatcacaa gttattgatg tagtatcaga
    25741 aaatgaggca atattaggag ttattattac tggagaaggt aaagtttttg tagccggtgc
    25801 tgatatacgc cagatgcaaa actataaaag tgaggaaggc agaaaatatg caggttatgc
    25861 tcagggaatt tttgataaaa ttgaagctct tgagaaaaca gtaatagcag cagttaatgg
    25921 atatgcatta ggtggtggat gtgaacttgc tatgagttgt gatattagaa ttgcatcaga
    25981 aaaggctata tttggacaac ctgaagttaa tttaggagtt attccatgtt ttggtggaac
    26041 tcaaagatta tcaagacttg ttggaactgg aatagctaaa gagttaattt ttacggggag
    26101 acaagtaaat gcagaagaag ctaaatctat tgggcttatt aataaagtgg ttccaagtga
    26161 tttactttta gaagagtcta tgaagatgat gaaccaaata gttgaaaaag cgccaatagc
    26221 aataagatat gcaaaagttg ttattaacaa agggatagat atggatttaa agaatgctct
    26281 tgaactagag aaagatattg caggtttaac ttttgctaca agagataagc aagaaggaat
    26341 gaatgcattt atagaaaaga gaaagccagt ttttgaaaat aagtagctat tataactaaa
    26401 ttggaacaat gtagaatata ttcaaaatct aaatttcgtg aaaaaataca tatagtttaa
    26461 ttttaaaagg agttaattta tgaatacggg agtaattata agtttagtag ggattatcat
    26521 agctctatct cttctagttg taatggttat gaagggaata aatattttta ttatagcaat
    26581 tgtatgttca tcattagttg ctataacagg aagcctaaat gtgtatgatg cacttaaagt
    26641 ggattatatg acaggatttg ttggatttct tcaggcaaat ttctttattt tcctaacagg
    26701 gacattaatg ggaaaaataa tggagattac tggtggagca aaatctattg ctaagatgat
    26761 tgtaagatgg attggtaagg ataaagcact actttctatt ccaatagctt gtggaatatt
    26821 agcctatggt ggagtaagtg ttttcgttgt aagttttgca gtgtttccga tagctttaga
    26881 aatttttaaa gaagctgatt tgccacgaag atttatacca gcagctttga cttttggatg
    26941 ctcaactttt gctatggttg caccaggggc accacaaatt cagaatattg ttccagcatc
    27001 aacattaggg acagatatta tggccggagc agtaaatggt tttatatcat gtgcagtaat
    27061 gtttatagtt ggaagtacca ttttgtacaa aatggtttca aaagaaaaag ccaatggtgg
    27121 tcattttata gcacatgaat cagatatatt tgatgaagca gaagcaacta attctaagag
    27181 agaaaatggt cctaatggtg taattgcctt aattccattg atagtgtcaa ttttaataat
    27241 taatgtaaaa gttaatggaa aagcgatagt accaattgag gtgggagtat ttattggagc
    27301 tttattagta tatttattgt taaataaata tcaagataat actaaaatag ttggacatgt
    27361 tggtgatgca tgtaaaacta cagtagtagc aatatgtaat acttgtgctg tagttgcatt
    27421 tggttcagta gtaaaatcag cagttggttt tgacttcata gttaatgcca tgactaatat
    27481 tcctggtcct cctattgcag gggctgcagt tggaactaca gttattgcag gtatttgtgg
    27541 gtcagcttca ggtggactag gaattgcagc tccacttctt ggaccagtat ttttagccaa
    27601 gggtgtttcc gctgcagcat taactaggac tatggcaatt tcatcagcag cattagattc
    27661 acttccacac aatggatata ttgttactgt aacaaatggt ctgtgtaaag aaacacataa
    27721 ggatgcctat atgccaatat tctgggtaac tgttttaact ccgtttattg gaaccatagt
    27781 agcagtccta ctgtttacaa tgttcccggg attgccataa gagaaaagat aaaaggtata
    27841 aattatattt tgatattata aaatcaattt tcaaaatata tacaataaat ttcatgtttt
    27901 aatactataa atatgtattt aaaataacaa attgaccata taaggaggaa ttgaaagtgt
    27961 taaataaacc attagaggga gttaaggtta ttgacctaac gtattttgta gcaggtccag
    28021 ggacttctaa aatattagct gattggggtg ctgacgttat taaggtagag ccaagctttg
    28081 gagacccagg aagaaagaca ggggcaacaa tgacaatgcc aatagatgat tataataatc
    28141 ctttttacag cacatataat tcaaataaaa gaggtttgtc tataaatctt aagagtgaaa
    28201 ctggaattga aattatggat aagcttttaa gtgaagcaaa cgtatttgtt tcaagttata
    28261 gaacaggagc attaaaaaga ctaggtcttg attatgaatc attaagtaaa aagcatccac
    28321 accttatctg gggtcagata aatggttttg gcgattttgg acctgctaag gataatgcag
    28381 gatttgatac agttgccttt tgggcaagaa gtggggctat gcttgatata gcagaaaaag
    28441 atacaagtcc aataaatcca cctatagcct ttggagatgc aacaacttct tgtagtttat
    28501 ctggaggaat atgtgcagct ttgtaccagc aagctaagac tggaaaagga caaaaagtaa
    28561 tggcatcttt atttgggcaa gcaatatgga atgcatcatc tttattagca tctacacagt
    28621 ttagtgatga gtatccaaaa actagaaaaa atgctatttc accagtaata aattcttatc
    28681 gttgtaagga tggaaagtgg atattcttaa gtattttaga gcatgaaaga tattttgagg
    28741 cactttgtag attatttgga agagaagact taataaaaga agagaaattt gcatcttcag
    28801 tagaaagtaa gaaaaatgct acagaactta ttaatatttt ggatggtgaa tttgcaaagt
    28861 ttactcaaaa tgagatggtt gaaaaactta ctgctgctga tatagctcat gaaaaaattc
    28921 aacatgttaa tgatgtagtg actgatgaac aggctattgc taataactat atatatgagc
    28981 atacaagtaa aaatggaaat aagacaatgc ttgcatccac tccagttaaa tttggaaata
    29041 ttgaagttaa catgacttgt gatgctccac ttataggaga acattcagat gagatattga
    29101 gagaacttgg atacagtgat tctgatatag aagctctaat tgaatctgga attgtaacaa
    29161 tgaataaaga agcatgtgaa atatgttaat aaacatgtga agtatgttaa taaataggag
    29221 ggaattttat ggattttaga ttaacagaag cacaattaat gcttcaaaga gtagcaaaag
    29281 aatttgcaga gaatgaaata gcaccaatag ctgctgaaac agataaaaca ggtatttttc
    29341 caagggagct ttttagcaaa atggctaaaa taggatttaa tggcattgga acacctgtag
    29401 agtatagtgg ttctggaggg acagatatag aaaaggtaat tgttgttact gagattgcca
    29461 aaaaatgtgc agcatctgca gcaatattat caatacacac aatatatgct caagctattt
    29521 tgaaatttgg tactgaggaa caaaagaaaa aataccttcc aatgatggct gaaggtgggt
    29581 gtgtaggtgc tttcgcctta actgaaccta atgctggttc agatgctgca agagctgcaa
    29641 caactgcaat tattgatgaa gaaacggatg aatatgtttt aaatggaact aagtgtttta
    29701 tttctggtgg aggtcaagca gaatcattaa taatatttgc actgacagac ccatcaaagg
    29761 gaataaaggg aatgtctgct atcattgtcg acaaaggtac tccaggtttt tcaataggaa
    29821 aaattgaaga aaaaatgggt attcatggtt cagaaactgc agagttaata tttgataact
    29881 gtagagttcc aaagtctaac ttattaggaa aagaaggcaa aggatttaat atagctatga
    29941 cttgtttaga tggagcaaga attggaattg gagctcaagc tgtaggaatt gctgaagggg
    30001 ctttagaaga aagtatcaaa tattctaaag aaagagtcca gtttggcaag ccaatttcag
    30061 cgttgcaagg tattcaatgg tatatagcag atatggcgac aatggtggaa tcagcaaaat
    30121 tattagtata ttatgctgca gatttgaaag ctagaggaga aaaacacact aaagaagctg
    30181 ctatggcaaa atacaatgcg tcaagtactg caagagaggt tacaaatcta gcccttcaaa
    30241 tccatggtgg atatggatat atgaaggatt atccattaga gagaatgtac agagatgcca
    30301 aaataacaga aatttacgaa ggtacttcag aaatacacaa agttgttatt tcaagagcag
    30361 tactaggata ggaggaaaag aatgaatata gttgtttgct taaagcaagt tccagatact
    30421 aatgaagtta aaataaataa agagacaggt actctaataa gagatggtgt accaagtata
    30481 atcaatccag atgatagaaa tgctttagaa gaagctctaa aaatgaaaga tgaactagga
    30541 gcagtgatta aggttataag catgggacct cctcaagcta aaagtgcttt aaaagaagct
    30601 ttggcaatgg gagctgatga agcttattta atctcagata gagcttttgg tggttcagat
    30661 acttgggcta catctacaat tattgctgct gctatagaaa aggttggaaa gtatgatgta
    30721 atattctgcg gaagacaagc tatagatgga gatacagctc aggttggacc agaggttgca
    30781 gaatttttag ggataccaca agttacttat gcaaaagaag taaaggttca agatgacaaa
    30841 ttactagtaa ctcgttatac agagacaggg gattatttaa tagaagcaaa gatgccagtt
    30901 ttattaactg ctataaaaga gttaaacaat ccaagatatc caagtgtaaa aggaatctta
    30961 gaagcttata ataatggaga tgcgaagatt actgtattaa cacttgcaga tttagatgtt
    31021 gatacaactc aaattggatt aaagggttca cctacaaatg tttataagtc ttttgcacca
    31081 gttaaagaca agcataatga aataatagaa ggtataaata aaaaagaaaa agctgagaaa
    31141 ctaatagaaa tactattcga tttgaaacta gtataggagt gttaagatga tgagagcaaa
    31201 agttaatcaa ggtataaatt taaatgatta taatggtgtt tgggtaatag gggagcaaag
    31261 ggaaggcaaa attaatcctg ttacgataga acttattggt gaaggaagaa agcttgctga
    31321 ccaattagga aaagagcttg aagttgtgat agctggatat gaagtagaga aagaagtgaa
    31381 ggaattactt cactatagtg ttgataaagt atattacatc aatgaccctc ttcttaaaga
    31441 tttcactact gatggatatg caatttcaat tgcaaattta atcgaaagaa aaaagccaga
    31501 ggttgtattg gttggtgcta cttctatagg aagagatata gcgcctagaa ttgcaggtaa
    31561 ggttggaaca ggtctaactg ctgactgtac aaaacttgaa atagattcaa cagataataa
    31621 gttattacaa acaagaccag catttggtgg aaatttaatg gccactattg tttgtccaaa
    31681 aaatagacca cagatgtcaa cagttcgtcc aggagttatg gcaaaagctg taagaaacga
    31741 atcagagact ggaattttag aagttgttac tccagaactt actgaaaaaa tgattagaac
    31801 tagattagta gaggtacttc cacaagaaaa gaaatctgta aatctaactg atgctagaat
    31861 aatagtatca ggaggaagag gattaaaaag agcagaagga tttgagctta taaaagaatt
    31921 ggctgataaa ttgggggcag agattggtgc ttcaagagca gcagtagatt cagggtggat
    31981 agaacattct catcaagtgg gacaaacagg aactactgtt agaccagaac tatacattgc
    32041 atgtggaata tctggagcaa tacaacactt agcaggtatg agtgattcta aatatatagt
    32101 tgctataaat aaagatgcaa aagcccctat ttttagtata tgtgactatg gaatagtagg
    32161 agatttatat gaaattatac ctgaaatgat agaatcatta aataggtaac ttttagaaaa
    32221 cattgaatat gtagaatgat aagtcttata atattgtatt gtaagactta ttatttttgc
    32281 tgaaggttat agtggggaga tgaaacaatg aaagagcagt ggtataagga tatatttgca
    32341 agaatattat ctatgactga tgatggtttt attgttgtaa atacaagtgg agtaataata
    32401 gatattaatg ataaatattg tgacttttta ggaaaagaaa gaaaagatat aataggacaa
    32461 aatatacaaa gtattatacc aaatacaaaa atgctagatg ttatgaaaga taagtactgt
    32521 gaagaaggag ctatacatca ttattcaggt ggaaatacaa aagaaaaaag tgtaatagta
    32581 agtaggtctt atgtagaaaa tgacaatgga gaagtagttg caggagtagc tcaagttaaa
    32641 tttagattgc aatcatttga tgtagctaaa aaattaatgt cagaatatat ggagctccaa
    32701 tattataaag agcagtttaa ggataactgc ggttttgaca agctcatagg tgaaaataga
    32761 gacttcatag aacttaagaa aacaggtgtg aaagcttcta aaacaaactt tccagtactt
    32821 ttaacgggtg aaacaggcac aggtaaagaa gtctttgcta gagcaattca caataatagc
    32881 agtaggtcag ataaacctat ggttagtata aactgcgctg caataccaga agaattatta
    32941 gagtcagagc tatttggtta tgacgaagga gcttttacag gagcaaaaaa gggtggtaaa
    33001 aaaggtaagt ttttagttgc aaataatggt acaatctttt tagacgagat aggtgacatg
    33061 cctttgacca tgcaagcaaa acttttaagg gttcttcagg aaagtgagat agaaccagtg
    33121 ggaggtctta aaactataaa gatagatgta agagtaatct ctgccacaag aaagaattta
    33181 agtaaaatgg ttgaagaggg attatttaga gaagacctat actatagatt aaatgtaata
    33241 aacattcata tgatggaatt gaaggacagg caagatgata ttttattgct agcaaattat
    33301 atattaaaca aattaaatgt tgaatacaag gaattgaagg ttttaagtga taaggttaaa
    33361 aattgtttta ttaactatac ttggccggga aatataagag aactacaaaa tgtaataaaa
    33421 agtgcctatg ctgtaagtga tgatatggtc attatgatgt gtgaccttcc atctaagatg
    33481 gataatatca gcagagtagc tcaatgtaat gtggatagca attgttcaat tcatgaaatg
    33541 gttgagaatt atgaaaaaag cttaattatt gatgtgttaa ggaagtataa ttggaaatgt
    33601 tctaaggcag cagaggttat gggaatacac aagagtcttt tatataagaa aataaaaaag
    33661 tatgaaattg aattaaataa ttaagataac ttgaattaag gcaatgctaa aaaaagcgcc
    33721 cttattcaaa gcgaactgta taaactgttt tatgcagtta aaacgtgtaa aagtagatgt
    33781 gaagtactaa tgtcaatcat tagtactttt tattgtattt ataattacta tctatatttc
    33841 agtatctttt gatatctaag attattatgt ttaatttagg atataacaaa accctcttag
    33901 ttattgacag ttttttatat tttaactgtc tactaagagg ggatagttta gtttaaattt
    33961 agagtaaatt ttatctagca tttataaaaa atattttgaa tagctttttt gttatttagt
    34021 cataattttc tacttcttta tcaagttctt tgccacaaga atcacactta tatatagcat
    34081 tgtatccata atcatattca tcaactattt tcttttgtct aaatacacca ccacactcac
    34141 ataggaaacc aatttttata agtgtaacga taaattgtga ttttataaat tttatatcat
    34201 tacatttatc gttagtgtaa aatgtataaa tataagagtt atcgcactca tctcttaatt
    34261 caaatctttc aatatatgtg ttatttttta attcctcaaa ttcttcttta gttaaatatg
    34321 atttttgttg caattcatgt aaacgcttaa tttggtccat tatttatatc ctcccaataa
    34381 ttttagtatt ttaagcttag ataaggaatc taagttttat taaatttatc ccaaggaatt
    34441 atttaaatca tatttagttt tattaattac aacatatata aattacacga aaataaaact
    34501 aattgtaaaa ataatatcct gtgaaaacct aaactactaa ccccttacta acccgaaaac
    34561 taaaaaaaga aaatatatcc aaatataagt gatatataaa taaagatgtt ttatataaat
    34621 ttaactttaa atattctgac aataatatat ataaatatat tatagttaaa atataccaca
    34681 tattttgggc attgtatact ggaattagag taattaattt attttttaac atacaaatga
    34741 ttttatgaaa aaaaacaaga ctcaaaaatt tttatattct ttgatagaat aaaattgaga
    34801 gtaaatttat tgaattttat aaaatttata aggggtgagt ctaagatatg catgaagata
    34861 aaaatgatat atttgaatca atagatactg aggattttga taataaaaca gaagaagaat
    34921 gtttagagct agtaagaaaa aatggattga atttaatata cataaaaaat caaacagaag
    34981 agatatgtct agaggctgtt agacaaaatt gtaatgcaat aaaatgtgtt cagaatcaga
    35041 cagaaaaaat atgtatagaa gcagttaaac aagattggag aatgttagaa tttgtggaag
    35101 agtcaacaga agaaatttgc atgttggcta ttaaccaaga tggaacagca ttaaaatata
    35161 tagaaaatca gacagaggag ttatgcttaa gagccgttga aaaaaatggt gcggctttgg
    35221 agtatgtaaa agaacaaaca gaagaaatat gtatagaagc agttagaaac agcgaatttg
    35281 gattggcaag agtgaaaaat caaacagaaa aaatatgtat ggaagcagtt aagcattgta
    35341 gctataatct aaaatgggta gaaaatcaaa cagaagaaat atgtatggaa gcagtgagac
    35401 aaaatgggct tgatttgaaa tttgtaaaaa atcaaacaga aacaatctgt ttaagagcag
    35461 taagacaaaa tggaatggca ttagaatttg taaaggaaca gactgttggt atatgcttaa
    35521 aggcagtaag acaatatgga atggcattaa aatttgtaaa agaacaaaca gaagaaattt
    35581 gtacagaagc tataaaacaa gataaaagag cacttagttt tgtaaaagga gataaggaaa
    35641 aatacaaggc tctatatgat aataatgaac catttgcaaa gagatatgtt agaaatgtaa
    35701 tagaaaaaga aaacagagca ttgattaaaa aaggagaaga aaatgcaaaa atcaaaattg
    35761 caggaaaact ttatgataga ggcatgtctt ttgaagatat atcagatata gttgagattg
    35821 aaataagcaa gttaaaagtg agtttaggtg ttttttaaag taataaatgt aggtgttttt
    35881 tatgggaaat agttaataaa aaaataatcg actagctctt tgtaggacag caaaattagt
    35941 gatataattt gatgtataat aaattatttt tattagataa gggggaatta aagttgaaga
    36001 ttacagtatt ataccaaaca agaagtggaa acacagaaag ggtcgctaag ctgatagaag
    36061 atggagctaa aaaggtggat ggagtagatg taaagcttat gagacttgat aatattgatt
    36121 tagattattt aaatgaaagt aaagcagtaa tatttggaac acctacttat ttagcaaata
    36181 caacttggga agtaaaaaaa tggtttgatg aagattcaaa aaaagttaat ttagctggaa
    36241 aattaggagc tgtttttgct acatgtgatt atatatgtgg aggtccagat gttgcgatac
    36301 ttactatagt aggacatttg atggttaaag ggatgcttgt gtattctgga ggtggttctt
    36361 taggaaagcc atttatacat ttaggacatg tacattcaat tgaaggtcca gagttacaag
    36421 atgagaaggc tataatattt ggtgaaagaa ttgctactaa ggcaaaagaa ctatttgctt
    36481 aatttctata tgcataataa tgattagagt aaatttaggg ttttaatgga gatttgaagt
    36541 aaaagttata aaataaaaaa ttttccataa aattactcaa gatgaattat aatataatta
    36601 taaataaatt atattattaa aatatttaaa atcaaacgtc aatagcgagg gaagtttatg
    36661 gtatggcata tagaatatga gattttttct gcaattatag ttattttttt aatggtatac
    36721 ttttttagaa gtaaatttat acctacttta caaaacaaaa tatattgtgc acttttaata
    36781 ttctcatttt tatttatact ttcaaatatt ttaggttcat tttgtctaaa taatatagac
    36841 aaaattccaa tttttattac ttttttattg aatcagatat acttattgtt attgccaata
    36901 ccagcagctc ttgtatcatt ttatgttatg gctattattt atcaagatat aaggtatatg
    36961 aaaaaaagat tattattttt atctatacca cttataattt gtataatctt atctattaca
    37021 aatcctttta cgcatattct atttggattg tcaaaagaaa caggttatat acggggttgt
    37081 ggatatgttg caacttttgc atcttttttc ttttatgcaa tatattcagc atttttatct
    37141 tttaaatata gaaaagcaat gcatgaatca aaaatatggg ctattagagg ttttcttata
    37201 gtttcaattg tggcaattat cttacaggca atttttttac agtatcttct tacaggaact
    37261 gcatgtgcat gtagtttact tttgacatat ctttctatgc agaatcgagg tcttattatt
    37321 gatgacctta ctggtgtact caatagacaa tcttttattc aagagctaga tttgaatatc
    37381 aatactgaac aatcaggatt tattattaca attgcgcttg atgattttaa gtttatgaat
    37441 gaaacttttg gaacaaaaaa tggagatatt gcactaaaag aagttggaaa atatttgatt
    37501 cagatacatg atgtaaacca tgtatataga atagggggag atatattttc tattgttgtc
    37561 aatagtcaat taggtataga accagatgat attatcagaa agatagaaga aagatttaaa
    37621 aagccttggc tagtagaaga gataagtttt aatttagcaa ctagtattgc tgttgtttat
    37681 tacccagaaa atgcagaaac gacagaagat gtaattgttg ctatagattt ctctatacat
    37741 gaagcaaaac gttctgagtc aaggcgagtt gtttatgcag atgcgtctat aagtgaaaag
    37801 ataaagaaga aacatgttat taaaaattgt ttaaaagaag cattaaaaaa tgatgggttt
    37861 gaagtgtact accaacctat attttctaat agcaggggca ggtttacttc agcagaggca
    37921 ttacttaggc tagaacacaa agagttaggt tttatacctc ctgatgaatt tattccaatt
    37981 gcagaacaaa caggattgat taactcaatt ggattagtag tctttgagaa ggtatgtaga
    38041 tttatagcta gtgatgagtt tgaaaattta aaattggata atatagctgt gaatctttct
    38101 gtagtacaat gtatgcaaaa aaacttagct gatgatttac tttatcttat gaataaatat
    38161 aatgtatcac caactaaatt taaacttgaa attactgaga ctgtggctat gggctctttt
    38221 aatataatta aagaaacaat ggaaagatta attgatttag gtgttaagtt tgcacttgat
    38281 gattttggaa ttgcttattc tggagttact aatatgcttt ctttaccatt ttcagtaatt
    38341 aagttagata agagtttgat ttggtctatg aatgaagata gtaggcataa gcttacagtt
    38401 gagacgatta tagctcttat aaataaattg aatatgaagg ctgtagcaga aggtgttgaa
    38461 accattaagt gtgctgaaga tttaatagct atggagtgtg aatatttgca agggtattat
    38521 ttttctaaac cagttccaga agatgttttt aaaaatttgt tgaaagaaaa tacttttaca
    38581 agttaacacc ctttattttt ttaattacaa ctttgttcat tgacactgcc agcgatatat
    38641 tataaaatta acacaataat aaatattaaa tttattatac atattattaa tattaattaa
    38701 tggagtaaag atttattata ttttatttaa ttatcctaaa gtgagaaagg aagatgtgac
    38761 atgaataaaa agaaaatatt agtgccaatt gatgggacag aaagaagtat gcattctttg
    38821 gaatttataa aaggaatatt taaaaaagat gaagttgaag tagaaataat gaatgtaaaa
    38881 gaactagtat ttattgatgg catatccctg gctgaagaga taaaaaattc tgaaaattta
    38941 ggaagaagaa tcttggataa agcagcagag ataatgggag agtatgatgt taaagtacat
    39001 tttacttttg gatatccagg agatgagata attagaaaag caaaagaaga taatgtagat
    39061 tttatagtta tgacaaaatc tactaaaaaa ggtcttacaa gaatgatagg ttcagtaaca
    39121 gcaagtgtag ttaaacaagc aaaatgtata gtgatgattg taccggagta gaattagaaa
    39181 ggtatttgaa atatgaataa aaagatagta gcagtttgtg aaagtgaaag aagtttaaat
    39241 tctcttaaga cagctgcaaa tgatttagga tttgatttag tttatgaaat tcaagaaaat
    39301 gataatataa aaaatgaact ttctatttat gatatagaag atgcatctat agtgttattt
    39361 gttacaagtg attctataga aagcatagag aagattgata gatttataga tagagaattt
    39421 tatgaagttg atccaaagta tataatagag gatgcaaaat ctatagtaag tgaaatttta
    39481 atagacttaa attaatcgtg ggtgtcgcac taagaataga gcgacgcctt tttacatatt
    39541 ttacataaga aataaagggc tgtctcaaac tatagataag actctttttc attgttgaaa
    39601 tgatgaaaat atatttgatt tcatacaaaa aagagtgcct cataactaaa aagttcactt
    39661 tgagacactc catttttata tgttagttgc atttaaggta aaatagaagt ttaatcatgt
    39721 tgaaataact gaattacatc ttctaaatga ttgtacattg atgtataacc taagtaagag
    39781 tctttactat caaatgccat cattatcttg ttatggtggc taatcatatc cttaagagaa
    39841 tttctatatt taaaaccgaa gtattggtta gaagaaactg ttttaggtat taactcataa
    39901 tattttataa aaattggatt atgagaagct aaaattatct ctttgtgaaa ttgtaagtca
    39961 gcatcataac attcttctat caaaccatga gaaaccttat ccaattcctc tattgacttt
    40021 aggtaagtat attttatttt ttgaagttct ttagatgtag catttaaaat tgctagttta
    40081 gtagcttcac tttcgataag ttgtcttgct cttaaagatt ctacataaaa ttcttgtatg
    40141 ttatgatttt tatagatttc accatcatca aataggtcaa cttttttaaa tccttcaact
    40201 ataggacttg agtaaggcac agctctaagt attccttcct tacagagtat atttatggca
    40261 tttcttacag gtattctact tatgttaaat aggtcagcaa tctttctctc acttggtaat
    40321 ttactattaa ttttaaaagt tccatttaga atgtttaatt taatataatc aataattttt
    40381 tcagtggcat ttaagctttt cataaaaact cctaaacata taattttttt atgattgtat
    40441 tatatcatag ttaagagtta aatctacttt tttataatat gtataagaca ggatagaggc
    40501 caaaatagct ataatagcca agataaagta tccatattgt attgaagtat attccgatat
    40561 tttaccaact atcattggaa atacagacat tcctatagca taaattgctt gaaaaaaacc
    40621 cattgcagta gatttctgat gttttggtac atttttcata gactcgctag taaggtaaga
    40681 aaggagtata cctgtagata tacctggtag cacttgtagt acaaatatta tattaatatt
    40741 ttttattgtt ggaactaaaa agcaataggc tgaaactaaa ataaaaacta aaggaatcca
    40801 aaattttggc ccatacttag tacaaaattt agtagaagca aagccagaag aagcaacagc
    40861 acacaccatg taagatatag aagatagacc tatgagggta gaactagaac ctaagttttt
    40921 aagaatttga gtagtaaatg acattgttgt agacatttga ataccctgtt gaattaatgc
    40981 cataaaggaa aaaaatacaa gtcttttatc tttgcagata gataaatatt ttttaattga
    41041 tattttttca ggtatttttt ttgaattatg attttttata tttaaggcta gaatcgttcc
    41101 aataactgaa gacaatacac tcatgataca taaggctgtc attcctaatt tttcataaaa
    41161 taaagtactc ataacaaatc caatcaacat tcctagattg tttaccaata ttatcgtact
    41221 cgtagctttt tgttgatgtt ctgtagaaaa aaagttggta tagtgaacca tgtaagaaat
    41281 ccaagttgat gatgatattc cagataaaat gttagctata agaaaaccat tgcctccagg
    41341 agaaaagact cttataagag atgctaaacc agcaaagaaa attccagaca gtataaatat
    41401 tttatgttta tttatagaat cagcaaatac tccaacagga atccttaata aacattgtga
    41461 gattccatat gcacctatta ttataccaat catatttgta gatacattac atgcacttaa
    41521 atatggattt tggaaaggta tatatacata ttgtgaaaac cagaaaaatg taacaataga
    41581 aagtaaaagt aatttttgat tagatatttt tttcatatta tcctccatat ggtattttta
    41641 taatgcaaat aatataccat ttagattttt taatcaagta ctttctctat taaaaaaaat
    41701 ttaaatatat aattattatt tacaaaaaaa ttatacttta tagttgaatt aagaaaacgc
    41761 ttatgatact ataaagttaa caaatttgaa tatataacag tagacgtgtt actggtaatg
    41821 caggcaagat cgaatcaatt tttatagatt atctatagag tactatgggt ataatctatt
    41881 tatttggttc gatttttttg tttaattggc cataaaacaa aaagcctata gttgaaaatg
    41941 gaggattttt tatggattat aaaaatatag ctcaagaaat tctactaaat gtaggaggaa
    42001 aagagaatgt aaatgaagtg actcattgta tgacaagact tagatttaaa gtcaaaagcg
    42061 cttctaaagt gaataaggat aaattatcta aaactgaagg agtaataact gttgttgaga
    42121 gtatgggtca aatacaagtt gtaattggaa ataaagtaaa aacagtatat gatgaggtta
    42181 taaaaattgt acctcaatca aatgacgtag gtaaaaagaa tgaaagtcaa gaaaagcaaa
    42241 gtataattaa ttctatatta tcagcagtag ctggtatatt tacacctaca atacctgcta
    42301 tagcaggggt tggtatgatt aaaggtattt tatcagttct tgctatgtat tatatgaata
    42361 aaaatggagt agatataaaa gaaacgcaaa gttatattat cttaaatgcc atggccgatt
    42421 ctatattcta ttttatgcct attatactag gatatactgc ggctaaagta tttaatgcaa
    42481 ataaaataat atctatggta ttaggagcta cactttgtta tccaactttt actgctttga
    42541 tggcaggtga agaatctgtt agatttttag gattggcagt aacaaaagct acgtatacat
    42601 catctgttat tccaataatt attgctatat gggctctttc atacgttgaa aaagtattag
    42661 aaaagtatat acctgaggta attaaaataa taatggttcc aactttatca ttggttgtaa
    42721 tgttacctgc aacattattt ttatttggac caatcggaat ttatatagga aatgtcataa
    42781 acttttctta taagtatatt tatgaattaa gcccagcgtt gtgtggagca tttgtaggtg
    42841 ggttatggtg tgtgttggtt atatttggtg ctcatagagc attattacca ataggtataa
    42901 gtgatgttgc tcaaacagga agacagaatt tattggcatt tgcaggagct gcaaactttt
    42961 cacaagcagg ggctgctcta ggagtattct ttaaaactaa aaatcaagga ctaaaaacaa
    43021 tttctatgtc tgcaactata acagctttat ttggtataac tgaacctgca atatacggtg
    43081 ccaatcttag attaaaaaaa cctatggtat gtgccgtaat atgtggtgca tttggaggtg
    43141 cgataatggg aatgggtgga gcttatggta atgcatttgc aaaccaaggt gttttaacta
    43201 tacctgtata tgctgaagca ggtgcattag gtttcttaag ttatctaggt ggttgtgcta
    43261 tagcattttt tggttcagct atttcaactt atctagttgg atttgaagat ttggaagagt
    43321 cttctaataa ggaaaattca tttataaaag tagaaacgaa ggatggtgtt attgatataa
    43381 cttcacctgt agagggcgaa tgtatagaat tgtcagaagt aaaggatgat gtatttgctt
    43441 caaaagctat gggagaaggt atagctgtgt tacctacaaa aggagtaata acagccccta
    43501 ctgattgtga agtagcttct ttatttccaa cattacatgc aataggatta aaattagata
    43561 atggagcaga aatgttaata catgttggaa tcaatacagt agagttaaat ggtaaatact
    43621 ttacaaaaca tgttaatcaa ggagatttag tgaaaaaagg tgataaactt atatcgtttg
    43681 atatagataa aataaaaaag gctggttatg atgtaactac gccagttata gtaaacaata
    43741 catttgattt tgggcaagta gtttcttgta aatctagtta tgtttcaacc aatgataata
    43801 taatttcatt agtattagct ggtaattaag ttaatttgac tagagataag taaattgatt
    43861 taaaaggaaa taagtaaatc aacaattaaa tacatctaaa agtaagttta ttgcaatgaa
    43921 ttgttaaaga aaataattaa tatggagtaa agctattgca atgaagtaac aaagaaaata
    43981 attaatatag agtaagttta ttgtaatgaa ttgttaaaga aaataattaa tatagggtaa
    44041 atctatctca atgaaataac aaagaaaaga gttaatatag agtgaactta ttgcaatgaa
    44101 tcaacaaaaa aaataatcaa tataaaaagg agaaatattt atgaatacag gatttccaaa
    44161 agacttttta tggggagcat catcttctgc atttcaagtg gaaggagctt gggataaaga
    44221 caataaagga aaaaccgtag ccgactataa ttcttttaaa aagtctcatt tacaggcaga
    44281 tactaaagtt gcatcagact tttaccataa ttatgaagaa gatattgaat taatgaaaga
    44341 attagggatg aaaacatatc gtttttcaat ttcatgggca aggataatac cagatggtga
    44401 aggagagata aaccagaaag gtttagactt ttataataag ataatagaca aactaataga
    44461 gtgtgatatt gagccattcg taactttata ccattttgac cttccattta aattagtaga
    44521 gaaatataat ggttgggaaa gtagagaaac tgtctatgct tttgaaagat ttgctaaaat
    44581 atgctttaag cattttggag atagggtaaa gtattggcaa cctcataatg aacagaattt
    44641 aattgttaga gtggaggaga gaattaatat atatgatgaa acagattcat ggaagataga
    44701 taaaatgcgt gctcagatgg attataatct ttgcttagca catgcattag cagtcaatgc
    44761 atgtcatgag atgataaaag aaagtaaaat tggagcagca gtatcatcat ctgtaacata
    44821 tcctcttact tctaagccag aagatgtata tgcagctaga atgaatgata attttaaagt
    44881 gtactatatg cttgatatgc atcattatgg agaatatcca gggtattata tgaagtatct
    44941 tgaaaaaaga aatacagttc ctcacatgga agatggggat aaagagatac ttaaaaaagc
    45001 taaaatggat tttatagctg taaattatta taggacaaat tgtgcagaag ctttacctga
    45061 agattcacaa catccatttg gattaagaga aggaactgtt gattttagta tgtatggact
    45121 atttaaaatg tcaatgaatc ctaacttaga ggcttctgag tatggagcag caatagaccc
    45181 atcaggtctt agagttgcct taaatgaata ttggcaaaga tatcacttac ctgttataat
    45241 aacagaaaat ggtctaggag ctaaagatat attagaagat ggaaaaattc atgatgacta
    45301 tcgtatagac tatttaagaa gtcatataaa tgcatgtaaa ctagctattg aagatggtgt
    45361 agagatgatt ggatattgtc catggtcatt tacagacctt ttaagttctt ctcaaggttt
    45421 taataagcgt tatggacttg tttatataaa tagaactgac catgaagttt tagatttaaa
    45481 aagaatcaag aaagatagtt tttactggta taaagaagtt atagagaaca atggaatagt
    45541 aaaataaaca taaatatata ttgagggttt tagtatgtac attacaaagg tattaaataa
    45601 ttcgttgcta cttgcaaagg atgataatga tgaagaaata atattgatgg ggaaaggcat
    45661 tggccataat tataaggccg ggtctgagtt aaataaaaaa gatatagaaa agatttttgt
    45721 tcttcatgat gaaaatataa aaaagagtat tatacaattg gcaacagaaa tagatgcaga
    45781 atattttggt atagctcaaa tgataattgc atatggaata gaaaaatata acttaaaact
    45841 tatgaaccat atatatttgg ccttaactga ccacattgcc tttgcagtga gacgctttaa
    45901 agaaggtata atgattgaaa atcactattt gttcgaaatc aaggaattta atccaaaaga
    45961 gtatgatata ggaaaatatg ctattactgt ttttaaggag gttcttggtt tagacctacc
    46021 agaagaagag attggtaata tagcaggcca ttttataaat gcacaacaag ataatcctta
    46081 tagtgataga aataagagaa gtgctaaaat tgtaaatgct attttgcaaa tagttcatta
    46141 tcacttttct attgtatata ataaagagtc attttattat aggagatttg taatgcatct
    46201 aaaggccttt tcacaaagat ttctggctaa tgaaccatca agagaaaaaa tagattttat
    46261 atatgagcaa gttcaaaaaa attgtaagga agagtatgaa tgtgtgaaaa aggtaggagc
    46321 ttatatgtat aaggaatact ccagagaatt accaagacaa gaggagttat atttgatgat
    46381 tcatattcat aagattttag gagaattatg cgaaaatagt tagaatatct taatatgcta
    46441 gtaatattat aaaaaattaa aaattaacta cactttaaat atgaacaatt gcattactta
    46501 agggggttgt cttaaaattg atttttttaa ttaaatttga agctcaactc cttttttaaa
    46561 cctttatatc attacaactt taatgtttat acgcctgtac tttagatgta tatctacttt
    46621 ttacatttct caacatgcaa acctaccatt ttttgcagta tgttaattga aataaaccat
    46681 tgagttaacc ctaaatttat tctggactta taggaatagt tccaaagata ctttaaaata
    46741 ttttctggaa taattgctta tttcacatat gttttattgt gatgttttga tttaataaat
    46801 tcttcaactc tgtatatagt aaatgtatta tcatgtttgt tttattcaag cacacttata
    46861 atacgcttga ttttttcaca atgatgaaat atactaatgg gtgttatagg tataagaatc
    46921 gaattacact tgaagaagta agtgataaat gagatataaa tcctcatatg ataaactact
    46981 atttcattga ggaacatatt ctaagtgatg tcaagatact acaatatgac taaattccta
    47041 gtgataaaaa agtctacgaa tgagtgtaga aaggggaact aagatgaata aagttttaat
    47101 tatagatgat gataaagatc tttgtatttt gttaaaaaag agtatttcaa ttgaacagat
    47161 tgaagcaaac tattgccatt ctgggagaga tggactggtt gaactttcaa aacaagatta
    47221 tcagttagta gttctagacg tgatgatgcc aggtatggat ggttttgaaa ctatggaaca
    47281 aatacgaaaa tatagcagta ttcccatctt aatgctgaca tcaaaaagcg ataattacag
    47341 caaagtgcat ggattgcgag ctggtgcaga tgactatcta accaaaccat ttgaaattac
    47401 agaatttact gctcgtgtac tgtctttaat ccgacgttac acccgtttta atccaaaaaa
    47461 tgaggaacca cagcaacttt ctttcaaagg tttgttgatt gaccttgacc agcatacagt
    47521 gactaccaat ggcgaaccta tgttgcttcc acccaaggaa tttggtgtgc ttctttactg
    47581 tgcaaggcat cagggtaaga ttttgacaaa acagcagatt tatgaagctg tgtggggtga
    47641 gccatatgta tatgatgaca gcaatatcat ggctattatc agtcgcctaa gaaaaaaaat
    47701 tgaactagac ccatcatctc ccatctattt gcaaacaata aaaggaattg ggtatcaatt
    47761 caacacggag gtatgaaaat ggatgaacaa acgataattc ttttcttatt cttgctgtcg
    47821 attggtctac tttttctttc catatttgct ttttccaagc ttgggcatat tcacaaacgc
    47881 ctaaaagata tagaggagat actggctgat gtacaaattg gaaacgaaaa tcgcaaaata
    47941 ctaattaagc catgtgatgt tatggctcca ttggtttatc agcttaatga gattgtctac
    48001 gattatgaaa ataagttact ttctctgaaa aagtcggata aagctagcaa acaacttatg
    48061 acaagtctat ctcatgatgt tcgtacacca ttaacaactc tgattggtta tctggatgct
    48121 gtacacagtg gtatagttat cggacaagaa cgggaggaat accttgaaat cgctaggcgt
    48181 agagcctacg acctcaagga ttacattgat gttctttttg actggtttcg gttgaactct
    48241 gatgaattta cgctgtcaat agaatctgtg gaaattgcag agttgtcacg caatattttg
    48301 aaagactgga ttcctatatt ccatgaaaag aaactgaatt ttgaaattga tattccagaa
    48361 aatcgactaa tggtaaatct cgacccagat ggatattccc gtgtggttaa taatctggtt
    48421 caaaatgttc ttgctcacag taaggctcgg caaatcaaga ttaccatgag tgaggattcc
    48481 agaatggtat tactccgtgt tgatgatgat ggtgttggga ttgccagaga aaatttacca
    48541 catatttttg agcgactgta taagtgtgat aaaggacgtt cagaaaaagg aagtggtctt
    48601 ggtctatcta ttgtcagtca aatggtggag cgaatgggtg gtcagatttc cgtagaaagt
    48661 gagattggaa agtataccgt atttacagtg agcttaccat taatataaaa ttgtttttcc
    48721 cccatgtgac atgtatacac agggggattt tttgtaagtt ataaagttgc aaggttaata
    48781 caaggttgtt gcaaggttaa tgtaagattg aggtggtatg atagctttaa taaacaaagg
    48841 aggattttat tatttatgaa taatttaatt attgaaacaa aaaatctcac taagcaatat
    48901 ggtgctcaaa agagcgtttc taatttgaat attcacgttc agaaaggtcg catttacgga
    48961 ttgctaggaa gaaacggtgc gggtaagacg acaactatga aaatgttatt gggtctaaca
    49021 aaaccaacta ctggagaaat taaaatattt ggtcaggaca ttcacaataa cgagaagaag
    49081 cttcttcctc gaattggatg tttgattgaa tcaccagggt tttatcctaa tctgacagga
    49141 acagaaaatc tcaaaatctt tgcaaggctt agaggaatac caaaaaaaaa tgcagtagaa
    49201 tcagcactgg atgtagtcgg tttgccaaac aatgacaaaa agttattttc tcaatactct
    49261 cttggtatga aacagaggtt ggcgattgct cttgccatta tgcatgaccc acaactgttg
    49321 attttggacg agcctatcaa tggacttgat cctattggta ttgcagaagt aagaaagttt
    49381 attcgtgatc tctgcacaga acgaggtaaa actattttga tttccagtca cattctttct
    49441 gaaattgcct tgttggcaga tgacattggt attattgacc atggtatttt gttggaggaa
    49501 gaaagtcttg cccaattaga agaaaaaaac agtaaataca tacattttgc tgtatctgat
    49561 gcagcacatg cagcaaggct tctggaaacc caacttaacc tgaaaaattt caaagtagca
    49621 gataattaca atattatgct atatgataca gatgtttctg ttgcagaaat aaatcgtgct
    49681 ttcattctaa acaacattga ggtatcagaa tctcatttat gcgaagatac tcttgaagat
    49741 tacttcaaga aagtaacagg gggtgaagga attgcttgat ataatttcat gtgaattttc
    49801 aaaactcaag cggttaaaat ttatactgat ttctatttta ggtgcttgct tatttccaat
    49861 tcccgcaaca attttgattg caaaggacaa cttgcccttt gagcaattgt tcaaattagt
    49921 tgtcaatttt ggatattttt tgctattacc aattgtatta agtattgttg ctagccaact
    49981 attttttata gaaagagata acgatgtatt aaaaaatctt gcaacagtgc cagtgccaaa
    50041 aggaaaactg gctctggcaa aattggctgt gttgctgttt atatcattgt tctactccgt
    50101 tgcagggtta ggagctacta tcattggagg tttcattgtt ggaatagtag agggggttac
    50161 tgtaaaatta ggaatgagca ttgcttttgg aattatgttt tttgttactg tacttcctgt
    50221 tgtggtactc attgtatatt tcaatcgcag ctatattttt tctattatac tttcttttgt
    50281 atatgctatt ttcaatttta gtatatcctt aaatattatc aattttgaac caaataaccc
    50341 actattaagc gtattacctg ctcctgttat aatgcgttgg tggatggcat tttggggaga
    50401 tcccactggc gaatacacgg cacttagaca gccatatttg ctctcaactc ctgcttgtgt
    50461 aggtatttta tgtttgataa ccattatagc agtcctgctg atttgtatga tatacaaaaa
    50521 gcaggagaat taggaggtat ctatgcttga tataattact acagaatttc aaaaaataaa
    50581 aagatataat attctatgga ttggtgttgt tgctgttctg ttttcagcac tattggcagt
    50641 ttttcaggtt gtaagttcac acggcagtga ccctctaacg tatgagagct ttgcaaacgg
    50701 agttatctgg aataatttca gtcttagttt tccatttggg attacactta ttggaggata
    50761 tcttattaac cgtgaatata cagaccaaac cttaaaaaac atcctaactg ttcctatttc
    50821 attacgcaaa ttgctagtgg gaaaattaat tgcggttgga ggaattgctg tattgttaag
    50881 tcttttcagc ttcttatgca catttatttt ggggttgtta ttttgcaata tcaatatatc
    50941 tgttacactc attgtaaaat ccttagttca gattttgtct gttaatctgt attgttatgt
    51001 tgctgttttg ccgattattg cttattttgg tcgtaaacaa aatgcatttc tgacgggtgt
    51061 agggattgca tttgtatacg ggttttgtgg tgtgtttgtt gctggaagaa atctgacaga
    51121 cttttatcca atcacagcag gtcttggtct agttggatac aacaatggtg ctggtactgt
    51181 ttatcagcca cttatcggtg ctatgacttt gattgtaatt ctaatcttga caactattct
    51241 acttgtattt acacctaatt atgataaagt tatgaccata tctaagaaaa aagggataaa
    51301 gcataaaaag tattctcata aataacataa atattgtttc tattatttaa gttgctagac
    51361 agtgaaaaga aaaaatctgt cgtacagtgg ctatatgctc atgtattcaa gaagctacag
    51421 ttttgatttt tcataactgt agcttctttt tgtatttatt aaatcacggc tatcataata
    51481 cttgcaatag gtatagcgac aagagagagt actgtcatgt gtgttttata tattacatta
    51541 agtatattct aaaatatatg ttttaaataa tatatttgaa aataggaata tttaaataaa
    51601 aaaaataata ttatatatag ttgacaaaaa ttggcaaatg atataatata taaatataga
    51661 aatagtaatc attactatta ttaaaaaggg aggaattaat tatgaactta aaaggaacta
    51721 aaacagaaaa gaatttaatg gcaggatttg caggagagtc agaagcaaga aataaatata
    51781 catactacgc ttcaaaagca agaaaagaag gttataatca aatagctgct atatttgaag
    51841 aaactgctaa caatgaaaag gaacatgcta aattatggtt taaactttta catgatggta
    51901 tgccttcaac agaggaaaat ttaaaggatg cagcagcagg tgaaaactat gaatggactg
    51961 atatgtatgc aaaatttgct aaagaagcta gagaagaagg atttgacaag atagcttatt
    52021 tatttgaagc tgttggaaaa atagagaaag agcatgaaga aagatactta aagcttttag
    52081 aaaacttaaa tgaaggaaaa atatttaaaa gagatgaaga agttgtttgg caatgtcaaa
    52141 actgtggtca tgtgtatgta ggtactgaag caccagaaaa atgcccagta tgtgaccatc
    52201 caaaagcgta tttcaatata aaagctgaaa attattaaaa attatcatta ttaaaaagga
    52261 atgttaacta tgaaattttc taaacaacga gaactgattt taaatgaaat attaaataat
    52321 ccggttcatc ctactgcgga ttacctatat gaaaacttaa aaaaagataa tccaaattta
    52381 agcttaggaa ctgtttatag aaatttagct cagctaacgg aacatggctt tataagaaag
    52441 gttagtattc cagggtatcc agatagattt gatggtcgaa tagataatca ctatcatatc
    52501 atatgtgaag tatgtggaga ggtatacgat ttagaatccg aagttcttaa taatttacaa
    52561 gaattaatat ctgaggagac agacataaaa ataacatctt ataacataag ttttaaaggg
    52621 atttgtaata attgtaaaag gtgtagccaa gtaggttagt tttcaaattc taaaaataat
    52681 taatgaggtg agtttatgtg tagtgaacaa aaatttttta tatgtgagac ttgtggaaac
    52741 cttgtaggaa tgatacaaag tggtggtgta cctattttct gttgtggaaa acctatgaag
    52801 gaacttgttc ctaatacaac agatgcagca gttgaaaaac atgtacctgt aattgaagtg
    52861 gatggaaata atgttactgt taaagtaagt agcacaactc atccaatgac taaagaacat
    52921 catatagcat gggtgtatct tatgactgaa caaggtagtc aacgcaaatg tctagcagta
    52981 gatggagaac ctgtagttaa atttgctttg aatgatgatg ataaagtgat ttctgcctat
    53041 gcttactgta atttacatgg attatggaag gctgaactgt aatatttaaa taaattgagt
    53101 caaagaggag gctataaatg tattttcata caaatagcct cctataaatt tatataaatt
    53161 caagttttaa ttttgtttaa tgtcaatata atataaggag aaaataatat gtcaatttat
    53221 aaatgtagtg tttgtggata tatttatgat gaatctaaga atgataaaac atgggatgaa
    53281 ttaagtgaag attgggaatg ccctgtatgt acaaaaggtc gtagttattt tggaaagata
    53341 agcactgttt actatgaaga agatgaaaag atagcagaag acatagttga aaatgaatct
    53401 aaattaaata ctgaaaaaga gggagattta aattacttaa gtacatattt aagaagagat
    53461 gatgaagttg agaaacatat ggatataatt cacgaaatgg ctgttacagg aaaatctata
    53521 atagaaccta tgagaactaa actaccagta atttcttggg atgatatttt aattatgggt
    53581 gctcaattaa atccattacc tttaaatgag catgatgaag taaatacaac aacaataata
    53641 ggaaaaaaag caaagaaacc tatgattata gaaaatccag tttatatttc ccacatgtca
    53701 tttggagctt tgtctaaaga actaaaaata gctttagcaa agggtgcagc ccaaaataag
    53761 acagctatgt gtagtggtga aggaggtata ttaccagaag agaaggaagc ttcatacaag
    53821 tatatttttg agtatgtacc aaataaatat agtgtaactg aagaaaatct taaaaattct
    53881 gatgctatag agataaaaat tggtcaagga acaaaacctg gtatgggagg tcatcttcct
    53941 ggcgaaaaag taactgaaga aatagcaaaa gtaagaaata agcctgtagg tcaagatgta
    54001 ataagtccat cttgttttga ggaaatacaa tccaaagagg atttaaagaa acttatagat
    54061 gaacttagag aagtttcaga agggcgtcca ataggtgtta aaatttcagc aggtcatatt
    54121 gaaaaagata tggaatttat agcttatgct aaacctgatt ttgtaactat tgatggtcgt
    54181 ggtggagcta ctggagcaag tccaaagttg cttaaagatg caacttcaat tccaacaata
    54241 tttgctcttt atcgtgctag aaaatacata gatacacatg gattagatat agatttggtt
    54301 ataacaggtg ggcttcgtat atctactgat tttgctaagg caattgctat gggggctgat
    54361 gcagttgcta ttgcaagttc tgctcttatg gcagcggcgt gtcaacaata tcgtatatgt
    54421 ggttctggta agtgtcctgt aggagtagct actcaggatg aggaattgag aaaaagatta
    54481 catatagaaa atagtgctaa tagagtggca aactttttaa atgtaagttt agaagaactt
    54541 aagacttttg ctagaatttc tggtcataaa gatatacatg atttaagtgt tgatgattta
    54601 tatactgtta actctgaaat atcaaattat acaaacatac agcatgtata gattgaagtt
    54661 tgaaactatt agattttaga tagctataaa agtaaatgat aaatatattt tataaaagag
    54721 agtgtcttaa aatgaacttt ttagttcata agtcactctt ttttgtatga agccaaatat
    54781 atttatcatt ttagcaatga aaaagaatct tatctcttta ttttgagata gactccttct
    54841 atttatgaaa attaaagata ttaaattgta aaatattctg tgatgtcgta ctattaaact
    54901 aaagattctg tactttaaag taaataaaaa tttaaggagt gcttattatg gaaagattga
    54961 agatacaagt tttagttgac aataatactt atattgatag atactttgtt ggagaaccag
    55021 cagtaagcta ttatattgag attgatggaa ataggatttt gtttgattcg ggatattcag
    55081 atgtattcat atcaaacgct gaaaaattaa atatagattt aggaaattta actcatgttg
    55141 ttttttctca tggacataat gaccatacaa gaggtatcca gtttttagaa aataggtatg
    55201 atttatctac agttgaactt atttcacatc ctaactgttt tatacctaga aaacatggag
    55261 agaaaagcat aggagctcca ttttctgctg aggaaattaa aaatattttt atgtataatc
    55321 ctaaggataa gccatttaat ctaagtaaga attgtgtgtt tttaggtgaa ataccttcta
    55381 taaataattt tgaaaaaaga gctaaaattg gtaaatgtaa aataggagat ttgtgggaag
    55441 atgattatgt tttagatgat tctgctatag tttgtaaaac tgataaaggt atttttattg
    55501 ttacaggatg ttctcatagt ggaatttgta atatagtaga gtatgctaaa agggtatgtg
    55561 gagatgatag agttattggt atactaggtg gttttcatct ttttgaattg aataataggt
    55621 tagatagtac aatacaatat ttggataaag aagaaataag aatgttgtac ccttgtcact
    55681 gtgtttcttt taaggtaaaa gctaagatga atgaaatatt gaatataaat gaagttggag
    55741 tagggctgac cataagtata tagtatttga aagtatataa gatttaagtt gaaggttaaa
    55801 aagatatttt ctatataaag tatctttttt gttacgttta ttttaagaga aatcaatata
    55861 cttttatatg aaatattgta aaaaaattta tttttaatga gaatatattt t
//
LOCUS       DAMJVX010000010        53078 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMJVX010000010 DAMJVX010000000
VERSION     DAMJVX010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53078
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.011"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(355..3462)
                     /locus_tag="PC407_000534"
     CDS             complement(355..3462)
                     /locus_tag="PC407_000534"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDF2496014.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(3555..3787)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3962..4489)
                     /locus_tag="PC407_000535"
     CDS             complement(3962..4489)
                     /locus_tag="PC407_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDF2496015.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(4524..5309)
                     /locus_tag="PC407_000536"
     CDS             complement(4524..5309)
                     /locus_tag="PC407_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDF2496016.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(5319..5576)
                     /locus_tag="PC407_000537"
     CDS             complement(5319..5576)
                     /locus_tag="PC407_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDF2496017.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFNILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(5595..6053)
                     /locus_tag="PC407_000538"
     CDS             complement(5595..6053)
                     /locus_tag="PC407_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDF2496018.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(6093..6797)
                     /locus_tag="PC407_000539"
     CDS             complement(6093..6797)
                     /locus_tag="PC407_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDF2496019.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(6807..7442)
                     /locus_tag="PC407_000540"
     CDS             complement(6807..7442)
                     /locus_tag="PC407_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496020.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IPVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            7823..8863
                     /locus_tag="PC407_000541"
     CDS             7823..8863
                     /locus_tag="PC407_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2496021.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGIITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(9326..10681)
                     /locus_tag="PC407_000542"
     CDS             complement(9326..10681)
                     /locus_tag="PC407_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HDF2496022.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPISTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(10707..10976)
                     /locus_tag="PC407_000543"
     CDS             complement(10707..10976)
                     /locus_tag="PC407_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2496023.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(11266..13206)
                     /locus_tag="PC407_000544"
     CDS             complement(11266..13206)
                     /locus_tag="PC407_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496024.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCNMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAKDSGYNIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNQLDIDVEWDFTDYYEKLEKGENFDITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKTIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNSDEKQPNTPKDD
                     VESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNPITENDDNYDLSSKSVFGTNEH
                     SFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCILTPIMI
                     GSILIVNPDTRKSIIKFIKLKK"
     gene            complement(13390..14865)
                     /gene="spoIVA"
                     /locus_tag="PC407_000545"
     CDS             complement(13390..14865)
                     /gene="spoIVA"
                     /locus_tag="PC407_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HDF2496025.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(15037..16332)
                     /locus_tag="PC407_000546"
     CDS             complement(15037..16332)
                     /locus_tag="PC407_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2496026.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNKSNENTTKVS"
     gene            complement(16516..17925)
                     /locus_tag="PC407_000547"
     CDS             complement(16516..17925)
                     /locus_tag="PC407_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2496027.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            complement(17977..19116)
                     /locus_tag="PC407_000548"
     CDS             complement(17977..19116)
                     /locus_tag="PC407_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2496028.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            19435..20085
                     /locus_tag="PC407_000549"
     CDS             19435..20085
                     /locus_tag="PC407_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2496029.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSGDDELTMKMISSIEYIIKHAK"
     gene            complement(20242..21261)
                     /locus_tag="PC407_000550"
     CDS             complement(20242..21261)
                     /locus_tag="PC407_000550"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2496030.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVINKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(21292..21906)
                     /gene="plsY"
                     /locus_tag="PC407_000551"
     CDS             complement(21292..21906)
                     /gene="plsY"
                     /locus_tag="PC407_000551"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDF2496031.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(21941..23260)
                     /gene="der"
                     /locus_tag="PC407_000552"
     CDS             complement(21941..23260)
                     /gene="der"
                     /locus_tag="PC407_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDF2496032.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(23294..24622)
                     /locus_tag="PC407_000553"
     CDS             complement(23294..24622)
                     /locus_tag="PC407_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HDF2496033.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEIGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPENILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(24860..25060)
                     /locus_tag="PC407_000554"
     CDS             complement(24860..25060)
                     /locus_tag="PC407_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496034.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(25062..25994)
                     /locus_tag="PC407_000555"
     CDS             complement(25062..25994)
                     /locus_tag="PC407_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2496035.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNM
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(26039..26971)
                     /locus_tag="PC407_000556"
     CDS             complement(26039..26971)
                     /locus_tag="PC407_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2496036.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYDLSFFHCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNDVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(27026..28315)
                     /locus_tag="PC407_000557"
     CDS             complement(27026..28315)
                     /locus_tag="PC407_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2496037.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(28344..29036)
                     /locus_tag="PC407_000558"
     CDS             complement(28344..29036)
                     /locus_tag="PC407_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2496038.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(29051..29812)
                     /gene="pgeF"
                     /locus_tag="PC407_000559"
     CDS             complement(29051..29812)
                     /gene="pgeF"
                     /locus_tag="PC407_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDF2496039.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENITSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(29809..30270)
                     /gene="nrdR"
                     /locus_tag="PC407_000560"
     CDS             complement(29809..30270)
                     /gene="nrdR"
                     /locus_tag="PC407_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDF2496040.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIEEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(30292..30555)
                     /locus_tag="PC407_000561"
     CDS             complement(30292..30555)
                     /locus_tag="PC407_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2496041.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(30659..31432)
                     /gene="sigG"
                     /locus_tag="PC407_000562"
     CDS             complement(30659..31432)
                     /gene="sigG"
                     /locus_tag="PC407_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HDF2496042.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(31524..32246)
                     /gene="sigE"
                     /locus_tag="PC407_000563"
     CDS             complement(31524..32246)
                     /gene="sigE"
                     /locus_tag="PC407_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HDF2496043.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(32271..33143)
                     /locus_tag="PC407_000564"
     CDS             complement(32271..33143)
                     /locus_tag="PC407_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HDF2496044.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGSAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(33434..34708)
                     /locus_tag="PC407_000565"
     CDS             complement(33434..34708)
                     /locus_tag="PC407_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2496045.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(34992..36149)
                     /gene="ftsZ"
                     /locus_tag="PC407_000566"
     CDS             complement(34992..36149)
                     /gene="ftsZ"
                     /locus_tag="PC407_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDF2496046.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(36339..36692)
                     /locus_tag="PC407_000567"
     CDS             complement(36339..36692)
                     /locus_tag="PC407_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HDF2496047.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(36791..37465)
                     /locus_tag="PC407_000568"
     CDS             complement(36791..37465)
                     /locus_tag="PC407_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2496048.1"
                     /translation="MKKKIVILGAFILGIFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(37470..38168)
                     /locus_tag="PC407_000569"
     CDS             complement(37470..38168)
                     /locus_tag="PC407_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2496049.1"
                     /translation="MKKRFINKSIIIGSVVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKTEKENLQSEVNSVKKDIETYRGKNNKGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVFNTYMY
                     LKEDKLCMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(38191..38931)
                     /locus_tag="PC407_000570"
     CDS             complement(38191..38931)
                     /locus_tag="PC407_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HDF2496050.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNINLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSVDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(39201..40427)
                     /gene="murG"
                     /locus_tag="PC407_000571"
     CDS             complement(39201..40427)
                     /gene="murG"
                     /locus_tag="PC407_000571"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2496051.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(40445..41575)
                     /gene="spoVE"
                     /locus_tag="PC407_000572"
     CDS             complement(40445..41575)
                     /gene="spoVE"
                     /locus_tag="PC407_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HDF2496052.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(41627..42982)
                     /locus_tag="PC407_000573"
     CDS             complement(41627..42982)
                     /locus_tag="PC407_000573"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDF2496053.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEVVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(43001..43969)
                     /locus_tag="PC407_000574"
     CDS             complement(43001..43969)
                     /locus_tag="PC407_000574"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDF2496054.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(44010..45383)
                     /locus_tag="PC407_000575"
     CDS             complement(44010..45383)
                     /locus_tag="PC407_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDF2496055.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREASAQNAFVA
                     IVGENLDGHTFMKPAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDIYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(45550..47529)
                     /locus_tag="PC407_000576"
     CDS             complement(45550..47529)
                     /locus_tag="PC407_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HDF2496056.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(47541..47888)
                     /locus_tag="PC407_000577"
     CDS             complement(47541..47888)
                     /locus_tag="PC407_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496057.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIAIFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(48374..49309)
                     /gene="rsmH"
                     /locus_tag="PC407_000578"
     CDS             complement(48374..49309)
                     /gene="rsmH"
                     /locus_tag="PC407_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDF2496058.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGKLVDIIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNKGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(49339..50085)
                     /locus_tag="PC407_000579"
     CDS             complement(49339..50085)
                     /locus_tag="PC407_000579"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDF2496059.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKDDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKHNISEE"
     gene            complement(50482..51768)
                     /locus_tag="PC407_000580"
     CDS             complement(50482..51768)
                     /locus_tag="PC407_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2496060.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(51783..53048)
                     /locus_tag="PC407_000581"
     CDS             complement(51783..53048)
                     /locus_tag="PC407_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2496061.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
BASE COUNT        16349 a         9823 c         5540 g        21366 t
ORIGIN      
        1 tcacgagtgt gcgtagctat tttttaatca atacttaact ttttgttttc accatacatc
       61 aaaacgcacc cctttgcata ataaataaac caccctgcag aacacacgat aaatttataa
      121 ataaatttat cagaagtagc ttactaaaaa tattattcat cctcacttaa acgtttattc
      181 attaatattc tatcacatga cataaataat ttatatgatt tatatagatt tatttattgt
      241 aaattttgga atattaaaac attttattga atagataatt atattacata aaaaatccct
      301 atagaaatct ataaactaat ttctataggg attttggtta actaactatt ctttttattt
      361 ttttataact tttatacctg tcatatggtc atttaagttt tgttcctcta aagatttatc
      421 atctactctg tttatttcta aagctaaagt ttcagacttg atatattcat taaaatggtc
      481 aacagcctta gcaatttcat catcaccaca gtattctatt actatattat ctaatacttc
      541 aaagttatta gattttctta attgctgtac tttagatata aattctctag cataaccttc
      601 attaatcaac tcttcagtta gcttagtatc tagtataaca aaaagattgt tttcaacaga
      661 tacattaaat ccttccttag cagttatacc tattaatact aaatccttat taaattcaaa
      721 agcttcgcca tctaaatcaa ctgttaaagt ctcacctttt tcaagtttat tagcagtttc
      781 atgagcattt aatttactta atgctccaac gaataaattc atcttagaac ctaataatgg
      841 ccctgcttcc ttaaagtttg gttttaaact aaagttcata tactcatcta aatccttagc
      901 aaatattacc tctttaacat taagctcttc ttttattaaa tcaactacat cacttattgt
      961 atcttcaaac ttaccatcta ctaacacttt ttgtaatggt tgacgaactt ttattctttc
     1021 tacttctctt gaagctctac ctaaagtaac taagttttta gctaaatcca ttttttcttc
     1081 taatttagta tctactaact cttcattaca tttaggatat tcagctaaat gaacagatac
     1141 ttctcctgtt aagtttctat acatttcttc tgacatatat ggtgcaaatg gagctatagc
     1201 tctacaaaga ccatgtaaca attcataagt tgtattgtac actgcttttt tatcttctgt
     1261 taattcagtt gcccagaatc ttcttcttga acgtcttata taccagttag ataaatcatc
     1321 atttataaaa tcttgtatca tccttaaagt tttatttaac tcaaatatag ctaaattttc
     1381 ttctacatct ttcattaaat tattgaactt agataatatc catctatcaa gttctggtct
     1441 atctttatat tctacaaaaa attcagttgg atttatatca tctgtgtttg catataaagt
     1501 aaagaagtta tatacattct tcatagtacc tataaattta ctttggattt cttttaatcc
     1561 atccatatca aatttagttg gagtccatgg tggagaaaca tatagtaaat accatcttaa
     1621 tgcatctgct ccatattggt caaacaattc cattggattt actgtatttc ctcttgattt
     1681 actcattttc ttaccatctt tatcaagtac aaggtctgga actaaaacac tcttatatgg
     1741 tgcttttcct aatacaaatg ttgatactgc aagtaatgag tagaaccaac ctctagtctg
     1801 gtctatacct tctgatatat agtcagctgg gaacaattgg tcaaaatttt ccttattttc
     1861 aaatggatag tggtgttgag caaatggcat agccccacta tcaaaccaac agtctataac
     1921 ctctgttact cttgtcattg gttttccaca tttttcacat tttaagtgta tatcatctac
     1981 atatggtcta tgaagttcaa ctgtttctgg atttacatct tctatagctt tttcagctaa
     2041 ttcagctctt gaaccaactg aatctgtatg accacattca catctccata taggaagtgg
     2101 agttccccaa tatctagttc ttgatattgc ccagtcattt agattttcta accagtttcc
     2161 aaatctcttc tctcctacaa aacttgggaa ccattttaca ccattattat tttctattaa
     2221 tttatcttta agtttagtca tctctatata ccagcttggt tttgcatagt atactaatgg
     2281 agtttgacat ctccagcagt gtgggtaatt atgaactact ttttctttgc tatataattt
     2341 attttcagaa gctagccatt ttattatttc aacatctagc ccatcttcca ttacaaatct
     2401 accttcccaa ggagtagatg taaatttacc agtttcatct actggttgaa taactggtaa
     2461 atcatatctt ttacctgtgt tgtagtcatc ttcaccaaat gctggtgctg tatgaactat
     2521 accagttcca tcttcagtag ttacataatc tcctactgta ataaaaaatg cttttttgtc
     2581 aacttctaca aaaggcatta attgttcata ttctaaacct tctaggtctt tacctttcat
     2641 ttcttctaaa acttcataat cttcacctag tactttatca gctaaaggtt tagatacata
     2701 atatacctca tcaccttttt taacttttaa ataatctaca tcagcattta ctgttaatga
     2761 aacatttgat ggtaaagtcc atggagttgt tgtccatgct aagaagtatt catcagcatc
     2821 ttttttctta aatttagcta taactgtatt agtttttact tctttatatc cttgtgcaac
     2881 ctcatgacta gcaagaccag ttccacatct tgggcaataa ggaagtattt tatgcccttc
     2941 atatatatat ccttctttgt tgaatttgtt tagtatccac catacagact ctatatagtt
     3001 attatctaaa gttatatatg gattatctaa atcaacttca taagccattc tctcagtcat
     3061 tattctccat tgtttttcaa atgagaaaac tgactctcta catttttggt taaattcagc
     3121 tattccatat tcttctattt gttgcttgct agtaagacct aactctttct ctacttgtat
     3181 ttcaactggt aatccatgtg tatcccaacc agcttttctc tttacttgat aaccagacat
     3241 tgttttatat ctacaaactg aatctttaag cgttctagat aaaacatggt gtactccagg
     3301 atttccatta gctgttggag gtccttcata gaatacgaaa cttgggtcat ctttcccttt
     3361 ttctaaagtt ttttcaagta tatttatgtc tttccaataa tcaaaaactt gtgcttctgc
     3421 ttgttttaca gaactgtcta ccaatggctt aaatttcgcc atgtaaatca ccctttccta
     3481 ctaaatatta taattccaaa attttttatt ctttcacata aatttaaata ctaaaaaact
     3541 cgcccccttt aaaccaaagg gacgagttat aaatcgcgtt accaccctaa ttctatatgt
     3601 aaactaaata ctagctacat ataacactct aatagtataa cggtactaac cggcacagct
     3661 tagtagaatt taatcgttca gcctgcagct caagagtgat ttttatcttt ataaattcat
     3721 tggttctcag catacccaat tctctgtaaa attatctaaa gataaatgtc tctatcaacg
     3781 cttttaaaaa tttatctgtg tttatatgaa attttataac aaataatttg aatagtcaat
     3841 atatttttga aaaatataat aatttctatt tttcttacat ttttatccta tttttaatct
     3901 ttgttataga tttctcaaac tcacacagtt attctacata aaaaataaac aaattacaag
     3961 cttattctaa agttgtagca gcttcatcat tactataatt atacactgtt gttccttcaa
     4021 atgcatttac acatttttca tctacatcat aaaaaatctc atcaacactt cttatttcat
     4081 cttctaacag tgatttaaat ttatttctaa atatcttaaa ttctttgact aaagcatcat
     4141 actcttttct tatctctatg acacggttat tagcttgttc tataatttgt ctagctttta
     4201 aatcagcttc ttcaacaatt atcttagcct ttttattagc cgcgctacaa gtgtcttctg
     4261 ctgcactttg agcagttatt aaagtagctt ttagtgtttc ttcaatattt tcatacttgt
     4321 ttacttggtc ttgatataac cctaattttt ctttaagagc tgtattttcc ctacatagag
     4381 attcataatc ttccttgatg atatctaaaa attcatcaac ctcttcatct ctataccctc
     4441 ttaaaccttt tttaaactct ttattttcta tctcaattgg agttagcatt acatttcctc
     4501 ccagctataa tttaaattag tccttataca attaatcttg cttgaacttt tatttttcct
     4561 tttttggtaa tatctcctat ttgtgttacc ttagctcttc ctttgcctct aatagaaatc
     4621 aaatcatcat tttttactat tttagaagtt gaagttatct tttcataatt gacatggact
     4681 ttttctccat ttatgtattt tgcactatct tgtctagata tattgtaaat accacttatc
     4741 acacaatcta acctatctga agatactgta aatgatactt ctttatattt tgaagaacta
     4801 ttaacaatat tttctctgga aatttccttt acaattacat tatttcttga aaccttttct
     4861 aagtttacaa ttataaaatc gcaaatatca ctacttacaa ctacttgaca aaagctatca
     4921 tgtataatta tatctccaat tttttctctt tttataccta gacccagtat tgaccctaag
     4981 taatctttat gagatatatt tttaaacttg aaattccctt ctatttgtaa aaatctaaga
     5041 gtatcttcta tatcttcata ttccatgtaa aatggataaa taaatacagt gcttctttca
     5101 gcttgttcat aacctccatc cacactgtat tttatttcat ttgtagaatt taatattgct
     5161 acagcatttt tcacttcata tggattaaga aactccgtag acttaacatc atagtttttt
     5221 atacaactat tagccttgtc tatcacttta aacattttat ttttcaagtc tatatccttt
     5281 atgtgatttg ttagttttaa tttatccatc ctatcacctt agaatataac caatactaat
     5341 tgtcttagga acatcaataa tactattgca attaaaggag aaaaatctat aggtccattt
     5401 gtatatttat acattataga ccttattggc ccttctattg gctctgtaag attttccaaa
     5461 atattaaaaa ttttactttg tcttccattt ggaaaccaag tcatcaagct ttttataact
     5521 atcatccatg ctattatatc taaaagataa taaagtgcaa ttcttattgt tcccatatat
     5581 taaccgcctt tttactattt attttgccaa gggaagaatg ctttactttc taattctttt
     5641 ttcatatttg catctatatc cacattatta ggagctagta taaatattga tttagaaacc
     5701 ttttgtatac ttccatctaa tacataaact gccccattca taaagttaaa tatagattta
     5761 cgaacttcag aattagtcaa tccttccaaa ttcactataa ctgcccttct ttgttttaga
     5821 tggtctgcta tagttgcagc ttcatcataa accttaggtt caactattac aactttcatt
     5881 tggctagttg tatgtaaatt tacaatttta tttgcttttg cagtactaaa gccactgttc
     5941 atttcttctt cttgaactat atcgtccatg ccactttcat attcatcttc aatataatct
     6001 tcttcttcat caactatcca attcttaaat ttagatatta ttccatttga cattattaac
     6061 ttcctcctta aaatttattc aaatttgagt aattattttt tattgtagtt tctttctcca
     6121 aagattgatg ttccaactct aacaatggta gccccttcct ctatagcaat ttcataatcg
     6181 tgactcattc ccattgacaa aacatccatc cctacattag ggatgtttag ttcttttatt
     6241 tgttttgata aatctttaag acctttaaat acacctctta tctcatcttc atcttctata
     6301 aaaggtgcca ttgtcattag accctttatt tttatatttt tatatttttc tgacacttct
     6361 ttgacaaaat ctacaacttc ttctctatca agtccatgct tagactcttc tttagatata
     6421 ttcacttgta ctaagcaatc tatttctctg tttatttgtt ctgcacgttt ttgaatttct
     6481 tcgcataatg caattctatc taaagagtgt atcatacaaa ctttatcgat tatgtactta
     6541 actttattgg tttgcaaagt tcctatcagg tgccagttta atttgtcacc tataatatca
     6601 tactttctag ctaattcttg gggtttattt tcaccaacag aagtgattcc ttccctcatt
     6661 gcctcattaa ctaagtctac atcaacagtc tttgtgactg ccaaaagggt aatctctttg
     6721 ctgtctctat ctactttttt tgcagcttta tcaattttgt ttcttatctc agacaaatta
     6781 tctttaatag aacccattat tatcacctac tttatcctta tatttgtgtt tatactgtta
     6841 ggtcttaaaa ttattttgtc atatagatta acacttgtaa ctccatttat gtcattttta
     6901 taataatcca caaatacaga agattcattc tcataagtta ttccttctaa tatgacaaat
     6961 tttacacttt gtgattgttc atcaattaca tatacaccac gtttattgtc ttttgttttt
     7021 atagaagttt taggtatttt taaacattcc atctgattat atattatatc aaattcttcg
     7081 acacttgtat cctgatttgc aacaatttgg ttacttattt ttaattttac cattatttta
     7141 ttattatttt catatatttt gtaaactttt gcatctaatt tttgctcatg atattcaatt
     7201 attacatttt gatttttttc aaagttatta gaatcttctt tactgatgta tgtagataga
     7261 taattacttt ggtcattggc atctttttct atagttcctt ttacaggaat ctttgcatca
     7321 actttttcat tttttaacac caacgtatct acatttttgc taattatcat tactaatata
     7381 ttaaatacta aatatataag aataccaagt atcactaatc tggtatattt tacctttttc
     7441 aaagactctc cccctataaa attactttca ttataagtac ttcttctaaa aaatgtttat
     7501 tcctttttta taagctaata ttttacgttc taataattat tttttccata ttaaatttat
     7561 tttttatcaa tcttcctata caagactgca ttatttatat ataattgtat tataaagtat
     7621 aagtttaatt tacattttat acattaagct tactttatat gtttttatag ttatttttat
     7681 ttttagatgc aatataaaac caattaagat ttaaaataac ttttgttatg ttgacattta
     7741 tttaggtaat catttacgaa tcgggataac gttatcataa caaaagttta tgtcctaaaa
     7801 aaaatacaag gaggattttc ttatgtcaga aattttatta ggaacaggtt tacttttatt
     7861 ggttctatgt tgtttcacaa tttttagtta caaggcccca tatggtatga aggctatggg
     7921 agctctagca aacgctgcat gtgctacttt cttagtagag gcattcaacc tttctttact
     7981 tggtgatgtt ttaggtatgg aattttttag aagtgttgga gctgctaatg gtagtcttgg
     8041 tggagttgct gctgctatac ttgtgccttt agccttaggt gtatctccag tttatgcagt
     8101 tttaatagga ttatcttgtt tagatttttc tatattatct ggattcgttg ctggatactt
     8161 tgtttctttc ttaattaagt ttatagagaa aaaagtgcca cctggagtgg atttagtggt
     8221 tgtaattctt ctatgtgcac cgctatctcg tcttatagca tcaaatgtag acccattagt
     8281 taatagtaca ctactacaaa ttggacaagt tttgatagaa gctaccaaca ttagccctat
     8341 aataatggga attatactag gaggcataat aacagtagtt ggaacgtctc cacttagttc
     8401 aatggctctt acatctataa ttggtcttac tggtcttcct atggcaatag gagcattagg
     8461 tgcttttggt tcagctttta tgaactcaac actatttgca aaattgaaat taggttctaa
     8521 aaaagatatt atatctgttg ctattgaacc attaacccaa tcagacataa tttcagctaa
     8581 tccaatacct atatattcta ctaatttctt tggaggagcc ttttctggta tcattgtagc
     8641 cttgatgggt cttgtaaaca tgacaccagg tacagctaca cctgtggctg gttttgctat
     8701 aatgtttgct tataatccag ttcaacaagt tcttataaca gctggtttat gtgctttatt
     8761 caatgtaatt gcaggatttt taggttcaaa aatattcagt acctttaaag ttcttactgc
     8821 tggtgaaata cgtggtactg atgtagaaga agaaattgct taaaaaacaa taagatttat
     8881 aaatttatat ctcaagtagt agagttttat attgtaggca attttattaa ttgcaaacgt
     8941 tttatttttt atttatgttt atgattatta tgtgtgttga atatttagat aaatttttat
     9001 ttattatttt tatttagatt tttgtttagt tttttatttg gttgatatat taggaataaa
     9061 aattagtata gtttattatt agttagatag cataactttt tagatacata aaaaatttta
     9121 tattaattag atattattaa aataaaagcg atacatgaat ttttaaatgt ccatgtatcg
     9181 ctttattttt gccataatct taacaactga tttttaaagt ttattatctt aacgaagttc
     9241 attatcttat ataatattta aatgcataga cttctttaat ttaaccatct aaatatttaa
     9301 ttaaatatta ttatatgtaa tataactaat ttttaaatga atgtatagga gctggaatcc
     9361 ttcctcctct ttgagtaaat aattcagatg aatttgtact tataggcatt actggtgctc
     9421 gtcctaaaag tcctccaaat tctaccatat cacctacatc acatcctggg gctggtataa
     9481 ttctaacggc tgtagtctta ttattaatca tacctattgc tgcctcatca gctatcatag
     9541 ctccaagcgt acctgctgta gtatttcctg gcacagctat catatcaaga cctacagaac
     9601 aaatagcagt cattgcttca agcttatcta tacttaaagc acctttaata acagcatcaa
     9661 tcatacctgc atcttcagat actggtataa acgctccact aagtcctcca acatgtgaac
     9721 atgccattac tccacctttt ttaacagcat catttaaaag tgctaatgct gctgtagtcc
     9781 catgagtacc aacaacttca agacccattt cctctaatat atttgctact gaatcaccaa
     9841 tagcaggtgt aggagctagt gataagtcta ttatcccaaa tggaacatcc aatctactag
     9901 aagcttcctt tgccacaagt tgtccaactc ttgtaatttt aaatgcagtc tttttaactg
     9961 tttctgaaac aacatcaaaa ggctctccct ttactttctc taaagctctc ttaacaacac
    10021 cagggccact tacaccaaca tttataattc tatcagcctc accaacacca tggaaagctc
    10081 ctgccataaa tggattatct tctactgcat tacaaaatac tactaatttt gcacaaccaa
    10141 aaccttttgc atcttttgtt aattctgcag tttcttttat gatttctccc atttctttca
    10201 ctgcatccat atttatacca catcttgtag aacctacatt tactgaagca catactttat
    10261 tagtacttgc tagagctttt ggaattgact taattagtat cttatcacct tttgtatatc
    10321 ccttttgaac taatgctgag aatccaccaa taaaatctat acctaaatcc tctgcagcct
    10381 tatccaatgt ctgtgcaaac tttacatagt cattttcatc tgttgcacct gcaataatgg
    10441 aaattggagt tactgatacc cttttattta caattgggat accaaattca cgctctatat
    10501 cttttccaac cttcaccaaa tctttagcag aactcataat cttattgtat attttttctc
    10561 tagctttatc tccatcaggg tctatacaat ctaataaaga aatccccata gttatagttc
    10621 ttatatccaa ctgttcttca tctatcatct ttattgtttc aagtatattt cctacattca
    10681 ctattatcac ctccccttac tgtctactac agattatgca ttgaattaaa tatttcttct
    10741 cgctgaattt ttacatccac tgataatttc ttacctcttt caaccaagtc ctcaaccgtt
    10801 ttctcaaaag aatttaatga tttctctaaa tcaactatca taatcatagt aaaataatcc
    10861 tgtaatattt tttgtgatat atctactatg ttgatatttt gcttgtataa ttcatcacta
    10921 attcctgcca ctatacctgg tttatccttt ccaattactg ttaaaattgc tttcataata
    10981 atatcctccc ttattttttt ataaatctta tatttttaca aattgaacta tgtattaaca
    11041 tatatttcat ttatttgtta gtaaaccact ttttatgtat atatttaata tattttaata
    11101 cctatacatt catttgtcaa ttttttttca atattccatt atttttatac tatttagaaa
    11161 attcatattt tatatatgtt ttctcaacaa taaatacagt tttcataata atgaaaggta
    11221 ttacttagca atctcgtgat tttctaaata atacctctct aaaaattatt tttttaattt
    11281 tatgaattta attatacttt ttctagtatc aggatttaca attaatatag aaccaatcat
    11341 aattggtgtt agtatacaaa ataaaaaaat aatatctttt tttagattat attctttaga
    11401 ttctaattta taattattat ttattattcc atcataatca cttatatttg acttattttt
    11461 atttgtatct tgatttttgt tagaaatact ttccctataa ggaaaagaat gttcattggt
    11521 accaaatact gattttgagc ttaaatcata attatcatca ttttctgtaa ttggattttt
    11581 attgtaaaat cctttttctt tatcagaatt attttcttta ttaccgtctt taggtatatc
    11641 atgaggattt ttattttcat tgctatcagt acttgattct acatcatctt taggagtatt
    11701 tggttgtttc tcatcagaat tattattttc attattattg ttttgattgt tattactatc
    11761 attattttca tttccattat tcccatcttg attgttatca tcctcatctc tatccgggtc
    11821 gtctctacct cctcctccac ggtcaccatc aatatcatca ttttcccgtt catcatctgg
    11881 cgtctcttgg ttgtctccgc ctcccattat aaatcctggt ttaaaaacta tatttgaata
    11941 cccttctttt aatcctccta ccacttttac tctaaaacaa cgcaacttaa aatcttcaaa
    12001 aaaaagtata gcttgatctc taatatcctc ctgttcttca gatatccaat tgattccatc
    12061 gcttgaatat tcaacaaaaa ccttacttgc actatacggc atatcataaa ataaaacagc
    12121 tacatatcca ttttttgtat tttgaaattc cagttccaaa ttgtctattg tttttttctc
    12181 tataacagat acattcaaaa taggaactac atcatcattt attataattt tttctttatt
    12241 taacatttct gttttaaact ttcctgttat atcaaaattt tctccctttt ccaacttttc
    12301 ataataatca gtaaaatccc attctacatc tatatcaagt tgattaccac tagatgtttc
    12361 taataccata tatttgggaa attttatatt ttctatttta tcatctttac ttacttttat
    12421 ttcatcacta aatacaccaa ttcccctatg acaacatttc acaatattat agccagaatc
    12481 tttagctata gagggtataa gctcattata atcatcaatt atataaactt tcttactttc
    12541 tgatataatt gattgtgcca acttattatt atctcttagt aacccacttt cattgttgct
    12601 actatatgct tttattgaag tttcttctat ctcaacatca cctttagaat aaacacctat
    12661 agcatgttct ccattcactt caatatcctt atttagtatt ctaatatcat tttcagtata
    12721 aattcctatt gcatcttttc catttgcctt tattttttca acagattcgt aggatgattt
    12781 aatatgtaag tctcctcctt cttcaacgta taaggctgtc ccatcagtag caattatgtt
    12841 gtttatacca atacttatac ttccaccatt ttctatatgt attactcctt cttttcctcc
    12901 ttcacctatt atattcaatt cattatcatc aataataaat ctcccatgat tttttatcaa
    12961 tattttatat ttgttggtat ctattgtaaa attactgtca taaggaatat aaaatctaaa
    13021 ctcttcatct attaccatat tacaagaaag agtataaact atttcttctt tattattttt
    13081 attttcttga acccaccttt caaaatcttc aagacaattt tcctcttgtc caaaaacttt
    13141 atgtggaaaa cttataaaac acaaaactat tatactcata aaaataaata tctcatactt
    13201 tttcatctta cactcctaga aattttttag ctttgaaaca atcctgtcga aacatatact
    13261 tcacccacta taatttttcc acaattatca attattgtca atttatataa gtatctttat
    13321 taattgttat aaataaaaag ctacttacta aagtaaacag acgtttcact ttaataagta
    13381 gctatatttt tataacaaaa tagttataat attagaactt ccttcattta caattttttg
    13441 taaagtcttt tgcattttaa ctcttatctc ttctggcata gtatataatt tactttgtaa
    13501 ttgctctttt actaagtcat gtagtgactt accaaacata tttgattccc ataaatctgc
    13561 tggttgctct tcaaatactt ccataagata ttttatcatc tcttcacctt gattttgatt
    13621 tccaactatt ggagagacct ctgtagatat atctgctttt ataatatgaa gtgatggtgc
    13681 attggctttt aacttgatac catattgttt tccttgtttc attatttctg gttcttccag
    13741 actaagttct tctaaagaag gagctactac tccatatcct tttatcttgg catctattaa
    13801 agcactttct attttatcat actcattttt aactttagat agtctagtta tcagactaag
    13861 cagttgataa tctccttcaa ttttaaatcc acttttttct tcaaggacat tatagaataa
    13921 atcttgcttt gtctgtaaat caatatttat aacaccttct cctagctcta cattatcaac
    13981 acctgtatct tccaaaaact ctaactcaga aaatccttgt ataatacctt ctatatctct
    14041 tattttacct atatctataa tactttgttt taatgttgtt attatgctac tttttatcca
    14101 atgatttctt tcaagacctt caacccattt tggtaagttg attcttattt ctgtaagtgg
    14161 aaaatcatat aatacagctt ccataacctc ctctatatct tcttcttcca tttcaaccac
    14221 attcattgga agaactggaa cttcatactt ttcttccaat tcacttctaa gcatactcgt
    14281 ttcctcactt tttggactta acgtatttaa aaccactgca aatggttttt taagattttt
    14341 caattcttgt ataactctct cttctggttc tacatagctt tttctgtcaa ttccagtaac
    14401 tgaaccatct gtcaagacaa ctatccctat tgtagaatgg tcttttataa cttttttagt
    14461 tcctatctct gctgcctttt caaatgtcat agcttcttgt gaccaaggag tggatactaa
    14521 tctttgtttt ccaccttcct catgccctaa agctccttca actatgtatc caacacaatc
    14581 taccattctt acttttaaag atactgtgtc ttttattttt atttctacac catctgctgg
    14641 tacaaatttt ggttctactg tcattatcgt tttacctgaa ccactttggg gtatctcatc
    14701 tcttgttcta tcctttttaa attcattgtc tatattaggt ataaccaact tttccataaa
    14761 ttttcttata aaagttgatt ttcctgttct tacaggtcca actactccaa tatatatatc
    14821 cccctgagtc cttttggata tatcttcgta tatgttatta ttcatattat tcctcctata
    14881 catctaatct tattaaatta tatttagagt taagggttaa tattcttaaa atagaaaaat
    14941 aaagaccagc aatattaatt taatttacat atagaaattt gtaaatttca atgtaaatta
    15001 aattaatatt gctggtccaa atagtagtgt attatattaa ctgactttag tagtattttc
    15061 atttgactta ttattcatct caataagttc tttacgtctt tcttttgtca tgttcatcca
    15121 tataaatgta aacaccaatc caattatggc tactatagta agaactatga agtagtacct
    15181 atatccagtt acacccggaa atgcatccaa tatcttccct gctgctaaag gacaaagtac
    15241 ttctggaaga tatcctaatg tagcaactat tcctatagca gttcctgata ttgataatga
    15301 ataattacct tcttcaagta acgaatagtg taaaccttga attgcataca tagatacata
    15361 tattgcaaca catccaatca atagtatata aatcatagat gattttgttg gtgtaaatat
    15421 tacacctaaa agacctgcaa tcataagtat aaatccataa gatataatct ttgatgaacc
    15481 tactttatct ccaagtaatc cagaagcagc tgatgctact ggtctacaat actgagctaa
    15541 tattgagatt gcagatgcaa acaccacagt agctccaaat gcttcagttg aataaggcgt
    15601 aaaataataa aatgacatat tagctgaata tgaacaaaac attattattg ttattatcca
    15661 tgtatgaggc atttttatta ctgcaaaaaa ggcttcttta tcaaattttg ctgatacctc
    15721 tttttcatta tcctttaatt tgaaaaatac taatactcca actattacat ttattgccga
    15781 gtataatact ataatagctg taagaccaga tttatcactt acttttgatg aaatataccc
    15841 aaatagtatc aatactagag atagatgaat tgcattggta attccacgac cactttccat
    15901 aaatccaaaa gcttttcctt gttcattttc agatgctaac attctaacag cttttacaag
    15961 agctggccaa aaagtaagta ttgttgttat tccccataag gcatggatta aaaacacaac
    16021 aatatatgga ggataaagtg ttaatacaag accagatatt cctgtaataa ttaaagaacc
    16081 tgaaagcaat tttctggctg aaatacggtc tgctaaaaaa ccaccaaaaa ggtatgatac
    16141 cattgcaagt agaccataaa tgcttcctaa gcttcccatt tgtgtatttg taagattaaa
    16201 tgcttgtgca aaagtatcat aataataatt ctttaaatat ggtaaagcat atataaatgc
    16261 tcctgctaca aagagtatta tcaagacttt gaaatttttc caaatgcttg gtttatttac
    16321 ttcaactgac atacttcctc cttatgtaaa tacctgtaaa ttttcatgat aattaattat
    16381 tgcttatttt attgtttatt caatatttcg gaatatttat taatatatta ggttaatcta
    16441 tcaataattt atggtgagtt atgagctaaa agtaaactct tttagctcac cataataatt
    16501 atatttttat ttatactaac gtccagagtt attagatatc tttttttcat agttcttcca
    16561 tagcattaaa cctgttatta taacaaaaag acctcctcct atcatttcaa tctcatagat
    16621 tatattatct tttaaagtct ccataactgg tgccgttgag aaaagtatgc caactacagt
    16681 tactacaagt accattctgg caatattgat acaaactgaa tcactttttg caattgaata
    16741 tggtctttct tcatttggtc ttgtctttct aagtctaata taagatgtaa aaagcaagac
    16801 atatggaaat aatgctgtta atgctgtcat agtaacaaga acattgtata tattatctac
    16861 tgatggtaat aagtttgttc ctacaagaag tagactcact attatagcct gaactattac
    16921 tgcatttgat ggtatattgt atttatttgt aacagtcaat tgcttaggaa atacaccttc
    16981 tcttacattt ccaaaaagca tctttactgg acctgctata taaagtatta atgctcctag
    17041 tactgataaa gttattccaa atgctactat tcttataaac caacttccta tacctaaatc
    17101 ttgtgcaact ttagctaatg catctagtat ccctgtagac gctgcaattt tatctggagg
    17161 aagtatcatt gttattgcta ctgaacctaa gatatagatt cctcctatta aaacagccac
    17221 agttaatata gctttaggaa agtttttctt agcattatcc atttcggtta tgaaattagc
    17281 agtcgtttca gctcctgata atgcaaatat tatagcagat atagaaacta atgaattggc
    17341 atctattttt ggtattatat tttggattgt atatatcgat gctgatggag cttttttgag
    17401 aataactaca gacataaatg ccatcactat aagtaatata gcaggaattg tagaacctaa
    17461 tgctcctgta ttcgtaaaaa attttccaaa tgccatccca cgagtactta ctaaggacaa
    17521 cagccaaaat attatgagtg aaagtgctaa aataaacatc ttattgtttg ataaatctgg
    17581 atttccaagc atatatgcaa tatttatagc taaaaatgtc aagaaagaag aataccaaaa
    17641 tatctttgat gtccaattta gccatgacac cataaatccc cacttctctc cataggcttg
    17701 tttaacccat tctccaagtc caccatcttt tggatatgtt gcagccaact ctgcacatat
    17761 aagtgatgca ggaacaaaga atataaaagc aaacaataac caaactggta tagaaccaag
    17821 acccaatcca aaactagagg cagtagattt agctatccat ctaatcccaa atagtgctga
    17881 tatattcata aatactaaat cccaaaatcc aattttcttt ttcactgtaa ttcctccaat
    17941 ttcaaattta ttcaaactca tacacaatgt attgatttaa atatcaagta taagttcttc
    18001 aacacttctt ggataattag ttataattgt agcaccattc ttacctacta ttgctgtatc
    18061 tgagtgtcta aatcctccaa ctccaggtat atatatccct ggctccatag aaaataccat
    18121 tccttcttct aatattaatt cattgtcaaa tcttaaatat ggctcttcat gttctgataa
    18181 acctaaacca tgaccaattc tatgatttga atataactct agaccatact ttgcaacaac
    18241 agctcttgct gcttcatcca ccattcttgc tggtatacca gcttttattg tatctaatcc
    18301 agcttgttga gcctctactg caatcttaaa cacttctttt tgtctttctg ttggtttacc
    18361 tataataaaa gttctttcat tttctgctct atagttttca taccaaacct gtctactatg
    18421 tattactata tctcctcttt gaagtatcct agtattggaa tctaaatgag gttgtgctgt
    18481 tctatcaatc cctgaacaag tccagtttgc aaatcctata tatgtatcag gataattttc
    18541 agaaacatat tttagtaaag cattatcccc cgcaacgtca aactccaatt cactcatccc
    18601 aactctaaca ttttctaaag acccttttat tcctatatca gataggtaac ctgctatctt
    18661 caaaaattct atttcttttg catcttttac atatctcatt tcaaatattt tacttccaac
    18721 atctttgata gcaaatgact tatcagttat atactctgaa aaacttgcag atactatatc
    18781 tttttctatt ccaacagttg ttcctttagg gtatttattt aaaattatat ctaagtaatg
    18841 tttatgtgat attccatgtt ctttcatttc tggtatttca taatacacat atacattatc
    18901 aacttttgca acttctttag catgtaattc ttctaatcca ggtactatta attctatttt
    18961 atcttgagtt actactgtta caattggtct agaataagtt attgctttaa atccactaat
    19021 ataaaattga ttgtctggtt caaatattaa agcaatttca atgctttttt cattcatgta
    19081 ctttctcaat ctttctagtc tcatattatt tgacataatg cacctctttc ttaatattca
    19141 gataatttat tcaatacaac aaggataaat atattatttt aaaattctta atatttttag
    19201 tacttgtcgt atttgcatgt aaattaattt tatttgtgat tattttatca taatcatgat
    19261 tattttgtca atcacgattt tatttttaaa atttttttat attctagtga ttttaaatta
    19321 tctaaaatat aacgagtata cattgtattt tggaatatta ttcttgattt atggtaagtt
    19381 taatgataaa atcacgatta tataaactat attgattatt tgtaggtgaa taaaatgaca
    19441 aataaagaat tacaaaagaa aaggatattg atgtatttca tagaagctgc tcaaaatatt
    19501 atggaaaacg aaggtataga aaatattaca cttcgtaagg ttgcagatat ggctggatat
    19561 aatagttcaa ctctatataa atactttaaa aatttagacc atttaatatc atttgcatct
    19621 ataaaatatt ttaaagatta taacttgaat atttcaagat gtatagaaaa tgtcaatgat
    19681 gaatataaga aatatataat tatgtggaaa ttattttgta agcattcttt tgacaatgct
    19741 caagcttttt atcaggtatt ttttaacttg tctagtgatg aacttagcta tataacaaaa
    19801 caatattatg atatgtttcc agaagattta ggtattcatg atagtgacat atctttaatg
    19861 attacaggag aaagtataaa ggaaagaaat aagatactat taaacaatct tgtagatact
    19921 ggatatatac cagctaaaga tttagacata atcaatgata taattatttc tttttatcaa
    19981 aatctattat tcttaaaaaa gaacacagga aaaaattctg gtgatgatga attaactatg
    20041 aaaatgattt cttctattga atacataata aaacatgcta aataaaatta aaatatggct
    20101 gtctcaaaat agagataagc cttttttatt gttgaaataa taaaaatata tttaatttca
    20161 tataaaaaaa agtgcctcgt aaactaaaaa gttcattttg agacacccct aaaacaaatt
    20221 tattatttat tatacaatag tttatatatc atcacctaaa actacttctt ccatttcatg
    20281 ctttttactt ctcatcatta gttctattcc aacttcttta ggatttgaac ctttatttat
    20341 tacagaatat atagcattag ttataggcat atctatatct aatttttcag caacatcatg
    20401 tgctacttca gtagcagtta tgccttctac aaccatcttt acttctttta aagtatcttc
    20461 taaactcata cctttaccta tgagtatacc agctcttcta tttctactat gcatacttgt
    20521 acaagtaact attaaatctc caatacctga aagacctgca aaggtagaca tattagctcc
    20581 catagcaatt ccaagtctgc tcatttcact aatacctctt gtcataaggg cagcttttgc
    20641 attatcacca taccctaatc cgtcacatat cccagctcca aatgctataa tattttttaa
    20701 tgctcctcca agttctactc caactatgtc tggattcgta taaactctaa gtttagggct
    20761 cataaataaa tcttgtatca tctgtgctat ttttaaatct ttagaagcaa ctactacggt
    20821 agtaggaata tcccttgcta cttcttcagc atgagatggc ccagatagta ttacatatgg
    20881 attttgtggt aattcttctt cacagacttg agataatcta agcccagtcc ctttttcaag
    20941 acctttagct acattaacaa taacttgtcc ttcttttata aatggtttta ttttttggca
    21001 agtacttctt acagcctgtg atggaactgc taaaactatt accttacaat cttttatagt
    21061 tttttctata tcagtactta tagttatatt gtttggaaat aatactcctg gtaaatagtc
    21121 tgtattttct ttagttctgt ttattttttt agcttgttcc tcttttcgag tccacatgct
    21181 cacatcatta cctttttttg caagccctaa agctaatgca cttccccagc ttccagtacc
    21241 taatacacat actttctcca tgtttaacac cacctgagta taatatattt actattcttt
    21301 cttttgacca atttttctct ctgtcccatt taataatcgc tttatatttg ctctgtgatt
    21361 atatattact gatgcagtta agaacagagc tactattaaa ccagctttat tttttaccat
    21421 aatcataaat attggtgaac atcctattcc tacaacagaa cccaatgaaa catactttgt
    21481 tatagctacc accaatataa atactgctag acacattagt gttattactg gatttacagc
    21541 tagcattgca cctagtgaag ttgcaacacc tttacctcct ctaaatccta aaactgctgg
    21601 ccaattatga cctgctacaa cacatataac tgccagatat ccagctaaaa gagtatctat
    21661 tccaaccaat cttgctgcaa attctgatat taaaacagcc attacacctt taagaacatc
    21721 acctaaaaat gtcattgctc ctgctctttt tccaagtgtc ctaagtacat ttgtcgaacc
    21781 tgcattacca gaaccttgag tccttatatc aaccccagca attcgctttg caactatgta
    21841 tgaggtagat atatttccta aaagatatgc aactacagca ataattatat aactaaatat
    21901 ctccactgaa tttcccccct ttagttatat tttaagatat ttatttttta tttttttgtc
    21961 tatactcaaa ctttatggaa gttccttcaa atccaaaatt ttctcttatc ttattctcaa
    22021 ggtatctttg atatgagaag tgagttaaat ccttatcatt tataaacaat gtaattttag
    22081 gaggtctaat gtcagtttga gtcccatagt aaatttttag tcttctacct ttgtcagatg
    22141 gtggttgatt taacattaca gcttctccaa ttacttcatt cagtgctgaa gttgatattc
    22201 tcttagaatg ttcatttgac acatattcaa ctgtatctag tatcttgttc attctttgat
    22261 tagtttttgc agaaacaaat acgattggag catacatcat aaatggaaat ttttctttta
    22321 tatcttttgt gtaattactc atagtcttat tatctttctc tattaaatcc catttattga
    22381 ttacaaatat gcatcctttt ccttcatcat gtgctatacc tgcaaccttt gtatcttgtt
    22441 ctgttacacc ttctaaggca tctattacta tcaatactac atctgctcta tctacagcac
    22501 tcatagctct tattacactg tatttttcta ttgtctcata tattttactt tttcttctaa
    22561 gccctgctgt atctattaga agaaatttct gtccattttt ttcaaaataa gtatcaattg
    22621 cgtctctagt agttcctgca attggactta caattactct ttcttctcca agtatcttat
    22681 tgagtataga actttttcct gcatttggtt tacctgttat agctactctt attatatctt
    22741 cttcatactc tgtatctaat cctgctggaa aattttcaac tatctcatct aataaatctc
    22801 caaatcccat actattggct cctgaaactg caaaaggcgt tccaaatccc aactcataaa
    22861 aatcatatat attatcaaat tgactttgac tatcaatctt attaaccact aagattactg
    22921 gtttttttgt ctttctaagc aattgtgcaa tttctttatc agcagctgtt attccagctt
    22981 taccatcaac tataaaaagt attacatgtg acatgtccat tgcaagcatg gcttgatttc
    23041 tcatttgaga taatattata tcttcgctat caggttcaat tcctcctgta tccactaatg
    23101 taaaatattt atctaaccac tctacttcag caaatattct atctcttgta actccaggtg
    23161 tattttcaac tattgatatc ctttttcctg caaatttatt aaatattgta gacttaccaa
    23221 cgtttggtct cccaactaca gctacaactg gcctacttat actcatttta tttctccttc
    23281 catcttttgt tttctatttt aaaattttat ccacaaagtc ttttccttca ttcaaacaag
    23341 ggataatttt tattctcata agttcttcta attgatttaa ttcaatatta tctaagaaaa
    23401 tttcttcatc tgcttttagc atacttcttg ttatataaag agcttctcct aattctttat
    23461 cttcaagttg ttcttttaaa tccttagctg ttaaaagacc tgatacagtt attgtctctc
    23521 caaaaaactt gtttttgatt tcatatacat ttacttgaag atttttaaac ttgtccatca
    23581 tagcatctgc catactctgt ataaattcat atgcagaatg accagtagct attgatactt
    23641 tcttctctct ttttagaata ttctcaggca atatatttag atactcttca atctctgttt
    23701 ttaacttacg tatcatacct acaccatctt caaactgtaa aaaaccttca tattcttcat
    23761 atccaggcaa ttcactatta gatagtatat aaaattcatc tgataaaaat gcaaatcgag
    23821 tacctaattt ttcaagatat ttctgttgta tttgatgtat ttgctctata gttttacttg
    23881 cgctcttatc attaaatata tttaattcta ctagattttc tctatgcttt gtaatcccaa
    23941 ctggaactat cgcaacactg tttacatatg gataaagttg agctagttct tttattgttc
    24001 tatccaattc ctttccatca tttatacctg gacataaaac aatttgacag ttcatttcta
    24061 tatgagcatc tgccaatctc ttcattatac catataattt tcctgcaaat ttattgctta
    24121 tcattttttg tcttaactct ggattagttg tatgcacaga tatatttata ggacttattc
    24181 tatattttat aatattattt atatcttctt cactcatatt tgtaagtgtg acaaaatttc
    24241 cttgtaaaaa agataatctc gaatcatcat ctttaaaata aagagtttct ctcatacctt
    24301 ctggcaattg gtctataaaa caaaacatac acttatttct acagctttta gcttggtcaa
    24361 ttatcggatt tgtaaattca acacccaaat cttcatcata gtctttttct atctcgtata
    24421 tgtagacttc gccatctttt ttttgtattt ctacatctaa atattcatca cttagtaaga
    24481 atctatactc tattatatca tgtattggct gttcattcac agatataagt aaatctccta
    24541 cctctatacc tatttcttct gcaatactat ctttataaac cttacttata atattattta
    24601 ctttttttac atttacttcc aatattttca ccactctttt gtattcatca atgcttatta
    24661 taatatattt taatatgcaa tttatttatt tattatattt tatgatagca tttttaagca
    24721 tttcatgtca taataacaca ttttaattct aatagtcaat ataagtaaat agctataaaa
    24781 tagattagaa caaataatat tcaaatttaa gtttcttaaa aatttgaata ttatttgttc
    24841 ttaaaaaaat tttataaatt taatgtttta ctatgattaa cgtcccattt gtcatatcat
    24901 gaaccattcc acctacacac tttgacatca acaatgcctt attttgtaag tctttctcat
    24961 catttgcata aaatattgga caacctccac catttttcat attcttatca agtgtaacta
    25021 tagctagggt atattcattt aaacctatat ccattcccat cttatgcact tcctttcttt
    25081 cctattttat aattttttag tgataagttt tttcctttag agcttgaaag tattgggcat
    25141 gactttacag cttccattac tttttccata ttcttatcta ctggtaaata agcaatcatt
    25201 atactttcat cctctttatt tcttctaggt aatggtacga atgaaggttc tccataatct
    25261 ccatataaac ctaaccttga atatatattg tatactattg cttgtctttg accggagtca
    25321 aataatattc ctgcattagc ataatcctta tttttaggaa ttatttgtat tcctacacct
    25381 ctattaatat atacttctct atcacttttt agacctatat ttgttatacc ttttaaatct
    25441 cccaccttta gtatagattc gtccacaaaa ctaatttcag caggcattac atcagctatg
    25501 tctccaatac tttttctagt aagaagtttt tttaatataa aggctaacac taatccacaa
    25561 attgaactta ttaccaaact catcccttca cttattttcg tttctgcaag tattacataa
    25621 tacaatccta ttgttaaaaa agatgtaact atacacatat aatttcttac ttcataggtt
    25681 ctagctatct cttctatgaa tgcagcacct ctctttacaa gttgaacttc ctctaaattt
    25741 tgcaatgtat ttcttctatt tgctctcact tgtctaaatt gttctgctgc tagagataaa
    25801 aatgttatgg atgtataaga acgatttata agagctggaa ccaataatgc tcctaatgaa
    25861 gaagcgacga atgctaatat aagttgagat agtaaaacat ttggttgcgt tggatactgt
    25921 ttttggtcta aattcaacat tataattcta gatgttatac caataataac agcaacaaaa
    25981 aatactctat ccattaaatc actcctaaaa tatttaattt gaaattttac atgtaaatct
    26041 acaaagtctt tctagaaaaa tcactctgct ttccttttga tgtttctatg ataggtgtac
    26101 ttttcactac atctatcatg acatctatat ctttgagtat tggcatatat ggtataacca
    26161 cagtactttt ttctaaattg gtcttagata tagcaacaat atcaacctca tctacatctc
    26221 tatctatccc catatgaata aatagattat gtattatagc ttgtctttgc cctatatcat
    26281 ttactattcc aaatgcacct aaatctcttg gtataacttc tatggccaga cattcatttc
    26341 tatacttttc tcttgtactt tctagaccta tattagttat tattacatca tttaccatta
    26401 aaataggtcc ttcaaaactt atttttgcag gaactatatc tgctatatct gctatactgt
    26461 ttcttcttaa aaatcttcta aaaatcaagc caacaatagc tcctgacaca atagctaaaa
    26521 ttgtgcaatg aaaaaagcta aggtcatact ttcttgcaaa aacgatataa actatcgaag
    26581 atactagtgc agaaaaaaga cttatataac ttctagattc atacgtagat gctatttctt
    26641 caacataagc atctcctttt ggtactaatt cttcattatc aatattcttt agtgttattc
    26701 tttcttgttc cgcaagacct tgaaactgtt gtattgctac tgcaaaaaat gtcaaggctg
    26761 caaactcctt atctataagt gcaggtaatg ctatagcacc taacgaagct gaaagcccag
    26821 atataattat ctgttctaaa taatcttgtg gtcttgatgg gtattgtttg tctgtaactc
    26881 ttaaaactaa tcctctacat aatattccta caactaatgc aactataaaa gagtgtctaa
    26941 ataaatttgc attcaataaa ctttcttcca ttttctcagc tccttctctt atatagtttg
    27001 tgcaaaaaac aaaaaaaatt cccttctatt taaggaaatt tttttgtaat attgtaacta
    27061 taaattcgct accaatgcca agttcacttt ttacttttat acatccattt agtgatttga
    27121 ctatatgctt tgttatagcc aatcctaaac ccgttcctcc tacatcccta cttcttgctt
    27181 tatctaccct ataaaatcgt tcaaatattc gttctacatc ttcttttggt atccctacac
    27241 cattgtctat aactttaatt acgatttttt cttcatcttt taacactaca acttgtacaa
    27301 tcccattatc ttgggtatac tttatagcat tatcaactag gtttaagaac acttgcttta
    27361 tatagtcttt attggatagt atacttattg atttatcatc acatttatat aaaacttcta
    27421 tatttttttc actagctgaa tgttgagtta tttcatatac ctctttaaat acatcatata
    27481 ctacaacatt ttccataaga agagtatctt tattttctac aaatgataac agcaatatat
    27541 catcaatcaa cctttttaat ctatctgatt cgctttctat tatttctaag aatctatttc
    27601 tagtattcac atctatattt tcatttgact ttagtgtttc aacaaaccca cttatagatg
    27661 taagtggtgt tttaagttca tgacttacat ttgcaacaaa atcacttctc atattttcaa
    27721 gtttaacttt tttagtaata tcttctatat ttactatcga acctattatt acgttgttag
    27781 tattctgaag ataaactggg tctaccttta tcttatatac gatatcatct ttagtagtaa
    27841 tattagcagt ttcattatct ttagaaccaa taaattgaag tatttgtttt aatatttctt
    27901 cttcttttat tacaacatta atattatgac cttcaactaa ttgatttggt tcactcttta
    27961 tcatttcttt tgcttcatca tttatcagaa gtatatttcc atttacatct atagccaaaa
    28021 ttccatgaga tatactcttt aatatagatc ttaactgtaa atgtttatac tgtacttctt
    28081 ctaaggtttt atccatattc tttatcatat gattaaaatt tctagctaat tcaccaagtt
    28141 ctcctttggc tgatatgtta agcctagcat gatattcatt attacttact ttttttgaga
    28201 cgtatataaa ctcttttaaa tatttcctta atgttattgt atatcttaca gctaatataa
    28261 caatggctat ggacaacata acaataaaaa aatatatact atttaaatct tccacaagat
    28321 aacactccta aaatataacc tttttaatct attttatacc ctacacctct tatagtctct
    28381 atatattttt ccgatttatc ttcatcttct attttttttc ttaaatatct tatatgaaca
    28441 tctactgttc tagtttctcc atagtattca tacccccata ttttatccaa aagataattt
    28501 cttgaaagta cttttccttt attttgaatt aataatttta acaattcaaa ttccttaaga
    28561 gttaactcta tcttttcaga gcctttggta acttcatgtt ttgataaatc tagttttaaa
    28621 ttgcctgtag taagtatatt gttttcttca cctaagctag tcaaattata tctccttaaa
    28681 acagaactaa tcctagctaa caactctttt atactaaatg gcttagttat atagtcatca
    28741 gcacctattt caagcccttc aaccttgtca ttttccatat ttttagcagt aagcattata
    28801 acaggaatat tttttaagtc cttatcactt cttatttttc tcaaaacttc tattccactt
    28861 atatttggta acatccaatc taaaagtatt aaatctggtt taatttcctt agcctttata
    28921 aatccgtcaa atccatcata tgaataacta acttcgtaat ttgacacctc caaattgaat
    28981 tttaatagct caactatatg catttcatcg tctataacaa gcactttagt attcattaaa
    29041 aagctcctct ctattttatc tgtagtaccg ttcctattcg ttctgatgcc ttattatgtt
    29101 ttctataaga gtggaattta tctgctctac aactagtgca taaatttaaa tttattatat
    29161 tttctttttt aactccacta catctaagca tatactcatt aattttccat aaatctaaat
    29221 agtaacttcc ctcttttatt atatagaact tttcatcatt atttgtaaga attgtgttaa
    29281 atttttctac taaatcttca gacacttcat agcaacatgg tccaattgat ggcccaatta
    29341 tacatactaa atcttcagga actgttttat agcattcaga cattaatttt attgttttat
    29401 gacctatatt actaaaagtc cctctccaac ctgcatgagc cagtcctatg gctttatttt
    29461 tagcatctat aattgatata ggtacacagt ctgctgtaaa cagtaaaagt ggtacttttt
    29521 ccaagtttgt tataagtgca tctccatctc ttcttttccc tatagtatct ttattgacta
    29581 tattaactat atctgaatgt atttgagaat tactagttat attttcaaat gtcaaatcac
    29641 attctttaca tacttttaac atatcttctc tattttttgc atctatattc ttacatgtta
    29701 taactagatt tacagaattt aatttttcat caagttcttc acaattacca cttaaattaa
    29761 catactcttt aatatcttgt acatttttta tatttatata atctttcatc atttgtctcc
    29821 tttttctatc agtatacttt taagttcatt tacaaaagta tttatatctt taaattgtct
    29881 atacacagaa gcaaatctta cataggcaac ctcatctaac tctttcaatt tatccattac
    29941 catctcaccg atttttttgg tttcaatttc ttctataaaa catttattta tttcattttc
    30001 aatatgtaca acaatacttt caatctcttc aatagaaaca ggtctttttt cacatgcttt
    30061 tagcaatcca tattttatct tttctctatc aaaatattct ctactattat cctttttaat
    30121 gaccattaaa ggagttgttt ctattttctc atacgtagta aacctctttt tacatgattc
    30181 acactcacgt cttcttctga tagattttaa atctgtatgt ctagaatcta ttacctttga
    30241 ttctttataa ttacaataag gacattgcat ctatattcct cctttgttta actatatctc
    30301 aaactcatct aagcttaatt ctgaacctat atttactatt attgtatcac aacctatttt
    30361 aagtatatca ttccaaaata taacttcttc atcccctcct cttgaaaaaa aacttctact
    30421 tccatctcca aaaatggtaa atgagactac cttaccttcg tgaatatcca tgtctatgtc
    30481 tattataaac cccatccttt ttccattttt tacatttata atttcttttt ccattatatc
    30541 agaaactcta atcatattgt actcaccctt tctttaaaaa taaatatgta tcgaagtaaa
    30601 aaaaaatgac ctatataggt catttaaatt aaaataatgt ttacagtcca tggattactt
    30661 atacatattt tctcatattt tttaaagcat ttttttcaat tcttgatact tgtgcttgag
    30721 atataccgat ttcatcagca acttctatct gagttcttcc tttgtaaaac cttaaatcta
    30781 ataccaactt ttctctacta tttaatttct taatagcttc tttaagtgat atttcttgca
    30841 accaattttc atcagtatct tttttatctt gaacctgatc cataacaaat attgcatctc
    30901 cattgtcttg atatactggg tcaaacagag atattgggtc ttgtatggca tcaagtgcca
    30961 ttacaactga ttctacttct aattctaatt cctttgctat ttctgatact gtaggttctt
    31021 tagaatttgt tctaattaat ctttctctaa cttgtagtgc cttatatgct atatctttta
    31081 atgacctact tactctaatt gggttattgt ctcttagata tcttctaatt tctcctataa
    31141 tcataggaac agcataagtt gaaaacctta cattttggct taaatcaaag ttatctatag
    31201 cttttataag accaatacaa cctatttgaa ataaatcgtc tatattttct cctctgttgt
    31261 taaacttttt tattacactc aagactaatc ttaaatttcc tcttacaaac tgttgtctgg
    31321 cttcttcatc accatttttt atttgtaata aaagttcctt catttgctta tttttaagaa
    31381 ctggaagttc tgatgtatta acaccacata tttcaacctt attaacttgc atatattttc
    31441 agtcctctct caaaagtatt catctattga aattatttac atttccaatt tttttatact
    31501 ttttaagaag aattaattta acattataca aattttttca tttctttttg caaccttgag
    31561 attattttct tttcaagtct agatatgtag gactgtgata ttccaagcat tccagcaact
    31621 tctttttgtg ttttttcaat acctttatca ataagaccaa atcttaattc cataatttgc
    31681 ttttctctgt cacttaatct gtcaagtgcc ataaccaaca aatctctatc tatttcttct
    31741 tcaattattt tgtatatttc atcattttct gtacccaata catcagataa taaaagttca
    31801 tttccatcta aatctatatt taaaggttca tcaaatgata cttctgtttt ctttttatta
    31861 ttttttctta agtacattaa tatttcattt tctatacatc tcgaagcata agttgccaat
    31921 tttatatttt tattcagttt aaaagtatta actgctttta tcaaacctat agttcctatt
    31981 gaaatcaaat cttctacatc tatacctgta ttttcaaatt ttctcgatat gtagactact
    32041 aatctaagat ttctttctat taaaattgac tttacacttt catctgtttc tagcttttgt
    32101 aaaagctcca tttcttcttc tggtttcaat ggaggaggaa gtatatttgc ccctcccata
    32161 tagtatattc cctttggctt tattattttt aatttaatac ctaacatagt aataaagctt
    32221 attattcttt ctttcaatcg taacattcct tgtccccctt aaattttcac ctaaaatcca
    32281 ggcaatatat ttggattcat tattgctcca tattctctat cattaaaatt tgaaatacca
    32341 agtattatat ttcctatctt ttgctcatct acttctatat agtcagcttt taagcctatt
    32401 attatgcttg tcttattact tccagcatgt ttataaggta ttattctaac tctacctgaa
    32461 gttttatcat ctaaacttcc tataatttct tgtgctttct tcatatctat atcttcataa
    32521 ttaatattta atagtttttc tggaataaat ccttctaaca cactagactt aactattatt
    32581 acatcacttt gacttattgg gtcttttaac aaattaccac tatctactaa agctttacag
    32641 caaaaacttt tttctagtaa gtttatattt atagtctttg taagctcctt aatgtactta
    32701 agcattttca aatctttata aatgtatttt aataattcac aactcacata agtacatgca
    32761 attaagaaag atatttttaa gtgacttatt cctgtaaaat aaattataaa ataagtgctt
    32821 ccagaaataa aaatgtttac tagataaaaa actaacgcta catgaacata atcttttttg
    32881 ctcttgcttc taaatgaaat taaagttatt atgctcatga ttattatttt aaatggtaca
    32941 gtaaatagta tattcattga tggatacaaa taagcaacag aataggcact tcctaaacta
    33001 gcccccaata acttattttt tttaggatta tatcttttag taagaataga agtacaactt
    33061 ataataatat aatttattac taggttttct ataacatagt actcaatata caccatgtat
    33121 ccccccttaa aacctttaaa catattataa gatagtatgt acaaaatttt atgtcacttt
    33181 tgatggtcga atccaaaaca aattctattt aaaaagaaca atgatatatg tcttatactt
    33241 tatgtcgtca aaagatattt ttttaatgcc aaacagatag acttttttgt agaatccaat
    33301 atatctaggt agaagttatt cgctttactt ttggtatatt tataaaaaaa agactacata
    33361 atttaataat atatcaagta aatcttttac ttatataaaa tcaaattata tagtctgtaa
    33421 attcaaatat aaattattgt gctagtaatc catttgcata ttttgcagat tcatttagtg
    33481 cttgttctgg agttatgtct ccagctatca tcatttgaag ttgtttccat aaatattcat
    33541 atatctctac tccatgaggt ccaactgtca atggtctata catatgttta tctcttgtca
    33601 ccatttcttc aaatcttggg tctccttggt atttttcacc tttagcaact ggagctctag
    33661 gtatttcttt gtggaaagct tccattgatt ctttgcttaa catatactca agaagtttaa
    33721 atccaagttc tttattttta catgctgaca ttaatgttaa atcatctact gaagcaaaac
    33781 ttccaactgt cttatcttct aatgatgtta catatcccca gtttaaatcc gggaatgatt
    33841 tatcaaatgt acttgtagca gcagaggata gcattatagt aaatgcagct tttcctggtc
    33901 caaatacact ttcaatcatt tcattactat cttttgataa agcatcttct ggaatatact
    33961 ttttcatatc atttataaag tttgcagctt tcaagccttc tgcattatta aatttaactg
    34021 attttaaatc atcattatat atatctccac cagcttgcca taaatacgga taccagttcc
    34081 agtttaagct tccaaatact ttagaacccc aaccttgtgc aagaccccac tggtcgattt
    34141 ttccatctcc atcagtatct tttgttgctt tttcacagat tcttttaaaa tcatcccatg
    34201 tttttggagg tttttctcct aatttttcta gtatatcttt attgtagtac aatacgcctg
    34261 gattagcagc ttctataggt aaaccataaa taccacccat catttttgca tcatctaagt
    34321 atagataatt atctgaagtt cctgattttt ctaaataagg tgttaaatct tctactgcac
    34381 ccatttctat aaattgaggg aacatctctg catacatgta tcctatatct ggaccttcac
    34441 ctgcacttat tgctgttgca tatttttctg cgtagttatc ccatggtact atctgaaatt
    34501 caatttcaca gttattttct ttttcaaatt tttcaaatat cggcttccat gcatctgcat
    34561 catggtctgt taatggtgca gaccatacaa ctaatttttc tttaccctct ttgcctttag
    34621 aatcttcttt ttttgaacat cctgcaagaa ttgatactga tagaatccct accataacta
    34681 aagaaagaac cttttttagt ttaatcatgc ttaatcccct ttcagacttt aatttgtctt
    34741 tttcctcaaa tgtttacatt taattcagat ttaatattaa caaataagac tactcttgtc
    34801 taatcataaa aaactatact aactcctata actatatttt ttttctatac ctattagatt
    34861 taattcatac taaaaactta ctttttaaat tttatcaata tttaaaaata tttttaagtc
    34921 tagccacaaa ctaactatct aaattgaaca aaataaaaag aagtctattg acttctttat
    34981 aagcttacat attatcttct tcttcttagg aatgtaggta tctccatgct ctcatcatca
    35041 tcttcttcaa ttatacttgc ctttttagga ggctctattt tttcctcagc aattacttct
    35101 tcttcaattt cttttacagc ttgttttact gttgtattta atgaagaacg tatgcttgat
    35161 ttaggtttag tatctaaatc taaggcacca ttttgtaatc cttcaaatcc tgttgcgatt
    35221 actgtaatca ttatttcatc acctaaatct tcttttatag aagctccaaa tataacattt
    35281 gcttctgggt cacaggattc cataactaaa gttgatgcct cattaacttc aaataatcct
    35341 aagttaggac cacctgtaac attaagaagt actcctttag ctccttgtat tgatgtttct
    35401 agaagtggag attgaatagc ttgtcttgca gcatcaattg ctctagtttc accactagca
    35461 ctacctatac ccatatgagc aagtcccttg tctttcatta tagtagtaac atctgcaaag
    35521 tccaagttta ttaacccttc aactgctata aggtctgaaa ttgattgtat accttgtttt
    35581 aatacatcat ctgcaactgc aaaagcatct aacattgatg tatttttttg tactatctgt
    35641 aaaagtctat catttggtat cgttataaga gtatctactt ttgatttaag ttctgctatt
    35701 ccaccttcag cgttcttcat tcttatctta ccttcaaatg caaaaggttt ggtaactaca
    35761 cctactgtaa gtatccccat ttcttttgcc aagcctgcaa ccactggagc agctcctgta
    35821 ccagtaccac cgcccattcc ggctgttaca aagaccatat cagctccttg aagtaacttt
    35881 actatttcat ctttactctc ctcagcagct cttttcccta cttctggatt tgctcctgct
    35941 ccaagtcctc tagttaattt ttctcctatt tgaactttat attcagcttt tgatgtatat
    36001 aaagcttgtt tatctgtatt tactgatata aattcaacgc cttttagttg agcttctacc
    36061 attctattga cagcattgtt tccaccgcca cctacaccta ttactttaat ttgtgcacat
    36121 tcttctaatt ctacgtcaaa gtttagcatc ataaaccccc ttctctcatg attcaaagtt
    36181 atcgtatgta ttaattatat cattttcttc ttttattttg ttaaccttta agtaacatat
    36241 tccacaaaaa cgtaaatttt cctttttaaa tttcttactt atattgtcaa atattactct
    36301 ttaagtataa aataatttac gtatatatta aatgctcttt aatttttacg ttttttgttc
    36361 ataatattac taataatata tcgtctaatt atagataagt tattaaacaa tctcatacca
    36421 aaaactagta cagcaacata gtaaagagaa actcctaact tttctcctat ataagtcaat
    36481 aatatagcca atatagcatt acctatgaaa ccagtaataa aaattacatt atcatagtta
    36541 tcttcaaaat tggattttat cgctccaaaa atagaatcca ttattgcaag aatagctact
    36601 gacatatata tggaataatc catagaatat gtaaatggaa tatagtaacc tactactaca
    36661 ccaaatgcta atcctcctaa aacccacaac ataaatcttc acctctttat tttactttga
    36721 aacacaacta cattacaatc ctttttatta atttttttta ctttttcttt atttttttgt
    36781 atttttcgtt ttattttgca aataaattca ctttattccc attattttcc tgaactttta
    36841 tattaattcc atatatatcc ttaagagatt tagcatatga ttctggagag tttatagctg
    36901 cactcaataa atctatatct cctatagctt ttattataaa tggttgtcca tatgtagtat
    36961 catttactgt aatagtagct cctgaacatt ttatcttact tgttgctagt actctttccc
    37021 catttataga aattgctctt gctccagcct tttttaaatc atttaaaact ctcaaaatat
    37081 ctatatcatg tataatcaaa tcatttggat tctgtccgtc ttttaactct ctctcactat
    37141 ccctcatagt aatagttatg cctggccctg taacagttga ataaccactt aattctttaa
    37201 cttcttcaag ctctttttgc ataagtgttt ggatagattc atttccattc ttttttgctt
    37261 ctttatattc attcaaagtt tttttgttgt ttttaagagt ttcttttaaa ttttttattt
    37321 ctttttttgt attttctacc tcactttcaa tactttcagt ttgacctaat gttatgtata
    37381 ctctatttgg gtctagtgat tttatataag ttgatatgaa aatgcctaat atgaatgctc
    37441 ctaatataac tattttcttt ttcatactat tatttcccct ctgctttgtt tatatcacta
    37501 tgaccattta tttctatatt gtcttctttg gtcactttta ctttggcatt ataatatgtt
    37561 tctatttccc agacaatacc atactttata ttaagtgccg aagccaatgt atcagaatca
    37621 ccaattgctt ttattataat tggttctttt atgacagtgt cattcataca aagcttgtct
    37681 tcttttaaat acatatatgt attaaaaact attctctggt cgtttataga tatggcactt
    37741 gcttgtgctg aatttaattt atttatcatt gacaataata aatcataatt atatagtata
    37801 ctatcatttt tatcagagtt tcctaagtct tttgtagaat ttatctcaat ttttatgcct
    37861 gttccttcaa catctgtata tccagctaat gtttcatatt tttctatttc tgacttaagt
    37921 atgtcttgtt ctcctttatt gtttttgcct ctataagtct ctatatcttt cttcacacta
    37981 ttaacttctg attgtagatt ctctttttct gtttttaaag atttaagttc ttctgtaagt
    38041 tgctctcctc ttttaaaagt tgtcatccct ctattttcta tattaataat tttaagttgt
    38101 aaactaacaa gaacacccaa tacaacactt cctatgatta tacttttatt tataaatctt
    38161 tttttcaata tatcacttcc ttacaattgt ctatccttct ggactataca ctgccaactt
    38221 tccatagtta agatttatag tccctccatt agtattttta ttttgcaggt caatcagaat
    38281 tttacttacc ctagatatat tataatctaa gttatcatca ttagaaagta agatttttat
    38341 attacttctt gtaagcattt ctatattgct ttcttttttt aagtttacat aatttatttt
    38401 tctataaatt ccttcttctt ttaaataatt taaagtttta aatacattct ccttagtttt
    38461 ataattttta aattttatag atttattatc atcaacgcta taatctacat ctactatcaa
    38521 gtctttttta ctttcattag aacctcttag ttcttccaat aaatttcctt ttttatctat
    38581 ataacaataa ttatcttcat cttttaatac tgcaaatatt tctttttctt ttaaacttat
    38641 aattatctta ttgggaagtt ttctttttat ttccacattt tctatatatg gattttttat
    38701 aagtttattt ttcatatctt taaaattgta tgcaaaaata ttttcattta gatttatatt
    38761 tagttctttc attatatttg atttggtcac cctcttattt ccaattacat ctattttctt
    38821 tacatcaaat aaatcacttt ttacaattat acatatacaa aaagataaca taagtataaa
    38881 aactaaaact atcatgacat tattcgtatt taactttctt ctttttttca aatataatca
    38941 acccctttgg ttaataaata cacctttaga gcttgttttt cctacaaaaa aattataata
    39001 tttaaagtca aatttttgca ttttattaca taacatccaa ataaggtatt aaattttata
    39061 aataaatcaa tatttcatca aaataaacat agtatactac atactattta tatatttaaa
    39121 tctgtataca tacaaataaa cccctaattt ataataattt agaaaatcta atatattata
    39181 aattaggggt aaatatttac ttatcttttc tttattccta ttaccttagc ttgaccttct
    39241 atacttggag tcgtttcttt ttttatttct tttacttcct taataacttt ttcctttttt
    39301 gtttttttag aagttgactt ttgtgttgaa ttgtatactt tcattatctc atcatatata
    39361 aggtctatgg cttcaggctt acctatagtt ttacttgcat ttgccatatc aactaaaagt
    39421 tctctatctc ctaaaagttt aaatactgct gtatttaggc tctctggagt tagatttttt
    39481 tctaaaatag caattcctgc cccttgtttt tctatactct tggcattata ctcttgatga
    39541 ttttcagcag tataagcttt aggtattatt atagatggct ttccaagagc cgttatctca
    39601 gctaatgaaa ttgctcctgc gctacctata accaaatcac ttgctgcaag tgcatttgcc
    39661 atatcttcta agtaaggcac aactttttgg tatggtttaa gatttatatc gctaatactt
    39721 cccataaatt catcataata gcttttgcct gtagcaaata taaaagctat atcttcattt
    39781 accatattct ttattacaag tctcattgca tcatttattt ttcttgaacc tccactacca
    39841 ccatagcaaa gtaccattct tttttcgtca cttattgata gattctttct tgcaatattt
    39901 tttcttgata ataaaatttc ttttctaact ggatttcctg taaaaactaa tttatcttct
    39961 gctgcctctg gaaatctttt atgcgaatct tcaaaacttg taagtacctt tgtaactgtt
    40021 ttagataaaa tcttattagt tacaccagga aaagaatttt gctcatgtat tatggctggt
    40081 attttcccca tagatgcatt aaataaaaca ggtccactaa catatccacc agttcctata
    40141 actatatctg gtttaaattt cttaacaatc tttcttgatt gctccaaacc tttaaaaagc
    40201 ttaaaaactc tttttacatt atcgaaatct atttttcgct taaaaccttg aactgttaca
    40261 gtctttaatt caaatccata cttaggaact atttctgact ctattccctt ttccgtccca
    40321 acaaaaatta tctcagcgtc tggatgctca tctcttattt tgtttgcaat agctatagct
    40381 ggatacacgt gccctccagt tccaccgcct gataataaaa ctttcatcaa atcatcatct
    40441 cctgttaatt aattttgaca tgctttgaga tgtttaacac tattcctaca gctcccataa
    40501 agatggtgag tgaagttcct ccataactta taaatggtag tgctacacct gtagctggca
    40561 ttgatgatgt cgcaactgct atatttaaag ctgcttgtat tccaatttgt gcacctatac
    40621 ctataacaac catacaagca aatacattag aagtcttcaa agctattctt acacatctgt
    40681 agactaaaac tacaaatagc ataataacta ttatacaacc aatcaatcct aactcttctc
    40741 ctattatagc aaatataaag tcattttgtg gctctggtat ataaaaatac ttttgtttac
    40801 tctttcctaa gcctagacca aacaatccac ctgaacctag tgcatataga ccttgtatta
    40861 cctgataccc cttaccaaga gggtcttgaa aaggatctaa aaatgatgta acacgactaa
    40921 gtctataggg ttcagcaatt actaatgcag caaataaagc agccccactc cctatcatag
    40981 caaatacaat tttcatatcc attccagcaa caaatatcat tacaaaagta actagaatta
    41041 cagtacctgc tgttgacaaa tttggctgaa gtattatcaa cgcaaaaaat atccctggaa
    41101 ctacgagaag tggtacaaca ccttttgtca aagattttat tttatcataa tttttttcaa
    41161 ttaattttgc agttaatatt attgtagcaa actttgcgat ttctgcaggt tgaaatgtta
    41221 aagctcctat tcccaaccat ctttttgctc cgtttgcttc tatccctaaa ggggtcaaaa
    41281 ctaacaataa taatactact gctatagctc caattgctgt agcatttttc ttccaaaaac
    41341 tataatctat tcttgatgta ataatcataa ctataaatcc aagtacagca tatattacat
    41401 tcttttttag aaaatagtac gcatcattgt gtttaaatga agattgtata aaacttgcac
    41461 taaacaccat aactattcct acaaatacta aaagcatagt agtgtaaaac actactccat
    41521 caaattcagt cttcatcctt atgtcaattt gttttttcaa attttcttta ggcatccaac
    41581 taatctacct ccattcttcc atttaagtaa gtttagaggt aagagattat tttaaattgt
    41641 ttacattatc tttgaagtct tttcctctta cttcaaaact ttcatacata tcccaacttg
    41701 cacaagctgg tgaaagaagc actatatctc cactttttga aatttcataa gaagtcttaa
    41761 ctgcttcttc catatctttt actatatgta tgtcattaaa tcctttattt ttagcacaat
    41821 tttctatatt tgatgcagtt tctcctaata agaccaatga tttaacatat gactttgcag
    41881 tttcaagaag ttcatcaaaa ttacttccct tatccatccc ccctgctata agtattatag
    41941 gtctatcata agactgtata gcttttatag tggaatctgg attagttccc tttgaatcat
    42001 ttacatatat tacattgtct aaatttctta caaactcttg tctgtgttcc actcctttaa
    42061 atgtcataag tacatccttt ataacttcta aatcaatttt gcagacatat gctattgcta
    42121 tagctgccat acaattttct aagttatgac ctccaggtaa acttacatca tctttattta
    42181 gaaatttgat tttatcatct atatctatta ttatgtcatt attttctaaa tatactcctc
    42241 catttacttt tttagttctt gaaaagtaaa ttactttggc attgcattta tcagctaaag
    42301 attttactac ctcatcatca tagtttaaca cacagaaatc ctcaacagtt tggttcataa
    42361 atatatttgc ctttgcttct atgtattttt ccatagtatg gtgtctattt aagtggtcct
    42421 ctgtaatatt taatattgcg cttactttag gtctaaattc atctatactc tcaagctgaa
    42481 aactgcttag ttcagttact aacacagact cttcacttga agtttctata gtatctatta
    42541 caggatttcc tatattacca actacatatg tatctcgttt tgctcttgaa aaaatttctc
    42601 ctaccaaact agttgtagtt gtctttccat ttgtacctgt aatccctata aaaattggat
    42661 tattcgatag tttaaaagct aattctacct caccaattat atacttgcct gaatttttta
    42721 atttcaatat gaatggtagg tctagtggta cacctggaga cactacaacc atatctatat
    42781 catctacatc ttctgggtga tgtcctaata tgtattttat gtcatttata gatttcaatt
    42841 catcaagtat atttctcaat ttattttcat cttttatatc atttacgatt atacttgcac
    42901 ccaatttatc taaatgttta attgtagata taccagtttt tgcaagtcct actaataaaa
    42961 ctttttttcc ttttaagtcc atttttcatt ccccctagcc ctaaaatact gctattatac
    43021 ttatccaagc taaaacaact gttataatcc aaaaaataaa tacaactctt gtttctggcc
    43081 aaccacactg ttcaaagtgg tggtgtattg gagccatctt aaagaatctc tttcttgttg
    43141 ccttaaaata tccaacttga atcaaaactg atagagcctc cgcaaaataa attcctccaa
    43201 ttataggtat tataagcact gagttagtta gaactgaaaa tgctacaact gcacctccta
    43261 atgccattga acctgtgtcg cccataaaca ctctagccgg atatgaatta aatcctaaga
    43321 aacctaaaca tgctccaaca gttgctgctg ctaaaactgc gacttctgta tttccagcaa
    43381 tagaacttgc aaatagcata aagaatacag aaactattaa tgttactcct gatgctaatc
    43441 catctaatcc atcagtcaag tttacagcat ttactattgc tacgattata aacatcatta
    43501 ttggtatata taatattcca acatttatca caaaatctgt aaatggtatc ataagttgtg
    43561 aagcacttga tgatgatgta tactgataaa aagcaacata aaaagataat gcaacttgaa
    43621 ggattatttt ttgatatgct tttagtccaa gtgatctttt tagctttatt tttataaaat
    43681 catctaaaaa gccaataaaa ccaaaacctg caatacatat aagacctact gccatatcat
    43741 gactaacttt tactcttgta agtccagtta ttagaattgc aacaatcatt ataattccac
    43801 ccatagtagg tgttccgttt ttagctagat gtgtttgggg tccatcatct ctaactgtct
    43861 gaccaaattt aaattttcta agcattggta taaatattgg tcctattata attactatta
    43921 aaaacgcaat caatgcggtg tacgtaagct ctgttattcc taacatcata aaactcctct
    43981 cccttgtcaa ttaactcatt agtttaagct tatttattta gatattcgac tattttttct
    44041 aatctcatgc ctcttgagcc ttttactaaa atagtgtctc ctgtttttac taattcattt
    44101 aacttgttaa acacatcttc tctattctca aagtgatata tattggctga attgaatcct
    44161 aattgttttg cttcttctcc aataaacact gaattttcac ctatagttat tataatatca
    44221 gtattattca tagatgattt tccaactaat ctatgaccat actctgaaat ttcacccatt
    44281 tctagtatat ctccaagtat cgctaccctt ctacctttat atcttccaag tatctttaaa
    44341 gcagcatcca tagaatctgg actagcatta tatgaatcat taattattgt taatttttca
    44401 ttttttatta tgtctagtct catttttgtc cctttaaaat tttttaaacc tttttttata
    44461 taatctaaag aaatatttag acacaaacca actaatatag cagacattgc attatatata
    44521 ttatgttctc ctattgttgg tataaatatt tcctcttttt tcccatttat tacacatata
    44581 aatgttaaac tatcctcttc catagtataa ctttcacaat aaatatcatt gtttttatta
    44641 aagccaaatg tttttaactt atatacatgt tctttttctt ttaatgtaga taaaaactta
    44701 tcatctccat ttacaattaa agtatttgtt tcatcaaagt ttgaagtaat ctccatcttt
    44761 gctttcaaaa taccctcttg tgaacccagt ttttctacat gtgataatcc tatattcgat
    44821 atgactgcaa ttttaggatt tactatatcc actaaatatt ttatttcatt gaatccagac
    44881 atacccatct ctattacagc acactcatgt tctttattta gattaaataa agtcaatgga
    44941 actccaaaat gattatttaa gtttttttca ttttttagta tatttaactt tgcagatatg
    45001 gcagagtaaa tcatatctct agtagtagtt tttcccacac tacctgtaac acctataaat
    45061 ggaatatcaa atttttcttt atagtatttt gaaatatctc ctagagccaa agatgtatct
    45121 tttacctcta ttaaatttat atccgaactt tctaatttta ttccattact ttcatttttt
    45181 ataaaagttt tacatccact atcatatgca ggcttcataa atgtatgccc atctaaattt
    45241 tctccaacta tggctacaaa tgcattttgg gcacttgcct ccctgctatc tataactata
    45301 tcacttacac agtcattata atctccatat attaacttcc cattagttgc aagtactaac
    45361 tcttttattg ttaagcactc cattattaaa ctcctccttt aaaaccttag ctatattctc
    45421 ttagcaacaa aaggcaatgt ataccgttat atatacattg cctacgataa ctcttaactt
    45481 tataattata tactatattt ctgttttttt gaagatgttt atgggtatat gttaattatc
    45541 tgttactttt tagttttcaa aatatagggt tatacttgag tcttcattaa cttttactcc
    45601 aggttttggg aatatatcct ttacctttgt atcttctgga agttctatat ctgcatctaa
    45661 gtctggtttt aacttcgcat cctccaatgc ctttacagca tctcctattt ttagatttct
    45721 tacatctggc actttaactt gcttcttttc atactcagct ttttcttctt ctttatatac
    45781 tggtttaact cccaaatatt ttaaagagtc attcattatt tctttcacta ttggtcctgc
    45841 tgtagtacta ccaaatgcac ttactcctgt tggttcgtca acaatagcaa gaactactat
    45901 ttgcgggtca tcacaaggtg ctattccaac aaatgaacat atatactttc ctggagcata
    45961 cttaccatct ataacttttt gagctgtacc tgttttacca ccaagtctat accctggtat
    46021 atatgctatc tttcctccac cttcagttac aactgattct gcaatttcta acattttttt
    46081 agatgtttct ttagatataa cacttcttac tttttttggt tttactgttt cagttatatt
    46141 tcctttattg tcagtataag attttaccac tcttggttgc attaaatctc caccattagc
    46201 tattgatgat atagctgtta taagttgtat tggagttact gatatagatt gaccaaatga
    46261 tattgtagcc aattcaactg gtcctacatt tttttcgtta tacagtattc ccttagcttc
    46321 tcctggtaaa tctatacctg tcttatccat aaggccaaat gattctatat aatcatacat
    46381 ttttccaaca cctaatctgc ttcctaattc aacaaatact gggttgcaag agttttgtac
    46441 tgcttgttta aattcttgtg ttccatgagg tctgtaatgt ctccaacatt taatctttct
    46501 tcctccaaca gttacactac ctgtacatgt gaatttttct ccatctttaa ttactccctc
    46561 ttcaagagca ctggaagatg taataagctt aaatgtagaa cctggttcat atgtatcact
    46621 tactgctgga tttctccaca tttgataata gccttttatt ttatctttat cattatattt
    46681 ttcaagttct tcttgataat atggatatat tggcgttctt gagtcattag ggtcataatc
    46741 tggttttgat gctaaagcaa gtatatcacc tgttttaggg ttcatagcta ttgcagtaac
    46801 cttttttgca ttatttaact cataagcttt ctgtactgct ttttcagtat agtgttgaat
    46861 tacttcatct atagacaaca caagaccatt accttgtaca ggctcataat atttttccat
    46921 tccttgtggt atttctcttc ctgaggcatc agtacttact ataagttttc caggctttcc
    46981 ttttaacttt ttatcatatt gcatttctac accagatata cctgttgcat ctgaagaagt
    47041 atgtcctaac acatatggcg caaaattccc atatgggtaa tatctttgat tatcttctgc
    47101 tacccatatt ccacttaatt tagcatctct tatttgactt gccttatcat catctatcca
    47161 tctttttact tttacaagag ccatattttt tttgcttatt aaagcgtaaa tatctttata
    47221 atcttcatcc aaaatctcag ctaccttttc agctgcttct tttttatctt ctacttccac
    47281 aggtttacac caaacagtat acttagttac acttactgct aattctttca tatttctatc
    47341 atatatagtt cctcttttgg gttctattgg tatatctctt gtttgttgtt ctagtgcttt
    47401 tgtactcaac caatttccct tcataagttg taggtaccca gtcctaataa caaggcaaaa
    47461 aaacaatgca catgctagaa taagaacgag taccagcctt ttcttactta tcctctttac
    47521 ttttctcaaa tctattcccc ctagttatcc acatctatat actttatttg tccatctttt
    47581 ggatagtcca tattgagcaa ttccttagcc tttagctcaa tttcttttat tgatgttttt
    47641 tcagtttctt ttgcctttag ttcctctaat actatctctt tttttcttaa atctttttgt
    47701 aaataataga tatcgtactt catttccgag atttttgaat aaccacatag attcaatatt
    47761 attactgaaa aaattgcaat cagtaaaatg catttagctc ctattttttt cttatttctc
    47821 tttttataat tattcttttt tttattttta tattcattta gattatgtat tttataattt
    47881 tttttcaaat tctcaccccg atagtatata gtttaaattt tcctctatac tatatttgtt
    47941 attaatcata tttcttcctc taaaatttca tcgctaaatt aatacactat tacacaatct
    48001 aattgtattt ttttctatac acaacaaaaa cctataaatt cacttatact tttatatgaa
    48061 tctataggtt agatattaat ttctatttat ttttatcact tctagtattt ttagataatt
    48121 tcttccataa atataatatg taactaacta tctactctct gattcctaag ttctccatta
    48181 tattacttaa taccatctat atctatattt ttatatctaa attttcatta tatatctcaa
    48241 taacaaagaa catttcttat agtgttaatt gttttttaat tgctttacca gttcaacaaa
    48301 atatataata ctttttaatt aattatagta atcttatttt atatataaga ttattcttta
    48361 gccttataac tacttatatt ttttctgcta ctcttagctt agcacttctt gaacgtggat
    48421 ttacttctat ttcttcctca ctaggtaata taggttttct agttattatt ttcacctcag
    48481 attctttatc acattggcat ataggtaaat gttgtggaca tatacaatct gatgctaaat
    48541 gcttaaatgc atttttcact attctatctt ctaatgaatg gaatgttatt atacatattc
    48601 ttccaccttt attcattata gaagaagcat catttatcat ctttgtaatt actccaagtt
    48661 cattatttac ttctattcgt attgcctgaa aagttctttt agctgggtga ggtccatcta
    48721 ttctagcttt cttagggata gcttttttaa ttatatctac taattttcct gtagtttcta
    48781 ttggtttgtt tgctctttct tcaactataa atttagctat acgcttagcc cagttatctt
    48841 ctccataatc ttttattatc ttagttaatt catcttctgt ataagtattt acaacatcat
    48901 atgcagaaaa ctcacaccta acatccattc tcatatcaag tggtgcatct tgcatataag
    48961 aaaatcctct atcagcttca tcaagttgat gagatgaaac tcctaaatca gctaatacac
    49021 catcaatttt gtaaactcct attttttcta attcctcttt tatattttca aaattgctgt
    49081 gaacaaactt cactctactt tcatattcac tcaatctctc ttttgctgtg cttatagcat
    49141 ttttatcttg gtcaaaccct ataaataatc ccttatctga cagttttttt actatttctt
    49201 tagaatgtcc tgctcctccc atagtacagt caacatacac gccatctggc tttatattta
    49261 aattctctat acactcattt aaaagaactg atacatgatg aaattccata gttacctccc
    49321 ttaaattcac tttttaaact attcttcact aatattatgt ttatttttct ttgataaata
    49381 aacatgtgca attatgccac caataactcc aattagactt ataacttgtg ccattctaag
    49441 tggtcctaac attaaactat ctgttcttaa tccctctata aaaaatcttc ctattgaata
    49501 taaagttata taagtaacta taacttgacc ttcatatttt ttattttttc taaatagtaa
    49561 taaaactata aatattccaa aatcccatat ggactcatat aaaaaagttg gatgtacctt
    49621 tactccatca accattattc cccaaggtaa attggtagca cctccatgtg cttctccatt
    49681 aataaaattt ccccatcttc caatagcttg tgctaaaggc attccaagta taactgtatc
    49741 agccattttt aagaagttaa tatttttaat cttagtgtat atataaccag ctaatattcc
    49801 accaattaac cctccatgta tagcaagtcc accacctcta aaattaaaaa tttgagacat
    49861 attttgagca tagtaactcc aattaaatac aacataatag attcttgcac agattagtcc
    49921 aactggaatt gctattatag ctatatttaa tacatcatca tctttaattc ccactctttt
    49981 tgcttctttt attgctatta aagttcctaa aatcattcca catgccataa gtattccata
    50041 ccacatgatg tctatgccaa acagtgtaaa tgctactcta tccataacgt cacctctttg
    50101 tattaattga aaatatttcc aaagcaatat gctataatta caattaattg taattatact
    50161 aaattatgat aacacattaa tgttaattct acaaaaaata ttaaaattta ttctacaaat
    50221 ccctacacta tattatacta atataattac tataaaattt tttaaactga atctctcatg
    50281 actaaagtta caagtgtaca tagatatttt ttaatcaaaa agattgatgc atatatacat
    50341 caacctcttc tattccttat ctatagctct taaattttta cctcaattta tataaatata
    50401 ttttataaat ttgttttacc aaaataaaca tttaaaaaca taaataatac ttaattagtt
    50461 attcttatta tgattaagtt tctatttagg ttctacaata gaaataacac aatactcttc
    50521 tttaaaatgc tcttttaatc tttcttcaac ttcctcaaaa gtaatatctt taataacatc
    50581 taaataatcc aatagattta tatccttaaa aacataagat ataaaactat tacctataaa
    50641 gtttatagaa tcaaagcact taataaatga ccctattttt ttctttttag ttctctcaaa
    50701 ctcttctttt gataatcctt ctttttttga tttttctata taatcaagaa ttatttcttt
    50761 tactttttta ggctctttag aatctccagc tattatgcta aaagcataat ccacttgaga
    50821 cgaaaatcca gctccaaaat tttcatttat taaaccttgc atatacaaat cttcatagag
    50881 cttacttcct tttttaaata acatccctac aagtatatca gttacaatct ctttacgtaa
    50941 aagttctttc ccttttaaac caacattact atctttaaaa cctatgttga acataggcat
    51001 agatataggg aatttttcta taacctcttt ttcttttaca ctttctggtt cttctggata
    51061 aaatctttct atactcttag agagtttgtc tactttgtaa ttgtttgact ttttagttac
    51121 atctataact ttttctacat ctaagtcacc aacaacaaat aaagccatat ttcctggatt
    51181 atagaaagta ttataacatt tatatagctc ttccttagtt atcttatata tactatcaac
    51241 agtacctgct atatcaattc tagcaggata atttacatac attgccttca agcaattaaa
    51301 gtatacattc caatcagggt catcattgta catttttatc tcttgtgcta ttataccttt
    51361 ttccttttca acattttcat ctgtaaaata aggtgtttga acatagtcaa ttagatgttc
    51421 taaactctca taaaaatttt cagtagcaga aaataagtag gctgtcattg taaaattagt
    51481 aaatgcattt gcatttactc ctaattttga aaatttatca aatgcatctc ctccatcagg
    51541 ttgctcaaac atcttatgct ctagaaaatg tgcaatacct tcattcactc taattttctt
    51601 atcttctcca attggtacaa attctaagtc atttgaacca taattagtag ccaaaatagc
    51661 atactttttc ataaaacccc ttttaggcat aaaatataca tctaatccat tttgcaattt
    51721 ctcataataa acttcttctt tcaatatatc attaactatt ttttccatag acatctcctc
    51781 cattaatttc tcaagaagta tacagtatca agctctatat tttttatagc ttcaactata
    51841 tctttttttg taaccttttc tatactagat attatgtctt gtacttcaga atttgtcttt
    51901 gccatagact gagagaaaac aaaatcagac ataccaccta tattatcagt tatagatttc
    51961 atagaattaa ctaaagcaag tttactgttt tctaattctt catcagatat atcacctgct
    52021 tttacacttt ctacttgttt ttttatcaat tctacagctt tatcataatc tttagtttct
    52081 ataccagatg atataaacat gatactttta tacttctcta ttgaagagaa tatatagtaa
    52141 cataaacttt ctttttctct tacattaaca aataatttag agtgtggacc accacctaag
    52201 acattgctcc caactactaa agcataatat ttatctatgt ctgcataatc cacatttgct
    52261 ctataaccca taactaactt accttgagtt atttccattt gttcatctat aacttttacc
    52321 tcatccacat tttttataaa atctgctctt ggtatattta taatttcttc tctttcaaat
    52381 tttagatttt tacttattat atctacaact ttatcttcat caaaattacc ttctacaact
    52441 atatctatag gtgatgtttt aagtatgttt ttataatgtt catataactc tcttgaagtt
    52501 atactatcta tttcatcttc ataaccaaat tcacttacac tatatctctc tcctttacac
    52561 atagcctcaa tacatttatc ttgcgcatat tcctttttat catttattat tgattgtatc
    52621 ttttctctta aattctcttt ttcaatattt aaatattctt ctttaaaccc accatctaca
    52681 acaagcgtat tatttattac ttcattaaaa aattctacta cttcttcaaa tatactctca
    52741 tctaaatact tttcacttat gttaattact ttaaaactca atacttgacg ttctccacgt
    52801 tttacagcat cagctcccat agatgaacca tataaatcat ctaatttatt ggatattgct
    52861 ctagcactag gatatttagc gcttccactt gctattattg atggtattag agcatttttt
    52921 gtagtttctt tttcatccaa tagtctctga acatataaac ttataagatt cgatttaaac
    52981 ttttctgttt ttatcaaagt aagattaata ttattaccta aatttattat ttttttagta
    53041 ttaaccatat gtacctccta tttttattta aacatcaa
//
LOCUS       DAMJVX010000011        51925 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMJVX010000011 DAMJVX010000000
VERSION     DAMJVX010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51925
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.012"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            283..738
                     /gene="smpB"
                     /locus_tag="PC407_000582"
     CDS             283..738
                     /gene="smpB"
                     /locus_tag="PC407_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDF2496062.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            896..1245
                     /gene="ssrA"
                     /locus_tag="PC407_000583"
     tmRNA           896..1245
                     /gene="ssrA"
                     /locus_tag="PC407_000583"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1919..2824)
                     /locus_tag="PC407_000584"
     CDS             complement(1919..2824)
                     /locus_tag="PC407_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HDF2496063.1"
                     /translation="MESKEKSILRATHFGIIKIGEKDLNCAVLENGSRIINKSTVFKA
                     FGRTKRGRKKDEIRVPSKPNLPSFIDAKNLSDYISDELEDMLINTIKYKTKNGRIVEG
                     YPAEIIPLICDVYLQARLDNKLAVQQEGLAIASEILVRSLSKIGIVALVDEATGYQYD
                     RDRDELQKILSMYISKEFLPWTKRFPDEFYRQMFRLKNWTYPRPSSKRPGIVGTYTNK
                     YVYDLLPPGVKEELQRVNPTVKPGQRKHKHHQFLTEDIGNDHLKNHLLKVITLMQASK
                     DWKDFNVLFNRAFNLPVQLEIEYDE"
     gene            3735..3950
                     /locus_tag="PC407_000585"
     CDS             3735..3950
                     /locus_tag="PC407_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HDF2496064.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKSYIVEDLIQVLEEKGVM"
     gene            3950..4429
                     /locus_tag="PC407_000586"
     CDS             3950..4429
                     /locus_tag="PC407_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HDF2496065.1"
                     /translation="MIVYHDSYCVAKTPNIIFLRDSLDFGKGFYVTSIKTQAINWIER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFNSYNLEWLDFILECRNMSDVYMKYDVIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRCEEEV"
     gene            4435..4665
                     /locus_tag="PC407_000587"
     CDS             4435..4665
                     /locus_tag="PC407_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496066.1"
                     /translation="MEANKTLLQILYKKIILEFSNQTGKSLEESMDYLYTSETYKLIS
                     EGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(4781..5947)
                     /locus_tag="PC407_000588"
     CDS             complement(4781..5947)
                     /locus_tag="PC407_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2496067.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHMQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENRLEILTLEQQKNFIKAIEGHKLEVLFLVALGTGLRLGELLGLKW
                     FDIDFKNSNLTVKRTLQRTYFIDKNGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNVDILKAGELYRNDNYVFCNELGVPIDDKRPLRNLKSILSSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(5937..6689)
                     /locus_tag="PC407_000589"
     CDS             complement(5937..6689)
                     /locus_tag="PC407_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2496068.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYIIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNNLFISDISIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            complement(6781..7272)
                     /locus_tag="PC407_000590"
     CDS             complement(6781..7272)
                     /locus_tag="PC407_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2496069.1"
                     /translation="MINNTIGEKIKQYRKSINMSRNELANKIGCSVHAIAKYEQNQRI
                     PSLDMIRKISTALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVVHILENYGYDI
                     KTDYKKKFEITDNADTIKPISLSFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            complement(7269..7466)
                     /locus_tag="PC407_000591"
     CDS             complement(7269..7466)
                     /locus_tag="PC407_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496070.1"
                     /translation="MNKAYMLKYTYKYPTNLFYGDKKYTLTYIKRIMSKLVYDIDNLE
                     NNSFICVKIQSLFDDIFKVEL"
     gene            complement(7523..8287)
                     /locus_tag="PC407_000592"
     CDS             complement(7523..8287)
                     /locus_tag="PC407_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2496071.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYFLQSLFPEEYYSFDEFLNAISNLME
                     DDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCVSNTELDFSLHPDDI
                     KEIIKNDVLIKQSIDELLDIGLSEENIEAIYDYFSEATDNGSNIRLKKLISQKENMTI
                     KNNDYFKLLQENNELKEKNKKLENTLKEIKKLLTKI"
     gene            8434..8634
                     /locus_tag="PC407_000593"
     CDS             8434..8634
                     /locus_tag="PC407_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2496072.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKEE"
     gene            8638..8952
                     /locus_tag="PC407_000594"
     CDS             8638..8952
                     /locus_tag="PC407_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496073.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            8974..9717
                     /locus_tag="PC407_000595"
     CDS             8974..9717
                     /locus_tag="PC407_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496074.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKRIKIKSGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSIIREYDKEAVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYHSKEIVINKETGIYNPDDSQLKW"
     gene            9730..11064
                     /locus_tag="PC407_000596"
     CDS             9730..11064
                     /locus_tag="PC407_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2496075.1"
                     /translation="MNNSNNLIKNTEAEQSILGSILLDSTIVEELEQMLNEDMFYHEE
                     HKYIFNAIKEINKRNEVIDIITLTEELKRKGHLNNIGGISYITSLSTIVPTVSNFTSY
                     VSIVIEKYKNRNIIDKFNKFNLGEIDDNTLIYQLENDINKANQKLIKKDSSITAISER
                     AFEYINDEMNEGIKTGIKFLDDTIGGLYGGELTTIAAKSGRGKTALALQILRNVIFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNDDEIVKYIQALSIINQENNLY
                     INDSVNTVSGIKREIKSTKPSLVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPVIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALNYCKTMGIE
                     DIRELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGNLFSFEECKKIQEI"
     gene            11314..11586
                     /locus_tag="PC407_000597"
     CDS             11314..11586
                     /locus_tag="PC407_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496076.1"
                     /translation="MYDEIIEIYFDYWINEHMYRSILEKDEYYIECWKEVKKINELLE
                     INLEDDKKYLLVELNDLFNSLIEFERELSYKLGFKTGAKFIIELLK"
     gene            11859..12986
                     /locus_tag="PC407_000598"
     CDS             11859..12986
                     /locus_tag="PC407_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDF2496077.1"
                     /translation="MIIPKLEPNDRKREFNKYSSEQINKVVYNYLFKNNMSNRKIDDE
                     ILDLSSKQSKGYQSMGIVHHLGINKNHKGIFTGYTIDKAIEVLKEDEIHFKQIINILL
                     EIRNINEYPIIVDSWELLDEKIALKTIDKSTLKHNGTGVPIEIRYFFEINNLGKNEKK
                     NVNFKYNDNYYSAVIEMDKLENPRSRIIWKSKFRDLLRSIFSEYYEKFLENKIEGKTN
                     LPKIRFIKLDSNNFNIEFIFTAEIEDDVLEEISQDNPLNINTINIELETEIRKEGKVK
                     YVYGKQYERNPRNRIEAIKYHGTKCIVCGFDFEKTYGDRGKGYIEIHHIKPLSSVGEE
                     SNINPKTDLVPICSNCHRMIHRKKDNVLSIDDLKQIIKDSK"
     gene            13466..14113
                     /gene="cas5"
                     /locus_tag="PC407_000599"
     CDS             13466..14113
                     /gene="cas5"
                     /locus_tag="PC407_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HDF2496078.1"
                     /translation="MILNKIEIIGDMAHFKIPMHSKIQRTYEIPPISTVVGILKNIYG
                     EEIDDFILGYVIYHDGTFKDLQTIYKEINPNVKTLTDSDRFQTDVCPIEYLINPKLII
                     YTNIDKNIEFNEPLCLGKTNCLAKVLSLSEIEVNLIDKKAIGYNQYTDINIGDGMIKR
                     INTLTEYNEKKGYYDIYSSMVRENDEFEYGKYYDEDEQQNIFLWKWKKGGVIKCI"
     gene            14104..14868
                     /gene="cas6"
                     /locus_tag="PC407_000600"
     CDS             14104..14868
                     /gene="cas6"
                     /locus_tag="PC407_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2496079.1"
                     /translation="MHLVRRHLILTVDNEVVLDYNYQYELMKRIYEAIEINDKRKALS
                     LHNEGYKVDKKVFKLFNYTIMFENAKYLKEGIHLNPQTKIKLILSGYDDILNNIIKGF
                     IKCKVFKLNNIEFKVSDIEEDSKKNFNNITLYKVRSPIVASLYDLKSRKQVYLNPMQE
                     EFYKALHDNLGNKYKLIHNKEYTGELYFDIEDVLAVKKKYITNIKGKGFIIGYTDFEI
                     FVQANKDMQEVIYYCGLGEKNSIGMGLLTYITSRRA"
     gene            14870..16036
                     /locus_tag="PC407_000601"
     CDS             14870..16036
                     /locus_tag="PC407_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496080.1"
                     /translation="MDRSYNVFVDNGLYVLAYYLNKDINDITYQDIENSIDLMSDKIE
                     EFVSCEKYSNLKSMCFSNSTLTQPKGKVTLNEKLKGFVKNQGDEYCSLCGQYKAKVKI
                     EDKEYNIGRSYMPNLVANTFYNFSNNLQGLNVCPYCLVLTMYSILNCRVSRYAFLYNS
                     TSSEFMEDYTFHIQKENLTDVELGAKKEKEKYSIVKLLESLVCKYNLFNGDIEQYMFN
                     NSGQSQDIQVNRIKNKYVNLLIKLQEKALLSNFEKLHLDRYILNGTLESNYLREVYRV
                     KKEEKMDEKEQKNFDELVIFLNREVNVLKKVKIDIIKKLTDKLKTSDLDIDKIYKELK
                     MLGKFYAYEDWLVNLNEMYYEKTGEKLFEAEDYIELDDIRSFRQIKNLILVSLI"
     gene            16057..17127
                     /locus_tag="PC407_000602"
     CDS             16057..17127
                     /locus_tag="PC407_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HDF2496081.1"
                     /translation="MENKNSNRMLNIGYAIEVLCGRLNGSSSEVEKKNNSYKNSTYTK
                     KIGSRSRISSQCQKYNQKLFMEQVCGYEQNVKTKDGKQVKLVPNPFKYATDDIFGFMM
                     AEKLSIEEDVYKELSDEEKEIYKKNKKKYEVNITKKRKSRWQMNSLINVSNRKVEWEW
                     NVCGTTGDSMPYQVEVYSGVHTNIANLNIGKIGEYDISDIASEHRDYTTLEAKSIGVE
                     KLDKEEKFKRIEALLKSLEYLSVEGNKTNHLTDTKPKFVILGEYSWGNNVFQGIIKEN
                     GLDVEMLKESIEQNEEFRKSDIWIGINKFYDDTFNEIEVNNEKEQIKKYLEEEFEEYD
                     FMKISNVHQAFESYLKYLKETL"
     gene            17138..19123
                     /locus_tag="PC407_000603"
     CDS             17138..19123
                     /locus_tag="PC407_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2496082.1"
                     /translation="MNNLYEYKAKSDGTSIDKHNEDMNYIMNQLVSIYNISNDMKNDL
                     YEAIVCHDVGKVVKSFQDNIESSTRRVRHELLSASIKYLNRGQKLAILTHHKDIKFLE
                     GQITNSIYEIELKELKELLDIEVEDIRPFIKEISRARNDDTRKLENILLKGYLQYCDH
                     IASAGIKSIDKGFNSIKDFSIPKGVSYNSIQEKVLNMDKNEDIMIMAMTGLGKTATSL
                     YWSNLVQNEYKSKRVYYILPYTASINSLYENLYNRNISVSMLHSKAPYFLNKISDEED
                     IKQLYQIYKKSVKQINVCTIFQLVKSVFSCKRFEMLLSQLKDSIFIVDEIHCFDTRVL
                     CYTLEFLKYLKENLNVKICIMSASIPTCLKKLIQERLQISTIIEPDSKDLIFRHRLNR
                     LSEGILDSIHKIENDLMQGKRVLICVNSVDLSQELYGRLQKYNPKLIHGRFNTRDREQ
                     AEKGIEESFLLIGTQSIEVSLDISYDVLYTELAPLDSLLQRFGRVNRRGDNAELSNIY
                     IYDYDRKLNIYDEDIINRTDIVISDIIHNDNSIVDESKVPSYLDNVYLDIDMDKYNKH
                     KVALEIIMKNMRLGVWNKSANNDMLNNDTISVVPECLLEEYKLYIDKKMYLEASSLFV
                     NVSVKRYNYNKKMFYECGDIVVTSYSYNDKLGLIFDC"
     repeat_region   19376..19998
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=19376..19404
                     /rpt_unit_seq="gtttaaattacactaagttagttataaat"
     gene            20443..21999
                     /locus_tag="PC407_000604"
                     /pseudo
     CDS             20443..21999
                     /locus_tag="PC407_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444896.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            23177..24391
                     /locus_tag="PC407_000605"
     CDS             23177..24391
                     /locus_tag="PC407_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HDF2496083.1"
                     /translation="MGEKMQSVICKIFDNKLLISNDLSEQDDVLYEVMDKFIDDIEYI
                     SKLGDEQIKSMLMEVNKFEEDSTEFELYMKNLCYLKQSYFIDTNDKSEINSIDIDILK
                     NVKIEDLVDITNIKLERNLNINIEHISKAVNKIYEENEKWKEYNIQNTMSKYIGIPES
                     EVIFISNLSQYVSFLSELDNAGDYVSRGQKDCMYELLPSLHRKYKKDYGIHSDEYEEA
                     FKQKIIYYDKEIKNRSSEELRAEGQHFGLPTEYLDFTEAHLISLLFAIEDYKYTKQHS
                     IVYFIDSISYNTDTIKRSEKLVNYSDTSAIDNCSKFSSRSFFIKVGNSNERIHFQKGC
                     FLKVSSEDKGEFKEKLKKYCKVVVINKDAKKEILKELFNLGITFENIYPDKDNVVKSI
                     KFYYDEIVGGNL"
     gene            24391..25683
                     /locus_tag="PC407_000606"
     CDS             24391..25683
                     /locus_tag="PC407_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDF2496084.1"
                     /translation="MREQFKNSIKNIKSNRPLVSVQDIVNFIDKGEKYLKLSIDEIDE
                     MEILQGIVMSPDYQRTYKSSNKEESSIIESLLIGIPIPEIFLVKIGVNDMQIRHVMDG
                     QHRLNAIYRYVKNKYPLKNLDILGNDPLYDNKRFSELEKKDKIKILGSHLSILEFDGF
                     DNPEIEIELFKRYNRNTKPLEMQEIEMATYFSETSKYISKFINTLIKDKYKDEIDKSE
                     YKYANYKSDLLRIYNITKSRNDKQKNHQEICIIFSILENGLQENIRDGVTASKKFLEY
                     KSIQYKNNESEKLELLKDKFDEFNRMILKISEKIEYPFSTSLISDDKQRQSKYLIGVS
                     IVLASICYYYDIDLENNYLIQDIKKIISCSPIADIEYKASSTNMKNMILYLFIKNKIQ
                     DNKFESLNLKRDKIKEINTLVMNNDGYLNEQTRLLEIY"
     gene            complement(25910..26095)
                     /locus_tag="PC407_000607"
     CDS             complement(25910..26095)
                     /locus_tag="PC407_000607"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2496085.1"
                     /translation="MEGFWRILKVEMYYLRKLDTYEQLVKTIEDYIYYYNNFRYQKRL
                     NSMSPLEFRKYLNTEIA"
     gene            complement(26125..26514)
                     /locus_tag="PC407_000608"
     CDS             complement(26125..26514)
                     /locus_tag="PC407_000608"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDF2496086.1"
                     /translation="MRAVGLSSVCRKKKFSYVKCTPEVIAENVLSRKFSADKTSQKWL
                     TGVTEFKLTNGMKAYLSAILDLDDRSIVSYVIGKSNNNNLVLETFDKAIELYPNAKPI
                     FHNDKSFQYTSKVFKSKLLTQGMIQSM"
     gene            complement(26526..26986)
                     /locus_tag="PC407_000609"
                     /pseudo
     CDS             complement(26526..26986)
                     /locus_tag="PC407_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            27415..28425
                     /locus_tag="PC407_000610"
     CDS             27415..28425
                     /locus_tag="PC407_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496087.1"
                     /translation="MITKIKKTYIYLKEIQKIYKINEHKELVHIIKKLINDNKIKPIK
                     TSDLTPQHEQLYTKYRIIKQETEEDKKILEEINYKICDKLSIDYYRKNLVHYKNNRVA
                     IMDLNSYLIKKSDNLSKKISINERSYEIFKDEKFISSKVGKEVLKNLKIDLIKDLNVY
                     KTPEPFIYLSINRISPQKILIIENKDTYITITKMLLEGKEILKNKIDTVIYGEGKKII
                     NSIKGLKEDITIEYLTNKENEFLYWGDIDKTGLSIYTSLKDTNEINNIRLFEVAYEHM
                     IDKTKENTLRNIPKNQKDNFECGLENINNLNLKESIKNILVQGKYIPQEALNRFDLEE
                     YN"
     gene            28425..30044
                     /locus_tag="PC407_000611"
     CDS             28425..30044
                     /locus_tag="PC407_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496088.1"
                     /translation="MEMINILEGYNKRVNCIGSYYLIATLANNRNKFKEFDNIQFYNL
                     LIQVLCYIFDRSLRRKNCLRDDIKDFIEEINRMDYKIMLSEDDLKDLANYIINGLTNS
                     GKVYLFTYYSLEQEKHIDESIKIIEDKNVKINNQERLSYSLTTEGYRLLLSTKEYDEL
                     FQIQISQMIAKLRIEKGDYQGAKEDIYDIINTLEIHQQKIDNYIKIIKVNILYIKEEK
                     YSTVIDTTVETLLEEMDKYEELKKEVKLLIKDKEDYLEKDEDIDKEKIKNIRTQLLQM
                     KELIDGIVLAKSTATNLLSRVQDFREEFKEILKKLLTTPVIKRFNFKEEILDRLEKDS
                     DFLLIIRELYISLFKVELPNRFNIGVAYQEQKLLNTREIENEIVNKDELEIIENKEKE
                     ERINYCENYYFDFFKYLIKYGLEKGQFTLEEFLKNYLKYDKVIYLEITKDSKLMRDII
                     IYFANQRESINIKKIIRMLENSAFSPNTDFQIERLIERLLVEDFELLNSVSHIKSNKI
                     FEDEMKINTEINLETMTAVKLEIANIKFEII"
     gene            30062..30790
                     /locus_tag="PC407_000612"
     CDS             30062..30790
                     /locus_tag="PC407_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496089.1"
                     /translation="MNISLNDYKIANEIFYRLLKKSELKEKEAKELFLAYINNSNVRY
                     ALEAICEVSKIKIILISDTLYLIPNNDNDIFGFDYRRESLLGKDLKDSYLGYLIMTII
                     FSEFTNGVAPASYIQVLNLLDLVQESLERSIARDNLEEIEKSCEFNITILKNYWESKR
                     KWDESSKKGIETTSADFQIGFIRKIINFLKNEGLIVHIEDEDKIKPTRRFLDLMNNYF
                     LDEERKVLIEKLVLRKGESINASN"
     gene            30777..35132
                     /locus_tag="PC407_000613"
     CDS             30777..35132
                     /locus_tag="PC407_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496090.1"
                     /translation="MPVINKYKIINFKFGMGQERILSNRTMELYGENLQVEAENTAGK
                     TMSIQTIIQSICPLSDVAKPFTAIYNKKEPLYSMTEWLLDDGKTLLLTGIGFEKKAGS
                     SKEEGKEKKDDLYKYFMFIIEESKEMEVDIDNIPLLKNNDKGIRIVQPLTASQDYIKM
                     LRDKYGKNKVHFFNNYLRKQHREKLEEYGINQSEWKEIIIKLNSNNKEGGLSDFVKEH
                     NTSEKLIRDKILPLIETSLTTEEFAPILQIKEQVRKCINNIIDMNEKLSDYKNYRDLN
                     IRVESLQNKINRILFIEEDKNNVLGELANLYVHIENKRNELNEEIKQYQKMKASKEEE
                     FKIVEYEEDSYEYFRKEKEIQDTQVEFDKAKNELNDKIEELELDEQYLNISKFKRKKK
                     GLEEGEKDKIEKESEKQRKEVNQEEITAIINRLGSSIHYCLKEESIINNEKLKENTNN
                     NIAIQEKKNQLNDDKSKLTKVLISKNSEIKYIEGKERDFNNQLHEFKLNNTDIKGFIS
                     NNFLGEVIDYTRYINYLNIKVKSIEENLESKVMQIEESNKLKDDINEAINNLKLDNHE
                     SYMKLQVLKTRKKDIDNFKEKYKIIKTRFEIDNELDNSTKLYKEVKQELNKMKLQVQD
                     LENNKKQLEKELEDLQNYKDIIFEEEILTDLERNGIKPIEGMKYLLTLDGDLEYKNNL
                     ISKNPLLPYSIIITNKEYQKLKQINLKNKTKISMPIFVRDNIEDTFITTSSNLVISNG
                     LNILSSFDNKALDPEKREKDMNLIKEQINSIRDKVEQYKAEIEILEEFKLLTNKIDFT
                     AEDLSIDVKIENYKKIIFEINNKIKSNENNEKNLSNKIKELYENKEKLLNNKNILFNK
                     LKDIRNIQDTYSIILENKNEITKLKTSIEKIEEEKKVIKKSIEDIDVNIFNLNSEKDR
                     INRHIDDTESKINKFKIYEPIKLENAKLEELEIKYKKYLEDPTKKEIETLNYEISVCL
                     KRIESITIELEEFRQLISSDNFENIPILFTIEELNKRIVLNKSKVDELRKLKYSIKSD
                     IDKNQGSLNTKREEILKKFNGKEPLSTSLIVNFNFEERKIDIKKDIKDIGEIIEGNRT
                     TCQNLEQELRDLKKYKVERKAITYQPEDIFIESKKLQSKLQNVEYNLIKSINSYKNTI
                     LEIKEFNYKHMEKYKNFIETLNVNKDNYSRQKEQVEIVLLVIREEISKLEKHSEQLRK
                     EKTIISRQIKDYVMDCIDEFKIINKLGKHKGQALFNISLPKSDKIENSLSLVDMLMDE
                     IVEEANLGDVEQKINTFYILNKTLNIGRIPVSVIKYEINRNETIKWNSINNETTGGQR
                     FCISFIVTILLMEYKRYDRNAIVDESKYKGKVLLMDNPFGETSQQDFLKEIFELAKKF
                     NVQIISYTHVTNASIRAMFNKIYLMTVEKTTSNKEFVDINEIKQNKTDELVYMSKFYI
                     GNKEELIQEDIFSLI"
     gene            35234..35443
                     /locus_tag="PC407_000614"
     CDS             35234..35443
                     /locus_tag="PC407_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496091.1"
                     /translation="MKNFVIVSTKAALLRVIIVRLLLKIGLKTYKYLSYFMKKEKLKR
                     IIRPEGCELRVNRSIQVERAIAEQI"
     gene            35839..35979
                     /locus_tag="PC407_000615"
     CDS             35839..35979
                     /locus_tag="PC407_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2496092.1"
                     /translation="MKIKIKKYREKLLMIQNELAKSVGVRRETIVHLENGLMYHKGIQ
                     DK"
     gene            36117..37079
                     /locus_tag="PC407_000616"
     CDS             36117..37079
                     /locus_tag="PC407_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2496093.1"
                     /translation="MAKIDNMMSILWMLNSDKKVTAKQISEKLEINIRTVYRHIDALS
                     ASGVPIISDTGHNGGYTLLNNFIKAPLFFDTEEQTALLHAAIFAKEEGSFLDDTLDKA
                     TSKLKMYSNQKQEETLTKHLIGFEVIKPTGKISIESVLKKLKNSIANELSVEINYRTA
                     HEEKSKCRIINPYGIVRWNNNWYIVAFCQMRNEIRSFRVDRISSVIETSNTFNRPKEF
                     SASEFFIRGIMDETKDEDNLISLVIEGKMNALDNLCQHWLLGYYLKERTSNKAIFKLQ
                     EEIIFTYVPNILIPYRKSIQIIEPLSLRDKMVEDLLELINHYQI"
     gene            37157..37786
                     /locus_tag="PC407_000617"
     CDS             37157..37786
                     /locus_tag="PC407_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDF2496094.1"
                     /translation="MKKRKVYMYVFDTMSDWEVGYLTAELNTGRYFKKGLKPLKVITV
                     GVDKNPIITMGGLKVLPEITIDELNIESNDLLILPGGNTWTSIGHEKILEKSKEALEQ
                     RSIVAAICGATLGLAKTGLLDFRNHTSNDLEFLKMVILSYSGEKYYKMESAVNDENLI
                     TASGIAPLEFTVQVLKVLDVFKEEALSSWLNLYKTHDSKYFFELMNCNK"
     gene            38617..39033
                     /locus_tag="PC407_000618"
     CDS             38617..39033
                     /locus_tag="PC407_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496095.1"
                     /translation="MSKSETGVKSKLILELLVITYLFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSKFILSKVNQIAITIMMYSIVIIAIFFATPYISNI
                     NISNLDTGIILIAVLFIQSLSKLILYIYFDKRGICN"
     gene            39034..39246
                     /locus_tag="PC407_000619"
     CDS             39034..39246
                     /locus_tag="PC407_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2496096.1"
                     /translation="MAISMKTKIKEYREKLLMTQNELAKSVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFGTTVENLFEFIEED"
     gene            39881..40924
                     /locus_tag="PC407_000620"
     CDS             39881..40924
                     /locus_tag="PC407_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDF2496097.1"
                     /translation="MKRKIAVVLLTVAMLLSLVACSNDNTSKSDKLEDTLVVYTTHGE
                     DMLGAISEEFTKETGVNVEFINLKGELADRVRSEKDNPQADIMFGGDTSTYMQLKEEG
                     LYEKTEPSWNNELDKSYKDSDGYWYGTIKTPVMMFYNTKLLSADKAPKDWSDLTKEEY
                     KDLIVTRDSLSSSMRSTISNLINYYSNTKSEEEAWKYLEDLNKNIKNYYNSGSVMYSA
                     IGKGEATISVAVLSDIIENKEKNNMPLEVVDAESGSVVITDCVAALKNAPHPNAAAKF
                     VDFVGSAKVQAMVANDFNRIPTLDSALKDSPKWMSKEYKAMDVDWSVIGKNQSNWIEK
                     WETDIIDNNKTIK"
     gene            41185..42059
                     /locus_tag="PC407_000621"
                     /pseudo
     CDS             41185..42059
                     /locus_tag="PC407_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            42078..43070
                     /locus_tag="PC407_000622"
     CDS             42078..43070
                     /locus_tag="PC407_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2496098.1"
                     /translation="MKNIELKEINHYYGKEKVLHDINISIKEGEFFTLLGPSGCGKTT
                     ILRIIGGFIKPSSGSIYVGDKNITNLEPENRNMGTVFQNYALFPNMTVEENVSYGLKI
                     KKLSKKIIKEKCDNYLELSGMKDFRKKKIDELSGGQQQRVAVARSLATEPTMLLLDEP
                     MSNLDIALRIKMREEIREIQQKTGITTLFITHDQQEALAISDRIAVMDKGKVLQIGVP
                     IEVYKSPLNDFVANFVGTSNCIGKEDYVNFNIEKETKPYIYKRPEEMILLQNTNQSGF
                     VKVKVESKNFLGAILEYTVSNDGKKYQVTELNRLNNSNKLNVGDMAYLGVLQGD"
     gene            43072..44766
                     /locus_tag="PC407_000623"
     CDS             43072..44766
                     /locus_tag="PC407_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2496099.1"
                     /translation="MIKKINRSKFTSKNVIFIVLYSILFWILIGYIIIPIGNTFVQAF
                     QGKGGYSFEVFKEYFSNTNNLRVVSNTIILGLGSVFVCGIMGISLALYMTFVCDKHKK
                     IIHILLLSPMMIPGVIMVIACIQIYGESGIVTKSIEILLPFIKKPYDFSGLKGILFVV
                     AYTQYVYFYLNVYVALKYVDYSTVEAAKGMGASKLRVFFDVIWPVITPAVITSTIITF
                     ASGISSFSAPNLIGGGFKVLSTQIVRSKANNHMDIASVQVITLLLMGILFMILLQYYG
                     KKYGVVPSLKSQSYKVHNRKKDLFTFVCKVIIEIQIIMIMLPIVTIVYLSFVSTHSIM
                     TTIFPRDFTLENYQMIFQKSRVLQPILNSFKMSLMAVAVGLIITVPVSYLVIKKDTAY
                     NRLAKFIIMLPWSMPASAIAVNLINTFNQKSIFAFNESLIGGFYIVPIAYTIYALPLL
                     FSSNEVAMKSINLGLEESSRSLGAGRIKTFYNVIVPNMMPGIISGAILVFIRTIGEYT
                     MSALLYGVYNRPISISIVTNMQEFKIGVSMAYGVLVIGICYIALGIIFKLDRKRFT"
     gene            45317..46195
                     /locus_tag="PC407_000624"
     CDS             45317..46195
                     /locus_tag="PC407_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDF2496100.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQGANEKNAPV
                     ILAIHPNELSFLGDSFVVSCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            46231..48126
                     /locus_tag="PC407_000625"
     CDS             46231..48126
                     /locus_tag="PC407_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2496101.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSETKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFPPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVAKPTIRSENS
                     EVELLGA"
     gene            48394..50301
                     /locus_tag="PC407_000626"
     CDS             48394..50301
                     /locus_tag="PC407_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2496102.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDILSESEKVSSIMIENMSQFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            50291..50740
                     /locus_tag="PC407_000627"
     CDS             50291..50740
                     /locus_tag="PC407_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2496103.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNISERLLNVHTKKEILDIFYEV"
     gene            50840..51727
                     /locus_tag="PC407_000628"
     CDS             50840..51727
                     /locus_tag="PC407_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2496104.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGGASK"
BASE COUNT        21435 a         5310 c         8134 g        17046 t
ORIGIN      
        1 atttataata tgtaatattt ttgttttgaa atttagaaat cagtgttact ggttaaagtt
       61 agtttagtat taagtatatt cgaagtagtt ttttagctat tttcatatct ttatattact
      121 taattatata tagcctaact aacttaatat atcagtgaaa tattgactaa aatgcctaaa
      181 tatgttatca tacaagttag tcaaataatt tggcaacatt aaatatttag taaatttcta
      241 acatttatgg aataaatttc taacaaagaa ggtgagattt atatggctgg taaaaaaata
      301 ttagcaacaa acaaaagagc aagacatgaa tattttatag aagaaactta tgaatgtgga
      361 attgaattaa aaggaacaga agtaaaatca ataagacaag gcaaagttaa cctaagtgat
      421 ggatttgcat ctgtagataa tagtgaagta tttttaaaac aagttcatat aagtccatat
      481 gagcaaggaa atatattcaa tgttgattct ttaagagtta gaaaactatt acttcataag
      541 catgaaataa gaaaacttat tggagcaact actataaagg ggtattcact tataccactt
      601 agtttgtatc taaaaaacgg taaagttaaa gtagagattg ccttagcaaa aggtaaaaaa
      661 ctatatgata aacgtcaaga tatggctaag aaagatgcac aaagacgtat agaaagagaa
      721 atgacaggta agtattaaac tagataaaac tgtatttttt agaataaaat tctttattat
      781 ctagaacata acattgattt ttattcttaa aagtattata ttatatatac attaaaaatt
      841 taataaaaat ggtgataaag tttgcgaaaa gtctactgga ctataaacta ctcaaggggg
      901 cgtaaaggtt tcgacgtggg tttggaactt gaggctgcat gtcgtgttac tctgggtcac
      961 gtaaaaactg gggaacttta aaataaacgc agacgataat ttcgcgatag cagcctagtt
     1021 gctgctcgtc cctcttagtc ctcctgccga ctaagactgg acgtcattat gcagggaact
     1081 actttagggg tgtctcgacc tgaagtagat taattgggac tggctaagca tagagcctgc
     1141 cacttggcgc tatgtgaggt gagatattag tgaagttggc taagcatgta gatgcagcaa
     1201 ggaaaagatt tgcggacagg ggttcgactc ccctcgtctc caccaatttt taaaaaagaa
     1261 tgatgaaata cttggaaata ctagtcttta ttgactagtg ttttttgttt tggagatgaa
     1321 atccaccaac cgtgccccaa ccacagaaaa agagattttt ttggggtata ttggggtgca
     1381 tttgattaat ccatggaaca tataaagaat acttttagag atatagtgaa tttgatgata
     1441 atacaataaa tattttatac tgatgaattg tttttatatt atgcttttta tttcaatcta
     1501 ggaaatatat aacttgttta aacttatgat taaaaggata aagaagttta aagcatataa
     1561 gtgctactta atctattaga gtaattacta gttataatga tatacagaat cctataagat
     1621 tatgtataag tatagaacaa atttattatc tatgtgggat gctaatttaa attttttaat
     1681 tgtatttaaa aaaataagat gtaaaaaagg aattgaaaaa tgttaagata ataaacacat
     1741 agaattatta agtttaatac taacaagact attgtatata gacaatatat tatatataaa
     1801 atttaaataa tatatcataa aagtacctat actaataagt acttttatga tataagttaa
     1861 ttctattttt ataatataaa ttaaaagtat ttaaagcgtt gttaaaggct atagtctatt
     1921 attcatcata ttcgatttcg agctgtacag gtagattgaa agctctatta aaaagcacat
     1981 tgaaatcttt ccagtctttt gatgcttgca ttaaggttat aacctttaat aaatgatttt
     2041 ttaaatgatc attacctata tcttctgtta aaaattgatg atgtttatgt tttctttgcc
     2101 caggttttac tgtggggtta actctttgaa gttcttcttt aactccagga ggtaataaat
     2161 cataaacata tttatttgtg tatgttccaa ctattccagg tctcttagaa cttggtctag
     2221 ggtatgtcca gttttttaat ctgaacattt gtctataaaa ttcatcagga aatctttttg
     2281 tccaaggtaa aaactcttta gaaatataca tagacaatat tttttgcagc tcatcacgat
     2341 ctctatcata ttgatatcct gtagcttcat caactaatgc tacaatccct attttggata
     2401 aacttcttac taaaatttcg cttgctatag ctagtccttc ttgctgaaca gctaatttat
     2461 tatcaagcct tgcttgtaaa tatacatcac atataagagg tattatttca gctggataac
     2521 cttccacaat tcttccgttt ttagtcttat attttattgt atttataagc atatcttcta
     2581 attcatctga aatataatca gatagattct ttgcatcaat aaaacttggt agattaggct
     2641 tagaaggtac acgaatttca tctttttttc taccacgctt tgttcttcca aaagctttga
     2701 atactgtact tttatttatg attcttgaac cattttctag tacagcacaa tttaaatctt
     2761 tttcacctat ttttataata ccaaaatgtg ttgctctcaa tatacttttt tcttttgatt
     2821 ccaatttaac accatccttt attttatttt aataagtaag atattactta gtatctcttc
     2881 attagataaa ttagtattta taagactaat aatatcaatt ttaaaatatg tatatagata
     2941 attgagtttt tttatcatct tgtacgcagt atttttatta acaacgatat tttttgataa
     3001 ttttctatag ctaatatttt catcaaatat aaataaatta atagcaagta gccattttct
     3061 gtagtctagt ttagttttat gcataatggt attagttgta attgaaaact tactattgca
     3121 aatattgcaa tgaaataaat cattataagt actaatttta tcagaataac aataggggca
     3181 tattaatgag ttttttaatt taagattata taaaaaaatc ttataagaga catctgttgg
     3241 aaagttttta taaatatcaa agtcttttgt cataatttaa ttatatcatg ttgtagaaat
     3301 taatttactg tatttaagtg ggtcaaattt aatatatatg tttttgatag tgtaaaaatt
     3361 aaaatataat tttaggcatg tttgtattga ataatgagca ctttcaaatt ttattagtgt
     3421 aagaggtttc aaattataaa gtaatgaatg tatattacta tatgatttat aattagaata
     3481 aaatttttat aatagaaatt taatatatca aaatatcagt tgaggatatt gttcaagtgg
     3541 tatatcttcc ctgagttaaa aaataaatga tgaaatattt ggaaatgcta gtctttattt
     3601 gtttatgata ttatgtaaca tagaatttta atatctactt tatgatataa tatttttata
     3661 aacataagat ggtttaagtt attaattaat aaaaatatat atattatata agtggatagg
     3721 agttgaggtt tgtaatggat gaaagaaaac tagaattttc tattttttgc attgaagctt
     3781 tagcagaaaa gttgaatata gattcagaag gagtttataa acttcttaaa catgatactg
     3841 atattttaga taactatata ataccttgct atgaagcact acattctcaa agtaaaagtt
     3901 atatagtgga agatttaata caagtgcttg aagaaaaggg cgtgatgtaa taattgtata
     3961 tcatgattca tactgtgtgg caaaaacgcc taatattatc ttcttaagag attctttaga
     4021 ttttggaaaa ggattctatg taaccagtat aaaaacacag gcaattaact ggatagaacg
     4081 ttttaagaaa aggggtaaaa aagcatattt aaatagctat tctctagata ttgataaatt
     4141 aaaagaaaac tgcaaggtaa aaatcttcaa tagttataat ttagaatggc ttgactttat
     4201 attagaatgt agaaatatga gtgatgtata tatgaaatat gatgttataa taggtggaat
     4261 tgctgatgat aagatttaca atacaataga actgtatcaa gataatctta ttgaaaaaga
     4321 tgaagcttta aaaagattaa aatactataa gccaaatgaa caaatatgta taactaatca
     4381 aaatgtaata gataaatatt taaagtttag atgtgaagag gaggtatagt gcttatggaa
     4441 gcaaataaaa cattattaca gatactatat aaaaaaatta tattagaatt ttctaatcag
     4501 acaggcaaga gtttggaaga aagtatggat tatttatata cttctgaaac atataaactt
     4561 ataagtgagg gtgtatcaga tatgcactgt aagggtcata tatacttggc tcaagaatta
     4621 atgctagaaa atggattaat gtatcataaa ggctatccaa gataaatagt taaaactaga
     4681 tacgaattaa ggatacaaga taatataaaa ttaatttgta tccttatttg ggttttgtaa
     4741 ttaactatat gaaatcatag tatttttatg tatttgattt ttacaatgaa aatattttat
     4801 ccaacttttc tacagcttca accttcttat cttccataac ttgagtatat atatttaaag
     4861 ttattgatat atcataatga cccattaaaa cttgtacagt tttaggagga acatcattct
     4921 caaaaagtct agttgcataa gtttttctaa gaccatgaaa ctttataggt tctatatcta
     4981 agctgcttaa tatagatttt aaattcctta aaggtctttt atcatctata gggacaccta
     5041 attcattgca aaatacataa ttatcatttc tatataattc tccagccttt aatatatcca
     5101 cattttgatt tactttatgc tctttcaatt tatttaaaac atcttttgga ataggtacag
     5161 tcctgtaaga atttgatgtc tttggttctt gctctaatac tttaagctct ctatttccat
     5221 ttttatctat aaaatatgtt ctttgaaggg ttcttttaac tgttaaatta gaatttttaa
     5281 aatcaatatc aaaccattta agtcctaata attctcctaa tctaagtcca gtaccaagag
     5341 caaccaaaaa taatacttct aatttatgcc cctctatagc ttttataaaa ttcttttgtt
     5401 gttcaagagt caatatttca agcctatttt cctttttctc aactggtata tttaccatag
     5461 tacaataatt acgttgtata agaccttgtt tttcagcttc tgataagcag gttttcagtt
     5521 tagtgtttat ttgccttata gtaggtatac ttttattgga atcaagcaat ctattgtaat
     5581 atttttgcat gtgagatact cttaagtcct ttaacttaac attgcctaaa tcagtatctt
     5641 taatataatt acgatagatg ctttcatatc tttcaaatga ctttggtttt aaatctttta
     5701 ttctatattc aaacaaccaa ttgtaaaacc aatgctctaa agttatttta tcatctgaaa
     5761 tatcagaact taaataatat tgttttttat aagcatcaag tttatcttta acttcctttt
     5821 gagttttacc ataaaattct tttcttattg gttttccatc ttcattataa ccaatcatta
     5881 tacgagaacg ccaaccatct ttatattttc cgatagagcc ttctccatta gctcttttag
     5941 ttgccatttt taaacaactc cttatatgat ttatagttat atccatcgaa gaatgtaata
     6001 catttctcta ataagaattt tataacttca tcagcttcta gtgtttcata gaagtttagc
     6061 attaatgtgt tgtattcttg tttatgtttg gctggtattg atagaactcc tatatcatta
     6121 cttataagta ttaggtttga ggtaagtcga gcagttcttt tgttaccatc atagaagaat
     6181 tgacaatagg acaaccataa atttattatt atagcctttt ctaaggggtt agttatttta
     6241 tttatgatac ttatgtcact tataaataaa ttatttaata gagtggcttc tatgcactca
     6301 tattgagtag taccagctat acctatgtta ccgtttctaa aatctccaac aattagagct
     6361 tcatcttttg caactatttt atgtatagag caaattgtat ctttatttaa agataagttt
     6421 tctttgttac ttaattttat tatataatcc catgatgatt ttatatttag tatttgattt
     6481 tggtcactta atttatgtcc acctactgtt ataccatcaa gtaatgtttg tacttctggg
     6541 aatgtgaagg ggttattttc taggttaccc atatcatata caaattcact tatcattctt
     6601 ctaatgtatg ttaaagtata ctttttatct ccatgaggta gattcgtagg gaatttatgt
     6661 gtgtattttg gtatatatga tttattcatg attttagctc ctttcatatc taatttttat
     6721 ttattaacta tgaatgtata aaagttaata ggtaaaccca tattttgaaa atgtgcaata
     6781 ctaatattct tcatcctcat cttcaataag accattagag ttttcttgta taaaattttc
     6841 aacaagacca tttatcaccc aatttagatt tttggagaag tctttgaagt cagagaagct
     6901 taagctaatt ggttttattg tatctgcatt atctgtaatt tcaaactttt ttttatagtc
     6961 tgtttttata tcataaccat aattttctaa tatgtgaact actaaatcca actctttatc
     7021 aaatactttt aaattaatat tattatttgt taaagtatct aaatcatttt cttcaacaat
     7081 gagtttattt atggcaattg ataaagcagt agatattttt cttatcatat ctagactagg
     7141 tattctttga ttttgctcat atttagctat agcatgaact gaacaaccaa tcttgttggc
     7201 tagctcattc cttgacatat ttatcgattt cctatattgt ttaatctttt ctccgattgt
     7261 attatttatc ataattccac cttaaatata tcatcaaaca atgattgtat ttttacacat
     7321 ataaaggagt tattttctag attatctata tcatatacta acttactcat aattctttta
     7381 atgtatgtta gagtatattt tttatctcca taaaataaat tagtagggta tttatatgtg
     7441 tattttagca tatatgcttt attaataatt gtaactccat ttgtagtttt ttatcattgc
     7501 tttagatagt attgatatta ttttatattt tagttagtag tttttttatt tctttaagtg
     7561 tattttcaag ctttttattt ttttccttta attcattatt ttcttgtaat aatttaaagt
     7621 aatcattatt tttaatagtc atattttctt tttgagatat taactttttt agtcgaatgt
     7681 tactcccatt atctgttgcc tcactaaagt aatcatagat tgcttctata ttttcttctg
     7741 atagaccaat atctaataat tcatcaattg attgttttat aagaacatca tttttaataa
     7801 tttcttttat atcatcagga tgtaatgaaa aatctaattc agtattagat acacaatctg
     7861 aaagtatcca attataaact tgattttcat ttagttctaa tttttcacca agtttataat
     7921 attggtcttt atcagaccaa gcacctagca ataaacttga ggtaatccta atatcatcat
     7981 cttccataag atttgaaata gcatttaaaa attcatcaaa agaatagtat tcttctggga
     8041 ataaagactg taaaaaatat aaaacatctt cattagtcat tacttcattt tcaatatcaa
     8101 aataaaagat agtcttttca atttttaaag ctttagcgag ttttgataag gtatctatac
     8161 taggtattct tttatcattt tcataattta ttagagcatt tcttgagatg ccactaattt
     8221 cagcaagttg aatctgtgtt agttcttttt gttttcttat cttttttaag ttttttccaa
     8281 tttgcaatat tatcacctcg tataaagtat atcccaaaag tacacaaatg agaacagaaa
     8341 aataatatat tcttattgac agcacacata tgtcgacatt ataattaaaa tgtaaagttt
     8401 acgaatgtaa actagaaaaa aggaggaaaa agtatgggaa tcaatttaaa aatatttagg
     8461 attaaaaaag gtttaaagca acaagaactg gcagatagag ttggtatatc aagatattat
     8521 ttgtcaaact tggaaacagg taaggctaat aatccaagca atgatttgat gattaaacta
     8581 tctagagctt tagatacaac agtagaagaa ttatttttta gtaaggagga gtaaaatatg
     8641 aaagagaata ataatttaga agttttaaag acattacaag ctactttaaa cattattgta
     8701 aagaatgaag aagaagcaca agaaaaaaag caaggatata aaattattga aaatgcgaaa
     8761 agaaataaag ctgaacttat aacatgtaaa gaagcatcta agctttatcc tataggagaa
     8821 tcaaaattta ggcagttatg tcactctaaa aataaaggtt tcccttgtat atggataaat
     8881 aatcgagtat acataataaa aaacaaattg gatgattggt ttattgaaaa tgcaaatggt
     8941 atcaagttat agtcttaaag taggtgtttg caattgagca ataaagggtg gataaagctt
     9001 tacagggagt tgttggataa atcaatttgg aaagatatta aaccagaaag aaaagtaata
     9061 ttaataactc tattacttat ggctagtcat aaagaaaatc atgtttttat agataataaa
     9121 aggataaaaa taaagtctgg acaatttatt tcttcttata gcaaaatagc tgaaagatgt
     9181 ggtaaaggag ttagttatca aatggttaga agttgtttgt tggtttttaa aaatgctggt
     9241 tttctaacat acgaaacaac aaacaaatat atagtcataa ctattgaaaa ctgggagctt
     9301 taccaagaga acatagaaaa tcaacaacct aatcaacaga cgattaacaa tcaatcaaca
     9361 acaatcaaga atataaagaa tgaaaataat attatatata gtgaaattat aagcaaattt
     9421 aactcaactt gtattgaact ttcaaaggtc aagaaactaa cagacacaag aaagaggaga
     9481 ataaattcaa taataagaga atatgataaa gaagctgtat tagaggttat aaatatagtt
     9541 agtaaaacaa gttttctgaa tggaaataat aatacaggat ggaaagcaaa ttttgattgg
     9601 ataatgaatt caagtaattt taccaagata ctagagggca attatcatag caaggaaatt
     9661 gtcataaata aagagacagg aatatataat ccagatgatt cacaattaaa gtggtaagga
     9721 ggttatatta taaataattc aaacaactta attaaaaata cagaagcaga gcaatcaata
     9781 ttaggaagca ttttattaga ctcaacaata gtagaagagt tagagcaaat gttaaatgaa
     9841 gacatgtttt atcatgagga acataaatac atatttaatg ctataaaaga aataaataag
     9901 agaaatgaag taatagatat aataactctt acagaagaat taaagagaaa aggtcatttg
     9961 aataatattg gaggtataag ctatattacc agtttgtcaa caatagtccc aacagtatca
    10021 aatttcacga gttatgtcag tatagttatt gaaaaatata aaaatagaaa tataattgat
    10081 aaattcaata agttcaatct tggagaaata gatgataata ctcttatata tcagttagaa
    10141 aatgatataa ataaagccaa tcagaaatta ataaaaaagg attcaagtat aacagccata
    10201 tcagagagag cctttgaata tataaatgat gaaatgaatg aaggtattaa gactggaata
    10261 aagttcttag atgatactat aggaggttta tatggaggtg aactaactac aatagcagcc
    10321 aaaagtggaa gggggaaaac agcattagct ttacaaatac ttagaaatgt aatatttcaa
    10381 ggaaagaaag tactttttat aagtggagaa atgagtgata tacagattct gtttagaaat
    10441 atagcctcac taactggaat atcagttgta aaaatgaaaa atagaaatct aaatgatgat
    10501 gaaatagtca aatatataca agctttaagc ataataaatc aagaaaataa cctttatata
    10561 aatgactctg taaatacagt atcagggata aagagagaaa taaagagtac taagcctagc
    10621 ttagtcataa ttgattattt gcaaattttg gattatgaag gtaaagaagt aggagaagct
    10681 aaatttgctg acttatcaag gcaaataaaa aagattactt tagattttaa tataccagtt
    10741 attcagcttg ctcaattaaa tgatgaattg aaggatgaaa gacctaaagg ggatagagta
    10801 atgagaagct caaagcagat atatatggat tctaattcag ttatatatat tcatagacct
    10861 acagaaaaag aggctcttaa ttattgcaag acaatgggaa ttgaggatat aagagagttg
    10921 agacgaagag aaaaaggttt tggtgctaat ttaagtgaga ttattgttgc caaaaataga
    10981 gatggtgagt caggatttag accatattgg tatataggaa atttgttttc ttttgaagaa
    11041 tgtaagaaga tacaagagat ataaaaaaga agcatgattt attaagtaat agataaatta
    11101 tgtttcaaga agtattgaat ggtaaatata taaaacttaa ttagatgtaa tagagattta
    11161 aaatatattg agtttagatt taaatgctta ttagaaaaag gtagttgcca tataaatata
    11221 acaactacct ttataaatta ggtacttaat gtacactaac ctagcaactt aagtatatat
    11281 taagtacctg atgaaagtca aggaggtact aaaatgtatg atgaaattat agaaatatat
    11341 tttgattatt ggataaatga acatatgtat agaagtattt tagaaaaaga tgagtattat
    11401 attgaatgtt ggaaagaggt taaaaaaatt aatgagttat tggaaataaa tttggaagat
    11461 gataagaaat atctgttggt tgaattaaat gatttgttta attcattaat agaatttgaa
    11521 agagaactta gttataaatt aggatttaaa acaggagcaa aatttataat agagttgttg
    11581 aagtaattaa tttgtgtatc tcaatagaat aattaaatag taaatcgaga ttattaataa
    11641 gttgtactag aatattttaa gtgaagtata tattaattat attgtttaaa ataaatatat
    11701 ttaataaaga ggggataagt atgccctctt ttatttttat gcaatcaatg acattgtagg
    11761 atttacgtat tttgataaat agcacaatat attttaataa taaatatatg atacaataat
    11821 tttattaaaa tctaaaataa tcaattgagg aggagaatat gataatacct aagttagagc
    11881 ctaatgatag aaaaagagag tttaataaat actcatcaga gcaaataaac aaagtagttt
    11941 ataattattt atttaaaaat aatatgagta atagaaagat agatgatgag atactagatt
    12001 tatcatcaaa acaaagtaaa ggttatcaat caatgggtat agtgcatcat ttaggaataa
    12061 ataaaaatca taaaggtatt tttacaggat acacaataga taaagcaatt gaagttctta
    12121 aggaagatga gatacatttc aagcagatta ttaatatact tttagaaatt agaaatataa
    12181 atgagtatcc tataatagtt gactcttggg agttattaga tgagaaaata gctttaaaaa
    12241 caattgataa atctacatta aaacataatg ggacaggagt tccaatagaa ataagatatt
    12301 tttttgagat aaataattta ggaaagaatg aaaagaaaaa tgtgaatttt aaatataatg
    12361 ataattatta tagtgcagtt atagagatgg ataaattaga gaatccaaga agtagaataa
    12421 tttggaaatc taagtttaga gatttactaa gaagtatatt ttctgagtat tatgagaaat
    12481 tcttagaaaa taaaatagaa ggaaaaacaa atttacctaa aataagattc ataaaacttg
    12541 atagtaataa ttttaacata gaattcattt ttactgctga aatagaagac gatgtattag
    12601 aggagataag tcaagataat cctttgaaca ttaatactat taatatagaa ttagaaactg
    12661 aaattagaaa agaagggaaa gttaaatatg tttatgggaa acagtatgag cgtaacccca
    12721 gaaatagaat agaagcaata aaatatcatg gcacaaagtg tattgtatgt gggtttgact
    12781 tcgaaaaaac gtatggagat agaggtaagg gatacataga aattcatcat attaaaccat
    12841 taagttctgt tggagaagaa agcaatataa atcccaaaac agatttggtg ccaatatgtt
    12901 caaattgtca tagaatgatt cacaggaaga aagataatgt tttgagtatt gatgatttga
    12961 agcaaattat aaaagatagt aaataatgct tatgtgtggc agagatgtat aaagtatata
    13021 ttctaaacta gttaacaaca aatactaata taaaaaatca aaaaatttat taggaaaagg
    13081 tagttgctaa agtataacag ctacttttat aaattaagta tttgaggtat attagtctaa
    13141 ccgattaggt atatcttaag ttattgatga aaattaatta agactaacat gtatgatgaa
    13201 tccatagaaa tgtattttta ttgttaaata aatgaacata catagaaata ttttagaaaa
    13261 ttacaagtac tatattgaaa gtttgaagga gaaccttgat ttaaggtaag tgtaaagttt
    13321 ttagaaactt cactaaaata atctaattaa gttatatatt tgttactttt aggaaaataa
    13381 tggtaaaaat taaaggtttt tataaatgaa tgtctaattt tataaataaa atggagtatt
    13441 ttaactaaat atttgaggag ataaaatgat attaaataaa atcgaaataa ttggagatat
    13501 ggctcacttt aaaattccta tgcatagcaa gatacaaaga acatatgaaa ttccaccaat
    13561 atcaacagtt gtcggaattt taaaaaatat atatggtgaa gaaatagatg attttatatt
    13621 aggatatgtt atatatcatg atggtacatt taaagattta cagactatat ataaagaaat
    13681 caatccaaat gtaaaaacat tgacagatag cgatagattt caaactgatg tatgtcccat
    13741 tgaataccta attaatccta aacttatcat atatactaac atagataaaa atatagaatt
    13801 taatgagccg ctatgtttag gtaagactaa ttgtttagcg aaggtattat ctttaagtga
    13861 aattgaagta aatctaatag ataaaaaagc tattggatac aatcaatata cagatattaa
    13921 tattggagat ggaatgataa aaagaataaa tacattgaca gagtataatg aaaaaaaagg
    13981 ttattatgat atatattctt caatggtaag agaaaatgat gagtttgaat atggtaaata
    14041 ttatgatgaa gatgagcaac agaatatttt tttatggaaa tggaaaaaag ggggagtaat
    14101 taaatgcatt tagtaagaag acatttgata ctcacagtgg ataatgaagt tgtattagat
    14161 tacaattacc agtatgagct aatgaaaagg atatatgaag ctatagaaat taatgataaa
    14221 agaaaagcac taagtcttca caatgaaggc tataaggtgg ataagaaagt atttaaatta
    14281 tttaattaca caattatgtt tgaaaatgct aagtatttaa aagagggaat acatttaaat
    14341 ccccaaacaa aaatcaaatt aattttaagt ggatatgatg atatattgaa taatattata
    14401 aaaggtttta ttaaatgtaa agtatttaaa ttgaataata tagagtttaa agtttcagat
    14461 atagaagaag attcaaagaa aaattttaat aatataacac tttataaagt aaggtcacct
    14521 attgtagcaa gtttatatga tttaaaaagt aggaaacaag tatacttaaa tccaatgcaa
    14581 gaagagtttt ataaagcatt acatgataat ttaggaaata agtataagct tatacataat
    14641 aaagaatata ctggtgaact atactttgat attgaggatg tattagcagt aaaaaagaaa
    14701 tatataacta atataaaagg aaaaggattt ataattggat atacagattt tgaaatattt
    14761 gtacaagcta ataaagatat gcaagaagta atttattatt gtggattagg tgaaaaaaat
    14821 agtataggaa tggggttact aacttatata acttctagga gggcttaata tggatagaag
    14881 ttacaatgta tttgttgata atggtctata tgtgttggct tattatctga acaaagatat
    14941 taatgatata acttatcaag atatagagaa tagtatagat ttaatgagtg ataaaattga
    15001 agaatttgta agttgcgaaa aatactctaa tttaaaaagt atgtgcttta gcaattctac
    15061 gctaactcaa ccaaaaggaa aagttacttt aaatgagaag ttaaaaggtt ttgttaaaaa
    15121 tcaaggagat gaatattgta gtttatgtgg tcaatataag gccaaagtca aaattgaaga
    15181 taaagaatac aatataggga gaagttatat gcctaatttg gttgcaaata cattttataa
    15241 tttctccaat aatttgcaag gtttgaatgt atgtccatat tgtttagtat tgactatgta
    15301 ttcaattctg aattgtagag taagtaggta tgctttttta tacaattcta catcaagcga
    15361 atttatggaa gattatactt tccatattca aaaagaaaat ttaactgatg tagagttagg
    15421 agcaaagaaa gaaaaagaaa agtattcaat tgtaaaatta ttagaaagtc tagtgtgtaa
    15481 atataactta tttaatggtg atatagagca atatatgttt aataactctg gtcaatctca
    15541 ggatattcag gtaaatagaa taaaaaataa atatgtgaat ttattaataa aattacagga
    15601 aaaagcacta ctatcaaact ttgaaaagtt acatttggat aggtatatct tgaatggtac
    15661 tttagaaagc aattatctca gagaagtata tagggtaaaa aaggaagaaa aaatggatga
    15721 gaaagaacaa aagaattttg atgaacttgt tattttttta aacagggagg taaatgtatt
    15781 gaaaaaagtc aaaatagata taataaaaaa attaacggat aaattaaaaa ctagtgactt
    15841 agatatagac aaaatatata aggagcttaa aatgttagga aaattttatg catatgagga
    15901 ctggttagtt aacttaaatg aaatgtatta tgaaaaaact ggagaaaaac tatttgaagc
    15961 agaggattat attgagttgg atgatataag aagctttaga cagattaaaa atttaatatt
    16021 agtaagttta atttaataat attatggagg gttaaaatgg aaaataaaaa ttcaaataga
    16081 atgttaaata taggctatgc aatagaagtt ttatgtgggc gattaaatgg aagtagtagt
    16141 gaagtagaaa agaaaaataa ttcatataag aattctactt atactaaaaa aatagggagc
    16201 agaagtagaa tctcaagtca atgtcaaaaa tacaatcaaa agctatttat ggaacaagtt
    16261 tgtggttatg aacaaaatgt taaaactaaa gatgggaaac aagtaaaatt ggttccaaac
    16321 ccatttaaat atgcgacaga tgatatattt gggtttatga tggcggaaaa attatctata
    16381 gaagaagatg tatacaaaga attatcagat gaagaaaaag aaatatataa aaaaaataaa
    16441 aagaaatatg aagtaaatat aactaaaaaa agaaagtcaa gatggcaaat gaactcttta
    16501 ataaatgtat caaatagaaa agttgaatgg gagtggaatg tttgtggaac tacaggagat
    16561 agtatgccat atcaagttga ggtatattca ggggtacata caaatatagc aaatcttaat
    16621 ataggaaaga taggagaata tgatatatct gatatagcaa gtgagcatag agattataca
    16681 actttagaag ctaaatcaat tggagttgaa aagttagata aagaagaaaa gtttaaaaga
    16741 atagaagctc tcctaaaatc actagaatac ttaagtgtag aaggtaataa gacaaatcat
    16801 ctaactgata caaaacccaa atttgttata ttgggagagt attcatgggg aaataatgta
    16861 tttcagggta ttatcaaaga aaatggctta gatgtagaga tgttaaaaga atctatagaa
    16921 caaaatgaag aatttagaaa aagcgatata tggattggaa taaataaatt ttatgatgat
    16981 acatttaatg agatagaagt taataatgaa aaggaacaaa taaagaagta tttagaagaa
    17041 gagtttgaag agtatgattt tatgaaaata tcaaatgtac atcaagcttt tgaaagttac
    17101 ttaaaatatt taaaggagac tttataaagg atatatcatg aataatttat atgaatataa
    17161 agctaaatca gatgggacaa gtatagataa gcataatgaa gatatgaatt acataatgaa
    17221 tcaattagtt agcatatata atatatctaa tgatatgaaa aatgatttat atgaggcaat
    17281 tgtatgtcat gatgtaggga aagtagtaaa aagctttcaa gataatattg aaagtagcac
    17341 aagaagagtt agacatgagt tattatcagc aagtattaag tatttaaata gaggtcagaa
    17401 gttagctata ttaacccacc ataaagatat aaagtttcta gagggtcaaa taacaaattc
    17461 tatttatgaa attgaattaa aggaattaaa ggaactatta gatattgaag tagaagatat
    17521 aagaccattt ataaaggaaa tatcaagagc aagaaatgat gatactagaa aacttgaaaa
    17581 tatattatta aaaggttact tgcaatattg tgaccatata gcttctgcgg gaattaaaag
    17641 tatagataaa ggatttaatt ctattaaaga ttttagtata ccaaagggtg taagttataa
    17701 ttcaatacaa gaaaaagttt taaatatgga taaaaatgaa gatataatga taatggctat
    17761 gacaggtctt ggaaaaacag caacatcatt gtattggagt aacctagttc aaaatgaata
    17821 taaaagtaag agagtgtatt atatacttcc ttatacagca agcataaatt ctttatatga
    17881 aaatttatat aatagaaata taagcgtaag tatgttacac agtaaagctc catatttttt
    17941 gaataaaatt agtgatgaag aagatataaa acaactttac cagatataca aaaagagtgt
    18001 aaaacaaatt aatgtatgta ctatatttca attagtaaag tcagtatttt cttgtaagag
    18061 atttgaaatg ctactatcac aattaaaaga tagtatattt atagttgatg aaatacattg
    18121 ttttgataca agagtattgt gttatacatt ggaattttta aaatatttaa aagaaaactt
    18181 aaatgtaaaa atatgtataa tgagtgcaag tataccaact tgcttaaaaa agttaataca
    18241 ggaaagattg caaataagta caattattga accagatagt aaagatttaa tatttagaca
    18301 tagattgaat agattaagtg aaggaatact tgatagcata cataaaatag aaaatgattt
    18361 aatgcaagga aaaagagtat taatatgtgt taattcagta gatttatctc aagagttata
    18421 tggaaggctt caaaaatata atcctaaatt aatacatggt cgatttaata ctagagatag
    18481 agagcaggca gaaaaaggga tagaagaaag cttcttattg ataggtactc aaagtataga
    18541 ggtaagttta gatataagtt atgatgttct ttatacagag ttagctcctt tagattcatt
    18601 gttacaaagg tttggtagag taaatagaag gggagataat gctgagttga gcaatattta
    18661 tatatatgat tatgatagaa agcttaatat atatgatgaa gatataataa ataggactga
    18721 tatagtaata tcagatataa tacataatga taattctata gttgatgaga gtaaagttcc
    18781 atcttattta gataatgtct atttagatat agatatggat aaatataata aacataaagt
    18841 tgctttagag attataatga aaaatatgag gttaggggta tggaataaat ctgctaataa
    18901 tgatatgtta aataatgata ctattagtgt tgtgcctgaa tgtcttttag aagagtacaa
    18961 gttgtatatt gataaaaaaa tgtatttaga agcgagttca ttatttgtaa atgtatctgt
    19021 aaaaagatac aattataata aaaagatgtt ttatgaatgt ggagatatag ttgttacaag
    19081 ttatagttat aatgataaac taggtctaat atttgattgt taagagaatt atagtataac
    19141 taattaaaat attttattga gaatttgtta taatttatat cgactacaaa ttttatatgt
    19201 tacaatagaa taattttgca gtgagcgaaa aatatgtgta aatggctgtg agttagtgat
    19261 ggcaaggttt ataactgaat ttttgttttt agctaaaaac actactggtg cctcactgca
    19321 aattaactat tttaataacc ctacaagtgt tgtaattact atagttttat ttggggttta
    19381 aattacacta agttagttat aaatattccg gaaagtactt ttgctatttg attcattcta
    19441 cccgtttaaa ttacactaag ttagttataa atttcactaa ttttagttac aactattaca
    19501 agtccagccg tttaaattac actaagttag ttataaatta tgtgtaatgt attttaatgt
    19561 tttctagcaa caatgtttaa attacactaa attagttata aattttataa attctatgaa
    19621 taattttaaa gagaggtttc gtttaaatta cactaagtta gttataaatt gtacatttga
    19681 catagaagct tcaaaatcca tcccagttta aattacacta agttagttat aaatgtaaca
    19741 gcacgaatga tggcacagac ctatctgcaa tgtttaaatt acactaagtt agttataaat
    19801 agaagtacac ttacaagtaa gataaaagac ccaggtacag tttaaattac actaagttag
    19861 ttataaattc tgtagctata tgtggaagta attttagtag agaggtttaa attacactaa
    19921 gttagttata aattattgaa ggggtgagag ttgtgactag gcactacaag tttagattac
    19981 actaaattag ttgtaaattt acttattcat taagaaactt aactgagagt ttgatttata
    20041 ttattgaatt agcaagagtt ttagaaagta gaaaattata ccccaataat accccaacca
    20101 caaaaataaa taataaaaaa tatccaaaaa aattagtata acatcataac taaatagcta
    20161 tatacctaca cttacgaaaa ccaacaaaac aatcaaataa ccacttgtag actcccctcg
    20221 tctccaccaa ttttgaaaat gaaaataata gaataaaagg aattacttat gtttggacta
    20281 agtaattcct tttattttgc ttaaaagcac acaattagca cacaactaga aggttatata
    20341 aataaaaagg ctgtcgcact agaaaatagt gctacgctct tttttgtata aaaaaataaa
    20401 tcccaagttc aaaagaacct gggattcttg tataataact gtatgctaaa acaaaatact
    20461 acacaaattg attatactac ttatggtgat aattatcaat ttaagctacc actaaatatt
    20521 gattacatga ttccaaaaga tgattctgta aggttattaa gttaaattat tgaggagatg
    20581 aatttagaaa aattatataa gacttattct cgaattagat gaaatagagt aatccataga
    20641 caatttttga aagttataat atatacagat atgaactgta tttattcttc gagagaaata
    20701 gaaagaacat gcaaaagaga tattaatttt atgtaccttc taggcgaaac ctccactcca
    20761 gaccattcaa caattgcaaa atttcgcagt atacattttt aactaatagg caaaagcttg
    20821 attgcacagt ttacaaactt tcttggagat aataatgaaa tttcaaaaga cacaatattt
    20881 attgatggta caaaaataga gtcatctgct aatagatata cttttgtttg gaaaaaagct
    20941 gtaaataaaa atatggctaa aatggtgaat aatatatctg acttcatatt gaaatgtgag
    21001 attgatgttg gcattaaaat agtttatgaa gataaaatta aaatctatca tttaaagaaa
    21061 ctaaaaaaaa tatcaaaaga agcaaatgta gcatttattc atggaaaagg aaatagaaaa
    21121 tctactaaaa accttcatat aatgggtgaa cgaaatagtt gttctaaaac agataacgat
    21181 gaaactttta tgagaatgaa agaagatcat atgaaaaatg ggcaactgaa accagcatat
    21241 aatatacaat ttggtgtaga ttaagaatat atagtttggc taaattcagg aaatcaaact
    21301 gctgatacta caacattaat accattttta aaatctatag aggaatatac taagtttaaa
    21361 tatcctaaag taatagctga tggtggatat gaaagtgaat aaaactatgc atttttagag
    21421 cataataatc agttatcttt cataaaacct gcaaattatg aaattattaa aactagaaaa
    21481 tataaaactt atattggatg tattgacaat atggattatg aagaagaaaa taattattat
    21541 atttgtacaa atgagaaaaa actatttgct aataaaataa taaataggaa atcaaaaaca
    21601 ggatataaaa gtgaaataac atgttacatt tgtgaagagt gtagtaattg tcaatataaa
    21661 agcaattgta ttaagagaag taatagtaag gttcctctta aaaataggac taaaaactta
    21721 caagtatcta agtcgtttca tgaaaaaaga aaagaaaact taaagagaat tatgagcctc
    21781 gagggggatg aacttagagt gaatagaagt atacaagcag aaggagcttt cgctcaagta
    21841 aaataaaata tgaaacttac aagattttta agccaaggta aagaaaatgt tttataagga
    21901 tatattattg tggcgatagc ttataacgtt tctagactac acaataaaat agtaaaaaat
    21961 aaacgtggaa tatatcttta tcatttaaaa aaatattcta aaattattat ttataatcaa
    22021 atattatctg aaaaaatggt atttttatat ctttatttgt catgcataat tttacttaga
    22081 acagaaactt tatataaaac aattgaaaat atacctatta agataattag aaaagagctc
    22141 tcacagaaca aatttaattt gctcatgcaa cagccccata ttgatataca tttaggttgt
    22201 ataattatac atttcaatat tacatgttca ttaggataat agttagtaaa tgatatgatt
    22261 gagagaatag atataataaa ctaatctagt cctgtattgc aatgtttatt aaagaatata
    22321 tttttaaaga tccaagtact gcatctcttc cagaattgag ttggttaata gttttttgat
    22381 gctatgcaat ttataataaa aagtataatg atttagttga tgaatcatca gaatttatac
    22441 cctcaaagat taatcagatt gcaataaaaa taatgatata ttctatagtt ataatatcta
    22501 ttttctttgc aacccctcat acaatgaata taaatatatc aaacttggat atgggtataa
    22561 ttctcatttc agttttattt atacagtttt tatctaaatt aattttgtat gtatattttg
    22621 ataaaaggga gatatgtaac taagtggtaa tattggtgaa aacaaaaatt aaagaatata
    22681 gaaaaaaact ccttatgaca caaaatgaac ttgctaaatc agttagggta aggtgtgaga
    22741 ctattgtaca cttggaaaat ataatccttc tttaaagtta gtgatagata ttgctaaagt
    22801 attcaatact acagtagaaa atttgtctga atttattgaa gaaggatagt ggatagatta
    22861 taaatagtta taagatggtt aatgaaatat agttacttaa ttattaagtg tgttagaaat
    22921 atttatatgt aacacaatgt gtagttaaga ttaccttatc tcatctaaga ctttaatttt
    22981 tcttcccata aagagggtat atcaattttc tagtgtggta gcccattttt tattgattta
    23041 aatttataat atatattaat agatgatact taaaaatttt ataaaatatc taagttgtca
    23101 taaataaaac gatatatcta accgaatatg gtaaaatttg ttataatata gataagttag
    23161 agtaaaaata attttaatgg gggagaaaat gcagtcagta atatgtaaaa tttttgataa
    23221 taaattacta attagtaatg atttaagtga gcaggatgat gtattatatg aggtaatgga
    23281 taaatttata gatgatattg aatatatttc aaaattagga gatgagcaaa ttaaaagtat
    23341 gcttatggag gtaaataaat ttgaagaaga ttcaactgaa tttgaactat atatgaaaaa
    23401 cttatgctat ttaaagcaat catattttat agatactaat gataaaagtg aaataaattc
    23461 tatagatata gatatattaa aaaatgttaa aatagaggac ttagttgata taactaatat
    23521 taaattagaa cgaaatttaa atataaatat agaacatata tctaaagctg taaataaaat
    23581 atatgaagaa aatgaaaagt ggaaagagta taatatacaa aatactatgt ctaaatatat
    23641 aggtatacct gaatcagaag ttatatttat atcaaatttg agtcagtatg tttcattttt
    23701 atctgaacta gataacgcag gcgactatgt atctagaggg caaaaagatt gtatgtatga
    23761 attattacca tcattacata gaaaatataa aaaagactat ggtatacatt cagatgaata
    23821 cgaggaagca ttcaaacaaa aaataattta ttatgataaa gaaataaaaa atagatcgag
    23881 tgaagaatta agagctgaag ggcaacattt tggactacca acagaatatt tagatttcac
    23941 agaagctcat ttaatatcat tattatttgc aatagaagac tataaatata caaaacagca
    24001 ttcaattgtg tactttatag actctatttc ttataatact gacactataa aaagatctga
    24061 gaagttagta aattattcag atacatctgc aatagataat tgtagtaaat tttcatcaag
    24121 atcatttttt ataaaggtag ggaattcaaa tgaaagaata cattttcaaa aaggatgttt
    24181 cctaaaagta tcatcagaag ataaaggcga atttaaggaa aaattaaaaa agtattgtaa
    24241 agtagtagtt attaataaag atgctaaaaa agaaatatta aaagaattat ttaatttagg
    24301 tattacattt gaaaatattt atccagataa agataatgta gttaaatcta taaaattcta
    24361 ttatgatgag atagtagggg gaaatttata atgagagaac aatttaaaaa ttctataaaa
    24421 aatattaaat caaacagacc tcttgtatca gtccaagata ttgtgaattt tattgataaa
    24481 ggagaaaagt acttgaaatt atcaatagat gaaatagatg aaatggaaat tttacaagga
    24541 atagttatga gtcctgatta tcaacgtaca tataaatcat caaacaagga agaaagttct
    24601 ataattgaat ctcttttaat tggaatacca atacctgaaa tatttcttgt aaaaataggt
    24661 gtaaatgata tgcaaataag acatgtaatg gatgggcagc atcgtttgaa tgctatatat
    24721 aggtatgtta agaataaata tccacttaaa aatttagata tattaggcaa tgacccacta
    24781 tatgataata aaaggttttc agaattagag aaaaaagata aaattaaaat attaggaagt
    24841 catttatcaa ttctagaatt tgatggtttt gataatccag aaatagaaat agaattattt
    24901 aaaagatata acagaaatac aaaaccatta gagatgcaag aaatcgagat ggcaacatat
    24961 ttttcagaaa ctagtaagta tataagtaaa tttataaata ctctaataaa agataaatat
    25021 aaggatgaaa tagataaatc agaatataaa tatgctaatt acaaatcaga tttacttaga
    25081 atatataaca taactaaatc tcgtaatgat aaacaaaaaa atcatcaaga aatatgtata
    25141 atatttagta ttttagaaaa tgggctacaa gaaaatatca gagatggagt tacagcctct
    25201 aagaaatttt tagagtataa gtcaattcag tataaaaata atgaaagtga aaaattagag
    25261 ttattaaaag ataagtttga tgagtttaat aggatgatac tgaaaatatc tgagaagata
    25321 gaatatccat tctcaacaag tttaatatct gatgataagc aaagacaaag taaatacttg
    25381 ataggagtat ctattgtgtt ggcttctatt tgttattatt atgatataga cttggaaaat
    25441 aattatttaa tacaagatat taaaaaaata atatcttgtt cacccatagc agatatagaa
    25501 tataaggcgt cttcaactaa tatgaaaaat atgattctat acttatttat aaagaataaa
    25561 atacaagata ataaatttga atcattaaat ttaaaaagag ataaaattaa agaaataaat
    25621 actttagtaa tgaataatga tggttattta aatgaacaga caaggctatt agaaatatat
    25681 taacaagtta taaaataaat catggtaatg taaaataatt tgtgctttta aggttaataa
    25741 aaatcttctt tatggcaatt atttaaatat aatcaaatgt cgtaaaggag attttttatg
    25801 aacgctttaa gaaaacaatt agccaaagaa attatatcag atatctaaaa aagagcatgt
    25861 caaataaaac cctaggtatt taaatttttt tagagctact taaaataaat tatgctatct
    25921 ctgtatttaa atatttccta aattcaagtg gagacataga atttaatctc ttttgatatc
    25981 taaaattatt atagtaatat atataatcct ctattgtttt gactaattgt tcgtatgtat
    26041 caagttttct taaatagtac atttccactt ttaaaattct ccaaaatcct tccataggag
    26101 cattatcaat acaacgagca actcttacat actttgtatc atgccttgtg ttaaaagttt
    26161 tgatttaaat accttactag tatactggaa acttttatca ttatgaaaaa taggttttgc
    26221 atttggatat agctctattg ctttatcaaa agtttctaac accaagttat tgttatttga
    26281 ctttccaata acatatgaaa ctatacttct atcgtctaaa tctagaattg cgcttaaata
    26341 ggccttcatc ccattggtta atttaaattc agttacacct gttaaccatt tttgagaggt
    26401 tttatctgca ctaaattttc tacttaatac attttccgcg atcacttctg gcgtacattt
    26461 aacataacta aatttctttt ttctgcaaac agaacttaag cctacggctc tcattagtct
    26521 gtacactctc tacaatcttt aacaatttca actctttcat ctaatgctgt ttttctagtc
    26581 tccataaaga tttctccctt cgaggtttct tttatctact tatgaggatt atactcttta
    26641 ccccaagctt ttagtgaata aactgatctc attttatatt tttttgagat tgtgtcataa
    26701 gatccttcac catttagata agattttata gcattcaatt taaattcttc acaatagcat
    26761 aatttacaac tttgttttat taaaccatca tttccaaaag cttcataatt tcgcatccac
    26821 tctctgaatg tacgtttatc caatatttaa ttcttccatt atttttatta ctgtattctc
    26881 accttttaaa tatgattcaa ttgcttcgat ttttttgctc tgaagatatc ttagttttag
    26941 atagtcttac atattttaac tgtctcctaa gatgagtata attcattcat catcagttct
    27001 atagaataca cctagttcct acttactgta ggaactagga ctatatgaaa cccattcata
    27061 gattgtagtt tcttctgttg cattatagcg cagaatagta aattcatgtg aaattttatt
    27121 ttgaatttac tatttatata taaatgaaga gtggtcttat tttcaattaa aatttggaac
    27181 aattttgggt tgacatctac aattaaggat ttaagttttt gttatactaa taaataaaaa
    27241 cagagattca aaatatataa ataatactta aactagtgct gatagatttt ctttaagttc
    27301 tagttttttt attgtcatta tctaatatgt actattttat gtacagtcaa tataatctga
    27361 aattgtaaaa tggtgtataa tatagttata agtaaagaag ggatttaaac atgtatgatt
    27421 acaaaaataa aaaagactta tatatacctt aaggaaatac aaaaaatata caaaatcaat
    27481 gagcataaag aactagtaca cataataaaa aaactaataa atgataataa aataaaacca
    27541 ataaaaacct cagatctaac acctcagcat gagcaattat atacaaagta tagaataata
    27601 aaacaagaaa cagaagaaga taaaaaaata ttagaagaaa taaattacaa aatatgtgat
    27661 aaattaagta tagattatta tagaaaaaac ttagtacact ataaaaataa tagagttgca
    27721 ataatggatt taaatagtta tcttattaaa aaatctgata atctatcaaa gaaaatatcg
    27781 attaacgaaa gatcttatga aatatttaag gatgagaaat tcataagtag taaagtagga
    27841 aaagaagtac taaaaaactt aaaaatagat ctaataaagg atttaaacgt atataagaca
    27901 ccagagccat tcatatattt atcaataaat cgtatatctc cacaaaaaat attaattata
    27961 gaaaataaag acacttacat aacaataaca aaaatgctat tagaaggaaa agaaatactt
    28021 aaaaataaaa tagatactgt tatatacgga gaaggaaaaa aaataatcaa cagtattaaa
    28081 ggattaaagg aagatataac aattgaatat cttacaaata aagaaaatga atttttatat
    28141 tggggagata tagataaaac tggattatca atatacactt ccctaaaaga taccaatgaa
    28201 attaataata taagattatt tgaagttgct tatgagcata tgatagataa aacaaaggaa
    28261 aataccttga ggaatatccc caaaaatcaa aaagataatt ttgaatgtgg attagaaaat
    28321 attaataacc taaacttaaa agaaagtatt aaaaatattt tagtacaagg aaaatacata
    28381 cctcaagaag ctctaaatag attcgacttg gaggaatata actaatggaa atgattaata
    28441 ttttagaagg ctacaataag agagtaaact gtataggatc ttattatctt attgcaactc
    28501 ttgctaacaa tagaaataaa tttaaagaat ttgacaacat tcaattttac aatttattaa
    28561 ttcaagtttt gtgttatata tttgatagat ctcttagaag aaagaactgt ttaagagatg
    28621 atattaagga ttttattgaa gaaataaatc gtatggatta taaaataatg ctttcagagg
    28681 atgacttaaa ggatttagca aactatataa ttaatggatt aaccaatagt ggtaaagtat
    28741 atctatttac ctactattct ttagaacaag aaaaacatat agatgaaagt attaaaatta
    28801 tagaagataa aaatgtaaaa ataaataatc aggaaagact atcctactcc ttaacaacag
    28861 agggttatag attgttgtta tcaacaaaag aatatgatga actattccaa attcaaatat
    28921 cacaaatgat tgcaaagtta aggatagaaa agggtgatta tcaaggcgca aaggaagata
    28981 tttacgatat aataaatacc ttagagattc atcaacagaa aattgataat tatataaaaa
    29041 taataaaagt aaatatacta tatataaaag aagaaaaata ttcaacagta atagatacaa
    29101 ctgtagaaac acttttagaa gaaatggaca aatatgaaga attaaaaaaa gaagtaaaac
    29161 tattaatcaa agataaagag gactacttgg aaaaggatga ggatatagac aaagaaaaga
    29221 taaaaaatat aagaactcaa ctattacaaa tgaaagaatt aatagatgga atagttttag
    29281 caaagtctac agccacaaat ttgttatcta gagttcaaga ttttagagaa gaatttaaag
    29341 aaatacttaa aaaactatta acaactccag taattaaaag attcaatttc aaggaagaaa
    29401 tcttagatag acttgaaaaa gacagtgatt ttttattgat aataagagaa ttatatatat
    29461 ctttatttaa agtagagttg ccaaatcgat ttaatattgg agtggcatat caagaacaga
    29521 aactattaaa tactagagaa attgaaaatg aaatagttaa taaagacgag ttagaaatta
    29581 ttgaaaataa agagaaggag gaacgtataa attattgtga aaattactat tttgattttt
    29641 ttaaatactt aattaaatat ggacttgaaa aaggacaatt tacattagaa gaatttctaa
    29701 aaaattatct gaaatatgac aaagttattt acttagaaat aactaaagac tccaaattga
    29761 tgagagatat aataatttac ttcgctaatc aaagggaaag tatcaatatc aaaaagataa
    29821 tcagaatgtt agaaaatagt gccttctcac caaatacaga ttttcaaatt gaaagattaa
    29881 tagaacgttt attagtagaa gattttgaac tattaaatag cgttagccat attaaaagta
    29941 ataaaatatt tgaagatgaa atgaaaataa atactgaaat aaatttagaa actatgactg
    30001 cagttaagct agaaatagca aatataaaat ttgaaataat ctaaatataa ggggaaatac
    30061 aatgaatata tctttaaatg actataaaat agccaatgaa atattttata gattacttaa
    30121 aaaaagtgag ttaaaagaaa aagaagctaa agaattattt ttagcttata taaataacag
    30181 taatgtaagg tatgctttag aggcaatttg tgaagtatct aaaataaaaa taatattaat
    30241 ttcagatacc ttatacttaa taccaaataa tgacaatgat atttttgggt ttgactatag
    30301 aagagaatct ttattaggga aagatttaaa agatagttat ttaggatatt tgataatgac
    30361 aataatattt tctgaattta caaatggggt agcaccagca tcatatattc aagtattaaa
    30421 cttactggat ttagtacaag aatccttaga acgttcaata gctcgtgata atctagaaga
    30481 aatagaaaaa tcatgtgagt ttaatataac tatcttaaag aactattggg aaagtaaaag
    30541 aaagtgggac gaatctagta agaaaggaat agaaacaaca tcagcagatt tccaaatagg
    30601 gtttattaga aaaataatta atttcttaaa aaatgaaggc ttaattgtac acattgagga
    30661 tgaagataag ataaaaccaa ctagaagatt tcttgatctt atgaataatt attttttaga
    30721 tgaagaaagg aaagtattaa tagaaaaact agttttaaga aaaggggaaa gtattaatgc
    30781 cagtaattaa taaatacaaa attataaatt ttaaatttgg tatgggacaa gaaagaatac
    30841 ttagcaacag aaccatggaa ctgtatggag aaaacttaca agtagaagca gaaaatacag
    30901 caggcaaaac tatgagtatt caaactatca tacaaagtat ttgtccacta tcggatgtag
    30961 ccaaaccgtt tactgcaata tataataaaa aagaaccatt atactcaatg actgaatggt
    31021 tgctagatga tggtaaaacg ttattattaa cgggaatagg atttgagaaa aaggctggat
    31081 catctaaaga agaagggaaa gaaaaaaagg acgatttata caaatacttt atgtttatta
    31141 tagaagaatc taaagaaatg gaggttgata tagataatat acctttatta aaaaacaatg
    31201 acaaaggtat aaggatagta caacctttaa cggcctcaca agactatata aaaatgctaa
    31261 gagataaata tggaaaaaac aaagtacact tctttaataa ttacttaaga aaacaacata
    31321 gagaaaaatt agaagaatat ggaattaacc aaagtgaatg gaaagaaatt attataaaac
    31381 ttaatagtaa taacaaagaa ggtggactat cagactttgt taaagaacat aatacttcag
    31441 aaaaacttat aagagataaa atattacctt taatagagac aagcttaaca acagaagaat
    31501 tcgctccaat attgcaaatt aaggaacaag taagaaagtg tataaataat attatagaca
    31561 tgaatgaaaa attatcagat tataaaaact atagagattt aaacattaga gttgaaagtt
    31621 tacaaaataa aataaatagg atcctattca tagaagaaga taaaaataat gtattaggag
    31681 aattagctaa tctatatgta catatagaaa ataaacgcaa tgaattaaat gaagaaataa
    31741 aacagtatca aaaaatgaaa gcatcaaaag aagaggaatt taagatagta gaatatgaag
    31801 aagatagcta tgagtatttt agaaaagaaa aagagataca agatactcag gtagaatttg
    31861 ataaagccaa aaatgaatta aatgataaaa tagaagaact agaattagat gaacaatatt
    31921 taaatatatc aaagttcaaa agaaaaaaga aggggctaga agaaggggaa aaagataaga
    31981 tagaaaaaga aagtgaaaaa caaagaaaag aggtaaatca agaagaaata acggctatta
    32041 taaatcgtct aggtagcagt attcattatt gtttaaagga agaaagtatt ataaataatg
    32101 aaaaacttaa agaaaataca aacaacaata tagctataca agaaaaaaag aatcaactta
    32161 atgatgacaa aagtaagctc acaaaagtat tgataagtaa aaattcagaa attaaatata
    32221 tagaaggcaa agaacgtgat tttaataatc aactacatga atttaaactt aataacactg
    32281 atattaaagg ctttatttct aataattttt taggcgaagt aatagattat actaggtata
    32341 taaattattt aaatataaaa gtaaaatcaa tagaagaaaa tcttgaatcg aaagtcatgc
    32401 aaatagaaga atcgaataaa cttaaggatg atataaatga agctataaat aatcttaaat
    32461 tagataatca tgaatcttat atgaaactgc aagtattaaa aactagaaag aaagatatag
    32521 ataattttaa agaaaaatat aaaatcatta aaacaagatt tgaaatagac aatgaattag
    32581 ataactctac taaactatat aaagaggtaa aacaagagct taacaaaatg aaacttcaag
    32641 tacaggactt agaaaataat aaaaagcaat tagaaaaaga gctagaagat ttacaaaatt
    32701 ataaagatat aattttcgaa gaagaaatat taacagatct agaaagaaat ggaataaaac
    32761 ctattgaagg catgaaatat ctattaacct tagatggaga tttagaatac aaaaataatc
    32821 tcatatctaa aaatccattg ctaccatatt ctataataat tacaaataaa gagtatcaaa
    32881 aactgaagca aataaactta aaaaataaaa ctaaaatctc aatgcctata tttgtaagag
    32941 ataacataga agatactttt ataactacat ctagtaacct tgtaattagt aatggattaa
    33001 atatactatc ttctttcgat aacaaagctt tagatcctga aaaaagagaa aaggatatga
    33061 atctaatcaa agaacaaata aatagtattc gagataaagt ggagcaatat aaagcagaaa
    33121 ttgaaatatt agaagaattt aaacttctaa ctaataaaat tgattttaca gctgaagact
    33181 tatccataga tgtgaaaatt gaaaattata aaaaaattat atttgaaata aacaataaaa
    33241 ttaagagtaa tgaaaataat gagaagaatt tatctaataa aattaaagaa ctttacgaaa
    33301 ataaagaaaa gctattaaat aacaagaata tattatttaa taaattgaag gacattagaa
    33361 atatacaaga tacatattct ataattttag aaaataaaaa tgaaataact aagttaaaaa
    33421 caagtattga aaaaatcgaa gaagaaaaaa aagttattaa aaaatcaata gaagatattg
    33481 atgttaatat atttaattta aattcagaaa aagataggat aaataggcat atagatgata
    33541 cagaaagcaa gataaataaa tttaagattt atgaacctat taaacttgaa aatgctaaac
    33601 tagaagaatt agaaataaaa tacaagaaat acttagaaga ccctacaaaa aaagagattg
    33661 aaacattaaa ttatgaaatc tcagtttgct taaaaagaat agagagtatt actatagagt
    33721 tagaagaatt tagacaactt attagtagtg ataattttga aaatatacct atattattca
    33781 caatagaaga acttaataaa agaatagttt taaataaaag taaggttgat gaactacgaa
    33841 aacttaaata tagtattaaa agcgacatag ataaaaatca aggttcatta aacactaaga
    33901 gggaggagat acttaaaaaa tttaatggaa aagaaccttt atctactagc ttaatagtga
    33961 actttaattt tgaagaaaga aaaatagata taaaaaaaga tataaaggat attggtgaaa
    34021 ttatagaagg taatagaact acttgtcaaa atctagaaca ggaattaaga gacttaaaga
    34081 aatataaagt agaaagaaaa gctataacct atcaacctga agatattttt attgaaagta
    34141 aaaaactaca atcaaaactg caaaatgttg aatataattt aattaaatct ataaatagct
    34201 ataaaaatac aatattagaa ataaaagaat ttaattataa gcacatggaa aaatacaaaa
    34261 actttataga aactttaaat gtaaataaag ataattactc tcgacaaaaa gaacaagtag
    34321 aaatagtatt actagttata agagaagaaa taagcaaatt agaaaaacat agcgaacaac
    34381 taagaaaaga aaagactata atatcaaggc aaataaaaga ttatgttatg gactgtattg
    34441 atgaatttaa aatcataaat aaacttggta aacataaagg acaagcctta ttcaacatat
    34501 cactacctaa atctgataaa atagaaaata gtttgagctt agtagatatg ctaatggatg
    34561 aaattgtaga agaagcaaac ctaggtgatg ttgaacaaaa aataaatacc ttttatattt
    34621 taaataagac tcttaatata gggagaattc ctgtatcagt tataaaatat gaaataaata
    34681 gaaatgaaac tataaagtgg aactctatta ataacgaaac aacaggagga cagagatttt
    34741 gtatctcatt tattgtgaca atattgttga tggaatataa aagatatgat agaaatgcaa
    34801 tagttgatga aagtaaatat aagggtaagg tactacttat ggataatcct tttggagaaa
    34861 cgtcgcagca agatttcctt aaagaaatat ttgaacttgc taaaaaattc aacgttcaaa
    34921 ttatatccta tactcatgta acaaatgctt ctataagagc aatgtttaat aagatttatt
    34981 taatgacagt tgaaaaaact acttctaata aagaatttgt ggatattaat gagattaagc
    35041 aaaataaaac tgatgaattg gtgtatatga gtaaatttta tataggtaat aaagaagaat
    35101 taattcaaga ggatatattt agtttaatat aaataacaag taaacaaata aagtgattac
    35161 tatcagtgag gcaggtagaa actgaattac tttgtgactc attacgactc ttggggtaaa
    35221 attgtgtata aatgtgaaga atttcgtgat tgtcagtaca aaagcagctt tattaagggt
    35281 aataatagta agactcctct tgaaaatagg actaaaaact tacaagtatc taagttattt
    35341 catgaaaaaa gaaaaattaa agagaattat aagaccagag ggatgtgaac ttagagtgaa
    35401 tagaagtata caagtagaaa gagctatcgc agaacaaatt taattttatc atacgatagc
    35461 ctcttgttat tttagtgatg aaatccatca agcataccat aactacagaa aaagaggctt
    35521 ttctgatggt atattgggtt cttttagagt gtgtgatttg ctacatgttc attaggataa
    35581 taatagtcag taaatgatat tactgataga atagatataa taaactaatc tagttccata
    35641 ttgtaatgtt tattaaagag tatactttta tgattataat agctattttc tttgaagctt
    35701 attgtacaag agatttgaat atatcaaatt tggatatagg tataattctc ttagcagttt
    35761 tatttataca gtctttatct aaattagtgt tgtatatact ttgataaaag ggggatatgt
    35821 aactaagttg taatattgat gaaaataaaa attaaaaaat atagagaaaa actccttatg
    35881 atacaaaatg aacttgctaa atcagttggg gtaagacgag agactattgt acacttagaa
    35941 aatggattaa tgtaccataa aggtatccaa gataaatagt taaaaaacta gataagaata
    36001 aggagccttt acagaacaaa tttagtgtgc tcgtgcgata gccctttttt tataaaaatt
    36061 aatctaataa cctatataat taaatttata aaacatatat gaaagggtaa aactatatgg
    36121 ctaaaattga taatatgatg tccatattat ggatgttaaa ctcagacaaa aaagttaccg
    36181 caaaacaaat atctgaaaaa ctagaaataa atataagaac tgtctacaga cacattgatg
    36241 cattatctgc tagtggagta cctataattt cagatacagg tcataatggt ggatatacac
    36301 ttttgaataa tttcatcaaa gcacctcttt ttttcgatac agaagaacaa actgcgttac
    36361 ttcatgcagc tatatttgct aaagaagagg gaagtttctt agatgacaca ctagacaaag
    36421 caacctcaaa gttaaaaatg tattcaaatc aaaaacaaga agaaacttta acaaaacatt
    36481 taataggttt tgaagtaata aaaccaactg gaaaaatatc catagaatct gtacttaaaa
    36541 agttaaaaaa tagtatagca aatgaattat ctgtagaaat caactatcgt acagctcatg
    36601 aagaaaaatc aaaatgtagg attataaatc catatggaat tgtacgttgg aataataatt
    36661 ggtacatagt agcattttgt caaatgagaa atgagattcg tagttttagg gtagatagaa
    36721 ttagtagtgt catagaaaca tcaaatactt ttaataggcc aaaagaattt tcagcaagtg
    36781 aattttttat aagagggatt atggatgaaa caaaagatga ggataatctt atatcattag
    36841 ttatagaagg aaagatgaat gcattggata acttatgtca gcattggctt ctaggatatt
    36901 atttaaaaga aaggacttca aataaagcaa tatttaaact tcaagaagag ataatattta
    36961 catatgtacc gaatatactt ataccttata gaaaatctat acaaataatt gaaccattaa
    37021 gccttaggga taaaatggtt gaagatttac tcgaattaat taatcattat caaatttaat
    37081 aacttaactg acagagtatg tcagttaagt tattttacaa ttaggtatat taattaatta
    37141 tagaaggagc aaaaaaatga aaaagagaaa agtatatatg tatgtattcg atactatgtc
    37201 agattgggaa gttggatatc taactgctga attaaataca ggaagatatt ttaaaaaagg
    37261 attaaaacca ctaaaagtaa ttacagtagg agttgataaa aatcctatta taacaatggg
    37321 tggattaaaa gttttacctg agattacaat tgacgagctt aatattgaaa gtaatgactt
    37381 attaattttg ccagggggaa atacttggac atctataggt catgagaaaa tattagaaaa
    37441 atctaaagag gctttggaac aaaggtctat agttgcagct atttgtggtg ctactttagg
    37501 gttagctaaa acaggtttac tagattttag aaatcataca agtaatgatt tagaattttt
    37561 aaaaatggtg atactaagtt attctggaga aaaatattat aaaatggaat ctgcggttaa
    37621 tgatgaaaat ttaattactg cttctggaat tgcaccatta gaatttacag tacaagtttt
    37681 aaaagtttta gatgttttta aagaagaagc attaagttct tggttgaacc tttataagac
    37741 gcatgactct aaatattttt tcgaactaat gaattgtaat aaataaattt aagatttaat
    37801 tttaaattca aattttaaat tactttttct atggcaatag ataagtagtt tagaatttta
    37861 tcatatatat aatttgtatt tataccaaca caaatagaaa tgatagttcc aataataaaa
    37921 tttataatta gttccatatg gagtacattt ctttcctact aatataacta agaatatatg
    37981 aaaccaccat agattgtagt tttttctgtt gtattatatc atgggatagt aaagtgacgt
    38041 atatttttga tgaaaaccta ggattgtata tgaaatgaat taatcctgtg tattaatctt
    38101 gagattcgtg tctaatgatt tgtaaaaata aataatgaaa tacttagaaa tacttgtaaa
    38161 tgctagtcaa tattggctag tattttttat tttagtaatg aaatccccaa ctgtacttca
    38221 tccacaaaaa aagagatatt tttgagggtg tattggagtg aacttaggct gtataattat
    38281 acatttcaat atcttatatt aattaggata atagttggta aatgatatga ttgaaacaat
    38341 agatataata aactaatcta gtactgtact gcttactaaa gaatatattt ttatggtata
    38401 agaatttttt agagcgttga ttctatagga gtgaataaaa aagtaatttt ataatattat
    38461 ttgtaaccat tagatgtcta ttatgaaatc ttttatggac tatatttgta aaaatattat
    38521 taagtattta caatattttc ttgcaattta tttggaaagg ttatatattt ataacatgaa
    38581 gtgttataaa tatataacct ttaaggtgga ttttatatgt ctaaatcaga aactggtgta
    38641 aaatcaaaac tcatattgga attacttgta ataacttatc tttttatagc tgtattagct
    38701 actaagtata tttttaaagg tccaagtact gcatctcttc caggactgag ctggttaata
    38761 gtctttggat gctattcaat ttataataaa aaatataatg atttagttga tgaatcatct
    38821 aaatttatac tatcaaaggt taatcagatt gcaataacaa taatgatgta ttctatagtt
    38881 ataatagcta ttttctttgc aacaccttat ataagtaata taaatatatc aaacttggat
    38941 actggtataa ttcttattgc agttttattt atacaatcgt tatctaaatt aattttgtat
    39001 atatactttg ataaaagggg gatatgtaac taagtggcaa tatcgatgaa aacaaaaatt
    39061 aaagaatata gagaaaaact ccttatgaca caaaacgaac ttgctaaatc agttggggta
    39121 agacgtgaga ctattgtaca cttagaaaat ggaaaatata atccttcttt aaagttagcg
    39181 atggatattg ctaaagtatt cgggaccaca gtagaaaatt tgtttgaatt tattgaagaa
    39241 gattagtaga taggatataa atatttataa gattgttaat aaaatatagt tatttaatta
    39301 ttaattttgt taaaaatatt tataaataat ataatgtatg agtaaagtta ggttgtaatt
    39361 attttaagat aagaagacta ctgtaaatga taaaaattag tgaatataca gccaattaat
    39421 aaataataga taaaacttta aagcttagat gtgaggatga gatatggtct tacagtagtt
    39481 cacactttta attaagactt aaaatggaaa ccatacacat acatacacta tttactgaaa
    39541 gtggactaga taaaaaaata ataagtgatt taagaaagta tctcatatac tatataagta
    39601 ttattttagg tgacctattt ataggtcatt ttttatgtat ataaatgata ataatactaa
    39661 tatttaaaat aggttttaaa gaaataaaat atccagattg aaaaatggat taatgttaat
    39721 aaaaaaatta tatgtataaa aataggtata attatagaaa aaaacaataa taaaataagc
    39781 tgttatagaa aaatacaatt atatagattg ttgttgaatc attgactgca agaataataa
    39841 catttaaaat gaacatggag aatatggggg aggaatcaat atgaagagaa aaatagcagt
    39901 tgttttatta actgtagcaa tgttattatc acttgtagct tgtagcaatg ataacacatc
    39961 aaaaagtgat aaattagagg atacattagt agtatatact acacatggag aggatatgct
    40021 aggagcaatt tctgaagaat ttacaaagga aacaggagtt aatgttgaat ttataaactt
    40081 aaaaggtgaa ctagcagacc gtgttagaag tgaaaaagat aatccgcaag cagatataat
    40141 gtttggtgga gatacatcta catacatgca attaaaagaa gaaggattat atgaaaagac
    40201 tgaaccaagc tggaataatg aattagacaa gtcttacaaa gattcagatg gatattggta
    40261 tggaactata aaaacaccag taatgatgtt ttataacaca aaactattat cagctgataa
    40321 agcgccaaaa gattggagtg atttaacaaa agaagaatac aaagacttaa tagttactag
    40381 agattcatta tcatcatcta tgcgttctac tattagtaac ttaatcaatt actactcaaa
    40441 tactaagagt gaagaagaag cttggaagta tcttgaggat ttaaataaga atataaagaa
    40501 ttactacaat agtggaagtg tcatgtattc agcaataggt aaaggtgaag ctacaatatc
    40561 tgttgcagta ttaagtgata taatagagaa taaagaaaaa aataatatgc cattagaagt
    40621 tgtagatgca gagagcggtt ctgtagtaat cacagactgt gtagcagctc taaaaaatgc
    40681 accacatcca aatgcagcag ctaaatttgt agattttgtt ggaagtgcta aggttcaagc
    40741 tatggttgca aatgatttta atcgtattcc aacattagat tctgcactta aagattcacc
    40801 aaagtggatg tcaaaagaat ataaagctat ggatgtagat tggtctgtaa ttggaaaaaa
    40861 tcagtctaac tggattgaaa agtgggaaac agatataata gataacaata aaactataaa
    40921 atagcctaat taaataattt aatattatat acaaaaatag tgaggatttt cctcgctatt
    40981 ttacgtataa ggaaactata gtatctcata attttgtata attcatacaa caaaatgata
    41041 ctaatacata cttattaaat aaaatagttt ttgagtaact gatgaagtaa ttgactatat
    41101 aaagtgattt gtagacatag tctctcaaat aaagtgaatt ttttattatg caaaactttt
    41161 aattatagta aaagtacaat ttatatgggc aaggagataa aaatggaact aaaaattagt
    41221 gattataaat tattatgtaa taaaggtagt ggcgctttta acgaagatat cgtaggttta
    41281 tcaccttatg gggcatgggt acttgatgga gctacaggtt taaataataa aaatttagtc
    41341 tctacagaaa gtgatgccaa atggtatgta agttggtgga atagttatct tcatgagaat
    41401 atggaaagag aagatagctt aaaagtaatt attaaagaag gcattgagga gataacaaaa
    41461 gagtactaca aattaacaga tgataaagaa gtagaagcaa tagatattcc ttcttgttca
    41521 tgttctatat taaagtttta taaagataag atagagtatt tacttttagg agattgtact
    41581 ttattagtta acaataatga atctgtgaaa attttaaaag atgagacttt atgtgaactt
    41641 gataaaaaag tatttgattt tatgaaagaa gccaaagatg ctacaaacct tactttaaag
    41701 gagagaaaag ccgaggtaat gcgattataa tagagaatag acttaagaaa aatactgtgg
    41761 ggggatattg gactttggaa ttttctaaag aggccgttga caattgtgta agtggattta
    41821 ttgatataga atataaaatg aaaattatga tggctagtga tggtttttca tgtggatatg
    41881 atagatataa gatttttgat aaaaatgaaa tgatgaaaat agcagagatt aaaggtatag
    41941 attatatata tagaaaggtt agggaactag aaaaggatga tgaaatggca atagagtttc
    42001 ctagatttaa aatctatgat gattcatcat gtgtatattt agagatttat aaatattaga
    42061 taatttatgg agacgatatg aagaatatag aattaaaaga aattaatcat tattatggaa
    42121 aagaaaaagt attacatgat attaatatat ctattaagga aggagagttt tttactttac
    42181 taggtccttc tggatgtgga aaaacgacta ttttaagaat aattggtgga tttataaaac
    42241 cttcttctgg aagtatttat gttggtgata aaaacattac caatttagaa ccagaaaata
    42301 gaaatatggg aactgttttt caaaactatg cactgtttcc aaatatgaca gttgaagaaa
    42361 atgtatctta tggattgaaa ataaaaaagc tgtctaaaaa aattataaag gaaaaatgtg
    42421 ataattattt agagctgtca ggaatgaaag attttaggaa aaaaaagatt gatgaattat
    42481 ctggtggaca gcaacaaagg gtggcagtag caaggtcatt agcgactgag cctactatgc
    42541 ttttattaga tgagccaatg tctaatctag atatagcttt aagaatcaag atgagagaag
    42601 aaataagaga gatacagcaa aagacaggta ttactacttt atttattacc catgatcaac
    42661 aagaagcctt agctatttct gatagaatcg ctgtaatgga taagggtaaa gttcttcaaa
    42721 ttggtgtgcc aatagaagtt tacaaaagcc cattgaatga ttttgtagca aactttgtag
    42781 gtacttcaaa ctgtattgga aaagaagatt atgttaattt taatattgaa aaagagacaa
    42841 agccttatat ttataaaaga cctgaagaaa tgatattatt gcaaaataca aatcaaagtg
    42901 gttttgtaaa agtaaaagta gaatctaaga attttctagg agcaatttta gaatatacag
    42961 tatcaaatga tgggaaaaaa tatcaagtaa ctgagcttaa tagactgaat aatagcaata
    43021 aattaaacgt aggggatatg gcatatttag gtgtgctaca aggtgactaa tatgattaaa
    43081 aaaataaacc gtagtaaatt tacatctaaa aatgttattt ttattgtttt atacagtata
    43141 ttattttgga ttttgattgg atatattatc ataccaatag gcaatacttt tgtacaagca
    43201 ttccaaggta aaggtggata ttcttttgaa gtttttaaag aatatttttc aaatactaat
    43261 aatttaaggg tagtaagcaa tactataatt ttgggattgg gttctgtgtt cgtatgtgga
    43321 attatgggaa tatcacttgc cttatatatg acatttgttt gtgataaaca taagaaaatc
    43381 atacatattc ttttactaag tccaatgatg attcctggag taattatggt aattgcatgt
    43441 attcagattt atggagaaag tggaatagtt acaaaatcta tagaaatttt actccccttt
    43501 atcaaaaaac cttatgattt tagtggatta aagggaattt tatttgtggt tgcttatact
    43561 cagtatgtat atttttattt aaatgtatat gttgccttaa aatatgtgga ttactcaaca
    43621 gttgaagctg caaaaggaat gggagcatca aagctaagag tattttttga tgtaatatgg
    43681 cctgttatta cacctgctgt tataacttct actattataa cttttgcatc agggattagt
    43741 tctttctcag ctccaaatct tataggtggt ggttttaaag ttttaagcac tcaaattgtt
    43801 aggtctaagg ctaataacca tatggatata gcttcagttc aagtaattac tttattacta
    43861 atggggattt tatttatgat tttattacag tattatggca aaaaatatgg agtagtgcca
    43921 tccttaaaat ctcaaagtta taaagttcat aatagaaaaa aagacttatt tacatttgta
    43981 tgtaaggtta ttattgaaat acaaattatt atgattatgc ttcctatagt tactattgtg
    44041 tatttatcct ttgtaagcac acattcaatt atgacaacca tatttcctag agactttaca
    44101 ctggaaaact atcagatgat tttccaaaaa tcacgtgtat tacagcctat attaaatagt
    44161 ttcaaaatgt ctttaatggc agtagcagta ggattgataa ttactgtacc agtatcatat
    44221 ttagtaatta aaaaggatac tgcatataat cgactagcaa aatttattat aatgttacct
    44281 tggagtatgc ctgcaagcgc cattgcagtt aacctaatta atacatttaa tcaaaaaagt
    44341 atatttgctt ttaatgaatc cttaatagga ggtttttata ttgtacctat agcatataca
    44401 atttatgctc taccattatt attttcatcc aatgaggttg ctatgaagtc aataaattta
    44461 ggactagaag aatcatcaag gagtttaggt gctggaagga taaaaacctt ttataatgta
    44521 atagttccaa atatgatgcc aggaataatt tcaggagcta tattagtatt cattcgtaca
    44581 attggggaat atacaatgtc agccttgctt tatggtgttt ataatagacc tatttcaatt
    44641 tctattgtaa ctaatatgca agaatttaag ataggagtat ctatggctta tggtgtatta
    44701 gtaattggta tatgctatat agcattagga ataattttta aattagatag aaaaagattt
    44761 acataagata acaaaggcta atttataaat tggtgcttag aaaaccaaat cactttatta
    44821 gctaaagatg aatttgaagg gtgacacttt ggtgttttat gtaaagttta tggaagtaaa
    44881 acattaaata atcgtgagta tgattcctat aaatcgtata tactattgaa atccaagaaa
    44941 aggtattggc taaatagcta atatcttttt aattttttga ctcccatata gcattaacta
    45001 ttacatgagt atctttaaat ttatagttaa agtatcttaa ttgtttatta taaaaatata
    45061 ggatggctaa agcacattaa attaaaacac ttaaaaagag aagatgtaca tgagaaatat
    45121 aattaactat gatacagact tctaaaaatt atttagtgaa aaggaactta gagtgtataa
    45181 taaaagttcc ttttcaataa ggaaaacgat tgttattttc tgagcttcat taccctgata
    45241 ttatttaaat ataaacaaca acaaaattaa caagaagtaa attcaaccta aaatttaaaa
    45301 tatcgggagg tacaatatgt taatcaatat gaaggaaatg ctaaaagtgg cacaagaaaa
    45361 tcaatttgct gtaccagcat ttaatatagg aagtgggcaa atacttaaag cagtagtaca
    45421 gggtgctaac gaaaaaaatg caccagtaat acttgcaata cacccaaatg agctaagttt
    45481 tctaggcgat agctttgtag ttagttgcat agaagaagca aataagagta aagtacctat
    45541 ggtaatacac ttagatcatg gggaaaataa agaacaaata ttaagggcaa taagatgtgg
    45601 atttacatca gtaatgattg atggctcaca tttaccatat gaagagaatg tagctatatc
    45661 tagggaaata gtagaaatag caaaaggatt aaatgtgtcc gtagaaggag aacttggaac
    45721 aataggaaca acaggaacat caagtgaagg tggtactgat gaaataatat atacagattc
    45781 aaaattagct aaggattttg ttgaaaaaac aggagtagac acactagcta ttgcaattgg
    45841 tacagcacat ggtatatatc caaaaggttt taaaccagag cttaaactag atttattaaa
    45901 agaaataaga gaagtagtag atataccttt agttcttcat ggtggttcat caaatccaga
    45961 tgaagaaata gcacaagcag ttaaactagg agtttgtaaa ataaatattt cgagtgatgt
    46021 taagtcggca tattataaga agtgcagaga actattagaa caaaacccaa gcttatatga
    46081 gccagatact atatatccac catgtataaa agctgcaaga gaagttatag agtttaaaat
    46141 gaatctattc aatgctatag ataagttaaa gtgcttttat aaaaataaaa attaattatg
    46201 aggtaacaaa ttaaaaggag gggagaattt atgataggta aaatactacg aagcgattgt
    46261 attgtactag ataaacaatt acaaacaaaa aaagatgtaa ttaattatat ggcaaatcat
    46321 ttgtaccatg gaaattatat aaataataaa gaaaagttta tagctgatat taataaacgt
    46381 gaaaaagaat catcaactaa tatgggtaat ttaatagcta tacctcatgc taagtcaaat
    46441 gtagtaaatc aacctgtaat gatgtattta agaacagata gagagattac agataatgat
    46501 ggtcaaaaac ttaggtcatt ttttatgata gcagttccag aatcaggttc agatgaacat
    46561 cttaagatta tatctaaact agctacatat ctaatggaag aaaattttct taagcatcta
    46621 ctaagtgcag aaactccata cgaaatatta agtatattta ataataaagc tgcagaatca
    46681 ggagatttta gtaataatca aaataaaggt cttatagttg ggataacagg atgtccaaca
    46741 ggtatagctc atacttttat ggcaaaaaaa gcattagagg aagcagggca agaacttgga
    46801 tatgaagtta aaatagaaac tcatggagct ataggtgtag aaaataagtt gactaaagaa
    46861 gatataaaaa gagctgaata cgtaataata gcttgtgata aagaagttca actgagtaga
    46921 tttataggaa agaaagtgct acaaaagaaa gtagtagatg ctaagaatgt ttcattagct
    46981 aaaaatttaa ttataaacgc tgaaaatgga atggctaaag aaattaaacc aggtggaaag
    47041 actttagaag attcaaaaga aatgcttcaa gggtcagaga caaagggtgt ttataagcac
    47101 ttaatgagtg gtgtatctga aatgattcca tttgtagtga taggtggaat tggtatagcc
    47161 atagcattta tgtttggtat atttgcatca gacccaactc atgaaacata caaccctata
    47221 gctggattct ttggacaact tggaggcgat tcagcattta agttatttat accgatactt
    47281 gctggtttta tagctaaatc aatagcagga agacaaggat ttcctcctgc tatgatagct
    47341 ggttttatgg cagttattgg aggttcaggg tttataggtg gtatggtagc tggttttgca
    47401 acaggatata tagctttagg agtaatgaag ataacagaaa atttaccaga tgtattaaaa
    47461 ggagtaaatg ctgtattgat atgtccactt ttgattgcat ttacgagtgg agctataatg
    47521 ttctttgtag ttaatacgcc agcatcatgg ttaaatgaag ctttacaatc ttggttacaa
    47581 agtatgtctg gaactaataa agtattatta ggtgcaatat tagcagggat gatggcatca
    47641 gatatgggag gacctattaa taaaacagct tcagcttttg gattggctat gttttcaagt
    47701 aatatatttg agccatcagc agcattaatg gtaggaggaa tggtacctcc attagcgata
    47761 tcattagcta tgttaatatt caaaaataag tttactaaag aagaaagaaa gtcatctcta
    47821 ccaactgcta taatgggggc atctttcata acagaaggcg caataccgtt tgcagctgca
    47881 gacccattaa gaatgattcc agcaaatata attggttctt gtataggtgg ggcaatctgt
    47941 atgagtttag atataacttt aatggcacca catgggggaa tatttgtaat accatttgca
    48001 tgtagtgcac cagttgcata tatagcttgt atattgattg gtacagtaat aacagcattg
    48061 attatagggg tagcaaagcc tactataaga agtgaaaaca gtgaagttga attacttggt
    48121 gcttaaatta taaataatag taaaaataag taatggaata acaacaagta tatagaataa
    48181 taataaggat atttagaatc agttaactac ttctaaatat ccttaatttt tattttgaaa
    48241 ttatattatc tattatatca atttagtaag gtgtatcata aattctttta tgatacagaa
    48301 gtatcttagt aattttacat agttagtaaa tatgttaaaa tagtacttaa tataaacata
    48361 gaaatatata aacataggta gggagggcta agtatgcttg tgtcgaggca aattgaattt
    48421 cttaaattga tactaaacga aaataaatat agacctataa aatactttaa agataaatta
    48481 aatgtatctg ataaaacttt acaaaaagat ttgaagatga ttgagaaata tttagaaact
    48541 tttaatataa aaattgatat aaaaagaggc tatggaatat taatagaaga tttagcaaag
    48601 aagaacatag acttaattaa tagtttaaat atacaatcga aggaaaataa atctttatca
    48661 gtagagcaga gaagaatgga gattttaaag tatttattac taaattcaaa taatataact
    48721 tcaataaatc agttagcaga taaatattat gtaagtaaaa cttctatagt aaatgatttt
    48781 aaatatatag aggcatggat aaaagaatac aatttaaaac ttaataaaac tttagaagga
    48841 actaagatta taggtaaaga agtggatata aggaaatcga ttgccaaaat gatggatgat
    48901 ttattagatg aaaattatga acaacatgat atatctgagt taacaagatt agattctgct
    48961 acatttagtg cattgataaa tttatttgat attgattcta ttatatttgt agaaactata
    49021 attacagaat tagaatacaa tttaggatat actataaatc agccctacta tataaattta
    49081 attacccata tacttatatg tttaaagaga atcgaagaag gtaatcaaat agaatctcag
    49141 gaagaaagag aaatttctgt agataatttg gacgagttag tatataaaaa tgtcactaca
    49201 cttatagata aaatagaaaa tagatacaga gtaaaaataa cgaaagatga aattatgtac
    49261 atatatattt ttttggtatc atctggattt agtagcgaaa ataatagtag ttataatgat
    49321 gaatcagata tattaagtga aagtgaaaaa gtttcatcta taatgataga gaatatgtca
    49381 caatttttaa atataaatct caaaaatgat gacttactac aaaaaagtct agcatctcat
    49441 gtaaggccta tgttaaacag attaagatat gatatccaaa taaaaaaccc gttgttagga
    49501 gagatacaag agcgattttc agatgtgctg ggattatgta tgatgactat aaatattatg
    49561 acagagtatg ataatctgaa gaatattagt atagatgaag tttcgtatct tgccacatat
    49621 tttcaagcgg caatagaaag aaatatgagt agtaaaagag taatagtagt ttgtcataca
    49681 ggatatggaa catctcagct ccttgcagcc agattaaaaa gagaatttcc tgaatggact
    49741 atagttgata tagttcctat gcatcagttg agtaagcgaa atttagatga tgtagacttt
    49801 gtaatatcta ctgtaaactt agatttgaaa gataaattac atatagttgt atcagtacta
    49861 ttaatggaaa gtgatattaa taatataaaa aatgctttac tttctaaacc taaaaaaagt
    49921 atgaacttag atacaaactt attaggaatg tatttagaag ataatatata ttttaatttt
    49981 gatgtagctc agatggaaag tttaataggt gtgaatttag ggagtaaata tgaatctatt
    50041 tcattaggaa aagatttgaa tatatatata cataagcaaa gtaaattaaa taaagggata
    50101 atgaatatca ataatgttac tagaacgatt gatatttatt tagaaattag taatcaaagt
    50161 tttttaaaga atattttaat ggaaatatca aatttatata ggcaaaagaa ctatataaac
    50221 tatataatag attgcaagag caaagaggat attaaaatat tatttaagaa taatctattg
    50281 gagaaaacaa atggaaatta gagaaatatt aacagaagaa agcattattt tagatttgaa
    50341 atcaactaca aaagaaggca taatacagga attagtagat aaattatacg atacaggaag
    50401 tataacttta aaaagtaagt ttataaaggc attaaaacat agagaaaaaa taggctctac
    50461 tagtataggt tatcaaatgg ccatgccaca tggaaaaaca aaatatgtaa agaaggcatc
    50521 catagtaata ggtaaatcta aaaaaggtgt agattggcca agtatagatg gcttacctgt
    50581 aaaggtaata attatgtttg ctatgccaca atctggaggc aatgaagata tgatattaat
    50641 atctaaaata gcttacattt taggtggtaa aaatataagt gaaagacttt taaatgtaca
    50701 tacaaaaaaa gaaatattag atatatttta tgaagtgtag gtattgtata aattataaat
    50761 ttgaggtttt taaaataaaa tgtgtattct tttaaatagt atagattaat ttttagacac
    50821 tagagaggag aggatatcta tggcaatata tttagaaatg cctaaattaa tacatgaatt
    50881 tccttttcgt agtctatcaa atgaaggaga agtattaaca acaccacact ggcataagga
    50941 aatagaaata ttacttataa cagaaggtgt agtaaatcta tttataaatg acaagcctat
    51001 gcaattaaaa aaaggcgaga tagtgataat aaaaggagga gatattcatt atatactgcc
    51061 atcaccggga agtgaaagat tagtatttca atttgatata tcttttttta atgatttaat
    51121 tttattaaat gaggaaaaag aaagcttaat taatttattc agtagtataa aaaaatgtag
    51181 tagagattgg tcagcaaaaa taagaaagtc agttttcgat atattgatag atatctacaa
    51241 tgaagaccta tacaaaatag aaggatatag ttatgcaata aaaggtaaaa tgtataattt
    51301 aattcctata ttatatagac agattcctag agaaattgaa gaaaatacag tagaatatga
    51361 aataaactct aaagagatat tagagagatt gaacaatata ttcaaatatg tagaaaaaaa
    51421 ttataaacaa cctataaaat tagaagatgt gtcttatgaa gtaggattta gtgtatatta
    51481 ttttactaaa tttttcaaaa aaaatacagg aaaaacattt ataacttttt taaatgaata
    51541 tagattagaa aaagcaaagt ggatattact taataacaat atatccattg tagatttggt
    51601 tgaagaagtt ggttttggaa gtacaaaaac tttttacaga gtatttaaag aaaaaattgg
    51661 agtctctcca ttagaattta aaaaaagata taatagcttg gagaaaaaag ggggtgcctc
    51721 aaaatgaact tagttcatga ggcactttct tttatatgaa atcaaatata ttttcagtat
    51781 ttcaacaatg aaaattccat tcatttataa agtctaaatt ttagttctca atacaatctc
    51841 tatttcgaaa taaatccttt tatatatata aatatacagc aatatatgag aataagataa
    51901 caataatgag gatataaaaa tgtat
//
LOCUS       DAMJVX010000012        49362 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMJVX010000012 DAMJVX010000000
VERSION     DAMJVX010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.013"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            25..1305
                     /locus_tag="PC407_000629"
     CDS             25..1305
                     /locus_tag="PC407_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDF2496105.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKVDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            complement(1568..2959)
                     /locus_tag="PC407_000630"
     CDS             complement(1568..2959)
                     /locus_tag="PC407_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2496106.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(2970..4190)
                     /locus_tag="PC407_000631"
     CDS             complement(2970..4190)
                     /locus_tag="PC407_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDF2496107.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(4204..4992)
                     /locus_tag="PC407_000632"
     CDS             complement(4204..4992)
                     /locus_tag="PC407_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2496108.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(5009..6283)
                     /gene="larA"
                     /locus_tag="PC407_000633"
     CDS             complement(5009..6283)
                     /gene="larA"
                     /locus_tag="PC407_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HDF2496109.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(6401..7114)
                     /locus_tag="PC407_000634"
     CDS             complement(6401..7114)
                     /locus_tag="PC407_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2496110.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            7239..8498
                     /locus_tag="PC407_000635"
     CDS             7239..8498
                     /locus_tag="PC407_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HDF2496111.1"
                     /translation="MKKNKIIAFFAPAIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKHEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFIKKFSRKK"
     gene            8766..9332
                     /locus_tag="PC407_000636"
     CDS             8766..9332
                     /locus_tag="PC407_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2496112.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(9533..10738)
                     /locus_tag="PC407_000637"
     CDS             complement(9533..10738)
                     /locus_tag="PC407_000637"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2496113.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(10841..10947)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11039..11407)
                     /locus_tag="PC407_000638"
     CDS             complement(11039..11407)
                     /locus_tag="PC407_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HDF2496114.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(11459..12736)
                     /gene="obgE"
                     /locus_tag="PC407_000639"
     CDS             complement(11459..12736)
                     /gene="obgE"
                     /locus_tag="PC407_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDF2496115.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(12847..13137)
                     /gene="rpmA"
                     /locus_tag="PC407_000640"
     CDS             complement(12847..13137)
                     /gene="rpmA"
                     /locus_tag="PC407_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDF2496116.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(13151..13468)
                     /locus_tag="PC407_000641"
     CDS             complement(13151..13468)
                     /locus_tag="PC407_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDF2496117.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(13483..13794)
                     /gene="rplU"
                     /locus_tag="PC407_000642"
     CDS             complement(13483..13794)
                     /gene="rplU"
                     /locus_tag="PC407_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDF2496118.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(13895..15316)
                     /locus_tag="PC407_000643"
     CDS             complement(13895..15316)
                     /locus_tag="PC407_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HDF2496119.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLIIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(15334..16035)
                     /locus_tag="PC407_000644"
     CDS             complement(15334..16035)
                     /locus_tag="PC407_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HDF2496120.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(16037..17881)
                     /locus_tag="PC407_000645"
     CDS             complement(16037..17881)
                     /locus_tag="PC407_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HDF2496121.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFI
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            18200..19393
                     /locus_tag="PC407_000646"
     CDS             18200..19393
                     /locus_tag="PC407_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HDF2496122.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(19605..20687)
                     /locus_tag="PC407_000647"
     CDS             complement(19605..20687)
                     /locus_tag="PC407_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2496123.1"
                     /translation="MKKIRKLATKLLIATMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGESVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQEEAILDEVYGNNLRPKIERYEGVPCSLINSSINTIKFTSKNSNLDPS
                     RHTVSVIGFMNTPYGMVALAPDGVPVEYPFEYDKPFSLDFTGKNLSAAELSKKCKNNN
                     VIIKIEIGEIEPNSGRGQVRYARYVN"
     gene            complement(21251..22042)
                     /locus_tag="PC407_000648"
     CDS             complement(21251..22042)
                     /locus_tag="PC407_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496124.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTNIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(22046..22375)
                     /locus_tag="PC407_000649"
     CDS             complement(22046..22375)
                     /locus_tag="PC407_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2496125.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            complement(22860..23273)
                     /locus_tag="PC407_000650"
     CDS             complement(22860..23273)
                     /locus_tag="PC407_000650"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDF2496126.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(23314..24444)
                     /gene="rodA"
                     /locus_tag="PC407_000651"
     CDS             complement(23314..24444)
                     /gene="rodA"
                     /locus_tag="PC407_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDF2496127.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(24529..24813)
                     /gene="minE"
                     /locus_tag="PC407_000652"
     CDS             complement(24529..24813)
                     /gene="minE"
                     /locus_tag="PC407_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDF2496128.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(24831..25628)
                     /gene="minD"
                     /locus_tag="PC407_000653"
     CDS             complement(24831..25628)
                     /gene="minD"
                     /locus_tag="PC407_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDF2496129.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(25652..26335)
                     /locus_tag="PC407_000654"
     CDS             complement(25652..26335)
                     /locus_tag="PC407_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDF2496130.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYENESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(26433..29411)
                     /locus_tag="PC407_000655"
     CDS             complement(26433..29411)
                     /locus_tag="PC407_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDF2496131.1"
                     /translation="MEENKQVDRLKIIKIVMSVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMLTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKMNVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(29423..29911)
                     /gene="mreD"
                     /locus_tag="PC407_000656"
     CDS             complement(29423..29911)
                     /gene="mreD"
                     /locus_tag="PC407_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDF2496132.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(29926..30819)
                     /gene="mreC"
                     /locus_tag="PC407_000657"
     CDS             complement(29926..30819)
                     /gene="mreC"
                     /locus_tag="PC407_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDF2496133.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKDMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(30829..31839)
                     /locus_tag="PC407_000658"
     CDS             complement(30829..31839)
                     /locus_tag="PC407_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2496134.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(31960..32619)
                     /gene="radC"
                     /locus_tag="PC407_000659"
     CDS             complement(31960..32619)
                     /gene="radC"
                     /locus_tag="PC407_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDF2496135.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(32664..33248)
                     /gene="maf"
                     /locus_tag="PC407_000660"
     CDS             complement(32664..33248)
                     /gene="maf"
                     /locus_tag="PC407_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDF2496136.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(33468..34439)
                     /locus_tag="PC407_000661"
     CDS             complement(33468..34439)
                     /locus_tag="PC407_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HDF2496137.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(34455..35030)
                     /gene="rsxA"
                     /locus_tag="PC407_000662"
     CDS             complement(34455..35030)
                     /gene="rsxA"
                     /locus_tag="PC407_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDF2496138.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(35045..35638)
                     /locus_tag="PC407_000663"
     CDS             complement(35045..35638)
                     /locus_tag="PC407_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDF2496139.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(35651..36220)
                     /locus_tag="PC407_000664"
     CDS             complement(35651..36220)
                     /locus_tag="PC407_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HDF2496140.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQENNEL
                     RKAVLPEAKEFKEMDKSTYKDLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(36220..37197)
                     /locus_tag="PC407_000665"
     CDS             complement(36220..37197)
                     /locus_tag="PC407_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HDF2496141.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(37220..38557)
                     /gene="rsxC"
                     /locus_tag="PC407_000666"
     CDS             complement(37220..38557)
                     /gene="rsxC"
                     /locus_tag="PC407_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDF2496142.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(38773..39717)
                     /locus_tag="PC407_000667"
     CDS             complement(38773..39717)
                     /locus_tag="PC407_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496143.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(39828..41102)
                     /locus_tag="PC407_000668"
     CDS             complement(39828..41102)
                     /locus_tag="PC407_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2496144.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSNQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKSKNNGWYKVKLSSGTIGWVSASYISETNEGTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(41234..41605)
                     /locus_tag="PC407_000669"
     CDS             complement(41234..41605)
                     /locus_tag="PC407_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2496145.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(41833..42432)
                     /locus_tag="PC407_000670"
     CDS             complement(41833..42432)
                     /locus_tag="PC407_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496146.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(42633..42845)
                     /locus_tag="PC407_000671"
     CDS             complement(42633..42845)
                     /locus_tag="PC407_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDF2496147.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDISIEKLKEVIAELGFELKGIE"
     gene            complement(42918..44633)
                     /locus_tag="PC407_000672"
     CDS             complement(42918..44633)
                     /locus_tag="PC407_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2496148.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEEARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANINSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEKKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(44633..45187)
                     /locus_tag="PC407_000673"
     CDS             complement(44633..45187)
                     /locus_tag="PC407_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2496149.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(45180..45782)
                     /locus_tag="PC407_000674"
     CDS             complement(45180..45782)
                     /locus_tag="PC407_000674"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDF2496150.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNISIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNSLTHPRIREKIISEIEELR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSKQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(45791..48439)
                     /gene="polA"
                     /locus_tag="PC407_000675"
     CDS             complement(45791..48439)
                     /gene="polA"
                     /locus_tag="PC407_000675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDF2496151.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFKINR
                     LEIDGYEADDIIGTVSKKAENNGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPAQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMVFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDTEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETIPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            48758..48840
                     /locus_tag="PC407_000676"
     tRNA            48758..48840
                     /locus_tag="PC407_000676"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48792..48794,aa:Leu,seq:caa)
BASE COUNT        15332 a         9162 c         5476 g        19392 t
ORIGIN      
        1 aattaaagat aaaaggagta ttttatgaac atacttggat taatagttga atataaccca
       61 tttcataatg gacacatata tcatttatct aaatctatag aaaaaacaaa tgcaacacat
      121 actgtagcaa taatgagtgg aaattttcta caaagaggcg aaccagcttt atttgataaa
      181 tatactagag caaaaattgc tgtcgaaaat ggtgtagact tagttataga attaccaact
      241 ttatttgctt gtcaaagtgc tgaaattttt tctcatggtg ctataacaat tttaaattca
      301 ctaaactgca taaattctat atgttttggt agtgaagaag gaaatacaga aattctttat
      361 aatatagctg aaattttagc aaatgaacca aatgaattta agttactttt aaaaaaatat
      421 ttagatgatg gcctgttatt tccaacagct agagctaatg cactttttga ttatatacat
      481 caagacaaaa aaaataattt tatacaaaat tattcaaagg aacaattatc aaaaacatta
      541 aattcatcaa acaacatact gggtatagaa tacataaaaa acctcatcca tttaaacagt
      601 agtataaaac catttactat ctccagggtc caatcagaat ataattccga agaaattgag
      661 agtgatattt gttctgctac tgctataagg acttcactta aagaaaatac agaccttttt
      721 catttaaaaa atgttattcc agaaaaaact tataacatcc taaatgaaaa agtagataat
      781 ggttttttac ctatgtttga tgacttcttc tttgatattt taaaaacaat tatacttaga
      841 gatatggaca ctttaaaaga gtattttgaa gtaaatgaag gcatagaaaa cagaatctat
      901 aaagatatct ttacatctga atttctacat gagctacaat tagcaattaa atccaagcga
      961 tacactttaa ccaaaatcaa acgaactcta aacaatatat tgttaggtat taaaaaaagc
     1021 gacatgcaac ttataaaaaa catagatact ataccttata taagaatact tgcttttaat
     1081 gataaaggta gagaaattct aaaagagata aaaaataaat ctgaaataaa aataataaac
     1141 aagttttcaa atatatcatt ttcattagat gatacgattt ttaaaacact cataagttat
     1201 gatataaaag ctacaaatat atataattca atatattata aaaacaataa agatttagta
     1261 aaaggcccta tggattttta tactcatcca atatatgtaa agtagttttt taaaataact
     1321 atatttataa aaatcatttt attaactctt tattatataa gatacaatag tcttacttta
     1381 tttatgtaca catgctttta ggatgacaac atcaacttaa atattatgtt actttctaaa
     1441 tttaagtaaa tattgccatc ctaaaaattt tacaatttag cttcagattt aaaagtaaag
     1501 tttaaaatat tatatttaaa tctaatttaa tattttttgt tttgtaaata ttattaaatt
     1561 aatatttcta atatattttc cctggattta aaatattttt agggtcaaaa gctaacttta
     1621 tatttttaat taacatcata taagcatctg aatttgattc atgtaagtac tcttttttag
     1681 catatcctat gccatgttca ccagaaactt gaccactaag ttctctagat tttttataca
     1741 tatagtcaaa agtttctttt aatcttattt tccatgtatt atcatccata ccatctttta
     1801 aaatatatat gtgtagattt ccatctcctg catgaccaaa actttttatt cttattttta
     1861 atttgtcttg aagctcgtga gtgtatctta taaactctgc tatcttatct cttggaacaa
     1921 cgacatcaca ttcatccatt tgtgtcgtag atgcctttat agcttctaaa aatgcacctc
     1981 tggctgacca tatagaatcg tttctttctt gtgtatctga tataaataca tctaatgcac
     2041 cattttctaa acataaatta gctacttttt catattcctt ttctatatct tcagtgctat
     2101 ttccatcaaa tgtaagtaaa agataagcat ctgatgtatt atctggaaac tttttaccta
     2161 aaaattcttc tgctgccaat attacatctc tttccataaa ttctatagcc gttggtattg
     2221 attttgattt tattatttta ggaacagttt ctattgccat tgataaatct ggaaatggta
     2281 taagcaaact tatacttttc tttggtaaag gaagcaattt taatatagct ttagtaacta
     2341 ttcctaaagt accttctgaa cctactaata aatcctttat actatatcca gaactatttt
     2401 taaccacctt tccacctaca tttataattt ctccatttgg tagtacaact tcaagtcctc
     2461 ttacataatc ccttgtaacg ccatatttta ctgctctcat accacctgca ttagtgttta
     2521 tattacctgc aattgtagca cttttttcac ctgggtcagg tgggtaaaat aaatctctat
     2581 cttgaacata ttgacctatt gtcatcaata aaacacctgg ttctactgtc aatgttaaat
     2641 tttcataatc aacttcaagt attttattca ttttacatag attaatcatt attccaccat
     2701 ttatggcaac agcagcacct acaagaccag taccttgacc tcttggtgtg actggtatat
     2761 tatttttata tgcgtatttc attattttag atacttgttc agtttcaagt acatttacta
     2821 aaacttcagg gtacttctca atccctccaa gttcatcatg tgaaaaatct tcatttatat
     2881 cacttccaac taatacattt tcttctccac aagtatctat taaaaaatct atatcttttt
     2941 tatcgattaa tttatacatt tctatccccc tatttagctg ctgatgccat atatttttga
     3001 ttattatctt tgttattttc tattttttca atcaatttag gaataatatc ataaatatca
     3061 cctattaaag ctaaatgtgc tacatcaagt ataggcgctt tatcatcttt atttattgct
     3121 actatatagt cagcaccctg catccctgca acaaattgaa ctgctccaga tacaccacaa
     3181 gttattatca atttaggttt tacagttcta ccactaagac ctatttgttt tttagcatct
     3241 atccaacctg cttctattac aggtcttgtt cctgccactt gagcgtttag cttatcagct
     3301 aatttatata tcatctccat atcttcttgt ttttttattg ctctgcttgc cacaactatc
     3361 acttcagctt cttccagacc aacttcttta tttttttctt ttacactcag taccttaatt
     3421 ttagatttta gttctttttt ttctacttta cataaagtta tctttcctgt agtattctct
     3481 attttttctg gtgctgaaaa tattttatat cttacagtgg caaattgtgg tctattatta
     3541 ggtgtgttta tatgagccat tatatttcca ccaaatgctg gtcttatttg gtctaaatca
     3601 gtatttgatt gaatatcaag tattgtacaa tctgctgtaa gacctgttct aaatctagcg
     3661 gcaagtcttg gagctaatga cctacctaat gtagttccac caactaatac tatcgttggt
     3721 tttattttat ctataaagtt ttctattgct tttgaatatg gttctatcct aaaatcttta
     3781 aattcatctt catcatatac aaatacttca tccaccccat aagaaatcaa attactgcac
     3841 ttatctttta tgtcttttcc tacaaataca caatacacag gttgttttat ttttgatgcc
     3901 atctcacgag ctttacctat aagctcgtac gttacaggat gtatatttcc attatgatgt
     3961 tcaacatata ctgcaattcc cctccattca tctttattaa tttgtacttt actactctct
     4021 ataaattcaa atgcgccttt tggtccactt ttgacacaca acttgcacat cttacagcca
     4081 gcacttattt ctactatacc tgacttttct tctatagccc caaaaggaca tatttctaaa
     4141 attctcttta agtcagttat tttatcttga tttacaacta tctttgacat ttattactcc
     4201 cccttacacc aactttaatt cctctaattt atttacaagt gcaaaactta gttcatctgc
     4261 atttcctctt acaatatttt tatctgtatt tggctttgga ggaaatattc ttacaacttg
     4321 agtaggagaa ccgttaagtc catagttatt ttcatcttta tcttcaaagt catctaagct
     4381 atatacaggt atttctctat ctttagtatt aagttttaat ttaaatgaag gtagtcttgg
     4441 ttgaaaaatg tctttttcta cagttattaa acatggatat tcaatctcac atacttgtaa
     4501 atcatttggc atatctattt caacaactat actttcatct ttaacttcaa ttaattttgt
     4561 aacgttagta acatgaggta tatctaaaaa ttcagctact tcaggtccca cctgtgctgt
     4621 atcaccatca gtagtttgtt ttccacaaat tattaggtct acctttccca tcttcttaat
     4681 accttgtgat atggtatagg aagtagccaa tacatctgct cctccaaatc tcctatcact
     4741 tattaatgct ccttcatcag ctcccatcat aaaactttct tcaattacct tctttgcctg
     4801 aggaggaccc atgctaagta cttttagatt cgctttctta tcttccttta ttctaagagc
     4861 tgtttctaaa gcatataggt catagggatt catttttgaa tctactccat ctctttttag
     4921 tactcctgta gtttcatcaa cttctactga agtcgttcca ggaacttgtt taatacatgt
     4981 caaaatttcc ataaagtaca ctcctttttt atttaactat aactgatact ccatcaggta
     5041 taactactac tttgctctcc ttaccaacta tactattcgc catttctaaa gcttctttaa
     5101 agttaaatgc atgtttcata tgcatttttt ttatcatttc aggattacac ttatctgtta
     5161 ccattatcac agtatattta gataatattc tcgctagtat ctgaaattcc cattggtctg
     5221 gaatcgtatc tactctatcg acacttctaa ttttatctaa aagttgtttt ggagaatctg
     5281 catttgctac attttcataa aaagattgac ctccatgacc atcattacat gctgaaatca
     5341 ttattattac tccaccgtct ttacaagtag cttcagctgc tgtcattcct ttaacagatt
     5401 gataaatatt ttggtctagt ggatatccac catttgtagt tacaacaata tcagatttta
     5461 tagaatttac ttttgataac tctgttacaa atctacatcc tttagtatgt gctttttcga
     5521 catcaccagc aaaggaagca attatttttt tatctttatc tattacaaca ttcaatatga
     5581 atgctaactt tgcaacttta gctgcatata ccatatcctc atgaatagga ttattattca
     5641 atttcccagt tcttgaatta tcactatcta taaagtcact acagtgattg tacattatag
     5701 tctttgcaga tgcgattcct ggaagtacgc tttttctgcc acctgaaaaa cctgcaaaga
     5761 aatgaggttc tataaaacct tctgctatca aaagttctgc ctcatatgct agtttgttta
     5821 tatataaatc tccacctgaa ggcaatatcc ctactttaca catagattta tcatcagtag
     5881 atatatgcat aactatatct tcattatcta ctatttcttc tcccatttta taaattagct
     5941 cttcccttgt acttggccta tgaaatccag ttgccactat aatcttcaca tcaatttctg
     6001 gattaacatt cctaatcctt cttaatataa ttggcatagt tattctactt ggtacaggtc
     6061 ttgtatggtc acttgtaatt atgactacat tctttttccc cttaaccagt tcttccagag
     6121 aattagagcc tataggatta ttcatagatt cttcaactat ctcttcttga gtcttatgtt
     6181 ctatataatt atccgatttt gactccaaaa caccgagaaa acgttcatca ggtatttcta
     6241 aaatcattcc ttgttttgaa tacggtactt ttaactttac catttttaat ctcccattat
     6301 aataaactat aaaattggta tgtggtcata ccagtttcac atttatatga tagtaccatt
     6361 atctaccatt tgcaagaata atttatttta taacaaactt ttaattttca ttttttctat
     6421 aatttttact tataaaatcc atatgttcct ttgctttctc acatgcaagt tctacatcat
     6481 tttctacaac tgcattgtat atagcttcat gctgtttaag caatatattt tcattaaaag
     6541 gttctgcgat tattttttct cttgcatctt ctataaatcc atcaaataat tttgatgctg
     6601 tcataaaaag actttgaatt aaataatttt tacttaatgt tgctagttta ctgtgaaatt
     6661 tttggtctaa taacaccagt actcgattat tatctttttt attagtttct actctcattt
     6721 catctattat atgtttcaat tctataactt cagaagaagt tattctcttt gaagcaagtt
     6781 ttgcaatctc aatttctagg attattctaa gctctaatat atcttcaagt gtgccattat
     6841 taagtttaaa aatcatagat aaaggttcta ttaaagtttt ttctatattt gtacatataa
     6901 aatttccttc accctgtctg ctttctatca ctcccatagt ctctaatacc ctaatagctt
     6961 cccttatgga agttctactc acattcattt gttcagacaa ttctctttct gatagtaatt
     7021 tatccccctt ttttaactct ccatttagta ttttatattg tatttgctca attacttgtt
     7081 cataaacttt tttactagct atactattaa acattttttc ctccaatatg ataatcgttg
     7141 ttgtaatata tatctattat atatcttaaa ccattacatt catatatttt tattactttt
     7201 aatataatat cttataactc aaatttacag gtgatattat gaaaaaaaat aaaattattg
     7261 ctttttttgc tccagctatt atagttggag ttctagtttt agggattatc ctatacccaa
     7321 atgaagctat aaaatctgca agagaaggct tctctatatg gtctaatgtt ttagttccat
     7381 cactactacc atttataatt ggagcaaact taattgttga tttaaaaata gtggatatta
     7441 tagggtttat catcaatccc ataactagat ttgtatttaa tgtttcagga aagagcgctt
     7501 tagcttttgc tatatctaca gtatctggtt atccagtagg agtaagttta gcttctgaat
     7561 ttaggagtaa tgggcaaatt tctaagcatg aagcacaaag acttgtgtct ttttgttcta
     7621 catcaggacc tttattcata ataggttcgg ttggtacagg tatgttccaa aattcaaact
     7681 taggttattt aatgattctc tgccattatc taggaactat attggttggt cttttcttta
     7741 gaaattatgg taatgaaaat cttccaaaga caaaattgga tttaaaaaca aatataaaaa
     7801 atgtaatcaa cattagaaat tcaagtggga atggtttttt tgttttattt ggaaatgctg
     7861 tatttaatgg agtaaataca ttacttacag ttggtggatt tgtaattgtt ttttcagtag
     7921 tatttaaaat actatctctt tttaatgtaa tctcattaat agcatcagtt atatatttgc
     7981 ctctttcatt tttaggtgtt agtaaagaac tctgtcaagc atttgtaagt ggtctatttg
     8041 aaattactat aggatgcaat aaagtatcta gtataacaag ttctccagaa attttaagag
     8101 catctttagc tagctttttg ataggattta gtggattatc catcctagct caatgttgta
     8161 catttctatc aaaaacggac atagatttaa aattatacat actaagtaaa tttgcgcatg
     8221 gagttttagc tgctatcttt atatttatac tttatccaat tgcaaactct gctgtgttgg
     8281 tttctagctt tgctgataca tacaacgtta tatataataa tttaatctgg ttttattatc
     8341 ttagctatta tgatacaatt cttcaaattg tagtaataat ttatctaatc tcagcagtat
     8401 ttattgcaaa aaaacatatg aaaactttat ctacaaatgg gaaactaatg cgatataaaa
     8461 atactttttt tattaagaaa ttttcacgaa aaaaataaaa ctaataaaac ttaactaaat
     8521 acttccattt ttttattata tttgtcataa ttttaataac ctttttaatt agtttaacag
     8581 catagttatt tcatcacaat ttaataattt tattagaaat atttttcaaa aaattctcct
     8641 taatcacata tatcacactt gtataagttt actgggaaat ttttccatta tataatgttt
     8701 catttatatt atataataaa taccgaccta aacttaccaa atcaaactaa gaggagtggt
     8761 tgaaaatggc aacaagacct atagaaatag acgaatataa aaagataatg gaactattac
     8821 atactggctt tacttatagt gaaaatggcg ttgaaaaaag atttagaaaa aatcctaaag
     8881 ttgcattggc actaatgtta gaagctaatt taggtctaag aatatcagat attttaagat
     8941 taaagatagg taattttaaa ggtaatatgt tggaaataaa tgaaaaaaag acgggcaaat
     9001 tacagtatag accagttaat aaaaatataa ttgagtccat aaatgaacat gcaagaaagt
     9061 ataatcttaa atcaaatgac tacttagtaa atataaagac aaaggctatt caaaaacagt
     9121 tgaggataat ttgtaaacac ttaaatctgt ataatataag tacacatagc tttagaaagc
     9181 tctatgctac tactcaattt gaaaagagta acaataattt agagttggtt aaagaacttt
     9241 taaatcacag ttctgtagcc actacacagc gatatataag agtaactcag caagctatta
     9301 atgaagctag tgaaaatttc tttattggtt aattataacg tatacatttt tctattatct
     9361 tattaattca ccagatatta aaaaagagtg tctcaaaatg aactttttag ttcatgagac
     9421 actctttttt tataaaatca aatatatttt tattatttca atactggaaa aaaggcttgt
     9481 ctctctattt tgagacaccc tattaaattt tcataattct tgcataaaca tattaatcta
     9541 tcttcaataa ttttttagct gattcactgt aattatttct ttttacaatt tccaaaactc
     9601 tatcaatctc atgttctgac tttgcactta caaaaatcgc caattcttca tcatctataa
     9661 tatcataact atctacatat ttatctaaac attctaaaat tccatcttta ttattatacc
     9721 caccaaatct aactactgca ctacactcat cttctataag tccacttaaa tccaatttcc
     9781 cttttgaaaa agactttata tttgaacttc tattatctat tatatctata atatctgcat
     9841 aaacagcact tcctgttgca cctcttccag ctcccttacc tacaaaagaa agttcaccta
     9901 ctgaatcacc tactaaaata actacgttga actcattatc tatctttgaa agcatattat
     9961 tattgtccac taatgctggt ctaacaaatc cacttacttc tcctgattca tacttacttc
    10021 tagcaaccaa ttttatctta caatttaatt tttttgcata ttctatatct ttttcatcta
    10081 ttgaagttat tccttctaat aataaatctt tccaatatac tatttttcca taagataaag
    10141 tagacaatat agataacttt ctagcactat catatcctaa aacatctgca tctggattaa
    10201 cttctgcaaa tcccaactct tgtgcctgtt ttaaagcaac atcatatggt aaattttcat
    10261 catacatctt tgataatata aaattacaag tcccatttaa aatagcgttt atgctcttta
    10321 tattgttgcc ttccagtgat tctatcaatg gttttaaaac tggaatacca cctgccacag
    10381 atgcttcaaa cttgatgcta acattattta agttagctaa ttctataagg tcactacctt
    10441 tttcagccag taaatcttta ttagctgtca ctacatgtat ttttttattt aacgctcttg
    10501 ttacataatc aaaagctgga tttaatcctc caattacttc aactagtata tcaatatcct
    10561 tgtcaaatat atcttctata tcagtagtta tcttatcaaa atacttctta tacttgtgtt
    10621 tttctttatt tttaactaaa attccaacaa cttctatatc tctttttgaa gtattatttt
    10681 ctattaaatc aattaatcca cttcctactg ttccataccc taatattgct atcttcactg
    10741 taacataccc ccatcatttt gattatatta aattatttat atatataaat ataccaataa
    10801 aaaaagcttg agtataaact caagccatat gacgggttta tactcatctt tcagcataaa
    10861 tgctgcagga tttagcacca atgtataatt aaatatactg gttgccgggc ttcacagggc
    10921 cagtccctct gccactcttg ataagattgc taattaaaat ttatgtttaa tagtataata
    10981 caatttcttc caattgtcac tattttttta acttatttcc ctaaaaggat ataaactatt
    11041 atctaacagc tcttttagtt ggtttaccct tttttgtaac attattattt ttagtctttg
    11101 ctttcgcctg ttcatgtcct ggaaaaagta ttttaggctc ttcaatattc ctcttgtaaa
    11161 tagtaaattt aaaaccaata gcctgcacaa attcagctct taaagcttca catactgcat
    11221 tagcagtttc tttagtatca agacctgcat tttctaatat agtaactttt actaattctc
    11281 tagttcttaa cataccatct aattgctcta agaaactctc tgtaacacct tctttaccaa
    11341 tttgtgtagt aggctttaaa gtatttgcta aagatcttaa atacgccctt tgttttcctt
    11401 ttaacatatt ctcacctcta taattctata cttaaattat ctataatcta tatttaaatt
    11461 aattgtaaaa ctcaaattct aattcataaa ttttaaccac atctccatct tctattccca
    11521 tttctctaag cctatcaaaa acaccttgac tctccatagc tttttggaaa tattgaaggg
    11581 attccatatc atcaaaattt acagaataca ttattcttct aagtgcttta cctgtaacta
    11641 catacacacc atcttcaata tctatactta actcttcttc agtaccaata tcaagttctg
    11701 gtctatacat ttcttcttct gaaactagct ccacatcttc tacttctttt agcattttag
    11761 aaacataggc tattacttca tctatgcctt gacgagttgc tgctgacatt ttaaatactg
    11821 tatatcctct gccttctaat tcactcttaa acttctcaaa cttactctca tcttctaata
    11881 tatcaaattt atttgcaact acaacttgtg gcctctttga aagcttttca ttgtacaatt
    11941 taagctcatc atttatcttg tcaaaatctt caatagggtc tcttccctct aatccagata
    12001 tatctacaat atgtatcaat acctttgttc tctcaacatg tcttaagaac tcatgaccta
    12061 atcctatgcc ttcagaagca ccctctataa ttccaggtat atctgcaagt acaaaactat
    12121 ctccaaactt agtttgaaca acacctagat ttggtgtaag tgttgtaaaa tgatagtttg
    12181 caattttggg tttagcttta gttacaactg atagaaatgt tgatttacct acatttggaa
    12241 atcctaaaag accaacatct gctatcattt taagttcaag agttatccat ctttcttctc
    12301 catctgtacc agattttgca aaagctggag cttgtcttac tgcatttgca aagtgttggt
    12361 tacctttacc acctatacca ccttttgcaa ctatagctgt atctccttct ttttttaaat
    12421 ctgctaatac aagacctgta gcctcatctc ttattactgt acctgctggt acttttaata
    12481 ctaaattttc tccattttta ccagctcttt ttcctttgct tccgtcgcca ccattttgag
    12541 cttgatattt tttctggtac ttaaagtcca ttagagttct aagccccaaa tcaacttcaa
    12601 atatgatgct tgctccacga ccaccatctc caccgtctgg accaccagct ggaacgtatt
    12661 tttctcttct aaatgctacc gaaccatttc caccatttcc agccttaaca aatattcttg
    12721 ccttatctat aaacaaattc ttcacctact taattaaaaa cttttaatta gcattaccaa
    12781 aaataccatt ataaattcat cagtattata agaaaaagga gtgcctcaac actcctttta
    12841 tgtatattac tcagctattg ctactggata tatactaact ttttttctct tcttgtcttt
    12901 tctttcgaat ttaacagtac catcaactaa tgcaaataaa gtatcatcac taccttttcc
    12961 aacattagtt cctggatgta ttttagttcc tctttgtcta actattatac ttccagcagt
    13021 tactaattga ccatcgaatc tcttaacacc taatctttta gatatagaat ctctaccatt
    13081 tttagaagaa cctacccctt ttttagatgc taataattgt aaattcatgt ttaacatatg
    13141 gagtcacccc ctactttttt aaaattttta tattttttgg gtattcccgt ttaatttctt
    13201 taaccgctaa ttgaaagtga tttagtaata attgtgcgtc ttttacatgc gtatctttga
    13261 ctatacactc tatatgacct tcttctccta ctactttttc aaattcaatc ttttgtaatt
    13321 tgtctaaaga atttataact gcaatagcat tagatgtaac tgcagcacat actatatcat
    13381 agcctatttc agcaaaatca gcatgtccct ttaaacaaaa accttgcaac aagaaatcat
    13441 cattataata atatttaact tttatcataa cttaaccaat aactaagcgt ttattttttc
    13501 tacaactatc ttagtgtatg attgacgatg tccttgtttt cttctgtaat ctttttttgc
    13561 tttatacttg aaaactataa ctttcttagc tttaccttgt tcaacaactt tagcttgaac
    13621 agatgctcct tctactactg gagaacctaa ttttaattct ccatctttag agcaagctaa
    13681 tacttcgttt aaagtaacaa catcacctgc gttagcttct aatttttcaa cgaataatac
    13741 atcaccttca gaaactttat attgctttcc accagtcttt actatagcgt acattaaata
    13801 cacctcctcg gcctcaaact cgccagtaca aggtacttca tttgaagatt taaaccttta
    13861 ctgtgcggca ttacaattat taattataaa atatttacat tttattgtca actattttat
    13921 cgaaaataac attggtttta ttgtgcttta atgtactatt tgcgtttaaa gaaatcttta
    13981 tattatattt ttcaccaatt ttatcaatta tatccatata atattctttt atttgttcaa
    14041 aaataaaatc attaaattct accattatgt ttttgtaaac agtatgttca cttattctca
    14101 taatttcttt ttcaatacta tctagtatat aatgtacaga ttttattgtt tgttcaccat
    14161 cacaacatgg acaagacatt aaataatact tatcaatgga ttctttttct ctacgcctag
    14221 caacttctac taaaccaagt tttgtcatac ctaatacttc agcttttctt ttatctttat
    14281 taaattcttc ttctaggatg tgtaaaatct ccttttgatg cttcttttta tgcatatcaa
    14341 taaaatcgat tattattatc ccagctatgt ctctaattat aagctgtctt acaatttctt
    14401 ttgcagcttc aagattagtt ttataaacgg tttcatcaag tttaccagtt ccaataaact
    14461 tacccgtatt tacatctata accgtaagag cttctgtttt ttcaattatt aaataacctc
    14521 cactctttaa ccatactttt ttatttaata atttttctat tttagattct attctgtata
    14581 aatcaaatac atctctattt tcctcaagac acagtttatc aacataagtc ttatctatac
    14641 ctcttaaaat agacttaagt tcagagtact tatcataact attggtaatt atagattcaa
    14701 tatcatcatt cacattatct tttacatatt tagttgcaaa atctaaagac ttatataata
    14761 atttcggacc tataccaagt ttatattctt ttaaaatatt ttcatacttt aatttcaact
    14821 cttctatatc tttctttatt tcatcatgag tgcaaccttg agcctcagtc cttattatta
    14881 atcctagatt ttccttattt actgccttag taattttttt taatctaaat cgttcttttt
    14941 catcagtaat tttatttgat attgtagttc tatcattgga aggtatatag acaatatacc
    15001 ttccagaaat actaatctca gtgtttaatt ttgcgccttt agttcctatt tcttctttat
    15061 ttatctgaac caaaatatcc tgaccagatt ttatagtact tccctttttt aattgtaaat
    15121 aagcaagttt cttaaatcca atatctacaa aacaagcttc tattcctggt ataacttttt
    15181 ttacgattcc acgatatata ttggatacag tcttcttatt taaattgtct tcaacaaaaa
    15241 gttctgttaa tctttcatct tcaagtactg ctgttttctg ggaccctatt agggactcaa
    15301 taactatctt tttcacaact tcacctctcg agcttacata ggagtcacta attccccatc
    15361 ttctaataca tataaatctc ttctcaatat atcaacatct aatgggtcaa tatcaagttc
    15421 aaacatctct aatatctttg gtataagtat attagtattt aaattagttt ttgagcctgt
    15481 agctatagta gcagtaagag taatatgttc atcttctaaa tttaaaacat caaaggttct
    15541 tatcataggt cttatatcta cttcaaccat cttacctttt ttattctttt ttgttatgat
    15601 tatttcttta ttattcataa agtcaattac ttttcttttt acaaactctt tcgtcatagg
    15661 tcttttcaaa tctatattaa acaaatattc tccatagtct atcacagaag aaagagaagg
    15721 tgtttgtcta tcaatttctt ttgctttgat aaaatctatc ccatctggaa gttgaattga
    15781 tactttattt aaaaattcct ctactgataa atcatcttct tctatttcaa tatctacata
    15841 ctcaccttga ctttctgtcc ctaaagccaa agcattgcca taactcattt tagggtgagg
    15901 attaaatcct tgagaatgtg ataagtttat ttcagctctt ctaaacgctc tttgtaacaa
    15961 tctctgaagg tcaagatgtg atatatatat catgtcgcct tcctttttaa attttactct
    16021 tattatctta ctcatattag caaagccccc ttcctatatc atgtatattt attccacaac
    16081 cattgcagtt atgtctacaa tcagaagtga ttttttcttt tttagcattt tcgtcttctc
    16141 taattaagaa ttttttacta atacctacat ctatatggtc ccaaggaaat acctcttcat
    16201 agtttctatt tctatgtgca taaaaatcta tagaaagatt attcttttcc atagcttctt
    16261 tccatatatc taaattaaaa tgttctgccc atccatcaaa tttagcacct agtctaaatg
    16321 catcatatat aacttttcct attcttctat cgcctctagc tactactgct tccatcaaac
    16381 tagtctttga atcatgataa ttgtatttta tattgttatt tcttaattta tttactaaat
    16441 gtctctgctt attaataact tcttctgtag tatcttgacc atgccattgg aaaggtgtaa
    16501 atggctttgg tacaaatgta gaagtactca ctgtaacact aaaacttctc tttagctttc
    16561 ctccatttac ttttctataa atatcaataa ctttataagc taatttagct ataccatcta
    16621 aatcttcata agtctcagtt ggtaaaccaa tcataaaata caacttaaca ctattccacc
    16681 ccatctcaaa agctctttct gttgcatttt ccaaatcttc ttctgtaaca cctttattta
    16741 taacatctct aagtctttga gttcctgctt ctggagcaaa agtaagtcca gattttctta
    16801 cttgttgtat tttatcagca atttccattg aaaaattatc taatctaagg gatggaagcg
    16861 aaatcccaat attattagat gcatattcat ctaccaaata atcagtcaac tctgataact
    16921 tactataatc actcgtacta agtgatgata atgatatttc atcatatcca gtattcttaa
    16981 ctaatttatc taatatttct ttaagtcttt taacactctt ttctcttatt ggcctgtata
    17041 tcatacctgc ttgacagaat ctacatcctc tagtacatcc tctaaataat tctagtacta
    17101 ttctatcatg aacagtatct atataaggta ctattagttt ttctggataa tctacagtct
    17161 caacatcttt tattatcctt ttagtaggat ttttagtaat cccttctcta ttaggagtta
    17221 cactttgaac tgtaccatct tcattgtact tgacatcata aaaacttggt atgtatactc
    17281 cttctatgct agatatttta tataaaaaat cttctctagt cgttttattt ttcttccatt
    17341 ctttatactc atttactact tctaaattaa cctcttcacc ttcacctaat attactacat
    17401 ctataaaatc tgctaatggt tcggaattat aagcacaagg accaccaacc attataaatg
    17461 ggtcttccaa agttctttct tcttttaata ttggtatatt ggctaaatct aatatattta
    17521 agatattagt gtatgaaagt tcatattgaa gtgtaaaagt gacaaaatca aagtttgtta
    17581 taggctctct tgactctaat gcaaataatg gtatactttt ttctctcatt atattttcca
    17641 tatcaactgc aggagaatac actctctcac aaaatacatc ttcatcctta tttattacat
    17701 catataatat atgactccct aagtgactca taccaacttc atataaatct ggaaagcaat
    17761 gtgcatatct tatcaattca ctattactag tatctttatg aattgaattt atttcatttc
    17821 caagatatct agcaggcttt tctacttttt ttaagatttt tttcaagtct actttattca
    17881 tttattaaaa ttcctccaaa caagctcttt atttttattc ttatttataa gtagtattat
    17941 acacgataaa atattttcta tcaaaaatat aactaaaata ttgaatttaa aatacatatt
    18001 attcacaaaa tagaatagct aaaaatattt aagaatattt tctattaaat ttttattgaa
    18061 atacaatttt tttacaaagt atgatatatt ataaacataa aactattgtt ttacatgact
    18121 tatcatgaca tcttttgttt cattcacctc aaaattgggt atgttactta tataatgata
    18181 aattttagga gggttaaaaa tgagtaaagt ttttgaagta aaaaaagata tctactttac
    18241 tggcgtagta gatgaaggtt taaaagtatt cgacataata atggaaactg agtttgggac
    18301 aacttacaac tcttatttaa tcaaggacga aaaaacggtt ttatttgaca ctgtaaaggc
    18361 aaattttaaa gatgagtttt taagtaattt atcagaggtt actgatatag ctaaaattga
    18421 ctatgttgta attcatcaca cagaaccaga ccatgcaggt agtttaaaat acttattaga
    18481 tataaatcct aatatagaag tttattgtac taaagctgct aaactatact tagatggtca
    18541 aataaataga ccttttaact gtcatgtaat aaaagatggt gaaattttaa atatcggtaa
    18601 aagaaatctt agatttataa ctgctccatt cttacattgg gttgatacaa tgtttactta
    18661 cattgaggag gacaagactc ttttaacttg tgatgcattt ggttgtcact ttgcaagtgt
    18721 tgatgcagag gtagtgaata gtgaagatta tctaaaatca gctaaacact actatgattg
    18781 tatagttaag ccatttgcta aacatgtttt aagtgctgta gataaggtgg taggactaaa
    18841 catagaattt gatactatat tgacttctca tggtccaatg cttactaaag acccaatggc
    18901 tgctgttaaa agatatgttg agtggtctac agaagctgtt aatacaacta atcaaaatca
    18961 agtttctata ttctacttat cagcttatag taatacttta gagatggcta aaaagataaa
    19021 agaaggactt gataaagaag gtgcaaaggc tgaattatat gatttagaag atatgacatt
    19081 aacagaaatg cacgatacat tagtagtgtc aaaagtaata ttactaggtt ctccaactat
    19141 aaacaaaact atggttaaac ctatgtggga tttattctct gttatagacc caatggctaa
    19201 ccaaggcaag atagcaggag tatttggttc atttggttgg agtggtgaag gtataactat
    19261 ggctgagact ttacttaaat ctatgtcatt caaaatgcct gtagaatctc ttaagaaaaa
    19321 attcttccct agtgaagaga cattaaaaga gtgtatggca tttggtgcag aatttgcaaa
    19381 attagttaaa tagccattaa tcacacataa tattatttaa aataaatgat tttatattta
    19441 aaaataaatt aaaatggagt tgtctcaaaa ttaatttttc taatctaatt ttaagttcaa
    19501 ctcctttttt atttcccaaa atgactaata taatgataaa tgcaccctca ccaagagggt
    19561 acattattta taatcttttc tatttgtata cttgtatatt tatattaatt cacatatcta
    19621 gcatatctta cttgccctct tccagaatta ggttctattt caccaatttc tattttaata
    19681 attacattat tgtttttaca cttttttgat aattctgctg cagaaaggtt tttaccagta
    19741 aaatctaaag aaaatggctt atcatattca aaaggatact caacaggtac accatctgga
    19801 gctaatgcaa ccataccata tggtgtattc ataaacccta ttacagaaac tgtatgcctt
    19861 gatgggtcta aatttgaatt tttgcttgta aacttaattg tatttattga tgagttgatt
    19921 aaactacacg gaactccctc atacctttca attttgggac gtagattatt tccataaact
    19981 tcatcaagta ttgcttcttc ttgatctgct atcatagtgt gattcccagg tccaactggc
    20041 gcagaaggct tagctggcgt aggtttctta acagttggcg ttggattctg tgtctttact
    20101 gattcaccaa cagctggata ggctttcttg tcaaatggta atgggtgagt aagactcttg
    20161 tatatgatgt ctgctcctgc tgatttgttg gcatcaatat ctacatgata caaacgtcca
    20221 tcctcaaaac gaataagatt ccactgatgg tcatttgttg tactatgtac ataataacat
    20281 tcaattccca tagcatcaca taaagctttc aagctcctac acattccaga acaagcacct
    20341 tttttagtaa ccaatgtata agcagtattg gctccctctg ctgcgttcca gttaacatat
    20401 gttgtgttgt ttttaatgtg attataagca accctaatct tagcatctgt ggtcatgttc
    20461 tttgtaattt tcttgttcat aaagtctgct gttgcttgtg ctaccgaatt aagttgagct
    20521 tgtgataact ttggaccata tatactgttg gttccagcta catagctttt cggtgtcttt
    20581 gcagtaacat ctttagctgt cataccataa actgttacac tcatacatga ggttaaaacc
    20641 atcgttgcaa tcaatagttt agtagctaat ttcctgattt ttttcatatt agcattctcc
    20701 tttataatat attttacttt caattcccta ccattaatta agttcatttt agatttaaaa
    20761 tatggattat caacatgtgt gcatagcaat tcacgtggtc atagaatatt tagatttctt
    20821 ggaaaatctg ttctatattt cctatatagt tcgtacacaa tccaactcac aaacctgcat
    20881 ttttgatatc atcacaataa aatgataata attaatatat tcaaaaatgt tggtctgtaa
    20941 caggtttgtc aattttctaa ttattggtaa tgacgagagt gaggagaatt gtcaataata
    21001 catttaaaat taaaattgta tacacttcat acattctccc ctaacaacat tatacagatt
    21061 ataatttgta tattcaatac attttttgga aggtataatt atatcaaaat atgtgattat
    21121 gtatagatac aatccactat ggagaaatat aaaagaaaag agtataacaa acaataaaaa
    21181 ataagatgat tcaaatttta ttttgaaaca tcttatttac tctacaaatt aaaattattt
    21241 ggtgaattat ttaattatta ttttttatta aagaaatttt aaatcttaat ataaaatacg
    21301 ctattataca taataatcca aatgcaaatg ctactggaat taacatctcc gattcaaaat
    21361 aaaagaaata ttctaagaaa atatctggat ttactatatt cccattcttg tctaaaaaat
    21421 cttttccatt atatcctaaa tgctgtataa aattacttat gttattataa tctgcttccc
    21481 tctcatatac catattagca ttcaatccag aagaacttaa attagtgttt ccatctttca
    21541 tatctattac atatagtcta agagctttga ctttactata cttatcttta tcaagtattt
    21601 tttcaagttc taacttatat tcaccaatat ttgtcatatc atgtctttct gccaattgct
    21661 ttatttctcc atcaagttct tttcctaaat tatacatatt atgttgtaaa ctattattgt
    21721 aataccacat aaatccagaa attaaaaatg caattatact tatgtaacct aatacttttt
    21781 taaaccccat tacaattgat ttatgcctat ccaatgataa agatagttct tttattatat
    21841 tacataattc atactgcatt tcatcaccat catcaaatct ttcaattgct tttttacatg
    21901 cttcttcttc atctaatcct tctaatttaa attcatttgc agattctata agatggcatc
    21961 tcatttcctg ttttaattct atagtacttt tattgtcaca ttttttatat agtttatcta
    22021 aatattcatc tattagtttc ataaattaat ccacctctcc caaaaattga ttcataacct
    22081 ttttaataaa tctccattct tgtttcttta tttctaaaaa atctttacct tcattagtta
    22141 aattataata ttttcttctt ccacctgaac tttgttcatt gctataataa gatttaatta
    22201 aatttttaga ttccattctt ttaagtgcaa gatacattgt tccttctttt aattcaaact
    22261 cacttctttc tcttacttgc tttgcaattt catatccata acaatctttc ttttctaata
    22321 tagaaacaat taatatatcg atataacctt ttaacacttc tttatttaac tgcattatgt
    22381 accaccgcct tttcacctac tactctataa tgtatagtac ttatatttac tataataata
    22441 catagtactc tataatacaa ggtttatatt ctacaataga cttaaaaata ataaatagct
    22501 gtatattagt atttatagat taaatactaa tatacagcta tttatcaaat tttaaaaata
    22561 aatttttgtt ttattaatgt tgtcgtaaca acatttactt ggttgaaata gtattaccta
    22621 taagtttaaa tatatataat aatagactta attctatttc atattattaa aatagagtaa
    22681 tagtttaaat tctactactc tatctataat ctatttccta attatttaat ctcaaattta
    22741 gctcctcatc atttaaatct ttcataagat ttctacaggt actaggttta ttgcaacaga
    22801 aaaactaggg cattaaacac ttatatatct gtttctttag actgtaatat taatattttt
    22861 taaatacgtt gacttttgct ttttctaact tctctccatg atagttctcc tctatcaaga
    22921 gcttttataa atatttctgc tgaagctaga tttgtagcta ttggaacatg ataaacatca
    22981 caaagtctta ttaacgcttg tatatctgtt tcatgagcct gagatgtcaa tgggtctctt
    23041 aaaaaaataa ctaaatctat ttcttgtgtt actattaaag aacctatttg ttggtctcca
    23101 cctaaaggac cagatgctaa tctatttata tttaattcag tttcatccat tattcttttt
    23161 cctgtagttc cagttgcata tagtccatac ttttttaaaa ttgattcata acctatacaa
    23221 aatccgacca tagtgttttt catttggtca tgtgctacta atgctatatt catatatcta
    23281 atctcctcaa tttatgttta gatgtattta aatttagaaa tttattttct ttcttctcat
    23341 acacacattt agtactaatc ctaagtttgc taaagatgtc aataacgaac ttcctccata
    23401 acttacaaaa ggtaatgtta ctccagttac tggtattaac gcaaccgtca tacctatatt
    23461 ttggattatc tggtaaccaa acattcccat aacaccaacc actatgaggg ttccataaaa
    23521 atcttttgca tctcttgcaa ttgctaaaag ccttaatagg aatatcataa ataagattat
    23581 caacactgcc attcctatta caccaagttc ttcacctaca acagcaaata taaaatcact
    23641 atcttgtaca ggtaaaaaat cttcttgatt ttgacttcca ttgtaaagtc ctttaccagt
    23701 tacgccccca gaaccaattg caatcagaga ttgcataact tgatagtttc cttttagagt
    23761 tacatcttct ggatttagaa aagcttctat tcttaccttt tgatggtctg ccatcattaa
    23821 atacatcagt ggcattgaca ctaatagtat gattattcct ctttttatca actttgagct
    23881 aagacctgct gtgaacagca ttgcaaatat catacaacaa aatacaattc ctgttcccaa
    23941 gtctggttgt gcaatcaata atccgataaa tggaagagaa taaactacaa caggcataac
    24001 ttcctttaac gtatttaatt tatccttttt ggattcaagt atctttgcat aacttagtac
    24061 aaatgttaat ttgactattt cagatgtctg caagtcaagt ggtcctaagt taatccacga
    24121 cctagctcca ccttttactg tcccaattcc aggtaataaa actatggcta acaaaataag
    24181 acttattata taaagcgctt tataatacct gcctagaaga ttatagtcaa aaaataatat
    24241 gactataatc attcctatac caagtacaaa agctaatcct tgtttaatca attgattata
    24301 tgaacctgtt gaattagcat gtgttgcact actaagtata acaagcccaa aaataaagat
    24361 agctaatact gtaactataa gtttccagtc taactgtttt atcaacttaa ttgtcgactt
    24421 ataattaata ttgatttttt tcaaagaatc accgtctttc tatctttaaa catataatta
    24481 aataaaaggg gcatatacca ataaggatat ccccctttta agcactaatt acatacatct
    24541 atcttttata ttctttagtg gtatattagc aactaatgca gatacaggtt tgtcatcatg
    24601 ctcacctctg catttagaca tttttatttc aagaccatca tcttctattt ctgcataatt
    24661 agctattact tttaaaatgt ctgtttttat catctctaaa agttgtggtg aacaatctac
    24721 tctatcatga actaaaacta gctttaacct ctctttagca acagatttac tagttttagc
    24781 ctcgttagaa aaaactctaa ataaatctaa cacagatgta tcctcctttg ctacttagcc
    24841 atgctaaaca tttttttaag tctaccaaag aatccatctt caacttcaag atcaagtaga
    24901 ggaacttctt catttagtat cctttttgcg atatttttgt atgcttgacc agcaagtgac
    24961 ttagaatcaa gtatagctgg ctctccttta tttgttgata taattatgct ttcatcatca
    25021 ggaacaagac ctaacaaatc tattgctaat atttctatta tatcttgttt atccatcatg
    25081 tctccacgct taaccatatc atttctaatt ctatttataa ctaatcttat ctcttttatt
    25141 tcatttgctt ctaatagacc tattattcta tcagcatctc ttactgctga tatttctgga
    25201 ttagtaacta caatagctct atctgcccca gctactgcat ttttaaagcc ttgctcaata
    25261 cctgctggac agtctattat tatatactcg aaagattctt ttagtttctc acacagatca
    25321 atcatctgct caactgagac agcattttta tctctagttt gtgctgctgg taataaatat
    25381 agattatcaa atcttttgtc ttttattaga gcttgcttaa gcctacaagt tccttcaact
    25441 acatctacta tgtcataaac tattcgattt tcaagaccca ttactacatc taagtttcta
    25501 agtccaatat ctgcatccac aactactgtt tttttatttt ctaggcttag cgcagttcct
    25561 aagtttgctg cggtagtagt ttttccaacc ccacctttac cagatgttat aactataact
    25621 tcgctcataa tactaccccc tatttttctt tctatttgtc taactttgac aaatagcttt
    25681 cgattacaat tcttccatta cttacaaagg ctatttctgg gctaatttct gattcatttt
    25741 cataattttc ttcatcatct ggagcttctg caatattatc tgctatttgt aatattttag
    25801 gattaagatt tccagcaaca acatatgcaa attcattgcc ttctgcacct gcatgtacaa
    25861 aaccttctat tttacccatt acaaaaacat tacctcctgc tacaacctta gcaccagcat
    25921 ttatatctcc cataacaact gtatttgatt ttgaaaggac ttttgaacca ggtttcatat
    25981 cattcaatat aactacatca ccttcaaatt caatattttc accagatctt agggtattga
    26041 catattttgt tggaaaatta ctattttctt caattttttg atcttcaaca aattccacat
    26101 caaatctaga agtaattcct tctttaagtt ctagaatctc catgtcagtt aaacaatcac
    26161 tattaatttt actaatttta gccccattaa aaaagccaac atatgcttcc aatttgttta
    26221 ttattttttc ctgtatttcc tcaaatggag cttctctttt tatatttact attatccctc
    26281 ttttattacc cttgaactct actagttctt gactacaaat ttctcttaaa gacataacaa
    26341 cctccagagt gatatatcat ttatattctt caaatacgct ttaaaccctt ttattttttc
    26401 tatataaagc taaagtttat acatagatta agctatctat taatttgtga ctcgaaattt
    26461 atattttcgt cagaagaatt gttaacatca ttcgtcttgt tgtcattttt actattagtt
    26521 ggctctaatc ccatataagt tccaataact tctctagttg gtaaaagtga gaatacactt
    26581 gaatcacctt gaggaattac acatacaaca gctatttctg ggtcatctgc tggagcaaaa
    26641 gcaacagtcc atgtaaaaga accataatct tgtttaaatc tatctatttg gtcatctgtt
    26701 atttttggat ttaattcttt tatagctttt cttagatatg aagaattcac cttatcagtg
    26761 tcctctaatc taacttttac tccatcttct aattcttcct ctaatttctt tctttcttcg
    26821 tctttcaaat ctttattttc taatttcttt tttatttcct tttccttagc taatgaaagc
    26881 tctttttctt tttctgcttt catcttatca gctagtttaa tcgcatcatt taggttgaca
    26941 ttataagatg acatatgact ttttaaatat tcatactcgt tatctgtagg tattttccca
    27001 cttttctcag ctgtaccagt ttttgctgct acatctattg gaaaatcagc aaaagcactc
    27061 ttcgctgtac cttgtcttgc aaccaatttc ataccttttg ttaattcttt taagttatct
    27121 ggatttttaa aaggtatttt ttcgactttc ttctggtcat taattttaac tgatgaataa
    27181 tcacttgaaa cagctctatc aactacagat aattctacta aattacctcc atttgctata
    27241 gcagctacat atcttgatat ttgagctggt gtataagcat tttcaccttg acctatagct
    27301 aagttaaatg tatctgcagt actccatttt gcaaagttaa aataactgaa tactgctaaa
    27361 tctgctactt cttctattct atcttcttta acattcattt tctttagcct attcattgtt
    27421 tcgactctac caggagtttt cttttctgct gcccagctta caatctcatt gattcttttc
    27481 tcatattctt taggattttt ttctctagtt atatcaacaa aatcatttgc catttctctt
    27541 tctaaatttg actttagaag agcttgtgtt gatttcaatt tatcttctgt gctaggaact
    27601 ttacctgctc tttcttctac ttcatcttga agccctgtat tttggtcaag cccaaataat
    27661 tttgcatact caagtatttt atttgcattc atatctatac caatgctttt tccatctggc
    27721 catgttttac ctgtaccaat tgttgccata tatatattac aagactcttg tatcgcttta
    27781 tataagtttg tcataccatg gtttcctcta ccatggttcc atacatagtc accaaaagat
    27841 tttttaccta acattataac cccagggtca tttatagtaa agtttggatc caaaccattt
    27901 tctaatgctg ccattgaagt tagcatctta aatgaagaac ctggctgaaa ttccccttgt
    27961 gtaaccaagt ttaaaagtgg acttcctgct agtaagtcat ttggattttt tggttgaagt
    28021 gctttataat cctctgtaga aatacctttt gcaaatttat ttgggtcata attaggataa
    28081 cttgatgaag ctaatacttc tccattttta acatcaatag ctataagagc tgctgactgt
    28141 gcttttcctg catatgcact aattggtttg tccccaaatt tacttttaaa agtacctcct
    28201 ttagaagcaa cttcgattat ctgttttaat gcattatctg aaacttcttg taaatcttta
    28261 tctagtgtaa gatacacagt atctcctgat ttaggttttt tagattcaat ttctttactg
    28321 attctaccca atgcatcaac tttaaccatt ttataaccat cagttcctct taatctactt
    28381 tcattactct tttctactcc tgcaagacct accatatctc cagtttcata acctttttga
    28441 aggtatgatt ctatttgagt ggatggcatt tttcctacat atcctaacat atgagaagct
    28501 aatgacccat taggataata tcttactggt tctactgcta ttgaaacacc aacaaggtcc
    28561 atagcacttt cttcaatttg agcaatcgta gtttctccta catccttagc aatagttact
    28621 ggattatatt gagaatatcc ttttgatttt atcaaatccc ttacgaccat tatttttctt
    28681 gcatcttcat cactcaaact tttatcaatt tctaatgcat cactatttct tactttttca
    28741 aacgcttctt tggcactcag tttagtttct tttatcttat aactagccaa ccaatctctc
    28801 ttatctcttt ctgctgtgaa catccattta ctaactaaaa taggtggata atatccattt
    28861 tcatttagtt ttgcctgtaa tcttgtcgca tccaatcttt tatcttcttg agataatatt
    28921 ccagctgata taagtttgtc tactaagtaa taaaaacttt ccttagcatt ataatcattt
    28981 ggaataccat tttctgattt atattcccta atatctctgt catatgtata atagtacttt
    29041 ccattttcta catatatagg aaactcatct acatattctt caccatttct ctccagcaaa
    29101 tttataagtt ttaatgatat ttcatttgct cttgaatgct tatttgcatc tttcttatta
    29161 atatcgttac ctgaaacttg aacagtaaat aagtttttat ttcctgccaa cagtcttcca
    29221 tatctatctt ttatttctcc tcttggagct tctattgcca actttttata tgttttattc
    29281 tcagctaact cattgtaata ttcatattta aatgtagtca tatagattat ttttactaaa
    29341 atagctaaaa atatcacgct catgactatc ttaattattt ttagtctatc aacttgttta
    29401 ttttcttcca tgaaatcacc aactaatctt ctttaagttt taaaactgct cttttagata
    29461 agcgatatat tactaaacca attaaactat tcactattgg tgctactagt agacctttta
    29521 caaaaagtgt tgatatgttg tagctttgat aaattatact agatgtacct atattgacaa
    29581 ttgaatcaat taaactgcct ataaaaatta aaacgaaagt aataatataa ctacctttat
    29641 atagctgatc tctcatgtat ccaattgcat atgcaataat aaacaaaatt aatgcattta
    29701 caccaaaaat tccaccaacg gttatgtctt taataagccc tattattgct gctataatcc
    29761 caccttctaa ttcatctaaa taaagagaga ttatggttac atatataaga actaaattaa
    29821 agcttatgcc aaaaatatct atataatttg ttatactatt ttctatagtc ataagcacaa
    29881 tccccaacag acaaagtaaa acttttttca ttgtttttat cccctttatc ctatatttct
    29941 tggttctatg actacaacgt catttatatt cttaaaatca acataaggtt ttacaactac
    30001 atattttaat gacttatctt tatcatcaac caccttttca acttcgccaa ttggtatacc
    30061 atctgggaag aatccaagac ctgatgttgt tattatatct ccttgaatta cattatatga
    30121 tgagtcaaac atatatcctt gcaaatatcc tttactatta taactatctt tattatctaa
    30181 agtagtattt tgagtaattg tacctttagc attggcatcc tttgctagtt taaagcttac
    30241 agatgcctta gaatccaata gtgaaattgc cttactataa ttgttggaaa cttcataaac
    30301 tatgccaact aacccactac cattcataac tatactttct ttttttacac ctgaattttt
    30361 tcctgcacct atgacaaaac tctcatacca gtttccatca tttttaccaa caacacttgc
    30421 tgaaatactt gtcgccttat actcttcttg tacaaagttc agtgtctttt ttaactcttc
    30481 tagcgattct gttttatcta gtttggcatt taaagctatc atatcttttt taagtttttc
    30541 attttcttct tccaattttt ttaccttttt agaattatct ttaaaatttg ctacatcttt
    30601 aaaaccattt ttgataaaag aaaatccatc atttattccc ttacctacac ttgtaatagc
    30661 atctgaagct actcctaaat ttactggact accactagaa aatttaccaa ttgatatacc
    30721 cacaattcca attaaagtga tagcaactac gcccgttgct atcactttaa cattaatcct
    30781 ttttttctca tttttgttct tgtcaaatct caaggccatc accaacttct aattcttagt
    30841 attcatcatt aatacttttt caaagatttc ttggtcttca acagatttac ctgttccaag
    30901 tgcaacacaa tcaagaggtg tttctgctat ttgtacagac ataccagttt cctgtgtaat
    30961 aagcttatca agacctctaa gcaaagcgcc tcctcctgtt agcattattc cgttttccat
    31021 tatatctgag gctaattctg gtggagttct ttctagtgta gattttattg catctactat
    31081 tgaacttaca ggttctttta atgcttctct aacttctgta gagcatattt ctattgtttt
    31141 aggtaatcct gagattaaat ctctacctct tatttgcata tttatttctt catcatcttt
    31201 aaatgtagaa cctatgttaa tttttacatt ttcagcagtt ctttcaccta tcataagatt
    31261 atattctttt tttacataag ccactataga ctcgtcaaat tcgtctccac ctattcttat
    31321 agatttagca gtaacgattc ctcctaagga tattacagct atttctgaag tacctccacc
    31381 aatatcaact accatattac cttctggttc ttcaactttt aatccagccc ctatagccgc
    31441 tgccattggc tcttctataa ggaatgcatc tttagctcca gcttgtcttg cagcctcttc
    31501 tatagctctc ttttctactt ctgtaactcc aaaaggaaca catatagcta ttcttggact
    31561 tacaacacct tttttatcag ccgctttttg tataaaatag ctaatcattg attgagttat
    31621 gtcaaaatca gctattactc catctttcat aggtcttata gctactatat ttcctggtgt
    31681 tctacctatc atttttttgg cttcttcacc tactgctaat acctctttac tatcatctct
    31741 tattgcgaca actgatggtt ctctaacaac aataccttgt ccttttatgt aaactaatgt
    31801 atttgcagtc cctaaatcta ttcccatatc ctttgtcatc ttattaaaac taaaaaaacc
    31861 ttttttttct ttcttttttt ccttagccat gattttactc cttcctacta ttaagaatca
    31921 tgtttaaaac attctcctat tattttatca catataaagt caaattatca tattttcttt
    31981 aaagctaaag tataatccat ctccaataat tatatggtct attatttcta ttccaattat
    32041 ttctccacat ttaataagtc ttgatgtgat atttttatct tctcttgatg gttctacact
    32101 tcctgagggg tgattatgca tcactattat cttacttgca cttcttctta tagcttcttt
    32161 aaaaacctct cttggatgga ctaatgacga gcttaaagtc cctatagata catctacatc
    32221 agatattatc tcattttttg tgtttaaaag aactgcttta aaaacctctt tttttaaata
    32281 ccttaggcta tccatataat acctctgtat atcccaagga ttccttattt tatactttat
    32341 aggtttaaaa ctagctactc tagagcctag ttctaaagct gctttaattt gggttgattt
    32401 tgataaccca attccatcta tattacacag ctcctctatt gtcatatctt ctaaatgtct
    32461 aataccctga atatctctat ttattatata gttggctaat tcaatagcat ttttattctt
    32521 gttgccagtt cttaaaagta tagccaacaa ctcagcattt gataagcttt ttacgccttt
    32581 ggcaagcatc ttttctctgg gtctttcctc tggtgccatt tcttttacct taatggttga
    32641 attaaatgac tttttcagat aaatcacact ccataaaaaa gatttatact aaaatatttt
    32701 tttaataggt cgcctaacct tgaaattgga agaccaacta tattaaaata atctcctcta
    32761 atttcttcaa ctaacaaagc tccataaccc tgtataccat aggctcctgc tttgtcaaat
    32821 gactcattag ttttcagata atcctcaata tcctgctctg ataacgtttt aaatttcaca
    32881 ttgctgatta catagtcaat taccttttta tcttcgcaca agtttattaa gctaatacct
    32941 gttatgactt gatgctctct tccagatagt ctttttaaca tatctcttgc acaagactca
    33001 tcttttggtt ttcctaaaat agcattgtct aaaacaacaa ctgtatcagc tccaataaca
    33061 acatcttcat tatgttcaga tgctacagac atactttttt caaaagctaa tctcataact
    33121 aaatgttttg gtgtttcacc ttccaatata atctcatcaa tttcattttt aattatatca
    33181 aatcttacat tcgtattctc tagaatttct ttccttcttg gagaggctga tgccaagatt
    33241 atattcattt attcacctca tttgtacgta atttgtacct atttagtcta tcactatatt
    33301 gtaaaaaatt caacagaaat gtcgaacctt ttttgttaca ctattgttat atttattacc
    33361 attagaataa atatacataa aatagaggta tggaaattat tccatacctc tgttttatgt
    33421 actcattaga gatatggaaa ttattccata tccttatttt gtccatatta caaaatttta
    33481 atagctttct ttggacattt ttccatacat aaatggcatc ctacacattt atctgcatct
    33541 accttatgtt tttcttttaa ttctccctct attgcatcaa acttacattg ttttttgcac
    33601 actgtacatc caacacaaag ttcttggtca atagtaactt tctttttctt agttatgtca
    33661 cctgatatta cttttgtagg gcattttgct acacattgca tacaccctac acacttatta
    33721 gggtctatct tagctatttt gtcttcaaat attattgcat caaatttaca agcttttaca
    33781 cacattccac agccaataca tccagcagta catttttcct taacagcttt accaaactct
    33841 ttgctattac actctacaac tacttcttga ctttctggtt ttgtgattat tataccctta
    33901 ggacaaactt ctttacattt tccacaattt acacactttt cttcatctat tactgctatt
    33961 ccatcaacta tagaaatagc atcaaattta caagcatcct tacatgttcc aagacctaaa
    34021 catccaaatt tacacatctt agcacctgag tttaaaacat ttgctgctct acaatctgat
    34081 attccttcat actcatattt atcttttgca cttgaacaag ttcctttaca aataactttt
    34141 gctacttgtt tctcacctgc ttcagcactt attcccatta tttctccaac ttttgcacca
    34201 acatctgctc cacctacagg gcaaccatta actggtgcat tgccttcaac tatcgcattt
    34261 gcaagacctc cacaacctgg aaatccacaa cctccacagt tggctcctgg aagtactcct
    34321 agtatagctt ccactctctc gtctacttct acagcaaact ttttagatgc gaaatctagt
    34381 actatcccga agattaagcc cataattcct aaaactaata cagctgtaag tatcaccatt
    34441 ttcacccctc ttttttatag ctttataagt cctgtaaatc ctaagaaagc tattgacatt
    34501 aaacctgctg atataagcgt tatagggaaa cctttaaatg cttctggtat atcagctaac
    34561 tctagtcttt ctcttatacc tgcgaatatt actattgcta aagtaaatcc agctccggct
    34621 ccaaatccat ttattattgt ttctactaaa ttataacttt tttgaacatt aactagagct
    34681 ataccaagaa ctgcacagtt tgtagttata agaggtaaat aaactcctaa tgcttgatat
    34741 aaagatggac tcatcttttg aattaccatt tcaacgaact gaactataga agctataacc
    34801 aatataaaag ctattgtttg taagaatcca gttccagttt ttactaataa aatctgtata
    34861 aaataagtta taattgaagc tagagttaaa acgaacgtaa ccgcaactcc catccctact
    34921 gctgtatcaa ccttcttaga aactcccaag aaaggacata tacccaagaa ttgagatgtt
    34981 ataacgttat taacaagaac gatacttaaa aataaaagta ttaaattcat ttattacacc
    35041 tccactatgc ttttttactt cttaatttat taaatcctgc aaataagaat cctaatgtta
    35101 agaatgcacc tggtggtaat ataaatatta atactggttg gaaagatgct ccaaataatg
    35161 tcattccaaa taaactacca tttccaagta attctcttac tgcacctatt attgtaagtg
    35221 caacagtaaa tccaagacct tgacctacac catcaactat tgaaggcata gctcctgttt
    35281 tgaaagcaaa actttctgca cgagctaata ttaaacagtt aacaactatt aatggtatat
    35341 atataccaag agccttatca agagcaggaa catatgcttt taaaagcata cctactattg
    35401 taacaaatgt agctataact actataaata taggtattct tatcttatct ggtgtaattt
    35461 ttcttattaa agatataact aaatttgcac atattaaaac tgctgctgtt gaaagaccca
    35521 taccaattcc atttattgct gaagttgtaa cggctagtgt tggacacata cctataactt
    35581 gaacaaatgt tgggttctca tctattagcc catttttaaa tactttagct aaattcataa
    35641 ttaaccctcc ttatttatta agagcactat tgaaaacatc tatagcagca tttactcctg
    35701 tagtaactgc tgtagatgta atagttgcac ctgatattgc taatatttga ttatctgctg
    35761 ttgtacttcc ttttgctact tcaagaggct cagcagtttt tcctttgtat tgacccttaa
    35821 atgcatcatc cttagctttt gctccaagac ctgctgtttc tgacatatta ccaatgtcaa
    35881 cacctgtaac ttgaccttca gatgatatac ctaccataac ttcaatttct ccaccatatc
    35941 cacttggttt agcttttaaa gtatatccaa ccacttcaga accatctaaa ccttcataca
    36001 cttcagctat aagaccatct ccaaggtctt tatatgtact tttatccatt tctttaaatt
    36061 cttttgcttc tggaaggact gcttttctaa gttcattatt ttcttgtata tttctttgtt
    36121 ctattactgg agctgtaacc tgattagtag ctccaagagc taatgcagct attgcactta
    36181 tagcaagtaa agtaccacct aatctcacca tactgttcat tatttagcca cctccccaaa
    36241 tactctattt ctaacaaact tatctataag tggagcaaga cagttcacta atagtattga
    36301 gtaagataca ccttctggat atccaccata cattcttata acagttgtta taagaccagc
    36361 taatacagcg tatattattt gacctttttt agtcataggc gaagttgtat aatccgtaag
    36421 catgaagaat ccacctaaca taagtcctcc agcaaacagt tgatatattg cgaaagttat
    36481 attaaatcca cctaaaagga atgttaatat aaatacagtt gctatgtaaa atactggtat
    36541 aacataattt attatgcctt tatacattag gtatactcca cctaataata ataatattgc
    36601 tgatgtctca cctatacatc caccaatatt ccctatcatc atgtcagcaa gactaattcc
    36661 attgctagaa aggtctgcca gtcctgctgc tccatttttc aaaaagctta atggagttgc
    36721 tgtagtaact gcatctaaat ttttacctat ccagttaaca cctggttgag tccaagcagt
    36781 cattgctact gggaatgatg ctaataaaaa tgctcttgca gcaagtgctg gattgacaaa
    36841 gttacatcca attccaccaa ataccatttt aactactaat attgcaaata ttcctcctac
    36901 tagacacatc caccaaggaa gtgatgctgg tacgttaaat gctaataata atcctgttac
    36961 aacagctgaa aaatctccta tagtactttt ttgcttcgta aatttttgat ataaaaattc
    37021 agtacctact gtaccaagta tacaaaaaaa catagcactc aaagcattaa gtctaaagaa
    37081 gtatactcct gctactgctg ctggaaggag tgctataata acttgtttca ttatatatga
    37141 agtatcttca ttacttctca catgaggaga agatgatact atcaacttat tttccatcgt
    37201 ttttccccct taactttctt tatttctgag cagcttgctt tcttctcttt gctcctattt
    37261 ctctctttgc tgatcttata gattgaagta atggtcttct tgctggacaa ataaatgaac
    37321 aagacccaca ttctatacaa tccattgctc tatgatattc agctgtgtca tagtcatctt
    37381 ttaaagaata tgcacttatg aaaagtggtt gtaagaatga tggacataca tctgtacatc
    37441 ttccacatct taaacagttt tgaatatctg gagtacgtga ttcttcctca tccaagcata
    37501 aaattcctga agaacctttg ttagtcgcta tttcagtagt atattgagct attcccatca
    37561 taggtccacc cattataacc ttaccaatct tcttatcttc tttaaatcca ccacattgtt
    37621 ctattatttc tgaaactaat gttccaactt ttgttattag gttctttggc tctttgatgc
    37681 aactacctgt tattgtagtg attctttcaa ccaaaggcat acctgtttta atagacttag
    37741 ctatttgtgc tgctgtggca acattgtcaa caactgcacc tgctgctatt ggtaatccac
    37801 cagatggaac ttctttgcct gtacacgcat aaataagttg tttctctgct ccttgtggat
    37861 atttcacttg aaggccaaca acttcgattt cactatagtc tttagctaca ttttgaattg
    37921 cttctatagc atctggttta ttcgtttcaa ttcctataaa tccttttttg acatctaata
    37981 ccttcattaa tgctcttaaa ccaaacacaa catcttctgg attttcaacc attaatctgt
    38041 ggtctgcagt taagtatggt tcacattctg caccatttaa tataactact tctgccttgc
    38101 tatctggtgg aggagatact tttacatggt tagggaatgt agctccaccc atacctacta
    38161 ttcctgcctc ttttatgata cctactatat cttctttgct caaatcctct aactgacctt
    38221 ttggttgaac actctcatgt aattcttcct taaagtcatt ttcgataact acacattgag
    38281 ctgaacctcc tggtacttca tgttgttcaa tagcaataac tttacctgat actgaagaat
    38341 gaacgttaca tgaaacaaaa ccttgttgtt cacctatttt ttgcccaaac tttacttcat
    38401 caccaacttc aacaataggt ttagctggtg cccctatatg ttgctgaaga ggaatgtaaa
    38461 ctatctttgg agcttgagct ttttcaagtg cctttgtgtt agagtactct tttctatatg
    38521 gaggatgtat ccctccctta aaagttaaga gtttcattgt tttaccccct tatctataaa
    38581 aaaacatttt ttctgccttc tataagtata ctatatatat taaaaaatct taacaattgt
    38641 taaaaaaata tccgtatttt tataatttat tttataaatc aatacctcta tattaagtca
    38701 aactgaggaa aaggattgct taattaaaca atccttttca tatagctttc taaagtccaa
    38761 attttctgta ttttatttta tagaatttat aaacatagag tactcctgca tggatgataa
    38821 aagcagactt ttacttacac tatatttttc ttcatttgct gcttttgaag ctgtatcaat
    38881 tttttcattc acattcttta catactctat taaattatct ttaaataaac gcatttcaga
    38941 atagtcaatc ttttcagctt cttttgatat attttgtgat attgcctttc tatttgtcaa
    39001 aatagtatta tatttttcca tatctacatt attttcagca ttcgaccaaa aacttgattc
    39061 ttctttaaag tttgttatta atttatttaa ttctttagat atactctcta tataagcgta
    39121 gttacttgta tactctaaag acaacatagg tgcatctaag tgtgataaaa atgcatctgg
    39181 aaacaccttt cttacactgc ttatctgctt tctaacatca ttttcatcaa aagaggaata
    39241 ggtctgtatt ttttttactc catccttctc cataactgaa aaaggtatgt cattattaac
    39301 taatgatgtc tgtatttttt tcaaatcatc attattatct attgcagcaa cttgaatagt
    39361 gtacatatcc cagccttcta tagtagctaa tttgacttct tctggctctt tagtgttatt
    39421 taccgctttt tctggattag tttcttgttt atcatctgaa ttggtttgtg atttagattt
    39481 tttccctttt tttattgaat ttaattcatc agatatatct ttataagtta tatctgatga
    39541 attatttaga aatggtattt tagccgatac atactccaat atcttgctat cttttatttt
    39601 tgtataagaa taatagccaa ttagacacaa gcaagcaatc gttataacat atttacgtaa
    39661 atcaatctta tgtctattat tattaaatcc tctgtatatc gtttttcttc tattcattag
    39721 tttgtccctc ccaaaattat attattaata aagtatattt ttaagagtca caatttatga
    39781 atataaataa ttatttcttc taatttttta ataagaaatt gtacttacta aacaaatctt
    39841 cttgcaccaa gtaatacttt agaccaatat gaactagtta aattatccac tttaacctta
    39901 ttaggattag tctgagtacc actacaatgg ataaatttac tatttcctac atatatacct
    39961 acatgattag ttgttccatc accatcagtg tcaaaataaa ctaaatctcc tggtgcataa
    40021 ttccccatag aaattgcact tccaaaatta gcctgttctc tagaaactct aggaatagaa
    40081 acgcctacag aatgtttaaa aacatactgt gtaaatccag aacagtcaaa tttatcaggt
    40141 cctgaagctc cccattgata tggtattcca ataagagtat atgcaaaatc tacgattgtt
    40201 gaaccttttg ctgtagattt atctgaattt ccagtaaatg atggctttga gttactatta
    40261 gattgagaat tttggttaga tgatgaattt ggtttttctt ttgtaccttc attagtctca
    40321 gaaatataac tggcactcac ccatccaatt gttccactcg ataactttac cttataccaa
    40381 ccattatttt tggattttaa ttcaacaaca tctccaccat ttaattttcc tattatagaa
    40441 tagcttgtcc caggaccact tcttacattt aaactcacag cagatgttac cttaccattc
    40501 ttgccttcaa ttgattcttc gctagatggt tttttatcag aattattagt actatttgaa
    40561 ttactagaat tattcgttcc aacatcctct gatgttttag agatatattg gctactagcc
    40621 catccagtaa ctccatttga tagttttatt ttataccatc cattgctttg ttctagtaac
    40681 tttactacat ctccattatt tgctttacca actaatgaat agttagtacc tgcaccactt
    40741 cttacattca gtctagaact cacatttact ttaccatttc cactaatagt tgtcccactt
    40801 gaagttggat tattttgatt tgatgtacct tcagatgaac cacttgttga tatgtatttt
    40861 gcgcttcccc atccaacaac agaactcgat acccttactt tataccatcc attagacttt
    40921 tctaaaatct ctacagtttt tcccttgtat aattttgcta ccttatcaca atcagaactt
    40981 ggtccacttc ttatattcaa ggcacttgct gttactgttc ccttttcaag agcacttgca
    41041 tttatggagc taacagatac tgctactgcc ccaattccta aagctgctat agctttcttc
    41101 actactatca ctccccccaa caaattttta tagtcataaa ttcataacat tattatccat
    41161 ttttagtccc tacaatttaa tgataactta ttctcttatt aattgtaaag aaattataaa
    41221 catatcctta aaattaatga tttttaggaa gtatttcaat ccaataatta tctgggtccg
    41281 ctataaaata aattcccata ctttcatttt caaaacatat gcaatctaaa tctttatgaa
    41341 gtttttttgc ctcatcaaac tcctcaactt ctaaagccag atgaaattca ttgtctccta
    41401 aattatatgg tctgtcccaa tctctaagcc aagttaattc taatgtatga ctagtaatac
    41461 catctcctaa ataaactaat ataaaacttc catcttctgc ttccttacgc tttacctctt
    41521 taagccctaa agctttttta taaaaatcta aacttttttc taagttagta acattgaaat
    41581 tgttatggca aaaattaaac ttcattttga aatcactctc caatttttat tttttttata
    41641 ttatatcatt attataaatt tatttaaatc atattaagtt aaaaatttga atgatgatga
    41701 ccaattacca aatggtggta aattatttaa aagaatatgt caaaaaaaga ataagctctt
    41761 tcaaatgcac aaaaaattta tacccaagtt atacttaatc atgcaaattg gcagaactta
    41821 ttctttaaaa ctctaacttt taagtttttc tttgataata taatttatta ctttttttct
    41881 aatatcttca gcattataat catcttctac ttctatatcc aaaagttttg ctattgacct
    41941 catatcttct ctagtctttg acctatagct tgatattaat tcatctttta ttttctctgg
    42001 ttctaaaaga tattcttcat ctcctctttt gaaaacaacg tatttttgag catatgaact
    42061 tctattatag tatatatatc ttgatatttt tcttaaaatt tgattatatg aaagtcgctt
    42121 gttaatatca agttttaagt actctgaaaa agcaaatagc tctttttttg atttaaataa
    42181 tgcttctttc tttatagtcc ttgatattga agttacactt tcttgatatg cacacttttt
    42241 taacattttt aaatatactc tattaagacc aatactttga ttgaattttt ttatattatt
    42301 attaataatc ttatccaagt taccttcaaa gtattttttt gctccttttt gtgatacata
    42361 actctcaagt tctttgtggg ccatatcaag tatcattttt tttaaatcct ctttatatcc
    42421 cctagattcc atgatactcc ctcccttagc tatttatagt atattcaaaa agctcaagaa
    42481 atgtgagtca ttacaaaatt tttttacttt ttatgtatat attaatttaa aaggggagat
    42541 aatagtaatg taaaatcaaa catactcaat ctcccccatt tttagacctc tattactccc
    42601 ccaatagagg ccttttttta gtttatcaat ttctattcaa ttccctttag ttcaaatcct
    42661 aattctgcta tcacttcttt taatttttca atagatatat catctttcat atctacagat
    42721 gcacacttat tttctaaatc cacatctaaa acttcaatac catctatatc ttctgttaat
    42781 gcagttttaa gatggtttac acaatgacca caactcatac cttctactaa tagtttcttc
    42841 ttcatttaat tacctcctta taccccataa gggtattcta tcatatagtt tatacttttt
    42901 aaatagaaat taatcattta atttgattca ttcgatttta tatctaaatt agatattccc
    42961 tcatatacat caaaatcatt tggagtaatt ttacctttta atctactatt acttacaata
    43021 tagttgtttc tcacagctaa acttataaat gttgcattat cttttatata tgtctgtaca
    43081 tctttgtatg ctttttttgt ttcaatttca gacgtagaac ttgtaagtgc agacatatat
    43141 ccatttagct tatcatcagt atatccacta cttgctataa tactacttgc atctggtata
    43201 attgatagtt tccatccaac taatgctaaa tcataatttt tagaattaag agcattttcc
    43261 atatccttat ctgacagctg atttactgta ctatttattc ctatagcttt taagttttca
    43321 tttattatgt ttgcagtttt tattctctca ctattatctc tgtttactat tatctttaag
    43381 ttcatttcag aaagactttt ctttacttcc tctttttctc tatttttttt cttttcagct
    43441 tcttttttga ttctatcttc ttctgctttt ttagcttttg cttcttcttc tggcgtaagc
    43501 ttctttgagt tctttttagc tgattttttc tgatttttta ctttattgtc attttgattt
    43561 actgagttta tatttgcata ttctacctta gcaagacact ctatcgcctt atctttatta
    43621 aactctaaat ccttcatttc actattatag tattttgatt ttgaattaag tggaaaatta
    43681 actggagtag catcatccat ataaccttca ctaataattc tatctttatc tatagagtga
    43741 gcaattgctt ttctaaaatt aacatcttta aaaaatggat tattataatt aaataataca
    43801 cactcataat ctcttccttg atattttgtt atattaaact ctttttcttg aaacttgctt
    43861 aaatcattta atgtaacatt ggttatatca ctatcaagag ccataaccat agatgtcctt
    43921 gcctcttcat taggcaccat acctactttt atgttcttca tggtctttgg aacttcttca
    43981 tagtaatctt tgtttacaga taaaaccatt ccttctcttt ctgtatatga ttctatttta
    44041 tatttcccat ttcctatcat attattattc ttgtcattta catcctttga atctaaatga
    44101 ttttgagata cgattggaaa aattaaacta tcaattgaaa aagcatatct agctttaaat
    44161 tttattgtaa aatctttatc tgatttaata ttcacagaag ctatattttt agtaaattca
    44221 ttatatggac tatctgcact tttttgaatt aaaccaatag tatagtttac atcatttgaa
    44281 gtaacatttt ttccatcatg ccactttgca tcttttagct ttatatctat actcattcca
    44341 tcctgagcta tattatactc cttaactaat tgaggaatta cattgtagtt ctcatctata
    44401 gtaaataaac tatcataaac taaattcatt atataaccaa cagacttatc agtattagtt
    44461 ataggattta tagtttttgg agttaccatt gttaagttta tatacttagc atctatagaa
    44521 ctttgacttt gtaaacttga atcactacta ctttgttttg ttttagtatt actacaacca
    44581 gctaccataa gagttatagc taaaacgact gttaagactt ttattctctt cattagtttt
    44641 ttcctttctt gccatatatc ttattataat gttcgtaatt attttttact ttttctacat
    44701 attttgaagt ttgcttaaaa ggtatatcat gtagattttc gccatcttta ctatattcat
    44761 tattttctaa ccattttttc acattacctg aaccaccatt atatgcagct atcactaaat
    44821 ctaatttacc aaattctttg tataacttac ttaaatacca acagccaagt cttatattag
    44881 tctctgggtc gaaaatatcc acatttttta aatttagttc ctctgctccc caatctctag
    44941 ttatatctaa tatttgcatt aatccttttg cttccttttt tgaaacagca gaactattaa
    45001 acttactttc ggctttaata acactgtaaa ctatattttc atctaaatta aattcttttg
    45061 aatacttttc tacatactct gaatattttt taggatataa aaatttatgt attactttgc
    45121 tttccattaa tagtgcccca aataagatta taaaaataga tagaatcaat actttcttac
    45181 tattcacgcc aattctcctt catgtactca ataaatttat gtgctttact ttctaattca
    45241 gttattgttc ccgaattatc tattatataa tcaccatatt tggatttttc ttcctgactc
    45301 atctgtgaat ttatcctact aagtgcttcc tgcttactac aattatctct cttttgtatt
    45361 ctactaattt gtacttcttg tttgcaagta actactaata gcttgtcaac catatctaaa
    45421 aaaccacttt ctaccaatat tgctgcatct aatactacta tattttcgcc tttttttctc
    45481 aattcttcta tttcacttat tattttttct cttattcgcg gatgagtcaa gctatttaat
    45541 tccttaagtt tttcttcatc gctaaaaact attttcccaa gagcttttct atttagagtt
    45601 ccatcatcat tttttatact ttgcccaaaa tggacaaata ctttttccaa taacaactta
    45661 tcctcaaata tcttcctgga tataatatca gcatccacta tagaaatatt aagatttcta
    45721 aatatatttg ataggctgct ttttccacag cctattcctc ctgtaagtcc taatatcaac
    45781 ataaaatcac ctactttgtc tcataccaag aactacctac atttaaatct acatctaagt
    45841 gaacatccat atttacagca ttttccattt cttcttttac tattttctta actatatcta
    45901 tttcatcatc aattgcttca actatcaact cgtcgtgaac ttgtagtata agttttgatt
    45961 tcaaattgtt ctcttccaat tttttatgaa catttatcat agcaattttt attatatctg
    46021 ctgcacttcc ttgtattggt gcattcattg caaatctctt tcctctattt ctttccataa
    46081 aattgcttga ctttatttct ggtatatatc ttcttctgtt taaaattgtt acagcatatc
    46141 catcctcact agccttttct actgttgtgt ccataaactc ttttatttta tggtatttag
    46201 caaaataact ttcgatatat tctttcgcct ttggaacagg tatatttaaa tcatcagcta
    46261 gaccaaagtc acttttacca tatataatac caaagtttac agctttagct gcacctctca
    46321 attcactagt aacatcttcc ataggaacat taaaaacttg tgaagctgtt ttggtatgaa
    46381 tatcttcatg atgtttaaat gcttctatca tattttcatc ctgactcata tgtgcaagaa
    46441 ctcttagctc tacttgtgag taatcggcat caaccaactt acagttatca tctgcaataa
    46501 acacttttct tatttttcta cccatttcca tttttatagg tatattttga agatttggtt
    46561 ctgtagatga aattcttcct gtagtcgtta tagtttgatt aaatgatgaa tgtattctat
    46621 tactttctgg atttattata tttaaaagtc cttcaacgta agtagaattt aactttacta
    46681 tttgtctata ctcagttatc ttatctatta ttggatgctt atctcttaat ttttctaaaa
    46741 catctgcatt agttgaatat ccagttttag tttttttaat tattggaagc tctaattttt
    46801 caaataatat aacacccaat tgttttggtg aatttatatt gaatggctct cctgataatt
    46861 cataaatttc cttttctagt gtagctatta tttctttaaa ctctacactc aattcatcca
    46921 atatactttt gtctactttt ataccttcat actccatatg acctaagact tctactaaag
    46981 gcatctctac atgataaaaa agaccatcca tactcatatc cattagactt ctttccatca
    47041 taggaattgt ttctttaact gtgcatatta tttgacccat atactctgct aaatcctcaa
    47101 attctaaatc ctcatagttt ttagatttaa caccctttcc taataattct tcttttgatt
    47161 taaccttttt tgataaatac ttcatagcta ttgcactaca atcatatgag gtagatgaag
    47221 ttgaatctat taagtactca gctattgtta tatcaaaata catattttct aagtttatat
    47281 tatatggttt taaagatata taatcatctt ttaaattata tccatacttt ttaatttcat
    47341 tatttgaaaa tatactatct aactttgaca cttcatttga atctacataa tatatttttt
    47401 ctccatctac acttataaaa atatatatta tatttttttc aagtatatta ccttgtcctc
    47461 taacagtttt taaaataact tgtccttttt tgtttacttc atctataaat ttttctgtat
    47521 cttttaattt taatatttct attttttctt ctacgtgttc tgtgtcatct gaatcgacca
    47581 aatcaactaa tcttgataag agacttgtaa atccaaagta tctaaattta tctaaaagtt
    47641 catctttatc ataatcgcca aaaaccatac tatctaaatc cacttctatt ggcacatctc
    47701 ttataattgt tgcaagtctt ttactttgaa tagctatttc tttattttcc tctattttct
    47761 tttttacact acctttaagc ttatctgtat tctctattaa attctctata ctagaaaact
    47821 cttttataag ctttatacct gttttttcac cgattccagg tactccaggt atattatctg
    47881 acttatctcc cataagacct tttaaatcaa taaattgtgc tggagtcatc tcgtattttt
    47941 caattacaga attaaagttg tattcttcaa cttctccaac tccttttttt gttataagag
    48001 tagttgtatt atctgaagca agttgaatag catctttgtc tccagttact atgtatacct
    48061 tatacccatt attttctgct tttttagaaa cagttcctat aatgtcgtct gcctcatacc
    48121 catctatttc caatctattt attttgaatt tatctaaaag gtcttttaat ggctgtaatt
    48181 gttctgccag ttcatctggc attccctttc ttccagcctt atactcttta tattctaaat
    48241 gtctaaatgt aggtgctttt ctgtcaaagg ctacacttat atgtgttggt ttatatatat
    48301 ctatcatttt aaatagcatt gttgtaaatc catatattgc attggtcttt aacccttctt
    48361 tattattcat ttctggcaga gcataaaagg ctctgtttat tattgaattt ccatctatta
    48421 taattaattt tttttccaaa ttatatcact cctaaaaatt atctattttt gtaacatata
    48481 tattatatac caatatagtg ttatttaata ttcccataat aataatagta tattaaaatt
    48541 aaatatgttg caaacaatat atttttttgc tataataata tacatgacat gcccgaatgg
    48601 ttgaattggt aaatacaatg gattcaaaat caactgtgtt gattcataag tttttataat
    48661 gttttacagt ttgatgagta ttttgtattc aatattcctc ttgcatattc ttggaaaatc
    48721 tggtaggata tacaagtagt ttggatttaa aatataagcc gctgtggcgg aactggcata
    48781 cgcatacgac tcaaaatcgt acgggaaacc atatgggttc gagtcccatc agcggcacca
    48841 ataaaaaaaa ctctttagta tatactaaag agttttttta ttgtaatatt ctttcatctt
    48901 tatttctagc cttgtataag tataaatacc atagtcataa cttcttgaac tcctaaaata
    48961 tatctaaaca aagtaggatt ttttatgtat tcacttaata aatccatact tcttatcata
    49021 ttgatttttt cccaatctct aaaaattttg atacaaataa aaaatacaga gaactacttg
    49081 atatactaga aggttattct gtaccatttg gtattgctgt aggtgttcca gatggaaatt
    49141 taccaaatgc tagagatgta gtttataaag tttctagagt ttaggataaa gtgttgcaac
    49201 aatcaattag ttacttgaag agttaaatca gtaatataca aataaattaa tggaagagga
    49261 agctatttaa aaagtagttt agatttttca ttgtaaaata attaaataaa gaataaggtg
    49321 ataagtattt tatttattat cttattcttt tttatttgtg ga
//
LOCUS       DAMJVX010000013        45680 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMJVX010000013 DAMJVX010000000
VERSION     DAMJVX010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45680
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.014"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(77..682)
                     /locus_tag="PC407_000677"
     CDS             complement(77..682)
                     /locus_tag="PC407_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2496152.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     regulatory      complement(807..924)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(984..2150)
                     /locus_tag="PC407_000678"
     CDS             complement(984..2150)
                     /locus_tag="PC407_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2496153.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(2298..4439)
                     /locus_tag="PC407_000679"
     CDS             complement(2298..4439)
                     /locus_tag="PC407_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDF2496154.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYNEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(4583..5690)
                     /gene="prfB"
                     /locus_tag="PC407_000680"
     CDS             complement(join(4583..5614,5616..5690))
                     /gene="prfB"
                     /locus_tag="PC407_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDF2496155.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            complement(5705..8380)
                     /gene="secA"
                     /locus_tag="PC407_000681"
     CDS             complement(5705..8380)
                     /gene="secA"
                     /locus_tag="PC407_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2496156.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMVREDLPDSVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(8512..9063)
                     /gene="raiA"
                     /locus_tag="PC407_000682"
     CDS             complement(8512..9063)
                     /gene="raiA"
                     /locus_tag="PC407_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2496157.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDSEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            complement(9616..10290)
                     /locus_tag="PC407_000683"
     CDS             complement(9616..10290)
                     /locus_tag="PC407_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDF2496158.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDIGVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            complement(10293..11345)
                     /locus_tag="PC407_000684"
     CDS             complement(10293..11345)
                     /locus_tag="PC407_000684"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDF2496159.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISKKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKTPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSNTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(11393..12352)
                     /locus_tag="PC407_000685"
     CDS             complement(11393..12352)
                     /locus_tag="PC407_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HDF2496160.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIVDFTFSVV
                     TKEESPAVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(12448..12966)
                     /locus_tag="PC407_000686"
     CDS             complement(12448..12966)
                     /locus_tag="PC407_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HDF2496161.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNEKEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            complement(12977..14311)
                     /gene="celB"
                     /locus_tag="PC407_000687"
     CDS             complement(12977..14311)
                     /gene="celB"
                     /locus_tag="PC407_000687"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDF2496162.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIVTPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            complement(14350..14676)
                     /locus_tag="PC407_000688"
     CDS             complement(14350..14676)
                     /locus_tag="PC407_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2496163.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(14701..15009)
                     /locus_tag="PC407_000689"
     CDS             complement(14701..15009)
                     /locus_tag="PC407_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2496164.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(15150..17063)
                     /locus_tag="PC407_000690"
     CDS             complement(15150..17063)
                     /locus_tag="PC407_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2496165.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNVSTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            complement(17395..18177)
                     /locus_tag="PC407_000691"
     CDS             complement(17395..18177)
                     /locus_tag="PC407_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDF2496166.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(18180..20399)
                     /locus_tag="PC407_000692"
     CDS             complement(18180..20399)
                     /locus_tag="PC407_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDF2496167.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(20663..21760)
                     /locus_tag="PC407_000693"
     CDS             complement(20663..21760)
                     /locus_tag="PC407_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496168.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSNIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(22076..23269)
                     /locus_tag="PC407_000694"
     CDS             complement(22076..23269)
                     /locus_tag="PC407_000694"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDF2496169.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(23406..23509)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            23698..24207
                     /gene="yyaC"
                     /locus_tag="PC407_000695"
     CDS             23698..24207
                     /gene="yyaC"
                     /locus_tag="PC407_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HDF2496170.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(24318..24746)
                     /gene="fabZ"
                     /locus_tag="PC407_000696"
     CDS             complement(24318..24746)
                     /gene="fabZ"
                     /locus_tag="PC407_000696"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDF2496171.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEIEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(24762..25781)
                     /locus_tag="PC407_000697"
     CDS             complement(24762..25781)
                     /locus_tag="PC407_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2496172.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(25978..26238)
                     /gene="spoIIID"
                     /locus_tag="PC407_000698"
     CDS             complement(25978..26238)
                     /gene="spoIIID"
                     /locus_tag="PC407_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HDF2496173.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(26336..27004)
                     /locus_tag="PC407_000699"
     CDS             complement(26336..27004)
                     /locus_tag="PC407_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDF2496174.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(27070..28134)
                     /gene="spoIID"
                     /locus_tag="PC407_000700"
     CDS             complement(27070..28134)
                     /gene="spoIID"
                     /locus_tag="PC407_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HDF2496175.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKSTVSYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(28221..29474)
                     /gene="murA"
                     /locus_tag="PC407_000701"
     CDS             complement(28221..29474)
                     /gene="murA"
                     /locus_tag="PC407_000701"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDF2496176.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(29495..30232)
                     /locus_tag="PC407_000702"
     CDS             complement(29495..30232)
                     /locus_tag="PC407_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HDF2496177.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            complement(30716..32011)
                     /locus_tag="PC407_000703"
     CDS             complement(30716..32011)
                     /locus_tag="PC407_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HDF2496178.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKMQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKRAVDFSANWLNVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSITPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSNSVSLEEYRNIDNTMRNKSEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            complement(32464..34296)
                     /gene="glmS"
                     /locus_tag="PC407_000704"
     CDS             complement(32464..34296)
                     /gene="glmS"
                     /locus_tag="PC407_000704"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDF2496179.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEEEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            complement(34563..35909)
                     /locus_tag="PC407_000705"
     CDS             complement(34563..35909)
                     /locus_tag="PC407_000705"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDF2496180.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILNGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            complement(36079..36636)
                     /locus_tag="PC407_000706"
     CDS             complement(36079..36636)
                     /locus_tag="PC407_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2496181.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            complement(36637..37389)
                     /locus_tag="PC407_000707"
     CDS             complement(36637..37389)
                     /locus_tag="PC407_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2496182.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            complement(37389..38468)
                     /locus_tag="PC407_000708"
     CDS             complement(37389..38468)
                     /locus_tag="PC407_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2496183.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            complement(38497..38712)
                     /locus_tag="PC407_000709"
     CDS             complement(38497..38712)
                     /locus_tag="PC407_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2496184.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            complement(38755..39432)
                     /locus_tag="PC407_000710"
     CDS             complement(38755..39432)
                     /locus_tag="PC407_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2496185.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            complement(39422..40501)
                     /gene="buk"
                     /locus_tag="PC407_000711"
     CDS             complement(39422..40501)
                     /gene="buk"
                     /locus_tag="PC407_000711"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2496186.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            complement(40523..41425)
                     /gene="ptb"
                     /locus_tag="PC407_000712"
     CDS             complement(40523..41425)
                     /gene="ptb"
                     /locus_tag="PC407_000712"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HDF2496187.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            complement(41729..42910)
                     /locus_tag="PC407_000713"
     CDS             complement(41729..42910)
                     /locus_tag="PC407_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496188.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNIDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            complement(42907..43746)
                     /locus_tag="PC407_000714"
     CDS             complement(42907..43746)
                     /locus_tag="PC407_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDF2496189.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            complement(44722..44795)
                     /locus_tag="PC407_000715"
     tRNA            complement(44722..44795)
                     /locus_tag="PC407_000715"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44759..44761),aa:Arg,seq:tct)
     gene            complement(44805..44879)
                     /locus_tag="PC407_000716"
     tRNA            complement(44805..44879)
                     /locus_tag="PC407_000716"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44845..44847),aa:Gly,seq:gcc)
     gene            complement(44890..44966)
                     /locus_tag="PC407_000717"
     tRNA            complement(44890..44966)
                     /locus_tag="PC407_000717"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44930..44932),aa:Asp,seq:gtc)
     gene            complement(44972..45047)
                     /locus_tag="PC407_000718"
     tRNA            complement(44972..45047)
                     /locus_tag="PC407_000718"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45012..45014),aa:Val,seq:tac)
     gene            complement(45053..45126)
                     /locus_tag="PC407_000719"
     tRNA            complement(45053..45126)
                     /locus_tag="PC407_000719"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45092..45094),aa:Gly,seq:tcc)
     gene            complement(45141..45215)
                     /locus_tag="PC407_000720"
     tRNA            complement(45141..45215)
                     /locus_tag="PC407_000720"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45180..45182),aa:Glu,seq:ttc)
     gene            complement(45223..45298)
                     /locus_tag="PC407_000721"
     tRNA            complement(45223..45298)
                     /locus_tag="PC407_000721"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45263..45265),aa:Met,seq:cat)
     gene            complement(45314..45399)
                     /locus_tag="PC407_000722"
     tRNA            complement(45314..45399)
                     /locus_tag="PC407_000722"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45363..45365),aa:Leu,seq:taa)
     gene            complement(45405..45479)
                     /locus_tag="PC407_000723"
     tRNA            complement(45405..45479)
                     /locus_tag="PC407_000723"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45445..45447),aa:Asn,seq:gtt)
     gene            complement(45487..45603)
                     /gene="rrf"
                     /locus_tag="PC407_000724"
     rRNA            complement(45487..45603)
                     /gene="rrf"
                     /locus_tag="PC407_000724"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        13578 a         8755 c         4995 g        18352 t
ORIGIN      
        1 caatcacaat ataggactga agttataaat aaattatggc tattcaatac atttaatttt
       61 atattttctt gtctaattat ttctttatta tatttcccat ttttttataa agtggcaatg
      121 ttactaaaga tattataaca gccttcagca cattaaatgg agctatcatc caaattacaa
      181 aagtgaataa atcatgaact tgaggattta tagctgaacc taaacctatt atagcatcta
      241 gtcccataag ttgaccataa agtggcaaca ttacaaaata atttactaat gaaccaatta
      301 tagtcataga tattgttcct aatacaaaac caatcagtac accttttaaa tctccacttc
      361 tcttatatga ataacaaagt atcattacat aagaaccacc aattaatata tttgcaaatt
      421 ctcctattcc tccagttgtt gttgctgtta ttgcttgtaa cacatttttt actacaacta
      481 ttccaaatcc aactaatggt cctagagaca ttcctccgaa tattgctgga atatctgata
      541 agtcaatttt taagaaatct ggaaatatac ctggtattgg tgcttgaata aacattaata
      601 aataagatat tgcagataaa attgacatta ctactaaagt tcttgttgaa atcatattgc
      661 ctctttttgc tgtgttttgc ataattattt tccccctaat caatatgttt atatttttta
      721 ggaaaaacaa aaaactcgaa gaccctatct tcgagttttt atggatatag tttaaacaaa
      781 ctaaaataaa cttatttatc ttccataaat atcttctctc atccagactt tactgtcggc
      841 tttggaatct caccaaatca accacctaag tagttcgcgg gctataccgc cggtagggaa
      901 tttcaccctg ccccgaagat cttctttgat tttttaattt tccttacact cttatattag
      961 ttaatttttt aataattgtc aagttatttt ttctcataca ctactttgcc atttattata
     1021 gtgtaaagta cattactttg aatctctagt ggagaattat cccaaattac taggtctgca
     1081 tctttaccaa cttctataga gcctactcta tcttctattc ctaaagtctt agctggattt
     1141 atcgtaattg attctattgc tttttcttct ttcataccat gttttactgc aatacctgca
     1201 catattggaa gatattgaac tggtattact ggatggtctg tcattataca tacatcaaga
     1261 cctgcatttg aaagtattcc tgctgtatta aatgtcaaat ttctaagctc aaatttagac
     1321 ctttctgaca aagatggccc tactattaca ggaaaccctt cttccactaa ttcatctaca
     1381 ataagatgac cttctgtaca atggtctaat gtaagcttta aattaaattc ttttgcaatt
     1441 cttattgctg taaacatatc atctgctcta tgagcatgtg ccttaaaagg aatttctctt
     1501 cttaaaactg gtattaaact ttccattttt atatcatatt caggtttttc acagtcttca
     1561 tcatcatgac tctcatacat atcaatatcc tctaaatatt cttctgcttt ttttaggttt
     1621 tctctaagta aagcagctat agccattcta gtttgaggag atttatcatc ctgaccataa
     1681 cagctctttg gattttctcc aaaagcaatt ttagatgcaa ctggattttt tattaccatt
     1741 ttatcaattc tttttccaca tgtctttata gctatacatt gaccacccat aacatttgca
     1801 cttccagggg ttgtacaaac agaagttatt ccaccttcaa aagcttcttt aaaagttcta
     1861 tccattggat taattccatc tataggattt aattgtggtg ttattgggtc agtctcttca
     1921 tttccatctg ctccttcaaa ccctatacca tcttcccata aacctaagtg agtatgagca
     1981 tctataaatc caggaaacac aagtttatta ctagcatcta ttacctctac atctaaagga
     2041 gctatcaaat cttctccaat ttctattatt tttccttcat ctataagtat atctccatga
     2101 attatcccat ttgttatggt atttatttta ccatttttta taaaaatcat tcattttcct
     2161 cctgattttc aagactatcg ttcttatttt aatatatttt cttactatat acttttacaa
     2221 atttttaaca tttatacact cgagtaatct catttacaaa tatagaattt aagtactata
     2281 accatacata aacttatcta ttttttcatt gataaggcaa ctcttttttt gtttaagtca
     2341 attccaatga cttttacatc tactatatca cctactgtaa caatactcat agggtctttc
     2401 acaaaactat tactcatttc tgatttatga actaatccgt cattttttat tccaatatct
     2461 acaaatgcac caaagtctac aacatttctt attgtacctt ttaaagtcat accctcttgt
     2521 atatcttcaa tttttaagac atcagttcta agtataggtt ttataccttc ttctcttggg
     2581 tctcttcctg gttttttgat ttcagctatg atatctttta acgtaacttg tcctacttcc
     2641 agtttttcac ttaattccct caagccaatt tcttttattt tttcatctat ttcacctata
     2701 ttcttattct ttacatcatc taaagaatat ccaattatct caatcatttt tttacatata
     2761 tcataacttt caggatgcac acctgtatta tctagtggat ttttaccctc taagattctc
     2821 ataaatccag cacattgagt aaatgcttga ggtcctagtc tttttacttt tttcaattgt
     2881 gctcttgatg taaaatctcc attttcttct ctatatgcta ttatattttt tgctatagct
     2941 ttacttattc ctgcaacatg ttctagtagg gagtatgatg cggtatttaa atctactcca
     3001 acactattta cagaatcttc aacaactcca tctagcactt cttctaatct ctttttgttt
     3061 aaatcatgct gatattgacc aacaccaata cttttagggt ctatttttac aagctctgct
     3121 aatgggtctt gtaatcttct agctatagat atagctcctc ttattgatac atttatatct
     3181 ggatgttcct catttgcaag ttcagatgct gaataaacag atgctcctgc ttcactaact
     3241 atcacatact gcacttcgct atctatttct tttatcattt ctgatacaaa agtttctgat
     3301 tctctagatg cagtaccatt tccaattgaa attatatcta tatcgtattt ttcaatcaat
     3361 ccttttaaaa ctttctttgc tccctctaca tcattttgag ggtctgttgg atatactgtt
     3421 gtataatcta gtagtttacc atttttatct actacagcta ttttacatcc agttctaaat
     3481 gcagggtcaa atcccataac tactttgtct ttaactggtg gttgaagcaa taaactttta
     3541 acattctttc caaatacact tatagctctt tcttgagcta tttctgttaa atgatttctt
     3601 atttctcttt ctattgatgg gaaaattaat cttttataag aatcttctat tgaacttacg
     3661 atttcttctt tatttttgaa atttttatca tttacatatt cgtttattat gtaatttaaa
     3721 actttatcat tatttatttc taacttaacc tttaggaagc tttctttttc tcctctgttt
     3781 attgccaaaa ctctatgtgg agccatactt tttactgctt cgttataatc ataatacata
     3841 tcataaaccg atttctcatc agtagcactt ttgctaacta tcataccttc tcttaaagct
     3901 aattctctta tatactttct aatcttagca tcatctgaga ctagttctgc aattatatct
     3961 ctagcacctt ttagagcatc ttcaatagaa agaacttctt tctcttcatc aagataattc
     4021 tttgcctcta tttctatatt atctaaatta ttctctagta ttgacaatgc taaattttct
     4081 aatccttttt cttttgctat tgttgctcta gttcttttct tttgtttata tggagcatat
     4141 atatcttcta cttcttgtaa tgtttttgct ttttctatat tttgtgttat ttcatctgtt
     4201 aatttccctt gttcatctat tagtctaact acatcatctt ttctactttg aagatttctc
     4261 aaatacatta atttttcata aaattctctt agtgtaacat cactcatttc gccagtcatt
     4321 tcttttctat atctagctat aaatggtatt gtatttcctt catcaataag ttttaatgta
     4381 ttatttattt gttcatctct aagattaaac tcttttttta agatttgatt tatatccaat
     4441 ttattcactc ctaatgttag tttttcataa gtataacaaa acagaacgaa ggctgagggt
     4501 tatcgtcctc agcctaagtg ctgtaagtgc atattaagca ctaaccttct tatacatata
     4561 tcttaattac agtgcatatt tattaaacta ttttattcat ttttaagtat tcattaataa
     4621 acaagtctat atttccattc attacactat ctacatttcc aaactcagca tttgttctat
     4681 ggtccttaac caatttatat ggttgaaaaa catatgacct aatttgacta ccccaagtta
     4741 tttgtgagta tttgccttgt atatcttcta ttttttcctt ttgctctaac tcttttaatt
     4801 ctatcaattt cgccattaaa agtctcatag ctctgtcctt atttagatgc tgagaccttt
     4861 cattttggca ctgaactact acaccagtag ggatatgtgt tattctaact gctgaatcag
     4921 tagtatttac atgttgccct ccagctccag aagctctata tgtgtctatc tttaaatctg
     4981 acggattaat ttctacttct atattttcat ctaactctgg tgttacatct atagatgcaa
     5041 atgaagtatg tcttttccct gatgggtcaa atggagatat tctcactaat ctatgaactc
     5101 ctttttcact ctttaaatac ccataagcat ttgttccttc tacaagaatt gtagctgtct
     5161 ttatccctgc ttcagggtct gatattatat ctaaaagctt aactttataa cccttacttt
     5221 ctgaccatct tatatacatt ctcaaaagca tttgagccca atcttgagca tctaagccac
     5281 cagtaccagc atttatagat aatatagcat tatttttatc atactctcca tttagaagag
     5341 tctttatttt catttcagat aatttttctt ccattgattc tatggacttt tctatatctc
     5401 tttctacaga atcatcatct tcttctaaac ctatttcaat taatacttct atatcttcta
     5461 ataagctttt taagctttca tattcttcta atgtctcttt taaagattta ttttcttgaa
     5521 gtactctttg agcgacttca ttatcattcc aaaagtcttg ttgattcatc ttttcttcat
     5581 taatttgtat atttttcatc aatgaagcta tgtcaaagag aaaccctcaa ttcttctaaa
     5641 tttgaagcca tctgctccat actatgtcta tactcttgta actttaacat ttaatcctcc
     5701 gagattatct tccacaacag tttttatatt ttttaccact tccacaaggg cataagtcat
     5761 ttcttccaac cttttcatct tttttaacag ttttacttgt tggaagtgtt ccatctgatg
     5821 gagaagatag attttcaaca tctacaactg cttttctttc cactggagtt tctattgtta
     5881 tattaaataa atatcttaca gtgtcttctt tgatatgttt attcatttca tcaaacatat
     5941 caaaaccttc cattttataa gcaataactg ggtcttgctg acctacagca cgaagaccaa
     6001 taccttggcg taattggtcc atagcatcta tatggtctat ccaatggtta tcaacagctt
     6061 gaagtaagat tactctctca acttctctca ttctctcata gccaacttgt tcttcttttg
     6121 aagtatatat cttcatagct atttcataaa ctttttctgt tatttcaact ggattaagtt
     6181 tttctatttc tggtatttct atgctacctt ttggcaagaa cagattatac atatgttctt
     6241 tgaatccttc ttcatcaaat cctttatctt gtgtataaag agtaactgct tcttctatta
     6301 tagaatgagt cattgattgt atttgttctt gtaaatcttc accttctaat acacgttttc
     6361 tctctgcata tattatttct ctttgtttat tcataacatc atcatattga agtacatgct
     6421 ttcttattcc aaagttttta ccttcgactt tcttttgtgc tccttcaata gacttagata
     6481 atattctatg ttctatcggc atgtcttctt caagacctat tttatctaca ataccagata
     6541 ttctatcact accaaacaat ctcataaggt catcatcaag acctatatag aatcttgaac
     6601 tccctgggtc accttgacga ccagcacgac ctctcaactg gttatctatt cttcgagatt
     6661 cgtgtctttc tgtacctatt atagcaagtc cacctgcttc tacaacttgt ttttgttctt
     6721 cttgtgtttg ttgcttaaac ttttcaaata gtttttcata ctcttctcta gcttcaaata
     6781 gaatttcatt accttttaca ggtatacctt ctattggagt atctacccta tttactattt
     6841 cctctttaaa tccatttctt ctcatttctc tcttagttaa gaaatctggg tttccaccta
     6901 aaactatatc tgtaccacgg ccagccatat ttgtagctat tgtaacagcg cctaatctac
     6961 cagcctgtgc tattatctct gcctctttat catgatgctt agcatttaat acttcatgtt
     7021 taatgccttt tttctttaat atttgtgata ataattctga tttttctatc gatactgtac
     7081 ctacaagtat tggctgatta actttatgtc tttcaattat ttcttgtgct acagcattaa
     7141 atttacctat ttcacttttg tatacagaat ctggtaaatc ttctcttacc ataagcttat
     7201 tggtaggtac ttggaaaacg tccattttat aaattgcttt aaactcttct tcttctgttt
     7261 ttgctgtacc agtcatacca gaaagttttt tatacattct aaagtagttt tggaatgtta
     7321 cagtagctag tgttttagat tctctttgta ttttaagacc ttctttagcc tctattgctt
     7381 gatgtaaacc ttctgaatat cttctaccaa acataagtct acctgtaaac tcatctacta
     7441 ttactatttc tccatcttta gcaacataat caacatcttt cttcattatt acatgtgctc
     7501 ttaaagcttg atttatatga tggtataatt ctgtatgaga tatatctgtt atattatcaa
     7561 cattaaaata cacttctgct ttttgtatac ccgatgctgt tagagataca gccttatctt
     7621 tttcttctaa ttcataatca tctggtttta aggttaaaac aaatgtgttt gcatctgaat
     7681 ataagtgagt tgatttgtct ccaggtccag atatgataag tggtgttcta gcctcatcta
     7741 ttagtataga gtcaacctca tccactatag cataatttaa tcctctttga actctttgct
     7801 ctttgtgtat taccatattg tcctttagat agtcaaatcc atattcatta tttgtaccat
     7861 aagttatatc acaatcatat tgttcttttc taacttttgg attttgaccg tgtataataa
     7921 cgcctacact cattcctaaa aactcataga tttttgccat ttggtctcta tcacgttttg
     7981 caaggtaatc atttactgtt acaacatgta caccttttcc agtaagcgca ttcaaataaa
     8041 caggagcagt tgcaaccaaa gttttacctt cacctgtttt catttctgct attcttcctt
     8101 gatgtagtac tatacctcca atcatttgaa ctctgtagtg tctaagtcct aacactctct
     8161 tagaaacttc tctaactact gcaaaagctt ctggaagtat atcatctata ctttcaccat
     8221 ttgctaatct ttctttaaat atatttgtca tatttttaag ttccgagtct gccatagatt
     8281 caaatttagg ttctaatgaa tctattatat caacagtttt attgaacttt tttaattctt
     8341 ttttatctgc catgttgaat aaattatcca taaagctcat attttacatc tctcctcgtt
     8401 ctttattgta ctattattca ttttaccata aaaactatac ttttaatatt aatttaaaaa
     8461 gctatcgata atttatttat cgatagctaa tatgattaaa tctaaattgc tttattcatg
     8521 ttctattata ccatatccac cattattacg cttataaact actgctattt catctgaatc
     8581 tatgttcttg aacatataaa aatcatgtcc tattaattcc atctgtaata cagcttcttc
     8641 ttcactcatt ggcttaacac taaatttctt acgtctttga attactaatt cttgattttc
     8701 atccccataa tcctcttgtt catcagctag atttatctct gaatcttgga atcttatact
     8761 cttgttgtct ttatgtttat cttgtaatct ctttttatat ttttttaatt gtttacttaa
     8821 tttatcatat acaacatcta tagctgtgta taaattttcc tgcatatctt ctgctctaat
     8881 tataggacca tttacagtag ctattgttac ttctattttt tgtcttgctt ttctcgcact
     8941 tactgtaact tttacatcag tgtctggatg aatgtaatta tctaacttac ttagtttagt
     9001 ttcgatttta ttttttattg catcagttaa ttcgatttgt tttccagata taattatgtt
     9061 cataaataca tctcccttca attatgtcag tataagaaaa tttatacttt aataatatat
     9121 tctacacgaa ctctcataat cctcttttta ttatttactt ttatgatttt ttctattcaa
     9181 ttgatagaat tatttatcat ttttagttac attgatactt atgtggatta atattattac
     9241 aaacacctgt ggataatgtg gataaaactg ttaataactc ttgtttttgt ttatttaaag
     9301 ctatttttac tcctatttct atccacaaaa atctccatta attgtttatt aatatgtgaa
     9361 ttcatgtcat aagatggtaa ttttaggaaa ttatgtgtat tatatgccaa tataccttta
     9421 taatttttta ataactataa accctctaaa acctccataa tctatctttt ctttcattat
     9481 ttatatttaa aactacttaa aagttataaa gcatactcaa caataatttc atatttaata
     9541 cctattaaca ttcatatacc caaattataa ttaacttact ctctttattt aaactaagta
     9601 ttttatattc atatactatc ttgaaattat atttacgagt tgtttaagct tttgtctatc
     9661 tatatcttca aaaggactgt tatttattct aatcgctgta ccaaaatgaa aattacttgc
     9721 tttagttaat tcctttatct tttctatatt attaaaatta agacctcctc ctaaaagaat
     9781 ttttatgtga ttagaactaa gcatcatatt ctttatcatt tgtatgttat caactatact
     9841 tcctttccct cctgatgtta atatattcgt aattttatca taatccttca aaattttaac
     9901 actatctatt gtatttgatt catctatggc tctatgaaat gtaacatcaa gattatcaca
     9961 acatttcaat aaaacattta gatttttctc atctatatta ttgtttttat ccaatacacc
    10021 aaatacaact ccattagcac caatctcttt aaccacacct atatcttttt gcattatact
    10081 tatatcttct ttactgtata caaaactttt cgcatgatgt cttatcatta cgttcacagg
    10141 tatctttaca ctgttaacaa cactttctat aagaccaaaa cttggtgtca atccaccttc
    10201 agttaaagca ctaactaact ctattctatc tgcaccacaa tcttctataa ttttagcgtc
    10261 ttctactgac attcctatta tttctaacat aactactcct ttgctaattc atatattgca
    10321 tttgcaaata tctttgcatt taatattata tcttctatat ccatgtattc atcaggatta
    10381 tgagctattc ctttttgtcc tggaaatgaa ggtccaaacg gaactatatt tggcattact
    10441 tttgcatacg tacctccatt agttgtcacg ggagttccat ctaatgatgt aaccctttca
    10501 taagctagct gaagcttttt aacaagatta ctctctttat caaaatatac aggattaaaa
    10561 ttagaaatta cctctatatc gatactattt ggaaaactag cttttatttc atctaataca
    10621 tctttcaatt tataactaaa atcgtatttt acctttatat ctaatatagt attactattt
    10681 tctatattca gctctttata tgtaacaagc acattttcag acatattttc tataaaacct
    10741 aggtatacat cttcttcata attgatttta ctttttattc taatctttgc catgcctttt
    10801 tcaccatata caacaggata tttacaatct ggtgtaaatc ccataatagg tgttttttct
    10861 ttttctaaat aatatggtat atcttcaaat ccactttctt catttgtacc aaatattatt
    10921 ctaactctct tatctaattt taagtttaat tctttaatag catataatcc atataaggca
    10981 gacattatag gtcctttatt gtctagtact cctcgtccat aaattcttcc attagtttct
    11041 tctcccttat aaggctgatg cttccaacca tctccttcat gtacaacatc tacatgtcca
    11101 attacacaaa cataatcatc actatctcca tgctctgcat aaccaatata attatccaag
    11161 tttctaacct taaatcccaa tttttttgat atttctaatg ctttattaag tgcctcccct
    11221 actttttcgc caaaagggaa gccattttct gactcccctt tgacactagg tatttttaca
    11281 atatctataa tatcacttaa taaatcttct tttaactcat ctattttatt acttatgata
    11341 ttcataaaaa tctccttatt ataaaactct attttaatta tgtaacacta ctttactcac
    11401 ttacttctat cggtgctttt attctagcct catatgcttc catccactca tttgatataa
    11461 ccttatgtat agtctttcca tctactctat ttgaaatata tacagcagga gattcttctt
    11521 tagttactac tgaaaaagtg aaatcaacta tatttgtagc tactccccat tcacctttat
    11581 tattcatggc aactaatgat aaatcaccag cttttcctct tcttttgata agctgtttat
    11641 caagttcatt tactgcaata tcacatgctt cttgtggatg ctttccttct ttcataagcc
    11701 taactatctc ataagatata catcccttca ttaaatcttc tcccaaacca gtagctgtag
    11761 ctcctcctat actactatca acatagaatc ctgaaccaga tagtgcagaa tctcctattc
    11821 tacctctttt cttcataaac aatccacttg ttgatgtagc tgcacacatt tttccagttt
    11881 tgtctaaaga tatcatacca acagtatcat gaccagcata tgggcttaat cctttgtcta
    11941 aagtctcttt ttttctcttt ttataatgta acttcgctct atcagtcaac atattttttc
    12001 tttcaaatcc atttttatga gcataatctt cagcacctat tccaactaaa aaattattta
    12061 ccttctcata acttaacttc ctagctatgc tgacaggatt agaataatct cttataccag
    12121 caactgcgcc aatatctaat gtgttaccat ccataaatgc agcgtcaagt tctacctcac
    12181 aattttcatt tggaagtcca ccatatccaa ccgatttata gaatggatag tcttcaactc
    12241 ttcttacagc aagctctaca gcatcacctg cttccatgcc tttttctaaa tgttctgatg
    12301 cctcagttat tccttctagg gccattctcc atgtcgctat cattccccac atgttaaaat
    12361 tcccctcctt aaattatata gattttacta atgtatgctt attataaata gtataatatt
    12421 taaacttgct aataattaat actcttatta ataacaaaat tcttctttca tcaaagttat
    12481 atcttcttta ttcgctattg cttttgccaa tttacatatg ttatttaatg aatcattaag
    12541 accaattcca ccttttttgg gagtcttaac tatacatatg tcatttttat attcgtttat
    12601 cacatctata ttttcttctg acatagctgc aaaagctggt atatctgttt tatcatttat
    12661 atttttagac agtattgctg acatcttaga aaagttttcg taattaaagc ttggtccaca
    12721 gattactaca tcagggttta atttttttac catagctatc atcttactcg ttacttcttt
    12781 ttcattattt acaaaaaatt catccccaca aaataaggta gctattacct tagattcatt
    12841 taaaaattgc tccatcatta cacctggtcc taatgggctt gattttccag caggaggtat
    12901 attacttttc tcttttcctc cagctccagc ttgtatttga tccaatatca taactatttt
    12961 cttcataatc ttcctcctat aatactaaat catcaaaaga tatatcatca tcttcctctt
    13021 caacagtttc attttcttct ctcaagtatt gcttatctag catttttata aatggtatat
    13081 acataaatat tcctagtata agaagcacta attgtaagac tgcaccttgc catcctgttg
    13141 ttaaaaaacc agagaatata attggagttg tccacggtaa ctgaactcca tttgtaagag
    13201 gcactaatcc tgctgacata cagaagtaag ctattattat atttattgtt ggaactattg
    13261 caaatggtat caatagtatt gggttaagca ttattggtag accaaatatc aatggctcat
    13321 ttataccaaa tattcctggt aaaattgata gttttcccat tgactttata cgtttacttt
    13381 tacatattaa taacatagct atgactaatg atagtgtact acctgctcca ccaaatgtcg
    13441 caaacatatc ttggaattga cctgttatta tgtttggtat ttttgaacca gcctgaaaag
    13501 cagctaagtt ttcagctgat agaattttta atattggatt atacacagcc ccaacaacac
    13561 tacttccatt tattccaaag aaccagaata aatgtaagaa tatataagcg attgccatag
    13621 cacctaaact atttcctaat tttaataatg gcatttgtaa aaacttaaat ataaattcat
    13681 gtatacttcc atatggtgta gcttcaaaag ctatcttaac taaaaagaaa actaccataa
    13741 ctattgcact tggtattaat gctgcaaatg attttgatac ggttggagga actccatctg
    13801 gcattttcaa tgtccatcct ttttttacaa ctgcagcaaa taattttaca gaggttattg
    13861 ctgttatcat acctacaaac attcccttag agcccatcca acctagtggt atacctgtta
    13921 ccatgtagac tgattctgtt ccttctggtg tatatggtat tacaaacggt gttactataa
    13981 aaaaggcaac tattgcaact gcagctgatg ctattggatc tacatctatt tgctttgcat
    14041 aagaatatcc aattcctaaa actgcaagta aagtcattac ttcaaatgtt gctacagttg
    14101 gttgttgcaa ttttacagcc caatctggac caaaaatttg acctacaaat tcattccatc
    14161 cttttatagg aaaatttgct actaataaaa ataatgaacc tattattaat aatggtgatg
    14221 atattaaaaa accatctcta attgcaatta ataatttatt tttacctatt ttttcagcca
    14281 atggcataag tattctttcc aatctattca tctatccacc ctcatttcct attatttttt
    14341 actataattc tacgcttgtt tagattttat tagctttata gcaagtttta atactttttc
    14401 cccattcata gttccataat caacttcatt gattatatct ataagttttc cttgagcttc
    14461 aaactttggt tttaactcct ttagcatgta tttaacttgt ggtcctaata atatacaatc
    14521 tggatttttt tcttcaacta ttttgtcaat ttctgctatc ggaaatgcct ctacttctat
    14581 tggtaaattg tgttcatctg ctaccttctg cattttatct gcaagtaaac ttgttgacat
    14641 accaaagcta caaaataaat atacttttct tttcattctt ttaaatcccc cttaattttt
    14701 ttattttaat ttttgaattt cttttcttaa cataatcatt tcttttataa ggtttctctc
    14761 actcatacaa gtcattaaat ggtcttgaga gtgaatcaat aaaatagata tatctgattt
    14821 atctccccca gcttcctttt gtataagttc agtttgtact ttatgtgctt ttaaaatttc
    14881 ttcatttgca ttttcaatta attcatctgc ttttttaaaa tcgttttcct ttgcatagtc
    14941 aagtgcttca taaagcatag attttgcatt tccagcatga attataatct ttaaaactat
    15001 ttcttccatt ttttcctcct aaattgcttc tctactttta gattatttat accttacaaa
    15061 catataatat cttataaaaa caaaaaaaca tgcacaactt tttccatcac atggaaaatt
    15121 ttgtgcttat ccatagttca ttatgaattt tatataagaa tatcttttac cttttcaatg
    15181 tcacatttac ttagaatatc tagcatattt tcatcctctg ttaaattaga tatttcttta
    15241 aataattctc taaatacttt tctatcatca tcttttttat atgctaataa aagtataatc
    15301 tttatatcct ttccatttct aatttttatt ggtgttttta accttgcaaa acataaaaca
    15361 gattgtttta tatatttcat gtctccatga gggaataata ttccttttcc aacattggtt
    15421 ggatatgact tttctcttgt tataatagat tgtaaatact ctttttctgc ataatgtttt
    15481 tcaattaata aattagttaa atatgttata gcttcataaa tatcatcatg ttctaactct
    15541 cttattaaat aatcagacat cattaatctg ttcataagtt tatatgaggt gtctttttct
    15601 tttattttgt ttgtaaactc tttaagttta ttctcatcct catcagtaaa taatggtgtt
    15661 gttataaaaa ttggtttttg gatattttca tactcttgaa atgcaattat aaaatctata
    15721 gtcttattaa agttatctct ttttaaatct tcctctctta atgtttcaat aatttttatg
    15781 ttatcaaatc tcttttctat ctttactttt aatagcatac ttactccaaa actaaatgga
    15841 catacaatac ttgccttttt atttgattcc cttttgctgt attttctttc tagagaggtt
    15901 tgaaagtgaa gtgtaagata accaatttca tcttccgtta tttgttttcc ttttaattca
    15961 atttctattg aacttgatat tactgtaaat aaaaaacaaa atttagtttt tatgttatct
    16021 aaaagtggat tttctaagta aacattgctt ttcatttgat gcattgtaac attcaaatga
    16081 catattaatt gttcataaag taatatatca ttattaaaat ttattcctga ttcttctgat
    16141 actaaattta taattttttt tgtataactt gctaattctt catttagata taaagatttc
    16201 ttaatattat tactatttat ttgtttgttc atacccaata ttaaagattc tataaacaat
    16261 ttttcttcat aatttaatac tatggataaa ttagatttca attcatcttc tatatccaat
    16321 ataagcattt ttagcttaag ctctttctta gtatttgata cttttaaaga atttcccagc
    16381 cttattcgta ttatatttat aagcaaaaat agcatcaatt gcttgaatga tatatctgta
    16441 agtcttatat ccatatcctc ctctatagta agcattatgt tttttacaat tattatatct
    16501 actatatgga agtactctat ttcatctatg tttaatctgt atttatctat gtatttaaat
    16561 aagaatgata ctaacattat tcttatgtct ttctcatccc cttcaatata tataccactg
    16621 ccttttttag tcgagacatt taaattataa ttatttaatg ctactgataa tttacttatt
    16681 tcttctctga caatattttt atttatgtac aacttattag ataaatcatt taaagtaact
    16741 tttttattat tacaaagtag taaatttaat atctcaactt gcttatgaag ctcactattt
    16801 actcttttaa cttcacctcg caacttctga tttaccagta ttttgtctgt tccatctcca
    16861 ctaaatttaa ttccaatatt aggttttctt ttgatataag cattacaaaa agtagataac
    16921 cattcatcaa tatatttgca atcattcctt attgtccttt ctgaacactc aaaatcattg
    16981 gcaatcttat taacatttat aaactcttct tttaatagca atgttgttaa taaattttct
    17041 tgtcttttat tcaaacatat cacctcttta tgactcaacc taaatttatt atatataaaa
    17101 aagagctata cataaaggta tgctagctct cttttaatat gatatttagc tgacctggtt
    17161 tttgacccca cactcgccta ctggagggtt cgcttgggtt cgccgcacta ctactctctc
    17221 tcgattttat tatcggtagg tcttacttct aagccgcacc atgatcatta acccaatttt
    17281 tagtgttaac ttacgtggat ccctttgcct gcgactggct tggactttca accacagacc
    17341 taaatatcac ttattcattt taatatttta aactaaaatt gtcaatatat aagattattc
    17401 cataatataa ttatcatttg cttttgtaag tagagtcaat acaaaaacct tattagcacc
    17461 tgataacttt aaaagtttag agatttcgtt tgttgttaga ccagtagtaa atatgtcatc
    17521 tattaaaagt atatttttat tatttattaa tttaacattt tctttaacaa caaatacatt
    17581 ttttaattct tcttttcgtt ctttcttatt caatttatat agcattcttg tatatttttt
    17641 tctacttatg caatctaata atggaatatt tactatttta cttaaattaa atgctatttt
    17701 ttgtgcctga ttaaaacctc ttttatttag ccttttttta tgaagtggaa caaataatat
    17761 ataatcaaat tttatgtttt ctaaatataa tttttctttc attatttgag ctatatactt
    17821 agccataaag gttttttggt tatacttcag ccctaacacc atcttttttg tcacatcatt
    17881 atattcaata caagatattg atttatcaaa ataaaagtct ttattaaaac aataactaca
    17941 ctcttcaata aattcttttt caattgaatg acgtattatt ggttttccac acttcatgca
    18001 accatcttgt ataaaattca tttctttaaa acaactttta catattgaat aagtatttgt
    18061 tttctttatg gatttatcac aaattatgca acttatattc tcaggatata taaaatccaa
    18121 acatttatta atcatatttt taaaattctt aataaaatta tcattgcata ttttcatatt
    18181 tattctatca acaacccttc ttgtttaaat ttatttagct tatatcctaa atttgaatac
    18241 ctttgattaa ctctattatt ttttatcata tattcaagat atttcacatc tccaactaat
    18301 acaactaatt tttttgctct tgttactgct gtatataata aattacggct aagtaacata
    18361 ggaggagccc acgctattgg caatactaca actggaaatt cacttccctg gcttttatgt
    18421 attgttgtac aaaaactatg gtcaatttca tctaattcat catataggta tgatacaatt
    18481 ttagtctggt caaataatat ataaatagtt ttcttatctt tatctatgtg ataaacatat
    18541 cctatatctc cattatagat accttctcca ctttcttttt ggtcttctgt ttcccatttt
    18601 tttgtataat tattttttac ttgcattacc ttatcgccaa ctctaaataa tcttttagaa
    18661 aaactttctt caactttaaa tttttctttt ttgttaagat acttctgtaa ctctatattg
    18721 agattagtaa ctcccaattc accttttctc attgaagata aaacttgtat atcttttaat
    18781 ttatcaactt tataaaattt aggtaatctt tcattaacca atcctattat ttcatttaaa
    18841 atttcttcat tagttgattt tcttataaaa aagaaatctt taccttttgt atttagataa
    18901 agtggttctc cattgtttat cttatgggca tttacaatta tcatactttc ttgcgcttgt
    18961 ctaaatattt catttaattt aactacattt ataatattag aattaatcat atccttaagt
    19021 acatttccag cacctacaga aggtaattgg tcactatctc caactaaaat tactcttgtt
    19081 cctaacttta tagctctaag taaattgtac attaagataa tgtctaccat tgatacctca
    19141 tcaacaatta ttacatctga atttattggg tcttcttcat ccttaaaaaa agttaaatca
    19201 tcatctgttg aaaatcccat ttctagcaac ctatgtatag tttttgcttc tttattagaa
    19261 gtttcactca ttctctttgc tgctcttccc gttggagctg ccaaagtcac acttttacca
    19321 ttattttcaa atatatctat gatagcatta atggtagttg ttttaccagt tcctggtcca
    19381 ccagttataa ttaatactcc actattaaca gattctttga ctgctaaaat ttgtttttct
    19441 gctaacttaa ttttcttatc ttcttctaaa acattaatct cgttatctat atctattttt
    19501 aagtcttcaa attcatactg tgataatttt actatttgac tacatactcc attttcagct
    19561 aaatagtatg gcataaggta tataagattt tcattgttta ctttctctat atgtattttt
    19621 tgattataaa ctagcatcat tacacactct tttattattt ctccatttag ctccaataat
    19681 ttttccgaat cttgtattag aacatgctct ggtaaatatg tatgcccatt acttaaagat
    19741 ttatttaaag tgtataagat tccctgacaa attctatctt tagagttttt atctatacca
    19801 attttacttg ctatactatc agctacttta aatccaattc ctcttatatc ttcagcaagt
    19861 tggtatgggt ttttatttat aacttctatt gcactgcttt tatatttttt atatattttt
    19921 aggcagtaat ttggtgttat tccaaaagga gatagctgta ttattatatt tctcaattct
    19981 ctgtcttctt cataactttt tactatttgt tttacttttt tactccctat accttctact
    20041 tcttgtaatt tttctggtga attttgtatc acctctaacg tatctacacc aaacttatca
    20101 attattcttt ttgccatttt ttcaccaata ccatgtatca ttccagaaga taagtaaaca
    20161 taaattcctt ctaaggatga tggcgttact ggcataaaac tattcacttc aaattgagtc
    20221 ccataaatct tatgatttac ccactttcct tcaacttcta tgctttctcc aattgccaaa
    20281 gtaggcatac atcctacaac cacaatttca tcattttcat ttaccaggtg agctattgta
    20341 taaccattat cttcgttttt aaagactatt tcacttatca ttccttctaa tttttccatt
    20401 tttatctcct aaaaatacta ataatcgctc catttaaatg ttagtataat tttatatatt
    20461 aaaactatga taatcgtaga aaagttttat atttttatac ttaacgtaat ttccaactat
    20521 tatacatttt atcagaaaca aaaaaaatct gcatccataa aatatggtac catgaaatac
    20581 aggtaccata tttaaatagc tattttattt taattactct atatttagag tataaattaa
    20641 attccataag tatattacag tactaactta tttcagattt attctccttg tacccctcag
    20701 ttttagtaat tcttctataa agcatacata gtaaaattaa aattcccatg taacctgtat
    20761 atggatatag tgtacctact aacttatcaa atggcagtaa acctcctata caagctaata
    20821 tacttaccac tattgttata attttaaatt tagggtggtc atcttctaca aatcggttag
    20881 ttactgacca taacaatggt acagctgtag tgtatattcc tagtaaaagt actactgaga
    20941 ataaaatacc tattattgga gatatcttat cagctaagta aagtgctggt atctcttttg
    21001 tgtatatatt tcctatgttt gaaagtaatg ccaaattcat cattatagct gctgccataa
    21061 gtgctactcc acctactata ccaccccata cagcatcttt cttatttact gcagaagctc
    21121 ccattcctgt caagaatgtt attactatta ttacattgta acaagtataa agtactcccg
    21181 agaaatatcc atttgatgta gcagttttaa tatttaaact atcaaccata tttccagcat
    21241 ttgataaagc atcaatattg ctaaataaac taattgctcc tactaaaagt gtaaatatta
    21301 ttattatagg tcctatatta cctagtattt tagaaagcct tgtcaaacct aatgaaacag
    21361 ttattaggga aacgattgcc ataccaatcc tacctacata tggatttaat ccatagtact
    21421 ctgatagtgt agctcctgct cctgcaatca ttattactaa tgttccaaat aaaaacaatg
    21481 gaccaaacca ttcaaaaaat gttccaagat attttccaca atatacttga taaattttta
    21541 ttggctcctt aagtttcaat tctcttccct tatctataac ttctgctcca aaccatgaaa
    21601 atagtaccat agagattatt ccacctataa tacccgcata tccataaaac gtaaaaaatt
    21661 gcagtatttc ttgaccagtt gcaaatccag aacctattac agtagcaaca taagctccag
    21721 caaaacttat aaccgtttta atatttactt tgcctgacat acactactcc cccccaatta
    21781 aaattatttt taaagtgtaa gtttattaag attatattag actcattatt gtgatatttc
    21841 aatatatttt taattattta acattagtta acacattctt aaccatcctt taacatatat
    21901 cccataatat gtataaattt agtagagtat tatataaaat gtattgacca gattaaatac
    21961 tgtttattca ttatatgatg ataaattaac atttattaaa aatatttaac tcataataaa
    22021 aaaactgtta gaaagtaatc gtaaaattac attctaacag ctttttagtt aaaaattatt
    22081 cacctaaagc atcttttctt aactgctcaa ccttgtcagt tctttcccaa ggtaaatcta
    22141 tatcagttct accaaagtga ccataagcag caaccttttt ataaagaggc tttcttaaat
    22201 ctaagtctct tattatcgct cccggtctta aatcaaagtg tttatttact aattcaacaa
    22261 gtttttcttc tgaaaccttg cctgtcccaa aagtatctat aaatatagat aatggtctag
    22321 ctatacctat agcatatgca agttctatct cacatttgtc tgcaagacca gccgcaacaa
    22381 tattttttgc aacatatcta gctgcataag cagcagatct atcaactttt gtagggtctt
    22441 ttcctgagaa agctcctcca ccatgtctag aatatccacc ataagtatct attattattt
    22501 ttcttcctgt aagacctgta tctccttgtg gtcctcctat aacgaatctt cctgttggat
    22561 ttatataaat tttagtctct tcatcaagta attctgctgg tataacttcc ttaataacat
    22621 gattaattaa atcttctctt attttatcat ttgatactgt ttcacaatgt tgagctgata
    22681 taagaactgt atgtactctt acagctttac ttccttcata ttcaacagta acttgagttt
    22741 ttccatctgg tcttaaataa tcaactaaac ctgtttttct aacttctgta agccttcttg
    22801 ataatttatg tgccaaagat atcggaagtg gcattaattc tggagtctca ttacatgcaa
    22861 atccaaacat tataccttgg tctccagctc caactttttc tatttcttct tctatctctt
    22921 ctccagtctt actttctaga ccttcatcaa ctcccatagc tatatctcca gattgttcat
    22981 caatagaagt tataactgcg caagtatcac aatcaaatcc atattttgct cttgtatatc
    23041 caatttctct tactgtttct cttactaatt taggaatgtc tacataagca gatgtactta
    23101 tttctcctgc tactaaaact aaaccagtag taactgtagt ttcacaagct actctagatt
    23161 gtgggtcttt ttctaataat gcatctaata ttgaatctga tatttggtca catatcttat
    23221 caggatgtcc ttctgtaact gattctgacg taaataaatg tcttgccatt tttattcccc
    23281 cttatttatt cttaattttt attataggtt ttaccttatt tttaatttta gaaatttact
    23341 caaaataaaa aaacctctcc tttataagaa gaggttaata atcgtaatta catactaatt
    23401 aactgacctc atctttcaag acttttgtct tgtaggaatt agcacctttt cccatttggg
    23461 tggttgctgg gcttcacagg gcctatccct cagcctctct tgataaggta atcaagattt
    23521 aagttttgtt ttactttact agcttactat ttttttaaaa ttttgtcaat atttttagtc
    23581 atattttttc tattcctttt tttgtaagac aaataaaatt ttctatctat ctgaaattta
    23641 aagttaggtc tttttaatgc tttttattca tatatttttt tgaagggggt gttcctattg
    23701 tatttagcta aagtaaacta cagcgatgat gatgttatac agatgctaag ttcagttcta
    23761 aaaactataa ttaatgaaaa tacgattgtt gtttgcatag gaacagacag ggctataggt
    23821 gatactttag gccctttagt tggaacaata cttaagaaca gtaactttaa ataccccgtt
    23881 tacggtacac tagataaccc tattcacgcc ttaaatatat atgagtcttt agatactata
    23941 aaaaatacac atatacaagg taacttcttg gctatagatg cttgcttggg gtcgcagagt
    24001 aacataggta atatacagat tagagaagga cctatcctgc ccggaaaagg tgttggtaaa
    24061 aaactacctc aaataggaaa ttattctata gtaggaattg tagataaaat tgatgaaaat
    24121 aataaacttt catttaataa tatacgcctt agctttatat tagatttagc tgaaacaata
    24181 gccttggctt tattagtatc cacctaatct aaatataaac taaatgataa aattctttat
    24241 ataaaaaggt agatatttta gagtatctga ttaaatactc caaattatct acctttttac
    24301 ttttatattt caagttttta tttttctact aaagcaaaca ttaattcacc tttacagaca
    24361 agttcttcac ctacatatgc cttagcatct gccttagcta tattttttct aagagttgtc
    24421 atttcaattt ccatagttaa tgtatctcct ggtcttactt ctctcttgat tctaacttta
    24481 tctatacctg caaatacacc tagttttcct ttgttttcct ctacagacat cattgccact
    24541 ccacatactt gtgctagagc ttctactatt agtactcctg gcatcaaagg atattctgga
    24601 aagtgacctt ggaaaaatgg ttcattaact gttacattct ttataccaac agctcttttc
    24661 ccaacttcta gctctgtaat cttgtctact aataaaaatg gatatctatg tggtattaat
    24721 ttttttattt catctatatt taacattcta aactctccta attattttct tttgaaagac
    24781 ccaccagttt caagtaagtc tattgaacct aaagctattc ctgtaccttt tgcaacacaa
    24841 gataatggtt catctgctat tgtaacagtt attcctgtcc tctgttctat tattttatct
    24901 aatccatata ataatgaacc tcctccagtc attattatac cgacatctcc tatatctgct
    24961 gctaactcag gaggcgtttt ttccaaaaca gcatgagtag caactactat ttgctcaaca
    25021 cattctctaa gtgcttccaa catttctgtt gaatttatag ttatagaact aggaagtcct
    25081 gtaattaaat ttcttcctgt tatcttcata tatttttctt cttctctctt aaatgcagaa
    25141 cctatttcaa acttgatttt ctctgctgtt ctttctccta taagaagatt gtgttgtttt
    25201 ttcatatatt taactatagc atcatcaaat ctatctccac caacctttat agattcactt
    25261 acaacagctc ctcctagaga tattacagct atatctacag tacctccacc aatatctata
    25321 atcatattac cattaggctg tgatatgtct acaccagctc ctatggcagc tgctattggt
    25381 tcttctatga tatatacttc tctagctcca gcttgtctcg tagcatcttc tactgctctt
    25441 ttttccactt ctgtgacacc tgttggtaca cataccataa ttcttggcat aaaaaatctt
    25501 ccaaatccct ttttatcaac tatcttatct ataaaagatt ttaacatctt ctcagttaca
    25561 tcataatctg aaattacacc atctcttaaa ggtcttatag cgactatatt acctggtgtt
    25621 ctccctatca tctttttagc ttcttctccc acagctaaaa cagaatctgt tcttttgtct
    25681 atcgccacaa cagatggttc ttctaaaacg attcccttac cactaacata aacaagcaca
    25741 tttgcagtac ctaaatctat gcctatatca gctccagcca ttttttcaac tcctttattt
    25801 ataatattag attatattat aagcctattt taatactcct ataataaagc atatacaaaa
    25861 atctcgcttt aatcaagaga aaataaatct tttgagagta ttattatttt gctatttata
    25921 attacttaat atatttcttt ttataattta attcaataaa aaaagtaggt ttttaaccta
    25981 cattttttta tgttccttct catattttaa tttcgttgct aatccccctc taatatgtct
    26041 ctctgattta tttttgtcca gcacattttt aacttctttg gcaagggatg gatttatttc
    26101 cttcaatctt tctgtaacat ccttatgaat tgtactttta cttactccaa atgtcttggc
    26161 ggtttgtctt actgtagtgt ttttttcaag tatatatttt gctaccacta tggccctttc
    26221 ctctatatga gatctcaaag attacccccc tcacataata gctttaatga tatatatgta
    26281 aaaacaccaa aaaatataac aagcttaacc aaatatgatt aaacttgtta tacttttact
    26341 taattagact cattgggtct atagattttt ctccactata cgcctctaaa tgaacatgta
    26401 taccttcttc actttctatt tgagaagtac tacctacagt acctagagat tgtccttctg
    26461 ttaccttctg ttctttctta acactaacat ttttatctag atttgaatat actactacaa
    26521 tgttattgtc actttttatt ttgactgatt gcccatattc ttcatcatta aatacatcta
    26581 ctactattcc gtttaataga gattttactt ctttgccttt agtacaacta atatctacac
    26641 ctttatgagt ttcccaaaca tcaagagttt tagaataact aggttctttt tctgaatatc
    26701 ctcttattac tttatctcca atgtaattta attttgttgt actcgattta ttttcctctt
    26761 ttgacttagc tttttctaag ttttgttttg aatccgttgc agtagggata gcatctttct
    26821 tgtctttttc aattaaatgt atttcctctt cactatcttt attagcattt tccataattc
    26881 ctttattaga agccaattta tctacattat tatttgtaaa ccaaactcca cctactgcta
    26941 ataaacaaac acatacaaac aaagataaat aaaaaccatc tttttctaac agctttttct
    27001 tcattttgca cctccaaata tatttctaca tttggattat tgacgtgttt ttggcatttt
    27061 atacaattat tagtatatat cttttatttt tgtatctgtg tagtaatgtg ataatatatc
    27121 ataatattta tagccctctt ctgccattcc ttctgctccc cactggctca taccaacacc
    27181 atgaccatat ccttttacca cgaaatctat gtatccttct ccaaatttta tgtcaaagtt
    27241 tgcagagttc aatttaaaaa cagttctaat gtcttttcca gtcaattctt tatttcctaa
    27301 ttttattttt tctacagttc cggcatcact tcttttagtt attgaaactt gtttacttaa
    27361 attattcaca tctatcacaa tagtgctata tgcccttcta agacttttaa caaaatctgt
    27421 gttacttatt ttaagtgttg acgcaaattt aggggaatac ttatcataag gactttccac
    27481 agactttaaa taaggatatt ttgcagaaaa tacttcttca ctattttctg tttttcctga
    27541 agatgtagaa aaataaagag gaagtatcgc cttatcatta taagttatta tttgaccttt
    27601 agttcctcta actgcttctt ggattttgga atatttattc ttaatccatt cttcaccatt
    27661 taatttttta agagtatcat aactcttata ttcttgacaa tgtttataat cagtacatac
    27721 tactgcattt ttatgtttac tagatttacc atgttcctgt ttatatacta catatgttct
    27781 agctgctaca gattgagctt ttaaagcttc tatatcaaac tctgaagaca tctcaccagc
    27841 tagtactcca cataaatagt tctccatgtc catcttttcg gttttccctg ttatatgatt
    27901 atatacattg ataattggtg cttttttatt tacagtttca tagctcactg tacttttttc
    27961 ttctttatta cctttgtctt caatatcact ttttttaata gttttgttta tagatactgg
    28021 gctctctggt ttttcagtta attctacatt tttataagaa actaaagtta taagtgatgg
    28081 aactaacaca gagcaagtca caaatcctaa caaaacaacc aatgggttct tcatatactc
    28141 tcctccttag cttttaaaac cttgtattta agtatatgaa caaccctatt aatttataac
    28201 tttcttctat aaaccaatta ttaatcttct actatttcga tttgaccacc tagtgctgtt
    28261 atcttcttat ctatatcaac ataacctctt tggatatggt atatttctcc tatttgagtt
    28321 tctccttctg ctataagacc gcataaaata agggctgccc ccgctcttaa atcagtagcg
    28381 tttactgctg caccgtgtag ctcatctaca ccattaacaa ccgcacttct tccatctatt
    28441 tttatattag cacccattct attaaattca gcaacatgca taaatctatt ttcaaaaaca
    28501 gtttctataa ctacaccagt tccattggct acagtaagca tagccataaa ttgtgcttga
    28561 acatctgtag gaaatcctgg atgtggtaaa gtttttatat ctattggttt taatactttt
    28621 ggacctacaa ctcttacaga attgtccatt tctgttattt cgcaaccagc ttctcttaat
    28681 ttagctacaa ctggttttaa atgctccatt aaaacatttt caacagttat atcacctttt
    28741 gtcatagctg ctgcaaccat ataagttgct gcttctattc tgtctggaat tacattatgt
    28801 tctgctcctt ttaactcttt aacacctttt atttttatag tattagtacc tgcaccttta
    28861 acatctgctc ccatttcgtt caagaagttt gctaaatcta ctatttctgg ctcttctgct
    28921 gcattctcta ttattgtagt cccttctgct aaagaagctg ccatcattat gttttcagtt
    28981 gctccaacag atgggaaatc taaatataat ttatttccta ctaacttttc tgttgctgct
    29041 tcaacaaaac catggtccat ttctatcttt gcacctaagg ctttaaatcc ttttaaatgt
    29101 aaatctatag gtcttgttcc tatagcacat cctcctggca tagaaatttt cgtactatta
    29161 aatctagcaa gcaatggtcc cataaccaaa aaagaagctc tcatctttct tacaagctca
    29221 tatggagctt cacaagtttt tatattgctt gcatcaacag taagtgtatt ttctttatat
    29281 tcaacttctg ctcctacatg tcttaataaa tctgatataa catgaacatc tcttaaattt
    29341 ggtactccat ttaatgttga ttttccgttt gctaataaag tcgctgctat tattggtaaa
    29401 actgcatttt ttgcaccatc tattttaaca ctacctttaa gtggattact cttttttact
    29461 attatcttag ccatattttt tgatctcctc ataattaata atctaatttt ataattggtg
    29521 tggcaatata tatgagtgtt tttccgtcat cctcattata tttaattcct atatttaaat
    29581 taattttttc ccctaaatat tctaattcat aatcatttaa tactttacta tatgctgtta
    29641 tagataaaaa gtttacgtct ttaacttcat ctatttgctt agcactcata ttatataata
    29701 ttttttgtaa aatatcatca tatttgttgt tttgtaacct ttttgtgtat tctccaacaa
    29761 tacaagcaga cacatctact tgagatgaat atttatttaa cacattttct acgttactat
    29821 aaataacctc tttatgttta tatactttgt tatccaatat gtcaactact atgtaacttt
    29881 ctttgttatt tttatttatt ccttttatag aaatttcttt atcgctacct tttatagtag
    29941 cacataattg tattttatcg ccagaccctt tttgattcca atttattcct tttttatcca
    30001 actttagatt gttaattatt tctataaaaa tattatgaaa ttctttattt gataaattat
    30061 gatttataac agaattaatt ttaacattat aaaatttaaa atttgcttct gatatttcaa
    30121 gagctttact taatctattg tatctagtat ttatacttgt atctgcataa gatattgtca
    30181 ttcctataat cattaatata aaaaagctta tagctaattt tacttttttc atagtaaaaa
    30241 atccccctta aatatgtttt tctgtataat agaattatta acaaatttaa gagagatata
    30301 ctttctcaat atgtattttt atacattttt gtatttttca tctaataatc tctttcaaac
    30361 tcttcaatct caattttctc tatccttctc ctggacttgc caattcctta tatgtatcaa
    30421 atacatcttg cattaattct tatatattat cttctgtaat ataaattact tagtctatac
    30481 ttaaatctat aatctataat ctataatcta taatctataa tctataatct ataatctata
    30541 atctataatc tattcatatt ttaccagcaa aataaacaga taatactttt ttatcaattt
    30601 ccatagctcc aaatgaacat acctaagtac acttcccaat acacaattta cacactcttc
    30661 ctaataaatc aaaaacacct ttgttttaaa caactctatt gtactttcaa aaactttatt
    30721 tgtgttctcc cacttcaatt aaatttgaat tatcaagact ccagcccatc catccatcac
    30781 tataaaggga ggtattttcc actcctatga catttgcata tgttaatact tcagcagcac
    30841 gccatccact tccacacata aatataagct gtttattcac atctatattt tccttgtccc
    30901 acatttctaa aatctcagat ttatttctca ttgtattatc aatattacga tattcttcta
    30961 gtgaaacact attactacct gaatgtccat ataaagctcc tggtatacgc ccttttttat
    31021 cataatatgt atatccagat acttttccta tatgttcatc ccaagtacga ttatctacta
    31081 gtataaactt atttttttct tttagttttt gtcttaattc agatgtagta acaactaatt
    31141 gtgagttaac tggtatatct gctccaaaat tagtacatga atgttttgga ttactaataa
    31201 attctaattc atatcctgct gataaccatg agtttgttcc accattcaaa actcttacat
    31261 cttttactcc tatatatctc aatataacag ctagtctata agatgccata ggtgtaatac
    31321 ttgaaactat tacagtatca tcctttgtta gtccatagtc taaagcaaac ttagttaagt
    31381 tatcatcatt atcaagcatc catgttggag gaggttctat aatatcagta tttacatgaa
    31441 cacttgtagg tatatgccct tttgtatacg attcttctcc ccaactcgct tcaatcattt
    31501 ttatattctt tgaatcttca aatgtctcag gaatttttcc atctaatata tcttttatta
    31561 taaaagcagg aactatcatt tgataatttt tatatctttc cattggaaga ttttcatcgt
    31621 ctgcccattg tttaatatca tatttatata gatatttata tcctttttta gataaataat
    31681 ctgctacaac caaagcatct tttccatttg catcatacaa tactatattt ttatctaaat
    31741 caattctttt agttttcaat gcttgttcta gcacttcatc ttttctatct gagtatacat
    31801 ttaaccaatt agcagaaaaa tctactgctc tttttatatg cccacctctt tttactccat
    31861 ctaacttcca gccattataa gcatcattta agcgagtatc aactataacc caagcgttat
    31921 cttgcatttt ttcttgtaaa acttcagtag atattgcctt aagattaaca aaataatctt
    31981 ctttcttttc ttttttaaaa tcatttgtca taatttatac tcccaattta aaaaaatttt
    32041 tatttgaaat tattttacta aaataaagta attttcttat actctattag cttcattata
    32101 ttcttttata aatagttcct tatattctta ttttaaacta ctcactttat cttatcacaa
    32161 ggttactaat accttgtatt ttaatttttc tcataagcta ttattttttt atgtctgata
    32221 ttgactatat gtttattaag tactcactat attttatatg aactatattt atcgttccct
    32281 atacctggta tggtctatat atttatcatt ttacagatga tttaatatca atctttttat
    32341 aaaatttatt atagcaaaaa caaaacaaat ttaatattta tctaaaaaac tcatcctata
    32401 aaatacaaat aacaggatga gtttttttca ataaatatta tctatttaat ttttaatctg
    32461 tttttattca actgttactg atttagctag atttcttggc ttatctacat cgcatcctct
    32521 tagtacagag acatgatacg caagtaattg cattggtaca actgctaata ttggtgataa
    32581 aatatcatct gaatttggta tatagattat tttatcagca gctttctcaa catccttatt
    32641 atggctttga gctattgcta ctacataagc tcctcttgct cttacttctt ccatattaga
    32701 aaccattttt tcaaataact tttcttgagt agctatggca attacaggag ttcctttttc
    32761 tattaaagct attgttccat gctttagctc tcctgctgca aaagcttctg catgtatata
    32821 tgaaatctct tttaatttca atgaaccttc cattgctaag ctatagtcta tacctcttcc
    32881 taaatagaat gcatgttcag aactaacaac tgttttagca acttctttta tatattcttc
    32941 attgtctaat atttcttgta tttttgatgg tatttctttc atcttagaaa tcatactatc
    33001 ataaaattct ctagttattg ttcctttttt gatagcaaag tctaaagcta tcatgtataa
    33061 agaagttatt tgagtagtat atgccttagt tgatgcaact gctacttcag gtcctgccca
    33121 agtatagaat acatcatctg attctcttgc tatagatgaa cctacaacgt tagttattga
    33181 aagtattctt gctcctttag cctttgaatc tcttaatact gctagagtat ctgctgtctc
    33241 accagattgg ctaactaata ttactaatga attttcatct acaaatgggt cgctatatct
    33301 aaattctgat gctatatcag ttattactgg tatatttaca aatttttcta tggcatactt
    33361 accaagtaat ccagcattat atgctgttcc acaagccact atataaactt tatttatttt
    33421 ttctaagtct tctttagaaa tatttatact gtctagaatt atatttccat tatcatcaag
    33481 tcttctttct aaagtttctc taactcctgt tggttgctca tgaatttctt ttgacataaa
    33541 gtagtcatat ccacctttag acgcagcttc tacatcccaa gttacatgaa atacttcttt
    33601 ttcaacaaga tttctattag agtcataaac tgttacattt tcgtctttta gatgaactac
    33661 ttctccattt tctaagaaat aaacatctct agtatatttt aataatgcag gtatatctga
    33721 tgctataaag ttttcacctt caccaacacc aactacaaga ggactatctt ttcttactgc
    33781 aactaattca tttccatgtt ctttacatat aacacctaat gcataagctc ctcttaattt
    33841 tgatatagtt ttgtatacag catctaataa attaccctca tagtatttat ctactaaatg
    33901 tgctattacc tctgtatctg tttgagattc aaacttaaca ccttcagaaa ttaattcttc
    33961 ttttatttcc atatagtttt ctattatccc attatgaaca actgctattg ttttggcttg
    34021 attaaaatga ggatgtgagt ttacatcaga tggttctcca tgagttgccc atctcgtatg
    34081 ccctatacct aaatttccat caataggatt cttttctaag tcttctgcaa gtacagaaag
    34141 tctaccttta aactttctaa tattaagttc ttcttcatta gaactattaa ctgctactcc
    34201 tgctgagtca taacctctgt actctagctt agaaagccct tctactataa cttctgctgc
    34261 ctttctactt cctaaatatc caactattcc acacattatt tgtgtcctcc taaaaatatt
    34321 atttatatat tttaagagtt aggtcatata agtttacttt ggcttccctt tgtccatagg
    34381 attgctccta ttatttaaag aagtctttcg atggtaaaca caccgcagag catccgccga
    34441 caattcgatt aactctgacc tcgtcaactt agttcctata ttactcaata attatgtcta
    34501 taactaagtt ctggcgctta gaaaggtgtc ctcagtattt gtatctgaga atacccttta
    34561 aattacgata atttttcttg tataagatta gctaagtttg ttgctaattc ttttatttga
    34621 ccttcctctt taccctctaa cataactcta actaatggct cagttcctga tggtcttatc
    34681 aaaactctac catttccgtt taaaatagat tctattcttt ctatctcagt ttttatttct
    34741 ggatattcca tatatttgtt cttattttca ttttttattg ttgcatttac taatacttgt
    34801 gggtattggc tcattattga tgcaatttct gataaaggtt tcttttcttg caagattata
    34861 tttgcaagta ttagagaact aagcactcca tctccagtag tgttgtaatc taaaaatatc
    34921 atatgtccag attgctctcc accaagatta tatccatttt taaccatatc ttctaaaaca
    34981 tatctgtctc caactgctgt agtagataaa tttattccat tttccttagc agctattgtc
    35041 aaccctatat tactcataac tgtaactacc aaagtatctt gcgccaattt attatttttc
    35101 tttaaattta aggcactcaa tatcattata tggtctccat caacaatttg acccttttca
    35161 tttactgcaa ttaatctatc agcatctcca tcatatgcaa gacctaagtc agcattatgt
    35221 tccaaaacag ccctttgaag ttgttctgga tgtgtagagc cacatttata gtttatatta
    35281 tttccatctg gtgaactatt tattgaaatt acacttgctc ctagttcatc aaacactatt
    35341 ggtgccactt tatatgacgc accattagca cagtccaaaa ctacttttaa tcccttaaag
    35401 tcagtgctta ttattgattt taggtagtct acatagtctc tttgtgcaca atgttcatgt
    35461 aattttcttc ctacattttc accaattgga agacaatcaa ttttgtctat atcgtctata
    35521 tattcttcta ttttcaattc aatttcatca tctaatttat atccattctt attaaagaat
    35581 ttgattccat tatattcaac aggattgtga gatgcagata taaccacacc acaatcagca
    35641 ccatattttc tagttaaata tgccactgca ggtgttggta ttattccaac agtaataaca
    35701 tcacatccaa cagacataag tcctgctatt aaagatgctt ccaacatatc acctgatatt
    35761 ctagtatcct tacctactac tacttttact ctgtggtctc cttgagctaa gacaaatcca
    35821 ccagctctac caagtttata tgctaaatca catgttagtt ctgtatttgc tactcctcta
    35881 actccatctg ttccaaaata ctttctcact aaagattctc ctttcataat tgaaaagctt
    35941 gtaatttgta ttatattttt atagttcata aaatatcatt ataggcttaa tttcagtcat
    36001 aaaaacattt taatacttat cagcaaaaaa gacaatagaa ataattctat tgtctttttt
    36061 ttgacatctt tttctttatt agttagttgc tttagaacat gtagcatcca tacctttttg
    36121 taaagcttct ctgtttaatg gaacgaattt ttcttttcta ggtccgaaaa cttttttgaa
    36181 tgcttctagt acagattcta tatctacagt atcagatact tttaagtaag cgcctaacat
    36241 tatcatatta gcaactttat catttcctaa ttcagctgct aattcatttg ctggtatata
    36301 ataaacatct acatctgttc tatcaacttt ttcttttatt aaagaactat ttactattat
    36361 ttttccacca ggaataacat catctttgaa tttttcaaat gctggtatat ttaataatat
    36421 agctgcagtt gcatcatctg ttataagtgg agaaccaact ggctcattag atacagttac
    36481 tgcacagttt gctgtacctc cacgcatttc tggtccataa gatggtaacc aagaaacttc
    36541 ttttccttct agcattcctg cataagtaag taattgtccc atagacataa caccttggcc
    36601 tccgaagcct gcacatatta ctctagctgt agacatttac ttcacctcct caacttcaac
    36661 atttttgaaa tttcctaatg gatagtatgg tatcatgttg tctcttaacc attgtaaagc
    36721 atcgtttgga gctagtcccc agtttgtagg acaagtagat aatacttcta ctattccaaa
    36781 tcctaatcct gcttgttgaa cttggaatgc tttctttata gcttttttag cttgtctaac
    36841 atgagctgga gtatcaactg ctactctttc tacaaataca gctcctgtta atgtagctaa
    36901 catttcactt actcttattg gataaccttg agtttcagca tttcttcctg attgagcagt
    36961 agtagctctt tgtccaacta atgtagttgg agccatttgt ccaccagtca taccataagt
    37021 tgtattgttt acaaatatag tagttatttt ttctcctcta gcagctgcat gaactatttc
    37081 agcagtacca attgaagcta agtctccatc accttgataa gtgaatactg tattttcagg
    37141 atgaactctt tttattcctg tagcagcagc tggcgctcta ccatgagcag cttcttgagt
    37201 gtcacagtta aaatatttat atcctaaaac tgaacatcca actggaacaa cacctacagc
    37261 atcacctaat actcctagtt cttctaatac ttcaccaact aatctatgga ttataccatg
    37321 tgtacatcct ggacagtagt gagtttgagt atcttgtaat ccttcagttt tcttaaatac
    37381 aacagccatt atctagcacc tcctgcaacc aattctccgg caataatttt tttagctttt
    37441 tcaactattt ctgctggtgt tggagtcatt cctcctggtc ttccatggaa gtaaactggt
    37501 aatttacctt ctactgccat tttaacatct tccaccattt gtcccatact catttcaaca
    37561 gtcaatatgt ttctagcatt aggaatttgg ttgaatgctt cgaatgggaa tggccataat
    37621 actttaggtc ttataagacc tgcttttatt ccttcttctc ttaatatatc aattgcgctt
    37681 tttactactc ttgaagtagt tccataagct gcaaatacaa attcagcatc ttctgttttg
    37741 tacatttcgt attgtacttc attcttttcc attgcatcaa atttttcttg taaatgccaa
    37801 caatggtctt ctagtacttc tggttctaag tatagagagt ttattacatt aggttttctt
    37861 tttcctttag ttccaacagt tgcccaatct ttaggaggca attctctttt cttctcagga
    37921 gctctaaatt caactggctc catcatttga cctatcatac catctgcaac taccataact
    37981 ggtgatctgt aataatctgc aacgtcaaaa gcttccatta tcatatcaac agcttcttga
    38041 actgtagctg gtgcgaatac tggagttctg tagtctccat ttcctccacc tcttgtagac
    38101 ataaagtagt cagcttgtga aggttgtata cttccaagtc ctggaccacc tctcattaca
    38161 ttaagtacaa cacaaggaac ttctgctcct actgcatatg ttataccttc ttgctttaaa
    38221 gcaactcctg gtgaagatga agaagtcata actcttgctc ctgcacctgc tccaccataa
    38281 accatgttta tcgcagaaac ttctgattca gcttgaacga acgctccacc tattttaggt
    38341 aactctcttg ataaatattc tggtaattca ctttgtggag ttattgggta accgaagaaa
    38401 tatttgcaac cagcttctat agctgctttt ccaaatgctt cgttaccttt cataagtatc
    38461 ttagccatta aaattccccc ttaaatatta attaaattaa tctctttcta ctgttataac
    38521 tacatctgga cacatagttg cacaacttgc acatcctatg catttttcca tttcaaaaac
    38581 agttgctggg ttataccctt ttttgtttat tacatttgag tctaattgta tgatttttac
    38641 tggacatgct tcaacacaca atccacaacc tttacaacgt tcttgattaa aagatacttt
    38701 tcctttagcc atttacctaa gcctccttaa actttctata tatttgagct caaactacat
    38761 ccagtcttct ctcatatata attttatagg aaaaatttct ccttctaaat cttttggtaa
    38821 ctgttcaaca agattttcta aacatgcaac atatcttatc ggtatattac atttttcaga
    38881 aacttctttt gcaactttct gacctcttaa aacatcatca atagtagtaa acctaataag
    38941 atgagtatta ttaattagac ctgttacctt taatttagaa gatttttcta tttcttttat
    39001 atattggata acttcttcag aagtttgagt tttctcccta tttgcattta ttacaaaaag
    39061 catatcataa tcgccttctt ttaacaaatg actaaatctt cctataaccc tagcacctac
    39121 attatctcca cctaaatcta taacagaatt gtatgaatgg tctaccatag gagacattac
    39181 ctctgctgat actgctggta aatctaatgt tggagcatta attgagctat ctattggttg
    39241 tatacctaag gatttcatta actctttctt ttctctagtt ctaaagtata catttaccac
    39301 atctaaatcg gctatagcaa ctttgccaga aaccatatct cttaacttaa caacatagtt
    39361 catcgcaaat tcacttttac cacttccata gtgaccagtt atgattctta ttcttttatc
    39421 attagttatc ataaacttga gcctcttctt caccagttaa aactctaagt ccaccttgag
    39481 ctaatgcaat catttcatct tcacctggat aaacttttac atctgctata aatttaactc
    39541 tatctgcaat catttctgta aacatttttg aatatgcgat tccaccagtt aataatattg
    39601 cttttacatc tcccttaaga actgcagcac tagctcctat ttctttagag atttgatatg
    39661 ccatagcttc atatactaat ttagcttttt catcgccagc ttcaattctt tcttcaactt
    39721 ctctagcatc attagtgttt aagtatgcaa ctaatccgcc attacctttt attttctttt
    39781 taatttcatc ttgagtatat tttccactaa agcacatttt tactaatgca cctactggta
    39841 gtccaccact tctttctgga gagaaaggtc cttctccatc taatgcgttt gcaacatcta
    39901 ctattttacc atttttatga gctccaacag aaactcctcc acccatgtgt gcaactataa
    39961 gatttatatc ttcatatttc ttgtttattt ctctagcata tcttcttgct attgcctttt
    40021 gatttaaagc atgtactaca cttgctctac ttatttcagg cataccagaa attctagcaa
    40081 catcttctaa ttcatctaca acaacagggt ctactatgta tgaaggaaca tttacttctt
    40141 cacctatttg ttttgctatt attccaccta gatttgaagc gtgttctcct aaaactccca
    40201 cttttaaatc ttcaatcata gcagcactta ctgaataagt accacctttt ataggtttaa
    40261 gaagtcctcc tctacctact acagcatcta attcagatgt ttttactcca ccttctttta
    40321 gagcttcttc tattacttgt ttacgaaatt caaattggtc agacaccttc tcatattttc
    40381 ctatttcttc tgaagaatgt cttaaagttt tttcaaatac taaatcctca ttatcaaata
    40441 cagctatttt agttgatgtc gaaccaggat ttattgttaa gattttaaat attttgctca
    40501 tttgcctatt tcccccttaa aattatgcct ttgctgccat taaaacacct aaagctattg
    40561 agtttagttt agtttcttca ctgtctgctc tagaagttaa tattattggt gctttagccc
    40621 caactataac tcctgcattt tttgattttg agaagaatac taaagcttta tataatatgt
    40681 taccaccttc aatatctggg gctaataata tatcagctct tcctgctaca ggatgattta
    40741 tacctttatg tttagctgct tctaaagata ctgcattatc aattgcaaaa ggtccaccaa
    40801 ccatacaacc tttgatttct cctctttcat acatttcttc tagttcttta gcttcaactg
    40861 tatctttcat ttttggattt actttttctt ttgcgcatat tgcagcaact tttggttcac
    40921 ttatatctaa tgaatgtgct actgtgcaag cattttctat gatttgcttt ttagtatttg
    40981 tatcaggagc taagttcata gctgcgtcag ttacgaaaaa taatctatca tatccctcta
    41041 catcaaatac tgctacgtga cttaatacat ttccagttct aagacctact tctttattta
    41101 aaactgcttt tagtattatt gatgtgtcta ctaagccttt cattaccatg tcggcttttc
    41161 cttgtgaaac taattcaaca gattttagag atgcttctgc taaatctttt atatctatca
    41221 gttcataatt ttcgatatcc atgtctatgc tttttgcaat ttctttagtc ttttctatat
    41281 ctcctactaa aatggcattt gctatttttt cttttctagc catttcaact gccattaaaa
    41341 cttctttatc ttggcaacat gctactgata tagttttagg tcctctttct tttgcaaact
    41401 taattacttc ttcaaaactt ctcatgcatt tccacctcat cttgaatttt ttgaaataga
    41461 actttttata tctcaattaa attataactc gttcagcaac gttttcctag agataattga
    41521 aatatttaat tcaaaatatt tatttaaatc tatttatcag tcttttttca tatatctagc
    41581 gctttttaga tttaacaaat atctcattgt ccttatttta taatataact aaaataaaca
    41641 agatgcaaca aaaacataaa attaacaaac ttaattttat aggttttttt gtcgcatctt
    41701 gtaacaacta aatattcgaa atatttgttc attccacttc aacaatatta ggttctatat
    41761 ttattttttt aaattgatat gttgtttcat attttatttt atatttacta tcttcctcag
    41821 ggccttgtga taaatcaata tataatgaaa tatctttttt atttaactta ctcaaatcct
    41881 ccgttgactc tacaacaact tttatatcta catcagaaat tacatttgga ttatctatat
    41941 ttgttgggat atttcttaat tcaatatctc ccgcattata taaaaattct gcactcaatt
    42001 gttttgtatc atttaattta attgttatag agctatcctc aagagttata ccctctggaa
    42061 tttctaagct aacttctttt tcttcaccat tagccaaatc agctaagtca ataggttttg
    42121 tcttaatata acttattttg tctatatctt ctttctttcc ttttattgta acttgttcct
    42181 tacttaattt atagtttttc agtacatcat tatctttatt atcatttaga cttactctta
    42241 ttgggacaat tttttccttc aatagttcta tctttgcagt cacaaaggat gaacttaatg
    42301 ttactccttc aaccttagtt ccatcactat ttaatggtat taattgcaat tttttagaaa
    42361 aatcatctgt ctcattacca acatctagat tagctcttac acttttgact tctttaacta
    42421 gagttcttgg acctgataca tcgacaaatt ccttactaag ttcaatttta tttatatttc
    42481 tttttgaatt tccacttact tctaccttta tatctctttt ttcttttatg tttttttcaa
    42541 gatttactac taaaacatca tgcttaaatt ctagagtaac accttgagga atatttgctt
    42601 ttaaatatac ttccttctgt ccttgtattg ggtcttcaat ggttccatat acatgtatat
    42661 cttctttctt aacactttgt acctttgata aactaccttt tatatataca tctgctgtta
    42721 atttttcgtc tggatatata acaagatttt tatcctttaa atcactcata ttacttatag
    42781 atacaggtat atcttcaaac cctttagttt cttcagggtc aactacagcc ataacataca
    42841 tccataatgc aattgcactt aaaaatgata ttatttttat tttggtatta ttttttagtc
    42901 tatttatcat ttaaatatac cacctttgaa aaaacttctt gtttctgtat ttgtttttaa
    42961 attactacgt aatatattca tcattctttc tctagaaata tctctatata acttacctgc
    43021 tttagcaatt gatacgccac ctgtttcctc tgatacaatc agagttatac agtcagacac
    43081 ctcacttacc cctattcctg ctctatgtct agtacccaaa tctttactta aatccttact
    43141 ttccgtcaat ggcaaaaaac atgcagctgc ttttacctta gaatctctaa ttacaacagc
    43201 tccatcgtgt aatggtgtat ttggtataaa tatatttatt aatagttgcc tagaaatttc
    43261 agcatcaata atagttcctg tatttattat atcacttatt ctagtttctc tctccattat
    43321 gataagagct cctatttttt gtctagaaag tgaatataaa gcttctacaa tttcttctat
    43381 atttctattg agagtttctt cagaagtatt tacattcttt ccaaataaat taaactttgc
    43441 tcttcctata tgttcaagtc ctgctctaaa ttctggttga aaaattataa gagcagctat
    43501 aactccataa tctaaagctt taagagatat ccaataaacg gtatgcaatt taaaggtgtt
    43561 actaagttga gttgctaata aaagaaatat tataccttta aacacttgtt ctgctcttgt
    43621 atcttttata aacataaata ttttgtaaaa aatatacgct actatagata tgtcaattaa
    43681 atcatatata ctcattctta atattatatt aaaaaatcca tttaaaaaag aatttatatc
    43741 taacacagtt ttatcctcct gtttgataac ttcttaatta tttacactaa gtattatact
    43801 atataaatct aactctatac aaactattta acttttatgt aaataattct agttttatat
    43861 ttggagattg ttaaataatc tctaaacaca ttaaaaatat caacttaata aatacatatt
    43921 tcttattaaa gttagttttt actcaaataa ctcaaaccat caaggcgcga atacaaaata
    43981 gtactcgcgc cttaatgcag ttttatgttc taaaatatct taatatatct ttgtttaata
    44041 tatattaatt attataaatt ctataatcca cttattaact ataaacaaat acaaaatcta
    44101 aatattactt atcatcaata ttattccatt agattcattt ttatattaaa gtaataactt
    44161 atttcatatc tagattagat aaatttattt taatatctta ctataaacta tcattaaaac
    44221 ttattatgta taactagata taatctccta cagtgaaaat aatacacaat acctaaagtt
    44281 atatatccat ttcacagtat aataatttac ttagtataac ttataaatat tacattaaat
    44341 tgtcaattca ctatagtctc taatttttta taatttctta tacttattgt ttagacaaaa
    44401 atatgctcat aaatctcttt ataaataatt tcataagtta tttcaatata taaataccat
    44461 aaagtataca atataatgta tataactctt atctaattag ttatatttaa ctgatgcctt
    44521 cataaacatc ttgtagacta ttatacatta ccctccgtat gtttacaata ttaatgaata
    44581 tactgtaact ctattagaat atactgtaat ttttacatag tttaaagttt ataaataaaa
    44641 agacacaggt aatatacatg tttcctgtgt ctttttaaac aatataaaag tttactataa
    44701 tttatagtaa acttttctta atgagcgcac agggattcga accccggaca cacgccttag
    44761 aagggcgttg ctctatccag ctgagctatg cacccaagtt attatggagc gggaaacgag
    44821 attcgaactc gcgacttcaa ccttggcaag gttgcgctct accactgaac tattcccgct
    44881 tatttaattt ggcgacctgg aaggggctcg aaccctcgac ctccagcgtg acaggctggc
    44941 attctaacca actgaactac caggccgcga atggtgggac caacagggct cgaacctgtg
    45001 accccctgct tgtaaggcag gtgctctccc agctgagcta tggtcccatt tttggagcgg
    45061 gtgaagggga tcgaaccctc acagctggct tggaaggcca gaactctacc attgagctac
    45121 acccgcatat ttaattgaat tggtgactcg taggggaatt gaacccctgt taccgccgtg
    45181 aaagggcggt gtcttaacct cttgaccaac gagccgtttt aatggttgcg gaggcaggac
    45241 ttgaacctac gaccttcggg ttatgagccc gacgagctgc caactgctcc accccgcgat
    45301 attgttttat aagtgccgaa gaccggaatc gaaccggtac gagaggtaag tcccgcagga
    45361 ttttaagtcc tgtgcgtctg ccagttccgc cacttcggct tttttggctc caagggtggg
    45421 gctcgaaccc acagcctacc ggttaacagc cggttgctcc accgttgagc taccttggaa
    45481 catttaacgt ggctacgtcc tactctccca ggcagtttcc caccaagtac catcagcgct
    45541 agagagctta acttctgtgt tcggaatggg aacaggtgta tcctctctgc tattgtaacc
    45601 acataatctt tatgcttaat atttctatta agtttttaat aagtttatag aacctagcaa
    45661 tactagtttc tttagagtta
//
LOCUS       DAMJVX010000014        44037 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMJVX010000014 DAMJVX010000000
VERSION     DAMJVX010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44037
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.015"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(121..894)
                     /locus_tag="PC407_000725"
     CDS             complement(121..894)
                     /locus_tag="PC407_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HDF2496190.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="PC407_000726"
     CDS             complement(894..1280)
                     /locus_tag="PC407_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2496191.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="PC407_000727"
     CDS             complement(1305..1622)
                     /locus_tag="PC407_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDF2496192.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVKKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="PC407_000728"
     CDS             complement(1634..1894)
                     /locus_tag="PC407_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDF2496193.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="PC407_000729"
     CDS             complement(1894..2190)
                     /locus_tag="PC407_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2496194.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC407_000730"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC407_000730"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDF2496195.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDIAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKIGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKNEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC407_000731"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC407_000731"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDF2496196.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC407_000732"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC407_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDF2496197.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="PC407_000733"
     CDS             complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="PC407_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDF2496198.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVKIEGTKIKVL"
     gene            complement(8083..9189)
                     /locus_tag="PC407_000734"
     CDS             complement(8083..9189)
                     /locus_tag="PC407_000734"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDF2496199.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="PC407_000735"
     CDS             complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="PC407_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDF2496200.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11409..11546
                     /gene="rpmH"
                     /locus_tag="PC407_000736"
     CDS             11409..11546
                     /gene="rpmH"
                     /locus_tag="PC407_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDF2496201.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11624..11968
                     /gene="rnpA"
                     /locus_tag="PC407_000737"
     CDS             11624..11968
                     /gene="rnpA"
                     /locus_tag="PC407_000737"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDF2496202.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            12000..12251
                     /gene="yidD"
                     /locus_tag="PC407_000738"
     CDS             12000..12251
                     /gene="yidD"
                     /locus_tag="PC407_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDF2496203.1"
                     /translation="MNKIIYILKEISRYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            12283..12990
                     /locus_tag="PC407_000739"
     CDS             12283..12990
                     /locus_tag="PC407_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2496204.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12959..13588
                     /locus_tag="PC407_000740"
     CDS             12959..13588
                     /locus_tag="PC407_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HDF2496205.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13700..15079
                     /gene="mnmE"
                     /locus_tag="PC407_000741"
     CDS             13700..15079
                     /gene="mnmE"
                     /locus_tag="PC407_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDF2496206.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGERALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNILDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKEIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15174..17069
                     /gene="mnmG"
                     /locus_tag="PC407_000742"
     CDS             15174..17069
                     /gene="mnmG"
                     /locus_tag="PC407_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDF2496207.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVDKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQVRR
                     TRGGKGE"
     gene            17062..17781
                     /gene="rsmG"
                     /locus_tag="PC407_000743"
     CDS             17062..17781
                     /gene="rsmG"
                     /locus_tag="PC407_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDF2496208.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGRPAKNPIK"
     gene            17956..18741
                     /gene="noc"
                     /locus_tag="PC407_000744"
     CDS             17956..18741
                     /gene="noc"
                     /locus_tag="PC407_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDF2496209.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18876..19649
                     /locus_tag="PC407_000745"
     CDS             18876..19649
                     /locus_tag="PC407_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDF2496210.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19651..20514
                     /locus_tag="PC407_000746"
     CDS             19651..20514
                     /locus_tag="PC407_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDF2496211.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20547..21689
                     /locus_tag="PC407_000747"
     CDS             20547..21689
                     /locus_tag="PC407_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2496212.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVSDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21823..22398)
                     /locus_tag="PC407_000748"
     CDS             complement(21823..22398)
                     /locus_tag="PC407_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HDF2496213.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDTSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22658..23950
                     /locus_tag="PC407_000749"
     CDS             22658..23950
                     /locus_tag="PC407_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2496214.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24055..24657
                     /gene="yedF"
                     /locus_tag="PC407_000750"
     CDS             24055..24657
                     /gene="yedF"
                     /locus_tag="PC407_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDF2496215.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24730..24993
                     /locus_tag="PC407_000751"
     CDS             24730..24993
                     /locus_tag="PC407_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDF2496216.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            25008..25193
                     /locus_tag="PC407_000752"
     CDS             25008..25193
                     /locus_tag="PC407_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDF2496217.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25296..26492
                     /locus_tag="PC407_000753"
     CDS             25296..26492
                     /locus_tag="PC407_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2496218.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLEKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26706..26984
                     /locus_tag="PC407_000754"
     CDS             26706..26984
                     /locus_tag="PC407_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDF2496219.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27021..27455
                     /gene="ssb"
                     /locus_tag="PC407_000755"
     CDS             27021..27455
                     /gene="ssb"
                     /locus_tag="PC407_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2496220.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27474..27701
                     /locus_tag="PC407_000756"
     CDS             27474..27701
                     /locus_tag="PC407_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDF2496221.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27844..28164
                     /locus_tag="PC407_000757"
     CDS             27844..28164
                     /locus_tag="PC407_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2496222.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28172..29128
                     /locus_tag="PC407_000758"
     CDS             28172..29128
                     /locus_tag="PC407_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDF2496223.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29139..31133
                     /locus_tag="PC407_000759"
     CDS             29139..31133
                     /locus_tag="PC407_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDF2496224.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31134..31583
                     /locus_tag="PC407_000760"
     CDS             31134..31583
                     /locus_tag="PC407_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDF2496225.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31602..32930
                     /gene="dnaB"
                     /locus_tag="PC407_000761"
     CDS             31602..32930
                     /gene="dnaB"
                     /locus_tag="PC407_000761"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDF2496226.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33135..33443
                     /locus_tag="PC407_000762"
     CDS             33135..33443
                     /locus_tag="PC407_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496227.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33525..34814
                     /locus_tag="PC407_000763"
     CDS             33525..34814
                     /locus_tag="PC407_000763"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDF2496228.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNKIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35140..35215
                     /locus_tag="PC407_000764"
     tRNA            35140..35215
                     /locus_tag="PC407_000764"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35173..35175,aa:Lys,seq:ttt)
     gene            35222..35296
                     /locus_tag="PC407_000765"
     tRNA            35222..35296
                     /locus_tag="PC407_000765"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35255..35257,aa:Glu,seq:ttc)
     gene            35301..35376
                     /locus_tag="PC407_000766"
     tRNA            35301..35376
                     /locus_tag="PC407_000766"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35334..35336,aa:Val,seq:tac)
     gene            35410..35484
                     /locus_tag="PC407_000767"
     tRNA            35410..35484
                     /locus_tag="PC407_000767"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35442..35444,aa:Thr,seq:tgt)
     gene            complement(35867..36859)
                     /locus_tag="PC407_000768"
     CDS             complement(35867..36859)
                     /locus_tag="PC407_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2496229.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKEKLLSESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36868..37926)
                     /locus_tag="PC407_000769"
     CDS             complement(36868..37926)
                     /locus_tag="PC407_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDF2496230.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38051..39514
                     /locus_tag="PC407_000770"
     CDS             38051..39514
                     /locus_tag="PC407_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HDF2496231.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39616..40413
                     /locus_tag="PC407_000771"
     CDS             39616..40413
                     /locus_tag="PC407_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2496232.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40426..42528
                     /locus_tag="PC407_000772"
     CDS             40426..42528
                     /locus_tag="PC407_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HDF2496233.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEKEI"
     gene            42596..43075
                     /gene="rlmH"
                     /locus_tag="PC407_000773"
     CDS             42596..43075
                     /gene="rlmH"
                     /locus_tag="PC407_000773"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDF2496234.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIVLAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43224..43535
                     /locus_tag="PC407_000774"
     CDS             43224..43535
                     /locus_tag="PC407_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2496235.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43583..43900
                     /locus_tag="PC407_000775"
     CDS             43583..43900
                     /locus_tag="PC407_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2496236.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SKEVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
BASE COUNT        16600 a         5540 c         7012 g        14885 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ccttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgtcgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa acacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttttta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 gtccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttgacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctatagcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt cattctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatttctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctat cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagtt cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 atatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaaattt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccatctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcgt gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tatattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggtaatcctt tctcatatgt
     6181 ctgcttatat atctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcttcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccccttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatgtata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 tacaatactt ttattttagt tccttctatt ttaactatat ctcctgattt tatctttttt
     7801 cctcttctta attctatttc atcatttact gtcactaaac cttcttgaat taaaaatttt
     7861 gcatgacctc ctgttgatgc aatctctgct aatttaagaa attgatccaa ctttatatat
     7921 tctgattcta tagttatttc agtcataata gttcctcctt gtcactattt taaacaaatt
     7981 cctattgata cactaaaagt atattatacc ataaatatgc ttataaaagg agttgtctaa
     8041 gattagaaaa attttctaca aaagacaact ccttcatgtc tgctatatat ttgatgatat
     8101 tcttactgga agcagtaagt aaatataatt aacatcatct gttggtttaa ttatacaagg
     8161 atttacattt gtagtaaatt ctataaatat ttcctcatta tctatatttt tcaaaccttc
     8221 gataaaatat ctagaattga aagctatatc taggtaatct ccatctaaat ctatttcaac
     8281 ttcttcatac acatttcctt tttctgtgtt agaagtaatt gccattactt tatctcttat
     8341 agataacttt ataagattat ttttttctga ttgagacaat aaagacgctc tctctatact
     8401 atttaaaagt tccttagtgt ttaatttaac tctgctgtta tgttctcttg gcaataattt
     8461 tttataatca ataaaatctc cttcaagcaa tcttgtaata attttcgtat cattaattat
     8521 aaaaatagca tttttttcgt taaacccaac tttaacatta tcctctccag aaagcaaact
     8581 atttacatct ataagtgtct ttccaggaat tatgactttt atattttcag ttaaactatc
     8641 aactgaacaa ctttttacag ctaatctata tccatcaatt gctactaaat ttaagtttct
     8701 atctacaatt tctaaaagtt ctcccattaa aacaggtttt gtttggtcct gtgagatagc
     8761 aaatacagtt tgtttaatca tattttttag aatttcttga ggtatgctat ataaatcttc
     8821 ttcatttaat tctggtaatt taggaaattc ttttgctgca taacctttaa ttttaaatct
     8881 tgaatttaca caatttatgt aaatattatt ttctgaatca gtttctatct caacaaagga
     8941 atctggtaat tttctaatta tatctccaaa tagccttgca tctacaacaa catcaccttt
     9001 ttctatgatt tctgcttgta cataagtttc tattcctatt tcagcatcat atccagtaag
     9061 ttttaattga ccttcttctg tagatataag tatacctttt aataattcta ttgttgtttt
     9121 tccattaata gctttttgag caattccaat tctatttgct aggatttttt gattacaaat
     9181 tattttcaat gtggataacc tcctctggct ttttatatat aaaaataagt taaaatcata
     9241 gtagtaatag tagtaggtgt tgttgataat gttaataagt atgtataaca ttataaatac
     9301 aaagatatta acatgtgtat aagatgttaa tatctttgtt attttagact tgataatttg
     9361 ttaataattt ttgttcaaaa aaagtattaa caggttattc aagagatata aacatacaat
     9421 tgtgtataaa ttatcccttc aaatctgata taattttgtc tattttagtc ttaatttctt
     9481 cactttcttc tatatcctta gatactttgt catgagcatg aattactgtt gtatggtctc
     9541 tacccccaaa ctcttcacct attttaggaa gggataaatc agtaagctcg cgcgttaaat
     9601 acatagctat ctgtcttgga taagctattg aacgtgttct ttttttagaa ttaaaatctt
     9661 ccatctttaa attgaataca gaagatactt tttcttttat tctaagaaca tttatttctt
     9721 catttttaga agttgttata atatctttta aagcttctgt agcaagatca aaagatatta
     9781 ccctattgct aagagaagaa taagcaacca ctctagttaa tgcaccttct agttccctta
     9841 tgtttgactt aatatttttt gctatatatg acataacctc atttggcaca tctatacgtt
     9901 ctagttgagc cttttttcta agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat
     9961 cagttataag tcccatttca aatctcgatc ttagcctatc ttctagtgta ggtatgtctt
    10021 ttggcggtct atcactagat ataattattt gtttgtttgc ttcatgaaga gtattgaatg
    10081 tatggaaaaa ctcttcttga gttctttctt ttcctgctat aaattgaata tcgtcgatta
    10141 gtaatacatc tacgtttcta tatttattcc taaattcttc gtttttatcg tcttttatag
    10201 agtttataag ttcattagta aatttttctg aagaaacgta aactactttt gagtcttttt
    10261 tttgactaac aatatgatga cctatagcat gcattaggtg agtcttacct aatccaactc
    10321 ctccatacaa aaaaagtggg ttataagctt tagctggaga ttctgcaact gcaacgcatg
    10381 cagcatgtgc aaatctattg ctattaccaa taacgaatgt atcaaatgtg tattttggat
    10441 ttagattggg gtaaagaact ctataattta aatctgtgct atcagaattt aaatcagcaa
    10501 cctctttttc gctcaataca aatttaatat tatatttttt taatgacagt tgactaatag
    10561 catcttcaat taaatgtaaa tatctattct ctaagatatc tttattaaaa tcactaggag
    10621 ccagtaagat taaatcatta agatgtattt ttaatggtac gatgttttta aaaaaggtat
    10681 taaaacttac tgaagttaag tcacctttta ttaattgtag ggttttgtcc cataaagaaa
    10741 ctatatccat aatataaacc tccgaacaga ataattaata ttttccacaa agttattaac
    10801 agaaaagcaa aaatatatat aaattttaca gaataactaa atttatggat aatggtgttg
    10861 ataaattaat aatagtataa attattaaaa aaatatgaaa agatataaac aatatgtaga
    10921 taaagttata aataacataa acatacaata aatggtgatt ataactagag tattgtcagt
    10981 aatcttaaaa gttatacaca aaagtatcca caagctgtga ataatttcta ataagttgat
    11041 aacatttatg cacattatca acaagtatat ttataatatc aaaatatatg tcaaacttca
    11101 atgatttgta actaatttat ccacaatatt aactaattgt tgataaaaat ataaacatgt
    11161 taaaattact ttatttgtgt atataattga aaaaaataat ttagaataaa attaaataga
    11221 tttttgctag tattttcaat aaaaaaaata cataatatat atatggtaga ttttgtgata
    11281 taagcgtccc aaaaactcgt tgacaacagt agtaaaagta tttataatta tatgtgttat
    11341 gtttactttg agggtaaagt tattattttt ataaacggaa aataaaatct gaaaggcggt
    11401 gcaaaataat gagtaaaaga acttatcaac caaaaaagag acaaagaagt aaagaacatg
    11461 gtttcagaaa aagaatgaag acgtctaacg gaagaaacgt gttaaagaga agaagagcta
    11521 aaggaagaaa tagattaact cattaattac aaaagggccg ctgcaaaggt ggcctttttg
    11581 tgtaaataat tacaattcat agctgtgttt atggaggtta actatggact ttaataggac
    11641 taaagggttg aaaaaagact ctgattttag aaaagtatat aaacacggca aatcttttgc
    11701 aaacaaatat ttagtaatat atatacttaa aaataaatca gattatagta gagtaggtat
    11761 ttctgtttca aaaaaagtag gaaaagctat tactaggaat agagtaagaa gattaataaa
    11821 agaagcctac agattaaata ttgatgaaaa aattaagcct ggatatgata tagtatttat
    11881 agcaagggta tctagtaagg atgctacttt taaagacata gacaagtcta taaaaaattt
    11941 agttaaaaga acggatattt ctatttaatt gtatgctata aatatggagg ttaatgactt
    12001 tgaataaaat aatttatata ttaaaggaaa taagtaggta tttatcgaat ttatgtattt
    12061 atttagtgag attttatcaa aagtatatat ctcccttaaa gggacctacc tgtagatttt
    12121 atccaacttg ctcacaatat tcaatagagg cgtttaaaaa atatggattt gtaaaaggta
    12181 tgtatttaac gataagaaga cttttaaaat gtcatccttt tcatccagga ggttacgacc
    12241 ctctaaaata aaagagagta actattagga ggtataaaaa aattgaatat aataggaaat
    12301 ttcttaggat tgattcttaa ggtaatattt gaatttgtta atcattatgg tatttctata
    12361 atacttttta cgatacttgt aaagattatt ttattaccat taacaattaa gcagactaag
    12421 tcaacaaagg caatgcaaga tatacaacct cgtataaaag agatacaaga aaaatataaa
    12481 aataagccag aaaaacaaaa tgaagaaatt gttaagttat atggagaagc taaaattaat
    12541 ccactatctg gttgtttacc tttattaata cagtttccta tattaatagg attatttagc
    12601 gttttgagag aaccagttgc tcatggcgtt tttgcaaaca aagctgcatt tttagcagct
    12661 gacaatgggt ttttatggat aaaaagctta acatcaccag attatgtatt agcagtattt
    12721 tcaggagcaa gtgcatatgt catgcaaaaa gtaatgacac ccaaagatca gttacaaggc
    12781 tctatgaaag taatgacata tgtaatggca ggtatgtctt tctattgggg atttatattc
    12841 ccagcaggac ttacattgta ctggacagta agtaacttat tctcaatagc tcaatattac
    12901 ttaataatga atcctttaaa agctaaactg gctgctaact ctaaggagga aaaagttaat
    12961 gagaaaaagc ctaaaattaa taaaaagtaa gagtaaagag gaagctataa gtaaagctat
    13021 agcagagttg aatttaaaag cagaggatat agaagtagaa atacttgaaa atccaagtaa
    13081 agggttttta ggttttatag gtgctaaaga tggtacttat gaaatttttg ttatcgagag
    13141 ggaagtagat gtagctaaaa attttataga ggtaatgttg aaaaatgcaa atgttgatgc
    13201 caaagtcaat gtatctcaaa aagataattt aatcatggtt gatattgaag gcaaagaagc
    13261 tgcaagttta attggtagaa gaggggaaac tttagattct attcaatttt tgactggatt
    13321 agctcttaat aaaataaata aagattctca tacaagagta cttgtagata tagaaaatta
    13381 tagatcaaaa cgagaagaat ctttaataag atatgctaat aaagttgcta gagaagtagc
    13441 gaaaacaaga aaaactaaaa aattagatta tatgaatcca tatgagagaa gaataataca
    13501 ttcagctctt caaaatgaca aatatgtaat tacatatagt gaaggaaccg atccatatag
    13561 aagattagta attgagtgta aaagataaag tcaacctcct aaacattagg gggttttatt
    13621 ttttaatatt attaaatcat taatgattgg ttaaatgtaa acctatatgt aaaaaattat
    13681 gaattaagag gtgataaatt tgtttataga tgatacaatt gcagcaatag ctacagcacc
    13741 tggagaaggt ggtataggta tacttagaat aagtggagaa agagctttga aggttgcaga
    13801 agaaatcttt aaatctatgt cgggaaagag tattgaagaa tacaataaga gaacattaat
    13861 atatggaaat atattggata atgaaaatat aattgatgaa gttttattgg catatatgaa
    13921 aggaccaaac tcctatacag gagaagatgt tatagaaatt aattgtcatg gtggatttat
    13981 ttcggtaaag aagatattag aattaatact atcaaaagat gttagattag cagaggctgg
    14041 tgagtttact aaaagagcat tcttaaatgg aagaatagat ttatctcaag cagaggctgt
    14101 aattgatgtt ataaaagcca aaactgatat agctcatgaa gttgctcaaa atcaattaga
    14161 gggttcttta tcaaagaaga taagagaatt gagagataaa gtcactgaga tattagctca
    14221 tgtagaagtt gctatagatt atcctgagga agatattgag catataacat atcaaactct
    14281 taaagaaaaa actgatgaac ttaaaaaaga gattaaaaaa ttatatgata cagctgaaag
    14341 tggaaaaata cttagagaag gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa
    14401 atcttcacta cttaattcta ttttaggaga gaatagagca atagttactg atataccagg
    14461 aactactaga gatgttatag aagagtttgt aaacataaaa ggtatacctt taaaaattgt
    14521 tgacacagca ggtataaggg atactgatga tatcgttgaa aaaataggtg ttgaaaagtc
    14581 taaagaatca tttactagtg cagatttgat agtaatggtt ttagatgcat ctagaaagtt
    14641 gtcagaggaa gatatagaga tattagaaaa acttaaggat aaacaaacaa tagtgctgtt
    14701 aaataagaat gatttgaaac aagagattga agaagaaaaa atacttaaat atgtagaaaa
    14761 taatagtata ataaaaatat cagctttaca acaagaagga atagaagaat tgcaagataa
    14821 aatagagtct atggtatata aaggtagtat caaaaataat tctagtttag tagtaacaaa
    14881 ttcaagacat aaagatgcac ttagtaaagc atataaatct gcaacagatg ctttaattgc
    14941 tttggaacaa agtatgccat ttgactttgt agaagtcgat ttaaaaaata tatgggacta
    15001 tcttggatat attaatggtg atacagtaac agaggattta ctagataata tattccataa
    15061 tttctgtatt ggtaagtaat gtacaaaagt aaatttgttt ttattataga gatttttaaa
    15121 aaatttgaag tgtttctcat gaaacattaa ttttgaaaag aaaggaatta taaatgataa
    15181 agtttgaagc aggaaaatat gatgttatag tagtcggagc aggacatgct ggatgtgaag
    15241 ctgctttggc tactgcaaga atgggatata aaactttgat aataacaatg tctttggatt
    15301 ccatagcatt aatgccttgt aatccatcaa ttggagggac tggtaaaggt caattagtta
    15361 aagaaataga tgccttaggt ggtcagatgg gattaaatat agacaagaca tatatacaaa
    15421 gtagaatgtt aaatactgcc aaaggtccag cggtacactc tttaagagca caagcagata
    15481 aatttaaata tcatgaagaa atgaaaaaga cattagaaga tgaaccaaat cttgatatag
    15541 ctatggatga agttgtagaa atacttcatg aaggaaatgt tgtaatagga gttggaacta
    15601 aactaggatg ttcatttaaa tctaaagctg taatattggc cactggagtt tatctgaact
    15661 caaagatata tatgggtgaa gttgcatttt atgaaggtcc taatgcattg gggtatgcaa
    15721 aatatcttac agatagctta gttgagcttg gtcttagaat gagaagattt aagacgggaa
    15781 ctccagcaag agttcataga gatagtatag acttctctgt aatgtcttta caagagggcg
    15841 atgaaaaagt aactccattc tcatttatga atgaaaatat agaaaaaaaa caagaacctt
    15901 gttatttaac aagaactaca gaagaaactc aaaaagttat actggataat ttaaaaaggt
    15961 ctgctatgta tagtggagta atagaaagta ctggtccaag atactgtcct tctatagaag
    16021 ataaagtagt aagatttagt gataaaacct cacatcaatt atttatagaa ccagaagggt
    16081 taaatactaa agaaatgtat atacaaggaa tttcaactag tttacctttt gaagttcaat
    16141 tagatatgta caaaactata aagggacttg aaaattgtaa gataatgaga cctgcatatg
    16201 ctattgaata tgattgtgtt gacccaactc aactaaaaat aagtttggaa ataaagggtg
    16261 tcgagaactt atttagtgca gggcaattta atggaacttc tggatatgag gaagcagcag
    16321 ctcaagggct tatggctggt ataaatgcag ttagaaagat agaaggaaaa gagccgtttg
    16381 tattagaccg ttcagaagct tatataggtg tcttattaga tgaccttgtt actaaaggaa
    16441 ctaacgagcc ttacagaatg atgacatcaa gagcagaata tcgactatat ttaagacaag
    16501 ataatgcaga tatgagatta actcaaaaag gttatgatat aggattagtt aaaaaagata
    16561 gatatgaaag gttcctaaat aaaaaggctg ctgtagataa agaatttgaa agattaaaaa
    16621 atgaaagagt tacaccaaaa gaagtaaata gtttgttaga agaaaaagga gcaacaccaa
    16681 taaaggttgg aatatctcta tatgaatttt taaagagacc agaggttact tacgaattgc
    16741 ttgaagaatt agggaagggc gctggagaag atgtttcaag agaagttaaa gaacagtgtg
    16801 taataataac taaatatgaa ggttatatag agaaacaatt gaaacaaata gaccaattta
    16861 aaaaacttga gaataaaaag ctggatgaga aaataaatta ttcatctata gaaggtctta
    16921 gattagaggc tagacagaaa ttagatgata taaaacctat ttcaataggg caagcatctc
    16981 gtatatcggg tgtttctcca gctgatatat cagttttact tatataccta gaacaagtca
    17041 gaagaactag aggtggcaaa ggtgaataat atagaacttc tgaaaaacgg gattgaaggt
    17101 tttaatatag atacgaatga cagtatgtta gaaaaattta aaatctatag agagatatta
    17161 gttgaatgga ataaaaagat gaatcttact ggaatagaag atgaaaaaga agtgtatata
    17221 aaacattttt tagactctat ttcagctgta aagaatggat atataaaaaa tgggatgtct
    17281 ataatagatg ttggtacagg tgctggtttt cctggaatac cattgaaaat atgtttagat
    17341 agcttagaac ttactttgtt agattcgtta aataaaagaa taaacttttt agaggaagtt
    17401 agtagagtac tagagttaga taatattaca tttatacatg gaagagctga agattttggt
    17461 aaaaatgaag actatagaga aaaatatgat gttgctacgg caagagctgt tgctggatta
    17521 ccgattctta tggaattttg tgtaccattt gtaaaagttg gagggtattt tatttgctta
    17581 aaaggtccga atgctaattt agagctagaa gaatcacaaa aggctattga tgttttagga
    17641 ttggagtata ttgaaaaaat agatgtagag ttaccagagg tagatttaaa tcataatata
    17701 ttagttttta aaaaggtgat ggaaacacca gttaagtacc ctagaaaagc tggtagacca
    17761 gctaaaaatc ctattaagta atgaaaaaag taatgaaaaa attattattg gtaagcttaa
    17821 ttatttctta ggaaataatt aagcttaccg taaaaaaaca aaaataaagg aattattaaa
    17881 gtagatgcag aaataaattt gtatgtacat atagagaaca aaaataattt atatctaaaa
    17941 taagagaggt atgttatgga agataaaaga gttatggaga tacctataga agatgtagtt
    18001 ccaaatccat atcaaccaag aaaaatattt tcacaagttt cattggagga attgagcaat
    18061 tctataaagg tatatggtat aattcaacct ataacagtta gagcaaaaga tggcaaatat
    18121 gaactaattg caggagaaag aagattaaga gcagctaaat tagcagagct taaaaccata
    18181 ccagcaatta taaataatat gaatgatgaa tcttctgctg ttctagcgtt attagaaaat
    18241 ttgcaacgag aagacttaaa tttcattgaa gaagcaattg gttatgaaaa cttaataaaa
    18301 gaacatgctt ttactcaaca gcaattagca gaaaaattgg gcaaaaatca atctactgta
    18361 gcaaataagc ttagaatatt aaagcttcca aacgatataa aaatgaagct aatagagaat
    18421 aatttaacag aaagacatgc tagagcattt cttaaactac caagtgaaga tttaatgcaa
    18481 agtgtgttag acaaagtcat aaaaaatgaa ttgactgtta aaaaaacaga aaaattgata
    18541 caagatgtat tagaagaaac taaagttcaa gaagagcctg ataaaaaaca gaatataaag
    18601 ggtgctatga gtataagaat ttacataaat actataaagc aggcctttga tgctatatca
    18661 aatacaggaa ttgatgctaa gtataatgaa atagataagg gagattatat ggaagttgtt
    18721 gtgaaaatcc ctaaaaaata gagattactg cttaccagta atcttttttt gtaatataag
    18781 ccttgtaata taaattgatt tatagacatt atttatactt aaaaaacacc tattcaacat
    18841 ttaaaattaa tttaattctt aaagggggga acatcatggg caaagttata gctgtattca
    18901 atcaaaaggg tggagtcggg aaaactacaa caaatgtaaa tttaagtgct agcttaggaa
    18961 ctttgggaaa gaaaatatta gtattagatt tagacccaca aggaaataca actagtggat
    19021 atggtattaa taaaaacgaa gtagaaaata caatctatga aattatgtta gatgggttac
    19081 atattaaaga agcaataata agtactgaat ttgagaatgt agatgtagtt ccatcagcta
    19141 ctgaactatc aggtgcagag atagagctta catctaaaac taatagagag tatattttaa
    19201 aaaattccat aaaagcggta atagatgaat atgattatat atttttagat tgtccaccct
    19261 cactggggat gcttacaata aattgtttaa ctgctgtaga tagtgtttta attccaatac
    19321 aatgtgaata ttatgcttta gaaggtgtta gtcaattaat ggagactata aaattagtaa
    19381 aatcaagatt aaatgctgat atagaaattc aaggtgttgt tttaagtatg tttgatggaa
    19441 gagctaattt atcaatacaa gtagttgaag aagttaagaa atactttaaa ggtagtgtat
    19501 atacaacttt aattccaaga aatgtaagac ttgcagaagc tccaagtcat ggaaaacctg
    19561 taatttatta tgataaaaga tgtagagggt ctgtagctta tttggagcta gcagaagaat
    19621 ttatagattt agaagaggag gaatggtagt atggagaata agtctaaaag aagtaataga
    19681 ttaggtagag gtcttagtgc attgatacca gaaattaaag gagaaacttc agaaaaagaa
    19741 atagtcaaca tagatataga caaaatatat ccaaacgaag ttcaacctag aaagcaattt
    19801 gatgaagaaa agataaaagt attgtcggac tctataaaaa attatggtgt tttacagcca
    19861 atagtggtta aaatggatga aaataataaa tatatgataa tagctggaga aagaagattt
    19921 agagcatcaa aacttgcaaa taaaaatcaa atacctgcta taattaaaga tatagatatg
    19981 aaagatataa tggagatagc cttaattgaa aacttacaga gggaagattt aaattcaata
    20041 gaagaggctc tagcatataa gagtttaata gagcattaca atgtaactca ggaagaaata
    20101 tcagaagctg ttgggaagag tagacctcat ataactaata cactaagact tttaaatctt
    20161 ggacaagatg ttatagaaat gatagacagt ggtagaatta cagcaggcca tggtaaggct
    20221 ttattgagaa ttgcagataa agatttgcaa cttcaaatag cgaaaaagat agaagaagaa
    20281 gaactttctg taagagaagt cgaaaatata gctaaaaaaa tatctgaaaa taaacaagaa
    20341 gaacctaaaa aatctaaacc taaggatgta tttattttag atgttgaaga taaacttaga
    20401 aatatatttg gaacaaaggt gaatatttct aaaggaaaga agaaaggtaa aatagagata
    20461 gaatactata atgatgatga tttaaatagt attgtatcaa tgcttcttga atagaacaaa
    20521 aatataaaac agagaggaag attgttatga tttatttaga taatgctgca actacatatc
    20581 caaaaccaga aagagtgtat aatgctgttt tagattgtat gaaaaattat tgtgcaaatc
    20641 caggtagagc aggccataaa cttgctatga gagctgctag ggagatatat gatacaagag
    20701 agaatatagc taagttgttc aatgtaagta atcctatgaa tatagtattt acatctaatg
    20761 caacagattc tttaaattta gcgataaaag gtgtattaca agaaggagac catgtaataa
    20821 caacgtctat ggagcataat tcagtaataa gacctataaa agcattggaa aaaaggggta
    20881 ttgaaaatac tgtagtaaaa tgcgattatg agggattttt agattatgaa gatttagaaa
    20941 aatcaataaa gagtaatact aaactgattg taacaacaca cgcatctaat gtatgtggaa
    21001 ctttaataga tataaaaaaa gttggagaaa tagctaaaaa acataatata ctatttttag
    21061 tagatgcatc acaaacagca ggtgtttatg atatagacgt aaatgaatgt aatatagata
    21121 tgttggcaat gccaggtcat aaatgcttat ttgggcctca aggaacaggt atattatatg
    21181 taagagaagg gttgaactta aatatattaa aagaaggtgg aacaggtagt aaatcagaag
    21241 aaatagttca accagagtta ttcccagata agtatgaatc aggaactcat aatactccag
    21301 gtatagcagg tcttaaccaa ggtatactat ttatatttga aagaggaatc aacaatataa
    21361 gacaacatga agaagaatta tgtcaatata tgatagacaa actagaggaa gtatcagata
    21421 taaagatata tggaccaaag gatagtaaaa aaagagcatc agtaatcgct ctaaatatag
    21481 gagatatgga ttctggagag gttactttct tattagatag tgattataat atagcaacta
    21541 gatcaggaat acattgttca ccactagcac ataccacttt aggtacttta aaacaaggtg
    21601 cagtaaggtt cagtatagga tatttcaaca caaaagatga gatagataaa gcagtggaag
    21661 ctttgaaaaa aatttcaaaa aataaatagc atgagattta ataatataga aataaaaaaa
    21721 ggggatattt caaaataaaa atttgaatca tctccttttt tgtacaaaaa agaaaagggg
    21781 ctcctaagaa cctctaatct atttgataat tgtttcaaga aattacttta agtaatctcc
    21841 ttcttcaaga actatatgac cactttcgta attagaacca tcaacagtaa tttttatttg
    21901 ttcaacattc atatttttta tgtaagtacg agcaactgaa tcaagcatta aagtttctgc
    21961 atcactacct aagtttgact ttgtaaagtc agagctaaca tctaatatac cagtttttac
    22021 accatcaatg tcttttgtag taaatgaatt taattttgca gattctggaa caactttaag
    22081 tttttgaagt tcttcaaata aagtgttctc atcaatttta tttaaatcaa ctttattttc
    22141 aattaaatct gtcttatcta catcaaaaga gtaaatagta gctgattgag ttttagtttc
    22201 ttcttttgga gtaggtttag ctttgttatt aggaacctct ttttcagtat tgttttcttc
    22261 ttttggttgt tcaacatttg tattgctctt agtttctttt ttagatgtat cacttccatt
    22321 ttgagcatta gtacaaccaa ctgcgaatat agatatagat aatatactta acactaacat
    22381 tattttctta tttttcataa aaatttctcc tctcttaaaa actattctta tatataatta
    22441 tagtcgttat gagcaaatat atgaatacaa aatagtaaca aatttaaaaa atgatttatt
    22501 taaattaata aaactcaaaa taaactaaat tttagtcatt tttatgggaa atcatttaat
    22561 gtacagtgaa aatattgtaa aataaaagta gtgtttttaa aataaatata agttataatt
    22621 atattataaa aaaatgtctt caaaaggtgg gaatagtatg gaaattttga gcttaggaga
    22681 aaagattaaa aaattaagaa aagaaaaaaa catgacatta aaagaattag ctggagatag
    22741 gataacagca gcacaaataa gccatataga gagggataaa tctcatacta gttatgaact
    22801 gttggaatat ctaggcgata agctagatgt aagtgtagaa tacttattag agaccaagga
    22861 aatgcaatca aaaaagataa ctgataattt aatactacag agcgaaattt atataaaaga
    22921 aaatgaatta aacaaggctg aggatcaaat aaaagaagtt ttagatatat gtaagagata
    22981 cgatttaatg gagaactatg gcagatgtaa ttttttaatg gctaatatta atttgaaaaa
    23041 atctaattat aatgatgcta tagaaaactt tgaaaaggca ttgtacttct ttataaaaaa
    23101 tagtgatagt gaaaatatac ttaagtgtta tttaaacata gggaaaatat atatgcagga
    23161 agacttttat aaaggtgcta taagccactt taattttgca gaggaaattt tgtcagagaa
    23221 taaatttgag gatgttaaca tacacaagga actatatagt aagatggcat attgttacat
    23281 aaaactagat aatccaaata tgtcattgaa gtacatagaa aagataaatg aaatagataa
    23341 tagaaatgat agaaaagaag atgtcgatat tttggtgtta aaagcaaata atatgcttga
    23401 gattggaaaa tatgaagaat caagagatta ctttaaaaaa gcactaaaaa tacttgagag
    23461 tgaggataat aaaactggat tagcaaatgt gtatttaaca atttgtgatg tttacagaaa
    23521 aataggtgaa acagatagag tacttgaata ttcacaaaag gtatatgaca taaagaaaaa
    23581 tgatgaagat gaatacatga tgtcaggatt atttaagata atagaagcat atatagataa
    23641 agaagattat gatttagcta gaaaatattg taagatagcc ttagcttcat caataaaaaa
    23701 taaaaataaa ttcaatgaat ataaagcatt gagattctat gctaatatgt ataaaaatca
    23761 gaatgaagta actcttgcaa ttgaatatct gattaaatgc ataaaaatag tatcagattt
    23821 aggcaacaat agaatattgg ccaatttata tatagactta ggtcaattat attcaaatat
    23881 atcaaaagaa aaagagttag aatattatca aaaaggtgta tttatgtaca aaaatttaga
    23941 aataatgtag ggtaaataaa cctataaggg tatagaaaca atctataaca agcaagataa
    24001 tatctatgat aaaatataaa ttgtatgaat atttttaagg agatgaatca gacaatgagt
    24061 ataaaaatag atgcgagagg gcttgcttgt cctaaaccag ttataaatac aaaaagagaa
    24121 ttggataacc tagaagaagg tgttgtagtt acaatagttg ataatgaaac agctaaggag
    24181 aatatattaa aacttgctaa atctttaagt tgtgaagcaa atattgttga cgaaaaagag
    24241 aattttatat ctatagaaat tagaaaaggt aaaaatatac tagataaaca aaatgttgta
    24301 gataataaag aaagtgaatt agacaataca tgtatattta tatcttcaga taagatggga
    24361 cttggaaatg acgaattagg aaaagtgtta ataaaaggat ttatatatac attaacagaa
    24421 tctaaacctt atcctaagta tgtactttta gttaatggag gagtaaaatt aagtgctgaa
    24481 aatgaagaaa ccatagaaaa tctaaaaata cttgagaata tgggtgtaga agttttatct
    24541 tgtggtactt gtttagatta ttataattta aaagacaaat tacaagtagg tacagttaca
    24601 aatatgtata ctatagttga gacattaaag aatgcatcaa ataccatttc tatataatgt
    24661 atgtttgttt aataatataa aactaataat aaagattaac tcaaatagat agtcagaaag
    24721 gattaagtaa tgaatcaaat gtatatagtg tcatttaatt cgacacatca tgcgataaga
    24781 tcagaaaagt tatttggtga aaatagtcta aaagtaatgg ctcttccaac accaagagaa
    24841 ataacagcaa gttgtggaat atcaattaaa ttttcttttg aagatatgga aaagataaaa
    24901 actattttgg ttgaaaataa tgtagatata aaaggaattt attgtataag taaattagaa
    24961 aatggttcaa aagaagtaga gaagttagat taggaggcta attatttatg ccaatggatt
    25021 taaaaatagg tgatattgtt gaattaaaaa agcaacatgc atgtgggtgt aaagagtttg
    25081 aaatcataag aacgggaatg gatataaaga taaaatgtac taaatgttca aggctaataa
    25141 tgcttgatag agagacctta gaaaagagag taaaaaagct aataaaaaag tgataaaaat
    25201 ttaacactta aaaaatgtca aataccgtat attaatatcc aaaattttgg tataataaga
    25261 attgaagaaa acattttcat gggggtgctt taattatggc agttaaatat gcaaaaagaa
    25321 tgcaaggatt acaaggatca gaaatacgtg agcttttaaa acttactcaa caaccacaaa
    25381 taatatcttt tgctggtgga atgccggctc cagagttatt tccagttgag gaaatgaaaa
    25441 aagtatcagt tgcagtgctt gaagaaaatg gaagatcagc tatgcaatat actacaacag
    25501 aaggatatga accattaaga gaaaaaatag cagctagaat gaatgataaa aacaaaacta
    25561 atgtaaacaa agatgatata ttagttacaa gtggttctca acaaggtcta gattttgcag
    25621 gaaaagtatt tattgatgag ggtgatgtaa ttttatgtga aagtccatct tacataggag
    25681 ctataaacgc atttaaatct tatcaaccta aattcataga tgttccaaca gattctgatg
    25741 gaatgataat ggaagaatta gaaaagatac tagaaacaac tgatagaata aaaatgattt
    25801 acgtaatacc tgacttccaa aatccaacag gaagaacttg gccacttgaa agacgtaaaa
    25861 aattcatgga aatagtaaat aaatttgaaa taccagtaat agaagataat ccatatggag
    25921 atttaagatt tgaaggagag actttaccat ctttaaaatc aatggataca aaaggattag
    25981 taatattctt aggaactttc tctaaaatat tctgcccagg atacagatta ggatggactt
    26041 gtgcttctcc agaaatttta tctaaattta actttgcaaa acaaggtgca gacttacaag
    26101 catcaactat atctcaaatg gaagtaagca aatttatgga tatgtatgac ctagatgcac
    26161 atgttgacaa aataaaagca gtttatgtta aacgtagaga tgtaatgctt aagactatgg
    26221 aagaagagtt cccagaagga ttagtattta ctcatccaga aggtggatta tttacatggg
    26281 tagagcttcc aagtaattta aatgctaaag aattaatgcc aaaatgttta gagaagaatg
    26341 ttgcttatgt tgctggagga ggattcttcc ctaatggtgg tagagaaaat actttcagac
    26401 ttaactattc aaatatgcca gaagaaaaaa taatagaagg tataaaaaat atagcagctg
    26461 tattaaaaga agctatgggt gtagaagctt aattaaaaat aataacttat atataataca
    26521 agggatggtt gttgccatcc cttgtttgtt tgaaaagtat ataattcacc ttatactaaa
    26581 ctttgtccaa aatttaaatt gacagaaata agaataaatg ataaaatgtg taggtatgag
    26641 attttagtct catttctctg ctctaagagt gtagagccgt taagtccaaa gggaggtgaa
    26701 ttatagtgag aaattatgaa ttagtttatg tggtaaagcc aaattctgat gaagaagtaa
    26761 gagaggctat actaaacaaa gttaaggaag ttgttgcaac tgacggagaa atagttaaag
    26821 ttgatacttg gggaactaaa aaattagctt atccaatagc taagtttaca gaaggtttct
    26881 atgtattagt taacttcaaa tcagcagtag acgttcctaa agagatagac agaaacttaa
    26941 agataaatga aaacgtaata agacacatga tagttgttgc ttaataactg gttttattat
    27001 aaaaaatagg tggtgttttt atgaatcaag ttgtattagt gggaaggttg actagagacc
    27061 ctgaattaag atacatccca ggtacaggaa caccagtggc atcctttaca atagccattg
    27121 atagagacta tgtaaaaaaa gatggatcta aagaaactga ttttatacct gttgaagtaa
    27181 tggggaaatc tgctgaattt tgtgctaact acataactaa aggtagatta gtagcacttc
    27241 aaggatctat aagagtagac aattatcaga cacaatcagg tgaaaagaga acttttacaa
    27301 aagtaagtac tagatcagta caagctttgg atagtaaaaa taaatcggaa aattcatata
    27361 gagagagtgc tccagctttt gagccaagct ttgaacctca aggtttagac ccacaaggtt
    27421 ttcaagctat agatgatgac gatataccat tttaaaaagg agggaaaaaa gtcatgatga
    27481 ataagaaaag acgtaagaaa aaaagagttt gtcaattttg tgctgacaaa aacgctaaaa
    27541 tagattacaa aagtactcaa agattacaaa agtatataac tgaaagagga aagatattac
    27601 caagaagaat atctggaact tgtgctaagc atcaaagaga attaacagtt gctataaaaa
    27661 gagcgagaaa tatagcactt ttaccatata cattagacta aaaaaatgag ccttgtgctc
    27721 attttttatt atatgtattt atatatctaa atgaggcgag ctataaagct cgtttttttg
    27781 tttatatact taaatatatt attatttatg atataatatt atatattaag gagggttgta
    27841 aacgtgaaaa tagataaagg ttctgagata acgagaaaca taaagataat agaatggatg
    27901 aaaacagaaa tattaatgag tgtaagtgat ttgtttaatt tattgtttaa aggggtaaaa
    27961 ccattagatg aagcaattca agatacttta gcaaatataa taatgataac atatctctta
    28021 gcaaagagat taggaattag ttttagagat gtggattata aagtcaagga aaaaataaaa
    28081 attggtatag atgaggacca tagcgttgaa agatggtatg gagatttatc taatttaaaa
    28141 aaacatatgg ataataggga gtgatattaa attgaataat aaaattagac tatctaaggc
    28201 aatgtctata atagttttag ctataataat cgctttagta atagcctata ttccaatgct
    28261 aggaatattt agtatgttag cagctgttcc atatgtaatt ataggtgcta ttactgatag
    28321 aaagtattct cttatatcta ttttggttac attttgtgta ctgattctat ttgcagacat
    28381 ttcttatgca ttaaatatat gtataatgta ttctttacca ggtatagtaa taggaaagat
    28441 gctaaagaga agctttgaac aagaggacag taataaattc gaaccgatat atggtggaac
    28501 cataatattt gtactttcaa tgcttgtata tttttttgta ttaaagacat ttttaaatgt
    28561 gaatctatta gatgaagttt ctaagatgat atcagaaatt gtaaatattc aaaaaaatag
    28621 tttttctgca actgaactta aagtgtttga taaaatgaag ccagatgaga ttgtaagtta
    28681 ttttacaaat atgctaccaa tgatgttatt tttacaaggg ttgttatcag cgtttgtcac
    28741 atattatttg agtgtatttt ttataaaaag gattacaaag atgaatataa gatttcctaa
    28801 atttgctgat ttttatttac caggaaatgc tatactcaca actttcttac tttatttatt
    28861 agtcttattt attcaaatta ttggttcaaa attatataca gaattaataa tgactaattt
    28921 acagctagtt tttaatttaa tgtttgtaat tcaaggtata gcagtttgca tttatttctt
    28981 aaagaaatgg ataagacagg gaccaaataa aattatgttt ttttcaggga taattttatg
    29041 tttatttgga tttatgggaa tatcttttgt tggtatggta gatagtataa ttgactttag
    29101 aaaggtaaga agctataaat ccacttagga ggatagaaat gagtaacaaa caaaccttta
    29161 aattaaatat gccagaaata aatttatata taattgttat tggtatttct agtataattt
    29221 tgctttatta caacttgtat gtagggtgtt tatttttctg tatttttgta tatatggttt
    29281 ttcacaattg gagaactact aatattagac gtcatgaatg gacagagtat attcaaaatt
    29341 tatctttaga tatagatgaa acaactaaaa aagccataat aaatcttcca ataccattat
    29401 gtattttaga atttgatgga aatatatcct ggtataatgg aaagttttat gatatgattg
    29461 gtcaaaaaga tttacttgat aaaaatatag aggatatagt taaaaatctt aatttaagaa
    29521 aagtattaaa tgaaaataaa gagatgtaca cagaaataaa ctataaagaa aaagaatata
    29581 caataattta taatgttata aaaaatgatc aagaaaaaaa tcctaaatat ttgatgatat
    29641 tatactggat agacaagact gaatatttaa aagtaaaaca aaattatgat gatgaaaaaa
    29701 atgcaatgat gctaatacaa gtagatggat atgatgaagt attaaaaagt gcagcagaag
    29761 ataagagagc tttaattaat gttgaagtag agaaaatact ttctgcttta gaattaaatt
    29821 caaatggtgc attgagaaga acttcaaaag acaaattctt cttggtaatg cataaaaaag
    29881 agctaaaaaa attggaagct gagaagtttt ctattttaga tacaataaga catattgatt
    29941 atggaaataa tcttcctgtt actataagta taggaattgg tatagatgga gatacattaa
    30001 atgagaacct aaaacttgct acgggtgctc tcgatttagc acttggtaga ggtggtgatc
    30061 aggctgttgt aaaaacaaaa gataaatttg tgttttatgg aggaaaatct aaagctgtag
    30121 aaaagaaaac caaagtaaaa tccagattaa taggtcatgc tttaagagag gttatacaac
    30181 aaagtgacca agtttatata atggggcata aatatcctga tatggatgct atgggagcag
    30241 cagttggtgt ttatgatata tgtaaatcct gtaacaaaac tgcaaacata gttttacaat
    30301 ctgtaaatga atctatagaa atatttataa ataaaataaa tgaaaataat tactataaaa
    30361 agctgtttat tggaaaagag gaagctattg acaactgtac taaaaataca ttagttgtag
    30421 tagttgatac tcatagacca aattatactg aatgtgaaga actgttaaag ctatcagaga
    30481 aggtagtagt tatagaccat catagaagag gtgtggagtt tataaatgat gcagtgcttt
    30541 tattccatga gatatatgta tcctcaacgt gtgaaatggt tacagagtta gttcagtata
    30601 tggatgaaga tgtaacaata aataaactta cagctgaagg gcttttagca ggtataagtc
    30661 tagatactaa aaactttgca ttcaaaacag gtgttaggac ttttgaagct gcttcttacc
    30721 ttagaaaagt tggagcagat actatagagg taaagaaatt cttcaattct gatgtaaaag
    30781 atttcataat aaaagctgaa ataattcaaa gtactaaaat aattaataat agaatatgtt
    30841 tagcatattc aagtactgag atagatagta taaatgttat aatagctcaa actgctgatg
    30901 agttattaaa tatcaaagaa gtagaagctt cctttgtatt aggtgaaaaa gatgatacta
    30961 tatttataag tgcgaggtct ttagggcaaa taaatgtaca tgtacttatg gaaaaattag
    31021 gaggcggggg acacatagat atagctggag cacagttaaa aaatgtttct ctaaaagaag
    31081 cttataagat ggtaaataaa ataatagagg agtatttgga ggaggaagaa tagatgaaag
    31141 ttatattatt aaaagacgta aaaggaactg gtaaaaaggg agaaatgaaa gaagtaagtg
    31201 atggatatgc aagaaatttc ttatttccta aaaagatggc tgtgcaagca gatagtgtag
    31261 caataaaaga attaaatgaa aagaataaat ctaaagaaat aaaagcacaa aaagagtatg
    31321 aagaagcagt tttattaggt aagcaaatgg aagaaattaa tatagaaata tattcaaaag
    31381 caggagaagg tggaagatta tttggttcaa taacttctaa agaaattgct gagcaattaa
    31441 agaaacaaaa agacatagac gtagataaaa gaaaaatatt attagatgaa ccaataagat
    31501 ctttaggttc aactttcgta gaaataaaaa tacatcaaaa agtaactact aaaataagag
    31561 tggatgtaaa agaaaaacag taataactag aggtgattaa gatggaagat atgacgagaa
    31621 ttcctcctca tagtgtagaa tcagaacaat caatattggg ttctatactt ctagataaag
    31681 atgctataat aacggttaca gaaactataa agcctgatga tttctataaa gaggctcaca
    31741 aaataatata tgaatgtatg ataactttaa gtaataaagg tgagcctata gacttgataa
    31801 cattaacaga agaactaaga aaacaagggc atcttaatga tattggagga atcagttata
    31861 ttacaagtct ttctactata gtaccgacga cttcaaatgt aaaatactat gctgatatag
    31921 taaaagaaaa gtctgtactt agaaagctta taaaagcttc taatgaaata ataaatctag
    31981 gatatagtgg agcaactaag attgaagatg ttttagaaca agctgaaaag agtatatttg
    32041 atatatcaca ggagaaaact agtgatgatt ttaaatctat aaacttagtt ttgatggatg
    32101 cctatgatat gatagaaaaa ttatatacta acaagagtga tgtcactggt ataactactg
    32161 gttttaaaga tttaaacaaa aaaataaatg gtcttcaaag gactgattta atattaattg
    32221 cagctagacc agcaatgggt aaaactgcct tttctttaaa cttggttcaa aatgcagcct
    32281 taaaaggtga tgcctctgta gctgtattta gcttagagat gtctaaggaa caattagtac
    32341 aacgtatgtt atcttcacaa tctagtgtag aattaaaaaa aataaagact ggaacactta
    32401 atgataatga ttggcctaga attatagatg ctatggcagt attatcagat gccaaaatac
    32461 acatagatga tactcctggt ataaaaatat cagaactaag gtcaaagtgt agaaagttaa
    32521 aaatagaaaa aggactagat ttagtgctta ttgattatct tcaacttatg gaaggtgaag
    32581 gtaataatga aagcagacaa caagagatat ctaagatatc aaggtcacta aaaatattag
    32641 caaaagaatt aaactgtcct gtagttgcac tatctcagtt atcacgtgca ccagaacaaa
    32701 gagcagacca tagacctatg ttatctgatt taagagagtc tggagcaata gaacaggatg
    32761 cagatatagt tatgttctta tacagggacg aatattatca tgctgattct gagagtaaaa
    32821 atataggaga agtaataata gcaaaaaata gacacggtga aactggttct gtggaacttg
    32881 tttggcttgg tgaagttcaa agatttgggg ataagttgag agacctataa tctaacgaat
    32941 atccacagaa cgtcctttct ttctacagga aaaatgtgga tacatgtcac taaatatgtc
    33001 caaacattga aatttagcta aaaaactatc cacactatcc acagatagcc tgttgataag
    33061 tgtgaataag tagataaagc aagcttttgg cttgctttat ctgtgtataa atattaatta
    33121 ttaggaggaa taatatggaa aaaataagct taaaatatat atatcccaat ataattaaag
    33181 tacttgatga aataaactta tttagagtaa ttgacaataa tttaagagag tctattgttg
    33241 tttatgctaa taatgtggat aatcagtatc atataaacat gactaataca aactttggaa
    33301 acataattaa catatgtaaa ttagaaaaac ttttagatgt ggataaattt atggaaaaag
    33361 ttataaaata tgaaaaagaa ataatagaga aagaagagtt ttcaaaaata gaagaatata
    33421 tgttaaatat tggagaatac taaaaaagtt gaaaaaaata cgaatgtttg atagaataag
    33481 taagtaaagt gttcgagagt atataaaata tagaggtgaa ttacatgaaa acagttgcaa
    33541 ttgttggttc tcaatgggga gatgaaggta aaggtaaagt tatagattat cttgctactc
    33601 aagcagatgt agtagtaaga gggcaaggtg gaaataatgc aggacataca ttagttgtag
    33661 aaggtaaaaa atacgcatta catctaattc catctggagt tttaaatcca aatacagtga
    33721 atataatcgg aaatggaata gtgtttgacc ctaaaggatt tttagaagaa ttggaaatgt
    33781 ttaaaacgga taatataagt acagaaaata taaaaataag tgatagagca catgtcatat
    33841 tcccatatca taaagaacta gatgcattgt cagaagaagc cagaggcgat ttaaagatag
    33901 gtacaacaaa aaaaggtatt ggaccttgtt atatggataa aacagagaga tctggtatta
    33961 gaatatgtga cttaatggat aaagataaat ttgctataaa attaaaagct caaatagatg
    34021 ctaaaaataa aatagttaaa aatatatatg gaaaagaaga attatttgat tttgaaacta
    34081 tatataacga atatctagga tatgcagaac aaataagaaa atatgtggca gatacgtctg
    34141 ttattgtata tgatgcagta agagcaggta aaaaagtatt gtttgaagga gcacaaggga
    34201 ctttattaga tttagattta ggaacttatc catttgttac atcttctcat ccaacatctg
    34261 gtggatttgc aattggtgca ggtataggac caaatatgat aaaagacgtt gttggtatag
    34321 taaaagcata tactacaaga gttggagaag gaccgtttgt tacagagcaa ataaatgaaa
    34381 ctggagataa gataagagaa caaggacatg aatttggggt aactactggt agaccaagaa
    34441 gatgtgggtg gtttgatgca gttatagtta agtatgcagc tagagttaat ggattgacta
    34501 gtatttcatt tatgttactt gatgtattaa ctggatttga taagataaaa gtttgtacgt
    34561 cttataaaat gggcgataag ataataacgg atttcccagc ttcattagat gatttagcaa
    34621 aatgtgaacc tgtatatgaa gaattagatg gatggaatga agatataact caaatagata
    34681 actttgatga ccttccagaa aatgcaaaga aatatgtagc aaaaatagaa gaactagttg
    34741 gagtaagtgt agatatggtt tctgttggtc caaatagagc tcaaactata ataagaagaa
    34801 atatatttgc ttaatatttt atcaattaaa taatttaaaa tagttgaaga agagtatctc
    34861 aagataatgt ttatagttat tagagatgct cttttatatt tgacttacat tgatttttta
    34921 ttaagttaaa gctacttata catattctag ctgaaattaa agttgaattt atgtagatac
    34981 atataggttt tttaaaaaga attttgttga aaataaatgg ctaaagatga ttttaattat
    35041 attctaaaaa aagtaaaaaa aactattgac taaaaattgt atagacgcta taatataact
    35101 tgtgagtgaa agaaacgacg aaaaaagtcg aaaaaatgtg gtctattagc tcagtcggta
    35161 gagcacctga cttttaatca gggtgtcccg cgttcgagtc gcggatagat caccaaaata
    35221 tggctcgttg gtcaagaggt taagacaccg ccctttcacg gcggtaacag gggttcaatt
    35281 cccctacgag tcaccagtat gggactatag ctcagctggg agagcacctg ccttacaagc
    35341 agggggtcac aggttcgagc cctgttagtc ccaccaaatt ttgaattaga gataaatact
    35401 agattatatg ctggtgtggc tcaacggtag agcagctgac ttgtaatcag caggttgtag
    35461 gttcgattcc tatcaccagc tccacaaagt acataaattc aaattaatat aattttctat
    35521 gttacaggat acttttaagt atcttttttt aatttcattt ttattgtatt aaaaatgaaa
    35581 ttaaacatac aacaaaattt tgaattactt gcaatgtgat agttttaaat ataaaattta
    35641 gaatcattct agcatagtat tatgtaatat agcaaattta atttatagta tcaataaata
    35701 tagactacta agaatgtaaa tgatttgtta gattaagata cattagatat gtaaatagaa
    35761 aaataaaaaa tatgttttaa tttatgtggc ataagtttta aacagagaag caagtttgct
    35821 cttacctctc tatttaaaaa taaaaaaatg ttttttaagg gctattttat ttaaatcttt
    35881 cccatctcaa atcatctcca gtaaattcta aaatcctaaa cttatctaag attctagaga
    35941 gagttctttg agtgtaagta tttcctattt gagatggtgt taagttggtt gaaataatga
    36001 tttttttacc agcaacaagt cttgtattta caatattgaa gatttcccca ctagtaaaag
    36061 aattattaag ttcagttcca aggtcatcta ttattaataa atcacaatca aacaagtttt
    36121 tataattatc ttcacttatt tggttatttg tgtctcttct aaatttataa tcttcaagaa
    36181 tatctaaaat tctaaaagaa gtttgataga ttacaacatt tcctttatct aacaattcct
    36241 ttgcaataca attgcacata taggtctttc ctagtccagt agaaccataa aacagtagat
    36301 tttcactatt atccttctta aaatcatgaa caaaattttc gcagatactt aaattattta
    36361 gcatattttc tctaggagat atttccccat cagagccttt cttaggagaa aatatattca
    36421 agttaaaatt cgaaaaattt tcctgactta aaatcctatc taaattagac attttataag
    36481 cttcatttac aatttcttgt ttgaggcaag aacatttttc tccattaggt aaaaatcctt
    36541 tatctttaca agaaatacat tgaaacttta tttccaagta atcaagaggt atgttacttt
    36601 cagataaaag tttttctttc tttactttta aagactctat attctcttta gtcttcttta
    36661 caatttcatc tttagatttg ggatttaaaa gtactatctt agctaaactg agacctattt
    36721 tagaaatttc atcatctata gattttatct cagggattct attatatacc tcattttttc
    36781 tatgttctaa taagagttca ttatcatctc ttctcttagc atatttagca agtatttttc
    36841 ttattttatc ctcattcatt taatcaccta cttaaacttt tctttttgac tttttttaat
    36901 aatctcatcg agctcatcag aggtatactg agtgaatgtt tcgttgaaat tatgaaactt
    36961 agtttttttg taagtattgt tgttttgagt agtattaatc tgcttcttat taatattctc
    37021 cttaactata cgttcttcct ctttttgctt taaatcattt aaatttttta tatttttttc
    37081 attccaattt ttaattatac catttatata agatatactt gggtttgaaa tattcttaga
    37141 cttagaacat gcatttatta tcaaatctat atccatatta aatttatcaa accaggtgtc
    37201 cataagttct ttttctgatt ttgatggttg tcttgaaaat cccaattcat tgaaaatcgt
    37261 tttatataaa atatatcgtt cacttcttac caaaaagctt tcttcaacat cttttggcgt
    37321 atataaattt gaatcatacc aatttctaat aatgccttca atgtatttaa ctggctttga
    37381 tgtaccagtt ttatctttga cgtattcata tgcgcaaagt atcatatcgg ggctcatatt
    37441 gtatttattc attatatcca taatactcat cttttcactt gggtcgagat atcgcccaac
    37501 tattttattt atagaattaa acatttttct aatacttgga ttttcagaag tagatacaac
    37561 cttatctgta ttagatttta tagatgaatt attgcttaat atattctcaa tatagaaatt
    37621 ttttaaatct aaaaattcta ttgaataatt aaaattatca tattctccat tgtcatgtat
    37681 ttttataatc ttcttttctt cccagaattt ccaagcagat aacacatcag acaatggtat
    37741 atttaggttt ttggctattg aattattgtc gaacttagga ttagaagtaa tatcacatgc
    37801 ttgtctatat cctaaaagat aaacttttac gtataacccg tccgccattg gcataaatat
    37861 atctataaag atattgggaa tagtgatttc acctaaatct atttcattgc tttctttaaa
    37921 aaacatttta tcacctcaga ttaaattata tcatttttta tattatattt gtctgtaaac
    37981 aaataaaagg tcacaagatt agcatgtaaa tattttctaa aagtattcat tttgaaaagg
    38041 aggagatatt ttggttataa aattcaacca aaaagtaaga agaacattat cagtagcgat
    38101 tctatttatg gtattaataa taggttgctt tatagaaaag gatgacagtt tagaaacata
    38161 caaagaaaaa actaattcaa aaataaatca gtataatatg gatgttatat ttgatgatga
    38221 gactaagagg ttaatgtgca atcagaatgt tgactacata aataatacaa aatcaaacat
    38281 agataaaata tatttccata tatatccgaa tgctttttcc aagaaagatt ttgctccttt
    38341 tgaaaaaagt gaaatgacaa gggcttatcc taatggattt aacgaaggat atatagatat
    38401 aaagaatata ttaaataata atagtaaaat ggagtataaa attaaaggag acaaaaatga
    38461 catattagaa atagaattag ggaaagagtt aaaaccaaat gaaagaatgt cattagatat
    38521 aaaatataac gtaaaaatac caaattcaat tggaagattt ggatatggag aaaatactat
    38581 aaatgttact aattggtttc ctatagcttg tgtaaatgat gaaaggggat ggaatttaaa
    38641 gagttatgaa actattggcg acccatttta tagtgagact agcgattttc atgtgaaact
    38701 attgattcca aataaatata aaattgctca tacaggaaaa cttattgatg ataaacatga
    38761 taataagaaa atgctatatg aaatagaagc tgaaaacgtt agagattttg catttatact
    38821 aagtagtaaa tttgatgtaa acaaagtaga ttcaaaggga gtagctatca atacatataa
    38881 tttaaataaa aagctttcta aaaaagcaac tgaagtggca aaagattcaa taaatatatt
    38941 tagtgaatta tttggaaagt atccttacaa agaattttct gttgtagcaa gtgacttttt
    39001 tatagggggg atggaatacc ctatgcttgt tatgatagac caaagtttat ataatgaaaa
    39061 aaatgaattt ttactagaat atgttattgc ccatgagaca gcacatcaat ggtggtattc
    39121 tgctgttgga aatgatgaaa taagtgaacc ctggttagat gaagcactta ctgaatattc
    39181 tacaatagtt tattttgaac aaaagtatgg taaagatatg gcaaacaagc ttataaaaac
    39241 tatggagata cagacaaaaa gttatttgag tgaaaatata tttaagccag caaatgagta
    39301 taaaaattct actgaataca gcttgaatgt ttatacaaaa ggcgcaatag catttaatga
    39361 aataagaaaa gaagttggag ataaagtatt ctttgagact ttaaatgaat actataataa
    39421 atataagcat aaaaatgcta atggaagagc ttttgtggaa ctatggaata gtaaaggtgt
    39481 agatataaat aagattataa gtgaatgtaa gtaaatagtg aatgtaagta aattttgtgc
    39541 agagtattaa attttattta atactctgtt tttttggtat aatataattt ataaggcaga
    39601 aaaagaggtg aaggaatgct aaagtattgt tcagtaggga gtggtagtag tgggaattgc
    39661 cactatatag ggtataaaaa tactaatata ctagtagatg caggattaag tggcaagaga
    39721 ataactacag gtcttaaaga cattggtgta gatgcagata aattaaaagg aatatttata
    39781 acacatgagc atgttgacca tataaaagga gcaggtatat tatccagaaa gttcgatata
    39841 ccagtatttg ctaatataaa aacatggtgt tctatgaagg acaaattagg tgacatcaaa
    39901 gataaaaata tgaaagtatt tgaaaatgat aaaacctact cattaggtga tatcatagtg
    39961 agaccttttt caatagaaca tgatgcgtct gatccagttg gatataattt ttatacagag
    40021 agtgatgaaa aaatgtctat agctactgat ataggatgta taactcaaaa tataaaaaaa
    40081 catctatata aatctaaatt agtagtattg gaatcaaatt atgaccctaa tatgcttatg
    40141 atgggttcat atacatatgc tttaaagaaa agagtaatgt caagtactgg tcatttatcc
    40201 aatgaagatg cagctaattt ctgcgttgaa cttattaatg aaggtacaga atctatatta
    40261 ttagcacatt taagtaaaga aaataatttt cctgagttag catatgagac ttcaaaagga
    40321 gtattagcat caaatgatat agtagttgga caagatgtaa aactagatgt actatcacga
    40381 aatgatgttt ctaatgttta tgagatgaag tagaggtgaa taactttgct aaagagaaat
    40441 tttgaaaatg ttgaggataa taaaatttta tactctataa agcagggtat gattttagca
    40501 atacctgcta ttatgactgg ttcaacggct cttgtaattt tgaatcttcc aataaaggct
    40561 tatcaagaat atttatcagg cttgtttaat ggagaggtta caaatattct taactttata
    40621 aatgattcta ctttaggaat tatttcatta ataatattat taacaataag ctatagttat
    40681 ggaaaaatat atgggagcaa gtatacagta cttgttccta tagttgcaat gtgctctttt
    40741 ttagtatttt cacatggtaa tgagcttaat tcttatatag aaatatttaa gacaaagtgg
    40801 ttatttacat ctataattgt ttctatgaca tcttctgtat tgtttgtaaa acttacagaa
    40861 tcttttgtta caagtgtaaa gtttcataca gaaggggctg atgcagactt taatatggta
    40921 gtttcggcta tagttccttt cataatagta gtatttttgt tttctatttt tagagtgata
    40981 atgataagtc taattggcag ttcaaacttt caagatatat tttataattt attttcaaat
    41041 gtttttaata aaatgggaac aaaccttatg agtgctctgt tgtttatttt tttaatgcac
    41101 tttatgtggt tttttgggat tcatggtagt aatgtattag ataccgtcgc aaaaaattta
    41161 tttgaaaata gtatggcaat taacataaat ttagttaata ataatcaatt accaacagaa
    41221 atttttacta agaccttttt tgatacaatg gttttattgg gaggatgtgg ctctttacta
    41281 tgtttagtaa ttgcaatttt tcttagtgaa aaacgtataa atgtaagaaa gctagctaaa
    41341 attgcttcaa taccagctct ttttaatatc aatgagatgc tagttttcgg gataccaatt
    41401 gtttttaact atattatgtt tgtacctttt gtaattactc ctataatctt aaccattaca
    41461 agttatgtgg ctatgtctac aggtattgta ccatgtacag tttcagctgt agaatggacc
    41521 tcacctatat ttttaagtgg atatatgtct acaggttcaa taagaggtag tttgttgcaa
    41581 gtatttaatt tatgtgttgg tgttatgatt tatattccgt ttataaaaat gtcgcaaaat
    41641 agatatacat atatgtttaa gaataatata gaaaatttaa caaacttatt aaaaaggtat
    41701 gaattaacag gtgaacagcc taatcttcta aaccataatg gtaaaatagg ttcaatttca
    41761 aaaatgttgg catcagactt gaagtttgct atgaaaagag gagaaataga acttttttat
    41821 caaccacaag tcaattataa tggaaatgtt gtgggagcag agggattact tagatggaaa
    41881 cattcaatag gagggttcat ttatccacca ctagttataa ttttggctaa agaagaaggg
    41941 ttcttagatg agcttggaga gtatattatt aataaagcat gtattgattt aaagaagagt
    42001 gaaaagctat tgagaaatcc tgttaagttc tctgtaaata tatctccaga ccaacttgat
    42061 gaccctaagt taccggagca aataaaaaat atcattagta gtaatgatat aaatcctaat
    42121 atgttaggta tagaaataac agagcaaatt gcattatcag gttcacaaat aattattgaa
    42181 cgtatcaatg cgatacataa cttgggaata aagttaatta tggatgattt tgggatgggg
    42241 catagctctc ttatatatct tcaaaataat aactttgata tagttaaatt ggatggttca
    42301 ttagttaaag agatacttac aaataaaaga agtagtgaaa ttataatgac tattgtaaat
    42361 ctttcaaaaa atttagattt tgatatagtt gttgagtatg ttgaaaatat agaccaaagg
    42421 gataagttat atggtttagg atgtgaaata tatcaaggat attattatag caaagcaata
    42481 ccttttgaag agtttgtaga atatgcaaat caagaaaaag agatataatt ttataagagc
    42541 ttgtaaattc tagtaatgca tagatttgta ataaaattaa gtttaggaga aatatatgaa
    42601 tataagtata gtagtagtag gtaaaattaa agaaaaatat ttaaaattag gtattgatga
    42661 gttcaaaaaa aggctttcta agtattgtaa gttagaaatt atagagttgg atgatgaaaa
    42721 agctccagag aatttgagtg ttaaggaaat ggaaattata aaagacaaag aaggaaagaa
    42781 aattttaggg aagataaagc ataatagtta tgttatagtc ttggctattg atggtaaggg
    42841 tttatcctct gaagaacttg ctaagactat gagtgattta gcagttagag gtaatagtag
    42901 tctctgtttt ataataggag gttctttagg gctttctgat gaggttttag gaagggctga
    42961 ttataggtta tccttttcta agatgacttt tcctcatcag atgatgagat tgatattgtt
    43021 agaacagatt tatagggctt ataggataaa taatggagaa ccgtatcata aataaatgat
    43081 acggtttttg ctaatttgac acttaataat attaaaataa caaaaaagta ttgaaagtat
    43141 attgcattta aggtagaatt aaataaaata ttataacatt ataatgttta tatagttgtt
    43201 aataaaaatt tggaggaaaa tatatgataa aaataatgtt ggcctgttca gcaggaatgt
    43261 ctacaagttt attagttact aaaatggaaa gtgcagctaa agaaaatggt atagagtcgc
    43321 aaatttgggc aataccagaa tcaactatac aaaatgaaat agaaaaatgt gatgtattat
    43381 tactaggacc acaagttaga tatgtattac ctaaggctca agaaattgct aagccatata
    43441 atataccagt agaggttata aatatgatgc actatggaac agttaatgga gaagctgttt
    43501 taaatagagc gatagaactt aacaacacta agtaatataa attataatgt atttttaata
    43561 tataaattgg gagaatcaaa ggatggatga aaagattata gaaatatctt ttaatataat
    43621 aggttatgca ggtgaaggta aagggttagc ttttgaagca ataaaagagg ctaagaatgg
    43681 aaatatagaa aaagcaaaag aattattaaa agagtctaag gaagttataa ataaagcaca
    43741 tagatatcaa acagaattaa tacaaaacga agctagtgga aacaggactg aaataagtgt
    43801 catacttata catgcacagg accacctaat gaatggtatg aattttcaac agttagctga
    43861 agaaatcgtt gatttacatt tgaaattaca agataaataa aaattaaaga gaataaatta
    43921 agagagttct taaaagatac attatgtgta gcacactaat tgttagatta ttgattcaat
    43981 atttagtgag taatacatta ggattatttt gaggactctt tttatattta gtttaaa
//
LOCUS       DAMJVX010000015        42446 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMJVX010000015 DAMJVX010000000
VERSION     DAMJVX010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.016"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            143..493
                     /locus_tag="PC407_000776"
     CDS             143..493
                     /locus_tag="PC407_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2496237.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            512..2155
                     /locus_tag="PC407_000777"
     CDS             512..2155
                     /locus_tag="PC407_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDF2496238.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2236..4293
                     /gene="recG"
                     /locus_tag="PC407_000778"
     CDS             2236..4293
                     /gene="recG"
                     /locus_tag="PC407_000778"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDF2496239.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIRNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMERFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4352..4906
                     /gene="rsmD"
                     /locus_tag="PC407_000779"
     CDS             4352..4906
                     /gene="rsmD"
                     /locus_tag="PC407_000779"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDF2496240.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNVKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4908..5405
                     /gene="coaD"
                     /locus_tag="PC407_000780"
     CDS             4908..5405
                     /gene="coaD"
                     /locus_tag="PC407_000780"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDF2496241.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5409..5933
                     /locus_tag="PC407_000781"
     CDS             5409..5933
                     /locus_tag="PC407_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496242.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6277..8196
                     /locus_tag="PC407_000782"
     CDS             6277..8196
                     /locus_tag="PC407_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2496243.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQERFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISKEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8209..8496
                     /locus_tag="PC407_000783"
     CDS             8209..8496
                     /locus_tag="PC407_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2496244.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            8629..9888
                     /locus_tag="PC407_000784"
     CDS             8629..9888
                     /locus_tag="PC407_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDF2496245.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISKFIGSLFGGIL"
     gene            9891..10631
                     /locus_tag="PC407_000785"
     CDS             9891..10631
                     /locus_tag="PC407_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2496246.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQILVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            10954..12264
                     /locus_tag="PC407_000786"
     CDS             10954..12264
                     /locus_tag="PC407_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2496247.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            12362..13279
                     /locus_tag="PC407_000787"
     CDS             12362..13279
                     /locus_tag="PC407_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2496248.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            13295..14104
                     /locus_tag="PC407_000788"
     CDS             13295..14104
                     /locus_tag="PC407_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2496249.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            14161..15909
                     /locus_tag="PC407_000789"
     CDS             14161..15909
                     /locus_tag="PC407_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HDF2496250.1"
                     /translation="MEISYVLKDCTFKHNKYSVIEAETWKEKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSTISWKGLENRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLNIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDKSKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMSKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDENDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIENLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            16172..17194
                     /locus_tag="PC407_000790"
     CDS             16172..17194
                     /locus_tag="PC407_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HDF2496251.1"
                     /translation="MFGGINIKRIGIIIMFFLSIAFIGFYLAIRGNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEEFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNVPKDSNIVV
                     SFHYYEPNDFAFQGNIYHNGFEHLSNITWEGTNEQMDYLKKRFDIVENWANKNKVKIF
                     LGEFGVTKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            17199..19505
                     /locus_tag="PC407_000791"
     CDS             17199..19505
                     /locus_tag="PC407_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2496252.1"
                     /translation="MKKNSKKILITLTIITNIVYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPVISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKNKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKIRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKNIYPKEIALEINENIVLECDLCEGKIDERGLLYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTIPVSNPSEKKEKSIDEFEPMEYI"
     gene            19477..20592
                     /locus_tag="PC407_000792"
     CDS             19477..20592
                     /locus_tag="PC407_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496253.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSAKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSRITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQKINLSIQSKNMLEFRLKEGSEIFLEDKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEKSNVVLDSSMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     SFLHNIKITGNEEVQIPVLGQSISQYEVLGLKRDSKIKYSLMGKQDNVSISQNGKLTL
                     SNKAEPGQIILQVNVDDKFKIGRKIALTESWSINKKDKDGVPYTLVSPEQSPTVQYMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            20610..22028
                     /locus_tag="PC407_000793"
     CDS             20610..22028
                     /locus_tag="PC407_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HDF2496254.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCVYNIGVLFIFKYLAFAL
                     RNISELINTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTMQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMLGG
                     NGVIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            22043..23251
                     /locus_tag="PC407_000794"
     CDS             22043..23251
                     /locus_tag="PC407_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496255.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNICYSGKDMIQEIKNSVAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSEEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKENYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCK"
     gene            complement(23332..24720)
                     /locus_tag="PC407_000795"
     CDS             complement(23332..24720)
                     /locus_tag="PC407_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF2496256.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFTIHLIFITLNGLNPITYIRNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSLYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFGKKNIS"
     gene            25062..26396
                     /locus_tag="PC407_000796"
     CDS             25062..26396
                     /locus_tag="PC407_000796"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDF2496257.1"
                     /translation="MRVVIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFDDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(26525..27442)
                     /locus_tag="PC407_000797"
     CDS             complement(26525..27442)
                     /locus_tag="PC407_000797"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDF2496258.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLNSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            27650..28534
                     /locus_tag="PC407_000798"
     CDS             27650..28534
                     /locus_tag="PC407_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HDF2496259.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRIKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGKKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            28747..30588
                     /locus_tag="PC407_000799"
     CDS             28747..30588
                     /locus_tag="PC407_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDF2496260.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKNKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDYVVSKKVDEIKEQQEKILDKVIGATTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGERITSIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPILKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNQGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            30892..31599
                     /locus_tag="PC407_000800"
     CDS             30892..31599
                     /locus_tag="PC407_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2496261.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            31593..32903
                     /locus_tag="PC407_000801"
     CDS             31593..32903
                     /locus_tag="PC407_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2496262.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMAQE
                     NYLKDTTESEETGYLVEYNEYFKEGTFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEKLEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            33020..33703
                     /locus_tag="PC407_000802"
     CDS             33020..33703
                     /locus_tag="PC407_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2496263.1"
                     /translation="MKILYTENLSKHYGKGENLVRALDNVNLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            33696..36083
                     /locus_tag="PC407_000803"
     CDS             33696..36083
                     /locus_tag="PC407_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2496264.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFELIKGKLPTKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKNDK
                     KIEKEFVISGILKTSEISEAGKYYYAIISELYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGGFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKNNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNLIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKAVISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            36362..37504
                     /locus_tag="PC407_000804"
     CDS             36362..37504
                     /locus_tag="PC407_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2496265.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIVGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLDRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALKLTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNENLLNLFNKYYF"
     gene            37523..38047
                     /locus_tag="PC407_000805"
     CDS             37523..38047
                     /locus_tag="PC407_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2496266.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYITEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            38147..38965
                     /locus_tag="PC407_000806"
     CDS             38147..38965
                     /locus_tag="PC407_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2496267.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSELFAKKLEESLNPTQSVEKIVLYLEEMLLSILEEDLISNKSV
                     KLLNSFIYDNRYSNIKAFCDNTNINIKTLERICLKYTGYTPKILKRIYRFKMASSQLI
                     YNYKDNELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYNYL"
     gene            39043..39354
                     /locus_tag="PC407_000807"
     CDS             39043..39354
                     /locus_tag="PC407_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDF2496268.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(39657..41660)
                     /locus_tag="PC407_000808"
     CDS             complement(39657..41660)
                     /locus_tag="PC407_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HDF2496269.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     NFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(41813..42445)
                     /gene="recX"
                     /locus_tag="PC407_000809"
     CDS             complement(41813..42445)
                     /gene="recX"
                     /locus_tag="PC407_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDF2496270.1"
                     /translation="MITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNEIN
                     EEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKSYN
                     LVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENAMY
                     LAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT        16608 a         4550 c         7356 g        13932 t
ORIGIN      
        1 tttatccaaa aacttttcta ggtttaaatt ataccatatt tatagtatga tatgattata
       61 attttatata ataaataagt aaaaatagta aaaaataaat taagaaatgt ttttaataaa
      121 aataagctta agggggtcaa ccatgagtgc aaaggtaacg aatcaatatg gaaatataga
      181 aatagataat caagtaatag ctcaagtcgt atatagagct gcaatggaaa gttatggctt
      241 agtaggttta gcatataagt ctaaaggaat agtagagcta ttaaaaggcg agaacgcaac
      301 taaaggtgta agagttgagg aagtagaaga agatgccata gcaatcgaac tttatgttat
      361 aatacaatat ggtacaaata tatctactgt tgccaataat ataatagata gagtaaagta
      421 tgtagttgaa aaaatgactg gcgtaagagt tactagaatt gatattaatg tacaaggaat
      481 aagagttaag taatttcata ggaggaaaca aatgattcaa tatatagatg gtaaaaggtt
      541 aagggagatg tttatttcag gtgcaaacaa tctccaaaac aataaagagt tagttgacaa
      601 gttaaatgta ttccctgtac cagatggaga tacaggaact aatatgtcat taacaatatc
      661 ttatgcctta aaagaattag caaaagttga aaatgataat atatcagata tcggaaaagc
      721 attatctaaa ggttctttaa tgggagctag aggaaactca ggagttatac tttcacaaat
      781 aatcagaggt atagctaaat ctatagaagg aaaaagtaaa ttatcaacag aagatttagc
      841 aaaagctttt aaaaatggtt cagacacagc atataaagct gtaataaaac caattgaagg
      901 aactatactt actgtagtta gagaaagtgg agagtttgca ataaaaactg cgaaaaaaga
      961 gaaagatgta gtaaagtttt taagtatgct tgtgaaggaa tcaaattctt cattagaaag
     1021 aactccagac cttctaaaga atctaaaaga agctggtgta gttgactctg gtggaaaggg
     1081 tcttgtactt atatatgaag gaatgttagc ttctataaaa ggcgataaca tagaaataaa
     1141 aaatgttgac ctagatacta atatttcaac aagtatggat tttgcaaaga gtactactag
     1201 cacagatgat ataaaatatt gctattgtac agaatttatt ttagaaagtt ctaaggtaga
     1261 agatactaaa ataagagaca taatgatggc ttatggtgat agtctagcag tagttggaga
     1321 tgatggagtc ataaaggtac atgtacacac taatgaccca ggaaatgtac ttcaagaagc
     1381 tctaaagtac ggtcaattat taactataaa aatagaaaat atgaagttac aacatgaaaa
     1441 tacattactg gatgtagaag aaaagaaaga aaatgatagt gaacctttag aagaagagaa
     1501 agagtttggg tttatagcta cttctatggg tgaaggtttg gcaaatatat ttaaagattt
     1561 cggagttgac catataatag aaggtggtca aactatgaat ccaagtactg aagattttat
     1621 gaatgctata aagcatataa atgctaaaaa tatatttatt ttcccaaata acagcaatat
     1681 aataatggct gctaatcagg caaaagaatt gagtgataaa aatataatag ttatacctac
     1741 aaaaaatacg cctcaaggat ttgctgcatt agtaactttt aatggagaat taagcgaaga
     1801 tgaaaataaa gaagctatga tgaatgcttt aaactcagtt aaatcaggtc aagtaacttt
     1861 tgctgttagg gatacagtga tgaatgaaat tgatgttaaa gaaggtaata taattggaat
     1921 tgcagaagga aatttattat cagcaggaga ttatgtggat gaagtaacat caaaccttat
     1981 tgaaaagttg gttgatgaag atactgctat aataacatta ttctttggtg aagatgttac
     2041 agaatctcaa gcaaatgaac tacgtacttc tttagaagaa aagtttgaag atgtagatgt
     2101 agagttatat tatggaggac aacctcttta ttattactta atttcagttg aataatgatt
     2161 ataagaacct gtatgaccaa gtagctttta taaaattggt tgtacaggat ttttgtgtag
     2221 taggtgatat atttgttgga tttatataaa gatgttcaat atgttaaggg aataggacct
     2281 aaaaaagcag ataaattaaa taaactgggt atatttacac taaaagacct attgtattat
     2341 tttccaagac aatttgaaga tagaaataat ctaaagaaaa tagcacaatt agaagatggt
     2401 gagaaagtta cgataaaagc agtaatatca agtataaata catttagccc aaaagaaggt
     2461 atgactttaa caaagattga tgtaaaagat gagacaggct cggctaaatt agtgtttttt
     2521 aataaaagtt atataaaaaa tacttttcgt ccaggagatt caatattagt ttttgggaag
     2581 gttaagaaaa aatttaataa cttagaactt acatcatgtg aattagaata tcttactaat
     2641 tcacctaaga atacatgtag atttatgcct gtgtaccagc ttacatacgg tgttacaaat
     2701 aaagaaatta tgagtataat tagaacagta cttgaagata aagaattaat tatacaagaa
     2761 tatatgccac aaagaattat agaaaaatat aggctatgta gtatagattt tgcagttaga
     2821 aacatacatt caccaagtag taaggaatca ttaaaaatag ctttatatag aattgtattt
     2881 gaagaattac ttatactaca actagggttg tttgtattta aaagtggcag aaataaagaa
     2941 gatggaataa agtttgaaac aagtaaagac ttaaaaaaaa taataagtgc tttaccattt
     3001 aaactgacta aagctcaaaa cagggcatta gatgaaatta tacaagatat gaatttagag
     3061 aaaataatga atagattggt acaaggtgat gtagggagtg gtaaaacagt agtagctctc
     3121 ttagcacttg caaattgtgt tttaaatggt tatcagggcg ctttgatggc acctacagag
     3181 atattagcag ggcaacatta catttcactt actgagtctc taaaagattt tggaataaat
     3241 gtaggtctat taataggtag tcttacaaaa aaacaaaagg atacagtatt ggaacaaata
     3301 aggaataatg agattgatat attaataggt acacatgcac taattgaaga taaggttgaa
     3361 tttaataata ttggtttagt tataacagat gaacagcata gatttggtgt tatgcaaagg
     3421 agcaaacttt cattgaaagg agcaaatcct gatatattag taatgacagc aacaccaata
     3481 ccaagaactt tagcacttat actctatgga gatttagata tttctattat agatgaactt
     3541 cctccaggaa gacagccaat agaaacaatt gctattgaaa aaagtaagcg agatagagca
     3601 tataataacc ttgtaagaag agaggttgaa tcaggaagac aagtgtacat tgtatgtcct
     3661 ttggtagaag aaagtgaagc tatagaagct aaatcagcag ttgaacttgt agaggaatta
     3721 agagctgaat atttccacga tttaaggctt ggattgcttc atggtaaaat gaaatcgagt
     3781 gaaaaagacg aagtaatgga acgttttaaa aataaagaaa tagatatttt agtttcaaca
     3841 acagttatag aagttggtgt caatgtccca aatgcaacat taatgataat agaaaatgca
     3901 gaaagatttg gacttgcaca attacatcag cttagagggc gagttggaag aggtagtcat
     3961 aaatcgtatt gtgtactaat ttatgattct aaaacagatg tatgtagaca aagaatggct
     4021 ataatggaag agaccaatga tggttttaag atttcagaga aggatttaga aataagagga
     4081 cctggagaat tttttgggac aaggcaacat ggtcttccag aacttaaagt agcaaactta
     4141 tttaaacata taaaaatatt gaagttagca caacaagaag ctcgatatat attgggtgaa
     4201 gataataatc tacaattgaa agaaaacatg gcactcaaaa aagaaattat agataaattt
     4261 aaagatacat tgaaagaaat ttcattaaat taattttaaa ttatgttaaa atattactaa
     4321 tgtaataatg tgctgaaggg agattgtcat attgagagta atttcaggaa aagcgagagg
     4381 attaaaacta aatactccaa aaaatgaaga tgtaaggcca actacagata gagtaaaaga
     4441 gtctctattt aatatgataa attcatatat aatggaaagt gaagttctgg atttatttgc
     4501 tgggactggt tctttaggaa tagagtgctt atctaggggt gccaagtcat gtacttttgt
     4561 agatatcagt aaggaaagca tagatattgt taagtccaat gtaaaaaagg caagagttga
     4621 aagtgaaagt attattttaa accttgattt taagactgca atagataaac ttaaattaca
     4681 aaatagtaaa tttgacataa tttttatgga ccctccgtat tataaaaata tgtttataga
     4741 agctattgaa aaaattgata attctaatct attgaacgaa gatggaataa tagttgtaga
     4801 acatgatacg aatgatttat ttccagataa gatatgtaaa ttggagaaaa ctagagataa
     4861 gaaatatgga aatactacat taacttttta taagatggag gcataatatg gaaaataaac
     4921 ctagaaaagc tatatttgca ggaagttttg acccaattac taatggacat ttagatatta
     4981 tttgcagggc ttcgaagtta tttgacgaat tacaaatagg agtattaaat aatcctaata
     5041 aaaagggtct atttagcttt gatgaaagag taaaacttat agaaaaaagt acaagccatt
     5101 taaataatat aaaagttgtg acttttgatg gtttattaat aaattactgt caggaaaatg
     5161 ggattggagc attagttaga ggtgtgagaa gtggtgctga tgttgattat gaacttcaga
     5221 tggcacatat gaatagagag cttaatccag atatagagac tataatttta ccaagttgta
     5281 caaaatattc atttataagt tcatctttaa taaaagaggt tttgctcttt gatgctgata
     5341 taaaaaattt ggttccaaag attgttttgg aagaattaaa gaaaaaaact agtgggggta
     5401 attgatatat gaagatagat ttagaaatga ttgaactatt tgagcagtta gaggagatat
     5461 ttagaagtgc ttctacaatc cctttctcac ataaatgcac agtggataaa gatgaggttt
     5521 tagcaataat taatgatata agaactttaa taccagaaga agtaacacag gcagtatgga
     5581 ttaataaaga aagaaataaa ataataagtc aagctaagca agatgctaca aatatagttg
     5641 aacaagcgaa aaaagaagct gagagaatac aacaagaata tcaagataat atagaggaat
     5701 taaagaaaaa ttctgaagat gtagtaaagg catatgtaga atccagcgaa cctgttgttc
     5761 aagcagacca aagagcaaaa gatatagttg atagagctga gagaatagcc aatgagataa
     5821 gaattgggtc aattgaatat gcagaggatg tgctttctag tgttgagtat aatttaagag
     5881 aaatattaga agaagtaaaa agagataaag ctgaacttag acctaaaaaa taaattttaa
     5941 ataaataaaa atggagctat cacacaaaaa attccaattg atgtgtgata gctctctttt
     6001 aatatagcaa ttttttgtta tataattgtg ctttttagat tacttatatc ttattttgat
     6061 attataatgt agcttaatct tttatttgtt gaaatcaact tatttatttg tataaataat
     6121 ataggttatt ttcaaatttt tttaataatt ttaccactgt cttcttttag attagggtta
     6181 gactgtgcaa aattataaaa taatactaaa tcatgtgcaa ttatttggta tggcactaca
     6241 tgtagaaaat atagataata aaattaagga ggtagtatga aggaaagggc taaaaaaata
     6301 ttagatttta ttattaaaaa taacaatgta acatcacaag aactacaaga aaggtttaat
     6361 gtttcaaaga gaactattta ttacgatatt ctagctataa atgaacaact aggaaaaagt
     6421 ggtaatataa aaaatgtaaa acacaaattt atatttgaag gtaatttatg tgatgcaagg
     6481 aaaataattt caactgaaga agataaattt ttagatagtg attacagaaa aacttttata
     6541 ttaaacaaga tattacttgg cgaaaaaata tcaatagaaa aactaacaaa tgaaatgtta
     6601 ctttcaaaaa acacagtagt acaaacaata actgatgtaa aaaaatacct tcaaacaatg
     6661 gggttaagat tagagtataa gggaaagtac aaaattatag gtgatgaata cgttattaga
     6721 gagctgtttc tgataattgt tcaagaaaat gtactggaaa ttaattctat aagtaaggaa
     6781 gtaagtagtt tcgatacaaa aggacatata aaacttactg attattcttt attaaatcta
     6841 actaagtttg tggaatttct aaataaaagg ataagagatg ggaaaacatt atactcatat
     6901 aaatatctaa atgaagctaa aaaaattagt tactttagta attgtaaaga acttttatgt
     6961 gaagaagcca atgaaaatga acaagcctat atatgcactt acataagttc acttcctagc
     7021 ttaaatagtg aagttaagga agatgttgtt gaagaatatg tagataaact tatagataag
     7081 tttgaagtaa atacagcaat aaaattagaa agtaagcatg agtttaagaa aaatatttta
     7141 aggcacctcc actcttctta caacagaata cgatttaagt ttcctattag aaatccaatg
     7201 ttagatgaga ctaagtataa acatgagtca ctttataaaa ttataaaatc aattatagaa
     7261 aatgaagaag agtttcctgt atttgagggg ataagagaag aagagatagg atttatagca
     7321 gcgtattttg gagggtattt aagagggtct agggataatg gcctaagaag aaataaagtc
     7381 ttactagttt gtcctaatgg tcttatggta tcaaagagtt tggaaataca actatataaa
     7441 tacataccaa ccatagaaat agtaggtata gtgtccatta agcaactaaa agaagtaaat
     7501 gtatactacg attacataat tacaactata gatatacaaa atgtaaacaa tgtaattgtt
     7561 gtaaatcctc ttttaacatc atcagatgtt caattactta tgaacaaact aataagtgta
     7621 aaagaaaacg agaagtattt caatcttgaa ttaataatac aagctataag aaaaaatgga
     7681 gttatcaata atgaagaagc actaaaagct gatttattaa acataataca caagatagat
     7741 gaaggagaga tgtatcaacc aatgttaaag gaattaatta atgcagaaag ggtcaatata
     7801 ataaaaagtg ttagagattg gaaagaagca ataaaaatag cctcaaagcc attgttagaa
     7861 gataactcta tagaagagtt atatatagaa aatatgataa agtcagttga aaaatatgga
     7921 ccatatattg ttttagctga tagatttgct ctaccacatg caagttctaa agaaggagta
     7981 aacaagcttg ctatgtcttt actaatagta gaagatgaag ttgatttatt aggaaaacca
     8041 gtaaatatat ttatggtatt agctgctgta gataacacta ctcacattag agcattagca
     8101 tcactatcag agatgatgta cgaggaagaa aatgtaaaac taataattaa tggtgataaa
     8161 agttcaatac tagaacttat aaataagcaa aattaggagg aatgaaaaat gaagatatta
     8221 gcagtatgtg gttttggtgt aggttcatca atggtattaa aaatgacaat acaaaaggct
     8281 ctaaagcagt taggtataga atgtgaagta gaaaatacag atataaattc agctagaggt
     8341 gctgattgtg actgtatatt cacaagtcaa gaattgttaa atgaattaaa aggaacttgt
     8401 aatgtagatg tatatccagt taaaaagtac atggatttag cagaagtcac agaagttgtt
     8461 caaacttttt taagtgataa aaatgataag aaatagtata agttttaaaa agtaaattta
     8521 agttaatcag aataaaataa aaacttacta aattcaaaaa tagtaagaaa gagaggaggg
     8581 atttagtggt agaaaactta aacagttgac ctatcactaa aaaatataat gcttgatttt
     8641 atttcaaata atattttacg taatccatct atgttaattg gattaatagc agcatttggt
     8701 cttgtactac agaaaaagaa agcatctgat gttataaagg ggtcactact tgcagctttc
     8761 ggaattataa ttatggagac tggaacaggt atgttagtta gttcaatagc accaattaat
     8821 ggagctttcc aaagtataag tggtggtgca gtaacaaaag gtttaagtga tgttactttt
     8881 actgcaagtt atggtggaat tataggttta gcaatgtttg cgggtttagc tattcaccta
     8941 atgatagcaa gatttactcc tattaagaca atcttcttaa caggtcacat gctttattgg
     9001 ttcccattca tatttgtagc ttcaggagtt gaagctggat taaatggtgg agtattaata
     9061 gcttttgcag cagtattatc agcactctat tggtcaataa tgccttggtt attaagaaaa
     9121 tatgtgtttg atgttatagg agatgattct tttacattag gtcatccatc tggttgttta
     9181 gcacttatat caggatttgt tgctaaatta gtaggaaata aagaaaaatc tacagaagat
     9241 ataaacatac ctcaaagttt atcatttttt agagaaatat ctataagtgg tggaatggca
     9301 gtgtttttaa tgtttatagt tgttgggata gtaataccag gtgcgtttaa agatagtaat
     9361 gagtttattg gaacgtatgc aataaagcaa ggtctttctt ttggagcagg tcttataata
     9421 atgcttcatg gggtaagaat gctaataaat caaataatgc cagcatttca aggtatatct
     9481 gaaaaagttg tgccaaatgc actacctgct tttgactgtc caatattatt taattataaa
     9541 ccaaatgctg tattaatagg attcttagtt gctatgctaa catctacagc tatgatttta
     9601 atatgtaact actttaatgt atttaatttg ctacttatac cacttgttat aacttcattc
     9661 tttgagtgtg gagcagcagc agtaatagga gaatcacaag gtggtatacg tggagctata
     9721 ataggtacag ctatagctgc aatatttatg gtagttttag taggaatatc tgctgtaatt
     9781 tactcaggaa ctattcaaaa ttggatacta atatttggag gtaacgacct ttcattattt
     9841 ggtataatta gtaagtttat aggttcacta tttggaggaa tattatagat atgaaattta
     9901 aatacataga tgagataaga agattaattg atataataga aaacgaagaa aaagtcaata
     9961 tggaaaaagc agtacaaatc cttgttgaag caatagaaag taaaaatgct atattttcat
    10021 ttggagcttc acatgcagga atattaacag aagaattatt ctatagagca ggtggctgtg
    10081 ttcttataaa tccagtattt gcaaaatctc taatgttaga tacagcgcct ataactcaca
    10141 caagtaagat ggaaagatta gaaggatatg gtacagctct agctgataaa actccaatta
    10201 aaaaaggaga tgtagttata gttcattcag tatctggtag aaatccagta agtatagagt
    10261 ttgctatgaa agctaaggaa aataaggcaa tagtaatatg tataactaat ttaagttact
    10321 caaaaagtgt atcttcaaga cattcttcag gaaaaaaact ttatgaagta tgcgatttag
    10381 tgatagataa tcatggtcaa aagggagatg cctgtgtaga gatagatgga ttaaaccaaa
    10441 aagttagtcc aacttctact gtaatagcaa ctactgtgat gaactctatt atagctcaag
    10501 ctactcagga attagttaat aaagggctaa agaatcctcc gattttctat agtgccaata
    10561 ttgatggtgg agatgagtta aataagaaga tttttgatga atataaaagt gtaattcatt
    10621 atgaatatta aggttaaaaa aatatgttaa tgaactttga agtttatcaa caaagaatat
    10681 taataatata taataaatgc tatcatttaa aagctgtagg taaaaatcta cagcttttta
    10741 ttattgtaga ttaaaaagat gcgtaattaa attttaaatt ggagaaaata aattggaagg
    10801 tatcaattat aaatattaga aacgttgtca tagaaaaaaa ctatattaaa aaaaagtaca
    10861 tagaaaaaac tgattagaca ataaatacat gctttgataa tattttaaat ggagtgaaaa
    10921 tgtgtagaat tataatgaaa aaagaaaata aaaatgaaaa aagaaaataa aatggaggga
    10981 ataaaagtgg gtatgttaaa aaaattatgt tcactagcta tggtagcaat tatgactaca
    11041 acacttataa cagggtgttc aagtggaaat ggaaaagatt caaaaaagga tggcgaaaaa
    11101 gaaaaactag ttctttggat gccaccagta gaaaataata tgaaggaagt ttgggaccct
    11161 attttagata aatttgagga aaaaaataac tgtgaagtag atttacagat aattccttgg
    11221 gataattatt ctgaaaaatt tgcaacagct ataagtgctg gcgaaggtcc agatgtagga
    11281 tatatgtatg cagaaatgta tccacagttt atagaaagtg gagcagtaga agatttgact
    11341 aactatgcaa ctaaagagga taaagagcaa tctatttata taaaaacatc tgaaatgatg
    11401 ggtggaatgt atggtatgcc attccaagca gcaaatccag gggtattata ctataacaag
    11461 gatattttag agaaattagg agagaaacct cctaagacat gggaagattt taaaagaatt
    11521 tgtaaaaaag caacacaaga tactgatggt gatggaaaga tagaccaatg gggattagct
    11581 cagggatggg gagctaaaac ttttggtaac ttaaactgga actggtatcc ttatttatgg
    11641 caagctggtg gagatatttt taatgatgat ttaaaatcgg ttaaatttaa tgataagagt
    11701 ggtttagaag cagctaattt cttaaaagaa ttacaagcat atgtgcctga agattcttta
    11761 tctaaagata gtaatgaaat gatagaaagc gtatttggac cagggaaagc agcatttaca
    11821 ataatgttat cttctgctgc aacaagtgta tttgatgaat cattcccgga tttaaattgg
    11881 gggtttgtaa ctggactaga agataagaaa gcagcaactt ttggagcagt agaccattta
    11941 tctttaatgt catctgctaa aaataaagaa cttgcataca agttaataca acatatgtta
    12001 agtgttgaat caatgacaga atttcataaa gcaataccaa gagcaccaat aacaaaaggt
    12061 gaaccatatc aaggtgatga aagattcaaa gaaatggtgg aaaatgacaa aaatgtatat
    12121 agaccattag tagtaggacc tcatggagtt gaaatatatg aatatttatg gaaagaactt
    12181 caaactatga tttcaggaga taaaactcca aaacaagcat tagatgatgc tgcaaaatat
    12241 tcaaatgatt tattagcaca ataaaagttt aaagctgtaa tatgttgtaa aggactttac
    12301 agattaaaat tagcattgta tttataaata caatgctaat atttatattg gagaggataa
    12361 aatggaaaac ttagttagag agtttgaaga aaattcaaaa agaaaaaaaa caaaattaaa
    12421 tattaaacca tatatgtata tattgccatt gggaataatt ttagtggcgt tttatgtgat
    12481 accaataata atgtcaatat actttagttt tacaaagtat aatattatta gtccagccac
    12541 ttttataggt ttagaaaatt ataaaaaatt atttacagat gaaacattaa aagtatctat
    12601 tataaatact attaaattta ctgttgttgt agtaccttgc caaactatat tatcattgat
    12661 tttggcagtt tggattacag gaaaaggaaa tagtaaaata gcttcatttg ctaaaggtgc
    12721 tatatttata ccagtattat catctatggt tttgataggt atggtatgga gggctttatt
    12781 gaatggagaa ggttctatta tatatcaggt attaggtgcc tttggaatag aatcatctaa
    12841 acttttagga gatagtaaga cagcattacc tacacttatg tttatatcta tgtggaaaaa
    12901 tatagggtat ttcatggtaa tttatatttc agctataatg aatttaccaa aacactgcta
    12961 tgaagtagcg aaggttgatg gggctacaaa acttcaagaa tttataaaaa taactgtacc
    13021 tttattgaaa ccaactacaa taatggttgt atttttaggg tcgatatggt cattacaagt
    13081 atttgactta atttatactg tgacaggtgg aggtccaggt atatctacta tgagtatagt
    13141 tatgcatgca tttaacttaa actttaaaaa ctttaattct ggatatgcaa tgactgtagc
    13201 aaacgtatta tttttgttaa tagcagtagt ttcgatttta caaaataagc ttgtaaaacg
    13261 agacaattca gatttttaga aaggtaggta taatatgaaa acagcaagaa gattattaag
    13321 tgatggttta cttttattta tagcgtgtgc aagtttggta ccttttatat acatgttgat
    13381 aatatcattg aagataacat ataactcata tagtttggat atatcttttt caacagtaac
    13441 tcttcaaaac tatatagata tctttactaa aaaaggattt gcacaatatt tttttaatac
    13501 agcaatagta tctttttcag gtgtattatt aaatttagta tttagtacat tagcaggata
    13561 ttcttttgcg aagatggatt ttaaaggtag tgataagtta tttttattta tgataatgac
    13621 tttaattata ccttcccaag ttacaatgat accactatat ataataatga aacacttagg
    13681 atggataaat agttatcttg cacttattat gccaatacca actgcatttg gcgtatttat
    13741 aatgagacaa gctattttag gagtaccaaa agagttatta gaatcagcaa agatagatgg
    13801 ttgctcggat tttagaattt taatacaaat agtattgcct ttaatcaaac cagcattaat
    13861 tacactagca atatttactt ttatgggagc atggaacgag tttatgtggc ctcttatagc
    13921 tacaactaaa gatgcaatga gaacattaac agttggatta tctactctta agacattcca
    13981 gataactaat tatggacaga tgatggcagg ggctacaata acatttttac ctccatttat
    14041 tttctatttg attttacaaa gtaaatttgt tgaaggtgtg tctttgtctg gtataaaagg
    14101 ttaaatagtc taatttaata acataaaaga aacttatata aatatggagg aatttaaact
    14161 atggaaattt catatgtact gaaagattgc acattcaaac acaataaata ctcagttata
    14221 gaagctgaaa cttggaaaga gaaggatttg gatattgtag tagctaaagg tgaaaaattt
    14281 gcatttcaaa ttatgctaaa agcaacagaa gaatttaatt gtacaattga taaaagcagt
    14341 actatatcat ggaaaggact ggaaaataga gtaagattag cattaaatgt gcctaatagt
    14401 ttagaaaata attttagtat aaatatatta ggatatgttc aagatgatac aaaggcattt
    14461 gtgaatgatg ctattttaag ggataaagat gtgttagtag agcaatattt gcctcaaaca
    14521 ttttggattg aaggtcaagt accagaagat tttgaagaaa ataaccttaa tataggtatt
    14581 gatatattta aaagctttgg atatgaagat gaagaaaaag tttgtacaat tgatgttcct
    14641 gtaaaagtaa gaaatgtcgt gctaaagcct ttagataaaa gcaagttctt tttagattta
    14701 tggcaacatc caagttgttt agctagaatg tataaagttg agttatggtc tgatatacat
    14761 tttgaaatag tcgataacta tttaaaagaa ttagcatctt taggagaaaa agttgcaaca
    14821 gttatagttt cagattatcc atgggcaggg caaagttgtt ataaagttta taaaaatcca
    14881 tcaaacctat atgaatataa catggtaagt gtatcaaaag gattggatgg aaagattaaa
    14941 tgtaactttg agagtatgga taggtatata agcatagctg ataagtataa aatgtctaag
    15001 gagatagatt tatttggtct attaggtaat tggtgtgctg gtgagtttgg aaatcctgta
    15061 gaaggttata aggaccctat aagagtaaga tattttgatg aaaatgataa agtatttaaa
    15121 tttataaaca atacaaatga tttaaaagaa tatataggat tagtattaaa tcatttaata
    15181 gagtgtggat tatgggatag agtaaggatt atagctgatg aaccaaataa tccagaagta
    15241 gtcaaagagt gtatagaatt tataaattct actgtaggta ctcatcaagt taaatataaa
    15301 tcagcaactc acgaccaaaa ctttttagat agagctaaag atgaaattga tgatatgtca
    15361 ataaatttaa aacttactat acaaaattat aaagatatag aaaatcttaa gaaaaaaatc
    15421 aatgacaagg gtgggatatt gacttggttt gtatgctgtt ttccagagaa accaaatagt
    15481 tttttaagtt ctccttttgt agaaaataga ataataggat ggtatactta ttactttggt
    15541 cttgatggat ttttaagatg ggactataat ttatggacag aagacccttg gaaagattca
    15601 agttacaaat tccctatttg gaaagctgga gatatgttct ttgtttatcc tggaaaagat
    15661 ttaaagccag ttagaagtgt cagaatggag aatttaagat ttggaataca ggactttgag
    15721 ttatttacaa tgttagaaaa agaaaagggc agagagtata tagaagttga gcttatgcaa
    15781 gaattattaa ataaaaaaga aaatgctgaa attaaaggtt ttggtgacat agaattagga
    15841 tattcacttg ataattgggg atatgataaa gttaaaaaat atgtattaga gttgttagac
    15901 agagtataaa aaaatataag agtgtctcaa aatgaacttt ttagttcatg aggcactctt
    15961 tttgtatgaa atcaatatag tttcattgtt tcaacaatga aaaataggat tacttatatt
    16021 ctaagatagc cctttagttt tttagttgta ttcaatgttt taaaactaac tataaacgct
    16081 ataaagttta taaatgacaa taattatatg taacatgatg tactttaatt tgctataata
    16141 gggattgaat aatattatat ggaagataac attgtttggg gggattaata taaaaagaat
    16201 aggtattatt ataatgtttt ttttaagtat agcttttatt ggattttatc ttgcaataag
    16261 aggtaacagt gatacagtta agatgcaacg agggataaat ataggtaatg cactagaatc
    16321 acctaaggat tttccgtggg atgtaaaaat gtcaaataag ttttttgatg atataaaaga
    16381 tgcaggtttt gacacagtac gtataccagt aaggttttct gattatacgt cagattctga
    16441 taattttaaa attgatgaag aattttttaa aaagattgat aaatatgtag attatgcatt
    16501 ggataaagac ttaatagttg tgttggattt acatcatttt gaagaaatta tgaaagaacc
    16561 aagagttcat aaagaagagt ttttaaaaat atggcaacag atagctaata ggtatcaaaa
    16621 atatgataaa aaattggtat ttgaattatt aaatgaacct aaagaaaatt tatcttcaca
    16681 attactaaac gaatacattg aagaagcaat aaagataatt agaaaaacta accctaaaag
    16741 aactattata gtgggaccat ataatttcta tcaaattgat tatttgaatg aattaaatgt
    16801 tccaaaagat tctaatatag tagtttcttt tcactattac gaaccaaatg attttgcatt
    16861 tcaaggaaac atttatcata atggatttga gcacttgagc aatattactt gggaaggaac
    16921 aaatgaacaa atggattatt taaaaaaaag gtttgatatt gtagaaaatt gggcaaataa
    16981 aaataaggta aaaatattct tgggtgaatt tggagtaaca aaagaagcac cagaagcttc
    17041 aagaagagct tggataaaag ctgtaagaga agaagctgaa aaacgaaact tttcatgggc
    17101 ttattgggag cttgcttctg gatttggtat atacaatcaa attgaaggga cttgggatag
    17161 agacatatta agtgcattaa tagaaaagag gtgattttat gaagaaaaat tcaaaaaaga
    17221 tacttataac tttaactatt attactaata tcgtatatat attgtggaga attttttaca
    17281 cagtacctaa agaagaaggg atgtttgcct taatctgtgc tataatactt ctttttgtag
    17341 agataatggg tatgatggaa atgtttgtac attattatgg tatgtcaaat attgagtatc
    17401 ctgaaaaacc agtcataagt gaagaacttt atccacatgt agatgtattc attgcaacat
    17461 ataatgagtc tgttgatttg gtgagaaaaa ctgtaaatgg atgtattcat atgcagtatc
    17521 cagataagaa aaaggtacat atatacatat gtgatgatgg aaatcgtgaa gaaatgcgta
    17581 ttctagcaga aaaaatgggt gtaaattaca taactagaac tgaaagagaa ggagcaaaag
    17641 ctgggaattt aaataatgca atgcaacata caaactctcc tttgattgct acgtttgatg
    17701 cagatatgat accaatgcat gactttttaa ttgctacagt accatacttt ttgaaaaatg
    17761 agcaagctaa aaaagatgga gaaaaagagg agtatgaaaa ggttggtttt gtgcaaactc
    17821 cacagagttt ttataatcca gacttgttcc aatttaatct gcactctgag ggaaggattc
    17881 caaatgagca agattatttt tacagagata ttcaattggc tagaaatagg acaaattctg
    17941 taatttatgg tggtagtaat acagttattt caagggaagc acttgaagaa gttgatggat
    18001 tttatactta ttctattaca gaagattttg caacaggtat cttaattcaa agtaaggggt
    18061 atagatgtta tgctattcca gaagtacatg catcaggttt atctcctaca gatttaaaaa
    18121 gtcttattaa gcaacgtgaa agatgggcac gtggatgtat acaaactggt agacgtctaa
    18181 atattttgtt taggagaggt ttaggttttt ggcagaaaat aagttatatt tcttcaatta
    18241 cttattggta tgcaagtata aaacgctttg tatatataat ggcacctatt ttattttctg
    18301 tgtttaatgt gattgtagta aaatgtacat tacttcaagt attggtattt tggttgccaa
    18361 tgtatatttt aagtagttta tctcttaaaa tattttctca aaatataaga aatactagat
    18421 ggaccaatat atatgaaact ataatgtttc agtctttaat gccagcagta atacttgaaa
    18481 cgtttgcaat atctaaaaat aaattttcag taacaaataa gaataaatta gaagaaaata
    18541 gaatgtataa gtttttacag ggaatacctt attttattta catggtctta tctatcatag
    18601 gaattttaaa aatgtttgta gcaatattta aaatgagttc aatgacatat tcagttgtat
    18661 tattttggtt gattggaaac ctttttaacc ttgttatggc tacattattt atatcaggga
    18721 gacaacagtt aagaaagtct gagcgttata ttgctgaaat tgattttaag ttaaagcaaa
    18781 actcttatgt tctttcttca aaaacgattg atatatcaga aaatgggttt gcatttttgc
    18841 tagaaaatcc agaatatatt tccccagaag aagagttcga ggtagagttt agagaaaaat
    18901 caggaaatga aatgtatata gctaatatga aagcaaagat tgttaatgtt gtggaagtaa
    18961 attcaaagtg gaaatatgca gcttatatta cacatattga agatagtgaa atagataatt
    19021 ggatgtgtat tgttcacgat agaataccta ctttaccgat gacaatatct aatcaactag
    19081 gtttctttga tgatttgcaa attaatgtaa aaaaacgtat tgaaaagatt agaacattat
    19141 cacgaagaag tccaagaatc aatatgaatt ttcagatgga tataaaaaat acaggtaaat
    19201 taagaatagt aaattttaat tatcaatatg tattattaaa ttttgagaat aaaaatattt
    19261 atcctaaaga aatagcattg gaaattaatg aaaatattgt gttagaatgt gatttatgtg
    19321 agggtaaaat cgatgaaaga ggtctcttat ataaagtaaa taacattgac agcataatgc
    19381 aaaatttctt tttaagagat gaaatgatgg attggatatt acaaaataag acaattcctg
    19441 tttcaaatcc tagtgaaaag aaggaaaaaa gtatagatga gtttgaaccc atggaatata
    19501 tttaggagag atgaaatgaa gactttaaaa gtgttttttt gttctgcaaa atatcttatt
    19561 ttggtgttta ttttttttat aaatactact tttataagtt cagcgacaag tgataaaatt
    19621 ttagatttat cctttaaaaa gatagagact gatttaagtt ctaggattac atatgaagat
    19681 acaggtgtaa aaatagaaac agatagttct aaaagtgata aggaacgtta tttatatatt
    19741 taccaaaata taaaagaaaa ttggagtatg tataataatt tttatattga aattcaaaat
    19801 aaaaataaaa gttcccagaa aataaattta agtatacaaa gtaagaatat gcttgaattt
    19861 aggttaaaag aaggtagtga aatatttttg gaagataaaa atataatata ttcagataaa
    19921 ataaaagaag gatgcataga agtaccaggt gaatttgaag gcaaaattta tgtgaacttt
    19981 aatagcctta taaatgagaa aagtaatgtt gtgctggact caagtatgct ttcaaacatt
    20041 gttagttggg gaattacatt tattccatct gatgaagaac ataatattgt tattataaaa
    20101 aagatatctt tattgtctga agagaaatta agttttttac ataatattaa aataactggc
    20161 aatgaagaag tacaaatacc agtattgggt caaagtataa gtcagtatga ggtattaggt
    20221 cttaaacgtg attctaaaat taagtattct cttatgggaa aacaagataa tgtgagcata
    20281 tctcaaaatg gtaaattaac attaagtaat aaagccgaac ctggacaaat tattttacaa
    20341 gttaatgttg atgataagtt taaaattgga aggaaaatag cattaactga atcttggagt
    20401 attaataaaa aagataagga tggtgttcca tatacattgg tatctcctga gcaatcacca
    20461 acagtacaat atatgaaaaa aataaacttt atgaataaca tcataacgtt tgttcgaatt
    20521 ttatttgtat ctctagtaat aatatgcttt ggaatatatt tatattggaa aaaatgttca
    20581 aaaactaagt aaagagagga ggttagattg tgttattttc aagtttaatt tttttatttt
    20641 attttttacc tataactttg gttttatatt atgtatttag gtttaaccga acgatacaga
    20701 acatgatatt gcttgctgtt agtttatttt tttatgcttg gggagagcca aaatttgttg
    20761 ttatcatgat agtttccata ataatgaact atatatttgg tttactagta gatagatata
    20821 gggaaagtaa aataaaagta aaagtttttt tagttttgat gtgtgtatat aatataggtg
    20881 ttttatttat atttaaatat ctagcttttg cgctaagaaa tattagtgaa cttattaata
    20941 ctgaactaac tatacctagt attgttttac ctataggaat ttcatttttt acatttcagg
    21001 gaatgtctta tgttatagat gtatatcgta gacatggaga agtccaaaag aatccttttt
    21061 atgtgggact gtatatagca tttttcccac aattaatagc aggtcctata gttagatatg
    21121 agagtgtggc agaacaaatt ctcaatagaa aagaaacttg gaataaattt tctattggga
    21181 cttgcaggtt tataacaggt ctaggcaaaa aagtattaat ttcaaataac atggctatag
    21241 tagcagacta tatttacact atgaatagtc aaggagaaat agctgcatca cttgcatggc
    21301 ttggttcaat agcctatact atgcaaatat tctttgattt ttctgcttat tcagatatgg
    21361 ctataggttt aggtcttatg tttggtttta aatttgaaga gaactttaat tatccatata
    21421 tatcaaaatc aattagtgag ttttggagaa gatggcacat ttctttggga atgtggttta
    21481 agagctatat atactttccg cttggaggtt ctagagtagc aaataaagat attatgatta
    21541 gaaatatgtt tatagtgtgg ttatttactg gaatatggca tggagcagaa tggacatttg
    21601 taatttgggg gattttaaac tttgttttct tgattataga gcgatttata atgtttgaaa
    21661 agattgaaaa tcacaatttt ataaaacata tttatgcgtt attagtagtt aactttgggt
    21721 gggtattgtt taggtcacct aatttaaaag aagcatataa ttatttcaaa gctatgcttg
    21781 gaggcaatgg agttatatgg agtgattata catatatgtt tttaaaagaa tactgggttt
    21841 tctttatatt tgcatttatt tttagtgtac ctattgcaaa gaaaatcaat aagtttgttg
    21901 ttgaacaagc taaatatagt atgatattta acgtatttta tccaatctca gctattttat
    21961 tgttctttat ttctgtgaca tatttagtaa ctggaagcta taatccattt atttatttta
    22021 atttttagtt gggaggaaaa gtatgaatag attttttaaa gtaagattaa taactgcaat
    22081 tatgttctta attattttat tcgctttctc tatctttaat atttgttatt ctggaaaaga
    22141 tatgatacag gagataaaaa attcagttgc tgaaaaaagt agtttaaaag atacaatatc
    22201 atctattgat aacacagtaa atgacaaagt aatatataaa aatacgttca ttgaaactta
    22261 tggctactta caaaagttaa tgggtaaaaa tgagttttca aaatttagtg ttgtaaaaga
    22321 tacttctgga aagatgcatt atacatattt tgctaaagct ccaaatccag ttggtgtgat
    22381 agcagatagg gtaagaaaat tttctgagga aatggaaaag caaaacacta aattagttta
    22441 cttaatgact ccagataagt atataaaagg agtgacaaaa tttccaaaag gaatacctta
    22501 ttcttatagc aatgagacag cagataattt tttaaatcaa ctaaaaaagt ataatgtgga
    22561 ctatattgat tttagagaaa atattttaaa gagtggtatt ccaaaagatg atttattttt
    22621 taaaacagac catcactgga aagtagaaac ttctttttgg gcatttggag aattagttga
    22681 gcaacttaat aaaaaataca atatgaattt agatgaaaat cattactata gagataaaga
    22741 aaattataac tcaattgtat atcctaagtc atttcttggt tctatgggaa gaaagactgg
    22801 tatcttatat ggtggtatag atgattttac attaatttat cctaaattta agactaatta
    22861 tacttattat acagattcta aatctcaaaa atttgaatta aaaggaaggt ttgaagagag
    22921 tttaatatta tcatatcctt ttaatgcaga ccttgattta atggatggtc aatcagataa
    22981 gtattttaca tatttattgg ggaatagacc attagttaaa ataaaaaata tagaaaatcc
    23041 agatggatta aaagtgctgt ttgttaaaga ttctctaatt gtaccaacag cagcattttt
    23101 ttcctctgtt tgttcatcta tagatatgat agaccctaga tattatgatg gagatatact
    23161 agaatatgca aaatcccatg actatgactt tgtttttata tctgtatatc cccaaaatct
    23221 tacaaaagaa ttttttccgt tttgtaagta aatttctaaa gaaattttct tataaaataa
    23281 aaaataaatg gctaactagt taaaagttag ccatttatct taagtgttac tctaagaaat
    23341 gtttttttta ccaaatactt ctttatctat ttttccaagt gattttccta cagtaattga
    23401 tgataccata gcaccattaa catttatcat ggtacgtccc atatctagta tagggtctat
    23461 tgcaagtata ccaccaacta atggaaaata agaaccaaga ccaactccag agataacaac
    23521 tgaaacggcc attgtagcag ttcctggaag accagctatt ccaaacgaac taattgttat
    23581 aacaattaac aacataacat aaaggctcat atccatgttt actccagcca tattggcaag
    23641 tgttacagcc attaaagcag gatatatacc tgcacaacca ttcataccca tatttgcacc
    23701 taaacttgct gtaaagcttg atacaccctc atcaactccg tgattttgta ctagactttc
    23761 tatcgttaca ggcaatgttc ctaaactaga acgtgaagta aatgccagaa gtaaagcttc
    23821 agttgaattt cttatataag taatgggatt tagaccattt aaagttataa aaattaaatg
    23881 tatggtaaat aatattgcca ctgatatata aagagctaat ataaactgta aaacagatac
    23941 tattgagcta ataccttgtg aagttattgt atttgcaagc agagcaacca cagcataagg
    24001 cataaattta ataacagtca tagacacact tacaattatt ttgtaagatg cttctataaa
    24061 atctataaat ggttttattg tttcaaagtg ttttttactt aatctcttta ttgaggtagc
    24121 tataaatgta gcaaatatta ttgtagctac aatatttcca tttgccatag ccattacagg
    24181 attagatggt agtaatcctc ttaaagtatc cacaagagag tttatttctt taagttcttg
    24241 attttcaact aaagtaggca tattagctcc tacacctaac ttcattatat ttgcaacgat
    24301 aattccaaca attgcagcta tagcagtagt agcaagaagc atacctaaag atttaaatgt
    24361 cagagttcca agattgttat ctttcatatt tataattact tttataatgg ataagaaaac
    24421 taaaggtact actaacattt ttaataagtc cataaatcca gaaccaaata ggctatacca
    24481 cttgcttact tcttgtagcc aagtgacttg agtggtatca ttaggaaaac cagcaaaagc
    24541 ttgtattatg attcccaata tgagtcctag tactgtagca tatattgttc tcaatgaaaa
    24601 ttttattttt ttcttttcaa attgtctaac aataaagaat gtacctagaa gtaatattat
    24661 gaaaaaaatc gttttaaaat ctgtaatcat aagaaattgt gataaaaatg aactttccaa
    24721 tattttttct ccttttattt aaaaataaat ttttctaagt ttaaatgata ccaatatatt
    24781 aaaaaaatgt caatattata tcacaattta cttaaataga gtaaaataaa ttgattttga
    24841 aaaatgtgat taagtataga aaaatagggt ataataaatg tttaaagaat ttgaattgtg
    24901 gtaagaaaat aaaaaatagc ttttataata tttgatttca aattaaaata tgttaaataa
    24961 ataactaaaa attgttatga aaaatgttta catagaatat atatcaaatg aaaagcttaa
    25021 aataagtgaa gaaattaact agacatcaaa ggagagataa aatgagagta gtaataattg
    25081 gtggtgttgc agctggtatg agtgctgcag caaaattaaa aagaataaaa cctgagtatg
    25141 aagttgttgt ttatgaaaaa actgaaatag tatcttttgg tgcttgtgga ttaccatact
    25201 ttgttggtgg tttttttgat gatgctgatg aattattagc aagaacacca gagaaactta
    25261 gagaagctgg aattgattta aatatattta gagaagtcgt tgaggttgat agtgaatcta
    25321 agaaaataaa agtaaaaaat ataaaaacag atgaaatata tgaagattac tatgataaat
    25381 tgatgatagc aactggagca agaagtatta tgcctcctat aaaaaacata aaattgaaga
    25441 atgtttctac attaaaaagt ttatatgatg gagaatatct aaaaaaactt ttaagtaatg
    25501 aagataataa aagagtgaca ataataggag ctggttttat agggttagaa gcagttgagg
    25561 catgtaagaa attaggtaaa gatgtagatg taattcagtt agaagataga atattgccac
    25621 aagtatttga taaggagatg acggatgtat tagaagaaga aataaataga cataatgtta
    25681 atttgcactt ggatgaaatg gttgtagaac tttgtggaga agataaagta gaaaaagtta
    25741 taacaaacaa aggggaaata gatacagatg tagtaataat tgctactggt gtgagaccta
    25801 ataccgagtt tttgtcaaat actaatataa aaatgctaaa aaatggagct atagtagttg
    25861 atgaatatgg aagaacttct gtagaagata tatactctgc tggagattgt gcaactataa
    25921 agaatatagt aagtaatgaa aatgtatatg ttcctttagc tactggagct aataaattag
    25981 gtaggatagt aggagaaaac ctagcaggaa gagaagtatc ttatcaaggt tcactatctt
    26041 caagttgtat aaaaattatg gatatggagg cagcatcaac tggaattaca gaaaagcaag
    26101 ctaaagattt aggcataaat gtcaaatcta aatttatttc tgattataat caaactcatt
    26161 attatccagg aagaaataaa atatatgtaa aacttattta tgatgcagat acaaaagtta
    26221 ttttaggcgg acaagtggct gggtttaagg atgctgtcca aagagcaaat gtattggcag
    26281 ctgctatctt tggaaaaatg accacaagtc aacttggaat gttagattta tgttatgccc
    26341 caccatttgc aagaacatgg gatgttttaa atgtagctgg aaatgtgtct aaataatatt
    26401 tagattatag ctaatatagt tgacaagcaa ttaagggaat gaatttacct aaactatttt
    26461 tatcatggta aaattcatcc ctaaattttt attccaaatt tttagaaata aggattccat
    26521 taaattattc atcaaaattt aaacgcaagc ttaatctatc ttctccaaga atagtatttt
    26581 caaaaacatt agtagcattt tctaaataca tactaggtat gtctaaagaa ggactaaaat
    26641 gggtcagtaa aagttgtttg acatttccaa gttttgcaag attagcagct tctctaaaag
    26701 tcatatgttt gtttctaaca gcttttgata tatctaaatc atcaccatac atggcttcac
    26761 atataaataa atcactatct tttatgaaat ctggtattga ttctataggt cttgtatcgg
    26821 ttgtaagact tatcttaact ccctttctca attctcctaa gaccatatct ggagtgtatt
    26881 gttttgaatt taaaactata ttttgacctt cttggagttt gttccataat attttaggaa
    26941 cttcgttttt agttgcctta tctatatcaa atttagggtt tcttctaaaa ttaaaactgt
    27001 atcctaaaca ctctatagag tgttctaaac tgattgttga aatttctaaa tttcttaaaa
    27061 ctttattatc taaagaaaaa ttgccttgag ggttttctat aatttttaaa gtgtatggta
    27121 catattctac taaatttctc atagaacgta tacaatcgac tattccaact ggaccaacta
    27181 tagttaaatc acttgtacga ccactattcc caattgttga taaaagtcct aaaagtccaa
    27241 atatatggtc accatgtaaa tgggttatac aaatcaagtc tatatcttta aagccacatt
    27301 tttttagctt catagatacc tgagtacctt caccacagtc aattagtatt tttctacctt
    27361 tatagtttat aaatacagag gatagaaatc tgtttggcat aggtacattt ccaccacatc
    27421 caagtaaagt taaatctatc attttttccc ctttctaaaa ttaacacaaa tctgttgttt
    27481 gtttttatta tgatatccta ttataattat tttaacatta aatataacaa taaatgtgta
    27541 tttgagtcaa gaaatattat caaataaagt ttttatatag caatgtataa aaatttggag
    27601 aacgaaaagt gaaaattata tttaggagta tttaaaggag gaaaatttaa tgaaagtatt
    27661 aaagaaaatt actattttgt cattgattac attaacctta gttggttgta gtaacggaga
    27721 agataaaaaa gtggttgaag gttctaagaa aattcaaaat tctaaaaaag atgaacagcc
    27781 tataatagta cataaagatg caaatatatt gaaagatagg tatgagccac caaaaggatt
    27841 taaaagagtt gatgtagaaa aaggtagttt tgaatcattt cttaggaata ctaagctaaa
    27901 aaattatgga gagaaagtaa agtattatga tggaagaatt aaggaatcaa aaggtattta
    27961 tgatagtgta tttgatgttg atattggcaa tagagatttg catcaatgtg cagatgcgat
    28021 tatgcttatg agagcagaat acttgtatca gaataaacta tatgatgaaa taagttttga
    28081 ttttgttgat ggttttaagg cgaagtattc aaaatgggca caaggatata gaatttcagt
    28141 aaaagatagt ggttcaagct ggtataaagc aactgaatat tcaacatctt atgagagttt
    28201 cagaaagttt atggatatag tgtttgcata ttcaggaacg ttatctcttg aaaaagaatt
    28261 ggagcctgta aaagttgagg atatgaaaat aggagatgtg tttatagttg gtggaagtcc
    28321 aggacatgca agtataatta tggatatggc agaaaatgaa aagactggta aaaaggtatt
    28381 tatgatagcc caaagctata tgcctgcaca acaaacgcaa cttcttatta atcgtaaaaa
    28441 tccaaatttg agtccttggt atagtttaga ttttgaaggt gatttgacaa tacctcaatg
    28501 gacttttaaa agagaccagt tgaaaagatt ttaaaataat aattttatat aataaaagaa
    28561 aaatatagat gtgaaaaaat gtaattagat taaaactaat tggatatgga caaaattaaa
    28621 ttgatagata aatattaaaa attacactaa tcaatcttac tatagtttaa tatttatcta
    28681 aaaagtttta tgtaaaatat aaattttata ttaaagctaa aactaatttg aggaggctaa
    28741 tttatattga agattagaag gtatttaatt ttggtaatta taattctaat tacaacaagt
    28801 cagagtttta tttcttatgg gctagagcga tttgaagaaa taaccatttt tcacactaat
    28861 gatatacatg gaagatatgc tgaaggtgat gaccatatac aaataggaaa tctagccaca
    28921 ttgaaaaaag aaactccaaa ctcaatttta gtagatgcag gagattgttt gcatggactt
    28981 ccaatagtaa atatggatag agggaaaagt gcaattgaat taattaaatg tgctggatat
    29041 gactatatta ctcctggaaa tcatgacttt aattatggaa aagatagact gtttgagtta
    29101 tctaaaagtg ctaatttagg agagaatact ttaaaatttt tatcatcaaa tatttttgaa
    29161 aatggaaaaa aagtttttga tagtaatgat ataaaagaaa tagatggaat aaaaatagga
    29221 ttttttggac tctcaaccca ggaaacaaag aacaaaactg gatttaaaaa tgtggaagga
    29281 ttagagttta aaaatcctat agaaagtgca acagaacaag taaatgaatt aaaaggaaaa
    29341 ggtgccaatg taatagttgc aatatgtcat attggtacaa atagtttaag taagccaaat
    29401 agtatagata tagcaaatga ggttcaagga attgatttga ttattgatgg acacagccac
    29461 actaagtttg aaaatggtaa acaagtgggt gatacattga ttgtaagtgc aggacaatat
    29521 ttagaaaata ttggcgagat aaaactaact ttggatacta atgatgtaga aaatataaaa
    29581 atagaaaata aatcagttag attaattgga aaagaagaag cattaaagta taagcttgac
    29641 tacgttgtaa gtaaaaaggt tgatgaaata aaagaacaac aagaaaaaat attagataaa
    29701 gttataggtg caactacaaa tactctagat ggttcatatg aaaatgtaag gacaaaagaa
    29761 actaatttgg gtaatttaat aagtgacatt ttactagata aaactaaggc tgatatttct
    29821 ttatttaatg gagggaatat tcgagatacg atagaaaaag gcaatataac aagaagagat
    29881 attgtagatg tatttccttt tagcaatact atagttacca aagaacttac aggagcacag
    29941 attaaagatg tattggagca tggagtaaaa ctttatccag aaaaaagttc agcctttttg
    30001 caagtgggag gaatttcata ctactttaat cctaaacaaa aagaaggcga aagaataaca
    30061 agtattcaaa aagaaggaaa gccattagat ttaaataaaa agtatgtagt tgcaacgaat
    30121 gattatatag cctcaggtgg agatgaattt ccttgttttt caaaagagcc aatactaaag
    30181 gaatttggaa atttagagag tgcagtcatt gaatatatag agtacaaaaa agaaatatca
    30241 aaaaatgtag atggaagaat tggtattaaa tctaaagatg agttaataag caagaatcat
    30301 gagagtgatt tgatattaag tgaaaatgaa aaaaatatca atgataatga agagaatcta
    30361 tctaataata taaaaagctc tgatagtgac aaaaataaaa gcttagataa aacaagtgct
    30421 agagaaagtt ctaatcaagg aatatctaaa agagaagcta aagaaaaatc tcctaaaaca
    30481 ggagatttag gtttttcaaa tagtataatt atatttatag tttcaagtac attgatatgt
    30541 ttattgaatt ttaatcaaaa agagttaaag gataaaaagt caaaataaaa attaatgagc
    30601 aaaattagta gatagtaatt taattttaga taatagtaaa cagcaaagga aattagttat
    30661 ataaatacta atttcctttg ttttatgctt taacatgatt atttttaaat aaaattgtat
    30721 tttatgaaaa ataattacac taaatggcat agtattatgg ggatttttag tttttattca
    30781 agtaaatgaa atcatattaa caacttctga tatatttaca aattgataaa aaaatgattt
    30841 tattagtatg atataattta gctagtatat atgatgatgg aggatatagg gatgaataat
    30901 atattacttt tggaagatga caaatcttta aatagaggaa ttagttttaa attaaaaaaa
    30961 gaaggataca atgtttttag tgcatttggt atagaagaag caaaaaatat tttcgcaaaa
    31021 gaagagatat gcttaattat ttccgatata ggtctacctg atggtagtgg ttttgacttt
    31081 tgtgaggaga ttagaaagaa aagtgatgtt tacataataa tgttgacagc tcttgatgaa
    31141 gaggtggata ttgtcacagg atatgattta ggtgcagatg attatatcac aaagcctttt
    31201 agcttgatgg tattaatatc aaaagtgaat gcgcttatga aaagagtaaa tacagtaaaa
    31261 aattatactc tattagtttg tgatgattta ttcttttatt atatagaaaa taaattaatt
    31321 ataagaacag atgataagga agaagagata attttaagta agacagagac aaaactatta
    31381 aaatatttga tggaaaattc tatgcagaca ttgactaaag aacaactgtt agaatcttta
    31441 tgggatagta gtggtaattt tgtagatgat aatacgattg cagtaaatat aagaagactt
    31501 agacaaaagg ttgaaaaaaa tccatctgaa cctaaatata taaaaacagt aagaggggtt
    31561 ggatatatgt ggggagaaag gagtataaaa aaatgctaaa aaatatgaac ctcaaagatg
    31621 gtttaagtgt gaaagatgat tatataatga gaagagttat tgtatattct gttttatctg
    31681 tactaatagt aacccttgta tttatacttt ttgaatatag cagattacat aaattgtatg
    31741 aacatcaaat aaaaaatact caaaatattg ctggaatatt gattgaaaaa tatcctaatg
    31801 atgaaataga tatagtaaat tctatttata attccgatta tagtaaggtg tctactggag
    31861 aaaaggcatt taagaaattt ggctatggat tggaaaataa aatgtcaaat gataaaaatt
    31921 ttagtttata cttaaaatct ttttttaagg aatcattttt tattttttta atgatgttta
    31981 tagtagttat aggtataata tattatttta taagatacat aaataaaaga ttgagtaaaa
    32041 tttattttat agtagataag atggcacaag aaaactactt gaaggacact actgagtcag
    32101 aagaaactgg ttatctagta gaatataatg agtattttaa agaaggtaca tttagtaaga
    32161 taaataactg tctatatgag cttaatagaa gtttaaagat aaagtttatt aaattagaaa
    32221 aagaaaaaga aagtgtaaaa tcattagtaa cagatatttc tcatcaatta aaaactcctt
    32281 tagcatcctt aaaattatac aatacattac ttatagaaga aaaattagaa gaagatgaag
    32341 ccaacgagtt cttattaacc aataaggctt ctataaataa attagaaaat ttaataaatt
    32401 ctttagtgaa catatcaagg ttagaaatta gcatgataaa cataaaaaaa gaagataatg
    32461 atataaaaaa tactattcta aatgcagtaa aaagtgttac ccctaaggct aagagcaaaa
    32521 gtataacatt aaatttaaat gaatttaaca gtagaattat accacatgat aaaaagtgga
    32581 ctgaggagag tatttttaat gtattagata atgctgtaaa gtacacacag gtaaatggag
    32641 aaataaatat atctgtagaa gaaactgcta atttctttaa aattataata gaggataatg
    32701 gcatagggat tgaaaaaaat gagtttaata atatatttaa acgtttttat agaggttctg
    32761 ctgatgaaat tgaatctgta gaaggtagtg gggtaggatt gtatttaagt agaaagattt
    32821 tagaagaaca aggtggaaat attatagttt cttcaaaagt agggataggg agtaagtttt
    32881 ctttgtttct tacaactatg taatattaaa tgaaaggaaa atgtaagcct ctacatttat
    32941 tatataagta taaacagctc cagaaataaa aaattaataa aatatggggt aatataactt
    33001 agaatgaggg gataatttta tgaaaatact atatactgaa aatttatcaa agcattatgg
    33061 aaaaggagaa aacttagtaa gagctttgga caatgttaac ttagaaataa atgaaggtga
    33121 atttgttgca ataataggaa aatcaggttc tggaaaaagt acacttttac atatgattgg
    33181 aggtcttgat atacctacat ctgggaaggt gtatatagat aataaaaata tatttacatt
    33241 aaaagaagaa gaactagctg tgtttagaag aagaaaaata gggtttatat ttcaaagcta
    33301 taatttaatt ccatctctta atgtatggga aaatgtagtg ttgccaatcg gtctagatgg
    33361 tagagaagta gatgaatctt ttataaaaga acttttgaaa tcattaggac ttgaaaataa
    33421 acatgacgtt ttaccaaata cattatcagg tggtcaacaa caaagggtgg caatagcaag
    33481 agcattagct acaagaccag ctataatact tgctgatgaa ccaactggaa acctagattc
    33541 taaaacatct gatgaggtta tgagtatatt aaaatctatg tctaagaagt acagtcaaac
    33601 tctagttatg ataactcatg atgacagtat agcccaaatg gctgatagag ttatattcat
    33661 agaagatggc agggtgtcaa aggttggtga taaaaatgat taatactact aaaatagcaa
    33721 aaagtaattt aaaacaaaat aaatcaaaaa gtattctaat aataatcact ataatacttt
    33781 ctactttaat gctttcttct attggaattt atattgttga tgcaggagca tatcaaaaag
    33841 aaaatactat aaagtattca ggtaattatc aaggtatatt agcaaatgta gatgaaaaac
    33901 aggctgatat attgagtaat catgcagatg ttgaattaac tggtgaaatg aatggagtag
    33961 gtgttgaaaa actggaagat gattcaaata tttctctagc atacatgaat gaagatgctt
    34021 tgaaattaaa tagttttgaa cttatcaaag gaaaattgcc aacaaaagag aatgaaattg
    34081 tgttagatag tggagcttta aaggcacttg gttataaaaa taaagactta ggtgaaaaga
    34141 taaaaatatc ttataatgac tataaaaatg ataaaaaaat tgaaaaagag ttcgttataa
    34201 gtggaatctt aaaaactagc gaaatatctg aagcaggtaa atattattat gctattatat
    34261 cagaattata tatgagaaat actagagata tgtcacaaga agattttaat atttatgtaa
    34321 aatttaatga taaaagtaat ttatcaatag agcaagcaaa agaaaaatta gataaaattg
    34381 ctaatgatat aggacttgat actattaata ctgctgtaaa tgaaaattat ataaatgctt
    34441 taaaaccaga tatggaaacg attatgggtg gaggttttgt aggattagta attgtattat
    34501 ctagtatttt agtaatatac aatatatttt atatttcaat agttacaaaa gttcaagaat
    34561 ttggaaaact tagagcaatt ggtgcaacta agaaacaaat aaaaaatata gtgtttaagg
    34621 aagggtttat attagccgga atagcaatac caataggaat aattttaggc tatgtacttg
    34681 caaatattat aataaaatca tttatggata tagatgtaaa atcatcacga ttaccagtta
    34741 tattatcggt tgtggtaata agctttatat ctgttgtttt atcactttta aaaccaatga
    34801 aagtagcatc aaaagtatcc attgttgacg cagtaagata tacaggaaat aaaataagta
    34861 ataagagtaa gagaaaagga tataaaaata taaatctaaa gagattatca catgccaatt
    34921 tagaaagaaa taaaaaacgt acttacatga cattggcatc acttatactt agtggaacta
    34981 tatttataac agtatcaact gctttagaaa gttttgatgc tgagaaaatg gcaagagaac
    35041 actttcctta tgatatagaa gttagactat ctggttatga aatgaacagt gataaaaatc
    35101 ctaaaaataa tttgaatatt ttacaaatgg ataatcctct aagtaaagat ttttttaatc
    35161 aaataaagaa catggaagga attaaaagaa tagaatctgc aagaagtgta aaaattggta
    35221 tggaggacta tgatgttgaa tttaagtatg accttttaca aagtataaat gaaaatgatg
    35281 taaaatcatt aaacaagaat ctaatagatg gaaaaataaa tcttgaaaga ctgcaaactg
    35341 gagatgaaat agtaattact catgttgata cagcaaaaga aatgggagta aaagcaggag
    35401 ataaaataag attaacttta tatgatggag ataaaaagat aaaaaaagaa tttaaagttc
    35461 aagcaatagc tatgggggtt ccaagttttg gtatagggaa ggattttatt gatagaactt
    35521 taaaatacga ttcaactagt agcttgggaa tatatacaga agaaggaaag tatcaagaaa
    35581 tcaaagatag tatcaagaag attgctaaaa ataatggatt tttagagact gattttatag
    35641 atagtagaat tgagtcaaat aaggctgtta tttcatttat aaagataatg ggatacacgc
    35701 taacaggcat aataggtgtg attggattta tgaatcttgt caacacaatg ataactagca
    35761 tagttactag aaaaaaagaa cttggtatgc ttcaagctat tggtcttact aataaacaat
    35821 tagtgaaaat gcttaattca gaggcaatgt attacacttc tggaatgatg ataggaagta
    35881 ttttatttgg aggtatatta gggtatattg ctgttatatt tctaaaaaag actggattat
    35941 cttatgcaac atattctttg ccaatagtac caatcttgtt gatgattgtg tgtattctta
    36001 tagctcaatt tataacaact tatttgatag gacgaagttt taataaagaa tctcttattg
    36061 atagagtaag atacagtgaa taaaatgcaa atatttgtgg ttagtttaac tagcaaaaag
    36121 catataaaaa atatagttaa agagattcat aattaaatag gttaaagcat agtacagagc
    36181 aaacaattta aacggatgtt tgctctgtat tttattatgt aatcaataat atatctaata
    36241 tactgatatt attcatgtta gaaaatgtat cggtacaatt ctaaaaaagt ataaaattgt
    36301 accatataaa atgaaaattt tgtataataa tcgtatagaa aataaatagg agctgatata
    36361 tatgaataag aagattaatt atgcaccagg accaactgaa actagggaaa atgtaagatt
    36421 agcaagagca gaaaaaacaa caaatccaga tatagatatt gattttgttg aattttataa
    36481 aaagacatgc gagaagtttg gcaatatagt tggaacaaaa aacgatgttt acatattgag
    36541 tggtgaagga atattagggc ttgaagcagc atgtgcatca cttaccgaaa aaggagatag
    36601 agtacttgta attgacaatg gtatatatgg agaaggtttt aaagattttg taacaatgta
    36661 tggtggggag tatgtgcttt tttcaagtga atatactaaa agtatagata ttgatgaatt
    36721 gaaagcattt ttagatgagg atagtaattt taaatatgca acagtagtgc attgtgatac
    36781 tccaactggc gtattaaatg atgtaagcaa aatatgtcca ttacttaaag aatatggaat
    36841 attaactgta gttgattctg tagcaggtat ggtaggagaa agattaagtg tggatgaatc
    36901 taaaatagat ataatattag gtggttcaca aaaagctatt tctgcaccag caggacttac
    36961 tatagttgga ataagtcaag atgcaaaaaa ttgtataaaa aatagaaaaa ctgatgtaat
    37021 aggtttttat tgtaatttaa atatatggga aggctattat gaaaaaaaat acttccctta
    37081 tacaatgcca ataagtgaca taatggggct tgataaagca cttgataata tattagaaga
    37141 aggaatagaa aatgtattag atagacatga aaagatagct tatagtgtta gaaaatctat
    37201 tgaggaatat ggtctagaac tatttttaga agaaggatat tctaatacag tcacagctat
    37261 aaaaattcct gaaagtatag gagctttaaa acttactgat tatatgctta aaaattataa
    37321 tacacttgtt gcaacatcac taaatcaata tatggatact attttaagaa taggtcatat
    37381 gggagagaat gcaaatctaa ataaaataga gcatgtacta aatgtcttgg ataaaagttt
    37441 aagtgcatta ggattcaaag gaaatgaaaa tttgttgaat ttatttaata agtattattt
    37501 ttaatagata gggggatata gtatggaagt tacagctaag caaaaattta caacgaaaga
    37561 catggtggaa acctcactat taatagcatt agtatttatt gcaactaaat ttataaatat
    37621 aagattacca atatctataa atggtgggct tgtgcattta ggaacagcaa tgttgttcat
    37681 atcagcaata gtatttggaa gtaaaaaagg tgctttatca ggagctattg gtatgtcact
    37741 ttttgattta atatctgggt ggactttatg ggcaccgttt acctttatag ttagaggagt
    37801 tatgggttat ttacttggaa agattgcatg ggcaaatggt aataatggga ataatttttt
    37861 aatcaatgta ataggaatat gtgtctcatc aatttggatg ttatttggat attatataac
    37921 agaagttata ctttatggta attttatagt acctcttact tcaataccag gtaacttaat
    37981 gcaagtattg ataggtctta taatagcgct tcctatttct aaggtattga aaaaatgtat
    38041 aaaataaata taaagtgaac taaaatttat gatagcataa attatactgt ataatattaa
    38101 gatggagtac ataattaaaa aattttagta tagaggttag ttcacaatgt tatcagttac
    38161 aagatattca atcaaaaatg ttcgtttaaa gccgtgggta aagtttattt ggtattttga
    38221 aacaaaatca aatatactgt taaataataa gttacttcct acagacagta tagatattat
    38281 tcttaatctt tctgatgtta tggaatataa aattgaaaat caagactata ctgctagtaa
    38341 tatgcatttt aatggaataa gggataaaca tggatttatt attcaacatg gaaatatacg
    38401 tgttataggg atttcttttt atccatttgg tctctatcca tttttaaaga taccaatttc
    38461 agagtttaac agacaaattg ttgacttaga agcagtatca gaactatttg ccaaaaaatt
    38521 ggaagagtca ttaaatccaa cacaatcagt agaaaaaatt gttttatatt tagaagaaat
    38581 gttattatcc atattagaag aagatttaat ttcaaataaa tctgtaaagc ttctaaattc
    38641 atttatatat gataatagat atagcaatat aaaagcattt tgtgataata caaatataaa
    38701 tattaaaaca ttggaaagaa tctgtttaaa atatacaggg tatacaccta agattttaaa
    38761 gagaatttac agatttaaaa tggcaagtag tcaacttatt tataattata aagataatga
    38821 actttttgat tttatttatg aaaatgagta ctatgaccaa gcacacttta taaaagaatt
    38881 taaaaaattt tcagggacgt caccaattaa atttattggt gagaataaga cgattaaaga
    38941 aaatataaag tacaactatt tataattata ctgtcgattt tttacaatac ctccaattta
    39001 ttttttgata tgatatagga aaataaattg gaggttttac atatgaaaat tattacagaa
    39061 atagttgagt ttaatgtgga agaaaattta tcaaaagaag agtttataga aattgtaaat
    39121 gacttagaat taaaatttca ttctattcaa tctggtttta ttgatactga gcttttatat
    39181 gatgaaaagt ctcaaaagtg gattatgata caacattggg aatctataga agacctcaag
    39241 agtgcttcta gtaaaatgtt taaagattct tcaactgaga aatttagaaa agctttaaag
    39301 aatcaaactg ttaagatgaa tataatacca caaattaaaa gttggaaatt atagaactga
    39361 atgtaaaaga tagttaaata gtatcaaact aaattagcca aataacattt tgtatttaaa
    39421 ttgttatttg gcttttagtg tttactttaa tatgagtgtt atatactaga aaaaccaaca
    39481 aactgttaga gtgtggatta taataatgtc atatttggga gctaaaaggc ttaatactga
    39541 tttgtcaata tgtcttttta gcatgttgaa caggtatatt gccatgcttt cattatttta
    39601 tttttttgcc ttatactgga ctacatttgc agtttcctaa tatatttggc tcatatttaa
    39661 atgccattat ctaaaagtac tattgctgta cgagaggcaa taggcatttt agaagaagga
    39721 ggagttgagc taaagtatac atcataatct cttaagtttt ctttttttgt gtttcctcca
    39781 aactctacca tatcagtatt taatatcgtt ttgtagtttg tattttgaaa ctttgaaata
    39841 tcaactgatt gtattagatt agggtggaaa ttaaatataa ataaatattt gcccttactg
    39901 tacaccaaaa tctttttatc attatccata tgtataagtt catgactatt ttggataaaa
    39961 atatcactgt attttgtaag ttcgagcata gatttatcaa aatttaataa ttgtttatac
    40021 tttaagttgt tatcttctga aaggctccat tgtcttctgg catacttata gctccaattg
    40081 ttgccttctc gtggaaaatc aatccactca ggatgtccaa attcatttcc cataaaattt
    40141 agatatcctt cacctgctag agaaaaagtt attaatttaa ttaatttaat aagagatatt
    40201 gctctattga taacatggtt atttgagttt atttccatat tccagtacat ttccttgtca
    40261 gctagccaaa aaataattgt tttatcgcca actaatgctt ggtcatggga ttcacaatat
    40321 cctatatttt tttctccagg tcgtctagta gtaagttcat accacatttt tcccaaatcc
    40381 cagtcttcat cagataagtt agagatagtt tttatccaaa aatcaggtac tcccattgcg
    40441 agtctatagt caaatccaat tccgccgtct tttataggga tacacatacc aggcattcca
    40501 ctcatatctt ctgcaatact tatcgaatta ggtttaattt cttttattaa ttcatttgca
    40561 aattggagat atgttatagc ttcaatatct gtattcatac taaaatattt ttcataacta
    40621 tcaaatgaaa ctccaagacc atggttatga tataacattg aagtcacacc atcaaaccta
    40681 aagccatcaa aatggtactc atttaaccaa aattttatat ttgaaagcaa aaaatgtatt
    40741 acttcaggtt taccatagtt aaaaagtttt gtaccccaag caggatgatt tccctttgaa
    40801 cctgagtgga aaaattgatg ttcacttcca tcaaactcat ttattccttc taaagtattc
    40861 tttacagcat gagaatgaac taaatccaat aaaacagcaa tccccattga atgagcagtg
    40921 tttatgaggt tttttaagtc ttcaggagta ccaaacctag atgagattgc ataaaagttt
    40981 gaaacttggt atccaaaaga tgcataatat ggatgttcca taatagccat aagctgaatg
    41041 gtattgtatc cagctttttt tatcttagga agtatttttt cagtaaattc attataagtt
    41101 ccaatagatt cgctttcagt agacatacct atatgacatt catagattag tggagaagtt
    41161 atgtttttta agtcaaaatt attgtcagtc cagataaatg gagtttttgg ttgccatatt
    41221 tgtccattaa atccaccaaa atctttttga attactcttt ttatatataa tggtatacgg
    41281 tcaaatgttt ttccatttgc agtaacttgt acctttacct ctgatttgtg aggtaagcta
    41341 ttttgaccag gaataaatat ttcccaatta ccagacccaa tttttcttag aggatgagat
    41401 tttctattcc aattattaaa gtcaccaatt agtgacaaac tatctgcatt tggagcccat
    41461 tccctataga agaatccatc atttgtctga tgaaatccat agtaaagatg agcatttgca
    41521 aaagaactaa aatctttata gtttttcaat gtttttgatt tcatatcctc ataatgtttc
    41581 attcgctttt ttatatcaga ttcaaatggt tgtaggtata catcaatatc taaaatttta
    41641 aatctttttt gacttttcat tgtatttctc ctccataata aatattacat ttaaaaaata
    41701 atatttatct catactatct tctctgtata attgatatat attatataat cctaataaaa
    41761 agcttaagcc accaatgatt aatttcataa tttacctccc tagtatttat acttatatat
    41821 cgtattcatc aaaatttaaa agtttgttta aaactctttt aataatatca taatcaaagc
    41881 ctttatagga aagatgttga gaaatttttt gatatatttt ctttttatct tctttcttta
    41941 ctctttcata cctttttttt gctagataca tagcattttc aaattcagtg tccttgtcta
    42001 attcagatac tgcttcattg attgtacttc tattgatacc tttattatat aaattctgct
    42061 taattctgtt tttgccacat ttatttaggt taacatttgt gttaacaatt ttttgagcta
    42121 atacactgtc atttacgaga ttataacttt taagaaaatc tatgaccctc tcaattatat
    42181 tttcatcaaa ttctgatgat agtttttctt ttaatttttt ttcagattgg tcagcatgag
    42241 ataaaatatt taatgcttta tttttagctt ttatatacat ttcatcatct aaaataggtt
    42301 ttaattcttc ttcatttatt tcattccctt tttttaaatt aaatgtataa acaagttctt
    42361 taaatatagc aataaaaaat ttatcatcta cataaatatt aactctatca tcatttcttt
    42421 tttgttgttc tatttttgta attata
//
LOCUS       DAMJVX010000016        40767 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMJVX010000016 DAMJVX010000000
VERSION     DAMJVX010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40767
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.017"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            152..811
                     /gene="cprR"
                     /locus_tag="PC407_000810"
     CDS             152..811
                     /gene="cprR"
                     /locus_tag="PC407_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HDF2496271.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            1022..2080
                     /locus_tag="PC407_000811"
     CDS             1022..2080
                     /locus_tag="PC407_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2496272.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            2331..4475
                     /gene="fdhF"
                     /locus_tag="PC407_000812"
     CDS             2331..4475
                     /gene="fdhF"
                     /locus_tag="PC407_000812"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2745..2747,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDF2496273.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKTWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            4524..5357
                     /gene="fdhD"
                     /locus_tag="PC407_000813"
     CDS             4524..5357
                     /gene="fdhD"
                     /locus_tag="PC407_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDF2496274.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCASVKSNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            5368..5541
                     /locus_tag="PC407_000814"
     CDS             5368..5541
                     /locus_tag="PC407_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496275.1"
                     /translation="MCTKKEEQKCSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            5599..6150
                     /locus_tag="PC407_000815"
     CDS             5599..6150
                     /locus_tag="PC407_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2496276.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            6175..7551
                     /locus_tag="PC407_000816"
     CDS             6175..7551
                     /locus_tag="PC407_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2496277.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            7551..8066
                     /locus_tag="PC407_000817"
     CDS             7551..8066
                     /locus_tag="PC407_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2496278.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(8144..9427)
                     /locus_tag="PC407_000818"
     CDS             complement(8144..9427)
                     /locus_tag="PC407_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HDF2496279.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSHSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            9615..10037
                     /locus_tag="PC407_000819"
     CDS             9615..10037
                     /locus_tag="PC407_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HDF2496280.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSEKNGENIIRDILDGNISSECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            10139..11077
                     /locus_tag="PC407_000820"
     CDS             10139..11077
                     /locus_tag="PC407_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDF2496281.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            11263..13287
                     /gene="ligA"
                     /locus_tag="PC407_000821"
     CDS             11263..13287
                     /gene="ligA"
                     /locus_tag="PC407_000821"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDF2496282.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            13447..14796
                     /gene="rph"
                     /locus_tag="PC407_000822"
     CDS             13447..14796
                     /gene="rph"
                     /locus_tag="PC407_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDF2496283.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEIADEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARAIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            14796..15266
                     /locus_tag="PC407_000823"
     CDS             14796..15266
                     /locus_tag="PC407_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2496284.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            15460..16746
                     /locus_tag="PC407_000824"
     CDS             15460..16746
                     /locus_tag="PC407_000824"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDF2496285.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            16895..17479
                     /gene="clpP"
                     /locus_tag="PC407_000825"
     CDS             16895..17479
                     /gene="clpP"
                     /locus_tag="PC407_000825"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2496286.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            17498..18748
                     /gene="clpX"
                     /locus_tag="PC407_000826"
     CDS             17498..18748
                     /gene="clpX"
                     /locus_tag="PC407_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDF2496287.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(18819..19346)
                     /locus_tag="PC407_000827"
     CDS             complement(18819..19346)
                     /locus_tag="PC407_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2496288.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(19334..19846)
                     /locus_tag="PC407_000828"
     CDS             complement(19334..19846)
                     /locus_tag="PC407_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2496289.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            20026..22389
                     /gene="lon"
                     /locus_tag="PC407_000829"
     CDS             20026..22389
                     /gene="lon"
                     /locus_tag="PC407_000829"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDF2496290.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFNSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     ARKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTAVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            22379..22990
                     /locus_tag="PC407_000830"
     CDS             22379..22990
                     /locus_tag="PC407_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDF2496291.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(23255..24625)
                     /locus_tag="PC407_000831"
     CDS             complement(23255..24625)
                     /locus_tag="PC407_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2496292.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYALGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDISGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(24800..24915)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            25203..26282
                     /locus_tag="PC407_000832"
     CDS             25203..26282
                     /locus_tag="PC407_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2496293.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKATEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            26674..27354
                     /locus_tag="PC407_000833"
     CDS             26674..27354
                     /locus_tag="PC407_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2496294.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            27347..28726
                     /locus_tag="PC407_000834"
     CDS             27347..28726
                     /locus_tag="PC407_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2496295.1"
                     /translation="MINIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGVLLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            28897..29982
                     /locus_tag="PC407_000835"
     CDS             28897..29982
                     /locus_tag="PC407_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2496296.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            30036..30395
                     /locus_tag="PC407_000836"
     CDS             30036..30395
                     /locus_tag="PC407_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDF2496297.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            30448..31320
                     /locus_tag="PC407_000837"
     CDS             30448..31320
                     /locus_tag="PC407_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HDF2496298.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            31321..31764
                     /locus_tag="PC407_000838"
     CDS             31321..31764
                     /locus_tag="PC407_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496299.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYINQNKLVDKSKHTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            31779..32363
                     /locus_tag="PC407_000839"
     CDS             31779..32363
                     /locus_tag="PC407_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496300.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            32616..33653
                     /locus_tag="PC407_000840"
     CDS             32616..33653
                     /locus_tag="PC407_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2496301.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            33734..34099
                     /locus_tag="PC407_000841"
     CDS             33734..34099
                     /locus_tag="PC407_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496302.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINVAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            34104..34421
                     /locus_tag="PC407_000842"
     CDS             34104..34421
                     /locus_tag="PC407_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496303.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            34469..36073
                     /locus_tag="PC407_000843"
     CDS             34469..36073
                     /locus_tag="PC407_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496304.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVVLKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYINVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            36197..37825
                     /locus_tag="PC407_000844"
     CDS             36197..37825
                     /locus_tag="PC407_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2496305.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIAKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            38090..39439
                     /locus_tag="PC407_000845"
     CDS             38090..39439
                     /locus_tag="PC407_000845"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDF2496306.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            39595..40674
                     /locus_tag="PC407_000846"
     CDS             39595..40674
                     /locus_tag="PC407_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2496307.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
BASE COUNT        16754 a         4315 c         7348 g        12350 t
ORIGIN      
        1 aaagtttttg tatctaaaat attttatttg taaaatagta attaattatt gtaagctata
       61 atatgagagt attatggtta atgtgaattt taaagatatt attaaagata taaattagaa
      121 tactattaaa cggataaatg gaggagttat tatgtctaag atattagtag tagatgatga
      181 gttagatatg ttaaagttga ttgaaaatgt gttgaaaaga gatggacatg aagtaaaggt
      241 aattagtgat gtagaaaagg ctatgaacat ggattttaat tattttaatc taataatatt
      301 agatgttatg atgcctaaga tggatggttt tgaagtatgt aaaactataa gaaataaggt
      361 tgattgtcca atactgtttt tgacagcaaa aaatatggaa agtgatatta tgtacggtct
      421 tggcataggt gcagatgact atataaccaa accttttggt gtaggagaat taagggctag
      481 gataactgct catttaaggc gagaaagtag agaaaaaaag aatttattaa ctatctcaaa
      541 tgttcaattt aactttttag gcaaagaagt ttcaattgat ggtaaaaaaa ttaattttac
      601 taaaagtgaa tatctaatat gtgaatttct agcaaaaaat aagggacaag tattttcaaa
      661 agaaagaata tatgagtcgg tatatggttt tgatggagaa agtgatataa gtactatcac
      721 agagcatata aaaaatatac gaagcaaatt aaaacaatat gatatttata caatagaaac
      781 tgtatggggg atagggtata aatgggtgta ataagtataa gtgttgctta gtgaaaatat
      841 tatatatatc tacaaaatgt ttgtaaaata ttaatctata tattaaaatt taatttatag
      901 attaatattt tttgtaaatg acaaaattgt aaggtatttt gttataaaat attaagattc
      961 atagtgtata ctaataatat agtatttagt ataaatcttg agcaaaggat ggttatttct
     1021 aatggataga ctaaagaata ttagaaagag tttactacag tatacatttt tatttttgtt
     1081 aataggtata acaatttacc tagtgtttaa aacattggat attaaaatgc tatcaaatgt
     1141 tatggttatg gtagataaga agtttttatt cataggtgct ttggcaataa tgtgtcacat
     1201 aatgcttgaa ggagtagtaa tgaagataat tatagaaagc actcacaaag ttaatattag
     1261 atttattggt tttaagctag ccattatggg attttattat aatttaataa ctccttttgc
     1321 ttcaggaagc caaccagtac agatatatgt acttaaaaaa tgtaaaatgc cacttagtaa
     1381 ggcaagtgca gttgttacca ataagtctat tattttccaa atagtggtta ctttttattg
     1441 tacaatactt gtattaatga attttgctat gttagaaaaa caaatgaaaa tgattatgcc
     1501 tctagtgttt ctagggatag ccattaattc atttgtctta attatgataa tattagttat
     1561 tttaaaccct aaaaaaataa aatattttat gagaattact ataaagcatt tatcaaaaat
     1621 taagttttta aaatttttag ataataagat tgaatccata gaaagtttta tagatgatta
     1681 tagtaaagca ataaattttt ttgcaaagaa taaaaaggta ttaatttcaa ctataatagt
     1741 aacttttatt caattaagtg cttactttag tgtttcattt tggatatata aagcatttaa
     1801 tttacaaggt tatagctata tttatatact gacattacaa gcatttttat atatggcaat
     1861 atctccaata ccaacacctg ggaatgtagg agctaatgaa ttagcctttt tcactatatt
     1921 caaatcagta ttccctcaac ctttgatggg atatgcagta tttttatatg gtggatttat
     1981 gtactattta attctgattg gaagtggaat atttactgta attactcact atagaatgaa
     2041 aaatagaatt ggcagaaata ttgttttaag tgaaaactaa ataagtttaa agaatatata
     2101 ttgagtatat taaaaaatat gtcataagaa ctagaatatt ttaattaaat ggttgaaata
     2161 taaagattat tatgacatat tttttttgaa atttaagagg aattgtgaga aaattgttga
     2221 atttaataga tagtattgca aaatatttat gattttgcat attataaaaa cactcattat
     2281 ctttaagata aattttactt atcaggggat aaatctaagg ggggaattgt atggagaaaa
     2341 aaattttaac agtttgtcca tactgtggag ctggatgcca attatatcta gtagtaaaag
     2401 ataataagat attaagagct gaaccggcta atggtagaac aaatcaagaa agtttgtgtt
     2461 taaaaggtcg ttatggatgg gattttctaa atgaccctaa aattctaaca cctaggctaa
     2521 aaaagccaat gataagaaaa caaggtgtac tagaagaggt tgaatgggat gaggctataa
     2581 actatacagc ttcaagacta aatgaaatta aagaaaaata tggacctgat tctattatgg
     2641 gaacaggttc tgcaagagga cctggtaatg aagcaaacta tataatgcaa aaatttatga
     2701 gagctacaat aggaactaat aatgttgacc attgtgctcg tgtatgacat gcaccatctg
     2761 tagccggtct ggcttactca ttaggtagtg gtgcaatgtc taattctata ccagagatag
     2821 aaaatgcaga tgttttattt atatttggat ataatggagc agattcacat ccaatagtgg
     2881 caaatagaat agtaaaagct aaaaaaaatg gtgctaaatt aattgttact gaccctagag
     2941 taactgaatc tgcaagaatt gctgatatac atctacctat aaaaggtgga acaaacatgg
     3001 ttttagtaaa tgcttttgga aatgtattaa tagaagaagg tctttacaat aaagaatttg
     3061 ttcaaaatca tacacaagga tttgatgagt ataaagaaat tgttaaacca tatacagcta
     3121 aatatgctga aaagattaca ggaatcccag aagagcttat aagaaaagct atgagagaat
     3181 atgcaaaagg gaaaaaagct atgatacttt atggaatggg tgtctgtcaa tttggtcaag
     3241 ctgttgatgt agttaaggga cttgcatcaa ttgctctact tacaggtaat tttggtagag
     3301 aaagcgttgg aataggacct gtacgtggac aaaataatgt tcaaggagct tgtgatatgg
     3361 gagctcttcc taatgtatat cctggctatc aaaatgtaac tgatgataag ataagagaaa
     3421 agtttgaaaa aacttggggg gtaaaattat ctcctaataa tggttatagt ttaactcaag
     3481 ttccagactt agtactaaaa gagaaaaagc ttaaagctta ttatatattt ggagaagacc
     3541 cagtgcaaag tgacccagat gcatcagaag ttagagaagc attagatgaa ttggaatttg
     3601 tcattgttca agatatattt atgaataaga cagcacttca cgctgatgtt atacttccag
     3661 ctacttcttg gggagaacat gagggagtat atacatgtgc agatagagga ttccaattga
     3721 tgagaaaagc tatagagcca caaggagatg taaaaccaga ctggcaaatt ataagcgaaa
     3781 tttctacagc aatgggatat cctatgaatt ataaaaatac taaggaaata tgggacgaac
     3841 ttagacaatt atgtccaagt ttcttaggtg ctacttatga aaagatagaa actcaaggat
     3901 gcgttcaatg gccttgtaag agtgaaagta tggaagataa aggaactatg tatctgtatg
     3961 aaggtcaaaa gttttctact cctaatggta aaggtaattt gttcgctgct gaatggagac
     4021 cccctatgga agttgaagat gatgaatacc catttagttt atgtactgta agagaagttg
     4081 gacactattc agtaagaaca atgactggta attgtagaac acttagttca ttagaagatg
     4141 aaccaggacg tgttcaaata aattctaatg atgctgaaaa attaggaatt gaagatgatg
     4201 aattagttag aattagttca agaagaggaa gtgtaataac aagagcaaca gttactgata
     4261 gagttaagga aggtgcaaca tatatgactt atcagtggtg ggttggagct tgcaatgaac
     4321 ttacaatagc aaatttagac cctatatcta aaacaccaga gtataaatat tgtgctgtta
     4381 aacttgaaaa acttgaagac caagaattag cagaaaaatg tgtaagagaa gagtatcaaa
     4441 gtttgaaaga caaaatgaca gctacaaata tctaatgatt tattagattt aagatttata
     4501 aaaggcagaa aggctaatat tttatgaaca atatatactc aaaaactaaa ttacaagaaa
     4561 atagtaagga taaagttttt gaaaatagtt atagtctagt ggactacaaa tatttaaatt
     4621 cttttaatgt agaagtaagc aaaataaatg aacatgaagt atcaatagat aatgaagaaa
     4681 taattgcaga atatccactt agttttattt taaatgataa atatgtaaat acatttttat
     4741 gtaccccata taatttagag gaacttatag taggattctt gaaaacaaaa ggatacatag
     4801 aaaacaaaaa aagcttatta aaatttgaga taaatgagag tgaacgtttt gcaaaagtaa
     4861 ctattcaaga gaaacaaaaa aatcaagatg agcaaattat atttttaaat tcactagact
     4921 atataacatg tgcttctgta aagagcaata taaaaataaa ttatgatacg atttacagta
     4981 taatggatag aaatttaaat tcatcagaat tatttaaaaa tactggtggg gtacatagcg
     5041 tatctgtatt taattttgat aaaaaaatag tcacttgtga agatgtagca aggcataatg
     5101 caatggataa ggctattgga ttttgtgttt taaatgacat aacattaaaa gataaaattg
     5161 tggctgtaag tggaagaata tcattggaaa tgatattaaa agcagctatg actcaaattc
     5221 ctattgtagt atcaaaatca gcaccgacta atttatcaat agagctttct aaaaaattaa
     5281 atataacgct tattggattt gtaagaggaa gacggatgaa tatttatgca aatccacaga
     5341 gagttatcaa aaagtgagaa taagtatatg tgtactaaaa aagaagaaca aaaatgttca
     5401 aagtgtgaaa tctgtggagt attaattgac gatactgata aggtgtataa taacacatac
     5461 aatttgtgca aaaaatgtaa acgaaatata agtactgaga aaatagtgaa atatataaat
     5521 atatatgatg aaagtaagta gacatatgat gaaagtaact agagggttgg aaatatacaa
     5581 attgaaaggt gggataatat gaaaaataaa ataggctctt ttgtaatagc ggacccaaac
     5641 aaatgtacag gttgtagagc ttgtgaagta gcttgtttta caatgcataa tcagcataat
     5701 aatgtgggtt atacagttgg aacagttgat atacctgtta tacctagact ttatttagta
     5761 aaaggtgaca atttctgtat gccaatacag tgtagacatt gtgaagatgc tccttgttta
     5821 aactcatgtc cacaaaaagc tatagtcaaa gaaaacaata ttatgtctgt aaatgaagaa
     5881 aagtgtatag gatgtaaaac ttgtctgcta gcatgtcctt ttggagctat agacttactt
     5941 ccacaatatg aagatggtaa agaagtagaa caagtaattt tagatgaaaa taagaagata
     6001 gcttataaat gtgacttatg taaagacaat gaaaaaatag cttgtataag tgcatgtcct
     6061 caggaagcat taaaacttgt tactccaata gatgacaaaa aagctaagaa tagacaagct
     6121 gcacttagtt tattgttaac aaataaataa aggatacagg ggtaggtgac gtttatgata
     6181 ataaatatag ataaggattt atgtactgga tgtagagaat gttcaaaggt ctgtccagta
     6241 aatgctattg aaggtgaaga gggaaagcca caagagataa atttagatag atgtgttatg
     6301 tgcggtcaat gtgttcaaac ttgtaaatct tatgcctcag ttatagatga aggatttgag
     6361 tttttacaag agaaaaaaca agaaagagag ataccagagt ctataaatga acctatattt
     6421 gcagcataca atgtatgtga tatagataag gtaaagaaag ctttaagtga cccaaatgta
     6481 ttcactatgg tccaatgtgc accagcagtt agagttgctt taggagaaga tttcggatat
     6541 tatttaggat acttaggtgc tggaaaaatg gcagcagcac ttagagcttt aaattttgat
     6601 agagtctacg atactaattt tgcagcagat ttaactataa tggaagaagg tagtgaatta
     6661 attaaaagag tgacagaagg tggaactctt ccaatgttta catcttgttg tccagcttgg
     6721 gtaaaattca tggaaaaaaa ttatcctaaa ttaactaatc atttatcttc ttgtaaatca
     6781 cctcagcaga tgggtggtgc aatatttaaa acttatggtg cagagataaa taatgtagat
     6841 gcatcaaaga tatttagtgt ttctataatg ccatgtacat gtaaaaaata tgaatgtgat
     6901 agagaagaaa tggattctag tggatataga gatgtagatg tagtattaac tactagagaa
     6961 ctagcttatt taataaaaga catgggaatt gattttaaaa atttaagaga agaaaaattt
     7021 gatagtcctt taggtagtta tagtggagct ggaactatct ttggtgttac tggtggggtt
     7081 atggaagctg ctataagaac aggatatgag cttataactg gagaaagtat tcctgatgta
     7141 gaagtaaaac aagtaagagg agaaaatggt tttagaaagt caactattaa aataggagac
     7201 ttagacttaa gagttggagt agtaagtgga ttaaaaaatg taataccagt gcttgaagac
     7261 ttggaaaaag gaaaattaga tgtagatttt attgaagtta tgacttgtcc tgttgggtgt
     7321 gtaagtggtg gaggtcaacc aaaaatttta ctagaagaat ataaggaatt ggcttatgaa
     7381 aatagaacaa aagcaacata cgtgcatgat gaacatttag caattagaaa atctcatgag
     7441 aatcctgata ttaaaaaact gtatgatgaa tatctggtag aaccattagg agaaaaatct
     7501 catcatctat tacacactac atattgtata ggaaaggaag tggctaagtg atgaactatt
     7561 ttgttatagc agaccctgat aaatgcatag gatgtagaac ttgtatgata ggatgtgtag
     7621 ttgcacattc agatgaagat attttttacc aaaatccaga tgaaataaat tttaatccaa
     7681 aactttctgt cataaagaca aaagaagtaa gtgctccaat acagtgtaga cattgtgaag
     7741 atgctccttg tgcaaaggca tgtccaaatg gtggtatagt tagggttggg aacacaataa
     7801 aaataaatga agaaaattgt ataggatgta agacttgtat gttagcatgt cctataggtg
     7861 caatagacat agttacatta aaagatgtag atgaaggaaa gctttgcttt agagaaagaa
     7921 tgacagcaaa taagtgtgat ttttgtatag aatcgccaga agggcctgca tgtgtaaatg
     7981 tttgtccaac taaggcattt actatagtta aggatgagga tatagatagt aaagttaaaa
     8041 ataagagaaa attagctata ttgtaatata aaaaagatgg agttttattt atgattaaaa
     8101 gctccatctt ttttattata atatttatat atctaaaaat caattattgc attagtttat
     8161 ctaatctttc tttattcaat tttttcattc ctatagaagt tataataagt aaagtatagg
     8221 caattataac tgataaaatt ataatagacc aagaaaatct gtatccaaaa ttatcaacta
     8281 acaatccaaa taatgttgaa cctaatgcaa atcccactgc aaaaattaat tgtacgattc
     8341 catatatgct accaaactcc ttttgaccaa agaaagagcc agttaaataa gcaggaccaa
     8401 tcatataagc aaatacagaa agacctttca aaatggcaaa tacaaatgca aaagtagaag
     8461 atttatctac caatagtaat gataaagttg ctataagaac caatacaaaa gatataataa
     8521 gtggctttgt ttgaccgaat ttatcaaaca ataatcctcc aaataaatta ccaatgagag
     8581 caaataaagc aaatgtggag cctactgttc ctattagaat aggatttaga taatttctaa
     8641 gataagttgg atattgtacg gataatgatg aaacatatat accaacaaat ataaatccaa
     8701 tagtcataat ccaaaaatat ttaatttttt tagcttcttt taatgtataa ccccatactg
     8761 aaggagaaat tgtatcagat gctttctctt ttttaccttt agattttacc ttttcattaa
     8821 tatctttagg catcctgagt atgaataata ttacaggtat accaactatt aatgctataa
     8881 ttccaaatat tagataagaa tgactgtatc cttgagttgc taataagcga actgcaagtg
     8941 gttgtagaaa tatactacca atagaaccac cagcaaaagc aaagcccaaa gcttttcctt
     9001 tgctttcttc atcaaaccaa gcacttatta aaacaggtat tcctatagca gatataattg
     9061 tagcgccaat ttgaacaata catgatacag tgtaaaattg ccatagttca gtacatatag
     9121 aaaaagtagc aaaagcaata ctagaaatta tacaacctaa taaatatagt atcttaatat
     9181 tgactttaga aaataataca cctataaaag gtgatgcaat tgctgaggca atagtaccaa
     9241 tagtgaatat tagagaaaaa gaagctaaac taaagccttc tccttctaca acataactta
     9301 taaattgagg ctgtatactt gctacaatac taaatggaac agcttgtatt aacatacaac
     9361 caagtactat aacccaagca tttgaaaagt ttttaatgct aaatttattg tttagtaatg
     9421 tattcatggt atctctcctt taggttatga tttgtagata ttaatctaat tattctaatt
     9481 ttatatatta gttttggata gcctaactat ttaaatgtaa tttgggtatt aatataaaaa
     9541 ttaactataa aaattgatta ttttaaatgt atggggtaaa ttaaagtata taaataaatg
     9601 aaaggatgga taaaatgtca tttgaaaaaa taaataataa taacctagat aaaatacaca
     9661 ataggaagtg tattatgctt gtaaacttta ataaaaaaga attatcatta attaaaaacg
     9721 taagtaactt tgctggaata agagaccata taatcttaag tgaaaaaaac ggagagaata
     9781 taataagaga cattttagat ggaaatatat caagtgaatg tgaaaatggg attaagagta
     9841 agtctgtaat atttaataac actccaagca cacaagtaaa tgcattttta gaaggtctaa
     9901 aaaaagtgag aataaataga ccttttacag ctatggtaac agagacatct attgattgga
     9961 cattgaataa tttaatagaa aatttagttc aagaacgtaa tgctatgtct ttaggaaaaa
    10021 caattgaaca taagtaaatt taagatatct tatagtaatt ttaactaatg tacattgagt
    10081 atcatattat attttagaat ataaaatttt aaatgtgggt agatagagga gatttgagat
    10141 gaaagtttta tttacaataa attatggaaa agaaaaattt gataaaataa gagaattagg
    10201 ctatgatgtc atacattact ctgaagatag tattgaaaat aatgaggatg taaatacagc
    10261 agatgtacta gttacataca atccatttga gagattagat atttctaaga tgaaaaattt
    10321 acaatatata cagacaacta gtgtaggtat agaccaactt ccaaaagaag aggtattgaa
    10381 gagaaatatt aagatagcta ataatagagg cggatatagt gtacctattg gtgaaagtat
    10441 tgtattgtat attttagaaa tatataaaaa tagtgcaaaa ttttttaaac aacaacaaaa
    10501 taaagaatgg aagatggatt tttctgtatc agaattgtat ggacaaagag ttggattttt
    10561 aggaacaggt actatatctt cagaagcagc aaaacgatta aaagcttttg gtgtagaaat
    10621 atggggtgtc aatactgatg gaagtagtaa aaaatacttt gataagtgtt ttgctactga
    10681 agatatgaat attgtatttg aagaatgtga tatagtagtt gtagcaatgc cttctacaaa
    10741 aagtacagat ggaatagtaa acaaagatat gtttaaactt atgaaagatg gttctgtatt
    10801 tataaatgta ggtagaggaa atacagttaa tcagaaagat ttagaagaat gtgtagagaa
    10861 atttagaggg gtagcactag acgtatttga aagtgaacct ttaaacaagg acaataagtt
    10921 gtgggagtgc gaaaatgtca tagtaactcc tcataattct tgggtgtcag ataaaaacaa
    10981 agagagaaca tttaatatgg tttataataa tttgaaacac tatataatgg agaataaacc
    11041 tgtagataat gttgtagaca tatcaagagg ctattaaaaa taaaaaattt tgataaaaat
    11101 aaaaactctt attagtcgta ttaaagatac taataagagt tttttttgta ataaatttaa
    11161 agtcaaaaaa gtgatatact aaaaaatata taaataggag ataattaatt atagggaaat
    11221 ttcaaaataa tttaatttta aaaatatgaa gaggtgcaaa aagtgagcgt aaaagatgaa
    11281 attaataaac ttatagagac aataaactac cataatgaaa ggtattataa tcaagatagc
    11341 ccagaaatat cagattatga atacgataag ttaatgaagg agttaatttc tttagaagaa
    11401 gaacatccag aactaaagag aattgattct ccaacaaata gggtaggagg taagccatta
    11461 gataaattca atcaaattgt acataaaact cctatgttga gtttatcaaa tgctttttca
    11521 gagcaagatt tgagagattt tgataagaga gttcaagaat atgtgggaga aaatgttgaa
    11581 tatgttgttg aatttaaaat agatggtctt tctgtaggtc taacttataa caatggagag
    11641 tttgaaaaag gagcaactag aggagatgga gttgtaggag aagatatttc tcaaaactta
    11701 atgacagtaa aaagcatacc tcttaagata aatgataaaa aagaactaat agttagagga
    11761 gaagtatata tatcaaaaga taattttaga aaagtaaatg aacaacaaga agaacaagaa
    11821 cagccattat ttgctaatcc aaggaatctt gcagctggtt cattgagaca attagaccca
    11881 aaactaacag caaagagacc attagatata tttgtattta atatggaaaa tatagaagaa
    11941 tatgatttag aaagtcacag tgaatcacta gagtacttag aaaaactagg attttcagta
    12001 agtcagagtt ataaagtttg taaaagtata gatgaagtta ttgagcatat tgaatactgg
    12061 acagataaca ggggtaattt agactacgaa atagatggta tggtaataaa agtaaacaat
    12121 ataaaacaaa gagacgaaat gggatataca gccaaaagtc ctagatgggc aattgcatat
    12181 aaattcccag cagaaaagaa gaaaactaaa atagtagata taatagtaga agttgggaga
    12241 actggtacaa taacaccttc agcaattctt gaaccagtca gacttgcagg tactactgta
    12301 agtagagcta cacttcacaa tgaagattat ataagagaaa aagatataaa aataaatgat
    12361 acagtattag ttcaaaaagc aggggacata atacctcaag ttttagaagt tataaaagag
    12421 gaacgtactg gagatgaaat agattttaaa atgccagaaa agtgtccagt ttgttcagag
    12481 ccaacaataa gattagaagg agaggctgct gtaaaatgta tcaatatgtc ttgcccagcc
    12541 caaataagaa ggggaattat acactttgtg tcaagggatg caatgaatat agatggattg
    12601 ggagaatcta taattacact gctattaaat gaaaaaatta ttcaagatgt atctgattta
    12661 tattatataa aaaaggaaga tgtagtcgac ctagagagaa tgggcgaaaa atctgctgac
    12721 aatttaataa attcaattga aaaatcaaaa gaaaatgatt tatggagact tataaatgga
    12781 ttaggtatta aatttatagg agttaaagca gctaagatac ttgcatataa ctttaaggat
    12841 ttagatgaag taatttctgc aactagtgag caactagaag aattagagga atttggaagt
    12901 ataatggcaa acagcgttgt cgagtttttt aaggaagaca aaaatatgaa cgttataaac
    12961 aagttaaaaa atgtaggagt aaatacaaaa tcattggata atacaggtga aaaagtagaa
    13021 aaaatatttg aagggatgaa aatcgtattg actggtacac ttcctacatt aaaaagaaat
    13081 gatgcgaaag atatgataga aaaaagaggt ggaaaagcta catctagtgt tagtaaatcg
    13141 acaacatttg ttttggcagg tgaagaagct ggttctaaac ttactaaagc aaatgattta
    13201 ggaattaaag ttatagatga agagagattt ttagaattat taaagttatc atcgaaagat
    13261 gaagtaaaag ctgtattaga gagttaaaaa gtcgcttaat ttagtagaaa actactataa
    13321 ttaatgatag aatagttcag taagctaata agattacgaa aaatgacaat aattaatatt
    13381 tgacaaaatt aaaaattata aaaataaatt tataaaaaat aaaaaatatg agaaaggata
    13441 taaaaaatga ccagaagtta tgatagaagt aacgaacaga taagacctgt aaagataact
    13501 agagattata caaagtatgc agaaggttct gttcttatag aaatgggaga gacaaaggtt
    13561 atatgcactg catcagtgga agaaaaagtt cctccttttt taagaaatag tggtactgga
    13621 tggataaatg ctgaatattc tatgctacca agagcaacac aacaaagaaa gataagagat
    13681 gcatctaggg gaaaaattga tggaagaaca caagaaatac agaggttaat aggaagagca
    13741 ataaggtctg ttgtagactt gaataaatta ggagaaagaa ctatttgggt agattgtgat
    13801 gtgattcagg cagatggtgg aactagaact gcatctataa caggggcatt tgtagcagtt
    13861 gcagaagcaa tatataaatt atataaagca aagacaataa aagagatgcc aataacaaat
    13921 tttgtatctg caattagtgt tggtattgta aatggaatac acatgctaga tttatgttat
    13981 gaagaagatt ctaaagcaca ggtagatatg aatataataa tgactgataa gtgtgagttt
    14041 gtagaagtac aggggacagg agaagagaaa cctttttcaa gagcagattt aaatttattg
    14101 ttagagttag gcgaaaaagg gaataaagaa ttaataaggc ttcaaagaga agctttaggt
    14161 gaaatagcag atgagatatt gggtatggat tatggtaatg aagtagtgat tgctactaat
    14221 aatgctcata agctagaaga aataggtgaa atcttaaaag attttgaata taaagtgtac
    14281 tctttaaaag atgttgattt agcaggtata gagatagtag aagatggaaa aacatttgag
    14341 cataatgcgt tgataaaagc tagagcaata gcaaaaaaga ctaaacttat agcaatatca
    14401 gatgattctg gcttagaagt agatgcttta ggcaaaaaac ctggtgttta ttcagctaga
    14461 tatgcaggag aacatgcaac agatgaagaa aatagaaaaa aactgttaaa agctatgcaa
    14521 aatgtaccaa tgagcaaaag aaatgcaaga ttcgtctctg caatagcagt agtttttcct
    14581 gatggaaaag aatttgtagt aagaggaatt tgtgaaggaa tgataggatt tgaagaaaaa
    14641 ggtaaaaatg gttttggata tgatagctta tttatagtaa aaggatatga taagactttt
    14701 ggtgaaatac ctagtgttat aaaaaattca atcagtcata gagcaaacgc actaaaactt
    14761 atgaaacaag agtttataaa aagggtagtg aagtaatgaa aataggcata gtaagcgaca
    14821 cacatatgat gattaaaaat atggaaaaaa caataccata tttgaaagaa tgtgacctaa
    14881 taattcatgc aggagataac tttactgatt caagatatat tcatagcatg actaatgtag
    14941 gtattattgc agttaaaggt aattgtgact ttgatgctgt tgaggaagaa gttgtctttg
    15001 aagtggcaaa taaaaccata ttcttatgcc atggagataa gtatggtgta aaatatggaa
    15061 ctaatatgtt agaaaaaaag gcaactgaag ttgatgctga tatagttata tttggccata
    15121 cccatacccc atttagagaa attaaagatg gtgtacttta cataaatcct ggaagcactt
    15181 cacttccaag aggagtatcg tataaaagtt ttgtcattat ggatatagaa gaagatgaca
    15241 taaaaattga agaaatacgc atataaaggt gtatttatct aaaaaaatgg caaaaaacgc
    15301 tcaaaaaatt aaattttatc aaataagtac aacgatatta atggaacact tgtaaataaa
    15361 aaaacttatg tgatataatt aagtggttga attataaata gaatgaatta taataccgtt
    15421 atcgggtatt acataaatat cactaggagg attagaagga tgaaagcaga actaattaaa
    15481 aaagagggta ataaagttac tttaaaaata acagtagata acaataaatt tgaagcagca
    15541 gtaaataagg cttataataa aagcagaaat aaatacaata tacctggatt tagaaaaggt
    15601 aaagctccaa gaattgttat agagactcaa tatggaaaag gtatattcta caatgatgct
    15661 atagaaatat tattcccaga agtttatcca gaagctatca aggaattaga catagaccca
    15721 atagacaatc cagatttaga tatagaagaa ataagtaaag ataatgggtt ggttatggtt
    15781 cttaatgtag aagttaaacc agagtttgaa ttaggaaact acaagggtat agaaatagct
    15841 aaggttgaga atactgtaag tgacgaaaat gttgatgcta aactacaaga aatggttgaa
    15901 aaaaatgcca gattagtaag tgtagaagac aaagctttag aagatggaga tacagctata
    15961 atagacttcg aaggttttga aaatggtgta gcttttgatg gtggtaaagg tgaaaattat
    16021 aacttagtta taggttcaaa cacatttata ccaggatttg aagaacaatt agtaggtaaa
    16081 aaagctggtg aagaagtaga agttaatgtt actttcccag aagaatatca ctcacaagat
    16141 ttagctggaa aaccagttgt atttaatgta aaaataaatg atgtaaaagt aaaagaatta
    16201 tctgcacttg atgatgaatt tgctaaagat actagtgaat ttgattcttt agatgaatta
    16261 aaagcagatg taagagctaa gttagaagaa gaagctaaaa atagagcaga tgcagaaact
    16321 agaaattcag tagtagaaaa agttgctgaa aatacagaaa tagaaatacc agaagttatg
    16381 atacaacatc aaatagataa tatgttaaat gaacttaact accaattaca atatcaagga
    16441 tttggattac aacaattact agagatgact ggtagaacta tggaagaatt aagagaagag
    16501 agaaaagaag atgctaaaaa attagttaag tcttctttag tattagaagc aataactaaa
    16561 gctgaaggaa ttgaagcaac agaagaagaa tttaaggctg agttagaaaa aatggcttct
    16621 gcttacaata tggaagtaga aaaaatagaa gcttctttaa gagatgctga caaagaagat
    16681 ataaaaggtc aaataaaaat aagaaaaaca atagatttat tagttgataa tgcaacaata
    16741 gcttaataaa ataaacgcta actaatatag ttgacgttat gaaatgttta gtatataatg
    16801 atgtgcaaat ataatttagt taaatgcgta taataattta cagtagctta aaaaagctac
    16861 tgtaaattat atagaataaa gggggtatga aaccatggca ttagtacctg tagttgttga
    16921 acaaacagga agaggagaaa gatcttatga tattttttct agactattaa aagacagaat
    16981 aatcttctta ggagatcaag ttaacgatgc cacagcaggg cttatagtag cacagctatt
    17041 atttttagaa gctgaagacc cagacaaaga tatacattta tatataaatt ctccaggagg
    17101 aagtataact tctggaatgg ctatatatga tactatgcaa tacatcaaac cagatgtaag
    17161 tactatatgt ataggtatgg ctgcttctat gggagcattc ttgttggcag caggagcaaa
    17221 aggaaaaaga ctagcactac caaatagtga aataatgata catcaaccat taggtggagc
    17281 tcaaggtcaa gcaacagata tagaaattca tgcaaagaga attttaaaaa taaaagaaac
    17341 tttaaatgag atattatctg aaagaactgg tcagccatta gaaaaaatta aaatggatac
    17401 tgaacgtgat aacttcatga gtgcactaga agccaaagaa tatggtttaa tagatgaagt
    17461 atttacaaag agaccgtaga tagagagagg tgttaatatg tctaaatatg aagaaaaacg
    17521 acaattaaag tgttcattct gcggtaaaaa tcaagatcaa gtaagaagat taattgcagg
    17581 tccaaatgta tatatttgtg acgagtgtgt tgaactttgt gacgaaatta ttcaggaaga
    17641 aatagaagat actatagatg aagatactac atctttacca aaaccaaagg aaatgatgga
    17701 gattctaaat gattatgtaa taggtcaaga aaaagctaaa aaagctttat cagttgcggt
    17761 ttataatcat tataagagaa tatacagtaa aaaatctagt agtaaggata tagaaattca
    17821 aaagagtaat atacttttat taggacctac aggttctgga aagacattac ttgctcaaac
    17881 tctagcaaga actctaaatg taccttttgc aatggcagat gctacatcat tgactgaagc
    17941 tggatatgtt ggagaagacg ttgaaaatat acttttaaaa cttattcaag ctgctgattt
    18001 tgatatagaa aaagctgaaa gaggtataat atatatagat gagatagata agattgcaag
    18061 aaaatctgaa aacccatcta taactagaga tgttagtggt gaaggtgttc aacaagcact
    18121 tctaaaaata ttagaaggta ctgttgcaaa tgttccacca caaggaggaa gaaaacatcc
    18181 ccatcaagaa tttttaaaga tagatactac taatgtatta tttatattag gtggagcttt
    18241 tgatggatta gagaagataa ttcaaaaacg tggtggagat aaaacactag gttttggagc
    18301 taaaatagaa agtaaaaaag aattagattt agggaagctg tatgaaaaag tacttccaga
    18361 ggatttatta aaatatggta taataccaga gtttatagga agaataccag ttttagctac
    18421 tttagagttg ttggatgaag atgcattaat gcagatatta caggaaccta aaaatgctct
    18481 agtaaaacag tataaaaagt tattggagtt agatgatgtt gagctagaat ttgaagaagg
    18541 tgctcttcgt gcaatcgcta aaaaggctat agaaagaaat acaggagcta gaggacttag
    18601 aagtatagtt gaaagcgtaa tgatggaaac tatgtttgaa gtaccatcta gagataatat
    18661 aaagaaggtt atagtaacag agaaatctgt gaacgaagac tcagtaaatc ctatcatagt
    18721 acttaaggat caagaggaaa gtgcataaga tgatgaaaag gagctttaaa agctcctttt
    18781 tttatgttaa tatgctaaat ataaaatagg tatcattatc ataaaaatgt gagtaatata
    18841 cctagaatac caaagacgaa tacaattgca atggaaccaa tttttccttt atataaaaga
    18901 taaaatgatg ctataaatgc aattattcca tataaatccc aattaacttc tttggcagat
    18961 gaaaaagctg ctgataatat gaatcctaga gtaattggtc taagaactgt caataagttt
    19021 tctacctttt tattattttt aaatttagaa aataactttg atataatagt tagacctatt
    19081 aatgcaaaaa ttgtaacacc aatagttgca gctagagaac caaagaagcc agcagtctta
    19141 aatcctacaa atgtagcact atttattgct acaggacctg gtgtagattg tgatatagca
    19201 agtaaatcca aaaactcttg attagttata aagtgatatt tagatataaa ttcttgctgg
    19261 ataaatggta gcatggcata accgccacca aaactaaatg cccctatttt aaggaatatt
    19321 aagaagagta gtattatttc tgacatagac tatatacacc tcctccaatt attaaataaa
    19381 tagggcttat attgaataca ccaacagata aaactgctat aattaatagt agtatgttag
    19441 ttttagactt tggtagtttc ttgaacatgc tcgtaaaaga atacaatata agagctatta
    19501 caacaggcgc aactccttta aaaaatccat ctaaaatttt agatgatgaa tttttaaagt
    19561 atagatacga taagactaag actatacaaa atgatggtaa agcagagcct aaggtacata
    19621 taagaactcc tggtatacca taaagtttat atcctaataa tacagatata ttaactgcca
    19681 gtactccagg ataactttga gctatggata aataatcaat aaattcttcc tgctctaaaa
    19741 gtttttgttt gtttacaagt tcatcttgta taagaggaac catagcgtat ccaccaccaa
    19801 atgtaaacgc accaattttt aaaaaggtta aaaacaattt caacataata gaaacctcca
    19861 aaagataaat aacatctaag tatactaata ttaatattat atcataataa aaagataata
    19921 tagtatgata gtattctaag aatttatcat taatgttgaa aattaacttg ttcaaatata
    19981 taatattaaa agcaaagcaa ttagactaga aaaggagtaa tgttgatgga acaaaattat
    20041 actaaaatag atcatgaatt accattaatt cctctaagag gattagctat atttccatat
    20101 atgatattaa attttgatat aggtagagag atatctctta aagctttaga ccaagctatg
    20161 atggatgaag aacttatatt tttaacttct cagaaagaag cagaggtaga tgagccagga
    20221 gaagaggatt tttatcatgt gggaacaatc tgtaaagtta agcaaatgat aaaattacca
    20281 ggagatacag ttcgtgtttt ggttgaaggt gtatcaaggg gtagagtaaa gaaaatagaa
    20341 caagaagatg ggtatttcag agcagtaata gaagaaatag tatttaatag tgataatttg
    20401 gatagtgaaa cagaagttga aattgaagca tttgtaagaa atgtatttga tgcctttgaa
    20461 gagtatataa atattggaaa tagggtatca ccagaaatat taatatctct agctgatata
    20521 gaagatgtag atagatttat agatacgata gctgcaaaca tatatctaaa atcaagccaa
    20581 aaacaagaaa tattagaaga atttgatatt agaaaaagac ttgaacttat ttattctata
    20641 ttattagagg aaatagatat attaaaaata gaaaagaaaa taactttaag agtaaaaaaa
    20701 cagatgaata aggttcaaaa agaatattat cttagagaac aattaaaagc tatacagaaa
    20761 gaattaggtg aagaagaaga tataaattct gaagcagatg agtatagaga aaagcttaaa
    20821 aaaataaaag cacctaagac aaccaaagaa aaaatagaaa aagagataga taaattttca
    20881 aaaatatctt ctatgtctcc agatgtatct gtaagtagaa actatttaga tactatattc
    20941 tcattgccat ggaataaaga gacaaaagat aaattagata taactaaggc aaaagatata
    21001 ttagatgaag accattatgg attagaaaaa gttaaggaga gaatactaga gtatctagct
    21061 ataagaactc ttgctaagtc acttaaaggg cctataatat gtctagttgg tcctccagga
    21121 acaggaaaga catctatagt aaaatcaatc gccagagcat taaatagaaa atttgtaaga
    21181 atatctttgg gtggagttag agatgaagca gaaataagag gtcatagaag aacttatgta
    21241 ggttcaattc caggaagaat aattaatggg gtaaaagaag cccaaacaaa aaatcctgta
    21301 ttcttatttg atgagataga taagatggca gcagattata aaggtgaccc agcttcagca
    21361 atgttagaag tattagaccc agagcaaaat aaggactttg tggaccatta cttagaaata
    21421 ccttttgatt tatcaaaaat actatttgtt acaacagcaa atagtctagg taatatacca
    21481 agaccattat tagatagaat ggaagtaata gaagtatctg ggtatataga agaagaaaaa
    21541 ttaaatatag caagaaaata cctacttcca aaacagatta aagaacatgc tttaaaagaa
    21601 aactttataa aaatagatga tgaaacttta agaagtataa ttaaccatta tacaagagaa
    21661 gctggtgtaa gaacactaga aagaactatt ggtaagattt gtagaaaggt tgccaaaaaa
    21721 tacgttgaag acccaactct tgaagaggtt gttataaata agtctgacct agagacatat
    21781 cttggaaaag acatgtttaa gtatcaactt gcagaagtta atcctcaaat aggattagta
    21841 aatggtcttg catggacagc agttggtgga gttacacttg aagtagaagt aaacgtactt
    21901 aaaggtaaag gagaaattgt attaactgga aaattaggag atgttatgaa ggaatctgcg
    21961 aagacaggta tctcttatat aaggtcaata gtggacaagt ttgatataga ccctgaattt
    22021 tataagacga atgatattca tatacacatt ccagaaggcg ctgttccaaa agatggacca
    22081 tcagctggta taactatggc tcttgcagta atctcagcac ttactaaaag acctgttcct
    22141 ggaaatatag caatgacagg agagataact ttaagaggta gagtcttagc agtaggaggg
    22201 gttaaagaaa agttattagc agctcataga gctggtataa caaaagtatt aattcctaaa
    22261 gaatgtgaag ctgatttaga tgaaatacca gaaaatgtaa aagaaaaaat ggagtttgtg
    22321 cttgtagaac atatggatga agtattagaa caagctttat taaagagtgg tgaaaataat
    22381 gaaaattaga agttcagaga taactatgag tgctgtgaat aaatcacagt atccagcaga
    22441 aggaatccca gagatagcat tggctggtag gtcaaatgtt ggaaaatcat caataataaa
    22501 tacattgctt aacagaagaa acttcgctag aaccagtcaa acaccaggaa aaactagaac
    22561 tataaatttt tatctaataa ataatgaatt ttattttgta gaccttccag gctatggata
    22621 tgcaaagata gctaagtctg aaaaagaaaa gtggggaggt ataatggaaa gatatctaga
    22681 aagtagacaa gagttatgtt ctatattcct acttgtagat attagacatg agcctacagc
    22741 agatgataag cttatgtatg aatggataaa gcactttgga tataactgtg tagttattgc
    22801 aactaaggca gataaaatat ctagaggaca atatcaaaaa cacataagta taataagaaa
    22861 aaaattgcaa atggaaagta gtgaaaaagt aattcctgtt tcttcactaa aaaagactgg
    22921 tgttgaagaa ttatgggaag agattgtaaa tcaatacaat caacatggat atgaaataac
    22981 agtagattaa atttaaaatg ttaaatattg acataagatt tatttgaata ataaatgtaa
    23041 taatagttag ttttaattat attaatatta aagcaaaata taggatgcct taaaatagtt
    23101 ttaatgaact ttttttggca tccttattgt ttataaacaa taaatatcag taattttgct
    23161 aaatactaat aaattttagt aaggtatggg aaaattagta taaattagtt caactaaaaa
    23221 attattgact tatgtagtca gcaataaata gcatttaaga aatagattta tctgtttttc
    23281 tcataaagaa aacagttagt ataagcccta taaaacatac tgatgctgca atccaataag
    23341 actgattgct agctataaca taggctgatg tgtgactttt aaacttttga ttgtatgtaa
    23401 gactttgtgc aaaaaaacta ctaaaaaatg ctgttccaag agcaaatgat aaattacgaa
    23461 tagttgcaag aaaactgcct ccgtacccct gtttttgagg aggaacatct gctaaaatac
    23521 tactattgtt tggtgaacta aatgctccta gcccaagtcc aaaaataact aaacatacaa
    23581 ttaaaagata caatggagaa tctgctttta aaagtgccat tccaactagt gagatggtca
    23641 taaatgaaaa agatgctgtt agaatgtttt ttgcaccgtg tttatcagaa agtgaacctg
    23701 caataggtgc tataagtacc ataataagtg gatgtacagt cattacataa ccagccatca
    23761 taggagataa attttttaat tgttctaaat aaaatggaag tagaaaagat actgccattt
    23821 gaggaaaata acaagctact ccaatgatat tgcctacagt aaagtttcta tttttaaata
    23881 gaggtaggtc aataaagctt tgttcaaatt ttacttctct taatgccaac aataagaaga
    23941 ttataagtgc tgttatacct aaatacaatc ttgtattacc cttctcattc attgcaagta
    24001 atataagcgt agtgcaagat gcgaaaagta gaagacctga aatatcaaag ctcttgtttt
    24061 gttcatccaa aacaggttta ggcagaaatt taatgccaag aaagactgct ataataccaa
    24121 taggtatatt gataataaaa atagaaggcc atccaaaatg ttctaaaatt atacctccaa
    24181 ttacaggacc actcatattt cctataccaa ctacagctcc agtgattcct atagcttttc
    24241 ctttttcatt tgctggaaat gcattggaaa ctataccaag cccagtagcc attaaaatac
    24301 ttgctccaac tgcctgaaag attcttgaag aaattaatgt tgataaattg tttgacatac
    24361 tacacagaaa agaacctatt gtaaatataa aaaatcctaa tgcgtataga cgatttgaac
    24421 ctactttatc accaagtttt ccgaaaaata acatagtagc tgttacaaca agcatataag
    24481 ctgttacaac ccactgaatt tgtgacatgt taattttaaa gtcagcagca attgtaggat
    24541 ttgcgatatt tagtatactt ccatcaagag tagacataaa tacaccaata cagatgataa
    24601 tcaatactat ccatttttgt ttcatgataa cactccaatc ttaatagatt ttataagtac
    24661 gatataattg taaaataaac aacaaaaaaa gcacctgtat attaggtacc ttgaatgtgt
    24721 ctaatttcat gaataaatac ggtagttgaa ggatttgtat agattactca tgtaaaatta
    24781 cataaaggta ctcatatact attttcttct accatccaga ttttactgtc ggctttggaa
    24841 tatgaccaaa ttatgccata aggctcgcgg gcttttaccg ccgataggga atttcacccg
    24901 tttatgaaga aaaacagatg tattttgtaa tattatttta acataaaatt ataatggaaa
    24961 caattggatt taagtaatat ccagctgtat tacaatacaa atatttcaat atggacactt
    25021 aaattgtatt tatagataga ataaggtaca gtatgagaat ctataataat tagataaaat
    25081 taatatgtta ttagtattag atggtaagtt ttaaatttgt caaaatcaga aagctgtaat
    25141 aaaattctta taatatgaat atcttaatta agagatattg taaattatga ggaggcgcta
    25201 atatggaagg aaaggtaaag aaggcattcc caggtggaaa tacatcacaa ggctcatatt
    25261 ctttttttga ctatataatt cctgaaaatg taaaaagagt tttttgttta aagggtggac
    25321 ctggagtagg aaaatcctca cttatgaaaa aaatttataa agagtttttg catagaggat
    25381 atgatattga tttatatcat tgtccttctg acccaagttc gttggatggt ttagttataa
    25441 agaaattagg cgtggtactt atggatgcaa ctgccccaca tactatggac ccaaagctac
    25501 ctggagcttt ggatgagata attaatttag gtgattactg gaatacagaa gaattagaaa
    25561 agaataaaca agaaatatca gtttatgata aagatattag taattcattt aaaagagcat
    25621 ttaagttttt aaagtcagca gaaccaattt ttagagacat agaagaaaaa tattctgatt
    25681 gtatggatta tggcaaagta aatttggtca cagaagaatt tataaaaagg ttatttgatg
    25741 gagttaaaag cacaggaaat ctaaaaagag aaaagcattt atttggttca gctataactc
    25801 ctgtagggtg tttagattat acagaaaaca ttttaaaaga tgtaaagaaa gtctattatt
    25861 tagatggaga aataggaact gggaaaacta cattattaaa caaagtatat aagaaagcta
    25921 ctgaaaaagg tttaaatgta gaagtttatc attatccact tattccagag aaaattgaaa
    25981 ctgtactatt aactgattta gatataggca taactataag tacgactttt aaagaagaag
    26041 atacaattga tttaaatcag tttttaaata gagaaaaact acttaaatat gaagaagata
    26101 ttaaatttga tgaaaaagtt ttagatgatt taatttgttg ggcagtacta aatcttaaga
    26161 gagccaagtc taaacatgat attatagaaa gttactatag tcctaatatg agatttgatg
    26221 aagtagggaa attaagagat aagttaatta aaagaatttt aaaatatgag gaaaacttat
    26281 aaacgtatat gtttaaaatt aatgttaata ataatataat ttttagtaaa ttggtgtagc
    26341 tttgaggcta tgccatttta cttttttttg ctaaaaaatg atattaataa tctccaaagc
    26401 cattatttgt ttttcataat tattatttcc tttcctaatt tatgtttttt tataatatca
    26461 caacaatgtt tattgaaagt ttatataatt ttaagaacat ctaaaaatgc atacttgata
    26521 ttggtaaatt agtgatagga gttcaatgta tgatattata tactaaagat gaattttaat
    26581 atataacttt gatataataa gcatatgtaa atgtttaata tttaattaaa gaaaattatc
    26641 atataaaaat aaatttgtaa ggagcaatac agtatgaaaa aacaatatgt gtttataata
    26701 ctagcaagta ctattttagg tatacttgtt ggaaccatat ttagagatta tagcaatgaa
    26761 gaaacagttt atacaagcaa agacaagtca ataagaaaag aaataaaaat gacaagaaaa
    26821 tctataaaag aattaaccaa agagaaagac aagttagata aagaaattaa ttcaatgaaa
    26881 caaaatcaaa aaaattctga agaaatatca gaaatagaaa cacttagaga tagtctatca
    26941 tattcagatg taaagggtag tgggataata ttaagtatag atgctataga tgaaaaaaca
    27001 gggaatatag ctaatagtat agactataat aagattcttg taaaagttat aaatgaatta
    27061 aaagtaaacg gaggaaaata catagaaata aatggtcaaa gaattaatca atattcagaa
    27121 gttgtacttg caggaagcca tataaatata aatggagttc ctgtagcaca accatatgaa
    27181 ataaagtgca ttggagataa ggagaaatta tctaattatg tagataaagg tagtaaatat
    27241 ttagatagca tacaaaccaa ttatccaatg aaaataagca taaaaactga agaaaatatc
    27301 tctatgaaaa aaatgaatat acctaataaa ttaaagtata taaagggtga ttaatattat
    27361 attagaggaa agcagtttgc caatgttaat accagaaaaa cttgcaaaga aatataatgt
    27421 atttccagtt gagtttcaag atgataattt agttgtagaa atagaagatg aggatattta
    27481 tgctctacag gacttgagat tagctacagg taaagaaatt atattgaaga aggagaaaaa
    27541 agaacttata agtgaaaaga ttgaaaaata ttataatagc tttgaacttg atgaggatta
    27601 tgcaaaaaaa ttatttgaaa atcttttaga aaaagctata aaagaaaatg caagtgatat
    27661 acatatagaa ccatttaaag aacatttaat agtgagaatg agggtagatg gagaactaaa
    27721 ggaagtatca aagtttttag ttgatgtcta tccatcattg tctacggtta taaaattaaa
    27781 ggcatcaatg gatataacag aaaaaaggct tcctcaggat ggtagggtgg atataaagtt
    27841 aggtggaaat ttaattgaca ttagagtttc atcaatacca actgtctatg gtgaaaaaat
    27901 agttttgagg atacttaata gagatacttt tttaaaagac aaattagaac ttggtttttc
    27961 agaggaagct ataaaatcta taaatcgtat aataaataaa agagcaggag tattattagt
    28021 gacaggtcct acaggtagtg gaaagactac aacagtatat tccttattaa atgatttaaa
    28081 aggaataaat aaaaatatta tgactataga aaatcctgta gagtataaga tggatggcat
    28141 aagtcagatt caagtaaatt caaaagtagg gttgagcttt gatgtaggtc ttagagcgat
    28201 attaagacaa gacccagata taataatggt cggagaaata agagatgcag aaacagctaa
    28261 gatagcagta agagcagcta taacaggtca tttagttata agtactttac atactaatga
    28321 tgcagtatct tccatagcta gattactaga aatgcaaata ccaccatatc ttttaaatgc
    28381 ctctcttata ggtgttatat ctcaaaagtt ggttagaaaa gtttgtaatc attgtagcca
    28441 tgagattatg ataaaagata attttagtgg agatgtaaat actaaggtag ctgtagggtg
    28501 tgaaaaatgt aatgataaag gatactttgg aagaacagca atttatgaaa tcttagaaat
    28561 taatgatgat ataaagacat gtataagaaa tatggaggat tctagtacta ttaaagatgt
    28621 ggcacaaaat aatggaatga taacatttga agatagctgt aaaagactaa taaatgaaaa
    28681 aataacgaca ttagaagagt gtttagtagt aaatgaaatg atttaactga attgtatagt
    28741 taaacttcgt ttggtcagtt gtctttattt aatctatttt aattaagatg tttgagtata
    28801 aattatattt aagtgaaata aaattaaatg cattaaatta gaaaatatta agtttattta
    28861 attgtaatta atataatttg gaaggtatta tagtacatga gattatctaa aaatatattt
    28921 tataatttag aaaagtttta tttagtatta aatagaatca gtgataaaga aattagagta
    28981 atttgcaaag agataggcat attgctggaa tctggatgtg aaattacaaa aatatttgaa
    29041 atcattgaga gtcagtcaag taaaaaagta aagaatttgc ttagtatagt atcaaatcat
    29101 attcaaaaag gtaactcaat agcagaatca tttcaaataa caggtatatt ttctaaattc
    29161 tttataagta tgataaaagc aggagaaact agtggaaact tagatataat aatgagtgat
    29221 ttatctaatt attatgataa agaatacaaa ttaaaaatga aaataataac tatttcaata
    29281 tatccaataa tacttataat attatctatc ttatcaatgt tgtttatatt cgtatttgta
    29341 atacctaatt ttcaggtggt ttttaccaac aatggaattg aaccaccttt aataactaga
    29401 gttttaatgg gaatatctac agtggttaca aataacttag cttatataat atttagtttt
    29461 atattgtttt caataggaag catttatttc tttataacaa atgataatat aaaaaaattg
    29521 attaattcgt taaaattcaa aatacctttt ataaaaaaaa ttaatcagtt agtagctaca
    29581 acaagatttt caagggcatt gtccgtttta attagcagtg gaatacaaat tgtggaagct
    29641 atagacatgt cagctagtgt tatagacaac gaatttattt atgaaaggtt aaagatttct
    29701 aataattata taaaaaaagg aaatagcata ggtaaatctt tgaatttagc aaatgttttt
    29761 ccaaaattat ttatttcaat ggcaaataca ggagaagaaa gtgggaaact agataaaagt
    29821 ctaaatgtaa taaataaatt ttatgaaaat gaattaaata acaaaataga gcaaataatg
    29881 aaatttatag aacctatgct aattgtattt atgggattaa ttattggcac ttttatttta
    29941 gcaatggttg ttcctatgtt tgatgcaatt actaattttt agagagaaaa ataaatttta
    30001 ttagatgaaa cttttgaaaa ggagagaata tttaattgat aaatttgata aataaaaaac
    30061 gaaaaggatt tacacttgtt gaaatgattg tagtagtaac tattttaggc gttatatcta
    30121 gtatagcatt agttaagtat agtaaggttc aagaaagtgc caaattaaat gcagactata
    30181 cgaatgctgc taatatagta actgcagcta gcatggcaat taatgatgat gaaaagacaa
    30241 tagactctct aagtgtagaa acattgaagg aaaagggata cctaaatact gttccagttc
    30301 ctcagagtac atcaggtaaa ttcgaacttg tcataaatga tagcggaaca gatataagcg
    30361 taaatataaa ttctaaacaa ttttatccaa aataatattt tatattgatt gctgtaagtt
    30421 ttaaaattat ttttatttaa gaaggagatg gctataatta aaaaaattct aaacttagaa
    30481 ataaatgatg attatataag gcttgtattt ttaagaaaaa gtcataaaaa agtttttata
    30541 gataaatcaa ttataaaaga gattaacttt gatataaaaa atgatagtaa ttcgattgag
    30601 aagattaaat atatagaaga tgtagttaga tttgtaaaag aggagattgg taaatcaaaa
    30661 atattatata aaaatttata ctttaacatt caaattcaag atatagtcat tagaaatgtt
    30721 gaagttctaa atactaagaa aaaaagagac atattaagca tgattgaatt tgaaataaca
    30781 caatacattc caataaacat aaataactat tcaataaaat ataaagttat aaattctaaa
    30841 aatgataaat tgaatataca agtaattctt ttcccaaaga gtattattca attaattaag
    30901 aacatttcag aaaatctaaa tatgaatcca aagactataa atataaattt tgatatatta
    30961 caaaagctta taaacttaaa tttaatagag aatttttcag aagaaggggt atttatagaa
    31021 tgtaaaaaaa gtcagttaat tataaatata acaaaagata aaaaaataag agaaacatac
    31081 ttgctatcaa aagagggaaa atcatatgag ttcataaaaa gattattaag ggattttaag
    31141 tatgtttatt actatggtat agaaaattgt tttattgaag aatactttaa atatacagat
    31201 aaatctattg aagtaaatcc attaagatta aaagataaag taaaagtaga aaataatttt
    31261 gatgcagatt tagaagacaa taggataaat tatataaata gtataggaat gattatttaa
    31321 gtgaaagtgg ataaaacaat aaattttctt aatggattta aaaaaactaa accatgtaat
    31381 aaaatctata aaataataat agtcttaatt ttaacgtgta ttttatttaa atcatttatg
    31441 tatgtgaaat ctataaatga acttgaagat tatataaatc aaaataagct agtggataaa
    31501 tcaaagcaca ctttaccaac aacagataaa actagagaga aattttctaa aataaaactt
    31561 aatgaaataa aaaaaatata tgaaacaatt ggaataaata gtattactaa aatatcgata
    31621 gataaaaata agctagagat ggaaggtgtg tgtagtgatt tagatgtttt aaacaaccta
    31681 aggaaaataa agtacataaa atattattac atagacagta ttgtaagaga tggagaatat
    31741 tatagattta gaataaaaag ttagataggt gattattttt gagattaaat aatagagaaa
    31801 aaaatctact taaaatattg ttaatattag ttattatatt ggcaagtgat atagcagtaa
    31861 gttatttgga agaaaaccta actagtttga agaatgaaaa gcaaaatata ataaataaaa
    31921 taaataatca agataattat caaataaagg ataataagca tatagatgat aaatatatag
    31981 aaagtgttga attaaataaa aatgaagaat tgaaaaataa ttttaataac tcatttttag
    32041 ataaaaaaac aaataaagat atagtgttat tagtagaaaa tgaactgaga gatattgtga
    32101 atgtagaata tattaatcaa gaaagtagtt tagataatca caataaaaaa caagttaatt
    32161 tagaaataaa agttaatgga gaacttgcaa atgtgttgaa acttgaaggt gtaatgaaga
    32221 agttaagact aaataataaa cttgaaaaga tagaaattaa caaattaaaa gtcaataatt
    32281 ttgatgttca aaatattcca aatgaatata aacaagaaaa agacaatatt gttgaatgta
    32341 aaatgacagt tagagtcata taacaaaaaa gaatagataa agtaaaaaac tattatcaat
    32401 taataaaatt ttatagagga attagtttta cgataattca tttctacaag agggattgtt
    32461 gatgatttta tcgaaatcct aattaaagtt aatatattgt accaaaatat aagtgaattt
    32521 aatagaatat ttacttatac tatagattga ttaataaaaa aatggtataa taacaaattg
    32581 tactttaatt taataataag ggagtggaag tcatattgtt agggagaaat gagctatgcc
    32641 cttgtggaag tggcaaaaaa tacaagaaat gttgtttaaa taaagatatt gtttcagaaa
    32701 gagcatgcag aaaaactgaa ctttcacaaa aacaatatac agatttatat tcaaagttat
    32761 atgattattc aagacaggat aagtttaaag aagaatgtga aaaagctaaa gaaatgtttt
    32821 atataatgca aaatgatagt gtaaatgaaa aatttgaaag attttttaat acttatttaa
    32881 tacaagacca cataatggaa aataaaaagg ttatgacagt tggttttctt gaagaaaatg
    32941 gtgcaaacct aagtcaatca gaagtatcaa tattaaaaag tatgtttgaa tcatacgtaa
    33001 gtatttatga ggtaaaagaa gtttctgctg gaaaaatagt attaaaggac tgtttaagtg
    33061 gaaatgaact ttgtacagaa gatgtaaaac ttctgagaag ttttaaaatc ggaagttgta
    33121 tgatagctag aatagttgat attgaaggaa ctaacatatt gatagatata actataagta
    33181 tatcaaatga ggttaaagat ataattttaa aggatattat gaatctattc aatcaatatc
    33241 aagatgtata caaagatatg aagtcgttct taatttatca tacacatata ctatacaagt
    33301 atatacaaca gcttttagac ccaagtatag ctgaatattt aaagagagaa agagataaaa
    33361 acgatacaaa agttaattca gaaaatgaat tagctgaaga agaatgtaaa gttttagata
    33421 ctcttaaaga aaatgtagaa aaagaaaata tagatgcatg tatagagttt tggaatgaat
    33481 ataagaaaaa tcatgaaaat ataaaaggag ctgaaaatgg ttgggcagct gctgttgaat
    33541 actatgttaa aaaagaagca ggacaaccta taactcaagt tcaaatatca aagaaatacg
    33601 atataagctc tagtacacta ggaaagagat acaaagattt aaaaatatct taattaatta
    33661 taaaaagctt taagtcatag ataaagaatc tatagtataa tatatgtata atgtttagaa
    33721 agaggtatta ataatgagtc ttagtttagg aaatgcaatt gctgtatcaa cgaactttat
    33781 aaaatctaac ccagctgtac ctgtgtacaa cggagaagaa atatatgcag ttatttatca
    33841 tatagaaata ggatataata atggtgtatc acctgatacc aaacaatata taataataga
    33901 ctataaagag tttgatttat ctatggataa aaaaaatgaa ctaataaatg ttgctaaaga
    33961 gtactgtaaa tctaaaggta ttgtagaaaa cgaaaacctt gaaataaacc ttttagacaa
    34021 tgaagaagca gagaacttct tagaaaaggt gtttaataac atgaaggtta taagaggtct
    34081 tataactaaa aaaggataaa taaatggact atataggaat agaaaatata acaccttatg
    34141 aaaatacata tgaatttagt gtatatgaat atgatgatga aatcacctta ggtagtgaaa
    34201 agttatatgt gtgtgaatta agggttgtat tgattaaagt taattctctg tatgttgaaa
    34261 gattgcataa atcagttgaa gcaatggtct tagtaaaaaa cttgaaaaag gatttagata
    34321 aaacacttgt tgtaaacaaa ataaagaatt ttgtgctaga tgagatttgg gtagaaaatc
    34381 tagtaaaaga gaatatagaa gttatatttg tagaaagcta ggaataaccc tagctttttt
    34441 atattacaat ctaagagagg aaaacaatat gttaagagta tctaatgtaa aacttggaat
    34501 tgataaagat atatctttat taaaagatgt tgtacttaag aagctaaaaa taaaagaaaa
    34561 aaacttagtc aaatatagta tatataaaga atctatagat gctagaaaaa aaggaaaaat
    34621 ggaatttgtt tattctgtag atgtagaagt caaagatgaa aataaaatac ttaactcaaa
    34681 aataaaagat gtaacaaaaa taaaggaaat cagatacata aatgtacctg tgggtgataa
    34741 aaaattaaag aataatcctt tggtaattgg tagtggacca gctggtttat ttgcaggtct
    34801 gcttttagct caaatgggtt atgaaccaat catacttgaa agaggtcttg atgtagacag
    34861 tagaaccaaa gatataagtg atttttggac aactagaaaa ttaaaaaata attcaaacgt
    34921 tcaatttgga gaaggtggag caggaacttt ttcggatggt aaacttacaa ctagaataaa
    34981 agatatacga tgtagaaaag tattagaaga gttagttcag tttggagcac cagatgaaat
    35041 attatactca cataagccac atgttggaac agatatatta aaaaatgtag ttaaaaatat
    35101 aagaaatgaa ataataagtt taggtggtca agttagattt aattcaaaag taacagatat
    35161 agttgtatca aatgattcta tacaatctgt aataataaat gatactgaaa aaatagatac
    35221 agatacaatt atactagctg ttggacatag tgcaagagat acttatgaaa tgttgtattc
    35281 aagaggtata aaaatagttc aaaaaccttt tgcaatagga gctagagttg aacatccaca
    35341 agatttaata aataaagcac agtacaagga tttttataat catcctaggt taggagctgc
    35401 agattataga cttatagagc atgtttcaaa tggaagaaca gcatatactt tttgtatgtg
    35461 tccaggaggt agtgtcatag catctgcatc aaatgatttt gaaatagtta caaatggtat
    35521 gagtgaacat gcaagagata aaataaatgc aaatagtgca tttttagtaa atgtaacgcc
    35581 agaagatttt aagagtgata atccactagc aggtgttcat ttccagcaga agtatgaaaa
    35641 attagctttt gaattaggtg gaaaaaatta caatgcacca gttcagcttg ttggagattt
    35701 tataaacaat aagacttctt tgaatttagg aagtgttgag ccatcatata gaccaggata
    35761 tacacttaca aatttaagtg aatgtttgcc tagcttcgta acagagacta tgagagaagc
    35821 gtttatatct cttgataaaa aattgaatgg gtttgcaatg tatgatgctg ttttgacggg
    35881 agtagaaact aggtcatcat caccaataag aatagtaaga gatgaaaata ctttagaatc
    35941 agtgagtatt aagaatctat atccatgtgg tgaaggcgct ggatatgcag gtggtatagt
    36001 tacagcagca gttgatggta taaaatgtgc agaaaaaata atacaaaaat acaccaataa
    36061 tacaaaaatt taacataaac ttaatgattt tttaatattt aacatgaatt taacattatc
    36121 actcctataa tttgataaaa tacaataggt tataaaattt ttagatacag taatctcttt
    36181 acaaggagga aataatttga acattataat aagtctaatg ggtggattag gtctgttctt
    36241 atatggtatg aaccttatgg gagaaggtct tcaaaaatct gctgggacaa agctaaaaaa
    36301 gataataaaa ctacttacta gtaacctatt tatgggagtt ttggtaggta caggagttac
    36361 agcagtaata caaagttcaa gtgctactac tgtaatggtt gttggtttcg taaatgcagg
    36421 aataatgact ttaaaacagg caataggagt tataatgggt gctaatatag ggactactgt
    36481 tacagcccaa ttggtatctt tcgacttaac tggtatggcc ccagtagcat taggtgtagg
    36541 tattatactt tatttatttg gaaataagcc tagaataaaa aatatagccg aaatacttat
    36601 aggttttggt atattattta caggtatgga ttttatgaag atggcagtgg agccactaag
    36661 agactatcaa ggatttactg acctacttgt tacatttggt agatatccac tacttggatt
    36721 attattggga tttgggatta cagcaataat acaaagttca agtgcatcta tgggtatgtt
    36781 ggttgcactg gcagcagaag gtctagtgcc attatcagca gcactgccaa ttctttatgg
    36841 ccaaaatatt ggtacatgtg taacttcact gttatcaagt attggtgcta acaaaaatgc
    36901 acgaagagca gctatgatgc atctaatctt caatgtttta ggaacggtga tattcttaat
    36961 attcttgaat aaaccagtag taagcatggt tacaagttgg gatccaagca atgttgcaag
    37021 acaaatagcc aatacacata cattgtttaa tataatatct gtattgatac ttttaccatt
    37081 tacaaatctt attataaaac tagctataaa gcttgttcct gatagagcag gtgatataga
    37141 tgaagatgaa actaaaacta ttaaatatat agatgataga atgatagaaa ctccttctat
    37201 agctcttgca aatacgataa aagaagcact tcgtatgggt gagaaggcga aggaaagcct
    37261 aaatgcatct atggaagcct tagtagaaca ttcaactgaa aaaatagcta agacatatag
    37321 acgagagaga ttaatcaatg atttacagaa agctatttta aattacttac ttaaactttc
    37381 aaaagcacct ttagatgatg attcaagaga agttgtggat actttgttta atacagtcaa
    37441 tgatatagaa agaattggtg accacgctaa aaatatagct gaattatcgc aagttgcaat
    37501 agattcaaat atttctttct cagaagaagg acaatctgaa cttgatgtta tgtataatag
    37561 agttgtttca gcatatactt atgcattaga atcaatgaga actgataatg ttgatttagc
    37621 ttgtaaggtt ataaaaattg aagaacaagt tgatataatg gagaaatctt gtagagctaa
    37681 tcacatgtat agattgaaca ataatctatg tagtatagaa aatggagtta tttatttaga
    37741 cgtaataagt aatttagaga gaatatctga ccatgctgtc aatatagcac aacaagttat
    37801 agcgaagaga ttaggaaatg attaaaaata attaatttaa aataatataa aatttaaaat
    37861 agaggataaa taaaagagaa actttaaatg atacgtttag agtttctttt tttgtaaatt
    37921 tatttgacta aaatttttaa atttatacat agttatagta tttagaagaa tcgatttcct
    37981 gataataaat gatgagatat gtatgatttt gtggcaaact tttaaaaaaa acacctatat
    38041 tttgtgttat aatctcttta aaacgaatat gctgaaaaga gggatttaaa tgggaaaaat
    38101 aaattttgat tatagtaaag ctacagagtt tttttgtcaa aacgaaatag atgtcatgca
    38161 accatatgta gatgttgcgc acgacatgtt acataataaa actggtttag gaaatacttt
    38221 tttaggatgg atagaccttc ctaaaaatta tgataaagaa gagtttgaca gaataaaaaa
    38281 atctgctgag aagataaaat cagactcaga tgttcttctg gtaataggta taggaggctc
    38341 ttatttaggt tcgagagctg ctatagatat ggtaagtcat tcttttagga atggacttaa
    38401 aaaagagcaa agaaaggctc cagaagtata ttttgtaggg cataatataa gctcaacata
    38461 tattatggat ttattagata taattgaagg aaaggatatt tctgtaaatg taatatctaa
    38521 atcaggaact actacagaac cagctctggc ttttagaata tttaaagatt acttagagaa
    38581 aaagtatggt aaggaagaag caagaaagag aatttatgca acaacagatg caagtaaagg
    38641 tgcacttaga cagttagcta cagaagaagg atatgagact tttgtaatac cagatgatgt
    38701 aggtggtcgt ttttctgtat tgacagcagt tgggttactt cctattgctg ctgctggact
    38761 tgatattgat gctatgatga aaggcgcaaa tgatgctaga gaagcatttc aaaatcctga
    38821 cttaaaaagt aatgatagtt atagatatgc tgttgctaga acaattttac atagaaaagg
    38881 aaaagatgta gagttattag tgaactatga acctcaacta cattatgtaa gtgagtggtg
    38941 gaaacagctt tatggagaaa gtgaaggaaa agaaaataaa ggtttattcc ctgcttctgt
    39001 agatttctca acagatttac attctatggg tcaatacata caagatggaa aaaggctttt
    39061 atttgagaca gtacttaatg ttgaaaactg taagagaaat ataactataa gttcagagga
    39121 agtagactta gatggtttaa attatttagc aggtaagact gtagattttg taaatcataa
    39181 agcttttgaa ggaactctat tggctcatac agatggaaaa gtacctaatt tagtaataaa
    39241 tatacctcaa ctagatgagt ataattttgg atatttagtt tatttctttg agaaggcttg
    39301 tggaataagt ggatatttat tgggagttaa tccatttgac caaccaggag tagaagctta
    39361 caagaaaaat atgtttgctt tactaggcaa accaggttat gaaaaagaaa aggaagagct
    39421 agaaaaaaga cttaaataat tttaaatagt taaggtttaa ttaagattaa aataatataa
    39481 tgaaatcatc gaaaaggagt ttactcaaaa tagatgattt catttttttt atgaaaaatt
    39541 gggaaaaata tattttgata atttatatta tattaagata gggagtggta tgagatgtca
    39601 agaagaaaga aagggataag tttagtaata ttagttgcta taataagttc aatacttagt
    39661 agttttctaa caataatttt agttaaggac aatttagtaa gcaaatctac aggaagttct
    39721 acgccgatag ttgtaaatga tgatggtaag agtcaaaata tttatcaagc agttgcagaa
    39781 aaagctacgc catctgttgt aggtattaca acaactagtg ttgatactag taatatgttt
    39841 gcaataccaa ctgaaacaca aggtgtagga actggaatca tagttgattc aaatggatat
    39901 atacttacta attctcatgt tatatcagat ggtcaagcta caagtgtaaa tgtattattc
    39961 aatgatggtt caacaacttc aggtaaagta gtatggtttg accaacaact tgatttagct
    40021 atagttaagg ttgataaaac tggactaaca ccagcagaat ttgctgatag tgacaaagta
    40081 aaagttggag atatatcaat agcaataggt aatcctcttg gattagattt tcaaaaaaca
    40141 gttactcaag ggataataag tggattagat agaactatac aaacagaaaa aactaatatg
    40201 actggtttat tacaaactga tgcaagtata aatgcaggaa atagtggagg accattgtta
    40261 aatcaaaaag gtcaagtaat tggtataaac acagcaaaag cttctcaagc agaaggatta
    40321 ggatttgcaa taccaatcaa tacagctaag agtatagttg aagaagtaat caaaaatgga
    40381 aaatatgaaa aagtaacttt aggcataaaa ggtacagatg tatcaaatta tgaagcagca
    40441 acaggtacaa aattaagtac tgataaaggt gtttatgttg cagaagtaat atctggttca
    40501 tcagcagaaa aagctggagt aaaggtaggg gatattataa ctaaagtagg agatacagat
    40561 ataacaggta tgaatgattt aaataaaaag ttgtatactt tctcaaaagg tgctagtaca
    40621 aaaataacag taaatagagg cggaaaagca gtaacaataa atgtgaattt ctaaatatga
    40681 ccagaaagaa ggagactaca tggtaaagtg tagtctctat ttttatttaa tttttatagt
    40741 atatacatct aaattaatta aaatgat
//
LOCUS       DAMJVX010000017        39300 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMJVX010000017 DAMJVX010000000
VERSION     DAMJVX010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39300
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.018"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(66..917)
                     /locus_tag="PC407_000847"
     CDS             complement(66..917)
                     /locus_tag="PC407_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2496308.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDTIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKNFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
     gene            complement(943..1881)
                     /locus_tag="PC407_000848"
     CDS             complement(943..1881)
                     /locus_tag="PC407_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2496309.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            complement(1984..2634)
                     /locus_tag="PC407_000849"
     CDS             complement(1984..2634)
                     /locus_tag="PC407_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2496310.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            complement(2784..2972)
                     /locus_tag="PC407_000850"
     CDS             complement(2784..2972)
                     /locus_tag="PC407_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2496311.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            complement(3079..4263)
                     /locus_tag="PC407_000851"
     CDS             complement(3079..4263)
                     /locus_tag="PC407_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2496312.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            complement(4405..5322)
                     /locus_tag="PC407_000852"
     CDS             complement(4405..5322)
                     /locus_tag="PC407_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HDF2496313.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            complement(5344..6072)
                     /locus_tag="PC407_000853"
     CDS             complement(5344..6072)
                     /locus_tag="PC407_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2496314.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            complement(6268..6468)
                     /locus_tag="PC407_000854"
     CDS             complement(6268..6468)
                     /locus_tag="PC407_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496315.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            complement(6593..6973)
                     /locus_tag="PC407_000855"
     CDS             complement(6593..6973)
                     /locus_tag="PC407_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2496316.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            complement(7246..8604)
                     /locus_tag="PC407_000856"
     CDS             complement(7246..8604)
                     /locus_tag="PC407_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2496317.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTIMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            9052..9504
                     /locus_tag="PC407_000857"
     CDS             9052..9504
                     /locus_tag="PC407_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HDF2496318.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            complement(9661..10278)
                     /locus_tag="PC407_000858"
     CDS             complement(9661..10278)
                     /locus_tag="PC407_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDF2496319.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFIYIYMGKEFGAKRNNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDREQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            10827..13487
                     /locus_tag="PC407_000859"
     CDS             10827..13487
                     /locus_tag="PC407_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HDF2496320.1"
                     /translation="MKKILKRLCTGFLAFATVVTALPSSTVHASNTQYWTESKERVSI
                     VEQVMNDGSISSTFNEGHLTVEGEDAYCIDINTAFKNGYKTKADASTRMSADQIADVA
                     LSIEYVKQYTDIHSGISSKHAYLLRQLVVWQRLSVQPDWQWDNVRASYDEIPKAVQDE
                     VFAGAKAFVKENKGRYDCGGYIYSGEGQELGQFWAKLAVGNTKLQKTSTNANITDGNG
                     IYSIAGATYGVYSDKDCTKQLTTLTTDTSGNTEAVEVRATIVYIKELSAPAGFKIDKT
                     VYSLSVEVGKTATLKVSDTPKVTDTLIELFKIDMETQKSNPQGNASLEGAEFTWNFYA
                     GYYNKNNLPAQPTRTWVTKTIAEKDSDGAIHYITRLADKYKVSWDSFYTQDGKNVLPL
                     GTLTVEETKSPSGYLLEGTYMQADGSEEQIKGMYLTQITEDGDLAVLSGSNQYHVSDK
                     VIRGGVKIQKRDLETKDTKAQGGATLKDTAFEIISLNENAVLVEGKLYKKNEVLKTIH
                     TDIEGIASTSADLLPYGKYRLSEQKPPEGYLTDGAKPIDFEITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGSHKRLADVPFRITSKTTGESHVVVTDDNGQFSTASDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALLYDTYIIEELRCESNKGFKLIPPFEIVISRN
                     KVVVDLGTLTDEYEKEITIHTTATSKDGEKTILAGKDVTITDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIINDKRIENDYIFTADSETMEVKIEFTFDASNLGGKQLVTFEELYD
                     LSNPDEPIKVAEHKDIEDDGQTVTIKEVPETPTPEEPEKPTTPDTPTKTDSPKTGDNT
                     NILAFAIMMFVSAGGLAGTYFFKHRKMKKS"
     gene            13712..14035
                     /locus_tag="PC407_000860"
     CDS             13712..14035
                     /locus_tag="PC407_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2496321.1"
                     /translation="MELKYIVPNVAQTFGNLEFAGENKVDQQRINGRYTLVSRSYNLY
                     SDIQRADDIIVVLPAKAGEKHFKPEQKVKLINPRITAEGYNVKGRGFTNYILMADDML
                     PAESK"
     gene            14050..14433
                     /locus_tag="PC407_000861"
     CDS             14050..14433
                     /locus_tag="PC407_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2496322.1"
                     /translation="MRLTNGIVLDNEKTFGVLKFSALRHEVRVENEDGTTSDEIKRRT
                     YDLKCSVQERMIQVSIPADVPVKDFPYNAEVELINPVADTVANANFRGTTVDWYIKAD
                     DIVLKNKGGQAGNPQNNAPQGQQKK"
     gene            14529..14942
                     /locus_tag="PC407_000862"
     CDS             14529..14942
                     /locus_tag="PC407_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2496323.1"
                     /translation="MLDKIPNAEEMTALVGQSLYSVWNELCALIDEKYEMECLWNKGG
                     KAWNYEYKYRRGGKTLCALYARENCVGFMIILGKDERLKFDTYRDNYSKEVQRIYDET
                     KTYHDGKWLMFEPTDTTLFDDFVKLLRIKRKPNRK"
     gene            15049..15507
                     /locus_tag="PC407_000863"
     CDS             15049..15507
                     /locus_tag="PC407_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2496324.1"
                     /translation="MKLKNPMLVVTDIDKSVEFYKKVFGLYVIMDFGANKTLTGGLAL
                     QTSETYKEFIGTSDISFGGNNFEVYFEEEDFDKFADRLKEYDIEYVHPIIEHSWGQRV
                     VRFYDPDKHIIEVGENMKIVCKRFLNSGMTPEQVAERMDVPMKFINACMR"
     gene            15532..16074
                     /locus_tag="PC407_000864"
     CDS             15532..16074
                     /locus_tag="PC407_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2496325.1"
                     /translation="MKGFERKNQLFSLCGLNCGLCPMLLGNYCGGCGNGNQSCRIAKC
                     SLEHGQIEYCYECKQYPCEKYQHIDDYDSFITHKRRKADLERAKNIGIEQYNLEQQEK
                     TQILSYLLSNYNDGRRKTFFCVAVNLLELSELQEAMKQIQSNDELTLLPFKAQCLYVV
                     EVFQKIAERRNIKLKLFKKK"
     gene            16110..16637
                     /locus_tag="PC407_000865"
     CDS             16110..16637
                     /locus_tag="PC407_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HDF2496326.1"
                     /translation="MTNGGIPLETVELRFQYTQSEYIKAERQYLISSKTIHKYDIALV
                     AVFLLLSVVYMLFSSFSVFSILIFGLIIVVTALGSYLYILMPILKFKQTAKYHEEYTL
                     VFSKENIKFKTQSIDSEVKWDIYSALWESHDFYYLIQAPRLYTLIPKRIFKDLNEKQL
                     FEEIAQSRVKTTKHI"
     gene            16747..18141
                     /locus_tag="PC407_000866"
     CDS             16747..18141
                     /locus_tag="PC407_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2496327.1"
                     /translation="MRMILNKGHRIRARDKNLVYHFSIGTLLFVFVAVLLLLNIKQLM
                     RTDWEHFSLLDNGLTLSPYNFITILIATGICALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMENGLLHIHCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRISIDEVKAENGCLRLMKNLV
                     WEYDVLPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEDMIDCVNAFYEGMVQRSEEMKQHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAFVRQ
                     DGTATCEAKGDGTD"
     gene            18421..19542
                     /locus_tag="PC407_000867"
     CDS             18421..19542
                     /locus_tag="PC407_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDF2496328.1"
                     /translation="MAGISREHLSRIEAGKVALTEDMKHKLLEAVEKFNPDAPMFLLF
                     DYVRIRFPTLDIQHVIKDILKLNIDYMLHEDFGHYKYTEHYYIGDVFVYTSQDKEKGV
                     LLELKGKGCRQFESYLLAQERSWYDFLMDALVDGGVMKRIDLAINDRTGLLDIPELIE
                     KCKNEECISKFRSFKNYGSGELVKHNEANKDGMGHTLYIGSFTSEVYFCVYEKNYEQS
                     SKLGIPIEEVPIKNRFEIRLKNEHAYYAIRELLTHYDAELTAFSIINQYIRFADKEAN
                     KRKSDWKTNARWSWFIGEGRPPVKLTTKPEPYTLQRTLNWLQRQVAPTLKMLKQIDAG
                     NQMDYLKTIEQQAKLTDKHKQIIRQQTASVDELIEKEKE"
     gene            19549..19683
                     /locus_tag="PC407_000868"
     CDS             19549..19683
                     /locus_tag="PC407_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDF2496329.1"
                     /translation="MWELIKDLLLVSLGMGIGVVLMCIMSVGKEADRHMIELKESEEK
                     "
     gene            19684..19905
                     /locus_tag="PC407_000869"
     CDS             19684..19905
                     /locus_tag="PC407_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496330.1"
                     /translation="MNFGQNLYTWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALVAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            20225..20863
                     /locus_tag="PC407_000870"
     CDS             20225..20863
                     /locus_tag="PC407_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HDF2496331.1"
                     /translation="MEYKYTKIDLNKWNRGKLFQSYIDNMRIVMSLTVDIDVTKFIEF
                     SKKHDLKFYPAMIWAVSKVVNEHDEFKYSWDNNGNLIKWDYISPSYTEFHKEDENFTK
                     IVTDFSDSIFEFHKRFILDREKYKTERAFVENQSLNFFDVSCLPWVKYNHFDVHVFDE
                     GKFLAPVITWGKYELEQERYIMPLTMNIHHAVADGFHLSRFFNEVQELINSI"
     gene            20940..21128
                     /locus_tag="PC407_000871"
     CDS             20940..21128
                     /locus_tag="PC407_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496332.1"
                     /translation="MKDIFSDMQASIGCTYISDLPHHKRMVWQEMKRLNLADYPQKQL
                     EDFAGYVFGVPYTSIAEA"
     gene            21149..21646
                     /locus_tag="PC407_000872"
     CDS             21149..21646
                     /locus_tag="PC407_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2496333.1"
                     /translation="MNEMRVCIESRLHTSEEIRSAWFTLPIDEEELEEQIGVPIDSED
                     YHIVEKELPFADEVGEDATIEKLNDLYHTFESLPADLQEDYGELMCYFTSLDELHQHR
                     HDVIHYSWCKNMADVARHLLDNDPAFSAIEERLTRYFDYEAYGQYLDDNGEFIETDHG
                     IYELP"
     gene            21662..22165
                     /locus_tag="PC407_000873"
     CDS             21662..22165
                     /locus_tag="PC407_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2496334.1"
                     /translation="MVEDMRVYIANLGKYNEGYLVGDWFSFPIDEEDVAERIGLNERY
                     EEYAVHDTENFPIEIGEYISIEELNEMYEMICELPDYITDSLDEFVSNYGSLEELYDH
                     RDDMILYAGCEDMTDVAYYFIDELQALGDIPPSLQNYIDYEAYGRDLSFEGTFIETRY
                     GMCEIPW"
     gene            22278..22667
                     /locus_tag="PC407_000874"
     CDS             22278..22667
                     /locus_tag="PC407_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2496335.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDLPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITFALRPKITY
                     AGKAVKLDKQVLHEAITAVRSVSYVPD"
     gene            22654..25086
                     /locus_tag="PC407_000875"
     CDS             22654..25086
                     /locus_tag="PC407_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2496336.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSSRSIQEQSKKLVTGRLKDVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTDQSMNLKSVKQSAFLTFKEFIHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLDKNDFGYLIEHIYGRDGVAYEDYEYSLPKKKLKKE
                     TLITHYDLIRPTRCLVEESQRHLRLEHEDSESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALSTVRNKKKELKDLDNHAYQSGSETSSNVVDALDSVD
                     ELETDLDQSRESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYIGYSVDTGRNVYLQPS
                     LASQGVKDTVTNALASAFVESLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDRENAGLLDPFVIMQNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRAVTQSDQRGLLHVIEELRKEDTPISCNIADHIDSFTDYDFAHLLFSDGTV
                     QNAISLDNQLNIIQVADLVLPDKDTTFELLSVSMLIVISTFALDFIHSDRSVFKIVDL
                     DEAWAFLNVAQGETLSNKLVRAGRAMNAGVYFVTQSSGDVSKESLKNNIGLKFAFRST
                     DINEIKQTLEFFSIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFEELLHAF
                     DTRPPIQRNEVE"
     gene            25086..27221
                     /locus_tag="PC407_000876"
     CDS             25086..27221
                     /locus_tag="PC407_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2496337.1"
                     /translation="MKERIKSVFTRKKIFRFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINTENLYSKYPLSNYQLDFYADNNWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     RLYLSNATGYVVQEAYKLDFINDMADSIGRSIQTLAGVTQNGFSTSGFYVGFLLLIIL
                     VVGIYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKVNDFSSDISTAS
                     LDLGTKIMLPDSDSKGKDSVDLIRDSLFSIQVQQPWLLLQFGNSGIEEIGADRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTILQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVYFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRLARAVSAGGVAGAVAGGVVASSKSTRQNGTKNNRGNTSSSMGQK
                     AGSAVGAVLDTKNKVKDKATAVKENIKDMPTQTAYAVHSAKEKAKSSVSDFKRGVVQE
                     KENRQSGRLKKQQLHRQNIADKRMELQKAQESRQAGKKTDGSATSGATRPHERPATTS
                     KVNVEKQQEVKRPAIASTVKNDEPIKKNVVKERPLSSGDTSAKTVNQETTRQNYKTDR
                     VTKTTVQKHTQQNTEKNKRTVFKKGQKKK"
     gene            27218..28225
                     /locus_tag="PC407_000877"
     CDS             27218..28225
                     /locus_tag="PC407_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDF2496338.1"
                     /translation="MKLRHIAIVGSLFSILFSLVLFFGVLISAEDDDGGNGYSSNYVG
                     MNLSAEVLKHQPMVEKYAKENGISEYVNVLLVIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGISDINVVIQSYNYGGGYVGYVAGNGKKHMFNLA
                     ENFARDKSGGQKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLVVNQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYNKAGVSLPRTAQNQYDATQHIPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNTQMYHAGDPIGYADLNNAYWQQYLICAGRIK
                     Q"
     gene            28241..29134
                     /locus_tag="PC407_000878"
     CDS             28241..29134
                     /locus_tag="PC407_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2496339.1"
                     /translation="MFKKKEKPVKETKVRTMKVGTHKKSVIALWVVLIASVSFGVYKN
                     FTAIDMHTVHETETIQLRLNDTNGVENFVKNFCKSYYTWDNSKEAIEARTQAISNYLT
                     KELQDLNLDTIRTDIPTSSTVTNVLIWDIKQSGTNDFIATYEVDQQIKEGEQTSNVKA
                     TYTVKVHVDKDGDMVIVQNPTIAPAIEKSDYEPKAQEADASVDADTVNDATAFLETFF
                     KLYPTATEKELAYYVAGNVLEPIGRDYLYSELVNPMFTDDRDNVKVRVAVKFIDNQTK
                     AMQVSQFELVLHKDSNWKIIM"
     gene            29347..30204
                     /locus_tag="PC407_000879"
     CDS             29347..30204
                     /locus_tag="PC407_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2496340.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERNSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPAWSHRFMGLIQLGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     AIQCPVILITAKGDPLFRFDYTNKVFDLIHVPKKELLTLELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            30282..30863
                     /locus_tag="PC407_000880"
     CDS             30282..30863
                     /locus_tag="PC407_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2496341.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFSLYGLKKVTVDDLAAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTVKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEGLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMVQNLLVNGIIQQVVAE"
     gene            30967..31812
                     /locus_tag="PC407_000881"
     CDS             30967..31812
                     /locus_tag="PC407_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2496342.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KILTGLNTSYKGSVKVAGTEIREHTNRFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     DKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNNRLMKDIILAEKKRGKTIIITTHNMYDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIHYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            31809..32492
                     /locus_tag="PC407_000882"
     CDS             31809..32492
                     /locus_tag="PC407_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2496343.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALTGHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKVSEYILSKTIALIAISTI
                     IAVILGFVSGSNHLLGIAVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFV
                     PPIVGLFVKLPDIFRFFPFTACMNLITGKSILLSFDMVLVIATLIILYIVARHTVKHM
                     WRSLGGVKL"
     gene            32489..33187
                     /locus_tag="PC407_000883"
     CDS             32489..33187
                     /locus_tag="PC407_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2496344.1"
                     /translation="MKRILSSTIQVFKQIKSDPMMFAACFTPFVMGALIKFGIPFLDG
                     ITDFSLQTYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLMVALVW
                     YYFLIKRLFRKLAG"
     gene            33199..34110
                     /locus_tag="PC407_000884"
     CDS             33199..34110
                     /locus_tag="PC407_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2496345.1"
                     /translation="MEKGRLLQNENINKAIQVSKITLIINLGLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVLSTIVVMVGIKISEKQPDKEHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVSSGIALTAAVLSIVIKEWMYWYTRSAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASIVICGCILKAALDIFKESINKMVDHS
                     CDNATETKIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELPYSP"
     gene            34163..34303
                     /locus_tag="PC407_000885"
     CDS             34163..34303
                     /locus_tag="PC407_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496346.1"
                     /translation="MEQYWWIIIVMVLIVVGLLKIVIMKKYNQRRNSNNRSKNSEDDK
                     MY"
     gene            34418..34609
                     /locus_tag="PC407_000886"
     CDS             34418..34609
                     /locus_tag="PC407_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HDF2496347.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            34633..34863
                     /locus_tag="PC407_000887"
     CDS             34633..34863
                     /locus_tag="PC407_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496348.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            35129..35419
                     /locus_tag="PC407_000888"
     CDS             35129..35419
                     /locus_tag="PC407_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496349.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELRRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKNVTMY"
     gene            35419..35553
                     /locus_tag="PC407_000889"
     CDS             35419..35553
                     /locus_tag="PC407_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2496350.1"
                     /translation="MYYFLEMSDTEIADIMNYTREAVFKHRHRALETMKEILNEKTRQ
                     "
     gene            35534..36715
                     /locus_tag="PC407_000890"
     CDS             35534..36715
                     /locus_tag="PC407_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2496351.1"
                     /translation="MKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYSWKLVP
                     TDKTPAGKRDDISLREKEKQIKKDLDDGIDTVGGKMTVCQLYEKKNNQRKNIKRNTEL
                     GRQYLVNALENDPLGMRAIDTVKQSDAKEWAIRMNGKGYSYKTIDNYKRSLKASFYMA
                     IQDDCIRKNPFNSKLSDVLEDDTEAKVILTPEQEEKLLAFMETDNVYSKYRDEVILLL
                     ETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISKAKTKNGKRELPLTERAY
                     QALKRILKNRGKAQPLVVGGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHKDKL
                     PNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELERLA
                     S"
     gene            complement(37055..37867)
                     /locus_tag="PC407_000891"
     CDS             complement(37055..37867)
                     /locus_tag="PC407_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2496352.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRIL"
     gene            complement(37943..39178)
                     /locus_tag="PC407_000892"
     CDS             complement(37943..39178)
                     /locus_tag="PC407_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDF2496353.1"
                     /translation="MGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITSGVTGMF
                     CHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLRGSLASLLEVESGSNDPIAY
                     MLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFYTIF
                     ITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIMLFF
                     MLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVGLRG
                     AASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTPVLK
                     TFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGSTII
                     KTGDILVLSGDRIEELIQL"
BASE COUNT        12762 a         7015 c         7046 g        12477 t
ORIGIN      
        1 catttttaca gtatatacat ctacatatta taaaaagcaa aattatattt tctaaatatt
       61 ttatgttaga ttataatatt aaattctatt atctctggta tattaaaaaa tctaaatggt
      121 aactttgtac ttccaagacc cgaactcaca tataaatgat ttccatttat atcataaaac
      181 cctttagtat atttcctccc atatggagga gtaactaatg ctcccttaat aggtaatctt
      241 atttgaccac catgactatg cccagataga actaaattca ttgtatcttt actaagcata
      301 tctactaaat ctggttcatg tgctaacaat aaattaaaat ttttattatc tatctgactc
      361 tccagatatt ttatattagg atttccattt aatatttcat caacacctaa tatattaata
      421 tatttatcat cttttaattt tattttttta ttttcattta ctaaaagatt gaagttggaa
      481 tttttcataa tctgcctata atacctagga agtttataca tataatcatg attgccaaat
      541 actgaaaatt ttcccaacct agcattcata cttgataaaa tagttgtaat ttcatctaca
      601 cttggatttt tattggagta atcaattaaa tcaccagtaa atactattat atctggattc
      661 aaagtgttaa tcttattagc tacttttttg agattcttag ttgaataaaa actacctatt
      721 tgagtatctg aaatttgaac tacttttaca ctttcagatt tactctcaat tgtatcattt
      781 tttatcaatg ctccattttc acttatatta tattttttca caattaatag actcggctct
      841 atcgcaactg agtatataaa aatcaaaata aatattaaga taaaaaattt taaaaaatac
      901 ttaatttttg agttcaaaat atctacctac ttatttattt ttttaaacta tttgcattac
      961 agactcaact ttgttcattt ttctgtcatg catttttggc ttatttgctg aaaaccattt
     1021 atatgttgct gtaagacctt cttttagtaa cacatttggt atataaagcc catgttcaat
     1081 aagcttattt atatcaagat taaagtctat atttctaaat gggaaatacg ttctttctct
     1141 aaactctact ttttccatat ctacatattt tattataggt tctttgccaa taatttctcc
     1201 acatgtatat ataaggtcat cccaagaaat taactgtgga tttgtcacat tataggcctc
     1261 tcttacatga ggatttttca ttatgctttc caataccttt accaaatccc caatatatat
     1321 aaattgatta ctaacttgtt ttccttttgg tactggtatc ttttcatttt tttctatttt
     1381 ttcaaagaaa taatactctc tatataaatt attattttca ccatatatgt aagttggtct
     1441 aataattata tatggtatag ggctatttat aataaaatct tcagcctctt ttttatttaa
     1501 cccatatttt ccccagtttt cattttcacc tttctcattt tcttctttta tatttcttcc
     1561 actatcttta tatactgctc ctgctgacaa tactatatac ttttttaagt tatccatgct
     1621 tataaaatca atcagattac ttacatcttc ttttgtatat gctgtcatat catatatgta
     1681 atcatatttt cttcctgtga taatgttttc catatctttt cgaacttttc tatcacaaat
     1741 tagatgttct ttaaaaccat tataatctat ttccttttta ccatttgtaa gtatatcaat
     1801 ttgataccca catttaatca agcgcttagc aagtgcagaa cctatgaagt cgcttccacc
     1861 cataattaaa atagatttca ttttaagtgc atcctcctct tttttaaatg acttccttaa
     1921 ctataatatc atatttttaa atatgcgaca ccattttgta atatttttgt ctataaaaga
     1981 tatttataac aaaatatctt ttatagactc tacattttcg acaatataag taggttcact
     2041 ttcactaagc tcttcaaaag aaccataccc atacaatact ccaatagaat ctatacctat
     2101 ttttttagct ccaattatgt catactttct atctccaacc ataataactt tatctttatc
     2161 ttttacatta cataggtcta gtacatattg tataacttca tttttattca ctcttgtacc
     2221 atcaagattg ctacctgcta tatatttaaa atatctatct atatcaaaat atctaagtat
     2281 agtttctgca aaaactgttg gcttagaagt tgctactaat aatattttac catttttata
     2341 gagcatttct aatatctctt tcatattctc ataaatttta ttttcaaata tccctttatc
     2401 agcaaaatat tctctatact tttcaacagc ttctttagct tttttatctt caaatttata
     2461 atactccttg aaagtatcat gtaatggtgg tcctataaat tggtctaagt tttccaaatc
     2521 ttcttttatg ccaaagctat ttaaagaata ctgaatagac tttgttattc cttctttagg
     2581 gtctgtaagt gttccatcta aatcgaataa tactatttca taattttttt tcatatttca
     2641 gtcctccata tttgtaaaaa gtaatatctt acatattaag tttatctatt ttaaaaagct
     2701 atagtaatag aaatttctaa tactatagct taagtaccca attaaatctt atatatttat
     2761 tttaaaaata aatttaaaca gctctattta actgttatag ctgaaactgg acatccttct
     2821 tcggcttctt tacataaatc ttgtaaatct gctggtattt ctccttcaat tgctactgaa
     2881 tgcccttcat cgctcatact aaaaacgtct ggacatgttc ctatacatgc tccacaagct
     2941 acacatacat ccttatcaac aaatgctttc atactaaaat acctccccta aaattattat
     3001 tttttaattg ttgtttcctt agatatttat ccaatatata ccaatttaaa catatattta
     3061 ttattttatc actattgttt agtttattat agtattttcg tatccatgtc tataaacttc
     3121 actgcttttc atccatgatt ttacagtata ttgcaatctt aattttttat ttaaaacaag
     3181 ttcattatta ttgtagtctt ttataatatc attacactcc tccattgtga atattgtaaa
     3241 atcaccttct cctccaatct ttattctgcc tttatttcca tgtaaatcga aaatctttgt
     3301 tgggtattca gttacttttt ctatacactt ttctagggat atcccaagtt ccataatttt
     3361 atttataaca tttaaaagag aatagtttac gtctttcatg ttttttatat gcatatcagt
     3421 acttattagg tcaggctcta aaccttgctt tattgctttt ttaaaaactt taaaatcaaa
     3481 ttggtccact ccatgacata catcgaacaa tactcctcta ttctttgcat ttattactga
     3541 ttttcttaat gtgccagatt ctttaaggat tccatttgta ctatttccat atactggtgt
     3601 aaccacatct ccttttccca aaatattcat tacttcatct atatatgatg gatactctcc
     3661 tatatatacc actaagggta aatttaattt tttagcaact tgctttgatt tcttaattat
     3721 atctatacca ctttcattag attgaaattt actcgctttt acttttatac cctttatctt
     3781 ttctatattt ttttctacaa ttgactcaac cttgtcaaaa tttataacat ctaaagaagt
     3841 tgattcattc aatatattta gtccctcatt tgtaacattt aaaaaagaat atacttctgt
     3901 tttacactca tccatatact tttttttaaa atcatagtag tttgagggac cagctgtacc
     3961 tgcatcaaat ataactgtag aacctttttc aattccaatt gaatcagaat ccatacctgt
     4021 aggtaatttt ctcttataac aatgggtgtg aacatctata aatcctgggg aaatatacat
     4081 attatcggca tttattatct cgaactcttt atttgtaagt aatttttctt ttcttatttt
     4141 ctctttaagg ttcaatccaa tagcatatat tttcccatgt aaaaccaaga tatctctttt
     4201 ttcaaatttg ataccatctg ttggagatat tattgtccca tttcttataa gtaaattttt
     4261 catttattcc acatcctaat tacaatataa tttctctaat agcttttctt acataaagaa
     4321 aataagccta aaaataaaaa tattttagac ttattcacta tattatacta tattaattat
     4381 attaaattaa ataaaatctt taatttactt taagaagcaa ttaagtattt gttgctcggc
     4441 ttcttcttca gtaagaccta atgtcatcat ctttagtatt tggtctcctg caatctttcc
     4501 aatagctgct tcatgtacta aaagagcatc tggatgattt gcagcaattt caggtatagc
     4561 actaatttga gaatttccca taattattga atcacattga acatgtgcag aacaaaggga
     4621 atccccaata acacaaggat taaacttttg cttagaatta tcttgcccaa ctgtacgaga
     4681 aactacttga gcagaagagt tttcaccctc taagataacc ttcatattag attctgcaac
     4741 ttgattacca tgagttaata atttctcaaa tacaactatt ttagctcctt tacctaggtt
     4801 tgcatccatt tctcttttag ttgaatctac acccttaatt tgaattgttt ccatttcaca
     4861 atatgaatcc tcatccatat tgacaatagt aactggattt aaaattctct ttccttctcc
     4921 atcaccttca ccataatgtt tttctacata ttttaggcga gctcctttac gtaagaaaaa
     4981 tctatgtata ccattatgtt cactatcatc acaaccacag ttatgtatac cacacccagc
     5041 aattataaca acatctgcat tttctccaat ttcaaaatca ttatatacca tatcctgtac
     5101 accagcctct gttactatta ctggtatatg tacattttct ccttttgtat ttggctttac
     5161 tataatatct attccaggtt tatcagtttt aggaattata gttacatttt cagttgaatg
     5221 tctctctact ccttgcccat ttttacgtat attgtatgct cctaatggag ttccacgaag
     5281 ttcagcaact ttttctaaaa gatttaaatc tattttgttc atttaatttg ccttcctttc
     5341 taattaattc atgtctttca ttaattcaca acttgaatta acttgacaca tgatttcagg
     5401 taatatagcc tcttttgttc catggctttt tactgaacca tcttgtagaa caattatttc
     5461 atcagctaac tctaatattc tttcttggtg agaaattatg attatagtct gattagactc
     5521 ttcatgtatt ttcttaaaac tttcattgag ttttccaaag ctccaaaggt ctatccctgc
     5581 ttcaggttca tcaaatatag aaacctttaa atctctagca aaaagagttg ctatctcaat
     5641 tcttttcatt tcccctccag aaagagagtt atcaacttca cgatttaaat agtcttttga
     5701 acacaatcca acatctgtaa ggtattggca acatacatca gtagacaatg gttttccatg
     5761 agcaagtgta agtaaatttt caactgtcat accttttatt cttggtggtt gttgaaatgc
     5821 gtatcctatt cctttttttg ctctttctgt aatactaaga tttgttatat cttctccatc
     5881 taaaattatc tgaccactcg tagctttttc aattcccata ataagcttag caattgtaga
     5941 ttttcctcca ccatttggac ctgtgataac aataagtttt cccctctcaa atgaaaggct
     6001 aacatcattt attattccta gttcctcatt gttagactca acattaaagg aaagattttt
     6061 tatttcaagc ataatacccc tcttttctat aaaatattac attaatatcc attcttttat
     6121 tatatcagta atttttatat atcagcaact ctaagcagat aaaatgtata ttaaataata
     6181 aaacaaagtt attgaaaagt aaaaaagatg agttttatgt aaactcatct cagaaattta
     6241 gtatatcaac ttatatgtat ttaaaattta accttctata tactctaata ttattttttg
     6301 attatctttt aacataattt cttctttttt agattctatt ccattctttt ttatctttac
     6361 ctcagctaca aaggcatcat taccttcaac catttctaat agttcatacg cttttttacg
     6421 acctatttta gttgctatat cttttagtac tgccattttc ttaaacattt tatcacctct
     6481 actcaaaatt tacacttaat ttttaattat ataattctaa tcctagacta gtttataata
     6541 attattgtct ctaattatat cagatattga gtaaatttaa tactttgtaa aattatttat
     6601 cctctctgac taaataatca tttccatctt ggtctttaaa agaaaacatc cttccatatg
     6661 gcatctccat taattcccca acttcaacct cgttatttgc catttgttca taagttccat
     6721 taatatctct tgtacttaaa attattgatg gatgagatac atttatctct ttgttttgct
     6781 tcatcattag atttttatca tacaacacaa atgttgtaaa ttcattttta cttggtgcta
     6841 cttccaccca tttcatattt ggacccattt ccttttcata agttactaca aagttcaatt
     6901 tttctgtcca aaacaacttt gcatcctctt gattatttac atagagagtt atttttccta
     6961 ttttatttat cataacaata cccaccttta ttattaaata aatacctaat attttttata
     7021 tttaataata tacccatata tattattttc tatagatata tatttctaat atttatcgcc
     7081 aaattatgta attctgctca atatataatt tataatatcc atactattat agcctttatt
     7141 tatgtaattt aataacctta tatatcatac ttataattat gataataact acataaaaaa
     7201 tcacttctaa atagagttat ccatctaaaa gtgatttcat atattctatg caactttgtg
     7261 ttttagccac tttgttcctc ttaatcttaa acaatataaa gtccctctaa caagccaatc
     7321 agtatacata cctatccaaa ttcctaacac tccaaattct aatacaaccc ccaatacgta
     7381 acctgtaaaa attctaaaaa tccacatacc tattactgca gtcatcattg tatatctagt
     7441 atcaccggct ccctttagcc ctgctgataa tacaaaggat ataggccata ttaccatagc
     7501 taaacaatca cttcgaatta attgtgttga gatgtcaatt acactagcat tttgagtata
     7561 catacttgaa atccagggtg caattggtat aaatatagct cctactataa ataaacaaat
     7621 tgttgcaaat ttagttaaat ataataaagt actcttagct cctttaatat ctccacgtcc
     7681 tatatactgt cccaccaaag ttgtagcaga caaagctaaa gcattcccaa ttacatttat
     7741 tatggatgca attgacattc ctattgcatt ggatgcaatt gctgatgttc ccatagtaac
     7801 tataaatact tgaacaatca atttacctgc attaaaaagt agttgttcaa ctccagctgg
     7861 tatccctata ttaaatatag acttttgagt ttcaacatca atcttaaatg gaaatatttt
     7921 acccatagat ataaattcat ttcctttaaa taaagctctt ataattacta tacatccaat
     7981 tattctagct atagcaatag atatagctgc acctgttatg ccataggatg gtgtttgcaa
     8041 atttattcct agaggattta aatcaatacc atatataagt atatatccta atgttacatt
     8101 tactatattc ataaacatag tcacatacat aggtttcttt acatctccac accctcttaa
     8161 aataccattt gctatttgtt gtgtcgcaat aaatggatac gtaaataatg taaactctat
     8221 gtataatttt gcatttgatt ttaccaattc ttctgctgaa ccataaaata aattaattac
     8281 accagttcta aaaacccaca tcaatattgt tattgtaaag gataatatta ctccagacac
     8341 tagagcctgc ttaatagttt cattcacctt ttcactatct ttttttccta tttgttgagc
     8401 tacaacgaca gtagctccaa cagataatgc agcaaataca gatataaata tattgtttat
     8461 tgaatcaacc attccaactg cagatacagc ttcttcccca atatgacctg ccattattgt
     8521 attaaatact cctaaaagca tgacaaacgt ctgctctatc attataggaa ttgctaactt
     8581 tataacattt ttccttataa acatccctat tcctcttttc cctctttata taaatttaat
     8641 ttttaattag tttatcgaac tgtaaattga caatgcaaag tttagaattg tattgttcgc
     8701 attataatat actgctttca aataatataa atcttgttta atatcatttg aacacagtat
     8761 atctttataa ttatcttatg tttattattt tttatggata ccacatttaa aatatttatt
     8821 aatttaatat aaatattttc cgttattatt aaaagtagct atatttcgtt aatattgtat
     8881 cactatcgtg tagcaataac aataagtaca ttttatatat tttttataat attatttaat
     8941 catccaaact tttaccactt tttcaatata taaaatatta cactttagat gtaacaaaat
     9001 cgcatccatt taaatattgt attttataga ctaattttag gatgtgatac tttggttaat
     9061 ttaaatgcaa ataatctaaa tgtaaatatg gtttatccta tttcttccac agcacccata
     9121 cgtttcagaa gatatactgt ttcatattta gaaaacatgg aaaatattta tttaactata
     9181 gcaacatact ttgatacact tggtttaggc acaatgaata tcgacaaagt ttatggtcaa
     9241 tgggcttggt ttactgataa tatatatgat ttaaatctat ttgtttttgt aggaaattat
     9301 ccttatcaaa ttgctaaaaa cagatatgat acatttgtag atattttgcc agtagctatt
     9361 acttctattg tcaatggaga tagagtattt ttaatttcaa atccttgttt gttaaattca
     9421 aaaattacag ttagatttat atctagtcat cctactctta atagaacagt tgattatggc
     9481 aatattagag attttatttt ttaaaactgc aacatgatgt gctattaaaa aataccctaa
     9541 atttaatgat attattaaat aactagaata tttttaattg gcacatcatg cctagctatt
     9601 ataaattcat aatattattt tacttaagat aagtactgta ttttatctac ttgtatattt
     9661 ttatttacct agtttctcag ttctatatcc tatttcttta ttagctagct tataaccttc
     9721 ttgtatcatt tcttctctcg ttaatgcact ccctaataaa ttgtcaatac tcagttgctc
     9781 tctatcacta aaatacttat ccaataatga ttcctctata tcttcctgat atgccaaatt
     9841 tccttcatgc attatatatt ttacatcgaa accttcttta tttaatactt ttcccaccaa
     9901 aatacttcta tgacatctta taggttcttg catagctcct aataaaacta tcctataacc
     9961 catttgacaa ccctttttta atctttctat tccgtttaaa aaaatatctt cttttgctac
    10021 tttctcaaaa tctgcataac cttcttgcgt atatacatct ttattatttc ttttagctcc
    10081 aaattctttt cccatgtata tatatataaa tccggaattt ctaagagatt cgttaaatgc
    10141 ctctttattg tattgaatat tatatttaga atatggagtt tctctaatgt caactacaca
    10201 atttatgtca tattttttta tcatattaat aagcttatca tatggataat ttgaatgtcc
    10261 tattgcgaat atttccatat aaataccctc ctattctttg aaattagtat ctatatatgt
    10321 agatacccga tttacttaaa attaaataaa gtattacttt aaatacccaa tatctttttt
    10381 tcttgatact gtatattttt ttactttata tccttaaaaa aatatacaac tttatatata
    10441 aaacatcaat atttatatat attttgtata ttaaatacaa ttatttataa aatctttgat
    10501 aaaaatttat aaatagagca tctttagaaa actattaaca gaaaaacaca ctaaaaattt
    10561 gattatccgc taaaacactg tatctatcgg cattatcaag gagctaaaca catcaaaata
    10621 ctactaattt actacgattg aatgagcctt gatacgtcct atttcccaga tatgcaaaga
    10681 tacggtacag cacatcagta ttgaaacaag aaaaattgaa tacttcatag actacggaac
    10741 gcacaatatc agcgttcctt ttctatttcc agccgttcct agtggacggc tattttacta
    10801 ccaaaatgaa aggagcattt agaattatga aaaagatatt aaaacgattg tgtacaggct
    10861 ttcttgcctt tgcaactgtc gttaccgctt taccgagttc aacggttcat gcgtctaata
    10921 cacagtattg gacggaaagt aaagagcgtg tcagtatcgt agaacaagta atgaatgacg
    10981 gttctatcag ttctaccttt aatgagggac atttaaccgt tgagggtgag gacgcttatt
    11041 gtatcgacat caacacagcc tttaaaaacg gctataagac caaagctgac gcaagtaccc
    11101 gtatgagtgc cgaccagata gctgacgtcg ccttatctat tgagtatgtg aaacagtaca
    11161 cagacatcca cagcggaata agcagtaaac acgcctacct attaagacag cttgttgtct
    11221 ggcagagatt aagtgttcag cctgactggc aatgggataa tgtgcgagct tcctatgatg
    11281 aaatcccaaa agccgtacaa gatgaagtat tcgcaggagc aaaagccttt gtgaaagaaa
    11341 acaaaggacg ttatgactgt ggcggatata tctactctgg cgagggtcaa gagttagggc
    11401 aattctgggc gaagttagct gtcggaaata ccaaacttca aaagacttcc accaatgcca
    11461 atattacaga cggcaacggg atttattcaa tcgctggtgc aacatacggt gtgtattctg
    11521 ataaagactg cacgaaacag cttaccaccc ttacgactga tactagcgga aatacagaag
    11581 ccgttgaagt aagagccact atagtatata tcaaggaact ttctgcacca gcaggattta
    11641 aaattgataa aacagtttac tctttatctg tggaagttgg aaaaacagcg actttaaaag
    11701 tatctgatac accaaaagta acagatacct tgattgagct attcaagatt gacatggaaa
    11761 cacagaaatc caatccgcag ggaaacgctt ctttagaggg agcagagttt acatggaact
    11821 tctacgctgg atattacaac aaaaacaatc ttccagcaca gcctacaaga acatgggtaa
    11881 caaagactat cgctgaaaaa gacagcgacg gtgctatcca ttacattact cgtttagctg
    11941 acaaatataa agtatcttgg gacagcttct atacgcagga cggtaaaaac gtacttccat
    12001 taggaacact aactgttgag gaaacaaaaa gtccgtcggg ttacttgtta gagggtacat
    12061 atatgcaggc tgacggtagc gaagaacaga taaaaggaat gtatctgaca cagattacag
    12121 aggacggcga ccttgccgta ttatctggaa gcaatcaata ccatgtttct gataaagtca
    12181 ttcgtggcgg tgtgaaaatc cagaaacgag atttagaaac caaagacacg aaagctcaag
    12241 gcggtgcgac tttgaaagat accgcctttg aaatcatttc cttaaatgag aatgccgtct
    12301 tagttgaggg caagttatac aagaaaaatg aagtgttaaa gacaatccat accgacattg
    12361 agggtatcgc ttctacttct gctgacctat taccttacgg aaaatacaga ttgtctgaac
    12421 aaaaaccgcc agagggatat ttgactgacg gtgcaaagcc gattgatttt gaaatcacag
    12481 aaaatggaaa aatcgttgat ttgactgacg aagcccactc tatctataac cagattaaac
    12541 gtggcgatat tgagggcgta aaaattggtg caggttcgca caagcgtctt gcagatgttc
    12601 cgtttaggat cacaagcaag acgacgggcg aaagtcatgt tgttgtaact gatgataacg
    12661 gacaattctc tactgcttct gattgggctt cccacaagca caataccaat gcaggaaaaa
    12721 caagcgagga cggtgtgtgg tttggtactt ctgaaccaga cgacagcaaa ggtgcgttgc
    12781 tttatgatac ttacatcatt gaagaattac gttgtgaaag caacaaaggt ttcaaactta
    12841 ttccaccgtt tgagattgtc atttccagaa ataaggttgt ggttgattta ggtacgctga
    12901 ctgatgaata cgaaaaagaa atcactatcc atactactgc taccagcaag gacggcgaaa
    12961 agactattct tgctgggaaa gacgtaacta tcactgatac agttaaatta gacggtctta
    13021 ccaaagggac aaaatatcag ttgaaaggct ggcagatgtt aaaagaagaa aatgcagagc
    13081 ttatcatcaa tgacaagcgt attgagaatg actacatctt tacggctgac agcgaaacta
    13141 tggaagttaa aatcgagttt acctttgacg cttccaatct tggcggtaaa cagcttgtaa
    13201 cttttgagga attatacgat ttaagcaatc cagacgaacc tatcaaagtt gcggaacaca
    13261 aggacattga ggacgacgga cagacagtta ctatcaagga agtaccagaa acacctaccc
    13321 cagaagaacc agagaagcca accacaccag atacacctac gaaaacagac agtcccaaaa
    13381 cgggcgacaa tacaaatatc cttgcctttg caattatgat gtttgtttct gctggtgggc
    13441 tggctggtac atactttttc aaacaccgta agatgaagaa atcataaggc ggtagctgta
    13501 tgagaaaaga aaaatcacgc tctccgccat agcttagata ggcagactat acctattctg
    13561 gcttgtctgc cgattacctg caagaacacg gatagtcaag ggtgcgttag catttattta
    13621 ccctttacta tctgggggct tgctggcact ttcctaaatg ccagaataca cacatcaatc
    13681 aaacttaaag taagaaacga ggtaaaataa tatggaatta aaatatatcg tgccgaatgt
    13741 ggcacagact tttggaaact tggaatttgc aggggaaaac aaagtagacc agcaacgtat
    13801 caatgggcgt tacactctcg tatcaagaag ttacaacttg tattcggata ttcagagagc
    13861 cgacgacatt atcgttgtat tgcctgcaaa agctggcgag aagcatttta agccagagca
    13921 gaaagtaaaa ctgattaacc cacgcattac cgcagagggt tacaacgtca aaggacgtgg
    13981 atttacaaac tacattttaa tggctgatga tatgttacca gcagaaagca aataaggggg
    14041 tatttgatta tgagattaac aaatggaatt gtattagata acgaaaaaac ttttggagta
    14101 ttgaaattct ctgcacttcg acatgaggtg cgtgtagaaa atgaggacgg aacgacaagt
    14161 gatgaaatta agcgtcgtac ttatgatttg aaatgtagtg tgcaggaacg catgatacaa
    14221 gtgtcaatcc ccgctgatgt tcccgtaaag gactttcctt ataatgcgga agtggagtta
    14281 atcaatcccg tagctgatac ggtggcaaat gccaacttta gagggactac ggttgattgg
    14341 tacatcaaag ctgatgatat tgtattgaag aacaaaggcg gtcaagctgg aaatccgcag
    14401 aacaacgctc cgcagggaca gcagaaaaag taagctgaca aactagtttt tcattgtagg
    14461 agatataaac aactgttata atatggatgt ggaaacagtt aatttagact tataaaggag
    14521 aaatgtgtat gctagataaa atacctaatg cagaggaaat gactgctttg gtcggacaat
    14581 cattatatag tgtatggaat gaattgtgtg ccttgattga tgaaaagtat gaaatggaat
    14641 gtctatggaa taaaggtggc aaagcatgga attatgaata taagtatcgt cgaggtggca
    14701 aaacattatg tgccttatat gctagagaaa attgtgtggg atttatgatt atcttaggta
    14761 aagatgaacg gttaaaattc gatacatata gagataatta ttcaaaagaa gttcagagaa
    14821 tttatgatga aacaaaaact taccatgacg gaaaatggct gatgtttgag ccaacagata
    14881 caactttgtt tgacgatttt gttaaacttt tgaggataaa aagaaaacca aatagaaagt
    14941 agtttattac cacaccatta gcaaatggat agtttcttat aatagcggat atatgaaaat
    15001 gctgtcatgt ggacataaag aaaattggaa gttgtggagg tagcaaaaat gaaattaaaa
    15061 aaccctatgt tggtagtaac tgatatagat aaatcagttg agttttataa aaaggttttt
    15121 ggactttatg tcattatgga ttttggggca aataaaactt taacaggtgg attagcctta
    15181 cagacaagtg aaacatacaa agagtttatc ggtacaagtg atatttcttt tggtggtaac
    15241 aattttgaag tctattttga agaagaagat tttgataaat ttgctgatag gctgaaagaa
    15301 tatgatattg aatatgtcca ccctattata gaacattcat ggggacagcg tgtagtccgc
    15361 ttttatgacc cagataaaca tattatcgaa gttggagaaa acatgaagat agtgtgcaag
    15421 cgtttcttaa atagtggaat gacaccagaa caagttgcag aacgtatgga tgtacctatg
    15481 aaatttataa atgcttgtat gcgataaatt gaaagttagg agactaatac tatgaaagga
    15541 tttgaacgca aaaatcaatt attttctctt tgcggattga attgcggact atgccctatg
    15601 cttttgggaa attactgtgg cggttgtggc aatggtaatc aatcatgcag aattgcaaaa
    15661 tgtagtcttg aacatggaca gattgaatat tgctatgaat gcaaacaata tccatgcgaa
    15721 aaatatcaac acattgatga ttatgattct ttcatcacgc ataaacggcg aaaagcagat
    15781 ttagaaagag caaaaaacat aggcattgag caatataatc ttgaacagca ggaaaaaaca
    15841 caaattcttt cttacttact ttctaactat aatgacgggc gtagaaagac tttcttttgt
    15901 gtggcagtca atttattaga actttcagaa ttacaagaag caatgaaaca aatacaatcc
    15961 aatgacgaat tgaccttact accatttaag gcacaatgct tgtatgtggt ggaggtgttt
    16021 cagaagattg ctgaaaggag aaatataaaa ctgaaacttt tcaagaaaaa gtgattttca
    16081 aatttgaaat tgtaacaaag attatttata tgacgaatgg gggaatacct ttggaaacag
    16141 tagagttaag atttcaatat actcaaagtg aatatataaa agcagaaagg caatatttaa
    16201 tttctagtaa aactatacat aaatatgata ttgctttagt cgctgtattt ttgttgctgt
    16261 cagtcgttta tatgctgttc tcctctttta gcgtatttag tattttaata tttggattga
    16321 taattgttgt aactgcattg ggaagctatt tatatatcct aatgcctata ctgaaattca
    16381 aacaaactgc aaaatatcat gaggaatata cgctggtatt ttctaaggaa aacattaaat
    16441 ttaaaacaca aagcattgac tcagaagtga agtgggacat ttactctgca ctatgggaaa
    16501 gccatgattt ttattactta attcaagcac cacgattata tacgttaatt cccaaaagaa
    16561 tttttaaaga cctaaacgaa aaacagctat ttgaagaaat tgcacaatca agagttaaaa
    16621 caacaaagca tatataatga acatacccta caatgtaaca acttcatatt catacacaca
    16681 aagcggttag tcttttaaga ttgactgctt ttttcatact taaattaaga ttggagtgat
    16741 tgatttatgc gaatgatttt gaataaagga caccgtatca gagccagaga caagaacctt
    16801 gtctaccact tttccatagg gacgcttctg tttgtatttg tggcggttct cctgctactg
    16861 aatatcaaac agctcatgcg taccgactgg gaacatttca gcttattgga caatggcttg
    16921 acgctttccc cctacaactt cataaccata ttgatagcga ctggtatttg tgcattggtc
    16981 gcttttctgt attaccgctt ctgttatgac agtttcaaaa agctcctgca ccgtcaaaag
    17041 ctggcacgaa tgatactgga aaacaagtgg tatgaagcag ataccgtaca agacagcggt
    17101 ttttttactg acctgcaaag cagatcaagg gaaaaaatcg tctggtttcc aaagatttat
    17161 tatcaaatgg aaaacggatt gcttcatatt cactgtgaaa tcacattagg aaaatatcaa
    17221 gaccagcttt tgcgattaga ggataagctg gaaagcggtt tgtactgtga gctgaccgac
    17281 aagaccttgc atgacggcta tattgagtac acgcttttat atgacatgat agcgaaccgt
    17341 atttccattg acgaagtgaa agcagaaaac ggctgtctta ggctgatgaa aaatcttgtc
    17401 tgggaatatg acgtactccc tcacgctctg atagctggtg ggactggtgg cggtaagacc
    17461 tactttctgc tgacgcttat tgaagctctg ctacatacga atgctgtcct ttacatctta
    17521 gacccaaaga acgctgacct tgcagactta ggtacagtta tgggaaatgt gtatcatacc
    17581 aaagaagata tgattgattg tgtcaatgcc ttttatgagg gcatggtaca aagaagcgaa
    17641 gaaatgaagc aacacccaga ctataaaacg ggcgaaaact acgcctatct tggtttaccg
    17701 ccctgctttc ttatctttga tgaatatgtg gcgttttttg aaatgctggg gacaaaggaa
    17761 agcgtaagtt tacttagcca gttaaagaaa atcgttatgt tgggacgaca agctggatat
    17821 ttccttattg ttgcctgcca gcgtccagac gcaaaatatt tctcggacgg tatcagagat
    17881 aatttcaatt tccgtgttgg attggggcgt atcagcgaat tgggttacgg tatgctcttt
    17941 ggttctgatg tgaaaaaaca gtttttccag aagcgtatca aggggcgtgg ctattgtgat
    18001 gtgggaacaa gtgtcataag tgagttttac acgcctttag tcccgaaagg acatgatttt
    18061 ttgcagacca taggctctct tgcattcgta aggcaggacg ggacggcgac gtgcgaagcg
    18121 aaaggcgacg gcacggacta gccgttgctg gtgtggcgtt agccacgcca gcagatataa
    18181 gcccctcatt atctaacaga ggggcacaat tacgctggaa actggttcaa aaaccttgaa
    18241 aagtccgcta ggaataagga ttttcttagt ttttagctag ttgtgataat cacttgaaaa
    18301 tatcacaaaa tcacaacaat atcacgattg ggggtatgac aactgaatga tactgatttt
    18361 ctgaacacct tgaaagcaaa acgcagtgat tatggcgtat cgcagacaag gcttgcaatc
    18421 atggctggta tcagccgtga acatctaagc cgtatcgaag ctgggaaggt agcactgacc
    18481 gaggatatga agcacaaact actggaagcg gtggaaaaat tcaatcccga cgctcccatg
    18541 ttcctgctct ttgattatgt gcgtatccga tttcccacgc tggatataca gcacgttatc
    18601 aaggacattc taaagctgaa tatagattat atgcttcacg aagatttcgg gcattacaag
    18661 tacaccgagc attactatat cggggacgtg ttcgtttaca cttcacaaga caaggaaaaa
    18721 ggtgtcctgc tggaactgaa aggaaaaggc tgtcgtcagt ttgaaagcta tctgctggca
    18781 caggaaagaa gctggtatga cttcctcatg gacgcattag ttgatggcgg tgtgatgaag
    18841 cgtatcgacc tagctatcaa cgacagaacg ggcttactgg atattccaga actgatagag
    18901 aaatgcaaga acgaggaatg tatttctaaa ttccgtagct tcaagaacta tggttctggg
    18961 gagctggtaa agcacaacga agccaacaag gacggcatgg gacatacgct ttacattggt
    19021 tcatttacca gtgaagtgta cttctgtgtc tatgaaaaga attatgaaca atcctcaaaa
    19081 ttagggatac cgattgagga agtccccatt aaaaaccgat ttgagatacg tttgaaaaat
    19141 gagcacgcct actatgccat tagagagctg ctgacccact acgacgcaga gctgacggca
    19201 ttttctatta tcaaccagta tatccgcttt gccgataagg aagccaacaa gcgaaaaagc
    19261 gactggaaaa ccaacgcccg ctggtcttgg ttcattggcg agggacgacc gcccgtgaag
    19321 ctgacgacaa agccagagcc ttacactctg caaaggacgc tgaactggtt acaaagacag
    19381 gttgcaccga ccttgaaaat gctgaaacag atagacgctg gcaatcagat ggactatctc
    19441 aagaccattg aacagcaggc aaagctgaca gataagcaca agcagataat cagacagcag
    19501 acggcttcgg tggacgaact gatagaaaaa gaaaaggagt gattgatgat gtgggaattg
    19561 attaaggact tactgctggt atcacttggc atgggtatcg gcgtggttct gatgtgtatt
    19621 atgagtgtcg gaaaggaagc tgaccgacac atgattgaat taaaagaaag cgaggaaaaa
    19681 taaatgaatt tcgggcaaaa tttatataca tggtttttaa gcaacgccca gagcttagtg
    19741 cttatggcga ttgtggttat tggtatctac ttagggttta aacgtgagtt ttcaaaactt
    19801 atcggcttct tggtggttgc cttagttgct gttggattgg tattcaatgc aggcggtgtc
    19861 aaagacgtac tcttagaact attcaataag attatcggcg catagccgta ctttttcatt
    19921 atggtttgcg gatacgcaaa catacctttc ccactttggg agtgggctaa ggggaaaggg
    19981 ggtaacggaa tggtacacag ataaaaatac agcataatga atttcattgc ttggctcaaa
    20041 catagttgat ataataaaga tatgaaaata ataaacgaaa gtttttttga taaggagtga
    20101 tattgtgaaa ttgtgtattg ttttatctcc atataaggct taatggaata gacttgtatg
    20161 gagtgaaatg ttatccagag agccttacat tttatcaaat ttcaaaatgt aaaggagaat
    20221 gggcatggaa tataaatata caaaaattga tttgaataaa tggaatagag gtaaattgtt
    20281 tcaaagctac attgataata tgcgtattgt tatgagctta accgtagata ttgatgtaac
    20341 aaagtttatt gaattttcaa agaagcatga tttaaaattt tatccagcta tgatatgggc
    20401 tgtttcaaag gttgttaatg aacatgatga gttcaaatat agttgggata ataatgggaa
    20461 tttaattaag tgggattata tttctccgtc ctatactgaa tttcataaag aagatgaaaa
    20521 tttcacaaag atagttacag atttttctga tagcatattt gaatttcata aacgttttat
    20581 attagatagg gagaagtata aaacagaacg tgcatttgtg gaaaatcaat ctttgaactt
    20641 ttttgatgta tcttgtttac cgtgggtaaa atataatcat tttgatgttc atgtttttga
    20701 cgagggaaaa tttcttgcac cagttattac ttggggcaaa tatgaattag agcaagaaag
    20761 atatataatg cctcttacaa tgaatataca tcatgccgta gcagatggat tccatttatc
    20821 cagatttttt aatgaagtac aagaattgat aaactcaatt taacagctat atacaaagca
    20881 gttagtctat acgattgact gctttttttc atacctaaat ttaagattgg agtgattgct
    20941 tgaaagatat attttcggat atgcaggcaa gtatcggctg tacctacatt tcagatttac
    21001 cacatcataa acgtatggtg tggcaggaaa tgaagcgact gaaccttgcc gattatccgc
    21061 agaaacaatt agaagatttt gcaggctatg tctttggtgt cccttacact agcatagctg
    21121 aagcatgaaa gaaaggacgt gaaaacctat gaatgaaatg cgtgtctgta tcgaaagccg
    21181 actacacact tctgaagaaa tccgttccgc atggttcaca cttcccattg acgaggaaga
    21241 actggaagaa cagataggcg ttcctatcga cagtgaagat taccatatcg ttgaaaagga
    21301 actgcccttt gctgatgaag tcggcgaaga tgcgaccatt gaaaaactga atgaccttta
    21361 ccataccttt gaaagtctgc ctgccgattt acaagaggac tacggggagc tgatgtgtta
    21421 ctttaccagc cttgacgaac tgcaccagca ccgccatgat gtgatacatt attcatggtg
    21481 taaaaacatg gctgatgtgg caaggcattt gttggacaat gaccctgctt tttctgccat
    21541 cgaggaacgc ctgacccgtt actttgatta tgaagcctac ggacagtatc ttgatgataa
    21601 cggagaattt atagaaacag accacggtat ctatgaactg ccgtagaagt gaggtgtttc
    21661 tatggtagag gatatgcgtg tatatatcgc taatcttggg aaatacaatg aggggtatct
    21721 ggtcggcgac tggttctctt ttccgattga cgaagaagat gttgccgagc gtatcggctt
    21781 gaatgaacgg tacgaagaat acgccgtaca tgatacggaa aactttccca ttgagattgg
    21841 cgaatatatc tcgattgaag agctcaatga aatgtatgaa atgatatgtg agcttcccga
    21901 ctacatcacg gacagtttgg acgaatttgt atcaaattat ggaagtttgg aggaactgta
    21961 cgaccatagg gacgatatga ttttatacgc tggctgtgag gatatgaccg acgttgccta
    22021 ttacttcatt gatgaattgc aggcacttgg cgatattccc cctagtttgc agaactatat
    22081 tgactatgaa gcatacggca gggatttatc ctttgaggga acttttattg aaacaagata
    22141 cggtatgtgt gagataccgt ggtaagaagc tggaagatca gcgacaagca ttgttgcttc
    22201 tgacagcttt ttctattggg gacagtctga aaaacggctg tccttttcta ttatacgaaa
    22261 ggagttgaaa caaaaacgtg aaaaagatta aaagctacac gggtatctgg aacgtggaaa
    22321 aagtcttgta tgcaatcaat gactttaacc tgccctttcc tgttaccttt acacagatta
    22381 catggtttgt gataaccgag tttatcatca ttctgtttgg ggacttaccg cccttatcca
    22441 tgattgaggg ggcattttta aaatacttcg gtattcccgt agctcttaca tggttcatgt
    22501 cgcaaaagac ctttgacggc aagaagccgt acagcttttt gaaatcacag ataacctttg
    22561 ccctacgacc aaagattact tatgcaggaa aagccgtgaa actggataag caggtactac
    22621 atgaagctat tacggcagta aggagtgtga gctatgttcc cgattaaata tatcgacaac
    22681 aaccttgtct ggaacaagga caatgaggtg ttcgcctact atgagctgat accttacaat
    22741 tacagttttc tatccgctga acagaaattc attgtgcatg acagcttccg acagctaatc
    22801 gcacaatccc gtgagggcaa gatacacgct ttgcagattg ccacagaaag ctcaagccga
    22861 agcatacaag aacagtcaaa gaagctggtt acgggacgac taaaagacgt tgcctatcag
    22921 aagatagacg aacagaccga agcgttagta tctatgattg gggacaatca agtggactac
    22981 cgcttttttc ttggcttcaa gctcatggta acagaccaga gcatgaacct aaagagcgtg
    23041 aagcaatcgg cgtttctcac attcaaggag tttatccatg aagtgaacca cacgctgatg
    23101 aatgactttg tttccatgcc aaatgatgaa atcaaccgct acatgaaaat ggaaaagctg
    23161 ttggaaaata aaataagccg acgcttcaaa gtaagacgct tagataaaaa tgacttcggc
    23221 tatctgattg aacacatcta cggcagggac ggtgtcgcct atgaagatta tgagtatagc
    23281 ttaccgaaga agaaactgaa aaaggaaacg ctgataacgc attatgactt gatacgtccg
    23341 acccgttgcc ttgtggaaga aagccagcga catttacgct tggaacatga ggacagcgaa
    23401 agctacgttt cctactttac tgtcaatgcg attgtcggcg agcttgattt tccgtccagt
    23461 gaaatcttct atttccagca acagcagttt acttttcccg ttgataccag catgaatgta
    23521 gaaatcgtgg aaaaccgaaa agcgttatcg acggttcgca acaagaaaaa agagctgaaa
    23581 gatttagaca atcatgcata ccagtctggc agtgaaacca gctcaaatgt ggtagacgca
    23641 ttagacagtg tggacgagct ggaaacggat ttagaccagt ccagagaaag tatgtataag
    23701 ctctcttatg ttatccgtgt atctgctcca gaccttgacg agttgaaaag acgctgtgat
    23761 gaagtcaaag acttctacga cgatttgaac gtcaagctgg tacgccctgc tggcgatatg
    23821 ctggggcttc actctgaatt tcttcctgcc agcaagcgat acatcaacga ttatgtgcag
    23881 tatgtcaaaa gtgattttct agctgggctg ggctttggtg cgacacagca gttaggggaa
    23941 aacacgggta tctatatcgg ctattctgtc gatacgggac gcaatgttta tctacaaccg
    24001 tcccttgcca gtcaaggcgt aaaagatacg gttactaatg ctcttgcttc tgcctttgta
    24061 gaaagtcttg gcggtggaaa atcattctgt aacaacctgc ttgtctacta ctctgtgctg
    24121 tttggcggtc aagcggtaat cttagaccca aaatccgagc gtggcaactg gaaagaaacg
    24181 ctcccagaaa tcgcccatga aatcaatatc gtgaacctta ccagcgacag agaaaatgca
    24241 gggcttcttg acccgttcgt gattatgcag aatgtgaaag acgctgaaag tctggcaatc
    24301 gacatactca cattccttac gggcatttct tcaagggacg gcgagaagtt ccccgtactt
    24361 agaaaggcag tgcgtgccgt tacccagagc gaccagcgag gattgctcca tgtgattgag
    24421 gaattaagaa aagaagatac acctatctct tgcaatattg ccgaccatat cgacagcttc
    24481 acggactacg actttgcaca tctgctgttc tcggacggta cggtgcaaaa tgcaatcagc
    24541 ttagacaacc agctcaatat cattcaagta gccgacttag tgctacccga taaggacacg
    24601 acctttgaat tactgtccgt ttccatgctg attgtgatta gtacctttgc gttagatttt
    24661 atccactcgg atagaagtgt tttcaagatt gtagaccttg acgaagcatg ggcgttctta
    24721 aatgtggcac aaggcgaaac gctctctaac aagctggttc gtgctggacg tgccatgaac
    24781 gcaggcgttt attttgttac acagtcctct ggcgacgtgt caaaggaaag tctgaaaaac
    24841 aatatcggtt tgaaatttgc ctttagaagc accgatatta acgaaataaa acagaccctt
    24901 gaatttttca gcattgacaa agaggacgag aacaaccaga agcgattgcg tgatttggaa
    24961 aacgggcaat gcttgcttca agatttatac ggacgtgtgg gcgtggtgca gatacaccca
    25021 gtctttgaag aattactgca cgcctttgat accagaccgc cgatacagcg aaatgaggtg
    25081 gagtgatgaa agaaaggata aaaagtgtgt tcacaagaaa aaagatattc cgctttctca
    25141 aaatggctct gtttgtcgtg gcactttccc ttatcctgct ttcacttttg ggaacggtgg
    25201 cacacgcaac gggacttgtg gacgacacta tcaacaccga gaacctttat tccaaatatc
    25261 cgctgtcgaa ttatcagctt gatttctatg cagataataa ctgggcgtgg ttgccgtgga
    25321 actggctgga cgggattgga aaaagtgtgc aatatgggct ttactgtatt accaactttg
    25381 tctggacgat aagactatat ctaagcaatg caacgggcta tgtagtgcag gaagcctaca
    25441 agctggactt tatcaacgat atggcagaca gtatcggaag aagcatacag acccttgcag
    25501 gcgtaactca aaatggtttt tctacttcgg gcttttatgt gggatttctt ctgctgatta
    25561 tcttagtcgt aggtatctat gttgcctata cgggactgat aaaacgggaa accagtaagg
    25621 cactacacgc cgttatcaac tttgttgtgg tgttcgtatt atcggcttct tttatcgcct
    25681 atgctcctga ctacatcaag aaagtgaatg acttttcatc agacatcagt accgcttcac
    25741 tggacttggg aacaaaaatc atgcttcccg acagcgacag caagggcaag gacagcgtgg
    25801 acttgataag ggatagccta ttttccatac aagtacaaca gccgtggcta ctgcttcaat
    25861 ttggtaattc gggtatagag gaaatcgggg cagaccgtgt cgaagctctc gtatcggcaa
    25921 gcccagagga cgaggacggc aagaccagag aggaagtcgt gaaaacagaa atcgaggaca
    25981 acgacaacaa caatctgacg atactgcagg tggtaaaccg tttaggtatg gtgttcttcc
    26041 tcttgttctt caacttgggt atcacgatat ttgtattctt gcttacgggt atgatgttgt
    26101 tcagccagat actttttatc attttcgcta tgtttctgcc tatcagcttt ctattaagta
    26161 tgataccgag ctatgagagc atggcaaaac aagccatagt ccgtgtgttt aatacgatta
    26221 tgacaagggc aggtattaca ctgattgtca cggtagcctt tagtatttcc agtatgttct
    26281 acaacatatc gacggactac ccgttcttta tggtggcgtt cttgcagata gtgtattttg
    26341 caggtatcta catgaagctg ggcgatttaa tgagcatgtt ctctttaaac gctggcgaca
    26401 gtcaaagcat gggacggcga atattccgca gaccgtatct gttcatgcga catagggcta
    26461 gacgtatgga acataggctt gcaagagctg ttagtgctgg tggtgtcgct ggtgcggtgg
    26521 ctggtggtgt cgttgccagt tcaaaatcta cacgccagaa tggaacaaag aacaatcgtg
    26581 gaaatacaag ctccagcatg gggcagaaag caggttcagc cgtgggagct gtccttgata
    26641 caaagaataa ggtcaaggac aaggcaactg ctgtcaaaga aaatatcaaa gatatgccga
    26701 cacagactgc ctatgcggtt cactctgcta aggaaaaggc gaagtccagt gtgtctgatt
    26761 tcaagcgtgg cgtggtgcag gaaaaggaaa accgccagtc tggacgctta aagaagcaac
    26821 agctacatag acagaatatc gctgacaagc gtatggaact tcaaaaggca caagaatcaa
    26881 ggcaggcagg taagaaaacg gacggatcag cgacaagcgg agctacccgt ccccatgaaa
    26941 gaccagctac cacttctaag gttaatgtag aaaaacagca ggaagtcaaa cgccctgcca
    27001 tagcttcaac cgtgaagaat gatgaaccta tcaagaaaaa tgttgtcaag gaacgtccac
    27061 tatcttctgg cgatacatca gcaaagaccg tcaatcaaga aaccacaaga cagaattaca
    27121 agactgaccg tgttacgaaa acaaccgttc agaagcatac acagcagaat accgagaaga
    27181 acaaacgaac cgtattcaag aaaggacaaa agaagaaatg aaactaagac atatcgctat
    27241 tgttggcagt ctgttttcta tcctcttttc tctcgtatta ttctttgggg ttctgattag
    27301 tgccgaggac gacgacgggg gcaatggtta ttcctctaat tatgtaggta tgaacctatc
    27361 tgccgaagtc ttgaaacatc agcctatggt agaaaagtat gccaaagaaa acggtatttc
    27421 cgagtatgtc aatgtgttgc tggtaatcat acaagtggaa agtggcggta cagccgagga
    27481 cgttatgcaa agctctgaaa gtctgggact accacccaac tcactagata cggaaagctc
    27541 aatcaagcag gggtgtaaat attttgcgtc cctgctttct tcctgcaaaa atcaaggtat
    27601 cagtgatata aatgttgtga tacagtccta taactatggc ggtggctatg ttggctatgt
    27661 ggcaggaaac ggaaaaaagc atatgttcaa ccttgcggaa aacttcgcaa gggataagtc
    27721 gggcggtcaa aaagtaacct atacgaaccc tatcgccgtg gcaaaaaatg gtggctggcg
    27781 ttatggctat ggaaatatgt tctatgtgga gcttgtgaac cagtaccttg ttgtcaatca
    27841 agtatcgggc gagctggcac aaaaggttat gaatgaagcc ttaaaatatc aaggttggaa
    27901 gtatgtatat ggtggcagta acccgaacac ttcttttgat tgttcgggac tggtacagtg
    27961 gtgctacaac aaagctggcg tatctttacc gagaaccgca caaaatcagt atgacgcaac
    28021 ccagcatatc ccactatcgc aggcaaaggc aggcgatttg gtctttttcc acagtaccta
    28081 caatgcagga agctatgtaa cccacgtcgg tatctatgta ggaaacactc agatgtatca
    28141 tgcaggcgac cctatcggat atgccgattt aaacaatgct tattggcaac agtacttgat
    28201 ttgtgctgga agaataaaac agtagaaagg attggaaacc atgtttaaaa agaaagaaaa
    28261 accagtaaaa gaaacgaaag tccgcactat gaaagtaggc acacataaga aatctgtgat
    28321 tgctctgtgg gtggtgctta tcgccagcgt atcatttggc gtgtataaga actttaccgc
    28381 cattgatatg cacacggtac atgaaacgga aaccattcag ttaagactaa atgacaccaa
    28441 cggagttgaa aattttgtga agaacttctg taagtcctac tacacatggg acaacagcaa
    28501 ggaagctatc gaagcaagga cgcaggcaat cagcaactat ctgacaaagg agttgcagga
    28561 cttgaacctt gataccatta ggacggatat accgacaagc tccactgtta caaatgtact
    28621 gatttgggat ataaaacagt ctggaacgaa cgattttatc gctacctacg aagtagatca
    28681 gcagataaaa gagggagaac agacaagcaa tgtcaaagca acctataccg tcaaagtcca
    28741 tgtggataaa gacggcgata tggttatcgt acagaaccca accattgcac cagcgattga
    28801 aaaatcagac tatgagccaa aggcacaaga agccgacgca agcgtggacg cagataccgt
    28861 caatgacgct accgcatttt tggaaacttt ctttaagctg tatccgacag caacagaaaa
    28921 agaacttgcc tactatgtgg ctggcaatgt gcttgaacct atcggcaggg actacctcta
    28981 ttccgaatta gtcaatccta tgtttacgga tgacagggat aatgtgaaag tcagggttgc
    29041 ggtgaaattc attgataatc agacaaaggc aatgcaggta tcacagtttg agcttgtgtt
    29101 acataaggat agcaactgga aaattattat gtaagatatt ttgttggcag gctctatttt
    29161 cggatatagt ctgccttttc tatagcaaat gtgctgcact aacaagcgat ttcattgtat
    29221 tttaagcaat taaatctttc ttgacaattt tatcttaaat catatattta tggatattga
    29281 ctttctgctt gcaataagtt ataatatgac tataaaacta aattagtcat ataggaggcg
    29341 tgtggtatgt ataaagaagt tggtatttct tcaaataatg ttccgataac actttctatt
    29401 tggtatacag aaagaaatag tcctaccatt gtttttttgc cgggtactat gtcacaccct
    29461 ctcatgtatg agaattttct atgtggtttg agtgaaagag gatttaatat aataggcatt
    29521 cactatcttt cacacggaaa aagccctaaa atcaaaaaaa attatacaat ggaagatttg
    29581 ctgcataatg tatatgacgc aacgacttac ggaatagaaa attttggtga aaacatagct
    29641 gtcatgggtt ccagtcaagg tgggatttta gctgcaatgg cggcgggtag ggacacaagg
    29701 ttaaaagccg tttttccaca taatatcatg ctgactacat taaaagaaac tatgagctta
    29761 acaaaatacc ccgcatggtc acaccgtttt atggggctga tacagttggg ctttcgtgta
    29821 ggtggaaaat tgctgcctaa ctataaggtt ccaggagacg cttatttaga ctatgacaga
    29881 gtattttata gtgaacaggc aaaacaagat tgcttaaatg accctatgct tttaccctat
    29941 tatcctttaa gatttgtcgc cagcttattt aatgctgact tatcgtgctt ggagaatggg
    30001 gctatacaat gtcctgttat tttaattact gccaaaggcg atccgctttt tagatttgat
    30061 tataccaaca aagtatttga tttaattcat gtaccgaaaa aggaattact tactttagag
    30121 cttaatcgtc acatgatttt taatgaggac acggacacta caataaacgc attggaagct
    30181 accttgcata aatatcttgg atagtatatt gattatataa tcagtaaatg ttatactttt
    30241 catgacgata aaaacatttt agtcataagg gggttatgag tttgccacga tttacggaac
    30301 aagaaaaaga aatcataaac agtaaacttt tgatagaggg agaaaagctg ttttcattat
    30361 acggcttaaa aaaagtaaca gttgatgatt tagcagctgc tgtaaacata tcaaaaggtt
    30421 cattctatgc tttttatccc agtaaagaac atctatatgt ggaaatcaat tttcgcttac
    30481 aaaaagagtt atttacaagt atagaaacaa ctgttaaaaa gaaaaagtat aagaaccata
    30541 gagatttagc aaaggacgtt attatgctaa gtttaacggg ggtgattact tcgcctattc
    30601 tttcacagat tgatttatct ttaatggact atctgcaaag aaaaatatcg tcagatattt
    30661 ttgaaaatca tatgtacagt gatattcgta tattggagat actggaaggt ttaggggttg
    30721 tatttttggt tccgcatacg gttatcataa aatccctgta ttctgtttta tcctgtttgg
    30781 aacaattcaa agaggacgaa gaattgaata tggtacaaaa tcttttagtg aatggtatta
    30841 ttcagcaagt agtagcagag taataatttt gtttggttat ttgacaggca agtggccaaa
    30901 caaatttttt acagtttata tgactatata tctataaacg tcataaagtt aaaggaggaa
    30961 agaataatga ttgatgtaaa aaaactgtat tttagttata ctgacaaacc gtttgttgag
    31021 aatgtaagtt tccatgttgg gagaggagaa attttcggtt ttttaggacc ctctggtgct
    31081 ggaaaatcaa ctattcaaaa aattttaact gggcttaata caagttataa aggcagcgta
    31141 aaagttgcag gtacagaaat aagggagcat acaaaccggt tttatgaaaa tattggagta
    31201 gactttgaat tttcaacctg ctacgagaaa ttcacagcac gacaaaatct cgcctatttt
    31261 gcttctctat atgacaagca acctcggtct attgatgaat tattgcatat ggtaggctta
    31321 gaaaatgatg gggacaaaaa agttgctgac ttttccaaag gtatgcgttc tcgcttgaat
    31381 ttcattaaag cgttagtaca tgaccctgat atattattcc ttgatgaacc tacaagcggt
    31441 cttgacccta caaataatcg tttaatgaaa gatattattc ttgcggaaaa gaaacgcgga
    31501 aaaacgatta tcattactac tcacaatatg tatgacgcaa cagagctatg tgaccaagta
    31561 gcctttattg ttgccggaaa agtcagtgca ttggatagtc cacataattt gattatgtca
    31621 aggggagctg ccaaaataca ctatacctac tatgataaag gcgagaaaac cggagaatgt
    31681 ctgcttgata gaaccacaga agataagttg ttaaaaagtt taatttccga aaaccgtctg
    31741 ctttctattc atagtagtga gcctacatta aatgatattt ttgttgatat tacagggagg
    31801 acacttcaat gagattggga cgtttaattt gtggtgatat acatttccaa tggaaatatg
    31861 gtttctattt tatctacttt atattaacag ttttatatgt gtgtgggatt gccgctttga
    31921 cgggacattg gaaaacggat attgcttcta tcatgatata ctctgacccg gcagctatgg
    31981 gattgttttt tatgggggcg attgtccttt tagaaaaaag ccaaaaggta ttaaatgcta
    32041 tggtagtatc gcctgtaaaa gtatcggagt atatactttc aaaaaccatc gcattgattg
    32101 ctatttctac aatcattgcg gtgatattgg gatttgtttc cggcagcaat catttacttg
    32161 gtatcgcagt tggtacagct ttgacatcag caatctttac tatgcttggc attatcgcgg
    32221 ctacaaaaat ttcaaacttg aaccaatttc tcattgtgat tatgccgata gaaattgttt
    32281 gctttgttcc acctatcgtt ggtctgtttg taaagttacc agatatattc cgcttttttc
    32341 cgttcacagc ttgcatgaac ctcataactg gaaaaagcat tttactatcg tttgatatgg
    32401 tacttgtaat tgctacgttg attatcctct acatagttgc gcgacataca gttaagcata
    32461 tgtggagaag tttgggaggt gtaaagctat gaaaaggata ttatccagta ccatacaggt
    32521 atttaagcaa atcaagagcg accctatgat gtttgcagct tgttttacgc cttttgtcat
    32581 gggagctttg attaaattcg gtattccctt tcttgatgga ataacagatt tttctttgca
    32641 aacatactat ccgatctttg atttattact ttctatcatg gctcctgtat tgctctgctt
    32701 tgcatttgca atgattacat tagaggaaat cgacgataaa gtgtcacggt acttttcgat
    32761 tacccctctt ggtaagtcgg ggtatctctt tacaaggttg ggagtaccct caattatttc
    32821 ggcggtcatc gcttttattg tactgttgct tttttcatta gaaaagttgt ccattggaat
    32881 gacaatatgt ttggcgcttc tcggctcagt acaggcaatt attgtttcgc ttatgattat
    32941 aacattatca agcaataaat tagagggtat ggcagttaca aaactagctg cacttacatt
    33001 actcggaata ccagctccct tttttattga tagttactac cagttcgcgg ttggcttcct
    33061 tccatcattt tgggtagcga aagccatgca gaatgaagcg gttctttatt tctccatagg
    33121 attgatggta gctttggtct ggtactactt ccttataaaa cgtctgttta ggaagctggc
    33181 aggataagaa ggcatattat ggaaaaaggt agacttctgc aaaacgaaaa tataaataag
    33241 gctattcagg tttccaaaat cacactaatc atcaacctgg gtttatcgct acttaaattt
    33301 gctgccggat atataggaaa gtcaagtgcc atgctgtcgg acgctgtaca ctcggcttct
    33361 gatgtattaa gcacaatcgt tgtgatggtt ggaattaaaa tatcagaaaa acagcctgat
    33421 aaagaacatc cttatgggca tgaacgtatg gaatgtgtgg cttccattat tctctctgtt
    33481 gcattggcta taacgggtgc tggaatagga tattcaggta tcaagaaaat cttttcagga
    33541 cagtacaata cgctttctgt ctctagtgga atcgcactta cggcggcagt cttatccatt
    33601 gttataaaag agtggatgta ctggtataca agaagtgctg caaagcatac aaattctgac
    33661 gcacttatgg ctgacgcatg gcatcaccgc tctgatgcac tctcgtcggt tggttcctta
    33721 attggcattt taggagcacg tttaggctat gctattctcg accctattgc aagcattgtg
    33781 atttgcggtt gtatcttaaa agctgctttg gacattttca aagaaagcat taacaaaatg
    33841 gtagaccata gttgtgacaa tgctactgaa acgaagatta gggaagttgt gcttcagcag
    33901 caaggggttg atggtattga tgaattaaaa acccgcatgt ttggagcgaa aatgtatgta
    33961 gatattgaaa tattagctga tggtaattta gctctttatg acgctcatag aattgctgag
    34021 ggagtccatc aaacgattga aaacaacttt ccacaatgta aacattgtat ggtacatgtt
    34081 aatacgaatg aacttcccta ttctccatag gacagggaag atgatttaag actattgaaa
    34141 gggaaaggag gggaagtagg ttatggaaca atactggtgg attatcattg ttatggtttt
    34201 gattgtcgtt ggcttactta aaattgtaat tatgaagaag tataaccaaa ggagaaattc
    34261 taacaatcgt tcaaaaaata gcgaggatga caagatgtat taagagcaat tatccgatgg
    34321 tatgagatat atacctatga aatttgaacg ataaatccca gtttgtaaaa gtcaataaat
    34381 ccaaagataa atttgtattt gaaaggagtt ttgaagaatg aaaaaatatg agtatgtaga
    34441 aattaattat aatgcgaaag gagttgtatt cctttgtact gataaacaca aagaaattat
    34501 tgacagttat gcaaaaacgg ggtatcacta cgcagggttt gtacccacag aaattgacgc
    34561 aaaaggctgt atgcgaaaga tagatttaat ttttgaaaaa gaagattaac ttattttagg
    34621 gagttggttc taatgatagg acttaaaaga cgagatatga attatattat gaaagttggt
    34681 gctaaagctc aaggtgtttc tgtatctgaa tttagggcta tgctacaaga taccatagac
    34741 aaaactatgg aaagtacaga cccagacgtg caggcaaatt ttaaacgata ctttggaaat
    34801 aagcggccaa caccagagga atatatttat acgattacca aaaagacaaa ggttaaggtt
    34861 taaatgttgt aaagggggct tgccgtatga aacgctttaa atacaattca gtatattcaa
    34921 gcccaattta acagcatact atttacaatt aaatacgcta taccagtagc tcgtacaaaa
    34981 tttatatttt gtgcgggctt ttttaatacc cgaaaagttt tttaattatt ttcatttttt
    35041 tggaagcaaa tcacaccttg ctgtacagat atggtgcgga aagagggata aacacttttc
    35101 aattcatagc tggaaaggag gtgagattat gaaaccgtct gacttccaaa aaacaattca
    35161 gtgtcagttt gaatgtaagc tgaaaaaagt tgtcaaaggt attatctgga actaccgcaa
    35221 agaattaaga cgaagaaaaa acaaggaaat atcttttagc gaattaccag acattacggt
    35281 agaaagtctg gcaacatggg acgactacga aacaaactac acaattttta atgtgtgtgg
    35341 catagatatt aaagtccttg atgatgaact tgcagaagct ctcaaacagc tacccgaaaa
    35401 aaacgtaaca atgtattgat gtattacttc ttggaaatga gtgatacaga gattgcagat
    35461 attatgaatt ataccagaga ggctgtgttc aaacacagac accgtgcact agaaactatg
    35521 aaagagatac taaatgaaaa gacaagacag taaaggacgc aatttgcgtc ttggagagag
    35581 ccagagaaaa gacggaagat acgtttataa atataccgat atattcggga agccacagtt
    35641 tgtatattct tggaagcttg tacccacaga caagacacct gctggaaaac gtgatgatat
    35701 atcgttgagg gaaaaagaaa agcagataaa aaaagacctt gacgacggta tcgacacggt
    35761 gggcggtaaa atgacagtct gccagcttta cgaaaaaaag aacaatcaaa gaaagaacat
    35821 caagagaaac acggaattag gacgacagta tcttgtgaac gcactggaaa atgacccatt
    35881 gggtatgagg gcgattgata cggtcaagca gtccgacgct aaagaatggg ctatcagaat
    35941 gaatggcaaa ggttattctt acaagacgat agataactat aagcgttccc taaaggcttc
    36001 attctatatg gcgatacaag acgactgtat cagaaaaaat ccttttaatt ccaagttgag
    36061 tgatgttctg gaagatgata cggaagcaaa ggttatcctg acaccagagc aggaagaaaa
    36121 actccttgcc tttatggaaa cagataatgt ttacagcaag tatcgtgatg aagttattct
    36181 tctgctggaa acgggacttc gtatctctga attatgcgga ctgacaacac acattgatat
    36241 gcttaaccgt gtgattaaca tagaccacca gctattgcgt gatacagagg tgggctacta
    36301 tatttccaaa gctaagacaa aaaacggtaa gcgagagctt ccattaacag aaagagcata
    36361 tcaagcactc aagcgaattt taaagaaccg aggaaaagca caaccgcttg ttgttggtgg
    36421 ttatagcaat ttcttattct tgaaccgtga gggattgcca aaggtagcag gaaattatga
    36481 aagtatggtg cgtgggttga ttaagaagta caacaagacc cataaggaca agttaccaaa
    36541 catcacgccc cattcattca gacatactta ctgtacgaac atggcaaata agggaatgaa
    36601 cccaaacacc ctgcaataca tcatgggaca cgcaaacata accatgacat tagggtatta
    36661 tgctcacggt actttccagt ctgcaaaggc agagttagaa aggctggctt cttaatatcg
    36721 aaaatgtatc ttactactta tttactactt ttggttgcat tttcatgctg gtaaatgcca
    36781 gcttatgcaa ggtatcttcc aaaaagaaaa tgcccataca gcccgaaaat gcgggatata
    36841 tcggcatcta ccaacttatg aaaagataat cagaaattta gtaagttttt ataataaaaa
    36901 aataagaggc tgtccaaaaa tagagataag ccttttttca taatcggaat tataaaaata
    36961 cacttgattt catatgaaaa agagtgtctc ataaactaaa aagtttactt tgagacacta
    37021 ccttttttat tcttatttat actttgtaga catattataa tatgcgtttc actggtatct
    37081 gaacttctgt caaccagtct tcttcactct ctttattcca tataccatct atataagatt
    37141 ctctaggatt tccaaccact ttatatttat tgtcctctat ccatttaaat gcatctgcat
    37201 aagtttttcc taatgttgaa tatggaccat tgtgtaatat acaaactgca ttttcaactg
    37261 catctaattc tttaaatttt aatatatccg tatcttcctt taaatcattt atagcttgac
    37321 acatttcaac atccatatca gtctctctat attcaccatc atgatatata ttaaaacaat
    37381 aatcaggaac tgcacatgta caccctgttt tcatcatttc ttcagccatt acatttggaa
    37441 atagattaaa aagttcatca tatcccttta acacttttct catagaagca actttaactt
    37501 ttggtaaaga cttaataatt acatcattca tattgacttc tcctttcaaa ttcataaggt
    37561 aattacgtag tttaattatt ttatattctt cacttttaat tacatccaac atttcttttt
    37621 ctttattcat taaaatattt tcaacttctt caggattttc tacagctttt tttatctcag
    37681 ctaatgataa acccatttgt tttaaagtta ttatctgatg taatttaggt aattgctctg
    37741 ttgtataata cctatatttt gtaaattcat ctacatattc tggttttaac aaccctatat
    37801 cttcataata ccttaatgtc tttgtagtta ttttatttat tttagaaaac attcctatag
    37861 aaaacatctc atcacctgcg cattagattt gattaattta agtttaaact ttccccttag
    37921 gttaaagtca atacaaaata aattataatt gtattaattc ttcaattcta tctccactca
    37981 ataccaaaat atcccctgtt tttataatgg ttgaaccttt aggtattaaa acctgttcct
    38041 ttctttttat cataactatt aatatatcgt ctggtatatt agaatctatt atactcttat
    38101 tagcccattt attaccctca tcaacattta cttcgataag tttagagcta atttccccat
    38161 cataatcatt aaatgttttt aatacaggtg tattatcatc aactaaatcc aactttctgg
    38221 ctacagttgg tatcaatgaa ccttgtattc ctactgaaag caacgctata aaaaatatta
    38281 tatggaatat gtcattctct atggctactc cttgagtaac agcataaatt gcaaaaacta
    38341 ctgaagccgc acctctaaga cctacccacg atatgaataa tttttcttta tttgagaatt
    38401 taaattttgc tagaactata aatgttgcta atggtcttgc aataactatc atgaatatag
    38461 atatggccat tgctatacca aatacatttg gtaacttaga agggaaagat aacaatccta
    38521 acataaagaa taacattatt tgcataatcc atgaaattcc atcaaagaaa tgaaccaaac
    38581 tttttttaag aggtatccta ctatttccta taataatacc tgaaatgtaa acacttaaat
    38641 atccatttcc tccaagatac tcgctaaatg cataagaaag tattgcaata gctgttataa
    38701 atatagtata aaatccttct atctcgaagt ttgaatgcct taaaatataa acagataatt
    38761 ttgctagtat aaatcctata gctaacccaa aagctatttg ttttattacc attggtgcaa
    38821 taagttctac cccagaatta ttcatcaatg tcaaaagaat tagtgtcaac atatacgcta
    38881 ttgggtcatt acttccactt tcaacttcta ataatgatgc aagtgaaccc cttaagttta
    38941 gtttttgtga acgcaacaca gcaaacacag atgcagcatc tgttgaagct gcaattgccc
    39001 caattaataa accttcaagt aaactagttc ctattaccat gtggcaaaac atacctgtaa
    39061 ccccagacgt aattattaca cccagtgaag acattaatat agaaggtata gcaacaggtt
    39121 tagccatatt ccagtttgtt ccaaaccctc caaaaaacat tataaatatt agtccaatag
    39181 aacataattg ttttgttaat tcatagttct gaacctccca tgactaaagt cacagagttc
    39241 ctgcttcata gaattttgca tactaaaata tgtcttacta attctccaca ggctatcccc
//
LOCUS       DAMJVX010000018        37988 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMJVX010000018 DAMJVX010000000
VERSION     DAMJVX010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37988
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.019"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(217..960)
                     /locus_tag="PC407_000893"
     CDS             complement(217..960)
                     /locus_tag="PC407_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2496354.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            1126..3693
                     /locus_tag="PC407_000894"
     CDS             1126..3693
                     /locus_tag="PC407_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2496355.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNNSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAVDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            3923..4327
                     /locus_tag="PC407_000895"
     CDS             3923..4327
                     /locus_tag="PC407_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2496356.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            4351..4848
                     /locus_tag="PC407_000896"
     CDS             4351..4848
                     /locus_tag="PC407_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2496357.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            4851..5252
                     /locus_tag="PC407_000897"
     CDS             4851..5252
                     /locus_tag="PC407_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDF2496358.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            5270..5758
                     /locus_tag="PC407_000898"
     CDS             5270..5758
                     /locus_tag="PC407_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2496359.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            5785..6540
                     /locus_tag="PC407_000899"
     CDS             5785..6540
                     /locus_tag="PC407_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2496360.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            6540..7403
                     /locus_tag="PC407_000900"
     CDS             6540..7403
                     /locus_tag="PC407_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2496361.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKKKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            7450..7929
                     /locus_tag="PC407_000901"
     CDS             7450..7929
                     /locus_tag="PC407_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HDF2496362.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            7994..9301
                     /locus_tag="PC407_000902"
     CDS             7994..9301
                     /locus_tag="PC407_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2496363.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETDMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            9612..9752
                     /locus_tag="PC407_000903"
     CDS             9612..9752
                     /locus_tag="PC407_000903"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HDF2496364.1"
                     /translation="MNGEGCILSNKITKECYKTGYMIREKSIDTYPDSGRGFMTWDEN
                     GE"
     gene            10264..11358
                     /locus_tag="PC407_000904"
     CDS             10264..11358
                     /locus_tag="PC407_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2496365.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQTQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSLLSENKEYKFLIEKLSLLEE"
     gene            11363..11563
                     /locus_tag="PC407_000905"
     CDS             11363..11563
                     /locus_tag="PC407_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HDF2496366.1"
                     /translation="MENLMEYLPLLIPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            11563..12480
                     /locus_tag="PC407_000906"
     CDS             11563..12480
                     /locus_tag="PC407_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2496367.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLEPDSGTIDVCGEKVTYGRTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIKIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNNEIQKFKSSGGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            12477..13235
                     /locus_tag="PC407_000907"
     CDS             12477..13235
                     /locus_tag="PC407_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2496368.1"
                     /translation="MREYIAFTKKEFKENIRNYKLLSLTILFLVFGIMSPLSAKFMPD
                     LIAHFAPTLKVTTAPTALDSWTQFSGNISGLGTSLTLIVFCNILSNEYSKGTLVIMLT
                     KGLSRSSVVLSKFSIAVIIMTIGFWLSFLCTYGYTMYFWPTANLNHIIFSAFNLWLIS
                     IMYISILILGCVLFRPAFASVLLVLAATAILSLISIPKQIAPYTPNFILSKNIDLVSG
                     KVTPPEFIIPIIVTIVISIVCLLLAVVLFNKKAV"
     gene            13618..14691
                     /locus_tag="PC407_000908"
     CDS             13618..14691
                     /locus_tag="PC407_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2496369.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            14761..15024
                     /locus_tag="PC407_000909"
     CDS             14761..15024
                     /locus_tag="PC407_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496370.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            15230..16207
                     /gene="bioB"
                     /locus_tag="PC407_000910"
     CDS             15230..16207
                     /gene="bioB"
                     /locus_tag="PC407_000910"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDF2496371.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMVFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            16435..17448
                     /locus_tag="PC407_000911"
     CDS             16435..17448
                     /locus_tag="PC407_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2496372.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNSAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDVSVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            17490..18380
                     /gene="rbsK"
                     /locus_tag="PC407_000912"
     CDS             17490..18380
                     /gene="rbsK"
                     /locus_tag="PC407_000912"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDF2496373.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIKVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKKLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            18428..19390
                     /locus_tag="PC407_000913"
     CDS             18428..19390
                     /locus_tag="PC407_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2496374.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            19444..20955
                     /locus_tag="PC407_000914"
     CDS             19444..20955
                     /locus_tag="PC407_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2496375.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            20939..21937
                     /locus_tag="PC407_000915"
     CDS             20939..21937
                     /locus_tag="PC407_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDF2496376.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            22121..23383
                     /locus_tag="PC407_000916"
     CDS             22121..23383
                     /locus_tag="PC407_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2496377.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIGGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFQMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            23403..24146
                     /locus_tag="PC407_000917"
     CDS             23403..24146
                     /locus_tag="PC407_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDF2496378.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            24319..25704
                     /locus_tag="PC407_000918"
     CDS             24319..25704
                     /locus_tag="PC407_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2496379.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFEYTPII"
     gene            complement(26041..26685)
                     /locus_tag="PC407_000919"
     CDS             complement(26041..26685)
                     /locus_tag="PC407_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2496380.1"
                     /translation="MNNLDKNNVNEVLDMLLRTRRSIRSFTKEVPSKGLIEEIIETGR
                     LAPYAILSVAKQTDFRHFFVISNNSLQMNKIKTILVDVIKTQISVNEKKAKNNITLQS
                     FITSLKMQVEKGVLGIGTAPYLIIVAERRGIPPIEQKSLSHVMQNMWLKSTALGLGFE
                     LVSVIGRLSDNKDFCDILGVPTGEYGFDACAIGFPTTSVTREGRAIPHPSIKWI"
     gene            26949..27563
                     /locus_tag="PC407_000920"
     CDS             26949..27563
                     /locus_tag="PC407_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDF2496381.1"
                     /translation="MKKVSIDRRTIRTKKMIRSALAELIDEKGFNNISVTDLTQRADI
                     NRGTFYLHYVDKYDLLERIENEIIQEIEEETKHLDSSNMMEIDTSSEPLPFMVKLFEY
                     FRKNSVIIKSILGPKGDPTFERKIKKFIETNLFEKQKLINFNIDEAIISEEYFIQYIL
                     SADLGVIQYWLEKNMKESPEEMALILARMSLLGPLRAVGIRKDS"
     gene            27784..27924
                     /locus_tag="PC407_000921"
     CDS             27784..27924
                     /locus_tag="PC407_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496382.1"
                     /translation="MKNENISYLALGLCFGVVLGSLFKNLAIGLSFGLVIGVVLDSKK
                     NK"
     gene            complement(28154..28601)
                     /locus_tag="PC407_000922"
                     /pseudo
     CDS             complement(28154..28601)
                     /locus_tag="PC407_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599264.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            29371..30210
                     /locus_tag="PC407_000923"
     CDS             29371..30210
                     /locus_tag="PC407_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496383.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            30200..30643
                     /locus_tag="PC407_000924"
     CDS             30200..30643
                     /locus_tag="PC407_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496384.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            30654..31145
                     /locus_tag="PC407_000925"
     CDS             30654..31145
                     /locus_tag="PC407_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496385.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(31347..32075)
                     /locus_tag="PC407_000926"
     CDS             complement(31347..32075)
                     /locus_tag="PC407_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2496386.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            32200..33288
                     /locus_tag="PC407_000927"
     CDS             32200..33288
                     /locus_tag="PC407_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496387.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLIIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKELMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            complement(33726..34574)
                     /locus_tag="PC407_000928"
     CDS             complement(33726..34574)
                     /locus_tag="PC407_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496388.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMSNNMNSNMGMPNNMPNNMNSNM
                     GMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            34773..35138
                     /locus_tag="PC407_000929"
     CDS             34773..35138
                     /locus_tag="PC407_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2496389.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            35161..37548
                     /gene="cadA"
                     /locus_tag="PC407_000930"
     CDS             35161..37548
                     /gene="cadA"
                     /locus_tag="PC407_000930"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDF2496390.1"
                     /translation="MEANNLIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLIVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENASSKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFKEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELVPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGESSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKFNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKAIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
BASE COUNT        14783 a         3959 c         6827 g        12419 t
ORIGIN      
        1 gatgatattt ttttagcttt atactataag gtatataaaa ctaagcctat taaaaatgtt
       61 ataatttcta ttaccatcat agaccatatc aatccatcaa gttcataaaa gtaattaagc
      121 tttattaaat agcttcttta ttataatttc tttatttcat ataatataaa tagtattaaa
      181 atttttatgg gagttaaagt aagcatgtat tattacttag atatttgtat aatcaacatc
      241 tagtaattta cctttttggt gaaaatgatt aataagtttt ttaggataat tcattattaa
      301 ttcatctgga aaattaattt cctttaagat attctcagaa tactcatagt tacctatgtc
      361 aaaacaaatg tgagcatctg aagttagtat gattttagca ccatattttt tgcaaagtgt
      421 agcaatatac ttacagttat ccgagctacc atttcttgac acgcctttaa tagaacaatt
      481 atttatttct attaagatat tcttttctat agctaattta atgattaatt catagtcgat
      541 tgggtagttt gggtttccaa ggtgtcctaa tatttcaata ttatcatgct tatttattgc
      601 atttattaaa gctttagtat tattctctaa tgtatttgga gaaaaaacag cttcgtgaaa
      661 tgacaagata agataatcta accgaggaat tacaaaaggc tctaggtcta tgtttccttt
      721 aatatctagt atatttgctt cacagcctct taaaattata atgttattta tgattcttgg
      781 gatattttga atgttgtgaa agtaccatct gtggggagaa ccaggcatct taggaccatg
      841 ttcagatgta cctagtatct taattccatt ttctttacaa taattaatat tttctagtaa
      901 agtgctatag ccatgaccac tgactataga gtgggtgtgt aaatctgata aaaaattcat
      961 ataaattcct cctctataaa agatgagggt aacgtaattt atattataaa acgaatagga
     1021 aagaaaatca atagttacat aattagacaa aaatattaaa atattgtaga attaagctta
     1081 ataataaatg tttaacagta ttactgctta taggggaggt taactatgac acaaaagaag
     1141 ctatcaagga aagaaaaagt tttaatttgc ttgaaaaggt taacagaaga acacttgaac
     1201 tttaataatg gcattagatt tggatttgat gcagaatata tagctactga agtaggtata
     1261 aatagaagta atgctagtaa ggaattaaat caattaataa aagaaagtat tattataaag
     1321 ataaagggta agcctgttca gtacttatat aaagaaagta ttgagagtat tttgggaaag
     1381 aaactaaata aagggatatt tgataatatt aactctataa acgaatatag tgaaaatgat
     1441 tctatcaaag atgagtatat tttagagagt ataaaaaaag agaacaatat ttttaattta
     1501 gtaggatgtg atggaagttt aaggtcccaa atagaacagg ctaaagcagc agtaatatat
     1561 ccacctaagg ggttgagtac attaataata ggacctacag gagttggtaa atctatattt
     1621 gcagaatata tgtataagta tagtttaact ataaaagaag atagtgataa tgcaccattt
     1681 atcatattta actgtgctga ttattctgat aatcagcaat tattattagc gcagcttttt
     1741 ggatatgtaa aaggagcatt tactggagct gataaagata agtatggtat tgtacatgaa
     1801 gctaataatg gagtattgtt tttagatgaa gttcatagat tatctgcaga gggacaagaa
     1861 atgctatttc tgcttatgga taaagggata tatagaaggt taggagaggc taataaggta
     1921 cacgagtgta gagttatgat aattgcagcc actacagaag acccagaaac agcaatgttg
     1981 gaaacgtttt taagaagaat acctgtaact ataaagattc cgtctttaga ggaaagaggc
     2041 tttcaagaga gaatgaagct tatatgttat ttttttaaag aagagagtgt taggattggt
     2101 gttaagttaa aggtttcaaa agaagttatt aaggcattta ttttatataa gtgtaaggga
     2161 aacataggcc aattaaaaag cgatatacaa ttaatttgtg caagagcttt tttagattat
     2221 atgacctata aaagggaatg tgtatatgta aggctctctc ttctaccaaa taatattaga
     2281 gaatcattat atggaaataa tagaaaagag gagtttgtac aaaatttcag ttttattgga
     2341 aaagatgata ttattttttt accagaagaa aaggattttg aaagtgaaaa tttattaata
     2401 gaaaataaaa aatatgattt agatttttat ggaatgataa aagaaacttg gaataaactt
     2461 caacaagaag gtattaaaga gtcacaaatt agaagtgtta tagatggaga catacagaag
     2521 tattcctata atttaatgaa tacttttata tataataata gtaatcaaac agcttataac
     2581 aacattgtta atgattcaat tgcaactact gtaaaatata ttttacaaaa gcatgataaa
     2641 tggagcaaaa gagaaggatt agataaatta ataaaagcta tagccttaca tattcagaat
     2701 ttaattgaaa gaataaagat tggaaatata gtaaagcatc ctaataaaga tgaaattatg
     2761 aaggaaagag catatgaata taacatagca aaagaaatat tatctaacat gtcattaatg
     2821 tatggagtag agtttccaga agatgaagca atttttttag caacatttct ttatttatcg
     2881 tatgtgggat taaatgagga gagtatagca atattagtca taatgcatgg tgaatctaca
     2941 gcatctagca tggtgaatgt agccaatact ttactggatt gtaaccatgc agtggcaatt
     3001 aatatgggat tagaagatag agttcaggat attttagtac aagctgtaga aatagccaat
     3061 aaaattgata aaggaaaggg aattttaatt ttaactgata tggggtctat tttaacattt
     3121 gctaaagtta ttcgtgatgc tacaggaaaa gaagtaaaag cagtagatat ggtaagtaca
     3181 cctattgtaa tagaagcaac tagaaaggct ctaactccag aaatgacatt agagaaatta
     3241 tattttaata ttatagaatc tataaagaaa cattatggag atatggaagt tacatatgct
     3301 gaaaaagata atggtggtag atattttgat agactactaa ttgataatat tagtaaaact
     3361 ttgacttttt taaatgggga aaaggcttat tttatattaa aagaagtaat taatagaatt
     3421 agtgaacact attctttagt aattcaagat gaacttttag taaagtttat atttcattgt
     3481 tcatgtatga ttgaaagagt aataataaaa gagtctttag tttataaatc atatgaagaa
     3541 agaattagta gaaataaaga attatattta gttataaaag aaagttttaa gctagtagaa
     3601 gagacattcg atataattat ttcagatatg gaatatgcaa atatattaga tatatttgaa
     3661 tcacaatatg atacagataa attcaaaagt tgatacaaaa tttaaaaata gccaaaaggc
     3721 tatctttttt ttactttttt tgatacagat ttgatacagg ttgaaaaaat cttttgatac
     3781 atatcgaaat aataagtttt taaaaaaaat tgagtaaaaa gttttgatta aatcctgtaa
     3841 aatagccatt tattattgat acacataagt tggcacggca attgcttata taaaaagtga
     3901 caagaaaagt gaggtgaaat tggtgatagg aataatagtt atgactcatg ggagttttag
     3961 tgaagaaatt ataaaaagct gtgaacttat tgcaggacca gcagaaagaa cagcagcaat
     4021 taaactaaat aggaatgata atattgaaga cttaaataaa aattttgtag aaaagttaaa
     4081 tgaattagat gaaggtgacg gagttttagt attagctgac ctattaggag gttcgccaag
     4141 taatgttgca agtttaaacc ttaaaaaagg tggcaagttt catgcactta ctggagtaaa
     4201 tcttccaatg ctacttgaag ctttaattaa tagagaggga aaaagtttag aagagttagc
     4261 agaagcatgt attgaagcag gacaaactgg tataaacaat attaataaag ttcttagctc
     4321 aatgtaatag aaaaaggagt ttgattaaaa atgtctttag taaaatattg cagatgtgat
     4381 gatagattaa tacatggtca agtgatatat aaatgggtta agcatctagg tgtaaaaaag
     4441 atagtagtag tagatgatga aacaaccaat gatgttattg ctaaaggttt gataaaaatg
     4501 gcagcaccaa agaatattga tttatcaata ttaactgtaa gtgaatcaag gagatatttt
     4561 tataataatc aagctgatga taacgttttt gtattaatta aaaatttaga tactgcaaat
     4621 agaatgatag aagaagggat taatatcaaa aaacttattg ttggaagaat acctacagga
     4681 ataggaaaaa agaaaatatc tcaaaatgta tacattaata aaaaagagtt tcttttaata
     4741 gatgaattta ttaaaaagaa tattaatgta tcaattcaaa tggtacctga tgaagaagaa
     4801 gtagaattaa accaaaatat taatcgctat aaaggagagt ttatttagca atgaataaat
     4861 ataaggtaat tatactgtgc catggaactt ggggaaaagt cttagtagaa gaaacaaata
     4921 aaaactttgg attgtcaaat caatatgaag ttttatcact aagttctgaa aaagatgttt
     4981 ctgtatttat gaaagaggtt gaagaagtag tagataaaga agatgtgaaa attataatat
     5041 cagatttata tggaggatct acatcaagtg ttgcaatagc tgttgctata agaagaggaa
     5101 tagatgcaat aaatggactt agcttacaga caattttaat cgtagatgaa gaattaagca
     5161 aagaagataa attacataca ttaccagaaa gaatcgtcaa aaaaaataat aacctttgtg
     5221 tggatttaat taaagaattt aaaaaaatat aaattatgag gtgaatataa tggcaaaaat
     5281 aacattagta agagtagacc acagattaat tcatgggcaa gttataacaa aatgggtaaa
     5341 aatcgctcaa gctcaaaaaa taataatagt tgatgacttc ttaggtcaag atgaatttat
     5401 ggctgacata tataaaatgg cagcaccatc aggagtagaa gtagttattt taacagcaga
     5461 agatgcggga caagcattcc aaaacaacac tttaggagat aaaaatatat ttattctatt
     5521 taaaaatgtt gatatggcaa ataaggctta taaagctgga cttaaatatg aaaagattca
     5581 attaggaggt attccaaatg aagcaggaaa gaaaatggtg tttacagctg tttcattagg
     5641 aaatgaggat gtagaacaac ttaatgaact taatgaaaat ggggttgaaa tagtcctaca
     5701 agttatacca gaggaatcat ctatgacata tgaaaatgcg ttaaagaaat ttaaataaaa
     5761 aatataaatt aggaggaatt ataaatggaa tctgttttga tgttggcaat tgtaacagga
     5821 ttatggtatt ggtttgcagc aggtcttgca gggtataccc ttttctcaac actaaagtct
     5881 cctttattta taggttttag ccttggatta ctttggggtg atgtaacaac tggtatgatt
     5941 gttggtgcaa gtatagaaat ggtttatcta ggaatggtcg cagcaggggg aaatattcca
     6001 tcagataaat gtctagctgc tcttattgca ataccagtag cacttcaaac tggtgttaat
     6061 gcagaagttg cagtatctat tgcggtacct ttaggtgtta taggagtatt agttaataac
     6121 ttaagaagaa ctggtaatgc tgtattggtt cataaggcag ataagtatgc tgaagaaggt
     6181 aatacaaaag gcatctggag atgtgcaaca ctttattctt taatatttgg gtttgtttta
     6241 agatttccaa tagtttttgt atgtaacttt tttggagcag atttagttca atcattatta
     6301 gatgttatac cacaatggtt aatgaatgga ttaacagtaa tgggcggtat attacctgca
     6361 ttaggttttg caacaacaat atttactata ggtaaaaata aattcttacc aatgtttatt
     6421 ataggatttt ttatggttca atattttgaa atatcaatta ctgcagctgc aatatttggt
     6481 gtttgtatag ctcttttaat tacctttatg aaagaagaca aaagggtagg tgaagtataa
     6541 tgcaagaaat aaaaaataaa gaagttaaaa atggacaagg tccaactgaa gttaataaga
     6601 caagagtact cacaaagaaa gatgtaacaa aaacatacct aagatggtgg tggacagcag
     6661 agttatctaa ttcttttgaa agaatgcaag cattagcagt ttgtgcaagt ttcactccgg
     6721 ctttagaaaa gttatataag aaaaaagaag atttagtaga tgcattaaag agacacttac
     6781 aattttttaa tactcaagct atatggggag gattaattca tggtacagtt ttagctatgg
     6841 aagaagagaa agctactgaa ggaaaaatcc caggagaagt tatttcaggt gttaagaatg
     6901 gtcttatggg acctttggct ggaattggag atactttaga ttttggtaca tttcaaacaa
     6961 tattcttagc tttaggtgct tcatttggag cagaaggaag tgttataggt gcatttttcc
     7021 caataatgtt tagtatatta ctattttgcg aaggttatta cttatttcat ttaggatatt
     7081 ctttaggtag agattcaatt aagaaaattc tatcaggtgg tattgtcaat aagataattg
     7141 atggtgcttc aattctagga atgtttatga tgggagcatt atctgcaact acagttaagc
     7201 tttcaactcc gctatcattt gatattggtg gtaaagcaat tgttgttcaa gatacattaa
     7261 atatgattgc tccaggttta ttaccacttg gtgttgtatt cttcgtatat tggggaatga
     7321 aatacaagaa gtggacaata actaagttat tagtaatttt agttgtatta gcattggtag
     7381 gatcattcat tggaatattc taattcacag tgattaaaat atagaattta attttaggag
     7441 gagaaagaaa tgtcagaaaa aatgtataca gaaaaggaat atttagaagg aattagagga
     7501 gcaacaggag aaagagcagt atggttctat cttcttatga aagaagctga aaaattagga
     7561 gctaatccag atgatatatg taaagaagca atatatggat ttggtaaaat gagaggtcaa
     7621 aaatataacg tagcagatac tccaggaaaa atggcagaaa tgttatataa cagtaaagga
     7681 caaaaagtgt ttgaaatgga attagtagaa aatacagatg agaatggcgt tttaaaattc
     7741 catcactgtc cattagatgc tgcttggaaa gaatatggat taactaaaga agaaagaaaa
     7801 gaaatttgta gattagcttg ttatggggat tatggaagag ttgactgtgc acaaggtgtt
     7861 aaattagatt ttgctcaaaa atgtgctcat gatgatgaag tttgtgaatt agtatttaca
     7921 aaaaaataat ttttaatgct aaaagcctgt tctttaagga caggctttaa aaagatagaa
     7981 agtaggttga tttatggata taagcagaag tgtagataat agtttaaata gaaccataga
     8041 atttctaaaa gaattgataa aaattgattc acaacaaggt gaacctattt cacaatgtcc
     8101 ttttggtata ggacctaaaa aatctttaga taaaacttta gattactgtg ctagtttagg
     8161 ttttagtgtt aaaaatattg ataattatat tggatatgca gaaattggag agggagagga
     8221 attaataggg attccaatgc atttagatat agttccacca ggagaggggt ggagtgtaga
     8281 cccattttcg ggagcagtaa ttgacaatat tatttatgga agaggtgtca tagataataa
     8341 gggtgctgta tctatgctta tacatgtatt gaaaaacata gaggatatgt atcctacaat
     8401 taataaaaga ataagattaa tttttggtac aaatgaggag acagatatga aatgtatcaa
     8461 atattactta gataaaggtg aagaaattcc aagtatggga tttactccag atgctatgta
     8521 tccagtggtt aatggagaaa aaggaagggt tcatataagg atagaaaaag aaattaaaat
     8581 tgacaaaagt aaaccttaca taatagtaag gggaggaact aaagaaaatg ttgttccatc
     8641 tcattgtact gcaaaaatta ttaatggaat tatttcagag ttaactacta aaggagtagc
     8701 tgtacatgca agtaatcctg aaaagggaga aaatgctata tctaaaatgg taataaagat
     8761 agttgaggat aatatggatt ttcagcatag agaagatata gaattagttt caaaatacct
     8821 ttgtagtgat tattatggag atgcattggg gattaatcaa tatgatgagg tattcaaaaa
     8881 cactacctta aatttaggaa tattaaaagt aaatgaggaa aaaatcgtat gtgagttaga
     8941 tataaggtat ggtaaaaata tagttttaaa taacataata gatagattca aaaaggtttt
     9001 ttgtaatggt tggaaaatag aggttattgc tcataaagac ttacattatg tggatgagag
     9061 taatttagta cttaaaaaat tattagaagc ctatgaagaa gtaacagatg aaaatggata
     9121 tacaattgct atgggtggtg gcacatatgc aagttggttt aaagatatgg tggcgtttgg
     9181 acctaagttc ttagcgtata aaactggtgg tcatggagta gatgaaagag taccaataaa
     9241 tcatatacga aagaatatgg agatatatac tttggcttta ataaaactat tagaattgta
     9301 aaagaagaag agactttatg tgcaaattta cagatatctt aaaaagagag ataaaacgag
     9361 ttcaacttta tttaaatgtt caacaccatc aaatggggct gtctcaaaat agagataagc
     9421 ctcttgctta ttattgaaat gatgaaaata tattttactt catacaaaaa agagtgcctt
     9481 atgaagtaaa aagttaattt tgagacaccc tcatttgatg atgttgaaga gaaaataagt
     9541 atgaaattat taaaaaaaga tgattttata aaagttaagg ttgaaaataa aagttaattg
     9601 aatggaatta gataaatgga gaaggctgta tactatctaa taaaataaca aaagagtgtt
     9661 ataaaactgg ttatatgatt agagaaaagt ctattgatac ttatccagat agtggacggg
     9721 gatttatgac ttgggatgaa aatggagaat aataaaaatt atagtttata caatttaata
     9781 agatatatgt acaagcagtt aatctaaatg attgactgct tttcttttat agatttttat
     9841 ataatagaca attactaata catgatagtt gcctgttata attagaggaa tagatacgaa
     9901 taataacttt ataaaagaat taaatggaaa attgaagagt tatattcagg attttaaaaa
     9961 gaatatgaaa tgagaaatga aaataaggtg tttatattaa aagaggtttt acacaaaata
    10021 cttaaaaacg tgataaataa aaactgtatt tagataaatt aactactagt tttatatata
    10081 atatggattt tgtttacaaa gtttacatat ttcacaaaat atttttaaaa tatatttaaa
    10141 aatatatttt atatacttgt tcgtttgttt ctctgtggtt gattttttcg tcaaaagtca
    10201 acttcaaatc gcttgttagc tttacatgtt taaggatata attatacata aggagggatt
    10261 gacatgaatg aattaaacat cgcaaaaacg ctgattttaa agcgtaaaga aaaaggaatt
    10321 acacaagatg aacttgcaaa ttatataggc gtttctaaag cttctgtatc taaatgggaa
    10381 actggacaaa gctatcctga cataaccttt ttaccgcaac tcgcgacata tttcaacatt
    10441 accgttgatg agttaatatg ttatgaacct caaatgatga aggaagatat taataaactg
    10501 tataataaac tgtgcaaaga ctttacggtt aaaccgtttg atgaagtcat ggatgaaata
    10561 agagaaatca taaaaaaata ttattcatgt tttcctttga tttttcggat gggtctttta
    10621 atagttaatc actatgatat tgtagatgaa aaaaagcgtg aactattaat tagtgaagca
    10681 ttagaaatat ttattcgtat acaggaaact tgtaatgaca tagatatttg ccgtcaagca
    10741 aaaagtatgg aggcaacttg ttatatattg ttaaatcaac ccatacaagt gattgattta
    10801 ttgcaaaaca gcaattttcc catgataaat gaatctatac ttcttgcaca ggggcaaatg
    10861 atgaatggtc aaatggatga agcaagagaa acatttcaac ttggtgcata tcagaacctt
    10921 atatcattag tccaaaatct agtgggtatt ttgcaaaatg cagataaatt acaaatgaag
    10981 gaaatagaaa gacgtattct agcaatttca gatatatttg agcttgatac attatctcct
    11041 gctattatgc tttcttcata tttaacacag acacaaatta atttaataca tggggataat
    11101 gaaggagcta ttaaatcatt acgaaaatat gtagatttag caacacgtga tatataccca
    11161 attataatac atggggatga cttttttaat aaacttgaca gatggatttc tgaaattgga
    11221 acaggtatta cacgtgatga tacaatcgta aaagcaggta ttgttgctgc tattaaaaat
    11281 aatccaatgt tttctttatt aagtgaaaac aaagaataca aatttcttat agaaaaatta
    11341 tctttattgg aggaataaga ttatggaaaa tttgatggaa tatttaccac ttttgattcc
    11401 tgttattatt ttagatttaa tacttataat tactgcactt gttcatgtat tgagacatcc
    11461 aaattataaa attggaaata aggcaatatg gattattgta gtactgttta ttagtctaat
    11521 aggaccaatc ttgtatttta caataggaag aggtgaagaa tgatgaatat tgtctcaata
    11581 aatggattat caaaaggttt tggtaatcga aagataatag ataatctaaa ttttactgta
    11641 ccagaagggt cagtctttgg gtttgtaggt aagaatggtg caggtaagac tacaacaatg
    11701 aaaatggtac ttgggttact tgaaccagat agtggaacta tagatgtttg tggtgagaaa
    11761 gtgacatatg gaagaacaag ttctaatcgt catgtaggtt atctgcctga cgttccagag
    11821 ttttacaact atatgagacc acttgaatat ctttctcttt gtggtgaaat tacggggctt
    11881 tcaaaaaaag aaataaagat aaggagtgaa gagttgttat cacttgtggg gcttggcaat
    11941 gaaaagagac gaataggtgg gttttctcgt ggaatgaagc aacgacttgg tatagctcaa
    12001 gctttgcttt cacgtcccaa attacttatt tgtgatgaac caacaagtgc acttgaccct
    12061 gttggtcgaa aagaaatatt agatattatg cttaaaatca aagactcaac aactgtaata
    12121 ttttcaacac atatactatc agatgttgaa agaatctgtg accatgttgc tatactaaac
    12181 aaaggaagca ttgctcttag cggtacactt tcagaaataa aaagtatgca cggaaaggat
    12241 agacttttac tggagtttgc aagtaataat gaaattcaaa agtttaagtc tagtggtggc
    12301 attaaatcct tactaaaaga ttcagaagaa actaatatgg aaattgtttt gcatggtaaa
    12361 gatataaaag caatacaaaa aacagttata tctacactcg cagaaatgaa tctttgtcca
    12421 gtaaagatgg aactaataga attgtcactt gaaaatttgt ttttggaggt agtaaagtga
    12481 gagaatacat agcatttaca aaaaaagagt ttaaggagaa cataagaaat tataaattac
    12541 ttagtttgac tattttattt ttagtatttg gaataatgag tcctctttct gcaaaattta
    12601 tgccagactt gattgctcat tttgctccaa ctttaaaagt tactactgca ccaactgctt
    12661 tagattcttg gacacagttt tctggaaata tttctggtct tggaacgagt ttaactctaa
    12721 ttgttttttg caatatttta tcaaacgaat attctaaagg aacactcgtt attatgttga
    12781 ctaaagggtt atcacgttca tctgtagtat tgtctaaatt ttctattgca gtaattatta
    12841 tgacaattgg tttttggttg agctttttat gtacttatgg atatacaatg tatttttggc
    12901 caactgcaaa tcttaatcat attatatttt cagcatttaa cttatggtta attagtatta
    12961 tgtatatttc catattaatt cttgggtgtg tactatttag accagcattt gcaagtgtgt
    13021 tacttgtatt agcagcaact gctattttga gtttgatttc tattcctaaa cagattgcac
    13081 cttatacgcc taatttcata ttatcaaaaa atatagattt agtttcaggt aaagtgacac
    13141 ctccagaatt tattattcca atcattgtaa caattgttat ttcgatagta tgtctgttat
    13201 tagctgttgt tttgtttaat aaaaaagcag tttaaggata tttttattat aaatacaaat
    13261 agtgctgtat tgtgggaaaa tgtgtttagt attgtagaag aatatattta agtaaaaaag
    13321 aggctgtctc aagatagaga aataagcctc ttttttattt tagtcctcct cactatactt
    13381 tataataatt aagttattac cttaaggaac tatctaaaaa aagagatata gattataatc
    13441 taatgcttat cagttatttg aggtgtttag tatatagagt ctaaagtagt gaaggactaa
    13501 aataatattg ttttcattaa ataaagataa gaatctttaa ttgtggtaaa atatactaaa
    13561 aaatcatttt aaaagtgata aaattatttt aagtaaatat tttaaagggg tgtagatatg
    13621 gaaaagtcaa aagtatattt ttgtgattta tactcaaatt cgcaaaataa aaacgtacca
    13681 aacaatgtaa gaagattatt tgatgaagca ggattcaaag atttaataga aaagaatgac
    13741 caagtcgcaa ttaaacttca ctttggagaa aagggaaata ctacatatat gagtcctgtg
    13801 gcagttagac aagtggttga taaggttaaa gactgcgaag gaaagccatt tttaacagat
    13861 acaaatacac tgtatacagg gagtagaaca aattctgtag accatcttac aactgctata
    13921 gaaaatggat ttgcttatgc agttgtaaat gcaccagtaa taattgcaga tggattatat
    13981 agtagaaatt atgagaatgt aaaaatagac aaaaaacatt ttgaaagtgt aaaaataggt
    14041 ggagaaattt ataattcttc tgcaatgata gtaatgagtc attttaaagg tcatgaagca
    14101 gcaggttttg gaggagcatt aaaaaactta gctatgggat gtgcaagtgc agcaggaaaa
    14161 caaatgcaac attctgatgt tactccagta gtaaaggaaa agaagtgtgt aggttgtgga
    14221 aagtgtgtta attcttgtcc tacaaaagct ataagtatag tggataaaaa agcagttata
    14281 gattctgatg tatgttatgg atgtggagaa tgtccaactg tctgtccaac tagagcagta
    14341 actatacaat gggaaagtga ctcagatgtg tttgtcgaaa agatggcaga atacgcttat
    14401 ggagcagtat caaataaaaa agacaaggta gggtatataa cttttgttat gaatgtaact
    14461 ccactttgtg attgtgtacc ttggtcaggt agaccaattg cccatgatat aggaatatta
    14521 gcatcaacag accctgtagc tattgaacaa gcatgctatg atttgatttg taaggaaatg
    14581 ggtcatgatg ttttcaaaca tgaacatcct catgtaaatg gaactagaat aatagactat
    14641 gcatgtgaaa tgggtatggg aagtaaagaa tatgaattaa taaaattata agaatagtag
    14701 cacttaaatg catataatta ttgtaagaga aatctaaaag tatcaaagga gtatgcattt
    14761 atgaaaattt actatgagaa ggaaaacagc aagaaggcta atgtaaacaa tattccaagt
    14821 gtatttgcgt ttaattttct aagaaacttt ttgactagca aagagtatga ggaaactaga
    14881 aaagaatatt ttataaataa tctaacaaaa agtcaggtta atcaagccat gaaggattta
    14941 aaatggctat ttagagaata tgaaggctta gaggttatta caatggaaag cttggatgga
    15001 gttaagacaa ggatagtttt ataaaccagt aagaattata ttctaataaa aaatactgct
    15061 ttaataataa tatgtataat ctatatacta atattatttt gaagcagtat ttttttgtgt
    15121 atagtaaact tgtgtctaaa ataaagttga ccaataaatg ttatatatgc aatactatat
    15181 ataataagta aactacataa aaatattaag ttgacaaata ggaggatata tggaaaaata
    15241 cataataaaa ttaaaaaata aagtattgag agaaaaagaa ataagttatg aagaagctct
    15301 taatttaata agccttgata caaataataa aaacgatttt gatattctat taaaatctgc
    15361 aaatgaaata agagaatatt ttatgggaag aaaagcagat ttatgtacga taatgaatgc
    15421 taaatctggc aaatgtagtg aagactgtaa attttgtgct caatcatctc attataaaac
    15481 tggtgtagaa gaatattcat tattagatta caatgaaatc ttaaatagag ctaaagagat
    15541 ggagagtaaa ggagtgcata gattctcatt ggttacaagt ggtaaaggta tgtcaggaaa
    15601 ggagtttaat aatattttaa atatctatga aggattgagg gaaaatacta atttaaaatt
    15661 atgtgcatct ttaggtatta tagattatga gcaagctaaa atgttgaagt cagcaggtgt
    15721 gactacatat caccacaatg ttgagacgtg tagagataat tttcataata tatgtacaac
    15781 acacacatat aaagatagga taaaaactat aaaagatgct aaaaaagctg ggctagatgt
    15841 atgtgtaggc ggtataattg gaatgaatga gagtgaagaa cagagactaa aaatggtttt
    15901 tgaaatacga gaattaaatg taaaatcatt tccaataaac attttaaatc ctattaagaa
    15961 tacgccaatg gaaaattatg atgtattaga accaatggaa atactaaaaa caacagcagt
    16021 atttaggttt ataattccta atgtgtacat aaggtatgct ggtggaaggc tatcattaaa
    16081 gggatatgac aaagttggat ttaatggggg agtaaattct gcaattgttg gtgattattt
    16141 gacaactgtt ggaagtggga ttgaaaatga taaaaaaatg attatagagc aaggatttga
    16201 actttaataa atactactta aattaattaa tatactttta gataattata aaagtatatt
    16261 aatttttttt gcagattaat ttaaatatta aaattgaatt ataagttgca atattttgaa
    16321 aaaagggaaa aatatcaaaa tttatataaa atggttgcaa atgggaaaag gatgtgatag
    16381 tatctaggta accggttaag taaaacggtt tcctaaattt tgggggtgaa cttcttgagt
    16441 ataactataa aagaaattgg cgaattggct ggtgtttcta aaacaactgt gtcaaaagtc
    16501 attaataata aagatgaaaa tataagtcag gcaacaagag aaaaaatttt gaaaataatg
    16561 aaggaaaaaa attatgtacc aaataaatta gcacaaagtt tagtaactaa aaaaactaat
    16621 actatagggc ttcttatacc agatatacga aatccattct ttacagatgt atcaagaggt
    16681 gtagaagaca aagctaatga agaaggctac aatataattt tatgtaatac agatgaagat
    16741 gctaaaaaag aatatgaagg aataagaaca ctatcagaga ggatgataga tggaattata
    16801 tttgcagcat cttcaaatac aaactggaaa gaagcaaatt ataaagatat aaaaatacca
    16861 actgtcttga ttgataagaa gataagtatg aacaaagaaa ccttaaaagg aattgttaaa
    16921 ataaataact ttgaaggggc ctatatagct acaaaacatt tattagatat tggaaataag
    16981 aaaataatat acctaagtgg tccacttcaa aatgaaatag cagtagatag attagaagga
    17041 tataaaaagg ctctaatcga gtgtaattta agctacaatt ctgcttatgt atttgaagga
    17101 aaatataaga ttgaatgggg acaagaattt ataaaaaatt tagaaaagat agattttgat
    17161 gcaatatttt gtgcaaatga tttgattgca ataggtgtca taagaggtct taaagaaaga
    17221 gggttaagta taccaaatga tgtaagtgtc gtgggatttg atgatattca aacatcaagt
    17281 ctaataagcc cttctcttac cacagtaaaa cagccgtctt atgatatagg atataaagcc
    17341 agtgaaatat tgataaattg tttaagagga gataaaaaag aatcatttga tgaattgatt
    17401 ttcaaaccag aattagtcat aagagattca acaaaagaaa gtgaataaca gcacgagaga
    17461 cataaataga tatgtaaagg agtaatgtaa tgggaaacat agttgtcata ggaagtgtaa
    17521 acatggacat ggtatgttct gtagataaaa gaccagaaaa aggagaaaca gtattaggta
    17581 atagtttttt tacatcacct ggagggaaag gggctaatca agctatctca gcttctaaac
    17641 taggagcaaa tgtaaaaatg atatcatgca taggggaaga tggcttagga gaggagttaa
    17701 taagaaattt tagaacgaat aaagttgatt atagtttagt atccagaaat aatcacaaaa
    17761 gttctggtgt tgctgttata acattatgtg aaaatgataa tagtattgtt gttgtaccag
    17821 ggacgaatga gctagtagat atagaattaa ttaaaaagaa tgaagaagag ataaagaatg
    17881 cagatatagt attgctacaa ttagagattc cattaaaaac aataaattat gtagtgaatt
    17941 tctgttttga aaataggatt aaggttttat taaatccagc accagcagta aaactaaatg
    18001 aagatataat agaaaaagta acttacttaa caccaaatga acatgaatat aagatagttt
    18061 ttgacacaaa tgaagggata gaagaagtat taaaaaaata tccaaataaa cttgtaataa
    18121 cagaaggaaa aaatggagct agattttatg atggtgagga aatcaagcat gtatcttgta
    18181 taagtgttga tgttcaagat accacaggag caggagatac atttaatgga gcattggcag
    18241 tggctataac agaagggaaa aaattatata cagcagtaga atatgcagta gtagtatcag
    18301 gtctttctgt aactaaacta ggtgcacaat ctggtatgcc ttataaagaa gatgttgaaa
    18361 aatatttaaa taataaataa aaatctaatt tgttcaaatt taaaactatt aaaaatggag
    18421 gttaattatg agagtgagaa aaattttatc tttaggaatt gctggaatac tagcaatagg
    18481 aatgttaaca gggtgttcaa tggaaggacc aagtaaatca gataataaag gtggttcaga
    18541 caagaaagat ttaactattg gagtatctac aataactctt caacatcaat tttttataga
    18601 tattgatgag ggaataaaag aaaaagctaa ggaattaggt gtgaaagtta tagtaaatga
    18661 cccagaccaa gatgtagcta agcaaacatc agcaatagaa gattttatac aacaaaatgt
    18721 agatggaatg atagtacttg gaactgataa ttcggctata gtaccagcag tagaaggagc
    18781 atttgaaaaa atgccagtag ttacagtaga tgctgtatta aatactgaaa atattacaag
    18841 ttatgtagga acagttagtt atgatgctgg taaaaaatta ggggaataca ctaaaaaata
    18901 catagatgaa aagttaggtg gaaagtcaga aatagcaata gtgacagatt taaaatctca
    18961 aatacaaatg cagagaatag atggatttaa agatgcgtta aaaggttcag caaatgttaa
    19021 gatattaaat tcacaaccag gatatgacag agaagaatct cttaatacag tagaaaactt
    19081 aattcaatct aatccagatg tagatataat atatgcaaca gctgaaaata gtgtactagg
    19141 agctaaagct gcacttgaat ctgcaaaaaa taaggatgtt aagattgtag gatttgactt
    19201 aactgaagaa gcatcaagtg gaataacaga tggaactatt cttgctatga ttcaacaaca
    19261 acctaaagag atgggacgtt tagctgtaga agcagtagta aaagctataa aaggggaaaa
    19321 ggtagaaaag aatataccag taccagcttt actttatgat aaggaaaata taaaagactt
    19381 taagaattag taaaataaaa tgagattatt tgaaagaaat cctatttaaa aaggagtgtc
    19441 attatgagta atataattct gaagttaagt aatattgcta aagaatttcc tggtgtcaga
    19501 gcattagata atgttaattt tgagttattt catggcgaag ttcatgcact actgggtgaa
    19561 aatggtgctg gtaaatcaac aatgataaag attttaactg gtgcacattc gaaaacttct
    19621 ggaaaattta tttttgaagg caaagaaata gagaatatat ccccagatat ctctaaaaag
    19681 ataggtataa atgccatata tcaagaacta acagtttttg acgaattaac agtagcacaa
    19741 aatattttta tgggaaaaga aataaatggc aaagtcttaa ccaatgacaa aaaaatgaat
    19801 gaagaggcta aaaagatttt cgataatatg ggaatagata taaatccaaa tagtttagtc
    19861 aaagagctta gtatagctca aaaacaaatg gtagaaatag caagagtttt atcatcagaa
    19921 actaaagtat taataatgga tgaacctact tcttcaataa gtaaaaaaga gactgaaata
    19981 ctttttagac ttataaatga tttaaaagag agtggagtta gtataatata tatatcccat
    20041 aggatggaag aactctttga gatttgtgac agaataacta taatgcgaga tgggaaaacg
    20101 atttcgactt tgaataccaa agatgtatcg tctgaggaag aattagttaa tttaatgata
    20161 gatagaaagc tagaccaatt tttcccaaaa agaaaagtag aaataaaaga agaaattatg
    20221 cgagtggaaa atctaactaa gaacaatgtt tttaatgata ttagctttaa tataagaaaa
    20281 ggtgaaatat tgggaatagg tggactagtt ggaagtaaga gaagtgaaat agtagaagct
    20341 atatttggac ttagaacata tgattctggg aaaatatatt taaacaatga agaagtaaag
    20401 tttaaaactc catcagatgc aatagaaaat ggactgggat taataacaga agatagaaaa
    20461 ggtactggat tgtttttaca aatgagtgtc aaagaaaaca caactatggc tgggttaaaa
    20521 aagatatcta agtttaaatc aattatagat agaaaaaagg aaaaagagat tttagaaaaa
    20581 tacatagaag ctttaaagat aaaaacacca catatgaatc aagttataca aagtttaagt
    20641 ggtggaaacc aacaaaaagc tataatagca agatggcttt tattacaacc agatatattg
    20701 attatggatg aaccaaccag aggtatagat gtaaatgcta aggcagagat atacaatcta
    20761 atgggagatt tagtggagag tggtgtaagt ataataatga tatcttctga aataccagaa
    20821 ttaatatcaa tgagtgacag aataatggtt atgagagaag gtcatataag tggattttta
    20881 gaaggtgaag aaatggttga aaataatgta ttaaaattgg ctttcggagg taaaattaat
    20941 gagttcaata actaagcata aagaagaaaa atcttctcca ttattgacaa aattaacaag
    21001 tgtatttaga gaccaaggga gtgcagctgt aggacttgta ataattttta taataatgag
    21061 tgtagcttca tctaactttt taactttaga taatttaatt aatgttggaa gacaaatatc
    21121 aataaatgca atacttgcag taggtatgac atttgtaata attacaggag gaatagattt
    21181 aagtgtagga gcagtaattg ctttagtagg tacattttgg gctacaacag tagtgaatta
    21241 taatgcacct atatgggtag gtatgatact tgcattagca atagggacaa tattaggagt
    21301 cataaaaggt gcaattattt caacacaaaa actacctcca ttcattgtaa ctttagccat
    21361 gttaacgata ataagtgggg caagctttgt atttacagga ggaagaccta tatcagtaaa
    21421 tacagatgca tttaagatgc tcggtagagg atatattgga ccaataccaa taccagttat
    21481 aattatgatt atagttgtaa tagcaggaca ttttctatta aaaagaacta attttggacg
    21541 tcatgtacat gcagtaggtg gaaatgaaga ggccgctaga ttatgtggag taaaagtaaa
    21601 taaagttata gtaaaagtat acgcactagc tggactacta acagcattag ctgggataat
    21661 actatcatca cgtttggcat caggttcacc aactgttgga gatggagcag aattggatgc
    21721 tatagcagcc gttgtgcttg gtggtactaa tatgatgggt ggttctggtt ctatagtagg
    21781 tacatgtata ggtgtcggaa taataggaat attaggaaat ggattaaact tattaagtgt
    21841 atcttcatat aatcaaatga ttataaaagg tttggtaatg ttatttgcta tatggataaa
    21901 taatataaaa ttaaaaaaat cagctaaatc taaatagtaa ttaaatttta gcttataaac
    21961 tataaaaatt attaagtttt tgtatatatt tttatacttt aatttgcata catctttata
    22021 cattgaatgg gaatatgcta agtgaaaaag tttactatgg cttaaataga tgagattaga
    22081 cttgaaattt taaatgtaaa aataaatctt aggaggaaat atgaaaaaaa ttctcttaga
    22141 tacattaaat agtaaaaaac aagaatacat agattattta aaagaacttg taagtataaa
    22201 gactgaagat gttggtcatg gaatactagg tggatttgaa aaagaaggac aggaatatat
    22261 tgaaaaactt gcaaattaca taggttttag tgtagataga caagaaatga gcgaagaatt
    22321 aattaaaaaa gcaaaaaata tatataaaga aggaaatcta ggtcacaatt atcaagatag
    22381 atataattta atttgtaagt atagtgatga tttacctggt aagacaattg tatttaatgg
    22441 tcatgtagac actatgccac caggtgatat aagtaagtgg aaatataatc catatcgtgc
    22501 tacagaagat aatggaaaac tttatggtct tggaactgct gatatgaaat ctggacttat
    22561 agcatcaata ttagctgtaa agcttataaa agatagtggt ctaaatgtac ctggtaatgt
    22621 aaaaataatg tcagtagtag atgaagaagg tggcggaaat ggtaccataa atgcagttat
    22681 gaatggtatt ggtggagatt gttgcataat ttgtgaacca tcagaacaaa acttaatagt
    22741 tgcacatatg ggatttgtat tctttgaagt tgaggttaaa ggtgtgtctc ttcactgtgg
    22801 tagtaagtgg gagggtgtaa atgcaattga gaaggctatg ttgttgcttc aagatataaa
    22861 agagttggaa cataactggc ttatgatata taagcatcca ctattacctt cacctacaat
    22921 aaatttaggt gtaataaatg gtggaacagc aggttctact gtgccagata agtgtgtatt
    22981 taatttatgt gtacacttct tgccaaatat aatgagttat gaacaagttg taaatgatgt
    23041 aacaaatgtc ataatgacaa gggctaatgg agatttatgg cttaaagata ataaaccaaa
    23101 tataaacata tatcaatcag ggttaggttt tcagatggac aaggattctg attttgtagt
    23161 gaatgcacat aaaatactag aagaaacttt aggaaaaaaa ttagagataa aaggttcaac
    23221 agctggcaat gatgcgagat taatgaaaaa tttagctgaa ataccaacac ttatattagg
    23281 gccaggttca atagaacaat gtcattcaat tgatgaatat gttgaaatta aggagtattt
    23341 agatagtata cttatgtatg caagtctaat attaaactta taaaagtagg aggaatatat
    23401 aaatgaaaaa agtattaata gtaggggaat cttgggtaaa aaatataact catataaaag
    23461 gatttgatac gtttgttaca actcattatg aagaagctgt aaagtggttg aaagaagcaa
    23521 tagaatctgg aggatatgag acagttcata tgcctgcaca tgtagcagca gatagttttc
    23581 cgtataaatt agaagagtta aatgagtatg actgtataat attatctgat ataggttcta
    23641 atacgttctt attatctaat agtactttta tagattgcaa tagtaatcca gatagacttg
    23701 aactaatcaa agagtatgta aataatggtg gggcgttaat catggtaggt ggatatatgt
    23761 catttacagg aatagatgca aaagctagat ttggagaaac tgctatcaaa gatgtattac
    23821 caataacaat gatagataaa gatgatagag ttgaaaaacc agcaggaata attccagaag
    23881 ttattgactc tgaacatcca gtattaaaag gtataccaac agaatggcca aagtttttag
    23941 gatataacaa aacagtagct agagataatt gtcctgtttt agcaactatt ggaggagacc
    24001 catttgtagc tgttggcgag tttggaaaag gaaaatctgc tatctttagt tctgactgtg
    24061 cacctcactg gggtccaaaa gaatttactg attggaaata ctataataaa ttatgggtta
    24121 atatgcttga ttggttaaca tgctaaaaat tttataaaac ttgaaaacct cttacttgat
    24181 aaaatgaaac ttacaagtca agaggtttta ttgtataaaa tggttgtatg gtagaaaata
    24241 tttttaaatc agttaaaatt agtcattttg gctgataatc gataagttat tagaaacagg
    24301 gaggtttata taattaatat ggataattta aataaaaagt ttttgatgga atatatagat
    24361 gagatacagg gagaattaag agatatatca gataagatat ggaaaaatcc agaacttcag
    24421 tataaagaat actatgcttc aaatttacaa aaagaatgtt taaaaaaaca tggttttagt
    24481 atagagggat tagaaggtat ggatacagca tttgtggcta gttatggaga aggtagacca
    24541 gttatagcag ttttgggaga atatgatgct ttagatggtt tgtctcaaaa agtatccact
    24601 actaaagaac ctattgtaga tggtggtgca ggtcatggat gtggacataa cttacttggt
    24661 acaggttcaa taggagctgt aattgcagta aaggaattaa tcaaactaaa aaagctgaaa
    24721 gggactataa aatactatgg atgtccagca gaagaagatt tatctggaaa agtattgatg
    24781 ataaaaaaag gtttttttga tggaattgat tgtgctttta gttggcatcc atttgatata
    24841 aatactccga taagaatccc aacacttgct aattactctg tgaaatttag atataatggg
    24901 attagtgctc atgcagcaca agctccttat aacggaagaa gtgcacttga tgcagtagaa
    24961 cttatgaata taggttgtaa ttatttgaga gaacatatat ttgataattg tagagtacac
    25021 tatgtaacta ctaatggtgg aaaaatgcca aatatagttc ctgattttgc agaagtgtgg
    25081 tattacataa gaggagttaa aatggagcat gtaagagatg tatttggtag gattgttgat
    25141 atagcaaaag gagcagcatt aatgactggt actattatgg aatatgacat tataagtgga
    25201 gtatatgact atattccaaa tacaatatta acagatattc tatcacaaaa tatgaaatta
    25261 gtaggtgtgc aaaactataa tgaagatgac tactattttg cagataaatt agctgagaca
    25321 gtgtctatag ataaaagaag tagtgtttct agtgtattaa gtggaaataa ggatattact
    25381 aagatgaatt tacatgatga ggttacagat gatacattta ctcataataa ctgtatatca
    25441 ttatcattgg atattggaga tgttagctac ataattccta cagcacaatg ttcttgttca
    25501 gtttggccta ttggtatttc tgcccataca tggcaatctt gtgccagtgc aggttctgat
    25561 atgggattta aggcaatgtt attggcatct aaatctatat catgttcaat aaatgatgta
    25621 atgcttgatg aattggttat aaataaagca aaaaaagaat tgaaggatac tgtaggtagt
    25681 tttgaatata ctccaattat ttgaagagta atttaataca agtaagattg aattgtatga
    25741 attaggatga atatataaat taatattatt tttaacttaa ctttctaatt attttcaatc
    25801 taagatacaa taattagaat atatttttaa agtttcctat ttaaggaaaa taatttgatt
    25861 ttatgtttga taaaaggact tataatttgt aagccctttt attatattta ttttttgttt
    25921 ataattaatt ttatttttta ggtttagtta ggttatagat taccctgaaa aataacattt
    25981 aacttattat taaaaacatg taagaatagt ttataaatat tttattttcg atgttgttta
    26041 ttatatccat tttatcgatg gatgaggaat ggctctgcct tccctagtta cagatgtagt
    26101 agggaaacca attgcacagg catcaaatcc atattcacct gttggtactc ctaaaatatc
    26161 acaaaaatct ttattatcgc tcaatcttcc tattacacta acaagttcaa aacctaatcc
    26221 taaagcagtt gatttaagcc acatattctg catcacatga gataatgact tttgttctat
    26281 aggtggtatt ccacgacgtt ctgccacaat aataagataa ggtgctgttc ctataccaag
    26341 aacccctttt tcaacttgca tttttaagga agtaatgaag gattgaaggg taatgttgtt
    26401 ctttgccttc ttctcattga ctgatatttg agttttaata acatcaacaa gaatggtttt
    26461 tattttattc atttgtaaac tgttatttga aataacaaag aaatgtctga aatctgtttg
    26521 ttttgctact gaaagtattg cataaggtgc aagtcttcca gtttcaatta tttcttcaat
    26581 cagccctttt gaaggtactt cttttgtaaa acttcttatg gatcttctag ttcttaatag
    26641 catatccaaa acttcattaa cattgttttt atctaaattg ttcattttta tcctccacta
    26701 atagtattct taagatgttc tataatgcat ccatataaag gcgaactttt cggtcatatt
    26761 aaacatttcc ttcaaattaa gtcgctattt ctatgaacac aatgttgatt aataatctat
    26821 atgtatatta taatatttat attgcattat gcaataaaca ataatattta aactgttaag
    26881 taataaacat ttactataaa attgttaatt aaaattaaaa aaatttatat ggaatgagga
    26941 aataattcat gaaaaaagta tctattgata gaagaactat acgtactaaa aaaatgatta
    27001 ggtctgcatt agcagagtta atagatgaga aaggatttaa caatatatca gttactgatt
    27061 taactcaaag ggcagatata aatagaggaa ccttctatct gcattatgtt gataagtatg
    27121 acttgttaga gaggattgaa aatgaaataa tacaggaaat tgaagaggag accaaacatc
    27181 ttgattcatc caacatgatg gagatagata cctcaagtga acctcttcca tttatggtaa
    27241 agctttttga atattttaga aaaaattcag taattattaa atctatcttg ggtcccaaag
    27301 gtgaccctac ttttgaaaga aaaataaaaa aatttattga aaccaatttg tttgagaaac
    27361 aaaaactaat aaattttaac atagatgagg caataatttc agaagaatat tttatacaat
    27421 atatactttc agcagactta ggtgtaattc aatattggct tgaaaaaaat atgaaagagt
    27481 cccctgaaga aatggcactg atacttgcta gaatgtcgtt actcggtccg ttaagagcag
    27541 tgggaattcg taaagattca taaaaatagt tttatatata ttagactttg aaaatatatg
    27601 tttaaaaacc attttttact actactgttt ataaattagg ggagatatct taaaatatta
    27661 gtaaaaggag tgctaagtga gtgtaataac tatgagataa gaggtttagt tgatatagtc
    27721 aaaacttcaa aagaatcaat tagaaaagtt aaaatattgc aattaaattt aaaggagtaa
    27781 tatatgaaaa atgaaaatat aagttatttg gcgttaggat tatgttttgg tgttgttttg
    27841 ggttcattat ttaaaaactt agctattggt ttatcctttg gattagttat tggagtagta
    27901 ttagacagta agaaaaataa ataaaacttc aatagtaaca tataacaatt atactatttt
    27961 tgtataaaat cattggaaat caacaataga aaatatttgt tatttaaaaa ctaaaaatac
    28021 agtttaaacg acctaaaaat atattatata attattataa caagataagt gagagtaagg
    28081 ttatgataat agagtaatat ttttcaatta ttactctatt tatatttatt tagttatact
    28141 atagtttata ttgttatttt attttcatat gcagtatagg gaatggatta ccttgttcat
    28201 cgaactcaga ttttttaaag actttaaatc ccatatgttt ataaaagcct aatgcttgtt
    28261 gattttgctc attcacatct acataattta catttaatac ttctattgca tattctacaa
    28321 gtctttttcc aattccattt cctatactat aattacttac aaataacatt tcaattttaa
    28381 aatcatgtat tcccataaaa gcttttataa tacctttttt tgtctcttac acataaaaaa
    28441 ttactaacat atttagcaca ttctttaact tgaggcttta ttgatattat attttcttca
    28501 cttaaaaagt catgtgttgc tcttactgat gattcccgtg tatctagtat ttctttcata
    28561 tctttattgt taataatatt taaattttca atttttatca tttaataatt cctcttaagt
    28621 aagtatgtta cttaatatta tatgttaaat atgatttaaa aaggttataa gaatattttt
    28681 gtattaaata taatcaaata aatatattta tttgtcataa attaaatgtc cactaaaata
    28741 gaaaaaataa gaagaaatag gtaataaatg atagcttata gcaaaataaa gatgtaagta
    28801 taacaataga tgagattgga attaagtcac tatacctaac agaacattct taggcgaata
    28861 tagaagactt aaaaaagaaa agagaagtag acaagaattt attaatgatt acatataaaa
    28921 taaatgaatt ataattttat taatgatata ataataatta acaaaaaata aaagaggaac
    28981 gagttaatgt ttgatggcat acactcattt tctaaccaat aaaacaatag attcaattaa
    29041 ggttactaaa gtctaatcaa cacataaaca tataggataa atacatcttt ttaatatatt
    29101 aattatgtta gacttacaaa aaatgtaaat acatagcttg ttaaatcttg attgaacctt
    29161 aagtaataag ggataaaaaa tgacttgttc aaaggcaaag aatatactat taaatgatat
    29221 aagtaactta gagataatat gtagtaagtc aaaagaacta tatagcatca gtaagtaaaa
    29281 agcgtaaaaa cttacataat gcactataaa ataaatgagt tagaaaataa atataattta
    29341 tataataaag tagtacaaaa aggagtattt atgtttggat atgttaaaat aaacaaaatg
    29401 gatttaacat ttagagaata tgattattac aaagcttact attgtggact atgtaaatac
    29461 ttaaaaagaa atcatggaga aatctctagg ttttctttga attatgatat aacattttta
    29521 attgttttat taacagcagt atataaccca gagtctataa gtacagagga agtgtgtata
    29581 gtaaatccat ttaaaaagaa aaaggtaatt acaaatgata ttacagaata tgcagcaagt
    29641 atgaatattc ttttaacgta ttataaattg gaagataatt taatggatga taaacgcata
    29701 aaagataaat tggcatatta tatatataaa aacaaattaa aattagcata tgaaaagtat
    29761 cctgaaaaag cagagtatat aaaacaacaa ctaaatgaat taaataagtt agaaaaagat
    29821 aaaaatataa atatagatga agtatctagt atatttggaa atataatggg tgaagtattt
    29881 gtctataaaa aagatgaaaa tgagagaaac ttaagaatga taggttttaa tataggtaag
    29941 tatatatatc tactagatgc ctatgaagat ttagatgaag attttaagaa aggaagatat
    30001 aatccattta tagagtatat tgataaaaat gatgagttaa aagaaaaagt aaaaaaaata
    30061 atagtaacat cattaggatt tttagctagg ggaattgata atctaaatct aaataaaaat
    30121 gttggaataa tagaaaatat aatttattct ggtgtttatt taagatatat taatatatta
    30181 gaaagtagag gtggaaagaa tgtacagtaa tacttataat aatgaaaatt ttatgagagc
    30241 aagagcattc atagaaaata gtgaatttaa aaaagcatat gattttttaa aaactttgac
    30301 tgacaaatgt gcagagtggt attatttaac tggcttttct gcaatgaaca taggatatta
    30361 tgaagaaggt gaagacttct taaaaagagc aaaatttatg gagcctgaaa atagtgaata
    30421 tagtgatgca cttagaagtt atacacaata tagaaatgat tatagcaata gggctgacaa
    30481 ttataataga agaagacgta atgatttaga tggatgttgt tgctgttgct gtgatgattg
    30541 ctgttgttgt ctaggtgacg attgttgtga aaattgtgct aagctttggt gtttagattc
    30601 atgttgtgaa tgttttggtg gagatttaat aacttgttgt taggatgata aatatgagta
    30661 taaaagttgc ttatggagga gttttattag cattaaatgt gatattgttg actcttacaa
    30721 atattattcc tgtaaataca ttgtttatta tggggttagc atcactttta gtatcaattg
    30781 taataatgga atggggcttt aagagtggaa tcgcttttta tataggttct atagttttag
    30841 gatttatagt gatggcaagt aagtctcaat ggattgtata ttcacttacc tttggaatat
    30901 atggtattgt aaaatatctt atagaaaaag ataggtctat atatattgag tattttatga
    30961 aattagtatt tgcaaatata atgatactta tattgtattt tttattaaga acaatagttt
    31021 atattccaat aaacatattt attataggtt catttgagat agcctttata gtatatgact
    31081 atgtttacag ttcttttata ggttattaca ataataggtt aagaaagatg ctatttaaaa
    31141 aataatagtt gatttttata tttaatttta atgaaattgt agtttggata agtgtaaatg
    31201 atatatctga atatacatta aagcataaag ctaaatttaa tatattctta gtgcaacata
    31261 taaaagcttt gatttttagt caaagctttt atatgttaga tataaatatt agatataata
    31321 taagttttta aaatagatag tataaattaa atatccacta tatttacatc tgtagataga
    31381 gaataaataa tatcttttct ttctactatt atctcaacat ttttagaaat ttcctcatca
    31441 agtgatattt gagataaaag ttcctttgtt ggatttattg ctattggatt tccaactctt
    31501 tttagcatat ttatatcacc attagtatct ccataggcat acgagtttga taaatctaaa
    31561 tcatacttta caacaaaatc atttatagca ttattcttac ttatagaatc ccacattgga
    31621 ataactgtgc ctgtaaatat attattttca aagacataat cacttcctag aaaatcggtt
    31681 acattatatt tttttgccat tttttcaaca agaaatccag ggctaccaga tataaatata
    31741 actatgtgtc cttgatttag atgccactca atccttgaac gtgtgtattt ataaactcta
    31801 tctgctttta atttaattac ttggtcactt gtaaagttga tacaggtttt atctaatcct
    31861 tttagtgaat caacataaag gtcacatatt tctaatagat aatcatcata attaccttgt
    31921 cttttatccc aatccataaa agtgtctcta gcatgtttat accaattttt ttggtcaatt
    31981 atatcgtatt taattaattt tttaaaatgt tcaaccataa gagagtctct ataaagagta
    32041 ccatctatat caaaaaaagc tgctatattt ttcataagac cacctcaatt aatcatttcc
    32101 ttatattata ttataaattg tatctaagtt gttaaaatat atgtataata atttatatat
    32161 taaaaaacaa gtattttaga gtttaaagga tgtgactata tgaattttaa ttttgcaaag
    32221 gctacaaatt ctagacttat gggcagttta ggtcttataa taaattggat agatgatgaa
    32281 aacaatcact tttgtcaata ttttttatta gatgcagagg gattaggatt agcagattat
    32341 gtgagtttaa ataacccaac acaagaagaa gcttatatgg aagaagaaag attgatggga
    32401 ggatttggtt cagatagagt tgaattaacg aaggatgaat ctttattttt agtatcttat
    32461 tttggtaata agaatcttta ttatgataag ctattgccag gagataaatg tgagtatatt
    32521 gatattataa aaaactataa aacagattta attatagaaa aattatacaa taaaatatgt
    32581 aaaagagttg atgaggaagt tgaatttata aactatatga ctatgagatt tatagcatgg
    32641 gatagagaaa gtttaaaata tttttctgga agtgacgaga ttgcaaatat gcatattact
    32701 aatataaatg gaacattact taaaaacgtg gtaagtgata aaggtcaagg cagatatata
    32761 agtgaagctt tatatgaaga caatgatgga tattatatat gtaaaattgc tttttgcata
    32821 agtaagtgct atgaaactgg ttttagaatt aattctctat tagtgacaga taaagaactt
    32881 atgtatgatt ttgaggtatt tgatgagatt tctaaaagtg aatttgtaag tgtttacagt
    32941 gtaaattcac ctgaagaatt tgcaggagta ttttatagag aaaatccatt tctgttaaaa
    33001 agtaatatga atgaaggtat attttttact agatttaatt ttaataatga ccatgtaaaa
    33061 gaaaatgtat atataataaa caatgatatg aaagctatat attacttaat gggaaataag
    33121 ttttttattg gtacttataa cgaaaatgat tgcaattata tcaatgaaat aagtcagtct
    33181 aattatagtg actatattaa atttgaagag aaatttttct ttgagcaaaa tgcattatat
    33241 gactttgcag aatcaggttc attagatttt gatgattttt tggaataata aatttagaca
    33301 ttatttaagc gagattaact gaatttaatc tcgcttaaat ttatttctat aatattgggg
    33361 gaatattatg aggaatacat ttattattgt ggtcaattat taaaatttta tacagtttat
    33421 tataaaaaat ttatactttt ctttatgttg tttgccaata ttagtctcaa atacgagtac
    33481 catactattc tgacccacca acacaagaaa gagtttttta aatgaaaaag tcagataaaa
    33541 ttaaatttta tctgactttt tctatgttta tcttattaga ttatgttaat atctgttgac
    33601 cgaataagcg catactcagt caactttcta tacctataat aatattgggg gaatattatg
    33661 aggaatacat ttattattat ggtcaattat taatgtttta tacaacttgc tcaaaaaaat
    33721 atatattaca tttcttcctc atcaaattct tcaatattag tagcctgtgg tataccttgt
    33781 atagacatat tactagaacc ataaggagta ggatatatat taggaggcat agtactaggc
    33841 ataactacag gcataccttg cattgtatta tgacatataa ctcctggtat catcataagt
    33901 acattaggag gcatattttg attacatgag aagttaggaa tacccatagt attattcata
    33961 ttatacccca tattactgtt catgttatta ggcacattgt taggcatacc catgttacta
    34021 ttcatattat taggcatatt gttaggcata cccatgttac tattcatatt attaggcata
    34081 ttgttaggca tacccatgtt actattcatg ttattaggca tattgttagg catacccata
    34141 ttactattca tattgttaga catattgtta ggcataccca tgttactatt catgttatta
    34201 ggcatattgt taggcatacc catattacta ttcatattgt tagacatatt attaggcata
    34261 cccgtattat tattcatgtt attaggcata ttatttgtag cagatgtatt gtaattcata
    34321 tttccggatg tattatttgt gttaggcaag ttgtttggag aattactact tttaggataa
    34381 tgatttacat tattaggata taggttattt atgttctggt tattaggtat ttttgtataa
    34441 ccattagaaa tttgagggtc atttctcaat tcatcttggg gaatttcttc attagtaata
    34501 taatctgcat agttcgctgg gtctaagtaa ggcatgtaac tagtatctaa atcctttttt
    34561 ttcttgttac tcatattaaa cttgcacctc ctagaaaact ataaaaaagt ttacaaaaac
    34621 ttattctaat aatatatata ttaaaaaagt ttataatatg tgcattctaa ttatattaaa
    34681 ttaataaaaa ttattgttgc aaatttagga atgatagcat ataatgaaca tatgaacagt
    34741 tattcatata ttaaaacaat ggaggattaa agatgagaga agagataaat gattgtaatt
    34801 gtaatatagt acatgaagaa atagtaacag aggctaaatc aactatgcca gatgaagaga
    34861 tgttatatga tttagcagaa ttatttaaag tatttgggga tactactaga gtaaagatat
    34921 tatatgcatt atttgcaaat gaaatgtgtg tatgtgatat agcgagttta ttaaatatga
    34981 ctcactctgc aatatctcat caacttagag tactaaagca ggctagatta gtaaagttta
    35041 gaagagaagg aaaaacagtg tattactcat tagatgatag tcatataagc cagatatttg
    35101 attgtggatt aaatcatatt agagaaactt ataaatgata aatgagagga gtgaatatat
    35161 atggaagcaa ataatttaat taaaaaagaa tttatactag gaggattaaa ctgtgcacat
    35221 tgtgcagagg agattaataa taaagtttct aaactacaag aagttaaatc ttccaactta
    35281 aattttataa ataaaaaact tatagtaaat ataaaggaaa gctttaatga ggatactact
    35341 atagaaaaga taatagatat aatagattca acagaaccag gtcttgatat acaaataagt
    35401 tctaaagaaa atgcatctag taaaacatca attaaaaaag agcttatatt aggaggatta
    35461 aactgtgcac attgtgcaga ggagattaat aacaaggttt ctaaattaaa agaagttgaa
    35521 tcttctaatt taaactttgt aaataaaaaa cttacagtaa atataagtaa taactttaaa
    35581 gaggatgatg ttataaataa aataaaagaa ataataaatt caacagagcc aggacttgat
    35641 atacaagtag ggtctactga caaagtaaaa gggagaacta ctgaaaaatc aggagctgta
    35701 aatgatacaa ataagaaaga attagttcca cttataattg gagcacttgt atatatattt
    35761 ggtatatatc aaacagctac aggttatgaa agtcagttta gcaatatagt atttatagtt
    35821 gcctatgtaa tagtaggtgg cgatgtgctt ttaagagcaa taagaaatat atcaaaagga
    35881 agagtatttg atgagaactt tttgatggct ctagcaacag taggagcatt aacaataggg
    35941 gagtcatcag aagcagtagg ggttatgctt ttctataaag taggagagta tttacaaggt
    36001 gttgcagttg gtaaatccag aaagtctata acttcactta tgcaaataag accagattat
    36061 gcaaatctaa aatttaattc tgaagttaag gtagtgtcac cagaagaagt aaatgtaggg
    36121 gatataatag tagtaaaacc tggtgaaaaa gtaccattag atggtgtagt agtggatgga
    36181 gtttctatgc tagatacttc tgcactcaca ggagagtcag tgctgagaga agttgaaaaa
    36241 ggagatgaaa tcttatcagg tgttataaat aaaaatgctt tattgagtat tgaagttaca
    36301 aagagttttg gagaatcaac agtttctaag attcttgacc ttgttgaaaa ttctagcatt
    36361 aaaaaatcaa agacagaaaa ctttatatct aagttttcaa gatactatac tccaatagta
    36421 gtaatagctg ctttattaat agcatttgtg ccaccacttg taatctcagg tgaggtattt
    36481 agtgattggt tatatagagg attgatattc ttagtcgttt cttgcccttg tgcattagtt
    36541 ttatcaatac cacttagttt ctttagtgga attgggtttg catctaaaaa tggtatcctt
    36601 attaaaggaa gtaactattt agaagcttta agaagtgtag atacagtagt atttgataaa
    36661 acaggaacac ttactaaagg tgtgttcaat gtaactaagt taaatcctga aggtatatca
    36721 gaagaagaat tattagaata tgcagctatt gcggaggtaa attcaaatca tccaatagca
    36781 aagtctatat taagttacta taataaaaaa attgatttag atacaataga cagttatgag
    36841 gaaatagcag cttatggaat tagagtaaag cataatggca attttatatt agcaggtaat
    36901 gagaaactta tgaaaaaaga aaatatatct tattcatcag ctaaagaagt aggcacagta
    36961 gtctatattg ctgttgataa agtatataga ggttatatag ttatatctga tgaagtaaaa
    37021 gaagatagta aaaatgccat aagaagctta aaagcaatag gggtaaaaga agttgtaatg
    37081 ttaactggag ataatgagaa agtagcaaaa aatattgctc aagaattaga attggataca
    37141 gtttactcaa atttacttcc taatgaaaag gtagatagat tagaagattt atatgaagga
    37201 agaactgaaa aagagaaaat agcatttgta ggtgatggaa taaatgatgc tccagtatta
    37261 gctcgtgctg atgttggtat agctatggga ggcttaggtt cagatgctgc tattgaagct
    37321 gcagatgtag tgcttatgac agatgagcct agcaagatat ctaaagcaat tgaaatagca
    37381 aacaagacta ataagatagt gtggcaaaac ataatatttg ctctaggagt aaaaattata
    37441 gttatgatac taggtgctgg aggagtagct actatgtggg aagctatatt tgctgatgta
    37501 ggtgtggcac ttatagcagt agtaaatgct atgagagcta tgagataagg gataaatgta
    37561 aatccatact atttaaaaaa cttataatta tatattgaag tatgtaataa tgaaaggtat
    37621 caataagttt cttttaactt tattggtacc tttttattgg agcgctttgc atatataaat
    37681 ttatattcat tatccattgt gtttatttaa tgtatttatt tagtgtagag cttttatatt
    37741 gtaattttaa aatttagaca tagaaggttt attaataagt ctatattaag tttaaattag
    37801 attcataaaa attataatac ttatgattgt atatatatta tattaatagt tataattttt
    37861 acaacaaaat gtgtgtgtta aaatactcta aaccaacagt atgactgtca ttcaataaat
    37921 tatgtaatat aagaaagtaa atatttttaa aaaataattt aaaaaagtat tgacgaaata
    37981 tttttaag
//
LOCUS       DAMJVX010000019        37805 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMJVX010000019 DAMJVX010000000
VERSION     DAMJVX010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.020"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            152..328
                     /locus_tag="PC407_000931"
     CDS             152..328
                     /locus_tag="PC407_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496391.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     SILEKELNISKANT"
     gene            502..1632
                     /locus_tag="PC407_000932"
     CDS             502..1632
                     /locus_tag="PC407_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2496392.1"
                     /translation="MNNFEIKTRIKFGEGSLKVLQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKSIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILNNIK"
     gene            1873..2223
                     /locus_tag="PC407_000933"
     CDS             1873..2223
                     /locus_tag="PC407_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2496393.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKRD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            2231..2680
                     /locus_tag="PC407_000934"
     CDS             2231..2680
                     /locus_tag="PC407_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HDF2496394.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDDIGLEKLFKYLEKSKNIDEV"
     gene            2782..3357
                     /locus_tag="PC407_000935"
     CDS             2782..3357
                     /locus_tag="PC407_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2496395.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            3350..4759
                     /locus_tag="PC407_000936"
     CDS             3350..4759
                     /locus_tag="PC407_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HDF2496396.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSNENGTEVLITIPTQINI"
     gene            4943..6376
                     /gene="eutA"
                     /locus_tag="PC407_000937"
     CDS             4943..6376
                     /gene="eutA"
                     /locus_tag="PC407_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDF2496397.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQTIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSVDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            6394..7758
                     /locus_tag="PC407_000938"
     CDS             6394..7758
                     /locus_tag="PC407_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HDF2496398.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFYKDGKLTELAGDAS
                     VLLG"
     gene            7771..8652
                     /gene="eutC"
                     /locus_tag="PC407_000939"
     CDS             7771..8652
                     /gene="eutC"
                     /locus_tag="PC407_000939"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HDF2496399.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKTTEVVVDNNAFI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSQETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            8672..9325
                     /gene="eutL"
                     /locus_tag="PC407_000940"
     CDS             8672..9325
                     /gene="eutL"
                     /locus_tag="PC407_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDF2496400.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEIAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            9336..10037
                     /locus_tag="PC407_000941"
     CDS             9336..10037
                     /locus_tag="PC407_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2496401.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRIDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEITDNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            10040..11509
                     /locus_tag="PC407_000942"
     CDS             10040..11509
                     /locus_tag="PC407_000942"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HDF2496402.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNIPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            11605..11892
                     /gene="eutM"
                     /locus_tag="PC407_000943"
     CDS             11605..11892
                     /gene="eutM"
                     /locus_tag="PC407_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDF2496403.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            12019..12780
                     /locus_tag="PC407_000944"
     CDS             12019..12780
                     /locus_tag="PC407_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HDF2496404.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLEDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            12793..13422
                     /locus_tag="PC407_000945"
     CDS             12793..13422
                     /locus_tag="PC407_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2496405.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            13464..14189
                     /locus_tag="PC407_000946"
     CDS             13464..14189
                     /locus_tag="PC407_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HDF2496406.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEILEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            14202..14474
                     /locus_tag="PC407_000947"
     CDS             14202..14474
                     /locus_tag="PC407_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HDF2496407.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            14467..15018
                     /locus_tag="PC407_000948"
     CDS             14467..15018
                     /locus_tag="PC407_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2496408.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYVVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            15030..16121
                     /gene="eutH"
                     /locus_tag="PC407_000949"
     CDS             15030..16121
                     /gene="eutH"
                     /locus_tag="PC407_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2496409.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NSGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLRKENV"
     gene            16123..16596
                     /locus_tag="PC407_000950"
     CDS             16123..16596
                     /locus_tag="PC407_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HDF2496410.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(16716..16817)
                     /locus_tag="PC407_000951"
                     /pseudo
     CDS             complement(16716..16817)
                     /locus_tag="PC407_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            17112..17633
                     /locus_tag="PC407_000952"
     CDS             17112..17633
                     /locus_tag="PC407_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2496411.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            18012..19187
                     /locus_tag="PC407_000953"
     CDS             18012..19187
                     /locus_tag="PC407_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2496412.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQAYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            19222..20760
                     /locus_tag="PC407_000954"
     CDS             19222..20760
                     /locus_tag="PC407_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2496413.1"
                     /translation="MQGALKENKKKSGGGFLGWIERVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQVI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            20792..21994
                     /locus_tag="PC407_000955"
     CDS             20792..21994
                     /locus_tag="PC407_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2496414.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFIQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     SEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     IWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(22173..22772)
                     /locus_tag="PC407_000956"
     CDS             complement(22173..22772)
                     /locus_tag="PC407_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2496415.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            22941..24299
                     /locus_tag="PC407_000957"
     CDS             22941..24299
                     /locus_tag="PC407_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496416.1"
                     /translation="MSNIITNLIESVFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            24292..24924
                     /locus_tag="PC407_000958"
     CDS             24292..24924
                     /locus_tag="PC407_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2496417.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSKIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            25253..26410
                     /locus_tag="PC407_000959"
     CDS             25253..26410
                     /locus_tag="PC407_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2496418.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     FNSEEIVYFTPKGKSYHSIKNCSGLSRSKKILSGTIEESKKNDPCDICYGK"
     gene            complement(26558..27193)
                     /gene="lexA"
                     /locus_tag="PC407_000960"
     CDS             complement(26558..27193)
                     /gene="lexA"
                     /locus_tag="PC407_000960"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDF2496419.1"
                     /translation="MYLDLTEKQVLILEFIKSQITLKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(27297..28424)
                     /locus_tag="PC407_000961"
     CDS             complement(27297..28424)
                     /locus_tag="PC407_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2496420.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(28746..29591)
                     /locus_tag="PC407_000962"
     CDS             complement(28746..29591)
                     /locus_tag="PC407_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496421.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(29621..29992)
                     /locus_tag="PC407_000963"
     CDS             complement(29621..29992)
                     /locus_tag="PC407_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2496422.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIEDACKILSCNGYEVE"
     gene            complement(30145..30405)
                     /locus_tag="PC407_000964"
     CDS             complement(30145..30405)
                     /locus_tag="PC407_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HDF2496423.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(30594..31538)
                     /locus_tag="PC407_000965"
     CDS             complement(30594..31538)
                     /locus_tag="PC407_000965"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDF2496424.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(31559..32413)
                     /locus_tag="PC407_000966"
     CDS             complement(31559..32413)
                     /locus_tag="PC407_000966"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDF2496425.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKELLY
                     QILAMHKK"
     gene            complement(32428..33789)
                     /gene="accC"
                     /locus_tag="PC407_000967"
     CDS             complement(32428..33789)
                     /gene="accC"
                     /locus_tag="PC407_000967"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDF2496426.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(33805..34257)
                     /gene="accB"
                     /locus_tag="PC407_000968"
     CDS             complement(33805..34257)
                     /gene="accB"
                     /locus_tag="PC407_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDF2496427.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(34527..34973)
                     /locus_tag="PC407_000969"
     CDS             complement(34527..34973)
                     /locus_tag="PC407_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496428.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            35248..35526
                     /locus_tag="PC407_000970"
     CDS             35248..35526
                     /locus_tag="PC407_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496429.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(35655..36401)
                     /locus_tag="PC407_000971"
     CDS             complement(35655..36401)
                     /locus_tag="PC407_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496430.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLDFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(36530..37174)
                     /gene="trmB"
                     /locus_tag="PC407_000972"
     CDS             complement(36530..37174)
                     /gene="trmB"
                     /locus_tag="PC407_000972"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDF2496431.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(37312..37632)
                     /locus_tag="PC407_000973"
     CDS             complement(37312..37632)
                     /locus_tag="PC407_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HDF2496432.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
BASE COUNT        14474 a         4870 c         5995 g        12466 t
ORIGIN      
        1 ttgatattta attgtatcag gacaaatctc taatttcata tattattaat ataaaatcat
       61 aggttagtgt gaaaaagttg aaatttaaat attttaaaag ataatttttt ggaactaata
      121 ggtataacaa aaatttatag ggggattgca tatggaaatc aatgttatag aaattttccc
      181 taaagataaa gctaaactta ataaaataga aatggataaa gctagttggt ttgtaaatat
      241 aataagtaaa aaatatccta aagaagcttt aaatgaagca tttagtattt tagaaaaaga
      301 attaaatata agtaaagcta atacataagc tttgattatt gattttatat actattggtt
      361 ttatacgcta tgacttgaca ttatattaat caaatagtat atttttaaat atagcacaat
      421 gaagtgttaa aagagcaatg gagctccata gataaagtct atggagcttt tttgcgtatt
      481 taacttaagg aggatagagc tttgaataac tttgagatta aaactagaat aaaatttgga
      541 gaaggttcac taaaagtttt gcaagaatta aaaaacaaaa atgtattaat aataacagac
      601 ccttttatgg tgaaatcaaa aacaatagat aaaataacat ctaatttaat aaactcaaca
      661 tatcaagttt ttgaagaaat agtaccagac ccaccaatag aagtagtagt agcaggaata
      721 gaagcattta aatctataaa tccagacgta ataatagcat taggtggtgg ttctgcaata
      781 gatgcagcaa aatctataat ggacttttct aaaaaaatat taaaaattaa cgaagtagag
      841 tttatagcaa taccaacaac aagtggaaca ggttcagaag taacatcatt tgctgtaata
      901 acagatagtc aaaaaaaatg taagtatcca ttagtgagtg atgatttact tccagatatg
      961 gcaatactag acccagagct tgtaaaaact gtaccaaatt ttataacagc agatacaggg
     1021 atggatgtat tgacacatgc tatagaagct tacgtatcta caaattctaa tgacttttca
     1081 gatgcactag ctgaaaaatc tataaagtta gtatttaaat acttaataaa agcatataaa
     1141 gatggaaatg acatagaagc tcgagaaaaa atgcacaatg catcttgttt agcaggtatg
     1201 gcatttaacc aagcatcact tggcataaat cattctattg cacatgtatt aggtggaaaa
     1261 ttccatatac cacatggaag aacaaattct atattacttc ctcatgtaat agaatataat
     1321 tcatcaatag taggatatac taacacaact tactctgata cagctaaaaa atatgcacaa
     1381 atagcagaat tagtaggact tgggagtgct aatataagaa tatctgttag aaatttagta
     1441 aatgaaataa agaaactaca aaaagaaatg aatatgccaa ctaaattgac acaatgcaaa
     1501 ttaagtttag aagatataac taatgaagaa agagaaatag caaaacttgc tataaaagat
     1561 acttgtacat taactaatcc taaggtacca actgaagatg atataataaa catattaaat
     1621 aacattaaat aactacattt tattacatgt aaatataata ttatacaaat agggaaaatg
     1681 tttttaaaaa tataaattat atattgacaa atataaaaca taaaactata atgaaagtat
     1741 cacaaaggcg tgatacttta gtagaaagca atggagcttc ttaggaatat taatattatt
     1801 cttatggagc tccttttatt ttaagcaaat agctttttaa taaactatat tcaaagaaag
     1861 cagggaataa atttgactga agaatctaag caaagagtta ttcaagaata tgttcctgga
     1921 aaacaggtta cattagcaca tatcatagct aaccctaatg aagacatata taagaagtta
     1981 ggattagtac ttgataaaag agatgcaata ggaatactta caataacacc aagtgaagcg
     2041 tcaataatag cagcagatgt tgctacaaag gcttcaaatg tatctttagg ttttatagat
     2101 agatttagtg gttcagtagt aatatcaggt gatgtaagtt ctgtagaatc agcgttaaat
     2161 gatgttttag aagtcttagg aaatatgtta aacttctcat ctacaaaaat cacaaggaca
     2221 taataataca atgaagaata taatatttat gggaaagaca ggtagtggta aaactacctt
     2281 atgtcaaaaa ctagaccagt tagaattaaa gtataaaaaa actcaatcag tggaacttta
     2341 taataatgca atagatactc ctggtgagta tatggaaaat agaaacctat ataatgcctt
     2401 aataacaaca tcagcagatg ctaaaattat agcaatagtg tatgatgcca cacaagaaga
     2461 aaattatata gcaccaggtt ttgccagtat gttttgcaaa gacgtaatag gaattataac
     2521 taaaataaat aaagctactg aaaacgaagt caatatagct tatgaaagac tatcactagc
     2581 aggagcaagt caaatcttta aaatagacac agttgatgat attggacttg aaaaattatt
     2641 taaatatcta gaaaaatcta aaaatataga tgaagtttaa ggggaaatat ttttttattc
     2701 aaaaatatta ttaaagtatg ataaaataaa aaaatgtata aactatttta agaagattta
     2761 atctttaagc gaggtaggaa catggggaaa aaaatactat tggttgatga tgaaccactg
     2821 ttaaggatgg atacaaaaga tatattagaa agtcatggct atgaaataat aggcgaagca
     2881 agtgatggat ttgaagctgt tgaaatgtgt aaaaaacaca aacctgattt agttataatg
     2941 gatatagaga tgccaatact tgatggtata aaggcaggca aaataatatg taaagaaaat
     3001 ctagctggtg gagtactttt attaacttca tttgaagata aagaatacgt agaaaaggct
     3061 aaaagtatag gtgcttatgg atatttagta aaaacagcaa gtgaaaaatc acttatccca
     3121 accatagaaa tgtgtttaaa taaggtaaat gaatttgaaa aaatgaaaaa agatttagaa
     3181 aaagtaaatt ctaaattgaa tgaaagaaaa ttagtagaaa gagcaaaagg tatacttata
     3241 agagagttta agatttctga ggatgaagca tacactagaa taagaaaatt aagtatggat
     3301 aaaagaacta caatgatgga aattgccaaa atgattataa taggatacga tgactaatac
     3361 gataaaaaaa atatcaactg cacaaacata cttaaataat aacgaaattg aaaaaattat
     3421 agaagtatca aaatccttag atttaatggc ttctttttat gaggctgatg tttttataga
     3481 tgtactaagt aaatttgatg atgaagcaat tgttgtggca catggtagac ctaaagaaaa
     3541 gtcaatgtac tgtgaaaatg ttataggtaa aaaggcttta attgaaaatg aaccaggagt
     3601 tataaagtgc ttaaaaacag gagaaagagt aagagatatt aaagctttaa cacaggaata
     3661 taaactggtc aaacaaaata ttcagccaat aacactagat gacaaagttt tagcagtact
     3721 aatagttgaa aaagatataa gtagtgaagt taagaatgag tttaatgcta gtgaagacga
     3781 agaaaataaa aatttacatc agtttataaa acttatcgga aataatgact ttttagttga
     3841 taatttaaat tcagccatat taatatttga taaaaatgga aaattaaaaa ttaaaaatcg
     3901 tattgcaact gagatataca aaaatcttgg gtttggatat gatatacaag atatgcatta
     3961 caatgattta gatatagaaa ccaacacctt tgaaaatata ttaatggatg aaaattatga
     4021 tgagacaaat gaagtttcaa taggtaatat gttttttaaa ataaagacca tatacatcaa
     4081 agatgatgaa tttaaggttt gcaaaatagt acaagatata agtgatttaa agagaaaaga
     4141 agaagaatta gttttaaaaa cagtggctat aagagaagct catcatagag taaaaaataa
     4201 tcttcataca gtaatatcta taataaaaaa acaaagtaga ttatctcaaa atgatgaagt
     4261 aaagcagtgt ttagataaca taataaacag agtatttgca atactttcaa ctcattattt
     4321 actatctaaa gaagttgata ataatatatc tatacgagaa ggtatcaatt tattagtgtc
     4381 aaatattcaa ggtggctatt tatttagtaa ggatatagat atacgtataa ctggtgaaga
     4441 ttttaaaatt agtggagaaa aagcaacagc aatacttttg gttataaatg aaatactaca
     4501 aaattgttat gaccatgcat tttcggatag agaacatggt actatacaaa tattaataaa
     4561 taaagataac gactatagaa atattgctat tatagatgat ggaattggat ttgatagtaa
     4621 taatataaat aaaaatagct tagggacata tataatagat agttatataa cacagatgtt
     4681 aaaaggaaat atagagagaa aaagtaatga gaatggtaca gaagtattaa ttactatacc
     4741 tacacaaatt aatatttaaa taataactca tctacaaagt agtagtttta attgagcaaa
     4801 gaggcttaag gttattaata gattaagtta attgaatgta tttataaaaa ttataagtat
     4861 atattcaatt taacaggtat ttattataac tttaagcctc tttgcttgtt taagacatac
     4921 ttaaatttta ggaggtgaga aaatgagaga agagttatta agtgttggta ttgacattgg
     4981 aacaagtact actcagttaa ttttttctaa attaatagta gaaaatactg aatcaaattt
     5041 ttcggtacct agaataagta tagtagacaa agaaattatt tataaaagca aagtttattt
     5101 cacccctctt atttcaaata atgaaataaa tggtcaacaa ataaaagata tagttgaaaa
     5161 tgaatataaa aaagcaaata tatctaaaga ggatatacat acaggagcag taataataac
     5221 tggtgaaaca gctagaaaag aaaatgcaaa tgatgtactt catactttga gtggatttgc
     5281 tggggacttc gttgttgcaa cagcaggacc tgatttagaa agtataatat caggaaaagg
     5341 agctggagct catatttatt caaaagaaca ttcaactaca attgtaaata tagacatagg
     5401 tgggggaact agtaacatag cctcatttaa aaggggagaa gtaaccgata ctgggtgttt
     5461 ggatataggt ggaagacttg tgaagataga taaaataagt aaaaaaataa cttatatatc
     5521 acctaaaata gataaaatca taaaagataa taatttaaat cttcaagtag gcacaatggc
     5581 tactgtagaa aatacaaagc caatagtgga tataatggtt gacttattaa tggaaagcat
     5641 aggattaaag aaacagacac ctttgtttga ttttataata accaataaat caataagtat
     5701 agatgaaaat attaaaaatg tttcattctc tggtggagta gctgattata tttactatga
     5761 tggagaaata aaagattatt ttgaatatgg tgacttggga attttacttg ggcaaacaat
     5821 aaaaaattgt gaatacctta aaaaagtaaa tgtagttaaa tctatggaaa caataagagc
     5881 cacagtggtt ggggcaggtt ctcatactac tgaagtaagt ggaagtacaa taacttacac
     5941 tagcgaaaag tttccactta agaacttgcc agttttaaaa ttatcagttg atgaagaaac
     6001 aggtacttca aaacaaatca gtgaagctat aaaaaataaa cttaattggt ttaaactaga
     6061 aaatgatttg cagaaagttg ctatagccat gataggaaag aaaaatccaa cctttttaga
     6121 tatacaagag tatgctaaag ggtttgtaga tggaatgcaa caaatcatag aaaatgattt
     6181 accttttata gtaattgttg aaaatgatat ggcaaaagtg ttaggtcaag caatatatgc
     6241 attattaaat tatgaaaaag aaattgtttg tatagacagt gtcaaggttg aaaatggaga
     6301 ctatatcgac attggaaaac ctatagttga aggtaaagtt ttacctgtgg taataaaaac
     6361 attagttttt aattaataga aggaggtaag taaatgattt taaatacaaa attatttgga
     6421 cgtacttatc agtttaaagg cttatatgat gtaatggcta aagctaatga agaaaaatca
     6481 ggagataaat tagcaggtct tgcagcagaa tcagctgaag aaagaatagc agcaaaagtt
     6541 gttttatcac acataaaatt ggaggatata agaaataatc ctgcagtggc atatgaagaa
     6601 gatgaagtaa caagaataat acaagatgct gtaaatgaaa aagtttatga agaaataaaa
     6661 aattggacag tatctgaact tagagaatgg ctattggatt caaatactac aagtcttgat
     6721 ataagaagaa tatcaagagg attaacttct gaaatgatag cagcagttgc aaaattaatg
     6781 tcaaatatgg acttagttta tgcagctaaa aagataaagg ttactgctca ctgtaataca
     6841 actataggag aacaaggaac attatcagca agacttcaac ctaatcatcc aacagacgac
     6901 ccagatggaa tacttgcatc acttcttgaa ggtttaactt ttggtatagg agatgcagta
     6961 cttggattaa atccagttga tgactctgtt gagagcgtta ctaaagtatt aaaaagattt
     7021 gaagaagtaa aacaaaaata tcagatacca actcaaacat gtgtattggc acacgttact
     7081 acacaaatgg aagcaataag aaatggtgct cctgcagatt taatattcca atctatagca
     7141 ggttctgaaa aaggaaatga agcatttgga tttaatggcg acactataga agaagctaga
     7201 cagttagctt taaaacacgg tacagctaca ggacctaacg ttatgtattt tgaaactgga
     7261 caaggttcag aattatcttc agaagcacat catggaatag accaagttac tatggaagct
     7321 agatgctatg gatttgctaa aaagtatgaa ccattcttag taaatacagt tgttgggttt
     7381 ataggacctg agtacttata tgatgctaga gaagttgtaa gagcaggact tgaagaccac
     7441 tttatgggta aactacatgg cttaccaatg ggtgttgatg tttgttacac taaccatatg
     7501 aaggctgacc aaaatgatat ggaaaactta gccatactat taacaacagc aggatgtacg
     7561 tattttatgg gaatacctca tggagatgat gttatgctta actatcaaac aacaggatac
     7621 catgaaacag catcacttag agaaatgttt ggactaacag caataaaaga atttgatgct
     7681 tggatggaaa agatgggatt ctataaagat ggtaagttaa cagagttggc gggagacgca
     7741 tctgtattat taggatagga ggtatttaat atgaatgaga aggatttaaa agcattggta
     7801 gaacaattag ttggacaaat ggttggagaa ttagatacta atgttgtatc tgagactgtt
     7861 aaaaagacaa ctgaagttgt agtagacaac aatgctttta tagatgatat aacagaagtt
     7921 gatataagaa agcaattact agtaaaaaat cctaaggatg cagaagctta tttagatatg
     7981 aaagcaaaaa cacctgcaag attaggtata ggaagagctg gtactagata taaaacagaa
     8041 acagttttaa gatttagagc agaccatgct gctgcacaag atgctgtatt ctcttatgta
     8101 gatgaagaat ttataaaaga aaataatatg tttgcagtgg aaactttatg taaagataaa
     8161 gatgaatact taacaagacc agatttggga agaaaatttt ctcaagaaac tataaataat
     8221 ataaaatcta aatttggtac aaatcaaaag gttttaatat tagttggaga tgggcttagc
     8281 tcagctgcaa tagaggcaaa tttaaaagac tgtgtgccag ctataaaaca gggtctaaaa
     8341 atgtatggta tagattcaag tgaaatttta tttgttaaac attgtagagt tggtgctatg
     8401 gaccacttag gtgaagaatt aggttgtgaa gttatatgta tgttggttgg agaaagacca
     8461 ggattagtta ctgctgaatc aatgtcagca tatatagctt ataagccata tataggaatg
     8521 gcagaagcaa aaagaacagt tatatcaaat attcacaaag gtggaacaac tgcagttgaa
     8581 gcaggtgctc atatagctga acttataaaa actatgttag ataaaaaagc ttcaggaata
     8641 gaccttaaat aaagtcgagg gagtgtagaa tatgaaaaat gatgtaattc gtcctaatat
     8701 actaggaatt aaaataattt cgaatataag tccagaaatg gcacaaaaac tagaattaaa
     8761 atcacatcac aaaagtttag gtttaataac tgcagactgt gatgatgtta cgtatacagc
     8821 tcttgacgaa gcaactaaag cttcagaggt tgatgttgta tatgcaaaat caatgtatgc
     8881 aggtgctgca aatgcttcaa caaaacttgc tggtgaagta ttaggaataa tagcaggccc
     8941 aagcccagca gaagtaagaa gtggtctaaa tgcagtagtt gattttctag agtatggagc
     9001 aacttttata agtgcaaatg atgatgattc aatagcatac tatgctcact gcgtatcaag
     9061 aacaggtaca tacctttcag agatagcagg aataagagaa ggtgaagcat tagcttattt
     9121 agtagcacca ccacttgaag caatgtatgc actagatgca gcaatgaaag ctgcagatgt
     9181 taagatgtgt gaattatttg cacctccaac tgaaacaaac tttggtggag cattacttac
     9241 aggttctcaa tcggcttgta aagcagcatg tgatgcattt gcagaagcag taaaatcagt
     9301 tgcagacaat ccaacaggat tttaaggata gttagatgga tgctataggc ttaatagaag
     9361 taataggata tgttgctgca atagaagctt ctgatgcttg tttaaaatct gcaaatgtta
     9421 atattgtaag aattgataag gttggggcag gtattgttac tttaacaata tgtggagatg
     9481 tcggtgctgt aaaagctgct ttagaagcag gtgaaatagc agcttccaga gttggtacat
     9541 taagaacatc acatattata ccgagaatcc ataatgaagt aacaaatgct ttattcaaaa
     9601 ctaaagaacc taaagtctgt gaagtttcag aagataaaga aaaaaacttt gagattacat
     9661 tggaaataac tgataatgta tcgaaaaatt tatcagaaaa agaaaatgta attttagatg
     9721 tcaatgaaaa cagtggagat actgaaaatt tatatagtga tatagataaa acaaacaaaa
     9781 aaattgaaac agttgatgat agtttaacag aagctaatga ggaaaaaata gaaaatacta
     9841 atgataaaga agtattggaa caagttaaaa tagaaaattc cgattttgat agagatgacc
     9901 tatcaaagaa cagtgtaaaa aaacttaaag ctatggctaa gaaattagat tcttcaataa
     9961 cttataaaga tttaaatgtt cttaaaaaag aagaattaat aaacttagtt aaaaagctta
    10021 gcagaaggga tagatagtta tgacactttt agataaagat ttagtatcta tacaagaagt
    10081 cagaactctt gtaagaagat ctaaagtggc tcaagagaaa ctttctaaaa tgagtcaaga
    10141 gcaaatagat aaaatagtaa aagctgttgt agatgcagca tttaataatg ctactaaatt
    10201 agctaaaatg gcaaatgaag aaactggatt tggaaaatgg gaagataaag taattaaaaa
    10261 tacttttgca tctatgggag tttatgaaac tttaaagaac acaaaaacta ttggaataat
    10321 tggtgaagat aaagaagcca atacgataga tatagctaca ccagttggag ttgtggcagg
    10381 tcttatacct tcaacaaacc caacatcaac agtaatatac aaaactataa tatctctaaa
    10441 agcaggaaat actatagttt tctctccaca tccaaatgca aaaaactgca tacttgaaac
    10501 agttaatata ataagtgagg ctgctataaa ggctggagct ccagaggatt caatattatg
    10561 tataactaat ccaagtatag ttggaaccaa tgaattgatg aaacataaag atacaaactt
    10621 aatactagca actggaggag aagcaatggt tagagctgct tactcttcag gaacaccagc
    10681 tattggagtt ggaccaggaa atggaccagc atttatagat aaatcagcaa atgttaaatt
    10741 agcaatcaaa agaataatag attctaaaac ttttgataat ggtgttatat gtgcatcaga
    10801 gcaatctata gtaatagaaa aatctatgga agaaactgtt ttaaaagagc ttaaaaatca
    10861 aggtgcatat gttttaaatg aagaggaaag tgaaagatta gcaaaattca tattaagagc
    10921 taatggtact atgaatccac aaatagttgg taaaagtgtt gaggtaatag caaatttagc
    10981 agacttaaca aatataccta aaaatacaaa agttctagtt gcaagagaaa ataaagttgg
    11041 tcacaatgta gcctattcaa gagaaaaact tacaccaata ctagcgtttt atgttgaaga
    11101 aaatgtagaa gctattatga acagatgtag agaaatattg ttaaatgaag gtgcaggtca
    11161 tacattctca atgcatgtaa atgatgaaga attggtaaaa agatttgccc ttaatatgcc
    11221 agtatcaaga atacttgtga atactccttc agcacttgga ggaataggtg catcaacaaa
    11281 ccttgtacca gcactaacac ttggttgtgg tgcaataggt ggaagttcaa cttcaaacaa
    11341 tataggacca cttgatttaa taaatgttaa aagagtagct tatggaatca aagaaataga
    11401 agaacttgga agagataaat ctcaagttga aaaatcaatg tttgactcaa tggataaaga
    11461 agaattaata aatcttcttg ttcaaaaact agtcaaagaa ctagcataac taaaataaac
    11521 aatgaaactt atggctatcg ctataaattt ataaaaatat aaactataaa aaactataaa
    11581 attaaatttt tggaggataa gattatggca agtgcaaacg cattaggaat gatagaaact
    11641 aaaggattag taggagcaat agaagcagca gatgcaatgg ttaaagctgc aaatgtacaa
    11701 ttagtaggaa aagagcaagt aggtggagga ttagttactg taatggtaag aggagatgtt
    11761 ggagctgtta aagctgctac agatgcagga gctgcagctg ccgagagagt tggagaacta
    11821 atatcagttc atgttatacc aagaccacac tttgaagtag atgctatatt accaaaggta
    11881 tcagctgagt aatataagaa taattttaaa atttaaattt tgattgaaaa agaggctatc
    11941 tcaaaaatga gtcttactaa taaactcaat gtattttgag gtagtctttt ttatacatct
    12001 aataggagat gtgataaaat gggtgtttta acagaaaacg aagtaaaaag aaaaattgga
    12061 aaaaacaaaa ataatataac tgaattttat atagaaaaag gtcaaataat aactccttct
    12121 gcaaaatctt atttagctga gagaagtatt gagcttaaat atgttgataa actttcagat
    12181 gtaaacaatg aacctaaaga agttgtaaaa gaagtagtta aagagaaaaa atataaatat
    12241 actactgtat ttggcgctaa attggatgaa aaaccagaac atatgactca attaaatgga
    12301 aatcttttag tttttaaaga ccataaaaga ataatattga gaggaaaaat agattctctt
    12361 gaatcagaaa tactaaaggt acagatactt tgtcagaaac tagatatgcc aaagcttgtt
    12421 aaggaattgc aagaagtatt agactttgta agaaatataa tgagatgtga agttttagat
    12481 gaaaagctag aagactttaa acttttagga ttaactcctc aagaacttag agaacaatct
    12541 cactatccta aaaaatactt tggtatagga catgaattcc caagttatga aatgggagaa
    12601 gtagtagtag ctataaatag tataagaagt aatacacgag aggttgaact tgcagcctat
    12661 gaagcattta agggtgagta tggaaatgtt cacagagaag atttaataaa ggcacttaat
    12721 agattatcgt cagtattttg gataatgata tataaggtta gaactaataa gtataagtag
    12781 ttggaggaca taatggagaa tctattaaat gaaatagtgg aagaagtaat aactagagtt
    12841 aaaaaagaag catttataga agttgaagca tcaggaagac acgttcactt aagtagagaa
    12901 gatgtcgata aattatttgg agaaggatat actcttacaa aactaaaaga cctatctcaa
    12961 ccaggtcaat atgcatgtaa ggaaagagtt acaataactg gtcctaaagg aagtataaaa
    13021 aatgtagttg tacttggacc atgtagaaat gaaactcagg tagaaatatc tttaacagat
    13081 ggttcaactc ttggactaaa agctcctata aaacaaagtg gagacttaga aggtacttta
    13141 agtattaaaa tatcaacaca atatggtgaa gttgaacttg ataaggggtt aatggttgct
    13201 aaaagacata tacacatgac tcctaaagat gctgaaaaat tcaatgtatg tgataaagag
    13261 atagttgaag ccaaggtgat gggtcaaaga cctttaacat ttgatgatgt agtaataaga
    13321 gtgagtgaca gttttaaaac ttatatgcat atagactatg atgaagccaa tgcatgtggt
    13381 tattcaaaag gaactgttgc taaaataata aagaaagcat aaatttaacc tactatataa
    13441 attacctcta gggaggattt aatatgaatt atgataacct tgtagattta ataacggaag
    13501 aaatctataa aaaaataaat agtaatgaaa taaaaatatc taataaacct aaagctgtga
    13561 ttgtatttga acaagataac aataaattta acttattaaa agatgaattt gaaatactag
    13621 aatttgataa aagtataagg gaatgtgaaa tagtgatagt ttcaagactt tgtatgaggg
    13681 ggctatcgaa tatagcactt ggaaattcaa caagtgatga agaaagattt atactaaaaa
    13741 tgattatgaa aggcaaaaaa gtatatgtcc tagatgaagg aattgaatac aaaaaatata
    13801 aggatactgc accaaaagca ttatataaga aatttatgtc atttgaagat gaaatatgta
    13861 aatttggtgt agaaattata aaagacttaa atgttataac taaaaataag ttaaatataa
    13921 agaaagaact agaaagtact agaggttcta aaaaagaaga tacaaaagtt ttagataaag
    13981 atgttttaaa tttaaccagt gtagaaagtg taaaaataga taatgaattt agtttggatt
    14041 taaggaataa aaaacttata tcggaagctg acttaagaaa accaactata aatggagtta
    14101 aaaacatatt agttaataaa aaaagtataa taacaccatt agctgtagat tttatgagaa
    14161 tacaccatct caaactaaag aaactgtaag gaggtaattt tatggatata ggaaaagttg
    14221 taggtaatgt ttgggctaca agaaaagatg aaaaattatg tggccaaaaa cttttagttg
    14281 taaaaatttt aaaaactaaa gacacataca aggaaggctt catggttgca gccgataatg
    14341 caggagcagg aaatggagac ttagttctta tatccaaagg ttcctcagct agagagtcga
    14401 ttgaaaaatc tcatgttcca gtagatgcaa ctatagttgg agtagtcgat tcgtttgagg
    14461 tgtttgatga ataaaagtat aggagctata gagtttaaaa gcattgctaa aggtatagaa
    14521 gtatccaatg aaatgataaa gaaatcttca gttgatgtat tgtacttaaa aagtatttgc
    14581 ccaggtaaat ttctaataat agtaggtgga gaaacttctt atataaatga atgtgtagat
    14641 tatggaataa aacttggaga aggatatatt gtagataact tcgtaataaa tgcaatttca
    14701 caagaaatat tagatggatt taaaaataag tatcaaaagc tagatagtat agtctctata
    14761 ggcgttgttg aaagtagtaa agtatgcgct ggaataaaaa tgcttgataa gactttaaaa
    14821 tctggcgact tagtattagt aaaacttcaa ttatcttttg ctataggtgg caaattagta
    14881 tatgtagtag caggagattt aagtagttta gaatatgcat taaaagaaag tgaaaatgta
    14941 gtaagagaaa aagaagtaat atataagact gtaataccat cagtagacag tcaaataata
    15001 aaaagtctaa taaaataatg gggggaatta tgagtataaa cgaaattata atctatgtaa
    15061 tggtagtctt catggtactt ggagctatag ataaatgtat aggtaacaaa tttggtcttg
    15121 gagaacaatt tgaagaaggt ataatggcaa tggggtcact tgcagttgca atggttggtg
    15181 ttatatgttt agcaccagtt cttgctgatg tattaagacc aattgtagta ccagtattta
    15241 atatacttgg agctgaccca gctatgtttg ctggaagttt attagctaat gatatgggtg
    15301 gtgcgccact tgcacttgaa cttgcgaaag accctaattc tgggttattt ggtggattaa
    15361 tagtaggggc tatgatggga cctacaatag tatttataat accagttgcg cttggaataa
    15421 ttgaaaaaga agaccaaaaa ttcttagcaa caggaatact tgcaggtata ataacaatac
    15481 ctattggagc attcgttggt ggattagttg caggattcga ggtaatgatg gttcttagaa
    15541 acttagtgcc aataataata tttgcagtag ttatagcact tggattatta aaattcgaaa
    15601 atgctatgat aaaaggattc acatactttg gaaaaggtgt agtaatagtt ataactttag
    15661 gacttgctgc tgccatagtt gaagctttaa caggaatagt tgtaatacct ggaatgaaac
    15721 caatcagtga aggtatagaa atagttggag atatagcaat agttttagca ggagcattcc
    15781 cattagtgtt tgttataaca aaggtattta ataaaccatt aatggcttta ggaaaagttc
    15841 ttggaatgaa tgatgtatca gcagcaggcc ttgttgcaag tttagctaac tgtataccaa
    15901 tgtttggtat gatgaaagat atggacaata gaggaaaaat aataaatgta gcattctctg
    15961 tatcagcagc attcgtattt ggagaccact taggatttac ggcaggattt aattctaata
    16021 tgataacacc aatgatagtt gctaaattag ttggtggtat aacagcagta ttgttagcta
    16081 tggttatcgc aaataagaca ctaaggaagg agaatgtata agatggatat atcaaatata
    16141 gataaaaatt taattgaaac attagtaaga caaataatag aagaaaaaat aagtggaact
    16201 aaagacacag tagattttgt tagaaataaa gacattagtg gaataacttc tataaagtta
    16261 ccaactgtaa aagttagtga atctgataga ttagatacag ggaatccaag tgatgtagtt
    16321 tacacaaaag atttatttac attagaggaa agcccaagat taggttgtgg tatgatggaa
    16381 atgaaagaaa caacttttga ttggactcta aactatgatg aaatagatta tgtaatagat
    16441 ggaactttag atataataat agatggaaga aaggtttctg cttcttcagg tgaattaata
    16501 ttcataccaa aaggaagtaa gatacaattt tctgtacctg attatgctag atttatatat
    16561 gtaacatatc cggcagattg ggcatctcaa aattaataaa aatcaagtta agttaataat
    16621 aatcaaataa ataatttaaa atacaaactc ttaaataaac acacatattt aaaaaagcta
    16681 tgaattaaaa ttcatagctt tttttataaa gaatgttata ttttagacgt attttttcta
    16741 tatttactgg gagacatatt atatttttta ttaaataatc taataaaatg ctctacattt
    16801 ggataaccaa tactatttta ctgataatct tttccttatc actaatctta cattttaaaa
    16861 tattattttt actcgtaata ttatcataag aatttgtttc ttatttaaaa ctaattagaa
    16921 ttataaagtt tattgccatg ttattaataa aataaatcca gttaaatcga gtgctagtta
    16981 aagtaataag aaacttttac aatggtttcc tttatcgatt gaaataaaag ttaatatggt
    17041 ataatatcta aagagagaat cttatcaatt agaatatgca tagaaacaag tggcttaaaa
    17101 ggagacgtta tatggaagag atagtaaaga aggaacaagc aagtatgaat gatatttttt
    17161 tgtatcttaa agctaaatca aactcattat ataaatttgt aatattatat aatgagttta
    17221 tcaataaacc taaagattat ggaactgggg aacttataaa catggttgaa gtacatatgc
    17281 taacagcgat tgaagaaaat ccaggaataa gtccaactca gttagctaaa atatggaaca
    17341 gaactaaagg tgcaatatct caaactatat ctaaattgga gaaaaagggt tatataacta
    17401 aagaaaagaa agaaggaaat gcaaaaacta tattactata tccaaccaca tcaggagtag
    17461 agttaagtaa ggcacataaa ctatatgaca ccatagatgt tacaagaaca tttgaatact
    17521 taatcaagga gtgtacatta gaggagatag atagttttta taaagtaatt gatttgttta
    17581 ttaatttatt agaagaatct ccgcaggaaa ataatgattc tgaaaaagtg tagctaagat
    17641 tgaagttgaa taaagagaaa tagatattat agaagtagta cttaagtaca cattatataa
    17701 aaatataata ggtataggat taaaaagttt agtggtttaa agtttaacca ctaaactttt
    17761 ttaatgtcta taatctacta ttatttaatt tactaaaaat aaaaaaactc attatattaa
    17821 gatgaaaata gaaaaataac tagaaaaata ctataatata tcagcttaat acagaaaaaa
    17881 gttaaaaata ttgagaaaaa taaaaatgaa aacgttttat attgacatag agaaaaagta
    17941 gtgataccat ttaaatatag tttagtagct aaactatatt taaattataa taactatttt
    18001 agggggatgt tatgaattta aaacaattaa gtgaaaaata cgaagaatat gtaattcaaa
    18061 tgagaagaga atttcataag tatccagaat tatcttttaa agaaataaga acaacccaaa
    18121 gaataaggga agaattacaa aaaatagggg tcccttttgt atctattgaa ccaaatatcg
    18181 taatagctac tatacaaggc aaaaatgaag gtaagacaat tgcaattaga ggagatatag
    18241 atgcgcttcc aatgcaagaa gattcagatg ttgaatacaa atcagaatat gatggaataa
    18301 tgcattcttg tggacatgat gctcatggag ctatgttact aggacttgca catatgctaa
    18361 atgatataag aaaagattta aaaggtagag tattattagt ttttcaagca gctgaagaag
    18421 ttggtggcgg acacaaggag ataatacagt actttaagga aaatggagga ccagatagat
    18481 taatagctac tcatgtttgg tccgatattg aagctggtaa aatttctgtt gaaccaggac
    18541 ctcgtatggc tggtgggtct ggttggagaa tagacataac aggtagagga ggtcatggtt
    18601 ctagaccaga tcaaagcata gatcctataa agccagcttg tgatatagtt ttaaaagtat
    18661 cttctatacc agttaatcat ataagtgtac tagaacaatg tgttgtacat gcatgtgcaa
    18721 tacatggagg aactacaata ggaaatgtta ttccaaacaa tgcagaggta ataggtggat
    18781 atagatattt tactgaagaa tcaggcaaaa aagtgtttga cattataaag caaatgagta
    18841 atggtgttgg acaagcttat ggagtggatg tgaaggttac tcatactgga ggcataggac
    18901 ctgttatcaa tgatgaagaa gcagttaaga tggctaaaga aatagtttct aatatagatg
    18961 gattggagtt agattcttat gagccaatat gtgcatcaga ttgttatggt gaaatattaa
    19021 aagagtattc tggtttttat tgttatctag gggtaggtaa caaagaaaaa ggaataatat
    19081 atgcacaaca ccatcctaag tacaatatag atgaagatac attaaagtta ggttgcgaat
    19141 ttatggctaa atatgcagtt gaatttttaa ataaaaataa taactaaaat aaatcgaatt
    19201 ttataaatgg gggggatgaa aatgcaaggt gcattaaagg aaaataagaa aaaaagtggt
    19261 ggaggatttt taggatggat tgaaagagtt gggaataaaa tgccacatcc tctagcatta
    19321 tttacatata ttacattgat agtagtagca ttatctgcaa tagcaagctt tttaggtttt
    19381 tcagcaacaa gtcctgctac aggaaagtta ataaaagtta tgaacttaat aagtgcaaaa
    19441 ggtttagtac tatttatgca agagtttgtt aagaacttcc aaaacttccc tgtgcttggg
    19501 gtagttttag ttatggcagt tgccacagga gtttgtgaaa agacaggatt tttttcaact
    19561 gccataaaaa tgagtttatc aaaagttaag gggaatgcgg ttgtatttat aatagctttt
    19621 ataggagtat ttgctaatca agctggtgat gctgcttttg tattagtacc aactcttgca
    19681 ggagcaatat tttatggatt aaatagaaat ccattagctg gtatattctg tggatttgca
    19741 tcagttggtg gtggatttgc aacagctgta attcctgggg gatgggacgt tacgctaacg
    19801 ccaataactg tatcagctgc tcaaactatt caacctgcat ttgatatgac attattagct
    19861 agttattatg cactatttgt atctgcgttt atagtagcta cagtatcaac aatagtaacg
    19921 gttaaattta tagaacctaa attaggtaag tatactggta aaccagaagg tatagatgat
    19981 aatcaaggtt ttgaggtcac aaaagaacaa gcaaaagcag caaagttagc aggttttaca
    20041 gttttagctt atgtagcgtt attaattgct ttgtgtatac cagctaatag ttttttaaga
    20101 agtccagaag gttctttaat atttggagca ccacttatgt cttgcttaca gttttttata
    20161 gtaatactat tcttcttgcc agggattgtg tatggaatga gggttggtaa aatcaagact
    20221 gtaaaagact taaatgatat attatgtcaa tcaatggcag caatggcacc gtttatcgta
    20281 cttgcaatag ttataggtca attcttaacg ctattctcag tgtctaattt agcacaggta
    20341 ctagctataa aaggtggagc attactcaac tcattgccac taccaccaca agttataata
    20401 ctgctattct tagtactagt agcgtttata aatatatttg ttataagcgg aagtacaaaa
    20461 tggatgatat ttggaccagt atttgttccg atgttaatgc aattaaacat acatccagca
    20521 tttactcagt ttgtatatag attaggagat tctgtaacta atcatttatc accattaaat
    20581 gcattcttta taatactttt agcaacagtc caaaaatacg ataaaaatgc aggtatggga
    20641 actatattct cagctatgat accttacact ataggttact ttgtagtact tgcagtttta
    20701 gtagtagttt ggatgacaat aggtatacca gtaggaatag gtggacaagt ttggttataa
    20761 aacatattta ataattttgg gaggaaagaa aatggaaaca actgaaataa gatacaattt
    20821 tgatgaagta atagatagaa gtggaacaaa ctgtcttaaa tatgatctta agagtcaatt
    20881 tatgccagaa tctccagaag atgcactacc actatgggta gcagacatgg attttgcatg
    20941 tgctcctgag ataattaatg caatgcatga aagaatagac agaaaaatat ttggatatag
    21001 ctctcaacaa actgatgatt attatgaagc tgtatgtgga tggtttaaga gaagatttga
    21061 ttggaaaata gaaaaagaga atatattctt tagcccaggg gttgtgcctg cgttaggatt
    21121 tttcatacaa atattaacac aaaagggtga tggtatcata atagaaaagc ctgtatacta
    21181 tccttttgaa gataaaataa ctaataatga aagaaaagta gttaatagtc ctttaaagtt
    21241 tgaagatggt cattattcta tggattatga tggatttgaa gaattagcta aagatgaaaa
    21301 taataaggtg tttatattat gtagtcctca taatccagtt ggtagagtat ggaaaaaaga
    21361 agaacttaga aaaatagtag acatatgtaa gaaatatgat ttgtggataa tatctgatga
    21421 aatacattgt gatattattc gtaaaagtga aaagcatata ccattagaag tagcatgtcc
    21481 tgattataaa gatagaataa taacttgtac agctccaagt aagagtttta acttagctgg
    21541 tatgcaatta tcaaatatag taatcaataa taaggaatta caagaaaaat ggatgcttga
    21601 aacagattct aaattagcta tattcctacc taatccattc gcaatagttg ctactaaagt
    21661 tgcttataat gattgtgaag attggatgaa tcaagtaaat gagtatatag atggtaatat
    21721 taaatatgta gaagattttg ttaatgagta tatgcctaaa gtaaagataa taaaatctca
    21781 aggaacttac ttaatttggt tagactttag agggtatgga ttagatgcaa aacagcttga
    21841 atatgtaatg cttaacaagg cgaaggtatt gctagatgaa ggatacatat tcggagaaga
    21901 aggagcagga tttgaaagaa taaatgttgc ttcgcctaga acaattatag aagagtgtat
    21961 gaatagaata aaatgtgcat ttgaaggatt ataatagtaa tatctaaata atatatttgt
    22021 gtattttaaa aaaagactat gttaaaaaaa tatttcaaag catagtcttt taaagtttta
    22081 tgatgagttt aaaaaatttc aaaacgttac tattattgat aatcttcact tatatgttta
    22141 tattagaaat aattccacaa tatgctcatt ttctaaagtt gcacttttat acaccttgct
    22201 tcaccaatat catgtgtaac caaaatagta gacttattct cattcttaat aatactataa
    22261 acatcatcac aaactaataa tctagtctga taatcaagag ctgaaaaagg ttcatctaaa
    22321 agtagaatat ctggatttac agaaagtgtt ctaataagcg caactctttg tctcatacct
    22381 ccagataatt cttttggata catacttcta aaatcccata atccataagt ctttaaaagg
    22441 ctttcaactc tatctaaaga ttttttatct tttttgcctt gaatttccaa tccaatagtt
    22501 atattatcca ttatatttct ccattcaagc aaattatctt tttgaaacat atatccaata
    22561 tttccagtaa tctttatatc accactagtc ggttttaaaa gattagtaag aatatttaat
    22621 attgtagatt taccacttcc agaaggacca agcaaagtta atatttctcc ttctttcaag
    22681 ttaaaattga catcctttaa tacttcaagc tcgccttcct tagtgtagaa gttcatattt
    22741 atatttttaa cttctactat gttgtcattc attatgccac cccctataac ttttagctcg
    22801 tatatattat gttattaaca atatgaccta tttgtaccat ttttcataaa tataatataa
    22861 atattttgtt tatttacgta ttttcttagt aacttgtacg tataaattat aataaataaa
    22921 ttataaggta ggggataaat ttgagtaaca taataacgaa tttaatagag tcagtattta
    22981 ctaatccaat aactatattg tttttacttg caataactgt gtattctgtt tttaaaataa
    23041 tagaaaataa cagagtatat tcatttgtaa gttcaaaatt agacgaaatc aatagagatt
    23101 ataagacaag tgagttttat aaaaaggtaa gggatgacta ctatacatat agtaaagaaa
    23161 atccttatgc agacgtaaat ataacttcat ttatagagga agttgtatct gatttaaagc
    23221 ataataattt gccattgtta gaaaaaatta gaagtataaa aaattcatct tcaataagta
    23281 tattacttgg ggttttaggt acgtttgttg gtctttctac aatgctttta tgtgttgata
    23341 ctaaagacat tataaattca cttccttcaa ctattagtag tatgcaaact gcatttacaa
    23401 ctagtatatt tggagttgta ttttctctaa ttataggtta ctttactaag ataaaagatt
    23461 gtgaacatgt tttgatacaa ataatgttaa aatctgaaaa tttactaaca tctgaaataa
    23521 ctcattttaa gtcagaaaga atggatttaa aagtagaaga ggtaaaaaat acaataaaac
    23581 aaataagtaa gtctatagaa gctattgaaa aatttgataa aatatctaaa gatttaaatg
    23641 actttaatga tgaatttatc agtggtatag aagctttaaa aagcctttta gaaggttctc
    23701 aaagttctat taagacattt gaccagagcg taagaaaatt agataaacag tttaatattt
    23761 taaatataaa atttgtaaag ctttttgata aatatgataa tcaagataat ataaataaag
    23821 agattttatt tgatataaaa gaatcttcaa aaaatatata caatgccact gaaagccaat
    23881 tcaaaattag agattatata aaaaatataa atgcaggatt tgctctatat gaaagaagtg
    23941 ctcaagattt attaactaaa ttaatgaccc atgaaaataa aatctctcaa aatcaaaaaa
    24001 tccttttaga tgaaaagttt accttagatg actcaataaa aaatctctca agtattattg
    24061 aaaacttttc taatgaccta caagttaaac tggatatgat gtttgaaaat tctttagata
    24121 ttcaagataa gttagatgtt atgtttaata actcttttat gaatgatgaa gttcctcttg
    24181 atagtgagga attatttaag gatgatgtta ataatgtatt taatcctttt agtgaagaaa
    24241 tttatgagat agaggataaa gaaataaagg tcataggtga ggatgagtta aatgaataat
    24301 aaatatagaa gaactgttaa cagagaattt gaaaagagta gcttttggcc aacatttaca
    24361 gatttattat ctactgtatt gatggttgtg attcttatct tatttagctc agaaagtatt
    24421 tcaggttctg ttgaacaaga tttagctaag aatgtcaatg cttctgttga agaaactttt
    24481 aaaaaaagca aaatacctgt gaaggtagat aaatctaatg ggcaagtaac ttttggtgaa
    24541 aaaaccatgt ttgatgtaga tagtgatgtt ttaaaaccag aggctaaaga aatgctaaaa
    24601 atgtttgttc caaagtacat agagactatt tataaagatt atggtgacta tatatccaaa
    24661 atagttatag aaggtcatac agatgatgtg ggaagttata tatacaactt agatttatcg
    24721 caaagaagag catatagcgt tgctaaattt atagtgggag atgaaatagg tgactataaa
    24781 tacaaagata aagtgactaa gcatattatt gcaataggaa ggtcaaaagc tgaactcatc
    24841 aaaaatggtg acaatagcgt gaatagagat gcatctagaa gagtagaact aaaatacgaa
    24901 ataaatatta atcaaaataa ataattaaat ttttataaaa aaaaagaagg cagttggtgt
    24961 gagctttaag ccttctttaa ggaaaaagtt tatttatttt tgttgatact taacatatta
    25021 tagtttttag aagcagtttg caatatatta aacgtaaatg gtcgtttaaa atgcataact
    25081 ggatactact gatgtaaaat atttcatttt atataatata aaaattatac agtgtcaatt
    25141 ttctatatta ttgtactata atctattatt taaaatatta tgtaaaaaaa tatcataaaa
    25201 acgcatatca tgttataatt actagagtat tatgaatatg gaggcgtagt ttgtgataga
    25261 aataagtata aaaaataaaa aaaattctaa aattaaaact ttgatacttt tattgatagt
    25321 atctatatcg atttatttaa ctggttgtac tacatcagat aatggaaata atcaatcaaa
    25381 taataatgat tctttgtcta gtagtatatc aagtgataag aatgtaaaaa ttcattttat
    25441 tgacactgga aatagtgatg caatattgat tcaagatggt aaaactttta cattgataga
    25501 tgggggagat aatgatgatg agggattaat ggtagattac ctaaacaatc aaggtgtaaa
    25561 agatattaaa tatcttatag ctacccattc tcatgcagac catttaggtg gacttgattc
    25621 tgttgtcaaa aactttaata tagaaaatgt ttttgtatcg aatggttcag cagaaacaaa
    25681 gagttatagg gactttataa atgctttagc aaataagggt ttatccccaa gtgtaccgct
    25741 tgaaaataat aagttctatt tagaagattc ttattttgaa gttctaaata caaatggggg
    25801 agatactact aatgaacaat ctctagtcct tgtatatact aatgggaatg ataaagtttt
    25861 atttacaggt gatgctgaag aaggtaccga aaaagaaata ctacctaagc ttgagaaagt
    25921 agacctttta aaagtagctc atcatggttc tcgtagtagt tcatctcaag agtttttaga
    25981 taaagtaaat cctgaatatg cggttctttt agtaggcaag ggtaattcat atggtcatcc
    26041 acatcaggag acaatgaata aattgaaaaa aatgggagta aaagtacata gaagtgatga
    26101 gtgttcagat ataatatttg aatcaacagg tgatggagta tttacaagtt gtaaagatgg
    26161 aagttataat aaaggtgtta gagaagatgg aaactctgga aaatcaaaca gtaatacaag
    26221 taaaaatgat tcttttaaaa ataaacaaaa tacattcaat tcagaagaaa tagtgtattt
    26281 tactccaaaa ggtaagagtt atcactctat aaaaaattgt agtgggcttt caagaagtaa
    26341 aaaaatatta tcaggcacaa ttgaagaaag taagaaaaat gacccatgtg atatatgtta
    26401 tggaaaataa caaaaaaaca accaatttat ctataaagct tctgaacttt aaatcattta
    26461 aaaaataaaa taaatgcata aaaaaaataa gctataatca atagataata catttcatct
    26521 atacgaatag cttatttttt taagtatttt ttaatagcta tttaataact ctaaatacac
    26581 cagttacaat acctacaatt tcaacttctg agtcattgag cataattggt tccataaaat
    26641 cattttctgg ctgaagtctt attaaatttc cttctttaaa aaacctctta actgtagcag
    26701 attcaccatt tataagtgca actactatct gagaatttaa ggccgtattt tttttgtcta
    26761 ctacaacgta atcttcatct aaaattcctg cattaatcat actttcgcct tttactctta
    26821 atacaaaatt gttactgccc tttactaaac tggcagggaa tggaatgtat tcctctatgt
    26881 tttgttgagc taaaatgggt tctccagctg ttatttgacc tactaaagga agctctatta
    26941 tttcttgatt agctccagaa acatcattta ctttactacg ttctaaaact tcaatagctc
    27001 ttggtttagt agggtccttt cttatgtatc ccagtttttc aagtttattt agatgagagt
    27061 gcacagttga agtagatctt aaccctacag cagtacatat ttctcttaca gcaggtggat
    27121 aaccttttaa tgtgatttga gactttataa attccaatat caagacttgc ttttcagtta
    27181 gatctaaata cataacatca ctcatttcca aacatatttt gtttttattt ccatacactt
    27241 tatattttta attataacat aatccattta tatctgcaaa catttgttcc tgatttttaa
    27301 tcgtcctcaa tccattcaaa attcttgact atatcacgtt ttacattttt cttatgagct
    27361 gcataaagct gagtagttgt gacattatca tggtcaagta agttttgaac atcatatata
    27421 gaaaatcctg tttggattag agaagttgca gccgttgctc ttaatttatg gggactatat
    27481 ccattgtctc tagtagtatc aagtggaata gatgtatatt tttttaccag tgttcttata
    27541 gcctttgctg taattctctt attttgaaga gacaaaaata aagcatcttt ttcttcatca
    27601 tttaatagtt catctcttgt tctttcgttt tgcaaataat cttttattac atgttcacaa
    27661 gtatgattta taggcattaa aacttctttc ccacgttttc tatatatttt aaattcacct
    27721 cttgaaaaat taaaagagga gatattgagt tctctaagct catttaatct aagcccgtat
    27781 gtaacaaata atgctagtat agctttgtca cgtaatttag tttttctcca atataccttt
    27841 tctttctctg taagcccttc acctgtttct actgattcaa gcatcttagc aacttcatct
    27901 atctcaaggc gcttaatagc atctggctga ggtttaggaa gttttattgg attgaatcca
    27961 tcagttatat tattatctat ctgagagttt ctgtataaga atttaaatag agtagagatg
    28021 gatgattttt ttcttgctaa cgctctattg ttattttcaa aaatcaatgt gtttttttct
    28081 gtatttttgt aatatctact gcaataatca cctaagaata aatttatatc tcttgatttt
    28141 attgtattaa aatcatcttc tgtaatatct tttacacaat ctgcatttgt taattctttg
    28201 gtttcaatca aatatgaaca aaaaaaatta atatcttcta aataagcaag tcgtgtagaa
    28261 actgcaacag aaccttttaa atatataaaa taatccttca taaatacagg aagtttattt
    28321 tccaaaagta gacatttttc tatgagttca ttttctttga taacaatatt atcaggttta
    28381 tctgatttat tatgtacttt tataattttc ttatcattat tcaaaattaa acctcctaaa
    28441 actaaaatat aaatctttta tattattttt tgatatttat accaagtgag tataaatatt
    28501 taatttaatc attttttaca tttttaagag attagcagtg ctttagatga aattatcact
    28561 aactattccc taaatccaca tttaaagact agttgaattt aagtactctt tttttatttc
    28621 tcaatatatt tatagaaata ttttaaaata tattgagaaa ttttttatgc tctactaaaa
    28681 tcttttttat ataatatttt tatatatttc taacgaaacc aaatattagt aaaatatgta
    28741 tttatttaac taaagttttt aaatgggctg actaagctta aaatatagga tattatgaga
    28801 gttactattg aaaaaataaa ccctattgcc ataaagaaac ttataaatat gttatactta
    28861 actattaaac tggatatatt caagttatca aatccaaata agtatgtaat tgaacttatt
    28921 acaaaaataa ctataactat aaagctcaga ccatttaagc tattttttaa atcagcacta
    28981 ctaagtgaca tatgtgtaga tatagaatac attaaaaaca taaatataaa atacttcaat
    29041 gaatatacac taggtgttaa caacattatt cttataaaat tggaaatatc acctaaaata
    29101 atgtgtataa aaccatttat atttaaatta ttaatcatac ttacatataa ctttaaatct
    29161 atattttgag tcactttcat ataactatct ggcagtaata tcttaaaaat taaaataatc
    29221 attattgttc cacctataat aggtgcaatt cctataaaga aatttcctat actttgataa
    29281 agattatctt ttttatagct atgacttaca tatcctagta taccatcatt tttactttct
    29341 attggtctga aaaacttaac tgaatttatt ttatgcatga aaatcaagca cattatcaaa
    29401 tgactcaatt catgaacaaa cgttcctata gctccagtta caataattcc agtttttcca
    29461 aaagatgaaa agataaattt attatttaga ttttctgtaa tgctaaaaat caatccaaat
    29521 acaataaata ttcctatgaa acaaaaaata tttattacac ttatttttaa aatttcgatt
    29581 atataaatca taagttaagt cctcatgtaa aagtatttaa ctactctact tcatatccat
    29641 tacagcttaa aattttacaa gcatcttcta tatctttttc ttttactaag atatagtctg
    29701 tatcatacgt agaaattgca aatatgctta ttttgttgtc tgctaacaaa ctacttaact
    29761 tagataaaat tcctacaagt gaaaagtcta aaggacctag tatcttaagt attctccact
    29821 ccttttctga cttaacatca gaaggaatat tattatttaa acatacaaca cttaattcat
    29881 catcagttct agttatagag taaaacttct ctgtatctat ccatgttggt attttactgt
    29941 cattatttaa tctacatact gcatactctt ctaccaataa ttttaatttc ataatgtata
    30001 tttcctccaa tcttatttgt ttacttaaga ttatacatta aaagttacat aaaataaaac
    30061 atataaagta aaaaaggttc actatagtgt cctatttcta tagccaacct ttaaaactta
    30121 attaaattct aataaagaga tatattatct aggttcaatt ataagtttaa tggctgtttt
    30181 cttatcacct tcgatttcta tatcagtaaa tgcgggaatg caaactaaat ccattccact
    30241 aggtgcaaca aaacctcttg ctattgcaat tgattttatc gcttgattta gagctccagc
    30301 tcctatagct tgtatttctg caacgcctcg ttctcttagt actccagcca aggctccagc
    30361 tacagaatta ggattagatt ttgatgaaac ttttagtacc tccatttcaa catctccttg
    30421 ttaatttgtt aaagtatcca ttacctaaag tactaaatca taattgccat ttactaatta
    30481 caatcatcat ttaaagtata cttttataaa aatatacttc tataatcgtt ttaagaaaac
    30541 ctttattttt tttaaccatt ttattatgaa aaaactacaa atttctttat attctataag
    30601 cattttccaa tatttctaaa ttttttatat ctttgagcta taatttcctc tttatccata
    30661 tctttgtaat gatttaatct ttctaaaata taagctttta tttcttttga aacagcttct
    30721 acatcttttt gtgctcctcc aagaggttct tttataatct tatctattat ttttaaatca
    30781 tataaatcat tacttgtaag cttcataaca tcacaagctt ctttagcttt agatgaatct
    30841 ttaaatagaa tactagaaag cccttctgga gaaatcacag aataaactga atgttctagc
    30901 atacaaacat catttcctac tccgatacct aaagctcctc cactaccacc ttcaccaatt
    30961 acaaaagtga ttactggaac agaaagttta ctcatttcaa gtaagtttct tgcaatagct
    31021 tctccttgtc ctctttcttc tgcttctaaa ccacaaaaag ctcctgatgt atcaataaac
    31081 gttattatag gtctattaaa tttttcagct tgtttcataa gtcttaatgc ttttctgtat
    31141 ccttctggat gaggcattcc aaaatttctt tttatatttt cttttgtatc tcttcctttt
    31201 tggtgtccaa ctatagtaac attaaacttt cctatctgac caatccctcc aaccatagaa
    31261 gggtcatctt taaaaagcct atctccatga agttctaaaa aattagaaca aatatgttct
    31321 atgtattcca atgtcgtagg tctctttata tctctactta gtgttacctt ttcatatgct
    31381 gatagatgtg aaaaagcatc ttccttaagc ttttccaatt tctcattaag tgaatttatt
    31441 ttatcactta aatctatatt atttgcctta gaaatagtta ttaattgttt aatatcattt
    31501 tctaagactt ttattttttc actattattt tcaatcaaaa cagttcctcc tttatgccct
    31561 atttcttgtg catagcaaga atttgatata ataattcctt catttttctt ctatctacta
    31621 tcatgtcaat aaaacctttt tcaagtaaaa actcagaagt ttgaaatcct tcaggaagtt
    31681 tttgatttat tgtttgctct attactcttg gacctgcaaa acctatcaac gtatttggct
    31741 cagaaattat aatatcacca agcatagcaa aactagcagt tactcctcca gtagtagggt
    31801 cagttagaac tgatatatat ggcaatgatt tttcttcaag cttagaaagt gcttgagaag
    31861 tctttgccat ctgcataaga gataccatac cttcttgcat tctagctcct cctgaagcag
    31921 aacaaataat tataggtaaa ttattttcag cagcatactc tattgaataa gttatttttt
    31981 ccccaacaat agaacccata cttcccatca taaaatcagg attcattaca caaataactg
    32041 ttttaattcc atttattctt ccataacctg tgacaacagc ttctgactct tttgtttttt
    32101 ccttataaga gtctactttt tctttgtatt taggaaaatt tagtggattt tgaaattcta
    32161 catcagaatt aaactccata aaagttcctt tatcaatcaa taaatttatt ctttttcttg
    32221 ctcttaagcc ataatgtctt gaacattttg gacatacaaa tgaattttct tctatgtcct
    32281 ttctaaatac atgactatca caacctttgc aataggtcca aaatttatca tctacactat
    32341 tctttttaaa gttgtcatct aaagaaatcg taacatactt agattctttt gatgataaaa
    32401 atttttttat cataatctat ttactcctta taatgtagca aattctttct tcataaagac
    32461 atcttctata aatttggtat gatgagtatc ttctctaaac tcctcacttg ttacaagtgc
    32521 cttttgaaat tctacatttg tattaatacc ttctacaata atttcgtcta gagctctata
    32581 tattctactt atacattctt ctctagtttc agcccaacat attaacttac ctatcataga
    32641 atcatataaa ggtggtatag tatatcctgc atatacaaaa gtatcaaatc taacaccaaa
    32701 acctccaggt aaatttaatg attctatctt acctggtgat ggagcaaaat tctttgatga
    32761 atcttctgca tttattctac attcaatagc atgacctcta aaagtaatat cttcttgttt
    32821 aaaaggaatt ttatccccat tagctataga tatttgaagt tttataaggt caagatttgt
    32881 taccatctct gtaactggat gttcaacttg tattcttgta ttcatctcta taaaataaaa
    32941 gtttttatct ttgtcaacta taaactcaat agtacctgca ccgatataac ctactccttt
    33001 tactatatct actgcaattt tatgaagttt ttgtctttct tcatctgaaa ggtatggact
    33061 taaagattct tctattactt tttgatttcg cctttgcata gaacaatctc tatcacctaa
    33121 atgtattgca tttgaatgtt tatctccaaa tacttgtact tctatatgtc taggattttc
    33181 tataaacttt tccatatata aatcactatt agaaaatgct gctagagctt ctgaacttgc
    33241 catatcaaaa tttgaaaaaa gttcttcttc ttttctaacg attctcatac cttttccacc
    33301 gcctccactt gaggctttta tcataacagg atatccaatt tctcttgcaa cttctaaagc
    33361 ttcttcctta gttttcaaaa cagattctga accaggaaca acagggatat tcaattccat
    33421 catagtttct cttgctctag atttatctcc catcaaaatc atttgttctc ttgttggacc
    33481 tataaaaata atattattat cttcacattg ctttgcaaat tcaggattct ctgataagaa
    33541 tccaaagcct ggatgaatag catcacactt taatttaaga gcaagataga ttatattttc
    33601 tatattatta taactcttac ttatattatt tggtcctata caaatagatt catcagctat
    33661 atatctatga aaacaatctt tatctatttc agaatagata gcaacagttt ttatacctag
    33721 ttctttacat gttctgatga ttctaactgc tatatcgcct ctatttgcaa ctagtatttt
    33781 ttttatcaac ttaaatacct cctactacaa tggttttatc ttaaataaag gttgattata
    33841 ttctactagt tcttcattac taactaatac ttcaattatt tcacctttaa tttcgcttgt
    33901 tatctcattc ataagcttca ttgcttctaa tatacataaa gtgtcgcctt cttcaactac
    33961 atcgccaact ttaacaaagc tatctgcatc tggacttgga gaatcataga atgttcccat
    34021 caaaggagct actactactg atagattctc attagatgtg acaggtgtat cgtttacaac
    34081 tggcttttct aaagaaagat ccactactga ttcttgttgt acacttaaaa caggtgaact
    34141 tgtagattga gcttttttag atacttctaa tcttaaatcg ctactttcaa gttttacata
    34201 ttctaagtta gttgagtcta tggcctttaa taattcctta atttcattga tgttcatttt
    34261 gttaccccct tgtattatta cctggtgcta atattatatc acaaaaaaca aatttataaa
    34321 agaaaaagtg ctgactagag aatcaacaca tcaatatatc ttttaatttt aaattaacaa
    34381 ccagtaactt atatttgttt taattttcca ttattaatat aaattataag tataaaatga
    34441 ataaaattca atattttttg caaaatacaa gagaatacaa attaatacct cgggaatttt
    34501 taagacaaat accaatatta tgcaatttag acttcgcttt ctttcaaatt agtatcattt
    34561 tctttatttt tacataggat gtactttaat ttagctatca aaacattact atataggtta
    34621 aaagttatta gaattgcagg aattataaag taaactctag cacctataaa accaaaaact
    34681 actgtaataa gtattaatct tttatttaaa gtaaaaaaag gtatattaaa aatacttttg
    34741 tcggatgttt ctggagtata agaatatata actccaaaaa tcaaagtaag catacaaagt
    34801 ggaagtagtg tataacttga aattattccc agaaatccta tactttcaaa atatttgcta
    34861 gcactatttg tataaacaaa catgtatcca tttaaatatg cgcatataca tgatataatg
    34921 ccaaaagttc cccctaagga ttgagtgtac caattaaaaa atttagattc catatacctc
    34981 acctcctaaa actgtatata taatgatatg catttaaata gtaaaatata tctttatatg
    35041 tttctgaata aaattttgtt aatttcatta atttatgttt aagaaatttg aagtatagtg
    35101 tagtatatat taatgtagat gaagttttat agtacttatt tcatagtgct taatcaagtt
    35161 atcttattat ggattcaaaa gttgtttgta gggttttata ttttgttgag ctgaaaaatt
    35221 tattataaat gaagggatgt tttcgctttg gtagatacat tagaatttgg tcttaaaatt
    35281 ttatttttta tattatcaat aatttggatg ggtaaaataa tgattttaag aactgataag
    35341 caaatagtaa tcaatccttt gcttattggt atatctgcag tccttgtgat gttgcatacc
    35401 tcacagagta acattgaatt ttttggatta gatgtccaat atataagaat agttttgtat
    35461 ataatatatt cactgataat tttaatagga atatgggcta ctaacagaag aaatggaatt
    35521 ttttagataa tctatttcta taaaatttaa aggttgctag taacaaagca ctaatattag
    35581 cgttctgtta ctaacaacct ttattttgat tatacactta aattgaaaat agaatgattg
    35641 aataagttga catattattt tacattgaca gtgattagaa cttcttgaga tgtatttaaa
    35701 tcatttttgt cgcttagtat aacttttacc acagcttgac ctgttttatt acctttaatt
    35761 atcatatctt cacatgaaag tacactctta tcacttacag atacaataaa gtctaaatct
    35821 tttggaagat tatcagtaac ttcccagtat ttataataac tatcaatttt cttcgtaatt
    35881 atttttctca aatcaatact ttcacctttt gctatatcaa cattatgata tttagagtcc
    35941 agtttatact gattatcttt tgtatcaata aaatttaaat cgcaatttac atccacacca
    36001 ttatgagaaa gagaatttac tacatcgtca tcaatcaaat aattattggc aagatttatt
    36061 gaatccatat cttttagact taaagcttcc ttaggaacct cttttatatt attattccaa
    36121 aaaataaaat tgtctacatt ttcaagatat gatattcctt ttaaatttga aatgctctca
    36181 ccagttgcat aaaattctcc atctaaagcc tttatattgc ttatagtcat ctcatctggg
    36241 tttatatatc tttttacaac tgctcttaaa tttgcatcag gaaaaatatc tttaagttga
    36301 ttttcgctca aaaccttatc atttgaattt attggactta aagctgctgc agaaataata
    36361 aatggtatta aattagctaa attcatagta ccctcccaca taaatttttt attataataa
    36421 tttaatttct atgagagggt tctgtttcct tgttatatat acctactttc gtacacttta
    36481 ttaatataaa ttaaaataaa tttctactat taaacttata aacatattac tagttacgtt
    36541 ttttagcttc acatctaaat attcgcatac cttgtgacat aaatttgtct tcatactctg
    36601 ttgtaacatt attttcatac tcactatttc ctaaatcaag agaaatattc tttaatagcc
    36661 aattatttgc agcaatttca tttaaactaa actcgaataa tttttcatta tctgttttaa
    36721 agtgaatttc gccatcatct tccaaaaccc tattgtacat ttctaaaaat ccagaatgag
    36781 tgagtcttct tttactccat ctatttttag gccatgggtc acagaaattt atataaattc
    36841 tcgataattc tttagcttca aagtaatcca aaatattgct tacatctccc cataaaaata
    36901 gtatattatt caaattagaa gcatctgctt tttcaacagc ttttaaaaga acttcttcct
    36961 taagttccat ggcaatataa tttatgtcag ggttctgctt agctaaagtt gttataaact
    37021 taccttttcc agtcccaaat tcaacatgta ttggattatc atttctaaac tttaaattcc
    37081 acttgccttt taatttgtct atatcgtctc ttaaaacata ttttgtataa cttaaaagct
    37141 tttcatctgc accttttttt cgtcttcttc tcacaataat tctccttaca tataaaaaat
    37201 tagtaaaata catttttatt atacattatc tcaccaaaaa ataaaaagaa ctctcttaaa
    37261 atagaaattt atttctataa agagagttct aaacttacta aaaacttaat cttagtggca
    37321 ccctgaacaa ccagagcatc ctcctccacc ttgatctttc atattttcag gaataactct
    37381 aaatccaaat cctgtatctt ctattattat atctccatac tgactatatt catcagagct
    37441 cattacaaat tgtaattctc caacttcata agttaagtca tcttcttttt tctcatctaa
    37501 agctaatcca aaagaaggac ctccacaacc aacacctgca aagtatactc ttatattatt
    37561 aggtttatct tgattgtctt ttaagatact ttttaaagta tcaatagctg tttctggtaa
    37621 agtaattttc atcaaatcat tccctttcaa atattttatt aaaacaatat ctattatata
    37681 cccatacaag gtacataatc aattagtttt tgcatatttt actaaaactt ctacctaata
    37741 ttacctaagg ctttaaaata ttttatataa tattttatta ccttaaataa ataccctatc
    37801 ataaa
//
LOCUS       DAMJVX010000020        34000 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMJVX010000020 DAMJVX010000000
VERSION     DAMJVX010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34000
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.022"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(<1..528)
                     /locus_tag="PC407_000974"
     CDS             complement(<1..528)
                     /locus_tag="PC407_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496433.1"
                     /translation="MSNQIKDFDITNDMFQESIPITTKTILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLVKAANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKL"
     gene            complement(938..1114)
                     /locus_tag="PC407_000975"
     CDS             complement(938..1114)
                     /locus_tag="PC407_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496434.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1168..1264)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2060..2461
                     /locus_tag="PC407_000976"
     CDS             2060..2461
                     /locus_tag="PC407_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HDF2496435.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            2458..3525
                     /gene="rsgA"
                     /locus_tag="PC407_000977"
     CDS             2458..3525
                     /gene="rsgA"
                     /locus_tag="PC407_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2496436.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKSGCTVALLGSSGVGKSSLINC
                     LLGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKTIRR"
     gene            4061..4759
                     /locus_tag="PC407_000978"
     CDS             4061..4759
                     /locus_tag="PC407_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496437.1"
                     /translation="MKISSQYRSQYSFRHESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINEAIKNLYKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            4861..5409
                     /locus_tag="PC407_000979"
     CDS             4861..5409
                     /locus_tag="PC407_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496438.1"
                     /translation="MEGLGFYAAALSGSSYQRIGFGKLDPIEVIADGDWISYKQAQDT
                     LTVIRNFLNSFDWRNASEMERANRAAKLVTEAKYVDSKYCNIVYGNLVDKRGVCGSFA
                     SSFHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRSDGSEISAFGGALDFDYRKLK
                     DATREMTTYYDAKALSILGFNQ"
     gene            complement(5810..6766)
                     /locus_tag="PC407_000980"
     CDS             complement(5810..6766)
                     /locus_tag="PC407_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2496439.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDNNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSYGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIQFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENKSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            7469..8464
                     /locus_tag="PC407_000981"
     CDS             7469..8464
                     /locus_tag="PC407_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2496440.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKTSVIVLKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            8442..9305
                     /locus_tag="PC407_000982"
     CDS             8442..9305
                     /locus_tag="PC407_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2496441.1"
                     /translation="MSIMSNYNEASSNYSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            9302..10096
                     /locus_tag="PC407_000983"
     CDS             9302..10096
                     /locus_tag="PC407_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2496442.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISKLNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            10381..11760
                     /locus_tag="PC407_000984"
     CDS             10381..11760
                     /locus_tag="PC407_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2496443.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKELLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            12215..12928
                     /locus_tag="PC407_000985"
     CDS             12215..12928
                     /locus_tag="PC407_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2496444.1"
                     /translation="MDTYFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSAVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(13200..14738)
                     /locus_tag="PC407_000986"
     CDS             complement(13200..14738)
                     /locus_tag="PC407_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2496445.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVEEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            15621..16076
                     /locus_tag="PC407_000987"
     CDS             15621..16076
                     /locus_tag="PC407_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF2496446.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            16125..16709
                     /locus_tag="PC407_000988"
     CDS             16125..16709
                     /locus_tag="PC407_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HDF2496447.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSREQMDEVKLAMGFKL"
     gene            complement(16714..17037)
                     /locus_tag="PC407_000989"
     CDS             complement(16714..17037)
                     /locus_tag="PC407_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2496448.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLESL"
     gene            17189..17707
                     /locus_tag="PC407_000990"
     CDS             17189..17707
                     /locus_tag="PC407_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2496449.1"
                     /translation="MNFVELTKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGRQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(17883..18551)
                     /locus_tag="PC407_000991"
     CDS             complement(17883..18551)
                     /locus_tag="PC407_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496450.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            18666..20081
                     /locus_tag="PC407_000992"
     CDS             18666..20081
                     /locus_tag="PC407_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2496451.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNSKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQYVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            20304..20762
                     /locus_tag="PC407_000993"
     CDS             20304..20762
                     /locus_tag="PC407_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2496452.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            20759..21343
                     /gene="clpP"
                     /locus_tag="PC407_000994"
     CDS             20759..21343
                     /gene="clpP"
                     /locus_tag="PC407_000994"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2496453.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            21665..22741
                     /locus_tag="PC407_000995"
     CDS             21665..22741
                     /locus_tag="PC407_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2496454.1"
                     /translation="MIAISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            22808..24847
                     /gene="bclA3"
                     /locus_tag="PC407_000996"
     CDS             22808..24847
                     /gene="bclA3"
                     /locus_tag="PC407_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HDF2496455.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGGTGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGGTGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGATGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGVTGADGATGATGATG
                     ATGPTGADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLV
                     GPTGATGATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATG
                     LAGATGATGPIGATGPTGADGATGATGATGPTGADGLVGPTGATGATGATGPTGPTGA
                     SAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAF
                     SMPRDGTITSISAYFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPL
                     TGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            25016..25777
                     /locus_tag="PC407_000997"
     CDS             25016..25777
                     /locus_tag="PC407_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF2496456.1"
                     /translation="MKNKIETSMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            26086..26364
                     /locus_tag="PC407_000998"
     CDS             26086..26364
                     /locus_tag="PC407_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496457.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGEYIYIPRKSNNKKEWGSSTS
                     TREDLSIRNMQIYEDYQVGYDLKYLSEKYYLSLKSIQRIVLQEKRKDS"
     gene            26657..27232
                     /locus_tag="PC407_000999"
     CDS             26657..27232
                     /locus_tag="PC407_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HDF2496458.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPETLEQFHTNDYNLAKQYAMNTELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            27670..28380
                     /locus_tag="PC407_001000"
     CDS             27670..28380
                     /locus_tag="PC407_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2496459.1"
                     /translation="MISIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSSGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            28374..29690
                     /locus_tag="PC407_001001"
     CDS             28374..29690
                     /locus_tag="PC407_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2496460.1"
                     /translation="MLTNLSIGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIFIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMI
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYIDNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIENND
                     ICSIFANSLDNAIEACMDINNELEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            29702..29842
                     /locus_tag="PC407_001002"
     CDS             29702..29842
                     /locus_tag="PC407_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF2496461.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            29901..30482
                     /locus_tag="PC407_001003"
     CDS             29901..30482
                     /locus_tag="PC407_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF2496462.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNISVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNNDQ"
     gene            30559..32337
                     /locus_tag="PC407_001004"
     CDS             30559..32337
                     /locus_tag="PC407_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496463.1"
                     /translation="MNLLNIVESIVSCIKGLARRGINMSSIKVISDFITKIIRKNILF
                     AITVIIINYHLIQRWTHISNRGISFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTV
                     KILCRNNFDYFLGISVAVTRNNFIFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILVFE
                     WFLPLTILSIVISFISVIINNLILKITLSSVFLYLTSTKMLDRVIDIKEPFFKALFKS
                     LNIFEDQSYAYYSDFVGKVNNSAYVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLI
                     VIVVYSILFIGLNYISIDMSTYRDEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNI
                     KDRIVLLNTSNEELTNINMILDEIFDIKYIKVNSENIKFNKKKDLIEVNLKESLEPND
                     EVTLDIEYEGYINVLTSWNQNKYIATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSK
                     NNVYSNLPLLNKTSNFLKKDYIFEGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMD
                     IEEKIKFLIETRNEILGDSKASVDAKKYVDEVDNVMESIKRKKINMILVADLPSNEFL
                     YEDSKGEKISITIYHWLSGNILIIDK"
     gene            32352..33224
                     /locus_tag="PC407_001005"
     CDS             32352..33224
                     /locus_tag="PC407_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2496464.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYIPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINNKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            33225..>34000
                     /locus_tag="PC407_001006"
     CDS             33225..>34000
                     /locus_tag="PC407_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496465.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYLGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVFYIARKSTVL"
BASE COUNT        12918 a         3860 c         5476 g        11746 t
ORIGIN      
        1 taacttatat tttttaaaat cttcttcaat tttattttta ttacttttat taatgttaac
       61 agaatctctt ttaaatttat caatatattt ttctatttca ttaatattat ctttgcttac
      121 aactatacca ccttttaaat taaaaacttt tttgaaattc ttttcagaaa atccttgaat
      181 aaaataactt tgtccatctc cattaatact atcttgagta tcaaatactt caatttcttt
      241 gttaggtatt ggtatatcaa aattccaaga ttcctttagc attgaaatat tagctgcttt
      301 tactaaaaaa agattatata ggatacttcc taataacaaa acacctatta atagtagtgt
      361 gattaataca atttttctct ttttcatttt tcgttcacaa ccttttgaaa aaacatgatt
      421 taattcatca tttgaaggca tgtcttcaag aatagttttt gttgtgatag gaatgctttc
      481 ttgaaacata tcatttgtaa tatcaaaatc ttttatttga ttactcaata tccatttctt
      541 ctaatatttc ttttgatttt ttctagtaca ttgaatactc ttatatacat tctcctcttt
      601 taaaattaaa agttataaaa gaataagtta tataaaaaat tattatattt tttaatatag
      661 cttatttgca aatttataat tttgttaatc taatccttca aactaaaaaa tcatttatgt
      721 ttacagaatt tcattttaag agatgtttat tttcacatag ataagagaat ttaagttctc
      781 atatttatta tttgatacaa atttacatct ttattatatg tattttaaat agaaaataaa
      841 attatgaata ctagaaatct atcttaatta gctttctaac ataaggctat ttaaagtaga
      901 taaaactaaa aagggttctc tagaaaacca aatctattta attatactta cttactaata
      961 ccatcacttg atgcaaacaa gattctacca cattttctcc gtactctagt ataaaatcat
     1021 ctagttgtgt atctataaaa tcacaataat catatgaact accaatataa gcagtcaaca
     1081 tatgtgtaca tctatccatt ttatctctaa acatatatat tattatcccc ctaaaaaact
     1141 ttcataaaaa caaaaagaat atctcttttt ggcaactggc tgacataact catagtttat
     1201 tttataagcg ttagtcccta tagctttgcg tcactaaatt tctctagttt tgccgattta
     1261 gttttattct acaattacaa taatacaata gaattgctta ttattcaata tacttatttg
     1321 aaaattaaat aaatttacaa atatgaacgt tattttatat aaaaaaaata gactattcat
     1381 gtagaatata tggaaaaact tgattgtagg ttttgtatga aaagacagtt gttattaatt
     1441 taagtgagat aaattgagca ataggcataa aatgagaaaa caaataatag caattaattt
     1501 ttattattaa tactaggtga acattaaatt ttaaaatacc actagatttt atagaataag
     1561 aaaatgagac aaacataaat atatgaatat attaaattat acttgaacat acttatattt
     1621 tatgataaaa ttttaataat aattattagt gaaggagtgt cttgcctgat gtcagagtta
     1681 tatataatgg taaccaacaa ataaatagtt ggagacgcat atgggttctg tttaatcaga
     1741 atttttaagt gtatttttga tacatttgat tgttgtgtct tactctgcct ttatagataa
     1801 ctaacattag acaacaaata ataatattac atattacttt gttaaattgt ggtgtagtgg
     1861 gctttaatct gctacacttt tattaatttt acacagtatt attttgaata taattatgtt
     1921 gaaatagaag atactaaatt ttctagtcca caggggtttt ctgtttttgt gtaggtaaat
     1981 atgtatacca cataaaaagg cagatataaa tacctgtgga ctttttttgt agaaaataca
     2041 agatacggag gaaataaaaa tgaattataa aaatagaaaa cgagcttatt atcaggaaaa
     2101 ttcctgtatg gataacttta tttgcaaaaa ttgtggttgc cttgtagttc ctgaaggagc
     2161 tggtacacaa catagaaatc attgtccaca ttgtttacat agtcttcatg tagatatcat
     2221 tcctggtgac cgtgctgcag attgtgatgc taatatggaa cctattggag tgtgggtgag
     2281 aaaaaatggt gagtgggcaa ttattcatcg ttgtacacgc tgtggacatc ttagttctaa
     2341 tcgtattgca gctgatgata attcagtgaa attaatgtca attgcattaa aaccactggc
     2401 acagccccct ttcccactag aaagaatcat tgaaaagttg gaaaaggagg aaacagaatg
     2461 aatggtatga aacaaatcaa tttacaagac tatggtctaa ataggaattt tattactcaa
     2521 agtatggaat atcacgattt ataccttgct cgaattatct cgcaggaaaa gaacttttat
     2581 caagctgtat gtgtaaatgg tgtactgttt gcagaggttt caggaaaact gcgttttgag
     2641 gctactatac aatctgattt tcccacagtt ggtgatttcg ttatgttgga tcgttgcaat
     2701 aataaaaatg gtaatgctat tattcatcat gtgcttcata gaaaaagctt gtttgtgcgt
     2761 aaggctgctg gaaaggtaaa tgaggaacag gtggttgcag ctaatattga cattgttttt
     2821 ttgtgcatgt cactgaataa ggattttaac attcatagat tggagaggta tctgtcttta
     2881 acatgggaaa gtggagctat cccagtagta gtacttacta aatcagactt atgtgacaat
     2941 gtgcatcgta aattacaagt agttaattct atagccttgg gtgtagatgt agtagttacc
     3001 tcatctatag agccagatgg ttataaacag atttctaagt atatcaaatc aggctgtact
     3061 gttgcattac ttggctcttc aggagttgga aaatcttcat taattaattg tctgctagga
     3121 ggagaatttt taaatacaaa ggaaattcgc atagatgatg ataaaggaag acatgccacg
     3181 actagacgag agctattttt aatttcaggt ggtggtattg ttattgacac accaggtatg
     3241 agagagttgg gaatgtggga tgtttcagaa ggtttagata aaagttttgc agatatagaa
     3301 gcttatttcg gaaaatgtca ttttcgtaat tgtactcaca cctcagaacc aggctgtgct
     3361 atctgggaag ctattgctca tcaagaactt tcgcctaaaa gatgggaatc ctatcgtagg
     3421 cttaaaactg aagcagtatt tgcagacaat aaaagcgaat acttagcaaa aaaagaaaag
     3481 aaatttaaaa atatctcaaa aataaataag acgattagaa gataagtgta gtttttttgt
     3541 tcattctatg agtctatata ctattaatgt attaaatttg ccattaattt gttactttaa
     3601 tgtgattttg atggcaaatt tggtttgtgg tattatttaa agcaatttat taattttttt
     3661 aataaagttt gattttttat atgatgatat atatatgtga taatgtatac atatttgtta
     3721 cgatagtctc ctttataaag aggtcttatg taagataaga aaaggcatat ttatactaat
     3781 acataataga gtatatgctt atgtatgata gagatgtttc ttaagagcaa aactgcaata
     3841 ttgctaattt ataaagtaaa aatatatgaa ttataaaata caaataagat atttataaaa
     3901 attattgttt taatttataa taaaaatttt ttgttgaaaa caatgctcga acctataaaa
     3961 ctattaacta atatttgtaa atgtatactt ttgatgataa aatataatta agttaagttg
     4021 gtttttataa acataaattt attttagaga ggtgaaataa atgaaaatat ctagtcaata
     4081 tagaagtcaa tattcattta gacatgaaag taatataaat aatacaagaa taaatgaaag
     4141 tatggttaag aaaaatgaaa ctgtaggaaa agacacttat ttatctaata ttatgaaaca
     4201 aaagcaagaa cttaatgata gaattagaga tttaaaatat agacaagagg tttatactaa
     4261 aaaaataaat gaagcaatta agaacttata taaatcagaa ataagagaaa caactaataa
     4321 cttttctaat atagaaatag gtattaaaaa tagcattata gaagataaaa ataaaagtac
     4381 aatgttagat gaaaattcaa cttatctaaa tacaaatgat gaaaaagaat ctttaatcac
     4441 taaagagtct aatgaaaaaa ttgaagaaga aatattaaat gatgaaaaat tagaagagtt
     4501 agaacaaaaa aaggattata aagaggattc taataaaaaa gagaaagtat ctgaggactt
     4561 atctttagta ggtaaaactc gtgaagagct tgaaaatatg cttaaaaatt ttataaattt
     4621 aacacaagaa gaaataatga aacttgagtc gagaatagaa aagttagata aagatgctga
     4681 agaatacaaa caaaattcaa agactaatat atttgataaa acagatgaac aaaaaaaaca
     4741 tataaatgta ctgatttaaa caatatgttt taaaaaaata agtttaattt actctatgga
     4801 ggaaatccaa atggagttga gcttcgagca gatggtaaat attattatat aaataagggt
     4861 atggaagggc taggatttta tgcagcagca ttgtcaggta gctcatatca gagaataggc
     4921 tttggaaaac ttgaccctat agaagtaata gccgatggag attggatttc ttacaaacaa
     4981 gctcaagata cacttacagt aatcaggaat tttttaaata gttttgactg gagaaatgca
     5041 tctgagatgg aaagagctaa tagagctgca aaattggtaa ccgaagcaaa atatgtagac
     5101 tctaaatatt gtaacatagt ctatggtaat ctggttgata aacgtggtgt atgtggaagt
     5161 tttgcaagta gctttcattt attaacaagg ttaatgggaa tggattcact atctatatta
     5221 aatccatcat taaatcatgc ttggaactat atacaaattg atgggaaatg gtataggtct
     5281 gatgggtctg aaatatcagc ttttggtgga gcattagact ttgattaccg taaattaaag
     5341 gatgcaacaa gagagatgac tacttactat gatgctaagg cattgtctat attaggattt
     5401 aatcaataaa aataggggag gctttataaa gagccttttt ttattgattt gaaatttaat
     5461 aaggaatttt atatgaaaat ttaatcaata aaagcaaaaa aggctcttta taaagccttt
     5521 ctaagtgttt ttttaagtaa aattttttat atcaacatca gttttaatca aaaaatataa
     5581 cattttggac ttacttttaa aaataaaatt attaaaatat aatattaatc atgttaataa
     5641 tgtttttagt ttattatatt ctataatagt ggtcatgttg actattgtag ttttattaag
     5701 aatagttggt ttatataaat atatgcaaag atagcttgtt gtttacttta cttattagta
     5761 tataggacaa gatactctaa taaatttgcc tattttaata tcttttgtac taaaaccaac
     5821 taaagtttcc tttattattt cgttttgaat aatacaaagc ataatccgat tgtttgtata
     5881 aagactggaa agtatcagat ttattttcta atttagatat acctatagaa caagaattac
     5941 tctctagaca taattctaaa actttctttg ctttattttc aacattctct tttgattgat
     6001 tactatattc aaaaacagca aattcatctc caccaagtct tccaataata gcatcattta
     6061 cgaatacttt agtcaaaata tttgaaaact gtattagtat ttcatctcct cgaacatgac
     6121 caaagatatc atttacctgt ttaaaattat ctaaatcaaa gatgtaaaag tatccatatg
     6181 aaacagtttt aagcatttct tttatgtgta cttcaaaaaa atgtctattg tatagtccag
     6241 tcataaggtc tttttctgat tttagtttaa attcatttgc cattgctagt gcttcttgtg
     6301 ctttttcagt tattgtttta gggtaatact catctttcat ttggtcaaag tttggaatag
     6361 aatggtgaac atggataagt ttaatcatat catctgtaac tctgtatacc atagtaaatc
     6421 gagtatccat ttctacatag atatcttttt ctaggtaatc tttttctatt acccaaagag
     6481 taccatatac tataaaagct tcgtctgtaa ctttggtaca tgaataccac tcatctataa
     6541 tctcaaagtc tatttgctct gattctgttt ggtctttttc aagacctata ataaattctt
     6601 ctaatgtttc gtaaaattca tgttttcctg ttccaattaa tgcaaggttg ttatctaata
     6661 aggaaataat aaatttaagg ttttcttctt ctggatttaa aatataaata tgccaaattt
     6721 tttttgataa atctcttaat tgtttttgta ttgtattatt ttccatagat taccccctta
     6781 agatttacag tttatatttt actgtattat aagttgaact cttattacaa tattattata
     6841 atatagaact aaagaaatta catcctgtat aggtaaaagt atatctttta caataaaaat
     6901 attacagtaa aataaccagt tttaatatac gtattatatt tttaatttat aaacgaatat
     6961 tataaatgaa atttaaattc aaccctgaag aaataagcta aatgtattag tatataaatc
     7021 catacttgaa cataaataac ctttatgata tgatatcaat aataatatta tgagaggagt
     7081 gccttgatgt cggagttata taatggtaac caacaattaa atagttggag acacatatat
     7141 tctgtataat cagaaatttt aagtgtattt ttaatacatt taatttttgt gtcttattct
     7201 gcctttatag ataactgaca tatgacaaca aatagtattt aaaatatata ttactttgtt
     7261 aaattatggt gtagtaggct ttaatctgct acacttttat taattttgta tagtattatt
     7321 ttgataacta ttttgaaata gagagcatta atttttctag tccacaggag ttttctgttt
     7381 ttgtgctgtt aaatataagc acagaaaaag gcagatatat acctgtggac tttttttata
     7441 taaattatga aatatggagg tagttaaaat gagtataatt gaacttaatg aactgactaa
     7501 atcttataga agatttgaga aaaaggcagg atttatgggg agtgtaaagt ctttatttaa
     7561 taggaaatat acagaaaaaa cagctatatc gtcatttgat ttaaagatag aagaaggaga
     7621 atttgttgga ttaataggtc aaaatggagc tggcaaaact actcttatta agatgttaac
     7681 tggtattatt gcaccgtctt ctggagaaat atctgttatg ggttattacc ctaacaaatt
     7741 agaaaataat tttaaaaaac aatatgctgt tgttatgggc caaaaaagtc aactattctt
     7801 tgaattgaca ccagcagata cgtttctttt atttaaagaa ttatatgata tatctaatga
     7861 agaatataaa aaaaacttgg attactttat cgaattattt gaggtatctg actatttaaa
     7921 tgttcaagtt aggacgcttt ctttaggaga aagaatgaaa atggaattaa tagtagccct
     7981 tctacataat ccaaaaatat tatttttgga tgaaccaact ataggcttag atgctatagc
     8041 ccaaaaacaa attaggtatt tcctaaaaga agtaaatgaa acaaaaggaa ctactattat
     8101 acttacttct cattacatgg aagatattaa gagtctctgt agtagaagta ttgttgtaga
     8161 aaatagccgt aaaatatatg atggttctac agagaaacta tttaatcatt ttcaaaaata
     8221 taaaaaaata acagttagtt tttccaatga aactaacatt gtatttccaa aagattgtaa
     8281 agttgttgaa caaacaaatc ataagacatc agtaattgtg ctaaaagaaa gaggaaatga
     8341 aacgattaaa tggatttttg ataaatatga tgttaaagat gtaatgattg aagaagaaga
     8401 tataggtagt attgtagaga gaatatataa gaaaggaagt gatgagtatt atgagcaact
     8461 ataatgaagc aagttcaaat tattcaatgt tttataaatt taaacaaagt attagaaggt
     8521 attatgaagt agccaaaatt aactttaaag tgcaacttat ctggagattt gatgtggtgg
     8581 ttaatatggt gtttacgatt gtaaaaattt tatttgctta tattttatgg aaagcaattt
     8641 ttggtgaaaa taaaatagtc tctggtttta catttaactc tatgttgtct tactatataa
     8701 taagctcatt tttagcaggt attgatatgt caaagagtac cagtgaagag atatgtaatc
     8761 gtgtaaaatt gggcacattt tcaaagtaca tgattattcc aattaatgtt caaaattatt
     8821 ttattgctaa aaattttgga atgacatgtt tttattttgt atttaatatg atttctgcat
     8881 ttgtatggat ttttatattt aaaatagatt ttgcctttac atcaaatcca gttcaaatat
     8941 taatttcaat tttgatgatt tttctagggt ttatttttat gatgaaactc cacttcttta
     9001 ttgggatttt gtcatttaaa tttcttgaga ttagtttttt tagaatgata attgaaaata
     9061 ttgttgaatt tattacagga acaatgatac cacttgtact cttaccagaa actatagtaa
     9121 cagttatgaa ggtatttcca ttttattata caacatattt gccatctatg ttactaatag
     9181 gtagaaacag caatgaggca atacaaggat taattattct agcattatgg gttttatttt
     9241 tttctgtagt aaataaagtt atgtataaaa gaatgagaac attatttgat ggggtgggta
     9301 tatgatgaaa aaaaatttga aaataatatt tatgttatta aaaatgaaat tatcaaagat
     9361 gatggtattt agatttgatt tttttggagc attttttgta gatagttcat tatttatatt
     9421 acaacttcta atgtttaata gtatatattc tcatgtagac tcaatagggg gatggcaaca
     9481 aggagagatg ttaatattta taggaacttt ctcattaata aatgcgataa atatgacgat
     9541 tttctttttt ggtatttatg atattccacg aaaaatccaa gaaggagaat tagactacta
     9601 tattacaaaa ccattagacc cactatttag gttaacattt gaaaatatta atattggttc
     9661 atcaccatta attctagcaa gtatacttat tgttttatat ggaatatcta agttaaactc
     9721 agagataact ttagttatga taataatata tattttcatg gtgttattga tgacattact
     9781 tttttatgat gtatgtatca tacttagaac agtttcattt tttgttatat catcttctgc
     9841 gataatgaga ttagaagata atttattgcc aatgaatatg aaaattcctg gtgtaatata
     9901 tgaaggtgtt tttaaagtat tattctattt aatcttacca tatggaatta tgtcgacaat
     9961 tccgactcaa attttggctg ggacaataac aattaatgga ttaatatact caattttaat
    10021 tgtatttttg tttacattgt ttatgtggag attttggaag ttaggtatga gacattataa
    10081 aagtgcaagt agttaataaa ttagtaaaaa tagacaatgt ataactatat ataaatacgt
    10141 ttcatatgac actaaactat gtgaacaagt atttgcgcta aaggcactga aagctataaa
    10201 atgattaaag taaggattta aaagtttctt tagtagaaat ataattgtgt aaaaaagcaa
    10261 gattaattaa aactatatcc aaataattag atataattct tatgcagaaa ggaggtctgt
    10321 aaatgtctaa ttgtttggaa tatattttaa aaataattaa tgtacagaaa aataaacaaa
    10381 atgagtgatg tatattattc agaaaatcat gaacaagata atctttatga gcaagataat
    10441 ttactggcac aaagatttaa tttatttact ctattgaagt ttgcatttcc tactattttt
    10501 atgatgattt ttatggggtt gtatacaatc gtagatacta tatttgtatc aagatttatt
    10561 aatacaaatg ctctatcagc aattaatatt gtatgtccaa ttattaatct tatagttgga
    10621 attggaacta tgcttgccac tggaggaagt gctattgtag cacgtaagat gggaaatggt
    10681 gatattgagg gagcaaataa agctttttct ttaatcgtat tatcaggagt tgtaattggg
    10741 gtaataatta ctggtatagg tattgtattt atggattata tcattatatg gttaggagca
    10801 agtgaagttt taattaaata ttgtcgttca tatcttttta ttatcttggt ttttgcttct
    10861 gcaaatatac tacaagtatt atttcaatgt ttatttgtaa cagctggaaa gcctcaattt
    10921 ggattatggc tagtgttagg tgctgggtgt gctaatataa ttcttgacta tgtgttcatt
    10981 gttctgttac aaatgggtat tagtggagca gcacttgcaa ctggagtagg atacctaatt
    11041 cctaccgttt caggtatggt tttcttttca aaaaataaag agttacttaa gttttgtata
    11101 cccagactag ataaaagaat attatttgaa gcttgtttta atggttcatc tgaaatggta
    11161 ggtcaactat caacagcagt tactactttt ttatttaatg ctatgatgat gaaattaatt
    11221 ggtgaggatg gagttgcagc tattacaata attatttatt ctcaatttct tttaacttcg
    11281 ctttatatag gtttctcaat gggtgttgca ccaataataa gttataacta tggaagtgaa
    11341 aataatacac agttaaatca tgtttttaag atttgtatat tatttatttc agtggcatct
    11401 ttattggtat ttatcttttc tatatcatgt tcaccaatca ttgtagagat tttttctcaa
    11461 agaggaagcc gagtttatga aattgcaagg tctggatttt atatattttc ttttagtttt
    11521 attttttgtg ggtataatat ttttacttcg tctatgttta cagcattgtc aaatggcaag
    11581 gtctctgcaa tattatcttt tctacgtaca tttggattta taactatagg tttattgata
    11641 ctgccaaata tattaaatat aaatgggata tggttagcag taccaagtgc tgagatttta
    11701 actttatttt taaccatttt ttgtatatgt aagtataaga atagatataa atatatgtag
    11761 atattaaata aatagtttag gtagatattt tatgttttaa taagtaattc aattagtggt
    11821 agatatatac tattgaaaat taaagttata attgatataa agaattaata aaaataacat
    11881 ataaaagttt aaaagggagt tgaacttaaa gtttgattag aaaaagtcaa ttttaagaca
    11941 actcctttta ttttactatt gtattaatct gtatctgtat ctgtaagtgt attagtctaa
    12001 tattataaac tttggatgaa attaattttc tataggtttt aatgtatcat acactttatt
    12061 ttaaagaaac ttatcttaat tttcatagtg attgaatttg tctattagag atgttactat
    12121 tagtaatgta aaagcaagag tagtgaatat aaaaaaacaa gatattgata ctgcaagatt
    12181 agaatataat aaatttttat ggagagattt caatatggat acatatttta ataaaaaaat
    12241 attacttgtt gatgatgaaa aagatatagt agatttaatt gaagaagtac tcataaatga
    12301 tggttttaaa aacattatta aagcttataa tggtttagat gctatttcgc tgtgtaagat
    12361 agcatgtcca gatgttgtca ttcttgatat aatgttacca gatatagatg gtatagaagt
    12421 gtgtaaaaaa attcgagagt tttcatactg ttctattctt tttttatcat caaaaaatga
    12481 tgatattgac aaaatactag gtcttagtag tgggggagat gactatataa caaagccatt
    12541 cagtccaaga gaaattgctt ttcgagttaa agcgcaatta cgtagacaac agtatcaaag
    12601 cattgtacca tcagatagtg cagttattaa aataggagat attactattg atatagaagg
    12661 aaaccgtgta tataaagata gaaatgagat tgagttaact ggaagggagt atcatctttt
    12721 aagctacatg gctcaaaatg taaataaaat tataggaaaa gaaagactct atgaacaagt
    12781 atggggagta tatagtagta tttgtgacaa tacaattatg gtgcatatac gacatataag
    12841 agaaaaaatt gaagataacc cctcaaatcc taaaatactt ataacggtta aaggactagg
    12901 atataaactt gtgaatagaa tcgattagaa tagattttaa tattaatgaa gatgtctcaa
    12961 gttgaatttt tagttaatga ggcattcttt tatgtgtgaa gtcaaatgta ttttcgttat
    13021 ctcaacaatg aagaagaggt ctatctctat tttgagatag cctcattaag tttatatcct
    13081 ggtaatgtac tttatgttct aataaaattt ttttagttta tttgcagtga aacattaact
    13141 agaacattaa aatttgcatt gaaatatcaa ttaaataata ttgtatattg tgtataaact
    13201 tatactaatt gtttttctat attaaacact ttatcaatcc aacctttata aaatttttct
    13261 tcatgggaaa ctaggattat acttcctcta aactgtttca aagcattttg taaagcatcc
    13321 tttgtctctg catcaaagtg attggttggt tcatccagaa taagaaaatt acaaggagat
    13381 aacatcatac aacataattt aaccttagat tgttcaccac cacttaaagt agatacactc
    13441 cttgaaacat gctcttcctt cacaccacat ctggctagat gacgtctaat ctcttttgta
    13501 tttaattttg gatatttatc tgctactatt tgaagtgggg ttttatcagg attttcccat
    13561 tttaaatctt gctcataata accaatttta acttgttctg aaaactggaa atctccagat
    13621 atacgaggaa tatctttgac taatgtcttt aacaaggtag atttaccgat tccattaaaa
    13681 ccagttataa ctattttttg acctccatca acagaaaaat ttaattttgg aagtaaagaa
    13741 tagtagtatc caacttcaag atttgttata gtaagagcat tttgagccga tatttcaatt
    13801 tctgaaaatt gaatatttgg ttttcctgta aaactaggag gagctattct ttctatttta
    13861 tccaattgtt tttgtcttcc tcttgcaatt tttgaattta ctccagcttt atttttacgt
    13921 ataaatgtct cttctttttc tattttcttt tgttgtgctt gataccttct aatataatct
    13981 tctcttaaat gtgatttttg tttaaggaac tctgaatatt ttccatgata ttttttaatc
    14041 ataccaaatt ctatatcaag aatacctgta gaaatttttt ctaaaaaatc aaaatcgtgg
    14101 gaaactacaa taaatgcccc attaaatgta tttaaataat ttgagagcca ttcaacatgt
    14161 tctttatcta aaaagtttgt tggttcatct aataatagaa catcatggtt agaaagtagc
    14221 aattttgcta aaattacctt tgccctttgc ccaccactaa gctcttcaac aacacgattc
    14281 attcctattg aatctaatcc aagaccattt gctattttat ttatttcatt atctatagaa
    14341 tagaaattac ttgctattag ttgttcctga taatctgatg ctttgattag atattgctca
    14401 ttttcagata tagcactttt ttgatagagt aaattcattt ccttttctat tttataaagt
    14461 tcttcaaatg cagtgtgtaa atagagtgat attgttgtat ctctattgat ttctgcatat
    14521 tggtctaaat ggcctatatt gatattaggt tgccacttga tttctcctga atctggaaca
    14581 acttctccca ataaaatttt aattaatgta cttttacctg taccattttg gccaactaca
    14641 cctatatgtt cacctttgaa taaatcaaag ttagctttct catacagtga tttatccata
    14701 aaacattgtg ataaattagt tacttttaat aaactcataa ttcaaactcc ttttctttta
    14761 ttaaaaaatt tatggaaata aaaaaagcag aggaatggca ttgtaaaatg ccgacctctg
    14821 cttttggaaa aacaaaaggt catagacaag cattgttgtc tatgaccaga aaatctatac
    14881 tatctatcgg caaataggta attttaaata agcatagcca aaaaactata ctaaacctat
    14941 atttgccgat gaagatagaa agctttaagc tttcagatta acctctacac aacgcttcat
    15001 cgtatacatt tgtgtagagt taagttttct aacaagttta actaattcta tataaatcat
    15061 aattattcac atcctaattt tagtattaat tctagtttat ctatatttaa attgtacttt
    15121 tgtactttgt ggattatctt atttatttaa aatatgtttt attaattata gcttaagtta
    15181 tgttatcata atacttggtt atagtcaata agaaagttat atttgctata tattatatta
    15241 atttagtaaa tctatcatta caaagttatt tgaagactcg ctaccaataa aattttatag
    15301 tctatgggaa aattaagatt atgagtttta tacttttgat atagggatac tattttcata
    15361 aatattacaa catgtagaag tatatgtcta gtattgttga attttcaatg atatattaag
    15421 gttctaatat attatacaaa ataaaaacat taattcaact taaataattt taagtattat
    15481 atgtgttttg attaaagaaa actaaaattt taaggaaaaa taacatattt gtattaaatt
    15541 ttattgaaat gattaaattt atttacttaa tgtaaattat tagctaaaat tgtattaaat
    15601 aattaaggag gtgagtagct ctggatgaaa tagatttaaa aataattaat tctctaaaaa
    15661 agaatagtag ggcttcaacc tctgaaataa gcagacaaat taatttatca gtaccagcag
    15721 tctcagagcg tataaggaaa attgagcagt caaatttaat tcaaaagtac actattcaaa
    15781 ttaatcggga aaaaacaaaa tataagcttt tggcttttat atttatcaat actgatttat
    15841 ctaaaagtat gaaagaattt aagaaaacta tattggaata tgatactgta ttagaatgtc
    15901 atcatatttc aggagagtat gattacttaa taaaagtatt agcagaagat acaagtaatc
    15961 ttgaagagtt tatatcagtg actttgaagg aaattggggg gattcaaaaa gctaatacaa
    16021 ccattgtttt atctacaatt aaagaagaat ttaatacaat aaaagatatt ttttaaataa
    16081 tttactagta aagtgttaat tatattttag gaggaaaata gaatatgaaa gttttagttt
    16141 ttttagcaaa aggttttgaa actatggaat ttagtgtatt tgtagatgtt atgggatggg
    16201 ctcgtaacga ttatgggcat gatattgatg tagtgacttg tggatttaaa aaacaagtta
    16261 tgagtacatt taatatacaa gtattagttg ataagacgat agaagaagta tgtgttgatg
    16321 attatgatgc cctagctatt cctggaggat ttgaagaatt tggattttat gatgaagcat
    16381 atgattcatc ttttttaaat ctaataaggg agtttaattc aaaagaaaaa ataatcgctt
    16441 ctatttgtgt ggcagcattg ccagttggaa aaagtggtgt tttaaaaaat cgtaaagcta
    16501 ctacatatca tttgaaaaat ggtaaaagac aaagacagtt aagtgagttt gatgtaaatg
    16561 tagttaatga accaattgtt gtagataaaa atattataac ctcatattgt ccagaaacag
    16621 ctccacatgt agcttttaag cttttagaaa tgttgacatc tagagaacaa atggatgaag
    16681 ttaaattagc aatgggattt aaactgtaat gctctataga ctttctaagt aatcagaacc
    16741 ccagtcacac atggaatcta aaattggaat aatactcttt ccaaaatcag atagcgagta
    16801 ttctactttt ggaggaacaa caggataaac cactcttata attaaaccat ctgattctaa
    16861 ttcacgtaat tgttgagtta acatcttagg cgttgcttta ggaattaatt tttttaattc
    16921 attaaacctc aatatagtgt ctttaagatg ccaaagtata agtgtcttat atttaccacc
    16981 aatcaatgca agtgttgctt ctattgggca actgtaatta tctaaacatt ttttcatttt
    17041 tgagctcctt tatttatttt aaagtatctt tttagatact atattacaaa aaagtgcatt
    17101 attgtaatat agtaccatag tgataaaatt atagcatagg ttaaataaat catcaactta
    17161 gcctaataaa acaatagggg gtaaataaat gaatttcgtt gaacttacaa aaaaaagata
    17221 ttcttgccgt aattatcaag atagaaaggt tgaaaaagaa aaacttgaga aggttttaga
    17281 tgttgcaaga atagctccaa ctggtggtaa tcgtcaacct caaagattga ttgttattca
    17341 ggaaaaagaa ggtataaata aactttctaa ggctgccaat atatatgatg ctccattagc
    17401 aattcttgta tgtggagata aagacaaagt ttggacaaga ccttttgatg ggagacaact
    17461 tacagatatt gacacaagta tagttacaga ccatatgatg ttacaggcaa ctgaacttgg
    17521 attggcaagt gtttgggtgt gctattttaa tccagatata attagagaag agtttagttt
    17581 gccagataat ctagaaccaa taaatatatt attgatggga tatgaatcta agataccaga
    17641 gagcccagag agacatgaaa aaactcgtgt tcctttaagt gaaattgttt cttatgaaac
    17701 tctttaatta taaaattaag ttattcatag gataaatgta ataaagtgtt tattataaac
    17761 aaaattcaaa ataaataata tctacatata attaatttta gtgaagtata tctagtttaa
    17821 ctagaatgta tacttcacta atttatttat tcaaaatatt tatgaggatt ttaatatatt
    17881 aactaaaata tatcaattga attaactttt aaatctggaa aagcacttga aatatagtgt
    17941 tttatgtcat ttaaatggtc ttgaatttgc ttttctgata ttttatcagg tccatataca
    18001 aaatacataa tatttttagt atcttttgtt atagctgttc gattatcagg gccattaaga
    18061 atactagata atataccaat attatctgat aaaaataaat catcttttgt taaaatatgc
    18121 tctttaccac ctataccata aaaatgttca cttccatttg ctaattctat tttcaaaggt
    18181 aattccattt tatcaaggtc atgacctgct gttagaagaa gatttttcaa ttctgctaaa
    18241 aacattgact caattgttac acctacatct gggatacctt ttgattttaa tagaatagat
    18301 tctaattgaa gcaatacagg gtaagttttc ttgaattttt tataatactt tatataactt
    18361 gaaacaggct ctgttttaat tttttcttta cgattgtatt ctgggtgttg ttctataata
    18421 tttttaattg tagagttttt aagaactaaa aaattttctt tattaggttt tgaatgtgta
    18481 atatttataa ccataactcc aaatttaata ttgggataaa tttctttaac tttatgtgat
    18541 atttctaaca taatatagac tcctttttat tttatatttt atgatataat cttagcaagt
    18601 taaaagtgga ttttgtagtt ccatttttat agtaatttta tagaaccact tttggaggta
    18661 ttagaatgtg gattacgatt aatagaaata gtaatatatc attatcaaga caaatttatt
    18721 gtgagataaa agatatgatt ctggatggaa gcttaaagtt tgagcaaaaa ctgccatcat
    18781 ctaggacttt atcaaaagaa ctatctgtat ctcgtaatac agttttagat gcatataatc
    18841 aattaattgc agaaggatat ttggaaacaa ggaatggata tggaacaatt gtagctaaag
    18901 gaatatcagg gcataaagtt gattttaagt taaatgatac ttttagagat gaaaaagatt
    18961 tacatactaa taaacaaaaa aattatatag attttcgttc aggagttcca gaattaaaac
    19021 tatttccacg caaagaactt tcaaagctat ataaacaaat actgtatgat ttatcagatt
    19081 cggatttcag atatggaagt acagctggtg tatgggtttt aagagtggaa atatctaagt
    19141 atttatttcg tactagagga attagatgta attccaaaaa cattatgatt gtttctggtt
    19201 caacacaagg tttatcttta atttcacagc ttttatacaa aagtaataaa aaggttgctg
    19261 tagaagaccc tattcataaa ggcttattaa aggttatttc atctattgga tattcagttg
    19321 ttggaattaa agtagatgat aagggaatga ataccaactt aataaaatca ataaagggga
    19381 tttcttttgt ttatactaca ccttctcatc aatatccatt aggagggatt ttacctattc
    19441 aaagaagact agagcttatt aaatgtgcaa cagagaatga ttactatata attgaagatg
    19501 attatgatag tgaatttaga tatgagggtc aaccaattag ttctctttat gaacttagcc
    19561 caaataaagt aatttacata ggttctttta gtaaaatatt atctccagct attcgtgttg
    19621 gatttatgtt attaccagac aatattttag aaagctataa aaccttaaaa atgtactctg
    19681 atgttcatac agaaacaata ttgcaatatg tattagctga atttattgga aatggaggtt
    19741 tagagaagta catatttaga atgaagaagt tgtacaatag aaaacgtaaa tatctcataa
    19801 aagttttaaa cacggaattt aaaaatgaat ttaaaataaa aggtgaagca gcagggcttc
    19861 atatagttgc tcatttctat aatgttaaat ttacggaaag tttaattcat aaaatttttg
    19921 aagcaggtat aaaagtttat cctatacaac aatatgcaat tgagaatata ttagaatatg
    19981 accatgaaat tatactaggt tatgctcatc ttagttttga tgaaattgaa aaaggtgtaa
    20041 aaatactcag caaaataata catatgaatc aaggtacata gataaaacat gtagcaatct
    20101 acatataata gatgtaatta agtttaaact ttaaaagaaa tatatattaa tatttttgta
    20161 taaaaatact tgatattgtt ttatcgatac tttattgcta tacactaaat taagatagca
    20221 atttatatta agaaaaaact ttttgcatag gttataatac ttaaaaggag aggatagagt
    20281 tgaaaacaaa tacagatgtt attatggaga taattgatta tatagaagaa aatcttacag
    20341 aaaatctaaa tttagataaa atagcttata ttgctaatta ttcaaaatac catttacaca
    20401 gaatgtttac aaatgttgta ggatgtacta tccatcaata catacagagg aggagactta
    20461 ctgaagcagc tagacaactt atatttacag acaaatatat tatagatata gctattacag
    20521 taggatattc tacacatcaa tcttttacac tagcatttag aaatttatat aaagaatctc
    20581 cacaagtata tagaaataaa gatgaatttt atcctattca acagagattt gttttaaatc
    20641 taaatcatca aattcaaagc gaaaaatgta tttcttatgc aaatatgaat tataaacaaa
    20701 gaagagatga aatcaattct tatcttaact ttaatattaa ttgtgaggtg aaagtagcat
    20761 gagtttagtg ccacatgtaa ttgaacaaac aggtcaaggg gaaaggtctt atgatatata
    20821 ttcaaaatta ttaaaagata gaattatatt tataggagaa gaaattaatg atgctattgc
    20881 aagtttagta gtagctcaat tactgttttt agaatcagaa gacccagatt cagatataat
    20941 tatatatata aatagccctg gtggctctgc tacatcagga tttgctatat atgatactat
    21001 gaattatgtc agatgtgatg tatcgactat ctgtattggt atggctgcaa gtatgagtgc
    21061 ttttttactt gcaggaggaa cacatggaaa aagatgtgct ttacccaatt cagagattat
    21121 gattcatcag ccaatgggag gagctaaagg tcaagcaaca gatgtaaaaa tagctgtaga
    21181 taatatattg aaaataaaag aacgattaga taaaattctt tctgaaaata caggtaaatc
    21241 aatagaagat attagaagag atacagatag agataatttt atgactgcac tagaggctaa
    21301 agagtacggg ctgattgatt atataatgaa taggaatgaa taggaatgaa taaagtatca
    21361 aagtatcata ttaaaatgtc tgttttacaa atatatatat aatattaaat aagagtgtct
    21421 caaaatgaac tttttagttt atgggacact cttttttgta tgaaattaaa tatggtttta
    21481 tcatttcaac aattattacc aaatttcaag tttaataaat tctactataa aaaatacata
    21541 aaagataagg ttctttagta actttctgtc gtcaaaagtt tttataaatt taataacaaa
    21601 acaagagaaa ataccatata ttacttatgt ctgcaatata tagaagacaa aggagttgta
    21661 cattatgatt gcaataagtt tgtgcatgat tgttaagaat gaggaagaag ttataggcag
    21721 atgcttagaa tgtgtaaagg atatagtaga cgaaattata attgtagata ccggttctac
    21781 agattccaca aaagaaatag tctcaaaata tacagacatg gtgtacgatt tcgaatggat
    21841 agatgatttc tctgctgcaa gaaatttttc attttctaaa gcttctaaag actatactat
    21901 gtggcttgat gcagatgaca taattttaga agctgataga gaaaaattat taaagattaa
    21961 agaaagcttg gatacttcca ttgatattgt tatggcaaaa tacaatgtat cctttgatga
    22021 aaatggtaac ccaactctct catattatag agaacgattg tttaaaagaa gttctaatca
    22081 taaatgggtt gaccctattc atgaagtagt acctcttttt ggagaggtat tttattctga
    22141 cattgcaata tctcataaaa agcttcatag acaggaccct ctaagaaatc ttagaatttt
    22201 tgaaaaaatg atttcagaag gaaaaacatt ggagccaaga caccaattct attactctag
    22261 agagttgtac tataatgcta ggtataaaga agcaatagaa ggttttacta agtttttaga
    22321 ctcatcaaga ggatggattg aggattgtat cagtgcctgc agagatttag ccacatgtta
    22381 ttacttaatt aatgatgaaa aaagtgcctt atattcattg tttagaagtt tcgaatttga
    22441 tgagccaaga gcagaaattt gttgtgacat aggaaagcat atgtttgata gacaaaaata
    22501 taaagaagcc attttttggt ataaggtagc attaacaaga gataagaatg atactaatgg
    22561 aggttttaag tcaaatgatt gttatggata tataccatct atacaattaa gtgtctgtta
    22621 tgacaggtta ggagaatcag ataaagctat ttattatcat gaaaaaacaa aagaaatcaa
    22681 acctaacgat agtgctgttt tgcacaatga aaattatttt agtaaattta aaaatagttg
    22741 ataggtcaca atcatactaa atttgacata ttatatatag agcttaaaag ctcaaatata
    22801 tcaggaggtg cttttaataa tgagtagaaa taaatatttt ggaccatttg atgataatga
    22861 ttacaacaat ggctatgata aatatgatga ttgtaataat ggtcgtgatg attataatag
    22921 ctgtgattgc catcattgct gtccaccatc atgtgtaggt ccaacaggcc caatgggtcc
    22981 aagaggtaga accggcccaa caggaccaac gggtccaaca ggtccaggag tagggggaac
    23041 aggcccaaca ggaccaaccg gtccgactgg cccaacagga aatacaggga atacaggagc
    23101 aacaggatta agaggtccaa caggagcaac agggggaaca ggcccaacag gagcgacagg
    23161 agctataggg tttggagtaa caggcccaac aggcccaaca ggagcgacag gagcaacagg
    23221 agcagatgga gtaacaggtc caacaggtcc aacgggagca acaggagcag atggaataac
    23281 aggtccaaca ggagcaacag gggcaacagg atttggagta acaggtccaa caggcccaac
    23341 aggagcaaca ggagtaggag taacaggagc aacaggatta ataggtccaa caggagcgac
    23401 aggaacacct ggagcaacag gtccaacagg ggcaatagga gcaacaggaa taggaataac
    23461 aggtccaaca ggagcaacag gagcaacagg ggcagatgga gcaacaggag taacaggccc
    23521 aacaggccca acaggggcaa caggagcaga tggagtaaca ggcccaacag gagcaacagg
    23581 agcaacagga ataggaataa caggcccaac aggggcaaca ggagcaacag gaataggaat
    23641 aacaggagca acagggttaa taggtccaac cggagcaacc ggagcaaccg gagcaacagg
    23701 cccaacagga gtaacagggg caacaggagc agcaggacta ataggaccaa ccggggcaac
    23761 aggagtaacc ggagcagatg gagcaacagg agcgacaggg gcaaccggag caacaggtcc
    23821 aacaggagca gatggattag taggtccaac aggagcaaca ggggcaacag gagcagatgg
    23881 attagtaggc ccaacaggtc caacaggggc aaccggagta ggaataactg gagcaaccgg
    23941 agcaacagga gcgacaggtc caacaggagc agatggatta gtaggtccaa ccggagcgac
    24001 gggagcaaca ggagcagatg gagtagcagg tccaaccgga gcaacagggg caacaggaaa
    24061 tacaggagca gatggagcaa caggtccaac aggggcaaca ggtccaacag gagcagacgg
    24121 attagtaggt ccaacaggag caaccggagc aacaggatta gcaggagcaa ccggagcaac
    24181 aggcccaata ggagcaacag gtccaacagg agcagatgga gcaacagggg caaccggagc
    24241 aacaggtcca acaggggcag atggattagt aggtccaacc ggagcaacgg gagcaacagg
    24301 ggcaacaggt ccaacaggcc caacaggtgc tagtgcaata ataccttttg catcaggtat
    24361 accactatca cttacaacta tagctggagg attagtaggt acaccaggtt ttgttggctt
    24421 tggtagttca gctccaggat taagtatagt tggtggagta atagacctta caaacgcagc
    24481 agggacattg actaactttg cattttcaat gccaagagat ggaacaataa catctatttc
    24541 agcatacttc agtacaacag cagcactttc acttgttggt tcaacaatta caattacagc
    24601 aacactttac caatctactg caccaaataa ctcatttaca gctgtaccag gagcgacagt
    24661 tacactagct ccaccactta caggtatatt atcagttggt tcaatttcta gtggaattgt
    24721 aacaggatta aatatagcag caacagcaga aactcgattc ttactagtat ttactgcaac
    24781 agcttcaggt ctttcattag ttaatactgt agcaggatat gcaagtgcag gaattgcaat
    24841 aaattagatt agataagaaa aggcttctaa tatcgtattg atattaggag cttttttaag
    24901 aatgattgat gaacatagtt ttaatattaa ttagtgcgca tattataaaa tactattata
    24961 taatataaaa taataacatt aaattggaaa tatagaatta tctaggggtg atactatgaa
    25021 aaataagatt gaaacatcaa tgaacaactt tgtaaaaaat tatcataaat caaataatat
    25081 aactacaaag tggcaagaac ttttgtttgc ttatgcatct gctgaagatg atatgtttca
    25141 gaagctaaaa agtgttgtag gaacatctca tgctatgcca aaagattttc tggaaaatgg
    25201 aaaaactata attacatatt ttataccttt tgatgaaagt gtaaataata gtaatattgg
    25261 agggtttgaa tgttctgaaa tatgggcaag agcgtacata gaaactaata tgttgatttc
    25321 aaatttaaat gatttcttat gtgaagaact agagaagatg ggggctaatt catatgctat
    25381 tcctgggtcg tataattttg acagtgaaaa attggtaagt gactggtcac aaagacatgt
    25441 agcattcata gcaggtcttg gaacatttgg tttaaataat atgcttatta cagaaaaagg
    25501 gtgttgtgga agaataggaa gcataatcac aagtttagaa ttagagccaa ctaaaagacc
    25561 agaaaatgaa tactgcttgt ataaacataa taatagttgc aaaaaatgtg tcaacaaatg
    25621 cgtgaaagaa gctttaaaag aagatggatt taataggaat aagtgttatg aaatgtgtat
    25681 gataaatgaa aaaaaataca cttatatagg tggttgtgat atttgtggaa agtgtatagt
    25741 tggggttcct tgttcggtaa taaatccctc aagataaagg acttatgttg attaataact
    25801 ttaaatatat ctaataagaa gaaaaaagtt agtctctaaa atagatgaat tatgaaaata
    25861 aataaagata atataaaggt tagatgaaga agttatgtta attgacaact atttatactg
    25921 ctgatagact tatatgtatc gttgcaatat ataaggaggg gtgagattta tgtttgattt
    25981 aatctttatt tttgcatagc aagggctatt gcaaatataa aatttaatat ataaatagcc
    26041 ctttgctatt cctaataaaa aattattatt taataggagt gcagaatgag ctataaaaaa
    26101 gcaatacata tattaccaga agaattactt gaattgatac aggaatatgt agatggagaa
    26161 tatatctata ttcctagaaa gtctaataat aaaaaagaat ggggaagtag tacgtctact
    26221 cgtgaagatt tatcaattag aaatatgcaa atttatgaag attatcaagt tggatatgat
    26281 ttgaaatatt tatcagaaaa atattacttg tctttgaaaa gtattcaaag aattgtatta
    26341 caagaaaaaa gaaaagatag ttaagaatga gccagtatag ttttataatt atactggctc
    26401 attttctttt gataaaagtg tttgaggtat agtttcttat aagtggatgc tataatttaa
    26461 ataatttcaa taacttgtac agcagataac gaatagagtt gtaaagtatc taataagaag
    26521 tagtagcttt ttatatgttt gaaattaaaa tttattaaaa ttaggaggtc ttgttttgaa
    26581 aaaaatactt gattatattc caccttttca taactttcta ttaatttagt aatcttatag
    26641 aaaggaagtt gcatttatga aaaaaattac ttgggaggtg gaatatatct cccagttacc
    26701 tgtaattagg tattgtaaga aatgtggaaa aaagactgaa tatatttgtt caggtttatt
    26761 tagggttaat gctcaacata aatatttgga tatatggtta atatacaagt gttcaaattg
    26821 tgattcaaca tggaactcaa ctatatattc tcgtgtcaat ccaaaaagct taaatccaga
    26881 aacactagaa caattccata caaacgatta taatcttgct aagcaatatg ctatgaatac
    26941 ggaactttta catagaaatg gagcagaagt tggtattcca gagtataaaa tagtaggaca
    27001 agaaattaat attaatgacc ttacacaagt tcatattatt agtaaatatc catgtcagtt
    27061 aaaggtatca acattattac gtgagaaact aggtttatct aggaaggtat ttgacgaaat
    27121 gttggattgt gggattatta gaagtaactc aggagttgat ttaaagaaag gtaaattaaa
    27181 ttatgaaatt atgttagata taggcaagga gaagcaaaag tcttcaaatt gattagaata
    27241 actaacttaa tatattttca ggaacatcat ttaaaaaaat agtggcttta aaaaatttaa
    27301 agccactatt ttttaatatg ttttatatga atatttaaaa tatagtgtgt attcttcaaa
    27361 attatagcga taattaaaat attactataa aaaggaaggt gcatactttt tgtttaacaa
    27421 aaactagata ctaaaaactt ctcgaagaat atgttattaa aatcgctaaa tatattcaat
    27481 atatattaaa aaattgtatt ataatgaaaa ttaaaataga ttgtaaatcc aaaaagtaat
    27541 acttaatgtc caaaaagcta aaattaaaat ctaaatagct atttgctaca gaagtatgtt
    27601 aaaatatgta aaatgaatag aaattttata tatttattaa aaatatataa gaaaggatgt
    27661 ttaagaattg tgattagtat aggaatatgt gatgatgaac tacattatcg aataaaaata
    27721 aaagatatat taagtgaaat attaagttca tatcctataa attataacat atatgaattt
    27781 tcatcaggag aagagttact aaataactat cctaaagatt tagacatatt aattatggac
    27841 atacaaatga aaacaataaa tggtatggat actgctagga agataagaga gtttgatcat
    27901 aagttagaaa taatatttgt tacttcattt gtagagttta tgcaagaagg ttatgaggta
    27961 aaagcataca gatatatact aaagccaata aataaagaaa aaatttctaa aagtgtatta
    28021 ccttgtataa atgagatgat gaaaaagaga aataattact taactataaa tgtaaaaaat
    28081 tatgtagacc gaattaaaat agattctatt acttatatag aaacagatag accgaatata
    28141 ttgatttata cacatgatga tatgtatatt actaaaatga gtatatctaa aatagaaaag
    28201 atattaaatg aatatggatt ttttagatgt cataatagct atatagttaa tttaaaatta
    28261 gtagaatcaa tgagtggaag tactgttata gtagatggca aaagtattcc aataagtaaa
    28321 tatagagtta aaggcttgaa acttgctata actaatatat taggggatat agtatgctaa
    28381 caaatctaag tataggattt tggaatatta ttaatttagt ttcaggtact atagattgga
    28441 ttatattttt tttagtaatc cacttagttg gaaagaggaa aatatctaat ataagatata
    28501 tatttacaac aataattata tttatattta tggcatacat aaatgtagtt aaagtgtttc
    28561 caaatattaa gattcttcta tgtatggtaa taggaatatc attttatata acatcatatg
    28621 aagataaaat atataaaagt ataatagtta gtttattgtt ttggctagga ttaatgattg
    28681 ctgaaggttc tgcagtaggt ctagtagtat ttataaataa attaaataat attaacataa
    28741 cacaacatga aaacttattt agaatacaag ctattattat gtcaaaggta tttttattta
    28801 taggttttat attgtttaag tattttaaat tatctttaga ttttaaacct aaagatatga
    28861 tactaatagg aatacctata ttatctaata tagttagctt attacttata tttgaatata
    28921 atttaagagt caatacaatt actacagaaa acttatttat atttatattt gcaattttat
    28981 tgattttatc atcaagtata atacttttaa tagtaatagg gaaaattgta caagacgata
    29041 aattaaatct agagtatgaa cttataaatg aaagaataaa aacaaactac aggaattatg
    29101 aaaatataaa cgaagttcat gatagattaa aatatgttta tcatgatttg aaaaaccata
    29161 tgatttgtat aaaaaattat gacactaaag aagaaattat atcatatata gataacttag
    29221 agtttcagat aagtgaattt gaacatttta aaaatactgg aaataaaaca ttagatttaa
    29281 ttttagcaga aaaaataagt atttgtaaaa agtatgatat tcaaattgaa gataacataa
    29341 atatatctaa attaaacttt attgaaaata atgatatatg ttcaatattt gcaaattcac
    29401 tagataatgc aattgaagca tgtatggata taaataatga gctagaaaaa agaatagaag
    29461 ttaaagctac atatataaat aaatttgcaa taataaaatt tataaatact aaggtaaatg
    29521 atattaaact tattgataaa cgtattcaaa ctagtaagga tgatgataag atacatggaa
    29581 taggacttgc tagtattaaa tatatagtaa ataaatatgg tggtgaaaca atagttaatt
    29641 actcagataa tgagtttata ttaaaaataa tgataccaat aaaaagttag gagagtacaa
    29701 aatgaaaaaa atagcgttaa acttacttaa aaacatctca gcattaagtt ttgggatagc
    29761 tgtattatca gcaaacagtg caagttcttg ggtagcacat caggcaaaag agccacaagc
    29821 tttacaaaaa ctaaaaaaat aacattaata aaaggacctc ttcttattaa aggagaggtt
    29881 ttacatgttg aggaggaaat atgtttaaaa gattatcata taaatttgcc aatatattag
    29941 ttaataatga gattgtagaa agtgaagatt ttgaaatata tagatatgga tttgaaactt
    30001 tgatttattt catagttaat atttcggtag cattatttat aggaattatt tttgatagat
    30061 ttatacatac agttatattt ttaagttgtt attgcacttt aagacagttc acaggaggtt
    30121 atcatgctag gaattataaa gagtgtacat taacatttgc agttatttat ttgataacta
    30181 ttttttcagc taataatata gatataaata aatataagta tttattagta ttattaatga
    30241 ttattagtat actaacaatt tacaagttag ctcctttaga acatagaaac aaacctctta
    30301 gtgaaagtga gaaaaagcat tatagaaaga ctgtgcaaaa aatactattc gtaataatat
    30361 gtttaattat tttatgtaaa atacttaata tttttcagca atatgtgatt tatgcaataa
    30421 tttctatata ttggattgct atattaatat atattggaat gaaggttaat aatgatcagt
    30481 aagattataa aacattttct aaattaatta atttcactaa ttaatattaa ttttaaaaaa
    30541 taatgtcaag gtaaatattt gaacttatta aatatagttg aatctattgt aagttgtatc
    30601 aaaggattgg ctaggagggg cataaatatg agtagtatta aggttatatc agactttata
    30661 accaaaataa ttagaaaaaa tattttattt gcaataacag ttattattat aaattaccat
    30721 ctcatacaac gatggacaca tatatccaat agaggaattt catttaatga ttacttatcc
    30781 ttgagtgtat atacttgtat gacaattagt attttaacca tgttaaatgt aattttttat
    30841 atgagaaaaa gttatgatac tgtaaaaata ttatgtagaa ataattttga ttatttttta
    30901 ggtatttctg ttgctgtaac tagaaacaac tttatatttt gttgcatacc aattatatat
    30961 gctatagcat actcaagttt aataagaaca ttatcaattg taacgattaa atcaactata
    31021 tttatattgg tatttgaatg gtttttacca cttactattt tatctatagt gatatcattt
    31081 atttcagtta ttataaataa tttaatacta aagattacct tatcatcagt atttttatat
    31141 ttaacatcaa ctaagatgtt ggatagagtt attgatatta aagaaccatt ttttaaagca
    31201 ttatttaaat cacttaatat atttgaagat caatcatatg cttactattc tgattttgta
    31261 gggaaagtta ataattcagc atatgttata gataagatta tacctacact atttgcaatt
    31321 actttaataa tagtagcata tctgatatta aatgatatta gaaagaaaca gattaaatca
    31381 ttaatagtta tagtagtgta cagtatttta tttattggat taaattatat atcaatagat
    31441 atgtcaacat atagagatga ggaacgtagt tatggagagt atttaaagaa atctaatatt
    31501 agtgatatta aaataaaatc gcatgatatg aagattgact ttaaaagcaa gtcaaatata
    31561 aaagatagaa ttgttttatt aaacacatca aatgaagagt taacaaatat taatatgatt
    31621 ttagatgaaa tatttgatat aaaatatata aaagtaaatt cggaaaatat aaaatttaac
    31681 aaaaaaaagg atttaattga agttaattta aaggaaagtt tagagcctaa tgacgaggtg
    31741 acattagata tagagtatga aggctatatt aatgtgttga catcttggaa tcaaaataaa
    31801 tatatagcta ctaaagatga tattatgctt ccaccaggtt ctttagcgtg gtacccaaaa
    31861 gtaaatcaat caagttgtat ggcttataaa ataaatttaa gttctaaaaa taatgtctat
    31921 tcaaatttac ctttactgaa taagactagt aattttttaa aaaaagatta tatatttgaa
    31981 ggaaagtcaa atgatattgc aatatatgca ggtgagttta aagaagaggt aaaagatgat
    32041 ataaaaataa tctatccatc agatagtaat gtaaatatgg atatagagga aaaaattaaa
    32101 tttttaattg aaacaagaaa tgaaatatta ggtgatagca aagcatctgt agatgctaaa
    32161 aaatatgtag atgaagtaga taatgttatg gaatcaataa aaagaaaaaa aattaatatg
    32221 atattagttg ctgacttacc tagcaatgaa tttttatatg aagactcaaa gggagagaaa
    32281 attagcataa ctatttatca ttggctttca ggaaatatcc ttataataga taaataataa
    32341 aggaggttaa tatgataaac aagttagaaa taagaaatct aaattttaat tacaaaaata
    32401 aaaaagctct ggataatata aatttaaccc taacagatgg agtagttgca ttattaggtc
    32461 ctaatggtgc tggaaaatca acattaatga ggttattagt gactctttat gaaacttcaa
    32521 ttggagagat agaattaaat aatataaagt atagcaaaaa caatgagaaa atcaaggcaa
    32581 atgtaggata tatacctcaa gattttgata tgtataataa tataaatggg caagaatact
    32641 tagagtttgt agctaaaatg agaggtgtat ctaagaatga tttaaagaaa catatacaaa
    32701 aagtagtatc aaaagttaat ctggacaagt ttataaataa taaaataggt acatactcag
    32761 gtggtgtaaa gagacgacta ggtatagcac aggctttaat aggtgattct aagttaatag
    32821 ttatggatga acctacagtt ggtcttgatc cagagcaaag gaatgaattt agaagattgt
    32881 taccagaaat aagtaaaaat tctatagtat taatttcaac tcacatagta gaagacatac
    32941 aatttaactg tgacaaatta attatattaa atcaaggcaa aatattatat gatggtacaa
    33001 ttaataaatt tattgatatg gttgaggtgt attcagtagt agtttctaat gatgaatttg
    33061 aatatttaga acgtaatatt gatattattg attatagaag agttaaagat ggtgtgcaag
    33121 taaagtatat aggtaaaaat acagagaaac ttaaggatag cttaaggata gatacaactt
    33181 tacaagatgc ttatataaac tttttacata aggagttaaa ataaatgtgt atattggagt
    33241 taataaaatt tgagcttaaa aaaatagagc ttaaacatgt gttaatattt gtaattattt
    33301 ctatattatg catatttatt acaggcatat tagattataa tgagttaata ttttataatg
    33361 atgtacctat aatggataca tattctatag caggatggtt aggaattata aagatatcag
    33421 atgtttttat accaataatg tcagcaatca caataattta tgtgttttca aaagattatt
    33481 tgggtaatat aaatgagata cttactttat ataataaaaa aaaatataat caatttatag
    33541 tatttagatg gtgtgttata ttagctatat atactgtatt attattaatt tcaattacaa
    33601 tagtatatac atttacgtca gtgaaaggtt ttaacataga caaaatagat tttgtttata
    33661 gtgtaaagcc aatagatgtg tttttaaaga catttcctac attattatgg tatacaacat
    33721 ttccactaat gatattaaca ttaacaaaaa atagatttac aacaatagca atattaatta
    33781 catatacatt tgctgatata ttttttattt tacatatgta cccatttgtt agtatgatta
    33841 atgtaaatgg attttatatt caaaagatgt tttatatgcc taataatata gatgttagat
    33901 ttaatgaatt aaagaattat ttctttataa atagaggttc tctagtagtt atttctataa
    33961 taatagtttt ttatatagca agaaaatcaa cggttttaaa
//
LOCUS       DAMJVX010000021        33121 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMJVX010000021 DAMJVX010000000
VERSION     DAMJVX010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33121
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.023"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            79..960
                     /locus_tag="PC407_001007"
     CDS             79..960
                     /locus_tag="PC407_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HDF2496466.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKKGVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(1273..2052)
                     /gene="hisJ"
                     /locus_tag="PC407_001008"
     CDS             complement(1273..2052)
                     /gene="hisJ"
                     /locus_tag="PC407_001008"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HDF2496467.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEDLIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(2592..2942)
                     /locus_tag="PC407_001009"
     CDS             complement(2592..2942)
                     /locus_tag="PC407_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HDF2496468.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(2958..3374)
                     /locus_tag="PC407_001010"
     CDS             complement(2958..3374)
                     /locus_tag="PC407_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HDF2496469.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(3393..3584)
                     /locus_tag="PC407_001011"
     CDS             complement(3393..3584)
                     /locus_tag="PC407_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HDF2496470.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(3735..4550)
                     /locus_tag="PC407_001012"
     CDS             complement(3735..4550)
                     /locus_tag="PC407_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDF2496471.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(4597..5535)
                     /locus_tag="PC407_001013"
     CDS             complement(4597..5535)
                     /locus_tag="PC407_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2496472.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDASNKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYIYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(5532..6404)
                     /locus_tag="PC407_001014"
     CDS             complement(5532..6404)
                     /locus_tag="PC407_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2496473.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEK
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(6429..8357)
                     /locus_tag="PC407_001015"
     CDS             complement(6429..8357)
                     /locus_tag="PC407_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HDF2496474.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNSL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(8469..8846)
                     /gene="gcvH"
                     /locus_tag="PC407_001016"
     CDS             complement(8469..8846)
                     /gene="gcvH"
                     /locus_tag="PC407_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDF2496475.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(8933..11059)
                     /gene="cdhC"
                     /locus_tag="PC407_001017"
     CDS             complement(8933..11059)
                     /gene="cdhC"
                     /locus_tag="PC407_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HDF2496476.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(11116..11922)
                     /locus_tag="PC407_001018"
     CDS             complement(11116..11922)
                     /locus_tag="PC407_001018"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2496477.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(11972..13339)
                     /locus_tag="PC407_001019"
     CDS             complement(11972..13339)
                     /locus_tag="PC407_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HDF2496478.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(13370..14314)
                     /locus_tag="PC407_001020"
     CDS             complement(13370..14314)
                     /locus_tag="PC407_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HDF2496479.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(14334..15104)
                     /locus_tag="PC407_001021"
     CDS             complement(14334..15104)
                     /locus_tag="PC407_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2496480.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(15160..16545)
                     /gene="lpdA"
                     /locus_tag="PC407_001022"
     CDS             complement(15160..16545)
                     /gene="lpdA"
                     /locus_tag="PC407_001022"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2496481.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVIEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(16589..17470)
                     /locus_tag="PC407_001023"
     CDS             complement(16589..17470)
                     /locus_tag="PC407_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDF2496482.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMQKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(17506..18180)
                     /locus_tag="PC407_001024"
     CDS             complement(17506..18180)
                     /locus_tag="PC407_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HDF2496483.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(18217..19089)
                     /locus_tag="PC407_001025"
     CDS             complement(18217..19089)
                     /locus_tag="PC407_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HDF2496484.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(19181..19813)
                     /locus_tag="PC407_001026"
     CDS             complement(19181..19813)
                     /locus_tag="PC407_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HDF2496485.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKIEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(19918..21594)
                     /locus_tag="PC407_001027"
     CDS             complement(19918..21594)
                     /locus_tag="PC407_001027"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDF2496486.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(21843..22622)
                     /locus_tag="PC407_001028"
     CDS             complement(21843..22622)
                     /locus_tag="PC407_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2496487.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(22932..24851)
                     /gene="cooS"
                     /locus_tag="PC407_001029"
     CDS             complement(22932..24851)
                     /gene="cooS"
                     /locus_tag="PC407_001029"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2496488.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(25458..26369)
                     /locus_tag="PC407_001030"
     CDS             complement(25458..26369)
                     /locus_tag="PC407_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2496489.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(26485..26607)
                     /locus_tag="PC407_001031"
                     /pseudo
     CDS             complement(26485..26607)
                     /locus_tag="PC407_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            complement(26690..27832)
                     /locus_tag="PC407_001032"
     CDS             complement(26690..27832)
                     /locus_tag="PC407_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2496490.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYKVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(27850..29604)
                     /locus_tag="PC407_001033"
     CDS             complement(27850..29604)
                     /locus_tag="PC407_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HDF2496491.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMKINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            29740..29946
                     /locus_tag="PC407_001034"
     CDS             29740..29946
                     /locus_tag="PC407_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496492.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(29951..30874)
                     /locus_tag="PC407_001035"
     CDS             complement(29951..30874)
                     /locus_tag="PC407_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496493.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(31128..32828)
                     /locus_tag="PC407_001036"
     CDS             complement(31128..32828)
                     /locus_tag="PC407_001036"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDF2496494.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
BASE COUNT        10037 a         6548 c         3670 g        12866 t
ORIGIN      
        1 aacatattga aggtaaattt aaaggtacta tatactataa atatgtcgac acaataaatt
       61 taaaaaggag aactaaatat gaaaaaaaga ttaataataa tgatattgtc tgttgtctta
      121 gtactatcaa gtatacttac gatatttgca tattcaaata ttaaatataa taataataaa
      181 ttgatatatt ctaatatgat agataaaaaa actcaaaata gtgtaaagga aatcctaaaa
      241 gaaaataaaa ttaatgaaaa agacatagat acatttatta aagcagtcaa caattataat
      301 aaattgcaag ttaaaatact tcaaaataat ataaatatat ctaaatctgg atattcttca
      361 atcaatgcaa aacaagttcc atataaccta gaaaaattgc aagataattg ggtgaaaaaa
      421 ttccctgatt atatggatgt aaattgtaga ataactgctt ttagactttt taaagatttt
      481 ataaattcaa ataagaaatt tactggagac tctattgatt tgaatgtcga cttagacact
      541 ataatgaata ataaagatgc taaatttagc accaaagatg tagaaaaatt tatcaacttc
      601 ttttctgcaa taccagctaa agatacagat gatacaatca aaattgcaga acaaataaaa
      661 aatgaatgga aaaaaagaaa aatatctttt aaaaataata aaaatatatc tattataaat
      721 ggatttcttc gttatccaga aactaaaaac gtatttatag gtcatacagg catatgtatt
      781 aaaactaaaa atggcattct atttttagaa aaatatggtg ttacatcacc ttatcaagtt
      841 actaaattta aaaataaaaa aggtgtaaaa aattatatgt ttaatagatt gaagatgtca
      901 gaaggtgaaa ttgaattgcc tgaccctata ataatggaaa acgataaact tatgaaataa
      961 taactttaaa cgcaaaatag tatttataat tactaagtca atataataga gaaggtctat
     1021 agatttgtgt aaatacaatc ttatctacaa tcttatataa gaatgtttat ataaataaga
     1081 tagattaatt ctaagtccta ttattctatt gaattttatt attattttac ttagattcta
     1141 ttgttatttt acttagaact tattgttatt ttatttaagt tctattacta ttttcaatca
     1201 tataaattta caaaagactg tacataaatt tattttatac aaacttatct acagtctttt
     1261 gtgtgaatat atctaaataa tatcgtattt atttatttcc tctgcacagt gagaatcaga
     1321 acctaaaact aatggaatat cgtatttttt taccaactct aataagtatc catctatata
     1381 aggctctttg caatactctt ttctatttcc agaaacatta taatcaagtt cataaccttt
     1441 ttctttaatt aactttatta agtcttcaag aattgtattt cctttataat cgtatggaaa
     1501 tacctgatta aactttctaa ctaaatttaa atgacctatt ctttttggtt tatgtatacc
     1561 taaatctgaa tttacagctt ttatcaatgt ttcataatac ttattataaa cagcttcaat
     1621 acttcctaat ttgtcgatta aattttgaaa ctcctctaca ctgtaatcta cacaataata
     1681 atcatcatca attttaatta tatgtactga taaaagagag tcttctaatt cctttccata
     1741 tttatttaaa ttctccttta tcccttcttc gtatccttct ataaaatcca cttccgaccc
     1801 aacatttatc ttgattttac cctcatactt ttctttcaac ttcttgacat cattaaaata
     1861 ctttggtaaa tcttcaatac tcatacaact atcattttca ggagcagggt ctctaaatcc
     1921 atttgcatac ggaaaatgtt ctgtaaatgt catttctgtt attccaacat ccattgctct
     1981 ttgtacatat ttttcaaaat catccttact tccatgagga caataaggtg aatgtatatg
     2041 accatctttc ataaataatt cactcctttt ttaaagaaaa aatctaatat aaatgcataa
     2101 attttttaac ttcaaaataa tatttttaat catgctaatt tttattatat ctaatattag
     2161 agtaatttta aactgccttt taagtatctt atctttagta ctattttcaa cttaacatta
     2221 atattatttt ttaacttaac attaatacta tctttaaata gtaatactca atatataatt
     2281 aatcaaataa ctcaaattgt ttgatgaagc ttttgcagta tttaaagaat atataattat
     2341 ttaacattaa ataatccaat cttgtaataa aatttactca ttaaagtata ttacagaaaa
     2401 tatctcaaaa taaacttttt agttcatgat atactctttt ttgcatgaaa ttagatatat
     2461 ttttatcatt tcaacaatga aagggaggct tacatccatc ttgagataac cccttagata
     2521 aaatttttaa taattaaaca tcataaaata taaaaatcgc ttatatcaaa cgtataagcg
     2581 attaattata tttacatatg ttttttagcc tctaacaaca ttttattagc ttcttccata
     2641 tactctatag ccttttcaat actctttgct gtttcttctt ttccaatagc tctagccttt
     2701 tcaacccact ctctaaatcc ttcttcatga gtttcattgt gatttatcca atgtactaaa
     2761 agtattttta aagttttttc atccttagac tctgatgatt cctcatgact atgttcatgg
     2821 tcgtggtcgt gagcatggtc atgtggatgt tcatgacata tatctccatg agtatgttcg
     2881 tggcagtggt catggtcatg atgtccatga tgatatctta caatctcttt atcaaaaatc
     2941 attagttatt ccccctgtta ttttatatta aactcttttt ttataaggtt tacagaatct
     3001 tttacaagta gtgctaatgg tttaccttct aatccaacta actccacatt ttctggcata
     3061 ataggtaaaa gtatcttaag tgcctcactg tctgcaatag cttcacttat attacaagta
     3121 acttctccca tcatagaatt aggtattatt acagaaatag gtgcaactat tatatttgct
     3181 tttttagctg atactactat agcattttcc cctgtagctc ctttattagc atgagctttc
     3241 atcatagaac tagttgctat agaattagtc cctagtgcat aaatttctac atatgtaggt
     3301 aactcttctc tcaaagaaga aactatctgg gctccaatac ctccacccat tccatctatc
     3361 actgctatta tcatatgaaa ttcccctatt aattaatttt cttgtattat tatcttatgg
     3421 tccatcaatc ttatttcttt aattatccca tctattatct tttgctctcc taataaatcc
     3481 gttaaataaa tctttccatc acaagggtct atattaacca cattttccat aaccttttct
     3541 aactcattat tacttttaca aatgaaagct gatgattcac acatataaat ctcctcctaa
     3601 tatgattttt attaaaaatc aaaaaaagat tcccttaggt atttcaatac tccaaagaaa
     3661 ccttcatgat ttcagttttt gtatattatt ataccttaag tatatcactt taatttatgt
     3721 aaagtcaatt aaagttaacg ctttttatta tactcttcat tcttgtattt ttcatatagt
     3781 tctttaacta attgcttctc ataatcagtc aaatcgccct ccacaaactc aacattaaat
     3841 tgttcagcta atccctttac tatgtttttc tgtagaatat ctatgtctat ttttctgcca
     3901 atttcatttt ctattccact tgctttttta gctgtaaatt tcatagctct ttctttcaat
     3961 tctggtgtct tagtttttat taatttaaac aatttttcta catcaaaatc cagtattatt
     4021 gaaccatgtt gtaataaaac accatctttt ctgctttgag cacttccaat taccttctta
     4081 ttattaatag taacttcata tgatgcatgt gcattaaaac atgcagctga taaattttcc
     4141 ctacttattc tctcgcctct atttaaatta tctgtttcta taccacttag attcaatcct
     4201 ttaactagac cttcacttat atatctataa gataggttga tgctcttatc gattaaagga
     4261 ttatcttctc ctataattat actataagtt agctcattat catgtaatac agctcttcca
     4321 cctgtaattc ttctagtata gtctatattc atacatctac acttatctaa atctatctca
     4381 tcttccaatt tctgaaaata tcctatactc aaacaagctg gttcccatgt ataaaatcta
     4441 agagttggct tattatgtcc tttcttatat gcagtaaata tagcttcgtc aattgccata
     4501 ttcattgctc cttcatatga tttattatgt ataactctcc actgattcat gattattacg
     4561 cccccatttt tagtataaat atatcctaga aatcctctat agatttattt ctcttattgc
     4621 taaatctatc tcactttgat ttaaaggtct tgaatagttg tatatagtac ttccgggtct
     4681 ttcattatag tatggtctat tacaatcctt acaacctctt atttcaaaag gatagccttt
     4741 gtttatgtct tttaaaatat cattgtcaag ctttatacta tttaagtatc catttttaaa
     4801 cttaaaatac tcttttggat aacctttatt tatcatataa gacattaact gtacccttct
     4861 ataactttct atgcttggtt gatttatctt ttccatttta gttcctctaa ctggagtaaa
     4921 tgcaaataaa ctaatcgtta catcattttc ctttaaataa atatataaat tgtatatatc
     4981 ttcatgactt tcacccatac caactattat atgtgtactt attttattgg gatatgattt
     5041 tgacatttca gttataaatt ttaacttttc atcatagtta tttcctttta ttttttcata
     5101 tagttcttta tttgatgcat ctattgctat tcctatttta tcaactccta ctgaaaaaaa
     5161 cttctttatc tgttcatcat tttctaattt tgcagatagt gaaattggca tgtctatctt
     5221 accacttata tagtttataa agtctaaaac agagttatgt gcttcttcac ttgccattga
     5281 ttgaatacat attctcttta tattttttcc tttataagct tttaaagcat ctaatatttc
     5341 ttcttttgaa aactctggcc aaattactct tgataattta tcttttctag tactactttc
     5401 tattgattga gaacaaaaac tgcatttatt tatacatttt tctccaatca ttatatatgc
     5461 tgtggtagga ggtatatcgc tttttttgtt taatatccca agctctattg ctgtcccaac
     5521 tgatagtcta atcataaaac gcaacactcc ttacactcat ttattgtcat attcatttct
     5581 atagccttat tctttgcaga ccttgatggt aatactattc tattgactcc acacattata
     5641 gataattggt ctagctcttt tctatacact cctcttggtc tcatacaacc tagattaatt
     5701 tcagtatctg gtaaattaat tctagcttca cataaaatat ctgctacccc ttctaactca
     5761 ggaggttcta cattttcata ctcagtccct tttgttggaa taaggactat aaatgtaagc
     5821 ttattaggag gatttttctg taggtattct ataatttcat attcaccctt tatttgtcct
     5881 ccttttaaac caatacatat atgtggtgct acttctgtat gttcttgtat tgtattataa
     5941 acttcaatat aatcttcttt actctttttc atcttataaa cttctcttat tgtctcatca
     6001 tcaaacacta aatcaaaaga aactatatct aaatacttac acaactctac aatgctatct
     6061 ttatccataa gacctaaatg agcattcaat ctatatcctt tctcttttaa gtttttaatg
     6121 gtattaatat gagtatttat agggacatct ccctcataac tacaaccgcc actcaataga
     6181 aagctagtta tattccttga ttgtactttt tcctcatatt cttctattgg caccatattg
     6241 tttaagtagt gaccgttgca atgggcacat ttcaaactac attgattgct tgtagtacta
     6301 agagctagtg tctggttagg ataagcaaac tctattttat ttccaaaatt ctttaattta
     6361 atatcaaatg ctattttcat tttttcagat agattcttat tcatagttta ttccttctta
     6421 agtacatttt actcttctag taatttcttt gctgttggga actgctctat attcgtatgt
     6481 ggtaagaaac atgcagaaat aaattcatcc atataacttg gatatacact aagttcaaca
     6541 taagttattt gattacctat ttcttcaagt ttattttttg catctttact tatgagagct
     6601 aagtaagaac caacaagtga actattccct atataagtaa atttttctct atcaatgtca
     6661 ggaagtaacc ctataattat tgagttttct atgtctaggt tgtttcctat accacctgct
     6721 atataaaccc tatctaatat actaaaatcc atacctaaac tatctacaag agtgtatgca
     6781 cctgaatata tagctccttt agctctgatg aaattatcta tatcaacttc attgactact
     6841 atatcatttt ctaaatcaaa ttcttcttta aatgctaata catactctcc tatctcatgc
     6901 tcattaaatc taactctctt attatctaaa tccctgtata ttttacctct tctatctatt
     6961 acacctttag taatcatttg acagattaaa tcaattatac cagaaccaca tataccaact
     7021 ggtgcacatt catcaataat ctttaatgtt ggctctaaag tttctttatc aataattact
     7081 ttctctattg ctcctgctga tgccctcatt ccacagctta tccctccacc ttcaaaagca
     7141 ggtcctgctg aacatgcaca gctcatcata taatctttat ttccaaatac aatctctcca
     7201 ttagttccta aatctataaa taaaacattt tcttcacttg accatatccc tgctgataat
     7261 acacctgctg ttatatctcc tcctacataa cttgcaacag atggagctaa gtacacatat
     7321 gcactgtcat ttacaaaaag accaacattt tcacccataa gttttggaga ttttaaaaat
     7381 ggtggtatgt aaggttcttg tcttaagaag tctgtataca cacccaagaa taaacttgtc
     7441 atagttgtat tcccggcagc cactaatgta actacatctt ccttatctat tccatttctt
     7501 tcatatattg tttttaatag tggatttata gtttcctcaa ctatagcttt atgaagtgtc
     7561 tctaatccat ttttcttagt tgaatatata attctattta taacatctgc tccatatttt
     7621 atctgtgcat ttccagaaga tgctttatct actacctctt ttgaatatag gtctactaaa
     7681 caaacaacta ctgaagttgt accaatatct attgcaactc cgtataagga attttcttta
     7741 ttaccttgtt ctatatttat gattgtaagt ttcttttgtt tttgaacata tgttattgta
     7801 accttaaaat cactctttct aaatacagtt ggcatttttc taagtatatc caatctaaaa
     7861 tctatttcat tatatcctag gctatttcta acatatcttt ccaatctatc tacatcactt
     7921 atattatcat ctaaatttgg ttcttccatt tctatgtatt tcttttttat atttgtttta
     7981 aattgaaggt tatgttcttc aattattttt ttagctcttt caaaaatttc cctatcctct
     8041 tttttattac taccttctat cttcatacca tgcatagaag atgatacttt agaaggaact
     8101 tcaatttcta tatctgatat tacctttgta caacaagcta gtatataacc ttgctcccac
     8161 tcatcatctg ttatatgtct tgttttttct gtatcaactt ttccattcaa taattttact
     8221 ttacacttcc cacaagacac gtttccatta caaggagcat ctatgaatat atctgcattt
     8281 cttgcaacct ctagtaatat atcaccctca ttacaataaa cctctttatt gtttggtgta
     8341 aaactcactt ttatcatgtt atccctccca atttgttcta tatatgataa aaggaaagta
     8401 tagctaaatt tgcgtgcact caaattttac tatactttcc ccaattataa taatatctta
     8461 atctttaatt attctaatcc agcctcgtat tctttatcac ttaataacgt atcaagttca
     8521 gccacatcag ctagttttac ttttaatatc cagttttcat atgggtcttc atttatacat
     8581 tctggctcat cttctaattt ttcattaact tccaatacct caccattaac aggagatatt
     8641 aaatcagaag ctactttaga agactcaact actccaaaat ctactccctt agttacagta
     8701 tcttctactt ctggtgtttc aacaaacaat atttctccta attgatcttg agcatagtct
     8761 gttataccta tatatacaac atctccatca attactttta cccactcatg atctttagag
     8821 tattttaatt ctggtaataa tttcatttcg atagaccccc aatattatat tattgattaa
     8881 attacttaaa tcatattaat attatatcat attattttaa attttccatc aattacatta
     8941 cactttccat agctaatgct ggatgacctt tttcttctaa gaaagctaat actccttcag
     9001 aatcgtcagc tatagtttca tcacaaacca tatcacagaa gttttctatt ccagtcattt
     9061 ctttaactgt agcatttaat ttatcagcta cataatcttt taattctttt ggcatccaaa
     9121 ctattctttc tggtccacct tctgcataag cgaatttttt agaagatatg aaatgtcttc
     9181 cgtgtcccat aaatcctgga gtttgaactc ctccacctgt catagaagca agttctccga
     9241 aagtcatacc aactggagta acacttgcaa attctctgtt aactacaaca aatccgtttg
     9301 cttctggcat tataccacag atacattcga agcatccaca agaagtcatt gggtcttcta
     9361 gtatagaata taatgtaaca gattcaactg caccttgaga tatttggttt acagtttcat
     9421 ttactgaatt ccatacacct gttctatcat ctaagcactc gcctttttct attggttgac
     9481 aaggacctgt tgggtctaat tctttagtag ccttagcatc taaccagcta actgctccac
     9541 aaagaccaag tctttcagga gtaacaacac aaacgtgagc tggtgcaaat gattgacata
     9601 agttacaaga atagaaagta tcaacacttt catcaactaa tgaagctaat ctttcatctc
     9661 tagcattgta cttagctatt gcttctcctt ttaattcaga cactttttca gcatctgtaa
     9721 ttatagttat ttcacactta tcaactactg attcaaattc atctaacatc ttagcatata
     9781 aaacttctcc tatatgctca agtctaaatc ctttatcaaa tgcatcttta gagattctta
     9841 cccaagccat atctctttgt ccaacgtgca ttacaccttc tatatagttc aagaagtagt
     9901 ggaaacgtct ttcaagaact ggctcgaaat cttcttgcat atttttacca gctattttaa
     9961 ctattacagc aagcggtaat cttaatacac catctgcatc aacttcatct atatttggtc
    10021 ctattatttc tatcttatgg tcttcaactt ctgttgcttc tttcttaaca acaagctccc
    10081 aagcttcagt tctgtttcca ccaaactcag caaacatatc gctctttcta attctctcac
    10141 cttcaaatgc tgctgcaaat gaaactggga taggtatttc agtaactttt atttttattc
    10201 ctctagcttc taatgaagta gctactatct tatcgtaatc tttttgagtt aataggttca
    10261 ttggaacttc aagaactgtt tggtcagtta taactgggaa tcctaaagct attgctccag
    10321 cacctgcaga tacaactaat tcacttaatg gtccaaatgc attaacaaat gcaggaactc
    10381 tattagctgt gtactctaat aatccgttta aatcaccagg agttaatcca ccgaatatta
    10441 atgctgctct tactgcaaca gatacaacgt gtataacaga agttacatca tatcctagag
    10501 gtataactct tagctcaaga cccattttta ctccagcttc tatagcttgg tctataactt
    10561 taccaactaa gaaagttagt aaacctttag attggtaatc ttttataact tttgctgctg
    10621 attctgcatc tggacactca ccaagaacaa ctgcaacacc aggtatatct cctgtaacta
    10681 atggtacacc aagtgatctg attatagggt cagttatatg acctgcacat ggctcactat
    10741 atggagcatc catagttgaa tattttaatg cttcaattac ttctgcagct aaagccgtag
    10801 ctaaaccagc attaaaagca tgttctagta aatgagttct atttattaaa gattttacta
    10861 cttctaaagc accttctaag tcccctaaag tgttcatctt ttgtcctgtt gcagcatata
    10921 tacaaggtaa tgaatatgct gtatcaggga aagcaacttt atgctctttt ccattctttt
    10981 ctatagcttc agctaacata gcttgagccg cacctaaagc ttgttctgac cctgtaaaga
    11041 ttatattata tagattcatt ctatttcccc ctttaatatt tacacctatt ttttattaac
    11101 tataccctaa aaaccctaga aagcaaatcc accttcattt aactctaaga aagagtctgc
    11161 atataaagat gccccgttga ttatgtcaca agattttaca gttttcatta attctgggtc
    11221 acatggattc attatagctg aactaaatcc attagccatt gccattgcta agaatgcact
    11281 atctaaaaca ggtctaacat gcttaggaca tccattagat acgttagata atcctcctgt
    11341 agttaaaagt cccatctcag tcatcatttt tatagcttct aaaacttcta cttgtttttc
    11401 ttgcatacct tttataacta agcataatgg gtcaaatagt atatcagttg gctccattcc
    11461 taacattaag cctttttcta atatttcttg gcagtaagcc atacgctcat cattatctct
    11521 tgggatacct tcttttgcac ataaacctat aaccattgct tcgtattcag ctgctatatc
    11581 tattaaatca aatcttgagc cagcatcagc agagtttatt attggttttg cattagttct
    11641 atcataaact ttaagtccag cttcgatagc ttttttatta gctgtatcta atgctatagg
    11701 aacattgttg aactcagatt gaagtaattt gattccccat gtcattattt cttcgccatc
    11761 tctttcggca ggtcctatat taaaatctat atagtgtgct cctgcttcta attgctcttt
    11821 tgctcttttt aagattggag ctgggtctct ttctgctaat gcttttctta ttgaaggtga
    11881 tatacaatgt attctctcac ctataatcat aaatttttcc atttataatg tcccccttta
    11941 aatttaatat taatagttag ttaatatttt attagtttgt ttggcatgct ttttcttggt
    12001 atccttttaa gaacttaggt aattccatag attcctctgg tcctataact acattccatc
    12061 ctggtaagtt atcttctata tcacctttaa gtactgcaac ttttcctgga atgataaggt
    12121 ctttacaatt agttacttct tctaccttac tttctttaat gaaagcagag attgagtttc
    12181 caccaaattt accagcagcc caagatgtaa gaactgaata tccacctgca tctggtatta
    12241 ctaaccatac tggaactttt gatctttcta tatcaccagc aactatgaag taagttaatg
    12301 caaagtcaac agtaactaat actggagaat tttcatctgg gttgttgatt ggataaatct
    12361 ttggttcaac tctcattggt ctttgtgggt cagtgtatat gttttgtctt agtgcaaata
    12421 atggtaatgc cttagcataa ctaatatcat ctattactat tatagaaccg tattttatag
    12481 tgaatattga tgataaagca acttccatca tagggtttga attagataat ctatttgcaa
    12541 atactattga aggatatcca aatgttctat cttgctcttt taaagctgtt cttctaactt
    12601 gtatagcatt agtgtaagta tcttttatgt tttctcctgt tacatctaat actaattctt
    12661 tatatccagc ggcttgaatt aattcaactg tttcatataa ttcttctaag ctaccagctt
    12721 ttacacctaa tggtaaactt gctcctttaa ctacttctat catatctttg tagttctctt
    12781 tagttgctcc atataccatt ggtctagcat cttttaatac ttcaacagct tctttaacta
    12841 cttgtgcatc atcacaagct aaaatataag ctacttctaa tccactgcct tttattttat
    12901 ctattaattt taaatacgca tctttatctc cacaatactc taaaacagcc atttcaactt
    12961 tcatttgttc accaattctt acatagtcaa cttttttcat gttagctatt ttagcatcta
    13021 ctgcttcatc actcatacaa gtacagaatg aaactgcata tctatttcta cttactaatg
    13081 ttttttcatg tctaaataat actgtttctc cacctagttc atactcagat gcaccagcac
    13141 caacttttaa agctttcatt aaaggtgcag tagcctctga taattttgct atagcgtcat
    13201 cagacatatg tgggcattta cctacttcta cagctcctga agcaaccttc atagagaaag
    13261 ccatacaagt tgggaatcca caatccttac agtttttctt tggtgttaat ttaaatatat
    13321 ctaaagcttt taatgccatt attttttccc cctaacctta acaatttaat taagctaatg
    13381 cgttaaccaa ttcttttata gtttctattg attttggatg acgaagtata actgcgtttg
    13441 aaccacctac taaaacactt gctgcagttg aaacttccat tgctatagtt ctttcttctg
    13501 gacaacccca atctggctca tcttctattg gagctatagc ttctttaaca tgacctactt
    13561 caaaagctac tggtgttatg ataggcattt gtaatgtctt atcattttga ccaaatgctg
    13621 caagtctaat tctatccata gtagatgcaa cgtactcata tccataacca actgctgaac
    13681 atccaacatt cataactata ttttcacctt taacgcctag ttgagttaac aatacgttta
    13741 attgtttagc taggtttata tcaacagaag attcagctcc tactttatgt gcataagcca
    13801 taccagctga tgctcctact cctttgtagt tatcttctac tgctgacata aataagcagt
    13861 tatgtccatc caatgtttga gctaattttt caaataattt accatctttt tcatgatttc
    13921 ctgaaccagc tacaactaaa ggtaatttta ttgcttcaat tactgcctta gctacatcag
    13981 cacattcatc aactgactta tctaatccat ttgggtcaga accatcaaat tttaaacaaa
    14041 taaaatctgc ttgtgtatta gcttcaacat atttagccca ctctactgga caattagcta
    14101 catctttgta taattcctta tatgaatcag tccagctttc aggataaacg tctgatattt
    14161 gtatacctat ttttggagaa ttacctactt ctccatcaaa actataaaaa ggtaatacat
    14221 tttccccacc taatttaatt gctttttccc ctattcctac ttcaacttct gatatttttc
    14281 cagaatattt ttgagtagac attttaaatg ccatattttt ttccccctta agcctatttt
    14341 aattctaatt tagcaaaaat ttctttcata gccactttag attttgaatc ttcaggtaaa
    14401 ttaactaaag gaatacctgt agaatcatat tcatatatca attcatccat aggtacaaca
    14461 ccgattagct ctaggttatg cttttcaatt tcttctttaa caccatcatt cataacccca
    14521 tttggaactt tatttacaat caaaagtatt cttccaacac ttaactttag ttcctcagct
    14581 aaatctctta ttctagcaac agcttgtatg ctacgtcttg agcaatcact aaccaataaa
    14641 agtgtatcaa catgtctagt gacttttcta cttagatgtt ccataccagc ttcgttgtcc
    14701 atgactaagt atttgtaatg accagatatt ttgtttactt gttctctaag tatcccattt
    14761 acaaaacagt aacatccttc accttctgac ctacccatta ctaataagtc atatccttcg
    14821 ccttcttcga ttatagaatt taatctaaat tgaagatatt gtgcctttgt cataccacca
    14881 gggaatgcat tgcctaactt ttctctttga tttacttctt ctcttatttc tcctattgtt
    14941 gcttcaactt cgatacctaa cacttcattt atgttagcat tggcatcagc atctacaact
    15001 aatactggtc cctttttgtc cttaaccaaa taatcaatta aaagccctgt gagacttgtt
    15061 ttaccagttc caccttttcc tgcaacagct atattgtatc ccattttaag acttccccct
    15121 taaatctagc cacagagcct tatgtactct gtctgtctat tataatttag gtacagaatg
    15181 aacacattct ccatgtacat catgaagtgc ttccattagc ccttcagata aacttggatg
    15241 tggatgtatt gcatccccta cttgttctac tgttaatcct agatgaacag caagtgacaa
    15301 ctctgtcaat aaatctgtag catgaggtcc tactactgct gcacctatta tcttatcagt
    15361 ttctttgtca gctataactt ttacaaatcc ttggaaatgt cctatagctt gtgcttttcc
    15421 aagacctcta aaatcaaatt gacctacatt gtattctaca ccttcagctt caagttgctt
    15481 ttcagtctta ccaacaccag caacttcagg ttcagtataa acacatcttg gaattgctct
    15541 ataatctact accttagtct tacctaaagc attttcaact gctacgatac cttctttaga
    15601 agcaacatgt gctaggaaag gagtatctat tatatcgcct attgcgtata taccttctac
    15661 attagtttca agatgttcat taactactac ttttcctctt tccatttcaa ttcctatatc
    15721 ttctactcca gaattgtcaa ggttaggtct tctaccaaca catactaatg catattgtgc
    15781 ttctataacc tttccattag aaagagttgc aaccgcctta ccatcaacta cttcacaagt
    15841 ttgtactcca atacctgtaa taaccttaat tttatctttc ttaaattgtc taagtagttg
    15901 tttagctaca tctttgtctt catttaatag tatttggtca accatctcaa ctatagttac
    15961 ttctgttcct aaggctctaa agaactgccc tatctcacat cctataactc caccacctac
    16021 tattaacata gactcaggta tctcttcaag tccaagaact tcatcactag ttattacaac
    16081 ttttccatca tatgggaaca ttcttggtac aactggaaca gaaccatttg ctagtattat
    16141 cttatctgct tttatagttt ctactgttcc atcatcttta gttacttcta tagtgttttt
    16201 atcaattaat tttccaaaac cattaactaa gtttactcct cttttttcaa ataagaactc
    16261 tataccactt attaattggt ttacaacttt attttttctt tccattatgg cagtgaagtt
    16321 tggcttaact gtaccatcta tttctatacc aaaatccttt gcttctttta cagtattcaa
    16381 tactcctgaa gaagcaagaa gtgctttagt tggtatacat cctgcattta agcaagttcc
    16441 tccaactctt cttttttcaa taaccgttac atctgcacca agcatagaag cttttatagc
    16501 tgctacatat cctcctggtc caccacctac tactactatc ttcatcctta attcccccaa
    16561 acctaatata atctacaatc ttgcaatact ataaacctgc ggcatccagt atagcaggta
    16621 tattctcttc tgccccaatt gccattatat gaacaccatc acataaatct tcttctttta
    16681 attgtttgat aagttcccct gccattttta ttccttcact tacccagttt tcttttccag
    16741 ctgctcttaa tctttcgatt tgttcatctg gaacgaatat tcctggtaca tttgcagtca
    16801 taaatttagc cattcctggt gattttaaag gtactattcc tgctaatacc ttgcaatcca
    16861 tatcccttgt taattctcta aactttctca ttgtatttat gtcataaagt gcttgtgttt
    16921 ggaaaaattt agcccctgct tttatcttct tttgcatttt taataattga acttctattg
    16981 gagaatattc tggagttact gatgctccca gataaaaatc tggagaacct tttaacttat
    17041 taccagccat gtccgtacct gccattaaag tctcagcagt ttgaagtatc cccacagaat
    17101 ctaaatcaaa cactccctta gcctgtggat ggtctcctac gctagtatgg tctcctgtta
    17161 gtgccaacaa attgtttatc ccaaatacac ctgctgacaa catttctcct tgtatagcta
    17221 ttctatttct atctctccct gttatttgaa taactggctc taatccagca tcttttaata
    17281 atttacatgt agcaagagaa gttgctctca taactgccga ttgaaagtct gttacatttg
    17341 ctgcatgaac tcttccaact aacggctttg cacattctat taaatgtgaa aggtcagtcc
    17401 cttttggagg agccatttca gtagtaactg caaattttcc actttctaat gtttctctta
    17461 ataagctcat ctctaatccc ccttttaacc ttctaaaaaa aacttttatg aatttgctgc
    17521 tgcttctttc tttttctttg cacttaaact tcttggatta ttttgcttag aatagtcttt
    17581 tggtggtctt atttctgcta agttatctaa ttgtcctata gcttctagtc tttcatatat
    17641 ctttatccaa gcacaatcat tctctgagtt tacttcacac ttaccatttt tagctccacc
    17701 acaagcccca ttcataagac ctttagcaca catagttact ggacatatcc cacctgtcca
    17761 accaagttca cagtctccac aagccttaca ggcttcttca tattctccaa ctctttgtac
    17821 ttcccctata aacaaagtat tgttggctgg ataaactggc ttatctttta aattcttaac
    17881 tattgtctgt gtaccatctc cacatgctaa agataaaact gcatcagctt ccttagtctc
    17941 ttcttttaga gcctttaaat cctttttaga ctttaaaagg ttacaagcag ggtcaagcat
    18001 tacatatcct aagatttttt tgccttcacc ttcaagagtt tccttcattt ttaaaacttc
    18061 ttcttcccca ccacttttac aagttgctgc acattgatta caaccaacta aaaccagctt
    18121 gtcaaaatcc tttaagtacc ctagcacttc ttctaatggt ttattttcag aaattatcat
    18181 atctacttcc cccctaaacc ttccacatat atatctctat ttattgtttt gtaatttaca
    18241 agccttaact acatgttttg caagtataga tgttgtaact gaaccaaccc ccgctggaac
    18301 tggagttatc attgatactt tgtctaaaac tgcatttgta tcaacatctc cacataaatt
    18361 tccttcttca tcaacattta taccaacgtc tataactacc gctccatctt taacaaaact
    18421 ttcatcaacc atcttagctc taccaatagc tgctattagc acgtctgcct ctgctgctac
    18481 acctgataga tttttagttt ttgaatgaca tattgtaaca gttgcatgtt cacttaataa
    18541 aagcattgat actggctttc caactaccat tgatcttcca agaactgtta ccttacttcc
    18601 ttttaaatca acattatagt gttttaatat ctcaactact gctgtaggtg tacatggtgg
    18661 aaatccactt ttatcccctt ccattacctt agcagagttt attggactaa aacaatctac
    18721 gtctttttcc ggtgcaataa catattttat aacttcttca tttaagtgtt ttggaagagg
    18781 tctaaaacat aatataccat taacattttt gtcatcatta actctcttta atacatcaat
    18841 atattcctct tgtgttatat cctcagctaa ctctaacact tctgttgtta tacctatgtt
    18901 ttgacatctc tttagtgctc ctctttcata agataaatca tcacttcttg ccccaactct
    18961 aactattgtt agtttaggat ttataccttc tttaactagt aaatcgactt cttttattaa
    19021 ttcttcacta attttatcag ctacaggttt tccttttata atttgtcctt tagttgacat
    19081 tccttccata cattcacaac tcccctacaa gcaaacttaa attaatttat tctatatacc
    19141 acatattaat aacatagtat ctactttcgt catttaaaaa ttattttcca agagcttttt
    19201 caactttggc ataaacttca tcacaaattt ttacaccatc ttcaactaac ttatctactt
    19261 ctatttttac cttattaaca tattctttat cttgtataga atttatattt ataactacat
    19321 ttaattgtcc accaagtata gcagctctta aacactgaac tcctacacca acatcactta
    19381 tagcaagtct tgaaccttta tcaactaaat cttcatgtaa cttaatagct tcatagcaaa
    19441 ctcttactat gtttaatggt acttcacaag caacttttaa tgccttttcc attgtttctg
    19501 cttttattct tttttcttct tctgtttctt taggaagccc gtaagctttt gataatggta
    19561 agaaacattc tgcatcatca tctatcattt ttaataaatc tttttctaac tttcctgcct
    19621 tacttaatat ctcttttacg ctttcttcat actcagcata cttcttcttc cctattgtaa
    19681 ggttacatac catactacct aaagccatac ctattgcacc tactagtgca gcagctcccc
    19741 caccacctgg tactgcttct ttggaagata gtacttctac aaaatcaaca caagttttat
    19801 ctgctatttt catgataatc ctccttgaaa ggatgtagga aaatatctca aattattcga
    19861 gatattttac gaactatttt aagatatctc ctactcttta tttataaatt aatttcctta
    19921 gaataatcca gctattactc cattttcatc aacatctatt ttttctgctg ctggaacttt
    19981 tggtaatcct ggcatcttca ttatttctcc agtaagtgca actataaatc cagctcctgc
    20041 tgaaattgtt gcttgtctta cagttattct aaatcctgtt ggtctaccta atttagtttg
    20101 gtcatcagtt aaagaatatt gagtcttagc catacatact ggaactttag taaatccaag
    20161 tttttctaag ttagctattt ccttatcagc ttcaggagta taatctacac catctgctcc
    20221 ataaatctta gtagctatag catttatctt gtcttttata gataaatcat cttcatagca
    20281 gaatctaaag ttattttctt cttcatccaa taatcttaaa acttctttag ctactgcaat
    20341 tccaccttct ccaccttttg cccatacttc agaaagagca acattaacac ctaattcctt
    20401 acacttgtct tcaacaagtt taagttcagc ttcagtatca agtgggaatc tattgattgc
    20461 aacaactgct ggtaatccat atacttgagt tatgttttca acatgtttta ataagtttgg
    20521 aagtcctttt tcaagagctt ctaggttttc gttatttaag tctgctttag caactccacc
    20581 attgtatttt aatgctctta cagtagcaac tattataaca gcatctggtt ttaagtttgc
    20641 cattctacat tttatatcta agaatttttc tgctccaagg tcagcaccga aaccagcctc
    20701 agttattaca tagtctgcaa agtgcatagc cattcttgta gctataacag agttacatcc
    20761 atgagctata tttgcgaatg gtccaccgtg tacaaatgat ggtgttcctt caagagtttg
    20821 aactaggttt ggttttaaag catcttttaa taatgcagcc atagcaccat tagcttttaa
    20881 ttgtctagct gttacaggtt caccttcata gctgtatcca actattatgt ttcctaatct
    20941 ttcttttaaa tcagatatat cacttgccaa acagaacgct gccattattt cagaagcaac
    21001 agttatatcg aatccatctt gtctaactgc tccatcacct tttttaccca taccatcaac
    21061 gatatttcta agttgtctat cgttcatgtc cacacatctt ctccaagtta ttttcttagg
    21121 gtctattcta agtgcatttc cttggtgaat atggttatca agcatagcag ctaaaagatt
    21181 gttagcagct cctatagcgt gaaaatctcc tgtgaagtgt aagtttatat cttccatagg
    21241 aacaacttgt gcatatcctc caccagctgc tccaccttta acaccaaata ctggtcctag
    21301 agatggttct cttaaagcaa ctaatacatt tttgtctagt ttagcaaatg catcagcaac
    21361 acctatagta gtagtagttt ttccttcacc tgctggagtt ggatttatag ctgtagttag
    21421 tattaactta gcttttttac cagtctctct ttttagtaga ttgtagtcaa cttttgcttt
    21481 gtatttacca taaagttcta catcatcttc acctagtcct aatttttttg caacctctct
    21541 aatatcttga ggcttagctt cttgagctat ttcaatgtca gatttaaatc ccataactga
    21601 atctcctccc acaacctaaa tagatttaaa atataatttt tgtaaaaatt gtattaatat
    21661 gtaaaaatta tattttataa gataattata catgtaaaat tacttaaaaa ctagctacta
    21721 agctattaaa atattcaaac ttaatagcta gttatttatc taattactta cttattacct
    21781 atctattgcc tatgtctaac ctttaaccaa cttaaaatcc caaatcaaaa tttctatcta
    21841 ttttatttat ccttaagtga cattaattta tcttttattg ctttaatctg ttctttcgtt
    21901 gtttcactag aatcatatgg agattgatta gctctatcag aatcaataac atcttggtta
    21961 tagtaaataa atcctaaaga ttctccatct tctaagtttt ggattataaa ttcttcatct
    22021 tccttatttc taattttatt tcctactaca aatactttat ttacacctat atcatgtcca
    22081 agtttcttaa cttttctata agtctgtaga cttctctctc ctggttctac aactactata
    22141 aaagcatcta caccttgggc tgttcctctt cctaagtgtt caattcccgc ttccatatcc
    22201 ataaccacta catctttatt ttgtagtatt aaatgtgagc agagtctctt caaaagaaca
    22261 tgctctggac atacacatcc agtaccacca gaatctacgg ttcccaatgt aagtaatctt
    22321 actccattgt attctttaca gaaattttca gggatatcgt ctactttagg attcatctta
    22381 aacatttttc caaatgaacc tacagaagca gctgttctat ctgaaactaa ttttttcatt
    22441 tctgatatag gtactattga ttcatatact tcttttggaa aacctagtgc taaagctaaa
    22501 ttagcatcag ggtcggcatc aacagcaaca actctgtatc cgtcttcagc aaacatccta
    22561 gagagcattg aagaaaaagt cgttttacct actccacctt tacctgttat tgctatcttc
    22621 ataatcccta ccacctttta ttactatttg ctttttagta gttaatttta cagttaaata
    22681 ttttttatac tctatatttt tatacactaa aaataaacta cagctagtcc ttacaggaac
    22741 caattaaatt taccctacta gcttttcaaa gcagcatgta tagttttatt attctttaat
    22801 attgtttata ttttatttat tcagtatatt cattatattt cgatgttctt tactttaata
    22861 tttgacttta ttcaacttta ctcaaccaga aacttcttat aaaaacgttt attctataac
    22921 ttcactattt tttaagcttc agccttttct gccatttttt cttcaactaa tgcttcaaag
    22981 tgaacacgtt ttttctctat atcttctatt attgtagctg ctaactcttt tggatttgta
    23041 tttacagtaa atgtagctcc aactttatct tttaactcac tagttaatat atccactgct
    23101 cttgaagaac cagctattgg aggcattatt cctaaataag tgtctatacc agaagctaca
    23161 acatagcatc ctatagcaac tgctttttct gacatccatt ctggagctat acctacaact
    23221 ggaaggtcac acatatccat atctaagtaa ttagctgctg ctccaactaa atctagtata
    23281 cgacttatat caacacaaga acccatatgt agtactggtg ggattcctac taatttacag
    23341 acagttgcaa gacctttacc tgcatatttt tcagccgcat cagattccat taaaccaaac
    23401 ttagctgctg ctgaagctcc acaacctgta gtaactacta ttatgtcgtt tttgattaat
    23461 tctttcatta ttgttacgtg agcctcattt gaaactcctt tagcattgtt acatccaact
    23521 acaccagctg ctcctcttaa tactccagct tttagacagt ctgttaatgg cttaactgtt
    23581 cctgctgggt ctatatgaga gtttacaact ctgtctaatt gatttactac tgcttctaca
    23641 ctatacccaa ctgttgcgcc tgattttaat tcaggtataa gtacttttga tttatctcta
    23701 tttttaaagt ttaaaatagc ttctttaact attgctttag catcatcaag tgcttgttct
    23761 tctctaaatt caatataagt tgaacctgtt atcttagctt ttggagaagt tgttacaaat
    23821 ttagtatggt aacaatctgc aactctagct aatgcaggga atatacattg aacgtcaact
    23881 attacagctt caactgctcc agttattatt gcaagttctt gttgatggaa gtctcctgct
    23941 atttttacac catgtctcat tgtaatctca ttacctgtac aacacatacc tgctaagttt
    24001 attccatcag caccaacttc tttagctaaa gctactaagt ctggctcttc tgatgctaaa
    24061 actatcattt ctgataatgc tggctcatgt ccatgtaata ttatgttaac tttattttct
    24121 tctaatactg ctaaattagc ctctgttctt cttggcactg gagttccaaa taagatatcg
    24181 cttagacgag tacctatcat agaaccaccc caaccatcag ccatagaagt tctaagactc
    24241 atgtgtatta aactatctat atcaccagta catcctatat gagtagagtg cattattgtt
    24301 gcaatttctc tatctatagc tcttggctct atagcgtatt ctttccatat tttttgtctt
    24361 ggttctggtg cattttttat aaatcttaat gtcccaaaag gttttccaaa ttccattaat
    24421 gcaatttctg ccacttcatg tgctacatca tagatatctc taccttctgt ctctacatcc
    24481 cattctttag ctaaagtaat taacttagct tcgtctctta ttttatagtt tccatttgga
    24541 tcagctaatg ctaatgtatg tgctatatct ctagcatggt ctgaatgggc agctgtccca
    24601 ccagcaacca ttcttgcaaa gtttctacct actatagtat gctcatctgc tccacataac
    24661 ccccttggag tcttagggct tattctacat ggtcccattc cacatattct acagcaaaca
    24721 ccttgaagac caaaaccaca ttgtgcctta aagccttctt ttctatcata cattgtttct
    24781 acgccttctt ctctagcttt agctatcatc ttctggctgt ttaaatcaat tgttaacatt
    24841 ttttcatcca tattcctatc cccttttttt gatttttaaa atcccccagg tgactgactt
    24901 catgtcataa aataataaaa taaatgcttt ctaagttgat tatatactaa ttttcagtgc
    24961 tttgctttag atttaaatat ttttttaaca aaattagata atacataaaa attttatttt
    25021 ctacacttaa tttaagttga ttttatcgct tgtttttggc tatttgttaa tatctaaacc
    25081 tacttttttg atacttcagt ttaattaaat tatgttttga tttttttaaa tcaaatttga
    25141 aatttttcta gtcattttta tattattgca gattgtttgt gtatttatta tctttcacta
    25201 ctgtttggac ttattacaga tttttactcc ctaaacttaa ttagtaaaaa cttcttagaa
    25261 tagcattttt tggactaatt tttttgaatt tttttatttt tcctacttta atattaatta
    25321 tagcttcaaa ttgaacattt gtatatattc atttgaagct ataattattt tttatataaa
    25381 acttttttaa tacagcatta atttagacat tttgactatt ttttctactt taattcattt
    25441 atttcaagcg atttatccta tttcatattt ttagctacaa tagccccata tgcaatagaa
    25501 tataatttag attcatgtga atcagctctt gacactaaaa ctataggagc ttttgcaccc
    25561 ataattatac ctgctgattt agagtctgcc atatatgtaa aggctttccc tattccattg
    25621 ccaacctcta ttgttggaac caatatgata tctacatctc cagatacttc acttttaaac
    25681 ccttttatct cactagcttc ttttgaaaca gctaaatcaa aagccagagg accttctaca
    25741 attacatttt caccaaattc tccttcttta caagcttttg ctaacatatc tgcatctaca
    25801 gttgcttgca tcttaggatt tactttctct ttagctgcca aacatgcaac ttttacagtt
    25861 tccattccta aagcttttgc agcttttata gaatttttta atatctttgc cttttgttca
    25921 taatcaggag ctatattcat acctccatct gtagttataa gtaatttatg gtatttttct
    25981 aactcatata ccataacatg acttaataaa ctatcagttc taagaccata gtctttattt
    26041 aacacttctt ttaatatgac agatgtatct aataatcctt tcattacaaa gtcggcttct
    26101 ttacttgata ctaatttaac tgctatttct gcacactctt tgatatcatc cttatccact
    26161 attttgatac cacttaaatc aaatcctatt tcttttgata tttctcttat tttgccttca
    26221 tttccaacca atataggagt aattatattt ctttctacag catcctttat tgccattaaa
    26281 acttctttat catgagctgc tgctacagag agtacaattt tctctcctcc cttaagttgt
    26341 tctaaaatat catctaattt ttttatcaaa agtaacccct cccttatata tatttaacta
    26401 aataatacca tatttgctat accttttctc aatttatttt ttatgttata aagaatttat
    26461 atgactaaaa gacagtttaa tattttattc tgggatttca tcaatagctc ttaaaagctt
    26521 tgcaggaaca ccacctacaa tagtattttt tgttacaact gctccagcag cgataattgc
    26581 attatctcca atagtaactc ctggaactct tcatacaaaa aagaggctta tctctatttt
    26641 gagatagccc cacttaaccg ataaaattaa atacaaccta acaatatcct tacttattta
    26701 ttccaagttc tcttagacaa gcaatagtaa tttcatcttg tggatttaat tcataagctc
    26761 tttctatata gtaagttgca tcatcaatat caccattatt taaataagcc attcctaaat
    26821 tgcaaagtat ttctgggtct tcctttatct tagccgcttg ttcaaaatac tctattgctt
    26881 tatctaaatt attcttataa gcctcacaaa gccccaattc cactatagta tccacctgat
    26941 taggctttat tatcaatatc ttctcaagat acatcttagc ctgctctatt tcgcccatac
    27001 ccttatatcc aagtgctatc ataaacagca agttccacca atccatatgt tcttcttcta
    27061 atggaagtaa cttctctaat ccttccttaa acttaccttg gaatacaagt gtatatcctt
    27121 cttcatattg aactttaaag tccatcttgc ctagattctc ttgtacctca gctactaaat
    27181 cagcatctaa ccctagcctt aatgcttctt cccaagtcag cttagtcttt aaatattgac
    27241 cttgattata ataatggtag cctaagtgat aataacctaa tgcaaaattc tcatcaacat
    27301 caattacctt ttcaagctta gccatggctt caagtaagaa tgcattcatt gccttatcat
    27361 ccatatcttt ttgatactgt ttagctatct cttggcaaac aatcgcatag ttatacattg
    27421 catctaaatc atctggatac attgttataa atgcttttat atatattaaa gaatctgtat
    27481 gttcaccaat ctcaaagtat tttctcgaca tatatcccat atacgatttc aaatccaaat
    27541 caatatcttt tgataaagca tttaaaaact tctcatactc tgcattgttt ttaaacttac
    27601 tatctattcc aattatatat atcattgcat ctgctataga cattgaattt aacttatctt
    27661 gagcagtctt ttcttttatc ccttttacta atacctcatt ttttataggt acatctaatc
    27721 cgccttttgg cactttatag ctttttaatt taaactctcc accatgtttt atagttataa
    27781 aagctaattc ttctgctttt tttagcaaat atttttctat cttaaaatcc attttatcct
    27841 cctaaatact taaatctcta ctttatgagt tttagatttc tcaaatattt ttttatcatc
    27901 atactcaaat aagactacat tttctacttc tcttatttct gtatttatat cattttttaa
    27961 atccacaaca ttaaatctaa atggctcaaa atgaacatat tttattatat gtttagctgg
    28021 tttatcaaca aaactaggta aatatgttga taagttatca ttatcttgta aaaatacatg
    28081 gcatctattc ttaaaatcat ccacatctag cttattaaaa aactgtggta tatttgatgt
    28141 tttacccagt gatatatagt catattttaa tatatcattg aatacatcat tatctatgtt
    28201 gattttttca ttatagaaat ccatcaatat tttatataac tgattctttc cttgtgccaa
    28261 atcaaagtag ccattttcat caaaatactt ggcaaaaacc tcaaagaatt taaaaggact
    28321 ttgtttataa aatctgccta taatatatct catagataac acaaagtttt tagaattgta
    28381 gtatctctcc agtatatctt ctatatcctt tagcttcaaa gtttcttcat agcttatata
    28441 attattgtat aaaaactcat atggagcata atccttatat ttaaaaccat gctcatctgc
    28501 aacttttctc atacctgtcc ctttaatcat ctttaaaaag cctaattgca gatgctctat
    28561 acctagatta aatacatcat taaaagaatt ttcaaagctc ttataatctt cataaggcag
    28621 tcctgcaatt aagtctaagt gctgatgtat atttctataa gatgctacag tttgtacaac
    28681 atgagacaac tttttaaaat catcccttct acctactgca tcaagtgttt tttcattcgt
    28741 tgactgaacc cctatttcaa attgaaaaag accttcttta caatcagcca aaaactcaag
    28801 catcttatca tctatcaaat acgctgtaac ttcaaaatga taagttgtat atccattatc
    28861 atttttcata aggaaatcca tgatttccat agccactttt ctatttgcat tgaatgtcct
    28921 atctataaac tttatctgtg atactcttgc atctattaaa gcttttaaat cactctttat
    28981 cctgtctaaa ctaaaatacc ttagacctgg aatagtagat gataaacagt actggcagtt
    29041 aaaaggacaa cctcttgatg attcataata cactattcta ttttcatatt cttttggatt
    29101 caagttttca taaggactag gaatctcatc caaatcttga agtaattcca taggcttatt
    29161 cactattatc tcattgcctt gtctatatac aagtccattt acatcattta ttttcatctt
    29221 tccttgcaag tgaagtacta agtctctaaa aattaattct ccctctccat aaagtatata
    29281 atccacaaaa tcatactttt tcattgcctc ataactatca taactcactt ccggtccgcc
    29341 caaagctatt ttagtattag gtctaatttt ttttagatta tcacaaagtt taactatatc
    29401 tcccacattc cagatatatg ttgagaataa tattatatca tattcatttt tatatatatc
    29461 ctttaaaata tagtccaaat cgttgtttat cgtgtattct ttcatattaa cttctatcaa
    29521 atctcttaca aattctttta gatatcttat agccaaattt gtatgaataa acttggaatt
    29581 taatgttgtt aataaaattt tcaaatattt ctcacctctt tttaagatta tactgtcttt
    29641 ttgtacccct tataggttaa aatagtatat gtaaagtcta aataatatct atataattaa
    29701 tttattaatt tcaagtaatg tgaggaaagg aagtttttta tggaattaat tgaaaaagta
    29761 tctatcaact ctttatcaaa aagagattta ctacttatta taaagtctct tgaatacact
    29821 aatgaaaata ctcaaattag tgatttcatt gaccttagga ataatatagt aaaggaatta
    29881 tgttttttaa ctgacactaa ggaaaaagat tttttagatt atctagaaaa aaatagcaca
    29941 ctctaataac ctaaaccttt ttaaacttta atatataaat acctgattta gaatttattt
    30001 ttatgcttaa tatattatca ttaaaggttt tcgacataac tgttatttga ttaaatatag
    30061 ggtctattat agtattacct tcttcaagtc ttataagact tcttgggtct atgcttttat
    30121 tatatgcctt aactccaatt ttatattttc tcaatttaac aacatattcg gacatagaaa
    30181 aaataaaatc tatattatct tcatttaatt tactatcttc acccaaaatg gataatatag
    30241 atttttttat gctattaatt ccttcatttt ctacagttct atcttccata tatacatcat
    30301 atagaacatt taaaacattt aataaaatac tttcttcatc ataatcttca gatttcaaat
    30361 caatgtactt atcaattaat tgtattgctt taatccttgc catctgaaac tttacaacat
    30421 ctgatttgtc taattctata gaaaatccta ttatcttgtc tttaatactc tgtagtaatt
    30481 cgacatactc tattttacta ttatttataa gattgtgcat aatggaatta tacactacag
    30541 aacctaataa ataatcaaat aaaagttctt cttttttaaa ataaataaca tactttttta
    30601 ttagtttaat aacctcatct tcgctttttt gaatattagt atttgataaa attagtggta
    30661 gtaaaatatt taaaaaattg atatttccta ttttttctcc ataaaatcta tagtaaataa
    30721 gttcatctct cagtctattt tcgtctatat acacacatct ttgcgtttta tcatctatta
    30781 aggattttgt atttattata gttaaatctt taagagccct acttgcacta ctaaaagaag
    30841 atgtgtagac tttttttagt aattcgtcat tcataaatat atcctttctg aaaaattgaa
    30901 tgtaaataaa ttgttattaa aaaaaaagag acagcccgtt taagaagctg tcatatatag
    30961 ggggatagta gtgtacaaaa atcattgtaa tctactatct atatattatt atacgatact
    31021 acttttttat atgtaaagca tcagttttcg acatccctat attttacagt tatacaaatt
    31081 gcgtactaag ttcaatttta ttctttttat ttcaacattt tatcatgcta cattctttcc
    31141 ggagcgtgca ttcccaacaa tttcagagaa ttttctaaag tttgttttgt tgctaaaact
    31201 aatgcaagtc ttgcttttct tatttccaaa ttttctacta aaattggatt gtcatgataa
    31261 aatttattaa acgcttgagc taaatctaac ataaatctag tcactatatg tggctcattt
    31321 ttctttagag caagtaaaat attcttattg aatgattcta taactctaag aacttctgca
    31381 ctatcttcat cagctaatag actataatct atatcagaag tcacttctac ttctgctttt
    31441 cttaaaactg cacagcatct agcatgtgta tactgaacat atggtccagt ctcaccatca
    31501 aaactaagcg ttctttccca agagaaggta tagtctttaa ttctactgtt agataactct
    31561 tggaatacta ctgccccaac acctacttgc ttagatattt catccacatt tttagcattt
    31621 ggatttttag ctagtataat ttccttagtt ttatctatag cttgatttaa agcatcttct
    31681 aaaaatacta ctcttccttt tctagtcgac atagtacctt cttcaagtgc aaccatacca
    31741 aatccaacat gaattaaatc ttttgcccaa tcatatccca taagctcaat taccttaaac
    31801 cattgttgga aatgtaagtt ttgttgagaa ccaactacat atatacactt gtcaaaatca
    31861 taagtctctt ttctatatat tgcagcagct aaatctcttg tcatatataa cgttgaacca
    31921 tcattcttag taataagtgc tggtggcatt ttatactctt ctaaatcaac aattcttgct
    31981 cctttagact cttttaaaag atttttctct tctaatagtt cgataactct gttcatttta
    32041 tcagaataaa aactctctcc tgctaaagaa tcaaattcta tatctaaaag tttatacact
    32101 ctgttaaact cttttaaact ctcatttcta aaccattgcc ataattcttg tgcttcttcg
    32161 tcaccatttt ctaatttatt aaaccatgct cttgcttcat cttccatttc tgggtgttgt
    32221 tcagcttcat catggaatct tacgtataat tttagtaatt ctggaattgg attagactct
    32281 actacttctt tttctcccca ctttttaaag gccactatta acttaccaaa ctgagttcca
    32341 tagtcaccta aatgatttat tcttatagtc ttgtatcctt gcgaatcata tatcttatat
    32401 aaagcattac ctataactgt agttcttatg tgccctatat ggaaaggttt tgctatattt
    32461 ggagaagaat attcaactat tacagtttta tcttttccaa aatcactatg tccgtaattt
    32521 tctttttcat ctaaaacttt tttaattaca gtttctgcta attgagattt atttacaaag
    32581 aaatttacat atccgcctaa ttgtattact tttgttatct ctccacttgg ttctatactt
    32641 tccgccaatt cacttgctat catgtttgga gcttttctaa acactttggc taatttaaag
    32701 catggaaaag catagtcccc catatcctta tttggtggaa cttctataag tgcctctatc
    32761 tcttctttcg ataaatcttc tattttttct ttaaggcaat ttgatattgc caccttaaaa
    32821 tcttgcattc tgttgctcct ctctacattg ttcctggttt atattgtgct tatctcttta
    32881 tgttaaatca aatttcgtgt tttttcaagt atttcattac agtgtaaaac actttaataa
    32941 ttatgttttc tgtatatatt aaaaatacta taattgcagt ttttaatcac actattattg
    33001 attttttata agactttttt ctattttatt gtttcttgta ctatccagtt gactttatat
    33061 acttttttta atcttttatc aattttcata catcaatatt aattttatat acatttattt
    33121 t
//
LOCUS       DAMJVX010000022        33096 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMJVX010000022 DAMJVX010000000
VERSION     DAMJVX010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.024"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            50..859
                     /locus_tag="PC407_001037"
     CDS             50..859
                     /locus_tag="PC407_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2496495.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
     gene            1004..1243
                     /locus_tag="PC407_001038"
     CDS             1004..1243
                     /locus_tag="PC407_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496496.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(1431..1607)
                     /locus_tag="PC407_001039"
     CDS             complement(1431..1607)
                     /locus_tag="PC407_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496497.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1661..1757)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2112..2786)
                     /locus_tag="PC407_001040"
     CDS             complement(2112..2786)
                     /locus_tag="PC407_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2496498.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     gene            complement(3006..3560)
                     /locus_tag="PC407_001041"
     CDS             complement(3006..3560)
                     /locus_tag="PC407_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2496499.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            complement(3638..4003)
                     /locus_tag="PC407_001042"
     CDS             complement(3638..4003)
                     /locus_tag="PC407_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2496500.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            4468..5253
                     /locus_tag="PC407_001043"
     CDS             4468..5253
                     /locus_tag="PC407_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2496501.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFDIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKEHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(5624..6802)
                     /locus_tag="PC407_001044"
     CDS             complement(5624..6802)
                     /locus_tag="PC407_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDF2496502.1"
                     /translation="MKTNKISFNKGLYYLNTNANFNFQLNRIIMWDGGRLEDIQKVSS
                     KIVDSESWKTELMYLAQEAEQDGRTENAIAYYRMSEFFMYDGDPDKISVYKKATTLFY
                     NYYSEYFSNGTVQIFHVPYENITLPVMYAKAQGEVKDVILLHGGNDSYFEEFFFPMLY
                     LASQGFDIYLFEGPGQGGVMRLQGKHFTYQWERPVKTILDYFHLENITIVGASLGGFL
                     APRAAAFEKRIERVVAWSVFPNFLDIIVGSQPIKMQNLLRLLLKLKTKWLVNFIIKQK
                     MKSGDSMVVWGLKHGMYAYEASTPYEYFQKIKYYQIDSIAKNINQDILILGATQDHFI
                     DYRTVGKEIDLLNNVRSLTFRLMTEAEQASNHCNCGNSKLVLDTISNWIMMMKIHDRV
                     "
     gene            complement(6905..7669)
                     /locus_tag="PC407_001045"
     CDS             complement(6905..7669)
                     /locus_tag="PC407_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2496503.1"
                     /translation="MTTICVMIDHIFLDTEYRQPTIHKHLAKHIIISQSGNLSVTFDK
                     GESLQCKGIIIDSNVSHTVSSKDNKMLVFLIDDTSILAQNISQHQLLNHSFFVIDNLI
                     VERIQNIYTACQVKGLSQSYIDFSKQAFRLLNLNYHISSITDSRIQWALNHILKSTHI
                     HNEIFFEICAEVKLSKSRFSHLFKEQMGVSLNSYLALSKLRKAYELLLKSGDITSAAM
                     DAGFSSPSHFSASSKKYLGITASELIGKCKIYFIRQ"
     gene            complement(7858..8682)
                     /gene="pdaA"
                     /locus_tag="PC407_001046"
     CDS             complement(7858..8682)
                     /gene="pdaA"
                     /locus_tag="PC407_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF2496504.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            8903..12022
                     /locus_tag="PC407_001047"
     CDS             8903..12022
                     /locus_tag="PC407_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2496505.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            12040..12639
                     /locus_tag="PC407_001048"
     CDS             12040..12639
                     /locus_tag="PC407_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HDF2496506.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(12896..15025)
                     /locus_tag="PC407_001049"
     CDS             complement(12896..15025)
                     /locus_tag="PC407_001049"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDF2496507.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            15291..15539
                     /locus_tag="PC407_001050"
     CDS             15291..15539
                     /locus_tag="PC407_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496508.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSTNKKQKSHSRDIKRKIS"
     gene            15705..17096
                     /locus_tag="PC407_001051"
     CDS             15705..17096
                     /locus_tag="PC407_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDF2496509.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKEDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            17211..18680
                     /locus_tag="PC407_001052"
     CDS             17211..18680
                     /locus_tag="PC407_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF2496510.1"
                     /translation="MSNVNKKLVIAIGAVALVAIIFIGIMCMQFKGEKIAKNTYVNGV
                     DIGKLTKSQAKQELAKKYKLENVEFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SSFFGNLSKTISTNFGKKSNLVVVINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLTNIDTNLGTY
                     STIFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            18745..19338
                     /locus_tag="PC407_001053"
     CDS             18745..19338
                     /locus_tag="PC407_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HDF2496511.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            19520..20866
                     /locus_tag="PC407_001054"
     CDS             19520..20866
                     /locus_tag="PC407_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2496512.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIRKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            21048..21419
                     /locus_tag="PC407_001055"
     CDS             21048..21419
                     /locus_tag="PC407_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDF2496513.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            21412..22101
                     /locus_tag="PC407_001056"
     CDS             21412..22101
                     /locus_tag="PC407_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDF2496514.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            22621..23487
                     /locus_tag="PC407_001057"
     CDS             22621..23487
                     /locus_tag="PC407_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2496515.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            23729..24538
                     /locus_tag="PC407_001058"
     CDS             23729..24538
                     /locus_tag="PC407_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496516.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKKGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGQGSYTFFVDEGT
                     AMEYIGEFKEDKKNGKGNFVFSNGNEYIGHFEEDMMNGIGNMLYNSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            24681..25745
                     /locus_tag="PC407_001059"
     CDS             24681..25745
                     /locus_tag="PC407_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2496517.1"
                     /translation="MKRLIALVLVILIVLFYSPELIEEGYDLKEKFFQSEIWNEFLDK
                     ININNNSKKDKKEQNQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     KLYPDDREELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HINKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            25773..26492
                     /locus_tag="PC407_001060"
     CDS             25773..26492
                     /locus_tag="PC407_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2496518.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVKNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            26516..27100
                     /locus_tag="PC407_001061"
     CDS             26516..27100
                     /locus_tag="PC407_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDF2496519.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGYVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIIDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLENFITEARERVHM"
     gene            27102..28451
                     /gene="pabB"
                     /locus_tag="PC407_001062"
     CDS             27102..28451
                     /gene="pabB"
                     /locus_tag="PC407_001062"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDF2496520.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDTHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            28441..29184
                     /locus_tag="PC407_001063"
     CDS             28441..29184
                     /locus_tag="PC407_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDF2496521.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            29218..29919
                     /locus_tag="PC407_001064"
     CDS             29218..29919
                     /locus_tag="PC407_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496522.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISNIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELASKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            29945..30517
                     /gene="folE"
                     /locus_tag="PC407_001065"
     CDS             29945..30517
                     /gene="folE"
                     /locus_tag="PC407_001065"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDF2496523.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            30583..31374
                     /gene="folP"
                     /locus_tag="PC407_001066"
     CDS             30583..31374
                     /gene="folP"
                     /locus_tag="PC407_001066"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2496524.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELEIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            31385..31750
                     /gene="folB"
                     /locus_tag="PC407_001067"
     CDS             31385..31750
                     /gene="folB"
                     /locus_tag="PC407_001067"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDF2496525.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            31743..32249
                     /gene="folK"
                     /locus_tag="PC407_001068"
     CDS             31743..32249
                     /gene="folK"
                     /locus_tag="PC407_001068"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDF2496526.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDILFFN
                     DVASTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTVKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            32230..33015
                     /gene="aroF"
                     /locus_tag="PC407_001069"
     CDS             32230..33015
                     /gene="aroF"
                     /locus_tag="PC407_001069"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2496527.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLKILKAVKDEVGMAVITELMDVRD
                     IDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
BASE COUNT        13175 a         3519 c         5568 g        10834 t
ORIGIN      
        1 aatgttgatt taaaataaaa ataggatttc ggaggagttt attatataaa tgaaagatta
       61 ctataaaata ggagagatat ctaaaatata tggaataggt agagattcat taatgtatta
      121 tgaagaaata ggcattctaa gaccagttag agatataaat ggatatagaa tgtataatat
      181 atcagatata tggaaattaa atttaataaa agaatttaga agcttgaact ttccaatgaa
      241 gaaaataaaa gaatacctag atgacagaag tatagagtct acgaaaaata ttttaaatga
      301 agaactcgat ttaattgata aaaaaatagc tgaatttata agccataagg aaaatattat
      361 aaaaagattg agttcgattg aaagtgtaat tcaaaataca aaaatagatg agatagaggt
      421 tgtatatata gaaaaaagaa aagctttaga attaaatgct gatataaaga gggatgaaga
      481 cttcgatttt ttaattcaga aacttcagaa agaatatgaa gatagattta atatacttgg
      541 aaacaacaat attggttctg ccttttctac tgaagcaatt aataaaggta tatttaatga
      601 gtttaaatct gtattttgtt ttctggaaag taatgagaaa gtatataata tagtttttga
      661 tgaaggatat tatgtaactt taaattatac tgggagtaat tctaataata agatgtatat
      721 ggagaaggtg tttaaattta tagaagaaaa taattataag ataattgctg accctataga
      781 aatctataag atagatatac atgagactgg aattgtagaa gagtttgtaa cggaaattca
      841 agttcctata actaaatagc catatttcaa tggattagat ggtaaataga atgaaaaaat
      901 gttgatttgc caccgatttg ctactgtgtt ataaaatcgg tagcaaatta tattttaaat
      961 tataagatag catgaaaaaa taattgttta gggggttata aatgtgaaat gttctaattg
     1021 tggaagtgta aatattgaaa agggtatttt aacaactggt ggcatatatg ctgaaactat
     1081 tggattaaaa tatagaggag atggaaaatt agataaagtt ataaataaaa cttttccagt
     1141 acaatctact atatattcag atttatgttt agattgtggc gaaatagtta gaacctatat
     1201 aaaggataat actgatagga attggtgtaa agataagaaa tagaataatt tttgaagtat
     1261 attaatattt gaagttgaat tttattaaag atacaagtga aaaagtataa atgggaagaa
     1321 attgttatat ggaagaaaac tttgcatata ggggagatta gttttttaat ataataacat
     1381 ttttaaatgt agataaaact aaaaagggtt ctctagaaaa caatttctat ttaattatac
     1441 ttacttacca ataccatcac ttgatgtaaa caagattcta ctacattttc tccgtactct
     1501 agtataaaat catctagctg tgtatctata aaatcacaat aatcatataa actactaata
     1561 taagcagtta acatgtgtgt acatttatcc atttcatctc taaacatata tattactatc
     1621 cccctaaaaa actttcataa aacaaaaaga acaatatctt tttggcaact ggctgacata
     1681 actcatagtt tattttataa gcgttagtcc ctatagcttt gcgtcactaa atttctctag
     1741 ttttgccgat ttagttttat tctacaacta aaataataca ctaggataag ttattgttca
     1801 acatacttgt cagaaaattg aaatatttaa cagtatctta acaatatgaa atctattttt
     1861 cactcaagaa acaaactata catgtagaat atttaataaa aattatattt agttttgtat
     1921 tgaagttttt gtattaaaaa gtttagttca caagagttac ttggctagat aatagatgag
     1981 caaaaatatc ttgaaaaaat atatatgaat ggcaaaaaac aagggatgaa ttttatatag
     2041 actcatcccg taattattta tatattgacc ttgattaaat atattattat ttttaagctt
     2101 caatttttat attatatttc taaagtttct ataagaggat tatcgctaaa taattttaat
     2161 aaatttgttt ttttatatcc tttaggtaac ttagtatata taactacttc taaccaatcc
     2221 ccatcaaggc gtttaatatt catgtttata atttcaatat tagatttttt aaatttacct
     2281 tcaatatatt ctatggaatc agtgctgtta gaaattttaa gagaaacttg ctctgctgta
     2341 gggagtttca tccacttcct atctctatga agtgttatct gtacaataac aataatgata
     2401 cttgaaacaa taccaactaa gtataatcca gagccaatgg caagacctgt accggctgtt
     2461 gcccatatac cagcagctgt agtaagacca cttactgatt gatttctaat aaatattaat
     2521 ccagcaccta aaaaacctat accactgaca acttgtgctg caacgcgggc tgggtctaga
     2581 ccaatacctg gagtacccac tatatcatta aatccatact tggaaacaat gattataagt
     2641 gctgaagcta atgccactat aaaatgagtg cgtattccag cttcttttag tctattcttt
     2701 ctctcatatc caattaaagc cccacagaaa ccagcagtaa tgattcttgc tatatattca
     2761 aattgaatca atatatgatg gttcatttat ctagcacccc cttctatata atattatata
     2821 tactataata ctacattttt tatgtgaata tagtaaagtt taaaatttat ataatagtta
     2881 tatatgaata taaaatgtat aaacgtatat tatgtatata aaaatgatag aaagcatcta
     2941 attttaaatg attaaagagc aagcttaaat aaatcaaact tgctctttta acatagtgta
     3001 tgtttttatt tgtgatagtt agataaaaat ttatctgtgt tttttacatc tgcaaaactg
     3061 ttctgtagtg atgccatgaa tacttgatgt acagtatttg cattaatttc tatattattc
     3121 caagataaat tcttcgttgt acatgcatcg cttattagag ttatgtcgta tcgaagtttc
     3181 tttgctgttc ttacacttgt atcaatacac atatggctca tcattccaca gattactaga
     3241 ttagtaatat tttttttctc aaggatactt tgcaaatctg tttcaaagaa actatctggt
     3301 gtgtgtttaa ttataatttc ttcattatct agtggaaaaa caattttatt tatttctgaa
     3361 ccatgagtat taggaagaaa aaaagaagct gttttatctg ttgatatatg ctgtatatga
     3421 ataactggta aattgttttt tctaaaaaac agtagtattt ttttagcatt ttcagcagtt
     3481 tcttctgatt gaaataattc acattttcca tttttaaaat aatcattttg aatatcaatc
     3541 aatattaaag ctatagacat gattatacct tctatttact tttattataa tactattgta
     3601 aaaaatgtaa attacttact ttttggtatg tataggatta cttattgtag caaattcctt
     3661 tattatctag aatttcttgt tgaccatgtt caagcatata ttcgattcct atttcttgca
     3721 ttatttccac tgcttttaac atttttttac cacgattagt aagaaaatat tcaacatgta
     3781 atggatatcc agcagaactt attttatcaa taatcccaaa atcacgtaac tcttttaatt
     3841 gttgaatcaa cattttttga tttattccat aaatattatg ttgcaattca gaaaatgttt
     3901 tattagattg tcttaattca aatataagga tagttttcca ttttcctttg attatgtcat
     3961 gtacaatttc aagaggacat gtatattcag ctcttatttt catagcatat cacccccagt
     4021 aaaatgataa taaattatat cacttgtctt gtgatatgac aaatatatat gaactgtata
     4081 taaattttat gttttattaa gttctaaatt tcttctattg tagttcatat ttagtacata
     4141 aataattagt aagatataag taaataaata tataacatga ctatagaaat ttaactttaa
     4201 aataacttat gataatttag aggaggaata tatatgaata tatcaagaaa agctatgaaa
     4261 ataatagaat tagcacaaaa aattgcaaat aagagaggta tttcagtaga agaagcttgg
     4321 agtgaagcag taacagaata taaaaataaa tacgaacata ttgcatagat atttaaaaat
     4381 ttagaatatt tggtagaata taatgatttt aaatgatact ataaattata aggtatatca
     4441 ttattaacat agaaggagga tatctgcttg attgtaaaaa tagataatac tttagaaaaa
     4501 gaattttggc aatatgtatc acatgaagaa agtttaaatt tattcataat tggatacgtt
     4561 gagaattatg gatttagctc tcaatatcaa gatatatggt ctcaggtaga agatggaaat
     4621 ataacctcta taatattaaa aaataagtca acacttataa tttatagttt taaaaataat
     4681 tttgatatag gagaaatgaa gaatcatata aaagatttag atgtagaaag cataagtgga
     4741 aaaaaatgtg taatagatag actaataagt aaatacaaag atttttatga aaaactagat
     4801 aataaatttt gtgtattaaa agaaataaaa gaaatagatt tttccaacat gaaggagtat
     4861 aaaatagaaa atgctcagga aaaagatatt gatgaaatag gaaaactttt aaataggtca
     4921 gactataaag taagtaaaaa ttacatagaa gaaaggaaag aacatttaaa agagggaaat
     4981 gtaagagcat attttattag aaatgatgat actatgatta gcactgtgtc aactggtatg
     5041 gaaactagtt ttttggcaat ggtggtctct gtaagtacag ataaaagata taggggaaaa
     5101 ggacttgcaa gttatatggt ttacaatttg agtaaagaat tattgttgga aggaaaagtt
     5161 ccatgtcttt tttataataa tgatgtagca ggtaaaatat atcataatat aggatacaaa
     5221 gagataaatg aatggacaat tctttttaaa tagctataaa aataataaat tttcgtgtaa
     5281 ttcttaacgt gtattgtata atcgctcaaa tacttgaaag gtgtctagta gatgggtttg
     5341 ttagcattta tggtctcctt atctaagtaa agttcccttt ggaaatcggt gaaatactct
     5401 tttacatttc atgagtccta taaaatctat gacataagaa agacccagtt acattgagta
     5461 attgggtctt attatcatat atattgtgtg cttacatgat atagttcatt tctataaacg
     5521 tagtatttcg taagtgggaa ccaatttttt aacaagtgat tatttgaaca aagcaataag
     5581 gatatgctag cagatggatt ttgtataatt taaaaatcaa tatttagact ctatcatgta
     5641 ttttcatcat cataatccaa tttgaaattg tatctaaaac cagtttgcta tttccacagt
     5701 tacagtggtt actagcttgt tctgcttctg tcataagcct aaatgtaagg gatcggacat
     5761 tgttgagtaa atcaatctct ttaccaacag ttctataatc aataaaatga tcttgtgttg
     5821 cacctaaaat caaaatgtct tggtttatat tttttgcaat agaatctatt tgataatact
     5881 tgattttctg aaaatattca tagggtgtag acgcttcata tgcgtacatt ccatgtttta
     5941 aaccccatac caccatacta tcgccacttt tcattttttg ctttattata aaattgacta
     6001 gccattttgt tttgagtttt agcagtagtc taagtagatt ttgcattttt attggttgtg
     6061 aaccaactat tatgtctaag aaattgggga atacagacca agctaccacg cgttcaattc
     6121 gtttttcgaa ggctgctgct cgtggagcaa gaaaacctcc caaggaagca cctacaatag
     6181 ttatattctc taaatgaaaa taatctaaaa ttgtcttaac gggacgttcc cattgataag
     6241 taaagtgttt tccctgcaaa cgcatcactc caccttgacc aggtccctca aaaagataaa
     6301 tatcaaatcc ctgtgacgca agataaagca ttgggaaaaa gaattcttca aagtaagaat
     6361 catttccacc gtggagcaat ataacatctt ttacctcgcc ttgcgctttt gcatacatta
     6421 caggtagggt gatattttca tatggtacat gaaaaatttg tacagttcca tttgagaaat
     6481 attcagagta gtagttgtag aatagagtag ttgctttttt gtagactgat attttatcag
     6541 ggtcaccatc atacataaaa aattcactca tacgatagta agcaattgca ttttctgtgc
     6601 gtccatcttg ttcagcttct tgtgccagat acataagttc tgttttccaa ctttcgctat
     6661 cgacaatttt agaggaaact ttttgtatat cttccaagcg ccccccatcc cacataataa
     6721 tcctgtttaa ctgaaaatta aaattggcat ttgtattaag gtaatataga cctttattaa
     6781 aagaaatttt atttgttttc aatgtcatca ctcctaaaaa gataatgcat agcaataaag
     6841 agtaatcttt aatgctatgc atttattgta acaaatatta gaagtactga ctttcaaata
     6901 gacgctattg gcggataaaa tatattttac attttcctat aagctcactt gcagtaatgc
     6961 caagatattt tttgctggaa gctgagaaat gagacggtga actaaaccct gcgtccatag
     7021 cagctgaggt aatatctcca ctttttaaca ataactcata agccttgcga agttttgata
     7081 aagccaaata gctgtttagt gatactccca tttgttcttt gaaaagatga gaaaaccggc
     7141 ttttggacaa ttttacttcg gcacatatct caaaaaagat ttcattgtga atgtgtgtgg
     7201 actttagaat atgattcaat gcccattgta ttctactgtc tgtaatagac gaaatatgat
     7261 aatttaaatt caatagacgg aaagcttgtt ttgaaaaatc aatatagctt tgggacaatc
     7321 cttttacctg acaagctgta taaatatttt gtattcgttc aactataaga ttatctataa
     7381 cgaaaaaaga gtgatttagt aattggtgtt gactaatatt ttgggcgagt atgcttgtat
     7441 catctatcaa gaaaaccaac attttattat ctttacttga caccgtatgc gatacattgg
     7501 aatcaataat aattccttta cattgtaatg attcgccctt atcaaacgtc acagataagt
     7561 taccagactg tgatataata atatgttttg caagatgttt atggatagtt ggttgtctat
     7621 actctgtatc taaaaatata tggtcaatca ttacacaaat ggtagtcata gctgtcaact
     7681 ccttgttcat agcttagcat attatacagt ttttaacaag aaagcttagt gattacaagt
     7741 atttaaaaat cctattttag gcaatgaaaa ataaaaaact ctatcgagaa ttaaagtatt
     7801 tataaataac tatattttta ctatgattca tgatagagtt ttttaaaatt tatgatacta
     7861 ttttttatta tttaaataat caagtgtttt aagtttataa ccttcttttt cccaatgtgt
     7921 aagaacctca tctaatatct cagtatttgt tgatgaattt ggatgcagta aaactattgc
     7981 accaggatga gttcttgaat aaattttgtt tttggcaaat tcatgagtag gttgtttctt
     8041 ttcataccaa tctacataag caaaactcca aaatattgat ttatatccta aatcttgtgt
     8101 atattttaaa gattgttcgc taaatttacc cattggaggt ctaaaatact taggcatttc
     8161 ttttccagta acatctttgt atgctttttc tacacttgta atttcttctt tgaatttttc
     8221 aaaatctgta atacctgcca ttgatggatg gctttttgaa tgattacaaa ctaaatgtcc
     8281 ttctttttcc atacgcttta ttaactctgg attgcttttt atataacttt caactacgaa
     8341 aaattgagct ttagcattgt gttttttaag cgtatctaat aattttggag tgtttccact
     8401 ttcatatcca gcatcaaatg atagatatat aactttttca ttgggatttc ctacataata
     8461 agctccgtat tttttaaaaa aatcagcttc ttttataggt gatggctgtt taccatcctc
     8521 cctagggtta aaataccaat catattcatg tgtgtcgagt gttggactag aaatttgagt
     8581 ttttgtttta tctaatgatt taaaatttat actagcaatt ccaaaaagta ttagagcaaa
     8641 tgctcctatc ataatgtatt tttttaggct atcttttttc acaatttcac ctcttattta
     8701 aaatttaaag tatatttctt ataatatagt ttgaatattt atagttaaaa atattcacat
     8761 atacaaataa taaatgaaag aagtttactg taagatataa agttgatatt aattatctta
     8821 aataaagtgt atataaatga taaaatataa agagaaatga ttgtaaataa gttaggctgg
     8881 tttaacttga agggatataa aaatggataa tataataagt gtattgaatg atgttgtttt
     8941 tttagatata gaagttagtg gattagattg tttaaattca gaaattttag aagttggagc
     9001 tgtcaaggtt aaagactgga aaatatatac ctatgaatca ttaataaaaa acaaatttga
     9061 ggtacctata gaagtattct cagtatgtaa aaatttagat aaaaatgatt tagaaatagc
     9121 taatgaaata gaactggtgg aagatagact tgtaaatttt gtagaagata gtttcattat
     9181 atgtcatgat ttaagtctga aaaaaaagtt ttttgagtat catatgccaa agttaaaaaa
     9241 caaatttatt gatttaattg aattagcagt tattttagaa ccatatcata aagattatag
     9301 cttagaatac ttaaaaaata ctttaacaaa ttgtaattct aaagtggaaa atagagcttt
     9361 atcagatgct attgatataa taaatattgt aaattgcctt ttagtaaaat ttaataacta
     9421 tgaaaaaact actttggagc cattgagttt taaaataaat tcatatctta aaaaatttaa
     9481 tcttccaaca tgggaatgga gcaaattttt agaagaggca aattatgatt tatcaaataa
     9541 tataaatata aaaaaagaat ataatatatt tgactcaaaa gaagaaaaga agaaggaaag
     9601 agaaacctta aaaattttaa atgaagaaga aaaaaattat gaagaattat taaaatacaa
     9661 aactatttgg gagaataaag aaggatttac atatgaatat agacctggtc aatatgagct
     9721 tactaaaaca ataagagagt tgtttagaaa tagcgaagat gaagaaaaaa ttgcatgtat
     9781 agaggcacca actggtatag gtaaaagtgt aggatattta ttgcctgcaa tattagaggc
     9841 tagaattaat aaaaaaagat tattaatttc tactgacaca aaggaattgc agatacaatt
     9901 aataaataaa gatattccaa atgtgttaga ttctttgggt ctaaatggaa aagtaagtta
     9961 cggatatata aagggaaaaa ataactatat ttgtatagat agactggaag catatataga
    10021 tgattatgag tctcaaaacc ctactaaagg agaaatttta tctttaatat ttttgaagag
    10081 attagttgaa ggtggcaagt atggagatat agaagaaata aattatttag tgtttgacaa
    10141 cttcaaggaa ataagtaccc acttaagaaa tgtaagttgt gacccaaata tgtgcagacc
    10201 taaaaaatgc aaaaaagatt gtttatataa aaacagaata gaagaattaa aagaagaaca
    10261 tataacagta gtaaatcatt cactattagc taaatggccg tataaggatg aaaaaccttt
    10321 agaaaatata atagtagatg aagcacataa tttgactgaa aaaggatatg atttcttttc
    10381 aagcattata aattcaaagt ctttaaggta tttattacaa gaaatttatc cttacgagtt
    10441 tattcaaaat agtagcttta tatataaaaa atatagtaga aatatgagaa aaataaaggc
    10501 ttttgataaa ttttataatg tattaaaaat tggaagagaa gataaacaaa aaattgctag
    10561 aagtataaat ctcattattg aagagataga ttcaatatta aagtttggta attgtaatga
    10621 atataataat gtaagtaatt ataatttaag atgggaactg aatttgcaaa tagatgaaat
    10681 tgtgggaaaa ctaaaaaagg atggtataga tactgaaata agttatagag cctatagtga
    10741 aaagataaag ttgagttgtg agaaaataat aaaaaattta gtttcaataa taataataat
    10801 ttataggaat attgatgatg atagtataga taaagaagca gatatatata aatttggaaa
    10861 ggcaaagaca agagatttag aggatataaa gattatattt gaaatctttt tagagtatga
    10921 tgaaaaggat gattatgcaa ggatagttga aattgataag aattataatg actttgaatt
    10981 tcgagtagta ccattaaaaa tagcagattt atttgaagaa aatattttat ctcaattaga
    11041 aaaaggaata tttttatcag caactttgag tttatctgaa agtatgtctt attttaaaaa
    11101 tacattagga atagacagag ttaagaatgt ggaaaagatt atagaaccaa tttttgatta
    11161 taaaaataga gtctctgtag ttggattttc tgatatttgt gagtacagaa attctgaatt
    11221 tccaaatgag atgagtaaaa ttatatctaa tatatcaaag attacagaag gtcatacatt
    11281 agctcttttt aatagtaaag atagacaaga aaaaacttat gaaatattga aaaaatactt
    11341 acatagtttt aacttagaaa tttatgctga taaaaagggg attagacacc ttaacgattt
    11401 aaatagaaaa tgtgtagtac ttggctcaaa aggatgtttt gaaggcgtag acattccagg
    11461 ggatggtttg gtttgtgtaa ctttagacaa gcttccgaat ctaaatccaa aagacccttt
    11521 gtattttact ataatgaaga agtatggtat agactactat actataaatt atcctcaaat
    11581 gaccataaag gtaaagcaag ctatggggag aattttaaga agcaaatatg actatggatg
    11641 ttttgtgatt tttgatgtag gaactaatat aagtgtttta aagagattag agaaagattt
    11701 acacgattgt aaaatttcaa aggttaatag caatgagttt tatacttata taaggcgaca
    11761 tttaaataaa tctagaagtt taatactaaa atctgttata tttgatacta taaaagcgtt
    11821 gaatgttgat gctaaaatgg ataacaatga tgttgataaa gatattataa aaaaagatat
    11881 aaatgaaaat ataagacaaa gagctgtaaa aggagaagtg taccatattg atattataaa
    11941 aaaagatatg aaagtaaaat attttgatag aaactatctt ataaatttag atatatttat
    12001 gagagaagaa gataaaaatt aagagtggga gtgagatatt tggcaaaaca aaagttttat
    12061 gctgtgaaaa aaggtaaaaa tataggtgta tataatacat gggatgagtg caaaaaacaa
    12121 gtaaatggat tctcaggagc tgaatataag agtttttcta cttttcaaga agctaaagaa
    12181 tatattgatg ggtcagaaaa attgagtttt caagaagata aggagtttat agaagcatat
    12241 gtagatggaa gttatgaaca ttcagtaaaa atgtatggtt cgggtgttgt gattttaaaa
    12301 aataatgaag ttataaaaac ttatagtgaa aaaggaaaag agaaaacttt agtaagtatg
    12361 agaaatgtag ctggagagat agaggcttct aaaattgcaa tgcagtactg tattgacaat
    12421 aatgttcaaa acttgatttt atactttgac tatgaaggga ttgaaaagtg gtgtactgga
    12481 gtttggaaaa caaataaaga aggaactata gcatataaaa atttctatga cagtattaag
    12541 aataagctta atgtaaaatt tacaaaggtt aaagctcatt ctggaaataa gtataatgag
    12601 gaagctgata aattagctaa aaaagctatt ggtgtataat aaaaaaatat ttatacataa
    12661 ttacatggta taagagtata tctaaattcc tactttataa ttaaacaact tgtaattaaa
    12721 caacttgtaa ttaaacaact tataattaaa taaattttga ttaaaaaagc agattgtatt
    12781 atgaaaaaat aagtatctaa tttataaaca attaataata gtattaatta gatacaactt
    12841 atttaatata ataaaatctg ctttttatat aactataaaa tcttcctaca ttctattaga
    12901 attgcccata aataccttca agttcttctt ttaatacttc agggtcattt ataccaatag
    12961 acatggttct agccagtata tttgcttggt cataagttga tgtccatgag aatacaccac
    13021 ctagacaatt tctaagaaca tattgagttt ttaagaatat tgataaagca tcatcatatg
    13081 acattgcaaa atctccatcc ttaactaaat acggaacttg agcagtatta tcaaatctat
    13141 attcatatcc atttttattt atataatccc ttctaagctc atcataagtt cttgttatag
    13201 tggcacctaa tcttccataa aatggtatac cgagaaggat tttttcagaa ggcattccag
    13261 cattctcaag atttctcacc attgcatcaa cactatatcc tggcaaagat aagtctgaat
    13321 caaaaagatt tgcttgatgt tttctaccat ttgggcctgt ttcacctgct gtaaaatcat
    13381 aactcataag attaaaataa tctattatag gagctatttt atctatttca gcacttgaat
    13441 ttatatatcc tctatctcct gttccagcta cactaagcca tttatcatct cctataacat
    13501 ctcttatggc agttagtaag agtgtaaagt tttctctatc ttgaggtctt gatgttattc
    13561 cagatgcact acttccagga tattcccagt ctatatctat tccatctaaa gcatattcat
    13621 ttatcatctg attgacctgt cttgcaaaat tatatctaga tgtaggtgtt aatgctgcat
    13681 cagagaatcc ttcagctccc caacctccaa tagctacaat tacttttaag ctaggttttt
    13741 cacctttaag agcaagtaat tgatttaaaa atctaggata aggaataaac aaatttccag
    13801 actggtctat ctcagcaaaa gcaaatataa ctgcatctaa tagttgagca tctacattag
    13861 ttggatttcc aagaacatat tctgctacaa ttggatttat atctggacag tttgcatttt
    13921 caactattgg attgtatggg tctgttattt gtggtctaac taattttttt gaatctttta
    13981 aagttttcat agctattttt ttagtatttt tttcgctagt ataatcgtag tttttataat
    14041 tatgtttata gaaatcttca taactactat cttctttgtt atagctagaa tttattttgt
    14101 cgcaagattc atctttatca tagttaaaat tcatttcatc acaagactta tctttaaact
    14161 tatcttgttt ggagttgtga actttgttag tatttttagt atgttttttc tcatggttgc
    14221 aatcatcttt aatatcagtc ttgtgttctt gtttacagtc aagagctttg ttcaggtagt
    14281 catgatgttc atgttcacag ttacattcat tttcaatgtt tttagtatgt tcttttttcc
    14341 agctacaagt attatcaatt ttcttgctca cttcttcatc attataatta tctggtacaa
    14401 aacataagta ccagtccata catgaatatt tcttattaca actgttaact ctatttttta
    14461 attccttata gtttttagga taaggtaaaa tcactggcat tccaggaaac cagtttgcag
    14521 gagtaactat attatctcta tcactagtct gaagtgcatc tactattcta agtatttctg
    14581 gaatatttct tcctgtagtt agtggataat aaagaatcgt tcttagaatt tgattattat
    14641 ctataataaa tacagagcga acagttgatg tatcactcat tggttttgat atcatgccgt
    14701 ataacttggc aattctcata tctctatctt ctataatagg aaatggaatt tctacacctg
    14761 taagtaaaga aatattatac atccaagcta aatgtgaact gttactatca acacttagac
    14821 caattagttc tgtatttctt tttttaaatt cgtcataata tttagcaaaa cataaaaatt
    14881 ctgtagtaca aactggtgta aaatcaccag gatgtgaaaa taaaacaatc caattaccct
    14941 tatagtcaga gagtctaata ggaccatttg ttgtattggc tttaaaatca ggagcctttg
    15001 accctaaact tggcaaattt ggcatgtaaa tcacccctca ataaattatc atttatttta
    15061 ttctattaat taatacatat tagtgttact attttattaa aagttaagta atccttatgt
    15121 caaaaaaaga tatttattaa aaagatttaa tattttccta atataaatga caatagtaat
    15181 tgctattttt ttactaattt ggtatagtaa tggataaggg gtagtgtcaa ttttagtgat
    15241 tatacgaggt gattggattg aaaaaaattc taagaatttt agttatctcc atgatgatat
    15301 gttttgcaat gacttttgca ttagtgttaa taaattcagt tatgggagta catataggat
    15361 tggattatta ctttttgatg ggaagtgtgc ttacagtttg tttaacaatt ggaattgttt
    15421 tatgcaatgc tagaaaaata tttgatagca aagctataca caagacaaga acgaaaactg
    15481 tcagtactaa taaaaaacaa aagagtcatt ctagggatat aaaaagaaag atatcataat
    15541 ttatgtaaag gaaatattta tgtataaaat aatgattgat aggtaaaata aaaacacgat
    15601 ttaacttgtg tttttatttt ttttcaagaa ttatgatata ataaccaata agaaatttag
    15661 tttaccataa aatataaaga ttattaaatg gagagggaac tataatgaat tttagagatt
    15721 tatttgagaa ggctgtagat tttattgatg aaaaaaggaa atctatagtt gcgatttcat
    15781 tatcaacatt aggtgtaatt gctttgataa tagtattttt cttaagtagt aacgagttta
    15841 gtgtaggaaa tgaagctaat gagttgttaa agattataga gaaaagacag tattctatag
    15901 ctgttgatta ctacacaagt atagaaaaaa agttttctga ttcaaaaatg gaaaggttta
    15961 ataagtctgt ttctaaaaaa ataaataaat tgctattaaa tagtggtgat aaatatttag
    16021 atggagacat aagtcaagaa agttttattg gattaataaa tactgtgaaa gaattaaaca
    16081 aaataagtat agatgtggat gacttattag ctcaagctga tagagttaga gaaatgtata
    16141 aagaagaaaa tactacttat gatattgcca ttaactacat aagtactgtt tcaatattaa
    16201 atggtatggg tagtaaccta gatgtataca aacagaatat agaaacaatc aaagagtcta
    16261 gagatgttta tgactcagca gtaaaagacc aaaaagcata taagtattat gaagctattg
    16321 aaaagtataa taaagtatta aaagaagatg aaaagtatta cagtatggct caaaatggga
    16381 aagaacaatg tattgaagaa atgtatgatt actatataag tagagctgaa gaggcaaata
    16441 cttctggaga ttatgaaaga gcattacaat atattgagta tttaaaagaa gattattcag
    16501 atgatgaaaa ggtacagagt ttagaaaaaa aatataaaaa gaatttatca ttgtatacct
    16561 taacatctga tgacattata aatgtaatag ctaaaaaaag tggcaaagac aaggcaaatc
    16621 tatctataaa ttcacttcca caaatgataa aaaataataa atactattat gctgaagtat
    16681 atgagtatga taaattaata aatgaagttt tggtaagtgc aaaaactaaa gatttatatt
    16741 cgtataaaga tggtaaaaaa gattacaaag tagattatgg tgatggatac tttagaatat
    16801 tagaagatgg aagttatcaa tttggtatta caaaagataa agctaagttt gtgttaacta
    16861 atactcttga tgaaaaagaa aataagtata aaaagattga gatattagat atagagaaag
    16921 cagatagata tgtaaaaagt aaaaagagtt tagaagaatt gtttggaaaa tataaaaata
    16981 tttattatta tgctgtagta aataaaggct tatttagagg caaagaagtt tatgctatta
    17041 atatttacaa tgaaaaaata tatgccatat cagaaaatgg actaaatgaa tattaattaa
    17101 taatattaaa ttaatatata attaaaaagt ttttggggtt ttaaggcgca ttatgtagta
    17161 atataataat agggatatat aaataatttt attgtaagga gaagtatggg atgagtaatg
    17221 tgaacaaaaa gctagttata gctattggag ctgtagcatt agtagcaata atttttatag
    17281 gcataatgtg catgcaattt aaaggggaga aaatagcaaa aaacacatat gttaatggag
    17341 tagatattgg aaaattaact aaatcgcaag caaaacagga attagctaaa aaatacaaat
    17401 tagagaatgt tgagtttaat tacaatgata aaagttggaa agtaaagagc aaagatttaa
    17461 atttatctac tgatctggat aaaacagtag aaaacgcata taatttaaat agaaagagta
    17521 gtttctttgg caatctatca aaaactattt caacaaattt tggcaaaaaa agcaacttag
    17581 tagttgtgat aaattatgat aaaaataaat taaaagcaga aatggaaaaa atagctaaag
    17641 aaatagatgt agatgtaaaa gatgctacac ttgatataag tggtgaaaaa gtcaaggtta
    17701 taccagattc tgatggtctt aaaatggata tttctaagag tatggagaat tttgataatc
    17761 aaactaaaaa aggtaactat aaaaatgagt tagtagtaaa agctactcca gcaaaagtaa
    17821 aaaaagagca acttactaat atagatacaa atttaggtac gtattcaaca atatttaaaa
    17881 cttctcaaat aaatagaagt ataaacataa aattagcaac agataatata agtaatgtat
    17941 tacttatgcc aggagaaact ttctcattta ataaacatac agggaaaaga agtaaggaaa
    18001 atggatataa aagtgcacct gtaataatgg aaggtgagat ggaagaagat tatggtggag
    18061 gagtttgtca agtttcatct acgttataca attctgtgtt atacgcaggt ttagaaattg
    18121 ttaatgtaaa aaatcatact ataccatcaa gttatgttcc aaaaggaaga gatgcaactg
    18181 ttgcagatag tggaatagat ttcttattta aaaataatct aaagcatcca gtttacataa
    18241 aaaattacgt ttcaggaaat caaattgttt gcaatatata tggaagtgca gaagataaac
    18301 aaaatataac gatatcaaca aaattagatg gcgtttcaca aactacaatg aaaagagtta
    18361 atgaccctac aatgcctaaa ggtaaagaaa aagtagacaa aagtggaaga aatgcatatt
    18421 ctgtatcaac atatagaaca tttaatgatg caaatggtaa aaaaattaaa actgaaaaaa
    18481 tagctaattc atattatcct aagaaggaag gtattatatt agttgggact atggaaccaa
    18541 aaccagagga aaaaccaaat acagacgaaa ataagaataa tcaaaataca aacaatcaaa
    18601 atccaaataa tcaacaaaaa ccagaaacac caccaactga taataagcct aatgagacac
    18661 aaccacaacc gcaagcttaa agttctcaag aataggattt atgtagagta ataaaaaata
    18721 taaatctaaa taggagggat tactttgaat ttaaataata taacgaatta cgttcaaagt
    18781 atttgcgaga atatttctaa tgtattggat gtagatgtta ctttagttac aaaagattta
    18841 actaggatag caggtacagg aatattcaga ggtaaaatag gagagacaat atcagagaag
    18901 tcagtgtact ctcttgtttt aaaagatgga aaatcctatg ttataaataa ggaaatagaa
    18961 gagaactgta ataaatgtgc aaatagagaa aattgtaaag aattagctga tatatgtact
    19021 cctgttaaaa tgggagaaca tatacttgga atcttgggta tagctgcgtt taatgaagag
    19081 caaaaagaaa agatactttc aaaagataag gagttaactg cttttgtaag gagtatgtct
    19141 gatttaatat cttttaaact agatgaactt tataaacaag aagttaagac tatagatgac
    19201 cttgagaggg aagctataga aaatgccata aagaaatatg gaagtaatac agaaggtatg
    19261 aaaaaagtag ctgaggcatt aaatataggt atagctacac tctatagaaa agtaaaaaaa
    19321 ttcaacatta aaagttaata gtattgaaaa agaggtaacc tttttatgaa aagaagagga
    19381 ttaccttttt tattatgaga atggtggaaa aatatgtata gttttgactt gacatattaa
    19441 atatgttatc ttaaatatat attttagagt ggaggtgagt attattttag ttaaagatat
    19501 aaaacaagac atagaaagca tgcctagaaa aattttagat atatctttga tagaagataa
    19561 taaaagtgat ttgacttttt ttaatataga agaagaagac ggtgatgaaa cattaaaagc
    19621 attaaaacag agttatgtga ctacaaatta taaagaagaa actctattaa acttgcaagg
    19681 agctttaaat tcaaagtcag acacaacgtc taaggagtta aaattagaat catataaaag
    19741 atataacaat gcattggatt tagcatataa aaattatata actagtgcca ttaatatggt
    19801 taataaagct ctggagataa atcctaaaga tattgatata ctaaatctta gaggtctttt
    19861 aaagttatta aaatgtgatt ttgctaaatc atttgagagt ttttatactg ctttatgtta
    19921 tgagaataat catatatcta gaaaatatgt aaatctaatt tcatctgaag attttaaaac
    19981 ttttttagga agatacaatc attctataag atttataaat gaagaattaa attatgagtc
    20041 aatacatatt ttagagaaca taattgaaga agagcctgag ctaatagagc catatgtaat
    20101 cttatctttg ttatatgata aactaggtaa tgtaaaaaag agagaaggat atttagacaa
    20161 acttaaagag ctagataaag ataatcctgt atttgaaaaa aatgaagaag aaaaagaaga
    20221 tacaagtaaa aaggaagaaa taagaaaagt aaaaagaaag aaaagtattt taccatatat
    20281 tatagttggc tgtgttttaa taggtatggg gatatatttg attcaaagca aaaagagaat
    20341 agaaaacttg aataaccaaa taagtagtaa agaggaaaag ttaagtgaaa ctgataaaaa
    20401 gttaggagaa acgagtaaaa aattagaaaa aacgaataag gaattaaatg aggttaaaaa
    20461 taaagaacca gaaagagaga taactgtagc tgatgaggaa gatttatatt atcaagctct
    20521 aaatttaaag aaaaacaaag aatatgaaaa agccatagat aattttaaaa gtgtagtttc
    20581 ttctggtaaa actaaaaaat atatttctga gtcaatatat caattagcaa taacaaataa
    20641 attattagga aataaggatg aagctataaa atattacaaa aaatatataa atacttatac
    20701 taaaaatgac cagtattatg atgattcata ttacgagttg ggtatgcttt attatgataa
    20761 tggcgattta aaaaatgcac aacagacttt ttatagtttg agaagtgaag ttccagacag
    20821 tatgtataac aattcaaaaa taaaagaaat cttaagtgaa aaataggttt atatagttaa
    20881 gttttttgaa acaaccaagt atgtatttta ttaaattaaa ataaaaataa atttaaaaag
    20941 agattgtgtc aaattataac tttctatttt aaagaaagat atttgcttgt tgataaaact
    21001 acaaatatat gatttgaagc aatctcttta atttttggag gtggaaaatg aaagttttca
    21061 atcaattagt aataatttta tctatctggg taataggaga gtatataagt tcctttatac
    21121 aaggaattat acttattcct gggagtatag ttggtatgct attattattt ctactactac
    21181 aatttaaagt tttagacttg agtagtatag aaaatgtaag tggatttttc ctcgataata
    21241 tggctatatt ttttattcca gcaggtgtat cacttataaa gtcattagac ctaataaggg
    21301 aaaatgtatt agtgctttta ttagtcatat gtttaagtac actaatagta atgtatacta
    21361 ctggaattat tgtggagaaa atgataaaaa agaaggaaga aggacaaaaa aatgtataat
    21421 gtattacaga caccagtatt tggtataata gtaacaatag tttttttcaa tttaggaaga
    21481 tacattcaac gaaaaacaag taatcctata tgtaaccctt tattaattgc aatcgtagga
    21541 ataatattgt ttttaagcat atctaaaatc ccttatgaga attacaagat aggagctgat
    21601 agtctaaatt tctttttagg tcctgtcaca gtagtacttg cagttccact ttataaacaa
    21661 tttgaattat ttaaaaagca tatgtttgag attattgtag gaataggttg tggtatagtg
    21721 atttcttttg tatctgtatt gataattggc aaaatagcta attcagatgt aagtataata
    21781 aattcactaa ttccaaaatc tataacaaca ccaatgggaa tatctttagc aaattcttta
    21841 aatggtatag aatcaataac agttgtagcc ataattttta caggtatttt tggtggtatg
    21901 gttgcttcta cagtattcaa actggggaat ataaatcatc cagttgctaa aggaattgct
    21961 cttggaacat ctgcacatgc tttaggaact acaaaagctt ttgaacttgg agaaatagaa
    22021 ggagctatga gtggtgtttc tattggagtt tctggtacaa ttacagtaat actaattcca
    22081 attataatga attttatata aaaaataaaa ttaagacgag taccttgaat acttaaaaca
    22141 aaataaaata aaaaaaattt aaaataaaaa ctgaacgaaa aaaatgcaaa taatgaaata
    22201 aaaaaatcat gtactgaaat tataaaaaga aaaatttcat ttaggaatta attataaaga
    22261 gataattact aaacttgaat ttgctaaaat aaaataaatt actgcgaaaa tagatattta
    22321 gaaaaaaata ctataaattt actatattgc aagttaaata ataagcagta tcatattatg
    22381 ctagaaaaag tttcatttaa ggcataaatg agatgatttg aacgttttta aagattagaa
    22441 tataaaagat tgcactagac atatatgaaa aagataatat aatagatgat gtaatagatg
    22501 acagttcaag aaattataaa aaaaatttat ttgaaatatt atctattaca tcaggaataa
    22561 tatatgtaag agtaagcttt taaaattcta aagatttaga aagattgaag ggagaaagaa
    22621 atgagtgaag ttaagaatac cctacaaaaa catccagttt taagatattc aacaatgcta
    22681 tttgggatgt tgatgacatc tataggaata aatgggttct taagaccagc acatctgctt
    22741 agtggaggtg ctacaggtat agcaacatct ataaactatt tgacaaatat aaatgtaggt
    22801 ttattaacat ttttaatcaa tattccaata tttatcttag ggttcatata ccttgaaaaa
    22861 gagttttgta tttcaagttt ggtaaatatg attgtatttt cattattatt aggagcaact
    22921 caggaaataa gtaatattat acctatacat gatatattgc ttcaaagtgt atatggtggt
    22981 atattatctg gtttaggagt cggagttgta tttagaacta ggtcttctca aggtggtaca
    23041 gatataatag ctgcaatact aaaaattaag aagaatatag aaatgaaaga cactgcttta
    23101 gcaataaatg gcttgatagt acttacagga agttttttat ttgggttgga tttagcatta
    23161 tacactctta taggtctatt tctaaatgcg tattcaatga gttttattaa agatgcaatg
    23221 aattaccaaa aatctgttat ggtaatgtca aatgaagtcg atttaatagc agaagatata
    23281 atgaagagtt tagttagagg tgtaactttt ttagatgcag aaggtgcata cactcatcaa
    23341 aaaaagaaga ttatatatac aatagtttca tcaaatgaga taccaaagat aaaagatata
    23401 gctttgaagt atgataaaaa ggcctttata tctgtaaatg atgttacaga agtaaaagga
    23461 agagggttta aagcaaaaga tttatagaaa attttacaag ctgggagttt ataatgtaaa
    23521 tttgtatatt atatctctca gcttatttaa atttagtata tgaaaataaa ggttttatca
    23581 atgatttgtg tatcacttta aaagatatca ttttaaaatt aaaattgaaa aaattactca
    23641 atatgtagaa aacataacca ctattaggat ataataaatt taatagattt agtaaaatat
    23701 ctaggaataa cgttggagga atagcattat gaatttatct aaagaatatg ttaatttgta
    23761 tgataattat ttgaaatata taaatgaagt aaacaaagag attagaagta taaagaacat
    23821 gaagaaacgt gttttaaaaa agggcgattt tgctccagaa aatgaagtat tagaattgga
    23881 aggaaaaaca atacgcgata tagatgatgt cgatacaaaa aaaccaaaaa ataaaatata
    23941 taaattttca aatggggatg tgtatgtagg aaaattttta gaaggaaaga tggaagggca
    24001 aggatcatat actttttttg ttgatgaagg aacggctatg gagtacatag gagagtttaa
    24061 agaagacaaa aaaaacggaa aaggaaactt tgtattttca aatggaaatg aatatatagg
    24121 ccattttgag gaagatatga tgaacggtat tggaaatatg ttatataata gcaaagatga
    24181 atatattgga acttggaaaa atggaaaaaa agatggaaaa ggtatttata agtggagtga
    24241 tggatgtata tatgcaggtg aatttaaagg tggaaagatg gaagggtatg gtatttgcta
    24301 taatagtaaa ggggaagtac tttatgaagg agaatggaag aataatttga ttcatggtaa
    24361 aggaacttat atatgggaaa aaggtaaaaa gtatataggt gaatttatgc atggtaaaaa
    24421 acatggacaa ggaacttttt atctaaataa tgaattggtt tatgaaggga cttggaaatt
    24481 tgataagcca tccatcttcg acagaacatt agatgaaata ttttcattaa gactatagct
    24541 ttatctatta atagataaag cttttttcta atacttactg taaataagta tgaatgatat
    24601 tgattagtat acactatcaa tattaactca ataaatgtga tattaaaaat ttttgattga
    24661 ttttaagaag gagggatata ttgaagagat taattgcttt agtgttagta atacttattg
    24721 ttttatttta tagccctgaa ttgatagaag aaggatatga tttaaaagaa aaattttttc
    24781 aaagtgaaat atggaatgaa tttttagaca aaataaatat aaataataat tctaagaaag
    24841 ataaaaaaga acaaaatcaa aatttacaat caaataatac tgaaggggag aacttaggag
    24901 agtcagatat aaagaatttt gaaaaaataa gcttaggaga taatttaagc tatgttttat
    24961 caagtattgg taaacctggt agaattgata ttagtgaata tggttttgat tggtatgtat
    25021 ataatcaata tggaaaagaa tttgcaatgg tcggacttga aaatgatgaa gtagtagcac
    25081 tttatagtaa ttctattaat tcatgtgaaa atcaagatat aaaactaaat caagatagac
    25141 aaacagtgag aactaagatt acaccattaa aatataagag aaaaggaaat accagatata
    25201 taataaactc agaaaatcaa tatgatataa taagtaaaga aggtaagtat attacaattt
    25261 tctacgatat tcatgaagaa aatagagttt gctcatattt aataattgat aaaagtactg
    25321 aagatgagtt taaaaaatta tatccagatg atagagaaga acttaaaaaa tgttttgaac
    25381 ttgaagttat agaccttgta aatagtgtta gaaatcaaag aggattaaac tcattgagat
    25441 acagtgaaca ggcaactttg agttcaagaa aacatagtga agatatgaga gataataatt
    25501 tttttgacca tataaataag aagaatgaaa ctccatttga cagaatgaaa agagaaggta
    25561 tagtatatac ttcagctgga gaaaacatag cagcaggaca gattaatgca atatatgcgc
    25621 atgaagcttg gatgaactct gaaggacata gaaaaaatat tttggggaac tataataata
    25681 ttggagttgg agtaatattt ggaggaagct ataaaacata ttacactcaa aatttttata
    25741 aataactaaa atatttgaaa ggaagttaaa aaatgacatc atggaaaaaa aagacagtat
    25801 ataagtgctt aatagcagta gtcttgtttt gtggaatagt gttaatatct aattttagta
    25861 aagtttctgc tttgatgatg gatactaatg gaaatgtttt gattaaacat ggttcaaggg
    25921 aaaaaaagct aatagcaatt acctttgatg atggtcctca tcctaaagaa actagtcaag
    25981 ttttagatgt attaaagaaa tacaatgtaa aagctacttt ttttatagct ggaaaacatg
    26041 caaaatggta taaagaacca ttagtaagag caagtaaaga aggtcacgag ataggaaatc
    26101 atactttcaa tcatcctgat ataagtaatt taagttctag tcagatagaa gaggagattg
    26161 ttaagtgtga agacattttg aaagaagtaa ctggaaaaaa acctacatta tttagaccac
    26221 catttggaag ttatagagaa aaagacttaa tagaaattgc aaaaaagcat gactataaag
    26281 ttgttttatg gacaggtgta gatgtgaaag attggaaaaa tccaggagct aatagcattg
    26341 cagataaaat aattaataaa gtaaaaaatg gagatataat acttttgcat gattatgcaa
    26401 ctaatgatac agttgaagct ttagatatgt ttattccaaa aatgattgaa aaaggtttta
    26461 aatttgtaac agtttctgag ttgataaaat aatttttagg ggggagattt acagtatgat
    26521 tcttatgata gacaattatg attcttttgt atacaaccta gtccaatata tagaagaatt
    26581 aggggaagca gttgtagtaa aaagaaataa tgaaatcaaa ataagtgaca ttgaagaact
    26641 aaatccagag gtaattgtgt tatctccagg accgtgttct ccaaaagaag ctggtatttg
    26701 catagatatt gttgagcatt ttaaggggaa aaaacctata ctaggaattt gtttaggtca
    26761 ccaaactatt ggatatgttt ttggaggaga tattataaaa gcacaacagc ctgtgcatgg
    26821 aaaagtatat agtatcaacc atactaataa aggagttttt aggggactta aaaatccttt
    26881 aaatgtcact aggtatcatt cactaataat tgattccaat acagttccaa aagaattaga
    26941 aattacagca attactgata aaggggaaat aatggggatt aggcataaga aatatttaat
    27001 agaaggagta cagtttcatc cagaagctat tttgtcagag tatggtcatg aaatgcttga
    27061 aaattttata acagaagcta gagaaagggt acatatgtaa tatgataaga gagataaata
    27121 ctaaattaaa ttcttttgag atatttacta tttttagaaa tgaacatgac agttttatat
    27181 tagacagtgc tatggataaa gaaaaattgg gaagatattc ttttataagt agccaaccat
    27241 ttaaggtatt aaagtataag gatactgatg aaaatccttt agaagtttta aaagaagaac
    27301 tacataagta tagggttgta aatgatacta atcttccatt tgtaggaggt gctgtgggat
    27361 atttatctta tgatttaggt aattacatag aaaatttacc tagaactgct gtagatgata
    27421 ttgagatgcc tgatatgtat tttggatttt ataatcatgt aatagttata gaccatcttg
    27481 tgcaaaaaac ttatatagct actccaaaca tagatatcga attagaagaa aagataatag
    27541 atgatataga acaaagaata ttaaaagaag agaaaaaagg tatagatagc atatgttatg
    27601 aagaaaaaga agtaacacct ataagactta aatctaattt tacaaaggaa gaatttaaaa
    27661 atgcagttca aagtgttaga gagtacataa gacaaggaga tatatatcaa gctaatttaa
    27721 cacaaaggtt cagtggagaa acagaactta caagctttga attgtatagg gacttaagaa
    27781 gatttagccc agcaccattt ggggcattct taaactttga agatacacat atattatcaa
    27841 attctccaga aagatttata agatgtgtta ataagagaat agaaactaga ccaataaaag
    27901 gtactcgtcc aagagggaaa gataaagaag aagatttaag acttcaacaa gaacttagaa
    27961 atagtgaaaa agatagagct gaactactta tgatagtaga tttagaaaga aatgatattg
    28021 gcagaatatc taaaactgga agtgttaaag ttccagagct atttgtaatt gaaccatatg
    28081 caaatgtaaa tcacttagtt tccacagttg taggtgagtt aaaagatgat aaagatgcta
    28141 ctgatgtaat aaaagcaact tttccagggg ggtctataac aggagcacct aagattagag
    28201 caatggaaat tatagacgaa ttagagccaa cgcaaagaaa tgtatatact ggttcaatag
    28261 gttatatagg ttttaatggt gatatggatt ttaatatagc aattagaaca ataatcaaaa
    28321 atgacaaaaa agtctatttt caagttggag gaggaatgac ttgggattct gacccagatg
    28381 aagaatatca agaaacactt gataaagcta aatccataat gaaggctttg aggggatatt
    28441 atgaagaata atgtaggttt tgatagtagt ttaagtaaat ttggaattgg tttgtttgaa
    28501 actattagag ttaaaaatgg aatagcagta gatttagata tccatataga aagaatgcta
    28561 agttcaataa attgtttgga tttagatata aactataaaa aagatttctt gataaatgaa
    28621 attgttacat atataaaaaa agaaaatgtc attaataaag cacttagaat tactgttttt
    28681 gatgaaggtt ataatatatc tattagggat attccatata atcaagagac gtatgagaag
    28741 gggtttaaat tagctatatc gccaatagtt agggggaata gtttaattca tagacataag
    28801 actactaatt attttgaaaa catatatacc aaaaatatcg caaataagac tggttataat
    28861 gatggtatat ttttaaattc agaaggtgta atattagaat gttctatgag caatattttc
    28921 tttataaaag gagaaagagt gtacactcct agtgatagat taccgatatt aaatggaata
    28981 ataaaaaaaa ggattataga gatttgtgac gaattacata tagagttgat agaaaatgaa
    29041 attaatatat cagaaattag tagttttgat tttgtttttg tcacaaatag tcttatgggc
    29101 gctataaagg ttacagaaat tgacaaaata aaatttaata aagaaaatgt tgtttttgat
    29161 aaaatagtga agttattaga ataaattaaa atattaacaa tttgttggag acaaaagatg
    29221 ataggttgta tttatgaagt atacaatgaa gagatgacta aaataataga aaaccaaaac
    29281 actttggctg tatatttagt aggttctagt aaagacgtag attttactct ttatgatgta
    29341 gagataaacg atattgatgt gttcgttttt gtaaaagaag gggaaaaaca agagagaata
    29401 ctgtttaaaa aaaagggaat agaatttgat gtaaattact tttcaaaaga tggtgtaaaa
    29461 aaattattat caaataggga atatttcttt gtgaaagaaa tgaaagatgc taaagtactg
    29521 tttgataggg agaatatatc aaatattata aaagatataa gtagagatat ttacttagaa
    29581 ggacccagta aaatgtcatt agaggaaaag tcttttataa agcaagatat tggagcaaaa
    29641 atatcgaatt taaaaaataa agaaaaattt gatgtttttg aatatcattt tttgacgaat
    29701 ttatatttaa aagatataat aattgggtac tttaatataa atgataaatg ggtaccaaaa
    29761 gacaaaaaat tgctaaaagt tttaaaaaaa gaaaataatg agctttttga actagcgtca
    29821 aaagtaagtg aaaattatga ctatcaaaga cttttagatg tttataatta tatttttaaa
    29881 gaaattgaga caaaagaggt tataaaacta atcttctagt cacaaatcaa tggggggagt
    29941 ttaaatgaat aaagttgata aagaaaagat acaacatgca gtaagagaaa tactagaggc
    30001 aattggagaa gacccagata gagaaggtct aatcgagact ccaaatagag ttgctaggat
    30061 gtatgaagag atattctctg gtcttagtga agaaccaaga gaccatttaa aagttctttt
    30121 tgcagatgaa aaacatgaag aattagtatt ggtaaaagat attccatttt attcatgttg
    30181 tgagcatcat ctagttcctt tctttggaaa agctcatatt gcttatttac caaaaggagg
    30241 aagacttaca gggctttcta aacttgctag agtaatagat acattggcaa aaagaccaca
    30301 attacaagaa agaatcacta aaaatgctgc agatataata atggaagaat tacaacctta
    30361 tggtgtctta gtagtagtag aagctgaaca tatgtgtatg acaatgagag gtgtaaaaaa
    30421 accaggttca aaaactgtta catcagcagt gagaggaata tttgaaaagg acattgcgtc
    30481 tagagccgag gctatgagtt tgattactat gaagtagcaa tttattatag atataatcat
    30541 ttattgagat ataaaattta atttaaggga gtgtcattaa ttgtgtttga ttatggtaaa
    30601 agaacttata taatgggaat attaaatgta actccagata gtttttctga tggaggagat
    30661 tttaataact tggacatagc aatacaacat gcaaaagata tggttgacca aggtgcagat
    30721 ataatagact taggaggaga gtctacacgc ccaggtcata gctatgtaga ctcagacgaa
    30781 gagctaagaa gagtaatccc tgtaataaaa aaacttaaac aggaattgga gataccaata
    30841 tcaatagaca cttataaggc agatgttgca gaagaagcat taaaattagg tgtcactatg
    30901 gtaaatgatg tttggggtct tagaaaagat aaaaatatgg cttctgtaat aggaaaatat
    30961 gatgcagaag tgtgtataat gcataatcaa gatgggacaa actatgacaa agatataatg
    31021 gagtcaataa aagacttttt taaggtaagt atagaaatgg caatgagttg tggtgtaaaa
    31081 aaagaaaaaa tagtacttga cccaggcgta ggatttggca aggattttga acaaaatatt
    31141 gaagtattga gaagattaaa tgaattaaaa gatttagggt atcctatttt gcttgggaca
    31201 tccagaaaat cagtaatagg aaaagtactt ccagttgagc ctaaaaaaag attagaaggt
    31261 acaatagcta ctactgttct tggtataaga gatggcgtag atatagttag agtacatgat
    31321 gtttatgaaa atttaatggc agctagaatg actgatgcaa tatatagaaa gtaggtataa
    31381 tataatggat aaaatattac ttagtaatct aggattctat ggatatcatg gtgttcttaa
    31441 agaagaaaat tttctaggtc agaaattctt tgtcgacatg gaactttata ttgattcaag
    31501 agaagcaggt cttagtgacg atataaataa atctgtaagc tatgccgagg tttacaatgt
    31561 tgttaaagat attactgaaa ataaacaatt taatcttttg gaggctttag cggaaaatat
    31621 tgctgaagaa gtcttgaata aatttatact tataaatgga gttatggtca gagttagaaa
    31681 gcctgaggct cctgtaaatg gtatttatga ctattttggt gttgagataa ggagaacaag
    31741 agatgaataa ggcttatttg ggaataggta ctaacatggg tgatagattt gataatttat
    31801 caagagcatg tgaacttttg aaaaatagtg attctatata taaagtaaag gaatcaagtt
    31861 tatatgaaac aaaaccatgg ggatatacag aacaagcaga ttttctaaat atgtgtgtag
    31921 aaatagaaac agaatttgaa ccttatgagt tgttagaata ttgtcaagaa atagagagag
    31981 aactacacag agagagaata gttcattggg gaccaagaac tatagatgta gacatactat
    32041 tttttaatga tgtagcttcc actgatgaaa gactaacaat acctcatccg aggattcaag
    32101 atagagcttt cgttttgata ccacttatgg atttaaatga agaacttata ataaatgaaa
    32161 aaacagtaaa agaacattta aacctcctat ctgctgaaga aagagaagaa gtaaaggagc
    32221 ttgtaggtta tgaaagaaaa cctatttaat aataattcaa agatagagat aaatacaaat
    32281 aacaaaaaga taactgtaaa aaaagataaa cttataatag ctggaccatg tgcaatagag
    32341 agttatgagc aacttttgga gactgctaaa tttgtaaaaa gtcagggagc aaatatttta
    32401 agaggaggag cgtacaagcc aagaacatcg ccaaattcat ttcaaggatt aaaaaaagaa
    32461 ggtcttaaaa tattaaaagc agttaaagac gaagttggta tggctgttat tacagaactt
    32521 atggatgtaa gagatatcga cgaactgtat agtataagtg atataattca aattggttca
    32581 agaaatatgc aaaactttac tcttttaagt gaagtgggaa aacaaaataa acctgttatg
    32641 ttaaaaagag gaatagcatc cacaattact gaatggatag gtgcttctga atatatagca
    32701 atagaaggaa atagtaacat tataatgtgt gagagaggta taagaacgta caatgattat
    32761 actagaaata cattagattt agctgcggta cccattattc aaaaggaaac aggtcttcct
    32821 gttgtggtag acccaagtca tgccactgga gttagatatt tggtaaaacc tatgtcactg
    32881 gcatcttttg catgtggagc agatggaata atggtagaag tacatcctga cccagaaaat
    32941 gctttgtcag atggtgctca atctctttgt tttaatgaat ttgaagattt aatgaaatca
    33001 attaataatt attaaaagag gcaagagcct cttttttatt cgtaatcatt gctaaggtaa
    33061 atttataagt tataatttga tagttagatt atttaa
//
LOCUS       DAMJVX010000023        31682 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMJVX010000023 DAMJVX010000000
VERSION     DAMJVX010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.025"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            125..442
                     /gene="flgB"
                     /locus_tag="PC407_001070"
     CDS             125..442
                     /gene="flgB"
                     /locus_tag="PC407_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDF2496528.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYVTFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            450..854
                     /gene="flgC"
                     /locus_tag="PC407_001071"
     CDS             450..854
                     /gene="flgC"
                     /locus_tag="PC407_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDF2496529.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            864..1181
                     /gene="fliE"
                     /locus_tag="PC407_001072"
     CDS             864..1181
                     /gene="fliE"
                     /locus_tag="PC407_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDF2496530.1"
                     /translation="MPITQINNLMQGAKLDNLIGETKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1206..2756
                     /gene="fliF"
                     /locus_tag="PC407_001073"
     CDS             1206..2756
                     /gene="fliF"
                     /locus_tag="PC407_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDF2496531.1"
                     /translation="MIASFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDVQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAIPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDEDVDENNQPTNSLINVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSATNNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKAEAKQELEEIKKEGFKKNIIKAVIGVVAVVAIGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            2769..3839
                     /gene="fliG"
                     /locus_tag="PC407_001074"
     CDS             2769..3839
                     /gene="fliG"
                     /locus_tag="PC407_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDF2496532.1"
                     /translation="MSTEITNVNAFDLSDINESDKKEKIEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRDESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQDPALAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEAAQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            3832..4563
                     /locus_tag="PC407_001075"
     CDS             3832..4563
                     /locus_tag="PC407_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDF2496533.1"
                     /translation="MNNKIIKSKKASYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            4567..5886
                     /gene="fliI"
                     /locus_tag="PC407_001076"
     CDS             4567..5886
                     /gene="fliI"
                     /locus_tag="PC407_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HDF2496534.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGIAIG
                     EPPAQKGYTPSVFAILPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            5918..6364
                     /gene="fliJ"
                     /locus_tag="PC407_001077"
     CDS             5918..6364
                     /gene="fliJ"
                     /locus_tag="PC407_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HDF2496535.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIESNLE
                     NLESNYSKYSISKNNDSVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6392..7597
                     /locus_tag="PC407_001078"
     CDS             6392..7597
                     /locus_tag="PC407_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HDF2496536.1"
                     /translation="MKIQADSYNIIKDIGNRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRRETSKQNVSSDKRENSDNECHLDEDKSNHLEDENTYEKDCSNNNEALHQLLNLLK
                     DNYKKDKDINTILNNLKKLSISDELKQELKLNKNILNKIANQNLELNRASTNNPNDIE
                     NLDIMKLRELLENNASEDIEVISKTTENDTKSIDLYSFDSNRIDTLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            7615..8223
                     /locus_tag="PC407_001079"
     CDS             7615..8223
                     /locus_tag="PC407_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HDF2496537.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEEEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDANSGEENDSSEQESPEEV"
     gene            8250..9233
                     /locus_tag="PC407_001080"
     CDS             8250..9233
                     /locus_tag="PC407_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HDF2496538.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGVIYDGEYKADT
                     GEFIKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GNIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9246..9449
                     /locus_tag="PC407_001081"
     CDS             9246..9449
                     /locus_tag="PC407_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HDF2496539.1"
                     /translation="MIRVTDLNGRRYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKVNVGYIRSEK"
     gene            9449..10267
                     /locus_tag="PC407_001082"
     CDS             9449..10267
                     /locus_tag="PC407_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HDF2496540.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEVNEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10257..10952
                     /locus_tag="PC407_001083"
     CDS             10257..10952
                     /locus_tag="PC407_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2496541.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGISMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKADSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            10964..11407
                     /locus_tag="PC407_001084"
     CDS             10964..11407
                     /locus_tag="PC407_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HDF2496542.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11422..11796
                     /locus_tag="PC407_001085"
     CDS             11422..11796
                     /locus_tag="PC407_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliO/MopB family protein"
                     /protein_id="HDF2496543.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMNKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            11797..12462
                     /gene="fliP"
                     /locus_tag="PC407_001086"
     CDS             11797..12462
                     /gene="fliP"
                     /locus_tag="PC407_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDF2496544.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12475..12744
                     /gene="fliQ"
                     /locus_tag="PC407_001087"
     CDS             12475..12744
                     /gene="fliQ"
                     /locus_tag="PC407_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDF2496545.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            12756..14570
                     /locus_tag="PC407_001088"
     CDS             12756..14570
                     /locus_tag="PC407_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HDF2496546.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTARMIFFSMDGHHVLIRGIRYSFELIPIGKSFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPVSGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVLKR"
     gene            14579..16654
                     /gene="flhA"
                     /locus_tag="PC407_001089"
     CDS             14579..16654
                     /gene="flhA"
                     /locus_tag="PC407_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDF2496547.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVSKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAEIKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            16629..17645
                     /gene="flhF"
                     /locus_tag="PC407_001090"
     CDS             16629..17645
                     /gene="flhF"
                     /locus_tag="PC407_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HDF2496548.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKLIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVVNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            17642..18520
                     /locus_tag="PC407_001091"
     CDS             17642..18520
                     /locus_tag="PC407_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HDF2496549.1"
                     /translation="MIDQAQILRKMAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18534..19235
                     /locus_tag="PC407_001092"
     CDS             18534..19235
                     /locus_tag="PC407_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDF2496550.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELNVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19251..19673
                     /locus_tag="PC407_001093"
     CDS             19251..19673
                     /locus_tag="PC407_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF2496551.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEIRSNNHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELSLIRSCYKI"
     gene            19658..19843
                     /locus_tag="PC407_001094"
     CDS             19658..19843
                     /locus_tag="PC407_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF2496552.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            19884..20657
                     /gene="flgG"
                     /locus_tag="PC407_001095"
     CDS             19884..20657
                     /gene="flgG"
                     /locus_tag="PC407_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF2496553.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNDYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            20678..21439
                     /gene="flgG"
                     /locus_tag="PC407_001096"
     CDS             20678..21439
                     /gene="flgG"
                     /locus_tag="PC407_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF2496554.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRIYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNIDGLGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21462..22343
                     /locus_tag="PC407_001097"
     CDS             21462..22343
                     /locus_tag="PC407_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2496555.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMIVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVIKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTKKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22346..22720
                     /locus_tag="PC407_001098"
     CDS             22346..22720
                     /locus_tag="PC407_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2496556.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELNTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            22752..24155
                     /locus_tag="PC407_001099"
     CDS             22752..24155
                     /locus_tag="PC407_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2496557.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNVKVDDYKNEFLHKSSSNHKIND
                     YNNVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEADVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24271..26208
                     /gene="htpG"
                     /locus_tag="PC407_001100"
     CDS             24271..26208
                     /gene="htpG"
                     /locus_tag="PC407_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDF2496558.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITIQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            26596..27726
                     /locus_tag="PC407_001101"
     CDS             26596..27726
                     /locus_tag="PC407_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2496559.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDIDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            27953..28975
                     /gene="splB"
                     /locus_tag="PC407_001102"
     CDS             27953..28975
                     /gene="splB"
                     /locus_tag="PC407_001102"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HDF2496560.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            29157..30068
                     /locus_tag="PC407_001103"
     CDS             29157..30068
                     /locus_tag="PC407_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2496561.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYNTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            30083..30499
                     /locus_tag="PC407_001104"
     CDS             30083..30499
                     /locus_tag="PC407_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2496562.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(30593..30985)
                     /locus_tag="PC407_001105"
     CDS             complement(30593..30985)
                     /locus_tag="PC407_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2496563.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            31126..31521
                     /locus_tag="PC407_001106"
     CDS             31126..31521
                     /locus_tag="PC407_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2496564.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
BASE COUNT        12980 a         3366 c         5856 g         9480 t
ORIGIN      
        1 ttatattaat gtaatagaaa tgtaattaat ttggatgaat aaagaagtca ttttttgtaa
       61 tatagcaact tcatttttta ttaataagca aaataaataa aatatatttt gaggaggagt
      121 gagtgtgaat acatatgaca ttataaaaat gggattagat gcaactaatt taagaagtca
      181 gacaatagca aataatatag ccaatataaa tactgaagga tataagagac attatgtaac
      241 ttttgaagaa acattaaatg atgctatgcc aaaaatagaa gaaaaaacag acaattctaa
      301 aggattaaga gtggatggca ataatgtaga tatagaagaa gaaaaagtaa atcaagcaat
      361 ggcaacatta caatataatg ctttgataag ttttgcaaat ggaaaaatag caatgactaa
      421 atcaataata agtgggaggt aattaaaagg tgagtgtgtt cagtggaatg agaatatctg
      481 ctagtggatt aacagcagaa agcttgaaaa tggatatcat atcgtccaat gtagctaata
      541 taaatgccac aagaggtcca aatggagaag catataggag aaaagttgtt tcatttgaag
      601 aaaactatga taataaatta ggtatgctag gagtaaaagt tacatctgtt gaagatgata
      661 aatcacctct taaagaagta tatgacccat ctcatccaga tgcaaagcca aatggatatg
      721 tagaatatcc aaatgtaaat atattggtag aaatgacaga tttaataaca gcttcacgag
      781 catatgattc taacgtagat acattgaatg ctcagaagtc tatgatatca aaagctttgg
      841 aaataggaaa gtagggataa attatgccaa ttacacaaat taacaattta atgcaaggtg
      901 caaagttaga taatttgatt ggagaaacaa aaccattaac tcttaataat aaagaagaaa
      961 aaaagaactt tgaagatgta ataatagata ctatagataa agttaatttt caacaagtga
     1021 cagcagatga tatgaaagaa aaatttataa aaggcgaaga tgtttctatg catgaagtta
     1081 tgttaatagg tcaagaagca cagctatcta tgcaatattt aatagaagtt agaaataaga
     1141 ttcatgatgc atatcaagaa atgaataagg tacaaattta gccttttgaa caggagtaat
     1201 aatcaatgat agccagtttt aaaaataaga ttaaagagca agttgctaaa tctaggacaa
     1261 agataagttc tctaaaaaag ggaactaaaa tagcattagg tataggggtt attgctatat
     1321 gtttagcgct agtatttaca gcaatatctg cgagcaaaaa taagtatgca gtcttatttt
     1381 cagaactaga tgttcaagat gctgcagata tatcagcaga attagagtct aaaaagatag
     1441 atactaaggt aaagggaagt acgatttatg tacctagaac agaagttgat aagttaaggc
     1501 tggagctagc accaagtctt acaggtggtt ctaaaggatt tgcactaatg gatgatagtt
     1561 catcaatagg acttactgat gaagagtttc agattaagaa gcaaagaatg atacaaggtg
     1621 aaatagaaaa aacaataaag agttttccac aagtggaaga tactagagta catataacat
     1681 ttggagagtc atcagtattt gagaaggagg ctattccagg gagtgcagca gtttatttga
     1741 tgttaaaacc agggacgact ttggaaaaaa accaaataaa atctgttatg gcacttatat
     1801 caggaagtgt tacaaatata ccagagaaaa atgttcaagt cattgataac aaaatgaatt
     1861 tattatctga aggtatttta gaggatgaag atgttgatga aaataatcaa ccaacaaata
     1921 gtttgattaa tgttgataaa aataaggcat ctgaacaagc attgaataaa caattagaaa
     1981 agtctatttt agaaatgcta gaaccaatat ttggtaaagg aaaagtaaaa gccacagtaa
     2041 atgctgattt aagttttgat acagctgaaa aaactgagat aaaaattgac cctgataaag
     2101 ttgcaataaa ggagacaagg tcgaaaaatt catctaaatc tgctacaaat aatgttcaag
     2161 gtgtagatgc taacatgaat aaccaaggaa ataacaatgg tactaataca gaagatagtt
     2221 tagatgagag ttttgagtat gaaacaggta agattgaaac tcacactata gttgcgcctg
     2281 gagaacttaa tagattgaca gcttctgttg caatagatgg aaaaatagct aaaggtgtac
     2341 aagctgatgt agaggatatt gttaataatg ctataggtat ggatggagcg aggggagata
     2401 ctcttagcgt tgtttcaatg gtatttgacc cagaagggaa ggcagaagct aaacaagagc
     2461 tagaagagat taaaaaagaa ggatttaaaa agaacataat aaaagcagtt attggagtag
     2521 tggcagtggt agcaattgga gctattggat acttaatata taagaaaaga agtgaacgag
     2581 aagatatgga ttatgaagat gaagattata tttctaaatc tgattctgtt gataggaagg
     2641 ataatcctct attaggagat gacccagaac tgacattaga ggaagctgtt aaaatgtatg
     2701 cagaagaaaa gccagaccaa gtaacagaga taataaaaac ttggttaaat gagtaagggg
     2761 gatagaaagt gtccacggaa attacaaatg ttaatgcgtt tgacttaagt gatataaatg
     2821 aaagtgataa gaaagagaag atagaaggtg agggtttaaa aaaagctgct gtattattaa
     2881 tgacattagg accaaatgtg tctagtgata taataaaaag cttgcctgat aaacaagttc
     2941 aaaaaatagg tttagaaatt gcaaatattt cttcaatagc ttcaaaagaa agaagagaaa
     3001 tcttaaaaga gtttgtagag ataaataggg caaaagatta tgttatagaa ggtgggcttg
     3061 aatatgcaag agagttgtta agcaatgctt taggaaatgc taaagcaaac aaattacttg
     3121 aaggtatatc aataaataca agtacaaagc cttttaaaca tattataaaa ctagatgtaa
     3181 agaaaatatt agaaatactt agggatgaaa gtgcacaaac tatagctgtt ttacttgcac
     3241 atatggagcc agaaaatgga gcagaagttt tatcatactt agattttgat ttacaaaatg
     3301 aagtagctat gagaataagc tcaatgggtt caatatctcc agaagtaata aaggtaattg
     3361 aaagttcatt agaagataaa ttacactcaa taagtggtga tgattttaag gattatggag
     3421 gagtgtctac cttagttcaa atattgatta attctgatag aaaaacagaa aagaatattt
     3481 tgggaactat tgaagaacaa gacccggctt tagcagctca aattaaatct catatatttg
     3541 tctttgaaga tattgtcaag ttagatgata taagtataca aaaagtatta aaagaagtga
     3601 ctatgaaaga tttagtatat gccttaaagg gtgtggatga tgagatttca gatgctatat
     3661 atagaaatca atcttctaga gcagcagatg cacttaaaga agaaatggaa atggttggtg
     3721 tcataaaagt tacacaggtt gaggctgctc aacaaaagat agcaaatata gtaagaaaac
     3781 ttgaaagaga aggtgccata actattgtaa ggaattttgg tggtgacttt gttgaataat
     3841 aagattatta aaagtaaaaa agcatcatat aaaggagtgc tagatttaac cataatagat
     3901 aaagttagag tgggagaatc aaatgtaaat aaagaaaatg attgtagatt tagagatgag
     3961 gtaataaaag aagctataga atctgctgag ataaaaaaga atgagatatt agaagaagca
     4021 aaaatgaatg cacaaaatat agaaaaacaa gcatatgaaa aagggtatag tcaaggtcaa
     4081 aaaaatggat atgaagatgg atataaagag tcatatgatg aatataccca aaaagctaaa
     4141 gatgaagcag attttataaa aaatcaggca gacttaatac tattacaagc taatgagaag
     4201 atgactgagt acataaaaga aaaaaataaa gagattgtaa gacttgctat taatatggct
     4261 agttctgttc taaaaaagca tttcgaagat gaagaatcta tgagtgattt gttaaagcaa
     4321 attattcaag attatgaagg aagtagttca tttatagtaa gatgtaattc tttctataaa
     4381 gataatatag agaaagagtt tgaattattc aaagaggaaa catcactaaa atcaagtgta
     4441 tttgttattg cagatgatag tatagataaa ggcaatgcta tcatccaaaa ggaaaatgga
     4501 agaataatag ttggtatcga ttgtgctatg gaaaaactaa aagaagaatt gcttggtgaa
     4561 taaagtatgt ataaaactga tatatctata gattttgaca aaataaataa gaaaatagaa
     4621 aatttagaac tcactatatc agaaggtaga gtaaagaaga taataggact tactgtagaa
     4681 gttgaaggaa taaaagcttt tgttggggag ttatgtgtta tatacaatca agtcaataaa
     4741 ccagtgaatt gtgaagtagt gggttttaaa gataaagaag tcatattaat ggcgttggga
     4801 gagctaacat tgatagctcc tggttgtaaa gttatttcta agggaatacc tttaagtgtt
     4861 atgtgtagcg ataatttact aggtaaagta cttgatggtc ttggaaatcc tatagacaac
     4921 tcagatgctg ttttaggaga tagatacaac ttgaacaatg aacctcctga cccaatgaag
     4981 agaaaaaaga tacgaaatat aatggaaaca ggtgtaagag caatagatgc atttactact
     5041 tgtggtgaag gacagagaat agggatattt gcaggaagtg gagtaggtaa gagtacgact
     5101 ctaggtatga ttgctaggaa tgcaaaagca gatgtaaatg taattgccct tattggagaa
     5161 agaggaagag aggtactaga ttttattgat aaagatttag gtgaagaggg aatgaaaaaa
     5221 tctgttgttg tatgtgcaac atcagataaa gctcctttgg ttaggctaaa aggtgctctt
     5281 actgctactg caatagcaga gtattttaga gaccagggaa aaaaagtaat acttatgatg
     5341 gattcagtaa ctagatttgc tatggcacaa agagaagttg gtatagcaat aggagaacca
     5401 ccagctcaaa aaggatatac accatctgta tttgctattt taccaaaact tatggagaga
     5461 acagggacat cagataaagg ttctataact gcattttata cagtattagt tgatggagat
     5521 gactttaatg agccaatagc agatacaaca agagggatac ttgatggtca tatagtttta
     5581 tctagagact tggcaaataa gaatcattat ccttctatag atgtattgaa cagtttaagc
     5641 agacttatga atgagattgc ttcaaaagaa gacataaaaa tagcatcttt tgcgagagat
     5701 atgcttgctg agtatagaga agcagaagat ttgataaata taggtgcata tgcttcaggt
     5761 acaaacaaaa aaatagatga agcaatctac tatcatgagc atattattaa ttttttaaaa
     5821 caaggaataa atgaaaagag ttcttttaat gaaactataa gtagtttaag aagaatcttt
     5881 gaataaacaa tttaaaaaaa tatttgaata ggttgtgatg gaattggata aaaattttaa
     5941 gtttagatta cagaaagttt tagacttaaa aataaaagat gaagaagaga taaaaatgga
     6001 atttgctaag attcaacaaa aaaaaataga tatagagagc aatttagaaa acttagaaag
     6061 taattacagc aaatattcta taagtaaaaa taatgatagt gttcaaaatc aaaaaataac
     6121 tattaactat ttgttagctt taaacaactc tataatggat ttatcagaag agctggataa
     6181 gtcgacaaat gaacttgaaa aagctagaaa gcagcttata agcaaacaga tagagagaaa
     6241 gtcattagaa aaattaaaag aaaaaaaata tggacagtat tataaagaag aacagttaaa
     6301 agaacaaagc actaatgatg agtttgcaag catgagttat ttgagaaata gacaagttct
     6361 ataaaatagt aggaagtgag gatattattg tatgaagata caagcagatt cttataatat
     6421 tattaaagat attgggaata gaaaaagctc atctaagaat aaaaatataa aaaataataa
     6481 atttgaagat aatctatata aggcaacaga ccataaaaag gttaaagata gaagagaaac
     6541 aagtaagcaa aatgttagtt cagataaacg ggaaaatagt gataatgagt gtcatttaga
     6601 tgaagataaa agtaatcact tagaagatga aaatacatat gaaaaagatt gtagtaataa
     6661 taatgaagct ttacatcaat tactgaatct tttaaaagat aattacaaaa aagataaaga
     6721 tataaacact atattgaata atctaaaaaa actaagtata tcagatgaat taaaacaaga
     6781 acttaagctt aataaaaata tattaaataa gattgcaaat caaaatttag agttaaatag
     6841 agctagtaca aataacccta atgatataga aaatttagac ataatgaagt taagagaact
     6901 tttagaaaat aatgctagtg aagatattga agtaattagt aaaactacag aaaatgatac
     6961 taaaagcata gacttgtaca gttttgacag caatagaata gatactctta actcaaataa
     7021 acagaaagat gatagttctg atattttgga aaaactagca ggggtaaatg gaaataagag
     7081 tggaaatttt ggacaagtaa taaataaaag cgttgatatg aataaaatta aaaatgataa
     7141 tgttaattta aaacagatag aatcaaatgt aatggatgat tctataaaag ctattaagca
     7201 tatgaaaaca aatgatatac aagaattgac aattaaatta agaccaaaag aattaggaga
     7261 tatgaacata caattgttaa aagatggtga aagtatgaga gctgttgtga ctgttttcaa
     7321 caaagatgtc tttgactcta taaataaaaa catagcagat ttaaagcaac atcttgaact
     7381 tacaaatgta aatataaaag atgtatcagt gcaaatgcat agcgataata caaatacatc
     7441 tgataccttt gataaagcat ttgaacaaca gaatagacaa aataaccaag aaaacatgag
     7501 taagcaaaat acaaatgata agcgtaacaa tgtagtcata gaagatgaga ttaaagaaaa
     7561 tattattgat gatgatagag tagatttact agcttagtaa gggggaagta ttgcatgtac
     7621 ggaatagcaa cggctttggc aggaactcag gtaaactata ggtcaaataa tttaagccaa
     7681 cttgaaagaa atattaatga tattgccaat aaacaagtta gaagtaaagg aaatcagaca
     7741 gtaacagaca atgggactcc aattatagga caaggcgatg atgctgataa agatttgttt
     7801 atgaagattc ttgtagcaca gatgggtaat caagacccaa tgaatccaca agacccaaca
     7861 gaatatgtat cacaattagc acagtttgca actttagaaa agatgtcagc tatgaatgat
     7921 aagctagata tacttttagc cttaagcaat tccactctaa taaattctgc tttgagtatg
     7981 gcaacatcat taattggtaa agaagtagaa gtatctgggg aagaggaaga agatgaaaac
     8041 attgtaggag tagtaaaatc cacatatata aaagatgggg aagtacactt agaattagaa
     8101 gtagatggaa gtgacaaaac tgtaggtgtt aaatatgaaa aattacttaa aataggagag
     8161 gtagatgcaa attcaggaga agaaaatgac tcttcagaac aagaatctcc agaagaagta
     8221 taatattaaa tgagaaaaga ggtaaattta tgataaaagc aatgtattca ggtgtaagtg
     8281 gaatgaaagc aaatcaaaca aaactagatg taataggtaa caatgtagcc aacgctggaa
     8341 ctacttcttt taagaaaagt tcagctagaa taacagattc attttatcaa actgtactat
     8401 atgcaagtgc tccaacagcg gcattagggg gaacaaactt aggacaagta ggtgtaggta
     8461 gtaagatatc aagtataaat aaagatatga cacaaggaaa tgtacaacca actaataggc
     8521 catctgattt aatgatagat ggagatggat atcttccagt agtaagacaa ggaacagtta
     8581 tgtatacgag agatggttct tttaatttag atacaggatt taaagcagct gatggtgctt
     8641 tgaagaatat taaaggtgga agacttgtaa atggtgatgg atatatgtta caaggtgtga
     8701 tttatgatgg ggaatataaa gctgatactg gagaattcat taaaaagggt gatgctaaag
     8761 gaaaagagcc tattgtaata ccacttcaaa gtataagtcc taaggatgga acagttcaaa
     8821 atgtactaga gtataatata tcaaaggatg gaacagtaga atttttattg agtgatggtc
     8881 aaagaactca atttgtctat gatatacctg atggtgctaa ggtaggagat acacctgctg
     8941 gaatagataa attaaaaggt acaatacaaa aagtgcaaac atatgcattc caaaatccag
     9001 caggactaga tgcagaagga ggaaacttat tcaagccatc agcaaactca ggagatccac
     9061 aaatagcagg agctacagga aacataatac aaggggcaat agaagcttcc aatgtagata
     9121 tagcagaaga atttacagaa atgataatag caagtaggtc tttccaagca aacagtaaaa
     9181 taatatcgac atcagacgaa atacttcaag aaataataaa tcttaagaga tagtaggtac
     9241 atattatgat aagagtaacg gatttaaacg gaagaagata catattaaat tcagacctta
     9301 tagtgagaat tgactctgta ccagaaagta ggattctcct tacaacagga gaaaaaaatc
     9361 ttgtaaaaga gtcagtagat gagatagtag ataaagtaat aaagtataaa agaaaagtaa
     9421 atgtaggtta tattaggagt gaaaaataat gaaaaaaaca gattcactaa cattcattgg
     9481 actaatatta tctacagtat tagttttatt tggggcagta aaaggaagta gttcaggatt
     9541 gaaaagtttt ttcgattttg catctatatt aatcacagta tttgggtcat ttggagcttt
     9601 gatgattaca tttactattg atgatataaa acttataaaa gatgcgctac aatactcatt
     9661 caagacaatg aatatatcga aattagactt attaaatcaa tttaaaacac tttctaaaaa
     9721 agcaagaaga gaaggacttt tatctataga aagtgatgta atgaaaatac aagacccatt
     9781 tatgaaaaaa gggttagagt tatgtattga tggccttgaa gtaaatgaaa taaggtctat
     9841 actagaactc gaaatagatt ctgttgaaga aaccaataac agatattcta aactatttaa
     9901 aacttggggg actttcgcac ctgcatttgg tatgttggga actctaatag gtttaataca
     9961 aatgttatct gaccttacat ctccagattt aatagcatcg ggtatgggaa aagcattaat
    10021 tacgacattt tatggttctt tacttgctaa tatcgctttg aacccaattg catataatat
    10081 agatgagaag actgaaaaag agatttatgt aaaggaaatg atgctggaag gtataataag
    10141 catacaatct ggggagagca gcatagtagt tgaggaacgt ttagctacat acctttcaaa
    10201 caatgagaag ctagaaatta tgaaatccaa taaaaataca gaaagagcaa tgagtaatgg
    10261 cgcgtaaaaa aaataagaaa aaggatgaga taaatccaga tgcatggttg gctacttatg
    10321 cagatactat aacactgatt cttacatttt ttgtgttatt gtatgctaca tcagttgtag
    10381 atatggaaaa atttaaaaac atagcagatg cgttaaaagg tcagttttct gggataagta
    10441 tgttcagtac aggttcaagt acaagtgaga agtctccaat agagagtgtt ttagtggata
    10501 aaaatgataa gtcaaattta gaacaggatt taaaagataa agttaataat tctaacttac
    10561 aaaatagtgt aacagtcaaa gatgatagta gagggatttt actagaacta gatgattcaa
    10621 ttttatttga ctctgggatt tctgaattaa aagcagatag taaagccact cttaataaag
    10681 tatatgacat gataaagatg atgaaaaata agatagttat agaaggccat actgataatg
    10741 tacctgtaaa atcaagtaca catgaatcca attgggattt atcttctagt agagcagtaa
    10801 gtgttgtaag atactttgta gaagaaaagg gcatggaccc tagactattt tcggcaacag
    10861 gatgtggaga gtatcaacct ttggtttcaa atgaaacagc aaagggaagg gctgagaata
    10921 gaagagttaa tatacttata actattgact aggaggaaga ggtttgttca aaaataaaaa
    10981 aattattata attattttga tactgattat tttctcagca ggagcaggtt tttttatatt
    11041 aggagaaggt ggaaataaac aaattgacaa gattgcaggt ttgtttattc caacagggaa
    11101 aggcaaagat gaagtaaaga ccgaaaatct tgacttagaa gagatggtat taaagctagc
    11161 tgatgaaggt gtaagatatt taaaagtatc aataactgta tcttatgatt cttcatataa
    11221 agatatagaa aaaaatacgc ctttaattag ggataatatt ataaattgtt ttatggcaaa
    11281 aagggcagat gattttactg ctgataattt aaagaacata aaagaagaaa ttaaaaatac
    11341 attaaatata caactaggtg aaaatgtaat tcaaaatata tatttcacaa atatagtagt
    11401 gcaataaaag gatggtaaga catgatactt tatttactaa aaatattaat atgtatccca
    11461 ataattttaa tattgatagt cttatcttta aaaattagcc aaggaacttt attaaaaaaa
    11521 tataatgata aatatataaa aatactagat attgtaaata taagtaaaaa caatagcata
    11581 ataattttaa aaataggtga agaaggatgc attatggcat cttcttcctc tagcatgaat
    11641 aaaataaaag atttgaccaa agaagaaata ttaagaatag aaaacgacat taaagaaaga
    11701 aattttaaaa ttcaagattt taaaatggaa agaattaaaa agaaatatga tttcagtaga
    11761 tatattggaa aatttacaaa aaaggaagat aagtaaatgg aactattatc aaaattacct
    11821 ttggctgatg taccatatac aagttcaatg caattgtttt tatttctaac tgcattaatg
    11881 tttttaccat ttgtaatgtt tatgatgaca agctttgtca gaatagttat aagtttatca
    11941 tttttgaaat cagcattagg agcacagcaa gcaattccaa gccaaatttt ggtagggctt
    12001 gcaatagtat tgacaatatt tataatgaga ccagtattaa atgagataaa cgaaaaggca
    12061 ttacaaccat atatgaaaga agaagttact atggaagaag ctatgaagga agcagaggga
    12121 cctattaaag aatttttatt aaagcaaacc agacagacag atttagattt atttgtagaa
    12181 caggctggtt tgaaagaaaa gaaattaact cgtgaaaata taccactatc tgttgtagtt
    12241 ccagcatttg ctataagtga actaaaaact gcttttcaaa taggtttttt aatatatata
    12301 ccatttttga taatagacct tgttgtggca agtgttttga tgtctatggg tatgtttatg
    12361 ttgcctccag tcatgatatc attaccgttt aagttattat tatttgtaat ggtggatgga
    12421 tggaacttga tagtaaaaac attgatattg ggatttggat aggagaaaaa tagtatgagt
    12481 gaatttatga taatgagtgt tcttaaaaat ggcatgttta caggagctat gattgcagct
    12541 ccagtactca ttgtttctct tgtagttggg cttttaataa gtgtgtttca agctacaact
    12601 caaatccaag agcaaacttt gacttttgta ccaaaactaa tagcaattct tttagtagta
    12661 gctattggtg gaagctggat gttacatact cttattagct ttacttcgga gatattcaat
    12721 atgatgtctc ttataactaa ataagtagga tatatatgat ttttgtattt attagaatag
    12781 tggcattctt tggaacacta aggatactat ttccagctgg tacacctgct ttatttaagt
    12841 cgctctttgc aatcattata tcaatattga tttcaagtac tatgcaaatt gaatatgcaa
    12901 caaatataga caacattgta ttgtttacct tatatggtat aaatgagacc ataactggca
    12961 ttatgcttgg atatataacc aatctatgtt tttatagtat cagaatggca ggttctatga
    13021 tggatcaaca gatgggatta tcaatgatta atatgtttga cccaaattca atgactcaga
    13081 caactttaat tgataatctt atgaactgga cagcacgtat gatatttttt agcatggatg
    13141 gtcatcatgt tttaattaga ggcataaggt atagttttga gttaattcca atagggaaat
    13201 cattcgtaga taataatata gattatataa taaacatatt tgtgcagtgc tttttaactg
    13261 gattcaaaat agcaatacct attgtacttt gtctgcttat ggcagatttt atattagggc
    13321 ttatatcaag aagtgtacct caactaaatg taatgataat tggtatgcca ttaaaaatac
    13381 tggttggtat tgcgttattt atcatatcaa tacctttaat tgtcaatcaa ataagtcatt
    13441 tattaagtca aataccaaag atgtatgagg gaactttcgc actagctcct atgtttttta
    13501 tgggttcaac agataaaaca gaagaggcta ctccaaagaa aaagggagaa caaagaaaaa
    13561 aaggtaatat agcaaagagt aaagaattac ccgttgcaat gactctactt gcattcactt
    13621 tattagtacc tacattattt tcatatgtag tggatacact taaatcttct cttaattatt
    13681 ttttgagtct agatttttat atgaatataa attattcaag tatagagaaa ttagttatag
    13741 caggattaat ggattttttt aagatatttt taccaatagc tattccattc ttagttttag
    13801 gtatagtcgc aaacctattt caagtgggaa tactgtttac tggtgaaact ttaaaaccca
    13861 atttatcaaa attaaatcca gttagtggat ttaaaaacat gttttctatg agaagtttgt
    13921 caaccttaat aaaggataca gcgattatat ctattttggc ttacatagga tatacattct
    13981 tccaagataa ttatttagat atattaaagc taggtaacat atacctgcca actttaatgt
    14041 acacagttaa agatttagta tattcaatat taagtaagat ttgtgtagct atgatagtta
    14101 tagcagttgc agattatgtt tatcaaagat acagtcataa aaagcaactt agaatgacaa
    14161 aacaagaagt taaagatgaa tataaaaact cagaaggaga cccagaaata aaagctaaga
    14221 taaaacaaaa acaaagacaa atttcgtctc aaagaaccat gcaagcagta ccaagtgcta
    14281 ctgtaatagt aaccaatccc acccacttat ctatagcagt gagatatgaa aaagggaaag
    14341 accaagctcc agtagtggtg gcaaaaggtg cagattattt agcttttaaa ataagggaaa
    14401 ttgcaaaggg aaatgacata cctataatag aaaataaacc tattgctagg ttgttatata
    14461 agcaagtaga aatagaccaa gaaattccag aggacatgta tcaagctttt gcagagatat
    14521 tagtagcagt atataaaatc aaaaatagat acaaagtcct caaaaggtag gataagtaat
    14581 gaataagttt aatttaaagg aaaatatgga cgtaatagtg gcatttggtg ttgtagggat
    14641 tgttcttatg atgatagtgc cgttacctac atttatatta gatattttac tggcaattaa
    14701 cataagttta tctgtgctaa ttttactaat gacattattt acaacaaatg tacttgattt
    14761 atctatattt ccaacagtgt tacttgtgac aacactattt agattgggat taaatctttc
    14821 atcaactaga ttgattttaa cacagggata tgcaggtgaa gttatagagg catttggaag
    14881 ttttgttgta ggtggaaatt acgtagtagg aataattata ttcttaatta tattaattgt
    14941 tcaatttgtt gttataacca atggttctgg aagagtatct gaagtaacag ctagatttac
    15001 gttagatgcc atgccaggta agcagatgag tatagatgca gatttaaatg caggagttat
    15061 agatgacaaa actgctagaa aaagaagaaa agagcttcaa caagaagcag atttttatgg
    15121 ctctatggat ggagcaaata agtttgtaaa gggagatgcc atagcaggtc ttatagtaac
    15181 agcaataaac ataataggtg gaattgtaat aggagctgta atgctgaata tgactcttat
    15241 gggtgcagca cagacatatg taagacttac tataggggat ggtcttgtaa gccagatacc
    15301 tgctctatta atatctacat cagcgggtat aatcgtaaca agagcttcta ctgatgaaaa
    15361 ctttggtgga ttagtaagta aacagttaac ggcaatacca aaagcagtaa tgatgactgg
    15421 ttgtgttcta atcgtacttg gtatgatgcc tggattacct aaaccagcat ttttttcact
    15481 aggtataggt gctatagcag caggttactt tttaggaaga gaaaagaaag atactttaga
    15541 agacttggag tttgaaaaac ctgatatggt aaatccagat aaaaatgaag tagaagatgt
    15601 aacaaactta ataaatgtag aatcaataga agtagaaatt ggatatggtt taatatcttt
    15661 agctgatgaa tcagcaggag gagatttact tcagagaata gcatcaataa gaagacaatg
    15721 tgcaatagac atgggtatag tcgtgcaacc cataagaata agagataatt tacagttaaa
    15781 tccaaaccaa tacaatataa aaataaaggg aaatgtagtt ggtgggtatg agataatgcc
    15841 taatatggtt ttatgtatgg accctatgaa tcaaggattt agtatatttg gaataaagac
    15901 aattgagcct acttttggaa tagaggcact ttggatagaa agtgacaagg tagaagatgc
    15961 agagataaag ggttatacag ttgtagattc atccacaata cttataactc acttactaga
    16021 tataataaaa tcaaatgcac acgaattact gggaagacaa gaagttaaga ctattataga
    16081 tgcagcaaga gaaaattata gtgctgtagt agatgaattg ataccagatt taatgacact
    16141 tggtgaaatt caaaaagtac ttaaaaatct gttaagagaa aaagtaaata taaaggatag
    16201 ggttacaata cttgaaacgc ttgcagacca gtctagaaat acaaaagata tagagcttct
    16261 tactgaatat gtaagaatag ccttggctag aagtatttgt aataatttag ttgatgatga
    16321 taagacaatt gtagtggcta cattgtctcc agaaactgaa gagttggtat ctaataattt
    16381 acaaaggtct gtaaatggaa cttatccagc agttgaccca gaaaatacaa ataaaatatt
    16441 tgagagtata caagaagtaa tgaacaatgt atatttcaat cataatatac cagtaatagt
    16501 agtatcacca aagataagag ctccatttag aaaattagtt gaaattgtat atcctaattt
    16561 gactgtatta tcattaaatg aaatacctaa cgatattaaa atcaaagcag aaggtgtggt
    16621 gagcatatat gatgacgaaa aagtatacag ctaacacaat tcaggatgct atgaatctag
    16681 ccaaaatgga attgggtgac aatattacat taatagataa aaaagaagta agaaagtctg
    16741 gaatacaagg tatcttttca aagaaagata ttgaactaac tattggatgg gaaaagagag
    16801 aaaacgtaga acaaaaagat ttaaaacgag agatagaaca attaaagtta attattaaca
    16861 atatggggtt tgataataag aatgataatg atatagataa aatatgtaaa aatctactaa
    16921 gtttagaatt aaatgaagaa attgtagaat ctataagaat tgatttgcaa gaaatgaagt
    16981 tcaatggaat tgatacaagc aaaaatttag tagaaatttt aaagaaaaag attaaaatag
    17041 aaaatcaagc aataaatggt aaaattgctc tagtcggacc accaggagta ggtaaaacga
    17101 ctacaatagc aaagttagcc gcaaaattag tttttgaaga aaataaaaaa gtaggagtaa
    17161 taactataga tacatacagg attggtgctg tagaacagtt gaagatatat actgatataa
    17221 tgaatattcc ttttaaaggg gtaataagtc ccgatgaaat ggaattggca cttgatgaga
    17281 tgaaagattg tgatgttgta ttaatagata caacaggtag aggctataaa aactctatgc
    17341 aaatactaga aatcaaaaat cttatagata aagctgaaac tgataatata catttagttg
    17401 tgaattgtac aacaagagaa agtgatacaa aagcaattat agattcatat agaaatgtga
    17461 attttaaaag cttaataatt acaaagctag atgaaacaat aacatatggc tcaatattta
    17521 acataatgaa ttatgcacaa aaaccaatat catatattac aacaggacaa aatgttcctg
    17581 atgatattat aaaacctaat gaagataaga ttataaggtt actattagga gttgaaagta
    17641 tatgatagac caagctcaaa ttttgagaaa aatggcaatg gaaaaaagag gacttgatga
    17701 gtttatagta gagaatgaga atgtacctaa gataattaca atagcttctg gtaaaggcgg
    17761 tgttggaaag agtaatctcg ctacaaattt gtctatatgc ttgaccaaac ttaataaaaa
    17821 agttcttata ttagatgcag acataggaat gtctaatata gatataataa tgggggtaaa
    17881 tgtaaaaggg actataattg atgtaattaa tggcgaaaaa aatatagaag atataatttc
    17941 acagactaag tatggtgtaa atataatatc tggaggttct gcccttaatc atatagaaga
    18001 ctttacagaa gctcaaagaa ataagtttat acatagcata gaacagattc acgatgtgga
    18061 ttttataata attgatacag gagctggtat gagcaaaagc ctattgtcat ttatatactg
    18121 tagtactgaa tttttcttaa taacaactcc agaaccgaca tcattgactg acgcatatag
    18181 tttattaaaa gctataagta attttggcat aaagagaagt gcaaatataa taatcaatag
    18241 ggtaattgat ttagaagaag caaaatctac atacaaaaga ataaaaatgg ttgtggacaa
    18301 atttctaaac cttaatctag aattgtatgg atatataata gatgacaaaa aagtaagttt
    18361 atgtgtaaaa aaacagatac cattcatttt agaatatcct acaagtattg catcaaaatg
    18421 tattatagcc atagcaaaga gaatggtaag tcagtcaaga gaagatattg ttgataataa
    18481 aagttttcta agaaagatgt ttagtatatt taaaagctaa ggaggcgtag ttaatgaata
    18541 gagaagaatt aataaaagag aatatgcctc ttgtaaagag tatagcaaga aaatttttta
    18601 taccagggaa aggttttgaa tatcaagact tagtaaatag tggagtcatt ggattgattg
    18661 atgcaataaa taaatttgat gctgaaaagg gggcaaagtt ttctagttac tcttatataa
    18721 aaataaaatc tgctatatta gatgaaatta gaaatcaaag tccaatttct aaacataatc
    18781 taactaaagt aaataagtat aatagagtgg tagaaaagtt gcaatctaaa ttattaagag
    18841 agccaacttc atatgaaatt gcaaaagaat taaatgtttc agaaaaagaa cttcatgata
    18901 tcgaaagtaa tatagacatg ttaaatattg tatcattaaa ctatgtaatc tttgaagata
    18961 caaatgaaac tgtacaagat gtaataagtg atagagaaga ggaagctcca gaaaatataa
    19021 tagaagaaga agaaaagtta gagattctat ctaaagcaat tagtaattta aatgaaagag
    19081 aaaaattaat actttcactg tactattatg aagatttaaa tctaaaggag atagggaaag
    19141 tgctaggtgt ttcagaatca agagtttcac aactccacag aaaatcaata agaaacttaa
    19201 gaaacaagat aaaagaactt aaatattcta tatagatggt gattgataag atgatacaaa
    19261 ttatattaat agtagtgtct attttaataa tagtaactat agttataaat ttacataagg
    19321 aatcaaaaaa taaagataac agatactttg ataaaatatt tgagcaagaa tatgaagcaa
    19381 atgatagatt aataattgca gaaaaaagaa gattcttttt agacaaagta cttgctgaaa
    19441 taagaagtaa taatcatata aaagtttatg aaaattataa cctaataaag gctaatgaag
    19501 aaagaaagga aaacatattt atagaatcaa aaaaggaagt aagacaagaa aatgagttat
    19561 ctcttaaagt gaaacatctt ataagttcag gttatgatga tgtggaaata tgtaagatgc
    19621 tagatattgg aaagggggaa ctgtcattaa taaggagttg ttacaaaata taaggatatt
    19681 attatgtgat aaaaaagtat tcttaggaat aggtattggt atattaatat caactttatg
    19741 cataatgctt tttagtaata atgatatgag taaggcagag atagaaaaaa aggccagagc
    19801 gttaggcatg aaatatacaa gtgagatgaa agttttagaa tagatgatac tgtttagaca
    19861 tttacaaaaa aggaggtcat aatgtgataa gaggattata tacagcagtt tctgctatga
    19921 taactaatca acaaaagcaa aatgttgttg tcaataacct agctaatatg gatacaaatg
    19981 gatataaaag taaacaactt ttgactaaaa gttttgatga attaaagttg gagggttaca
    20041 ataactacgc aaatggaact aaacaaaagc agattatagg cgatataagt ccaggtattt
    20101 ctatggatga aactatcaca aattttaatc aaggacctat aaaaactact gataataaaa
    20161 tggatgtagc tattcaaggt aagggatttt ttctagttag tgatgcagct ggaaatcaat
    20221 tttacactag aaatgggaac ttcagaacag acaatcaagg tgaccttata acaagtgaag
    20281 ggtattatgt acttgggacg aatactgcta ctggtgctgt aggtcatata aatgtaggaa
    20341 atcaaaaatt tgaagtggca aaagataata caatatcact agatggaaat gctatgtata
    20401 aattcaatat tgtagatttt aatgactaca ataacttaaa aaaagaagga gataacttgt
    20461 attctggagg aggagctatg aaagtaaata atgcacttac aatacaaagt gcgatagagt
    20521 cttctaatgt agatatggta tctgaaatta ataatatgat gaccatatca agagaatatg
    20581 aggcaaatca aaagataatt caggctatgg attctaaatt agcaaaaata gcaagtgaga
    20641 ttggttcagt aagataggtg ataggaggtg aatttttatg tacacaatgt tttatactag
    20701 taaaacagct ttatcagcaa atcaaagtaa actagatata atatctaaca atatagcaaa
    20761 ttctgattca acaggatata aaaaaataaa tatggaatat tctgatttag tggatgaagt
    20821 actaaataga ccatcatatc ctacaaatgg caatgatata tcgactggaa caggagctaa
    20881 ggcaacagct cccatgagaa tttatgaaca aggtgcattg gcccctacag atagtaagag
    20941 taatttggcg atagatggag aaggattctt tagagtgata agaaaagatg gtacatatgc
    21001 atatacaaga aatggtggat ttaatataga tggtttaggt aagattgtag atgataatgg
    21061 aaatatatta gatgttcaat ttgatgcagg atataactat aataatacga atatagatag
    21121 cacaaactta acagtttcaa gaaatggcga gttattcgta gataataaaa aaataggaaa
    21181 aatcaattta tatcaaccta taggaactca aaattttata tctgaaggag atagtttatt
    21241 cgtcgcaact gatgcagcac agattaaaca agttgagaaa gtagatatag ttcaaggcta
    21301 tagagagagg tcaaatgttt ctttacaaga agaatttgtt gacttaatag ccactcaaag
    21361 agcatttcaa atgaactcaa agggaataaa gacagcagat gaaatgtggc aaatagctaa
    21421 taatttaaga gctaaataat tatgtaaggt gaggaatatt catgaatgat atgaaagagt
    21481 acgattttgg caaacctcag agttattcaa gagaaaattt aacttgttta aattttttat
    21541 tagctgaatt ttgtaaaaaa tataaaaact atataattta tgaacttaag tgtaattcta
    21601 gtatgattgt tgatagtata gaccaagtaa attatcagac ttttttagat agagtaactt
    21661 cttcatgtgt agtagtacaa aatgcaatgg aacctgttat gaaaaacttt tcttttagaa
    21721 tagatagaag tgtagctgat atgtggattg atataacttc tggaggaact ggagttataa
    21781 aggataatga tagagagctt acagaacttg ataaaaaagt gcttttacat cttatggatg
    21841 atttagtaag aaatatgcac ttatttgaag gttttgaaaa tatacaagta ttggatacac
    21901 atacacatat taatttacca caattatgtt ctccaactgc accagtatgc gttgtagatt
    21961 taaaagtgct aaatgataat gagtatattg gaagtgtttc tctttgtttt ccgtataatg
    22021 gtttagaggc tctattagaa agtatatctg ttatagaatt ttttgataat gatgtaggag
    22081 atgattcaga ggaatttacc aaaaaaatct ataatagtgt atctaatata gaattatcag
    22141 ttattgcaga gataggaaaa gtatcaatta atgttgaaga cttacttaaa ctagaagttg
    22201 gagatgtaat agtaacaaat aaaaaaataa atgattatat agatatattt gtagaaaatt
    22261 caaaatcata tacagcaaca ccaggattta tatcaagcaa aaaaggtgta aaaataaatg
    22321 atgctgttgg aaaagaggtg tagttatgga caatagtaat cttatttcag atgaagaagc
    22381 aaagatgtta ttaaatgata aagttgaaag tgaagttgat ggggatgatg agttagcctt
    22441 agatgtctct gaagttaatc gaaataatat acaaaggata cttgacctta agttagaatt
    22501 atctgtagtt atagggagaa ctaagatgac tctaagagat attttaaatt tacataaagg
    22561 ttctcttatt gaactgaata ctttagcaga ccaagatgtg gaaatactaa tagataataa
    22621 ggttttggct tatggaaaag tagtggtggt agatttaaat tttggtgtaa agataacaag
    22681 tatagtaagt gaagaagaca tggtaaaatc tcttgtttag agataacatc aattaatact
    22741 taaggcatga ggtggtactt attttaaact taaatgatat aaaaaaagat atactaaaac
    22801 tcaataaaaa agactcatgt tctctatata atgaatctat taataatata aataataatt
    22861 gtatatcaac tggattaaaa tttttaaaac aagcaagaga tttaaaccca gatgatgttg
    22921 atatattaaa tctcacaggt cttgtaaact tactaaaatg taattttgat gatgctgtag
    22981 aaagtttcta taagagttca ctttgtgaaa aaactgctct gtgtgaaaag tatataaaca
    23041 tgcttacatc tgaggagttt ttaattttct ttgaaaagta caatcaactt ataaggttaa
    23101 taaataaagg aatgtataaa gaagctatac aagggtttaa acttataact gaccaatact
    23161 gtgatttaat agaaccttat gagttgcttg ccttactgta tgacaaggaa gaagaatata
    23221 taaaatttga tgaatgttta gaagtattga agattattga taaggaaaat tatttattaa
    23281 atcaagaaaa taacacatta gatatgtata atagtgctaa tatacaaact aaaagtaatg
    23341 taaaagtaga tgattataag aatgagtttt tacataaaag tagtagtaac cataaaatta
    23401 atgattataa taatgtaact aaagaaaaaa gttcaagcaa aaaccagaaa agttcagatt
    23461 ctaataagaa aaaaagtatt aaaaaaaata agtacctata tatagccata atattaggaa
    23521 tattattagt aggacaaagt atatatagtt cttataagat gaataattta actgataaaa
    23581 tatctaaaaa taatgaaact aaaaatgaag agtcaataga ttctaacttg actaagaaag
    23641 aaaataatat agaagaaaat gaagatatga gtaaggcttc taatataaat aagagtaata
    23701 aaaacattaa taaagaggct gatgtaaaat ctaaaaaaaa tactgtagaa aatgaagatg
    23761 ataaagattc tgacttattt acagaggatg aattgatgtc taaggcgaat actttaaaat
    23821 cagaaaaaaa aactaaatct tctatacaac tatataagaa agttgcagat ataggtgaaa
    23881 ataaagggaa tacatctgaa gcaacttatc aagttgctat tctcagtgaa aagttaaaag
    23941 actataaaac tgctgaagaa tattataaaa tgtatgtaga aaattactca gaaaaagatg
    24001 cttattttga tgaatcttat tataatttag ggatgatgta ctataacaat ggtgatttaa
    24061 aaaattctaa attgacatta aagaagttag ttaataaggt accaaatagt atgtataata
    24121 attcaaaagt aaaagaaatt ttaaaagaag aatagttaaa ttagataaga aaaataaatt
    24181 attttatttt aaaataatat gattataata tattgacata ccattttaaa aaatttaaaa
    24241 tattaattat aaaatgagga gggataaaaa atggaatttg aaaaagggag tatttcaatt
    24301 catacagaaa atatttttcc tataattaaa aaatggttat actctgataa agacatattt
    24361 ataagagagc ttataagtaa tggatgtgat gcagtaagca aacacaaaag acttgtatca
    24421 ctaggagaaa tatcagaaaa taagtcatca gattataaaa ttacagtatc tgtaaataaa
    24481 ggagaaggta cacttaaatt tattgacaat ggaattggta tgactgaaga agagataaaa
    24541 aaatacataa atcaagttgc tttttcaggt gctgaagatt tctttaataa atataaggat
    24601 aagatggaag agtcaaatga cataataggt cattttggat taggatttta ttctgcattt
    24661 atggtgtcta aaaaggtaca aatagataca ctttcatata cagaaggagc gactcctgta
    24721 agatggatat ctgaaggtgg tacagaatac gaaatatctg aatcagatgc tagaaatgat
    24781 agaggaacta ctataacact atttattgat gatgatagta aagaattttt agatgaattt
    24841 actgtaagag gaataataaa taaatactgt tctttcttac cagtagaaat atatttagaa
    24901 gatgttgaaa gattagaaag agaagctaaa gaagcagaag aaaaagctaa aaaacaaaag
    24961 gaagatggaa aagaagagat tgtagatgca aaagtaattg agcctttaaa cgacactaat
    25021 cctctatggt taaaatctcc aaaagactgt actgatgagg agtataaaga gttttataga
    25081 aaggtattca atgtatttga tgaaccttta ttttggatac atttaaatgt agattatcct
    25141 ttcaatttaa agggtatact ttacttccct aaattaaaaa atgagtttga actgactgaa
    25201 ggaaaagtaa aattatacaa caatcaagtg tttgtggctg acaatataaa agaagttata
    25261 ccagaatttt tacttttatt aaaaggtgtt atagactgcc cagacttacc attaaatgta
    25321 tcaagaagtt tcttacaaaa tgacagggat gtaagcaaaa tttctaaaca tataattaaa
    25381 aaagtagctg ataagttaaa atctctttgt aaaaatgaaa gagaagaata caataagttt
    25441 tgggatgata tacaaatatt tattaaatat ggttgcttaa aagatgaaag tttctatgag
    25501 aaggtaaaag aatgtatatt attcaaaact attgatgatg agtatataac aatacaagat
    25561 tatttagaaa aatgtaagga caaacatgaa aataaggtat tttatgtaag tgataaggaa
    25621 cagcaatctc aatacattaa actatttaaa gaatacgact taagtgctgt tgttttaaac
    25681 tcatcaatag atactcattt tatttcattt atggaatata aagagaatgg agttaagttt
    25741 aatagaatag atgcagacct ttctgatgta ttaaaagata aaaatgaaaa taaagatagt
    25801 gaagaaaaca aagaagagat agctaagata gaaggtttgt ttaaagaagc tgttggagaa
    25861 agagtgaaga attattctgt agaaggattg aaaaatgaag acactccagc tatggtatta
    25921 gtatcagaac aatctattag aatggcagaa atgcagtcaa gatttgctgg aatggattta
    25981 ggaatgaatt ttgaggaaga aaaaactctt gtaataaatg agaacagtcc aataatcaaa
    26041 aaattggttt ctcttaaaga tgatgaggaa aagaaagata agattacgct tatttgtaac
    26101 cagatagcag atttagcttt actttctaat aaagaattaa agccagatga gttagattca
    26161 tttgtacaaa gaagtaataa gcttatgagt cttttgatag aattataaga ttgaatttga
    26221 atacattgaa gcaaatttat aaaaagtatt caatatcagt gatgagaaag aagtgtctca
    26281 aaatgaactt ttcagttcat gaggcgcttt ttttatgtga aattaaattt attttaatca
    26341 tttcaacaat gaaaaatata tttatctcta ttttgagaca gaccgttctt tattttttta
    26401 tctttggtta atttttaaat atttaagtag gttctaataa aaaactctat ttagttaaat
    26461 atagttaact tgatatataa taagataata catataatta agtaaaagat tgttttttag
    26521 tttatgttta aatacataat tacactttgt taaaaacata aaattattac taaagtagaa
    26581 ataggaggtt ttagcatgaa ttttaattat aatttgccag taaatatctt atttgggaga
    26641 ggcagaatta atgatgtggg aaaagaagta tctaaatatg gcaaaaaagt attgatagta
    26701 acaggtaaaa atagtacaaa gaaaactgga ttacttgata aaacaataga tttactaaaa
    26761 gactctaaaa tagaatatga agtttttgat agagtagaac aaaatcctct tataactact
    26821 atatattcag gcgtagaaat tataaaatca actggatgtg attgtgtttt agggcttggt
    26881 ggtggaagca ttatggatgc tgccaagagt atagcttttt cttataaaaa cccaggagat
    26941 ttaaatgatt atatatttgg aattagacaa ggtgaagaag ctttacctat aatcttagtt
    27001 ccaacaacat caggtacagg tagtgaagga aattgttttg cagtacttac aaattcagaa
    27061 actaaagata aaaaatctat aaagaagaat agtatgtatg caaaagcatc aataattgac
    27121 ccagaactta tggttacaat gccaaagcat ataattgcat cagttggatt tgatgcactt
    27181 gcccataata tggaagcata tctttctaat ggaagaaacc ctcttgcaga tgtacaagct
    27241 atttatggga ttgaacttat atcagaaaac ctaataaagg tatataatga tgttaatgat
    27301 ttagaagcat gggaaaaaat aacacttgca agtactattg gaggaatgac gattggtaca
    27361 gcgggtacag ccttgccaca tggaatggag catcctgtaa gtggtcttag agatgtaatg
    27421 catggaaaag gacttgcagc gttgactcct actattatag aaagttcatg ggatattgat
    27481 attgaaagat ttggtaatat ttcaaaggta ttaggaggaa cttgtgcaga agattgttca
    27541 gatgcaatta gaaatttcct taaaaaaata gatttagata taaaactaag tgatttaggt
    27601 attgaaaaat cagatataga ttggttaact gataattgta tgaaaatatc agttgcaaat
    27661 attaagagac atccaaagta ttttaataag gaacaaataa aggaaatgta ccataagtct
    27721 ttataatttg actttacata tttagtttta tataaaaatt ataaattaat aataaaaaat
    27781 tataagtaat taatagtaaa aaaattgtgt atagaagtta tatatataat aaacttgtct
    27841 taagctttta ttttactttc taataaataa aggagtttaa aaaagggaag attaattaag
    27901 taatatttta taaaattctt cgatttaaaa actttaagta gaggtgaaat aaatgtttaa
    27961 tcccaaaaga ataatattcg aaaaaggaac tttagacact gatgtgggca aaaaaatata
    28021 caataaattt agagataata aagatgtaga gataattaat atatcatcaa ataggattaa
    28081 agaacacatt ccaggtgaaa atctatatga acaatataga agtgggaagc agactatggt
    28141 agttgggatt agaaaaagtc tgaagtttca aacatgtaaa ccttctgcta attatcaact
    28201 accacttgtc agtggatgta tgggaagatg tgagtattgt tatttgaaca cacaattagg
    28261 tgataaacca tttataaggg tgtttgtaaa tgtagatgaa atacttgaac agacaaaaaa
    28321 atatattgaa gatagaaagc cacaggtaac catatttgaa ggggcagcta catcagaccc
    28381 agttccagta gagccatata ctaattcttt gaagaaaaca atagagtttt ttggaagaga
    28441 ggaaaaagga agatttagat ttgttactaa atacagtgat gtggatagct tattggatgc
    28501 tgaacataat aatcatacag aaatacgatt tagtatcaat actcctagaa ttataaatga
    28561 acatgagcat tatacagcct ctgcaaataa aagaatagag tcagctgtaa aacttgctag
    28621 ggctggttat cagattggtt tcataatagc acctgtgttt ttatatgaag gttggaaaga
    28681 agagtataaa aatctgatta taagtataaa agaagcttta ccaaaggatt ttgataaaaa
    28741 aattatattt gaagttatat ctcatagata tacaactaaa gcaaaaaata gaatactaga
    28801 gatttatcca gatacactat tgccaatgga aaatgaggat agaaagttta aatacggtca
    28861 atttggatat ggtaagtacg tgtatgataa agaacaatta gatgatatga aagagttctt
    28921 tcttaaggaa ataaatggag tatttaaaaa tagtttaata aaatatataa tatagcaaat
    28981 cttacctaaa gatttgctat attttatttt tatatatttt atgtttataa taaagcaatg
    29041 agatttagca ctatcatata agaaaattaa aaagggcact atctatcaag aacatttttt
    29101 ctagttttga tactataaag ggtaagagta acaaaaatat caggaggagt tatagtatgc
    29161 atgcatggga gtctattcaa attacattgg atttaataga agcaaattta tcagaagaaa
    29221 tatcaattaa tgaattagca aataaagcaa atttatcacc attttactat caaagactat
    29281 ttaaaagatt ggtaaataag actgtaatgg agtatataaa gcttcgtcgt cttgcaagag
    29341 cttctgaata cttagcagaa cataagaatc gtatacttga tgtagcttta aattttggat
    29401 ttgctagtca tgagacattt acacgttcct ttaaaaaagc atatggatta actccagaaa
    29461 aatatagagc aaatccagta aaactcaatc actttattaa gccagaatta attttaaatt
    29521 atgctatggt tgatgaaggt gttccactga ttgcagatga tatagtgatt gaagtcaatc
    29581 gtaagacatt gagtaatcca aggacatttg ttggaataga aatagaagta cctatttgcc
    29641 agttggtggg tggtgaaaca actggaattg caatagtaga ggaattatgg atgaaacttg
    29701 gaagtcaaag acataatata ccaaatcaaa ttccaggtgg aaatgaattt gctgctcttt
    29761 atatgggaaa tgcaaaagaa ggaaattgta tgtatatggc aggtgtagag gtagagtcag
    29821 gtacatcagt agaaggatat aatacttttg agttgccagc taaagaatat ttagtatgtg
    29881 gatttgaggc agaaaacttt aatgagttag ttaattcagc tgtttttaaa gcagataaat
    29941 ttatggaaag atggatgaag aaacataatt taacaactac tgattttgca attgagatgt
    30001 attaccccac tacacctgaa gcagcatatc tggaacattg gattgttccg gttcctattg
    30061 aacaatagaa aggatgatga atatgaattg gcaagatgcg ttttttcaag atactaaacc
    30121 tacatttgag caaattaatg aatttattaa taatccatta tgggacaagt taaataaaac
    30181 tttggtttct acttataatg tagaacctaa gcttgaatac agtaaatgtt caatgcaaag
    30241 aggttggaat gtaaaatata aaaaaagagg taaatcacta tgtactttat atccacaaga
    30301 aggttatttt aaagctttgg ttattgtaag tgaaagcaat agagtagagg ttgatttatt
    30361 tattaatact tgttgtgatt atatcaaaaa aatatataat gaggtaaact tttttaatgg
    30421 aagtaaatgg ctaatgattc aagtagatag tttattggta ttaaacgata tgcttgaact
    30481 aattaaattt agagcttaac atatagaaca ggatgaatat ataagtcatc ctgtttttat
    30541 attcaatcag ataaattaat atatagtcta taatatgaat aatttagtaa atttacttta
    30601 tctttctaca ttctttgcaa ctaagtccac atgtgttttt ttcttgcata ttagtagcac
    30661 cccaattagc aaattgttgt aaaataggca caatactttt acctttactt gttaatgtat
    30721 actcaacttt tggaggaatt tcattatatt gtttacgatg aacaatacca gtagactcta
    30781 aatcttttaa tgattgagct agcatagtat ttgtaatgcc atccaatttt ctttttatgc
    30841 tactatatct aattggattt tcaactgcta acatacatat aataggtaat ttccacttac
    30901 caccgacaat gtctaatgca taacggattg gacaggttaa ttcttcaacg tttttagtta
    30961 tatttttttc agtatttata ctcattttaa ttactcctta gttatagtag tatctttttt
    31021 atactttatc agaaaaaact gcatacttgt tttttctgta ttttatatta aaattatatc
    31081 aaaggataat ccttaataca agtatttaaa aggggtgtaa gaattatgaa agcattagaa
    31141 tttttgaaca aggcaggagt ttattattta gcaacagttg atgagaacaa tcaagcacat
    31201 gtgcgtcctt taggatttgt tatggaatac aatggtaaac ttacattttg cacatctaat
    31261 caaaaggata tgtttaagca acttattttt aatccaaagg ttgaactttg ttgtatagac
    31321 caaaacctta atactctacg tattttaggt gaagcagtat ttgttacatc aaaagaaaca
    31381 caagctaagg cacttgaaat aatgccaagt ttaggtcaga tgtactctgt gggagatggt
    31441 aagtttgaaa tattctctat tgataaggca aaagttagtt gttgttcaat gagtggtaaa
    31501 aagattgatg tagagcttta attataattg aatcgacatc catagtgaaa attaagaaaa
    31561 cgttattact gaaatattga tagatttaat ttaagtgcaa taaaaagatt tttaaataat
    31621 gaatatgtaa aaatttttag ttattagatg tgtatttcaa taatgatata ctattttgaa
    31681 ta
//
LOCUS       DAMJVX010000024        31202 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMJVX010000024 DAMJVX010000000
VERSION     DAMJVX010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31202
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.026"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            152..1078
                     /locus_tag="PC407_001107"
     CDS             152..1078
                     /locus_tag="PC407_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2496565.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSENS
                     NAEIVTKNSDIFKNIEKYIDATINNNQKTMKYKHRDELFKNSNTNTYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENLDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            complement(1266..1886)
                     /locus_tag="PC407_001108"
     CDS             complement(1266..1886)
                     /locus_tag="PC407_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2496566.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYEEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTNMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(1858..3441)
                     /locus_tag="PC407_001109"
     CDS             complement(1858..3441)
                     /locus_tag="PC407_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2496567.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFL
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPEVQDIIYETLNNLNTCIRNQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNINDNGVKNTSFKTPLDITKITFDIIKHKGIISIKEQVENLGGTISISDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            complement(3585..5612)
                     /locus_tag="PC407_001110"
     CDS             complement(3585..5612)
                     /locus_tag="PC407_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2496568.1"
                     /translation="MNIFYMAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFITGLVLGALSHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAISNGKFLYKTSLVKLIRHEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQKSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLINGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTSILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYDNIQIKKIIKKQVLPFFNIPFLLGILDCIFAT
                     LVYKTALMQNLLGNSFSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKN"
     gene            complement(5625..6374)
                     /locus_tag="PC407_001111"
     CDS             complement(5625..6374)
                     /locus_tag="PC407_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2496569.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFDIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYAASFANRILIFKDG
                     HIIKELNKQPNNRHTFYELIIQEIAKLDTKR"
     gene            complement(6671..8323)
                     /locus_tag="PC407_001112"
     CDS             complement(6671..8323)
                     /locus_tag="PC407_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HDF2496570.1"
                     /translation="MNNKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYKMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSDILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKIEYEITINAITGKILEFEIDD"
     gene            8922..10043
                     /locus_tag="PC407_001113"
     CDS             8922..10043
                     /locus_tag="PC407_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2496571.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            10079..10492
                     /locus_tag="PC407_001114"
     CDS             10079..10492
                     /locus_tag="PC407_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2496572.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            10485..11024
                     /locus_tag="PC407_001115"
     CDS             10485..11024
                     /locus_tag="PC407_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2496573.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEASCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVAAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(11224..11709)
                     /locus_tag="PC407_001116"
     CDS             complement(11224..11709)
                     /locus_tag="PC407_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496574.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFDLDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            12560..12937
                     /locus_tag="PC407_001117"
     CDS             12560..12937
                     /locus_tag="PC407_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2496575.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            13345..15150
                     /locus_tag="PC407_001118"
     CDS             13345..15150
                     /locus_tag="PC407_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2496576.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIYKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNVCKNKDINIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            15227..15766
                     /locus_tag="PC407_001119"
     CDS             15227..15766
                     /locus_tag="PC407_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HDF2496577.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
     gene            16389..17603
                     /gene="dpaL"
                     /locus_tag="PC407_001120"
     CDS             16389..17603
                     /gene="dpaL"
                     /locus_tag="PC407_001120"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2496578.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLRDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            17936..19123
                     /locus_tag="PC407_001121"
     CDS             17936..19123
                     /locus_tag="PC407_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDF2496579.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYNGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSV
                     YKK"
     gene            19139..20722
                     /locus_tag="PC407_001122"
     CDS             19139..20722
                     /locus_tag="PC407_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF2496580.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            20750..22078
                     /gene="ssnA"
                     /locus_tag="PC407_001123"
     CDS             20750..22078
                     /gene="ssnA"
                     /locus_tag="PC407_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDF2496581.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            22277..23629
                     /locus_tag="PC407_001124"
     CDS             22277..23629
                     /locus_tag="PC407_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2496582.1"
                     /translation="MSQDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            23821..24930
                     /locus_tag="PC407_001125"
     CDS             23821..24930
                     /locus_tag="PC407_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2496583.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            25213..26604
                     /gene="hydA"
                     /locus_tag="PC407_001126"
     CDS             25213..26604
                     /gene="hydA"
                     /locus_tag="PC407_001126"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2496584.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            26598..29159
                     /gene="xdh"
                     /locus_tag="PC407_001127"
     CDS             26598..29159
                     /gene="xdh"
                     /locus_tag="PC407_001127"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HDF2496585.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            29311..30657
                     /gene="rpoN"
                     /locus_tag="PC407_001128"
     CDS             29311..30657
                     /gene="rpoN"
                     /locus_tag="PC407_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDF2496586.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
BASE COUNT        12123 a         3708 c         5444 g         9927 t
ORIGIN      
        1 agttaaatct acactattaa cttattatac ttaaaacttt aaaatgaaaa tatcaaagaa
       61 tactttttaa taaaaataat catttaataa gttatttaaa aatctaaaaa ctttacaaca
      121 aattatctat ttaaattgga ggagtgggaa tatgagtaat agtttttatc aattaaattc
      181 agcagtatta tattttaatg aaaaaagaga tgtaaataga ataaggttta atcactatgt
      241 aatgagaaaa gaaaatatac aaagattaaa aggaactctg ccaagaggac aatttatgat
      301 gacagtaagg aaagctgcat ctgaccttga tgtatctata tctacaataa gtagacttgt
      361 aaatgaattt atagatttag gtatattgag attaataagt aggggagtaa agggaaattg
      421 ttgttctgta tatagttatg tttgttctga aaatagtaat gcagaaattg taacaaagaa
      481 tagtgatatt ttcaaaaata tagagaaata cattgatgca actattaaca ataaccaaaa
      541 aacaatgaaa tataaacata gagatgagtt gttcaagaac agtaatacca acacatacag
      601 ccgtataaat caagagagta tagacaaaga ttatgatttg ataattcata ctgggtatga
      661 aacagaaaat gagacaaaga aaaaagaatt attaaaaaat aatttaaata aaaagaattt
      721 aaataaaaat acctttgaaa atttggatga agatttgact gaagattcta cttataaaaa
      781 tccagaaagc atacatgaaa ttattataag aaaattaaat agtgaatcag gaaaaaattt
      841 taaagctgat ttgagtataa ctaaggcact tatagataaa agattaaaag aagggtatag
      901 tttagaagat ttttacaagg ttatagaggc aaaggtaaga agatggagat atacagatat
      961 ggaaatatat atagaaccaa caattctgtt tgggtatgaa tttgagtcgt atttaaatga
     1021 ggagagttct ttatctgctt gtaattatgg tgatatgtgt tatgaagata tgtattgaga
     1081 tattagttgc tttttagttg tttaaaatat gtttatatat aaatataaat agtgtattaa
     1141 aatggagttg tctcaaaata gagagaagcc tcttcttaat tgtttaaatg atgaaaatat
     1201 atttgattta atacaaagga gagtgcctta tgagctagaa agttcatttt gagacactca
     1261 cttttttaat atactggaga aatataaccc attttaatgg cttttgtaat agcctcaact
     1321 gatgatgaaa catctagctt gtcatatatg tgttgtaaat gatttgataa tgttctttca
     1381 cttatataca attcatcagc aattattttt ctttttttac cactacagat aagctgtaaa
     1441 attcttttct ccatattagt taaatcttca ataattactt cagtattttc agatggaaaa
     1501 caggttaccc cttgatagac ttttaataat gaatcaatta aactatcagg gctaatattc
     1561 ttatttagga atccatagat acctattttt ttagcctcat gtttataaac tgggaggtcg
     1621 tatccagtca agattattat tttaatagtt gaattagtct ctaataaatt ataagctact
     1681 tttaagccat cacattcact taagcctcta aggtttatat ccattaagac aatgtctatt
     1741 tttttatctt gaatcatttc tgaaagatta tggatgtcct gtgtggaata aaactgttcg
     1801 atttcttcgt attcttctag tgcaattgac aagcttttag caaaaagcat atggtcatca
     1861 actaacaaaa tatttataag aaccatctcc tttcataggg attgtaatct gtacacaaac
     1921 tccatgaggt atattatcag aaatacttat cgttcctcct agattttcta cttgttcttt
     1981 aattgatata atccctttgt gttttattat atcaaaagta attttagtta tatctaaagg
     2041 tgttttaaaa gatgtatttt tcacaccatt atcattaata tttaatttta ttatattttt
     2101 ttctagtgct aaagctatcc atgcatggct accatttgaa tgtttataca cgtttgtcaa
     2161 taattcttta atcagtcgat atatcaatat atcatatggt tcaactaaga ataaagtatc
     2221 agaacactta aatgaaacac aaatgttttt ttgtggaaag gatgaagaaa cactttcaat
     2281 taaattctga taattttctt ttactgttaa attttttaaa ataacaggat gatagtcttg
     2341 catttgattt cttatacatg tatttagatt gttaagtgtt tcatatataa tatcttgaac
     2401 ttctgggcga tatgacttac tcatcatgtt ttttattgaa agcaggttct gtaatatgtc
     2461 atcatgtaaa aaattagaaa actctgtttt tagttgttct tcttgttgta atttttctaa
     2521 tgcaaaagta tacttgctat ttttaactac tttatttttt tcatctctaa ggttaaactc
     2581 caaaataatg ttacagagga aaataagcat aaataaaaca tttatagcta ttaatattat
     2641 agaaaaattc ccattggcaa atagtacgac caagctaatt actaaaatac ttataaaaag
     2701 aaatatagcc ctttgtttta tgttaaaaac tgttgaaata ggaaaactat cattttcttt
     2761 aagtgcaata ccatgaatac tcataaaaaa taccaataca gttagataga ttccaaaatt
     2821 accaatatga tttgatattc ttagtgtaaa aaaataataa gtaaggaatc ctataattgt
     2881 tataaatact gaaatcgata taggcttttt ttcgcttttt atgacaaaaa caaccctttt
     2941 caaatgataa aaaataatat ataaaaagct tattatacta acaacaaact gtattccgta
     3001 catacaagca tacgttctgt tagaaataaa tgctcctaca attgcaccaa tgcaggtgat
     3061 aagcaataaa atatctgtta tcttacggaa attgtatcca ctaccttgaa acaaaaataa
     3121 aaatataaat ttaatagaga catacactat aactgtgctt aatattttaa aaataatagt
     3181 gcctattggt gtggttacaa atgataagag taaataccaa ctgtctagtg ctagtaatcc
     3241 acaaaataat gaaataatgt tattttctcg gacattacaa gaggttatat atgtgatgtc
     3301 tatgagtaat aatgttgaaa ttattaaggg aataatgctt tggatattca tataagtttc
     3361 agtgcttgta gatattccca tgaagagaag tgatagaatg tagtttccta aaaatagtat
     3421 ttttaaatta aaatttgtca atcttataca cctccttatg agttaattat attaatttta
     3481 tgactaaatc aaacctaaga atctataatt ttaaattata tcaaatactt ataaatgaag
     3541 tataccaaat cgtcacacct ctaagcttga cgatttggta aatattaatt ttttaataca
     3601 attttacaac aggatttaat agtaagtata taatatatca aatacactac agaagttaag
     3661 aaaattgcaa taatcacagg tatgtattgt gaaaatgaat ttccaagtaa attctgcatc
     3721 aaagcagtct tataaaccaa ggtagcaaat atacagtcca atataccaag taaaaacgga
     3781 atattaaaaa atggcaatac ttgtttttta ataatttttt taatttgtat attatcataa
     3841 cccattttct gtaaaatctt ataatcagat gttgaatctg aaattgctga tatattgtta
     3901 aagtataaaa tacttccaac agcaataaag aataatacca caagaaatcc gattaacaga
     3961 aatgtagaac tattaattga taataaatca tttatcctgt gagaatatcc ttgtaaataa
     4021 gggcttccat ctaaataatc tgaaatatta ctatataaat tttcattatt tttaattgat
     4081 ttaccattaa tacttaaaat gcttgtcatt ggtaatttat tttgtgctat ttgctcatat
     4141 atactatcat taacgataag tgttccaacg ctattggcaa aagaaatagc attgtttaat
     4201 gatgtttccc ttacagctac aggtgtttct ttaccattaa taagtaatgg ataaatcgtt
     4261 ccattttcac tatatccatc attatttggt tgatatttca ttaaaataca ttcattatcc
     4321 cttaaagggg taagttgttt tagtagcttt gttcgatttt gtgcctttaa taaagtataa
     4381 taatcagaaa aagatataca ttcaaatcct gattctcttg aaataacttc tttagaagaa
     4441 ctactattag ataaattatt cttagatgta ccaatttcat attcaaatgg aaggtctttt
     4501 gatgaagatt ttaccttata aatatttgtc tgtgtaaaaa taatatcatc tgtattagac
     4561 gtatattgac tgacaatttg ttttacatct tctatttgtt tatttttttc tactctaaat
     4621 tctatctctg aaggtgcaat acgcgaaaca gatgcaatag ggtagtataa ggacagtgcc
     4681 ataacacttg aaactgtcaa agtagtagca gagagtaatg taagcataat tagagtttta
     4741 gagtttgaac gtatacgata gataaagttt ggaatagcta ttatcttcat atctgtgtag
     4801 aaagtacgtt tatttttttt actcttttgc ataatatatg gaagaaagga attgataaag
     4861 agaactgtac caattacaat gataatcata gttatcaagc ccatttgata aaatccaata
     4921 cttacccata ctgatttagc tccacgaaga atatctagtg ataaacaata cccacttaaa
     4981 atcataataa atcccataaa ggctggaatt tttctgaact ttaatttttt ttctgcactt
     5041 ttttcatgtc ttatcaattt tacaagtgaa gtcttataaa gaaatttacc atttgatata
     5101 gctaagacta aaacaactaa tgctataaag ataattgttt tgtttattgc atttatatta
     5161 aaaaatggta tctgtgaatt atcaattgat aaattaagta gtgctgtaat tccaaagaca
     5221 atacccttat gtgacaatgc tcctaatacc aatccagtaa taaaagctcc actacaaatt
     5281 aatacatttt caaaggtaag tagcgataat attgtaaact tacgatagcc aagtaaagca
     5341 tatatgccta attcttttgt acgacgtctc aagaagaatc gatttgagta tgccatatag
     5401 aagatgacaa atgccatcaa aaatatcgaa ataacattac acatagattc aactctacca
     5461 tcagttgaaa ttttttctag cattatctta ttaacagaga atgaagtaaa tgcgaaaaat
     5521 acagtaataa caaaagagac tgaaagtaga tatagagaat agaatgaaaa gtttttttta
     5581 agattcttta ttgccatata aaaaatgttc attaaattat cctcctatct tttagtatcc
     5641 aattttgcaa tttcttgaat aatcaactca taaaatgtat gtctattatt cggttgttta
     5701 tttaattctt taatgatatg accatcttta aaaatcaaaa ttctatttgc aaaacttgct
     5761 gcataggcat catgtgttac cattaaaatt gttgttttca actcctcatt tgcttcctgt
     5821 agtgagtgga gtagttcagt agctgaatta gaatccaaag ctcctgttgg ttcatctgca
     5881 aagagaatac taggatgttt tactattgct ctaactgctg aagctctctg cctttgccca
     5941 ccagataatt catttgggta tttatttaat tgagaagcta tgtcaaatcg ttcagctaga
     6001 ctctttacca tgttttctat tttatctgga ttgagtttct gaagtgtaag aggtactgaa
     6061 atattctcga aaattgtaag actatctagt aaaaaatact cttgaaagat aaagcctaaa
     6121 ttatccttac gaaaatcagc agcacaagtg tcattaagcg atttcaaatt tatattattt
     6181 atgaaaatat ctccacttgt tggtttatca attgtagata atacattaag cagagttgtt
     6241 ttcccagagc cagaagctcc cattatacct ataaattcac cttctgatac attaaaggat
     6301 accttatcta aactcggctg ttgcccttta ttataagttt tagttatgtt atttactttt
     6361 aatattgtat tcattgtata tgtctccttt cgtcttaata tagtcaaatt atatattaac
     6421 cttattgttt cttcatgagt aaatcacgca ataattctac acaaattact tatatcacaa
     6481 tatattgttt aaaatctaac tttattaggt ttgggatgat ggacaagatt ttaatttaaa
     6541 agagtgcata gatatataat ggcataatgt agatttaaat taaaaaaaat aaaataaagc
     6601 cataaatatt gaaaaattca atatttatgg ctttattgct tgtaataatt aatagaatat
     6661 atatttttta ttaatcatct atttcaaatt ctaatatttt accagtaatt gcattaattg
     6721 ttatctcata ttcaattttt ccttttatta tttcaccttc atattctttg tcatcttcat
     6781 catacttaaa ttttactatt ttcccaccag gcactttatc tagcattatt gatttagctt
     6841 tttcagcact aataatagaa ctgttgtcat tagtattaga gttattctta tcattatcat
     6901 caaagtcatc atcagaatca ttgtccatgt caatatctac aattcttcca tcaaatgcac
     6961 ttatctctaa ttcatatgtt ttcttatttt ttattacttt agcctcatat tcgttatctt
     7021 cagcatcata tttacaatag attaaattac cattaggaac agcatctaat gctatagact
     7081 tagctttatc caatgatata ttttgtgtac ctaaattatt atcatttaag tcatcatctt
     7141 tttcaatttc tattatttta ccagagtagg catcaatttc tatctcatat aggatattat
     7201 cctttataac tttgcagtca tattcattat catcttcatc ataataacaa actaaaatat
     7261 cgcttcctgg aagagcttgt tttaatattt ctttagcctg tattaaagat atttttttag
     7321 ttttatttct tatgttcaca ttttttagtt ttaaaatatc ttcaaatgct ttttgattat
     7381 atcctcctcc tacttgtgtt atttcatcta tatctttatt tttaataaat tccttttgac
     7441 tatttacaag gctaccatta gatatcatta taggagaatt ttctttagca gctaatactc
     7501 ctattgataa tgaatctact aatgcatttt cttggttaat accatcttta gctacaaata
     7561 cattatttat ttttttatct ttataaaatt tatcaataat ttttgcatta gtattgtttc
     7621 tatctgaacc actgattctt tctctgtttg gatatttctt tactgtattg tctgataaaa
     7681 tcttatttcc tccaactatg taagattttt ttatagacct attgtttaag aaatttatac
     7741 tactatcatt gctgttttca tctgatacta atattatagg catattatgt attgctgctg
     7801 gagaagctat actaactgca tcagataatc cctttagtcc atttacaact gcaacatttt
     7861 ctaccttttt tatcttatct atttctttaa gtacatttgt agacatttta tatatattgt
     7921 ttcctactat tttatttatt tttatgttaa gtgattttat ttcattttct actttagaag
     7981 aaatgctatt tccactatct acaatgtaca cattatctac accaagcctt ttaagctcag
     8041 ccttagttga attttcaata ctgttgttgt ttactaaaag tataggtgca ttctttagct
     8101 tagcaaatgg atttgcacaa agcgcacttt gaatagaatt accattaact ataatggctg
     8161 tttttgattg aacccatccc aatttactta cttctactgc tgtttcatat cttgttttac
     8221 ctattaatct ttcttgcttt gtagtattgt ctgcaaaaat tgctgaagaa ctagaaaaaa
     8281 acatcatacc tgatattaat atagctaaat ttttattatt catattttta cctcacaatc
     8341 tttaaaaatt tgttatttag catacttaat aaaatataat cttaaaatca aattgatact
     8401 gagtttgaag ttacaaaatc aacaaataag atttctttaa tacaaaattt tcctttttat
     8461 atttaacatt gtatcacaaa tataaaaagg aaaaaacttt ttaaaaaggg tatacactat
     8521 tcatttttta tcttataaat aagtctttac cactgtttta tatattttaa aatagacaca
     8581 agacctattt ctaaatcttt tataaatatt ttaggtgaaa tttcttttta aacatatcta
     8641 aaaagtattt gtagtaatat taatggactg aatttcttaa agtatatatt tctttgcatg
     8701 ttggagtttg tgaagtttga gaattggagg gtgaaagaaa catgcaatgg attaatatta
     8761 aagaactgta tttttataat atttgtctta aaaataaaaa aatctattga cttgaagtta
     8821 actccaagta gtaaagtata attatacaag aattatatac ttgtaagaat agttaaagta
     8881 gagagaaaaa acaaacaagt tttaaagggg gaagtaaaac aatggaggca aaaaaattag
     8941 gatttggatt aatgcgttta ccaataaaag atgaaaatga tgttacaact attgatatgg
     9001 aacatttaaa taaaatggta gacacttttt tagaaagggg atttacatat tttgatacag
     9061 cctatgtgta tcacatggga aaaagtgaga tagcacttag agaatctcta gtaaaaagac
     9121 acaaaagaga gagttttact atagctacaa aacttccatt aatggcttta aagaaaaaag
     9181 aagagcaaga aaccattttt aatgagcaat tagaaaaatg tggtgtagac tattttgact
     9241 actatctttt acacaatata ggcgtgtcac attatgaagt agctaaaaaa tttgatagct
     9301 ttaagtttat tgaagaaaag aaaaaagaag gaaaaataaa gaatattggt ttttcttttc
     9361 atgatagtgc agaactattg gataaagtct tgagtgagca tccagaagta gacttcgtgc
     9421 aattacagat taattattta gattgggata atgaaagcat acaatctaga aaatgctacg
     9481 aggtggcaga gaagcataat aagcctgtaa ttgttatgga gccagtaaaa ggaggtacac
     9541 ttgcaaagat accagaaaaa gcagagaaat tattgaatga ctacaatcca gatatgtcaa
     9601 tttcatcttg ggcaatacgt tttgcagcaa gtaaagataa tgttatgatg gtcttaagtg
     9661 ggatgtcaaa tatggaacaa ttattagaca atacaggata tatgcaaaat ttcaaaccat
     9721 tcgtacaaga ggaatataat attatagatg aagcagtaga gattatcaac gaatcaattt
     9781 tagttccttg tactgcctgt cagtattgtg tagaaggctg tccaaaaaat atcgctattc
     9841 caaactattt tgcgctgtac aatgcagaaa aacaagctct taatactgga ttttcaacac
     9901 agatggttta ctataataat tatactaaaa cttatggaaa ggcctcagat tgtattgaat
     9961 gtaaacaatg tgaagaaagc tgtcctcaac acattaaaat tatagatgct ttaaagaatg
    10021 tagcagaaac ctttgagaaa taattcaatc tatacaatat caagaggaga agatatgtat
    10081 gacgatttca gaagttagta aaaaatacga gctttcagcg gatactttac gatattatga
    10141 acgaattggt ttaataccac ctgtaaatag aaataaaagt ggaattaggt cttttacaga
    10201 agaagattgt gagtgggtta atttcataaa gtgtatgaga ggtgcaggtc tttctattga
    10261 gacattaatt gagtatgtag ctatgtttca acaagggagc agtactatca aagctagaaa
    10321 agaactttta atagagcaga gaaaccaact agcaaaaaga atagaagaaa tgcaaaaaac
    10381 tttggaacga ttaaatttta agattgatag atatgaagaa ggtattattg agaaagaaaa
    10441 agtattaaag agcagtagaa ataagaaaat ggaggcagta tcttatgagt aaaaatgtat
    10501 taatagtatc agctagtcct agaaaaggtg gaaattctga cttattgtgt gaccagttta
    10561 tacaaggggt attagaatct aaaaatcaag tggagaagat atttttaaaa gataaagata
    10621 ttaactattg tactggatgt ggtgcttgtt atgaaaaaga agcaagttgt tcacagacag
    10681 atgacatggg agaaattctt gaaaaaatga ttggggcaga tgtgattgtt ttagcgactc
    10741 ccgtttattt ttatactatg aatgcgcaaa tgaaaactat gattgataga acttgctcta
    10801 gatatactga aatttgtgat aaagatttct attttatcgt tgcagcagca gatgaaaatg
    10861 tggctgatat ggaaagaaca atagaaggat ttagaggatt tacatcatgt ctaaatggtt
    10921 ctaaagaaaa aggtatagta tatggtgtag gtgcttggaa tgtaggtgat attaagggaa
    10981 gtaaggcaat gaaacaggca tatgaaatgg gtaagttggc ttagtatgtt tgtataaaat
    11041 tgtataaaat cgaatatatt cttatcattt caacaataaa aagaggctta tctatatttt
    11101 gagacagcct ctttgtgcta tatcatttat ttttattcta tatccttatt atacccttta
    11161 tttttttatg cctatttaag tatctgttgt tactcatata ttttaataat tacccttaaa
    11221 aaactataca gattcctgca taatattatt ttctttagta attaattttt ctaggttttt
    11281 ccaatccatt tcttccatat ttgcaaaata atgaccactt cctatgcaag tcagataaga
    11341 ctttccatct aaatcaaacg aagaacctcc aatacacttt gaattacatt tcttacaagt
    11401 acgagtttta gttatactat ctttaatagt tttagagcat tcctccacag catctttata
    11461 tgtatttaga tgaaaaagat ttgctttaac tctccatttt ttttcattac attcaatagt
    11521 aaataagcta cgttttggtt tcttagttga atatactatt ttgtaagaac gagcacctgt
    11581 ttttggttca attttacaat ctaatgattt accaagttca tgtaaatgac atgcaatatc
    11641 ataatcttta ccacttagta cttctaacat ttcttctgtt gtttctgccg ttaattcact
    11701 tttgctcata ttaaaaaaat ccccctatat aagtaataat ttcacatgaa aataattaat
    11761 ataagtatat taaataaaac tattaaaatc aataaaatta ctacaatctt acattattgg
    11821 gcatagaatg agagctaatg atttattaga aataagactt aatagtatga aaatttactt
    11881 agacgtggta caattctata aaagatttaa aactaggaaa ggagaaagtt gttgagatac
    11941 ggccaatata acaatttatt tataagttgt aaatttatta tgagattaat tagattgtta
    12001 agtaatacta tcaaaaggta gttttttgga ttttcagcaa cagtggtaga tattgataat
    12061 tttttggtta ttattatatt tagctgttat attttcaata ggctctatga taactagggt
    12121 aaataaggta tgggtaaagg ttttattatt tataatagtg ggattgtcaa ctatagttct
    12181 ttcatatgta ataacttgat aatttaagat tgaaaaataa ctttaatctg taaattctca
    12241 aaacaagaat tctcatgttg gtaaatttct agatatgaga aaaaaattga catcataaaa
    12301 aagtaatttt aaagcatttt tttacgaaaa aattcagaag taactatttt gaatgacttc
    12361 acaaaatttt atatataaat aaagtttggc acgttaattg ctttaataaa agttaacaag
    12421 acacaaataa acataatcat ctaagtattg attatttgaa gttttagatt tgaaatttaa
    12481 tctaaaggtt cgtattaata ttggtgttta ggaaatttta ttttcctgta aatataactt
    12541 aataatagga ggtgaagtca tgtcaaaagt agtaatagct acagagaaag ctccaggagc
    12601 aattggacca tattctcaag caattcaagt tggaaacata gtttctacat caggtcaact
    12661 tccaatagat atgaagactg gcgagttagt atctgaccca aaagaagcaa caaaagcttc
    12721 tttagataat gtaaaagcaa tattagaaca agctggtaca agtatggata aaatatttaa
    12781 gactacagta ttcgtaaaag atttaaagga ttttaatgat attaatgaag tatatgctac
    12841 atattttaaa gaaaatccac cagcaaggtc atgcgtacaa gttgctcaat taccaaaaga
    12901 tgcagtttta gaaattgaag caatggcagt attataagta ttataacaat aaaaccaata
    12961 acaaaactaa taaaaattaa aaactaataa aaaaagtcta caattaaaaa agtttatatt
    13021 aaaaaagtct atataaaaat ctatctaaat atttaactat tgagcttttc tcatataatg
    13081 tatattttta tgtcaaaaat atatttgtta tatgaggaaa gcttttttta ttttattatt
    13141 gtattgtgat ttactttatt gtctgtatct tataattaag taattaaggt catcatataa
    13201 aacaaacttt ttataaatta atattgtgtg ttttaatgta aatatataaa taagtaatgt
    13261 tttatatata ttttaaagag tgactatatg ttaaaatata gctaaactga taatttggta
    13321 aattaaattc taatggggga aactatgtta agtaaattaa aagaatttca acaagaaatg
    13381 ataaaataca ctgaaacagt agctagtgtg ttagatgtag atattgaaat agttgatgat
    13441 agattaataa gaattagtgg tacaggtctg tataaatcaa agataaatga aagcgttgtt
    13501 acagaaggat ttatttacga caatgttata cagacaggtc aggaattagt cgtattggat
    13561 atatgtgata atcagctttg tatagaatgt tcccattaca tgaaatgcct aaataaagta
    13621 ataatagcag ttccaataaa gtataataat cgaactatag gagtaattgg tgctattagt
    13681 acagataaaa ctaaaaaagt agaaataagt gcaaaaatag ataattattt aaaatttgtc
    13741 aatcatatat gcgatttaat ttctatgaaa atagaagaac atgaagcgtc aaaaaatagc
    13801 agtagaaaaa tggatatgat gatagaaatc attgaaaatg ttgaaaaagg cgttataata
    13861 ctagacataa atagcaaaat atcttatata aacaatatag ctctaaaaaa attagatata
    13921 tataaaaata taatagaaaa tatagtaaat atagtatctg ttgaatcttc ctcaaatgga
    13981 catgaattac tagaaattga tattgataac aaaatttata atataaatgc caaaataatt
    14041 ccggtatatc catatataaa tcaatacgac aaaataataa tatttgacaa gacatatatc
    14101 aatcataaag gacatggaaa agtcaatagt ggctggggaa atagtgatat agagtctata
    14161 attggaaatt cagaggcaat gttaaaagta aaagagagaa ctaagaaatt agcaaagtca
    14221 aattcaactg tacttataac tggggaaagt ggtacgggta aagagctaat tgctagggct
    14281 atacatgctg aaggtagcag atggaacaag ccatttattg cgataaactg tgctgctatt
    14341 ccagaaaatc tccttgaaag tgagctattt ggctatataa aaggtgcatt tagtggagct
    14401 agtagtggag gtaaagtagg taaatttgaa cttgcaaatg aaggtgttat atttcttgat
    14461 gaaataggag atttgtcaat gcctttacaa gcaaagttac ttagagtttt acaagagaga
    14521 aaatttgcga gaattgggtc aaataaactt atagatttag atataagagt gattgctgct
    14581 acaaataaaa acttattgaa attagtaaat gaaggtaagt ttagggatga tttatactat
    14641 agattaaatg taattcctat aaatttgcca cctcttaggg aaagaaagga tgatattgag
    14701 gcaataatga tgaagtttgc ttctaaatat tctttagaat taggtattca gttaaataaa
    14761 atagaagaaa atgtaatgaa tatgttgatt aactataatt ggccgggaaa tataagggaa
    14821 ctggaaaatg cagttgaata tatgatgaat ttggttggag atgatggaat aatatataaa
    14881 gatatgcttc cattggatat tttaaattat tataatatta atggaaatgt ttgtaaaaat
    14941 aaggatataa atataatatt tgaagatgac atagttggag ggatagtaga gaatcaggaa
    15001 aggatacttt caattaaaga attagagttg acttacataa acaagttgtt gaataagtat
    15061 ggaagagata caaagacgaa gaagaagata gcaaaggatt taggtatagg tttggctaca
    15121 ctatatagaa aattggaaga ggaacagtag taatataata ttaaaatgat aggtatttgt
    15181 gtgtttttat agataggtat ttttacttta tggtggaggt gttattatgc aatatgcgat
    15241 agagctatat tatgataaaa aaacggaaaa gcaacttttt gatttatcta aaaaaatcgc
    15301 tgatgaaaaa attagtacga agtacttaga atggaaaaca agacctcatc ttactttggc
    15361 ttgtttcaat gatgtggatg aggcttgttg catagacaag ctaaaggggt ttgcacaaaa
    15421 ccataaggta atacctgctt acattggttc agtaggcatg tttaatgata ctaaaacggt
    15481 gtttgtatca cctataatga atagcagtat gtatcagttt cagagagaat tatatgaggt
    15541 actacaagat tttgattcaa atggttggga atggtaccac cccaatcgtt gggtacccca
    15601 ttgcacaata gcacttactg gagatgatga aagtgaggct ttttttcggg caagtgatgt
    15661 attactgcgt gagttcaaaa aaataagtgg tgagtttgtc tcaataggat tggttaaaat
    15721 tacatttcct gtggaagaaa tttttacagt gaatatgaat aggtaacttt aactctgttg
    15781 tggatgttat gaatattttg catagtgcat ttcatcagat aaatgcgaag ttgcgaattt
    15841 cgacagctca atagtcgcaa gtaggacaca atggtacaca tattgcactt ttgcttattg
    15901 gagggacgcc catgtaaact tctgtaagaa gctatgaaaa gatttaagcc attttggagg
    15961 ctttgggtaa taaagaatat ttgggtaata aagaatataa agtatataaa tttatagagt
    16021 ttatgcgtaa gaaggttata gccatatatt tatagaaaat aaaagtagta atatatctga
    16081 caaaacaata gacatataaa aaataagagc ataataaagg aagaattaaa ataaataaac
    16141 gatttctcga attgataatt ctcgtattga taaaaatttc ccaatatgag aattttttct
    16201 tttataaatc ttatttttca catttaatat ttataaaatt caaagaaatt ttataaatat
    16261 atgtaattcc aacataaaaa aattgttttt agaataaata atattggcat agtaattgct
    16321 tcactataag acacaaagat aaaaaattaa aatgacaccg aaaaatttga aaggggaata
    16381 taaaataaat gagagaaata aaatggaaat taaataactt acctaaatca gaggaaaaag
    16441 aaaaaggaat tgagttttta agagatgaag aaattgcaaa ggctaaagct tttcatgaga
    16501 gctttccaca atatgaaaaa actcctttag ttaaattaga taacttagca aaatcactag
    16561 gcgtaagcgg aatatatgtt aaagacgaat cttatagatt tggtttaaac gcatttaaag
    16621 ttttaggtgg ttcttactct atgggtagat acttagctca aagattagat acagatatat
    16681 ctgagttagg gtatgacaaa ttaacttcaa aagaaataaa agaaaaatta ggagaaataa
    16741 ctttctttac agccacagat ggaaatcatg gtagaggagt tgcttggaca gctaataaat
    16801 taggtcaaaa atctgttgta ttaatgccaa aaggttcttc tgaatttaga ttaaataaaa
    16861 tcaaaggaga aggtgcagat gctagtataa cagacttaaa ctatgatgat gcagtaagat
    16921 tagctaatga ttatgcagaa gctgatgacc atggagtaat ggttcaagat acagcatggg
    16981 acggatatga agaaatacca gcatggataa tgcaaggata tggaactatg gctaaagaag
    17041 ctatagaaca attaaaagaa tatggagtag atagaccaac acacgtattt gttcaagcag
    17101 gagttggttc acttgctggt gcagttcaag ggtatgttgc ttcaatatat gatgaatgcc
    17161 caataacagt agtagtagag gctgatgagg ctgattgtta ctataaatca gcagaagctg
    17221 gagatggaaa accaagattt gttggaggag atatgccaac tataatggca gggcttgctt
    17281 gtggagaacc aaacactata ggttttgaag tgttaaaaaa ttattcatca gcattcgttt
    17341 cagcaccaga ctgggtatct gctaaaggta tgagaatatt aggaaaccct ctaagaggag
    17401 atgagaaagt aatctctgga gaatctggag cagttacaac tggagcatta gtttcaatcc
    17461 ttgaaagtga agatttaaaa gacttaagag aagctttgaa actagatgag aactctaaag
    17521 tattattaat aagtacagaa ggagatactg accctgataa atatagagat atagtttgga
    17581 atggtgaatg ccaaagtaaa taaaagactt aaggaaaata aaaacttaac aaataaatga
    17641 aaaatttaag gaaaataaaa acttaaataa gtgaaaactt taaataaaat aaaaactaaa
    17701 acaaatgaaa aatctaaata aatgaaaaag taaagcaaat gaaaaattca aaataaaaac
    17761 taaataaaat taaataaaac caaataaaat taaaataaag tctaaataaa aatttaataa
    17821 ataaaatata aagaaataaa aaatcaatag attaagtgaa atgctaataa attaaaaaag
    17881 tgaaatatca aaataaaacg tcaacaaatt aaaaattata tgggggaata tcaaaatgtt
    17941 aaatgaaaca agaaaaaaac aattaactga agtatgccaa gacttaataa gaaatgctag
    18001 ttattcaggt caagaagaaa acgtagtaaa agctatagaa gaaaattttg ctttactagg
    18061 ttttgatagt tggtcaagag atagatatgg aaatatagta ggatgtataa aaggaaataa
    18121 accaggtaaa aaaatattat ttgacgggca tatagataca gttccagttc cagatgcaag
    18181 taaatggagt gttcctcctt ttgaagcgag aatagtagat gggaaaatat atggtagagg
    18241 aacttctgat atgaaggggc aagtaagtgc tatgatggct gctgtttcat actttgcaga
    18301 agatactaac aaagactttg aaggtgaatt gtatgtagca ggtgttgttc atgaagaaat
    18361 atttgaaggt gtttcagcta gagagataag taaggctgtt caacctgatt atgttgtaat
    18421 aggtgaatct tcagaattaa acttaaagat aggtcaaaga ggtagaggag aaatagtagt
    18481 agaaactttt ggtaaaccag ctcactctgc taatccagaa aaaggtataa atgcagttta
    18541 taaaatggct aatgtaatcc aaaagataca acaattagta cctccaactc atccagtttt
    18601 aggtgatgga atattagttt taacagatat aaaatcttct ccatatccag gagcatcagt
    18661 agtgccagat tattgcaaag ctacttttga tagaagatta ttagttggag aaacaagaga
    18721 aggcgtatta gctccaatac aagctttatt agatgaaatg atgaaagaag acccagaatt
    18781 aaatgcaaaa gtttcttatg cagttgaaaa agctgattgc tatacaggaa atactataga
    18841 aagtgaaaga ttcttcccag gttggttata tgatgaagaa gatgaatttg ttcaagcagc
    18901 gtataatgga ttaaaagaag ctggaataga ttcagaaata actcaatact cattctgtac
    18961 gaatggaagt cactacgcag gtgaggctgg aataagaact ataggatttg gtccttctaa
    19021 ggaaaatctt gctcatacaa tagatgagta tatagaatta gaccaattat tcaaaggtgc
    19081 agaaggatat tatggtatat taaaatcagt ttataaaaaa taattaggag atggttttgt
    19141 gagaacttta ataaaacatg ggcttatagt agatggaaat aaaacaccag catatgaagg
    19201 agatatttta atagaaaacg agaaaatatt aaaaatatca caagatttaa acgaagaagc
    19261 tgacaaagtt atagatgcaa aaggtagagt tatatgtcca ggtttcatag atactcatag
    19321 ccattcagac ctagttattt tagttaatcc atataatgag gtaaagatta gacagggaat
    19381 tacaacagaa gtgttgggcc aagatggtat atctatggcc cctctacctc aagagcatat
    19441 atcttcttgg agaaaaaatt tagcaggtct agatggtgaa agtgatgaaa ttgattggaa
    19501 atatgaaaca acagagaatt atttaaaaat gatggattac aatggggtag gtttaaatga
    19561 aacttattta gtacctcatg gaaacgttag aatggaagct atgggacttg aagatagacc
    19621 tgctactaaa gaagaaattc aaaaaatgtg tgaaatcaca gagagagaat taaaagcagg
    19681 agctatagga ttgtctactg gacttatata tataccttgt gcatactcat tgactgaaga
    19741 aattatagag atgtgtaagg tagtagcaaa atatgacgga gtatttgtag ttcaccaaag
    19801 aagtgaagca gatacaattt taacttcaat ggaagaaatc atagaaattg gaaaacaatc
    19861 aggagtaaaa gttcattttt cacattttaa ggtttgtggt aaagcaaact ggaagtatat
    19921 accacaagtt atagagcttt tagaaaaagc agaaaaggaa ggaataagag tatcttttga
    19981 ccaatatcca tatgcagcag gaagtacaat gcttggagta gttttacctc cttgggcaca
    20041 ttctggagga actgataagt taatagaaag attaagcgat gaaaacgaaa gagcaaaaat
    20101 gaaaaaagat atagcaaatg gaatagaagg ctgggataat tttatagaat ttgctggaat
    20161 agaccaaata tttgtaacaa gcgtaaaaac agaaaagaat aaagatacaa ttggtaagag
    20221 cttattagaa attggaaaaa tgagaggaaa agacccatta gatgcaacgt ttgatttatt
    20281 aaaagaagaa gaaaatgcag ttgggatggt agatttctat ggattagaag aacatataat
    20341 aggattcatg aaaagagacg agcaaaatgt gtgtacagat ggtcttttag caggtaaacc
    20401 acatccaaga gcttatggtt ctttccctaa aatactagga agatatgtta gagagttgaa
    20461 tgtactaaca attgaagaag ctgtttataa aatgactaaa aaggcagcta caagctttag
    20521 tataaaagat agaggcgaat taaaagaagg atattttgca gatatagtta tatttgacaa
    20581 ggatacagtt agtggttgtg atgattttat caactctatg cagtacccta caggtataga
    20641 ttacgtaatt ataaatggaa attgtgttgt tgaagaagga aaatacaata aaattaaagc
    20701 aggaaaagta cttaaaaatt aaatcttagc tttaaggagg aataaattta tgattttagt
    20761 ggggaatggg aggctaataa gccaagattc tttaaatccc tatatggaag atggatgtgt
    20821 agtaataaat gataatctaa ttgaagatat aggtactaca gaagaaatga aagctaaata
    20881 tccagtacat gaatttatag atgcaggcaa gaaaataatc atgccgggtc taataaattc
    20941 acatatgcat atatacagct catttgctag aggaatggca gttccaggaa aaccttctga
    21001 gaacttttta gagatactaa ataatttatg gtggagactt gacaaacagc ttacactgga
    21061 agatacaaaa tacagtgcat atgcaacgta tatagaatgc ataaaaaatg gtgtaacaac
    21121 agtttatgac catcatgcaa gtccaaatgc aattgaaggc agtttgttta caatatcaga
    21181 tgttgcaaag gatttgggaa ttagaacgtg tctttgttat gaagtgtctg atagagatgg
    21241 tggaaataca atccaacaag gtatcaatga aaacgtaaac tttataaaat acgctcaaaa
    21301 agatgataca gacatgataa aaggaatgtt tgggcttcat gctgcattta cactatctga
    21361 tgatacatta aataaatgtg cttcagagat ggctggtctt gatacaggtt accatgtaca
    21421 tgttgctgaa ggactagcag acttacaata taatgtagat aaatatggaa aaagggtttt
    21481 agaaagattc aatgattttg gagtactaaa agaaaaaaca atagcagttc attgtatata
    21541 tataagtgca gaagaaatca gtcttttaaa taaaacaaat acatttgtag ttcataatcc
    21601 agaatctaat atgggaaatg cagttggatg ttctcctgca atagagttat taaaaagggg
    21661 agttacagta ggattaggaa ctgatggata tactagcgat atgtttgaat cattaaaagt
    21721 tgccaatata attcacaaac atcacttaaa agacccaaga gttggatttg aagaatcaca
    21781 aacaatgtta tttgataaca atagaaaaat agctaaagct tattataaaa atgacctagg
    21841 agtacttaaa aaaggtgctt atgcagatgt tatagtagtt gattatatac cacatactcc
    21901 attgacagaa aatacaatag ggggacacac tatttttgga atgagtggaa gaagcgtaga
    21961 tacaactatt ataaatggaa aagtacttat gaaagataga aagttaataa acatagatga
    22021 agaaagtatt ctagcaaaaa gtagagaatt aagtcaaaat ctatggaata gaatttaaat
    22081 aattgattat gcaaaattct atgaagcagg aaccctgtga ctttagtcat gggaggttca
    22141 gattaatata caagtaagaa acaagaattt agatgttaaa aacagagctt aaacaaaaat
    22201 aggaatttaa atgaagtata taaataaaaa atatacggaa tagaatatta aattaaatat
    22261 agaagggaga cccaaaatgt ctcaagataa aagccctaaa atagctccag tagatgaaag
    22321 gttacctgta tccaaatctt gggtattttc actacagcac gttttagcaa tgtgtgcagg
    22381 agcagtagca gttcctatga tgataggaag tgctgcacga ctagatcatg ctagtattgt
    22441 ttttttaata aatgcttgct tgtttatggc aggtatagga acactcatac aaagtgttgg
    22501 tataggaaat attgtaggtg ctaaaatacc agtaatagaa ggaactagtt ttgccgctgt
    22561 ggcaggtatg acagcaatag cctcatcata tagtgattca caagttgcaa tacagacagt
    22621 atttggctct gtaatagtag ctggattgtt ttgtatatta cttgctccag tatttgaaaa
    22681 attaataaga tttttcccta gtgtagttac tggaacagtt gttatggtaa taggtatttc
    22741 actatttcca gttgggataa aatggataac tgacaacaaa ttggaagcgg ttgaaccaaa
    22801 ctcattagca ttagctttag cagtattggc aataacttta ctttcattta aattcttaaa
    22861 aggaatttgg aatagcacag ctatattggt aggtatagta cttggtacac tactttccat
    22921 ggcagttgga atggcagatt ttagtgcagt aaatggagtt ggttggctta acttaaatgt
    22981 acctttaaaa tttggaatgc ctagatttga accatctgca atactatcat tgtgtttgat
    23041 tatgttggta cttatgacgg aatcagttgg taatatgata gctattcatg aaatggtaga
    23101 caaagaagta acaggtaaaa atattaaaag aggtctattt ggagatggaa tatctacagc
    23161 attatctgga atattcaatt catttccaat aactccattt ggtcaaaata caggaatagt
    23221 aggtcttaca ggtataaaat caagatttat agcagtttac gcaggggtta tattgctagt
    23281 acttggattc ttaccaaaat ttgcagcaat aataggagca atacctaagc ctgttctcgg
    23341 aggcgtagga tttgcaatgt ttggaatggt aatggttgga ggagtaaaaa ctttaagcaa
    23401 agtgaaattt gatggaacta aaaatggggt tatagtagca gttagtgttg gtttgtctat
    23461 gataccacta acaaatcccg atttctatca taatttccca acttgggtac aaacaatatt
    23521 ccatagtgga ataacaacag gtagtttatc agcagtgctt ttaaacatat tcttcaatga
    23581 actaggttct aaaaaagaaa aaaataataa agttgaagaa gcaaaataaa aataaaacaa
    23641 gtaaaaatct atattgactt aaagtcatta agcattaata aaaataattc agagatatta
    23701 ataattcaac aaaactatct tatattttaa ataatataaa aaagtaaata aataacgaat
    23761 acaaataaaa gaatacaatc taaatattaa aaatatttaa tttaagggag agataataat
    23821 atggctaatt taaaagttaa tttatatgga atggaatttt taaacccaat tatgacagcg
    23881 gcaggacctg gagcaaaaga tggtgctctc tgtcaagagg cagcaaaagg tggagcagga
    23941 gctatatgta caaaaactat atctgtatta ccagctgatg taccaagacc atgtatggca
    24001 aaaactaata gtggtttttt aaacacagaa ttatggtcag agcttccaaa agagcaatgg
    24061 atagaggaag aatacaaaaa ggtaaaagaa acagggcttc cagtaatagt aagtatggga
    24121 tattcagcag agcaaatagc tgaggttgct cctttagtaa agccttatgc agatgcagtt
    24181 gaattatcta ctcattatgt aggtacagac gttgcaccaa tagtaaatgc tttaaaagct
    24241 gcaaaagctg ttttagatgt accagtattt atgaaaatga gtcctcatac tgacattcaa
    24301 actattgcaa aagctgttga agaagcaggt gcagatggac ttgtaatgat aaactcattt
    24361 ggaccatgta tggcaataga tgtaaataca ggatacccta taatgggaag taaagctgga
    24421 tacggatggt tatcaggtgc accaataaga cctctagcaa taagatgtat atatgaagct
    24481 tctcaagttg taaatatacc aataatcggt gttggtggaa tcacttgtgg aaaagacgtg
    24541 gctgaaatga tgatggctgg agcaagtgct gttcaagtat gtacagaagc aatccttaaa
    24601 gggccttcta tctatggaaa aattgctaaa gaattaaatg aatttttaga tgagaatgga
    24661 tatgctgatg ttaatgaaat aaaaggatta actcataaga aaatggcagc tagagggttt
    24721 agaactcatt cagtagctcc agtagtggat gataacaagt gcatcaaatg taagcaatgt
    24781 gtaacaagct gtgtatacga agctttagaa gttacagata agttgaacat agatgcagac
    24841 aagtgttttg gatgtggatt atgtgtaact agatgtccaa aaggagcttt aagcatctgc
    24901 tacaatcaag tagaagctat gaatgagtaa taattatata gaattttggt aattaactta
    24961 gagtaatagg tattaggata ataaaatata tcagataaac aaacacacat tatgcattaa
    25021 taatacagtc taaagttaat aattttatta tattgattta actaaatatt agtttagctg
    25081 aatattaatt taattaggta ttggtttaat cttaatatag atttactacg tataagtgaa
    25141 ctaactagat tactttaagt taattttttc atataaaata aattgataca ttacaagtaa
    25201 ggtggagtct atatgggaat tgttttaaaa ggtgggaaag tagtaacaag tgaaaaaact
    25261 tactgtgcag atgtaagaat agaagatgaa aaaatagtcg atataggtct tgatatatca
    25321 aaagaaaatg atgaaatagt tgatgttagt gattcttatg ttataccagg aggtatagat
    25381 acacacacac actttgactt agatacaggc gttactaaga cagcagataa ctttgagaca
    25441 ggaacaaaag ctgctatagt aggaggaact actactatat tggattttgc tacacaagct
    25501 aaaggatgtt ctttgaaaga cggattagaa gaatggcatg aaaaagctaa ggataaatcc
    25561 tattcagatt atggctttca tatggctata acagattgga atgactcggt ttgtaatgaa
    25621 atggaggata tggtgaaaga gggagtatct tcatttaaac tgtatatggc gtataaagga
    25681 agccttcaag ttgatgatgg tgtaatcttt gaagcattga gaaaagcaga agagatagga
    25741 gggattattg gtttccattg tgaaaatggg gacataatat gtgaacttgt ggataaagca
    25801 aagtcagaaa ataatttatc tccaaaatat catcaattaa ctagaccagc tataatggag
    25861 gcagaagcta catcaagact tatgaagata gctgaagttg caaactcaaa agtatatgta
    25921 gttcacttaa gctgtaaaga atccttagag gaggttttaa aagctaagca aagaggtgtg
    25981 gatgtaatag ttgaaacttg tcctcaatac cttctattaa atgatgaatt atatgattta
    26041 gatggatttg aaagtgctaa gtatgtaatg tcaccacctt taagaaataa agaaaataat
    26101 gaaatattat ggaatgctat tttagataat caaatagaca ctataggaac agaccattgt
    26161 tcttttaatt ataagggaca gaaggaactt gggataaacg atttcagtaa gataccaaat
    26221 ggttctccag gtgtagaaca taggatgagt cttttataca catatggagt ctgtaaaaat
    26281 aaaatatcta tcaacaagat ggtagaagta acatcaacta atgcagcaaa gttgtttgga
    26341 atgtatccta ataaaggaac tatagaaata ggcagtgatg cagatatatt agtcttaaat
    26401 ccaaacaaaa aatccaaaat agaagcaaaa aaacaattac aaaatgtaga ttatacacct
    26461 tatgaagggt atgaagtgga ttgtcaaata gaacatgttt atttaagagg aaaagaagta
    26521 gttagagatg gagaattaat ttgtaatact ccaagtggga gatatataaa taggcgtcta
    26581 acaaaggggg ctagagaatg gtagaattta tattaaatgg agagaaagtt gttgcaaaag
    26641 aaggcaaaaa acttttagac ttccttagag aagatatgaa tataacttca gttaaaaatg
    26701 gctgttcaga aggtgcttgt ggaacatgta tggttataat agatggtaaa gctactaaag
    26761 catgtgtatt taaaactgat aaattagctg ggaaaaacat aacaactata gagggattat
    26821 cagagagaga aagagatgtt tttgcatatt cttttgctag tcaaggagct gtacaatgtg
    26881 gattttgtat tccaggtatg gtaattagtt caaaagcact tatagatgtt aataataatc
    26941 ctactgaaga tgatataaag aaagctttaa aaggtaatat atgtagatgt acagggtatg
    27001 taaaaataat taaagctgtc gatttagcag caaagatttt tagagaaaat atagatgtgc
    27061 caaaagtaaa ctgtaagggt ctagtaggtg aaaatcttcc tagagttgat gcagttggca
    27121 aagcacttgg atattctgaa tatgtagatg atatgagaat tgagggtatg atttatggtt
    27181 cagcagttag agctaagtat cctagagctt tagttaaaaa aatagatata gaagaagcaa
    27241 aagcttatcc aggagttgtt gcagttctaa cagctaaaga tattccagga actataaagg
    27301 tagggcactt aaagaaggat tgggatactt taataccaga aggtgaaata actagatatt
    27361 taggagatgg aatagtttta gtagcagcag aaactagaga agcgttagaa gaggccaaaa
    27421 agctagttaa gatagattat gaggaattaa caccattaag tactacagca gatgcactta
    27481 gagaagatgc tccaaagata catgagacag ggaatatact tgttaaggaa catttagtaa
    27541 gaggaaacgc taaagaaaag atagaaaatt caaaatatgt tgtaactaaa agatatacta
    27601 cacctgctac agaacacgca tttttagagc ctgaatgtgc agtagctggt ccttatgaag
    27661 atgatggagt aataatctat tcaacagacc aaggggtgtt tgccactcaa catgaatgtg
    27721 cagatatgtt agggttacca tatgagaagg taagagttat aaacaaaatg gtaggaggag
    27781 ggtttggtgg aaaagaagac atgagtgttc aacaccatgc agccttactt gcataccata
    27841 caaagagatt tgtaaaagta cagttaacaa gaaaagaaag tattattgtt catccaaaac
    27901 gacatgccac agaaatggaa tttacgacag catgtgatga aaatggatac ttaacgggta
    27961 tgaaggcaac gataatatct gacacaggtg catatgcctc tcttggagga ccagtgctac
    28021 aaagactgtg tactcatgca gcaggaccat acaattatca agatattgat ataacaggaa
    28081 cagcagtata tactaataac cctcctggag gagcatttag aggatttgga gttacacaga
    28141 gttgttttgc aactgaatgt aatctaaatt tacttgctga aatggttgga atatctcctt
    28201 gggagataag atataaaaat gcaataagac ctgggcaagt tttaccaaat ggtcaatttg
    28261 cagatgaagg aacagcaatg gcagagacac tagaagctgt aaaagaagag tatgaaaagc
    28321 atccacgtgc aggtatagct tgtgctatga aaaatagtgg ggttggagtt ggtatacctg
    28381 atgttggaag atgtagattg gtgataaaag atggcaaagt tcacataaga acaagtgctt
    28441 cttgtatagg tcaagggctt ggtactgtta tgactcaagt agtttgtgaa actgctagcc
    28501 tacttcctga aaatatagtg catgaagttc cagatactca tttaacacca gactctggta
    28561 atactacggc ttcaagacag acagtattta cgggagaagc tactaaggtt gcatcaaata
    28621 agttaaaaga agctttagac tctggcaaaa ctttacttga tttagaggga gaagagtttt
    28681 atggagaata tgaaagtatt acagataaaa tgggttgtga caaagaaaat ccagtaagtc
    28741 atgttgctta tggatatgca acacaagttg taattctgga tgaaaatgga aaactagaaa
    28801 aagtagtagc agcacatgat gttggaagag ctataaatcc aatcagttta gaaggtcaaa
    28861 tagaaggtgg agtagttatg ggtcttggat atgctctaac cgaagattac ccagtagtta
    28921 actctgttcc tacagctaag tttggtacat taggtttatt taaatctaca gatataccag
    28981 aaatacaagc tttaatagtt gaaaagaaca aagatttatt ggcttatggg gcaaaaggtg
    29041 taggagaaat ttgtacagtt cctactgcac cagcagtaca aaatgcctat tatgtatttg
    29101 ataaagaatt tagaactgag ctaccactac aaaatactcc atatagtaaa aaaaagtaac
    29161 aaaaaaaaga aatattaaat aaaaaaatag atgaaattgt aaataaaaaa aacccttttg
    29221 cataaggtat attttaaatg atagaatata catatacagg agggtttttc ttttataaaa
    29281 taagtttaat aaaattgaga ggtgagcaga atgaatttta ataatagtct agaattaact
    29341 cagtctcaga agctaataat gactacacaa cttaagcaat cattatcaat attaaatatg
    29401 agtaagttag agttagaaga agagataaaa agagaagcag agaataaccc attagttgaa
    29461 gtcgagaaaa gtggggagat aaattgggaa gagtatataa aagacatgga taggtcaaga
    29521 cgtttagata gaacagagat aagctataat cctgataatg aagttaatct agaaaattta
    29581 gttaaatatt cgtcaaattt atatgaagat ttaaaatttc agataagcct gtataaactt
    29641 acagacaaag aattggaagt ctgtgagtat ataattgata gtttagatga ggatggatat
    29701 ttaagaactg aggaaaaagt aattgttgat accttaaaga tagatgaaga actttttgaa
    29761 aaatgtttaa ttagtgttca gcagttggaa cctagtggag taggggcaag aagtctttca
    29821 gaatgtctga tgatacaaat ggctagttta ggaatatata atgaaatttt agaggaaatc
    29881 gttacaaaag acttaaattt aattgcaaat aataaatata aagagataag taaaaagtac
    29941 aatatgtcat tacaaaaatg tgttgaacta ataaacataa ttaagacatt agaccccaaa
    30001 ccaggaagaa cttgttcagt agagaaaagt gtatacattc aacctgatgt aacagtagaa
    30061 aagattgatg atgaatttat tgtatattta aatgagaaag attcttacca aataagaatc
    30121 aatagttatt ataaagacat attaaaaaat tcacagtctg atgaaagtgc aaaagaattt
    30181 ataaaagaaa gactaaattc agcaacagga cttatgaaaa atatagagag taggaaaact
    30241 acagtactta agattgcaga agagatagta aaagctcaag atgaattttt gagaaaaggg
    30301 acaaaatata taaaaccttt gaaaatgaaa gacatagcag aaaaattaga atttcatgaa
    30361 tctactataa gtagaggtgt aaatggtaaa tatatgctaa caccttttgg cgtatatgaa
    30421 tttaggtact ttttcagtag cgccattgag actgagaata atgaaatggc atctagtaca
    30481 agcataaaaa aaattataaa ggaaacaata aaagacgaaa ataaaaagaa acctttaagt
    30541 gatgaccata tatctaagtt attaaaagaa aaaggaatca atgttgcaag aaggacagtt
    30601 gccaaatatc gtgaagaatt aggaatccca tcttcaagta agagaaaaga atactaataa
    30661 gtaaaagaca aatatcagta tggtaagggg aaaaggctaa ttaagagatg aatgtagatt
    30721 gggctaaaaa ttttataaag taaaataata gaatatggaa aagtattaaa atgctataag
    30781 gtattaaaaa actggggaac attaaaacat taagatagca aagaacataa aaaactaaag
    30841 aagattaaaa cattaaagaa cattaggctt tgaggaatat tagaacttta aagaatatta
    30901 aaatttataa aatataaaaa tactaaaaag aattgaaaaa ctggggaaca ttaaaatatt
    30961 gtaaaatgct aaaaaactag aaaacataat taaataagtg cttataaaga atgattaaat
    31021 taaataagtg tttgtaaaga ataattagat attagaaaaa gaagaatacc tagataaata
    31081 aactattatt aaatttaata atcacaaaag gaaaacacgt agggtataaa taaaaatctt
    31141 aactagataa aagtaaactt agttgtagat aaaaataact agaaataaaa gtaactagaa
    31201 at
//
LOCUS       DAMJVX010000025        30669 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMJVX010000025 DAMJVX010000000
VERSION     DAMJVX010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30669
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.027"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            124..2025
                     /locus_tag="PC407_001129"
     CDS             124..2025
                     /locus_tag="PC407_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2496587.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASNLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            2028..3071
                     /gene="ypdE"
                     /locus_tag="PC407_001130"
     CDS             2028..3071
                     /gene="ypdE"
                     /locus_tag="PC407_001130"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2496588.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            3086..3391
                     /locus_tag="PC407_001131"
     CDS             3086..3391
                     /locus_tag="PC407_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2496589.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPTMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            3488..3823
                     /locus_tag="PC407_001132"
     CDS             3488..3823
                     /locus_tag="PC407_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2496590.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            3855..5177
                     /locus_tag="PC407_001133"
     CDS             3855..5177
                     /locus_tag="PC407_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2496591.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            5187..6257
                     /locus_tag="PC407_001134"
     CDS             5187..6257
                     /locus_tag="PC407_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2496592.1"
                     /translation="MNNTIERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            6262..7449
                     /locus_tag="PC407_001135"
     CDS             6262..7449
                     /locus_tag="PC407_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2496593.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVANNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(7646..8518)
                     /locus_tag="PC407_001136"
     CDS             complement(7646..8518)
                     /locus_tag="PC407_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDF2496594.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVSYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYKQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLHLNPEVFKNDALIYTNIDEVSIYNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(8912..9103)
                     /locus_tag="PC407_001137"
     CDS             complement(8912..9103)
                     /locus_tag="PC407_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496595.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            9323..10369
                     /gene="cobT"
                     /locus_tag="PC407_001138"
     CDS             9323..10369
                     /gene="cobT"
                     /locus_tag="PC407_001138"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDF2496596.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            10401..10961
                     /gene="cobU"
                     /locus_tag="PC407_001139"
     CDS             10401..10961
                     /gene="cobU"
                     /locus_tag="PC407_001139"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDF2496597.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            10971..11744
                     /gene="cobS"
                     /locus_tag="PC407_001140"
     CDS             10971..11744
                     /gene="cobS"
                     /locus_tag="PC407_001140"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDF2496598.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWIIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            11764..12378
                     /locus_tag="PC407_001141"
     CDS             11764..12378
                     /locus_tag="PC407_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2496599.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      12445..12624
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12754..14253
                     /locus_tag="PC407_001142"
     CDS             12754..14253
                     /locus_tag="PC407_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HDF2496600.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVGVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            14292..15668
                     /locus_tag="PC407_001143"
     CDS             14292..15668
                     /locus_tag="PC407_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HDF2496601.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDASVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            15669..16640
                     /locus_tag="PC407_001144"
     CDS             15669..16640
                     /locus_tag="PC407_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2496602.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYVSSFVSMLVFTLIHVLCRMILINLII"
     gene            16672..17742
                     /locus_tag="PC407_001145"
     CDS             16672..17742
                     /locus_tag="PC407_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2496603.1"
                     /translation="MKDLGHGANVGEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIGINPDFIIPGNGATEVIYLLMKSVKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLGKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRAYKKTTKELKKDLLKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            17744..18631
                     /locus_tag="PC407_001146"
     CDS             17744..18631
                     /locus_tag="PC407_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2496604.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSEVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVIILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDTLCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            18619..19251
                     /locus_tag="PC407_001147"
     CDS             18619..19251
                     /locus_tag="PC407_001147"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HDF2496605.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            19260..20486
                     /locus_tag="PC407_001148"
     CDS             19260..20486
                     /locus_tag="PC407_001148"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HDF2496606.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCKERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            20483..21091
                     /locus_tag="PC407_001149"
     CDS             20483..21091
                     /locus_tag="PC407_001149"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HDF2496607.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNVVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKIPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            21081..21656
                     /locus_tag="PC407_001150"
     CDS             21081..21656
                     /locus_tag="PC407_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HDF2496608.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNIKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            21649..22401
                     /locus_tag="PC407_001151"
     CDS             21649..22401
                     /locus_tag="PC407_001151"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HDF2496609.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            22385..23506
                     /gene="cbiG"
                     /locus_tag="PC407_001152"
     CDS             22385..23506
                     /gene="cbiG"
                     /locus_tag="PC407_001152"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HDF2496610.1"
                     /translation="MNIEGNIAIICITENGKNLAVKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIIFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSIDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPIAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            23503..24228
                     /gene="cobJ"
                     /locus_tag="PC407_001153"
     CDS             23503..24228
                     /gene="cobJ"
                     /locus_tag="PC407_001153"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HDF2496611.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            24225..24977
                     /gene="cobK"
                     /locus_tag="PC407_001154"
     CDS             24225..24977
                     /gene="cobK"
                     /locus_tag="PC407_001154"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HDF2496612.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            24996..26474
                     /locus_tag="PC407_001155"
     CDS             24996..26474
                     /locus_tag="PC407_001155"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HDF2496613.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEEDGFDVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            26504..27409
                     /gene="hemC"
                     /locus_tag="PC407_001156"
     CDS             26504..27409
                     /gene="hemC"
                     /locus_tag="PC407_001156"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDF2496614.1"
                     /translation="MNIVVGTRGSTLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            27399..28898
                     /gene="cobA"
                     /locus_tag="PC407_001157"
     CDS             27399..28898
                     /gene="cobA"
                     /locus_tag="PC407_001157"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDF2496615.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEDIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDNDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEDKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            28915..29880
                     /gene="hemB"
                     /locus_tag="PC407_001158"
     CDS             28915..29880
                     /gene="hemB"
                     /locus_tag="PC407_001158"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDF2496616.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     NSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            30033..30611
                     /locus_tag="PC407_001159"
     CDS             30033..30611
                     /locus_tag="PC407_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HDF2496617.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        12414 a         3064 c         5669 g         9522 t
ORIGIN      
        1 attttggaat caaaatatac taggatataa aattcttaaa taaaaagaaa aggttgtttt
       61 tataaaaaat attttttata ccactttaaa aaacacaata ataacactga gggaggtggg
      121 gatatgagag aacgcattat tcttgaaaca ttaaaaaata gtagtggtta tctaaacata
      181 gattattttg cagataagtt gggggtatct actcgaacta tcagaaatga aataaaaaag
      241 atagaaacta taaaagaatg taatggtttt aaattagaat ataagtctaa attaggatat
      301 attctaaata ttaaagacca agataaattc gagcattatc taagaaactt accctcagat
      361 ttagttgaaa atccagaaca acggttggag tctataatag tagagctttt agtaaatgaa
      421 ggttataaaa ctatagaaca gttaagtaaa aaatttctag taagttcatc ccaaataaaa
      481 aatgatttaa aaaagataga tgaaaagata aaggatacag aattaaaact agagcgaaag
      541 gctcattatg gaattaaaat agaaggttct attaaagaaa ttcaaagtat attggtggat
      601 agttatttta gagggaatag aaatataaca gagtacagga ataagtttat agataattta
      661 aaattagcta atattagaag tacaataaaa aatgtactta atgaacatga tttagaagca
      721 aatttaacag aacttgagga aatattagca caaattatta tattgtatat aagagttagt
      781 atgagagtag tgggaaatgc tagcaattta aaattatata cagaagattt aattatagat
      841 aaattattag ataaaatttt tagagataaa aaatataacc ttaattatga tgaaaagtat
      901 tatttaaagg agcttataaa actaaagact aaagataaga aagccactat aaagaatatt
      961 gataagaata aacttcaaga tattatgttc gaatttttta gagagataga taaaaaatat
     1021 aactctaatt ttttagaaga taaagagttt tttaacttat tttacttaca tattgcatgt
     1081 ttaatagaac gtataaagaa gaaccacaag ataataaatc ctttttcagt taaaataagt
     1141 caacagtatc caactgtttt taatttatct attcaatttt caaaaatcat agaaaatgag
     1201 tatcatataa aaataagtca agatgaaata gggtttatag ctactcacat tgctgttcct
     1261 tttgaaaaga gagaagaagc taattttaat aaaaaatata aaatagcaat aatatgctct
     1321 tctggtggag gtagtgcatt tttgataaat ttaagactta aagaaatttt tccaaatgct
     1381 gagataagaa atttttcatt actagaagaa aaagctgtaa tagaatttgc accagatttg
     1441 attttttcaa taacaaatct tttatttgaa ataaatgctc cagttatact tataaatgaa
     1501 attttagacg aattggatta tttagatatt aaagaaagtg ttagatttgc agaccatata
     1561 ggaaatatat caagcccaaa acaatatata ttaggtctat ttgataagaa tcactttagg
     1621 tgtataaaag ataaagctaa gtataaggat atccttgata gtatgtcaaa atgtatagtt
     1681 gacgagggag cttgttctcc aacctatccc aaagatgtat gggaaagaga aagctatttg
     1741 agtactattt atacaaatgg ggtggctata cctcatccaa tcgagatgac aggaaacaaa
     1801 aatataatat cagtagcttt gatacaaact gatattattt atgaaaatag ggtgcccaaa
     1861 attattttca tgatttcatt aataaaggga aatttagaac ttcataagca aatatctaaa
     1921 tatttaagta agattatggc aaataaggat atggtagaca tgctaaataa gtcacaatct
     1981 tatgaagagt ttatgtataa gttaaaaatc tatctgggag gttagacatg aatatagagt
     2041 tttttaagag gttgtcagaa gcagatggta tagcatctaa tgaagaagag gttagacatg
     2101 ttttattgga agaactaaag gcttatagtg ataaaattat ctgtgatggg ctagggagta
     2161 tcatcttttc taaaataaaa gatgagagtg caccaaacgt aatgatttgt gctcatatgg
     2221 atgaagttgg atttatggtg agaagtatag acaaacttgg tatgattcac ttaataacaa
     2281 taggaggagt aaaacctctt gcacagtttg tacaaaaagt tagaattaca acaaaagaag
     2341 gaaagaaaat ccctgctgtc ataaatgcta catataataa tggtaaggct gaaaatatat
     2401 atgcagatat tggagcatat acagaagaag atgtatataa cctagggatt aatgttgggg
     2461 atatggtaac atatacaaca agttttgagg aatttacttt accagaccga ttagtgggaa
     2521 aggcttttga tgataggata ggatgttttg tgatgggaga agttttaaaa gaacttagga
     2581 aagaaaatct aaattgtaat attcactttg cggctacaag tagtgaagag gttggaataa
     2641 ggggagcaaa gacatcaaca cagttaataa atccagatat agtatttgta atagatgtag
     2701 catgtgctaa aaatgagttt gtgagagact atacaaatca gaaacaaatt gataaaggtg
     2761 taatgctaat gcatagagat aggactctag tccctagtag aaaaatgata gattatgtaa
     2821 tggaaattgc tgataaaaat aatataccac ttcaacatga tatgtttgaa tcaggtggga
     2881 ctgatggagg agaagcacat ctcgtaaatg aagggaaacc ttgtgtagta acttgtgtac
     2941 cagttagata tggtcattgt gcattttcaa tagtaagtaa taaagattta gagaacatta
     3001 taagattgta tacacagtta attttaaatt ttgatgaaga acaatataaa catataagaa
     3061 attttatcta aaggagaata agaaaatgtc aacagaagta caaataatgg agcttatatc
     3121 aactgcagga gagagtaaat cgaaagcttt tgaggcttta aaaaaagtaa agtcaaaaga
     3181 ttttaaagga gcaaaattgt taatagagga ggctcgtaaa atagatattg aggctcacag
     3241 aatacagaca gaattaataa ctcaagattt aaatgaagat gttgaaagcc caacaatgag
     3301 tcttttaatg gtacatgctc aagaccacta tatgacttct cagctagcaa aagacttgat
     3361 agaggctttg atatattgtc tcgataagta attgtaatgt agaaatagat tatttatgta
     3421 aaaattcttt taaaagttta gatgctttta agagataata aaaatacaaa ctttaggagg
     3481 aattattatg aaaatattat tatgttgtgc aggtggattt tctactaata tgttaatgca
     3541 aaatatgaaa aaggtggtaa aagagagtgc taaactcaat gaaaaagatt ttgagtttac
     3601 agctatacca gcagattcaa tagaaagtga agttgatgat tgggatatag tacttatagg
     3661 tcctcaaata gctcataaaa tagacttctt aaaaggaata ttagagccaa aaaataagcc
     3721 ttttgcagta atagataaag atgtatatgg aaaaatggat ggagctacag ttatgaagtt
     3781 ggcattaata acttataaga aacatcaatt agctcaagga taataaaaat aatgagggaa
     3841 aagggagatt agatatgttt tcaaaatttg aagcatttat gaatagagta tttatgcctt
     3901 tagctcataa ggttgacaat caacgtcacc ttggttctat aaaggctggt atggttgcta
     3961 tgacaccatt cacaatatta ggaagtatat ttgcaatact tccagcactt ccaaatatgc
     4021 ttggagaaaa taatccaata agcaaattca tacttaacaa ttcagcaata ttggatttac
     4081 cagtaagtct ttccataggt ttaataggat tatatgcttg tatgtgtatt gcttttaatc
     4141 tggggaacta ctataaatta tatataccag gttgtgttgc actatcaact ttttcttttt
     4201 tattcctagt agcctcattt acaccagatg gagctttaga aataggaaat ttaggagcta
     4261 aagggctatt tacaggtatg atagcagggc ttggttctgt ggagttatat aatttttgta
     4321 ataagaagaa tatcacaata aaaatgccag aaaatgtacc tgattttgta tctaggtctt
     4381 ttgaattaat acctactact ataatagttt gtggaacttt tatagttgct cgttttattt
     4441 cattaaaagt atttggagaa ttacctccac agatacttac tcaattttta tctccattag
     4501 taggaagtat ggataaccca tggtcagttc ttgcacttaa ctttgctata tgtactatat
     4561 tcttttttgg aatacattca tcagtattta gtcctataac tagacctata atgattacat
     4621 ttatagctga aaacatagca gctatgcaag ctggagaaac aatacctcat ttctttacag
     4681 caggagcttc aagtgcattc tttggattta caggatgtgg aattagtata ggttgtgtaa
     4741 ttgcatgtat gttatcaaaa aatgcttcct ataagaagat tggacgtgta tcattattcc
     4801 ctgcattatt tgggataaat gaaccaatat tatttggagc accgataata ttaaatccaa
     4861 taatgtttat accacatgta tttggtggag ctataatagg tacactacca atgtttttta
     4921 tgcactgggg atggttagca aagccagtat ttgaccctcc atatgtaggg gtattcttag
     4981 aaggattttt aactaatgga gattggcatt ctatattagc aaatgcattg caattagtat
     5041 tagcagtttt attatattgg ccattcttta aaataatgga acgtcaacaa aataataaaa
     5101 aacaaaaaga agaaactaag aatatattct ctaaagaaga agaagactta ttggatgatt
     5161 taggattgga tttctaatat tttgttatga ataatacaat agagagatta gtatccttaa
     5221 tctataaaag aggtgttgac gctcttttga ttaaagcaaa aaatactaaa agatatatag
     5281 gagctttatc aggtagtggt gtatatgtgc ttgttactaa ggataaaaat tatcaaattc
     5341 tagatggcag atatattgat gaagcagata aaaaaacgac tggtttcata aaaaggattg
     5401 tatctcaagg ttcttatata ccaactataa ttgagttatt aaaagaatta aatataaaaa
     5461 agttagcaat agaaaaccaa gctatgagta tacaagagta tctttcacta aaaaatgaag
     5521 gttttgaaat caatttaata acaaatgaat tatggaagct aagggctata aaatctaaag
     5581 aagaaattga acttattaaa aaggcttgtg agattacaga tgaagtattt gatgaggtta
     5641 tttctgaaat taaaaaagat atgactgagc tagaagtatc agctctaatt cagtatcatg
     5701 cattaaaaaa aggtgcaagt ggaatgtctt ttgaaacaat agtggtatca ggagaaagag
     5761 gtgcaatgcc acatggaaga cctacaaaca aaaaattgaa agtgaatgaa gctataacaa
     5821 tagattttgg tgttgtttat caagggtatc aatcagacat gactcgtaca atatctatag
     5881 gtaaacctcc taaaattatt aaagagatat atgatgtagt attggaagca caattatctg
     5941 caatagagtc aataaaggaa ggaactaggg caagtgatgt tgacaaagtg gctagaaaaa
     6001 ttatagataa acatggattt ggcgaatatt ttaatcatgg actgggtcat ggtataggtc
     6061 ttggagatgg tgaagttccc actttaaatc caaatagtga agatatatta gtggaaggaa
     6121 tggtaatgtc ttgtgagcca ggtatttata ttccaaatgt agggggagtt agaatagaag
     6181 atgatattgt tataatagat ggaaaaggtg ttccactcaa taagacatcc aaagagttta
     6241 taatattagg agagtaaaat tatgaaatat aattttaata aagtattaaa tcgtaaagaa
     6301 ggaaattgta gaaaatggag caaccatgtg attaaagaaa aattcggctt aaatgaagat
     6361 gcaattccta tggatttagc tgatatcgat tttgagtgtg ctcctgctat aaaagaatct
     6421 atcttaaaaa gagcatcagt aggagattat agttatactt ttataggaga tgacttctat
     6481 gatgctatta taaattggaa taaaagaaga tttgatgtat atatagaaaa ggattggata
     6541 aaattaactt ttggtactgt ttcaaccctt cattatatag ttcaagcata tactaaagaa
     6601 ggagagggtg tattgataaa tacacctgct tatgaccctt ttgcagaggc tgtagcaaat
     6661 aataatagaa agttgtgttg tagctcatta aaattagata acaatagata ttatttagat
     6721 ttagaggata tggaaaatca aatgaaatac gagaatataa gattacttat attctgtagt
     6781 ccacaaaatc catcaggtcg tatatggaca aaagaagaac tatatcaagt gtcagagtta
     6841 tgtttaaagt ataatgtaat tttggtaagt gatgaaattc atagagatat aatatttaaa
     6901 gattatagat ttgtatcttt gtggaatgct catccagaaa tttataaaaa tagtataatc
     6961 tgcgtatctc ctaataaagg cttcaactta ggtggattaa aaacatcata tgtattaata
     7021 agagatgtaa aaattaggga gactttatta gaaaggctaa aaagtaattc aataacttct
     7081 ccaaatgtat ttgcaatacc agcaatagtt gcagcataca atgaaagcga agaatggcta
     7141 gatgctatga ctagttatgt agaggagaat tttgaaatag tttatgattt ctttgaaact
     7201 aatataccaa aagccaaagt gatgaagtca gactcttctt ttttagcctg gatagatgta
     7261 agagaagtat ttagaaatga agaagaatct aaagaatttt ttagacatgc aaacttaact
     7321 atggtagttg gaagttattt tgtgaaagat ggagatggat ttattcgtat taatgtgggg
     7381 tgtcctagag aaacattaaa tgaggctcta aatagaatca aaaaaacgta tataagtatg
     7441 tattgttaaa tcataaaaaa agtatctatg gtttatataa aaacacatta tttagttatc
     7501 aaatattact ggaattttgt attccagtaa tattttttga ttaaggaata ttattgagga
     7561 atattaaata atattatttc atacccttat attaacttat ataaaaatat tttattattt
     7621 tgggaacggg ttagctaacc tttatctagt gtatatttat attgtaagaa gcagtaaaaa
     7681 cttttccctt actaagttta ttaataaact tcttatcttt ataaattttg ttagaattta
     7741 tactatcagc aagaccatac caaggttcta tacataaaaa aggagcagta gaattagttt
     7801 cactataata aggagtccaa ataccaacta gagggaaatc ttccatagat atagttacat
     7861 atttactacc atcattatta tatatagaca cttcatctat gttggtgtat atcaaagcat
     7921 cattcttgaa tacttctgga tttaaatgta agttttttaa ttttccaata ggtattatat
     7981 catctgtaaa agaaccattt aaattgattt tttctacatc atttttactt ttaaactcaa
     8041 ggtagtgttt agaaaaatca ttttgtttgt aaaaaggaag gttgaatgca ggatgtccac
     8101 ctatagaaaa gaatatatct ttagagtcag tattttttac aatccattct acctcaatac
     8161 tattatcatt taatgtatag tttatgatta gctcaaaaga atatggatac ttctttaaag
     8221 tggattcatt atctgttaat ttataagata caaatgtatc tcctttatct acaatctcaa
     8281 agtccatatc tctagcaaac ccatgttgag tcatgctata taaattttct tctattattg
     8341 tttcattatc aaaaagttta cctactatag ggaacaatat aggtgattgt ctaccccaat
     8401 attttttatt accatcccaa agaatgtttt tgtcgtattt tttagaaaat attttagtta
     8461 gttcagcacc aaaactgctt gattcgataa ttaaattttc attttgtaat atattcattt
     8521 aaaatctcct ttattatatt ttatgagtat attatactat aaagtataaa gattattaaa
     8581 ttttatttga tattatattt gaaaggtaga ttttagtata ttttgaaaat tttgttttga
     8641 aaatttggga ttatttaaga aattttgtat tataaataga aggaaataga taaattgtat
     8701 agaataaaat atattaagat aagttaataa tgttaaatgg gaagtgagtt tatggaaaaa
     8761 ccaaatacaa aagttgttaa ctttcaagag tataaagaca gaaaaaacca aagagaagta
     8821 aatagagacg aacttttaaa gcttataaaa aaaattgtga cttcaaaata gggagttgtt
     8881 aaactcccta ttttaatggg tttcataata attaagacat tctgagtcca tctatataat
     8941 ctttgaattg tgagaaatct gattcactac ttaattctat ggtttctgaa ccttccgtat
     9001 aaaaattctt aagatagata gtataattat tatcaacaca atcatgataa atacatctat
     9061 atccatcttc atacaaactt tttaacttag aaaaatcatc cacaatatca tctccttaaa
     9121 gttcttttaa atatattttt acaaaaagca tctaattgta tgcaaattga caatgtaaaa
     9181 taaaggaaat gaacataaaa tgtagaataa tttttatatg tatgtaaaaa taaaaagaaa
     9241 aagttgaaat ttttagaaaa taagagtatt ttttatctaa aaagagagtt taaaaaataa
     9301 aaaaatgttg ggaggataaa aaatgagttt attagaaagt atatcaaaaa acatatattc
     9361 cctagataac tcttctattg aaaaaacaaa gcagagattg gataggctta tacatccaac
     9421 tggaagctta ggaaagatag aagatatttg catgcaattg gcaggaatat ttggcaatga
     9481 aaattttgac acaagtaaaa aggttataat tgcttttgcg ggagaccatg gtgtatatga
     9541 agaaggcgta gcacctgacc ctcaaaatat aacaaaatta cagtttccaa atttttcgaa
     9601 aggtctatgt ggagttggag taataagtaa atttgtaggg gcagatgttg tagctgtaga
     9661 tgtaggaata aactgtgatg aaaaactgga tggggtatta gactacaaga taagaaaagg
     9721 gacatcaaat atggcaaaag gtccagctat gagtaaacaa gaagccatta gatgtttaga
     9781 gataggtatt gaaatagcag agcagtgtat agaaaaagat tataaggtta ttggtattgg
     9841 ggagatgggt atagcaaata ctactccaag tacagcaata atatctgtaa tttcagggtg
     9901 tgacccgtta gaagttactg gtataggtgc aggtcttaag aaagaaagac taaagcataa
     9961 ggctgaagta attagaaagg ctattgaaat taataatcca aatccaactg atggagtaga
    10021 tattttatct aaagtaggag gatttgaaat aggctcaatg gcaggtgtta tattaggttg
    10081 tagtgctaat aggattccag tagtaattga tgggtttatt tcttatgcag cagcattaat
    10141 tgcatataag ataaatccaa aaactagaga atatatgata gcatctcatt tatcagcaga
    10201 atcaggaact aaaagagcat tagacatatt aaaattagac cctctattaa atatggatat
    10261 gagattaggt gagggaagtg gagctgcttt agcatttgat ataatagaag cttcaaacta
    10321 tacttacaaa aatatggcaa cttttgatga aattgacatg ggaagataaa tataaaaatt
    10381 taatatgtag gagtgttttg atgagtaaaa ttattctagt aacaggagga gctaggtcag
    10441 ggaagagtaa ctttgcagaa aaactatgtt ttgacagaaa taataataca gcttatatag
    10501 caacctctat tccatttgat gatgaaatga aagatagagt aaaaaaacat caagagaata
    10561 gacctagaaa ctggaatact tatgaaatat atgaagatat atattccata attaaggaga
    10621 tatataaaga acatgaaact gttatactag attgtgtaac attattagta aataatctaa
    10681 tgtttgaaca caatctaaat atagaagagt ctagtcaaga agaaataaac aaattagaac
    10741 aatacataaa agaacaagtt gtcaaactta ttgaagaaat agaaaaaaca aatctatact
    10801 ttgtatttgt aacgaatgag ttaggtatgg gaattgttcc tgggaataaa cttagtagaa
    10861 tttatgctga tattgtaggt cgtataaatc aatatatagc ttcgaaaagt aatgaagttt
    10921 attttgtagt tagtggcata gctatgaaga taaaggagta gtatgcaatt atgaaaagat
    10981 ttattttaat actacagttt ttgacaagga tacctataaa attaaatgta ggatttgatg
    11041 atgaatttta taagtcaata gtatattttc cacttgtagg ttttgtcata ggtatattat
    11101 cctatttaat aggatggatt tctatgcttt tatttgaacc atttatagct tctataataa
    11161 taactctagc tggtgtctta attactggag gacttcatat agatggatta ggagatacat
    11221 ttgatgcaat atatagttat agggataaag aaaaaatgct tgaaataatg aaagattcaa
    11281 gattaggtac aaattcactt ctagcaatta tgtttgtact tttattaaag gttggattcg
    11341 tttacgatat aataagtaat aattcattgt ggattataat ttttatgcca atgattgcaa
    11401 ggttaggagt aatgttattg acatataaaa ccgtgacacc aagagaaaaa ggaatgggaa
    11461 atttatttat aggaaaatta actacaagta tgttaataac tgctataata tacacattat
    11521 tgatagttgc tcttattact aaatttatat ttttattacc taatatagtg ttgattaaag
    11581 tattaggttc gattatagtg gtttttgtat ttataatatt attcaaaaaa catatttata
    11641 aaaaaattga cggagtaaca ggagatatat taggctgtgg aatagaactt tcagagcttg
    11701 tatatttaat ttacatatat ttgttaattt ttatgttttt ttaaagtaaa aagtggtgat
    11761 aatatgataa gattaatatt aataagacat gcactgacaa atgataataa aaaaggaaga
    11821 ttatcaggcc atataaatag ttgtattagt gaagagggca aactacaaat aaataaaatt
    11881 accagatatc tgagtaatga aaacatagat aaggtttata caacaccatc tacacgtact
    11941 aaagatacag tagaaaaaat atcaaaattg aaattattag aaatagaaga aaaagaagct
    12001 ttaagagaaa ttagttttgg agactttgaa ggaagaactt ttgaagaaat taaaatcaaa
    12061 tatccaaatg agtttgaaaa aatgataaaa gaaggtaaca actatagata tccaaatgga
    12121 gaaagtttga tagactctta taaaagagtg gcaaaagaaa ttgataatat aattttagaa
    12181 aatgatagta atttagatac aaagacaata cttatatgtt ctcatgcagg aacaataaga
    12241 aatataataa cacatttgat ttctggtagt tataagtatc actggaactt taaaattgat
    12301 aatgcatcta ttacagtttt agaaattgat ggtggatttg ctgtaataga taaaatgaat
    12361 tttacagact tcatataatt taatcaaaac caaacattac aatctgtgtt tgtaatatat
    12421 ctgtaaaatt agcaatgaaa atagaatatt agattaaaat taggttctta attagatgtt
    12481 aaaagggaaa aaggttagat gcctttgcag cccccgctac tgtgaaacca acgaaactat
    12541 aaataccact gtcagtttga tgggaaggtt atagaagtag gatgaagtta agccaggata
    12601 cctgcctaat ttaatttaaa acaagaggtc ttcggggtga aagattttta attaatatat
    12661 ataaaagtcc tatctttaga agtattagga ctttttttaa tttgtttttt agtaaatttt
    12721 aacaaggagg gatggaaagt ggggaaaaaa ataatgctac aaggaacagc ttcaaatgta
    12781 ggtaaaagtg tcttagtagc aggtttgtgt agaatattta agcaagatgg ttatactgtt
    12841 gctcctttta agtcacaaaa tatggcttta aattctttta taactaaaga aggattagaa
    12901 atgggaagag cacaagtatt tcaagcagag gctgcgaaaa tagagccaat agctgatatg
    12961 aatccagttt tattaaaacc ttctgggaat aataaaagtc aagttattgt aagaggtaaa
    13021 gttgtaggag aaatgccttc ttctaagtat catgattata aattggagtt agtaggtgtg
    13081 ttgaaggaaa catttaatag cttaaattca aggtatgata ttgtagttat ggaaggggca
    13141 ggaagtaatg cagagataaa cttaaaagat agagatatct ctaatatggg tatggcagaa
    13201 atagctgatg cacctgtaat aatagtcgga gatattgata gaggaggcgt atttgcctca
    13261 ttagcaggaa caatgctttt attaaatgaa gatgagaaaa gaagagtaaa aggtgtaata
    13321 atcaataaat tcagaggaaa aaaagagctt ttacaagatg gtgtaaagat gctagaagac
    13381 atcataaaag tacctgttat aggagtagta ccttatacag atataaaaat agaagatgaa
    13441 gacagtgtaa ctacgagatt taagaaaaaa atgaataaaa gtgatataca catagaaata
    13501 attagaaccc cacatatgtc aaattttaca gattttaata tatttgagac acaagaagat
    13561 gtaagtatta gatatgtaga ctatggagaa tctttaggaa atccagatat attaataata
    13621 cctggaacta aaagtactat agacgattta acatatataa gaaaaagtgg tcttgaatcg
    13681 caaatcaaaa atttacatag tcaaggtaaa ttgatatttg ggatatgtgg tggataccaa
    13741 atgttaggta aaaaattaaa agacccttat catgttgaaa gtgaaattga agaagttgat
    13801 ggaataggtc ttttagatac agaaacaacg tttgagaaag aaaagactac aacacaagta
    13861 gaggctacta ttgttgaatg tatcagtgaa tacatgaata accttaaggg aaaaaaagta
    13921 aaaggttatg aaattcatat gggcattaca aagcgaggta atgatgtaag aagtttagat
    13981 ttaataacta aaaaacttga taaagaagtg aattatactg aaggaagtgt aaataaagag
    14041 gggaatgtaa ttgggactta tcttcatgga atatttgatg aaataagctt tacaagagca
    14101 attttaaaca atataagaaa gaaaaaaggt ttaggagaat tagaaagcaa agtaaactct
    14161 tttaatgaat ttaaggataa agagtatgat aagttggctg attttttaag agaacattta
    14221 gatatagaga aaatatatga gattatgaaa taatgatgac ctttatttat aatttaggta
    14281 ggaggtgtat tatgaaaaaa attttaattg ctggtactaa tagtggagta ggtaaaacaa
    14341 caatatcatt aggaataatg caagctttgg ttaagagaaa tatgaaagta cagccatata
    14401 aagtagggcc agactatata gacccttcat atcacacttt tataactgga agacattcta
    14461 gaaatttaga ttcttatatg ttagatgatg aaaaaataaa gtatatagta aataagtcct
    14521 caaaagatgc agatatttca gtaatagaag gtgttatggg cttatatgat ggttttggaa
    14581 tagatttaga taattgtaca agttcacata cttcaaaggt attaaagtca cctgttatat
    14641 tagttataaa tggtaaatct atggcagcat ctagtgctgc tatggtactt ggatataagg
    14701 aattagataa agatgtaaat ataaagggtg ttatagtaaa taatgtaaag actaatagcc
    14761 attatcaaat aattaagagt tctatagaaa aatattgtaa tgttgaagta ttggggtatt
    14821 ttcctccaaa taacaaattc tctttagaat ctcgacatct aggattagtt cctagtgtag
    14881 aaatgaaatc actaagggaa aaattttata ccttagcaga tgaaattgaa cactatatag
    14941 atattgacag aataattgaa atatcagaaa gcgatgaatt tgagagtgat tttgattttc
    15001 aaactttgat agaaaataat aaaataaaag aacatgttaa taataaatca atagctatag
    15061 catatgataa agcattcaat ttttattata gagaaaatat agagctttta gaggaacttg
    15121 gtttagaaat aaactatttt agccctcttg aagatgcatc agtgccaaat gcagattata
    15181 tttatattgg aggaggtttt ccagaaatat ttggaaaaga attagaagca aatgagtcaa
    15241 tgagaacttc catattagaa gcacataaca ataatatacc tatttatgca gaatgtggag
    15301 gactgatgta cctaggtgag aaactattga atcaagaaga acaagaattt aatatggttg
    15361 gaatatttag tggaattagt aagatgacat cttctttaaa gagatttggg tactgtattg
    15421 gtgtagccaa ggaaaatact cttttatcta aaaaagggga atctattaaa gggcatgagt
    15481 ttcatcattc aatatttgaa agtgatgaaa aatgtgtata ttcaatgaga aaagaaagag
    15541 atggaaatat agtagaagag tgggatggtg gttatagtaa aggaaacact ttggctacat
    15601 atcttcatac tcatttttat aataacttaa attgtataga aaattttttg aaaaaaggaa
    15661 atgaataaat gagtatttta tctatttata taggatatgt gacagactta attgtaggag
    15721 acccatattc ttttcctcat ccagtgagat ttattggtaa acttataaat tttactcaag
    15781 gtataataag aaaggtctct aaaaatgata ggcagttaaa atttggggga tttatattat
    15841 ggtttataac cgttggaata acatatttaa caacttatgc tatagtgaag ctatttagtt
    15901 ttaatatatt attgagtatt gtagtgaata gttttataat ttatacaaca ctagccacaa
    15961 aatgtttaaa agacgaagca ttaaaaattt ataatgtatt aaaaaccgaa gatatagaaa
    16021 aatctaggat tcagctatct tatatagtag gtagggatac tactaattta agtgaacctg
    16081 aaataataag agcaactgta gaaactgttg cagaaaatac tgtagatggt ataattgcgc
    16141 ctatgtttta tgcatttatt ggaggagcac cacttgctat ggcctataaa gctataaata
    16201 cattagactc aacagttgga tataaaaata aaaagtataa agatattggt tttgcatctg
    16261 ctaagattga tgatatagcc aattatatac cagctagaat atctgttatt cttatgacaa
    16321 ttggaagttt ctttttaaat tataactata ggaactgttt taaaatctct attagagata
    16381 gaaagaatca taaaagtcca aattgtgctt tttcagaagg cgcagtatct ggtgctctag
    16441 gaatacaact aggaggtacc aacgtttatt ttggggaaaa agtatataag ccaacaatag
    16501 gggataagct tagggagata gataaagaag atataattaa aactaataaa attatgtatg
    16561 tatcttcatt tgtatctatg ttggtattta cactaataca tgtgttatgt aggatgattt
    16621 taattaactt aattatataa attattgttt gctaacttaa gggggacata gatgaaggat
    16681 ttagggcatg gtgccaatgt aggtgaaatg gcaagattat atgggaaaaa cccaaaagaa
    16741 ataattgatt ttagttcaaa cataaatcca aatgtattac caaatttaga gagatacatt
    16801 ttaaaaggat tagaagagtg taggaattat ccagatataa attatacaaa cttgagagaa
    16861 aatatatcaa aatatatagg tataaatcca gactttataa tacctggaaa tggtgcaaca
    16921 gaagtgattt atttgcttat gaagagtgtt aaaaaaaaat tggctataat aaatccgact
    16981 ttttcagagt atagaagaag tgctgagtta aataatttag atattataga tttagagcta
    17041 gatttagaaa ataactttaa acttaatata gatataataa aagaaaatat aaaaagattt
    17101 gatagtttat ttatatgtaa tcctaataat cctagtggta atgtccaaga tttaaaggaa
    17161 cttgttcatt tactaggtaa acataataag gtactaataa ttgatgaaac tttcatggaa
    17221 tttgttgaag atgaaagtaa atacagtttg gttaaatata tagaatcaaa taaaaatata
    17281 tttataatta aagctgtgac taagtttttt ggtatgcctg gtttaaggct aggatatggt
    17341 cttacaagca acactgaaat tatgaacaaa atttatgaac acaaagagcc atggactatt
    17401 aattcttttg cagatatact atccaatttt atttttgaag ataaagagta tattaaaaat
    17461 agtaaagaat attacataga agaaagaaaa tatatgttac aagagttgag aaacataaga
    17521 aatataaagg tctatgatac agatgctaat tttattttga taagagcata taaaaaaaca
    17581 actaaagaat tgaaaaaaga tttgcttaag caaggaaaca tattagttag agatgcgtct
    17641 aattttatag gattagatga tagttttata agagtggcta taaaatctca tgaagataac
    17701 aaaattctta tagaaaatat gaaaaattta ttgggtgatt aaaatgaaat cttatggaat
    17761 atgcccagca tcttgtggag aatttgtaca aggtataatt gatgatgaag aatatttatg
    17821 ttcatatgca atagatatgt attctgaagt gtatattgaa gaaaaattag tagatataaa
    17881 tttagggaga tataagtcaa ggttagcaat tgaaaaggta tttgaaaaat ttaatttacc
    17941 taaaaaatat actaaaaata tttctttaaa tataaatagt aaaatacctg ttggaaaagg
    18001 aatggcaagt tcaactgctg atataggagc tacaataaag gcaacattat cattgataga
    18061 taaagattta agtagtgagg agatttctaa attagcagca gaaatagaac caacagattc
    18121 tatatttata gataaaaata gtatatttaa tcctctaaat ggaactgtta taaaatacct
    18181 aggaaatctg accaatgcga aagttataat acttgaacca aacaaagtac tagatactat
    18241 gaaaattaga ttgaggcaag attataataa attaaaagta gaaaataaag aagtaataaa
    18301 aaaatcattt gctcttttag aagaaggatt gaaaaaaaat aatttatctc tagttggaga
    18361 ggcttgtact ttgagtagct tagcaaatga gaatatagaa aaaaaagagt atttaaatga
    18421 aataataaaa atatcaaaaa aatatggagc atatggagta aatattgctc acagtggaac
    18481 tgtagtaggt attttaattg ataagtctat gaatgataaa aagatgatag ataccttatg
    18541 tgaatctaac ataaattctg tctataataa aatttataca caaaacataa ttaatggagg
    18601 aattaagggg gaaatagaat ggaatacata aagaatccta tgaaaataga agaaaaaagt
    18661 tttgaattga tacaagaaat aatagacgaa attagaccag attataaatt taagaataat
    18721 attgaagaaa aaataataaa aagagctata catactactg ctgattttga ttacttagat
    18781 atattaaaaa tatcagaaga agcagttgat agtataatag atgcattaaa aaataatgca
    18841 agtatttata ctgatacaaa tatggctctt agtggtataa ataaaagaaa gttagaatca
    18901 ttaggatgta agtataaatg tttagtggca gatgatgaaa ctgtaaaatt agctaaagaa
    18961 aaaggaatta ctcgttctat ggcagcagta gaaattgcag ctaaagaaga aggaagaaaa
    19021 atattcgttt taggtaatgc tccaacagct ttatataaag ttatggagat gaaatcagaa
    19081 ggtagattag atttagatgc agttatagga gttcctgtag gttttgtagg agcacaagaa
    19141 tcaaaagatg aagttgaaaa aacagatata ccatatataa tttctaaagg tagaaaaggt
    19201 ggcagtaatt tagcagcagc aatagtaaat gctatattat atactatgta ggtacctata
    19261 tggaagaata tgtatatata gatggaaaaa aatatagaag aggatatact acaggttcat
    19321 gtgcaacagg tgcatctaag gctgctgtat atatgttgat tacaaaaaat agaatcaata
    19381 ctataaatat agatactcct aaggggatac cattactact aaaagttgat aatataaata
    19441 tttctgatac atttgtggaa tgttccataa aaaaagatgg tggagatgat atagatgcaa
    19501 ctcataccat ggatatttat gcaagagctg aaattgtagc taaaaatgat aagaacaaag
    19561 ggtatcttac tctaaaggat atagatagtt taagcaccaa tagtgagtgt aaaagtgagc
    19621 tctataagtt tattagggtt tatggcggaa ctggtattgg tgtggttacg aaaaaagggc
    19681 ttagtgtgga tgttggtaaa cctgctataa atccgactcc acttaaaatg ataaaccatg
    19741 aaataagaaa gcttatagga gataattttg aatctatact tggaaatgat aaagttttaa
    19801 aaataacaat ctttgctcca caaggagaaa ctgtagcaaa aaaaacattt aatccaagat
    19861 taggtatagt tggaggaata tctataatag gtacaactgg gattgttgaa cctatgagtg
    19921 atgagggttg gaagaagtct ctttctatag aacttcagat gaaaaaagaa caaggcttag
    19981 ataaaataat attggttcca ggaaatcatg gagaacagtt tataagagaa aaattaaact
    20041 tagatattaa gtatgtagtt agggtaagta attttatagg ctatatgatt aaagaagctc
    20101 aaagaatagg atataaaaaa atcttaatgg caggtcatat aggtaaattt ataaaagtat
    20161 ctgctggtat ttttaatact catagtaagg tagcagatgc tagaagtgag atattagtgg
    20221 caaacttagc tttaatggga gctagatacg aatttttaaa taaaataaat caatgcgtaa
    20281 ctactgaaga agctgtagag ctaataaata atagtgaata tagagaagta tataatatat
    20341 taagtaataa atgtaaggag agagtaaagc aatacttgaa tgaagattca gatgatattg
    20401 atgtggaagt aatcatattt tcaatggata agtctttatt aggtaaatcg gataatacag
    20461 atgatttagt ggaggtcttt atatgataaa cataataggt ctaggacctg gtaacttaga
    20521 ctatataaca aaaaaaggtg aaaatttaat ttccacttct gatgtattaa ttggtggtaa
    20581 aagaaatcta gagtctataa aaaattttga aggagaaaaa atagttctgg attctaattt
    20641 aagagaaatt atagagtaca taaataacaa taaagaaaag caaatatcaa taatagcatc
    20701 tggggaccca ttaatatatg ggataggtag atatctatct aaaaatattg ataataagat
    20761 gttaaacgtg gtttctggga ttagttcatt acagtacata ttttcaaaaa tatatgtaga
    20821 tatgaatgat gtatatatta caagtagtca tggcaaaata ccagattttg attacatatt
    20881 atcacataaa aaagtatgta tggttacaga ttcaaagata ggaccaaaac aaataagtag
    20941 agaaataata gatagaaatc taaataaaat tattgttgta ggagaaaatc tgtcatatga
    21001 caatgaaaaa ataacaatag cgaaaccaga agagataata agaatggaca attttgatat
    21061 gaatgttgtg gtgattttag atgaggaata gtgaatttat aacaggtaaa gttccaatta
    21121 ctaaggaaga ggttagagca atatcaataa gtaagttaaa tttagtaaat gctaaaaacc
    21181 tcatagacat aggggctggt acaggaagtg taagtgttga agctgcatat aattatccta
    21241 atttaaaggt tatatctata gagaaaaatg atgacgctat agagcttata gagaaaaata
    21301 taaaaaaatt caacttaaaa aatatagaag tgataaaagg atatgctcca attaatctag
    21361 ggttaaatgt taaagttgag tctatttttc ttgggggaac aggaaataat ctggaagaaa
    21421 taatagaatg gtctaaaaaa aatctcgtta caggaggaag acttgtagct aattttataa
    21481 taatagatac atttaatcaa actttaaaac tgcttaaaaa gcatggattt aaagaaatag
    21541 atgtatgtgt tttgaatgtt tctaaactag agaaactagg aaaaggtgaa tattttaaac
    21601 cactaaatcc tatatatata atatcttgtg aaaaagggga aaatgatgat gaataaagta
    21661 cattttgtag gagcaggacc tggagataaa gagctgataa cattaaaagg atacaaatta
    21721 ttaagtaatg cagatgtagt gatatatgca ggttcacttg ttaatcctga acttttggaa
    21781 tactgtaaag aagattgcca aatacataat agtgcacata tggatttaca agagataata
    21841 gatgttatga gagaaggtat agaaaataat aaatctgttg taagactgca aacaggtgat
    21901 ttttctatat atggttcaat tagagaacaa gtggaagatt taaataaatt gaatatagat
    21961 tatgactgta ctcctggagt aagttctttt ttaggggcag catcatcttt aggggttgaa
    22021 tatacagttc cagaaatttc tcaaagtgta attataacaa gaatggaagg cagaactccg
    22081 gttccagaga aagaatcaat acaatcatat gctaagcatc agacatctat ggtaatattt
    22141 ttatctgttc aagaaattga gaaagttgtg tctaaattac tggaaggtgg atatccaaaa
    22201 gatactccta tagcagttat atacaaagca acttgggcag atgaaaaaat agttaaagga
    22261 actttaagtg atatagctgt aaaagttaaa gaaaacaaca taaataagac tgctttaata
    22321 atggttggga gatttttagg agaagagtat aataattcaa agttatatga taaggatttc
    22381 aaacatgaat atagagggta atattgctat aatctgtatt acagaaaatg gaaaaaattt
    22441 agctgtaaag ataaattctc tagtaaataa ctgtgttata tatcaagtaa aaaataaaaa
    22501 aagtaattta gttgttgaag aaacatgtgt acatacagtt caaaagaagt taagtgattt
    22561 tgtaggagaa atatttagca aatttgatta cataatattt ataatggcta caggtatagt
    22621 tgtacgaagt atagcaccat acataataag taagtttagt gaccctgcaa ttttggtaat
    22681 ggatgaaaaa ggcaaaaacg taataagttt attgagtggt catattggag gggcaaacag
    22741 cttgacacag tacataagta gtcttattga tgctaatcca gtaataacaa cagctactga
    22801 tataaatcaa aaagcttcct tagatatgat agcaaaaaaa ctaaatgcac atattgataa
    22861 ttttagagaa agtgtaaaag aagtaaattc tatgctagtg aataataaat gtgttggatt
    22921 atatgttgat gaaggatatc aagttgatac taggggattt aaacttatag aaagtaagga
    22981 tgagttaaga gatagctttt caaacacata tatagaaaaa ttagttgtag taagcaataa
    23041 aataaattgt attgaagata ttgagaattt aaacttagat aataaatttg aacattgcta
    23101 tagtactaaa aaaataataa aggtaattcc aagagatata gtgataggaa ttggctgtag
    23161 aagaaacaca gatagtgagc tgatgtataa ttcacttgaa gagtttctta ataaacaaaa
    23221 tatagatatt agagcaataa acacagttgg aagtatagaa ctgaaaaaag acgaaaaagc
    23281 aataattgaa ttatcaaaaa tactaaaagt gccatttaaa acattttcaa tagatgaaat
    23341 atctaaggtt gataatcttt ttgataaatc tgattttgtt aaaaaaaatg tgggcgtgta
    23401 ttcagtagca gaacctatag catatctttt gagcaaaggt aatttgttag ttgaaaaaca
    23461 taaatataaa ggaataactt tttcagtggg gagaataaaa atatgatata cgttgtagga
    23521 attggaccag gaagtaaaga tacaatgact ttagaagcaa taaaagcaat agaagattct
    23581 gaggctattg ttggatataa gacttatata aaactaatag aagaatttat acaaggtaaa
    23641 gaaataatcc aaaatggtat gaggcaagaa gttgatagat gtaaacaagc agtagaggaa
    23701 gcgaaaaaag gaaaaaaagt agcagttgta agcagtggtg atgctggaat atatgggatg
    23761 gcaggtctca ttttagaatt aatatctaaa gaagatgaag atataaaagt aaaggtaatt
    23821 ccaggtgtta ctgcaagtat tggtgcagca gctatacttg gagctcctat tatgcatgat
    23881 ttctgtcata taagtttgag tgatttaatg actccgtggg aagtaattga aaaaagattg
    23941 agattggctg ctgaagccga ttttgtaatt tgtttgtata atccaagaag taagggaaga
    24001 agtgaacatc taagcaaagc atttaaaata atgggtgagt ttaaaggtgg agatacacct
    24061 gttggagtgg taaaagatgt aggtagagaa aaagaagaaa agtttgtatg tacttttgaa
    24121 aatatggact ttgaaaaagt cgatatgact acaatggtta taataggaaa taagtctacc
    24181 tatattaatg gtgaattgat gataactcca agaggttata cagtatgatt ttaattttag
    24241 gaggaacttc agatagttta tctatatgtg ataagataaa tgaactgaaa aatcaaccat
    24301 atatcctttc agttactaca gattatggaa gggaacttgc acaacaacat gcagagaatg
    24361 taattttagg taagcttgga aaagaagata tgctaaaatt tataaaagat aataatatag
    24421 taaaaataat agatgccact catccttatg cagttgaagt atccaaaact gctattactt
    24481 gtgcaaaatt attgaatata gattatataa ggtttgagag aaaatcttta attgaagaaa
    24541 tagattatga taataaattt atagtagata caattgaaga agcctgtgaa atagcaaata
    24601 aaatagggac aaatatattc ataggaacag gaagtaaaaa tttgaacaga tttatagagg
    24661 aaataagtga taaaaattta gttgttagag ttcttccaac gagtgaagtc atattaagtt
    24721 gtgaaaaatt aggtttaagt gcagataata tagtagctat gaaaggtcca tttagccagt
    24781 atataaatga agaaacatat aaacattaca atatagattt agtcataaca aaagaaagtg
    24841 gaatagctgg tgggttctta gagaaggtaa atgcatgtaa aactttcaat ataccagtaa
    24901 taattataaa aagagaatcg attaactacg aaaatgtatt taatgacata aataaaatta
    24961 aagacatact tgtgtaattt ttaggaggag aatatatgaa gaaagcctta ttagtagtga
    25021 gttttggtac aagctatcat gaaacgagag aaaagacaat agatatatgt gaagataaaa
    25081 taaaaaattc gttaaaaagc catgattttt ttagagctta tacatcaaat atgataataa
    25141 ataaaataaa aaaaagggat ggaatagaaa tagataatcc aatacaggct ttagataaaa
    25201 tatatgaaca aggatatgat gaagtaataa tccaaacact tcatattata tgtggcgaag
    25261 aatttaacaa attaaaagaa caagtagatg gatattcttc taaattcaaa aagattgtat
    25321 taggaagacc tttgcttaca catattgaag attatcagga agctgtagag gcaattaagc
    25381 atcaaattcc tcatatggaa agtaatgaag cagtagtatt tatgggacat ggaacacttc
    25441 atgaatcaca ttcagcgtat ccagcattgg aatacatgct tagggataat ggaataaatg
    25501 cttatgttgg tacagttgaa gggtatccag aaatagaaca tgtaataaga agattaaaag
    25561 aaggaaatat aaaaactgta aatttgatgc catttatgtt agttgcagga gaccatgcta
    25621 taaatgatat ggcaggagat gaagaagatt cttggaaaac aatattagaa gaagatggat
    25681 ttgacgtaag agttcattta aaaggactag gagaaaattc ttatatacaa gataaatttg
    25741 ttcgtcatgc tgtaaaatgt gcagagaatg ctaagttcta tggtatagga gcagggccag
    25801 gagatggttc tttacttacg ataaaagcgg ttaatacttt aaagaaactg gatattctat
    25861 acactccaga atcaaaaaag ggtggagata gtctagcttt atcaatagtt agtgaatatt
    25921 tacctgaatc agtggaaata aagtctaggc atttcccaat gagttttgat gggaatgaaa
    25981 aaagtttagc ttgggacaat gtagcacaag aaattatatc agatgttaat gatggtaaaa
    26041 atgtaggatt tgttacatta ggagacccta tgatttatag tacatatgta tatataatga
    26101 aaagaatatt aggagaggta gaagtcgaaa ctataccagg aatttcatca ttttccaata
    26161 tagcatctaa tcagaatttt ccattagtca tggataaaga agctttagtg gtaattcctt
    26221 gtactatgga agatgataag atagagtatg ctttgcaaaa ttataattca atagtattga
    26281 tgaaggtata taaaaatttt aaagaaataa taaaaaaatt agaaaagtat gatttaatag
    26341 aacatgctat tttagtaagt aattcttccc aagaaagcga aaatgtgttt acagatttaa
    26401 aagaagcgca ccttgaagat aagatatctt atttttctac tatactagta aataaagata
    26461 ataaaataaa ataggtgaaa gcaatattta aaggagactt aatatgaata tagtggttgg
    26521 aactaggggt agtactctag cgcttataca aacagagtgg gtaataaatg aattaaaaaa
    26581 gaagtatcca gaaattagtt ttgagattaa aatcataaag acaaaaggag atttaattca
    26641 aaatgtttca ctggataaaa taggtgataa aggattgttt gtcaaagaaa tagagcaaca
    26701 attattagat ggaaaaatag acatagcagt acatagtatg aaagatatgc cttcttattt
    26761 agcagatgga ctaaaatttg ctcatacgcc taagagagaa gacccaagag atgttctaat
    26821 acttagagaa ggatataaaa atttagatga tttgcctcat ggagcagtaa taggaacagg
    26881 aagtaaaaga aggaagtttc aattattgaa acaaagacct gatttaaata tagtacaagt
    26941 tagaggtaat gtggaaacta ggatacgaaa aataaaagat gaaaacatgc atggtattgt
    27001 gttggcagca tcaggaataa ttagagctaa tttgcaagat aaaataagtt cttatttacc
    27061 tgttgatgtt gttattccag cacctgccca aggagcttta gctatagaaa ttagaagcaa
    27121 tgatagtacg atagagggaa tagtaaattc actgaaggat gagaatacag aaatccaaat
    27181 attggctgaa agaggttttt tagatggtgt aaatggaagt tgtcatattc caatggcagc
    27241 ttattgtgaa ataatccaag ataaaataca tcttactggc ttatatggag attcagaagg
    27301 aaaaaaagta gtaatcaagt ctatagatgg agatatttct tctcctagag agttaggttt
    27361 aaagttagct aaacttgtat tgaaggagta tgagaattat gaagggtaag gtttacttag
    27421 taggggcagg accaggtgat tataaactca tgactttaaa aggtttagag tgtataagaa
    27481 aatcggatgt aatagtatat gatagattag caaatagtag ttatttaaga gaagctaagc
    27541 ctgattgtga acttatatat gtaggcaagg catcaagtaa tcatattttg ccacaagaag
    27601 atataaatag aattatagca gaaaaagcta aagaaggaaa aattgtaact cgtcttaaag
    27661 gaggagaccc ttatgtattt ggcagagggg gagaagaagc agagacttta gtagatgaag
    27721 atatagaatt tgaagtagtt ccaggtataa cttctgctat aggaggttta tgttatgcgg
    27781 gtattcctat aacacataga gaccatgctt cgtcatttca tgtaataaca ggtcatttga
    27841 agggtgatga tagtggagaa ttaaactgga atgctcttgc aaataacaaa ggtactcttg
    27901 tgtttttgat gggaatttca aacctagaga aaataagtga aaacttaatg aaagaaggaa
    27961 aagataagga tacacctgtt gctcttataa gttgggcaac tagatataat caaagagttg
    28021 ttacatctac acttgagaat gtttatgaaa ctgctattaa agaagaagta aaaccgccaa
    28081 ctcttatagt agttgggagt gttgtaggac ttagagaaaa gttgaacttc tttgaaagta
    28141 agcctttatt tggaaaaagt attgctgtaa ctaggtctag aaatcaaaat agtgtattgg
    28201 tagaaaaaat aatggattta ggtggaaatc ctatagaaat acctactatt aaggtagaaa
    28261 aaatacaaaa taatattaac ttagaaaatg aaataaaaaa tataaataag tataattatt
    28321 taatactaac tagtaaaaat gccgtagaaa tcttctttga aaagatatat gaaatgaatt
    28381 ttgatttaag gatattatct aatttaaagg tatgtgctat aggttctgct acatcaaatg
    28441 agttgaaaaa aagaggaatt atagcggata tagtaccaaa aaaatttgta gcagagtcac
    28501 tgtatgaaga gttagctcct attttaaatg ataacgataa tatacttata cctagagcag
    28561 aaaatgctag agattatctg gttgacaaac ttaaggctat ttgtgaagta acagaagtac
    28621 atatatatag aacagtaata gatgaggata aaaaaggtga gatattagat attttaaatt
    28681 ctaatgatat agattatata acatttacta gttcctcaac agtaaataat cttgttgaaa
    28741 ttataggaaa agaaaatatt aaaatattag aaaaaataaa aacactatct ataggtccta
    28801 taactacaga gacaataaat agtttgggaa taaaattgta caaagaatct gctacatcta
    28861 ctatagattc gttagtagat ttaattataa aaaattaatt ttggaggctt gaatatgtta
    28921 agaagaccaa gaagactaag agcaagtaaa gcaatcagaa atttggtaag agaaactaag
    28981 gtgaatgtag aagatttaat atatccactg tttgtagtag agggggaaaa tataaaagaa
    29041 gaaatcaatt ctttaccaga tgtatatcat ttatcaatag acatgctaga agacgaaata
    29101 aaagaaataa gtgagttggg aattgaacat ataatgttat ttggtgttcc agatgaaaaa
    29161 gatgagtgtg cttcacaagc ttttaatgat aatggaatag ttcaaaaggc aattagaaag
    29221 gtaaaagaaa taaattcaga tataaatgta ataacagatg tctgtatgtg ccaatataca
    29281 agtcatggac attgtgggat tttaactgat actggttatg ttgataatga taaatcattg
    29341 gagtatttgg ctaaaatatc tgtaagccat gctaaagcag gagctgatat ggttgctcca
    29401 tctgatatga tggatggaag aatacaagca ataagagaag ctttagatga aaatggatta
    29461 gaaaatgttg gaatcatggc ttatagtgtt aagtatgctt caaattatta tggacctttt
    29521 agagaggcag ctcattcagc accatctttt ggagatagaa aaacatatca aatggaccca
    29581 gcaaatagca atgaagctat aatagagact gaacttgata tattggaagg tgcagatata
    29641 cttatggtta aacctgcatt atcatattta gatattataa gaagagttaa agataatttt
    29701 gacttacctc tagcagctta taatgtaagt ggagaatact ctatgcttaa gtttgctgtg
    29761 aaggaaggtt tattaaatga agatgtaatt cttgaatcaa ttacatctat aaaaagagca
    29821 ggagcagata taataataac ttattttgct aaagacattg ctaaactgtt gaaaaaataa
    29881 attctagtag tataaagaat atcaaaaagt ataggaattg taattaacta ttatacagta
    29941 gttttgttta gacatttatt gaagaataat agaaactaat ttacaatttc tatactttat
    30001 ttatgtaact aaataaaatt tggaggtaga agatgctata tcctattaat ttaaaattag
    30061 atgagttaga tgtagttata attggtggtg gagaagttgc atataggaaa tgtaaaaatt
    30121 ttttagagtt taacaaaaat gttactatag tttctaaaca aattttaaat aaattttatg
    30181 atttaaaagg aaatataaaa ataataaaag atgattataa agaaatatat ataaaagatg
    30241 cttctgtaat aattgctgcc acaaataata gagagttaaa tatggaaata ggattatatt
    30301 gtagagcaaa aaataagcta gtgaatgtag ttgataatat agaaatatcg aactttactg
    30361 ttccatcata tataaaaaga ggagatttac ttataagtgt gtctactggg ggcaagagtc
    30421 cttcattatc atcaaagata aaaaaagaaa ttgaagagag atatactgaa gattatgagg
    30481 agtatttaaa tgtattagga gatataagaa aaaaggttat aaaaaaatac gaagataaaa
    30541 gtaaaagaaa agatgtgctt aatatgttgg taacacttga cctagaagaa cttaagaagt
    30601 ttgatatata aaaaataaaa taaaaaatgt taaaaaggtg ttgacggatt atttttaaga
    30661 tgatatagt
//
LOCUS       DAMJVX010000026        30626 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMJVX010000026 DAMJVX010000000
VERSION     DAMJVX010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30626
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.028"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(55..216)
                     /locus_tag="PC407_001160"
     CDS             complement(55..216)
                     /locus_tag="PC407_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496618.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     gene            complement(557..997)
                     /locus_tag="PC407_001161"
     CDS             complement(557..997)
                     /locus_tag="PC407_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2496619.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            complement(1069..1896)
                     /locus_tag="PC407_001162"
     CDS             complement(1069..1896)
                     /locus_tag="PC407_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496620.1"
                     /translation="MEFESGIKLGKLTLIKYLGRNKHSKKVWLCQCECGNKVERVENN
                     LKQSIKNEKPPHCGCSPNWKGQNKRFKDITGKTFGKLTVLKMSGKDKYSHNLWLCQCE
                     CGNRTITSTSALEQGKAQSCGCIRKEILLERSTTHNKSNDSLYRVYHRMIKRCTNKSN
                     KDYNYYGGRGIEVCNEWLEDFINFYNWSIENGYRKGLTIDRINVNGNYEPSNCRWVTW
                     EVQRNNKRNSIRVNYKGEMITLKQCAENLNVKYLFLYNQLVTKKIPLEEVIKNIGME"
     gene            complement(1955..2425)
                     /locus_tag="PC407_001163"
     CDS             complement(1955..2425)
                     /locus_tag="PC407_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2496621.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVESFALLNGML"
     gene            complement(2442..3752)
                     /locus_tag="PC407_001164"
     CDS             complement(2442..3752)
                     /locus_tag="PC407_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HDF2496622.1"
                     /translation="MALGGGTFVTQNKILPGAYINFISAKRATSSLSDRGIVAMPLEL
                     DWGIDEEAFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVILLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSIENKIKDKDLVESSLIYWATGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAIKVINAMSKLYMTVSVN"
     gene            complement(3753..3929)
                     /locus_tag="PC407_001165"
     CDS             complement(3753..3929)
                     /locus_tag="PC407_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496623.1"
                     /translation="MSKALSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEDRINKFMKGVS"
     gene            complement(3922..4359)
                     /locus_tag="PC407_001166"
     CDS             complement(3922..4359)
                     /locus_tag="PC407_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496624.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPRSEDKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKNNDTNKMDTVELFGGLKRGDNFE"
     gene            complement(4708..5514)
                     /locus_tag="PC407_001167"
     CDS             complement(4708..5514)
                     /locus_tag="PC407_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496625.1"
                     /translation="MAKFLDITGKKFGRLRVIKFSKEIKSGKRNRKYWLCKCDCGNFK
                     EIRTDSLTSGLVQSCGCLKKEQDKLNLTDKYQFKKKYKVQNKRLYSIWKGIISRCTDK
                     NNKRYNRYGERNIIVCDEWFCYDNFANWALSNGYSEKLTIDRINNEGNYESSNCRWVD
                     IKTQCRNRSTNILVKHEEKEITLIELSEKTGISYSCLRSRYSKGLVGNKLIEKVKIIE
                     ESRAKLSIEDVKEIRKKYSDGYTIKQLSEIYPVTYSSISNIVHRRTWKNI"
     gene            complement(5624..5914)
                     /locus_tag="PC407_001168"
     CDS             complement(5624..5914)
                     /locus_tag="PC407_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF2496626.1"
                     /translation="MARWGSVDFREFKRVCKKMEKFTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLREGWSGVAYARSLPVYKQGNNYIIEVVNPTEYALTQWGI"
     gene            complement(5914..6261)
                     /locus_tag="PC407_001169"
     CDS             complement(5914..6261)
                     /locus_tag="PC407_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496627.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKLAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(6255..6656)
                     /locus_tag="PC407_001170"
     CDS             complement(6255..6656)
                     /locus_tag="PC407_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496628.1"
                     /translation="MENNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNKILTFRCLRW"
     gene            complement(6666..6899)
                     /locus_tag="PC407_001171"
     CDS             complement(6666..6899)
                     /locus_tag="PC407_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496629.1"
                     /translation="MPQVKKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLSV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(6918..7808)
                     /locus_tag="PC407_001172"
     CDS             complement(6918..7808)
                     /locus_tag="PC407_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDF2496630.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATENNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTANVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDGGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEA"
     gene            complement(7820..8383)
                     /locus_tag="PC407_001173"
     CDS             complement(7820..8383)
                     /locus_tag="PC407_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HDF2496631.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTVTQEDYVNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDMEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            complement(8451..8699)
                     /locus_tag="PC407_001174"
     CDS             complement(8451..8699)
                     /locus_tag="PC407_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496632.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            complement(8701..10236)
                     /locus_tag="PC407_001175"
     CDS             complement(8701..10236)
                     /locus_tag="PC407_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDF2496633.1"
                     /translation="MKHKDYWRKRFEQLEEVQNNKSVKYYLELEKKYKLAMNSIEKDI
                     LSWYNRFAENEGISLLEAKKLLNTRELEEFKWNVEEYIKYGKENAINQKWMKELENAS
                     ARLHITRLEALKLQIQQHVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWATDGLNFSERIWGKHRPTLINELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFSDLDVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKHIEGRLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYND
                     TIRYEEAKKLYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGN
                     TGKPRLDLDLTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDIL
                     NGE"
     gene            complement(10226..11668)
                     /locus_tag="PC407_001176"
     CDS             complement(10226..11668)
                     /locus_tag="PC407_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2496634.1"
                     /translation="MLNIYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEMQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKSVQITFNHSMIINESEKIDMASKSIGIISDETIVSNHPWVEDVNDE
                     LERLKKQEETQKEYDDLIPTNNQEGVIDET"
     gene            complement(11674..12927)
                     /locus_tag="PC407_001177"
     CDS             complement(11674..12927)
                     /locus_tag="PC407_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDF2496635.1"
                     /translation="MVIEMIVRINFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANAIL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSRGLEKTLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            complement(12905..13597)
                     /locus_tag="PC407_001178"
     CDS             complement(12905..13597)
                     /locus_tag="PC407_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2496636.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWSSE
                     KKKGAPINKRGAPFSNKNSVGYGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHEITENSEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVANLNKDDIGDDEL
                     KISVDYGDRNDS"
     gene            complement(13667..14176)
                     /locus_tag="PC407_001179"
     CDS             complement(13667..14176)
                     /locus_tag="PC407_001179"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496637.1"
                     /translation="MYKKVLNILLNYYKAINSKEVVELVLTPVLICIISYMFFNSSIN
                     KEVVISLNKDVLTLSGLLVAFGVCIITLLFTTYNKSISEAKDIKTERKVNGFNISYFQ
                     FIQLKAYYTVIMEILVVVICFINTIMLTRYYSNIIFYVLIFFTVHIILSLTTLIISMF
                     HLSWKDRGD"
     gene            complement(14197..15171)
                     /locus_tag="PC407_001180"
     CDS             complement(14197..15171)
                     /locus_tag="PC407_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496638.1"
                     /translation="MSNFKKRYIDVYRIDIINKINGSVYSDEEILKEVIKTFFKDKEY
                     CKENKNGINITKDKKFWITEVKCSEDDLIIKVKLEYTKYNQNTNIINAHTKEVEGNKK
                     MDQGDSNKQHLFIKFMKENNIAVVLFERVFVGVPMIELSKNLHLYYEDMIENNKFDNN
                     MSKTSINITQIASKDFIEQILDLDGVSKITLNVDREKFGTDEDNLFSNLNNSRRHNDL
                     IYKPTFRTRYLPSEVKRCCEKYIKGEEFRNKKINRIIIEGEKNKRKLKLDTEGIKLCR
                     EIEVSLDIDNHIDSEDIFEKMNNLIDDLIENNSVFFNLMYQEVAMSEA"
     gene            complement(15366..15915)
                     /locus_tag="PC407_001181"
                     /pseudo
     CDS             complement(15366..15915)
                     /locus_tag="PC407_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
     gene            complement(16198..16677)
                     /locus_tag="PC407_001182"
     CDS             complement(16198..16677)
                     /locus_tag="PC407_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HDF2496639.1"
                     /translation="MDKESIKEIVRELRKEEREDRKKGVLYNTRLLMKHYNDFKRHID
                     SAVSEAKDVDYLEDDLGKLDNEELYILSIKKSKARTIIMIAHIDSAMKTLKLRQERLH
                     SYEKYRALELYYCDEKTYEEIAEILNCGVVTSRRWINEMVKELGVYLFGIDGLKLTV"
     gene            complement(16670..16807)
                     /locus_tag="PC407_001183"
     CDS             complement(16670..16807)
                     /locus_tag="PC407_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496640.1"
                     /translation="MELISPGKIVRCAWCGRRFYKLDKSKVEYCSRSCSKRARRSDLS
                     G"
     gene            complement(16831..18201)
                     /locus_tag="PC407_001184"
     CDS             complement(16831..18201)
                     /locus_tag="PC407_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2496641.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCTLTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKNGAENA
                     IHNKIKDICISLKAEDYLNMPRKIDNKIEIHLDSKILKYYKELEKEKILELDRDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEAIENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            complement(18150..18476)
                     /locus_tag="PC407_001185"
     CDS             complement(18150..18476)
                     /locus_tag="PC407_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDF2496642.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKLRKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            complement(18758..21175)
                     /locus_tag="PC407_001186"
     CDS             complement(18758..21175)
                     /locus_tag="PC407_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496643.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIENFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYTRTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDTGVKKVNKSIFNGQLDNERNQIWAEAVELCKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITENWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            complement(21187..23145)
                     /locus_tag="PC407_001187"
     CDS             complement(21187..23145)
                     /locus_tag="PC407_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HDF2496644.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTAISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWFAGEKWRLDVFNSHGKIYEASASHMFKVPIESIKKGDPLRQKGKISELAL
                     GYGGSIGALTSMGAIKMGLDEEELQPLVTTWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VKLQHGIKFIYNPGVLFIELPSGRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            complement(23145..23717)
                     /locus_tag="PC407_001188"
     CDS             complement(23145..23717)
                     /locus_tag="PC407_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HDF2496645.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            complement(23740..24312)
                     /locus_tag="PC407_001189"
     CDS             complement(23740..24312)
                     /locus_tag="PC407_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HDF2496646.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            complement(24365..24700)
                     /locus_tag="PC407_001190"
     CDS             complement(24365..24700)
                     /locus_tag="PC407_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496647.1"
                     /translation="MPLDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     RLNITEYNSTEENAKVIVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            complement(24715..25881)
                     /locus_tag="PC407_001191"
     CDS             complement(24715..25881)
                     /locus_tag="PC407_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HDF2496648.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPLTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            complement(25893..26330)
                     /locus_tag="PC407_001192"
     CDS             complement(25893..26330)
                     /locus_tag="PC407_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496649.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            complement(26401..26550)
                     /locus_tag="PC407_001193"
     CDS             complement(26401..26550)
                     /locus_tag="PC407_001193"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496650.1"
                     /translation="MKLLSLDTNNKNVVTLKKDGKVIADIVFKDIKTGKKISVGILNK
                     KVLVK"
     gene            complement(26531..26923)
                     /locus_tag="PC407_001194"
     CDS             complement(26531..26923)
                     /locus_tag="PC407_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2496651.1"
                     /translation="MDISESIAKQFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVEWGDEIAES"
     gene            complement(26954..27151)
                     /locus_tag="PC407_001195"
     CDS             complement(26954..27151)
                     /locus_tag="PC407_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2496652.1"
                     /translation="MLIGDNIKQMLRKRDIKPYKLAKELNIDVSGLYKLLRNKSSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            27227..27601
                     /locus_tag="PC407_001196"
     CDS             27227..27601
                     /locus_tag="PC407_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496653.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            complement(27611..27820)
                     /locus_tag="PC407_001197"
     CDS             complement(27611..27820)
                     /locus_tag="PC407_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496654.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMNKSEKRKISEITKERIRK"
     gene            complement(27867..28676)
                     /locus_tag="PC407_001198"
     CDS             complement(27867..28676)
                     /locus_tag="PC407_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDF2496655.1"
                     /translation="MNNLQIFKNKTFGEIRVIELNGEFWFVGKDIAEQLGYKDTSDAL
                     KRHVDDEDKGVGEIPTPGGNQNMKVINESGLYSLILSSKLPSAKLFKRWVTNEILPSI
                     RSTGTYNMIDLQTKLPTTYKEALQHLIEQVEVNEQLQLESKMKDQVIKELKPKADYTD
                     MILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHSET
                     IDITRSNGMPDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKGYSYQTSILG"
     gene            complement(28724..28927)
                     /locus_tag="PC407_001199"
     CDS             complement(28724..28927)
                     /locus_tag="PC407_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2496656.1"
                     /translation="MKKRSLKAGRIEAGYTQEELAYKIGIAKSTYNLKENGKRNFTEK
                     EMIMISYILNKTMDELFLEKRLT"
     gene            29104..29445
                     /locus_tag="PC407_001200"
     CDS             29104..29445
                     /locus_tag="PC407_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2496657.1"
                     /translation="MATFGERFKQLREEKKLTQDELVSKFNKVYFTSFNKSTISQYEN
                     NKRKPEINILENWADFFDVSIDYLLGRTLVRNHIDTVAAHRVNPHKDLPEEAQEQLND
                     YIEFLMNKYKK"
     gene            29457..30005
                     /locus_tag="PC407_001201"
     CDS             29457..30005
                     /locus_tag="PC407_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2496658.1"
                     /translation="MLILKKLRTEKGISLDKLSTDLNINKSTLSRIENGLREPKESFI
                     KDCSDYFGVSTDYLIGKINIDDSNKLTKCLNSTFPIRLKELRKKKELTQAELSKLLNC
                     SLSKIAMLETSKREPVKEDLLRISEFFNVSVDYLLGKTSIENYITTDEISKIIKSYES
                     LPKEAQEHINSYIEFLVDRYKK"
     gene            30081..30587
                     /locus_tag="PC407_001202"
     CDS             30081..30587
                     /locus_tag="PC407_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDF2496659.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
BASE COUNT         9153 a         5789 c         3091 g        12593 t
ORIGIN      
        1 gaactacaat ctatttgaca gtagagtgaa gttcataatt taaacaatta gtaattattt
       61 aaatttgtgg aacttgattt taaaatcaaa ttcccagcca cttttactct tgccacgagt
      121 tgagtggctt tttactttta gaaatacttt acaaattaag caaaatatta aactagcaat
      181 aacgccagct attacattaa gtaaaaagtt attcatactt cccacctcct ttcattagga
      241 agtaggtttt atcccagtat gaactccact ctataaattg tagattacat cttcttgcta
      301 aaaatattat aacatataat tattacatat tttacctatt ctatatttat ttttttattt
      361 tgctatcttc ttcgtcccct ctttctctct ctttcagctt ctttcattgc ttcctcttca
      421 tcttctatct ttataagtat tgaggcggct gctaacgctc tctcattaac ttctaaattc
      481 atatattcac ttggcttcca ctttaatttt tgaatacaat aatgagtgat gctagcatca
      541 aaatcgccac ctctgattag ttttttgctt cttctacttt atcctcaaaa gatgtatcaa
      601 atccattgac ttcattcact tttactgtat aattgacata ctcacctgct gtaagcattg
      661 tctttaataa ctgagcttct cccattactc cataactatt ttggagttcg gcatccttta
      721 aatctggaaa tactgtagat gctacacata attcagctac ataactattg tagtcaattt
      781 cacttgtata ttgtccagta tgcttaccat tgttaccaat cacttttact cttttagtac
      841 actttcttct tagtgcttcg tcttcttcag atgataaaac ttttaattcc cattcaactg
      901 gtttcccttc tttatctaaa aatctgttac tcgctacata ttttacatta tcaaccttta
      961 ttgcattttg acttaaaaaa gcacttaaat tactcatatt attctaatct ccttttattt
     1021 taatttttca tataaaaaat acacatttat aacttataaa tgtgtatttt actccatacc
     1081 tatatttttt attacttctt ccaatggaat ttttttagta actaattgat tataaagaaa
     1141 tagatactta acattcaaat tttctgcaca ttgttttaaa gtaatcattt cacctttata
     1201 attaactcta atactatttc ttttattatt tcgttgaact tcccaagtaa cccatctaca
     1261 atttgatggt tcgtaatttc cattcacatt aattctatct attgttaaac cttttctata
     1321 tccattttct atagaccagt tataaaaatt tataaaatcc tctagccatt cattgcatac
     1381 ttctatgcct ctacctccat aataattata atctttatta ctcttatttg tgcacctttt
     1441 aatcatacgg tgataaactc tatataagct atcattagat ttattatgcg ttgtagagcg
     1501 ctctagtaat atttcctttc ttatacagcc acaactttgt gcttttcctt gttctaatgc
     1561 acttgtacta gttattgttc tatttccaca ttcacattga cataaccata aattgtgact
     1621 atatttatct ttcccagaca ttttcaatac tgtaagcttc ccaaaagttt tcccagttat
     1681 atctttaaat ctcttatttt gtcctttcca gtttggagaa caaccacaat gtggaggctt
     1741 ctcattttta attgattgct ttaaattatt ttctactctc tcaactttat tcccacactc
     1801 acattgacat agccagactt ttttactatg tttatttctc cctaaatatt taattaaagt
     1861 aagtttgcct aatttaattc cactttcaaa ttccatgaaa tacctccaaa taatttatac
     1921 ttacatcata atatttttca agtataattc aattttataa cattccatta agtaatgcaa
     1981 aggattcaac taattcccaa tcctcaaaag tgaaatccat atcttcatct aaatactcac
     2041 catcagcatc aaatttagta attattccac tgtccatatt acaatcttta agtactactg
     2101 tctgtcttcc tacagcagat gtagggtctt catttgtaac ttgtatgtca aaataaatat
     2161 cctcaccagt ttctttatat ctgtaaagta attctctaaa aatagaagta ttataatgaa
     2221 atgttgcact tccagtattt gtactcccag ttgttttatt tccctttgtt gttcttccta
     2281 gaattggaac ttcactttta tttttttcca ttttagcctc taaatctata gcttgcataa
     2341 aattatatct tttaccttct atagttataa aacattcagc tttctttgca ctaactgtat
     2401 ctttagcatt tattgtttga gccatattat cactctcctc tctaattaac tgaaacagtc
     2461 atataaagct tactcatagc atttattacc ttaatagcat cagatactat gacagttttc
     2521 ttatcatttc caagctctac actaacatca tcagttttaa aatcttctat tgcccttata
     2581 ttctctaatt ctttatggtg tttaacaaca tcattccaga aacttattct tcctgcctta
     2641 tcattcggaa ctttacctaa atacttttca ttaaataaag ttgcaatatc attagcaatt
     2701 tggtcaagta ctctaacact ttggttactt gaaaaatcgt catttttatc atctgtaaat
     2761 gatacaaaag tatttatatc ctctaacaca tgaacttcat ctcccacctt gtggaatata
     2821 aatttaccag tttttaaagc ttcttcaagt tgtatttgtg tataattaac atcaacatca
     2881 aactcaccat catacttttt attagtatta gatttattta tatcgcatcc tgctatagct
     2941 ccagtagccc aataaatcaa gctagattca actaaatctt tatctttaat cttattttct
     3001 atagacacta caccttcata atctgcatca cttttcttat atagtactgt ttgaaactta
     3061 gctcctacct tatctctcat tctctttgta aattctacaa ataaactttt aatttctgtt
     3121 gttgtagcca aacaccctag tgcattaaat gaataacttt ctattttatc caagaaagct
     3181 tggtactctg ctcctgtcac agcttcgcca ttggttccac cagtaaatac aagtcctgca
     3241 cttgcttcta gtgttgcatc cttcttccaa gtgatatagt cattgtcttg taagtctgta
     3301 ataacctttg ctatttgagt atctaccttc ttattatcta aaagtattac aacatcaaac
     3361 ttagcattat catctatatt tgttgtaact gttactttta agtcatttcc tcttatacca
     3421 ctatattttg ctgtggctat agtacaactg gctttaacgc ctttatttaa tttataaaaa
     3481 tatcccaacc ttatattttt gaataaatct ctcaaacctt tcagcttctc atgagtataa
     3541 tcatatccaa aatacttcac tgaatacttc tcaaaatcat cactggttac ttggaatgct
     3601 tcttcatcta tgccccaatc taactctaaa ggcattgcaa caatacctct atctgataat
     3661 gaactggttg ccctcttagc tgagataaaa tttatatatg caccaggaag tattttattt
     3721 tgcgttacga atgttcctcc acctaaagcc atctaactca ctcctttcat aaatttattt
     3781 attctatcct ctacctctga gaaggaatat aactcatttt cttttaaaat tgcatttaat
     3841 aaatcttttc tatttacata cttcttagag ttaactatct gctccttagt aaacttgtag
     3901 ttatcttctt tgcttaatgc tttactcaaa attatcacct ctcttcaaac caccgaataa
     3961 ctctactgta tccatcttat tggtatcatt attttttata gtaaaatagt tataatcaac
     4021 aaagaagtga agaacattgt ctataatttc aaaattcata tttgtacctc ttactaaatc
     4081 tccatcaatt tctatatact ctaattcctc cagtagcatc tcagctatct catttatttc
     4141 aaaattctta tcttctgaac gagggaaata atgtacatca aaagaatttt tctttaattc
     4201 tctcccgctt ggatatggtg tcttgcttgg atttaaagga acaataaaaa aacaaggttc
     4261 attaatacct tgctccacat cttcgctata aattgtatat ttttctccaa acgatttatc
     4321 taactttact gatattccat ctataatatt attaagcatc aaacactcct ttaagcaata
     4381 ttaatagttt tttctctatt atcttatcaa cttgactttg tagttccatt tctgaaatag
     4441 ttaagaaatg ttgtccttta acccaaccct tgccattttt agttctatga ccaaattcaa
     4501 catatgcatt ttgttatcct aaaggctttt tatcctctag ctcttatagt ttcctataag
     4561 ttcggcgtac atcatcaaca aaataaactt tatttagttg cccagcactc ttggagagat
     4621 tatatttatt cactctctac gctctacgga aacctatagc ctatccgcaa tctataggtt
     4681 ttcctcggta ttggcatata taattaatta aatatttttc catgttcttc tatggactat
     4741 atttgatata gatgaatatg ttacaggata tatttcactt agttgtttta ttgtatatcc
     4801 atcagaatac ttttttctaa tttcttttac atcctcaata gataacttag ctctgctttc
     4861 ttcaataatc ttaacttttt ctataagctt atttcctact aatcccttag aatatctact
     4921 tcttaagcaa gagtatgaaa tacctgtttt ttcagaaagc tctatcaatg taatttcttt
     4981 ttcttcatgt tttactaata tatttgttga cctatttcta cattgtgtct ttatatccac
     5041 ccatctacaa ttgcttgatt cataatttcc ttcattattt attctatcaa ttgtaagttt
     5101 ctctgaatat ccattactca atgcccaatt tgcaaaatta tcatagcaaa accattcatc
     5161 acacacaatt atattccttt caccatatct attgtatctt ttattatttt tatctgtaca
     5221 tctagatatt atacccttcc atatactgta aagtctttta ttttgaacct tatatttctt
     5281 tttaaattgg tatttgtctg ttaaatttaa tttatcttgt tcttttttta aacatccaca
     5341 agattgcact aagccactag ttaaagagtc agttcttatt tctttgaaat ttccacaatc
     5401 acacttacat aaccaatatt tcctatttct tttaccgctt tttatctctt tagaaaattt
     5461 aatgactctt agccttccaa atttttttcc tgtaatatct aaaaattttg ccataaaatc
     5521 acctctttta tacattataa atcaagagtt attctatgtc catattctac ctactttata
     5581 atgtaacact attacactta gcgtttaccg attttgctgg gttttatatg ccccattgtg
     5641 ttaaggcata ttcagttgga ttaacaactt ctataatata attattacct tgtttgtaaa
     5701 caggaagcga cctagcataa gctactccac tccaaccctc tctcaaaaat cctgtatcaa
     5761 ctggtgtcct tctaattact tttcctaaga gtcttgctgc taattctctt gctgcatcct
     5821 tgcaaaactt atctaaatca atctttgtaa atttctccat ctttttacaa actcttttaa
     5881 actctctaaa atcaacactg ccccatctag ccattatgct ttatccttaa ataactcaag
     5941 tattatttct tgatgatttg gatatatagc tgattctcca cttcttacat actctttatc
     6001 atttataata agttttgaac ctgctttaat ttctatatct ggagatataa agagtttaat
     6061 agtttgctct agcttagcta atttcccttc tgtagcagaa actatatttt tatatgaaag
     6121 tttacatggt tgattttcta atacaatcac ttctttattg ttagttcgtt ttgttacagg
     6181 gtctttaatt ggctgatact caactatagt acatttatct ctatataaca tttctattgc
     6241 ttttctagtt ttacttacca tcttaagcac ctaaaggtta atatcttatt cttaccataa
     6301 gtagtaagat aagctattaa gctatcaaag cgttgttctg gtgtttgaga accacttcct
     6361 atagcaaaat ctacctttgt atcaccttct gatatagact tttctacagc ttcaaagtta
     6421 atgctttcta tatctaattg acccatattt ttcttggtaa ataagaactc tccaactatc
     6481 atatcaactt caatttcttt caattcaatt ggcatagttt ttatattaca atctagttta
     6541 ataatatttt ctattttttc tcttacaaaa cctattaacc acttatctcc atcttttaat
     6601 atatatccaa aactttcaag tcttttttct atttcatcaa ttatattatt ttccataatt
     6661 ttcacctact ttttagtaag tttatttttc tctttaagct gcttattttc ttcttctaaa
     6721 gactcaactt ttgaccttaa aatattattt tcagctatta aatcttttac acttaatgac
     6781 ttgccatact ttactgcctt accagtttca tctatcaaat catatcccat ctctaagaaa
     6841 tcatctattt tacattcttc tatggttagt attctattta gtttttttac ttgtggcact
     6901 atatatcact cctttttcta tgcttcaaca acaaattgta ttgcatcagc ttttttattt
     6961 aatataaata catcctcaaa actttcttca aagtagaagt attttccctc tgtaactgct
     7021 gttggttcgt ctaacttaga gaactgataa gaaacaggtg taattattgc acttgggtga
     7081 actaaggaca taaagatttg tttagctcct gctcctactt tccatccagt tgtaaaatca
     7141 tatgcagttt tcattagatt agatggtact ttaattattt taactgtgtc aatatctgtg
     7201 gtttgtctat ttaaagaagt tccaccatct tttatgttta ctgttctttg tatctctttt
     7261 gcatttttga taagtgtatt tactactgga gtaacataca atattcttcc attttcaggt
     7321 actctagctt ctgtcatttt ttccattaac ttatcaaata cttctaatac gtttgctgtt
     7381 gtaagaacag ttgtatctgc tgtgttacct aatgcggtcc aatcagcata tattttagat
     7441 atacagtaag catccatctc tggaaacttt tgttcctcat tatatacttt tgttatatta
     7501 cctattgaag ccacataatt agtttggttt atatctgctg gatgaaccaa tgttgaccat
     7561 ttcctttgat tagttaatac cttaggttcc caagcattat catagtttct ttgagctact
     7621 gctattgtat ctctgtttga atctaccctt ccagttgtag atatagttgg tatttctatt
     7681 gttttagaac ccgtccatct atatcttcca ttattttctg ttgcatacaa atccccgaag
     7741 tttaaagtat aaggatatgc ttgtgctaaa acatttgaat attcttttgc ataatttagt
     7801 gctgccattt tatttcctcc tatttattat tattttcatg aggtcttacc ccagtaaaat
     7861 taaaaccaaa atcatttatc ttaggctctt gccctggtgt tatagtatct attttaggtt
     7921 cttcaccttc tagtgttgca ttaaacaaat aatctttatc ctgtttcaaa gggtttattt
     7981 gctcttcaaa agctttttgt ctatctttac tatttcttag ggcttccatg tctaaatgag
     8041 cttttaacgc tatttcatct ctacatttaa cagatttaaa agcatcacct aaccagtaat
     8101 taaaatcttt ttcttcaatt tcttttttgt aggtttcttc caaagttttc ttatcagttt
     8161 cataagttgt ttttagattc tctacatctt cttttgtcat acctccttca aactttttaa
     8221 tagtttcatt agctgtatta agttgtgttt caagatttac ataatcttct tgagtaactg
     8281 tagtctcctt tattttcttt tctatagatt tttgaagaga agctacatca attttgttat
     8341 cctctacttt tattccttct agcaattctt ttaaccaatc cattttaaat ttctcctttc
     8401 attttttaca aaataaaagc acctactaat ttattattta gcaagtgctt ttacatattt
     8461 actatttgta tatctttcca taaatccttt agtaatttac catcaatgtt gtaattatca
     8521 accatatcct taccattttt ataatacttt gtatctccat tagggcaaag agtaataaac
     8581 cttgtatcat catctccaat agatatatta tatggtttat tatataagtc aaattctata
     8641 tctaatccta aatcaataga atcaattaaa tgttgtaaat tcttaaattt attatccatt
     8701 ttattctcca ttcaaaatat ctttgttagc tattttatga gcttttgtaa gctccatatc
     8761 cttctctctt tttaccttat catggttatt ttcatcagct aaccaatcat gtttatgagg
     8821 tacaatttta tgttgctttg ggtttctatg gtcagttaag tctaaatcta atctaggttt
     8881 tcctgtatta ccatagtatc ttctttgaat taatttacca tctttgtaat tatcaaatac
     8941 actatttggt tttgattcaa atggcactga atgaacactt ccactagtta aatttctctg
     9001 attcttaact tgccaattta catccttata aagcttttta gcttcctcat accttatagt
     9061 atcattatac ttcatatgtt gatattcatc aaatttagaa ggtatttcat ttcctaatac
     9121 ctttttatat tcttcaaatt gttttctatc tttactctca tttagttgca tttttctaag
     9181 ggtgtcagct ttttcttttc caagtctacc ctctatatgt ttctcatacc actcattata
     9241 cttcatacta gatggtacat aatatgtttt tccatcttct cctcttgctg ctctatagcc
     9301 ttcttcatcc tcgaaccaag gagctgttgt tgtcctacaa cgacaatgaa atggtggagc
     9361 tgttattcca acttgataat ctttcatatc aaatattttt ccatctaact ctctgcatat
     9421 atttgaagtt cttaaatcta atgtagcaat aatctcatat ttctctacat ctaaatcact
     9481 aaaacaatct tttctacttg ctgatgcaaa gaaagctgat tcagtcatta tcaaattctt
     9541 agcttgtgat ttagatacat taaatctctt agcaaagtca tttactagat tttttggatt
     9601 ttcacctcta ataattgatt gagttagctt agtatgtagt tcatttatta aagtaggtct
     9661 atgcttaccc caaattcttt cactaaagtt taatccatct gttgcccatg gtttagagat
     9721 aattttattt attctattag tatcaagact cattaaactc caaccaacgt ttactccttg
     9781 ttgaacatta aaagctgtat gatagtatcc acttgtataa atatctctca ttagtttatc
     9841 aataccatca agttcatttc catataaaac ttctacgtgt tgctgtattt gtaactttaa
     9901 agcttcaagc cttgttatat gaagtcttgc actagcattt tctaactcct tcatccactt
     9961 ttgatttata gcattttctt tgccatattt aatatactct tctacattcc acttaaattc
    10021 ttctaattct cttgtattta acagtttctt agcttctaat aaagatattc cttcattttc
    10081 agcaaatctg ttataccatg ataatatatc tttttctata ctattcatag ctaatttgta
    10141 tttcttttcc aattcaagat aatattttac acttttattg ttttgaactt cttctaattg
    10201 ttcaaatctt ttcctccaat aatctttatg tttcatctat aacaccctct tgattattag
    10261 taggaattaa atcatcatat tctttttggg tttcttcctg ttttttaagt ctttcaagtt
    10321 cgtcattaac atcttcaacc caaggatgat tactaacaat agtttcatct gatataattc
    10381 caattgattt agaagccata tctatttttt cagactcatt tattatcata gaatgattaa
    10441 atgttatttg aactgactta taatcatagc tcttactacc acttatcttt aaatactcac
    10501 acacaaacca taaaagctct ctaattgctt ttttaaactt cttttcagtc ttggaacatt
    10561 ttaaatcaag tagtgaatat aaaaatttaa gagctacacc cgatttgtca cctgtgtttt
    10621 gagattcggg attaactcct tgaccaaaaa taattatatt cttttctaat ctatcaagaa
    10681 gctccttttt ggcttcaact ggtatattta tctctagttt atcaactcca cctccaccat
    10741 ctactttaat tgatttatag tatcttatat tatctataaa ctcttgtaga cttgttcctg
    10801 gatactcttt taatacataa atgacctctt gcatttcatc taaattgtct gcaagtgtag
    10861 aaatgttatt atcatatata tcaattaaag acttatagaa agttaaatct gagacacact
    10921 tttcattatt tttaaaaggt ataaatggaa ctttacccca tccctgttct ttgttattta
    10981 ttctaaaatg accttcttgt atatcagtca tttttccata ttcatcatat aaaaattctt
    11041 gaataaaact attacctctt tcaataaagt aagttacgtc attttctgtg tagtactcaa
    11101 ctctttttat tttatttcca tctatatctt caatataata aaacctaata aatgcaacta
    11161 attccctctg tcttttacta tcccaaatag gaattgcttc ttcagctgga attattacat
    11221 atttaaactc accttttcta ttaatatatg gatgtaacca ttcaacccct ttattactag
    11281 cattgagata tagttctgtt attgtatcgt caaactcttc tcctagtaag tcatttaaaa
    11341 gcttagtgag attatcatca tctgcattaa atactatggg atttccgaca ctatagccta
    11401 ccttttggtc aactaaaagc ttatggtagt tgttaattgc tttattatta actttagtaa
    11461 aatcatcaac cttagctcca tctaagagat aatatcttct cttattgttt acatcagtat
    11521 taccataata gtattcttct ccttgtttat atttttctgg cctatgcttt aagatgtagt
    11581 gttctatgac ttttactagg ttaaaggtgc tctctttttt taactgaact tttattaaat
    11641 ctgtttcact tatataaata tttaacacct cctttacttt aagaagctta ttccattatt
    11701 tttaagctta ttatctatag aatatctaag agcagccatt gcatcatcca taaactcaac
    11761 tggttcatca agatataatc cagttctttc gtcttgtttc catttccatt gttgtatttc
    11821 ttttatggta ttagtgcaac taggatgtac atgtattctt aattgtttca aataatctat
    11881 ttgagcttta acacttcctg gtcctttttt aactccttta gctttatatc ctgcactctt
    11941 ccacatctta attctatctg gttcagcact atcacagtac ataaatagag tcttttctaa
    12001 acctctacta tttgcaattt ttatgatttc tgaggtatcc atttcatgta catatatttc
    12061 attacatata tataactctc catccttaaa gccaattcta agtattgcat ttgcatgatt
    12121 aaagccaaag tcttgtgaca gtctcatatt gtcaaagtat tcaaattctg tgggaaattc
    12181 atgtataaca taatttttaa gtattgctcc accagtttct ccccattctc caagaccata
    12241 tactttgtac ccttctgggt cttgctcttt tctcatttgc attcttctgt agtaagcttc
    12301 atctatgaat ctattttgta gataagtact atgatgagta aatatatcat catttttata
    12361 gtcaaaatac tttcttttta tccaatgagt agctgagact ggattaaatg taaatgtcat
    12421 ttgataatac aagttaggat tagttaaaat acctcttaaa cggtcatcta gtatatctat
    12481 gtcactttcc ataagttctg tagcttcttc acaccaaacc catgttaatt ttcctttcga
    12541 gaagttaatt gattttaatt tttctctttg ttttgcatca ttaactcctc tgaaaattat
    12601 agagttacca gtaactttac tcttaatttc taaaggattt aaagtagttt tccaatactt
    12661 atcagcttgt ttaccataaa tacgatttat agctcctgta agctctgcat acgttgaata
    12721 cttatgtgta gcttctgact ttctaactac taatagatta gctccttgat acttcttatc
    12781 tcctaacttt agtatatagt cttgtgctac attaacagat tttccactcc ctgctgaacc
    12841 tttcattgct ctgtatcttt ttttagtaaa attagcttcc ttgaaatctg gattaaaatt
    12901 tattctaact atcatttcta tcaccataat ctacacttat tttcaactca tcatctccaa
    12961 tatcatcttt atttaggtta gcaacctcac atttcaactt ctcaactctt gttttctgct
    13021 cctctgtagc caaattccaa tccttatgaa tcatttcatc atactgctta attaaactcc
    13081 ttaactcact catagctcta ctctgtgcat taagaaaaga tgcttgccta tcccatgcaa
    13141 attgaaattc atactctatc ttctcactat tttctgtgat ttcatgtttc tttaattcct
    13201 taatcatttc ctctttatct ttaacataca ttatcttctg tgctcttatt attgctgcgt
    13261 attgaattgt tatctgttcc caaagaatat caaatttatc ttttatagat atttcttgta
    13321 ttaattccct agtttcttca ggtaagtatt ttgagaagaa accaaacttt tcagcatttt
    13381 tattctcttt tggagcacca taaccaactg aatttttatt agaaaagggt gcaccccttt
    13441 tatttatagg tgcacccttc tttttttcac tagaccaatt gtatcttttt atccatgact
    13501 ttaaagtatt taaactaatg tcatactttg ctgatatttc cttttgtttc attcctttta
    13561 agtaatcttg ttttaccttt tctttgacat cttgcacatc accacctctt tatttgtttg
    13621 ttttgggaat aaaaaaacaa tcctatttaa taggattgtt tcaaatctag tctcctctat
    13681 ccttccagga taaatgaaac atacttatta ttaatgtagt tagagataaa attatatgaa
    13741 ctgtaaaaaa tattaaaaca tagaaaatta tattagaata atatcgagta agcattattg
    13801 tattgataaa acatattaca acaaccaaaa tctccattat tacagtataa tatgctttta
    13861 actgtatgaa ttgaaagtat gatatattaa atccatttac ctttctttct gtttttatat
    13921 cttttgcttc tgatattgat ttgttatatg tagtaaacaa taatgtaata atgcaaactc
    13981 caaaagctac taatagtcct gacaaagtta gtacatcttt gtttaaagaa attacaactt
    14041 ctttattaat tgacgaatta aaaaacatgt atgaaataat acatattaat actggtgtta
    14101 aaactaactc tactacttct ttagaattta tagccttgta ataattgagt aagatattta
    14161 ataccttttt atacatttta acacctcctt agcaatctat gcttcactca tagcaacttc
    14221 ttgatacatt aaattaaaaa aaactgaatt attctcaatt aaatcatcta tcaaattatt
    14281 catcttctca aaaatgtctt cactatctat atgattatct atatcaagag aaacctctat
    14341 ttctctacac aactttatac cctcagtatc caactttaat tttctcttat ttttctctcc
    14401 ctcaattatt atcctattta tttttttatt tctaaattcc tctcctttaa tatatttttc
    14461 gcaacatctt ttaacttcac taggtaaata tctagttcta aatgtgggct tatatatcaa
    14521 atcgttatgt cgtcttgaat tatttaaatt agaaaacaaa ttatcttcat cagttccaaa
    14581 cttctctcta tcaacattta aagttatttt tgaaacacca tctaaatcta atatttgttc
    14641 tataaaatct ttacttgcta tttgtgtaat attaattgat gtttttgaca tattattatc
    14701 aaatttatta ttttcaatca tatcctcata atatagatgt aagtttttac ttagttcaat
    14761 catgggaacc cccacaaaaa ctctttcaaa taatactact gctatattat tttccttcat
    14821 aaatttaata aataggtgct gcttattgct atctccttga tccatttttt tattaccctc
    14881 tacttcttta gtatgagcat ttataatatt tgtattctga ttgtatttag tatattcaag
    14941 tttcaccttg attattaaat catcctcgct acattttact tctgtaatcc aaaacttttt
    15001 atctttagta atattaattc catttttgtt ttctttacaa tattctttat ccttaaagaa
    15061 ggtctttatt acctctttta aaatttcttc atctgaatag acactcccat taattttatt
    15121 tataatatct attctataaa catcaatata tcttttttta aaattactca ttataccatt
    15181 attcctttca caaatattta taatgatata attctatata atcagtcaat atccttcaat
    15241 aatcattcga caaccacaaa ataagtctaa ataatacttt taccttattc ctgtctataa
    15301 tgtacaaata tatttcattt ttaattcatt acataaaaaa aaagaccctc tattaataca
    15361 gtcatttaaa tcatttcgat taattcttta atctttttat atacctcttt ataattcata
    15421 cctttatcta tcaacctagg taatttcata gatataactc tttcaagtac ttgtattaat
    15481 aaaaaaactc ttgtatttca tctgtaaaat cttttctgac cttttacctt ctgttcttat
    15541 atccagccat tcttgatctt tttctgtggc aatatagtaa ccatgtattc taatttgttt
    15601 aagtgctttt gtaacccact tcgtaaatag ttttgcttct gctttattac ttctaaatga
    15661 catattgtac acagcttctt ctgtaacaaa agtagtacca aagttaggca atttatcttt
    15721 aaagtttcta gtgtaggaat ctccgacagt agactcatta aatttctttt tatattctct
    15781 atctatattt cttaatgtat cacgaatatt tactatgcct agttcctctc ctacgtcatt
    15841 tgcattaaac caaacttctt ctccattttt tgaccatatc atccttacat ttttctcttg
    15901 taaaattttc aacatactac tacctcctaa attagttatt aataaagaat attcaatttg
    15961 aacaccctta ttttcgaccc ttcaatatga gagattaaaa ttgtataaaa aaagacctag
    16021 aaatcactct aagtcatgaa ttactcattt atcattctcc ttatttacaa aataaaaaag
    16081 actaagttgg ggtaacttag cctttttcaa gggggaatat attatacact tgtttcatac
    16141 taccattata acaggggtta aatatcatta aaatatcatc tttttatcat gtatttatca
    16201 aacagttaat ttcaagccat ctataccaaa taaatacact ccaagttctt ttaccatttc
    16261 attaatccaa cgtcttgatg taactactcc acaatttaat atctctgcta tttcttcata
    16321 tgttttttca tcacaatagt aaagctctaa agctctatac ttttcataag aatgtaaacg
    16381 ctcttgtctt aatttcaaag ttttcattgc tgaatctata tgtgctatca taataatagt
    16441 tcttgcttta cttttcttta tacttaaaat atacaactct tcattatcaa gcttacctaa
    16501 atcatcctct aagtaatcta catctttagc ttcacttacc gctgagtcta tatgtctctt
    16561 aaaatcatta taatgcttca tcaataatct agtattatat aaaacgcctt ttttcctatc
    16621 ttctctttct tcttttctta actctcttac aatttctttt atactttctt tatccactta
    16681 aatcactcct tcttgctctc ttcgaacaac ttctactgca atactcaact ttagatttat
    16741 ccaacttata aaatcttcta ccacaccaag cacatcttac tattttacct gggcttataa
    16801 gttccatctt ttctttttac tttcatacct tcactcccta tattctttta attttgcttt
    16861 tactgcttca agtaatgcac tttgcccttt atccttattc tctatagctt ccattacttg
    16921 ttcatctatt gtacctttac agattagatg atggataata acagtttccc tttgaccctg
    16981 tctataaagc cttgcattag cttgttgata cagttctaag ctccaagtta atccaaacca
    17041 aacaattata cttccacctg attgtaaatt aagtccatgt cctgtacttg ctggatgaca
    17101 aagtaataat tgtattcttc cattattcca atcatatata tcttttgagt tttctattgt
    17161 tcttggtttc aaactcttaa actctttcat taaacgatta taatcatgtt tatagttata
    17221 aaaaactata ataggcttac catttgaaac atctataatt tcttttaaag cttctaattt
    17281 ttctctatgg agttccttta catttttatc attatcataa attgctccat tagctaattg
    17341 taaaagttta tttgctgcta cagctgctga tgaagctgtt attatatctc tatctaattc
    17401 taatatcttc tctttttcta attctttgta atatttaagt attttactat caaggtgtat
    17461 ttcaatttta ttatctattt ttctaggcat attcaaataa tcttctgctt ttaagcttat
    17521 acaaatatct tttattttat tatgtattgc attttctgct ccatttttag gttgccaatt
    17581 atatatagct cctgtttgat aatttttttg tcctggctca aagtatcgtt ctttatatcc
    17641 tgtaatagtt ttacctagtc tttcacctct atcaagtaag tacatctgtg cccataagtc
    17701 tattaaacta tttggtgttg gtgtaccagt aagcccaact actcttttag ttaaaggcaa
    17761 tactttcttt aaacttttaa atcgttgtgc tttatttgat ttaaaagaac taagttcatc
    17821 tatcacaacc atatcaaatg gccattttct tttataaaaa tccactatcc agggtaccat
    17881 ttctctattt attatataaa tatctgagtc tgtacttaaa gctctcactc tatctaattt
    17941 actaccaaga actttagata cttttagatg ctttagatgt tcccattttt ttacttcact
    18001 actccaggta tctcttgcaa ctcttaaagg tgctataact aaaacttttg atatatcaaa
    18061 gtaatcatac attaattcac ttatagctgt tagagtacag actgttttac ctaatcccat
    18121 atctaatagt aaacctatat tattattatc tatagttttt ctaattgtgt attcttggta
    18181 tggatgtggt ttaaattcca tcccttaacc tccttgataa aatcatctat ctcttttaat
    18241 gtgcttacac acttaacttt aaatcctaat tctcttaatt ctcgcatttt atattgttga
    18301 agcttcctta gttttttacc tggtgcttta agttctacaa atataacatg tccttctggt
    18361 aataagacaa tcctatctgg cacacctgcc tcacctggtg aaataaactt catagccttt
    18421 ccacctaaca actcaatctc ttttttaagt cttttttcta tttttgattc taacaaaata
    18481 tcacttcctt aaaatttaag tgtcaacaat gtcaacagat tttctatata tatgtatata
    18541 tacatattag gcatacatat gtatatacgt atatgcctaa tattacttat actactttat
    18601 acttaatttt tgttgacatt gttgacatta atacctcaaa cattgtaatt actgcatctt
    18661 aggtgtcaac ataagcgtca acaaagccat tttttttgtt gacattgttg acacttttaa
    18721 aattttcaaa tttactctgt ttttaattct gttgacacta acttttacgt acataagctc
    18781 tctgcctacc atatattttc ccaaacctta aatttccatt atatgatatc caatcatcta
    18841 atccttttaa tatatcatta atttctcttg ataagatagg cgtgagttgt ttaggttctc
    18901 cattaaatag ctcaacccat atctccataa cacacgtttt ttctcttaat attgttcctt
    18961 cttttaaatc accaaaatct gaaccatgaa tatattctct cttttctgaa atacttaaat
    19021 cataccaatt ctcagtaata ggtttattta gatactcttc gataattcca gatttagcat
    19081 tttcttctga gtgagttctt tgttgtcttt cagcttcttt tttctcctca tctgatagat
    19141 atagctgttc attggcttta cataattcta ctgcttctgc ccaaatttga tttctttcat
    19201 tatcaagttg accattaaat atactcttgt taaccttctt tactcctgta tctattggcc
    19261 aaaaccttct attcccggtc ttatctctta aaaattcttt atcattagtt gttcctataa
    19321 ctacacattg acgtaaaaac cttgatgttc tcttcccata tgcgacccta tatatatcct
    19381 ctgatttgct tagaaaatgc ttaactgcct caatatctgc ttttttagta gccatcattt
    19441 cacccatttc cagcaaccat actccttgta attgttcata cgcttcttta ccttgtacag
    19501 tagttaaact atcagaatac cagtctccac caagtttttt aataaaagta ctttttccca
    19561 ttccttgagg accagataaa accatcatat tatcaaactt tattcctgga ttaaatactc
    19621 ttgctactgc tgctactaaa acttttctta ttatagtcct tgtatagtgg ttatcttctg
    19681 cacctaaata gtcaattaaa agtgtatcca ctctcttaat gccatcccac tttaaagaat
    19741 ttaaataatc ttttatagga tgaaaagtat tattttcaaa agcaattatt agagcatcat
    19801 ttacttttga tggtgatgag atattgtaaa ttgtttctat atgatgtcta agcccagaat
    19861 catcactatc attccaatca tttaatttat catcttttct ccaaggtaac ttacctaaaa
    19921 cgacagctct atttgaaaat tcattataag ctatttttcc tttcaaatat gggtcatttt
    19981 ctataaacat taagatattg tttgttgttt tcttataact tcccttattg tcataatcta
    20041 acctagttaa ccattcatca tcttcaaaat ctatatcacc aaaatcatcc ttagctttat
    20101 caaggttttc tcttcctata gtctttcgta ctttagtgtc actgcttgca aattcgctca
    20161 ttcgagtaaa tgaaggtaat ctatttacag gcgtttcggg tttagcatct tcatcaagtt
    20221 caccaaattt atgtatccta actaaatcaa aagcattgca taaaattccg cttgctgggt
    20281 ctgtaccatg atgactatat gaaaacttgt catcataaat aactactcca ccacttgtac
    20341 taccttctga atatgtatac ctggtttcat caataccagg aatatatact tcatttaaga
    20401 aattttctat agtttctttt atactatagg acctgcaaaa tgcacctata attccagcct
    20461 tttcaatagg gtcttgttgt ttttttaatt gtgtattaaa cttttgcctc tccctcgaac
    20521 tttctggcca atagcttaca tctgtccagt ctaaatataa atctagtatt tcatttgggt
    20581 ttaaaaactc tccatcttga aatttaaata tatagtctcc atcaattgaa gtacttggaa
    20641 aatacataag tctatgaggt tgatatgtag tatcatcaaa catatctatt cctatagtat
    20701 ctgctatcat cctggaaatt gcttgatatt cttctggtag tactggtcta gttaaaggta
    20761 ttacaagtct atatctggga ttattctcag tatgtgagtg cgtagaatac ataagacaag
    20821 cataatcatt taataatgtt atatcctccc atatatcttt attcgcataa tctatatcta
    20881 aagttataat acttctattt gcaatattct ctgcttttcg tcttccattt tttaagcttc
    20941 cacctacaaa tccaccaaca tctttaactc tatctttttc agtctttgac atcttcctat
    21001 attctgtata tgtttcttga gttcttaatg ttttacttaa tctatttaca agttcggacc
    21061 ataaaatact tttatttttc caatgtgttt ctaatttatt ttttcctatg gccagcatga
    21121 gttggccatc atgtcttaca tttatgtgtt caatttcact ggcctttata tccataaatc
    21181 aatcacctag tctttcttat aataatcaca ttcatatcca tctgctttaa gaggaagacc
    21241 tttagcccaa gatatttctt ttcccataat actgttaact tcttctaaag aacctccttt
    21301 tttatctaca tcaattacaa gctcatcatg tacatgcatt acaatgctat aacctgcatc
    21361 tgtcacatta aacatagcct ctcttaagca atctctagct gtagcttgaa caatattctc
    21421 aactaactta ggtccataag tatctattct tttccattgt ttacttgtct gttccatacc
    21481 ttcatatgtt atcttatccc cactaaatgt agtatgaggc tctatcttag gtcttagata
    21541 tgataatctt ctaccacttg gtagttctat aaataaaact cctggattat aaataaattt
    21601 aatcccatgt tggagtttta ctatagtcct atcttttatg gcttttttag ctgccttatc
    21661 tacatcccac caaaatttag ttatatttgg attagcattt ctccatgttg ttacaagagg
    21721 ttgaagctcc tcttcatcaa gacccatttt tatagccccc attgatgtta aagctcctat
    21781 gcttccccca tatccaagag ctagctctga aatcttacct ttttgtctaa gtgggtcacc
    21841 ttttttaata ctttctattg gaactttaaa catatgacta gcactagctt catatatttt
    21901 cccgtgagaa ttaaatacat ctaatctcca cttctcacca gcgaaccaag ctataaccct
    21961 agcttctatt gcactaaaat ctgatactat aaatctatga ccttcacttg gtataaaagc
    22021 tgtccttatc aattgactta agacatctgg tacactatca tataaaagct ctattaaatc
    22081 aaaatctcct tcttttaata ggtttctagc taggtctaaa tcctctatat gattttgtgg
    22141 taaattctgt acttgtacta atcttcctgc ccatctacca gtcctattag ccccataaaa
    22201 ctgtagtaga cctcttactc tattgtcatt acctttagct aatttcatag cctcatattt
    22261 ctttatagaa gttttggaca ttaattttct aagttctaaa attctaacta cattttcatc
    22321 atcaacttgt tttaatattt ctggaatact ttcttttgtt aggcttgtaa tctcaaagcc
    22381 aactttatca cttaaccatt tttttaattg agctggacta tttggattat ttagaccagt
    22441 tattttaatt gcttcttttg taagtttttc agtatatctt ttatcacatt ctattgcatt
    22501 ctctattaac tctgtatcaa ctttaatacc agtatcatta attctctggt ctaaatacca
    22561 taatttaatt tctctttcgg tagtcttata cttactaagt ttgtttctta tttctctttc
    22621 aactacaaca tcttgtttac aatattcttt aaatttattc catttttcca tatcatgtat
    22681 tggtaaattt ctagttcttc ccttgttaac ttttgtagcc ttacaaggtt tacagaaata
    22741 ttgtattaat gctttacctt ctttcatttt ctgtttatct tcattaaact ttaaagcctt
    22801 agacacacta tctaaacttc ctggaagtcc tagtgttaac gcctttatca ttgtacatga
    22861 ccactcattt ggttttaaat taatatttaa aaatttactt attgctgttc tttcaaaatt
    22921 agcattaaat gctgatttta taactttatt atcatttaaa gcttctatta cttcttttgg
    22981 taactcttca tcatttacta aatcaataac ttttacctct tcattatcaa aagcataggc
    23041 aaataacagt atctcaaaat tagcagagtc tacatatctg tagactccaa ctttttttat
    23101 atctaaatca ctatatgttt ctatatcaat tgataaggtc ctcattaact taagaagtcc
    23161 tcttcttcat cttcatattc aaagtcatct gagaaatctg cttctgcact agctctagct
    23221 cctccaagta cttctccatc tgctaacttt tgaacatttt gtaagccaca acctattcct
    23281 ttatttcctg cactattata tgggaaaaag tttatactaa ctcttccata acaaccacta
    23341 tatacctctg tattatctaa tatttcattt aagtctttat caactattcc tggcttttgt
    23401 gtactatttg catttaaaaa atacattcct acatattcct ctgcttcatc agctctttct
    23461 gcatctccat cacgaagagg tgtttttaaa ttacctggta attttccacc ccatttagaa
    23521 gttttacctt gttctttagc tgcatcaata gccttcttta ttcttcctaa agtcacctta
    23581 tctgattttg gtattaaaat acaaactgaa tacttgggct ctgcaccttc taccattgct
    23641 ctgcttttaa agatattgca ataacttaat cttacctttc ctgttactac ctttgttgat
    23701 tgtactgaat tactcataat tttcttcctc ctaatatttt tatatttggt atattgattt
    23761 tatatttgaa gttatttctc caaatttatc taaatctcta acttgcttac taaacaattt
    23821 aattcttcca caaataactg ttgaagaaac atttaataac tctcctatat cagatatctt
    23881 tttatcttgt aaaatccaat ctactatttt atcaatatct tttaaatatg actttcttgc
    23941 aatttttctt atataatcaa catcatagtt aaattgaatt tcataaaatc catgtatatc
    24001 ttttaatggt cttttactat cttcaaatac acttacatct aaaccttcta tagagtttct
    24061 ataatgtttt tcgccataac gcaaagcttt aaacatttct attcttatgt aagaaacagc
    24121 cactgtagaa aacttgcctc tgttactatc ataatttaat gctgctttat ataacccaat
    24181 acatccttct tgataaaact cttcatataa gtatggatgt tgctctacat aagacttaaa
    24241 aaatctattt atactaaaat gtacaagatt caaattattt tcaactagtt tagttatttc
    24301 ttgattactc ataaaattat atcccctttt aaatcttttt atagctacta ggaattaaat
    24361 atttttaatt atcctttgct cttactgata caaagcatac tggactaact tttatatgtt
    24421 ctggtatata cactctatca agatgattgt catttacttc gaggaaagtt gacatcactt
    24481 tatcctttat taaactttct cttttaaaat ccatcactat aacttttgca ttttcttcag
    24541 ttgagttata ttctgtaata tttagtctct ttaacatatc tagtaaatta tctttttcat
    24601 aatctaaatc tttttcgata ctcttttttc tatcctttaa atctaaaatt atacttatgc
    24661 tttcatctaa tttatctttt atacctgcat ctaatggcat gtaatatccc ccatttatat
    24721 ttcaaaatct tttttcgctg aatctatact attaatttct gaccttttat cactttctac
    24781 tactaaagta gcttttccaa ctggttttat aattaaatca cttagtagtt tagcaaaact
    24841 ctttttacct atagctttct ccatatcact aattcctttt aaggtccttg gcttataaat
    24901 tttttcttca tcataatctg aatttaataa aaccttagca acttcttgct catctacata
    24961 ctttctatta cttctgcctt ctacaagctt atatcctgga tatttaacac catgcttttc
    25021 agcttgttct agtgcataac tttgaacatc ctttaaccaa tcttgtatat tcttagcaaa
    25081 acctaaaata tctgctatct catatttatt aagagcaaat gtgtcagcaa aatcatattt
    25141 tctagctagt ttaaggttat cctcagctct ttttctgcaa tcatttttag ctctacaaaa
    25201 tccacaatga ctgccactta caaactctcc ttcaccatta aaagccattt gagccttctt
    25261 cttaacatta tctgcccatg taagtaattt agtaacttct atttcttcac ttgatatgtt
    25321 atcaagtcta ggttgaatta ttgttgtttt aattaaatca atatcgtata gcatttcaaa
    25381 ctgattatat gcccctaaac catatagtct aagttgagga ttctctatag ctgaaacttc
    25441 taaacctttt ccatacttta ggtctataac ttgaagtata ccatcagata ttacaacaac
    25501 gtctcctgtt ccaaatcctt ctggaaccca ctcactaaaa tcgagtcttt cttctaacat
    25561 cactattaca tcatcacata tagctttact atcatttact aattcaacta cattctcaac
    25621 ataagattgt atatagtcct ccatttcaga gttataatac tcacttttct gtatcttttt
    25681 aattcttgca ttatatgcct ttttacttat tttttcatac tctagcatta atttaacttc
    25741 tgctaattca tgtgcaactg ttccttcttc tgcatatata ctagttaatg gtggatagtt
    25801 ttcttctaat tttatactag gagtgcagtg aagccatcta tgggctccac tcgcactaag
    25861 tcttgcatgt tgtaatggca tttatgtacc ccctataaac tttctaattt gttcataaaa
    25921 gctgaataat cttcttcttt tacttcactt aacttactag ctccaaattc accaaataac
    25981 tccttaagct tatccttctt acctgcctta cttacttgtg ctgccttagt tcttacctct
    26041 tcttttgtgt atttaacttc actagttgta ttttcattat tcttttctat ttcttccttt
    26101 gcttctgcta tttcttcttt aacgtcttct tttacttcta cctttttaat cttttcagct
    26161 tttttaactt caactttctt ctcttctttc atatctgtta tatttatagg ttctatctgc
    26221 attgcttttc ctagattaag acctcctaaa gcattcgcca ctactaatag tgcatttgta
    26281 aattctggtg cttctacttt aacttttaca tttacattaa cttcaaccat tttaaatatc
    26341 tcctttttgt gttatacttt acttgtgtta tattttattt tttatttttt tgtgtgttgg
    26401 ttattttacc aacacttttt tatttaatat tccaaccgat attttcttac cagttttaat
    26461 atctttaaat actatatctg ctataacttt tccatctttt ttaagagtta ctacattctt
    26521 attattagta tcaagactca gcaatttcat ccccccactc tactgctttt aaaagttcat
    26581 ccaaattttt accttgattt ctttcaataa aatcatcaac ttcatatctt gaaatttttc
    26641 taccatcacc tctaactagt gattttatca aacctgtgct tactaaccta cgcataaaag
    26701 ctgtatctaa ctttaaaatt ccccttgctt cttctactgt tattagataa tttggataac
    26761 ctctttttat taaaacaact atatctttag gctccagtac ctttaccttt tgttcaatag
    26821 tttgactctt aactctatct gtctcttgtt tacttatctc tatttctatt aaacttttta
    26881 aactatcact aaattgtttt gctatactct cagaaatatc catgtgaaaa ttctctcccc
    26941 tctttttaat tatttatctt ccaactaatt cgtctaatgt aatatctaaa taatcagcta
    27001 attttattag agtatctata gttggattag aacttttatt tctcagtaat ttatataaac
    27061 cacttacatc tatattcaat tcttttgcca atttataagg ttttatatct ctttttctta
    27121 gcatttgctt tatattgtcg cctattaaca ttgtattcac ctcagttcta catggtataa
    27181 tataaacatg gaataaattc caaaattttc aaaaggatgg tgtattatgc agttttcaaa
    27241 caatatatct aaagaattac aagaaagtat aagaaagaca gtctctgata cttctaacac
    27301 agagtatttt tactatgctg aatttcaata taaaattatt cttaaatcta tagaggaatt
    27361 tgaaaaagaa cttgatgatg aacatgaaat tgcactaaaa ttgactaact ttggaaaaga
    27421 tgtcttaatg attgttgaag aagtaggtta tcataaccct tgtctaattc actactacgg
    27481 catagtaaat ggagtttatt ctgaaattct tcaacacaca tcacagataa actttatgat
    27541 aacttcagta aagaaaactg acccttcaaa acctgctaga agaataggtt ttatattgta
    27601 attatcattc ttattttctg attctttctt tagtaatttc actaattttt cttttttcac
    27661 ttttattcat ctcatgtaaa tacaaaacta tacattggta catataatct ataaaatcac
    27721 tatttgattg agctgttatc tgactaaata actgtgcttg caattgattt tcaagcatgg
    27781 ttattctttt ctctatttct tctattttct tattttccaa ttcaatctct cctttctaaa
    27841 atattctgta tttagttttc aaagtactaa cctaatattg aagtttgata actatatcct
    27901 ttttctatat ctggtaatat gttatttgtt ttcaataaat catataaaaa caatcttccc
    27961 ttttgagtcc acttagttgt catttttaca tcaggcattc cattacttct agttatgtct
    28021 attgtttctg aatgagtgta tcccttccct tgatgttgtt tatataaaag ccattgtcca
    28081 ctttgtttat attgaatccc tctttcatga agtattttat tcatttcttt tccactcatt
    28141 ccatagtctt ttgctatttg agttattgtg acaagtcctt tattttttaa tatcatatct
    28201 gtataatctg cctttggttt tagttctttt attacttggt ctttcatttt actttctagt
    28261 tgtaactgct catttacttc tacttgctct ataagatgtt gtaacgcttc tttatatgta
    28321 gttggtagtt tagtttgcaa atctatcata ttatatgtcc cagtactacg tatacttggt
    28381 aatatctcat tagttaccca acgtttaaat aattttgcac ttggcaactt agaacttaat
    28441 attaaagaat aaagaccact ttcattaatt actttcatat tttgattacc accaggagtc
    28501 ggtatttcac ctactccttt atcttcatca tcaacatgtc ttttcaaagc atcagatgta
    28561 tctttatatc ctaattgttc tgcaatatct tttccaacaa accaaaattc tccatttagt
    28621 tcgattactc ttatttctcc aaaagtctta tttttgaata tttgtaaatt attcataatt
    28681 tctttcctcc ttatgattgt ttttgtgaac aataaatttt aatttatgtt aacctttttt
    28741 ctaaaaaaag ttcatccatt gttttattta aaatatagct aatcattatc atttcttttt
    28801 ccgtaaaatt tctttttcca ttttctttta gattatatgt tgattttgct attcctatct
    28861 tatatgctag ttcttcttgg gtatatcctg cttctattcg accagctttt aaacttcttt
    28921 tcttcatgtt cttcaccttc caatgtttta tttttgtgaa cttcatactt ttattatatt
    28981 cacacaaaca aaacttgtca atacataagt ttattttttt gttaactttt ttattttttt
    29041 ctaaacataa gtttacgttt ctgtaaactt atagtatcat ttaattattg ggaggtgttg
    29101 tttgtggcaa cttttggaga acgtttcaaa caattacgag aagagaaaaa acttactcaa
    29161 gatgaactag tttcaaaatt caataaagtt tactttacaa gttttaataa atcaacaata
    29221 tcgcaatatg aaaataataa aagaaaacca gaaataaata ttctagaaaa ttgggcagat
    29281 ttttttgatg tatcaattga ttatcttttg ggtagaactc ttgttagaaa tcacatcgat
    29341 actgtagccg cacacagagt taatcctcac aaagatttac cagaggaagc tcaagaacaa
    29401 ctcaacgatt atattgaatt tttaatgaat aaatataaaa aataaggatt gaatctatgt
    29461 taatattaaa aaaattgaga actgaaaaag gaatttcttt agataaatta agcacagact
    29521 taaatataaa taaatccact ttatccagaa ttgaaaatgg gttaagagaa ccaaaagaat
    29581 catttattaa agattgctca gactattttg gagtatctac agattactta attggaaaaa
    29641 ttaacataga tgattcaaat aaattaacaa aatgcttaaa ttcaactttt ccaataaggt
    29701 taaaagaatt aagaaagaaa aaagaattaa ctcaagcaga attatcaaaa ttacttaatt
    29761 gttcactaag caaaattgct atgttagaaa ctagcaaaag agaacctgtt aaagaagact
    29821 tgttaagaat ttcagaattt tttaatgtgt cagttgatta tctacttggt aaaacctcaa
    29881 ttgaaaatta cataactact gatgaaatat ccaaaataat taaatcttat gaaagtttac
    29941 ctaaagaagc gcaagaacat attaatagct acatagagtt tttagttgat agatataaaa
    30001 aatgaatatt tagagcagtt cactcctgct ctttatatat aaaaaagtaa cacatacatt
    30061 ctttttatag ggggatttca atgaacaaac tagacgcact tttagactta gcaaataatg
    30121 aagagataga aatttactac actgacaaaa tagcagatga cataaaagga ttgtatataa
    30181 acagacaagg actaaaaatt atatcattac ttaattcatt aaaacaaaac aatgctaaac
    30241 taatagaaat cttagcagaa gaattaggac atcattttac cagtgttgga aactatgtat
    30301 cttcaaaaaa cagttacaaa aataaaatct tgatagacaa aactgaaaac aaagcactaa
    30361 aatgggcatg tgaatttctt ataacagaag aagaaataat acatgttatt aattcacacg
    30421 ctacaagtgt atacgaaata gctgaagaat tacaagttag catcaacttc ttactaaaaa
    30481 gattagaatt tctatcaaaa aagaaaagca tgttggactt aggaaataat agatttttag
    30541 tattaactaa tttgccaaat ttctacatat atgaggatat tttttaaact catttattct
    30601 acttttatag attttttact taataa
//
LOCUS       DAMJVX010000027        29913 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMJVX010000027 DAMJVX010000000
VERSION     DAMJVX010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29913
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.029"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            124..264
                     /locus_tag="PC407_001203"
     CDS             124..264
                     /locus_tag="PC407_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496660.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(439..759)
                     /locus_tag="PC407_001204"
     CDS             complement(439..759)
                     /locus_tag="PC407_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2496661.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(879..1616)
                     /locus_tag="PC407_001205"
     CDS             complement(879..1616)
                     /locus_tag="PC407_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDF2496662.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1831..3654
                     /gene="acd"
                     /locus_tag="PC407_001206"
     CDS             1831..3654
                     /gene="acd"
                     /locus_tag="PC407_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDF2496663.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYIS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3869..8167
                     /locus_tag="PC407_001207"
     CDS             3869..8167
                     /locus_tag="PC407_001207"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDF2496664.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEILPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8371..8832
                     /locus_tag="PC407_001208"
     CDS             8371..8832
                     /locus_tag="PC407_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDF2496665.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8862..10016
                     /gene="nusA"
                     /locus_tag="PC407_001209"
     CDS             8862..10016
                     /gene="nusA"
                     /locus_tag="PC407_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDF2496666.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVKSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELGTSEDNFSEEE"
     gene            10023..10304
                     /locus_tag="PC407_001210"
     CDS             10023..10304
                     /locus_tag="PC407_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDF2496667.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10294..10605
                     /locus_tag="PC407_001211"
     CDS             10294..10605
                     /locus_tag="PC407_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDF2496668.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10627..12567
                     /gene="infB"
                     /locus_tag="PC407_001212"
     CDS             10627..12567
                     /gene="infB"
                     /locus_tag="PC407_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDF2496669.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12610..12990
                     /gene="rbfA"
                     /locus_tag="PC407_001213"
     CDS             12610..12990
                     /gene="rbfA"
                     /locus_tag="PC407_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDF2496670.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDNLIKRVNEKNAQQNEDENYDE"
     gene            12971..13954
                     /locus_tag="PC407_001214"
     CDS             12971..13954
                     /locus_tag="PC407_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDF2496671.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNVGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEGVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQTKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSDYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            13967..14872
                     /gene="truB"
                     /locus_tag="PC407_001215"
     CDS             13967..14872
                     /gene="truB"
                     /locus_tag="PC407_001215"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDF2496672.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEVVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            15003..15932
                     /locus_tag="PC407_001216"
     CDS             15003..15932
                     /locus_tag="PC407_001216"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDF2496673.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEYAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            16036..16293
                     /gene="rpsO"
                     /locus_tag="PC407_001217"
     CDS             16036..16293
                     /gene="rpsO"
                     /locus_tag="PC407_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDF2496674.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            16533..17378
                     /locus_tag="PC407_001218"
     CDS             16533..17378
                     /locus_tag="PC407_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2496675.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            17503..19614
                     /gene="pnp"
                     /locus_tag="PC407_001219"
     CDS             17503..19614
                     /gene="pnp"
                     /locus_tag="PC407_001219"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDF2496676.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            19880..20623
                     /locus_tag="PC407_001220"
     CDS             19880..20623
                     /locus_tag="PC407_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2496677.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            20781..22028
                     /locus_tag="PC407_001221"
     CDS             20781..22028
                     /locus_tag="PC407_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2496678.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAICLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            22063..22308
                     /locus_tag="PC407_001222"
     CDS             22063..22308
                     /locus_tag="PC407_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2496679.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            22318..23541
                     /gene="dapG"
                     /locus_tag="PC407_001223"
     CDS             22318..23541
                     /gene="dapG"
                     /locus_tag="PC407_001223"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2496680.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEENIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            23654..24388
                     /locus_tag="PC407_001224"
     CDS             23654..24388
                     /locus_tag="PC407_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HDF2496681.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            24511..26922
                     /locus_tag="PC407_001225"
     CDS             24511..26922
                     /locus_tag="PC407_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDF2496682.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKSNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKSSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            26925..27983
                     /gene="mnmH"
                     /locus_tag="PC407_001226"
     CDS             26925..27983
                     /gene="mnmH"
                     /locus_tag="PC407_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDF2496683.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESVFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            27977..29311
                     /gene="rimO"
                     /locus_tag="PC407_001227"
     CDS             27977..29311
                     /gene="rimO"
                     /locus_tag="PC407_001227"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDF2496684.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFSELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            29298..29840
                     /gene="pgsA"
                     /locus_tag="PC407_001228"
     CDS             29298..29840
                     /gene="pgsA"
                     /locus_tag="PC407_001228"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDF2496685.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
BASE COUNT        12070 a         3182 c         5691 g         8970 t
ORIGIN      
        1 atcctaatta tgtcaaaaaa taaaaaaatt tttatttatt agagtttttg gaaataataa
       61 taacagatat tataattaat taacagtttt tgtaagtatt tgacaataga gagggtgtaa
      121 caaatgggaa taatagagtc agcatcaaaa ttggctgaga tggttcattt attggctgtt
      181 gaaaagggga taactgatat agaagcatgg gatgaagctg tgaaagaata cagcaaaatt
      241 tatgaagaaa gaagaaatga atagaatgat aaaaaatgaa aacttgatga taaaaaataa
      301 ctttatttaa agaaattcaa tttttataga aatattaaaa ataatataaa taaaaagatg
      361 gtatttcaaa tgtaagtaaa aaatcatttt gggataccat ccttattatt tattttaata
      421 atatataagt aataaatctt acttatccat attttttatg taacctcttt tttctagctt
      481 ttttatatgc tccttacgta tttcatcaag attaatacca tattgttctt ctagcttaaa
      541 catcatagta acacatgtct gagctacatc cattagttcc ttagcagttt tctttataat
      601 atcaacttca tctaaatcat tattttctcc acttagattt ctgaatttac ctatacattc
      661 agctaattca ccttgttctt cacccatttt gataaatgta gattctagtg ttgggtctaa
      721 attattaagc tctggtaaag atatagtttt tagtttcata aaagctcctt tcaagttatt
      781 cgtatttata ctaaaacatc ataacaaaaa taatactaaa aaaaaacttt aaattcaaca
      841 taaaaactta catatataat aaaaatcgtg aaataaatct atatttgtct taatacagcc
      901 tcatataaaa cttctccaat aggtttattt attacaagag aagctgactt atcatagact
      961 gtatttgctt tattaataag agcaatataa tcacctttgt aataatctat aaaacttgct
     1021 gctgggtaca cagctaatga tgtacctcct ataatcaata aatcagcatt acttatagca
     1081 gaaattgttt tagtaatgac atcactgtca agagcttcct cataaagtac tacatctggt
     1141 tttactatag aaccacagtt gtcacaataa ggaatattac ctcccagatt taacatagat
     1201 tctaagtcaa aaaacttacc acatttagta caataatttc tatgtacaga accatgaagt
     1261 tctagcacat ttttacttcc agccatttga tgaagtccat caatattttg agttatgaca
     1321 gctttaagct tacccatttc ctcgagtttt gctaaagcta tatgagcatt gttgggtttt
     1381 gcattaggat atattaattt atccttataa aattcaaaaa attcttctgg atatctaaca
     1441 aaaaaagtat gtgaaactaa ttgttcagca gtaaattgtt tattgagttt ttggctaaat
     1501 aatccagtag aacttctaaa gtctggaata ttagattcag ttgaaacacc tgctccacca
     1561 aaaaatacaa tattattgtg attagctatt aagtctttta agctatttgc atccatagtt
     1621 acacctccaa attattatat ataaattgta ctacatatta ggtcttttat gttttattaa
     1681 tttaaataaa tatgtagttt aataagttaa ataaagggaa tataaataaa atgtatacag
     1741 agtataaata gacaatattt actaaaataa caagttgtcg tataattata ttaaaattgt
     1801 tttagtgtat aagaagggcg ggggaagtac gtgaagaaaa aagcagcttt agcaacattg
     1861 gctatgttac ccttaggtgt ggtaaatgca catgctgatg gagatatagg tatagtgact
     1921 ataaattatt taaatgtaag aaatgagcca actgccgaaa gtagcatagc ctttgttgct
     1981 aaaaaagatg ataaagtttt gattaaagac tcttctaatg gatggtataa aataaaagct
     2041 gaatctggac aagaaggttg ggcttcatca aaatatatag caaaatcaaa tagtgactct
     2101 ttaagaacgt ctacaaataa agaaaaacaa gttatttcaa atagtttaaa tatgagaaat
     2161 ggagcaggta caagttacag agtaataact gtactaaaaa aaggtcaaaa agtagaagta
     2221 atatcagaga gtaatggatg gtctaaaatc aaatatgatg gaagactagg atatgtatct
     2281 agctcttatt taggagacgt ttcaaattca actaataaat caaaaactaa acaagtgaat
     2341 acaacttcac taaatgtaag gagtggacca aatacaagtt atggtttgtt aggcaagttg
     2401 ccaaaaggaa gtaaagtaga agtaatatca gaaagtaatg gatggtccaa aataaagtat
     2461 aatggaaaag atgcctatat atctagtatg tacttatcgg atgtaagtca aagtaattca
     2521 gacaattcta gtcaaagcaa tgataaaaag aatactgata aggttgtaaa tacagcttct
     2581 ttaaatgtaa gaagtggacc aggttctaca tatagtaagt tgggaaaagt ttataaagga
     2641 agtaaagtaa ctgtactatc agaaagtagt ggatgggcta agattaattt taacaataaa
     2701 gaagcatttg tagtaggcaa ttatttatct acttcagcag atacttcaaa taataactca
     2761 aatagtaact ctgataacag ttctaatagt aatggcaata attcttcatc atcaggtcaa
     2821 gtaaatggta tgtcaggtat aagtggggct aaaattgatt ataaatcttt gagttatact
     2881 ttagaatctc atataagtaa acaagttgag aaagcagcat caggaggaaa tgtgatagct
     2941 ccaagtaata ggaaaagcac tccaagtcct gaatttagta ctttttcagc tcaaagaaca
     3001 agctcatttg taaatgcaag ttcaagtgat atagaatatt atttaaatcc taaaaatttt
     3061 acaaatacta ctaaaggtat gatgcagttt ttaaagatta acagttatag agatggtatt
     3121 tcagaatcaa gtcttaactc gtatcttaat ggtttatctt ctagtgtatt taaaaaccag
     3181 ggagcagcct ttataaatgc tgccaagaag tataatatag atgttgtata tctagtatct
     3241 catgctatgt gggaaactgc ttatggtaag tctacacttg cccaaggtca aacattaaca
     3301 tcttataagg gacaacctct tagtaagcca gttaaagtat acaatttttt tggtatagga
     3361 gcaatagata aaagtgctaa tgtttcaggt gcagaagcag cgtattccaa tggctggact
     3421 agtgtagaag ctacaataga tggctctgca aagtggatat cacaaaatta cgtaaatagt
     3481 tctaagtaca atcagaatac tatctacaag atgaagtgga attatgatta tacatggcat
     3541 cagtatgcaa ctgatgtaaa ctgggctaat ggaatttctg gaattatgga aaatttaatt
     3601 ggtctttatg gaggaggaag tagtttggtt tttgaagttc ctcaatataa gtaatacata
     3661 aataaaaaat atagactatg ggtagttttc attttattta aattatctat agtctttttg
     3721 tatataattt ataatctgta aatttaatgt taacaaaact tttatgttat aatattaatt
     3781 ctaagtaaat ataagtgagt aataggtaga aatagataaa aagtggtaat aatatatata
     3841 aagataattt tgagaaagga ggatatatat ggaaagtatt aaggaatatt tggataagtt
     3901 agaaattaat aatagtggtc ttggaaagca acttaaagaa gtttatataa atagagtagt
     3961 ttattttaag gaagataaga tagtttattt ttatctaact tctaaagata ttgtttctca
     4021 tgaactttta gataaattta aagaagaatt gatgtataaa ctcgattatt ttaaagatat
     4081 gaggatgaag ataagattta ctggtcttga aagaaaatcc aataaagatg ttataaaaaa
     4141 atattggaat aatatattgt atatattaaa gtatctttgt ccatctattg cagggtggta
     4201 taaacaagta gaatttcttt gtttagaaga agaattaaaa ataaaacttc caaaaggtat
     4261 attttatgag cgattaatga aaaaaaatgt aacttatgta cttaaaaccg tgcttagcga
     4321 agagttggga ttagatttaa atataacaat agaaaaagct gttgatgaaa aggtaaataa
     4381 agaaagactc ataagaatca atgatagaga aatggaagaa aaaattaggg ctttagaaat
     4441 tggaaaagtt aataactgtg aagaaaatga agaggaaagc tatgtcatta agtctgatgt
     4501 agatgaaaat ttaatctatg gagataatgc aaatgctatg attgaaaata tagttgaatt
     4561 aaatgcatct tctggaacag ttgcagttgt tggagatata tttgatgttg acactaaaga
     4621 acttaaaaac ggaaaaatac ttatgatagc ttcaattaca gattatacta gctcaataag
     4681 ctgtaaactt tttttaacag atacaaataa ggatggagta cttgaaaatg ttaaaaaggg
     4741 agcatactta aaaatcaagg gtgacatagt ttatgataca tatcaaagag aaattagtat
     4801 gatgattagt gggataagaa aagaaactag aatagagaga gaagataaat cagaggaaaa
     4861 aagggtagaa ctccatgcac atacacaaat gtcttctatg gatgctattt gttctgttaa
     4921 aaagcttgta gagcgtgcag ccaaatgggg tcattctgct attgctataa cagaccatgg
     4981 tgttgtacaa ggatttccag atgctatgaa tgcaggtaag tcaaataata taaaaatact
     5041 ttatggagta gaagggtact tggttgaaga tgattctctc attatagagg atgcaaatga
     5101 taaagaactt tcgcagacat ttgttgtatt tgacatagag acaactggtt tttctaatac
     5161 aaatgataaa ataactgaga ttggtgctgt aaaaatagaa aactttaaga ttgtagatag
     5221 atttagtgag ttaataaatc ctgaaaaaga tatttcctat aagatacagg aattgacagg
     5281 aataactaat gaattaatta aagacaagcc aaccattgaa gaaattcttc ctaagtttat
     5341 ggaatttgtt ggagatagtg tattagtagc acataatgca gagtttgata ccggatttat
     5401 atcgcaaaaa tgtagggaac aaggcttgtt atataataac aaaaaagtag atacacttat
     5461 gttagcaaga gtaatgttgc caaacttaaa aagacataga ttaaatgtcg ttgcaaaaga
     5521 acttggtatt cctcttttaa atcaccatag agcagtggat gatgctgagg ctacagcact
     5581 tatatttaat aagtttttgc agatgcttac agaaaaaggt gcaaaaactc taagtgatgt
     5641 aaacaatata cttggaaaga ttgattacac aaagcttagc acaaatcata taaccttgat
     5701 agcaaaaaat tctataggta taaaaaattt atataaaata atttcagatg cacatgtaaa
     5761 tcattttttt agagcaccaa gaatccttag aagtgtatta aatgaatata aagaaggatt
     5821 aataattggt tcggcttgtg aagctggggt ggtttttcaa gctgtaaaga aaaacgtgtc
     5881 tgatgaggaa atgaaaaaaa taatagattt atacgattat attgaagtaa tgcctataga
     5941 taataataga tttatgattg ataaaggaga agtaaaggat gaagaagagt taagggagtt
     6001 gaataggaaa ttaattgata cagccaaaaa atttgacaaa attccagtag ctactgggga
     6061 tgttcatttt ttagacaaac atgaagctgt cttaagaaaa gttcttaaat attctcaagg
     6121 ttttaaagta gatgaagaag aaacttattt acattttaga acaacagatg aaatgttgga
     6181 agagtttagt tatcttggag aagaattggc atatgaagtt gtagttgaaa atagtaatct
     6241 gattgccgat atggttgaag atataaaacc tataccagat gatacttatc ctccaattat
     6301 agagggctca gatgtggagt taagagaaat gtgttacaaa aaagccaaac gaatctatgg
     6361 agacccaata ccagaaatag ttcaaaaaag actggataga gaattaaatt caataataag
     6421 caatgggtat gcagttatgt atattatagc tcaaaaactg gttgcaaaat ctctttcaga
     6481 tggatattta gttggttcaa gaggctcagt tggctcatct tttgcagcta ctatgagtga
     6541 tataacagaa gttaatcctc ttccagcaca ttatatttgt gaaaacgaag actgtaaata
     6601 ttcattcttt tatgaaatag gtgaatgggg ctcaggtgtt gatttaccag acaaggattg
     6661 tccaaagtgt ggaagaaaac ttgctaaaaa cggtcatgat attccatttg aggttttctt
     6721 aggttttgaa ggtgataagg aaccagatat agacttaaat ttctcaggaa attatcagcc
     6781 aacaattcat aaatacacag aagaactatt tggagagggg tatgtatata gggctgggac
     6841 tataggtact gtagcagaaa aaacagcttt tggatacgca agaaaatatg tggaagaaaa
     6901 taatataagt gtaccaaatg cagaagtact taggctttca aatggatgta ctggagtaaa
     6961 aagaacctca ggacaacatc ctggtggagt tatggttata ccagactata aagatgtata
     7021 cgattttaca cctatacagt atccagcaaa tgatacttct tgtggtgtta taactactca
     7081 ctttgattat cattcaataa gtggtagaat acttaaactt gatatacttg gacacgacgg
     7141 tcctaccatt ataaggatgc ttgaagatat gactggaata aatataacag aaattccttt
     7201 agatgataaa gagactatgt ctctatttac atctacagaa gcattaggtg ttacaccaga
     7261 agaaataaac tgtcctatag gttgccttgc tgtaccagag tttggaacta agtttgtacg
     7321 acagatgtta cttgacacca aacccactac atttgcagaa cttgtgcgta tatctggtct
     7381 ctctcatggg acagatgttt gggtaaataa tgcacaagac ttagttagag aagatatagt
     7441 tggattaaaa gatgttatat ctacacgaga tgacataatg aactatttaa tatttaaagg
     7501 attacctccc aaaatgagtt ttacaataat ggagagcgta agaaaaggta aaggtctaaa
     7561 accagaacac attgaagaaa tgaaaaaaaa taatgttcca gaatggtata taggctcatg
     7621 taaaaagatt aagtacatgt tcccaaaagc acatgctgtt gcttatgtaa tgacatcatt
     7681 tagaattgca tattgtaaag ttcactatcc agaagcattt tatgcgactt attttacgac
     7741 aaaagttgag gattttgatg cagatttaat tgttaaagga ttagatgcta taaaatcaaa
     7801 aataaatgaa attgagtcac tgggaaatga tgcaaccaca aaagaaaaag gtatgttgac
     7861 cgttttagaa gttgctcttg aaatgtatgc tagaggaata aaattattac cagttgatat
     7921 atataaatca gatgcaactg agtttatagt tgtaggagaa aaaacattac ttccaccaat
     7981 ggcagcaata caagggcttg gaggaaatgc ggctataaac atccaaaatg aaagaaaaaa
     8041 tggtgaattt atatcaaaag aagatttgag aaagagaaca aaaatatcaa aaacggtaat
     8101 agaaactctt actaatcatg gttcacttga gaatatgagt gagagaaatc aattgtcatt
     8161 gttttaagtt agcccaaaaa gttgcaaata aaagaatttt atgatatcat agtatagaaa
     8221 atagtatact ttgcagaaac taagagtgag acagctgttc tcactctttt attttaaata
     8281 tattattaaa aagttggttt atttttatta aatatatttt atttaagatt atataaacta
     8341 atcatttaca attgaatagg aggaacttta atgaaaaaaa atttagaagc tacaattgag
     8401 gagattgtaa ctaaaataac ggatgaacat ggatttgaaa tggttgatgt tgaatatgta
     8461 aaagaagcag gagaatacta cttaagagta tacatagata aagaagaagg aatatcttta
     8521 aatgaatgtg aattagttag tagagaatta agtccaatac tagatgaaaa agaccctata
     8581 aaagaaaatt actttttaga ggtatcttct ccaggacttg atagagcatt aaaaaaagac
     8641 agagattttg ttagatatca aggtagagat gtagatttaa aattatataa accattaaat
     8701 ggatgtaagc aatttgaagg tgaacttgta ggattaacag aagataataa tataaaaatc
     8761 attgcaaatg gtaaggaaat ggaatttaat agaaaagatg ttgctattgt cagattagca
     8821 ataaagtttt agtggtatat taaataggga ggatataaaa aatgaatcat gaatttatgg
     8881 aagcattaga tgaattagta gttgacagag gtatagataa agaaatactt atagatacta
     8941 tagaacaagc tcttttaact gcatacaaga aaaactttgg ttcagcacaa aatgttagag
     9001 ttgaatttga tagagacaag ggagatataa aagtattttc tcaaagagtt gttgttgatg
     9061 aatcagattt atatgatact ttcttagaaa tagagttagc ggatgctaga gagataaacc
     9121 ctaattatga attaggtgat attatagaac atgaagttac acctaaaaac tttggaagaa
     9181 ttgcagctca aacagcaaag caagtagttg ttcaaagaat aagggaagct gagagagaaa
     9241 tagtgtataa tgaatttata gaaaaagaaa atgaaatagt tacaggagag gttgctagag
     9301 caaataaaaa tgttgtttat gtaaatcttg gaagaataga aggtataatg actcaaagtg
     9361 aacaaatacc atttgagaat tataaagcag gtcaaaaaat aaaattctat atacttgaag
     9421 ttaaaaagac taacaaagga cctcaaatag ttgtatcaag aagtcatccg ggtctagtaa
     9481 agagattgtt tgaagaagaa gttcctgaaa tatatgaagg aatagttcaa ataaaatctg
     9541 tagcaagaga agcagggtca agaactaaaa tggcagtaaa atctatagat gaaaaaatag
     9601 acccaattgg agcttgtgtt ggaccaaaag gacttagagt aaaaaatata gtggatgaac
     9661 ttggcgatga gaaaatagat ataataaaat atagtgaaga cccagcagag tttatatcag
     9721 cagcacttag cccatcaaaa gttgttagag tagatataga tgaagatgaa aagtctgcct
     9781 tagtaatcgt tccagattat cagttatcgc ttgctatagg taaagaaggt caaaatgcaa
     9841 gactagcagc aaaattgact aactggaaaa tagatattaa gagcgaatct caagcagaac
     9901 aagaaatgtt aaatcttgaa aattccaaag aaatagaagc tgaggatgtt gaagctgaat
     9961 ttatcgacca agaagagtta ggtacttcag aggataattt tagtgaagaa gagtaaggag
    10021 ggatgatttt gaaaaaagta aaaaaagtgc ctcaaagaaa atgcatagca tgtcaagata
    10081 gagactctaa aaaagagctt ataagaatag ttaaaaataa agaaggaaaa atatttttag
    10141 acccaacagg aagagccaat ggaagaggag catatgtatg taaaagctct gaatgtctaa
    10201 aaaaagctat aaagagtaaa gcattaaata aagcttttaa aatagatgtt ccagatgaag
    10261 tctatgataa tctacttatg gagttagaag aatatgaaaa ataataaaga aaaagtactt
    10321 tcgttcttag ggctcgcaac tcgtgcaggg aaaatagtgt ctggtgatga ttcaactttg
    10381 cttgacctta aaaaaggaaa agtaaatttg attttaatcg cagaagatgc atcaaataat
    10441 actaaaaaac tttttaaaga taaatcaact tttagaaata ttccatatct atttttttca
    10501 acaaaagagg aaataggatt tgcaatagga aaatctccaa gagcagttgt aggaataaaa
    10561 gatgaaaatt tttctaaaaa gataattgaa ctaatagaaa tttaaaataa aatagggggt
    10621 gatcttgtgt caaaaacaag agtataccaa atagcagaag aattaaatat atcaaatgaa
    10681 gagcttataa ataagttagc tgaattagat ataaatgtaa ctgacaaaga ttcagtatta
    10741 gaaggtgaag agttagaatt agcacttgaa atgttaggtg aagatttatc tcaagaaaat
    10801 ggaaatgtta tagaaataga tggaaagttg acagtacaag tattagcaac gaaattagat
    10861 aaatctccat cagaaataat tatgaagctt atgaaaatgg gaactatggc aacaataaat
    10921 caagagataa gttttgagat tgctgctttg gctgccaaag actatggatt tgaattaaca
    10981 gtagctgaaa gtgatgatac agaagcttta gaaatagaag ctttaatgga aattgaagaa
    11041 gataaagaag aggatttaaa acctagacca ccagttgtta cagttatggg acatgttgac
    11101 catggtaaga catctttact agatgcaata agaaaaactg atgtgatttc aggtgaagct
    11161 ggaggaataa ctcagcatat aggtgcttca gaagttaaaa tcaatggaca caaaatagta
    11221 tttttagata ctccaggaca tgaggctttc acatcaatga gagcaagagg agcacaagtt
    11281 acagatatag caatattagt agttgcagca gatgatggta taatgcctca aacagttgaa
    11341 gctataaacc atgctaaagc agcaggtgta cctttaatag ttgctattaa taaaatagat
    11401 aaaccaggtg caaacccaga taaggttaaa caggaattag cagaccaagg attattagtt
    11461 gaagactggg gtggagaagt aatagcagtt ccagtatcag ctaagaaaaa agaaggaata
    11521 gatacacttt tagaaatggt attattggtt gctgaaatgg aagagttaag agctaatcca
    11581 aataaaagag cagtaggaac tgttatagaa gctgagcttg ataaaggaag agggccagtt
    11641 gcaacagtac ttgttcaagg tggtacttta acagtaggtg acccaatcgt tgcaggtgtt
    11701 gcatgtggta aagttagagc aatgataaat gcaaaaggta agagagtaaa aacagcagga
    11761 ccatctacag cagttgagat attaggtcta tcagaagttc cacaaggagg agaccaattt
    11821 gtcgaagttc caactgataa gattgctaga agtgtagcag caagaagaca acaaatagtt
    11881 agagatgaaa tgttaaaatc aactcaaaga ttatcactag atgctctatt tagtcaaatg
    11941 agtgaaggtt ctataaaaga ccttaatata gttataaaag cagatgtaca aggttcagta
    12001 caggcagtta aacaatcttt agaaaaatta tcaaatgaag aagttcaagt taaagtaata
    12061 catggtggtg ttggagctgt aacagaatca gacatattac ttgcagcagc ttctaacgca
    12121 ataataatag gattcaatgt aagaccagtt ccaggtgcag aatctctagg agaaaaagaa
    12181 aatgttgata taagaacata tactataata tacaaggcaa tagaagatat acaagcggct
    12241 atgactggta tgttagaccc tgaatatgtt gatgaagaaa caggaaaagc tgaaataaga
    12301 gaaatatata aaatatctgg tgttggaaca gtagctggat gttatgtgac taatggtaag
    12361 atatttagaa attgtaaagt aagattagtt agagatagca taataataca tgaaggtgag
    12421 cttgcagcac ttaagagatt taaagatgat gtaaaagaag ttaattctgg atacgaatgt
    12481 ggtatgagtt ttgtaaatta taacgacata aaagaaggcg atatagtaga agcttatata
    12541 actaaagaag tagaaagaaa attataaatt cgtatcaaag aaaatcagtt agtaaagaag
    12601 gtcgatggta tggcatcata taatagaaca agaaggatag cagaagaaat aagaaaagtc
    12661 gtaagtacta tgcttataaa tggagtaaaa gaccctagaa taacatctat ggtgagtgta
    12721 actgatgtag aagttacaaa tgacctaaga tatgcttatg tatatgtaag tattctaggt
    12781 ggagatgaag aatccacact aacaggtctt aaaagtgctg gaggatatat aagaagagaa
    12841 gtaggaaaaa atataaaact tagatatatt cctgagatag tatttaaact tgatgattca
    12901 atagaaaaag gaatgtatat ggataacctt ataaaaagag ttaatgaaaa aaatgctcaa
    12961 caaaatgagg atgaaaatta tgatgaataa tatggatagt attataaact atatttatga
    13021 gggtgactat tttatagtta cctctcatat cagtccagat ggagacaatg ttggttctac
    13081 actttcaatg tattatacat tgaagaattt aggaaaaaat gtatattatg tacttgatga
    13141 tgaagctcca ttaaatttaa ggtttttggt tgaaggtgta aaaatctata agtctaatga
    13201 atttaatatg aagaactatt ctttaattgc acttgattgt ggggataaga tgagaatatg
    13261 tgtgagtgat gaaatcaaaa ataatgcatc aaaattaatc tgtattgacc atcatgctag
    13321 taatgattca tatggagatt taaattatgt agatattgat gcatcttcta catgtgaact
    13381 agtttataat ttattggtta gatttcagca aacaaaagat attaatatta taaatgaaga
    13441 tatagcaaca tgtttatata caggtcttgt aactgatact ggaaactttt cgtattcaaa
    13501 tgtacatgca agtagttttg aaatggcaaa aaatttactt gttttaggtg cacaaaaaaa
    13561 tactataata caaaatatat atcaaagcaa ttcttctgat tattataaat tattaggaga
    13621 agctctaaaa ggtttggaga tatttgattc aaaggtatct agtatagttt taactcaaga
    13681 catgatgaat agaaataata ttagctttaa tgatgttgat ggaataacaa catatacaag
    13741 agatataaaa ggtatagaag ttggaatttt atttaaagag aaaaaacaaa atgaaattaa
    13801 agtgagtttt aggtcaaaaa attatgttga tgtaagtgaa atagccaagt tatttggcgg
    13861 tggtggtcat gtaagggctg ctggatgtac tattagagat agtattgata atgcaaagaa
    13921 aatggtacta gaggcagtgt taaaatcaat ttaaggtgac agtaaaatga ataagattat
    13981 aagtatattg aaacccacag gtatgacatc tcatgatgtt gtaagtagag tgagaaagat
    14041 tttaaatata aaaaaagttg gtcatacagg tactcttgac ccagatgctt caggtgtttt
    14101 accaatctgt atagggaaag ctacaaaagt atgtgaagtt attttaaaca aagataaatc
    14161 gtatatatgt gagcttactt taggtataag tacagatact tatgatgctt caggtgaaat
    14221 tttaaaaaaa gttgatgatt ttaaatttag taatgaagac atagaaagag cttttgatac
    14281 acaaagagga gagataaatc aacttcctcc aatttattca gctttaaagg taaatggaaa
    14341 gagaatgtgt gatttagtaa gaagtggtag acaatctgaa attactttaa aaacaagaag
    14401 agtaaatata aaagacataa aaatactaag tataaaaggt aataaagtaa tgttttatgt
    14461 tgagtgttct aaagggactt atgtgagaag tatttgccat gatataggag aatatctggg
    14521 ttgtggagct catatgtcgt ttttgaatag aacatcttct ggtaaatttg acttagataa
    14581 ttcaataaca ttagaagaat tagaattatt ttatgaaaat aaaactttag acaaatactt
    14641 atatgatata gattatgtcc ttgattcgtt taattatgtt gtattaaacc ctaatgctat
    14701 aaaatattat agtaatggtg gctcaataga tgataaaagg tttttaaaaa ataatttcga
    14761 taaagatgat gaagttgtaa gagtatacag tacagataat ttcttaggat taggaaagtt
    14821 atcaaaacac aataatacaa taagtgttaa aagtgataag atgttcatat aaccaatgta
    14881 tttaaaaaaa caacaaaaga gatagtacta taataacttc tatagtctat ctcttttact
    14941 tatgctataa tagtttgtga ataagttaag agtaaattat attttagcgg ggaacattta
    15001 ttatggtgat tataaaatct ataaaagaaa tagcaaacat aaaagaaagt gtcattacca
    15061 taggaaattt tgatggtgta cacaaaggac atcaagttct tataggaaaa acagttgaat
    15121 atgcaaaaaa agaaaatata cagagtattg tatttacatt tgcaaatcat cctgtaaatt
    15181 actttagacc aaactcaaca aaaaatatta taagcaatga tgataaaata aaattattag
    15241 aagatttggg aatagatata gttgtaatca taccatttga tgagtatatg actaaaatac
    15301 ctgcaaagga tttcgtagat gagatactag taaaaaaatt aaaagctaaa aagatagtaa
    15361 ttggacatga ctttacattt gcaagggcaa aagaaggcaa tgttaatcta ttaaaaagct
    15421 tagaacatga atttggtttt gaggtagaag taataaaacc catcaaaatc aatgatgtaa
    15481 gagtgagtag tacttatatt agaagtttag tatcccaggg tgatatggct aatgtcaagg
    15541 aatatttggg tagaaattac aagttagaag gttgtgttat acattctaaa caccttggaa
    15601 gaactatagg attcccaacg gcaaatattg accttaagaa taatatgctc gttccaaaga
    15661 gaggtattta tgcttctatt gtacatattg gagatgagac ttattttggt gctacaaatg
    15721 taggctataa cccaacagtt aatggaaaaa ctttgtctat tgaaacaaat attttagagt
    15781 ttgatagaga tatttatggg gaaaatataa ttgttgaatt tttggaaaga ataagagatg
    15841 aaagaaagtt taattcaata gatgacttaa aaaatcaatt gtataatgat acaaattatg
    15901 tgtatgaaaa ctatgtttgc aaaaacaagt aatgtatgat aaaatgatta aggaaaataa
    15961 ccattactcg gcatttgaat actccaatca atgcttagta ataggcgttt ctaaaaaaat
    16021 acggaggtaa tctaaatgat aaacaaatca gaaataataa aagaatatgg aagagctgaa
    16081 ggggatactg gttctccaga agtacaaata gctttattaa cagctagaat aaacgagtta
    16141 aatggacatt taaaaactca taagaaagac caccattcaa gaagaggact attaaaaatg
    16201 gttggtagaa gaagaaactt attagcttac ttaaaggaaa aagatcttga aggatataga
    16261 gcattaatag ctaaattagg attaagaaag taatttcatt ctttataaaa aagcaggtat
    16321 agtgtatacc tgctttttta tagttaattc attataaagt aaaatagtta ataataaaag
    16381 tagtttatat gttataaaaa attataaaat gaataatatt gggtaagaat attaaaatag
    16441 tatacaaata cataatcaat ttaaaaggaa agacaggata aatatagaaa ttttataaca
    16501 ggataattag ttggaataag gaggatatta ttttgaaaat aaagttagta tgcgatagtt
    16561 tgtgcgatat tccagatgaa atatcagaaa aagattatat agagatagtt ccacttacag
    16621 ttatttttaa tgatagggaa tatatagaag gagtagatat aaagaaagaa gagttttata
    16681 aaaaagtaaa agaaatcaag caaataccta aaacttcaca agctacatat atggagttta
    16741 aagaagtgtt tgataagttc ataacagaag gttatcatat aatctgtatg acaggggctg
    16801 caaacgcatc aggaactttt caaagtgcta tgatagctaa aaatgatgta aatgaaaatg
    16861 aaaaaataca tatatttgat actagaaatt tatctctagg tagtggacaa tatgttataa
    16921 aggcatgtga acttcttgaa gaaggactgg gatttgaaga aattatagat gaattagaaa
    16981 atactcgcag tagtgtaaga ttactttttg caccatatac acttgatttt ttaaagcaga
    17041 gtggaagagt tccagttgca actgctttga taggaaatat gttaaatata aagcctatat
    17101 ttttctttga taatggggaa gctaagttgg taaataaggt tagaggaata aagaatatag
    17161 caagtaaact ggtcgatata attttagaaa tgaatgaagg gtctttagaa ggaaaaatcg
    17221 tcacaatagg ttgtggagat aatttacacg attgtgaaat attaaaagat gaagtaaata
    17281 aaagaataaa agcaagaaga gtattattta ctcgaggagg agtttctatt tgctctcata
    17341 cagggccaga tatattggct ataagttgtt ctaattagta gtaatataag gtttttgcat
    17401 atatatgtga agataatttt atattgttaa tttatagtat ttttgataat atagtaaata
    17461 gagtgatata cgtgtttttt aataagagga ggacaagcat taatgtttga acataaaata
    17521 tttaaaatgg attttgcagg aagagaactt tctgtagaaa taggaaagat ttgtgaaatg
    17581 gctagtggta gttgtatagt tcgatacagt gacagtatgg taatggtaaa tacaactaaa
    17641 tcagccaaac ctagagacgg aatagacttt tttcctttaa gtgtggatta tgaagaaaaa
    17701 ttatactctg taggtaagat acctggaggt tttttaaaga gagaagggaa gccttcagaa
    17761 aaagctatac ttacatcaag attgatagat agaccaataa gacctttatt cccaaaagga
    17821 tttagaaatg atgttcaggt agttgcaaca gttctatcag tagaccaaga ttgtacacca
    17881 gatatagtag caatgatagg ttcttcaata gccctttcta tatcagacat accatttaat
    17941 ggaccaacag gttcagtatg tgttggcctt gtagatggtg catttgttgt aaatccaaac
    18001 gcagagcaaa gagaaaaaag ttcaatgcat ctagttgttt caggtacaaa ggaagcaata
    18061 atgatggttg aagccggagc tgatgaagtt ccagatgagg taatgcttga tgctatttta
    18121 tttgctcacc aagaaataaa aaaaatagtt gagtttatag aaggtatagt tgcagaagtt
    18181 ggaaaagaaa aaatgcctgt tgaattatac catgcaggag aagaaataac tcaattggtt
    18241 cgcgaatttg caactgataa gatgaaaaaa gcagttcaga cttttgaaaa gttagagaga
    18301 atggaaaata tggatagagt taaagaagaa actttagctc actttgaaga aactttagaa
    18361 gattttgaag attttgttgg agatatagaa gaagtattac aagatataat aaaagaagaa
    18421 gtaagaaaac ttatagtaca tgaaaatgta agaccagata atagaaaact tgaagaaata
    18481 agacctatat ggtgtgaaac tggcatgata cctagagctc atggttcagc catatttact
    18541 agaggacaaa ctcaagtact taatgttgct actttaggtg ctttaggaga tgttcaaaaa
    18601 ttagatgggt tagatgaaga agaaaacaag agatatatgc atcattataa tttccccgca
    18661 tatagtgttg gagaagctag accatcaaga ggaccaggaa gaagagaaat aggacatggt
    18721 gctttagcag aaagagcttt attaccagta ataccatctc aagaagaatt tccttatgct
    18781 attagattag tttctgaagt attaagttca aatggttcta catctcaagc aagtgtatgt
    18841 ggctcaacat tatctttact tgatgcaggt gtacctataa aagatatggt tgcaggtata
    18901 gcaatgggac ttataaagca tgatggtaag gttgcagtgc tttcagatat acaaggtatg
    18961 gaagaccatt taggcgatat ggactttaaa gtagctggaa cagagtatgg tataacagca
    19021 atacaaatgg atataaaaat tgatggtata gataaagaaa ttctacaacg tgctttaaaa
    19081 caagctaaag agggtagaat ccatatacta ggagagatga gaaaaactat atctcagcca
    19141 aaaccagaac tttcaccata tgcacctaag attgttaaaa tgcaaataaa tcctgacaag
    19201 ataaaagatg taatagggcc tggtggaaag ataataacta agataataga cgaaactgga
    19261 gttaagatag atattgaaca aactggggaa gtatttatat caggtattga gattgatatg
    19321 attaagaaag ctcaagaact tataaataat atagtagttg aaccagaagt aggaaaaact
    19381 tataaaggta aggtaagtag aataatgaac tttggagcat ttgttgaaat acttccagga
    19441 aaagaaggat tacttcatat atctcatata gctcatgaaa gagttgccaa agttgaagat
    19501 gtattgaata taggggatga agttgaagtt aaagttacag agattgatga aaaaggaaga
    19561 gttaatttat caagaaaagt tcttttacca aaaccagaac ataaaaataa ataacttatt
    19621 atataattaa aagcaaagtc ttaggatttt gcttttttaa atacaaaaaa gtagataatt
    19681 ttaaaatgat agttatggat ggcttatagt tctttttctg taaggcttta ctcttataaa
    19741 gaagctattt aaaagctgat taatagaaag ctttattgat atatatagaa tcacttaagt
    19801 tatatttagt gcataaacag agcttatttt taatagtatt aaataagaaa caatttgtac
    19861 ataattaggg ggagttttaa tgataatgat ggtacttaat aagagtaagc tcaaaaaaat
    19921 cataatacta attttagtag tgttattagt attatcagga ggggtaatgg tattaaagtt
    19981 atttaacaac aagccagtat ttaatttatt taatggtgta gatttaccat atgaaagagg
    20041 tacaaaggat aaaagtggat atgttgcatt aacctgtaat atagaccttg gatgggaaac
    20101 agagtacgta gaatccattt tagaaacact taaaaaagaa aatgtaaaga ttacatttaa
    20161 tgtaactgga aaatgggcag aaaagaataa ggatgaatta ttaaagataa aaaaacaagg
    20221 acatgaaata ggtaatcatg gatataaaca tttagattat tctacattat catatgaaga
    20281 caattatgag caaatagaga cctctaaaaa gataattgaa gaaattatag gtgaaaaaac
    20341 taaatttttt caagcaccag caggttcctt tggtccagaa acagtaaagg ctgctaaagc
    20401 attagggtat acttctatca aatgggatgc cgatactata gattggaaat acaaagacca
    20461 accagaggta atcattgata gaatgaagaa aaaagacatt aaagatagta gtataatctt
    20521 aatgcatcca accaacgcaa cgacaaaatg tatagatgat attatagcta ttgtaagaga
    20581 aaaaggacta aaaccaggga aacttagtga tgtatttaag tagacaaagt ataaatattt
    20641 ggttaagatt gtataagttg tagttgaatt aattaaaaga ctgacttata atgtaataca
    20701 tataaaaaat aaatataatt aaaacatagt gttttcaata atccaatatg aaacaaagaa
    20761 taaattgtta ggagaatgta atgtataaga caaaaatact ggaaaatggg cttacaataa
    20821 ttggtgaaga aataccatat ctaaagtcaa taacattagg tatttggata aatgcaggct
    20881 ctaggataga agaagcacag gtgagtggaa cttctcattt tatagagcat atgatgttta
    20941 aaggaaccaa aaacagaaca tcaaaagaga ttgctagttc aatagataat ttgggtggtc
    21001 aaataaatgc atttacaagt aaagaatgta catgttatta tgtaaaacta atagatgagc
    21061 atatagatac gggaattgat gtattgagtg atatgatact aaattctaaa tttgataaaa
    21121 atgatataga taaggaaaga cttataatat tggaagaact taagatgtat gaagactctc
    21181 cagatgactt atcttatgat ttattagtgg aaaatatata tgctaatgat ggattaggaa
    21241 tgaatataat tgggacaaaa gaatctctat acaatataac tagagaatcc atgttagaat
    21301 acttaaataa gtattatatt ccaaataacg ctgttatttc tatagctgga aactttaact
    21361 ttgatgatat ggtggagaaa atcaaatcaa aatttggaca ctgggaaaag aaaaatttga
    21421 gtatagacat aagtgaggct aagtttaatc cttgctttat atcaaaaaat aaagatactg
    21481 aacaggtgaa cttagctatc tgcctaaaag gtattccttt tgaaaatgat gaagaagtct
    21541 actcaatggc agtggtaaat aatattttcg gtggaagtat aagctcaagg ctttttcaga
    21601 aaataaggga agaaaaaggt ttagtatatt caatatactc atcacagaca ttatatagga
    21661 aatgtggtga attaggcata tttgcaagta tgagtacaga aaatttacaa gatgtatata
    21721 atttaataaa gaaagaaatt gaaaatataa gagaaaacta tcttacagaa aaagaaatat
    21781 ctgaaagcaa ggaacagtta aaaggaaatt atatattgga tttagaaagt actagcagta
    21841 gaatgatgtc tactggtaaa tctatgttac ttagcaaaaa agtaaaaact acagatgaaa
    21901 tacttgaatg tataaataat gttaatatta attcaataaa gaaggttgtt gataaggtat
    21961 tcaatatcga aaatattggt acatgtatag ttggaagaga tgtagaaaag attctacact
    22021 tggattaata ttgaaaaaag taaatctctg gaggtataaa atatgaattt atctgaaata
    22081 gcaggtaagg aaatagttaa cttggtcaca ggagaaagat tgggggtcat tggagaatgt
    22141 gaccttatac tagatgaaac tacaggcggt atattagcgc ttctaattcc aaaggaaaaa
    22201 ggattttttg ccagaagaaa agataagtca tttttagaag taccatggag aaatgtaaaa
    22261 aaaataggta atgacatgat aattatagaa tatgagggat atatttagga ggaaaaaatg
    22321 agtattttag tgcaaaagtt tggtgggaca tcagtagaat catatgaaaa aatgaatgaa
    22381 gtatgtaaga tagtaaaagc atacaaaaaa aatgatgaag aattacaatt agtattagta
    22441 gtttcagcaa tgggaagaaa aggagctcca tatgctacag atacacttat aaatttgtgt
    22501 agtgcagtaa atgatgagcc aagtaaaaga gaacttgatt taataatgtc ttgtggagaa
    22561 ataatatcag gaacaattct tgctaattta ttgaatgcac aggggataga ttctgttttc
    22621 tttactggtc agcaagcagg tataattaca agtgatgaat attctaatgc aaagataaag
    22681 tatataaatc ctaaaaaggt aaaaagagct ttagatgatg gtaaggttgt agttatagct
    22741 ggattccagg gtgtaacaga tgatggagaa ataactactc ttggaagagg aggaagcgat
    22801 acatcagcag ttgctattgg taaagcttta gaatgtgaaa cagttgaaat ttacactgat
    22861 gtggatggta taatgactgc agaccctaga gtagaaccaa acgctaaagt attgagtttt
    22921 atagattatg aggaagtatt ccaaatggct gacaaaggtg caaaagtaat acatccaaga
    22981 gcagtacagt tggctaaaag tggaaatata actttagcca taaagaatac aatgaatcca
    23041 acttttgaag gtacaaaaat aggttcttta tgtagacact tagaagagaa tatagaatat
    23101 gaacaagaaa gagattttaa ggttgctgtt gcaaataaag atagtgtagc acaagttaag
    23161 attaaatcag cagaagaagt gtttacagaa gtattaaatg agatagaaaa gaaacttata
    23221 actatagata tgataaattt ctttatatca gaaaaagcat ttgttgtaga agatgctgat
    23281 ataaaatcat taaaggaaat actagataaa tttgaattag attatgaagt aaatagagac
    23341 tgtgctaagg taacattgat atgttcaaga attgctgatg aaatgtctgg tatcatgtca
    23401 aaggtagtta gaggattatc aaaagcaggg gtatctcttt tacaaacatc agactcaaat
    23461 atgactatat cttgtttagt tagtgaagaa gatatgcata cagcagtaca tgctatacac
    23521 caacaatttt atttaaaata aactgtataa tacaattatt ttttaatcaa atttaagaat
    23581 ttcatatata tcttaatata tttgatttat ttggtaaaac taaattaaat ataattttga
    23641 taggagtttt tttatgaaag attatagaga agataatgaa agatttgata aagataattg
    23701 tagtgaagaa tctagttgcg aagagagaga atgtataaag caatttggta ccaatgaaat
    23761 gccacctcaa cctcctaaag atattcaatg tataactata attggagaaa ttgagggaca
    23821 ttttatagga aatcctcaaa aaaaagctac aaagtacgag catataattc caatgctata
    23881 ctcaattgag gaaagcaatg atgttaaagg agtgttagtt gttttaaaca ctgttggagg
    23941 agatattgaa gcaggtttag ctattgcaga acttttaaac agtacatcta aaaaagtagt
    24001 tactcttgtt ctaggaggaa gtcatagtat aggtgttcct cttgctacag caggagatta
    24061 ctcatttata gcaccaacag caaccatgat tatacatcca gtgcgaacta ctggtcttgt
    24121 gattggtata aatgagactt ttgaatattt taaaaagatg caagatagaa taattcaatt
    24181 tataataaga acatcaaata tcaaaaaaga tgtacttgaa aaacttatgc atgaaaaaga
    24241 tgaacttgta agtgatgttg gtagtgtatt aataggaaaa gaagctgtgg attatggtct
    24301 tatagatgaa gttggaggac taaaagaagc acttaaaaaa ttaagagagc ttataaaaga
    24361 atctgaggaa gaaaaaaata atgattaaaa tttgtttaaa taataaaatt tatgatataa
    24421 tgatgtaata aagtaggagt gttagtgtta aggcattagc actttttgct gtgaaggggt
    24481 aaaacttgct aaaaataagg agtgatatta atggccacga aaaagaagaa aaaaaagaag
    24541 gtaaatacct taagttttaa tgcagaatat cataatttaa taacaatatt tataggtgta
    24601 tttttattat atagtttaaa ttctaattct atgggatgga tacctgtgtt aatgcaaaat
    24661 ctttttaagg gactatttgg agggctttca atagcaatac catttatagt aattataaca
    24721 ggacttttag gattttttga tggaaatgaa tatatatata ggctaagaaa gactaaacta
    24781 tactacataa ttattttatt tatatttgta ttttatggat tattaaatgc aggaactctt
    24841 cctgtagata gcccactaaa aggtaacatg tttgatgatg ttatgaaatt gggtgtaagt
    24901 ggacaaggta gtggtttgat agcaacaact attgcgtatt atatgagtaa aatatttggt
    24961 atagcaggtg gatggttaat aagtatcttc gcacttatac tttctgtaat gtttatattt
    25021 aatatttcta taaaagattt ggtgtcaaat gcaaaatcaa aatctaaagc atctaaagac
    25081 agcaatttaa cgtttaaaga taaaattgca aatatgaaaa aatctgcaat agatatgatg
    25141 actgatgaag tagatgatac tactataaat aaaaaaccag gtttctttaa agggttaatg
    25201 tcaaaaggtc ttaataatga tgatgaagaa gatgaatatt ttgaagaaga aaatagtgat
    25261 ggtgtagatg ataaaactat aaaaatagtt ggatttaata aggcagaaga tgagtattta
    25321 gaaatattag aaggaactca aagtatgcca gagttagatg ttttaaaaga attacaaaaa
    25381 gctaccaatg aaaatccagt tgtagatact aaaccagaaa aaaaagttga tatagctaaa
    25441 tctaatttaa atatagaaaa aactcaacct atgagtattg tagcagagcc agttaatgag
    25501 gattattcaa actataaaaa gcctagcatt gagcttttaa ataaagtaaa taagaagtct
    25561 gatgaaaatg gtaaaaagaa agtgttgaaa aatgcttctc ttttagaaaa gacactatca
    25621 gattttggag tggaagcaaa aattaatcaa gttacagttg gacctactat aacaagatat
    25681 gaaatacaac caagtccagg agtcaaggta agtaaaattg taaatttaac tgatgatata
    25741 gctttaagtt tagcagctaa aagtataaga attgaagccc caattcctgg taaaagtgca
    25801 attggaatag aagttccaaa tgaagaagct caaatggttg gtgtgagaga agttctagaa
    25861 agtgaagaat ttaataattt taactctcct cttgcaatgg gacttggaaa agacgtagca
    25921 ggaaaaatta taattggaga tataggtaag atgccacact tactaatagc tggttctaca
    25981 ggttcaggaa agagtgtgtg tgtaaataca ttaataagta gtattttata taaggcaaat
    26041 cctgatgaag taaaattact attaatagac ccaaaagtag ttgaacttgc aaattataat
    26101 ggaataccac atctattgat accagtagtt actgacccta aaaaagctgc aaatgcttta
    26161 aattgggcag ttacagagat gaatagaagg tataaattat ttgcagatgc tcaagtaaaa
    26221 gatgtaacaa gttataatga gaaagcggaa gaaaaacttc ctaaaatagt aataataata
    26281 gatgagttag cagatttaat gatggcaagt gcaaatgatg tagaagatta tatttgtaga
    26341 ttagctcaaa tggcaagagc tgctggaatg cacttgattg tagcaactca aagaccatct
    26401 gttgacgtta taacaggggt aataaaggct aatataccat ctagaatagc atttgcagta
    26461 tcatctcaaa ctgattcaag aacaatcctt gatatgggtg gtgcagagaa gcttctaggt
    26521 aaaggtgata tgttattcta tccacttggt gcagcaaaac cagttagact tcaaggggca
    26581 ttcatatcag agagtgagtc ggaaaaagta attgattttg taaaaagtca ggttaaagat
    26641 ggaataaaat atgaagaaga tataattgaa actatatcta aggttaatac ttcaaagagt
    26701 agtgatgagg atgagttttt gagtgaagcg atagaatttg tcgtggaaag tggtcaagca
    26761 tctgcctcta tgctacagag aagatttaaa ataggattca atagagcagc tagacttata
    26821 gattctatgg aagaaagagg cataattgga gcaagtgagg gaagtaaacc tagaaaggta
    26881 ttaataagca aacaggattt gcagaattta gaaggtgaat agaattgtca gttataaaaa
    26941 tagaaaaagc acttaagcta gataaagtaa tatttgtaga tgtgagaact gaaggagaat
    27001 atgaagaaga ccatatattg aatgcaatca atatgcctct ttttaaaaat aatgaacata
    27061 atgaagttgg aacaatttat aagatgcagg gaaaacatga agcaatacaa aaagggtttg
    27121 actatgtatc gtataaatta aaggatatat atttacaagc agcagaactt gcattaaatt
    27181 atgataatat tgtcatatat tgtgcaagag gcggaatgag aagtggaagt atagtaaatt
    27241 tattatcatc attaggtgta aatgtatatc aacttgaagg tggatataag gcttatagaa
    27301 attttgtatt agagtatcta aggcatgtaa tggatacaaa aaactttgta tctgttcatg
    27361 gattgactgg tacaggaaag actgatttgt tacatttatt agatgaaaaa ggaatagatg
    27421 tactggattt agaaggcata gctaaaaaca gtggttctac ctttggtttt ataacttttg
    27481 ataaaaagcc accatctcaa aagaactttg aaacaaaact atttgaaagt gtattttttt
    27541 cgaaagagaa ttatatattt gtcgagagtg aaagcaaacg agttggaagt gttacagttc
    27601 caaatgaaat tttcgaggca atgacacgag aaggacatca tatattatta gaatgtgata
    27661 ttgaaaatag agttgataga ctttgtagag attatattta tgataaagat gaaggtaata
    27721 tattgatttt aaaagaatgt ataaacaagt ttagaaaaag attgggtaat aaagtagtag
    27781 atgattatat tgatttatta gaatcaggtg aatataaaga acttgtaaaa agatatcttt
    27841 tagaatacta cgacccactt tatatgcatt cagtagagaa atataagtat agtaagatta
    27901 taaattttaa tgatacacaa aaagctttag aagatgttat agacttatat gagagtatat
    27961 tggaaggaga aagtgaatgt taaaaatagc attggaatca ttaggatgct caaaaaattt
    28021 agtagatgca gaaataatga tgggaatttt aaataacaaa ggctataaat taattggaga
    28081 ttttgaagaa gctgatgtaa ttatagtaaa tacatgtggg tttatagagt cagctaaaca
    28141 agaatctata gatacgataa taaacttttc agagttaaaa aaaactggca atttaaagtt
    28201 gttgatagta acagggtgtc tagcccaaag atattctgaa gaattaaaaa ctgaaattcc
    28261 tgaaatagat gctatagtag gaacaggaag ctatcaaaat atagataaaa tacttaaaga
    28321 attaagtgaa atgcatcaaa ttgtaagcct taatgatata gagtttgtat ttaatgaaga
    28381 tttaccaaga tatatatcta cacctagcta tatggcctat ttaaaaattg gagaaggatg
    28441 ctccaataat tgtacttatt gtataatacc aaaactaaga ggtaaatata gaagtagaaa
    28501 atttgaagat ataattaaag aagctaagaa attagctgaa tctggagtaa aagaacttgt
    28561 ggttatagca caagatacga caaagtatgg ttttgattta tatggaaaag aaagattatc
    28621 agaactttta gaagaacttg caaaaataga tggatttaag tggataaggg ttatgtattc
    28681 atatccagaa tctataacag aggaattgat acaagttata aagaaatatg ataatatatg
    28741 tagttacttt gatatgccaa tacagcatgc aagtaacaat attttaaaat taatgaatag
    28801 aaaaactact aaagaagata tattaaataa aatcaatctg ataagaagta atataccaga
    28861 tgctatactt agaactacga taatagtagg gtttcctggt gaaacagaag atgacttcaa
    28921 acaacttgtg gactttgttg aagaggttaa atttgataga cttggtgcat ttgcatattc
    28981 aagagaagaa gatacaccag cagatagatt gccaaaccat atagatgaag aagtaaaaat
    29041 acaaagaaga gatactttaa tgatgattca acaaaaaata tctgaagaac ttaatgacaa
    29101 aaagatagga aaaacttatg aggtgttaat agaagaacag atagaagata atgtatatac
    29161 aggaagaaca caaggtgatg cggaagaaat tgacagtata gtgtatgtaa aaagtgtaga
    29221 caatcttgaa gtaggagaat ttgtaagtgt tcaaataaat gatgcaatgg aatacgattt
    29281 aatgggagat gttttatatg aacttgccta acaagttaac tttgtttaga atatttttaa
    29341 taccagtatt tgttttaatt atgttgctca atgtaccaaa taaattttta atagcttgta
    29401 ttatttttat aatagcttca attactgatg ctttagatgg aaaaatagct agaaaataca
    29461 atctagtcac tgactttgga aaatttatgg acccattagc agataagtta ttggttattt
    29521 cagcacttac atgtatgata gaagaccatc ttgtaagtag ttggatggta attataatag
    29581 tggctagaga gcttacagtt agcatattaa gagctatagc tgctgctgat ggaaaagtaa
    29641 ttgctgctgg aaacagtgga aaacttaaaa cgataactca aatggtatct atagtgtttt
    29701 tattgttagg cgctcagttt gaaaatgtat tgatattaaa tattggtgaa attctcattc
    29761 ttatagctac actacttaca ttatattctg gatgggaata tctatataaa aataaagagc
    29821 tttttatgtc ttctaagtaa aaaattatgc tttaataaat aaaaactaat gattaataaa
    29881 taaaaaataa caacttaaaa ttttaaaaat gta
//
LOCUS       DAMJVX010000028        29102 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMJVX010000028 DAMJVX010000000
VERSION     DAMJVX010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29102
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.030"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            235..2877
                     /gene="adhE"
                     /locus_tag="PC407_001229"
                     /gene_synonym="adhC"
     CDS             235..2877
                     /gene="adhE"
                     /locus_tag="PC407_001229"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2496686.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPDRVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGNTDQEKVDNLIKAIDELKARVGLPKTIKEAGVEESKFLETLDVMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(3237..3578)
                     /locus_tag="PC407_001230"
     CDS             complement(3237..3578)
                     /locus_tag="PC407_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496687.1"
                     /translation="MLLILDNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRINKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(3605..4276)
                     /locus_tag="PC407_001231"
     CDS             complement(3605..4276)
                     /locus_tag="PC407_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2496688.1"
                     /translation="MPILEILNLGKIYGKKETAVHALKDANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSSGTIKVDGKDICCLSDKKLAKYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKVDEEYIEDIINLLGLSERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(4288..6873)
                     /locus_tag="PC407_001232"
     CDS             complement(4288..6873)
                     /locus_tag="PC407_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2496689.1"
                     /translation="MKDNLKIALRYIISYKARSLAIALSIILATSLIVGIGTLSRSAQ
                     QAEVDKLKRETGSDHVYFKDINKNQLEYIKSRKDIKNLGITSHYGYTDPNERLAINLE
                     YANKNYLTNQSKLIKGHLPKASNEVVVEKWILNSLGLKPEINQNITFKLYQKEKPETF
                     KVVGILEDRPIEKNKGTCEIFLNLNESKLDKFSYAYIEFNNGIDINTSIDNIVKNTML
                     DENSVGKNKMLIESTRENGTLDNSSKYTAITMSLFSGIVIYSIYVISIYQRIQEYGIL
                     RAIGSTNFKIFKFMFYELFILALIAIPIGICTGIGGAQIFNRTAGNIQFEGNVTVTPF
                     VIPDKIILLSIGSIILTILIISFFTYLKIRRISPIDSIRKTFGTDKNINKVNSLISKL
                     TLNISVTKSISAKNIFRNKKGFIIIILSMSLGGIMIIKENYKYSFSDIQNKNGQEETY
                     MNADFILTNFFLKLNQSNKADSFKDIKGLNDNQIDKIKNINGIDKVKTASILDTRIEV
                     DKLNGLDYYNIINSTPYYKDFPLFIKNKNTGKYTMKQKLRGYNDEMINSLEKYLVSGS
                     INLERMKKENLAILYVPQVSKTNKYKLSYTPGTETPAVDIKVGDTIKVKYPKGEIDQD
                     LYIKLKDNYEYLEYEFKVGAIVSYPFADNYLYSGDQGIDVITSNDYLKKLTGVDKYNV
                     VYVDINKNANVKKINTLLGEIGSESPGTTTANMLMEKENFDKMTARALTYAYGIVAVM
                     FIISVFNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGILSSVITIVV
                     GLIIQFRMYYTYNFVSYGLGFSIDYKIYILVVLANIIVGILATYIPLRKINKISIVEA
                     INITE"
     gene            7081..8016
                     /locus_tag="PC407_001233"
     CDS             7081..8016
                     /locus_tag="PC407_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2496690.1"
                     /translation="MENNFIFPIVILTLFIFSNLIMNKYRFYKELDILKRGILTFFVS
                     CFVWILFMEGIEYIQLSLGYNFLLEGVLNIILFLILIILSFYIGFKFIRLLDNNNKQE
                     YKLQKLDSKINHKDNIIVKLRSQKHDYLKHLQVVYSLLNTGLAEDAKEYITNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKNISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSAIQKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFLDGFSTKEGQDRGF
                     GLYLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            8035..8772
                     /locus_tag="PC407_001234"
     CDS             8035..8772
                     /locus_tag="PC407_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2496691.1"
                     /translation="MIKVLICDDDKIVRDDIANIVKESGYVDYVKKVKDGIEAIEFIK
                     SEKIDLLIIDIDMPYKNGIDSAKEILSIDKDIHIVFVTGFADYSLESFKVHPIDFIVK
                     PFKKEKILDSINIAIDHINSHKIAKNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLYTRDEDTIKFYENFDDLKKRLPPNFFMTHRQYIVNLRLIHKVIPINKSSLEIKF
                     INFQESATLNKSLEKDFLYRFYRVKRF"
     gene            complement(8932..13989)
                     /locus_tag="PC407_001235"
     CDS             complement(8932..13989)
                     /locus_tag="PC407_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDF2496692.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVTSALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNSDRQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTSEDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKFKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGIDYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDESGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQLVQPFKQVFREYYRINSDEISEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNINFEGSHV
                     LITGNLGEYSVHMGSGIVHKKGMGALNILPVHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDNKIKDPSILSNL"
     gene            complement(14014..14433)
                     /locus_tag="PC407_001236"
     CDS             complement(14014..14433)
                     /locus_tag="PC407_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496693.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSPINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(14666..14917)
                     /locus_tag="PC407_001237"
     CDS             complement(14666..14917)
                     /locus_tag="PC407_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496694.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGTKFINKKR"
     gene            15006..15737
                     /locus_tag="PC407_001238"
     CDS             15006..15737
                     /locus_tag="PC407_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDF2496695.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            15796..16707
                     /locus_tag="PC407_001239"
     CDS             15796..16707
                     /locus_tag="PC407_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2496696.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEVGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            16758..17570
                     /locus_tag="PC407_001240"
     CDS             16758..17570
                     /locus_tag="PC407_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDF2496697.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            17680..18372
                     /locus_tag="PC407_001241"
     CDS             17680..18372
                     /locus_tag="PC407_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2496698.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            18459..19400
                     /locus_tag="PC407_001242"
     CDS             18459..19400
                     /locus_tag="PC407_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496699.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            19437..20594
                     /locus_tag="PC407_001243"
     CDS             19437..20594
                     /locus_tag="PC407_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2496700.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRHFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            20746..21306
                     /locus_tag="PC407_001244"
     CDS             20746..21306
                     /locus_tag="PC407_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2496701.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            21334..21825
                     /locus_tag="PC407_001245"
     CDS             21334..21825
                     /locus_tag="PC407_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496702.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            22054..22605
                     /locus_tag="PC407_001246"
     CDS             22054..22605
                     /locus_tag="PC407_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2496703.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            22691..23623
                     /locus_tag="PC407_001247"
     CDS             22691..23623
                     /locus_tag="PC407_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HDF2496704.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            23678..24541
                     /locus_tag="PC407_001248"
     CDS             23678..24541
                     /locus_tag="PC407_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2496705.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            24788..26650
                     /locus_tag="PC407_001249"
     CDS             24788..26650
                     /locus_tag="PC407_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2496706.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            26653..28119
                     /locus_tag="PC407_001250"
     CDS             26653..28119
                     /locus_tag="PC407_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2496707.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIAKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            28143..28979
                     /locus_tag="PC407_001251"
     CDS             28143..28979
                     /locus_tag="PC407_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2496708.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKKFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT        10753 a         3505 c         4667 g        10177 t
ORIGIN      
        1 ctcatttaga taaaaaattt tagtaaaagt aaaaaaaata atatatttgt taaaaaataa
       61 attgacaaaa tattcagaaa aaaacttctt gacctaagag agtaaaataa ataaaatcta
      121 aggtatagag atgaaatcta taaaattatt ttaagtataa gttaaaaata tagatggtgc
      181 atttaagatt tataaaaatt agagatgcat aattatttta gggaggtttt tagtatggag
      241 aaaaaagaaa aggtagtaga aaaatcaaat gttgaagtat gtagtcctga aattgtcaac
      301 agtgttgaaa cattaagaat gagattagaa gaaattagat tggcacaaaa agaatttgca
      361 acatttacgc aagaacaggt agataagatt ttcttagctg cttcaactgc agctaatcaa
      421 caaagaatac ctctagcaaa aatggcagtg gaagagactg gtatgggtat tgttgaagat
      481 aaggttataa aaaaccattt tgcctcagaa tatatctata atgcttataa agatactaaa
      541 acttgtggtg tcatagaaaa agatgaagca tttggtttta caagaattgc tgagccagta
      601 ggtgttttat cagcagttat accaacaact aatcctacat caactgcaat attcaaatcc
      661 ctaatagcac taaaaactag aaatggtata attttctctc ctcatccaag agctaaaaac
      721 tgtactatag aagcagctag agttgtacat gatgcggcag ttaaggcagg agcaccaaaa
      781 ggacttatag gttgggtaga tgttccttcg attgaattaa ctaatgttgt catggcagag
      841 gcagacctta tattagctac tggtggacct ggaatggtta aatctgcata ctcttcaggt
      901 aaaccagctg ttggagttgg gcctggaaat gtacctgcaa taatagatga gagtgcagat
      961 ataaaaatgg ctgtaagttc aatcttagta tcaaaatcat tcgataatgg tatgatatgt
     1021 gcttccgagc aagctgtaat agtaccagaa aaaatatatg aagaagttaa aaaagaattt
     1081 aaataccgtg gagcacattt cctaaataaa gaagaaactg aaaaagtagg aaaagtagta
     1141 ataattgatg gttcattaaa tgccagaata gttggacaac cagcacatgt aatagccaaa
     1201 atggcggatg tagaagttcc aaaaacagct agaataataa ttggtgaagt agaatctgtt
     1261 gaattaaatg aaccttttgc acatgaaaaa ctttctcctg tacttgcaat gtacaaatct
     1321 aaaagttttg aagatgcagt tgctaaagct gaaaaattag ttgcagatgg tggatatggc
     1381 catacatctt cgttatatgc agactctata aatcatccag atagagtaga gaagtttgta
     1441 aatgctatga aaacttgtag agtattagtt aatactccat cgtcacaagg tggtataggt
     1501 gacttatata actttaaatt agcaccatct cttactcttg gttgtggttc ttggggtgga
     1561 aactcagtat ctgaaaacgt gggagtgaaa catttactta atatcaagac agtagctgaa
     1621 aggagagaga atatgctttg gtttagagca ccagaaaagg tttatttcaa aaaaggttgt
     1681 ttaggtgtag cagcaagaga atttaaagat gttatggata aaaagaaagc ctttatagta
     1741 acagattctt tcctttacaa taatggatac actaagaagc taactgattt gttggatgag
     1801 atgggaatta agcatactac attctttgat gttgctccag acccaacatt agcttgtgca
     1861 agagaaggag ctaaggctat ggctgatttc caaccagatt taataatagc tgtaggtgga
     1921 ggtagcgcaa tggatgctgg taagataatg tgggtaatgt atgagcatcc agaggtagac
     1981 ttccaagatt tggctatgag atttatggat ataagaaaaa gagtatatgt atttcctaag
     2041 atgggtgaaa aagcgtattt tgctgctatc ccaacttcag caggaactgg ttcagaagta
     2101 actccatttg cagttataac tgaccaagat tcaggtgtaa agtacccatt agcagattat
     2161 gaattaatgc caaacatggc tataattgat gctgatatga tgatggagat gccaccaaga
     2221 ttaacagcag catcaggtgt tgatgcttta acacatgcat tagaagcata tgtatctatg
     2281 ttaagaactg aaccagctga cggtctagca ctacaagcag gtaaaataat atttgaatat
     2341 ctacctcgtg catacaaaaa tggaaagaat gataaagaag caagagaaaa aatggcaatg
     2401 gcatctacta tggcaggaat gtcttttgca aatgccttct taggaatatg tcactcttta
     2461 gcacataaat taggtgcatt ccaccatgtt caacacggtg ttgcaaatgc attattaata
     2521 aatgaagtaa ttaagttcaa ttgtgcagaa gctccaaata agatgggtgc attctcacaa
     2581 tacagatatc ctgattgtat acaaagatat gctgaatttg cttcttttgc aggaatcaag
     2641 ggaaatacag accaagaaaa agtagataac cttataaaag caatagatga attaaaggca
     2701 agagtaggac ttcctaaaac tataaaagag gctggagtag aagaatctaa attcttagaa
     2761 acattggatg ttatggttga acaagctttt gatgaccaat gtacaggtgc taatcctaga
     2821 tacccattga tgagtgaatt aaaagagata tatctaaaag tttattatgg taaataatat
     2881 tcattgtaca acataggttc tattgggttt gtatataaat tattagattt gaatataaat
     2941 agagttttat tagagaaacg tagaagtaac tataaataat agattataac ttaaattctg
     3001 aatggaaatg ataaaaagtc agtaatttaa tggtaatttc gaatatgtgt atttaatcaa
     3061 ttaactttaa aattagtcgg aaaatttttt tattacatat aattttaaag cataatatat
     3121 aaaaaacttt atgaaaaaat tataaaaata ttcagaaaat tctattgact aatttattta
     3181 tcatgatata ataaatttag aaaagaaaaa aattggggcc tattaaggcc cccttactat
     3241 tttttgatag atttatttaa aattactttc tcattttgat tttttatatt tattatagag
     3301 tcagtataat ccacttcaaa gttggcaaaa gcagagaata aatctatatc tcttaacaaa
     3361 tgaattgtca tacattgatt gttcttattt atccttagaa tcaatgctct tacaaatgga
     3421 aattgtattc tagaattaaa taagttagaa ccatcagtat ctatatctaa gctacgataa
     3481 ttaatatcta taaatatatc tacatcatca ttttcttctc ttattattct gtagtcaaaa
     3541 tctaagttta aatttaaatt atctaaaatc aataacattt aaatcctctt ttctataaat
     3601 agttttagag tgttaattta agctctccat ctattataga tataattcta tctgcttttc
     3661 tagcaatatt ttcattatgt gttattatta taagtgtttg gttatatttt tttatagaac
     3721 tttttagtaa atccatgact tcttccgtag ttttactatc caaatttcct gttggttcat
     3781 ctgctaggat tatacttggt tttgcagata aagcgcgtgc aatagcaacc ctttgttgtt
     3841 gaccaccaga taattgattt ggaagatgat ttttcctctc actaagaccc aataaattta
     3901 taatgtcttc gatgtattct tcatcaactt ttttattgtc taactttagt ggtaattcta
     3961 tattttcttt tacatttaat actggaatta ggttatactg ttgaaaaaca aagcctactt
     4021 tttgtcttct atattttgct aattttttat ctgaaaggca acatatatct ttaccatcga
     4081 cttttatagt tccactactt ggtctctcaa gacctcctac taaatgtaga aatgtgcttt
     4141 ttccacttcc acttgggcca attatcgcaa caaattcacc cttatttatt tttaaatttg
     4201 catcctttag tgcatgtaca gctgtttcct tttttccata aatcttacct aagttaagaa
     4261 tctctaatat aggcataatt aatcctccta ttctgtaatg tttatagctt caactatact
     4321 aattttattt attttcctta atggaatata tgtagctaga atacctacaa ttatgtttgc
     4381 taaaacaact aggatatata ttttgtaatc tattgaaaat cccaatccat atgatacaaa
     4441 attatacgtg taatacattc taaactgtat tataagccct accacaatgg taattacact
     4501 tgagagtatt ccatataata ttccctcata gagtatcata tttttaaatc ctctctcaga
     4561 gataccaata gctcttaaca ttccaaattc gctagttctt gaagttaagt tataacttat
     4621 gttatttatt atattaaaca cactaattat aaacattact gctactattc cataagcata
     4681 agtcagagcc ctagctgtca tcttatcaaa gttttctttt tccattagca tattagctgt
     4741 tgtagttcca ggagattcac ttcctatttc acctaagagt gtatttatct tttttacatt
     4801 tgcattttta ttaatatcta cataaactac attatattta tctacaccag ttagtttttt
     4861 tagatagtcg ttacttgtta ttacatctat accctgatct ccagaatata ggtaattatc
     4921 tgcaaaagga taacttacaa tagctccaac tttaaactca tattctagat attcataatt
     4981 atcttttagt tttatataaa gatcttgatc tatctcacct tttggatatt tgacctttat
     5041 agtatctcct acttttatat ctacagcagg cgtttctgta ccaggagtat aacttagctt
     5101 gtatttattt gttttagaaa cttgtggtac gtataatata gcgaggtttt cttttttcat
     5161 tctttccaga tttatactcc cactcactaa gtatttttct aatgaattta tcatctcatc
     5221 attgtaacct cttaactttt gtttcattgt atattttcca gtattcttat tttttatgaa
     5281 tagaggaaaa tccttataat aaggcgttga attaataata ttgtagtaat ctaatccatt
     5341 taatttatct acttctattc ttgtatctaa gatgctggca gttttgactt tgtcaattcc
     5401 atttatattt tttattttat ctatctgatt atcattaagt ccttttatat ccttgaaaga
     5461 atcagcttta ttagattgat ttagttttaa gaaaaaattt gttagaataa aatctgcatt
     5521 catatatgtt tcttcttgac catttttatt ttgtatatct gaaaaagaat atttataatt
     5581 ctcctttatg atcattatac ctcctagact catggataat ataattataa tgaagccctt
     5641 tttattccta aagatatttt tagcagatat agattttgtt actgatatat ttaaagttaa
     5701 ttttgatatc aaagaattga ccttatttat atttttatca gttccaaagg tttttcttat
     5761 agagtcaata ggagaaattc ttcttatctt taaatatgta aagaaactta taattagtat
     5821 tgtcagtatt atacttccaa ttgataaaag tattatttta tctggaataa caaaaggggt
     5881 aactgttaca ttaccttcaa attgaatatt tcctgcagtc ctattaaata tttgtgcacc
     5941 accaatacct gtgcatatac cgataggtat tgctatcaaa gcaagtatga aaagttcata
     6001 aaacataaac ttgaaaatct tgaagttagt ggatccaata gctcttaaga taccatattc
     6061 ctgtattctt tgatatatcg atataacata gatactatat attacaatac cagaaaatag
     6121 gctcatagta attgcagtgt atttacttga gttatctaaa gtaccatttt ccctagtaga
     6181 ttctataagc attttgtttt ttccaacact attctcatcc aacatagtat tttttactat
     6241 attatctatg cttgtattta tatcaatgcc attattaaat tcaatataag cataagaaaa
     6301 tttatccaat tttgattcat tgagatttaa aaaaatttca catgtccctt tatttttctc
     6361 aataggtcta tcttcaagta ttcctacaac tttaaatgtc tctggttttt ctttttgata
     6421 taatttaaaa gtaatgttct gatttatttc tggttttaaa cccagactat ttaaaatcca
     6481 tttctctacg accacctcat tactagcttt tggaaggtga ccttttataa gtttggattg
     6541 attagtcaag taatttttat ttgcatattc taggtttata gctaatcttt cattaggatc
     6601 tgtatagcca tagtgagatg ttatacctaa atttttaata tcttttctag actttatgta
     6661 ttctaattga tttttattta tatctttaaa gtatacatga tcacttccag tttctctttt
     6721 taatttatct acctctgctt gttgagcact tcttgagagg gtaccaatac caacaataag
     6781 tgatgtagca agaattatac ttagagcaat tgctaaagac cttgctttat aacttattat
     6841 gtatcttagt gctattttta aattatcttt catatttacc acttcctttc ttagctaaat
     6901 caattatatg tttatttaaa atatatatca aagtaagtat cttcaagttt tgagtactta
     6961 attgtacaaa tttatatgtg tatttgcaag tttaattaat gtacatgtaa attttgactt
     7021 tttacataat ttttctaaaa tgatataatc ctcatataaa gtgtagatgg gaggggaatt
     7081 atggagaata attttatatt tcctatagta atactaactt tgtttatatt tagtaatctt
     7141 ataatgaata aatacaggtt ttataaagaa ttagacatat taaaaagagg aatattaacg
     7201 ttttttgtat cgtgttttgt atggatttta ttcatggagg gtattgagta tatacaatta
     7261 agtttaggat ataatttttt acttgaagga gtattaaata ttatactttt tttaatttta
     7321 attattttaa gcttttatat aggatttaaa tttataagat tactagacaa taacaataaa
     7381 caagagtata agctacaaaa attagacagt aaaataaatc ataaggacaa tataatcgta
     7441 aaattaagaa gtcaaaaaca tgactattta aaacatttac aagtagtata ttctctatta
     7501 aatactgggt tagctgaaga tgcaaaagaa tatataacga atatatcaaa tagctttaaa
     7561 tcaaaaaatt caagatatgg gaaagtatct tatttagatg ctatagcttc cttaaaatat
     7621 gaggaatgtc ttgaaaagaa catatctttt gatgtatata tccaagattt tatggataat
     7681 ttagagattg aacctaatga tatgagttca gtgctattaa acataataga caatgcaata
     7741 gaatctttgt ctgctataca aaaggaaaag aagtatataa gattacatgc atatgaagat
     7801 gaatttcagt atattatagc cataaaaaat aatggtccta aaatacataa tgtcgaaagt
     7861 atatttttgg atggatttag cactaaagaa gggcaagaca gaggttttgg gttatatcta
     7921 gttaaaaata tactagataa gtatgattat agtatacttg taaatagtga cgatttttta
     7981 actgaatttg taattttaat accaaaatat tattagttag gaggagagaa gtctgtgata
     8041 aaagttttga tttgtgatga tgataaaatt gtaagagatg atatagcaaa tatagttaaa
     8101 gagtccggat atgtagatta tgttaagaaa gtcaaggatg gtatagaagc aattgaattt
     8161 ataaaaagtg agaaaataga cttattgatt attgacattg atatgccata taaaaatggg
     8221 attgattctg ccaaagaaat tctttctata gataaagata tacatatagt ttttgtaaca
     8281 ggttttgctg actatagtct tgagagcttt aaagtacatc ctatagattt tatagttaag
     8341 ccgtttaaaa aagaaaaaat acttgattca attaatatag caatagacca cattaactct
     8401 cataaaattg caaaaaataa ttttatagaa gatactcttt ttgtatataa aataaggaaa
     8461 cagatacata tgattaattt tgatgatata gtcatgtttg agaaaaattc aagagctatt
     8521 aatttatata ctagagatga agatacgata aaattttatg agaactttga tgatttaaaa
     8581 aaacgcctac ctcctaattt ctttatgact catagacaat acatagtaaa tttaagactt
     8641 atacataagg ttatacctat aaataagtct tctttagaga taaaatttat aaattttcaa
     8701 gaaagtgcta ctttaaataa atctttagaa aaagattttc tatacagatt ttatagggtt
     8761 aaaaggtttt aatgaagaaa aaaattttca gtgatataca atatgtatga tgaagatatc
     8821 atataaattt gctatagtta taaattaatt cgtataaaat ataatataaa acatattgga
     8881 ctttcatgga aaattagaaa gcccaatatg ttttagagat aaatcttata tttataagtt
     8941 gcttaagata ctagggtctt taatcttatt atcttcagca agtaataaga ttttagacat
     9001 aatctcagca gtcttagggt cttcatcagc gaatggaagg aatatcttac ctctactttg
     9061 agaatgtaca ggaagtatat tcaaagcacc catccctttt ttatgtacaa taccagaacc
     9121 catatgcaca gaatactcac ctaaattacc tgtgataaga acatgagagc cttcaaaatt
     9181 gatattgtta atttttaata ataataaaag ttctttagca attgctatac gcatctctac
     9241 agtagaatgg ctagtttcag ggtcaacacc accagcatga gcgacactta caacaaggtc
     9301 tatatcacgc ataacttctg agaatagaat tggaggtata gaagctaatt ctaaactttt
     9361 ataagttttt ctatcaaaaa attctactgt ttcaatagta ggaggctcta tatctgctgg
     9421 tgaaaaccaa tctgcaagtg caaacatctt tgccactata ttttctttat aaaatacttt
     9481 ctgtaatccc tcttcataat cgacagtcca aagctgattt ctaagaagtg ctacagtttt
     9541 ctttggctga atttggtgac cagcatatct gcgagatata gttttttcag atatttcatc
     9601 agaatttatt ctataatact cacggaatac ttgcttaaat ggttgaacaa gttgcttact
     9661 aaataaatca tgttggaact cactccactt accagattca aacaaatgaa ctggatgtgc
     9721 aagttttaat ttattttttg ctgtcaactt ataagtttca ccttctatat tgcaaataaa
     9781 gaacttagac ttatcattat aaacatatcc aatattatca tcacataccc atactagatt
     9841 tttaatcaaa gggcagataa ctggattttt atagatattg gcaatttctt taactgtaaa
     9901 tactatttca tttttcatag cttgttctaa actctgtttt gcacgagaat actgactctt
     9961 taaatctttt tggacagatt taagttcaag tacatatgga tgtttagcaa tagcttttgg
    10021 tatactcttt aaagacttgt tgcctttttg aactttagta gatgcttttc cgctttcatc
    10081 aacatctatc caaattgaag taccatcaat atcttttggt ttaaatagat gagcaacttc
    10141 atttagtttt tcagtctcaa gagcccacat catacgattt acatctccaa atccagcact
    10201 tctagctaga ttttctagcg ctatagtaca agcttttgat tcactttcac gacgttgtgc
    10261 accaaattgc ttgctttctt tagcaaactt ttgaataaat tcataacgga aagttgcttc
    10321 ttttaggtct ccttttttca taggaagtaa agagaaactt ctcaatttat cttgatttct
    10381 atttgtttca atttctttca tcaacatgtc tttatcaagc ttaccaagtg tagcatcagc
    10441 atagagttgt gcacgtctat gagagttacc accactagtt atatatttag ctgacttgta
    10501 aagtatattg aatctttctt ccccaatagt tttatatgaa tctaagaacc aatctacatc
    10561 aaatgcacca tcaatgaaac tacgtggtga aataggggag aatagagcaa cctctgattc
    10621 tttttgtgca gagaagcctt catttatatg tgcatggaag taccaaattg tacttttcag
    10681 acccttccag tttaaatatt tttcaattat gtttacccat tgaggggcat acatagcaga
    10741 ttctattaat cttaactcag taatgtcggt atttttaagt aaacttttta atttttttga
    10801 atcatcttct ttagtaggat agcaattttt aagtagagaa cttaaaacat ctttttttga
    10861 ataattgcta ccatatgaat atcctctctg gaaagtttct ttaccaagag cgaccaaaat
    10921 atttacaaaa taatctatac cttcaaagta ttgtatatta ttagctagat gagacacttc
    10981 tgttggcata tcacctctac gtgattcaat ttctataatt cggtttgtaa cagtttttaa
    11041 attatcttta agccaagggt acttttctaa tgtgtcacca gcccatctag gatttgttaa
    11101 tgttcttatt atatttgatg cgttgtatcc acttatcatt tctctattaa cttgctcttc
    11161 agtcattaaa cctaattcat gtgcacgagc aaacacattt atgctagtat agaaaggaaa
    11221 cttatattct agcaactgat aatttgaaaa cgtagtgtag aaaaacttag taaaagaaat
    11281 atcatcataa ataaacgaat gtgctttatc aagccaatag tttaaaatag gggcatttaa
    11341 agcagggcag tattcatttt gtctgtaata gtttttagta aattcaactc tttgtttttt
    11401 acattgttct atagtcaata agtttctaag tttatagtat atttctaaac aatagttaaa
    11461 tatttcttta tggtcaaact catttagtaa aactcttaaa agttttcttg taagtccatt
    11521 aaatttaaat ttttcaagtt ttgtatatat tttattttgt tttttagagt caaggacatt
    11581 tgaatatata gatgaaaacc aacttttttc tgtttcatta tatccacatg tacttatact
    11641 ataaaataag atatcaagta ttaatttgta atctaagtca agctttttac tagtttctct
    11701 ccaaagttct gccaatggat aattatcaag agtatcatta gaaactttat atttaatagg
    11761 agtcaagtta taagtagaat aacctaaaag cactttgtct acattacctg accatctttc
    11821 aacttcatat tcataatctg cattttcagt aataagctca tcaaattttt cataaacttt
    11881 tattatttta tttatatccg tatcgagaat tttttttgct tctttttttg ttgctacaga
    11941 aacagaatcc tcactagtag ataaacttat atttgaaata attttattaa gctttgatga
    12001 gctattagaa tcatctttaa aatcaaattg ttctggaatt tgaactttgt tttcagggtt
    12061 atatattcca aaaccatttt ctttgcagta atcatttttt gctttaccta acaaattatc
    12121 aattaatatt tgaacttgag atgtattctt tttagattta tcaatatcct ctataagtaa
    12181 aataagctta ttatatagtt cttgtctatc agaattgtct tttaataagt ttacaatttc
    12241 aagacctcca aggcattttt gagaatttga agattttatc agtgaagaag caatattttc
    12301 caaatttgaa tcatcttgtt caagtaaaat ttttattact gtttgtctaa tagagcttgt
    12361 ctttaaagac aatagattgg ttatcttatc taattctaca tctgttagct caagctttct
    12421 aagtaaactc aataccgttt ctctattgga aacactctta tcacttaaac attccaataa
    12481 aaattcacgt tgtctatctg tctttggttc atttacaaaa ttgactataa gtgaagctct
    12541 taaatctgaa ctcataagat gacaattttc cataagcaca tctataaatt gagcattcat
    12601 atcgtatgct gttaaagtca tcatacattt aagtatttca tgagaagaaa tttcaatcca
    12661 agtccatgga aatacacttc ctgaaaatga ttttgtcttt gaatttaaaa tttctaaagt
    12721 atgttttaaa ctttcaaatt gttcttttct agcatcaaag tctttgtcaa taggagtgtt
    12781 tgacaagtca aaattagttt ctctgttttg ccaactatag tagtagccaa cagaaccttc
    12841 tacatagaaa tttgtaatta cccatgctag tatctctaaa tcatcaccct taatgttttt
    12901 agatgctata gaatgtttaa aatatgtact ttctgtatgc gataaaaagt aaagagctac
    12961 aagtttttta tatttttcct gacaagaaag taagttttca ataagagagt aagagtcata
    13021 tatgttgtaa gttgcagtag cccataaggc aaaataaatt tcaagattat cattgctatt
    13081 aattgattct atacggaact tttcatcagt caatgattta tatgaaagtt ctaaacattt
    13141 acgtattatt ccaggttttt ggtctgaaaa accaagtcct gtccatgtat ccaaggctct
    13201 tataacagaa ctaaatcgct ctaaattgtt gtccaaaata gtttttaaca tgtatattga
    13261 tgcagaaatt gtaccctcat ctaaactctc tacaatttgc tgtctaagtc cttcttgttg
    13321 tttggctgcc aaaagaagtt tagaaagcat ttcatagcat ttatcactat gactcttcat
    13381 gataccaatt atcatttgtc ttgtaagtag agcagtttga ttttctccaa atattacttc
    13441 ttctaaggca cttgttactt tttgatttcc tttgtctatt tctagtgcta ttttatcact
    13501 cattatagag tcatagtaca agaccttatt tggaagagta agataatcta taactgaaaa
    13561 atcataataa attaaatcaa catagtttga aataaacctt ataatacctc taaaataatc
    13621 cacaagttta tttgagcgat accctttacg ataatatcct cctgaatatg tataatccat
    13681 ctttctttta attaattggc aaagttcttc tacttgttca gaagtaaata ttaattctaa
    13741 tgcatttttt aaaggtccag aaaggagctc gtccatattt ttaatttcgt agtagtggca
    13801 attgtagtat ttctttatca tatttaagaa tttgtcctca ggttctctat aatagtatga
    13861 actattttct atgcagtccc aaagagattt tgctaagtcg tagtactgtt ttgaagaaga
    13921 tttagtaaaa ttatttttaa aattttcaat tattttagga tttttttcta ctctagtacg
    13981 ttctaacatt tatgtacctc caaattattt attttaaaac attcttccag caagcaaggt
    14041 aagtcttata aaagtaaata tatctccact tttcagattt ataggggaat cgagtttttc
    14101 aataacatct tcatttataa gcactttgta gattccatct tcaaatgcct gtaacgctac
    14161 ttctattgcc ttttctacag attgtttatt ctcattgtaa atagagccaa atccaacttt
    14221 tccaatatca gcttgagatt caatttcttc attagttaag aagtttatta ttgatttttc
    14281 tgggattttc tcattatatt caagaacatt tttagataca atttcagaga tagcttcttt
    14341 taggcaattg atgttgtcag atacatcaaa aggggtaagt tctaacatag gtttgcgttt
    14401 gccaacagcc ttaactctaa aataaatttg cataaaatca cctcgcaaaa tattaattat
    14461 gtatttagaa atttaaaatt tatctatttg ttatattata tcattttgtg aattattaat
    14521 gtgaattatg gaattatttt acaaaaaata gaaaaaaagc tatcttaaag tctaaaaaag
    14581 caataaaaga taacttttaa ctataattat atagaattat aactgtacat ttattaaatt
    14641 ttaattttag aattattttt gataactatc tttttttatt tataaatttt gttcctaaat
    14701 attttttgat atcttcttca ggagaaaagt catcaataag tatttctttc ccagtgtctt
    14761 caaatactac aagaacttca cagtcacctt gcaagaaaaa ttttctaact gtatcttctt
    14821 taacttctct aacttttatt acattctttt tttctttttc aaagtctttt gtgtaataac
    14881 ttcccatcat attaattatt tttgtatatc tattcataaa atacctccat atgcgattaa
    14941 atttaaaaaa tatgttataa taattgtttg tattaactta ataattataa caaggagtaa
    15001 taataatgac tagtataaaa aataaagata ttttaaattg tatagattat tccattaaaa
    15061 ataagctctt tgacaaacta aatgatgtat ataagtcctt gccagtagga aattgttctg
    15121 gatgtggaaa ttgttgtatg gagtcagtag gtataaattt aattgagttt ttgaatatat
    15181 tttgttatct tgaagataga gttgatttaa aaaaaagctg tataagtaaa atattagatt
    15241 attattttga ggagtataca agaaagaacc catgtccatt taaagatact aataatagat
    15301 gtctaatata tgaagttaga ccattaaatt gtaggatgtt tggacattgg aaaaaagagg
    15361 attataataa aaatttagat aatgttattg agaaaaataa taactataga gagttaatga
    15421 aaaagcaata tggatttgaa ataaatgatg aagtagtaaa ttacaggata gattattgtg
    15481 atagatttat tccaaataaa gattatttaa gtaaatctaa gagattaagc ttttttgatg
    15541 agcttatgat attagactca aatatttatt caagtggaaa tatagatata gactttaggg
    15601 atagaggtat agttgaatat tttatagagt cattacttta tagaaattta gcatataatg
    15661 tgaaaataag gatatctaaa gaatacaaaa taagaaaaag aactatagat agaataaaaa
    15721 gaattgttct tgtatagttt tatattagta agtaataaat tgtatcataa atattgtcaa
    15781 aatgaggtga ggaaggtgaa tcttacaaaa gtaaaaggaa ataccttttt tataaaaggt
    15841 ggcacgaata caggaatata tgtatttgaa gataacacag cacttatgat agacccaggg
    15901 ttatgtggtt caagacctag gagagtaaat aaagtattag atgaaaataa tatacaggtt
    15961 agatatatta taaatactca tggtcatgat gaccactatg gagcttgtaa tcaatttaaa
    16021 gaatattata aagatgtgtg tataatgtca tctgaatacg acaaattata tatagaaaat
    16081 ccagaattgt tttcaaagta tattgttggg ggaaagagca atgtatttat ggataatatc
    16141 ttaaaaaata aaatgttgga tgatataaag atagataaat ctatagaagt tggaaaaatt
    16201 gaattaaaca atgaaatgtt tgatataatt gagtttcagg gtcatacacc tggtagtata
    16261 gctgttttga cgaaggataa tgtgattttt gttggcgatt tattgatagg agatgaaatt
    16321 ctatcaaaat acgatttctt gtttctatat gatatacaag aacaaataaa ttcattagaa
    16381 aaattaaaaa atatagattt tgagtactta gttttagggc atggcaagca agttatatcg
    16441 aaagaagatt cttataaatt aatagaaaaa catttgaatg ctttaaaaaa atatttatat
    16501 caaataagag agttgcttgt agaaccgaaa tctttagaaa ttttattaaa aaacattata
    16561 aataacaata atttaagcaa taattataaa gagtatcatt tttttaaatc ttcgttagta
    16621 tctgtcataa gttacttagt tgatttagaa gaaatagggt atatattgga aaatggagaa
    16681 ttgctttact atacaaaaac aaaataaatt atgataaaat aaagaaggtc ataaaaaaca
    16741 aataaagggt gagcaatatg gaatacgaaa aatggaatga gatactagca ccttatgaac
    16801 atgcagtaga ggaattgaaa ataaaattta aaaatataag aaaagaatat ttagcaaaag
    16861 gtgaatattc tcccatagag tttgtcacag gcagaacaaa aaaaatatct tccataatat
    16921 ctaagttaaa aaggataaat gcaaaagata tagaaactga aattgatgat attgctggta
    16981 taagaataat gtgccagttt gttgaggata tatatgctat agttgatttg ataaaagtaa
    17041 gaaatgatat gactataata ggagaaaaag attatattac aaattacaaa gatagtggtt
    17101 atagaagcta tcatgttata ataaaatacc ctataaactc cattgcgggt tcaaaggaaa
    17161 ttatatgtga aatacaaata agaactcttg caatgaattt ctgggctaca atagaacatt
    17221 cattaaaata taaatatgac cattacatac ctgagactct tgctgaaagg cttagaagag
    17281 catctgatgc tgcctttctt ttagaccaag aaatgagtga aataagagag gacataatga
    17341 aagctcaggc tatgtaccaa atgaagtcta tagctattag agatactcta aatagaatac
    17401 aagagttata tgacttgggt gatacacata aagctatgca gtaccaaaga aaacttgata
    17461 gaactgaaac agataaaaat ataactgaaa ttttggaatt aaaacaagaa atagatatat
    17521 tactagagca atataaggat actaaggtag atgataaagt agttcagtaa aaaactactt
    17581 gtagaaaatt tgctaaatat taatggggct gcctcttaat ggaaaataat ttctaaaaag
    17641 agacagccta tttagtgtaa ttaagaaagg aatgatatta tgagtttgta tgctataggt
    17701 gatttacatt tttcaacatc agtcaataag ccaatgaata tctttggaag taattgggat
    17761 ggtcatgaaa agaaaattat agataattgg aaagaagttg ttaaggaaga agatatggtc
    17821 ttagtactag gagatacatc ttggggaatt aatttatcgg aagctaaaaa agatttagat
    17881 ataataagca aacttccagg tcagaaaata cttataaaag gaaatcatga ttactggtgg
    17941 acaaccgtta caagtttaaa taagctatat gaagacatgc gttttattca gacaaatttt
    18001 tatgaatata aagattatgc aatttgtgga ggtaggggct ggatatgtcc taatgatgtt
    18061 aaatttgatg agactgatga aaaagtttat aaaagagaag agcatagact tagactatca
    18121 ttagaatctg ctcgtaaaag tggtcactca aaaataattg tgattactca ttatcctcct
    18181 acgaatgata aattggaaga atctttattt acaaagttat ttgaagaata taatgttgaa
    18241 aaggtgattt atggtcactt gcatggtaaa gaatctttta aaatgggatt gaaggggatt
    18301 cgaaatggag tagaatatac attagcatct tgtgactata cagaatttaa tttaataaaa
    18361 gttcacgatt aaaaaacata aattggtaaa tactataaga tacatgatta aaaataaata
    18421 tttgagggat ttcggttttg agaaaggaga cacgacttat gaggtggata ttagttttta
    18481 tatttttata tcttattcct ttagtagtga tattcagaaa ttacaagaat tttaagaggt
    18541 cttgtatcta cagtagtatt tatgtggtat tagctacaac aataatgata agtaatattt
    18601 atatgagtgg acttaataaa ataaaagagt ctatgtatta tcacaattat atttctgatg
    18661 agacatatga tgaaaagtat gtatcaaatt ttaaggaaaa agaaagtacc gatttaaaac
    18721 aggataataa aaagaatgaa gaaatatcaa ataataaaaa tttaaacaac aaacaagata
    18781 gaaatataca aaataaagaa agtaatgaag aaggaagtaa tgatataaaa gaaaagaata
    18841 attctgatag taatgtttca gtgaataata aatcagaaaa acaagaagat aatacaaaag
    18901 aaaataataa atatacaagt aaagaaaaaa atagtgaaga tataaaaaaa atagatatgg
    18961 aaagtatagg aatttttaag aaagaaattt ataatataga aagagcagct ttagttccaa
    19021 tgagagaatg tataccttac actaaggata tagcaaaaaa tataactaaa ttatctagta
    19081 taaaaaaaga tgttacaaat gccagaaaca agtgtaaaga agttgtaaaa gaatatgaaa
    19141 atatgcagat tccagaactt tctaaggaga agtatacaga aacattgagt aatgctagag
    19201 atgacgtaaa gaaagcatat gagcttagag aaaaatctat ggaaagtgct ttgaaattaa
    19261 taaatactaa aagccctaaa tatataggga agataacaga atatcttgat ttatcagata
    19321 agcagataga aagttttaaa aatagattga acgatttaaa agaagaaata aataattcag
    19381 aaacaaataa tattaattaa taagatttaa agatataagt taaaagggag attatgatga
    19441 ataaacagag tataggaaaa ctaaatatgt tttttatggg gattactcaa agaaaccttg
    19501 ataataggca tttttttatt gagttagagg tgatttttaa atctggaaga aaagagttta
    19561 aaggaattgc aatagaggaa gataataaat ataaatttaa ttttaaagga aaatcagatt
    19621 tatatacgtt taatgagttg cttaagaata tttctaaaga ggcagaaaat tatgatggat
    19681 tagtatttaa atatgttgag cgaggaacta ttgttgtaat tgaaggagac aataagaagg
    19741 taaatgtaaa atatttagat aataaagaag aagtacctaa aatagatgaa tttacggcat
    19801 ctcaaataaa aaacagagat tattatgtaa aagtaggaca agcaaatgca cttttaaaag
    19861 aaattggagt acttacaaaa gatggtaaga taaaaaatga taaaataaga aaatacaatc
    19921 agatagacca ttttgttgaa ttgatagata gtattttaaa agaaataaag gataaagact
    19981 gtataactat cttggattgt gcttgtggaa aatcttactt aagctttgtc ttaaattttt
    20041 atataaaaga agtattgaag aaaaactgtt attttatagg aatagattat tcagatgtag
    20101 ttatagaagc atcaaaaaat atggctaaaa acttgggtta taaaaatatg tcatttataa
    20161 aagaggattt gactaattat acacctaata gggatgttga ccttgtaata agtctacatg
    20221 cttgtgatac agctacagat atggcaatag gtctaggaat tagagcaaaa tctgaagcta
    20281 tagtagtagt accatgttgt cataaggagt tattagggca atatagatat gaagctatgg
    20341 aaccaatatt aaaacatggt gtatttaaag ctagatttgc agatttgata actgatggtt
    20401 taagaacatt gttattggag ggcaatggat atgatacttc tgtagtagaa tacatatctc
    20461 cacttgatac accaaaaaat ttaatgataa gagcgataaa aactaaaacg aataatgaca
    20521 aagcattaaa agaatataaa gaactgaaat cacaatttgg agtggaacca accttagaaa
    20581 aacttatata ttagttaaaa atgtaaaatt tacgaaaagc ttgcttaaat atgcaagctt
    20641 tttggtatta tataggtata gtcatatcca gagttttgat ttataattaa agctgtgcta
    20701 taatatttga ttaatcaaat caataaatgt tacatggagg taaaaatgaa caaattgaat
    20761 atatgtattg atatagatgg aacaataaca agcccttatc attttcttcc atacctaaat
    20821 gatatcttta ataaaaatat aactgaagca gagtgtataa cacataattg ggaagaattg
    20881 tatggttcgg gaataagaga tgtgtatgcg gagtttaata ataagtatat acactcatat
    20941 gatgaagcaa agattgtaga gggtgctaca gaggtaatta gcacattatc taaaggacat
    21001 aatttatctt ttgtaacggc tagacatgag tgtttaactg atgtaactaa aaattggcta
    21061 tcgagacaag gattttcaga tatagatgtt tacttattag gaagtgatta taaagttgaa
    21121 aaggcaagag aactgagttg tgacatattt atagaagata atcctttaaa ctcagtgcaa
    21181 ctagctgatg atggtgttaa agtaatactt ctggatacta actataataa agatgttaaa
    21241 catcaaaata tagttagagt tagtaattgg atagatattg aaaagattat aaaacaaggt
    21301 atttaaagat ttgtagattg aaagggggag catatgatag atttaaaaat tgaaaataga
    21361 aaaggaaaaa tccatgtaaa gagcagtgaa ataaaagaca ttttagaatt acgtccagac
    21421 ttcgaatatg tacaagatat atcaaatact ataaatcaag aaaatatatt ggtatttgat
    21481 tgccagttga caggaaattc ttttgatatg gatgatttag atatagaaga aatattggaa
    21541 gagttaggtg aagaaataga tgagtcttat ttcagtgttt tatttgaaga tgtaagagcg
    21601 tatttaaaag atgctactga tgagatagaa gcagacttac aagataatta cttatttgat
    21661 aatttgagat gttattttga tatatacaat ataaatcaag aatttacaga ttttaagttt
    21721 gtatttttag tttcttttaa agatataaaa atttcttcat tagcaaacct agctaagatt
    21781 gtttctaaga gacagcttat aggtgcatct aagttttata gttaaaatga aatattatta
    21841 taataaaagc tagtagcctt gtaaaaatat aggttactag ttttttattt gttcactttc
    21901 acaaaatttc agaaaactat ttacattcta aaaaaagtaa tgtatattaa acataatgta
    21961 cagtataatg gataaaaagt ttctattaaa ttactaagtt tatttaataa tcaaaataat
    22021 ttatcttata ttagtatgta ttagggggta tttatgcaaa gtttaaatga aataatcgct
    22081 gaaaatttaa aaaagataag gaaagaaaaa catcttagtt tagacaagat tgcacaactt
    22141 agtggtgtaa gtaagagtat gctatctcaa attgaaaagt gtgaggtaaa tcctactata
    22201 tctactttga agaaaataac taatggacta aaaatctcat ttacatcatt gatggagagg
    22261 caggaaagtg acatagaatt gattcaaaaa agtgatatag accattttat tgaagataat
    22321 ggaaagtata taagttatcc aatatttcca tttgactcca aaagaagatt tgagatattt
    22381 atgattgaaa tagaagaagg tggaaattta gattcaaatg ctgaacttcc aggaacacaa
    22441 gagtttataa ctgtttttag tggcgaagta accattaaaa taaatggaga agattacatt
    22501 gtatctagtg gaaattctat aagatttaaa gctgatgtat cacatatata tagaaactca
    22561 ggcaaaggta tagctaaaat gagtatggta gtatattata tataaataat ttaatggaat
    22621 tagagtgata tttagatgat tttaggtatg aagatatatt atatttaagt tataggagtg
    22681 tgatttatgg atgaataata aaaaagcagt ttttttcttt tgtagtgatt taaagaaaga
    22741 cccagtagcc agtaatgtga taaagtgttg tgaagaaata atggatttaa atcaaactga
    22801 tattgttatt gatgataatt ttgtattaga atttaaggat aaaaacaata acttatttca
    22861 ttttgtaaaa acaaaagatg taataagtca taattataaa tattatcttc caattttaaa
    22921 tagacatttc agagattatg attttgcagg agttgtaaat tggcatgagg gagaaaatgc
    22981 tccagaacat atcctaacag ctcatacaat tggagatgtt ccaacaggag aatttggaaa
    23041 ttcaaatcca agatacttta aaaatctaat caatgctatt gaggatataa aaaatgaaaa
    23101 tctacttgat gaatttacta cattaacaga agcaactcac tggtctggaa caacatatgg
    23161 tgaagaatct aaattaatta cagaatattc tgtacctatg ttggatattg agataggaag
    23221 tagtagtgat agttttaata atagtatagc aatacaggta ttggcaaaat cattaattag
    23281 agtatttgat tgtgatgaac ctttgaagac tttattatgt gttggagggg tacattttga
    23341 gaaatctttc tcagatatta taaaaaataa agaatataac atatcaattg gacatgtatt
    23401 gccaaatcaa tggattgtaa gtggaatgta tgatgatgaa tcgggatttg agaaactaga
    23461 aaagtgtata aatagtattg aaggtggaat tgattgtata gtttttcatg ataaattaaa
    23521 aggtacctat aaagagcaat gccgtaaact aggtgaaaaa cttaatgtac ctgtatttaa
    23581 acataaaata ttaaaaaatc ccaaagactt acctatatgg tagtcttata cagttaatat
    23641 aacaataagt cggattaatt tttaagcgag gtcaaaaatg atattatctt attttgcatt
    23701 attaattaga gtagtgctat tagcttttga aagagtagtt gtaagattat taggtgatga
    23761 agaaggagat atttataaaa atatagcttc atcatttcta tttttcttta tagggggagt
    23821 atgtttactt ccatttagca taatggatag agttagtgat tggtcgtttt taataccatg
    23881 ttatataagt agtttagtct attcaattgg ttcagtagcg tatgtaacgt ctttagcaac
    23941 tggggaagtt tcacttgtta ctccaataaa tagtttaaat tcattgtttt tactgctttt
    24001 atcagttata tttttaggtg aaggtttaag tttagcaaaa gttgcaggaa tcataattat
    24061 gattggtgga gtgtttatat taaaaaaagt aagctctttt ttaaagaatg tctctgtaat
    24121 ttttaacaat ttaccatgta gattaatgtt tttatacata tttttgcagt cagtaggaag
    24181 agtattggat aaggcttttt ttgtacaagt atctcctata ttatattcaa ctatactgta
    24241 ttttttcgta ggtttaaatt tatttatctt tttagcattt aaaaagaaac aaaaaataat
    24301 ttgtgagatt tttagtaata aaaaggggct ttcaatttta agtggtgcta ttaatggata
    24361 ttcttattta gcattattaa ttgctttaaa taatctagaa cttagtatag cagagccatt
    24421 ttctcaagta tctatgataa taactttaat acttgctcat tttatattca aagaaaatat
    24481 aaaagaaaaa ataccaggtt caattttgat actaattggt gggtggctac tgttacttta
    24541 attgacataa aaaatgaatt ataaaggtgc tattagttag tatcagaaaa tttaaaaaag
    24601 tattgacaat tcttttaggc aaatgttaat ataataatac aaaatagtat aaatagaaga
    24661 catgttactg gtaatgcagg caggatcgaa tatattgctt tgatttttca aggtgtttat
    24721 ataggttctg ccttttttgc atcttgaatg gccattcata aaaaattatg gagaaggaga
    24781 ttacaagatg aattataaaa aaactgccat tgacattatt gaacaagttg gtggttcaaa
    24841 aaatatttta gctttagaac actgttcaac tagactacgc tttaagttga ttgatagaag
    24901 taaagtcaat caggatgctt taaaaaaagt acaaggtgtt atgagtatag ttaatggggc
    24961 acaatttcaa gttgtcattg gaaatagtgt tgttgatgtg tatgatgaaa ttttaaaagt
    25021 ttgcccttta gatacaaatg atagtactgt aaaaactgaa gaaaaaataa acttaggaac
    25081 aagatttata gaatttatta ttaatatttt tcaaccactg atttttgcta ttgcaggagc
    25141 tggtatttta aaatcactgt tgatgttatt agcaatgttt aatattttaa aatcagattc
    25201 tactatatat acattattag ttgctatttc agatgcaaca ttctatttcc tacctcttat
    25261 ggtagctgta actactgcca atgtattgaa atgtaatcgt ttagtagcta ttgccgctgt
    25321 aggatactta ttgcttccag caacaacaac tgctttaagt gaaggtgtcg aactatttgg
    25381 atttacaatt ccaaacattg cctataatgc acaagttttc ccggctatct tatgtgtgtc
    25441 ttttttggca acaatggaga aaatttttaa caagtattca ccaaaaccaa ttcgaacttt
    25501 ctttgttcct atgatgtctt tggcaattac tgttccaatt actctaacta ttttaggtcc
    25561 tttaggatat aacgttggta caatttttac aaccattatt ttattcttat ataacaaaat
    25621 gggattctta gttgtaggat tacttgctgc ttttcttcca tttatggttg caacaggaat
    25681 gcacaaagca ctaatacctt attcaattaa tacaattgga aagttgggtt atgaagcttt
    25741 atatatgaca tcttcattag cccacaatat ttctgaaagt ggtgcttgtt ttgcagtagc
    25801 cttgcgaaca aaagatgaaa cattaaaaca aacagcatta tctgcaggaa tttctgcctt
    25861 gatgggaatt acagagccag ctttatatgg tattacttta caacataaaa gagcaatgtt
    25921 aggtgttgta ttctcaagtg caatttctgg tacattttta ggattatttg cagtaaaagg
    25981 atttgtaatt gttggaccag ggcttgccag tatgagtatg tttattgacc caaacaatgg
    26041 aaacaatttg atttttgcga gtattggttt tgttattgca atattaggct cgtttattat
    26101 tacactgatt atttggaagg aagatacaaa tacagtagag tcatcaattg gagatgttgt
    26161 tgaagaagag ctagaagaag aaaaagaagt aaatatctta gtaactccag tagaaggaaa
    26221 agtaatagat ttaagtaagg ttaatgatga actatttgca agtaagactt tgggagatgg
    26281 agttgcaatt ataccgacca atggaaattt atacgcacct tgtgattcag aagttgtgat
    26341 gttatttgaa acaaaacatg caattggttt aagaacaaaa aatggggctg agattttgat
    26401 tcatattgga ataaatactg taagtatgaa tggagatgga tttaaaacat ttgttaagac
    26461 aggcgataat gtaaaagaag gagatttatt aattcaattt gatttagaca aaatctcaca
    26521 tgctaattta gattcaactg ttatgattgt aaacaataat ggagcagatt atgcttataa
    26581 agtgttaaac cagtcttatg gcaatgtaaa aaaaggaagt atcttgtttg atgtaaaggg
    26641 aggaatataa atatgagaaa gtttccagaa ggatttcttt ggggaggagc tacagctgct
    26701 aatcaatttg aaggtggttg gaatttaggt ggtaaagggt ggtctgtatc agatgtagca
    26761 aaggcacact ttgatgcaga tgtgaaagac tataaatcta ataatgaaat cacaacaaaa
    26821 gatatagaag aaggattagc acatcctgaa gatgaagtaa attatccaaa acgtcatggt
    26881 agtgattttt atcatcacta taaagaagat attgctctaa tggcagaaat gggatttaag
    26941 acatatcgaa tgagtattgc ttggtctcgt atttttccaa atggagatga taaagagcca
    27001 aatgaagaag gtttacagtt ttatgatgat gtatttgatg agttgataag ttatggaatt
    27061 gaaccacttg ttactatgtc acattatgaa ccaccattaa atatcgtttt aaactatgat
    27121 ggatggtatt cacgacaagt tatcaatatg tttgttaggt atgtagaaac tatctgtgaa
    27181 agatataaaa acaaagtgaa atattggctt acatttaatg aagttgattc tatgattcgt
    27241 catccgtaca ctactggtgg tttagttcga gaccgtttta aggataaaaa ctttgaggaa
    27301 gttatttttc aagctatgca tcatcaattt gtagctagtg cccttgcaac aaaaatttgc
    27361 catgagatta ttccaaattc aaaagtaggg tgtatgttaa caaaattaac gtattatcca
    27421 tatacatgta gaccagaaga tgttttggca actcaacaaa aaatgagaag tatttatgca
    27481 tatagtgata cgcaggtttt tggtgagtat ccagtatatt tactttcgta ttttaaaaat
    27541 aataacattc aaatagcaaa agaagaacat gatgatgaaa ttatgaaaaa gtatccagta
    27601 gattttattt cattctctta ctatatgtct tcttgtgagg cagctgatac aactggatta
    27661 gatattacac ctgggaatac gttattagct gtaaaaaatc cttatttaga aatgagtgaa
    27721 tggggttggc aaattgattc aataggtcta cgtatatctt tgattgaatt atatgataga
    27781 tacagaaagc ctttatttat agttgaaaat ggattgggag ctaaagatat tttaactgaa
    27841 gataaaaaag ttcatgacca atatagaatt aattatttaa aagaacactt taaatgtatg
    27901 ttagatgcga ttatcgaaga tggcgttgaa ctttggggat acacttcatg gggttgtatt
    27961 gatttggtca gtgaatcaac gaaacaaatg tcaaaacgtt atggatatat ttatgtggat
    28021 gctgatgatt atggaaaggg gacatataat cgatataaga aagatagttt ttattggtat
    28081 aaaaaagtaa ttgaaaataa tagtatagat tttaaatgac caatagaagg tgagcaaata
    28141 aaatgaacta cataataaag aaagttttga actcgagcgt tgttttagtt catgatgcaa
    28201 agtataatga atttattttg ctagggaaag gaattggata tggtaaaaaa acaggagaat
    28261 atatttgtgg ttccaatgat aatcaaatgt ttgtccctgt tgaaaataca aaatcaaaaa
    28321 aatttttaga attaatggat gatattcctg tcgacatttt aaagataaca caggaaataa
    28381 tagtagaggc agaaaagtta ttgaactcat cttttaataa aaacttatac ttaatattag
    28441 cagaccattt taactttgca attgaaagga tgcgtaaagg aataaaaatt acaaatcgag
    28501 tattttggga aataaaaaat tattatccaa atgagtttaa ggttggaatg gttgcaattt
    28561 atatggtaga ggagagattg ggcattcaat taccagagga agaagcagct aacattgctt
    28621 tccattttgc aaatgctatg gcttcagatg gaaattcata tgatacgatt aagtatgcca
    28681 aagtaattgg agaaataatt aatatttttg ttttttcttt gaatagaacc ttagataaaa
    28741 agagtatgca ttatatgaga tttataacac atataaaatt ttttgtggaa agattttttt
    28801 cagatagtat gttaagcagt ggtgatgatt tattatttaa tcaaatgaaa aggtcaaatc
    28861 caaaagaaat gttaattgca ttaaaagtta gagatttttt agaaaagaaa tatggaaaaa
    28921 agttaacaaa tgaggaaata gctttcttgg ttgtccatat cgcacgagtt aagcaataga
    28981 gtgaattact aatattatct tatacgtcta catggtagaa aattgaaaga aggtaaagta
    29041 tataaagaaa ctgagacata ttattttgtt gttcattatg tttattttat gagtgtatcc
    29101 tg
//
LOCUS       DAMJVX010000029        28015 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMJVX010000029 DAMJVX010000000
VERSION     DAMJVX010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28015
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.031"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            67..696
                     /locus_tag="PC407_001252"
     CDS             67..696
                     /locus_tag="PC407_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496709.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            697..1479
                     /locus_tag="PC407_001253"
     CDS             697..1479
                     /locus_tag="PC407_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2496710.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAINTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1472..2350
                     /locus_tag="PC407_001254"
     CDS             1472..2350
                     /locus_tag="PC407_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2496711.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIIGKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIKIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSEYYI
                     KLKKDILMTMKSNKDI"
     gene            2383..3342
                     /locus_tag="PC407_001255"
     CDS             2383..3342
                     /locus_tag="PC407_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2496712.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDVALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3656..4348
                     /locus_tag="PC407_001256"
     CDS             3656..4348
                     /locus_tag="PC407_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2496713.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4358..5362
                     /locus_tag="PC407_001257"
     CDS             4358..5362
                     /locus_tag="PC407_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2496714.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGIHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKM
                     "
     gene            5610..6461
                     /locus_tag="PC407_001258"
     CDS             5610..6461
                     /locus_tag="PC407_001258"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2496715.1"
                     /translation="MSLDVIFILKSVIIAIVEGFTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            6565..7311
                     /locus_tag="PC407_001259"
     CDS             6565..7311
                     /locus_tag="PC407_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2496716.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7311..9227
                     /locus_tag="PC407_001260"
     CDS             7311..9227
                     /locus_tag="PC407_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2496717.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTYFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLIYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            9506..10423
                     /locus_tag="PC407_001261"
     CDS             9506..10423
                     /locus_tag="PC407_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2496718.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYKSEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSIIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILSEIEKMKKIY"
     gene            10501..11238
                     /gene="cas6"
                     /locus_tag="PC407_001262"
     CDS             10501..11238
                     /gene="cas6"
                     /locus_tag="PC407_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2496719.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KAYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKIVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            11244..13121
                     /gene="cas8a1"
                     /locus_tag="PC407_001263"
     CDS             11244..13121
                     /gene="cas8a1"
                     /locus_tag="PC407_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HDF2496720.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNLIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQIMFNDYLRQIIYFGELADLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKEYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTSINELFERNNSLKAQKSEYSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFTDKYANKKIQSIYDYKLK
                     SNVLDLLLKNRDLKHLTNNILRNKVKNDMESNNKSNISGIDCFRVVQLRALINSYKKG
                     VYKMDSKNLEKNDEKLRIIYYLGCDIHDYFVNKNSKNKIDGVSYKLLNSVKVGNKSDF
                     MDTIIRVFMSAEKQIPAFILDIEIEKDLDFESIGHAFISGLISGKYEGKDKLPNEKEE
                     K"
     gene            13123..14037
                     /gene="cas7i"
                     /locus_tag="PC407_001264"
     CDS             13123..14037
                     /gene="cas7i"
                     /locus_tag="PC407_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HDF2496721.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGLADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            14039..14740
                     /gene="cas5b"
                     /locus_tag="PC407_001265"
     CDS             14039..14740
                     /gene="cas5b"
                     /locus_tag="PC407_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HDF2496722.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            14751..17072
                     /locus_tag="PC407_001266"
     CDS             14751..17072
                     /locus_tag="PC407_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2496723.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQREFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIIGVGEANDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLADLTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFADSDSNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIHENKHTKQIFGKGVLISNEIDQFI"
     gene            17087..17638
                     /gene="cas4"
                     /locus_tag="PC407_001267"
     CDS             17087..17638
                     /gene="cas4"
                     /locus_tag="PC407_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDF2496724.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEMLIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKEKIIENTLININEINMQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            17641..18621
                     /gene="cas1b"
                     /locus_tag="PC407_001268"
     CDS             17641..18621
                     /gene="cas1b"
                     /locus_tag="PC407_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HDF2496725.1"
                     /translation="MYKDYYIISNGNIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNTLMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIVDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            18624..18890
                     /gene="cas2"
                     /locus_tag="PC407_001269"
     CDS             18624..18890
                     /gene="cas2"
                     /locus_tag="PC407_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDF2496726.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITDGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   19074..20691
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=19074..19102
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            20881..21309
                     /locus_tag="PC407_001270"
     CDS             20881..21309
                     /locus_tag="PC407_001270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDF2496727.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIEPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(21765..22544)
                     /locus_tag="PC407_001271"
     CDS             complement(21765..22544)
                     /locus_tag="PC407_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496728.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMSSEVSSIRCCNCDELLEDITLNKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
     gene            22868..23413
                     /locus_tag="PC407_001272"
     CDS             22868..23413
                     /locus_tag="PC407_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDF2496729.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            23445..24581
                     /locus_tag="PC407_001273"
     CDS             23445..24581
                     /locus_tag="PC407_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDF2496730.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CNLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIERADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     PILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKTKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIERYNIFGGALAYGH
                     PYGASGAIITLHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            24585..25976
                     /locus_tag="PC407_001274"
     CDS             24585..25976
                     /locus_tag="PC407_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDF2496731.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEQLGKQIS
                     LGENISILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNLYMYQYKNLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEYTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIRGGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIVNIGGFKVSSAKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            26052..26927
                     /gene="dpsA"
                     /locus_tag="PC407_001275"
     CDS             26052..26927
                     /gene="dpsA"
                     /locus_tag="PC407_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HDF2496732.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEVGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            26930..27520
                     /locus_tag="PC407_001276"
     CDS             26930..27520
                     /locus_tag="PC407_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HDF2496733.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(27728..27928)
                     /locus_tag="PC407_001277"
     CDS             complement(27728..27928)
                     /locus_tag="PC407_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496734.1"
                     /translation="MKKLKTISIFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
BASE COUNT        11251 a         2684 c         4675 g         9405 t
ORIGIN      
        1 aaatttaagt gctcatttaa actattaatt gataataata ttcaatatta aataatagga
       61 ggcgttatga ataaatatct tctaataaat ccagtggcag aaagaatgta tggagaaaag
      121 tttcacctta ttaaagcaaa tttgataaaa aaggggtaca taatagcaga atgtgaacca
      181 cagttagaat atgtaaaaaa gcagtacaaa gagtatacaa aaaagactga aaatacaatg
      241 ttggattgta gatgtccaga aagtataagt cttttaaaaa gaaataattt gataaataat
      301 tttgaagtac ctatgataga accaatatta gtaaggactt gtagggtttt atacgataaa
      361 tatataaaaa gtaaagatga tatgttggta gtaacctgtc catgtacaca attaagagac
      421 tttgcaagtg aaaagtttaa ggataagggt aatgctatgt tttatacatg gaaagagttt
      481 gctgaacaag aaggtgtaaa atcacttgga aaaatagatg aatcaccaat tccattagga
      541 tatttcaaag atacttttga aaaagttctt gaagtatcaa gtgaagacga aatattaagt
      601 gaaatacaaa aaataagaaa tggctttgaa gataagtatg acatagtaga aatgttttat
      661 tgtaaagatg gttgtaataa tggagatggt ttgtaaatgg tcaataaatt taataataag
      721 tttttaaggc tagtaaagag tgttctgata ttttttataa ttcttttatt atggaaaatt
      781 acaaattatc ttggtatttg gagtgattat atattaccat cacccgaaaa ggtatatagt
      841 acatttttaa atatgataag tgatggttca atatttatta atgtatatgc aagtatgaaa
      901 agagttttga taggttttgc aataaatact gctataggtg tgcctttagg gatattcttt
      961 ggaatttata gtggagtata cgaatacttt aaatctctaa taaatttttt aaggaataca
     1021 cctccccttg ctttaatacc aatgcttata ttatggtttg gtataggaga ggaatcaaaa
     1081 attataatta tagttttagc atcctttttc ccaatcttta caagcacatt gaaagggatt
     1141 aaaaattgtg actcaaaact gattgaagtg ggtagagtat ttgagttttc aaagcttcag
     1201 ataatattta aaataataat accaaatgcc attcttgata ttgctgttgg gttaaagtta
     1261 gctcttggat atagctttag agcaattatt ggggcagaac ttgtagcagc ttcatctggt
     1321 cttggatact tgatatctga tggaaaagaa atgtctagaa cagatgttgt tattgtaggt
     1381 ataatcgtaa ttggactttt aggaataatt acagattaca tattttcgat tattgtgaag
     1441 aaggtaagta agggaaagat ggtggaagca tatgaataat ttagtatgtg actctggata
     1501 tataattaga aatttatata aaagttataa agttgacaat gaagaacatg tagttcttag
     1561 tgatatctct ttaaacatag atagaaacca tataacagta attttaggtg aaagtggttg
     1621 tggaaagact accttgctta gagcaattgc atcacttgaa ggtataaata gtggtgagat
     1681 taggtttatt aatgataata aagagtatag acctaatgtt ggttttgttt ttcaggagag
     1741 cagattaatg ccttggttaa acgtaagtga aaatatagct tttcataatc aaagaaaaaa
     1801 tactatttta aacaaggtgc ttagaaaatt taaacacaat aaaagaatta ttggtaaaat
     1861 tataaatgat aattttagaa gcttagaaac aaatgataat ttgaacaaat caaataaata
     1921 taataggaaa agtaatattg atgtagaaaa atatcttaag atgatgaact tgcagaaatt
     1981 taaaaactct tatccaaatg aattgtcagg aggtatggca cagagagtaa gcatagctag
     2041 agctctttct ttcaatccag atatgttgct tatggacgag cctttttcgg cattagatta
     2101 ttttacaagg attgatatgc aaaatgaagt tataaaaata cataagtcaa ctaataaagg
     2161 cgtaatattt gtaactcatg atattgatga agcgttaaag ataggcaaaa aaatagttgt
     2221 tttcacagac gaaagaaaaa taaaagaatt taatatagaa gatggttata atagagattt
     2281 aacttcagag tactatatta aattaaaaaa agatatttta atgacaatga aatcaaataa
     2341 agacatttaa agaaattaat aatattttaa ggagtagagt aaatgaaatt aaaaaagcta
     2401 ttgataggta taacgatatt aacacttgca acagcatctt tagctggatg tgctaaaaaa
     2461 aataatggag aaaaactttc agaaataaat ttaacttatg taaaatcacc attaaatgtt
     2521 ccttctataa ttcagaaaca ggatgattta tttggaaagg aatttaaaaa ggataatata
     2581 aaagttaact ttcacgaaat tactacaggt ccagagcaaa cacaggcttt agctgctgga
     2641 gaaatagact tcttacatgc actaggtggt acgtcagctt taatagctgc atctaatgga
     2701 gtagagttaa agatattaaa cacttatagc aggtcaccaa aaggatttat gattttgact
     2761 aataataatt caataaaatc agcagcagat ttagtaggta agaaggtggc aggtccaaaa
     2821 gggactatat tacatcaggt attgatttca gcacttgata aagaaggatt aagtatggat
     2881 gatgtggaat ttgttaatat gggtattcca gaagcatcag cagctctttc tgatggtagc
     2941 gtagatgttg cattgattgc tggtccagca gctcttaaag ctatgaagtc aggtagtaaa
     3001 cttgtagcaa atggtgaagg tttagtagat ggtataatag taactgctgt tagcacagac
     3061 tttgcagaga aacacccaga aatagttgaa agatttatga aggttgaaaa ggaaacttta
     3121 gaatatgtaa ataataattt tgatgaagct atggaaaaag tagctaaaga agttgattta
     3181 agtcttgaag aaactaaaga attatatgct tggtatgatt ttagtttaga tataacagat
     3241 aaagatatat cttcgcttga agatacacaa gacttcttaa taaaaaataa gttacaagag
     3301 aaaaaagtga atataaaaga attaatctat aatgctaagt agaaactatg ctaaaataat
     3361 actaagtaag aaatataata aaagtttaag ttaccttatt aaggtaactt tttctatttt
     3421 gaattagtaa tttcaattta ttttagaatg attagttgat aataatgatt aattaatcat
     3481 aaaaagatac ttttttatag agttttatta agatgtctaa taatatggtt atacttatga
     3541 agtcagtaat tgtgaagtta ataatatgat taaatttata tattgtaaaa agattataat
     3601 atattaaaat tggactgtta tatgatgagt aaataggtat attaggagga attatatgag
     3661 ttataaagta tatattgtgg aagatgatat atcaataagt acattactta aagattatat
     3721 tgagaaatat ggatttgaag tgattgttga agagaacttt gatgatataa tgattacatt
     3781 tgaaagtttt aagccacact tagttctatt agatgtaaat ttacctaaat ttgatggttt
     3841 ctactggtgt agatgtatta gacaacagtc gaatttacct ataattttta tatcagctag
     3901 agatggcaat gtagaccaaa ttagagcttt agaaagtgga gcagatgatt atataacaaa
     3961 acctttttac tatgatgtag taatggcaaa aataaagagt catataagaa gggtatatgg
     4021 tgaatattca cctaaaatag acgaaagaat agtggagcta gatggattaa agttttatcc
     4081 agaaaggtta gaactagaat taaatggtga aaatctaatt ataactaaaa aagaaggtat
     4141 actgattgaa tgtcttatca agagataccc aaaagtagta agtagagatt atcttttaga
     4201 aaagatatgg gatgatattg agtttgtaga agaaaatact ttaaatgtaa atgttagcag
     4261 gataaggaaa agatttgagg aattaggtat agataatgct gttcagactg tccgagggtt
     4321 aggatataaa ttaaataaga attggtaagg agaaagtatg aagttatttt taaaagatta
     4381 taaaggttat atttttatat attatatagg catagttatg acaattattt actgtaatct
     4441 tatgaagttt attagttttg gagagagttt atatattctt ttatttagtt cttttatatt
     4501 gttatgtttt ttaatatata agtattataa aaatagagaa gtatataatc tgtttgaaaa
     4561 aggaatacat tctttggagg agtcattttt gtatttagga agctcattct tggggagaaa
     4621 tatatcagtt attttaaaac aacaacataa attgtatcaa tcaatgatac aagagcataa
     4681 aaagatacat agtgaacatt taactttcat caaccaatgg attcatcaga tgaaaacacc
     4741 aatatcagtt ataagccttc agcttcaaga ttatgaggga gaagaagtaa ctgaaaatat
     4801 aagaaaagaa atagatagat taaataaagg gttagatatg gctatgcatt ttgcaagggc
     4861 agataatttt cagaaagact ttatagttga aaaagccaaa ttaaatgaaa ttgtatctag
     4921 tattgtaaat gaagaaaaaa ggttatttat aaaaagtgga atgattccta agattcaagt
     4981 agacaaattt acttatgttt atacagatgt aaaatggatg aaatttataa taggtcaatt
     5041 aattacaaat ggagtgaaat attctaaaga ttatagtaac tacctaacta taaaatctga
     5101 agaaaataat aaatatgtaa ttttaagtat aatagatgaa ggaattggaa ttgtttctaa
     5161 agatttaaaa agggtatttg acccattttt tacaggagaa aatggtcgta agtttggaga
     5221 atctacaggt atgggtcttt atctagtaaa aaaggtatgt gatgatttag gtcataaagt
     5281 aagtataaaa tcagaagtag gaaaaggtac agaagtttca atctacttta ataaagatgt
     5341 aaatagtttg ataaagatgt aaattatctg ttacaatagg gtttttagtt aattgtactt
     5401 ttttattata caatttatta aatgaaatat tcatagttaa ataatcaaaa tttaagagag
     5461 tttatttgca aaccttacat agatgtaagg tttgtgtaat gtttgcagat agttaaattt
     5521 tctaatctaa attacaatta atataagaaa taacatgaga gaaataacat aaaaaaagaa
     5581 attttgtaaa aaaggaagga tgtttattga tgagtttaga tgttattttt atattgaaat
     5641 cagtcataat agctatagta gaggggttta cagaatttat tccagtttca tctacaggtc
     5701 atatgatttt ggttggtaat ttaatagatt ttaaggggca atttgctgaa atgtttgagg
     5761 ttgtaataca acttggggct atattagcag tagtagtact ttattggaaa aagataaaag
     5821 atagcgttat agaatttttt aaatttatat ttactggcgg aaaagaagga aagattggtt
     5881 ttagatttgg aatgaatgta attataggtt gtataccatt tgcgattata ggagttctat
     5941 tttatgataa tataaaatca ttgtttaatt tacaatctgt aattatagga tttatagtgg
     6001 gtgggatttt attattagta gtagaaactt tgtttagaaa aaagaatcat tcaacagata
     6061 atatagataa aattactcca atacaagctt taaaggttgg gacattacaa gttttgtctg
     6121 catggccagg tatgtcaagg agtgcatcta ctattatggg tggatggata gcaggactta
     6181 attctcctac agcagctgag ttttctttct ttttagcagt accagctatg gtggcttctt
     6241 caggaaaaga tttatttgaa tttgattatt caataatgac accaacattg tggattgctt
     6301 tggtagttgg gtttatagta gcttttattg tatcaataat agttatggaa aaattcgtga
     6361 attttttaaa gaaaaaacca atgagagtgt ttgctgtata cagaataatc atgggtgtag
     6421 tacttgcagt attagctttt actaacataa ttagtgtata gaaaaaatag atttattatc
     6481 tataaaaatt aaactaagat aaattataat taacaattat atatatgtta tttcaaaaat
     6541 taaattagga aggatagtta aataatgaaa attttaactg taaataattt aagtaaagta
     6601 tatggtaaaa aaataatttt caacgcatta aatgacataa attttagtat agaagatgga
     6661 gaatttgtag gaattatggg tccatctggt agtggaaaaa ctactctttt aaatatgatt
     6721 tctactattg acaagccaac tacaggaact atggaattaa agggaaagaa tcctttattg
     6781 ttaagaggtg aggaacttgc attatttaga agaagagagt taggttttgt atttcaggat
     6841 tttaatttat tggatacatt aactatagga gaaaatatag tattgccatt gacattagat
     6901 aaggtcagtg taaaagagca agatgagaga ttgaatgaag tatctacaat attaggtata
     6961 aaagatttat taggaaaaag aacttttgaa gtatcaggtg gtcaagctca gaggactgct
     7021 attgctagag cattgattaa caatccatca atacttttag cagatgaacc aacaggtaat
     7081 ttagattcaa agtcatcaaa ggttgtaatg gagctatttc aaaagataaa taaagaaaat
     7141 aaagttacta ctatgatggt aactcatgac ccattagcag cgagctactg tagtagaatc
     7201 ttattcataa aagatgggtc tatatataat gaaatatata agggtagtag tagagagcag
     7261 ttttatcaag agataatgga tgtacttaca ttattgggag gcgataacta atgaatataa
     7321 aacagtttgc tattaacaat atttcaagaa atattaaagc gtatttagga tacttagcaa
     7381 gtattgttat atcatcttca ctgctatttt cttttattat gtttacaagt catccagatt
     7441 tagatgtatc tcaatttcct gattatttaa aagaaggtct caagatgagt aagataattg
     7501 catatctatt cttattcttc tttgtgtttt attcagttag tgtgtttctt aaaagtagat
     7561 ataaagaatt tggaatactt tatattacag gtatatcaaa acgccaagta atgaaattaa
     7621 tctttataga aaatatgata ataaatatag tatcttctgt aattggaata ataataggct
     7681 taatctttgc aaagatattt ttagtggtta tgtctacttt tttagaatta tcgccattaa
     7741 aattctatat accatttaat gcgatattgt ctactttaat ctattttatg attttagctg
     7801 tattaacatc aatatttact tattttatag ttaaagaaaa tcaggtgttg aagttactta
     7861 aaggtacaaa aactccaaaa ccagagccta aaacatcact aatgcttgct atattaagta
     7921 tttgtttgtt tataatagcg tattataatg ctattacctc aaccgaccaa tatacaacat
     7981 atctgcgctt agttccagtt acatccctta ctatagtagc aacttacttg ttcttttctc
     8041 aatttagtgt attttttatg aagaagctaa aactaaacaa gagattttat agaaaaaata
     8101 caaatatgct ttggatttcg aacttgattt ataaggtaaa ggataatgct agaatatttt
     8161 tcttgataac tataacttct gcagtagcat ttactgctat aggtacagtt tattcctttt
     8221 ggaaagatgt agagcgtcaa attaatttaa tatatcctaa cacaatatat tattcaacaa
     8281 tgacattgca taatgaccct aaaaagcctg atagcaaaga aaaagacaag gaaagaatga
     8341 tgtttataga aaataatcta aaaaaagaga atgtagacta tacaaggact aatggtgaat
     8401 ttaaaactgt atttcccaag aaaagtgatt ttaatgtcag aattatgaaa gaatctaagt
     8461 acttagaaat tacagaaact ataggggtta agcctataag ttttgaagat aatgaatgca
     8521 tatcattatt atcatcaagt ttaccaggaa ataataacgt caaagaaaat attattgtag
     8581 ggaatagagg tttaaaggtg gcaaaacaag tggaagaatg tgttatgcca gcatattaca
     8641 aaaatatgta tgtggtaaag gataattttt atgatagtat agaatcaaaa tttatagtgg
     8701 atagatttac tgcatttgaa gtagaggatt caagtaaaac tatagagata tgcagagaat
     8761 ttgaaaataa atttaataat gagtcaggga tgcaaccata tttgtttttc tctaaagaat
     8821 taatgatagg aactgggaaa ttaatatatt ctacatttat atttttagca atatttatag
     8881 gtttaatttt ctttgttact acaagtagtt ttttatataa taaattttat atggattgcc
     8941 aagttgataa gaaaaagtat gagcaattaa ataaattggg gatgacatat aatgagatta
     9001 aaaaagcttc aacaattgag attggaatag tatttttgtt tccttatgta gtggctgtta
     9061 tacattcaat atttgccctt actgcactga aaaattcttt tgatatagaa gtagcatcat
     9121 cagctgtttt ggtaatggga agcttgttct tggttcaaat agtttatttt ttaattgtaa
     9181 gaaataatta tttaaaagag ataaggttaa atttagtcaa tttttagtta ctattattaa
     9241 atgataataa aaagggttta aaattaatta atattactgg aattatagat gtttattttt
     9301 tcagtaatat tttttattgt attaactatt aaaattttgt acaaatataa aatataatat
     9361 ggtaaatgtc aaaattataa aaaaattcta cttatataaa atataaagag gttatatgta
     9421 catatgtgta gaaataaata ataaaaatat caattaatgt tattaggggg ggttactcat
     9481 tagtaaatta gctaatacac ttagaatgat gatattaatt caaagcagag gcaaaatgaa
     9541 atgcaaagaa ttatcagaag agttggaagt aagtgaaagg caaataaaaa gttacaagat
     9601 gtatttagag caagctggta tatttataaa ttcaacacct gggatttatg gtggttatga
     9661 aatagataaa tgtaatagca tatcattaat taaattatta gatagtgaag tttcaattct
     9721 agatatgata aattcacagt tagagtataa taatgatatt tacaaaaatg aatttaataa
     9781 tattgtagaa aaaataaaag cagtattaaa tacaggagaa aaaagcgata catatatgga
     9841 ttattttaca gttcaagcac aacgtaattg tgattataag tctgaaaaaa ataagtgtaa
     9901 tgaaataatt agagcatata ccacaaaaca caaattttgg atagaatatt attcattaaa
     9961 ttcaggaaat agtgaaagaa ttgttcatcc atatggttta tttaattata aatcagatac
    10021 ttatatggta gctttttgtg aaaaaagatt taagtttata gactttaaat tatgtagaat
    10081 taaagattat aaggttttag aagaaaaata taatgtagat aaaagtttta gttgggatga
    10141 atatagtaaa aatagtatag gaatttataa aggagaagaa attaatgttg ttataaaaat
    10201 tagtcatcca ttttcaataa ttataaagga aaaagtttgg gttaataatc aacaaatcat
    10261 agaatatgat gataaatcta taatgtttaa agctaaaatg agaggttatg aggagataaa
    10321 aagttggatt ttaagtatgg gagcttatgt tgaggttgta gaaccagata gacttagaaa
    10381 tgatatattg tcagagattg aaaaaatgaa aaaaatttac tgattttttc aaataattta
    10441 tacttggacc aatatacttc acggtctagt gttaatatat taattgagag aggtgatatt
    10501 atgaaaatga aaattgaatt tagtacaggt tgtataccaa tatcttataa ttcgctcttt
    10561 atgagtatta taaaagaagc aataagaaaa tctaatgaag attactataa aaacatgtac
    10621 tattataaag aaaagaataa taaaaaaact aaaaatttta cattttcggt ttatataaaa
    10681 aagtatagta ttgaaggtga caattttatt atagaagata aagtaatatt aaatataagt
    10741 actccagatt tagaattggg ttttcatata tacaatgggt taatgacttc taaaaaatgt
    10801 ttatataaag cctatgaact tacaagaatt agaatcgatt tatcaagaga aaagaaggta
    10861 acagaagaaa gggtactatt taatgcattg tctccaatct gtgtaaagtc taaagaagga
    10921 aaattcttgg agataactga tgatagatac atagaagaac taaactatat aactaatgaa
    10981 gtggttaaaa attatagggg aaatggatta aaaagaaagt tagaatttga aaatataggt
    11041 ctcaaaaaga tagtagttaa agagagctta agagaattta aaaaaataac aggtaaagag
    11101 tatcaatata ttaatggtta caaaggtaaa tttatattga aaggtgatat tgatgattta
    11161 aatcttatct acaatctagg aattggtttt agacgtgctc agggatttgg agatgtagat
    11221 attttagaat ggaggtgaga taaatgaagt taagagttta tagaggagat tggtttttta
    11281 atatgggtat agttggattt ttaaatataa taaagaaagc agataaacaa gctgaaatat
    11341 ttattatgga agattatatc gaatttgata gcttatttct tgaaaacttt catgagtatt
    11401 attttaatta ctttatggat gaatatgatg tatcaaaaag aattaaaaaa aatattgatt
    11461 acagtataaa ttttattaaa tctaagcctg atagaatcaa agatggaatt aagaaaatta
    11521 aagatagtgt gaaacaacaa aatgataaga ttaaaaaatt tgatgaagaa aaatttaacc
    11581 tgataaaaga gaagctagat agcatgtcta agataaaaag ttatgatgaa tttgacagtt
    11641 tagaagcttt agtagatgag actatagata tatttaaaat aaaatcaata aatgacaggc
    11701 ttacagcaaa tctttataaa tatgtagttc aagataatta ttttggacaa gttagttttt
    11761 ttaatgttgc taaggcgaaa ttagatttag atggattaaa acagataatg tttaatgatt
    11821 atttaagaca aattatatat tttggagagt tagcagattt attagaagag aatgactatg
    11881 ataagttgaa aaactattta aatgatagat taaatagtat agctaaggat ataaatgaaa
    11941 aaaaagttag taagtctagt ataaatacta tagaaaaaat tatgaaggaa ataaataaaa
    12001 actttataaa gaaaaataaa agcatagaag atataaaaga gtatatggat agtttagaag
    12061 tatgtgaaat gtgtggttta tataagggaa tattagatga atactcagaa agtaattttg
    12121 ctccacttgg agtaagcacc aataatgcta ggaatatgtt ttggaatcag gcgtatgtac
    12181 catcaatatg tgatatttgt aagctaatat tgttctgtac tccagcaggg gctacttata
    12241 caagaaagaa ttatttaatt aatgaagaaa atgagtttta tttatttgtt aacatggata
    12301 cttctataaa tgagcttttt gaaagaaata atagtttaaa agctcaaaaa agtgaatatt
    12361 cagattccaa agatgaaaat ccttttaatc agcttataaa aagtatagta gaagaaaata
    12421 cattaaaaag tgaatggcaa cttcgtaata tactttttgt ggaatttaaa gcaagtatag
    12481 attctaaaaa atgtaaaata aattatttta atattccaac ttatttagca aaatttttta
    12541 cagacaagta tgcaaataaa aaaatacaaa gtatctatga ttacaagctt aaatcaaatg
    12601 tactagattt actcttgaaa aatagggact tgaagcatct aactaataac attttaagaa
    12661 ataaagttaa aaatgatatg gaaagcaaca acaaatcaaa tatatcagga atagattgtt
    12721 ttagagtagt ccaattaaga gcattaataa atagctacaa aaagggggta tataaaatgg
    12781 atagtaaaaa tttggagaag aatgatgaaa agctgagaat aatttattat ttaggttgtg
    12841 atatacatga ctattttgtt aataaaaatt ctaaaaataa aatagatgga gtaagctata
    12901 aacttttaaa ttcagttaaa gtcggaaata aaagtgattt tatggataca attataagag
    12961 tttttatgtc agctgaaaaa cagattccag catttatttt agatatagaa attgaaaaag
    13021 atttagactt tgaatctata ggtcatgctt ttatatctgg attaatttct ggaaaatatg
    13081 aaggaaaaga caaactacca aacgaaaagg aggaaaaata agatgaaaag agggttaaca
    13141 tttacagtaa tagcacaggc tcaaagttta aactatgggg aagggatagg taatatatca
    13201 gaattaaaaa aattaagtag agatgatgga aatatatata catttgcatc tcgccagtgt
    13261 ttaagatatg acatagtacg attagcagca gaattgttta attggaattt acaggttgtc
    13321 gataaagaaa aaggtactat tcaatttaaa gacaatataa gtataagaga atcagaagaa
    13381 atggatttat ttggatatat gaaaacaaat aaaaaagatg agaaagataa aaaaggaggt
    13441 tctttgacta gagaagctac tgtaagatta agtaatgcaa tttcccttga gccatataga
    13501 agtgacatgg actttttaaa taataaagga ttagcagata gaataggaga acatcctaat
    13561 cttgctaaca tagagcaaca tttaagttat tatacatata cagtgactat agatttatct
    13621 aaaataggta aggatggaga tatagaactt gataataaag agaaatgtag aagagtagta
    13681 gaatttttag aaataataaa agtattgaat agaaatatta gaggtagaca agaaaattta
    13741 tctcctctat ttgtagtagg aggagtatat gaaatagcaa atccattttt cttgggtaga
    13801 attaaattaa aaggagataa aaatggattt aaaataaata agcaagctat agaagaagta
    13861 attcaaggta cttttttagg aaaagattta aaagaattta cctatgttgg aatggtagat
    13921 ggggtattta taaataaaga agaatttaag ggattgtttg aagataattt tttaagtgta
    13981 gataaatttt ttggacaact agttaaagaa gtaaaggaat attatggggt gaattaatat
    14041 gaaggttttg aaattagatt tatttcaaga aactgcatgc tataaaaaac catttgctat
    14101 gaagattaca gagacatatc cactgcctcc atattctaca ataaatggat taattcacaa
    14161 aatattaaat gctacagaat atataccatt taatataagt gtacagggaa catatgaaag
    14221 tatatttaat aattaccaga caacatattt ttataaaaaa gatagtatta catctatgcc
    14281 aatgaatagt catatgctat tgaatgtaaa tttgataatt catataggtg gggattttga
    14341 tttattggaa gaaatatatg atagtttatt aaattatgac gagcacttat cattaggtag
    14401 aaaagaagat ttagtaagaa ttaatgatat tagatttgta gaagtcaata aatttgaagt
    14461 agatgaggat atacaattag atgattatga aaattataag ttatcagaat gtaatataag
    14521 aatgcctata tatataccaa aaacaagttt acaagataca gatatagatg gtataagtta
    14581 cagactcaat aattactatg ataataatgc tgtggaagat aaaaaaaggg tttggaataa
    14641 ggtagatagt tattatgttg aggatggaaa tactatatca attggagata tattattaga
    14701 taatgaagga gatttattat attttaataa tataaattag gagggagagt atgttatatg
    14761 ctaaatctaa tccagtggaa acacttagag aacatactga tgaactttta aaacaaatga
    14821 atgtattaag agaaagttat ggaaaaaata taaatagctt agattttcta gaggaagaga
    14881 tattttggca attacttgac tttgttatag aatttcatga tattggaaaa gcattttcac
    14941 cgtttcaaga gttaataaaa agcagaatgg atactaaaat aaatgaacct aaaatagtaa
    15001 ctcatttaga aaataatgta ggccataatt atctttctcc tgcatttatt gattatagtt
    15061 atatagacag gaaaaaaaat aaagaactta gagcagtatt aaatcaagtt attgtatatc
    15121 atcatgaaag agatatcttt atagataaag attttaaaaa tttaatacaa aaaatattag
    15181 atgaagattt aataaataaa gtttatgaac tacagcgtga atttaaagtt agatatccaa
    15241 taaaaacaga aaaattgagt aaagtatatc tacaaagtgt agaaaaaaga atagataaaa
    15301 atcacaagta ctataattta tatattatgt taaaaggaat actacataga ctagaccata
    15361 gtgcttctgc tcatgaagtt gtggaatgca ataatgttat aaatatagga gaacagacag
    15421 aaaattattt aatgaaagag tttggtagtt tgagagaggc tcaatcattt gctaaatcta
    15481 atagaaataa gaatataata ctgattgcat ctacaggaat gggaaaaaca gaaactgctt
    15541 tgatttggat agataaggat aaagcatttt ttacactacc attaagagta agtataaatg
    15601 cattatttga tagggctaaa aatataattg gagtaggaga agcaaatgat acattcttag
    15661 gtctgttaca ttcaacagca atagattatt tagaagaaag taatcaggaa aattcaagtg
    15721 agattgtaga tttagcaaag ttattatctt gtaaactgac attttccaca atagaccaaa
    15781 tatttaaatt tccttttttg tatagaggct atgaaaaagt gtactcaaca ttagcatatt
    15841 caaaagtagt aatagatgag atacaagcat attcaccaga aatagcagct gtattggtaa
    15901 aaggtataga aatgatacat aagataggtg gcagatttat gataatgaca gctactatgc
    15961 caaccatata tatagatgaa ttaaaaaaaa gaggtgtaat gaatagtaac ttagctgact
    16021 tgacatgtaa cacagaaaaa ataagacatt gtgtgtctat tgtagaaaat tcaatagatg
    16081 aaaacttagg taaaataata caaagtggaa tgaataaaaa agttcttgta atagtaaata
    16141 cagttaaaag tgctgtagaa aaatatgagt taatagaaga aatagttaaa ccaaaaggaa
    16201 tagatgtcaa cttaaatctt ttacattcta tgtatataca agaagataga gcaaaattag
    16261 aaaaatatat aaaagaattt gcagatagtg atagtaatgg tatttggatt actacacaat
    16321 tagtggaagc atcccttgat attgattttg atgagcttca tacagaaaat tcaacacttg
    16381 acagtctatt tcaacgattt ggtagatgtt atagaagtcg tgagtatgaa gaaaatagcc
    16441 caaatatatt tatatataca gaaaaagcta caggaatagg tagtatttat gataaggata
    16501 ttgtagaaaa aggacttgag ctactgaaaa cttttataaa tggaaaagaa tgtgtgaaga
    16561 tgaaagaaaa atataaggta gaaatggtta agatactata ctctaaagaa agtctagaag
    16621 gtacagcttt tgaaaagaga tttaccagtg ctttaaatat tttagatact ataacgccat
    16681 atagtttagg ttctaaagat gcacaaaata tacttagaaa tattgaagga tatacagtaa
    16741 ttccagagga tatatataat aaaattgaag aaactttaat caaagattat gaagagttgg
    16801 gacaagagct tataaatgca tatagtaatg atgatagaca attgataaat aaaataaagt
    16861 ctaagagaaa aaaagcaaga agagagataa ttaaaaaaac tgtaaattta ccaatatata
    16921 aagctaaaca aaatgtaatt gacatacagg tgaaaggtct tgaagattta aaaatacttt
    16981 tatataaata tgatatacac gaaaataagc atacaaaaca gatatttggt aaaggtgtgc
    17041 ttataagtaa tgaaatagac caatttattt gatttttata ttaacaatgt ggctaaaatt
    17101 tactaaagaa ggtgattttg tgccaataga tttagaagta ttaaaagttc aaggcgtaaa
    17161 aataaattat tattatgtat gtaaaagaaa actatggcta ttttctaaag gaataactat
    17221 ggaacaaaat agtagtagag tattgtctgg aaagatagta catgaagatt catataaaag
    17281 aatgaaaaat aaagaaatgt tgattgatga tatcttgaag ctagatataa taaaggacga
    17341 gtatgtaaaa gaagtaaaaa taagttcaaa gatgaaagaa gcagatgaaa tgcagttata
    17401 ctattactta tactatttaa agaatagtat gggtatagag aaaaaaggaa ctataaatta
    17461 tgttaaggaa aagaaacaag aagaattgat attgacagaa gaaaaagaaa aaattattga
    17521 aaacacactt ataaatataa atgaaataaa tatgcagtta tctcctccca aaattgagaa
    17581 aaaacattat tgtacaaaat gtgcttatta tgaattttgt tttgtgaagg aggagtaaca
    17641 atgtataaag actattatat tataagtaat ggaaatataa aaaggaaaga aaatacgatt
    17701 tactattaca atttagaagg tgagaaaaaa atattaccag tagaacaagt tgatgatatt
    17761 catatatatg gagaagtaga tttaaacaca aaacttataa attacataag taaatatggg
    17821 gtgatgcttc atttctacaa ttactatgga tattattcag gtacatatta tccaaggaag
    17881 aaaaatgtat caggattcag tttagtatgt caatcagcct gctatttaga taaaaatgaa
    17941 agattatact tagcaaaatc ctttgtggaa agtgcatcat accatatttt aagaaatatg
    18001 agatattaca aagttgatga aagtttaatt gatactataa aggatgaact aaatgatttg
    18061 aatgatgtca aagaaatatc agaacttatg ggtaaagaag gcagaataag aaaaacatac
    18121 tataaatcat ttaatcaaat attaaaagat gattttgaat tttataaaag agaaaaaaga
    18181 cctcccaaag acccagtaaa tactcttatg tcttttggaa atagcataat gtatacgaat
    18241 gttttaagtg agatatataa gacacaactt gaccctacaa taagcttttt acatgaacca
    18301 tcaacaaaga gattttcttt aagtttagat attgcagaaa tttttaagcc aattatagtt
    18361 gacacaataa tttttagcct gattaataat aaaagaatta ataagaatga ttttgaatat
    18421 caagacaaaa tttgttattt aagtgaaaaa ggaaagaaga aatttttgca agaatttgaa
    18481 aataagatgc agacaactat aaaacataga aatctaaata gaaaagtttc atatagaatg
    18541 tttataaggt tagagtgtta taaattaatt aaacatttta taaatgatga aaggtacaaa
    18601 gttttaaagg tgtggtggta agtatgtttg ttattgttac ttatgatatt gttgaagcaa
    18661 ggtcgttaaa tagaattaga aggatactta gaaaatattt gacttggacg caaaattctg
    18721 tttttgaagg caatattact gatggaaagt tacataaatg tatttctgaa atagaaaata
    18781 ttattgataa tagcgaggat tcaatctatg tttatgagat aaaaaatcct aattcaatta
    18841 aaaagaaatg ttatgggatt gataagtatt ctgatgaaat gtttatatag gtttgcagtg
    18901 agcgatattt atgctaaaat aggtgttaac agttggaata taagggattg aaggtgtatg
    18961 ataactgtta tcaattgcac tactgctcgc tcactgcaaa ttttgatgtt tttattgaat
    19021 tataattgct tgattgaagt attttcaatg tattcaatta tacctatttt ggggttttat
    19081 attaactaag tggtatgtaa ataagcaact atagtagaag caaaagcgac aacaactaaa
    19141 ggttttatat taactaagtg gtatgtaaat ttggttaact taagtgctat gaaaggaatt
    19201 aatgaagttt tatattaact aagtggtatg taaatttaaa ggtgcaagtc ttaatacttg
    19261 ttacatgagt tgttttatat taactaagtg gtatgtaaat aacaaagaac catttgaatc
    19321 acgaaaacta aaaaacagtt ttatattaac taagtggtat gtaaatttat taaccttatc
    19381 agaaacctga ccatcccaat tatagtttta tattaactaa gtggtatgta aattccattt
    19441 gtctaatgct tctaactctt tttttatacg ttttatatta actaagtggt atgtaaattc
    19501 caaaatgggc ttggagttaa gagttgtata agaaacgttt tatattaact aagtggtatg
    19561 taaattaatt acacactccc ttttaacgaa catattttag attgttttat attaactaag
    19621 tggtatgtaa atagactcga catagaatcg gttactaaat ccgagggttt caggttttat
    19681 attaactaag tggtatgtaa atactaaacc tttttgttta cttttatttc tcattttacg
    19741 ttttatatta actaagtggt atgtaaatcc ggatttttct gcttcgactg ttgttattga
    19801 gacttgtttt atattaacta agtggtatgt aaatagtaat tccatgcaat cttttatatt
    19861 gctttttaac ctgttttata ttaactaagt ggtatgtaaa tagatataca aggtgaaagt
    19921 gttgaaattt caatcttgtt ttatattaac taagtggtat gtaaatattc ttagatgtta
    19981 taatttgtgt tgcatttaaa tcgttttata ttaactaagt ggtatgtaaa tacaatatta
    20041 ttgttcattt catttaataa ttctagtagt tttatattaa ctaagtggta tgtaaatttg
    20101 tttatacctt taatctttaa aattaaatat aaaagtttta tattaactaa gtggtatgta
    20161 aatcattgta tactaagctt gatatagtgc aaaatgaaac gttttatatt aactaagtgg
    20221 tatgtaaata ctaattgggt taaacaaatg gatataacta aattagtttt atattaacta
    20281 agtggtatgt aaatccaacc cttcgcacga atccgtgcga cgaaatataa agttttatat
    20341 taactaagtg gtatgtaaat tgtaaaaata ttgcttagca tattgatatc actaactgtt
    20401 ttatattaac taagtggtat gtaaatacaa gaggtttttc agcaatatat gtacttctat
    20461 aatgttttat attaactaag tggtatgtaa attcatcaat attaattttg ggaatatcaa
    20521 cattttttgc gttttatatt aactaagtgg tatgtaaatg cgataagaga ttcgagtctt
    20581 tttactgggc ttaatcgttt tatattaact aagtggtatg taaataggat aaagaaaaga
    20641 ctcacacaag acacagtgtc aggttttata tcaactaagt ggtagataag tagaatatta
    20701 gcaatatcaa cgagtattta gaaacttatt ttatattaat aaagcagtat ataaaattgt
    20761 agaataaaca atagcatata ctaaaacatc caccttatat taactaaatc atatctaaat
    20821 aaaatgcact tacctataaa cattataaaa tcaatacaaa aatgaggtga aacaaaattt
    20881 atgataaaga aattaaacaa taaagacata aataaaatca tggaaatatg ggaaaaaagt
    20941 acaatcaaag cacatgactt tataagtaaa gaatactggc aaaataacta caatactgtt
    21001 aaaaacgaat atatacctat atcagataca tttgtatatg atgatggaga tgaaataaaa
    21061 ggatttataa gcataataga taaaagcttt ataggagctt tatttataga acccaaatac
    21121 caaaatctag gtatcggagg taaactttta gattatgcaa ctaaaaaata taaaagtcta
    21181 agtttagcag tatataaaga taataaaaaa gcagttgtgt tttataataa aaaaggtttt
    21241 aatatagtaa aagaacaagt aaatgaagat tcaggattta aagagtacat aatggaatat
    21301 agtaaataat atgattacat tctattaaaa gaataattaa tttattttct atttgatttt
    21361 tataagagta atttttaaaa tagactatat gaagataaag atatttatta tagatgtaag
    21421 ttaatcagaa ttgatatagc tacaaaagag gaaaataaaa ttgtatggtg tatttcagat
    21481 ttaacaaatt tactatttaa agataataag tttgatataa tattaaagat actttcacct
    21541 ataaactata gggtatttga aagaattttg aataattatg gaatgctaat aaaggtttgt
    21601 taaaatttaa taattattag attatagaaa attgccatga aattagttca gcaagaacta
    21661 gtttcatagc aattttaatt cttataagtg tatttatttt tacagattta aaaaacttat
    21721 acagtatcta ataatgtaat cttattactt tagcctataa atatttaaat cttaaaatgt
    21781 tctctttgag ataagtttat ttcagagtca caatttccac aacagtaaga tgttgagaca
    21841 tctttaaatt tttcagtatt ctcatcaaag ttcatttttt tctttgcaat aattcctacc
    21901 ttagttaaat tagaaccaca attattacag ttttgaattt tacataggtt atatagttta
    21961 ttaagtgtaa tatcttctaa taattcatca caattacaac atctgataga agaaacttca
    22021 ctagacattt caataggttc agcttcttta gaagaaaatt tatatgttat gtcatttgat
    22081 actttataac ctgtatcaaa tatattagaa ttacaacttg gacataagta ctttggaaga
    22141 tatatatctt ttttttcaaa agtatcacta ctaatattac agtttaattc atctaaaatt
    22201 ggtctttcat ctaggttatt acctttattt aagcttagaa gataattata aattttttta
    22261 tcttcattct gttttgatgt atctaacaaa tgctccatta tattagtgta ttttttgttt
    22321 tttgaaggca tgtttaaatc cagataattg ttttcaatag caaagcatac tatattgaaa
    22381 tagttatatt taactgcaaa aaataataac atttcatcaa atactttatc tattattttg
    22441 tcagaaatac ttatccaaaa atctggtgac tctaagtaaa ctttcctact aagttcatct
    22501 aactcttcat ctatattatt tatattattt tcattaactt tcatatcatc acttcctttt
    22561 aaatatcttg tgtataaact atttgttact attttttata taaaataaaa tcttatataa
    22621 aaatatatca tattataatt tacaagtata gtctaaatag agctgttttt aagcaaagtt
    22681 ttataaatga ctattaaaat ataaagtaac agtataaagt ttacttataa attaaacgta
    22741 aacctaaata cttgtaatgg ttgacaaaaa aacgtcttta ttttatacta aaacaaagct
    22801 ttaatagagg ctttgtatat gtacatataa actaaatgaa ataagacata tgaagggaat
    22861 tataaaaatg aaaaatacta aagtaaatat tcaagattta actaaaatgt caatatgtgt
    22921 agcattattg tgtatatcat cttacatata tataccattg ccatttactc cagcaggagt
    22981 aacagcacaa acaataatga taaatttgat agctttgatt ttaacaccaa gacaagcatt
    23041 tagtacagtt ggtgtatata taatgatagg tttaattggg attccagtat ttggtggagg
    23101 tacatctggt attggaaagt tgttaagccc aacaggtggt ttttattttg ggtttttgtt
    23161 tgcagcacta attattagtt tattaaaagg acgaaataat gatattaaga gatatatact
    23221 aataacaatt tttgttggaa tgcctattat atattttatg ggaacagtat ttatgtgcta
    23281 ttttaatcaa atgacagtag aagcagcact ttttgcagca gtagtaccat ttttaattgg
    23341 agatacaata aagtcggtta ttgcatcttt tttaggcact aaactgaaca atgtactcag
    23401 aagaaatcta tgataaatct ataatataag gaagggtgta atgtatgaag gaagttttta
    23461 tattaggagg attgagaagt catataggtc taaaaaatgg aatttttcaa tttgttcaac
    23521 ctgaattact aggagcaagt gttttaaaag atttaattaa aaagtatgaa attgacaaaa
    23581 ttgatgagat tatatgtgga aatgcagttg ggacaggagg gaatatagcc agattaatga
    23641 ctttaacagc aggagtgtca aatgaagtac ctgcttttac tgtggatatg cagtgtgctt
    23701 ctgctatgat gagtatagac atagcttttt caaaggttaa atctggtcag tgtaatttaa
    23761 ttattgcagg tggttttgaa agtagttctc tacaaccaat gcgcacatat cataagaatg
    23821 ataagcgata taatacaaat aatccaaatt atacagttgc tcaattttct cctgatgata
    23881 atagtcaaaa tagtatgctc gaaggggcgg aaagagtagc agagttatat caaattgaga
    23941 gagcagactt ggatttttgg gtaaaggaaa gtcataaaag agcaaaagaa gcaagagaag
    24001 aaaaaatact tgaggatatt atatctccta taaataatag tacctatgat gaaggtatac
    24061 gtgataagat gagtcaaagg ttattagata ggatgccacc aattttagga aaagaaacta
    24121 ttacaaatgc agcaaattca tgtcttatta atgatggagc ttcatttatt ataatatgtt
    24181 ctaagaagta tttagaacat gtaaaaaaga agcctaaaac aaaaattatt aatacatgca
    24241 caataggaac agatgcaacc ttaagtccaa catcagcgat acaagcaatg gataaacttt
    24301 tagagataga aagccttaat tatttagatg tatctgctgt tgaatttaat gaagcatttg
    24361 cagttataga tgtgttgttt caaagaaagt atccagagtt aatagagaga tataatatat
    24421 ttggtggagc tttagcgtat ggacatccat atggtgcttc aggtgcaatt attacattac
    24481 atcttttaaa agcattagaa aaaactaagg gtagatatgg aatttgctct atagctgcag
    24541 ctggtggtct aggttcagct ttactgcttg aaagggtgta gattatgaac tattatgaac
    24601 ttttaagaat aaaaaaagag aggcataaaa ataaaaattt tttaattata gatggagaaa
    24661 aatatactta tgaaaatata ttaagttata gtgagcaact aggaaaacaa atttctttag
    24721 gtgaaaatat ctcaatttta atatattcta aaaatataat gtttcagttg attagttttt
    24781 ttgctattaa ttattcaaaa aatattccta ttatttgtca ttataatctt tctaaaaaag
    24841 tattagatga tattttatta aaaaataata tttcaatcat tatatctgat gaatctatgg
    24901 acgttattga tttatatgat gataacaata atgaagtagc aaaaaaagtt aatttttcta
    24961 tttttaatcc taaatttaat ctatatatgt atcaatataa gaaccttata aattatttag
    25021 ataaaagtat ttgtatggga gctttatctt caggttctac aagtgttcca aaggtattat
    25081 atagaacata tgagagttgg gctggatttt ttcctataca aaatggtatt tttaatatct
    25141 ctggggaatc aatcttgttt gttaatggaa cacttagttt tacagggaat ctaaattcta
    25201 taatgtcagt attatatgaa ggaggtagta ttgtaataag ttcaggtctt aattgtaaat
    25261 catggataag gacaataaaa caatataatg taactaacat ataccttata cctacaaaac
    25321 ttcaactttt ggttaaacac ttaaaagaac cagtactcaa agtagtaagt atttttacag
    25381 ggtctcaatt attatttgaa tatactgcaa gaaatctaaa aaaatatatg ccaaagtctg
    25441 aaattatttt atactatggg gcaagtgaat taaattatat tacatattta tgctatgatg
    25501 aactaattga aaaacctcta agtgtaggca gaccatttcc aggaatagat atatatatta
    25561 gaggaggaaa aatttttgtg aatacagaat atgcagtata taatgcaacc aaaccttatt
    25621 ctgtcaatga tattggatat tatgataatg atggatactt aatatttgaa ggaagaagtg
    25681 atgacattgt aaatattgga ggatttaaag ttagttctgc taagattgaa aatgaagtta
    25741 aaaaaattcc tcaaattgaa gatgcaattg tattgccgta ctcagacact ataagaggaa
    25801 atcaaattgt attatttgta ataactatag acaaaattac gaagaaagat ttgttgatta
    25861 aaatgaaaga taatctaatg aaaaatgaaa tcccaaagaa aattatcttt ttaaattcat
    25921 ttccatatac ctcttctgaa aagatagata gattagcatt attaaaaatg ttataagtat
    25981 aatatttagt aattttagtg aaccaatgta ttgataaaaa catattgtat atagtaaagg
    26041 aggcttacaa tatgtcaaat caatacgata taactgttat tgggggagac ctgcgacaag
    26101 tgtacatggt aaaaaaacta attaataaag ggtattctgt agtcacttat gggatatcaa
    26161 atgaaattat aaatggaatc gttggaaaat caacttcact agctgaagca ctatccaaaa
    26221 gttccattat tttatgtcca attccattta caaagaatca agttaatatt gaaaatacgg
    26281 atgattgtct tgatgctact attgataact tgctaattaa tttatctcct aatcaaatat
    26341 tatttggagg taacatacca actaaagttt gtaaattttg cgagcaacac tctattcaga
    26401 tttatgattt tatgaaaatg aatgatgttg ctattttaaa tgcagttgct acagctgaag
    26461 gggctatagc agaagctatt aaaagaagta taatcaatat acatcaaagt aactgtttag
    26521 tattaggtta tggaagatgt gcacaaatac ttgcaaaaaa attatacgct ttggatgcaa
    26581 atgtatgcgt tggtgcaaga aataatgatg caagaagtac ggctaatgca tttggatatt
    26641 ccagtatttc tttaagtcaa cttgatgaaa aacttttaca atttgattat atttttaaca
    26701 ccataccaga gtgtatttta acgaaagaaa gattagaaaa agtatcacaa gaagtaacta
    26761 ttatagatat agcttcttct cctggaggag tagattattc tgttgttaaa gaagtaggga
    26821 ttaatgcgac tctttgcctt gggcttcctg gtaaatattc accaaagaca tctggtgaga
    26881 ttcttgttaa cgctatagaa aatattataa atgaaagaag ggattagaaa tgagacttga
    26941 aggacttaca attggagtag gatttactgg ttcgttttgt acttatgata aaatatttat
    27001 agagttagag aatttggtta aagaaggagc taatgtacac actatatttt cagatgtatc
    27061 acaaaatatc gattgtagat ttggaaattc tgaagagttt atgaaaaaag catatgaact
    27121 tacaggaaat aaacctatag ttacgattga agaggcagaa ccatttggac ctaaagggat
    27181 tgctgatatt ataataatag ctccttgtac tggaaatact gctgcaaaat tagcaaatgg
    27241 tattacagat tcaccagtat taatggcagc aaaaggtcat cttcgtaatg ataaaccact
    27301 agtaatatca atatcaacga atgatgctct aagctttaat ttcaaaaata ttggtattct
    27361 ccttaattct aaaaatatat attttgtacc ttttggtcaa gataattgca aagcaaagcc
    27421 taattctatg attgcacata ctgagctaat aattccaact atagaacttg ctctagagaa
    27481 caaacagcta cagcctgtta taaaaagtcc acatcaatag aattggttct tataatatta
    27541 tgtattagat acaatactag caaagaggct gtctcaaaat agagagaagc ctctttttca
    27601 ttgttgaaat gatgaaaata tatttaattt catacaaaaa aatgcatcat gaactaaaaa
    27661 gtctattttg agctaatctc cgtaattttt ggtatattaa aattatttac ttaatccaat
    27721 ttttaaatta gaatttatat cgttttgact cttcaaatat caatctaact gttgttgata
    27781 caaaagaaga ggacataatt aaaacaaata ctgatagtcc tctaataaac aaattatcta
    27841 cataataagt tactattcca atacctccaa tcgttaaaat tacacttata atcaaagaaa
    27901 aaatgctaat tgtttttaat tttttcatgc ttatcctcct tcttataaac ttcaaattta
    27961 tctatataat attaaatata ttttatttca tagtatataa aaatcatgaa tatat
//
LOCUS       DAMJVX010000030        27970 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMJVX010000030 DAMJVX010000000
VERSION     DAMJVX010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27970
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.032"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            119..1492
                     /locus_tag="PC407_001278"
     CDS             119..1492
                     /locus_tag="PC407_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2496735.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILANSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIEKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDILLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEGKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1748..2815
                     /locus_tag="PC407_001279"
     CDS             1748..2815
                     /locus_tag="PC407_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HDF2496736.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGDVLIEMEHPQQIIPEVE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            2912..3220
                     /locus_tag="PC407_001280"
     CDS             2912..3220
                     /locus_tag="PC407_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HDF2496737.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKVNPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3213..4643
                     /locus_tag="PC407_001281"
     CDS             3213..4643
                     /locus_tag="PC407_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HDF2496738.1"
                     /translation="MNNSTSIKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGAKIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            4721..5095
                     /locus_tag="PC407_001282"
     CDS             4721..5095
                     /locus_tag="PC407_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HDF2496739.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWNLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5089..7293
                     /locus_tag="PC407_001283"
     CDS             5089..7293
                     /locus_tag="PC407_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2496740.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLRIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7293..8702
                     /locus_tag="PC407_001284"
     CDS             7293..8702
                     /locus_tag="PC407_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HDF2496741.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELYIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8687..9751
                     /locus_tag="PC407_001285"
     CDS             8687..9751
                     /locus_tag="PC407_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HDF2496742.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERSIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKKLQDINLPKILIRIPMLSELEEVINYCDISFNSELETI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            9781..11202
                     /gene="nhaC"
                     /locus_tag="PC407_001286"
     CDS             9781..11202
                     /gene="nhaC"
                     /locus_tag="PC407_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2496743.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11631..12095
                     /locus_tag="PC407_001287"
     CDS             11631..12095
                     /locus_tag="PC407_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2496744.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12393..12755
                     /locus_tag="PC407_001288"
     CDS             12393..12755
                     /locus_tag="PC407_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2496745.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            13350..14210
                     /locus_tag="PC407_001289"
     CDS             13350..14210
                     /locus_tag="PC407_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2496746.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIIANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            14468..16582
                     /locus_tag="PC407_001290"
     CDS             14468..16582
                     /locus_tag="PC407_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2496747.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEHDVELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYVGTLTQLISM"
     gene            complement(16844..19087)
                     /locus_tag="PC407_001291"
     CDS             complement(16844..19087)
                     /locus_tag="PC407_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2496748.1"
                     /translation="MSNIIIKGLTQNNLKNISFKIPKNKIVVFTGVSGSGKSSIVFDT
                     IAAESQRQMNETYSSFIRNRLPKYRKPQVELIDNLTASVIVDQTPLGGNARSTVGTIS
                     DLYSSLRLLFSRIGEPYIGTTSYFSFNDPNGMCKNCSGIGRVKVINIESIINPENSWN
                     TGCINDSLFKPNSWYWKQYAESGLFDLDKPIKDYSFEEYNLLLYGSKNKDGKQENPKI
                     EGIFNKYNQTYLNRDISNMSKHTKEKSERLITDKECPVCHGKRLNKETLNCNILGYSI
                     ADMCNMELTDLYDLLLQIKDKRTETVIKTLTDGLKRMIDIGLPYLHLNRESSSLSGGE
                     AQRLKLVRYMGSSLTGMTYIFDEPSAGMHPRDVHRMNQLLKKLRDRGNTVLVVEHDKD
                     VISIADEVIDIGPSAGTQGGHVVFQGSYDKLLLANTLTSNAMNLSLPIKTDSRHPKGT
                     LPIRDACINNLKHIDVDLPVGIMTVVTGVAGSGKSTLISRVFAQKYEKDVIKIDQSPV
                     TATNRSMPATFLGFFDEIRKVFAKENNVEEGLFSFNSKGACPVCDGKGVLVTELVFMD
                     PIINTCEACKGTRYSEEVLSKLYKGKCIVDVLSMTVDESLEFFTEKKLCKILQTMKDV
                     GLPYMTLGQPLSTLSGGERQRIKLAKNLNKKGTIYILDEPTTGLHGSDIEKLMLIFEH
                     IVDKGNTVIIIEHNLDVLKQADWVIDIGPDGGKNGGQVVFEGTPLEMFERGCTITSDF
                     LRKSINI"
     gene            19419..20135
                     /locus_tag="PC407_001292"
     CDS             19419..20135
                     /locus_tag="PC407_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496749.1"
                     /translation="MILIVILSIFIIASINIIILGKENIRDIVIPLTLFIVSDFLFLC
                     GNIICRGGVMLLFIGLFAIPLMLIAFIWICVHMIKLYYKKDLNKRKMIGCIFSIFISI
                     GTIFIPSPSESFRFKLYEKDYSVVAKAILKVYDEKKLSSESVFYIQSDKNELEKIFSK
                     EVIKKMKKLNRNLDVVKISGNRNLVYFFFGAELQSIDGIVIFKDLKHVKPKLDFINRS
                     IQAPRLEYITNNVYYFKGEL"
     gene            20163..21101
                     /gene="blaCDD"
                     /locus_tag="PC407_001293"
     CDS             20163..21101
                     /gene="blaCDD"
                     /locus_tag="PC407_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2496750.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            21445..22128
                     /locus_tag="PC407_001294"
     CDS             21445..22128
                     /locus_tag="PC407_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2496751.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            22125..24785
                     /locus_tag="PC407_001295"
     CDS             22125..24785
                     /locus_tag="PC407_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2496752.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAQKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDRTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKMS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKTVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNTRSRRQEFAMLRSVGLSPE
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(24966..26147)
                     /locus_tag="PC407_001296"
     CDS             complement(24966..26147)
                     /locus_tag="PC407_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2496753.1"
                     /translation="MKTPRNINSTVVFIIFCLIITFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(26147..26809)
                     /locus_tag="PC407_001297"
     CDS             complement(26147..26809)
                     /locus_tag="PC407_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2496754.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCGNISLNKVEVKAYLDNVEVLLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            complement(26954..27496)
                     /locus_tag="PC407_001298"
                     /pseudo
     CDS             complement(26954..27496)
                     /locus_tag="PC407_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(27614..27970)
                     /locus_tag="PC407_001299"
                     /pseudo
     CDS             complement(27614..27970)
                     /locus_tag="PC407_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT        10661 a         3374 c         4830 g         9105 t
ORIGIN      
        1 aaatatcata aatatatgat aagataaata caatagcaat attttattaa ttttttgggt
       61 ttttatatat ttaggtactg gggtgatata gttgaaccaa gagatatttt ttaaaataat
      121 gaatgaaata ttggaaaata ttgatgaagg ggtacatttt gttaataaag acgggaaaac
      181 tataatctac aataattctg tacaaaaaat ggaaaaaatg aacagacacg atattttagc
      241 caattcattt tttgatatag tcaacaaaat gaaaataaac aggagtacac tattagaagt
      301 tttggaaaaa agggaagtta tcaaagacaa tattcaaaaa tatctggaca aaaatggaaa
      361 agagataaca gcaattaata ctacaatacc aattgatatt aaaggcgagt ttgtaggggc
      421 acttgaaata tcaaagaata tgacaacgat agaaaattta ctaaacagaa attataaaaa
      481 aaatttagtc aataaagtta gtagtagaca aaaaaagggt tatgaatttg atgatatttt
      541 aggggaaagt aatttgatta aagaagcaat agaaaaatct aagaaagctt gcaagtctga
      601 tgcttcagta tttatatacg gtgaaactgg aacgggtaaa gagttattta gccagtccat
      661 tcattatgga agtaatagga gaaactatcc atttatagca caaaattgtg cggctttgcc
      721 ggaatcacta ttcgaaggta tcctttttgg aactagcaag ggaggattta caggagcaat
      781 agataggcca ggcttatttg aacaagctaa caaagggaca ttgttattgg atgagataaa
      841 ttccatgcct attatgttac aggctaaact attaagggtt ttacaagaag gttatgtaag
      901 aagggttgga ggaactaagg atatacctat agatgtaaga attattgcaa cgacaaatga
      961 aagtccaaag gtaattttaa acgagcataa aatgcgaaaa gatttatatt ataggcttaa
     1021 cataattcat ataaaaattc ctcctcttag agatagaaaa gaggacatac ttctattgtg
     1081 caaaaaattt atttcaaagt ataataaaaa gttaaataaa aaagtggtag gaatttcaga
     1141 tgaagctatt aaaattttaa aaaattacga ttggcaaggt aacataagag agctagaaaa
     1201 cataatttat tgtactatga gtatgatgga agatgaggta caaatagaaa caagtatgat
     1261 taatctaaac gactattaca atcttaaaga gaataaagag agtaacaatt attctttaaa
     1321 cgatttggag ggcaaaacac ttagtaagat tatcagtgag atagaagaaa agtatatata
     1381 tgaatcatta gaaaaggcat cgtacaatat aactaaagct gcatcaattt taggtatgaa
     1441 taggcaaaat ttacagtata aaataaaaaa atataatatc aaaatattat aaataagcaa
     1501 gataaaaaaa caaaatagaa gtaaaataaa aaagcaaaat ataggagcaa aattattata
     1561 aaagagcaaa atattttgct ctttttttta tttctcataa gaaatattat tttttattta
     1621 tgaaaataca taataaaata attatagttt caacgttcca ataaatattt actttaattt
     1681 taaagttggc acgatttatg ctttaactaa ttattgagaa tatataaatt tttaggaggg
     1741 tgtatatatg agaaaagtaa gagtaggaat atggggattt ggagctatgg gaataggtat
     1801 ggctaatatg attctaaaga aagaaggtat agaaatagta tctgtttgta gtagaagtac
     1861 ttcagggaaa agtatgtatg atgttttagg aattgaaaga ggagaaagac cagaagttat
     1921 tatcaataaa aattatgaag aagtttttag agaaaaaagt gtagatgttg ttttgttagc
     1981 aactgattca tttactaaaa aggcttttga taaaataatt ttcttattaa atagaaaaat
     2041 aaatgttata tcaacagcag aacaaatggc atatccacaa gctgatgatg cagatttagc
     2101 taaaaaaatg gatgaagttg caaaggaaaa tggagtaagt atacttggaa caggaataaa
     2161 tccaggattt gtattagatt tactagtatt ggcactttca ggtacttgtg aagaagttac
     2221 tagtataaaa gctaagagag taaatgattt atctccattt ggaaaatctg ttatggagga
     2281 acaaggtgtt ggagtaacaa gggaagaatt tataaaaggt gtagaagata agactatagc
     2341 aggtcatgta ggttttgtag aatcaataaa tatgatagct gatggattag gttggaaatt
     2401 agataagata gaacaaacaa aagagccaat aatgactaca gtagatagaa aatctaaata
     2461 cggagaggca ttggctggaa atgttgctgg ttgtagacaa tgtggatatg gatatgtaaa
     2521 tggagacgtt ttaattgaaa tggagcatcc acaacagata ataccagaag tagaaggtat
     2581 taaaacagga gactatgtat caataaaagg tataccaaac attgatttac aaataaatcc
     2641 tgaaatacct ggaggagtag gtacttatgc aatgatagta aactctattc cattaataat
     2701 aaatgctaga ccaggtctta aaactatgtt ggatatacca gtgcctagag caataatggg
     2761 agacataaga aatcagatag aagttgaatt ggaagaagaa tcaaaggcta attagtaaga
     2821 taacagttag ctaattggta aaataatgtt taatttaaaa taacagtaaa ttaattagta
     2881 aggaatagtc aattaataag gggatgaaaa aatggatgct aaaattaatg attgggttat
     2941 tatacacaat atagtattga ctccagaaga aagagctcca caagttccag aggatactaa
     3001 gaaagtatcc ttagaaatgt gggttaaagg ttttatacaa agtgatgctt ctataggcga
     3061 cttagtagaa gttaaaacaa ttacaggtag acttgtaaaa ggtaatttac ttaaagtaaa
     3121 tccatattat actcatgatt atggtaaatg tataccagag ttactccaaa taggaatcca
     3181 agctaaagaa atattatttg gaggtgtata taatgaataa ttcaacatca ataaaagata
     3241 tgagttatca agctgttatg ggaagaaata acgaaataat gaaaaatgca atcggattgg
     3301 attattcttc ttttgaacaa gagggaatag ggtttgatta tgagaaaatg atgagtgaaa
     3361 caggatatac attacaagat attgaagcaa ttcaatcaca gtatgctgtt ggaaatactc
     3421 ctctaataga gcttaaaaat ttgactaaat tagctagaaa atgtgctaaa gaaggtaaag
     3481 gagctaaaat atttgtaaaa gatgaagcaa tgaatgcatc tggtagtttt aaagcaagaa
     3541 gagcagctac tgcagtatat catgccaaac aaatgggata taaaggtgta atagcagcta
     3601 ccagtggtaa ctatggtgca gctgtagcat cacaagcggc aatgcaggga ttaaaatgta
     3661 taatagttca agaatgttat gattctaatg gtgtaggtca acctgaaatt atagaaaaag
     3721 cacgaaaatg tgaggcatat ggagcagaag ttgttcagtt aactgttgga ccagagttat
     3781 tttatacgtt cttagtttta ttagaagaaa caggctattt taacgcatca ttatattcac
     3841 catttggaat tgcaggtgta gaaactttag gttatgagtt agctatccaa tttagagaaa
     3901 agtataaaaa agaccctgat atagttgttt gtacaaatgc aggtggtggt aatcttactg
     3961 gaactgcaag aggattgata aaagctggtt ccattaatac taaagtagta ggagcaagtg
     4021 tagatttaaa aggattacac atggctagtg ataatcaatt taataaaaaa tcatttacaa
     4081 caggacatac aggttttggg ataccatact gtacatggcc tgataggtca gatgtaccta
     4141 ggtctgcagc tagaccactt agatatatgg atagatatgt attagtaaaa caaggagaag
     4201 ttttttatac aactgaatta ttggcacagc tagaaggtat agaaagaggt ccagcaggaa
     4261 atacatcttt agcagcagca ttctctttag ctcaagagtt agatgaggat aaagttatag
     4321 tggtacaaga aacagaatat acaggcgcag gtaaacatat atgtccacag cttacttttg
     4381 caagagaaaa tggaatagat ataaaatttg gaaatccaag agaagaaatt gcaggagtaa
     4441 acttaatatt accagaaaga ccagaattat tgaaatgtgt tgatattgat atgaataaaa
     4501 taagaaagtc atttataaaa aattgcatat ctaataatca cattgataat attgataagc
     4561 taagtaatcg agatatagaa tttttaatgg aagaagtaaa aagttcaaga gattttgttg
     4621 ttgatgtatt aaataattta tagtaattat tgttttttta tttaatataa aataaaaatt
     4681 cggaaaattc tattaaatca atgcgaatgg aggaatgttc atgaaaaaaa gagaggatga
     4741 ttttgaagtt agaagaaaac atcttcaaga actgagtgat gatgagttaa aagaaaggtt
     4801 ttggaattta gctacccaga tagtagaacc aatgatagaa cttggtaaaa agaatacaac
     4861 accatcaata gaaaggtctg tcttgttgag aatgggattt tcttctcttg aagtaaaacc
     4921 tattttagaa ggggttatgg aaagaggtct tataggtaaa ggagcagggc atgtagttta
     4981 taaattggct aaatctaaaa atataacggt tagagaagca ggtttgttat tagttaaggg
     5041 tgaatattgg gatgaagtta catgtttatt tagagaaggg gtagaatcat gttaaaggaa
     5101 aataaaaaat tggatataga ctatatatta aaagatttgg ataaatataa accaaaacga
     5161 agaggttggg tttggagaga acacttggaa aatcttgaaa tgggaccttt taaatataaa
     5221 gactgtacaa aacctttaaa gaagagtgta gggctaccat catctaagta tttcaataat
     5281 atagaccctc aaccaagtcc agttattact actgaaatag cttctggtag atttgaggat
     5341 gatataagaa gaatgagaat ggcagctcat catggagctg accatctaat ggttatcaga
     5401 actgctggac aatcacattt tgatgggctt atagaaggaa ctccacaagg tataggtgga
     5461 gttccaataa caagaaaaca agtaagagct caaaggaagg ctcttgattt tatagaagaa
     5521 gaagttggta gacctattaa ttaccattca tatgtaagtg gtgttgctgg tcctgaagta
     5581 gcagtaatgt ttgctgagga aggagtcaat ggagcacatc aagacccaca atataatgtt
     5641 ctgtatagga atataaacat ggtaaggtct tttgtagatg cttgtgaggc aaagaaaata
     5701 atggcttttg ccaatatagc tcaaattgat ggagcacata atgctaatgc tactgctaga
     5761 gaagcatgga aggtaatgcc tgaacttatg gttcaacacg ctttaaactc tatattctca
     5821 gaaaaaatag gtatagataa aagtaatata tgcttatcaa cagttccacc aacagcacct
     5881 ccagcaccat gtttaagaat agacttacct tacgctgttg cacttagaga attatttagt
     5941 gattataaga tgagagctca aatgaatact aaatatatgg aatcatcaac tagagaagca
     6001 acagttactc atgtattaaa cttattaaca tcagtattaa ctagagctga catacaatca
     6061 actataacac ctgatgaagg tagaaatgta ccttggcata tatacaatat agaagcatgt
     6121 gatacagcaa agcaagcttt agttggtatg gatgggctta tggatatgat agaacttaaa
     6181 gatgtcggtg aattaagaga caaagcaaga gaacttaaag aaagagctgt attatatatg
     6241 gaagaaatat ctgaagttgg tggatatttt gaatctgtgg aacaaggatt ttttgttgat
     6301 tctggaaatt atccagaaag aaatggtgat ggaatatcta gaaaaataaa aggtggagtt
     6361 ggtgaaggta ctgtttatga aagagaagaa gattatttag cacctgtaac tgctcacttt
     6421 ggatacaata atgtagctca atatgatgaa aatgctatag ataatccatc aatgttaata
     6481 gatggatgta catttgaaaa tccagacaaa ataatatata ttgatgagct tgacgatttt
     6541 gataatgtag aaaatagact aaaagaatca tatgagtaca gaaatggaac taaaattaag
     6601 cctgaaatgg aatggtgtgc agatggaatt gtaatgataa caatgatgct tcctactgat
     6661 aaaagaacag cagagtttgc agctttggaa tttgtcaaaa aaatgaactt acaagaaatt
     6721 gaggtcataa gtagagaagt tatgcatgaa tctgaaggga ctagaataga agtgaaagga
     6781 agagttccgt ttgatataga tttaaacaat ttagtaatac cagaagaacc taaggtacta
     6841 actgatgaag aaataagagc agatatagaa gaaaaaccta tgaaaatagt ttcagctacg
     6901 gttggagaag atgaacattc agttggttta agagaaataa tagatataaa acatggaggt
     6961 attgaaaaat atggcataga atgccactat ctagggactt ctgtacctgt agaaaaattg
     7021 gtagatgcag cgattgaact gaatgcagat gcaatacttg catctacaat aataagtcat
     7081 gatgatattc actataaaaa catgaaaaaa ttacacgatt attgtgtaga aaaaggtata
     7141 agagataaag taatgattgc gtgtggagga acacaagtaa cacctgaaat agctgttgaa
     7201 caaggcatcg atgctggatt tggaagaaac agcaaaggca tacatgtagc cactttctta
     7261 gttgaaaaaa gaagagaaat gagtaataaa taatggaaaa tagcaaagtt tctttaaaaa
     7321 tagatgtgtt ggtagctgaa attggaagta ctactacagt ggtaaacgct ttccacgata
     7381 ttaatactaa caatcctata tttttgggtc aaggtcaagc tccaacaaca gtgtttgaag
     7441 gtggcgatgt aagaaatggc ttatcaggtg ctattaaaga tttagctaat aaattatctg
     7501 tagatgatat agagtataat gatatgtttg caacttctag tgcagctggt ggattaaaaa
     7561 tgactgttca tggattagtc tatgatatga cagtaaaagc agctaaagaa gcagctttag
     7621 gggctggagc tgttattcgt caaataacat caggtagaat taaaagaact gatttaaata
     7681 aaataaaaga aataaaccca aacataattt taatagctgg tggagtcgat tatggagaaa
     7741 gagatacagc tatttataat gcagaaatga tagcttctat gaatctaggc ataccagtta
     7801 tatatgctgg taatgttgaa aatcaagagg aaataaggtt aatatttgaa gatactaatt
     7861 ataagcttta tataactgaa aatgtatacc ctaaaataga tttattgaat atagaaccta
     7921 caagaagaat cattcaaagt gtatttgaag aacacataac tacagcacca ggaatgaaat
     7981 acattaaaga aatggttaat gaaaatatta ctccaacccc aggagctgta atggaagctt
     8041 ctaaactttt atataaaaat attggagatt tattaacttt ggatgtaggt ggagcaacta
     8101 ctgatgtaca ttctgttaca gatggaagtg actatattaa taaaattttg gttaatcctg
     8161 aacctactgc aaagagaagt gttgaggggg atttaggtgt atatgtaaat atgaagaata
     8221 tagtagaagt tataggtaaa gaaaacttac aaagtgaact atatatagat attgatgcag
     8281 taatagagaa ttatcctcca ataccaaaat caaaagaaga aatattattt gttgagaggt
     8341 tgacaaaaga agctgttgtt aaagctatgc taagacattc aggaaaaata agaaatatct
     8401 atggaacaac tggaaaagtc aaaattgcag aagggaaaga tttaacagaa gtaagatata
     8461 tagttggtac agggggagca cttactaggc tccctagtag aattgaaata ttagataata
     8521 tgctcaaata caataaaaat aatgaattac tatttccaaa ggaaaaaaca aagatattaa
     8581 tagataatga ctatattatg gcatctatgg gtgttttatc taaaaaatat gaagaagcat
     8641 ctcttaagct cctgttaaaa agtttgaatt ttgaggagga aagattatgt atcctaggtt
     8701 agaaatagat atagaaaagt taaagcataa tgcaaaatta atttcgcaga tgtgtcatga
     8761 aagaagtatt aagatatctt ttgttactaa atcattttgt gcacaaaaag aaatagtaga
     8821 agaaatatgt agtgaaggaa tagaccatat agcagattca agaatacaaa acttaaaaaa
     8881 attacaagat ataaatctac ctaagatatt gattagaatc ccaatgttaa gtgaattaga
     8941 agaagttatc aattattgcg atattagttt taactcagaa ttagaaacaa taaagaaatt
     9001 aaatgaatta tgtgaaagta aaaatattat tcataagata gttcttatgt ttgacttagg
     9061 cgatttacga gaaggatact tttatgaaga agactttttt aataatgtaa gagagatagt
     9121 aagattaaaa aatattgaaa taattggtat agctacaaac ttaacctgtt atggagctat
     9181 tataccatct agggaaaata taggcagact ggtaagtata gcaaaaagaa tggaagaaaa
     9241 atttggaata aatctagaaa tagtatctgg aggtaattca agttctattc atctgttgat
     9301 aaataataat atgccagaag gtattactaa tttgagaata ggcgagtcca ttctacttgg
     9361 tagagaaacg gcatacggtg aaaatataaa tggaacatat caagatgcat ttaaattaat
     9421 atgccaagta gttgaatgta aagaaaaacc atcagttcct attggagaaa taggagttga
     9481 tgcctttaga aataaacctg tttatgaaga taagggaatc ttaaaaaggg caatcatagc
     9541 tataggtaaa caagatataa atattgactc tttaattcca atagacactg atataaaaat
     9601 attaggggca agttcagacc acatgatact agatgtaagt aacacaaaat atgattataa
     9661 attaggtgac aatttagaat ttttattaac ttatgggggt atcatgtcat ctagtacaag
     9721 caaatatgtt tcaaaaaaaa ttatagtgta gtgtttaaat aattaggagg aaactatttt
     9781 atgaaagagc gagaatgtag aaaagcaagt ctgtttgatg caatgatacc aattacttgt
     9841 ttgatattat ttttaagtct aggagtatta gtgtatggtt catcacctca tgtaccacta
     9901 ataggagcag cagcagtagc aggacttgtg gctgtatata gacttggatt taaatggaaa
     9961 gagttagaat taagtatgtt taatagtata aagatggcaa tgcaagcaat cttaatcata
    10021 attataatag gcgtacttat aggaacttgg atagtaagtg gagtagttcc atgtatgatt
    10081 tactggggac taaaaatttt atcaccaaat atttttttag tagcatcaac attagtctgt
    10141 gcaatagtat ctcttgcgac aggttcatct tggagcacaa tgggtacagt aggagtagct
    10201 cttttaggaa ttggtcaaag tttaggtatg ccaattggat taatcgtagg ttctataata
    10261 tctggagctt actttggaga taaactatca cctttatctg aaacgactaa cttagctcca
    10321 gcaatggcag gaacagattt gtttacacat ataaaatata tgttatattc gactatacca
    10381 tcattattaa tttgtttagt aatttatggt gtaattggaa tgaagtattc aggtcaagca
    10441 ctagatataa aacaaataga attaattagg tctacattag attctacatt taatacttta
    10501 tcaccagtat tattattagc tccagcaata gtaattggat tagttgtatt taaagttcct
    10561 gctataccag ggcttatagt aggtgctgta ttgggagtat tgtttgcagt agtttttcaa
    10621 ggagaatcta tgaatacgat attagatgca gcacaaaatg gatatgtttc gtcaacacaa
    10681 atacaggagg tagatgcttt attatctaaa ggtgggatta tgaatatgat gtccactgta
    10741 tccttaacaa tatgcgcttt atctcttgga ggtattttag aaaaaacagg tatgcttgaa
    10801 gtagtagcat cttctttact aagattagca aagggtgtat ttggaacagt attatgtact
    10861 atggtaactt gtacaattac caatataata gcaggtgaac aatatctatc tatagttata
    10921 ccaggaagaa tgtataataa ggaatataaa aagagaggta tacatcctaa gatgttgtcg
    10981 agagctttag aagatagcgg aactttaact tctccattag taccatggaa tacatgcggg
    11041 gcgtatataa cagcaacatt aggtgtatct gcactaacat atggtccata cgctttatta
    11101 aatattataa atccaatagt atctttagta ttaattgcat gtaagtttaa aatagcaaga
    11161 atagaagatg aacctgaaac ttgtttaaat tttgatgttt gataatagat aacttatgta
    11221 agaacgtagg aactaggggg ggagcctgtg gggaattagt aagacatatt ttagtgtgcg
    11281 aaattctata aatcagtaat cttgtgactt tagccatggg aggttcagat tagtaaattt
    11341 atagttttat attagtctat actttgatct tttaattgtt tgtataaatt ttttatagaa
    11401 aaagatttct tttagacttg cctaaaaatt aggcaagtct tttaagtatg ttctgattat
    11461 aagagcactg tgtgacaaga atgatgagat tatttttgct atactttcga tataagaaat
    11521 ggaaggaggt tatgttttat atttatatga aataattagt tacacagtga attttcgtat
    11581 aatttggtat aaaaagatta gaaatgaaaa taaagtaatg gaggaataaa atgactagaa
    11641 tatccaatat aacaatagct gaacaatctg agtactgttt tttggcaata cgaaaaacaa
    11701 ttgactttat ggtagagttt tcagagtttt caaaacaaag ttttgaaaaa atttctaaat
    11761 atctggagga gagaggaatt ttatcttcag gagcaccgat tgtatgtttt cataacatgg
    11821 atttagcaaa attggatgtt gaagtagggt ttcctgtagc cacttatata gatgggaaaa
    11881 atgagattct acaaaggatt gttcctgcac agaaaataat aactgcaatt gatttaggac
    11941 cttatgaatt acaagaccct actttagaag acttattttc atgggcaaat agcaatggat
    12001 ataaacttca tggcgatata tattaccagt atttaaatga tatcaatcgt ccagctagtg
    12061 aatatcttac gaaaatgatg ttacccatta tatagaaagt taaagattat cgtttgttag
    12121 ttgataatta gaagaacatt taatgctact gcataaattt tatcctaaat aatatcaagg
    12181 tgatttaatt tatgtattct aatgtaactg tagataatca atttataact aacttagtgt
    12241 aatttagatt taatttatta ttctaatgta accccaatat attgttaagg tctacttaag
    12301 atgctcaaaa ggctatgctt aatttattgt tatataattt gtagagtggt ataatttaga
    12361 agtgatacta tatagatata tagggggaaa atatgaaaat atatgatata ttaacacgtt
    12421 tttatattca agatatagaa aaagccatac ctttttatga gaatctttta aaagaaaaat
    12481 gttctttgcg tttttcttat aaagaagttg ggttggaatt agcgcaaata ggaaatgttt
    12541 tactattatc tggttctgat gatgctctta aaccatttat agaaacaaaa tctactttta
    12601 tggttgattc tgttgatgaa tggagaagtt atctattgga taatggagca gttgtagtaa
    12661 gagataagaa aaaagttcct acaggatata atatgacatt gagacatcca gatggtacaa
    12721 ttatagaata tgttcaacat acaaagcagg attagttaaa aatagatttt actactgagc
    12781 aactaattag aatctattct tagacgtaca atttattatt tttggtatta atagattata
    12841 agtcaaatta taatatttca aaatagaata gaaatttatt tacataaaag agagtgttta
    12901 aaaatgaact ttttagttca cgaagcactc ctttttgtat gaaatcaaat acatcaatat
    12961 ataaaacaaa ctgatgaaga gcttaaatta aaataaaaag attcataaca ggaccccatt
    13021 aaactttaag atagggtaag tttgtaatca tattaaataa ttgtaaaaag ctgtttttag
    13081 tcgtataata taaatattta tcatttaatt aaggaatcta aaaggaatct aataagaaga
    13141 aaataataaa taacttttta gatttactaa atgaaaaatt attgaaaatt aaaggattag
    13201 agtctttaat cagaaagtaa aaagtaatat attaaattta tgaattgaaa acttaaacaa
    13261 caaatttgtt tataaaatag caagattcat taaatataac taagtaataa ttgatataat
    13321 gttgcatgga taaaaggagg gaagctgcaa tgtcttacaa agtaattaat gtcataactg
    13381 ctattgacta tatagaggag catttatcag aaaagctgga tttagatata attgctaatg
    13441 ctgttcatta ctcaaaatat cacctgcata gaactttcac aacttctgtt ggtctaacaa
    13501 tgcatgatta cataaagcgg agaaagttga cagaagcagc aaaattattg gtattttcaa
    13561 agaaaccaat tattgaaatt gcattgatag ctggatatga aagtcaacag gcattcactt
    13621 ctatatttaa agctatgtat aaaaaatctc caaataaata taggaaagaa caagaatttt
    13681 atcctttgca gctaagattt gttttgaaaa aagataacct attttcaaag aatgtactag
    13741 agttggaaaa agaaattaaa cttgcaagta tgtctgacat accattatgg atgaatttag
    13801 tgagattagt catagatgga tttccaaatt tgcaagagga agaatatata tatcaattag
    13861 aacagtatat tttagaagaa agagctttaa tattaaaact taataatgtc gctattgcaa
    13921 atatggtgtt taatagagaa actagaagta ttgatttttt tggaatacat ccacagtatc
    13981 gtaattcaga cattgcacaa gtgtttttga agaaagttat tgaagatttc ttgatagata
    14041 cagatatctc aattactacc tttcgagagg gtgacaaagc tgatacagga catagagata
    14101 tgataaagaa gttggggttt gcagaagcag agttgcttgt ggaatttgga tatcctactc
    14161 aaaaatttat tcttccttgt gtagaagaaa gagataatag acaaaattaa ttgtagatta
    14221 aatgcaaagt ttaccacata ttttttgttt gaattatttt atatataaga gtatttctgc
    14281 ttttaatgta ttttgcttgt aagtgtactt atggtaataa atatagaaag atttagtttt
    14341 ttaaaaaaag aactttaaag ctaaaaagct aacatttaag ttaaatagca agaatgatta
    14401 aaaactccaa aacacaatta agtataatga ttcagtgtaa gtacttaatt aacaaaggag
    14461 gatttaaatg tctgaaaata aacaagtagt aaattgcgat attattgcat ccaattctaa
    14521 acataatatg tgtgaacatg atgtagagtt attaactttt tttccagatg agattgcaca
    14581 tctaatagag ataaataaca agctagacaa tgcttttaag aaagctgaaa atcttgttga
    14641 caagcttgat aaggattata tggacgctaa aatgtatatg gtaaaaaatc gtggtgaaat
    14701 agacccacat gaaatgtttc aaaatgaaca aggactaaaa caaatagata actatggggc
    14761 ttttatggta aaagttcgag ataagattaa taaaatcaaa gattctccat actttgctag
    14821 aatagatttt aggctgaaag atatggatga tgaatctaag tattatattg gtagatttgc
    14881 atttgattat gaagatgagc ttataatact tgactggcgt tctccaattg ccagtatgtt
    14941 ttatgattat gaaatcggaa aagctggata tgatgctcca ataggatggg tggatggaga
    15001 aataacaaga aaacgacaat ttaaaattaa aaatggaaaa ttggaatatg cactagaaag
    15061 ttccattaat atacaagatg atattcttca aaaagaactt agtcacactt cagatgaaaa
    15121 aatgaagtca atcatttcaa caatacaaaa ggaacaaaat caaattatta gaaatgataa
    15181 agctgataca ctgattattc aaggggtagc tggttctggg aaaacatcta tagcattaca
    15241 cagaattgct tttcttcttt atcgatttaa agataaaata tcagcaaata atgtaattat
    15301 cctatcacct aataaagtct ttggagacta tatttccaat gtccttcctg agcttggaga
    15361 agaaccatta tgtgaattaa gttttgaaaa tatagcagag gtacaattag atagagttat
    15421 taattttgaa agtgagaaag acccattaga aataaatgat gctaaatggt ctgaaagagt
    15481 gcgttttaaa tctacacttg attttgtaaa acttattgat gattatataa aacaaatgcc
    15541 aaataagatt tttattccaa aagactacac ttttggaagt tttacagcta aaagtgactg
    15601 gatacagagt agatttgagg catataacag atatccagtt aagaagagat tagaaaaggt
    15661 agctgaagac attcattata aatttgaatc agataatatt atggaagaag atttaccaaa
    15721 ggtaaaaagt atacttaaaa gtttgaatgg aatgttgaca ataaaaaata ctcttacact
    15781 atataaggat ttctttaaac aaatgaatat atcaaacatg tttgtgatgg cagaaaagaa
    15841 aacacttgaa tggtcagatg tatacccatt tatatatatt catgctgcat atgaaggtat
    15901 acaagaagat aagattataa ggcatattgt tattgatgaa atgcaggact atacacctat
    15961 tcaatatgct gtaataaact tactttttaa gtgtaaaaag actattcttg gagattttgg
    16021 acaattggtt aatcctaatc atactcatac attagatgat atgaaacagc tatataatga
    16081 tggggaacta gttatgttga acaagagtta ccgttctact tttgagatta ttaatttcgc
    16141 caaaaagatt caagatatat cttcattaga gcctattgaa cgacatggtg aagaaccggc
    16201 tttattaaaa tgtaataacg agcaagatga aataaataag ataaaaatag aaattgaaga
    16261 atttaaaaag agtgataatg cgactcttgg tattatatta aagacagata atgacgctaa
    16321 agctgtatat gatgcgttaa gagaagaata tagtgttcac ttaatatctt ctgaaagttc
    16381 tagctttaca aaaggagtat ccataacttc tattaaaatg tcaaaaggtt tagagtttga
    16441 tgaagttatt gtaccttcag taaataataa aacatattat agtgattatg accgcagttt
    16501 gttatatatt gcttgtacac gagctatgca caaacttaag ttaacatatg ttggtacatt
    16561 aactcaactt ataagcatgt agattatatc ttatatgcat ttattatcgt aaatataaat
    16621 tgggacaaag attcatcaat aaatcaacaa aaaataatac ttataaattt ataaaaaatg
    16681 aaagctataa attacatttg aaattttata aaattttgtg atttatagct tttataatat
    16741 taatttcttt taaaataaag ttaaacttta aatttatgaa ataaaacaaa gtagtaatat
    16801 atatgtaata gttcttaatt ttttaatatt agtattatat tatctatata tttattgatt
    16861 ttctaaggaa atctgatgtt atagtgcaac ctctttcaaa catttctagt ggtgtacctt
    16921 caaatacgac ctgaccaccg tttttgccac catcaggacc aatatcaatt acccagtctg
    16981 cttgtttcaa tacatccaaa ttatgttcaa ttataattac agtattacct ttatcaacta
    17041 tatgctcaaa aatcaacata agcttttcaa tatcagaacc atgaagccct gtagttggct
    17101 catctaaaat atatatagta ccttttttgt tgaggttttt agctaactta attcgttgac
    17161 gttctcctcc agatagtgtg ctaagtggtt gacctaaggt catataaggt aaaccaacat
    17221 ctttcattgt ttgtaagatt ttacatagct tcttttctgt aaaaaattca agagattcat
    17281 ctactgtcat agatagtaca tcaactatac atttaccttt atataatttt gacaaaactt
    17341 cttcactata tctagtacct ttgcaagctt cacatgtatt aataataggg tccatgaata
    17401 caagctctgt aacaagtact cctttaccat cacaaacagg acaagctccc tttgaattaa
    17461 agctgaataa accttcttca acattatttt ctttagcaaa tacttttctt atctcatcaa
    17521 agaaaccaag aaatgtagca ggcatagaac gatttgtagc tgtaactggg ctttggtcga
    17581 ttttaataac atctttttcg tatttttgtg caaaaacacg agagattaaa gtactcttgc
    17641 cagaacctgc tactccagta acgacagtca taattccaac tggtaaatct acatcaatat
    17701 gttttagatt atttatgcaa gcatctctaa ttggaagggt accttttgga tgacgagagt
    17761 cagtttttat aggtaatgat agattcattg catttgaagt aagagtgttt gctaaaagta
    17821 atttatcata agagccttgg aatacaacat gtccaccctg agtaccagca cttgggccaa
    17881 tatctataac ttcatctgct atggagataa catctttatc atgctctact acaaggacag
    17941 tatttcctct atctctaagt ttttttaata gctgattcat acgatgtaca tctcgtggat
    18001 gcataccagc actaggttca tcgaaaatat aagtcatacc tgtgagactg ctccccatat
    18061 agcgtacaag ttttaatctt tgagcttctc ctccagatag agatgatgat tctctattta
    18121 gatgtaggta agggagacca atatcaatca ttctttttaa accatcagta agagtcttta
    18181 ttacagtttc agttctttta tcttttattt gtaacaataa atcataaagg tcagtaagtt
    18241 ccatattgca catatctgca atcgaatatc ctaaaatatt acagttaaga gtttctttat
    18301 ttaatctttt tccatgacat acaggacatt ccttatctgt tataagtctt tctgattttt
    18361 cttttgtgtg tttgctcata ttgctaatat ctcgatttag atatgtttga ttgtatttgt
    18421 taaaaatacc ctctattttt ggattttctt gttttccatc tttatttttt gaaccataaa
    18481 gcaataaatt atattcttca aaagaataat cttttatagg tttatctaaa tcaaaaagtc
    18541 cagactctgc atactgcttc cagtaccatg aattaggttt aaataaagag tcatttatac
    18601 atcctgtatt ccatgaattt tcaggattta taatagattc aatattgatt actttaactc
    18661 taccaatccc agaacaattt ttacacatac catttgggtc attaaaagaa aaatatgaag
    18721 ttgttcctat gtagggttct ccaattctag aaaataataa tcttaatgat gaatacaaat
    18781 cacttatagt gccaactgta gaacgtgcat tgccaccaag aggagtttgg tctacgatta
    18841 cagaagcagt taaattatca atcagctcca cctgaggttt tctatattta ggtagcctat
    18901 ttctgataaa tgaagaatat gtttcattca tctgcctttg tgattctgct gcaattgtat
    18961 caaatacgat actggatttt ccagaaccag aaactccagt aaaaacaaca attttatttt
    19021 taggaatttt aaatgatata ttttttaggt tattttgtgt aagtcctttt attataatat
    19081 ttgacatgaa tgttaaatcc tttctgagta cattttagta aagcaattat attatgtaac
    19141 catattataa taaaattaaa atctactttc ttgatatatt ctgctatgtt atagaaggta
    19201 tttctaaatt taaccaatcc catacaatct gaataaatga tagttgataa tagataaaaa
    19261 agtatataat agatactcgt aatttgataa gtacaaagat aattatacaa ttaaaacgac
    19321 ttgacattta aaacttacta ttgtaatatt taaatattac aatagtaagt tttaaataga
    19381 gaataaataa ttacctcaca aactgaaagg aggacataat gatactaata gttatattga
    19441 gtatattcat catagcctca atcaacataa ttatattagg taaagaaaat ataagagata
    19501 tagttatccc tttaactcta tttattgtaa gtgatttttt atttttgtgt ggaaatataa
    19561 tatgtagggg aggagtaatg ctactattta taggtttatt tgcaatacca ttgatgctaa
    19621 ttgcatttat atggatttgt gtacatatga ttaagcttta ctataaaaaa gatttaaata
    19681 aaagaaaaat gattggatgt atatttagta tctttataag tataggtact atatttattc
    19741 ctagtccaag tgaaagtttt agatttaaac tttatgaaaa agattatagt gtagtagcta
    19801 aagctatttt gaaagtttat gatgaaaaaa agttaagctc tgaatctgtc ttttatattc
    19861 aatctgataa aaatgaattg gagaagatat tttctaaaga agtaataaaa aaaatgaaaa
    19921 aactaaacag aaatttagat gtagttaaaa tttctgggaa tagaaattta gtctattttt
    19981 tctttggagc tgaattacaa tctattgatg gtattgtgat ttttaaagat ttaaaacatg
    20041 ttaaaccaaa gttagatttt ataaatagaa gtattcaagc tcctagattg gagtatatta
    20101 caaataatgt atattatttt aaaggtgaac tataaactta tgtagaggag atggtataaa
    20161 gtatgaaaag aaaaaataat tttatttgga tagttattct tctagttgtt gtagctatgg
    20221 gagtatatta tgtaggaaga aagcataaca acacaaaaca aaaacatgat aagaatcata
    20281 taaactctga attaaataaa tccaaagttg aaaacaatga taaaaacaaa aaagaaaaaa
    20341 ttaatatggt tgactatagt gactgttttg aaggtattag tggaggagct attttttata
    20401 atactaaaaa taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac
    20461 catgttctac ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaact
    20521 caaaagaatc agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa
    20581 atcttagttt agaagaggca tttaaggagt cttgtgtttg gtactacaaa aaattgatag
    20641 ataaagttga tgctaaatct gtccaaaaca ttttagatga tttaaagtat gggaattgcg
    20701 atattagcga atgggaagga gatttaaaaa atggaaaggg acatttaaat ggattttggt
    20761 tagaatcttc attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg
    20821 agggagatac aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag
    20881 atgtaaatga taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa
    20941 ataaacgtgt ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt
    21001 ttgctattaa atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg
    21061 caattaatat aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa
    21121 tattatttac ctatagtctg taatatttat taaataattt tactaaattg agtctatata
    21181 gagcaaaact cctttagtac ataaaatact tgctattgat tattaaattg aatacctata
    21241 gatttaaagt taataccttt taaagactat gtaatatatt aaaagtataa ataaaatata
    21301 ttgcatagtt tttgttttat attataattt taaataatgt gcaaaaacta aacaatattt
    21361 ttaatagtaa tttatatttg tattcatgtc actataatgt cattttaggt tgctaaaatt
    21421 taaccataaa ggagatgatt gtatatggaa atattaaaat gtgaaaatct cacaaaaata
    21481 tatggttcaa atcaaaccag agtgacagcc cttaataatg taaatttatc agttcaaaaa
    21541 ggtgattttg tatccattgt aggagcatct ggttctggca aatctacatt gttacatctg
    21601 cttgggggag tagatagacc tacatctgga aagatttatg ttgaagatac agaaatatca
    21661 agtttaaagg aagaagctct tgcagttttt agacgtagaa aagtaggtct tatttatcaa
    21721 ttttataatc ttatacctac ccttgatgtt agaaaaaata tattattacc catgttactt
    21781 gataaaagaa aggtggatga ggatagattc tctgagattg tatctatatt gggattatct
    21841 gatagattaa accatcttcc aagtcaactt tcaggtggac aacaacaaag ggtatctata
    21901 gctagaagtt taatctatag accagctatt ttattagcag atgagccaac tggtaatctt
    21961 gatagaaaaa attcagaaga aattgtagac ttgttaaact tatcaaataa acgatttaat
    22021 cagacaatac ttcttattac tcatgatgag aaaatagcac tagaggcaaa tcgtattgta
    22081 actatggaag atggagtgat tgtctctgaa aaggtggtga agaaatgaat attcttcaag
    22141 aatacacctt agactttgta cgacgcaata aacgtagtag tatagctata atgttagcta
    22201 tattacttac tacaaccatg atgtcttctt tgtgtggact gttgtatacc atgtggacag
    22261 attttataag actttcaatt gagaaagaag gtaactggca tggtgaattg tttgactata
    22321 cttatggaag tgacttacca ttaattgaaa atttttcttc agttgatgat gtaatggtaa
    22381 aaggacaatg gtatgtaggc aaaattaatg atgagaaaag agattatata atttatagaa
    22441 atgctacatc agagtattgg aattctatgc cagaaaagga tactataata gagggtcgtc
    22501 gaccaaataa tgctaatgaa attgcattat caaaacaata ttttgagaat aatccaagtg
    22561 taaaaattgg ggataaaatt actcttccaa taggagatag aatcctaaat ggaaagcctc
    22621 ttgaacccat agctcaaaaa tcgaaagggg aaagttttaa aaaaatttca gagactacat
    22681 tgacaatagt aggaaaggta gatgtttcca cttcatcagt aatacctgca tatacagctc
    22741 ttggatatct tgatagaaca agtataaaac ctaatgatag tataactgtg tacttgcgat
    22801 ttaaaaatat aagagacaca tacaaggaac ttccaaaact tgctaaatca ttggggtgga
    22861 agactaatga atatggaaaa tacaatctta aatataatag taattatctt ttaaaaatgt
    22921 tagtcttttc cccagaacag aaagcatcta tgagcagtat atcaaaattt tcaactccta
    22981 ttatgtatct tacaatagca atttttacag tagctgtgtt tgtaatggtg atttacaacg
    23041 cgttttcact atctgcaaat gctagactta ctcaattagg gatattatct agtgttggtg
    23101 catcaccaaa acagattaaa atgtctgtag tgtttgaagg ttttttgctt acaattatac
    23161 cattgccaat aggtttattt ttaggatggt tattgtgtaa taggcttata gtttatatta
    23221 attcagttaa ttatcatact gatgttccag aagtggtatt tacttatggt attcctgctt
    23281 ttctgccagc agttttactt acaatagtaa cagtctggat ttctgcactt ataccagcac
    23341 gaaaagtatc aaagatatct cctatagaag caatacgaca aggagatagt gttaaaatca
    23401 aaaagtcgca taaatattca ttaggaaaga tatttggaat agaaggagaa cttgcatcaa
    23461 acgcactacg tgctcgtaaa aaatcttatc gtactgcaac aatatcttta acattatctt
    23521 ttttattact tacatgtttt ttgcatataa ttgcaaatca agaaggagca aaagctgttt
    23581 atggaagtga taattataag gaccaaagag atatagcagt ttatttggaa aatagtgaac
    23641 ctgtagagtc tgctttcatt gaaaaattag ataatacaga aggtgtaaaa agttcactat
    23701 attatcaaaa aatgtctgct tccacttggc tgactgaaaa agatgcatca aaagaacttg
    23761 cgaaaaatgg aggatttaaa agtatagttt catctaaaaa atactctcct atagaacgtg
    23821 atggcaagtt tcgtataatt tcaaatataa ttgggcttga tgataagagt tttacagaat
    23881 attgtaggca aatttctgta gacccaaagt tattttatga tacaaaaaat ccaacctcaa
    23941 ttgtatataa taaggaggaa gatattaatc gtagtacacg tagaaataaa gtatatatag
    24001 attatttaaa tctttcaata ggagataaca ttaaattaaa tgaaaaagta tatgacgaag
    24061 ataaagggga ttatacttac aacatgaaag tgggagctat tacaagcaca cttcctaaaa
    24121 cagtagatac tagttcaaaa tatacattaa tgcaaatagt gccaatgtct atggtgcagg
    24181 aaatatctaa aaattatgat gaagtaagta ggttacgttc aaataggctg ataggagtat
    24241 ttaaagtaaa taatcgtgag gacattccta gagtaagaga aaaattagag tccatttata
    24301 ataaagacta tggttcagga gattatagta ttgaggatgt tttagaaagt gaaaaacaaa
    24361 aggctagtgg aataaagacc atgaatttaa tagtaggctt tattacaggt ttattggcta
    24421 tgattggatt atcaaatgta cttgctacag tgtcaggaaa tactagaagt agacgacaag
    24481 agtttgctat gttgcgttct gttggattat ctccagaagg cataaaaaag atgcttgttt
    24541 tggaaggttt gttcttagga attacaccat tactacttag tattcctgtg caaataggaa
    24601 tagtgtatgc ttttctacgt ataaatgaaa tatattttat agagtattta ccatttgcac
    24661 ctatatctac aataataggt tttactatat tgatactttt tattgtaata gcatcatata
    24721 tgacaggata caagcagttg aagaatgaaa atatagtaga atctataaaa aatgaaacaa
    24781 tataaacaaa agctatttga acttatttaa aggtttcgat ttaagactac ttacaagtat
    24841 agggatagtt aatttgtttg tttaataaaa ccttaaatgt aaatagaagt atattactaa
    24901 aacaatagat aacatagaat atagatatag ctggattata ggtattttac ctataatcca
    24961 gcattttatg tgtaaaattt aattatgaat cttgcaccac cattatggca attttctgca
    25021 tgtagagtac cattttgacg ttctataata gactttgata gagcaagccc tataccaata
    25081 ctgtccttac tagcattttt accacgataa aatctattaa aaatatatgg aagttcttcc
    25141 tgagcaaacc ctttaccatt atcttctatg ataatctcaa catatagagg attttgacta
    25201 taataagcaa ctatatatcc accatcttct atatgctcac tacaattttt aagaatattt
    25261 aaaagagctt ctaaagtcca attaatatcg atttcaaatt ctaccgaagg agaagcctcg
    25321 atagtaaatg tctgatgttt ttctcttaaa gaaccttcta taggctcaat tgcataagta
    25381 agaagagaat gtacatctat aagtttgctt tcaaatatta aagtacttgc atccaatttt
    25441 gataatgtaa gtaaagatga aactagtctc tcaagtcttg agatttgatt tgtaagtctg
    25501 ttaatataat ctatttcttc atctggacaa ttttctgaaa gtaattgtgc cataagtgac
    25561 atagaagtaa ttggagtctt aagttgatga gcaatatctg caagattatc tgaaagagtt
    25621 tgtctttctt ttaaagcaaa ttcttttgaa caacgaagtt ccccaactgt tttatatatc
    25681 tcatcttcaa ggtgagaaaa ttcatcttca catcgtaaaa gtacagactc ttttcctaaa
    25741 ttcacttctt ctaagtattg agtaagattg tttattctac tcagtttttg cttatttgtt
    25801 ctatgtttga ttatatatac aactgtagcc attgttgaaa ttataccaaa cgatatcact
    25861 agaaaataaa aaaagttttt actccaaaat gtacttttag aatatccatg ttttgataaa
    25921 atttcatttc ctgttttaaa atccttatca ctagaatttt tcagtgattt tagtatactt
    25981 tctgtagact taggattgta ctcatatgat gcaccaagag ccttagacag tacattgtat
    26041 tcattaaaag aagttatagt tgttagaatt acacttaata aaaaagttat aattaagcag
    26101 aatattataa aaacaactgt actgttgata tttcttggag ttttcactag ctgtcctcca
    26161 ttctatatcc tatgcctctt aatgttttta tacaaggtgg attttttaat ttttctctaa
    26221 gacgctttat tgtaacagta agagtgttat catttacgaa attaccatct acatcccaga
    26281 gcatttctag taactttgta cgggtcatag ctctattttt attttccata agacttagaa
    26341 gaagtttata ttctcctgct gtcaaaagta cttcaacatt atctaaatat gctttgactt
    26401 caactttatt caagcttata tttccacatg taatagtatt gttagtattt gaatgagttc
    26461 ttctaagaac tgcattaatt ctggattgaa gtactgagag cagaaatggt ttaaccacat
    26521 agtcatctgc acccatatct aatccacgta taatgtcttt ttcatcatca cggactgtca
    26581 aaaatatcac tggagtatct tctatacttt taacatattg gcaaaactcg tatccaatac
    26641 catcaggtag atttaaatca agcagaatta aatcaatagt tttatcaaaa ttttttttag
    26701 cttcagcaat cgtattataa caacatacag tgtgattttt cttttcaagg gaagttttta
    26761 ttccaaatgt aattgtatca tcatcttcta aaagaagtat tttagccatt tacttttagt
    26821 cctttcagta atttagttat ttcattaact aagttattag ttttatgttt aatatttttt
    26881 atgttagaga tagaagttag agatagaaaa aattatcatt taagtatata aactttaatt
    26941 tataaactta ggtttatgct ttaattgaat ctattaaaag agcaagagtt gtttcaataa
    27001 atttttcctc atctatatta aagttgttca ggtatgactt tcctgttaca gatagtaaat
    27061 taaaaaatcc tgtaagtgta aatattgatg aatacatgag agtaggtatt tcaatatcat
    27121 gcctaatact tctatcattt tttccaattt caaacattcc ttgtatttca tcaaacataa
    27181 atttattaaa agaaaaaaac ttttctttaa acagcatatc agtatccgat ttttcagagt
    27241 ttacaatacc aatcattccc ataaggcata atagagaagg ttctacagag taaaatttat
    27301 gaaaagcaaa gtatgaaagt tttatttttt caaaccctgt tttgcctttt gcactttcta
    27361 ttttaacatt atcccaaagt cttttatatc ctcttaagac aacagcaaag aataaatctt
    27421 ctttacatga aaagtacttg tatatggtgc gtttagtata ttcaacttct tttgccaact
    27481 cattcatagt tgtattctga acctcccatg actaaagtca cagggttcct gcttcataga
    27541 attttgcata ctaaaatatg tcttactaat tctccacagg ccatccccgt agttcctacg
    27601 gttcttacat aagttattta ttttgaatcg ttattagtct taatccttct tttaatatat
    27661 ttatacttgc atttatatct ctatcgtgag tttcattaca actaggacaa atccattctc
    27721 ttatatttaa gttcttaact tcctcgtttt tatacccaca tttattgcat atttgtgaac
    27781 ttgcaaagaa tttacctact ttatacaatt tgtctaccat accaattagc cttgtattct
    27841 aattgacgaa taaattctga ccatgataca tcagaaatta agcgagatag tttatgattt
    27901 ttaatcatat tctttacttg taaatcttct atgcaaataa tatcattctc ttttattaat
    27961 ttagtagata
//
LOCUS       DAMJVX010000031        27597 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMJVX010000031 DAMJVX010000000
VERSION     DAMJVX010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27597
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.033"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(81..998)
                     /locus_tag="PC407_001300"
     CDS             complement(81..998)
                     /locus_tag="PC407_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HDF2496755.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIETVKEFWE"
     gene            1260..1637
                     /locus_tag="PC407_001301"
     CDS             1260..1637
                     /locus_tag="PC407_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2496756.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            complement(1793..2242)
                     /locus_tag="PC407_001302"
     CDS             complement(1793..2242)
                     /locus_tag="PC407_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2496757.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(2420..2824)
                     /locus_tag="PC407_001303"
     CDS             complement(2420..2824)
                     /locus_tag="PC407_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HDF2496758.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            complement(2937..3803)
                     /locus_tag="PC407_001304"
     CDS             complement(2937..3803)
                     /locus_tag="PC407_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2496759.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRSTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            complement(4373..4717)
                     /locus_tag="PC407_001305"
     CDS             complement(4373..4717)
                     /locus_tag="PC407_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDF2496760.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            complement(4906..5523)
                     /locus_tag="PC407_001306"
     CDS             complement(4906..5523)
                     /locus_tag="PC407_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2496761.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKNLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            complement(5528..7699)
                     /locus_tag="PC407_001307"
     CDS             complement(5528..7699)
                     /locus_tag="PC407_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDF2496762.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNED
                     TINKILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVS
                     REASILRLNGYSKLDIIARVIKNIFRIMILSSVTALLIYIIYILKLNIGVRACLYFAM
                     FSIICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSI
                     NNCILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLV
                     EEKDAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGK
                     EIFNLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKT
                     KKSDLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIIDIDNIDDSFYLSYISRCV
                     YFNSKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIA
                     ISNLMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRV
                     DLSNNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            complement(8114..8746)
                     /locus_tag="PC407_001308"
     CDS             complement(8114..8746)
                     /locus_tag="PC407_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HDF2496763.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINLENLHIWLENGVDVCGFGGLLTKGTIEDITENAKKIREIIKNIREI"
     gene            complement(8852..9757)
                     /gene="dapA"
                     /locus_tag="PC407_001309"
     CDS             complement(8852..9757)
                     /gene="dapA"
                     /locus_tag="PC407_001309"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2496764.1"
                     /translation="MKYKGIICAMITPFDEKQNINSQATCKLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVIDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEVTQNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            complement(9762..10361)
                     /locus_tag="PC407_001310"
     CDS             complement(9762..10361)
                     /locus_tag="PC407_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2496765.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIVLQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            complement(10419..11690)
                     /locus_tag="PC407_001311"
     CDS             complement(10419..11690)
                     /locus_tag="PC407_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDF2496766.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            complement(11717..12004)
                     /locus_tag="PC407_001312"
     CDS             complement(11717..12004)
                     /locus_tag="PC407_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2496767.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            complement(12019..14106)
                     /locus_tag="PC407_001313"
     CDS             complement(12019..14106)
                     /locus_tag="PC407_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2496768.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPYNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIYLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            complement(14057..14212)
                     /locus_tag="PC407_001314"
     CDS             complement(14057..14212)
                     /locus_tag="PC407_001314"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496769.1"
                     /translation="MKLKTIKFSNKISIVEVMKESNDNMKTNLNKKVGVNGSGQRNCI
                     NSPDFIK"
     gene            complement(14673..15527)
                     /locus_tag="PC407_001315"
     CDS             complement(14673..15527)
                     /locus_tag="PC407_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HDF2496770.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            complement(15559..16071)
                     /locus_tag="PC407_001316"
     CDS             complement(15559..16071)
                     /locus_tag="PC407_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDF2496771.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDKKKGN"
     gene            complement(16064..16288)
                     /locus_tag="PC407_001317"
     CDS             complement(16064..16288)
                     /locus_tag="PC407_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496772.1"
                     /translation="MNEKPSFKQIEIILAVIFLVLTYYKIGLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            complement(16348..17301)
                     /locus_tag="PC407_001318"
     CDS             complement(16348..17301)
                     /locus_tag="PC407_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2496773.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLESGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            complement(17329..18717)
                     /locus_tag="PC407_001319"
     CDS             complement(17329..18717)
                     /locus_tag="PC407_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496774.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPNNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKAGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            18990..19871
                     /locus_tag="PC407_001320"
     CDS             18990..19871
                     /locus_tag="PC407_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2496775.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            complement(20058..21050)
                     /locus_tag="PC407_001321"
     CDS             complement(20058..21050)
                     /locus_tag="PC407_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDF2496776.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKKIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(21055..21795)
                     /locus_tag="PC407_001322"
     CDS             complement(21055..21795)
                     /locus_tag="PC407_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HDF2496777.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLVGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGDTKVEDIGVLYKIMKELLG"
     gene            complement(21860..22969)
                     /locus_tag="PC407_001323"
     CDS             complement(21860..22969)
                     /locus_tag="PC407_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HDF2496778.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFL
                     IIEELVNKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEINFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDNIYNRIKNIVES"
     gene            complement(22987..24120)
                     /locus_tag="PC407_001324"
     CDS             complement(22987..24120)
                     /locus_tag="PC407_001324"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HDF2496779.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNHIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGEFY
                     EDTKKYKTNVYALINIAKQGITSQDELSNMMNIDEYELKNAVRKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEAKERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGNSIEENE"
     gene            complement(24122..25216)
                     /locus_tag="PC407_001325"
     CDS             complement(24122..25216)
                     /locus_tag="PC407_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2496780.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMVSKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNEYQVVFNVVAKEKTSEVEKIIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNKNIFKRGDVVIGNDDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            complement(25353..26654)
                     /locus_tag="PC407_001326"
     CDS             complement(25353..26654)
                     /locus_tag="PC407_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2496781.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKATLDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            complement(26878..27579)
                     /locus_tag="PC407_001327"
     CDS             complement(26878..27579)
                     /locus_tag="PC407_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2496782.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
BASE COUNT         8950 a         4926 c         3048 g        10673 t
ORIGIN      
        1 ttataaatta ttgtcaattt attttttctt aaaaataaaa taattgcata atttatttca
       61 aattatgcaa tttataaaat ttattcccaa aactctttta ctgtttcgat tatctcacta
      121 taaggcaata aagtcgcttc gattattgat ttatttgtct tttctaaata atacttaatc
      181 atataatatc cacaagcata tcctgcacaa taaggcaacc ctactggaaa atatccttgc
      241 attttagcta tatcatcacc ataaagataa gatgttatat tttgaaatcc agtttctttt
      301 aaagcacttt ttattattgg ctttatataa gtatttaatg tttcgatatc tgttctactg
      361 acccaaggtc ctaacatttc ctctccaaac atccatgttg caaaattttc tgcaagacct
      421 tcatttatca tcatttcttc taatgttata ttattattcc actctataaa ctgaaatcta
      481 atattgtgat tacattcatg tgctattaat actggtaatc tattgatagt atattcatta
      541 ggaaccaatg atagaaatat atatccagga atcccaccat caccccagta tccatcactt
      601 aatattgtat aaggactatt tggattcgcc aataatattg aatatttata gtccttaatg
      661 tttaaatcat acccttcttt tataaaagca tttatagagt tttctatggt ttctttgcaa
      721 gtgccccaaa ttttatcagc agataacaca tttatattct ttttttgctt ttcatcaata
      781 tccttaggcg ataaaacccc taacatttca cttgctataa ttacatcata tccacctttc
      841 tgtctagcaa ctattggaac attcatacac ttccacttaa attcaaaagg cttcatcaat
      901 tcatatctgt atatatcttc ttttttgttt attggtgcat ccattatctt cttgtatatt
      961 ttatctgagc gaatttctat aatattcata tttaccatct cctttaagac aaagtatata
     1021 caattacgta gcgtcaatgt caatatacat tttttataat gtcaccttaa tctttttatt
     1081 atattttatc ctgtaaacct tccgtacata taacattttt ttaaattaat aaatttgtat
     1141 aatataataa taaaaaatta tatacttcaa ggtttcccta tgatacaatt tactaaatca
     1201 taatttagat tatcaggtgc aactataaat tattctatat caaggaggat atattggtca
     1261 tgctaactcc atatttaata tttaatggta cttgtgaaaa agcatttaat ttttatgctg
     1321 aggctttcgg aggaggaaaa actatatttg cgcgattaga cagcaatcca aacaatccta
     1381 ttatgcacgc aagtgttact ttcacaaaat acgaaggttg tataatgggt gcggatacag
     1441 acaagcctgt tgtaatttct ggcatggcga tttgtgttgt tctaccatct cgagaagcga
     1501 tagaagaaat atctgtaaaa cttgccgagg gtggtacact tgtacaagaa tttttaccac
     1561 acccaccacc acatcaaaat gatggcgctg ctgaagtact tgataggtat gggtatactt
     1621 ggtatttaag tacatagtaa aaataaaaaa actacatcta ataatcataa tatagaacag
     1681 ataaatttca atttaatagt gtagtaaaaa agtcgtgtcc taagactgtc acgactttta
     1741 tttgtataaa aatttaagta aatatttttg cgaataagtg agaagtataa ctttaaatta
     1801 atttcatatg tagtattgga aatgggttac cttgtccatc tatttcagac ctatcaaaca
     1861 ctttaaatcc taagtgaata taaaatccta cagctttttt attctgctca tttacatcaa
     1921 catattttac attgtattta tctatagcat attttattaa ttttttacca ataccatttc
     1981 ctctatattt atcttctata aagagcatct caattttatc tttatctatt cctataaagc
     2041 cctttattat atcattatct tttatacaaa ctatattttc tacttcttta agtccaattt
     2101 caactaatgg tatcaaagat tcaatatctt tttctgttaa aaaatcatgc gttgctctta
     2161 ctgatgattc ccagacttgc aacatttcgt catattctgt tgaatttatt tgtttcattt
     2221 caagaattat tccagatttc ataaaatttc ctccctatat cacttttaat tgtataacac
     2281 ttttttatac aaaacttaga aaggactacg aaggttaagt atcatagtcc taattaagtt
     2341 taaatattat ctattatata tttttaacat ttattatata ctataatcta gcaaatttac
     2401 tacaataatc catatcagtt tatactaaag gtgcttctat ttgagtagta aaaaggaatt
     2461 tatgacaatg accatcatta acagtagttt ctccacatat taagtgaatg tgtttatcag
     2521 ttccaggtat tctaattgct ggccctgttg taacacatat cttatggaaa tggtcaagga
     2581 aatctacatt atcatttatt ttatgaacat gatttgtacc tccatttatt ggtatagctt
     2641 caccagtaac ccctgctgca cggtggttat gtctatcctc acattcttct gctaacttaa
     2701 cactttctga atattcatga acatgtgtca tttcctcata atcattatag cacttgttgc
     2761 acttattgta tttataatca tcatacacat aatctctttc acatttataa ttttgataac
     2821 tcatttttat atctctccct atattatatt aagaaattct tgtaaagaat ctctaatgta
     2881 taatatgaat taaagtcaaa aaagttcata ttttgtaaaa ttatgtatta atttaattat
     2941 ttctcccagt atttagggcg tattagtgaa gctggtaact tcgtactaga atctcctttt
     3001 gcagtattta cttgcaattg tgttaaaaat atactatttt caagattagc acctctaata
     3061 tcacaatctc ttaagtctgc tcctattaaa tttgccccac tcaaatctgt acttcttaaa
     3121 tttgctgcta ttagaaatct acctcttaaa tctgctccta ttagatttat ttttctaagg
     3181 tctgctccta ttaaatctat atttttactt aattttttca tatttttaag agtagaagtc
     3241 ttgataaatg atttaacctt tcccatcaca gactcactcg cttgtgataa taataaatta
     3301 acctttgttc tatgtgatat gatatccaaa ttaattatct gttctgggct catattagtt
     3361 atcttttcag tttcttcaat tatcaaatca atttttgatt tgattgtatc tgatatattt
     3421 aatacagatg cttctttaag ataccataac atttcatgta attgtctcat aattaaaaac
     3481 acttcagaca tttgatttgt taattctggt gattgcatcc agtctatccc cttatatgtt
     3541 acttgtgcta ctttttgacc tgaaccaaaa caatcatatg cagtacaccc tttaaaacct
     3601 cgcttcataa gactattatg tacagaacat ttaaaatctg gttgtaaatt aatacagggt
     3661 tttcctgatt ccttatcgat aggaaatcca tctgaagcag aaaaataaag agctacacaa
     3721 caaagtccaa aacacttctt acaatcaatc tttagctcct caaataactt attatcatat
     3781 atatttttat gtatatctat catatttttt ctcctcttaa gctttatttt taatattata
     3841 ttatctataa ctaaatgata atcaagtccc ttatttaaca gttcttattc ttaattataa
     3901 tttaaggtat tatttattcc aatagcatac ataagtttct ttatacttgt aacaatacct
     3961 ttatactaag acttatttta aatgtagcac tgtaataata aattgtttat atactcataa
     4021 tgtatttgat aaattgtaaa tctttataga agttgaaatt tttaatgatt ttagaatcaa
     4081 gatataataa taaatacaat ttataaaatc agaaatatat aaattaacta tacaaaccta
     4141 aataacatag catttaaatt cacaatttca gtataaatag tacaaagtat attaatttaa
     4201 ataagattgg cttatttgta gaggagagaa tattttggtg ttcaataaaa tttatattta
     4261 ataaaaccat aagaaaatat aaagataaat aaacaaactt ttattactta atagattctt
     4321 atggttttat actgtactga aaaacaataa acactacagc ttatttataa tattacgctt
     4381 ttttaacaac agaagacttt agcttcatag ctccaaaacc atctatctta cattctatat
     4441 catgtccatc tgatgagtca tggattaaac ggatattttt cacttttgtt ccaatcttta
     4501 tagatgatga acttcctttt actttaaggt ctctaattac tgtaacagaa tctccatcat
     4561 ttaatacatt tccatttaca tctttgataa tatttgaatc ttcttcagct cccaatccta
     4621 atgcccactc atgagagcat tctggacaaa ctaaaagagt tccatcttca taagtatatt
     4681 ctgaattaca tttcgggcaa tttggtaaat tttccatctt tatatgtcct ccatttgtta
     4741 tttttaaatt tcttattata taaaatttct tttgagcctt tatgatatca tattatactt
     4801 atatttacaa actactaatt tacttaatgt ttaaattcta ttcgtattat tattaaactt
     4861 gatttagctg taatctcttt cttatattta ttatagttga tattattaca attttataac
     4921 tctatctgcc tgctccgata actctaaatc atgagttaca aatacgatag ttttaccatt
     4981 cttatttaga tttttaagta tatcaaatac tatgtctcta ttttttttat ctaaattacc
     5041 tgtaggttca tctgcaaata ttacttcaca ttcttttaat attactcttg ctaatgctac
     5101 cctttgttgt tctcctccac tcaattcgta tatctttttt ttattgtatt ttctaagacc
     5161 tacagactca agtgcgtaat ttattttttc attatggttg atattttttt gatactttaa
     5221 agcaattttc aagttatttt caacattttc attctcaatt aaagcataat tttgaaatag
     5281 atagccataa aaatcccttt gtaaaattct tatctgttta ctatttggat tttttatatt
     5341 atttattata atatcaccag aattaggttt ttctaataat cccattatat taagtaaagt
     5401 tgtcttacct gaaccactat ttcctgttat cgctacaaac tcgcccttag aaatttctag
     5461 gttaaatttg tctattatag atttatcttt aaatttttta gatatattta ttaatgaaat
     5521 catcttttta atgttctcct tttataattt tattaaatga actatttgag ataatcttgt
     5581 ctaatacaat agatacaatt acttctataa ctaatatcag aagtccaaat aagataatat
     5641 tattacttaa atctactctt gttgatataa tgcctattgg aataatgttt gttaatatta
     5701 atgagactat taacaacata cttctagctg tagttgaata accaaatatt tttttaatat
     5761 ataacttata tttatttctt tcatagtagc tagctactat attgtaggta atcattaagt
     5821 ttgatattgc tattattatt atagtaaaaa tttcactatt caaatatttt tctagtttat
     5881 ttatttctaa accatactca ttataaacag catgtaaact ttggatatgc gcttctacac
     5941 cttgagcttc aataatgtta cttatatctg caagggcatc taatttctta gaattaaaat
     6001 atacacatct agatatataa gaaagataaa aagaatcatc aatattatct atgtcaatta
     6061 ttgctattgg gtcatcaatg agattcctat tgtcatccat aacaagggag ttataagtaa
     6121 aatagctttg attattatct acatatatta tattaacatt taaatctgac ttctttgttt
     6181 tatttaactc tctatttaac tctttattgt acatattttc tacttctatt ttttcaaaat
     6241 aaaataattc tctaaactct tttttaatct cattttcatg tacttgtaac tttttaggaa
     6301 ctaataaatt tatagtctta tcgtcataat cgattagatt aaatatttct ttgccatttg
     6361 agtaaatagg gttaacttta agatagtttt tatttatttt tatattttta ccatattgac
     6421 ttacttctgg gtttttaccc tttgaattta tttcatacat gtaacttcca tcttcaagtt
     6481 tttcaaaatt agttgaatct attaagaaag catctttttc ttctaccaaa tttttataga
     6541 aacctttaat cttggcattt ttaattactt cttcctttcc taaagattgt tcgcctgtat
     6601 ctttaagagt aagattataa acattttttg ctttatccca ctcagataga tttcctaact
     6661 gagtttgtag catattataa tttaaaatac agttatttat ggataatata agaaatgata
     6721 caaatacaat tttcaatgtg aaatgcatta tatttacaaa accataagat ttttttccat
     6781 ttagagatag cacatattta gaacttctac tattaaaacc aactattaat atagatatca
     6841 aaatatttat caaaatacat ataatcgaaa acatagcaaa gtataagcat gctctaacac
     6901 ctatatttaa ttttaaaata tagattatat atattaataa tgctgtgaca gatgataaaa
     6961 tcataattct aaatatattt tttataaccc tagcaattat atctagctta gagtatccat
     7021 ttaatcttaa aatagaagcc tctctactca cactaattga ataatgtatt attgtagcca
     7081 agaaacaaag gataattagt attaaatctc taattattga aggatttaag tataaatttg
     7141 agttaacata tctatcatgt aatcttgcta caaaaatact tctgtctaca tttagttctc
     7201 ttaatatttt atttattgta tcttcatttt gagttgaaat atataagatt cctgattctc
     7261 caacttcact tattttacca aaatccctaa ttaaaattga tatgtcttta ctcatagttg
     7321 agaatttacc tacttgatta ctattattac tattattagt gtttatatta ctaataaatt
     7381 catttgaatt agtattagga aattttcctg attttaactt tatattgtta ttcaaacttg
     7441 tatcagtcgt atatatagat aatttttcat tattattaaa aacatacttt gatatagcca
     7501 cattattttc atctgcaata tctttaatcc acttactata atttttattt aactttttat
     7561 aatctttaaa cattacagat atacttgttg tatcgttata taaaatatta ttaatttcat
     7621 tatctttaac tatatttaga cctaataaag atactattaa tagttcaatt gtaaataata
     7681 catatattat ctttttcaat tatagctccc tcctaattgt ttttattcta aaagcaaaat
     7741 atattttact ttagttaaag tataatgctt tgtacctttt ctaaacaact actatattgt
     7801 cacattctaa atggcaagtg tctatttcgt cacaaagata aaaataacct taaaacagtt
     7861 aattttttat aatcaactgt tttaaggtta ttttttattt ttagcaaaca atattttaat
     7921 agtggtacaa aattagtata attcattaat atagattcta gtatttttag caatcaacta
     7981 aaataatatc ttatttaaaa caaaatttaa tttaagttca atataaactt tacctcaact
     8041 agaaaatatt tcttctaact taggaattaa tgtgtggata tctcgtcaat agaaaactaa
     8101 ttcatcctcc atgttaaatt tctcttatat ttttaatgat ttcacgaatc tttttggcat
     8161 tttcagtaat atcctcaata gttcctttag ttaataaacc accaaatcca catacatcaa
     8221 ctccattttc taaccatata tgtaggtttt caagattaat tcctccactt gctaaaactg
     8281 gcatatcagg tattggtgtc ttaaatacct ttactagttt aggtccatag aaatctgata
     8341 ttgggaaagc ttttataaaa gtcgctccta aacttagtgc atctactgcc tcagtcaatg
     8401 atgtacatcc tggagcataa ggaatctgat atctattgca aatcttcgct acaccttcat
     8461 tgtaatttgg tgcaataata aattgagctc catgtaatat agcatgacga gctgtttcac
     8521 tatccaaaac tgttcctgct cccacacaaa gtatttcacc atacttttta ttaagccctt
     8581 gaattatttc tccagcatta tttagggtat aactcatttc tataactgtt ataccacctt
     8641 ttatacatcc ttcagcaatt tcacatgcac ggttaagatt ttcttctctt acaattgcaa
     8701 gagccccaca ttctgacatt cttttggtta tattcatctt tttcatatta tcacctctat
     8761 actcatcaaa ccactttact tattcattaa ttttagtcat gttttataat atatatatta
     8821 attatatacc agtgtaattt ccctatttat attatctatt ctcactactt ttattaagta
     8881 ttgtcctata attttcaact actcgtttaa tttcttcaag tacttcccca gttggttcag
     8941 ttacaggaag tcgtgcagtt ccaacattaa ttccattcaa gacaatagtt ttcttcataa
     9001 cagatggaat tgttccaagt tttagtatac gtcgaagttc ttctatacta ctttgagctt
     9061 tttgagcttc ttccaaatcc cctttcataa agttttcata gatagaaata ccaatctctg
     9121 tgaggaagtt tgttgttgct gcaacagctc cttgactacc agcttttaga ctatccagaa
     9181 taagtgaatc tgagcctgaa aacacactaa aattctcatt ctgcgttact tcaatataag
     9241 ctttcatgtt gtcaagttta ccactactat ccttaattcc aacaatattc tttatctttg
     9301 ataattcacg tactgactct ggttcaatat taattccagt cttacctggc atattataca
     9361 ttataatagg taaattaact gaattagcaa tagttttgta atgtaagatt aattcctgtt
     9421 gtgttggtgt aacaaagtat ggtgtaataa tagataatgc acttgctcca atcgcttcca
     9481 ttctcttgga taaatctata acttcacgag ttgaattacc acctgttcca acaaaaacag
     9541 gtacacgatt atttgtatga ttaataacaa tctttgcaaa ttcaactttt tcatcatctg
     9601 ttaacacatg acattcacca ttagtaccca aaataaataa tccataaatc cccttttcaa
     9661 tcaaataatc aattaattta catgttgctt gtgaattaat attttgcttt tcatcaaatg
     9721 gtgttatcat tgcacatata atccctttat atttcataaa attatattcc ctctctaata
     9781 gactttccaa gacttcctgc tggatgccat ttcaaatact gctgtgtatt aagaccttta
     9841 gcatattgta atacaacact taatgattgt aatactattg tttcagctaa aattgaagca
     9901 cgaggagctt tattaagaca atctccttcc tgtttaacgc ctgcgaacag gaatatatca
     9961 gaaatatttt ttaaaaatga actctcatta ccacttacac caattatttt tgctccattt
    10021 acttttagag tcttaagagt tgcttttaac tcttgagttt caccactatt tgatattgca
    10081 ataacaacat ctccttcaac tacttgacca cttgacccat gtactgcttc tgtaccatgc
    10141 aatatatatg ctgatgttcc agtagatgaa agcaatgatg aaatatatcc tgatacatgt
    10201 cctggtttac caataccagt cacatgtaca cgtccgtgat ttttttcagc ttcaagaatt
    10261 aattcgcatg ctttatttat agatgtctca tctattgagg caataaattc tgacaattca
    10321 ttagatatat tatctaaaaa ttcagataat gtattttcca taagatgtat cctccttgaa
    10381 atcatagtaa ctatgactat acttttatat aatttagttt aaataggttt tataaattgt
    10441 aaaatattat gtaatatcga accaactacg ttgtaatcaa tattagggaa agttgctcct
    10501 tctataccaa gagtcccata aactggatat agtataactg gtaaaaatgc aagtaataac
    10561 ccaacaacaa atgacccagc aatagcccct ctccatcctc ctgaagtatt gccaaatatt
    10621 gcagctgtac ctcctgagaa gaaacatatg tgagcagctg gaataattac tacaggaaat
    10681 atactaggaa atgatgccat aatagcaatt gctaatagac cagcagcata tgctgatata
    10741 aacccaataa taactgcatt tggtgcatat ggaaatactg ttggaacatc tagcgcagga
    10801 cgtgaacctg gaatatactt ttctgaaatg attacaaatg ctgcagttat ttcagctaag
    10861 aacatacgaa cacctgacat caatattgac atacctgcca caaatgtaaa tgcctctata
    10921 agcggaaaaa ccaaccaatg tgttgaacca gcaatttctt gagtaaatgc ttgaccagct
    10981 tttaatgttg caatatagaa taagattaac ataactatgg atagacccat taagaagtct
    11041 ctgaatattg ataaccactt aggaaattct atagtctctg ttgtatcatc tttatgtttt
    11101 gaaaataaac gcccaatata acctgataaa ccataaccaa tcatgttaaa gtgaccaata
    11161 gcttgttcgt cgccacctgt taattcacgc ataaatggtt gacagaattg aggtagtgca
    11221 gcagacataa accctaagat agttccacct attaaaatag tgattgtatt atcaaatcca
    11281 tgtgctttca tgattaaaac taatacacaa gaaaagaata aactatgacc tgttgtaaag
    11341 aaaatattct taaactttgt aatacgtgca aatactaaat ttaatatgaa tccaagaatt
    11401 aatgtacatg aaactgcaaa accatatgta ccttgtgcaa gtgctgtagc tgcttctggt
    11461 gaagctataa caccagtaat tccaaagcca tgttggaaca gtttattgaa attttgaagt
    11521 gctcctgtca tagcagaggc accaatacca aatatcaaaa aaccgataag tgtttttgaa
    11581 gaccctgtaa atacttcaac ggctgatttc ttttgaagaa tcaatcctat acatgcaact
    11641 acgccaagca aaacagcagg tgtactcagc aaactaataa taaactccat aaatattcct
    11701 ccttatcata ttaaatttat ccctcttttg aagcaaggaa tttttctagt gctgtcttta
    11761 tttcattacg gtcaacaata ttgtgaatcc caattatatc aaacttcaaa tttttatttt
    11821 ttaattcttc agcaacatca attaatgttt ctacagcatc tccattgaat ccatttagtg
    11881 aatccaatga accatgttct acttcaattg gaaagttatt ttccttaata atttgttgag
    11941 tctttatttt taacattaaa cttgagccaa ctcctgctct acatgcgatt aaaattttat
    12001 acataattaa atcctccttt ataattcgtg tgtttttata aaatcaataa tttctttagc
    12061 ctctttagca ctatctaaaa ccttaaaaaa ctcatcttct tccaatagtt caactagctc
    12121 agacatagca cgtaaatggc tattattgtc tattgcactt agacaaaata catattttac
    12181 agggtcgttt tcgttattcc caaactcaat aggactatct aaagtagcaa tgcctattgc
    12241 aatctctttt gacccagctt ctggtctagc atgtggaagt gcaacatgtt ttgtaattac
    12301 tatgtatggt ccagatgctt ttgctgagtt aatcatagca tctacatacc cttcagttac
    12361 tttgttattt tcaactaata cagaagctga ctgtctaata gcttcctccc aattcttagc
    12421 ttttaccttt atttttacta gtgtttcatt agtaatctca cttagcatag gcccctctcc
    12481 ttccttagat gtaaaattct ctatctgtgt aaaataagct aatagctcac ttgaaagaat
    12541 actttcagag gttacatttg catatttttt tattatatcc ataacctcat ctattttagg
    12601 ttgtcttaaa taaatatcac ctaattgaat atacacttca cgtgtaatct tatatttttc
    12661 ttttgtggtc ataacagggc ttactttaat tactggtaca tctgttttta aaccctctgc
    12721 gttataatta gtagtaaata cgatatctac aggttcactt atattttcta atccaactga
    12781 ttccgacatt ggtataaagt gtaagtcagg aaataagttt gtaagttctg catgtaaaat
    12841 agctgaacta ccaacgccat ttgaacaaac tattaatgct attctttttc taaaaccatc
    12901 aaattctttt ttatttgaaa aaagtgttgc aaagtgcatt gtaagatatg ctaactcttc
    12961 ttcctgaatt tcttctccaa ataatccgca aaactctttc attgcattac ttacaattct
    13021 ataaactaat ttatattctt cttttacttt ttcacaaatc ggattataga taggtaattt
    13081 aaataaaagt ctatagtagg caggtctaaa atgagaatat aattgtttaa atattttttc
    13141 ttggcttata tagtaaactc cgcttaaata tccaaacttt gtcatcattt tatttacaat
    13201 ttttgaaatt acatccttat cttctgtatc ttcttcataa tttcctaccg atatacctaa
    13261 aatccaagca ctaatataca ttatattata aggctttatc ttatcctcac tcccatatac
    13321 ttctaataat tccttagcaa atttatactc cttcattgaa ctcataactg aaatattagg
    13381 aatatctaca ttttcctgag taactattct agttgaatac atccttgctt ttagaaagat
    13441 aaatatatat ataaattcta ttaatctatc ttcaacaaaa gtaatttcat atttttttat
    13501 tagctttaat ataatttttt tagcctcttc aaaactatct aaattatatt ctttaataaa
    13561 aatatcaaat actcttgaac ttttctcatt agacaaagtt tctataatgt ttttaataag
    13621 cactctccta atttccatct cattgccaac tatgtaatat ccatttatac gattgttctt
    13681 aactttaatg ttcttctttt caagctctgg tatcaaatcc ttgaaatcaa gatttacagt
    13741 agacctactt acttttatag aatttataaa atgattaatt gaaatatcct ccatgttaat
    13801 aaaaagcatt aaatacatat ataaaagtct ttctgtctta ctaaaataat aagtattctt
    13861 agaatagttt ttttctaaaa tttctttaat tgcttttttt gtttcttttc taacgataat
    13921 atctttatca gctcttaaac atataagtgg tacatttttt actttaagca tgtcattgat
    13981 tttatttatt ctataagtaa tttgtctctt tgatgaatta gactcctctt gtaatccatt
    14041 agttgtaatc aatgtgctat ttaataaaat ctggagaatt gatacaattt ctttgtccac
    14101 taccattaac ccctactttc ttattaagat tagtcttcat attatcattg gattctttca
    14161 tcacctccac aatacttatt ttatttgaga attttatcgt tttcaatttc atttaagtat
    14221 aacttttgtt ttagattgag acaaaaatta aacttatcta aaatagctac tatattacac
    14281 ttatagattt tataaataaa aaagggttcc ttatgtaaag aaaccaaaaa aaatttattt
    14341 aaagaatcaa agcgatacta attatgcata aattaccata aataaaaggc acattaattt
    14401 aaaataaaat taatgtgcct tatttataat tctaatatat taaatttatt ttttaacttg
    14461 tagtttaata tcctttttct tcaattaatt catataaata aagtatttaa taataaatgt
    14521 tccttatgac tttaatattt taaaccaaga aagttaaagt ttctctcata taataaattg
    14581 acacttatct atttcattct tttaagtgta caataagtct tcttctagca gtaagagtaa
    14641 gtacttcctc tacttatcac aaactataat ttttatctca ttggtatata tccacgatac
    14701 tgtgagtatg catccatagt tgctatatca cctaagaata ctgatgcgat attgaattgt
    14761 ggttttaaat atgggaactc ctcttcttgt gcccattctg gttttttaat aaacgacttt
    14821 gtctcttcaa ccacagcttt ataccattcc tcttcccctt caaaccatag ttctgataca
    14881 cggtcaaaag cagttgctcc ataattaatc attattttac tactgacaaa acgattaaca
    14941 tagcaacagt ttgtaaagta tggcactacc tcatcataga accatttttc tccttcttct
    15001 ttagagatac catctggata cttaatcata aattgccaac gatagtttgg tccatcttgt
    15061 acagtacgtc cttttcctct aaagtcttct tcccagttta ttggaacaaa agcaaatata
    15121 aatggtgggc atccattatc tccacctact gcacgtcctg catctccact ttctgcattt
    15181 tcatgaaccc ttttattttc tacatctggt atacaccctt gccaacgtag aacatccata
    15241 ggcatatatt ctgtcataat tctatttgcc atctcaggca tatgttcatc tacaagccaa
    15301 taatgttctg ttagttgcat cttacgtgca ccaaaacgct caccttcatt tggtgtagga
    15361 tatgcttgat aaaaggcata tttagtacaa tatggtccaa aagtgctaat actatctgga
    15421 atatggtgac gatataacca gtccattaac ttaggtctat attcttcctt ggcaagtcct
    15481 acatatatta aacttctcat tattttaaat tcaaaaccca tagccattct cctataatca
    15541 aagtatattc acatgctttc aatttccctt tttcttatct ttactcaaaa tatatattgt
    15601 aaacaaatct acacctgcac ccacgtacca tactcctact gccatagcta cagcaatttg
    15661 aatacaaata tcttgtaaat taataatatt ttcacctgtc ataatagtta gacctatagc
    15721 ccaaccacaa agccatgatg gtaggaaaat tattttttca aataaggatg caattacaac
    15781 tgcaaaaaaa cctaatatac taagtgttaa aaataattct aggttttgat tgaaattcat
    15841 atatcccata attaaaatgg ctaacactgc ccataaatct ccacaccaaa aaccaaacat
    15901 tatttttaat ccatcttttt gtttattacc attagttaca tataatcctg cacagattag
    15961 tgctacagct ccagttttta ttcctatata aggtgctaat actgcccata aaggaggtag
    16021 taaagcaatc cccatagaca gtgttaatat ttcttttttc tttctatcca tttcctatat
    16081 tgtaattagt agcttcccta gcttgcctta tctccaatag cttaattgct atttgattta
    16141 caatcataga tactgcaaaa taacttaagt ataatggtat aaatataaca ataaatgctg
    16201 gagttaatcc aatcttgtaa tatgtaagta ccaaaaaaat aactgctaaa ataatttcaa
    16261 tttgtttaaa acttggcttt tcattcatac aataagcctc ctataacatc taacatccct
    16321 taaaaaagag tcttagtatt ttaattatta tcctaatgaa actccagctt gcttttcttt
    16381 agttaatggc tctataaaat tataagatgt ttttctttct ttaaattctt gtgttttacg
    16441 aacttctgca taatgacctt ccattttctt aatatcatca ccatgtataa acttacctgg
    16501 taaagtatca tgagcatgac ctaattgctc ttctggaaaa ctaaatatag tttttccact
    16561 tatagtatct aactctccaa ctataccaca tagagcacaa attgcctttt tagtacctga
    16621 ctcaaggtag aataatctat tgtggcaatg tggacataca ccttcttctc cttgatatga
    16681 agctttttca aaatcttttg ctgcttcaac tatatttaaa ccaatttgat gaacttgaac
    16741 aagccttgta tcatccatta ctaattcctt tgcccatggg aaccaagcat tatctataac
    16801 tttccacatt ggtatgagag ctagcatagc atgttcacat tgaactcttg taccccagtc
    16861 agaacctcct attcccataa atgatattac tttatcatta agcattcttg agtctggaat
    16921 ttttcctcct atagcttctg caacttttgt tcctataatg ttatttcctc tatctgctct
    16981 gggtccaaaa cggtccatta tagtatggaa taatcctgat gccccttctt caaaaatagg
    17041 gtctgaaact actataccat ctgcatcttt cattttgtca agcaaccatt caaaatcatc
    17101 ttttaataca cacatactac ctttcccgga taataaagtt ttaacgcacg cacaacatcc
    17161 agtacaatgt ttaatgttaa tagatgacat ttgaataaat tctacttcag cccctgcttc
    17221 tttggcacct ttaagtgcaa ctttacacat tgtgtcatta tttccatttt ttgttccaaa
    17281 agaaatacct agtattttca ttttcaactc tccctcttta gtatttattt aatcttcttt
    17341 catttttaat atatagaatc catcatctaa agcatctatg atgatgtagc ctctatcatc
    17401 aacgataaga tcttctgtta ctgccaagcg tggtcctggg aatcctggga aataagattc
    17461 ttctggtgtt ttatttggat ttggtggtat aaaatatgct agctctttga tataatatgg
    17521 gtctgataca tcataaaccc ttagccctgc atggaaataa cagcaatata ctctatctcc
    17581 tctttgctct aaccatggct tattatccat tggttcatga agattatgtg gaccaaatgg
    17641 ccctgctttt cctaatcctg caacattaaa gttaggataa gggaaatctt ttggaacttc
    17701 aggatatggg aattgagcaa ttaatgttgg ttttgttggg tcactaacat caaccatatg
    17761 tatattattc atagcttgaa catctgtaat gttatctggt ttaaagaact ggaatctttc
    17821 tccctcattt tgaacaacga ctaaatctct tcctggcaat ggtaatgctg tatgagttct
    17881 tgcacctgca agtctactag aaaatgcagg cataaatggc aattcaccta agcatcttgg
    17941 tcttgttaaa tcttcaacat ctaatacaac taaaccagct cctccataac cacaatatgc
    18001 ttttccgtct cttacaaatg gaggtccatg aagccatcct ttatccataa attctgggca
    18061 atgaggtgct cctgcatcaa aagttctatt tggatatcca tctgcatatt ggtctggtct
    18121 ccaccattta cctatttcca ctggattatt aggatttatt atatctatga ccctataaat
    18181 cagaccttca aagccaacac aatcacttga taaatgtaca tatcttccac cattgtacat
    18241 gaatctatga acacccatta catgctttaa gccacaatcc caatatccta aaaacttagg
    18301 atttaaaggg tcttctttta aactgtatat tctaattcct gcttcacact taatatttgc
    18361 taatttagct tggtcaacaa gtgctcctgg tccacttcca caactcattg ctactatcat
    18421 taaatcatct gctatttgaa tttttggagt tgttgttgta ggatactctt tagggtctaa
    18481 catttgaaaa tgttttataa attgtggatt ataagggtct gtaatatcgc taatcattac
    18541 tccatttttt ttatttcctc caaaacagca tccatataaa tagtattttt catctctttt
    18601 gtacagagcc atttgaaaca ttccacaagt tccatctaag ttattaaagg aacaaacttc
    18661 aagattttta atatatccgt tatttccgtt gccttttgca taaaatttat cttccatttt
    18721 ttctcccttc ttatttaaag tattatcaat tgatatactt tcattttatg agacaaactt
    18781 acttaaatcc aatatcgttt tcattggaat atataagtta ttcttattaa taaatttata
    18841 aaatctaatg taaagatata cttaaaacat atatttttat acaaatattt tctaatattc
    18901 tttaaaagta aattttctta tactataata agctttaatt atagataaat aaggtttaca
    18961 aacaatcaag tttaaatagg ggtgattatt tgaatactaa acaattagaa tattttatat
    19021 ctgtagcaga aaatctaagt tttaccaaaa cagcagaaaa attttatatt tctcaaactg
    19081 ctgttacaca gcaaataaaa gctttagaag aacaaataaa tgtaactcta tttacaagaa
    19141 gtaaacgtca tgttgaacta acaccagccg gaaaagtttt tttatctgag gcacgtacta
    19201 taataaaaaa tatcaatgat gctattgcaa aaacacaaca atttgcacat ggtttttttg
    19261 gaactttgag cattggaacc ttaataggat atgaaaaaaa caaacttcaa caatacttaa
    19321 aagaattttc taatacatac cctaatatat ctatggatat ttgtactaat gaaataaccg
    19381 aacttttaaa cttagtaaaa aataattcta tggacctcgc ttttgttatt aatccagaaa
    19441 atcaaccatt aaaagatttt gaatataaaa ctgtagaaag atattctttg gtagctcttt
    19501 tacctcctgg tcatccactt tacaatgagg attctatcga tttaattgaa ttacaacatg
    19561 ataaatttat ttttgtaaaa gaaacagggg atgagtatgg acaaaagtca atgatacaaa
    19621 atagatatcg tgaagcgggg tttgtaccta atgtggtaca acgttcaaat aatcttaata
    19681 ctattgaatc attggtatct tctaacatgg gaatatccat tttaccttca ttttgtgtta
    19741 cagatacaat gatagaacgt gatattgcaa tagtacctat aaaagaagta gaaaatagaa
    19801 ttgaagttgt ggttgtatgg aataaaaaaa atacaaatcc agcattagaa aagttcattt
    19861 ctataatata aaattaatca atattaacta taaaaatagc attaaccatt tttatatggt
    19921 taatgctatt ttctactatt ttattatcta acttttaatt taatatttat aatcgttaaa
    19981 aataactctg tttaacaaat ataagcaagt caaaagtgtg tctaaaaaag ataaaaattt
    20041 tattatatac aaatgtatta tctcaatata tctatttttt cattgttcat cagtctaagt
    20101 acatgacctg tgcttactaa agaattatcc ccataagttg tatgagctaa caccgaacta
    20161 gcaaccccaa actcaactat atcttcactc ttcatatttt ccaaatatga atatataatt
    20221 cctgccacat atccatcccc agcaccaact ctgtcatata tttctaactt atactcccta
    20281 ctttcaaaat aaccatcatc atttgatata tatcccttta taaatctatt attcatatca
    20341 tcaaatcttc tcatagttcc agctacaata tctatatcat actctttttt aatcaattct
    20401 gaactagatt ttatatttag actatctata ttaagtatct tatttatatc tctataacta
    20461 gcaaacagcc catgacaata tggtagtatt ttcttataat catttactaa atcatcataa
    20521 gtcctttctg gatttaatga ttgtctaaaa ttaaaatcaa agaatacttt tatccccttt
    20581 tcataagatt ttctagctag ttctaaagca atctttttaa ctgaatctgt aagttgcata
    20641 gcaataccac aaatatggac tatatccatt tcttctaaac atttatcaat atcataatct
    20701 tgtaatttac tctcacaaaa tgaactattt aacctattca aataagtcac ttcagaaggt
    20761 ctttctccat acccattttc aagaaaatac actcctatat gattccctcc atatgtgata
    20821 aagtcatctt ttactccaag ctttcttata tttgcacttg atgctttacc tacattatta
    20881 ttaggcaact ttgtcaacat ataggtattg tatccaaagt ttttaagtcc agataatata
    20941 tttaatcccg tgcctgtaat tatataattt agttcattgg tttgtgttaa gaacttgtag
    21001 tcaggtactg aaagtctcat cattatttca ccaaaagcta atatattcat aaatctatcc
    21061 caataattct ttcattattt tataaagtac tcctatatct tccactttcg tatcacctgt
    21121 ttctttatca attattgatg tataaacatg aggtataact ttttctacac cagcatcaag
    21181 tgcaatttga actatctctt tgaagttatc taagtctata ccccctgttg gttctaaata
    21241 aaaattatgc tttgcacaag cttttgcaac ttctatatat tcatcctttg ttttaagccc
    21301 tcccattgga aaaaacttaa ttgagcttcc tcccatatct ttcatcatag ctattgctgt
    21361 ttctatagga actataccat ctttttcttt tgaacttaat ggacctgtgg atatttttac
    21421 aaatcctggt ttaccagttg gagaaactaa tccatttatt atactatcat tttgaccaag
    21481 caatgctcta gatgtagcga cacctgtaaa cacttgattt atatgttgtg gctgaagttc
    21541 tttagaaatc tgagatacca tatttgactg atttgggtct ccagctccaa gacccacaga
    21601 tattgcatta tctatttcct ttgagtattt tttcatatct tcaatagctg cttctacagt
    21661 gctataattc tttgatagta ctcctactag tacatgtcca tcacaggcat catatacttc
    21721 tttagcattt tttatgcttc ctgcaagaac atttaaacaa actctatttt tataatattg
    21781 tggtatacaa ttcatattta tcccctccat tggtaaaatt caatttatta aatcattata
    21841 atgtcactta ctctacactc taactttcta ctatattttt tatcctatta taaatattat
    21901 ctaaatcact tccagttaca cttcttatat caatctcaat cttgccttca ttagccttat
    21961 agtctctagt gtatatagct atctctccac ttctaagttc atttataatt tgttgtgttg
    22021 acttatttat tatatcttca ttaaagttaa tttctccacg aagtatttct ctacctgctg
    22081 aatctctagt tatactagca ctcactccct ttatagtatt tatcttgtct ataaatggat
    22141 ttagcttgtc ctccatttct tttaaagtta ctttatcatt acttatatat ctctcaattg
    22201 cacatgttag acctacaata ttttcttttc ctattttcat agctcttcct ataccctttg
    22261 attgaagctt tacattatct atgtattttt tcttacctat tactaacccc gagctaggac
    22321 cttctatagc cttagcacca ctgtatatca ctaggtctgc tcccaattca taatatacat
    22381 ttaaatcttc ttctgctgct gcatctacta tcaaaggtaa attatattct ttagcaactt
    22441 gtactgcatc taatacactt agcatactct tttgaacaca atgatgagat ttaatataca
    22501 taattgctgc tgtattttca tttatctctc ttattatatg ttccttgtta cacatattag
    22561 catagccggc ctcaacaact tctcctcctc caagtcttat cattacttct actggaactc
    22621 cataatcaac attatgcccc tttggaatta ttatttccct tttaacttgt ttcttaggat
    22681 tatttacatt gtaaactaga ctcatatcat cttttgctat cattgctccc acactctgtg
    22741 caatcccagc agatgcacta gaaactatta gtgcactttc acaccctaat aaatttgcta
    22801 tatattcacc tgttttattt acaagttctt ctattataag gaaattttta cacccttcac
    22861 ctattgcttc aataacttcc ttatctactg ttgatactcc tagtatagtc attttgcctg
    22921 aagcatttat aacacgtctt acagagtatt tttcaaatat actactcata caatagtttc
    22981 tcccttctac tcattctctt ctatagagtt gcctatattt aaatattctc cattgactac
    23041 tactgctctt ggctttatag acgtacttgt gattactgac ttattcaaag aatcttgaag
    23101 ttctttttct tcattaacca catcaaatat agttaaatca gcatcatatc cttcttttat
    23161 taaaccttta ttttttaaac ttattgcatc tgctgcattt ttagtaactt tatctattat
    23221 atcttcaagt gaatagccca tatatataaa cttttccatt gttgtagata gattatacac
    23281 tggaccattt attctatttt taatatatat atctgtactt atagtgtctg gaaaaattcc
    23341 tgcatcctta gatttcattg caatatccat actaaaactt tcggttccat gacctacatc
    23401 aagaattata cctctttctt tagcttctat gatttccttt ttaacctcat tgcctctaag
    23461 tattccattt ggctttccat tatatatatg ggttaatata tctccttttt ccatataatc
    23521 aaatatatcc tctatcgttg gaggagaact cccaaagtga accatcatag gtagattaag
    23581 ttcatttttt attcttttag ctacttttaa aggttcaaca tcatttgaaa ttacaacact
    23641 tttactcatt ctagctttga ttccaacaac aaaatctttg tattttctaa ctgcattttt
    23701 aagttcatat tcatcaatat tcatcatatt tgaaagttca tcttgagatg ttattccctg
    23761 cttagcaata tttattaaag catatacatt ggtcttatac tttttagtat cttcataaaa
    23821 ttcacctata tctaaagctc ctgttgttcc tgcatctact acagtagtaa ctccactttt
    23881 tattccaacc tcatctggat aatccttata aaggtctagt ctttcgtagc agtgtgtatg
    23941 tatatctatc caacccgcac taataaattt ctctccatct aagtttataa tttgttttgc
    24001 ctgatactta gaatcatcta tatgattttt tacttctatt attttatcgt ctaatatagc
    24061 tatatcaatt atacttttat ctactgtttt tccacatttt attaatatgt ctatcttcac
    24121 tttactctcc ctctaaaata atcttaaatt tactccaagg ttcaataaag tctaaaagta
    24181 ttatttcgtc ttcctttatt ctacccacct tattttttct ttcatctgta caatcttgaa
    24241 gtatcaactg taattcccct ttatactttc caaagtcatc attaccaatc actacatctc
    24301 ctcttttaaa gatgttctta ttatcatgtt tagggaaatt ttctttagca tatttttttc
    24361 ttacttctgt agatcttgct acctgattag ttatatctcc tcttctaaag tgttgctcat
    24421 ttaccattat tatcttttct acttcagaag ttttttcttt agctactaca ttaaatacaa
    24481 cttggtattc atttacttca gacattcttt ttaattcatc ttcacttgca tatgcatttc
    24541 ctacaattac atcatcaatc aatcctgttg cgaataagtg cttagttgct gtagcaatat
    24601 ctaagtttct atgcatttca agcgtacaca gtccatcatt tatattccaa ggtccgattg
    24661 ttgcgtattt tgaattgaca aaagcagcag ttcttattcc atacttttta aatctatttg
    24721 agcatcttac aaaaaaatca tatggcaagc ctgtacccct ttgaggataa aaattgtgac
    24781 aaccatatat attatttaca tttggcttgt aagtaattat attatccaag tatgctacat
    24841 cattactcat atttagttcc acttttaaat catatggatt gaaagaaatc atagcttctt
    24901 tacttccatc atatccatag tcaagcctta ttccactagc atttaactca ctaaagaatg
    24961 ttaaatcatc ataagatatt tgtaatatat caaatatgtt tggagctata tcaaggatta
    25021 cttcaaatcc taattctctt gcataggtta taatctcttt aaaatgtttt ttaacttctt
    25081 ccttaggtct tgtaattgaa agcatgcaca taaatattct tttaaatccg tattttgaaa
    25141 ccatttttat atatgcctta tcatcttcca tgttactatg ctctggatat actgatattc
    25201 ctaatcttct ttccatcttt ctcctcctca aattacatta tttaaaatat ataaaattaa
    25261 aggcaaaggt cattcctcct accttcaccc ttaataactt ataagctttt tctatatttt
    25321 atatagtaca aaacctctat atctatgtat ttttataaat tttcactcat ttcttcttgt
    25381 tctctttgta attgttgttt ttcaaaaact ttgaagaatg gataataaac agcaaatgat
    25441 atcacaaagt tgattaatac taagatacct gctagtatac tccagttagt actcattact
    25501 gctgctattg gtgctggcat tgcaaatggt aatcttgcta ccatcatagg tataacccct
    25561 gtaatagtaa gaacatatga tagaattgtc attattatag gtccaattat aaatggtatc
    25621 cctagtattg gattcataac tattggtgct ccaaatatta taggttcatt tatgttgaat
    25681 aaccctggta aaaatcctaa tctacctaaa ctttttagat attgtgactt agaaaacata
    25741 aacattacaa ctaatgcaag agtagcccct gctcccccta tccatacata ccactgtaaa
    25801 aattgttctg taaatatgtt tggcattgca tgagctgata ctccttctgt gaatgcagtt
    25861 atattttctg ctatagacat gtcccatata ggtcttatta ctggccccat tatagctgga
    25921 ccatgtatcc caagtaccca gaataaagtt attaggaata ctgtcagtag cccaccacct
    25981 aaactatttc ctgataagaa tccttttaaa ggtgataaca tagcgcttag tgcaacactt
    26041 atatcaaatc ctaaaatatg tcttattacc cagaataaca ttaaaattac tgctgtcggt
    26101 aataatgcag taaatgaatt tgaaacctct actggaactc ctgctggcat cttaattgta
    26161 atattctttt ccttcataaa acgatatatt tcaactgtaa ttaatgatgt tacgatagct
    26221 ccaaataatg atggcgaatt aagttttgcc atatccatcc atctaccagc ttctataact
    26281 ccacctactg gttcaactac ttgagttgga actattgtag aaactaaaaa tgccataact
    26341 gcaagcatac caacacctgt tgtatctaaa tcgtagcttt tagcaagtga tgatgctatc
    26401 ccaaaagtag catatagaga taaaaatcct acagtatatc taaatggtat atctaatgtt
    26461 gctttaaatg gagctataaa atccatataa gcatcaatag gtatgtttaa taatattgtg
    26521 aaaaatgacc ccactattgt tagtggtaat gttgctatta tacctgctct tacagctctc
    26581 atatgtctct gattacttat cttcgctgct acaggcataa tcttgcgctc taaaaaactt
    26641 gcgaattttt ccatagttat ccccctgtat tattgatatt atcctttatt tataatgcaa
    26701 taacgatttc ataacataaa ctaattttta tgtaattgat aatatcactt atttattaaa
    26761 aatattataa cattataatg tttggttgta aacattataa tgttataata tttttcgttt
    26821 ttacaactaa acaaaaatcc tgagcatatt atcatttcaa taaactcagg attcttatta
    26881 tatttcgtat ttaattacgt agttttgtac ttctgtatta tatcttgcaa atgatagctc
    26941 aactatatta tcattctcat cataggtctt tctttttctt cctaataata tgtctttagc
    27001 tagatttaga gcctttgata cctctagttc tgattcttct ataaaaaatt catcttcaaa
    27061 tctagttata tttatatttc tcctatataa gtacatatat aaagaacttt cattattaaa
    27121 actttcaaca gttagatttt catcaaaagg aatataatga gtaaaaaata tatatggctt
    27181 actatctaag taatacattc tagtaatctt gctacatttt tctccaaata tactaaatac
    27241 ttgtgaattt gtatcattta ttattttttc aattgaaata gtctttttag ataacttatg
    27301 acctttagat aaaagtatac ttgaaaaaga ctctgctttt gatagcttat taaaaagact
    27361 attacttatt acttttgtcc cttttccact ttttttctct acataccctt ctgattctaa
    27421 tatctcaata gcttttctaa tagtaatttt actgactcca aattcttgct ctaattctct
    27481 ttctgttgga atattttcat caattttata ttcgttattt aatatttttt gctttaatat
    27541 atctacaact tgtatatata atggttgatt tcttaacatg attcacctct taaatta
//
LOCUS       DAMJVX010000032        27513 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMJVX010000032 DAMJVX010000000
VERSION     DAMJVX010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.034"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            85..1779
                     /locus_tag="PC407_001328"
     CDS             85..1779
                     /locus_tag="PC407_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDF2496783.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSHFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGNIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKISKKLGFEDFVEL
                     GYVRMMRSDYREDMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      2111..2221
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2325..2696
                     /locus_tag="PC407_001329"
     CDS             2325..2696
                     /locus_tag="PC407_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HDF2496784.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQDNKKTPKWTENVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            2792..3739
                     /gene="trxB"
                     /locus_tag="PC407_001330"
     CDS             2792..3739
                     /gene="trxB"
                     /locus_tag="PC407_001330"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2496785.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            3824..4141
                     /locus_tag="PC407_001331"
     CDS             3824..4141
                     /locus_tag="PC407_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HDF2496786.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            4410..5696
                     /locus_tag="PC407_001332"
     CDS             4410..5696
                     /locus_tag="PC407_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2496787.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            5808..6281
                     /locus_tag="PC407_001333"
     CDS             5808..6281
                     /locus_tag="PC407_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5940..5942,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HDF2496788.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            6313..7623
                     /gene="grdB"
                     /locus_tag="PC407_001334"
     CDS             6313..7623
                     /gene="grdB"
                     /locus_tag="PC407_001334"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:7360..7362,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2496789.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            7880..9412
                     /locus_tag="PC407_001335"
     CDS             7880..9412
                     /locus_tag="PC407_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HDF2496790.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNSDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            9423..10568
                     /locus_tag="PC407_001336"
     CDS             9423..10568
                     /locus_tag="PC407_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HDF2496791.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            10878..11957
                     /locus_tag="PC407_001337"
     CDS             10878..11957
                     /locus_tag="PC407_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2496792.1"
                     /translation="MSRVKNVVGLLGTKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            12070..12669
                     /locus_tag="PC407_001338"
     CDS             12070..12669
                     /locus_tag="PC407_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496793.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLPPKGQE
                     SSQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIAALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(12856..13728)
                     /locus_tag="PC407_001339"
     CDS             complement(12856..13728)
                     /locus_tag="PC407_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2496794.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            14145..15548
                     /locus_tag="PC407_001340"
     CDS             14145..15548
                     /locus_tag="PC407_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496795.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            15575..17119
                     /locus_tag="PC407_001341"
     CDS             15575..17119
                     /locus_tag="PC407_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2496796.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            17150..18541
                     /locus_tag="PC407_001342"
     CDS             17150..18541
                     /locus_tag="PC407_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDF2496797.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            18734..20230
                     /gene="abfD"
                     /locus_tag="PC407_001343"
     CDS             18734..20230
                     /gene="abfD"
                     /locus_tag="PC407_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HDF2496798.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            20365..20640
                     /locus_tag="PC407_001344"
     CDS             20365..20640
                     /locus_tag="PC407_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496799.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            20664..21971
                     /locus_tag="PC407_001345"
     CDS             20664..21971
                     /locus_tag="PC407_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2496800.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            21991..23109
                     /locus_tag="PC407_001346"
     CDS             21991..23109
                     /locus_tag="PC407_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2496801.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            23396..24316
                     /locus_tag="PC407_001347"
     CDS             23396..24316
                     /locus_tag="PC407_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDF2496802.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFIPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            24431..25588
                     /locus_tag="PC407_001348"
     CDS             24431..25588
                     /locus_tag="PC407_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2496803.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(25673..27172)
                     /locus_tag="PC407_001349"
     CDS             complement(25673..27172)
                     /locus_tag="PC407_001349"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDF2496804.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT        10730 a         3139 c         5176 g         8468 t
ORIGIN      
        1 ataatttata aaaaagtatt acatagtaca tgtaaatcta tatttataaa tacaataaag
       61 taaagttgta aaggagtgct ttaaatgaag ttttcagagt ttaagtatga aagacccaat
      121 tataatagta tgaaaaaaga gtttttgagt tgtgtagaag atataaataa ttctcgtaat
      181 tataaagagc aacaaaaaaa tatacataag attaatttat taagaaataa aatagaaacg
      241 ttgtcaaata tagcttcgat aagatatagt acagacacat ttaataaatt ctacaaagaa
      301 gaaaaaaatt attgggatga atatatgcct ttgtatgaag aattaaattc acatttttat
      361 aatgctattg taaattcaaa atttaaatat gatttgataa aagagtttgg agaacaattt
      421 ttcacaatag tagagtattc tttaaaaagt ttttctaaag aaataataag tgaattgcag
      481 gaagaaaata aattgtgttc agagtatact agacttttag cttcagctga gataatgttt
      541 gatgggaata taagaaattt atctggtatg gggaaattca tgtattctaa gaatagaaaa
      601 acaagagaat tagcaaacaa agcatattat aatttctttg aagaaaatga aactaaattt
      661 gatgacatat ttgataaatt ggttaaactt agagataaga tatctaaaaa actaggtttt
      721 gaagattttg tagagcttgg gtacgttaga atgatgagaa gtgactacag agaagatatg
      781 ataaaaaatg ttagaaaaca agtattaaaa tatgttgttc caatggccaa tgagctttat
      841 gaaaaacaag ctaaaagaat tggtctagaa tatttaagtt acatagatga aggcgtggaa
      901 tttttaacag gaaatgcaag tttaaaggga gattcaagat acataattca aaatggtaaa
      961 aggatgtatt ctgaactgtc aaaagaaaca aatgaatttt ttgattttat gttagaaaat
     1021 gaacttatgg atttagaaac gaaaaaagga aaaggagctg gagggtattg tacttatata
     1081 ccagattata agtcaccatt cattttttct aattttaatc aaactgctga tgatatagat
     1141 gtgttaacac atgaggcagg acatgcattt cagttgtata tgtctagatg gattgatatg
     1201 ccagaaatta attttccaac gcttgatagt tgtgaaatcc attcaatgag tatggaattt
     1261 ataacatggc cgtggatgga tttatttttt aaagaagata cagataagta caaattcact
     1321 cacttatcat cttcaattaa atttatacct tatggagtaa ttgttgatga atttcaacac
     1381 tatatttaca aaaatcctaa tgtagataag tcaaaacgaa aagaaatctg gagattttta
     1441 gagaaaaaat acttacctca tagaaaatat ggtgataatt cttttttaga gagaggttgt
     1501 tggtggttta aacaaggaca tatatttaaa aatccgtttt attatataga ttatgtatta
     1561 gctcaaattt gtgcgttaca attttggaaa aaaatgattc aagatagaga tgcaggatgg
     1621 aaagattata taaatatctg taaagttgga ggaacaaaat catttttaga tattgtgagt
     1681 atgggaaatt tgtattctcc atttgatgat ggctgtattg agtctattat aggtgatgtt
     1741 aaatcttggt ttgatgaaat aaatgacagc aaattataaa aaattatgag aattttattg
     1801 tataaaagtg aaaaattaaa aaatatataa aaagtgatta tttgttgtga aaatagtctt
     1861 gtttttaact aaattgataa aataaactaa aaaagatttg tacttaaaaa aaaaagaaaa
     1921 tacaagaaat atgcaatatc aacatttgga aaaaaaagta aaaatgtata caaaatacat
     1981 ataaaaaaac tattgcaaat tttatagaat gtttatatac ttaagtcaac agagatagaa
     2041 aaaagctatt tatagaaaac aaaggataaa ttgcttaatt ttaaattttc tgaaaaaaat
     2101 aataatccgg atgaagagta tgggagactg ctttataaaa agcgaccgaa gggacaagtt
     2161 taatcttact tacccaaaag attagatgaa attctcaggt aaaggtgact gtacttggac
     2221 ggggctctga aaatgatttt aacagggaag acaaattggg agatttgttt ttcctgtttt
     2281 ttatttatca agtaataaat aaaagtttga aagagagtaa aaaaatgata ataattacga
     2341 ataatccaaa agtaaaagaa gaagttcaag gcagagaagt tttgtttaaa gacacaactt
     2401 atattggaat cttagaagca agtagagatt tgatacatga aggttatgaa cttttatctc
     2461 atccactcta tgggagtgta aaaccaaatg aaacacctta caggactgtt atactaaaga
     2521 aaggaaatcg tttggatata aactctttaa ccttaattga agaagcaatc atcactgcaa
     2581 gtaaatttca ggataataaa aagactccaa aatggacaga aaatgtccaa gacgatttta
     2641 gagtaattga ttatgatata ttttataata caatacaaag aatgcagtat gaataaaaaa
     2701 atttgttaaa aagttattga aacagcctaa atattaaatc taatttaata ttaaataaaa
     2761 tgagaaattt aattggggag gataagtcaa aatggaaaat gtgtatgact tagtaataat
     2821 tggttcagga ccagctgggc ttgcagcagg tctttatgga gcaagagcaa agttaaaaac
     2881 tctaatccta gaaaaagaca agactggtgg acaaattgta ataacacatg aaattgcaaa
     2941 ttatccaggg tcagttccaa atgcaacagg accaagttta atagctagaa tggttgaaca
     3001 gtgtaaagag tttggagcag aaatgctaag agataacatt gttgatactg agctagatgg
     3061 agatataaaa gttttaaaag gggaaaaggc agaatataga gcaaaagcag taataatagg
     3121 aacaggagca acaccaagaa agattggttg tcctggagaa aaagaactta ctggtaaagg
     3181 ggtttcatat tgtgcaactt gtgatgcaga tttctttgaa gattttgaag tatttgttgt
     3241 aggtggagga gacagtgcat tagaagaagc aatgtattta actaaatttg ctagaaaagt
     3301 aacaattgta catagaagac aaggatttag atgtgctaag agtgttgaag aaaaagcaaa
     3361 agctaatcca aagatagagt ttttattaga tacagtaatt gaagaaatca aaggtgatgg
     3421 aatattagaa tcagttgtat ttaaaaataa agttactgga gaaactcatg aatactttgc
     3481 agatgaagaa gatggaacaa tgggagtatt tgtgttcgtt ggattagatg ctcaaactga
     3541 tttattcaaa ggtaaagttg atatggatga aaaaggttac ataataactg atgaagatat
     3601 gagaactaac ataccaggcg tatttgcagc aggagattgt agatctaaga ctctaagaca
     3661 ggttgtaact gcaactaatg atggcgcaat tgcatctata gtagctgaga aatacattga
     3721 tgaaaaattt gggaactaga atctgtaaat taagaaatag tgtctgtaaa tttaatgaat
     3781 aatatttaaa attaaaataa aaatttcaag ggggaataag aaaatgttag atttagataa
     3841 ggctacattt gaagaagaag ttttaaatgc agaaggtttt gtatttgttg atttttggag
     3901 tgaaggttgt gagccttgta aagctttaat gccagatgtt cataaattag ctgagactta
     3961 tggggataaa ataaaattct gtaagatgga tacaacaaaa gctagaagat tagctataaa
     4021 acaaaaagta ttaggtcttc caactatggc tatatacaaa gatggagaaa aagtagacga
     4081 agttactaag gatgatgcta cagttcctaa tatagaaaac atgattaaaa aatatcttta
     4141 atatgttgat aaagtattaa cattaaaaga aaaaatctat ttttatctga agataagaag
     4201 aaaaaggttt ttatgtccaa ctttgttaaa aaaataattt taaataaaat aattttttta
     4261 caataaatat tttttagtaa taaaataaag atttaaggca taaaaacaca ataatatatc
     4321 tttaaaaaaa taagataaat tgtaaaatga tataattttg agaaaaactc aaatgcatat
     4381 acacaaatta aataacagga ggtgcaagta tgcgtcttga attagggaaa atctttataa
     4441 aagatattca atttggtgat gtaacagaag taaaagatgg agtattattc atcaataagc
     4501 aagagatgct acaagaaatt ggtggagatg agcatattaa atctatagat atagaattag
     4561 ctcgtccagg tgaaagcgta agaataacac ctgtaaaaga tgttattgag ccaagagtaa
     4621 aagttgaagg taatggggga atcttccctg gtatcatgtc taaagttgat actgtaggag
     4681 aaggaaaaac tcatgcttta aaaggtgtag ctgttgtaac tactggaaaa atagtaggtt
     4741 tccaagaagg tataatagac atgactggag aaggagctaa gtatactcca ttttctaaat
     4801 taaataatgt agtagtaata gctgaaccta ttgatggttt aaaacaatac gctcatgaaa
     4861 aagctgttag aatgatagga ttcaaagcag caatgtactt aggagaagta gcaagaaatc
     4921 ttactccaga tgaagtttca gtatatgaaa ctaaacctct tcttgaatca ataaaagaat
     4981 atccagagct tccaaaagta ggatatgttt acatgcttca gactcaaggt ttattacatg
     5041 atacttatgt atatggagta gatgcaaaac aaatagtacc aactttatta tatccaacag
     5101 aattaatgga cggagcaatt gtaagtggta actgtgtttc tgcatgtgac aaaaacccaa
     5161 gttatgttca tataaacaat ggggttgttg aagacttata tgcacgacat ggtaaagata
     5221 taaactttgt aggtgtaatt ataactaatg aaaatgttta cctagcagat aaagaaagat
     5281 cttcaaactg gacttctaaa ttatgtaaat atttaggatt agatgcagtt atagtttcac
     5341 aagaaggttt tggaaatcct gatactgacc ttataatgaa ctgtaagaag atagaaatgc
     5401 aaggtgttaa aacagttata gttacagatg aatatgctgg acgtgatgga ggttctcaat
     5461 cacttgcaga tgcagatgta agagctgatg ctgttgtaac aggtggaaat gccaaccaag
     5521 ttgtagttct tcctaagtta gataaagtta ttggacattt agaagtagta gatgtaatag
     5581 ctggtggttc tgatggaagt ttaagagctg atggaactat tgaagttgaa atccaagcta
     5641 taacaggtgc aactaatgaa acaggatttg gtcatttaac tgctaaagga tattaagaag
     5701 tattttatta aacgattgag taatgttaat aaaacatatt atagataatt ataataagtg
     5761 ttatagatat ataaaaatat attagaaata taataaggag gattattatg agtttactta
     5821 gtaataaaaa ggttcttata ataggtgacc gtgatggtat accaggacct gcgatagaag
     5881 aatgtgtaaa aacagtagaa ggagcagagg ttgttttctc atctacagaa tgctttgtct
     5941 gaacagctgc tggggctatg gacttagaaa atcaaaacag agttaaagat gctgctgata
     6001 aattcggagc tgaaaatgtt gtgattttac taggtgctgc tgaagccgaa gctgcaggtc
     6061 ttgcagccga aacagtaact gctggagatc caactttcgc tggaccactt gctggagttg
     6121 ccttaggatt aagtgtttac cacgttgttg aggaaccaat aaaatcatta tttgatgaaa
     6181 gtgtatatga agaccaaata agtatgatgg aaatggtttt agaagttgaa gaaatagaag
     6241 aagaaatgtc tggtataaga gaagaatttt gtaaatttta atgcctaaaa gcaaaagggg
     6301 tgaaactaag agatgggaaa actaaaagca gtacattata ttaatcagtt ctttgctggt
     6361 ataggtggag aagaaaaagc tgatactaag ccacatgtgg cagaaacttt acctccaata
     6421 agtttacaac ttgataaatt attaggtgaa gatattgaaa tagttggaac agtagtttgt
     6481 ggagatagct atttcaatga aaatattgat agtgcaagtg aagaagtttt atcaatggta
     6541 aaaggttttg aaccacaact tttcatagct ggtcctgcat ttaatgctgg tagatatggg
     6601 gttgctgctg gaacaataac taaagttgta aaagatgctt tgaatatacc agctttaaca
     6661 ggtatgtatg ttgaaaatcc aggagcagat atgttcaaaa aggatgttta tgtagtagaa
     6721 acttctgatt ctgcagcagg tatgagaaag gcacttccta agatagctaa acttgcagta
     6781 aaacttgcta atggagaaga aattggaact cctaaagatg aaggatacat agctagagga
     6841 ataagagtta actacttcca tgaagacaga ggttctaaga gagctgttga catgttagtt
     6901 aagaaaataa aaggtgaacc atttgaaact gaatacccaa tgccaaactt tgatagagtt
     6961 gacccaagta aagctgtaaa agatttatca aaatgtaaaa tagctttagt tacttctggt
     7021 ggtatagttc caaaaggaaa tccagataga atagagtctt cttcagcttc taaatatgga
     7081 acttattcta tagcaggagt tatggattta acagaagaga cttatgaaac tgcacatggt
     7141 ggatatgacc cagtgtatgc aaatttagat gcagatagag tattacctgt tgatgttctt
     7201 agagatttag aaaaagaagg tgtaattgga aaattacatg agactttcta tacaactgta
     7261 ggtaatggta catctgttgc aaactctaaa aaatatgctt ctgaaattgg agcagcatta
     7321 gtggctgatg gtgtagatgc agtaatatta acttctacat gaggtacttg tactcgttgc
     7381 ggtgcaacga tggtaaaaga aatagaaaaa actggacttc cagtagttca tatgtgtaca
     7441 gtagtaccta tttcattaac agtaggggct aatagaatag ttccaactat agcaatacct
     7501 catccacttg gaaatccagc gcttgaccct acagaagaaa aagccctaag aagaggcctt
     7561 gtagaaaaag cattaaatgc tttaactaca gaagtagatg gtcaaacagt atttgaaaaa
     7621 taaaaataac acacacatat attatataga taaaaatata aacacacact aaaaataaaa
     7681 aataactata cacaaaaaaa ataatataga tttcatgaat ggataaattg gagttattca
     7741 ttcatgaaat cttaaaaaat gccaaaatac ataaacaata atagaatcaa atatacaata
     7801 aaatacaatg attagagctt ttaataagtt taataagatt aaaaaataag tgttcataaa
     7861 atttatggag gtgcacaaaa tgacttatcc agttttaaaa ggggcaggct atgtacttat
     7921 tcatactcca gatatgatag tgcaaaatgg aagtacttgt acagttgaaa gagcaacaaa
     7981 ttcagattca gaatttttaa aagaagtaag taatcatata agaagttatg aagatgtagt
     8041 taactacatg ccaaatcaag tttatatagg aaatagaaga ccagaagaat taagagattt
     8101 accaatgcca tggtgtgaac aaaagattga gggaactaga aatggtaagt ttggagaaat
     8161 aatgcctcaa gatgagttca tagctttaat gcaaataagt gatgcatttg atttagtaaa
     8221 attatcacaa gaatttattg atgaagtaaa gccaaaaatc gaaaataact accctgaaat
     8281 tgcaccattt gtaggtaaat taaagggtga tgatatagaa gaaggtaaag aattagtagc
     8341 aacacacata gctgaaggat tataccatga tggaaaattt gttggttatg taaaaagagc
     8401 acatgatgta gatgttaact taaatgccca tacaatgttt gaaaatctag tagtaaaagc
     8461 atctggagtt ttatctgcta ttcaaatgct aagacatagc aaaatagacc ctgctgaaat
     8521 agattatgtt attgagtgtt cagaagaagc ttgtggagat ataaaccaaa gaggtggagg
     8581 aaactttgct aaatcaatag ctgagatagc aggacttcag aatgcaactg gttctgacac
     8641 aagaggattt tgtgcagcac caactcatgc attaattcaa gcagcagctt tagtaaaagc
     8701 tggcatacat aaaaatgtaa tggtagtagc aggtggagca agtgctaaac ttggaatgaa
     8761 tgctaaagac catgttaaaa aaggtcttcc tgtacttgaa gatgttgttg gaggatttgc
     8821 agtacttgta tctgaaaatg atggtgtaaa tccagttata agaactgatt taacaggtaa
     8881 gcatactgtt ggaactggtt cttcaccaca agcagttatg acagcactta taacttcagg
     8941 tcttgataga gctaatttaa aaataacaga tgttgatgtg tattcagttg aaatgcaaaa
     9001 cccagatata actaagccag caggagcagg agatgttcca gaagcaaact acaaaatgat
     9061 aggggcactt gctgttaaac gtggagactt agaaaagaaa gaattgaaag attttgtttc
     9121 taacaaagga ttaccaggat gggcaccaac tcaaggacat ataccatcag gagctcctta
     9181 tataggattt ttaatagatg atttaactac aggaaataga aatagagcta tgatagttgg
     9241 aaaaggtagt ttattcttag gaagaatgac taacttattt gatggagtat cttttatagc
     9301 tgaaagaaat acaggtgtta ctgaagagac ttcaggaata tctaaggatg aaattaagaa
     9361 gataatagct gaatctatga aaaaactagc attagatatg ttagaagaat aggggtgagg
     9421 atatgtcaaa aaaagtaatc gcagatgtat tcttagaagt agctaatgca atagaaagcg
     9481 gcgaatttgg caaaaaagta aaaattggtg ttactacact tggaagtgaa catggtgtag
     9541 aaaacatggt taatggtgca caattagcaa aatctaattt atttgatatt gtgcttattg
     9601 gaccaaaagt ggaaactgac cttgaagtag ttgaagttaa tgatgaaaaa gaaatgcatg
     9661 caaaaatgga agaattatta gattcaggat atattgatgc ttgtgttact atgcattata
     9721 attttccaat aggtgtatct acagtaggta gagtaataac tccagcaaaa ggaaaagaaa
     9781 tgatacttgc aacaacaaca ggaacaagtg ctactaatag aatagaggca atggtaagaa
     9841 atgccattta tggtattgct acagctaaat caatgggtaa caaatgccca aaagttggaa
     9901 tacttaatgt tgatggagca agacaagttg agaagtgctt aaaagaatta aaagataatg
     9961 gatatgacat ggagtttgcg gattcaatta gagcagatgg tggatgtgta atgagaggta
    10021 atgatttact tgtaggtgca cctgatgtta tggttactga tacattatct ggaaatatat
    10081 ttatgaaagt attttcttca tatactacag gtggagatta tgaagctcaa ggctttggat
    10141 acggacctgg agttggagaa gactatgata gaaaggtact tatagtatca agagcatcag
    10201 gttctccggt agtagcaaat gctttaaaat acgcttatga tgttgtaaag ggagatataa
    10261 gtaatgttgc aagaaatgaa tttgcaaagg ttaaaaaagc aaaatttgat gacattatat
    10321 cctctcttac taaaaaagaa gttaaagctg aaaaagtaga agttaaaatg ccagataaag
    10381 aaattgttac tcgtcaaata gctggtgttg atataatgga tttagaagat gcagtatctg
    10441 agttatggaa aaacggaatc tatgctgaaa gtggtatggg ttgtacaggt ccaatagttt
    10501 tagtaaatga agctaaagga gacttagcag ttgaaacttt agttaaagct ggatatactg
    10561 caaaataagt acatttttta tatgtaaata agaacatata gttataattt tattgattat
    10621 aattatatgt tctttttatt ttatatcttg tcaatattta tatattaaca caatccattt
    10681 atttgattct ctagctaatt tattatattt attcaattaa aattaagctt tctaactaaa
    10741 taaaaactaa tgaagtatta aaatgaaatt aataaaatat gaaaaaattt taggaataaa
    10801 gtatctgtta gataaaaata agattaatta gtgatataat ataattaaga taaaatttat
    10861 tatttggagg taaaatcatg agcagagtaa aaaatgtagt aggattgttg ggaactaaag
    10921 gtgttgatgc actatatcta acaaagaaaa ctaatgtaaa ctatataagt ggttttcctg
    10981 atgaagaagc atatgcagtg atttgcaaag atggaaactt tttagttact gatagtagat
    11041 atatggagct tgcagaaaag gtttgcaaag attttgaaat tataaattgg cataattttg
    11101 atagaagtgt ggcaaaggca gttaaaagtg tatgtgataa agtgggtata aagaaacttg
    11161 ggtttgaaag aactaatata gtgtttgata aatatgaaga acttaaaaat ctgattgaga
    11221 aggataatgg agaattaata cctacagaaa atattgtaga gactttgaga tatgtaaaag
    11281 acaaagatga aatcaaaaat acaagaaaag cttgtgaaat agcggataag gcattagaag
    11341 aattgattcc acatataaaa gctggagtta gcgaaattga actggctaca aagttagagt
    11401 actttatgaa aatgaacgga gcacaaaata tagggtttga aactatactg atatcaggag
    11461 caaagacttc attgcttcat ggtaagccta gtgataagat tatagaaaaa ggtgattttg
    11521 tacttataga ctatggtgct atgtataatg gctatatatc tgatacaact agaactttta
    11581 tagttggagg agcttctgaa aagcaattag aaatatataa tttagttaag gaagctcaaa
    11641 atgtaggggt tgagaacatg aaagctggtg tacatgcgac aattccagat gctgagataa
    11701 gaaaagttgt caaaaaatat gaagactatt actatcaagg tataggacat ggagtcggta
    11761 gggatgttca tgaagaacct ttcattggta attatggtga taaaatcata gaagagggat
    11821 gtataataac aatggaacca ggtatttatt tccctggatg gggtggagtt agaatagaag
    11881 atactgtttt gattactaaa aatggtccag aaagacttac taaattccct aaagatttga
    11941 tgattttaga caaataattt aatttatata tattttttta cataaaatat aacataatct
    12001 cactattaag ctctatttac atattatagt aaagagggct taattttatt gtaagaggtg
    12061 atagtatata tgtataaaaa acataaattt ataaaaaata taaaaaaagc ctggtataaa
    12121 atcaaattaa ttgatagatg ccttataatt gtaatggcaa tattaatgtt tcaatctata
    12181 tataatttat ttgttaatga agtaaattca caagatacta caactattga tgtagtagta
    12241 agaaccactt cagcagctat atttgggtat tttttaagtg ctaattttat aaagagacct
    12301 ttaaggaagt atacaaatac tgatgtatct tcaaatcttt ttatagataa taatttaccc
    12361 ccaaaaggtc aagagtctag tcaaaataat ataatgaatg tcaaaaatac cattggattt
    12421 acttcagaag ctgataatta tgaaaagaaa atacctatta ataataatga aggttttgaa
    12481 gaaggagaaa ctagtgaatt gcaaattatt atagctactg taatttgtat agcagcatta
    12541 acagtacttt ttgttgtaag aaattttaca actacaacat cagcttcttt aggtactatt
    12601 tcacagatga gagattttgt ctcaggatgc gtgggttttt tgctaggttg tccaagtaaa
    12661 cctgattaaa aaagtattat caataaaagg tattttaaaa agtatcagta taataatatt
    12721 tagatttaaa tgtatacaat tataattcta taacattcta tattgtattt ttatactgat
    12781 actctaaact atactattta ttaaaatatt ttaacattgt atttaaacaa aatattatat
    12841 ctgtataata taaatttagt ttatatgaat agatgccata tactcaataa aaagtttcat
    12901 actatgagat atgtattgat ttcttctcca aagaactcca atatttgcag taattgcacg
    12961 ttctaatggt attgaacaga gattttctgt attttgaaat atttctttat acaatattgt
    13021 tgaagccaag tcattttcta gtaaataacg tatggtagaa agttgatttg agtgtaaaat
    13081 tatatttggc ttagcttttt caagtctttt tgtaataatc ttatagtgat aagaaccttc
    13141 agataagatt acaaaaggta cattttttaa catataattt gatataatgg gatgttttgc
    13201 caattcattt ttgggatttg taactaagta caattctgtt ttatagattg tctttgaatc
    13261 tatatcatca taaatatcac tgtcaattat accaatacaa aaatcaagtg tagattcttc
    13321 aatcatttta gctccaacaa atgttggaac ttcaaaaatt ttaagctcta tatctggata
    13381 aattacatta aatctaggaa gtatttttga aagcaaaaac gtacctaaaa ctgtaggaat
    13441 acctattttg agagaagctt ttctcttttt tcctaaatca agtgcgtcac attgaaaatc
    13501 ctcaaattgt tttataaaat gttttgactt gtctaaaaaa atttttcctt caggcgttaa
    13561 ttctatttta ttacctattc tgtaaaaaag ttcaaagcca agttcttttt ctaattcttt
    13621 aattgctaat gttatggatg gttgagaaat atgaatactt tccgcagcct tagtcatgtt
    13681 tttatattta cagacagttt gaaaatagta taaatagttt atattcattt ggtattagtt
    13741 cctttcattt acatattagt gacaagtata taattaacaa tagcataagt tttgtatatt
    13801 gttgtaaaaa aatagaatag ttttaaacta tcatagcata agtttttgat attttcaatc
    13861 tattaagaat agtgctatta ttaaattaac aaaagagaat agctggtatg gtttagagta
    13921 aatcaatcta tattacagtt atatcaacga atatcaaaaa attatctatt agaaaataaa
    13981 aattattttt acaattaaat tgttaattaa atcataaaga aaaaagtgat attaacaaga
    14041 taaaattttt ttaatcagaa atgttaattt aataacaaaa tagagtattg gggagaaagg
    14101 agaaatatat tttttaataa tatttgttaa tttaataacg aaaaatgaaa gggagtgatt
    14161 tagtgaagtt tgaattgatg gattttttaa tgaatccttt tgtccttatg tttgctgcag
    14221 taattacagg aattttattt ggaaaaatta aatttgggaa gtttaatttt ggggtatcag
    14281 gagcattatt tacaggatta tttattggat ggcttgcata tagcttgggg aatctaataa
    14341 tagcaaaagg agaaacagca gcaggatata aggcagcaac tgtaatgatg gggaatggaa
    14401 ttatttcttc tgacttcttt gatttctttt taataatatt tgttgcagca gttggtctat
    14461 tggcagcaaa agatatgaaa gctgtattaa agaagtatgg agcaagattt gtaattttag
    14521 gagttcttat aacttttata ggtggctcta tgacttatgc aatgacttta ctatcaagtg
    14581 ataaaggaag tagtgcttat gaagtttctg gcgtttacac aggagcatta acaagttcgc
    14641 ctggattagc tgctgcttta gaaacagctg gtaaacatgc agaagatgta tcaaaagaat
    14701 ttgaaaaagc ttcaataaaa gataaaaaag aaattttaaa agtagtcgac ccagaaggca
    14761 aattggatgt gaatacaact acatcactga ctcaagagca aatagataag tatattgcat
    14821 atgctgaagc tggtgttggt ataggtcatg cagtcgcata tccttttgga gttttaatag
    14881 taatcttagg tgtaaatttc cttccaaaat tatttaggat ggacctaaaa gaagaaagaa
    14941 gaaaatatga gaaggaaatg aaggaagcaa gagatagtgt ttctggtaaa acgataccag
    15001 aagttccatt taatataata acatttttct tgacatgttt agctggatac ttagttggag
    15061 gaatacatgt atttatggga ccactagggt acttcacact aggcgcaaca ggaggttcac
    15121 taattgtatc tctagttctt ggatatatag gtaagatagg ggtagtaaat ttcagaatgg
    15181 aagaaaaggt tttaaatatt ttaaaacaaa tagggctagt atttttctta gcaatagttg
    15241 gattaagata tggtggaaaa gtagttgatt caattatgac atctggtatg cacctagcct
    15301 tagtagcaat agcagtaggt gttacagcta tgatgatagg attcttagtt ggcaaatacg
    15361 tatttaaatt aaattggatt ttattatctg gagctgtctg tggtggtatg acatcaacac
    15421 caggtcttgg agctgctgtt gatgctcttg atagtgatga cccagcagca ggttatggtg
    15481 ctacgtatcc atttgcatta ttaactaaag ttatattggt tatagtttta cacaaacttc
    15541 caatgtaaat aagattttat atggggggat atttatgaat aatgaaggag catttgaaga
    15601 attaagtgga agattaagaa ataaagaatt gtgtgattta ataatgaatg cagatgaagc
    15661 tgctaaaatt atagaggatg gcatggttat tggagttagt ggatttacac cttcaggcta
    15721 tccaaaagca gtaccattag cagtttcaga aagagctaaa agtggagaaa agataaaatt
    15781 aaccgtatat tcaggagcat ctttaggccc agaagttgat ggagcatggt cagaagcagg
    15841 tataattgaa agaagacttc cttatcaaac taattcaata cttagaaata atattaataa
    15901 aggtgttgtt gattatatag atatgcacct aagtcattct actcaatttt taaattatgg
    15961 aactatacct aaagtagatg tagctatagt agaagcactt gcaataactg aagaagggaa
    16021 tattatacca actagtggga taggtaattc tccatctttt ataaaaagtg cagataaagt
    16081 aatagtagag ataaatcttg caaaaccaat ggaaatggaa ggtatggcag atatatatat
    16141 tacggaaaat ccaccaaata gaaaaccaat agaaataaat catcctaagg atagaattgg
    16201 aacaacatat ataccttgtg gtttggataa aattgcagga gtagtaatta ccaatatgca
    16261 agataagacc cgtccattgg gggttgtaga tgaagcttcg aaaaaaatat caaataatat
    16321 tattgctttt ttaagagaag aagtaaaggc aggcagatta tcaaagaatt tacttcctct
    16381 tcaatctggt gtaggaagtg tagcaaatgc agtattatat ggattatgtg aatctgaatt
    16441 tgaaaattta acttgttata cagaggttgt acaggactct atgttggatt taataagaat
    16501 gggtaaagtt acaatggcat ctactacatc tgtaagtcca tctccagaag gattaataaa
    16561 atttgaaaaa gatattgatt tttttaagga taagattata ttaagacctc aagaaattag
    16621 taacaatcca gaaatagcca gaagaattgg tgtaattgca atgaacactg caattgaagt
    16681 tgatatttat ggaaatgtaa attctactca cataatgggg tcaaagatga tgaatggaat
    16741 tggaggttct ggtgattttg ctagaaatgg agcaataact atatttagta ctgagtcgat
    16801 tgcaaaaaat ggtgatatat catcaatagt acctatggta tctcacgtag accacactga
    16861 acatgatgta atggtaattg taacagaaca aggatatgct gatttaagag gtcttgcacc
    16921 tagagaaaga gctataaaaa taatagaaaa ttgtgcccat ccagattata aagaacaact
    16981 tagggattat ttaaatagag cttgtaaaag tggagcaaaa caaacacctc acatattgga
    17041 cgaagcttta tcatggcata gtaaatttat gagtacaggt acaatgaaaa aggctgaaac
    17101 ttttaaaagt gcattataaa atatttaata aattggaggt agattttaaa tggaaaaagc
    17161 agtggaaaac tttgaagatt taagtaagga atacattaat ggttatattg agagagcaag
    17221 aaaagctcaa agagagtttg aatgttatac tcaagaacaa gtagataaaa tagtaaaaat
    17281 agttggaaaa gtagtgtatt ataatgctga atatttagct aaacttgctg tagaagagac
    17341 tggaatggga gtatatgaag ataaggtagc taaaaataaa agcaaagcaa aagttatata
    17401 taacaattta aaagataaaa aatcagttgg tataattgat atagatagag aaactggaat
    17461 aacaaaggta gctaaaccag ttggagtagt agcggcaata actccttgta caaatccaat
    17521 cgttacacct atgagcaatg caatgtttgc acttaaggga agaaatgcaa ttattatcac
    17581 acctcatcat aaagcaattg gatgtagtac aaaaactgta gagatgatta atgaagaatt
    17641 agaaaaaata ggagctccag aaaatttaat acaaatactt gaccaacaat caagagaaaa
    17701 tacaagaaat ttaatttcat cagcagatgt agtaattgcc actggaggaa tgggaatggt
    17761 aaaagctgcg tactcaagtg gaaaaccagc acttggtgta ggggcaggaa atgttcaatg
    17821 tatcatagat agagatgtag acattaaaga agcagtacct aaaatcatag caggaagaat
    17881 ttttgataat ggtatcattt gttcaggtga acagtcagta atagtagcag aagaaatgtt
    17941 tgataaaata atggatgaat ttaaaaataa caaaggtttt atagttagag ataaagtaca
    18001 aaaagaagct ttcagaaatg ctatgtttgt aaataaatct atgaataaag atgcagtagg
    18061 tcaatcagtt catacaattg caaaaattgc aggtgttgag ataccagaag atacaaaaat
    18121 aatagtaata gaagctgatg gaccaggaga agaggatatt attgcaaaag aaaaaatgtg
    18181 tccagttata tctgcataca agtataaaag ttttgaagaa ggtgtagcta ttgcaaaagc
    18241 aaatttaaat gtagaaggaa aaggacatag tgtatctata cattcaaata cagttaaaaa
    18301 tatagaatat gcaggagaaa atatagaagt atcaagattt gttattaatc aatgttgtgc
    18361 tactagtgct ggaggaagtt tctttaatgg tcttgcacct actaatactc taggttgtgg
    18421 ttcttgggga aataacagca tctctgaaaa tcttgattat aagcatctaa ttaatatttc
    18481 tagaattgct tattatatgc cagaaaatga agtccctaca gatgaagaac tttggggata
    18541 aaacttagac aaaaaatatt cattctaaat taaagtagga atatatttta atttagaatg
    18601 aatttaagac ataaattaaa tttaaattag tttattggac taaatttaaa atattaagag
    18661 acatatagaa gaaggaagta ttataattta aaataaataa aaaaatctat aaaagagaag
    18721 gagtggtttt attatggcat taatgacagg agctcaatat attgaaagtt tgagaaaatt
    18781 aaatactaag gtttatatgt ttggtgaaga ggttaagaat tgggttgacc atcctatgat
    18841 tagaccatct ataaattgtg tagcagcaac ttatgactta gctcatgacc cagaatatgc
    18901 agatttaatg acagttactt caaatatcac aggtgaaaaa ataaatagat ttggtcatct
    18961 tcatcaaagt gttgatgatt tgataaaaaa agtaaaaatg caaagactat gtggtcaaaa
    19021 aacagcttct tgtttccaaa gatgtgttgg tatggatgcg tttaatgcag tgtattcaac
    19081 aacatttgag tgtgataaag cccatggaac taattatcat gataattttg taaaatactt
    19141 aacttatata caagaaaacg atttagtagt tgatggagct atgactgacc ctaagggaga
    19201 tagaagtcta tcacctagtg ctcaacctga cccagatatg ttccttcata tagtagaaag
    19261 aagagaagat gggataattg taagaggagc aaaggcacat caaactggtt caataaattc
    19321 acatgagcac cttataatgc ctactataag tatgactgaa gcagataaag actacgctgt
    19381 atcatttgca gttccttcag atgctgaagg tgtgtttatg atttatggaa gacaatcttg
    19441 tgacaccaga aaattagaag aaggtgcaga tgttgattta ggaaataagg agtttggagg
    19501 tcaagaagct ttagtagtat ttgataatgt atttgttcca aatgatagaa ttttcttatg
    19561 tggagaatgg gatttctctg gtatgttagt agagagattt gcaggatacc atagacaaag
    19621 ttatggtgga tgtaaagtag gagtaggtga tgtaataata ggagcagcag ctttagcagc
    19681 tgattataat ggagcaaaca aagcttcaca tataaaagat aaactaatag aaatgactca
    19741 ccttaatgaa agcttatatt gctgtggtat agcatgttca tcagaaggtc acaaaacaga
    19801 agctggaaac tatcaaatag atttacttct tgcaaatgtg tgtaaacaaa atgtaactag
    19861 atttccttat gaaatagtaa gacttgcaga agatatagct ggagggctta tggttactat
    19921 gccttcagaa aaagacttta aatcagacct taaagttgga acaagtggaa tgactattgg
    19981 cgaagtatgt aacaagtatt ttaaagcttc ttcagtggct tcaacagaag aaagaatgag
    20041 aatattaaga ttcttagaaa atatatgtct tggttcatct gctgttggtt atagaactga
    20101 gtcaatgcat ggggcaggtt caccacaagc tcaaagaata atgatttcaa gacaaggaaa
    20161 cataaatcaa aagaaagaat tagctaagaa aattgcggga ataaaaaaag aggaagcttt
    20221 aaatttataa aaagttgata tataaaggga aattatatat taatgtgtat taattaatga
    20281 aaagatatgt agtataagag atagttataa aattatggtg gtttttagct aagtaaagaa
    20341 atttttaata aggagaattt acacatgacc tgtggagtta gattttgtgg gggctgtaac
    20401 cctagatacg aaagagggaa ggctcttaca ataatcaaag agcacttcaa tggtagggta
    20461 gattttagca tagcacaaga gaacgtcgag tatgatttgc tacttataat tggaggatgc
    20521 gcaactctat gcgcatccta tcaacaatat aaagtgaaaa atgaatgtgt taagatgtgg
    20581 gattttagcc atatagaaag agttataaat gtgattgaat cagcaatttg tgaaacttaa
    20641 aaaagctaaa tggggggatt tcattgagtt ggcaagaatt gtatcaaagt aaattatgta
    20701 gtgctacaga agctgtaaaa cagattaaaa acggtgatac tgtagtattt gcacattgtg
    20761 taggtgaacc tcctgcacta gtagaggcta tgattgaaaa tgctgaacaa tataaagatg
    20821 ttgagattaa acatatggta agcttgggta gtggtggata tactgctaag ggaatggaag
    20881 ctcattttag agtaaatcca atgtttgtaa gtggaaatgt aagaaaggca attgaaaatg
    20941 gagatggtga ttttacacct gcattcttcc atgaagtacc aaagttgctt agagaaaaaa
    21001 gattaaaatg tgatgttgtt ttagcacagg taactccacc agatgaacat gggtattgta
    21061 gcttaggaac atcagttgat tatacttatg aagccattaa aactgctaga acagtaattg
    21121 ttcaagtgaa tgaccagttt cctagaacct atggtgaggt agtccatgta tcagagtttg
    21181 actatatagt ggaaaaatca caacctttat ttgaactaca acctgcaaag attggagaag
    21241 tagaagaagc aattggtaaa aattgtgctt cattaattga agatggttct actttacagc
    21301 taggtattgg tgggatacca gatgcagtta tgttattttt aactgataaa aaggatttag
    21361 ggattcattc agaaatgatt tcagatggga ctttggccct ttatgaaaaa ggtgttataa
    21421 atggtaaata taaaaatttt gataaagaaa aaatgacagt taccttctta atgggtacta
    21481 aaaaattata tgactttgca aataataacc ctgcagtaga ggtaaaacca gtagactatg
    21541 tgaatcatcc agcaattata atgaaacaac ataagatggt ttctataaat tctgctatac
    21601 aagttgattt aatgggacag gtagttgcag aggctatggg acttagacaa tttagtggtg
    21661 ttggaggtca agttgacttt atacgtggag tgtctatggg ggaagatgga aaggcaatta
    21721 tagctatgcc ttcaataact acaaaaaaag atggtacagt aatttcaaaa atcgtatcta
    21781 ttgtggatga aggtgcacct ataacgacat caagaaatga tgttgactat attgtaacag
    21841 aatacggtat agcagaatta aaaggaaaat ctcttagaga aagagcaaga aatttaataa
    21901 atatagctca tccaagtgta agagaaagtc tagcagtaga atttgaaaag agatttaagg
    21961 agaaatatta ataatagagg ggtgttaata atggaagcac ttagagtagt tccaaagata
    22021 ttttattttg atacatttaa agaatttaat gaagaattta aaataggaaa aaatgattta
    22081 gtaattacta atgagtttat atatgagcca tatatgaaac ctcttggaat agatacaaat
    22141 ttaatttttc aagaaaagtt tggaacaggt gaaccatctg atgaaatgat agactccatg
    22201 acaaaggaaa tgaagaaata caattttgat agaattattg cgtttggagg aggaacaata
    22261 gttgatatat gtaagatatt agcacttgat gttccagaaa agtctattga tttatttgaa
    22321 ggagaagtat cacctaaaaa agtaaaagag ttagtagtag ttcctacaac atgtggtaca
    22381 ggtagtgaag taacaaatgt agctattgca gagttaaaat ctaaacatac taaaaagggg
    22441 cttgcagtag aagaaacata tgcagactat gcagtgttaa ttccagagac agtaaaaggg
    22501 ttaccttaca aattctttgt tacaagttcg gtagatgctc ttatacatgc tattgagtct
    22561 tatctttctc caaaagcaag tccatttaca gagatgtatt ctctacaagc aataaaaatg
    22621 attatggatg ggtataagaa gatagttgat aaaggagaag aagagagatt taatcatctt
    22681 agagactttg tacttgcatc aaattatgca ggaattgctt ttggaaatgc tggttgtgca
    22741 gcagttcatg cactatctta ttcaataggt ggagctttcc atgttgcaca tggtgaagca
    22801 aattatcagt tctttactga agtatttaag atgtattcta gaaaaaaacc taatggaaaa
    22861 attataaaat gtacaaaaat tttagcagat gcccttgaat gtgaccctaa ctgtgatgtt
    22921 tatggagaat tagaaaagtt tttaaataaa ttaatagcta aaaaagcttt gagagaatat
    22981 ggaatggtgg aaagtcagat agatgaattt acagatagta caatagcaaa tcaacaacga
    23041 ctacttgcta ataattatgt agagcttagt agagaggaaa taagagagat atttgcaaat
    23101 ctttattaat acaatttttt atttatatgt atagtcaaca taaacaatgc taattatatt
    23161 tatgtacaaa taaaaataag ggaacttaaa ttttaattaa agttaagttc tcttattttt
    23221 atatggagtt aaatataagt atatttttaa tttaaaatat gcttatattt aagtctatta
    23281 ctttatttga aacaatttgt agaatttcaa attccttgac agtaataagt aagtaattgt
    23341 ataatcaaac tgcaatatat taataatatt agtgaaaatg aaaggattat aaaatatgta
    23401 taaatcagaa aatttttcaa aaaaatctgt attaataaat tcatttttaa gtaaattttt
    23461 aaaaataatt ggatggggtt tggtaagctt atttacatta tcagccttag tactttcaac
    23521 tatttccatt ataattggat gggctgtaat acttcctatt caagtatgtg cagtattttt
    23581 acttatagga ggagtttgta tttttaaagg taagagatta ggagaccaaa tttctcgtta
    23641 taaaaaatat tgtacaatta taaacaatag aaatattatt ccagtagaac ttattgcaga
    23701 aaacacatct aaatcattaa attttataat aaaagatgta caaaagatga tagacaaaaa
    23761 ctactttata aatacttata ttgataaaag aaataatcag attgtgttaa caaatgaaga
    23821 ttttattcca ccaagatatg aagatgtagc atatgaagtc aaagaagaag aaaagactga
    23881 tgaatccaat gaagttgata caattataaa taaaggaatg aattatctaa cacaaataaa
    23941 agaagctaat aaaaatatta aatcagaatc aatgggcgat aaaattgtac aagtagaaga
    24001 tgttacaagt aaaatatttg atgttgtaaa acatgaccca tcaaaactta ctcaaataca
    24061 aaaatttatg gattattatc ttccaaccac tttaaaacta ttaaattcat atcatacatt
    24121 ggaagaacaa ggaattgata gagaaaatat aacaacaact atggaaagta ttgaaaatac
    24181 aatgaatacg attgtagtag cttttgaaaa tcaattggac tatctgtttg aagatgaagc
    24241 cattgatatt tctacagata ttactgtttt agagaatatg ttagtccaag aaggattaac
    24301 gagtggtaaa ttttaacttc aatagaaaaa atattctgtt atttttaaac aattaattag
    24361 tcataaagta taaaatgtgt attatataag ttaatcagta ctttatgaag gaagaggaga
    24421 gaaagataca atgaataatt tagatgatat accagttatg ccaactttga cacttgaccc
    24481 atttggagaa gaatcaaaca ctaatgatat agataattct gacttattaa tgaaaaagga
    24541 tgaaaaagac ccagaagaag aaaaactgtc tgaatctgaa cgcaagatgg taaaggaatt
    24601 tgcggaaaaa atagatatta caaatacaaa tatgattttg caatatggag ctggtgctca
    24661 aaaaaaggta gcaagttttt ctgaaacagc tttaaagagt gtaaaaacaa gagatttagg
    24721 agaagttgga gatatgctta caaatctagt tactgattta aaagcatttt cagcagatga
    24781 aaaagaacaa agtggttttt taggaatttt taaaaaggca aataataaaa tttcaaattt
    24841 aaaaactaag tatgatagtg cagaggtaaa tgtggataaa gtaagtaaag agcttcaaaa
    24901 gcatcaggtt aaaatattga aagatatagc aatgcttgat aagatgtatg aattgaactt
    24961 agcttactca aaagagctta ctatgtatat tatagcagga aaacaaaaat taaaggatat
    25021 gaaagaatat gaaatgccaa agttgagaga aaaagctcgt ttatctggtt caacagaaga
    25081 tgctcaatct gtaaatgata tggtttcact ttgtgacaga tttgaaaaga aaattcatga
    25141 cttagaatta actagaatgg tatcgcttca aatggctcct cagataagat tggttcaaaa
    25201 tagtaataat ctgatggcag aaaagattca atcaactata gtaaatacca ttccattatg
    25261 gaaaaatcaa atagtgcttg ctcttggaat ttctcactta aatcaagcta tgaaagcaca
    25321 gcgtgaagtt agtaatatga caaatgagct tttattgaag aatgcagaaa ctttgaagat
    25381 gggaacaata gaaacagcaa aagaatcaga aagaggaatt gtagatatag acacaattaa
    25441 gaaaactaat caatctctaa tatctactat tgatgaggta gttaaaatac aacatgatgg
    25501 tagaataaag agacaagaag cagaagttga attatcaaaa atagaaaatg aacttaaaag
    25561 taagcttctt gaatttacag ttaaataaaa attatactaa tataaaaaga gactgtctca
    25621 aaatagagat aagtctcttt ttcattgttt aaatgattaa agtatattta tattataaat
    25681 ttctattatc ttttaacata acatcatgag ctccaccttc aactatacta gtagaagata
    25741 caagtgtaag tttagcattt ttttgaagtt caggaatgct aagtgctcca cagttacaca
    25801 tagtagaacg aactttactt aaagaaagag ttacattgtc tttcaaagaa ccagcataag
    25861 gcacataaga gtcaacacct tcttcaaaag aaagtttctt atctccacca aggtcatatc
    25921 tttgccagtt tctagcacga gcagaacctt ctccccaata ctctttcata taactaccat
    25981 ttatattaac tttgttagtt ggactctcat caaaacgaga gaagtatcta ccaagcatta
    26041 taaaatctgc acccatagct aaagctaaag ttatatgatg gtcataaacg ataccaccat
    26101 cagagcaaat tggaatataa atacctgttt tttcaaagta ttcgtctcta gcttgagcaa
    26161 cttcaattat agaagtagct tgtccacgtc ctataccttt ttgttcacga gttatacata
    26221 tagaaccacc accaatacca actttaacga agtcagcacc agcttcagct aggtaaagga
    26281 atccctcgcg gtcaactaca tttccagctc ctaccttaac tgtatcacca tatttttcac
    26341 gaatgaaatc aagtgttatt ttttgccact cagaaaaacc ttctgaagaa tctatacata
    26401 aaacatcagc accagcttca actaaagcag gaacacgttc agcaaagtct ctagtattaa
    26461 taccagctcc aactacatat ctcttagaag aatctaaaag ttcaagtgga ttttctttat
    26521 gagaagagta atctttacgg aataccatat acataagatt accattatca tcaagtacag
    26581 gaagagaatt aagtttgtgg tcccatataa tgttgtttgc ttcctttaaa gtaacatctt
    26641 tgttagcata aactatagat gacataggag tcataaattc actaacttta gtatctaaat
    26701 ccatacgact tatacgataa tcacgacttg caacaatacc caataattta ccagttgaag
    26761 ttccatcttc agtaacagct actgtagaat gtccagtttt ttcttttaag tcaagtatgt
    26821 cttttaatgt gttatcaggt ttaatatttg aatcacttac aacaaaacca gctttgtggg
    26881 atttaacacg tgctaccata gcagcttcat tttcaatagt ttgagaaccg tatataaatg
    26941 aaataccacc ttctttagca agtgcaatag ccattttgtc atcagaaaca gattgcataa
    27001 tagcagaagt caaaggtata ttcatataaa tatctgcttc ctcgcccttt ttaaatttag
    27061 taacaggtgt ttttaaactt acattagcag gtatacaatc agtagaagag aaacctggta
    27121 caagaaggta ttcactgaaa gtatgtgatg gtgtatcaaa ataaaacgcc attttttaat
    27181 cactccttat atttttatac tttttataat tgatagtatt aattataact tattctttaa
    27241 ttttattaaa gtattttgtc aaatattgag atttttgaaa aatttaaact attttaatta
    27301 aatttgttta atatatattg ccttataggg taaagttgta tattaaataa tgtttagtgt
    27361 ttaatagtag ctaatttaaa attaatcttt agtgattcat ataattacat ttgtatagtt
    27421 tatattgata atattactag agagtgcata aacctcaaaa atacagataa attaaatcta
    27481 tattagtata tccaaaagta catttaataa att
//
LOCUS       DAMJVX010000033        27274 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMJVX010000033 DAMJVX010000000
VERSION     DAMJVX010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27274
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.035"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            246..779
                     /gene="pyrR"
                     /locus_tag="PC407_001350"
     CDS             246..779
                     /gene="pyrR"
                     /locus_tag="PC407_001350"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDF2496805.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            1063..2274
                     /locus_tag="PC407_001351"
     CDS             1063..2274
                     /locus_tag="PC407_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDF2496806.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLLLRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            2573..4225
                     /locus_tag="PC407_001352"
     CDS             2573..4225
                     /locus_tag="PC407_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2496807.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEYLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGLLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLLEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGCFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYIISRNKKTPSRDKQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQITKLKNSF"
     gene            complement(4440..6215)
                     /locus_tag="PC407_001353"
     CDS             complement(4440..6215)
                     /locus_tag="PC407_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDF2496808.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEIANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIVLSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            6511..7545
                     /gene="ltaE"
                     /locus_tag="PC407_001354"
     CDS             6511..7545
                     /gene="ltaE"
                     /locus_tag="PC407_001354"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDF2496809.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            7832..8662
                     /locus_tag="PC407_001355"
     CDS             7832..8662
                     /locus_tag="PC407_001355"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDF2496810.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKINLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGVLEV
                     "
     gene            8666..9547
                     /locus_tag="PC407_001356"
     CDS             8666..9547
                     /locus_tag="PC407_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDF2496811.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            9577..10194
                     /gene="gmk"
                     /locus_tag="PC407_001357"
     CDS             9577..10194
                     /gene="gmk"
                     /locus_tag="PC407_001357"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDF2496812.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            10197..10463
                     /locus_tag="PC407_001358"
     CDS             10197..10463
                     /locus_tag="PC407_001358"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDF2496813.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            10468..11667
                     /gene="coaBC"
                     /locus_tag="PC407_001359"
     CDS             10468..11667
                     /gene="coaBC"
                     /locus_tag="PC407_001359"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDF2496814.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLAFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            11906..14431
                     /gene="priA"
                     /locus_tag="PC407_001360"
     CDS             11906..14431
                     /gene="priA"
                     /locus_tag="PC407_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDF2496815.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNEIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            14449..14889
                     /gene="def"
                     /locus_tag="PC407_001361"
     CDS             14449..14889
                     /gene="def"
                     /locus_tag="PC407_001361"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2496816.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            14904..15833
                     /locus_tag="PC407_001362"
     CDS             14904..15833
                     /locus_tag="PC407_001362"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDF2496817.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTIYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            15843..16595
                     /locus_tag="PC407_001363"
     CDS             15843..16595
                     /locus_tag="PC407_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HDF2496818.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNVGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIDFLTGKNILICN"
     gene            16664..17365
                     /locus_tag="PC407_001364"
     CDS             16664..17365
                     /locus_tag="PC407_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDF2496819.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            17427..18752
                     /gene="rsmB"
                     /locus_tag="PC407_001365"
     CDS             17427..18752
                     /gene="rsmB"
                     /locus_tag="PC407_001365"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDF2496820.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            18760..19791
                     /gene="rlmN"
                     /locus_tag="PC407_001366"
     CDS             18760..19791
                     /gene="rlmN"
                     /locus_tag="PC407_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDF2496821.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGNIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            19811..20563
                     /locus_tag="PC407_001367"
     CDS             19811..20563
                     /locus_tag="PC407_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDF2496822.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            20580..22595
                     /gene="pknB"
                     /locus_tag="PC407_001368"
     CDS             20580..22595
                     /gene="pknB"
                     /locus_tag="PC407_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDF2496823.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     EEEEEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGSSNSGNSGGNSGNNGGNSGGSNSGNSGGSSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            22703..23440
                     /locus_tag="PC407_001369"
     CDS             22703..23440
                     /locus_tag="PC407_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2496824.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVENMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            23538..24422
                     /gene="rsgA"
                     /locus_tag="PC407_001370"
     CDS             23538..24422
                     /gene="rsgA"
                     /locus_tag="PC407_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2496825.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            24427..25098
                     /locus_tag="PC407_001371"
     CDS             24427..25098
                     /locus_tag="PC407_001371"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2496826.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            25107..25748
                     /locus_tag="PC407_001372"
     CDS             25107..25748
                     /locus_tag="PC407_001372"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDF2496827.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTVSVTAI
                     KGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            25879..26508
                     /locus_tag="PC407_001373"
     CDS             25879..26508
                     /locus_tag="PC407_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2496828.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            26563..27219
                     /locus_tag="PC407_001374"
     CDS             26563..27219
                     /locus_tag="PC407_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2496829.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGASIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT        10859 a         2765 c         5090 g         8560 t
ORIGIN      
        1 atttgaaata taaatatata atttgagttg atagattaaa aaattatctt taaacgatac
       61 agagatatcg taagacaatt gctaaattac actgtatatt ttttatataa gagcttcttg
      121 tcttgtgatg agaagttttt tgttgtttaa attgaaattt taaataaaaa taaatttata
      181 aaaaattaaa agaaaggagt agatttgtca aatagatata ttatatcttt gacaataatt
      241 atgatatggt agaaaaagct caattaatgg atgaaaaggc aatagcaaga gcaattacta
      301 gaataagtca tgaaataatt gaaagaaata aaggtgtaga aaatctagtt ttagttggaa
      361 taaaaactag aggtgtgcca attgcaaata gaatttctaa gaaaatagaa caaatagaag
      421 gaacaaaagt agatacagga gatattgata taactttata tagagatgat ttggaaaaaa
      481 ttcatgttga acctgtagtt aaaggaactt atttagattt taatgtaaat gataagacag
      541 tagttttggt tgatgatgtt ttatacactg gaagaactgt gagagcttca ctagatgcga
      601 ttatagatat aggaagacct aaatcaatac aattagcagt tctagtggac agagggcata
      661 gagagttgcc tataagagca gattatgtag gaaaaaatgt acctacttct agacatgaaa
      721 ttatatctgt cagtttatta gaaattgatg gagaagattc agttacaatc aaagaataat
      781 tagttttaag tattatataa aagcttagca ttgaatagtc agggaggagg ataaagctta
      841 catagattgc acaaaataaa tattataatt ttatggatgt gagtcttaat gtagttttta
      901 ttctccttta tttgagtttt tgatgtcgta aaatgtaaaa taatgataaa ttatataata
      961 agtatataga ggttaaaaaa tagaatctag ggaatgataa ttaattaaaa gatattaatt
     1021 aaacagagga tttatttata tataataatt ttggaggttt ttatgaaaaa aacaataatg
     1081 tctttacaac atctattagc catgtttgga gcgacagtat tagttcccat tttgacaggt
     1141 tttaatccgt cagttgcaat tttctgtgct ggtgttggta cattgatatt tcatttttgt
     1201 acggaaggaa aagtacctgc ctttttaggt tcaagttttg cgtttatacc agttatacta
     1261 gcagctaaag aggcgtatgg tggagattta gcatatgctc aaggtggtat aatagtagca
     1321 ggattaattt atattataat gtcgattata gtaaaaatag ttggtgtaaa taaaattaaa
     1381 ttatattttc ctgcacaagt tacaggagct atgatagtag ttataggatt aaatttattg
     1441 ccaacagctt ttagtatggc ttcagctaat tttgtaatag catttataac tttagcaata
     1501 gcaattttaa ccaataagtt tggtagagga tttataaaac aattaggaat attaatagcc
     1561 gttttttctg ggtatataat ctgtttactg ttaaggttag tagatataac tactattaca
     1621 gaagcaagtt tatttgcaat accgaacttt acagttccta agttcagttt aggagcaatt
     1681 gtgattatat ctccagttgt attagctgta tttatggaac atataggaga tatgactact
     1741 aatggtgctg tagtaggtaa aaactttata gaaaatccag gattaaatag aacattactt
     1801 ggggatggat ttgcgactat agttgcaggg tgtttaggag gaccagccaa tacaacttat
     1861 ggagaaaata cagcagtact tgctataact aaaaactatg acccttcaat acttagacgt
     1921 acagcaatat ttgctatatt gcttgcatgt gtaggaaagt ttggtggatt tcttcaaagt
     1981 ataccaggtt cagttatggg tggaattagt ataatgctat tttcaatgat aacttatgta
     2041 ggattaaaaa ctataaggga cagttcttgt gtagaaagta agataaatat attaataata
     2101 gcagttatat tattaatagg tcttggaaca acatatttat ctaataaagg catttctatt
     2161 ggaatcccta taacaagtac agtaaagata actggtctta gtttagctgc aattgttgga
     2221 attgtattaa acagaatttt aaataatcag gattttaaag ttgaagaaga ataaatttaa
     2281 agaataaatt gtatattgaa taactaaaat tagttaattt tctatagaca tttgtaatta
     2341 atgttgatat aatgattaaa aataaatgaa agaaagggca aggtaactag gacaatgaga
     2401 atataatcta ttttttataa tttaaagtat tgtaattttt gaaaagccta ttttgatggc
     2461 tttatttgca atgcttaaat taaggagagt agattatagt ctatacgttt atttgttatg
     2521 cctatttata gaatctctcc ttatatttaa gtgtgttaag gagggatttt ttatgttgtt
     2581 agtaaaagta gagaatttaa agaaatatta tgcagataaa ttaattttgg atatagataa
     2641 acttgaaata ttagaaaatg acaaaatagg tctagttggt tcaaatggtc aaggaaaaac
     2701 aacattatta aaagcaatat tgggtgaaat agaaattgat gaaggatata cttaccttac
     2761 agaaagctat tcttatataa gtcagagtga aaataatatt gaaacatgta gtcatagtaa
     2821 ggagaagagt cttttaaatg ctccagataa atttgaagaa tacttatcag gaggggaaaa
     2881 ggttaagtta aaaatagcag atgcgctgag taataagaaa aatatcataa tagctgatga
     2941 gccaacttca aacttagata aaaaaagcat tgggcttttg gaagatatgt ttaaaaggca
     3001 tgagggagca ttattattaa tatctcatga tagacgtttt ctggatgagt tatgtacaac
     3061 tatattagaa ctagaagatg gaaaactaaa agcttacaaa ggcaattaca ctgattattt
     3121 aatgcaaaaa gatgaagaag taaaaagagc tgattttgag tatcaagaat atgttaaaga
     3181 gaaaaaaagg cttgaaaaag ctcttttata taaaaaagct ttaagtgatg gcataagaaa
     3241 gacaccaaaa agaatgggta attcagaagc taggttgcac aaaatgggtg gtcagactaa
     3301 taagaaaaag ttggactcaa atgtgaaggc tataaaaagc agaattgata aacttgaggt
     3361 aaagaataaa cctaaagttt ctaaagaaat gaacattaag attcaagatg gtatggagat
     3421 aattagcaaa aatctattag aggtaaaaga tatgactcta aagttagaaa ataagctctt
     3481 gttagataat gtttccttta agataaaaag aggtaaaaag atagcattat taggtgataa
     3541 tgggtgtgga aaaagtactc tgataaaaga aatattggct gataaaaatg acaatataaa
     3601 aataaataac aaggtaaaag taggttgttt tgaccaaaat caaagtttat tagatgagga
     3661 aaagagtgtt ctatacaata ctaaagttaa tagttcattt gatgaatctt ttataaggat
     3721 aaacttaagc ctatttgggt ttaaaggtga tgatgtctac aaaaaagtaa aagtacttag
     3781 tggtggagaa aaggtaaaaa tagcactatg taaaataata ttggaagata ataatttttt
     3841 agtatttgat gaaccaacaa attacttaga tataaaatct atggaagcac tggaaaaggc
     3901 actgataaat actgataaaa ctatgcttat agtgtctcat gatagagtat ttgtatcaca
     3961 tatttgtaac tatattatag agataaaaga tgctaaaata agggagtttg attgtaatta
     4021 tgacgaatat ataattagca gaaataaaaa aactcctagt agagacaagc aaatcaaaaa
     4081 agagaatctt ctagtattag aaaatagact tacaactgtt atatctatgt tatctataga
     4141 aaaagataat ctaaaaaaag aattgtatga atcagaatat aatgaattac taaaacaaat
     4201 cactaaatta aaaaatagct tttagtgtta actttttaat tacatctata taagaagact
     4261 gaaaatattt gttgggaata taagtatagt tattaaaaca gaaaagtatt ttgttagaaa
     4321 attaaaagta agctaattag aaagtggcct tattattgta ctttataaga aggccactat
     4381 ttttatttag aatgataatg tgtgcttgtc taacatttat tttgtattta gtgcagaatt
     4441 tattcagatt taaccttaag cttggctact gtttcttctt caggagtaac ataaatagta
     4501 ctgttagttt cataaataac catacctggt ttagaaccac taggtttttt cacatttttt
     4561 ctcttagtat agtctacagg cacctgagaa gaaagttttg acttactaaa gaatgctgct
     4621 agcatagctc cttcaaatac agtattatca gggacttcct taccagcaca ctttattatt
     4681 acatgagaac caggaatatt ctttgtatgc atccataagt catcattatc agcaagtctt
     4741 agtgtaagat agtcattttg cttattgttt ttaccaacca atattttaaa tccatcagaa
     4801 gacacaaatt catgaggttt tgtagatggg atagtatcct ttttactgtt tttcttttga
     4861 gtcttgatat atccaacctt agcaagttct tcttttatat cttgtaattc agctaaattt
     4921 tcgcagtttt caatacttaa aactatgttt tctaagtaat ctatttcttc cttatttaag
     4981 gaaatttgat gagatatctc gactttagca tgtttcattt tgttatactt tttaaagtat
     5041 ttttgagcat tttcagatgg tgtcaagttt ttatttaatt ctattgttac atcattacaa
     5101 ttttcatcat agaagttagc aatttcaata ctttccatac ctttttctac catataaata
     5161 tatgaagtaa tcaattctcc ttttatctta taaatgtctg cattttcgga ttctgataat
     5221 tcttcttctt gttttttaag tttattataa agtctatcaa gcttaacaga tatactcttc
     5281 tttaaatcag atgaacgttg attaattctg tctttaatat ctttagttct atagaaatct
     5341 tctagaatac gagacatact atccttattt atatatgata aatcgctaaa taatgtaagg
     5401 tttatacaac tgaaatcaac taccttatcc acctttttat ctataataat gcaaggggag
     5461 tatttgttag aatttatatc attgaataaa ttacaaaata ctttgtgtaa agaatcaaat
     5521 tgttcatcag atatatcctt tatgatagca ttttgattta cacctgctct gtagcaaatt
     5581 tctttagcta taatagggct tatacctaga aatttagaat atatagactt aaagataggt
     5641 ccttccaatt cttctaggtt tttaataaac aaatccttag atatatcatc aagagggttg
     5701 agtttgtttt gttctggtgg taatgaataa tcatgaccag gtaaaacttg acgaactcta
     5761 cttatgctaa aagggactct ttttatagaa tctataattt tattttcttc accatgagtt
     5821 attattatgt tgctatgtct tcccataatt tcaatcataa tatttttaac tgtcttttct
     5881 tttaattcat ctaaagattc aacacttatt tttattattc tttcaaaacc tatttgagaa
     5941 atttctacta tatttccacc ttgtatgtat tttctaagta acatacaaaa cataggcgct
     6001 tttagagggt tttctttttg ataattactt gtaagataaa ctctaggatt tgaggcactt
     6061 gcacttaaaa ccaatctaaa attttcttta ttatttctta tattaaaaat tacctcatca
     6121 tcttcaggtt gatgtatctt atctattttt ccacctgtaa gttttgatga taattcatcg
     6181 actattgaat gtataactaa tccatctaat gccatttata gcaccccttt ctttgaccaa
     6241 aatgagtcag taaattaatt atacacataa tatacgcttt gtgactaggg ggaatttaaa
     6301 cgatagagat taatatttaa attgtataat tatttatact tattagagag gaaactttat
     6361 ttttaaaatt aataatttaa agtagattaa atataaagta aatatagtat aattattgtg
     6421 tagagtataa gctatgaaag tatatgaact ttacagaaaa aatcaatttt aaggttctat
     6481 agtatataaa tatgaaagaa ggttgaaaaa atgagattta tcgatttaag aagtgatact
     6541 gttactatgc ctactgacga aatgagagaa gctatggcaa aggctcctgt tggagattca
     6601 atctttagag atgaccctac tgtaaataaa cttgaagaat tagcagcagc taaagttgga
     6661 aaagaagatg ctattttcct tccaagtgga acttttggga atcaattagc tctatttact
     6721 cattgcctaa gagggcaaga aataattatt ggtaaaggtt atcacattgt aactcatgag
     6781 gttggggcac ctgctgtaat agctggagtt cagcttagaa ctatagatga agacgaaagt
     6841 ggagctctaa atcctgaact tgttgaaaag gcaattagaa gagatgacat acatgaacca
     6901 cagacagggc ttatatgtgt agaaaatgca tattcaggtg gaactgttgt gagtcttgat
     6961 aatttaagag aaattaaaaa aatagctgag aaacacaact taccagtaca tttagatgga
     7021 gcaagattat ttaacgcagc attaactttg ggtgtagaag ctaaagaaat agcaaagtgt
     7081 tgtgatagtg ttatgttctg tgtgtcaaaa ggtttagctg caccaatggg ttctatactt
     7141 gctggaagta aagaatttat agcaaaagct agaagaaagc aaaaagttat gggtggggga
     7201 atgagacaag taggtatagt tgcagcagct ggtataattg ccattgaaaa aatgacttta
     7261 agattgaatg aagaccatga aaatgcaaaa tatttagctt gtgaattaaa taaaattgat
     7321 ggcattgaag taaacaatgt taatccagat ataagtatgg tattttttaa gatgagtgaa
     7381 gatattataa aagaggatgt tttaataaaa gaattttttg aaagaaatat aaaaattaat
     7441 aaaatggaaa atggtgaata tagatttgta actcatgtag atattactaa ggaagattta
     7501 gactatgttt taaaaacact taaagaatta ataggtgtat gttaatagat atctactaaa
     7561 aggattatag ctggttacca gatgtttaat taaaaaattc atagacagtt taaataaaac
     7621 atacataaaa ttcatataga aatattaata tataaacata gaaagaacta gcttaaaaac
     7681 ataatataat taccctaatt gtaatgacaa ctctccctca catcctcaaa ttatagttgt
     7741 cattacaaaa cttttcccat aaattataag tttatgggaa tttttttatg ttataatagt
     7801 ttaagcgtta atttacgaag gagcgatact tatgaaattt tggaaactac atggaatagg
     7861 aaatgatttt atagcaatag atggaagatt cgaccagata aatccatctg attatactgc
     7921 tttagcaaaa aaagtatgcc atagaagatt ttctgtagga gcggatggat tgttagttgt
     7981 gaaagattca aaagttgcag atgtagaaat ggtatattat aattctgatg gttcaagagc
     8041 agctatgtgt ggaaatggcc ttagatgttt ttgtaaattt gtttatgata attgtatagt
     8101 taaaaaagaa agttttacag tagatacatt agatggtata aaagatataa aaataaatct
     8161 taccaataga gaaataaact ctataagagt caatatggga aaaggaagtt ttatagctaa
     8221 ggatgttcct gtacttacaa ataaagaacg ttttgtgcaa gaaagtataa aggttttaga
     8281 tagggagctt aaagttactt ctatgttgat gggggttcct catacagtag ttatagtaga
     8341 tgaattagat gtagatgatg tttgtaaata tggaaaagag attgaaaata ataaaatatt
     8401 tcctgaaaaa acgaatgtaa attttgtaaa ggtagaagat aagtataata tacatgttta
     8461 tacttgggaa agaggttgtg gatatacttt aggttgtggt actggtatga ctgcatcagc
     8521 aatagtgtgc aatttactta atatggtaga aagtagtgta aatgtaacgt ctcaaggtgg
     8581 aacagttaaa atagatgtag gagatttctc ttatatgaca ggtccagcag taaaaatatg
     8641 tgaaggtgtt ttggaggttt aattaatggc tataagtatg actggttttg gaagaggaga
     8701 atacaaggat gataattatt actttttagt agagtgtaaa acgataaatc ataagtattc
     8761 agatataaac atacgtcttc caagaaaaat ttcattttta gaggataaag ttagaaattt
     8821 agtaaaaaat tatgtaaaaa gaggaagagt agatttatac ataaagtttg acttattagg
     8881 caaagaagat gtaaacttaa attttgatga gggattagca tctcagtata tagacatact
     8941 aaaagaaata aaaaataaat ttgatattat agatgatatt agcgttatga atgttgcaaa
     9001 gtttcctgat attgtgaaaa tagaagaaaa ggaagaagat gaagatttat tgtggtcaat
     9061 gttgaatcaa gcagttgaag atgcattaat caaattaaga gaaatgagaa gtgaagaagg
     9121 taaaaaatta gctgaagata ttgccatgag atgtgattta ttaaaaaatc atatagaaga
     9181 gattgaaaaa tattcaagta gtgttgtaga agattataga gaaaaattga atttgagaat
     9241 aagtgaatta ctggatgacc caagtataat agatgagaat agattagcac aagaagttgc
     9301 aatatatgca gataaaagca gtataacaga agaaatagtt agatttaaaa gtcatatagg
     9361 gcaacttaaa aatactatat ttaaagacga ctctataggt agaaaaatag atttcttaat
     9421 acaagagatg aatagagaaa ctaacactat aggttcgaag tcatctgata taaatataac
     9481 taatttagta gtagaagtaa aaagcgaatt ggaaaaaata agagaacaaa tacaaaatat
     9541 agagtaagaa tacaatatta gaaggggaat gcttaaatgt ttgtgaaaaa aggattattg
     9601 ctagttgtat caggaccatc aggtgcagga aaaggtacta tttgcaaaga actacttaaa
     9661 gaaaatgata caataaaact ttctgtatca gctactacaa gaaaacccag aactggagaa
     9721 gttgatggag taaattattt cttcataagt aaggaaaagt ttgaagagat gatagaaaaa
     9781 ggtgagtttt tggagtatgc tcaaatttat gataattttt atggaactcc aaaagcggca
     9841 ataatggaat gtttagaaaa aggacaagat gtattactgg agattgaaat gcaaggagca
     9901 aaacaaataa aagaagtttg cccagaggga gtatttatat ttgtactacc accatcactt
     9961 gaagaattaa aaaatagaat tgttggaaga ggtacagaaa cagaagctga aatagagaag
    10021 agatttagct gtgcatatga agaaattaaa atgattaagg attatgatta ttttatattt
    10081 aatgaagatg taaaaacatc tgctaaagaa atagaaggta taatatcatc tgaaaagaat
    10141 aaagttagca gatataaaaa tattataata gagaaattta aggaggaatt ataattatgt
    10201 taaaaccatc tatcaatgag gtactagaaa aaatagacaa cagatactat ttagtaggga
    10261 ctgtttcaaa gagagcaaga aaattaattg atggagaaga accatatgtt tcaaataaaa
    10321 ctaaggaaaa gcctgtatgt gtagcaacta aagaagttgc aagtggaaaa ataacatata
    10381 gattattgac agaagaggaa atagagatag aagaagctag acatcatgct gagcaacacc
    10441 aacaaatttc agaagaggag taaggtcatg ctaaaagata aaactgttgt aattggcata
    10501 agtggcggta tagctgcata taaggcttgt gatgttgtaa gtaagttaaa aaaactaaat
    10561 gcaaatatcc atgttataat gacaaaatca gcaacagaat ttgttaaacc acttgcattt
    10621 caatcgctga gtcaaaatta tgtagtagaa gatatgtttg aagaaccaaa aacttgggaa
    10681 gttgaacata tatcattagc aaaaaaagca gatttattct taatagttcc agcaagtgca
    10741 aatgttatag gcaagattgc aaatggtata gctgacgata tgttgacaac tactgttatg
    10801 gcaaccaaag cacaggttct tatagcacca gctatgaata ctaatatgta tgaaaatcca
    10861 attgttcaaa aaaatataca aactttaaag agctttggat ataaatttat tgaaccagaa
    10921 agtgggaggc ttgcatgtgg agatattgga acaggcaaat tagctaatcc agacataata
    10981 gtagaggaag ttttgactaa tcttactaaa gagcaagatt taaaaggtca aagtatgata
    11041 gttacagctg gtccaactgt agagagtata gacccagtaa gatatttaac taataggtct
    11101 tctggtaaaa tgggatattc tatagcaaga gaagctatag ctagaggtgc tgatgtaaca
    11161 ttaatatcag gtcctacaaa tctttctatt ccacaaaatc ttaaaaaatt tgttcaaata
    11221 gagtcagcac aagatttgta tgaagcagta gtatcaaatt taagtgaaaa taaaatagta
    11281 attcaaagtg cagcagttgc agattataaa ccaaaaactt attctgacaa aaaaattaaa
    11341 aagaagaatg atgatttatc aatagaacta agtagaaatt atgatattgc ttatgaaata
    11401 ggaaaaataa aagaagataa gatactaatt ggatttgcag ctgaaactaa tgaccttata
    11461 gaacatgcca aaggaaaaat aaaaaagaaa aatttagatt ttattgtggc aaatgattta
    11521 actaaagaag gtgctggttt tagagctgat actaatattg taaaaataat agatgtagag
    11581 ggaaatatta ctgagtatcc taagatgaaa aaagaagaag tagccaatgt tattttagat
    11641 aaagttaaag cgttgcttga gaaataagac cttttttaaa tatgatattt gtgtatttta
    11701 tcacagctaa agtggctgaa agctgcaatt taattttagc gagtaaaaac tcgcaaatgt
    11761 cagttaaatt gtagttttca gcatttattt tgtcataaag tataaaatat aattaatagt
    11821 taattattaa aataaagtta atattaataa cttgaaattg cttataaatt ctgagatttt
    11881 aatatcttgt aaagtatatt aaaatatgaa caatatatta aatttaagta gaggttttat
    11941 aatgaaaaaa tatgcaaagg taatagttag aagtaatact atatatacag ataatttatt
    12001 tacatatcaa attcctgtat ttttatctga tgtaataaaa atcgggcaca gaatattagt
    12061 accatttgga aaaggtaata aaccaacaga agcctttgta tttcaattta cagatagctt
    12121 agatgaaaaa ataaaaataa aagaaattat tgatatatta gatgaaaatc caatatttag
    12181 aaaagaagat ttagagttgg tttattggat gaaaaataga tatttgtgta cttatataga
    12241 atgtataaat ttgatatatc caaagggata caaattaaat aattataaag tagtcctatt
    12301 aggtgaaagt ttaagtggat taaatgagat tgaattgaag gaaaaaatat caactttaag
    12361 tgataaaaat agagaaatag tagagaaagt gattgatagt aaaggtaaaa ttaaggttga
    12421 taagttaaaa tatataccaa acttaaatag ttctttgtac acaatgaata aaaatggaat
    12481 tataaaatta tgttgggaat ataaaaatca caaaaatgaa aagaaagtat gttatatatc
    12541 tttgagttta gaaagtgata agattgacga ttatatagag caaaataaaa taaatgtggg
    12601 aagtaaacaa aaagagatat taagttttct taaaaataat gaaaatgtag agataaatga
    12661 tttattggat ttattaaatg cttcaaaaca aagtataaat tctctaagta agaaaaagct
    12721 aataacccta gaatttaagg attattatag agagccaaaa agtatttaca aatccgtact
    12781 aaaaagtata aaattaaaca atgaacaaca agaagcagtt gatgagataa agtcgaatat
    12841 gtttgtagat gataaaaaac catacttaat tcatggagta actggaagtg gaaaaactga
    12901 agtttacatg gaaataatag aatttgctct aaatcaaggc ttagacagta tattcttagt
    12961 acctgagata gcattaactc ctcaaacaat agataggcta aagagtaggt ttggagattt
    13021 agttggagtg ttccacagta aactatctga aggagaaaaa catgatgttt acaaagctgt
    13081 caaagcaggt aaagtaagag tgttaattgg agctaggtca gctttatttg caccttttaa
    13141 ttccttaggc ttaataataa ttgatgaatg tcatgagtca tcatataagt ctgagaagaa
    13201 tccaaaattt aatgctatag aagttgctag atttatggca ttaaaaaata atataactct
    13261 tattttaggt tcagcaacgc cctcaattga agagtattat agggcaaaat ctggagaata
    13321 caaacttata aatataaaaa gtagagcaaa tgataagcct cttccaaaca ttgaagtggt
    13381 agacatgaaa gatgaattag ataaaggaaa tagaagtata tttagtttga aattacaaaa
    13441 agaaatacgt tatgcgattg aagaaaataa tcaagtgata ctatttttaa acagaagagg
    13501 ttatgcaaat tttgtatctt gtagaaagtg tggatatgtg tttcaatgtg aaaattgtga
    13561 tatatcttta acttatcata agaaaagcaa tactggaaga tgccactatt gtggatatga
    13621 gaaagaaatt ccaaaagagt gtcctgaatg taaaagtact tatgttaagc catttggagt
    13681 tggaactcaa aaaattgaag aggaattaaa atatattttt ccagatataa aaactttgag
    13741 aatggataaa gatacaactt caaaaaaagg tgctttagat gagatactaa ataaatttaa
    13801 agataaagaa gcagatgtac ttattggaac acagatgtta agtaaagggc tggattttga
    13861 gaatgtaact ttagtaggta ttttgtctgc agacatgata ttaaattttc cagactttaa
    13921 aagttttgaa actacatttc aattgataac tcaagtctct ggaagagcag ggagagctga
    13981 taaagaggga aaagtagtac ttcagactta tgacactgaa cactatgcta ttaagcatgc
    14041 tattgaatat gattatgagg gtttttatga agatgagata aaaataagaa aagcatttgg
    14101 atattctcct tttaataata tgttaagtgt agttgttagt ggtgaagatg aaagattagt
    14161 gataaaaaat ataaaaaata tgcatgcatc attgatttat ttattagaaa aaaggggtat
    14221 aaatgattta ggatttatat taggtccaaa tccatgttca atatcaaaaa taaatcaaaa
    14281 ttatagatgg caaatactct ttaaagatga aaatattgaa attaatctct taaagggtat
    14341 aataaaatat atatgtataa caaaacgaga tttgatattt gataagaata tcaatgtatc
    14401 tatagatatt aatccaaaca gtgtattata aatttaggag gaattataat ggctttaaga
    14461 caaatagttc aaataggaga accagtatta agaaaaaaat caaaaaaggt tgaaaaaatt
    14521 gatgaaaaga taatacaatt attagatgat atggcagaaa ctatgtatga tgcagatggt
    14581 gttggactgg ctgcacctca agttggaata ttaaaaagag tagtagtaat agatattgga
    14641 gaagagctta tagaacttat aaatcctgaa ataatagaaa catctgggga gcaaatagat
    14701 gaggaaggat gtttaagtgt tgttggagaa gctggaaatg ttagaagacc aaattatgta
    14761 aaggtcagag ctttaaatag aaatggcgaa acaatagagt tagagggaga agaactttta
    14821 gctagagcat tttgtcatga gatagaccac ttagatggaa ttttatttgt tgacaaaata
    14881 gaaaagtagt aggagttgat ttgatgaaaa tagtatttat gggaactcct gatatagcag
    14941 ttccatgttt acaaaaaata atagatgaga agtatgaaat attgggtgtt gtaactcaac
    15001 cagataaacc aaaaggaaga ggtaaaaaac taggtatgag tccagtaaaa gaactggcaa
    15061 ttgaaaataa tatacctgta tatcaaccag taaaggctag agataaagaa tttatagata
    15121 aaattaaatc tttaaatcca gatgtaatag tagttgtagc ttttggacag atacttccaa
    15181 aagaaatatt agagattcct aaacttggat gtataaatgt tcatgtttct ttacttccaa
    15241 aatatagagg tgcggcacct ataaattggg taataataaa tggtgaagaa aagactggtg
    15301 ttacaactat gtatatggat gaagggttag atactggaga tatgatacta aagacagaag
    15361 taaatcttga tgaaaatata acagcaggag aacttcatga taaaatgatg aatataggag
    15421 cagaaacttt aaaagaaaca ttaagattaa ttgaagaagg aaatgctcca agagaagtac
    15481 aaaatcatga agaattttcc tatgcgccaa taatgaataa atcattaggt aacatagatt
    15541 tttcaaaaag tgccagagaa atacacaatt tagttagagg agtaaatcca tggccaagtg
    15601 cttatactat ctataatgat gtcataatga aagtttggaa aactaaagtg ttagatgaaa
    15661 aaagcacaaa agatgtaggt acaataattg atgtaagcaa agatgggata aaagtaagta
    15721 ctatagataa tgtactttta attgaagaaa ttcagatgcc gaataaaaag agaatgttgg
    15781 ttggtgaata tataaaaggg aatactatag aaactggttt agtattgggt taggtgaaaa
    15841 gtatgacaga tataaagaag tatttaacaa gtgttgggat tttgatttta atattaggaa
    15901 ttttgatagg agttgttaat ttttttataa taagtttaac tcaaatattc tccttgatga
    15961 taaatattat aataatatca ctaatactta taatactatg ttcaacagca gttacttata
    16021 gagtaattaa aggaaagtat gttaactcaa atataatgaa aataaatttt aatattgtca
    16081 gtggattatt tcctattata tctttcattg catcatcatt tggtatgtca aaaagtgata
    16141 ttagaagaat atatataaag ttaaataatg aatatatata cagtaataaa tataatttta
    16201 atccagagga tataataatt ttaatacctc attgtattca agagaatagt tgtaaattaa
    16261 aagttactaa tgatatagat aattgtaaag aatgtggaag atgcaacgta ggagaattaa
    16321 ttaagctaaa ggaaaaaact aatgtaaaaa tatttgtagc tacaggagga accctagcta
    16381 gaaaaattat aatggataat aaacctaaag ctgttgtagc tgttgcatgt gaaagagatt
    16441 tgacatctgg aatacaagat attaagaaaa ttcctgtttt aggtgtattt aacaaaagac
    16501 ctaatggacc ttgtgtaaat actaatgtag acatgatgga tattgaaaaa gctatagact
    16561 ttttaacagg aaaaaatata ttaatttgta attaaactgt ttttaagtta agtgaatatt
    16621 aagaggatgt ataataaaaa tatattttag gagagtgtta tttatgtacc ctgtatatgg
    16681 cggtttttgg ggatttgacc caacaatggt tgtattaatt ccagctatat tacttactat
    16741 atatgcacaa tttaaagtga gttcaacaac aaataagtat ttaagagtaa atacacgtcg
    16801 aggatatact ggagagcaaa cagcaagaag agtacttgat tcaaatggtt tgtatgatgt
    16861 aaagatagaa atggttagag ggcatctaag tgaccattat gacccaagaa gaaaggctgt
    16921 aaggttgtca gaagatgtat attatggaac atctataact tctgttgcag tagctgcaca
    16981 tgagtgtggt catgccatac aacatgctaa aggttatgcg cctcttcaaa taagaagtag
    17041 tttagtacca gtagtgaatt ttgcttcaag tatatcttgg tttttaatat ttttaggatt
    17101 tattatggct ggtccatttt taaaaattgg tatactattg ttttcggctt cagtgttatt
    17161 ccaaataata acattgcctg tagaatttaa tgcttcaagt agagcaatcg ttcaacttgg
    17221 gaatttaggt attatagatg aaagtgagtc aagacaaagt agaagagtac tttcagcagc
    17281 agctttaaca tatgttgcag cagcattggt ttcaatactt caattactta gattgttact
    17341 aatagctcaa agaagaaatg actaaaagta aagtgggttg tttagaaaca accctttttt
    17401 atgtttaata aaagggagaa atttatatgg atgcaagaga agttggattt aaagtattat
    17461 gtgatataga aaaaaatgac aattattcaa atattgcaat aaataagcat tttaaaaatt
    17521 tagaaataag tgatatggat agaggacttg caacagagtt aatttatgga gtaatagaaa
    17581 ataaatatta tttagattat ataatcaata aactttcaaa aataaaggta aaaaaaatgt
    17641 caacttatgt taaaatcttt ttaagaatgg gaacatatca gattttattt ttagatagta
    17701 tatcagatta tgcagcagtt aatgaaactg taaaattatc taagaaatat gataaaaaat
    17761 cttctggttt cataaatgct attcttagaa atgaaataag agctaaagag actataatgg
    17821 acataactga ggaagatagt gtaaaatact tatctataaa gtattcctat aactcttgga
    17881 ttataaaaaa ctggatagat aaatttggac aagaatttgc tgaagatttg ttggaagcaa
    17941 ataatgaaaa gcctagtatc tatataagaa cgaatacact taaaataagt agagaagaac
    18001 ttattgaaaa attaagtgaa atgggtattg tatgtttaaa agttcctatg gtagaagaag
    18061 ctattaaagt cgaaaaaatg aaaaatatag aaaaaaatga attatttaag gctggtttat
    18121 ttacaattca agatataagt tctatgatag tagggaaagt aattaatcca aaagaaggtt
    18181 catttgtttt ggatgtatgt agtgctcctg gagggaaatc tactcactta gctactttaa
    18241 tgaacaatac gggtcaagta gtagctagag atatttttga gcataaacta aagcttataa
    18301 actctactgt aaatagattg gggcttaaaa atgtgtttgt agaaagattt gatgcttctg
    18361 agattgatga aaatagtata agtaaatttg attatgtttt agcagatgtt ccttgctctg
    18421 gttttggtat aataagacgt aaaccagaaa ttaagtataa gaaagaagaa gagttaaaag
    18481 acatcacttc tatacaaaag aaaattttag aaaatgcatc taaatatgtc aaagttggtg
    18541 gaactttagt atatagtaca tgtacaattc aagatgtgga gaatataaat atagttactt
    18601 cttttgttga agaaaatcac aactttgaat ttgttccaat tgatacagta aatgtagact
    18661 tagacaatca agataaagga tatttaaaga tatatcctaa tattcatggt atagatggtt
    18721 ttttcatagc aaaattaaag agaataaggt agtgagagaa tggaaaagaa aaagatagta
    18781 ttaaaaaatt ttactgaaga tgaacttaaa gaatttatga aaactataga tgaaaagccc
    18841 tttagaggaa gtcaaatatt ctcttggata tataagggtg caaaaacttt tgatgatatg
    18901 aacaatatac caaaaagttt gagaaataaa ttagaagagg tttcatgtat agggaatata
    18961 gatatagaat taaagttaga atctaaggta gataatacaa aaaaatattt gtttttatta
    19021 gatgatggaa atataataga aactgtgatg atggattatg atagtagagt tactgtctgt
    19081 gtttctaacc aagtaggttg taggatggga tgtaattttt gtgcatctac aatggatgga
    19141 ttaattagaa acttagagcc atgggaaatt ttagaccaag ttattaaaat tcaagaagat
    19201 actggtaaga gggtatcaaa tcttgtattg atggggagtg gtgagccact tgataacttt
    19261 gaaaatacaa aacaattctt aaaaataatt aacgaaaaaa atggtcttaa tatagggtat
    19321 agacatataa ctctttcaac ttgtggaata gtacctaaaa tgtatgagtt ggcagactta
    19381 gaaatagcta taaatttagc tttatcactt cattcaccat atgatgaaga acgaagaaaa
    19441 attatgcctg tagcaaatgc ttattctatt gaagaaatat tgaatgcatg tagatattat
    19501 ataaaaaaga caaatagaag ggttacattt gaatattctc ttataaaagg agtaaatgac
    19561 tcagaaaaag aagcaaaggc attagctaag ctcctaaaag gtatgctatg tcatgtaaat
    19621 ttaattccaa taaataaagt tgaagaaaga gaatacgaaa agccagataa agcatttatt
    19681 tataaattta gggatagttt ggagaagaac aatatacctg ccacagttag gatgtctatg
    19741 ggttctgata ttagtggagc ttgtggtcaa ttgagaagaa aatacaagta atttgtaagg
    19801 aggaagtcgt atggtttata gttgtgcctc ccatatagga aaaataagaa aaaacaatga
    19861 agactattgt gagggagaag ttatagatac agaacatggt ccaataggaa tatttgccat
    19921 agctgatgga atgggtggac ataaaaaagg tgaagtcgcc agtaaattag cagtagaaaa
    19981 tataattgat tttctaaagg aaaacttatt acaacatgat aatgtaaaaa tagactatat
    20041 agatgatata ttaaaacagg cttataataa tgtaaactca attgtgcaca aaaaatctat
    20101 ggaagatata gaatttgaag gaatgggaac tacattagtt acggctatag tgtataataa
    20161 tgtcttatat gtggcaaatg ttggagatag cagatgttat ttgttaacag atgaaaaatt
    20221 tgataaaata actatagacc attccgtggt tgaagaactt atgatggccc atgtcattac
    20281 agaagaagag gcaagaagac atccacagag aaatagaata actagagcaa ttggaacaga
    20341 tgatatggtt gttgtagata tttttaagaa agaaatcaaa aaaagtgata taatacttct
    20401 tgcgacagat ggattaactg ggtttattga tgatgaagat ataagagatt taatattaga
    20461 ttatgaatat gagagaacta gtaatataag tgaagaattg ataagtatgg ccaatgatgt
    20521 ttctggtaaa gataatgttt cggttatagt gataaaagta taattaggac ggtgatattg
    20581 tgggagatac aattttagga aatcgatatg aaatcatcag gaagattggt gatggaggaa
    20641 tggcctttgt atatgaggct aaagacagat tattaaacag gactgttgca cttaaagtac
    20701 ttagacctga atttgtagat gatgatgaat ttctaacaaa gtttaaaaga gaggcagaag
    20761 cagtagcaag tctttcacat ccaaatatag taaatgttta tgatgttggt gaagatggaa
    20821 aagttcacta cattgtaatg gagtttgtag atgggaagaa tctaaaagag attatacaag
    20881 atgaaggtat attagatgaa tatactgcat tagatataac aaaacaaata gctatggcac
    20941 ttagtgcagc gcacaaaaag gggattatac atagagatat aaaacctcat aatattctca
    21001 tatcaaatga aggaagagta gtaaaagtgg ctgattttgg tatagccaaa gctgtttcaa
    21061 attcaacaat gactaatatt ggtagtataa taggttcagt gcattatttt tcaccagagc
    21121 aagctaaagg taaatttgta actaataatg ctgacctgta ttctctaggt atagttttat
    21181 atgaaatgtt gataggaaaa gttccattta ggggagatag tccaatttct atagcacttc
    21241 aacacattaa tgatgatata gactttacat cagaagaaaa agttaggatt cctcaaagtg
    21301 taagaactac aataaaaaag cttacagaaa aatctagcgc agatagatat caaactgctg
    21361 aagaattgat agaagatata gagtatatag aaaaaaatat tgatttagat tttataaaag
    21421 aatatgatga ttttgcaact aagaaaattg atgaaaaaga aattaataaa gttgtaaatc
    21481 caacacttgc aaaaccagct cctgaaaaag tggttaagcc tgttgaagta gctgatttgg
    21541 atgatgatga agattactat gatgattttt atgaagaaga tgaagaagaa gaagaagaag
    21601 aggaagagat aatgagagct aaaaagaatc agagacctaa gagtactcca agtaagagaa
    21661 ctaagaaaaa gaagaaaaaa caggaaagcc caaaatctag aagaagatta aaagtaattg
    21721 cggctgtttt gatactgata ttgtgtgctc aagtattctt agcatataag tttttatttg
    21781 ctggagggtt tggcaataaa tctttgactg ttcctaatct tgtaaatatg acattagaag
    21841 aagctcaaag tgctgttgaa aaagaaggat tatacctgag tgtaaaaagt gaagagtata
    21901 atagtgaagt tgatgaaaat tgtataatat cacagacacc tgaaggtgga agtacaaatg
    21961 tcaaaaaggg tgatactata aatgttgtcg taagtaaagg ttcaagtcag gcatctgttc
    22021 ctaatgtggt gggacttact ttgagtaatg caaaacaact tattgaagaa aataacttaa
    22081 aagtgggaac tgttaagtat gaatatagct caatatataa agaaggtaca gttttaagtc
    22141 agagtccagg tgctggctca tcaagagctc aggaaggtga tgaggtaaac ctatatgtaa
    22201 gcaaaggttc agaaaaatca aatacacaga ctccaacagt accagataaa aaaccaacaa
    22261 caccagagaa taatacacca acagaacctg gttcaaactc agggaacagt ggtggaaatt
    22321 caggaagttc aaactcagga aatagtggag gaaactcagg caataatgga ggtaactcag
    22381 gaggttcaaa ctcaggaaat agtggaggaa gctcaggagg ctcaaactca ggagataatg
    22441 gaggaagttc aggaggttca aactcaggag ataatggagg aagttcagga ggttcaaact
    22501 caggagacaa tggagatagt tcaggaggct caaactcagg agataatgga ggaacttcat
    22561 caggaactgg cgcaaacatt ggtaaaacag aataaatatt aataaaataa gagctgtcct
    22621 tttatagaaa atattttctt taaagggcag tttgttttgt ataaaatact gaattggtta
    22681 taatataagg agaaaagtat ttatgaagaa gaaaaccgtt ttaattacag gaggagctag
    22741 agggattggt aaagctatgt ctaaagcttt tgcaaaagaa ggttacaatg tattagtaaa
    22801 ttttaataag tcagaaaatg aagctaaaga gttgtatact atattaaatg aaaaaaattt
    22861 ttctgtaaaa ctttttaagg ctgatatttc aaatagagaa gatgtagaga atatggtaga
    22921 ttattgtata aaagaatttg gaggattgga tgtacttgta aacaatgctg gtgtaagtca
    22981 agataagttg tttacagata taaccgatga agattgggat aatatgatga atattaattt
    23041 aaaaggaagc ttttattgtt cgcaagttgc tttaaaatac atgatatcag aaaaaaaagg
    23101 aaatataatt aatatatctt caatctgggg tatatcaggt gcgtcttgtg aagtgcatta
    23161 ttctataaca aaagcaggta taataggtat gacaaaggca ttagctaaag aagtaggacc
    23221 atcaaatatt agagtaaata gcatagctcc aggtgtcatt aatactgaca tgttatctgg
    23281 atataatgag gaagatatag acgctcttgt ggaagaaacc cctctaatga ggctaggaac
    23341 tccagaagat atagcaaatt gtgctatttt cttggcatca gacaaatcaa attttatcac
    23401 tggtcaggta attagtccaa acggaggatt tgttatttaa aaaaaatgtg gtattatata
    23461 aaaataggaa agaataacac gatatactat tcataaacag tatgtgttta tataaatttt
    23521 ataaagggag gcatttgatg ctagaaggaa aaattataaa ggggattagt ggattttatt
    23581 atgtagatac ttataatggt atttatgaat gtaaggctag agggatattt agaaagcaga
    23641 aaataacacc tttggttggt gatagagtca aaataagcat tgttgatgaa gatgaaaaaa
    23701 agggtatctt agaagaaatc gacagtagag atacagaact tataagacct cctattgcaa
    23761 atgtagataa agcattaata gtattcgcaa taaaaaatcc taaaccaaac ttatctcttt
    23821 tagatagatt tatagttttg gcagaaaaag aaaatttaga aactgtaata atccttacaa
    23881 aagcagattt agatgataat gatacattag aaactgttaa aaatatatat gaacttagtg
    23941 gctataaagt cattcctgta agcaatatta ctaagttaaa tatagataaa gtaaaagaag
    24001 aattaaaaga aaatgtagta gtgtttgcag gaccatctgg agttggaaaa tcaagcttat
    24061 taaatgaaat agatgaaaat tttaaacttc aaacaggagt ggtaagtgat aaaataaaaa
    24121 ggggaaaaca tactactcgt cacgctgaac ttttaaaatt ggaatttggt ggaatggttg
    24181 cagatacacc aggttttagt tcccttgcac ttgaagatat agaggaagtt gaacttaaag
    24241 attattttat agaatttgat aaatttaatg attgtaagtt tggttcaaag tgtattcatg
    24301 aaaatgaacc taattgtgct ataaaagaag ctgttacaaa tggagaaata tctaaagaaa
    24361 gatatgatag ttatatacag ctgctacatg aaataagaca aaataatagt aggaggtatt
    24421 agaaaaatga ttaaattagc accatcaata ttatcagcag attttgcaaa attattagaa
    24481 gatgtaagaa aagtagaaag tgcaggatgt gaatatcttc atatcgatgt aatggatgga
    24541 cactttgtac caaacattac attaggacca cttgtagtca aaagtttaaa gaaagaaaat
    24601 ataaatatgg tatttgatgc tcatcttatg atagaaaatc cagaccagta tattgaagaa
    24661 tttgtaaaag caggatgtga tataataact gttcaccagg aggcttgtgt tcatctacat
    24721 agaactatac aaaatataaa atcacatgga ataaaagctg gtgtagtact taatccagct
    24781 acaccagtag atacgattaa acatgtacta cctgatttag atatggtatt attaatgtct
    24841 gttaaccctg gatttggagg acaatcattt attccttgtg ttttagataa gattaaagag
    24901 ttaaaagcta ttatagatag tcaaggatta aacattgata tagaagttga tggtggtata
    24961 agccctaaaa acgtagccga agttgtacaa gcaggagcta atgtaatagt agcaggttct
    25021 gcaatatttg gaagtgatga cattcaagaa acagttaatt tatttagaaa gaatgcttct
    25081 cttgaagaat tagtttaaat ttaataatga aaatctgtat tgttttaaat ggcgaaattg
    25141 aagattacga agttacaaga gatattataa ttaaggaatg ttatgactgc ataatatgtg
    25201 cagatggagg ggcaaatcac acttataaaa tggaaataat gccagattat atattaggag
    25261 atttagattc agtagaagaa gaaaagataa acttctataa aaataaaggt gttaaatttg
    25321 aaaaatttcc atctaaaaaa gatgagactg atacagagct ttgtcttttt ttagctaaga
    25381 cacttaaagc aaatcatatt gatttttttg gtgctttagg gggaaggatt gaccacactc
    25441 ttgcaaatat aaagctatta tattatttaa aagaagatgg tatatattct aggatacttt
    25501 ctgataaaga agagatgtat atagttgaaa atgaggaaat ttcattgtat ggaaacccag
    25561 gggacactgt ttctgtaaca gctataaaag gtgatgctaa gggtgtaaca cttactggct
    25621 tagaataccc tctcgataat tattatatga aatactctgt acctattgga atatcaaatg
    25681 ttatgttgag taattcttgt aagattaaag tagagcaagg ctgtgtactt gtagttagaa
    25741 atttataaaa tttgattttg acaattggtt ttatattgta gagcagatac aatcaagtaa
    25801 aatatattta tgattgtatc tgctttttag tatataatta taaatatata ataaactttg
    25861 acataagggg gattttttgt ggtagatagt attatttttg atttagatgg aacattgtgg
    25921 gattcaactg aagaagtatg caaagtctgg caggatgttt taagtaaaca taaggaaata
    25981 gaattaagtg tcacaaaaga tttatttaga agtcttatgg ggctttcttt tgaggaaata
    26041 gcaaatcgtt tatttccaaa tttaagtgaa gaagaaagaa tgaatttatt gaatgaatgt
    26101 tctttgaaag aatgtgacta tttatctgaa aatggagcta aattatttga tgatatagaa
    26161 gatactctta aattactatc taaaaaatat aaattattta tagtaagtaa ttgtcaagct
    26221 ggatatatag aatctttttt aaaagcacat aaactagaac agtattttat agattttgaa
    26281 tgtcctggta atactggttt gcataaagga gaaaataata aacttataat tgaaagaaat
    26341 aaattaataa atccaatcta tgttggtgat acgcaaggtg atgcaaatag tgctaagttt
    26401 gtaggtatac cttttatata tgccaaatat gggtttggga atgtagatgt atatgattat
    26461 tttatagatt catttaaaga tttgttagaa catgatatat taaaataaga tgagtttaaa
    26521 aattatatta aaataataaa aaagttatgg ggggatttaa aaatggaatt acaagatact
    26581 atttttaaaa gacaaagtgt aagaaagttt aaaaatcaag atgtatcaga tgaagatatt
    26641 ttaaaaatga taaaagcagc aggtgcagca ccatcaggca agaatattca aaactggcat
    26701 tttgttgtta taaaacgccg tgatttaatg gaaaagatag cagatgtgat aactaaaaag
    26761 caacaagaaa tacttgtgga aatggataaa gtatcagtgg ataaggctaa tagatttaga
    26821 aaatttgtta aaaactttac actattttac ttaaaagctc cagttttagt attagttttt
    26881 acaaaggtat ataatccatc aggatattat gaattagagc ttatagatgc acctaaggag
    26941 actatagata aattatttat aagaaatcca ggtatgcaga gtctaggtgc ttcaattgaa
    27001 aactttacac tatcagcaat tgaacttgga tatggttctt gttggttgac aagtcaaaac
    27061 tatgcagcag atgagataga agctgtttta gaagcagaaa ctggttttga aaagggagag
    27121 tattttttag gtgcaatgtt agctcttgga gtgccagagg acaatttgaa gagtccatct
    27181 aaaaaaccag tagaagaaat atgtacattt ataaaataaa agtcaaggac attttaaaat
    27241 caaagtaaat taaaaacaag ataaattaaa aata
//
LOCUS       DAMJVX010000034        26145 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMJVX010000034 DAMJVX010000000
VERSION     DAMJVX010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26145
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.036"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     repeat_region   545..901
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=545..573
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            1140..1328
                     /locus_tag="PC407_001375"
     CDS             1140..1328
                     /locus_tag="PC407_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496830.1"
                     /translation="MKIITDSSRADYFKQRRQNKKTFSVLLDREKVEKIEEHLKKQNK
                     TKTIWLEEKINEELEKEE"
     gene            1447..2313
                     /locus_tag="PC407_001376"
     CDS             1447..2313
                     /locus_tag="PC407_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HDF2496831.1"
                     /translation="MKNLIVKEFNGSQIYTFMWKEKSCWIANQIVGLFDYADVSKTIQ
                     DCIKAEDFEIEQEYDVLKGNEFNDFVTTLNVVANNIISNKARSITIFYEDGLYGFLQY
                     TDKPIGVQFRKWLRREVLPSIRQTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLK
                     ELLDGAGFDNKSKLLTAKTLYKKAGIDLPIEINEEEHYFDTKQIASKLKIYSKSNKPA
                     QLAVCEIIKKIDLEENEVKGVWETNGSWTGTVNKYTKSVIDKVRNWIEENNRPTKIAG
                     EKKNYHVVYKIE"
     gene            3178..3705
                     /locus_tag="PC407_001377"
     CDS             3178..3705
                     /locus_tag="PC407_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDF2496832.1"
                     /translation="MREGKRRRGCLFWFILIILFSGVVGAIAGNSTNNESTEKQKEDL
                     TKYIGEEGSIGDLKLTVNSISKASEISVASGYLAYTPDSGKYGIINLTIKNQTKESQS
                     FMLNSFTLIGPDDSKYVPSLLIDVGNKYITMDTVNPNLDVTGNIAFEIPKDLLVSDCK
                     LKYSGSEQEFILKEK"
     gene            3843..4610
                     /locus_tag="PC407_001378"
     CDS             3843..4610
                     /locus_tag="PC407_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HDF2496833.1"
                     /translation="MGLFGKKKADICCICNTEIGVLNIEDGWICNSCFKEYCDALSMT
                     KAPKILRKLDIEKTISSTKKNNELQKIFNTTNNIENYIEFDEDNKKWLVSKKSINDKK
                     TPIIHSYEDIVKFELLENGETVTKGGIGRALAGGILFGEAGAIVGGITGKKTTRKVVD
                     TFKIKITINNIDNPIEYIELINKKTKTNSSAYEKAYKDAHKILSTLSAITQSIKETDN
                     INTKSVADEILKYKNLLDMEAITQDEFNTKKKELLNL"
     gene            4676..7048
                     /locus_tag="PC407_001379"
     CDS             4676..7048
                     /locus_tag="PC407_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDF2496834.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTTSINTTINSLERLQQRLHNPLN
                     TGGIQASQQSLNNIENILTRIEQKIGRNTNEQENFNNKIKQGSEAGSLLVSKLKSLAG
                     IYIGIRGIESITKAADTIASTKARLNLMNDGLQTTEQLNKMIYLSAQSARASYADTAA
                     QVAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMPVTFSQALTMMKNDAYMIFGQTLGKISGALQSVRFSEIVVSIRNVMIAISSNIYDT
                     LNIIKNILNSEFFSNLVQGFTFCAVVITKSLGSIVNTALNIVNIFAQNWSIIKPIVFG
                     VASVFILFRGVLLATKIATIGNVIANVAHAASSSLSALMTNIQAAALMRSNGATLSAT
                     IATWGLNAALLACPITWVVLGILAFVVVVFVAVAAVNKFAGTSLTALGVIVGAVFAAV
                     AAIQNVMIWLLNGCIAVNEGITNGWNQCVYLMKQAIAKGVIFIIEKMASLNNSVNNAG
                     NALGKAFIDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYIKQQIDSL
                     NRWVGDAPEKVKLERMGYKDIGAAFQKGNALGTKWQNAITDKFKDTFDINKMLEDAKK
                     KLGLDDLWNKQNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQ
                     ETINRFTGVNIKIDMNNTNNISKDTDVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            7065..7754
                     /locus_tag="PC407_001380"
     CDS             7065..7754
                     /locus_tag="PC407_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2496835.1"
                     /translation="MAYDFYLDGVQLPIAPPKLEVKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDADNGSDTKVAIKLKQYRDYSTKKLVLAPPKNETGRPNVKIEPK
                     RVDSVNATNTKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKNLSKY
                     TIYKGQVLKLG"
     gene            7747..9639
                     /locus_tag="PC407_001381"
     CDS             7747..9639
                     /locus_tag="PC407_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2496836.1"
                     /translation="MVDELVLANDRDVRLVIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKILAKDFNLKYNVIEDTKYKISRVEENKTLFDMILTALDDTLRE
                     KKEMYTLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSAHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     ALGDIRVRAGCLVPVFLNLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSIMKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLNKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSNTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            9653..9913
                     /locus_tag="PC407_001382"
     CDS             9653..9913
                     /locus_tag="PC407_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HDF2496837.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESISPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKMGDKLVLIRMQGGQQYLILDRM"
     gene            9918..10337
                     /locus_tag="PC407_001383"
     CDS             9918..10337
                     /locus_tag="PC407_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2496838.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLHIEKSKIDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENIDNFEFQNIKGKVQCRFSVHTKYGNIKAEKVVSV"
     gene            10338..11387
                     /locus_tag="PC407_001384"
     CDS             10338..11387
                     /locus_tag="PC407_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2496839.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLEATHTIAKGVFNIDIPLDSRFNLGE
                     YNYVAIERISEKTYKMKCETTGPVFELGKLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYRDEVNKIQDVGGVKVYPTWDGGGTVKLVIINSNFKV
                     PSEELVNLVQEEIDPIGHQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINDVKSNL
                     TIDSNSIVVRGEVVG"
     gene            11380..11997
                     /locus_tag="PC407_001385"
     CDS             11380..11997
                     /locus_tag="PC407_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDF2496840.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            12009..13034
                     /locus_tag="PC407_001386"
     CDS             12009..13034
                     /locus_tag="PC407_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2496841.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTELKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNINNIELKIDPTIIFATLKDIQDLDAK
                     ISNVNTKIDTTKTELTSNIETAKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTIT
                     IDDWIDNQEGGFKATVTHGLLTQRITVSIIDATTKDNVVPDFTIIDDNSIEVRSGVKV
                     ELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIHEEIGKVAEQLTGINSNIISTV
                     NNDILPI"
     gene            13051..14577
                     /locus_tag="PC407_001387"
     CDS             13051..14577
                     /locus_tag="PC407_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496842.1"
                     /translation="MALSMSYFNLPDKRKYTKNLAFNPFAGGRQNFEWTGGDHGLNGE
                     FKETCLSCTYNGSTLNWGSGNVWVLGEYGQYTFTYNCESMHVDTQQKFPYTSNRIITI
                     KGRPVISGSDTSLGNKRKGFSVDFTVSDDTPNVNLIVRAYLDDKLIQNITPVVQNSTL
                     TATVTDSQLNSLSVDGNHKLKIQLNDGYDNFDRIFTFKKIEKGIDISTSLVTDSQAKF
                     TVTKIYSELTKIECYLDETLKETFTTDLYSEKTINYELIDNAIHTLKIVVTDAENVVE
                     EKVISISKNIMPLQPDATLQDISTKLTEIGQGVRNGKTSIINTLALKNIDASLNNTLV
                     ELSEKIKGGFDSGDASLQDLMNQLTQANNTISQLNTKYKVASGTVTSFADSTKIAYPY
                     LTDNVTKPGSWIKVSNLGFKPNIFFADFDYYDAEYKNNYKLFLFACNGVATQRGVDFS
                     SVTSFIRKSGDEYFHANGWLYSNSEGDVYFNNTGVQIPAYNFDSTQKHTYKWYAIKFI
                     "
     gene            14592..14885
                     /locus_tag="PC407_001388"
     CDS             14592..14885
                     /locus_tag="PC407_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496843.1"
                     /translation="MNVPNRIIYDQTGRTIFETGESCGDVLPHYTITELHYIDIEYGS
                     IDYTRNRVIGINIETKEPILEEIPVYITDEEKRIQELENQLLIAENEKVGGLL"
     gene            14885..15067
                     /locus_tag="PC407_001389"
     CDS             14885..15067
                     /locus_tag="PC407_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496844.1"
                     /translation="MNINNVVVRILAERILSKGLNPLKNREFQLDDVTNTEYRKAVED
                     YIIKNSGVVEGAEPTI"
     gene            15101..15667
                     /locus_tag="PC407_001390"
     CDS             15101..15667
                     /locus_tag="PC407_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496845.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTESKFTSENVEGALKECIDR
                     ADEAFQEADSGKTLLSTAIGSPTTSEQTFQDYANYITGFKSNISNLETQLKSKYSIRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITINS
                     NKTGFSVHSYDTSYESYPFTGYYIACFA"
     gene            15698..15988
                     /locus_tag="PC407_001391"
     CDS             15698..15988
                     /locus_tag="PC407_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496846.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNNSNRKIQDETETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKIEASTKQAHLRIDEIVNRIGGK"
     gene            15992..16249
                     /locus_tag="PC407_001392"
     CDS             15992..16249
                     /locus_tag="PC407_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2496847.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQLKDGEK"
     gene            16510..17373
                     /locus_tag="PC407_001393"
     CDS             16510..17373
                     /locus_tag="PC407_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDF2496848.1"
                     /translation="MVEVKEEKTFDEQIDILKSRGLIINDKEDAKFVLSNVNYYRFTA
                     YLLSFKNDDGSYKEGTTFEEVYDIYRFNKEFRILLTDLLGSIEIAFRTYIAYTLAIKH
                     GACGYLERESFKDEKFYINFLTALEREKSNNSDKLFIIHHKEKYEGKLPIWVATEIMT
                     FGMLSKLYSNMLPEDTRYIKNNLCRVNTLLVKSWLQSLTQVRNQCAHYGRIYNNNFRI
                     ITIKNEYKKYNLDNKKIFSYILAMKHLTMDKLIWNSFFIKLQKLINDYNNSIDLKLIG
                     FPNNWIEILAK"
     gene            17452..18264
                     /locus_tag="PC407_001394"
     CDS             17452..18264
                     /locus_tag="PC407_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2496849.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            18358..20757
                     /locus_tag="PC407_001395"
     CDS             18358..20757
                     /locus_tag="PC407_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2496850.1"
                     /translation="MIFKKYIKSLLIVVSVTLIMNKSILFVNALDLESNNNIKSYKRK
                     NVKEIDISNKFVDESFRRDIVEKVLDNRDDPLTNEVASKDNYKIYESDLNKIESCLEL
                     DLHKNPDVSNPNKIDNLDGIENFRNLEKLDCSSNNLESVDLSKNIKLKYLDISGNKIL
                     NIDLKNNNLINELHCDANKLSNLDLKNNKNLVSLFFTSNELTEIDLSNNVNLENLQFL
                     NNNLKSIDLSHNKKLKSLNCTNNKLKSLDLSNNVFLKNLSCSGNNLSKLNIKGLTSLE
                     YLGCAKNKLQDLDVSTNIKLDSLLCGKNSLTKIDISKNKELNSLECSGQYIEKSIIKN
                     GENIVVDIGIENSKLKELYPQNYNISTGFLTWENLSDFPNSISYSLEYEYGPVNDTET
                     MDVNISIDKLVKCRIRYIDYDGSIINEKEISDNSSINEIQINPQRENYEFSGWRNNKD
                     EKIYTSEQVSKLVFDDSTDFIATYKRKSTGSSGSSGGTSSKEDFNIAPSKEKITGADR
                     NETSVKISQKGWNKADNIVLINDSSISDALSATPFAKSKDAPILLTKNNNLNKLTEKE
                     INRLEAKNVYIVGGLKSVDEKVVSDLKKKGLNVIRISGNDRYETSIKLAKELDKNSNL
                     SKVVVVNGEKGLADAVSMGAISAKEEMPILLTNQNDDMKDIKDLIANKNISKSYVIGG
                     ESLFNNKEVNNTLPSVTKIAGSDRTETNSKVISHFYSKDTLNDLYVAKNGMNKQDDLV
                     DALSVGVLAGKTESPVVLVGNGLDDSQKELIKNKKFKNITQIGGNGNEKAFNEVENLV
                     K"
     gene            complement(20798..20983)
                     /locus_tag="PC407_001396"
     CDS             complement(20798..20983)
                     /locus_tag="PC407_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDF2496851.1"
                     /translation="MILLEQRKLRIGFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            21678..22355
                     /locus_tag="PC407_001397"
     CDS             21678..22355
                     /locus_tag="PC407_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2496852.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSRLYFDLSTPGPNHNTKIDINPPS
                     FEKPLKK"
     gene            22529..24565
                     /locus_tag="PC407_001398"
     CDS             22529..24565
                     /locus_tag="PC407_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HDF2496853.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLKNVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(24756..25322)
                     /locus_tag="PC407_001399"
     CDS             complement(24756..25322)
                     /locus_tag="PC407_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2496854.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKND"
     gene            25606..26133
                     /locus_tag="PC407_001400"
                     /pseudo
     CDS             25606..26133
                     /locus_tag="PC407_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
BASE COUNT        10552 a         2850 c         4601 g         8142 t
ORIGIN      
        1 tgaatttcat tgtttatata aaaaaaatgg aaaaatatgt aataattata ggttataata
       61 attgtagcaa ggataataat cgaaagtgcg aagggtgatt attttcatat taaacgccaa
      121 attccaaata aggaaggagg tgaaattata tgataggttt tttattaagc atactagctg
      181 gtgttatatc agcttatatt tatgacaaaa taaaaaatca cccagacgcc aataagggtg
      241 atttaaaaaa ataatatttt cacttaacaa ctgaaaataa tcactctttg taggaataaa
      301 ttatttcctt gcttttatta taccacaaat tggtacagat attcaaaaat aatattttta
      361 tgatataata aaaatgtaga gattttgcag tgagcgatat ttgtgataaa ttgaagttta
      421 acagttgcaa tataaggcgt tgagggtgtg tgataaatgt tatcaattgc actactcatg
      481 gttcactgca aatttaagag agttgtatat gtgtaggtat tgaaaatact caacttattt
      541 tggggtttta gattaactat atggaatgta aatgttgtaa gaagtatcat tctatttttt
      601 aatctttctg ttttagatta actatatgga atgtaaattt cagtgagaat aagctttatt
      661 gtcgatgtaa cactcgtttt agattaacta tatggaatgt aaatagtaca tataatgagt
      721 ctttaacatc agttatgaaa ggttttagat taactatatg gaatgtaaat gattgtactt
      781 tagcgtctgc actagctttg tctatcgttt tagattaact atatggaatg taaattattt
      841 tacagatgaa caattacagt tacttcttga atgttttata ttaactatgt ggattcaaaa
      901 ttaaatagaa aaaagaaagc acttacttaa atagtaggtg cttttgtttt gctcaaatta
      961 cttaattata aagttgctaa gtacaacttt ttagaattat acaatataaa aatgcactaa
     1021 agtgattgat tttgctcaag ttggtcggtt gagtaaaata attagaaaaa aatgttaaaa
     1081 aactcttgaa aagtgtcgcg atacaatgta taattatatt atcgcgatac agaaaagagg
     1141 tgaaaattat tactgatagc agtagagcag attacttcaa gcagagacga cagaataaaa
     1201 aaacttttag tgttctacta gatagagaga aagtagagaa aattgaagaa catttaaaaa
     1261 agcagaacaa gactaaaact atttggcttg aagaaaagat taatgaagag ctagaaaaag
     1321 aggaataaaa aataagagac gttctccccg accaaagatt gaacatccct tattgacgta
     1381 tattatatac actaactata gtatacgtca ttccttgaaa aaattcaatt aaggagtgta
     1441 atagttatga aaaatttaat agtaaaagag ttcaatggaa gtcaaattta tacttttatg
     1501 tggaaagaaa aatcttgttg gatagctaat caaatagttg gattattcga ttatgctgat
     1561 gtatctaaaa caatacagga ttgcataaaa gcagaagact ttgagattga acaagagtat
     1621 gatgtattga aaggaaatga atttaatgat tttgtaacta ctttaaatgt agtcgcaaat
     1681 aatataatta gtaataaagc tagaagtata actatttttt atgaagatgg tttatatgga
     1741 tttttacaat acacagacaa acctattggt gtacagttta ggaaatggct tagacgagaa
     1801 gttttaccaa gcataagaca aactggtgca tacataacta acaatgctaa tcccgaaaaa
     1861 ctaagagaaa aagcaagtga gattgagaaa ttacagttag cttacaatag tacatctatg
     1921 ttaaaagaac tattagatgg tgcaggcttt gacaataaat ccaaactatt aacagctaaa
     1981 acattatata agaaagcagg aattgattta ccaatagaga taaacgaaga agaacattat
     2041 tttgatacaa agcaaatagc atctaaactg aaaatatatt ctaagagtaa taaaccagca
     2101 cagttggctg tttgtgaaat tattaaaaag attgatttag aagaaaacga agtcaaaggc
     2161 gtttgggaaa ctaatggttc ttggactggt actgtaaata aatatacaaa gagtgtaata
     2221 gataaggtta gaaattggat agaggaaaat aatagaccta ctaagattgc aggtgaaaag
     2281 aagaattatc atgtggttta taagattgag taaatttata agattgtatt aaataattta
     2341 gtttagtttt ggggggatta atacaatgca tgagaattta cttgatagta tagatattga
     2401 aaagagaaaa gaagaattta gaattaaact tttgaaaata agagaaacag atatagatat
     2461 atataataag atagaaagta tagtatataa actttcttag aaaaaattag agaaaaataa
     2521 ttaaataaat agataaagca cttggatatt tcactgtttc aagtgcttta tatggtaaaa
     2581 aatggtataa taaaatatag gaattttgca gtgttcgatt tttctactca agtatagttt
     2641 aacaattgga atacaaggca ttgagaaggt ttgataagtg ttatcaattg cactattacc
     2701 cgctcactgc aaatttgaga aagttatatg tgtgtaagtc ttggaaatac acaatttatt
     2761 ttggggtttt atattaacta tatggaatgt aaatctttgt aatggtagtg tatttaagat
     2821 tgaaacatca agttttatat taactatatg gaatgtaaat caacattagt agttgtcttt
     2881 atacacatag catcacgttt tatattaact atatggaatg taaatacagc tcccaagaca
     2941 tacaacgaat ctgtaacatc agtgttttat attaactatg tggtatgtaa aagacttatt
     3001 tacagcttta tttgctaaat cagaaggttt tatattaact atgtggactt aaaattaaaa
     3061 ataattaaaa aacacttact aatgtaggtg tttttttatt gaaaggaagt gattataatg
     3121 taaaaatttt gaagatatag tataatattc ttataaaata taatttaggg ggatattatg
     3181 agagaaggaa agagaagaag aggatgtcta ttttggttta ttcttattat tttattttct
     3241 ggagttgttg gagcaatagc agggaacagt actaataatg aaagcactga aaaacagaaa
     3301 gaggatttaa ctaaatatat tggtgaagaa ggtagtatag gagatttaaa attaactgtt
     3361 aatagcattt caaaagctag tgaaatatca gtagcatctg gttatttagc atacactcca
     3421 gatagtggca aatatggtat tataaattta acaataaaaa atcaaactaa ggaaagccaa
     3481 tcttttatgc taaattcatt cacattaata ggaccagatg attcaaaata tgttccatct
     3541 ttattgattg atgtaggaaa taaatatata actatggata ctgtaaatcc taatttagat
     3601 gtaacaggta atattgcatt tgaaattcct aaggatttac tagtttctga ttgtaaacta
     3661 aaatatagtg gttcagagca agaatttata ttaaaagaaa aataatatat agatttaaaa
     3721 atatgaagca tttactttaa ataagtaagt gcttttttaa ttaaaaggag gtgataataa
     3781 tgtaaaaatt ttacttatat agtataatta cattataaaa ttattgtact ggggggaatc
     3841 ttatggggtt atttggaaag aaaaaagcag atatatgttg tatttgtaat actgagatag
     3901 gagtactgaa tattgaagat ggttggatat gcaattcttg ttttaaagaa tattgtgatg
     3961 cactttctat gactaaagca cctaaaattt taagaaaatt agatattgaa aagactatat
     4021 catcaactaa aaaaaataat gaacttcaaa aaatatttaa tacaactaat aatatagaaa
     4081 actatataga atttgatgaa gataataaaa aatggcttgt gtctaaaaaa agtataaacg
     4141 ataaaaaaac tcctatcatt cattcttatg aagatatcgt gaaatttgaa cttcttgaaa
     4201 atggtgaaac tgtaactaaa ggaggaatag gaagagcttt agcgggagga attttatttg
     4261 gagaagcagg agctatagtt ggagggataa caggtaaaaa aacaacaaga aaagttgttg
     4321 atacatttaa aattaaaata acaattaata atattgataa tcctattgag tatatcgaat
     4381 taattaataa aaaaacaaaa actaattcta gtgcttatga gaaagcttac aaagatgccc
     4441 ataaaatttt atcaacatta tctgctatta cacaaagcat taaagagaca gacaatataa
     4501 atactaaatc tgtagcagat gagatattaa aatataagaa tcttttggat atggaagcta
     4561 ttacacaaga tgaatttaat actaagaaaa aagaattatt gaatttgtaa tataataaac
     4621 acttactaat gtaggtgttt ttttatatgg aaatttatga aaggagagtg agaaagtggc
     4681 aacgatacaa acttcaataa agattttcga cggaatgaca cctgctttta ggaatatgac
     4741 tacatctatt aatacgacaa ttaatagcct tgaaaggtta caacaaagat tgcataatcc
     4801 gcttaacaca ggtgggatac aagcatctca acaaagttta aacaatattg aaaacatatt
     4861 aaccaggata gaacagaaaa ttggaagaaa tacaaatgaa caagaaaact ttaataataa
     4921 gataaagcaa ggaagtgaag caggttctct attagtatct aaactcaaaa gtttggctgg
     4981 catatatatt ggaataagag gtatagaaag tattacaaaa gcagcagata caattgcaag
     5041 tacaaaagca cgtttaaatt taatgaatga tggattacag acaacagaac aacttaataa
     5101 gatgatttat ttatctgctc aaagtgcaag agctagttat gcagatacgg cagcacaagt
     5161 cgctaaactt ggaatattag caggagatgc ttttggaagt tcggcagagg tgataaagtt
     5221 tacagaactt atgaataaag cttttgtaat tggaggaaca tcagcaaatg aagctagtgc
     5281 ggctatgtat cagcttactc aagctatggg tgcagggaaa cttcaaggtg atgaatttcg
     5341 ctctataatg gaaaatgcac ctttattagc cactaaaata gctgatgcaa tgggaaaaac
     5401 taaagaccaa ttaaaggaat tgtcaagtag tggagcaata acagcagatg ttataagaaa
     5461 tgcactattt aaagcttctg atgagataga aaagaaattc gcaagtatgc cagtcacttt
     5521 tagtcaagca cttactatga tgaaaaatga cgcctacatg atttttgggc aaaccctcgg
     5581 aaagataagt ggagcattgc aaagcgttag atttagtgaa attgttgtat ctataagaaa
     5641 tgttatgatt gcaatatctt caaacattta cgatacatta aatattataa aaaatatttt
     5701 aaatagtgaa ttcttttcaa acttagtgca aggatttaca ttttgtgcgg tagtaataac
     5761 taaaagctta ggttctattg taaatactgc attaaatata gttaatatat ttgcacaaaa
     5821 ttggagtata ataaaaccta ttgtatttgg tgtagcttct gtttttatat tgtttagagg
     5881 ggttttgcta gcgactaaaa tagctacaat aggtaacgta attgcgaatg ttgctcacgc
     5941 tgcttcctct tcactatcag cacttatgac taatatacaa gcagcagcac tgatgcgttc
     6001 gaatggagct actttatcag caacaatagc cacttggggt ttaaatgcag ctttattagc
     6061 ttgtccaatc acatgggtag tattaggtat tctagcattt gtagtagtag tttttgttgc
     6121 agtagcagca gtaaacaaat tcgcaggtac aagtttaact gccttaggag taattgtggg
     6181 tgctgtattt gcagcagtgg cagcaataca aaatgttatg atttggttat taaatggctg
     6241 tatagctgta aatgaaggca ttacaaatgg ttggaatcaa tgtgtatatc taatgaaaca
     6301 agctattgca aaaggtgtaa tctttataat cgagaaaatg gcatctctaa ataattctgt
     6361 aaataatgct ggaaatgcac ttgggaaagc tttcatagat ggggcaaata tcgcaatacg
     6421 aggtgtaaac aaattaattg acctaataaa taaaatacca gggataaata ttggtaaagt
     6481 aggagaggca acgtttacgc cagttaaggc agataatagt tacatcaaac aacagattga
     6541 tagcttaaac agatgggtag gagacgcacc agagaaagta aaattggagc gaatgggata
     6601 caaagatatt ggagcagcat ttcaaaaagg aaatgcactt ggaactaaat ggcaaaatgc
     6661 tataactgat aaatttaaag atacttttga cattaataag atgctagaag atgcaaagaa
     6721 aaaattagga ttagacgatt tgtggaataa acaaaatcct ttaaacaacc ttggtggatt
     6781 tggtggagat ttaggaaaaa atgtaaagga cacggcaggc aatactgcaa agatggcaaa
     6841 gactatggat aaaagtcaag aggacttaaa atacttaaga gatatagcag agcaagaaac
     6901 aatcaatcga ttcacaggag taaacattaa aatagatatg aacaatacaa acaacataag
     6961 taaagataca gatgttgatg gaatagttaa tgtcttaact gaaaaactga acgatgctat
     7021 ggttgtatca gcggaaggaa tagtttagga aggagagtga gaaaatggct tatgattttt
     7081 acctagatgg agtacaacta ccaatcgcac cacccaagct tgaagtcaaa gtgacaaaca
     7141 agaacaagac agttgatttg ataaatactg gagaagtaaa catattaaaa aaagaaggat
     7201 tatctgaaat aagttttgaa gcagaattta cacataataa actacctttt tgtagaggtc
     7261 aatttagaga tgttcaattc tttttaagta agctagaatt actaaaaact gattgtaagc
     7321 catttcaatt tattgtatct cgtgagttag gtaacaaggt cttatttaac actaatataa
     7381 aagtatctct tgaagagtat gctatttcag aagatgcaga taatggctca gatacaaaag
     7441 ttgcaataaa attaaagcaa tatagagatt actcaactaa aaagttagtt cttgcccctc
     7501 ctaaaaatga gactggtaga cctaatgtaa agatagagcc aaaacgagtt gattcagtca
     7561 atgccacaaa cactaaaaca tatacagtaa aagcagggga tagcctttgg tcaatttgtc
     7621 agaaacaact tggtaatggt tcattatata agaaagtata cgaactaaat aaatctatga
     7681 tggataaggc aaataagggc aaaaacttaa gtaaatacac tatttataaa gggcaggtgt
     7741 taaaacttgg ttgatgaatt agtgttagca aatgatagag atgtaagatt agtaatagct
     7801 cattgggaag atttctacga acctgctgtc attgatggta tcacatggga gatagaaaga
     7861 cgaggtacac cttctaagtt agagtttaca atagtcatgg atgatatact agagttttgc
     7921 gaaggaaatt ctgtaaggct gtattataaa ggaataggca tattttatgg atatatattt
     7981 caaaagaaaa gagataaaga aaatcacatt aaaattgttg cttacgacca gttgagatat
     8041 tttaagaata aagatactta tgtatatagt aataaaactg caagtgaact tgtaaaaatc
     8101 ttggctaaag attttaattt aaaatacaat gtcatagaag atactaagta taaaatatct
     8161 agagtcgaag aaaataaaac actctttgac atgatactaa cagcactaga tgatacttta
     8221 agagagaaaa aagaaatgta taccttgtat gatgattttg gaagaataac attaaagaat
     8281 gttgcatcaa tgaaactaga tactgtcatg aacaatgatg taattgagga ttttgactat
     8341 aattcatcaa tagatagtga tacttacaca aagattaaac ttgtaagaga caacgaggag
     8401 tcaggaaaaa gggatgtata tattgctcaa gactcagctc acatgagaag ttggggaata
     8461 cttcaaatgt ttgatacagt agacaaaaac atgagtgaag cagagataaa acaaaagtgt
     8521 gatatacttc taaaactata taataagaaa actaagtcat taagtttaaa aaatgcactt
     8581 ggagatatta gagtgagagc aggttgttta gtacctgttt ttttaaatct aggagatatt
     8641 gaattgcaaa attatatgtt agttgagaaa gtaaaacata catttgaaaa taattcacat
     8701 ttcatggatt tgaccttggt tgatggagac gaatttgctt catattcttc aagctcatat
     8761 agtagtggaa atactaataa taaagatgaa aagaaaaatg gtcctgcaca aagtattatg
     8821 aaaaaaaata caggtaaaaa agttcctgct atatttactg catattatcc aggaaacaat
     8881 gcaatggaag gtggaaaaac agattgcaat ggaaagccac ttgatgtaaa atcaagaact
     8941 gttgctggtc caatgaatcg agaaggagtt aagaaaactt ggtatactga tgattttcta
     9001 aataaacatc cagtttttga atatggagat aaagtaaaaa ttatacttcc tggtactgcc
     9061 tatgacaaca aagtatatac agttaaagat aatggaggaa gaatatatgt tgaaacaaac
     9121 ggaacatatc atatagatat actattagct aatgctagtg aatgtaaaaa atttggtaga
     9181 aagaatggct atataattat aggtggagat gaagaacaaa catatcaagt tgaaggtaat
     9241 aaccaaagta atacaaataa caattctaaa gaagataaat taattagtat agcaaaaagt
     9301 aaactgggtt gtaattatgt gtatggagca gaaggtccta ataattttga ttgcagtggg
     9361 tttactcaat ggtgttataa acaaataggt ataaaaattc ctcgtactgc ttctgcacaa
     9421 agtaaagcag gaaaagcagt agatttaaaa gatagaagca agtggaaagc aggagactta
     9481 ttatgtagaa ttggtggagg aagtagtaat catgttgtaa tgtacattgg aaacaatcaa
     9541 ataattcatt caccacaaac aggagatgtg gtaaaaatag agtctgttaa ttcttataga
     9601 aaaggaaaag catacacaca tgtgagaaga tttatataag tgaggtggca atatgagcca
     9661 agatttatta cagataataa aaaaagctgc aatggatgca gtagaaacaa gcaacccaat
     9721 gagggttgta tttggaacaa tagaaagtat tagtcctcta agagttaaga tagaacaaaa
     9781 actatctatt ggtgaaattt ttctaataca aacagataca tttaaaagat atacagataa
     9841 aaaaatggga gataaattag tcttaattcg tatgcaagga gggcaacaat acttgatttt
     9901 agataggatg tgataaagtg ttaccaagtg ataatttaga ttatgatatt gaagatgtat
     9961 caataattaa ttttgatgta agacaagagc caagtaagac atttaaactt catatagaaa
    10021 agtctaagat tgatggtatt tgtgatgatg tagaagcatt aaaacaaacc atttttttaa
    10081 ttttaaatac tgaaaggtat gagcatctta tttattctag aaattatggt gttgaattaa
    10141 atgatttaat tggagaacct atttcatatg taatacccga acttgaaaga aggataacag
    10201 aagcactaat tcaagatgat aggattgaaa atatagataa ttttgagttt caaaatataa
    10261 agggtaaagt acaatgtaga ttttcagttc atacaaaata tggaaatata aaagcagaga
    10321 aggtggtgag tgtataattg tttgagttaa tgacatttga aaatataatt aaaagaatgt
    10381 tagatagtgt accagatact tttgataaaa gggaaggttc tataatatat aatgctcttg
    10441 ctcctgttgc tatagaactt acagaaacat acattgctat ggatgaatta ctagaccaaa
    10501 cattcgtaga tactgctagt tattattatt tagagaagag atgtaaagaa aggggaataa
    10561 caccacttga agccactcat acgattgcta aaggagtttt taacatagat attccacttg
    10621 attctaggtt taatctagga gaatataact atgtagcaat tgagagaata tctgaaaaaa
    10681 catataaaat gaaatgtgaa actactggac ctgtatttga gttaggaaaa ctaataccta
    10741 ttgaatatat agatggtctt gaaactgctg aactaactga aatcttgata aatggagagg
    10801 atgaagagtc agaagatagt ttaagacaaa gatattatga tagcctaaat tcacagagtt
    10861 ttggcgggaa tatacaaaac tatagggatg aagttaacaa aatacaagat gttggaggag
    10921 ttaaagttta tccaacttgg gatggaggtg ggactgttaa gttagtaata attaactcta
    10981 attttaaagt gccaagtgaa gaattagtaa acttagttca agaagaaatt gacccaattg
    11041 gacaccaagg agaaggctta ggattagcac cgattggaca ccgagtcaca gttgaaggag
    11101 ttacaagtac aactataaac atatcagcag aaataacata caaaaatggc tacacttggg
    11161 agaatataaa atcaattgca gaagaagcaa tagacgacta tttaaatgaa cttaacatga
    11221 gttgggaaga tgaagaaaac ttaatagtcc gtatatctca aattgaaact agattactta
    11281 gtattgatgg agtattagac attgcaaata caatgataaa tgatgttaaa tctaatctaa
    11341 caatagatag taacagcata gtagtgagag gtgaggtagt tggataaaga gattaatcta
    11401 ataaattact tgccacaaat tctacaagat aaagaagaat atataaaagt atttaatgta
    11461 gaaaataaag aaataaaaac actacatgat aaattaaagg acctatcaaa tgaccagttt
    11521 ttagaggacc taactataag tggtataaaa agatgggaaa agataatgtc tataactcct
    11581 aaaagtaatg agagtttaga agatagaagg tttaggattt ttagtaaata tataagtaaa
    11641 ttaccttact cagagagatt tttaaggaac tggctagata atgtagttgg agaaggcaat
    11701 tatgaattaa ctattaataa tgctacttat aacatacacc ttgagagtga tgctagaaat
    11761 caagattggt ttgaggaagt tcattctttt gtaagtaata ttaagccatg taatatgact
    11821 ttagattaca ctagagtgct tataagcaaa gacaattata tgaattttgg tataacaacc
    11881 ctaatgggtc aagaaataac tatataccct tggagtccac cagatataga aacttatgga
    11941 gaaattgatg tattaactgg caatggagtt ggataccaag agataacaat attttaggag
    12001 gtgatatatt ggctatagat aaaagttatt acactataat tacagatgta ggaaaagcaa
    12061 agatagcaaa tgcaagtgtc acaggtaata aagtagggtt tgtaaaaatt caacttggtg
    12121 atggtggagg aagtgagtat actcctactg aaagtcagac agaacttaaa aatgtggtat
    12181 gggaaggcaa catcggaaat acaaccacag atgaaactgc gccaaactgc ataatattag
    12241 agagtttaat accatcaagt gtaggtggat ttatgataag agaaatagga tatttagatg
    12301 atgaaaataa tctaattgca atctctaaat ataaagaatg ttataaacct tctatagaac
    12361 aaggagcagt tgttgatatg aaggttaaaa ctgtgcttat tgtatctaat ataaataata
    12421 tagaacttaa aattgaccct acaataattt ttgctacgct caaagatata caggatttag
    12481 atgctaaaat aagcaatgta aacactaaaa ttgatacaac taaaacagag ttaacaagca
    12541 acatagaaac tgctaagaca gagttaaaca ctagaattga cacagaaaat gagaaacaaa
    12601 atattaaaat tgaccagcta atagcaggtg gttcaaatgt tgcatctact caaacaataa
    12661 caattgatga ttggatagat aatcaagaag gtggattcaa agcaactgta acacatggtt
    12721 tattaacaca gagaataact gtaagtatta tagatgctac tacaaaagat aatgtagtac
    12781 ccgatttcac aattatagac gataattcta ttgaggttag aagtggagta aaagttgagt
    12841 taaatgttta tgtgattaat gggaatgcag agactcattt tattaatgcg actgtagatg
    12901 ataatagagt gtctgaaatg actacttatt catcaaaaaa gatacatgaa gaaattggta
    12961 aggtagcaga gcaactgaca ggaattaata gtaatattat atcgactgta aataacgaca
    13021 tactacctat atagaaagga gcgtgaaaaa atggcgttat caatgagtta ttttaattta
    13081 cctgacaaaa gaaaatatac taaaaatctt gcatttaatc catttgcagg tggaaggcaa
    13141 aattttgagt ggactggtgg tgaccatgga ctaaatggtg agtttaaaga aacttgctta
    13201 agttgcactt acaatggttc aacattaaat tggggttcag gaaatgtttg ggtcttgggg
    13261 gaatatggtc aatatacatt tacttataat tgtgaatcaa tgcatgtgga tacacaacaa
    13321 aaatttccgt atacttctaa tagaataata actataaaag gtagacctgt aatatctggt
    13381 tcagatactt cacttgggaa taaaagaaaa ggatttagtg tagattttac tgtaagtgat
    13441 gatactccaa atgttaattt aatagtacgt gcttatcttg atgataaatt aatacaaaac
    13501 attactcctg ttgtacaaaa ttcaacttta acagcaacag taacagatag tcaactaaac
    13561 tctttatccg tagatggaaa tcacaaacta aaaatacaat taaatgatgg atatgataat
    13621 tttgatagaa tttttacttt taaaaaaata gaaaagggaa ttgatatatc aacatcatta
    13681 gtaactgata gccaagctaa gtttactgta acaaaaatat acagtgaatt gacaaaaata
    13741 gagtgttatt tagatgaaac attaaaagag acttttacta cagacttgta ttcagaaaag
    13801 actataaact atgaacttat agacaatgca atacacacat taaaaatagt tgtaactgat
    13861 gcagaaaatg ttgtagaaga aaaagttata agtataagta agaatataat gccattgcaa
    13921 cctgatgcaa cattacaaga tatatcaact aagttaacag agattgggca aggcgttaga
    13981 aatggtaaaa caagcattat aaatacttta gcattaaaga atatagatgc aagtttaaat
    14041 aatacactcg ttgagttatc agagaaaatt aagggaggtt ttgatagtgg agacgctagt
    14101 ttacaagatt tgatgaatca attaacacaa gctaataata ctatatcgca gttaaatact
    14161 aagtataaag ttgctagtgg tacagttact tcttttgcgg atagtactaa aattgcttat
    14221 ccatatctaa ccgacaatgt tactaaacct ggttcttgga ttaaagttag taatttaggc
    14281 tttaaaccta atattttctt tgctgatttt gattactatg atgctgaata taaaaataat
    14341 tataaacttt tccttttcgc ttgtaatggt gttgccacac aaagaggtgt tgatttttca
    14401 agtgttacat cttttatcag aaaaagtggt gatgagtatt ttcatgctaa tggatggcta
    14461 tatagtaact cagaggggga tgtctatttt aataatactg gcgttcagat tccagcttat
    14521 aactttgatt caactcaaaa acatacttat aaatggtatg ctattaaatt tatttaaaag
    14581 gaggaaaaaa catgaatgtt ccaaaccgaa taatatatga ccaaacaggt agaacaatct
    14641 ttgaaacagg cgaatcctgt ggagatgtat tgccacatta tacaataacg gaattgcatt
    14701 atatagatat tgaatatgga agtatagatt atacaaggaa tagagttata ggtataaata
    14761 tagaaacaaa agaaccgatt ttagaagaaa taccagtata tataacagat gaggaaaaga
    14821 gaatacaaga gttagaaaat caattattaa ttgcagaaaa tgagaaagta ggaggattat
    14881 tataatgaat ataaataatg ttgtagtaag aatattagca gaaagaatat taagtaaggg
    14941 attaaatcct ctaaaaaata gagaatttca gttagatgac gtgactaaca cagagtacag
    15001 aaaagctgta gaggattata ttataaaaaa tagtggagta gtagaaggag cagaaccaac
    15061 tatatagagg gttctttttt tattgaaaga aggtgactaa atgactttta aagagttggt
    15121 taataaagtt agaaatcttg tattagaagc aaagaatgta actatagaag atacagaaag
    15181 taaatttaca agtgaaaatg tagaaggagc attgaaagaa tgtatagata gagcagatga
    15241 ggcttttcaa gaagccgata gtggaaaaac acttttatca actgctatcg gctctcctac
    15301 tacatcagaa caaacatttc aagattatgc gaactatatt acaggattta agagcaatat
    15361 aagcaattta gaaactcaat tgaaaagcaa atattctatt agatatggtc ctattgatgg
    15421 atatgatggt aatccttttt ctgctaattt tggcaagagt gcaagttacc ttattgtcta
    15481 tgtttacttt agaagaagtg tatattatta taatcccagt ggtagttctt taggaagtaa
    15541 tacaggaggt tctgaacgtg catggattac tataaacagc aataaaactg gtttttcagt
    15601 tcattcatat gatactagtt atgagtcata tccttttaca ggttattata ttgcttgttt
    15661 cgcataataa attatattat taaaactagg aggatgtatg gaagaaatta gcataaatct
    15721 actatgtgca gtagcaggag ttgtaatatc ctacttagca tttagaaata actcaaacag
    15781 aaagatacaa gatgaaacag aaacaactac aaaattagaa caacaaataa cttttttatg
    15841 tgagaatgta agagatataa agcatgatgt agcaaagttt aatactagtt tcttagatat
    15901 tagtgaacga gttgcaaaaa tagaagcaag tacaaaacaa gcacatctta gaattgatga
    15961 aattgtaaat agaattggag gaaaataaga gatggataat ttaataagtt ttataccaga
    16021 gcagttgcta attttagtag ctgctctctc tattataggt aaaggttgta aaaaatataa
    16081 acaattagat aataaataca ttccagtagt gttactgata cttggaatag gtttctcaat
    16141 atggatgcta ggattaaatc ctgttgcagt cttacaaggt gtaatttgtt ggggagttgc
    16201 aataggtata aatcaaactt acaaacagtt gaaggatggt gaaaagtaat gaaattaaca
    16261 aaaatattat tactcacaaa aatttaaaaa atttcattaa atagagtata cttttatagc
    16321 taataagtat ataataatag tacagaactc gtaaagcatt tatatgctca aatatacggg
    16381 acattgattt ttgaaggaac ccgccaagct tcttttatag ctcaaatagg cgggacagat
    16441 aaatttaccc actaatatta attggtgggt tttattatat aaaagtatat ctatgaaaag
    16501 gtgaaaacaa tggttgaagt aaaagaagaa aaaacatttg atgaacaaat agatatttta
    16561 aaaagtagag gattaataat aaatgataaa gaagatgcta aatttgtatt aagtaatgtc
    16621 aattattata ggtttacagc atatcttcta agttttaaga atgatgatgg ctcatataaa
    16681 gaaggaacta cgtttgaaga agtttatgat atatataggt ttaataagga atttaggata
    16741 ttattaacag atttgttagg gagcatagaa atagcattta ggacatacat tgcatataca
    16801 ttagcaatta aacatggtgc ttgtggatat ctagaaaggg agagtttcaa agatgaaaaa
    16861 ttctatatta attttttgac agcattagag agagaaaaaa gtaataattc agataagctt
    16921 tttattatac accataaaga aaaatatgaa ggaaaacttc ccatttgggt tgcaacggaa
    16981 ataatgactt ttggtatgtt gtcaaaatta tattcaaaca tgttgccaga agatactaga
    17041 tacataaaaa ataatttgtg tagagtgaat actttattag ttaaatcttg gttacaatca
    17101 ttaacacagg tcagaaatca atgcgctcat tatggtagaa tatataataa taatttccgt
    17161 attataacaa taaaaaacga atataaaaag tataacttgg ataataaaaa gatattttct
    17221 tatatacttg ctatgaagca tttgactatg gataaattaa tttggaatag tttttttata
    17281 aaacttcaaa agttaattaa tgattataat aattctatag acttaaagct tattggtttt
    17341 cctaataatt ggatagagat attggctaaa taaaatagtt actttaagaa gtttataaac
    17401 acttactata tgtaagtgtt tttttattga aaagaaggag gaaaataaat aatgaaaata
    17461 tgtattacag taggacacag tattttaaaa agtggtgcat gcacttctgc tgatggagta
    17521 gttaacgaat accaatacaa taaatctctt gcaccagtat tagcagatac atttagaaaa
    17581 gagggtcata aggcagatgt aataatatgt cctgaaaagc agtttaaaac taaagcagaa
    17641 gaaaagactt ataaaatacc tagagttaat agtggaggat atgatttact tatagaacta
    17701 catctaaatg caagtgatgg tcaaggaaaa ggttcagaag ttctatatta tagtaataaa
    17761 ggtttagagt atgcaactag aatatgtaat aagctaggta cagtatttag aaatagaaga
    17821 gctaaattag ataaaggatt atatatctta aatagttcaa atcctacagc agtattaatt
    17881 gaaagtttct tctgtgataa taaagaagat tatgagaaag ctaagaaact aggtcatgaa
    17941 ggtattgcta agttaattgt agaaggtgta ttaaataaaa atataaataa tgagggagtt
    18001 aaacagatgt acaaacatac aattgtttat gatggagaag ttgacaaaat ctctgcaact
    18061 gtagttggtt ggggttataa tgatgggaaa atactgatat gtgatataaa agattacgtg
    18121 ccaggtcaga cgcaaaatct ttatgtcatt ggtggtgcag catgtgagaa gattggttct
    18181 atgactaaag aaaaatttac tatgataaag ggtaatgata gatttgatac actttataag
    18241 gcattagatt ttattaatag atagattata taaaatattt tagtatttct tgaaaatcat
    18301 aagtatggta atatttagat aaatattatt tattattaat aggtatgggg ggagattatg
    18361 atatttaaga agtatataaa atcattactg atagttgtta gtgttacatt gataatgaac
    18421 aaatcaatat tatttgtaaa tgcattagat ttagagagta acaataacat aaaatcttat
    18481 aaaagaaaga atgtaaagga aattgatata agcaataaat ttgttgatga atcctttaga
    18541 agagatattg ttgaaaaggt attagataat agggatgatc cattaacaaa tgaagttgca
    18601 tcaaaagata actataaaat atatgaaagt gatttaaata aaattgagag ttgcttagag
    18661 ttagatttac ataaaaatcc agatgtttct aatccaaaca aaatagataa tttagatggg
    18721 atagaaaatt ttagaaatct agaaaagctt gattgtagtt caaataatct agaaagtgta
    18781 gatttaagta aaaatataaa attaaaatat ctagatatta gtggaaataa aatattaaac
    18841 attgatttaa aaaacaataa tcttattaat gaattacatt gtgatgcaaa taaattatca
    18901 aatttagacc ttaaaaataa taaaaatttg gtaagtttat tttttactag taatgaatta
    18961 acagagatag atttaagtaa taatgtgaac ttagaaaatt tacaatttct taataataat
    19021 ttaaaaagta tagatttaag tcataataaa aagttgaaat ctctaaattg tactaacaat
    19081 aaacttaaat cccttgattt aagtaataat gtttttttga aaaatttaag ttgttcaggt
    19141 aataacctga gtaaattaaa tataaaagga cttacaagtc ttgagtattt gggatgtgct
    19201 aagaataaat tacaagattt agatgtatct acaaatataa aattagattc tttactttgt
    19261 gggaaaaata gtttaacaaa aatagatata agtaaaaata aagaattaaa ttcactggaa
    19321 tgtagtggtc aatatataga aaaaagcatt ataaaaaatg gtgaaaatat agttgtagat
    19381 ataggaattg aaaattcgaa attaaaggaa ttatatccac aaaattataa tatttcaaca
    19441 ggttttctaa catgggaaaa tctttctgac tttccaaata gcataagtta tagtttggaa
    19501 tatgaatatg gtccagtaaa tgatacagag actatggatg taaatatatc aatagataaa
    19561 ttagtgaaat gtagaatcag atatatagat tatgatggaa gtataattaa tgaaaaagaa
    19621 atatcagata attctagtat aaatgaaatt caaattaatc ctcaaagaga aaactatgag
    19681 tttagtggtt ggaggaacaa taaagatgaa aaaatatata catcagagca ggtaagtaaa
    19741 ttagtttttg atgatagtac tgattttata gcaacttata aaagaaaatc aacaggatca
    19801 agtggttctt ctggtggaac ttcaagtaaa gaagatttta atatagcacc atctaaggaa
    19861 aaaataacag gtgcagacag aaatgaaaca tcagtgaaaa taagtcaaaa aggatggaat
    19921 aaagctgata atatagtgtt aataaatgac tctagcatat cggatgcttt atcagcaaca
    19981 ccatttgcta agtctaaaga tgcaccaata ttacttacta aaaataataa tttaaataaa
    20041 ttaacagaaa aagaaataaa tagattagaa gcaaaaaatg tgtacatagt gggtggatta
    20101 aagtctgttg atgaaaaagt agtgtctgat ttaaagaaaa aaggattaaa tgtaattaga
    20161 atatctggaa atgatagata tgaaacatca ataaaacttg caaaagaatt agacaaaaat
    20221 tctaacctgt caaaagtagt agtggtaaat ggagaaaagg gactagctga tgcagtaagt
    20281 atgggagcta tatcagcaaa agaggaaatg ccaattcttc ttactaatca aaatgatgat
    20341 atgaaagata taaaagactt aatagctaat aaaaacatat caaaatcata tgtaataggt
    20401 ggagaatctt tatttaataa taaagaagtg aataatacat taccatctgt aactaaaata
    20461 gcaggttctg atagaacaga aactaactca aaagtaataa gtcatttcta tagcaaagat
    20521 acacttaatg acttatatgt agctaagaat ggaatgaata aacaagacga tttagtagat
    20581 gccttatcag taggagtgct tgcaggtaaa actgaatctc cagtagtatt agttggaaat
    20641 ggattagatg atagccaaaa agagttaatc aagaataaaa agtttaaaaa tataactcaa
    20701 attggtggaa atggaaatga aaaagcattt aatgaagtag aaaacttagt taaatagatt
    20761 gaaaggtagt aactggaaat agttactacc tcctttttta ttcattttca gttttttcaa
    20821 taattatttt tccatcctca aaagtaacaa gaacatccct ttcatcagga gagatattca
    20881 tctctttaat ccatgacatt ggaagtatca atctgggtgt aaaagagcct ttgccacttt
    20941 tattaaatcc aattctaagt tttctttgct ccaataatat cattcctttc tactattaat
    21001 aaatgccagc aataataaag taaataccaa agcaataaga gctttaaaag taaaacttat
    21061 tattatatta ataatattta aaactatagt gataccaaga ataatcatac aaattctata
    21121 aaatttatta ttcatattaa attagaaaat gtgttataat attagaaaga agctacctag
    21181 aaggaaggta ttttcctcct aggattgaaa cttataattc tttaatcaat cttagtattg
    21241 cgagtactag attaattaaa actaatgtga aagagaaagc atttttaatt ttattaaatt
    21301 tgttttctct tttatttttt cttctttcta atctccccac tttcttacct cctttctata
    21361 tttatattat atcatcacgt gacgtgaaag tcaataaata ttacatattt ttcaataaag
    21421 ttatattgat aaactatatt ttttacaaat aaaaataaat ggtgacaaaa ccaaacttgc
    21481 caccattttg ccaccgaaaa taaataatag aataaattta aattaaacat aataaaacat
    21541 aaacaacaaa actgacctta ataaaacatt gaaataccaa cacttgtaaa aaataacaac
    21601 aataaacaaa taacctagaa taatattaca acttaagtaa ataataattt tgaataataa
    21661 tttacggagg tattcatatg tttaaacacg ataaagcatt attaaaagaa gtaaaagtag
    21721 aaagaccaaa tcctcaatat gctgttttaa tgcaagaaca gttgggtggt gcaaatggag
    21781 agttaaaagc agctatgcaa tacttgtccc aaagttttag aataaaagac cctcaaataa
    21841 aagatttatt tcttgatata gcagcagaag agcttagcca tatggaaatg gtagcacaaa
    21901 ctataaattt attaaatgga catgatgttg attataatgc agttaataca ggcgaaatag
    21961 aaacacatgt attaactggt ctatcaccag tcttaataaa ctcatcagga gctccttgga
    22021 ctgctaatta tgttacagtt acaggggatt tagtagctga cttactttca aatatagctt
    22081 ctgaacaaag agctaaggta gtttatgagt acttgtatag acaaatagat gataaatatg
    22141 taaaagaaac aatagatttc ttacttaaca gagaagaagc acataatgct ctatttagag
    22201 atgctttaaa taaagttaaa gatacaggtt caaatagaga ttttggagtt acagaagatt
    22261 ctagattata tttcgatttg tctactccag ggccaaatca caatactaag atagatatta
    22321 atcctccttc ttttgaaaaa cctttaaaaa aataatttga tattttttat gttgttttgt
    22381 tttagtagtt ataaaaatta tttactagaa gaattaatta tatgaaaaat taaatatgta
    22441 tagttaaaaa tgggtataca cttattataa gtgtatatct atttgtttat tgcaaaacat
    22501 ataataaaat aacaaatgag gtggtatttt ggacagtaat agaaaaccta ataatctaat
    22561 aaatgaaaaa tctccatatc ttttacagca tgcttataat cctataaact ggtatagttg
    22621 gaatgatgag gcttttaaaa aagcaaaaga agaggataaa ccaatatttt taagtgtagg
    22681 atattcaaca tgtcattggt gtcatgttat ggaaaaagaa tcttttgaag atgaagaagt
    22741 tgctgaaata atgaatagaa actttgtggc aataaaagta gataaagagg aaagacctga
    22801 tgtagatagc gtgtatatga cagtttgcca agctatgaca ggtagtggtg gatggccaat
    22861 gaccataata atgacaccag ataaaaaacc tttttttgcg ggaacatatt tcccaaaata
    22921 ttcaaggtat aatagacctg gagttataga tttattaaag aatgtatctg aaaaatggaa
    22981 tacaagtagg gatatattaa taaaatctgg agatgaaata atagaagctc ttaaggatga
    23041 ttttggtgta aaaaatactg agggtgattt atcaaaagag atgttgagtt catcagttag
    23101 agtatttaag gccatttatg atgaaaagta tggtggtttt ggtaatgctc ctaaatttcc
    23161 aagtcctcag aatcttatgt ttttaatgaa atattatagc atagaaaaag ataaagatgt
    23221 gttaaaaatg gtggaaaaaa ctttagatgg aatgtataga ggaggcttgt ttgatcatat
    23281 tggatttggg ttttcaagat actctactga taaaaagtgg ttggctcctc attttgaaaa
    23341 aatgctatat gataatgcta tgcttactat agctttttta gatgcatata aaataacaaa
    23401 gaaagagtta tataaagaaa ttgctataaa aactatagat tatgtagtta gagagatgaa
    23461 ggataaagaa ggtgggtttt attcagcaca ggatgctgat agtgaaggtg aagaaggtaa
    23521 attttataca tttaatcctc ttgagataat tgaagttttg ggtgaagaag atggaacttt
    23581 ctttaataat tattttgata taactagtag tggaaatttt gaaggtaaaa gtattcctaa
    23641 tcttataaaa aataaagaat atgaaagaca taatgaaaag atagctgacc ttagtaaaaa
    23701 ggtatttgaa tatagaaaag aaagaacatc tttgcataaa gatgacaaga tacttacatc
    23761 atggaatgca ttgatgatag ttgctttaac aaaggcatat agtactttaa aaaatgacat
    23821 atatttagag tattcaaata agtgtcttaa ctttataaat aataatcttg taaatgagtc
    23881 aggtagatta ttagctagat atagagatgg aagttccgac tatttagcat atttagatga
    23941 ttatgctttt ttaatctggg cgtatataga gctttatgag tcaacattta atatgaaata
    24001 tttagaaaag gctttaaatt tgaatgaaag ctgtatcaac cttttttggg attatgaaaa
    24061 aagtggattt tatatatatg gtaaagatag tgaaaattta atagcaagac caaaagattt
    24121 gtatgatgga gctattccat caggaaattc tgttcaatta tacaatctta taagacttgc
    24181 gaaaattact ggagataata gacttgaaga aatgtcatat aaacaattaa aactgtatgt
    24241 ggataatgta aagagttctc caactggcta cagcttttat atgctttcat tgatgtttga
    24301 actttattct actaaggaaa ttatatgtat ttttaaggaa gattctgatt taattgcatt
    24361 taaagaattg ataagtgaaa attttatacc aaatgcaact tttttagcaa agaaatacaa
    24421 tgaagaaaac actattatag gttttttaaa taactataga ttgaaggatg ataagactag
    24481 ttattacgtg tgtcaaagta atagttgtag tcaaccaatt aatgatttac aaaaattaaa
    24541 agatatgatt ttaggagtag aataatcata atagcgaaaa aggagatgct ttaaaataaa
    24601 agtctaatca tctccttttt ttgtacaaaa aacagaaaaa gagttcctaa gaacacctaa
    24661 tctatgtaat gattgttttg ttaaaaaata aaaatgacat tatagttata taaaatattg
    24721 tactataaaa aagtggtatt atagatgcga tagtattaat cgtttttatt taccctattt
    24781 cttctttttg caaattcttc aagtgcttca gatttaattt tatctatttc ttcatattcc
    24841 ataaaattag catcttcttc aaagccatct tgttcttcac cattaaaacg atacattttt
    24901 tttagtaaac tccataaggt ctctaattca tctttggtaa attcgtgaaa tacatctgcc
    24961 ataaagttta ttccagtcct actacatgtt accataacct tttttcctaa gtctgtaact
    25021 ttaacattaa ttgctctctt atcatgagga cttggaatta catcaacata tccatttttt
    25081 tcaagattag caactagtct atttatattt tgtttacttg tacccatttt tcttgcaata
    25141 ttatttaatg ttgtttcttc ctctggtaaa tgaagaattg ataaaatagt catatactgt
    25201 cttgaagtaa gtataccaaa atatttatct ccttctattt gtattttgtt tgtaagggta
    25261 aaaagagttg catatgtttg attcattaaa aagagctctt ttagttcatt tgaataatcc
    25321 atgaaatata tctcctttat ttttagaagt tgttttgtcg aaaacagaat atcatgaatt
    25381 aattctaatg tcaatatggt gtcaaaatat attaaagtat tactttttta tgtaactatt
    25441 aattaaatta ttgtataatt gtatgtaaaa acttctatgt accaatgata atttctatga
    25501 ggaattaata attcttaaat tttttagaaa gattttgact tgaaaatact tgtaatataa
    25561 tatataaatt aatatactga ataaaatcaa tctggaggga taattatgaa gtgtacaaac
    25621 tttactttca aaggagagga aggtttagat atatatacat ataaatggga agatgaaaat
    25681 ataaaaaaac caaaggcagt tatccaaatt gcacatggaa tggctgaaac tgcacaaagg
    25741 tatgagactt ttgccaaggt acttactaaa aatggttata tagtatatat caatgaccat
    25801 agaggtcatg gaaaaactgc aaagataata gaaaatgttg gacatttagc tgaaaaagaa
    25861 ggatttagat gtcttgtaga ggacatgtat actttaacaa atattataaa gaaagaaaat
    25921 gaagatttgc caatttatct atttggacac agcatgggtt catttgcaag tcaaagatat
    25981 ataatggatt acagtaataa tttatctggt cttatactat gtggttcaaa tggaaagcaa
    26041 ggaatcattc taaatcttgc tcatctgata atcaatcgtg agattaaaaa gtatggaaga
    26101 cgttctaaaa gtaataaaat aaataatttg attaaaaaat agcta
//
LOCUS       DAMJVX010000035        26121 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMJVX010000035 DAMJVX010000000
VERSION     DAMJVX010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26121
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.037"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            157..1605
                     /locus_tag="PC407_001401"
     CDS             157..1605
                     /locus_tag="PC407_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2496855.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIIHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            1631..4021
                     /locus_tag="PC407_001402"
     CDS             1631..4021
                     /locus_tag="PC407_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF2496856.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGIVKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIELKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKDWI
                     NAAQNIYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLNYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKKDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGKNISILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            4023..4694
                     /gene="pgmB"
                     /locus_tag="PC407_001403"
     CDS             4023..4694
                     /gene="pgmB"
                     /locus_tag="PC407_001403"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF2496857.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLERGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            4733..6133
                     /locus_tag="PC407_001404"
     CDS             4733..6133
                     /locus_tag="PC407_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2496858.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNENKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIEKFINKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVKESTDNVVEEV"
     gene            6450..7292
                     /locus_tag="PC407_001405"
     CDS             6450..7292
                     /locus_tag="PC407_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2496859.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            7437..9365
                     /locus_tag="PC407_001406"
     CDS             7437..9365
                     /locus_tag="PC407_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2496860.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKFVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMTIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVASPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            9426..10853
                     /gene="ascB"
                     /locus_tag="PC407_001407"
     CDS             9426..10853
                     /gene="ascB"
                     /locus_tag="PC407_001407"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2496861.1"
                     /translation="MSFKDDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYRGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLVGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     INYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            11264..12040
                     /locus_tag="PC407_001408"
     CDS             11264..12040
                     /locus_tag="PC407_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2496862.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IVKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            12056..12421
                     /locus_tag="PC407_001409"
     CDS             12056..12421
                     /locus_tag="PC407_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2496863.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(12587..13336)
                     /locus_tag="PC407_001410"
     CDS             complement(12587..13336)
                     /locus_tag="PC407_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2496864.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     GKPFIFVCPTYAWRLPIVVTDFIKETKFLGDNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNS
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(13781..14338)
                     /locus_tag="PC407_001411"
     CDS             complement(13781..14338)
                     /locus_tag="PC407_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2496865.1"
                     /translation="MRTLKYAILGLLNRNPMTGYDIVKEFNFQLAEFWNAKHSQIYPE
                     LKKLVNEKLIVYDIKISGDVLEKKLYTITEKGKDEFLKWLEKDEPMERTPKDIFRLRM
                     YFSNNLDIDTRLILLEHQLLQHQERITFLYSQMERYSEVPPLDSNDFGDYIVLDGAII
                     REQGAIKWLTNCINHHKNLDNTTLK"
     gene            14590..15888
                     /locus_tag="PC407_001412"
     CDS             14590..15888
                     /locus_tag="PC407_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HDF2496866.1"
                     /translation="MNLTQKRWIILIASCFINLCIGSIYAWSVFASPMAEHLNSLAGT
                     TFTAASLAIVFTVTNSVGPITMITGGMINDKFGPKKVVFVGGLIFGIGMILAGFSKGL
                     PMLILSYGIITGLGVGMVYGCTISNSIKFFPDKSGLVGGITTAAYGFSSVIMPPVANW
                     IINKYGVTSAFKSIGLVFLIIVCVSSFFIKKCPADFTPEGWVPPEKKGKVRVVINKNW
                     KEMLSTPIFYLMIIILTCGAFYGLMCVSLASSLATNMIGMTSSDSAIVVSILALFNTL
                     GRIVAGYISDKIGRINTLSGALVISIFGLLCLLFSREGDVATFYIGIIIIGICFGSFM
                     GIFPGFTVDKFGPKNNSVNYGIMFIGFAFAGYIGPAVMKNVYSVDGNYQRAFIIAIII
                     ALIGFILTFLYKYINKIQNLKIENLIKGDIKNGKKISKSM"
     gene            15860..17809
                     /locus_tag="PC407_001413"
     CDS             15860..17809
                     /locus_tag="PC407_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2496867.1"
                     /translation="MERKYPNLCKPIKIGNVTFKNRMFSAPMGGTDITADCTIGSKST
                     AFYELRAKGGAGAVTISECMVHPETDGSHAYHLDLKIVDSLASFTYTADAIRRHGAIP
                     SVELSHSGQYSGTYLVDKDKKRGLSQWGVSPSVRPDGLEIKELTEEIIAEIVESYGNV
                     AALAKRAGFEMIMIHGGHGWLINQFLSPYFNKRTDKYGGSLENRVRFAKEVLDSVRKA
                     VGPGFPIEFRMSGSELFEGGYDLDYGTEIAKLLESRVDLLHVSAGTYQRGFAVTHPSM
                     FLEHGCNVYLAEEIKKHVNIPVATIGALNDPEMMEEIIASGKADVIYMARALLADHEL
                     PRKVMSNQDEKIVKCLRCFTCMAERATTSTRRCTVNPLIGRELDGTEVVSVLKPKKVL
                     IAGGGPGGLQAAITAVKRGHKVILCEKTNELGGILKGEQALPFKYKMYELGNTFGKIA
                     KDLGVEVRLNTTVTKEYVENENVDALIIAVGSEPLVPPIEGLDGENVVIVNDYYVEKE
                     KVTDEVIVLGGGLAGCEAAIHLAQEEKTVHLVEMRAELAPDANIRHRPILLEEIERQG
                     IYVYTEHKGLSVTTEGVVCMNKSGNKINIPGTSVICALGQKPRREVVDTLLDSAPYVA
                     QIGDCVRASTITNAVYQGHHAALDI"
     gene            18246..18383
                     /locus_tag="PC407_001414"
     CDS             18246..18383
                     /locus_tag="PC407_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2496868.1"
                     /translation="MINENLKSLRKIHQYTQEELAEKLNVSRQSIAKWESGVSQTKGY
                     Y"
     gene            18611..18985
                     /locus_tag="PC407_001415"
     CDS             18611..18985
                     /locus_tag="PC407_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDF2496869.1"
                     /translation="MFNLIEMLFPIMFILIFIIIVFTFVKGIATWFKNNNSPRLTVSA
                     RIVAKRQNTTHNNQPNAGDISGAHGFHTISSTSYYVTFQVDSGDRIELSVSGSEYGVL
                     AEGDKGQLTFQGTRYLTFDREN"
     gene            19001..19648
                     /locus_tag="PC407_001416"
     CDS             19001..19648
                     /locus_tag="PC407_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496870.1"
                     /translation="MNKFLSQIRRIDNEISITRKIINTIFILCFGIVLGTFAKFLDTT
                     ASNTLPFIFEYLDISNFFGRFAIWLLIALYIAIYSHSSIRASLNVLVFFIGMVSSYYL
                     YSYFVAGFFPKNYAMIWLGFTVISPLLAFICWYAKGKSKISFILSVIIIAILFNFTFI
                     YGWIYFDVYSILEVIVFGCALIALKRNTFRETTYMILSAVVIAVILNMLVPFHFG"
     gene            20028..21107
                     /locus_tag="PC407_001417"
     CDS             20028..21107
                     /locus_tag="PC407_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2496871.1"
                     /translation="MNDMKFIETLKQKRNVCDYSQSRLAQELQINRQNLNEIENGKTK
                     ASKEMKHILLHYLDYCNCTQPFTLTIDYLRVRFPTTDALEIIKNVLAMKSEYFIHEDY
                     GMYAYEEQYIYGDISVNASKDSSMGVLLELRGMGCRDLEYVLQVRGIDWYCFLRCCID
                     YQGVFKRIDLAVNDMGGLLDIEILRERYYANKVWKRSRTHEAVDSGKLSGTHGDTAKT
                     FYIGSKNSSIYFCLYEKEKEQKSKGIKTDIKNRFEIRLKNGKAGQTVEQLVFSRNPEQ
                     TIASFILTQIDFPDYILWDIFLDNVTTSLPFIMTPVAVNMDKIKRWLERQVMPSLLMI
                     KEIEKKTGANYLEEIDRHTRLTEKQ"
     gene            21929..22142
                     /locus_tag="PC407_001418"
                     /pseudo
     CDS             21929..22142
                     /locus_tag="PC407_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
     gene            22254..23867
                     /locus_tag="PC407_001419"
     CDS             22254..23867
                     /locus_tag="PC407_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2496872.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFERPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIVEKIFSLAYQGYGSAKITKVLREEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     ATNTANKTPYSYYACSYYGQFGKGTCSMHYIRYDVLYQAVLKRLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASAFKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITNLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEAITNEDNERIQEIEIYYRFIGKVD"
     gene            23896..24219
                     /locus_tag="PC407_001420"
                     /pseudo
     CDS             23896..24219
                     /locus_tag="PC407_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
     gene            24251..25438
                     /locus_tag="PC407_001421"
     CDS             24251..25438
                     /locus_tag="PC407_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDF2496873.1"
                     /translation="MSKIVFFSILAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIK
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFITHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            25630..26034
                     /locus_tag="PC407_001422"
     CDS             25630..26034
                     /locus_tag="PC407_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDF2496874.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYHALYVNKVMLQI
                     YDVENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT         9888 a         3088 c         4826 g         8319 t
ORIGIN      
        1 tgaaagtatt gaaaatagac aatattaatt atagtgagta aaaaatataa ttggattttt
       61 aaattgaatt atttaaaagg tatagtatga aaaaaatctt ataactaaat atgagataga
      121 aattataaaa ctttatactg tgaaagaggt agatgcatga aattagggaa agatttttta
      181 tggggagggg caatttcagc caatcaaagt gaaggatttt atttagatgg aggaaaaggt
      241 ttatctcagt ttgataaact cccattaaat gataagagat taaaagatgt atgtttagat
      301 gaggataata ttttaaatga aaataatcaa tactatccaa gtcatatagg gattaaattt
      361 tgtgaaaatt acaaagaaga tatagcaatg ttagcagaaa taggttttaa atgttttaga
      421 ttttctatat cttggtcaag aatattccca acaggagaag aaagtgaacc aaatgaaaag
      481 ggattacaac tttatgataa tataataaaa gaattaaaaa aatataatat agagccatta
      541 ataacgataa gccactttga tataccttat tctctagttg aaaaatattc tgggtgggct
      601 gataggagag tggttaattt atatgtaaaa tatgctgaaa ttttgatgaa aagatataaa
      661 gatgatgtca aatactggat tccttttaac gaaatgaata tgataattca tattccatat
      721 ataggtgctg ggttaacttt tatagctgat gaaaatcaac ttcagaaaaa atatcaagca
      781 gcacaccatc aattattagc taatgcaaaa actatagaaa taggaaggta tataaataaa
      841 gaatttaagt ttgggtgtat gttagctgct ggaaaaacat atccatacac atgtaaacca
      901 gaagatgtat ttgcaacttt agagcatgaa aggcataatt tgtttttttc tgatgttcaa
      961 gttaggggtg tgtatccaag ctttatgaat tattatttaa aatcaaatca aataaaatta
     1021 ataataaaag aggaagattt atcaacactt aaggataata ctgtagattt tgtatctttt
     1081 agctattatt ctagtgcttg tagttcagct agagctgaag gtttagaaaa atcaaaggca
     1141 aacggaccag aaaccataaa aaatccatat cttccaaaat caaatagtgt atggcaaaac
     1201 gaccctgtag ggttaagaat aactatgaat cagttatatg aaaggtatca gttaccttta
     1261 tttatagtag aaaatggact gggtactcaa gatattgtaa atgaagatgg aaaaataatt
     1321 gatgattatc gtatagatta catgaaagaa catattaaaa atatgattct tggagtaaat
     1381 gaagatggaa tagagcttat aggatatctt atgtggggat gtatagatgt aaccagtgta
     1441 agtgaaggaa agatgacaaa acgatatggc ataattcatg ttgattctga tgactatgga
     1501 agaggctcat ttaaaagaac taaaaaacaa agtgcttttt ggtataaaaa agtaatagaa
     1561 acagatggag aaattttatt taatgatatg gaaactcatg cataattcat tttaagaaag
     1621 gagtgttatt atgagtttaa tatttactac taaggaccaa cttatagagc aagggtttag
     1681 taaggataca ataaaatcca atgaaacgtt gtttacacta gctaatggac atttaggagt
     1741 tagagggaat attgaagagt ccgattttaa taatgagtat gtagataata tgggtacata
     1801 tgtaaatgga ttttatgaag aatctccaat tacatatgga gaaagcgctt atggctatgc
     1861 aaaacaaaat cagactatct gtaagttacc taatgctcat atagtaaatt tctctattga
     1921 aggagattgc tttgatttat caaaaggtat tacaagtgaa catactagga tactagattt
     1981 aaatgaagga attgttaaac gttcatttat atgggaaaac tctcgtggta gaaaagttaa
     2041 agtatctata gaaagattag tatcacaaga tatagaagaa ttaatgatga tttcatatac
     2101 aataacacca ttgaatttta atggaaaaat aaaatttaaa aataaaatgg aaaatagtat
     2161 ggagaagttt aattgtgaaa aagtagatga cccaagagtg gcatctataa ataagaagca
     2221 atttaaaatt aatgtaaaaa aatataacaa taagtatttt atgaatttag ttacagaaag
     2281 aagtagatta gggttatggt gtgggtttga ttcatatatt acttgtggaa atattacaaa
     2341 tgagacctat aatgtttcag atgaatgtat tgagcgagaa atagaagtct atgccaaaaa
     2401 agatgaaagg ttaaatttag aaattgttgt aggctatagt agagtatatt ctgatttaaa
     2461 tctaaatgaa gaacgtttaa aatcattaga atgtctggag tcgttaaaat gtttagaatc
     2521 agaagcacat ttaaaatcat tagaattggt tttaagagaa gcatgtaata ttggatttga
     2581 taaagtcaaa aatattcaga gaaagaaaat gaatgagttt tggacaacct ctgatgtaga
     2641 gattgaaggc gataaaactc ttcaactggg tataaaaacc aatttatttc atatttatca
     2701 atcagcagga agagatggaa agactaatat atcagcaaaa ggtcttacag gtgatggcta
     2761 tgaggggcat tatttctggg atacagaaat gtatatatta ccatttttta tatatactca
     2821 accatccatt gctagagctt tattagatta tagatactca atcttagatg catcaagaga
     2881 aagagctagg gaacttggaa taaataaagg ttgcttatat tcatggagaa ctataaatgg
     2941 taatgaatgc agtgcatatt atccagcagg aacagcacag tttcatataa atgcagatat
     3001 agcttatggt attagaacgt attttgaagc aactaatgat gaagaattta tgatgcaaaa
     3061 tggcttagaa attcttatag aaacagcaag attttgggtt gaatttggag attatataga
     3121 attaaaaaat aataaattct gtataaatgg agtaacagga ccagatgaat atacagcaat
     3181 agtaaacaat aattactaca ctaatttaat ggctaaatat aatttaatat atgcagtaca
     3241 gatggtagat aaatttaagc ataatttatt ttcaaatgaa gtatttagaa aagttaaatt
     3301 acaagaatat gaagtaaaag actggataaa tgccgctcaa aatatttatt taccatatga
     3361 tgaagagaag aaaataagca aacaagacga ttcattttta gaaaaatcag tatgggattt
     3421 taaaaataca ccaaaagaaa aatatccatt acttctcaat tatcatccat taacaatata
     3481 tagatatcaa gtaaataaac aagcagatac tgtactttca gcgttgcttt tccctgatga
     3541 gtttagctta gaacaaaaga agagagattt tgaatactat gaaaaaataa ctactcatga
     3601 ttcatctttg tcaaggtcaa tatttggaat gatggcaagt gaaattggaa atcatgaaaa
     3661 agcttataac tattttatgg acacagcatt aatggatatt aataacctac aaaaaaatac
     3721 aagtgatgga atacatgctg ctaatatggg tggaacatgg atgagtttag tatatggatt
     3781 tgcaggaatg aaaataaaaa aggataaatt aagttttgaa ccaaggatac ctaaacactg
     3841 gaaaaatatt aaatttaata tattatttag agataattta gtttctgtaa atatagaaga
     3901 atataaatct tattataagt taatcaaagg taaaaacata agcatattac atgataataa
     3961 agaaataaaa ttatcaaaga acataataga agttaataat tgttttaaag ggagtaatta
     4021 atatgaaagc agtcttattt gatcttgatg gagttataac agatactgct aagtatcatt
     4081 ttgaagcttg gcatgagata gcaaaatcgc tagatataga aatagatgaa acttttaatg
     4141 aaaaattaaa aggcataagc agggaagaat cattagataa aatattgtta tctgctaata
     4201 aagtagatag ttatactaaa gaggaaaagt taaaggtgtg tgaagaaaaa aataacattt
     4261 atataaagtt aataaactca ttaacatata aagatatatt accagggata aatgagctta
     4321 taaaagaact taaaaaaaat aatgtgaaat tgggaatagc atctgctagt aagaatgcac
     4381 ctaggatatt agaatctttg ggaataaaag atgaatttga ttgtatagta aatccatcct
     4441 tattagaaag gggaaaacca tatcctgata tttttattga agggagcaag atgctaaatt
     4501 taaaacaata tgattgtgta ggaatagaag attcttattc aggtattaaa gctataaatg
     4561 ctgccaatat gacatcaatt ggtgtgggga aaaaagaagt actaggaaaa tgtgaccata
     4621 ttgtagaaga tactagctta ttaaattaca atacactatt acaagcttgg agtaaaaaga
     4681 ttaatttgga ttaaaattat attaaaaatt attttaaaag agaggataag gtatgaagaa
     4741 taaatatgaa gaagtagctc aattgataat tgatggactt ggaggaaagg ataatataat
     4801 tgacttaact cattgtatga cacgattaag gttcatctta aaagatgaaa agaaagtcaa
     4861 tgcagataaa ttaaaatcta tagataaggt agttggagta aactcaactt caactcaata
     4921 tcaggtcata ataggtaatg aggtaggtgt tgtatttaat gcaataataa ataaggggat
     4981 aaataccaca ggagatatta aaagcgatat aaaaaatgaa aataaaaaaa atagtatttt
     5041 aaataatata atagatacaa taactggttg tatgacacct atgataccag cccttacagc
     5101 atctgggatg ataaaagtta tattatctct ttgtaccact tttaattggc tatcatcaaa
     5161 ttcaagtaca tatagagtat tagatataat aggagatgta gcattttact ttatgcctat
     5221 aatacttgca ataaatgcag ctaaaaaatt taatgttaat acatcaatag cagttatagt
     5281 agtaggtgtg tttttacatc caaatttctc tgcatgggta tcaagtgggg accctatatc
     5341 atttatagga gtaccaattc aaggtgttat atatgcagca tctgttatcc ctgcactttt
     5401 aacagtatgg atgatgtcat atatagaaaa attcattaat aaattaacac ctagcatgtt
     5461 aaaaacaatt ttaaatccaa ctttagtatt attaataagt gcgccaatag cattaatagt
     5521 aattggacca ataggaaatt tcttaggaga gggattagct agtataataa atttactaca
     5581 aggaaggtta ggatttataa tggtttgttt attagcagct gcaatgccat ttatcgttat
     5641 gacagggatg catcatgcat tgactcctat attcatatca gcatttgcaa ctacaggaca
     5701 agaatcgcta atattaatat ctcaagtttg tgctaattta gcgcaaggtg gcgcgacatt
     5761 agctgtagca ttaagaagca aagatagtaa gatgaaacag ttagcatcag ctgcatggat
     5821 atcagctatc atgggtataa ctgaaccagc tctttatgga gttacattga aacttaaaaa
     5881 acctgctatg ttagcatcta taggagcagg aatcgcaggg ttatttgcag gaattactca
     5941 tgtgacttta tatgttccac aaaatagtat aatggcagta cttggattat caggggaaaa
     6001 gggaatttca aatatagtaa atggacttat agttatattt atagcattgg ttgctccatt
     6061 tattatgaca tatgtttttg gagttgaaaa aaatgttaag gaaagtacag ataatgtagt
     6121 agaggaagta taatattaac tatttacatt aatagaattt ttattattat tgactataac
     6181 ttgttttgta tttatattga tgcaaagaaa taagttaata ttaatgataa taattattat
     6241 aactgacaaa aagcaacaca aaagattgat gaagtatatg gaaataaaaa gttgacaaaa
     6301 tatgtctgat gttttatcat attaattgga attaggtagt aattgtttaa gtacaagcta
     6361 agcttattca ttaataatag cagtatccta ggaaggagta tgcccattat tatggataag
     6421 ctttttttat gcttaaaagg aggataatca tgataattaa taagatatta aacaataatg
     6481 ttgtaatcac attagatgat aatgatgaag aagttatcgt tatgggtaaa ggaattggtt
     6541 atcaaaaatc aaaaggcaat ctaatagata aaactaaagt aaataaagta tttagaatat
     6601 caaataaaga aatatcaaac aaactacaag aattactcaa taatattcct atagaacaca
     6661 tgaaattatc aagtgaaatt atagaatacg cacaaataaa gttaaacaag aagctaaatg
     6721 aaagtattta catatcctta agtgaccata catattctgc aattcaaaga atgaaagaag
     6781 gaataaatgt aaaaaatgca attctttggg aaattaaaag attttataaa gaagagtttg
     6841 aaattggaat gaaagcacta gatattattg aaaacaaaac aggtataaaa ttaccagaag
     6901 atgaggctgg atttatagcc tttcatatag tgaatgctca gttaagtgaa ggtcatactt
     6961 tggcatctga tattacgaag ttaatacaag aagtacttag catagttagg tatcatttta
     7021 ggatagaatt tcatgaagaa tcagtgtttt attatagatt tataatgcat ttaaagtttt
     7081 ttgcgcaaag attgctttta gatagtgcac atgaaggtga gacagacaaa gaattactaa
     7141 gcattattaa atctaaatat aataaagagt ttgaatgtgt agttaaaatt aaaaatttca
     7201 ttaaaaaaca atataactac atattaacag atgatgagct tatatattta acaatacatt
     7261 tagctaaagt agttaaagac tcaaatatgt aagtgaaata atttttcagt agtatattaa
     7321 gaatgattga aaggagtgat actaacaaaa ttatacctat ggttggatgg taacattgag
     7381 ttggccaaac cttcaaattt caaaaaatta atttaaacga aatatggagg agaaaaatgg
     7441 gaaaatacaa acaattagca caagagatag taaacaatgt tggtggaaaa gaaaatatta
     7501 gtggattagt acactgtatt acaagattac gttttacttt aaaagatgaa agtatagcaa
     7561 atgacaatgt attaaaaaac atggaaggta ttgttactgt aatgaaaagt ggaggacaat
     7621 atcaagtagt tataggaaat catgttgaag ctgtctataa agatgtagtt gaaataattg
     7681 gtttagatga ttcaagtaca agttcagaaa ctaaaaaatc aggtaatata ctagataaag
     7741 gtatagatat aatatcagga atttttcagc cagtgttagg aataatggca gcttgtggta
     7801 tgctgaaagg atttaatgca ttatttgtag caatgggatt atatagtgat gtatctggtg
     7861 gttatatggt aataaatgca gcaggagatg cattatttac attccttcct ttgttcttag
     7921 gatatacttc agctaagaag tttggattaa aaccaatgtt gggattagca ctaggagctg
     7981 cagtatgtta tcctgctata caaggtagca gtatatcagc aggagcagat gtgatgtaca
     8041 ctttatttaa aggaacaatg tttgcatcac cagtatatat tgatttcttt ggactaccaa
     8101 ttgtatcaat tgattatact ggaacagtcg taccaattat atttatagtg tattttgctt
     8161 ctaggtgtga aaaattattt aataagtttg ttccagattt agttaagttt ttctttgtgc
     8221 caatgcttac attattaata acagtgcctg ttgcacttat agttataggt cctatcgcaa
     8281 catttggttc tacacttatt tcacaaattg taatttcaat aagagatttt agtccactat
     8341 tggcaggtgc tatagtaggg tttacttggc aaattcttgt tatttttggt atgcactggg
     8401 gatttattcc tgtatatata aacaatgtta tgactttagg atatgataat gttatgatgc
     8461 cattctttgc ttgtacattt gcaagttcgg cagttgtgtt ggcaatattt ttcaagacta
     8521 aagataaaaa actaaaagag atgacaatac caaactttat atctggtata tttggagtta
     8581 cagagccagc aatttatgga atattattgc ctttaaagaa accatttata ataagttgta
     8641 ttgctagtgg gattggaggc gctttctacg gattctttaa tcttagaaaa tttataactg
     8701 gaggtatggg aatatttgaa cttccagcaa tgattgaacc agatggggga atgggcaact
     8761 taatagtagc tctttctggt atagctatat caatggtcgt agcttttgta ttaactatga
     8821 ttttatataa agatgagaaa gtagaagagt ctaaaaaagt aagaaataag gggaaagaag
     8881 aaataaaaga agtcaagtct actaaattag aaagagaaat agtagcaagt cctataaaag
     8941 gtgaagtatt aaaattaagt gatatagagg atgcagcatt tgcatctggt gtactaggtc
     9001 aaggggtggc aataatacct agtgatggaa aagtagttgc tccagtagat ggtgtagtta
     9061 caaccttatt tcctagtcta catgctatag ggattctttc tgatacaggt gttgaagtgc
     9121 ttattcatat tggattaaat actattcaat tagaagggag aggttttgag gcatgtataa
     9181 aacaaggtga tagaattaca aaaggtcaaa ctatattaaa ctttgatgtt gatgcaataa
     9241 aagagttggg gtatagtact gtaactccaa ttgttattac aaattcatca caatttttag
     9301 atgtagtaga aacagaaagt aaaaatattg aattagaaga taatctaatt acagtcttat
     9361 tttagtaaaa attgaggcat tagcataagc tagtgctttg gttataattt aaggaggctt
     9421 tgactatgag ttttaaggat gattttttat ggggaggagc tactgcagcc aatcaatgtg
     9481 aaggtggata caatgaagac gaaagaggat tggcaaatgt agatgtatgt ccaacaggaa
     9541 aagatagaac agcagttatt acagggaaac taaaaatgtt tgattttgat gatgagcatt
     9601 attatccagc taaaactggt attgatatgt atcataatta taaagaagat ataaagttat
     9661 ttgctgaaat gggatttaaa gtatatagaa tgagcattgc atggagtcga atatttccaa
     9721 aaggtgatga agaaacacca aatgaaaagg gacttcaatt ttatgaaaat atatttaagg
     9781 agtgtaaaaa atataaaata gagcctttag tcactattac acactttgat tgtccaatgc
     9841 atttagttaa aaagtatggt gcatggcgta atcgaaaaat gataggtttc tatgagaaat
     9901 tatgcaatgt tatctttaga agatatagag gattagtgaa gtactggcta acctttaatg
     9961 aaataaatat gattttacat gctccattta tgggtgctgg tatttgcttt gaagaaggtg
    10021 aagatgttga aaagataaaa tatcaagctg ctcatcatga gcttgtagct agtgcaattg
    10081 caactaaaat agcacatgaa gttgattcaa ataacttagt tggatgtatg tttgcagctg
    10141 gaagtgtata tccttatagt tgtaatccta atgatgtatg ggaagctaca aagttagata
    10201 gagaaaatta cttctttgtg gatgtacaat ctaaaggtaa atatcctaac tatgcgttaa
    10261 agtatatgga gcaaaaaggt attacaccag aaatggaacc tggtgatatt gaattattga
    10321 ataaatatac agtagatttt atttcatttt catattacaa tagccgttgt gttagaactg
    10381 atgaaaatgc agatgatatg gctgaaggaa acatatttac atctgctaaa aatccatatc
    10441 tttcatacag tcaatgggga tggccaattg acccattagg tttgagaatt acactaaatc
    10501 atgtatatga tagatatgaa aagccactat ttattgtaga aaatggatta ggtgctaaag
    10561 atatagctga tgaaaatggt tatgtagaag atgattacag aattaattat ctaagagaac
    10621 atataaaagc tatgcatgat gcagtttcta tagatggggt cgatttatta ggttatacta
    10681 catgggcacc tattgattta gtaagtgctg gtacaggtga aatggaaaag cgttatggat
    10741 ttatatatgt agatagagat aatagtggta atggaagtct aagaagaatg aaaaagaaaa
    10801 gttttgaatg gtataagaaa gtaattgctt caaatggtga agatttatat taaacaagaa
    10861 atctatgtta aataagaaat ttgtattaaa taagaagagt ataaaaatta ataataagta
    10921 taatttttaa cttttttaac tatagaaaat caatacagaa ttattttaca ttgtatttat
    10981 gcaatagtat gattcacaaa taaaatttta gaaaataaaa ttgttaagaa gtatgggaag
    11041 taagtgcaat ttactttgta ataaaagatg aaaaaggcga gcttaggttt taagcttgtc
    11101 ttttttatta tgtataaact atgatacttt aaaattctat gaaaaattta tttaatagag
    11161 aaattaaaaa tttaatggta atcaaattct ttaccaatga atataaaaaa tgtctacgtg
    11221 ttataatagt ttaaactaat aaacgaaagg tagatgaatg aatatgaaat accaacatat
    11281 aatagaagtt gacaaagaac tttgcattgg atgtgggctt tgtaaaaatg attgtccagt
    11341 aaacaatata attatagaaa ataaaaagtc agtaattaaa aaacaagatt gtctaatgtg
    11401 tggacattgt gcagcaatat gtcccacaaa agctattacc ttgacaggat ttgatgaacc
    11461 tcctatagaa cttacaaata agccaaagct agattcagat gaattactaa tggcaattaa
    11521 atctagaaga agtatacgta aatttaaaga caaagaagtt tcttcagaaa ttgtaaagca
    11581 aattattgag gcaggtaggt atacccctag tgctaaaaat tcacaagatg tatcatatat
    11641 tgttttagac aataaaaagt caatttatga aaacgaagca gtgaaatttt ttagaaaaat
    11701 aaaacctatt gcaaatatag ctattaaata ttcaaaagaa gttgagattg acgataactt
    11761 tttctttaag catgctccta ttgcaataat gataattaca aaagataaga ttagtggttc
    11821 acttgcagca tcaaatatgg cattaatggc agagtcttat ggtctaggtg ttttatttag
    11881 tggatatttt tcagatgtag caaataattc gccaaaatta aaaaagcttt taggtctaaa
    11941 acgcagtaat catgttctta caacattagt aattggatac ccagatgtta aatatagacg
    12001 tactgcacag aaagaagtag caactgttag atacttataa gagagggtgg attttatgga
    12061 aaaagaatgt caacaaagaa tgcaagaaat aacatctaaa tttaaagaat gtcgcaaagc
    12121 tatctctgct atgggtgatg agactagaca attgatttta attgctctct tagagagtga
    12181 ttttaatggt atacgagtgg gagaaatcac aaaaaaaaca catctttctc gtcctgctgt
    12241 atctcatcat cttaagatat taaaagaagc agagattgtt aatttgcgta gagaaggaac
    12301 gaaaaattat tattatcttg attcaaaaaa atcacaatgg aagtcactaa cagagctaat
    12361 aaatcttata tttgttggaa tacaacatat tagtgaaaat gatataagaa aaggtgatta
    12421 aattatactt gatttaatac aaaaagattg atttaggaac taaaaagttc actttgagac
    12481 actatctttt gaactatacc catcttagga gacagttaaa atatgtaaaa ctgtataaaa
    12541 caatgggtat agttcatttt ataatggttt aaatattaag aatatattat tctaaatagt
    12601 atctttctct gttttgagtt ttatttttat actcaatagc ttcagtgggg caaccacaaa
    12661 tacatgccat acaatgagta cagctatttt tccaaactgg ctttttgttt tttaaattaa
    12721 tattatttaa aggacatagc tctacacatt ttccacagcc aatgcattta tcagtataat
    12781 gaaatccttt ggcactaata attgtcttat aaaaaactgt atttattata ccacttttaa
    12841 ttgtacctcc taagccacta ggagtaatag tttcaaattc attttcattg cgtatatcag
    12901 aaataatcct atatatttgt ttatctgctt ctttaatcat ttgttttgat gtttctttgt
    12961 ctgttgtaga aaataaagct atataattct ctggcatttt aatttctgcc atacctttta
    13021 attgccattc cttgtattta catagttttt gaatatagtt tatggatttt gctgtatctc
    13081 ccccacaagt cattacaaag taaactctgt tgtctcctag aaattttgtt tcttttataa
    13141 aatctgtaac cactattggc aatctccatg cataagtagg acatacgaat atgaatggtt
    13201 tacctgctga tattaattca tctgtttttt cctcttttat aagttgattt agtgatatca
    13261 gttcatcatt taattcttgt gcaattttac gagccacata tcggctatta ccagtaccgc
    13321 taaaatataa aatcataata atatctccct aactattttt tattttaact cttaattttt
    13381 tatttattat agtactttat tgaaatattc tattcaaatg tatgtaaaat ttagtagtta
    13441 agaaaattat aacacattag taatttttat attcattggt aaagaatttg attaccatta
    13501 aatttagtaa caaaatatca actggatttt tatataagaa gagatagtaa ttttcatgtg
    13561 aaagattgta aatataaaga tgattttgaa gggcaataag aagataaaat tgatagctta
    13621 ataaaaaata tttaactttt atattaactg ttttaaatat tataattatc tatcttgaat
    13681 aatcaaccat agaagactgt atacaaagac aaagttgtgt acagtctttt ttataaattt
    13741 aatgtctata attaaataca ctattaaaat caataagtta ttatttcaaa gtagtattat
    13801 ccaaattttt atgatggtta atacaatttg ttagccattt tatagcacct tgttccctga
    13861 taatagctcc atcaagaact atataatcac caaaatcatt actatcaaga ggaggaactt
    13921 cactataacg ctccatttga ctgtacaaga acgtaattct ttcttggtgt tgaagcaatt
    13981 gatgctctag taaaataagc ctagtatcta tatctaaatt attagaaaaa tacattctaa
    14041 gacgaaaaat atccttaggg gttctttcca ttggctcatc cttttcaagc catttcaaga
    14101 actcatcttt acctttttct gtgattgtgt ataacttttt ctctaataca tcaccactaa
    14161 ttttaatatc atatacaata agcttttcat ttaccaattt ttttagttca ggatatattt
    14221 gactatgctt tgcattccaa aactcagcta attgaaaatt gaactccttt acgatatcat
    14281 aaccagtcat aggatttcta tttaataatc ctaaaattgc atactttaaa gttcgcataa
    14341 atacacctct ttctttaatt acatacttat tataacacga tatatttagt ttgaattaat
    14401 taatatagct atgttttaat aagaactttt aaattatgag gaacaaaaaa taaataaaac
    14461 tatatataaa aatgaaatta tgtaaatata aacatgtaag tatttacatg tttatattta
    14521 catatagaaa aaattgtgat aatcttaact aagaaattct atttaataaa tttatcgagg
    14581 aggagagtaa tgaatttaac acaaaaaaga tggattatat taattgcaag ttgtttcatc
    14641 aatttatgta ttggttcaat atatgcgtgg agtgtatttg catcacctat ggcagaacat
    14701 ttaaatagtc ttgctggaac aacttttaca gctgctagtt tagcaattgt atttacagtt
    14761 acaaactcag ttggacctat tactatgatt acagggggaa tgataaatga taaatttggt
    14821 cctaaaaaag ttgtatttgt aggaggatta atttttggta taggtatgat attggcaggt
    14881 ttctcaaaag gattgcctat gttgatttta tcatatggga taataacagg tcttggggtt
    14941 ggcatggttt atggatgtac aataagtaat tcaattaaat tttttccaga taaaagtgga
    15001 ttagttggtg gtataacaac agcagcttat gggtttagtt cagtaataat gcctccagtt
    15061 gctaattgga taattaataa atatggagta acttctgcat ttaaatcaat aggtttagta
    15121 tttcttataa ttgtatgtgt ttcttctttc tttatcaaaa aatgtccagc agatttcaca
    15181 ccagaaggat gggtaccacc agagaaaaaa ggaaaagtaa gagttgttat taataaaaat
    15241 tggaaagaaa tgctttcaac acctatattt tatctaatga ttataatatt aacttgtgga
    15301 gctttctatg gattaatgtg tgtgtcatta gcatcttctc tagcaacaaa tatgattggg
    15361 atgacaagtt cagattcagc aatagtggtt tcaattttag ccttgtttaa tacattaggc
    15421 cgtattgttg caggatatat atcagataaa attggtagaa taaatacttt atcaggtgca
    15481 ttagttattt caatttttgg tttattatgt ttgttattct caagagaagg agatgtagca
    15541 acattctata ttggaataat aattataggt atatgttttg gttcatttat gggaattttc
    15601 ccaggattta cagtagataa atttggacct aaaaataata gtgtaaatta tggaatcatg
    15661 tttattggtt ttgcttttgc aggatatatt ggacctgcag tgatgaaaaa tgtatattct
    15721 gtagatggca attatcaaag ggctttcatt atagctataa ttatagcttt aataggtttt
    15781 atattaacat ttttatataa atatataaat aaaatacaaa atttaaagat agaaaattta
    15841 ataaagggag atataaaaaa tggaaagaaa atatccaaat ctatgtaagc caattaaaat
    15901 tggaaatgta acattcaaaa acagaatgtt ttcagcacct atggggggaa cagacataac
    15961 agcagattgt acaattggct caaaatcaac agccttttac gaattaagag caaaaggtgg
    16021 agcaggtgca gttacaatta gtgaatgtat ggttcatcca gaaacagatg gttcacatgc
    16081 atatcatcta gacttaaaaa tagttgattc cttagcaagt tttacttata cagcagatgc
    16141 aattcgccgt catggagcaa taccaagtgt agaactatct cattcaggac aatattcagg
    16201 aacttattta gttgacaaag ataagaagcg aggattatca caatggggag taagtcctag
    16261 tgttcgtcct gatggattag agataaaaga acttacagaa gaaataattg ctgaaatagt
    16321 agagtcatat ggaaatgttg cagcattggc taagagagca gggtttgaga tgattatgat
    16381 tcatggtgga catggctggc taatcaatca attcttatca ccttacttta ataaaagaac
    16441 tgacaaatat ggtggttctt tagaaaacag agttcgtttt gctaaagaag ttttagatag
    16501 tgttcgtaaa gcagtaggtc caggatttcc aatagagttt aggatgagtg gttctgaatt
    16561 atttgaggga gggtatgatt tagattatgg aacagaaatt gctaaattat tggaatcaag
    16621 agtagattta cttcatgtat cagcaggtac ataccaaaga gggtttgcag taacacatcc
    16681 ttctatgttc ttagaacatg gatgtaacgt atatcttgca gaagaaatta aaaaacatgt
    16741 aaatattcca gttgctacta taggagcatt aaatgaccca gaaatgatgg aagagataat
    16801 tgctagtgga aaagcagatg ttatttacat ggctcgtgca ttactagctg accacgaatt
    16861 accaagaaaa gttatgagta atcaagatga aaaaatagtg aaatgcctaa gatgcttcac
    16921 ttgtatggca gaacgtgcta ccacttctac aagacgctgc actgttaatc cacttattgg
    16981 acgtgaatta gatggaactg aagttgtttc agtattaaaa cctaaaaaag tattaatagc
    17041 aggtggtgga ccaggaggat tgcaagcagc cattacagct gtaaagcgtg gacataaagt
    17101 aatactttgt gaaaaaacta atgaattagg tggaatttta aagggagaac aagcacttcc
    17161 atttaagtat aaaatgtatg aattaggaaa tacttttgga aaaatagcaa aagatttagg
    17221 tgttgaagta cgtttaaata caacagttac aaaagaatat gttgaaaatg aaaatgtcga
    17281 tgcattaatt atagcagtag ggtctgaacc tcttgttcca ccaatagagg gtcttgatgg
    17341 agaaaatgta gtaatagtta atgattatta cgtagaaaaa gaaaaagtca ctgacgaagt
    17401 aatagtttta ggtggaggat tagcaggatg tgaggctgca attcacttag ctcaagaaga
    17461 gaagactgtt cacttagtag agatgagagc agaacttgcc ccagatgcaa atataaggca
    17521 tcgtccaata ttattagaag aaatagaaag acaaggtata tatgtatata cagaacataa
    17581 gggtttaagt gtaacaacag aaggtgtagt atgtatgaat aaatctggga ataaaataaa
    17641 tattccagga acctcagtaa tatgtgcact tggacaaaaa ccaagaagag aagtggtaga
    17701 cactttactt gattctgctc catatgttgc tcaaattgga gactgtgttc gtgcatctac
    17761 aataacaaat gcagtatacc aagggcatca tgctgcgcta gatatatagt taaaaagacg
    17821 tagatagtat tgttttatta taaaaaagga gtgtctcaaa atgaactttt tagttcatga
    17881 gacacttttt tacatgaaat taaatatatt tttatcattt caacaatgaa aaaagattat
    17941 ctttattttg agacagcact tttgttatta tgattttaat tcttattgca agttttcatt
    18001 tgattgatga gtaaaacttt ttaagatgta gaagtattta acaatgtaaa tgaccctaac
    18061 ttaaatactt tagcgaaggt tgacagattt gtgtttgctt ttatctatta taacttaagg
    18121 aaacatgtta cctgaacaat aaatttatat aataaaagta tataaatata tatatgaatg
    18181 ttaagattag ttgatagaat ttaaataata aatccaatat tataaacata ggaggtatga
    18241 aaaatatgat taatgagaat ttaaaaagtc taagaaaaat ccaccagtat acacaagaag
    18301 aattagctga aaaacttaat gtttctagac aatcaatagc aaaatgggaa agtggagttt
    18361 cccaaacaaa aggatattac tgaaataacc tgtgtttacc tgcttgtaga gaaaagaata
    18421 ttgctattaa gccagagata ctacataaat gtgttggtat ctctttttcg ttaatgcaac
    18481 acagtaaatt atttcatttt cctaattatt ttagactgat aagtaacttt ttaaatgaag
    18541 aaaagaaatg acaatactta aaggagcatg gtatacttaa aggagaattt cattaaggag
    18601 gaaaaagaga ttgtttaatc taatagaaat gttatttcct ataatgttta tactgatttt
    18661 tataataatt gttttcactt ttgtgaaagg aatagctact tggtttaaaa acaataattc
    18721 tcctcgctta actgtgtcag caagaatagt agcaaaacgt cagaatacca ctcataacaa
    18781 tcaaccaaac gctggtgata tatcaggagc acatggattt catacaattt ccagcacctc
    18841 gtattatgtg acatttcaag tagatagtgg tgatagaata gagctgtctg tatctggctc
    18901 agagtatgga gtgcttgcag agggagataa aggacagctg acattccaag gcacaagata
    18961 tttaacattt gatagggaaa attgaattta gaggtgcatt atgaataagt ttttgagtca
    19021 gataaggcgt atagataatg aaatatctat tactaggaaa attataaata caatatttat
    19081 actgtgtttt ggaatagttt taggaacttt tgcaaaattt ttagatacta cagcaagcaa
    19141 tactttacca tttatatttg aatatttaga tattagtaat ttttttggtc gttttgctat
    19201 atggttattg attgctttgt acattgcaat ttatagtcat tcttccataa gagcatcatt
    19261 aaatgtgtta gtatttttca ttggaatggt ttcaagttat tacctatatt cgtattttgt
    19321 tgctggattt tttccaaaaa attatgcaat gatttggctt ggttttactg tcatttctcc
    19381 attgttagct tttatttgtt ggtatgctaa ggggaaaagc aaaatatctt ttatactttc
    19441 agtaataata attgcaattt tatttaattt tacatttatt tatggttgga tttattttga
    19501 tgtgtattca attttagaag tgatagtctt tggttgtgca ttaatagctt taaaacgtaa
    19561 cacctttagg gagacaacgt atatgatatt gagtgcagtt gttattgctg ttatacttaa
    19621 tatgttagtt ccatttcatt ttggttaggc agattttaag attattatag atagaatata
    19681 aatttcacga accgccgaag cgatccgcca gcggtggcgg tcgtaagaca tatcgccacg
    19741 ccagcttgct ggcgaggtgg cggttcactt tggcggtttg gcgagcctgc gagctttcta
    19801 agcccccgtt atctaacagg gggggactat tacaaaagac aagagcaaaa acaaatataa
    19861 aactgctgat atgcaacgat ttataaggtt gtaagaactt atttcacttt acaccctatt
    19921 ctttctaccc gacaggtcta aaacagcata tcaatagaaa ataaatataa gatacaaggt
    19981 gtatgctcct atgagtatat gccttttttt gattggagga atgaagcatg aatgatatga
    20041 aatttattga aacattaaag caaaagagaa atgtctgtga ttattcgcaa tcacgactgg
    20101 cacaggaact acaaatcaac aggcagaact taaatgaaat tgaaaacgga aaaacaaaag
    20161 ccagtaaaga aatgaaacat atactcttac attatcttga ttattgtaat tgtacacagc
    20221 ctttcacttt aacgattgac tatctgcgtg ttcgttttcc taccacagac gcattggaaa
    20281 ttataaaaaa tgtgctggca atgaaaagcg aatattttat acatgaagat tacggaatgt
    20341 atgcttatga agaacagtat atctatgggg atattagtgt aaatgcttct aaagatagtt
    20401 ctatgggtgt tttattggaa ttaagaggta tggggtgtcg ggacttagaa tatgttttac
    20461 aggtaagagg gatagactgg tattgctttt taagatgttg tatagattat caaggtgttt
    20521 ttaaacgcat agatttagca gtcaatgata tgggcggatt gctggatata gaaattctaa
    20581 gggaacgtta ttatgctaac aaggtatgga aacgttcaag aacccatgaa gcagtagaca
    20641 gcggaaaatt atcgggtaca catggagata ctgcaaaaac tttttatatc ggttcaaaga
    20701 atagttctat ctacttttgc ttgtatgaaa aagaaaagga acaaaaaagc aagggtataa
    20761 aaacagatat taagaaccgc ttcgaaattc gtttaaaaaa tggaaaagca ggacaaacgg
    20821 tagagcaact tgttttttca agaaatcccg aacagaccat agcaagcttt attctcacac
    20881 agatagattt tcccgattat attttatggg atatattttt agataatgta acgacctcac
    20941 ttccgtttat tatgacacct gttgctgtta atatggataa aataaaacgc tggctagaaa
    21001 gacaggtcat gccctcttta ttgatgataa aagagattga aaagaaaaca ggagcaaatt
    21061 atctggaaga aatcgacagg catacaaggc taacagaaaa gcagtaatta aagattaaac
    21121 aaatgacaac agacatagca gatatgattg agaaagatac cgctgttcca caagggaatg
    21181 acggtatttt ttaatttctt caaaaaaact gcaacaaaat gactgcttgt gtacagatat
    21241 ggtgcgagga aaggaaatct aacttttttt gaaaacaggt cattaaatcg gtggctgctg
    21301 tccctatatg atgtgaaaga agaaacgatt tttaatttca acttaacatt tgacagtttt
    21361 cattccctat atggagcaaa gaaaatcttt tattccatag ttggcagtta cggtgttgca
    21421 tgggtagtta gggtgtgaaa agaaaaacag atttttttca tcaccaactt agcaaactga
    21481 tacttgcttt ccctatatgg tgcaggaaaa gagagtttaa ctattttcat ttcaacttag
    21541 caactggacg tttgctgtac agatatgtag tgaaaagaga aaaagttttg aatattggtt
    21601 acgcttcggc tcttttccaa cgcggtatat aggaaagacg attttcttct ttcatgttct
    21661 ttgacaactg aataaagcaa ttcaatacgt ttgacagaca gcgatccatt gaagcagata
    21721 cgccatgacc ttttccctgc aaaagcgagc gaaccctatt tgtgttcctg taaaagactt
    21781 gtcctctttt atcgccatga cctgttctgt ctgataaatg atactcccgt acagccacag
    21841 cttgaacgtt cagagcgtcg cacgcaatgg gtacggctac ataagaacta tgcagaggtg
    21901 gaattcctgt gggctatgac aaatgccgtt gaattgctta gaaaaaagat tatggaaagg
    21961 gggtatgtgt tattgctgat attgtaaaaa caatgcaaga aaagacaaat aataagcgta
    22021 gcattggcaa cagaggaaaa acaaagattg ttgtaaaaga gcatttttcc gaaaagggaa
    22081 agacaatgga ggaacttctg actgatgtta tgctggaaaa agcaaagcag acaatcgcat
    22141 aaaattggtg cagttgtaag acatagattg aatggcaaaa agtacccgtg ttatacttta
    22201 cttgcaagca ggtattgtaa acacgggtta gttataaagg aggataactg acaatgaaac
    22261 aacagattta caatactgca ctttatctta ggttaagccg agatgatgaa ttacagggcg
    22321 aaagttccag cattaccaca caaagaagta tgttgcgtct atatgcaaaa gaacatcatt
    22381 tgaatgtgat tgatgaatat attgatgacg gctggtcggg aacaaatttt gaaagaccga
    22441 gttttcaaag aatgattgag gatatagagg caggaaaaat caactgtgtt gtaacgaaag
    22501 acctttcccg acttggcagg aactatatta tgacaggaca atatacagaa ttgtattttc
    22561 ccagccataa tgtccgttac atagcgattg acgacggtgt agacagcgag aaaggcgaaa
    22621 gtgagattgc accatttaag aacatcatca atgaatgggt ggcaagagat acaagccgta
    22681 aagtgaaatc ggcatttaag acgaaatttg cagagggtgc tcattatggt gcttatgctc
    22741 cattaggata taagaaacac cctgacatca aaggaaaact gctggttgat gatgaaacaa
    22801 aatggattgt tgaaaaaatc ttttccctag cctatcaagg ttatggtagt gccaaaatca
    22861 caaaggtact gcgagaggaa aaagttccga cagcgtcttg gctgaatttt acaaggtacg
    22921 gtacttttgc acatatcttt gagggaaagc ccgaaagcaa acgttatgag tggacgattg
    22981 ctcatgtcaa ggcaatctta aaaagtgagg tttatatagg caacagcgtt cacaatatgc
    23041 agtctacggt ttcattcaag agcaagaaga aagtgcgtaa gcccgaaagt gaatggtttc
    23101 gagtggaaaa tacccatgaa ccgattattg acaaagaagt gttctatcgt gtgcaggaac
    23161 agataaaatc aagacgtaga cagacaaagg aaaaagcaac accgatattt gcagggcttg
    23221 tcaagtgtgc ggattgtggc tggtctatgc ggtttgcaac gaatacggca aataaaacgc
    23281 cttacagtta ttatgcttgc agttactacg ggcagtttgg caaaggtact tgttctatgc
    23341 actatatccg ttatgatgtg ttgtatcaag ccgtattgaa acgattgcag tattgggcta
    23401 aggcagtaca gcaggacgaa gaaaaggtgc tgaacaagat acagaaagca ggcaatgcag
    23461 agcgaatacg ggaaaagaag aaaaaggcaa gtgcctttaa gaaagccgag aaccgacaaa
    23521 atgaaattga ccgtttgttt gcgaaaatgt atgaagatag agcctgtgag aagataacag
    23581 agcgaaattt tgtgatgttg tctagcaagt accaaaaaga acaaatagaa ctggaacagc
    23641 agataaccaa cctaagagaa gaattaagta aaatggaaca ggatatgata ggtgctgaaa
    23701 agtggattga gttaatcaag gagtattccg taccaaagga actgacagca ccgttattaa
    23761 atgcaatgat agaaaaaatc cttattcatg aagcaataac gaatgaggat aacgaaagaa
    23821 tacaggaaat agagatatat taccgattta ttggaaaagt agactgataa agagggttca
    23881 tatctttaac taagggaaac tggagaatct attcctgata taggtagttg cattaaactt
    23941 gcaaaattgt acaaggtcaa actagatgac ttagttaatc attcggaaga aaaaacaggt
    24001 attatagtac ctcctaaagg aaagtttttc tttggagcag tagtagttgg tgaacgtggt
    24061 cagattgtca ttccaaaaga agcaagagaa gtatttaata taaatgcagg agataaatta
    24121 ttggtacttg gagatgagga aagaggatta ggaattgttc atcaacgtga tttgataaat
    24181 tttataggtc atatgggagt agtacgtaaa gaagaatgat tttaaaaaat tataaaagga
    24241 ggtatgaaat gtgagtaaaa tagtattttt tagcattctt gcatatggtc atactaatcc
    24301 tactattgaa gtagttcgtg aattagtgga tagaggaaat gaggtattgt attattcttt
    24361 taatgaattt aaagataaaa ttgaaggtgc tggagcaaaa tttatttgtt gtgataaata
    24421 ccttccagaa ttactccctg gagatgagaa aaaaattggt aaggattttc ctggattaat
    24481 tgagatgata gtagatacta ctatttcttt agatgaaaaa gtttgtaggg aacttaaaga
    24541 ttttaatcca gattgcattg tatcagattc attaagtttt tggggaaagt tatttgcaca
    24601 aaaacttaat attccatatg tatgctctac aacaactttt gcatttaata aacatacagc
    24661 aaagttgatg aaacaaaact ttatagaaat tatacgtatg atttttggtg taagacgcat
    24721 taataaaaaa ataaagcttt tacaaagaaa agggtatgaa gtaaaaaatt ttatttctat
    24781 agtttctaat gataatgata cggataccat tgtatataca tcaaaagaat ttcaaccaat
    24841 ggtagaaacc ttctctagta agtattcatt tgtaggtcca tcagtatcaa agttgattat
    24901 taaacctaaa gagagaaaaa gaaagttaat atatatttcg cttggtacaa ttaataataa
    24961 aaatgttagc ttttataaaa actgtataaa tgcatttaaa gattctgatg ttgatgtgat
    25021 tatgtctgtt ggtagaagta ctgatattaa aagtttagga aatataccaa ataattttga
    25081 ggttaaaaat agtgttgaac agattgctat acttcaaaaa actgatgtgt ttattactca
    25141 ttctgggatg aatagtgtta atgaaagttt atattatgga gtacccatgg tattatttcc
    25201 tcaacattcg gagcagagaa tggtagctaa gagagttgtt catttgggag caggaattat
    25261 gttaaaagag gataagtccg aaagtattaa aaaagcagta tttcaagtta taaatgacaa
    25321 tgaatataaa gaaaatgcaa ctaaactatc aaaaagcttt tataatgcag gaggctcaaa
    25381 aaaagcagca gatgtaatat tacagattat atgtaatagt agacaagttg acatataata
    25441 gatttgttaa aaatatatat acatacatgc aatttaatca aagtactgac agtggggtct
    25501 tttaacctac cagttggggc atatatattg atgctaaata tgagggatgt tatattttaa
    25561 taaaaaagat aacatccttt ttttagtgcg ttatatcaag ttttaaataa taaatgaagg
    25621 aggattaata tgaaaaaata taaagttatt tttatagtat taattatagg tattataagt
    25681 gtaacagggt ttgttgtata taaaaactgc attaaaccat ggaaagatgc tgaaacatat
    25741 attgataagt atatggtaaa acaagatatt tcaaaagata atataaaaag tattacaaaa
    25801 gaaaaagcca agaaggcaag ttacgaagga atactataca aagtgtatta caaagatgac
    25861 cctcgatatg agtatcaata tttttatagt gataattatc atgctctata tgtaaataaa
    25921 gtaatgttac aaatttatga tgtggaaaat aataataaac ttttaagaag taatgaggaa
    25981 ttaaaaagtg taaagtatcc accaatatat cttaaatata agccagataa atgatttatg
    26041 acaaaataga atgattttta ttaaaattta tttaaaaata tcactagagt gtttaaataa
    26101 aactaaattt aagtatttat a
//
LOCUS       DAMJVX010000036        26093 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMJVX010000036 DAMJVX010000000
VERSION     DAMJVX010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.038"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            153..890
                     /gene="cas6"
                     /locus_tag="PC407_001423"
     CDS             153..890
                     /gene="cas6"
                     /locus_tag="PC407_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2496875.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            895..2370
                     /locus_tag="PC407_001424"
     CDS             895..2370
                     /locus_tag="PC407_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496876.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKKMIDNGNLSNLKELLLDNISVLRQLTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2390..3403
                     /locus_tag="PC407_001425"
     CDS             2390..3403
                     /locus_tag="PC407_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HDF2496877.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIIFGKKGNIYPSIKQNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEKEENEVLR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDKLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            3419..4225
                     /gene="cas5"
                     /locus_tag="PC407_001426"
     CDS             3419..4225
                     /gene="cas5"
                     /locus_tag="PC407_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HDF2496878.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIKKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVMPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCNVCIDTKEVFTWL"
     gene            4252..6660
                     /locus_tag="PC407_001427"
     CDS             4252..6660
                     /locus_tag="PC407_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2496879.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLLEEDKLYIYKSIMLHHGNYEDYMRLSTSKVQEEIYDYIE
                     KGILEKEEFNLKDIESYINDILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDDMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEGALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     NYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            7101..8048
                     /gene="prmA"
                     /locus_tag="PC407_001428"
     CDS             7101..8048
                     /gene="prmA"
                     /locus_tag="PC407_001428"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDF2496880.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQDGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            8067..8825
                     /locus_tag="PC407_001429"
     CDS             8067..8825
                     /locus_tag="PC407_001429"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDF2496881.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            8827..10125
                     /gene="mtaB"
                     /locus_tag="PC407_001430"
     CDS             8827..10125
                     /gene="mtaB"
                     /locus_tag="PC407_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDF2496882.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10168..10518
                     /locus_tag="PC407_001431"
     CDS             10168..10518
                     /locus_tag="PC407_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HDF2496883.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10681..10860
                     /locus_tag="PC407_001432"
     CDS             10681..10860
                     /locus_tag="PC407_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDF2496884.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            10890..11333
                     /locus_tag="PC407_001433"
     CDS             10890..11333
                     /locus_tag="PC407_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDF2496885.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11444..11941
                     /locus_tag="PC407_001434"
     CDS             11444..11941
                     /locus_tag="PC407_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDF2496886.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            12134..12697
                     /gene="raiA"
                     /locus_tag="PC407_001435"
     CDS             12134..12697
                     /gene="raiA"
                     /locus_tag="PC407_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2496887.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            12910..13146
                     /locus_tag="PC407_001436"
     CDS             12910..13146
                     /locus_tag="PC407_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HDF2496888.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            13159..14502
                     /gene="yqfD"
                     /locus_tag="PC407_001437"
     CDS             13159..14502
                     /gene="yqfD"
                     /locus_tag="PC407_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HDF2496889.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            14441..15556
                     /locus_tag="PC407_001438"
     CDS             14441..15556
                     /locus_tag="PC407_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDF2496890.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKVKMQEKTFKRK"
     gene            15613..16074
                     /gene="ybeY"
                     /locus_tag="PC407_001439"
     CDS             15613..16074
                     /gene="ybeY"
                     /locus_tag="PC407_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDF2496891.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            16080..16799
                     /locus_tag="PC407_001440"
     CDS             16080..16799
                     /locus_tag="PC407_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDF2496892.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            16918..17319
                     /locus_tag="PC407_001441"
     CDS             16918..17319
                     /locus_tag="PC407_001441"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDF2496893.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            17383..18276
                     /gene="era"
                     /locus_tag="PC407_001442"
     CDS             17383..18276
                     /gene="era"
                     /locus_tag="PC407_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDF2496894.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            18288..19667
                     /gene="mgtE"
                     /locus_tag="PC407_001443"
     CDS             18288..19667
                     /gene="mgtE"
                     /locus_tag="PC407_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDF2496895.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            19740..19895
                     /locus_tag="PC407_001444"
     CDS             19740..19895
                     /locus_tag="PC407_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HDF2496896.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            19861..20634
                     /gene="recO"
                     /locus_tag="PC407_001445"
     CDS             19861..20634
                     /gene="recO"
                     /locus_tag="PC407_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDF2496897.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            20627..21226
                     /locus_tag="PC407_001446"
     CDS             20627..21226
                     /locus_tag="PC407_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HDF2496898.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            21386..22264
                     /gene="glyQ"
                     /locus_tag="PC407_001447"
     CDS             21386..22264
                     /gene="glyQ"
                     /locus_tag="PC407_001447"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDF2496899.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            22264..24330
                     /locus_tag="PC407_001448"
     CDS             22264..24330
                     /locus_tag="PC407_001448"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDF2496900.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIRLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(24496..26043)
                     /locus_tag="PC407_001449"
     CDS             complement(24496..26043)
                     /locus_tag="PC407_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HDF2496901.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
BASE COUNT        10659 a         2576 c         4714 g         8144 t
ORIGIN      
        1 tttagcaaag aataaaacta acagtaaaat atatttaaaa aattaaaaaa caaaattaaa
       61 tttattatca aaaaatcatt ttatgatatg ttttattttt tatattacaa atagagaata
      121 atgaattata ctagaaagta ggaggtgaaa atatgagcag aatttcaatt tttatggagt
      181 catgtaatgg caaatttcca aatgaaaatt caatgttgtc agtttccttt ataaaaaata
      241 ttttaaatgc agaaaacaaa gattttgcta aaaatatatt taactatggg gaagaaagaa
      301 aaaacaataa gcaaataaag gatataaata cagccatcta cataccaaat cttataagaa
      361 gcaaaaatga gttgcttgtt gatggagata taatattcaa tataagtttt tataattatt
      421 ctatgttcag taaattatac aatggaattt taaaagtaaa ggagctagaa tataaaggtt
      481 atagatttaa aataaaaaat ataaaaatac ataaagaaca cgagataaaa gaaaatggtg
      541 ttatatttaa aactatgtct cctataatag taaagaataa agaaggtaaa tatttagatg
      601 tagaagattc aaattatata gaatgtttaa actatatagc taatctaact ctggagagta
      661 taagaggtag tgggcttaga aagccattgg aatttatacc tcttaacttc aaaaaaagag
      721 tgttaaaaga aaagattcgt ggatttaaag tgagagaata ttactatata aatgcttatg
      781 caggaacatt ttttttaaaa ggaaatatgg aagatttaaa tgcactgtat aaaatgggaa
      841 ttggatatag aaggacggaa aatgcaggaa tggttgatat attaaagtag cgaggtgaga
      901 gaaataaaag ctaaatttta tagatcaggt gatattgtaa gagatattgg cattgtttac
      961 ttttatgaat tactaatgga tttaaaaaac gaactagaaa aaaatgatta taagctaaaa
     1021 tttacttgtg agttaaatag aaattattta tctttagaat ctgcagaata tatagaccct
     1081 aagtatatat ctgattatat attagaaaat caacttttta aggtatttaa aaatgggaaa
     1141 aaggaaacct tagaaaaaat atttagtaat atagataatt taacgtatgt aaattttatt
     1201 gaagaattag ataaatcttc tgcaactgag aaacaaaagg aagggattaa aaaagatttt
     1261 aaaaacagaa gatttccata tgttagaaat agtggtaagt atggattaaa cacgagttta
     1321 gaaaatatgg aaacaaatgt aaaaagaata gtagatactg taattaaatc aaatatagag
     1381 atagatgaaa tagataaact tgatttgaca caatatgaag aaaaagatag tgtatgtaat
     1441 gtttgtaata taaataaaac aacaaaacta gatattgatt taagagagcg aaaggactct
     1501 aagtataact ttttatttag aggtgcagaa aaatcaggct ttaaaagaag tggacaagtt
     1561 gaaagtaata tttgttttga gtgtgaattt ttcaacttaa tgtgtctttt atatataaat
     1621 ttaaaaagac caatggtttt tgcatatacg aatgatttaa gggaattagc atttttaaat
     1681 cataagataa tgcttaaaag aaagatgtat tcagataaat cattttataa aaaacttttg
     1741 catgaaaaaa taagttcgat tagactatat aggtttgata ttgatacgaa taaaggtata
     1801 attctaaagt ttgactctat tatagagtat aaagaattat taaaaaaaat tgaacttata
     1861 gatattatag ataattataa tttttcaaga gagccaggga atactagaaa tttgggtaaa
     1921 aaaatgattg ataatgggaa tttatcaaat ttaaaagaat tattacttga taatataagt
     1981 gttttaaggc aactaactgg aacttctcga gaaatggatt ttgtatctag ctcatataat
     2041 atcggtttat atataaaatt gtgttggatt gtagatggag gggaggagta taaaatgaaa
     2101 aactatatgg aaagtaattt aatatattca agagtaggta aagacttatt taataaaatg
     2161 acagatgaat ccagaagaaa aaatttttct atgaaagtga ttcaactact taagtctaat
     2221 gatagaactc agctatttca aactataatg catgtgttag tttctaatgg aatcttaata
     2281 ggagaagggt ttgtagaagg tctaatgcag agttctgaat tagaattaaa tacgaatgta
     2341 ggtctattta ttcaagaaat catgaaataa aaaaggggga aaatttaaaa tgaatgaaaa
     2401 aagagtaaaa ggagtaacat taactatact gacagaaagc ccagttccac tttcatatga
     2461 ccaagggtat ggaaattata caccaataaa aaaagaacaa tatagagaaa aaatacatgc
     2521 aaaaactagt atagccacaa taacatatga tttaagaaga atgctccacc aagaatatgg
     2581 ttggaattta agtaatatta tttttgggaa aaaaggaaat atatatccat ctattaagca
     2641 aaatgtagga tgtgtagatg aaaatggatt agagactgat gtatttggtt atttgatacc
     2701 acttaaagat aaaggttcta taagtaaggc ttctccgctt agaataatac cttttaggtc
     2761 attaaattca tatagaggtt caacacagtt gataacaaat agaggttttt taagtagcga
     2821 atttggtaga aaatattatg atgagaagga agaaaatgaa gtacttagag atgaaaactt
     2881 tccaacatca caagcattag caatagaaga gactttatca gattactacg tttatacaat
     2941 aacattggag cttgatagaa taggagtagt agaagtagaa gatggaaagt tgcttttacc
     3001 agaagaaaga aaatttatga gtaaggagtt aagagaaaaa gcagtaaaag atatattaga
     3061 tgcaattact gtgtttacaa gaaacataaa acattcatca gtactgttaa aaccacttgc
     3121 agtaatgggt ggagcttttg ataaagttgt accgtttttt tgggatgatg tagattataa
     3181 tgctgatagt ggtgagataa atttagaggg agtcatagaa actatagaaa gttatagcct
     3241 taaagaaagt aacacaatac ttgctatcaa tgacaaatta aaaatttcta ataaaaagga
     3301 attaaataag tttaatttaa gtaaataccc tgtaaaagag attaaaaatc tagcagatag
     3361 attggaaatt ggagaagata atatgtggta tttaaaagag taggaggttt tttgaattat
     3421 gaaagtatta caatgtaaac ttaagcaacc aactgctcac tatagagacc caaaagtatt
     3481 tcaaaatgaa tatataagta ctttaaattt accatctaag accacaataa tgggaatgat
     3541 aacgtattta tgtgatagaa gattaaactc tgatatagat ataggaataa ttggaacaca
     3601 tcatcataga gaactagaat tttcaagagg agagaacatt gatttttgga atgaatatac
     3661 aaatatgaga aaaggcaaag ataaagaaaa gtttttgctt caaggaaact actatgatta
     3721 ctataaagaa cataaagctc aaaatagcat attaaactat gaagttctta aagaagtaga
     3781 actgactata tttatttctt gcaaggatga tgaagaactt gaatttatta aaaaaaagct
     3841 tgaaagtcca tgtaaatatg caaatttagg aagaaaagaa gattttgtaa taccaagtga
     3901 aaaagggtgt tttgtgaaag aagttgagtt aaaagaggtt atgcctatga atactcgtga
     3961 tgctataaaa gaaaatataa aattaaagaa tacatatgta agagttgact taagagataa
     4021 agagaatgtc gagactataa taaatcaagg tgtattgatt gcattacctt ataaatataa
     4081 agatttagaa gcaaatagag atgatagagt ttatgagttt tgccattata tatatgttga
     4141 taatgatggt atatatccta aaaatattaa ggtaaatgta tgtaatgtat gtattgatac
     4201 aaaagaagtt tttacatggc tttaaattat tttgaaagat aggtgtttat catgaaaaat
     4261 atcatgcaaa aagaaatatt agctaagtca tataatttaa gaactaatca acaagaacaa
     4321 acattagttg aacatattaa ggatttattt gaagtattag aatctatatt agaactaaat
     4381 ctatattcag ataaagatgt agagatacta aaaatatgtt gtgctttgca cgatttagga
     4441 aaaattaatt caattatgca acaaaaaatg gagattaata ataaaatatc gtatagttgc
     4501 tcagaagaag agaggaaaaa gttagagtct gacaaaaaat ctttaaaaaa gatagcaaga
     4561 cacaatatat tttctggagc atttttaaaa gatatattag aaaaaatgaa cttgttagaa
     4621 gaagataagt tatatatata taaaagtata atgttacatc atggaaacta tgaagattat
     4681 atgaggttaa gtactagtaa agttcaagaa gaaatttatg attatattga aaaaggcatt
     4741 ttagaaaaag aagagtttaa tctgaaagat atagaaagtt atataaatga cattttaaat
     4801 gtagatttta aatttggtga agatgtattg gattatgatt acatagataa attaagtgaa
     4861 agtatgttta tagacagtga ttataacaat ggtcaaatag atgattcagt tttaaattat
     4921 agaaagttta agtatatttt atataaaggg atgctgaact taatagacca tagtgcatca
     4981 agtagacaga aaggtataaa attttataac gattttactg atgaagaaat tgatgatatg
     5041 attttaaatg agatatataa gagtcagggt aatttagaaa aaaataatat agagttcaat
     5101 actatacaaa aaaggttgag agaactttct ggaagaaatg ttctgactgt tgcatttaca
     5161 ggttcaggaa aaacagctgc agattataga aagactttta aaagaaaatt ctttttagtg
     5221 ccaaataaaa tatcagcaga aagtttttat agaaaaaata tttttcaaaa taaaaatgat
     5281 ttagatacaa gaagtagtaa tgattacatt ggattacttc atggagatat aaacttatat
     5341 tcagagaatg aagataatgg agaacatgat tttgtattga cattaaggga tatagatttg
     5401 agtataaatt tttgtaaacc ttgtgtaatt gcaacagttg accagttatt acttagtatg
     5461 tttaaatttc cgggatatga aaaaatattc gcagccgtaa aaaatgcatc tataactgtt
     5521 gatgaagtac atttattgga accaaagatg tttttgattt tgatatattt tatgcagttt
     5581 gcatgcaagt acttagatgt tgattttcac ttaatgacag ctacacttcc aaaatcatat
     5641 aaagagcaaa tgataaacaa gggaattatt tttcaagaag aaagtaatga aaatgttact
     5701 gataaaggag aaatagtttt tattgagagc aacaaggaag aagaaatttg tgaaggtaaa
     5761 gatgtaaagg tttcttttat aaaggaaaaa gaaatcaagt caatagttga aggtgcttta
     5821 gaaaataaac aaaaaattct tataataaag aatactattg attcagtcaa tagaacatat
     5881 gagtttttaa aagaaaattt atctgataag tatggtggtg tagatataga tgttttacat
     5941 agtagattta agttcaaaga taaaaaagag aaatatagta aaatattaaa tggagagggt
     6001 gatatttgga tatcaactca atcagtagaa atatccttgg atttggattt caatataata
     6061 ataagtgatt tagcaactat ggatagctta atacaaagaa tgggtagatg caatagaaat
     6121 aataaatatg aatatggaaa cttttatatt ctacctagtg aagataaaat ttatgataac
     6181 aaacttaaga acacaactaa aaacatctta aaggacatat taaaaactaa ctctattttt
     6241 acaatgggga ttagaaaaac tattttggat gattactatg ataatagtgt agttaaaaac
     6301 tattttgaag agaattttat aagttgtgat aaagaaatta agaatattta tggtataaat
     6361 aaagaaattt ttgatggatt ggatttaatt tttaattttg aaccttacaa aaatattgtt
     6421 gatagtaaga aggaagcagc taaaattttt agagatgttg atgttagtta caaaattatt
     6481 ctggaagaag atttttataa ggaagatagg gagttacaac aagattcgat tcaagtatca
     6541 gggtttatat ttaatagatt atattattat ggattaataa gcaaagtaga aggatacatg
     6601 gtactaaaat ctagtagtaa gtttgagtat aatagtactg taggcttaat tttgaagtga
     6661 gcatatgttt aaatataata tacctttaga cattgaaaat acaaaaagta ctactgtaag
     6721 cccactgtaa aaactatttt ttaaaaggaa aaataagttg tgagatagag tgtctacaag
     6781 tgtatataga taataaattt gaggtttaag attaactaag tgggtttcag gtacatcgcg
     6841 aaaataatta taagttggag attttgtatg tttaagatta actaagtgga tttcaaatat
     6901 tgatatagta gatataatag aagttttatt attaggttta aaattaacta atcggatttt
     6961 taaatattct gactattata taaggatatt attagcttga atttaaaacc aataatataa
     7021 aacaacaaaa tattatattt atgatataat aaaagttatg gaaaaacagg aatattacta
     7081 tagagaagag aggtaaactt atgaataatt ggatagaagt aactataaaa acaacaacag
     7141 aagcagttga acctataacg aatatactat atgaacaagg agctggagga gctgtaatag
     7201 aagacccaaa agacttttta ttccaaaaga aaaatgaact agactgggat tatgttgaag
     7261 aagaagtatt taaaaagaat gaagaagatg atgttcttat aaaaacttat gtttctgaag
     7321 aaaaaaatgt gatggaattt gtcgaaataa taaagcaaaa agttttagga ttaaaagatt
     7381 ttggtataga tataggtgaa ggaagtgtat cattggacca agtaaatgaa gctgattggg
     7441 caaatgcatg gaaagcatac tacaaaccaa caaaagtagg acaaagggta gtagtaaagc
     7501 ctacatggga agattatgct atgcaagatg gggatttaat catagagctt gaccctggaa
     7561 tggcatttgg tacagggaca catgaaacta ctagtatgtg cattagagaa ttagaaaagt
     7621 atgtgaataa agattctaaa gtatttgata ttggatgtgg tagtggaatc cttgcaatag
     7681 cagcagctaa attaggagca aaagaagttg tagctgtaga tttagatgaa gtggctgtaa
     7741 aagttgcaaa agaaaatgtt cttgaaaata aagttgaaaa aagtgtatct gtaatgcatg
     7801 gtaatttaac tgatgtaata aaagataaag cggatgtaat agttgcaaat ataatagctg
     7861 atataataaa gatacttgca aaagatgttc aaaactttat gaaagaagat gctatattta
     7921 tatcttcagg tataattttg gataaagtag aagaagttaa agaaagctta attgaaaatg
     7981 gatttgaaat agttgaagtt caaaaattag gtgaatggtc agcaatagtt tctaagctta
     8041 aaaaataagg gatatggtga cgtataatgg atagattttt tgttgaaaaa aataatataa
     8101 atctacaaga taaaacttgc actatagaag gtgaagatgt aaaacatatt tcaaaagttt
     8161 taagatgcaa actaggtgag aaacttgaaa tatgtgacaa gaataataat gagtatattt
     8221 gtgagataat gaatatagat aagagtatag ttaatttaga gattttagaa aaagttgata
     8281 taaatagaga atcagaactt aaagtaaggt tataccaggg acttccaaaa gctccaaaaa
     8341 tggaaatgat acttcaaaaa ttaactgaag tgggtgtaga agaaataata ttagttcaaa
     8401 ctaagagaag tgttgttaaa gttgatgaca aaaaagaaga taaaaaattt gaacgttggg
     8461 aaagaataat ttatgaagca gcaaagcaaa gcaaaagagg taagatacca aaattaagag
     8521 gtgttttaag ttttaaagaa gctttagaag atatgaaaaa aaataatgta aatatttgcc
     8581 cttatgaaaa tgaaagaaca gtttcaataa aacatgcact aaaaaaatgt gattctaata
     8641 tagatagtgt tggaattttt ataggtccag aaggtggatt ttctgaagaa gaaatagagc
     8701 aaatacaaaa aaataattgt aatgttgtat cattggggcc tagaatatta agaactgaga
     8761 cagcttctgt tgtagcatca acaatagctt tgtatgaact aagtgattta ggaggagaaa
     8821 aataaattga aaaaagtagc tttttataca cttgggtgta aagttaatca atatgaaaca
     8881 gaagctatgc tagaactatt tgagaaagat ggttatgaac aagtcaatag tgaagagtat
     8941 gctgatgttt atgttataaa tacatgtaca gttactcata tgagtgatag aaaatctcgt
     9001 caatatataa gacgagttaa aaagaagaat ccagatgcaa taatagccgt tgtaggatgt
     9061 tattctcagg tatctccaga agaaattttg gatatagaag aagtaaattt agtcatgggt
     9121 acaaatgata gaagaaagat tgttgaagaa attaaaaaga taaattcaag taagaaagtt
     9181 agtacagttg atgacataat gaaagttaaa gcttttgaag aaatagaaat cagccaaact
     9241 aatggaaaga ctagagcatt tatgaaaata caagatggat gtgacagatt ttgtacttat
     9301 tgtatcattc cttatgcccg aggacgcgtt agaagtagag atattgatag tatagtcgat
     9361 gaagtgaaaa aattggctaa taatggctat aaagaggttg tattaacagg aatacatgtt
     9421 gcctcttatg gaaaagattt aaaggatagg gatattaaac ttcttgatgt aataaaacaa
     9481 ataaaccaga tagaaaaaat agaaagaatt aggcttagtt ctgttgaacc aattttattt
     9541 acagatgaat ttgtaaatga agttttaaag atggataaag tttgtcctca ttaccattta
     9601 tcacttcaaa gtggatgtga tgaaactcta aaaagaatga atagacgata cacaacttta
     9661 gagtacaaaa ctattgtaga tagattgaga agtaaaatgc cagatgtggc aataactaca
     9721 gatgtaatag taggttttcc tggagagaca aatgaagagt ttaaaaaaac gtacgagttt
     9781 ttaaaagaaa tagagctttc acaaatgcat atttttaaat attcaccaag aaaaggaact
     9841 ccagctgcaa ctatggaaaa tcaagttgac cctcaaatga aacactttag aagtgaacaa
     9901 cttttaaatt taagtaaagt aaattttaat aaatttgcaa ctaaatttat cggccgtgag
     9961 ctggatgttt tgtttgaaca aaatatagaa ggcaataaat atgagggatt aacttcaaat
    10021 tatataagag ttgttgttga aagtgacaaa aatatacaag gtcagatatt aaaagtgaaa
    10081 ataaatgatg taaaagatga atatgtagaa ggaattttgc tataacatgt agaaattata
    10141 atatttataa gataggaggt ggaaagaatg gattgtattt tttgtaaaat agcgaacgga
    10201 gaaatacctt caactaaagt ttatgaagat gatagagtct tagcttttaa tgacttgaac
    10261 ccagttgctc catatcatat attagttgta cctaaaaaac attatgatag tttaattgat
    10321 attcctgata aagagatgga cattgtgtca catattcatg ttgttattaa taaaatagct
    10381 aaagagaaag gttttgacca aactggattc agagtcataa ataattgtgg aagtgatgga
    10441 gggcaagaag ttaaacattt gcactatcat attttagctg gtaaaaagct tcctaattac
    10501 gaagcgggac aaaactaaac taaattattg aaaaattgag ttccttcttg ataaaaatac
    10561 ttgataaaag taaaaattta gtttataata aacaaagtgt taggttgtaa tgcatattat
    10621 gcaaaaagaa catagttaca gtcccagcat aattgcgcta ttggagggag ggaaaaagaa
    10681 atgtcagaag taagagttag agagaatgaa acattagaca gcgctttaag aagattcaag
    10741 cgtcaatgtg caatgtctgg catcatgtca gaagtgagaa aaagagagca ctatgataag
    10801 ccaagcgtta agcgtaagaa aaaagctgaa gcagcaagaa gaaaaaacgc taagaaatag
    10861 tagtaaataa gaataaagag gtgaagggga tgtcccttaa gcaaaagtta caagaggatt
    10921 tgaaatcttc gatgaaaaac aaagatacag tgagaaaatc tgtagtaact ttaataagag
    10981 cttctataaa gcagtatgag gttgataata gggtcgagct tgatgaagat ggaattatcg
    11041 atgtaatagc aaaacaattg aagcaacgta gagatgcatt agttgaattt gaaaaagctg
    11101 gaagagaaga cttaataaaa gaaacagaag gtgaaattga agttttaaaa gagtacctac
    11161 ctcaacaatt aagcgaagaa gagttagaag aaattgtaaa atctactata tccgaagtag
    11221 gggcaacttc tatgaaggat atgggaaaaa taatgtcagt tatccaacca aaagttaaag
    11281 gaagagctga cggaaaactt ataaataagt tggtaaaaca aaacttacaa tagattgata
    11341 tagcctagag agaaatctct aggcttttta tagtttttgg gaataacgag tcatatttga
    11401 gcatatctat aatcaaagaa aattttgaaa gaaggggaaa aatttgatta tatcagtaaa
    11461 agagattagg aattttttga taaacatatt tataccacta ataataggtt acttaagtag
    11521 catcttaaca atgattatca gtggaacaga tatttcaacc tattatttac aactcacaaa
    11581 gccaagcttt gcaccaccag cttttatctt tcctatagtt tggactatat tgtacatatt
    11641 gatgggtata tcttcatacc taatattaag aaagggttac aacttaccta aggtaagaga
    11701 tgcaattttt tattatggat tacaacttgt attaaacttt atttggagta tactattttt
    11761 tggatttgga cttagattta cagctttgat agatataata ataatgattt tggtttctat
    11821 aataatgatt tcgaagtttt ctaaaataga taagagatct ggatacataa atttgattta
    11881 tttaatgtgg cttgtttatg caggattttt gaactatttt atatggttta ttaataaata
    11941 gaatgtaaag attttgtaaa ttaccctgta cttttaataa tatcataaca aacgaaaaat
    12001 aaatagacta ttaaaaataa tcattttcat atacaataat attaatttaa atacaataaa
    12061 tttataaaaa ttgtatttaa agattaacat attaaagtat tagaattgga aaggcttgaa
    12121 gggagaggtt tttatgcaaa taatagtatc tgggagacaa atgaaattaa ctgacggaat
    12181 aaaaggatac gtagatggaa aattaagtag gttagaaaaa taccttgatc ctgaatcaga
    12241 agtgaaagta acagtaagtg cgaaaaagga tagacaaaaa gtggaggtta caataattcc
    12301 tattaacggt caaataataa gagcagaaga tgtagaagat gacttatatg cagcaattga
    12361 tattgtttgt gacaaattaa gtagacaagt tgtcaaatat aaaactaaag ttaaagacaa
    12421 agttcaaaat aataagagca taagatttga aaatcttgac tttatagata atagcagtga
    12481 atttgatgat tatgattatg atgatgaaga agatgaaaat atagtaatag aaagaagaaa
    12541 gaaatttaat gtaaaaccta tgagttctga ggaggcgata cttcaaatgg aactagtagg
    12601 acacaatttc tatatgttta gaaaccaaga taattttgaa ataaacatag tgtataagcg
    12661 taaagctggt ggatatggtt taatagaaca agattaataa aaattgtata cgtttagatt
    12721 ttatataaga aaaaataaaa aggtaatcat gttgattttt tatcaatatg attacctttt
    12781 tattgtttaa ataatatatt agatattagg tatttattat ataaaaattt taaaactaat
    12841 ctgttacaac atcaataaag tgcataaata cctatttcaa gcataaaatt aattaagggg
    12901 gtaatgctta tgttagaaat atccactgat ttacaagtaa atcaaccagt tattactgtt
    12961 acaagtaata cgtttattag tatagaaaat tatctatcaa tattagaata tgaagttgat
    13021 ttaataagaa taaagactaa agttaaaaca attaaaatat ctggggataa actttcactt
    13081 aagtatatta ctgatagtga gataggtata aaagggatta tctacaatgt tgagtatgtt
    13141 gattaggagg agatacactt gataagtttt ataaggggat actatgttat agttgtggaa
    13201 ggagtaggac tcgaacaatt tttaaatcat cttataagaa atgggataag tgtttacaat
    13261 gtaactagaa taaaaaatac taaaatggag tttcatatag atagacaaga tataaaagaa
    13321 tttaagaatg tttatagagg aagtaagttt gatataaagg taaagcaaaa aactggggtc
    13381 ccttttatta tcaaaagagt ttataagcac aaaggtatgt ggatttgtgc tcttgtttct
    13441 ttatttttat tgatgtctac atcgcagttt gtgacagatg tatatataca atctcctgaa
    13501 ggtataaaaa aagaagctct tagaaatgag ctttataagg ttggagtaag accaggagtc
    13561 tataagaaga gtattgatag aaaagaagtt agagaccata tgatgtcaaa atttaatgat
    13621 gtggcatatt tatctatcaa tgtaaaagga acaaatatat ttgttactgt tacaaaaaag
    13681 gcagaatccc taaaatctac agaccaatca aattattgta atgtaattgc tttaaaaaat
    13741 ggtataatag aaaaggtaat ccctagaagt ggtaaatcag ttattaaatc tggagacata
    13801 gtccaaaagg gagatgtctt attaaatgga gctaatacca agtcaatacc agaagtatgg
    13861 gcaagtactt tttatgagtc cacaaaaaag gcaagttatg tagatactgt aaataaaaaa
    13921 actggagaaa agaaaaatat atatacacta agtttttatg ataaagagtt tacaatgaga
    13981 aaaaatataa aatataaaga ttatgttgtt gaaaataaag aaaaaaaact atcaatagga
    14041 aactatactt ttccaataaa gataaaaacg agtacttttt atgaaacaaa aaaggttgaa
    14101 gtaaaaagga ataaagaaga gttaaaaaaa gaattatctg aaaaagcttt aaaagagtta
    14161 gaatacataa tcccagcttc ggctagaata atagatgtaa aacataactt taaggttaat
    14221 aaaaatatgt tagaatattt aataacagtt caaactagtg aaaatattgc taaaatttat
    14281 cctcttagta agtcagaagc agaaaggttt ataaaggaag aaagtaaacc agatgaaggt
    14341 gaagaggaag ttccttccaa tccagaaaaa agaccattaa atgatataag aaatgagttt
    14401 gatgaggata ataaggataa taaagaccaa aacaattctg atgaaaataa tagtaatcaa
    14461 aatagtaata atagtcaaaa taataatagc aataacaact aataaaatta aacttaaaat
    14521 cttaggagga ctaattcaat tgataataca aaagaaattt acaatagcag atggtaactt
    14581 tgaaagagag ttatttggca actttgatga aaatgtaaaa ttaatagaga agactttaaa
    14641 tatagatgta atacttagag aaggcaatat aattttaatt ggagaagaaa aaaatgtaga
    14701 ttctgcatta aaacttatga atgaattaca ccaaactgta tcaaatggta agcatttaga
    14761 taagcagagt atttcttatt cattatctct tttgcttgaa ggaagtgaac aaaaaataaa
    14821 agaacttgag gggacaatcg tcataactca aaggggaaaa gctgttcaac ctaaaacttt
    14881 aggtcaaaaa gaatatataa aacttataga aaataatgat ataacttttg gtgttggacc
    14941 tgctggaaca ggaaagactt atcttgcggt tgctatggca gtaaaagctt ttaaaagaga
    15001 tgaagttagt agaattatac ttacaagacc agctgtggaa gcaggagaga gtctaggttt
    15061 tttaccagga gatttaaagg acaaagttga cccatattta agaccactat atgatgcctt
    15121 atttgaaatg ttgggtgctg ataaatttaa taagtattta gaaagaggta ctattgaggt
    15181 tgcaccactt gcatttatga gaggtagaac acttgataat tcatttataa tattggatga
    15241 agctcaaaat actacttctg aacaaatgaa aatgttttta actagacttg gatttggctc
    15301 taaagctgta gttacaggag atgtaactca aacagattta ccacaaaata agaagagtgg
    15361 tctaatacaa gctacagaga tccttaaagg tgttccaggt attgggtcta taatgctaac
    15421 agatagagat gttgtaagac atgagttggt acagagaata ataagagctt atgagaagca
    15481 cgacaaaaga gaagaattta aaaaagaaga acgtaaaaaa gtgaaaatgc aggaaaagac
    15541 tttcaaaaga aagtaaaagt gtagtaatat agatatatag cattattaat gaataaaaag
    15601 taggtgtagt gaatggattt gatactagat gatagacaag ataaattaga agtaagtgag
    15661 gagttaattg aaaagataaa ggatataatc atagaatgtt tagattatga aggatatgat
    15721 gataattatg aagtgagtct ttcttttgta gataataaag agattcatga attaaataga
    15781 gaatatagag gagttgatag agtgactgat gttttatcat ttcctctttt aagtgatgat
    15841 tttgaggatg ttgagttaga agaagaatcc ctaggtgaca tagtagtatc tcttgaaaga
    15901 gccttagaac agagtataga gtataaccat agttttgaaa gagaagtatg ttttttaata
    15961 tgtcatagta tgtttcatct tttaggttat gaccacgata cagatgaaaa tacaaaagaa
    16021 atgagagaaa aagaagaaca catattaaat aagttaaaca taacaaggga gtagttttta
    16081 tgaaaccaga aaaaactcgt caaggtataa ttaaagcatt taatgcagct atagaaggga
    16141 tattgtatac atttaaattt gaaagaaata tgaaaataca ttatttaggt tcagttgctg
    16201 ttcttataat aagtttgttt tttaactttt caaaattaga aatgataatg ttacttatgt
    16261 ccatatgctt agttgtagtt gccgaaatgt ttaatacagc tatagaaaaa gctgttgatt
    16321 tagtaactga tgaatatcat gttcttgcta agatagcaaa agatgttgca gctggaggcg
    16381 ttctagtggc agcactaaat tcagtagtag ttggatatat tttgttttat gataaattga
    16441 cagatatatc tggaatatta atttataaaa taagagaatc agaattacat ataaccttga
    16501 tatgtatatt attggttctt atagctgttg tagttgtaaa agcattgact tctacaggta
    16561 ctcctctaaa aggaggaatg ccaagtggtc atgctgcctt agcatttgct atagcaactg
    16621 caattacact tatgactgaa agagttgttg cctctactct tgcatatata atggctgtat
    16681 tagttgccca aagtagaatt gaaggaaaaa tacatacctt ttgggagact attgctggag
    16741 ctttattagg tgtgttgata gctatattag tatttcagct tggaatgttc tataattaaa
    16801 ataaatgtac ataaaatatt taaaataaaa caaatctgtg ctaattaaat ttttatgata
    16861 taatgtagag tattaaataa aaataacttt taattattgt atacaggagg agttgttgtg
    16921 gataacatag aattgttaag gttggcagaa gatgccagac aacattctta tgctccttac
    16981 tcaggtttta gagtaggtgc agctctttta actaagagtg gtaaagttta tactggatgt
    17041 aatatagaat gtgcatcatt aggtggaaca aattgtgcag aaagaacagc tatttttaaa
    17101 gcaatatcag agggtgataa ggatatctat aaaatagcta tagcaagtga taactcagaa
    17161 aataatgagc aaacatatcc atgtggaata tgcaggcagg taattataga gtttggttct
    17221 gatataaaaa taataacggg atatactaaa ggagaggtgt ttgagcatag tataaaggat
    17281 ttgttgccaa actatttctc tggaaatgat ttaaaataaa tttaaaagta taaataaatt
    17341 taagtaatta aaacaagaag ataaaaagtt aggagacagt taatgttcaa atcaggtttt
    17401 gtcagtatag tgggtagacc aaatgtagga aaatctactt taatgaataa tgtagttgga
    17461 gaaaaaatag ctataatgag tgataagcct caaactacaa gaaatacaat acaagcagta
    17521 tatacagatg aagaaatgca gatagtattc ttagatacac ccggtattca caaaccaaaa
    17581 aataaattag gtgaatttat ggtaaaggca gcaacagaag cttttaaaaa tgtagattta
    17641 atattatttg tagttgatga ttctaaaaaa ataggtcctg gagatagaaa gataatagag
    17701 gatttaagaa gtgttaagac accaataatt ttagttgtaa acaaaataga ccaactagac
    17761 caaaaagatg agctatttga tataatcaaa atgtatgata gagaaggtat ttttaaagaa
    17821 atagtaccaa tatcagcatt aaaaggaaag aatacggata ccttaataaa agttatacaa
    17881 aattatttag aagaagggcc aaagtatttt ccagattata tgattacaga ccagccagaa
    17941 agagtattaa tagcagaact tataagagaa aaagttcttc actatcttaa tgatgaaata
    18001 cctcatggtg tagctgttga aattgaaaaa atgaaagcga gaaatgataa ggaaatagta
    18061 gatgtatctg ctgtaatata ctgtgaaaga gattcacata agggaataat aataggaaaa
    18121 aatggtagga aactaaaagg aataggtaag tcagcaagac aagatataga attgctttta
    18181 ggttcacaaa taaatcttca attatgggtt aaagtcaaag aaaattggag aaatttacaa
    18241 aattatataa ataattttgg atataatgat aaataaaggt gggttatatg gataggaaac
    18301 tggatgcttc ccaagattta ttatatgaag taaaatcatt aattgataat aataaagtat
    18361 tggagttaag agaattaata gaggaatatc atataattga tatattcgat ataatggaaa
    18421 atttagagga agacatgaaa attcaattat ttgaagtact tcctttagat atggcatctt
    18481 ctatattaga agaaggtagt gtagagtttt ttattagtat tttatctaaa ctggatgtag
    18541 aacactcgaa aaatatactt gaacttatgt ctcttggaga tatggcagac aagttgagtg
    18601 aacttgaaga agaagaaaga gaacacataa taaatctttt gaatcaagag aatgccgatt
    18661 atgtaaaaga gttactattc tatgatgaag attctgctgg tggaacaatg accactggat
    18721 atatttctat aaataaagat atgacagctc ttgaggctat agaccatatg agagaagaag
    18781 ctgaagaagc tgaaactatt tattatatat atgtggttga tgatgaagaa aaactagttg
    18841 gagtattgtc attaagagaa ttaataatag ctagagacgc aaatatagta gaagatttga
    18901 tgagtgaaaa tataatctct gtttatgtag atgaagatag agaagaagca gtaagattag
    18961 tatctaaata caatttaata gcaattccag tagtagacag acaagaaaaa ttaaaaggaa
    19021 taataacagt agatgatata attgatgtaa tggaagaaga agcaactgaa gatatgtata
    19081 aatttgcagg aagttcagaa catgaaagag aagttgctga aaaagaaaac cctacattga
    19141 gagagcaaat aatatcagca cttagaggac gattaccttg gttaattatt accttggttg
    19201 gagggttgtt ggcttcgtta atactttcta acttagacta tataatgaat cctgtatatg
    19261 ctccattagt attttttata cctgtagtaa ttggtatggg tggaaatatt ggaactcaat
    19321 catcatctgt tacagtaata acattatcta ataaggattt aaattttagc aatgtggtta
    19381 gagaaggtat tgtaggtatt attacaggtc ttttgtgtag tataataaca ggtattgtaa
    19441 tttactttgt tatgagaaag ttagacattg tattaattgt ttcaatatca ttatttataa
    19501 atatggtttt aggtgcaaca ataggtgcgt ttatgcctgt tttattaaaa aaaatggatg
    19561 cagacccatc tacagtttca tcaccaatta tatctacagc tcttgatata actggtatag
    19621 cagtttactt tataataaca acggcattat tgtcaaaaat tgtttaataa ctttatgtat
    19681 gatttatcct cttcttacta aaaatataag tataaatgta agaagggggt tttttaaaaa
    19741 tgaacaatat cagttgggaa gtttttaaaa aaacaggtag tatagatgca tatttatatt
    19801 tctgtgattg caaaaacata catgaagaaa ttgaagaagt aagggagaaa aaagaagacg
    19861 atgataatcc tgaacaccca ggggatagta cttaaagcta taaggtataa ggaaagtgac
    19921 ataatactta cactatttac aagaaagctt ggaaaagtat ctgcaatagc aaaaggagct
    19981 aaaaaaaata agagctcttt gctttcatca tcacaattat tttcttatag caattttacc
    20041 ttaaaaaaac aaggtaatat gtacaaggtt actcaaagtg aaataataaa aagtttttat
    20101 aatatatcct atgatataga ggcattttct tatgctacat atattacaaa attagttgaa
    20161 aattctatac ttgaaaatca gactaataat aggctattta tattacttgc gcagactctg
    20221 tatttgtata cacaggataa tacagacaat aggtttataa cagcagcatt cgagttaaaa
    20281 tttttagact atataggatt taaacctata gtaaataaat gcattaattg tgaatgtaaa
    20341 attttacaaa attctgtgtt taatatctac gaaggtggta tactatgtag taagtgtagt
    20401 aaactttttg ataataatat aaaattagac ttaacaacta taagtcttat ggaatatgta
    20461 ttaagaaatg atattttaac atgtagtaaa gctaaagtat caaaatatat aacacatgaa
    20521 cttgagaata ttctagacaa gtatttgaaa gtttatgttg ataatattaa ttttaaatca
    20581 ttacacgtat tgcaaagtgt aaaaaataat aagggagtgg ataatgatga gtagtaatat
    20641 aactattgaa atggttgatt cagtctttga aagagttcca ggagcaactt atgcagaagc
    20701 aaaagaagca ttagtaatgt gcaatggaga tgtaattgag gcagtaatat atttagaaaa
    20761 taaaaaaaat acttgtaatt gtaaagcaaa atctgctaaa gaaactatgg aagaagtatt
    20821 tggcaaagat ggagaaaata taaagtttca attaaaagag cttcttagaa aaagtagtgt
    20881 agtgagaata atagttgaaa aagatggaaa aactatgatg aacattcctt taacagttgg
    20941 agttgttgga ttagcttttg taccattagc tactttagtt ggattatcag ctgctgttat
    21001 aagtaaatat cgtataaaag ttcaaaatga agaagatgga gaaattgtag atttaggtga
    21061 attgaatgaa gaaaaacttc acgttttgaa gaatatgata actaatgcag caaaagatgt
    21121 gaaggatgta gtagttgaca aaaaagaaga tgacaaagat gtaactgctg accttatgaa
    21181 agaagaatct gaaaagaata aagaagacga agacgaaaat aaatagatat aaagtgactt
    21241 ttaaaaaatg aagagaagat aattataaga attatcacta acattgacaa attttataga
    21301 atttgatatt ctaaaagttg tagaagaact attaaaatag cctttcgtgt agacgagagg
    21361 ctttattgtt ataggaggga tttttatgaa ttttcaagag atgatactgg cgttgcaaaa
    21421 atactggtca aagcaaggtt gtatcatgat gcaaccttat gatattgaaa aaggtgcagg
    21481 aactatgaat cctaatactt ttttaagatc attaggacct gagccttggc aagtatgcta
    21541 tgtagagcct tcaagaagac cagcagatgg aagatatggt gaaaacccaa acagactata
    21601 tcaacatcat caattccaag ttatactaaa accttctcca gataatatac aagaattata
    21661 tttggaaagt ttaaaagaga ttggaattga cccaagtgag catgacataa gatttgttga
    21721 agataactgg gaagctgcta cagttggtgc ttggggactt ggatgggaag tttggctaga
    21781 tggtatggaa ataactcaat ttacgtattt tcaacaagta ggtaatattg aatgtgagct
    21841 tgagacagga gaaattacat atggattaga aagattagct atgtacattc aagaagttga
    21901 tagtgtttat gatttaaaat ggaatgataa aataacttat ggtgaagtat ttaataaagc
    21961 tgagtatgaa aactcaatgt acgcatttga attatgtgat gcagatatgt tgtttaatct
    22021 gtttgatata tatgaaaaag aagcattaag acttatggaa aatggccttg taataccttc
    22081 atatgactat gtgttaaaat gctctcatgc atttaataca ttggacgcaa gaggtgcaat
    22141 aggagttagt caaagagctt catttatagg tagagttaga aatatggcaa agactgttgc
    22201 agaaactttt gtaaaacaaa gagaagaaat gggattccct cttttaaagg acggtgacaa
    22261 ataatgaaaa attatctttt atttgaaatc ggtgttgaag aaatgccatc aagatttgta
    22321 ggcagtacac tagaacaatt aaaaaacaat cttagtaaat tatttgatga aaatagaata
    22381 gcttttgata aaataaatgc gtatgggaca ccaagaagac ttactttagt agtcgaagga
    22441 ttgagtgata gacaaagtga tttagaagag gaaataaagg gtcctgctaa gaaaattgct
    22501 gttgatgcag aaaataattt aacaaaacca gcattaggat ttataaaaag caaaggatta
    22561 agcgaatctg acatatattt taaacaagtt ggaaaagaag aatatatatt tgcgactata
    22621 aagcaagatg gaaaagaaac ttcagaagta ctaaaagaca tattaccaga gactataaaa
    22681 tctgtagtat tcccaaaagc tatgcgttgg ggaggaaaaa atcttaaatt tgcaagacct
    22741 ataagatgga tagttgcatt gttaaatgat aaagtattag agtttgattt agaaggaata
    22801 atatcttcta atgtaacaaa aggtcataga tttttaggtg aaagtacaat agaagttgat
    22861 tctatagaag attattttga aaaattagaa aaaaattata tagttttaga ccaaaacaag
    22921 agaaaagaaa tgataagaaa acaatgtata gaagtagcaa attctttagg tggagaagtt
    22981 gaatttgatg aagatttact tgaagaggta acttatttag ttgaatatcc aactgctttt
    23041 tatggagaat ttgatgtgga ttatataaaa ctaccaaaag aagttgttat aactcctatg
    23101 aaacaacatc aaagatattt tccagtactt aaagaaggaa aactgttacc taactttata
    23161 gctgttagaa atggtgattc ttacagaata gacaatgtta aagctggaaa tgaaaaagtt
    23221 ttagaagcaa gacttgcaga tgctttattt ttctatagag aagatactaa gagaaactta
    23281 gaaagctaca tagaaaaatt aaaaactgtt gtattccaag taaaactagg aactatttac
    23341 gacaaaactt taagacttga aaaattaagt gctgatatac ttgatagttt aggtttagta
    23401 gatgaaaagg cagatactat aagggctgct aaattatcta aggcagattt agttactgga
    23461 atggtaggag aatttgatga attacaaggt tttatgggaa aagaatacgc aaaagttgga
    23521 ggagagaatg atgttgtaag tgaagctatt tttgaacatt atcttccaag atttgcagga
    23581 gatatacttc ctaagactaa tgcaggggtt gccttatcaa tagctgataa gttggattca
    23641 atagcaggtt tctttgctat aggaatacaa ccaacaggtt ctcaagaccc atatgcttta
    23701 agaagacaag ctttaggtat acttagtata cttatggaca gaaaagttgc tgtggatata
    23761 aaagtactta ttgaacatgc tttagataat tattctgaat tagattttaa taaagaagaa
    23821 gttgttgagc aaataatgaa tttcttcaat gagagagtaa aaaatctatt taaagattta
    23881 ggaataagat atgatgttgt tgatgctgtt ttaagttctg atataaatga tgtttctgat
    23941 atgcatatga gagctttaga gcttaataat tggctagaaa aagatgaatt agtggagatg
    24001 cttactgcat ttaatagagt ttctactcta gctcaaaaag cagaaagtga taaaattgat
    24061 gaatctttat taaaagaaga tgctgaaatt agactttaca atgaatttaa acaagtaaag
    24121 gtaaaagtta atgaattatt aaaggataag aaatatggta ttgctttaga tgaatttgca
    24181 tctttaagac cagtagttga taatttattt gatacagtta tggttatgga taatgatgaa
    24241 gctattaaga acaatagact tgctttactt aaacaagtat atgatactat gctagaaatt
    24301 tgtgatttat ctaaaattgt ttataaataa aatttaaact atttataaga aataaggctg
    24361 tctcaaaata gagatagtct tatttttcat tgttgagatg atgaaaatat atttgatttc
    24421 atagaaaaag agtgtctcat aaactaaaaa gttcattttg agacacccat gctttttgta
    24481 agtaaatata ataaattata ttttatactt atcagttatt ttgtttaaaa tattatcatt
    24541 tgaatttacc cattcataaa aattgcctga tgatgcgtcc gatattttat aaagcatttc
    24601 aggaatatca ctagctttaa aatctccaag tgattttccc aatctagtct tacctatttc
    24661 aaaactgcct ccaacaaata attcaaatgc atctgtagat ataccatcaa cattcttctt
    24721 tttaccacat aaacctatgc taccaacttg atgaacacca catgaattta aacatcctga
    24781 tatatataac ttaggtgctt tatttaaaat ttcttcatta ttttgtaatc taaaataatc
    24841 tatgatttca tgtaacattt tctgactatt ttgtatgccc atttgacaaa ttggaacacc
    24901 tatgcaagat atactttgtt ctaattgaga attacaacta atagttttac ttatttctaa
    24961 tattcttttt gcttcatttc catttaaatt taaaatatat aatccctctg tcattcctag
    25021 tctaatcata ggattttcaa cattatctaa ttctttaagt aaagtactta aatcctttgt
    25081 taacaaaata cctccaactg gatgtatata tacagtgtat aaaccatcct gtttttgagg
    25141 tattagtcta gaatcttgta tatctatttt aactccaggt ttagagtaat ctatttcttc
    25201 aatattgagt tcaagactac cattttcttt ttctaattta tagtattctt taaatttttc
    25261 taagaatagt tcttcaccaa gtgattcgac catatatcta accctagctc tgtttttatt
    25321 ttcataattt ccataatcca taaagaattt aatcatacct tcaacacaat ataaggcgtc
    25381 tttcggcttt ataagttcat caagttctaa agcaactttg ggttgtttac ctagacctcc
    25441 acctaagaaa actctgaaat atggttcatc atttttcaaa gtagcaacaa atcctaagtc
    25501 ttgtatacta caatgagcag tatctgtgta acaattagaa taagaaactt ttaattttct
    25561 aggtaaatgg tatgtagtag catttttgat aaaatattta tcagtagcaa ctgcatatgg
    25621 agtaacatca aagacttcac tagggtcaac tccagataat ggagataatc cgacatttct
    25681 aggataatta ccaccaccac ctctagtgaa tatatcattt tttattcctt cttccatgat
    25741 atcaacaatt gaatttatag ataaatcgtg taattgtata gcttgtctag ttgttaagtg
    25801 tatcttatct aagttgtgtt taatagctat ttcatagatt ttattaagtt gatactgaga
    25861 taaaactcca gcagatactc taagtcttat cataaaagat tttttgtctc tttgagcata
    25921 aacaccatat ccaccagaaa atcccttata ttgcatttta tttatttctc catttaaaaa
    25981 ttttgaagaa tattctttaa aattaggtag ttcatttaac aatatctttt taaaattatc
    26041 cattatttca cctcattgtt agtcttgtat aaaaatatat ctattgtata ata
//
LOCUS       DAMJVX010000037        26019 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMJVX010000037 DAMJVX010000000
VERSION     DAMJVX010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26019
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.039"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            213..3761
                     /locus_tag="PC407_001450"
     CDS             213..3761
                     /locus_tag="PC407_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2496902.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINNEMVIALMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTISCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKIIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDNFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADMALY
                     SAKGKGKNCYTLYDKKIDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESASNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMRNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEVTSIEAEENT"
     gene            3946..5880
                     /locus_tag="PC407_001451"
     CDS             3946..5880
                     /locus_tag="PC407_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDF2496903.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLENAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5986..6981
                     /locus_tag="PC407_001452"
     CDS             5986..6981
                     /locus_tag="PC407_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2496904.1"
                     /translation="MKKYISIILLVVVTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSNIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6953..7738
                     /locus_tag="PC407_001453"
     CDS             6953..7738
                     /locus_tag="PC407_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2496905.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7726..8424
                     /locus_tag="PC407_001454"
     CDS             7726..8424
                     /locus_tag="PC407_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2496906.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8547..9020
                     /gene="ybaK"
                     /locus_tag="PC407_001455"
     CDS             8547..9020
                     /gene="ybaK"
                     /locus_tag="PC407_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDF2496907.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9217..10056
                     /locus_tag="PC407_001456"
     CDS             9217..10056
                     /locus_tag="PC407_001456"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HDF2496908.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10069..10617
                     /locus_tag="PC407_001457"
     CDS             10069..10617
                     /locus_tag="PC407_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HDF2496909.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10683..11864
                     /locus_tag="PC407_001458"
     CDS             10683..11864
                     /locus_tag="PC407_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HDF2496910.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYIYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12037..12300
                     /locus_tag="PC407_001459"
     CDS             12037..12300
                     /locus_tag="PC407_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2496911.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            12826..13509
                     /locus_tag="PC407_001460"
     CDS             12826..13509
                     /locus_tag="PC407_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2496912.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFSIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGEFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            13520..14761
                     /locus_tag="PC407_001461"
     CDS             13520..14761
                     /locus_tag="PC407_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2496913.1"
                     /translation="MKIFSNKDIKIFFLILISILVMFIIVGQIVVISITNNYKKTLIE
                     HDYNIAGYLYSIGVDKSKIPSAFVAHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLF
                     QSKYIKTMSIYLLVFFTMIIFILYMYFFRQQSKIEEASYKIDLFMNGNINERLNTYEE
                     GSLSKLFSSIDVMSTSLNTHILKEKQSREFLKNTISDISHQLKTPLTALIMYNQILQE
                     ESHNSDVVEAFVQKSQSELERIETLIKNLLKITKIDSKTIVLNKNNVNLKNLIVKILY
                     SFETRAEKEGKVISINGSENTLLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFET
                     TITTIVSVKDTGNGIHVEDIHHIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTIS
                     VESKLSQGSTFTISFLKLTNM"
     gene            14833..15516
                     /locus_tag="PC407_001462"
     CDS             14833..15516
                     /locus_tag="PC407_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2496914.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGRIFIDGKEICSMKEEKQTIFRRRNIGFIFQAYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKNHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSNDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGV
                     LTDLGVNRE"
     gene            15513..17843
                     /locus_tag="PC407_001463"
     CDS             15513..17843
                     /locus_tag="PC407_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2496915.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIIIAVCLVTAIFGLADMAI
                     QCQKIQFIKDDGNWHIVIRNIDEKFISEINSRVDIAHHGRGQVVDSGEFKSKEILIQG
                     LDEEVAKQFNLEIIRGRYPIGENETLIDEDAVKSFNLKIGDFIKIKLADGRNKNYKIV
                     GEYSSISSLRATDKHGLILSILGTQAIKKSETYDYYYITFKEKVNIRKATSDIKNTFN
                     LSDKQIGKNERLLSIIGQGDSDFSKPLYLTAGLLFVLVLVASIIMIYNSFNMSVIERV
                     KFFGLLRCLGSSKHQVKKFVVLESLILSIKAIPIGLVLGSLVTIISSIFLKYVNELFS
                     SMPILKVSFIGIAFGIIVGFLTVILSAIIPAKKASRVSPLSAVRGNLMSKEILNNGKT
                     SKVANTGSFKIETSMGLNHAFSRKKSFVLMTSSFAISIILFLGFSVLIDLMYQGLKPM
                     KPEAPHISIVDPNFKSVLTKEDINEISNIKGVKKVYSRMMTDIEATYNGKKTKSQLIS
                     YEKYQFNWAKKYLLKGEVNENKLQSENLALVEEGHGFDIGDKIYIKNRNAEKELEVGG
                     ILSSIPFEAKDKYIGNIITSENTFTNITGIKGYIIMDIQVDKDTPGDLIENIRRVVDD
                     NLEVRDKRQSNLEVKNSFYTMATFVYGFVFIIAIISMFNIINSMNISVTSRKNYYGVM
                     RAVGISNRQLRRMVIVEAGSYAMSGCLLGSILGLMLHRYMFISLVSSMFHLEWQVPFN
                     LLLITVLTMIIITLLSVRKPVKKICELDIIDSINAQ"
     gene            complement(18024..18866)
                     /locus_tag="PC407_001464"
     CDS             complement(18024..18866)
                     /locus_tag="PC407_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2496916.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKAGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            19161..19895
                     /locus_tag="PC407_001465"
     CDS             19161..19895
                     /locus_tag="PC407_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2496917.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            19905..21038
                     /gene="nagA"
                     /locus_tag="PC407_001466"
     CDS             19905..21038
                     /gene="nagA"
                     /locus_tag="PC407_001466"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2496918.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNITHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            21137..21886
                     /gene="nagB"
                     /locus_tag="PC407_001467"
     CDS             21137..21886
                     /gene="nagB"
                     /locus_tag="PC407_001467"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDF2496919.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKLENTHLP
                     NGMADDIEKECLNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            22061..22333
                     /locus_tag="PC407_001468"
     CDS             22061..22333
                     /locus_tag="PC407_001468"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496920.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPIGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            22993..23697
                     /locus_tag="PC407_001469"
     CDS             22993..23697
                     /locus_tag="PC407_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2496921.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            23699..25186
                     /locus_tag="PC407_001470"
     CDS             23699..25186
                     /locus_tag="PC407_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2496922.1"
                     /translation="MNNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVFSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            25391..>26019
                     /locus_tag="PC407_001471"
     CDS             25391..>26019
                     /locus_tag="PC407_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2496923.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFY"
BASE COUNT        10378 a         2678 c         4526 g         8437 t
ORIGIN      
        1 caaaaataaa gttatagttt ttaatattaa ttaagaatga tgaaatatga ataaagattt
       61 tacaaataaa tcttaagcta tcttatttaa ttctaatgca ggaagaaggt aaatgtatat
      121 tgaaaaaatc atagaaatag aagtaaacaa cttaaacaaa tacaaaggtt tgcaaaagaa
      181 gtaatataat tatatacaat gaggatgtga gtatgatgaa cagactaaag ccaagaattt
      241 taggtattag tatcttgttt attattataa ttattttaac atgtggagct gttttcaagt
      301 ttaaatttaa attagataat aaattaaaag aaaccacaca tacatatttg tctgagttta
      361 cagaacacaa tataattaat ctcaagacaa gacttagtgg tcaatttgac atgcttgaat
      421 ctattgctaa cttcatagga aacttgaata atataaataa tgaaatggtg attgctttaa
      481 tggattctga tgtaaagcga agttctttaa ttagaatgac tatctctaca ttagatgggg
      541 aatgttattc taatgatgga atcacccata atgttcaaga tagagattat tttaaaaaag
      601 ctaaaaatgg agaaaagaat atttcagagc ctttaaaatc tattgttaat aataaagaaa
      661 taatagtttt atctacccca gtttataaaa gtgggaaaat agttggtgtt ttaagtggaa
      721 tttatgattc aaaaaagtta aatgagttat taggattatc tgcatttgat gaaaaagcag
      781 atgtttttgt tgcaaagaaa aatggagata tagttgctca aaaaacaaat aaaaatagaa
      841 agacgatatc atgtatattt cagctttttg acaaagataa ttgtgatgaa gatgattgtc
      901 taaaaactat aaaagaaaat atgaaaaatg ataagtctgg atattttaat tataacttag
      961 ataatgaaaa atttatgtta aactatcagc cacttggagt taatgattgg tatgtttttt
     1021 ctgttgtgcc aaataaaata gtgtctattc agtcagagaa tatatcttat gatgtatata
     1081 tttttgcatt agaaatacta ctagttatat tgatttttat aatatatata gcatgtatta
     1141 taaataataa caccaaaatt attataaatg aaaaagaaaa atttaaagta ctcactgata
     1201 atatattagg aggggttaaa acctgtttaa aagatgaata tatgactatg acatatgtaa
     1261 gtcaggggtt tttagagctt acaggttact cttgtgaaga tttaaataaa atatttaaaa
     1321 atagatttaa agacatgatt tataaagatg atttagacaa gtttaattgt attattctaa
     1381 atcaattaga aaaacaagat aaaattgaag ttgaatatag aattgtaaga aaagatggaa
     1441 gtataatttg gattttggag agaagtaagt ctataaatga taataaagga agtgagtgta
     1501 ttcagggagt tttaactgat ataacagaac ttaagaaaat acaagaagag ttagattcaa
     1561 aaagatttga aatacaggct atcaatgata gtattgttgg tggaatagtg ataactgaga
     1621 taaatgatga ttttgatagt atatatttaa atgaaggtta tttaaatatg attggatata
     1681 cacgcaaaca actggaagat gagctaaata ataaattaat caaacttatt tttaaagatg
     1741 atagaacaga attaattaat agtataaaca aacaactcaa attaagtgat acatttgcat
     1801 ctgaacaaag agttgaaaaa agggatggta ctattatatg gattttatta aaaggaagaa
     1861 agataattta tgataatgga aaatctgtac tttgttgtgt tattgtagat atcacttctg
     1921 ctaaattatt agagcaagaa cttaaaaaga gtgaagaacg tcatgaaatc attctaaagc
     1981 agacgcaaaa tatcataata gaatgggata tagaaaaaga taaaatgaat atatcaccta
     2041 attggtataa taagtttaaa tatgacccta taactgaaaa cgcaacaaaa aatataatta
     2101 aatcagtaga tatatatcat gatgatatgg ataagtttat ttcattaatt aaacttactt
     2161 cacaaggtag tgagtatgaa gagtgtgaaa ttagacttag aactagagat actatttacg
     2221 cttggtttag tataaaggca acaactttat ttgataataa tggtaaacct tataaagtag
     2281 taggaagttt gactgatatt gatgaatcaa aacgagaagc tgaaaagttg aaagaaaggg
     2341 cagaaaaaga ccctcttaca gatttgtata ataaaaaagc atcacaatca cttatagaag
     2401 agtacataac attggataaa aaaagaaaag gtgctttaat gattattgat attgataact
     2461 ttaaaggaat aaatgataat ttaggacatt tatatgggga tgctgtatta agtgaaattg
     2521 caaatgacct aataaatcta tttagagcaa ccgatatagt aggcagaatt ggtggagatg
     2581 agtttattgt atttataaaa gatatttctg aaacaagaga tattataaat aaagcagaag
     2641 aattggtaaa aacatttgaa aggtcttttt taggtaaagg acgaaattat aaaatatcat
     2701 taagtgtagg aattgctaag tttccacaag atggtatgga ctatatgaca ctatttagaa
     2761 atgcggatat ggctctttac tcagccaaag ggaagggtaa gaattgttat acattgtatg
     2821 ataaaaaaat tgataaagtc caatatataa ctaatacaag aaatgcagga gatagtgata
     2881 atgtagagat gtcttttaaa gaacatatca caaattatat atttgatata ctatatgaat
     2941 caagtgatat agaagtggca gtaaatttaa ttctaagtat tgtaggtaaa cactttaatg
     3001 tgagtcgcat gtatatattt gaaaattcag atgataatcg agtatgtaaa aatacatttg
     3061 aatggtgtaa cgaaggtgta acctcggaaa ttaataatct taaaaatatt tgttacaagg
     3121 atttaggcaa ctatctttta aattttaatg cagatgggtt attttattgt aatgatattg
     3181 aaaatttagg tagtgatgtg aaaaaagtac ttaagccaca aggcatacgt tcaatattac
     3241 aatgtaaaat tttagagggc aatgaattta gagggttcat aggatttgat gaatgttcat
     3301 ataatcgtat gtggactaat gatgaagtga acactttgac atttatatct aaaacgctca
     3361 gtatgttttt attaaaaatg cgtattcaga gtaagctaga agagtcttta acaattcaaa
     3421 aatctgtgat ggataatatg gatatatggg catatgttgt tgacaggaat acctatgaat
     3481 tattatttat caataaaaaa atgcttgagt cagcatcaaa cactcatgta ggtgatttat
     3541 gttataaagc gatatggaat aatcaagata aaccttgtaa aaaatgccct atgatatatt
     3601 taaaagatgg tgatgatact tataaatcat ttatgcgcaa taaaaagttt gaggtacagg
     3661 caaatataac tacaactact ttgaaatggt ttgatgggaa agatgcatgt ttaatatttg
     3721 gaagtgaagt aactagtatt gaagctgaag aaaatactta aaatactgtg ttatatatat
     3781 acttaaaatc gcaatttgaa tttattagac ttaaaatgtc tatataaatc atttaagttg
     3841 tggttttaag tattttttat gatataatta agcgttaaga acaaaattaa aagatacttt
     3901 tgtataaaaa aatatttgtt aaatatatat tgagcaggtg ataatatgga aaataagttt
     3961 ttaccaataa gcaaacaaga tatgatagat agaggatggg aagaattaga ttttgttcta
     4021 gttacaggag atgcttatgt ggaccatcat agttttggaa cagcaataat atctagagtt
     4081 ttagaaaatg ctgggtataa agttggaata atagctcaac cagactggag aactacagat
     4141 gattttatga agctaggaaa accgagactt gcatttctag ttaatgcagg aaatatggat
     4201 tcaatggtca atcattattc tgtaagtaaa aagcatagag aaaaggatat gtattctcca
     4261 ggtgggaaaa tgggttatag accagataga gctactatag tatattgcaa taaaataaga
     4321 gaagcataca gtgatgttgc aatagtaata ggtggaattg aagcgagttt aagaagattt
     4381 gcacactatg attattggag tgataaggtt agaaagtcta tgttaataga tagtggagca
     4441 gatttattag tttatggtat gagtgaaaag cagattgtag aagtagccaa tgcacttaat
     4501 gatggatatg acccaaaata tattagacat attgatggga catgctatat ttcagataca
     4561 ttggaagaaa tatatgataa atatatatta attccatcat ataaagaaat ttgtgaagat
     4621 aaaatgaagt atgtagaagc ttttaaaatt caatatgatg agcaagaccc atttagaggt
     4681 aatataattg ttcaacctca tggaagtaag tatctggttc aaaataagcc agaaaaacca
     4741 cttagtagag aagaactaga tgaagtatat ggattaccat atcagaagac ataccatcct
     4801 gtgtatgaaa aatttggagg aataccagct atagaagaag taaaatttag tatagtaagt
     4861 tcaagaggct gttttggtag ttgttcattt tgtgcaataa catttcatca aggtagagca
     4921 gtgcaaagta gaagtgaaaa atcaattatt gatgaagcaa taggaataac aaatttagat
     4981 gattttaaag ggtatataca tgatgttgga ggtccaacag ctaattttag aagaccagca
     5041 tgtaaaaaac aaattactaa aggtgcttgt aaaaatagac aatgcttatc tccatcacca
     5101 tgtaaaaatt tagatgcaga ccacagtgaa tacttacatt tacttagagc tgtaagaaaa
     5161 ttacctaaaa taaaaaaggt gtttgtgcgt tctggaatta gatacgatta tgtaatggca
     5221 gataagaaca ataaatttct tagagagttg atagagcatc atgtaagtgg tcagttaaag
     5281 gtagccccag aacatatatc agaagaagtt ttaaagtata tgcaaaaacc agcaggaaaa
     5341 acttatgata aatttagaca gaagtttttt gctataaatg aagaattagg taaaaagcaa
     5401 tatttgatac cctatttaat gtctagtcac cctggttcta ccttaaatag cgctatagag
     5461 ttagcagagt atttaagaga tactcactac caaccagagc aggttcagga tttttatcca
     5521 actccaggga ctctatctac aaccatgttt tatacaggta ttgacccgct tacaatgaag
     5581 ccagtttatg ttcctaaatc aaaacgtgac aaggcaatgc aaagggcttt attacaatat
     5641 agagcaccta gaaattatga tttggtatac tctgcgttag ttgaagcagg aagagaagac
     5701 ttaattggtt ttggacatag atgtcttata aagccaaaaa atgaaaagcc ttattttaat
     5761 agaaataatt ctaaaaaaaa tgtatctaaa ggaacaaata aaaacaaaaa aactaataca
     5821 aataacagaa gtaataaaaa tcaacaaaaa agttcaacta aaaaaaagaa aaaacattaa
     5881 agtataaata aaaatactta aaaaagaagg attattcgat aaattaacag aaattagtat
     5941 aaataaatat aattatttta atgacgttac ctttgggagg aagagatgaa aaagtatatt
     6001 tctataatat tattagtggt ggtgactatg gttgttttgg taggatgttc accaggaaaa
     6061 gataacccta aaaataagga gctatcagtt gtcaaacaag taaaagtagc tgttccagat
     6121 ggactaccag cagtggcaat agctaaactt gcaaatgaaa atccagagat taaagaagga
     6181 tatgaaacag tgtatagcat tgaaaagaca cctgaggcga tttccactag agtgatgaaa
     6241 aaagatgcag atatagctat agttccttca aacatggctg caatagcgta taataaaact
     6301 tcatcatata atattgttgg aactgtagga atgggttcac tttatttggt gtcaacagaa
     6361 aatataaaag attattctga tttagttggt aaagaagttg gatgtactgg aaaaggactt
     6421 actccagaca taactataaa aagtcttttg ttacaaaagg atataaatta ttcaaatata
     6481 aaatttaatt atgtaaattc agcaagtgaa ctggtaccat tattggctac tggtaaagtt
     6541 aagacaggta ttgttccaga acctgcactt agtgctttga tgtctaaaaa tccagacata
     6601 aaaattataa aaagtttaaa tgactcatgg aaggaagtaa gtggttcaaa agacggttat
     6661 cctcaatcta ctttaattgt aaagtctgaa tttttaagag ataataaaga ttttgttgat
     6721 tcttttgtag gtcaattatc aaatagtata gattgggcaa acaaaaatcc tgaagaatta
     6781 ggtgcatatt ctgaaaaaat aaaaatatct actgaatcaa agataatagg aaaatctctt
     6841 gaaagagcta atttaaaata tattccagtt aaggacatga ttaaagatta taaaaattac
     6901 tatgagaaat tagctaattt tgatgataag acattaggag gcaaagtacc agatgaagca
     6961 atatactttg ttgagaaata aaaaaaataa agaacaattt atagataaaa tggaggtaac
     7021 actttcttgt gttatcctct tatttttatg gcaaattatt gctttaaaaa ttgataatga
     7081 tattttttta ccaacagtag gtcaagtatt taatagtata aaagagatta tgctaaattc
     7141 tagtttctat atagatatac tttactctat ggggagatgt ttttttagtt ttttactggc
     7201 tatgattttt gccattatac tttcaatagt atcttattta aatagatttt ttagaaactt
     7261 attaaagcca atcaatgctt tgacacgttc aataccaact atgatattgg tagtacttgc
     7321 acttatttgg tttgaaaagg atagtacccc ttttatagtt ggatttacta tagtttttcc
     7381 tatattgtat gataacgttt tgggcgctat attgaatata gacaagaact tattagaaat
     7441 ggctaatgtg tataaaataa ggtttataga taaggtgtta aaaatttatt taccagccat
     7501 aaaatttcaa attatatcta tactagtatc aacattctct ttaagtttga aagttgctat
     7561 agcaggagag gtatatagtc aacctacata tggtattgga actatgatac agactgaaaa
     7621 aattaacttt aatacatctg gaatatttgc atggataatt attgttcttt taatatctgc
     7681 tattttacaa atagcccaaa agtttttagc aaggagagct tttttatgga aaagataaag
     7741 ttgagtattg aaaatataaa taaaagatat gatagtcgta ttatatttag agattttaat
     7801 attgattttt atgtaaatga ggtaaattgt atccttggaa aatctggttg tggaaaaact
     7861 acactcttga acataataag tgggattatt aaaaatgaca ccaacaatct aaatatcaaa
     7921 gaaaatttaa atagagtagg aaataagctt gaagctagtt acatatttca agatgataga
     7981 cttatagact ggttaacagt agaagaaaat ataaagatag ttgtaaataa atactataat
     8041 aaaactcaat taaataaaat ttgtgatgaa tacttggaat tggttggaat tagtgattat
     8101 aaaaaattct atcctcagat gcttagtgga ggtattagac aaagagtaaa tattgctaga
     8161 gcttttatat atccatcaaa aaacattata atggatgaac cttttaaatc tattgatgca
     8221 aagaatacac agcttataat ggataatttc agaaatatat taagaaaaga aaagagaact
     8281 gtattatttg taactcataa tattgaggaa gcattgtttt tagcagatag gatatttatt
     8341 ttaggagact ctccaattag aataaaaaaa atattaaaaa attctaagga attggaaaaa
     8401 aatgaagttt tgaagttgat atagagttat aataatactg tcgttttaaa atatatttgg
     8461 ttaaaatcta taagactata ttaaaatagt acagaatatt caaaactgtg tataatgttc
     8521 gtattttata aaaaggaagt gaatgtatgg ttaagactaa tgctatgaga atattagatt
     8581 caaataagat tgattataaa gtaatgtctt atgaagtgaa aagtgaacat gtggatggtg
     8641 ttgaggtggc acatgatata ggaagagatg taaatgaagt gtataagaca ttagtaactc
     8701 aaggagttag taagaatata tatgtatatg taataccagt acatgaaaac ttagacttaa
     8761 aaaaggctgc aaaagtagct aaagagaaaa gtgtagagat gattcatgta aaagatataa
     8821 ataagcttac aggttatatt agaggcggat gttctcctat aggtatgaaa aagctttata
     8881 aaacttttgt aaatgaaagt gcaaagaatt tagatacaat tattgtgagt gcaggaaaaa
     8941 taggatatca aatagaacta tctccatttg atttacaaag acttataaaa gtagaatttg
     9001 tagatgtaat aaaaaaataa gaattacata taaaatatag agcttatatt cttaataata
     9061 ggtgttgtaa tattaataaa atgaataata attttaaaat aaatattcag ttacttatgt
     9121 gtaattggta taaaaatatt gagaaatttt aaaaacaatg gtaaagtgta agtataatca
     9181 cgaatatttt ttataaagta atggagggat gttcgcttga gaaaaataaa atctgaacaa
     9241 atcgtcgagc aggtaaaaaa actttgtata gaagcgagcc tatatttagg agaagatgtt
     9301 ttaagttgta taaaagaaaa agcgaaaagc gaaaagagtg aagttggaaa aaatatattg
     9361 aatatattag ttgaaaatgc tgaaatagct aaagaaaaaa atataccaat atgccaagat
     9421 actggtatgg ctgtattttt tgttgaaata ggacaagaag ttttaattga aggagacaca
     9481 cttactgatg ctataaatga gggagtaaga caagggtatg aagaaggcta cttaagaaaa
     9541 tctgttgtaa gtccaattaa cagagtaaat acaaaagata atacacctgc tgttattcat
     9601 tatgatatgg taaaaggtga taaaattaaa atagaatttg cagctaaggg atttggaagc
     9661 gaaaatatga gtaagatgaa aatgttaaag ccatcagatg gattggaagg aattaaaaaa
     9721 ttcataatag atacagtttc agaagctgga cctaacccat gccctcctat ggtaattgga
     9781 gttggtatag gtgggactgt ggacaaatgt gctcaaatag ctaaaaaggc actttttaga
     9841 gaactaggtg aatttaataa ggacgaaaat atagcaaaac ttgaaagtga actattaaca
     9901 gctattaata aacttggtat aggacctcaa ggcttaggtg gtactacaac agctttggga
     9961 ttaaacatag agacttttcc tacacacata gcaggtctac ctgttgttgt aaatataaac
    10021 tgccatgcat caagacataa gaaggtagta atttaggagg aaaataaaat gataaaaata
    10081 actacacctg taaatgaaat tgatatagca aaattaaatt gtggagatac aattagtttg
    10141 agtggtatac tttatacagc aagagatgct gctcataaaa gattgataga ttgtataaat
    10201 aaaggtgaag aattaccatt tgacgtatat ggtcaaggaa tatattatgt aggacctact
    10261 cctacaaagc caggagaagt tataggtgct gcaggtccaa cgacgagtta tagaatggat
    10321 gatttgacta tacctctttt agaaagaggt cttagattga tgataggcaa gggaaaaaga
    10381 agtgatgaag ttatagaagg tatgcaaaaa tacggagcag tttatcttgc tgcaatagga
    10441 ggagctggag cgtatatttc aaattcaata aaatcatgtg aaattattgc atatgaagat
    10501 ttaggagctg aagctataag aaagattgta gtagaagacc ttaaacttac agtagcaata
    10561 gatagctatg gaaataatat atatgagcaa ggtcgagcaa tttatgaatg taaataatgt
    10621 ttagaaataa atatgaatat caaaattaaa aatagttgtg tatattttaa ggaggaaaat
    10681 tgatgtctaa ggattatgca aaattggcat tagaaatgca tgaaatcaac aaagggaaag
    10741 taagtgtaga aagcaaggta gaagttaaaa ctaaggatga tttatcaaca gcatatacac
    10801 caggggttgc tgaaccttgt ttaaaaatac atgaaaatca agatgatgtt tatatataca
    10861 cttctaaagg gaatctagta gcagtagtta gtgatggaag tgctgtactt ggattaggta
    10921 acattggtgc tgaagcatct atacctgtta tggaaggtaa agctatatta tttaaacaat
    10981 ttgcagatgt agacgctttt ccaatatgtt taaagactaa tgatgtagat gagatagtta
    11041 aatctgtaga gttgatggag cctgtatttg gaggtataaa tttggaagac atatcttctc
    11101 caagatgttt tgagatagaa gagagattaa aaaaatcttt aagtatacct gtttttcatg
    11161 atgaccaaca tgggacagca ataatagttg cagcagccat aataaactca ataaagttaa
    11221 ttgaaaataa aaaaatagaa gatttagaaa tagttataaa cggagcagga gctgctggta
    11281 tagccatagc aaagatacta cttaatatga atgttaaaaa tataatctta tgtgatagaa
    11341 gtggagcatt ggaagctagt atagaaaatc taaactatgt tcaaaaagaa atgttaaaag
    11401 taacaaatat aagaaatgaa aaaggacctt taaaagatat tataaaagga aaagatgtat
    11461 ttattggtgt ttcaggtcca ggagctgtta caaaggaaat ggttgaaagt atgtctgaga
    11521 agccaataat attggcaatg gcaaatccta ctccagagat aatgccagaa gaagcaaaat
    11581 taggtggagc atttataatg ggaacaggtc gttcagactt ccctaatcaa gtcaataatg
    11641 tactggcttt tcctggaata tttagaggag cattagatgt taaggctaaa gagataaatg
    11701 aagaaatgaa gatagctgct gcatatgcaa ttgctaatac aataagtgat gaagaaatat
    11761 gcccagagta tatattacca gatgtattta ataagaatgt agtaaaaaat gttgctaagg
    11821 cagttaaaga agcagcgata aaaactaaag taaatagaat ataaattttt ataacttatt
    11881 aaaaactaga aaagtataaa agactagtga aattatgtac agtgggatga ttttattagt
    11941 ctttttattt taaatatgac acaataatgt catattaagc ttgatataat tttaatatgg
    12001 ttaggagcca aagaaaaata taaggagtga tttaaaatgg aagaaactaa attttgccaa
    12061 tgctgtgcaa tgccaatggg acaaactgat gaattatatg ggacaaataa agatggaagt
    12121 aaaagtactg attattgtag ttattgtttt aaagatggtg agtttacagc tgatataagt
    12181 atggaagaga tgatagaagt ttgtatccct catatgcttc aaagtaataa ggatatgact
    12241 gaagatgaag caagaacaat gatgaacaat ttctttccaa cattaaaacg ctggaaataa
    12301 ttttatgcaa aattagttat atgcaaaaca tgaaatatgg tgttgagaat aaggggtggt
    12361 gtgctttata gcataccacc tgttttgtta cttgctagtt cagttattct tgtatgctta
    12421 aatatgttct catctctctt tctattttaa attgtaaaaa tctaaaatat gttatttata
    12481 ttctacttct ttatccatgt ctttttctac ctcaagtcaa atacaactac aaaatcatca
    12541 ttttcaattc actgtttaat agaattaaca gataaagagt tataaaattt tctatttttt
    12601 atataaaata atacaggtca agctgacttc tagctacttg ggatagtgta tatatttatg
    12661 atagttatag gagtaatacc aatcattaga ctgttgattt gtgtagtctt ttgtaaaaac
    12721 atttgtttta atatattgag tttttgaaaa agtaaaatta taattataat aaattaagtg
    12781 taataatgta ttagaagtaa aaataattaa aatagaggtg attaaatgag cagtatattg
    12841 ttagttgaag atgatttaag cttgatatat ggattagagt tctctataca aaaaagtgga
    12901 tttagtatag acatagcaag gacagtaaaa gaagctatgc aaatgtatga ggaaaaaaat
    12961 tatgacttat tgcttttaga tgtatcccta cctgatggta atggatttca gatttgcgaa
    13021 aaagtacgag aggtttctaa tatacccatt atatttttga ctgcttcaga tgaagaggta
    13081 aatgtagtga tgggtcttga tatgggtggc gacgattata taacaaaacc ttttaaatta
    13141 aatgaattaa tttcaagaat aaaagcatta cttagacgtt ataatgttgc aagtaatgtg
    13201 aatgaattaa gttcaaataa tattactatt aaattacttg aaaatcgtgt ttttaaaggt
    13261 gaatttgagg tggaacttac tacagcagaa tataaattat tgtgcttatt tatgaaaaat
    13321 aaaaatattg tacttactag aaaaaacata ttggacaagt tatgggatgg aaatggtagc
    13381 tttatagatg acaatacatt atcagtatat gtaagacgat tgagaaataa aattgaagat
    13441 aatcctgaaa atcctaagtt tctattgacc gttagaagaa tgggttataa gtggaatgta
    13501 attaagtgag ggtattaata tgaagatatt ttctaataaa gatataaaaa tatttttttt
    13561 aattcttata tctattttag taatgtttat tatagtaggg caaatagtgg ttataagtat
    13621 aactaacaat tataaaaaaa cgttgataga acatgattat aatatagctg gatacttata
    13681 tagtataggt gttgataaat ctaaaatacc atcagcattt gttgcacatg aagataaata
    13741 tattttaaaa ggaaaagaaa tactagatac agctggatat ggatttgata tagatgttag
    13801 ctttacacca agtgtaaaat tgtttcaaag taaatatata aaaactatgt ctatctattt
    13861 attagtattt tttactatga ttattttcat actatatatg tattttttta ggcaacaaag
    13921 taagatagaa gaggctagtt ataaaattga tttatttatg aatggaaata taaatgaaag
    13981 actaaataca tatgaagaag gtagcttatc aaaattattt agttcaatag atgttatgtc
    14041 gacatcttta aatacacata tattaaaaga aaaacagagc agagaatttc ttaaaaatac
    14101 aatttcggat atatcgcatc aattaaaaac tccacttaca gctttaataa tgtacaatca
    14161 gatactacaa gaagaaagtc ataatagtga tgtcgttgaa gcttttgtac aaaaaagtca
    14221 aagtgaacta gaacgcattg aaacattgat taagaattta ctaaagatta caaagataga
    14281 ttcaaaaaca atagttctca ataaaaataa tgttaatctt aaaaatttaa ttgtcaaaat
    14341 tttgtatagc tttgaaacta gggcagaaaa agaaggaaag gttatttcta taaatggttc
    14401 agaaaataca ttgttgttct gcgattatga atggatgtca gaggctttaa gtaatcttat
    14461 aaagaatgca ttagataata caaaagagaa tgataaaata acgatagagt ggtttgagac
    14521 gactataacc accattgttt cagttaaaga cactggaaat ggaatccatg ttgaggatat
    14581 tcatcatata tttaaaaggt tttatagaag taagttttca aaagataatc aaggtttagg
    14641 actgggatta ccactagtta aatctattgt ggaacaacat aatggtacta tttctgtaga
    14701 aagtaaacta tcacaaggaa gtacattcac cataagtttt ttaaagctta ctaatatgta
    14761 agataaaata catcttgcag taaggtatat agattattat tatattaatc aattagaaag
    14821 gtgagagaga aaatggatat tcttgtggtt aataatttat gtaaaacata tggaaatgga
    14881 tatacaaaag tagaggcatt gaaagatgta tcctttacag tttcaaaagg agagtttgtg
    14941 actgtagttg gagaatctgg ttcaggaaag agtacactat taaatctctt aggaggtctt
    15001 gatactccaa catcagggag aatatttatt gatggtaaag aaatatgttc aatgaaagag
    15061 gaaaaacaga ctatttttag acgtcgtaac atagggttta tatttcaggc atataacctt
    15121 ataccagaac taactgtgga gcaaaatata atattcccat tgttattaga ttataaaaaa
    15181 ccagatacta tttacttaga tgaagtattg actattttag gtcttaaaga taggaaaaat
    15241 catcttccaa atcaattatc tggtggtcaa caacagagag ttgcaattgg aagggctttg
    15301 attacaaagc caagtattat actggcagat gagccaacgg gaaatcttga cagtaaaaat
    15361 agtaatgatg taatttcact tttaaaagtg actgcagaaa agtataatca aaccattatt
    15421 atgataactc ataatacgaa tatagcttct acagcagata gaatactaca ggtttctgat
    15481 ggtgtgctta cagacctggg ggtgaataga gaatgagaag ctacttagaa ttagtaccac
    15541 aatatgcaaa agtacataag aaaaaaaata gaactacaat tttatgtatt attattgcag
    15601 tatgcttagt tactgctatc tttggacttg cagatatggc tatacaatgt caaaagatac
    15661 aatttattaa agatgatgga aattggcaca tagtaattag aaatattgat gaaaagttta
    15721 tatcagaaat aaatagtaga gtagatatag ctcaccatgg tagggggcaa gtcgttgaca
    15781 gtggagagtt taaatcaaag gagattttga tacaaggttt agatgaagaa gtagcaaaac
    15841 aatttaactt agaaataata aggggtcgtt atcctattgg ggagaatgag actttaatag
    15901 atgaggatgc agttaaaagc tttaatctaa aaataggaga ttttatcaaa ataaaattag
    15961 cagatggtag aaataaaaat tataagatag taggtgaata ttcatcaata tcaagcttaa
    16021 gagctacaga taaacatgga ttaatacttt caatattggg tacacaagct attaaaaaat
    16081 cagaaacgta cgattattat tatattacat ttaaagaaaa ggttaatatt agaaaagcca
    16141 cttctgatat taaaaatacc ttcaatttaa gtgataaaca aatcggtaaa aatgaaagat
    16201 tactttctat tataggtcaa ggcgactctg acttttctaa accactgtat ttaacagcag
    16261 gattactttt tgtacttgtt ttagtagcaa gtattatcat gatatataat agttttaaca
    16321 tgagtgtaat tgagagagtt aaattttttg gtctgttacg ttgtctaggg tcatcaaaac
    16381 atcaagttaa aaaatttgtt gttttagaaa gtttaatttt aagtataaaa gctattccaa
    16441 taggcttagt attaggaagt cttgtaacta ttatatcttc tatatttctt aagtatgtta
    16501 atgagttatt cagctcaatg ccaattttaa aagttagttt tattggtatt gcatttggaa
    16561 taattgtagg gtttttaact gtaatattat cagctattat tccagcaaaa aaagcttccc
    16621 gtgtatctcc acttagcgct gtacgtggga atctaatgtc caaagaaatt ttaaataacg
    16681 ggaagacaag taaagttgca aatacaggaa gttttaaaat tgagacttct atgggattaa
    16741 atcatgcttt ttcaagaaag aaatcttttg tacttatgac ttcttcattt gccattagta
    16801 ttatactatt tttaggtttt agtgtgctta tagaccttat gtatcaagga cttaagccta
    16861 tgaagccaga agctcctcat atatctattg ttgaccctaa ttttaagtct gttttaacta
    16921 aggaagatat taatgaaata agcaatatca aaggagtaaa gaaagtatac agtagaatga
    16981 tgacggatat agaagctaca tataatggga agaagacaaa atcacaatta atttcttatg
    17041 aaaagtatca atttaattgg gcaaaaaaat atctattaaa aggagaagtt aatgaaaata
    17101 aactacagtc agaaaacctt gcattagtag aagaggggca tggatttgat ataggagata
    17161 agatatatat taaaaataga aatgctgaaa aagagttaga ggtaggaggt atactttcct
    17221 caattccatt tgaggctaaa gataaatata ttggaaatat tataacatct gaaaatactt
    17281 ttacgaatat aacaggtata aagggttata taataatgga tattcaggtt gataaagata
    17341 ctcctggaga tttaattgaa aatataagac gtgtagtaga tgataactta gaggtcagag
    17401 ataaaagaca aagtaactta gaggttaaaa attcgtttta tactatggca acatttgtat
    17461 atggatttgt gtttattatt gcgattataa gcatgtttaa tatcataaat agcatgaata
    17521 taagtgttac aagtagaaag aattactatg gtgttatgcg tgcagtaggc atatccaaca
    17581 gacaattaag aagaatggta atagtagagg caggttcata tgcaatgagt ggttgtttat
    17641 taggtagtat actaggatta atgttacata ggtatatgtt tatatcgtta gtaagttcaa
    17701 tgtttcattt agagtggcaa gtacctttta atttactact tatcactgta ctaactatga
    17761 taataataac tttattatca gtaagaaaac cagtcaaaaa gatttgtgaa ttagatataa
    17821 tagactcaat aaatgcacaa taaatatagc tgttgattta aattaaaaaa acgaagtaaa
    17881 aatgaaatag aaaatatttt tcatttttac ttcgtttttt atattataaa atttttgtta
    17941 tgttacaaaa tttctataaa gatgatgtga atctaaacat tatatgacaa cgtgagcatt
    18001 taagttaaaa tattttaaat atactatttt agataagttc cttcttcatc ttgataaatt
    18061 aatccttttt tcataagcac tccaagagct cgtttaaaat ttttcttact tgaattaaat
    18121 aatatagaaa tatctttagg gtcagattta tcattaaatc tcatatatcc atcagaaccc
    18181 tctaaataac ttaaaatctt attttctaaa gtatcaagtt catcctttct atttcctcta
    18241 ggagaaagac caagttttcc atcttcatat atttttatga catgtaaatt ttcaagaaca
    18301 tcaccagctt ttagttcagt aaagtactca ttatgaagta ttacaccagc atatttattg
    18361 tctacacata tcattgcaga attattagtt tgaaatccat atacaactcc agttacaata
    18421 tctcctacat tgtaagtatg gtctgttaat aaatatgaat ctatatctgt agtagcagca
    18481 attcttttgc ttttgtctaa atatatataa aataaatatt tttcgtctct aaatagtgga
    18541 taagtcttag ctttaaaagg aactagtatg tctttaggaa gtcccatatc tataaaagta
    18601 ccaatgtccg tattatcaac tacttttaaa tgtgctacat ccccaacctt agctaaaggc
    18661 ggtattaaag ttgcagcagt tctgtcatca gaatctttaa atatgaaagc atttacttca
    18721 tcacctatgt ttatttcatt ttttccgatt aatctattat gaagaagtat atcgtctttt
    18781 gtgttattag tttgtccatc caaaaaatat ccaaattcag tttttctttt tactgtaagt
    18841 ttattaaaat cacctatgtt tatcaaatta aaatcatcct ctcattttat atttataatt
    18901 tagccataat atacttatgt agtagtttgt cacagtatct atattagtat tagtgaaatt
    18961 tacaataaat aatatagtta tttatgtatt tttcaattct ctagcttctt atatagtata
    19021 aactccaatt tcttttttgt ctttaggttt tactaaaatt tttacaaaat aagttaaaaa
    19081 gtattgaaat aaaatttctt ttaaagtata attggtatat accaaaattg gtatatacca
    19141 tatcgaaaag tggtgactat atgaaaatag taaataaatc tagtaatata ccattgcata
    19201 ctcaattaag ttcaataata agagagatga tagaaactgg tgagcttaaa gagggtgatg
    19261 caataatgcc agaaagagaa ctttgcaaca tacaaaatgt aagtcgtatg acagtaaata
    19321 aaaccattgt tggattagtc acagaaggac ttctttatag ggtacaaggt aaagggacat
    19381 tcgttgcaaa gcaaaaaaag aagtatcagt tttcaaatgt aaaaggtttt actgatgtta
    19441 tgaaagaaaa gggagtaaac ataaaaacgg atatattatc ctttgagatg gaactaccag
    19501 atgatttagt taaaagaaag cttggaataa gtgataatac tacaaatata tataagattg
    19561 tcagacttag atatactgat ggagagccat ttggattaga aatagtatat ctatcagaag
    19621 aaatgtgtaa aggtctaaca aaagggatat tggataattc ttcattatat agggttttaa
    19681 atgaaaaata tggctataag atacaaaaag ctgaacaagt aatggagcca gtaatacttt
    19741 cagatgaaga aagtaagctt cttgaaacag atgaaggtgc tttagctctt aaacttcata
    19801 gaaattccta taatagagaa ggtagcccta tagaatatac catatcaata tttagaacag
    19861 ataaatatca atatgaaata gtattgagtg agtaggtgaa taatatgaaa tgtttaatta
    19921 atggaaagat aatattaaaa aatcaaatct tggaaaataa agtattagta tttgatgaaa
    19981 aaataattga tatagctgat agtgtaccaa aagattgtga agttatagat gctgatggaa
    20041 aatatatatc tccagggctt attgatattc atatacatgg aaatatggga aaagatacaa
    20101 tggattcgac tgatgaatct attgaaacaa tttcaaaatc aattatgaga catggagtaa
    20161 catcttttct tccaactaca atgactatgg ataaagaaca tgtttatgat gcacttgaag
    20221 ttattaaaaa agcccaaaat agaaagcttg aaggggcaca agtattagga gcacatttag
    20281 aaggaccttt tataaatgag aattataagg gagcacaaaa tgaaaaattt ataataaatt
    20341 caaaatacga atttataaaa gaatataaag atgttataaa agttataact tatgctccag
    20401 aaaaagatat tgattttgat tttactagag aaataaaaag atgtacagat atagttttat
    20461 ctattggtca tagtaatgca aattatgacc aagctaaaga agctataaat ttaggagtaa
    20521 ctaatataac tcacatgttc aatgcaatga ctggtttaaa ccatagagac ccaggcgttg
    20581 ttggtgcagc acttacaact aatgtgtaca gtgagctaat agcagataca atccatataa
    20641 ataaagattt atttcaattt atactaaata ataaagggaa agaaagactg attttaataa
    20701 cagattctat agaagctggt ggtcttgaag atggcaatta ttctcttgga ggtcagaaag
    20761 tcatagttaa gggtaatgaa gctagacttg aaaatggagc attagctgga agtgtgttgt
    20821 ctttaaataa aatggtattt aacttcttag ataatacaaa ccttaaagtg aatgaagcaa
    20881 ttaatttagc ttctttaaat ccagcaacat ctttgggaat aaatgacaaa aaaggaagct
    20941 tagaaatagg taaggatgca gatatagctg tatttgatga gaatttagat tgtaaaatga
    21001 cactttgctt gggggacgta gtatataaaa atatatagta tacaaaacaa actttaaaga
    21061 cagtaaaaac aatataaaaa ataataaatt aaacttacat tagttaaagt agtagttaaa
    21121 atttaggagg aataagatga gagtattagt gtgtaaagat tatgatggaa tgagtaaaaa
    21181 agccgctgaa atgatagcag ctcaaatagt tttaaaacca aattcaatac tgggactagc
    21241 tacaggaagt acaccagtag gtatgtatag agatttagta aaaaaataca atgataatat
    21301 agtagatttt tcagatgtga tgagctttaa tttagatgaa tattataagt taccaatatc
    21361 taatgaccaa agctatgatt attttatgaa agagaatcta tttaatcatg taaacataaa
    21421 gctagaaaat actcatctac caaatggaat ggctgatgat attgaaaaag agtgtctgaa
    21481 ctatgaagct tctatagatg cagctggtgg tatagatgta caagtacttg gaataggtcg
    21541 taatgctcat ataggtttta atgaacctga tactaaattt gcaaaaagaa ctcatgtagt
    21601 tgaacttaca gagtctacaa tagaagctaa tgcaagattc tttaaatcaa gagaagatgt
    21661 tcctaaaaaa gctgtaagta tgggaatagg ttcaatattg aagtctaaga aaatattgct
    21721 tttagcttca ggagaagaaa aagctgatgc agtatataat actgtttacg gagatataac
    21781 tccagaagtt cctggttcta tacttcaact tcataaagac actatagtta tagtagatga
    21841 agcagcagca agtaaactga atccaaaaga ttataaatta gtataagaat agaatgtagt
    21901 ttaagtgaaa aatagctatc attgaataat caaatttatt taatgatagc tatttttatt
    21961 taaatttttt acttcttttt atttaaatta gttattattg tttaaattac ttgcttatta
    22021 ctttttacga aaactcgtta tcaagaagta tccgaataag atgttaaaaa ttattatgtt
    22081 tagtgggatt ctatcaagca taataaaatt acctataatt gggatattac caattggaaa
    22141 cataaagaag aatacaggca tcagtgatag taaaaaagat ggaaaacaaa gaagaagtaa
    22201 tcttggaata tcaatatacc aactaccatc ctttttgtat tctctaatca ataattcagt
    22261 tcctagtaac aaaccaatac caccagagga aattaaatta ataaaaatgt atcttttaaa
    22321 gtcttgcata taaccagtgt gaaattgagt atagaaatca taatttaatt taacggctaa
    22381 aaaatatata agcaatagaa tgattaaaaa taaaagatat ctaagagctt ttttcatttt
    22441 aaattctccc ttcagaaata ctttattcag ttttattttg ttggaatctg attttatcat
    22501 ttagtttaag aaatccataa tattagtgaa gaatgaaata agtattatat atgaataaat
    22561 atttttagaa ttaaggtgaa ctgtagtgct taatataact ataatattaa ttgtttgaat
    22621 attcaataaa aagtttaaaa aatatcaatt ttgatataaa ctgaataaat acagttgaat
    22681 attaaattat tattttatca aaaatatgca aagtactaca ccttcaaata tcacctaaag
    22741 taattaaaag gcaattaaag tgaagtagtt caagtatttc taaaatgagt ttaataaatt
    22801 ctataataat ttattttttg ccttaaaatt gccttaaaat ccaaacaatt ttaaaactaa
    22861 tgtactataa aaagctggta taatttaaat atagtaaaaa ataagattta tcttatctat
    22921 tgggatttaa ttatttaatc aatatattta taccatttaa tttaaataaa aagtctatta
    22981 aggagtgagc ttatgagtaa gttaatttat atagcagatg atgaagataa tataagaaat
    23041 ttggtaaaga cattcttaaa aaatgaaggt catgatgtta tggattttaa aacgggtgat
    23101 gaacttttag aacaatttaa tataaaggag tgtgacttag taatactaga tataatgatg
    23161 cctggctcaa gtggttttga agtatgtaca aaacttcgtg agaaaagtac agtcccaata
    23221 atcatgctta ctgcaagaga tacagatata gattatatta cagggataac cttaggtagt
    23281 gatgattatt ttacaaaacc atttagccca atgtctttgg ttatgagagt aaaatctata
    23341 tttagaagga tagagtttga aaagaaacaa aattatgata aatatagtaa ctcgatagat
    23401 atggaattaa aatttggtga tgtaataata aataaaaaga ataaaatcgt tacaagtaaa
    23461 aatgtaaata tagaccttac accaaatgaa tataatctat tgacttattt gtttgaaaat
    23521 atagatagag cagtttccag agatgaatta ttaaacaaaa tatggggata cgatatagaa
    23581 gttgagacaa gggctgctga tgatacagtg aagagattac gtaaaaaaat tttagataca
    23641 aatattttaa tagaaactgt atgggggttt ggatttaggt tgaaggagaa aagttaagat
    23701 gaataatgga aatgacaaga atagaattgg aaagattaaa tccaaattta aattaaagtt
    23761 ctcaaagaaa aatataagca taaaaactag gattttgttg agcatacttt gtttgataat
    23821 tatagtattt atgataatat tagtatcttt taatgtattt cttgatacat atataaaaac
    23881 aactgcgaat gaagaattga cgaagtctac agaaatagta gaacatatgg atagtaattt
    23941 tagacctatt aagccaccac aagatgatgg aaagcttcct cccaaagtat tatctaattt
    24001 tatgagaaat gtacaagata aagttaggat ggcagaaaca caatcagatg ctgatgctat
    24061 ggtagttgat tctaagtata acctgatatt tccaacaaga gaggatgatt tcttaaaaaa
    24121 tgttgatgag atggagctga ttagggagtg tattcaaaat gaaaaattag atttaaattc
    24181 agatgaaaat acacggatat ctaccagaaa taaggattat tatatttcaa ctgtaaaaat
    24241 tactagtata tatggtgaac aagacaaata tcttatactt tttatagaca tatcaaaaac
    24301 tctaaatcta gcacaaaaga taaatattgt attaatatct gtaatgtgtt ttgctggaat
    24361 attagcaata tttacagctg tgattttatc tgagaaaatt gctaaaccga taaaagagtt
    24421 gtgtgagttt gcaaaaaaaa tgggtcaagg agatttcaaa agaacctact ttgacttttc
    24481 agataaagaa ttagtagagc tctcaactgt tatgaataag agtgctgagt atctggataa
    24541 gtatgataat gaacaaaaaa tattttttca aaatgcatct catgaattaa ggacaccact
    24601 catgtctatt aaaggttatg cagaagctat aaaatataat gttatagata gtaaacatgc
    24661 aagtgatata attcttgaag aaagtgatag gttaagtgat atggttgaag atttattata
    24721 catttctaaa atagataata taactaaaga ttttgaactt gtagaatgtg atttaagaga
    24781 agtattttca aattgtggag ctaggcaaaa tgtaagagct ataaataagg ggataaaatt
    24841 tatttatgaa tttgatgaag aaatggtttt atttgaatgt gatgaaaaaa acatttccaa
    24901 agcatttatg aatcttatag aaaatgcttt gagatacgct aagagtgaaa taaaaatagt
    24961 gtgtaagtat aatcaaaaaa atatagttgt gataatagaa gatgatggaa ttggaatcaa
    25021 gaaagaagat ttacctcatg tttttgaaag attctataag ggagtagggg gaaatcatgg
    25081 aattggatta tcaatagtta aatctattgt aaataagcat ggtgggcgta tatacgtaga
    25141 aaatggcaaa aaaggagcta agtttactat attatttaaa ttgtaaaaaa attagagaaa
    25201 atataaagat gtttgaagtt gttttttata tatatctgtt gtatactgtt gaaaaaagaa
    25261 caatattaag ctgacatata ttacaataat tgacatttaa agtaaatgct taaatataga
    25321 tacgaaagga aaagtaaaat ctgaagtgta tttaggttta agtaagcttc ttattttata
    25381 aaaaatatgt atgaaagaac agctgatttt taatattaca aatttagtaa cggttgtatt
    25441 tgaagctttt gtcatccata tgtttttatc agacttttta ggtcagaaag aagggtatat
    25501 aaaagttgta agatatgcta aactgggttt tataatatgt ttaggattct gtaatttaat
    25561 aactttaaac cctaaaatca caatgccttt aatttttatc cttatttttt caacatcatt
    25621 tttatataaa ggaaatttaa agacacgatt gtttactact gttttattaa gtatattctt
    25681 tatactgtca gaaattgtag ttacaagtat ttttgtttta tttgtaaaag aaggctttga
    25741 aataatgctt gaaaataata gtataagagt attggcgaca attttatcta aaattgtctt
    25801 tttgttgact tgtaaaatta tttgtctatt taaaaaagat gtacatttag atatgcccat
    25861 aaagtattgg ctccctttat ttttaatacc tatatttagt ttatttctgt ctgtttctat
    25921 ctttgatgta agtaaatttt ttagtcttga aagtttaaaa tttctaagtt taatatctag
    25981 tgttggcatt ttatatatta attttattgt attttattt
//
LOCUS       DAMJVX010000038        25388 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMJVX010000038 DAMJVX010000000
VERSION     DAMJVX010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25388
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.040"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            154..828
                     /locus_tag="PC407_001472"
     CDS             154..828
                     /locus_tag="PC407_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2496924.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDSLKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            816..1976
                     /locus_tag="PC407_001473"
     CDS             816..1976
                     /locus_tag="PC407_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2496925.1"
                     /translation="MDKISNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNINMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNPHFGMGLHISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            2193..2720
                     /locus_tag="PC407_001474"
     CDS             2193..2720
                     /locus_tag="PC407_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDF2496926.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFCSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            3006..3674
                     /locus_tag="PC407_001475"
     CDS             3006..3674
                     /locus_tag="PC407_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2496927.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLKINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            3677..6145
                     /locus_tag="PC407_001476"
     CDS             3677..6145
                     /locus_tag="PC407_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2496928.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSFISALPSAIYASRVSPKIAMAGSTTKIKRKIKREKAIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIDKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYNSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNISLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(6385..7809)
                     /locus_tag="PC407_001477"
     CDS             complement(6385..7809)
                     /locus_tag="PC407_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2496929.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMPYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANSRGR
                     FVFAVIIMALFSPFIYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMKGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(7828..8520)
                     /locus_tag="PC407_001478"
     CDS             complement(7828..8520)
                     /locus_tag="PC407_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2496930.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(8575..9351)
                     /locus_tag="PC407_001479"
     CDS             complement(8575..9351)
                     /locus_tag="PC407_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2496931.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(9348..10118)
                     /locus_tag="PC407_001480"
     CDS             complement(9348..10118)
                     /locus_tag="PC407_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2496932.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(10115..10819)
                     /locus_tag="PC407_001481"
     CDS             complement(10115..10819)
                     /locus_tag="PC407_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2496933.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTDGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(11074..11586)
                     /locus_tag="PC407_001482"
     CDS             complement(11074..11586)
                     /locus_tag="PC407_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496934.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEETYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDISNYYLVANYI
                     RCLKKYKLID"
     gene            11912..12169
                     /locus_tag="PC407_001483"
     CDS             11912..12169
                     /locus_tag="PC407_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496935.1"
                     /translation="MILNRTELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FSNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            12312..12938
                     /locus_tag="PC407_001484"
     CDS             12312..12938
                     /locus_tag="PC407_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HDF2496936.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDNRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIEVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(13188..13967)
                     /locus_tag="PC407_001485"
     CDS             complement(13188..13967)
                     /locus_tag="PC407_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2496937.1"
                     /translation="MNSKIYYFSGTGNSLVVSKILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIIKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPSDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(14035..14496)
                     /locus_tag="PC407_001486"
     CDS             complement(14035..14496)
                     /locus_tag="PC407_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2496938.1"
                     /translation="MGGAGDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(14472..15149)
                     /locus_tag="PC407_001487"
     CDS             complement(14472..15149)
                     /locus_tag="PC407_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2496939.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            15382..15768
                     /locus_tag="PC407_001488"
     CDS             15382..15768
                     /locus_tag="PC407_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2496940.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            15771..17570
                     /locus_tag="PC407_001489"
     CDS             15771..17570
                     /locus_tag="PC407_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HDF2496941.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVIILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSIIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLKQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(17925..19799)
                     /locus_tag="PC407_001490"
     CDS             complement(17925..19799)
                     /locus_tag="PC407_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2496942.1"
                     /translation="MNKYNKIANELIEIIGENNIISITHCATRLRVMVKDREIINDKK
                     VEKVNEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEVEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIGLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(19804..21468)
                     /locus_tag="PC407_001491"
     CDS             complement(19804..21468)
                     /locus_tag="PC407_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDF2496943.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDATPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSKYSDCLIYGSIEPIDIESDNVIAYRRQLDLVVIDCY
                     YNFSDKLVEIKTEEKYDIVFQNLDDINLLNKTVSLKPFQAVLLKVR"
     gene            complement(21482..22303)
                     /locus_tag="PC407_001492"
     CDS             complement(21482..22303)
                     /locus_tag="PC407_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2496944.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSIGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDVHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(22402..23166)
                     /locus_tag="PC407_001493"
     CDS             complement(22402..23166)
                     /locus_tag="PC407_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2496945.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVVKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDTSTIIDFAKEMRMKGTYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(23163..23912)
                     /locus_tag="PC407_001494"
     CDS             complement(23163..23912)
                     /locus_tag="PC407_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2496946.1"
                     /translation="MRLESLVNQSYNSLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKSGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(24416..25021)
                     /locus_tag="PC407_001495"
     CDS             complement(24416..25021)
                     /locus_tag="PC407_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2496947.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMKIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            25200..25298
                     /locus_tag="PC407_001496"
     CDS             25200..25298
                     /locus_tag="PC407_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDF2496948.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFD"
BASE COUNT         9103 a         4094 c         3100 g         9091 t
ORIGIN      
        1 ctaataattt catccttgat ttatttaata tataaaaata tactaacttt aatgattaag
       61 ttttgaaata taactaagaa tttgagaatt tattgagatt tttatactaa aatataaata
      121 ctatacaaac ttaatttaga agggagcatt cttatgaaca ataaaatact cgtcgtagat
      181 gatgaggaag gtataataac actcttaaaa gattactttg aaatgaatgg ttatgaggtt
      241 tacactgctc aaagtggaaa agactcatta aaaaaaatat ccaaaaatcc agatatcatt
      301 ctacttgata taaacatgcc tgaaatagat ggaattgaag tttgtaatag aattagagat
      361 tatgtatcat gccctattct atttcttaca gccagaattg aaaatactga caagataaat
      421 ggttttcgtg ctggtgctga tgattatatt gtaaaacctt ttgatttaga cgaacttgga
      481 gctagagtat ctgcacattt aagacgtgaa caaaggaagt caaatcaaac taaaattaaa
      541 ttttataatg atttaacaat agattacgaa aaaaggttaa tttgtatata taataatcag
      601 atttcacttt caaaaaagga atttgatata gtagagcttc tctctatgaa tcctggacag
      661 gtatttgata aagaaaaaat atatgataga gtctggggat acaatagcaa tggaaatagt
      721 gatgttataa tggaacacat tagaaaaata cgaatcaaac tatcaaaaca tactcttgaa
      781 aattatatag aaacagtctg gggagttggt tataaatgga taaaataagt aatatgtcat
      841 taaaaaaagc tttctttgtt cttactttat cttcactaat tatagcatca gtacttacta
      901 ctttcgcata cattttactt aataatctat ataattctat acaagataag tatttaaaaa
      961 cacctataaa tacaggtatt ataaccatag ttcaaaacaa tgtgaaccaa tttagtgatt
     1021 atgataaaac attacttaat ataatcaaca tacttcagct tgtacttcca cttatatttt
     1081 ttataggact tttattatta gctgatataa ttttttacaa ggtaaaatta aaaacaccca
     1141 tagaaatcct caataaaggt gcaatggaaa tatccaacaa caatttagat ttttgcttag
     1201 aatataataa taatgatgaa ttaggtaatc tatgtaatgc ttttgaaaaa atgaggtctg
     1261 aactaaataa aaacaacata aatatgtgga ctatgattaa tgatagaaaa caacttaatg
     1321 ctgctttttc tcatgattta agaaatcccc taacagttct aaaaggttac tcaaactatt
     1381 taacaaaata cataccaaca ggcaaactta gtgatgagaa gattttatca accacacaac
     1441 ttatgtctga gcatattaac agaattgaat actatgtaga aaatatgagt aatgctcaac
     1501 gattagaaga tttagttgtt tcaaaatcta tgtcgaatat aaacaagttt atagaaaact
     1561 tagatgaaaa tatatctatc attgctaaac aagaaggaaa atcttttaca cttaaaagtc
     1621 aaataaataa tataaatcta ttttttgatg aaaatatcat tcttagagtc gtcgaaaata
     1681 taatatcgaa tgcttttaga tatgctagaa ataatgtatc catacttata tatcttgaac
     1741 aagaactcct tacctttgta attgaagatg atggtatagg atttagtaag gaatctctaa
     1801 aattagcttt aagacctttc tatagagata agactttaaa tgacagcaat ccacactttg
     1861 gtatgggtct tcatatctca aaaatattat gtgaaaaaca tggtggctct atttctattg
     1921 aaaataattc tactaacagt gctaaaatta ctgctaaatt ctcaacaaga aattaaaatg
     1981 attttgtaag ctttcattgt tcttatataa gctttgttag tttagaattt ataaaatgta
     2041 tttcaattta caaccaatat atatactatt ttgtagaagt tgtaagagtt gcttcaatta
     2101 taatattttg gcaaaaggca tttgaaacaa taatagaaat atatatttaa atatgtagaa
     2161 tagtatgaaa ctagaatttt ggaggtgatt atattaaaag ttttgctaat aatattacat
     2221 tctctcgtat tgtgtttgca ttgctaatgc ttagttctac attctgttca ctgcctttct
     2281 ggatatgtta tttttggtgt ggaattagtg atatacttga tggtcttatt gcccgaaagt
     2341 tgagacaaca aagtggaata ggtgcaaagt tagatagtat tgctgatttc atttttgcgg
     2401 ttacaatttt ggttactgtc ggaaaaaata ttgctcttcc catgtatcta tggtggtgta
     2461 ttgttgcaat agcattatta cggttgatta gttacagtat tggttttttt aagtatcgca
     2521 cattttcatc gcttcatact ttgatgaata aaatcactgg aatttctatc tttgcttttc
     2581 cattactcta tatttgcttt ggtcttgatg ttgcaggaat tatgatatgt atcgtggctt
     2641 ttctttcttc ttttgaagaa atagcaatta tgataaaatc aaaagaatta aatcgtgata
     2701 taaaaagtat atttatgtga caacttagaa tttgtgacaa aaataattta tataataatg
     2761 aggaagtaaa acataaaaaa ttatccacaa gtttatgtgt aaaatagttt taaagcaata
     2821 gagttgtatt gtaaagcctt tagtgcataa attacatttt aaattaaaaa tgatactgaa
     2881 actgcttatc agcactgcga attatccgta aatagtgaag actttttaga tttaagctaa
     2941 aagttgggaa aaaattgagt tttaaaactt acactcttct tatatagaaa aggagagtgt
     3001 tttttatgat tataaaagca aaacaattat caaaaatata tggttctaat aacaataaag
     3061 ttatagcact taataatgta aatttaaaaa taaactcagg tgaatttgtt tctgtaattg
     3121 gaccttctgg aagtggaaaa agcacacttt tacatatttt aagtggatta gataatccta
     3181 cctctggaca agtgttactt gatgataaag atatatataa acacactgaa aaagaactat
     3241 ctgcacttag aagaaaatcc tttggattcg tctttcaaca atttaatctt ttacctgttc
     3301 ttacagcatc tgaaaatatt tcaatgcctg tacttctaga taaaaaacaa cctgataaag
     3361 actatttaaa tgaaatttca tcattacttg gaattgcaga tagactcaat catcttcccc
     3421 atgaactttc aggcggtcag caacaaagag ttgcaatcgc tagagcactt attgcaaaac
     3481 cagatataat atttgctgat gagcctacag gaaacttgga cagtaaaagt ggcagtgaag
     3541 ttatgaatct tcttataaaa acttcaaaac agtttggaaa gactttagtg gtaattactc
     3601 atgatgatag aattgcaaaa cttgctgata gaaaaatttc aattattgat ggtgttctta
     3661 tggaggtgaa atagaaatga agaattacct ttcactttca ataaaagaat taaaaagcca
     3721 gaaacttatg actacattca tcattatagc aatagtacta tcaactatta tgacaactgt
     3781 ggttggacag tcaattggta tcctacaaaa tttaagaatt gaacaagccc gttcttttaa
     3841 tggtgataga catgtatcct ttcaccaact tactaaaaat caagttgatg acataaagaa
     3901 agatgataga gtttatcaag ctggtacttc aataactatt ggcagtagta aaataaagga
     3961 ttctggtatc tctgtacttg taaaagagta tgataaaaca ggactttcta actacccaaa
     4021 attaatgaaa ttaaaatctg gacatttgcc taaagataaa aacgaaatag ccttagatga
     4081 aaacactcta aagcttatgg gtataaaacc aagactagga gtgactatac ctatgaattt
     4141 agatatttca cttttaaatg ataccatacc accatataat tacacagcta actttaaatt
     4201 gagtggcatt ttagaggatg attatacagg atatgtaagt ggtatagtaa atggtattgt
     4261 aggtaaaggt acatcagaaa atctattacc taagagatat attttatcct ctttggattt
     4321 taaaataaaa cagcaagaaa agtttcaaga aatagtaaat caattagcta aaaaaataaa
     4381 tctttctcac aattcaattc aatataattg gatttactta aatgctctta agatacagtt
     4441 tgaaaaagat gaaaatagtt ctaatagtga tggtatatct atgattatat tggtatcttt
     4501 atttgttgcc ttattggttt tactggcttc tggacttgta atctataata ttttaaaaat
     4561 ctctgttaca aagaaaatta aagagtatgg atgtttaaga gcaataggag ctgaaccaaa
     4621 ccagatatat aagatagtta ttttacaaat cttaatttta tgtactgttg ccatacctat
     4681 tggagctgta attggaatta tctcttctaa aggcataact ggaatggtca ctaatatctt
     4741 aaatccagat atacttctag caaatgataa taaagaaatt actgagctta tacataaaaa
     4801 tacaacagca tatatgttcc cattagtatt aagcactaat gtatctttga ttttttcatt
     4861 tatttcagca ttaccatcag caatatatgc ttcacgtgtc tctcccaaaa ttgctatggc
     4921 tggtagtact accaaaatca aaagaaaaat taaaagagaa aaagcaataa aaaactttga
     4981 aagacatcta gcatggctaa acttaaaaag aaataaagga agaactataa taactatact
     5041 atcattattt atgagtataa cagtattcgt tgcattgagt gagttctcta atgtattaga
     5101 tgtatctagg tcagtaagca atttaaaaga aggagatttt tccttaacaa atgaaatatc
     5161 tggttttgat aaatcaacac tagataaaat agataaaatg aaaaacgtca atcgcacttc
     5221 ctttataaag tactctgaat acaaacaggg tgaaatagat acagatataa attttgaaaa
     5281 tagtggtgag atgttaaaaa tcattggaat tgacgagcag actttaaaag acttaatgcc
     5341 ttctattaca gattctatac tagaagactt taaaaatggt tctatctgtt ttataaaaaa
     5401 tccgattgct atttctacac caggtgtaaa aactagacat acaaatttaa aacctaaaga
     5461 tagtatcaca atcaacaaaa aacaactaga tatatatagt acagtagata aaatgttctt
     5521 tttacaaggt aatggctgga taaatggtgt tgatgttatt gtatataatt ctgtttataa
     5581 caccttgaca aataaaaaca agttcaacca gataaatatt tatgctaaag ataaatcaaa
     5641 attagaacaa ataagattgt ctattgaaca gatttgtgaa aataatcctg gctctcattg
     5701 gatatcatat attgaatcag ataagcaact taaagagagt tttaaacaaa tagaatttct
     5761 agcatgggca gttatattat ttgtgggatt gattggtact ttaaacatca ttaacacaac
     5821 tcatacaaat ataaatacta ggactaatga gattggagtt aaaagagcta ttggtatgag
     5881 taatattagc ttatataaaa tgtttttatg ggaaggtgtt tattatggaa tttttgctgc
     5941 tatatttggt tcaattgctg ggtatgccag tgctattatt ataaacatgg caactataga
     6001 aaagcttgat ttcactaata taccaattac ttcaatttta caagctacta ttatttctgt
     6061 acttgcatgt attatagcta ctcttattcc gttaagaaaa gttaaaaaga tgaatataat
     6121 tgactgtata gatatcaatt tataactatt ttacagataa taatataaat taaattatca
     6181 atgactgtct ctattttctt tcaaatctaa ctaataaatc tttttaacta taatataagc
     6241 tttaaaaaag gcctgtctca aaatagagat aagccttttt tcattgtcgg aataatgaaa
     6301 atatatttaa cttcacaaaa aaagagtgcc tcacgaatta aaaatttcgc tttgagacac
     6361 tcccctttaa tttaaaatat atagttaaat gctatgccaa aatttcacac atgctccacc
     6421 tgattttgaa ttttctattt taacagaacc tccaagctgt tccactaatt gtttgacaat
     6481 gtaaagccca agaccagaat gtccaccttt tgtctgccta gcttcatctc ctcgataaaa
     6541 cttatcaaga gcctttttta aatctttaga gctaaaccct ctaccagaat cacaaatctc
     6601 ataagaaata caattttttt catcctttac agtaatacta atattaccac ctgatggagt
     6661 gtattgcaga ctgttggata tgatattatc aaaaattcgt gtcaacctat ccacgtcgat
     6721 ttgaattggg gattgtatat tccccttcat tttcaatata agttcaactt ccttttgatg
     6781 agcctgtaat tcatagtctt gcaccttttg cctcaaaaat tcaggtaaat ttataggaac
     6841 aagatttaat tgtatatttg acttttctaa gtcagacgta tattgcatct tttggacaag
     6901 agcagcactt ttttctgtat tttctcttat cactgtaaga tacctatgaa gtttttcatt
     6961 gtcatctgta ttattaccaa taagtgcatc tgtatatccc aaaataatag aaagaggaga
     7021 ttttaaatca tgtgctagag cttccaccat ttcaacacgt tcctgctcca tcttccactg
     7081 cgcagataaa gaacctttta attcgtcttt catttcagaa aaggcactac aaagtttacc
     7141 gagttcatta tctgaataat agtcgatttc aaaatcaagg tccttttctt ttatcttctt
     7201 ggacgcatca atcaatagat gtaatggttg attgatattc tttacaaagc gcttagaaag
     7261 ccatctagta aaacccacga tatatataaa tggagaaaac agagccatta taataactgc
     7321 aaatacaaat ctccctctac tatttgcaaa ggttattttg acttgataga atagaataac
     7381 agctccctca atctttccat tatccccttt gattggaaca gtatggatat aaccaccttt
     7441 acgaacagtt ttattgataa aattattaaa taattcttct tttgttttaa atagtttttt
     7501 ggggttagta ccatatagaa tattgccatt attatccaca acttgatatg gcatatcatc
     7561 cccacgaatc gttcttttta aaccttcttc attcgattgt gataacaaag ttatgttttt
     7621 ttcattgatg tatttttcaa ttctaggaac ttgtcgctca taatagtttg gtggatagat
     7681 atccttattt aaggaatgaa tatacaaata gagagcaaat acataagtaa tcactgtggc
     7741 aactatactg gctattataa taaaaataaa agtatgtcta aattgggtaa taagggattg
     7801 atttttcatt taataacctc tctctcttta cattttattc cacttatacc caatacccca
     7861 aactgtggaa atatattctt tttctggtgt cacagcagaa aacttagctc gtattttttt
     7921 tactcgttca acaacagttg aggaatcacc ttcagaatca cacccccata ctttttcata
     7981 gatttgttct cttgagaaaa cttgtcctgc atggagtgct agtaattcaa caatatcata
     8041 ctctattttt gttaaatcaa tattcttacc atcaatttta attgcacgtt ccttcatata
     8101 taagcataac ttcccaaaat acaaagtaga tctcttgttc tcttcattga tatactgtga
     8161 acgcttttcc ctacgcagat tagcttcaat ccttgccata agttcacgca gtccaaatgg
     8221 ctttgttaca taatcatccc ctccaaaggt caaccctttg attttgtcgg tttctgattg
     8281 ctttgcactt aaaaatataa ttgggcataa aacatcatct ctaattgcct gacatacctc
     8341 aaatccattc attttaggca tcataatatc cagtataata aggtcaggct cctgttttga
     8401 taactctata cctgcctgac catcataagc tatcaacact tcatgtcctt ttgccttcaa
     8461 ttcatcctca agtagcatta ctaggtccat ttcatcatca ataatcaata ttttactcaa
     8521 tataacacca cctttctctt atgggaaggt cagtattaaa acaatattcg tctactaata
     8581 acttttcctt ccctcccaca ttttaaacca aataacgcct cctatcaaga atataatcaa
     8641 acctattgct actactataa gctctatact agctgtttgc atcactttag agtaaaatgc
     8701 ctcagttgaa atagttccag cttctttaat aaaataaatt cctggaagca tacctagttt
     8761 tactggccat gtccatggaa caaatgccca tactttttca ccaacgctag ttaatccaag
     8821 tattgctgcc atcaaaattc caccgatact aataccaata gatgcaccca taccccatgc
     8881 aaaactcacc caaagatgaa tagctaatag tggaagtgtc ccaatcacca caagtcctgc
     8941 tgctgacagg aataaccata ttgttgctcc agatggcact gcaatactca ttcctatgca
     9001 aagtatcaac gtagcaataa atgtgcatat agttaggcaa aacagaagaa aaagaaattt
     9061 tcccagatat aatctaacac gagaaatacc tacacataaa aaaccattaa aatttccagc
     9121 aagctcttcc tcctgcacta taaatcctgc taaaatacct actccaatag gaatgatgaa
     9181 tacagtccat actgtaaaaa atccttcaaa agcaaattct tgtgtacttc cagatttagt
     9241 tgtgagataa aaaaccacac ataatgaaaa aataataggc atacaaagag taagccactg
     9301 gacagctgtc ctctttgctc gaagccattc tgatgataat agctttatca tttgtcatcc
     9361 ctcctattga accaagctgc agtaagcgct gttaatacta taaacacaac caaagaaaca
     9421 acaatcccaa taggaatcac tgaagtattc aaaagtgggt ctccagcttc taatatagta
     9481 ccgttaggat gtatgttgat gattgggcat accattctaa ctgcccaact ccaaggtact
     9541 gcaatccaaa atggttttgt cgcagcaagg acaccaaata tcatacctaa agctccaact
     9601 cccatactta ggaacatacc tttccatgta gctagccata gctgaagtgg aagtatagca
     9661 agagaaacaa cccagcatac aatacttgcc cctataatct gtccaaaagg aactggacca
     9721 gctttcaata ttaaaccagt tataattgtt acgactatta aaactaacgt tgaaataaaa
     9781 ctgtagactg ccatagctat aactttatta atccaaagtg tcataggaga cacattgtgt
     9841 gtacgtaaag cacgataatt tccagctttt ttttcctgga aagctaccaa agtagcaaac
     9901 aatgcaaatc caataggcag aaataaaaat gaccaccagt taaaaattaa tgctaaaaga
     9961 ctttgccacg cccaagaagt tgtttgagat aatggatttt gcatgagtgt tgattgcatt
    10021 ataaatgcat atgctgcaaa agacactggg aaaaaaacaa taagtttacc cataaatgtt
    10081 cttttatact ttaaaagttc tgattgtaaa gcattcatct gttttgtcct cctttaagaa
    10141 taacatctgt aaaaaattct tctagatttt cattagggtc aggcattcct tgaaatagta
    10201 gtcgtcctcc atttataatt cctatattat ctacaacttg agcaacctct gatagaatat
    10261 ggctagaaag aattacagtt ataccttttt ttggaaaaga agcaatcaat tcacgcaatt
    10321 cttgaatgcc aaaggggtca agaccattgg ttggttcatc tagtattaac aactctgggt
    10381 cactcaaaag tgcaatagct attcctaacc tttgtttcat acccatagaa aattgtgaag
    10441 ctctcttttt acctgtattt ttcaaattta ctatttccaa cacttcataa attttatctt
    10501 taggtattcc caatattttt gtatgtacta acaaattttc ctctgctgta aggtttccat
    10561 acaatgcagg ggattctatt aaagaaccta tttttgcaag agattctcgt ttccaaggct
    10621 ttctatcaaa aataatacga ccatctgttg ggcgaagaag accaacaagc attttaagtg
    10681 ttgtggactt tcctgctcca tttggtccta aaagtccata tatactattt cgttcaattt
    10741 gtagagaaac attgttaact gccatttgtt ctccatatct ttttgataag ttttttgttt
    10801 caagaattaa atctttcata ttaatttctc ccttcttgta ataccttaaa tctattacta
    10861 agagaatact aacatgaaaa aataacaatt ctataacacc tactaatttt aataaaaatt
    10921 aaaataaaaa atagagttac caattttatt ttggcaactc tatttaagtt tatgttttac
    10981 agttatatta gatttaagct tagtttcatt atcacactaa cttcaaattt actctataat
    11041 ccttaaatca agcactaatt tattaatcaa acactaatct attaatttat atttctttag
    11101 acatctaata tagtttgcta ctaaataata attacttata tcatatttat ctaatccttt
    11161 aaaagaagtt atttcatcat taagctcatc actaccttct acaaaaacac aacctgtttc
    11221 aaattccata cctacaataa tcttacagcc actataagtt tcctcttgag tatcgtacaa
    11281 tttttttgca tttccttcat ttggaattgt aaatacaata ggttttaatt ctttatcact
    11341 ggaaatcttt tgtgaaaaag gcttgtttaa cttaatatta ttgataacta catgtttaaa
    11401 ttcctctgaa gtttcttcta ctattacata ttttcgcttt aaaaactcta ttatttctaa
    11461 aacagactta gaatttactt ttaaaatact tttatatttt tcatataaag atttccactg
    11521 ttcaattttt tcctgtggta cttcttggat atgtgtactt attttttctt gagatatttt
    11581 tttcaattaa actctccttt caatacttaa atatccttat aaatctcaca tgactaaagt
    11641 tacaaggttc ctacttaata gaattttgca tacttaaata tgtcttatta attctctaca
    11701 ggatatctcc gtagtcccta cgattcttac ataagttacc tattttaaat tgttattagt
    11761 tagattataa aaattattta ttttatcact aatataatac tcttaaaaac tttacaaatc
    11821 aatttaaatt tgctaccttt tatttataac aacttatagt acatttctcc tatgcttttc
    11881 aatatattac ttttaaaagg aggttttcct tatgatttta aatagaactg agcttttccg
    11941 aggatattct ttaaatagat ttaacgagat atgcaatata ttatcagtaa ataaaattaa
    12001 atataagtat aaagtgaaaa ataatgctta ctctaaaaaa cctttttcta atgaaataac
    12061 acttggcaat ttaggtcaaa aggaagattt ctcttatgag tacttaattt atgttcataa
    12121 agacgattat gaacaggcag agtctctaat aaattctaag ttgcgataga ttatttataa
    12181 aacatgacat tctgttgtca tgttttatat gttattatac tttcaaatat gctactatat
    12241 tttcaaaatt gtgtgcttta aaactagctt tttatagtaa tttaaagctt aaaactaagg
    12301 agactcgtta tatgaaaata aatataaaat caaagttaag tgaatttatt aaacagaaca
    12361 atctttttga tgacaacaga ataatatcct accttcctaa tataacaata gaaacagaca
    12421 aaataaaaac tataatgatt aatgaggttg tcccaactaa caccaatgac gatttttata
    12481 gtgtagataa agatgctgat tatttaaaaa ctactattcc tttatttgac agtgcaggaa
    12541 tagaagtatc taacataaat gatatcctag atatgggtat ctatataact aatgcagtaa
    12601 aacttcctaa atcagaatat acaataactc gtgatacaat taaattacat atgcctattt
    12661 tagaagaaga aattaaatta tttaaaaacc tagaggtagt aatgttaatg ggagatgttg
    12721 caaaaaaatc ttttaatatg attactaaaa agcatataaa aaagaatgtc atcccttcca
    12781 tttcaacata taaacttcgt aataatgaac tttattatga taatttaaga gtatttccat
    12841 catatattat gactggtgga aacatactga ttgaaaaatc aaaatttgaa atggcatcag
    12901 aagatataaa aaaaatgttc gagattataa acggttgata ttacaaaata ataaaaacta
    12961 atttagccaa ataataagtg cctcaaaaac ttctatttat gaggcacttt tattttatat
    13021 tagtgtaact atatactatt aacataattt attatttata ttgttctttt taatctctat
    13081 tccatttagt ctaggtaaca ttactcttta caagattact ttatatttct tttatatctc
    13141 taacgttttt atttaataaa acaattatta atacaagtca aatatatcta tttatctaaa
    13201 aatatatctt ttacagtaat atctggatgg tgatacttct tcctattcgt taaaaaagat
    13261 ttttttacat taatagcctc ttttggacac cagtgaaaac aagctaggca tctttcacac
    13321 tcattacccc actttggctt tttatcaact atacttatat tctctacagg acaaaccctt
    13381 ttacatgtcc cacatccatt acatttattt tctgcaaaaa atttagtagt tgcaatttgc
    13441 atattcttac ctactaaacg aagtacaaca ctatctatat gtgactttaa attgtttttt
    13501 ccttctatat ttccaatctc caagttattg atacattttg caatctcagc taccctatta
    13561 gtctgatttt tcaatctttc ttcttcaaca tctgaaggtg ttatcaaagc atttccaggc
    13621 atattgatta aaaaaccagc agataatttt ttcccccttt ttttaagtaa cttatctata
    13681 gtaaataaac tatgtccagc taccccatta caagtggcaa tggcatatat atgagtatta
    13741 atattaaaat ttaatttctc tacaaaagac tttattatat caggaacatc agcaaaataa
    13801 acaggaaaaa caattaccaa tatatcttca tttatgtcta tattattttc ttttctatgt
    13861 atagctaaag gtataacttc tttacttcca tttaacgttt cttttaaaat ctttgaaact
    13921 actagcgaat ttcctgttcc agaaaaataa taaatcttac tattcattct tatccctctt
    13981 tctatatttc tacatcatca atagttaaaa agtctcttaa aataggattt atatttactt
    14041 tccaattcta tccaaaatat cagtaaacaa aggcaattct tcatcagaga tagatttaaa
    14101 tgtctctgtt gcatatatac ttgcaatttc atttaatggt tcttctaatt ctctacctaa
    14161 atctgttagg aatatttgaa acttacgtct atctttaaca tctactttac ggacaattaa
    14221 tcccttattt ttcataacgt ctaaaattct tgttgtatta gatttatcct tacatgattt
    14281 ttcacataaa tctatctgag aaattccatc ttgctcccat agaaacctta ataacgcata
    14341 atggtcagtt gttatatcat atccattttg tttaaacatc ttttttaaat catcttttac
    14401 ttttaacgaa gtacttacta acttagttgt agcttcatat agtttctcaa aatctttcat
    14461 tttacctcca tttaaataat cccctgctcc tcccatctat caattattcc cttatattgc
    14521 tcaatatctt taaaagccat tggctttaaa acagcattta tagcaataaa attatagatt
    14581 tgagaaagca caggtggatt tttcttttcc tctatgcaat aaataaattt tttagatacc
    14641 ttttgtatct ttgattctac tttttcagtt ataggatttt gattaaatcc agtttttata
    14701 gataaactac cacaaacata acctcccgaa tctaagacca tggattttat aaaagaaatt
    14761 gcttttgaac cacgtgcatt ttcatatgta gtaactgcta tacatggctt acgatacaat
    14821 aatctttcaa gcatcatatg tgctctatct gtaaacacct tgaaaagtcc agttacacta
    14881 cttccataag ttgggctacc aaatataaca ccatcactct tagtaattat atcatgaata
    14941 tactctactt tatcattttg attgatacac ttgcccattt tataacaatt taaacaacca
    15001 atacaatatg atggatttac ctcacttaaa tcaaaataat taatttttac atctggtatt
    15061 aatctttcta tatctttata caagtaagtt aaaacttttg atgtagctcc attttttcta
    15121 gggcttccat ttataactgt aattatcatt tctatatctc ctttttagtt gttatatcaa
    15181 caataagtat atcaactata tttttagctg tcaatataaa ccatttattt aaagctatac
    15241 ttacacagct taaataaaag ttttatttaa ctacatgtgc ttatttaatc aactataaat
    15301 gaattatttt taataattga caaactaaat taatgataat atactttatg tattacaaat
    15361 gtaagtagaa ggaggtatag tatgaaaaag ttaccacata tttcagaagc agaatatcaa
    15421 gttatgaaaa ttatttggaa atatgctcct atcagcacta ctgaagtaat tgaaaagttg
    15481 gtagaaacaa gtacatggag tcctaaaaca attcaaacaa tgttattgag attagttaaa
    15541 aaaggagctt taacatatga aaaaaatagc agagtattcg tttatactcc acttgttaaa
    15601 gaagaagaat acgtagctac agaaagtagt tcttttttaa atcgttttta taatggtact
    15661 cttaattcta tggtactaaa ctttttagaa aatgataaac tatctgaaga tgatattgag
    15721 gaactgagag aaatcttaaa taaaaggacg actaaggggg ataaataaat atgtatgccc
    15781 cattctttat acgattttta ctcagtacat taaccttatc aatattaatt gttataattt
    15841 tacttgccaa aaaagtattt aaaaaacata tttctgcaaa gtaccaatac aatattggat
    15901 ttatattgct tataatgtta ataattccat ttgtccctct taaatatatt aatcttggaa
    15961 atgtatttaa ctatttatat acttttaatc aacttggaag cagtactaat aggacttcta
    16021 ttataaaaaa tagtccaaat tctataatta ataactcaaa tgcattaaac gatttttcag
    16081 tatccctaaa ccaagttaat tttgatagtt taaatatttt attactaata tcatggataa
    16141 ttggaatttt aatcatgata gctattacta ttcattgcaa tattaaaata aaaaatatga
    16201 aaaaatcaat acaaatttta agagatgagg agatagtatt tatatttgaa aagtgtaaaa
    16261 aagatattgg tataaataag gattttatac tatgcaaatc acctattata aatacaccta
    16321 ttacatttgg atactttaaa ccacacatta tattaccaga taagtctata tctaaattat
    16381 ctttaaaaga tataaaatat atcttacttc atgaattaca acactttaaa aataaagata
    16441 tactgataaa ttatataatg tgcttccttc aaatattata ttggtttaat cctcttgtat
    16501 ggtatgcatt taaagaaatg cgtatagacc gtgaaattgc atgtgatatt tcagttttaa
    16561 aaaagcttga taaagatagt catatagaat atggacaaac tctaataaac tttgtggata
    16621 atatttcaag accatcaaat cttacactta catcagatat tggtgggtca aaaaaacaaa
    16681 taaaaaagcg actattgaag ataatggatt ttcataatga atcaaatacg ttaaaattca
    16741 aaagcatttt aatttttaca ttgatagctt taattgtatt tggaaatatt cccaaactat
    16801 ctattatggc agatatcaac gaaaaatata gacctacaaa tgaacaaact gtgtacgagg
    16861 acttaagtca gtattttaaa ggttttgatg gaagttttgt tatgtatgat ttaaaatcca
    16921 agcaatatca tatttacaat aaagaaaaaa gtaaaacaag aatttctcca aattctactt
    16981 ataagattta tagtgcctta ttgggattgg aaaatgggat aataacaaga aataattcac
    17041 atatcccttg ggatggaaag catcattcta atagttcttg gaatgaagac caagacttat
    17101 tttctgctat ggaaaactcc gtaaactggt actttcagac tctagacaaa aaaacagggc
    17161 taaaaaacat acaaacttat cttaagcaaa ttggatatgg caattatgat gtttcaggtg
    17221 gtatctcaaa cttttggcta gaatcttcat taaaaatatc acctattgaa caggtagaat
    17281 tacttgaaag tttttataca aataagttta aatttgaaga taaaaatata gaaacagtaa
    17341 aagattcatt gttaatatca aaaaaggatg gggtatcact ttttggtaaa acaggcactg
    17401 ctaatataaa tggaaaagat gtaaatggtt ggtttatagg ttatgtggaa aaaaatgaaa
    17461 atacttattt ctttgctaca aatattcaaa gcaaagatta ttctaatggt agttcagcat
    17521 ctaagataac attatccata ttaaatgaca aaaaaatcta taatccttaa tatatttact
    17581 gtacattcta aaaaattcac ataattttca tattaattat cgattaaatt catatacaag
    17641 gcttattata taaacataga agatagtaaa actagcttaa aaacataata taatacccta
    17701 tacaaatagt ggcaactctc cctcacatcc tcaattgttg ccactattta attttttgta
    17761 tgaagttaaa tatattttca tcatttcaac aatttgggtg cagtacaatc tctattttga
    17821 gacaacctct ttaattgttt tagagtttat gaattatttc tcatacttta aaaagattga
    17881 attactcttg ataatcttct tttgtagtca ttacatatca cctactactc aacacatatt
    17941 ctttgaccta tttttacatc cttgtttata tccaaaatat ttaccttctt accatctgga
    18001 aacaatacca ttgtcaaagt agactttcct aattcatgta ttctctttaa atcaacatta
    18061 acaagaacat ctccctgttt aaccatatct ccttgtttaa cagcaatatc aaagccatct
    18121 ccatttaatg caactgtatc aattccaaca tgaattaata cttctccatt agttccttta
    18181 atcccaaatg catgtccact tgaaaatact gtttctaatt tacctgaaat aggagaaaca
    18241 atttttccat ctataatatc aatagcaaaa ccatcaccta acattttttt agaaaatgtt
    18301 tcatctggta cattttctaa tgcaacaatt ttccccttag ctggagacac aaagttttca
    18361 ttattttcat taaacactaa ttcttcgtct tcatcttcat ttatagcttc tttaggcact
    18421 ccaaataaac atgttacaat aaagcagaat gcaaccacaa atattgatac taataagtat
    18481 gtgattaaat tatatccatc atagatatac attaaataag aaggtaaaac tgctaatcca
    18541 tatgaattag attgaatatg taaaattgat aatattgctg ctcctaaacc tgaagatgca
    18601 cacataataa tcaaaggctt taaattatat cgaagctgaa tcccaaataa aacaggctca
    18661 cttataccaa aaagttgaga tagcattgct ccaactgcag tttgtttaac ttgattattt
    18721 ttagattttt tcataaatgc tagacatgtt ccaatattcg caaatccata cattgcacaa
    18781 agagtaataa gtgcattaaa tcctgtattt gctaatagtg atgtttcaat cattacataa
    18841 gtatgatgta agccagtaat aacagctaat ggatatgtaa atccaataat aaatcctcca
    18901 attcctaaag gtaaaccaat caataaactg atgatatcaa ccatttttaa ttctattgta
    18961 tgcattatag gccctacacc taacaaaata actaacattg ttattagcat aacaaagaat
    19021 ggtgtgaaaa taaggtctaa tacattaggc atatgtttac ggaaccattt ttcaagatta
    19081 gctccaataa atgctgtaac aatagctgtt aaaacacttc cttgacagcc tacaatagga
    19141 ataaatccaa atgccataat tgcttcaact tcatttgatg ggtcagcaac agaatatgca
    19201 ttaggaagta ttggagaaac taacataagt ccaataacaa gtccaataac aggtgttcct
    19261 ccaaatacct taaatgctga ccatacaata attgctggta aaaaagcaaa tgctgtttct
    19321 gtaagaaccg aaacaagtgt ctgaatatac aatggaatat ttgcagaaga cattccaaat
    19381 aatccaagta cattatcatt aaacaaacaa ccctttaatc ccaagaataa tcctgttgcc
    19441 gcaataactg gaataatagg aacaaatata tctgctagag ttctcataac tttcttaaat
    19501 ccagtttcat tatttttaac taattcatct tgttcttttt ttgataaagt tttcaatccc
    19561 attttttcaa cttctgcata aactttattg actattcccg ttcctagaat aatttgatac
    19621 tgtccagaag taaagaaaac acctttaacc tcattaacct tttccacttt cttatcatta
    19681 ataatttctc tatctttaac catcaccctt aatcttgtgg cacaatgtgt aatagaaata
    19741 atattatttt caccaataat ttcaataagc tcatttgcta ttttattata tttattcatt
    19801 tttctacctc actttcaata atactgcttg aaatggtttc aaactcactg ttttatttag
    19861 tagatttata tcatctaaat tttggaatac aatatcatat ttttcttctg tttttatttc
    19921 tacaagttta tcagaaaaat tataataaca atcaataact accaaatcta attgccttct
    19981 atacgcaata acattgtcac tttctatatc aataggttct attgaaccat aaatcaaaca
    20041 atcagaatac ttactatctt gtctcaactt tatcatcttt ttataaaatt ctaaaatact
    20101 ttctgaattg ttaatctgac tctgtgcatt aatatcttta taattttcaa taacctttaa
    20161 ccatggtgtt gcatctgaaa aacctgcatt ttctttatct gcccatggaa atggcgttct
    20221 tgcattatct cggcttctta aattcacaaa ttctaacgct tcttcttctg agtaaccttc
    20281 atctattgaa cgatagtatt ggtcaatgct agataaatca ttaaaatcat ttatggtatc
    20341 tcttttaaaa tttgtcattc ctaattcctg tccctgataa ataaatggtg ttcctcttaa
    20401 aaagaaatac attgctccca acatttttac tgcatcatca ttcctttcgt attgcaaaag
    20461 atactttgtt gtagctcttg gttggtcatg attttcaata aaattagctg cccatccatg
    20521 ttcctgaaca tacatttggc tatctaaaat gcattttctt aaatctggaa ttgtccatga
    20581 aattctttta aaccactcac taccagaagc aacatctaag tcagcatgtt taaagtcaaa
    20641 gaccattgaa aagaaaccat cttctccaat aaaatcacct agttggtcat agctaatacc
    20701 tgctgcttca gcaacagtca tacaatcata ttttttaaac gtattttctt ttaattcata
    20761 aagcattttt tctattccag gctgatttct aactgttttt gtacatttaa caagaccatc
    20821 aacaccatca ctttctaaac tttgaaaact taaattcttt ttgataaatg taatagcatc
    20881 aactctaaat cctgccactc ccatttgaga ccaggtatta atcatttggt aaagttcact
    20941 tcttaatatt ggattttccc aattaaggtc aggttgtttt ttaccaaaag aatgaaaata
    21001 atattcgtct ctattctcta ccttttccca aacacttcca ccaaaaacag acctccagtt
    21061 atttggtaaa tcgtttcctt ttttaaaaat ataataatca tgataaggac tttctggatt
    21121 ttttaatgct tcttgaaacc aaggatgttc atcacttgta tgattaagaa ctaaatctaa
    21181 tataattttg attcctctat ctttactttc tttaattaac tcttttaaat cttccatact
    21241 tccaaattca cttgcaatat ctaaataatc actaatatca tacccattat cgtccatagg
    21301 tgatttaaaa attggacata accacaatga tgtaatacct aaatctttta aataatctaa
    21361 acgagaaata attccctgta aatctccaat tccatcatta ttactatctt gaaagcttct
    21421 tggataaatt tgataaacga tttctttttg ccaccatttc ttttccataa attactcctt
    21481 tttatatgat atttcttctt tttaattctt tataaattcc attatcgaat atgtcctcac
    21541 atatagatgt cgctatatct ttaagttgtt gatgactatt tttcattgca attgtaacgt
    21601 ctgatgcttg aaacatgaca atatcatttt gaccatcacc aaaacatatg gtttcttttt
    21661 gagttacccc taatctttct tgtaatctct taattgcttt gcccttatga aaatcttttt
    21721 gaataatttc ataatactga ctagaaatat ctctctgtgc taattccatt ttatcttgta
    21781 gtatatcttt cacttcgtca aatacctttt catttgacca taaacaaatt ttatgtacat
    21841 cttgtgactt atactcttca atattatttt catatgttat tttttcttgg atatgttgtt
    21901 tatttataca agaattagtt cctttcaact gattcattgt ttcaaatatt tctttggctt
    21961 tttgattcat gaaaactttt tcctgactct caatcgaaaa agcaacttct ctctttttta
    22021 acaaacaaac aacttccttt atcaatcttt gattaaaaga ttgattataa agtaattcac
    22081 catgatattg aatgtaatta cctcctcctg caatatatcc atcaacccct aatgataaaa
    22141 catcatcttg tattgtacct atacttcttc cagtacaaat aacaacactg caatgattct
    22201 tttgacataa acgtatagcg tgctttgcag attctgggat tccatatacc tcatccctaa
    22261 gcgtaccatc tatatcaagt attaataatt tgtacaattt catcttcctt tcttactatc
    22321 aaaatcataa tagcattcaa aaaaaaagat atcaatattt tgatatcttt taaaaaatat
    22381 gttcattttt agcacaaatt ttcactttcg gggaatatgt tcactttcta aatacatctt
    22441 gtatttaata taaagcagtt caattaaaat aaaataaggt gttgttacat cgtagtccag
    22501 ttgtttatct acagaaatag atatagattg aatatacagg ttttcatcac ataatcttgc
    22561 taaaggatta cttatcattt ttgtaataga aaccatataa gttcctttca ttctcatttc
    22621 tttagcaaaa tcaataattg tagatgtatc tccattcaat gagataaaga aaatcatatc
    22681 atctgttttt ataaaatgat taagcgcctg aatcatatca taaccataaa catcatatat
    22741 aattttccct gttggtaaga aaatcctttt catctcttta acaactcttt cctgagaata
    22801 accttctcca aagacgatta tacgatttgc ctttttcatt ctttcaaaca taatcgaaca
    22861 atccttcttc tctatatcat ctatcacctt attataacaa tccattaatt ggtgtttact
    22921 ttgattttta tattctactt ctgtttcatt taagcgtata ataactctta attcactata
    22981 tccaggtaat tgtagttttt gagcaaaacg agaaagtgca cttgtagata tatggtattt
    23041 gtctgcaaat tcatctatag aaagtttaat acaatcttct ttatgattaa gaatacactc
    23101 acagatatac ttatcatttg ctgtaaatga attataatat ttattgaaaa gtccttcaat
    23161 tttcattctt ttctccttct atactctatg taattcatat gaagcaaatc taatagagca
    23221 taaaatgctc ctacgacttc ataagatata gcttgtaatg tatcaagttt ttgagtttca
    23281 acatacagat tatactcaca aagtgttgca attgtattat tatcaagcct agttaaagaa
    23341 atcgtatgta tatgattgtt gataaatttt gcaatttcaa ttcctgattt tgtttctcca
    23401 gacaaagaaa tgataataaa taaatcactt ttttgaaatg attctctcat aaattcaatt
    23461 tcacctatat caaataagtc aataacacat ttacctactg aaagaaatat tctctttatt
    23521 tcttgagcta aacttttctg ttcattacct gtcccataaa tataaattgt ttctgcatca
    23581 tatattattt tacaaatatc ttgaaatgat attttttgta attcattcat taatagtcga
    23641 taattctcgc aaacatcatg aaaattatta tcattattaa caaattctag ttgattattt
    23701 ttaagtaaat actttaaatc agagaatgaa tctaatccaa tttttctaca aagccttaat
    23761 aaagtagctc tagaaacatt acattcattt gcaatttttt cacacgaaaa attacggtat
    23821 tctctttgac aaatcaatat ttgtttaata ataattttat cattttcatt taaactatta
    23881 taactttggt taactaacga ttctaatctc attttttcca ctccctaaat attattttaa
    23941 tatacattag ttgctttttc tagtgaagag tcttaaataa taaggataaa ataatcattt
    24001 gattttattt ttattaaaat aaaaataacc tgctaaaagc aggcaccaaa aagtacttat
    24061 cctttcgaac atgtacttta gatattgctt tcttcggaag cttcatctct tcatactcta
    24121 aaatagagtt ttttctaact caaaagtatt ttccatggta gaaattactt taattcatct
    24181 gccatttatt ttttatctta tttaataatt actattactt gaatatttac ttctttaagt
    24241 aaatttcata gttaacaaag ctaaaactat tattatagat gttaaaatac aaaattataa
    24301 gtattgttat aaatatgctt acagctacaa atatacatga tatattaaaa agaagctgta
    24361 aattattatc atacatacaa gcaactatgc ctctattaat ccatgttaat ttttattatt
    24421 taatacaagg agttccatga gacatacata tccattcata ttttaaatta tccaattctc
    24481 ttatagattt catcatcttt ttataatccc aattccatgg attaggataa ggaactatat
    24541 ttccattctt atttttgact aaatcacctg caaataatac atcttcaaaa atcatgcaga
    24601 catgccctgg agtatgacca ggagtatgga taatttttat attcccaatc ttcatattct
    24661 ctccataagg ttcaacatcc ttaatgagcc ttctggaaat tgcctttcca ataaacttct
    24721 taaacccagg tctatccttc tctcctataa taaaaggtat atcctctaca tgagcccata
    24781 cctttgctcc tgttttctct ttcaataatt taatattaga tatatgatca acatcatgat
    24841 gagttaataa aatatgctta atatctgtaa gtttaatgcc aagtgttttt aattctttaa
    24901 ttatattttt ccccataaaa ggaagacctg tgtctataag aataatttct ttatcgaaca
    24961 caataaaaca atttgaacca cgagtactat ctaacattaa tacattttct tttatcttca
    25021 taaataaatt ccccttattt ggtattttac ctatataatt aattaataaa gttataaata
    25081 gttataatac ttgatattaa ttttattttt tattaaagta tactataggt atacaaatat
    25141 tatatttaca taagtatacc atcagtatac ttttttatca atagaaagga ataatttata
    25201 tgtcagaatt atcaaaaaga gataaagaaa aaatacaaag agaaaatgag attattgata
    25261 aggcagaaaa attattttgt cttaatggat ttgattaaaa aatagctacg cacactcgtg
    25321 actttagtca tgagttagta gctaaaatag tcagcatata gggaaaccta tatgtagaga
    25381 taggataa
//
LOCUS       DAMJVX010000039        25048 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMJVX010000039 DAMJVX010000000
VERSION     DAMJVX010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25048
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.041"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            29..1885
                     /locus_tag="PC407_001497"
     CDS             29..1885
                     /locus_tag="PC407_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2496949.1"
                     /translation="MDIHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYRGGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIIDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALINKIFESDKGVVENKKVVTFELNVSINDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSENNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    1968..2208
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2274..2509
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2594..3811
                     /locus_tag="PC407_001498"
     CDS             2594..3811
                     /locus_tag="PC407_001498"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2496950.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRSIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            3925..4419
                     /gene="cdeM"
                     /locus_tag="PC407_001499"
     CDS             3925..4419
                     /gene="cdeM"
                     /locus_tag="PC407_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HDF2496951.1"
                     /translation="MENKKYANGGYSEDWYERGESTAKWFQNDREEYERAAYDEDRER
                     RGSNCGCSDSGGNRPRNCERFRREAEIREREAREAFCESSERKKEALAYECEARKLWE
                     EAEKYWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCN
                     CCRK"
     gene            4684..5949
                     /gene="hisD"
                     /locus_tag="PC407_001500"
     CDS             4684..5949
                     /gene="hisD"
                     /locus_tag="PC407_001500"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDF2496952.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6152..6904)
                     /locus_tag="PC407_001501"
     CDS             complement(6152..6904)
                     /locus_tag="PC407_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2496953.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANKKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(7022..7291)
                     /locus_tag="PC407_001502"
     CDS             complement(7022..7291)
                     /locus_tag="PC407_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496954.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            7766..8860
                     /locus_tag="PC407_001503"
     CDS             7766..8860
                     /locus_tag="PC407_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2496955.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LRERSINGVISMCLKETDYQYSLSNKKGGY"
     gene            8862..9812
                     /locus_tag="PC407_001504"
     CDS             8862..9812
                     /locus_tag="PC407_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDF2496956.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGLKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKIDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            9805..10989
                     /locus_tag="PC407_001505"
     CDS             9805..10989
                     /locus_tag="PC407_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496957.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKKSLMREMNNVSKGVIDNEDTPSGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKSLLEISSYFKSKGLKIGIDTFSPFISYFTGQDLKKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIIAPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            11002..12498
                     /locus_tag="PC407_001506"
     CDS             11002..12498
                     /locus_tag="PC407_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2496958.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNSHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHTVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSEGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            12521..13471
                     /locus_tag="PC407_001507"
     CDS             12521..13471
                     /locus_tag="PC407_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2496959.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLVSFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            13492..14454
                     /locus_tag="PC407_001508"
     CDS             13492..14454
                     /locus_tag="PC407_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2496960.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            14826..15458
                     /locus_tag="PC407_001509"
     CDS             14826..15458
                     /locus_tag="PC407_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2496961.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            15869..15961
                     /gene="kdpF"
                     /locus_tag="PC407_001510"
     CDS             15869..15961
                     /gene="kdpF"
                     /locus_tag="PC407_001510"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDF2496962.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            16002..17684
                     /gene="kdpA"
                     /locus_tag="PC407_001511"
     CDS             16002..17684
                     /gene="kdpA"
                     /locus_tag="PC407_001511"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDF2496963.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            17709..19766
                     /gene="kdpB"
                     /locus_tag="PC407_001512"
     CDS             17709..19766
                     /gene="kdpB"
                     /locus_tag="PC407_001512"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDF2496964.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNVGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMIL
                     NLG"
     gene            19785..20411
                     /locus_tag="PC407_001513"
     CDS             19785..20411
                     /locus_tag="PC407_001513"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDF2496965.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNASYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNIEVAKILGLI"
     gene            20648..21556
                     /gene="cysK"
                     /locus_tag="PC407_001514"
     CDS             20648..21556
                     /gene="cysK"
                     /locus_tag="PC407_001514"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDF2496966.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            21583..22173
                     /gene="cysE"
                     /locus_tag="PC407_001515"
     CDS             21583..22173
                     /gene="cysE"
                     /locus_tag="PC407_001515"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDF2496967.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            22333..22521
                     /locus_tag="PC407_001516"
     CDS             22333..22521
                     /locus_tag="PC407_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2496968.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(22575..23444)
                     /locus_tag="PC407_001517"
     CDS             complement(22575..23444)
                     /locus_tag="PC407_001517"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HDF2496969.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDMNKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(23641..24885)
                     /locus_tag="PC407_001518"
     CDS             complement(23641..24885)
                     /locus_tag="PC407_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496970.1"
                     /translation="MENNLNIAKKNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYYMENNTNITIYENNTLQYKFDEESEDIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKSRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
BASE COUNT         9636 a         2681 c         4511 g         8220 t
ORIGIN      
        1 aataacattg aaaggtagtg gttagaatat ggatatccat aataaatatg taaattttat
       61 aaaaaatata cctgtacctt ttttatattg tagaattgta aagagacaag aagatataga
      121 atatcgagta gaatatataa gtaagggtat gggcaaagta ttacaattag aagaaggtat
      181 ttgtgataaa aatatactag atgtattgcc tgtatttaag tctaaaaaat atttcaaaga
      241 gttgttctca aatgaagtgg attgtataaa aaggtacatt ccgacactta aaaactggat
      301 aaatataaaa aaacagataa taggggattc atacataatt ttgtattttg gtaaaattgt
      361 atttgattat agacagataa tcgattcctt tgataaaaag gaaaaagtag cttatattaa
      421 agacgaagaa ggcatatata tagattgcag tgaaaattta ataccaatat taaataacaa
      481 cattaaaaca acgaaagata tctttggtaa aaatgatata gaagtatggg gagagaatac
      541 aggaaaatta tttagagatg attatagagg aggagtatct agtaaaaaaa gatttttaca
      601 aaatcttttt gaatatgaag aaacattttt tatggttgaa aaatattttt tgtatgatga
      661 agacgaactt ttaggtacta tagggataat agataatata atatatagtg gttacagtaa
      721 tagaaattat aactcaaaag atttaatgaa gatgatagaa cactcaattc cagagaatat
      781 gttttataaa gatgtgtatg gaaattatat tggatttaat tcaggttttt tgaatttagc
      841 ttgtatgaat aaagaagaat tattagggaa gaatagttat aaaatatcag aagaagaagc
      901 tttaataaat aagatatttg aaagtgataa gggtgtagtt gaaaataaaa aagtagttac
      961 atttgagtta aatgtaagta taaatgatga aaataagtgt attgagatta caaaaaggcc
     1021 gttttttgat agctatggaa gtgttattgg aataattgga acagcaaggg atataagtag
     1081 aagaaaaaga ttagaagagg aaatggataa aactaggatg gaattttttg caaatttatc
     1141 ccatgaactt agaacgccta taaatttaat atcatcttca ttacaagtga tagaaaaaaa
     1201 agaagcagat ttaatagaat caaatgatac tttaaaaaga aatttaggta taataaaaca
     1261 aaatggaaat agaatactga ggttagtaaa taatgttata gactttacaa aaatgcaatc
     1321 aggatattta gattttaaac cagaagaatc tgatataatt gcatttatag aggagatatg
     1381 tatgtctgta gctgattttg caagccaaaa taatatacaa cttacatttg atacagaaat
     1441 agaagaattt tcaatgttat ttgattcaga aaaattggaa agaataatat taaatttact
     1501 ttctaatgga attaagtaca ataagaaaga tggaaaaata aatatatttt tatatgtgaa
     1561 agataatgta tttaatatga aaatatctga tagtggaata ggtataccaa aagaaaaaat
     1621 agataaaata tttaatagat ttgaacagat tgacaatgaa ctttcctaca gagttaaagg
     1681 aagtggtatt ggactttcat tagtgaaatc tttggttgaa ttgcatgagg gaagtatatc
     1741 tttaaaaagt caacttggta ttggaagtga atttatagtt tcattacctg ttagaagtga
     1801 aaataatatt gaaaaatata atcataaaag agaaatcagc aatgaactaa gtaaaaaatt
     1861 agaaatagag ttttcggatt tataagttta aatataaaaa ataaaatttt aaaaaagtat
     1921 tgacaattga tttagaaggg tttaaaataa attacaacat aataaataaa aaggcgatga
     1981 ataggaagag taaatctaat tcgattacca gagagaagag tccattggct ggaagacttt
     2041 ttaaattaga gggattgaaa actacctagg agctactttc tgaagtcatc tgcattagta
     2101 gattttagta agaatagtcg ttagctgcgt taaagtgtaa agtgagtttt catgatatat
     2161 gaaaactaaa ttaggtggaa ccgcgggaat aatactctcg tccttatgtt ataaggatga
     2221 gagttttttg ttgtttaaaa atataaacag taatcataat aattatagaa aatataaaag
     2281 cgatgaatag gaagagtaaa tttaattcga ctatcagaga ggaaagttca caggctgaaa
     2341 gactttctaa gtttgagaaa ttgaaaacta cctaggagct gtggtctgaa gttactttaa
     2401 aaagttttag taagactaac cgttaactgc gttaaagtgt aaagtggatt ttatcaataa
     2461 atgataaaat caaattgggt ggaaccgcga gtatagactc gtccctttat atagggatga
     2521 gtctattttt attgcaataa aagattgaaa ataaaaaaaa atcggaaaat tataaattta
     2581 ggaggaaaat aaaatggaaa ataaagtgaa aattggagta ctaggatatg gagttgtagg
     2641 aagtggactt atagatataa tagataataa taaagaaaaa agaagtatag aaatagttgg
     2701 aattttagtt aataatttgg aaaaacataa ggataaaaaa tattctaata taataactaa
     2761 taatatagat gatatattta ataaagatat agatatactt gttgaagtca tgggtggttt
     2821 agaaccatca ttatcttata taaaaaaagc attaaacaat aaaattcatg ttgttacagc
     2881 aaataaagat ttgttagctg aatgtggaga tgagcttgca aaattagcaa gtgaaaataa
     2941 agtaagtatt aaatttgaag cttcagtcgc aggaggtatt ccagtattaa aaccaataat
     3001 agaatcacta gaaggaaata atatagatag tataaatgca attttaaatg gaactactaa
     3061 ttttatatta tctaaaatgt atgatgagaa tctatcttat gatatggcat taagacaagc
     3121 tcaagaatta ggttttgctg aagcaaatcc agagtcagat gtattagggt atgatgctgc
     3181 tagaaaacta tcaatattat caactctagc ttatgacaat agagtatatt ggaaggatgt
     3241 gtatttagaa ggaattacag atatagatga aaaagatatt gaatatgcaa aaaaattaaa
     3301 ttgtaaaata aaattgattg gtcaaagtaa gtatgaaaat gataaagtaa gtgcttttgt
     3361 tagacctgtt ttagtagaaa aagataatat ccttgctaga atagacaatg agttcaatgc
     3421 tgtcatagtg aatggagatt cagtaggaga agtttctttt gtaggtaaag gagctggaag
     3481 tttagcaaca ggaagtgcgg tttattcaga tgttatagat ataatagata atagagtttc
     3541 aagtatagat tcttttacta aagataaaat acaagttaat aaaatagtta gagaaaagtg
     3601 tggagcactt cttagattta aaaaatgtaa taaagatgaa attctaaata tagttgagaa
     3661 ttgtttagta aaattcgata ttttaaatga tgatgatgaa cttgctatta tggtttatgc
     3721 tgattctgaa tatgaaataa acaacagttt atgtctaata aaagataaag gatattgcga
     3781 aaaaatgaat aaaatgttga aaataagtta aaaaagaaaa aataagttta tatgtaacat
     3841 attttgacga ttttaatact atatagtatg agatgaaaca aaacatctca taaaaaatta
     3901 aaattcaagg gagagatatg ttatatggaa aataaaaaat atgcaaatgg tggttattca
     3961 gaagactggt atgaaagagg agaatctaca gctaaatggt tccagaatga tagagaagaa
     4021 tatgaaagag cagcatacga tgaagataga gaaagaagag gctcaaactg tggatgttca
     4081 gattcaggag gaaatagacc tagaaactgt gaaagattta gaagagaagc tgagataaga
     4141 gaaagagaag caagagaagc attctgtgaa tcttcagaga gaaagaaaga agcactagca
     4201 tatgaatgtg aagctagaaa attatgggaa gaagcagaaa aatactggga tgaatattca
     4261 aaatacaact ataaaggaat cgaatattta gcagaagctg ctagattatt tgatgaaggt
     4321 atggaatgtg aagctagaag aaatggaaat aatggaggaa acaataataa ttgttgccat
     4381 aaatgccata aatgtaattg taactgctgt agaaaataaa aaaattcatt gacaaatcaa
     4441 tagttaaaat atatcatatt atgtataata aagagaaaat tcagaaaaaa ataatgatta
     4501 gaataaattt aagtatattt attagacaaa tagtgctcag ttagtgaggt gagtacaata
     4561 tgattaaact cccatcctct agcttttgat agagggtggg agtttttatt tattgatata
     4621 tcttaaatga ggataatatt gtaaaataaa tgagtatgag attgtaaaat taggaggtaa
     4681 aaaatgaaaa gtattaaaac atcaagtatg aaagaaaagg tagatgaaaa gatattaaga
     4741 gagcaagtaa gttctataat agaagatata agaaataata aggatgttgc acttaaaaaa
     4801 tataatgaga agtttgatag aaatacaaga gatgaattta gaattactaa ggaagaaata
     4861 aaggaagctt ataagcatgt agatgacgaa tttataaata acttaaagat tgctgccaaa
     4921 aatataagag aatttgctaa agctcaaaaa ctaagttttg aaaacccttt tgaaaaagaa
     4981 atttatccag gtgtaatctt aggtcaaact aatattccaa tagagtcatg tcttgcatat
     5041 gttccaggag gtcagtatcc attgttttca acagctctta tgcttataat acctgcaaaa
     5101 gtagcaggag taaaaagaat agtggcatgt tcaccaacta tgaaaaatac tgaaaagata
     5161 aatcctaaaa cattggtagc tatggatata gcaggtgctg atgaaatata tgcaactgga
     5221 ggagttcaag ctatagcagc atttacatat ggaacagaaa agattaatcc agttgacata
     5281 attgtaggac caggaaataa gtttgtaact gaagctaaaa gacaatgtta tggacaagta
     5341 ggaattgatt ttgtcgctgg gccaagtgaa gttcttataa tagcggatga aacttcaaat
     5401 ccagtttata ttgcagctga tttattagca caatgtgagc atgatttaaa tgcaagagga
     5461 atattattga caaatagctt ggaagtagca caaaaagttg aaaaaaatat agaaagtatg
     5521 ctaaaagatt tgccaacaaa ggacatagca tatagttcat gggaaaataa tggagagata
     5581 atattggttg atgatatgga agaagccatt aaaataagta atttttatgc accagaacat
     5641 ttagaagttg cagtaaatga gtataatgat atttgtgata gattaactaa ctatggttct
     5701 ttatttattg gaaatctttc tgctgaggtg tttggtgact atgtttcagg taccaatcat
     5761 acattaccaa cacttaaagc atcaagatat actggtggtg tttgggttgg aacttttatc
     5821 aaaacttgta caaagcagat atttaatgaa aaagctatac aatcattagc acctgtagct
     5881 gaaaagctgg caaaagaaga gggattatat gcacatgcaa aggctgctga ggttaggttt
     5941 aaaaagtagt aaaaaatttt attttggcag tcgagattta gtattagact gagataaaga
     6001 aaacgctgag aacctgaata aatactatat tttgtgggcc tcggcgtttc ttttatatct
     6061 gtatataata ggataatact acctgtgtgt ttaaatgcta acttaatatt tcttttctaa
     6121 ataatgttta tgaaatagtt aatattttta gttaatactt gaaaaaaact tttgctaact
     6181 gtgcaaattc atctgcattt gtcaatatag cagggtgtcc tccctgacta aaaatgtgaa
     6241 tacttgcttt tgaaataaaa gttgccattt gcttgtactc ttcttcaaga ttatgacgac
     6301 acatttcgtc ttccttactt cctgtaaata aaattggcat ttctaattta cacaattctt
     6361 tatgaaataa agggcgtttc ttgtttgcac attgcaatag agcttttgta tcaagattaa
     6421 caacattctc ccaatctttt ccatgacacc attcatagaa ttttcgtgct tgaatatctt
     6481 gctttgattt ttcccttcca gtgattagat tatctatgaa gttatcatta agagtacgtc
     6541 catcaaagct atctgctata attgcttcaa ctaaatcggg acgttctaat ccagtattaa
     6601 cagctgccca tgctcctcca cttgttccaa ttaagccaac tttaccacaa ttcaaatgtt
     6661 caatcagtgc tattgtctgt aaggcttcat catgccatat gtcaggagaa aattgagaaa
     6721 ctctatcaga ctgtccatta cctaaaaaat caattaagat acatctaaaa ttttctacat
     6781 ataatggcac aagtgcttta aacatattag aagaaccagt attaccatgc aggaaaatta
     6841 tcggtttacc ttgtccatat tctttataaa aacaagaatt accttgatac tgaaaatatg
     6901 acatatattt tcctcctttt tatttgttaa gttaattata acaatatagg tatgaatgaa
     6961 aaacctcatg ttctttctta gaataaaaat gtgccacgca taaaacgtga cacatttcta
     7021 attataaatt ttgctgttta agtattcttt gtatgctttt ctctgtcaaa aagtatttct
     7081 ctgataactg cttgatactc attccatttt ggaaatttat acagatttga gaattacgag
     7141 aagctaagac ttgtttggtg tttgtgtttt caccccaatg ctttttgtgc tcttgctttt
     7201 tgggaatata aatcacttta ccatctatat attcttgcac ttgctcaatc agttctttag
     7261 gcagtaagtc aatcgaattt tgatagctca ttttgcacct cctaaataat tactctttag
     7321 gattagcaaa ggctactgct actcacaatt aaaaataaaa ttttttatta tgtacaatag
     7381 cctttgctat gcacaagttc caattcccat aaactaaata ctcctttctg gtttgaaaaa
     7441 ttcatctgaa cattgatttc gattttaaga tagagttcaa tgacaaattt acatttttat
     7501 tatatatatt cagatagata attgcaactg tctttgtatg aggatatttt attatagtca
     7561 atactataat atatatttat atcatcaaac taatatgaac agttgaaagg aatatgaaca
     7621 ataaaactta gtcaggaaga ataattatga agcagacaat tgagaaattt gattttaaag
     7681 ttttattgaa aatttattga caaatttaac ttgtaaatat tatactaaac ttaataaata
     7741 aagtttataa agtaaggtgt tgtctatgaa aaatatagtt ggaaaaccgc aaacgataaa
     7801 aaaagtaaat gaagatttaa taaaaaacat tataaaaaat gaaggtccta taacaaaacc
     7861 agaaatttca agcattactg aattgagttt agctacagtt aataagattg tggagcaatt
     7921 agctttgaaa aatgaagtta aagtaagtgg actaagtgaa tctactggag gaagaagagc
     7981 tcaattattt gaaataaatg caaacttaga acacataata gctttatatt attatagaaa
     8041 ttgttgtata ggggtagttg ccaatttatt aggtgaaata atttatcaag aagaatttaa
     8101 tataagaatg gatacatatg aaaacgttaa ccaagatact ttttttgtta tagatactct
     8161 aataaaaaag gaaaaaggaa acaatataag ggctattgga attggtgtcc caggagttgt
     8221 aaaaaaaggt gtagttaaaa atataccaac tataaaaagt tgggaaggta taaatttaga
     8281 aaatttaata aagagtaagt acaatactaa agtgtttata gaaaatgata caaatattac
     8341 tactattggg atttatcaaa aacagtataa agacaaatat aaaaatatgg ctcttatgta
     8401 tttagaagat ggtattggtt caggtatagt tataaatggt gaattgtatt taggttctac
     8461 aaattttgct ggagaattaa gttatctaaa tatagaattt ggtaagtata actctaacaa
     8521 cttaaatctt gaagattata ttatgagttt aagaaaaaga tatttagaaa gtaaagataa
     8581 taaatttaag aaagaaattt tatctataat atcaaacttt ataaggactc ttgtatgtgt
     8641 cctaaatcca caagctatga ttatacagtg tagtatatta accaagagtg acatggagtt
     8701 tatagaaaaa gatgtaagta ataatgttgg agaagaaaat tttcctaaat taataaaagt
     8761 agagtcattg agagaaagaa gtataaatgg agtaattagt atgtgtttaa aagaaactga
     8821 ctatcaatat tccttatcta ataaaaaagg aggatattaa tgtgaaatat ttggtttcaa
     8881 tagatggagg gggaacaaaa actaaatttt gtgtaagtga cttagatgga aacatactta
     8941 aagaacatac cactggttca actaattata agtcagttgg aattaaaaaa acatatgaaa
     9001 atattaataa tggacttaaa aaaatattaa aagatttata tatcgattat gacgatattg
     9061 agtacactgt atttggtata tctggttgtg attctcctaa tgattataag attataatgg
     9121 atgaaatatt aaaaattggg attaacaaag aaaaaatata tctggcaaat gatgcagttt
     9181 tagcttttta tgcacaagct gattcccctg gtttagtaat tgtggctggg acaggttcaa
     9241 ttatactggg aattaaagaa gatggagaaa tatatagagt tggtggatgg gggtataatt
     9301 ttagtgattt gggttctgga tatgatatag ggagaaaatt attaaagaaa gtattgcttt
     9361 attgtgatga atgtcatgag tatagtgatt tatttagttg tgtcttggat ttctttggag
     9421 caaatagttt tgaacaactt gcatatatga ttactgatat aaacaataat gttgaaatag
     9481 caaacctagc ttcattagtg attgattgtg ccgaacaagg tgataaatta gctattgaaa
     9541 tattaagaga atcatcaact gaactatcta aattagctca agtaatattg agaaaaatat
     9601 ctaatccaat gaaaatagat aaaagcgaaa taaatatagt tttgtctggt ggtacattaa
     9661 ataaaactgt atatgctcaa atgttaatga ataatttaaa aacagaaaat acagataaaa
     9721 atttaaaatt tatatctcaa gaaaatcagc cagtatatgg ggggataaga cttgctatgg
     9781 cattagctaa taggagagtt aagcatggat agaaaaaagg taatagtaca aatttttact
     9841 ggaggtttta aggctaaaga aataaagttt aatgatataa aatgtaaact actagaatta
     9901 ttggactgta ttgatataga aaaggttatc atgggatgga gtgtagatga agaattatat
     9961 gtagaaacta aaaaactatt aaataaatat aatgttgaac tatatctttg gttgccatgt
    10021 ttttctgaaa taggattact tgaaaaaagt aatttactaa ttgattatat ggggaaggaa
    10081 gtcaagggct atgctttaca agaggatgaa aattttgaat tttattgccc aaataataag
    10141 gaaaatataa aaaatataaa gaaaatatat agtacttatt ttagtgatat taaatttgat
    10201 ggaatttttt tagataaaat aaggtatggt tctttttcga ataaactaaa tggtgttttt
    10261 aattgttttt gtaaagattg tttaaatata tatgaaaaaa ataatattga taaaaagtcg
    10321 ttaatgagag aaatgaataa tgtgtctaag ggagttatag ataatgaaga tactccatct
    10381 ggtataacag aatacattaa tggaaaatac aaatttaaaa ataaaatatg ggaagatttt
    10441 tttgaaacaa aaaatcaaaa tatatacaag tcattattag agataagtag ttattttaaa
    10501 agtaagggat tgaaaatagg tatagatact ttttctcctt ttatttctta ctttacaggt
    10561 caagatttaa aaaaattaca agaagtagca gattttataa aaccaatgat gtatagaact
    10621 acaaaagctc cagctggttt accatttgaa tttgaaaggt ttattgatga atccatacat
    10681 aagaataaag agattggaaa aagaaatttc ttagatattt taggaattaa agagtatgaa
    10741 ggagatgtat atcctataga atttgtacta gaggaaatcg attttatgac aaggaatctt
    10801 aataaagata tatatgtagg agttgaaata aataaaaagg aaattatagc tcctgttaca
    10861 cctaagtaca taagtgaaaa tatttcaaat ataaataaga caacagtaaa aggatatgtt
    10921 ttatcttggg atttgttatc agcaccaaaa gaaaatattg atgaaattat aaaatggttt
    10981 cggaattagt gaggtgatat agtgagtaag atattagaaa tgcaacaaat atataaaagt
    11041 tttggaaagg tagatgtttt aaaagggata aattttgagt tagaaaaggg agaagtaaga
    11101 gctcttttgg gagctaatgg agcaggaaaa tctacactta ttaaaatttt aggaggagtt
    11161 cattctcaaa ctaaaggtaa agtaattttg actggtaatg aaataatgtt taaaaattca
    11221 catcattcta aagaaaatgg tattagtata atttatcaag aattaagctt gataccaacg
    11281 cttacagtac ttgaaaatat ttttcttggt agagaaattt taaaaggaag tgtgttttta
    11341 aacaagaaag acatgcttga ggaatatatg aaaatttgtg aggattttga ttttaatata
    11401 gacccacata cagtagtttc taatttaagt gtagcaaacc aacaaatggt tgagataatg
    11461 aaagctgttt catgtaattc tgaaattatc ataatggatg aacctacaac atcacttact
    11521 aataatgaaa aagatgggct atttaaaata ataagtagac taaagtctaa aggtaaaaca
    11581 atagtctata tttctcacat attagaggaa gtttttaaag tatgtgatag tgcaagtata
    11641 atgaaaaatg gaattatgat tggaacttat gatgtagagg atttaactaa attaaagata
    11701 actcaactta tgacaggaaa tgataatcac aaacttattg agaagaagga ttatacattt
    11761 gcaaagtatg aagagccacc agtactagaa gtaaagaata taagctggtc taacaaaata
    11821 aaaaatgttt catttaaagt ttatagaggc gaagttgtag gtcttgctgg tcttgtaggt
    11881 tcaagaagaa ctgaaattat aaatttgata tttggtatag ataataaaga tagtggagac
    11941 atttttatca atggtaaaaa agtagacata acttctccta aggttgctat aaaaaacaaa
    12001 ataggattga ttcctgaaga tagaaaaaat ctaggattaa ttttagaaca agctatatac
    12061 acaaactcat catcagtaca aattgataaa ttcaagaaat ttttgtttat agatagaaat
    12121 aaagaaattg agttttctaa aaaaggtgtt aaggaattag gaataaaagt tagtgatgta
    12181 aagcaagaag taaaagagct tagtggtgga aatcaacaaa aagtagtagt ttctaagtgg
    12241 ttagagaaag atttagattt aataatatat gatgagccga caaagggaat agatattgct
    12301 gcaaaagaag atattttcaa tacaatcaaa gaattttcaa gtgaaggaat aggagtaata
    12361 tttatatctt ctgatttaga agaagtaata agagtaagtg atagaatttt agtaactaga
    12421 gaaggttcta ttgtagatga tttaaaaaat gagaatatac aggttcaaga tataatgaac
    12481 agtatattta atgtataaaa aatgaaaatg gaggactgag atgaagaaag cttttgagaa
    12541 gttaaattta agagattatg gagtattatt aggtttttta gctttatgta tagctataag
    12601 tattgcaact ccatcatttt taggtaaaca aaatatactt aatcttttac gacaatcttc
    12661 tataattgga ataatttcag caggtatgac ttttgttata atatctggaa actttgatat
    12721 atcagttgga gcagtagcag ctcttgcagg tgctataaca atgaaatttg cgactggtgg
    12781 aactaatttg tttgcatcca tgctcttagg tgtagcgatt tgtgcagtaa taggattaat
    12841 caatggtata gtagttgcaa agataaatgt accatcatta atagcaacta tggctatggt
    12901 cactattgta agaggattgc tgttgatgct tacaggagga tatccaatta ctgaaaatat
    12961 accaatactt gattacattg gaaatggata tttgtttaat ataccaatac cagtagtaat
    13021 tttctttata gtggtgttag tttcatttat tgtacttaat aaaactaagt ttggtagata
    13081 tgtttattct gtaggaggga atcaagaagc aagtaaactt aatggtataa atgtagattc
    13141 tcacaaagtg aaagtgttta ttattaatgc tgtacttgca ggaatagctg gaatcgtact
    13201 aacaggaaga cttgggactg caactgctat tgcaggtgaa ggatatgata tggatgcaat
    13261 tgcatctgta gtaataggag gtacatcagt ggcaggaggt tctggttctg tacttaaaac
    13321 tgttatagga gtattactta tgagtgttat aaacaacagt tttaatcttc ttggaataga
    13381 tgtatatttc cagtacatat ttaaaggatt aataatttta gcagcagttg gatttgattc
    13441 ttatagcaag aaaaaacttg cttcaagata aaaaataagg gggatttttt tatgaaaaaa
    13501 ttaatgaaac atttagcatt gttgttatca cttgttatga tatttggttt agttggttgt
    13561 tcaaatggtg gtgacaaaga aaaaaatgga gaaaagaaaa tagcagtatt actaccaggt
    13621 tcaacaggtt actttgtagc aactaaacaa ggtatagatg caaaatctaa agaattaggt
    13681 gtaagcgtgg aatatgcaga tgctcaatgg gatgctagta aacaattatc acaagctgaa
    13741 gattttatgg ctaaaggggt agacatgatt gcattatgtg gagttgattc tgctgtaagt
    13801 gagagaatag ttaaagctgc taatgattca gacgtcccta tagtagcatt tacaaattct
    13861 ataggaagta acccaactgg agaatttaaa ggtttagtta catatatagg acaaaatgag
    13921 gaggaaacag gagcactaac tggaaaaatt gctaaaaact tattaggaga aactggtggg
    13981 aaagctgtat taatagaagg agtaccagga actactccac aagtattaag aaaaaaaggt
    14041 cttgaaaaag aattaaaaga tagtaatata gaaatagttt ataatcaaac ttcaaattgg
    14101 gagaaagagc aagctttaaa agtaacagaa gatttaattc aaaagaaaac tgattttaat
    14161 gttataatat gtcaagatga taattctgct acaggtgcag gtcaagcttt aaaagatgct
    14221 ggtcttaagg ataaagttaa agtcataggt ttgggtggaa gtaaagatgg attaaaagct
    14281 ataactgatg ggttaataga tggtacaact tatatgtctg ctgtagaaga aggtggatta
    14341 gtcatagaaa aggcatctaa attccttaaa ggtgaaaaaa tagaacctgt tacacaaatt
    14401 aaacaagttg aagttaacaa agataatatt tcagagttca agggtgaatg gtaaattgtc
    14461 atagataata aataagattt ttagagttac tttagaaact atataaagta actctttttt
    14521 taatttaatt tagtgataaa ttattgaata gtatttctaa acaaataaaa aattaatata
    14581 aacaaagact taattatact aaattataag taaaactaat ataaacttat ggaaaaaagg
    14641 aaaacgtcta aagtattgga attactatag tgtaggtgtg gtattaaaaa aacataaaaa
    14701 aacataaaaa aattgttgaa aaaaatatag aaattttgta taattaaaga tttgaaaagt
    14761 tcggtataca aagccaattc ttttgtatat aatatatata aattcagaaa aaggaaggga
    14821 tatgtatggc caggcggtta tttttagaaa ggattggact tatattttta ggttcagcaa
    14881 tattagcatt tggagtttat aatttttatt atttaaacaa cataacagaa ggtggagtgt
    14941 taggtatttt actactgtta aaaaatttat tcaatataca accagcaata gcaaatgttg
    15001 taatagatgg tttactactt ttagtaggat ataaattttt cggtaaaaaa ttccttatat
    15061 attcaatagt agcttctata actttttcag tgttatatga cttatttgaa gctataggac
    15121 ctctagtacc acaatcacaa aatatgcttt tatcaactat tttggcaggt gtaactgttg
    15181 gaactggtgt aggtatagta gtaaaagcag gttgtgcatc aggaggagac gatgctttag
    15241 cattagtaat ttctaagact acatctttga acatagggca agtttattta gcaacagatg
    15301 ttattgtatt gttgttatca ttattttatc tatcagcgtt tgatatcttt tattctttga
    15361 ttgctgttac aattagtggc aaggtaattg attttattta ttatcatggt aaaagtttgg
    15421 atatggatat aagtaatgat atagttccag aatgttaata taaattaaat aactttagat
    15481 tttattaggg gtggcagatt tgccaccttt tttgtgtcta aatctttata gaaagtataa
    15541 tcccatctag gtttttgcaa atctcaaata tagaattatg ttggaatgac tgggaatacg
    15601 attgctagat gtgcatttta aaaacccaat tttatattta tgaaatacta atcattaatg
    15661 gatgtttctt aatcctatct ttatacctaa aatgataaga taaatgaagt agcaatacaa
    15721 aactaaaatt tgtaaaagag attaaattaa atggaggagc taaaatgttg gatatagtta
    15781 tgatagcatt gttattcata ggatttgttt ccatgaagtt acttgctgat tggtgtggga
    15841 aacaaactga gaaaaaataa tgtaatgtat ggagggatta cctatgtggt ttctggcagt
    15901 tgtaattata atgttaataa tatatttggt atatgcgctt attaatcctg aaaaatttta
    15961 aacagtataa ggctaataag taagttgaat tggggggaac aatttttatg gtgcagatag
    16021 taatagtgtt agcgattttt atgattttag taatacctat gggcaagtat ttatatcata
    16081 tagctactaa tcaaaaaaca tttggagata gactatttga taaagtggac aattttatat
    16141 ataaggtatg tagtatagat aagaaaaaag aaatgaactg gaaacagtat gcattagctt
    16201 tattatttac aaatgcagtt atggtattta taggatatat catacttaga actcaaagta
    16261 tgcatatttt taatccaagt ggaattaaaa gtatggaaca aggactatca tttaatacaa
    16321 taataagttt tatgacaaat acaaatcttc aacattattc aggtgaatca ggactttcat
    16381 actttagtca gatgacagtg ataatatata tgatgtttac atcagcagca acaggatatg
    16441 cggctgctat ggcatttgta agaggtctag tcggtaaaaa gaaaacattg ggaaattttt
    16501 atgtagattt aattaggata acaacaagag tacttttacc aggagcttta attattgggc
    16561 ttatattagt tacacaagga gtaccacaaa cttttgctgg gacagaaaca gtgactacta
    16621 tagaaggaaa gcttcaagat atagctagag gtcctgtggc agctcttgaa tcaataaaac
    16681 atttaggaac aaatggtggt ggattctttg gttcaaattc atcacaccca tttgaaaatc
    16741 caacaataat atcaaatata gtagaaatat tatctatgat gatattacca ggagcttgtg
    16801 ttgttgcatt cggtcacatg ataaagaata aaaaacaagg ttgggttgtg tttggcgcaa
    16861 tgtcaatcat atttttaata ggtctagtag tgtgctttaa agcagaaagt gcaggaaacc
    16921 ctattttatc acaattaggg cttaatcaaa gtatgggaag tatggaaggt aaggaagtta
    16981 gatttggtat agcacaatca tcattattca caacagtgac tacttccttt acgacaggta
    17041 cagttaataa tatgcatgat acactgactc ctttaggagg attagtacca cttttaaata
    17101 tgatgctaaa tgtagtattt ggtggtaaag gcgtaggtct gatgaatatg ttgatgtacg
    17161 ctataatagc agtattctta tgtggattaa tggtaggaag aacaccagag tttttaacta
    17221 agaaaatcga aggaaaagaa atgaagctta ttgctttact tattatatta catccacttt
    17281 taatactaat gttctctggt ctttcagtag caataccagc agggcttgaa ggaatttcaa
    17341 atcctggatt ccatggatta tcacaggtat tgtatgagtt tgcatcttca gcagccaata
    17401 atggttcagg atttgaaggt ctaggagata acactatgtt ttggaatata acaacaggta
    17461 tagttatgtt ctttggaaga tatgtgtcta ttatagtact actagcaata tcaagtttgt
    17521 tagcatctaa aaaagctgta aatgaaagta taggaacact tagaacggat aattttacat
    17581 ttacaatagt tttagtactt gttgttttaa ttgttggtgc tttaacattt ttcccagcct
    17641 tagcgcttgg acctatttcg gagcatctag tgttgtggca ctaaaaaatt gggagggagt
    17701 aaagttttat ggaaactaaa aaaacaaagt ttattacaaa agatattttc aaaacttcca
    17761 ttatagaagc ttttaaaaaa ttaaatccta aatatatgat gaaaaatcca gttatgtttg
    17821 ttgtagaaat tggatttata gtaacattga tacttacaat agtacctagt ttgtttggtg
    17881 atgaaggcaa taatttaaga gcttataatg ggattgtaac aataatctta tttataacag
    17941 tcctttttgc aaattttgct gaagctgtag ctgagggaag aggtaaagct caagcagata
    18001 cacttaagaa aactagaaaa gatacagtag caagggttat agatacgtta ggaaatgaaa
    18061 aaatgataag tgcaagtgag ctaaaacttg gtgatattgt tttagtaaat gttggagaag
    18121 ttatcccaaa tgatggagaa gttatagagg gcatagcttc tgtggatgag tcagcaataa
    18181 ctggagaatc tgcacctgtc atgagagaat caggtggaga tttcgcatct gttacaggtg
    18241 gtactacagt tgtaagtgac tggttaaaga taagaataac aacaaaacca ggagaatcgt
    18301 tcttagataa aatgatagct cttgtagaag gtgcttcaag acaaaaaacg ccaaatgaga
    18361 ttgctttaaa tactttgtta gtagggttaa cacttatatt tatggttgtt ttaatatctc
    18421 tttatccaat ggcaaactat gcaggtgtaa agattccaat ttctactatg atagctcttt
    18481 tggtatgttt aataccaact actataggtg gattgttgtc agctattggt attgcaggta
    18541 tggatagagt aactagattt aatgtaattg caatgtcagg aaaagcagta gaagcttgtg
    18601 gagacgttga tactatgatt ttggataaga caggtacgat tacttatggg aatagacttg
    18661 catcagaatt tatagcagtt ggaaatgcag attcaaaaga tttaataaat tattctgtta
    18721 tgtgttcatt aaaagatgat acaccagaag gaaaatctat agttgattta ggtatgaaat
    18781 taggttcaac aggtaagaca aaagaggcag aggaagctga gtttgtagaa ttttctgcac
    18841 aaactagaat gagtggtatt aacttagctg atggtagagc tataagaaaa ggtgcttatg
    18901 atagcataat aaaaagagta aaagaatctg gtggtacagt gccagaagat ttagaagaaa
    18961 atgtaaatcg agtagctaaa ctaggaggaa cacctttagt agtgtgtgta gataatgaga
    19021 tatatggaat tatctactta aaagatacag taaaatcagg gcttgtagaa agatttgcta
    19081 gacttcgaga gataggaata aagacaataa tgtgtacagg ggataatccc ttgacagcag
    19141 ctacaattgc aagagaagct ggagtagatg gatttattgc agaatgtaaa ccagaagata
    19201 aaatagatgc tataaaaaaa gaacaattag agggtaaaat agttgcaatg acaggtgatg
    19261 gaacaaatga tgctcctgca cttgctcagg cagatgtagg tcttgcaatg aatagtggta
    19321 caacatctgc aaaagaagca gcaaatatgg tggatttaga ttcagaccca actaagatat
    19381 tggaagttgt tgaaatagga aaacagcttt taataactcg tggggctttg acaacattta
    19441 gtatagcaaa tgatgttgca aaatattttg cgattatacc ggctatattt actcttgcaa
    19501 tacctcaaat ggagatatta aatgtaatga aactttctac atcatttagt gcgatattat
    19561 cagctttaat atttaatgct ataattatcc catgtttaat acctcttgca atgaagggag
    19621 taaaatatag acctatgaaa tcacaagcta tgttaatgag aaatatgctt atatttggtc
    19681 ttggtggagt tatagtacca tttttaggaa taaaattaat tgatttattg attacacctt
    19741 tagtaatgat tcttaattta ggttaataaa tggaggatat aaaaatgaaa acattaaagc
    19801 cagcattact tgtaagtatt gtattgttag ttgtttgtgg acttgtatat ccacttgttt
    19861 tgacaggtgt aagccaagtt gcatttaaag ataaagccaa tggaagtatg atagaagtaa
    19921 atggtgtaaa agtaggttca gaacttattg gtcaaagctt tacagatgcg agattcttta
    19981 agggaagagt atcatcagta aactataata cttatacaaa agaagattta gtaccagata
    20041 aagatggaaa tgcatcttat ggaggtgtat catcagggtc ttttaattat ggagctacaa
    20101 atccagaact tcatgataga gtacaaaaag atatagaaaa gtttttaaag gataatccaa
    20161 ctgtcaaaag agaggatata ccaacagatt tacttacagc atcaggttca ggacttgacc
    20221 ctaatataag tccagcctct gcaaaaattc agataccagc tatagcaaaa gcgtctggaa
    20281 tatcagaaag taaattacaa aaaattgtag atgataatac ttccaaaaaa ctttttggag
    20341 ttttaggtga agatagagtt aatgtactaa aggttaatat agaggtcgca aaaatattag
    20401 gtcttatata gtattttgtg gaattttagt aacaaaatat tactcaagat ttaatttaaa
    20461 ggttaaattt taaagagtga aaatcaataa tgattttcac ttttttattt gaatattact
    20521 aatatttgga ataaagtgat tgtaagtata aaatccaaaa aaaggttgca ttatcaaaat
    20581 gataatacta taatatattt atcgttatca atttgataat agatttggac atataggagg
    20641 aaatagtatg ttatataata acgcattaga gttgatagga aacacacctg tagtaaggct
    20701 gaataattta ggctgtacta atgggtgttc taacatatat gtaaaattag aaaaagtaaa
    20761 tccagcagga agtataaaag atagagctgt ttatgaaatg ttagagggct tagagcaaag
    20821 aggagaatta aaaaagggag atgtactagt tgaagcaact agtggaaata ctggtattgc
    20881 attatctatg ataggaaaat taaaaggata tgaagtagta atcgtaatgc ccgaaacaat
    20941 gagcgttgag agaagagatt taatgaaggc ttatggagca aatttggtgc ttacagatgg
    21001 taaagctgga atggcaggtt ccatagaaaa agcaaatgaa cttttaaaag aaaatccaaa
    21061 ctataaaagc ttaaaacaat ttgaaaataa agataatcca aatgcacatt ataaaacaac
    21121 agcagttgaa attatgaatg atgtcaaaga tttagatata ttcgtatgtg gagttggaac
    21181 aggtggtact ttaataggaa ctgcaagata tttaaaagaa caaaatccaa acataagagt
    21241 aattgcactt gaaccaaaaa aatctccagc tatatcagaa aacaagactg gacctcataa
    21301 aatacaagga ataggtgcta actttgttcc tgaaaattat gattctagtg tagttgatga
    21361 agtaatatta gtagatgatg aagatgcatt tgatacagtt aaattacttg cagcaaaaga
    21421 gggaatttta gtagggatat cttctggagc aaatatattt ggtgctatag agatggcaaa
    21481 aaagtatcca gataaaaaga tagttacagt agctccagat ggagttgata agtatatgtc
    21541 tatgggaata ttttaataat tttatttgag ggagtgaata ctttgtttaa gaaaataaat
    21601 aaagatattg aatatataat gaagaatgat ccagcagcta ggtctaagat agaagttttt
    21661 ttactttatc catcagttca tgcaatgata atgcatagga tggctcatgc tctttataag
    21721 aagaagaagc tttttacagc aagattaata tcccagatat ccagatttat gactggcata
    21781 gaaattcatc ctggtgctaa aatgggggaa ggtatattga tagaccatgg aatgggtgtt
    21841 gtaattggag agacagctga agtaggtaat agggtgacaa tatatcaagg agctacttta
    21901 ggagctacag gaaaagatac tggaaagaga catccaactg ttggtgatga tgtattaatt
    21961 ggtgctggaa caaaaatact aggaccttta aatataggtt caaattcaaa aattggtgca
    22021 aattctgttg tcgttaagga tgtacctaat ggtgcaacag ttgttggtat accagcaaaa
    22081 atagtaaaaa taagaaacct agaacctgta aaaaagaata aaaaagaagt gtcctatgag
    22141 tatgatgaat tagataatgt atattatata taggatttat ataactataa aataaacttt
    22201 ataaaaatta ttttaaaagg tatttttttc aaatttgagg acataataaa aaagaactga
    22261 atgttacagt tttggttcat caatctttga taaacaagat tttaaatttg tagcgtctag
    22321 gaggatttta ttatgaaagc aaatgttaat caagatactt gtataggttg tggattatgt
    22381 ccatcaatat gtccagaagt atttgatatg aaggatgatg ggaaatcaca tgttatagtt
    22441 gatgaagtac caagtgatgc tgaagaatca gcagcagaag ctagagaaag ctgtccagtt
    22501 gatgcaatag atgtgcatta ataaaataaa tgcctataaa aattgaattt tcaattttta
    22561 taggcatttg tcttttattc agttagtatt tcaacccctg tttctgtaac taaaaccata
    22621 cattcccatt gagcagataa tttaccatct tttgtataaa tagtccaatt attttcttca
    22681 tcaacaaaaa tttcgtgagt tccttcattt accataggtt ctattgtgaa tatcatccca
    22741 ggaaccaata gcatctcagt ccctttttta gatacataac ttacaaaagg gtcttcatga
    22801 aagcttaatc caactccatg accacctatt tctctaacaa cagagtatcc atttttcttt
    22861 gcataagagt tgatagcatc acctatatct cctaaaaatc cccatggttt ggcaacttca
    22921 agaccttttt ggacacattc ctttgtaact tgaactaatt tttttgcatt ttcattaaca
    22981 ttacctatca taaacattct tgaagcatct gaatagtatc cattaaaaat agtagaaaca
    23041 tctacattta taatatctcc atcttttaag ataatgtcct tgcttggaat accatgacaa
    23101 acttcattat ttactgatgt acatacactt ttaggaaatc cattatagtc tagtggagca
    23161 gggatagctc cattttgtag tgtaaagtca tgaaccaact tattcatatc ttctgtagac
    23221 attccaattt cgattttatc tgaaatataa tccaaaattt ttgtgtttat tacagcactt
    23281 ttttttattt cctcaatttg actttcattt tttataatat catgagttgg tacaatatga
    23341 cctaacttag caaatgaatc tattttatta tccatatcca tatgacattt tttatatttt
    23401 ttagtacttc cacaccaaca gctatcattt ctatttaact tcataacttt tcatccttct
    23461 tttattattt atttaaatta tgggttacat aaactatttt aaaacataac cattataaat
    23521 attataacat aaatactaag aaatccttta tagatagtga tttattcaaa gtataaatta
    23581 ttatatggtt attgttgatg aaaagaggat agttaaatgt attattggat attagcgcac
    23641 ttaatagttt agttcaagtt tttcaagaaa atcatatgtt agaggacaat tttcctttaa
    23701 agtatttttt gaatcttcat caagataata atgcacaaat gattctgcaa aaaattcttc
    23761 tataaacttg cttatatatg catgtgcaac ttgtttaggg tcataaagct tatatctttc
    23821 ctgagcaaat acttccataa attcttcaga atttgatttt ttatttaaaa catttttatc
    23881 tatagcatgt gcagtttcat gtaattctaa attttttgat gaatgtacat ttgcatagct
    23941 agggttacta taacctattt tagctactat agaattattt ctatatatac caggtacatc
    24001 tttccaagtc ttgcctgatc ttacccaaca tggaggtaat tgatatttta aatcactaaa
    24061 ttctggttca tcagttagat ttgaatttat taattttatt tttatatcct tttctttaag
    24121 tgtctgtaaa tatttaatat ttatttttgc aagtctgctt ttcatatttt caacttcatc
    24181 tttatcataa ttcaagtcat caggaagtat aagcatttgt tcaattatat ctatgctact
    24241 gtcttttata tcttcagatt cttcatcaaa tttatattga agagtattat tttcatagat
    24301 agttatattt gtattatttt ccatataata agagtgaata tcctctaagt ctaaaatatt
    24361 catttgttgt aaaatatgtt ttgtttcgcc actccatact actaaagaaa gtgaattaaa
    24421 attaattagt ggattttcct ttgcttttat atcattgctt atatctacat caaatacata
    24481 ttcaattgag tcttctatgt taagactatc tgtattcttt ttaatatata aatatgcttg
    24541 ttttaagtct atataaatct catctaaaga ttcatcataa aattctgata aattattagt
    24601 aaagagttct tcacttaaaa gttcattttt atcagttata ctttcaatta aaatattaat
    24661 ttcttttatc atatcgtaaa tacttatttt tctatttgct ttttcactag atacattgca
    24721 tggcgcaaga gcaatgcaat aaaaatcttt ttctttttta taaaaggctt taagtgattt
    24781 tgataaaagt gtaaagtcta cacctatctg taagttgacc ttaagtgagt cattttgtaa
    24841 taattcagta ttattttttt tagctatatt taaattgttt tccataatca ttgccccctt
    24901 tttattaggg tatcataatt tttttatatg tgtcaatttg tttttttgaa aattataatt
    24961 tttttgaaaa ttttattttt tataatttgt gaatttttta ttatagaaat aaatatgtta
    25021 ttattatgtc aaaatttagc attagatt
//
LOCUS       DAMJVX010000040        24317 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMJVX010000040 DAMJVX010000000
VERSION     DAMJVX010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24317
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.042"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            109..300
                     /locus_tag="PC407_001519"
     CDS             109..300
                     /locus_tag="PC407_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDF2496971.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            455..1864
                     /locus_tag="PC407_001520"
     CDS             455..1864
                     /locus_tag="PC407_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2496972.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIRIFKDKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKICGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            1995..2684
                     /locus_tag="PC407_001521"
     CDS             1995..2684
                     /locus_tag="PC407_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDF2496973.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2687..3622
                     /locus_tag="PC407_001522"
     CDS             2687..3622
                     /locus_tag="PC407_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDF2496974.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3619..4512
                     /locus_tag="PC407_001523"
     CDS             3619..4512
                     /locus_tag="PC407_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDF2496975.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4590..5336
                     /locus_tag="PC407_001524"
     CDS             4590..5336
                     /locus_tag="PC407_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDF2496976.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRASSRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5337..6170
                     /gene="rsmI"
                     /locus_tag="PC407_001525"
     CDS             5337..6170
                     /gene="rsmI"
                     /locus_tag="PC407_001525"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDF2496977.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6193..6897
                     /locus_tag="PC407_001526"
     CDS             6193..6897
                     /locus_tag="PC407_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDF2496978.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7085..7513)
                     /locus_tag="PC407_001527"
     CDS             complement(7085..7513)
                     /locus_tag="PC407_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF2496979.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7550..8980)
                     /locus_tag="PC407_001528"
     CDS             complement(7550..8980)
                     /locus_tag="PC407_001528"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDF2496980.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9112..9696
                     /locus_tag="PC407_001529"
     CDS             9112..9696
                     /locus_tag="PC407_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HDF2496981.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9712..10233
                     /locus_tag="PC407_001530"
     CDS             9712..10233
                     /locus_tag="PC407_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HDF2496982.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10299..10545
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10629..12566
                     /gene="metG"
                     /locus_tag="PC407_001531"
     CDS             10629..12566
                     /gene="metG"
                     /locus_tag="PC407_001531"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDF2496983.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12596..13366
                     /locus_tag="PC407_001532"
     CDS             12596..13366
                     /locus_tag="PC407_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDF2496984.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGICYEEVCRVTKENAKKFFNIK"
     gene            13703..14134
                     /gene="phnG"
                     /locus_tag="PC407_001533"
     CDS             13703..14134
                     /gene="phnG"
                     /locus_tag="PC407_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDF2496985.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14149..14724
                     /gene="phnH"
                     /locus_tag="PC407_001534"
     CDS             14149..14724
                     /gene="phnH"
                     /locus_tag="PC407_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDF2496986.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTVSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSTINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14727..15812
                     /locus_tag="PC407_001535"
     CDS             14727..15812
                     /locus_tag="PC407_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HDF2496987.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRDFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15832..16701
                     /locus_tag="PC407_001536"
     CDS             15832..16701
                     /locus_tag="PC407_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDF2496988.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16694..17560
                     /locus_tag="PC407_001537"
     CDS             16694..17560
                     /locus_tag="PC407_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2496989.1"
                     /translation="MSKINVSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17601..18314
                     /locus_tag="PC407_001538"
     CDS             17601..18314
                     /locus_tag="PC407_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2496990.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMAKGTISESGVA"
     gene            18315..19484
                     /locus_tag="PC407_001539"
     CDS             18315..19484
                     /locus_tag="PC407_001539"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDF2496991.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDRIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVLNTIVNGKTVYKREV"
     gene            19488..20324
                     /locus_tag="PC407_001540"
     CDS             19488..20324
                     /locus_tag="PC407_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2496992.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20325..21320
                     /locus_tag="PC407_001541"
     CDS             20325..21320
                     /locus_tag="PC407_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2496993.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLDVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21338..23026
                     /locus_tag="PC407_001542"
     CDS             21338..23026
                     /locus_tag="PC407_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2496994.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYIIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23038..24105
                     /locus_tag="PC407_001543"
     CDS             23038..24105
                     /locus_tag="PC407_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2496995.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
BASE COUNT         9705 a         2709 c         4344 g         7559 t
ORIGIN      
        1 ttttttgtca aaaaaaacaa aaaatacgta tttatttttt catttcggga aaactaaaca
       61 caggaattat ggactttata aaaatttaat tcttgaggag tgatataagt gaaaaaagtt
      121 gctttaattt tagctataat aggtgcgctt aactggggtg caattggtat tctaggtaca
      181 gacttaatag gtagtatctt tggtggaaca tatgaaatgg taagtagaat aatatacttc
      241 attgtaggtt tagctggatt atacttaata ccaagtttaa tgtctgatga tagagaataa
      301 tttatgcttt tatgttaaat acctcattct aaatgtttag aatgaggtat ttaattttat
      361 aaaaatatta atttgtttct tttttaatgg taaaatagat aagaattgaa gttttaaaaa
      421 gaagtattat gtaaaaaagg agtactatta atatatgttt attattaatg aattaaataa
      481 gattgtaaat gaagaagtaa tatcttttca tatgcctggt cataaaaagg gacaggttta
      541 taaaaagtta ggatataaag atatactaaa aaatttgtat aaaatggata caactgagat
      601 attaggaaca gataatttac actttccgga aggaattata aaaaattcac aaaaaaaagc
      661 atcaagagta tttaaaagtg attatacttg ctatttagta aatggtagta gctgtggaat
      721 acaatctgca ataatgtcag tatgtagtcc taaaagtaaa ataataacaa atagagactg
      781 tcatcagtct gttataaatg gatgtatttt aggagatata gatatagagt atgtgccttg
      841 tgagataagt gaagagacaa atgtcttaaa agggatatct ataagtaatg tgattgatat
      901 aatagataaa aacttggatg caaaagcagt actgcttact tatcctacat attatggaat
      961 gacatatgat ttagaataca tatgtaatta tgcacatagc aaaaagatgg tagtgatagt
     1021 agatgaagca catggtgcgc atttagggct tagtgagagg cttcctaaaa cagctcttga
     1081 gcaaggtgca gatatagttg tacagagtac acataaaact cttccagcat ttacacaatc
     1141 gtcaatgata catatacagg gagaccgagt tgatatagat aaaatattga acatgttaag
     1201 aattacagaa tcttcaagcc cttcatattt attattatct tctctggaat tagcagtaga
     1261 tatatatgaa aataaaggaa aagaattaat ggaagagctt ttaaataata ttagaatttt
     1321 caaagataaa gttgattcaa atattaatat atatgacaca aatgataaaa caaaaatatt
     1381 tatatcatct aaaaacatgg gtttaacagg ttatgaacta gaaaatattt tgagaaacaa
     1441 atataacata caagtggagt tatctaatta ttatggagtt cttttaatat gtacaatagg
     1501 aaatactgct gaggatttct ctagtttaga aacagcatta aacaatgttg taaaggaaga
     1561 ctttaaattt gaaaagttag aaaataaaaa ataccctgtt gttattccaa agaaaatatt
     1621 aacacctaga gaagcatttt ataaaactaa aaaaagtgtt aaaatatatg acagcatagg
     1681 taaaatttgt ggtgaatgta tagttccata cccacctgga ataagtgtaa tatctcctgg
     1741 tgaagtaata agtaaagaaa taatagacta tctaatgttt tgtcattcta aaggcatgat
     1801 tgtgagtgga ttgaaagatg tagatttaaa ttttatacaa gtaatagatt tagaagacat
     1861 ttaaaaatta tgatataaaa gtagatagaa gtagatagtt taaaattgtg aagagaaata
     1921 aatagactgc ttatatagag ctatctaata aaagtactaa aaaagtaaaa agtgtttgta
     1981 tggaggatat gaaaatgaaa ggtaagttaa taattataga gagtggttca gatgcaagtg
     2041 gaaaggctac tcagacagaa atattatatg aaagattaaa aaaagaaggc tatatgatta
     2101 aaaaggtaga atatccaaat tatgaatctc catcatctgc cttagtgaaa atgtacctaa
     2161 aaggagactt tggtaaaaat gcatcagatg tagacccata tatagcatca acttttttta
     2221 cagctgatag gtatgcatca tttaaaaccg attgggagga gttttacaat gaaggtggaa
     2281 taattatcgc agatagatat acaacctcta atatggtaca tcaggcctct aaaatggata
     2341 tttgtgatag agaagagtat ttaaattggt tagctgatta tgaatttaat ttatttaaga
     2401 taccacaacc tgattgtgtt atatttttag atgtgcctat tgaatttagt aaaaagctta
     2461 tggagaatag aaaaaataaa attacagggg aagaaaaaaa ggatattcat gaaagtgaca
     2521 tagagtattt agagaaatct tacaacaatg ctttatatat agcagataaa tatgattgga
     2581 aaaaaataaa ttgtgtttta gataacaaat taagaagtat tgaaagtata cataatgaaa
     2641 tttataaaat agttcttgat tcaataaaac ctatggagag ataaatatgt attttgaaaa
     2701 tattataggg caagattttg caaagaaata tttaacaaat tctataaaga aaaataaact
     2761 taataatgcg tatatgtttg aaggaatgga tggaatagga aaaaagaaat ttgcagatga
     2821 attatcaaaa ctattacttg attatgaaaa tctcgaaaat agtccagact atgtactcat
     2881 aaaaccagat ggaaatagta taaaaatagc tcaaatcaga aatttacaat cagatatagt
     2941 aataagacct cataaagact ataaaatata tataatcaat aatgctgaaa aaatgactgt
     3001 ggaagctcaa aatgcacttt taaagacgct agaggaacct ccaaactatg ctataataat
     3061 cttagtcaca aacaacaagg aatctttatt agaaactata aaatctagat gtgatataat
     3121 aaagttctca cctataccaa ttgaagactt aaaacgttat ttgataaata caggtataga
     3181 agaagaaaga gctcaattat tggctatttt ttcgagagga agtattgaga atgctctaaa
     3241 tctatctcag tcttctgagt tttcaatgat gagagaagat atacaacagt atattcagat
     3301 aatgttagat aaaaacatag tggagatttt aaatatacca aacaatatgg aaaaatatag
     3361 aggtaagatt atagctttat tagatatgat gataaactat tttcgagata tcatcctatt
     3421 aaaagaaaat gtaaacaaga acatgttaat aaatgtagat aagctagttt tcattcaaaa
     3481 tatgagtgga aaaattagtt attctcaatt gtccaagatt attgatataa tagaagatgc
     3541 aaagagtaaa ataaaaagca attgcaattt caatataagc atacaagtta tgtctttaaa
     3601 tatatacgag gtgattaaat gataaagata gtaggtataa ggtttaaaag tgctgggaaa
     3661 atatattatt ttgaccctgt tgattttaat atagagcaag atatggatgt agttgtagaa
     3721 actgcaagag gcttggagta tgggaaagta gtcgttggtc caaaagatat ggatgaaagc
     3781 gaattagttt ctcctttgaa gccaattata agaatagcta cagaagaaga taaaaaagta
     3841 tatctagaaa ataaggaaaa ggcaaaagaa acttttgagt tgtgccaaca aaaaataaaa
     3901 gaacatgagt taacaatgtt tttaatagat tgtgagtata cgtttgatag gaataagttg
     3961 attttttact ttacagcaga aggaagaata gattttagag aattggtaaa agacttagct
     4021 gctatattta aaactagaat agaacttaga caaataggtg tcagagatga agctaaatct
     4081 ataggaggtc tagggccttg tggtagaaaa ctttgttgtt catcttggtt aggtgatttt
     4141 caaccagttt caataaaaat ggcaaaagac caaagtctat ctctaaatcc aacaaaaata
     4201 tctggaatat gtgggagatt attctgttgc ttaaaatatg agcatgatgt atatagtgaa
     4261 gcaatagaaa aaatgcctgt tgtaggtgca ttggtgcaat cagatgatgg aaaaggaaaa
     4321 gttgtagaag tgaatccttt attagaacaa attaaagtgg aatttcaaga caagacagta
     4381 aagatataca caagagaaga tataaaaata cttagagagc ctaaaaaatg tgatggctgt
     4441 ggtggtaaat gtaaggataa tgatggatta gatgaagcca cactaagaga gctaaagaaa
     4501 ttagaagatt aaatttaagg tagggctttt cctacctttt ttaaagcaat taatgcttaa
     4561 agttattaat atttgaggag aatttatata tgcaattaaa agattcagaa agaatagatg
     4621 atttacaatt aaaaggcctt aaaattatac aagatactaa tggtttttgt tttggtatag
     4681 atgcagtttt attagccaat tttactaaaa taaaaaaaga tgccaaagta gtagatttgg
     4741 gtactggaac tggaattata cctattttga tagctggaaa aagtgaagct aaaaaaatta
     4801 ttggcgtgga aatacaagaa gatgtatatg aaatggcaac tcgttctata aaattaaatg
     4861 gattagaaga aagagttgag attataaatg aagatataaa atacatagac aaagtcttag
     4921 acataaatgg ttaccatgta gtcacatcaa atcctccata tatgcatatt gatggaataa
     4981 aaaatccaaa tgataaaaaa gcaatatcaa gacatgaagt aaggtgcaat ttggaagatg
     5041 taattagagc ttcatccaga ttggttatgc ctagaggaaa attcttcatg atacatagac
     5101 caacaagatt ggttgatata ataactttag ggagaaaata taaattagaa ccaaaggtta
     5161 ttcaatttgt gcatccaaga cctcaaaaag caccgaactt agttttggtt caatttgtaa
     5221 aagatggaag accagagctt aaaatattgg acccattgta tgtttatgga gaagatggga
     5281 attatacaaa agaattaaaa gcaatatata ataataaaga tataggagag ttgtaaatga
     5341 gtgggaaatt gtacatatgt ccaacaccaa taggaaacct agaagatata acatatagaa
     5401 ctttaagaac attaaaagaa gtagacttga ttgcagcaga agatacaaga catagtataa
     5461 aacttttaaa tcattttgaa atctcaaaac cactaactag ttatcatgaa cataataagg
     5521 attcaaaggg agattatttg ataaataaac taattgatgg tgaaaatata gctctgataa
     5581 gtgatgcagg tatgcctggt atatctgacc caggagaaga gataataaaa caagctatac
     5641 aaaataacat agagatagaa gtattgccag gtgcaacagc ctttgtaacg gctttagttg
     5701 gctctggtat ggacacacat agatttgttt ttgagggctt tttagataga gataaaaaag
     5761 ttaggaaagc acaattggaa gaattaaagg aagaaagtag aactataatt ttctatgaat
     5821 caccacatag attgaaggat acactaaaag atatgcttaa aattcttggt aataggaata
     5881 tttctataaa tagagaactt acaaaaaaat atcaagaaat aataagagaa aatatagaaa
     5941 ctaccataaa aatatttgag gaaaaagaag taagaggaga gtttgtatta atagtagagg
     6001 gttttcatgg tgagaagtca aagaaagaaa attatgatgg gcttacagat agggaatatg
     6061 ttttaaaact aattgaaagt ggtatgaaca aaaaagatgc aataaaaata gtttgtaaag
     6121 atagaaagtt aaagaaagat gtggtttata aacaagtttt agatttataa aacctgaatc
     6181 tgaggtgatt ttatggaaat agggaaaatt ataatagaga gtttaaagag ggaagataca
     6241 cataaaatat ttatagaatt agataataag catgaattag aaaaaataat tcctaaaata
     6301 agctgtatga agtctgtagg tgaatgtaaa tatcatgtag taaattgttt tgaacattct
     6361 ataaatgcat taaaagaatt agaaatagta ttaaatgata aggatttttt tccaatacat
     6421 ctaagaaaac atgtgtcaaa ctatttaaac acatatattg aagaaggaat aaataagtta
     6481 catgtattaa agttgggaac atttttacat gatataggta agcctgatag tatgacctta
     6541 gatgaaaccg gaagagttca tttcacaaat catgaaaaaa taggtgctca aataattgac
     6601 aatatgggaa taaagttaga gttatcaaca gagacatata gacttataag taaatatgta
     6661 agatatcata tgatactttt gtctctatat aagaaaaatg atttgagtag gaaagaatta
     6721 attaatgtat ttaacttagt agatgaggac actataggtg taataatgct tgggtatgca
     6781 gatatagtgt ctacagtaaa attattgggc aagagtggag aagtcagtgt gttaaagaca
     6841 tatatggaat atattttaac aaattacttg tataagagta attatacaca tgtataaatt
     6901 tagttaatat aattgagttt tagatgatta attgaagttt gaaatatacg gatttcaaaa
     6961 acataaaaga agttgtcttt gttgtaaaat tatgaaagac aacttttttt atatcaaaaa
     7021 ttgaaatttt attaaggata cttaatggtc tattaattaa taaaccatta gtatttaatt
     7081 tatattacaa tattgatttt gcttgtattg gcgttgatag tataacagag gtcttagtag
     7141 aaccaatttt ctttatagta tcaatcacat tttctagctc gtacatatct tttacaatta
     7201 cttttaaaag taagcaatca tcacctgtta tatggtgaca ttctacaatc ctagggtcct
     7261 ttgcagctga ctcaataaat tctgtatatc cattgcttgg aagagaaata tgaataaatg
     7321 cctttataac tctgcctaat gaatctgggt tgacaatagc tttatatcct tctataacac
     7381 cagactcttc taatcttttg actctttctg aaactgcagg agaagttaaa ccaactattt
     7441 ttcctaagtc cttcatagaa attctcccat catcctgtaa aatttctatg attctgtaat
     7501 ctgtaacatc cataaataaa cagtccccct taaataaaaa taattaatac tataaaactt
     7561 ttttagaatc tatcatttgt gtttttaaat accaaagatt tctagataaa tcaaccttat
     7621 aaatgtgtgg ttttgaaagt cttttgtatt gttcccagat agtacttaat tcatattgta
     7681 catgattttt tatttcaaga acagcttttt tattatattt gcatctgcct tttatataca
     7741 atggtttaag aagctcttta actttgtaat ttgtaaatac ctttgttttc catgtataag
     7801 taggatggaa gatttctaaa ggtttagact catctataac ttcatcatgt aacataatca
     7861 aatctgcctc tgccttattg tcttcgttat atattcttat aactttcttg taaccaggat
     7921 tatttatttt ttctggattc tcagaaatct ttatcttagg ttcaagaact cctttttcac
     7981 aagatgcagc taatttataa actcctccaa gagaaggtga atcgaaagat gtaatgagct
     8041 ttgtacctac tccccatgaa tttatagtag ctccttctgc tttaagtgat gttatggtat
     8101 attcgtctag gtcattagat gcagtgatgc tgatatctga aaagccagcg ttatctaatt
     8161 gttttttagc ttctttagat aaataagcta aatctccaga gtcaagtctt atacccatag
     8221 gtttgtatcc tttttcagaa atatttttga atacttttat tgcattagga actccactgt
     8281 ttaatacatc ataagtatct acaagtagta ggcatttatc tggataaaca tcagcatatg
     8341 cttggaatgc ttcaagttca ttatcaaatt tttgtaccca actgtgagct tgtgtaccaa
     8401 ttataggtat atcaaacatt ttccctgcta gtacgtttgc agttccagca caaccaccaa
     8461 ctacagcagc cctagctcca tatatccctg catctggacc ttgtgcacga cgtaatccaa
     8521 attcaaatac agggtctcct tgagctgcaa agcaaactct agaggcttta gtagcaataa
     8581 gagactgaaa attaactatt gttaacaatg ctgtttcaat aagttgagct tgatataaag
     8641 gtgccttaac agtaattaat ggctcattag ggaacatgat agttccttcc tcaacagcat
     8701 atatatctcc tgtgaattta aattctttta aaaattttaa aaacttgtca gaaaataaat
     8761 ttaagttttt taagtactct aaatcttcat ctgaaaaatg aagattatct atgtattcaa
     8821 caacctgatc aataccacaa actatagtat accctccatc acaagcgttt tttctaaaaa
     8881 acatatcaaa tacaacaatg tcttcatgaa tattcttttc aaaatagcca tttaacatag
     8941 taagctgata taaatcagta agaagtgtta gatttctcat tatttctctc cttaattatt
     9001 attaaatatt aaaaattgta aaaactccca cacttatact aaaacataat atagtacaac
     9061 tttcaagtat aaagtattac ctaactatca taaaattatt taaagaggag gatgattata
     9121 ataggaaaaa tatggtttta tatggtatcg ataggaataa taggaagctt gatttctaaa
     9181 aatatgggtg aattaaataa agttatatta agtgaaacat caaaaggtat agagtttgca
     9241 ataagtctag ctggtatcat ggcactctgg atggggataa tgaatatcgc aaaagaatca
     9301 ggtttaattg aaaagatagg tcaaaaacta aatcctataa tgagaagatt atttccttct
     9361 attccaaagg gtcataaagc aatgtcttat atagttatga atattgcttt aaatatgttg
     9421 ggtgctggaa atggtgcaac agcatttggt ttaaaagcaa tgagtgagct tcaaactttg
     9481 aataacaaga aagatacagc cactaatgat atgattatgt ttatggtaat caatatatca
     9541 tctatacaaa taattccatt tactataatc aaattaagga tggatatggg ggctcaaaat
     9601 ccaagtgaaa taatttttac gactttattt gcaaccataa tatctacagc agtagcaata
     9661 ataacatgta aattttttca aagaagatat agataaaggg gatgtagata catggagata
     9721 ttttcaaact tacttatacc aataataatt ctgtacatag tagtttatgg taaatataaa
     9781 aaaatagatg tgtatgatag ttttgtaaaa ggagcaattg atggattaaa agcagcatgg
     9841 gatatcctac catatataat aggtatattt ttggcaatag gaatattcaa aactggtaaa
     9901 ggtcttgata tgttagaatg gctatttaca ccaatagcaa atatgatgag tataccaaaa
     9961 gaattaatag gtctaataag tgtaaaacct ttatctggaa gtggtgcttt agggatgtac
    10021 agtgaacttg caaatagagt tggaatagga agtttagtag aaaaaatggg tgctacgatt
    10081 gtaggttctt ctgagactat tttttacaca atggcaatat actatggaag tctgaagata
    10141 aaaaatacta gacatacatt atcatgtgct atgattagcc atgttgcagg tgtcatagca
    10201 gctgttttta tttgctatgt aatatttgta taatttgttg acaatatggg aaaaatttta
    10261 taatatagtg attatataga aacaaatagt aatacaaatt acaagctatg aaaagaagag
    10321 taaataaagg ttttttctat cagagagcta ggttaggtga aagctagtgt caaaagcttt
    10381 attgaagatg gtcttggagc tttttatctg agcaagtgtg ctagggtaaa acggttggac
    10441 acgttatagt ctactaaggt atctataaaa tgtttctaca tttcatgtga aacataacta
    10501 ttatagatga attagggtgg taccgtgaat aatctcgccc ctatgttaac tcataggagg
    10561 cgatttttta ttatttaaat gaaagaatca tatgattacg aacgataaaa aattaggagg
    10621 taaataacat gagcaaaccg agtttttatg taacaacacc aatatactat ccaagtggag
    10681 gattacacat aggacatact tattcaacag tagctgcaga tacaatagct agattcaagc
    10741 gtttctgtgg atatgatgta aagttcttaa caggaacaga tgagcatggt gaaaaaattc
    10801 aaaagaaagc catagaacaa ggaatgtcag agatagagta tcttgatgga atgataaaag
    10861 atataaaggc cttatggaat actatggata tatcttatga tgattttata agaactacag
    10921 aaaaaagaca tacagacata atacaaaaga tatttactaa actatatgag caaggtgata
    10981 tatataaagg tgaatatgaa ggtagatact gtactccttg tgagagtttc tggacagaat
    11041 ctcaattact agaaggaaat aaatgccctg actgtggaag agaaacatat ttagtaaaag
    11101 aagaatctta tttctttaga ttatctaaat atgaagacag attaaaagag ttatttaaag
    11161 atgatagttt ctgttttcct gctgctagaa aaaatgaaat ggtggcaaac tttttagaca
    11221 aaggattaga agatttaagc gtaactagaa cgacttttga ctggggtata aaagttcctt
    11281 ttgatgaaaa acatgtaatt tatgtgtggg tagatgcttt atgtaactat ataacagcat
    11341 taggttatat gactgataat gatgaagaat ttaaaaaata ttggcctgcc aatgttcaaa
    11401 tagtaggtaa agaaatagtg agattccata caataatatg gccagcgctt ttaatggctt
    11461 taggtctaga agttccaaaa caagtatttg gacatggatg gatactattt gctgatgata
    11521 aaatgagtaa atcaaaaggt aatgtggttt atccagaacc tataatagaa agatatggaa
    11581 tagacacact taagtacttc ttattaagag agtttgcatt tgggcaagat ggaagttata
    11641 ctcatagaaa ttttgtaacg agaataaatt atgatttagc aaatgattta ggaaacttaa
    11701 taagtagaac tgtagctatg gttgaaaaat acaataatgg tataatacca acagcaaaag
    11761 tttcaactga ttttgatgct gacttaaaag aacaagctgt ttcaactaga gaaaactttg
    11821 aagctgaaat ggataaaatg caattccatg aagcattaga gagtgtatgg aaattagtta
    11881 gaagaactaa caaatatata gatgaaacta tgccttgggc actagctaaa gatgaaacga
    11941 agaaagatga attagacaca gttttatata atttatgtga atctataaga ataatcgcaa
    12001 cactaataaa tcctataatg aatgagactg ctaataaaat atatgaacat ataggaataa
    12061 aaggtcaaga tgatataact acatgggaaa gtacaaaaac atttggtctt attggagaaa
    12121 atgtaaaagt atttaaaggt gaacctttat tcccaagatt agatgtagaa aaagagatag
    12181 aagaattaac taaaatgttc tctgggaaac ctccagtaga agaaaaacct ttagagcata
    12241 aagaagaaat tactattgat gatttagaca aaatagagct tagagtaggg aaaataataa
    12301 gctgtgaaaa acatccaaaa gcaaataaat tattagtatc acaagttaag attggaccag
    12361 aaacaagaca aatagtatca ggaatagctg aatactataa gccagaggat ttagtaggaa
    12421 aagaggttac agtggtatgt aacttaaaac cagttaaatt aagaggtgta gaatctcaag
    12481 gtatgatatt agcagcaggt gatgatggag acccatatgt tttaccattt acacaagggg
    12541 ctaaagatgg atgcgaggtt cgttagtagg taattaaata gtaaggagat taaagatgtt
    12601 atttgactca catgcacatt taaatgatga aagttttgat gaagatagag atgaacttat
    12661 tggttcatta aaagataaag gtgtagattt ggttgtaaat ccaggagcag atatagagac
    12721 gtcgataaca gcaattgaac ttgcgaaaaa atacgacttt atatattcag cagttggggt
    12781 acatccacat gatgtatcaa aactagatga tacagcaata gaaactctaa gaaaacttgc
    12841 aactgagaat gaaaaagttg tagctattgg agagattgga ttagattatt attatgatta
    12901 ttctccaaga gaagaacaaa aagagtggtt taagaagcaa attgaattag ctaatgagct
    12961 aaaacttcca ataataatac atgatagaga tgcacatgga gatacttttg agataataaa
    13021 gaatactaaa aatcctgaaa taggatgtgt acttcattgt tatagtggaa atgtagagtt
    13081 agctagagaa tatgttaaga tgggttgtta tatatcaatt ccaggcacgg ttacttttaa
    13141 gaataataaa aaaactagag aagtagtaag agaaatacct ctagaaagat tattcataga
    13201 gactgattca ccatatatgt caccagagcc acatagagga aagagaaaca atccttctca
    13261 agtatctttt gttgctgata agatagccca ggaaaaaggg atatgttatg aagaagtatg
    13321 tagagttaca aaagaaaatg caaagaaatt ttttaatata aaatagacaa attaaatctt
    13381 ataatgatat atataataag tcattataag tataggatga gagtgcctca aaatgagttt
    13441 tttagttcat gaggcactct ttttttatga aatcaaatat attttcatca tttcaacaat
    13501 gaaaaagaag tttatcatat tttgagacag tttgttttta tttgtttaat tcatatttta
    13561 aaatatattt tcttaacatt gttaaaaaag aatttacaag atattcatta gaattaactc
    13621 aactctaata ttcacttaac ttaagtggtt tattatatta ttaaagagaa gtaaacgaaa
    13681 ggtagattaa ggaggctaaa ttttgtatac agttgatagt actaagatat tgataaatat
    13741 gaacttagag aaactagaaa agatatactc aatgataaaa gatacatcaa gtatttctat
    13801 aattaaagaa cctaacttgg ctacagtaat ggtaagagct aatgaaagtg taaaaaatac
    13861 aacatttaat ttaggagaga tattagttac tgaatgtagt gtaaaggtag atgaaagctt
    13921 gggttacgga atagtttcag aaaacaataa taagaaagcc atttacttag cagttataga
    13981 tgcagtttta catagcaata acagtaaatt tgatgagtta aagaattaca taaataaaag
    14041 tgtatatgaa gagaacttaa ggtatgaaca agagattatt gatgaattta gcttgattaa
    14101 taaaacgaaa gttcaattca atgctatgga ttaaatagga ggagaatcat ggtaaattat
    14161 acacaagaaa tatatagaaa gatgctagat agtgcttcgt ttcctgggaa attaaataat
    14221 atagataata taaatataga aaataataca aaactattga atggagcaat tggtatagca
    14281 atcactttat tagaccaaga agttaccttc tatatagaaa attatactca aaaagatata
    14341 aaaaatataa gagcgcttac agtgtcaaat caaatagatt atagaaaatc agattatata
    14401 tttttagata ttaattcaga agtggatata ttacagatta aaataggttc ttttgaatat
    14461 cctgatgaaa gtgcaactat agtacatcaa gtaaatgatt tatcaacgta ctctcaaact
    14521 aagtatataa agcttgattt gagtggtcct ggtataaaat ctaaaaatag tatatatata
    14581 aatggaatta gagaagaatt tatagaaaga ttatctacca taaataaaga ttatccaata
    14641 ggaatagatt tgattttagt agataaaaaa ggagaaattg catttatacc aaggtcatca
    14701 aaattaagtt gggaggtatt gtagaaatgg cttatgtagc agttaaaggt ggagaagaag
    14761 ccatattaca ggctaaaaat atacttgagt tttatagggt aaaaggagat agcttgccta
    14821 tagatgtaaa gcagattgag gaacaaatgt cttatgctgt agacaaagta atgtctgaag
    14881 gaagtttata caacaaggaa ctatcagcat tggctataaa acaagctcaa ggtgatatat
    14941 tagaagcctc tttctatgta agagctttaa ggtcaacaat gcctagaatt acatattcag
    15001 aacccataaa tacagaagaa atgagaataa aaagaagaat atcagctact tttaaagata
    15061 taccaggagg tcagtatctt ggggcaacta gagactatga aaatagaata ttaaatgtag
    15121 aattattaga agaagatgaa aaaaagaata gagattttag agatagtttt ataaatgaat
    15181 tagaaacagt agatgaaatc cctgaattta caaaaatatc taatatattt agaaaagaaa
    15241 atttattaca agaagaagtt tgtgaaaaca aattggaaga agttattgat gatattacaa
    15301 gaaatagttt aagatttcca gtttctaggt cagcaaagtt acaggcattg actagaggtg
    15361 aaagtggagt agtgtcaggt tttgcttatg ctattgtaag aggttttggt gatgaacatc
    15421 catatataag tgaacttaga actggtaatg tatttataaa agtaaaacat cccatctatg
    15481 aagatgaaga aataataata ggtgatatgc tggttactga atgtgaaata gtttcaaaag
    15541 catcacaaaa taagactgaa aattctaaaa ctaattcgga agataaaaat aagatgtctt
    15601 taggatatgg tctttgtatg gggaataatg agaataaggc tatatctatg gccatactag
    15661 acaaatcttt ggagccaaat agtgaaaaac tagcacaaga tgaagaattt gtattgcttc
    15721 atatagatgg aatagattca ttaggattta tatctcactt taagcttcct cactatgtag
    15781 attttaaagc ggatgtagaa agaataaaat agtttgtgga ggtaagttta ggtgatatat
    15841 aactatggtt ttttagacga aaacacaaaa aaagaaataa gaagaaagat attaaaagca
    15901 atatcaatac ctggttatca agttcccttt ggttcaagag agttgcctat agctaaggga
    15961 tggggtactg gaggtttaca attaacacta tcattaattg gtaaaaatga tgtagtaaaa
    16021 gtaattgacc aaggaagtga tgattctaca aatgcttgta atataaggag ctttattgca
    16081 agtgtaagta atgtagaaac cacaaaagat acattagaag ctacattgat tcaaacgaga
    16141 catagaatac cagaagaaaa acttaaatct aatcaaatac taatatttca agtaccaata
    16201 ccagaaaccc ttagaatagt agaacctagt gaagtagaaa caagaagaat gcattcagaa
    16261 gaagattata gtaggatgtg ggtatatctt tatgaagata tagttagatt taatgatata
    16321 tctatagcag ttgaatatcc atgtaaagta aatgatagat atttaatgaa tccatcacct
    16381 atacctagat ttgatataaa aaaattaaat atgtcagaca atttattctt gtttggtgca
    16441 ggtagagaaa aaagaattta tgctattcct ccatatacaa aggtagaacc tttagagttt
    16501 gaggattata agtttgaaga ggaaaagttt gaaggaaaat actgtagttt atgtaacagt
    16561 acaaatacat ttttagatga agtatatgac agtgacacta atgaaaagta ctatagttgc
    16621 tcagatacaa gttattgtga aaaagttaga ttaaaaaata acagtataga tgtgacaata
    16681 ggaggtactt ggaatgagta aaataaatgt ctctaaaaga ttagaaaata tagacaagca
    16741 agtattaata ggaagaaata tcaatcttat ttatggagat gggtgtgaaa agtgttttga
    16801 aagcacagga tatgaaagca ataatatatg taagtattgt ggttctatag ttgcatgtaa
    16861 taatgtaaat ttagaacttt atgaaggaga agtccttgga attgtaggtg aatctggttc
    16921 aggaaaaagc actttattaa aaatactgtt tttccaagaa catgcaaata gtggagaagt
    16981 atatatatcc cattacaata aagacacaaa tatactagag ctttcagacc aaaaaaagag
    17041 atacataaag aatcatttta tgggtattgt gtatcaaaat ccccaccttg gtttaaattt
    17101 agactttagt agtggtggta atatagcaga aaaattattg atggcaaatt tatataatgt
    17161 tgaaaaaata agagatagag gcaaagaact tttaataaaa actaatatac ctgtagaaag
    17221 aatagatcat aaaccaaaat actttagtgg gggaatgcaa caacgagttc aaatagcaaa
    17281 agctctttca aataaccctc caatattgct attagatgag gttacaacag gtttagatgt
    17341 ttctgtgcaa gcggaagtat tggatttaat aagagaaatc caaagagaac ttaagatttc
    17401 tatgatagtt gtatcccatg actttgatgt tataaagatg cttgctgata ggacaattgt
    17461 aatgaaaaat ggaagtgtga ttgagtctgg tcttacagac caaataatgg aggacccaca
    17521 acacccatat actcagcaac ttataaattc attgctataa atatcgtttg taaataagca
    17581 aaatgggggt ttttaataaa atgaaagaaa ctatacttga agtaaataat tttagtaaag
    17641 actttgagtt acatgcattg aacaagacta taacggcatg tagtaatata agttttgaag
    17701 tttcaaaagg tgagttttta ggaataatag gtaaatctgg agcaggtaag tctaccatat
    17761 taaaaagtat ctataaaaca tatataccaa caactggaga aataattttt aattcagaaa
    17821 tatatggtaa tgtagacctg gctaaaattg gagaccgaga aatgataaat cttagaaaga
    17881 aagaaatagg atatgtatca cagtttttaa agacacttcc acgaataact gcaattgaac
    17941 tggtagtaca ttctttaatt gagagtgggt ttgaaaaaga ttgttcgtat gatatggcaa
    18001 aagatatact taaccaattt gaaataaagg aaaatttatg ggatgcgtat ccaaataatt
    18061 tttctggtgg agaaaaatta agattaaatt tagctcaagc tatggttaaa aagcctagac
    18121 ttttgttact tgatgaacca actgcatcac ttgataatca atctaaaata tatgtaaaag
    18181 aaaaaatgtt ggaacttaaa agtcaaggaa ctacaatgat aggaattttt catgatatag
    18241 agtttatgga aactgttata gataagacct ttactatggc aaaagggact ataagtgaaa
    18301 gtggggtagc ctagatgata aaactaattc ataatgcaaa tattgtatta gaagatatga
    18361 ttttagaaaa tggtcattta atcatagagg gagatacaat aaaaaaagta tcaaatgata
    18421 ggattgatta cgcatttgac tttaatgaga tagatgaaat aatagatgca aaaaacttat
    18481 atgtaatacc tggtattata gatattcaca gtgatgccat tgaaaaggaa atagagccaa
    18541 gaccaagtac attattacca tttaatatgg cattttatga actagataaa aaactacctg
    18601 caaatggaat aactacagtt tatcattcta tatctttagg ggatggagta ggagttagaa
    18661 gtattgataa ctctttaaag atgattaaaa atattgattc atataaaaat atagatagta
    18721 agagtataaa tcataaagtt catttaagat atgaagtgtt gtattatgaa ggtttagaaa
    18781 aggttttaga actattagat gaaaataaaa tagactactt atcaataatg gaccattctc
    18841 caggacaagg tcagtataca aatccaacat tttataaaga gtatgctact aaagtgtggg
    18901 gagttacaga aaattatgta gatacatggt tagatgattt agtaaatctg catgataatt
    18961 tagattggaa taaaatagct aatattatag gtatagccaa aactaaaaat ataaatgttg
    19021 catcacatga tgatgataca ttagaaaaaa tggatttcat aaaaaatata ggcataaatg
    19081 tatctgaatt tcctataact cttgaagttg caaaacattc aaaaaaattg ggtgttctaa
    19141 cttgtttagg tgcaccgaat attgtaagag gtaaatctca taataacaat ttaaaagcaa
    19201 tggatgctat attggaagat tgttgtgaca ttgtgtgttc ggattatctt ccatcagcta
    19261 tgataaagtc tatgtgtata gttgcagaaa gaataaataa tttaaataaa gcagtcagtt
    19321 tatttacctc aaatcctgct aaagcagttg gtatatatga tgaaagaggt agtataaaag
    19381 aaaataaaaa agcagattta gttttagtag atattgatag tgaatatcca aaggttttaa
    19441 atactattgt aaatggaaaa acagtttata aaagggaggt ataaaaattg ctaacagagt
    19501 tacattgtca tacaagtata tcagataata attttactac tgaagaaata ataaaaaaag
    19561 cagcagaaaa gaatataaat atcttagcaa taacaaatca tgacacatta tctggattgg
    19621 atgaagctct tgtgcttgga gagcattatg gaataacaat aataccaggt atagaaattt
    19681 cagcctatga ttataaaaat agaaaaagag ttcatattct tggatataac atagatttaa
    19741 acagtcaaga aataaaaaat ttgtgcaatc caatggtatg tgacagacat aaagcatctg
    19801 ttaagatggt aaataaaatt atagatttag gatacaaaat atctttggaa gatgtaaaga
    19861 aatattcatc aaagactgga atatttaaac aacatataat gaaagctctt atagataaag
    19921 gatatacaga taatatatat tcaagtctat ataaagaatt gtttcataga ggaaacggca
    19981 aggtatatgt atctttaaaa tatgtggatt acagagatgc aataaaagct ataaaaaatt
    20041 ctggtggaat ttgtgtgtta gctcacccgg gtcaaatgga taattttagc gctattgaag
    20101 agatggtaaa agtaggatta gatggaatag aagtatatca tccatctcac aacaaagaac
    20161 tagaaaaaga gtcgctttct tatgctaaga agtataatct agtggtcaca ggaggctcag
    20221 attaccacgg attttattct caacataatt atgaacttgg aagcaagagc ttaaataaag
    20281 aagatttatt taagtttagg ttagctttag gaggggtatt ataaatgtct tttttacaag
    20341 taaaaaatgt aggtaaaagt tatggacaag ttaaggtgtt aaaagacata tctattgata
    20401 ttgaaaaagg agagtttatt tgtttactag gtccaagtgg ttgtggaaaa agtacactgc
    20461 ttagaataat tgcagggctt gaggataaac atggtggaaa aataataata aatgataagg
    20521 atatgactaa ttcaccacct gaaagtagaa attttggtat agtttttcaa tcatatgcat
    20581 tatttccaaa tatgaatgtt tacaaaaaca tagcctttgg actagaaaat aaaaacataa
    20641 gtaaatctaa tattgataaa aaagttaaag aagttctaga cgttgtagaa ttaagtggat
    20701 atgagaaaaa atatccatca caattatcag gtggacaaca acaaagggtt gcattagctc
    20761 gtgcgattgc tttagagcca gactttttac ttcttgatga acctttatct gcattagatg
    20821 caaaagtaag acttaaactt agggagcaga ttagaagttt acatagaaaa ctaggcatta
    20881 caactataat ggtaacgcat gaccaagaag aagctctttg tttagctgat aagatggttg
    20941 ttatgaatag aggagaaata atacaagttg gaacgccaaa ggaggtatat aaaaatccag
    21001 aaacaccttt tgtagctgat tttataggaa caataaattt tatagatgat ggtattaata
    21061 agatagctat tagaccagaa gatataaaag tagagtctaa tagagattct aaagataagg
    21121 atataaaagt gggagaaatt ttagatatcg aatttagagg atttaactat agaattacag
    21181 ttgagtatcg ttctaagcaa atgaaattag atgtagtttc caaagttgca gaacaaatga
    21241 aactgtgcat aggttctaaa ataaacttta agatacctaa agaaggaatt gtacaatata
    21301 aatcagaggg atgtgcttaa tagctagggg tgaataaatg ataacatcaa aaaataaaag
    21361 aagaataagt gatagcacaa tacaaaaata tttcttgatt ttttcggtaa ttgtactaat
    21421 agtatttata ttatttccat tagtatcatt attaaaaaat gcatttatgg atacaagtgg
    21481 aaattttata ggaatttcaa actttagtaa atatgttgaa aatccaagtc ttatagcatc
    21541 atttaaaaat tcaatttttg tatctacagt ctcatcttta atatcactat gtttagcatt
    21601 tatatattca tacgcaataa caagaacaaa tataaagttt aaagattttt ttaaaactat
    21661 gggaatgttt ccactttttg caccaactat gttatatggt atttcactta tatacctttt
    21721 tggaaataag ggaatcttta caaccatggg tctggaagta ccactatatg gtccattagg
    21781 aataattatt tctcagattc tatttacatt tccacaagct tttttaatat tatctgtagc
    21841 actttcgatg gctgattata gactttatga agcagcagat agcttgggtg ctagtgaatt
    21901 taagaaattt tgcaaaataa caattcctgg tataaagtac tcattactta gttcattctt
    21961 tgtatccttt atacttgcat ttacagattt tggagcagct aaggtagtcg gaggaaacta
    22021 caatgtactg gctactgaca tatataaaca agtagttgga caatttaaca taccaatggg
    22081 agctactgtt tctatggtaa tgttaattcc tgtactaata gcatttacat tagataagat
    22141 ggcaagtaag aaacaaggta tgaccataac tgcaaagtct atgccatata agattaagga
    22201 aaataagttt agagatagag tatttttaat agtatgtagt tttataacat tgctaatttt
    22261 tattttaata ttcgtttcag ttataggttc agttataaag ttatggccat ataatttaag
    22321 ctttacaatg gaacactata catttaaagg tattttggga agtggaataa aaacatactt
    22381 aagcagtttg aaaatatcaa ctttaacagc aatatttgga actatatttg tattttttag
    22441 tgcttatata atagaaaagg tggataagtt tcctaaatta agacaaacag cttacctttt
    22501 atcaatgata ccaatggcct tacctggctt agttttaggt atatcatata taactttttt
    22561 taactcagca agtaatccat taaatttttt atatggaagt acagcaatat tagttatagt
    22621 aaatgtagtt catttttatt cagtttgttt tataacctca aattcatctt taaaaatgtt
    22681 ggataaagag tatgaattag ttgcaaaatc tataaatatt ccattctata aagtattttt
    22741 taatataaca gttccgatgt ctataacatc aatacttgaa atagtagtat attattttgt
    22801 aaattcaatg gttactgttt cagcattaat atttttatat atacctcaaa ctcagactgc
    22861 atcaatatca atactaaagt tagatgagat aggatatata ggaccatcag ctgctatggc
    22921 agtattagtg ctattaacaa atataatagt aagacttttg tacgaatttg taacaaagaa
    22981 attaaaaaat aacacacaaa aatggcagca aaaagatgtt gcatagggga ggaaaaaatg
    23041 aaatttaaaa agctagtttc tttaggagta ttatcaacac ttttattaag tgtaataaca
    23101 ggatgtagta ctgattcatc aggttcagat tcaaagtctg atagtaagac aaaaggggat
    23161 ttaacagttt acacagcaat tgaagaagat agtatagagc catatttagc tacatttaaa
    23221 gagaaatatc cagatattaa attaaatatt gttagggcat caacaggaga tataacagca
    23281 agattattag cagaaaagga taatcctcaa gcagatgttg tatggggagt tgcagccaca
    23341 agcttattgg ttgcagatga ccaggggatg ttagaaccat atgctccaaa aggttctgaa
    23401 gaaatagagt ctgcatttaa agatgataaa gaagttcctt cttgggttgg gatagatgct
    23461 tggatgacag gtataacagt aaacactaaa gaattaagtg ataagaacat cccagttcct
    23521 agttcttacg aagatttaat aaagccagaa tataaaggtc ttatatctat gcctaatcca
    23581 tcttcttctg gtacagggta cttaacagta tcagccctta tacaaattat gggggaagaa
    23641 aaagcatggc agtatatgga taagttgcat gagaatattg gtgtatacac tcaatctggt
    23701 tcagctccag ctacatcagc agcttcagga gaatatccta ttggtatatc ttttggatat
    23761 agaggtataa aattaaaaga ggaaggctat ccagtagaag tagtatttcc aaaagaaggt
    23821 tctggatggg atatagaagc aaatgctctt gtgaaaaaag ataatataaa agaagcatca
    23881 aaagtatttt tagattgggc tataagtaaa gatgcaatga atgaatactc taagaactat
    23941 gcagttacta ctatttctac aggaaatcca ataccagaag gattccctaa aaagccatta
    24001 gagcaaatga tagataatga tttaaaatca gcagcaaaaa atagagaaga tatactaaat
    24061 aaatggatat caaaatatga tggtaagaca gaaaaagaga gttaagtaaa actatacaaa
    24121 tggagtaaaa agaaaaaatt tttttaagta gattttatta taataatata agttgagaat
    24181 aatataggtc aagaaaagtg aatattctat aaaaatgtat agagtaagta taccattcag
    24241 tttgaatggt atatttttta tttttagaga ttaatttaga aatagatata ctacagttta
    24301 aaatagtata ttttaat
//
LOCUS       DAMJVX010000041        23308 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMJVX010000041 DAMJVX010000000
VERSION     DAMJVX010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.043"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(122..382)
                     /locus_tag="PC407_001544"
     CDS             complement(122..382)
                     /locus_tag="PC407_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496996.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(645..998)
                     /locus_tag="PC407_001545"
     CDS             complement(645..998)
                     /locus_tag="PC407_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2496997.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQAKQDDDITNNFI
                     ANAEIYEMLLSQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGRKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(1015..6192)
                     /locus_tag="PC407_001546"
     CDS             complement(1015..6192)
                     /locus_tag="PC407_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome condensation regulator"
                     /protein_id="HDF2496998.1"
                     /translation="MKQNKLLQRGAYFNDKNILIDDFDKRYNDYDFVEFFTGISNSTF
                     GLKSDGNLYACGDNTGFQLGLGKDSSERRMFSKVKIDNVKYVSCGSKHSVAVTKDGFA
                     YGAGTSNVGQLGVIESTVYYEFTKLPIDDVKTVACGYDFTFVLKNDGTLYSAGLNSSG
                     QLGLGDTNNRATFTKVNIDSVKDVVTYNQSVFIIKMDGTAHACGLNSNGQLGINSTLN
                     KSVFNKIEGMDNVKQIACGSSHTILIKNDGTMYTTGYNGVGQLGTGNNNNSIVFTLSS
                     INNVKYASCGNNHTMILKYDNTLFSTGQNNYGQLANANKDVASRNTFAKVNVENIKDI
                     KCGSQFNFLINGSKEIFVSGCNLAGQLGSFFHTTFLYEFSNVQSSNLDNYSGLLVNDD
                     YLYVTKDNSEFLNVKLSDNFQDYKKIELTDSNMFIVMNDGTLYACGLNNYGQLGLGDT
                     VNRSVMTKVDIDNVLDIKGNGNSTFVLKNNGTLYSCGLNSNGQLGLRDEVNRNIFTKI
                     EIENVKDFCVGSNYVIALNHSKEVYGWGNNPYNNIEKTSNYPYKQGISNIEKIAAYDY
                     SVYMINSEGKLYVSGYNYNYQLGKGNNSNQSKALVSQCRTNSTSSTSNGLRTLPKITN
                     VFPFYDGCAIIDEGGYVYLTGYHGYLRTLNSSPSISDYSRYGTFIEATNSNHNTYFIQ
                     ETDFSGIEKVIGMSNNILFFKKGSSYITGYPKTFGSTITGHRSYTSINSESSNLGSNF
                     IIYHSNSKLYGKGIANSGQFGNSTNIDGTSNYDTGLKDIKDIIVKGNTVVVVDKNNNI
                     YVTGMNQNNKLGIGEYNNEPVKKFTNITEQSNSFIFMDDIKEITTSRNTMFIVKNDGT
                     AYATGNNSSGQLGLGDTINRNKFTQINLDNIKKISTSIDGNTTFAIRNDGTLYSTGLN
                     TKGQLGLGDIVNRNTFTKVNIQNVRDVVLGTTHSHAIKDDNTLYSCGENTHGQLGLGS
                     ESNHPDVLTFTVNNITNVRDVYCSDTTTFIVKDTNIAYCCGYNNNSQLGMGNTTDQYS
                     FIKCMENVKEVIPNEINTYIITIYNTAYSTGLNTDYCLGLNSNSNQSSFSEIPISNVV
                     KVAPNRNNAVLLLTSEGDVYTAGKCSNGSGTGSETPEKIKKIASKAKDIGMNYRCGHY
                     VSDNGDLYGTGFNNNGQLGVGDVTKRDTFIKTNTRVKKILPLEYANIAIKDTNDIYIC
                     GLNNYGQLGVGNRYDSRNNDNRIFNYKHMNFVMGDLTSIKNRHNFILLNNKIVIPTTK
                     DIDYGLVLGNLYKGDLYTELPYEDIKEVSISKTHIIILLNDGTMYGCGTNYHGELLQD
                     LSINQVDEFVQINVSDVKHVSCGDNFTYFIKSDDSLWSIGKNSEYQLGIGHNNPVTEL
                     QRITTISSCKEVHCGKNYTLVVTTGNELFVQGYNDKGALGLGSDSENTIIKFFTKALT
                     DIREIKSYGSDHILVLKNDNSVWVTGKNRDVYKIEQPVEFLKEFTIVPISEDVNTVKD
                     VLATDNTLYIISEVGTTNAAIEITEKSISSIKIKIQDPNKDISRIEMLINGESVKSVS
                     DLTTEKISFEVPPDKIKIGENKILFRAYCKGDDLYASLFIFKESTGNSIIKDSYVMIG
                     NRMYKVVNTTSNEQDITITLDRGLEEDLNLGDPIYQLINKTKVQVKINKSDLFKDMKL
                     VEIKKSDSSYQEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(6214..7200)
                     /locus_tag="PC407_001547"
     CDS             complement(6214..7200)
                     /locus_tag="PC407_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2496999.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGDTDILTTDSKDLSGAINEVVKKIENISFDDVISGQIQTDISVLKNSY
                     NKLSEKVLDILIYLELESEVTVDEAGYWYDTLANGNNIVAIEGLKLDLNRKCITGEIG
                     NVIFRDVVLPFSANRVRYIHDMDNNFVETKSSNTYLKEQKDITLSKYSYEIR"
     gene            complement(7201..7899)
                     /locus_tag="PC407_001548"
     CDS             complement(7201..7899)
                     /locus_tag="PC407_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDF2497000.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSEMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(7896..8948)
                     /locus_tag="PC407_001549"
     CDS             complement(7896..8948)
                     /locus_tag="PC407_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2497001.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGENNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(8941..9369)
                     /locus_tag="PC407_001550"
     CDS             complement(8941..9369)
                     /locus_tag="PC407_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2497002.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(9369..9695)
                     /locus_tag="PC407_001551"
     CDS             complement(9369..9695)
                     /locus_tag="PC407_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HDF2497003.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(9711..11240)
                     /locus_tag="PC407_001552"
     CDS             complement(9711..11240)
                     /locus_tag="PC407_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2497004.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(11522..11944)
                     /locus_tag="PC407_001553"
     CDS             complement(11522..11944)
                     /locus_tag="PC407_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497005.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEVATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(11944..14397)
                     /locus_tag="PC407_001554"
     CDS             complement(11944..14397)
                     /locus_tag="PC407_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497006.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASSRVNSLNSSLRKVQTTASSVSSTMAKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKRVEKRPYQGSINLKDMVSSAMARILPKLMLFKNT
                     FWSGVIAIKDMASSIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKTSQKDVDKYYDSLVNMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSSKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(14397..14564)
                     /locus_tag="PC407_001555"
     CDS             complement(14397..14564)
                     /locus_tag="PC407_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497007.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(14591..15037)
                     /locus_tag="PC407_001556"
     CDS             complement(14591..15037)
                     /locus_tag="PC407_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2497008.1"
                     /translation="MNENGLSKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(15151..15579)
                     /locus_tag="PC407_001557"
     CDS             complement(15151..15579)
                     /locus_tag="PC407_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2497009.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(15593..16657)
                     /locus_tag="PC407_001558"
     CDS             complement(15593..16657)
                     /locus_tag="PC407_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF2497010.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTEEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(16662..17105)
                     /locus_tag="PC407_001559"
     CDS             complement(16662..17105)
                     /locus_tag="PC407_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497011.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPIMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTITLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(17354..17794)
                     /locus_tag="PC407_001560"
     CDS             complement(17354..17794)
                     /locus_tag="PC407_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497012.1"
                     /translation="MENKKDILFKETDERLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(17899..18096)
                     /locus_tag="PC407_001561"
     CDS             complement(17899..18096)
                     /locus_tag="PC407_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2497013.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            18265..18585
                     /locus_tag="PC407_001562"
     CDS             18265..18585
                     /locus_tag="PC407_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2497014.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            18667..19173
                     /locus_tag="PC407_001563"
     CDS             18667..19173
                     /locus_tag="PC407_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497015.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            19874..20629
                     /locus_tag="PC407_001564"
     CDS             19874..20629
                     /locus_tag="PC407_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2497016.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(20772..21200)
                     /locus_tag="PC407_001565"
     CDS             complement(20772..21200)
                     /locus_tag="PC407_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2497017.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(21621..21821)
                     /locus_tag="PC407_001566"
     CDS             complement(21621..21821)
                     /locus_tag="PC407_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2497018.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(22004..23173)
                     /locus_tag="PC407_001567"
     CDS             complement(22004..23173)
                     /locus_tag="PC407_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDF2497019.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSEDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
BASE COUNT         7204 a         4160 c         2454 g         9490 t
ORIGIN      
        1 ttactttttt taattaatat tttaaaatat aaaatccttc tcaataaaat agggcactat
       61 ctacaatact actttatctt ttcctaataa tatataatct ggtgctactg ctactttcat
      121 gttatacacc gttttgtaac atacctttta tatcagtaac atcttcctta atttcctcta
      181 catctgtttt cattattccc atttgaacaa gtatattttt gtttatttct tgttgttgtg
      241 tagataattc tataaaattt tctacagttt ttttatacat gtctctatct tcttttttct
      301 cctgcatagt atttttaaat aatagagcac atagtatacc tattgccccc agactactca
      361 attcggttat taatttatcc attatagcct cctattgtct aaatttttta tacttatagc
      421 tttaattttt gttatattct tacatcatgc aatattatat tttcaggatt atcaatttaa
      481 tcttctgtac tttaaattgt aggtttaatt tatagattga ataaataata aatatatata
      541 caatttctaa tataacaaaa aataagtaca aggcttactt attaaagtta agaccttgta
      601 ctttacataa aaaaaataac taatacatag ttattctaaa tttactattc aactggtatt
      661 tctaattctt tacatctaat tctaacttgc tctctaatta ctgctggtac ttcttctatt
      721 gtttttctgc ctcttaaaat taaagctaca tatacttcta ccattccact ctcacctcca
      781 gattttctta cactaaaagt gcttatatta cttgcttcat tgacagaact ttgacttaac
      841 aacatctcat atatttctgc atttgctata aaattatttg ttatatcatc atcttgcttt
      901 gcttgaccta tacttatgtc tgaaatgtct ttatactgca tttctacttt tctaccttgt
      961 ttgatttgtt ctattttatt tttatcgaat ttaaacaatt tttacacctc cattttaagc
     1021 ttgtggctcg ttgttttcag catcatatct aaaaatcata gatggacgtt ttatagctgt
     1081 ccatttatct cctttttcta ctatgatttt aggctgagca ctttttatgt tggcttcttc
     1141 taattcatag atttcttgat aacttgagtc tgattttttg atttcaacta gtttcatgtc
     1201 tttgaataag tcagatttat ttatttttac ttgaacttta gttttattta ttaattgata
     1261 tatagggtct ccaagattta aatcttcttc aagtcctcta tctagtgtaa ttgtaatatc
     1321 ttgttcatta gatgttgtat taactacctt gtacattcta ttacctatca taacataaga
     1381 atctttaatt atagaatttc cagtactctc tttaaaaata aataaagatg catataaatc
     1441 atcaccttta caataagctc taaatagtat cttattctct cctattttaa ttttatctgg
     1501 tggtacttca aaggatattt tttcagtagt taaatcactt acagatttta cactttcacc
     1561 atttataagc atttctattc tacttatatc tttattaggg tcttgtattt ttatcttaat
     1621 tgatgaaatt gatttttcag taatttctat agcagcattt gtcgttccta cttctgatat
     1681 aatatataat gtattgtctg ttgcaagtac atcctttact gtatttacat cttcagaaat
     1741 aggtactata gtaaattctt ttaaaaattc tactggttgt tcaattttat atacatccct
     1801 attttttcca gtaacccata ctgaattatc atttttaagt actaatatat ggtcacttcc
     1861 ataagatttt atttctctta tgtctgttag tgcttttgta aagaacttaa ttatagtatt
     1921 ttcactatcg cttcctaatc ctaaagctcc cttatcatta tatccttgta caaataattc
     1981 attacctgta gttactacta atgtatagtt tttaccacaa tgtacttctt tacagctaga
     2041 tatagttgta attctttgta attcagtaac tggattattg tgacctatac ctaattgata
     2101 ttcggaattt ttaccaatag accaaagact atcatcagat tttataaaat aagtaaagtt
     2161 atctccacat gaaacatgct ttacatctga tacattaatc tgcacaaatt catccacttg
     2221 atttatagac aagtcttgca ataattctcc atggtagttt gtaccacatc catacattgt
     2281 tccatcatta agtaatataa taatatgagt cttagaaata gatacttctt ttatatcttc
     2341 atatggaagc tcagtataaa ggtctccttt gtataaattt cctaatacta aaccataatc
     2401 tatgtctttt gtggtaggta tcactatctt attgtttaga agtataaagt tatgtctgtt
     2461 tttaatagat gtcaaatcac ccattacaaa attcatatgc ttataattaa atattctatt
     2521 atcattattt ctactatcgt atctatttcc aacacctaat tgtccatagt tatttaatcc
     2581 acaaatatat atatcattag tatcttttat tgctatattt gcatattcta aaggaagtat
     2641 tttctttact cttgtattgg tttttataaa tgtatctctt tttgttacat caccaacacc
     2701 taattgtcca ttattattaa aacctgtacc atataggtct ccattatcac ttacataatg
     2761 tccacatcta taattcattc caatatcctt tgcctttgat gctatttttt taatcttctc
     2821 tggagtctca cttcctgtac ctgaaccatt actacatttg cctgcagtat atacatcccc
     2881 ttcacttgta agtaaaagta ctgcattatt tctgtttgga gctactttta ctacatttga
     2941 aattggaatt tcagaaaatg aactttgatt gctattacta tttagaccta agcaataatc
     3001 agtatttaaa cctgtactat atgcagtatt atagattgtt attatatagg tatttatttc
     3061 atttggtata acttctttta cattttccat acactttata aaactatact ggtcagtagt
     3121 atttcccata cctagttgtg aattattatt gtatccacaa caatatgcaa tgtttgtgtc
     3181 ctttacaata aatgttgttg tatctgagca gtacacatct cttacattag ttatattgtt
     3241 tacagtaaat gtcaatacgt ctggatggtt gctttcgctt cctaagccca gttgcccatg
     3301 agtgttttct ccacatgaat ataatgtgtt atcatctttg attgcatgcg agtgagtagt
     3361 ccctaaaaca acatctctta cattttggat gtttactttg gtaaatgtat ttctatttac
     3421 tatatcacct aatcccagtt gtcctttggt atttaatcct gtggagtata gtgttccatc
     3481 atttctaatt gcaaatgttg tgttaccatc tatacttgtt gatattttct ttatattatc
     3541 aaggtttatc tgagtgaact tatttctatt tattgtgtca cctaatccta attgtccaga
     3601 actattattt cctgtggcat aggctgttcc atcatttttt actataaaca ttgtatttct
     3661 tgatgttgta atttctttta tatcatccat aaatataaat gagtttgatt gttcagttat
     3721 atttgtgaat ttttttactg gttcgttgtt atattcccct atcccaagtt tgttattctg
     3781 attcattcct gttacatata tattgttatt tttatctact actactacag tatttccttt
     3841 tacaattata tcttttatgt cttttaatcc tgtatcatag ttacttgtgc catctatatt
     3901 tgttgaattc ccaaattgcc cactattagc aatccctttt ccatataact tggaattact
     3961 atgatatatt ataaaattac ttcctaaatt agaactctca gaattaatac tagtataact
     4021 tctatgtcca gtaatggttg agccaaatgt ttttggatat ccagtaatat atgaacttcc
     4081 tttcttaaaa aataatatat tatttgacat ccctattact ttttcaattc cactaaaatc
     4141 agtctcttgt ataaaataag tattatgatt tgaatttgta gcctcaataa aagttccata
     4201 tcttgaataa tcagatatac ttggactgct atttaatgtt cttaaatatc catgatatcc
     4261 tgttaaataa acataacctc cttcgtcaat tattgcacaa ccatcataaa aaggaaaaac
     4321 attagttatt ttaggtaacg ttctaagtcc atttgatgta gaagatgttg aatttgttct
     4381 acattgagat actaatgctt tgctttggtt actattattt cctttaccta attgataatt
     4441 ataattgtat ccagaaacat atagtttccc ttcactgttt atcatatata cagaataatc
     4501 atatgctgct atcttttcaa tattacttat tccctgctta tatggataat tagaagtttt
     4561 ttctatatta ttataaggat tatttcccca tccatatact tcttttgagt gatttaaagc
     4621 tatgacataa ttgcttccta cacaaaaatc ctttacattc tctatttcta tttttgtaaa
     4681 tatatttcta ttaacttcat ctcttaaacc caattgtcca ttactattta aaccacatga
     4741 atataatgtt ccattattct taagcacaaa agttgagttt ccgtttcctt ttatatccaa
     4801 aacattatct atatccacct tagtcataac tgacctgtta acagtatctc ccaatcctaa
     4861 ctgtccataa ttatttaaac cacaagcata caatgtacca tcattcataa caataaacat
     4921 attgctatct gttaactcta tcttcttata atcttgaaaa ttatcactta actttacatt
     4981 taaaaattca ctattgtcct ttgtaacata taaataatca tcattaacca ataaacctga
     5041 ataattatct aaatttgaag attgcacatt tgaaaactca tacagaaaag ttgtatgaaa
     5101 aaatgaacca agttgacctg ctaaattaca gccagataca aatatctctt ttgaaccatt
     5161 tattaaaaaa ttaaattgag aaccacattt aatatctttt atattttcta cattaacctt
     5221 agcaaaagta tttcttgatg ctacatcttt attggcattg gctagttgac cataattgtt
     5281 ttgtcctgta ctaaacagtg tattatcgta ttttaatatc atagtatgat tatttccaca
     5341 agaagcatac ttaacattat ttatactaga aagagtaaat acaattgaat tattattatt
     5401 tcctgtacca agctgaccaa ctccattata gcctgtagta tacatagttc catcattctt
     5461 aataagaatt gtatgactac taccacacgc tatctgtttt acattatcca taccttctat
     5521 tttattaaat acacttttat ttaaagtact attaattccc aactgcccat ttgaatttaa
     5581 tccacatgca tgtgctgtcc catccatttt tatgataaat acagattgat tataagtcac
     5641 tacatctttc acactatcta tatttacttt agtaaaagta gctctattgt tagtatcacc
     5701 tagtccaagt tgaccacttg agtttaaacc tgctgaatat aatgttccat catttttaag
     5761 cacaaatgta aagtcataac cacatgcaac agtttttaca tcatctattg gtagcttagt
     5821 aaattcataa tatactgtag actcaattac acctaattga cctacattac ttgttcctgc
     5881 tccatatgca aatccatctt tagttactgc tacactgtgt tttgaaccac aagatacata
     5941 ttttacatta tcaattttta ctttactaaa catccttctc tctgacgaat cttttccaag
     6001 tcctagttga aaacctgtat tatcgccaca agcatataaa ttaccatctg attttaaacc
     6061 aaaggtacta ttacttatac cagtaaaaaa ttctacaaaa tcataatcat tatatctttt
     6121 atcaaaatca tcaatcaata tgttcttatc attaaaataa gcaccacgct gtaaaagttt
     6181 attttgcttc attattagta ctacctcctt tatttatctt atttcatatg aatatttact
     6241 tagagttata tctttttgtt cttttaaata agtgttacta gattttgtct caacaaagtt
     6301 attatccata tcatgtatat atctaactct atttgcacta aatggtaata ctacatctct
     6361 aaaaatcaca ttaccaattt cacctgttat acattttcta tttaaatcta acttaagccc
     6421 ttctatagct actatgttat ttccatttgc taatgtatca taccaataac cagcctcatc
     6481 tacagttact tctgactcta attctaggta tattaatata tctagcactt tttcagataa
     6541 tttgttatag ctatttttta atactgatat atcagtttgt atttgaccac ttataacatc
     6601 atcaaaagat atattttcta tttttttaac tacctcattt atagctcctg ataaatcttt
     6661 agaatctgta gttaatatat ctgtatctcc aatttttact tttatttctt ttatattttt
     6721 atcaaactta tcatctaaat cttgaatatc cttttgagtt acaaaaacca acgtcgggtc
     6781 tacttctaat gtaacatttg aagtattgga caattgtaaa atcattttta caaccaaatc
     6841 tttaatactg ccatcttcag cacgtggctt atacgtctct ggatacttac ctatagctat
     6901 tatattattc tcattatcca gaacagcagc ttctcttatc ataaatccac caactgttcc
     6961 tggtaaaata gtctctataa ctatccaatt aggatttttt tcatcaattc ttagagaatt
     7021 tatatttcct tcccaaacta cattctttaa agcagtttgt tcctctgttg gctcataata
     7081 ttctccatta ctatctccaa cttgaaactt ggttaatcca acaagctctc ctagtgcagt
     7141 tgcatttgct attttttctc taccaatttt ggttaatatt atataaaatt tttcttccaa
     7201 ttataccacc tccgatttag gataaactac tacattttct acgcctgtat catttgcaat
     7261 agctatatta atcttacaat tattttcaat gcatttaatg cttaaaggat aaacctttat
     7321 ttcttctcta cttaacattc cagctccaca gtttacaaca gcactacatt ttataattgg
     7381 ttcaaagtat ggatgaactt ttacctcttc actacatacc atacctccac cacaatagac
     7441 catactttta tctagtatta ttggttctaa atagtgtaat aagtgagcag gttttactct
     7501 ttcaatcatc tcgctacaat ctaaaagagt tttatagtca cagttatttg ctataaaact
     7561 caatacgaat gtaaattcat cactataaac ttttatatct gtttctgatt ttgtatatgc
     7621 ctcacatata ctttttataa cttcaatagt actagtacct ctgcttctca ttttagcttt
     7681 tatattgcta cgtcttgtgt caaaatcaag ttcttttttt tcaatgcaga gtatgtcttc
     7741 ccacatatct aatccccatg tcgctgtatc aacaaataat tggttaaagg tatcatcaat
     7801 atcttcttta agaatattta attctgtatc gtatgcacct tgtatctcct ctacaatgta
     7861 atttctatca aatgatggta gtttatcaat tagcttcatt gattttccac ctcactaaaa
     7921 ttaacagttg ttacactagg tattttatct tcattaatag ttatattatc tgtacttcca
     7981 tttataagta gattacttaa atcgtgtaca ccagtagtat ttataagtat tcccatgact
     8041 tttgtataga ttatctctcc tctaatatct ctaaagtatg tatttatact ttctaggaaa
     8101 gattctttta cattgtctaa tgtatatcca tcttctagtt tcattactgc acttatactt
     8161 atttctattg gtaatggtgt cacaactgtt atagttggtc caataggctt ctcttcatct
     8221 atatgttgct gacacctttc aatcgtttct gtatcaacag cttgattatt ttccccaaat
     8281 atcaagacct taactgttcc tggaccatcc catcttggat aaaccttaac attatagact
     8341 ccatctactt ccaaagccca ttcttcatag tgagctttat ttccacttgt agcttgattt
     8401 ctttggattt tataaaatct ttcttttagt tcttcatctg tctctatatc tgtaccacct
     8461 tcaaaacttc ttgtgttagt aatctttgtt actccagata tattatcaac tagtttaaat
     8521 tcacaatttg taggtaaatt atatttctta ccaacttcca gagcttgaac tgggctattg
     8581 tcaccttctt cactaccaat agttacatct tttattacta caaatagtaa atctctatat
     8641 gatattattg tgccattatt tattacagtt cctttctctc caataaattc aacttctcca
     8701 tttgactctg tacctaactt tctatataca ccaaattcat tgactctttt atcaagaaac
     8761 tggttatatg tgtcttgtat aaaagccatt ttatgtatct ttgaaagttc tagatatatc
     8821 ttcgaaagtt ctagattatt tccagatacc atgttgttta gaaaagaacc ttctccttta
     8881 taaatatcaa gattaatatt ttcaagagtt ctatttttta ttacttcata tgtctggtca
     8941 ctatacattt atttccacct ccccatagat agtggatact tttacatttg cactcaatac
     9001 gtctctgtta aagttagcac tttttacgtt tacttctaat atatatggat ttattagaag
     9061 ggcctctttt atgaatctac tagcttcact ttctgtaaga ccttttgtat atttttgtcc
     9121 tattagttct gaaagctctg tcccatattc taaagagtat atctcatgtt catatctatt
     9181 tgtctttata cacttgtata tccaaacttt taaggcttct tttttttcaa ttattttaaa
     9241 gtcacctttt tctaaaatag gttcatcttt ttcaaaatcc catgccactt cacgaaagat
     9301 tggcaattct tctgttttag gtaagatata atcctctggg acacctataa aaggaaatat
     9361 tgtactcatt ataagctcac caacttactt acaacagcaa atttttcgcc tattctaaac
     9421 attattacca tatccccaga ttcaaagtta tctataaaag gattttttac ttcatgctgg
     9481 tgttcttgat ttgtttctgt gtcaaatgtt tctagctgtc tatcaatcat ccaactatct
     9541 atcaaaatat cttctttttc taatatgatg ttatttgtct ctatttttaa atctggtaat
     9601 ttacttttaa tttttccaat aaaaaaagaa ggttgattat gatactttcc ttcttctctt
     9661 attattccta taaattcatt tattggatta gccattatat caccatcctt ttataatact
     9721 ctcctagctg tattaaaatc tcttctacta cttaacttac ttattttaac tacatcacct
     9781 ttttgtggag catgtaaaaa ttctccatta cctatatata atccaacatg gctgactgga
     9841 ttatgaaaaa atactaaatc tcctgcttga agattttttt gttctacttt cttgcctttt
     9901 ttagattgtt gatttgacgt tcttggcaaa ctaacattaa cttttttaaa acagtagacc
     9961 attaaaccag aacagtcaaa actacttggt ccatttccac cccatttata aggtttacct
    10021 agatgttttt ttgcctctga tattactatt ttagctttat cagatacatt accagaataa
    10081 ccaccaataa taatttttcc ttttcttctt ccaaatttac tagcttcttc aacatttcca
    10141 aataaaatat ctatatggta tgttccattt ttttctatag ttattgcagg tcctacgtca
    10201 tttactttat agactccatc acgaccagaa actcctgtaa ttacctgtat ttcatctcca
    10261 tagttcaata aaggatgttt atttaaaaac tcttttgtat aataagtttg ctcatattta
    10321 ccaaccatag gagcagcgca agttttttta gatgggtcaa gttttttctt tctacaatct
    10381 gtatctccac cttcttcttt tctaggacag taagctgtaa attctgctgt aaactctttt
    10441 cctcctgcat aatcttctcc ccctaaatta ctttcttcct taggttcatc ctgtcctgct
    10501 gacttttcat ccataagatt ttgaaaatta agttcaagct caatttgata ttctccattt
    10561 tgccaagtat gtttgtctgt atctatataa aatagtccta caagctttgt ataagaatct
    10621 ttaactttta ctcctctacc agttatacaa cttacatctc cataaccttt aagagaacag
    10681 cttttttcta tcccattaaa ctcgctatca atatctacat cttgattttc ttgttgctga
    10741 attacttttt gcattattac atttacttcc ttaaaaattt cattatctat tttttcgcta
    10801 atcttgcttc catactggtc taccactatt actttatttt ttacattttc catgctctcc
    10861 gaaaaggtgg tatttataat attaaatccc tcttcaaaca taacacttaa tgtaacagtt
    10921 cctttttcaa taacattaaa cttatctaaa ttagcctcta tcatatactt tttctttgtc
    10981 tttttacttg cctctgtata tgcactcatt attgtgtcat aaccatttac tcctataaac
    11041 atctttgtat acttgacatt ggtctttgct attgttccaa ctgaaagcct attttcagca
    11101 aatacttgct ttgctatgtc ttcaactaac ttatctttaa aattgtatga cacttcactt
    11161 tgtgtaagta aaaatcccat atctttagat acaaaactaa tttcattact gcttgaatct
    11221 ttagacctat taattatcat tcctctaaag agttctttct catctacata gaaacagact
    11281 gtactagcta ttggtatatc gatttgttgg aaatttacat caaatgatga ttgtattatt
    11341 gaaaactcta gtgtccttga tggagattta taatcacctg accatgatac cttgtctact
    11401 atgtcagtta tgtcatatat acttccgttt tttatgtgta cccaaatctt aataataaaa
    11461 aatcacctgc cttttcattt aattctatca gcagttactt acaacttatt atctataata
    11521 gttattgttt tggagttact tttggtatct ttattctttt atactctttt aaatctaatg
    11581 taaaatatac atctcgagtc ccatctcttt cttcataact aaacccttca attatgactt
    11641 ccatatttat atttgtttcc gtaattgtaa atcttaatat aaaaccttcc ttcatccatt
    11701 tttctatctt atttacacaa tcatatggtg atggaaaacc tgtatagtca cagaaagtgt
    11761 agtctttatt tggaaaaaaa cttgatattg aaatgctttt aagagctaca ccaccaaaag
    11821 ttgctacttc acctattttg agtatactag aagagtttac agcagctttt ccattaatac
    11881 taaaggaaga tggaaatact ggaaatctaa atctatcttc tgcttgtcta agccacattt
    11941 ccattataag acacccccta actgtgccaa ttggatactt gcaactaatt ttgatgttat
    12001 cttttctata tcagcttctt ctctaatcac tattgtatca gctaacttag ctatgtttat
    12061 atttccacca ttttttctgc ttctatattg atttgcttct tgtttagtta aaaccatttc
    12121 accttcatgg agtctggttt gatagtcatt ataaggaaca tagttcaatc caaatgcatt
    12181 attacctttc ttacttgaat aagctttacc agaatcacca gcctttgttc cagcatatgg
    12241 attatctttg taattttttt tactcttttc cactagattt aaaactgttc ctgtgatgct
    12301 tccatttttt atcttattaa tcatagtttg ccaccatttg catatatctt taactatttt
    12361 acatacttta tctaatatgt taaaaaaagc tcccaatatt ggctctataa ttttccatcc
    12421 agtttccaac agaggtccta aggtctccca tacagttttc caaataacac ccagtgtctc
    12481 tataaaattg tttatttgag gtgcatattc acctaaaaaa gtaaaaattc ttgtgataat
    12541 aggactaaca gcttcaacta caggtccgat tagttggaat gctgtagtta ctattgtacc
    12601 aaatatactt attaatcctt gtgcaattgg tgatttattg tttatactat ctagtaaatt
    12661 atttactaag tttactactg gaagtagtga atctattaat gctgtaaaat caatattttg
    12721 taatagtgta cttcctgcgt taacaattgc ttttggtgca ttccctatca ttgttgctgc
    12781 atgattttca aaacgttctt gcttattttc aaccatcatt ttacgctttt cagcctcatt
    12841 agaaattcga tattgagtag aatcagaaaa ctcttttcca ttttttacac ttctagattg
    12901 catctggtca tatttgttgc caataattat cttaccattc attacattgc ctattttacc
    12961 agccattttt tcagataagc cagctaaatt ccctaatcca ctttctataa aagtttttat
    13021 tttctttagt ccttgaccta aaatatcatc aaaaggttta acaatactct tcaaaccatt
    13081 gttaatgctt gtttgagctc ctgatattat acctggtatt gtattactca tttctttagc
    13141 taaccccatc tgctttacac ttatgccttc caatgcttca tcaaaagttt tataattttc
    13201 tcctaccaga gtattaagag attccatatt tccttttgct gcacttaaga aagctgctga
    13261 tacatcttgt tcactacttg tattcatatt taaagctcta acatctacca tagcttgagt
    13321 tatatctttt tcttttttgc caccagtaat attactaatc attttagctt tagttcccat
    13381 tgcaacaaca tcttcagggt caaaaggcgt atcatttgcc atatttacta aactgtcata
    13441 atacttatca acatcttttt ggctagtttt tccagtattt cttttcatta cactttctag
    13501 aacaactttc tgattttgtt cctgaccaag taagtcaaca ccctttttca cagcgacacc
    13561 tatagtagca ccatttgtca aatcagatat cttccctttt atcgaaccaa gtattccact
    13621 tgccatatcc tttacagcta ttgcaccact ccatacctta cctgcaaaca atctcaattt
    13681 gggaaatact ttacttataa tgctacttgc catatctttt atagctatta caccactcca
    13741 aaaagtattt ttaaacaaca ttaacttagg caaaattcta gccatagcac tacttaccat
    13801 atctttaaga ttaatacttc cttgataagg acgtttctct actcgtttta attttcccaa
    13861 gatactactt atcactctct tagtcatatc ttttgctttt attacgatat tccaaatctt
    13921 gccaccaatc ttttttaaat ttccaagtaa tttattttta gttttttcta cttcatcact
    13981 tttagcttta aatattaata taatcataat atttgaatta aactcatttt tcttttgaat
    14041 caacttatta aaataatcaa taatcatagc tatagttatt gctagtctca taatacttcc
    14101 atttaaactt ccaataactc ctgaaacagc atttatgcta gaacttaatt ttgccatagt
    14161 tgaacttaca ctactagcag tagtttgaac ttttcttaac gaggaattta aactgtttac
    14221 tctactactt gcacttccaa tagaattttt tatgctacta gccattacat tagtggaaag
    14281 tgaaacagcc acagttgctc tcctaaaatt atcagttact gatataattt ggttcatttt
    14341 tggtgtgtaa ttatctgtaa gatacatatt tataccttct tctctcacat ttcccattac
    14401 ataccacctc ctgtcaaagg acacactcca tatccatgtt tcattttttc aacttcttcc
    14461 tcaatttcta aggcgaaaaa agcttcaata agctttaatt cgcctttatc cattgcataa
    14521 aaaagggacg gtcttatacc ttttttcttc caataatagt acattattgt agtcttggta
    14581 tccgtcccta ttagtttttt acctcttcta tcactgcatt tttaccataa cccataacat
    14641 cttgaagtat tctatatagg ttggtaattt cacctggtaa taatagtttt cttgccaatt
    14701 cttttggtgt tggagcctta aatttatcca ttagttcctt atttttaaat aaaagctttc
    14761 catccaaatc acaaacacct tctataattg taagcatctg catctccggt aagtctatat
    14821 ccgttttcat attttcatca attttaagac aagtattttg tatctcttca tctttactta
    14881 ttgttaaagc tctacacatt actttaaatt cctgcccaaa tatagttgat aatctcttaa
    14941 gttcaactat agtacttggt ctttctaagt tttctgtatc tgcatttaaa agtaaatcta
    15001 ctatgtttat attttttgat aatccatttt cattcattat tatttgctcc tttatcaatc
    15061 aaattcaatc aactaacttt tttacttatt aactacctaa atttattaag tatatatcta
    15121 ctaaatatca gtataaaacc aaatataaaa ctaaattata tcaagaaaat catactcagt
    15181 aaatgtgaaa ggtgcttcta tctctccttt tactccaacc tcccaatcag ctaaagttaa
    15241 atcatcaaat gctatatttt ttactgctat tctttctgct ccataagaat ctgggtcatt
    15301 taatttagat atagcaacaa atctaggttc agaacctctc tttatctttt ctccaattaa
    15361 cttgtgcatt cttgaactaa catggtatag agttattgaa ccttttcctt tatatcccat
    15421 atactttgta tcagtaccca tttgacctgc tattataata tcctcttttg taaattccat
    15481 ctttgcttga aacttcttta cttctgctac tttgtttcca tcaagccaaa gttctcccca
    15541 agtaccactc attacatttc tagcttccat atttgccata ttaataatcc tcctatattt
    15601 ctattgataa atctatatct tccatagcat caagtacttt tatttttgct tttaaaaata
    15661 ctttagaacc tgtgttagct tcttttattt cttgtaatgt catataagat aaatctactc
    15721 cttttgattt taaatacgat ttttgtgctt caaaatctat ttcaacagta gaatcagatt
    15781 ctataagtgc tgacttttct aattcttcta aataactttt tatagctact atcaataaac
    15841 atttgttgtc ataactgtta gtaacctttc ctatatagtc atctattatc acctttctta
    15901 tgtcactatg tataatatct aaagtgtcaa ctatttttat tttctggaac atttctcctt
    15961 tttcttctgt taactcagtt aaagaattta ctcctcttgc aattcttata gcccctgctt
    16021 ccttaataag tataagctct cctttattaa cccttgattc tgcatcaact ttcgtcatct
    16081 taggtatatc aactacatca ctaagcttag tataagttac tgattgactt aaaggtgttc
    16141 ctgctataag tccagccacc ctacttgtaa actcatcaac actgtatttc ttttctccaa
    16201 ctaacacatc ttctgtagta aaattaatta tcccttcatg atttcctaca acttttccta
    16261 atacagcttt aaccttaacc ttatctatat ctctaagttt aattatccaa ttttttatag
    16321 cagtcttatc agcttctact gcttttggca tacatagata attaaattcc ttagtctcta
    16381 aaaaatctaa tgcagtttga atatctgctt ctccttctat tacataaact aataatttat
    16441 ttggagtgtt aacatttccc atcaaagcta aatttatata ctctttattt tcagcagata
    16501 aatcaactgg tatatcctct ttttcatgta tttcattaag acctagtgcc ttagcatctt
    16561 taagcaccat tgcaattatt cctctagctg aacgttcttt aacagttgta gctagctcct
    16621 taaatgatat gttgatactt ggtaatccta tagccatact atcactctcc taaatttaaa
    16681 ttgatttctt ccatattttc atatactaca tcttcttcat atacactatc cagataattt
    16741 agtgtaattg taaagttcaa tacataaatt gactcttctt ttttaatact ttgttctagc
    16801 tttgttatat ttaattttct gtctgttacc tttatatttc tgttgaataa cttatttaat
    16861 tcatctgaaa tttcatacat tctaatactc tttgactgtg gttttggtaa gtattttata
    16921 gagataacaa tatctttatt attcgtattt aacattatag gtgtacaaat aactgatagt
    16981 atacttacaa aaaaacaaga cccttcagag ccttgcacac tttcatcatc aataaaaata
    17041 ctttcagtaa agtttttttt gagaatctca ataattgttt ctaatatttc cttgtattta
    17101 agcaaataat caccacacta tcactaaata ttcttgctta atctgataat cttactataa
    17161 atgatataag tcatttttaa atatacatct catttattcc tgtcaaatat cttctatatt
    17221 tttaggtaat attattcttt tcaatttatc acctccttca atatggattt ctttcacaat
    17281 aacattatct cacatatttt attgcctagt gttgcaatag tgtatataaa gtgttgtaaa
    17341 gttgtcatat tttttataat atctcactca atttaaatat catttttttc tttaaattgt
    17401 agcaagatgt tctatctaag tgtagtttca tagaaatata tgtcatattg ttttttgtct
    17461 tactattata aaaaagatta aaaaattcca tttctatatc atttagacat gttagagcat
    17521 tttctatctt ctctttttct atttctatat cttctttttc catttttaat tttcttaatc
    17581 tttcctctct ttttaacacc tcattttcca cagaagaact tatgttatat gtgttactag
    17641 tcttttctgt atataccatt gctccacatc cagagtattc attctcacat ctttttatgt
    17701 ccaaattaat attctttatc tttatatcca aatacttata attatgtaat ctttcatctg
    17761 tttctttaaa taatatatct tttttgtttt ccatagtact tctccttttt tatatactta
    17821 ttattagctt caaatttaca ataatctctt tagtgcacag ttttttacta atctcacaac
    17881 tttcaagaac atattgttct ataactgtat aaaaaaaact tcttctatat tttgttctaa
    17941 aatactagaa atcttcttag ccataagaag accaggattt tttatacctc tttctatttg
    18001 agatatatag tcatttgtaa ttccagcctt tctggcaagt tgaatttgtg tcataccttt
    18061 gcattttcta taatatttca agttattttt aaacattgtt ttatacctcc attaccatta
    18121 agaatatttt gttcttccac acttagatta tatagaatca tttgttcttt atcaatagat
    18181 tttagaaaaa aatattcttt tatattttat tagttttatt ttttagaaca atttgttata
    18241 atctatacat aaggtggtgt tataatgttt gccaaaagat taagagaact tagaaaggaa
    18301 tttggattga ctcaaagaga acttggagaa aaagtaggcg tttcccaaag agtcttaggg
    18361 tactatgaaa cagaaaacag atttcctgat gaacacattt taaacaaatt ggctgatgta
    18421 tttaatgtat cagttgatta ccttcttgga agaaccttag ttaaggaaaa tattgataca
    18481 gtagctgcac atagaaaaaa tccacatgaa gagttacctg aagaagcaca agaacaactt
    18541 aatgattata tagaattttt actaaataaa tataaaaaaa aataaaccat atgatgagca
    18601 gtctattttt ctgctctttt aatataattt aaaggcaaac atacattcta ttttaggggg
    18661 atttttatga ataatttgga taagcttttt gaattagctt ctcaagaaga gataataatt
    18721 cattatacca cttatattgc aggtgattta gaagggctat atataaacaa acatggtatt
    18781 aaaattatat cattactcag taatttaaaa caaaactcaa aaaaattgac atccatctta
    18841 gctgaagaac ttggacatca tttcactagc cttggatact atgtatcctc ttataacgat
    18901 tattatacaa aaattattat agataagtgc gaaaataaag ccttaaaatg ggcttgtgag
    18961 ttcttaatta ctgaagaaga tataataaac ataattaatt ctggcatcac ttgcgtttat
    19021 gaaatggcag acatacttaa tgtggatatt actttctttc aaaaaagatt agaattttta
    19081 tcattaaaaa aacagtcttt acaacttgga aataataaat atttaatatt aactaatttg
    19141 ccgtatttct acatatttga tccaatttca taataaaaat agtagtaatt ttaactggct
    19201 taacagtaaa atctatatat ttattttaac aaattttggt ttctataaat tcaatattaa
    19261 tatataataa tgatatcata ttatttaaaa ttctaaaaac aaatatatgc ttttgttaag
    19321 aaaggttgtt tgatgaatgt ataaagaaaa gttgtttgat aattatttta agttcttagc
    19381 actattattt tggccaatta tgtggtataa gtggatagta atttcaaatg gtactcttga
    19441 aaatatgtta tttaccatat atgcaatcgt cgcaattatt ttcatcattt tatattcggt
    19501 gtttatgata aaatataaag acattacaca aatagatttc ttttacagga taagtactct
    19561 acttgctttt atatttacac tttttagctt tttaatatac ccaaagagtt tatttttctt
    19621 gtatttaaag attattttta ctggaatata cctttattat tcaatagtaa aaactcttaa
    19681 atttaaagat gatgaaggtg tagtgggtat tatgagctca cttttattaa ttgtaataac
    19741 tttgttctac taaatttaat tctatttgac ttaatatggc tttaaatatt agacatctac
    19801 attattttct gatattattt aagatggtag tcttatatta ataatgaaat acactatgaa
    19861 ggagttaact attatggaaa aaaaagtttt agcaacagtt ggagaaaaag agataactaa
    19921 tattgatatt gaaaatgcat taaaatcatt agacccttat caagctatgc aatttaaaac
    19981 tgaagaagga aaaaaacact tattaaatga tttagtgaat caagaattat tcttcttaga
    20041 tgctaaagaa gaaaaattag atgaagaaga aatatttaaa ttagaaatga aaaagataga
    20101 agaaaatgta ttaaagcaat ttgctataaa taaagtttta tctagtgtta atgtaacaga
    20161 agatgaaaaa gtaaaattct ttgaagctaa taagagctct ttctctaaac cagaaagtgc
    20221 aactgctaaa catatattag tagatagtga tgaaaaagct aaagaaatac ttgctcaaat
    20281 aaaatcagaa gaaatatctt ttgaagatgc agctcttaaa cattcttctt gcccatctaa
    20341 agatatgggt ggtgatttag gaacattcgg tagaggacaa atggttcctg aatttgaaga
    20401 agctgtgttc tctatggcta aaggtgaagt aagtgaacca gttaaaactc aatttggata
    20461 ccatataata aaattagaag accttcaaga aagtacagag tctacatttg atgaagttaa
    20521 ggctgaagtt gaaaaaagtt tattatatca aaaacaaaat gaagtttatg gaaataagat
    20581 aaatgctctt aatgctaagt acggaaacct tgtaaaatac aacgattaat cataaggtca
    20641 tatataaaaa agctgtcttt tcacaatatt tattagaaaa gacagctttt tatattaaag
    20701 tttaaatatt cagtttcata ttatcattct tttattgtaa agaactcagc ttaaaagttt
    20761 aaataagatt tttaattatc attagctaaa tttttatatc cacgtattat ctcataacag
    20821 taacctatta catctcttct ttttattatc ccaataaatg tgtcttggtc atctataaca
    20881 ggaatgaaat tttgatttaa agattttata actaaatctt caatatctgc gttgatggaa
    20941 acaggagaat tatccatttt tctatttata tctgtcactg gaacatcttc caatcccttt
    21001 aagtccagat ttaaatcgtt ttttaatgtc cataaaaaat caccttctgt tatagttcca
    21061 acatatttac catccttact tattattgga attgcagagt atttgtgata ttccattttt
    21121 tctagtgctt gccttatagt gtagtcttca tatatatatg ctacttcact ttttggtgtt
    21181 agaaagaata atatgttcat tacactaccc accgttactg aaaacttata aatattactc
    21241 aatatttata atgttacttt tttgcagtaa ctttctcctt tcttattttc taagtattct
    21301 taatactata cgctgtaaca agcgtttttc ctttgctcat tttaatctta ttatgcttta
    21361 ttaatatttt tatatggtct atcagtatca cttattctct ttttagaaaa tttactaaaa
    21421 attaattgac ttaaacttag ccataataat tttactctac actattaatt ttaacatatt
    21481 tatctcatta ttaatgattt tattttaata attttaattt attttgctat taattttatt
    21541 tacaaaaact gaaaaaaccg ctaaatgatt agcggcttat ctctcatatt actagaacac
    21601 taatatatat gaatttttaa ttataatcta actacgttag cagcttgagg acctttagct
    21661 ccatctacta cttcgaactc aacgctttgt ccttcttcta atgttttgta tccatctcca
    21721 gttatagctg agaaatgtac gaaaacatct ccttcacctt ctacagatat gaatccaaat
    21781 cctttttcgt tgttaaacca tttaactaca ccagttttca ttaataaatc ctcctaaaaa
    21841 aacacaaata tttagctact cttttctgta accttattac tactctatca tatatagcta
    21901 tgtttgtcaa ttattattat ttttatacat taataatcaa tttttttact ttataatttt
    21961 aaatgattat tatcttttac actttatttt tctttataat tttttaataa tttattttta
    22021 ttatatggaa tcttatggac ttatcactcc agcctaaatc ccatggcacc aacattctat
    22081 cagtattatg gcaagttacc aaaggatatc ctcttgagtc taatcctgtc actgtcgaaa
    22141 catgtgttat tctgcctttc ttctcataac caacaaaatc ccccggacgt aaattataag
    22201 cttctttgta tacttcttca taggaacctt tagcaattaa tgaacctctt ccactataca
    22261 gtgcataatt tttaaaagct tgtgcattta cccatgcttt tgtacctgct ctctcgctat
    22321 aattccaaat tgagttcttt ttgaaccttc cactttcaaa cattatctgt gatgcaaagt
    22381 ttgcacaatc tccaccttct ggattataat ccctatagtt tggattaaat ttcataccat
    22441 gttcttcatc agctgcggct ccacagtatt tatgtgcata atctatagcc tttttttgtt
    22501 catctgtaag ctgtaactct acaccatctt gttgcccaat atagtttctt atatcctcag
    22561 attttatatt ttctaaattt aaagagtctg caaaagggtc tgtgtaccac tctttagtta
    22621 tgacatattg attatctata atttttacat ttagataatg atatgtacct attctaaagg
    22681 cattttcttt gtccttttga ttttcatatg aatacttata ttcagtcgat actgaacaga
    22741 ttataccata gaggtctttt tctttttctt ttattttttt aatcttaacc cttgtcttta
    22801 tgtctttaaa ttttacacct tgcttactag accagttttc taaatacttc atctttttta
    22861 cttcattttc ataagcccac agaccaaatt ttttatccgt ctcataaaga ccttttaaaa
    22921 tgttttcatt tttctctaat attgccttat ttctgtagtc aaataagttt tcaagcaata
    22981 gtttatattg gtcttttaca tcatcacctt caacatttgc agattccatg actttttctt
    23041 ccatattttt catacttaaa tcattgatta aaaatacacc tgctgtagta gctaaagaca
    23101 aaaatacaaa tgccattata cctttgatgt acttattctt atttttcagt attttcaaag
    23161 ctacacctcc caaatagtac accttataaa tgaaattaca aagaagattc tgtcatatac
    23221 aataattttc atatttaata attctctatt ttattatgtc caatattaaa aattaattgt
    23281 atcaatacac tgtttttttt acaatttt
//
LOCUS       DAMJVX010000042        23178 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMJVX010000042 DAMJVX010000000
VERSION     DAMJVX010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23178
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.044"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(180..548)
                     /locus_tag="PC407_001568"
     CDS             complement(180..548)
                     /locus_tag="PC407_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2497020.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
     gene            complement(1076..1498)
                     /locus_tag="PC407_001569"
     CDS             complement(1076..1498)
                     /locus_tag="PC407_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDF2497021.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            1976..4879
                     /locus_tag="PC407_001570"
     CDS             1976..4879
                     /locus_tag="PC407_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2497022.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILVL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     ISIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            4912..5358
                     /locus_tag="PC407_001571"
     CDS             4912..5358
                     /locus_tag="PC407_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2497023.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            5481..5783
                     /locus_tag="PC407_001572"
     CDS             5481..5783
                     /locus_tag="PC407_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2497024.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            5964..7370
                     /locus_tag="PC407_001573"
     CDS             5964..7370
                     /locus_tag="PC407_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2497025.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFSDPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVISMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            7714..8772
                     /locus_tag="PC407_001574"
     CDS             7714..8772
                     /locus_tag="PC407_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2497026.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYASPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            8847..9533
                     /gene="araD"
                     /locus_tag="PC407_001575"
     CDS             8847..9533
                     /gene="araD"
                     /locus_tag="PC407_001575"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDF2497027.1"
                     /translation="MLEILKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNVVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            9551..10198
                     /locus_tag="PC407_001576"
     CDS             9551..10198
                     /locus_tag="PC407_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2497028.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GIGREELNVEHICVLDLNGNVIEVEKGIKPSSEVLMHIEIYKIRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFESWKYPEEIKF"
     gene            complement(10371..11738)
                     /locus_tag="PC407_001577"
     CDS             complement(10371..11738)
                     /locus_tag="PC407_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDF2497029.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLRSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(11928..12016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            12444..14564
                     /locus_tag="PC407_001578"
     CDS             12444..14564
                     /locus_tag="PC407_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDF2497030.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESILKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNKLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKEIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            14668..15921
                     /locus_tag="PC407_001579"
     CDS             14668..15921
                     /locus_tag="PC407_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HDF2497031.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFEDIETYSKDSQKKREKEL
                     DKFINTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEIYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTQV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGFMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(16075..17136)
                     /gene="ytvI"
                     /locus_tag="PC407_001580"
     CDS             complement(16075..17136)
                     /gene="ytvI"
                     /locus_tag="PC407_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF2497032.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLSLILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFLELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFIGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            17410..18150
                     /locus_tag="PC407_001581"
     CDS             17410..18150
                     /locus_tag="PC407_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDF2497033.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            18162..19940
                     /locus_tag="PC407_001582"
     CDS             18162..19940
                     /locus_tag="PC407_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2497034.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRILEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            20107..21024
                     /gene="pfkB"
                     /locus_tag="PC407_001583"
     CDS             20107..21024
                     /gene="pfkB"
                     /locus_tag="PC407_001583"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2497035.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYAKRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            21061..22980
                     /locus_tag="PC407_001584"
     CDS             21061..22980
                     /locus_tag="PC407_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2497036.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         9230 a         2519 c         3926 g         7503 t
ORIGIN      
        1 tttaaattta ttttagatat aaacatcatt ctgactacta ttaaaagaca tataaaataa
       61 ttaaacttaa aagtatattt gcttatcttt ttagtaaata aaatgaaaaa aagaattagt
      121 agacttatgt aaatattaaa tttaataact tctataaata attccataaa atacgtctcc
      181 tattttcttt tttttagcca ttgttcaagt tcttttatgt catcatctga tatttcattt
      241 ccacaaccta atgatgatat aaagctatga atagaatttt tatgtataag ctctaaaaaa
      301 tcctttgttt caaattttag atattcctca tgtttaactt taatagtgta taaagtattt
      361 ctaccatctt tttcaaattc taaaaaacct ttatcactta atctagaaag tactttacca
      421 gtagtacctt ttgtccatcc atgagaaatt tccatataat tagctacttc tctggctggt
      481 attttatcta tgcctttatc ccatatgaaa ttcataacta acaactcgga ttttggtatt
      541 tttttcaata taatcactca cctttaactt gattatatta gacaaatgtc taatagtcaa
      601 tataatatat catcatgtat aaagaatctt tatattattt tatgcttgag aactaagtag
      661 tatagattta tagataaaaa agatattgag agttttatgg aagaggggaa tatattttct
      721 attagagata agaatatgac atatattttt tattgatttg tacaaaaata ctatatagtg
      781 aataaattaa tatcttttct aaattagaag aggataatct tattagataa agattatcct
      841 ctttattgaa agtttaaggg atattagaga ttattaattt ttctctcttt ttttaactaa
      901 aagcaataat acataaaatt attattgcta atgatactct taatgtggta tatagtcata
      961 gtgtcacatg tttatcaata gttttttcat taattattgt aacgaatagt aggactcata
     1021 aaatacttag aaatatgttt ttagatagat tttaaatata taatctaact tatatctata
     1081 aattaacact attattctcc aattccattt ctagtttttt tataaattta ttaaattctt
     1141 ctggttgcct ttttccaaac aatactctta caagagctga attgattttt ccaaatatat
     1201 tcctcttgct ctcactttca ataaaaataa gttttgtatt ttctaagtca atatgtacta
     1261 attcatatct agtaatagta ctttctcttt tattgcttgt ttttatctca tatactctat
     1321 ttttttcgaa attagttatt tcaaccttaa atcttttatt atttttttgt tgtacaaatg
     1381 ttcctactgc ttttttctca ttaaaattag gaaaatttag tcttgcagat tttgtaaata
     1441 tattaaatat gtcttttgca gaatatttta ataaaacttc agttttaaat gtattcatat
     1501 cgttttccct tctgtatata aatttaatat ttataagtta atttttaagt taaaatagta
     1561 taatatgcca tattaaatca tgttaaaact atttatatag ctaaaacctt acctatataa
     1621 gtatgtatac tatcatataa aaataaaatc ttaaaataga aagggttaaa aacacagttt
     1681 attataattt ttattgttga gtagatacgt actattatat atgattgagc aagtttttca
     1741 atcataaatt tataaagtat tttgcattaa aatttaaaca aagagttaaa ttatttaaga
     1801 ttatgaataa ctaatacatt tagtaaatta ctaaatatac aaaatgtaaa tacttatcaa
     1861 aatgtataaa tacttatgac atctagttac ttacagggtt taagtagaaa aaaatatcaa
     1921 agtaagtatg gcatgatagt tgcttatata tttatcaaga aaggagttat taagcatgga
     1981 gttgagagat aaacttgtaa acatcataaa tacagaaaat aaaaaagatc cattaacaga
     2041 tgttcaaata gcaaagtttt taagtacaac tagagagaac ataactaatt tgagaaaaga
     2101 acttaatata agtaattcaa ggcaaagaag atatccttat ttaaaatcag caatttcggc
     2161 aatattacaa aagaataaga atatttctat atcagaaatt acaagggaat tgatgacaga
     2221 aggctttaat atatcgagaa gagttgtgga ggaactttta cctaaagaaa gtttagaaat
     2281 ggatgtagta gaggaatctg aagaagaatc gaatgaccca tttgaaactc tgataggtaa
     2341 taaagggagt cttagaaatg ctgtggagca ggctaaatct gctatattgt atccaccaaa
     2401 aggattacca acgttgatta ttggggaaag tggagttggt aagagtttat tttctagaca
     2461 tatgtatgag tttgcaaagc aaaaaaagat agtaaaagaa agctccaatt ttgttgtatt
     2521 taactgtgct gattatagtg acaatccaca attattatta tctttgttat ttggatataa
     2581 aaaaggtgca tttacaggtg ctgaatatga tacaccaggt cttgtagaag aagctgatga
     2641 aggagtttta tttttggatg aaatacatag attgcctcca aaaggtcaag aaatactatt
     2701 ttctatctta gataggggaa agtttaggag attaggtgaa actaatgctg aaagaaaagt
     2761 aagtattatg ttgattgggg caactactga aaatgtagag actaatttat tattaacatt
     2821 cagaagaagg ataccaatgc taataacttt gccaccatta catgatagat tactaaaaga
     2881 gaaaatagac cttatataca atatctttca acaagagtgt aatagaataa atgcaaaaat
     2941 atttgtagat aaaaatgtga ttgaaatact tgtactaaaa aagtttagtg gaaatatagg
     3001 acaattacaa aatatgattc aggttctttg tgcaagagct tttatgaagt ttataaattc
     3061 caaagatgaa gatagtgaat cgatagtagt agtagatata aatgaagttt taaagcttaa
     3121 agattctttt aaagatgctg cctttcaaga gattgaatat acagagataa gaaaatattt
     3181 gaaaaatgct atatttatac catttaattt agaagaatca tcagtaaaag gaagtctttt
     3241 aagtgatgaa tatatattac cagaggacat atacaaaaca attgaaaaga aatattatga
     3301 cctaaaatcc ctagacattg aagatataga tatagagaat attttatgga cttttatttt
     3361 aaatagattt gctgacttaa agtttaatct cgatagtaat gaagaaattt tttcaatgag
     3421 tgatttaaat agttttgtaa gtgatagttt agttaaactg gtaaaagaac ttatgaatga
     3481 tatagttgaa aaaaatatga atttagaagt aaataaaaat atgttcaaat atctagcaat
     3541 acacttagaa gaagctataa aaagaattaa gctaaatcaa aagataataa atgtaaattt
     3601 agaaaaaata aagattgact tttcaaaaga atatgaaatt agtaaaagtt ttgcaataaa
     3661 aattgaggaa tcaattagta ttaagattcc agatgatgaa attggattta taacactcta
     3721 cataaagtct gctctaaaaa atgaagtgaa aaaagataaa gtagggttaa taataatttc
     3781 tcatggacgt atagctacag agactgtaaa tgtagttaaa gaacttttag gtgtaaagtt
     3841 tcctgtggct atagatatgc ctttagatga aaaaccaata aatatataca ataaggcagt
     3901 agaactgtca aaaataatag accaagggaa aggtatattg tttttggtgg atattggttc
     3961 acttacaaac ataggtcaaa tagtaaataa gagaaccaat ataagcacta aaactattga
     4021 tagagtagac ctcctaatgg cactagaagc aactagaaaa gtttctatgg gagaggaaga
     4081 acttgatgaa atattttttt ctttgctaaa agataggatg ggatataact atgaattaga
     4141 aaaacacata gataaatcaa atgcaattgt aacattatgt cttactggag aaggaactgc
     4201 taagtatata agtaaaacgt tggaagaaaa gtatgataac actaaatgtt atcaaatgag
     4261 tgcattggat gaaaatttat tttgtaaaat tgaaaatcta aaagagacaa acaatatatt
     4321 agctattgtt gggacaataa accctaagat accaggtata aattttatac cctataataa
     4381 agaacttttc aaaaatttgg atatctatct atcaaaatca aaacaacaag atgacagacc
     4441 agttaaatcg tatgaaagaa tgcttgatga ggatttagtg atttttgaac cagatatata
     4501 ttttaaaaag gatttattag aatatgtatg ttcaattctt ataaataaag aatatgtaga
     4561 aaaagattac ttagattctg ttttacatag agaagaaatg cttccaacat attctaaagg
     4621 agctataggt gtgcctcatg gagattcttc tactgtaaat agtacaaggt ttgtttttgt
     4681 taaactaaag aatcctattg attggggtgt aggtaatgta aatttcatat ttatgccagt
     4741 ttttcaggct aatgataaag agattgttaa aaatgttctt gagatactaa aagatgcaga
     4801 atttatgaac aatgccaata agtgttttga taaagatgct tttagaagga ttatatttga
     4861 taaatttaaa catctgtagc catagcaaaa aaattgagag gagttagagt aatgaatgta
     4921 gatttaatat cattgggttt agaatctaat gattatgaaa gtgttattga agaattagga
     4981 agtataatgt gtaaaaaaga atatgtaaaa gaaacataca taaatgctgt cttggaaaga
     5041 gaaaggacat taccaacagg tctagatatt ggagaaatgt gtgttgctat tccacataca
     5101 gattcaaagc atgtaaatga gtctaatgta gctgtaggga tattgaaaaa tcctgtaaag
     5161 tttaattcaa tgatagaccc aaaagataga cttgatgtag aacttgtatt tttattagca
     5221 gtaaagaacc cagattcaca agtaaaactg ttaaaagatt taatgtcagt ttttcaaaat
     5281 ataaagttat taaaaaatat aaaaaacgca tcaacaaaag aagaagtagc taaattattg
     5341 gattttatag aaatttaatg taggagaatt ttataaataa aaaattaaaa tttcaacaat
     5401 caaagtaagt attatttttt taataggttt aatcttatat aaaataaaaa tttagagtga
     5461 aaaattagga ggaaattatt atgaaacaat ttaatgtatt atcagtttgt ggttcaggga
     5521 cagttacatc atctatggta gcaggtaaat taaaagaagt actaggtaat aaggggattt
     5581 ctattactac aacagaagct agaccaacag aagcactaaa tttagcacaa tcaggaagat
     5641 ttgactttat aacatttacg agtccgcttc aaacaggcga ttatggaata ccaactataa
     5701 atgcatttgc ttgcctaact ggtatagggg aagaggcatt ctttgaagaa gttttgactg
     5761 ttattgaagg catagataag taagttttgg agacgtgaat ttatagtatt taaatcaatg
     5821 atttagatac tataataagc taatattaac taaatatttg tggaaaaaac attttatatg
     5881 ttgtcataaa ttaaaaaatt ataaaaatag tcacttaagt attaaattaa gcttataaat
     5941 atttataagt aggagggaaa aagatgttag aatttatgaa atctataatc gatacatttg
     6001 gttctgcaat tatagttcca attataatat tcataatagc aaagatattt aaagtaacta
     6061 ctaagaaatc gtttctttct gctgtgtatg caggagttgc tttacaaggt tttacactga
     6121 ttttaaattc atttacacca ataataacac cagttatcaa taggatggtt gagagtacag
     6181 gagtaaacct accagttttt gatgttggtt ggcaagcaac atcattggtt gcattttcaa
     6241 caagtgcagg tatgatatat ttaggtcttg gtatattatt acaaacaatt ttatttttga
     6301 taaaatggac agacgtgttt caaccatcag atttatggaa taactattct tatatggtat
     6361 ggggagcaat ggttatagga gtaacaggaa attttccttt aggtatagca tgtatggttt
     6421 tattaaactt atatagttta ttaatatcag agttagttgc aaagagatgg tctagttact
     6481 atagatatcc taattgtaca ataatagcta tgcataatgt tgaggcttct gtgtttgcag
     6541 tattttcaga ccctatatac aataaattgg gattaaataa aataaaatta aatccaaaag
     6601 aattagagaa gaaattaggg tttttaggtg aaccaattac tttaggtctt tttttaggta
     6661 tgtttatagg tatattaggt aatatgacga gaataaatac tatggaagct tggggagaaa
     6721 taatgaaagt aggtatatct acaagtgcag tgatggcgat tttccctaaa gtagcaagta
     6781 tgtttgctca agcatttgca cctataacag aagcagctag aaagattatg caaaaagctg
     6841 gaaaccgtga gtggtacata gcagtaaatg atgctgttgg atatggtgaa cctgcaactt
     6901 taatatcagg tcttatatta attccaataa tgcttgtaat atctatggta cttcctggta
     6961 ataaggtatt accagttgtg gatttgttgg caattccata tatggttcaa ggtttagttg
     7021 ccatacataa tggtaatata ccaaaagtat tagtttcagg tataatttgg tttggattag
     7081 gattatatgt ttgtacatct acagcaccat tatttacaga tatggctaca aatataggtg
     7141 tagctatacc tgcaggagca atgttaataa caagctttaa tatactagga aaacctttga
     7201 tgggattagt tttctttgca tttctaagtg caaatccaat ttatataggt ctttcagttg
     7261 taatttactt tgttctatgg gcattgttta gaaagaacaa aacaagtatt cttgattatt
     7321 tagaaaaaca agcacttaaa aatgtagaag aagagcctgt agcagtttaa gtttataaaa
     7381 atgaaataag aataattaaa ggagtttata attgatttta tataagtatc agataatacc
     7441 tgagaaatat ttaaatgaaa tatagacttc tttttatttg ataatatttg taattataat
     7501 gcattgtatt tttatagttg agttttctgt ataacctgct atttaaaaca tactctttta
     7561 tatttataca gtaatgtggt ttaattttat aaaatgtaat ctctactggt aaaagacttt
     7621 gtatataagg catttgcctg aataaaccat ttatcttttg tatggcatag atattgctat
     7681 atagaagtat atagcatgaa aggagatatt tatatgggag ataaaatgag agcatcagta
     7741 ttgtataatg taggtgatgt caggtatgaa atggtagata taccagaaat aacagatacg
     7801 caagtgcttg taaatgtaaa atatgttggg atatgtggtt cagatttgcc taggtctatg
     7861 gtaagtggtt tgagtggaaa tacaaaatat ccattgattt taggacatga attttctggt
     7921 gaagttgtta aaattggaga aaaagtaaag catattaatg taggagatag agtagctgtt
     7981 gcaccattag taccttgtgg aaaatgtgat tattgtaatg aaggtaattt tggcttgtgt
     8041 gatgattaca atataattgg aacaagagta aatggagcgt ttgcagaata tgtaagagtt
     8101 ccagaagaac atattttaaa actaccagat acattggatt atgaaactgc tgccggaatt
     8161 gaaccagcaa ctatagctta tcatgggatt tcaaagtcta atattagagt aggagatagt
     8221 gtagttgtgt taggatgtgg tccaataggt cagtttgtta tacaatgggc aaaggtgttt
     8281 ggtgcatcta agattatagc agttgatatt tttgatgaaa aattagaact ttctaaactt
     8341 ctaggagcaa actatatact taactcaaaa gaagtaaatg taatcaaaga aataaaaaaa
     8401 ataacaaatg gtggtgctga tgttgtaata gagactgctg gtagccgatt tacacaagaa
     8461 caatctctat ttgtagcaaa aaagagagga aatatagtgt ttgtaggtat atcacacaca
     8521 gaattaccac ttagtgcaga cgctactgag tgtatattac gtggagaact taccttgaaa
     8581 ggttcttgga attcatatgc aagcccttat cctggaagag catggactgc aacattggat
     8641 tttatgggaa aaggtgacat tatttttaaa cccatgattt cacataaaat aggtcttaat
     8701 gaagtgggtg actttttaag taaaatgagc aagagagaaa ttaatttcaa taaaatccta
     8761 gtagaaatat aaaaatttaa taaaaaattc taaaaacaat taaattttat aactatgaca
     8821 ttaaataaaa tataggagga agaaaagtgc tagaaatttt aaaagaagaa gtattaaaag
     8881 caaacttaga attacctaag aaaaagctaa ttacttatac ttggggaaat gtaagtggga
     8941 tagatagaga aaaaggatta gtggtgataa aaccaagtgg tgtagagtat agtaatatga
     9001 ctgtagaaga tatggttgta gttgatttag agggaaatgt agttgatggc aaactaagac
     9061 cctcctcaga tactccgact catctagttt tatataaaga gtttgaagaa ttggggggaa
     9121 tagtacatac acattcaagt tgggcaacaa tttgggctca aataggaaaa tctataccat
     9181 cttgtggtac tactcatgca gattattttt atggaagcat accatgtacc agaaaaatga
     9241 ctagagaaga aatttgcaat gaatacgaaa aggaaactgg aaatgtaatc attgaagaat
     9301 ttatgggaag aaatccaatt cattgtccag gaataattgt aaatgaccac ggacctttta
     9361 catggggaaa agatgcaaat gaggctgtac acaatgcagt agttttagaa gaagtcgcaa
     9421 agatggctta ttatacagag ttaatgagtc ctgataatat tatggataag gttcttatga
     9481 ataagcattt ttcaagaaaa catggaaaaa atgcatacta tggtcaaaaa tgattatctg
     9541 gaggtgaatt atgctagact ctttaaaagg taaagttgtg gaaatagcaa ttaaagcaga
     9601 gcattatggt ttatgtaaag aaaagactgg taattttagt attagagata aggatactgg
     9661 atatatactt ataaccccat caggtatagg aagagaagaa ttaaacgttg aacatatttg
     9721 tgtattggat ttgaatggaa atgtaataga agttgagaaa ggtattaagc caagtagtga
     9781 ggtattgatg catattgaaa tatataaaat aagggaaaat ataaatgcta tatcacatac
     9841 tcattcttta tatgcaacag cattttcagt agcaaagaaa ccaattatac caattgtata
     9901 tgaatcagtt aattatggcg gttatgtaga tattgctcca tacgaaagac caggaacttt
     9961 aaaattagct aagagcgttt caaaattact gatagataca gatgcatgct tattagaaag
    10021 acatgggttg acaactgtag gtaatgattt agacgaagca ttattaaaat caagatatgt
    10081 tgaagaggta gcagaaatat actataggtc tttagtatta aatcaattta aagaacttga
    10141 agtagtagat gttgatgagt ttgaatcttg gaaataccca gaagaaataa agttttaatc
    10201 taaataattt tcattgaagt ggttaattaa aatatatgta ttatttatat caattactat
    10261 gttttattat attaatcacc gtattttgta aatatattta ttacattttt tcaaaaaaat
    10321 atagaatccg aaagccatat aaaattgact tgggattcta tactttttat ttatgcattt
    10381 ttctttatct gattttctgg agaatttata tgttcaaaat attttttagt ttcagatact
    10441 attataggtg ataatgcaag taaagctata aggttaggta ttgccataag cccatttaca
    10501 atatcagcaa ttatccaaac aacttctaac tttaagaaag cacctaataa caccatagcc
    10561 acaaatacag ttctataccc attcatgcct tttacaccaa acagaaattc aaaacatctt
    10621 tctccataat aactccatcc caaaattgta gtaaatgcaa ataatgttaa actaactgta
    10681 agtaatattg gtccaaatgt aggaattgct ccgttaaaag ctgcttgtgt cataacagct
    10741 ccatttaggt ctgaacacca cactcctgaa ataacaagag agaatccagt aagagtacag
    10801 attataatag tatctataaa agtacctgtc atagatataa gaccttgttc cgctggccat
    10861 tcagttttag cagcagctgc agctattgga gcactaccta gaccagactc atttgaaaat
    10921 acaccacgag caataccatt tctaattgcc agtgacatag ttgctcctaa aaaaccacca
    10981 gtagcagcag taggtgtaaa tgcactttta attattaaca taaaagtttc agggatttta
    11041 ttccaaaaag atattaaaat gattccacag attacaacat atataactgc cataaaaggt
    11101 actattttag tagctacatt agatatactt ctaagtcctc caaatacaac caaagctaca
    11161 aatactgcta atacaacccc tgtaattcta gggtctatcc cgaaactatt atttattgca
    11221 tctgttattg aatttacttg agtaaaagta ccgattccta aaagtgcaac tagtatacca
    11281 cttatagcga aaaatattgc aagaggtctc catttttctc ccataccatt tactatgtaa
    11341 tacattggcc caccagacac ttgaccattt ttatctttag ttctgtattt tattgcaaga
    11401 acaccttctg aatacttagt agccattcca aagaatgctg ctatccacat ccaaaaaagt
    11461 gcaccaggtc caccagcttt gatagctgta gcaacaccaa caatattacc agtaccaact
    11521 gtagcagcta gagcagtaca taaggctgca aaacttgaaa catcaccact acctttgttt
    11581 tcagcagaga atattaactt aagagctctt ggcagtttag ttatttgaac aactccaagc
    11641 ttaacagtaa gtagtatacc agtaccaact aatagtacca gtaggggtgg tccccaaata
    11701 aaagcatcaa ctttgtttaa aatatcaatt agattcataa aaacactccc tcattttttt
    11761 tgctttacat aatcaataga atttttaatg aagtaattga caggaaataa gtataattta
    11821 ttaataattt ctagtgtaat aaaaaaagct aaatctaaaa aagattcagc tctggatgta
    11881 cactaattag caaccaaaat aaaagtatta gtaaatactg tttttggtag ataatccctg
    11941 tccttttgcc tgagagattg agcataaaag ccttgcccct tcggcgccca taatgggtct
    12001 ctccagagtt ccgtaaattt tacagtccta cttttaaaag tacctgagag tgttactcct
    12061 tcggtgtgct atatagcaac tcttctataa aatcttaacc ggatattgat ttgtcatagc
    12121 ttattgtatt aataatatta atataagttt ttatgttttg tcaactaaaa aattaaaaaa
    12181 caaagaaaaa atataaaaat gtaagcctat ttacgaatta taaacttaga aagatgagta
    12241 atatacgtaa aaatgaattt tgtaatacac atacaaatga agtgtaaaat aagttttaaa
    12301 taaaaaaata ataaaatctt aaataattaa ttagatatga ttaaattatg gtaatattat
    12361 agttacgata ttaagaaaag ttctatttag ttagatttta agcgtaatat gagattatgt
    12421 atagagaaag gatgtatttt tagatgggaa aaatattagt gctagcagaa aaaccttctg
    12481 ttggaagaga tatagcaaaa gttttagggt caaaaaatga aaaaaatggt tatatagagg
    12541 ggccaaagta tgtgataact tgggcactag gtcatttggt tacacttgct gaccccgaaa
    12601 gttatggaga aagatataaa tcatggagct tagaagacct gccaatactt cccaagcacc
    12661 ttaagactgt tgtaataaaa aagagtggaa agcagtttaa tacagttaaa tcgcaaatga
    12721 atagaaatga tatagatgaa atagttatag caacagatgc aggtcgtgaa ggagaacttg
    12781 tagcaaggtg gataatagaa aaatcacagg taaaaaaacc tataaaacgt ctttggattt
    12841 cttcaagtac agacaaagca ataaaagacg gatttgcaaa attaagaagt ggaaaagaat
    12901 atgaaaattt gtattactca gcaatagcac gagcagaagc tgattggatt attggaataa
    12961 atgcaaccag agctttgaca acaaagtaca atgcacagct ttcttgtgga agagtacaaa
    13021 ctcctactct tgcaatgttg cttaaaagag aagaagaaat aagaaatttc aaacctaaag
    13081 agtattatgg attagaatta attgctacaa agggaaattc agatataaaa tttatttgga
    13141 atgataaaaa caataattct tcaactttta gtaaagaaaa gatagaatcc atattaaaaa
    13201 aagtaaaagg tgtagacata aaaatatctg aagtaaacaa gtcatataag aaaaaatatt
    13261 cacctgcact ctatgattta acagaattac aaagagatgc aaataaaatg tttggatttt
    13321 cagcaaaaga aactttatct ataatgcaaa aactgtatga acatcataaa gtacttactt
    13381 atcctagaac tgattctaga tatttaacat cagatattgt tggaacatta aaagatagaa
    13441 taaaagctgt aaatattagt gattattcaa agatttgtaa taaattatta aaaagcaata
    13501 ttatagctaa taaatcgttt gttgacaatt caaaggtaac agaccaccat gcaataatac
    13561 caacagaaca gaaggtattc atgagtgatt ttagtgacaa agagcgaaaa atatataatc
    13621 ttgtagtaaa aagattttta gcagtactat ctcctccttt tgaatatgag cagacaacta
    13681 taagtgcaac tgtagaagga gaaatattta aggctaaggg aaacaaagta ataaatttag
    13741 gttggaaaga aagttataaa gatattgaag atgaagatga gtatgaaagt ataccaaatg
    13801 ttttagaaaa agatgtactt aaggtcaagg gattaaagat aacttcagga aaaacaaatc
    13861 caccaccata cttaaatgag ggaacattat tgactgctat ggaaaagaat ggtctaggga
    13921 cagttgctac aagagcagat ataatagaaa aattattcaa ttcattttta atggaaaaga
    13981 aaaataaaga gatatatata acatcaaaag gaagacaatt attagattta gttccagtag
    14041 acttaaagac accagaactt acagcttcat gggaaaagaa attattggat atttcaaagg
    14101 gaaaacttag taaaaataca ttcataaatg acatgaaaga ttattcaaaa gatattgtac
    14161 tagaagtaaa aaatagtgat aataaattta aacatgataa cttaactaaa aatagatgtc
    14221 cagaatgtgg taaatttatg ttagaggtaa atggtaaaaa aggtaagatg ttgatttgtg
    14281 aagatagaga gtgtaatact agaaaaacta tatcacaagt aaccaattca agatgtccta
    14341 actgtcataa aaaattagaa cttcgtggag aaggtgaggg gagaatcttc acttgtagtt
    14401 gcggatacag agaaaagttg tcttctttca acaagagaaa gcaagaagaa aaacagaagg
    14461 catcaaaaaa agatgttaat caatatttaa aaaatcaaaa taaaaatcaa gagacattta
    14521 ataatccatt tgcagaagca ttagctaagc taaaaaacaa ataattttaa agttattatt
    14581 atacacaatt ataactttaa ctgtatatta gttatatgta ctgatataat atagtgtata
    14641 gtttaggcat gaaagtaggg ctgttaaatg gatttaagtg gcatatttaa atactattgt
    14701 aaagagtgtg aaaatacatg gaataattcg agtgttgaat tatttgagga tatagaaacg
    14761 tatagtaaag attcacaaaa aaagagggaa aaagaattag ataaatttat aaatacaata
    14821 tcagttcatt tagagaggta tccaagtgat gctgtattga gaaaaatgtg ggtaaaaaag
    14881 ggcgaggttt tcttacaaaa gacattggaa aaagaaaata tttttaagtt agaaaaaatg
    14941 gatgtagagg atagaaaaaa atttttagat ataacaaaac agtttattag agatgctaga
    15001 aaatttgatg atgatttacc tataggtgat attatgcaag ctatgaggaa tgtatggatt
    15061 tcaaatgcat tacaattatt atttggtaaa gaaatatatt attcaaaagc taactttgca
    15121 tatagtatgt tatatccata tactgataat tatttagaca atacaaatat agataaaaat
    15181 gataagattt tatttaataa ctggttagaa aaaaggctcc tgggagaaca tattaaatct
    15241 aaggattatc atgaaagtaa agtatctcaa atgatagatt atattgaaag tgtataccct
    15301 agagaaaagt ttacacaagt ttatgaatcg ttattattaa tatttaaaag tcaagtaaat
    15361 agtttaaaac aacatggtaa ggaaaatcat ttgtgtaaag aagatttatt atccatttct
    15421 attgaaaaag gaggttcatc cgttttagta gatggatatt taataagtgg atttatgaca
    15481 aaggaagaaa tagagttttg tataggatat ggatttttat tacaaatatc tgatgattta
    15541 caagatataa aagaagattt aaaatacaac cataaaacta ttattacaga gatgtcaaaa
    15601 gagggtactt tagataaagt tgtaaataaa ctaataaatt ttactattga gttaatagat
    15661 agttttaaaa ttaataataa aaataaatct gtaataacta tgataaagaa tgattgctta
    15721 atgttaattt tattttctgt agtttataat gctgaatttt tttctgtagg atatataaaa
    15781 gaagtagaga aatttattcc atatacaata gattattcat tagagattga agaaaaaata
    15841 aaagaaaaat ttaaaaatat agatgtttta aataatgaaa atgaatataa agaaatgatt
    15901 gatattattt gtgcagagta gaaaaaaagg gagtgtctta gaatagactt tttagtttat
    15961 gaggcactct ttttgtataa aatcaaatgt attttcatca ttttaacaat taaaaagagc
    16021 ttatccctgt ttgagaaagc cctttttaat ttaaataaaa atgtcaaggt taaactattt
    16081 aaatgcagta ataatcccat tatcatgtaa gttttttatg attgtagctg cgatagggaa
    16141 tccaaataat ccagctattc caaatagttg agctccaata aacatacaca ttaatgttat
    16201 taaaggatat aggccaattt ggtgtcctac tacctttggt tctaatattt gtctaacaac
    16261 ggttataatt atgtatagta ttaaaagtcc tattgctaaa ttaatatttc cattaatgag
    16321 tgagataatc atccaaggtg tcaaaatact tcctgtaccc aagactggca aaatatcaac
    16381 aagtgctatg atgacagata tagtaaatgc attttcaacc tttaggatag taagtccaat
    16441 aagtagttct aagaatgtca ctgatagtag tattgcatag gcttttagta gttttaaaag
    16501 agcattaata ctgctatttt ttatatttat tatcataaat tgactttttt ctggaaattg
    16561 ttttaagata aagtttacta ttttttgata gtcaaaagta aagaaaaaag aagcaattaa
    16621 agtaataata agttttaaca taaatgcagg tgttttactt gcaatactag caatagcatt
    16681 gagtgcattt gttgaaatag aagctactaa agacatcata gattgactta tattatccca
    16741 accaagtatt aatgataggt taacattagg tgtactagca tctatctttg aaaataaagt
    16801 atttagcgtt ggttgtatac ttgatttata tatttcagga agtctaaaaa atacatcact
    16861 tacacttgca aatatcttca caccaaagct aactaataaa aatatacaca acccataaaa
    16921 aattaaaagt attactatag aaataagtgt acgatttaaa tttgtattat ttgtaataag
    16981 tcttataata ggtcttaaga tggatgcaat gataaaactg attacaaaag gcattaataa
    17041 tggcaataag aattttaata caatatatac aatacttgtt attaaaacta catatgtaaa
    17101 tttaattata aaattctttt gcttttgaat atccataata taaaacctcc aatgtatttg
    17161 atattattct aaatatggtc ttttttacag aaaatatact actgtatatt gattataaac
    17221 tataaagctt atgtaaagta aataaattat gtaaaattta ggttaatttt gtataaagag
    17281 atacttttat attttacaat taataaccaa atactaaaaa atattttaaa taaagaactt
    17341 aaaattgtga taaaattaat ttatagatat taaaaaaagt ataatatatg gtttttaaga
    17401 ggtgctaaga tgaaaaattt tagagattta ggtgggaaca aaactgagga tggcagaact
    17461 gtaaagaagg gattatttta taggtctgca aaattatcaa atctaagtga aaatgatata
    17521 aagattttaa aagagttaaa tattaaatat atatttgatt atagaagtga tgaagaagct
    17581 cgtaaacatc catcaacaat aatatcaaat ataaaaaata taagaatacc agcaatgaga
    17641 gagctagaag aatcaggagg aagttttggt tctattgaag atatgattga tggattgttt
    17701 gaaaaagatg gtgcatttaa tatgttaaat aatagttact ataatttgcc tataaataat
    17761 ccgtcatata aaaaattagt agaacttata agagactatt ctaatttacc aatattaaat
    17821 cattgtacag ctggtaagga tagaactggt gtggggagtg ctataattct tatgatttta
    17881 ggggtatcga gagagaatat aatgaaagat tatctaaaaa gtaatgattt tgcagataaa
    17941 gagatagaaa gatttataga atataagcct aaatttaagg acattccaaa agaaaattta
    18001 aaatatatat ttggtgtaaa tgaagagtat atgaaaactg catttagaag aattgatgaa
    18061 gaatacatta gtgtagaagc atatttatat ggagaattta atttaaataa agaagaaata
    18121 agaaaattaa gaaatcaata tctagagtaa aaggtgagtt tatgagttta aatataacag
    18181 caagacagat aaatataata aaaatattgt taaattcaaa agatacaatt agtggtattg
    18241 ccttaagtca agagatagga tgttcttcaa aaactataca aaatgaaata aaggatatta
    18301 ataaacaact tagaaatggt aaaattttgt caataagagg agttggttat aaactagaag
    18361 gaagttttga tgaggtaaat ctaaatagca atctctatga tgatgtagat agagtagaat
    18421 atataatcag aaaaatatta accttaagta atgaagaaaa aaatactata aaacttgaag
    18481 atttagccga ttctatgtat gtaagtgttt caacagtaaa aaatgattta aaagaagtaa
    18541 aaagaatatt ggaaaagtat tcaatatcag ttaaatctaa gcataaacaa ggtatatgtg
    18601 ttttggaaag tgaagataag atattaaatt gtatagttga cttaagcaat aagagagata
    18661 atcaattgag tttaaatgat tttttaaatg acgatataaa ggaaagaaaa ctattaataa
    18721 aaaaattatt attagacata ttaaatgaaa atggattagt attaactgat atagaattta
    18781 agagtgtgtt aaataatata ttagttatgt taagcagaca taagtataat gaaaaagaat
    18841 ttatagagaa ttatatagaa aattatagga gcaaaagaag ctcaatttta gaaaatgaac
    18901 aaaacaaaga aattataata aattcaataa aaacattttg taaaaattta aaattagcta
    18961 catcaataga tataagtaat gataaggtat ttgaagagtt tttatataag catattagta
    19021 gtttatgtaa aaaaataaag ttaggtataa atcaatcacc tataatggct caagatataa
    19081 aaataaaata tccatttgct tttgaactag ctaatatagc taaaaagaca atagaaaaag
    19141 atttgaagat agaaattaat gaagatgaag ttgcaaacat agctctacat ataggaggag
    19201 cagttgaaag agcatcttat aataataaag ataaggtttt caaaactata atagtttgta
    19261 cttcaggcat aggcacatca atgttaataa aagctaaatt agagaatata tttaaggaaa
    19321 aattagaaat actaaagatt attccttcat acttagttga atatataaaa gttatagatg
    19381 ttgattttgt aatatctact gttcctattg aagtaggaga tgtgccagta ataaatatat
    19441 ctccaatttt aagtgagaaa gaaattagat taattgaaaa atatatagaa acaggaaata
    19501 tatacctaga tttagatata aaaagcctgt ttgatagtga attatttttt actgatttgg
    19561 tgtttaaaag gaaagaagaa gttatagatt ttatggcaag taaacttgta caaaagggct
    19621 atatagatga ggatatgaaa aaatcttact ttgagagaga aaaaatagca acaacagaaa
    19681 tagggaatat ggtaactata ccacatggag ctattggaaa aatttttgat aataaaatag
    19741 caattggtat attaaagaag cctatttctt gggaaatatc agatgttcgt ttaataataa
    19801 tgttagccct tgataaagat aaaatattag attatgaagt aatattttca aaaatatata
    19861 aaagagtaga ttctatagca aaagttataa gtatatgtga aaatagtagt tttgaaaaat
    19921 ttgtaaattt atttaattag atgggtagta ttgtatatac acctgtaaat ttggataata
    19981 taaaactaaa taagataaaa acgataaatt tctcttttaa ttaagagaaa ttttttgact
    20041 ttcaaggata ttaatataaa gttaagatat aaacataaag caaataaata aaaaagaggt
    20101 ggcaatatga tttatacagt gacattaaat ccatcaatag attatatagt taagctagat
    20161 gagctaaaga caggaagtac aaatcgtgta aaagaagaat acgtttatcc aggaggaaaa
    20221 ggtataaatg tatctcgcat acttaaggaa cttggaaatg ataatacatc tttaggtttt
    20281 ataagtggtt ttactggaga atatataata agaacattgg aagaaaaaga attgaagaca
    20341 gattttataa agataaaaag tggtttttca agaataaatg taaaaataaa ggcgttaaag
    20401 gaaactgaga ttaatggaca aggtccaaat atagatgatg aagacattga tactttatat
    20461 aaaaaacttg ataagctaaa tcaagatgat attttaatat tggcaggaag tataccatct
    20521 actttagatg aaaaactata cgaaaatata atggctagat tagaaaaaaa gaatataaaa
    20581 gttgtagtag atgcaactaa aaatcttctt cttaatgttt taaaatacaa accttttcta
    20641 attaagccta ataacgacga attagaagaa ctatttggtg ttaaattaaa ttcaattgaa
    20701 gacatggtaa aatatgctaa aagattaaag gaaatgggag ctataaatgt cttggtatca
    20761 atgggaaaag atggagcatt gttgattaca gaggaagatg aagtgcttat aagtgatgtc
    20821 cctaaaggaa aagtaaaaaa ttctgtagga gcaggtgatt caatggtagc aggatttata
    20881 tctgggtatt tgaatactgg gaaatatgat tatgctctta aattgggagc agcttcaggt
    20941 agtgcaactg ctttttcata tgacttagcc aaaagagaat acattgataa attggtaaat
    21001 gagatatctg taaagcagtt ttaaaatatt aaaaaatata tagaaaggta attaatattt
    21061 atgaaaatta tagatttatt agatgaaaag tcaattaagt taaatttaaa ttcaaaaacc
    21121 aaatcagaag caatagaaga attagtagat ttggtcgcaa atagtggaaa tttaaatgat
    21181 aaagaaaact acaaaaaagc aattttggct cgtgaagaaa tgagtacaac aggaattgga
    21241 gaaggtgtag ctatacctca tgctaaaaat tcttcagtta ctaaagcttg tatagcagca
    21301 gcagtgtcaa aagaaggtat agattatgaa tcgtttgacg gaagtttatc aaatctattt
    21361 tttatgatag ctgcaccaga cggagctaac aatactcatc ttgaagtatt atcaagatta
    21421 tctacaatac ttatggatga agactttaga aataaactta taaattcaag ctctgaaaaa
    21481 gaatttaagg aaataataga taaaaaggaa agagaaaaat tcagtgaaga atatcaagat
    21541 gagaaagtgg ctgaaaaaaa tataatagat actaaagaga atgatgctaa taagtatcct
    21601 aaggttttag cagtaacagc atgtccaact ggaatagcac atacatttat ggcagctgaa
    21661 agtttaaata aaatggcaga aaacaaaggt gtatcaataa aagtagaaac taatggttca
    21721 gcaggtgtta aaaataagct tacaaaagat gaaatagaaa atgcaacatg tataatagtg
    21781 gcagcagata aaaatgttga gatggctaga tttaatggca aaaaagtcat aaaaactaag
    21841 gtggctgatg gtatacacaa agcggaagaa cttatagaca aggctgtaaa tggagatgct
    21901 ccaatttacc atggaggaga tggaagtcac aacgaatcaa atgaagaaag tgaaagtgga
    21961 tttagaaaag tatacaaaca cttaatgaat ggtgtatcta atatgttacc atttgttatt
    22021 ggtggaggga tacttatagc gatagccttt ttacttgatg attatactat taacccttca
    22081 aactttggtt caaatacacc gatagcagcg ttttttaaag ggataggaga taaagctttt
    22141 ggatttatgt tacctgtact tgctggatat atagcatact ctatatctga tagaccagcg
    22201 tttgttgtag gatttgtagg aggtgctcta gcaggtgatg gtggttcagg atttttaggt
    22261 gcactacttg caggttttat agcaggttat ttagtagaag ggcttaagaa aatatttagt
    22321 gtactaccag cttcattgga aggaataaaa ccagtattgt tatacccttt acttggaaca
    22381 cttttgatgg gaattataat gacattttta gtaatacctc cagttacagc aattaataat
    22441 gcaatggttg gatttttaaa tggtttaggc ggaacttcaa aaatagtttt aggattagta
    22501 ttaggtggaa tgatggctgt ggatatgggt ggacctgtaa ataaagcagc atatgtattt
    22561 ggagttgcat cacttgaatc agggcagttt gaaataatgg cagcagtaat ggctggtggt
    22621 atggttccgc cacttgctat agctcttgca actacatttt ttaagaatag atttactaaa
    22681 gaggaaagag attctggtaa agttaattat gtaatgggat tatcatttgt tactgaagga
    22741 gctatacctt ttgcagctgg agacccactt catgttatac cagcttgtgt tggaggttca
    22801 gcagtggcag gagcacttag tatgttgttc aacgcagctc ttcgtgcacc tcatggagga
    22861 gtatttgtta taccagtggt tacccatcca tttgcatata tattagctat agctgtaggt
    22921 gctttggttg gaatgatgct tttagcatta cttaaaaaac cattaaatca agaagtatag
    22981 caattaaagg atattcaaga acttaaaata tgatgtttta taaaaattaa tatattgctg
    23041 aattatttta aaataaggat tttattaaaa taaggatttt attaaaataa ggattttatg
    23101 aggctgttat ataacgattt gtaattgtta tcttgacagt cttttatttt tatataagta
    23161 aaataagtgt gttttgct
//
LOCUS       DAMJVX010000043        22660 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMJVX010000043 DAMJVX010000000
VERSION     DAMJVX010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.045"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(84..923)
                     /locus_tag="PC407_001585"
     CDS             complement(84..923)
                     /locus_tag="PC407_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDF2497037.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            complement(944..1477)
                     /locus_tag="PC407_001586"
     CDS             complement(944..1477)
                     /locus_tag="PC407_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDF2497038.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            complement(1489..2298)
                     /gene="aroE"
                     /locus_tag="PC407_001587"
     CDS             complement(1489..2298)
                     /gene="aroE"
                     /locus_tag="PC407_001587"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2497039.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRIFEILNIESAYKNFEIPKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            complement(2345..3541)
                     /gene="pheA"
                     /locus_tag="PC407_001588"
     CDS             complement(2345..3541)
                     /gene="pheA"
                     /locus_tag="PC407_001588"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDF2497040.1"
                     /translation="MEDLTRYRNEIDNIDKEIIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAIDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKTISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYELEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAENNINMTKIESRPMKNASWRYFFYIDFECSIYNSKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            complement(3544..4623)
                     /gene="aroC"
                     /locus_tag="PC407_001589"
     CDS             complement(3544..4623)
                     /gene="aroC"
                     /locus_tag="PC407_001589"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDF2497041.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDKIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQRDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            complement(4601..5914)
                     /gene="aroA"
                     /locus_tag="PC407_001590"
     CDS             complement(4601..5914)
                     /gene="aroA"
                     /locus_tag="PC407_001590"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDF2497042.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNSTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            complement(5914..6978)
                     /locus_tag="PC407_001591"
     CDS             complement(5914..6978)
                     /locus_tag="PC407_001591"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDF2497043.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            complement(7000..8013)
                     /gene="aroF"
                     /locus_tag="PC407_001592"
     CDS             complement(7000..8013)
                     /gene="aroF"
                     /locus_tag="PC407_001592"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2497044.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKIVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            complement(8143..8298)
                     /locus_tag="PC407_001593"
     CDS             complement(8143..8298)
                     /locus_tag="PC407_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497045.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            complement(8475..9431)
                     /locus_tag="PC407_001594"
     CDS             complement(8475..9431)
                     /locus_tag="PC407_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HDF2497046.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFIGIIIFKIKKLL"
     gene            complement(9770..10468)
                     /locus_tag="PC407_001595"
     CDS             complement(9770..10468)
                     /locus_tag="PC407_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2497047.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            complement(10502..13204)
                     /locus_tag="PC407_001596"
     CDS             complement(10502..13204)
                     /locus_tag="PC407_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDF2497048.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEVITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSSKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            complement(13382..15199)
                     /gene="ftsH"
                     /locus_tag="PC407_001597"
     CDS             complement(13382..15199)
                     /gene="ftsH"
                     /locus_tag="PC407_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2497049.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            complement(15230..15673)
                     /locus_tag="PC407_001598"
     CDS             complement(15230..15673)
                     /locus_tag="PC407_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2497050.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            complement(16164..17054)
                     /gene="metA"
                     /locus_tag="PC407_001599"
     CDS             complement(16164..17054)
                     /gene="metA"
                     /locus_tag="PC407_001599"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDF2497051.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(17084..18349)
                     /locus_tag="PC407_001600"
     CDS             complement(17084..18349)
                     /locus_tag="PC407_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HDF2497052.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(18459..18561)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(18615..21272)
                     /locus_tag="PC407_001601"
     CDS             complement(18615..21272)
                     /locus_tag="PC407_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2497053.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNFSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKRKK"
     gene            complement(21400..22143)
                     /gene="yaaA"
                     /locus_tag="PC407_001602"
     CDS             complement(21400..22143)
                     /gene="yaaA"
                     /locus_tag="PC407_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDF2497054.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIIN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            complement(22153..22617)
                     /gene="bcp"
                     /locus_tag="PC407_001603"
     CDS             complement(22153..22617)
                     /gene="bcp"
                     /locus_tag="PC407_001603"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDF2497055.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
BASE COUNT         7065 a         4068 c         2442 g         9085 t
ORIGIN      
        1 tattgaatcc atatttatta atacaattaa atacactatc gcttacattt atacccatct
       61 atatatagct taaatattaa tatttatttt tctaaagctt ctcttcttat tgatgatttt
      121 ttaaaatact ctatcaattt atttttatca ttgttaaata tagcttcttt tactaaattt
      181 acttctgatt caaagttttc tataactttt agtaaattat ctctatttcc taaaaaaagt
      241 tcactccata agtcttcatt catatttgca attctagtta aatctctata actatctcca
      301 ataaatttac ctgtatctct accttcttca tcactattta taagagcaac tgccataaca
      361 tgaggtaact gagaagtaaa agctataatc tcatcatgtt tttgtgaagt taactttttt
      421 actcttttaa atccaatctc taaaattaaa ttttcaacta actctaaatt ttttatgttg
      481 tttctgccag ttggagtaat tatataattt gcgccattaa aaacttgttc acttgcaaaa
      541 tcaataccct ttttttccct acctgccatt ggatgtccaa atataaaatc tatatcatct
      601 ggaataattt gcaacacatc attgataagt gtttctttta ttcctgtagt atcagtaatt
      661 atagtccctt ttttaaaaaa gtttttatta ttatttagaa aatctacaac taatctagga
      721 tatatagata gtataattaa atcagctttt ttaaataact ctttacctgt agtacatcct
      781 ttttttatga tttttgcttt ttctgctttt tttaaagttt ctaaatctac atctactcca
      841 aatacatttt catatccagc tttttttaat gctttagcaa aagagcctcc tataactcct
      901 agtcctacaa taacaatatt cattattttt atttccccct agattatttt tctttaaagt
      961 caaatttaaa attttcactg actgcatttg taatattata tacagcatta tttaatgatt
     1021 tatcatttaa aatctctata tctgaaaatt ttttataaag atttattctc tcattataaa
     1081 gattatacaa tctatctttt ccgtttttta ataatggtct tgaattaata tttatatctt
     1141 ccaaaatctt ttgtataggt ctatcaataa aaattattat acctgtttct tttagtattt
     1201 ccatattaac atcctttttt attacacctc cacctgtaga tatcactgtt ttatcactta
     1261 tagaaagttc tctacatgct tgagtctcat agttcctaaa gatatcttct ccttctgaaa
     1321 acaactctgc tatacttttt tgagatattt gaattatata atcatccata tcacagaaac
     1381 tacaattata ttctttggcc aaaagtttac caattgtagt ttttccacta cctggcatac
     1441 ctattaatat caatttttct tttgttttat ttatcatgac aatctccctt acaaaaattc
     1501 taattttagt tcattatata taacatctaa aatactatta tctattttag tatcttgcca
     1561 aatttcctga gattttattg cttgccctac cagcatatat aatccatcac aagttttttt
     1621 acccatacta ttaccaattc ttaaaaactc tgtttctcca ggattgtaaa ttaaatctat
     1681 taatatatca aaattttgta taatagattt tgacactggt gaaattccta cattagggta
     1741 catacctact ggtgtagcat ttaatattat atctcctttt atatgtttaa gttcttcata
     1801 atctattaat atagctttac tatttaataa ttttttatca tccttttttc tagttgaaac
     1861 ataaagcttt tctataccag aatcaataaa atatgtaaga gcagctttag aagcacctcc
     1921 agttcctaat ataactgcta cttttccttg aacatcaata ttagccattt taaacatact
     1981 atcaagacca aagtaatctg tattgtaacc atataacata ttttctctga gtaaaattgt
     2041 attaactgct cctatacgtt ttgcctctgg tgatataaaa tcaagatatt tcataatacg
     2101 ctccttatat ggtacagtaa catttactcc ttgtattcct aataatttta tagcaccgtc
     2161 tagcttagat atatcttctt ttggaatttc aaagttttta taagcacttt caatattcaa
     2221 tatctcgaat attctcttgt ggatttgagg tgaaacactg tgagagagtt tttcacctac
     2281 tagaccgaaa aaattcatac ttacacctcc taataattat aaattaaata aaaatttata
     2341 ttatctattc agttttattt ttatatactc ccataaattt aaaatatgct gtattatgtt
     2401 ctatcaattc taataagtcg tacactttag aattgtatat agaacactca aaatctatat
     2461 aaaagaaata tctccatgag gcatttttca taggtcttga ctctattttt gtcatattta
     2521 tattattttc agcaaagtat ccaagtactt tataaagttt tcctgcttca tgttcaacag
     2581 aaaatactac actcattttg tttgattctt cttcaatgtg tatttgatta ctgattacta
     2641 caaatcttgt atagttgttt gcaatatcat ttatacaagg acttattatt tctaattcat
     2701 aaatacttgc agcttttttg cttgcaattg ctgcttttgt tttatcttga agttttctta
     2761 caagttctgc acttgttgct gtgttattaa atggtatcaa tttccatgta ctattttgtt
     2821 ttaaaaattc actacattgt cctatacctt gtggatgaga atatatttct tttatatctt
     2881 caagttttgt gtcttttatc cctattaaat tttggtctat ttttatacat tcttctccaa
     2941 ctatataaaa accgtacttt ctcaaaaggt cataagtttc agagatagct cctgtagatg
     3001 aattttctat ggggattatt ccatagttta tttctttatt tttaagtgct atgaaaacat
     3061 cttcaaattc tttaaattgt ttttttgata ttgttttgtt gaagaattta tttagtgctt
     3121 cttctgtaaa tgaacctgat tttcctggaa atcctactag gatatttttt ttatttaatt
     3181 catcttttaa agacttatat ttatttacat ttgacaaatc aaatttctta tttgatgaat
     3241 ctaaactaaa ctcgattttc tgctcaccat cattatctat caatcttttt tgtaatcctc
     3301 tactaatttc cattatagaa ttaaaaaact gtaccgtttc ttgtgagtat gctttattat
     3361 ctaaattatc tatggctcta cttagtactt tttcttctct atctttttga agtatagtag
     3421 tgttattttt cattttatgt ctggcaattt caagaactgt attcattctc ttttcaaata
     3481 attgaataat ctctttatct atattgtcaa tctcatttct atatctcgtt aaatcctcca
     3541 ttattatcgt cctttcatca aatcaaaaat tccaatggct gttactgctt ctattacagg
     3601 tatagccctt tgaactatac aagggtcatg tctgccttta atatataaaa tatcgtcctt
     3661 tttatctttt atattaactg tgttttgttg tctgctaatt gaaggagttg gcttaattgc
     3721 cactttgaaa attattggca ttccagtagt aatccctcct ataatccctc cattgttatt
     3781 tgttttgctc ttaacttgat ttccttcata atacatttca tcattacttt gagagccata
     3841 catatctgca agttcaaaac ctaaaccaaa ctctacacct tttacggcag gaacagaaaa
     3901 caataaatgt gataaagtag attcaacaga gtcaaaaaat ggatttccaa gccctacatt
     3961 aatacctact actgcacact caattatacc accaactgaa tctctttgat tttttgcatc
     4021 tataatcgta tttttcatgg cttcttcctt tgataaatca agtaaaggta gttccaaagt
     4081 ttgcaaattt gataactgtt gttttgaaat atttacataa tcaaaactca tatcttctat
     4141 atttttaatt ttttttatgt gtgctcctat ctctattcct ttttgtgata aaatttgttt
     4201 acatatagcc ccacaaaata ctaatggagc tgttatccta cctgaaaaat gacctccccc
     4261 tctatagtca ttaaatccag aatatctcac attacctgtg aaatctgcat gtcctggtct
     4321 cattaaattt ttaagctctc cataatcttt tgaacgagta tcactatttc ttatgattgc
     4381 acataatggc gttcctgtag ttcttccatt aaaatatcca cttagaattt ctggtatatc
     4441 actttcattt cttgaagtag atatagaatt ttttccaggt gctcttctct tcatttcttt
     4501 atcaatctta tcaaggtcaa gttcaatccc cgaaggaaga ccatctatat ttatacctat
     4561 agcatttcca tgagattctc caaaaattga tacttttaaa ttatttcccc atattccact
     4621 catcaattcg acctcctaac atcttaaaat ctttaaaaaa atgtggataa gattttgata
     4681 cacactcaaa atcttttaat attattggtt tatcacatct acaagaagca attgcaagtg
     4741 tcatagcaat tctatggtct ttgtgactcc acacctctac tccaccattt aacttgctta
     4801 ctccttcgat tatcaaacta tcctctttct cctctatatt tgctccaagt ttacttaact
     4861 caacatttat tgcatgaagt ctatcacatt ctttaattct aagcctacca gcattaatta
     4921 ttgtagtcct ccctatactt aaggatgcta ctacagataa tacaggtatt atatctgggc
     4981 attgagatgc atctatcaaa gtactattta aaccatttgt tattcctttt attttgttgc
     5041 cttctcttaa tatttccatt cccatcctag ataaaatttc aactacttca ctatcccctt
     5101 gaagagaatc ttctttaagg tctaatatgc taatatcttc tcctatagca tctgctgata
     5161 aataaaatgc tccttgagaa taatctcctt ctactttata atctctcgct ttatacgttt
     5221 gatttccttt tataataaat tctttatagt tattgtttat aatttctacc ccaaaatctt
     5281 ttatcgttga aagagttaaa tctaaatatc ctttagattc tagctcagta gtaattatta
     5341 tctttgaatc actctctagt gtaggcaata taaacaatag acctgtgata aactgagaac
     5401 ttatatttcc ttttactcta aattcatctg gtttaagctt acctgatata atcaaatcta
     5461 actgattttc tttgtaacta tattttatat tctgtctgtc aaatatctca taataagtat
     5521 caagtggtct ttttccaaga ttacctctac ctataaacct ttttacacca tcaaatgcta
     5581 aagatatagg tactaaaaat cttaatgtag agcctgattc attacaatct attgtcaatt
     5641 taggttgatt caactgattc tctctattta aaataccttc tttagaaaaa attcccgaaa
     5701 tttctaaaat atcttctttc ttttctataa tagctcctaa tgatgtcatt gctcttatag
     5761 tagctattat atcatcagaa aaatctatat tagaaattct actttttcca ttgcttagag
     5821 atgaacaaat aactgcccta tgtgccatac tttttgatgg tggtattttt acatctccag
     5881 ataatttact tggatatatt tttagacttc ccattatctt acctcctgaa aaaatttaga
     5941 agtagtatta tatataatac tctctccaat atcttttaaa agtattattt ttaatgtatc
     6001 acctagattt tttttatcaa gagatatagc atctactata gatttattat ctcttaattc
     6061 aatctcataa ggcaaattat aattcattaa aattgatttt ataagttctg atgtaccttt
     6121 ttttgtaatt cctatctttt cactttctaa agttatctta tacattccta tggcaacagc
     6181 ttctccatgg gtataacccg taaaattgta gtatttctca atagcatgac ctaatgtatg
     6241 gccaaagttt agaagcattc tatctccaaa gtctttttca tcagcttcaa ctacagtttt
     6301 tttaataaag caacatttat gtataatgta ttctatatgt tgcattactt cttctctatt
     6361 ttttaaactt aaaagtttat tgaaaaaagt tttgtctttt atacatccat attttataac
     6421 ttctgccatt ccatctctat agaatttttc tggaagtgat aataatacat ttgggtcgat
     6481 aaacacagct tttggttggt aaaagcttcc cactaaattt ttgcctctct caaggtcaac
     6541 tccaactttt ccacctacag aactatcaac ttgtgctaat aaagatgttg gaatttgaat
     6601 aaaacttata cctcttaaaa aagttgctgc tgcaaatcca cctaaatcac ctattacccc
     6661 tcctcctaaa gtaataatca aatcttttct agttaactta aagtcaagta aactatcgta
     6721 tattttagga agagtattaa atgattttgt ttcttcacca gattttaata ttaaagattt
     6781 aatttcaaat ccagattcac ttaaattatt ttttacttta tccaagtata attttgctat
     6841 attttcatca gttacaataa aaatcttatt tccaaaatat atttttttta tttcttctcc
     6901 tatattcttt agtatatttt ttttaataaa tatattgtag ctattttctt ttaaatttac
     6961 taatagtttt tcttccacgt ttaaccatcc ttttatagat tatatttctc taccaactgc
     7021 tgatgcgata gtctttaatt cacttatcaa ttcatcatat tcatttggct taatcgattg
     7081 ttttccatca caaagtgcat gtgctgggtc attatgaact tctattatta gaccatctgc
     7141 tccaactgca attgctgcct tagataaaga gtcaaccatc catgatttac ctgctgcatg
     7201 acttgggtca actataactg gtaaatgact aagtttcttc acagctaaaa ttgcacttaa
     7261 atccaaagta tttctagtat atgtttcaaa agttcttata cctctttcac aaagaactac
     7321 attttcattt cctccagcca ttatatactc tgctgacatt agccactctt caattgtagc
     7381 agataatcct ctttttaaaa gtattgtttt atttgtttta cctaattctt ttaataaatc
     7441 aaagttctgc atgtttctag ctccaacttg aattacatct acattttctt caaatatatc
     7501 tatatcttgt gttgacatta tttctgtaac tataggaagc cctgtttttt cttttgcttt
     7561 ttttaataaa tccaatccat catatttcaa tccttggaaa gcgtatggtg atgttcttgg
     7621 tttgaatgct ccacctctca aaaatcctgc acctgatttt ttcacatctt tagctataga
     7681 tactatttgg tcttctgttt caacagaaca aggcccagct atcatagcaa ttttctttga
     7741 accaatttcc attccattaa catttatgat tgaaggttct ggatgaaatt ttctattggc
     7801 ctttttaaaa ggctctacta catgcataac tttttcaact atcttatttg cttctattct
     7861 tttggcatct attttactag tatctccaac taaacctaaa acagttaatt ctgttccatc
     7921 tatgggatta acttctactc cttctctccc aatagcctca attaattttt taacttcatt
     7981 tttatctgct cccattttta atactactat catttttact acctcccaat attataattt
     8041 tatatttaaa ataaaaaaga gaactcacta atgagttctc taaaataatc tatcaagcac
     8101 taaatcttat ataaatatat aaaataatca tttttataaa atttacttat gctcaaaatc
     8161 actaaaataa ggcctcacca gaaaggaagt acatatattt ttgtaaaaga aatagccagc
     8221 gctaaaataa aagccccaga acataaagta aaaaaagcta taaagttttg ctgttagttc
     8281 ttttaatatt cctttcatag tattatcctc catttttata atttattatt cttcttgttt
     8341 aataattata acaaagaaaa tataattgtc aatattttct ataaatattt tcaatttaaa
     8401 atattttttt atttacttaa atcagcttct caattgatgt atttttataa aatcaattaa
     8461 acatcatctg taatttacaa taattttttt attttaaata ttataattcc aataaaaatt
     8521 atacatagca taaaaaataa agttgctgga tatccataaa tccagtttaa ttctggcata
     8581 tatctaaagt tcattccata tataccagtt ataattgtaa gtggtgctga tatggctgtt
     8641 aacaaggtta attttgtaat taagttattt gttttctcac ctactctgga agaataaata
     8701 tctaataatt tatctgctaa ctctcgtgtt gacaatgcga aattgtataa tttgtcaatt
     8761 ccaaaatcta taccttgaaa attttctaaa ttatattttt ttacatttga atattttaaa
     8821 tatcttatat tttccttaac tattctatcc ccaatataca aaagaggtct agtattctta
     8881 actataattc ttgttaatcc tcttacatca cttattctag atacatgatt cttattaatg
     8941 ttgtccatca tttcatcttc tatttgaaga attaactctt caactttttc caaagactca
     9001 aaaccatttt ttataacttc tctaagaatc aagtagttta tcttaaatag attaataact
     9061 ggattttttt caagttgaga gttcttcaaa ataatatttt ttacaaattc aaataaaaag
     9121 tgttcttcat tcactacaac caatataaaa ttatctgata aatacatatt tacttcttct
     9181 ataacagctt ctccatctct tagttcaaat gtatttaggc ttaaaaaatc gtaattatca
     9241 aataaatcaa gtttggttat ttcatcaaat ttaagacaat cattaaaagt tatttcatct
     9301 atatccaaag tttccttaag caattttaaa ttttttggag cacttaatat aagataagaa
     9361 ttttctgcta cataaaattt atctctaaaa tcttcaatag tctctcttgt atttaaattc
     9421 agtatataca tacatctact cctaaatttc aatttacatt ctaaaaattt tttataatcc
     9481 ttactgtaat tattttacac tattttaaca attattattt aatatttaca ttaaatttat
     9541 tataaggtaa gtgttaaata catttattat ttaaccaaag tttttattac agattacact
     9601 atatacttat tttaaaacat atttcatatt attgatacta aaaagctaaa aaataaaatg
     9661 catgtgctaa actgacaaca agcacataca cttcattctt attttaacta aactatataa
     9721 ttttaatata tattaataag cacataatct aataattaat atattacaac tattcatcat
     9781 ttaatctgta tcctacacca acttctgtaa ttatatatct tgggtttgca ggttgttttt
     9841 ctatttttcg tcttaatgtt gccataaaga cccttaaaga cttagtctca ttccctataa
     9901 cactacccca tatttctttt attataaaat tatgagtaag cacttttcca tgatgctttg
     9961 caagtaatga aagaatatta tattcaatag gtgtcaaatg gacttcaaca tcatcaacaa
    10021 taacttttct tttatcaaaa tcaatcaata atttatctac ttcaaacgta gatttaacat
    10081 catctttttg ttgagcttct acttgtgaat gtctaaatgc gactcttact cttgctaata
    10141 actctactat ggagaatggt tttgttaagt aatcatctgc ccctgcatca aatacttcta
    10201 ccttatctct atcttgctct ctagctgaaa ctacaattat tggtatattt gaaaattgtc
    10261 tgatagcttt tattacctct attccatcca tatcttctaa gcctaaatct aaaagaacaa
    10321 catctggaga atatgagaca gcaatagata taccttcttt tccagttata gcctccttga
    10381 catcataatc ctgtgttaaa agtgcagtag atataaattt tcttattgtc ttatcatcct
    10441 ctactaacaa tataagtttt ttactcattt cttcctccaa aactaatttt aaaatttaaa
    10501 actattcatt ttccttaggc aaactaaact taaaagttga acccttatct ttattgtttt
    10561 ctaccataat ttcaccttta tgagcttgaa ttatagattt acaaattgat aatccaagtc
    10621 caacaccctt tctggaatct ttagctcctt cttctccagt aaaaaatctg tcaaagatat
    10681 gctttttaag ttcctttgat ataccaggac cattatcaga tacttcaaac caaacataat
    10741 catctttctc gtatacattt atctctatct tacaatcttc ctttgaatat ttcaaagaat
    10801 tatctattaa attaattaaa acttgttcta ttaattttgc atccatagga acaaataata
    10861 tctgttctgg tatattaata tctataagtg cattttcaat tctttttttt attttttgta
    10921 acgcttctga tataatttcc tcaaccagtt cagaatgttt tttaacttcc aatttccctt
    10981 catctatctt ggtcatacta agtaaatttt caacaagtct tattaaccac tgagtatctt
    11041 catatacacc actaagtaat tcatctatta tatcttgacc tatagtaccc ttgtttttta
    11101 taattgtact tactgcacca gatattcctg ctagtggagt tctaaggtca tgcgaaactg
    11161 ctcttaaaag attacttctt aatctttccc tttcaatctc aagattggta ttttctttag
    11221 cttctgacaa gatttctcta tcaagcgcta ttgccatttg agcaattatt gtttcaatta
    11281 agcttatatc ttctgtatcc aattttgaat caatacatga tattcctact actcctaaag
    11341 ttaaattcat tcctacaatt ggtatatagt atccctttga accaggaagt gtatccgtgt
    11401 catttcctgc cactgttgaa ttggataaaa cccaacttgc aacagctttt tcatcttcac
    11461 tatccaaatc atttttatcc ttattattag tatttttttt gtaaagtttt gatttattat
    11521 gcttatctac atttaaataa caaattacat ctctacttaa gctaagagac aaaagttcta
    11581 tacctttgtt tactatatca tctttacttg ataatcttaa aaatccctta attatttgat
    11641 atatcatttt tgtattttct tctcttttag cagcagtttc tgcttcatgc tgtatcttag
    11701 acgtaagagt gctagttatt atacctacta ttgagaatac aaccagtgtt actatatagt
    11761 ttgaatctgc tatagataat gtatataatg gtgctgtaaa aaaataattt aaaagaacta
    11821 tattaaatac agaacttatt atacctgtac tatacccttt agtccacata gaaactaaaa
    11881 ctacacccaa catatatatt aacaatatat tttctcttat aaatcccatg ctttgtactg
    11941 catatgctaa aatacttatc atcaaagtaa taaatataag ttttataaaa tcaactttgc
    12001 taattttaaa tttagatttt attgatgatt tatccttttt aggtttgtat ttaacatctg
    12061 aggcaaattt atatggaatt atatgtatat ctatataatc aacttcatca attaatttgt
    12121 caactatatc ttttttaaat cttttactaa atcttttatt acttgaatga tttctaccta
    12181 ttacaatttt tgttacattt ctcagttttg caattctaag tatttgctct attatatttt
    12241 caccatgtaa aacaattatt tctccaccta atttcttagc taattttagg ttattttgta
    12301 gttgtttttg ttcatcaaat ggaagtcttg ctgtgtttgg agtttcaaca tataaagcta
    12361 tccattttgc aaatgtactg tctgatattc tggatgcagt tctaataact tttgctgaag
    12421 atggagatgg acttatacat gcaagaagtg tatctgatgt aggtattaca gttacatcac
    12481 cctttgatag tctggtcatt tgaacttctt tattaactct ctctgctgtt tttctaagtg
    12541 cgatttctct aagtgcaacc aggttatctt ttataaaaaa attatttttt gctctagtag
    12601 cttgttcttt cttatatatc ttaccttcat tgaacctttc aagaagtaca tcaggttcaa
    12661 catctataag ttctaactgt gtatttgtat ccaaaaactt atctggtata gtctctttaa
    12721 ctgatacgtt tgtaattacc tcaacaatgt catttagact ttctaaatgt tgtacattta
    12781 cagtagtgta gacatttatg cctgctaaca gcaattcttc tatatcttgc catctttttg
    12841 tatgccttaa cccagcaaca ttagaatggg caaattcatc tactaatata acttctggtt
    12901 ttcttgctaa agcagcatct aaatcaaatt cttttagttt tatagattta tattctatct
    12961 cctttacagg aagaatttcc aagccatcaa gtagagccaa tgtttctggt ctagtgtgtg
    13021 gttcaatata tccaactact acatctacac ccactttttt taaattttgt gcagattcca
    13081 acatagagta ggtttttcct actccagctg cataaccaaa gaatattttt aattgacctc
    13141 tacttagttt ttcttcttca tttttaattt tttctaatag aatatcagga ttaggtctat
    13201 ccattaaaaa acctccttac accattcatt actattataa tatcaattcc gatataaaat
    13261 tgtagttaag atgtattttt aatgattaag attatgtaaa gatttaaata tgaatttaaa
    13321 aaatttagtt gtaaaataaa aatgcctaac aataggcatt tttatcttgt gtataatcat
    13381 tttagtttaa gttaagtatt tccataaact catcacctgt aatagtttct ttttctaata
    13441 aataattagc aagttcatgc aatttatcaa tattattttc taagatttca attgcatttt
    13501 tgtgacaagc ctttactatt ttaaatacct cttcatctat tttagcagca gtgtcggaag
    13561 aacaagcaag agaagtatct cctcctaaat attgattgct tacagtttca agagcgacca
    13621 tatcaaactc ttcactcata ccaaggcgag taaccatagc tcttgcaatt ttagtagctt
    13681 gttctatatc atttgatgct cctgatgtac aagtgttaaa gataacctct tcagcagcac
    13741 gaccacctgt aaaagtagtt attttatcaa ttgcttcttc tttagacata agtacactct
    13801 catcttcagc aacttgcata gtatatccca atgctcctga agttctaggt attatagtaa
    13861 tcttatggac aggtgcagaa tgtttttgca tagcagcaac aagtgcatga cctatttcat
    13921 gatatgctat aattttcttt tccttatctg aaattacagc tcctttacgt tgatacccag
    13981 caattacagt ttcaacagat tcatcaaaat cctgttgttc tactatatct ctgccagact
    14041 taactgctaa aagtgctgct tcgtttacaa tatttgcaag ttctgcacct gatgctcctg
    14101 atgtagacct tgcaattgca ttataatcta tatctgagct tacatttaca ttttgagcat
    14161 gtacctttaa tatagcttca cgtcctccaa ggtctggaag ttcaactggt atacgtctat
    14221 caaatcttcc tggacgaagt aatgctttgt caagactgtc aggtctattt gtagcagcaa
    14281 gtattactac tccaattcca ccatcaaaac catccatctc acttaacaac tgatttaaag
    14341 tttgttcacg ttcatcattt ccacctaatc cacttccatt atcacgcttt ttaccaatag
    14401 tatcaatctc atctataaac actatacatg gagctttttc ttttgcttgt ttaaataaat
    14461 cacgaatcct agaagctccc attcctacaa acatctcaac aaactcagaa ccagatattg
    14521 agaaaaatgg tacatttgct tcacctgcta cagcttttgc aaggagtgtt tttcctgtac
    14581 ctggaggtcc tactaaaagt gctcctttag gcatttgtgc tcctatcttt ttatactttt
    14641 ctggattgtg taagaaatct actatttcag ttaaagcttc ttttgcctca tcttgaccag
    14701 ctacatcatt aaatcttttt ccactttgag cagatacata cattttggcg ttgctttttc
    14761 caaattgcat agcattacca cccattcgct tttgcataga cttcatcatt aagttaccaa
    14821 atagcataag aatacctata ggtattatcc atgtaagtaa aatacttaaa agaggtgaat
    14881 tttccttagg aataacctta gtaaattcta cttttgcatt ttttagtcta tctaccaatt
    14941 ctgggtcatc cattctacca gttatgtata tattcttatc cttgctacta gatggattta
    15001 ttgctattct attattttct atctcaacag atttaacctt tcctgtatcc aacattgtga
    15061 gaaactttcc atagtcaatc tcaaccattt ttttatccat taaacttgga aatattaaag
    15121 catttaaaaa cattactact attattataa ctgtataata atagagtgct gacctttttt
    15181 ggggtttatg ttcatccata attttattct cctttaaatt atttttatgt tatttgcaat
    15241 caatgtaagt taaaagttta tttaatttct ctaaaccttc tattatatct tccatatctt
    15301 gcaatttttc actggtcata ttttcaaaat aactatcaaa aatactactt gaaatatgtt
    15361 cttgtatatc caatgattta tttgtaactt ttacttttac aattcgttgg tcttctatat
    15421 ctcttatgcg atttaaaaag ccattttttt caagcctttg acagattact gatacattac
    15481 tatttgtcat gtttaagttc ttccctaaat cgcttatttt tttctctcca tgttcacata
    15541 actcagataa aatcaaagct tgcactgctg taataccaag tggctgataa aaatcactta
    15601 ataaagttgt aaatttcata tttatatctc tagtcaactc tagtagctct ttcttaaaac
    15661 tcatctcatc catacaaacg ctccctacga aaatacttta tttcaaattt aggctgaagt
    15721 gcatactgta aataaatact taatactata atgtttaatg tattaaatat tatagtatta
    15781 agtatttatt gtcaattaca atattgtaac ttcaattgga attttgttat cttattgata
    15841 tttaatttta tacaggatat ttttaataaa atttaataaa aaatcgatat atatatttta
    15901 cactgatttc tcccttttta atctctttta aaagttaaaa tacaaattta ttgatactat
    15961 aaaggtttag tctcccatta tcgcaccata tataaaatta aaaccacctc tatctacata
    16021 cacctaaagt gattttctta aaatataaaa ctactattat aaggttaaat ttaaaaaaaa
    16081 taaggtataa agtatcatta agattaactt tatacctata aatttctata tttttttagt
    16141 taacatttat atactcttaa aatttattgt atcaaatagt tttttatcca atttctaaac
    16201 aacaattctg aatgtttttt ccattttaca gttggctctt ctgaagggtc atcattaata
    16261 tagtaatttt gaggtataga tattttatct cctttattta tatccctctt atattcttta
    16321 tcaagagtgt atttgtcata ctcgctatga cccattacga acacatctct tgaatttgta
    16381 attatatagg gaccagcttc tttagaatga ctcacaatgc tcaactcttt attctctttc
    16441 aaagcttcaa tatttacagt agtatgtctt gaatgtggag caaaaaattc attttcaaat
    16501 ccatctacta ttttactatc tttatctacc ttatgcttaa atactccaaa acatttttga
    16561 cttagtggaa gtttttttac attgtagtat ttgtaaagac ttgcttgagc tgcccaacat
    16621 atatataatg tagatttagt ttttctctta gaataatcca taatttcttc tagttcattc
    16681 caataatcaa cttcttcaaa ctccatttgt tcaactggag ctcctgttat tataaagcca
    16741 tcaaaatact ctcctttggc ttcatcaaat gttttataaa attctttaac atattcatta
    16801 caacttcttt tactttctct tgttgaaatt ttaataaatt caacagacac gttgaatcct
    16861 gttttatcta tccttcttag taaatctaat tctgtatcta tctttatagg cattagattt
    16921 agtatagcta ttttagtgtc tatgttttct tcatacttta attccttttt aaaatcttct
    16981 ttataaatta catctattcc ttcatctaat aatttattaa taacaggtaa tccctttggt
    17041 agtattaaag ccatatcttt tcacttctct ttctcttaga atattagaaa tttaaagctt
    17101 gatttaaatc agctattaaa tcctctacat tttctattcc aacagataat cttattagtg
    17161 aaggcaatac tccagatgca attaattgtt cttctgttaa ttgtctatga gtagttgaag
    17221 ctggatgtat aacacaagtt cttacatctg aaacatgtgt aaccaaagat gctatctgta
    17281 atttttccac aaattttttg gcattttcta acccacctct tactccaaat gataaaacac
    17341 cactacatcc tcttgataaa tactttttgg cattctcata atacttgtta tcttcaagtc
    17401 ctgggtaatt aatccaatct acattttcat gtttttctaa aaatctagct attttaagtg
    17461 cattttcact atgtctctcc attctaagat gtagtgtttc aacatttaaa ttagtaagat
    17521 acgcattgaa tgggcttaaa caatttccat agtctctaag caactgaact cttgctttag
    17581 ttgcatatgc ggcatttcca aatttttgag tatagcttat tccatgatat gttgggtctg
    17641 gctcaactaa ttctggaaat tttccattat cccagttaaa gtttccacca tccactataa
    17701 ttcctccaac acttgaagca tgcccatcta aatattttgt ggtagaatga gtaactatat
    17761 ttgctccata ctttaaaggg ttacaaagca ctggagatgc taatgaatta tcaacaataa
    17821 atggaacatt aattctctta gctacatttg ctattttttc aaaatctatg acttcaagag
    17881 tcggatttgc aagtgtttct gcaaatacaa ccttagtatt ttcctttgca agttctacta
    17941 tctcatcttc acttgaatct aagtcaaacg atattaaatc tataccaaat tttttaagac
    18001 taggtcctag taaattgaat gttcctccat atacttttga agaacaaagt atactatctc
    18061 ctgatttaca tatatttaaa actgccaaca tatttgcaga ttgccctgat gatacagcta
    18121 cagaagatac tccaccctct agtaaactta ttttttcttc aaaagcttga atagtaggat
    18181 tgcttattct tgaatatata tgtccatcaa cttttaaatc aaataattca gcaagttggt
    18241 ctatactgct atatttaaaa gttgtacttt gatataaagg taatactctt ggttctccat
    18301 tacctggttt ataatttcct tgcacacata ttgtttcttt attatacatt cttgtatccc
    18361 ccttagattt tagtaaaaaa aattaaacta tttatcagat gagagttaaa ataaataaag
    18421 cctgagtata taactcaggc tctgtataag cttaattata ctcatctctc agctttcgcc
    18481 gcaggattta gcaccaatgt atataaatat actggttgcc gggtttcata gggccagtcc
    18541 ctccacctct ctggataaga tttatttaat ttttctatgt tttatatctt atatattttt
    18601 cctaattttg tcaactattt ttttcttttt tttcttttaa taattttccc aacctctact
    18661 tgaattacag acattatggc taaaacaatc gttataagcc actctaagtt atttaaaact
    18721 gttaagttaa aagcatttct aaaaacaggt acaaacagta ttactgctat taaaaatgca
    18781 gaagttaaaa atgagattaa aagaaatgga tttttaccat tatttctatt aaacattgaa
    18841 tctgtatttg aacgctgact aaatgctcgt aacatctgtg aaaatgctaa tacacaaaat
    18901 gccattgttt gacctacaga atgattttct atattaagac caattttata tgctaaaata
    18961 gtcattattg ccacaaatat accttgggta acaactcttt gtataagatt tttttcaaac
    19021 aaagtggttg atttcactgg ctggtgcttc attatatttc tgcttggtgg gtcaactcca
    19081 agagcaaggg ctggaagcgt agcagttgca aggtttaccc atagaatatg aacagcaaga
    19141 agcggagtat cccaatttaa taatattgct acaaacaacg taagtatctc tgctatatta
    19201 ccaacaagca aaaattgtat aactttttgt atgtttctat atacacgtct accttctttt
    19261 attgcataag caattgttgt aaaactatcg tccattaaaa tcatgtcaga agaatctttt
    19321 gcaacatctg taccacttat acccatggct ataccaatat cagcagactt taaggcagga
    19381 gagtcattta caccatcccc agtcattgct ggtacttctc caattctttt tagagattgt
    19441 ataattctaa gtttatcaaa cggggacaca cgagcaaata ctgtagtatt tttaacagct
    19501 tcatctagtt catcatcact aaaattatca agttcatcac cagatatcac agtattttct
    19561 tcactccata ttcctaactc atgagcgatt gttgtggcag taactttatg gtcacctgtt
    19621 atcattatgg ttcttatacc tgcttcttta caagtactta ctgcatcaat tacctcagtt
    19681 cttggtgggt caatcatacc aaccatacct ataaatgtca aatcatattc tatattttca
    19741 ctatcttctt ttggtataga tgaaatctca cgcttagcaa aaccaagtac acgcaatgca
    19801 tctttagaca tgttaagaca taattcgtgt atattctttt tatccacttc ggtaatgttt
    19861 ctttcacctt ttgaagttaa tattttagta cataggtcga gaatttcgtc tactgctcct
    19921 ttagtataag caataatctt ttcatctata ttatgaacag ttgtcataca ttttctatct
    19981 gaatcaaaag gctgttcaaa tacacgtgga tattcttctt caagagcttc ttgatctatt
    20041 ccaaattttt tggccataaa aattaatgcg ccctctgttg ggtcacctaa tatttcatct
    20101 gttttatctg gattaataca tgcatcatta caaagtgcac cagcataaac aatctcttta
    20161 tacactaatg gatgtttttc agatgcagta tctattgatg tcacttttcc agattcaaaa
    20221 tctccattga cagcaatatg tgtaacagtc attttattta aggttaatgt accagtttta
    20281 tcacaacata ttactgtagc tcctcctaat gtttcaacag caggtaattt tctaattaaa
    20341 gcatttcttt tagccatacg ctgtacgcct aaagccatta caatagtagc tgttgcaggt
    20401 aatccctctg gaataattga tatggcaagg gaaattgcaa tcataaacat tggcaagaga
    20461 ggtctctgat aaaatgcacc tattgcaaat ataacaacgc atacaataat acctatgaca
    20521 cttaatgtct ttccaacagt attaagttta cgttttatag gagtatcaaa atcatcttgt
    20581 gaatctaaaa gatgtgctat gttacccact tctgtatcca taccagttgc tactacaaca
    20641 cctacacctc taccatatgt gactattgaa gatgtatacg ccatattgct acggtctcct
    20701 aatacacatt ctttaggtaa gattgtatct gcatcttttt ctgatggaac agattctcct
    20761 gttaaagaag cctcttgaat ttttaaactt gatgtttcta ttaaacgaag gtctgctgga
    20821 accatagctc catcttctaa aaaaacaata tctcctacta caagattact tgctggaata
    20881 acattttctt caccatcacg cataacacga gctgtaggtg agttcatatt ttttaatgct
    20941 tctaatgatg attctgcttt tttctcttga acaattccaa taactgcatt tactgttaca
    21001 attattagta ttacaaaagc ttctgtaaat tcgccaaata tcaaagataa tattgctgct
    21061 cctattagta tcataaccat agcatctgta atttgactcc aaagcatttg taaagttgtt
    21121 ctaggtggtt tttttctaag ttcatttaag ccattctctt taagccgttt ttccgcctcg
    21181 gtatcggtga gcccctcctc tgatgaatct agactttcta aaatttcatg cgttgctctt
    21241 gaatgccaag gcatctgttt gtgattttcc attttggaag accactcctt tcttttttta
    21301 atttaagaat aattatattt taaaatcaag ttcaattcaa tacacactaa gtatatttta
    21361 cctaaattta acaatgccat ttaatattta acaaaacttc tactctttta caaatatgta
    21421 ttcactattg ttagataagt tttctttata cttataacct tctaaattaa actttttcag
    21481 ttcttcaata tctcttatcc tattttttac aatgtatttt gtcatcatac ctctagcttt
    21541 ctttgcttgt gtagattcca ctttcaatat gccattttta tttactttaa aagtacatgt
    21601 aatatatgta tcactatctt taataaattt ttctatggac ttgctgtatt catttgatga
    21661 aagattaatg atagctgttt tttctttgat tagttcttta tatatacgat ttccccaata
    21721 ttcgtataaa ttcttaaact cacctactcg taattttgtc tgcatttcta atctatattc
    21781 atatatacta tcatatggtt ttacaacacc atacaatcca cttatgattc gaatacagct
    21841 atttgcaaac tcttcatctt tccttgtaaa attttctgct tctatatttt tatattgtat
    21901 tccatcataa gttaagattg cagggtttcc taatctatca aatttaatgc tttcaaatct
    21961 atttttattt aatacagcta atttttcatt aattttcatt ttatttttaa aatcttctat
    22021 accaaatgtc tttatatttt ccactatttc ttttgtgtta tctataaaac aaggcaaact
    22081 caaatctcta tcaaatactt ctatattctt catattttta gctggtgata acattataat
    22141 cataaattct tattactcct gtttttccaa atattcaaga atctcttgtg catttgtgtc
    22201 tggttttgct ttttcaaaga ctttttcaat aattccattt tcatctataa tatatgtcgc
    22261 acgtacaact cccaatgcag tttttccata catttttttc tctttccata catcaaaagc
    22321 ttttatagca actaagtcag ggtcagaaag taaaataaaa ggaagttcat gcttttctgc
    22381 aaatttttga tgtgatttta tactatcttt acttatccca ataacttgta tatcttcctt
    22441 tttaaatcca tcatatgcat ttctaaaagc acaagcttga cgtgtacatc ctggtgtatt
    22501 atctttagga taaaaatata ctactacttt tttgccttta aaatctgaca tacttacctt
    22561 atttccatct ttatcttcta gtgtaaattc tggtgctttt gttcctatac tcaacataaa
    22621 tatctctcct tttgttttta ttaataattt tatactatgc
//
LOCUS       DAMJVX010000044        22564 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMJVX010000044 DAMJVX010000000
VERSION     DAMJVX010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22564
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.046"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(29..385)
                     /locus_tag="PC407_001604"
     CDS             complement(29..385)
                     /locus_tag="PC407_001604"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2497056.1"
                     /translation="MVFETFDKVIKLHPNAKPVFHSDRGFQYTSKVFESKLLAQGMIQ
                     SMSRAGRCIDNAPMEGIWGILKVEMYYLRKFDTYEQLVKIIETYIYYYNNFRYQKRLN
                     SMSPLEFRKYLSTEIA"
     gene            481..974
                     /locus_tag="PC407_001605"
                     /pseudo
     CDS             481..974
                     /locus_tag="PC407_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1516..2871
                     /locus_tag="PC407_001606"
     CDS             1516..2871
                     /locus_tag="PC407_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2497057.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATVLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(2989..3657)
                     /locus_tag="PC407_001607"
     CDS             complement(2989..3657)
                     /locus_tag="PC407_001607"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HDF2497058.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(3724..4152)
                     /locus_tag="PC407_001608"
     CDS             complement(3724..4152)
                     /locus_tag="PC407_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2497059.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            4259..4852
                     /locus_tag="PC407_001609"
     CDS             4259..4852
                     /locus_tag="PC407_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDF2497060.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENATKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            4972..5907
                     /locus_tag="PC407_001610"
     CDS             4972..5907
                     /locus_tag="PC407_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDF2497061.1"
                     /translation="MDKFKYAFDDKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(6178..7122)
                     /locus_tag="PC407_001611"
     CDS             complement(6178..7122)
                     /locus_tag="PC407_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2497062.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKVCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            7438..8868
                     /locus_tag="PC407_001612"
     CDS             7438..8868
                     /locus_tag="PC407_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2497063.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNADLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            9068..9652
                     /locus_tag="PC407_001613"
     CDS             9068..9652
                     /locus_tag="PC407_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HDF2497064.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(9756..10535)
                     /locus_tag="PC407_001614"
     CDS             complement(9756..10535)
                     /locus_tag="PC407_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2497065.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(10605..11861)
                     /locus_tag="PC407_001615"
     CDS             complement(10605..11861)
                     /locus_tag="PC407_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDF2497066.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            12119..13018
                     /locus_tag="PC407_001616"
     CDS             12119..13018
                     /locus_tag="PC407_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDF2497067.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            13042..14490
                     /gene="ddlR"
                     /locus_tag="PC407_001617"
     CDS             13042..14490
                     /gene="ddlR"
                     /locus_tag="PC407_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HDF2497068.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            14549..15355
                     /locus_tag="PC407_001618"
     CDS             14549..15355
                     /locus_tag="PC407_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2497069.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKISTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            15381..17300
                     /locus_tag="PC407_001619"
     CDS             15381..17300
                     /locus_tag="PC407_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2497070.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVKRGAQ
                     ARSTKQKARLQRFDELMNRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(17542..17889)
                     /locus_tag="PC407_001620"
     CDS             complement(17542..17889)
                     /locus_tag="PC407_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HDF2497071.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(17876..19804)
                     /locus_tag="PC407_001621"
     CDS             complement(17876..19804)
                     /locus_tag="PC407_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HDF2497072.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTTLPIVLSKVKNNEQE"
     gene            complement(20215..22404)
                     /locus_tag="PC407_001622"
     CDS             complement(20215..22404)
                     /locus_tag="PC407_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDF2497073.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEYNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKKALPE"
BASE COUNT         8254 a         3370 c         2874 g         8066 t
ORIGIN      
        1 aaattttttt agagctactt aaaataaatt atgctatctc tgtacttaaa tatttcctaa
       61 attcaagtgg agacatagaa tttaatctct tttgatatct aaaattatta tagtaatata
      121 tataagtctc tattatttta actaattgtt catatgtatc aaatttcctt aaatagtaca
      181 tttccacctt taaaattccc caaattcctt ccataggagc attgtcaata caacgaccag
      241 ctcttgacat actttgtatc ataccttgtg ctaaaagttt cgattcaaac accttactag
      301 tatactgaaa acctctatca ctatgaaaaa caggttttgc atttggatgt agttttatta
      361 ctttatcaaa agtttcgaat accaagttat tattattcga ttttccaata acatatgaaa
      421 ttaatagata taacgccttc tattttacag tcttatataa ataacatata caatgagcac
      481 atgttaaatt caataaaaaa ccaagtcgat tttataaaat cagtattgaa agaatcttat
      541 agattaaaag aaatatcaga aaatatttgt gattttgtta caaccccaat caaaaaaaat
      601 agttcaactt ctaagtttta tacaaagcaa gaaactcaat tattattaga aaaatcaaaa
      661 aatattccta taggtatacc aatttttttg atactaactc ttggattacg ttttggtgaa
      721 gctgtatcac ttatatggtc agatgtagac cttgataaaa aatctcacat gtaaatcaaa
      781 cattagttta tgtatacaat aaaataatct tcaaagaccc taaaatccct agatccaaaa
      841 gaaaattatt tgctcctgat gaacttataa gtttgcttaa agggcaaaaa ttaaaacaaa
      901 ataagttaaa attacagggt atattgaaaa atgaatttga tttgatatgc ttaaataaaa
      961 gattcaaccc gtgagttcaa ccaacttttg ttgttcattt taaaaaacta ttaagagata
     1021 ataatcctag agatataaaa gtacatgaat taatacatgc aaatgcgact ttgatattac
     1081 tttcaggtac taacataaaa gctatttata aaatattagt acatgcagat ataaaaatag
     1141 ctatgaataa atattcgata ttctcactta ttagatgaaa tggataaaga aacatctgaa
     1201 aatataagca aaatactatt caaataatgt tcagttttat gtcaattaaa tgtcactcaa
     1261 agtgcttttt ttccatttgt cagttaaatg tcacttaaag tacctttttc ctatttgtca
     1321 gttaaatgtc agtaatatat aaataagata tatttataag acccttaaaa tcactattat
     1381 ttaataaaat ttataattat atactatttg ctgttgacat tggtattata ccatagttta
     1441 tcatttatat tagaaataaa attattaatt aatagtttcg taattttata atagatttac
     1501 agggagtgat aataaatgga tacaaaaata tctataaacg aaaacatgag tctaggtaaa
     1561 agatttttta aatacctagc accatctgta gttgcaatgt gggttttctc actatataca
     1621 atggttgatg gaatatttgt aagtaaagga gttggagaat tggctttagc tgctgtcaat
     1681 atatcaatgc cttttattaa ttttatattt gcagtttctc tattattttc aactggtgca
     1741 tctaccataa ttgcaatcta tctggggaaa aaagatatta aatctgctaa cgaagtcttt
     1801 tcatttaact tagtatcaat aattatatta tcaataatta tattagctat aacttttttt
     1861 aatttagata gattagcact atttttagga gctacagaat ctacaattgg tatggttaaa
     1921 gattatttag gaataataat attttttaac ggattcttta tagtgtctta ttctttagaa
     1981 gttataataa aaactgatgg ctttccaata ttagctactg ttggtgtaat aatatctgct
     2041 cttacaaaca tcatacttga ttacttgttt gtaattgaat ttggatgggg tgtaaaaggt
     2101 gctggtattg caactgtttt gtctcaagtc ttttctacta ttttcttctt aatacatttt
     2161 ttaagaaaaa actctacttt gaatttttca aaatttagaa ttgattttaa gacacttaga
     2221 aaaattgtat ttataggttt tcctgattca actactgaat taagttgtgg tatagttgtt
     2281 ttacttttta acttaagtct tactaaatat attggagaaa atgctcttat ttattatagt
     2341 gtcataaatt atataaatac attagtactt atgactatga tgggtataac tcaaggtatg
     2401 caaccactta ctagctttta ttatggtgca ggaaatatag ataatgtaaa aaaattatta
     2461 aaaatgggga taaaagccac aataatagca tcagttgctg tatttgcaat ttgtatggct
     2521 ttttcaggac caatagtctc tttatttata cacccagaag aaacaatgct attcaatgaa
     2581 ggagttcgtg tatttaaaat attctctata tctttcttat tggtaggaat taatgtaata
     2641 atttctggat tctttgtatc agtagaaaaa ccatcaattt ctactgttat atctcttggt
     2701 agagggcttg taatagttgt tttaagtcta atttcaatga tactaatatt tggtggacaa
     2761 ggaatatgga tgactactat tgtttctgag tttatatgtc ttattttatc attagttttt
     2821 ctaaaaaaga atttttctac attagactct aatctaaata aggttgctta gatgataaga
     2881 gtagcaatat atgaaaatat ctaaataaaa aatacctatt atacaatctt gcaaatatta
     2941 gacctttgca aaaattagga ttatataata ggtattttat ttatgtaatt attttatttc
     3001 taaatatcca tagccatctt ttattaaagt tccttttact tcatattcta caggtttttt
     3061 aaatattgga ccatctacga caaatgtatg atactctcca tgttctccac atgggtctgc
     3121 accagcattt tttatcttct ccacaagttc tctagttaat ttttttccta aaaattcctc
     3181 tcctaagtgc tttaagttta cttttgttac aacactacaa aatccactgt ctataaattc
     3241 atatactaaa tcctctctac tctcttgcca taatggtaaa tctactttca taccaactgc
     3301 ttcacatcta tcagtatccc attttctatg actttctata tctatatctc catatgcaca
     3361 tatcgttgct cccatgtttt tagcttttat taaagccctc tcaaagctct cttcatatcc
     3421 ttctacacca cactcagcaa ctaataaagg taatcctata ttgctactta cttgcttaag
     3481 catattatat tctaaattat gtgtccatga ctttccatgt tcttcactta tagttgtcaa
     3541 gagtgccaca ggcttatatc cttttttcaa cattcgattt aatgctaata tactatcctt
     3601 accaccacta aatgacatta caaatttttc atcagccaat tttaacacct ctttcatcta
     3661 actttgtttt attaacataa ttataccaaa tttttgagtt gcatagcaaa ttatattaaa
     3721 ttgttaattc actataaaga atgtccctaa tattaaaagt ataaccccta tgtatttgta
     3781 taaatgcatt gtttcttgtc ccaaaattac gctatcaata aaaaatccaa ctaaaacttg
     3841 ggatacaagt gttaatataa gtgtatttga tactcctaaa tttggaattg cctttactgt
     3901 aaaatatatt attaaacctc caaatactcc tcctaataac aacttataat taaaaccatt
     3961 tatagatact agacacttta agtcacctgt aactattata cttgccaaaa aaaatattgc
     4021 tcctacagtt aaacttataa aggtggccac taatgctgtt gtactttttc ctagttctcc
     4081 atttataaaa gcttcaagag ctgtagaacc acctgctaat aatgcaaata ttattgccca
     4141 taattttgtc atggacaaac ctccttctat ataattctat taatagaagt ctatgataca
     4201 acctattaat taattactaa gtatagttta atttttatac actaggcttt atttggatat
     4261 gaatttctgt tataattgtt ctatcgattt tggaggtgat tttatgtaca caaattttaa
     4321 tatcgatttt aataacttta ataaaaaaga aaatgctact aaatttatgt taatgggagt
     4381 attgctcatt atcttaggat tattatgcct tacatttaag acattaggta taaaattaat
     4441 ttcttggact tttggtattg cacttttatt ctttgcatat ttaaatttaa aaaatattaa
     4501 tgaactaaaa agatatgcta ctaaagaaga aattaaaccc tctattaata tccaatgggt
     4561 tctaataatt gcatgtatat tactttttgt atttcctcaa aaaatacaaa gcatattttc
     4621 attattttta gggttttatt taatctttaa tcaactagta gcacttgtaa atagtaagaa
     4681 taatccttat tctaaattta caacttggaa tatagttaag attctatttg gtatatgcct
     4741 tatattatct ccactttttt tatctagatt tatagtatcg ataatgtctt ttttcataat
     4801 attatttgga ctagttctat ttttctctgg aaatacagcg agaaaatatt aattattttc
     4861 tcgctgtatt tttatattac ttaaaataga atttgtttaa gttatctatt tgtggtaata
     4921 tgttttttgt acaagctcag cgtacaattt tattagaaat aggtgatttt tttggataaa
     4981 tttaaatatg cttttgatga taaaagatat catacttgga attactatct tagaaatact
     5041 tttggagaaa aagtttttaa ggtatctata aatgcaggat ttacctgccc aaacattgat
     5101 ggaagtcttg gttatggcgg atgtacctac tgtagcaaag aaggctcagg tgattttgct
     5161 ggaaacccta aagataatct tatcagtcaa ttttataata taaaagaaat gatgttaaaa
     5221 aaatggcctc atgcaaagta tattggctac tttcaagcct atacaaatac gtatgctcct
     5281 cttgaagttt taaaagaaaa atatgagact atattagaac ttgaagatgt cataggtctt
     5341 tcaatatcca caagacctga ctgtttgcca gatgatgttg tagaatactt atctgaacta
     5401 aataaaagaa ccaatttatg ggttgaactt ggacttcaaa caattcatga ttctacatct
     5461 aaaattataa atagaggtca tgattataaa acatttgttg aaggtgttga aaaattaaag
     5521 tctaaaaata taaaggttgt cgtacacatt ataaatggtc ttcctggtga agattataat
     5581 atgatgatgg aaacagcaaa ggcagttggt aaaatgggtg tagacggtat aaaaatccat
     5641 ctattgcatg ttataaaaga tactcctatg gaaaagatgt tacaaaatgg tatgttaact
     5701 cttatggaac aagatgaata tatcaatctt gtctgtgacc aattagaaat acttcctgaa
     5761 actatgataa tccatagact cactggtgat ggtaagaggg atgaactagt aggtcctata
     5821 tggagcctaa aaaaatggga aattttaaat caaattgatg atactttaaa ggctagaaat
     5881 tcttatcagg gctgtaagtt tgtttaatac aaaataataa ctatagaaat ggttatataa
     5941 atatagtcct ttctatagtt atttagtatt tacaaaacaa aatattaatt tgtacattta
     6001 tacaatatat aataaaaaat agatagtcta aactgtatct acttatattt tatttgtact
     6061 gtccacttta ttctataaaa tttataaata gttatttttt aataccaaat ataatttggt
     6121 ataatttttg ttggattcta aaatgtatac caataaatcc tttttaagta tataattcta
     6181 aatactttca gttttttcaa tctgctttaa cttgttggat ttataaaata ttcttatttc
     6241 atcataagaa agattaagtg ctactactgc agatattaac atcaacatca ctccaaataa
     6301 cttccctaag gaaaaacttt ctcctaatat agtgcaacca attattactg aaccaactaa
     6361 ctctactgat gccacaacac ctgcaactga caactctaca cctttagata tacctgctgc
     6421 ataaaaaata tatgcaagtg cagctggaac aatgcctaac attagcatac aagataaaac
     6481 atctaaatcc ttagcataat ttaagatttc ccaaggtctc gaagaaggaa tcataaatat
     6541 agctccaaat aagaaactgt atactaatat tgttgaactt gaaaattcct ttaaagcatt
     6601 tttactaata gttggcataa gtgcatatgc tattgctgat aataccccaa gtaaaagtcc
     6661 aaaagcgttt aaaccttgaa aatccaaacg tccaccagtc actgccataa ttgcaccaat
     6721 aaaacaaaga ataagtgaaa ataatttgct tctagtaata ttttctttat aacatacttt
     6781 tgaaaatatt gctaaaaaca gtggtgatgt atatagtaaa actgctgcaa ttgatacacc
     6841 tgcaatgtca attgctttaa aataacataa attaaacatt gcctgacata taattcctat
     6901 tattactgaa tatataattc cttttttgct tatctttaag atttgtggtg tccttataga
     6961 tgaatatacg cctaaaacta taaatccaaa aaacaatctt gtaaatgcta cttgttctga
     7021 tgtcagtcca tttcccatta aaaactttcc aaataatcca agtgtagccc ataataaccc
     7081 tgctattgct ataaatacat accctttact tttttctccc attttaaatt cccttctatt
     7141 tttatttagt caatttacac tcttcttttt aagcaaaaat tatgccatct taaaccaata
     7201 tatatcgact ctaaaaccct agtctttcca ataatactaa taagttttca attttcttat
     7261 atttattttt cgcttacttt aagttgattt agacttattt taattgtaaa atgagttata
     7321 tataacttat ttaaagttaa aatagactga aacttatata aatatcaaat tttaagtata
     7381 ttaatcaatt aaacctattt taatatatca ttaaaaattt aggaggaaaa tataaaaatg
     7441 aatttagatt tggaatttta tcaaaaaata ttagaagctt cccatgatga aatatgtgtt
     7501 tctgatgata aaggaataat aatatattgt aataaagcat ttgaagaaaa ttatggtcta
     7561 aaaaaagaag atattttagg aaaaaatgtt tcttttttag aagactctgg atattcaaca
     7621 aaaagcccca taccaattgt attaaaaaca aagactaaat tttcactgga acaagatact
     7681 caaacaggga aaaagcttat cataactgct acccctatat ttgatgaaaa tggaaactta
     7741 gaatttactg ttgaaaattg tagagatata actgaactta ataatataaa aaataaactt
     7801 gaagacacaa aaaagcaagt aaaaaaatat aaatctgagg tagaaacact atacagaact
     7861 gcacttagaa ttgaagatac tgtaattatg gacggaatag tgatgagacc aatcataaac
     7921 acagtaaatc atgtttctaa aacggatgta agtgtacttt tattgggtga atctggaaca
     7981 ggaaaaagct cacttgctag atatatacac cacaacagca atagatctaa tggcccattt
     8041 ataacaataa attgtgcaac tatatctcca caacttcttg aatctgaact ttttggatat
     8101 acttcaggtg cttttacagg tgcaagtact aaaggtaaag ttgggctcgt agaattagct
     8161 aatggaggaa ctcttttttt agatgaaata ggagatatac cacaaaatct acaggcaaaa
     8221 tttttgcaat taatacagga tagaactttt actccagttg ggagcttaaa aaataaaaag
     8281 gttgatataa gaattatatc tgctacaaat gctgatttag tttcaaaagt aaaagaaaaa
     8341 aagtttagag aagatttata ctatagatta aatgttatag aaataaaatt gcctcctctt
     8401 agagaaagaa gggataatct agtggaaatt ataaaatatt attttaatag gtactcaagt
     8461 gacttcaacc taaataaaac tatttctaaa gaagctatgg atgctattgc aaattataga
     8521 ttcccaggca atataagaga attacaaaat ataattcaaa aaatcttgct tacttgtaca
     8581 gataatcata taacaataga tgacttacca aacattctta caaaaaatat acatattacc
     8641 aataatggaa ataaaacaca tatatcccaa attaataagg ttattatatc tgactcaaaa
     8701 tctactaatt ataaaaataa aaattttgac accttaatta aagagtatga aaaaaatata
     8761 attttggatg cttatgaaaa atttggtagt tcttataaag tagccaaaca ccttgaaatc
     8821 tcacagtcta aagccaatag gttaattaga aagtatacta atacttaatt ttaaatttga
     8881 taaataaaaa cttaacacta tttacatatt gtaaatttgg ccatttactg ttattatgaa
     8941 tatggttgaa attatttaaa taaatactgt tgacttagtt tcatatattt accattaaac
     9001 ttttatcata aatatcatat cataataata tatttaataa gtctaataaa ctatacgagg
     9061 tgtaaatatg ttaatgaata cccattttat tatggcaaaa tcaatacttg ataatataga
     9121 tgaaaataaa acattcttta taagcgaaaa aaattttata tatggcaata taaagcctga
     9181 tgctttttct aaatataaat taaaaaaaca ttatttagat gagtcattta atatgattgt
     9241 ttctaaaata aactatctat gcagtcttaa tatagatagt ctttctaaat acttttcagt
     9301 aagtagactt agtcaggaac taggagtcat atgtcatttt ttatgtgatt ttttctgtgt
     9361 agctcacagt gaacgttggg aatttaagca tagcttaaat aaacatgtta cttatgaaag
     9421 agaacttgga gcatttgcaa aagatgttga tttatctaaa attaaatgtg gtactataaa
     9481 tagtagtttt gagaagtttt ttactaaact ttatagtgat tacaaaaaga aaactgatta
     9541 tatgaatgat ttaaaatttt cagcttatac ttgtaatagt gtcataaatt atatacttga
     9601 ctctatttta tctaatacaa taaaatcata tcatatagca aattgcggtt aacttaagaa
     9661 tttttaaatt tatataaact tataattgaa tataattaaa atgactgctc tatagaatat
     9721 aattttatac ttctctagga cagtcatttt ttatactaac tatttatctc cattataaca
     9781 tctatggcac tttttacact atctactaca taatttgcac tttgctttac atcttccctt
     9841 gccttagaca ttgcaaaact atatttaaat ccttctaaca tgcaaatatc atttggagaa
     9901 tccccaatag tagcaacttc atctggactt acatttaacg tttttattac atgctctata
     9961 cttcctcttt ttgacacact tttaggaact atatttaaag tatatgttcc agaaaaatat
    10021 atttctaata cttcactaaa ctcacttttt agataaagtt ctattctgga taactcttcc
    10081 aaattttcag aacatacgcc tatatttcct ataagattat caaaactact tcttttttca
    10141 aaatctgggt caactataaa gtcactagaa taatgtctaa aagcagtggt atcactaccc
    10201 acaattacat cacccttata tttaaaataa agttgtgcat cacttgattc aaatctatca
    10261 atgacttttt ttactaaatt atcatctatc gttcttttaa aaattacatt ataatcttta
    10321 tcatatataa atgaaccatt ttcacataca taatatccat tcatatctaa actatctttg
    10381 ataatattat gtgcttgttt aaaaattcta cctgtagcaa cattaaactg tactcccttt
    10441 ttttctattt cttcaattgc tactatatct tcttttgtta tggttccatt ttcatataga
    10501 gttccatcta aatcacaaaa tatatgtttt atcataaatt aaaagcctcc aaaaacaatt
    10561 ttatattata gtaacacttc aaatatatcc aatatcataa caacttaaag cttttctaat
    10621 tgtattctca attcattaag taaatctcta gtagctctta atcttatctt tcttctatcc
    10681 ccattaaata tatttttctt tactattgtt tttccattta tatatattgc agtataaact
    10741 agtcccacag gcttctcttt ggttcctcca ccaggtccag ctactccagt tgtggatata
    10801 cctatattag ctttaagtcc tttagcaacg ccctcagcca tttctatagc tgtttgctca
    10861 cttactgctc caaactcttc caaagtttct ctttttactc ctaatctttt cattttagaa
    10921 tcattagaat atgttacaca tccttcaaga aatacagaag atattcctgg atagtttata
    10981 agagatgaag aaaccaaacc tccagtacaa gattcagcca ctgctattgt tagatttttt
    11041 tctacaagta acttagaaac agcatcttct aaagcagtct ccccttcaga tatggaaata
    11101 tctccttcag tatatacata tttaccaact ctacttttta ttttttcttc tactggtttt
    11161 attaatttaa aagcttctct ttcattttca gcctttgcag ttattcttat tgtaacttct
    11221 aattcttttg catataaagc aactgttgga ttagtctgct cattaattat atcgagaatt
    11281 tctaattcta gatttgactc acctattcca tataatctta aagtcttaga tactagcatt
    11341 tcatttgtga attgttgtaa gtaaggtttg acactctcat taaacatagc tttcatttct
    11401 cttggaggtc caggcaatac tattataaat ttaccatctt tttttaaaat tgcccctgga
    11461 gctgtaccat tattattttt tagtactata gcatcttttg gaaagtacac ttgtttcata
    11521 ttattttcgg ccattttttt tcctgtttta acaaagaaag attccaaaac ctctagtgaa
    11581 ggtttatgta gttctaattt ctgaccaaag tattctgcag cagtttcttt tgtcatatca
    11641 tcaccagttg gcccaagacc acctgtagta attacaaaat cacttctctt taagctttca
    11701 tcaaaacatt ctaaaagtct ttgtttgtta tctccaacag tactctgatg gtatacttct
    11761 attcccaatg atgcaagttc tttagctaaa aattgagaat ttgtatttac tatatctccc
    11821 aataatattt ctgttcctac acttattatt tcagctttca aaatttatca ctccttgatt
    11881 tatagccatt aagactagtc ttaagttcat aacaataatt ttagcaaaaa taataacata
    11941 aataaagtta aaaaatcatt taaacatatc tttaaactag attattattc taaaaccaca
    12001 cataatataa acttcgtata tagtggtact ttcatcctca actgttaatt catataatta
    12061 agtatgtaaa taaaaagttt actttaaaat aataaaaatt aaaataggag tgtataatat
    12121 gaaaatagca gttataatgg gtggaatatc atcagagaga gaagtttctt taaattcagg
    12181 aaaagagata tataataacc ttgataaaaa taaatatgaa gttgtaaaag taataataga
    12241 tgataaaaaa gatatattta caaagatacc agaagatata gattttgcaa tacttgcttt
    12301 acatggaaaa tttggtgaag atggatgtat tcaatcaata cttgagacta tggatatacc
    12361 atactctgga tgtggacctc tttgcagtgg gatgtgtatg gataaaaata taactaaaaa
    12421 aatgcttaga gattcaaacc tacctactgc tccatgggtt ttagtaaaat cagttgatga
    12481 aatcgactat gatgaaatag ataatatagg ttatccagta tttataaaac caaactcagg
    12541 aggttcaagt gtagccacat tctttattca ttctaaggat gaagttgaag aagcagttag
    12601 aaaaggtctg gaagttgacg aatttgtaat gattgaaaaa tatataccag gtggagaata
    12661 tacttcattc atattaaatg gagaagtgtt ccctacaata tctataaaat ctgattcagg
    12721 attctttgat tatgaagcta aatactcagt tgaaaaaggt gcaaaggaag aagttgttta
    12781 cttagatgaa gaacttcaaa aaagagtaaa cgaaatttct gagacttgtt ggaaaatttt
    12841 taattgcaaa gcatatgtaa gagttgatat gattataagt gaaggtattc catatgttct
    12901 tgaactaaat actcttcctg gaatgactca aactagttta attcctagaa gtgctgctgc
    12961 tagaggtatt aagtacagtg aactattaga taaacttata gaatactctt taaattagat
    13021 gaaataattt gaggtggtaa tttgatattt tcaaatctaa aactaaatga taatgaacca
    13081 atttatatac aacttaaaaa ttatataagt gatatgatat caaaaggact aattccagac
    13141 aatagtaagc tcccttctac aagggagctt agtcagcttt tacaagttag ccgaaattct
    13201 gtagttttaa cttatgaaga attaaaatca gaaggattaa tctactctat ttcaggaaaa
    13261 ggtacatttg ttaaatcaaa gaataagtct tcaaatacta catggtcatt aaactgggat
    13321 tgtttagaga atacatactc taaaaaagcc aatgaattgg acattataaa aagtgaaatt
    13381 ccttggagct ctgacctgat ttcctttaaa agtatttctc cagatggaga tttattcgac
    13441 atggaagaat taaaaaaatc ctttttaaat agaatttcac tagaaggaca taaattactc
    13501 aactatggat atgcacaagg ttataagccc cttattgact atttactaga atatatgaca
    13561 aacaagggtg ctgatataag taataaagat atactaataa caaatggatt cacagaaggc
    13621 tttgatatta ttatgtcatc ttttacccaa gaaaaagact acataatatg tgaaaatcct
    13681 actcataata ctgctataaa aattatgaaa tctcataacc tcaatatctt aggtgtagat
    13741 ataaatgaag atggtttgga cttcaatatg ctagaagaca atttagttaa gtataaaaat
    13801 aaaataaagt ttgcatacat tacaccctct tatcacaatc ctacaggaat tgtaatgact
    13861 cctgaaaata ggtacaaatt ctataatctt atgaaaaaat acaatatagc aattatagaa
    13921 gatggattta atgaagaatt actctacaat agttctcata tatttcctat ctgttcccta
    13981 gataatatga ataatggggt cgtatatatt ggtagttttt caaaaatttt atttcctgga
    14041 atgagaattg gatggatatt tgcagataaa aaagtaatca ataaactaga aagtgtaaaa
    14101 aggtgcagaa acatacatgt atccttttta gaccaaggaa ttttatttga ttatttaagt
    14161 aatggaggtt ttgaaaaata cattaaaaaa atacgtaaat tctatgggga caaatttaac
    14221 tttgcatata aatgtataaa aaaatatata aaaacagaat atatcttggg tgatggaggt
    14281 ttgcatatat ttataaaact aaaaaatata gatactagag ttttattaga aaaatgttat
    14341 gaaaaaaatg ttatatttat gcctggagat ttatttttta cagataactc tggttttgat
    14401 actttaagac tcggattttc taggttgtct tttgaagaca ttgaaattgg aataaaaata
    14461 attggagaaa ctataaatga aatgttctag gttaactaaa tattgttagc ctagtttatt
    14521 tttatgaggt ataatatata tagtttatat gaatctaata ctgggggata atatgagtaa
    14581 aaagtcaaaa cgaagaaaaa ttaataaatt aaaattaacg attgtaataa ctgtattatt
    14641 tcttgcttta ttatctatat atgagatact gttctctaat cccaacaaaa aaggtaatat
    14701 aaagagtcaa gaaactatgc agaataaaaa taatacaaca gatgattcta aaaataatca
    14761 aagctctacc aaaaagcagg aagataataa tatcaagcct actttttcat attcttcaat
    14821 accagataat ataaaaaata aaatgattgg taaatctatg cctactgatg aacctataag
    14881 ctttgatagt ctatcttatt taaaacttac atattatgga tttgatgaaa aaactcatca
    14941 aggagaaatg atagtaaaca gtgaactagc tccagaagta gtggatattt ttaaggaatt
    15001 atacgaaaaa aaatatccaa ttgaaaaaat taaattaata gatgactatg atgctgtaga
    15061 tgaaaaatct atgtctgata ataatacctc atcattttgt tatagaacta tagctggtac
    15121 aaatgtagtt tcaaatcatg gtaaaggacg tgctatagat ataaacccat tacagaatcc
    15181 tcaagtttct ggaaatgatg ttacacctaa aataagtaca gtttatgctg ataggtctag
    15241 cacaaaattt ggaatgataa aaaaaggtga tgattgttat aatgcttttg taagcagagg
    15301 ttggagttgg ggtggctatt ggaaaaaccc agattatcaa cattttgaaa aataatagat
    15361 tagaaataaa ggtgaattat atgaatttaa tgacattgga aaatataagt aaaagctatt
    15421 ctgaaaaaaa attactagaa aatatatctc ttggaataaa tgagggggaa aaaataggac
    15481 ttattggtgt aaatggtact ggtaaatcta ccttactcaa aattattgca ggagttgaag
    15541 aaagtgagac tggtaatata acaaaaacta atggtattag gatagaatat ctacctcaaa
    15601 atccaactta caatgaaaat tcaactgtac ttgaacaagt atttaatgga acttctgaag
    15661 aattaaagct acttggtgaa tatcaagaaa tcctagaaaa acttaattca aactttacag
    15721 aggaattaaa taaaaaatta ttatcagtac acgaaaaaat agatgcctta aatctttggg
    15781 atttagaaag tgaagctaag tcagtactta caaagcttgg tataaatgac tttaatcaaa
    15841 aaatagcaga actttctggt gggcaaagaa agagagtctc tctggcatca gcccttatta
    15901 caccttgtga gtttttgata ctagatgagc ctacaaacca tctagacaat gatactatag
    15961 attggcttga ggaatatctt aattcaagaa aaggctcatt gcttatgata actcatgata
    16021 gatacttttt agaccgtgtt acaaatagaa ttattgaact ggacaagggt agattattca
    16081 gttatgatgg gaattattca atatttttgg aaaagaaaat ggaaagactt tcgctagaag
    16141 caagtattga agataaaaga caaaatttaa taagaacaga gcttgcgtgg gtaaaacgag
    16201 gagctcaggc tcgtagcact aaacaaaaag ctcgtttaca aagatttgat gaacttatga
    16261 atagagattc ttttaaacct gatgaaaatc tcgatatatc tgttgcatca agtagacttg
    16321 gaaataaaat aatagaaata cacaatatat ctaaatcatt tgaagaaaat actgttatag
    16381 ataatttaga atacacttta actcgtacag atagaattgg tataataggc aaaaatggta
    16441 tgggtaaatc tacccttata aatatactta atggagaaat agagcctgat agtggatata
    16501 tttcaattgg agaaacagtt aaaataggtt gtttttctca agacgactct catatgaata
    16561 ttgatatgag ggctatagac tatgtaaaag aagctagtga ctatatagaa acttcagatg
    16621 gtactaggat tacagcttct caaatgtgtg aaagattcct ttttaatggt actatgcaat
    16681 atactttgat aggtaaatta tctggtggtg aaagaagaag actacatctt cttaaaatcc
    16741 ttatgacagc tcctaatgtg cttcttttag atgaacctac aaatgattta gatattgaaa
    16801 cgcttaaaat attagaagaa tacctagacg aatttaaagg tgtagtagtt acagtatcac
    16861 atgatagata tttcttagat agaatttgta ataaaatatt tgcatatgaa ggtaatggaa
    16921 aaattcatat ctatacaggt aattacagtg attatcttat ttatagagag attcaaggaa
    16981 ttgaatttga agaatctaaa agggaatcag ttaaaaaaga tgactctact accctaaaaa
    17041 aagaaaaacc taaaaatgat aaaaagctta agttctcata taatgaacaa agggagtttg
    17101 aaaatattga ctctgatata gaaaaactcg aatctaaaat tgcaacactt gatgaaagta
    17161 ctgcaaaatt ttcaactgat tttactaaac ttcaagaaat tttagatgag aaatctaaac
    17221 tagaaaagga acttgaatac aaatatgaaa gatgggaata tttaaataat ctagctgatg
    17281 aaatagctaa taataagtga tattaaaata atcttaagtt taataagttt tgctttaaat
    17341 ctatacttta taaatatttt aaaggtagtg tctcaaaatg aactttttag ttttatgaga
    17401 cactcttttt tatgagaaat caaatatgtt ttcatcattt caacaataca aaagaggctt
    17461 atctctattt tgagacagcc tctttcataa ttaccttcta atttatatgc tttaatatat
    17521 ctataataat tcttattata tctatatatc cagtctttca atatagtgac aattaacttc
    17581 aatatgattt aaaaatacta acaacctttc ataaagtgga tgagattctt ctccatattt
    17641 agcttctagg ttcttgccta tttctctaac agtcctttga ccatcaattt gtaaaaaaac
    17701 aaaactggaa tattcatcta gttctatttt tttgtattta ggtattctag cttttaactt
    17761 tctaaaaaac ttttgaattt tatggtcctg tttttcaagt atagtaacaa cactattttc
    17821 agctacttca tattcaagtc catcagtaat tttaaataca atatctaaaa catcattact
    17881 cttgctcatt attttttact ttagataaaa caataggtaa tgttgtaagc acaactaaaa
    17941 ctactaataa agcaattgcc attgaatttg ttgcagcaaa tccacttggt acctttggag
    18001 ttacaactcc agttacttgt aatattatac ctattaaacc aatgatagaa ccaccagcaa
    18061 caagaccaga agataaactt ataccattag aaactttaac ttctttttct ttttcagttt
    18121 tagatactaa ttcaacaaat aaacgaatta atgctccaac taatattata gaagttgttg
    18181 caattggtaa atagaatcct atagctatag tcattattgg taaatttaat gcatatagaa
    18241 ctattgccat aaatactcca acaataatca taacccaagg taaactacct gacataatac
    18301 cagaagttaa tgttgacatt aagtttgctt gtggtaatgc aaattgtaca ttatcaccag
    18361 ttgctctaag ttggtcagaa agtaataaaa ttactcctac aacaactata acaccaacta
    18421 tacttgcaat tgtaaagtaa cgttgcattt cgtttttgct accaccaata atataagtta
    18481 ctttttgaga ttgagtataa cctccagcta tagcaatagc aacaactata aatgaaccaa
    18541 ataaaagtaa tgatttatta gcttctaaat cagtccatcc cattataaca aacactaatg
    18601 ttacaataac tagagaagct atagtcatac cagaaactgg aagatttgat gttccaattg
    18661 ttcctgttaa acgaccagct actatgacaa ataataaaga taataaaaga gatatgatag
    18721 ctccaataat tgccataaca atgttaccag aaattaagaa tgctgctaaa aatccaataa
    18781 ctactccacc taataaaagt atcatttgta tagatgaacc ttcttctcct tctccagcac
    18841 ttgatttagc tttaagtgtt tcttttatag aagcgattat agttggtata agttttattg
    18901 caccaataat acctccacaa agcatcatac cagcacctat atattttaca taactactag
    18961 atattgctcc gacatccatt tggttaagag gcattgcagg attattccat accatagctc
    19021 catctttagc catatctgta aagtatccta ttaaaggtgc tataccaaag tttgataaaa
    19081 tacttcctgc aaacattgtt aaagaaactt caagccctac tataaatcca attcctaaaa
    19141 gtagaggatt tacttcaata tcaaatttcc atttgtagaa tttgcttcct acaagactca
    19201 ttacattgtt tgctacattt aagaatgaac cagttaatac agtaataaat ccacttatta
    19261 caaatccaat tcccatgtac ttaattgaat ctccaccagc ctctgaagca acaagtgtct
    19321 cagatatagc cattgattct ggatacataa gtttaccatg ttcttcaact attagatagt
    19381 tgtgaactat agcagcaatt cctataccaa ataaaactcc accaactcca acagctaggc
    19441 cttcaaagaa tgtaatttgg cttccaatta atataactgc tggtaatacg aaaataaatc
    19501 cacttgcaac agattctcca ccacttgaca tcccttggat taagttttta ccaagaatac
    19561 cctttgaacg agcaaatgct ccaacaaaag cagaacctat tatagctcct ggaatacctg
    19621 cagcaactgt aagaccggct ttcattccag agtaagttgt agatgcggca aaaatggcag
    19681 ctaaaataat accgataatc agtacagcta catttccacc ggttctggac ttatctgtga
    19741 tatatggaac gtaatctttt ccactaacct caccataagc acccttaggt aattttttgt
    19801 tcataaaatt ttcacccctt ttcaatttgt atatattata acatatatac atataaaact
    19861 taacttttta ttattttaca aatagcgagt caaaactact aaaatttaac cctatattat
    19921 taatttctac ttttagatat ttttatgcta attttctttt tatttttcaa ttctactaaa
    19981 aactattaag atataaaata aaaaaaattt ttaatgtaaa attaattttg taaaaaaaat
    20041 gctttaaaac acggaaaagt ataaaaaata caataatcat tcttataaat aaattttcac
    20101 catagtaaat atatacagaa aattcttttt tttacatcaa ttgatttcct cagaaaaata
    20161 ggtgtcataa agaaatgtat ggaatcactt tacaacacct gtcataataa aaaatcactc
    20221 tggtagtgct tttttattta cttttcctat tggtgtaact ggtattttat ctatcctcat
    20281 tattattgtt ggaatcatat atggaggtag atgctcattt aaatattctc taatggttct
    20341 ttcatttatc ttaccactat cttcctcatc aattacataa tatgcacata tatacatatc
    20401 cccgtcatca tttgttctcg atatacaaac aacactttta atttctgtaa tattatttat
    20461 tatattttca atctcatata gctctatcct aaactcatca atttttactt gattgtccat
    20521 ccttccaata taatctaaat ttccatcatc taaccatctt actaaatcac cagttctata
    20581 cattaactta ttatattcac aagttttgta aggattctct acaaatactt gctctgttag
    20641 ccctttttta tttaaatatc ctcttgatat ttgaatgcct gatagacata gctctcctgg
    20701 tgctccaatt ggacatagtt tgttcttttt atctaataca tatgcataag tattaaatat
    20761 aggttttcct attggtattt ttccatattc tctgtccaca tgaaaagaag tcgatacaac
    20821 agtaaattcg gttggtccat actcattata tagttcataa ttacgctttc tatatccttt
    20881 taatctttct cctccagtct gtacaactct aagtgaatta ttattgcaat tttgtataaa
    20941 taaccttgca aatgctgttg gcaatgctag tatagttatg tcattttcct cacagtattc
    21001 atttactgta tattcatcaa atttagcttc ttcaggcata atatctattg atgcacctac
    21061 aagcaaataa gggaatagtt gtttcattac agcatcaaaa ctgaaactta agtatatacc
    21121 acatatatca ttttctgtag cgcttataga ttttacgcta taataacaca tatttactaa
    21181 actcttatgt tctatagcta tggtctttgg cctaccagtt gaccctgaag tagatatcgt
    21241 atatgcacaa tttgatgatg ttattttttc agttaattca tatccaagct taaacatttc
    21301 ttcatcattt atatcgagta ctatggtatt ctcatttaca accttacttt tgagttcatc
    21361 tgtagttatc actacttttg cattactatc ttcaagaagg taattcaatc tttcttctgg
    21421 attactcgtg tctattggaa aaaatgctcc ccctgatttc ataatcccta ttgctgtaat
    21481 aattatgtta atatctcttg gtaacattac agctactatg tcttcacttt ccactcctat
    21541 atgatttaaa taacctgcaa gtttatcagt tacctcatcc aattctttat atgttaactt
    21601 tctattttca tccctagcag ctatattact aggtgttacc ttaacttgct ccctaaataa
    21661 atcaacataa cttttatcat agggtacttc atattgatta ttattaaagt cttccaataa
    21721 tactttttct tcttccttac atacaaattc taaatcttct attaaaatat cattatcttc
    21781 tattatttgc tctattatat aatcaaattg atttaaaatt cttctaatag tttctctttt
    21841 atatatagat ggtttatact ttatttttat tttaagtgcc ctcttagtat atactattac
    21901 agataaatca tatccttttt tttctatgtc ataatcatat gataaatcaa aacttttagc
    21961 tataaatatt gtgtttataa gcttagtatt tagatgattt ttattctcta tatctgataa
    22021 caaatagtat ccatactcta aagacctgct ccactgcctt tttagcttcc ttacaacatc
    22081 tataaatttt tcactttgtc taactcttac tcttattgga attatattca ttgttttacc
    22141 aaaaactaca tcatcttcat atatatactt ttgaagtata aggccatata tagtttctat
    22201 gatagagtct atgctaatat tgttcacttt agaatattga attaaccttt catgtttctc
    22261 aatattcatc ttaaaaacct catctactac ttcatcagaa caatctttat aaaaaaaatc
    22321 atttggaatt tttgctatat tcgtataatc atctaacaaa tccatccaat aattcatagc
    22381 tcgttctaaa ttaacttcat acatatcaaa attccccctt actactaagt ctatagaaaa
    22441 aattcttttg gaattttatt ttcacaatat ataaatatat taataataca tatgcaagct
    22501 ctaaaaacat gagcttgcat aaaattacac ttattatgac tactaaattc gtaatattta
    22561 caat
//
LOCUS       DAMJVX010000045        22295 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMJVX010000045 DAMJVX010000000
VERSION     DAMJVX010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22295
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.047"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            64..585
                     /locus_tag="PC407_001623"
     CDS             64..585
                     /locus_tag="PC407_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2497074.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFNEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
     gene            1376..3454
                     /locus_tag="PC407_001624"
     CDS             1376..3454
                     /locus_tag="PC407_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDF2497075.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            3454..4233
                     /locus_tag="PC407_001625"
     CDS             3454..4233
                     /locus_tag="PC407_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2497076.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            4226..5035
                     /locus_tag="PC407_001626"
     CDS             4226..5035
                     /locus_tag="PC407_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2497077.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            5055..5537
                     /locus_tag="PC407_001627"
     CDS             5055..5537
                     /locus_tag="PC407_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2497078.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            5558..5962
                     /locus_tag="PC407_001628"
     CDS             5558..5962
                     /locus_tag="PC407_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2497079.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANDDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(6118..7278)
                     /locus_tag="PC407_001629"
     CDS             complement(6118..7278)
                     /locus_tag="PC407_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2497080.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            7486..9036
                     /gene="xylB"
                     /locus_tag="PC407_001630"
     CDS             7486..9036
                     /gene="xylB"
                     /locus_tag="PC407_001630"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDF2497081.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNIIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYINTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYKIYHRLYKRIYKSLKDDFKLLDE
                     VVNTKINN"
     gene            9132..10469
                     /gene="xylA"
                     /locus_tag="PC407_001631"
     CDS             9132..10469
                     /gene="xylA"
                     /locus_tag="PC407_001631"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDF2497082.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNVTDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            10991..12190
                     /locus_tag="PC407_001632"
     CDS             10991..12190
                     /locus_tag="PC407_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HDF2497083.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            12357..13142
                     /locus_tag="PC407_001633"
     CDS             12357..13142
                     /locus_tag="PC407_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2497084.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            13311..14876
                     /locus_tag="PC407_001634"
     CDS             13311..14876
                     /locus_tag="PC407_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2497085.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFLFTFLIKKFDMKTPGREEDNEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKVVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            14890..16221
                     /locus_tag="PC407_001635"
     CDS             14890..16221
                     /locus_tag="PC407_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2497086.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARILGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMIEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            16235..16723
                     /locus_tag="PC407_001636"
     CDS             16235..16723
                     /locus_tag="PC407_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497087.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            16737..17204
                     /locus_tag="PC407_001637"
     CDS             16737..17204
                     /locus_tag="PC407_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2497088.1"
                     /translation="MFFKRKKEASTINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            17207..18361
                     /locus_tag="PC407_001638"
     CDS             17207..18361
                     /locus_tag="PC407_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2497089.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMILSLFGCLAYGLIVLLNYLQE"
     gene            18679..19431
                     /locus_tag="PC407_001639"
     CDS             18679..19431
                     /locus_tag="PC407_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2497090.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFIAIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPAVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            19432..21339
                     /locus_tag="PC407_001640"
     CDS             19432..21339
                     /locus_tag="PC407_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2497091.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSLIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKHIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEVKTKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMNDLSGIYILSLVICLIIFVIYGAVS
                     VNKFKSVVYKN"
     gene            21491..22165
                     /locus_tag="PC407_001641"
     CDS             21491..22165
                     /locus_tag="PC407_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2497092.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFMDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIDILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYII"
BASE COUNT         8576 a         2338 c         3937 g         7444 t
ORIGIN      
        1 taaataatat aattatcaaa atatgtagta tcattaaaat taagaataac taaggagatt
       61 attatgaaga gaaatagaga gattgtagta gtatgtcatt gtatattaaa ttgtaattct
      121 aaagtagagg gattgtcaga atttaaggga gcacaaaata tagcgataaa tttaataaat
      181 aaagggtatg gaataattca actaccatgc ccagaaatga ttatgtatgg aataaatagg
      241 tggggacatg taaaagaaca gtttgacaat ttgtttttta gagaacaatg taggcaaatg
      301 ttaatacctc atgtaaggca gtttaatgaa tatttaaaaa atgattatga tataaaaagt
      361 atcattgcaa tagatggtag tccaagttgt ggttataata agacatgtag ttctaaacgt
      421 tggtttggtg agctaagtgg ttgcaatgaa ttgatggata aaataaatga tatacaaatg
      481 gtggatggga aaggtatttt tatagaagag ctagaaaagc tacttaaaga atataattta
      541 gagataccta tagttggatt tgacgagctt agagaagata agtaaagata taaatattta
      601 ttgtctcatt atgaaacacc attatatttg gcagaaaaat atgactgatg gacagtacct
      661 gaatcatttt taagtggagt aattagtact tataaagatg tatctactag ctgaaagaaa
      721 atgtagagag gtaataaatg aattgataat atatataaag gagtgtctaa aagtgaactt
      781 tttagtttat gagacactct ttttttatat taaatcaagt atattaaatc aaatatattt
      841 taatcatttt agcaatgaaa aataaattta tctatatttt gagacaacat cttttttagt
      901 tttatttatt tgttgtcgtt tttgacttgg tatacaatta tatattttct taaataattt
      961 cataaattat ttataattat aaaatccatt gttttgaaca atatctaata tattattatc
     1021 tgtatttata aggtcactat acatattttc aagtccaata ttatctacat aataggcaaa
     1081 ttttattcct gtatgtgttt aaaaaaaact agatgtatat aggtatcatc acagatatta
     1141 aaagtataaa taaaagatat taagaacgac tttcaagcaa catattagaa aattttacta
     1201 aaatccggaa agcgattgac aaacaaagtc aaataaagta tattattggt aagttagttt
     1261 aatgtacaaa tgaattatat ataaaaccta atttataaat aacgatacta aacgacatta
     1321 tttaacttaa gctacacaaa gatagaatat tataccattg atttaggagg tagccgtgat
     1381 aaaaatatct gaagactata ttgaaaaaag atttgacaat gaactattgc gaatagaagc
     1441 atggggaaaa aactctttac gtattaggtc gtttgtagac caaaattttg ttgatgaaaa
     1501 ctatgctctt aacgaaaaac ctaagttaaa taaagatgat attacaatta ataaaaatga
     1561 agatggaagt gcaatcatta aaaatggaaa gataaaagct gtcttagacc atagagatag
     1621 aattactttt tataatgata aaaatgaaat attattgaaa gaatatattc gattaagagc
     1681 tgtaaaacat gatgatggtg gagaagatgt agggacaatt gagattacca aagactttaa
     1741 ctctactcta aagttaaaat caagagaata tcgaccaagc tatagtggag aatttgaagt
     1801 aactactaga tttgaatctg agccttctga aaagattttt ggaatgggac aatatcaaca
     1861 taaattttta gatttaaaga atactgtttt ggagttagcc caaagaaatt ctcaaatatc
     1921 agttcctttt tatatttcta gtctaggtta tggactttta tggaataatc cgggaattgg
     1981 aaaagtatct tttgctaaaa atatgacaga atggaagatg ttttctacaa attttataga
     2041 ttattggatt acttgtggag aaagcccaaa agagttaaat aaaaattatt ctcaagttac
     2101 tggaactgtt cctatgatgc ctgaaaacct tttaggacta tggcaaagta aactaagata
     2161 tagaacttca gaagaagttt tagatgttgt gaaggaatat tctaaaagag gaattaagct
     2221 ttcatcaatt gcaattgact attttcattg gcctaagcaa ggtgaatata aatttgactt
     2281 agattattgg aaaaatccta aagaattagt taaaaaattg aaagaagagt attcagtaga
     2341 accaatagta tctgtatggc ctacagttca aagtgatgca gaaaactaca atgattattt
     2401 agaaaatggc tatttagtca atgttaatcg tggtgttaga atgacaatgc aaattcaagg
     2461 aaatacagtc tttgtagata tgacaaatga aaatgcaaga gaatatgttt gggatagaat
     2521 agataaaaat tataaacaac ttgggattga ctattattgg ttagatgtag ctgaacctgg
     2581 ctattctgtg tatgattttg ataactatag atataaaaaa ggaaatgttt taagttgtgg
     2641 taatatttat cctattgatt atttaaaaat gatttatgat ggtttacata atgacataga
     2701 gtctgttgta actcttgtaa gaggagcttg ggcaggagct caaaagtatg gagctttagt
     2761 atggtctggt gacattgatt ctagttttga ggcttttaat aatcaagtaa atacagggtt
     2821 aaatatggga ctggcaggta taccatggtg gactactgat attggaggtt tccatggagg
     2881 aaatcctaaa gacccagaat ttagagaact tatggtaaga tggttccaat atgctacttt
     2941 ttcaccaatt ttgagaatgc atggagatag attgcctcat agtaaaccat tatcaaataa
     3001 aggtggaggc tcaatggtaa ctggagctcc taatgaaata tggtcttatg gtgaagaagt
     3061 tgaagtaatt cttacaaaat tcataaaaat tagagagagc ttaaaaactt atttaaaaaa
     3121 actgatgaaa gaagcgcatg aagatggaac accagttatg agaactttat tttatgaatt
     3181 tcctgaagat gataaaactt gggaggtgga taatacatat atgcttggag atgaaatttt
     3241 ggtagctcca ataatgaatt ataaagatag aagtagaaaa gtatatttac caaaaggaca
     3301 tacatgggaa aatatattta gtggagttag ttatgaaggt gggaaaactt atgaagttga
     3361 atgcccgctg gaagaaattc ctattttcct aaagcaagat tccagttata attttaaaga
     3421 actaaaaaat attttgggag aggtgaaatc ataatggagc cacaattatg gcaattatta
     3481 ttaatcgtac tatatggatt ttttataaac tatgaaaaaa attcaacaat gtttggaaca
     3541 tatcaaccag taacagcagg ttttattact ggtcttatct taggagatat aaatacaggg
     3601 ttatatattg gaggtacatt acagctatta tcattaggta ttagtaactt tggtggagca
     3661 tcaatacctg attatcaaac agcaagtata gtagccacat ttataactat tacgactaag
     3721 caagaagcat ctgtaggaat ctctataggt attccagttg ctttattgat ggtacaatta
     3781 gatgttctta gaaatactat tggtatatgg ctagttcata aagcagaaga tggagctaaa
     3841 aaaggaaatt ataaaaatat aacctatatg caaatgttag gagtactatt aacagcagct
     3901 acaacaggta ttccagttgc tctatctgta atatttggac cttctttaat taataccatt
     3961 ttgaaatata cacctgaatg gttaacaggt ggtttaacag tagctggagg actacttcca
     4021 gcagtaggga taggattatt acttagatat cttcctgcta aggagtattt tagttactta
     4081 gtaattggtt ttgttttagc agtatatatg aaagtaccat tattaggtgt agctttaatt
     4141 ggtggagcaa ttgcacttat aatttacaaa aagaatttgg aaaatcaaga acaacaatat
     4201 actgtagtag gaggtatgga cgaagatgag tgataaggta tcattaaata aatcaaatga
     4261 aaaggtgaaa aaaagtgtct tatggcgctg gttttttact agttctgttt cttcaaatta
     4321 tgagaagatg caggccttgg catactgtta tgcagtacta ccttttttaa aagtagtata
     4381 caaagataat ccagaaaagt tacaaagtgc tgtattaaat catttacaat tttttaacac
     4441 taatccttgg gttgcccctt atattttggg tattaatgta gcaatggagg agttatcaag
     4501 tgaagagact gaagaggctg ttgcttcaat taaaacaggc ttgatgggac cagtagcagg
     4561 tttaggagat agtttatttg tcgttattcc gtggacaatt tttggagcta tagcagctaa
     4621 tatggcgata gatggaagtc catttggaat cttactgtgg attgtagtga gtgtagcatt
     4681 gaaattatta agtataccac tatttaatgc tggctataca tcaggtacta agctaatatc
     4741 ttcaattgaa aaaagtttaa agattttaac tgagagtaca tctattttag gtttaatggt
     4801 tgtaggtgca ttaatacctt cagttgtaaa agctaatgtt gctttgaact ttcaacaagg
     4861 tgattttaca atgaaaggtc aagaaatatt agaccaaatt atgcctgggt tagttcctgc
     4921 aattttagtt gtgattgttt attgggctct taaaaagaat ataaaaccta tatacttaat
     4981 tttaggagta atggttgttt caataatttt atatgcatta ggtattttaa aataaggaaa
     5041 ggatgatatt agatatggaa atgaaaggta ttaaaaatat tcgtatagat gaccgtttaa
     5101 tccatggtca agttgcaact atgtggagta ataaattggg tgttacaaga ttaatggtag
     5161 taaatgatgc agtagctaac aattctgttc aaaaacaagt tttaagaatg gctacacctg
     5221 caggtatagc gtcatctata attacagaag agactgctat aaaaaatatt actgcaggaa
     5281 agtatgaagg acaaaatgta ttattaattg ttaagtctcc tgtggacctt attccatttg
     5341 tagaggcagg atttaaaata cataatataa atgtaggaaa tatgtcaagt agaaaagata
     5401 caacagttct aagacctaat atcagtgtta ctgaagaaga gaaagaagca tttaaaagac
     5461 ttttagatga aggaattgaa attacaacta taatgacgcc tgatgataag aaaacttatc
     5521 ttgtagacat cttatagatt taaagaggtg tattaaagtg aatagtatta ttttgatgag
     5581 tcatggcaat atggctgaag aagtcttaaa ttctgcaaaa atgataatag gggattctat
     5641 agattatcct actgtaaata tgaaacaaga tgatggaata gaaggaacaa tagaaaaatt
     5701 aaaaaaagct ttagaagtgt acaaggaaag ttcagaaata attataatgg cagatttact
     5761 aggtggaact ccatgcaatg ctgctttatt agtagccaat gatgacagtc gaattaaaat
     5821 tatttcaggg ctaaatttag gtatggttat tgaatcagct tttttagata caaaggaatt
     5881 ggcagcaaaa ttagagaaaa taggtaaaga taatatttct atggttgaac aaaatgcaag
     5941 tttattatct gatgatgagt aggaagatta tattaatatt taaaacaaaa tacttgtgtt
     6001 aatacttaat tgatatacaa tttttaaaaa atatagctaa tcttgcataa actatataat
     6061 aaatctaatt ttagaatgta taatttaaag ggttatggct gtatatagtc ataaccctta
     6121 tatttcatta attaaagtta aattttgtat attcaaaaag ctcttgatat ttacggcaga
     6181 gccaccatat agagatgcct cagaccctaa tgatgaaatt tctatattta ttcctttgtt
     6241 aaaagagcta actaacatat cttttgtaat ctgtaatatc tcagggatat cactaattat
     6301 ttggctattt aaatatatta tttcaggagc ataagttgta atagcattat ttatagctat
     6361 tgttaagtaa ctacaaaact catgaataac ttttttggca ttctggttat cttcataata
     6421 tagttgcttt actatatcag aatctatttt aggaatattt tctaaagaag aaagctgttc
     6481 aaataccttt ttttctgaac aatactgttc taaacagcca cgatttccac aaggacatag
     6541 tttaccatta ggcatgatga tagtgtgacc aatttctcca ctcataccat ttctgccgct
     6601 atataattta ttatttatta taatgcctga accaaatcca ctgtgaatac tgagactaag
     6661 tagtgagtta tgtacagttg aaaaagtatt ctcagctaaa gctgttaagt ttgcttcatt
     6721 ttctatatga ataggaaagt catacttttt gctaaggatg ctatataagt caatctcatt
     6781 aagattataa tatggagtaa acaatacttt attttcacaa gtaatcccat gtatagccaa
     6841 agtaagacca attaccttat aaggagtgtc tattttggat atgttataac tatttataat
     6901 ttcatctatc aattgtataa cattgtcttt acttacttgt atatctgtta atttcttaga
     6961 atttataata gttccatcta agtaagatag agaagaaaat atatagtcgt atccaatgtc
     7021 catacttaaa gaaatccctg cacatttatt aaatactaat aatatgggtt ttcttccgcc
     7081 actatgagta ctatttccaa ttcctatttc atgaactaga gattcatcaa taagtttttt
     7141 ggtaatagcg gatatagttg ctttattcaa tcctatagta gaagctatac ttgccctaga
     7201 aataggacca ttttttataa tttgttcaag taccaatctt tcattcattt ctcgaatagt
     7261 gtatttatca gtaaccaatt tgatattcct ccttaaaata atattgtaat actttttaca
     7321 caaaaataaa aggttatttt gcattgacaa agataattaa atattttatt attagttcat
     7381 aagttagttt aatatactaa caaaaataaa gcaagtaaaa tatacctaaa atataaaaaa
     7441 attaggatag gaaaacgata gttatgaagt ggcattcaag gagggatgaa gttggagtat
     7501 gtattaggtg tggatattgg tacatcaggt actaaaacag ttttatttga taaactagga
     7561 aatattataa aaagttgtac atatgaatat cctcttatac aagagaaatc aggttgggca
     7621 gagcaggatg caaatgattg gtggaaagct gtagtcgaat ctataagaga agtagttcaa
     7681 agttcaaata taagcagtga atgtataaaa ggaattggat tatcagggca gatgcatggt
     7741 ctagttatgc tggacaatga aggtaaaaca ttgagaaact ctataatatg gtgtgaccaa
     7801 cgaacagtaa aagagtgtga ggagataact gatttagtag gtgaagaaag actgattgaa
     7861 ataactgcta atccagcgct tacaggattt acagcatcaa agatattatg ggttagaaac
     7921 aatgaaccag atatatatat aaatactaat aaaatacttc ttccaaaaga ttatataaga
     7981 tacaaattga caggtgaata tgctacagag gtatcagatg caagtggtat gcagttgctc
     8041 gatataagaa aaagagattg gagcgatgaa gtactagaaa agttaaatat agataaaaat
     8101 cttttaggta aagtttatga atcacaagag attacaggtt atgtgactag agatgtagca
     8161 agtttgacag ggcttaaaga aggaacaata gtggtaggag gagctggaga ccaagcagca
     8221 ggagctatag gaaatggaat agttaaagat ggagtagttt catctactat aggtacatca
     8281 ggtgtagtat ttgcttatac gaaagagcca aaaattgata aagaaggtag agttcatact
     8341 ttctgtcatg caataccaaa tacatggcat gttatgggag ttactcaagg agctggattg
     8401 tcattaaaat ggtttaaaga taatttctgt caaagtgaag tggaagtttc aaattcttta
     8461 ggtgaagatg tatatgagat tataaataat caagtaagtc aagtaccaac aggatgtaat
     8521 ggattgcttt acttaccata tatgatggga gaaagaactc ctcatcttga cccttatgca
     8581 agaggtgtgt tctttggatt atctccaatc cattctaaaa aagaaatcgc aaggtcaata
     8641 atggaaggtg tttcatatag tcttaaagac tgtatggata taatagagaa tcttaatata
     8701 gacgttaatg aagtaagagc ctcaggtgga ggtggaaaaa gtaaagtatg gagacagatg
     8761 caagcagata tgtttaatca agatgtatat actataaatt caagtgaagg accagcactt
     8821 ggagttgcaa ttcttgcatt agtaggagct ggaatatatg aaaatataca aaaagcttgt
     8881 gatgctataa taaaaacttc aactaaatta gaaccaataa gtgaaaatgt agatacatac
     8941 aagatatatc atagattgta taaaagaata tacaaatctt tgaaagatga ctttaagtta
     9001 ttagatgaag tagttaatac aaaaataaat aattaaatta gcttaaaaag aaaaataaag
     9061 atagaaaata gttttaaatt aagaaaacat acgacatata ttatatgtgt aaaatattag
     9121 gaggacatga aatgagtgaa atatttaaag gaataggaca gataaaattt gaaggagtaa
     9181 aatctgacaa tgaattagca tttagatact acaatcctga acaagtggta ggtaataaga
     9241 ctatgaaaga acacttaaga tttgcaatga gctattggca tactttatgt ggagagggta
     9301 atgacccatt tggagtaggc acagtagaga gaccttggaa taatgtaaca gacccaattg
     9361 agatagcaaa gattaaagtt gatgcaggat ttgaatttat gtctaagatg ggaattgagt
     9421 atttctgttt tcatgacaga gatatagctc cagaaggaag agatttagaa gagacaaata
     9481 agatattaga tgaaatagtt gagtatataa aagttaatat ggaaaagaca ggtataaagt
     9541 tattatgggg aactgccaat atgtttggaa atccaagatt tgttcatggt gcatctacaa
     9601 catgtaatgc agatgtatat gcatatgcag cagctcaggt taaaaaagct atggaaataa
     9661 ctaagtactt aggaggagaa aattttgtat tctggggtgg aagagaaggt tatgaaactt
     9721 tacttaacac taatacagaa cttgaaatgg ataactttgc aagattctta caaatggctg
     9781 tagattatgc taaggaaata ggattcactg gccaattctt aatagagcct aagccaaaag
     9841 agcctactaa acatcaatac gattttgata ctgctactgt tttaggattt ttaagaaagt
     9901 ataatctgga taaatacttc aaaatgaata tagaagcaaa ccatgcaaca cttgcaggac
     9961 atactttcca acatgaatta aatatagcaa gaataaacaa tgttttaggt agtatagatg
    10021 caaatcaagg agacctatta ttaggttggg atacagacca atttccaaca aatatatatg
    10081 atgcaacact tgctatgtat gaagtgttaa agcaaggtgg aatagcacca ggaggattta
    10141 actttgactc taaagtaaga agagcatctt ttgaggtaga agacttattc ctagcttata
    10201 ttgcaggaat ggatacattt gcaaagggat tattaatagc tcataaatta ttagaagatg
    10261 aggtatttga aaactttact aaagaaagat atgcaagttt tagtgaaggt ataggtaagg
    10321 atatagtaga aggtaaggtt ggatttaaag agttagaatc ttatgcactg caaatgccag
    10381 ttataaagaa taagtctgga agacaagaaa tgctagaagc tatacttaac agatatatat
    10441 atgaagtaga tacaatttct aataagtaat tgtgtagtaa ttaaacttgg atgtttgaat
    10501 aaaataacag gatatgttct tgtttttatt ctaaaacgga caaagataag tatttgtaaa
    10561 aattctcata atcatgataa attaaggata tagacttaag tataattagt ttatatcctt
    10621 aaaatttcta taaaaaataa tcatttctat agttatcaat atttttaatt atcaatacct
    10681 ttctcaacta ataaataaaa atcagtaaag tatctaaatt taagtcatta taatggataa
    10741 attttttagc atatcttttt tagtatatca ctgataagaa tttactttca ggatatagca
    10801 gtaaaataga tatttgaata taattgttat tttataactt tcatgaaata attttgaaaa
    10861 tacaaataaa aatttttaaa tagaataata gtggaatata tcttcaagaa tttcttttaa
    10921 ctgtttaggt gataataaaa taatgataca atgtattaaa atataaaatc tgaaagtgga
    10981 tgagaattac atgaaaaaga aagtattatt tttagtaatt attttagcat ttataatctt
    11041 attaggtaat tctacttatt ctcaaaagaa tcagttgtct gaagaggaaa aggtggatga
    11101 ctttaattat gtgtataatg ttgtaaagac aggatatcca tatctaggtg taaataagag
    11161 acttaataat attgattggt tagcaaataa ggatgaatat acaaaacgaa taaaaaacac
    11221 tgaaaatgat gaagaattta tagaagaact gtcatctata ttatctgatt taaataataa
    11281 acacacagaa gtaatagata ataagaaaag atatgaactt tttaagaaat catactctaa
    11341 taatgattgg tatgattttc ttaatgataa aaaagttgtg gatagatatg actctatgaa
    11401 tcttaaaata aaaatgccag atgatatttt tgtaaaaaaa gaattaattt taaaggatgt
    11461 tattaagggt cagattggat atatgtattt accatcaatg tcatcaaaga atggctctat
    11521 aaataatgat ttaaaaataa ttggagatta tattaacact ttagaaaaac ataaagcttt
    11581 aattattgat ataagaggta atttaggtgg aagtgatagg tattggcagg gaatcgtatc
    11641 taaactcata aaaaatgatg tcaaaataaa tggttataga atttatagaa ataataatga
    11701 aatagtaaaa aaatatacaa atgcaagaaa tataaaatta aatcctgtag aaaatttacc
    11761 agcaagtgta aaggaaaatg cacctaaaga aattataaaa gattttaatg attttgaaga
    11821 tacttcctat actataaaat ctacagatga ttcaaaattt aagggtaata tatacttact
    11881 tgttgataga aaaatatatt catcatcaga gtcatttgca atgttttgta aagaaactaa
    11941 atttgcgact ttagttggtg aaactactgg cggagatgga gggtgtatag accctgtatt
    12001 gtttaattta aaaaatagtg gtctaatagt tagaatggca agttgtatgt atttaaataa
    12061 gaatggaata tgtgatgagg aatttaaaac tattccagaa tttaaagtaa aagattgtaa
    12121 aaggactgct gattttaaag atgataattg cataagaaaa gcactagaac ttgaaaatat
    12181 taattattag ataaaagaat aaaattaaaa ctacattttt ttaaacatca attatagcag
    12241 tattttttat agtcaatttt atgggctaaa tagtaaatat aagaatacgc tgtaatttaa
    12301 tgttgaatat tggtataata tttatgacga tttttaatta aaggggataa tagtttatga
    12361 gattagaaga aatgattttt ttaaattata ataaattgaa tgatacagat ttattaatat
    12421 ggaaatatat aactgcaaat aaaaaacatt gtagtaagat gtcaataaat gaacttgcta
    12481 tgaaatgtaa tgtgtctcgt gcaactatta caagatttgt taaaaaatta gatttaaaag
    12541 gtttcagtga gtttaaagta ttattaagct gggaatcaga aaaggttcat gtcatagaaa
    12601 atcagtcatt tgatattgca tgtaattcaa taattaagta tgttgaagat caaaaaaata
    12661 aagattatga tgcattgtgt aaacttcttt atgaatcaag aacaatatac gcttatggtt
    12721 caggtgatat tcaaaatgtg gttgcaaaac agataaagag aatgttctta tcatgtcaag
    12781 aaataatata tgattttgag ggaagaacat ttgatgattc tttttacaat attgtcagta
    12841 aaaatgatgt tatgattatt atatccctaa gtggtgagaa tgagaataca cttaatattg
    12901 ctagaagatt aaaattaata ggaactaaaa taatttcaat tacagagttt aaaaataata
    12961 cattgactga tttaagtgat gaatcaattt atatttcagc gactaatatc tcatttttac
    13021 aaatgcaccc tagttataaa ttgacaatgt tatattttat attgattgag ttattattta
    13081 ttaaatatag tatttataaa aggcatagaa tgattacaga agggattgat tgcaaattgt
    13141 agtcattctt ttttttatga gaatttgaac ataaaaatgt gcaaaaactt atttgactgt
    13201 gaataagctc caaatgtacc ctaaagattg tatatattta ttaacctgtt aaaataatag
    13261 atatctaagg ccaaagataa actaaaaata tgaggagggg agtaatttat atgatgaaga
    13321 aaatacagcg ttttggaggg gcaatgttta caccaacact attatttgct tttgccggga
    13381 taatggttgg attctcaatt gtatttcaaa atcagtcaat tatgggttca cttgctacac
    13441 cagaaaatat ttggtaccaa ttttggggtg taatatctag tggagcttgg atggtattta
    13501 atcaattacc attattattt gctatttctt taccaatagc acttgcaaaa aagcaacaag
    13561 caagagcttg tatggaggct ttagcaacgt atttgacttt taattatttt gtaggttcta
    13621 tgttaagttt ttggggaaaa agctttggag ttgatttcgc agctgaaata agtgctggaa
    13681 gtggacttgt aagcattgct ggtattaaaa cattagatac tggtatggtt ggtgctttgt
    13741 taatatcagg tattgtcatt tatattcata atagatttta tgataaagaa ctaccagatt
    13801 ttattggatt atttagaggc tcatcacttg tagttgcaat ttgtttcttt gttatgatac
    13861 cagttgcatt attaacttgt tttatttggc ctcatattca aaatgttatt agacatttgc
    13921 aaacgttttt tataaatagt ggaaatatag gagtttggtg ttatgcattc ttacaaaaga
    13981 ttttaatacc aactggatta catcattttg tatatgcacc aatttgttat gatagtgttg
    14041 ttgttccagg tggtacatca gtatattggg ctactcatat tcaggacttt caaacttcag
    14101 ctaagactct aaaggaaatg tatccaatcg gattttcatt atctggatta tcgaaggtgt
    14161 ttggttcatt aggtgtattt ggtgctttct atgtaactgc aaagccagaa aaaaagaaaa
    14221 aagtattagg tttaatgatt cctgctactc ttactgcagt attaactgga attactgagc
    14281 cgttagaatt tacattttta tttgtagcac cacttttatt cctagtacat gcatttttag
    14341 atgcatgctt gcaaacgatt tcgtttgcct taggagttgt tggtgatttt ggtggaggaa
    14401 ttattaactg ggttgtatta aattggcttc ctcttggaat gtatcactgg aaggtatatg
    14461 ttgttcaagt tgttgtaggg attatatttt catttatctg gttcttttta tttactttct
    14521 taattaagaa gtttgatatg aaaactcctg gtagagagga agataatgaa gaaacaaaac
    14581 tatatactaa aaatgaatac ttagaaacta aagatgaaaa aggaaataaa ttgagtaaag
    14641 cctctcaaca agcatctgaa tacataaaat tagttggtgg agcagaaaat gttgtagatg
    14701 ttacaaactg tgctacgagg ttgagattaa ccttgaagga tgactcaatt atttctaaag
    14761 aagaagattt taaggtagta ggtgcacatg gtttagtgca taatggaaaa gctattcaga
    14821 ttattattgg gctttcagta ccaagtgtaa gagaagaatt tgaaaattta ttataaagga
    14881 gaaggaaaga tggaaagaaa aagacagagg atactaattg ctggtggggg tagtacttat
    14941 actgctggaa ttgtaacaat gttaattgaa agtgttgcta aatttcctat tgaatcaatt
    15001 aaattatatg ataataatga tgagagacaa agaaaagttg ctgaagcatg tgcaataatt
    15061 gttagagaga aaaatccaga aattaaattt agttatacaa caaatccaga agaagcattt
    15121 actgatattg attttgtaat ggcacaaatt cgtgttggtt tatatgcttt acgtgaacaa
    15181 gatgaaaaaa ttccattaaa atatggggtt gttggtcaag aaacttgtgg ggcaggtgga
    15241 attgcctatg gattaagaac aataggtcca ataatcgaaa tgattgatta tatggaaaaa
    15301 tattctccaa atgcttggat gcttaattat tcaaatccag cagctatagt tgcagaagca
    15361 tgtcgtgtat taagaccaaa ttcaagaatt attaatattt gtgatatgcc agtatgttta
    15421 gaggaaatat ttgctagaat tttaggttta aattctcgta aagactttga tgttagatat
    15481 tatggattga atcatttcgg gtggtggaca agtattaagg acaaagaagg aaatgattta
    15541 atgcctaaat tacaggaata ttgtgctaaa aaaggatatg aggaatttac acctcaaggt
    15601 caacacaaag aatctagttg gttagaaact atgagagcag caaaggactt attagaaata
    15661 gaaccgacta cattacctaa tacttattta aaatattatt taatggctga tgaaactgtt
    15721 gaacatgcta atccaaatta tacaagagca aatgaaatta tggatagaag agaaaaggat
    15781 acattcgaag aatgtgaaag aattataaaa aatggcacag caagggatac atggtttgat
    15841 gcaagtgaac attctcaatt tattgttgaa ttagcttgtg cattagcatt taatactcag
    15901 gaaagatttt tactgattgt tcctaataat ggagctattg aaaattttgc agatgatgca
    15961 atgattgaaa ttccttgcct tgttggaaaa gatatggttg aacctatgtc tataggtaaa
    16021 attccaactt tccaaaaagg tttaatggaa caacaagttg cttctgaaaa attagctgta
    16081 gaagcatgga tagaaaaaag ttatcaaaaa ttatggcagt cgtttactat gagtaaaact
    16141 gttccaagtg caaaagtagc aaaagaaatc ttagatgaaa tgattgtggc taataaggat
    16201 ttctggccaa cttttaaata aggagaacaa aaacatgata acaaataaaa taaaaaatat
    16261 aacagcagta ttaaaagatg aaaatatatt tctttcagtg gttatggttt gttttctaat
    16321 attacctgtt ttatatatag tgtatttaga attgacatca ttatttacaa acatttcatt
    16381 ttcacaactt ttaaacaatg atgttaatgc aagtttaaat ttaatctcta actttagtgc
    16441 agtatacagt gcatatgtta tttacaatat aaaaaagaat aaagagataa aagaaaacat
    16501 gattgctttc tatttagtct tattagggca gatattccaa atttacacaa taactattgt
    16561 tatcatgttt acctatattt ataaatttgt tggaattaaa caaatcataa gcagtattaa
    16621 agaactacaa tggggaaaga gctataaggc tcttttttcc tcaattgcag tatgcttaat
    16681 atcgttattt gtaatgataa ttagaataaa gttaattatg tagaggagtg tctataatgt
    16741 tctttaaaag aaaaaaagaa gcaagtacaa ttaattcata cattgatgga aaaataatta
    16801 atcttggaga aattcctgat aaagtatttt caactaaggt aatgggagat ggatttgcta
    16861 tgatagcaaa ggacaatatt cttaaatcac cgactaaagg agtaataagt tttattgctc
    16921 aaactaacca tgctatagga attaaattgc aaaatgatgt tcaaattctt attcatattg
    16981 gattggatag tgcaacaaca aaaaaagaaa actttaaatt agaagttaaa caaggtcaag
    17041 aagttaatat gggggaaaag ttagtagaaa ttgataataa gttacttgaa aatactttat
    17101 atattccttt agttattgta gagaatccta atacggtaaa atatcatctt tcaaacttaa
    17161 atcaggaagg aaatagtggt attaaagtta tcaattttga ttgattatga ttaataaagc
    17221 gattaaattt aaatttatca tattctattt tttctcatat attatttttg cctttgcttc
    17281 aacaaaattc acacctttct tatcaaaatt aggttactca gcgtttgaaa gaggaattat
    17341 attatcgagc tatgcaataa ccaatatatt atttcaatta ttatttggag tattagctga
    17401 taagtatcaa acaatgaaaa aaattgtttt aattagttta tcagtttatg gaattgcatc
    17461 agttattatg ttttctactg aatctgcaac ttttataata tacttattac ttgttgcttt
    17521 atcaggagga ttattaaata cttgctgtag tttatatgat acatggataa taggttgtgg
    17581 agataatatt aataattatt tgtcatttat taagactttt gggtcgattg gatgggctat
    17641 tggaagtatg gtagcatctt acttgattct tagattctca tattttggat tatctgtagc
    17701 gattggttta attgtagtat tacttattat aaatatacta ttattacctg atattgaaaa
    17761 gattaataag aaaataaatg taactaataa agatattatt gaattattaa aagataaaaa
    17821 atatatctta ctagtatgta ttttattctt attatactca atggtggtag caaataactg
    17881 tactgtaata gataaaatga tagctttaaa tgctacaaat tctcagatat ctttaaagtg
    17941 gtctttacag tcattattag agataccaac atatctggct ggttcttatt tattaaaaaa
    18001 atatagcagt ttaaacttat taaaattaag tgctattatg ataacagttc agtttctatt
    18061 atttgcttta acaaatgatt caaatatcat tattatactg tgtgtttttc aagtgttttc
    18121 tactccttta attttaatta cttcaaaaag attaatattt gaagtttctc ctaaaaaact
    18181 tagaagtagt agccaattaa tagctttaag tatatttatg ggaggttcat ctctgattat
    18241 tccaactgtt gctggttttt tgagtattaa tattgggtat aattatacat taatgatatt
    18301 aagtcttttt ggatgcttag cttatggctt gatagtctta ttgaattatc ttcaagaata
    18361 attgttttaa tatatagtag taatatcact tgtttatagt acctgtattt gccacaatca
    18421 aggttgtatt tttggttttt tatagagaag aattattgta tagattagag gcttcttagg
    18481 ggcttctttt ctttgtttat acaaaaaagt gaatcatgct tttattttaa gatatttcca
    18541 ctataattat aatagtcttc catatgttac aaaagtgtaa tgctaacagt taaaatgtaa
    18601 tatttactta aggatttatt tatataaaac ctttaaaata aatatatatt aaaaaattag
    18661 caactggagg ctattattat gaaagaaata ttaaaaataa aaaatatatc aaaagattat
    18721 ggaataaaag gatttaaaac aaatgtgtta aaaaatatat ctcttacagt caatgaaggt
    18781 gattttatag caataatggg tccatctggt gcaggaaaga caacattgct aaatttaatg
    18841 tctacacttg acaaacaaac ttcaggagaa ataatactag atggaattaa tatatcaaaa
    18901 gtaaaaaata atgaactatc aaaattaaga agagaaaaaa tagggtttat atttcaagac
    18961 tataatctat tagacaatat gaaactaatg gacaatatag cacttcctct agcattagga
    19021 aaaaagaaaa gcaaggagat agaagctaag gtattttcta tagctaaaaa atttggatta
    19081 gaaaatcatt tagataaata tccatatcag ttatcaggag gacaaaaaca aagaggcgca
    19141 gcagctaggt cattaattac aaatccagca gtcatatttg ctgatgaacc aacaggtgct
    19201 ttggactcta aatcagcata tgaactttta gaatcattag aaaaaataaa cagagaaaac
    19261 aacgctacta ttataatgat tacacatgac cctctaacgg caagttattc aaatgaagta
    19321 tatatgataa atgatggaaa tataaagtgt aaattaaaca aaggaaatag tagaaaagaa
    19381 ttttatggaa aaattatgga tatgctagcc tctatgggag gtgagatgta agtgagtata
    19441 cttggaattg cttataataa ttttaagagt aacataagaa cttatctagc attttttata
    19501 tctatggttt tttcagtagt tgtgctgact aattttgaat tattaagata tggagatgct
    19561 ataaatgtgt tacaagagga aaataaaaaa tttacactat cagtgttagt agctgtaata
    19621 atgatactat cagtattttt atttttcttt atatggtatg caacaaatat attttttaag
    19681 aataggtcta aagaaattgg aatattgtcc tttatgggat tagatttata tactatagga
    19741 aaaatatatt ttgtagaaaa tatgcttata ggaataagtt cttgtataac aggaataatt
    19801 atagggataa taacatctag attttttcaa gtagtgataa taaaattatc tggatttaat
    19861 ataaaggtat ctaatggact tagcatagaa gctatactat atgctacttt aatctttatt
    19921 tctatattta taataatggc tgtaaaaggt tttataacaa tatgtagaag tagtgtaata
    19981 aatctaatca atatatctaa aaaacaagaa aaaataccaa aggtaggaat aggtatatat
    20041 atattagcta tagtatcact tatagtaata ggatatggat actatctttc tacgaatata
    20101 agaactggag atataactag tgtactacct gttataggta taataatagt aggtacttat
    20161 ggcttattca aatcagttat gcctgttata tttgatatta taatcaaaaa taaaaaaata
    20221 ttatttaatg gaaataatat aattgcgata aacaatataa actaccgatt aaataaaaac
    20281 tataaaactt atgctataat agctataatt ataactacaa caatatcaac tcttggtgct
    20341 tcagttgcta tgaagcatat acataagaat gcccaagaac aaagaaatgt gtacactatt
    20401 agcatagttt caatggataa agatgaagtg aataaaaaac aaatagagaa cataataaaa
    20461 gatactaata agataaagta ctccgtaaat ccagaactaa caatcttaaa gaaaacagat
    20521 ataaaagaag cttatgatac aacagatact ataatgaaat atagtgattt ttgtaatact
    20581 cttaaagtga atggcaatga taaagaatta aaagagtatg gtgaaagatt agttagtgga
    20641 aataatgtaa tagagctaaa aagtcctcaa acgatagcat caatagctac aaaagaagtc
    20701 aagacaaaac ttgatggtca gttatataat atttcaaggg gagaagtaaa agttccagta
    20761 ttagggacag gacttaatca aagtattata gtggtaaatg atgatacata ttttaaacta
    20821 agagataagg gtcaacaagt atatttttat ggtgcaaagg tatttcaaga agaaaatagc
    20881 caagtaatgt tcaacaatat tgaaaagaat ttaagtttaa aaaatggata tattcagaat
    20941 ggatataaag ctcaaggtga gagtcaatgg atgaagtttg catatgtagt gcttgtgttt
    21001 ttattcttag tctttgcaat tgtagctgga agtataatat atatgaaaat atatagtgat
    21061 gcatatgaag ataaagagaa atacaagatt ttattaaaaa taggtactac agaaaaagat
    21121 ataaatagag ctatacttaa agaagtagct atattttata ctttgccaat gttaagtgca
    21181 actatatcta gttattttgc attgagatta gcaggagatt tattgatgaa cgatttatct
    21241 ggtatatata tactaagttt agtgatatgt ttgataatat tcgtaatata tggagcagta
    21301 tctgttaata agtttaagag tgtagtgtat aaaaattagt tgatatatta ataaagagaa
    21361 aggagattct tgtaataaga gtctccttgt tgattaacta aataatttgg gtaataaatt
    21421 tatataaaat atgttatgat attaataatt catggcatag tgaaatatta ggataatgga
    21481 gaatatacaa atgtatagaa ttttattagt agaggacgat attgatttat caaaagagat
    21541 tgcgttagca cttgaaaaat ggggatttaa agtagaatta attgatgatt ttgaggtagt
    21601 attagatgaa tttatggata aaaaaccaga tgtagtactt ttagatgtaa atttaccatt
    21661 atataatgga ttttactggt gcgaaaaaat aagagctatt tcaaatgtac cattaatatt
    21721 tttatcatca agagattctg atatggattt aataatgggt ataaataatg gagcagatga
    21781 ttatattacc aaaccatttt ctatagacat attggttacc aaaataaatg gtataataag
    21841 aagagtctat aattatagtg attctaatag tattttgtat tgtgaagatt tgatgtttga
    21901 tgtggggaaa gggattataa aacataagta taaggataaa agtatagagt tgactaaaaa
    21961 cgaaataaaa attttaacat tattattaaa aaataagaat agggttgtat caagggaaag
    22021 tcttatgatg actctatggg ataatgatga gtttgtgaca gacaatgcac ttactgtaaa
    22081 tatgaataga ttgagaagta aagttaaaga actaggtttt gatgatttta taaaaactaa
    22141 gaaggggata ggatatataa tataatgcta gtaaaatatt taaaaggtag gtctaagtat
    22201 attgctctac ctttagttgt agttagtata ataaatatct atttatttgc tatagatatt
    22261 ttaaaaaata gatattcaga attaatatac ttaga
//
LOCUS       DAMJVX010000046        22156 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMJVX010000046 DAMJVX010000000
VERSION     DAMJVX010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22156
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.048"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(145..522)
                     /locus_tag="PC407_001642"
     CDS             complement(145..522)
                     /locus_tag="PC407_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDF2497093.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            complement(638..1132)
                     /locus_tag="PC407_001643"
     CDS             complement(638..1132)
                     /locus_tag="PC407_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2497094.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSVYIGDEKFNNLKNECIKK
                     GIDIKISKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKNIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            complement(1281..5534)
                     /locus_tag="PC407_001644"
     CDS             complement(1281..5534)
                     /locus_tag="PC407_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2497095.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKISYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            complement(5915..7084)
                     /locus_tag="PC407_001645"
     CDS             complement(5915..7084)
                     /locus_tag="PC407_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497096.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            complement(7479..8495)
                     /gene="gap"
                     /locus_tag="PC407_001646"
     CDS             complement(7479..8495)
                     /gene="gap"
                     /locus_tag="PC407_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2497097.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYSDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNQKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            complement(9132..9596)
                     /locus_tag="PC407_001647"
     CDS             complement(9132..9596)
                     /locus_tag="PC407_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HDF2497098.1"
                     /translation="MKKLVIILISIISVVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFAISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            complement(9920..10528)
                     /locus_tag="PC407_001648"
     CDS             complement(9920..10528)
                     /locus_tag="PC407_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2497099.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            complement(10610..11311)
                     /locus_tag="PC407_001649"
     CDS             complement(10610..11311)
                     /locus_tag="PC407_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497100.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            11538..11804
                     /locus_tag="PC407_001650"
     CDS             11538..11804
                     /locus_tag="PC407_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDF2497101.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSMIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            complement(11907..12326)
                     /locus_tag="PC407_001651"
     CDS             complement(11907..12326)
                     /locus_tag="PC407_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HDF2497102.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERIFNLKQEKRKAKHKGH"
     gene            complement(12593..13375)
                     /locus_tag="PC407_001652"
                     /pseudo
     CDS             complement(12593..13375)
                     /locus_tag="PC407_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(13440..14201)
                     /locus_tag="PC407_001653"
     CDS             complement(13440..14201)
                     /locus_tag="PC407_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2497103.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNKKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            complement(15250..16062)
                     /locus_tag="PC407_001654"
     CDS             complement(15250..16062)
                     /locus_tag="PC407_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2497104.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPVNYPNLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNDEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            complement(16432..17334)
                     /locus_tag="PC407_001655"
     CDS             complement(16432..17334)
                     /locus_tag="PC407_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2497105.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDGQISFFD"
     gene            complement(17384..17845)
                     /locus_tag="PC407_001656"
     CDS             complement(17384..17845)
                     /locus_tag="PC407_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDF2497106.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(17897..18352)
                     /gene="def"
                     /locus_tag="PC407_001657"
     CDS             complement(17897..18352)
                     /gene="def"
                     /locus_tag="PC407_001657"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2497107.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(18324..18587)
                     /locus_tag="PC407_001658"
     CDS             complement(18324..18587)
                     /locus_tag="PC407_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2497108.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(18684..19400)
                     /locus_tag="PC407_001659"
     CDS             complement(18684..19400)
                     /locus_tag="PC407_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2497109.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGTLAFIITMILLIFFSLTKISFLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYIYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(19397..20074)
                     /locus_tag="PC407_001660"
     CDS             complement(19397..20074)
                     /locus_tag="PC407_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2497110.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILISIIIIIYFITYYFICSSWK
                     KVGGVKL"
     gene            complement(20071..20916)
                     /locus_tag="PC407_001661"
     CDS             complement(20071..20916)
                     /locus_tag="PC407_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2497111.1"
                     /translation="MIKVDDLSFSYTDRNFLQNINFEVEKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIKKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(21030..21620)
                     /locus_tag="PC407_001662"
     CDS             complement(21030..21620)
                     /locus_tag="PC407_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2497112.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRVVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            21909..22142
                     /locus_tag="PC407_001663"
     CDS             21909..22142
                     /locus_tag="PC407_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF2497113.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
BASE COUNT         7302 a         3771 c         2147 g         8936 t
ORIGIN      
        1 atataaataa aaactatatt aaataaaaat cctaaaatta tttgctgttt atttacaaat
       61 aataattcta aaatatattt gatttattta gtatcgacta taaaaataag ctatcaaata
      121 atttatactt gatagcttat tttcttataa ttcttttttt aaagtttcct ttagactatc
      181 atatgcgaaa tccattattt ctctacaagc aactaaatct ttttttaggt ctatacataa
      241 ctctgaacca tactcttttc ttactgttgt agttaaatct ttagcatata ctttagcttt
      301 agtactttca tcttcgtggt ttctgccttt tacgtatcca attattatat ttgatgcatt
      361 tactgcacca cataaactag caaccccaca ccctgaaccc agtccacttc ctaaacaaac
      421 tggtatatct gtattaaatt cctcattata tgcttttata actgattctg cacaattaaa
      481 tcccttctca tgatattttg atgctataac tttatcacac attaaaattc cccctcattt
      541 ttgtaaatta aaataaattc ttcattgttt ttgtaaaaaa tacttctttt tactattatt
      601 atatcaaata ttaacaataa aaacatttct ttctaagtta actctgtctt tctaaattta
      661 atagtatctc tttcaaatca gaatgattcc ccatatatcc accaatattt tttttgccaa
      721 ctactctatg acaaggtatt attattggaa ttttattact tttacttgct tgtcctattg
      781 ctcttactga ttttggattg tttatatttc ttgctatatc tgaataacac aatgtttctc
      841 cataaggtat ttttaacatt tccctataaa ctgatttttg aaattcagtc ccttcaatac
      901 gaatatttaa attaaagact tttctttttc cattaaagta atcatctatc tggcttattg
      961 cttcctttaa atcttcctta cttatcttta tatcaattcc ttttttaata cattcatttt
     1021 ttaaattatt aaatttttca tctcctatgt atacactaac tataaaacca tcttcactta
     1081 atatgtacaa atttcctaat tttgatttat aaacatcata tactaaactc atattacttc
     1141 ccccctttaa tatcagtata catataattt tttaactaat aaagagttac cctaaaaaat
     1201 aggataactc ttttttatag atacttaata tagagtatat ttttaaattc attaactttt
     1261 aacatatcaa aatataaatc ttatatattt ttaaaagcct tagaaagcat aagttttatc
     1321 ctgttaagtt gatttacctc acttgcagaa gggtcataat caataggtat tatatttgca
     1381 tttttataca acctctttaa ttcttttata gaacccttac caattatgtg attaggtaaa
     1441 catccaaaag gttgcataca aataatgttt tctacacctt catttaataa ttcaaccatc
     1501 tcacctgtta aaagccaacc ttctccagtt tgattaccta atgacactac tggttctgta
     1561 cttttagcaa cattttttat attggctgga gcataaaatc tctcactctt atcaagtgct
     1621 tttttatata ctctttgata tattccagtt atgtatataa atgcttctcc tatcattcta
     1681 ctctttctac ttccttctaa atgagtgtgt ttgtatatag tattaaaggc acaacttaag
     1741 aagaaatttg taaggtctgg cacgactgct tcagcacctt ctcgctctaa tatatctact
     1801 aaattattat ttgcaattgg atggaatttc accaatattt ctccaaccaa acctactttt
     1861 ggttttttga tatctagtat ctctaaatta tcaaattctt ttactatatc ttttatattt
     1921 ctagtaaata cagaaatttt tgctttaaca agagaagatt tgcatatatc aacccatttt
     1981 tcatatagag catttgcact tccagtaact ttttcatatg gtcttactct atatagtact
     2041 ttcatcagca aatcaccata aacaacagac ataaagagtc tattgattaa ttttagtgat
     2101 atattatcca ttataccact atcttctatt ccatttacac taagtgcaat gacaggaaca
     2161 tctttaaatc cagcatcata tatagccttt cttaagaatc caatataatt tgtagctcta
     2221 caacctccac cagtctgagt tatagtaaca gaagtattat ttaaatcata tttgccagat
     2281 tttaatgctg ttattagctg acctacaact attatagctg gataacatgc atcattattt
     2341 acatatctta acccttcttc aattacacta ctatcagtat cttttagaat ttctatatta
     2401 taaccactta agttaacagc tttttcaagg aattggaaat gtattggaga catttgtggt
     2461 gctaatatag tatgtttgtt cgttatctga ttatttttag catactgtat cctgtttacc
     2521 tttatatttt ttacatctat atttttgctt tctctctcaa acatagctgc ttttaaggac
     2581 ctcatcctta tcttagctgc tcctaaattg ttgccttcgt caatctttat aatagtatat
     2641 atcttcgatt tttcattaag tatttcttga acctggtctg tagttactgc atctaatcca
     2701 cagccaaatg aattaagttg aacaagttcc aaatagtttt tatctcttac aaaactagct
     2761 gctttataca atcttgaatg atatacccat tggtctacaa ctctaagtgg tctttgtaca
     2821 ttggctaaat gacatataga gtcttcagta agcactgcca tatctaatga atttataagc
     2881 tctggcattc catgatttat ttctgggtct atatgatatg gtcttccaca taacacaatc
     2941 cccttcatat ttctttcttt tatttcttta agtgctcttt cacctgctgc ttgtatctcc
     3001 attttaaata aattctgttc atcagtagct ttatcaactg catgattaac ttcttcttta
     3061 gttattttta tatcagaaaa atgtcttgat aattcatcat atagtctttt cttcaatttt
     3121 tccttatcat ttagacttat aaatggattt atataattta tgttcttatt tcttatattc
     3181 tctacattgt tttttattac ttcagaatat gatattacaa caggacaatt ataataatta
     3241 tctgcatttg aatcctcttt attttcattt gttacacttg gataaaatat attttttata
     3301 ccttttgcaa ttaaattttc tatatgtcca tgaactaatt ttcctggata acaaactgtt
     3361 tcagatgcaa tactagttat accagtttca tataactgtt ttgatgacct atctgaaagt
     3421 acaactctaa agcccatagc attgaaaaaa gtaaaccaaa atggataatc ttcatacata
     3481 tttaatactc ttggtatccc tatttcccca ttttttgctt cttctttttc caatggtttg
     3541 tatctaaaaa ttctattgta tttgtactta aataaattta ttggtttttt cttttctaat
     3601 cctatttttt tatcaccata aatagcttca cccttctcac atctattacc tgaaataaaa
     3661 atttcattat cagaaaattt atttattgta agaagacaat gatttgaaca acctttacat
     3721 ctagttacac ttgcttctag cttgatatta ttcaactctt ccttagataa caaggatgtt
     3781 ttgtatccac tttcatattt ttctttagct ataagtgctg aaccaaaagc tcccataatt
     3841 cctgaaatat taggtctaat tacatttttt ccaattagtt tttcaaagct tctaagcacc
     3901 aaatcattat aaaaggttcc accttgtaca acaatattat taccaatctc atttaaatct
     3961 ctaatcttta ttactttaaa taaagcattt tttattactg aataagatag ccctgctgat
     4021 atatctccaa cacttgcacc ttctttttga gcttgtttta ctctagaatt cataaataca
     4081 gtacatcttg aacctaaatc tacaggattt tgagaataaa taccttcatg tgcaaattct
     4141 tctattgtca tagataaaga acttgcaaat gtctctaaaa atgagccaca acctgatgaa
     4201 caagcctcat ttaatattat attatcaatt actccatctt ttatttttag gcacttcata
     4261 tcttgaccac caatatcaag tataaaatct acatctttat tgaaaaattt agcagctttg
     4321 taatgtgcta tagtctctat ctctccgtta tcgattttaa gagctttttt aataagacct
     4381 tctccatatc cagttacagt tgaactcaat attttaatat tttttggtaa tatatcatat
     4441 acttcattta taactttaat ggttgttttt aatggatttc cttcattgct attataataa
     4501 gaatatacaa gtcttccatc ctcatcaata agtgctgctt tagttgtagt tgagcctgca
     4561 tcaattccta aataacaatt gctttttatt gaatttatgt ccacataact tattttttct
     4621 ttttcatgtc ttcttgaaaa ttcttcatac tcatttttat ctttaaataa aggttctaat
     4681 aaatttactt ctccatcttc aatattcttt atactatcca atttttctat tagagatttt
     4741 agttgtatat tatcttcatc tactgataat aatcctgctc caatagcaat ataaagttgt
     4801 gcattttctg ggaagattat gttttcatct tttaaatcca atactctttt aaatgcttct
     4861 ctaagttcag ataaaaagta caatggacca cccaaaaacg caacatttcc ttctattttt
     4921 cttccacaag aaaggacact cacagtttgt acgacaactg cattgaaaat actcattgct
     4981 atatcagtct gattggctcc atcatttata agaggttgaa tatctgtttt agcaaaaaca
     5041 ccacatcttg aagctatagg atatataaca ttataccctt tagcaagttc atttaatcca
     5101 ctggcatctg tctttaaaag tgatgccatt tggtctatga atgctccagt gccacctgca
     5161 catattccat tcattctttg gtctattcct ccacttagat atgttatctt tgcatcttca
     5221 cccccaagtt ctatgacaac atcagtttca ggatgaaaat actctattga ttttgtacta
     5281 gatataactt cctgaacaaa ttttacacct aatttttttg atattccaat tccacctgaa
     5341 ccagttatta taatggtagt atttatatct cccaattttt catatatacc atttagtact
     5401 tctttaactg atttttttac atcagaataa tgtcttctat attctttatg aactatatta
     5461 ttactattat taagtaccac aaccttcacc gttgtagaac ctacatcaat tcctatatga
     5521 aataattcat ccattgtacc cctccatatt tatatgcttt tttaattttt gtaagttcta
     5581 tggctttatt ttaagtaagt tatttataat ctttatgact aatattttta ttttatgatt
     5641 cttttattaa tcaaagctta ctctactagg taattcctaa ttctaacctt aaattttaaa
     5701 taagaacata tagttagtgt actaaatttt gtcataaatt tctacagatt tttgttactt
     5761 tttacataaa tataacttaa gtctaatatc cacttaatgt ttatgtatta taaattatct
     5821 ttagtcagaa aaaaatggtt gttgatatgt aaattcttat tacatatcaa caaccatttt
     5881 tttgtatatt attttttatc ttagctgatt acgtttatcc tacgagtgta aatataaagt
     5941 gagctgctgc tcctgcacat aaaccaccta ttgtatgaga aagaatagct ttacctgcca
     6001 actgtctagc atttaatgca tccatcatac caatatgtgt acttaaatat ccactccaac
     6061 acatacccat agctgtaaat actgctatat cattaggagt tatagctcct gactttataa
     6121 actctggaac tagagacatt gctgcaccaa cagctcccaa tgaagttata gggaatgcta
     6181 ttgcctctgg tgatgtaaat ccaaataatg gttctaatat aaatccaagt ttatctccaa
     6241 taactggaag taattttatt ccttcatatg cagctcctgt ataaacttct tgacctgtaa
     6301 cagggtctgt agatggtcca aaagttaata acatcaccaa tgtacatact actaggactc
     6361 caggaattat agccattccc atatctaccc ccattttacc accttcaaga attgcatcta
     6421 aagctctttg aaatacattt ccttctctga tttctctaaa ttcatccttt ttctctaatg
     6481 ttcctgttgc tttatcttct tccggattgt atttataaaa cttcttagta aatgtcaaca
     6541 ttattctaac acttatgacg ctacctataa ttgcacctaa attaccaatt aatgctggaa
     6601 gtacatattc tgtaccttga gaaatcataa atgttgttaa gataagtccc ataccaaagg
     6661 ctgttcctaa gttacataat gctggaactt ggtattgttt aaaatactgt gtgaatgttt
     6721 tatctttagc aaaaggtata atagctggat tatctgataa ataagttgtt atagctcctg
     6781 ctatactagc accaggtagt ccatataaag gcttcataaa tacagcgaaa attttattaa
     6841 ttaacgctat tactccaaac tctgaaagta gtgcgcttaa tgcgcctgca agaactgcca
     6901 tagccataat taagaaaact gtctccaata gtaaagcatg tgctgtactc attattactt
     6961 tgaacatcat tcctgctccc ataatactac caacataccc aaaaccaact actagtaata
     7021 ttaggaaaat gaaggtttcc atgcttactg ccttaacctg tctcctgtct gttaacttct
     7081 ccattttgaa tcatcccttc tttttttgtt ataaagttta acaaaacctt ttctcttttt
     7141 taattatact tataagtatt actatttgca actttatttg tcattttttt atcattttta
     7201 aaatgaaaat cagtatttta ataataaaaa tttttcagat ttatgcaagt tttcgacatc
     7261 ttatctttca ttttataaaa agtatgattc cttactaaat atatatatac ttctctaacg
     7321 acctaatatg caaatataag ttgtaacttg tatctttttg catgaaagaa aaaagattat
     7381 gaaaaaattt ttccttcaat atttttcata atcttttttt ttagttttat ataatttttt
     7441 caaaaaattt ttttaaaatt tgctttaaga aatatatatt attttaattt ttcagctaca
     7501 tattttacta agtcaacagt tcttgaagaa tatccccatt cattatcata ccaagaaact
     7561 accttaacca tattgtcttc tataaccatt gtggatagag cgtctataat agatgatctt
     7621 gaatctcctt tataatctat agaaacaagt ggcttgtcac aatatcctaa aacacctttt
     7681 aattctcctt cagcagcatc ttttaacaca gagttaactt gctcagatgt aacgttttct
     7741 ttaagttcgc acactaaatc aacaagtgaa actgttggtg ttggtactct aagtgaaaaa
     7801 ccatttaatt ttccttctaa ttgtggtagt actcttgaca cagcttttgc tgctcctgta
     7861 gtggtaggta ttatagattc agccgctgct cttgctcttc tcaaatcttt atggctttta
     7921 tctaatattc tttgatcgtt agtatatgaa tgtactgtag tcattaaccc ttttactata
     7981 ccaaattttt cgtccaatac tttagcaaaa ggagctagac aatttgtagt acaagatgca
     8041 tttgaaatta tattgtgctt ttgattatca tattctttct cattaactcc gataactata
     8101 gttatatctt cattttttcc tggagctgtt ataattactt tttttgctcc tgctttaata
     8161 tgtttttctg ccttttctct ttgtgtaaat aatcctgttg attctattac tatatcaacc
     8221 cccagttctt tccatggtaa ttcttctggg tcactatatc ttaatatttt tatctcttta
     8281 gagtttacta ctagagtgtc ttcatccttt gcctctactt cacctctaaa agttccatag
     8341 catgaatcat atgtaaataa atgtgccaaa gattctgttg ttgctcttgc atttatggct
     8401 acaatttcta tattatctcc taattcttct tgagctattc ttaatacagc tcttcctatt
     8461 cttccaaatc cgttcattcc taccttaatt ttcattatga aatgtctccc tccattacta
     8521 tgtttttaca tgattattgt acactaaata aatcatttgt tcaatacttt ttcttgatta
     8581 aataatcaaa ttccatgttt ttttattatc aaaagacaat tagtatatca tttttcttta
     8641 tttcacccct catttagtat gtacttttta gtgtaaataa gctgaaatca tttattttac
     8701 tcaatgtccc ttgggacata ttttgccatc ttttttttga atttttaaac tatacttatt
     8761 tttaaaaata atatatcttc aagaatctcc tctaatttaa aataataaaa acaaattttt
     8821 atttagttca aaaaatatat gattttttaa aatttgccac aattattatt tatatatcaa
     8881 aatataaata ataattagct tattgttaac taaattatgg aaatatactt aattgaaaac
     8941 ttaaatttat ttccttgtgt tatcaagatt tcatgcttac catttccaat atcatgctca
     9001 ccaacttttg atgtgcataa actatttcaa gtaaaaatgc acaacctatc ctttttgata
     9061 ggaaaaagca gtcaatcata aatatcaact gcttttatgt actgaatatc aagttataga
     9121 caagctggaa tttattttga gggtttttct ttctccttaa ctaaattgct ttcaaaagta
     9181 acctttacaa ctgcatgatt tattttaatt ccggtaaagc atatagcata ctctttctct
     9241 ttttgtatat tagaaagaga aattgcgaat gtatcattat caacgcttcc atgccatgta
     9301 ttactccata aagtttcatc tgttttattg tcctttattt ccacaatacc actatctcca
     9361 tccattttaa aagaaatctc tgtgtctaaa acatctatat cattagacac acaaaacaaa
     9421 cgtgcattaa caaatgggtc tgaagtatca taatttttat ctaagtccat ttcaatggtt
     9481 gtttgtcctg attctttgtt gtttaaatta ttatatttac tttcattgct ttttttacaa
     9541 gctgtcaagc taaccacact aatgatacta attaaaataa taactaattt tttcatagaa
     9601 tttatctcct ttacaatttc taatttttaa ccctacaaac tataatatta aactaatcta
     9661 ctattttaca tcaaattttt caatcattca gcctttacct tttctcattt taattgcaaa
     9721 tatgctaaat aattttggaa ttttaattat tgtagtatct taaattggtt tataacatat
     9781 tatgaaaatt taagagtcaa gtaacttatt taaaaataca caaaattata ctttattgta
     9841 taaaaaaaga gattataatt tatacaagta aaataaactt tattatttta ctcttctata
     9901 taacctcttt tctatatatc tattcaaata atttagctcc taaactcaca aacatttgta
     9961 aaatcaaaaa tgcttttgtg tctatcttat gattactttg taaaatctct tttgcatctt
    10021 cttgcgaaat taaaagcgct tcaatctctt catctggttc taaaaattca tctgttatat
    10081 caccatcaca tatacaataa acaaaggcaa cagattcatc actcattcca ggagataaat
    10141 aaactttatc aatactttta ttttttttaa tttctaaaag agtaagtcct gtttcttctc
    10201 taagttctct ttcaacagaa atatctatag actctccttc atcaacaaga cctgctggta
    10261 actcgtatat atatccatta ataggtactc taaattgtct tattaaaatt aattttttac
    10321 tacttttatg aagcccaact attacaactg catctacact atcttcttca ttcttcaaat
    10381 aaatattttt taattgctca ctactttttc ttgatgctac catccaaacc ttatcttcat
    10441 ctaatttgtt tttatactct atttcaaaaa gtcctataaa cttattttca atcattggat
    10501 ttacattctt tattatagtt tttttcaaat ttactacgac ctttcttttt taataccttt
    10561 ttaacatcat taagtaaata taatatctta acaagtattc aacaacttat tatatttcca
    10621 tttcaggatt atctttatct acaaaaccta ctttgatttc acaattaaat atttccttta
    10681 acaattttgc taattcttct cttgcactca ttaccttatc taaattagca tcagtaaaac
    10741 catctatagg aaaaaatata ttttttgagc tattagtaat ttttccttcc tctccttgtc
    10801 tccatatcca tcttctagtt tttaccatgt ctccaactga ataaattaat tctccttctt
    10861 ccattaattc aacatcagtt tctccaaatg gtacaaattt atcccctttt ttagaaaatc
    10921 taacatatac atcatcattt ctaaaatcca tatcatgagc acccattgga agtaaatatt
    10981 ttaaagaaat agagtttcct aaatctacaa taggatttat atgaggaagt ttcttattct
    11041 tagcaaccct tgttgtcatt gcttctatcg aactcataaa tttatttggg ttcatgccta
    11101 gacttctaaa tgcctctcta tatggtataa tttcttctga ttcctttact tttttatctt
    11161 tgaagtaatc ttctactcta tctatactta tatctaaaag gttgcttatt ctttctattt
    11221 ctttagtatt atctattcct tttgcaacta ctacaccaaa gcatacattt tctaatttat
    11281 caaaaacttc tttttctact ataaatttca ttttgcttta tcctcctgtt attttacatt
    11341 attttctaaa tagattttat cactttgtgt cttataaata taccccctat ttgctaattg
    11401 tttaattttt ttaaattttc caattaatat tatatttatt cttatcagat attttatatg
    11461 tactaattac aaatttaact ttccaaaaca aatatcttgt tttataatgt ataatatatt
    11521 tttatggagg caagactgtg aaaaatttta ttctaatata ttttatagtg attaattcta
    11581 ttgctttctt ttcaatgtac atagataaga aaagagcaat tagaaacgaa tggagaataa
    11641 aagaagctac tcttatgtct atagcagtaa ttggaggtag tataggttct atgattggaa
    11701 tgtattcttt tagacataaa actaaacaca ttaaatttac atttggtata ccatttatac
    11761 tatttttaca atttttatta gtatattttt atattttaaa gtaattgaaa tataaaacct
    11821 tattcttttc aataaattaa ggtgtataaa attaaaaact ttatacacct ttttctagca
    11881 attattaaaa agatatttaa ttaaaattaa tgacccttat gtttagcttt tctcttttct
    11941 tgtttcaaat taaatattct ttctttttct tcttcttttc tatttttagc taggtttttt
    12001 ctatcctgtt ttctttcttc atattgaagc ttaagagcat tttgagcctt agtccctata
    12061 gtttcttttt cttgttcctt ttttactttt ctctgcattc ttttgtatcc aatgttgtct
    12121 ttggtcacat ttttttctaa tttaatgcta ccaaaatcta atgagaaaaa tctttcaagt
    12181 ataaattcat acacttctac ttctttaggt tctgctccaa aaactaccct acatgcttca
    12241 tattttttac catcttgtct ttcaaatata cctacccaaa atggttcttc aaataagact
    12301 gttaattttc cactaatttt gtccatcgct agttcctcct taaattactt tctaaagaac
    12361 ggacaaccaa ggaggcaggt tactgacagc tattgctgcg tctggactac caaccagact
    12421 gtgtttttat ctttatagat taaatattat cttttttaat catgcttgtc aatcttagta
    12481 tcaacatcta tcatattgat ttaagtgaaa tcaagctttt taactctact ttattattgt
    12541 taacactaag aaaattaatt atttttcata ataaattagt tattatactt atttaaatat
    12601 caatctttat tgttccctgc ataaacaaga ctgcttcacc agaaattttt acagtattat
    12661 ccttcaacac ttcacactga attataccac cacgattaga aagttgagca gctaccattt
    12721 tgtgttttcc caacttttca gaccagtatg gaataaaatt acaatgtgat gaaccagtaa
    12781 cagggtcttc tgaatctaat tctggacaaa aatatcttga tacaaaatct gtattacttc
    12841 cttgtggtgt gattattata cctaaccagt ctgtcaattt acgcaattgt gcgtaattag
    12901 gtttataatt tataacttct tgttcactgt ttagcaataa gattaaatct cttgaagaat
    12961 agacagcact tggtacacag cctatcaaat tagcttgtta tggtgataac tctatttcaa
    13021 taggcattat ttctggaaat atcatttcat atagatttcc atttcgtgta acttcaagct
    13081 ttccactttg agtaaagaaa tctatccttt ttacattaac atcaataaaa tttgatatta
    13141 cataagctgc tgctaaagtt gcatgaccac ataaatcaat ttctgcttta ggagtaaacc
    13201 atcgtaattc ataatttcct ttacctttaa ctacaaatgc tgtttctggt aaattgtttt
    13261 cttctgctat tttctgcatt aactctaaag gtattattct gtcaagaaca caaactccag
    13321 caggatttcc tttaaatagt ttagttgcaa atgaatcaac tatgtagtat tccattattt
    13381 gacctctctt tccaacataa aatatacatc aatttacatt caataaaact ttaaatttct
    13441 tacatatatt ttttaatatt gctctgctca acaacttttt tacctaacaa tgtaattgct
    13501 ttttttgcac aattatttac acaacgataa cacattgtac attgcccatt tgcaacaatc
    13561 atacaatttt taattactag attcttcatt ggacataaat taacacattt accacataca
    13621 atacattcct cagtattaat ttttagttta tctgtataca tatttgtttt attgataaaa
    13681 tacaatcgtt gtccaaataa ccctacaata tgactcaaaa aacctaaccc ttcttttggt
    13741 ggtgacccat gtttcagttt tttagcagct ttagttactt tcttttctgc ttttattaca
    13801 agctttttat tatactctaa agaatgtttt aatacctttt catctcctat actatctggc
    13861 atcttcacat gtaatccacc aacagttata gcaccgtatt tttttaatat tctagctaac
    13921 acaccagagc catctccact aaatagagcc atagttgcaa taataaaaac acgtttacca
    13981 ttccacaaag ttgaatgatt tattataaaa tctttcaata tttttggcac attactatat
    14041 tgcacaggat aactaaaaat aatattctca tgattattga tgtgctgtaa aagttcatta
    14101 tcttcaatag aataagaatt tgctgttata tcatattctt gtaaaaattt ttctacacag
    14161 tatcttgaat ttcctgttcc actaaaatat attcctatca tagtacttct cctaatagct
    14221 aagattatat agctctattc ttaaaattct gactttttat attatctaaa ttaagtagtt
    14281 tatttaaata atataaactt aattcaacaa gttacctact gaacctccca tgactaaagt
    14341 catagggttc ctgcttgata gaattttaca taatcaattt actaaatact tgtctaattc
    14401 tttttttatc attttaaata tacattttaa taatattagt aagctatact tccttaagca
    14461 tatattttcc ctaaagtaaa actattgatg attattccat tatataacct aattataaat
    14521 ttagtaagtt catcacatac tataccctta attctcaatt ataaagttcc ctaaaaaata
    14581 tttgaaaatt taactatgaa aagatttcct attgcctaat atgtataagt aaggagtact
    14641 atatagtact ccttatattg tactcttata acattgatac cttaatatta ttttaatctt
    14701 aatcattatt gatttgattt aaaattataa ataggttcca atattttaat tatgtcaact
    14761 atatctctta tagttttaat aatctcatca attggtttat gtgcttgttg cactttatca
    14821 atagtattaa ttaaatccat ttacacatct agcacctgta tggcaattta gcattatcct
    14881 tatcttatac tttgttgtaa attcttgatt acataattct attatctaac ttatagctgt
    14941 gcaatctaca ttatctgtaa atatctttgc tttatatatt ttccctgaac ctctaacatt
    15001 aatataacag cctttttgct atacactttt aactatatac ttaattttaa taacattact
    15061 tgtttataaa aatatactat agataaattt cacataaaaa taataattca agtattgatt
    15121 tttatataag aaaatttgta atgtactaaa aataacataa aagtttccat ttttcaatat
    15181 gtaagctttt cttatgttat tttattgtta aaggtttcaa gtttttattt attcatttta
    15241 aaaaaattat tatttttgta aaactaatcc catagtcata ctatcgtcag aatatgcttc
    15301 acccttaaca ttactaaaaa actcaacaga cttaaatcca atattttcaa cttctaataa
    15361 gaaactttct tttgaaaaat agtgattcca aacataaaat acattttcat ctttttcggt
    15421 tattacaaga gtttgttcta atgtattata atcattgtat ttacaattat cttgtagaca
    15481 catatatttt tcattactcc aaaatcctcc atcttcattt atttcccaat atttaatttc
    15541 ctcaaaatta ttgtatttat ttattgtaaa tacatctaat attaattttc ctccaggttt
    15601 taaactatca taaatttttt ccattaataa ccttctattt tcagttgaca aagctccata
    15661 atcacaataa attaaagtag ccaaatcaaa ttcatcatta tagttcatat ttaaataact
    15721 ttgaaacaag taatttatgt ttaaattagt ctccttattt ctattttgtg catagttaat
    15781 agaacgtttt gagaaatcaa ttccagttac cttataacct ttttgtgcaa atttctctgc
    15841 atataaacct ggaccacaac ctaaatcaag taaattagga taattaactg gatttgcaac
    15901 tgtaactatc caatttacag aatcctcgat aaagttaaaa tttctacttg ctccttcaaa
    15961 attagtgtct aaatgtgctt ttaataattg ttttgaaata tactcatcat cccaaaaatt
    16021 aatttcactt tgtttataaa gctctggtct ttctaaatac atacataatt tgtttaacaa
    16081 aacataccac tccttaaaat ttatttttaa aataaaaaaa ccacagagtt tactctgtgg
    16141 tttaacttca aaaagatgag tttaaataag agtaaaatag aaccaaatat ttttaagcac
    16201 aacaataaaa ctatagcttt actttgtttg cttattaaat ttgattttat tcactctttt
    16261 ccagcaatac ttatatcaac gattttatga tatcaaatat caccgaacaa catcatcctt
    16321 tcatattttt attttatgaa tcaattatat atttaaattt tatatttgtc aataaatttt
    16381 aattttaata aattttaatc atcatagtaa aaaaaaattt tttatataaa actaatcaaa
    16441 aaaagatatc tgtccatctc ctgagttttt taacttttcc ttcattattt tagagtaact
    16501 gcttgataga gaatatttat atctaagttc attaaccatt ttatacaatt gatttttata
    16561 ctccctatta agtgaacctg cactatatag tattgataat ttatcaaata attcaggaaa
    16621 ctctttttta acaaagtcaa aaaaaacacc tctagttata ccccttaagt ataaagtccc
    16681 aggtaaaaca taatgtacat tacattcttt tgcatgtcta aatagcgaat ttatattatc
    16741 aaaatcatct gtaatatatg gtataattgg cataatatgt agaccaactg atgcgttagt
    16801 ttttctaaat tcttttaaca ttttaaatcg tctcattgaa tccacaccat ttggctcaat
    16861 aagcctttgt gtatcttcat ttactgtggt aattgtagat gctacattga tatatgtaat
    16921 cctagatagc ttatcaatta aatcgtaatc tcttaaaatt aaatctgact ttgtagaaat
    16981 aatagctggt gtcttgtatt ttatcagcag gtttaatatt tctggcataa ttttatagtc
    17041 agcttctatt ggttgataac tatctgtaac tcctcctata ttgattactt ctttcttcca
    17101 tttactgctt ttaagttgtc tctcaagttt ttctactata tttgttttta cataaatctc
    17161 ttcaaaataa ttattggagt taatgtactt atgagagtaa atagcataac aatatttaca
    17221 tccatgctca catccacgat aaatatttaa atcccattta tatggcatac tcctttttaa
    17281 tttattacaa gctatttcac aatttattgg aatatatttt attttattat acaaataatc
    17341 acctctttat atacaataat actataatgt taatctatag taattaaaac atgatatttc
    17401 cagaccacaa ttcataatct ttactatatt tacaaagtaa tatattccat tcatttattg
    17461 gtattcctcc acatgttgct ttttcttcta aattatataa gcattgcttt aaatacattt
    17521 tattatgcca atcatcatat aaattttcta aatttaaact atgttcatta gatatatcat
    17581 ttgcaaaatt tctatttttc cattttaata tacttgtata tttaagttca ttaggtttat
    17641 aatttctttt taacatttca tcatgtacaa tttttgtata aaatttaaat tcatctatac
    17701 tataattcaa tactttattc actaatagat ggtttggagt acctttttta tctatagaac
    17761 cttttatagc aagtagctct ctccattgac ttactaattg atttttaggt aatacatcaa
    17821 ttaaatcctt atgccataat ctcataattt aactccttac tataaaatat tttataaatt
    17881 caataaccaa tctatcttac aattttacat gttcaataat tttatctgta aaaactatac
    17941 catttaaatg gtctatctca tgacaaagac attttgccat aaaacctgaa cctttaattt
    18001 ctattttctc accgtattcg tttagagctt gcactgtaac tttttttggt ctttttaatt
    18061 taccccatac ttctggaaaa cttaaacatc cttcaacaac aatttgttcc ccttcttgtt
    18121 taataatttc aggattaaca agcttaataa gtccttctcc taaatctata acaactaatc
    18181 gctttaatac tcctacttgg catgcagcta atcctcctcc atttggagta ttgtacatgg
    18241 tctctgccat gtcatttaat atttctctta tcttattatc tacgttttct acatacttac
    18301 tcttttttct taaaatttca tcatcaaatg ttcttatctt tcttatagcc ataatattct
    18361 cccctactta tctgtgctct tgattttctt tcactttctt caaataaaaa tacactttca
    18421 attggttcat taaaaattct tgaaatatca tatgccaacc atattgaagg ttcattttta
    18481 ccagtttcta tagcatttat agcctgtcta gatgttccaa ctaatcttcc tagttgttct
    18541 tgtgtcaatc ctaatttttc tcttaatatc tttattttat ttttcatatt tgctccattc
    18601 atgtaaggtt aactttacat cttaaatgta acatttttca acattactgt caagtcaacc
    18661 ttacatttat atattaaatt ttcttattta actatttttt tactaaactt tttaaataat
    18721 aataaaatcc atattaatga tataacaatt gaaataaaca tatagatata attatcttcc
    18781 ttaaatgcct tcgatatcca aaaagttggt aagaagaaca gaatatattg tgctttattt
    18841 aaaataaaaa atggtgctaa aatacccatc ataaaaacac ctgagaattt cgtaattacc
    18901 attccttcta gtttatttga tgataaagaa atcactagta ttgatataat aactccttgt
    18961 aataaaacta ataaagaaat tgataagttt aacaagaaag aaattttagt tagagaaaaa
    19021 aagataagta gaatcatagt aataataaat gctaaggtac ctggtattcc aaatcgtgaa
    19081 ataagataac cagttttacc aagcggagtt actattaaat atcttgaaat gctgtcatca
    19141 atttctccca atatgaccat tgtagaagca aaaaagaaca taaaaggtgt aataaaagct
    19201 aaaagtaaat caaacatcaa gaaatacggc tctaaattta attgataata aaacttgtta
    19261 agtgatatat tctgtattaa tggtattcca aattttataa atactccaca taatattgga
    19321 gaaacacata ctataaaaag catagcatct ctcttaattt gttcaaactc ttgtttaaac
    19381 gcatttataa tctttatcat agttttactc cacctacctt tttccaactg ctacaaataa
    19441 aataataagt tataaaataa attattatta taattgatat taaaatatta atcattataa
    19501 aatttttatc tccactaata agagaaatac atatattaaa agggtacaaa tttgcatatg
    19561 attttgtgtc aagaagtaca ttgagtattg gtggaataaa gcatataatc tcaataggaa
    19621 tacttaaaac aataaactga ttcaaactag atgctttact tgcaagtatc aatccaagca
    19681 aagaaaatat aatagaagaa aaaaaggtac ctatagttga tattataata ttatctaaat
    19741 ttaatgtaat agcaataaac atagctacaa tactagatat tactcctaat gaaattacct
    19801 ttgataaaat atactcagat acttttactg gagatacagc taaggaattt aaaacccttt
    19861 gacttttctc aagaagtaca atagctccca taaaaaacaa tcccattgct gctgggtcag
    19921 aataaatcat aattatagct attttttctc tcataaaagt tggaaataca ttgataatac
    19981 aaatatatat aatacttaaa aaaagatata aaaaataaaa accatactta aattgaaact
    20041 ttatatctcc taaaataaga ttttttagcc tcattgtaaa ttcctccctg ttatctcaat
    20101 aaaaatatca tttaatgttg gttcactact atgtattgat aaaagtttat ttttcttaat
    20161 aagcatattc aaatttttat cattagcagt attgttcaga aaacattcag aagttttttc
    20221 tccattatca aagtaagtat atctaacttt aatagctcct tttgacataa taagattatg
    20281 tggtgtatct aaagcagata ttttaccatt tacaatgaac gctactctgt cacaaagctc
    20341 cgttgcatct aacatgttat gagttgttaa aataatagtt ttacctttag atttttctga
    20401 taaaattata tctttcataa ccttgctatt tgacgggtca agaccacttg ttggttcatc
    20461 taaaaataaa atatctggat tatgtaataa agcttttata aaatttaatc gagatttcat
    20521 accttttgaa tactcagata cccttttatt tgcttcattt tctagtccta ctgattttaa
    20581 taactcatca atagatagta gttttttaga atatagagac ccaaaatatt ttaaattttc
    20641 tattgcagtc aacttttcat ataaactagg aaattcaaaa tcaactccta tattttcata
    20701 aaatttattt gagtgtcttt tactctctac tccatttaca atgacacttc ctccataatt
    20761 agttatcatt ccaattagaa tcttttgtaa tgtagatttt cctgcacctg atggtcctaa
    20821 aaatcctagt atttcccctt tttctacttc aaagttaata ttttgtaaaa aatttctatc
    20881 tgtataacta aatgataaat catcaacttt aatcattcaa tatcaccctt tcaaattttt
    20941 gactattttg actatatggt caataaaata taattttttt gtttgttaat aatttttaat
    21001 aaaagttatt agcaaacaaa atttaaattc tattccataa gttgtataac taatccgttt
    21061 atcattaatc ttaaagcatc ttcaaaacaa ttacttccaa cctcgcgctt atatagcatg
    21121 gtaagaaaaa ctactctaaa tgctccacta aagctttcta tgtctttatt ttttgcattt
    21181 ggtatgcaag atataatctc tccaatttca aagtcatcat gtctaaagtg ttcttcaatt
    21241 atagaatctg gtaactttct caccaatatc tcaaattctc catttatcat gagatttaaa
    21301 attgaagtac tatcaaccat cctatagaat ttcataaata tatctgtcaa ttgttcacat
    21361 gtaatacttt catctactct atttatctca tataagattt ctttttgtat agagtcatgt
    21421 atatctctca atacctcaaa aaagagcaat tcttttgatt gaaaaaatag ataaaaagtt
    21481 ccttttggta tttttacccg ttttacaagc tcatcaacag tagtttttct aataccatat
    21541 tgctccatac acaatcttgc ttcttcttta agtcttttta ttatatactc tctttcttta
    21601 tcagaatacg atttaggcat ctttttcctc cagtttgact aaaataattt atatagtcaa
    21661 tataatactt tttctatcta atgtcaatag tattattagt atatttaaaa ataagcttaa
    21721 aaactttcga aaattctgaa ttttatatag cattttttat tatctagtgt tataattaag
    21781 atataaataa tatattattc taattttcat atctatttgc ttatttttta acttagtttc
    21841 taatagaagt actcatattt atctttttaa aagtttcaac tttaataaaa aataaaggag
    21901 ttgattttat ggctactact acaaaaaacc gtttttacga ttatgttcaa gaaaacttaa
    21961 ataaatatgt tgaagtacat tcttacttta gcagttgtcc tctatatggt gaaattatag
    22021 aagcagatag cttatcaatt acactttgct caagatatat agatgaatct aatccaactt
    22081 taaatgaaac ttacacatta tatttaccat taagttcaat aatatctatt agaacactat
    22141 aaaaagttaa ttaaat
//
LOCUS       DAMJVX010000047        21977 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMJVX010000047 DAMJVX010000000
VERSION     DAMJVX010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.049"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(85..1191)
                     /locus_tag="PC407_001664"
     CDS             complement(85..1191)
                     /locus_tag="PC407_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2497114.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVTTEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DPLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHSMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(1226..2767)
                     /locus_tag="PC407_001665"
     CDS             complement(1226..2767)
                     /locus_tag="PC407_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2497115.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            complement(3129..3875)
                     /locus_tag="PC407_001666"
     CDS             complement(3129..3875)
                     /locus_tag="PC407_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2497116.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCNSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            complement(4087..5397)
                     /locus_tag="PC407_001667"
     CDS             complement(4087..5397)
                     /locus_tag="PC407_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2497117.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            complement(5410..5880)
                     /locus_tag="PC407_001668"
     CDS             complement(5410..5880)
                     /locus_tag="PC407_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497118.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            complement(5884..6735)
                     /locus_tag="PC407_001669"
     CDS             complement(5884..6735)
                     /locus_tag="PC407_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2497119.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            7660..8571
                     /locus_tag="PC407_001670"
     CDS             7660..8571
                     /locus_tag="PC407_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2497120.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            8746..9243
                     /locus_tag="PC407_001671"
     CDS             8746..9243
                     /locus_tag="PC407_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDF2497121.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNAIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVILAGKYAKFVIVGGQKEVGDFWFKFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
     gene            9987..10349
                     /locus_tag="PC407_001672"
     CDS             9987..10349
                     /locus_tag="PC407_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2497122.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVNNVLK"
     gene            10415..11032
                     /locus_tag="PC407_001673"
     CDS             10415..11032
                     /locus_tag="PC407_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HDF2497123.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            11029..11514
                     /locus_tag="PC407_001674"
     CDS             11029..11514
                     /locus_tag="PC407_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HDF2497124.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            11557..12075
                     /locus_tag="PC407_001675"
     CDS             11557..12075
                     /locus_tag="PC407_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HDF2497125.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            12211..13170
                     /locus_tag="PC407_001676"
     CDS             12211..13170
                     /locus_tag="PC407_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2497126.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNTRTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            13196..14899
                     /locus_tag="PC407_001677"
     CDS             13196..14899
                     /locus_tag="PC407_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDF2497127.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINRV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            15156..17258
                     /locus_tag="PC407_001678"
     CDS             15156..17258
                     /locus_tag="PC407_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDF2497128.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESTDEKEAIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKNVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQINEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMHGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            17271..17711
                     /locus_tag="PC407_001679"
     CDS             17271..17711
                     /locus_tag="PC407_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDF2497129.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            17747..18550
                     /locus_tag="PC407_001680"
     CDS             17747..18550
                     /locus_tag="PC407_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDF2497130.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKSFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            18590..19186
                     /locus_tag="PC407_001681"
     CDS             18590..19186
                     /locus_tag="PC407_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HDF2497131.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGLFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            19292..20155
                     /locus_tag="PC407_001682"
     CDS             19292..20155
                     /locus_tag="PC407_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HDF2497132.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            20222..21211
                     /locus_tag="PC407_001683"
     CDS             20222..21211
                     /locus_tag="PC407_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDF2497133.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NRESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            21303..21914
                     /locus_tag="PC407_001684"
     CDS             21303..21914
                     /locus_tag="PC407_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2497134.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         8525 a         2597 c         3703 g         7152 t
ORIGIN      
        1 gtaaaacaac tttaatatat aacttataaa gttgaagata cataggtttt tatttttata
       61 atttaaagag gtatattatg atatttataa tttaatcata tgataaacta ttttggcagt
      121 atcatataat tcgtctatat ttacatattc attaactgtg tgtacatcat acatacctag
      181 acttaatata gcacaattat atccaagatt agctaatata tttgcgtcac ttccaccacc
      241 aattaccatt ggatttggaa caacaccaac ttgtcttatt gcttcttcac ttaacttgaa
      301 aacgtgacta tctctactaa gttcaaaact agggtaagac atactatgtt caaaggtata
      361 agtagtatta aatttagatg cagcatcctt acaacatttt tccatatgat taagctcata
      421 ctctaaagtt tcaggtatat gagacctaat ttcagcagtc aatgtaacct tatcagttac
      481 tatgtttgta gctccaccac cttctatttt acctatattt gaagtagtca atggatctat
      541 tctgcctatg tgcatattgc ttattgcatg agaggctact aatatagcat ttaacccttt
      601 ttcaggttct ataccagcat gtgctttctt tccatgaaaa gagattttta tagattgttg
      661 agcaggagct ttgtatgcta tcgaaccaat agctccacca gaatctaaaa tcaccatatc
      721 tttacaaggc aagttgtcag gattaaagtt tttgacacca tgcataccag cttcctcaca
      781 gattgtaaaa caaagataaa tatctctatg tggtattttt tctgtagtaa catgttctaa
      841 agcttcaagt atagcagcta ttccagcttt atcatctgca ccaagtgtag tagttccatc
      901 acttctaact acattaccat ctagtattgg ttttacacct aaacaaggtt gaacttggtc
      961 catgtgtgca cataaacaaa gtggtctact tccttcttca ccttttatat aggcaatgac
     1021 attacctgta ttaccaccat atttttcacc tgcatcatct ataatggcat ctatatttct
     1081 ttcttttaag tagtttacca accacttaga catttctaat tcttgattag atgggctatc
     1141 aattctaacc atatccataa aattatttat aagtctattt ttatctaaca taaattattt
     1201 ctccttttag aaagatttaa ataaattaca tgctatatga aggaagtatt ccaggtccta
     1261 gtggaatatt aaataccata aacaatatta atagtatagt ccacactatt aagaatgata
     1321 tagagtatgg tatcatatta gcaattacag tacccatacc agcatcttta tcgtattttc
     1381 ttgtgaatgc tagtataata gggaagtacg gaaataatgg gctaagtgga tttgttatac
     1441 aatcacctat acgataagcc atctgagtaa gtgctggatt gtatccaacc atcataaaca
     1501 ttggtacaaa tataggtgca agaatagccc actttgcaga tgcactacct ataaataaat
     1561 ttataaaaca tgataatata ataaatccta tcaacaataa aggtccattt attcctgctg
     1621 attttaaaaa ttctccgccc tttacagcta gtatcagacc taaattacta tttgtgaata
     1681 attgcaaaaa ttgtgaagca gcaaatgcta gtattatata tccacccata tcactcattg
     1741 cactagccat tatagagact acatccttat ccttctttat tttacctata gtttttccgt
     1801 atacaacacc aggtatgaaa aatgctagtg ctattatagg taccatacct ttcataagag
     1861 gagcattgtt tgatagtaaa tctcctgttt ctgggtcagc taagaatggt tttttaccga
     1921 ttacactaag agctactata atagctacat atattaatat acttatacca gcttttttaa
     1981 gccctttctt ttctacatca ctaagacttc catcaacatc tagttctgca tttccttcat
     2041 atttaccaaa tctaggagct acaatttttt ctgttaccca tgttgccaaa agtattagaa
     2101 caaaagttga tgctatcata aaaaataagt tcattgttgc attggcttga aatccagaat
     2161 caatcatttg agctgctgga actgtaaaac ttgcagcaag tatatcattg acacttatca
     2221 taatgtttgc ggcaaatcct agacagactg ctgcatatcc agaaaataaa cctataagtg
     2281 gatgtctacc aacaccaaga tatacaagtg cagctaatgg tggaaataat attgtacctg
     2341 catctgaagc tatattggca agcataccta tagtcattac aacaagagtt actcttgttt
     2401 caggaatctt gcttactgat gctttgattg ttgctgtcat aagtcctgtt ttgtcacata
     2461 caccagcacc tatcatagtt actagaacta gacccaaagg agcaaaccct tggaaattgc
     2521 taactatgtt tcctaaaaga atttgaagct gttctacagt caataaattt actgcagtta
     2581 tagtttctcc agtaccagga tgaactactg atatccccat agaaccaact agactagata
     2641 gtataagtaa aagtacacat aaccctaaaa atattgtaac tgggtctggt agtttattac
     2701 caacagtttc tattctgttt aatgtcctgt caaaaaagtt tttcttctta actacgttat
     2761 cattcataat atcctcccta atgtttaaaa atataattaa aaaataagta ttgtggttga
     2821 tcttacaata taaattctgc ttgtcgattt aagacgtgtc cacaatatgt cctaaaaaaa
     2881 gtataaaata aactttttcc tgtgtcaatg cagttttctg aattttctgg ttgagtggtg
     2941 ttagttaaat atgctttaag tagattaatg acgaattata tctaatgtat ttaaatagca
     3001 tatgcacttt aaagttaatt ttaaaacaaa attttatgaa aagctagtgg aagattaagt
     3061 tattaaaata agtagcgtag taaagtgtta gattttttaa ttaatcaaag aaatttactt
     3121 tgtattttct acaaaaatct gtgaatatct tttgaattat gtagaggata taaatttatt
     3181 ttatttacat agattagatt ttgttctaat ttactgtatt caaatggttt tccatgtcca
     3241 ggataaatca ttttaggttt tgtattgatg attgttttcc aagattgaat aaattctagt
     3301 ttgctttctg caaatatggt aattttgtga gtacttggaa atccattcat tgcagaatct
     3361 ccacaaaata aaacaccatc atcaattagt agtgaaattg aatcagcagt atgtcctggt
     3421 gtatctataa tttttccatt tagttctttt tcaatttctt tacgattctt gtcagacaca
     3481 agaatacacc ttttttcaaa ttcttgttta agtggagaaa aactatgctc accttttcca
     3541 aatagtttca taatttggca aaagagaaag gcagtacggc tgttacaacc accaataaaa
     3601 gagttttgac ctttatatag aatttctaaa gctttatcat gcataacaat ctttgtatta
     3661 ggagattcgt ctagaatctg atttagaaaa ccaacatggt catcatgagc atgagttaga
     3721 aatatgtatc gaatatcttt gatttttatg ttgaatttag aaattcgttt tttgaaatgt
     3781 ttaaaaccat tttcatatcc agtatcaatc aagacaaagc catcttgaat agggtacaac
     3841 catgtattta taattcgatt tcctgcatta aacatttttt caccaccttg aattagaata
     3901 taaatttata ttaattagaa agaatatagc atagttctac tgatatttta acgtatctct
     3961 aaaagtttta aggaggataa tatattttag tttaatctaa aagctaaaag aaaagaagta
     4021 tactgttaca aaaacaaaga gtggtataac ttttacagta gataccaccc tattataaaa
     4081 atagacttat aaagttttat cgtgtttttc taggaatgtt agatattctt ctttagtcat
     4141 aactggagta aaggtatcta atatatcttt agcatcctta gcattatcaa aaagaaggtc
     4201 tacaactgta agtgccattg ctttagcagg aattatgtaa gctaaatctt catcaactat
     4261 ttcaaaatct cttgtatgaa gagctccttt tacaccacca atcatagggt gaagagtagg
     4321 cattatatga gacacatctc caaaatcaaa agagcctgta aaatctccac catcaataac
     4381 ttttcctttt agacctaaat cttcaagatt gtttttaaat aaattatcaa gacttttgta
     4441 tcgtaatatt ggaagatatc caggaatttc agttatctca acatcagctc caactgccat
     4501 tccaccagcc atcaaagcct tatttattct ttcattagca tcaatcatac catttatagt
     4561 tctagctcta acatatgatt ccatatgaac atcagcagga acaacattta ctatatctcc
     4621 accttttgtg atgatagggt ggaatctaac tctttcagac tctttaaaag tttctctaag
     4681 tgcattcacg ttgtttatag caagcattgc tgcatttaaa gcatttattc catcatgtgg
     4741 agcagaacca gcatgagatt ctttacctat aaatttaacc ttttttccaa caaatccatt
     4801 actctcagga ccaactagag ccttatcttc tcctaaatca gaagcatgaa acatcataga
     4861 tatgtctata tcatcaaaat aacctctttt tattaattct tgttttccac caaaataagt
     4921 tatttttcct tcttttttta attgctctct gtattccaac tcaataaact cttcagcagg
     4981 agtagccata aaagatactt taccatctaa atgttctaaa acgccacttt ttactaaacc
     5041 aacagcagca cctaacattc cagctatttg aatattatgt ccacaagtat gagaagcacc
     5101 tatttcatta gcatctttat gttctttaca tgatatacca tccaattctc caagtataga
     5161 tatatgagga cctttcttgc tagaatttgc atctgctttg catcctgtaa ctgctatatt
     5221 tttttctaca gataaattta attcttgctc aaaaaagttt gaaacagctt ctgtagtttt
     5281 aaattcttta tatccatatt ctggattttt gtatattcct tgacctattt ttaatatttt
     5341 ttctctattt tcatctatag tttttattac tagagatttt aattgttctt tattcataat
     5401 aaattcctcc tatattttac cttgaatact taaaactata tgagctatac tcgcacatgc
     5461 aaagaatata gttgtaaata caataagtga aattataact attttccatg acatttcttt
     5521 taaagcttct attttgtttc caacagaaat tcctgcaaaa gctagtagtg gagtagttat
     5581 agccataaag ttaatattat tagtgttagt taagaatact tcagatgttg gactatatgg
     5641 catactaagt aaaaggccga ttatagttgc aaatccaaaa gctggaaaga ttgatttagg
     5701 aaataaatct tttataatca tagctgccat agcagctaat attagcatca ccataccagg
     5761 aagagctgta actatagatg taccaacgct aattctttgt cctataagaa ttataactcc
     5821 aataacagac ataaccaata attgatatga acttttcttt aaatttaaat tagcaaacat
     5881 ttattagcac tctccttctg tagatttaat ttctttagtt tttttatttc tcgaaaattt
     5941 aggttctaaa atcttataca taaaattagt aaatggtaag gatacaaaaa ctaagaatat
     6001 aattcctgtt atactagaca tcatattgct tgttgcagca taggcaagta ttgtatctgc
     6061 catttcaggt ggtaccatgg tagataatga accagcagct gctgtcatca tacttccact
     6121 accaacacca caggccattc ccaaagcata tgggtgaaga ccactataaa tagatattga
     6181 acctagtaca ctaaagtata tagtaccaag aactgttccc attaaatatg taccaagtac
     6241 accactacct tctggtgagt ttatcccata tttttctgat ataactccta aagaaggttc
     6301 acgacttata cttgcagtag ctccaacagc ttctcttttc attccaaaca ttaatgcaag
     6361 aggtaatgca acgattggtg caagtatatg accaaattct tgagcgataa atgctggtcc
     6421 agcttgaatt attttgtcaa tgtttggtcc aactgtagta ccatatttta taccaagcat
     6481 tactagtgca attccaacaa cttcaccagc aatatttatt tctttttctc caaccacttt
     6541 tttaagtgct ttaatttttt tacctaataa atctggtgtt attataagtc caattataac
     6601 tgcataaagc atagggaaca gtactataga ggcttttcca atagctatag ttctttgccc
     6661 tataaattca catattacta ccaagattaa gcaagttaaa aatacttgaa taaaatgttt
     6721 tttattaatt tccatgattt cctccccaaa agaataaata ttttacttat ctgaaatgag
     6781 aaaatacgtt ttcttaagta taatacgttt tcctaaataa aatgaaaaaa ctatttcaaa
     6841 taagtttcag aaatgtcaat aacatgtcct taattaagga taaaacaaaa atttaacatt
     6901 gtcaacgaaa aatatctaaa atattacata ttttgaatat attgtaatat agctttatta
     6961 tgaaaaaatt gtttaaatat tgtaggaata atagctaaaa agctttaaaa ttagtaatat
     7021 aaaattgaaa aataataata tagagttgag aaatagtaaa ttgaaatttt aaaataaatg
     7081 agtggaagta gcttagtaaa gtatggaaat gaatattaca atagaaaata aataatatgt
     7141 aaagacttta aattaatttt aagtattaag tacaaatact aatttttgat atagaaattg
     7201 aataaagtaa atgataataa gcaaatgaat taagatgata ataagtgaat tgatatagaa
     7261 ataagtaaat taagtaagat gataacaaat gaaatgataa taagtgaatt gatatagcaa
     7321 taagtaaatt aagtaagacg ataacaaatg aaatgataat aagtgaattg atatagcaat
     7381 aagtaaatta agtaagacga taacaaatga gatgataata agtgaattga tatagaaata
     7441 agtaaattaa gtaagatgat aacaaatgag atgataataa gtaaattgat atagtaataa
     7501 ataaattaag atgataatat ccaaagtgag aaagataata gaaagtaaat aaaatcatgt
     7561 aagaataatt tagataagat tctaagattt tatttattta aaaattagta tgattaattt
     7621 agattaaata aatttaataa ttttagtagg tggtaatgaa tgaaaataaa tagattaaca
     7681 gaaataatag taattctatt aaataaaaaa ttagtaacag ctaaagagtt ggcagatagg
     7741 tttgaagttt ctacaagaac catatataga gatatagaaa ctctgtctat gtcgggagta
     7801 ccagtatata tgacgaaagg caaaggagga gggatatctt taatagaaga atattctata
     7861 gacaaggcca tactgtcaaa aaaagataaa gagagtttaa tagttgcact gaaaacatta
     7921 caggcgacta agtatccaga aataaattca gtagtaaata aaattggctc aatatttgga
     7981 gagcagaatt tttcaaactg gattgaaata gactttactg aatggggaag taattttaat
     8041 gaagatgata aatttacaaa aataaaagaa gctattttaa gacgtaatac tataaatttt
     8101 aattatgtaa atagtttgtc aagccagaca aacagaactg tcgaaccact gaagcttatg
     8161 tataagagta agacttggta cttgtatgga ttttgtaaac taaaagatga ttttaggata
     8221 tttagaatta gtaggataag gaatttatct ataaaggatg aagtattttc aagaaagata
     8281 atagaagaag tatgtttgaa tgattctaaa gtaattaagg aaaatactat aacgttgaaa
     8341 ttgaggttta aagaaaagat gctttttaga gtatttgatg attttaataa ggatttaata
     8401 actaaaaatg aagatgatac atatgatgta attacagaat ttcctattgg tgaatggatt
     8461 tatggatata ttttatcgtt tggtgacaat gttgaagtac ttgaacctaa agatgttagg
     8521 gataatgtaa taactagact aagggagttg tctaaaattt attcattata atatgatgct
     8581 agattaaaga ttttattaaa tatataatat cttgcattta gtatataata tctcactgag
     8641 tttaattata ttaaaaatat gacataaagg tgtcgtatat tataaattat aattgaatta
     8701 gttcaaaata aaatatatta ataaaaacaa agtgaggtat aaagtatgaa ttatgaaata
     8761 gtagagataa atgagaaaat agttatagga atatcgaagg agactacaaa taaagatgga
     8821 caagcggtaa atgctatagg tgaattgtgg aaaaaattta tgggcaaagg gatatataat
     8881 gctataaagg gtaaaaagaa tgacaagaca ataggtctat atacagatta ccagggtgat
     8941 tttacaagtc cgtatagctt tgttgcatgt tgtgaagtaa atagtaattc tgataaagag
     9001 aagaatttag aagaattaaa cactaataat actgttggag aaagtattat tagtaaagtt
     9061 atactagcag gtaagtatgc aaagtttgtt atagtaggag gtcaaaaaga agtaggagat
     9121 ttttggttta agttttggca aatggatttt gataggactt atataagtga ttttgaagag
     9181 tatcaatgta atacttttga tacaaagaaa caagaaattc atatttacat aggaataaag
     9241 tagatttgtt attactatat tgaatgaagt attgagtata gtacatttat tatagatagt
     9301 tttactattt ataactataa gcagtaaatc tgtaaaagat ttactgcttt tttagtaatt
     9361 aatagtatca gtatcacttt gaatcttcag acatattcgt catttaatat tgtttattga
     9421 taaatgatac ttgtaaatat aatgatttgt tatttaattt aaaatatgaa aaaaagtatt
     9481 ataaacacat tagaatactt ctggatttat gagtatacaa tttgaaaaat ataaaaaata
     9541 atgtataaag ctaaatctaa tttaatcgat aatacatata gagaaatata attaagtaaa
     9601 taaacaacta tctattagat attgagaaag tcaaatccta tatgaatcta cagaaatgat
     9661 tttaatgaat ctgaaaaaat tttttataca aacataaaaa aggtataaag ttgttttatt
     9721 ataatttgat tacttttgat aactttttat gttttataca attatatttt taataactcg
     9781 tcaaaacgcc aaacatatat tacaatagat atataagttt ttttgttgac aaattatgtt
     9841 aagagaatta agtataatat ggcataaata attactttta tttatcaatg aaatgtattt
     9901 attgtgtagc gaaaaatagt cagatagtat agacaataac tgaagataaa agcgtttatt
     9961 gttgtacata tggaggagtt agttttatgg aaaataaaat tttgatagta gatgatgcta
    10021 tttttatgag aatgataatt aaagaagctc tgagtaaaag tggctataat aatttaatag
    10081 aagcaaatga tggagaagaa gcgtgtaata tatttaaata tgaaaagcct gatttgatgt
    10141 tgcttgatat tacaatgcca aaaaaagatg gtttagaggt gctaagagaa ataaaaaagt
    10201 tggattcaag tgctaaggtt gtaatgtgct ctgcaatagg acaagagaat atgattgtag
    10261 aagccataaa acttggagcg ttagatttta tagtgaagcc cttcaaaaca gaaacattag
    10321 ttaaagttgt aaataatgtt ttaaaataaa agtgtaaata tacactgata caatgaaaag
    10381 caaataaatg caataataaa agggaggggt ttatattata aattatttag aacttgatga
    10441 tgttcatata gatgctctta gagaaattgg taatataggc tctgggaatg caattactgc
    10501 acttgcatcc atgataaata gcaatgtaga agtattaata cctatggtca agatacttga
    10561 atataatgaa gcgacaaatt tgcttggtgg tccagaaaac aaagtagtat gtattttact
    10621 cgatatgaaa ggtgatatca atggaatgtt tatgtttttg ttggatgaat caataactca
    10681 acttatgcta agttctcttt ttaataaaga agaagctttt ttagatgaga ttgaggcaat
    10741 tgaaatttca gcaattaagg aaataggaaa tattatggca agttcatatg taaatgctat
    10801 tgcatctatg ttaaacatga ctataagtgt ttcaattcca gatatatgta ttgatatggt
    10861 tggagctgtt ctaaatgtgc ctatgataag attttcagat gttggtgaca aagtcttatt
    10921 tatagaaaat aaatttaaaa tgagtgataa ttattttact agccacattt tgatgattcc
    10981 agaaatgagc tcacttagag aaatactcgt gaggttggga ttagaagtat gaatagagag
    11041 atagtagtga acattgcaga tatgaagata gcctatagac caaatgtttt agttacttat
    11101 gcacttggct cttgtgtagg agtatgtttg atagataaag tagctggaat tggtggaatg
    11161 ttacacgtta tgttgccata tagtaaagat gctattaaca ttgaaaataa gtgtaaattt
    11221 gctgacacag gaataaatga gctcattaaa tctatggaga atataggtgc aaatagaata
    11281 tatataaaag caaaaattgc tggaggtgct cagatgtttt taggaagcag caattcagta
    11341 attgcaagta taggacatag gaatgttgag gcagtaaaaa aaactctttt aggattaaat
    11401 attccaatat tagctgagga cacaggtatg aattatggta gaacaatacg tttttacact
    11461 gagacgggtg aattacttgt agattcaata aataaaggta agaagaaatt ataatatata
    11521 tttcatagaa agtgtatttt aggagggagg tattaaatga gatttccaga tgacccatat
    11581 ttagattatg gactaattga tgaaggaatt aagtatctaa agtttaaagt agatgagcaa
    11641 gattttgggg ttgaacttga aaaggtaata gaaattactg gaaatcaaga aattatattt
    11701 attccagaac tacctacata ttctaaaggc ataataaatc taagaggaaa aatcattcca
    11761 ataatagata tgcgtttgag acttaaaaaa gatgaattaa tgactacaaa ttgcatgtat
    11821 atagtagtaa ctgaaataaa ggacttggta gttggatttg tggttgatag agttgatgat
    11881 atagtaagct tagaaaaaag taaaatatca cctccaccaa gagttactat agaatattcg
    11941 gattattttg taagtggcgt tggagaatta gaaggtaaaa taattacttt actagattta
    12001 gagaaacttt tgacagataa agatgaagtt ttaatagatg aattaatcaa tgggtttgat
    12061 tgcactatca tgtaacatag ggatgtagtt ggagtgttat tataaaaaga ttgtgatgag
    12121 aacatttaag aatatagtgg tataattatt aagtataaaa attttaaatc attaaaattt
    12181 gaatatatta tgtttaagag aggtttgtat atgaaaaata agcaacaggg aacaatttta
    12241 gtcgttgatg atagtgttct gatgtgtgac ttaatagata aaacacttac aaaagataat
    12301 tttaaggtta aaaaagcatt taatgcaata gaggcaaaag aatttattga agaatttata
    12361 ccagacatta ttttgcttga tataatttta ccagataact ctggttttga gttttgtaag
    12421 gaagtaaaat ctaatactcg tacagcagat atacctataa tttttataac ttcaaaaaat
    12481 acagatacag acatagtaaa aggatttggt gtaggagcaa ttgactatat ggtgaaacct
    12541 tttagtatga cagaattaaa agctcgtgtt atagctcatc ttgaagtaaa gaaatctcaa
    12601 gataaattaa aaaagataaa taatgaactg gaatcttctc ttgaaaaatt gaatagactc
    12661 gttgtaagag attatcttac aggtctttac aataggcgac atataattaa tcagcttatg
    12721 gagcaaagaa gaattaataa atatagtgaa acatcaatat cccttatcct tggtgatatt
    12781 gatgacttta aggttatcaa tgatacttat gggcatgaag caggggattt cgtgttaaca
    12841 gtaatatcaa gtataataaa aaagaattgt agacaaattg atactgtatc aagatggggt
    12901 ggagaagagt ttttgataat gctcacaaat actccaatag aaggtgctaa gatactttca
    12961 gaaagaatcc gtaaagagat aaaggagttt gatttcaact ataaagaaca taaaataaag
    13021 tgcactatta cattaggagt agtagaagta aactcaaata tatcattaga agaaaacata
    13081 tccttagcag ataaagcaat gtatgagggt aaaaacttag gaaaagattg tagcgtagtg
    13141 agtacaatga aaggattaaa aaagatataa acttacttta tgaaggagta cagatatgaa
    13201 aagagaagta aagctttcta aattagcaat tgtattgctg attattatag tattggctgt
    13261 tctagcaggg attttttctt ctatttcatc ttataaagca tttgaaaaag tagaaaaaag
    13321 tattaatgat atgaataatt tgaggactca aagtcagtat ataaaagata caactagaga
    13381 gataagtgat gtattaagaa gatatgtatt tacaggagaa aaaaagaata aagatattta
    13441 tgaaaaagaa ttatacggaa aaacaagaga atcagcttta aacaaattag aaaaaattgg
    13501 tcttacaaag agcgagtcaa ataaaatctt agaagcaaaa gaaatatcag attctttagt
    13561 accaacagaa caaagtgcaa taaaggcagt cgaaagtaat gataagagta aagctcaaag
    13621 tataattttt ggtagtgact atataaactc ccaagagaaa atggattctt taataaatga
    13681 atttcaagag gatatagtta cccgttcaaa ttccaaaatt gtagaagtaa aaaaagaatt
    13741 acaaagtcta atattaagaa caatatttat actttccctt ttaataatat tgacaataat
    13801 agaatataca gtaataaaat taaagatagt gattcctatt aatagagtag aaaaacactt
    13861 ttcaagaatt gcatctgggg acttaagaac aagtatagac gttgaagaaa gtaaatctga
    13921 aataggacta ttagtgagtt ctgtcaaaaa gatgcaaagt atgcttttaa attacataga
    13981 ccttatagac agtacattaa ctagtattgc aaaaggagat ttaagagtag agatagacca
    14041 agaatttata ggtgatttta aatcaattaa aatatcttta gaaagtataa taaactcttt
    14101 taatggtgat ttttctgaaa taaatttatc agcagaacaa gttgcaagtg cttcagaaca
    14161 agttgcagca ggtgcacaag ctctttcaca gggtgctact gaacaagcta gttcagtaga
    14221 agaattagct tccacgatta atgagatatc agatggaatt aagaagtgtg cagatagtgc
    14281 tagtcttgca tgtgaggtat ctgaccaatc tgcatcacaa gttggttttg gaagtagatg
    14341 tatggatgat atgatgacat caatgggtga aataagtgaa aaatcgagtg aaatatcaaa
    14401 aataatcaaa acaatagatg agatagcatt ccaaactaat attctcgcat taaatgcagc
    14461 tgttgaagca gcaagagcag gtcaatatgg tagaggattt gcagttgtag cagatgaagt
    14521 taggtctttg gctggtaaaa gtgcaaaagc agctcaaaat acagctattc tcatagaaga
    14581 agctattaaa acagtagaaa atggtgtcag aattgctaaa gagactgcaa atgcattgga
    14641 cttggtagta aatggtgtag aaaaaactac taattatatt aatggtattt cttcggctgt
    14701 acatagtcaa gaagtagcaa ttacacagat tttatctgga atagagcaaa tttccatagt
    14761 agtccaaatg aactcagcaa cagcagagga aagtgcagca gcctcagaag agatgtctgc
    14821 tcaagctcaa attttaaaag aattagtaga aaaatttcat ctaaaggaaa gtgaagtaag
    14881 agaatcatca tgggtttaaa ttatgaggtg aatattgaag taagagaatt atcatgtatt
    14941 taaattatga ggtgaatatt gaagtaagag aattatcatg tatttaaatt atgagatgta
    15001 tatatattga aataagagaa tgattgtgta tttaaattat gaggtacata tagtgagata
    15061 agagaatcat caaggattta aattatgaga tgcatatata atagaaaatt atttgtaggt
    15121 cgtaggtgat aaatgtgaat tatattgaac cgagtatgag acaaatgtta gatatgttta
    15181 tatttgagac aagtttatta ctcgaacaat tagatgaaat tatgattcaa atagaaaaag
    15241 agaaagtatt tactcataac aacattaatg aaacttttag aataatgcat acgataaaag
    15301 gttcagcatc tatgatgggg ttagacaata tatctgcttt agcacatagt gtagaagact
    15361 tgttttatgc tatacgtgaa ggtaaaatag aagttttaga caatgaccta ttatttgatt
    15421 tagtctttca atcattagat tttataagaa gagatataga tgagctacaa tcaagaggtt
    15481 gtatatcttc tgattactct aattttattg aaggaattga agagtatgtg agtactttgg
    15541 aattatcaag ttctatgaaa tcaaaggata atgcaaattt agaaagtaca gacgaaaaag
    15601 aagcaatcga ttcaaatttt gaatacaaag aagactctga tgtagtaaaa ataatctttg
    15661 aacaaggttg catgatggaa aatgtgagag cgttcatgct tgtatttgaa cttagagatt
    15721 actgtgaatt tatagatttt tatccaaaag atgtagaaac aaatgcagag tctacagaat
    15781 atattaaaag ttatgggttt tatatacaat ttgtagctaa agaggataag gcaatagttt
    15841 ataaaactat agaagataat ctctatgtaa aagaatacat aaatattagt aaaaaagaaa
    15901 tagaaatgaa aaatacatca aataatgatg cagtagaaaa taaattaaaa gataataata
    15961 aagtaagtaa aagttatcaa aataactata cagaagatga gatacaagaa gatgttttaa
    16021 atgaaaaaac tgatatggaa aaagatagtc taagtgaaga aaatgaacat ttaccaataa
    16081 gtaagcagag tattatgagt gtaaatttag aaaaattgga tttacttcaa aatatagttg
    16141 gagaaattgt aattatggaa tcaatggttg taaatagcgt aaattctaca ggtgttgata
    16201 tagataattt caataaggca acaagacaat tacacaagct tacagatgaa ttgcaagata
    16261 tagtaatgtc tatgcgtatg attcaaattg ctggtgtatt tcaaaaaatg catagaattg
    16321 tacgagatat gaataaaaaa ttagataaaa atgtagagtt aataactata ggagcagaga
    16381 cagaggtaga taagaatgta attgataatt tagcagaacc ttttatgcac cttataagaa
    16441 atgctatgga ccatgcaata gagcctattg aggagcgtat agcgaaaggt aaagaagaag
    16501 tgggaaagat agttttatct gctgagaatc taggcggaga agctgtgata agtatttcag
    16561 atgatggtag aggcattatg agagataaga ttttggcaaa agctaaggaa caaggtcttc
    16621 ttaaacatca aattaatgag tatactgatg acgaggtcaa ttctttgatt atgactccag
    16681 gtttttctac aaaagaatct ataactgaat tttctggaag aggagtaggc atggacattg
    16741 ttcgtaaaag tattgaaaaa gttggaggtt caattgaagt aaaaagtaaa caggatgaag
    16801 ggacaacctt tactataaaa ataccattaa ctctttctat agtagagggt atgaagttta
    16861 aagttggaga ttcactattt actttaccaa tatcatccat aagaagaaca tttaaactta
    16921 cagacttaaa tcaaataatt acagaaggaa ataagacaga gttaattaca atatatggag
    16981 catgtattcc cattataagg ttacataaaa tatataatat tcaaacaaaa gtaaccaatc
    17041 tgatgcatgg taatatgata attattgaaa atgagcataa agcagtatgc atttttgttg
    17101 atgaaataat aggtaatcag caagttgttg tgaaaccatt tccagtttat ttcaaccgat
    17161 ttaatataaa aaataagggt ttatcgggat gtacaatact tggggatgga agtataagtt
    17221 taattataga tgcagatagc attatagaaa tatattaggg gatttaaagt atggctaaag
    17281 atgaaattat taaacaagta ttaacatttt atgtaaatga tgttatttat ggaatagaat
    17341 tagaaaatgt gattgaaacc ataagatttc aatcaataac gtatgtacca tgtcttccaa
    17401 catatataag tggagttatt aatttgagag gaagaataat accagttatt aatatgcata
    17461 taaagtacaa tcttcctaaa gctgattata atgagagaac ttgtataata attacaaagg
    17521 ttgatgagta tcaagttgga attattgtag ataaagttgt tgatgttatt catatagaaa
    17581 atctaaattt actagagacg actaattcta acaacactaa tattaataaa gatataaaag
    17641 agattgcgaa agttgaagat aattctatat taattttaga tattcgaaag tttttgatta
    17701 atagcatata gttagttctg ataaattagc aggaggaagt ttttgcatga taaaactcac
    17761 agatgaagaa tttatagttc ttgtaaacca tgtaaaaaaa gaatatggta ttgatttaag
    17821 taaaaaaaga gcgttaattg aaggtcgctt atacaataca atgatagaga aaaaacttag
    17881 ttcttttagt caatatatga atctattatt taaagataag acaggaaatg aagctataaa
    17941 cctaataaat agattgagta ctaaccacac attttttatg agagaacctc aacattttga
    18001 gtttatacag aatagtatac ttcctttttg ggaagaaaat aataaaataa gaaacataaa
    18061 tatatggagt gcaggatgtt ctagtggaga agaagcgtat tcaatagcta tgactttgga
    18121 tgattatttt ggatataata aagagttgtg gaacattaaa atcatagcca cagatatttc
    18181 aactaactca cttgaaaaag ctaaaagagg tatatacata gaatcaaatg ttaacaatgt
    18241 tcctgagtta tggaagaaga agtattttat agacaacaag gatggtacat ttaaaatttg
    18301 tgataagatt agaaaaagtg taatttttaa gtcatttaat ttaatgaatg atttctcata
    18361 tcaacatttt gatttgattt tttgtagaaa tgttatgatt tattttgatt taaaaacaag
    18421 agaagaactt ataaataaat tttataatgc taccaaaaaa gatagttttt tgtttgtagg
    18481 acatgcagaa gtaatcaaca gaagtagtac taaatacaaa tatgtaaaac ctgctatata
    18541 taaacgataa aaaggtattt ttgatatatg attttttgga ggcggtaatg tggaacgttt
    18601 taataatatg gttattgcta taggcgcatc tgtaggtggt acagaagcaa tacttgaaat
    18661 tataaaagac ctaccaaaat caacaccagg gattgttgta gttcaacata tgccagctat
    18721 atttacttat atgtatgcac aacggcttga caagcagtgt attatgaatg taagagaagc
    18781 taagaataat gatagagtcg aacaaggaaa tgtgttaatt gcacctgggg gttaccagat
    18841 gaaattgtgt acggataaac aaggatatta tgtaacttgt gaaaaaggag aaagaataag
    18901 tggtcactgt ccatcagtag acgttttgtt tgattcagta gcagaagtag caggtaaaaa
    18961 ttcaatagga ataatactta caggtatggg ttatgatgga gcaaatggac ttttaaaaat
    19021 gaaagaaaat ggtttattta caattggaca ggatgaaaat tcttgtatag tatatggaat
    19081 gccaatggta gcttttgata gaggttctgt tatgttacaa ttacctcttg gtgaaatttc
    19141 aaactgtttg ataagacata ttaatatttt aaaacaaaaa gattaatttt ttataaataa
    19201 aaattaatct tttattaaat tatatggata aaaaacccta gaaactccaa atataataag
    19261 agagatttat atttaggagg aaaatgataa tatgaaattt tttaaagtcg atacaaaagc
    19321 taaagtttta agatatgtat ttgttttaac attgactata atggctgttt tggcaattaa
    19381 acatgactat aaatataata atttaaggaa agatgcttca attaatttaa atagtaataa
    19441 ggaagcagtg actgttatga gtaaccagtc attaccaaag ggtaatacaa atagtaaaca
    19501 aactttatca gtaagtacaa taggaagtaa aacaatagtg aaaaatgatg agtatttaga
    19561 aagtactata aacatgcctg taataacaaa ttcaaataaa attgttgaaa ggtctacaaa
    19621 tgataagata aaaaatgaca tatttgaatt ttataataaa tcatataaag aagcaaagca
    19681 gtatcttaag gataatcctg acgagaaaaa taagtttgtt gcaaatgtag actttgaact
    19741 taaaaagaat actgattctg ctcttagtat aaaagtgaga tattatactt atagtggtgg
    19801 agcacatgga ttttatcaag atatagcata taatgtggat atgagaactg gaaaattttt
    19861 agaattaatg gacttattta aggataatac taaatataaa gaagttatag atgaagaaat
    19921 taaaagacaa atagctgaac ttgaaaaaaa ggatgaagaa aatatcggaa tatataattt
    19981 taaaggtata aaagagaatc aaaactttta cttgcaagat gagaatctag ttgtatattt
    20041 tgatttatat gatataactc catatgcagc gggaatacca gaattttcaa taaataaagc
    20101 gttgataagt tctatgctaa aaccagagta tgtagattta tttgatttga aatagaagat
    20161 taaattaaat gttatgctat aatatttagc atagaaactt attaaaattg ggggtataaa
    20221 aatgctactt ttaaaaaaag acgacataaa aaaagtattt acaatgagag atgcaattga
    20281 agctgataag gaagctttta gaatttattg tgaaggaaag agtgtaaatc ctttgagaac
    20341 caatatatct gttccaagtc aagatgctag tatgttattt atgccaggat acgtagaaga
    20401 attaggctgt gggggaataa aaattgtatc tgtatttcct aaaaatgctc aaaaaggcaa
    20461 accagtaatt ccagcaacag tactattatt agatggagaa actggagaag ttagtgcagt
    20521 attggatggt acttatgtaa ctcagattcg tactggagca gcctctggag cagctataga
    20581 tgtattggct aaaaaagatt ctaagattgg agctttgata ggtctaggag gacaaggtga
    20641 gccacaactt gaagccatga tagaagctag aaatttggat gaggtaagag tttttagtag
    20701 aaacagggaa agtagagaaa aatttgcaga agaaatgaac ttaaaattaa gtaaatataa
    20761 tactaaaata gttgcagttg aatcatctga tgaagccata gataacgcag atgtgatagt
    20821 tttagctact ccatcaaaac aaccagtact taatggaaac ttagtgaaaa aaggagcttt
    20881 aataagtgca gtaggttcat atatgccaga tatgcaagag ttgtgcccaa actgtttaac
    20941 tagagcgtct aagatatttt ttgaatcaac agatgcagtt ttatctgagt caggagatat
    21001 attaattcca ttaaaagaag gtaagataag taaagatgat tttagtggtg atttaggaaa
    21061 cgttataaat ggaactattc ctggaagaga agatgatgat gagataattg tatttaaaac
    21121 tgttgggata ggtgtacaag atgtcgtaac agcaaaaaga atatacgata aagcaaaaga
    21181 aaacggtatt ggaatagact ggaagtggta aaatattcaa aataattcta tagttagaca
    21241 aatttatgtt ataatgttga taaataaatt ttttattatt ttaattgagg gaggatgttt
    21301 gtatgaaaat ggtaaaaaag ttaatattgt ctacgattat ggctctagtt ttagttctaa
    21361 ctatagggtg ttctaatgca gaagaaaaag taaagtcgaa ttttgaagct aatttagctg
    21421 cattagaaat gtataagtat gatgaaaaag gaatttctga tgcattaaca gaagctgaat
    21481 ataaacagat tcaaacttat aacagcaagg acaaggtttc aatgattgct caattgacac
    21541 ctctttatgg taaagaaaat gctgaaaaga tatacgaaac aattgttaaa ttaattcaag
    21601 gtgttgaaaa gacagtaaca gtggaatctt ctagtaagga taagataact ttgaaagttg
    21661 taagtaaagc tttagatact agtaaagtaa tgagtgaagc gaacacttta gtaaaatctt
    21721 atgtaactga tgaaaagaat tacgataaaa aagaaaaaga agttcaagat gatatgacaa
    21781 aaatgattat agacctttac aataagaaac caactaagga agttacaact atgattacat
    21841 atactaaaaa agatggtaaa tggcaaccac ctagtaatat acaaaagtta tgtgaaggaa
    21901 tatatacaat ttaatttatt ttaaagacac aaaactatct tttaagcata taataaaatg
    21961 tttggaagat agtttat
//
LOCUS       DAMJVX010000048        21955 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMJVX010000048 DAMJVX010000000
VERSION     DAMJVX010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21955
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.050"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(193..900)
                     /locus_tag="PC407_001685"
     CDS             complement(193..900)
                     /locus_tag="PC407_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2497135.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYRWGATGPNAFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            complement(1099..3012)
                     /locus_tag="PC407_001686"
     CDS             complement(1099..3012)
                     /locus_tag="PC407_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2497136.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNSELKKVAVAIIDDDKDKIGRIIHNVKIVGTTSDIKAVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            complement(3096..4187)
                     /locus_tag="PC407_001687"
     CDS             complement(3096..4187)
                     /locus_tag="PC407_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2497137.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            complement(4193..5536)
                     /locus_tag="PC407_001688"
     CDS             complement(4193..5536)
                     /locus_tag="PC407_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HDF2497138.1"
                     /translation="MPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLAEVGNENVV
                     CVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVG
                     TPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNNVK
                     VELASNPEFLSQGTAVKDTLYASRIVIGVESEWAREILEEIYKPFNQPIIVTNRNSAE
                     MIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGS
                     CFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGL
                     TFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIE
                     DAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEYYSIGR"
     gene            complement(5514..6266)
                     /locus_tag="PC407_001689"
     CDS             complement(5514..6266)
                     /locus_tag="PC407_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2497139.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            complement(6314..7222)
                     /locus_tag="PC407_001690"
     CDS             complement(6314..7222)
                     /locus_tag="PC407_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2497140.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            complement(7244..7993)
                     /locus_tag="PC407_001691"
     CDS             complement(7244..7993)
                     /locus_tag="PC407_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2497141.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYKRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            complement(8022..9197)
                     /locus_tag="PC407_001692"
     CDS             complement(8022..9197)
                     /locus_tag="PC407_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDF2497142.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            complement(9212..10051)
                     /locus_tag="PC407_001693"
     CDS             complement(9212..10051)
                     /locus_tag="PC407_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2497143.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNEDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYTFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            complement(10054..11211)
                     /locus_tag="PC407_001694"
     CDS             complement(10054..11211)
                     /locus_tag="PC407_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HDF2497144.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNIDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTIFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            complement(11227..11949)
                     /locus_tag="PC407_001695"
     CDS             complement(11227..11949)
                     /locus_tag="PC407_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2497145.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            complement(11961..13034)
                     /locus_tag="PC407_001696"
     CDS             complement(11961..13034)
                     /locus_tag="PC407_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HDF2497146.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEYISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            complement(13056..14762)
                     /locus_tag="PC407_001697"
     CDS             complement(13056..14762)
                     /locus_tag="PC407_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2497147.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNDTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            complement(14804..16363)
                     /gene="murJ"
                     /locus_tag="PC407_001698"
     CDS             complement(14804..16363)
                     /gene="murJ"
                     /locus_tag="PC407_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2497148.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDDKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRRR"
     gene            complement(16478..17533)
                     /locus_tag="PC407_001699"
     CDS             complement(16478..17533)
                     /locus_tag="PC407_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HDF2497149.1"
                     /translation="MIKKISTILSLVLLISISSTIEVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGIKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTAKLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            complement(17628..18314)
                     /locus_tag="PC407_001700"
     CDS             complement(17628..18314)
                     /locus_tag="PC407_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDF2497150.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            complement(18534..20558)
                     /locus_tag="PC407_001701"
     CDS             complement(18534..20558)
                     /locus_tag="PC407_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2497151.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAGT
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            complement(20812..21870)
                     /locus_tag="PC407_001702"
     CDS             complement(20812..21870)
                     /locus_tag="PC407_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HDF2497152.1"
                     /translation="MIVCNNPGTWMRMYPQLRHAVWHGVTLADFAFPFFVISLGVTIP
                     ISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNPDLNSVRILGVLQRMGLVY
                     FVTSLVYLLLKKLNVGSTATIITFLCIATFIIVGYYILAKPYGFELEGSLAQLVDLHF
                     FKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENIGEYKKFFKILVMSIILLI
                     GAFIFNQYFPFNKRLWSSSFVLLMAGSYGMLLSIFYFICDIKNKSKIFTPIIALGSSP
                     IFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELITPWAGTTWDSLIFSLLYVL
                     FWVIVMSIMYKKKIFIKI"
BASE COUNT         6849 a         4055 c         2292 g         8759 t
ORIGIN      
        1 tatttgatat ttgattataa tatgaaataa tttattataa aaaaaacagc aaagaaattt
       61 atcttctttg ctgtttttat aacgttcatc actattttaa tattactaaa ctcttaaatc
      121 tctatgtaaa acattaaatg taattatata tttaatgtta atattcaatc ctatgtaaat
      181 caatatctat tattattgta aaacacgtgt agcagttata agtcttcttg cataattagt
      241 tcctggagcc atgctagtaa cttttactac atctcctgtt tgtggtgaat gtatgaattt
      301 agaatctcct acatacaatc caacatgatt tattgaacca cctgaaccaa agaaaactaa
      361 atctcctggt tttaattctg ctcttgatac tttctttcct actgttgctt gtgatcttga
      421 tactcttggt aaattaaccc ctgctccatt tttatagaca taagaagtga acccagaaca
      481 atcaaaagca tttggtccag ttgctcccca tctatatggc ttaccttgtt gcttaaatgc
      541 taagtttaaa acattttgta cctttgcatc tacatttcca ccttgaatag ttaaataatc
      601 tccatttacc catcctgtaa cattgtcaac actttttatt ttaatccatc catttgaatg
      661 taattctaaa acttcaactt tgtctcctgt gtttatcttt cctactactg catcatttgc
      721 actagctcca cttcttacat ttaaaaaatc acactggtct actgttgcac tcataggtga
      781 attagttggc tctgctgcac tagccgttat tgctcctgct tctactgtga caactcctgc
      841 tattaacacc atagctataa attttttcaa tatatgtttt ttatttttaa tattcatcat
      901 aatctcaaat acctaatcct ttcgaaaatt taacattttg ttccttcgtt tccaattcct
      961 aattaacatt ctatcttaca tcaaaataaa ttgcaacctt ttttgtaatc tttttgtaat
     1021 ctttttgtaa tcttttgttt ctttttagta tatatttgtt tctttttgct ccccctggtt
     1081 acaaattaga aatatattct acttttctct agttgttgca atttctttga tattagcatc
     1141 ttctggttta atataagttg gcactaattc cctcataata ccatctattt tttcaacctc
     1201 ttcattgtta gtaatttctc ttaaaagttt taagtatttt gttatttttt ctctatcaac
     1261 atctattggt ctaccaataa aaatcttttt atgctctgta tccaacagac cttcttctga
     1321 cataagtagt tcttcataca acttttctcc tggtctaaga ccagaaaatt caatttttat
     1381 atcaacatct ggctcaaatc cactgaattt aattaagttt tttgctaaat ccaatattct
     1441 tacaggttct cccatatcaa gcacaaatat ttctccacct ttagccatag ccccagcttg
     1501 tataactaag cctactgctt ctggtattgt cataaaatat ctaatgatat ctggatgagt
     1561 aactgtcact ggtccgccat cttcaatttg cttcttaaaa agtggaatta cactaccatt
     1621 actaccaagc acatttccaa atctaacagc aacaaattca gtctggcttt cagcattcat
     1681 agtctgtata atcatttcag cagctctttt agtagctccc attatgttag tagggtttac
     1741 agccttatct gtagatataa ggacaaattt cttagctcta tattttgaag acaagccagc
     1801 tatgtttaaa gttccaaata tattattctt tatagcttcg ccagggctcg attccataag
     1861 aggaacatgt ttatgtgctg ctgcatgaaa taccacttct ggtttatatt tattaaatat
     1921 ttcatccatt cttttttctt ctcttattga tgctattact gtctttaaat ctaatttatt
     1981 tttatatttt ctaagtaatt cttgttgtat tgagtaagca ttattttcat aattatcaac
     2041 tataataagc tctttaggat taaatcctgc aatctgtctg caaagctctg aaccaattga
     2101 gcctcctcct ccagttatta ggattacttt tccctcaatg taattactta tctctcttaa
     2161 atttgttttt ataggctctc tacccaacaa gtcttctatc tctacttctc taatctgttt
     2221 tatatcaact tttccatcta taatttcgta aattcctggt attgtcttta ttttacagtt
     2281 agtgttttta catatatcaa taatctcttt ttttctacgt ttctctatgt ttgctattga
     2341 aaagattatc tcatctacat tgtacttttc taccacagct tttatatccg aagttgttcc
     2401 tacaattttc acattatgga ttattcttcc aattttatct ttatcatcat caattatagc
     2461 cactgctact tttttaagtt ctgaatttct ttttagttcc tgtataacca tttctcctgc
     2521 atctcctgca ccaattataa ggatatttga aacctttcct ctagaatatg tcttgttcat
     2581 gactattctt atagctctat aagctattct tgcgccactt gtaaacatta taataaatat
     2641 tgtatttaat acataaaaca ttataggata atcaaaccct aataatttat ttattatata
     2701 aatcggaatt aaataagcta aagttgccac aaaaatagat ataatttctt cttctcctgc
     2761 atatctccat aatgtgttgt agcacttaaa caatctaaat gatactatat gaagtactat
     2821 atatatacac actgaaatta ttatatcatt agtggtaaat gcatttaaat tacctgtcaa
     2881 gcttaaagac attcgaaacg ctactagtac gctacaaata tctagaatta atagtgttaa
     2941 atgtctaaat ttatattttt taaaaagcct attaaaaaag ctttctttct ctaaatctat
     3001 actttctcgc ataactagaa gccctccttt aaacagcaaa agctacgcac ctaagcatag
     3061 ccaacaacat ctatcttttg ctgtattttt tcattttatt ttttatattt atcaaatata
     3121 gaatcataaa ttttttctat atcattaaag tttaaattaa cctcataatt ttctctaaca
     3181 aattgacgcc ctttcttacc catattaagt ctaagttcat cattttccat tagttttatt
     3241 atagcatttt ttatttcttc tggttgtttt ggttctacaa ttaagccagt ttcattttcc
     3301 catacagatt ctggatgtcc tcctacattt gtaactataa caggtacttc acatgcttct
     3361 gattctatag ctgccactcc aaatccctct cttaaagaag gaaatacagt tacatcaaaa
     3421 gaattaaaag tcttgcttac attttctgga gatattcttc ctaaaaactg aacatcattt
     3481 tctataccca attctttagc taaatttatt aggttgtcca tctgacttcc agagccacct
     3541 attttaagaa ttattttctt atctttatat tcatctttaa gcatcttaaa tgcttgtatt
     3601 aaatattcta ttccatattt tttttctaaa ctctttataa ttcctattac aaaagcgtct
     3661 tctttttcta cttccatagg cttaaaccta tccatatcta ctccaaaaaa ggttacatct
     3721 acatgtttag ctgtatattt attagcctct ttagccatat ctctactatt tgaaaagata
     3781 tagtctgctt ttctaaagtt atgttttata atttgttttt gtataaaacc attattaggg
     3841 aaatcataaa tatcagtacc ccaaacagaa ataatatatg gatgataccc aagtagactt
     3901 cctataaatc cataactact tgcatactgt gcatgcaata tatctggctt tatttcattt
     3961 actagcttct ttatctgctt tattgaacct aagtatcccg tttttttaga aatattttca
     4021 ttttttaatt cttctacatt agaaccaaaa ttataaattt ttattccttc catgtgacct
     4081 ccatttaaag agattacatg aatgtcatat cctttatttt taaaaaaact acaccatttt
     4141 aaagtatgag gatttgaccc atcagctaaa taacaaacag tcttcataat atttaccttc
     4201 ctatcgaata atactcaatt cctaactctt ccatatcttt aattttataa caatttcttc
     4261 catcaaggac taaaggattt ttcatctttt ccttatactt atgtatatca taatttaata
     4321 catctttcca ttctgtcatt ataaaacata tctctgcatc ttctatcgca tcatctacag
     4381 aatcagcata aagtatagag tcaccaaata ttttcttagt attatttaca ccaattgggt
     4441 cgtatacagt aactcttgcc cctctttcaa gcaaatattt tatatttggt atagatggtg
     4501 cttctcttat atcatcagtg tctggtttaa aagtcaaacc taaaacagca actttcatat
     4561 cttgaaagct ctctactaac ttagatgcct ttctaactaa tttaagtttt tgactttcat
     4621 taacttctat tgcagctttt actgttttta attcataacc attatgttca gataaccagt
     4681 gaagagcttt tgtgtctttt ggaaagcaag aaccgccata ccctattccc gcctttaaaa
     4741 acttacttcc tattctatca tcataactca ttcctaaagc tacatcttct atgttagctc
     4801 ctgctatttc acaaagattt gcaatatcat tcataaatga tattttaaga gctaagaagt
     4861 tatttgatgc gtattttatc atctcagcac tatttctatt tgtaactatt ataggttgat
     4921 taaaaggctt atagatttct tctaatattt ctctagccca ttcactttct acacctataa
     4981 cgattcttga agcatataga gtatctttta ctgctgttcc ttgtgataaa aactctggat
     5041 tagatgcaag ctctactttt acattatttt ttaggtttct atttatataa tcttctatct
     5101 cttcatttgt accaattgga actgtagatt ttattactac tagagtatct tttgtcacag
     5161 tttcagctat ttgctttgca actgccttta tataatctag gttggctgag ccatcacttc
     5221 tctcaggtgt acctacagct attaatatta catctacatc ttcataagct tttttatagt
     5281 ctgtagtgta atctattctt cctctgttgt aatttttaac cataagttct tctaggcctt
     5341 cttcatatat aggtgctatt ccaagtttca ttgatttaac tttttgttca tctacatcaa
     5401 cacagactac attttcattg cctacctctg ctagacaaac acctgtaacc aaacctacat
     5461 atcccgtccc agctatagct attttcattt ttttacctcc aagtatataa aacttatact
     5521 ctttttttaa tggcattaaa aacatagaaa caaaatacat atagactttt tggtaaactt
     5581 aaattttcaa catttctata agtattccaa gttcgtttta atgcttttat cttgttactt
     5641 gatatagaat ttccaactaa tctgtaattc actaaatcct cctgaatccc atatgcatat
     5701 ttttcctttt taagtaactg taaccaggtc gcagtatctt gtcctcttct taccaaaggc
     5761 atgctgaact ctcctacaat ctccttatca atgaccactg tagaacatcc tattatagta
     5821 tttctaagta atccttcata gtcaattttc ttaggagctc ttgcaacctt attcgttttt
     5881 gacccatctt gtctcatata tctataagat gtaaaagaaa aacctacatt ttcttttaac
     5941 atatattcta tctgaatctc taattttcta ctatcccata aatcatcact atctacaaat
     6001 gcaataaatc gaccttccgc attcttaata ccaacatttc tagatactgc agctccagaa
     6061 tttttttcaa gctttatata ttttattcta gcatcttctt ttcgaaaact ttttataatt
     6121 tcacttgaat tgtccttaga acaatcatcc actaataaaa gctgccaatt tttataagtt
     6181 tgattttgga ttgactttat cgtctctgat aaaaactctt ctgaattata aacaggagta
     6241 attatcgata ctaaaggctc attcatattt ttactctcct tatatagcaa ctagcagtac
     6301 tataccaaca agtttactta gatactaatt ttctttgtcc aatatatttg taatatccta
     6361 atgggaataa tgctaaatac aacaatttat cagatacttt tcttaattca ccaaaataat
     6421 gactatcaga caacaatgag tatctggcta aatttatgta attttttatc cacatatatg
     6481 gatatctact taatggatat gtattttgat ttattaaata taaaaactct aatgctctac
     6541 ctcttaatgc atttttactt aatatattct tagtcaaacc atcatccaaa taatctctta
     6601 ttatcatagg tttatttaca catataactt tatagtattt agacatttca ttccatacta
     6661 cactttctgg taaaaatctc acttcttttc tttctggaaa aggaaatttt ataagctcat
     6721 cagttacaaa tactatgctt ttatctccct taacgccata cttaaaatat aggtctgcaa
     6781 agtgacatat tttcttatct tcaggcattt ctgtccctat caaatctcca cttggatata
     6841 cacaaagtcc aacaactcca ctaaatcctt ctttattctc taaagaatcc cactcatcat
     6901 tagcaatttc aagagaatct ttaattaaac catcatcact atctacaatg aaaaatagct
     6961 ctccatctgc attttttaca cctagattta ttgcagtatg ttttcctcca ttttctttgt
     7021 atatatatct tatatttaat ttattttctg atataaattt ttcaactaac tctttagtac
     7081 tgtcgctact tccatcatca acaatcaacc attcaaaatc attgaaatca taagtttgag
     7141 cttttaaatc attgtacaaa ttttcaagta aatgagctct gttatacgtc ggagtaaata
     7201 cagtaaattt atatttatac attaattaaa tcttcctctc tgtctaactt tttttgattc
     7261 cgtttattcc ataattaata aagtagtata tagctttgta taaaggtatt ttttctatat
     7321 ttctatatat attccaagtc cacatagcag cttttatttt attaccactt agggaattgc
     7381 tagattttct atataatgct agaacttcat ttattccatg agcaatatgc ccttttttca
     7441 atatcgataa ccataaaaca tagtcttcat gtcttacacc actcattctt atttcaaaat
     7501 ccaatttaga cttatcaatc acaacagtaa gacatcctaa gatatttcct tttaataatc
     7561 ttttataatc tacattaggt ggaactttta ttactttgtt taatttttta tcattttcat
     7621 ccatcagttc atatgaagta aatgaaatta catagtcatt ttctaacata aaatttactt
     7681 gtttttctaa cttactgcta ttccattggt catcactatc taaaaaagct ataaattgtc
     7741 ctgttgcctt acttagtgct aaatttctag cattagagac acccttatta gtctcagtct
     7801 ttatacattt tattctacta tcctgttgca tataagattt gactatatta ggactattat
     7861 ctgttgagca atcatcaata attaacattt cccattcttg ataagtttgg tttaatactg
     7921 attttatggt tgcttcaata aatttttcag aattatacat gggagtaatt atagatacta
     7981 aattcttctt catattacct tcctcatcat ttataaaata tttatttaat tttacttaat
     8041 acacaatcat atatcctctt gcagttctta ttatcattat atctgaaaaa cttatttctt
     8101 tcattttttt ggttatccat aacctcaaaa ttattttcaa taatatcaat aagtttattc
     8161 acggcttcat cattattata agccaaagac ccaaacattt cactttttaa atctatatat
     8221 gaatcaactt tttccatata ttcatccaaa tcgaattggt aaaacaagat aggtctattc
     8281 atatacaaaa agtctatggc tacacttgaa taatcagtta tatttgcagc tgattttctg
     8341 atttcatttg ctagagttac ccctttatct aaaaacacaa ttcgttttcc tgttatattt
     8401 tcttttatat catctataaa ctcatgcata agatgatgca tataaatttt tacatttata
     8461 tctttctcat ctaatatttt atttaatttg tcatcattta aaaaattcat tatattttta
     8521 aaataatctg tatccgtaaa ttttcctgaa ctaaatttta tccaatctct ccatgtagga
     8581 gtatataata tatctcgagt ttgtgcaact tcatttgttg gtaaattgtc atatcttgca
     8641 ttcccaatta tataaactga gtcatctaac attccccaat catttaccat aacatttctt
     8701 tcaaattcag aacttgctgt aactatatta tagcttttat ataggttatc tataatattt
     8761 ttgaactttt tgttcatagg ttttcttttt ttaaagctta tagtcccatg atttaaaaac
     8821 acttttatcg tttttttatg aaaataatta agaacaggta cagcaaaatt gtatggtgct
     8881 atatctgctg atggtgcatg tgaaaatact atagcttttg aattgtaata atacagatag
     8941 ttttctacac ttcctcttat taattttttg cctggaattt tatcaaatac tttactgtct
     9001 ttatttacta cccaataagt ttctacatca ttatgttctt ttagcatata ctcataaaaa
     9061 aactttgaat tatcattgta tatatcccct gaatgtccac ctataagcca gatatctcta
     9121 ccattaaact tatgcttatt aaatggatat ataaaaaaag ccaaaagcat atttattaaa
     9181 aatttaacct tgtccataaa caccatcctc attataactt gtactgtcta aatccattaa
     9241 ttcttccttt tatagtggca ctatgataat ctctattgga actcaatata agacccccta
     9301 tatttcttac aagttttgat accataactt ttgcaaacgt ataatttttt ctatagacta
     9361 tttccttttt gcaaagcgct ccaaagcctc ttccataatt ataatctttt tgatactttt
     9421 ctttagattt agaatgtttt tttgcaggat ggtaaattat atcatttcca aagtattttc
     9481 ctttaaaacc taaactcaat aagtttaaaa cataatctat ctcttcacca gcaccaaact
     9541 ctccaccaac acccaatttt tcatcaaatc ttgtataatc tttaccttca aaatcaataa
     9601 aaaaagttat ggatgttata gtatccaaaa cgttggaact tgtgatatca caagttccat
     9661 catacatttt cttaaatgca tcaactttat ttgaatccat agttttgcaa ctgtatattt
     9721 tataatcttc atttttatta aagaaattta taactttttc taatgtatca ttttcgtaga
     9781 cacaatcatc atcagggaaa cctataattt gccctacagc attatctatg cctacatttc
     9841 tgttataact caatcctttt ttagagcttt ttatatactt tatatctaac ttatcaatgt
     9901 atttatcaac tatttcttta actttgctat tatcattttg gtctactact ataagttcaa
     9961 agtttttgta agtttgattt tctaagctat ccataagtaa gtatatatca tcatatctat
    10021 ttaatgttgg cattattaat gatattaaca ttactacctc ctacttttca tcttccacaa
    10081 atctatttcc cttttatcat ttaatatcac ttcttttatt agtaatacta ctgcaaaact
    10141 aaatgaagct tctaataata aattttctgc aaaagagtat agtaaaaata taaataaagc
    10201 tactaaatat gcttttttat ctttttctat aaagctttct attcctttat acatcaaata
    10261 cataaataat atacaagcaa ctactcctag tacactaatt atatatacat aagaactatc
    10321 taatggatta gttgctttta tagcaggact atatgaattt ccaaaaggtt ttagaaaatt
    10381 ccccttttca gccaagtata catgccagaa cttaggtcta cttgctaata atctatttaa
    10441 taaagtgctt ttatcaaata ttgtaccaat aattacacta aatagagtaa gtattattgg
    10501 taatgtcttt aggagcattg gcagtccttt tatttttttt atatctatcc atcttattat
    10561 ttctacaaat attaatcctg ctaatatagt gaaaaatcca gttctactat atgtggtttg
    10621 ataaacaaaa aatgcagaac caaataaaat tattctatcc catttattat aatctttata
    10681 tcttaagaat atatatgctg catatatagt aaccatatga taaaatacac tatttggatt
    10741 accaagtcca aaatccattc ttgtaacaag catatctatt ttttctatat atctatagtg
    10801 gacttcctga ttaaatatta atgaaggaaa taaattaaat aataatatag ctaccattaa
    10861 aaaacatgcc gatattatta aataattttt aactataaat ttatcatcta tttgcttagc
    10921 tgatacagct aataaaacga ttaataatat agctccttcc ctatttaata aataaagtaa
    10981 gacacaaact ccaactagta ccatttctgt tatacttttt ctcttatcaa ataaaaaaag
    11041 tagcattagt attcctattg aagaataaaa taacgctgat ttaatcatcc ctatatctat
    11101 atttgggtca ataaatgata tcatatactt tctaagtaat atgataaaca aaacaaatat
    11161 ataaagatat tttctaatca taatatttct atcatcattt aaagtcttca ttttactttc
    11221 tcccttttag cttcgtaaag tattacttgc aaatatttta caacacttcc atatcttatt
    11281 tacacctgtt aagtgtttgc tttctaagaa ataatttctc tttttagccc aactttttcc
    11341 ccaccaatgt atcccaaaag tgttttctgt tatacattct ggtgtaaagt cttctttaaa
    11401 tccaaaagga taaaaatatt ctttgtcgta aacacgtatt ccttcttcat aattatttgg
    11461 gtcaatcttc ttataattct tttccataag atgagtcatt atttttggta ttgtaaacaa
    11521 tgaagaagta agtacttctt tttcataata ctccaataag tcttttaaaa aaggatgtcc
    11581 tttttctaca cctattatag caccatttat atactctcta tcatcttcat acccacagat
    11641 tagcctatta ttttctaaaa gaggagttat atcctttaat atttgcatat ctgtatccat
    11701 ataaactcca ccttgttcat agagtacttt tattcttgta taatcagata tgaatgccca
    11761 aagttttctt ttataacatt cttccaaaaa cttatttcct tttatttctt tctctatatc
    11821 aaagtttttt tcattccact ctactatctc atattctgga agcttttctt tccaagaatt
    11881 tatacatata ttttctatat ttccttttgg accaccaaac catacataat gtattttttt
    11941 aggaatcaaa tgaacacctc ctacatatat tcttgtctat cttcttcttt taactgttct
    12001 actatttttt tgacatcttg agctttatct tttctacaaa ccataactgc atcattcgta
    12061 gatactacta tcaaatcact aatacctatt gttgatatta atttgtcatc tgaatatata
    12121 attgaatttt tagtatctat agtaatattt tcacctcttt tgatgttacc ttcatcatca
    12181 gttggatata ttgcacctag agaatcccaa gaaccaacat cattccatcc aaaatcacca
    12241 ggaactacta taacatcatt acttctttcc ataataccat aatctataga gattgattgt
    12301 atagttgggt atatttcttt tattttttcc atttcttctt ttgttcctaa gtatttactt
    12361 atatattcta atttttcata tacttttggc aagtatctct taaaatcttc taatatttta
    12421 gataccttcc atacgaacat tccactattc catacatact ttttagagtt tacatattct
    12481 ttagcaattt catagtttgg tttttctaca aactccacta cctcatatgc aacatcttct
    12541 atcgtatttt ctctattaaa atttatgtat ccatatcctg ttgatggaaa tgtaggagtt
    12601 atcccaatag ttactagttt atcattattt tctgctatat agatagcttt ttccaaaata
    12661 gatttaaatt ctttttcatc ttttatataa tggtcagctg gatatacaca cataacacca
    12721 tcaccatatt ttttcattat gttaaatgct gcaaagccta tagctgctgc tgtatttctg
    12781 gcacatggct ctggcaatat attactatcc aaacatttat cttttactat atcctttaaa
    12841 gcttccaatt gcttctcatt ggttactata aataaatcat ctttttttgc tagagaatct
    12901 attctgttta tagtttcgtt tattaaagca tcttctccac ttaaatttat aagctgttta
    12961 ggtacttctt gtctactaag tggccagaaa cgagttcctc ctcctccagc cattatgaca
    13021 ttgtatactt tcattattta tctcccctta gctttttata tagaatccac tacatttaat
    13081 atataagata ttaaattgtt caacttaaat tctacatctt cttgattgtc tgaatttgct
    13141 ccaaagtaaa atttaatttt aggttctgta cctgaaggtc ttatagctat ccaagagcca
    13201 tcttcaagta aaaatttaag tacatctgac ttaggtaaat catcaacacc atctttatag
    13261 tctaaaattt tatctacttt tacatcagcc acattatcta ttttaacact tctaaagtaa
    13321 agcatcatct cttttatttt ttttattcca tctatacctt ttaaagttac tgactttaag
    13381 tcctctttgt agtatccaaa tttattatat gtatctatca aagcttcata caagttcata
    13441 ccttttgaag aatagtatgc tgccatttca catatcataa gtgaagatac aacaccatct
    13501 ttatctctag catgagttcc aattaagtaa ccatagcttt cctcatatcc cattataaaa
    13561 cttctatcat tgctttcttc aaacgctttt attttttctc ctataaattt aaatcctgtt
    13621 aaagtattta aacaatctac attataagcc tttgctattt ttgcacctag ttctgatgta
    13681 actatagttt ttattattgt aggattgttt ttaggaagtt tattttcttc aactaaactc
    13741 tctaatatat atctaactaa aagtgaaccg acttgatttc catttaagac tacatattca
    13801 ccacttgaat cttttacaac gatacctact ctatcacaat cagggtctgt tcctattatc
    13861 aaatcagtgt cattattctt tgccatttca atagctatat taaacactga cttttcttca
    13921 ggattaggat actttactgt tgaaaaatta gaatctggta attcttgctc cttaactacc
    13981 attacatttt caaaaccaca ttcatttaag acttttctta ctggtttatt tcctgtacca
    14041 tgaataggtg tatatattat ttttaatttt ttcccgtatt catctattat attttgtctt
    14101 aaaacttgct tcttaacagc ttttataaac tcatcatcta cttcattatc taatatagtt
    14161 attaaatttt tagataaagc ttcttcaaaa tctatacttt taatagtgct gtaatcatca
    14221 atcttattaa cttctgctat tatatcattt gccatatcta tacagatttg accaccatct
    14281 gagtcatata ctttatatcc attgtattct ttaggattat gacttgctgt tattactatt
    14341 cccatagcac actttaaata tctaacagca aaagaaagtt ctggtgtagg ctttaattct
    14401 tcaaaaatat aagcctttac accataagca cttaaagtct ttgctacttc tatacaaaac
    14461 tccctagaca tgtatctact atcatgtgct ataactattc ccttttgttt accttcttct
    14521 ccttgagtct tcatgatata atttaaaacg cccagtgtcg cacgtctaac agtataaata
    14581 ttgattctat tagttcccgc tcctattata cctcttaaac cacctgttcc aaactctaaa
    14641 ttcttataga atctatcttg tatttctttt tcatcatctt ttatgcttaa tagctcattt
    14701 tttgtttgct catcaaaata aggagaattt aaccacatct cataattatt cttataatcc
    14761 atattttcat cctttctgac tctaatttaa aatttcaagg taattatctt cttctaagta
    14821 gtttttgttt tccttttctt aaaaattcaa aagctaagtc catctcttct accttaaata
    14881 aaactatcaa tacagtatat acagaagctc cacctaatac tccagctcca actgatataa
    14941 tttctttaat tgttcctgaa cccataaatg catatagatt attatatatc aataaagtag
    15001 ctactgccat cacaccagat gctaccaaac tttttagacc agtctttata attttatccc
    15061 ctccaaaata tccatttttc tttttaagag atataaataa caatataact gtaattatat
    15121 tggatgtact agttgatata gcaatacctg catgacctaa tggtcttatc aatattaaat
    15181 taagaactat atttattatt aaagctatac ttccatttat cattggcgtt tttgtatctg
    15241 aaagagagta aaatgcccta tataagacat ctcttactcc acaagccaat aaacctagtg
    15301 aatataatct aagcgctata gatgtaagat ctgttgataa cgcatcaaat ttaccccttt
    15361 gaaataatat cctaacaaga ggctctgcta atataatagc tcctatagat ataggaagca
    15421 ccaccaatat aatacaattt gaagatttta caatagtact tataaatcct tctttatcat
    15481 ctttaccaga catttttgct agcttaggat atataactgt tattatagat gtaacaaata
    15541 atgccataac aaactcattt agcctatttg catagttaag agcagacaac tttccatcac
    15601 ccaaagtagt tgccaataat ctgtcaacaa atatatttaa ctgtccaaca gctactccta
    15661 ccaacatagg caatactagg tttactaatt cttttataga ctcatccttt aagtttatat
    15721 atggcttata cttgtaggac ttcttaaatg caaatggcag ttgaaataat agttgactcg
    15781 ccatagccaa tagtgctcct gctggtagta tccatacgtt tttacctgca cttagtgcta
    15841 ttgctgctat tattataata ttatatggga ttcctataag cccaggaatt acaaaactgt
    15901 cattcacctg taaaaacgaa ctaaatatct tacttccact taggaataaa actcctgaaa
    15961 tcattatttt agtaaataat attgctgctt ggaacttagc agggtcgttt ctaaatcctg
    16021 ctgcaaatac tgtaacaaat tgctctgtaa ataaaagccc aataatagct attactatag
    16081 ccattatata gcatatattt aaaacattat tagtaaattt taaggcacct tcatctccga
    16141 gtcttttctt tgtctcaaag tacataggta tataagttgt agcaactgca gagcctatga
    16201 ctgctatgat taaaccaggt atatttaatg ttaatacaaa tacgtctgca tacatacctg
    16261 tcccatagaa atttgcaagt acaagttctc taaggaaacc taatactttt gaaaacatag
    16321 tggctgccat aatccacaat gctgctttag ctgtttttga catcttcttt ctccttatct
    16381 aatttaaact ctaaaagtag aacttttaaa actgcttata aacattattc taattaagta
    16441 aatttaacct atgaaaagct tccttataaa ttaaaaacta tttcgactca tatctaagtg
    16501 ccctaaatgt aatcaatagt aataatagta ttaatactat acaagataag aatgttagat
    16561 taaatatgct cttaaataga ctataatcac ctactgtata cccaacttca actacatcat
    16621 ttatattgct atctttcaag aatttatctt gtgcacgact taagttatca ctacaaatca
    16681 gtattggcat attttctttt gctgatacta ctcccaaaga aatatattcg tttacatcag
    16741 atctgctacc aaagtctaat ttagcagtat agattttctt tatatctttt tcatcataaa
    16801 acttctttat taaatcaaca tttgtacttg acttatttat ttgctttatt ccttctatat
    16861 ttttcaagtt agaatcaatc tctgcaataa attcttttga atcacctaat gcatatattt
    16921 ttttaagatt cttacctttt aagaaatcaa tttgttcctc aaacccttca tctttagccc
    16981 atattattgg cattccactt ttagcggcat aagaatatac agatatggca ttctctaatc
    17041 ctgttgttga gctaagtaaa aatgcttcat caaatcctac tgttctatac atttctctag
    17101 ctatctttaa agatgtatca tatctgtctt ttcctcttat tctttctact tttattccca
    17161 ttttttctat attacgttca gcatctttag atactgcctt taaaccacct actatcgtaa
    17221 ccttctctgg acctagttct tttagataat ttcttgttac tctaccaaga tttttactct
    17281 tagttacaac tattggagca ttttttgcat atgctaaagg tgttgcactt atactatcta
    17341 caattgactc ttcatttaca agaattactt ctttagaatc cttaaatgct cttttattta
    17401 attctgtcga aatctctggt atactccctt ctatcaattc tcttttggga tttgcatctg
    17461 caaatacctc tattgtgcta gaaatagaga ttaacaaaac taacgataat atagtagaaa
    17521 tttttttaat cataagttct cattcctttt cttttttgta ttttttgaca ataaaaaagc
    17581 tctttttaaa gagctttttc attgattagt aatctaatat tttaaaatta tctagctccg
    17641 tctcctgtca atactacttt aacagtttta aagagtatct taaagtcaag taatatactc
    17701 ctatttttta tatagtaaat atcttcttta agtttttctt caggagtgat ttcgtatcca
    17761 ccatttactt gtgcccaacc tgtaagccca ggttttatag cgagcctgtt gataaatcca
    17821 ggtatttcct cattgaattg aacagtaaag ttcggtctct ctggtctagg gcctattaag
    17881 cccatatccc cctttaatat attaaacagc tgtggaatct catcaattct agttttccta
    17941 ataaactttc caaccttagt tattctagga tcatcttttt cagcccattg tgcgccaaat
    18001 ttttctgcgt ctgttctcat agatcttaat ttatatatat agaatcttct cccgcatttt
    18061 cctagtcttt cttgcttata agttattggt cctttgtcct ctattttaat aagaattcca
    18121 aatattgcta ttaaaggtaa acctatcaca agtcctatta tagataaaac taaatccata
    18181 attctttggt acaagtcgaa taaaaccgtt ccttttacta tgctatagtc aatctcactt
    18241 gcaatttctt tatctgcccc tggatagctt ttaattttag gtatttcaca ctcatcatta
    18301 gtatatccag acatagttac ctccatattg gaataaaaat ttttttaatt cttaatttca
    18361 acttatttct atcttatttt tgacttattt ctatattata ctcaataatg agcagattat
    18421 caatacaaga aacatcattg taaacaattt gattctttac aaaaatatat catctcttgt
    18481 tatattttac attataacag gagatgatat gttttgcttt aatatttttt attttatttt
    18541 actgtatctt ctattcctat tccaatttga gttcccattg tttttacttt ttctgcagta
    18601 tttaacttat ccatatcgct cttattgtca ataaatgcac attcaacaag tattgctgga
    18661 taattgttat ttcttaacac atataggtaa tcttttccat tatcaagtgt tcttgtcttg
    18721 attcctctat ttttcatgtt aaatttttct agtattcttt ttaagatatt tgatgctgca
    18781 gtcttagttg ttccaccatt tttatctttt actttgtaat aaatttccac accattacca
    18841 gctccattag acgcattgta atgtatactt gtaaataaat ctggctttat agtgtttgat
    18901 aatgctgttc tttctcctaa agccattgtt ttgtctgtat ctctagtcat aacaacattt
    18961 ataccttttg aacgtaaata ttcagtagtt gctaatgctg tattaagagt atatttcttt
    19021 tcttgtgctc caccatttaa tccacttgtt gcccctgaat cagaaccccc atgacctggg
    19081 tctatcacta cagtttttcc tgctgctgaa cccgaattat ctggttctgt tggtgcattg
    19141 tgttttgata aactagatgc tatagaattt ataacactat ctttcatccc atcaccaatt
    19201 tgatatactg ttcctgctgt tttttctgat aagtttgtgc tatggtatgc agaaacataa
    19261 gtctttggag ttataagtat tggagctttt aatttagctg ctaaaggtcc tgctgttatt
    19321 gagtcaacta ttatgtcaga ttttgcaact aacattgcac tcaactcttt atctggatag
    19381 aaagtcttaa ttacatttgc atttgtttca tgtctatctg ctccagaaac tctatttttt
    19441 gaagtaccat tttttgctat tttagatatt tggtctataa tttttgatga taaactttga
    19501 ctaccaccta tgtaataagc ttcttctaaa ccttgtgaag ataaccaatt atatgtacct
    19561 tgtggtacag agtctttatt agctaatatt ataggttgtt ggtcttcacc agcttttgat
    19621 gaaatattaa gagcatcata ttcaccagca tatccatttg ctatgtatat tttattaaca
    19681 tcatgatact tatctatttc ttttgctata agtaaagatg tttcatgtct atcaactcca
    19741 ccaatacgag ttacatttac atttacagca gatttaattt gagatactgc cttttgtgaa
    19801 actgaaccat catcacctat aattattaca tcacttggat ttagacgttt taattctgct
    19861 tttgtacttt ctggtatatc atctttattt gcaagtaata ttggtgcatc atatgtagat
    19921 gcaagtggag tagctgtaat tccatctgct gctaattctc cattaactat tacaacctta
    19981 ctagacccat ctttccaacc ttgtttactt atctcaacag cagtttcaaa tctagtagct
    20041 ccagcaagtt tttttactgg cgcagaagtt tttctcatat attcatttac tgcttttgtt
    20101 gaatttgcat cacttattat aaactcttgt gtaacatcat aatccttaag atttaatccc
    20161 atagactcta agtcttcttt gtttaaaact ttctgttcat ctttaccatt tatttttatg
    20221 ctttgaagtt cccatacatt taagtattct gggtctttgt tatagtttat tacaccttca
    20281 tatgaatctg tcttttcatt gtaatttaaa gtagcttcaa gtggcatatc ataacacata
    20341 aaatttaatg ttattgaatc agcatttaat ttttctttaa attttacact tgcattagat
    20401 ttacttccta acactacact ttttctttgt aaatttatat taatatctga attagattct
    20461 gtatttgcag tagtattttc agccgctaaa gctacgtttg gtgccaccat tgctacagac
    20521 aacattcctg ttgccaataa ttttgttgtt tttttcatca tatcccccct aattttttgg
    20581 ttctttaaat ctttaaatct tttttaatac atcttaagct tgttttatat tacataaatt
    20641 caatattatg taaatctttt ttcattatag cacaaaaaat tacaaattgt aaactttttg
    20701 taatctttta tggcatggtt tttaaatttt atcgaggaga aattttgatt atatttgata
    20761 gtttccgtat atttgaacaa atcaaagact ttatcatcta aaaatattgc actaaatttt
    20821 aataaatatt tttttcttgt acattataga cattactata acccagaata ggacatacaa
    20881 taaagaaaat ataagagaat cccaagttgt acctgcccaa ggtgttataa gttcatatgt
    20941 agtccactct accaaggtag ttggataatc gactttgttc gtaagttttg gaacattcca
    21001 aaaaacatga ctcaatatct caaggcacat atatgtaaaa atagggctac ttccaagtgc
    21061 tattattggg gtaaatattt tacttttatt tttgatgtca catataaaat aaaatattga
    21121 cagtaacata ccataactgc cagccattaa aagtacaaat gagctagacc ataatctttt
    21181 attaaatgga aaatactgat taaagataaa agcacctatt agcaggatta tgctcataac
    21241 cagtatttta aagaattttt tatattctcc tatgttttct tttaaaagta cacatcccat
    21301 agtgcatccc aacatacctg aagaaattgc tactatacta gttaaaaaac catctggttc
    21361 aaattctggc ttgtacaaat gtcccttgaa gaaatgtaaa tccactaact gtgcaagaga
    21421 accttctagc tcaaatccat aaggttttgc taatatgtag tacccaacta ttataaatgt
    21481 agctatacat agaaaagtaa ttattgtagc agtacttcca acatttaatt tttttagtag
    21541 taaatatact aaactagtca caaaatagac taatcccata cgttgaagta ctccaagtat
    21601 tctcacagaa tttaaatcag gatttcctag gtaatttaag aaaaatccaa ataatatcaa
    21661 taagatactt ctcttgaata tactaagtat tatactcagt gtggatttat tattttttag
    21721 tttactattt attgatattg gaattgtaac acctaaagat ataacaaaaa aaggaaatgc
    21781 aaaatccgca agtgttacac catgccatac agcatgtctt aattgaggat acattctcat
    21841 ccaagtccca ggattattgc agactatcat gagggctatt gatagccctc ttattatatc
    21901 tattgattta atcctactat tcgtaagttt agaatctaca acattatttg aaatt
//
LOCUS       DAMJVX010000049        21809 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMJVX010000049 DAMJVX010000000
VERSION     DAMJVX010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21809
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.051"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            63..938
                     /locus_tag="PC407_001703"
     CDS             63..938
                     /locus_tag="PC407_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDF2497153.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIKEYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            1299..2597
                     /locus_tag="PC407_001704"
     CDS             1299..2597
                     /locus_tag="PC407_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HDF2497154.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSTERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKLLSSDLNN"
     gene            2787..4178
                     /locus_tag="PC407_001705"
     CDS             2787..4178
                     /locus_tag="PC407_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HDF2497155.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            4355..4942
                     /locus_tag="PC407_001706"
     CDS             4355..4942
                     /locus_tag="PC407_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2497156.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            4975..5766
                     /locus_tag="PC407_001707"
     CDS             4975..5766
                     /locus_tag="PC407_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2497157.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(5947..8364)
                     /locus_tag="PC407_001708"
     CDS             complement(5947..8364)
                     /locus_tag="PC407_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2497158.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNLSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKTISLQGNILSIIFIPLGAI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKQAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     SYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLIRNIEKKSL
                     TERLRSQ"
     gene            complement(8377..9042)
                     /locus_tag="PC407_001709"
     CDS             complement(8377..9042)
                     /locus_tag="PC407_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2497159.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGSLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            complement(9124..10155)
                     /locus_tag="PC407_001710"
     CDS             complement(9124..10155)
                     /locus_tag="PC407_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2497160.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFNISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDLSHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            complement(10145..10831)
                     /locus_tag="PC407_001711"
     CDS             complement(10145..10831)
                     /locus_tag="PC407_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2497161.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINLFKQKVKAFLNHLTTKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            complement(10989..12053)
                     /locus_tag="PC407_001712"
     CDS             complement(10989..12053)
                     /locus_tag="PC407_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HDF2497162.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            12315..12986
                     /locus_tag="PC407_001713"
     CDS             12315..12986
                     /locus_tag="PC407_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2497163.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKACLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            12988..13896
                     /locus_tag="PC407_001714"
     CDS             12988..13896
                     /locus_tag="PC407_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2497164.1"
                     /translation="MFILAFIICTVVIFIVSTKINQRRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKSEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            13973..14659
                     /locus_tag="PC407_001715"
     CDS             13973..14659
                     /locus_tag="PC407_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2497165.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            14656..17211
                     /locus_tag="PC407_001716"
     CDS             14656..17211
                     /locus_tag="PC407_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2497166.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYIVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETAMEGFVNDYLQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKRGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTGEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKS"
     gene            complement(17405..18604)
                     /locus_tag="PC407_001717"
     CDS             complement(17405..18604)
                     /locus_tag="PC407_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HDF2497167.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            18723..19610
                     /locus_tag="PC407_001718"
     CDS             18723..19610
                     /locus_tag="PC407_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2497168.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            19800..20357
                     /locus_tag="PC407_001719"
     CDS             19800..20357
                     /locus_tag="PC407_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2497169.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(20597..21457)
                     /locus_tag="PC407_001720"
     CDS             complement(20597..21457)
                     /locus_tag="PC407_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2497170.1"
                     /translation="MKKQKSPILLEMLGLFLGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
BASE COUNT         8156 a         2709 c         3168 g         7776 t
ORIGIN      
        1 tgattataaa gtttatatta aatttatttt attgatgaaa ctaatgggga ggttaaaact
       61 caatgattac ttttgatgta aatggagcta ttatcagatt tgatgataca aaaaataatt
      121 ataataagat aaggaaaatc tttaaattat atgctttaga aatatctaaa aactttgaga
      181 gagattgttt aaatagtttt caaaatctaa agcaaatatc tgatagaggt ttaagtttag
      241 gagaaaaata tatagaagag tcttttaaga aaggaattga gactatagta agttttggaa
      301 ttataactat agatatcgat acctttaaaa gcgtttattg tgaaaaatat ttagatttta
      361 agagactttt taataacttg aataaaactt ttattatacc aaataaaaat aaaaaaaatg
      421 ggcacgctag cttcttagaa atagaatcga caataaagaa aatgagcaat tatttataca
      481 atgattgttt taaaattcat tatgctgttg tagatgccct tatagaaaat cgtataactg
      541 cggtacattc ttatattgat gaagaatcta taaaaaaatc taatgcctta tttaataatt
      601 ataaagatgg ttttattggg aaggctgacg aatgcaaagt agtaaaacaa ataataactt
      661 taaatccata tagggaagat gtatacgaat ttttggtaaa agaagatgga gattttagta
      721 aggaaattga gagattgact gaatttttag gctatgatat aaaagaatat aaagcgtcat
      781 taatggatat ttatataaat gagttaatgg agagcgatga aaatgatgtt gaaaatgcaa
      841 aagaaaagat tgaaaaatat gctagatata ttggatgtag tgatgagaat ttatatgtag
      901 caagagttaa tgccatatat acttttgcaa atgcataatt ttattaaaca ttaaatatta
      961 gatgttcaat tatataaatt taaataaaaa aatatcctta ttaataacaa atagtttata
     1021 ttaaaaacca ttcttaaaat gagtggtttt ttattttgaa tagatatatt ttaaacctaa
     1081 tagcattcct atctttaatt agccaaaaat caactggatt tcatatagac tacataattt
     1141 taaattacta aaaaagacaa tatattgaca aaaatgaaat atgttgataa aatgaaatag
     1201 tcaatatggt gtcttaattt aaaaatttat ttatgttgta ttagataaag ttgttttata
     1261 tataaagcat aaataattat tataaggaga aatagattat gaaagtaaat gtatacaatg
     1321 tagaatcatt tgtggatgaa agacttaaga ttatggtttc agacttaatt ccaaatacta
     1381 aacttaaaat aagtgtgaaa atggaatttc catggtgcaa gggagaagaa ttttcatctt
     1441 atggagtgtt ttgttcaaat gaaaaaggag aagtagattt agatttagtt gaacctatag
     1501 aaggtactta taaggctgat aattgtatgg ggttaattta ttcgctggaa aaatctaaaa
     1561 ctgaaggtaa aaattttgca gaaaatatat ctattgataa accaataatt atgaatatga
     1621 tatttgaaac ttctactgaa agaaaagaag ttgaattaaa gagaatattt aaatcagaag
     1681 atattaccac aaaatacata tctgatgagt ttataggaaa attattttat aaacaaaatt
     1741 ctaataataa aacaatttta atgctaggtg gttctgatgg aaatttagac gctctagacc
     1801 ttttagcagc tccacttgca tctagaggat tcaatgtact aacagttgca tactttgctt
     1861 tagaagggtt accagataaa ttagaagagg ttccattaga atattttgaa aaagtatttg
     1921 agtggattag tgaaaatgaa attaccagta ctaaagaaat atttgtacat gggacatcaa
     1981 agggtggaga attggccttt ttacttgctt caagatatga acaaattaaa aaggttgtag
     2041 tatcacaacc tcacatttat tgtttccaag ctcttaatgg attgatgagt ggaaatgata
     2101 cttcatcatg gtcatataaa ggagagccat ttccatatat caaagtagac aatgatatat
     2161 tctttgaaga acagaaaaaa aatatatcaa aaggtattcc atttggattt aatagtactt
     2221 ataagaaaag tcttaaaaga gcagagaata gaaaagaggc tagaataaaa atagaaaatt
     2281 ccaaagcaga tatacttatg attgctggaa aagaagataa catatggaat agttatgaag
     2341 cttgtttaga ggcattagaa atctttgaag aaaataatta tccatataac gtagaattat
     2401 taacatacaa taatatgggt catccgttac caattccata tattatgcct ctaaaggaga
     2461 ctttatgtat gagtatgagt ggagggattt tttcttcagg tggaactgta gaaggaaatt
     2521 caaaaggaca gtttgaatca tggaatagaa ctattgaatt ttataaaaaa cttttatcaa
     2581 gtgatttaaa taactaatat taagtgattt aagtaactaa aaatgaagcg gtttttctat
     2641 atatgcacaa aatctatcaa ttttatagat ttaatttatt gtttatataa tttgcatgtt
     2701 atatactaat tttaatgaca ttgacaattt ataaacaatt ataaagttgt aaacttataa
     2761 gagttattga aaggtggagt gaagttatgg aaagactcgc tagaagggaa caacctcata
     2821 taaaatttgg aatgtttaag ataagaattc catttataca ttacagattt gagaaaccag
     2881 aagctattca aggtataata tcaagtatga caagtttagg cacaattggc cttagtacac
     2941 aaattttggg attaccctat gaagtggcat ggagtatggc tattattaac tcaatattat
     3001 actgtttgca tgtttttatg ggagacccag ttgtacctgg ctggattaca gcttctataa
     3061 ctcttacaac tgcatatttg ttaaagttca gcatgggcat tgaaagaata caagcattaa
     3121 cagctttaca aatagattta ggaataatat ttattttaat gggtataact ggtgtagctg
     3181 gaaagttagt tagtaagata ccaaattcta ttaaaggtgg aatactaatg ggtgtcggta
     3241 tatcgactat aatatcagag tttaatccta aagggagatt tgattcttat cctatatcta
     3301 taacagtagg aattttggtt gcttgttttg taatgttttc tgaaagattt gatactctaa
     3361 aagttaagaa taaattttta tttcatttag gagaatatgg tgtagtatca gctattttag
     3421 tgagtttaat agtaggtata tttagtaaag aaatagaaat tcctagattt agttttgaca
     3481 atttagttta catagtagat tttaaaaatc tgataaatac agtatctcct tttggaatag
     3541 gttttccaag tgtaatgtta tttatacaag ggataccaat ggcttttatg atatacataa
     3601 ttgcctttgg tgattttata acaggtgaaa acttagtact ttctgaaagt gaaaatagaa
     3661 aagatgaata tatagatttc aattcaaata ggtctaatgt tataagtggg ataagaaata
     3721 tttttatggc aatcttttct ccatatatac caatgtgcgg tccattagct gcgacactta
     3781 caggaagtgt tgctcaaagg tacaaggttg gaaaggaagc catgcagtct gtttttagtg
     3841 gcatgggtac attagtatgg gtttcagcaa tttttatctg tttttatcca attgcgcaga
     3901 ttgctacacc tcttatacca ttagcattgt cagtaacact tttagttcaa ggatatttat
     3961 gtacaaagct atcaatggag ctatgtaata caggtgttga gaagggattg attggactta
     4021 tgggaggtgt aattgctgct aaaggcggaa catggggact tgctgtaggt tttatactgt
     4081 atttcatttt aattgattct aaaaaaagaa aagaaaaaga tattaattca atagaagatt
     4141 gtgtatatga aatattagac aaaaagaaga gtatttaaag gaaaatatga gttgctgtta
     4201 tctattaata gattaattgc aactcatgtt ttatttataa gttttaggaa ttgcaaaaaa
     4261 atgcaaagct ttttcttgac ataatttctt gatatgttac tatagttata taacatatgt
     4321 gttttataaa aatttaatgg agaggtgaac atatatgcca cctaaagtaa agataacaaa
     4381 agaaatgatt ttgaatacag tcttggatat tacaagagaa gtgggatttg aagctgtaaa
     4441 tgcgaggagt attgcaagta aattacagtg ttccactcga ccaattttta cttgttacga
     4501 aaacatgaat gaattaaaaa atgaatttct tgcttttgca tatgaatatt ataatcagta
     4561 tgttgttaat tatcgtaatt ctaaaagtgt aagcccttat ttactgcttc cgctttcata
     4621 cattgaattt gctcaagaag aaacgtattt atttaagttg ttatttataa atgatttaga
     4681 tttgatgatg aaagaggcga atgattttta taaagaaatt gacaatgaaa aaaaggcaag
     4741 acttttttcg gaaagcattg gaatagagtt ggaatgtgca aaagtaatat ttttagattt
     4801 atttctttat actcatggca tagctgtctt aacagcaaca aaaaaattaa cattagatag
     4861 atatagtgct gaaaaaatgt tgaaaaatat attaacagct tttataagac aagaaaaacc
     4921 agattggaat ttgtctattt gatgtttatt aactaatata gaagaggtga gattatggaa
     4981 acaaaacaat acttaattga cttttataat acttatgatg aagatagtcg acttgcatta
     5041 aaacatggaa tggtggaatt tctcactact atgcattaca ttgataagta cataaaatca
     5101 ggtgattgtg tgcttgaaat tggcgctgca actggtcgtt attctcacac actggcacgt
     5161 cagggatatg atgttgacgc agtggaatta gtcgaacata atatagaggt tttccataaa
     5221 aatactcaat caaatgaaaa tataagcatt actcagggta atgctatgga tttatctgtt
     5281 ttcccagata ataaatatga tattacgtta ctgttaggac cactatatca tctttataac
     5341 aaagaagata aacaacaagc tttacatgag gcaattcgtg taacaaaacc tggtggtgtg
     5401 gtttttgctg catacgttat atctgatggc tgtctcattg acgaggggtt ccatagagga
     5461 aatattaatg tatctgaata tattgaaaaa ggtttgattg atccacagac atttgctgct
     5521 aagtctgaac caaaggattt gtttgagctt gtacgtaaag aaaacattga tgatttgatg
     5581 tctgcattta atgtaactag actgcattat gtcgcatcag atggtttggc tttgtatatg
     5641 cgtgaagcag tagactctat ggatgatgat gcatttgcgt tatatttaaa atatcacctt
     5701 gctacttgtg aacgtgaaga tttagtagga gttacaagtc atgcaattga catatttaga
     5761 aaataaggaa tatgcttgtt tatgtctctg gtgatgttgc ttggagatat aaggaagtgt
     5821 atctgtataa aataaaaaca agtagaggga cagaaaatta tgtaaagtca aaattttcta
     5881 tccttttagc ttttaaaaaa ttatgttaaa acttgtataa aaaatggtta ttttttatat
     5941 acattcttac tgagaacgta agcgttctgt aagtgatttt ttttctatat ttcttataag
     6001 taattcagtc actaaaactt gtataccaat taatacgaat gaaaaaatta aaatagtaaa
     6061 tatagaaaat ttaaaattaa taaagtctaa tctaattaca tcttttataa aagtgcaaag
     6121 tagatttgaa ccaaaataac ctagtatact tgaagtacat atagctttga tacttatgtt
     6181 tagaccttct ctataaagca tatttcgcag ttgctttctt gtcattccaa cggcttgaag
     6241 taaggcaaac tctttttttc taattaaaac gctgtttatt agggtattgg tcatatttag
     6301 tataccaaag caagaaatta aaccaacaaa aatgtatgaa gctataatta tatattgaaa
     6361 tgctctagta atcattctat aatcatcttc atatgaataa agcattaaat ttgaattttt
     6421 ctgaattata cttaacaact tttcttcaac ttctttagaa tgctttttat ctattaaaat
     6481 agaaaaatct aaatttgtat tatatggtgt tagagattct aaagttttgt ctgaagcaaa
     6541 aaacatacct cctgtattgg aattgtcaat gattcctata attttcattt tctttttaat
     6601 aatatcattt ccactaaaga tattaaatgt aactgtatct ccaactttta aatttaatcc
     6661 ataagatttt gttctatatt tatttataat taaactatct gaatctaaat gtgtatagtc
     6721 tatttttcca ctgacaatat attcttcaag tgattttgaa agttcaggcg taataccttt
     6781 aattagagta ataaattgtt tttcgtctct atctttgatg taaggttcta taatattact
     6841 tttaattgat ttggattcaa ttaatttttt tactccatga atagatttaa tttcattctt
     6901 aagagaatct gttagtggat tattttgttg gatttctgta atatgctcat ataagttatc
     6961 actcttaatg ctaattatat aatcttcgtt atagacaaat tgttgagata gttgtttttt
     7021 gaaatctatt gaattagcaa gattagcgat tgaaataaat aaaactccac taatgcttag
     7081 cgatattaat gcaatcaaat ttttctttct atttgactcg atattcttta atgctagatt
     7141 ttttatattt atataactaa aattttttgc ttgttttcta aatttagttt ctgtatattg
     7201 catggcttca atagcagata ttttagaaat aattctagct ggtttaaata aagaaattct
     7261 aacagttaaa aacattacca gtgataaaat taaaattata aataaatcag ctatcatttg
     7321 ccatgctaat ggttgaatta tgtaagcaat tatacaagaa gctatagccc ctaatggaat
     7381 aaaaataatt gacaaaatat ttccttgtag agaaatagtc ttttttaact gaaaagaagt
     7441 catacctaac gatttcaact tagcatacat ctgaactgaa ttaacaactg aaatataaaa
     7501 aatactatat ataacaaaaa cgctagaaaa aagaacaact attccaacta ctaaaactgt
     7561 aataatctgc tcaccatcaa ttaaatttga ttcaatatag ttatcattta agtatatatt
     7621 atatttctta atacctaaag ataaaccaat tttctttaac tgtgtcttaa ttgaagctga
     7681 atctttctct gtattttctt ctgatagttt aactaataca tctacagtat caggtcttga
     7741 ctcgtcaaaa gtattacttt tattttgtaa aggtgtagaa gctatattct taaaataatc
     7801 atttgaaacg actgtatagt attgcttggc attatcttga taataatttt ctagaaatcc
     7861 aacaataata aaatcacatt gaacttcttt attcgtaata tcatcataat aatttaattt
     7921 aatgctatct ccaatatctt tatctaaacc aaattcctgt aaatattttt ctgaaatagc
     7981 aatttcattt ttatttgtag gtagtttgcc tttcaatggt tgaaaattca taagtttaat
     8041 tgctgtatca tctgcataaa caatcgacat aatacagtct gtctttttat tacttccaat
     8101 taatttgtag gttcctacgc cactaaggtt tttatcttta tatagtatat tttttgcttt
     8161 ttcatctaca gatttatata tagcatgata tggagatgca ttaactaaat ctacagaagc
     8221 attatatgaa actaaggtca ctgttaataa taaacatgta gaaatcatta taatgatacc
     8281 tgaaagatat gttttaagtt tattaatttt taaatcattt aaagctattt ttcttataaa
     8341 tgtgcgatta ttatttctaa acatatacta cacctcctag ttaacaattt ttccatcttc
     8401 aatacgaata attctatcag ttagttgtgc aatttcatta ttgtgtgtaa tcattacaat
     8461 tgtttggttg aattttttac ttgtcacttt aagtaaactc agtacatctg aactagttct
     8521 actatcaagg ctacctgtgg gttcatcagc tagtataata gctggttttg ttacaagtgc
     8581 tctagctata gcaacacgtt gctgttgacc accagaaaga ttacttggca tatcatataa
     8641 tttttcttca agagaaagca tatttataat ttcatttata aaatccttat ctatggtctc
     8701 tccatccaat cctattggta agataatatt ttcatataca ttaagtaaag gaacaagatt
     8761 ataattttga aatataaaac ctatatttct gcgtctaaag attgtaagct gttcatcagt
     8821 catagtagat aattccttac cctttataat aacctttcca gatgtaggat tatctaatcc
     8881 tcccatcata tttaataatg tggatttgcc actaccagat gtacctacaa tagaaataaa
     8941 ttcgcctttt tcaatagata gagttacacc atcaagagct tttgtaatgt ttgaactttg
     9001 tccataatat ttctttaaat taattacttg taaaatactc ataataatac ctccttaaca
     9061 tcttgtaaat acagtataaa atcaaaatgt tgcagaatca ttacaaataa aatatttaaa
     9121 aatttattta ttaggtaaga agatagaaaa agtcgaccct tttccaatat ctgatgtaac
     9181 tttaatatag ccaccttgaa gagatataat ttttcttgaa agatatagac ctattccaat
     9241 accttctatg tcatgtacat cttcttcacg ataaaatctt ttaaagattt ctgcttgatg
     9301 actttctgat attccttttc cattatcaat aatatctatt tttgtataca taacccaaca
     9361 ttctgtagct acacgtatct taccattaga atttgtgtat tttacagcat tatctaatat
     9421 attaaaaata gcttcagaag tccatttctt gtcatgaatt atatataaat ttgggtcaca
     9481 atcaactgta acttcaatat tctttttttc agcatcaaac aaaattccac taagtgctgt
     9541 agcaattgtt tcataaattg aatttttctt tttagatagt gtgattatac ctgtctctag
     9601 tctggaggtt tttaccatag attgcattaa aaaatctaat ttatctaatt ggttttccat
     9661 atccattaaa aaatcctttt gaatttctgt tttaaatgtt tgatttagta gagtagtatt
     9721 aatcattttt aaatttgcaa taggagtttt aatttgatga gatagatcag aaactagttc
     9781 ttgtaaatca gctttctcta tagcaatact attgcgattt tcttgaataa cttcatacat
     9841 tctaattaat ctgtgattga tacgtgataa aatagtttct tcttctaatt caatttcaat
     9901 attgtcttca ctattcatca tactatctaa agtgtgacat attttagaac aaaatataac
     9961 tattttttta tgaagtaaaa atattattaa tgctataaat ccacaaagta ctattgtata
    10021 tattaataat agagcaatta gttttaaatc atcagttatt agataaattg aaatattaaa
    10081 taaaatagct gtaaaaatta taccaacagc aattattaaa taaatagaac taaaagaaaa
    10141 ttttttaaaa ttcatttctt tcacctaacc acatatatcc cataccatat accgttttga
    10201 tatacttaag attgtctttt tcaattttgc ttctaatcct acttataaca gatgttaaag
    10261 tatgttcatc tacaaagtta gcatctatat cccagagttt ttctaaaagt actttccttg
    10321 ttaaaatact tttaggattt tcagtaagca gttttaaagt acgatattct aatggtgtaa
    10381 atataatttg atttccattt atattggcag ataactcaga aaagttaatt tcaagatatc
    10441 catccctata gtaatcttgt tttgtttttg ttgtaagatg atttaaaaaa gctttaactt
    10501 tctgtttaaa taaattgata ttaaatggtt ttgttatata atccaaagct ccaagttcat
    10561 agcctttaat catatcacat tccatatcat tagcagttaa aaaaattatt ggtatattgt
    10621 aattttcctt tatttcatta cataattcaa acccactttt atctggtaaa ttaatatcca
    10681 aaattattaa atcaaattca cattcaaaaa tagactttag tgcagattta gctgtaaatt
    10741 ttgaagtaat tatatatcca tcttcaatta ggttatatga tagagtttta tttaatatag
    10801 aatcatcttc tactactaat attttttcca tacagttact ccttttagtt taatatttta
    10861 taatgtcaat tatagactaa aaacattgct gaattgttat aaacgagaaa aagtatttaa
    10921 aaatttatat atttaataaa gaacctgtcc tatgataaat tctagtacag attctttatt
    10981 aaataaaatt atttcaacca agatggattt ccaggtataa atattctttt accaaatttt
    11041 gaagtatact catctaaagc tttttgaata agctcattat tacccttaaa tgaagtagta
    11101 ttctttatag tttcgagatt ttggctaaat gggcaagcag atatacacat accacaatct
    11161 gtacctaaat atctccattt aatatagcat gtctcatgtt caattaccca attatatttt
    11221 ccatcatcac caattttaat atcattagaa agcgaatgag aagggcagtt aaaagagcat
    11281 tttttacaga ccttacaaaa atcttctagt ccaaaatcaa catattcatc ttctaaaaga
    11341 ggaatatctg tagtaacaac acctaatcta agtctagagc cataatcttt atttgtaagt
    11401 atagcatttc ttcctatatc tcctaaacct gcatcacgag ctataaaaac aggcattact
    11461 aaataattag catccatatg atttctagca tcatatccta aatttcttat atagtaactt
    11521 aaaatcatgc cgacgataga tgcatccaca tagcactttg atgtttctat aacttcacat
    11581 atcattggag ctctatttat catttcttta tccatctcac atccaaatac aattgcatac
    11641 ttatgagtta gattaatttc ttcaccgtaa ttttcttcat gtctacccct atttgtataa
    11701 atatgatagt cttttaattt agttatacca actacagaag ctccatactg ctttgcaaga
    11761 cccttaattt tttttgtcat tgactttgca tctacatcta ctttagtatc agaaactttt
    11821 ccttcgcata aagatttgat atcagataaa aaatcgaatg cactacttgc cattggagag
    11881 tttaactcat tgtaagtcat tgttccttca ctacataaat ttggtctatt tcttatacta
    11941 tcatcaattt cttttttgtc aggatttttc ttataataat cattataagc agtactatct
    12001 tttttgtaat ttgctcttgc aaacatagta tttctctcat caaccctttt caattctatt
    12061 ccccctagta aaaatatatt taaaaaatat aactttattg ttgtataatt taattatata
    12121 agaataacgt tatattttca atttaagcgt aattaaattg ggaaatatat aactatatgc
    12181 ttgattatga tgtttttatt ttgaaaacaa atttaaatac aatcttttta gtgaaataaa
    12241 taaaattaaa atatttttag tatgataatg caagaaaata attatttatc taaaaaatct
    12301 aaaggagagt aaaattgaac ttattaatta ttgaagatga tataaactta aatgaaggtc
    12361 ttttttatgc atttgagaat gatggattta atgtatttaa ggcttataca aaacaagaag
    12421 gattaaatat atttaatagt aaaaatatag attttataat cttagactgc aatcttccag
    12481 atggagatgg atttgatgta tgtcaaataa taagggaaaa gtcagatata ccaattataa
    12541 tgcttactgc aagagactca gaaattgatg aagtaaaagg attagaaata gggcttgatg
    12601 attatattac aaaaccattt tctttatctg tattaaaggc aagagtcaaa gtagcaataa
    12661 gaaaaaaatc gaataataaa gtaatatatt ccaatggaat aaaattggac caaaagttac
    12721 taaaagtata taaaaataaa gcatgtttgg aacttagctc tgtagaatat aagcttctaa
    12781 gttatctaat tgaaaataaa ggtcagatat tattaaagga acaaatttta caccatattt
    12841 gggacagcga agaaaattat gttgatgata atattgtatc tgtaaatata agaagactta
    12901 gagtaaaagt agaggatgac ccatctaatc caaaatatat aaaaacagct tatggaatgg
    12961 gttatttatg gaatgaggta gaataatatg tttatattag cctttataat atgtacagtt
    13021 gttatattta tagtatctac taaaataaat caaagaagat atgatgaatt aaatttgatg
    13081 ttaaatcaaa tattggaagg taaagaagtt acttatccag atacgaagga tacaagagcg
    13141 tcaaaaattt ctcaccaagt aaaaaaaatt aaggatatga tagaaataga ggtagaacag
    13201 tcaaagtcag aaaaagaagc gattaaaggt ttaatatcaa atatgtcaca tcaattaaaa
    13261 actccacttt caaatataac catttattgt gaacttctgg agaatataaa tatttccaca
    13321 ttacagaaga aggagttttt acaaaagatg aaaaatgaaa cttttaagat tgactggttg
    13381 ttacaatcct tatttaaaat gactaaatta gaggatggag ttatagaatt tgaagtagaa
    13441 gaactactaa ttaaagatac tcttattcaa agcatcagta ctatctttaa taaatcagaa
    13501 gctaaaaata taaggataaa tctcgaacca ttttcagata taaagttagt tcataataaa
    13561 aagtggacca tagaagcaat tgtaaatgtt ttagaaaatg caataaaata ctcaccatca
    13621 gatagcacga ttactatttc tgtaataaaa atggagctat atacgaagat tactattaaa
    13681 gatgaaggaa taggtattga tagtagagaa ctaaatgata tttttaaaag attttacaga
    13741 agtaaaaatg tggaaaacca aaatggcaca gggattggtc tatatcttac aagattaatt
    13801 ttagaaaaag aaaatggaaa cattatagta gaatctaaac taggaagtgg atgttgtttt
    13861 agtatattct tacagaattg taagagttta aactaacttt gtaggatgag tgtaattatc
    13921 gtcacttaca atattttata gaacatatat ctgtattgaa aggagaaatt aaatgagtat
    13981 attagtgacg aataatttag tgaaacacta tggaaaaggt gagactaaag taaaagcctt
    14041 aaatggagta agtatagaaa ttgaacgaga tacatttact gctatagttg gaacttctgg
    14101 ttctggaaaa agcacattgt taaatattat aggtggatta gataatccaa cctcaggtga
    14161 tgtaataatt aaaggaaaga acatatctaa aataggaaaa aaagatttga cagtatttag
    14221 aagaagaaat ataggattta tatttcaaaa ctacagttta atgcccgtat taaacactta
    14281 tgataatata gctcttcctg ttacattaga taaaggaaac catatagacc atgaatatat
    14341 tgaaatgctg atgaagacat tgggaatttg ggataagcgt ttaaaatttc caagcgaact
    14401 atctggtggt caacaacaga gggtagcaat tgctagagct ttggcaaata aacctgcact
    14461 tatattagca gatgaaccaa caggaaatct cgatagtaaa actacaatgg aagtggtatg
    14521 tttattaaaa gaaagtagtg caaaattcca tcaaacgata cttatggtaa cacacaatga
    14581 aaatatagca caagtatgcg actcaattat ccatattgaa gatggagttg tggtgaatac
    14641 tggtggtgaa gtactatgat gtctctagta aaattttcaa ttcaatatat aaagcattat
    14701 aaaaaacaat ctatatctat agttttaagc atagtactat cagtagcatt attgacggga
    14761 attggttctt tagttcatag tgcagacaaa agtagaattg aaaagataag ggaagatagt
    14821 ggagattatc attatttcta taaagtagat aaggaacaac ttaataaaat taaaaaaaat
    14881 aaaaaatcaa aggaatacat tgtagataga cttggcgtta caaaacctaa aaatcagata
    14941 gatgaacctt ttattataga atttttaaat gctgattctt catatttaga catgacaggt
    15001 agaaaattat taaaaggaac tcttcctagt aaagatggag agatagtact tgacacatat
    15061 tctctaaaca atttaaatgt aaacaaagaa attggaacag aaatcaattt agatggtaaa
    15121 atctataagt tatgtggtat tttatcaagt ctttcaaacc cagaaactgc tatggaggga
    15181 tttgtaaacg attatttgca gcctaaagaa aaggattctt atatagttta tgtaaaatat
    15241 gatgaaaata aaaatattaa aaagcaaaat tataaattta tgaaaaaatt caacatatca
    15301 aaagaaaaaa cttatataaa ctggcctctt tcagttgaat ttggagtaaa accaccaaag
    15361 aatatagaaa aatataatat atttgagttc ttaaaaaatt tagaactaaa tacaaatgca
    15421 attatattgc ttgttggagt gtttggtgca gcagcagtat atagtatttt ccatgtttca
    15481 atacttcaga gaatatcaca atatggggtt ttagaagtat taggcgctaa taataaacaa
    15541 cttttatttc tactattttt agagttattt ttacttttta ttgttggatt tcctataggt
    15601 tgcttcttag gtattggagt agcgtctaca atatatgaac aatttccaca tatatttttg
    15661 agtagtgata ttgttccagg agcatttttt ataagtgaaa aatctattta tttaggcttt
    15721 ttatttatct tattgttgtt aattttaatt gctgtacgtg ttgtacatca gttgaataaa
    15781 tactctagta tagaatctat gaaaaacttt aagattacta ataagaaaaa gagaatatta
    15841 gaaagtgtaa aacactctaa catgacaaaa gtattatctc ataaatacat gactcaaaaa
    15901 aggggaatgt ttattggaat tttgttttca ttatcacttg gtggaattat atttctatgt
    15961 tctagttact ctatacagct tactaaaaac aataatgaac ttacaatgaa agctgatgat
    16021 ggtttaaatt ctgactacca aataactatg caaacaacag attttgatgt aggtatacca
    16081 aaagataaaa tttcaagatt aaacaatgtt gagggaatat caaaagtcta tccagtaaga
    16141 tatttatttg gagctatgca aattaaagaa gaacaactat tttggaaaaa tttctttaaa
    16201 cctcttgaaa aagattacag gataaagact ttttttgatg gtatttgtac aaagcaaaat
    16261 gaaggtggct atcttttaaa aacaaatatt tatggttata acaatggtat gttagaagga
    16321 ttaaattctt atattataga tggggagatt aatacccatg atatggttaa aaataataag
    16381 gttatagtta gactacctat ggatggaact gggatgtatg atgcagttga tataaatcca
    16441 ggagatacaa taaaagttaa agtgccaaag acaatgaaac caacaggtga aattgttaaa
    16501 tttaaagaag aaaataagaa tgattataca acaaaagaat ttgtagttgc tgctactgta
    16561 aaacgtgtga tggcaaataa catatatttc attggtgatt atggtatgga tatagttatg
    16621 acgaatgggc aaatgaaaaa taactttgat atcgaaaatt ataattcagt aagtataaaa
    16681 aagacagaag atagtaacag tgaggaattg tctgagaaca taaaaagtgt tgtaagtgat
    16741 gtaaaaagat gtatcgtaac agattatacc attgctattg aaaaaaataa tacctactta
    16801 aatcaaaaac tcttatttat atatggtatt gtatttgtac ttcttgcaat aagtttgttc
    16861 catataatca atacagtaag ctacttaatt ttttcaagaa gacatgagtt tggaatttta
    16921 agagctatgg gtattacgga taataagttt ttacttatga tgataagaga aggtttttta
    16981 tatggattct atgcaagtat tataatggta attggaagtg ttattggaca atttatgatt
    17041 tattttatgg taaaacgtgt atatttatat ataaatccaa ttcttaaaat aaatacacct
    17101 ctatacatag gtatgattat tttaaatatc actattagta tagttgctgt aattattcca
    17161 gtaagacaaa ttttgaagag tgatattatt tcagaaataa ataagagtta gagttatata
    17221 aatttttaag attatataac tttatgacaa tcttgtttaa attatttaaa tatatcttat
    17281 ctgtgaacat taaataaacc tcttaataga tataactctt gaaaagtcat atctattaag
    17341 aggttttaat aaattttcat gttaatatat ataatccaaa aaaattaaat cttattaaaa
    17401 catactattt tgtatagtta tcgaaatatc cttgtatgta aataatagga gtacccttat
    17461 ctccacttcc agatgttaaa tctgctaaag aacctataag gtctgttaat cttcttggag
    17521 tagtaccttg agaaaccatg tttcctgtta aatcatcaga tttattatct ttttcaacta
    17581 tgtattttga aattgcttct tttaactcat caccagataa gtcagcaaaa tcattatctg
    17641 ctaagtattt taattttact tcgtttggag ttccctctag cccttttgta taagcaggag
    17701 atactactgg gtcagctaat tcccatattt tacctacagg gtctttaaaa gctccatctc
    17761 cataaaccat tacttctaca tctttccctg ttatttcttt tatattacct tggattttgt
    17821 ttacaacttc atcgcaattt atagggaata attttactgt ttcttctgta gctttgtttg
    17881 aacctaaaag tccatactgg tcattgtaac cactaccatc tacacttgct gtcattatat
    17941 catcaagtga atatactttt ttagctccat tttgtcttaa aattctcttt gttctttgtc
    18001 ttgtatgtat atcacaagtt aaaatacttg ttgtatagtt aagaatagtt tttggattgt
    18061 ttgcaaatac tatttctacc tctgcaccac aatccttgat taaattctta taataatcaa
    18121 cataatctac accagtaaat gtatgcttgt tatatccaaa tagttctctg tatttttctt
    18181 ctgttaaaac atctgaccaa ggatttattc ccttatcatc aagttcatca atacttatta
    18241 agtgatttcc aacttcatca gaagggtagc ttaacattaa tacgactttt ctacatcctt
    18301 ttgcaattcc ttttaaacat atagcaaaac gatttctact taaaattggg aatataaccc
    18361 caacagtatc atctccaaat ttatctttta catcttttgc aatattgtca acgtgagcat
    18421 aatttccctg tgctctagct acaacagctt ctgtaacggc aattacatct ttgtccctaa
    18481 cttcaaagtt ttcacttttt gacgcattta aaacagaatc aacaactatt ttaactaagt
    18541 catctccttg tcttataata ggtgctctaa cccctcttga aacagttcca actaatctat
    18601 ccattatcta aaatcctcct aaagaattaa tattgtcata ttagtaccat gtatattata
    18661 attcaaatat aattataagt aaaataaata atactaatat ttgacataag gagtaattat
    18721 atatgaatat aaaacttgaa ctttataagg tttttaatgc agttgtaaat aataagagtt
    18781 tttctcttgc tgctaaggaa ttgtttatgt cacagccagc tgtaagccag tcaataaagc
    18841 aattagaaga acaattagat actttattat tttatagaaa caataaggga gtaaaattaa
    18901 ctccagaggg gaaaattcta agtgaacatg tcactactgc attaaagttg atatcatcag
    18961 gagaagatag aataaataaa tttaaaaaat tagaatacgg ttctctgaaa ataggagtag
    19021 gagatacagc tgcacgtttt ttcttgttaa aatatttaga aatttttcat aagaagtatc
    19081 cacatataca tgtatctaca ataaatagaa cgagtagaga acttatatct ttactcaaag
    19141 atgggaatat agatatagca attataaaca tgccaataga agatgataca ctaaatattg
    19201 ttgagtgtat tgaaatacat gatatatttg tatgtgcaaa tgattacata gaatacaaag
    19261 gtaggaaaat atcattggaa gaattaaaca cattgccttt aataatgcta gaaaataaag
    19321 caaattcaag actttatgta aatgaatatt ttttatcaaa aggtataaaa ctaaacccgg
    19381 atatagaatt gggttctcat gaattacttt tagaatttgc gtatataaat cttggagtgt
    19441 cttgtgttat agaagaattt agcatagatt atttggaaaa tgagaaattg tttaaattag
    19501 atataaaaga gccaatacct aagagaaata taggttactg ccatttaaaa gatatatctt
    19561 tatccttagc aaccaaagaa tttttaagca tgatatctaa taacatataa tttagaatac
    19621 atatttcaca agtaatttaa tataaaaata gagataaatt cgtaaatttg aaagattcat
    19681 atagtaatta gtttttatta aaaataaaga taaggttaat ttgagaacat ttatataatt
    19741 aatatcgtat tttaaatatg aacagtatat ttagattaat gtataaaggg ggattagaaa
    19801 tgtctgattc tgaatatcct caaatgaaaa ccttagaaac tagaagttgt atactaagac
    19861 ctgcatcatt agatgatgtt gaagattttt ttgactgtta taaaaataaa atagtagtaa
    19921 aacatttacc atttagtgag cataaatctt tagaagatac aagaaagttt ataaaatcat
    19981 tttttctaaa tccctataaa aaagggaaaa ttggccactt tgctatagta tataaaagag
    20041 acaataaagt tattgggaat atggggttta ataatataaa tcctaaagca ttagaagcag
    20101 aaataggtat atgtataaat ccaagctatt ggggacatga ttttgctact gagataacaa
    20161 aaagagtaat acagtatgga tttagagaac taaacttaaa taaaataatt gcaataacct
    20221 atgaagaaaa ccaaaattct acaaaatcat tagatttatt aggatttaaa tgtacaggaa
    20281 agtatatcaa aaaaattcat actggaaata ctgtaaaaaa tgttccttgt tatcaatatg
    20341 aattgaaaaa acaatagata taatcattta atatctatcc tggttattta gtaatgattg
    20401 ttttctaaat aaaatttcaa taaagtagtt gactcaaaat atagataatc ctgtttttta
    20461 ttgttaaaat actgaaaatg catttgattt tatacaaaaa agagtgtctc atgaactaaa
    20521 aagtttatga gacactcttt ttgaataaat cgtattttaa ttaaaatagt atatatactt
    20581 aaaatattaa tttttattag ttctctaaat ttttaaatcc ttctccaaga acctcatgca
    20641 catctgtaac aataacaaat gcagtagggt cagttctttt aactaatctc tttagataaa
    20701 gttcttgttt tttagaaact acgactaata gaacttcttt attttctcta gtgtatccac
    20761 ctttaccatc aagaatagta agacctctat caagttcttc agttatagct tgtcttaaac
    20821 tttcaggctc atcagagata ataaaaaatg cttttgaatg gtcaaatcct tccattatca
    20881 tatctgctat ttttattaat acacaaagtg ctataccaga gtaaagagca gtttctaaac
    20941 tacgatttac tataccagaa gatataacaa ccattccatc taggcatgac atgaattttg
    21001 tagcttttat tcctgggaat ttcttactaa gtatgagtgc aattaagtct gttcctccag
    21061 tagaagcatc tgatttaaac attattccta aacctatacc aactaggatt cctcctgcaa
    21121 tagaagacaa taataagtca ttagtaaatc ttagttttga taatgggtct gttaattgaa
    21181 ctatagctga aaatacaact gttccaaata aagtttttaa tgaatttttt gtacccataa
    21241 ttctaaaagc aagtattact aatgggacac ctataattaa catgatagct gatactggaa
    21301 gtcctgtaac cttgtttagt actaatgata gaccactaag tcctccagga gcaattgtat
    21361 gtggttttaa aaacatattt ataccaatac tcattgatat acaacccaaa aatagtccaa
    21421 gcatttcaag taaaattggc gacttttgtt ttttcatata aacacctcca tgaacttgtc
    21481 acaatttaat attataacat atttgaccag tttatattat ataaaatttc ttgatataag
    21541 caatttcaag tatttcatta ttcttaatat ttttgaattt ttatatagtg ttttcatatc
    21601 tttactgatg ttgttacact cctatgaagt tttttatata ttataatttt tttattcttt
    21661 tataaagtat cccctataaa gatggttcta tgaaaatttt ctctatactg tttaacgtat
    21721 atagagattt tgtgtataat tataaggtat ttaatggaaa aaattatctt gcttttatat
    21781 gttatcaaaa ggatttatta aatacaaca
//
LOCUS       DAMJVX010000050        21692 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMJVX010000050 DAMJVX010000000
VERSION     DAMJVX010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21692
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.052"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(361..1452)
                     /locus_tag="PC407_001721"
     CDS             complement(361..1452)
                     /locus_tag="PC407_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497171.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEMTVMKEDISEV
                     KQEMTVMKEDMSEVKQEINVLKGDMSEVKQEMTVMKEDMSEVKQEINVLKEDMSEVKQ
                     EMIVMKEDMSEVKQEMTVMKEDMSEVKQEMTVMKEDMSEVRQEINIMKEDISEVRQEM
                     TVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINV
                     MKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIE
                     IKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKED
                     IAEIRRDLNLVELATSKNWSDIVKLKSVK"
     gene            complement(1682..2767)
                     /locus_tag="PC407_001722"
     CDS             complement(1682..2767)
                     /locus_tag="PC407_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2497172.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2803..4116)
                     /locus_tag="PC407_001723"
     CDS             complement(2803..4116)
                     /locus_tag="PC407_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2497173.1"
                     /translation="MIAIKSKYLISSADEIYTNSAAVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(4117..6408)
                     /locus_tag="PC407_001724"
     CDS             complement(4117..6408)
                     /locus_tag="PC407_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2497174.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDTKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(6398..7192)
                     /locus_tag="PC407_001725"
     CDS             complement(6398..7192)
                     /locus_tag="PC407_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2497175.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(7231..7677)
                     /locus_tag="PC407_001726"
     CDS             complement(7231..7677)
                     /locus_tag="PC407_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2497176.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7749..9155)
                     /locus_tag="PC407_001727"
     CDS             complement(7749..9155)
                     /locus_tag="PC407_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2497177.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(9173..10543)
                     /gene="hydA"
                     /locus_tag="PC407_001728"
     CDS             complement(9173..10543)
                     /gene="hydA"
                     /locus_tag="PC407_001728"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2497178.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKN
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10676..11863)
                     /locus_tag="PC407_001729"
     CDS             complement(10676..11863)
                     /locus_tag="PC407_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDF2497179.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSV
                     YGK"
     gene            complement(11990..13204)
                     /gene="dpaL"
                     /locus_tag="PC407_001730"
     CDS             complement(11990..13204)
                     /gene="dpaL"
                     /locus_tag="PC407_001730"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2497180.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            complement(13567..14238)
                     /locus_tag="PC407_001731"
     CDS             complement(13567..14238)
                     /locus_tag="PC407_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2497181.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            complement(14321..16537)
                     /locus_tag="PC407_001732"
     CDS             complement(14321..16537)
                     /locus_tag="PC407_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2497182.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHNIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFRGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLIVEEAEKLLETSKEDLYLDNGYVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGLTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVNSYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            complement(16540..17010)
                     /locus_tag="PC407_001733"
     CDS             complement(16540..17010)
                     /locus_tag="PC407_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HDF2497183.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIKEAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            complement(17072..18466)
                     /locus_tag="PC407_001734"
     CDS             complement(17072..18466)
                     /locus_tag="PC407_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2497184.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQLAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            complement(18539..19900)
                     /locus_tag="PC407_001735"
     CDS             complement(18539..19900)
                     /locus_tag="PC407_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2497185.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            complement(20198..21520)
                     /locus_tag="PC407_001736"
     CDS             complement(20198..21520)
                     /locus_tag="PC407_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2497186.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
BASE COUNT         6447 a         4411 c         2397 g         8437 t
ORIGIN      
        1 caaaatagta atattatttt tatagtaaaa attttattca tattactatt tataattttt
       61 atagagcaat tttagtgcca atatttgcat acatttgtat agtataaaat ttttatgttc
      121 tcattgataa aattttactt atttcaagcg ttttattcga ttttatcatc taaattttag
      181 gttttatttt tataaattaa ttgtatttac ataaaaaaaa agacctaaaa acaaattttt
      241 aacggtcttt tctcaatttt ttattttcat ttctaaaatt tataataaat agtaagaaca
      301 ctataaattc ttttatataa aattcttagt ttgtggatga taaattagtg aattaaaata
      361 ctactttact gattttagtt tgacaatatc actccaattt ttactagtgg ctaactctac
      421 taaatttaag tctcttctta tttcagctat atcctctttt atagaattag tgtcaccttt
      481 tatatatgct atgttatgtt ctatattatc atgagtagct ttatttattt cagaattatg
      541 taataaactt cctaaaatat tattctgttc tatctgtatc tctttcattg atttatcttc
      601 acattctaga ctatcaatct taatttcaat tccatataat ttttcatttg ctatggatac
      661 ctcgtcctta attccattca tatcttcatt aatgttattt attttatttt gcataaagtt
      721 tacttctgat ttaacttcgg aaacatcttc tttcattaca gttatttctt gtcttacttc
      781 tgacatgtct tttttcatta catttatctc ttgttttacc tctgatgtat cttctttcat
      841 tacagtcatt tcttgtctta cttcagacat atcttctttc attatattta tctcttgtct
      901 tacttcggac atatcttctt ttaatacatt tatctcctgt tttacttccg acatatcttc
      961 tttcattaca gtcatttctt gtcttacttc ggatatatcc tctttcatta tatttatctc
     1021 ttgtcttacc tctgacatat cttctttcat tacagtcatt tcctgtttta cttccgacat
     1081 atcttctttc attacagtca tttcctgttt tacttccgac atatcttctt tcattacaat
     1141 catttcctgt tttacttccg acatatcttc ttttaataca tttatttcct gttttacttc
     1201 cgacatatct tctttcatta cagtcatttc ttgttttact tccgacatat ctccttttaa
     1261 tacatttatc tcctgtttta cttccgacat atcttctttc attacagtca tttcttgttt
     1321 tacttctgat atatcttctt tcattacagt catttcttgt tttacttccg acatatcatt
     1381 ttttatgcta actacatctt catttatagt atataataac tctaaaatct tctcttgcac
     1441 ttgcatcacc atccctatta attattataa cattttacac aaatgctata caatgttaac
     1501 atatatatca gaattattaa aattttaatt aaacaaaatt gattcacttt attacttcaa
     1561 aactaattaa tatctaaaat aactacaaat aaacaaaaat aaggtcaaca acaaaaaagg
     1621 cttatctctt tgagatagcc tttcttattt ttatttaaaa ctttgttttt tataaaataa
     1681 tctatacatt cagctctgaa catgaaattt ttcttccaag tgcttttggt ttacaaactg
     1741 atatacaaag tccacaatat ctacactctt cttcattcaa ttccatctta tcattcaatt
     1801 ttctcgcttg atatgcacaa actctctcgc acatcttaca tttagtacat ttttcttcat
     1861 cataagtaaa ctcaaagcta agtctttcat taacatctcc catatgatta tgtactatac
     1921 cacaagcatt ttctatagta ttatatccta atctattaag attagatttt aaatcatcat
     1981 gtattttaga tattacttgt gcgcctttta aaattactac tgaacatata ccaacaaagt
     2041 cagcacctgc cataatcatt tctaaggcat catctccact tacacatcca cctaagccaa
     2101 ttattggtat attaacttgt ttttttattt catatattct ctgtaaagtt attggcttaa
     2161 tagtttctcc acttaaatat cctattccat ttccacctaa taaaggttgc cctgtttcta
     2221 tgtctattct aaatgctggt cctacagagt cacaagcagt tattgcatct gctcctgctt
     2281 cttctaattt tttagcaaaa tcgcctatct catctatcat aggaggaagt ttaactatta
     2341 caggtatatt tactctttcc tttgcatctt taagcattgg tatcaaatct ctatagtcat
     2401 aagatactaa ttcaataaag tcagctcctg catttgcaac tttctctact aattcgctac
     2461 tttcttcaat tgtatgacct actgaagcta tacagacagt cccatctctt ttcatttgtg
     2521 gtatttcaaa atctatccat tgctctggct caaaatctgt ccatttttca ttatttaaaa
     2581 gagcattagc tgtatttcta accatatgtg gtgctggatt tatagctctt tcttttctta
     2641 tagtcttagt cacaactgcc cctgctcctg catcacttaa tatcttgcat ccagctgcac
     2701 tatatgttaa tggtcctgaa cctattataa gtggtgattt taattcttta cctaaaaaat
     2761 tatacattat ctgcttcccc ttttcatata tatttacatt cgttaaaatt tccaaaattc
     2821 acaagcaact tctttcattt ctttaacaat agctttttta tctaaatcaa ctaactgttt
     2881 ttcttttaaa atatgtctac catctatgta tacatttgat ataaattctt ttttaccctg
     2941 aactactatc tggtcaaata tattatcaag agtcacagga gttttaaatt catcttccat
     3001 aactattata tcagccttat taccaactgc tattttacca gaattttgaa gtcctaatac
     3061 ataagcacca tgctctgttg ccatcctaaa tactaaattt gctggcatta ctgatgcatc
     3121 ttcttcaact gatttatgca ttaaaaatgc acctctcata acttcaaaaa aatcatttat
     3181 atatccatca gtacccagtg ccacttttat gcctttttta atcatttttg gtactggaga
     3241 aaaacctcca cctacttcac agttgctaag aggcatatga accactttta cacctttttc
     3301 ttttaatata tctatttctt catcatttac ttttacacac tgagatgcta atacagtttc
     3361 atccaatata tctaaatcat tatagtagtc aactgctttt ttgccaaaat gtttttcagc
     3421 atagtttggc tcatatatac tctcacataa atgaaattgc attggtgcat ttaatttttt
     3481 tgcatcttct ttagctttct taataaatct atcagaacat gtgaagcttg tatgtgtaca
     3541 cattattcca tttattaatt tactattttc tctactccac tttattaaat tagaattttc
     3601 atctaaacat ctaagtccat tttcaaagct tattctttca caactttcta aggataaaat
     3661 cgctttcatg cctatttctt ctagtatttt tccttgctca attaaagttc cttcttctgt
     3721 atttggagct tctagggtat cacaaaaagc taccacacca gattctataa gttctattgc
     3781 tgatgcttta gttgttgcct tcacttcttt caatccaatt ctattttcta taaatggcca
     3841 ccaatagtca tttaaaaaac cttcaaagtc agtaaaatgt acatttgccg gtattcccct
     3901 agaaagaact ccatattgat gcatatgacc attgacgaat cctggcatta ttatagcatc
     3961 tgatttatct attatttctt caatattttt atattttatt tcaacttctt catttggcaa
     4021 tatatctttt ataatgtcat tttctactac agcagcagaa tttgtatata tttcatcagc
     4081 agaagatatt aagtatttac ttttaatagc aatcatttat ttacctccct gatttaattt
     4141 tttcatggcc atataaactt tctcagaagt tataggaagt tctcttatac gaactcctgt
     4201 ggcattgtat attgcatttg ctattgctgg tgcaggtgta tgaataacta tttctcctaa
     4261 agatttagct ccatatggac ctgttggctc ataattatct acaaaatcaa caattatgtt
     4321 tcctatatct ttttttgttg gaactttgta ttgtaataga ttagaagttt gaagctttcc
     4381 atcagaacca tgtctagggt cttcatacat tgcaagccct ataccttgag ttacaccacc
     4441 ttcaacttgt attctagcaa gtgcaggatt cataactgta ccacaatctg tagaacaagc
     4501 ataatttatt actttaaact cccctgttgt tttatctaac tctatttcag caaatcctgc
     4561 taagaatggt ttagcactct cttgtatacc ataagattca gatgaatata aaacttcttg
     4621 atttccacca ccaacagatt cttttcctaa ttcacttaac aaaacaaact tctctacatc
     4681 ttcactataa caaactctgt cttcatatag tattaaatca tctattggta aatcgagttt
     4741 cttctgtgct acttttaata tcttttgttt taaactttca gcagatttta ttgtagcatt
     4801 tccagttaca taagtagtac aagaagcgta tgcaccagta tcatatggac acatatctgt
     4861 atctcctgta taaacggaaa ttctatcaat agatgtattc aatgcttttg cagctatttg
     4921 tgcaagtatt gtgtcagaac cagttcctaa gtctgaagac ccagaaaaca acttataagt
     4981 tccatcttca tccatcctca tatttactat tgccatgtct atgttggcta taccagaacc
     5041 atgagttccc acagacattc caacagccct caccttattg tttccaatat ccctaacagg
     5101 gtacttttca tcccatccaa taagtttttt acctctaatt atacattcat caagtgcaca
     5161 acttcttatt ggaaaattca ttattccgcc ttcagtttgt ttttttataa tattctttaa
     5221 ctttatttct gttgggtcca tattaagttt atgcgctaat tcatcaattg cacaatcaag
     5281 tgcaaatgta ccttgtgttg ctccatatcc tcttaatgct cctcctggaa ctaaattagt
     5341 atatacagtt ctcccatcaa attttattgc aggtacatta ttatatgttg gaagtacatt
     5401 atgacctgat tcagagcaaa ctgaaggtcc attatcacca taagcgcctg tattattcaa
     5461 tgctttcata tctatagctt ttatgtttcc ttctttatct gccccaattt ttatatcaaa
     5521 aaacatctga tgtctagtat tagtcattgc aaaagtttct tctctagtat aaattagttt
     5581 tgctggtctt tttgtcatcc aagttacaaa tgatgcatat atctctgtta cagctatgtt
     5641 ttttccacca aaacctccac ctattcttgg ttttattact ctaacttttg aaagaggtat
     5701 tcccagtgct ttagaaacct gtcttctcat atgataagct gattggtttg cagaagttac
     5761 tactaatctg ccattcgtat ctatatacgt ataagctcta tgagtttcca tcatacaatg
     5821 ggcttgtgat tgagtcatat aagaatgttc tactattaca tcactttctt taaactcttt
     5881 ttctacatcc cctttaccca ttttttctct cgatacaata ttttttgtag catccaatcc
     5941 aaaatcaaat ggacaaaaca aatcatcttc ttcatggatt aaaattttat ttccttccga
     6001 ttctcttgca tccaatatag gagtcattac ttcatattca acttttataa gtttcatagc
     6061 tttaatagca gccctttcat cttcagcggc tattatagca acttcatctc ctacatatct
     6121 aactacgtct tcaagtatta attggtcatg aggtgatgct tctggatatg actctccaac
     6181 cattgaatat ctataatttg gaacatcttt gtaagtgaat atgtttacta caccttttac
     6241 ctttaatgca ttttttgtat ctatgttttt tatcttcgca aaagcatgcg gacttctaag
     6301 aagcttaatt gttaaaacat ctttatgaaa aattaaatct tctgtatata ttggttttcc
     6361 agtaacaatg gcttcactat ctatttttct tacctcttta tttaccatca cactcacctc
     6421 caatactatt atacattctc actactaagg cttttgccat atcttttctg tattctttac
     6481 ttcctcttat attacttcca aatgaaagtt cttcacttga tacttcacca gctttttcta
     6541 tatcattttc taaacttaat atttcacttg ctttataggc aatttttgct cttcttggtc
     6601 tagcgcctat cgcaatttta taaatacttc cttcttttaa cattgctcca tttaatactg
     6661 caaaatcacc tgtacatttt cttatagaat caaatacacc tatagcatct tttttaggta
     6721 atataacttc tattaatata tctttttcaa gctcactttc taaaaaatca gctaagttca
     6781 taaccccttt tttatataac ttaacctttg catctaccac tagtaatgaa ggtattaaat
     6841 ctgaaaatcc atacttagca aatacgctag ctcctactct ggctccactt ctaaattgaa
     6901 ctcctactat attagaaaca gcgtttgaaa ttaccccatt acagtaattt ttaataacct
     6961 tatttaattc taaagttctt aaacttgtgt ctgctccaat taatatgcta tcatcagttt
     7021 catttatata atctaaagct aagtctttta aatctattgc tgagccaata tttttactac
     7081 ctagttttaa aaatccacat cctccaagta ttatgttgtt ttttcttgat attagcagtt
     7141 cataagcttc atctaaactt tttggaacta catattcctt tatagttatc atactctctc
     7201 cccatatcaa cttaattttc acttaaaagt ctaagtgttt aaatatttat gtattgctct
     7261 taattgacct ttatatcctg tacatctaca aaggttacca tttacataat ctataacttc
     7321 ttcttctgtt ggattttgaa gctctctttt cattgccaaa accatcatta taaatccagg
     7381 agcacaaaat ccacactggt cagccccttc gtctgcaaga aaatctccaa acttctttgc
     7441 ttcatccaag accccctcaa gtgttgtaat atgctgacca tctactctag cagtaagata
     7501 attacatgaa agcacggcct tgtcatttac catcactgta cataacccac atgagcctgt
     7561 atcacaccct tttttaacac ttacatatcc attttctctt aatgtatctg ctaaaaaatc
     7621 atctggtctt atacttaatt ctttttcttt attatttatg gtacatttga taatcaaata
     7681 aatcacctca aaactcatat ttttaagcta aagttttact atacttttct atgaataaat
     7741 tacatttatt aataaaggct attccattta tgaccttcca ttctattttt tatagttcct
     7801 cttacacaaa gtctctcagc tgtatcttga gcatctttta atgctttctc ttcattagct
     7861 gtcaatattt ttgaatcttc cattattata tttccatcta ctataacact ctctatattt
     7921 ttcatactag aagaatatac taatgtagat accggattat gcataggtat cgcttttggt
     7981 gaaagcattg gattaaatat tagtaagtct gcttttttac ctatctccaa tgaacctatc
     8041 tcatcttcca ttccaatagc tcttgctcca tctatagttg ccaactcaag aactttttct
     8101 gccgacattg ctaatgggtc acacttatta actttatgtt gaagtgctgt taatttcata
     8161 agttcaagca tatcttgaga gttattacta gcggctccat ctactcctaa actaactgtt
     8221 attccttttt taagcatttc tggcactggt gcaacacctg aagataagta catattactt
     8281 gctgtattat gagacacttt catatcgtat tttttagtaa gttccatatc tttttcagtt
     8341 agatacacac aatgtaccat aagcacattt ggtccaagta ttcctaattt ttctaaaaca
     8401 tctatgtcat attgcccatg caattctttt gctgcttctc tatcaaaagg agtctctgat
     8461 atatgtactg taaataaagc atcatcatat tcttttacca ctctccatag catttcaagc
     8521 atttcttgag aatttgacca tattgctgca ggagctactc ctattttgat tcttccattt
     8581 tctgtattat ggtgtttttc aaacaatctt cttacatctt tttcaaccgt ttctacatct
     8641 tgcattattc caggatgaac tccaaattga gctcctgtat tcatacatcc tcttccaagt
     8701 attcctctta ttcctaattc tttataagca tctattattc catcacaaag acctggctta
     8761 ctatgaggat acatgtagtc aaccatggtc gttataccac ttctaagtcc ttcaatacat
     8821 ccaagcattg cagcatcata agtatctttt ggctctaaat agttagctgc tggaaatgtc
     8881 atagtttcta accagtcttt aagtaccata tcatctccca atccttttaa taaagtttgg
     8941 aataaatgat tatgtgtatt tataaaacct gggaataaaa ctttaccttt agcatctata
     9001 atttttttaa catcagtata tttactttct atctctttag aatttccaat atcagcaatc
     9061 ttgttatctt ttactactaa tgccccatca aaaataactt ctctctcttt attcacagtt
     9121 actattatgg cattcttaat aagtatatcc atcattctta tttccccccg cattatttag
     9181 taatttttct tttaatatat tgacctgttg gatgttcttt aacattacaa ctttgtacta
     9241 catgatttcc tctcaaaaat acatcttcaa ctttacattt aaccttaaat ccttcatatg
     9301 gagtataatc tacattttgt ttttgagttt tataactaat tgtttcttct ttattgatat
     9361 ttaaaatgac tatatctgca tcacttccta cagctatctc acctttttta ggatacattc
     9421 caaatatttt tgcagcattc gtagaagtaa cttccacaaa cttatttaca cttattctat
     9481 tttctaagac cccatatgta tataaaagtg ctaacctatg ttcaacacca ggtgctccat
     9541 ttggtatctt actaaaatca tcaattccca agtctttttg ccccttaaaa ttaaatgaac
     9601 agtggtctgt gccaactgtt tgaatatctc cactagacaa tccttcccaa aggtaattta
     9661 tgtcttcttt ttttcttaaa ggaggagaca ttacatactt agcaccctca aatttattct
     9721 tattatataa attgtcttct aataatagat actgtgggca tgtttctact atcatatcta
     9781 tatttttttc tctagccatt tttacagttt caagtgactc cctacaactt aagtggacta
     9841 cataggattt tgaattagat aatttagtta tatcagcaag tcttgaaaca gactctttct
     9901 caactatgtt aggtcttgta agtggatgat attttggctc taaattacca ttttgtatat
     9961 tttcttctat caatacatct agtaaatccc cattttcaca atgaaatcca actatacaac
    10021 ctaaatccct tgctttcttt aaaaccctgt atattgttcc atcatctact ttcatgccat
    10081 catatgccat atacatctta aaagatacta tcccatccga aataagtttt tccatatgat
    10141 taaatgtatc tttatttaaa catgtaattg tcatatgaaa accataatca caataacaat
    10201 ttccacttgc ttttttatga tatgcttcta caccttgcag taggttttcc ccctcattgg
    10261 cttctgcaaa atctagtatt gtagtagttc ctccagctat agctgctctt gttcctgttt
    10321 caaaattatc tgcagttgta atagaacctg cattcatatc aaaatgagta tgtgtgtcta
    10381 ttccaccagg aattacaatt ttacctgtag catcaataac cttatcacca tttatttcta
    10441 agttatttcc tatttcaact atcttttcat cttctatcct taaatcactt atataagtct
    10501 tatcagaggt aattatagtt ccacctctta gtattgttcc catatatacc tcctaacttt
    10561 taagctttaa agtattttct aagtatcgta aaactttatt taaaattgta ttttctaaag
    10621 gtgctatatt cagcacctct agactatatt taaataattt attgtgaact aaatattatt
    10681 ttccgtatac agattttaat attccatagt aaccttctgt tcctataaat aactgttctt
    10741 gctctatata ctcatctatt gtgtgagcaa gattttcctt agaaggacca aatcctatag
    10801 tttttattcc agcttcacct gcatagtgac ttccattcgt acagaatgag tattgagtta
    10861 tttctgaatc tattccagct tcttttaatc ccttatatgc tgcttgaaca aattcatctt
    10921 cttcgtcata taaccaacct gggaagaatc tttcactttc tatagtattt cctgtatagc
    10981 aatcagcttt ttcaaccgca taagaaactt tcgcatttaa ttctgggtct tctttcatca
    11041 tttcatctaa taaagcttgt attggagcta atacgccttc tcttgtttct ccaactaata
    11101 atcttctatc aaaagtagct ttgcaataat ctggcactac tgatgcccct ggatatggag
    11161 aagattttat atctgttaaa actaatattc catcacctaa aactggatga gttggaggta
    11221 ctaattgttg tatcttttgg attacattag ccattttata aactgcattt acaccttttt
    11281 ctggattagc agagtgagct ggtttaccaa aagtttctac tactatttct cctctacctc
    11341 tctgaccaat ctttaagttt aactctgaag actcacctat tacaacatag tcaggttgaa
    11401 cagccttact tatctctcta gccgaaacac cttcaaatat ttcttcatga acaacacctg
    11461 ctacatatag ttctccagca aaatctcgat tagtgtcttt agcaaaatac gctacagcag
    11521 acatcattgc agtatattga cctttcatgt cagaagttcc tctaccatat atctttccat
    11581 caactatttt tccttcaaaa ggaggaacac tccatttact tgcatctgga actggaactg
    11641 tatctatatg tccatcaaat aatattttct tacctggctt gttacctttt atacatccta
    11701 ctatatttcc atatctatct cttgaccaac tatcaaaacc taatttttca aaagactctt
    11761 ctattgcttt tacaacacct tcttcatttc cagaagaact aggattttga attaattttt
    11821 ggcatatttc agttacttct tgttttcttt gttcgtttaa catttcatta tccccctaga
    11881 ttttttataa tgtatgtatt ttatatactt atatattttc taaattttag aacttatata
    11941 tcttctaaat atgtaaacca atatctaata attgcatttt agtaattcct tatatacttg
    12001 gatattctcc atcccaaact atacttctat atttgtcagg gtcagtatct ccttctgtgc
    12061 ttattaaaag tattcttgaa ttttcatcta attttaaatc ttttcttaaa tctgctaaat
    12121 cttctctttc catagctgct actaaaagac cagttgtaac tgctccagat tctccagaaa
    12181 ttaccttttc atcaccattt aaaggattac ctaatgttct cataccttta gcagaaaccc
    12241 agtctggtgc tgaaacaaat gctgcagcat gattttttaa tacttcaaat cctatagtat
    12301 ttggttctcc acaagcaaga ccagccatta tagttggcat atctcctcca acaaatcttg
    12361 gttttccatc tcctgcttct gctgatttat agtagcaatc agcttcatca gcttctacta
    12421 ctactgttat tgggcattca tcgtatattg aagcaacata tccttgaact gctccagcaa
    12481 gtgaaccaac ccctgcttgc acaaatacat gtgttggtct atctactcca tattctttta
    12541 attgctctat cgcttcttga gccatagttc catatccttg cattatccat gctggaattt
    12601 cttcatatcc atcccatgct gtatcttgaa ccattactcc atgatcatca gcttctgcat
    12661 aatcattagc taatcttact gcatcatcat agtttaagtc tgttatacta gcatctgctc
    12721 cttctccttt aattttattt aatctaaact cagaagaacc ttttggcatt aatacaacag
    12781 atttttgacc taatttatta gctgtccaag caactcctct accgtgattt ccatctgtag
    12841 ctgtaaagaa agttatttct cctaattttt cttttatttc tttcgaagtt aatttgtcat
    12901 accctaactc agatatatct gtatctaatc tttgagctaa gtatctaccc atagaataag
    12961 aaccacctaa tactttaaat gcatttaatc caaatctata agattcatct tttacatata
    13021 ctcctgcaac acctaatttt tttgctaaac cttctaggtt tgctaatgga gtttctttat
    13081 attgaggaaa gcttttgtga aaatttctaa ccttagttat ttcttcttca tttaaaaatt
    13141 tgatacaatt ttccttatca ccctttggca aattatttac tttccattta atttctttta
    13201 acatctaaaa tacctccaaa ttattgttaa atttttatac tttaatatat aagcaattaa
    13261 catgccaaca ctaaatatat actagatatt tttctatgac tctttagtat agaaattgca
    13321 atgcttttat tattctttct tttctatctt tagttcatac aatgtatgta gaatttaata
    13381 actaaaaaac cttttctatc ttctcacttt ttaattctca tttttaaaat ttaaaaataa
    13441 atctcaaaac actttgtttc tatatttttt aatataaaat ttataaaatc taaattaaaa
    13501 cataaaatag tgatgcaata atctattaca tcactatttg tatatattta ataatataat
    13561 aaatctttag tggtctctaa gaggtgattt ttcatctttt ttaacatcta attcttcttt
    13621 tacgacattc catttttcaa gaccaaactt agaaaccata actgcctctg cagcaccttt
    13681 tccaagtctt tttactgtgt ccatacctgt ctcatcctct tgggcacctt ttgcttttcc
    13741 atctttactc catattgtac cacctatata gcatccacct aaacctccag atactaacat
    13801 ttcatgcatt aggaagaaat ttattatagg aagttttgtc aattcttggc caccatatct
    13861 agttccacca gtagttatag ctgcaccaac cttattttga agttttcctg gatgaactaa
    13921 atacattgga cgaagtctat taaatactgc tgtaagttga gcagttatat tcatatcata
    13981 aactggagaa cctactataa ttccatctgc ctccaatatt ttttgctcta attcttggat
    14041 atcatctttt ataatacaca tagtttcttt tcttatacat gcatcacaat gtacacaagg
    14101 acttattttt ttccctctaa ctgaccaata ctcagtttct ataccaggta tagtttctgc
    14161 ttctttcaaa gcttctaaca ctgcatattc tgttgctcct ttacgtggac ttccacaaat
    14221 acctaatatc tttgccataa taatatctcc tcaccaaata aatctatttt tattatctat
    14281 ttacaaatac aataaattgt tgtttgtgct ttaatttcta ctattttaat tttattggca
    14341 atgaatgtat ttctacatca gttgcattat atactgcatt tgcaacagct ggcgcaactg
    14401 gtacaacagc acattctcca atactcttac caccaaaagg tccagctttt tcataactat
    14461 ttacaaatgc aacttgacat tttggcatat ctacagttct aaataattta tatgttttaa
    14521 aatcagtagc tttaggtcta ccttgttcat caaatgccaa ttcttcactc aatgcgtatc
    14581 ctagacccat atgaatccca ccttctaatt gaccttctaa atttaaagga tttataactc
    14641 taccaacatc atgtacagct acaaaatcaa ctacttttat atccttagtt tctttatcta
    14701 ctaaaacttc agcaaaatgt acaccataag aagtaagtcc agctggtgaa gaataatctt
    14761 caacaacagt taactctctt aatgatttac tttgactata tactgcgaca tcagaaagtg
    14821 atgcttttac attttcatca agtttactaa ttacatatcc attgtctaga tataaatctt
    14881 cttttgatgt ttcaagtaat ttttctgctt cttctactat aaggtttttc aatttctctg
    14941 ctgtcttttt agctcctcca ccttctacaa atacccctct acttgcatag tcaccaaggt
    15001 tgaatgtaca tgcatccgta tcagtttcaa atgtatcaac tttatcaggt gttattccta
    15061 aaacttcagc taccatcatt gtttgcatag ttacgacacc attacccata tcatgtgctc
    15121 ctgttagtag tgtcaatgta ccatcttcat ttaatttaag agtcaatgta acaacatctc
    15181 tatgtgcacc aaaaactcca ttaccatgtg cacctattgc catacctata cctcttaaat
    15241 attttccttc ttcttttgga agttttttct tttcatccca cttaaacatc ttcttacctt
    15301 gctctataca atccaatact ctaggatttc caagagaacc gcggaagatt atatcgacat
    15361 catctggctc tactgcattt ttattttgaa aatcgactaa atccatacct atttccttgg
    15421 ctattttcgc aaattgagcc tgttgagcca taaatatttg aggtgaccca tagcctctca
    15481 tagctcctgc tattggtgta tttgtatata caggcatccc agtaaacttt atatttggta
    15541 ttttataaac tttaaatact ttatgactaa gtgctcctat aacatttaaa gcacttgatg
    15601 catatgctcc tgtatttgta tatattttta tatcttgtgc tattacttca ccatctttat
    15661 ttacaccagt ttttaatttt acaaaagacc catgacgagt tcttgttgat atcatagatt
    15721 cctttcttgt aagaaccatt ttaactggtc ttcttgtcat ttttgataat gcggctgcta
    15781 ctggttcaat tgtaagttct aattttccac caaatgagcc tccaatagca ggtcttataa
    15841 ctcttacttt atttaatgac atcccaaaaa tttgagatag tattattcta aatccaaatg
    15901 tattttggca aggtgtatgt acagttaatt tatcattata atcatagtgt gcaattacac
    15961 tatgagtctc tattgcacta tgatgtattg ctggagtaga gtattcatct tcaaatatat
    16021 agtcacattc cttgaagcct ttttctacat ctcccccatt tatgttgttt tgagaaacta
    16081 tatttgaatc tccatgtatg cagatagcat cttcttttat tgcatcttgt atagtaaata
    16141 cagctggcaa ttcttcatat tcaactttta taagttttac agctttctct gctatttctg
    16201 gagtctctgc agctactgct gcaacacggt caccaacaaa tctaactgta tctgaaaata
    16261 taacttcatt ttcaggtata ttgtgctcat aaaatcttaa tgaaccattg tattttcttt
    16321 gtgatgtatt gagataagta gctatagctc taacacctac tactttctca gcttcacttg
    16381 tatctatact ttttattttt gcatgtgcta caggacttaa aagcatctta gcgtaaagta
    16441 cattttgtgg tttcatatca gccacgtatt tcatttgacc agtaactttt aaagctgcat
    16501 cttttagagg gtatgatttt cctattacct tttccataat tatctacccc ctaatctagc
    16561 tgcagataat tttacagctt ctataatctt tacatatcct gtgcatctac agagattttc
    16621 tttaaaagct tctttaattt catcttcatt aggagatgca tttctattta aaagagcttg
    16681 tgttgttata atcataccag gagtacaata tccacattgt acagctccaa cttctataaa
    16741 agcttcttgt attggatgaa gattttctcc attagcaaaa ctttctatag tgtatataac
    16801 tttaccttcg cagttttttg caagaagttt acaagatttc atgtcaactc catctatcaa
    16861 aactttacat gctccacaac tgccactatt acaaccttgt ttcgctccag ttagatttaa
    16921 tttttcacga agtacatgca aaagagtttc attcttttca acttctattt catacatgta
    16981 atcattaata tttaatttta ttttttccat tttctcctcc tacagataaa ctaatgttgt
    17041 aatagaatat tattacaatc ttattttaat attagtataa gctattccac ttatgtcctt
    17101 ccattctatt agttacatta gctctcttac ataattcttc tgctgcaagt tgtgcattaa
    17161 gcaacacttg ttcttcactt tcaattgtag ttacttttcc attttcaagt attacatttc
    17221 catcaactaa aacactctct acattgtgca tactagctga gtataccaaa gttgatacag
    17281 gattgtgaag aggtattgat tttggagaaa gcattgagtt aaatattact aggtcagctt
    17341 ttttcccaat ctccaaagaa cctatttcat cttccattct aagagttctt gctccctcaa
    17401 ttgtagccat ttcaagaact ttctctgcag aaattgccag agggtcacaa ttatttactt
    17461 tgtgttgtaa agctgttaat ttcataagct ctaccatatc ttgtgcgtta ttacttgctg
    17521 caccgtcaag acctaaactc acatttatac ctttttttaa catttctggc acaggtgcaa
    17581 caccagaagc aagatacata ttacttgcta cattatgaga aacttttatg tcatactttt
    17641 taagcatttc catgtcttca tcagttatac atacacaatg tactgctaca acttctggac
    17701 ctactatacc ccatttttca agtaatttta tatctatttc tccatgtata tcctttgttg
    17761 ctgttcttgc aaattctgtt tcagatatat gaactgtaaa atatgaatca tattctttaa
    17821 ctatattcca aagcatctta cccatttctt cagatatagc ccacattgaa gaaggagcta
    17881 cacagatttt tattcttcca ttttctgaat tatgatattt ttcgaatacc cttctcaaat
    17941 ctttttcaac cgtttccaca tcttctataa gctctttgtg aatacctaaa tctatacaac
    18001 ctctacctac aacccctctt atacctaaat ctttatatgc ctttactatt ccatctgtaa
    18061 gtcctggtct attgtgagta tgcatataat caaccattgt agttatacca ctttttaaac
    18121 cttccataca tccaagcatt gcagcatcat aagtatcttg ttctgttaag aattttgctg
    18181 atggaaacat cattgtattt aaccactcat gaagtgccat atcatcacct aaacctttta
    18241 ataacacttg gaacaggtga ttatgagtat ttataaagcc agggaatatt acttttccat
    18301 tagcatctat aactttttta gcatcaagat acttaggctc aatatcaact gtatttccta
    18361 tatccaagat tctgtcacct tgaatagcta ttgctccatc aaatataaca tctctgtctt
    18421 tattaactgt aacaattatt gcatttttta ttagtatatc aatcatctta aatccccctt
    18481 tgaatacttt cattacaaca ttttaccata tggttttctc atattatatc taatacattt
    18541 atccaatatt ttcctcaaga tttgaagtaa cgttttcagc acttttacgt gactttaatt
    18601 taaataatgt ttgaagtaat atagctatta ttgctccaga aactatactt gaccctccaa
    18661 ggacagttcc aattgtttct gggaattggt ctaaaacttg tggagcagtt gtaagaccca
    18721 tacttgttgc tatagataca cctgctataa gattgttttc ttcagtgaat ccatccatac
    18781 tcataataga gaaaccagac attgctattg atgagaaaac tactaaagtt gctccaccaa
    18841 caacacaaga tggtatagtt gtcattattg cacccaatgc tggagatatc cctgctagta
    18901 ataatacaag tgcccctagt gctattacaa atctacttgt aactttgttc atagatacaa
    18961 ttactgtatt ttggctaaat actccagttg gaagagctgc aaatagtgat gaaattatag
    19021 aagttataga atttcctaaa acaactgatg ctaattcttc gtcagttacc tctctatcca
    19081 ttgctcctac tgtagctatt gtacaggcac ccatcatatc agctatacct attatgtaaa
    19141 taatagtaaa cattactatt aactctggtc taaattctaa tccaaaagct gctggtgttg
    19201 gtaaagatac tatagctgct ccttgaacgg ctgagaaatc aaccaatcct aaaactaaag
    19261 atatagcata acctactaat attcctatta atattgaaga attttttact aaacctttac
    19321 cgtatttatt tatcattatt attacaaaag caactataac tcctactgtg aagtttattg
    19381 ggcttccaaa tgtttgtgtg ccttctcctc cagctaaatt ctttatagct gttgcgaata
    19441 gtccaagtcc catacatgct attatagttc cagaaactat tggagtgaat atatgtctaa
    19501 ttttcttaac tccaaatcct acaaagaata cgattacccc agcagcaatc tgtgctccaa
    19561 atagagctgc taaaccatct ttagctgcta ctgaaagaca agctcctaaa aatacataac
    19621 tcattcccat cattacaggt aatcttgaac ctaacttaaa tcctttaaat aaaggtattg
    19681 gatataactg taaaaacgtt gctaaacctg ctgctaacat agaaccctga atcaacattg
    19741 ttgcttgccc ctggttaagt cctaccacgt ttgctattaa tatcgcaggt accatatttc
    19801 ctactatcat tgctaacaga tgttgtaagg ccaaaggtat tgattccttt agtggtggta
    19861 tcccatcgac atcatatttt gaagtattac gtgttttcat aaaataaatc ctcccataca
    19921 taaatatctt gactaaatat ttttataatt attttctata aatattttta tgaccatgaa
    19981 tttattgctc aaccgataaa cacagctaaa ctgagctatt tttataaaaa attaacaaat
    20041 ttctaaatat tccctccccg attttcaact taaatgaaac attaattgcc tggtatatat
    20101 ttatttgtta aattttttat taaaatgaat agctatcaaa aataatctaa caatctcata
    20161 ctaaataatt taaacaattc ttttaaatta ttatatctta attagtctct tccataacag
    20221 ctctttcttt agcctcatcc tctagacttt tatttggtat tattagattt aaaacgaatg
    20281 caaccaatgt tgcaattact attggagatt ctccaaatac taactttaca aatgaaggaa
    20341 atgcctcttg ggctgctggt actgatgtta ttcccatgcc taaggcaatt gccaaaccaa
    20401 caatggtaat atttcttgat gacaactctt ctgtaataat taactttata cctgtcattg
    20461 ttatcatact aaatatagat attgtagctc ctcctaaaac agaatatggt atagtagtca
    20521 ttaatgcacc aaatttaggc acaaaccccg acaataacat aaatatacca gctattccaa
    20581 gcacaaatct acttattact ttgttcatag ctaccatccc cacgttttgg ctatatgatg
    20641 atggtggtaa acctccaaac aaagctccta tcatagtaca aattccactt cctattactc
    20701 cacttgataa ttccttatct gtgatattcc tatccatgcc tcccattgta gtagctgata
    20761 aatctccaat tgcttgaaca gcattgatta tacttacaat tactactgag attattacag
    20821 gcatatcaaa ttccatccca aattcaagcg gtttaggtat cgcaaaccaa gttgcctctc
    20881 taatagggtc aaaatttatt atacccagaa ctaaagatac acaatatcca actataattc
    20941 ccaaaaatat tgctgccaat tttgcatatc cttttgcaaa ttgactaaga cctataacta
    21001 ccactaatgt tatagccgct actgcccagt tttgcataga ggcataagta ggactattta
    21061 caccacctgc catataattt attgctatag gatacaaaga taatccaatt gtaaaaacca
    21121 ctgttcctgc aactatattc ggaaaaaatt ttcttatctt ttttatgaat atccctatta
    21181 atactgtagc cactccccct attaattgtg cacccaaaat gcttgctaat ccataatttc
    21241 ctccgactgc tacaagtgtt ggtacataag taaaaccaac tccaaaaatt ataggcaatc
    21301 ttgaaccaat atttcctatt ggatatagtt gtataagtgt tgatatacca gctattatta
    21361 atgcatattg cactaaaagt gttatttgtg tagggctaac acccacagct cctcctacga
    21421 ctataggtac tgtaacagtt ccaactatca ttgctagtat atgttgcatt gatagtggca
    21481 aacactttaa aattggtggc cttccatcaa tatcaaacat agaaacattc ttttgatatt
    21541 tactcaaggt tatcactccc aactattttt taatgacagt tattattatc tcacacaaca
    21601 tcctttcttt ttttattttg tcatctcaat ttaatatgaa gcaattataa tgccaatttt
    21661 ttaacataat tgttttacat atataatttt ta
//
LOCUS       DAMJVX010000051        21545 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMJVX010000051 DAMJVX010000000
VERSION     DAMJVX010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21545
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.053"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            300..1571
                     /gene="serS"
                     /locus_tag="PC407_001737"
     CDS             300..1571
                     /gene="serS"
                     /locus_tag="PC407_001737"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2497187.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKHKLNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            1850..3355
                     /locus_tag="PC407_001738"
     CDS             1850..3355
                     /locus_tag="PC407_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2497188.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            3469..4167
                     /locus_tag="PC407_001739"
     CDS             3469..4167
                     /locus_tag="PC407_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2497189.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            4452..5540
                     /locus_tag="PC407_001740"
     CDS             4452..5540
                     /locus_tag="PC407_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497190.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            5779..6969
                     /locus_tag="PC407_001741"
     CDS             5779..6969
                     /locus_tag="PC407_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2497191.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIEYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYTIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            7060..8562
                     /locus_tag="PC407_001742"
     CDS             7060..8562
                     /locus_tag="PC407_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2497192.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            8634..9890
                     /locus_tag="PC407_001743"
     CDS             8634..9890
                     /locus_tag="PC407_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2497193.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            10062..12836
                     /locus_tag="PC407_001744"
     CDS             10062..12836
                     /locus_tag="PC407_001744"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2497194.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAQALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRSMNSNSKN"
     gene            complement(12901..13086)
                     /locus_tag="PC407_001745"
     CDS             complement(12901..13086)
                     /locus_tag="PC407_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2497195.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEVYKILQKLDIDFERVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            13458..14636
                     /locus_tag="PC407_001746"
     CDS             13458..14636
                     /locus_tag="PC407_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2497196.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMFTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIVSIMIKIWLSRFNKFVGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKDMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     regulatory      14762..14847
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            14960..17914
                     /locus_tag="PC407_001747"
     CDS             14960..17914
                     /locus_tag="PC407_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2497197.1"
                     /translation="MKKGNKKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEDNSTDNKKPEQTSEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQTPEGEKSIDNKKPEQIPEEDKSTDNKKSEQALEDEKPL
                     DNKNTEKTPEEDNLLEDENLLKVLEEELNEDYGFVVKINNNTIETESMKKISFNLTYT
                     PTSKGIQAGDSITFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGK
                     IGLEAYVKKVDEDTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEG
                     RKSTTVFMPVKNKDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEV
                     SDFNIEQSKNSISVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQED
                     DATVSIFDRGDNYLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGM
                     RLTTSGEDGHDFRVVEVPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTI
                     NLSKEEVESKFFIAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKK
                     EVDKKVLNSSDNQIVKYKINMSTYGVYDAGQVNLLDEVNPSLKISNIKYSDNLELKKE
                     AGDGKNTIRLVNKYEFKQIKEGEPVQSWVTFDADFTNVKVGETIQNVAQINGSSPPGV
                     ETTKQGYAFEAKKVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGVIQSSVKKPGI
                     YYLVEVMAPSGYEKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPVPPNTDEP
                     IVNPPVPPSTDKPRKPSNPSDTEDTIVINPPVPPSEDIINPPIPEILNPPVPPSEEVI
                     ETPVKQIVPIPEVVKPSVSEEKNNKAKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIV
                     GLVVLRRNKNTN"
     regulatory      18100..18185
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            18285..18947
                     /gene="zmp1"
                     /locus_tag="PC407_001748"
     CDS             18285..18947
                     /gene="zmp1"
                     /locus_tag="PC407_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HDF2497198.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHAYSTTIQQNKDTLSQIVVF
                     PTGNYNKNEANAMVNRLANIDGKYLNVLKQDNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAEAFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     gene            complement(19065..20027)
                     /locus_tag="PC407_001749"
     CDS             complement(19065..20027)
                     /locus_tag="PC407_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2497199.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHIFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVLSPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(20197..21387)
                     /locus_tag="PC407_001750"
     CDS             complement(20197..21387)
                     /locus_tag="PC407_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2497200.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
BASE COUNT         8517 a         2495 c         3683 g         6850 t
ORIGIN      
        1 tatataaaag ttaataacta tacaagattg ataaagaaaa agtcaaaata gataattgat
       61 tagcaaaata gatataatta taatgaagtt aaatctagca atattctcct aaaattaaat
      121 ttcatttatt taattaaata taatttgaca atggacaaaa aagtgagtaa aatataaata
      181 aaataaaaat aacaggttcg gataaagaaa aaggtttctt aacctcaagc ataaagtata
      241 ttatctaagt tatttgagat taagagataa atccaatctt aggtactgag gaggaaaaaa
      301 tgcttgatat aaaaagaata agagaaaatt tagattgtat aaaaaaagca atggaaagaa
      361 ggggcgaaaa agactttaac cttgatgaag tagttaagtt agatgatgaa agaagaaaga
      421 ttcttcaaga agttgaagtt atgaaacata aattaaatac agcatctaaa aatataccaa
      481 atcttataaa agaaggtaaa gatgtagaaa atgaaaagat taaactaaaa gagttatctg
      541 ataaaattaa ggtaatagac caaaatctta aagaagtaga agataagatg gaatacctat
      601 taatgagaat accaaatgta ccacatccag aagtaccaca aggtgaaact gatgaagata
      661 atgttgaggt aagaacttgg ggaaagacta ctacctttga ttttgaatct aaggctcatt
      721 gggaaattgg aacagaatta ggtatacttg attttgaaac agcgagtaaa ataactggtt
      781 caagattcac tctttacaaa ggattgggtg ctagattaga gagagcatta ttaaattttt
      841 acttagacac gaatacaaaa gtaaatggat atacagaagt cattccacca tttatggcaa
      901 atagaaatag ttttttaggt acaggacagt tacctaagtt tgaagaagat atgtttaaaa
      961 tagaaggtct ggactatttt atgattccaa catcagaagt tcctttaact aatatacatg
     1021 ctaatgaaat acttaaattt gaacaattac caataaatta tacagcttat actccatgtt
     1081 ttaggtcaga agctggttct gctggaagag atacaagagg tttagtaaga cagcatcagt
     1141 ttaacaaagt agaaatggtt aaaatagttg caccagaaga atcttacaat gagttagaaa
     1201 agcttactaa caatgcggaa actatgctac aattattgaa tctgccttat agagtagtaa
     1261 agatatgtac aggtgattta ggatttacgg cttcttttaa atatgatgta gaagtttgga
     1321 tgccaagtta caataggtat gttgagattt catcttgttc aaactgcgaa gatttccaag
     1381 ctagaagagc aggaataaga tttaaaagag ataaagattc taaagctgaa tatgtacaca
     1441 cattaaatgg ctctgggtta gctattggaa gaagtgttgc agctatatta gaaaattatc
     1501 aacaagaaga tggttctgtg gttgttcctg aagttttaag accatatatg ggagtttctg
     1561 ttattaaata agagttaaat tatgtatttt taagcaatac aataatatag aagtaataaa
     1621 aaataattaa tataaaagtg atacaataat atagaagtaa taaaaaaata gttaatataa
     1681 aagtgataca ataataatag aagtaataaa aaaaaataat taatataaaa atgtgtacaa
     1741 actaatgaat attgatatag taagctatca tatgaaagag tatgtacaca ttttttttac
     1801 taatttttca caatatgaac ataaataatt tacaggaaag gaatctttta tgaaaattca
     1861 aaatactgag tggggatata ttgaatggaa acatacttat gatgaaaata atccaaaaca
     1921 agctatgaat atatacattg cagtaactat gcctggtaag aaacatttta atcatgttca
     1981 ttatggtcaa gagcagatga tatatatttt agagggagag ggtttgtata ttataaatgg
     2041 agtttggaag ccattttatc aaggtatgat tttttatata gaatctggta gcacacatga
     2101 aactataaat acaggcgata gagaaataaa agaacttatt gtttcaaata atgtagatga
     2161 tgtaggagag agtgaagtta tagatattaa tcctaataat tatttaaaga agaccttgat
     2221 aaattatagt gaaagtacat taaatttgta tgcagcagta gaatcaattc gtggtcaatt
     2281 tatagacccg tttaaaattc cattaattat atatgatgat tcatggaata tagttttaaa
     2341 aaacccttat tttccacttt tttgttttga aaaatgtaat cctatgaaat ttcctcaaaa
     2401 ttgtgattgt atgaatcaaa aaagtagcaa tcaatttgta tgtgaatatg ggataactat
     2461 atataatata ccaatactgt acaaaagcaa tagcataggt gttataagag gagggtatgt
     2521 actattatct gatttaaatt tagatacaga gcataataat ttatatgata ttccagaggg
     2581 agctgctaga agtataaaac gcctcttgaa acaaatatct aaaaatataa taaatttttg
     2641 ctcttttaat gatataagaa aggatttaca agaaaaagag aagactatag caagaacata
     2701 tcattatgga gagcaacttg aaatgaattt aaaagtagct caagatatgg ttactaattt
     2761 aagaataaat catcactttt tatttaatac attaaattca atggcaagca tagcactaga
     2821 tgatggtagt tatgatttgt atagtgcaat aatagattta tcaagaatgt ttagatatac
     2881 aatgaggtct gatttaagat ttgtagaatt agaatctgaa atattataca ttaaaaatta
     2941 tttgaattta cagaaattac gttatggaga tgcacttaaa gtaaaatatc ttattccaga
     3001 gaagttatat aacctttctg tgccttttaa ctttattcaa cctatcgtag agaatgcatt
     3061 tacacatgga tttagagata ttgacacaga aaagcgaatt gaaataattg caagattaga
     3121 ttctcagtat gctattatag agattcataa taatggaact atcttagatg gaaataatat
     3181 agacaaaata aaggcaggta ttcgtagtaa taatggtcat ggtttgtctt taatatatac
     3241 aaaattcaca tctgcttatg gaaataattt tgatatggat ataaagtcga gtgataatga
     3301 aggaacttac ataatgatta aaattcctat tgaaaattat aaggagtatg tatagaactt
     3361 gtgaaaaatt aataagtttt ataaatgtta agaaatatat tagatgtttt tatgttgttt
     3421 tatatatctt acttttttat attattaagc taaaagggag gtattctcat gagaagagca
     3481 ctaataatag atgatgaaga agctgttcta aaaataataa cacattttat agaaatgaaa
     3541 gatatgccaa taaaaattgt tggtaaagca acatctggtg atgaagcagt agataaaatc
     3601 attggtttag agccagacat agtttttata gatattcaaa tgccaatata caatgggtta
     3661 gaagtaatag aaaaaacatc tcatttatct aaaaaaataa actttgtagt aataactgca
     3721 tttaactatt ttgaatatgc tcaaaaagct ttgagattaa atgtaaaaga tatactcctt
     3781 aaaccattgg atatgaaaga atttactaag tctgtagaaa aaattattgg atatcaatat
     3841 accaataatg atttattaaa tgagatactg gaatatataa atctaaacta tagcaatgac
     3901 ttaaagttaa atgattgtgc tagattattt ttaaccaatc catctaatat atctaggatt
     3961 tttaaaagta atttaaatac aacttttata tcatatctaa atcatataag aatagaaaaa
     4021 tctattgaat tacttgaaaa tactacaaaa agtataaatg agattgcaga aatagttgga
     4081 tataatagcc taaataactt ttataaaaac tttaagatag aaaaaggaat gactccaaaa
     4141 gtttataaac tgaacactaa aaattgaaat tgataaataa aaagtcaata tagataattg
     4201 tttgagtgtg tctgaataag tatactttaa ttatgataaa aattaaagcg ctttaaacat
     4261 atttaataat attaaacctt ataaaagctg tacctattta tataaaaaaa tatatataaa
     4321 taaaaagaaa aagatataca aaataaaaaa agtgaattaa atactgtttc aaaaagacag
     4381 aattttcatt taattcactg agagtttata tttcttaaag gaaggtaact ttataatgga
     4441 ggtaatttaa tatgtcagac aaagtgaata tgaaaaatat tatctgtatt gcaggcgctt
     4501 atctagcaac agcaattggt tctgggtttg ctactggtca agaaattcta caattttttg
     4561 cctcacaagg gtatatgggt ttaattggag cattaatatc aatggtattg ttttcatttc
     4621 ttggtgctga agttataact agaggtagag aattaaaact taaagaacca acaagaattt
     4681 acacttttta ttgtgggaag attttaggaa aattttacga atggtttagt ccaatatttt
     4741 tatttgggat tcttatagta atgatttcag gtgcaggctc aactttaact gaatactatg
     4801 gtataaatcc ttacttagga agagtattga tggctgttgc tactttaatt acagtgtcta
     4861 tgggtttaga aaaattagta agcatacttg gacgtattgg accagtgata gcagtattta
     4921 caattgctgt tggattaata agcttgttta agcattttga tggtttatct atggttggag
     4981 aagctatgaa aaatattgaa gttacaaaag catcagaaag tccaatcgtt tctggtgtaa
     5041 tatataatac attaaatatt attgtaatga ttgttttttt aacaggaatg ggagcttcta
     5101 ttgaaagtaa aaaggatgct ttttggggag gtttattagg tggaatagtg tttatgactg
     5161 ctggtatagt tatgtatttg gctatgattt cagatatagg aaatttgtat atgaaagaaa
     5221 taccatcact ttatttagca gataatatat ctcctattgt aggtgtatgt ttttcagttg
     5281 cattaatatt aggtatctat acaacagctg ttccactttt atggtctgta accaatagaa
     5341 ttgttgaaga tgaacatcct aaatttaaat tggtaacaac agtaatagct attttggctt
     5401 gtataggtgg ttttttaccg tttggtaaat tagtaaacat tttatatcca tatacaggtt
     5461 atatggggct tttaatttta gtctgtatgg tgtataaagc aattacatgt agaaaaaaat
     5521 caacacaaag tcaggaatag agtcacctat taatagtttt tatttaaaga aattttttat
     5581 ataatccgaa tttaaaaagt caaaatagtt aaataaattg caaaacatga taatatattt
     5641 aattaaaata atatagtaaa atataaatat aaactaaaat taaatttata aaaaatatct
     5701 ttataaagat tattaactta tataatttgt agttaaatta taaaaaataa aaaaataaaa
     5761 ttttggagag gtgttagtat gcatgaactt attttaatct cagaaaagta taaagaagat
     5821 attttagatt tttttacaga tattcatatg caccccgaat taagttttaa agaatttagg
     5881 actacaaaag ctataaaaga tttattagta agtttagaca tagaaatatt agatttagga
     5941 atggaaacag gagttgttgg attattaaaa gggaagtatg atggtcccac agtagcactg
     6001 agaggagata ttgatgcatt gccaatatat gaagaggttg atattgagta taaatccaga
     6061 tatgatggta taatgcatgc atgtgggcat gatatacaca catcttgcct tgtaggatgt
     6121 gcatatgtat tgtctcatat tagagatagt cttcatggaa atgtaaagtt tatatttcaa
     6181 ccagccgaag aagtcaataa aggtgcgaag atgctagtgg aacgaggagt aatggaaaat
     6241 ccaaaagttg atgctatatt tggacttcat aatcatccag atataccttg tggaaaaata
     6301 ggtgttaagt taggtgggtt aatggcagca gtagacacta ttaagataga agtaaatgga
     6361 tttggaggtc acggaggtat accaaacaga acaatagacc ctatagtagc atcaagtgca
     6421 attataatgg gtattcaaac catagtaagc agaaacataa gtccattaga atcagctgtt
     6481 atatcaatag gtactataaa tggagggact gcaaataatg taatttcaga aaaggtagat
     6541 atgacaggta catgtagaag ctttagtaat gaagtacgaa agaaaatatc agaaaattta
     6601 gaaaatatag tttgtgagat agctcgtgga tatcaagcaa ctgcaaagct agattattta
     6661 tttgatttac cagctgttat aaatagtaaa gagatgtaca caatagcttg taagtctgtg
     6721 tgtgatttgt attcagaaga tgctatagtt gatccaatac cttctacagg aggagaagat
     6781 ttttctatat ttatggaaaa ggccccagga tttttttact ggttaggggt aggcaataaa
     6841 gaacaagatt gtatatatca gtggcataat cctaaattta aagctgataa aaattctata
     6901 ttggtaggta caaatgtact atgtcagtct gttataaatt atatggataa attaaaaaat
     6961 aaaatttaag atagagcatt ctggagataa agtttatgag ggggtattag tttgaggaaa
     7021 attaattcac aggaaaaaaa caaagatagt ggattattga tgagggcact aaatgttgta
     7081 gagagagcag gaaataaact tccagaccca gtaaccatat ttttattact atgtataatt
     7141 gttgtaatat tgtcagcagt tatatctaat ttaggagtag aagaaataca tccttctaca
     7201 aaagaagttg taaaggtagt aaatctacta gagaaagagc agattcaatc atatttaggt
     7261 agcatagtta caaacttcca gtcatttgct cctttaggat tagtactggt tacaatgctt
     7321 ggagcaggtg ttgctgagaa atctggattt atggaagtgt taatgaaaaa gggtataagt
     7381 aaagttccac aaaaacttgt aactgtagca atcgtatttg caggtatgtt atcacataca
     7441 gcagcagatg taggatttat aatattacca ccacttgcag cacttgtgtt tttaggaatt
     7501 ggaagacatc ctcttgtagg tatgtttgca gcttttgcag gtgttgcagg gggatttgca
     7561 gcaaatgtta tgcttagtac tacagatgta ttgttagctg gatttacaat accagcagct
     7621 caaatgatgg acccatctta ccaaggcaat ccagcaatga atctttattt tgcttttata
     7681 tcagcgatag ttttaacttt tgcaggagca tttgtaactg aaaaatttat agctcctcgt
     7741 tttacaaaat ataatgattc aacaaatgat aaggatatac aggaattgag tgaacttgaa
     7801 aataagggta taaaatacgc tttattatca ttgttagttg tagtagtagt gatagttgcc
     7861 ttatgtatag gtgacaatgc atttatgaaa gatccagaga ctggttctat actatctagt
     7921 aatgcacctt taatgaaagg tatagttcct ataatcacta ttatattttt aactccagga
     7981 ttagtgtatg gaaaagtaag taagaagata aaaagtgaca aagatttagt atctatgatg
     8041 ggtagtagta tgagtgatat gggtggatat atagtcctag catttatagc atcccagttt
     8101 attaatttat ttaatcttag taacttagga acaattttat caataacagg agcaaaatta
     8161 ctagcagagt cggggatacc tagctatgga ttgataatag gatttatatt gctatctgga
     8221 tttatcaatt tatttgtagg aagtgcttct gcaaaatggg cgattttagc acctatattt
     8281 gttcccatgt ttatgctact agactttaat ccagcattaa ctcaaatagc ttatcgtatt
     8341 ggagatgctt caacaaatcc aataagtcca ctttttccgt attttccagt tatattggcc
     8401 tttgccagaa gatatgataa agatataggt ataggtactg tcatatcgaa tatgattcca
     8461 tactcagtgg tatttacatt aatagaaata ataatattgt tactattcat gggtattggc
     8521 ataccattag gacctggtgg aggaatatct tacgttttat agaccattat aataaattta
     8581 tcaatgaaga ttaaactaat aaaatatatt ctttaattaa gaggaggatt attatgttaa
     8641 aaacaacaaa aatttcagac ccagagttat acaagattgt agctgatgag ttggttagac
     8701 aagaacacaa tattgagatg atagcttctg aaagtacagc accaacagaa gtacttgaat
     8761 taagtggatg tgtatttaca aataaaacag aagaaggcta tccaggggct cgttttcaag
     8821 caggttctga agaagcagat aaacttgaaa ccctagcaat aaaacgtgca aaagaagtat
     8881 ttggagcaga acatgtaaac gtacaaccgt attcaggttc tacagctaac tattgtgtat
     8941 attcatcaat attaaaacca aatgacactg ttttaagtat gcgtcttgac caaggtggac
     9001 acttgacaca tggtagcgca gttaattttt tacatgacat ttataaatat gaattttatg
     9061 gggtagaccc aaatactgga cgtatagatt atgatgctct tgaagcaaag gcaaaagaat
     9121 gtagaccaaa acttataatt gcaggggcaa gttcatatcc aagattaata gattatgaaa
     9181 gaatatcaaa ggttgcaaaa gaagtaggag cttactttat ggtagatatg gcacatgttg
     9241 caggattagt cgcagcgaaa gtaataccta gtcctgtacc ttatgcagat tttgtttctt
     9301 catctacgac aaagactttc tgtggaccaa gaagtggtat tgtactttgt aaagcagaac
     9361 atgcaaagaa actcgataaa ggtgtttttc caggaacatt agggtctata catcttaata
     9421 cagttgcagc aaaagccttt tcactattat atttaagtac agataaattt aaaaagataa
     9481 tggagcaagt tgtagtaaat gctcagactc tagcttctga actgatttca catggtttta
     9541 gtattgtaag tggaggtact gataatcata tagtaatggt tgacttaaga agtaagaatc
     9601 ttacaggaaa acaatttgaa aaagctcttg aatatgtagg aataacagtt aataaaaatg
     9661 ttatacctga cgacccacaa tcaccatttg taactagtgg tgttcgtata ggtcttacat
     9721 caatatctca aagaggtctg aaagaaaaag aagttataca gatagcagga ataatgaata
     9781 aggtagcaga aaatattgat aataaagaag tattagatga atgtaaagca gaggcacaag
     9841 aacttatatc taagttccca ttatatccag aagggtattt tgaagattaa atattttgat
     9901 ttgtatagct cacaatttat aaagcctgta acataaaatt taataatata taaccactat
     9961 aaggtgttga ttaatcaaca ccttattttt tttgaaaagt aatatacaag ctatcataag
    10021 catataattc cttatagata attaacaagg gggataaagt tatgaggtat tataataaac
    10081 ctaccaaaga agtattaaaa tacttaaaaa caaatccaga aattggactt gatgataatg
    10141 aagtagaaga aaggaagctt agatatggat taaatgaatt tactataaaa gaaggtagaa
    10201 cattttggga cgagttggga gaaagtttga cagaacctat gatattaatt ctcataggag
    10261 cagcagtaat aagttctttt gtgggagaat tacatgatgc attaggtatt ttaggggcta
    10321 tttttattgg aatttctata ggcataataa ctgaaggaaa atctaaaaaa gcagctcagg
    10381 ccctgtcaaa attaacagaa aatatagaag taaaagtact tagaaatgga aagataatta
    10441 aaatatcaaa aaatgattta gtaccaggag atattgtata tatagaaact ggagatatga
    10501 taccagcaga tggaagactt atacagtcta taaatttgaa acttagagaa gatatgctaa
    10561 caggagaatc tgatgatgta gctaagaatg ctgatgctgt attagatatg gaagtagttt
    10621 attctaaaac agaaataatc gaacaggatg caatacctgc aaagcaagtc aatatggtat
    10681 ttggagggac tcttgtagca tatggtaggg gcataatggt tgtcactcat actggtgata
    10741 agacagagat gggtaagata gcacagaatt tatcaaatga agaccagcaa actccacttc
    10801 aaattaaatt agggaagttg ggtgctaaaa tagcaggaat atcaggcgta atagctactt
    10861 tattgtgtat gtttatgatt atacagatgc agagaaaagg tatgttaatt ttagatacaa
    10921 gcagtatatt atctttctta caatcattag aacctgctaa aaatgcatat atggtttgta
    10981 ttgcattaat agttgcaact gttccagaag gtttgccaac tatgataaat attaccttag
    11041 ctataacaat gcaaaagatg gcaaagataa atgcattagt aacaaagaaa gaagcctgtg
    11101 aaactatagg ttctgtttct gtaatatgtt ccgacaaaac agggacttta acacaaaaca
    11161 aaatgatggt agaggtagca tatgttgatg gtaaatatat aagtggagga gagtatcaaa
    11221 gtaacagcta ttttgaacaa aactgtatag taaattcaac tgcagatata gaaaaagaag
    11281 ataattcatt taaatatata ggaagtgcaa cagagtgtgc attactttta tatcataatg
    11341 ataagaacta taatgaaatg agaaaacaaa cgtatttgat atcacaaata ccatttagtt
    11401 cagaagaaaa gaagatgtca acattaattc gtcaagagga ttcagatata ttactttcaa
    11461 aaggtgctcc agaagtatta ttaaaaaaat gttcttatgt acaacaagga aaaaatattg
    11521 ttccaataac tccaaaagta gaaaaaagta ttttagatga gattaagaag ttacaaatta
    11581 aatctatgag aacattagga tttgcttata aaaaaatgag taattctaaa acagaagttg
    11641 caatgacttc agaaggtgaa ttgaatttaa taggaaattc aagaagttat atgaaggaag
    11701 ataatttagt ttttagtgga tttgtaggaa tagtggaccc attgagagaa ggggtcaaag
    11761 attccattga taaagcattt aatgcaggag tagatgtaaa aatgcttaca ggagataata
    11821 taaatacagc aacggctatt ggaaatgaat taggattatt aaatgatggg aaaaaggcag
    11881 ttgaagcaac atatatagat gtattgacag ataaagaact gagagaagaa attaaaggaa
    11941 tatctattgt tgcaagaagt aaaccagata caaaaatgag aatagtttca gcgcttcaaa
    12001 aaagtggcga agttgtagct gtaacaggtg atggtataaa tgatgcccca gctcttagtc
    12061 aagcagatgt tggaattgca atgggtattt caggaacaga agtatctaaa aatgcagcag
    12121 atataatatt gactgatgat agctttagta ctatagtaga gggaataaaa tggggaagag
    12181 gtatatatga gaactttcaa agatttatac agtttcagct tactgtaaat atagtagcat
    12241 ttataattgc tataatatct caacttactg gtaaggatat gccatttact acaatacaat
    12301 tattatgggt aaatataata atggatggtc ctccagccct agcattagga ttagaacctg
    12361 ttagagatta tgtattgaaa agaaaaccaa taaatagaca ttctggaata atagctaggt
    12421 ctatgtttgt aaatattatt ataaatgcta ttcttataat tacaattgta tttactcaat
    12481 cagcttttaa tatattggga gctacatcag aagaacaagg tactgttata ttttctttat
    12541 ttgcgttcag tgcccttttt aatgcattaa attgtagaga atttggttta aatagtacaa
    12601 taccaaattt ctttaaaaat aaactggcat tgcaaataat agtagttaca gggataattc
    12661 aaataatatt tacacaagtg ttccagagtt tctttaattc tgtttctttg gactttgata
    12721 tgtggataaa aataatacta tttgcatcca ctatattatt gtcaaatgaa tttgtaaagc
    12781 taatacttag aacaatgaaa aatagtagaa gtatgaactc taatagtaaa aattaattct
    12841 tatgaaaccg caaagtacta tatattgtaa ctttgcggtt tttgtttgtt taatgttgtt
    12901 ttatattacc attttcatat ttaatttttt attaatctga acacaatctt ccattgtaat
    12961 tgcttcatct gtgtcaacac gttcaaagtc aatatctaac ttttgtaata ttttgtatac
    13021 ctctttttgc aatggagtat caaatttaat aggtgatttt gtgtaaatat cactgataaa
    13081 aaacatgatt tatttcattc ctttccgttt aaaatcttat agttatagtg tatcataagg
    13141 ttataaaaac ataaatatag tataaattta aaatatgtaa agacaaaaca aaaaaatcat
    13201 attctaagta atacttggga aaaatgatat tatcataata aataatcttt gtaaaaactt
    13261 atatagaatc tattaaacag acactaagcc ataaaaactt tacgaaatat aaaatttgta
    13321 tctaaataga tatatagaat aatattttac tgaagtagag tgatttttaa gaattttata
    13381 tataaattat gttatcataa ataaagatat agattataaa atttgttaat aaaataaagg
    13441 gcagggaggt aagtttcatg ttttccaaaa tcctagtcaa aacatttatc agagatagcg
    13501 aaaacgttca aaatacagat gttagaaata agtatggtta tgtagctggt gtagtaggta
    13561 tattgtccaa tttactttta tttgtgataa aggtattcat aggaatgttc acatctagta
    13621 tagctataat ggctgatgca tttaataatc tttctgatat ggcatcttca gcaataacta
    13681 tgataggttt taaattagca agtaagccag ctgacaaaga acatcctttt ggtcatggaa
    13741 gaatagagta tttatcagca ttaatagtag catttatggt gatgttggtt ggacttcaat
    13801 ttgtaaagtc atctatagaa aggatagtaa atccaattcc tgtaaaattt gaagtaatac
    13861 cacttatact tttaattgta tctataatga ttaaaatctg gttgagtcgt tttaataaat
    13921 ttgtgggaaa taaaattgat tcttcagcct taaaagcagt ttcacttgat gctttaggag
    13981 atgtatttac ttcaagttgt gtagttatat catttattgt agctagattt actaattttc
    14041 ctatagatgg atatgttggt atcgtagtat cattagtgat actttatgct ggtttctctc
    14101 ttgtaaaaga tactataaat cctcttttag gagaagcacc ggatgaagaa atggtaaatt
    14161 ctattataga gctattgtta tcttataaat atataatagg tacacatgat ttaattattc
    14221 ataactatgg agttggtaga tgtattgcat ctatacatgc agaaattcca tccaacatag
    14281 atataatgga aatacatgaa attatagata ctgcagagag agaaatatct gaaaaattag
    14341 atatatatct agtaattcac atggacccaa tatgtcttga agataaagaa gttatgtcag
    14401 ccaaaaaaga acttgaagaa atacttaaga aaaatagtct tgtaaaatct atgcatgatt
    14461 ttagaatagt tggaaaagga agcaagaaga atttaatatt tgatatagta gttaatcctt
    14521 ccgaattttc caaagacatg tcagaggatg acttaaaaga agatataaca aaattagtta
    14581 aggagataaa tcctgaatat aattgtgtaa ttgttgtaga taaggatttt atttaattat
    14641 taattaatct cataaaaggt aaaaaatgta aaaaagatag acaatagtat agaggcataa
    14701 tataatagaa gtatataatt gtatttttaa tataaataaa gcaattttaa ttgtttttat
    14761 gaaatattat agagatgttg aagtatattc tattattggg caccttatgg atatactgag
    14821 tcagtggtgc aaccggctat gaatataaat ttatttattt tcatagcttt ttttatataa
    14881 ttttttagta aaaattaatt tatatacgat attaatggaa gattataaac tatagataac
    14941 ctagaggggg aaaatttata tgaagaaagg aaataaaaag gcattattaa ttagtttaat
    15001 tatgatttta agtatggtgg tgtctacaat atatccaact gtatcttatg cttcagaatt
    15061 aggagagaat agtcagattc aaagtggttc aactaattca tcaactggag aggagaagga
    15121 aagcgacaat aaaaaaccag aacagactcc agagaaagat aaggcaacag ataataaaaa
    15181 accagaacag accccagagg aagataattc aacagataat aaaaaaccag aacagacttc
    15241 agaagaagat aagtcaacag ataataaaaa gccagagcaa actccagaag gagaaaagcc
    15301 aatagataat aaaaagccag agcaaactcc agaaggagaa aagtcaatag ataataaaaa
    15361 gccagagcaa ataccagaag aagataagtc aacagataat aaaaagtcag aacaagcttt
    15421 agaagatgaa aaaccactag acaataaaaa tacagaaaaa actccagagg aagataatct
    15481 attagaagat gaaaatttat tgaaagtttt agaagaagaa cttaatgaag actatggatt
    15541 tgttgtaaaa attaataaca atacaataga aacggaatca atgaaaaaaa tatcatttaa
    15601 tttaacttat actccaacat caaaaggaat acaagcagga gattcaataa cttttaaggt
    15661 gccagatgta ttcaataaag ttaatttaga ttatacaagt gaatgctttg ataaaacaga
    15721 aagtaatgga gagtatacac ttacatttag agaattacct aatggccaaa gtgtaatgca
    15781 aggtaaaata ggtttggaag catatgttaa aaaggttgat gaagatacta atgcaaaaat
    15841 acatattgaa accactggaa aaatagaaag tggaagtgga gatatagatg tagaaataaa
    15901 acctggagat aaaacggatg ttccagatgc taaaggaaca cttaaaaagc ttgttgaagg
    15961 aagaaaatca actactgtat ttatgccagt taaaaataaa gatataaatt acagtataca
    16021 agtaaatgaa aagcaggaag agttaaaaga tattattttg tatgatgagc ttcctgaagg
    16081 attgacattg ataaatggtt ctgtaagtgt agtaacaagt gatggaaaag aagttagtga
    16141 ttttaatatt gaacagagta aaaattctat ttcagttaat tttggaaata tagataaatc
    16201 gtatactgtt aagtataagg ctagaatcag tgacaaaaat gcaaaacatg gtaataaata
    16261 taagaatgta gctaggattg aatctgatgg taagaagata caagaagatg atgctactgt
    16321 aagtatattt gatagaggag ataattatct tcttactaaa ggacattcag gagcaacaaa
    16381 tattactcaa gttggtcaag taataaacta tcagatttca ataaatgatg ataaatcacc
    16441 aataagtaat gttgttataa cagataatat accagaaggt atgagattga ctacttcagg
    16501 agaggatggg catgatttta gagttgtaga agttccaatg aatgggagct ggactccttg
    16561 gagtaaagaa aaaattgcca ataatatttc atataaagta gaagaaaaaa gaaatgaaag
    16621 tgggcaagta gataaagtta taactggatt tacaattaat ctcagtaaag aagaagtaga
    16681 atcaaagttt tttatagctt atacattaaa ggtaattagt attgaagata gttatataaa
    16741 tagagctgtc cttgatgcaa ataatagtga aattgataaa aatgatgaga taaactttaa
    16801 gaaaaattct ggattaataa gcgcaaaaaa agaagttgat aaaaaggttt taaatagtag
    16861 tgataaccag attgttaaat acaaaatcaa tatgtcaact tatggggttt atgatgcagg
    16921 acaagtaaat cttttggatg aagtaaaccc atctctaaaa atatctaata taaaatattc
    16981 tgataatctt gaacttaaaa aagaagctgg agatggaaaa aatacaataa gattagtaaa
    17041 taaatacgaa tttaaacaga taaaagaagg ggaacctgtg caaagctggg ttacatttga
    17101 tgcagacttc acaaatgtaa aagttgggga gactatacaa aatgtggctc agattaatgg
    17161 ttcaagtcct ccgggagtag agactactaa gcaaggttat gcttttgaag ctaaaaaagt
    17221 ggatgcttta gataaaaatg tactttcagg tgctaagttc aatttagaag atgcatttgg
    17281 aaatatagta gttaaggatt tagttagtga tgaagatggt gtaattcaat ctagtgtaaa
    17341 aaaaccagga atatattatt tagtagaagt gatggctcca agtggatatg aaaaactgaa
    17401 ggataaaatt aaggttgaaa ttggtaataa agatattggt agaatagtag atatcggaaa
    17461 tatagaaaac ataagacaag aaaatccacc agtaaatcca cctgtaccgc caaatacaga
    17521 tgaaccaata gtaaatccac cagtgccacc aagcacagat aaaccaagga aaccatcaaa
    17581 tccatctgat acagaggata ctatagttat aaatccacca gtaccaccgt cagaagatat
    17641 aataaatcct ccaataccag agatattgaa tccaccagta ccaccatcag aagaggtgat
    17701 agaaactcct gttaaacaga ttgttccaat accagaagtg gtaaagccat cagtgtcaga
    17761 agaaaaaaat aataaagcta aagatgatac tttggtaaat ccaccagtac caccaaaaac
    17821 aggggatagt actacaatta ttggagaaat acttttagtt ataggagcta ttgtaggact
    17881 tgtcgtatta agaagaaata aaaatacaaa ttagacccta acattataaa aaatataagt
    17941 tatattaaaa aatatcttct aaaacaatat ttaataatga aaatattgtt agaagatatt
    18001 tttttttgaa gatttatgta aaaaaataat aactttttag aaaaaagttc caaagggata
    18061 tagacaatta aaaagatata agctataata tagatataaa attaaatgag gcaaaactag
    18121 ggaaacctag tcacgcaaag ctataggggc taaagtttag taaaccatgc cagccagttg
    18181 ccgaaaagag taaaaatttc tttttgtata tacaaagata ctcttgggta tcttttttat
    18241 tttgaaattt attactcccg gcctaactca aaggagggaa taatatgaga ccaagtaaaa
    18301 aattattaat agctataata tcaatattct taataagttc agttccagta agtgcacatg
    18361 catatagtac tactatacaa caaaataaag acacacttag tcaaatagta gtttttccaa
    18421 ctggaaatta caataaaaac gaagctaatg ctatggtaaa tagattagct aatatagatg
    18481 gaaagtattt aaatgtatta aagcaagata acttaaaaat aaaattatta agtggaaagt
    18541 taacagatga aaaagaatat gcttatttaa aaggtgtagt tcctaaggga tgggaaggta
    18601 ctggaaaaac ttgggatgat gttccaggct taggtggaag tacagtagct cttagaatag
    18661 gatttagtaa caaaggtaaa gggcatgatg caataaattt agaattacat gaaacagcac
    18721 atgcaataga ccatatagta ttaaatgata tatctaaatc agcacaattc aaacaaatat
    18781 ttgctaaaga aggtcgttct ttaggtaatg taaactactt aggtgtatat ccagaagaat
    18841 tttttgcaga agcatttgca tattactatt taaatcaaga tacaaatagt aagttaaaat
    18901 cagcttgtcc acaaacttat agctttttgc aaaacctagc taaatagagc tttaataaat
    18961 ttaaaataaa tataaaagag atgctattat aatagcatct cttttatatt gcgtttaaat
    19021 ctaataaatt ttgtcttatg tataacttga tagattatca attactactc cttatacaag
    19081 gcttcaacgt gtttattatg catttgagta attatcgtag gtataataga aattattgaa
    19141 ccaattacta tcaataaaat tcctataacc atgtttatag ggataatttc tttaagtatt
    19201 aataatgcta gtattggaga tagtactggt ttaaagaaga atactaaaga tgtaagacta
    19261 gtagatgtag cttccattgc cataaagtaa agtgcataac caacacctgt aacaaatacg
    19321 aatacataaa tcataatggg catattgtgt aaggtatatc cactaaaaat agggatattt
    19381 gcaaatatat gaagtttatt tttatttaat atatctgcga taaaattaat tttagtgagt
    19441 aaaaccaata tcaacatttc caaactacca aatataaagc taaagcaagt tacaactatt
    19501 ccaccaaatt ttaaggtttt tcttttacca aatacagtat acattgaaaa aataatagca
    19561 gaagataatg ttaagaatat tccactaaga gttagttttg tatgcaatgg atttattata
    19621 actataattc caagaacttc taatataagt gaaattacat tgtgcctata tattttttcc
    19681 ttcaaaatta agtaagctaa aagcattaca aagactggat tacaactgaa caaaacagca
    19741 actacagatg cctttgtgtt tagaacagcc agttgataca atgacatact tactactaca
    19801 cacataaaac ctaaaaatgc aaagtatttt aagtctgatt ttgtaataga aagactaaga
    19861 gctcttaatc tttttacagc aaaaggtagt aagactaaac cgccaacaaa aaatcttgat
    19921 aaagttattt gtaagggatt aaagtcatta gtgacagttt ttagactaat ttccattgaa
    19981 ctaaaaaata aggtagtcag tattatataa acataacctt ttttcaagtc aaacacctcc
    20041 ttgtaattta tattagtact atagataaga atattcaagc atattggaat aatatggtat
    20101 gtataaaata tagtacataa aattataaac taccttaata tagaatgtaa gtattctata
    20161 ctaaggtagt taattatttg aattactttg atttagttat tttaaattat ataatttggc
    20221 taatttttca aatgctggta aagaactttt aactttatca taagatacac aatatgatac
    20281 tctaaaatga cctggacaac caaaagaaga acctggtact aataataaat taaattgttt
    20341 tgcatcattg cagaattttt tatcatcttc aattggagct tttgggaaca aatagaatgt
    20401 accattaggt ttaacacaag aatatcccat atcgattaaa ctattatata gaaggtcaaa
    20461 attcttttta taaatattta catcaacttc tgcatctaaa cttcttgcaa taaccctttg
    20521 aaaaagagaa ggagcattta caaatccaag tattctattt gcaacattta atgaagacat
    20581 aacatcatca aaatcatcca tttgcccatt agcaacaata tatcctatac gttcaccagg
    20641 tagagataat gatttactat aagagtaccc tataaatgaa tttctatagt attttagtac
    20701 acaaggaact ttggcaccat cataaattat ttctctatat ggctcatcag aaattagata
    20761 tatgcttgta ttaaattctt tttgttttga atccaataat tctccaagat tttttaataa
    20821 ttcttcagaa tatatgacac cagtaggatt atttggagtg tttattatta aagctcttgt
    20881 ttttggagta atagcatttt ttagagcatc taaatctggt tcaaaagtct ctatatttgt
    20941 aggaacttct attaattttc catcatagtt ttctgtataa tttttatact caccaaaata
    21001 cggtgcaaaa gcaataactt catcaccagg atttaaaaga gtttttaaaa taatatttag
    21061 accaccagca gcaccacaag tcataattaa attttctctt gtaagattta atccatcttt
    21121 tttatttata tgttcagcaa tagcatctct tacatcctca tatcctgagt tgttcatata
    21181 tccatgaact aaatttggag attcttcatt taaaatatca tttataactg ctttaatagt
    21241 ctctggaggt tcaacacttg gatttcctat actaaaatca aaaacatttt cctcaccata
    21301 aatatcagaa agtttctttc cttcttcaaa catagctctt ataacagaac tatttgcaac
    21361 taaagtttgc attttattag atatcatatg taacactcct ttttatagat ttatattgct
    21421 tatgtatata ttaccatatt tttactgaat ttagacaatt ttcgaaaaat tacttgtttg
    21481 aaaatataaa cttttatata aaatatattt atgtacagtg catattaaaa attccaattt
    21541 ctaat
//
LOCUS       DAMJVX010000052        21501 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMJVX010000052 DAMJVX010000000
VERSION     DAMJVX010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.054"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            90..395
                     /locus_tag="PC407_001751"
     CDS             90..395
                     /locus_tag="PC407_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497201.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            803..1492
                     /locus_tag="PC407_001752"
     CDS             803..1492
                     /locus_tag="PC407_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2497202.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYFVKTAVDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNTIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            1492..2409
                     /locus_tag="PC407_001753"
     CDS             1492..2409
                     /locus_tag="PC407_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2497203.1"
                     /translation="MELFLVVISIVLFAFYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATLEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            2482..3408
                     /locus_tag="PC407_001754"
     CDS             2482..3408
                     /locus_tag="PC407_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2497204.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNDVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFSKKVAETDFEILKKIGTIIENPYFYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     KNQIGINSIGRKQSSLEDYFFQTTGTGGIETL"
     gene            3405..4085
                     /locus_tag="PC407_001755"
     CDS             3405..4085
                     /locus_tag="PC407_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2497205.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSILGILLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLVFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            4530..5231
                     /locus_tag="PC407_001756"
     CDS             4530..5231
                     /locus_tag="PC407_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2497206.1"
                     /translation="MNNSFLDKLIETKELKNSLYNVLKHNFLYHSNKIEGSTFTTEAL
                     ALLLDKNVVTGRHTLDDVQETVNSSYVFDTVIDSLKEKITHNFLRNLHSSLIFNTTLH
                     SRGMAGIYKTIPNMILGTDVSIAQPFEVEPKLDELIEWYYSQSEVSIKVIAEFHYRFE
                     LIHPFQDGNGRIGRFVMLKQMLENNLPIKIVSWDSEDLYRNSLNSCSLGNYVPLIEYL
                     SSLEDFREVYKMLWK"
     gene            6135..6785
                     /locus_tag="PC407_001757"
     CDS             6135..6785
                     /locus_tag="PC407_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2497207.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            7063..9129
                     /locus_tag="PC407_001758"
     CDS             7063..9129
                     /locus_tag="PC407_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2497208.1"
                     /translation="MKEVVESLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYVWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYIYDSNINKLKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNICILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSSMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            9146..11101
                     /locus_tag="PC407_001759"
     CDS             9146..11101
                     /locus_tag="PC407_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2497209.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKEAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDR
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLERSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            11117..12307
                     /locus_tag="PC407_001760"
     CDS             11117..12307
                     /locus_tag="PC407_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2497210.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(13155..13338)
                     /locus_tag="PC407_001761"
                     /pseudo
     CDS             complement(13155..13338)
                     /locus_tag="PC407_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427897.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13441..13815
                     /locus_tag="PC407_001762"
     CDS             13441..13815
                     /locus_tag="PC407_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2497211.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFVSTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMGILYEED"
     gene            13817..14674
                     /locus_tag="PC407_001763"
     CDS             13817..14674
                     /locus_tag="PC407_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2497212.1"
                     /translation="MENILEVYNLNKTYTNFSLSNISFVLPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMSEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTEYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            14677..15315
                     /locus_tag="PC407_001764"
     CDS             14677..15315
                     /locus_tag="PC407_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2497213.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVMIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            16289..17023
                     /locus_tag="PC407_001765"
     CDS             16289..17023
                     /locus_tag="PC407_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2497214.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEVSYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMVLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELTPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRDIIGTLKNLIIE"
     gene            18165..19421
                     /locus_tag="PC407_001766"
     CDS             18165..19421
                     /locus_tag="PC407_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2497215.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFLLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGIAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKTLGLEKFS
                     FALSSKVVFDLPIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            19484..20677
                     /locus_tag="PC407_001767"
     CDS             19484..20677
                     /locus_tag="PC407_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2497216.1"
                     /translation="MLTSLALIFLLGMASGGIFEKIKLPSLLGMLLTGIILGSYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMNIGIFLQIKYRVVARRLSVKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGIASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     EGKSK"
     gene            20884..21264
                     /locus_tag="PC407_001768"
     CDS             20884..21264
                     /locus_tag="PC407_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2497217.1"
                     /translation="MYLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKILSKLSKKRFLYVQETGKCMYYTVAIKEKTYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYI"
BASE COUNT         8040 a         2343 c         3657 g         7461 t
ORIGIN      
        1 caaaaacatg taatcgcata tataatataa ttataaaaat aataaatatt tttatagtag
       61 tagttaatta ttaataagga ggggtcaaaa tgcctagcat gattatggaa gttgtaggag
      121 tactatttat agctattgga ttaatcttga cattcattga aataaaagga aatttccctt
      181 ctttttatgg ttttggtcaa aattctatag ggaaaaatgc tgtaggtatt atagcagcag
      241 gaattgcttt aattttcttt agaaatggca tagatagcat agattctata gctacttatg
      301 gtgtattttt agcaatagaa cttgttgtaa tagttgcata tttcttaata agaaaaaaat
      361 ttactaaaga agataaacat ttaagaacta attaactaaa ttatctacta taaaattgga
      421 aaattaaaaa caaataaaag tttgttttta attatttaaa atcccaattt gattattttt
      481 aaagaaacta tattctgtat aaaactagaa tgtggtttct tttttatgta taaaatattt
      541 tttagcaaaa gttaaaaggt gttttataat ataaaatagt taattgtaga tgttaataaa
      601 agaaaattat gtatatttca gataaagata agagagtata ctatatttta attatttttt
      661 caataaaact tattgaaaag aactttaact tagaagagta tcaccaaatt gcagatacaa
      721 tatcataagc agttatttat tgacaaccct ttttaataaa tatatcattt gaagtatact
      781 gacgaaaagg agttgaataa taatggacat cttattggtt gaagatgata agaatattcg
      841 agaaatggta tacgattttc tgattagtga gaattatttt gttaaaactg ccgttgacgg
      901 aaatacagct attgaaatat ttaaaaatta ttcatttgat attattttac tggacttgat
      961 gttaccaaag tctagtggtt ttgaagtatt aaaatatata cgaagtgtga gtgttgttcc
     1021 tgttattatt actactgcga aagatagtga cacagataaa atgctagggc ttaatcttgg
     1081 tgcagatgat tatattacaa agcctttttc aatcgtagaa ttgcttgcaa gaattaaagc
     1141 taatattaga cgtgcaacgg tgtatagcag ttcaagaaac tctgaagata tggttattca
     1201 gtataaagat ttgattataa acatatcaaa atatgtagtt tcaaagaatg gaattgactt
     1261 aaacttaaca catacagaat ttgaaattgt taaattacta gcgactaatc aaggacgtgc
     1321 tttttcaaag gaacaattat acaacactat ttggaaagaa ccttattatg gaaatgaaaa
     1381 tgtgctaaat actcatatta acaggttaag aaataaattg tctgatggta tagaaaaaaa
     1441 taattatatt aaaactcttt ggggaattgg ctacaaaatg gaggaagaat aatggagtta
     1501 tttttagttg taatttcgat tgtactattt gctttctact taaaagaacg aaataagtca
     1561 aagaaaatgg aaagtgatta taagtatatc aattcaagac ttcgtgatat aacaaatgat
     1621 gatgaaatta attatatttt agtaccatct gatatttcga ttgtaaaaga aacagcagta
     1681 gatataaata aacttcttga aaagttttac agtaggcaaa ttaattataa tagaagtgaa
     1741 aaagcgatat tgcaaatatt tacaaacata tcacacgatt tacgaactcc catcacagta
     1801 ttaaagggtt atattgaaat gctatatctt caaagtcaaa aagaagaatt atcttcttca
     1861 atgagagcaa ctttggaaaa aatgcaaaca aattcaaatg agcttgttaa ttcaataaat
     1921 aacctgttta atatggcaaa aatccaatct ggagatatga tattaaatct tcaaaaatcc
     1981 aatctaacac aattatgtca tgagattatt ttagagtttt atgatttact agataaagaa
     2041 aactttaatg ttgaagttaa tattgaagat agacctatat atgcaaatgt tgacacagaa
     2101 gcattacaac gtatactcaa aaatttaatt gataatgcta taaaatatgg ttcagatggt
     2161 agatttttag ggatttcact atatgaaaag gaaaaacatg tttttataga tattgaggat
     2221 aaaggttcag gtatttctga aactgaaaaa gagcatattt tcacaagagc ttatactgct
     2281 gacagaaaaa aaggcaatgg tttaggtttg gctatttctc aagggcttgc aaactctatg
     2341 ggaggttcaa tttatgtaaa tagtagacct aatgtaaaaa cagtttttac ggtaaaattg
     2401 aaaagttaga aaaaagttag atttaaaaga gagaaaagat aattttattt tgtatactgt
     2461 ttttagaaag gagggagtat gatgaactat atcatagaga cccatcaatt aaagaaaata
     2521 tataaagata aagctgttgt taatgatgtt aatattcatg tgaaaaaggg agaaatttat
     2581 ggatttgttg gaccaaatgg agcaggtaaa agtacagtaa tgaaaatgtt gctaaatctc
     2641 gttaagccaa attctggaga aatagttatg ttcagtaaga aagtggcaga aactgatttt
     2701 gaaatattaa aaaagattgg tactattatt gaaaatcctt atttctatga gaatttgact
     2761 gctaaacaga atcttgattt acactgtgaa tatatgggtt attataacaa agaacatata
     2821 tcagaggtat tggaatgtgt cggattgtca aagcaatcaa acaagaaagt ttcaaaatat
     2881 tctttaggga tgaaacaacg tcttgcaata gctagagcaa ttcttactaa accagaatta
     2941 ttgattcttg atgaacctat aaatgcactc gacccagagg ggataagaga aatgcgagaa
     3001 ttattccgta agttaaatac agattatgga ataactatat ttatttctag tcatattctt
     3061 tcagaggtag aacaaattgc ggacactatt ggaattattc aagatggtaa acttattaaa
     3121 gaaatttcta tgtcagatat acacaaatat caaacagatt atgttgaagt agatgtagat
     3181 aatgtggaac ttgctggata cttgttagaa aaagaatttg gaataaaaga ctttaaaatt
     3241 acttcggaaa gttgtattga aatatatgat ttacgaaaag atgtaaaaga aatatccaaa
     3301 atatttatca aaaatcaaat tggtataaat agcattggac gtaaacaaag ttctttagaa
     3361 gattacttct tccaaactac aggtacagga ggtatagaaa cactatgata catttaatga
     3421 aactagaatt aaaaaaagtt gggctaaaaa gatatttttt attttcaata ctggggattt
     3481 tgttatgtat gttttttgtc tttactggat tgaatgataa atctacttca atatatgatt
     3541 atgatgttac atttaggaca atcagtttag ttttctgttt ttactatatt atattatttg
     3601 ctgtgttgaa tgtggcatat ataataaatg aatatacaaa taaaacaatc ttgattttat
     3661 tctcttatcc acttgaccgt aaaaaactta ttttatcaaa gttacttttg attacgactc
     3721 ttatgatatc atcaatgata gttggatata tctgttgttt tacattcgtt gttgtgttgg
     3781 ataagtattt taatttagtt attggagatt ttcatatttc aattttatct tattggatac
     3841 caggagcaat taaaagtatt attgtatttt gctcattagg tatatggaca ttcgttgttg
     3901 gaatgataag aaaatcaata cctgttaccc ttgtcagttc aattattttt atcaatttaa
     3961 gacaatttat tttagctggt acaaatacaa ctcaagacag tctgatttct gtttttatta
     4021 tagttggaat aactgtgatt ggtatatatt atatactaaa tcataaagtt actgaaattg
     4081 actagatact gtggatagga aggtagttat taattgtatc ttcttgggat aataatgatt
     4141 tgctattata tcagctatta ttaatattat tagagtttta ataatattaa taatagctat
     4201 atattttttc ttcttattta taaatttaat gatttatagt cataaaatga actctaagaa
     4261 gagataaaaa tgcgttttat atgtttttag attaatatga aatatcacaa tgaaatgttg
     4321 tacaaagtga tatataataa ttatatgaac tataaaattg actaaactta ttgactaata
     4381 aattagatga aaagttgaga cttactgaaa ataaatacaa tgaaatatat actacaagta
     4441 ttaaattaaa tctgaacttt acatactatt agaataagta ttataagatt ataatgattt
     4501 aaaaatatta aaataattga ggtgataaca tgaacaattc ctttttagat aaattgattg
     4561 agacaaaaga acttaaaaat tcactttata atgtgttaaa acacaatttt ctttatcatt
     4621 ctaataagat agagggcagt acttttacta ctgaagcatt agcactttta ctcgataaga
     4681 atgttgttac aggaagacat actttagatg atgtacaaga aactgttaat tctagttatg
     4741 tatttgatac agtaattgac tcactgaaag agaaaataac acataatttc ttaaggaatc
     4801 tacattcaag tttaatcttc aatacaaccc tacattccag agggatggct ggtatatata
     4861 aaactatacc caatatgata ctaggaactg atgtaagcat agcacaacca tttgaagttg
     4921 agccaaagct tgatgaatta attgaatggt actatagtca gagtgaagtg tctattaaag
     4981 ttatagctga atttcattat agatttgaat tgatacatcc atttcaagat ggtaatggtc
     5041 gcataggtag atttgttatg ctaaaacaga tgttagaaaa taatcttcct ataaaaattg
     5101 tatcttggga tagtgaggac ttgtatagaa actccttaaa ttcttgtagt ttaggtaatt
     5161 atgtcccttt aattgaatat ttatcaagtt tagaagattt tagagaagta tataaaatgt
     5221 tatggaaata attgtttgta gcaaaaaaag tttttatata aatatatttt taactatgct
     5281 atttaacgtt gatttcttag ttattcttag cttggactat acccactatt ttaactatat
     5341 aaaatagtgg atatagttta actatctctt tataactatt tttatgtatc ttagttgttt
     5401 cttaatattg ggacagttca taaaactaaa agtgattttt aattttatca tttataattt
     5461 accattacat cagaaaaatg cttatcatta gaattttcat aaaaggtacc tattttcttc
     5521 aattaactaa atcaattcta ttagtcttaa tctttcgtat ttcaaattga taatacaaga
     5581 aaataaaaaa taaaaaattt taaatttttt atttattgcg aaaaaaataa aaaggtgcta
     5641 tactttttgt aacatattat tcaaattatt aagagatagt aaatatttat agtttaattt
     5701 gtagtacact agcatgattt gcagaacaat gaattaatct atagtataat agtttaattt
     5761 gaagtacaac gatttaatct atagtacact agcatgattt gaagtataac gatttaatct
     5821 atagtacaat aacttgattt gcaatacaat aatttaatct atagtataat agcatgattt
     5881 gaagtataac gatttaatct atagtacaat aacttgattt gaagtacaac gatttaatct
     5941 atagtacaat aacttgattt gcaatacaat gatttaatct atagtataat agcatgattt
     6001 acagtacaat aatttaaatt gtagtacaaa agtactatca aataagaatt tctactttaa
     6061 aaatattact aaaagtaatt aaaatccaat aattcaatat aatatggcaa aagaatagaa
     6121 ataaggtgag agtagtgaaa gtaaagtcaa atttagatga aattttatat cataagatta
     6181 ttgaaagtct tattcgtggt gaatactctg taggtcaaaa aatattacta aatgaccttt
     6241 gtgaaaaatt tgaagtaagc agaacaccag ttgtacaagc agtaaaaatg ctaaataaag
     6301 atggagtgct tactattatg acaaatggta aagtgtacgt accagagtat gaatatgata
     6361 tggtaaaaca agtttgtgaa acgagaacac tgattgaaac atatgcttta gaaaaaatga
     6421 tgcaagaaga ggaagaggtt tttcaacaaa agctagatgt aataaaaaga tactctgata
     6481 aatgcgagga gtactatcgt caagaaaaat ctgtagaact tgcactagca gatttggaat
     6541 tacataaagc cattgtagaa ggggcaaata atgagatttt aaatgatgta tatgttggta
     6601 tacaaggaag atttatagta gtgaactatt taattcgacc attaaaaaat cgtaattatg
     6661 aaggaacagt acaagaccat tttgaaatat taaaattcat tgaagacaga gatacaaaaa
     6721 aagcagtaga taaactaaga aatcatattc aaggaactat taaaagattc tgtgaagatg
     6781 aataataagt tttgaagtta ctatatactt atctattaag ttgctaaatg tagtctaagt
     6841 ataatctaat tatagtttaa acataatcta aatataatct aaatatagtg taaataagtc
     6901 taagtatgaa ttaaatatag attaagtatg aattaagcat ggattaaata tactttaaat
     6961 tacaaatatt acaaggaagc cagaaggctt cctaaaaaaa taacctttat aaaatttttt
     7021 attttaaatt taaaattttt tattttaaat ttaaaataaa aaatgaaaga ggtggtagag
     7081 agtttgaaaa aaattactaa agaaaggata gaaaatacta tgaaaaagct atttacgagc
     7141 gaaacatttt tggaatatca atttataagt gattgccaaa tatcaccaga tggaggatat
     7201 acagcattta ttgtaaaaaa agctgatatc aaagagaatg gttacacaag ccaagtttat
     7261 atattaaaca ataaaactgg cgaattaaaa caaataacgt ctataaactc tgttggagca
     7321 tatgtctggg aagatgaaaa tactatcttg ttccctgctt tgcgaaatga aaaagtaaaa
     7381 gaagctgtca aaaacggtaa gcaatgcatg agttactatg ctttatcttt aaatggtgga
     7441 gaagcagaag aactttttag actccctatc aaaggtggta aattgaatcc tataggaaaa
     7501 ggtttgtatg cagttattga ttcttatgac aatgaccgac ctattgtaga gggattgcca
     7561 gaagaagaac aacagaaaaa aataaatgct tataacaaaa gacattatga acatttcaaa
     7621 gaaattcctt atgctgtcaa tggggaggga tatattagca gaaaaagaaa acgcctatat
     7681 atatacgaca gtaatataaa taagttgaag gcaattacag cacctatgtt taatgttgta
     7741 ggtatgaaaa taagtgatgg aaaaatctta tatatagggc aagagtttaa agatgtaaaa
     7801 ggtttgaaaa atggggtcta tgtatttgat acaaacacaa acacaaatat ctgtatatta
     7861 gaaaaagata agtatattat aaaaggtttt gaattatatc aaaatcaagt gatactaaac
     7921 ctaactgatg ctttatctta tggaaatgga gaaaatggag atttttatac tattgatatt
     7981 gatacgaaag aaatgaaact tctttcaggg catcaacatc attgtattgg aaatacagta
     8041 acatctgatg ttaaaatggg tgctggtcag acaacaaagg tagatggaga atatatttat
     8101 tacacttcta cagtagatat ggactgcatt attgaaagaa tacatattcc aacaggaaaa
     8161 caggaaaaag taacacagac aggttcagtt gattttatag atgtaaaaga tggcaatatt
     8221 gtatgtgtgg gttgtttagg aaatggatta ccagaagttt atacagtaga gaatggaaca
     8281 ttgtgtaaga aaacacattt aaatgaccat attttagagg aatataaaat ttctgtgcca
     8341 gaatacatag agtcaaaagg aagttctaaa tgggaaatac aaggatacgt atataaacca
     8401 gtagattatg aggtggggaa aaaatatcca gctgtattgg cgattcatgg aggaccaaga
     8461 ttgacatatg gaccatattt tatgcatgag atacaggtat ttacaagtgc aggttacttt
     8521 gtattcttct gtaacccaag ggggtctgaa ggtcgtggca atgcttttgc agatattagg
     8581 aaacagtttg gagatattga ttatatagat tttatggaat ttacagatac tgtacttgaa
     8641 aaatatacag acattgataa aacaaaattg gctgttgaag gtggaagtta tggtggattt
     8701 atgacgaatt ggataattgg tcatacaaac cgttttgctg ttgcttgtgc tcagcgttct
     8761 attgcaaact ggagtagcat ggagggtaca acagacattg gttattattt ctgcaaagga
     8821 caaacaggtg cttctcatat ggaaaaccac gagcttcaat ggaaacagtc acctcttgct
     8881 tatgctgata aatgtgttac accgacttta tttttacatg gtgaaaaaga ctatagatgc
     8941 tatatgcaag aagcattcca gatgttttca gcattgaaaa ttcatgggtg tcctacaaaa
     9001 ctttgtctat ttgaagggga aaaccatgaa ctgtctcgtt ctggtagacc aaaacaaaag
     9061 ctacaaagat tagttgaaat gctagattgg ttttctgtgt acataaaaaa agataacata
     9121 ggacagtaga aaggagaata taaaaatgga aaaaataaag attgaaagtt cagatatttt
     9181 tgaatatata ttccctcatg atatttcttg ttcacctgat ggaaagcata ttgcttatat
     9241 tatatcaaat atcaacgagg aaaaagactg ctatgaacat gacttatatg tgatggacat
     9301 caaaacagaa aaacaaatac atatgactca aacaaaagat gtaacatcat ttagttggat
     9361 ttctaataca gaactacttt ttacatctaa aagaaataag ccaaaagcag gtacaacaga
     9421 tttttacact atttctatag aaggaggaga agcaaaagaa gcattttcta ttccaaaagc
     9481 ttgtagcgta cctgtatcac ttggaaataa attgtggtta ttgacaacaa aaaatccaac
     9541 ggattctaaa aaatcagaac cagatagggc agtagagggt gtagattatt ggacatttac
     9601 agataaacct tttataagag atagagaaaa tttttctcaa agaagacgtg taacattaga
     9661 attatatcaa gaaggtgaaa atataacaaa agccattaca ccaaaatttt gtgaagtagc
     9721 aggtatagac gtttcttctg ataaaaacag aattttatat actggtcaga tttatgaaga
     9781 ttgtgcaact cctttttctg gactttggga gtatcatata gacagtggtg aaaccaaaga
     9841 acttgtacca caaggaaagt accaaataag tttggcaaaa tatattggca aagataaagt
     9901 aatgttgcaa gcttcaacat tagaacgttc tatcacacag aatcatgata tttttatact
     9961 tgatttgagt actagtgaaa taaatatgat tgctagtcca gatggtatgt atgctacatt
    10021 actggatgta gatgcagtat atggtggtgg acgtagtaat aaagttatag gagataaatt
    10081 tattggtgca agaatttgca gaactatgac agaatttaat gagtttgata caaagacagg
    10141 taatatacgt atcatcacaa aagtagatgc ttttacatct tttgatattt acgacaacac
    10201 catgtacaca gtaatgttga aagattatga gttagcagaa atatatagca ttgatatgac
    10261 tacaggtaca atgaaaaaaa tgactgcttt ttctaaacca tatttagata ctcataaggt
    10321 ttcattacct gaaaaattga cgtttgtagc aaaaaataaa gaagaagttg atggttttgt
    10381 tataccacct atagatgcta aagaaggcga aaaatatcct gctgtattgt tcattcatgg
    10441 tggacctaaa tgggcatatg gctatatgtt tacacacttg aagcagtgtg taacatcaaa
    10501 gggaatgtat gtgatttatt gtaaccctca tggtggtgac ggttatggtg aaaaattctt
    10561 agaaatggta gaaagatggg ggtatgttga ctatgagcat cttatggaat ttgtagatac
    10621 ttgtattgaa aaatatcctg ggattgatgc agaccgctta ggggttgcag ggggaagtta
    10681 cggtggttat atgacaaact ggataattgg ccatacagac cgtttcaaag cagcagtttc
    10741 tcaaagaggt attagtaact tgattacagc ttctttaatt attgactttg gggatagaat
    10801 tatgaagcag acttgtggag ataagacgcc ttggaatcat gaggaggttc tttggaatca
    10861 ttcacctatt aaatatgtga aaaatgttaa gacaccaaca ttgttcttac atagtgatag
    10921 ggattataga tgttttatgg gggatacttt ccaaatgttt acagcattaa aacagcttgg
    10981 tgtagataca gaaatgtatt tgttccatgg agatacacat ggattgtcta gaaatggaag
    11041 accatctaat agaattgcac gagcaaatgc tattgtagat tggtttgaaa gatatttata
    11101 ataagggggt tattctatga gtgatatgtt attatggttt agtgtcgtat tagttttatt
    11161 tgctattggg gatttaattg caagcaaaac aaaagcaaaa gtatcagctg tatttgttac
    11221 actactttta tttttgattc tatttgtcac aaaagcaata ccagctgata ttattgaaaa
    11281 agcaggtatg accgcagcag ctagttggag tgttccaatg attatgttta gtatgggaac
    11341 aatgttgaat gttaaacaat ttatagacga atggagaaca gtattgacag catggcttgg
    11401 aattgtagca gttatagtct gtgtctcatt atgtattcct ttgtttggaa aatcaacagt
    11461 attgacaagc ataccagtta taaatggagc tttacctgct actactatta tgacacaagc
    11521 agcactagaa aaaggactga cacttgcagc agctacagca acagttgtat ttgcaattca
    11581 gaagtttatt ggtacaccaa tagcttcaag agcagcgtta caagaggcaa accgtctttt
    11641 agtagaatat catgaagcaa aatcaaaagg cattgattta gcaaatgttg atactgataa
    11701 gaaagaagca gagaatacag gcacaaaagt aaaacaagct ttctgtgaaa aatacgataa
    11761 atattattct acaaatgttt gtatattttt tatagcacta ttctcttatc tagggtatga
    11821 actttcagag attattcatg taaattattc tattgtatgt cttgttgttg gtgttattgt
    11881 aacacgtatt ggtattgtac caaaagacat tttggaaaaa ggaaaaatca agggatttat
    11941 taacatggtt gtgtttgcag ctgttattcc ttctttagca aaagtttctt tgacagattt
    12001 aattagtctt tttgtaccaa ttgtgggtat gtttgcagct tctatcattg gtatattcct
    12061 tatgatgaag gtgttaccag gttggaaaat tatcggttct aaacctcttg cttttggagt
    12121 agggttttgt cagatgttgg gattcccaac tacatattta atttcaaatg aagtgtgtaa
    12181 tgctgttgga gaaacggaag aagaaagagc atatttgatg tctaagatta tgcctaagct
    12241 ggtagttggt ggtatggctt gtatgatttc tattgtagta gctggtctta tggtaccaat
    12301 gttgtagata ggtttcatag ttaaaaagtg gtgttaattt ataggagaag ctaaaaagta
    12361 gtattaattt ataaaataat tttatattag ttaatttgtt ataagaatat tttcttttta
    12421 tgattataat tttgatttta taagagagca gattttttat ttgctatgct aatagttata
    12481 atttgttttt atttgctaaa atgcaacttt aatttgattt atgtataata tagggtattt
    12541 aataatattt ttgaagaatt tttataaaaa attaaaaaga ggagaaaaaa gggagtgcca
    12601 caaagtgaac tttttagttc taaggcactc ttttttgtac gaaatcgaat atattttcaa
    12661 cgttttaaaa gtgagaaaga gacttatctc tagtttggga tagccaaatt taattattaa
    12721 aattattttt tgttttattt atcaaagagt attcgtgttt tagaagttag tattttttat
    12781 tttatatatc caaggttttc cgtttctaat gttttctttt ttaaaataat tagggtattc
    12841 aattatagta aaaggtggat ggtattttgg aatgatatta tttaaagcac agtattgaat
    12901 gttgaaatcc tcacatttta gagtattatc ttttgtaatc aaaaaaatct tctatactta
    12961 tagcaaggtt acttctgcaa atattccatg catcataata tccaaattta aattttttta
    13021 atatagagtt tacctctagt ctacactctt gaataactct ttcctctatc caaattctta
    13081 taaactcatc ccatttatta ctatctattt attactatct aaatttttta gtatgtttgt
    13141 aattggaaat ggatttattc ctttatgttt tttataaata gtttataact taatttatta
    13201 ttaaacaaat cattcataca gaattacttt ttcttttaga tattcaattc ttacttttaa
    13261 taaatattta atacattcta tcttatgtgg ttttaaaaag tgtttatggc tatcaataat
    13321 ctctaatatt tcattcatat ttattttgtc atagtaatat tttacatctt ttatattgac
    13381 aaaaaagtgc tctaagtgta tagtgtgtat ataataacat cacactggag gtgataaaaa
    13441 atgaaagtgc tcatttctaa cacatcagat aagccattat accaacaaat taaagagcaa
    13501 attaaagaag caatatttac tgaagaactg aaagaagggg atgcaatgcc ctcaataaga
    13561 aattttgcta atgatttaaa agttagtatt ttgactatta gacgagtgta tgaagaactt
    13621 gaaaatgaag gttttcttac acgccaagtt ggaataggaa catttgtttc aactggaaat
    13681 ttagaaatac taagagactc aaagaggcgt atagttgaac aaaaaatggc agatatgatt
    13741 aaggatgcaa aagcactagg aattacacag atagagttac aagaaatgat gggtattcta
    13801 tatgaggagg attaatatgg aaaatatttt agaagtttat aacttaaata agacttatac
    13861 caatttttca ttatctaata taagctttgt attaccagaa gattgtataa ctggatttat
    13921 aggtgttaat ggagcaggaa aaacaacaac aattcgttcg atattggatg tcgtaaagcg
    13981 tgatagtgga aaaataaaaa tctttggtaa agatttcgat ggaaatgaga aggaattaaa
    14041 aaatcgaatt ggagttgtat ttgatgattc atatttctat gaggagttgt caatgtcaga
    14101 aatgaaaagt attattgcac cttcatatga taattggtgt gaagaagatt atcaacaaaa
    14161 tatgaagcgt tttaatctta atccaaaaca aaagattgat acactttcaa aaggaatgaa
    14221 aatgaaattt gctttagcat tagcattatc gcataaagct gagtttttaa ttatggatga
    14281 gcctacaagt ggtttagacc cactaatccg tagtcagata cttgacatac taacagaata
    14341 tatgaagaaa tgtggtcgtg gtgttttctt ttctactcat ataacatctg atttagacaa
    14401 aatagcagat atgattgtta tgattgacaa tggacgtatt atattagagc gtgaaaaaga
    14461 tactctattg gatgaacata gaaaagtaaa aggggatatt caatttctaa atgatgaaat
    14521 caaaaaatta tttttaaatt tagaaattac aggttatggt tttataggga ttacctcaaa
    14581 aattaatgaa gtgcaaaaaa atatgccaga tattttaata gagcgtccta caattgaaga
    14641 tattatgctt gcatatattg aaaggagaga gtaattatgt tgattcattt agttaaaaaa
    14701 gatattctga ttgtaaaaaa atatgtattg ttgatattgt ttattgcaat aggaatccca
    14761 ttatttatat tatggcgttt acctccattt gcacaaatat tgggatttgt actatcaatt
    14821 atattttcag aatttatgct gtgccagtat ctattattaa aggagagtca atattcaaaa
    14881 gcatctgtat tactttgttc aactccttat cctagacgtg gcttagttca atctaagtat
    14941 gttttatttg caatggtatt tatctattgt acattagttt actggattga aaatttactt
    15001 attccacaaa taggaacttt tgacttaaca tatattttga ttgttttatt aataagttct
    15061 attatttatg gagtttatat gcctattcaa tacaaattag gatatgaaac aacaaaattc
    15121 tttttcgtga tgataatcat ggcttcatca tttgtattac cattaactat agctcacagc
    15181 aattttgaaa ttccatttat atatcagtat ccatccatga tagtgaatat tgtattgttc
    15241 cttttaagtt taattatact gttagcttca atgtttattt ctattaaaat ttttagtaat
    15301 aaggagcttg tataaggtaa gggggaattt tatatgccac atttgccaga ttgggctgtt
    15361 ttactttgta tcttacttcc aatagtatat aaagcttgtc agatatttaa aagtaaaaaa
    15421 tagagtacat atttatattg ccatttttat tgagtgaagt tagatggtat gaatttaaaa
    15481 ggttacttga aaataaagtt acttggtgtg ggagagtaac atcataatat tatagattat
    15541 atgtttcttg tgaatcaatt tgtatttttt aatagataat atccagtaat tttattttcc
    15601 attgattatt ttcaattatg agtatgattg tgtgtattgt ataccatctt ttaaggtgtt
    15661 tttaaaaaat tatatcgtca aaatataaat ctaaggaatc ctataaataa agcttttcaa
    15721 ttttagaaaa ctaataaaaa gtaggaagtt aataaaaaga agctgtctca aaatagagat
    15781 aagcttcttt ttcattgctg aaatgacgaa aatataattg atttaatata aaaaagagtg
    15841 ctttatgaac tatttatttt gaacagtaga tacacttgca ctcaatttag ttactcctaa
    15901 gctttatgaa ctaaaagttt attttgaagc ctatttttct aaataatcat ctatatacag
    15961 tattgcgttt cttttattta ttcataattt attttcttat taaagttttt ctatattact
    16021 ttacttttaa ttttatacga attatcaaaa ataaaatagt tattagaaaa aaataaaagt
    16081 aattgcaaag attgcaatta cttacttcta tattaattac tataccataa aataacaaaa
    16141 aaatcaagtc gagtaatcct tcaacttaag tgatatacta ggtattaata atttttaatt
    16201 aataaaatat aactagagga aaatctacat tttctcaatt tctttaatta aattgacctc
    16261 taattaagta attataagga gaaaaattat gaatttagca aagggattag atgttttaaa
    16321 aatttcatca aatgtattcg gtgaagataa agttatgtat attcctgtaa tctacacaga
    16381 agatgatgca acacttattg acacaggtct tccgggtcaa ggtgatttaa taattgatgc
    16441 tcttaataaa agtaacacga gctttgatag gttaaaaaat ataataatta ctcatcatga
    16501 tatagaccat ataggaaaca taaattatct aagagaaaaa tctaagaata atatcaaagt
    16561 atatgcatac aaaagtgaag tcagttatat aacaggagaa gaaactcctt ttaaacttta
    16621 tatgttggaa caaatggtag ataaaattga tgataagatg cttagtatgc taaatgttat
    16681 ggtacttgga tttaaatcat catatactaa agtagatgtg tcattagata accatgaaaa
    16741 attgaatcta ggtgaggaaa tagaagttat acatacagga ggtcacacta gaggtcacat
    16801 atgtctttat ttaaaagagt ctaaagtact tattgctgga gatttgttac aggtagaaaa
    16861 tggagaactt acgcctgttg atgtaatgca cagcaacaaa caagagttaa aagatgctat
    16921 taaaaatata agtaattatg atatagaaac gatagttttt agtcatggtg gattatatca
    16981 aagagacata attggaacat tgaaaaacct aattattgag tagtactata tgatttacaa
    17041 taatttctta aattacttaa aattatatct aaagatgtag aatcaaaatt aaatataagt
    17101 aataagaatt ttgctttaaa attatactta agtgttaagt atgatacgta gtattacaag
    17161 tattttagaa tataattttt tatagtaatt ttaaggttct acaatacttc agttgaaaaa
    17221 tatttaacag catagtataa taggaaagaa aatattttaa ataaacttta gtatacaaag
    17281 tgaagaaaaa tatttaaatt atatataata ttgtagagtg aggtaaaata tttaagttat
    17341 atatcataat acagagtgaa gaaaaatatt tagataatgt accataatac aaggtgagta
    17401 aaaatattta aagcatgtat aataatgcaa agcgaagaaa aatatttaaa taatgtacca
    17461 taatgcagag tgagtaaaaa tatttaaata atgtgttata atacaaagcg aataagatta
    17521 tttaaagcat gtattataat ataaagtgag taaaaatatt taaatcatgt atcataatat
    17581 aagcagagtg aagaaaaata tttaaataat atatcataat gtagagtgag gtaaaatatt
    17641 taagttatgt atcataatac aaagtaaaga aaatcattta aattatatat cataaaaata
    17701 atatgaaata ttgaacactc ataatattgg aggctttaat attttaattg ataggttaca
    17761 ttatttataa cattaaaaat acaatttaca taaaatagac atatgaaaaa taaaaaatta
    17821 tataataatt atgggaatga ggttctccct tggatgataa tatcctaaac tgccaatgag
    17881 ctgatgactt ctatgatatc atagatgcca ccagcttttt ttgttgttta aatcgataaa
    17941 atggaggtga attacttact aaatttttat tttttaaatt tgaagaaata tttaattaat
    18001 agaaaaaaca gtagattata tgttaattat tttattaaga atgggttaag agtataccta
    18061 tatcctatca atttaaaaaa caattatgat agtcattaca ttgactacag ttgatgcaat
    18121 aaaactttca aaaaatctta tgatgcaaat agaaaggaat taaaatgata gaaaaattta
    18181 aaactttaat atggattaaa atgataataa gaattaaaag cgtttataaa acatatggtg
    18241 gactattttt gttaggactc ataggtatac cagttggtgc aattataggt cttatagaca
    18301 ccatatttgg aacggttctt ctaaaagtta cagatattcg agaaacatat ccaatgtatc
    18361 tgataccatt tttagcagta gtaggagttg ttattgctta ttgctatttt aaatttggag
    18421 gaaaaagtag taaaggaatg aatctaatat ttgaggtagg gcatggagaa gaagaaatta
    18481 ttcctttaag attggttcca tttataatat caggtacatg gttaacccat ttatttggtg
    18541 gtagtgcagg acgagaagga gttgctgttc agattggtgc aactttttca cattgggtag
    18601 gtaaaaggtt gcctataaaa aatgcttcta gtatttttct agtaactggt atagcagctg
    18661 gttttgcagg attatttgag acaccaatag cagcaatact atttgctatg gaggttttgg
    18721 tagctggttc actggagtat caatcgcttt ttcctgcgtt tactgcttct tttacagcaa
    18781 gtgcagtatc aaaaacttta gggttagaga aattttcttt tgcactttct tctaaagttg
    18841 tatttgactt accaatattt tggaagctta ttgttttagg aattattttt ggaatggttg
    18901 gtggagcatt tgcatggtgc ctgaaattgt caaaaagaaa aataggtaat agattaaaaa
    18961 atcctatgat aaggattgct attattggag tatgtcttag tgtattattc cttttatttt
    19021 ataaaggaag atattcaggt ttagggacaa atttaattca aaatagtttt tatggtggag
    19081 aaatttattc atttgactgg ctgttaaagt ttatacttac aatactgact ttgtcagcag
    19141 gttttcaagg aggagaggtt acacctttgt tttccattgg agctagtctt ggtgtattgt
    19201 tagcaggatt ttttaatttg ccaattgaat tagttgcagc gcttggctat gctagcgtct
    19261 ttggcagtgc tacaaatact ttttttgctc ctgtatttat aggggcagaa gtttttggat
    19321 acagctattt accttatttc tttgtagtat gcgctatttc atatatattt aatatggata
    19381 aatctattta ttcactacaa aaaatttcaa cgaaacaata aataaaacaa cataatgtat
    19441 gattataaga gaaagaatat tagagaaagg atagggtagg aatatgttga caagtttagc
    19501 tttgattttt ttattaggaa tggcctcggg aggtattttt gagaagataa aattacctag
    19561 cttattggga atgttactta caggaatcat attgggttca tatgttttaa atttgattga
    19621 taattcaata ctagatattt cttctgacct tagaaagatt gcattgatta ttatattaac
    19681 aagggctgga ttatctttag atataaacga tttgaagaaa gtgggaagac ctgctgtact
    19741 tatgtgcttt attccagcta cttttgaaat tatcggaatg atagttttag caccaaaatt
    19801 actaggcgtt tctatattgg aagcagcagt catgggtgca gtagtaggag cagtttctcc
    19861 cgcaattatt gtacctaaaa tgcttaaatt aatggaagaa ggttatggta cagaaaaaag
    19921 tattccacag atgcttttag caggaacttc tatagacgat attttcgtaa ttgtgatgtt
    19981 tacagtattc acaggattgg cacaaggaaa tagtatatca gctattagtt ttttacaaat
    20041 tccagtatca attatattag gtgtaatagt tggagcagtg attggaatct gtttagcagt
    20101 attttttaag aaggttcata tgagagattc tgctaaaggg gtgttgcttt taagtatctc
    20161 atttttaatg ataagtttgg agactgcttt agagggtatt gtgccatttt ctggattatt
    20221 agcagttatg aatattggga ttttccttca gataaaatat cgtgtagttg caagaagatt
    20281 gtctgtaaag tattcaaaac tatgggttgg tgctgaaatt ctcttgtttg ttttggtagg
    20341 ggcaacagta gatataagtt atgcatttaa agcaggaatt ggagctgtaa ttttaatatt
    20401 tggagttctt ttattccgta tggtaggagt attcttctgc cttattaaga caaatttgac
    20461 aataaaagaa agattgttct gtatgattgg ctatattcca aaggctacag tacaggcagc
    20521 aataggaggt gtaccacttg caatggggat agcatctgga caacttattt tgacattagc
    20581 ggttctcgct atacttataa ctgcacctct tggagctttt ggaattgatg taacttataa
    20641 aaaattatta acttctgtag aaggtaagag taagtaagaa taaaaataaa gattatttta
    20701 agtgataaat tatattttgg aaaaaactat attattgagt acaatgttag aaaatcattt
    20761 gagtgattac agaaggtaga agtttatatc ggattttaag gcatgtgatg attattagaa
    20821 ttgattttgt gaaattgtta ttgtaatatt taattatata gataaagaag cggagtgata
    20881 actatgtatt taaaaaaatt atcaaaatta gagttagtta ttatgaaatt tatttggaat
    20941 ttagatataa aaaccaattc atatgagatt attgattata tgaaagaaga gcataattta
    21001 ccagaaaaag tggctttaaa aattttatca aaattatcaa agaaaagatt cttatatgtt
    21061 caagaaacag gcaagtgtat gtattataca gttgcaataa aagaaaagac atatttagag
    21121 tttatatcaa gaaatgtgca aaatcttttg aagaataatt ttataaggaa tttattagta
    21181 tcatttcacg aagaggagct aacagaggaa aagataaagt cgttagaaaa ctgggtagta
    21241 aattgggaag aagcttatat ataaatgtta ttatttttat tacaatttag ataattttgg
    21301 ttttgtaagc tctgcaacta tttttagatg gttgcagagc ttatttttat tttttattcc
    21361 cttaagcaat acttaaaagt aaacgcattt atatttacta acttcaatca cttctaatac
    21421 ttagattact ttttaaaaat attttgatat gtcatctcta tagtaatcta gaacttgttt
    21481 ctattcttta tataaagttt t
//
LOCUS       DAMJVX010000053        21433 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMJVX010000053 DAMJVX010000000
VERSION     DAMJVX010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21433
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.055"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(41..919)
                     /locus_tag="PC407_001769"
     CDS             complement(41..919)
                     /locus_tag="PC407_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2497218.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            complement(936..1253)
                     /gene="trxA"
                     /locus_tag="PC407_001770"
     CDS             complement(936..1253)
                     /gene="trxA"
                     /locus_tag="PC407_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2497219.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            complement(1403..2635)
                     /locus_tag="PC407_001771"
     CDS             complement(1403..2635)
                     /locus_tag="PC407_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2497220.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            complement(2625..3296)
                     /locus_tag="PC407_001772"
     CDS             complement(2625..3296)
                     /locus_tag="PC407_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2497221.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            complement(3312..4148)
                     /locus_tag="PC407_001773"
     CDS             complement(3312..4148)
                     /locus_tag="PC407_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497222.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            complement(4174..4848)
                     /locus_tag="PC407_001774"
     CDS             complement(4174..4848)
                     /locus_tag="PC407_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2497223.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKRKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            complement(4862..5590)
                     /locus_tag="PC407_001775"
     CDS             complement(4862..5590)
                     /locus_tag="PC407_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2497224.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIKDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSGLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            complement(5717..6406)
                     /locus_tag="PC407_001776"
     CDS             complement(5717..6406)
                     /locus_tag="PC407_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2497225.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLRVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMIGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(6414..6977)
                     /locus_tag="PC407_001777"
     CDS             complement(6414..6977)
                     /locus_tag="PC407_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2497226.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(6979..7947)
                     /locus_tag="PC407_001778"
     CDS             complement(6979..7947)
                     /locus_tag="PC407_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDF2497227.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(8048..8091)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            8456..9085
                     /locus_tag="PC407_001779"
     CDS             8456..9085
                     /locus_tag="PC407_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2497228.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKAIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQSY"
     gene            9060..9464
                     /locus_tag="PC407_001780"
     CDS             9060..9464
                     /locus_tag="PC407_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDF2497229.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            9467..9940
                     /locus_tag="PC407_001781"
     CDS             9467..9940
                     /locus_tag="PC407_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2497230.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            10487..11152
                     /locus_tag="PC407_001782"
     CDS             10487..11152
                     /locus_tag="PC407_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDF2497231.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            11174..12106
                     /locus_tag="PC407_001783"
     CDS             11174..12106
                     /locus_tag="PC407_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDF2497232.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            12127..12768
                     /gene="pcp"
                     /locus_tag="PC407_001784"
     CDS             12127..12768
                     /gene="pcp"
                     /locus_tag="PC407_001784"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDF2497233.1"
                     /translation="MKILLTGFDPFGGELINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIK
                     GGFIHIPYTTSQVINKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(12887..13219)
                     /locus_tag="PC407_001785"
     CDS             complement(12887..13219)
                     /locus_tag="PC407_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2497234.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            13485..14105
                     /locus_tag="PC407_001786"
     CDS             13485..14105
                     /locus_tag="PC407_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2497235.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKEAI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(14430..15254)
                     /locus_tag="PC407_001787"
     CDS             complement(14430..15254)
                     /locus_tag="PC407_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HDF2497236.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     TLFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(15265..16668)
                     /locus_tag="PC407_001788"
     CDS             complement(15265..16668)
                     /locus_tag="PC407_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2497237.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIREYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(16672..17397)
                     /locus_tag="PC407_001789"
     CDS             complement(16672..17397)
                     /locus_tag="PC407_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2497238.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDVQNESKNLNKNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(17509..18282)
                     /locus_tag="PC407_001790"
     CDS             complement(17509..18282)
                     /locus_tag="PC407_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2497239.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFLGFLLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNEPIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVRYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(18288..19175)
                     /locus_tag="PC407_001791"
     CDS             complement(18288..19175)
                     /locus_tag="PC407_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2497240.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDANYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVFDEP
                     TAGIDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSGDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(19189..20211)
                     /locus_tag="PC407_001792"
     CDS             complement(19189..20211)
                     /locus_tag="PC407_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497241.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETERYYID
                     KEKGCIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            20786..20962
                     /locus_tag="PC407_001793"
     CDS             20786..20962
                     /locus_tag="PC407_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497242.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         7460 a         3378 c         2282 g         8313 t
ORIGIN      
        1 ttttttataa atatctttaa aaagttaatc aatataaata ttaagccctc tttaatttat
       61 ctaaatagta aacagcattt aaagctgcta tttgcccttg accagctgct tttatataag
      121 aatagggttt tccaacacaa tctccagcag caaagcatcc atctatacta gtcttcatat
      181 tattgtcaac tttaatatga atgccatcaa tctctattcc aggaaccaat gcacttggag
      241 ccgtactatc ttttataacg aatactccat caacttcaat gtggttttct ttaaaagata
      301 ctttgtttac aagtttatct ccatctatct gtactggtct gtcatgtata acttcaatag
      361 aatcatctag gttatctgaa cttctcataa gattatcttt tttgtacata ggtataaaat
      421 aagtctttga agtgagttca cttaaaaaat ttgcttcttc tttagattct tcattatatc
      481 ctataactgc taccttttta tttctataaa gtggtgcatc acatgtagca cagtagccta
      541 cacctcttcc tagaaattct tcttcaccct tgattggtct agtatactct accccagttg
      601 caaggattac agtagtagct tcatacatat catttccaga cattattgta aagtattctc
      661 ccatagcata aatattattt attcttttat tttcaattga aatatccata ctatctatat
      721 gtgatttaaa cttgtctttt aattcatcac cacttatatc ataaaaacct aaataattat
      781 ctattgaagg agctttaaca agcttattac ttaaattatc attaccaaac atgataattg
      841 ttttatttct tatttttgca tttatagcag cagacaatcc agctggacca ctgccgatta
      901 tagctatatc atatctcata atactcaccc attttttata aatatgattt tacttgcatt
      961 tcaattttgt tctttggcat gaatccaatt aaagtttcta ctggttttcc atctttaaat
     1021 ataatcatag ttggtactgt agatacattg aattgttgag ctatttctaa gctctcatct
     1081 atatcaactt ttacaaatct agctttatcc ttcatctctt cacctaattc tgcaaataca
     1141 ggtcccaaca tgttacaagg tccacaccaa gttgcaaaga aatcaactac aactactccc
     1201 ttactacctt caacactact tctaaattca gatgtattta taacttttgc catttttaaa
     1261 tcctcctatt aaactttatt atccctatat aattatacta tgaaatttat aaatataaat
     1321 ttacttttat aaggtctttt tatattttat actaattagt atacccattc ttcatttcta
     1381 aaaacaattt tttatatttt tattatttta acttttttgg tattgacaat tcaaatgtag
     1441 ttccaatatc tacatcagaa gttacattta tatcaataga taagtttaaa ctaatcatgc
     1501 gagctattga aagtcctatt ccagaacctt ctaaatcttt atttcttaca ttttcacttc
     1561 taaaaaatct atcaaatata agaggtatct cttctttttt tatgcctata ccagtgtcac
     1621 ttataagtaa agttacctca tcctctatat cttcttttaa ttctaatgct atctcatcac
     1681 ccaattttgt atatttaaag gcattatcaa tgaaaattaa tataagctgt cttaatttat
     1741 ttttatctgt tattataact ttattcttca gtttagaatt aaacacgaat cttttttcct
     1801 gaaactcagc aatgtcaata tagtcttctg atatttcttc caataatttt tctaagtcta
     1861 tttcttctaa attaacttta acaatagagt cctctttagt aagagacaat aaatcagtta
     1921 tcattttttt aagtcttcta gtttccttca ttgcagtagc tattgtttca acttcatcat
     1981 ttactgtact ttctggggat tttaacatac tttctaattt tgatgaaact attgttattg
     2041 gtgttcttaa ttcatgagaa gcatcttgta taaacttagc ttgatttttc catgctgttt
     2101 ctattggaat taaagctttt ctagtgagat atacagccac aaagtaagta attattacag
     2161 aaattaatat tccaattata agaacagatg taagctgtct taaagaattc atctcagaat
     2221 ctatatttct aattatctgt atttggtatc tacctacatc tacatttaat tctctaaaag
     2281 tatatccatt ttctgtgtat ttaaaaaacg aatttttttt atctagtctt ctatctggaa
     2341 gtaactcatc aaaatattca ttttgagtat agtaacttat ccttccatct tcatatacat
     2401 acaccatatc tttaggatct ttcagtctta ttgggttcaa aaatgaagtt cttttaaatt
     2461 gagagactat atattcaagt tcatcattta tctcactatc aattccacta tatgtaagtc
     2521 ccctaaaata tgagtatata aatatagaaa agattattaa aaaactacct actacaataa
     2581 tatacatttt tattaaatga tttttagtac tggtaaatac attcttattt atcatcgaat
     2641 atatatccta ccttacgttt agttttaata tatttatcat atccatattt acttagcttt
     2701 tttcttagat tacttacata tacttccact atttcaatag ttgcatctga atcaattccc
     2761 catattctat cataaatctg ctcttttaat aaaattgaac ctttatttaa tacaaaatac
     2821 tcaagcaagt taaactgttt gttttgaagt tttatttctt ctttttcaat atacactctt
     2881 ttttctagtg tatctaaata taaagattta aattctaatc cgtttctttc ttttatcttt
     2941 ccattagtcc ttaatatagc gtatacccta gctactaact cttccatata aaaaggtttt
     3001 gtcaaataat cattagctcc tatagtaaat gcttctactt tgtcatctaa tgtttctttt
     3061 gctgtcaata taagtacagg tgtatctata ttattaactc tcattttctt taggatatca
     3121 aatccattta tattaggaag cattaagtct aaaataacta aatcatatat attttgattt
     3181 actaagtata aagcttcttc tccatcttca cacttttcag tttcaaaatg cttgctaagt
     3241 tcttctacta cactttctaa aagtatctta ttatcttcaa ctattaaaac cttcataagt
     3301 atcccctacc tttatttgtc tatcttaact ttatgagatt ttaagtcaat atccacattg
     3361 gctttttctt ttatatcatc atacgtatat cccactattt caacactttt tgcctttgtt
     3421 gaacacaata tatctatatc tgtctcttct gaaggcttta aaatcttata tgatggtaca
     3481 cgatttactg ttaaatattc tccattttca ttaacttctg ctgtcttaag aataatattt
     3541 cctaaatctt ttgatgatga attttttact tttacttgat acgtaattcc ctcatttgat
     3601 tcatttactt ttttaagttc tgatgtagat agcacctcat actgactact attttctagc
     3661 ttaatattat tatttcttat attaatagta ttttctttta aattaacata cacaatctta
     3721 ccctctgcct catattcata tgcataaact tctacaccct ttacaaaagt tttatgccca
     3781 cattctatgt aagcaacttt cccaggcatt aaagtcatat ccaacaaagc cttactatcc
     3841 gaagatataa tttttttatt ctcatcatat tcattatata tcaattcaac attggatata
     3901 ttaaatgttg aattattttc tacctttgtt gatgtatcta ctttatcacc ttcatctata
     3961 tttataattt ctgattttcc tatattcaat acttttgcat ttccctcatc tatttttatc
     4021 tcatttttat cttctgtttg aatatcaact gaatcaggct gttcatcttt ttcacatgct
     4081 gtcaacataa aaataaatat tgttaatata atcattagag aaattgattt ttttactatt
     4141 ttattcatat taagcacctc atatagtagt attttatagt agatttttat actttttcat
     4201 aaattctttc aatgcctgtg taagtatttg atgtttttca aaattcgaat tactacataa
     4261 tctttcaaaa tcattccata tttctttatc tatacgtatt cttgttgttt ttaattcatc
     4321 tatgtgttca tcatctatat gtacatcttt tttattagat acttttttag gctttatatt
     4381 gtaatctttt acttctaaat accaatcata tacttcacaa agcttatcta aatcttcttg
     4441 agttatattt atttttctat ctttcttagg cttaacattt tgttttcgct ttgcattttt
     4501 taaacctgta tcattaaact caatttgttc aacactttta cttaaagcct tttcattaga
     4561 aatctttttt ttggtgctta ctttttttct attacttata gtattctttt tacttttttc
     4621 atactcctta gctttctctt cttccaagtt tctattatcc aaacttttat tataatttaa
     4681 ttcgtctttt aatatgtatt tacctttttc tgacttatat ccttttttat ttaaaaaagt
     4741 tcttaaagta ctttgactaa tctttagatt ttgagctatc tctgcaaatt taaatccttt
     4801 gctttgaagt tctaaaaatt tgtcaattct gtcttcattg ttattcatta atattcctcc
     4861 gtcaacatct tcttgtcttt cttttcatac ttcttattat attcttcttt tctaaatcta
     4921 ctctatttga ttcaattatt ttttgtagca ttttattata agtgaaatca tctaaattat
     4981 tattttttat atagagaaaa gtttcctctt cacatttaat aaaacaaatt gatagcaacc
     5041 atgccatacc cattttaaca taatattctt cactttgtat attattacag tattcaaata
     5101 tttctttaat atgtttatca tcaacataat atattaaaaa cataactaat gcaaatctaa
     5161 tttcccatgg attctttgaa taaacatatt ttttaagtat atcatacata ttttctttat
     5221 attttttcat agactttaaa ccagaacaaa acccatcgca aactgaccaa ttatcgattt
     5281 taggtataaa tctttttaca tagtataaag tttcttcaaa attactattt atattgccaa
     5341 ttaccatacc ttgtagccta acttcttcat agtatgtatc ctctgctact tctaaaaatt
     5401 ccttccaatt cccttggctt atttcttttg ataactttct taaattagga actctaatac
     5461 ctattatatt ttctgttcca ggtataagct ttttattaaa atctctatat tttatatctt
     5521 ttatagacat catatattct aaaagttcat tataatccaa tttattccat tttcccctat
     5581 caaacatcat ttaattgctc cttaattcct tttttcttat tgtaacataa aactctacaa
     5641 aaaaagcttc taactattag aagctttttc aaaaacataa gaaacttttc aattttaaat
     5701 ttaaactaaa ttaatgctaa tctcctacac caaagtctgt attattgtat aaaacttcta
     5761 tatttggatt ttcatccaag aatttatact tactaacaaa ccatttcact aactcttcat
     5821 ctctttttat actagaagaa ttatttataa aaacattcac tgtcgctttt tcaaaagtat
     5881 ttaggataat ctctatatct ttatctatca tctcttttgt ttgtcctttt attccaatca
     5941 taatacaagg tgaatcaaaa tattctttta catcttgtgg agtcttaaaa tttgcatttt
     6001 tatttaaaat attatttcta aaatcattgt caaaggtttc aactccaatt ttaaatacca
     6061 caggaatttc aaaatactct ctcatttcct taagcctatt tttgtaagac caatgacttt
     6121 ctaaaaataa tttttttata ttcttctctt ttataataca ctttattttt tctaaagtat
     6181 ccttaggtaa ttcaaaacag cttccagaat ttatgacttc aagaactcca tatttcccag
     6241 ttacattttt taaaaccctt agatttaatt tattcatctc ttcttctaaa ttagaattat
     6301 catctatata atcacaaaaa gaacactttc cccatataca aggaaatcct ttaagcagaa
     6361 caatttctct ttgattttta tttgttatct cactatatct atccattttc tatctatttt
     6421 cctccagtca atgatacctt taaagttttt ggaactgcat ttaatcctaa attcactaaa
     6481 acaactgcta acagcttatc tccataatca gtaaatactt gggttacaaa cacactaaaa
     6541 acatctccca gtcctaacac tttcaagatt tgcactatat aggatgaacc tgaagaggtc
     6601 atacctccaa ataaaaatgc tgaaattatt gaacttatta tagatgttgg aatagcaaat
     6661 aaaagtactc ctagtggagt ttttaaaccc cttaaaaaat tctttttaaa tgctattcca
     6721 gcaagtaatc cggttgatat ttgaactggt gcaaaataga gagaatacac atcaaacgta
     6781 attccactta ttaaacttcc aaacacacct gtaacaacag cgtatttagg tcctaaaagt
     6841 gaagctatca ttatagttcc tattgaatct aagtatacag gtaatcttaa tcccatagct
     6901 ataaaagcgc ctattatatt tatagaaata ccaaaagcta taagtgtaat agtttttaca
     6961 catatttttc ttttcacact atatcactcc ttccacagag tctataatgt cttcataacc
     7021 tttaaaaagc ctctttaaaa acatgtacat aaatttcttc tcatcaactt catttagaac
     7081 aattgcatta ggattttttt tatagaaatt gaaagaatcc actatagact gacccatagc
     7141 tattccatct tctacaactt ctacatatga ttcaaatcct ttacatatac ttctgtctat
     7201 aaagtacgct actgctagag ggtcatttat cacacagcca attattcctt cctgttccca
     7261 atggaaatca atataaaatc tagttatttc agttatatac tttgccatct tcttatcaag
     7321 tctatttata aactcaataa tattaggagt aagtacaatt tttctagtta catctaaacc
     7381 taccatgtgg atttttttag ataaattctt gtaaacataa tctgctccat gtgggtctac
     7441 ccaataatta aactctgcta ctggagagca atttccatga atcctaaatg ctcctcccat
     7501 agatacaaac tcatcgagat tttcaaatgc tttcttatct ttaataagtg ctttagctat
     7561 atttgtaagt ggtgcaagtg ctattattga tactttttca tgattatgta aagtatttat
     7621 tataaaatcc actgctccat tttttgcttt agctcctaca accttttgat aaaagttttc
     7681 tccaatacca tcttctccat gtgtatcttg agctgttaca agttttcttt ttagtggtgc
     7741 ttcttctcct atatatactg gaatatttag tgaagaacac atttgaagtg tttttagtgc
     7801 attttctgca cctatatttg ctggaacatt tccacaacat gtggtaattc caattacttc
     7861 tagctctggt gagtttaaag ccagaagaat tgccaaagaa tcatcaattc ctgggtcaca
     7921 atcaattatt acttttctct tttccatttt ttatcctcct tgatttgtgg tcgaggagaa
     7981 ctacacatct tttttccact ttcaaaaagg tgtgtctaag ggtattaatt atatactgcc
     8041 actatataat tccttagttt tttatacttg gagttctcga acaagtacag atgtaaaatc
     8101 atatttttag gctacttact atagactctt aaaaactata gactgttaaa aaatttttaa
     8161 acatctgatt taatagctat aaaataaatt ttatagcata aaagaattta ttatatttta
     8221 atgtatctta ttaaagttta tttagaatta tgaaccaact ttaataagat gtggcgcatt
     8281 taaaccgatt attattatac aataatatct taaatattac aattaataac attaatttat
     8341 tatccataaa aatatattaa cttatattat ttaataattt attgacagta ttaaaattaa
     8401 attatacaat aacagtgtaa attaacttaa caccgttaag caaagaggtg attatatgag
     8461 tatcaaagaa agacgtaaaa atgaaaaaga agaaatgaag aaaaagatta tggatgctag
     8521 catcgaaatt ataaaccaac atggatatga aaatttatca ataagaaaaa ttgctacaaa
     8581 aatagaatat tctccaacta caatttatct ttactacaag gacaaagcag agattatcag
     8641 cgatatgacc aataaacttt ataacacagt agaaagtaat gctattgaca ttatgaataa
     8701 ttgctcatca ctaccaatag ataaacaaat catagaaatt atgactagct ttataaaaac
     8761 tctttctagt gaaccagaga tggcaaaagc tatcatgcat agtagaatga atattatatt
     8821 tgcatctgaa aatacaaaca atactcctag caataatggt ataaaaatgc ttgataaatt
     8881 cctttccatt ggaattgaac aaggaatatt caaaaaaaat atagataaaa gctcatggat
     8941 gcttataagt gcattgttag gattcgtttt atgtgttgtt gaaaataaac tatattcttt
     9001 acataatttt tctcaactta tagaaaattt cttagatatt ttagtgggag gactttacaa
     9061 tgaaaattcg caacaaagct actaataaaa agaaaaaata tgtaaaaata tctattgctc
     9121 tattctttgt atttatatgc attattattg taattgtaag caacttaaag ccagaaaact
     9181 tggtagtaaa agatatagat attaataatg ttaaaaatgg catttatact ggaagtgctg
     9241 ataataactt ggtaaaagct actgtgtctg ttgaagttaa taatggtaaa atccaaaata
     9301 ttaatatctt aaaacacgac catcttttag gtaaacctgc tgaaaaaatt acaacaagta
     9361 ttattaaaca acagtcttta gatgtggatg ctataacttc tgcaacttat agcagtaata
     9421 ctatacgaaa agctgtagaa aatgctttaa gaaagggaga ataattatga atactattat
     9481 tatatattca agtaaatatg gttgtacaaa agactgtgca aatatactaa aaaataaact
     9541 atcagataat gtcactattg ttgatattaa taataataat aaaattgagt tatcaaaatt
     9601 tgataaaatt attattggaa gttctatata tgttggttca gtttcaaaaa aaatacaagt
     9661 gctatgtaat gataatgtag agttattaaa caaaaaacaa gttggcattt tcctttgctg
     9721 tggcttttct gaacaagcag ataaatattt aaaatcaaat tttccatcat ccttattaga
     9781 aagtgcaaat gctattggaa tttttggtag tgaagctcga ttagaaaaaa tgaaattttt
     9841 agataaactt attatgaagg ctgtttcaaa aggtaattat gacagtttta gaatatcaca
     9901 ggataatata gataattttt taataaatct taatagctaa aataaaacta attatttcta
     9961 tgataaattc ttttatttat aagtaatatt gctcaaaata gtctttaaac tttatcaaaa
    10021 ttttattttt cttagtgaaa tctaatgaaa ctagctctta atatatgctt tattaatcat
    10081 aacaaaaaga gagtgtctaa tgaactaaaa agttcattag acactctctt tttgtatgaa
    10141 atcaaatata tttttatcat ttcaaaagag aaaaatacac ttgtctctat gtttgacaca
    10201 ttctcttttt ataatataat atctaattta aaacctattt caaatattga catatactta
    10261 aaccatagac ttacaaaatc aattcatatt agcttaaaat ataatctttg ttactcaaat
    10321 tcctatacac aaccattatt tactttgata ctttaaaaca tcttccctat tttttattaa
    10381 attttataat aatagtccta ttaacgcaaa tactataaaa agagtttaac tttatccatt
    10441 aaataaattt aaattaaaac atatagaaat ggaaggagaa caatttatga taaaactaat
    10501 cggtatattg atagttgtaa ttggatttat acttaaaatt gacacattat ttactgtatt
    10561 attagcagga gtggcaacag gaattgttgc aggattagat tttaatcaaa tttttacaat
    10621 tttaggagat tcttttgtct caaacagagg tgtatcatta ttcatattaa cactgccagt
    10681 aatcggtgta ttagaaagat atggtttaaa acaaagagct gtttctttaa tagaaaaatt
    10741 aaaaagactt acaacaggaa aagtacttac tatatacatg atagctagac aaatagctgg
    10801 tgctctatca ataagaatga gtggacatcc tcaatttgta agaccacttg ttaatcctat
    10861 ggcacaagct gctggtttaa gtaatagtga tgaactaaaa gaatcggatg aagaagccat
    10921 aaaggcatta tctgctgcat cagaaaacta tggaaatttt tatggtcaaa acttatttgc
    10981 aggtagtagt ggagtattac ttatagcatc tactctaact caatttggat acaatgtaac
    11041 tggacttaat attgtaaagg ctagtactat tatggcagta atagcacttg tagtggctac
    11101 aatccagttt accttatatg ataaaaaatt aaacaaaagt cataaaaaat agttttattt
    11161 aggaggaata aaaatgcaaa attttataaa catatcattg gaaatctttt acgcactaat
    11221 gggattttta atgattgtta tagcatataa gtcttttact accataaata acaataagaa
    11281 atatggtaca tcattatttt ggatacttat atcactgcca ttcatatttg gtagattgat
    11341 tccagccaat ataataggaa taatattaat attatctagt ttattaacac tttctaaaca
    11401 agttgtattt gcaaagtatg aagaaccaga tgaaaatttt ggaaaagaac aagcagacaa
    11461 acttaaaaat aaaatattta taccatcatt aatattagca tttgctgcag tagtagtagc
    11521 tatgagcctt tctaattttg ataattcttc tcaatttgca ataggtgtgg gttcaataat
    11581 tgcattaata tcagccctta taataactaa agcaaaacct gctacatcag ttcaagatgg
    11641 ctcaagatta cttcaacaaa tgggacctgc tagtatgtta ccacaacttt tagttgcatt
    11701 aggagcttta tttactcagg ctggagtcgg cgaagttatt tcaaccatga tatctggtgt
    11761 agtccctgct gatagcagat tatttggtgt tatagcttat gtattaggta tggttatatt
    11821 taccatgata atgggaaatg catttgccgc attttcagtc ataacggcag gaattggtat
    11881 tccttttgta ttgtctcaag gtggaaatcc tgctataatt ggagcattag cattaacagc
    11941 aggatattgt ggaactctac ttactccaat ggctgctaat tttaatatag ttccagcagc
    12001 cttgctagaa tgtaaaaatg attatattgt tataaaatat caagctccag ttgctttggt
    12061 attaattata gcccacatat tagtaatgta ctttttagga ttttaatata attaggaggt
    12121 aaaataatga aaattttgtt aacaggattt gacccatttg ggggagaact aataaaccca
    12181 gctcaagaag cagttgaaag ggtcaataat aatataaacg gtgcagaaat cataaagata
    12241 actataccaa cagttatgac aaaatctgtt gaagctatag ataaagctat tcaagagcac
    12301 aatccagata tagtaatatc agttggacaa gctggaggta gatttgacat tacacctgag
    12361 agagttgcta taaatataga tgattttaga ataaaagata atgaaggtaa ccaggttata
    12421 gatactatta taaaagaaga tggtgaacct gcatattttt caaaattacc tgtaaaagcc
    12481 atggtaaaac atatgaatga aaataaaatt ccagctagtg tttcaaacac tgcaggaaca
    12541 ttcgtttgta atcatgtgat gtatggaatc ctttatatga tagacaagaa atatcctaat
    12601 ataaagggtg gatttataca catcccatat acaacttcac aggtaataaa taaaaaaaat
    12661 acaccattca tgtctttaga agaaatagta aaaggtttag aattagctat agaagcttgt
    12721 attatataca aagaagatgt aaaagaaatt ggtggtgaaa tttcataata aattttttag
    12781 tattttataa attaaaaata ctaactatta atttaaatat agactgtgag tagattattc
    12841 aaacaatttt actcacagtc tttttatggc atctacagat acaaaattaa ggtctattcc
    12901 tctctcccca ctcacacatt gcatctaaaa ttggaatcag agattttccc ctttctgata
    12961 aactatattc taccttagga ggtatttgag gatattcagt acgaataatc aagttatctg
    13021 cttctaattc ttttaatgat aaactcaaag ttttatagga aatagtattt atataacgtt
    13081 ttaatgcatt atatctgaca acttcaaatt ctacaagaca gtaaagaatt atcattttat
    13141 atttaccact aatcagtgaa agtgtataac taaatcctgt ttcacttaaa tctgtatttg
    13201 atatacagtc tatactcata tattacactc tccttttggt tagtattgaa caaaaatgtg
    13261 cgtacttgtt tttagaataa tttataataa aattatagtg taagaataat tgaaagtcaa
    13321 ttttataata ggaggtgtat ttactaaaaa aagagttatt ttaaatagga ttctaagatt
    13381 agacatttct atataaattg ataatattca tgaaaatcct attttataaa aaataatatc
    13441 tcttagaaaa tctattaaat aaaattcatt ggaggaaatt tataatgaaa gttttattga
    13501 taaacggtag cccaaaccaa tatggatgta cttatacagc actaaatgag attactaaag
    13561 tattgagtaa acacgatatt aaaacagaaa tactgtattt aggtaaagaa gcaatcccag
    13621 gatgtattag ttgcgcatcc tgttttgaaa ctggaaaatg tattcgaaat gataaagtta
    13681 atgaattgat tgaagatctc gataatattg atggtattat tattggttct cctgtttact
    13741 tttctagtgc aactggtcaa ttaacttctt ttcttgaccg tctatttttc atagctggaa
    13801 gtcgcatggc tacaaaatta ggtgcttcag ttgtgtcatg tagacgtggt ggtgcaagtg
    13861 caacatttga ccaactaaat aaatattttt caatcagcaa tatgcctatt gtatcatcac
    13921 agtattggaa tcaggtacat ggctttactc cagaggatgt tatgaaagat gaagaaggat
    13981 tacagactat gcgaactcta ggagaaaaca tggcatggct tcttaagtgt atatcagcag
    14041 gtaaaaaagc tggaattaaa gaaccacaat atgaagaaac aattatgact aattttataa
    14101 aataaataaa aaatgcacta atattaatta aaacaaaaca ctaatttata aataaaataa
    14161 gtataaatat aggataaaaa acactaaaac agaggtattg aattttatat caaacctcta
    14221 ttttattatt acattaataa ccaaaccata tataacatca tatatttcta aataaattat
    14281 aatattatat tctaatgtta ttgaaattac aataactgaa tttctgctca taaatttaaa
    14341 tattctacaa atctcatcta aataacaatt ttaatagacc taaaacaatc ctcatattgt
    14401 tcattttgta ttaaaaatat cacttacaat caaaaaccat ttattaagta tattcctaag
    14461 atatacaata tatatacatg gattggatag tataaataaa ataaatactt catactcttc
    14521 cctttttctc cattgtaaag taacattaaa ggtagagaag caatcatcat ccattggtaa
    14581 ttttgtaaaa tagaattttt taaactataa tctccatttg acattgttgt aaatataaat
    14641 atacacatca aaacataaca aatactcaaa ctgaactttt tacctctaca caaataaaaa
    14701 ataatcccaa gtataatcca aattggtcca ccttctacta aaaatggtac tggtaccaaa
    14761 atcataaata aagtaataac tctgttagtg actttaaaaa gtaaagctac tgtaaatatt
    14821 cctaaaatta taggaatgat tatcatcaat aatcctataa taaaatattt aatttctttc
    14881 tcttttctaa atttctttat gaactctatt gcttgtaaat aaatcgctat taaaaataat
    14941 gttgaaaaaa tgttatttat aattatagct ccttctggat gtgaaaatac attattaatt
    15001 atttgatttc ctatagccat aataacagac caaatataca atctacttat atattttttt
    15061 ctgttactag tgtgataaaa tccttcaaca gccatgaata taaacagtgg tgctgatatt
    15121 ctgcctaccc aatgaaacca aattggtatt tgcattgttt gtggcataaa tgctgctata
    15181 tggtcaaatg tcataaatat taatgctaaa atcttaatag taaacccact tatacctttt
    15241 ttaaaaaaat ccatatcata tctcctaatt ttgttttatc tctataataa tttttgttcc
    15301 tattccaatt tcactactta cataaatttt acctttatga atctctatta tttctttaga
    15361 aatagccata ccaagacctg aacctttatg catctcacct gtattagtac ctctataata
    15421 ttttttaaaa atatgtttca aatcactttt gcttattcca attccattat cttttattat
    15481 aatttctata ttatcttttt ttacaatttc tacacttatc aaagttcctt cgctattatg
    15541 aactattgaa ttaaatataa gattggttat agcccttctg aataagattg aatctacata
    15601 tacttcaatt aaatcttcat tactttcaaa ttcaatattc ctatttttat atctattatc
    15661 atttaaaata tcaattatta tatctctgac taaactgact aaatttattt ttttcttgtc
    15721 cagaactata gtattatttt ttaatcgagt agataaattt aaatcatcaa ctaaatcttt
    15781 tatatactta gacttattat atataatttc tgtatactct cgtatttcat cttcttcaaa
    15841 ctcataatct ttgtcattaa ttatctctgc atacccttgt atagatgcta gaggtgtctt
    15901 tatatcatgt gttatatttg ccagccattc ctctctcaac tcttcatttt cttttctctc
    15961 aacttcattt actcttaaag tatcagctaa catatttata tttttaaaaa cttcttcata
    16021 tatacctctg tccttaagat ataaactata atttccatta gccatagtct caatactcca
    16081 tagtacgctt gaaattggtt ttgtaagttt tctactaaat aaatatccta cacctaaagc
    16141 taccacgcta tcaattacta gtgttataat gataacttta tttaaagttt ttttaatgtt
    16201 attttgagaa taggttaaaa ttattcttga tatatctcta ctaggtatac ctactattat
    16261 gtttagatgt tttttgttta aatatttttc tagtacaaaa ttaacatact ttgtttctat
    16321 atacttgtaa ttattcacca tttgaaaggg tgtatatttt tttggagtat cctttggcac
    16381 atttactcca taaacttcct ctcctaaatc atttaaaacc tgtatccagg aatttgattt
    16441 ttctaatttt tcagctccaa tcttagatag tttaaattct ccatcttttt catataaatc
    16501 tttttcaaaa gaacgtgcaa attcttcagg attatttcca gaatctacaa ctttaaaaaa
    16561 gctattagtt gaaatcacat acaatataga tataatattt ataattacta ctgatatagc
    16621 tacaaacaca attgtaaata taaaatttct agtaatcttc cacttcatat tctaatcttc
    16681 ctttactgct aatttatatc caagaccttt tattgtcaga atatattcag gctttgaagg
    16741 attatcctct aatttttccc taatttttct tatatgaacc attatagtat tgtcaaatcc
    16801 aacataatct tctccccaca ctttatccca tagtttttct ttacttataa tttgattttg
    16861 attatgtgca aataaagata ggatttttag ttcctttgca gacagtccta tagattttcc
    16921 atcttttata agttctgccc tactttcatt taatatatat gggccaaatg atataatttt
    16981 ttcttcattc ttatttagat ttttactttc gttttgaaca tcactaaaat aacttaatct
    17041 tctcaaatgc gctttaactc taaaagccac ttccttaggg ctaaaaggtt ttgtaacata
    17101 atcatctcct ccaatcgcta gtccaagtaa cttatctaat tcttcggttt tagctgataa
    17161 aaatattatt ggagaattgg aggtttttct tatttcttta caaatatcat aaccttcgcc
    17221 atctggtaac ataatatcca atacaatcaa atcaggatta atacttttaa ataattctaa
    17281 tccttctttt ttagtttttg cagtatatat attattaagg ccctcttttt ttaggacagt
    17341 ttctaaaagc ttcaatattt ctacttcatc atctattatt aaaacacgat tttccataaa
    17401 caacctcctg taataattta catatatatt agcatatatt ttatctaaaa ttcatttatc
    17461 ttatttttaa ttaaactaaa atataaataa aattactatt atttttagtt aaaacctttt
    17521 gcttgatgta aaattttcat atgttatttt agttaacact atcattaata taattgagat
    17581 tactatagaa aaaactatca aaatacttgg tacattatac ctaaccattt gttggctcat
    17641 cataatccaa tttggagtca ctgacataaa ttttatagct ttatctggta taaaacctac
    17701 actaaagtat ggaattgcca ttatcaatcc acctagtata aatggaatta cagccgattt
    17761 aaatatagat gaaagtgtta agcctatttg tgcaaatata aaagaagcaa aaatagtagt
    17821 caatataaca attatataca tttgtattac tgtaagattt aaattactat ttccaaagta
    17881 ccataagtta tgaatcggtt catttaaatt tttaaaatca aaaaaactaa aacctctaat
    17941 tcctactaac aatataaaag aaatattaat aatagataaa attaatccat tagctactag
    18001 ttttgataat actactgatt tttttccctt tttagttgta tttattaaac ctgtcatatt
    18061 agaatcatat tctttaatgt aatttccaga agttattata aacattatca aacctattag
    18121 aggtattgag ccttctatct tgtaaaatat atctgcataa gtatctaaaa catcaaactt
    18181 tctaatcaga agaaatccca aaaatattaa aaacagccaa attatccaaa cactctttga
    18241 ttttaatatt tttatcattt cagctttaac taaatacatc atttctacta cacctccctt
    18301 gcatcttcca aatcaaaata aaacatgtat acatcttcga attttgcttg aactggttgt
    18361 atattatccc cacttggaat atcctctgaa acaattctaa gtactactga atcaatatca
    18421 ctttgaatac ttacaacctt atatttagat tgtatatcat caacttcaca ttcattttta
    18481 acttgaacat tatatacttt attttccatg tgacttaata tgtctttgtg tgttcctttc
    18541 attagaagtt tgccatcttt aatcattatt gtttcctttg caactgattc tatatcggaa
    18601 attatatgag tagaaagtat tattatctta tctctagaca tttttgctat taaattccta
    18661 aatctagctc tttcttgtgg gtcaatacct gctgttggtt catcaaagac tattatctta
    18721 gggtcgttta gtaaaacttg tgctatccca actctcctct tcatacctcc tgaaaatttt
    18781 cctatagact tatttctcac atcgtaaaga cctactaaat ctaataattc tcctatctta
    18841 ctatttaaat attttcccct taaacctttt aaagctccaa tatactgtaa aaattctttt
    18901 gctgtaaaat ttttataaac tccaaattct tgtggcaaat atcctaaaat atttctataa
    18961 ttagcatcca ttttaaatat atcctcacca ttataaagta tctgaccttc atcaggtttt
    19021 atcaacgttg ttatttgttt cattaatgta gattttcctg ctccatttgg tcctagaaga
    19081 ccataaactc cattttccag agttactgta atcttgtcgt ttgctacttt tttaccatat
    19141 atctttgaaa gattttctat ttctaattta ttcatcttaa ctcccctttt atctacttat
    19201 ataatctgat tttattacta aaaaacataa tgacatagca cctatcaata ttgccatcaa
    19261 aagtcctatt gctatattga caatgtctat attcacttta aatatcataa tatcacctag
    19321 ataatttcct attattttat taaaaccaaa tatcaaatca taataattta catttgatac
    19381 aactgaaagt aattttttag gtaatatatg gattaccgac attatcttag ttatagatat
    19441 aataaatata aaaaaactta ttgaagatat tgaagattta cttataaaag acccaaatat
    19501 ggctatcatg gcccatcctt caaacatcaa aagctctatg cctatattta atattacata
    19561 ctttgttata cttaaattag ttttcaatgc aactacattg tctactattc taaaagattc
    19621 taagtctcca ttaacaggta ctccttgttg catacaagtt ataataaata ttcccattaa
    19681 ataaacagaa taaattacta atggaactaa taaagcaaca aatattttag actttaatgc
    19741 ttttttcttc tcttttgatg atagtatgat atcttttacc gaagtaacga cttcatctac
    19801 gtaaatattg gatataatta tagatataat gattaacatt cctacattta taagtgaatt
    19861 tgtaactcta tagttaaata ctttccaaaa actaatatcc ttatattcat tcacacttag
    19921 attatcaagt tttttctttg acattaattc aattttttta gaaaattcac ctgtatctgt
    19981 ttgatttgaa aactgcttta aaacattaat cttattttga aatttctcat tacctatgtc
    20041 gataccactt ctcgtatctt ctatacatcc tttttcttta tctatataat aacgttctgt
    20101 ttctaaaaca ggcgtaataa agatactaat cattagtatt aacactaata tccctagaga
    20161 tataaacata ctcttagatt tagccaattt ttttatttcc caatttacca atatatttct
    20221 cctcctaaat cattttaatt acacatatat attaaaactt aaatcttata taaatataat
    20281 actaacctta aataaagctt aaattatttt ataatttata ggtgtgttta ttattggagt
    20341 tacatttatt atttttctgg gaaattatac attttcaatt atttttatta tttttgatat
    20401 ttttattgat tggaaatttt attgttgttc aaattgtagt tattctttaa atttaaagat
    20461 ggagtttttt aagaatagaa cctactgtcc ttattatgga aagattatta aatagttgca
    20521 tataacttta tttatcttga atcttatttt taaaaacaat tataatttta gaatattttc
    20581 tattctcaaa caagcatgtt gaataatgaa gcatcttcta gtattatata cttgtagaat
    20641 aaaactaaat tggtaaaact gggaatattt aaccttaagg agttaagtta tagaaactag
    20701 tactaaaaaa ttttataaat tatgttaatt gactaataga atgcgacttt ttatttttac
    20761 taaatttttt agggggatgg ataatatgtt tattggagaa atagacaaat gtacacatat
    20821 actaactgct tacatcagta gttcatatga ttattgtaat tttttagata cacagttaga
    20881 tgattttata tcagaatatg gagaaacggt agttgaaacc tgcttacatc aagtcttatt
    20941 attagtaagt agatacaatt aaaattaaat ttttctagaa aaaccttttt agatttatct
    21001 actctaataa atatataatt ttttgtactt tataagtaac tataacttct gctaaagtaa
    21061 agatgtattg ggagtacatc tttactttcc cattatagaa gttaaaagca tacaccataa
    21121 aatcaatcat ccaatacaaa ttatatattt cctatgtcta acttaattta caagaatcat
    21181 tctatatgca tattctgcaa tccactaaac atgctctaaa cctttactat tttatacatt
    21241 ctctataaat catacaaatc atatataaaa tccccctaat atgtttaaac cacctataaa
    21301 ataacaccaa tatttagaat attcaatatc aactgcataa ctggtcaata aaaacgtata
    21361 actagacaaa tttcaatatt tatataaata atacaagaaa aacaagcatt ttatttatat
    21421 aaatttttta ttt
//
LOCUS       DAMJVX010000054        21264 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMJVX010000054 DAMJVX010000000
VERSION     DAMJVX010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21264
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.056"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            68..141
                     /locus_tag="PC407_001794"
     tRNA            68..141
                     /locus_tag="PC407_001794"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100..102,aa:Gly,seq:tcc)
     gene            150..266
                     /gene="rrf"
                     /locus_tag="PC407_001795"
     rRNA            150..266
                     /gene="rrf"
                     /locus_tag="PC407_001795"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            540..2552
                     /locus_tag="PC407_001796"
     CDS             540..2552
                     /locus_tag="PC407_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2497243.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNGQDLEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TEDEIEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            2780..4693
                     /locus_tag="PC407_001797"
     CDS             2780..4693
                     /locus_tag="PC407_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497244.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTDNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEINTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEENVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLRMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            4686..6905
                     /locus_tag="PC407_001798"
     CDS             4686..6905
                     /locus_tag="PC407_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2497245.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEIALEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLISENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            7014..7844
                     /locus_tag="PC407_001799"
     CDS             7014..7844
                     /locus_tag="PC407_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDF2497246.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            7834..11301
                     /gene="addB"
                     /locus_tag="PC407_001800"
     CDS             7834..11301
                     /gene="addB"
                     /locus_tag="PC407_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDF2497247.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIEN
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDDYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGDNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSTICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            11301..15128
                     /gene="addA"
                     /locus_tag="PC407_001801"
     CDS             11301..15128
                     /gene="addA"
                     /locus_tag="PC407_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDF2497248.1"
                     /translation="MSSPKWTKEQLEVIGSRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIANISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDIDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNETESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDSPDSDY
                     YSEIEKKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            15174..16385
                     /locus_tag="PC407_001802"
     CDS             15174..16385
                     /locus_tag="PC407_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDF2497249.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVIATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            16372..19899
                     /locus_tag="PC407_001803"
     CDS             16372..19899
                     /locus_tag="PC407_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2497250.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNIQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFVVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKDLKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRS
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVINITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            20030..>21264
                     /locus_tag="PC407_001804"
     CDS             20030..>21264
                     /locus_tag="PC407_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2497251.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIK"
BASE COUNT         9174 a         2024 c         3772 g         6294 t
ORIGIN      
        1 gatatatatg tagtttttag ggtgctaact ctaaaaaatt agttttacta atttataaaa
       61 atttaatgcg ggtgtagctc aatggtagag ttctggcctt ccaagccagc tgtgagggtt
      121 cgatcccctt cacccgctcc aaaagattat gtggttacaa tagcagagag gatacacctg
      181 ttcccattcc gaacacagaa gttaagctct ctagtgctga tggtacttgg tgggaaactg
      241 cctgggagag taggacgtag ccacgtaatc tttttttgtt gtattaaatt taataaacct
      301 acaattgtta tatgttttta tttgtctttt atagattttt ttggcaataa atgatatagt
      361 gaagataatt aaatatattc aatatattta atttttttat tttttgtttt tataattttt
      421 ttagaagttt attttttatg taattattta taattttggg taaaaacact ttgatataat
      481 ttcaaaaatt aaaaggttaa aaaacaaagt agaaatttat gttaaataag gagattaata
      541 tgagaagaaa tacaaaatta ttaacaacag ggattctttc aatggcaatc gtcgcaccta
      601 caatggcatt tgctactgaa tctaatgcta tggaaaataa cgctgattta aatataaact
      661 tagagaaaaa aagtatcgtt ttaggtagca aatcaaaagt tagtgtcaaa tttaaagaaa
      721 aaccagatgc agatagcatt acattaaagt ataaatgcta tgacatgcca ttggatacaa
      781 ctctaaatta caatcaatca actggggcat atgaaggaac tatcaattat aaccaagacc
      841 cagaatatct aaatgtttgg gaactacaag ggataacaat aaacagcaaa aataatccta
      901 aaactttaaa cggacaagac ctagaaaaga tgggattaaa tttaaaagac tataatgtaa
      961 cacaggaatg tataattgaa gacataactt ctagaaaaga tgtaaataaa tatttgagaa
     1021 aaacttctgc acctattaca gaacttacag gaagtgatag atatgaaaca gcagttaaaa
     1081 taagtaaaga gggctggaaa aatggttcag ataaagtagt tataataaat ggagatgtaa
     1141 gtatagatgg cattatatca actccgctgg caaccacata taatgctcca atacttttgg
     1201 ttgaaaaaaa caatgtgcct aatagtgtaa aatcagaatt aaagcgccta aaccctaaag
     1261 atataattat aattggagat gagaatgcta tttctaaaac tactgctaat caaattaaat
     1321 cgactgtaaa tgctagtcaa acacgtttaa atggttctaa tagatatgag acatctttat
     1381 tgatagcaaa ggaaatagat aaaaatcatg atgtggaaaa agtatacata acaaatgcta
     1441 atggcggaga agtggatgca cttactatag cagcaaaagc aggtcaagac aagcaaccaa
     1501 ttatattaac tgataaagat agtattacag acaatacata taaatggtta aagagtgagg
     1561 atttacaaaa tgcttatttt ataggtggtc ctcaaatgat atcaacaaat gttataaata
     1621 aggtaaatgg aataactaag gatagtgtta ctaataatag agtatacgga gcagatagac
     1681 acgaaacaaa tgcaaatgta ataaaaaaat tctatacaga ggatgagata gaggctgttt
     1741 tagtagctaa atcagatgta cttgttgatg ctttagcagc aggtccattg gctgcgaact
     1801 taaaatctcc aatacttata acaccaaaga cgtatgtatc tgcataccat aaagataatt
     1861 tagaagctaa atcagctaat aaagtataca aaataggtgg aggattgact tctaaggtaa
     1921 tgagctctat agcatcatca ttatctaaac acaatacgac tccaacagaa ccaggaaata
     1981 gtgggggcaa gacagttatg attgacccag ggcatggtgg ttcagcacct ggaaattcat
     2041 ctggaggaat gattgaaaaa gattacaatt taaatacttc acttgcaaca actgaatatt
     2101 tacgttcaaa gggattcaat gtaataatga caagagacac agataagact ttatctcttg
     2161 gaaatagaac tgctctatct aattcattga aaccagattt atttacaagt atacattata
     2221 atggctcaac taataaacaa ggtcatggtg tagaagtatt ttataagctt aaagataaaa
     2281 atggggggac tactaaaact gtagctacca atatattaaa tagaatttta gagaaattta
     2341 aacttacaaa tagaggtata aaaacaagag tacttcctag tgattctaca aaagattatt
     2401 tatacgtttt aagaagtaat gatatgccag ctgtacttgt agaatgtgca tttttggata
     2461 atgaaaatga tatgagttta ataaactcat ctgcaaaagt aaaagaaatg ggtacacaaa
     2521 taggtaaagg aatagaagat tcattaaaat aaatggaaat aaaaatattt aaaaacttaa
     2581 tgtaaattta tactaaagtc aaaaaatgaa atataagtgt atcaatagta agactgtatg
     2641 tacatttgca catgtgaatg caagtatata tacagtcttt ttttgtagtt aaatgcaata
     2701 atttgattgt gttttgtaaa atacacaata tgatataatt agataatata cacttaattt
     2761 aattgaggaa ggtgattata tggaatattt atttgaaaaa agtagtttaa aagaatatgg
     2821 aaatgaagct ttagataaat atagagaatt attagataaa actgataata attcaaggtg
     2881 tattctttta ttagtaccaa acaataacac aaagatacga tatgaaaggg ctctaagatt
     2941 aaattatagt gaagaactga aaataacaac atatataagt tttgtgaaga aagaattagt
     3001 taaatactgg ccactaataa tagaaaaatg tgatgaaata aatacaaagg ttgtatctcc
     3061 aacatttata tcaaatagtt taagtgaata tttaatagtg caaaaagtta aggaaaaaag
     3121 gatacaagaa ggatattttg aagatattac aggaaccaat aaatccatag caaatagtat
     3181 aatgactaac ataaataaat ctgcatttaa tttaattgat tttgatttta ttggagaaaa
     3241 aatatattca tcaaagaaaa ataaagatag tatatataga ttttcataca ctcagatgga
     3301 tgaaattata tcatattata taaattcgtt actatcaaat tcaattttag ataatgcatt
     3361 aagcatatat ttatacaatc aatacctact taatgatgat ttttatttaa aaaacttatt
     3421 tacagaagtg agatatataa ttgtggatag tcttgaaagc tcaagtaatg cagaagttga
     3481 ttttataaca gaagcattgg ataatacatt acaaagttat atatattttg attatagtag
     3541 ggattattct gtatttaata atgtggatat agactatata aatgagaaaa taatatcaaa
     3601 aattaagcct aaagaagaaa atattaaaaa gaaggagatt agaatagaag atttatactt
     3661 attacctgca aatatagaat taaatcaatc aagtcaatta tacaatgaaa tgttagattt
     3721 aataagtgaa aaagttatat ctcttataga agaaaatgta tctccaagag atatagcaat
     3781 tatatctcct ataaacaata gtatattgga gcatcaaatt agagattctt tgatagaaaa
     3841 aaatatagat gtttttagta ctaagaagga taaaaaagct gtagattatc catatgggaa
     3901 tgcattggta gtggctacat gtatatttta tggatattta gattttataa aagatgatga
     3961 atacttaagc tttttagaaa ctcttttaga aataaatagg ataaaagcat ttaagatttt
     4021 taatgaaaca agacatttaa gtattgataa tatagatgaa catagtcagt atcgtgatat
     4081 tttacagtat attgaagaaa aaaagaagag tgatataaaa atacatgaat ttttaactca
     4141 attttacata gataaaatgt taaatttaaa agaaggtaaa aagaatgttg ggttatgtaa
     4201 aaagataata gctgaaagtg agtcttttag tgaaagttta agtctactta ggatgaaaaa
     4261 agaaaagata tttatagaag ctttaaaaag tacaataaac gattattact ctgttgtcga
     4321 tatagaagag ttgaaatcca aagacaaaat agtaataact actccttatt catatatatc
     4381 atcaaatata gatagaagta tacaaatatg ggtagatata ggaagtaatg cttggaatat
     4441 gaaaatagaa aaagatataa gtaatttaat tgtacttaga aaatcttttg aagaaaaaaa
     4501 gatttataca aatgaaatgg aagaatatta taaaaaatat tatctataca atacagtata
     4561 taatttgtta ttaaatgcaa agaaaatcta tgcatttaaa agtgaatatg cgattaatgg
     4621 ttatatccaa gagagtatat tatatagtat acttttaaaa atctcacata aaggagataa
     4681 aaattatgat taatataaat tatagagaag accagatacc aataataaat tacgataatg
     4741 gtactatggc agtaccagca gtaccaggtg ctggaaaaac atttataata acaaatttag
     4801 tagctaaact attacttgaa cagaaacata agggtggtaa aatattaatt cttacatata
     4861 tgaatagtgc tgtaaataat tttaaaggta gaataagaaa gattcttgaa gagaataaaa
     4921 tagatgatac taattcgtat gaagtaatga ctatacacag tttggctgta aaaataataa
     4981 aggaaaagcc agaagttgta atgttaagtg aggattttaa catagctgac gatttgcaaa
     5041 aaagtattat attaaatgaa tgtataagta gatttagatt tgaaggtgga gaaagagctt
     5101 ttagatggtt tttaaaggag caaaaagatg aacgctggaa agaaatagct cttgaagcct
     5161 gggaaagagg tttttttgaa tttgcaggaa atgcaataag tgaacttaag tataagggaa
     5221 tttctccaga taaacttgaa gaaatattgt caagaggaca caaaggaatg cttaaaatta
     5281 ttttaccaat atataaattg tatgatagaa aattaaagca aaatggattg ctagattatg
     5341 atgatatatt aattttagca gaaaaaactt taagcttaga cgaagggctt agaaagaagt
     5401 ttcaaactag atacaaatat atatttgaag atgagtgtca ggactcaaat gaaattcaag
     5461 gtaatataat aaaaattata tctagtgaaa ataaaaacct agttagagtt ggagatataa
     5521 atcagagtat aacaggaaca ttttcatcat cagaccctaa attttttaaa gaatttataa
     5581 atagtgcaga tttctgttat aggatggata tgtcaaatcg aagttcaaaa gatatacttg
     5641 atttagcaaa cactcttgtc aagtatgtta caaaggaatt tagacaagaa gaatgtagac
     5701 atgctcttga agatatgcaa ataaaaactg tgccaaatgg aatgggatat aaagaaaatc
     5761 ctaatccaga acattataat ataaatgtaa agtggtatga gagttggaaa attgaaatag
     5821 agcaaactgc tagatacgtt aagggtataa aaaataaata tccagataaa agtataggta
     5881 tattggttcc atttaatgaa caagtgacac aagttgctaa agaacttaga gagaataatc
     5941 taacctttga agaattagga ccaaattctt tgaataaaag gaaggttata aacaacatag
     6001 catatattat tgattttatg ttacattgtg atgatgtaga aaagttaata gttgtactta
     6061 ataaagtttt tatagatact gataatgaag aagaaataaa gtatttttta gaagaattaa
     6121 aaaactattc taccgaagaa attatttata attttgaatt tagtaagtta gataatgatt
     6181 taatagaatt taaagaagaa agtgacatat atttaagttt taaaagagga atagatatta
     6241 taaaacagat attagaatat ccaataataa ggttagacct cttagttttg tttattggga
     6301 aaaaacttaa tttagaaaaa gaagataaag ctgtactgga ttatatatct ttttatataa
     6361 agtatctaat atcagaaaat ataaatatgg atttagaaga tgtatataat ttattgtttg
     6421 atgtaaagaa tagggtgttt aatcatataa tagatgtggt atatgagata aatggatatg
     6481 aaccagagcc cggtagtata acactatgta actatcataa gtcaaagggg atggaatggg
     6541 attgtgtgtt tttactaggt cttgttgaat ataatttccc tgacaatatt aatcaaaagt
     6601 ttcagagtga taaatggtat ctaaaagaaa aatataaaaa ccctatggca attattaaat
     6661 cagaagttga agctatatta aaaggaagta tttcaacaga ttatgctcat aaaacgaaga
     6721 ttgattctat aaatgaaaag attagacttt tatatgtagg gattactaga gcaaaggaaa
     6781 tgttagtact ttcaggaagt gcttatagag atgaaagtga tatagggaat aagagaaaag
     6841 agcaaaaacc atgtatatat ttaagcagac ttaatcaaca tattattgaa aaaaggtcaa
     6901 attaatatat aataagttat taaaagattg cttgaaatat atctaaaagg ggataaaaca
     6961 atatgtacag taagtaacta ctaaaaaata aagttaagag agaagtgata tgagtgaacg
     7021 ataaattaaa atatttttct tatagtcaaa attcaatcaa cacatataaa tcatgtccat
     7081 tgaaatttaa atataaatat attgatagaa taaattggaa aaatgatgat gttggtagta
     7141 gagagtatta tgaaacttta aaaacaggaa gagattttca tttgatttgt gaaagatatt
     7201 ttagcaatat acctcttgga atctatttta atgaggatga taaaaatagt aaaaagtttt
     7261 taaagtggat ggaaaatata aaaaaagttg ttcctattga aaaaggaaaa acgtatttgc
     7321 cagaatatga agtaaggatg accttaaatg gggatataat acaggcaaag tatgatttag
     7381 ttgtaattgg aaataacaat atagaaatat gggattggaa aaccgagtct aaaaagattg
     7441 actataaaca cgtagaaaat agaatacaaa ctattgttta tatgttttta gcaaaagaag
     7501 taatacctaa agttttaaaa atggacataa atgttaaaga tataaatatg agatattatc
     7561 aacctgaatt tgatgactta ccaattacca ttttatatga tgaagaaaaa catgaagcaa
     7621 atagaaataa aattcaaaat tatataagta tgataaaaaa cactaattat gaagaacata
     7681 catacgaaga caatctatac aatgatatag aaaaggtata taataaagaa gaacgtatgt
     7741 gctatagaaa taaaaagcat tgcaagtatt gtgaatttaa taagttatgt aatggcaaag
     7801 aaatagatta tagtattttg gaggcggaaa tttatgggac ttaggtttgt attaggtaga
     7861 agtggaagtg gaaaatccac ctatatacta gatgagatta aaaaagaagc tcaaaaaaat
     7921 gaaacgacat caattatact tttagttcca gaacagtaca catttgaggc tgaaaataga
     7981 gtaagtaagc tattcttagg aaaagaaaaa gataagtatc taagagttag ggtacttagc
     8041 tttaagacac taagtaatat agttttttct caagtaggag gattgacaga tgtaaatata
     8101 aattctagtg gaaaagcaat gatggtatat agagctattg aagatgtaag tgaagaatta
     8161 aatgtgtttt caaaatcaaa atcgcagtct ggttttgtaa gctcaattac agatatgata
     8221 tcagaaatga aacaatacaa tataagccct gaaatgttag aaaatatatc tggagaattg
     8281 gataatgaaa ctttaagttt aaaattaaaa gatataagta aaatatataa ttcttttgaa
     8341 gggaaattgc atgaaaatta tgtagatgct caagatatgt taacttcatt ggcaagtaaa
     8401 attgaattaa gcagttattt agatggagct tgtgtttata tagatgaatt tacaggtttt
     8461 acaccaaatc agtacaatgt cataaagagt atattaaata agtctaaaag tgttaatatc
     8521 tctttgactg tagatgatat caactatatt gggtacagta aatctgatat gttttctaga
     8581 acgaaattta cttattcaaa attgactcaa ttgtgcaacg aagaaggcat aaaaatattg
     8641 ccacaagtaa atttaaacac aggtgttata aaaagatttg aaaaagttaa ggaattacag
     8701 cacctagaaa gattttataa tgcttatcca tataaaatat attcaaatcc aactgaaaat
     8761 ataaagataa aagagtttaa caatttatat tcagaggtgg aagagatagc cagagaaatt
     8821 gtacatttag ttagagataa gaatgtaaga tatagagata taaccattgc tacaagagat
     8881 ttaaatagat atgatttttt agtacactct atatttaatg aatataatat accaaacttt
     8941 atagataaga agagagaggc aaaaagtaat ccaatagtta ttttaattat ttcagcttta
     9001 gaaatgaaga atagacgata tgggtatgaa actatgttta ggtatttaaa aagtggactt
     9061 ataggaattg ataatgatga tataaattta ctagaaaatt atgtgttggc taatgggatt
     9121 aaaggtaaaa aatggtttga tgaaaaatgg gattatagaa taacacaaag tctttcaggt
     9181 caagaaagtg aatttgaact ggaattaaaa gaaaaaataa atgaaataaa aaatagagta
     9241 ttagagccta tagtaatttt acaagaaaaa ttaagaggaa aaaatagagt aaaagaaatc
     9301 tgtagatata tatatgaatt tttattagat ataaatatgc cagaaactat agaaaattta
     9361 atagtaaatt ttaaggataa aggggaactt gatgttgcaa atcaatactc acaagtttgg
     9421 gacatagtag tggacatatt agaccaaatg gtggaactta tgggtgatga aataatttct
     9481 ttagaaaaat ttataaaatt aataacctta ggctttgatg aatatgaatt aggactggtt
     9541 cctcctagta tagaccaggt acttgtaagt agtgtggata gaatgaaaaa tccagacacg
     9601 aaatatttat acctagttgg aactactgat ggtgtgtttc cactgattac aaaggatagt
     9661 ggaatattga gtgataatga tagggaaagt cttggaaaca aaggaatcga agttgatatt
     9721 gatagtaaga ctaggagttt tgaagaacag tttcttgtat ataaagctct tacttcgaca
     9781 tctaagaatt taaccataac atatccaata tcagaccatg aaggtaaaac gcttagacca
     9841 tcaataatta tatcaagact taagaagata tttccaaata tagaaaataa aagttattta
     9901 gtggaagaaa ataaaagcac tgataaagat atacttaaaa aaataacggt taaatcccct
     9961 acatttaatg aacttataaa cgtaataaaa aattatgaca gtgatgatta caatacagaa
    10021 gagattaata gtatttggtt agatatatat aggtactatt taaaagatga aatctatagt
    10081 tctataacaa aaaaagttat aaaaggtctt agttatacta atcaagttca taagattgaa
    10141 gagaaaaaaa taaggtcatt gtataaaagt aattctctta gcatatctag gcttgaaaaa
    10201 tatgctgaat gtccatttgc ttattttata caatatggtc taaaagcaaa aaagcgtaaa
    10261 gagtattcat ttacaccacc agatttagga acttttattc ataatatttt agataggttt
    10321 tcaaaagaac ttttacaaga taatttaact tggagagata ttgatgaaaa atatatagaa
    10381 ttaaagatag gtataatagt tgatgaaata attttaaaga taccaggata tatattaaat
    10441 agttctgaga ggtataaata ccttgcatat agattaaaga atatgttgac aactgctata
    10501 actataatta gtcagcaaat aaaacaaggt tcatttgaac caatagatta tgaagttaaa
    10561 tttggcgaca atggaaaata tccacctata aaaatggtgt tagaaaatgg acaagaagta
    10621 agtttaattg gacaaataga tagagttgat gaatttgaag aaggagaaaa taaatatata
    10681 agaataatag attataagtc aggcaataag tccataagtc taacagaaat atattatggt
    10741 ctacaattac aattacttgt gtatttagat gcaatccttg aaagtgcaaa ggatgaagat
    10801 ataaatataa atccagctgc aatactatat tgtagaataa ataatccaat agcaaaattt
    10861 aatgaagata aagatgatga agaaatacaa gaagcaatat taaaagaact tagaatgaag
    10921 gggcttgtag taaaagactc tcacatagtt aaagagatgg ataaatcatt aatagatgga
    10981 gaaaggaaaa actctttagt aataccagta ggtcttacta aggatggaaa tgtaggtaaa
    11041 agtacttcag caataagcta tgaggatttc aaactgctta gaaaatatgt aaggcatgct
    11101 ataaaggatt tatgtgaaga aatgttaagt ggagaaatac gtatagctcc atataaacat
    11161 aaagatggaa catcatgtga cttttgtgat tactctacta tatgtcagtt tgattcaact
    11221 ataaaagata acaagtataa aaatttaaat aataagagta atgaagagat aataaaaatg
    11281 atgaaaggag atgttaatta gtgagttctc ctaaatggac aaaagagcaa ttagaagtta
    11341 taggaagtag agaatgcaat ttattggttg ctgctgctgc tggttcagga aaaacagccg
    11401 ttctagttga gcgtataatt caaatgataa caagtagaga aaatccaatt gatatagata
    11461 agttattagt tgttacattt actaatgctg ctgcgtcaga aatgagagag aggatagggg
    11521 atgctatagg aaaagcctta gatgaaaacc cagaaaataa acatttacaa aaccaacttg
    11581 ttttattaaa taaatctagt attacaacaa tacactcatt ctgtttggat gttataaaat
    11641 caaactttca tagaataaac ttagacccta atttcagaat aggagaccaa actgaatgtg
    11701 ctattttgaa acaagaagca atagaggaag tatttgaaga tttatatgaa gaaagagatg
    11761 aggggttttt aaatttagtt gaaagttatg cagaaagagg aggagataaa gaagtacaag
    11821 atattatctt gggaatatat tcatttgcaa tggcttctcc tgaacctaaa aaatggttga
    11881 ttgactcagc agaacgattt aatattgatg aaaattttga tttttcacag tctatttggg
    11941 ctagagctat attagacact gtaaaaatag aaattaatgg tctttgtcta aatatggaaa
    12001 gggcattaaa agaagtggaa tcaatagaag aattagaaac ttttgcagaa aagttatccg
    12061 ttgaatataa aaaaattgct aacatttcac aagcatgtaa taaatcttgg gatgaggcat
    12121 ataaaaagat ggcaagtatg tcctttgaaa attatgtaaa aggagtaaaa agaatttcaa
    12181 aagatgctcc ttcgtatata aaagaatcaa aagaaaaggc aaaaactata agagataaga
    12241 ctaagaagtc tttagaaagt atagtaagtg ctacattcaa taaagataat gattctatta
    12301 gagaagaaat aaaatatctt tataacatag ttaaaccaat atctagtgtt gtactgagat
    12361 ttgaagagga atatagcaat aagaaaagag aaaaaggaat aatagatttt aacgatatag
    12421 agcattttgc attaaatata cttacagata tagatgaaaa aggtaatatt gtaccttctg
    12481 atatagccgt tggatataga aacaagtttt atgaaatatt tatagatgaa tatcaggata
    12541 gtaatctagt gcaggaagta ctgcttaaag ctgttgcaaa tacagaaact ccaaatagat
    12601 ttatggttgg tgatgtaaaa cagagtatat atagatttag acaagcaaag ccagaattat
    12661 ttttgcaaaa atataataac tataatgata aaaagggaag ttcccataga aaaataatgc
    12721 tttacaaaaa ttttagaagt agagaagaag ttgtagatgc tgtaaattat atatttgaaa
    12781 atataatgaa tgagaatata ggagaaattg aatatacaga aaaagaaaga ctaaatttag
    12841 gtgcgaattt taatgtggat acagacgaga aatctattat tggtggagca acggagatac
    12901 acctaataca aaaagataat aaacttgatg atgatattat aaatgataaa gacgatagaa
    12961 ttaataacaa agaaaatgaa atagaggaag aagaaaaatt agataatata caacttgagg
    13021 ctagaatggt tggaaacatt atcaaagatt taatgaaagt caatgaagat ggaaaaattc
    13081 aaaaggtcta tgataaggga atagatggat atagacctgt ggaatttaga gatatagtta
    13141 ttcttttaag agctacatct gcatgggctc cagtgtttgc agatgaattg atgaatatgg
    13201 atataccaac atatgctgat gttggagtgg ggtattttga tacgattgaa ataaaaacaa
    13261 tattgtcatt acttcaaata atagataatc caatgcaaga tatacctctt atatctgtat
    13321 taaaatcacc tatatttgga tttacaccag aggatttgat tgatattaga gttcagagta
    13381 aagataaaat tttttatgag gttttaaaaa gtacagcaga gtatgatgga tttacagatt
    13441 ctcaaaatga aactgagtct gagtttatac caagtgaaga gtgcataaac aaaagtaaag
    13501 attttttaat taagctaaag gaatttaaag aaaagtcaat gtacatgagt acagatgaat
    13561 ttatatggta cttgtacaca aggactggat actatgctta tgttggagca ttgccaggtg
    13621 gaagtcaaag acaagctaat ttaaaagtat tgtttgagag agcaaaacaa tttgaagaaa
    13681 ccagtcttaa aggaatattc aactttgtaa attttataga gaaattaaaa aaatctagtt
    13741 ctgatatggg aagtgcaaaa acacttggtg agaatgcaaa tgttgttaga ataatgagta
    13801 ttcataaaag taaaggtcta gaatttccag tagtcatatg ctctgcaatg ggtaaaaatt
    13861 tcaatactca agattttaaa aagagtattt tatatcatca taatttaggg tatggacctc
    13921 aatttgtaga ctatgagaga aggatttctt ttccaagtat agccaaagaa gcattaaaaa
    13981 gcaaaataaa tatagaaaat ttatcagaag agatgagagt tttatatgtt gcatttacaa
    14041 gagctaaaga aaaattgatt ataactggtt ctactagaaa tatacaagat agtataaaga
    14101 gatggtcaaa tggaattgaa agtttagata caatatcaca atacgagata ttaaaaggta
    14161 aaaatttttt agattggata atgccttgtg tattaagaca tagggattta tctaatctac
    14221 tagaagaagt aggattagac gcagttttta atgttgaaca taattcaaaa tggtatggaa
    14281 agttgtggaa taagaatgat attttagtgg aaaagaagag tgatgaagaa aaagaaagta
    14341 ttgaagaaat tcttgaaaaa atagatgtag atagtccgga cagtgattat tacagtgaaa
    14401 tagaaaaaaa attaaactat atatatccat atgaatttag tacaagaaaa cctgctacta
    14461 tatctgttac agaaataaaa aagatacaaa ataattatga agaagagtta ataaatacta
    14521 tatttgaaca aaaagtaata ttaaaaaagc ctctatttat tcaaaatgaa gaagaaagag
    14581 aaaaaataag tggaacagaa agaggtacaa tagtccattt ggttatggag gttttagact
    14641 taaaaaatgt aagtagtgta aatgatataa aatcacaaat aagaggtttt gtttcaaaag
    14701 gtataataac agaaaaacaa gctagtatag taaatccata taaaatatat aaattttttg
    14761 cctctaacat aggaaaaaga atgttgaatg ctgaaattat aaatagagaa aaatcaattt
    14821 atgcacaagt aaatatgaaa gacatatata tttatgaaaa gcttataaac aatgatgata
    14881 aaaaacttta tgataatgaa agtgtcatgt taagaggtat agttgatgct tattttgaag
    14941 aagataatca aatagttttg gtggattata aaacagattt tgtaaatgag gaaaatataa
    15001 atcaaataat agagaaatat aaaaaacagt tggatttata tgcagatatt attgaaactt
    15061 taactggtaa gtcagtaaaa gagaaatgta tatatttatt tggagttgat gaagctgttt
    15121 gttattagtt tttatatata aattaaacct aaaagataga ggaaggggaa aaaatgagat
    15181 ttattcatac atcagattgg catctaggga agtccctaga aggccattct agaattgagg
    15241 aacaagcaaa gttttgtgag gaatttataa aaatagtaga atcaaatgaa atagatatgg
    15301 ttataattgc tggagatgta tatgatactt ctaatccccc agcacaagca gaaaaactat
    15361 tttatcagac tgtatcaaga cttgccaata atggtcagag gtgtgtatta ataatttcag
    15421 gaaatcatga taatccagag agactttctg caataacacc tctagcacat gaacagggga
    15481 tacttatata tggttatcca ttaagtgcta cgatagaagc taagtataaa gggtttgaaa
    15541 taacatatgc aactcaggga tgtacaaaac tgaatataaa tggagaaaat attgtgattg
    15601 ctacattacc ttatccaagt gaaaaaagat taaatgaagt tttttcaagt gaagatgagt
    15661 ttgagaaaca aaaaaattat tctgaaaagg taggagatat atttagaagt ttagaagaaa
    15721 attttagaag tgatactata aatatagccg tatcacatat atttgtaata ggtggagaga
    15781 gtactgaatc agaaagacct atacaattag gtggaagttt tttagttgaa agaaaagatt
    15841 tgccagaaaa ggctcaatat actgcactag gtcatttaca taaacaacaa aaagcttccg
    15901 aacgtttaaa tgcatactat tctggttctc cacttcaata tagcaaggat gaaagagcgt
    15961 ataccaaagg ggcatacata gtggatataa aagctggaga aaaaccaata attgaagatg
    16021 tatattttaa taattacaaa cctatagaag tatttaaatg taatggaata gaagaagcac
    16081 tagatatatg tgagcaaaat caagatagag agatttggtc atattttgaa ataaatactg
    16141 atgagataat atcacagaat gaaattaaaa aaatgaagga actattaaaa gacataatag
    16201 aaataaaacc aataataaca tcttgttatg aacaagaaag tgtagatata aaagaaaagt
    16261 ctatggcaga gttgtttaga gagttctatt cttttagtaa aggtgtagag ccaaaaggag
    16321 aacttatgga tttatttcta gatataataa gtgaagaagg tgaatcagca gatgagacca
    16381 attagattag aacttacagg acttaatagc tatattgata aacaagttat agactttgaa
    16441 aaattaattg aaagaggttt gtttgggata tttggaacta ctggaagtgg aaaatctaca
    16501 atcttagatg caataactat tgctatgtat ggaaatatat ccagaaatac aaaagaatat
    16561 ataaacagtg tgtgcgataa ggcaattata tcatacgagt ttgagatagg aagcaaaaac
    16621 actagaagaa gatacattgt agatagaacc attgctagaa gcaaaacagg aacaaagacc
    16681 tctaatgcta gattaataga agtattgaat gacaatatcc aaaatgtttt agctgataaa
    16741 gttgtggaag taaatgaaaa agtagcacag gttgtaggat taacggcaaa tgattttact
    16801 aggtcagtag ttttaccaca agacaaattt aatgagtttc taagattatc acgtgcagat
    16861 agaagagaca tgttagagag aatttttaat cttgagaaat atggaagaag tcttggagaa
    16921 aaagtgaaaa agagaaaaaa tatacagtta caaaatttaa aagatttgaa atcaaaatta
    16981 agtcaatatg atggaattac agaggaagta tacaacaata taaaccaaga attaatagag
    17041 ttaaaaaatt tggaaaaaga taagaataat gctttagacc tagctcaaaa atcttatgaa
    17101 gaaagtaaaa cagtatatga agaacaatta aagttagaaa aaaatgaact tagaaaaaat
    17161 gagcttgatt taaaaaatag tgagataaaa gaaaagattg ctcttgtaga aaatgatagt
    17221 aacgctaaaa aggtagaccc atacataagt tcagttcaaa atctagaaaa aaagatagat
    17281 gaagatagct ttgtagtaag taatttagaa aagaaattag caatattaaa tcaggagctt
    17341 gaagttacaa aaaatagata tgaaaaaata aacaaaatta agaatgaaga agtccctaaa
    17401 ttgagtgaag aaaaaataag attgcaacaa gcaataaaat tagaagaaga gttagtttta
    17461 ttagatagag agttaaaaga tctaaaagaa agtggaatta atttaaataa gactaaagtt
    17521 gaattagaaa aagtaaagca ggtgtcagag tccagaaaag atgcagttac taaatctata
    17581 aaagaagttg aaggaaagat tgataaagta aatataagtg cagaattaaa acaaaaaata
    17641 tttctagcat atgaatacga gaaagattac aacaacgtat tggaagaaaa aaatcaaaag
    17701 ctaaataaac tagaagaaat actcaaagag acagaaaata taaatttaaa agttagatat
    17761 attgataaag ataagaatga tgtaaatcgt aatttagaaa atttatcact tcacttggat
    17821 gttcttctta aaaaatgtcc tggtaagagt gctgatttac ttttaaaatc tgaatatgtt
    17881 actgaattaa gaagtaaagc aaataatact aaagaaaatg aaattaagaa aagcagtata
    17941 caagatgaat taaagataat acttgaaagt aagtttaata ctgaaaggga agtaaattta
    18001 ctaaatgaaa aacttgaaaa taatagaaag aatagagatg acctagaaaa agaattagaa
    18061 gaactaaagt atttaaattt agcctctgaa cttagacgtg aattaaaaga aaatatgcct
    18121 tgtccagttt gtgggtcaaa acatcatgaa aatcataata taactaagta tgatgaaaat
    18181 atatcttttg tcaaagaaaa actggagaaa ttagaaaaag aaaaaataag tataagaaac
    18241 aatatagagg aattaaatgc taaagtaagt gggtatttat ctatagaaaa aatgaagact
    18301 aaagaacttg aagatgtaaa aggaaaacta ggggggattc catctagtca attattaaaa
    18361 aaacttgatg aagaacaaag aaaacttgca ttgttaaaat ctaatattca ggagtgggag
    18421 aaagaaaaag agagtactga aaataaagta acattagcta aagaagaaaa aaataaaatt
    18481 gaaaaagaag aattaaaaat aagagagagt ctgaataatt ataagaaatt aacaagagat
    18541 ttaaacatag aaatagaagg tttagaaaat aaatgtaaaa aacttaaaca ggaataccta
    18601 ggattaaaga cgattactaa agtctctaat ctattgtcaa aagtaaaaga aataagggag
    18661 aatgaaaaag aattagaatt actaagtact gggcattcaa atttattaaa aaatagagac
    18721 tcactagata ttaaaataag agaaaatgag agtaaactac atgaaataga gatagaatta
    18781 ataaaggcta gagaattata tgtagaaaag aaattgagta gagataataa gtataaagaa
    18841 gttattaaca taacgaaagg tgatttagct aaaaatcttt tacataatgt tgaagaaaat
    18901 atatgtaaga tattagaaca agaagaatca agtaagaaaa aattggaaga gcaaagatta
    18961 gaatatgaaa aaaacattgc tgaaaagcac aatatagatg gtagacttaa aactgctaag
    19021 gaacaatata aagaccaaaa atcaacacta aataaactat tagcagaaaa taaatttgaa
    19081 agtatatatg cagtaaaaag agctttatta gatactgatg cagtaaaaaa attgattgaa
    19141 gaaatactag agcatgaaga agaacaaaag ttattatcat ttaagataaa atcatcaaaa
    19201 gaaaaattaa atggaagaag cataaagaag gagtactttg aacagttaaa agatgaaatt
    19261 tataatttga aagtagaaat aggaaatata tccaaagata ttggagctaa tcaaaatcag
    19321 ttaataacac taaaagactc tttagataag ataaatgatt ttaataaaca acttaaggta
    19381 gttgaacaca atgtggactt attggaagaa ttagataaat gtgtgcaagg aaataaattt
    19441 gtagaatatg tttctacaaa ccaattaaaa tatatagcat tggaagcatc aaaacgattg
    19501 gatggaataa caaaaggaag atatgcactt gaaattgact caacactaaa ctttgtaatg
    19561 agagataact ttaatggagg agaaagaaga agcattgata ctctatctgg tggagagaca
    19621 ttcttgacat ctctatcatt agcacttgct ctttcatctc aaatacaatt aaagggaagt
    19681 gcaccattag aatttttctt tttagatgaa ggttttggtt cattagatag tgaactttta
    19741 gaaattgtta tagaatcctt ggaaagattg catagtaata atctaagtgt gggtataata
    19801 agtcatgtcg aggaacttaa aaatagagtt cctgttaagc tattagtttc atctagtgag
    19861 gctggaatag gttcaaaagt aaaaattgaa tatagttaga aaatatatgg taattgaata
    19921 tattatctaa tatttccaaa taatatgtaa ttgtgatata ataaaaacta atattaaagt
    19981 tatttataat taatattaat ataaaaacat gtaagtagga gtgtggtaag tgttggaatc
    20041 gcccaataat ttgattcaga ttattttttt aatagtatta cttatagggt ctgcattttt
    20101 ctcagcatct gaaacggcct taatgtcgtt gagtaaaatc agaatcagat atatgcaaga
    20161 tgaaggagtt aaaggagcta agttagtaag ttcattaata gaaaatccaa ataagctttt
    20221 aagttctata ttggttggaa ataacgtcgt aaatatagca gcaacatcca tatcaacatc
    20281 tttgtttata ggattaatgg gagaaaaagg agtggcactt gctacagctg ttatgacagt
    20341 attagtatta atatttggtg agattactcc aaaaacaata gcagcaaata attcagaaaa
    20401 ggtttctcta ttagtatcta aacctataaa agcaattata tttatattaa gaccaatagt
    20461 atggatattt aatataatta caaatattat atttaaatta tttggtataa caaataaagg
    20521 agctaaatca ttcataactg aagaagagct aaaaactatg gtaaatgtaa gtcatgaaga
    20581 aggcgtcctt gaaatggaag agagagaaat cataaataat gtttttgaat ttggagatat
    20641 gcaagctaaa aatgcaatgg tacaaagaat agatatggta gctatagata tggaagatag
    20701 ttatgatgag ataatacagg tatttaaaac tgaaaaatta agtagaatgc ctgtgtatga
    20761 agaaactata gatgatattg taggaatact taatataaaa gatattatat ttttatctga
    20821 tgaagaaata gagtcatttg atattaaaaa ttatatgaga gaaccttttt tcacgtatga
    20881 gttcaaaaag ataacacaac ttcttgaaga gatgaaactt gaaaaaagtc agatggctat
    20941 agttgtagat gagtatggtg gaacatctgg acttttaact atagaagatt tagtagaagt
    21001 cattgttgga gatatagagg atgaatatga tgaagaagag gatgaaatac aagttataaa
    21061 agaagatgag tatattgtag atggaagtac taaaattggt gatgttaatg aacttattgg
    21121 tgtaaacttg gaatctgagg agtttgattc cataggaggt tttataattg gacacctaag
    21181 cagattgcca gaagaaaatg aagtaattga agttgataat ataagatttt gtatagaaag
    21241 tatagaaaaa aatagaatta aaaa
//
LOCUS       DAMJVX010000055        20938 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMJVX010000055 DAMJVX010000000
VERSION     DAMJVX010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.057"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            76..897
                     /locus_tag="PC407_001805"
     CDS             76..897
                     /locus_tag="PC407_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDF2497252.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            918..2114
                     /locus_tag="PC407_001806"
     CDS             918..2114
                     /locus_tag="PC407_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDF2497253.1"
                     /translation="MFIEFFYLLRSRGLNISLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMLSR"
     gene            2280..5678
                     /locus_tag="PC407_001807"
     CDS             2280..5678
                     /locus_tag="PC407_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2497254.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNTLLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVVTVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIISFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSSINQDLETEDIGYRSINKSFKDEENRYKFIDGYNIQI
                     HNDLENEIYISKNASRQSGILSGIDVVHTPEFEEELAGLGMSQNFFKISDSLGVLSIN
                     NTDYSSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICSGLGNVNSEYAGIAEDSELIIIKLGKIDGFYNS
                     AMLFAASQYAYKKAFELRRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPHVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITVGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            5688..7361
                     /locus_tag="PC407_001808"
     CDS             5688..7361
                     /locus_tag="PC407_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2497255.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPVDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSQLNIAINRND
                     GSLSQDNIGTGTLVSGILAGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     ISGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            7635..7829
                     /locus_tag="PC407_001809"
     CDS             7635..7829
                     /locus_tag="PC407_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HDF2497256.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    7998..8231
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8308..9705
                     /gene="asnS"
                     /locus_tag="PC407_001810"
     CDS             8308..9705
                     /gene="asnS"
                     /locus_tag="PC407_001810"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDF2497257.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(9878..11053)
                     /locus_tag="PC407_001811"
     CDS             complement(9878..11053)
                     /locus_tag="PC407_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2497258.1"
                     /translation="MNLEKIKKFFGFPLDKKHKENVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFATPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCDETDAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIEKAITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            11472..12836
                     /locus_tag="PC407_001812"
     CDS             11472..12836
                     /locus_tag="PC407_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2497259.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            12858..13349
                     /locus_tag="PC407_001813"
     CDS             12858..13349
                     /locus_tag="PC407_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2497260.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            13622..14293
                     /locus_tag="PC407_001814"
     CDS             13622..14293
                     /locus_tag="PC407_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDF2497261.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            14378..15244
                     /locus_tag="PC407_001815"
     CDS             14378..15244
                     /locus_tag="PC407_001815"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDF2497262.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            15277..16779
                     /locus_tag="PC407_001816"
     CDS             15277..16779
                     /locus_tag="PC407_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF2497263.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSVVLAGLTGIPVNVLIVV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFIGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            16909..17361
                     /locus_tag="PC407_001817"
     CDS             16909..17361
                     /locus_tag="PC407_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDF2497264.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            17521..18324
                     /locus_tag="PC407_001818"
     CDS             17521..18324
                     /locus_tag="PC407_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497265.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDALYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACNECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            18382..19269
                     /locus_tag="PC407_001819"
     CDS             18382..19269
                     /locus_tag="PC407_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2497266.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEVWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNINGSSFQELASTSSLIKKVAKIKNLNEKDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            19288..20136
                     /locus_tag="PC407_001820"
     CDS             19288..20136
                     /locus_tag="PC407_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2497267.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            20199..20888
                     /locus_tag="PC407_001821"
     CDS             20199..20888
                     /locus_tag="PC407_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2497268.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKAKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITFDSSKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
BASE COUNT         8214 a         2223 c         3748 g         6753 t
ORIGIN      
        1 ttattatttt tatatcaaaa atagtaataa agtctatata catttagaaa atactgttta
       61 aaaggagtag gatttatgat aaaaaaagag atatctaact ttagaggaag tagtgattat
      121 gtagtatcac cagaattgat ggcatcagtt aatgttgcta ttgctcttga aaagccactt
      181 ttgattaagg gcgaacctgg aacaggtaaa actatgctag cacaagcaat ttcaaatgaa
      241 ttaaaaaaag atttagtaat ttggaatata aaatcaacta caaaagcaca agaagggctt
      301 tatgtatatg atactgttca aagactttat gatagccaat ttggaggaga aggtgtagat
      361 gatattagca agtacataaa atatggtaaa ctaggagagg cctttagctc aaatcaacaa
      421 gtaattcttt taatagatga aatagataag gcggatttag aatttccaaa tgacttgctt
      481 tgggaattgg ataaaatgga gttttacata aatgagacaa aagaaactgt aagagctaaa
      541 caaagaccaa tagttattat aacttcaaat gcggaaaaag aacttccaga tgctttttta
      601 agaagatgta tatttcatta tatagaattt cctgatagag acatgatgga agaaatagtt
      661 aaagtacatt ttgataaagt agaagaacac ctattggaac aagtaatgac tactttttat
      721 tggattagaa gtttaaagga catacaaaaa aagcctagta catcagagtt aatagactgg
      781 atacaagcat taactttgag tggaatgcct attgaaaaaa tagaaaaaga agttcctttt
      841 gcaggaatac tacttaagaa taatgaggat attgaatcta tgcaaagaca cttatagtta
      901 ataggagctg gataattatg tttattgaat ttttttattt attgaggtca agaggattaa
      961 atatatcttt aaatgaatgg atgactttaa ttgaagcatt ggataaaggg ctgtgctatt
     1021 caaacttttc aaatttttac tatctttgca ggatgatttt aataaaaagt gaaagtgatt
     1081 ttgataagtt tgatgcagtg tttttggagt attttaaagg tatagaacac caagaagaaa
     1141 ttccagaaga gattatgaat tggcttaaaa aaccagatat agatttagaa gagtttgaaa
     1201 gattggaaca aaatccaaat ataaatcttg ataaattaag agctaaattg gaggaacgaa
     1261 taaaagaaca agactctcct cataatggtg gtaattattg gataggcaca ggaggcactt
     1321 ccgagttagg acattcagga aaagggcaaa ctggtatacg tataggtgga aattctactt
     1381 atggtagagc tgttcttgaa gttgcaggag aaagaaaata cagagatttt agagatgatg
     1441 aagttttaaa tatgcgtcaa tttcaagttg cactaaagag acttcgtcaa ttttctacta
     1501 gaatagatgc tccaaagaca gagcttgatt tggataagac tattgaagag acttgtaata
     1561 atgcaggata tttaaagtta ttttttgaga agccaagaaa aaatacagtg aagcttttat
     1621 tgcttatgga ttcaggtggt tcaatgaggg ggtatagcac tttatgtaat acattatttc
     1681 agtctgtaag taaatcaaat cattttaaag atgtaaagat atattatttc cataactgta
     1741 tttatgatag attatttaca acacctgaat gttggttaag taaatctatt aatactgaat
     1801 ggatattaaa aaatatagat aaaaactata aagttataat tgttggagat gcttctatgt
     1861 cgccttctga gttacttcat attggtggta actatcgtgg tccatataat tacacacctg
     1921 gtatagaatg gcttaaaagg tttaagagaa agtacagtaa aattgtatgg atgaatccag
     1981 aacttagaga tggatgggat tcaattaatt attggtacca aactcaaaga atgatacaaa
     2041 gtgaatttaa tatgtttcca ctgactgtaa aaggtcttga aaaagcattg aaaaatttaa
     2101 tgctttcaag ataaagtaaa tataaatgat ggtatgattt attcaaaatt gtaccatcat
     2161 tatttatttt ttaatatatc tattataaat tgtataactg aaacttaaaa aattatatag
     2221 tctaatacat tttagactta cacaaaacat atacattaat aataatttta gaagtcgagg
     2281 tgattataat aaattatgaa ttaattgtaa aatacaatgg agatatattg agattagaag
     2341 aagaattagg tgtatcagta gagatactta attcatcata tgcaataata acatcaagta
     2401 atgaagaaga tgtaaataca cttttaactt atccagaaat tgaatttata gaaaagcctt
     2461 ttatattaca gactcaggat gtgcaaagtt tttcaagtac aggaataacg gggtttaaaa
     2521 atagaactgg tttaactgga aagggaacta taattggtat aattgattcg ggaatagatt
     2581 atactctacc tgtgtttaga gatagtgatg ggaggtctaa gatattatat tattgggacc
     2641 aatctattca aggaaatcca ccagaaggtt ttagagaagg aacattatat actaatgaag
     2701 atataaataa tgcaatagat ggaagtatgt atatacctat ttcaacaact agtttacatg
     2761 ggacacatgt tgcaggtatt tgtgcaacta ttgcaagtga tgccagaata atagtggtta
     2821 gagttggaaa tatacagacc gatatttttt cgagaagtac agagtttatg agggcaatta
     2881 aatttatttt agatagggca ttagaattaa gaatgcctgt tacattaaat ataagttatg
     2941 gaagtaatga aggttcacat agaggaacat ctttatttga acaatatata gatgatatgt
     3001 gtttattttg gaaaaacaat atagttgtag cagctggaaa taatgcagat aaaggaggtc
     3061 ataaaaggat taggcttcaa aataatatta cagaagaagt tgagtttata gttggggaag
     3121 gagagcgtat attaaacata aatatatggc cagattttgt tgatgatttt agtgtacatt
     3181 tggtaaaccc atcaaacaat caaacacaag caatctctct aacttcaggt gagataagaa
     3241 atactttggg tgaaactaga ataactggtt atttttaccc tatagcaccg tactctctta
     3301 caagaagagt tactctacaa ttaagttcaa atacacagat aactcctggg ttatggaaaa
     3361 ttgtatttga accaatagat atagtaactg gtaatgtaaa tatatatttg ccaacttctg
     3421 aaggactaaa tagaaatact agatttctga ttcctactca agagcttact gtaacagttc
     3481 ctggaacagc aagtagagtt gtaacagtag gtagttttaa ttcaagaaca gacatagtat
     3541 ctatattttc tggtgaagga gatacacaat taggtgtttt taaacctgat ttattagctc
     3601 caggtgaaga tataatatct tttttaccag gaggtacaag tggagcatta actggaacta
     3661 gcatggctac acctcatgtt acaggggttt gttcattgtt tatggaatgg ggaattgtaa
     3721 atggaaatga tttatttttg tattcacaga aattaagggc attactttta aagggagcaa
     3781 ggagattaag taatcaatct tatccaaata attcatcagg atttggtttt ttaaatttat
     3841 cagatataga tttgtacact ctatctagta taaatcaaga tttagaaacg gaggatattg
     3901 gatatagaag tattaataag tcatttaaag atgaggaaaa taggtataag tttatagatg
     3961 gttataatat acaaatacat aatgacttag agaatgaaat ttatataagt aaaaatgcaa
     4021 gtagacaatc aggaatacta tcaggaatag atgtagtcca tactcccgaa tttgaagaag
     4081 aactagctgg gttaggtatg agtcaaaatt tttttaagat aagtgattca ttaggtgtac
     4141 tatctataaa taatacagac tatagtagta tacaacgagt tttgcaactt ccttcaatca
     4201 taagaactgt atcgactaca aagatgacat tacttggaga aattaatcga ggtacttttg
     4261 gaggtgtagt tgcaacagaa gaaatgggcg taaatttctt taaaaataat cctaatatta
     4321 atataacagg aagagggact ctaatatcaa ttgcagatac aggaatagac tatctacatc
     4381 cagattttat ttatccagat ggtacttcaa aaatagtata tttatgggac caaacaaaag
     4441 aaggaactcc acctgatggt ttttatattg ggacagaata tacacgagaa gatataaatc
     4501 gagctatcgc agaaaatgac cctagtctat ctcaggatga agttggtcaa ggaactatgt
     4561 tgagtggtat atgctcaggg cttggaaatg taaatagtga atatgcaggt atagcagaag
     4621 attctgagct aataataata aaactcggta agatagatgg tttttataat agtgcaatgt
     4681 tgtttgctgc atctcagtat gcatataaaa aagcttttga acttagaaga ccactagtta
     4741 taaatatgtc tttaggtacc agtagcttgg caggtttaac caataggagc aatagtgaaa
     4801 aagcattctt tactagaggg ttatgtataa cagcaggagc aggaaatgag ggcaatacac
     4861 agacacatac atctggaata ataccacatg ttggaggttc tgttgaagtt gaattagagt
     4921 taaatgaaga cgaggaagaa ttatcattag aactttggtt aaatagacca gataaagctg
     4981 atgtaataat agtatctccc actggtgaag aaagtaaaag tgttggaatt tcaaattata
     5041 ataaagtaac aggtttattt gatttagagg ggactgaata ttctataacg tatatatacc
     5101 caacaacttt ttctgggcag caatttacta atgttacttt aaagaatgca aaaagaggtg
     5161 tatggaagat tcgattagta ggggtttata ttataacagg aagatataac ttataccttc
     5221 caaatagaga acttttaaaa agtggaacta gatttagaga ggtagaccct ttttacacta
     5281 taaattatcc tgctattcaa gatgatttaa taactgtagg agcctacaat actattaatg
     5341 gtagtttatg gcaaagttca tcaagaggac caactattga agacaggtta aaaccagata
     5401 tagtagcacc aggtgtcaat ataatagctg cttatcctgg gaacacgtat gcaactataa
     5461 ctggtacagc agcagcaagt gcacatgcag ctggtgctgc tgcaatgtat ttccaatata
     5521 cttttgttga tggtagatat ccaaaccaag catatgtgca aaaaataaaa acttttatgc
     5581 aagctggtgc tagaaaagat tcaaacacag tatatccaaa tacaaattct ggatatgggc
     5641 ttttggatgt tagaggaatg tttgatgttt taagatagga gggatttatg gaaaaatctt
     5701 attgtataat ttatcaaggt gatatagaat ctgccctaca agaaaatgga ataaataggt
     5761 atatggtttt aaatagtcag cttgcagtaa tatatgtacc tgtagatttt gatgaaacta
     5821 ttttaaataa tataatacaa gttgcttggt gggaagaatc tgaaccaatg agcagtctaa
     5881 ttgaaataac taataatgta aataatggag aaaccattac aacagcagct gaaactgatt
     5941 atatttatga aaatccatat aatgatataa caggaagagg aattttatta gcagttatag
     6001 attctggtat agattatttg catcctgatt ttataaatga tgatggaact agtaaagttc
     6061 tatatttatg ggaccaagaa gcaaatacaa atccacctcc agaaggtttt atttttggaa
     6121 gtgaatttac aagaagtcag ttaaatatag ccataaatag aaatgatgga agtttgagtc
     6181 aagataatat aggtactgga actttagttt ctggtatatt agctggaaat ggaaggataa
     6241 attcgcagta tagaggtatt actacagaat cagacttaat agtagttaaa ttaaaatcat
     6301 atacagatac ttattatgct ggaaggataa attatagtgt ttcagatttt ttagcagcta
     6361 taacgtatgt tactaatata gcaagaacag aaaataaacc actaataata aatttgacta
     6421 ttggagttaa gtcaagtgct gtagcaacta cttcaatatt agatactttt aatatattga
     6481 gtagtgctgg tgtggtagta gtgagtggtg caggaaatca agggaataca gatattcatt
     6541 attctggcag atttagcagt gtgggagaag ttcaagatgt aataatacaa gatggagatg
     6601 actatgctct tgatataaca ctaaatacga atggtccaga taaagtggga gcacagataa
     6661 tatctccttc aggtgaagtt agtcatgata taaggtattc tccagacttt tatatatata
     6721 gaggaaaatt taacttagaa aatactactt atgcaatgag atttatttat ccatatatta
     6781 cctctggaaa agaaaatttg gagataagat taagagatat aaaacctgga gtatggattt
     6841 taagattgac ttcagaactt ataataagtg gagaatatga catatactta ccaaataaaa
     6901 atttgatagc tccagacaca aggtttttag accctgattc agtagctact ataactatgt
     6961 atgctgctag tgatgatgta attacagttg gtacatttaa taataagact gatagtatgt
     7021 ggattggttc ttcgaaagga ccaattagag gaagaggaat taagccagat atagtagctt
     7081 caggtgtaga tataatatcc acatataaaa atggaacata taatacagga acaggaacag
     7141 gagtgagcag ttctatagtc acaggtgttt tagccttatt gatggaatac ttagaaaaac
     7201 aggataatgt accgagatta tcattgttta cacaagtttt aaaaacatat ttaatattgg
     7261 gtgctacaaa acttgagata tatacatatc caaatgtatc gcagggatat gggattttaa
     7321 atttaaaaaa tacgattcaa cagatagcaa atactctata gataagaacc tatgtaaaat
     7381 tataattgat tagtaattta attgtatcaa atactttaga tagagtagaa aaatggcata
     7441 aaaaataaat atgaaaatta aaaaaacagt tttatatttt gaatacacaa agtaaatatt
     7501 ttgtatataa ataggtataa attaagtatt gaaaagatat gatgttaatt taaaaatcat
     7561 tataatttag acttatgaat tttatttgta aaattgctaa aactattaca aaataaataa
     7621 tagaggtgat agttttggaa agtaaaaagg caagagcatt aaaaggtaaa gataataaaa
     7681 gacttaaaag ttcactttta gttggaaatg agtcgacagc agcatgggct gatgttgaaa
     7741 aattaaaacc tcatagtaaa gtttcaatac cttcaattaa gggtgtcgaa gaagcaaaag
     7801 actgggttga caatggaagt aaattataaa attttggaaa tttaagtaat aaagaagtaa
     7861 tgaagataag tatttattat cattatgcaa ggtagaatta ttatatttta aataacaatc
     7921 agtatcaatt atataaattt attgactaaa aatttataaa ttggtataat tttgttaatt
     7981 agtagaaata ataaaagata aatactgtga agagaagagt agatataaaa tgtagttcta
     8041 gcgagttgag gatggtgaaa gctcaatacg aggtttatat tgaagaacat cttggagttt
     8101 ctaatcgaaa gcttatttag taagtagact tagacggaag gcaccgttat aggctttaag
     8161 cattgcttaa ctaagaggtc attatgacaa ctagggtggt accgcgagag taatagtctt
     8221 tcgtcccttt atatagggat gaagggcttt ttttattgaa attatattta aaagaaatta
     8281 ataacaatta tactaggagg atttaaaatg caaaaggaat ttataacagt aaaagaattg
     8341 tacagagaaa aagaacagta catagggaag actgtaaaag tagctggatg gataagaaca
     8401 tctagagtat ctaaaaattt tggatttata gagctaaatg atggaagctt ctttaaaaat
     8461 atgcagatag tatttgatga gaaagtagaa aattttaaag aggtaggaaa attagcaata
     8521 agctcatcta tacttgtaga gggagagtta gtatctacag aaggggctaa acaaccagtc
     8581 gaaatacatg caaaaagcat agttgtagaa ggggaatcag atagttcgta tccactacaa
     8641 aagaagagac atacgcttga atatttaaga acgatagcac atttaagacc aagaagtaat
     8701 actttttcag ctgtatttag agtaagatca atagctgcct atgcaataca caaattcttc
     8761 caagaaagaa actttgtata tgcacattct ccaatcataa cgggtagtga ctgtgaaggt
     8821 gcaggagaaa tgtttagaat aacaactatg gatttaaatg atattccaaa aactgaagat
     8881 ggtaaaatag actatactca agatttcttt ggaaaagagg ctaatttaac agttagtggt
     8941 caactaaatg ccgagataat ggcactttct tttagaaacg tttatacttt tggacctaca
     9001 tttagagctg aaaactctta tacaggaaga catgcttcag aattttggat gatagagcca
     9061 gaaatagttt ttgcagacct tgaagataac atggaacttg cagaagatat gataaaatat
     9121 gttataagct atgtattaga aaatgctcct gaagaaatgg aattttttaa tagttttata
     9181 gataaaggat tgttagaaag attaaataag atagtaaact ctgagtttat tagaataacg
     9241 tatactaaag cagtggaatt attaattgaa tctggtcatg aatttgagta tcctgttgag
     9301 tggggatgtg accttcaaac tgagcatgaa agatatataa cggagcaaat atataattgt
     9361 ccagtgtttg ttacagatta tccaaaagat ataaaagctt tctatatgag aatgaatgaa
     9421 gacggtaaaa ctgttagagc aatggattta ttagttcctg gagttggaga aatcgtagga
     9481 ggttctcaaa gagaagaaag agaagatgta ttgttagata gaattaatga aatgggatta
     9541 agagaagaag attattggtg gtatttagaa cttagaaagt ttggtactgc tactcactct
     9601 ggatttggtc ttggatttga aagaataatt atgtatatga caggtatgtc taacataaga
     9661 gacgttatac ctttcccaag aactcctaaa aatgctgaat tttaataatt atatataata
     9721 aaacttatat aactttaagt ataaacacta aaatgacttg catatttatc tattttagag
     9781 atatgcaagt tatttttatt gtactataaa tatataattt gattactttt attttgcgat
     9841 ataatactta taggtttata taatagtatg taacttttta ataaaatgca acacaattac
     9901 gtccctgttc ttttgcagta tatagagcct tatcacaatt acttataaac tcatttaatg
     9961 ttattgcctt ttcaattttc tttgtataaa ctccaatact aacagttaca cattttaaag
    10021 gaacatcatc acgctcaaaa ttgcaggact ctataatttt tcgtaaacat tctgatttaa
    10081 gtttagcatc tgtctcatca cagttaaaaa tacatataaa aaattcctca ccaccatatc
    10141 gtatagcatg ttcattttca gaacttgtaa aatctaaaat tactttggca atattttgta
    10201 gacaatagtc tcccttttga tgtccataat tatcattaaa ttgtttgaaa taatctatat
    10261 ctatcatcat tccagttata ggttcttgat ttgcatggct gattaatttt ttttgtagaa
    10321 tatcttcaag gtatctacgg ttgttcattt tagtaaggtc gtctgtcata actttgtcgt
    10381 taagtaaatt atttatatga gatatttctt catattgttg actaattaaa agtttatcat
    10441 agtaagtaaa tacacgattt ctatatagca ttgtagaaac aaaaatggca agggatgtta
    10501 taaaaaatgt gttgacaata ttactaaact cattgcctgg tatagtttga atcccaggta
    10561 agaataaatt aagaaacaaa aagcatcctc caaaaactat tactgattct ccaaatttta
    10621 atactgagaa agctgcagct gctaacataa tataactaaa tacacttagg tcatttccac
    10681 ctaactggtc aagtagagta attacacaac cccataggca gaaaaaagtc acataaatta
    10741 tccataaatt tatataaaac gtacaacaat cctttttttt attccaaaga taagaaaata
    10801 caaacataaa gacagaagat acacaaaata gtgttacata catataaaaa taccattggc
    10861 gtcttggtgt ggcaaaaggg cctccttctc tagctgagat tgatatcatc ataattattt
    10921 gagatattac tattattatg caaatgattt tacccgtatg ataaagataa ttgcagagtt
    10981 ctttttcaac attttctttg tgttttttgt ctagaggaaa tccaaaaaat ttttttattt
    11041 tttctaagtt catataagcc tccattaaaa ataaagcata aaaataagta ttcctttcta
    11101 tcattagata tttggaatac ttcgtttatg atttaattat atcaaaatat attgtgtgta
    11161 acaatctaaa attgtgatag ataaaattta ttagctaatg ttgagtaact ttattaatta
    11221 atactttact ataattaaga tttacagata gtttgctgct gaaaataaag aatagaataa
    11281 atttaaatta agcataataa aatataaata ataaagttcg cattaacaaa ctatttaaat
    11341 atcaagacct gttaaaaaac aacagcaata aacaaataaa atagataaat atatccaatt
    11401 aaggtataat taagttttat atgatatagt tatatttgat aatttacaat agtaaaactt
    11461 ggagggaaaa aatgggtgaa aagaaaataa ataatatgaa ttattcacat aataattatt
    11521 ccaaaaagac aggtgaacca actagaaaaa taaaaaatag ccatatcact cttaaaagta
    11581 aagaagaaaa aatttgttct agttgcaata aagaaaataa tactaaggat aattactgta
    11641 aattttgtgg aaatgaactt tatgaaatag catcattaag accacttgaa acaaaactag
    11701 acttgaaatc taaaataaaa gagctttcat atcatgcaaa taaaagaggt gtatttttaa
    11761 caacatttac aaccatattt atattgttta ttattgcatt gatatttaaa gctattataa
    11821 ctattcaatt taatgatatt agttatttaa taaatccagc acatataatc ctagctctca
    11881 acttgggtca aattagtgta tctatgtcta ccatgatggg ttctgggttt ataaatgcca
    11941 atataggttt gttgatatta cttataatcc ctattttggt attattaata tcaaacctaa
    12001 tttttatgag aaaaagatgt agagattcaa aaactgtatt agcaaattct ctaggagttg
    12061 gaatttttta tggattagtt ttagctattt tatctatttt tactaatgta aaaactagtt
    12121 cacatagtat gttagaatat ggatatgcat tagaatatag ttatgaattt tttagtgttc
    12181 ttttaaatgg atttgtatta gggtttatat gtacatacat aactacttat aaaaaaagtt
    12241 atgaaaagga aaatatgtat ttatctcttt tttcaaattc tataagaaca ttcatattag
    12301 gttatgtttt agtattagta atactattgg tactaactat atctgatagt agttacttaa
    12361 atgaactaga tatgagtagt tattcaaatg gattaaattt atttactata ctcccacaaa
    12421 tagctagtta tatgtgggct tttgcaaatg gaatttcagt aaccataata aactcaacag
    12481 tatcaatgtt tactttaagt tcttcatctt tatttggaga tactaaatta atgttttatg
    12541 caatgggagc attgtctatg ttaatattat tattaaatgg atacaagcta agatttaagt
    12601 ataatacaga tagtataaga cctataatag tatttagtat atattatgca tttttgatgg
    12661 gaatattggc tttatttagt acatttatac tagatagtaa tattaatttc tttaatacaa
    12721 ctaattatgg aacaacttta ataatgcaat ttaaagtctt acaagctatt gtaatttcat
    12781 ttgtatatag ttttgtgata tctttaatag gatataagct aaattcagct gattaaatat
    12841 gattggaggg aatttctgtg gaaggaaata ctaataataa agtagatgat aaattttcta
    12901 aggttaagaa aaagtttggt agtggaaaat ttaaagttga attatataaa gaaaaatcaa
    12961 aattaaatag agagattgat gatattcaag aaaagaaagc aaagatattc ttggaaatgg
    13021 gaatactgac atatcaaaaa attagagaag attctattaa tgataatagt tttgataaat
    13081 tctgtgatga gttactagaa cttgataagt taatttatga aaaaaatatg gaaattaatg
    13141 aaatggaaat ggcagaaagt aatgtaactt gtgagtgtgg ttatgttgga aatgtaaatg
    13201 ataaatattg tgctgaatgt ggtaaaaaat ttgaattgga aaatgaatgt gatgatttta
    13261 taatttgtgg ttattgtgaa agtgagatag atacagaggc tgaattttgt ccatgctgtg
    13321 gaagaaaaat tataatggat ttagaataat attgggaaac ctatgtatag tatacatggg
    13381 tttttttatt gaaattattt aaatggactt ataacagtag ttatataaaa tgaaaataaa
    13441 caccaaaaaa atgaaataaa atttctttta aatgtaaaaa acaagctaat atataaaata
    13501 aatcagtaaa aatatagatg cgagaaaaaa atatgaaaaa atattgcaaa gaaaaagttt
    13561 tcatgttatt attaatacat aaatcaagaa aataaatttc agaaacgagg taatttaaaa
    13621 aatgctagat aaggtaaagg gaagattaat agtatcatgc caagctttag aaaatgaacc
    13681 tcttcatagt ccttttataa tggggagaat ggctaaggct gctatggaag gtggagctgt
    13741 tggaattaga gctcaaggag ttgaagatat tattgaaata aaaaaagtta ctggacttcc
    13801 tgttataggt attataaaga gaaactatga agattcagat atatatataa cgccaactaa
    13861 aaaagaagtt gatgaacttt taacaacagg gtgtgaaatg atagcattag atgctaccaa
    13921 tagagttaga ccaaataatg aagacttaaa agaacttata aagtatataa aagaaaacgg
    13981 tgttctagta atggcagaca tttcaaatta tgatgaggct atcaaagcac aagagtacgg
    14041 tgtagattgc gtttcaacta cactatctgg atatactcca tatactaaaa cattagaagg
    14101 tccagatttt gtattaatgg aaagattagt taaagattta gaaatacctg taatagctga
    14161 aggaaaggta aatactcctc aagatttaaa aaaggtcttt gagttgggtg ttcattcatc
    14221 agtagtaggt tcagcaataa caagaccaca attaataaca gaaaaatttg ttaaagcaat
    14281 tgaaattaat taaaaattaa tttataaatt aaaaaattat tatatttaac tatacttgaa
    14341 aattaaaatt ggaggtaaag atatgagaac aacagatatg aagggaattt attcagcatt
    14401 attagtttca tttgataagg aagggaatat aaatgaaaaa gggttaagac aaataattag
    14461 acacaatata gatgtttgta aagtagatgg tctttatgta ggaggaagta caggggaaaa
    14521 cttcatgctt tcaactgatg aaaagaaaag aatatttgaa atagctaaag atgaagtgaa
    14581 agaagaaata aaattaatag ctcaagttgg ttcagttaat ttaaaagaag ctgtagaact
    14641 tgctaaattt acaactgatt taggatatga tgcaataagt gctgttacac ctttttacta
    14701 taaatttgat tttgaagaaa taaaacacta ttataataca ataataaatt ctgtagataa
    14761 tagattaata atatactcta taccattttt aactggtgtt gatatgagtt tagaccagtt
    14821 tggagaatta tttgaaaatg aaaaaataat aggtgttaag tttacagcag cagatttcta
    14881 cttattagag agaatgagaa aaacattccc aaataaatta atatttgcag gatttgatga
    14941 aatgatgtta ccagctacag ttttaggtgt agatggggca attggttcta cattcaatgt
    15001 aaacggtgtc agagctagac aaatattcga attaactaag aatgaaaaaa tatcagaagc
    15061 acttgaagta caacatgtga ctaatgattt aataacagat atattaggaa atggcttata
    15121 tcaaacaatt aaattactac ttgaagaaca aggtgttgag gctggatact gtagacaacc
    15181 tatgaaagaa gcaactgatg aaatgaaatc aagagcaaaa gaaatataca gaaagtattt
    15241 ttaataaaat acttttagta tgggaggatt agtcaaatgg taggatttac ttggatagat
    15301 tttgcaatac tagtggttta cttattggca gttttatttg caggattgtt attttcaaaa
    15361 aaggaaatga agggaaaaga gttcttcaaa ggtgatggaa ctattccgtg gtgggttaca
    15421 tcagtatcaa tatttgccac attattaagt ccaatatcat ttttatcatt agcaggaaat
    15481 tcttatggtg gtacttggat tttgtggttt gcacaattag gtatgtttat agcaatacct
    15541 ttgacaataa aatttttctt acctctgtat agtaggttag aaatagatac agcatatgaa
    15601 tacttagaaa tgagatacga aagtaaaggt cttagagtac ttggagcttt gatgtttata
    15661 atatatcaaa taggacgtat gtctatcata atgtatcttc catcagtggt tctagctggt
    15721 cttacaggaa tacctgtaaa tgtattgata gtagttatgg gtgtaatagc aataatttat
    15781 tcatatacag gtggattaaa ggctgtcttg tggacagatt ttatacaagg tatggtatta
    15841 atagtaggtg ttacagggac attgttctat ttaatagcaa gtataaatgg tggttttgga
    15901 acagtaatgg atactcttac aactggacac aagttcttag caagtaatga agttatgttt
    15961 gataaaaata tactatctac aagtgcattt ataatattta ttggagctgg tcttaatact
    16021 ttttcatcat atgtatcgag tcaagatata gttcaaagat ttactacaac aactgatata
    16081 aagcaactta ataaaatgac atatggaaat ggtgttttat caatgggact tgcaacagta
    16141 ttttatctaa ttggaacttg tttatttata tactatactc aaaacccaga tttagctcaa
    16201 acagttcaac aagaccaagt atttgcatca tatatagctt atgaattacc agtgggtata
    16261 acaggaatac tacttgcagc gatatatgca gcttctcaat caactttatc gacagggctt
    16321 aattcagttg ctacaagttg gactttagat atacaaacta tcataacaaa agatataagt
    16381 atggaaaagc aaactaagat agcacaatat atatcattag gtgtaggtat actttctata
    16441 gctgttgcaa tagtattagc aaatggagaa ataaagtcag catatgaatg gtttaatagt
    16501 tttatggggc tagtactagg tgttttagca ggaatatttg tacttggtgc attttgtaaa
    16561 aaagcgacta aaatgggagc atatgttgga ttcatagtat cagcaatttt agttgtttat
    16621 ttaaaatacc gtatgccaga agtaacttct tgggcatatt ctctaataac aatcacgact
    16681 tctgtagtgg taggtacaat agttagtgca atagaagcta aagttaaagc aaaagtatct
    16741 ttaccaaaag ctgagactac tgtttattat gaaaaataat atttaaatga taaattgtct
    16801 tatagagtga ttagataaat aatttagtta ctctataaga ttgtataata tactttgtat
    16861 ataaaagtat aaaaatagac tttataatta tatagggaga tagtgcttat gatatttgga
    16921 aatataaatc atagtaagac atattcaaca ttgcatgaag atttacttag atgttttgaa
    16981 tatgctaaag ataattcatt aatagattat gaaaaaggta cttatacaat agatggtgat
    17041 gatatttttg tgaatattgt agagtataaa acttgtgaaa aagaagatag attctgggaa
    17101 gctcataaga agtatataga tgttcatttg atgcttgatg gatgtgaacg aatagatata
    17161 aattttatag aaaatttaga acaaaaaata tatgaagaaa agggcgattt cttgcctctt
    17221 gatggtaaaa ataatggata catggaactt agaaaaggtg attttttagt ttgttatcca
    17281 gaagatgctc atatgacagg aataaaagtt ttagaaaaag aaaatatcaa aaaagctatt
    17341 tttaaggttt tggttagata atgataattt atataaccac tagggctgaa aatagtgatt
    17401 taactttcag cgattagtga gacttcgtaa attttagtta gattactatt ttcagcctta
    17461 gttttttatg agaaaattga aaataattaa aagcttaaaa ttttgtaaag gagttaactt
    17521 atggaaaata tagatgtatt cttattaata ggacaaagca atgctagagg attgggaaat
    17581 ccaaaagaga gcatcattcc aaatgaaaat tgttttgaat atttgagtac tgatgaaatt
    17641 ataaatatga gatgtgagct agagacttca gaaggtgatg gtactatagc acctgcattt
    17701 tcaaatgaat ggaataagct tactggaaat aaagtttgtt ttattcacaa tgcaaaagat
    17761 ggttcaagaa taaaaaactg gaatcatgat aataactggt ttctaaatga cactatagaa
    17821 aaatttaata caggctgtac taccttaagt aaacattata atatagaaaa taaatacgtt
    17881 atttggattc aaggtgaaag tgatgcaaaa tatggtagtg atgcattgta ctataaagaa
    17941 agtctaaaaa aaattgcgta taggcttaaa gaagaatgta tgattgataa aatgtttgta
    18001 agtttaactg gttattggct tggagaagat gaatatttta ttagaacaag acgaattgca
    18061 gcagctcaag aatctgcttg taatgaatgt gatatactgt gtgtaggaag taaaattgct
    18121 atgcaatttc atgacagagg attaactatt gatgatgttc attacactca agaaggtctt
    18181 aatatgttag gagaggactt atgtaaaaat atttataagt atcatataac caaagaaaaa
    18241 agtgtattaa gtgatactat agacttatca gaagcaagga aatatatctg tgaattggaa
    18301 aagataagta aaagatttgt gtaatataaa attttaaata gagttgttaa tttataaaaa
    18361 taatttgtat ggagatatgt tatgaaaaat tacgtttgta tagatgttgg aggtacttca
    18421 ataaaatatg gttttattag agaagatgga tttataattg ataaatcaag tcttgatact
    18481 gaagcaaagg aaaaaggtgg agaaggaata ttagccaaaa taaaagatat agtaaaaaaa
    18541 tatatagagg aaaatgaaat aagtggtata tgtatatcaa ctgcaggtat ggttgaccca
    18601 gttgaaggta aaatactatt tgcattagaa gaattaattc caaattataa gggtatgcaa
    18661 ttaaaaaaag aagtagaaaa agagtttaat ataaaatgtg aagttgaaaa tgatgttaat
    18721 tgtgctggac ttggtgaagt gtggcttggc gcaggtagag gtgcaacatc ttctatatgt
    18781 ttaacaatag gtactggaat tggtggatgt attataataa acaataaact gataaatggg
    18841 tttagtaata gcgctggtga agtaggatat atgaatataa atggaagttc ttttcaagaa
    18901 ttggcatcta ctagcagtct aattaaaaaa gtagcaaaaa taaaaaattt gaatgaaaag
    18961 gacttaaatg gaaaaataat ttttgattta gcaaagaata atgatgaaga ttgtttaaaa
    19021 gaactcgata atatgataaa atcattagca gttggaatcg caaatatatg ttatgtaata
    19081 aatccagaag tggtaatttt aggtggtggg attatggcac aagaaaagtt cctaaaacca
    19141 aaaatagaca aagcattaaa ggaggttctt atagagagag tttacaaaaa cactaatata
    19201 gaatttgcaa agagacaaaa tgatgcagga atgcttggag cattatataa ttttttaaac
    19261 aaaatataaa gaatagaagg gtttttaatg agtattttag aacaattgga atcaccaact
    19321 tttaaagtca caaaatcaga taaattatta atagcttata taaaagaaaa tatagatgat
    19381 gtattttata aaccaatatc acaaatagct aaagagagca atattggtga agcaactata
    19441 actaggtttg taaaaaaaat gaattttaat ggattacagg attttaaagt tactttagca
    19501 caagaaatat caactatcaa taaaaaaaat attataaata aaaatataca aaatgatgag
    19561 ccagctttgg atacagcaaa aaaactttta agttcaaatg taaccacttt agagaacaca
    19621 gtagagataa tcaatagtaa ggatgtacat gactgtgcaa ggcttataat aaatgcaaag
    19681 aaagtttact tcataggcat tggatattct ggtataatag cacaggattc aaactataaa
    19741 tttatgagaa taggccttaa ttgtgtaagt tttgatagca gtcatacgat gataatgatg
    19801 tcttccataa tggaagaagg agatttaata attgcaattt cacattctgg tgaaacagaa
    19861 gagattataa aaactgtaaa gcttgcaaga gccaataatg ctaaaattat atcaattact
    19921 gaaaataaaa attctgaatt aaaggatatt tcagatgtac acttatctta tgtatcagga
    19981 gaaacagtac tggaaactgg ttcaatatct tctaaattag cacaattttt cattattgat
    20041 ttagtatata cacaggtagt aaaagaactt tctaatgaag ctattgaaag gaaaattaaa
    20101 actacaaatg ctataaaatt gtttaaaaat gaataaaata agaaatttgt aatgttttat
    20161 agagctctct taaaaattta ggaatggaga gatattacat gtctaaatta aaagattata
    20221 atattgataa agtaataatt tttaataatt taagtactaa aacgaagaat caacttaaag
    20281 aaaaagctaa actaaaaaga ttacaaaaaa aagaaatttt attttatgaa aaagatagct
    20341 tagacagtgt ttatatactt ttagaaggaa aggtatctat ttttaagata agtgaaaatg
    20401 gagagagaaa agtaattttc atattaaata gtggggaagt cataaatgat attacatttg
    20461 actcttctaa aaactccact gttggctgtg aagcatttga aaattcaatt gtagcttatt
    20521 atagtattga agaattttta gaaattatgc aaaatgattt tcaacttaca aaggatatta
    20581 tttcttatat ggaaagaaga ataagacgtt tatatagaca gttgaaaaat tctatatcca
    20641 taaaagtaga taaaaaactt gcagcaaaat tatataggtt aagtagagaa tttggagttt
    20701 gctgtggaga atggacttta ctaaatgtaa atattacagt tacatattta gctgatatgc
    20761 ttggctgtaa aagggagact gtttccagaa tgataaaggt attgcaaaaa gaagaactta
    20821 ttaagattga taataaaggt ttttatgtta aagaaactga attatcaagg tatttcaaaa
    20881 ataattaatt atgattaagt aaaagtatta ttagaatttt aattaaataa aaatatta
//
LOCUS       DAMJVX010000056        20885 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMJVX010000056 DAMJVX010000000
VERSION     DAMJVX010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20885
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.058"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(123..305)
                     /locus_tag="PC407_001822"
     CDS             complement(123..305)
                     /locus_tag="PC407_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497269.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMVKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            618..1037
                     /locus_tag="PC407_001823"
     CDS             618..1037
                     /locus_tag="PC407_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HDF2497270.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            1168..3009
                     /locus_tag="PC407_001824"
     CDS             1168..3009
                     /locus_tag="PC407_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2497271.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFVENKIGVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSIEYLNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNQSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYTIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKN
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIETEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMFMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIREIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            3036..4922
                     /locus_tag="PC407_001825"
     CDS             3036..4922
                     /locus_tag="PC407_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2497272.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKEDSDIISKIYIYLT
                     LSAISIRKYDKANNFYFSAKKVIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSKNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELRKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            4930..6930
                     /locus_tag="PC407_001826"
     CDS             4930..6930
                     /locus_tag="PC407_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2497273.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTREFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            7192..8451
                     /locus_tag="PC407_001827"
     CDS             7192..8451
                     /locus_tag="PC407_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2497274.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVEEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            8512..10299
                     /gene="iorA"
                     /locus_tag="PC407_001828"
     CDS             8512..10299
                     /gene="iorA"
                     /locus_tag="PC407_001828"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HDF2497275.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEMNDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            10301..10876
                     /locus_tag="PC407_001829"
     CDS             10301..10876
                     /locus_tag="PC407_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HDF2497276.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            10929..11999
                     /gene="buk"
                     /locus_tag="PC407_001830"
     CDS             10929..11999
                     /gene="buk"
                     /locus_tag="PC407_001830"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2497277.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            12217..12918
                     /locus_tag="PC407_001831"
     CDS             12217..12918
                     /locus_tag="PC407_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497278.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKRSN
                     EHKCKSSIVIKNEQN"
     gene            12977..13483
                     /locus_tag="PC407_001832"
     CDS             12977..13483
                     /locus_tag="PC407_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDF2497279.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEMYELLYE
                     IDNNKLNI"
     gene            13658..14680
                     /locus_tag="PC407_001833"
     CDS             13658..14680
                     /locus_tag="PC407_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2497280.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(14827..15135)
                     /locus_tag="PC407_001834"
     CDS             complement(14827..15135)
                     /locus_tag="PC407_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2497281.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(15195..15623)
                     /locus_tag="PC407_001835"
     CDS             complement(15195..15623)
                     /locus_tag="PC407_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497282.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(15846..17303)
                     /locus_tag="PC407_001836"
     CDS             complement(15846..17303)
                     /locus_tag="PC407_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2497283.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            17721..18635
                     /gene="nadA"
                     /locus_tag="PC407_001837"
     CDS             17721..18635
                     /gene="nadA"
                     /locus_tag="PC407_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDF2497284.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKLLSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            18648..19949
                     /locus_tag="PC407_001838"
     CDS             18648..19949
                     /locus_tag="PC407_001838"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDF2497285.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            19930..20766
                     /gene="nadC"
                     /locus_tag="PC407_001839"
     CDS             19930..20766
                     /gene="nadC"
                     /locus_tag="PC407_001839"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDF2497286.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
BASE COUNT         8263 a         2119 c         3666 g         6837 t
ORIGIN      
        1 ttaaaaaagc tatatataga tttttaaaga aatctatata tagcttttaa gtttatgtca
       61 atcttttttt attaatgaat aaataatata gaatacaata tacaaaaaag tttcttaaaa
      121 tgctaagata atccccattt acaaagcaac atcattatac tgtgtaaatt tgaatcaaca
      181 atgttttcac catatttcac cataaaatct tctaattgtg tgtcaataaa tgaataatat
      241 ccacttatac tatcgatgta tgcagtcatt aagtgtatac attcatcact tttatcttta
      301 tacatatata tctcccccta tatacaatat ataaatttag atactaaatc gtatcatatt
      361 aaatagttaa gtgtgtaaca tctatgcagt tatatattta tatccttata ttaataacta
      421 aagtgagttt tgacaataga ttttgggaaa ctggatgttc aaagtgtaaa cttggatttt
      481 aaaatcatta attaagatag tgtataggaa tatatgtata cataacttaa ttataaagat
      541 tataaacaat tacttttatt aactttattt aagtggtaga ataacttgga gattaataat
      601 tataaggagg atataatatg agtttataca agtttatata tgatgataag gaatatttat
      661 taaaagagga gaactgtagt gctttaataa atgatgaaga aaatccagtt caaggagtaa
      721 gtatttcaaa aataattgat atacttaatg aagcagaaga agttgatttt gatgtagaat
      781 attatcaaga agcatgtcca ttatgtttag aaggagtaaa agaaaagaaa aaattctttc
      841 cttttttaga atatcacttt tatattttca gtaaagatgg agaatatgta ataagcaata
      901 tatctgttga ttataaagga ctatctttta ataaactatc aagggcaaat aaagttgata
      961 atagttatat tgtaagtgtg ataatttgtg aaaattgtca ggattatata gttcagatag
     1021 aaaattgtat agtttagtgt aaagtatgtc ttaatttaag agaaatttct aaattgagac
     1081 atcaattttt ttcaaaaata gtatggaaaa ttactaaatt tatgagataa tgtaagtcgg
     1141 aggtgagtta caatatatag taaatatttg gaagagatag atgatacaat tttagattca
     1201 atgtttaaac taaccttgaa tgaaagatta ttgactaata acgattttgt tgaaaataaa
     1261 attggtgtag taaagaattt aattgattgc tataaaactg aacaaaatag tttaatgtta
     1321 ttaaaatcaa attttttgct agctttatta tatggtattc aagggtttag tgaaaaaatg
     1381 aaagagtata ttttgatttc ctgtaaatat attgataaat gcttacccaa agattataaa
     1441 tttttagcac gattttattc acaaatagct attttaagtt taaaaaatga agatgtaaat
     1501 aaggcaaatt tatatatgga taaatttaat cttatttgtg atgaaaataa ttttctaatt
     1561 gaagaaatta tttttaaatc acaacttatt tacattaagg caagtaagtg tatagaatct
     1621 tctgtaataa ttaaagaaat agaaacattg tatataaaga taaaagaaat taacgatttt
     1681 aactgtaaaa aaatatattt ttttattatt ggtaaaatat attttttgtt tttggatgat
     1741 gctctaattg ctaaagtaaa ttttttaaaa gcaagaaaat atgcagattt actaaaggat
     1801 atgcaagtat cttcactatg taatataaag ataggtgagt gtgaatgttc atttgaaaat
     1861 tatgaatgtt ccatagaata tttaaatgaa gtaataagca ataaaaagta tagaaatgta
     1921 aatataattc aaaaatatag agcatcaaac aacataacta aaatattaat aaagactaga
     1981 aattatcctg tagcaataaa taatttagca aaatctgaag tatatctaga aaaaattaaa
     2041 aatcagagct taaaagaagt agagaagttt gatttattta taagtttagc tatgtattat
     2101 gcaaaaagta atgaaaaatc ttttgaacaa tcaatatgct atttagatag agctaaaaac
     2161 atcttaaata tgataaaaaa tatagaagaa tatacaatat atgatttaga aataatttat
     2221 catcaggcaa agatatatta tatctttgga tattatgaca atgccttaaa cacaaataaa
     2281 aaactccttg aaaaatctaa aaatgctaaa aattataatt atgtgaagtt agcatacaag
     2341 aatatatatt tgtgttttga aaagattcaa aattatgaaa tgagtatgaa atactttaaa
     2401 atgtattatg agttaaaaat gatgtatata aaagctcgaa atagaaatta tatagattcc
     2461 ttaaattata gacatgagta tatagaaaca gaaataaata acatgagaaa agtaaaactt
     2521 gatttagata aaaaaaaata tgttgaccat ttgacaagtg catataatag aacttttttg
     2581 aacgattttt tagaaaaaca agaagttagt gaatattgca cagcatttat gatagatgta
     2641 gattacttta aagaatacaa tgatacttat ggacactata atggagatat tgctcttaaa
     2701 aatataacta taataataaa aaaatatcta aaaaaagaca tgtttatgat aaggtatggt
     2761 ggagaagaat ttctgatatt atctgtatgt aaagattata aaaaaagcaa aatatttgga
     2821 agaaaattat gtaggattgt aaagaaattt ttaaacaata atttgacagt tagtattggc
     2881 attgatacat gtaaaaatag tagtatagat ataagagaaa taattgaaaa tgctgataaa
     2941 gctttatata aagcaaagga aagtggtaga aatagatgtt tgcattatca tgattttaag
     3001 aatttttaac tgttgaaata ggattggtga tttacttgaa aaggagttta atagaagatt
     3061 taaagagaaa tataaacaag atagaatacg atatgttgac taaattctat ctaaatttga
     3121 cagatttaaa ctacatcttg gattatcaca aagacctttt aaatacaaaa ctagaagata
     3181 atagtagagt ctatacttta tatttaatta gccttataaa ctatataaaa aaagatagag
     3241 aagcatcata caactatttg atagagtcat taaagtatat taaaaaattg tcattaaaag
     3301 aagattcaga tataatatct aaaatttata tttatcttac tttatcagct atttctataa
     3361 gaaaatatga taaggcaaat aatttttatt tctcagcaaa aaaagttata agacaaaatc
     3421 atttaaatga gttgctagtc ttgttaaata tgagtttatg tgtagaaata agtgctttat
     3481 atcagaccaa aacggatgta atagctttag tagaagaagt gtcttttttt aaaactttaa
     3541 aaaataaagc tcttgtaagt agggcaaatt atatatttgg gagagtttat ctatatttat
     3601 ttaataactt tataaaaagt atggagtacc taattgaatg tctaaaactg gctcaagaaa
     3661 ataatttata ttctatagag gctttttgta aatgtataat aagtttgtgt tatttagaga
     3721 gtggaaataa tgtagaagct ataaaataca taaataatgt attagatgtt gcgcacagta
     3781 agaactcaat atcagtaact gaaagagtgt ctataaagat agaccttata tgggcatatt
     3841 taaaagaaga tatgaataaa gaagcagaag ttattttatt aaagactata aaattaatga
     3901 aagttgttga aggtgtatat aaggattatt tatattcatt tatattttta tattcagctg
     3961 aaattgagtt aaggaaagat aattgtagtt ttgaaaaggt aaatacctat ttaattttat
     4021 caaaaaatat atataaaagt tttggcgatg tttttatata taattctaaa ctagattaca
     4081 tatataagtt gtatggagat ttatatataa aatttggtaa tatagatgaa ggtataagtt
     4141 gttatataaa aggtttaaat ttagttaaaa agagttcatt gaatcttaga aaaatatcta
     4201 tattttatgg gctaatagca aatggatatg agctaaaaaa agattttaaa ctggcaatag
     4261 aatattataa aagtatggat tggtattctg ataggtggga aaaaaataat tcctataaaa
     4321 catctcaagc aattcacaaa caatatgaac tcagacaaaa acaagagagt ttaagattac
     4381 tagtggatga taataagaaa ttagaaaatg atattttaaa agatggactt actaaactat
     4441 ataatagaag atatttggaa cagcagttgg atttatttaa tggaaccaga gacaaaaata
     4501 tggttgcaat actagtcgat atagattgtt ttaaaagtta taatgataat tatggtcatc
     4561 tagcaggaga taaagttata gttaaggtaa ctgatattat aagaagtgta tttatagata
     4621 ttaccgaaca tataatcaga tatgggggag aagaaatttt aatactagta gattctatag
     4681 aatcaatcgg tgttgaagag attgataata tagaattata tattaaaaga gttttcaaac
     4741 ttcttgaatt tgagggtatt gaacatatat attctaaaat aaaaaactat ataactataa
     4801 gtgctggagt aagtataaaa agatgtcgta gtagaaaaga tgttgaaata ttaattgaag
     4861 atgcagataa gaacctatat aaatcaaaaa aagcaggtag aaatcaatac attataattt
     4921 aaaattgagg tgattttgtt gcgaaaaaaa ataggaatta tagcctctga tattgaatta
     4981 aaagaaagaa tagaagaatt atatagagaa gatgtagaaa atggaactat aataatcgac
     5041 atacttaacc tagacttaat ggaaaatcaa ggtcgtatac ttgttgaaaa aggtgcccaa
     5101 gctataattg gtagaggtgg aggttatagt ttagtaatag atacagtaaa tgtacctgtt
     5161 ataccaatga atatgaagtc aactgacctt ttaagagcaa tagaaatagc aaagaaatat
     5221 tctaaaaaag tagtattgat tcttggagac aatgaagttt cttttgacta tgttggctgg
     5281 agaaatgtca taagtactga aataactgaa gaatggtttg aatctaagta tgagataaga
     5341 agtaaagttg taaaatatat agaccaaaaa gatgaagttg taatcgtagg aggaggtttg
     5401 gcatgttctt ttgcaaggca atatggaatt gatagtgtat ttgcaactgc tagtgatgaa
     5461 tctataagag aagctgtaga atattgtaaa aaattattgg atacattagg tgaagaaaag
     5521 tttaataatg aagtgttacg aaatatactt gatggtataa aagatggagt aattgctata
     5581 gatagtaatg gtagtataat tttgtacaat gaaagtgcaa aaaacatgct taaagtagag
     5641 agaaaatgtg cacttaataa atacatctta gatgtatttc ctaaaatgga gtggatgtta
     5701 gattgtttac atgaaaaaga ggatgtagaa gatagaaaga taagaaatat aaataatcta
     5761 attgttaata ctaggacaac attaataaaa gttgataata gtacttatgg agttttagga
     5821 ataatacaag atataaccaa gctacaaaac ttagaaagaa aaataagatt tgacttgaat
     5881 caaaaaggtc tctatgctag atatactttt gatgattttt tatttaaaga taaattaact
     5941 agagaattta ttgaagaagc taaaaaaatt ggaaaaagtg attatacaac tttactttat
     6001 ggagaaagtg ggtctggtaa agaaattata gctcatagta tacacaatat aagtaaaaga
     6061 aaagataggc cttttgttgc tataaattgt gctactatag cagaaaattt acttgaaagt
     6121 gaactttttg gctatgaaga aggtgcattt actggtgcta gaaagggtgg aaaaagagga
     6181 ctatttgaac ttgctcatgg agggactctt tttttagatg aaataaatag tctatccttt
     6241 aatattcaga caaaactatt aagagtaata gaggaacgtc aaataatgag aattggttct
     6301 gattatataa tacctctgga tataaggatt atagcagcaa cgaacgaatc tttaaccgaa
     6361 aaaatagtta tgggtacatt tagagcagac ttattttata gattgagtag tttagagata
     6421 aatatacctc ctttaagaga cagaagagaa gatataatac ctttgtttaa caattttgta
     6481 aatgaagtat taaaggatga tggtctcaat ggaataaaca gtatagatga aaattttgtt
     6541 ttgactaaag atgaaatgga taagctatat aattatagct ggcctggaaa tgtaagagag
     6601 ttaaagacaa tagctcaaaa atatgttgtg acaggtaaaa ttaaattgag acaagataga
     6661 gattttaaaa ctaaaaaatt acttcctaac tctgaagtag acaaatttaa ttctgaaacg
     6721 acagcaagtg tagaagtcca agatgaatct ataaatataa gtaagataaa tgatggtaag
     6781 ataagtatag atataaaaga agtaaataag tatgttgaag aaaaaataat aagtatgctt
     6841 tttgctcagg gactttctaa aaatgaagtt gcacaagttc ttggaataag caggacatct
     6901 ttatggaaga aatacaataa aaatatttaa atataattac atataaatta ttttgaagta
     6961 atgttaattt tttaaaacaa gtgttaagaa aaattaacat tacttttttg taaattttta
     7021 tttttttaga caggcatata tatttataag aaaaatagtt aaaacactta atttaataat
     7081 gaaaaaaata ataattctaa acaaaatcaa ttttacataa agtgggattt attggcatag
     7141 taattgctta atatataatg taagagtaaa tacaatttta ggggtgatgg tatgagtcaa
     7201 agtatggttg gtaagcatgc aatgtggcca aaagaaaatg atgttatatt tagcatatct
     7261 ggtagagctc aagcagcaga gaaggctttt ggtatggata atgtaataaa tgcaacaata
     7321 ggcgctctaa tggatgattc aggaaaatta ataacaatga aaactgtata tgaagaatat
     7381 aaagcattag ataactgtga gataggtgca tatgcagctc ttgaaggtca accagactac
     7441 ttagaagcag ttaaaaaagt atttttcaga gattacttac cagaaggaca tataagagta
     7501 ttagcttcac caggaggaag tggtgcaata aaattagctg tatggaacta tactaatgaa
     7561 ggagatgagg tattaacatc tgattggttt tggagtccat atgtaagtat agctgaagag
     7621 gctaatagaa aagtagttaa ttaccaactt tttgatgaaa atagaaggtt taattttgag
     7681 tcgttcaaag aaaaatttgt aaatatagca gaaaaacaag gtagagtctt tacaattata
     7741 aacacaccag cgcataatcc aactggatat agtgttgcag atgatgagtg ggataaaata
     7801 ttagacttat caaaagaagt cgcaaaagat aaagacaaaa agataatatt tttcgtagat
     7861 tcagcatata ttgactttgc aggtgatgat gatgtatgta gaaagttttt taagaaattt
     7921 tctaatttgc ctgaaaacgt tctcgtattg gttggattca gtatgtcaaa aggatttaca
     7981 gcttatggta tgagaatggg ggctataatt tgtatatctt caagtgaaga tgtagctgaa
     8041 gagttccatt actcatgtgt tcattcatgt agagcaaatt ggtcaaattg taatagaagt
     8101 gccatggcag tattatctaa cattgtaaat gaccctaaaa aattcaaaga atatgaggat
     8161 gaaaaagaaa tatacaaaaa catgcttact agaagagcag atgtatttgt agaagaagct
     8221 gaaagagtag gattagaaat tcttccatat atagcaggtt tctttgtaag tataccatgt
     8281 gataatccaa aagaagtatg tgaagaattg actaaacata atttatttgc agttccatta
     8341 aaaatgggct taagatttgc agtttgtgca gtttctgaag ataaatgtaa gaaagcacca
     8401 agtattataa aagaagcact tgaaagttta gaagtaaaaa taaataatta atttcattga
     8461 acaaattggg tggactatga atttaataat tgtagatgga gggaatttta tatgaaacaa
     8521 ttgatgacag gtaatgaagc aattgcacgt ggagcctatg aggctggtgt taaatatgca
     8581 tctgcatatc cagggactcc aagtacagaa atattagaaa atatagcaac atataaagat
     8641 gcaatagttg cagagtgggc tccaaacgaa aaggtagcac ttgaggctgc tatagggggt
     8701 tcaattgcag gagcaagaac aatggcttct atgaaacatg ttggtttgaa tgttgcagct
     8761 gatccaatat ttacttatgc atatacaggg gtaaatggtg gtatggtact tattacagca
     8821 gatgagccgg gaatgcattc atctcaaaat gagcaagata ataggatgta tgcaaaattt
     8881 gcaaagatac ctttatttga accatcaact agtcaagaag ctaaagatat gataaaagaa
     8941 gcttttgaag ttagtgaaaa atacgataca ccagttttat atagagttac aacaagactt
     9001 tgtcattcaa aaggattagt ggagtgctat gatagagaag aagttgaaat taaagagtat
     9061 gttaaaaatg ctaagaaaat ggtaactgtt ccagcaaatg ctcaaataag aaggggcgtt
     9121 gttgaagaaa gaatggaaat ccttaaaaaa ttctcaaatg aaactgattt aaactactat
     9181 gaaatgaatg atacaaaaat aggtgttata gcttctggta tgtgttgtaa ttttgccaaa
     9241 gaagtttttg gaaaaaatgc atcatatatg aaactaggat ttacaaatcc attaccttat
     9301 gaaaaaataa aagaatttgc agaaaaagtt gataaaatat atgttataga agaaaatgac
     9361 ccatttatag aagaacaaat aaaagcatat ggaatagatt gtattggaaa agatgtaata
     9421 ccaccatatg gagaaatgac accagatgtt ttaagaaaag ctatatttgg aaaaacaaat
     9481 gatactatag aatacaaatc tgaattagta acaccaagac caccaagttt ctgtgcagga
     9541 tgtccacata gaggattctt ctatgaactt ggaaagagaa aaaatttaat agttggtgga
     9601 gatataggtt gttatactct tggatttgct cctccatata atggaataga ttatgttgtt
     9661 tgtatgggtt ctgcttttgg aactgcacat ggagctcaaa aagttcttaa tatgaaggat
     9721 gataatgaaa aaagattggt aggtgtactt ggagactcaa cattcttcca tacaggaata
     9781 aatggacttt tagatgttgt ttacaataga ggtaattcta tttcggttat attagataat
     9841 agaataactg gaatgacagg tcaccaagaa aacccaggtt cagggtatac tttacaaggt
     9901 gctaaaacta aagaagttga tatagaagga ttagttaaag cttgtggaat agaacatgta
     9961 agagttataa accctaacaa tttaaaagaa gtaaatgaag cactagattg ggctttagct
    10021 atagaagatg aaccatcagt aataataaca agatggccat gtgtacttaa aaaattctct
    10081 aaagaagata tagaagaatt taataatcca tttaagacta agtgtaaagt tgaccatgat
    10141 aaatgtatag gatgtaaatt atgtttgaaa actggatgtc cagctctatc atttgataaa
    10201 gagaataagt tatcaaatat agatagaaat cagtgtgtag gatgtggagt atgtgcacaa
    10261 gtttgtccaa aacaagcaat agttaaggag gaaaaataat atgactaaga gtatactttt
    10321 ggtaggtgta ggaggtcaag gaactatact tgctagtaaa ttattaacta tgggattgat
    10381 ggaagctgga tatgatgtaa agatgagtga aatacatgga atgagtcaaa gaggaggttc
    10441 agtttcctca caagttagat atggagattg tgttcattct ccagttatag aaattggtgg
    10501 agcagatata ttagtatctt ttgaaaaaat ggaagcttta agatggttca attatttaaa
    10561 accagaagga aaagctttag taaataatca taggatagat tctatgactg ttttaatcgg
    10621 aggagctgaa tatcaagaaa gtgatataga tgcagagtta agtagattaa atgcaaaagt
    10681 tataaatgca gctgataaag ctgaagaact aggaaattca aaaataatga atgttatttt
    10741 attaggatgt ctagttaaat ctatgcaatt agagtctatt gattgggaaa aaattataag
    10801 tgaaaatgtt aaaccaaaat ttgttgaatt aaacataaaa gctttccatg aaggtatgga
    10861 aatggttagc aaatagttta taatagatat aaatagacta tagaaattaa ggtgaaagga
    10921 agaaaattat gacttacaga atattagcca taaatccagg ttctacttct acaaaaatag
    10981 cagtatatga tggagaagaa caaattcttg tgaagacgat agaccatcca gctgaagaga
    11041 ttgcaaaata taatactata caagaccagt ttgaaatgcg taaggaagca gttttgaata
    11101 ttcttaaaga aaatagtata gacttaaaat ctcttagtgc aatagtagga agaggtggag
    11161 ttttaccacc agtaaaatca ggagcatatc tagtaaatga agaaatgatt gatgtactaa
    11221 gacatagacc agtacttgaa cacgcttcca atttaggtgc tgttgtggca catgcaatat
    11281 cagaacctct tggaatcaac tcatatattt atgattctgt tgcagtagat gagcttatag
    11341 atgtagcgag aatatctgga ctttgtggaa tggatagatc aagtgcaggg catgcattaa
    11401 atactagagc aatggcttta aaatatgcta aggataaagg aaaagattat aagagcttaa
    11461 acttaatagt agctcacatt ggtggaggag taagtattta tcttcatgaa aaaggaagaa
    11521 tggttgatat gctatctgat gatgaaggac cattttctcc agaaaggtca ggaagagtac
    11581 ctgctacaaa attagtggct gcctgttatt caggtcaata ttcagaaaga gaaatgacta
    11641 aaaagataag aggtaaaggt ggtatagttt catacctaaa tactgtagat gctagagaag
    11701 ttgaaaaaat gatagcagaa ggaaatgaag aagcaaaaat tatttatgaa gcaatggctt
    11761 atcagttagc aaaaggtatt ggagagttag caactgtagt agatggaaag gtagatgcta
    11821 taattataac aggtggaatt gcatattctg aaatgtttac ttcaatggtt aaaaagaaag
    11881 ttgagtttat agcaccagta gaaattatgg caggagaaaa tgagttggaa tcacttgctt
    11941 ttggaacttt aagagtacta aatggagaag aagaagctag aatttatagt gaaaattaat
    12001 aagaaaaatt aaaaatgttt atatactaaa aaagtggtat atccgttttt tatacacgaa
    12061 agcttagagt ataggtgaaa atgtataaaa tttggattgc cactttttat aattaaaatt
    12121 ttatcttaat ctaataaata attctgtaaa atttcgtatt tatatatata ttttattggt
    12181 atttattgtt attattatta tgaggagtga tttttgatgg gaaaaataag tttaaaaagt
    12241 atgataagat tattttcagg tttttttata tttgcaattt cttctgtgct tatgattaat
    12301 gcacatgtag gtcttatgcc ttgggatgta cttcaccaag gtttatctat aaagctaggc
    12361 ataactatag gtcaggcttc tataatggta ggagttgtaa ttgttatatt ggatgcagtt
    12421 tttggtgaaa atataggatg gggtacttta ctcaacatga catttatagg aatttttatt
    12481 gatttagtaa tattttctgg agtgatacct catgcaagca acacttatat aggtgtgttt
    12541 atggttgtta taggaattat tttggcagct atagcaagtt ttctatactt aggtgtttgt
    12601 ttaggaagtg gaccgagaga tgggcttatg attgctcttc agaaaaaaac taataaatct
    12661 gtacgtcttg ttagaacaat tcttgaaata ctagcattag ttgttgggtg gttattagga
    12721 ggttctgtag gtattggtac gcttgtaagt gctttaggtt taggatatgt attacaaata
    12781 gtgtttagaa tatttaaatt tgacacaaag ttgctgaaac atagatttat tatagatgat
    12841 ataagagaat ggaaggaaaa gagaagcaat gaacataaat gtaaatcaag tattgtaata
    12901 aaaaatgagc agaattaatt atgctaaaat atatttgtag tttatctaat ttatagtaaa
    12961 gtcaggagat tttgaaatgg aattatggga tttatataat gctgatggaa ttaaaacagg
    13021 aaatgttata aaaagaggta attctattga agaaggttat taccatttgg cagttgaagt
    13081 gtggatttta aatagtaact ctcaaatttt aattcagaaa aggtctaaaa gtaaaaagac
    13141 attgccaaat atgtggggaa tgacaactgg atgtattgtt tctggggaag atagtttaga
    13201 aggagcaatt cgcgaagcaa aggaagaaat agggatagat ataactaaag atgagatgaa
    13261 agtttttaga agtatgattc atgaagatac tctttgggat gtatacttag ttaaaaaaga
    13321 atatgatata tccaaagcta ttttacagga ggaagaagta agtgatataa agtgggtaag
    13381 cacagatgaa ataagacaat tactaaaaga gggtttgttt tttgaatatc cagagatgta
    13441 cgaattgcta tatgagatag ataataataa attaaatata tgatgtagag cagatttcag
    13501 tataagtaag attatttaaa atgtatcttc tctaaatttt aatattgttt tatgaaaatt
    13561 aataagttat ttttagtatt tttctacttg ttttttgaga ttaaaataaa aagggcagtc
    13621 attataaagt cataaaaatt tggggaggat ttaaactatg gaagataaca aaggattggc
    13681 acgtgatagt tcaaaatata aaattgtttc tatatctgtc gtagtaatta ttctaattac
    13741 attttggtat atgttaaata tggtgctttt aacatttatt atgactttta ttttctataa
    13801 cttgcttgta gccacaagaa aacgaattaa gaagttttct tctttaaata ttccagattc
    13861 attgataatt atagtgttat atgcactatt tgctatattg ttagtattga ttagctatgc
    13921 agttgtacca ataattatag ttcaacttac agagctgagt agggttttta gtgattttga
    13981 tgtaaatcaa tttgctcaat ctttaggacc aaaattgtat cctattgtct caaagctgga
    14041 ttttaataaa tatatttcac aagcagggct acttatagca tcaacagcaa caaaagttgg
    14101 aagttttgga gtaaatatat tattggcatt tttactgagt ttactccttt tacttgaaaa
    14161 aaatgaaatt aaaaattttg gagataaact ttctgatagt aagatttctt ttatttataa
    14221 ttcgcttgta ttttttggaa agagctttgt taaaaatttt ggtgaagtaa tgaaagtaca
    14281 agttatgatt gcctttataa attctgttgt ttctatgatt ttcttaggtt tcatgggatt
    14341 ccctcaaata tgggctttag gatttatgat ttttgtgttg ggtcttattc cagtagcagg
    14401 agttatagtg tctttgattc cattaactgt aatagcattt aatacaggag gaataacaaa
    14461 ggtttttgga gttttactta tgatttgtat agtgcatgca gtagaaacat acatattaaa
    14521 ccctaagttg atgtcgaata gaactaaact tccagtttgt tttgttttta ttattttact
    14581 tgtaggagaa cattatttag gtgtatgggg attactaatt ggagttccaa tatttatgtt
    14641 ccttatggat attctgggtg ttaaatttac ttctaggtaa tatattaaaa taataattta
    14701 tttttatata taaattatga attgttttaa aaaagaacct ataaatagac atcattaata
    14761 aagacatcta tttgataggt tcaattttgc tttgtataaa tactatagaa ttaaataaaa
    14821 agaaatttat ttcatttcaa aagtttcaca acttgcattt ctttcattta tatgaacttt
    14881 ttcagctttg caaagttcat ttttattgta tttacatttt acagcttcac aatgaatgtt
    14941 atctgtatca acttggttag tttcattaga acgactagtt ataccagatg tatatttgtc
    15001 ttcaaaagaa gcacaataag tattttctgt agtagttgct tgcattccat ctacttttat
    15061 actaccagca taacaatatc cactagtatt ataagcacaa ttagtagcag cacaatttaa
    15121 attattattt ttcataaata acctcctaag ttttatttaa acttattatg tgaagttatc
    15181 taaattttat acacttattt acaattacat ttatcacatt taacaactac atctttggaa
    15241 gtttcccttc ctaaattttt attaacatga tacacatcaa cattttcagt tgatccacat
    15301 atagaacatt taaaattgtt ttctagtagg gttctttctt taaataactt ccaatacata
    15361 gaattgatgt aatcattata atttaccttg aggcatgtct caagaaagtt tatgtataga
    15421 ttgtctaaat ctcctgtatt taattctgta tattctggtt caaaatattc acaacatcct
    15481 ctaaaatcag gctttgtatc tgaaataaca catctaatat catatctata gtctaggcta
    15541 ggccccttaa atgaaagatg tgtgcaaaaa gaacaagatg tttctggtag atttttttca
    15601 agttcatgta atttattatt cataagtatt cctccaaaaa atttatttta agtatataaa
    15661 tgaatagatt attttaaaac ttttatttat ttatattaga gatttaggat aaatattcca
    15721 ataaggagta aatacttaag tatttattac tgtattaaat aaaaaagagg caaatctaac
    15781 ttgagattgc ctactttttt atcatttttt aataaaattt attctaagta ttattttatt
    15841 tagatttagt cagccaattt agtatccgct ttgcttttta actttctacc agagcgtaca
    15901 gttaaaatag catataatac agtaaatatg gcagctaaaa catatgatat atttatgttt
    15961 aagttaaatc caatttgagc attaaatata taactgaata taacaaaaga ataaaacata
    16021 ccaggaatta agcttataat ataattcttt tcttttatta taaggtaaac tgtaatcatt
    16081 gcaaatgcaa atatggcaat agtttgatta ctccaagaga agtatctcca caatatatta
    16141 aatccagaag cactaagctt tgcaaatata agaatggcta tgacaggaat aaatatacag
    16201 attgacaata taacacgatg tttcttttcc ttttggtcat aatgaaagta atctgcaagc
    16261 attaatctta aagatcttaa agctgtatct ccagaagtta ttggtaaaac aattacacct
    16321 attatagcta tgataccacc aatagaacca agtaaatctc ttgcaaccaa acctattaca
    16381 tcaggagaac ctactaattc cttaggtatt cctttattgt aaatacccat agcagctgca
    16441 gcccaaatca ttgcaattaa accttctaaa atcatcatat tataaaaagt agtttttcct
    16501 tctttttcat ttgaaactga tcttgctata agagtagctt gagttgagtg gaatccagaa
    16561 acgataccac aagcaactgt aacaaagaat gtaggaataa gaggaatacc atcaggatga
    16621 atacctttcc aatttgcaag acttaaatta gctaaatcat aaccttgtgt aaagattcca
    16681 attccaacac cagcagcaga taaaagtaga atagctccaa atattggata tacctttcct
    16741 atgattttat caattggaaa gagtgttgca cacaagtaat atagaaagat aagtccataa
    16801 actatccaga caactggatt atttatagtc gatttcatat ttaaaatttg agtaacaact
    16861 aaatccccag gtgtataaat aaaaactgca ccaactaaaa gcattaaaag acaaaggaat
    16921 atattataga cttgaaatac tttattacct aaaaatctac tgataagaga aggcatttgt
    16981 gctccttttt ctcttatact aatcattcca ctcatatagt catgcaaagc tcctccaaat
    17041 acacagccaa taggtattag tatgaatgct attggaccaa acaatatacc ttggataggt
    17101 cctagaattg gtcctgtacc agctatatta agcaactcaa ttagactatt tttccatttt
    17161 ttcattggga catagtctac tccatcactt tgagcaagtg caggtgtttt tctgtcatct
    17221 gggccaaata ctttttcaca ataagcaccg tataaagcac ctcctattag taatatagcc
    17281 aagccgatta agaatgttgt cataatatca ccccatttat aaaatttttt ggtctttgta
    17341 tacatattat ctaaatgtgt agatttatat tgaatttaat tctggacaac ctacaaattc
    17401 tataaagtat tacaactaat ataatttcag aaataaatac atatacataa ccatgtactt
    17461 agtatacagg tatttggtaa tattttcaat gttatagcaa taaagaatat aaacaaaatt
    17521 ctgataatat tttacaaaaa aattaaaaaa tataaaaatt agtttaaata gatggagaaa
    17581 aaaggaaaat caattgagaa tataaatgtc tatatcttaa caatattaag aggtttaaaa
    17641 ttcttgaaga aagaatagtc aagtgatata atgggtttac aggtgtatat acactagtac
    17701 atatagaaag cggtgcaatt atggataaag atttaacata tcaaatcaaa gagctaaaga
    17761 aagaaaaaaa tgcaataata cttgcccatt tttatcaacc accagaaata caagaattag
    17821 cagatgcagt tggtgactca tattatttaa gtgagattgc tagagattgt aaagaagaag
    17881 tggttgtatt ttgtggagta aggtttatgg gagaaagtgc taagctatta tcacctgaaa
    17941 aaactgtatt gatgccagta agtaacgctg ggtgtgctat ggcagatatg gtagatgaag
    18001 aaggcgtgat aaaattaaaa caacagtatc caaatgcttt ggttgtatgt tatataaact
    18061 ctacagctaa agttaaggca cattgtgatg tttctgttac atcttctagt gcaattaaaa
    18121 tactagaaaa catagataat aaagaaatta tattccttcc agataaaaat ttaggaggat
    18181 atattgcaga acaatttcca gataaaaact ttatattttg ggacggatat tgtaaatacc
    18241 ataacaatat aagagctgaa gaaataattg aattaaaaga taagtataaa aatgcagaag
    18301 tattagtaca tccagaatgt aaaaaagaaa taagagattt aggagattat gtaggtagta
    18361 catccggaat aataaaatat gctactaata gtaaaaacaa agactttata atagcaactg
    18421 aagaaggtat attacatgaa ttaaagaaaa ataatcctaa taaaaacttt tatattcctg
    18481 gtggaaaaat actatgtaca gatatgaaaa agacaacatt agaaaattta tatagtacac
    18541 ttaaaaatat ggaaaatgaa gttattgtag aagatgaaat catggaaaag gctttaaatt
    18601 cactgcttaa catgcacaaa ttagcagaag gatagaggga tagacttatg aatttagaac
    18661 aagatgtttt aatagttgga agtggagttt caggattata ctgtgcatta aatttagata
    18721 aaagtttaaa tgtgctagta gtatcgaaat caacaattga aaacaacaat acatatcttg
    18781 cacaaggtgg aatttcaact gctagaaata ttgatgatat agaatccttt gtagaagata
    18841 caatgaaggc tgggcagtat aaaaatagag ttgaagcagt tcaagtactt gcagatgaat
    18901 ctatagaaaa tgtagggcag atagtagaat atggtatgcc tttagacaaa gaaaatggag
    18961 agatagatta cacaagagaa ggagcacata gtgtaaatcg aatagttcat agtaaggata
    19021 atactggaga agttgttttt aaaactttat taaaagaagc taaaactaga gaaaatataa
    19081 ctcttataga agatgcatat cttttagata tattaaaaga tggcaataaa tgtataggag
    19141 ctagaatttt taaatctaaa aaagagattc atgttttttc aaaaatagta gttttagcta
    19201 caggtggaat tggtgggctt tttaaaaatt ctactaatca aagacatctt acaggtgatg
    19261 gaattgcaat agctttaaga aataatataa aaatagaaaa tttagaatat attcaaatac
    19321 atcctacagc cttttatgaa gaaaataatg aaggtaggag aatgcttata tctgaatcat
    19381 taagaggaga gggagctaaa cttttaaata aaaataaaga aagatttgtg gatgaacttc
    19441 ttcctagaga tgtagtttca aaagctattt ttgagcaaat ggaaaaagat aaattacctt
    19501 atgtgtattt agatgcaaca catttagata gtgaatattt aattaataga ttttctttta
    19561 tttataatga atgtttagct agaggaacag atataacgaa agaatgtata aaggtatctc
    19621 ctgcacaaca ttattttatg ggcgggatac atgtagattt ggattcaaaa acatctatgg
    19681 aaaatttata tgcagttggt gaaattagtt gtacaggtgt tcatggagcc aatagattgg
    19741 caagtaattc attacttgaa gggcttgtat tttcaaaaag agcagctaaa aatataaact
    19801 cagttataga taatgtaaaa gttaagttta tagatgtacc agatatggat atagatatag
    19861 aacaggtaaa aaaagaaaat aaaatattag tcataaaaga aattgaaaga acaagtgagg
    19921 attttggcga tgaattattt gattattgat agaatgatac aggatgcttt gatagaagat
    19981 gttccaagtg aagatataac aactaattct attgtggatg aaaattctaa atctactgta
    20041 gatttaattt gcaaacaaga tggtataata gctggcttag gtgtatttaa gagagttttt
    20101 gagatacttg gtgatgtaga tgtaaaatta tataaaaatg atggtgatag agtaaagaat
    20161 cgtgaaaaga tagctgtatt aacaggtagt actagaaact tacttgtagg agagcgtgta
    20221 gcattgaatt atttacagag aatgagtggt atagctacta ttacaagtag gtatgttgaa
    20281 aagcttgaag gtacaaatac aaaattatta gatagtagaa aaacgatacc taatcttaga
    20341 atattagata agtattctgt taaagtaggt ggaggatgca atcatagatt taatttatct
    20401 gatggaatat tgcttaaaga caaccatatt ggggctgctg gtagtgttaa gaaggcagtt
    20461 gaattagcta gaaaaaatac ttcttttgtt agaaagatag aagtggaagt ggaaacttta
    20521 gacatggtaa aagaagcaat agaagctaat gcagatataa taatgttgga taatatgagt
    20581 ttggaaatgg caaaacaagc agtagatatt ataaatggaa gagccattgt agaattttcc
    20641 ggaaatgtaa atttagatac tatagaagat ataggaaaaa ttggtgtgga tgttgtttct
    20701 gtaggagcgc ttacacattc tgttaaagct ctcgatataa gtatgaagaa tttgagaaat
    20761 atataagttt tatattgaaa taaacttaat taaaatagac tccttaaatt tgttttataa
    20821 atttaaggag tctatttttt attcattaaa attaataaaa atataaaaaa atcaatcaat
    20881 aattt
//
LOCUS       DAMJVX010000057        20807 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMJVX010000057 DAMJVX010000000
VERSION     DAMJVX010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20807
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.059"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            229..619
                     /locus_tag="PC407_001840"
                     /pseudo
     CDS             229..619
                     /locus_tag="PC407_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            939..1448
                     /gene="vanZ1"
                     /locus_tag="PC407_001841"
     CDS             939..1448
                     /gene="vanZ1"
                     /locus_tag="PC407_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HDF2497287.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1550..1744)
                     /locus_tag="PC407_001842"
     CDS             complement(1550..1744)
                     /locus_tag="PC407_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HDF2497288.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIREFQDTIIL"
     gene            complement(2259..2651)
                     /locus_tag="PC407_001843"
     CDS             complement(2259..2651)
                     /locus_tag="PC407_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2497289.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
     gene            3219..3581
                     /locus_tag="PC407_001844"
     CDS             3219..3581
                     /locus_tag="PC407_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497290.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            3565..4281
                     /locus_tag="PC407_001845"
     CDS             3565..4281
                     /locus_tag="PC407_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497291.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVVEFTEEDEEYEEELKQNIIE
                     NIKKNFDEKKLVQVYKYRGV"
     gene            4301..4720
                     /locus_tag="PC407_001846"
     CDS             4301..4720
                     /locus_tag="PC407_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497292.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            4710..5234
                     /locus_tag="PC407_001847"
     CDS             4710..5234
                     /locus_tag="PC407_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497293.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYVDENNNEESISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVLITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            5546..6103
                     /gene="efp"
                     /locus_tag="PC407_001848"
     CDS             5546..6103
                     /gene="efp"
                     /locus_tag="PC407_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDF2497294.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            6195..6650
                     /locus_tag="PC407_001849"
     CDS             6195..6650
                     /locus_tag="PC407_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2497295.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCPSCDDRE"
     gene            6813..7523
                     /locus_tag="PC407_001850"
     CDS             6813..7523
                     /locus_tag="PC407_001850"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDF2497296.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            7577..8647
                     /locus_tag="PC407_001851"
     CDS             7577..8647
                     /locus_tag="PC407_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2497297.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            8675..12229
                     /gene="smc"
                     /locus_tag="PC407_001852"
     CDS             8675..12229
                     /gene="smc"
                     /locus_tag="PC407_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDF2497298.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKILEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEASKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKVSLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEAYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSIATEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIGSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            12256..13536
                     /gene="ftsY"
                     /locus_tag="PC407_001853"
     CDS             12256..13536
                     /gene="ftsY"
                     /locus_tag="PC407_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDF2497299.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDSLENLEETIFSG
                     LEEEVIDKVEDIEEKTEESVNNEGTEEIENFEKIEVEMDSNAKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            13639..14007
                     /locus_tag="PC407_001854"
     CDS             13639..14007
                     /locus_tag="PC407_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDF2497300.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            14004..15356
                     /gene="ffh"
                     /locus_tag="PC407_001855"
     CDS             14004..15356
                     /gene="ffh"
                     /locus_tag="PC407_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDF2497301.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMAELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            15394..15666
                     /gene="rpsP"
                     /locus_tag="PC407_001856"
     CDS             15394..15666
                     /gene="rpsP"
                     /locus_tag="PC407_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDF2497302.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            15731..15958
                     /locus_tag="PC407_001857"
     CDS             15731..15958
                     /locus_tag="PC407_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDF2497303.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            16066..16581
                     /gene="rimM"
                     /locus_tag="PC407_001858"
     CDS             16066..16581
                     /gene="rimM"
                     /locus_tag="PC407_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HDF2497304.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            16590..17285
                     /gene="trmD"
                     /locus_tag="PC407_001859"
     CDS             16590..17285
                     /gene="trmD"
                     /locus_tag="PC407_001859"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDF2497305.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVYIYNIRDFSNN
                     KHKKVDDYPFGGGVGMVMTPQPIYDTYKHIITTHNISNPSVIYLTPKGKVYNQSIAKQ
                     MSLKEDIILLCGHYEGVDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            17470..17820
                     /gene="rplS"
                     /locus_tag="PC407_001860"
     CDS             17470..17820
                     /gene="rplS"
                     /locus_tag="PC407_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDF2497306.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            18141..19037
                     /gene="ylqF"
                     /locus_tag="PC407_001861"
     CDS             18141..19037
                     /gene="ylqF"
                     /locus_tag="PC407_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDF2497307.1"
                     /translation="MSNEYEEYLMDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINNIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIAATV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            19426..20718
                     /gene="brnQ"
                     /locus_tag="PC407_001862"
     CDS             19426..20718
                     /gene="brnQ"
                     /locus_tag="PC407_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2497308.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFALSYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVDVPVV
                     KSLPLHSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         8742 a         1943 c         3739 g         6383 t
ORIGIN      
        1 aaaacgaact aaaattatca cactaaagca taaaacatac tctaatcatg ttagataaat
       61 ttttgaatat ataatccaaa agtactctct aaagtagagt acttcttttt tattaattat
      121 ttttatatgg aataaattgg aatattatat ttgttccaat ctgattgatt acatactatt
      181 attagtataa aatttaatta tacataaatt ggtaagaggt gtaaactaat gaagattaat
      241 aaattaccac atgcagaact aaaactaatg aagtatattt ggggagcaga tgatgtatta
      301 gcctcaagag atatcataga agcaatgaag ttgaaatacg attggaaaaa gagcacaaca
      361 ttgacatttt taaaaacttt agtagataag ggatttttaa ctacttatta aggtagatag
      421 atgtacgcat tatactatag ctataaaaga gaaagattac ttaaaagttg agacaaagag
      481 tttttttagt tttatgcata acaattcatt taagagtttt atttcagcac tacatgatga
      541 tgaagtttta gatagtaaga gcttggacaa attagaagaa tactttaaaa atttgaaaga
      601 agaagatatt gatgactaat aaagctatca gtatcttttt agtttgagac aattgagagt
      661 agcgtttgga tttaatgtta gttttatgtg atggtaaaac attatataaa gaaaagtgat
      721 tatttcctta gtgtttgata gtaaaaaata tagagaataa taccaattaa aatttaaaga
      781 tatatgaaat actacaatag cttaaatcat gtaaatatga taaagatatt gaaattataa
      841 aaggaatata attttagaag aaatagttga cagataaatt accttactat attattgtat
      901 taataaaata atacaataat atagtaagga ggtaataaat gaaatctaga aaacataata
      961 ttacaaaagg attatttata gtttacataa ttattttaac atggataata ctatttaagt
     1021 tgcaatttga tatctcatct cttgaaacca tgaatcttag aagcataaac ttagtacctt
     1081 ttgctgggtc acttataatt aataatagag tagatatatc agagattata ttaaatgtgg
     1141 ctatatttgt accatttggg atatatgtgt gtatgctaaa agaagaatgg agctttataa
     1201 aaaaagttat acctattttt ataactagtt tagcatttga aacacttcag tatatatttg
     1261 cactaggtgc aagtgatata accgatttga taggaaacac actaggagga atcataggaa
     1321 tagctgtttt tatgctatta tcgaaaatat ttaagaataa taccataaaa attatcaatg
     1381 tattggcatt gattgtgaca attattgttg tattattttt aggattagtt atttttgcaa
     1441 acttatagaa ataagtatat aggtttagct taaaatagaa cgtaagatac agttacatat
     1501 aaaattattt acacataaaa agcacctata taggtgcttt cctaaattat taaagaatta
     1561 ttgtatcttg aaactctcgt atgttcaatt cttgattttc tcttgttttt tctaataaat
     1621 ctaatgcaca atttatacaa actttaattt ctgcattggt atttttctca taataagtta
     1681 tatatttatt tctatattta tctatttctt ttccacaacg actacacatt acaattacgc
     1741 tcatatctta gttcctccat ctctaattat tcttaatata ttcaatagta tcacttttct
     1801 gaaaataatg attgttaaaa ctgtatcaaa tttagattta ctttttatta taatgcattt
     1861 taataaaaaa ataaaggtta tgggtaaaat atttatagaa aaaagtatat tattgaatag
     1921 attataaaat tttattattt ttcatcagtc ttaaatacac attaatatct ataaaatgac
     1981 aaaataaaaa atactattta aatagagatt aaaaaattga tgcgaagtat ttgtttgcta
     2041 tatgaataac aatttattta gaagttttga tttgacactg tataataata aagtaattgc
     2101 taaaaaagtt tgatgatttg gatgtatttt tagaaattag aaaaatatat acttactgac
     2161 taatgataat cttatatata ttttagtttg agtgatttaa gaaaagtgtt ttgaaagggg
     2221 attattttat agaataaata tatatatata gtagaaaatt acatataaag agtagtattt
     2281 atttcattac cttctttatc tgttccgatg gggtcttgta gagatacttg agttttattt
     2341 tttttgttag atctaatatt cattaaaatc tcattttcaa tacatctaga agcatatgtt
     2401 gctaatcgag ttcccttatc tatgttataa gtttctatag ctttaattaa tcctattgta
     2461 cctatggata ttaaatcatc ctgatcttcc gttgaattat tatatttctt tgctatataa
     2521 gctactaatc tcatgtttct ttcaattaat gtatcttttg cacttttatc gttttctatt
     2581 ttaaatttag ttaaatactc aatctcttct tcaggggtta agggtttttc aaaagattta
     2641 agagctgcca ataggtcacc cccttttcaa cggtcacttt aaattatatg aaggagactt
     2701 atccaagttg actgttatct aataaaataa tatgcatatt tttagaattt aaaacatata
     2761 tttgcaaact tttgaatgga atatgacata aagagtggga atttaaattt aagtttttgt
     2821 tgtatatata tgaaacaagg cgaagaaatt attgatttat aaagtataaa ttggaattga
     2881 ggtgtcaata aatataaaag gaaatagtat ttatatatgt tttggtgttg aggtaaaaga
     2941 ttaatgcaaa gcattagagt ctataaaaat atgaatatag gaggtgtaat tttacaatac
     3001 ttaaaaaagt aaatgtaatt aattgtttgt ataaagttga ttacaagcaa aaagtatcag
     3061 tcatagttaa ttctgttaag gatgaaggtg gtgtgttaat ttatatatgt atgggaagaa
     3121 attttgatta caatgaaaaa acttacaaaa gatatttatg aaaaaactta tagttaaatg
     3181 tttatatata aaatattatt taggggttgt ttttattatt gaaaataaga aagagtggtt
     3241 ttatatcaat cgaatgtata ataagtattg ctatattata tgtggctgtt tatttagttt
     3301 ctacatcatt gtataattgt tatagtttta tcagtagaaa tatatctgac agagaaatgt
     3361 taagtacagc aaaaaaatat atagaagatg agaagtatag aatacaaaat agtaagtatg
     3421 agttaattga agataagata gaaaaaaatt acataaatgg atatgaaatt aacagtagaa
     3481 tagagcaaat tttagattat tatcaatgct atgaaataaa tatagagata aaaaatgaat
     3541 ttaaaaaact gaggtttaat agctatgtta ctagaaaata aaggctctat attaataata
     3601 acattgataa tattttctat tataagtact gtgtgcatta tgtgtatagg tttaatttat
     3661 acacataata atatgtttga gttaggatac aaggacattc aactaaaaga aatttcttta
     3721 agtggaatag aaatatcagt aagtaatgta ctttcttgtg ttgaggaagc tattgatgta
     3781 tctaataatg aagacgaatt tagagaatat ttcttaggaa ataatatgat tgaatgtata
     3841 aaaaaaataa aagatacatc ttatagtagt ttagaagaac tttctttaaa agtagaaaat
     3901 aatactattt atgacagagg agattttttt gaatttgata tagaaagtaa tgttagagaa
     3961 aaagaatttt taaaagcaat aaaagtaaga gtgaaaatca aaaatccatg gataggaatt
     4021 gacattgaag attatatgga taaatctaat aacaataaaa ttgatgacaa aacattcaat
     4081 aattataaag catctgaaaa taatgaaatt ctaaatcaat ttaaaaatga aagtgaaaat
     4141 ataaggaatc aagaggttgt tgaatttaca gaagaagatg aagaatatga agaagaactt
     4201 aaacaaaaca taattgaaaa tataaagaag aattttgatg aaaaaaaact agtacaggta
     4261 tataagtata ggggtgtata actttgaaaa aagaaggaat gtgactgtaa tagaattaat
     4321 agtatctatc agtataatac tcttgatagt tacattatct tttccaaaag ataatcttga
     4381 aaatcataaa ataaatttat ttgcacgtca actttgttca gatataaggt atgttaggcg
     4441 aattaatatg tttagcaact acaatacata tatatattat ataaatcaag gtaaacacga
     4501 aggttatgta ttgagaaaag atggtaaaaa cattaaatca ataatgctac ctaaaaatgc
     4561 aaaaatagct agaagtattg aaataataaa gtttaaatct gatgggtcgc ctcaaaaggc
     4621 gtctacaata gagatatata ataatagatt aagaaaaact ataacaataa cacctgtaag
     4681 tggaagagta cttctgaaag agggaaaata tgaaacataa atatggttac ttgcttttgg
     4741 aaagtgtagt atcattgtct tcaatggtga taattatact agttttatat tctatatttt
     4801 tgtctacaat aaacttaaag ttaaaagtag aagataagat agaactacaa caacaaagtc
     4861 ttgaaatcat aaaatctatg gaaggaatta ttagcaattc tatgggaata atgaatgtat
     4921 ctaattatga agaaacattt aaaaagacaa cgtctataaa atgtagatat gtagatgaaa
     4981 ataataatga agaaagcata agtaataaag aaattatatt gaatgaaaga cgaaataagt
     5041 tatttgtaaa ttctttaaat ggcgaaagta gtcaagcagg tggttatgaa attggagatt
     5101 atgtagatga gatgtatgta ttaattacta ataatggtca atatgttaat attaaattga
     5161 aattatcaaa acgtagtcaa aaatatgaga ctgatttcaa aataaaagta tggaatttta
     5221 gtgaaagtat ataaaaatac ataaatttaa gataaagaaa tgacatcaat aagtcatata
     5281 aaatataatt attgagaact atataactaa ataatatcaa tatgtaaagt gtatagatta
     5341 agaatataga ggataataat aaaaatatag aataagtaat ttgccaaata cttgtttata
     5401 ttgtataact tatgatatta tgatattagt aaaaatttgt aatcctgact tgacagaatt
     5461 caagtaagag atataatttt aaaggcatag taatgtaaaa tttactaaaa aataagaaat
     5521 ttattttaat atggaggaac aaaaaatggt atcagcaggt gattttagaa aaggtgttac
     5581 atttgaaaaa gatggacaac catgtttagt agttgatttt caacacgtta agccaggtaa
     5641 aggagctgct ttcgttagaa ctaaatacaa aaacttaaaa acaggagcta taagagaaga
     5701 aagtttcaat ccaagtgaaa aattccctaa ggctgttata gatacaagac aaatgcaata
     5761 tctatacaat gatggtgagt tatattattt tatggatcaa gaaaattttg aacaaatacc
     5821 attaaactat gagcaagttg aagatgcaat taagttctta aaagaaaatg aagttgctac
     5881 aataagattt taccaaggac aaccattcca agtggaagcg ccaaactttg cagagctaga
     5941 agttacagat acagagccag gtataaaagg tgatacagca agtaatgtaa ctaaagcagc
     6001 tacagttgaa actggagcag ttgttcaagt tccattattt ataaatactg gagataaagt
     6061 aaagatagat actagaacag gtgaatattt atcaagagta taaaaattta tgtatataaa
     6121 agcttatatg tcttaataat ataagatata tgagctttta gtttatatat aaaaaagaaa
     6181 gcgaggggtg ttacatgaaa catttatatg aagaagttgc atacttaaaa ggcctagcag
     6241 aaggactaga aataagtgct gaaagtaaag aagggaaaat gatacataaa atagttgatg
     6301 cattagaagt atttgcagat gctattgtaa cattggatga agaacaagaa gaacttcaag
     6361 attttgttga atctatagat gaagatttag cagatttaga agaagatatt gctgacatgg
     6421 aagaagatct ttatgaagaa gatgacgatg aggatgatga ggacttcagc tatatagaaa
     6481 tggaatgccc aaattgtgga gagttagtag aaatagacga agatttgtta tatgatgatg
     6541 aagtagatgt tgtttgtcct gattgtaagg ctgtgatatt atcatcagaa gatgattgtg
     6601 atgatgattg tacatgtggt ggttgtccaa gttgtgatga tagagagtaa aaattcataa
     6661 gaattaaaac tgcctatgat gcctatgaga tattttgctt cataggtagt tttattttta
     6721 tgaggaaggg tattatgtat tttggttttg caatacttgg acaaaaatag tacactagaa
     6781 tttatagaat aggattttgg agggatgtaa atatgaaaat aagtaaaaaa ttactagata
     6841 atatacaaag atttgaaaat gttataaatt ataaatttaa aaataaagaa tatatactag
     6901 aagctcttac tcatagttca tattctaatg aaaataaaaa atataatttt aacgaaaggt
     6961 tggaattttt aggggattcg gttttaggca tagtgataag tgattattta tttaatgaag
     7021 aagctaatct gcccgaaggg gaactaacta aacttagagc aaatatagtt tgtgaagatt
     7081 cgttaagtga agtagctaat gatataaatt taggtattca tatgttatta ggtagaggag
     7141 aagaagcaac aggaggaaga cataggactt caatattagc agacgcattt gaagctgtaa
     7201 tagctgctat atatcttgat ggaggttttg aaagtgcaag acagtttata cttcatcata
     7261 tggaaaatat aatttatgat tcaagaaaag gaaatatatt tagagactac aaaacgcatc
     7321 ttcaagaagt gttacaaggg aatggagaaa ataatatctg gtacaggctt atagaagaaa
     7381 aaggaccaga ccataataaa agatttgtaa tggaagttgg aattaatgac gatgtattgg
     7441 gtataggaga aggaaagagt aaaaaagaag ctgaacagtt agctgctaaa atagctttaa
     7501 agaaaaaatt atgggaaaaa taaatataat atgtaaaaat ataaaaagaa atatttatat
     7561 tagaaggatg ttgtagatga aaaagagaat tatacctata tttgttcctc acaaaggatg
     7621 tcctcatgat tgcatatttt gtaatcaaaa aaaaataaca ggtgtgagta ctgatgttac
     7681 tagtgaagat gcaagaaata taattgagga atgtttagag actatagata aagatgcaga
     7741 tgtagaaata gcattttttg gtggtagctt tactgctata gatattgata tacaaaaaag
     7801 tttattatca gttgctaaag aatatgtaga aaaaggtctt gtaaaagata ttagaatgtc
     7861 tacaagacca gattgtattg atgaagatat attaagtatg ctaaaagaat ataagacaag
     7921 tataattgaa ctaggagtac aatccttaga tgaaaaagta ttattagata gtataagagg
     7981 tcatcaatct gaaattgtat acaagagttc aaagatgata aaaaatagtg gaatcaaact
     8041 tggattacag atgatgattg gtcttccagc tgacacagaa gaaaagtgta tatttacagc
     8101 aaaaaaattt gtagagctaa aaccagattg tgttagagtc tatccaactt tggtagtaaa
     8161 agatacaggt cttgaaaagt taatggaaca aaataaatat aatccattta ctcttgaaga
     8221 gagtgtaaat atagtaaaaa aagttcttgt actgttttat gttaataata tcaatgtaat
     8281 aagagttggg cttcaagcta ctgatgatat acaaattgga aaagctgttt tagcaggacc
     8341 atatcatcct gcatttagag agttggtaga agcagatatg ataaaagatt atttagaatt
     8401 tgttattttg caaaataaaa atataaagca aatgttggtc aaatctaata aaaagaatat
     8461 ttctaaaatc ataggaaata aaaaaacaaa tgttaaatat atggaagaaa aatttggtgt
     8521 gcttttaaaa acacaagaat ccgatttaga tataaatcaa ttagaaattg tgttagatgg
     8581 gaaaagttta attagtgcta atatgagaga cattcataga aaattgtatg acatttatga
     8641 cctttagttt taataatgaa gggagagaaa agatttgtat ttaaaaagac tagagttaaa
     8701 gggatttaaa tcctttccag taaaaacaga tattattttt aaagaaggaa taacagccat
     8761 agtaggacca aatgggagtg gaaaaagtaa tatatctgat gctgtaagat gggtattagg
     8821 agaacagagt attaagagtc ttagaggtga taaactcgaa gatgtaatat ttgcaggtac
     8881 agatacaaaa aaacctatga attattgtga agtggctctt actattgata attctgaaaa
     8941 tcaattggaa ttagatttta cagaagtaac tataagaaga agggcttata gaaatggtga
     9001 aagtgaattt tttctaaata ataaaagttg tagattaaaa gatataaaag aagtcttttt
     9061 agatactggt attggaaaag atggttattc aatcatagaa caaggaaaag tagatgaaat
     9121 attaagtaat aatcctttaa gcagaagaaa agtttttgat gaagcttgtg gcatatcaaa
     9181 atatagatat aaaaaacagg aagcagaaag gaatttaagt aatacaaagg aaaatttaga
     9241 aagaatagat gatgtatata tagaaattga aaatcaatta aagcctcttt ttaatcaaca
     9301 gacaaaggca aaaaagtact tggaaataag tgaaaaatta aagatacttg aagtaaatag
     9361 ctttataaga gaaattgaag gaatagaaaa agaattaagt gaggttaatg aacatcgtaa
     9421 ggttattgaa aaggagctca atgaaaaaga agaacaaaaa aatgttgtag agaaaaagca
     9481 agaagatatt aataaagagg tagaggtatt acaagatgta attgaaaaat ctgtagatta
     9541 tataaattca attaaaggag ttatatcaaa aaaagagtcc caaataaatt taattaagga
     9601 aagaataaga aattttacga atgaaattag tagaaaaaat ttagaaataa atgatataaa
     9661 agagaagcta aatgaaaata agcaatacat aaaagaatta gaaagtaata aattatcagg
     9721 tagtgaagaa ctatctacct tgcaggaaaa tataaaagta ttggaggcaa gtaaagataa
     9781 acaaaaaatt aagttagaat ccctaaataa tgagatagaa ttattgaaag aaagtataat
     9841 tgatatatta aataagaaac aagagtttag taataagctg tccactttaa acgcaaataa
     9901 agaaaatatg aatatcagag atgaaaatat aaattcagag ataacagagt taaacaaaaa
     9961 tattgaaata aaatcatctg aattagatac tataaataaa gaatttaata tgcaaaatga
    10021 aaatttaaag aatgtaaata atagacataa agaattatca attaatttgc aggattcaat
    10081 tagtgaacac aataaactag aagatgaaat acagaaaagt aaatataatt taaatggata
    10141 taattcaaag ttaaatgtct atatagatat ggaaaatcat tatgaaggat ttaatagagg
    10201 agtaaaagaa gtactaaaaa ataaaaattt aaaaggagtt catggagcat taggacaaat
    10261 tataaatgta cctgaaaaat atgagaaatc tatagaagca gctcttggtg cttatatgca
    10321 aaatataata actgataatg agtttagtgc taagtctgca ataaactatc taaaacagaa
    10381 taatttaggt agagtaacat ttcttccatt aaatataatt aaatcaaata aagtaagtct
    10441 aggaaattta aaagcaaata ctaagttcat aggaatagca agtgatttaa ttacatttga
    10501 tgaaaagtat agaaatataa ttgaaaatat acttggaaga acaatactta taaataatat
    10561 agatgagggc ataaaatttg caaaagaaac tggtcatagg tttaaaatag taactttaga
    10621 tggtgaaatc ttaaatccag gaggttcttt gactggggga agtctaaaaa ctaatgggaa
    10681 tatactatcg agaaaaagat acattaatga atatacagaa aagataagta atatcaagaa
    10741 tgaaatttca catctagaat taaaaagaga atctttagac aaagatgtga aaaatatcaa
    10801 aaatgaaatt gattcacatg aaagtaaaat aaaagattta gaaaaaagta taataataaa
    10861 aagtacaagt ataaagaatg ttgaatctga aattgaatcg ttaaaaggta gtattactaa
    10921 attagaaaat gagaagaatg atttaaattc taatctaaat tatacacttg aaaaaagcga
    10981 tgatgttaga aaagatatgg aagagttaga tgatttatac aataaaaata aagaaaaaat
    11041 tgatgcattg aatgaggaaa taaaaagata caatgattta tatgataaag aaaaatctga
    11101 atttgatgaa ttgaacttaa gtttagtgaa gaaaacagaa gcttataata gtatagttag
    11161 agatataaaa agaatatctg gtgaaaattg tgagctagaa gaaaagaata aacaattaga
    11221 agagtcatta aattatgaag aacatgaaat aattaaatta caagattcta tagccactga
    11281 agaaaaagaa aaagaaaatt taacaaaaca actaggggat agtaatagaa atcttgaaac
    11341 tagaaaaata gcaaaagatg atttaaaaaa tagttttgat gaaattaata aagaattaaa
    11401 aactatagat agacagcata tagagcttaa agaaagttta tttaaagttg gtggaagatt
    11461 agaaagatta aaaactagtc aagacacata tataaataag ttgtttgagc aatatgatat
    11521 gactttagta caagcattgg aaattaaaga tgaagatttg gatatagata gaaaattctt
    11581 agaaagtcta aagagagaaa taagaagtct tggaaatata aatataggct ctataaagga
    11641 atatgaggaa ataaaagaaa gatacgattt ttatagcgaa caaaagcaag atttagaaga
    11701 gtctatggaa gagatagaaa aacttataca cactttagaa gaaaatatga aatctgaatt
    11761 tgaaattaag tttgaagaaa taagtaaaaa ttttaaatat gtatataaga gattatttgg
    11821 tggaggttgt ggagaattaa ctattttaga taaagaaaat ttattagaaa gtgatatact
    11881 aataacagct caacctccag ggaaaaaaat gaaaaattta aatctattat ctggtggaga
    11941 gaaagcttta acagcaataa gtatattatt tgctatattg attacaaagc caacaccatt
    12001 ttgtatatta gatgagattg aagcgccact tgatgatgca aatatattta gatttggaga
    12061 atttttgaag gatttatcta aagagactca atttatatca gtaactcata ggagaggaac
    12121 tatggaagct gctgattata tctatggagt tacaatgcaa gaaaaagcca tttcaaaagt
    12181 tattagctta aaattaaaag aagctcagga aataacagat attatataac ttagattgac
    12241 ataggaggaa taacagtgtt aaaaaaatta tttggttttg gaaaagacaa ggaaaaagaa
    12301 atagaaaaaa aagatgctga agaagaaata gaagttgaag acagtgttga tagtttagaa
    12361 aacttggaag aaaccatttt ttcaggactt gaagaagaag tgatagataa agtggaagat
    12421 attgaagaaa aaactgagga aagtgtcaat aatgaaggca ctgaagaaat cgaaaatttt
    12481 gaaaaaatag aagttgagat ggatagtaat gcaaaagaag tagaaaatat ttcaaataat
    12541 gattctcaag aagagataat cgaagaatta gaaaattatg atgaaattga gtctaaagag
    12601 atagaagata aagaagataa gaaagttaac ttgtttgaaa gactaaaaca aggattgaca
    12661 aaagcaaagc aaggaataac tgatagaata gatgaagttt taaaatcata tacaaaaata
    12721 gatgaagaat tacttgaaga tttagaggaa attttaataa cagcagatgt tggtgtaaat
    12781 acaactatgg atataattga aagactaaga gataaaataa agcaaaaagg tattactgaa
    12841 ccaataaagg taagagaaga acttaaatct atagttgaag atattcttac aaatgaaaac
    12901 tctacactag atatagaacc agcaccatgt ataatattaa tggtgggtgt aaatggggtc
    12961 ggtaagacta ctacaatagg taaactagca aatagataca aaaaagatgg taaaaaagtt
    13021 ttattagcag cagcagatac atttagagca gcagcaacag agcaattaga aatttgggca
    13081 aatagaacca atgtagacat aataaaacat caagagggag cagaccctgg agctgtagta
    13141 tttgatgcta taaaagctgc aaaagctaga aaaacagatg tattaatatg tgatacagca
    13201 ggaagattac acaataaggc taatcttatg aatgaacttg gaaaagtatt taaaatagta
    13261 gatagagaat ttccagaagc aaaacgtgaa gtacttcttg tggtggatgc tacaacaggt
    13321 caaaatgcag ttgttcaagc aaagacattt aaagaagttg cagatataac aggtatagta
    13381 cttacaaaac ttgatggaac tgcaaaaggt ggagtagtcc ttgcagtaaa atcagaagta
    13441 gatgtacctg taaaacttat tggagttgga gaaagtgtag aagacctaca agatttcaat
    13501 gcaaaatcat tttcagatgc attatttggt aactaatgtt cttttaaaaa taatggttga
    13561 caagagtgtt gttatgatat aaaatacaat agtgtaaagt aaataacctt tacagtatac
    13621 tgctaggaag tgtaagatat gaatatagaa aaaatggtcg aaattggatt gttatttgaa
    13681 cagtataaag aattattaac tgacaagcag aaagaaatag ttgctctata ctatgaagaa
    13741 gattattctt taggtgaaat tagtgaaaat ctgaatgtat caagacaagg tgtttatgat
    13801 accttaaagc gttcagaaaa aatactaaga gattatgaag aaaagttaca cttagtatca
    13861 aagattcaag agcaggaaaa aaatattaag attataaaag ataaaattat tgacattaaa
    13921 gaagatttat tgcacaatag agattgtgct aatttaatcc ctaagctaga aaatatagaa
    13981 gatgtatgta gggagatgat aaaatgatat ttgaaggatt gtctgataaa ctacaagggg
    14041 catttagtaa gttaaaatct aaaggaaaac ttacagaagt agatgtaaaa aatgctatga
    14101 gagaagttaa acttgctctt cttgaggcag atgttaactt taaagtagtt aaagatttca
    14161 taaaaaaagt tcaagagaga tgtgttggac aagaagttat gcaaagctta actccagctc
    14221 agcatgtaat caaaattgta aatgaagagc ttacaagttt aatgggagat gtacaaagta
    14281 aagtaatgat ttcctcaaag cctcctacaa ttttaatgat ggttggattg caaggtgcag
    14341 gtaagactac tacagcagga aaacttggag gatattttaa aaagcaaggt aaaaaacctc
    14401 tattagtagc ttgtgatatt tatagaccgg cagctataaa gcaacttcaa gttgttggag
    14461 aaaaattgga tatacctgta ttcaatatgg gagataaaga aaatccagta aatattgcga
    14521 aagcaggttt aagccatgcg attaaaaatg ctaatgattt agttattata gatacagctg
    14581 gtagacttca tatagatgaa gtacttatgg ctgagttaaa gtctattaaa tcagaagtta
    14641 agccacatga gatacttttg gttgtagatt ctatgacagg tcaagatgct gtaaatgttg
    14701 cagaaagctt caatgaggca cttggtgttg atggagtagt attaactaaa ttggatggag
    14761 atactagagg tggtgctgca ctttcaataa gagcagttac acaaaagcct ataaaattta
    14821 tggggatggg agaaaagcta gatgatatag aacctttcca cccagataga atggcatcaa
    14881 gaatattagg tatgggagat gtcttaagtt taatagaaaa agcacaagaa aatatagact
    14941 tagagaaagc taaagaatta gagcaaaaaa ttaaaaaaca agagcttgat tttgaagatt
    15001 tcttacaaca aatggaacaa atacaaaata tgggacctct tgacaagata cttggtatga
    15061 tacctggtat gggaaatgta aaagaccaac ttggagatgt agatttgaat ggcaaagaaa
    15121 tgaagagaac aaaagccata attcagtcaa tgactacata tgagagaaga aacccaggag
    15181 tattaaatgc ttcaagaaag aaaagaatag ctaagggcag tggaactagt gtacaagatg
    15241 taaatagact tataaaacaa cttaatgaaa tgaaaaaaat gatgaagatg tttgctggtt
    15301 cacaaaagag tatgaagaaa aggggcggct taggcggttt accttttttt aaataaaata
    15361 taaaaaagta aaaattattc ggaggtgact gtaatggcag ttaaaataag attaaaaaga
    15421 atgggagcta acaaaaaacc tttctacaga atagtagtag ctgattcaag agctccaaga
    15481 gatggaaaat ttatagaaga aataggatac tataatccaa tttctgaacc taaacaagta
    15541 agaataaatg atgaaaaagc tataaaatgg ttagctactg gtgctcagcc aacagaagta
    15601 gttaaaaaat tacttgtaaa aaacggagta atagaaaaat tcgaagcttc taaacaagca
    15661 aaataatttt ttcttggcaa ttagtttaag taagggctca ttgctaaggt tactttagga
    15721 gatgaaatat atgaaagagc tagttgtaga tatagctaag gctctagttg ataatcctga
    15781 ctcagttgtt gttgaggaat ttgaagataa tgatggtatc gtcttaaagc ttacagttgc
    15841 tcaagaggat atgggtaagg ttattgggaa gcagggaaga atagctaaag ctataagaac
    15901 tgtcgtaaga tctgttgcaa atagagaaaa tataaaagtt tctcttgaga tagtataaaa
    15961 taggaggatt aggtataatg cctagtcctt tttatataaa tgcgtagttt aagattttat
    16021 gatgatatga ccacataaga ttaaattttt taagaaggtg ataacatgaa agaaaaatta
    16081 actcatttta aggtaggtca aatagtaaat acacaaggtt taaaaggtga agtaagagtt
    16141 tatccactta ctgatgatat agagaggttt gatgaattaa aagatttcta tctgggaaaa
    16201 gatttaaata ataaattggc tgttgaaaaa gtaagataca aaggttctat ggttataatg
    16261 aagataaaag gcatagacag catagagaaa gcagaaaaac taaaaaataa atttatgtat
    16321 gtatctagag aagatagcag agagcttgaa gaaggcgaat tttttatagc agatatgatt
    16381 ggaatggaag ttttaactgt agatggaaaa tatgtaggaa tactagaaga tgttcttcaa
    16441 tactcagcaa atgatgttta tgtagtaaaa ggtgaagaag ataaagaatt tatgatacca
    16501 gctataaaaa aatttgtacc tacaatagat attgatgaaa gaaaaatgat tatagaccct
    16561 attaagggaa tgatagatta ggtggtaata tgagatttca tataatgaca ctttttccag
    16621 aaatatttaa ttcgtacatg gatgaaagta taatgaagag agcagttgaa aaaggtataa
    16681 ttgaggttta tatatataat ataagggatt tttctaataa taaacataaa aaagtagatg
    16741 attatccatt tggaggagga gtggggatgg taatgactcc tcaacctata tatgacacat
    16801 ataagcatat aataacaact cacaatataa gtaatccaag tgtaatttat cttactccaa
    16861 aaggtaaggt atacaatcaa tctatagcaa agcagatgtc tttaaaagaa gatataatac
    16921 tattatgtgg ccattatgaa ggagtagatg agagaatcat tgatttaata gttactgatg
    16981 aaatttcaat tggagattat gtattaactg ggggagagct cccagcactt ataatgatag
    17041 attctatatc aagattaata ccaggtgtat taaaccaaga agaatcattt gaagaagaat
    17101 catttaaaga taacttattg gaatatcctc attacactag acctagagat tttgaaggat
    17161 taaaagtacc agaagtgctt ttatcaggga atcataaaaa gatagatgag tggagaagag
    17221 aagaatctat aaggataacc aaagaaagac gatttgattt atataaaaag tctaatgaaa
    17281 aataaaaata taatgaaaaa ttaatatata tgtatacaat aaagtaccta taaatggaat
    17341 taaaaacata aaagtgcttg taataactat tgaagtatag tataatataa aatgttgaaa
    17401 atacgggcgt tcctctgttt acactggtcg ggtagttaag aacgtctatg atatctagga
    17461 ggaagaaaga tgaatgaaat gctaagagca attgagcaag aacaattaaa aaatgaagtt
    17521 cctaattttg gacctgggga cacagttaaa gtacacgtta gaattataga aggaaaaaga
    17581 gaaagaatac aagtattcga aggtgtagta ttaaagagac aaggtggagg agcaagagaa
    17641 actttcacag ttagaaaaat gtctttcaat gttggtgtag aaagaacttt cccagttcac
    17701 tctccaaaaa tagaaaaaat agaagttact agaaaaggta aagtaagaag agctaaacta
    17761 aactacttaa gaggtagagt aggtaaagct gctaagataa aagaagctag aaataaataa
    17821 ttaaaaactt tacttataaa tggggggctg atttatcagt cctctttcta ttttaatgaa
    17881 aattacagca aataattata ctagaaataa atagaaatat ttgttgaaaa tatacattat
    17941 ttatctatga atttatagct tttaaaatga aaaataaaat tggtaaatat tggattatat
    18001 tttgtaacga tattaaaaat aagagaacag tattatgtaa agtatgacgt taaaaagatg
    18061 acaatattat agtttgtgaa gatagatttt ttaagtggaa ttagaatata ataatataat
    18121 attaataagg agtgatacat gtgtcaaatg aatacgaaga gtatcttatg gatgacaatc
    18181 tacatataaa ctggtatcca ggtcatatga aaaaaactaa ggagttagta aaaaataact
    18241 taaaattgat tgatgtagta attgaacttt tggatgctag aatcccgttt agtagtaaaa
    18301 atccagacat agacaggctt gtaggagata agccaagagt tgtcgtactt aataagagtg
    18361 atatggcaga tagggataaa ttgaatcaat ggattgaata ctataagaaa attaatatca
    18421 aagctatacc agtagataca ataaaaggtg ttggtataaa taatataatc gaagaatgta
    18481 aaaatgtaac tagagaaaaa atgagttctt taaaagacaa agggagaaaa gaaagagcta
    18541 tacgaataat gatagttgga gttccaaacg ttggtaaatc gtctcttata aacaaattaa
    18601 caggtagaaa aagcactcaa actggagata agccaggagt aacgaaaggg aaacaatggg
    18661 ttagattaaa gggaaatctt gaacttttag atacacctgg aatactttgg cctaagtttg
    18721 aagaccaaga agttgcatta aatttagcat ttagtagagc tataaaggat gagattttag
    18781 atactgagac tttggcttta agacttatag aaaaacttat gaaaatagaa cctgagaagt
    18841 tgaaagccag atataagtta gattgtctag gagaaactcc aattgaaact atggatatga
    18901 ttggccataa aagagggttt ataacaggta aaaaagaact agattataca cgcatagccg
    18961 caactgtatt aaatgaattt agagatggaa aaataggaaa tataacattg gaagttccag
    19021 aaaacgttaa aagatagatt ttaaaacaaa atcaaagatt taatgttaaa aaaataaaaa
    19081 agccttttag caaaggcttt tttatttttt tgaaatatag acaaatatct ccatataaaa
    19141 gtataaatta ggttagaaaa cctttaaaaa tattaattta agacaaatac acctaatatt
    19201 ttgaaaatta aaatgagtgt gattatttta aattttagca tattgacttg cttgtttcgt
    19261 ataatatata ataagcacaa ataaatacaa tatattaaaa atttaattta tttaatttta
    19321 ttaatttaat aataagttct tattgtttaa tttaatttat ttatgcaagc gtaaacaatc
    19381 gagacatttt attaatttaa taaggttaaa aagggggatt ttataatggg taagaccaag
    19441 gatgttatag tatttggatt tgcgttattt gcaatgttct ttggagcagg taacttaata
    19501 tttccacctt acttgggaat aattacaggt ccagaatggt taattgcctt cttaggattt
    19561 acgtttgctg atgctggatt ggcacttcta gcagtaatgg ctacagctaa attcgatgga
    19621 aacgtagttg aaatgtttaa aagatgtggt atgaagttag ggattcttat aggttgtgca
    19681 gacattttat gtataggtcc attcttagct ataccaagaa caggtgcaac aacttatgaa
    19741 atgggtataa tgcctttatt tggaacttca attccagtgt tattattctg tatacttttt
    19801 tttgcgcttt catatgtact aacaataaga ccttctaagg tcgtagatat agttggacag
    19861 tttttaactc cagctctttt aatagctcta gcatttataa ttataaaagg tattatatca
    19921 cctcttggag atatagtaga taaacctatg atacctaatg tatttgcaga aggtataggt
    19981 caaggatatc agacaatgga tgcttttgca gcaatagcac ttgcatcagt gttaatagta
    20041 tctttaaatg ataaaggata ttctactatt agtgacaagt taaaaatgat tggtaaggcg
    20101 ggggttcttg cttgtggagg attagctcta gtttatggag gattatgttt ccttggagca
    20161 actgtatcaa caatgtatgg aacagatgca gtacaatcac aagtaatagt aaatataact
    20221 gaaggattac ttggaaatgt tgggaaagcg attttagcag tagtagtatc attagcatgt
    20281 ttaactacat ctataggtct tacatctgcc acaggtcaat atttctctag acttacaaaa
    20341 ggtaaattga gttatgaaaa aatagtatta gctgtatctg tattcagtgc agtagtagca
    20401 tcttttggtg ttggaactat aataaagata gcatctccta tactaagtat agtttatcca
    20461 ccatcaattg ttttaataat tttagctttc ttcaatgaaa aaattaagaa tgataatgtg
    20521 tacaaaggag cagtatatat gtcattgctt gtaagtatac taacagttat atctagttat
    20581 ggagtagatg ttccagtagt aaaatcttta cctcttcatt cattaggatt taactgggtg
    20641 gtaccagtta taatagctgg aataattggt aattttatac catcaaagag tcaatctaat
    20701 acacttggaa caaactaagt tttgtttaat taaaatataa tcaattgtaa ataaatgagt
    20761 taactaaaag taaaaggagt tatttgtaca aataactcct tttattt
//
LOCUS       DAMJVX010000058        20760 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMJVX010000058 DAMJVX010000000
VERSION     DAMJVX010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.060"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            358..669
                     /gene="rpsJ"
                     /locus_tag="PC407_001863"
     CDS             358..669
                     /gene="rpsJ"
                     /locus_tag="PC407_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDF2497309.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            761..1390
                     /gene="rplC"
                     /locus_tag="PC407_001864"
     CDS             761..1390
                     /gene="rplC"
                     /locus_tag="PC407_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDF2497310.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1420..2043
                     /gene="rplD"
                     /locus_tag="PC407_001865"
     CDS             1420..2043
                     /gene="rplD"
                     /locus_tag="PC407_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDF2497311.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2043..2333
                     /gene="rplW"
                     /locus_tag="PC407_001866"
     CDS             2043..2333
                     /gene="rplW"
                     /locus_tag="PC407_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDF2497312.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTASFKKAVVKLTADSKEIEFFQGM"
     gene            2363..3193
                     /gene="rplB"
                     /locus_tag="PC407_001867"
     CDS             2363..3193
                     /gene="rplB"
                     /locus_tag="PC407_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDF2497313.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3228..3509
                     /gene="rpsS"
                     /locus_tag="PC407_001868"
     CDS             3228..3509
                     /gene="rpsS"
                     /locus_tag="PC407_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDF2497314.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3541..3876
                     /gene="rplV"
                     /locus_tag="PC407_001869"
     CDS             3541..3876
                     /gene="rplV"
                     /locus_tag="PC407_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDF2497315.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3899..4714
                     /gene="rpsC"
                     /locus_tag="PC407_001870"
     CDS             3899..4714
                     /gene="rpsC"
                     /locus_tag="PC407_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDF2497316.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4750..5181
                     /gene="rplP"
                     /locus_tag="PC407_001871"
     CDS             4750..5181
                     /gene="rplP"
                     /locus_tag="PC407_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDF2497317.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5183..5386
                     /gene="rpmC"
                     /locus_tag="PC407_001872"
     CDS             5183..5386
                     /gene="rpmC"
                     /locus_tag="PC407_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDF2497318.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5410..5664
                     /gene="rpsQ"
                     /locus_tag="PC407_001873"
     CDS             5410..5664
                     /gene="rpsQ"
                     /locus_tag="PC407_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDF2497319.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5690..6058
                     /gene="rplN"
                     /locus_tag="PC407_001874"
     CDS             5690..6058
                     /gene="rplN"
                     /locus_tag="PC407_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDF2497320.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6082..6387
                     /gene="rplX"
                     /locus_tag="PC407_001875"
     CDS             6082..6387
                     /gene="rplX"
                     /locus_tag="PC407_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDF2497321.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6417..6959
                     /gene="rplE"
                     /locus_tag="PC407_001876"
     CDS             6417..6959
                     /gene="rplE"
                     /locus_tag="PC407_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDF2497322.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6977..7162
                     /locus_tag="PC407_001877"
     CDS             6977..7162
                     /locus_tag="PC407_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDF2497323.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7195..7593
                     /gene="rpsH"
                     /locus_tag="PC407_001878"
     CDS             7195..7593
                     /gene="rpsH"
                     /locus_tag="PC407_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDF2497324.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7624..8166
                     /gene="rplF"
                     /locus_tag="PC407_001879"
     CDS             7624..8166
                     /gene="rplF"
                     /locus_tag="PC407_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDF2497325.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8202..8570
                     /gene="rplR"
                     /locus_tag="PC407_001880"
     CDS             8202..8570
                     /gene="rplR"
                     /locus_tag="PC407_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDF2497326.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8591..9100
                     /gene="rpsE"
                     /locus_tag="PC407_001881"
     CDS             8591..9100
                     /gene="rpsE"
                     /locus_tag="PC407_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDF2497327.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9115..9300
                     /gene="rpmD"
                     /locus_tag="PC407_001882"
     CDS             9115..9300
                     /gene="rpmD"
                     /locus_tag="PC407_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDF2497328.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9333..9776
                     /gene="rplO"
                     /locus_tag="PC407_001883"
     CDS             9333..9776
                     /gene="rplO"
                     /locus_tag="PC407_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDF2497329.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9820..11088
                     /gene="secY"
                     /locus_tag="PC407_001884"
     CDS             9820..11088
                     /gene="secY"
                     /locus_tag="PC407_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDF2497330.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11105..11755
                     /locus_tag="PC407_001885"
     CDS             11105..11755
                     /locus_tag="PC407_001885"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDF2497331.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11756..12502
                     /gene="map"
                     /locus_tag="PC407_001886"
     CDS             11756..12502
                     /gene="map"
                     /locus_tag="PC407_001886"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDF2497332.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12524..12793
                     /locus_tag="PC407_001887"
     CDS             12524..12793
                     /locus_tag="PC407_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HDF2497333.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYDFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12831..13049
                     /gene="infA"
                     /locus_tag="PC407_001888"
     CDS             12831..13049
                     /gene="infA"
                     /locus_tag="PC407_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDF2497334.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13085..13198
                     /gene="rpmJ"
                     /locus_tag="PC407_001889"
     CDS             13085..13198
                     /gene="rpmJ"
                     /locus_tag="PC407_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDF2497335.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13351..13722
                     /gene="rpsM"
                     /locus_tag="PC407_001890"
     CDS             13351..13722
                     /gene="rpsM"
                     /locus_tag="PC407_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDF2497336.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13758..14156
                     /gene="rpsK"
                     /locus_tag="PC407_001891"
     CDS             13758..14156
                     /gene="rpsK"
                     /locus_tag="PC407_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDF2497337.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14188..14811
                     /gene="rpsD"
                     /locus_tag="PC407_001892"
     CDS             14188..14811
                     /gene="rpsD"
                     /locus_tag="PC407_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDF2497338.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14889..15836
                     /locus_tag="PC407_001893"
     CDS             14889..15836
                     /locus_tag="PC407_001893"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDF2497339.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15857..16198
                     /gene="rplQ"
                     /locus_tag="PC407_001894"
     CDS             15857..16198
                     /gene="rplQ"
                     /locus_tag="PC407_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDF2497340.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16318..17151
                     /locus_tag="PC407_001895"
     CDS             16318..17151
                     /locus_tag="PC407_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2497341.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17139..18005
                     /locus_tag="PC407_001896"
     CDS             17139..18005
                     /locus_tag="PC407_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2497342.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDISFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            17999..18802
                     /locus_tag="PC407_001897"
     CDS             17999..18802
                     /locus_tag="PC407_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDF2497343.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18834..19565
                     /gene="truA"
                     /locus_tag="PC407_001898"
     CDS             18834..19565
                     /gene="truA"
                     /locus_tag="PC407_001898"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2497344.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19685..20116
                     /gene="rplM"
                     /locus_tag="PC407_001899"
     CDS             19685..20116
                     /gene="rplM"
                     /locus_tag="PC407_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDF2497345.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20145..20537
                     /gene="rpsI"
                     /locus_tag="PC407_001900"
     CDS             20145..20537
                     /gene="rpsI"
                     /locus_tag="PC407_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDF2497346.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8289 a         2445 c         4327 g         5699 t
ORIGIN      
        1 atggatttaa tataaaaaaa agaggggggt aactcctctc ttttttgttt tttgttgaca
       61 agtagcgcta aagcatatat actaaactag tgccttgaga cagattaaca atcaagttta
      121 aggttatgat gttgtaatag aaaataaaat tgaaaaaaac agaaaaaaaa cttgaaaaaa
      181 tgtaacagat gatataaaat ataatagtgt gcttgagaga ttaagaaaat aataattaaa
      241 tcctagtgaa atacgaactg atatcgattt aaaagttaca acacacccgg agcagtgtat
      301 cggtattggt tgtacgcaac ttgaaaatta aacacgtgat aaaggaggga aaacaaaatg
      361 gctaagaatg aaaaaataag aataagatta aaatcatatg atcacaaatt attagatttt
      421 tcagcaggta aaatagttga aactgctaaa aaggctggat cacaagtttc aggacctgtg
      481 cctctaccaa cagaaaaaca agttgtaact atattaagag ctgtacataa gtacaaagac
      541 tctagagaac aatttgaaat aagaactcat aaaagactaa tcgacatagc taatccaaca
      601 cctaagacag ttgactcatt aatgagatta gacttaccag caggtgtaga tatagaaata
      661 aaattataag aacccctaag atattatcct atagatgatt atcttagaat gattactcta
      721 aaagatgtaa tccgctgtaa gaattatagg aggtgctaat atgaaaggaa tattaggcaa
      781 aaaagttggt atgactcaaa tattcactga taaaggtgta gttatacctg taacagctgt
      841 tgaagcaggg cctatggtcg ttactcaaat aaaaacagtt gataaagatg gatacaatgc
      901 tatacaaata ggttttgaag atgctaaaga aaaagcttta aacaaaccta aaaaaggaca
      961 tttagctgct gctaatgttt taaagaaaca tttaaaagaa tttagagtag attctgtaga
     1021 aggatacaca gtaggacaag aaataaaagc tgatgtattt gaagcaggtg caaaaataga
     1081 tgttactgga ataagtaaag gtaagggatt ccaaggtcca ataaaaagac atggacaaag
     1141 tagaggtcct gaaactcatg gttctagata ccacagaaga ccaggttcaa tgggagcttg
     1201 ttcttaccca ggtagagtat ttaaaaacaa aaaattagct ggacatatgg gaagcgtaaa
     1261 ggtaacagtt caaaacttag aggttgttaa agtagatgct gacaagaacc ttatattagt
     1321 taaaggagct ataccaggag ctaaaggttc agtagtaact ataaaagaag ctataaaggt
     1381 ttctaaataa tgactaacct agagaaagga ggaataacaa tgccaaaatt aaatgtacta
     1441 aatgttagtg gacaaaacgt tggagaaata gaattatcag attctatatt tggcgtagaa
     1501 gttaatggac atgttttata cgaagttgta aaaaatcaat tagcaaataa gagacaagga
     1561 actcaatctg ctaaaactag agcagaagtt agaggtggcg gaagaaaacc ttggaaacaa
     1621 aaaggaacag gtagagcaag acaaggttct acaagatctg tacaatgggt aggtggagga
     1681 gttgcttttg cacctaaacc aagaagctac aaatatacat tacctaagaa ggtaagaaga
     1741 ttagctatga agagtgcttt atcttcaaaa gttcaaaata gtgaagttat agtattagat
     1801 gcattaaaca tggatgctcc taagactaaa gaatttgctc aaatattaaa caatataaat
     1861 gctgctaaaa aagctttagt agttatagct gacaaaaacg ataacgtaat aaaatcagct
     1921 agaaatatag aaggtgttca aactgcatta gttaatacta tgaatgtata tgatatatta
     1981 aaatacgatt catttataat aactacagat gctgttaaaa aagtggagga ggtgtacgca
     2041 taatgactaa tccacatgat gtaataataa gaccagttgt aactgagcac agtatggctg
     2101 aaatgggtga aaaaaaatac acttttgttg tggctaaaga tgcgaacaaa actgaaataa
     2161 aaaaagcagt agaaaaagta ttcggagtta gtgttgataa agtaaatact ttaaactacg
     2221 atggaaaagt taaaagaatg ggtagaactc aaggaagaac agcaagcttt aagaaagcag
     2281 tagttaaatt aactgctgat agtaaagaaa tagaattctt ccaaggaatg tagtattata
     2341 acaaacgaag gagggaaaac agatggctat aaaaaagttt agaccaactt ctcctgcctt
     2401 aagacaaatg acagttttag tttctgatga aataacatgt aatcaaccag aaaaatctct
     2461 tttagttaac ttaaagaaaa atgctggtag aaacgtacat ggtagaataa ctgttcgtca
     2521 tagaggtgga ggacaaaaaa gaaaatatag aataatagat tttaaaagag ataaagatgg
     2581 tatacctgct aaggttgcta ctatagaata tgatccaaac agaactgcaa atatcgcatt
     2641 attaaactat gctgatggag aaaaaagata tatattagct ccagttggaa ttaacgttgg
     2701 agatactata ttatcaggat taggtgctga tataaaacca ggaaactgtt tagcattaaa
     2761 agatatgcca gttggtacta tcattcataa tatagaatta aagccaggaa aaggagctca
     2821 attagttaga tctgctggag tttctgcaca attaatggct aaagaaggaa aaaatgcttt
     2881 attaagatta ccatcaggtg agatgagatt agtaagcata aactgtaaag ctactatagg
     2941 tcaagttgga aatatagagc atggtaacgt agttatcggt aaagcaggta gaaaaagaca
     3001 tatgggaata agacctactg taagaggttc tgtaatgaac cctaatgacc atccacacgg
     3061 tggaggggaa ggtagatctc caataggtag accatctcct gttactccat ggggtaaacc
     3121 agctcttgga tacaaaacta gaaagaaaaa taaagcttca aataaactaa tagtatcaag
     3181 aagaactaag tagtaaattt atttttgctc ggaaaggagg aatataaatg tcaagatcaa
     3241 ctaaaaaagg gccttttgtc catgcaagac ttttaaagaa gatagaagct atgaatgcta
     3301 gtggaaataa agaagttata aaaacttggt caagatcttc aactgtattt ccacaaatgg
     3361 tagaaaacac tatagctgtt catgatggaa gaaaacatgt tccagtatat ataacagaag
     3421 atatggttgg acataaatta ggtgagttcg ttcctactag aactttcaaa ggacataagg
     3481 atgacgaaaa atctaataag agaaaataat taaattatga ctaagaaagg aggaataaca
     3541 atggaagcaa aagctactgc taaatacgta cgtgtatcac caagaaaagc aggccaaata
     3601 tgtgaccttg ttagaggaaa gaatgttgat gaagcattag caatattaaa gtttactcca
     3661 agaggagcag cttcaataat agctaaggtt gtaaaatcag ctaaagctaa cgcagaaaac
     3721 aatcacgaaa tggatactga aaaattatat atagcatcaa tagttgctaa tcaaggacca
     3781 acaatgaaga gattcatgcc tagagctatg ggtcgtgcaa ctacaataag aaaaagaact
     3841 tctcatatag aggttgttgt taaagaaaaa aaataattaa tagataggag ggaaagtaat
     3901 gggtcaaaag gttaatccac acggcttaag ggtcggtgtt ataaaagatt gggactcaag
     3961 atggttcgct actgataaaa aagagttcgg aaacttatta ttagaagacc ataatatacg
     4021 taaattctta aagaaaagat tatactcagc tggagttgct aagatagaaa tagaaagatc
     4081 agctaacaaa ataaaaatgg acttacacgt tgctaagcct ggtgtagtta taggaagagc
     4141 tggtgcagga atcgaagcat taaaagctga attagaaaaa atgacaaaga aaactataat
     4201 agttaatata gtagaagtaa gaagtacaga taaaaatgct caattagtag ctgaaaatat
     4261 agctttagct atagagagaa gggttgcttt cagaagagca atgaaacaag ctatacaaag
     4321 agcaatgaaa tctggtgcta aagggataaa agtttctgct tcaggaagat taggtggagc
     4381 tgaaatggct agaactgaag gttatagtga aggaaatgtg ccactacaaa ctttaagagc
     4441 agatatagat tatggtttcg ctgaagctga tacaacttat ggaaaaatcg gtataaaagt
     4501 ttggatatgt aatggtgaag ttttaccaac tagagacggt gtaaatccaa gagaagaaag
     4561 cagaaagagc gatagaagag acaacaagag agataataga agaaacgata gaagaggaaa
     4621 tgacagaaga ggaaatgata acagaggaaa ctacagagga caaagacctc aaggtggatc
     4681 aagacctcaa agaactgaaa acaaaggaaa ttaataatag ttaggttcaa ggaaggagga
     4741 aaaatactca tgttaatgcc aaaaagagta aagcgtcgta gagttcatag aggaagcatg
     4801 gctgggcaag ctcataaggg taataaagtt acttatggtg agtttggatt agtagcatta
     4861 gaagcttctt ggataacttc taatcaaata gaagctgcca gaatcgcgat gactagatat
     4921 ataaaaagag ggggaaaggt ttggataaaa atattccctc ataaaccagt aacaagaaaa
     4981 ccagcagaga ctcgtatggg tgcagggaaa ggttctccag aatattgggt agctgtagtt
     5041 aagccaggaa gagttatgtt tgaattagca ggtgtttctg aagataaagc aagagaagct
     5101 atgagacttg ctgcacataa attaccaatc aaatgtaaat ttgttaaaaa agaagattta
     5161 gaagtaaagg gtggtgaata ggatgaaagc taaagaatta agagatttaa caagcgaaga
     5221 gctaatgaac aaattaaacg acttcaaaag tgaattattt agcttaagat tccaattagc
     5281 tactggtcaa ttagaaaata cagctagaat aaagtttgtt aagaaggata tagcaaaagt
     5341 taaaactgtt cttgctgaaa gaaagttata cgaaactaga gcttaatttg gaaaggaggc
     5401 tttttaaaca tggaaagagg aagaagaaaa gttagaatag gccgtgttgt tagtgataag
     5461 atggataaaa caatagtagt agctgttgaa gaattcgtac gtcatccact atataataaa
     5521 cgtgttaaga gaactaagaa attcaaggct catgatgaaa agaatatatg taacatcgga
     5581 gatagagtta aaataatgga aactagacca ttatctaaag ataagagatt cagactagtt
     5641 gaagttgttg agaaagttaa gtagtttttg aagaaggagg gattacgata tgatacaaca
     5701 agaatcacgt ctaagagttg ctgataactc aggagctaaa gaacttttat gtatacgtgt
     5761 tctaggcgga agtaaaagaa gatatggtaa cataggcgac gttatagttg ctactgttaa
     5821 aagtgcaaca cctggtggag ttgtaaaaaa aggtaaggtt gttaaagctg ttatagtaag
     5881 aagcaaacaa ggcgtaagac gtaatgacgg aagctatata tcttttgatg aaaatgctgc
     5941 tgttataata aaggacgata aaactccagt aggaactcgt atattcgggc ctgttgctag
     6001 agagttaaga gacaatgaat tcatgaaaat agtttctctt gctccagaag tactataatt
     6061 aaggaggtgc aataggacat gatgcgtgtt aaaaaaggtg acactgttgt agttatagca
     6121 ggtaaagata aaggtaaaaa aggttcagtt ttaaaggtat atcctaaaac tagcaaagtg
     6181 ttagttgaag gtgtaaatgt aataactaaa catcaaaaac caagtgctat gaaccaacaa
     6241 ggtggaataa taaataaaga agccccaata cacatatcta atgtaatgcc atttgatcct
     6301 gaaacaggaa aaggcgttag agtaagatat gaagtaaaag atgggaacaa agtaagagta
     6361 tcagcaaaga gcggaaaaga attataataa atattgcttg aaaggaggga ccttaaatgg
     6421 cttctagatt acaagaaaaa tacatgaaag aagttgctcc tgctttaatg gagaaatttg
     6481 gatacaaaaa cgtaatggag atacctaagt taaataaaat agttattaac atgggtatag
     6541 gtgacgctag agaaaatcca aaaggattag aaaaaggcgt tgaagaatta gaaatgatat
     6601 caggacaaaa gcctgttata actaaagcta gaaaatctgt tgctaacttc aaattaagag
     6661 aaggaatgcc aataggaaca aaagttacat taagagctga caaaatgttc tactttatgg
     6721 acaaattagt ttcagtttct ttaccaagag ttagagattt tagaggagtt aaccctaatg
     6781 cttttgatgg tagaggaaat tatgctttag gagttaaaga acaattaata ttccctgaaa
     6841 tagaatatga taaaatagat aaagtaagag gaatggatat aatatttgtt actacagcaa
     6901 aaactgacga agaagctcgt gaattattaa aattattagg aatgccattt tctaagtaag
     6961 caaaggaggg attcccgtgg ctagaaaagc gatggttgtt aagcaacaaa gaaagcaaaa
     7021 gtacgctact agagaataca ctagatgtac tatatgcgga agaccgcatt cagttttgaa
     7081 aaaatttggt atatgccgta tatgctttag agaattagct tataaaggtc aaatacctgg
     7141 tgttagaaaa gcaagctggt agaagcttga ttagaatgga aggagggcaa caaaatgaca
     7201 atgacagatc caattgcaga tatgcttact cgtataagaa atgctaatgt tgttaagcat
     7261 gaaactgtag atgttccagc ttctaatatg aagaaagaat tagctagaat cttattagaa
     7321 gaaggtttca taagaggtta tgatgttata gaagatggaa aacaaggtat cataagaata
     7381 caattaaagt acggacaaga aggcgaaaga gttataacag gtcttaagaa aatatctaag
     7441 cctggaatga gagtttacgc tgctaaccat gagataccaa aagtattaaa cggattagga
     7501 atatcagtta tatctacttc aaaaggaata ttaactgaca agcaagctag aaaagaaaat
     7561 gttggtggag aagtaatctg ctacgtttgg taattaatat gaacaatgta aggaggtgcg
     7621 accatgtcaa gaataggtgt taagccaata ataataccag caggtgttga agttactata
     7681 gctgaaggaa atttagttac agtaaaaggt ccaaaaggaa ctttaactaa acaattaagt
     7741 gctgagttaa acataaaaaa agaagaaaat acaataatgg ttgaaagacc aactgataat
     7801 aaaaaacata gatctttaca cggactaact agaactttat tagataatat ggttgtaggt
     7861 gtaaatactg gtttcgaaaa gaaattagaa ttaaaaggtg ttggatatag agctcaaaaa
     7921 caaggaaaga aattagttat gaacttaggt ttctctcatc cagttgagat ggaagatcca
     7981 gaaggaataa ctgttgaagc tccaaaccaa acagagttaa tagtaaaagg tatagacaag
     8041 caattagtag ggaattatgc tgctaagata agagcttgga gaaagccaga gccatacaaa
     8101 ggtaaaggta taaaatacgt tgatgaagta atcagacgta aagaaggaaa aactggtaaa
     8161 aaataatacc aaaacttaaa cgctagaaag gagtgagcta ggtgttaaaa aaggctgata
     8221 aaaatgctaa tagacttcaa agacataaga gagttcgtag aaaaataagc ggtacttctc
     8281 aaagaccaag attatgtgta tttagaagtg ctaacaatat atatgctcaa ataatagatg
     8341 acacaaaaag agtaactcta gttgctgcat cttctttaga agcagaagtt aagagtgctg
     8401 tcaatcatac aggaaacaag gaagcagcta agaaagttgg agaattagtt gctaaaaaag
     8461 ctgttgaaaa aggaatcact gaagttgtat ttgacagagg tggatactta tatcatggaa
     8521 gaattcaaga attagctgaa ggtgctagag aagctggtct taagttctaa tacgaaggag
     8581 ggaaaaatac atgctacgtc gtaagccgat agatgcagga caacttgact tacaagaaaa
     8641 agttgttgaa gtaagacgtg ttactaaggt tgtaaaaggt ggtagaaatt tcagatttgc
     8701 agcattagta gttgttggag atgaaaacgg acacgttggt ataggtgctg ggaaagcaat
     8761 ggaagtacca gacgctataa aaaaagcagt tgaagatgct aagaaaaatc taatagtagt
     8821 gccaatagtt gggactacaa ttcctcacga agtacgcgga cactttggtg ctggaaatat
     8881 attaataatg cctgctgtag aaggtacagg agtaatagct gggggacctg ctagagccgt
     8941 acttgagcta gctggactaa aagacgttag agctaaatct ttagggtcta acaatccaag
     9001 aaatatggta aatgctacaa tagaaggact taattctcta aaaacagttg aagatatagc
     9061 taaacttaga ggtaaaaaag ttgaagaact tctagggtaa ggaggtagtg taaaatggct
     9121 aaattacaaa taaagttagt tagaagtgtt ataggaacta ctcctaacca aaaaaagaat
     9181 gttgaagcgt taggattaag aaaaagagaa caagttgttg taaaagaaga caatgctcaa
     9241 acaagaggta tgataaataa agtaagtcac ttattagaag taactgaaat agctgaataa
     9301 tttttataaa gtagtgcaag gaggtgcaat ccatgaagtt acatgagtta aaacctgctg
     9361 aaggtgcagt aagagctaag agaagattag gtagaggtac tgcaactgga caaggtaaaa
     9421 ctgcaggacg tggacaaaaa ggtcaatggt ctcgttctgg tggtggagta agagtaggat
     9481 ttgaaggtgg acaaatgcca ctagctagaa gacttcctaa gagaggtttc aataacatat
     9541 ttaagaaagt ttacacagaa gttaatgttg aagttttaaa cagatttgaa aatggaacag
     9601 aaataactgc agaattatta aaatctacta aaactataag caaaataggt aaagacggaa
     9661 ttaaaatctt aggtgaaggt aatttagaga aagctttaac tgttaaagct gctaaattta
     9721 cagcttcagc tcaagaaaaa atagaaaaag ctggtggaaa agcagagtta gtataataac
     9781 ctgctaatct cctagccagt acaggcgggg tgaattagcg tgctgtcaaa attaaaacaa
     9841 gcttggaaga ttaaagcagt aagaaaaaaa gtaatgtata cacttatgat gatcgtgata
     9901 ttcagaatag gtactactat accagttcca ggcatagaca ctagtattat acaaaagatg
     9961 gtaggcggca atagtctact ttctctgtat aatatgttta ctggtggagc atttagtaac
    10021 ttttcactat ttgctttagg tataagtcca tatatcactg catcaatcat aatacaactt
    10081 ctaacagtag gttttgaaag tttagcagaa cttcaaaaat ctggtgaaga aggaaagaaa
    10141 aaaatcaata agtacactaa atatacagca ctagctctag cagttgtaca agctttggga
    10201 attacattag gaattgtaag aagtgcttta atatcaaata gtgtgttttt tatcacaact
    10261 gttgtaataa cattagtttc agcaagtatg ttagtaatgt ggattgggga caaaattact
    10321 gaaaaaggaa tcggtaatgg tagttcagta ataatatttg caggtataat atcaagaata
    10381 cctacagatg ttattaaaat atcgcaacaa gttaaatctg gcgaagttgc accttgggtt
    10441 atagtgattc tagctgtagt tatattactt acagtaacag gagttacatt tatacaagaa
    10501 gcaactagaa agatacctgt acaatatgct aaaagagttg ttggaagaaa aatgtatgga
    10561 ggacaaagtt ctcatatacc aatgaaagtt aaccagtcag gagttatgcc tataattttt
    10621 gctagttcac ttttagcttt ccctcaaaca atagctatgt ttatgggtcc aaatgctcag
    10681 gcttttgttc aaaaatactt aagtatggca acagaacaag gattttggac atatagatca
    10741 attgagattc tacttataat tttcttctca tatttttata caactgtatc atttaataca
    10801 gaagacatat ctaaaaatat gaaaaataat ggtggattta taccaggtat aagaccaggt
    10861 gagcctacta tggattattt aaatagaatt ttaactagat taactttagc tggagctact
    10921 ttcttagcaa taattgctat ggtgccagct cttacaacac attatatgaa agtaaatatg
    10981 agtctagctg ggacttcatt gctaatcgtt gttggagttg ctttggagct taagagacag
    11041 ttagagtcaa acttagttat gagaagctat caaggtttct taaaataaaa tggagatgga
    11101 taatatgaga ataatattac ttggacctcc aggtgctggt aaaggtactc aggcggcagg
    11161 aatagtagaa aaatacaata tacctcatat atcaacagga gatatattca gaaagaatat
    11221 aaaagaggga acagaacttg gaaaaaaagc taaagaatac atggaccaag gtttattagt
    11281 accagatgag ttaactgtag gtttagttac tgatagaata tctcaagaag attgtaaaaa
    11341 tggatttatg ttagatggat ttccaagaaa tgtagcacaa ggagaacatt tagatatctt
    11401 cttaaaaaat gctggtatat cactagataa agttgtcaat attgaagttg ataagagtat
    11461 attagtgtct agagcagttg gtagaagaat atgtaagtct tgtggagcta cttaccatgt
    11521 tgagtttaat cctcctaaag tagaaggtgt atgtgatgta tgccaaggag aattatatca
    11581 aagagctgat gataatgaag aaactgtatc taagagaata caagtttatc tagatgaaac
    11641 taagccttta gtagattatt atagcaaaca aggtataata gcagatataa aaggtgatca
    11701 agcaatagat aaagtatttg aagatattgt cgcagcctta ggaagtggaa aataaatgat
    11761 tatcttgaaa tcaaaaaaag aaatagaact tttaagagaa gcaggtaaaa tagttgctga
    11821 cactcatgaa gttttaagaa aagctatttc tccgggaata tctactttag aattagataa
    11881 aatagctgaa gagaatataa gaaaatacaa tgcagagcct tcttttaaag gatatggagg
    11941 ttttccagga agtatatgtg cttctatcaa tagagaggta gtacatggta ttccaggaga
    12001 aactatatta caagaaggtg acattgtaag tttggatata ggagcctatt ataaaggata
    12061 tcatggtgat tctgcgaaaa ctcacggagt aggcatgata tctgaagaag ataggaaatt
    12121 gatagaagta actagagaaa gcttctatga aggaataaaa tttgctaaat taggatatag
    12181 actttcagat atctcacacg cagtacaaac acacgttgag aaacatggtt tctcggtagt
    12241 tagagattta gtgggtcatg gagttggagc aaatcttcat gaagacccac aagtacctaa
    12301 ttatggactc ccaggaaaag gaccaagact tagagaagga atggttattg ctatagagcc
    12361 aatggttaat gcaggtcgtt atcatgtaaa gactctatca gatgggtgga cgactgttac
    12421 aatagatgga aaaaaatcag ctcattatga gcatacgata gcaattactg aacatgaacc
    12481 tttgatattg acaaagttgt aatttaaaaa gtaggtgaaa aaagtgctat cagataattt
    12541 aagtataggt caagttgtta aagtttcatt aggacgagat aaaggaaatt tattctttgt
    12601 agtcaaaata attaataatg aatatgtatt aatagctgat ggaaaaaaga gaaaacttga
    12661 caaacctaag ttaaagaaag tgaagcactt aaaaaaatat gattttatta atgatgaagt
    12721 tagaaaaaga gtagtatctg gacaagagat tacagattct tttttaagag ctgaacttac
    12781 taagttaaat tagataattt aacttattga aatggagggc tggttagtta atggccaaaa
    12841 aagatgttat agaattagaa ggtacagttt cagaagcttt acctaatgct atgttcaaag
    12901 ttaaactaga aaatggacat gagatattat gccacatttc tggaaagcta agaatgaact
    12961 tcataagaat tcttgaaggt gacaaggtga atgttgaact ttctccatat gaccttacaa
    13021 gaggaagaat tacttggcgt aagaagtaga ctatattaat atttagtcta aggagggatt
    13081 aataatgaag gttagaccat cagtaaaacc aatatgtgaa aaatgtaaag taataaaaag
    13141 aaaaggaaaa gtaatggtta tctgtgaaaa tccaaagcat aagcaaaagc aaggataaga
    13201 aattattact agcattttgt taaattttat agtataatac tatattgtga tgttaacaaa
    13261 agaacgttaa aatcgttgaa caatctggcg taggattggc atatgatgtg aagttttaaa
    13321 cactaaaaac tatgtgtagg aggtgcgatc atggcaagaa tagctggggt agatttacct
    13381 agagaaaaaa gagcggagat aggcttaact tatatatatg gtataggtaa agcaactgct
    13441 aacgaaatat tagctaaggc tgagataaat ccagatacta gaataaaaga tttatcagaa
    13501 gatcaagtta atgagttaag aaaagttata gatgacgatt tcttagttga aggtgactta
    13561 agaagagaaa ttgctttaaa cataaagaga ttaagagata taaaatgtta tagaggaata
    13621 agacacgcta aaggtcttcc attaagagga caaagaacta agactaatgc tagaactaga
    13681 aaaggtccta gaaaaacagt atctcgtaaa aagaaaaaat aaataaataa taactagatg
    13741 aaggagggaa aaggaaaatg gctaaaccaa aaaagaaagt tacacgtatt agaagaagag
    13801 aacgtaaaaa tatagaacgt ggtcatgctc atatacaatc aactttcaat aatacaataa
    13861 taactttaac tgacgttcac ggaaatgcta tatcttgggc aagttctgga caattaggat
    13921 tcaaaggatc aagaaaatca actccatttg catctcaaat ggctgctgaa acagctgcaa
    13981 aagctgcgat ggaacacgga ctaaaaagtg ttgaggtatt cgtaaagggg ccaggttcag
    14041 gaagagaagc tgcaataaga gctttacaag caactggact agaagtaact atgataaaag
    14101 acgttactcc aatcccacat aacggatgca gaccaccaaa gagaagaaga gtgtaattaa
    14161 taagagtaca ataggaggtg tagataaatg gcaagatata caggtgcatc atgtagacaa
    14221 tgccgtagag agggaatgaa attattcctt aaaggtgata gatgttatac agacaaatgt
    14281 gctatagtaa aaagaaacta tgctccaggt caacatggcc aaggaagaaa gaaagtttct
    14341 aactatggat tacaattaag agaaaaacaa aaagttaaaa gaatatatgg agttttagag
    14401 actcaattta gaaatttata tgaacgtgct gaaaatatgc ctggtaaagc aggtgagaac
    14461 ttattaagtt tattagagag aagattagat aatgtagttt acagaatggg actagcatct
    14521 tctagaaaag aagctagaca attagtgact catggtcatt tcactttaaa tggaaataaa
    14581 gtagacatac cttcattaat agttaaggtt ggagatgtta tagaagttaa agagaaatca
    14641 agatcttctg caaaattcaa aaatttagta gaagttaact caagaatagc tcctaagtgg
    14701 ttagaagcta atgtagaagg aatgacagca aaagtagttg gtgttccaac aagagaagat
    14761 atagatcttg agatagcaga gcacttaatc atagaacttt actctaaata ataagcacac
    14821 ttttaaaaat tattttaaaa ttttgtttac cctcagtgga ttactgaatt ttaaggaggg
    14881 ttttgtccat gatagaaata gaaaagccaa aagtagatat agttgagctt agcgaagact
    14941 atagatatgg taagtttgtt atagagcctc tagaaagagg atatggaata actataggta
    15001 acgcattaag aagaatatta ttatcatcat taccaggtgt agcagtgaat gctataaaaa
    15061 tagatggagt tcttcatgaa ttttcaacaa tacctggtgt taaagaagat gttactgaga
    15121 taatattaac tttaaaagag ctttcagcaa ctatagatgg tgaaggaagt agaacactta
    15181 aaatagaagc tcaaggacca tgctctatca caggtgcaga tataatctgt cctccagatg
    15241 ttgaaatatt aagtaaagat ttagcaatag ctacattaga tgataatgct aaacttaata
    15301 tggagatatt tgtagataaa ggtagaggtt atgtttctgc tgaagaaaat aaaacagaga
    15361 atgttccaat aggtgtttta cctgtggatt caatatatac tcctgttgag aaggttagtt
    15421 atcatgtgga aaacacaaga gttggtcaaa aaacagatta tgataaatta gtactagaag
    15481 tttggactaa tggtagtata aatcctcaag agggaatatc attagctgca aaggtattag
    15541 ttgagcattt aaacttattt atagacttaa ctgaacatgt aagtagtgtt gaaatcatgg
    15601 tagaaaaaga agaagatcaa aaagaaaaag ttcttgaaat gactatagaa gaattagatt
    15661 tatcagttag atcatataac tgtttaaaga gagcgggaat taatacagtt gaagaattag
    15721 ctaataaatc tgaggatgat atgatgaagg ttagaaacct aggtaagaag tcattagaag
    15781 aggtaataca aaaactagaa gaacttggat tgggattaaa accaagtgaa gaatagcgag
    15841 caaaggaggg ataagaatgg ctaagtaccg taaattagga cgtgaaacag ctcacagaaa
    15901 ccttatgtta agaaacttag taacttgctt actaagaagt ggaagaatag aaactacagt
    15961 gactagagcg aaagaaactc gtagaatggc tgaaaagatg ataactcttg ctaagagagg
    16021 agatcttcat gctagaagac aagttttagc ttatgttatg gatgaaacag tagttaataa
    16081 cttattcaca gatttagctc caaaatatgc tgagagaaat ggtggataca ctagaataat
    16141 aaaaataggg ccaagaaaag gcgatgctgc tgaaatggct tttatagaat tagtatagta
    16201 aggaaaggat taggcttgat ggcttaatct tttttttatt tttttgacta aggtatgata
    16261 taataatgtg taaaatgatt tttataagtt attgcttact aaaaggagta tgagttaatg
    16321 gataatatag taaaggtaaa taatatttcg tttgagtata ttacagatga agcaaaactt
    16381 aaagcaatag ataatttaag cctagatgtt aaaaaaggag aatttgttgc gataatagga
    16441 cataatggtt ctggtaagtc tactttatct aaaaacttaa atgctattct tatgcctact
    16501 gaaggaaata ttcttattga tgatatggat actaaagaag aagaaagatt atgggatata
    16561 agacaaactg caggcatggt atttcaaaat ccagataatc aaatagttgc tactatagta
    16621 gaagaagacg ttgcatttgg acctgagaac ttaggtatag aaccaaaaga aataagacga
    16681 attgtagagg agtctttaaa aagtgtaggt atgtatgatt taagagatag acagcctcat
    16741 ttgttatcag gaggtcaaaa gcaaagagtt gcaatagcag gaattattgc tatgaggcct
    16801 aaatgtataa tatttgatga agcaacagca atgttagatc catctggaag aaaagaagtt
    16861 atgaaaacaa taaaaagact taataaggaa gaaaatataa ctgttataca tataacgcac
    16921 tttatggaag aagcagtaga agctgataga gttgtagtta tggaaaaagg aaagaaaatc
    16981 ttagagggaa ctcctagaga agtattcagt aaaataaaga tgttaaagga aatcggttta
    17041 gatgtacctt gtatgacaga gttatctagt ttattaatag aagaaggtat aaatattagt
    17101 agtgatatat taactgtgga tgagatggtg atggaattat gtcaattata gtaaagaatt
    17161 taacacatat atataatgaa gggatgcctt ttgcaagtaa ggcacttgat gatatttctt
    17221 ttgagattaa agatagagat tttgttggtc ttataggtca tacaggctca ggaaaatcta
    17281 cattaataca acatttaaat ggattactaa agccttcctc aggagaaata tttataaatg
    17341 attttaatat aacagataaa aacttgaatt taactgagat tagaaaaaga gtaggggttg
    17401 tatttcaata tccagaatac caattgtttg aagaaacgat tgataaagat atagcttttg
    17461 gaccctctaa tttaggctta gaggagtctg agatacataa cagggtaaaa gcatcaatgg
    17521 aagctgtagg acttgattat gagggattta aagacaaatc tccttttgaa ttatctgggg
    17581 gacaaaagcg tagagtagca attgctggag ttatagccat gaacccagaa gttctcatat
    17641 tggatgaacc aactgctggt cttgaccctg gtggaagaga tgaaattttt aacttaataa
    17701 aagacttaca tgaaaaaaag aatatgacta taatactatc atcacatagt atggatgata
    17761 tggcaaagtt agcaaaaact ttaattgtta tgaatcatgg tagcgtagaa tttatgggaa
    17821 ctcctagaga agtgtttaaa tcaaatgcta gtaaattaaa agatatagga ttagatattc
    17881 cacaagtttt agagcttgca ttgaaattga gagaaaaagg ctttgacatc agtgaggata
    17941 tattgacttt agaagaagca aaacaggaaa tattaaaagt tgtgagagga cgaggattat
    18001 gttaaaagat ataactatag ggcaatacta cccaacaagt tctgctattc ataaattaga
    18061 tccaaggata aagcttgtag cgacaattgt atttatggta tctatatttg tagtaaataa
    18121 attttggcct tatatagtag ttttattatg tttattagct atgataaaat tggctaatat
    18181 accagtcaaa tatattgtta agggtgttaa acctcttaaa tggattattt tgtttacatt
    18241 tcttataaat atttttttct taccaggtga tgaaatatgg tcatttggat ttttagctat
    18301 tacaaaacag ggtctaagac aagcaatttt tatggcaata agattaatat ttttagtagt
    18361 tggtacatca ttgcttactt taacaacttc accaatagaa ttaactgatg gaatagagag
    18421 attgttgaat ccatttaaga aaattggttt gccagttcat gaacttgcaa tgatgatgac
    18481 aattgcttta cgttttattc ctacattgtt agatgagaca gacaaaataa tgaaggctca
    18541 gatgtctaga ggtgcagatt ttgaaagtaa gaatcttata aatagagcaa agaatttagt
    18601 accacttttg gtacctttat ttgtaagtgc atttagaaga gctgatgaat tggctatggc
    18661 catggaggcc agatgttaca gaggtggaca taataggact aagatgagag agtctgttat
    18721 atcaagaagg gattatatgg catgtgtatt tcaggttgtt tatctgggag caattatagc
    18781 tactagattt atagcaattt aattaataag ttatagatat aatgaggtaa agtatgagaa
    18841 atataaaaat taaaatccaa tacaatggta agaattattg tggatggcaa aagcaacctg
    18901 attctttagg gatacaaggt acaattgaaa gggctatata tgatataaca aaagaagaaa
    18961 ccagtcttat agggtctggt agaactgatt caggagttca tgctattggt cagattgcaa
    19021 attttaaaat taattcaggt ataagtatag aaagtatacc aatggcacta aatgccaagt
    19081 tgccaaaaga catatcagtt atagaagcat gtgaagtaaa tgatgatttt cactccagat
    19141 acagtgcaaa aggtaaaaca tataaatatt tagtatataa tagcaagttt agaaatccaa
    19201 tacttagtga gatatcatat caagtaaaat atgaacttga ttttgataaa atgtgttcag
    19261 aagcaaaaag tttattagga acacatgatt ttaaaggttt tatgagttct ggttcatctg
    19321 taaaagatac agttagaacg atatatgata ttgatataag taaaaaagat gacttgatta
    19381 cttttgagat tagtggtaat ggttttttat ataacatggt gaggattatt gttggaacac
    19441 ttgttgatat gggtcgtggc agaattaacg aaccattttt agatataata caatcaaaaa
    19501 ctaggtcaag atgtggacat acagctccag cacaaggatt atttctcaaa aaagttcatt
    19561 attaacttga cacaccaggc tacatgtatt aaaatatatg agaatgtgtt aataagtcca
    19621 cttgccccgg acttgacata aacggtaaaa aaattttatg aaaagtagtt aaggagggac
    19681 agttatgaaa agttatatag ctaagccagc tgatgtacaa agaaaatggt acttagttga
    19741 tgctgaagga aaaacattag gtcgtttagc aacagaaatt gcgacagtat tgagaggtaa
    19801 acataaacca acatttactc ctcacgttga tggtggagat tttgttgttg tagtaaatgc
    19861 agaaaaaata gttttaagtg gtaagaaatt agatcaaaaa tattacagat accatactgg
    19921 ttatgtaggt ggattaaaag aaatatctta cagagatatg atggataaga aaccagaaga
    19981 agttatatca catgctgtaa gcggtatgtt acctaaaaat aaattaagaa gcagaatgat
    20041 gactagatta agagtatttg caggtgctga gcacactcat gcagctcaaa atccagaagt
    20101 tttaaacttt aaataagatt aataggtgaa aggaggagtt tataatggct aacgttcaat
    20161 attatggaac tggaagaaga aaaagttctg ttgctagagt aagactagtt gcaggtgaag
    20221 gaaatatatt agtaaatgga agagcattag aaaattattt taattatgag acattaataa
    20281 gagatgttaa acaaccatta gttttaactg gtaatgaaaa taaatatgat gttatagtaa
    20341 aagttgaagg tggaggattc actggacaag ctggagctat aagacatgga atatctagag
    20401 ctttattaaa agctgattta gatttaagac ctgctttaaa gaaagaaggt ttcttaacta
    20461 gagatgcaag aatgaaggaa agaaagaaat acggattaaa agcagcaaga agagctccac
    20521 aattctcaaa aagataatta cttatttttt tggaaaagca aaatccttta tttactgtta
    20581 ttcagaaagt attggtattt taaaaatgtt gggttaagtt aattattgat atataataaa
    20641 ataataagat atcttattag gatgtttttc attaagaaga acatcctttt tattttgact
    20701 atttaataaa aaacatatgc taataacatt taaattttat ttttataata ttgtaattta
//
LOCUS       DAMJVX010000059        20469 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMJVX010000059 DAMJVX010000000
VERSION     DAMJVX010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.062"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            444..1514
                     /locus_tag="PC407_001901"
     CDS             444..1514
                     /locus_tag="PC407_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2497347.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGKLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1498..2367
                     /locus_tag="PC407_001902"
     CDS             1498..2367
                     /locus_tag="PC407_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2497348.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2411..3271
                     /locus_tag="PC407_001903"
     CDS             2411..3271
                     /locus_tag="PC407_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2497349.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSMEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLILQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYIPLIIPFMTS
                     SFGIFLLRQNFMQISDETIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3302..4606
                     /locus_tag="PC407_001904"
     CDS             3302..4606
                     /locus_tag="PC407_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2497350.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INRDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4669..5931
                     /locus_tag="PC407_001905"
     CDS             4669..5931
                     /locus_tag="PC407_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDF2497351.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKVKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFDLKLDGVEKEAFDFIM"
     gene            6118..8088
                     /gene="uvrB"
                     /locus_tag="PC407_001906"
     CDS             6118..8088
                     /gene="uvrB"
                     /locus_tag="PC407_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDF2497352.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8107..10932
                     /gene="uvrA"
                     /locus_tag="PC407_001907"
     CDS             8107..10932
                     /gene="uvrA"
                     /locus_tag="PC407_001907"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2497353.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11191..13008
                     /gene="uvrC"
                     /locus_tag="PC407_001908"
     CDS             11191..13008
                     /gene="uvrC"
                     /locus_tag="PC407_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDF2497354.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVEAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13008..13937
                     /locus_tag="PC407_001909"
     CDS             13008..13937
                     /locus_tag="PC407_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDF2497355.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14151..15947
                     /locus_tag="PC407_001910"
     CDS             14151..15947
                     /locus_tag="PC407_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HDF2497356.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQILSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLSPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVISHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16087..17868)
                     /locus_tag="PC407_001911"
     CDS             complement(16087..17868)
                     /locus_tag="PC407_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2497357.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRICMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17891..19777)
                     /locus_tag="PC407_001912"
     CDS             complement(17891..19777)
                     /locus_tag="PC407_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDF2497358.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19794..20291)
                     /locus_tag="PC407_001913"
     CDS             complement(19794..20291)
                     /locus_tag="PC407_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HDF2497359.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLTTKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
BASE COUNT         7913 a         2718 c         3503 g         6335 t
ORIGIN      
        1 ggtaaattta taaaaattaa aaaagtttta ttttttatgt ttaaaccttg taaatttggg
       61 acataatata attgtaaaac ttaatatagc aaaaataaat tactcaatct cccccactta
      121 aagacctcta tcccccatag aggtcttttt tattttcaaa ccaaattata taaaaaataa
      181 atagttttat atgactcttc ataaattaaa atcacattgt gatagcaaac catgaatatg
      241 aaaaatatag aaaattatag ctaacataag ctgtatatca tcttaacttc agtatattta
      301 acaaaaaatg tttataattt aacctttatt ttaataagtt tactatatat tcactttgtc
      361 ttaatatatc ctttacaaat taagagtatt attaatctat aagtaaatcg tagaattaaa
      421 ttaaatattt tgggaggata aaaatggcag atataagact agaaaatgta acaaaatctt
      481 ataataaaaa aaatacagtt atagaaaatt taaatcttac tatagaagat ggttctttta
      541 cagtattggt aggtccaagt ggatgtggta aatctactac attaagaatg attgcaggtc
      601 ttgaagatat aacttcaggc aagttaaaaa ttgaagaaaa tgacgttaca aaaacggatg
      661 ctagtaaaag ggatatagct atggtatttc aaaattatgc attatatcca catatgactg
      721 ttagagaaaa catagaattt ggtcttaaaa acaaaaaagt agaaaaagaa aaaagagaaa
      781 aacttataac agaagttatt gatgtagtag gattgagaga atatttaaat gtgaaaccag
      841 gcaacttatc tggaggtcaa agacaaagag tggcacttgc tagagctatg gttaaaaatc
      901 caaaggtatt tttaatggat gaaccattgt caaatttaga tgctaaatta agaaatcaaa
      961 tgagaacaga attaatacaa ttacatcaaa aactaaaatc tacatttgtt tatgtaactc
     1021 atgaccaggt tgaggcaatg tcaatggcag ataagatagt tataatgaac aaaggaaaga
     1081 taatgcaaat aggttctcca aagaagatat atcaaaatcc taagaattta tttgtagctc
     1141 aatttatagg gagtccttgt atgaacatac tagatataaa aaatgatatt aaatttggat
     1201 tcagaccaga aaaagcaaca ctagagagta taaacataga tggaaaaaat gatgatgtat
     1261 ttagcctatt tggaaaagtt gtgactaaag aagtgttggg aaatgaaact atatattgtt
     1321 tatctgttct taatagagaa ataagagtca aaacggaaaa tgatatattt gatattggaa
     1381 gaactttaag aataagtgta ttagaaaaag atttatatta ttttgataaa aatggagaaa
     1441 gaatagaaga tgtaaaagta tgtaaaagta tgtattctaa aattaaagga gatatttatg
     1501 aaaaagcaat ttaataaagc atatttatat ataacacctg ctattatagg aactttattg
     1561 tttatagtct atccaataat taagacgata tctctaagct tcaaatctgg tagtcttctt
     1621 agttcaacct ttgaaaatgt aggcttaaac aattatacag aacttttaaa gaatccagag
     1681 tttatacaaa ctataacgaa cactgcaatt tatacttttt ttatggtgac tatatctata
     1741 tcactagcca tagtacttgc tgtttggcta aataaaaatt ctataataca caatttaacg
     1801 caaagtataa tctttacacc acatgtaatt tcactagtat ctgtagctgt attgtggctt
     1861 tggattatgg agccacaata tggcttttta aactggatac tatcgacatt acatttacca
     1921 acatcaaagt ggctttcaag tgaaagttca gctttaatgt cactaattct aataggaata
     1981 tggaagactc ttggatacaa tatcttaata gtattatcag gtcttaaatc cataccaaac
     2041 tatatatatg aagcatctaa attagataat gcaaacaaaa taacgaattt ctttaaagtg
     2101 acattgccgc tattatcacc aacaatattt tttcttatga taacaacaac aactgcatct
     2161 tttcaagtat ttgatgagat aagtataatg acacaaggtg ggccagtggg ttcaactaac
     2221 atgttatctt attatatata tgagtctgga tttatatact atgacatagg gcaagcgagt
     2281 gcagcgagta tgtttttgtt agctatagtt atgattgcta catatgctaa ctttagactc
     2341 ttatcaaaga aagttcatta tcaataaaga aaaagtagat attataagat ttatccaaaa
     2401 ggaggagaca atggatatta tacaacctat caaaaaagac aaagttgata taaaaaaaat
     2461 agcatcaaaa tcaatggaaa tattaattct attactttta tctgcaatat ttatattccc
     2521 ttttatatgg atgctatcga catcctttaa aaatccacaa gaaatgatgc aattaccacc
     2581 tactattata ccaaatgata tagtgttaga aaattatagt acagcatggc attcaggtcc
     2641 attttttaga tatttactaa acagtgtatt tgtaacagga agtgttttaa ttttacaatt
     2701 tttagttatg gttccagcag catatgcatt ttctaagatt aaatttaaag gtgaaaaaat
     2761 gttatttggg cttttgcttg taggacttat gattccacca caagtaactt ttctaccagt
     2821 ctatatgatg atgagtaagt ttggatttat aaatacttat attccactta taattccatt
     2881 tatgacatct tcatttggga tatttctgct tagacagaat tttatgcaga tttcagatga
     2941 aactattgaa gcagctaaac tagataatgc ttctgactta aaaataatgt ttagaataat
     3001 ggttcctatg gcaaaaccag cagtaataac atttatatta tttaatttta tataccattg
     3061 gaataattat ttctggccct tagtaatgac aaatacagat gcaataagaa ctttaccaat
     3121 aggagttgcg ctccttaaat cttcagaagg aattactgca tggaatgtaa taatggcagg
     3181 aaatataatt ctaattttac caataattct tgtttatata tttgcgaata aaaaagtaaa
     3241 agaagctttt atgtattcag gaataaaata aaaaattaaa gactaaattt ggaggatgaa
     3301 aatgaagctt aaaagaaaat tagcaacagg attagcaata acattattat taggttcttt
     3361 agttgggtgt tcaaattcta atgatgcaaa tgcatctaaa ggagaaaaga ctgatagcaa
     3421 tattacgaag atagatttct gggctccttt aggtggaact aatggagaaa ctgctcaaaa
     3481 aatggttgac caatttaata gtgaacataa ggatgtacaa gtaaatatgt taaaacagaa
     3541 agattactat gaaaatgcaa caaaactaca ggcagcctta acatctaaag accaaccaga
     3601 tgtaacatta ttagaaataa ctcaaacagg tacattcgca tcagcaggtg ctttagtaga
     3661 tatgagtaaa tattttgata aaacatatca agaaagattc ttttctggtc ttttaacaaa
     3721 ttcatattat gaggataagt ttgttggtat gccatttaat agaagtacac caatattata
     3781 cataaataga gatatggcaa ctaaagcagg tcttgaccct tcaggtccaa aatcatggga
     3841 agagcttaag gattacgcta gtaaaatgac aaataaatca gaagatactt atggttttga
     3901 aacaccaata gatatttggt tctatgaagc tatggtaatg caaagtggag gagaaataac
     3961 taatggtaaa aaagtagcat ttaataatga ggcaggacaa gcaccagtta aattctggca
     4021 agatatgatg aaagctggaa taatgaaaat gcctcctgga gaagattata atgcctggga
     4081 cgtagcaaaa caagattttg taaatggtaa agtaggtatg atatttacat caacagcaga
     4141 tttagcagga cttatgcaac aaacagaagg taagtttgaa atatcaacag cattcctacc
     4201 aaaaaatcaa aaatacgcaa ctcctacagg tggagctaat ctagttatgc ttgaaggtgg
     4261 aacagataaa gaaaaagaag caagtgcaga atttatagaa tggatgacac aaacagataa
     4321 aatcgttcag tttagttcat caacaggata cttaccaaca actgaagatg caacaaactc
     4381 agaaaagtta caaacattat ataaagaaaa acctcaatat aaggttgcaa ctgaccaatt
     4441 acaatatgct gttgcacttc caatgttaaa tggatacaag gaagcaactg ataagctaat
     4501 ggatgagatt aaaaaggggc ttactgattt aaatgcaaat ccaaaagatg tagtaagcaa
     4561 aggaacttct gcaatgcaag cagtaataga taaaacaaat aaataattat atagacttgt
     4621 aagttgatta aaagtattca ataatatata aatgaagagg taatgtaaat gacaaaggtt
     4681 atagtattat cagtagattc tttatttgaa aaagacttgg aatttgtaaa aaatctacca
     4741 aactttaaat ctatactaga aaattgctcc atagtaaaaa atataaattg tgtttatccc
     4801 actctgacat atccgtgtca tactagtatg gttacaggag tatatcctaa aaaacatggt
     4861 atatatcata atgaaaaatt tgacccaaat aaagaaaata aagactggta ctggtattca
     4921 aaggacataa aagtaaaaac tattatagat gtagcaaaag aaaataatct aactacctct
     4981 agtgtattat ggcctgtaat gggagcaaat ccaaatatag attataatat tgcagaaata
     5041 tgggctccta gtagagaaga tgacccaaga gaaacttttg aacgaagctc cagtaaggtt
     5101 ataatggacg gtatttacaa tagacattgt cattatattg attggaaatt agaacccaac
     5161 atggatttat ttggagtaaa ctgttcagta gatataataa aggaatataa accagattta
     5221 atgctaatac atttagctac attggaccat acacgacata attatggctt atttaatgac
     5281 gaagtagata aagcattaaa aatgaatgat aaatggctag gagatattat tcaggctaca
     5341 aaggatgcag gtacatacaa agatacaaat tttataatac ttggggacca tggtcatctt
     5401 agagttgata aagttataaa tccaaatgtc ttgcttaaat caaatggatt tataaaagtt
     5461 gaaaatggag aagttaagga ttttgatgcc tatgtaaatt cttctggaat atcagctcaa
     5521 ataatagtaa ataacttaga taggttaaca gaattaagag aactgcttga agaatttaaa
     5581 gaagaattat tcatagagaa tatttttacc aaagaggaag catctaacct tggtcttgaa
     5641 ggtgattttg aaatggtgat tgaaggtctt gaaggaattt catttggaaa tgatttcgaa
     5701 ggaagtatta taaaagattc agatataaaa gattacaagt ttgctgttgc gacacatggt
     5761 catttaccta ctaaaggtaa tagacctcca tttatagctt ttggaccaaa tattaaaggt
     5821 ggtgtggtta tagaagaagg agacctgaga gcacatgcag caactatatt aaaaatgttt
     5881 gatttaaaat tagatggagt tgaaaaagaa gcatttgatt ttattatgta agttgaataa
     5941 agtatttatt ttccatgaat tatagacttc tattgcaaag atagaagtct ttttttatgt
     6001 tttagattta agtaaaattt aaatgggtaa aatatttaaa ataacttaaa caattttata
     6061 aaggtatgat ataataatac atgtcaggaa ctaatgttcg aaaaaaggag tgatactatg
     6121 gattttaaaa taaaatcaga cttcaaacct acaggtgacc aaccagaagc tattaaatca
     6181 atagtggatt ctataaatag aaatgagaaa ttttcgacat tattaggtgt aacgggttca
     6241 ggaaaaacat tcacaatggc aaatatcatt caacaagtta agaaacctac cctaataatg
     6301 gctcataata aaactctagc agcacaactt tacagtgagt ttaaagaatt tttcccagac
     6361 aatgcagttg aatactttgt aagttactat gattattatc aaccagaagc atatgtagct
     6421 cacagtgaca cttatataga aaaagatgca agcataaatg atgaaataga taaacttcga
     6481 cattctgcta cagcatctat tttagagaga agagatacta taataatatc atctgtatct
     6541 tgtatatatg gacttggtga cccaaaagac tataaagaac ttatgttatc tataagacca
     6601 ggtatgcaaa gagacagaga tgatgtaata aaaaggttaa tagagataca atacgaaaga
     6661 aatgacataa actttacacg aggtaccttt agagttagag gagatatact agaaatattc
     6721 ccagctagta atgatgaaaa agcaataaga atagaatttt ttggtgatga ggtagataga
     6781 ataacagaaa ttgattatgt aacagggaag attgttggta ctagaaatca tgttgttata
     6841 ttcccagcat ctcactatgt aacaacgcca gaaagaattg aaaaggcaat tgtagaaata
     6901 gaaaacgagc ttcaagaaca aattaaattt ttcaaggaaa atgataggct attagaagca
     6961 caaagaattg aacaaagaac taagtatgat atagaaatgt taaaggaaat aggattttgt
     7021 caggggatag agaactattc tagacatatt acaggaaggt cagaaggaga aagaccatat
     7081 actttaatgg atttcttccc agatgattat ttgataattg tagatgaggc acatgtcact
     7141 ataccacaag taagaggtat gtatgcagga gacaggtcta gaaaaacatc acttatagag
     7201 aatggtttta gattaccttc tgcacttgac aatagaccat taaattttca agaatttgaa
     7261 ggaaatataa atcaaatgtt gtttgtaagt gctacaccag gaccttatga aattcaacac
     7321 tcagagacaa tagcagaaca gataataaga ccaactggcc ttttagaccc aattgtagaa
     7381 gtaagaccta taaataatca aatagatgat ttggtaggag aaataactaa aactatagaa
     7441 aaaaatgaaa gagtgcttat aaccacttta actaaaaaaa tgagtgagga cttgactaac
     7501 tatcttaaag aaatagggat aaaagttaaa tatttacact cagatatagt tacattagaa
     7561 agaacagaaa taataagaga tttaagatta gggaagtttg atgtccttgt aggaataaac
     7621 ttacttagag aaggattgga tataccagaa gtttcactta tagcaatttt agatgcagac
     7681 aaagaaggtt tcttacgttc cgaaacagct ttaatacaga ctattggacg tgcagctaga
     7741 aatgaaaatg gaagagtaat tatgtatgca gacaggataa ctgactcaat gcaaaatgct
     7801 attgatgaaa caaagagaag aagagatata caaaatttat ataatgaaga gcacaatata
     7861 attcctaaaa ctatacagaa aaatataaga gatagtatag aagctactaa ggttgcagag
     7921 gaagaagttg tatatggaat atctgataca gatgataaag acgaaataag agctaatata
     7981 gataaattaa aatcagagat gatggaagct gctcaaaact tacaatttga aagagcagca
     8041 gaacttagag ataaagtgaa acaattagaa gaaaaattgg aaaaatagaa aggtaaaaac
     8101 aacattatgg aagataaaat tataataaga ggagcaaaag aacataattt aaaaaatata
     8161 gatttagaat taccaagaga taaatttatt gtttttacag gtttgtcagg gtctgggaag
     8221 tcttctttgg catttgatac aatatatgca gaagggcaga gaagatatgt agaaagtcta
     8281 tctgcttatg caagacaatt tttaggtcag atggaaaagc caaatgtaga atatatagag
     8341 ggtttatcac cagctatctc aattgaccag aagactacct caaaaaaccc acgttctacg
     8401 gttggaacag taacagaaat atatgattat ttgagactat tatttgcaag agtaggagat
     8461 gtgtattgcc ctacatgtgg tgaaaaaata agtcagatga ctattcaaga aatagttgat
     8521 aaaatgctag attttccaga tagaacgaaa ttacaaattc tttcaccaat tgttagagga
     8581 caaaaaggta cacataaaaa agtctttgat aatatcaaaa aagaaggatt tgtaagagtt
     8641 agagttaatg gtgagaacta tgaagttagt gatgatataa tcttaaataa aaatcaaaaa
     8701 cataatatag aagttgtagt ggacagaata gtaattaaag atggaataga atcaagacta
     8761 gcagattcaa ttgaaactgc agttaagcta tctgatggtc ttgtaatagc ccaaataata
     8821 gatggagaag aaattctttt ttcaactaaa tttgcatgcc cagaacatgg aattggcata
     8881 gaagaattat ctccaaggat gttttcattt aatgctccct ttggtgcttg tgatgtttgt
     8941 aatggtcttg gggaaagtaa agaagttgac ccagaattag tagtaccaaa caaagattta
     9001 tcaataaaac aaggtgcagt ggcagcatgg ggttcaacag gtgttaatga tgacacttat
     9061 tacagtaaaa tgataaaaag tttagcagag cattttaatg tatctttgac aacaccgttt
     9121 aaagatttac cagatgattt tgtggaggaa ttactttatg gtaaagataa tataattgta
     9181 gagtttactt atgagtcaaa atttggagga actagaaatt ataaatcata ctttgaaggt
     9241 gtaatagtaa acttagagag aagatataga gaaactaatt cagattacat gagagataag
     9301 attgaagaat acatggcaga gagaccatgt ccaaagtgta aagggatgag acttaaaaag
     9361 gaagttttat ctgttttagt tgatggaaaa aatataatgg aagttacaaa cttatctgta
     9421 aatgaattga ttgactttat ggaaaatata aatcttacag aaaaacagag atttatagct
     9481 catgagatta taaaagagat aaaaggaaga gctatgttct taagagatgt aggtcttgat
     9541 tatttaaatt tatcaagaaa agctggaact ttatcaggag gagaagcgca acgtataaga
     9601 ttagctactc aaataggttc tgcattggtt ggagttttat atgtacttga tgagccaagt
     9661 atagggcttc accaaagaga taatgatagg cttataacta ctcttagaca tctaacagat
     9721 ttaggaaata ccttaatcgt tgtagagcat gatgaagaca caatgagaga agctgattat
     9781 gttatagaca ttggtccagg tgcaggtata catggtggtg aaatagttgc acagggtact
     9841 ttagatgaaa taattgaaaa tgagaactca ataacgggtc aatatttaag tggtaaaaaa
     9901 caaatattgc ttcctgagac tactagagaa ggaaatggaa gttttataga aatagttaaa
     9961 gcatcagaaa ataatctgaa aaatattgat gtaaagttcc cattaggtaa gtttacatgc
    10021 ataacaggtg tttcaggttc tggaaaaagt acattaataa acgatatact ttacaaaggt
    10081 gttgcaagta aagtgaatag acttaagcaa agagctggta agcataagga gataaaagga
    10141 attgaaagta tagataaagt tataaatata gaccaaagtc ctattggaag aacacctcgt
    10201 tcaaatcctg ctacttatac aggtgtattt gattttataa gagatatttt tgcatctact
    10261 aatgaagcta aagcaagagg ttataaaaaa ggtagattta gttttaatgt aaaaggtggt
    10321 agatgtgaag cttgtaaggg tgatggaata ataaaaatag aaatgcattt cttaccagat
    10381 gtatatgttc cttgtgaaat ttgtaaaggt gaaagataca atagagaaac attgcaagta
    10441 aaatacaaag ataaaactat atctgatata ttagatatga atgtggaaga agctgttgag
    10501 tttttcgaaa atataccaaa tataaaaaga aaattagaaa ctttaatgga tgtaggactt
    10561 tcatatatta aattaggaca gccttcaact caattatcag gaggagaagc gcaacgtata
    10621 aaattagctg ctgaacttag taaaagacca acaggaaaga ctttgtatat attagatgaa
    10681 cctactactg gacttcatat ggcagatgta gacaagctaa tccaagtatt acaaagacta
    10741 gcagatacag gaaatacaat tatagtcata gaacataatt tagatgttat aaagacttgt
    10801 gattatgtaa tagacttagg accagaaggc ggagataagg gtggaactat agttgcaact
    10861 ggaacaccaa aagaagtatc taaagttgat ggttcttata caggtcaatt cttaaagaaa
    10921 tatttttctt aagttatata aattgtgaaa aattttagag cagatatacg ataaactgtt
    10981 ttgttataag taaattagtc aaatataagt tgtaaatagt aatacaaagt aaagtccata
    11041 ttactcttca tggttaaaat tgttttcaaa tcaattatag aagggaatat ggactttaac
    11101 ttgtctaaag cactggaaat tgtataactt tttaaaattc atattttagg gtataatttt
    11161 agtataaaat ttaatgtttt ggagggcgag atgtttgata tacaagagca tttaaaaaaa
    11221 ttaccttctg agcccggagt atatttaatg aaagacaaat acgaccatat aatatatgtg
    11281 ggaaaagcca tatctctaaa aaatagggta aggcagtatt ttcaatcttc aaaaaaccat
    11341 acatctaaag taaaatctat ggttaagaat atatataagt ttgagtatat tataacggat
    11401 tcagagttag aggcattaat acttgaatgt aatttaataa aaagatacag accaaagtat
    11461 aatgtagtgc ttagagatga taaaacttat ccatatataa aagtaactac gaatgaagat
    11521 tatccaagaa tcttaaaagt tagaagagtt cttaaagata aggcaaagta ttttggaccc
    11581 tataccaata taactgctgt gaatgatacc ttggagctta taagtagtac gtatccaata
    11641 agaagctgta aaatagatat agataaagca ataaagaata agacaagacc ttgtttaaat
    11701 ctacacataa ataaatgttt aggaccttgt acaggaaatg tttctaaaga agagtatgga
    11761 aaaatgatag aagagattat aatgtgttta tctggtaaag aagaaaaatt gatggaactt
    11821 ttaaaggaaa aaatgaatga gagttctatg aactttagat ttgaagaggc tgctgtatat
    11881 agggataaga taaagagcct tgaagagatg atacagaaac aaaaaataga tgctactgta
    11941 agtgacttaa accaagatgt ggttgcaatg gcaagagctc ataatgaagc ttgtgtccag
    12001 gtatttttta taagaaatgg taagattgta ggtagggaac attttatatt agaaggtgta
    12061 atggatagtc caagagcttc tatattaagt tcatttgtaa aacaatttta taatgaacaa
    12121 gagtatatac ctaaagaatt aattatagaa gatgagatag aagatagttc tatattagaa
    12181 gaatggctgt catctaaaaa ggggcaaaaa gtaacaataa gagttcctca aaagggagag
    12241 aagaaaagct tagttgaaat ggttaggaaa aatgctgtag aatatcttga aaaattttct
    12301 gatatgaata aaagaaaata tgaaaaaagt attggagcat tggaagaatt aaaacaaata
    12361 ttaaatctag aaaagttgcc aataagaata gaggcttatg atatatcaaa tatacaaggt
    12421 gtagattcga ttggttctat ggtagtgtat acgaatgcta aaaaagataa gaaggaatat
    12481 aggagatata aaataaagac agttattgga cctaatgatt atgattctat ggctgaaatt
    12541 gtagatagaa gattaaaaca tggaaattta cctgacttga tattgttgga tggtggaaaa
    12601 ggacaagtta gtgcagtaaa aaaagtacta gaactcaatg atgtagatat acctctttgg
    12661 ggaatgtaca aagatgataa gcatagaact aaaggattga tatgcaaaga aaaagaaata
    12721 gaactagata aaacaactaa tttatataga tttattgcta gtatacaaga agaagttcat
    12781 aattatgcaa taacatatca tagaagtcta agaaataaag ctttgacaaa atctatctta
    12841 gatgatatac aaggaatagg tgaaaaaaga aagaaatcat tattaaatca ctttaaagat
    12901 gtagaggcta taaaaaaagc tactatggaa gaacttttag aagtagatgg aatgaataaa
    12961 agcattgcag ataatgttta taattttttt agaaaggaag aaaattaatg gaaactagca
    13021 ataaagtatc aataagagag ataataaaag agttgaagtt aaaagtagta tacatgcctg
    13081 atgatgtaga ttattatgtc tactctcaag atataaatag accaggatta cagtttgcag
    13141 gatattttga ttattttgca tatgaaagaa tacaaatagt tggaaagaca gaatataatt
    13201 tttttggatt aatggatagt catattcgag aagaagtact agatagattt ttctcatatg
    13261 aaataccagc gttaataatt tctagagact tggaaatcaa gccagatgta attgaaaagg
    13321 ctaaaaaata taaaagaata cttttgagca caaatagaaa taccactaga cttataaata
    13381 gactgtcaaa ctatttggat aataagttag caccacacac aacaattcac ggggtgctag
    13441 tagatattta tggtataggt gtactgataa aaggtgaaag tagtatagga aagtcggaaa
    13501 cagcattaga actgattcaa aggggtaata gattagttgc agatgatgct gtagaaataa
    13561 gaaaattaga tgaaagtatg cttgtgggtc agtcacctga actaataaga cattttcttg
    13621 agataagagg aataggaata atagatgtta gaagtttata tggagtaggg gcaatcaaaa
    13681 attctaaaat gatagattta gtagttcatt tagaagcttg ggatgaaact aagtattatg
    13741 atagactagg actggataaa gaatacgaag aaatacttgg tgttaatgta gagaaattag
    13801 ttgtacctgt taaacctgga agaaatactg ctatgatatt agaagtagca gcaatgaatt
    13861 ttagacaaag aggaatggga tacgatgcag ctcaagaatt tactaaaaaa ttatcaaaat
    13921 taatcgatac taagtagtac tgaataatag tgaactacac atcatttaat taaatattga
    13981 ttaggattat gtcctacacc ttataaatta aaatcaagtt gtatatatat aatgtttatt
    14041 aataagatat attatataag gataatttaa agtacagtgt tagattataa taatactaga
    14101 ttaattttat atttgagttt cattatataa caagaaagtt ggaatttttt atgggaatag
    14161 aatattttag taaactttta gaaagtagta ctgaaaaaga attgctctta agtaaaaagt
    14221 caaataacat aagtacagca agattaatat cttttttgat agtgatagca gggtttgcta
    14281 ttggatttta caataaaaat atagtaggta tttttgttgg agttttatca ataataatat
    14341 ttatagcact cttagttatt catagtaaag taaaagaaga agaaacatat tttaaaagta
    14401 agagcgaagt attaaataag tatattaaga gatttggaga tggttggaag gaatttaaaa
    14461 tagatggtaa agaatactta aaagatgaaa attcgcaagc aaaagattta gacttatttg
    14521 gaagagcatc attgtatcaa tatatctgtg tagctaatac atcttatggg aaaaattttt
    14581 tagccaaata tttgtggaat gaaaatccag atgagaatgt aatcttagaa agacagaggg
    14641 ctataaaaga acttttatca aaagatgatt tttctattca tattcaaata ctcagtaata
    14701 taattggtaa agagcaaaaa aataattcag atagtagtat agaatcgttt atagagtatg
    14761 gagaaaataa gaatgtgtat ataccaaaat ggatgcatat ttttacttgg ggtctaccaa
    14821 ctgcaacaat cttatctttt atattttgta tgttaggatt tttacctgtt ttacctgttt
    14881 ttttactttt tataatccag ttagggttta gtggttttgg atatccaaga cttatgcaaa
    14941 ctctttctcc attattttca tttagtagaa gtatacaagt atatgaaaaa atgtttgagg
    15001 ttttagaaaa agagacgttt gagagtgcat atttgaaaga attacaagat aaactttcta
    15061 agggaagtgg agtttcaaga ggtgttaaac aattaaattc aataggaaat gctgtaaacc
    15121 taaggtataa tcagatacta tatataattg cctgtggtgt tttgatgtgg aactatcatt
    15181 gtgcagaagc tttggagcga tggaaaggta tatatggaaa tcaaattaga gattggtttg
    15241 aatctattgg agaatttgaa gctctaataa gtttaacagt tatttctcat gtaaaggaaa
    15301 atacatgttt tccagtaata aaatatgaag atactccaag attgaaagtt gaagaggtat
    15361 accacccatt aatagcagaa aaaagtgtca tagcaaattc gatacaatta aattctcaga
    15421 cttgtattat cacaggttca aatatgtctg gaaagacaac atttttaaga agtatcggtg
    15481 taaacttagt gctggcatat gcaggtgctc cagtatgcgc taagagtttt gatgcgacct
    15541 gtatggctat atttacttct atgagaatac aagatgatgt aagtcaagga atatcaactt
    15601 tctatgctga gattcttaga ataaaatcaa tgattcaata tagtttaaaa gaactgccaa
    15661 tgttggttct agtagatgaa atttttaagg gaactaactc tgctgataga attattggag
    15721 caagtgaggc agtaaagaaa ttatcaaaac cctgggttat aagtatggtg acaacacatg
    15781 actttgagct atgtgacctt tcaggtagtg gagatgttga agttgtaaac tatcatttct
    15841 cagagtacta tgtagacgat aaaattcatt ttgactataa gataaaagat ggaagatgta
    15901 aaactacaaa tgcaaaacaa cttatgagaa tggcaggtat tctttaatta atagataggt
    15961 taattaaaga ataagtctat ttagaaatat aatttaattt gtgaaattaa taaggattgt
    16021 cttaaattac gtaaaagtat tttaagacaa tcctttttta agaataaatt ttatactttg
    16081 ttatgactac ttagcttctt cacaagcaca ttcttgtgga cactctccat agacacaact
    16141 cttatcaaag taatgagtat gaaggtattt atgggctaca tcactatttg gttctccaaa
    16201 gaagtcttta taaatagctt gtaaatcagg attctcatga gatttacgaa tagccttatt
    16261 tctatctatt tcatataacg catttgcacg tctatcaact atttcagtat taccatggtg
    16321 ataaggttga ccagcacctc caacacatcc accaggacat gccataactt ctatgatatg
    16381 gtatttacat tctccacttc tgaccttatc cataatcttt ctagcatttt taagtgtatt
    16441 tacaatacac acattaactg tagttccatc aacatctata cttgcttccc taattccttt
    16501 aagacctcta acattagtga agtttacatt atctaaagtc ttattagtta tcttctcata
    16561 tgaagtacgt agggcagctt ctaaaactcc accacttgct ccaaatatag atgcagcacc
    16621 tgtagatttt cctagaggat tatcaaaatc ttggtcttga agattaggta aatcgatagc
    16681 agcttctttt atcattttag ctaactctct agtagttata gaaaggtcaa catctagtat
    16741 tccagattca cttaattctt ctcttgaagc ttcatatttt ttagccacac aaggcattac
    16801 agatacaaca taaagttctt caggattgat tcctaatatt ttaggtgcat agtagttttt
    16861 agctatagaa ccaaacatac cttgaggaga cttacagcta gatgctaagt ttaacttgtc
    16921 tggataattg tgttctaaga agtttaccca tgcaggacaa caacttgtta atataggaag
    16981 attttcacct ttttggattc tttctataaa ttcagtagct tcttccatta tagtaaaatc
    17041 tgcaccaaag tttgtatcaa atacatgttc aaatccaagt gcttttaaag cagctaccat
    17101 ttttcctgta gagattgacc ctggttctaa tccaaactct tcaccaatag caactcttac
    17161 agcaggagca acttgcacta ctatagtctt ttctttttta tttaaaacat cccaaagttt
    17221 aggtacatta tctacttctg ttaatgctcc agttggacag acagatatac attgaccaca
    17281 gaatgtacac tctgtttcaa ccatatcaga gttaaagaat gtactaacta aagtattaaa
    17341 tccacgatta actccagata atgctcctac tgtttgtatg tcattacata cagtctcaca
    17401 tcttctacat aatatacact tagaatggtc tttaactaaa gatttagtag aagtatcttt
    17461 acctgcaaaa gatttggaac cttgatatct tatttttctt actcctaaat cagcagctat
    17521 agtctgtaat tcacaatctc catttttttc acagacaaaa caatcttgtg gatggtctga
    17581 taacaataat tcaacaatag ttctacgagc atttaatgct ttagcactat tagtctgaac
    17641 tttcatacct tcctttataa ctgttccaca agctggaact aagcctctct ctgtctcaac
    17701 catacaaata cgacaagaag cacaagtgtc aagcttatct atttcgttca tatgtaaatg
    17761 acaaaggttg tgtattttta tatttattaa tttcgcagca tctaagatag aagttccact
    17821 aggtgctgaa acgtgtttgc cattaatagt taaattaact aaactcattg ttattcctcc
    17881 tccaaataag ttatttcttg attatagcgt taaatgtaca attatctata caagcaccac
    17941 attttagaca cttagaagta tctattgtat gttgttcctt aacagaacct gttatacaac
    18001 cagcaggaca cactctagaa cattttgtac aacccttaca gtcatctgtt ataaagtaag
    18061 aaagtaagtt ctgacattta ccagcaggac atttcttatc tacaacatga gctatatact
    18121 catctttgaa atattgaaga gtacttaaaa cagggtttgg agcagcttta ccaagaccac
    18181 ataatgaagc agtttgtata gtttcagcaa ggttttctag gtcatctaaa tcttgtagag
    18241 taccattacc ttctgtaatt ttagttaata tttctaataa tcttgtagta cctatacgac
    18301 aaggtgtaca tttaccacaa gattcttcaa cagtaaactc aaggaagaac ttggctatat
    18361 ctaccataca gtcagattca tcaagaacaa gcataccacc agagcccatc attgaaccga
    18421 tagaacttaa tgaaccaaag tctataggag tgtctaaatg ttcagttggt atacatccac
    18481 cagatggtcc acctgtttga acagccttaa atttcttacc ttcagggatt cccccaccaa
    18541 tttcaaatac aatatctctt aaagtagtac ccattggaac ttcaacaagt cctacatttt
    18601 ctacttttcc accaagagca aagactttag taccactaga atcttcagta cctaagttgg
    18661 caaaccaatc tccaccctta agtataattg caggtatgtt agcaaaagtc tcaacgttat
    18721 ttaagcaagt aggagatttc catagaccac tcttagaaga actataagtt ttcattctag
    18781 gttcaccacg tctaccttca attgaatgca ttagcgctgt accttcacca caaacgaaag
    18841 cacctgcacc atattttaat tctaatttaa agttaaatcc agtgcctaag atattttcgc
    18901 caagtaaacc gtatttttca gcttgagcta tggctacttt taatctttct atagattttg
    18961 gatattctgc tcttatgtat atgtatcctg tatcagaacc tatagcgtat ccacatattg
    19021 ccatagcttc tagtacacta tgagggtcac cttcaagtac agatcgatcc ataaatgcac
    19081 ctgggtcacc ttcatcagca ttacatacaa caaacttttt atctgttgga ccagctggat
    19141 tttttgaagc agcttcccat ttagagccag tagggaaacc tgcaccacct ctaccacgaa
    19201 ggccagaagt ttttacctct gcaataactt cttgaggagt aagagaagtt aaacatttgc
    19261 ctagagctaa ataaccatca ttggcaatat attcttctat actatctggg tctataagac
    19321 cacaattttt taaagctata cgtaactgtt ttttgtagaa agacatttct tcttgttctt
    19381 caactttttt acttaaagat tcttcctcat ataataattc ctcgacaact gtatttctaa
    19441 ttaagtggct ttgaactatc ttttcagcat cagatggctc aacttttacg tagaaaacgt
    19501 tatcaggata aacttttaca ataggacctt gagcacagaa accaaaacaa cctgtcaaac
    19561 gaacactaac tttatcttgg attcctgatt tctttatttc attttctaat tcactaacta
    19621 tttctagact gtttgaagag gtacatccag tatcacaaca aacaagtaat tctcttctta
    19681 atacattttc cttagaataa ttttttctta agctaagatt aggtttcagc tcatcagcta
    19741 aaacttttaa ttcatcaaat gaatttactt tacgcattat ttattcaccc ctcttaacaa
    19801 ttcaattcta attctctata agtatctaat atttcgctta cttggtcagg tttcacttta
    19861 ccataaactt taccatttac agtaacaact ggagctaaac cacatgcccc caaacatctt
    19921 aaaccctcta aagaaaattt aaaatcacta gtagtttctc caacttttat gccaagctgt
    19981 ttttcaaaag cacttaagat tttgtcagca ccttttacaa aacatacagt tcctagacag
    20041 acactgattt tatacttacc tacaggttct gtagtaaaat aagaatagaa tgtaacaacc
    20101 ccatatacct ttgcaggagc tactccaagt tttctagcaa catgaagttg aacttctttt
    20161 ggaagatatc caaatatacc ttgggcttca tgtagaactt gtattaacgc accttccttt
    20221 gttgtaagag agtcaataaa tatatctagt tcgtcaaata attgcttgtt gtgtgaaata
    20281 aaatcgcaca taacaaatcc cccttttctt tttaaagtta ttatttttca taaaataatt
    20341 aaccatgagt tgaccccaac attatgtggg tgaacaaaac cagaacaata aagaaagaat
    20401 aatatacaac aatttcttta tttataaaaa caatgcaaaa aagttaccta ttaaaaataa
    20461 aaactatac
//
LOCUS       DAMJVX010000060        19250 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMJVX010000060 DAMJVX010000000
VERSION     DAMJVX010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19250
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.063"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(3..1196)
                     /locus_tag="PC407_001914"
     CDS             complement(3..1196)
                     /locus_tag="PC407_001914"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDF2497360.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(1219..1884)
                     /locus_tag="PC407_001915"
     CDS             complement(1219..1884)
                     /locus_tag="PC407_001915"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDF2497361.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(1918..3021)
                     /gene="ribD"
                     /locus_tag="PC407_001916"
     CDS             complement(1918..3021)
                     /gene="ribD"
                     /locus_tag="PC407_001916"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDF2497362.1"
                     /translation="MNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNIIGK
                     GYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKRVVI
                     STLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKSAVS
                     LDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQEKVS
                     HPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSKNNL
                     VDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPTFVS
                     GEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(3153..3274)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3463..5946
                     /gene="recQ"
                     /locus_tag="PC407_001917"
     CDS             3463..5946
                     /gene="recQ"
                     /locus_tag="PC407_001917"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDF2497363.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDLKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GANILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVMLDSYLENLKNNIIAE"
     regulatory      6034..6145
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6287..7588
                     /gene="thiC"
                     /locus_tag="PC407_001918"
     CDS             6287..7588
                     /gene="thiC"
                     /locus_tag="PC407_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDF2497364.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            7642..7836
                     /gene="thiS"
                     /locus_tag="PC407_001919"
     CDS             7642..7836
                     /gene="thiS"
                     /locus_tag="PC407_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDF2497365.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            7829..8632
                     /gene="thiF"
                     /locus_tag="PC407_001920"
     CDS             7829..8632
                     /gene="thiF"
                     /locus_tag="PC407_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HDF2497366.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            8695..9465
                     /locus_tag="PC407_001921"
     CDS             8695..9465
                     /locus_tag="PC407_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDF2497367.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            9482..10585
                     /gene="thiH"
                     /locus_tag="PC407_001922"
     CDS             9482..10585
                     /gene="thiH"
                     /locus_tag="PC407_001922"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDF2497368.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYEFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            10586..11275
                     /locus_tag="PC407_001923"
     CDS             10586..11275
                     /locus_tag="PC407_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2497369.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            11463..12857
                     /gene="yfcC"
                     /locus_tag="PC407_001924"
     CDS             11463..12857
                     /gene="yfcC"
                     /locus_tag="PC407_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDF2497370.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            13374..14741
                     /locus_tag="PC407_001925"
     CDS             13374..14741
                     /locus_tag="PC407_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDF2497371.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            14766..15374
                     /locus_tag="PC407_001926"
     CDS             14766..15374
                     /locus_tag="PC407_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDF2497372.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            15422..15793
                     /locus_tag="PC407_001927"
     CDS             15422..15793
                     /locus_tag="PC407_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDF2497373.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            15972..16310
                     /locus_tag="PC407_001928"
     CDS             15972..16310
                     /locus_tag="PC407_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDF2497374.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            16777..17280
                     /locus_tag="PC407_001929"
     CDS             16777..17280
                     /locus_tag="PC407_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HDF2497375.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            17327..18286
                     /gene="moaA"
                     /locus_tag="PC407_001930"
     CDS             17327..18286
                     /gene="moaA"
                     /locus_tag="PC407_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDF2497376.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            18290..18760
                     /gene="moaC"
                     /locus_tag="PC407_001931"
     CDS             18290..18760
                     /gene="moaC"
                     /locus_tag="PC407_001931"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDF2497377.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDYMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            18796..19227
                     /locus_tag="PC407_001932"
     CDS             18796..19227
                     /locus_tag="PC407_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDF2497378.1"
                     /translation="MAKVVSINISEKKGVIKVPVPCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         7512 a         2065 c         3345 g         6328 t
ORIGIN      
        1 atttatataa tatctaaaag atgtcccatt ttatcttttt tagtttttaa atatagctta
       61 ttaaagtcat taatttcttc attaataggg attctatttt cgatttttat tccatatttc
      121 tctaattgat ttattttatc cgggttatta cttaaaagat ttatactttt tatgtttaaa
      181 ttttttaata tttgagcagc catatagaaa tctctcatat cttcttcaaa tccaagcatt
      241 aaatttgctt caacagtatc gtaaccttct tcttgaagtt tataagcctt aattttattt
      301 aaaaggccaa tacctctacc ttcttgcttc atataaatta aaactccact tccattttcc
      361 acaattttct tcatagaaga ttctagctgt aatccacaat cacattttag agagtggaac
      421 acatctcctg ttaaacattc agaatgaagt ctaactagag tattttctat atttatattt
      481 ccataagtaa gggcaatgtg ttcttcattt gaataagtat ctctatatcc tatgatttca
      541 aaatttccat attttgttgg aaggaaagca ctagatactt tttctatagt agtttcattt
      601 attttgcggt attctataag gtcttttata gtgatgattt ttaaattatg cttttctttg
      661 aaaatcatta aatcatcaac tcttgccatt gtaccatcat ctttcattat ttcacagata
      721 agaccaatct ctttaaaacc tgcaagtcta gcaaggtcta ctgaggcttc tgtatgtcca
      781 tccctgacaa gaactccacc agtttttgct attaaaggaa atatgtgacc aggcgttttg
      841 aaatctttac ttgtagaatt ttcatcacag aacaatttta cagtttcagc tctttcatat
      901 gcagatatac cagttagagt atctgctcca tctatagtaa cagtaaaggc tgtttgaaat
      961 gtatctgtat tttctctaac cataagaggt atattaagac ttttaaattt tttttcagtg
     1021 gcaggtagac aaattagacc tctaccatat gtagccataa aatttattga ttcaggagta
     1081 gccatttcag ctgccatcaa taaatctcct tcattctctc tactttcatc atcaactacg
     1141 attaccattt tcccattttt tatatcttca atagcttctt caatagtatt aaacatgtaa
     1201 ttcactcctt tattattttt aaaaaccatt tttaaataaa aaatctttat ctatatttga
     1261 tttatggtta ctactattag aatttaattc cttgtagtta ttagccataa aattattaac
     1321 atactttcca attacatcgc tttctaaatt aacaaaatct ccaacattct ttgtcaagag
     1381 aatagtgtta attttagtat gaggtataat agaaacctta aatatttcat catctaaata
     1441 tgatatagtt aaacttacac catcaatagc aacagaacct ttgagtatca ttgaagataa
     1501 aagtttttta tcaggtcgta tacttactaa aactgcattt tcatcttttt caaatttgca
     1561 gatagttcct ttaccatcaa catgacctgt tactatatga ccaccaaatc ttgaagaaag
     1621 actcatagct ctttcaagat ttaccttatc atttgcattt aataaaccta aatttgtgga
     1681 acgaattgtc tccatcataa catcggctgt aaattcattt tttccgaggt ttgaaacagt
     1741 tagacaaact ccatttacgg ctatactatc tccaagattt gtgccatcta atattttatt
     1801 tgatagtatg gttacttttc cactcttacc attagcagta atttttctaa gtattccaac
     1861 ttcttctact atgccagtaa acatcctaac ctcctatatg aatgtattaa caaaaattta
     1921 atttaaaaca tctgattcta taagaatatc gccatctatc aaagttacat tattgattgt
     1981 taactgtgtt gaatctgaca aatgatttat tccttctcca gaaacgaatg taggtgcatc
     2041 tttaccacct ataatcttgg gtgatagaaa taatttgact ttatcaacta acttatttct
     2101 aaagagactg tcattgagag tagcaccacc ttcaactaga acagaatcaa tgtttaactc
     2161 tcctaattta tacattacaa attccaaatc tactaagttg ttttttgaag gagaaattat
     2221 tgtttcaaca ttattttctt ttaacataga taattttata ctttctttgc aacatgtgaa
     2281 aactatagtt ttactggttt tatctttaac caaattacta ttaagtggca actttaggtg
     2341 tgtatctatt actattcttg taggatgaga cactttttct tgattgagcc tgcaagttag
     2401 taaaggatta tcgttcaaaa ctgtatttat gccaaccatt atactttggt atttatttct
     2461 gtatttatga gttagatacc ttgatgattc attagatatc cattttgatt ctagtgattt
     2521 tgtagcaatt ttaccatcta gacttactgc tgattttaca atacaaagag gtcttttgtt
     2581 ttttatatga taaaaaaatg cctcatttaa atctcttgct tcatcttcta atattcccac
     2641 agtaacatca atattgttat cttttaattt ttttactcca ttaccacaaa caagtggatt
     2701 tgggtctaat gtagatatta caactctctt aatcttattt tgtataattt tatctacaca
     2761 aggaggcgtt ttcccataat gagaacatgg ttctaagttt acataaagag tagagtcttt
     2821 tagactttgt ttagcactgt ttatagcatt tacttcagca tgattagagc caaatttctc
     2881 atgatatcct ttgcctatta tattactatc tttaacaatt acacacccaa ctaatggatt
     2941 tggatttaca aagccttctc catttttagc caattcaata gcttttttca tataataaat
     3001 atctttttct ttttgattca caatatgtac acctcctgtt taattggata atttttaaac
     3061 aaaaaaccct gagaattaac tcagggttta gaataatcta caataaatgt ttaataaaag
     3121 tagaagaaaa ttgctctaca aaaacactca ttaaatatct tcttccatcc agactttact
     3181 gtcggctttg gaatttaacc aaatcaacca agttttacct tggctcgcgg gctataccgc
     3241 cgattgggaa tttcacccta ccccgaagat taagtattaa atttatattt agaatgtatc
     3301 ataaattatt ttactttgca aactattttt ttaaaaaata tatgttaata tataatatag
     3361 aatgtctata aactttgaaa taccacgatt gtgaatataa aaataaaaat ttgaacataa
     3421 tcaggaaaga agtctaatta atttaaatta ggagagtaat atatgaataa aaaactttta
     3481 gaaatactat caaaatacta tggatataca agttttagaa aaggtcagga atctataata
     3541 aattctatct taagcgaaaa agatgtgtta gctattatgc caacaggagg ggggaaatca
     3601 atatgttatc aaattccagc tttgatttta gatggaatga caatagtaat ttctccactt
     3661 atatcactta tgaaagacca agtagatgcc ttaaagacta tgggaattag tgctgctttt
     3721 ataaatagtt cattaagcag caaagaattt aatgaaatat taaataatat tagaaaaaat
     3781 aattataaaa tactctatat tgctccagag agattagatg cacaagagtt tattgagcta
     3841 ataaataata acaatgtaag tcaagttgcg atagatgaag cacattgtgt atctcaatgg
     3901 gggcatgatt ttaggttgag ttatagaagg atttctgatt ttataaaaaa tttgcctaaa
     3961 agacctatag ttacagcatt tactgcaaca gcttcagaag aggtaagaac tgatataata
     4021 aatttactgt gtttagaaaa tccagattat tatataactg gatttgatag agagaattta
     4081 tcaataaata tagtaaaatc atctagtaaa aataagtata ttttagatta tattcaaaat
     4141 cataaaaatg agtctggaat tatatatgtt gcaactagaa aggaagttga aaatatatat
     4201 aatggtttat ctaagagaaa tatatctgta tcaaaatatc atgcaggttt atctaaaaat
     4261 gaaagaagta aaaatcaaga agattttata aatgataatg tggatataat ggtagcaaca
     4321 aatgcatttg gaatgggtat tgataaacct aatataagat gggttataca ttacaatatg
     4381 cctcaaagta tagaaaacta ctatcaagaa ataggtagag ctggtaggga tggagaaaaa
     4441 agtgagtgtg tacttttatt ttctccagga gatgtacata tacaaaaata tttaattgat
     4501 attggagttg aaaatcctga aagaaaatta tttcagcata agaagcttca atacatgatt
     4561 gacttagttt acagtaattc atgctataga aagacaatat tgagttattt tggagaaaat
     4621 tatatagaca attgcaataa ttgtagcaat tgtttagtag agggtgaaat agtagataaa
     4681 actatagatg cacaaaaagt catttcatgt gtagctagaa tgaaaagaag ttatggtgtt
     4741 actactattg tagatgtact tagaggttct aagaataaaa agattttaca gcttggatta
     4801 aatactttgt ctacatataa cattatgaga gattattcta gtgaagactt aaagaatttt
     4861 gtaaatacat tggtttctca tggtttttta gatttagttg agactctagg caatggaaat
     4921 agaggtagtt ttcctactat aagattaaat gagatgtcta tgaaagtttt aaaaggtgaa
     4981 gtgaaagtag agtttaaaga aattgttatg acaaaatcat tggaagtaga ggatgagtta
     5041 tataatgctt taagagaact tagacattca attgcaagtg aagaaaggat agctccatat
     5101 atggtatttg gagatggtac acttagagcc atgtctagta gttatccaat taataaagaa
     5161 gaaatgctag atatctctgg agtaggagaa attaaatatc aaaaatatgg tagagatttt
     5221 gaagcagtaa ttaaagaata tgtcgaaaaa aataatataa ataaaaaaat agatgaaaaa
     5281 aatgtcgatt taaagaacgt taatagtgat ttcttagagg ttaatacaga taaagtttta
     5341 tatgaaaaac tgttagtcat tagagaagaa tatgcaacaa aggaaaaatt acctgcatat
     5401 atgattttag ccaataaggc tttgaaagaa ataagtggaa gatatccact tgatgaagaa
     5461 caattaaaag atatttctgg cataggtgga gtaaaatttg aaaaatatgg agctaatatt
     5521 ttggcagagg ttaataaata tgtaaaagaa aataatatta atgttatttg ggagaaaaag
     5581 ggcaaaagga agttaatatt agatggagaa agtagaaaaa ataatgaaat agcattagat
     5641 ttgctaaatc aaggaaaaga tatacaaagt atcagtcaag aaatagaagt ttcattgtca
     5701 accattattg gatatgtgca tgactatgta aaggatggac atagtatatc atttgaatta
     5761 ggactagaaa atttttataa tgaagataaa aagaagatga ttttagaagt ttgcgaaaaa
     5821 gttggctatg ataatttaaa taacataaaa agaaaacttc cagattcaat aaagtatgaa
     5881 aatataagag ctgtgatgct tgactcttat cttgaaaact taaaaaataa tattatagca
     5941 gagtaatttt taacttaata aaaataaaat ttgtattatt gtgttgaact aataattttg
     6001 ctgtgttata ctgaatttgt aaaaataatg aaaaattaga gctaggggcg ctagaattgg
     6061 ctagctgaga gataaagccg taacttacaa accctaataa cctgatcaag ataatgcttg
     6121 cgaagggaag ctttttaata taattttatt atataaatag gcaaattatt caatgttagg
     6181 agaaatttgt ctaagtgtat atacgattat aagagtgctt tccaaacgag gaaaagcact
     6241 ttttttattt tcaaaaacta taactttaat cttggaggat tgtaatatga actatacaac
     6301 acaaatggat gctgctagaa aaggcataat aactaaagaa atggaaatcg tttctcaaaa
     6361 agaacaggta gatgtaaatg aattaagaga attaatagca aatggacaag ttgttatacc
     6421 agctaataaa aatcataaat ctttaagtgc agaaggtgta ggtaaaaatt taagaacaaa
     6481 aataaatgta aatctaggaa tttcaagaga ttgtaaggat atagaaaaag aacttgaaaa
     6541 agttagagta gcaatagaca tgaaagctga agctataatg gatttaagta attatggcaa
     6601 aactagagaa tttagagaaa aggttgttga aatgtcacct gcaatgatag gttcagtacc
     6661 tatgtatgat gcagtaggat atttggaaaa agaattaaaa gatataacag aagaagagtt
     6721 tttaaatgta ataagacaac atgctattga tggagtagac tttattacta tacatgcagg
     6781 tcttacaaga agtgtttgcc aaaaaataaa aaatcatgag agattgactc atatagtatc
     6841 tcgtggaggt tctttattgt ttgcatggat ggaattaaat aataaggaaa atcctattta
     6901 tactaatttt gataaaatat tggatatatg tgaagaatat gatgttacat tgagtttagg
     6961 agatgcttgt agaccaggtt gtataaaaga ttctactgat ggtgttcaaa ttcaagaact
     7021 tgtagtatta ggtgaactta ctaaaagagc atgggaaaga aatgtacaag taatgataga
     7081 aggtcctgga cacatggcta ttgatgaaat agaagcaaat gtagtattag aaaagagatt
     7141 atgccatgga gcaccttttt atgttcttgg accattggtt acagatatag caccaggtta
     7201 tgaccatata acaagtgcaa taggaggagc tttagcatgt gctaaaggag tagatttttt
     7261 atgttatgta actccagcag aacatttaag attgcctaat ttagatgata tgaaagaggg
     7321 tataatagct gcaaagatag ctgctcatgc aggagatata gctaaaaatg ttaaaggtgc
     7381 tcgtgaatgg gataataaga tgagtaaagc tagagctgat ttagactggt gtgagatgtt
     7441 tagacttgca atagaccctg aaaaagctaa gagatacaga gatgaatcaa ctcctactca
     7501 tgaggatagt tgtactatgt gtggaaaaat gtgctcaatg agaacagtta agaaaatatt
     7561 aaacaatgag gaacttaatc taatataatt tttggctagt atgactggaa taaaaagaag
     7621 ctgaaaagag gaggtgattt agtgaaggtt aatgggaagg aaattgaatt tgaaaaagat
     7681 ttgactgtta tagatttatt aaataaatat aatttaaaaa gtgatagagt tgtagttgaa
     7741 gtgaatttag agataattga agaatctaac tacaatacat atgtattaaa agacgaggac
     7801 attgtagagc ttattagctt tattggaggt ggttaaaata aaaatatttg taaatgaatt
     7861 attaacagat gttgaggaaa atacaactgc ttataaagtt agagatagct ataacaataa
     7921 atgtgatgta ttggtgttaa atggatatcc aattaaaaca gatatgcccc tttgtgaaaa
     7981 tgataaatta acacttatac aaaaaggagt taagccatca cttgatgaat tggaaagctt
     8041 gttgattgct agacatactc caaatataca taataaatta aaaaaaggga aagttgctat
     8101 attgggattg gggggacttg gttccaatat agctatctct ttagctagaa taggagtggg
     8161 agaactttta cttatagatt atgatgttgt agaaccatct aatttaaata gacaacagta
     8221 ttttatagat gatataggaa aactaaaaac tgatgccatg atagaaaata taaaaaaaat
     8281 aaatccattt ataaaattaa ataaacgaga tattttttta aacaagaaca atatggatac
     8341 tattaaggat tcagatttaa taattgaggc ctttgatgat gcttcatgta aagcacaggt
     8401 atgtaattat gtattaataa atttaaaaga taaatattta atagcatcat ctggtatggc
     8461 aggttactat gattcaaata taataactac taagaagata aaagacaggt tttatatatg
     8521 tggggatttt gtgaatgaag ctaaatttgg tgaagggctt atggctccaa gggttgccat
     8581 ctgcgcaaat catatggcaa atttggcaac gcaaatactt atagatatgt aaatattaaa
     8641 attagatata aaaaagattg aatattaaaa aaatttaacg tggagggaaa taatatggat
     8701 aaattggtac ttggtggaca tgaatttaat agcagattat tggttggaac aggtaaatat
     8761 ggttcaaata atatattgcc tgaggtaata aaagaaagtg gaagtgaaat aataacaatg
     8821 gcacttagaa gagtggattt agacaataaa caagaaaaca tattaacgta tattccaaaa
     8881 gagatgacaa tacttccaaa tacatcagga gctactaatg cagaagaagc tgtaagaata
     8941 gctagaatat ctagaaaaat gggatgtgga gattttataa aaatagaagt aatatcagat
     9001 actagatatt tacttcctga taatgaagaa actataaaag ctacaaagat acttgctgat
     9061 gaggggttta tagttcttcc atatatgaca ccagaccttt atgctggaag aagacttata
     9121 gaagctaatg cagccgcagt tatgccactt ggagctccaa ttggttcaaa tagaggtctt
     9181 caaatgaaag aaatgataag aataatgata gatgagttgg atataccaat aatagtagat
     9241 gcaggtattg gaaaaccatc tcaagctatg gaggctatgg aaatgggagc tgatgcagta
     9301 cttgtcaaca ctgctatagc ttcagcagga gaccctgtac aaatggctag agcatttaaa
     9361 ttagctgtag aaggtggaag agaagcatat attgcaaaaa caggaaatgt gtcagaattt
     9421 gcaaatgcat cttctccttt aacaggtttt ttaggcaatt tatagaaagt ggtgattggt
     9481 gatgagtttt tatgatgtaa tagaaaaata tagggatttt gattttgatg gatatttaaa
     9541 taatgttact gataatgatg ttttaagaag tttatcaaaa gacaagcttg aagattttga
     9601 tatattaaat ttgctttcta aaactgcagt aaaacattta gaagatatgg cacaaaaagc
     9661 acataaatta agtgttcaat actttggtaa aacagtatgt ttatatacac caatgtatat
     9721 agcaaattat tgtgttaatc aatgtgttta ttgtagctat aatataaaga gtggaataaa
     9781 aagaaaaaaa ttaactatgg atgaaattag agaagaggga gaagcaattt caaaagaagg
     9841 tttcaaacat ctacttgttt tgactggaga aagtagtttt cactcaagtg tagaatatat
     9901 aggtgaggct atagaaatat taagagagaa atttccctct ataggtatag aagtttatcc
     9961 aatggaagta gaagaatata agtacattgt agataaaggt gttgaaggat taacagtata
    10021 tcaagaaact tatgacgagg aaatatataa gagagtacat ataaaaggcc caaaatcaaa
    10081 ttatgaattt agactagatg ctcctgaaag aggggcaaaa gcaggtatga ggactttatc
    10141 aataggtgca cttttaggtc taaatgattt tagaaaagaa acttttttta caatattgca
    10201 tgggaaatac ttaaaaacga aatatcctca tattgagctt tcatattcaa caccaagaat
    10261 gagacctttt aaagggtgtt ttgaggaatt ggtagatata agtgatactg atttagttca
    10321 agctatggta tgtatgagat tatttgaccc tcatgcagct ataaatatat ccactagaga
    10381 aaatttagaa atgagaagtc atataatacc tttaggagta actaaattga gcgcaggtgt
    10441 atctactgat gtaggaggtc attctcaaga tgaacatgat actgctcaat ttaagataaa
    10501 tgatgaaagt actgttaaag atgtggagaa aatgctaaac tcaattggtt atcagcatgt
    10561 gtttaaagat tgggaaagat tttaaatgta tctgataaca aacagaaaat tatgtagtga
    10621 ggaacgatat ttagaagtta ttaaagagtc tatattgagt ggtgtagaaa atataataat
    10681 aagagaaaag gatttggaat atcaagagct aagaaaatta tatatgaaaa taaaaactaa
    10741 aatcaattgt atagattttc aagagcaaat atcagatgaa agtttgaaaa ctaatataaa
    10801 tcaaaaagag tgtagaaata aattcaaggt taattttata ataaatagta acattgaatt
    10861 ttttgaaaaa gtggattgtc aaggtatcca tcttcctttt aaattattct tgaatttaat
    10921 tgaaaataag tataatttta atgaaaataa aatactagga ttatctttac ataaagtaga
    10981 ggaagtagat tatttagaaa aattaataag aaatcaaaat ataaaaatag attacataac
    11041 attatcacat atatatgaaa ctaaatgtaa agaaggatta aatccaaaag gaattgagct
    11101 tttaaaagaa gctaaaaaga ttactgatat taaaataatt gcacttgggg gaattttacc
    11161 atctaatgtc aaagaaacat taaaatactg cgatgatttt gctataatgt caactataat
    11221 gaggtctaaa gatattaaaa aaaccatatc aaattataat gaaaagttga attagtaaat
    11281 atagtatatt gaatttttaa cttaaataaa aataaaaaag ttatctttta aattttaaaa
    11341 ggtagctttt ttattttata tctttgctaa taaaaaattg taaaaaggga aaactttagt
    11401 cagagagatt aaacaatatc ctagaaagat ttaaaattta gtaaagtatg gaggacatta
    11461 taatgactga cagtaaaaag aaacgattta ttataccaca cacttttaca ataatatttt
    11521 tactaatagt cttgatggca atattgactt ggatagtacc aagtggtgaa tttagtagag
    11581 aagatgtaga aggtagaatg gtagttgtac cagggactta tcaaagtgta gaaagtaatc
    11641 ctcaaggatt tgcagatgtt tttaaagcac caatagaagg ctttatagat tcagctgaag
    11701 ttgtaggttt tgttttaata gttggaggtg cttttggtat tgtaaatagg actggagcta
    11761 tagaagcagg aatagcttcg gttatctcta agtttaaggg aaaagaactt cttataattc
    11821 caatatcaat gattttattt ggacttggag gtacaacatt tgggatggca gaagagacat
    11881 taccatttta tatgatattt gttccactca tgacatcctt gggatatgat tctctaacag
    11941 caatagcaat tgtatttata ggagcagcag caggagccgc cgcttcaaca ataaatccat
    12001 tttcagttgg tattgcacag gcactagctc aacttgctcc tggttcagga gtaacttaca
    12061 gagtagttat ttttatagca tatatactaa taagtatagc atttgttatg tactatgcta
    12121 agaaggtaaa ggaaaatcct gaaagttcaa tagtatatga aatagataaa aataataaat
    12181 caatacttaa tcaaacttca acagaaataa aagaatttac ttttaaagaa gtatctgttc
    12241 ttgctatatt tggaataggg atggcaataa tgatttgggg tgttttaagt caaggatggt
    12301 acattccaga aatatcaatg tgttttgctg caataggtgt gctttctgga ttggttggta
    12361 gattatctca aattgaaatc tcagaaagtt ttatagatgg agcaaaagat ttagcatctg
    12421 cagctcttgc tatagctttg gctcgtggaa tcgtaataat tgctcaaaat ggatttataa
    12481 tagatactat attaaattcc gcagcagcct ttctaggtgg gcttccaaag gttatatttg
    12541 tatatttatc atttttcttg gaagctgcat tggcattttt aattccatca tcttcaggtc
    12601 ttgcttcact tacagtacct gtacttgctc ctcttggtga tttggttaat atatcggctc
    12661 aagtgatagt aaccgcttat caatttggtg tgggtcttac aaactttata actccaactt
    12721 caggagtact gatgggagca cttgcagtag cccatattcc attttcaaaa tgggtaagat
    12781 ttataatgcc tatcttgttg attttgacag ctgtatctct agtcttttta acaataggta
    12841 tttatatagg gttttaatta agtgtagttt tatttaagat atttagtaat ggtataaaat
    12901 gataatatgt tttcaataaa ttgccactaa tttaattagt ggtaatttat tttttgtatt
    12961 taaaaataaa tgtatttact tgtattttga attaatatct acttagacat tgttttaatt
    13021 gttatatttt catttaatta agcattgatt tatcgttttt tatatttcat ataacaatgc
    13081 aaaaaataat ttttatatag taaatgacaa aagtttacaa taaatgtata ttttcttact
    13141 cattccttaa aattactcat ttagtttaat aactctcatt tcatatgtaa agttggtaaa
    13201 aaaattaaaa ttaaaagtaa aaaactaaat aaattaatgt taatttgaaa tatatatgaa
    13261 ggtttttgga gaatacttac ttatgatagg aaaaaatgtt aaaataatag tgtaatcaaa
    13321 tataaaggac tgaaataatt aacagttatt aaatattagg agtgtaaaaa tttatgggat
    13381 taaaagaatt tgattttatt gaagagtcta ttgatatgtt gaggacaatg tcaccaacat
    13441 tagaaactat atcagatgaa atagaagagt attttgaaaa tatcttggat gaaaagaatc
    13501 aagaatatat aaatgtaacc tctagaataa aatcagaatc aagtttaaga gaaaagataa
    13561 ttagaaatag atatttaaaa aaatatggag aagcaagtaa tcttattcat aatgtttctg
    13621 acttaatagg cctaagaatt gagtgtagat ttattgagga tgaaaacaaa atatatagac
    13681 ttttgagaag atattttaat aaaacagatg acaaaataaa ctattataat aaagagaata
    13741 agaatattaa attaaaacta tctgagaggc aacccaataa gcaaaaaaat ggatttgaaa
    13801 tatataaaat agatggagtt tttgcgtatt tagatagaga agtaaaattt gaacttcaaa
    13861 ttaaatctct tgtaaatgtg ttttggagtg aaatagaaca taaaataatt tataaaaata
    13921 atacatattt acttgctgat aaatttataa aggatatgat ggactctata aagaataatc
    13981 ttactatgat agataatcag cttttaagta tttataaaaa ctttcattca gggaagtctt
    14041 ttaatatgaa agtaagtaaa aaagaaattg aaaaattatt tgcgaaacta gtctatgacg
    14101 ctttttctga aaagatgaat aaaagtatag gttttgttgt agattttaaa aaaccatgtg
    14161 aaactatatt gaactattct tttaataaac aagatataag tgatgaagtt ttaggtaatt
    14221 ttatgcttga tgagtttgca agattaaatg aaattgtaaa caaggatata gattttaatg
    14281 agcaaataga atttgaaaga gaaccaacat ttgatgataa gttttgtaaa gacctaggaa
    14341 atcattttag aagtagatta aacacagaat ttccatggaa cctatttttt agaatattat
    14401 ttgaaataga gccatataac aataccaaag actttgaaaa ctttgtagag tttataaaag
    14461 aaaatgtgct actagaagaa aatagggagc aattattatg ccaatttgaa gaagatagca
    14521 agcttataat tgaagatata tatgattgta tatttaatag tataagtgag attgatagtg
    14581 ttgaaatttt atataactat aatcttgaaa aaataaactt tacatcaagt gaaatcataa
    14641 attgtatatg tagagaatat gagtgttatg gtgaatactt agaagaaaaa gaaaagctta
    14701 tgaatacatt taaagaaaag attattcaaa tctttgagta ataggtcaat gctgaagtgg
    14761 cgctaatgcg ccatttttca aatatatatg aattaagtaa aattattttg gaggaggcga
    14821 tttgtataga gaaaacaggt gtaattttgg cagggggaaa aaattctaga atgggtagag
    14881 ataaagcctt tttagagtta catgataagc tttttattga aattgcaata gaagcattta
    14941 aaaactttga tgagttgata ataatatcta ataatgaaga attatattct aaatacgata
    15001 ttaaagttta taatgatata gtaaaagatg ttggtccaat tggaggaata tatactgctc
    15061 ttgaatatgc aaagtatgat atagttacaa ttgcatgtga tatgccatat ctaaataatc
    15121 aaatagtcga aagaatagct aataaaatga atgataaaag tgtgatttca gtgacaaatg
    15181 gaaagttaca accattgtgt tctggatata aaaagagtat aataaataaa gtatctttat
    15241 gtataaggga aaatgattta aagctcagaa gctttataga taaaatagat aaaagttata
    15301 tatactttga tgaagaagat ttatttttaa atgtaaacac tgttgatgag tatgaaaaat
    15361 taactaaagt ttaattagct atggttgtaa aatagagtag gatttatatg gaggagagtt
    15421 tatgaattta cttaaatgcg attcttgggc agtaatatta aataatagcg ataaagaaag
    15481 taaagcatat aaaatattag atgaacttaa aaggaatatg tacaaagttg ttgctataga
    15541 tgaagagaaa aaacctattg aaggaataga tgtgtatgaa tgtttaaaag atgttccaca
    15601 caatattgat gttgtagcta ttattgataa acaatctaag atggatgtga ttttagaaga
    15661 agtagagctt ttggatatac aaaatatgtg gtttgaaaaa ggaagcttta gtgaaatgat
    15721 gattaaaaag actaaagatt taaaattaaa tatagaatat aatttaagtt tatatgatga
    15781 gttgactaga taaaattata tttcttaata ataaaaatat aattagttaa taaaacatat
    15841 tattttatag tatagattgg ttaataaaag tacagattgg ttaatgaaac attaaattac
    15901 aatatttgtt aaaagaattg tgagttacat gatataatgt attaaatagg atactaagga
    15961 gttgatatat tatgttaata gtaactacag aaaaagtaga aggtaaaaag atatcaaagg
    16021 ttttaggatt agtgagagga agtacaataa gagcaaaaca tgttggaaaa gatataggag
    16081 caagttttaa gaatcttgtt ggaggagaac ttactggata taatgaaatg ctcactgaag
    16141 caagacaaat tgccataggt agaatggttg aagatgcaga agctaaaggt gcaaacgcag
    16201 taatagcatt tagactgtct tcagcttcag ttatgcaagg ggcagcagaa atgcttgctt
    16261 atggaacagc agttgtttta gaagatgata atagtattct tgaaaaataa aatataaata
    16321 gttaaaaaga ggcatctttt aaaaatttta tggatgcctt tttttatttg aaaaaactaa
    16381 tttgagaaga aattttaact attatgacaa taaaattagg gtgatattat ataaataagt
    16441 aaaattatat tatataatgt atggtataga ctttaaaaat tttatttgta gagaaaaata
    16501 tggacaatgt tataataatt atagaaatta aaagtactta cattcattat gtatgtgaca
    16561 tgtataatta gttttatatt taatacagtt ttgaagtaat atcttcattt tgagaacgtt
    16621 ggaataataa aaaatgtatg tattataggt gatataaata aaaaataaat ataatatgtt
    16681 atattgctga tagaaaagag aataaaagaa aataggtttt attaaaagag atttttattt
    16741 ataggactta atcaatgtat agaggggtga gaacatatgt ttaatgttgc aattataaca
    16801 cttagtgata aaggatatga aggaaaaaga gaagatataa caggaaaaaa gttaactgaa
    16861 tttgttgaaa atacaggagc ttataaagtt actgagtatg tacttataaa agatgataag
    16921 gaaatgctaa aggaaaatat tataagactt tgtaatagta ataaaataga tttaattctt
    16981 acaaatggag gtactggttt ttcaaaaaga gatataaccc cagaagcaac taaggaagtg
    17041 ttggaaaaag aaataccagg tctgagcgaa tatatgagaa tgaaatccac agaaattact
    17101 aaaaaggcca tactaagtag gggtgtgagt ggaataagaa ataattctat aataataaat
    17161 ttacctggaa gtcctaaagg tgcagttgaa aatctaagtt ttataattga tgttttagac
    17221 catggcatag aagttttaag aggagaagct acagaatgtg ccactaaaaa aagtgaatag
    17281 tatataatct agatagataa aaaatagaca aagaggtaga taaagtatga ttgataagta
    17341 tggaagaaaa atagactacc ttcgtatttc acttacagat aagtgtaatt taaggtgtgt
    17401 ctattgtatg ccaccagatg taaaatttga taaaaattat ataaatgaaa atctaagctt
    17461 tgaagactac aagtttatta taaaggctat ggctgaacaa ggtataacaa aaattagatt
    17521 tactggagga gaacctcttt tatatcctaa attaaatgag cttataaaat ttacaaaaga
    17581 agaatgtgga ataaataaca taggaatgac tactaatgct attgggcttt ctgatagaat
    17641 ttttgagctt gaaaaatgtg gcttaaataa agttaatata agtttagatt ctttgaagga
    17701 atataagtat aaatccgtaa caagaggagg aaacttaaaa gatgtcttaa aatcgattga
    17761 atcttgttta aacttaggta taaaagtaaa gttaaattgc gttgctataa gtgggtttaa
    17821 tgatgatgag cttaaagatt ttatgttttt aacagttaaa tcaccaattg atgtaagatt
    17881 tatagagctt atgccattag gtgaggctaa tagagtttat aagaagggtt attttaatgt
    17941 aaaagaaatg attgaaaaaa tatatggttt atataaagtc aaaaatgatg aaaaaagtac
    18001 agcagaatat tatatgttta aaggagctaa aggaagaata ggaattataa ctccattaag
    18061 ctgttcattt tgtaacactt gtaatcgtat tagacttact tctagtggtg caataaaatt
    18121 atgtctacat tcaaaagaag aaatagatat aaaaccattt ttacataaac cattgttatt
    18181 tagagaatct atgaaagata taataaaaca aaaaccagaa aaacatcatt taatagaaaa
    18241 taaatgtagt gatacaaata ggcgtatgta tgaaattgga ggataagtaa tgttaacaca
    18301 tataaatgaa aaagggtatg ccaagatggt ggacataagt gaaaaagatg atactaaaag
    18361 aacggccgta gctacagcta caattactct tagtaaggaa gcattagaaa aagtaaaaaa
    18421 tggtcagata aaaaaaggag acgtattgtc tgtagctcaa gttgcaggta taatgggagc
    18481 taaaaataca tcttcaaaca taccaatgtg ccatcagatt aaattagttg gatgtgattt
    18541 agaatttgat gtagatgatt atatgtctgc tattaagata tacgcaacct gtaaatctct
    18601 tgggaaaact ggagtagaaa tggaagcttt aaatgcatgt tctttagcag cacttacaat
    18661 ttatgatatg tgtaaagcag ttgataaaaa aatggttata agtaatatcc atcttgtgga
    18721 gaagactggt ggaaaatcag gagattttaa gttcgagtaa attaataata tattgggtta
    18781 aaatttggag gaaatatggc gaaggtagtg tcaataaata taagtgagaa aaagggtgta
    18841 attaaagttc ctgtaccatg tgcagagcta aaaataaatc atgggatagt tggagatgca
    18901 catgcaggaa actggcatcg tcagataagc ttacttggaa aagaaagcat agataaaatg
    18961 aaaatgaaag gctttgacca attaaaatat ggagattttg ctgaaaatgt aactacagaa
    19021 gggatagaag ttttttccct gcctgttggt acaaagttaa gaatagggca atgtgaagtt
    19081 gaagtaactc aaatagggaa aaaatgtcac aatggatgtg aaattaagaa attgacaggt
    19141 gactgtgtga tgccacgaga aggaattttt gtaaaggttc taaaagaagg aattataaat
    19201 gtgaatgatt caatagatgt aatctaatga agtcactatt aaaatataat
//
LOCUS       DAMJVX010000061        19231 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMJVX010000061 DAMJVX010000000
VERSION     DAMJVX010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19231
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.064"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            35..373
                     /locus_tag="PC407_001933"
     CDS             35..373
                     /locus_tag="PC407_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HDF2497379.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(500..1789)
                     /locus_tag="PC407_001934"
     CDS             complement(500..1789)
                     /locus_tag="PC407_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2497380.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFFTGNNILMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            1869..2573
                     /locus_tag="PC407_001935"
     CDS             1869..2573
                     /locus_tag="PC407_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HDF2497381.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            2665..3711
                     /gene="selD"
                     /locus_tag="PC407_001936"
     CDS             2665..3711
                     /gene="selD"
                     /locus_tag="PC407_001936"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2713..2715,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDF2497382.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDNPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            3729..5141
                     /locus_tag="PC407_001937"
     CDS             3729..5141
                     /locus_tag="PC407_001937"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDF2497383.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVAELRKAFN"
     gene            5255..7138
                     /gene="selB"
                     /locus_tag="PC407_001938"
     CDS             5255..7138
                     /gene="selB"
                     /locus_tag="PC407_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDF2497384.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALEALISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            7243..9981
                     /locus_tag="PC407_001939"
     CDS             7243..9981
                     /locus_tag="PC407_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HDF2497385.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSIILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(10100..11044)
                     /gene="manA"
                     /locus_tag="PC407_001940"
     CDS             complement(10100..11044)
                     /gene="manA"
                     /locus_tag="PC407_001940"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDF2497386.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            11217..12578
                     /locus_tag="PC407_001941"
     CDS             11217..12578
                     /locus_tag="PC407_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2497387.1"
                     /translation="MELFKAESKKYEKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSIVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            12666..14555
                     /locus_tag="PC407_001942"
     CDS             12666..14555
                     /locus_tag="PC407_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2497388.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIYCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            14570..15025
                     /locus_tag="PC407_001943"
     CDS             14570..15025
                     /locus_tag="PC407_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2497389.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            15084..15401
                     /locus_tag="PC407_001944"
     CDS             15084..15401
                     /locus_tag="PC407_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2497390.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            15426..16688
                     /locus_tag="PC407_001945"
     CDS             15426..16688
                     /locus_tag="PC407_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2497391.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFIDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            16793..17857
                     /locus_tag="PC407_001946"
     CDS             16793..17857
                     /locus_tag="PC407_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2497392.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKIGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKIIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            17911..19089
                     /locus_tag="PC407_001947"
     CDS             17911..19089
                     /locus_tag="PC407_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDF2497393.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKNIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
BASE COUNT         7742 a         1996 c         3353 g         6140 t
ORIGIN      
        1 gaaaatcaat atcaaataaa tgggaggaaa tctaatgaac ttattagtga ttggtggtca
       61 tgaaagaatg gaaaaagatt atatggttat ggccaagaaa aaaggttata agacaaaggt
      121 atatactacc atgtcatcta aattaaataa ttctatagga agaccagatg cagttgtcat
      181 acttacatca actgtttcac ataagatgtc tagaacggtt gagtctcaag ctaaaaaaca
      241 agatatatta atcgtaaggc ataaaaatag tagtaaggta gcatttaatg aatgtctaga
      301 tatggttgat gaatgtctgg gaaattgttc aaaatgtaag cttaaagaca aacaaaaaca
      361 aaaaaatata taggtataaa aaatacataa acataaaatt aaaagtactg agataatagt
      421 tataattatt atctcagtac ttttagattt ataaggataa gtatattata aattttttat
      481 tataatttcc aactacttgt tactttttca tatttttttt acttctagct ttttttcttt
      541 ttttcttctt tatatttgaa gctattataa ataaaactaa aagcactaga atacctgcaa
      601 taatcttaac aaaagtcatt aaaatattat ttccagtaaa aaatggaaga ctgctatcaa
      661 gattattttt tgcaattaag tctatactgt tttctagttt accattatta tatacattta
      721 aagtaccaac cttagctcct ttttttatag gcaaatcaat tttatttaac tctgttttgg
      781 tatcataatt gccttgagat tcatcttttt ctaagaatat cctataacta ttttttggtt
      841 cataaatcaa ttcatgttgt tttgtaaaca atactttttt agtatctgtg tatttttctt
      901 tatcaacaat agtagcacac ttaaagttat caaatccata atcaatcaga atacgtgagt
      961 ctaaatataa atcagcatta gttgaattaa atacagcaac gataactctt ctgccatctt
     1021 ttacagcact cgataaaaga catttaccag cagcatcagt atatccagtt tttataccat
     1081 caactatatc atattttata tttataggtt gacctttgta tgtaatctta tcatgtgatg
     1141 tcaaaaactt attagtgttt acaaagtatc tctcataagg atatgctttt gtagcttcaa
     1201 atttaagtga cttagttttt acaatttgtc taaaagtatc attattcata gcttctctag
     1261 ctatgagagc catatcatga gctgttgtgt agtgatttgg gtctggtaat ccatgaggat
     1321 tattaaaatg agtattagta gctccaattt tttttgcctc agagttcatg aggtcaataa
     1381 atttttgtac attgccacca ccaacatatc tagccaatac aaaagcagca tcattagcag
     1441 aatgaaccaa aagagcatct agtaactgct ttacagtaaa agattcccca ttttttagat
     1501 acatactaga gccatctatt tgaggtaaat ctttaatttc tattacttga tttaaatctt
     1561 taacctcctt tagaactaaa caagctgtcc atactttagt tgttgaagct ggataaagct
     1621 tagaatttcc atccttatta tatagtattt tgccagactc atagtccatt aaaactgcat
     1681 gtttagcaac tatagctggt tcattatctg caaaagagat attagagctt cctaaaaatg
     1741 aagtaaatat caataaacag attaataaaa gtgataaatt tcttttcatt gtgagcctcc
     1801 aaattttaaa ttcatctttt agtatagcat atatttgtca ataattaaaa gaaaggagtg
     1861 attaatatat gaaaaaaaag gtaaaattta tttcgttttt tgttttattt attttaataa
     1921 tatcatcaat aatcgtttat aaatcgctta ttttcaaaga tgatatttat gttgtaagta
     1981 aaaaaaattt acctaatgaa aaaacacatg ttgtagagaa tgaaattaaa ggtgaagata
     2041 ataatcacaa taaagtaact aaagacatag ataaaaattt tcaaaataat aaaataacta
     2101 tatacataag tggagcagta aatagacctg gaattgtaac tatagagtca gacaagagac
     2161 tttatgatgc tgtagaatta ctaggaggaa cgacaaaaga agcagattta aatggagtca
     2221 atctatcagt aagattagaa gatgaacaac attacataat acctaaaata ggtgaagcaa
     2281 catctgttac aagtaatgat gattccaata aaccaaatca gaagaatgag tcaagaggtg
     2341 agcctaaaaa taaatccaat aaaaatatat ctaacgaatc aaaagtaaat ataaatgtag
     2401 cgactataga agagttagat tctctacctg gggttggaga agctacagcc aataaaattt
     2461 tacaacacag agaagaaaat ggacagttta gttcaattga ggaaataaaa aatgtaaatg
     2521 gtattggaga taaaaagtat gaaaatataa aagatttaat ttgtgtagac taaaatataa
     2581 tacaaaactt acatatagat atgttattat aaatatgtat gtatatgtgt gtacatgtgg
     2641 aaatttagta aaggaggcac ttttatgaaa aaagaacaaa aaaaacttac agagatgaca
     2701 acagcaggtg gttgagccgc taaaataggg ccagaggttc tggcatcagt tttatctcaa
     2761 ttgcctaaaa atgataatat agaaaattta ttagtaggac tagatacagc agatgatgct
     2821 gcagtttata aattaaatga tgatatggca cttatccaaa ctttagactt ctttacacca
     2881 atggtagata acccatacgt atttggtcaa attgctgcat caaattcact atcagatgta
     2941 tatgctatgg gaggcaaacc tttggttgct atgaatattg tttgttttcc atcatgccat
     3001 gatatggatg tacttgcgga aatattaaaa ggtgggtttg ataaagtgaa agaaagtgga
     3061 gcacttttgg ttggagggca tactgtagat gacaaagagc ctaaatatgg tctttcagtt
     3121 tctgggattg taagtccaaa taaagtttta tcaaatgcta ctgcaaaacc tggtgataag
     3181 ttaattataa caaaaccaat tggagtgggt gtattaaata cagccatgaa agaaggtatg
     3241 gtagaacaac atatagcaga taaagtaata gaaataatga tacatttaaa taaatatgca
     3301 gcaatgagtt ttgagaaatt tgatgtaaat tcggttacag atataactgg ttttggtcta
     3361 cttggtcata ctttagaaat ggccaaagct tctgaagtaa gtattgaaat tgaatcaaaa
     3421 catgtaccaa ttattgaagg tgcaatagaa atggcacaaa tgggaattat acctgctggt
     3481 atgtataaaa atatgcatta tgtatctaaa gatgtagaag ttgtaggaaa tatagaagtt
     3541 gcagttcaag atattttata cgacccacaa acatcaggag ggttgttaat ttcagtaaaa
     3601 gaagaacttg cagaggaact tgtaaaagat atgaaattaa atggagcaat agaagctaaa
     3661 attataggtt ctgtagttcc aaaaggggaa aaatatatta cagttttata aaatattata
     3721 gagaaagagt gattgttttg agcaaaagag aattgtttgc aatgttgcct tcagttgatg
     3781 aagtattaag tgataataga attgtagaaa taataaatga atatcctaga agtttagttt
     3841 tagaatctgt gagagaagta atagatttaa aaagacaatt tatattaaga ttaaaagaag
     3901 atgcatcaaa ctctgtaaca atagagtttg gtgaaataat agaatctgca atagagagag
     3961 ttaagttaaa ttactcatta tctttaaaaa aagttataaa tgcaactggt acagttattc
     4021 atacaaattt agggaggtct ttacttagtg aagatataaa agatgaatta tggtgtgctg
     4081 cttcaaggta ttcaaactta gaatacgatt tggataatgg agaaagaggt tcaagatact
     4141 ctcatttaac tagtacaata aaaagactta ctggagcaga agatgtacta gttgtaaata
     4201 ataatgcagc ggcagtactt cttgtactta gcacaatggc taaaggagga gaagcaattg
     4261 tatctcgtgg agaattagtt gaagtaggtg gttcatttag gattccaagt ataatggctc
     4321 ttagtggtgc agaattagtt gaagttggtt caactaataa aactcatttg aaagattaca
     4381 aggaggccat tacagaagat acaaatgtat tgatgaaggt tcatacaagt aattatagaa
     4441 taatgggatt cacagagagt gtttctatag aagaattggt taatttaggt aaaaaatata
     4501 aactacctgt aatagaagac ttaggaagtg gagtttttat agatttatca aaatacggtt
     4561 tatcttatga accaactgta ttggattcta ttcgacaagg tgcagatgta gtaactttta
     4621 gtggagataa gatgcttgga ggtccacaag caggtattat agttgggaaa aaagagtata
     4681 tagaaaaaat gaagaaaaat caacttacta gagcacttag agttgataag cttacaatat
     4741 gtgctcttga agcaacattg agaatgtatt tagatgagac aaaagctata gaaaatatac
     4801 ctactttaaa aatgttgaca tataaaattg aagaattaga agtaaaagca aataaactat
     4861 ttgaaaaaat aactgcttta aacttaaatg caaacataaa tatagaagat ggattttcac
     4921 aagtgggagg agggtctatg cctcttgaaa ctataagtac aaaggtaata tcaataactc
     4981 ctgaacatat gaatgtttca tctttagaaa aaaaactaag acttagtgaa gcacatataa
     5041 tagccagagt gtatgataat aaatatgtac tagatgtaag aactatattt gatgatgaat
     5101 ttgatgtaat agttgcggaa ttgagaaaag catttaatta aataaatttg agctaataaa
     5161 tattaaattt agaagtataa gtatgaagtt ttgattaaaa aatagctaca cacacttgtg
     5221 actttagtca tagcaggttt agagggggca aaaaatgaaa aatgtagtaa ttggaacagc
     5281 aggtcatatt gaccacggaa aaactactct tataaaagca ttgactggaa gagagactga
     5341 tacattagat gaagaaaaaa agagaggtat ttcaataaac ttaggattta cttattttga
     5401 tttaccaaac aatagaagag ctggaattgt tgatgtccca ggacacgaaa aattcataaa
     5461 aaacatgtta gctggagcat caggaattga tatagtttta cttgttgtag cagcagatga
     5521 aggggttatg ccacagacag ttgagcattt agatattcta agctttttaa atatcaaaaa
     5581 tggaatagtg gttctaacta agtctgatac tgtagatgat gaatttagag agctagtaaa
     5641 agaagatatt tgtgaaaaaa taaaaggaac ttttcttgaa gatgctgaaa taattgaagt
     5701 tgattctata tctaaaagag gtatagacac tcttattgaa aaaatagata aaatgagtga
     5761 tgatatagaa gataaaaatg aaaactctcc agcaagactt aatatagaca gagtattttc
     5821 aataaaaggg tttggtactg taataacagg cactttaata gaaggaaaga taagtataga
     5881 agatgattta gttatatatc cacaggtatt aaaaacaaaa ataagaagta tacaagttca
     5941 tggagaaaat aaagatacag cttatgcagg tcaaagaaca gcaatcaata tatcaaatgt
     6001 aaaagtggat gatataaaaa gaggagatgt tttagctgca ccaaactcat tagaggaatc
     6061 aatgatgtta gatgtaaaat tatcattggt aaatcattta gataaaggat taaagcactg
     6121 ggatagatta agattgtatc atggaactag agagatactt tgtagagcag ttccactaga
     6181 taaagaatta attgaaagtg gagaaagtgg ctatgttcag ttgagacttg aagaaagcat
     6241 agtatctaag aaaggtgata cgtttgtagt gagacgttat tctccaatgg aaactatagg
     6301 tggaggtgta ataattgacc catctcctaa aaaacataag aaatttgatg aaaaagttat
     6361 agaagcatta aaaataaaag aaaaaggcga actaaaagat ataatagaag agtacttaaa
     6421 aaggaattta aaaaattatc caaatataaa agaaataatg agctatagtg gtgctcatga
     6481 agaagatgta aaaagagcgc ttgaagcact tatatcagaa gataaagtat ttattatagg
     6541 aaatatgtat atgcatataa atcaatacaa taaattaaaa gaaaatacaa taaagctttt
     6601 aagtgagtac cataagaagt ataggcttag aaaagggata ctaaaagaag aagtaaggtc
     6661 taagatagag agtaatttta agactaggga gatggatatt ttattagaaa aattgtctac
     6721 agaaaatgct ataaaaatag aaaataatat agtatctcta ttagattttg aggttatttt
     6781 aaatgataag caaaaagaaa tagcaaaaaa aatagaaaaa agattaaaat catgtggagt
     6841 ttcttctata ctgacaatag atgaagtttc tgaaggaaat cataactatg ctgaagtatt
     6901 ggagtcaatg ataggtaata aagtagagaa attagatgat ttgtatataa tggacaaaga
     6961 tatttatgaa aatgcaaaga acattttgat aaattatata aaagaaaata aagaaataac
     7021 attaggagaa tacagagatt tgattgattc tagcagaaag aattgcatga ttatattgga
     7081 aaacttcgat agaaacaaaa taactaaaag agtagaaaac aaaagaattt tgttttagca
     7141 gaaaatgtct gtttttgctt taaggtataa agaaggaatt ataatgtcga gtatcgtata
     7201 attaattagt ataaaatatt aattaagagg aagtgtaaag tattgaagaa tataatacct
     7261 gatgtaaaac cagatataaa agaaagagaa gacatgagcc ttttatttat aataatgggc
     7321 tcagtacttt tattatttat tataaaagat atagagaaat atgaaacttt attacatgcc
     7381 atatatacga tatttttagt agctacagga gtaattatat ttaatactac aaaatttaaa
     7441 gtagattcat tttctacatt tttaggatta atttttatag ctactggagt attggagtgt
     7501 atatatttat ttaattcctt ggggattaaa actaagtcta taatggagat taacatcaca
     7561 atatcagcaa taacagattt atttcctata ctgggtgtat acttaagttt taaatttgtt
     7621 aaggataaca aacaaattta ttcaagtata attttattta tagcaactgc tacattaaca
     7681 ataacatctt tattcataat atcaggaatt tgtgactatc tgggaaatgg taaatcacaa
     7741 tatgctttag gagttataat atctatattc ataataatta tttctatcat tagtggaatt
     7801 gaattaaatt caagttcaaa taaaggtaaa tgggaatatg atgaaaaaaa gatttttaac
     7861 agaataatag taataataat tttatcaaga gttccaaatc tattgcatat agttattgat
     7921 aatagaaatg tagagcggat attatcacag ataataataa atgtagcctt atattattta
     7981 tataattaca tagtatctaa gaatattaag aaaacagcac tggaattgca tgatacgaat
     8041 gaagaattaa caaaaaaaac ggaaagttta aaagaaaaga ataaaaaatt aatctatgag
     8101 acagaaaaga tagaggaact taagggaata cttatgaaaa gagaatctag gctacaatct
     8161 acattagatg tagcagtaaa tagcatagta gtgtttagca aagatggaga aattacatat
     8221 gcaaataaga gctttagaaa tacttttgga gactataaag aacaagataa attgactgat
     8281 aaaataaaaa acttcaatga tttgatagaa agcattcatt atgtgtttgt tcatgagaag
     8341 aatgcagaga aacttattag tacaagcgat aataaagttt atcaagctat atttgcacct
     8401 ttaattattg cttcacaaac agaaggagta ttgtgtgtac tagaagataa gactaagaaa
     8461 aaggagtacg aacgcaaact tattgaagca aataagagaa gtaaagattt tttagaaagt
     8521 attggagatg gcatagtagt gcttgaaggt aataacaaaa tatatgttaa taatgcatgt
     8581 agggaaattt ttaaaaataa tttagagagt attgattttt cattagtatg taaaagtgaa
     8641 gaaagtatag aaaaaagata tgtaatagat ggtgaagtaa agtatgtaga gatgagtttt
     8701 tctcagtata ctaatgctgg tactaataaa actattattg taatacgtga tacaacctct
     8761 cgaaaaatat ctcaaataaa acttgaggaa agtcaatcat catattcaag atttatagat
     8821 atattacctg atggcatatg cttattaaaa gaagatttaa gtataaacta tgcaaataag
     8881 tcgtttttag atatgctagc ttttacaaac atagatgata taaaagattc taatattaaa
     8941 ctattaatga atgcgaatac tgaagaaaaa atgaaattta cggataatat ggctaaagtt
     9001 ttaaatgaaa atagaagtat acttttactg gaatatgaat tgataaatag ttatgatgat
     9061 atagtagaag tagaattaag tgcattacct tttgctatat acaatactag atacataatg
     9121 cttattataa aagatttagt acataagaaa tattccgagc aagcagaaaa agaactgctt
     9181 gaaagattta aaacagataa aatcaaaacg gagttttttg caaatatgtc tcatgaatta
     9241 aaaactccac taaatgttat atctagttca aatcaactgg tagattcttt ctatagaaat
     9301 gaaaagataa aagattacaa taacaatatt aaatctcatg tggatttagt tagacaaagt
     9361 tcttataggc ttcaaagatt aataaataac attatagatt taacaaagat ggagtctggg
     9421 ttctatacac taaaacttgc gaaatacaat attgtatctg taatagaaga cttatttatg
     9481 aatatagaag agtatgcact tagaaaagac ataaaaattc tttttgatac agaccttgaa
     9541 gaaattaatg tttatataga caaggttgaa attgagagaa ttatgttgaa tctattgtca
     9601 aactgtatta agtttactga taatggaggc tggatatatg taagtattca ttataaaata
     9661 gataaagtaa tcataagtgt aaaagacact ggagttggaa taccacaaga taaattagaa
     9721 ttgatttttg aagaattttc acaagttgat aagactttat ccagaaatac tgaaggaagt
     9781 ggaatagggc ttgctatagt taagaatcta gtaagtcttc atggtggaga tatagaagta
     9841 gtaagtgaag tgaataaagg cacagagttc ttaatttcac ttccaatgaa gggatttagt
     9901 agtgaacatt acacagaaga taagagaata tataatattc aagaaaaaat aaaaatagaa
     9961 ttttctgata tatactatta aaaatgatta tgagcaaaaa taaaataaaa atactatttg
    10021 tttaaatatt taagttgtaa gaaataggct atcataaggt aaattattat gatagcctat
    10081 ttttagctag gttttatatc tatatgtgag aacatataat ctctaaatta ccttcaaaac
    10141 taaaatctcc aaagtcgttt ggcagtataa aatgcatacc cttttttaaa ttatattcta
    10201 cagaattgtg tataagtttt ccatttccat ccaacacact gtataaagaa aaaggtgtat
    10261 tgtgtgtaaa attacatttt ccaaaaacat ctaatttata aacactaaaa aattcgtttg
    10321 acacaaatgt tgtgcattta aagtctgatg ttgatacgat tttatagtca gtatcgaaat
    10381 ttatatgagg aacattcgta acatctattg atttttgaac atgaagttca cgcttattac
    10441 ctgaattgtc agttctatca taatcataga ctctataagt agtatctgag ttttgttgag
    10501 tttctaaaat caatgttcct ttgcatatag catgtatagt accacttgga acatagaaaa
    10561 agtctccctt ttttatatta acctttctaa gtagtttgtc ccattcatta ttatttacca
    10621 tatttacaaa ttctttatga gattttgcat tatgacctat tataatttct gcatcatcag
    10681 aacaatctat tacataccaa cactctgtct tacctaactc tccattttca tttttcttag
    10741 catattcatc atttggatgg acttgaactg ataaattatc atttgcatcc aatatttttg
    10801 taagtaaagg aaacttatca ccaggtgtgt ttccaaataa ttctctattt ttattccaca
    10861 attcagatag ttttttacct ttatattttc cattttctat taagcaatct ccatttttat
    10921 gagagcttat agcccaacat tcaccagttg ttggcgaatc tatttcataa ttaaatttat
    10981 ctttaagtgc agtaccaccc catattctat ccataaaaat aggttttaaa aataatggtt
    11041 ccataatcct gttcctccta tgatattaaa tttatcctac aaataattat atataaatag
    11101 attataaaat caatagtaaa aaagttttgt cccttgaaat gacataaagt ataaggagtt
    11161 attaaataag ataaactatg atatataata taaagttcta aattggagtg ataattatgg
    11221 agttgtttaa ggcagaaagc aaaaaatatg aaaaagaatt tttaaaatta ttggagcaat
    11281 gggttagtat accttcattt tatgatagaa aaactgtatc taaagatatg ccttttggaa
    11341 aaggagtata tgatgcatta aactggtttg aaaatctagg tagagaaaac aatttcaaag
    11401 taaaaaatat agataatcat gctgttcaaa ttgaatatgg aaatggtaaa gaatatgtag
    11461 atatatttgg acattgtgat gttgtaaatc caggtgaagg atgggatagt gaaccattta
    11521 aactaaatat tataggtgat aaattagttg ctagaggtgt atctgataat aaaggaccta
    11581 tgatagtgaa ttttttagca ctaaaaatga taaaggattt agatattaat ttaaaaagaa
    11641 aggttagatt aatagctgga ggaaatgaag agagtggatt taaatgtata aagcattatt
    11701 atagcaaaga gccatatggt gtatgtggat ttactccaga tgctaaattt cctgtattaa
    11761 atggagaaaa aggtggagca ataataaagt taatttcaaa tatagacgat aaatctttat
    11821 atataagtgg tggaatagag tttaatacaa taccagataa agtttatata aagaatgtag
    11881 aaaaattggg taaagataat atatgttttg atattaacaa tatatcgata aattatgata
    11941 atggcaatta tattgtgtat ggaaaaggtg gtcattcatc taaacccgaa aaatcaataa
    12001 atcctatatt agctacaatt aaacttttgt cagaaaatat tgatgaaaaa tggacaaaag
    12061 atttatataa gttgataaat caagataata taaatggaaa cttatttggt ttaaatattg
    12121 aggggaaatg tgggatttta tcaatagtgc caacaataat aaatatagta gatggaaaat
    12181 tagaagtagt tttaagcgtt agatatccag aaatattaac cattgaagat ataataaaaa
    12241 aatttaattt gtatatggag caaaataata ttaataaatt tgagcttatt ggggaaaatt
    12301 taaagcaagc aaattatata gatagaaatt caaaacttgt aagaagtttg catgatatat
    12361 atataaagta ttctggagat ttgaaaaatg atgttagagt aactagtgca ggaagttatg
    12421 cttctgaaat gaataattca gtaatatttg gttgtgaatt tcctgatggt agctttggca
    12481 atgtccatag tgcgaatgaa tttgctagct tagatagatt tataactgca ataggtatat
    12541 atgcagaggc aataattaca ttatgtaata aaatataaat acaaaatgtc ccttatatag
    12601 acatattata tatacaatta aaattttact tgatatataa ttgaaatcaa agagaaggtg
    12661 atacaatgat aactaagaaa catgcaataa tagtaaagag tttaagcaat aaagatggat
    12721 atatgacatc aaacgaatta gctatcaaat tggatgtgtc tactaaaact attaaaagat
    12781 atatagcaga tttaaatagt gttttaagca aatatgattt ggtaatagct tcttctagag
    12841 gtataggata caagttaagc ggttctaaaa acaatatagc cagagctgtt aaagaagcaa
    12901 ataaatatat tgatggtttt ttagatgact ctgaagagtc aagaatgtca aatataatat
    12961 gtatgcttat taatagaaat tatatgagta tagaagcaat ggcagaagaa ttaaatctaa
    13021 gtatagccgc tataaataaa ttgtctagta agcttaaaag aaaacttgaa aagtatgact
    13081 tagttataaa atctaaacct tactatggaa gctatataca tggtgaagaa attaatataa
    13141 ggcaattaat tactgactat gcaataaaat tagatgaaaa aagtaaagtg aaggtgtttt
    13201 tagatgacat tagtgaaaac gatatatact gtatagaaag tattttagaa aagcacttaa
    13261 gagaaaaaga tacaataatt tcagataaag attttaatct tttgctttca aaaataattg
    13321 tttctgtatt taggagtaag agaggtcata gtaacaatat aaacttaatg gatacaagtt
    13381 atagatttca taactactac tttatagaaa atctaatgaa agaaatatct gataaaattg
    13441 gatttaaact tattgaagat gaagtaattt atatttcaaa ttattctggt gtaatagctt
    13501 ataaaggtac acaaggaaga aaaaacacaa gtgaaattga ggaacgaata agtagtgtta
    13561 taagtagtgc acttcaagat atattcctta tttcaggtag tgattataca aaagatgatg
    13621 agtttatggt tgcaatctct gaccatataa agagattttt aaatagagct agagctaatg
    13681 taaaatcaaa taatccttta cttcatcaaa taaaagagaa gtttcctata gcttttaatt
    13741 tagccgtgtt tttatcaaat aaactagaaa ctgagtttaa tttaaaactt gatgaagatg
    13801 agcttggata tatagctatc cactttgctg cttcaaatga aagaatgaaa aaaaatacta
    13861 gtaagaaaat ttgtatagtt tgtcattatg ggataggaac aggacaactt atttctgaaa
    13921 agctaaaaca gaatataagt gatttaagtg ttgtaggtgt ttatccagtt agatatcttg
    13981 atatggctat aagtcaagat gttgatttga tagtatctac cgttgaatta aaaggatatg
    14041 aaaaacctgt cttatatata gaaaatatat ttgatgatag cttaattgaa aacgtaaaca
    14101 aagcctttta tgaaaaggaa gaaagaagaa agataataag taacatgttt gatgaaaaag
    14161 cttttttcag tataaaggct agtactaaag aagaggtcat aatttcactt tcaaataaac
    14221 taaaagaaag agattttata gaggaaagtt ctataaaaag tataatagat agagaaaata
    14281 tttcatcaac tgaaatagga aatctagtag caatacctca cacaatagtg aagggagata
    14341 aaaaatctat aataggagtt ggaatacttg aaaatccaat aatttgggat aagcaagagg
    14401 ttcagctagt atttatggta ttttttaata ctaaagaaaa gcataatttc tcgatattta
    14461 aataccttta taattttata aaagatgaag gaggagtaag aggaattatt aaaatatgtg
    14521 actttaataa attaatggca ctgataggta attaaggagg aaattaataa tgggaacaaa
    14581 catatttaac aaagaatatg tatttttaaa tgttgatgct aagtcaaagg ttgaagtttt
    14641 aaaatttata tcaaaaaaag ctaaagactt aaatttggca gaagatgaga gcttagtata
    14701 tgaaggttta atggctagag aagaacaatt tactacaaat ttaggagaat ctatagcaat
    14761 acctcacact aaaaatgatg caattgaaaa tccagcagta gtagtattga agtttaatga
    14821 agatgttgtt tggaatgaag gtgaggataa ggttaaatta gcaataagct tacttatgcc
    14881 aggaaaaagt aaagagaata ttcatcttaa attattatca tctctttcaa gaaaattaat
    14941 aaataaagaa tttaaagatt ctcttttaaa aagtgataat gttgaagaaa tatctaactt
    15001 aataaatgaa gctttaggtt tataattaaa tactaaggtt ataaaataaa aaaataatta
    15061 actaaaaata ggaggaattt gaaatgaata aaaaattagt agcattatgt gcttgcccaa
    15121 tgggactagc tcacactttc atggcagcag aagcaataga gcaagcagca aaggcattag
    15181 gttatgaagc aaaagttgaa actcaaggtg ctgatggtgt acaaaatgag ttaacaagag
    15241 atgatatatt aggagcaact atgataatac atgcagtagc aataacacca gaaggtatgg
    15301 aaagatttga tggttgtgaa gtttatgaag ttgaattaca agaagctata aaaaatgcag
    15361 aaggtgttat aaaagaaata gaagaagatt taggaatata ataaatttat tgtggaggaa
    15421 atagaatggc aattaagaaa aaagttataa acagttcagg tgcttctaca acaggaggaa
    15481 ataatgtagg aaaatctact ccatctagtc caactcaatc aaagggaaat ggaaaatgga
    15541 aagaaataag taaacatata atgacaggta tatcatatat gataccagtt ttagtaatgg
    15601 gtggacttat aggggcctta tctcaattaa taccatatgc tatattagga cttgacccat
    15661 ctgtaggtat agttgatgct atgaactcag gagaatttac agggttcaag cttagtcttt
    15721 taaatatagc acaattaatg tcaaatttcg gatttacttt gtttggattt gcgataccac
    15781 tgtttgcagc attttgtgca aactctatag gtggaaaaac agctttaatt gcaggattta
    15841 taggtggata tatagctaat aaacctgtag gtgtaccaca attcattgat ggacaatgga
    15901 ctgaagttgt tccagttgca tcaggatttt taggtgcaat actaatagca ttcataatag
    15961 gatattttgt aaaatggtta aataaatcta taaaagtttc tcataactgg ttagctttta
    16021 aaacaacatt tttaatacca ttaatagcgt cattagcttg tatggtattg atgatattta
    16081 taataactcc atttggtgga ttaattaatg aaagtatgaa aaacttctta acagcagcag
    16141 gagcagctgg agaatatgta tatgcaacag ctttggcagc agctacagca ttcgacttag
    16201 gaggaccaat aaataaagca gcaggattcg ttgcacttgg tttaactaca gagaatgtac
    16261 ttccaataac agcaagaaca atagctatag ttgttcctcc aattggatta ggattaacaa
    16321 cattattaga taaaagatta gtaggaagaa gagtatatga tagacaattc tatcaagcag
    16381 gaaaaacttc tatattctta tcatttatgg gtatatctga aggagcaata ccatttgcac
    16441 ttgagagacc aggttttgtt atacctttaa acatagttgg aagtgtaata ggagctatta
    16501 caggtataat tctaggagca attcaatggt tcccagagtc agctatatgg gcatggcctc
    16561 tagtagataa cttatttgga tatataatag gtatagcagt tggtgctata ttcatagcag
    16621 taggaaatat attctataga aataaattaa taaaagatgg taaacttgtt gtagattata
    16681 tcgactagta tttaattttt actatctata aataactttg gctggtaata tggttttaat
    16741 caatattacc agccttacct atatgattaa ttaaaggcgg tggagataag atatggataa
    16801 gatagaaaaa gtaagagagt attttagaga atatgatata gatggatttc ttataaatag
    16861 tcctacaaac aagttttatg ttggaaattt atttagctct tcaggttatg tttttataac
    16921 gaaagaatca caatatataa tagtagattt tagatatttt gaagaaatta aaagaaaatc
    16981 tagtttgttt aatgtagtat taatggacaa aactagaact cattttgata taattaatga
    17041 tatttgtaga gagcagaata taaaggaaat tggatttgag ggaaatgaag tatcttttga
    17101 tttatataga agtatgagta ataagttaag tgcaacctta aaatcagttg atttatctac
    17161 attaagggaa acaaaaaatg aagacgaaat aaaatatata aaaaaagctt gtgaaatagt
    17221 tgatgctaca ttttatcata tagtagactt tataaagatt ggtatgactg aaaagcaagt
    17281 tgaaaatgaa attgttagaa ttataaaaga tttaggtggt caaaaagaat ctttcgatac
    17341 aatagttgca tctgggctta gaggagcatt acctcatgga aaagcaagtg aaaaaattat
    17401 agagtatgga gattttgtta cttttgactt tggtgctaag tataataatt attgttcaga
    17461 tattaccaga accatttgta tgggtactat aaataaagag ttagaagaaa tatataatat
    17521 agtaagaaaa gctaatgaag agtgtataag agttttaaga ccgggaatga ctactggaga
    17581 gatagataaa gttgcaagag acataattgg ttcatatgga tatgccaata attttggtca
    17641 taatctgggt catggtgtag gaattatggt tcatgaatat cctgccttag ctcctgaatc
    17701 aaatgaagta ttaaaagaag gaatgattgt aactatagag cctggtatat atgttccaag
    17761 tcttggaggc gtaagaatag aagatgatgt actcataaca caagatggat gtatgagatt
    17821 gactacatct actaaggatt taatagttgt aagctaaaaa taaattagat aaatatttta
    17881 taacaataag aaaagaagag gtaaatttaa atgtataatt ttgatgataa accagataga
    17941 gtaagtgaaa agtgtagaaa atgggattta aatattataa gagataaatt tggagatatt
    18001 agagaagatt ttgtaccaat gtggattgct gatatggatt ttaaaatacc aacagaaata
    18061 gaaaacaaat ttattgaagc tgtaagaaga ggtgtatttg gatatacata ctgttatgat
    18121 gaattttatg attcagttat tggttggcaa aaaaatatgc atgaggtaaa tgtagaaaaa
    18181 gaatggatta cattaactta tggaactgta tcaacacttc attatgtagt acaagctttt
    18241 tgtaagaaag gtgatagtat aatattaaac acaccagttt atgacccctt tgaatcatca
    18301 gcaaaaaaac aaggtgtaaa tgtaatttgt aatacattag atgttgtaaa tgatagatat
    18361 tatataaatt ttgataaatt agaagttcaa attaaagaaa ataagcctaa attaatgatg
    18421 ttttgtacac cacataatcc atctggaagg atatggacaa ttgaagaaat gacaagggta
    18481 gcaactatat gtaaagaaaa taatgttatt ttggttgcag atgaagtaca tgcagagcat
    18541 attcattatg gtaaatttaa ttcaatactt aagattggaa aagagctttt agaaaatgtt
    18601 attctactga catcaccaaa taagggcttt aatcttggtg gattaaagac ttcttattca
    18661 atagttataa ataagaatat aagggataaa tttagaaata aattaaagca aaattctata
    18721 acttctccaa atgtgtttgg aataatagga ttgataaccg cttataatga atgtcatgag
    18781 tggttaagtg gtgtaaatga atatataaaa tcaaattatg aacttttaga aacttgggta
    18841 aataaatata ataagataaa attgatgaaa atggaatcat cttacttagc ttggatggat
    18901 ataagtggat taggtataag tgcaagtgag tttacagata aattggctat agatacagga
    18961 gtattattag aagatgggag tcattttgta aaagatggtg agaagtatgt tagaataaat
    19021 ttaggaactc aaaaggaaaa tgtaattgaa gctttaaata gaatggattt atttttaaag
    19081 tctttataaa aatttggttg aattagtatt ttatataaat ttaagctatt aatatggtat
    19141 atacataagt agaatttgtt ttagattact taagtatata ccatatttta tataaaaatt
    19201 aaatttgcat tagtcatgtg ataactattt a
//
LOCUS       DAMJVX010000062        19195 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMJVX010000062 DAMJVX010000000
VERSION     DAMJVX010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.065"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(23..865)
                     /locus_tag="PC407_001948"
     CDS             complement(23..865)
                     /locus_tag="PC407_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2497394.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEISTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(1112..1606)
                     /locus_tag="PC407_001949"
     CDS             complement(1112..1606)
                     /locus_tag="PC407_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2497395.1"
                     /translation="MSTLVTLLGLLVCTKISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKLSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPRMPLSVSLALAAVISSTDLVAVKSITQGLNFPKNMMSILEGESLLNDDDRII
                     NIME"
     gene            complement(1758..2729)
                     /locus_tag="PC407_001950"
     CDS             complement(1758..2729)
                     /locus_tag="PC407_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDF2497396.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPDEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTRALMSAVPVQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(2730..3707)
                     /locus_tag="PC407_001951"
     CDS             complement(2730..3707)
                     /locus_tag="PC407_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2497397.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMVIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPVLGKNKDKRLYSIPGQV
                     PNPIGIPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            3926..5485
                     /locus_tag="PC407_001952"
     CDS             3926..5485
                     /locus_tag="PC407_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2497398.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKRKEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            5591..6550
                     /locus_tag="PC407_001953"
     CDS             5591..6550
                     /locus_tag="PC407_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2497399.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            6553..7365
                     /locus_tag="PC407_001954"
     CDS             6553..7365
                     /locus_tag="PC407_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2497400.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVISLTIISKKFSSNT"
     gene            complement(7580..8482)
                     /locus_tag="PC407_001955"
     CDS             complement(7580..8482)
                     /locus_tag="PC407_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2497401.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            8837..10015
                     /locus_tag="PC407_001956"
     CDS             8837..10015
                     /locus_tag="PC407_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2497402.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINSGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            10024..10680
                     /locus_tag="PC407_001957"
     CDS             10024..10680
                     /locus_tag="PC407_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HDF2497403.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            10680..11345
                     /locus_tag="PC407_001958"
     CDS             10680..11345
                     /locus_tag="PC407_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HDF2497404.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     ILGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            11450..12226
                     /locus_tag="PC407_001959"
     CDS             11450..12226
                     /locus_tag="PC407_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2497405.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            12288..13622
                     /locus_tag="PC407_001960"
     CDS             12288..13622
                     /locus_tag="PC407_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2497406.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVLLNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKVGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSKLEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            14119..15045
                     /locus_tag="PC407_001961"
     CDS             14119..15045
                     /locus_tag="PC407_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HDF2497407.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPNNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSENFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(15437..18976)
                     /gene="nifJ"
                     /locus_tag="PC407_001962"
     CDS             complement(15437..18976)
                     /gene="nifJ"
                     /locus_tag="PC407_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDF2497408.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHACIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRIQV
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKVDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVLEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         6671 a         2999 c         2732 g         6793 t
ORIGIN      
        1 ttctgtatta aactactctt tattataaat tcagtacaga tataactctt ttgatatgta
       61 atgttaaata aagtagttca tcttgactta catagtaatc ataattcttt tctacatgag
      121 tttttatttt atatgcacaa tcataatcct tttcatactt tactttaatt atatctataa
      181 tttcattatt tgtatcattg attttttctt tttttactaa tcgttttgca aagaatttta
      241 aatgagtcaa aagcctatca taatttatat catcttcttt aaattcaact ctataataat
      301 atcttataat atttagtatg tcttttacaa ttttagtaat taacagtgat tcctttgaac
      361 ttcccttata gtttgaattt actatatgca ttgctataaa accagcttca tctactggaa
      421 atttcacatc aaattcttta tttatataat ctacagccca tttaccaatc tcatattctt
      481 ttttatgtat tctttttatc tcatgtagta aatcattctt tatagttata ttttcatgat
      541 atcttttaat tgcaaatgca atatggtcag taagtgctac ataaatatat tcataaagct
      601 tagtattcaa attctcctct gcataagcaa ttatttcagt actaatctca aaaaatttct
      661 catcaacctg gcttgctagt ttgctaattt tatctaaaaa gctttcatct tgtattacaa
      721 aagttctttc aattttactt ttatctattt cttgaccttt ctttttttga aacccaatcc
      781 cacatcctgt tagtattaat tcttttttta tattttcatc taaaactaat acaacattat
      841 tattataaat ttgttgaata atcataatca atatctctcc caaaatacaa tttcccttat
      901 tcataaaaaa atacctaaat taagatacaa ttcacctgta taccttaacc taggtattgc
      961 ctgcttacca gtaacaatcc agcttgttaa ttcttatata ttgtattcaa tttatggttt
     1021 tattctatta taaataaata tatatgtcaa gtaaattatt gaaaactatt tcctattttt
     1081 tagtattatt tttaacatca aaaatcaaat tttattccat tatatttatg attctatcat
     1141 catcatttaa taaagattca ccttcaagta tactcatcat atttttagga aaatttaatc
     1201 cttgtgtgat tgattttact gctactaaat cagttgaaga tattactgct gccaaagcaa
     1261 gtgaaacaga taatggcatc ctaggaataa gaaaatgtat aaagattcca cctgcaaaca
     1321 cggttatcaa aactaaacca aatgcaagaa gcaaaattgg ttttctaagc tctagtaact
     1381 cttttcttga tacattttgt ccttcactga ataataatgg agctatgata catattacaa
     1441 atatttctgg attaaatgaa aatgataatt taaacggcaa aatagacaat ataatcccta
     1501 gtacaatttg atatattgca agaggaatat tactccactt tttgctaaat acagttgaaa
     1561 tctttgtaca aaccaataat cccaacaatg ttactaaagt tgacataact acctctcttt
     1621 tcaataataa tattataaat taaacttata tatcaactaa cttaatatta acaacatgaa
     1681 atcccatctt aatcaaaaaa attttcagtt tattctatta ttcttattat atacatcaaa
     1741 tttaattttt gaaatatcta tactagatgg catgcaacaa aatgatttct tgatatttct
     1801 tttaaaactg gaatctcttt tttgcatatt tccttacaat aagggcatct tgtatgaaac
     1861 ttacatccac ttggtggatt tgttggagat ggtatgtctc cttttaatat aattctattt
     1921 ttcttaattg tagggtcttg tactggtact gcgctcataa gtgctcttgt atatggatgt
     1981 tgagaatgta agtaaagttc tcctttagtt gcaatttcta ttatagaacc tagatacata
     2041 acacaaactc tatgtgcaat atgatttact acactcaaat catgagatat aaataaatat
     2101 gagagtttat atttatcctg taaatccata agtaaattta taatttgaga ttgaatagaa
     2161 acatctagtg cagaaacagg ttcatcacaa actataaatt ctggatttaa tgctaatgct
     2221 cttgctatac ctatcctctg tctttgacct ccagaaaact catctggttt tctatttaaa
     2281 tggtacttag aaagaccaca actttctaca acatttaata tattatcttt tatttcattc
     2341 tttggcacta aattatgttc tataactgcc tctgatatta tttgttccac atttaatctt
     2401 ggatttaatg aactatatgg gtcttgaaat atcatttgca ttttgggtct taactttcta
     2461 agttcttcct tctctaactt atatatatct acaccattaa attcaacttt accttcagtt
     2521 ttttcaatta atctaaggat ggttcttcct gcagttgatt ttccacatcc cgattctcca
     2581 actatagcca aagtttctcc tttttttaaa tcaaaagaaa ttccatctac tgcctttaca
     2641 catttaattt ccttatcaaa taatccagat ttaacttcaa agtacttttt aagaccttct
     2701 acttttaata aactctcttc ttttttcaat tatatctcct cctttttata taaccaacat
     2761 gcaatactat gaccactatt caattctatt aaaggaggta tctgagttct acatttatca
     2821 catacttttt cacatctatc actaaaataa catgaatctg gtattccaat tggatttggt
     2881 acttgtcctg gaatcgaata caatctttta tctttgtttt ttccaagaac aggctttgat
     2941 tttagaagtc caatagtata aggatgtttt ggatttttaa ataattcaac aacctctgcc
     3001 acttctataa ttttaccagc atacatcact aatactttgt cacacatttc tgcaactact
     3061 cccaaatcat gtgtgatcat cattatagat gtgtttaatt tttcttttat atttttcatt
     3121 atatctaaaa tctgagcttg aatagtaaca tctaaagctg ttgttggttc atcagctata
     3181 agtaattttg gattacatga taaagccata gctatcatta ttcgttgtct cataccacct
     3241 gataattcat gtggatatga attatatatt tcttcagctc ttggtatttc taccagtttt
     3301 atcatatcga ttaccttttt cttagcttct tcctttgata aatcttgatg tatcataata
     3361 ccttcaatga tttgacttcc aacagtgaac gctgggttta aagatgtcat tggttcttga
     3421 aaaatcattg atatctcatt ccctcttatt accataagtt catcttcatt catctttaaa
     3481 atgtctttgc ctcgaaagat tatttctcca ccttctattt ttgatgttgg aagtaacttc
     3541 atcaaagaca tggctgttac agacttacca catcctgatt ctccaactat tccaatagtt
     3601 tcaccttcat ttatattaaa acttacttca tttacactct ttacaatccc tttatctgta
     3661 tgaaaataaa cttttaaatc tttaacctca attagtgctt tactcattat tttctcctct
     3721 cagcaaaatt acttaaaata acatacacct tttgattgta ttttataaaa atttaaatta
     3781 caacattttc ttacatttta ttattttttt ctattaatta taataatata atagttaatt
     3841 tctatatata ttgatttaat aatgattact attatacaat gaaaaatatg tgttaaatat
     3901 tattttatgg aggttttata agaatatgaa atttaaaaaa ttggcatcat taatcttagt
     3961 ttcatcatta atgcttacat ttacagcctg tgcttccaat gataaagaca agtctagtgg
     4021 aagcaaatct ggtggaactg ttgtaatacc tatggcaagt gacccagata taatcaatgg
     4081 agcttttgca aacgttaaag aagactctct tgcatcaaat atagtttatg ctcctcttta
     4141 tacttatgaa aaagggaatt tagtaaatta tttagcagaa aaagtagatt ttaaagatag
     4201 taaggaatta actataaaat taaaatccaa tttaaaatgg catgatggaa aaccaataac
     4261 tgctgaagat gtcttattta catttaatac agttttagac gaaaaacaaa attcaccatc
     4321 aagacaatac ttattggtgg gtgaaaaacc agttaaagta gaaaaaattg atgatttaac
     4381 agttaagatt actttaccaa ctgcaagtga atctttctta tatggtatat ctaaaatttc
     4441 tccaatacca aaacatgtat ttgaaggaga atcaaatata gctaagtcag aaaaaaataa
     4501 taatccagtt ggttctggtg cattcaagtt taaggaatgg aaaaaaggtg aaagcatagt
     4561 atttgaaaag aatgcagatt attttggtgg agaacctaag gcagattcta tagcgctaaa
     4621 aataattcct aatgaagcat ctcaagaagc agctttaaat aatggcgaaa tttcacttat
     4681 gaagacatct gctgaaggat atgaaaaggc aaaatcaaac tcaaacttac aaacttatac
     4741 ttatagtgaa gaaagattaa actacattgt atttaaccaa aatatatcaa acatggctaa
     4801 caaagaagta agacaagcct tatcatatgc cttaaataga aatgaaatga tagaatcagc
     4861 atatggaaaa gaaggttctg tacctgctaa atctatatta gttccagaag cagacttcta
     4921 tacagaagaa ggtgttgaag gatatgacca agatacaaat aaggctaaag acttattgga
     4981 taaaagtggt gttaaaattg ataaattaaa aataggttat aacacaggta gatttgggca
     5041 taaaaactat gccttagtag ctcaacaaga gttgaaaaaa attggtatag aagctgaaat
     5101 agttccttat gaaagtaagg cattctttaa tatactattt agtaatagca ctgaatgtga
     5161 tatgtatgta aatggttatg cttggggatt agaaccaaac ccatatagag gtatgtttga
     5221 aactggtcaa tattgcaacc aaactaaata ttctaatgct gaaatagatg ctttatggga
     5281 aaaaggattt actgagttaa acaaggaaaa acgtaaagaa atatacaaac aaatacaaca
     5341 ggatatatct aaggatgcac ctatttacac tatagattat gaacaaaatt taatggcagc
     5401 tcaaaaaaat ctaaaaggaa taaaagatgc taaaccatct cctgcaatat tatttgaaga
     5461 ttggtctaaa ctgtatgtag aataattgat gtaaaatttt atattattaa ttagaaaagg
     5521 agatgtctaa aaataatatt gttttattat tttaaaacga gacatctctt ttaattagga
     5581 gaatttactt atgttaaaat taatattgaa tagagtaaaa gttttaatac ctatgttaat
     5641 cttaatatct atattatcat ttggactttt agaattagct cctggtgacc cagcagatgc
     5701 atatataaat cctttaatgt cttcacagga tattgaaaat ataagggtta atatggggct
     5761 tgataaacca gtatatataa gatatttaaa ctggttgaaa aacactttaa gtggaaactt
     5821 aggtatttct tatataaacc atatgcccgt tacagaacaa attatggaaa aaatgggaaa
     5881 tacatttatt cttatgggta cttctttgat attttcaata ttggtagcta tacctcttgg
     5941 aatattttta gctgtaaata aaaattcaat aactagtaaa gtatcatctg tttttaatta
     6001 tataggtgtt tctatccctt ctttttggat aggtatgata ctcatttcaa ttttttcagt
     6061 taaacttaac attttcccat caggaggtat gcatactatt ggaaatgata gtattggaga
     6121 tttagtaaaa catttagttt tgcctgtaat aactttaggt ctatataata ctgctatttt
     6181 tacaaattat gtagaagcta gtgtaaatga acaattaaaa aaacaatatg ttattacagc
     6241 aagagcaaaa ggattatcag aaaaagtcat actatttaaa catgtcttga aaaattcact
     6301 gacatcattg gttacaatcc taggcatgag tatacaaaaa ttagtcactg gtgcatttgt
     6361 tacagaagta gttttctctt ggccaggtat gggaagatta atgattgatt caattttttc
     6421 aagagattat actgtaatta tggcaataac tatgttatca gcactatttt taattttagg
     6481 taatttagtt gctgatatac tatacttatt aattgaccct aaaataaaat ctagcaaagg
     6541 aggattctag ttgtgtttac aagtatctta aaaaaattaa aaaaagataa atcctttgct
     6601 tttgcatgta ttactcttag cataataatc atattaatta tagcttcttt tatattaaat
     6661 atagactcta atgcaattga tactacttct aaacttcaag aacctagctt aaagcatatc
     6721 tttggtacag acgaacttgg tagagattac ttctctagag ctctttatgg tggaaggatt
     6781 tcacttagtg ttggtatttt atcaatgctt atctctgtag tatttggtac tgctgtaggt
     6841 gttattagtg gatatattgg aggtaagcta gaccaattct tgatgagatt agtagatatg
     6901 cttatgagta taccaagttt cttagttttg attgtattaa atacttatct aacacctaaa
     6961 gtttccacta ttatactaat tattggattc ttaagttgga tggaagtttc aagaattgta
     7021 cgtggcgaaa cattaaaaat aaaggaaaat gaatactgtt tagctgctaa agctactggt
     7081 atcaagacta gaaatatact attaagacat atagttttaa acttaaaaga gactattgta
     7141 gtcgctggtt cgttaaatat tgctaatgct atcttaactg aatccgctct tagtttttta
     7201 ggtcttggaa tccaattacc tcttgcatca tggggtaata tgcttcaaga tgctcaaaga
     7261 catatttttg acaagttata tttagctgta tttccaggat tacttatatt tttaacagtc
     7321 ataagtttga ctataataag caaaaaattt tctagtaata cttaacaaat tttatataga
     7381 aaataaaaac agtgattatc taaagatata cactgttttt atttttcgtt taaaaaatac
     7441 atattttgta tattataact ttaaatacta ctctaccatg ttatgtaaag cctattattg
     7501 ctctcaaaca tatagatgaa aaactaatag tttattgtaa taaaaattga tttatttatt
     7561 tgttatttta ataacatttt taagagttct ttttactaaa gaaatctata ataaatttaa
     7621 atacatgttg gtctacatgg ctataacgtt cttttttagg tctacatagt ataactttcc
     7681 atgtaatagg atgattaaaa ggcttttcta taattttgct attttcaaaa aaatcatgaa
     7741 ctggtgatgg taaaattgtt ataaaatcag aatttgtggt tgatagcaac aaaaaatccc
     7801 atgaaccaga cattatatat ctttttaact taacattttc atcagtaaat ttttgcatta
     7861 gcttatgatg tatcataaaa gtaggattaa atattgcaag taattgctca ttcaaatcgt
     7921 tccaatctat cttatccttc ttagcaagtg gatgatttat atttaaaaat gctgtaagtt
     7981 catcttcttg aagcacatgt tcagttacta cacttttatc aattttatgt ggatgtaaca
     8041 gtactgcata atctagttcc tgcaagataa gcattctatt taagtcatat gcacccttct
     8101 ctactatatc aaattctata tctggattat ttgcaactaa ctgtgcaact acatctgaaa
     8161 atgctattcc aagtataagt ggaggtattc ctattctaac ttttccttta aattgaactg
     8221 aatcttctct taaatcttcc atcatatttt tgtattcatt tataatatgt tctgcattta
     8281 cataaaatct ttctcctgct ggtgttaacc catttaacct acctttatac ctttcaaata
     8341 aatatacatc ttcactttct tcaaaatttt ttataatttg acttaatgct ggttgagata
     8401 tgtgaagtgt cttagatgct attgataaat taaaaccaga tttcactatt tcaacaaaat
     8461 atttcaactg ttttatatcc attttaaacc cccttcccca aaagtataat ttagattata
     8521 ttttttcttc taatgatatt ttataggaaa atcaacataa tttcattaat attttaacaa
     8581 aatatttttc taaatctact ataaattttt atcctataaa tttttcttat acactatata
     8641 aaaaattaga cttagacaaa atgctttttt cgccgtaatc tataattaga aacaggaaaa
     8701 cctaaagcat taggaataaa agtaattgaa atacttaaaa acaagcttgt attaaaaaag
     8761 tactttatta tagtgttccc taatttcttt attcagaaaa tttttaatta tcattcaaga
     8821 aaggtaacta ttagatatga aagatgtagt aatcgtatca gcagtcagaa cacctatagg
     8881 aagttttgga ggtgtattta aaaatacttc agctgttcaa cttggaacta tcgctgttaa
     8941 agaagcgata tcacgtgtag gacttaattt atctgaaata gatgaagtaa taattggaaa
     9001 tgttcttcaa acagggcttg gacagaatgt tgctaggcaa attgctataa attcaggtat
     9061 accaaattct gttccaagct acacagtaaa taaattatgt ggttcagggc ttaaaagtgt
     9121 tcagctagct gcccaatcaa tcacatctgg tgaaaatgat gtggtaattg caggtggtac
     9181 tgaaaatatg agccaagctc cttatatcgt tccaactgct cgctttggta gtaaaatggg
     9241 tgacataacc atggtagata gtatgctaac agatggcctt attgatgctt tcaatcagta
     9301 tcatatggga ataactgcag aaaatattgc aactaaattt gaatttacta gagaaatgca
     9361 agataaattg gcattagaaa gtcaaaataa ggctgagaat gctataaaaa acaaccgctt
     9421 taaagaagaa atcgttccag tagatgtttt aatacgtcga ggaaaaatag aaactattga
     9481 taaagatgaa tatcctaaat taggcatgac atttgaaggt ttagctaaac ttaaacctgc
     9541 attcaaaaaa gatggtactg taacagctgg aaatgcctca ggtattaatg atggtgcggc
     9601 tatgttaata ctaatgagcc aacaaaaagc agacgaacta ggtattagac cacttgctaa
     9661 aataaaatcc tatgcttcag ctggtgttga acctgaagtc atgggtactg gtccaatccc
     9721 tgcaactaga aaagcactga aaaaagctgg acttagcatc aatgatatag acttaataga
     9781 agcaaacgaa gcgtttgctg cccaagcatt agctgttaaa aatgaacttc aaatagattc
     9841 atcaaagtta aatgttaatg gtggtgcaat agcattagga catccaatag gagcaagtgg
     9901 agctagaata cttgttacct taatttatga aatgcaaaaa cgtaaagttg aaactggact
     9961 tgcaacactt tgtattggtg gtggtcaagg aatatcaatg gtggtttcac gataggagga
    10021 aatatgaata aaatagttag cattgatgaa gctctatctc atgtaaaaga tggagataca
    10081 ataatggttg gtggattcat ggccaatggt tcaccagaaa atttaattga ttatttatgt
    10141 agtaaaaata taaaagattt gacactaatt tgcaatgata ctggctttat tgacaaagga
    10201 gttggcaaaa tggttgtaaa taagcaattt aaaaagataa ttgcttctca tgtaggatta
    10261 aataaagaaa ctggaagaca aatgaacaac aatgaaacag atgttgagtt agttcctcag
    10321 ggaactcttg ctgaacaaat acgtgcagct ggttatggtc taggaggtat actcacaccg
    10381 actggtattg gtactcttgt tgaggaggga aagcaaaaaa ttgttgtcga tagcaaagaa
    10441 taccttcttg aaaaaccaat ttatgcagat gttgctcttc tatttgcatc aaaggtcgat
    10501 aaagcaggta atctagttta taaaggctct atgaacaatt ttaataattt aatggcatct
    10561 gctgccaaaa ttacaatagt tgaagcagat gaaatcgttg aaattggtga cattgaccca
    10621 aatgaagtta atacaccagg tatttttgtg aattacatcg ttgaaggagg taatttataa
    10681 tggataaatt agaaatgcaa gaatatattg caaatagagt atctaaagaa ttaaaagacg
    10741 gagccgttgt aaaccttgga ataggtctac caaccaaagt agcaaattat attccggata
    10801 atgtaaacgt tatcttacaa tctgaaaatg gctttttagg tttaggttca gccgaagatg
    10861 gaaattcaag tgatgaaact attgtaaatg caggtggtca accagttact atacttcctg
    10921 gaggatgttt ctttgatagt gctacttctt ttggaataat cagaggaggt catgttgata
    10981 taactattct tggcgcactt caggttgaca aatatggcaa tatagcaaac tatatgattc
    11041 caggtaagat ggttccaggt atgggtggag ctatggacct agtaactgga gctaaaaaag
    11101 ttattgtagc aatggaacat acttcaaaag gttctgcaaa aatattaaat aattgtactc
    11161 ttccacttac tgcaacaaat gctgtagatt tgatagttac tgaaatggga gttatggaag
    11221 taacatcaga tggaatttta cttaaagaaa taaatccagc tttcacatta gatgatgtta
    11281 tttctgctac tgaagctcct ttaatattgt cagattcatt aaatggaaaa atatctgttg
    11341 tttaaaataa ttaaaactag aaaataaaaa tctcttagac gtattacata taaatttgta
    11401 ttgcttaaaa tattaatata aaaataaaat aaaattggag gaaatgaaaa tggttaaaga
    11461 taaagttgta ttcgtaacag gagctgcaag tggaataggt aaacaaatag ctgaatcgtt
    11521 cttaaaaaat ggtgctaaag taatgttctc tgatataaat caagaagctt taaataatac
    11581 cataacagag ttacaaaaaa aaggttatga ttgcatgagt gtaaagtgtg atgtaactaa
    11641 ggaagaagaa attaaccacg ctatagataa aactgttgaa aagtatggca gattagatat
    11701 attaataaac aatgctggac ttcaacatgt ttctatgata gaagatttcc caactgataa
    11761 atttgaattt atgataaaaa taatgctgac agcacctttt atagctacta aaaaagcatt
    11821 tcctataatg aaaaaacaag gctttggtcg tataataaat atggcctcaa tcaatggtgt
    11881 aataggattt gctggaaagg ctgcttataa ctcagcaaaa catggactta ttggactaac
    11941 aaaagttgct gctcttgaag ctgccgattc tggtataaca gttaatgctt tatgccctgg
    12001 atatgtagat actccacttg tcagaggtca gtttaatgac ctttcaaaaa ctcgtaatat
    12061 accattagaa aatgtcttag aggaagtttt gtacccactt gtaccacaaa aacgcctatt
    12121 agatgttcaa gaaattgccg actatgctat gttcctagca agtgataatg ctaagggtat
    12181 tacaggacaa gcttgcttac tagatggagg atatactgca caatagttat tatatttagg
    12241 gcttgaagaa agtattcttc aggtccttct actaaggagg gatatttatg agtattatcg
    12301 caccaattgg tataattatt ggtatcatag ctattatata tttttcagtt aaagaaattc
    12361 atataactat agctgctcca atagcaactt taatagttgt tctcttaaat aaaatggatg
    12421 ttgtaacatc aatgctaggt gctgacaaaa acaattatat gggtgcactt ggaaactata
    12481 taatgagtta ttttgcaata ttcttattag gctcaatact tgctaaattt atggaagaaa
    12541 gtggagccac tgtatcaata gctgatttta ttttaaataa agttggctct aatcatcctt
    12601 atagagtttt agttgctata tttatagtaa gttttatatt aacgtatggt ggtataagct
    12661 tatttgtagt tatgtttgca gtcattccac ttgcaagaac tctatttaaa aaacttgata
    12721 tttcttggaa ccttattcag ataccacttt ggcttggtat tgcaacaatt actatgacta
    12781 taattcctgg aacaccagcc attcaaaatg ttatacctat tcaatatcta aatacgtctc
    12841 ttactgcagc tactatacca agtattgtag gtagtatagg ttgtgctacg tttggacttg
    12901 tctatatgaa atatgctcta aacaaaagta ttagaaaagg tgaaacttac gcaacttaca
    12961 cttcaaaatt agaagaagaa tctttagata gagaattacc aggatttttt gcaagtattt
    13021 ttcctctatt agcactcgta attatagcta taactggaag tacttttggt tcagaattta
    13081 tgaaaaaaaa tgtaatttac atagctttaa tagttgcaat tcttttatct gcttttttat
    13141 tccataaatt tctatcatct aagatacaga cattgagcat aggtgctagt ggttctatag
    13201 gtcctatatt tgctacatct tctgcagttg catttggagc agtaataatg gctgcacctg
    13261 gttttgatgt atttagcaaa cttataatgt caataccagg tagtccactt ataagtctta
    13321 ctgttttgac ttcaacaatg tcagcaataa cagggtcctc ttctggtgca ttaggaatag
    13381 taatgccaaa ctttgcagat tattatttaa gtactggtct aaatccagaa ttaattcacc
    13441 gtgttgctac aatagcctca aatatactaa ctatagttcc acaaagtggt gtttttctta
    13501 cattcttaag cttatctaag ttaacacata aaactggatt taaagaaact ttcattgtag
    13561 ttgcagttgg ttcactaata gcagaaatta ttgtaattac attaggatta ctaatgtatt
    13621 aatataaaac aaattaaaat tcataatatg aaaggtggtg tctcaaagtg aactttttac
    13681 ttaatgagac actctttttg tatgaaatca aatatatttt aatcatttca acaataaaaa
    13741 agaacctttc tctattttga gacaaaccct ttttattcct taaagattta aacaattata
    13801 atttgttact aatacgaaaa catctattga ataaatttaa ccaaataaaa aaaaatttat
    13861 cataactata aatttacgtt atggaattat tataaaaata ttattttctc taagtaagta
    13921 attccatact atacaggctt ttaatacaaa ttttcaaaca cactgaaata tacctattta
    13981 ttccatatat atctttttta catatcttta cagtaaaaaa agtcttaatc ttatgttagt
    14041 atagattcac gggtaccccc tacactaaag gttacaaatc agtaacattt ttagaatttt
    14101 tagattaaga ggtgtttaat gatgagaaga aatataccaa caaaattaaa aactatatta
    14161 gctttaggag tgttaacttt agcaacagct tcaagtgctg ttactgcaag tgcttcatca
    14221 cttgaaaata atgtaaatat aagtaacaaa agtttagtat atagtaatac taaatacaag
    14281 ttttctgcaa aaccaaattg ttctgtaaaa ccagaactta aacctgataa agataactct
    14341 tgtaaggata agaatcataa tgacaagaat tgcaacaaca ctaataaacc tgaggacaac
    14401 aataattcag actctactaa taagcctgat aacaataaac cagagtctaa taaacctgag
    14461 gacaacaata attcaggctc tactaataaa cctgataaca ataaaccaga gtctaataaa
    14521 cctgaagaca acaataactc aggctctact gataaaccta ataacaataa accagagtct
    14581 aataaacctg aagacaacaa taattcagac tctactaaca aacctgatga taataacaac
    14641 tcaggctcta caagtgaaaa tttttcagct taccaaaaag aagttgtcga cttagtaaat
    14701 atagaaagag ctaaagctgg attaaatcct cttactcttg attcttctat atcaaatgtt
    14761 gctacaaaaa aatctcaaga tatgattgat aacaactatt tttcacacaa ttctccaact
    14821 tatggttctc catttgacat gttgaaaaaa tttggaataa gctataagac agcaggtgaa
    14881 aatatagcta tgggtcaaaa aactcctaaa gaagttgtaa acgcatggat gaattctgaa
    14941 ggtcacagaa aaaatataat gaatccaaat ttcagtaaaa taggtgttgg agtagcacaa
    15001 aaaagtggtg gttcaattta ttggacacaa atctttgtag gataaaatac tctttttcca
    15061 tgcatatcaa aagagtatct ctaagtgata aaaaacacaa atgagatact cttttgtatc
    15121 tattttaaaa ttatcaaatc caaatataca tactataaat attatttgtt ctcttaattt
    15181 tagtgtgtag aacaatatat aaaaagtatt ggcaagatga aaatggaaaa cttaaaaaga
    15241 tatttaatag gtaaaaacag gatcttaata tcttcataaa aaaaggctgt ctcaaaatag
    15301 agataaggcc tttttcattg ttgaaatgat taaaatatat ttgatttcat acaaaaagag
    15361 tgcctcatga actaaaaagc tcactttgag acactctctt ttatcaattc tatataagca
    15421 ttataatact ttaatattat tcagctaatt ttttgtatcc ttcatatctt tctttagcat
    15481 cagcttcagc tttagcaaat aatccatcag cttcctctgg gaattgttta gctatagcag
    15541 aatatcttac ttgtgcaagt atgaaatctt tgaagcttgc agttggttct tttgaatcta
    15601 aagtgaatgg gtttttacct tcagctttta cttctggatt gaatctgtat aaatgccagt
    15661 atccgcaagc aacagcttgt gcttcattag ccacagttct tcccatacct tcttttaatc
    15721 catggtttat acatggagca taagctatta ttagagatgg cccatcatgt ttttcagctt
    15781 ctaaaacggc tttgataaat tgatttttat cagcccccat agcaacttga gctacatata
    15841 cgtttccata tgtcatagcc atcattccta aatctttctt tctagatttt ttacctgaag
    15901 cagcaaactt agccatagca gcagctggag tagctttaga agcttgacct cctgtatttg
    15961 aatatacttc tgtatcaaat acaagtacgt ttacatcttc accagatgca agaacatggt
    16021 ctaatccacc gtaaccaata tcatatgccc aaccgtctcc acctagtatc cattgagatc
    16081 tcttaactag ataatctttt ctatctttta tagcttttac taattcttta gctttttcat
    16141 ctgtacaatc acaagctggt ttttctaata attcaacaac cttagcactt gctactttag
    16201 aagcttctcc atcatttcta gaatctaacc actctgtaaa tactgcttta gcatcttcac
    16261 agatatccat agaaactaac tctgtcatag catctactat tttatttctt atttgcttaa
    16321 cagctgtgta cataccaaat ccatattcag cattatcctc gaataaagaa tttgcccaag
    16381 atggaccttt accttcatgg ttacaagtat aaggaattga tggtgcagaa gctccccata
    16441 tagaagaaca tccagtagca ttagctatca tcattctatc tccatataat tgtgtaacta
    16501 ctttaatgta cgcagtttct ccacatccag cacatgctcc tgaaaactcc attaatggtt
    16561 gtctaaattg acttccttta acagtatttg caggcataac atcaactttt ggagctactt
    16621 catcaggatt tactgcaaat gcccaattat ctaactctgt atcaagttga gtatctattg
    16681 gcttcataac taaagctttt tctttagctg gacatatgtc agcacagttt ccacatcctg
    16741 tacagtcaag tggactaact tgaattctat attttaatcc ttctaatcct ttacctaatg
    16801 cttttttagc ttcaaatcct tctggagcat tagctaattc ttcttcagta actaatactg
    16861 gtcttataca tgcatgagga catataaaag aacattggtt acattgaata cagttatcta
    16921 ttatccattc aggaacatca acagctatac cacgtttttc ataagctgca gtaccacaag
    16981 ggaatgttcc atcttctata ccattgaatg tacttactgg taagttgttt ccttcttgtg
    17041 cagtcattgg tcttagtata ttttttataa attctggttc tttaacttct cctttatctg
    17101 catcaacagc attttcccaa gaagctggaa cttcaacttt aactaaagca tttattcctg
    17161 catcaacagc agcatagttc atattaacta ttttttcacc ttttttacca taagctttaa
    17221 ctatagaatc ttttaagtat ttaacagcat cttcttctgg tattatttca gctaatttaa
    17281 agaatgcaga ttgcattatc atgtttattc tgtttccaag acctatttct tgtcctagtt
    17341 taacagcatt aactgtatag aattttatat catttttagc tatatattgt ttcatatgag
    17401 ctggtaaatg ttcttcaagt cctgcttggt cccatatagt gttaagtaca aatgtaccac
    17461 cttttttaag accttttaat aaatcatatt gataaacata tgattgttta tgacaggcaa
    17521 tataatcagc ttcatctatt aaataagtag ctcttattgg agtatcacca aatcttaagt
    17581 gagacatagt tataccacca gattttttag agtcataatc aaagtaagct tgagcatatt
    17641 tttcagtatg gtcaccgatt attttgatag cttgtttatt agctccaaca gtaccgtcag
    17701 aacctaatcc ccagaactta catcttatag ttcctttaga agctattttt attggctcag
    17761 atggagttaa agaagtatta gttacatcat caactatacc tactgtaaat ccatttttag
    17821 gttgttctga aactaagtta tcaaaaacag tttttatatc tgaaggagta gtatcttttg
    17881 aacctaatcc gtatcttcca cctattatca taggagcatt ttcttttcca tagaatacat
    17941 cacgtacatc taagtataat ggttcaccag ttgaacctgg ctctttagtt ctatcaagta
    18001 cacatattct ttcaacagta gatggcatag catctaagaa atgtttcatt gagaatggtc
    18061 tatataaatg aactttaact aaaccatatt tttctccctt agcatttaag taatctatag
    18121 tttcttctat tgtttctgtt actgaaccca tagctattaa tacgtattta gcatcttctg
    18181 ctccatagta atcaaataaa ctatgttttc tacctgttaa attactcatt ttttccatgt
    18241 atttttcaac tatacctact atattttgat agtatttatt agaagcttct ctagtttgga
    18301 aatatatatc tgggttttga gcagtaccac gagcaactgg atgatttgga gataaagcat
    18361 tatctctaaa tgcttgaact gcttcaaagt ttaataaact tgcataatct tcattttcta
    18421 gtaattcaac tttttggatt tcatgtgaag ttctgaatcc atcaaagaaa tgtataaatg
    18481 gtaatctacc ttctattgca gctaaatgtg caacaggtgc tatatcagct acttcttgaa
    18541 ctgaaccaga agctaataat acacatccag tttgtctagc agccataacg tcttggtgat
    18601 ccccaaatat tgataacgct tgagatgcca aagcacgagc acttacatgg aatactcctg
    18661 gtaataactc tcctgcaact ttatacatgt taggtatcat taataataaa ccttgagatg
    18721 ctgtaaaagt agaagttaaa gctcctcctt gtaaagaacc gtggaatgtt cctgatgcac
    18781 ctgcttctga ctgcatttca acaacattaa ctgtttgtcc aaatatattt tttcttcctt
    18841 gtgatgccca ctcatcaact acttcagcca tagttgaaga tggtgtgatt ggatatatag
    18901 ctgctacatc tgtaaacgca taggcaacat gagctgcagc tgtatttcca tcaagtgttt
    18961 tcataaactt agccataata tgtatcctcc ttgatagcta caattgtact tgtttataac
    19021 aagtctattt ttttatattt acatataatt tacacaacaa aaatataaac acacatatag
    19081 tcgcgcaaat actctatctt ctaatataat actacagatt agataaaatt cctttgaaaa
    19141 ttgtaaatta attacatatt taagtagatt ttttttaaaa ttaaatatgt taagt
//
LOCUS       DAMJVX010000063        18995 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMJVX010000063 DAMJVX010000000
VERSION     DAMJVX010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18995
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.066"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(151..438)
                     /locus_tag="PC407_001963"
     CDS             complement(151..438)
                     /locus_tag="PC407_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDF2497409.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            complement(516..941)
                     /gene="ruvX"
                     /locus_tag="PC407_001964"
     CDS             complement(516..941)
                     /gene="ruvX"
                     /locus_tag="PC407_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDF2497410.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            complement(1147..3318)
                     /locus_tag="PC407_001965"
     CDS             complement(1147..3318)
                     /locus_tag="PC407_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497411.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYGTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNVGYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIININMIIFEKNN"
     gene            complement(3328..3585)
                     /locus_tag="PC407_001966"
     CDS             complement(3328..3585)
                     /locus_tag="PC407_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDF2497412.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            complement(3610..6249)
                     /gene="alaS"
                     /locus_tag="PC407_001967"
     CDS             complement(3610..6249)
                     /gene="alaS"
                     /locus_tag="PC407_001967"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDF2497413.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     misc_binding    complement(6317..6555)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6698..7777)
                     /gene="mnmA"
                     /locus_tag="PC407_001968"
     CDS             complement(6698..7777)
                     /gene="mnmA"
                     /locus_tag="PC407_001968"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDF2497414.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     gene            complement(8066..8506)
                     /gene="nifU"
                     /locus_tag="PC407_001969"
     CDS             complement(8066..8506)
                     /gene="nifU"
                     /locus_tag="PC407_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HDF2497415.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            complement(8560..9753)
                     /gene="nifS"
                     /locus_tag="PC407_001970"
     CDS             complement(8560..9753)
                     /gene="nifS"
                     /locus_tag="PC407_001970"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HDF2497416.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGRVDLRQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            complement(9771..10217)
                     /locus_tag="PC407_001971"
     CDS             complement(9771..10217)
                     /locus_tag="PC407_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2497417.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            complement(10326..11096)
                     /locus_tag="PC407_001972"
     CDS             complement(10326..11096)
                     /locus_tag="PC407_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2497418.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDTLAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            complement(11127..12392)
                     /locus_tag="PC407_001973"
     CDS             complement(11127..12392)
                     /locus_tag="PC407_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDF2497419.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            complement(12499..13284)
                     /gene="codY"
                     /locus_tag="PC407_001974"
     CDS             complement(12499..13284)
                     /gene="codY"
                     /locus_tag="PC407_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDF2497420.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            complement(13449..15536)
                     /gene="topA"
                     /locus_tag="PC407_001975"
     CDS             complement(13449..15536)
                     /gene="topA"
                     /locus_tag="PC407_001975"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDF2497421.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            complement(15569..16669)
                     /gene="dprA"
                     /locus_tag="PC407_001976"
     CDS             complement(15569..16669)
                     /gene="dprA"
                     /locus_tag="PC407_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDF2497422.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGYAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            complement(16688..18289)
                     /locus_tag="PC407_001977"
     CDS             complement(16688..18289)
                     /locus_tag="PC407_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDF2497423.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLLVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVSIMSFLDDKSRKENGELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISIEEMIEVSKV
                     YSILGMINESKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            complement(18320..18664)
                     /locus_tag="PC407_001978"
     CDS             complement(18320..18664)
                     /locus_tag="PC407_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDF2497424.1"
                     /translation="MNNKEKGDFGEEIAVNYLLSKGAKILEKNYRLRIGEVDIIANIG
                     DEVVFVEVKSRSNIKYGYPCESVDFKKRKKIIGVAKYYIIKNNLNNTPIRFDIIEVYL
                     LEKRINHIMNAF"
BASE COUNT         5693 a         3590 c         1916 g         7796 t
ORIGIN      
        1 tattattcac caaaattcat aaattttttg gtatttttaa taaaaaacaa aaaaacagat
       61 atatactata aatgctttta caattaataa ggtttaatta aagcaaatat aataaatatc
      121 tgatttttat atacaaactt aattacttat ctagttaagt ccatttgatt catcttcgtc
      181 gtaatttaaa ccttcttctt ccattatagc agtatacaca gctacaacag tttcatactc
      241 ttcatcactt tccaaaggta ttaatatttc accttcttca tcctcatcaa ttctaaagat
      301 taaagcttct tcagcttctt catcatctaa aggcattaat attgcatact cttttccttc
      361 tgcttctaat gttaatatta tttcaaattg gctttcaacg ccattttcgt ctattaaatt
      421 tattatattt tcttccataa tgtcattcac ctcaaattta tttttatttt tattatttat
      481 attatatatc ataagtattc aattttatag ttcttttatg aatttatttt aaaatctaaa
      541 tatccttgca aaattattac tgctgccaac atatctataa ccttttttct tttttgtcta
      601 ctaacatctg cttctaataa agacctctct gccgctactg tagaaagcct ctcatcccaa
      661 aactctacat caatacctgt ttctgctttt accaattcac aaaacttgat tactttttct
      721 ccttgtggac ctaaagttcc attcatattt tttggtaatc ctgatacaat tttatttact
      781 tgtttttctt ttattattgc ttttaactct tcaatatctt ttttcttgcc aactcttttt
      841 attgttttta caccttgtgc agttaatccc atcaaatcac tcacagcaac tccaatagtt
      901 ttatcaccaa catcaagacc cattattctt ccatctaaca tagttcctcc tttatctatt
      961 tatattctat agattaaata catattctga acctctcata actaaggtca caggcttcct
     1021 gcttcataga attttgcata atcaatcata aaaattgcaa tatgattttt tatgtaagca
     1081 tccagtttac attaataatc atattgcatt ttttatttaa atcctatatg aacagtaaaa
     1141 aattttttag ttgttttttt caaatattat catatttata tttattatta ttagtggtat
     1201 aagtatatat atagatgctg tagatgcagc aactattcca gaatcattaa ctaaaagagt
     1261 aattatacaa ccaaccatag atgcaataaa ccctttaaac aagattggaa aattattcat
     1321 tatctttcta aagtgtctag atggtttaaa tataagtact gcaattactc caattcctac
     1381 aagtaaaata tttacccata cacttgtctg tgctagttgc atattcattt gaatttttct
     1441 tccaaatgtt tgtaatatct ctcctggtcc atttaggaaa attctctgta caaacatacc
     1501 taaatgagat tcactacctg aaagtatatc taaagctgca aatgcaaaaa cgacaactac
     1561 agctgaaaca gctaataata cagctttctt aaaatctatt ttaatgtcat atataagcat
     1621 tataaacaac aaatatgcta cacattctga tatagctcca ccaacatttg cacccattga
     1681 aggatatgca ctagttataa gtattattaa cgaaaaaatt acaacaagta atttagatac
     1741 cttcttataa tgtaataata ctgctaagcc aaatactgca cttgctatag ttacaccttc
     1801 atattcattt cctacaccat agtacctagc tcctatgaca gcatcataac tcattatgtt
     1861 attttgcatt aagtatgttc caaatacaga atctataact atgactaaaa tagtaagtgt
     1921 tgcaaaaaat cccatgtgct ttatatcatt gtccttaaat aattttctac ctaacaagta
     1981 taatattagc gtagtcgcaa atattcccaa agatattgaa attggagttt taaaattcat
     2041 caaaggagct aacataaatg atagtggcat tattatgcct aattttataa actccttaag
     2101 tactttgaat acacgctctt tatgtggcat ataatttcta aagaaaatcg caaacatggc
     2161 aattacccaa gaagcagata ttacgcctac aaagccattt ataatagttg accttattga
     2221 agatatagac actatccttt cataaagtga ttttagatag ccaacattgt cctctttatt
     2281 tactaaagta taacttctac ctaccatctc tttattttca agaccaaatg tacctagtat
     2341 atctactcca acatctaaat tggcaacaat tccagccttt cttgttgtgg cagattccag
     2401 tagacctttc ccttctccac taaattttat tattggagaa agtcttctct tattcttata
     2461 atctaggtca cttggaaatg cacttgctat ataaacagtg tcattttctc caaccataga
     2521 aaatacttgt tcaagatatt tatttatatt ttttacaata tttttcttca ttgtaccata
     2581 agtattatca tttagattta atctatacat atctaatcta tatgtatctc caagttctac
     2641 aaatatagca tcactatttt tataggattt tttagtttca tctagtaatt tatcgtagtc
     2701 tgtagatatt ccaaatggca ttttagaatc tttcttatta atattatcaa tattacctga
     2761 atcaatcctt ccatattggt ccatagctac taaacataag tttctatttt taactaattg
     2821 attattttca attatatcag catttccaat tacagatgtc tttaaaccat tttctgataa
     2881 agtttttcct aatgacccta aatttgctcc atattctcca tactcaagat tttcatttat
     2941 agatctatta atgcctaaat catttatttc tttagctttt tgacctgttg cgacttcaaa
     3001 agctttagca gtatcttggt ctactttttg aaaattgata tagtcttcag tagttacact
     3061 agctcttgtt cctgcaccca tacttgcata agaccttcta tcatcagtac cttgatcccc
     3121 tcttatattc atgagagcta catagcctct cttttctatc tcatttttca agattggaat
     3181 attcatcata cttccaagat tggtcctatt catatcaata aatatgacct tgcctttttc
     3241 ttcagcaaaa ccatagctag cagttgatac aaaaatcata atgactattg caaacaacga
     3301 cataatcttt tttctcattt atttgaccta ctttctattc agatagtgcg tgattacttc
     3361 ttcaagtatc tcatctcttt caaatttttt aattatagct cttgcatttt tatgactagt
     3421 tatatagcta gaatcaccag aaaggagata ccctataatt tgatttatag ggttatatcc
     3481 tttttcaact aatgctttat atacaaaatc aatagtatct cctacactca tcctatcttc
     3541 tggtattcct tcaaatttca tagtgtagtc taaatctttt tccaaactaa aaacctcctt
     3601 gagtgaatgt tatttaactt gtgtcttaat aacctcactt gcatagctta aagcttggtc
     3661 aaccttagat acatcactag ctcctgcttg tgccatatta ggtcttcctc caccttttcc
     3721 tccagctatt tttgcaactt ctctaacaat attacctgaa tgtattccct tatctaatac
     3781 atctttagtg gcagtaacta caaagttaac tttatcatct actacatttg caagtactac
     3841 tacaccagaa ccaagcttat ctcttagatt atctgctgtt tctcttagtg tatccatatc
     3901 cataccttca aatttattag ttatcaaatt aactccattt acttcaactt tagaatcaaa
     3961 aatagaatca actgattgta aactcatctt agctttcata tcatgcaatt ctttagataa
     4021 gtttttattt tcttctaata aagaacttat tctttgaatt aaattatctt ctttagattt
     4081 taagtttaca caaacttcag agataattcc atctctttct tttaggtatt cataaacagc
     4141 ttttcctgtt attgcttcta ttcttcttac acctgctgct actccacctt cagataatat
     4201 tttgaacatt cctacttgag aagtatttgt caagtgagtt cctccacaaa gttctgtaga
     4261 ataatctccc atagaaacta ctcttacctc atcaccatat ttttcaccaa atagtgctgt
     4321 agctcctttt tctttagctt cctttatatt cataatatca cattttatgt ctaaagatga
     4381 taaaataaca ttatttactt tttcttctat tacttttaat tcttcatttg atatcgcttc
     4441 aaagtgagta atatcaaatc taagtctttc tggagttact aaagaaccag cttgattaac
     4501 atgttctcct aatacttctc ttaatgcctt atgaagtaaa tgtgtagcac tgtgatttct
     4561 tgcagctgac attctagttt ctctatctac gagtgtcttt actttgtccc cattacttat
     4621 tcttcctgat tttattatac ctatatgttt tatactatta ttagcacctt ttttagtatt
     4681 taatacttct aaaactaagt tttcattagt tatcaatcca caatctccaa cttgtcctcc
     4741 accttcagga tagaaagtag tgttatctag tactatagat accttatcgc cttcttctgc
     4801 actttgaact agctcatcat ctttaactat agcttcaata gttccttcac cataaatttc
     4861 actataccca ttaaatgtac tgtcaactgt agattctaat tttgatagag ggtcttcctt
     4921 ccaactttct ccatccatat ttcctctagc atttctagct ctttcttttt gcttttccat
     4981 ttcttcatta aaagcttctt catctacaga tagatgttct tcttctaata tttctttagt
     5041 taagtctata gggaagccat aagtatcata tagtttaaaa gcttcttgac cacttaggac
     5101 agtctttcct tcatttttta atacttctat ataagagttc aatatttctg ttccttggtc
     5161 tatagtttca ttgaattttt cttcttctat tccaactact tttttaatat aactttcttt
     5221 ttcaactaaa actggatatg ctttatcatt tacctttata acttcatcta ttagtttctg
     5281 taaaaataat tcttttactc ctaataactt accatgacga gcagcacgtc taaggagtct
     5341 tctaagaaca tatcctcttc cttcatttga tggaagcact ccatcactaa ctaagaatgt
     5401 tacagctctt atgtggtcag ttattattct tattgatata tcattttttg gattctctcc
     5461 atattttact cctgttaact tctcaactgc ttctagtata gatttaattg tatcaacttc
     5521 aaagatagtg tctacgcctt gcataataca tcccattctt tcaagaccca tacctgtatc
     5581 tatattcttg ttttctaata gactataatt tccttcttca tctctatcaa attgagtaaa
     5641 tacatggttc caaaattcta aatacctatc acaatcacag cctggtttac agtctggatt
     5701 gtcacatcca tattcttctc ctctatcaaa atatatttct gaacaaggac cacaaggacc
     5761 tgtacctatt tcccagaaat tatcatcttt tccaagtcta accattcttt cttctgggaa
     5821 attcatttct tttgcccata tatcataaga atcatcatct tcttcatata cagtcaccca
     5881 tatcttatct tctggtatat ttagccattc tgtaacaaat tcccatcccc atttgattga
     5941 ctctctttta aagtaatctc caaatgagaa gttacccatc atttcaaaga atgtagcatg
     6001 tcttgcagtt atacctacat tttctatatc tcctgttctt atacattttt gagatgtaca
     6061 cattctcaca cttggaggta cttctacacc tgaaaagtag tttttcaaag gtgccatacc
     6121 tgagtttata agtaacaaac tcttatcatt gtttggtact aaagaataac tatttgctac
     6181 ataatgacct tttgactcaa aaaattctaa aaacttactt cttatttcat ttaatcccat
     6241 cttttccata tcatgttcct cctaataaat tttatttttt cgtttaaata aaaaacctcg
     6301 tccctataaa taaatttata ggggcgagga caatcgcggt accaccctaa ttatcataca
     6361 aagtatgata tcttcggaaa accttagcta taacgtaact accgtcaatc tctacttaaa
     6421 tcttctttat aaaagataaa ttgttcaaga ttgctactaa aaggctgctt caaatatcat
     6481 attctaaggg cttccagcat ttccctctct ctgtaagaca tcaaatattt tactattcct
     6541 tatcactgta tttgtttttt aattaatata ctaattatat tactaaattt tacaaaatta
     6601 atcaatacta tattttaata tacaataaat ttattttata aactttattt actcttaata
     6661 tactttattg cttatttttt taatttttta gatgttttta taaacttttc tcgattatac
     6721 ctccacctac tacaatatct ccatcataca ttactacaga ttgaccttta gtaatagctc
     6781 tttgagcttc atcaaaaact attttaatag tatcttctcc tactctatga atagtagctg
     6841 gtgatggttt tgctgaatat cttattttgg cttgaactct taagggttct tctaacgtat
     6901 ctattgatat aaagttaaca tctttagcaa taagttcctt tttaaataaa tcttcattgt
     6961 ctccaagaac tatggtatta tttattggat ttatgtctat tacaaacatt ggttttccaa
     7021 aagtaattcc aagccctttt ctttggccta tagtataata taatataccc ttatgttttc
     7081 caagtatatt gcctttagta tctacaaaaa aaccttcttc aatacgcttt ttagaatgtt
     7141 tttttacata atttgcatag tcattatctt taacaaaaca aatttcttga ctatcaggct
     7201 tattgtgtac tgccatcccc atttccttag caatttctct tactctatct ttttcatagt
     7261 cacctattgg caataaagta tgctctagtt gctcttgtgt aagattataa agtgcataag
     7321 tttggtcttt tttatctgta acagattttt ttagcaaata tcttcctgta ctttcatcct
     7381 tttctatctt tgcatagtga cctgtagcta cataatcaca acctatttgt ctagccttct
     7441 tataaaaatc atcaaatttt atatgtttat tacaagctat acagggatta ggagtttttc
     7501 cttccaaata ttcatctata aaataatcta ttactttttc tttaaacact tctctaaaat
     7561 ttaaaacata aaatggtata cctattttat ttgctaccct tctagcatct tcaacagcag
     7621 ataaagagca acaaccacct tcaatttcaa caacatcatc atcttgccac aacttcatag
     7681 taacaccaat gacatcatat ccttgttgtt ttaaaagata tgcagctaca gagctatcaa
     7741 ctcctccact catacctatc attacttttt tattcataaa caaatttcac ctcatctttt
     7801 gcattactta ttattatacc atatagagca agtattgtac ttatttaaaa tatagcttgt
     7861 tctattttct aaaaaaataa tcatctttat tatttgaatc ttatggatta ttttgttaac
     7921 taattaacct ctaaatcaag ttaaaatact taatttaggc aataaaaaat agccatttta
     7981 aaaacagcta ttttttatgt cctaattata tcattagtac ttctttttaa tcattgtaaa
     8041 cttctcataa cacaatcact tatttttatt cttcttcttc aacatcatgg tcatgagcat
     8101 catctataac atatccatcc aattctggta tatggatatt gttcttttga gcataatcta
     8161 taagagctgc ttttacagct tgttcagcta aaactgaaca atgcatcttt actggtggta
     8221 aaccatccaa agcttctgca acagctttat ttgtaagttc taatgcatct tttatagttt
     8281 ttccttttat catttcagtt gccatagaag aacttgctat agctgaacca cagccaaatg
     8341 ttttaaactt aacatccttt attacatctc catctatgtc taaaaatatc ttcattatat
     8401 ctccacatgt agggtttcca acttcaccta caccacttgc attatcaatt tctcccatat
     8461 ttcttggatt cataaaatgt tccataactt tatcactata ttgcattatc ttttcctcct
     8521 actcaattta tgtctatttt ttatgcttta tctttctgtc tattttacta aaccttgttt
     8581 cttcgcatca tcataaagtg gtgacatgct tctaagtctt tctattactt ttggtaagtt
     8641 ctctagtata taatctatat catcatctgt tgtaaaatct ccaactgtaa gtcttaatga
     8701 accatgtgct atttcatgtg gtaaacctat tgcaagaagt acatgtgaag ggtctaatga
     8761 accagaagta catgcagaac cacttgaacc agcaattcct aacatatcta ataataaaag
     8821 tattccttca ccttctataa attggaatgc aaagtttgca tttcctggca atctattttc
     8881 taagctacca tttactcttg tgtaaggtat tctttctaac acaccatcta ttaacttatt
     8941 tctaagacga gttaaagttt ctacatgatt ttgcatattt tccttagcta attctgctgc
     9001 ttttccatat ccaactattg caggtatatt ttctgtacca gctcttttgc ttttttcttg
     9061 tgctccacca tggacaaagt tatgaagtct tacacctttt cttatgtata aagctcctac
     9121 accctttggt ccatatattt tatgtgaaga catactcatc aaatctattc ctaattcttt
     9181 aacatctact ggaatattac ctgtagcttg tactgcatca gtgtggaata atatcttatg
     9241 ttttttagct atttcggata tttctttaat aggctgtata gttcctattt cattatttgc
     9301 aaacattatg cttataagta tagtagtatc ttttattgaa tcttctagtt gtcttaagtc
     9361 tactcttcct tcgctatcaa catctaaata agttatttca aatccatgat gttttgctaa
     9421 gtattcacat gtatgaagta ttgcatggtg ctctattttt gaagttatta tatgattacc
     9481 tttgttttta ttagcataag ctacaccctc tagtgtccag ttatcacttt ctgaaccacc
     9541 tgctgtaaaa tatatttcac taggttcagc atttataagg gctgctactt gttctcttgc
     9601 tttatccaaa gcatcttttg cttctcttcc gaatgtgtga aaactagatg cattaccaaa
     9661 ataatctgtt aggtatggca tcattgcatc caatacttct tttttaatag gtgttgttgc
     9721 agaataatcc atatacaatc ttcttttttc cattacattc atctccaaat ctatttttta
     9781 atatttgtta tatcattttc taatttattt ttgttatagt catttatcat atctttaaga
     9841 gtaattgaat ctataacatc ttctataccc tttttcatct tttcccaaac tatttttgtt
     9901 acacacatgt ttgagttttc acatacatct tcatctagta cacagtcaga taatgcaact
     9961 ggtccttcta atactacaag tatatctcct actgttatgt cttcagcatt ctttgaaagc
    10021 aaataacctc cttgagcacc tctcacactt tttactagac ccgatttttt taaagatgaa
    10081 aatatttgtt ctaagtactg atctgatatt ttttgcttct tagcaataaa ttttaatgat
    10141 actggtccat tgtcttgatt taatgctaat tcaaacattg cttttagtcc atatctacct
    10201 ttagtagaca acttcatata ttatcacctc tatatagaaa tctaattcat actgttttag
    10261 taggaattac tttcaagata attttattat accctactaa ttttgtcaag attaatttta
    10321 attttttatt ttgaataaat tccccatttt ccaatgtttt caaatcctag ttttttatat
    10381 atctttccag cctcttcatt atcataaaac aaacaaggaa ttttgttttc tctcagtaac
    10441 tctgaacata gtttaattag gcattttgta gcaagtcctt tagctctata tttaggatgt
    10501 gttcctactc caattatcat tgcatgagtc ctattttctg aagtactctt tgccattgac
    10561 accacctgtt tgttaatctc tatacaatat cctctacctg tccttagtcc attttttaaa
    10621 ttctctaaag ttgtactgtg aaattcatct attaagtcat aaagtttcac tgtctttttt
    10681 agatttccaa gcctaattct cttaaccttt gcattacaat tattatctat caaaaacttt
    10741 ttagtttgaa gtttgcaaaa atcaactacc tttaaattat taagtcctaa tctctttgct
    10801 aaagtatcta cagcacatat tttcccagat atttcttcgt aattagtatt acgaagtaga
    10861 ttataaaaac cttcaacatc atactctcca tcagaataaa acatcataaa ttcaaaatat
    10921 ttaagtaata ttgcctttat ttcaccatgt tcacccacgt ctgcccatat atttaaaaaa
    10981 ttatttccat atccatatct ttcaatatcc cctattataa acaaattgta ctcaggttct
    11041 ttttttaagt atttcataac tctactgtga taagttgagt taagttttat aatcataata
    11101 tctacccccg atacataaaa ttcagtctaa aaatcactat ttagatattc taaatatttt
    11161 tttatatttt tctcagtttt attttcttgt ggtatgtaat aaatactatc ttttatgcta
    11221 tctggtaaat actgttgctt tacataatga tttttaaaat tatgaggata tttgtatcca
    11281 caacccctac ctatttcttt agaaccagaa tagtgagcat cctttatact atttggtata
    11341 tccttaacaa actctttatc cagttcatcc atagctttca ttattgctat acatgcagaa
    11401 ttagactttg gagatgtagc caaaagaata gtagcttctg ctaatggtat tttcgcttct
    11461 ggaaatccaa gttgcatagc tgagtctaca catgccttta caattacaat agcatttgga
    11521 taagctaaac ctatatcttc actagctatt actaaaagtc tcctacatat acttatcaaa
    11581 tccccacctt ttataagtct tgctagatag tgtatagatg cctgtgggtc acttcctctt
    11641 atagattttt gaaatgcact taaaatatca taatgactat caccattttt gtcatagttt
    11701 ataattttat taaatgtaga tgcccttatt acatctgaat ctatataagt tatattatcc
    11761 ttatttggtt tagttgaata gactaccact tccaaaatat ttaaggctct tctcatatct
    11821 ccatcagaaa gaattgctat atcttcaata gcattattat tgcactcaat atccatatag
    11881 ctatcttcat ttaatacttc aacagctctt ttcagtcctt tttctatatc aagtttttct
    11941 aaaggtttaa attcaaatac agtagaccta cttaaaagag ctttatatac ataatgatat
    12001 ggattttctg tagtgctagc aatcaaagtt atactcccat tttccataaa ttctaatatt
    12061 gattgttgct gttttttatt aaaactttgt atttcatcta tatataatag gactccattt
    12121 acattattta tacttccaag ctctgatatc acctttttta tatcttctaa agatgaatta
    12181 gtagcattta ttttatagaa gcttttattg cttctttctg ctattatttc cgctactgta
    12241 gttttaccaa caccaggagg accaaaaaat atcatatttg gtaaaaaatt agattgtaat
    12301 atcttagaca atatttttcc attacctatt atatgacttt gacctattac atcattcatt
    12361 gtttttggtc ttattttatc tgctaaaggc attttctcac tcctttttaa tatacttatc
    12421 attaccttaa taattcgtct aaaaaaggta tctatttgaa atagatacct tttctaatct
    12481 aaacctataa aatatagatt attgattgtt ttttaatttt tttaattcat ctgtaagttt
    12541 gtcattaagt attcttatat gagtaccttt catacctaat gatcttgatt ctatcacacc
    12601 tgcactctca aattttctaa gtgcatttac tattacagac ctagttatac caactctatc
    12661 agctatctta cttgctacaa gtagaccttc ttttccatcc aattcagcaa aaatatgttc
    12721 aactgcttca agctcggagt aggacagagt gcctattgcc atttgaacta cagctttctt
    12781 tctcatttct tcttctaatt cttcacctat tgctcttaat atttcaagac caacaacagt
    12841 tgcactgtat tcagctatta ctaaatcatc atcattgaat gaatttgaat atcttgaaag
    12901 taccaatgtt cctaatcttt gaccgcttcc taatattgga actactgtag tatatttttg
    12961 taatcttcca tgttcttcag gaaagatttc taatatcttc tcaccattta aattttctaa
    13021 tgtttcatca atttttaaca cattttgagt atattcatct gaaaatttct tttgcttagt
    13081 atactcatct tctataactg aactgtcttg aacatcattt aaatgaagac ctagtacttt
    13141 accttttgca cttactacat aaacattaga acttaaaacg tcgcccaatg ctccggccag
    13201 taaatcaaaa gagacactgc ttccaccact tgtttgtaat gttttattta ttttccttgt
    13261 tttttgtaac acttcacttg ccatttaatc atctcctcat gaaatttagg ccttttaata
    13321 accttttata ttttctaata ttattttcac atttaatgaa aatttatttt tctattagtt
    13381 gtttacactt gaaagattat catagattgg aacctttttc aagagaatta tgtgattttt
    13441 ttcaattttc actcttttcc gtcattatgc tcttttgtct ctttattaca ttctttatta
    13501 gaacacactg tttttgtttc agatttagtc actttttcaa ccatatatga accacacttc
    13561 tcacaaaact ctcctgttgg cttattccaa gctacaaagt cacagtttgg ataattagaa
    13621 cacccataaa aagctttacc tcttttagat tttctaagta taatatctcc ttcttcacat
    13681 tttggacatt taacaccaat tttattcata attggtctag ttgttttaca atcgggatag
    13741 tttttacaag ccataaactt tccaaatcta ccatatttga taaccatatt agacccacag
    13801 ttctcgcaaa tctcatcagt ttcttcatcc atatttactt tttctatatt ttcaagagct
    13861 tcttcaatag cctctttaag tggtgcatat gattctgata caatcttttt ccaatctgta
    13921 ttgccttctt ctataacatc aagtttactt tccatatcag cagtaaaatc tacatctata
    13981 aatttatgga aattattttc caaaatatca gtaactatta ttccaagttc tgttggacat
    14041 agacttgttc cttgcttttc aacatactct ctattcagta tagtagctat tgttggagca
    14101 tacgtacttg gtctacctat acctagttct tctagtgttt ttactaaact agcttctgta
    14161 tatcttgctg gtggttgtgt aaaatgttgg tctggcaata tattttctat atttaatata
    14221 tctccttttt ctatacttgg taaaattttg tcttctcttt ctgaaaaatt atataccttg
    14281 gtataaccat caaatttcat ttttgaacca gttgccttaa atacaatatc gcctatttta
    14341 cattctacat ttagtatatc aaacactgaa tcttccattt gactagctac aaatcttctc
    14401 caaattaaat tgtataactt gtactggtct ttgctcaatg aggcttttat actctctgga
    14461 gttctatcaa ctgaggttgg tcttattgcc tcatgggcat cttgaacctt tttagtatcc
    14521 tttttttctt ttttatcacc agtgttttta tagtagtttt ctcctaaatt gtttacgata
    14581 taacccttag ctttttcttt tgcttcatca gatattcttt tagagtcagt tcttatatat
    14641 gatataagac caacagtacc ttcaccttct atgtcgatac cttcatataa ttcttgtgct
    14701 atcatcattg tttttttagt agtaaaaaat aacttatttg ctccttcttg ttgaagcata
    14761 cttgttgtaa atggttttgg agctgattta cgtcttgatt tagattctat cttctcaacc
    14821 tttaaatctt taccttccat ttcttttagt atttcattta cagcttcttc attttctaat
    14881 tcaatctttt catcattttt accataaaat ttcaaagtta tatctttgtc ttttactgtc
    14941 ttagcctcaa catctattgt ccagtattct tttggaataa atgcctttat ttctttttct
    15001 ctatcacaaa ttaactttgt tgtaacagat tgcactctac ctgcactaag accttttcta
    15061 accttttgcc ataatatagg acttatttga taacctaaaa gtctgtctag cactcttctt
    15121 gcctgttgtg catcaaccaa atttatatca atatttctag gatgtttaat tgctttttta
    15181 attgcatcct tagttatctc attaaactct attctacaat cctcagattc atctaaattt
    15241 agtatatgag ctaaatgcca tgatatagct tcaccttctc tatcagggtc agttgctaga
    15301 tatacttgtt tagatttttt agcttctttt tttaattctt ttattacatc gccttttcct
    15361 cttatattta tatattgagg ttcaaaatta ttttctatat ctacacctaa tttactttta
    15421 ggtaaatctc tgacatgacc aactgatgct ttaactgtgt aatgactttt ccctaaaaat
    15481 ttttctatag ttttagcttt tgcaggcgat tctacgatga ctaatgtttt ggccataaaa
    15541 ccatacaccc ccactaccta atcttaattt atacatttaa actgtacgtt ttattattca
    15601 tttctactac taactcattt agtactaatt cattgattat tgaatttaca tactttattt
    15661 ctataccagt ataatcacaa atactatcta tatgcaagtt tccttctctt tttatagctt
    15721 caattatttg cttagacttg gcatttagac ttatattatc atatttttga tttatttttt
    15781 tatcaataat attaaatatg ttatattcat ttaaaatatc atctatattt tcaattaatt
    15841 ttgcaccttc ttttataatt ttatgacaac ctctactcat ttgagaattt atattaccag
    15901 gtattgcaaa aacatttttt ccatgttcaa gagcaaaatc tactgtgatt agtgcaccac
    15961 tttttattgc tgcttctaca acaactactc catcacttaa accactgata attctatttc
    16021 tattagaaaa atggtacggg gctactgtcg agttaatatt atactctgat aataataatc
    16081 ccccattttc taatatttta tttgataaat gtaaattttg ttttggaaga ggattgtcta
    16141 ctcctgaacc aagtactgct atagttttag attttccact catacaacct atgtgcgagt
    16201 atgcatctat tcctatagct aatccactta ctatattaac tccatattgg gatagttgac
    16261 aacttattgt ttttgcacaa ttaattccat aggctgtagg ttttcttgaa cctactatag
    16321 ctatattaaa attattatta actaaaccta tgtcaccttt gtagaataat aactttggag
    16381 cgttgtatat attctttaaa ttttcaggat attctttatc atatttacaa atatatttaa
    16441 tcgctttttt gtaaagtagt tcttttatat tttctaaata agcgtaacct ctatatttta
    16501 ctatattttt tctaatattc aagcttatat tttttaaatt atatatttct ttttcagaaa
    16561 aatcaaatat atcttcaata tttactattt cattttctat tatttcaatt gtttttgttg
    16621 ttattccacc aattgatttt aaccataaat aagtatctct tttttccata aaatccacct
    16681 caaaaaacta tttaaaataa ctataataag cctttctaaa tgaaaaagct tctataatac
    16741 actgtgaatt tatcaagtct ctttcttcta aatctgctat tgttcttgcc atttttaaaa
    16801 gttttgtata acttctactg cttaggttgt atttattaaa tatcatctca gccgtttttg
    16861 aagcttcttt ttccagttta caatatttaa ataaattgta agctttaatt tcactattat
    16921 tttttatatt atctttctta aatctattta tctgtatttt tctagcattt tctactcttc
    16981 tctttatttt ttgagagctt tcttcttgtt ttaaagaatt aaaatctgaa tattttatag
    17041 aattaacttc tacaaaaata tcaaacctat ctaacaatgg acctgatatt ttatttatgt
    17101 atctatcaat ttcgttactt ctacatctac actctgtctc tgacatataa taaccacatg
    17161 gacatggatt catagctgca actaacagta ctctacaagg atattttgca ctgtacttta
    17221 ctctggatat atttacatat ccatcttcta taggttgtct taaagtctca agtatttttc
    17281 tatcaaattc ggctatctca tctaaaaata agatacctct atgtgctaaa gcaatttctc
    17341 caggtcttgc atccattcct cctccaatta aagattgctt tgtagttgta tgatgaggtg
    17401 ctctaaaagg tcttttatca attattcctt tactttcatt tatcataccc aatatactat
    17461 aaaccttact tacttctatc atttcttcta tacttatatc tggtaaaatt gtccttactc
    17521 tttttgcaat catagttttt cctgagcctg gaggtccaat cataaacata ttatgatttc
    17581 cagctgctgc aatttctgca ctcctcttta caaaataatt tccttttaca tctttaaagt
    17641 cttcatcata tttactttct tcattattaa tcttagtata tttgcaatct atataactat
    17701 tatctttttc taattctccg ttctcttttc ttgacttatc atccaaaaaa gacattatac
    17761 ttactttatc gacttttatc tcttcgttta aaaaatcgac acattctttt aaacttttaa
    17821 taggaattat atcaatttca tctactaata gactttcttt tatgttctca attggtataa
    17881 aaatacgttt tatgttctga gtttttgcac ccataattat gggcaatatc cctcttactt
    17941 ttcttatttt tccatctaat gataattctc ctataaacat actttctctt atgtaatttt
    18001 catctttttt tattaactct cttaaaaggc ctatacttat agataaatct aaaaaagctc
    18061 cttctttttt tatatctgct ggtgataaat ttacaactat tctcgtgctt ggaaatttat
    18121 atccactatt aagaattgct gactttactc tttctcttgc ttctttaatt tctttgcctg
    18181 gaagaccaac aatattaaaa ctaggtattc cattgcttac atctacttca accttaacta
    18241 aaaagctgtc gattcctact aagttgcttg aattaataat acttaacata tttaatcctt
    18301 tccaaaaaca ttatcttttt taaaaagcat tcattatgtg atttattctt ttttctaaca
    18361 aatagacctc tatgatgtca aatcttatgg gagtattatt taagttattt tttataatat
    18421 agtattttgc gacaccaatt atcttttttc tttttttaaa atctacagat tcacaagggt
    18481 agccatactt tatgttagac cttgatttca cctccacaaa tacaacttca tctcctatat
    18541 ttgctattat atctacctcc cctatcctta atctataatt tttttctagt atctttgcac
    18601 cttttgatag cagataatta actgctattt cttctccaaa atcacctttt tctttattat
    18661 tcattatcta ctccaatata ttaaattttt acttttaaat tatcgtcagt atacaattta
    18721 actattcaat tttttgaaat tgattttatt tccattgata ttaataaata cttctattga
    18781 ctttattctt ttatgatatt atgctagttt ttataatgaa agtttatttt tatggaggaa
    18841 tgtctttaat gaacagaaaa aaaacattta tttttatacg atatttatct ctatttatat
    18901 tacttataag ttatttaaac catactgaaa atacattgta tccagctata acaatgatta
    18961 tattatttct aataataaat aatcaagtta gattt
//
LOCUS       DAMJVX010000064        18942 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMJVX010000064 DAMJVX010000000
VERSION     DAMJVX010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.067"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(87..2057)
                     /gene="ftsH"
                     /locus_tag="PC407_001979"
     CDS             complement(87..2057)
                     /gene="ftsH"
                     /locus_tag="PC407_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2497425.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2091..3479)
                     /gene="tilS"
                     /locus_tag="PC407_001980"
     CDS             complement(2091..3479)
                     /gene="tilS"
                     /locus_tag="PC407_001980"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDF2497426.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(3662..4090)
                     /locus_tag="PC407_001981"
     CDS             complement(3662..4090)
                     /locus_tag="PC407_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDF2497427.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEENIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4107..4913)
                     /locus_tag="PC407_001982"
     CDS             complement(4107..4913)
                     /locus_tag="PC407_001982"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDF2497428.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5115..5600)
                     /locus_tag="PC407_001983"
     CDS             complement(5115..5600)
                     /locus_tag="PC407_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HDF2497429.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(5651..6310)
                     /gene="spoIIR"
                     /locus_tag="PC407_001984"
     CDS             complement(5651..6310)
                     /gene="spoIIR"
                     /locus_tag="PC407_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HDF2497430.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6455..7135)
                     /locus_tag="PC407_001985"
     CDS             complement(6455..7135)
                     /locus_tag="PC407_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2497431.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(7119..8009)
                     /locus_tag="PC407_001986"
     CDS             complement(7119..8009)
                     /locus_tag="PC407_001986"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDF2497432.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8127..9677)
                     /locus_tag="PC407_001987"
     CDS             complement(8127..9677)
                     /locus_tag="PC407_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HDF2497433.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9771..10037)
                     /locus_tag="PC407_001988"
     CDS             complement(9771..10037)
                     /locus_tag="PC407_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDF2497434.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10185..11045)
                     /gene="yabG"
                     /locus_tag="PC407_001989"
     CDS             complement(10185..11045)
                     /gene="yabG"
                     /locus_tag="PC407_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HDF2497435.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11129..12067)
                     /locus_tag="PC407_001990"
     CDS             complement(11129..12067)
                     /locus_tag="PC407_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HDF2497436.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12177..12338)
                     /locus_tag="PC407_001991"
     CDS             complement(12177..12338)
                     /locus_tag="PC407_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497437.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12348..12845)
                     /locus_tag="PC407_001992"
     CDS             complement(12348..12845)
                     /locus_tag="PC407_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2497438.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(12960..13670)
                     /locus_tag="PC407_001993"
     CDS             complement(12960..13670)
                     /locus_tag="PC407_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HDF2497439.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(13846..14376)
                     /locus_tag="PC407_001994"
     CDS             complement(13846..14376)
                     /locus_tag="PC407_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497440.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEIVKNVDCN
                     IVQYSNKDERRVKAKANKMAMRLIHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(14393..14623)
                     /locus_tag="PC407_001995"
     CDS             complement(14393..14623)
                     /locus_tag="PC407_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497441.1"
                     /translation="MSKLVSKVKKDLEIKWLGVDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(14638..16032)
                     /locus_tag="PC407_001996"
     CDS             complement(14638..16032)
                     /locus_tag="PC407_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2497442.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTI
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(16158..17411)
                     /locus_tag="PC407_001997"
     CDS             complement(16158..17411)
                     /locus_tag="PC407_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2497443.1"
                     /translation="MENKSNFKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALSLS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGVLGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(17462..18616)
                     /locus_tag="PC407_001998"
     CDS             complement(17462..18616)
                     /locus_tag="PC407_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2497444.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRKLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIKKLK"
BASE COUNT         5850 a         3610 c         1865 g         7617 t
ORIGIN      
        1 tttgcttatt aatttaatat tatactttca gttttagtat ttaatccaca tcagataata
       61 atcttaaaat gaccaaggtc ttaaaactat ttatctttat cagatttatc ttctaaattt
      121 ttatttatat caataacatt gtttctttct tcttcatctt tttctaattt tatagttaat
      181 tgttcttcaa cttctttaga agaattttcc tctgtaactg cattttcaat ttcattcaga
      241 ggtaactctt tattaaatgc ttttacaaat tgttctgcat ctaaagtttc atatataagt
      301 aaagcttgtg ctacatactc taatctatcc atattttctt gtaatatact tcttgtctta
      361 tcatatgcac catcaactat acgctttgtt tcttggtcta tctcaaaagc aacttcttca
      421 gagtaatttc tcttacttga aaaactatta cctaggaata cttcatcatc actgtcaaat
      481 gacattggac caagtttaga actcattcca tactttgtta ccatacttct tgcagtagct
      541 gtaactcttt ctaagtcatt tgaagcacct gtagatacat ccttaagtac tagctcttct
      601 gctactctac caccaagtaa aactacaata ttttctttca tttcattttt tgtagcatag
      661 aacttatctt caacaggaag ttgcattgta aaccctccag ctcttcctct tggaactatt
      721 gtaacttgat gaacaggact tacttcttca agtacatggg cacaaactgc atgccctcct
      781 tcatggtatg ctgtaagtct tctttctttc tcacttatta ctttcgattt tttagccaca
      841 cctgctataa cctttgttat agcttcttct atagtttcca tcttaatttt cttttctctt
      901 tttctagctg ttaatattgc agcctcattc attaagttct ctatatcagc aggagtaaat
      961 cctggtgttc ttctagccaa gacatccatt tttacatcat cacttaatgg tttatttctt
     1021 gagtgaactt taaatatagc ctctctacct tttacatctg gtgtaccaac aacaacttgt
     1081 ctgtcaaatc ttcctggtct aagtaaagct gggtcaagta tatctggtct atttgtagct
     1141 gccatgatta ttataccttg atttacccca aatccatcca tttcaactag aagttgatta
     1201 agagtttgct ctctttcatc atgacctcca ccaagacctg cacctctttt tctaccaact
     1261 gcatcaattt catctatgaa tatgatagct ggagcacttt tcttagcctg ttcaaataaa
     1321 tctcttactc ttgaagcacc aaccccaaca aacatctcaa cgaaatcaga accacttata
     1381 ctgaaaaagg gaactcctgc ttctcctgca actgctcttg atagatatgt tttaccagtt
     1441 ccaggaggtc ctaccataag cattcctttt ggtattcttg cacccaattc aatatatttc
     1501 ttaggattct ttaagaaatc tactacctcc tgcaaatctt ctttttcctc atctagtcca
     1561 gctacatctt taaatgtaac tctagttttt tcatcatctt tatgaacctt agcttttgat
     1621 ttgccaaaac tcataacctt tccgcctcca ccttgagact gattcataaa cacgaaccaa
     1681 ataatcacca tgaagaatat taacagtaat gtaggcaaca tttcgacaaa ccaaggtgta
     1741 gatggcttag cttcaccacc aaaagttagt ttcccagcct tagcttggtc taacacctca
     1801 tctgcaagtt tatttcccat cacttctgtt ggaacatatg acttaaactt tgtatttgta
     1861 tcttttatag taccttctac agaagtttga tttacaaagt ataatcttga tatattctca
     1921 tctgttaatt ctctatatac ttcagagaat tttaaatctt ttattttttc tgtaggctta
     1981 cctgagaatt gaactatacc tactataatt ataaaaacaa gtagataaaa acctgcccct
     2041 tttagcaact tattcaatat agaagccctc ctttcataca ttgctttgcc ctataatttg
     2101 ttaaaattaa tttttaatac ttcttttgtg ttttcatcaa ttttataatt ttcactgatt
     2161 ttataatctc caacacatat tattccttca ctatctgtga ttataggaat tttacatctt
     2221 tcttctcttg ggatttttaa atctatgaat aaatctttca cttttttact tcccatagca
     2281 agctttatct tatcaccttg ccttctgctt cttattacta tacccccttt taccttatta
     2341 aaatcaaacc ctttagatga attgtctaat ttcatactct tatacctatc tatgctcatt
     2401 acttgggtct caataatttg ttttaattct tttattttaa taaacccatt actaggtaca
     2461 ttgtaataaa attcaatttc ctcattaaca atttcctcat tcgttaatat tatactgtct
     2521 ttttttctat aaacaaatat atttcttgga agagttaaca ttttatttac ttttgaatta
     2581 tcttctaaag acattatatc ttctatatgt ctttggtcaa caaaattagt atctcctagt
     2641 atatgtttta tggaatttct aagaactcta actttgatag catcatgtaa gcaaacaaaa
     2701 tcgtctaaat ttatctctac aaagcctttt tcttttatat ttgaaacttc tctaaattta
     2761 gcttctgcct ctttctcaat ataatcgtta tcacttttta aactattgct catcctcact
     2821 atagattcta ttacatttga attaaaatta tccttcatat atggtaaaag gtctaacctt
     2881 atcttatttc tagtgtatat attttctaaa tttgttttat ctatcctcgg atttaaatta
     2941 taagcttcac aatactcctc tatctcattt ctttccacgt ctaatatggg tctaattatg
     3001 caattatctc taatataatc aattcccttt aagcctttta agcctgttcc tctcatgata
     3061 cgcatcataa cagtttcggc ttggtcattg agattatgac ctattgctat tttattggct
     3121 ttaatcttat ccttaatttc ataaaacatt tcatatctta gcttccttgc tccttcttct
     3181 aatgataatc cctcattttc acaatatttt ggtacattaa tagactttac aaagaatata
     3241 attcccatat cctcacaaag tttagataca tataatgcat ctttttgagc ttctatccct
     3301 cttatttgat ggtttaaatg tgctgcatat atttctatat taaaatcttt ttttaatcta
     3361 tttagtacat gcaataaaca aactgaatca ggtcctccag aaagacctaa aactatttta
     3421 tcgccttttt gtattaaatt atgtttattt atagtactta gtaccttatc aaatatcata
     3481 tttatccctc ttacacttag ttttattcac ttttaagtat actttctata aaaaaacgct
     3541 aaacccttgt atcaatttta agaaattaaa gtttaatata aatataataa taccaaataa
     3601 attaaataaa aggtagtttt ttcactacct ttataaaatt tctggaaaat agccatagtt
     3661 attatttagt acctatgtat atatctattt tattcctacc taaaaataaa tcttgtacct
     3721 ttatattata gtctattttc aattttatgt gttccccttc ttgtatattt atatctaatt
     3781 ctcttgtatc aatatcaatt aataaagtac cttgtggggt cacatacttt gtagtactac
     3841 tttctccaca tttaaacagc attgttgagt taatagcacc aaactttttg atactaactt
     3901 gatttttaag tatctttata gtattagtta tttctatatt ttcttctttt tccttgtaaa
     3961 taacatatat tccatcattt ttataaaaaa ttttaccaaa ggcagtcatc tctatggtgt
     4021 ccatatttcc tttttcatca tattgtctag tatttatact tatttttaca ctcaacgatt
     4081 tttcctccat agattcttca tataaactag tatttttgta tttctacttt ttgaatctta
     4141 tcaatttttt tctttaagaa ctctcttgct atatctgaaa atttttcagg tatatctgat
     4201 acataatatt gataagtccc atgaatttct gtattgttta taatattttg cacctctaaa
     4261 atctttttta aatcctttgc agtctcttta gctggattaa caagttttat atgctctcca
     4321 acttcttcac ctattgttct ctttaaaata ggataatgag tacatcctaa cactaaagaa
     4381 tctatatttt tttctttcaa ttcttgtaaa tatatctttg ctgtcaattt agccacttct
     4441 gtatttgccc atccttcttc aacaataggt acaaataaag gacatgcttt attaacaatt
     4501 tctatactct catctatttt agatatttcc aagttgtatg cttttgaact tatagttcct
     4561 tcagttccta ttattcctac aattttgttt tttgtagaac ttacagctgt tctagctcct
     4621 gcttctataa ctccaattat cggtatatca tatttttctt gagcttcttt taagcttctt
     4681 gcagtagcag tgttacatgc tataactata gcttttacac cttttgtttt taaaaagttt
     4741 atagcttgaa aagtatattt aattatagtt tctttggatc ttgaaccata tggtattctg
     4801 gcagtatctc caaagtatat tatatcctca tttggtaata tcttcattat ctcttttaga
     4861 actgttaatc cacctaatcc cgaatcaaaa actcctattg gtttattgtt catctttata
     4921 caacacctac tttacatttt tgtgtttaac aacaacttat ttatcgctaa aatttatatt
     4981 tatattcata attaatatat tattacctaa atgattatta tagtttattt tacacaaaaa
     5041 ataaaggagc tatcttaaat tagaaaataa tttctacaaa aagacaactc ctttcactac
     5101 ttaaaaattt aaatttattt tgcaaacaca tgatttccta ttctcatata aggattcaat
     5161 ctatttatcc atttgcaagt ggctatttct ggattccaaa aatatactgc tttattagtt
     5221 gggtcagttc cataaagagc ttcctgagct gctttacgtg ctgagtctgt tggtgccatc
     5281 tttattgaac catcaagcac aactgagaaa gcattttttt gatatatgac ttcttctatt
     5341 gtgtctggaa atctagggtc caatactcta tttattacta cagctgctac agcaacctgt
     5401 ccctcatagc tttctcctct agcttctcct gcaaccaact tagataataa catcaagtca
     5461 ttatcagata aatttattac ttctttcttt gctatatttt tttcttgttt gtttactttc
     5521 ttaatatttt ctgttggttc atatgcaaat acctctttaa taggcataaa tataaaagtc
     5581 aaagctaata gtgtagccat cagtttactc cacctgtcca tgattaaaac cactccttac
     5641 attcttattt ttaaaatttt ttctctaaat ccttgactac ttcaagtact ttaaacttaa
     5701 tttttactct acttgttttt tttggtgtat cttgttttat taacttatat tctttatcag
     5761 ttaaaacttc cttcaactta tcatctgtag atttatctat cactccatta gattcatcaa
     5821 caaaacatag tggagggaac ataacacacc accagttttt tccttcacct ttcccaatga
     5881 taatctttaa tgctttatat tcaccagctg gaagaactat attagaatat tgtttagtag
     5941 ggaaattgct atattgtata cctaccttta cactgtaatt atatccattt ttaagaataa
     6001 tatttttact tatttcttcc agactactat attccttttt tataatagct tcactttctt
     6061 ttatactttt tgaattttga agctttggct gtaaatattt tattacttca tctctaactt
     6121 ttagtttaag ttcttgatct tcatctgtat tgctgtttgc tataacatga aatcttatta
     6181 atttatcttt ataatctttt gatatattgt ctactttttt tacctcacca tttattacta
     6241 ttgtcattat tgatatgact gatataagac ttaatattaa tagtcccaat ctaatcttaa
     6301 tttttctcat tgaaaattcc ccctcaagat aaatttatat catcaattat gtccaattaa
     6361 atttaattta ttcataatat tttttaatac taataaaaaa agctatctta aaatgattac
     6421 aatcatttta aaatagcttt ttaaacctta tattttataa gtctttacta atcatagcct
     6481 tatctatcat aaactgctct gctttttcta tatgctctgt agcatacttc ttggatagct
     6541 cactatcacc tttaattata gcatcaagta agtatttatg ctcttcaaca atattatgtg
     6601 atgtatcata agcagacata taggtaactc taaatctata tacttgttcc cataatgagt
     6661 ttattagttg gattaatttt ttattattag ttgctttaaa tatacattca tgaaattcta
     6721 catcaagttt taataacgca tctacatcgg attccaaagt gcttttttta aattcttctg
     6781 atatatcttt aagcttttta atatcttcat cactaattct ttcagcggca agatatgctg
     6841 ctaagccttc taggcttgct cttacttcta atacatccat aatatccttt aatgacatat
     6901 ttgccacata cgcacctttt ctaggcaaca taacgactaa accttctaat tcaagctttc
     6961 ttattgcctc tctcactgga gtcctactta ctcctaattg ttctgctagt tgcacttcca
     7021 ttagcctttg accaggcttt aattttcctt ctaagattgc ttctcttaaa ttctcaaaga
     7081 ctacatctct taatggcttg taattatcca agttcagttt agttaaattc tccacaaatt
     7141 tcaacccctt tttgactgct attaacaaca tacacctgtt tatatttttt taataattct
     7201 ttttttccaa ttagagcatc ttctttattt ttaaatagtc caaaaactgt aggaccactt
     7261 ccactcatca tagaacctaa agcattattt ttcatcatta cttgttttat atcacttatt
     7321 tctttgtgtt tacctatagt aacattttca agaatattta ccatgctttc tgatacagct
     7381 tttatatctt cagattttaa acactctatc aaatatttat tatttggtct ttttttaata
     7441 ttattcaaat ctaatccttg ataaacttct ttagtagaaa cgaataagtc tggtttgcat
     7501 atcaatatat ttgtatcaca tggtaaacct ttgatgtttg ttagcttttc tccaatacct
     7561 tgagctaagg caggtcttcc tgatatacaa aaaggaacat ctgcgcccaa attaagtcct
     7621 atttctttta attcatcttc agataatcta agttcccata ggtgatttaa acctactaat
     7681 actgcggctg cattagaact tcctccagcc atccctgctg ctacaggaat atttttttct
     7741 atgaatattt ctacaccttt ttttatataa aatttatttt taagtatttt ggcagcttta
     7801 taaacaatat tatcttcatc taaaggaata tctaaactag tgctttttac ctttatttca
     7861 tcttcgtcta attcttttat ctttactata tcgtataaat ctatcgtttg cattatcatt
     7921 tcaacaaaat gataaccgtc ttgccttttt cccaagacat caatggataa atttattttt
     7981 gctctactct ttagtcttat aaaatccatt ctatcctcct tgtttaaaga ataatgcatc
     8041 actaacaata atattatact acaaaaaata aaataatagg gtattataat accctattat
     8101 acttaattac tttaaatttt ttaatattaa tctactaata cgactttttt ttctaaaata
     8161 agacacttac caggcttaat tggttcatca agttttatat cattaagttc tgcaatttca
     8221 ttttctgttg tattgtattt ttttgctata ttccagaaag tatctccttc tttgcatata
     8281 tatacgatta tgctaggagc tttagataaa tcatatacac cttggtcttc tcctttgata
     8341 ataaaatttt ctgctttctt atctaatgct tcagtaaatc tcttaatttt gataatcaag
     8401 tctatctggt ccctattcaa atcaacttcg actttatcaa tacatgctgt attaaacaca
     8461 gatgctgtat cagttagatt atccatttct atatcatgtt caaatggaat ttcctcacta
     8521 attttataaa ccaattttaa accttcaact gggacaaata aaatttcaac ttttatgatt
     8581 ccttgtataa tgcttttatt tccttcaata taactatttt ctatagacat ggttggacaa
     8641 acgcttacaa tatcttttat ttgtatatca tcattatcat ttcttatgcc ttctctaacc
     8701 ataaatgttt cttcactacg tcttaaagtc ttatttaatt gaataggttt gtggtcaaat
     8761 tttattatct tttgaggcga atatgcatct tgtaatactt ctcttgtaac ttcatctgta
     8821 acttttacct tacaacaagc catacaatct atttctaaaa gacctgtgtt gctttcacta
     8881 ttttgcttga aaatatgatt aaaatctgac atagacaaca atacttcttc agtcatacca
     8941 tcactaactc cagggacttc tacaaattga gtaaactcaa tacctactct atccaactct
     9001 acaagttcac cctcatatgt acaagctaac ggatttattt ctaatactcc accaataata
     9061 actttattgt cagtcactct actttctttt actttaacac atggatttaa acttattata
     9121 gattgaattt cctctgtgtt tattgttata gtatctctta tagaactttc tgctttttct
     9181 attccaacta tatcttgaaa acaaatttcc tttctgtgct tttgtatgcc ttccacttgc
     9241 gccacatctt ttacaatgtc taacctttgc ttttcaaaca aacttcccct tatattcata
     9301 agagctccta ctttaatctt tctctcattc attatagtac aatccatatg ctctacttct
     9361 gaaaataaca tgtattccat atcttgaaca acattgtctt tttctattac ttcgtttata
     9421 tctacttttc cgtctacatt agaaatagta tttttatcat ctgcaatata gattacatta
     9481 taattaaaac tacctctgca aagtatcttc ccatctgcca tttcaatttt tttaagtgat
     9541 atgtatcctt ctgtttttac aatttcatat acatctaatt ttttgtctgg tacaactgcc
     9601 tccgcttcaa tgaatgtttg aaattttcca aagtctattc tattgtcaac tttaattaca
     9661 tctttaatta attccatagt ctacctcctt aagtattata aaaatatcac actatattta
     9721 tatttaagga gcaaaaaaaa aattacacct atttttaaat aggtataatt ttaacttatt
     9781 tgtaatttat cttgatctct aaaaacttgc aactttacat tagatgttaa taaatctgag
     9841 taactataag atactctagg atatccatta ctttcatcat ctaactttat aacaaacacg
     9901 cttggataaa ctttttctag tataccctct tttgtaataa tctgctttct tcctttatta
     9961 gctttcaaca atattttctt tcctatatgt ctctctaagc ttactcttat cttatctaga
    10021 gtttgaacag tagccacaat atcaccttct ttaacgttta tatgtttaca taatatcaca
    10081 tataaacatt tttgtcaaat gtttaaacta attattttat atattttttg caaaaatgtc
    10141 aatactattt ttgaagatat acagtataat tatgttaatt tttgctaatg taatattgtt
    10201 tttggcattg cccatctaaa ttttcctctt gtttcagaac caccaattcc cttaacacct
    10261 gtaatagtct gctctataac ctcatcaata ggaactacta catcaattct tgaacaagca
    10321 attttctcca ctataaaaac agggtctaat gcatgtatca ttattcttcc aggcgaactt
    10381 gcataatttg ctcctgcact tattattttt tcataatttg attggcatgc tcctgcgaat
    10441 atgacaagat tatccaaatt tggttgccat ttacgtgctt cttttactgt tttaacaaaa
    10501 tttgaagagt ttctatagtt actcatatct tctatatctc ctctttttat tgtcattgca
    10561 tcatgacctg ttattactag tatatccgga ttatattttt ctagtagtgc tcttacttct
    10621 ttatactgat tttgctctga aacagctaca cctactgcag gtattccaag ttgagcatat
    10681 acatccaagc aaatttttaa atattcttta tctccatcta tctgtagtac ttttccaggc
    10741 attccatatg tgttaggatt taattgaagt tttggaactg ctctagtcat tcttttttgt
    10801 ctttctttag cttttcttac tgacttatat aataaatttt ctacattttt atctattaat
    10861 atatctttta tatcaggtgc ttttactaat tctaggtcat caatataagc atctgctatt
    10921 actctaaatg caattccctt tagtatagct attttctcat tattttcatc tacaccaaaa
    10981 gagactattt taaatactat atctttgtta tgtgactttc tagctacaat gtctcctacc
    11041 ttcatagtta aacctccaat aaatttttat cttagtttat actatgttcg atataaaact
    11101 ttgtttccta atgtcaaaaa aacatttctt ataatacttc ttttatcact cttaaagcat
    11161 ttttatagta tatcttttct atatcatctt cactaaaccc ttcttttttt agaggttcat
    11221 acagtttacc catctgagat atatcttcaa tctcaacttt tgaatctatt ccatcaaaat
    11281 ctgaccctag ggatacaaca tctattcctc caacatttac aatatgtttt atgtgtctaa
    11341 ccatatcttc aagtttactt tcacttttat cacttaaaaa taagtggaag aagtttatac
    11401 ctgtaactcc acctttattt gctaatactt taatcatatc atcagttaaa tttctagaat
    11461 gattcatcat tgctcttgaa ttagaatgtg tagcaattat tggtttagaa gaaatctttg
    11521 caatttcata aaatccacca tctgaaatat gagaaacatc aaccaacatt cccaattcat
    11581 tcatcttatg taccacttct cttccaaaat cagtaagccc tttttctcta tattctactt
    11641 tattatgagg aaagctcaat tcattcacat gattccaact tattgtcatc attctaaccc
    11701 ctaagtcgta aaatttcttt aaattttcta atttaccttc aagtactgca ccttcttcaa
    11761 tagataaaag agctgtaagt ttaccttctg attggttttt cataatttct tcatagttag
    11821 tagctaaagc tatttcatca ctattttttt tcatttcctc atgaaattta tttgccatac
    11881 tcatgcaata atcaaaagga tgttttactt cttcagtatc aacaaaaaga gcaaatgttt
    11941 gagctaatga atcccccttt ttcaatctgt ctatatcaac agaatattta ttagacttaa
    12001 gttcacttgt ttctacattt tccattaatt ttgctatagt atcacaatgt aaatcaataa
    12061 atttcatatt tataccccct gtatatttta tatttatatc taaaaataga aatagttgtc
    12121 cttatagaca actattgaca actatttttt aatattcttt ttaatattac ttaatcttat
    12181 tcttctttta agttaggtat tactttttta acttcttcaa tttgatgcca tgttttatct
    12241 atattatttt cactaatata tttttttaaa tcagttaaag atgtaaaatc tttaactgat
    12301 aaattgtcat catcatctgc aaatcttaaa ataaacatga gactccctta ctttccacaa
    12361 catttcttat atttttttcc gcttccacat gggcatgaat catttcttcc tatttttgct
    12421 tctttaacta ttgttttaga cttcttataa gcctttatta tttcttttct ttggtctgct
    12481 ggtagtatat cttcccaacc ttgtaaatta tatagccaat gagcctcaac agttaccata
    12541 ttgttatata atttttccca atctattttt atagaaactt ctttatcagc ttctacttct
    12601 tctaaaacta tatcttcttt taagctttca cttattccat ctatgaatcc catgaaaaat
    12661 tcattagtag ttccaaattt ttcagccaat tcacttactt taccttcaac aacatccatt
    12721 ttattattta atatttcatt gtatattcca gcttcaactt tcaaatattc ttcccagaat
    12781 tttacttctg cttcttgact ttcatgatta tcacttaaat ttctccattc agtatataaa
    12841 ctcatacttt ttcctcctaa gtatacctat atttttatag tttttatagt ataaaatctt
    12901 ttagttagat tggaagtttc ttctttttga ttataccata ttctatttaa agttaactct
    12961 tatattatat cataattaaa caattctttt aatactttta tagcatactc ttggtttata
    13021 aaataagggc ttgtcgcaat tgttattact ttggcttttt taattaagtt ttttatcttg
    13081 taaaatttga gttcaaatgg tatatagtcc atatcacttg aaaatatatc caaatctata
    13141 tcaagaacaa actctgattc tacttctaaa tcaaatccat acagactatc tattattatt
    13201 aattctgaaa atatgttata ttttaaagca ggttttataa aacttcccac atttaaaact
    13261 tcatttgtat acctaaatac atcttttaga ttatttacat ctacatcata gttctcaggc
    13321 tctctagtat ccttatgttg gtctacatgc actaacttgc atccttttgt aaattcaccc
    13381 tttaacaaag attttgccca aaaataaaat gcatggttat gattgtcaaa cacatatatc
    13441 tttttatttc ccaggacata ttcgaccata ttctccaatc cttttgcttt tatttcctta
    13501 ttttcatcta cttcattaaa taccacctca ttgcctaacc ttacatcatt taatgtacca
    13561 tttataagtt ttggaacgta tatgctctta ttttctcttt tatcatatga aaatacatta
    13621 tttcctactg gtttttctat ataaaatcca ctatattcat aattttccat aaaactttat
    13681 ttcctccatt gttatataaa tatataagtt ataattttaa aaattaaaat gcacttattg
    13741 taaatcatat tatctattat agaaaacatc ttatataact aaatataata tcttctctca
    13801 atatgtatta tacaataagt gcaaaatata tttatataaa atatactaca ttcctataac
    13861 aaaaccatca gcttcaattg gaaggtaact aactcttcta ttcaactcca cctttccaaa
    13921 aaatactacc caatatggtc taaaaatact ttcaatttta actatttcaa tctctgggat
    13981 tcctcccata aacctatgaa ttaatctcat agccatttta tttgcctttg cttttactct
    14041 tcgttcgtcc ttattactat actgcacaat attacaatcc acatttttta caatttcata
    14101 tgtaggaaaa tcttgtacta tactaccaga acctgtagaa ccattcacta aaatttttaa
    14161 ttcatttata tcattagaat atctcctatt cttgaaaatg gatttcgttt tgtgaatttt
    14221 aagtgtgata attttaaact ctatataatg aagtctaact tcatgagcat atttgttctt
    14281 taatattagc ttactaaata aagaattttt cccactttta gctatctcta tagcttcttc
    14341 ttttgtaatt cttataggta acgctcttat atccataaaa tcattctctt tcttattttt
    14401 ttcttgtcat ttttaaaact ctatatatat aatcaaaaaa tattacaatt acaactgcca
    14461 taactccata tggaattatt tttccaacta taatatcatt tctagtcaat ataccataaa
    14521 atataaatcc tgttattaaa aagtacaata atactagaaa accgtctact ccaagccatt
    14581 ttatttctaa atcttttttt accttagaca ctaatttaga catagcagtt tctcctttta
    14641 tagattattt tgtgtattta aattttcacc tattctatta ccaatcatcc atgcaataaa
    14701 tgaaaaaggt atagctatca caagaggctc taactcaaag ctctgaactg gaaatatttt
    14761 aaaataacca aaagtcactc cacctacaat cattcctaaa ataccaccca tagatgttct
    14821 tcttccattt ccaagaagcc cacctactat aggtacaaat gccgaaccca tccaaatact
    14881 tcctacaaat atacatgcat catatacatt ttcaaatcta aatgctacgc taagccccat
    14941 aactgctgct aaacatacac ctattctagt aaactttact aattgtttct gagataactc
    15001 tttttttgta attcttccat atatatcatt tgctaatgta gctccagctg ctagataata
    15061 gctatccaat gttgatatta tagaacctaa aattcctatt ataaatatta ctcttattcc
    15121 ttgtggtaat gtatctaaaa ctaaagttat atatccagcg gctggagcca tctctggata
    15181 agtagctctt actataagtc ctgttatagt taacaccatg tcaaaacata tccatagtga
    15241 aaaacaagtt aataaggctc ttcttccaac cttaggagaa tctgctgctg aaaatctttg
    15301 ataaaaagtt gggtctgaat aagctccaaa agaatataca attaacatca atgctttcat
    15361 agctgacata ccttttgtag ggtgcataag ctgtggtgca tctttgagtg cttctgtgag
    15421 accttgaaat cctcctattt gttcaaattg acctggcata accatagtta aaccaaatac
    15481 catacaaaaa aacaatacca tatctgtaat ggcaactccc ataagcccac caagtactgt
    15541 aagtacaacg actactccta ccattattaa tcctattaat tctttatcta ttttccatgc
    15601 tacgttacct atagttccca tagcagttat ttctccaagt tgtgcacaat atataaacat
    15661 caaaattgcc ccaattatag caacatgctt accataaaaa ctttccagta aatcaggaac
    15721 tgttatattg gttcttatat gaatccttgg accaacccac aatgctaaag gcattctccc
    15781 aatatgtgca gctattgacc aaactgccca tgcagatatt ccatatatag ccgcatactc
    15841 tacactacct actgtaccaa ctcctccata ccatgatgca accaaagttc ctactattaa
    15901 ggaccatggt aaacttcgat ttcctaggta ataactttcc atatctttta catttctaga
    15961 aaaataataa cctattccta ataatataaa tgcaaatata actataatcc cattttccca
    16021 aaatgttaac atatctaatc tccacattct tatattttaa tttttaactt taatatctaa
    16081 atatgaacaa ctctacccat ttccataaaa ataggtagag ttatataata attataagta
    16141 tttaagtaat cttgttatta agcaatttta ttgtttaaac ctttattttc tcttttattt
    16201 attttattta aagcataagt agcaaatatc cctataactg cacatactac agataaaata
    16261 aatatatatg tatatcctgt ttgtccatat ttatctaacc aattacctgc aatagtgtaa
    16321 taaaacgcat ctggagaaaa tcctataaat gaagcaaatc caactactgc tcctgtagtt
    16381 tctaaagata tattagactc tgaaacagat gcaaagtata tacctctaaa tccaaataaa
    16441 attacactta gtattatcat gtttattaca gctatccata taaatcctgt actcttagga
    16501 ataaccaagt aagcagctgt acttactgtc attaatataa atcctacaaa cattactctt
    16561 atagatgaac caactttatc tgttataaca ccagcaactg gagatgctcc catttttata
    16621 acataagttc ttattattga taaagcagaa actagagtca ttggcattac ataaacttct
    16681 gataagtatg gacttaaata agttaaactt gagaatacta aataagttga aaatattata
    16741 agaccaatta accaagcctt tggcattgta actactttta acaatgactt aatacttaat
    16801 ccttcatctg atgctccact atcttttttc tcttccacta agaaaaatag aagtatacca
    16861 catattatag atgctgcaga atataaccat actacatatt tgaatcctac tgttatttca
    16921 gcaaatttac caaagaagta aagacctata aaagaaatta acgtacctga tacacctgat
    16981 aacccttcat aaaaaccaaa tattcttcct tgttctgact cacttccttg cattctaaca
    17041 accttaacag atgctgagaa gaaagttaat attgttgtaa atccccataa tacaaatatt
    17101 aagagtatca tactatatga tgggaatgta gagaaataaa aaccaagtac tcctgaagca
    17161 attaaagaaa aagttatcag ctttctaacc gagaatctat ctgcaaacca tcctcctata
    17221 aaataagata ccattcctac taatccatat aaactcaata agttccctat ttgtgtatgg
    17281 ctcaatgata aagcttgttg cattgggtca taaaatgatg atttcatata tggtaaacta
    17341 tacatagccg ttgttccaaa tgccaatatg aatattatca agaatctttt aaaattactt
    17401 ttgttctcca ttaattttct ctccttaaaa ttttattgtt tataatctca ttcatgtaat
    17461 tttattttaa tttttttatt gcttgtttaa agtcatttat tatatcattt gaagattcta
    17521 atcctacagc tattcttatt agattttctc cattcatttg aactaatgta tgctcttccc
    17581 ctaagcttgt tgctattgtt gcaagctcta aagagttcat taattttcta ctagcttggt
    17641 ctcttgtcaa tccattgata ccatccttaa gtttaaatga aactacacct ccacctaaac
    17701 cattcatttg agttttacaa agctcatatt gaggatgaga ttttaatgct ggatacttta
    17761 ccaattccac attctcttct tcctctaaaa actctgctac tgctaatcca ttttcacaat
    17821 gctttttcat cctcatatct aacgttttca tacctcttaa aatcatccat gcactcattg
    17881 gtggtaaaga tgctcctgta aaacaacata aacttggcca tctaactaaa tctattaatt
    17941 cttttgaacc tgctactgct ccccctaaag tatccccatg tccatttata aacttagtaa
    18001 gactatgtat tactaaatct gcacctaaat ctattggtct ttgtacaatt ggtgtcccaa
    18061 aagtactatc aactatagct aagcttttat tttcatgagc aatttctgct attgttttta
    18121 tatctactaa gtctaatgat ggatttaaag gactttctat ataaactatt ttagtatttg
    18181 gtctcattgc ttcttttaca ttttgtggat ttactgcatc tacaaatgta acttcaactc
    18241 caaatttaga taacaactca gacatgaata atttagtgtg tgaaaatatt ccatctgaac
    18301 taactatatg gtcaccactt tttaccaatg ctaatatagt agatgttatt gcccccatcc
    18361 ctgatgtact agatatggca ctttctgcat tttcaagcat agccaactta gcttctaact
    18421 ctgtaagagt aggattacac tctcttgtat acacatatcc ccatgtcata cacaactctt
    18481 caaaatgctc tactgtcttt gcagcaaatg ttgctgtttg acatattggt gtagccaatg
    18541 cacctgttgt tgggtctgca ctatgtcccc aatgtattat ttttgtctct aaatcttgat
    18601 taatattgtt actcattttg atttcctccc attttgactt atttatacaa cgttttccct
    18661 gccaaattta atcaatttag cttagtctta atacattttt tcaagtatac ttttttattt
    18721 gttataattt ctaaaaatat gacacttatt ttgttttttt acagttctaa ttataaactc
    18781 tatagtaact atagagtcaa tatgtttttg atattttttt tacaatgttt aaaatagtca
    18841 gtacaaaaat aggtatatct tagaataact aaagtcatcc taagatatac ctatttttat
    18901 atcacaatat aaataattac atataagcat tagttttttt at
//
LOCUS       DAMJVX010000065        18717 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMJVX010000065 DAMJVX010000000
VERSION     DAMJVX010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18717
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.068"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            95..1696
                     /locus_tag="PC407_001999"
     CDS             95..1696
                     /locus_tag="PC407_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HDF2497445.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADRQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASKSNGQPPTITVGSTEIYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSYDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKNLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            1915..3504
                     /locus_tag="PC407_002000"
     CDS             1915..3504
                     /locus_tag="PC407_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HDF2497446.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQRNSIPSDTEKMLKDVKKV
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGSPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNIDTDKVGKYKVTIKATDIGGKTTSISVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYSLVNSYRKEKGKEPLQVSKNLEGLSNSWSKYMADKGQFSHVINGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDAREVANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILPNGEVYATQEFLNK"
     gene            3730..6006
                     /gene="slpA"
                     /locus_tag="PC407_002001"
     CDS             3730..6006
                     /gene="slpA"
                     /locus_tag="PC407_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HDF2497447.1"
                     /translation="MNKKNIAIAMSGLTVLASAAPVFAAEDMSKVETGDQGYTVVQSK
                     YKKAVEQLQKGLLDGSITEIKIFFEGTLASTIKVGAELSAEDASKLLFTQVDNKLDNL
                     GDGDYVDFLISSPAEGDKVTTSKLVALKNLTGGTSAIKVATSSIIGEVENAGTPGAKN
                     TAPSSAAVMSMSDVFDTAFTDSTETAVKLTIKDAMKTKKFGLVDGTTYSTGLQFADGK
                     TEKIVKLGDSDTINLAKELIITPASANDQAATIEFAKPTTQSGSPVITKLRILNAKEE
                     TIDIDASSSKTAQDLAKKYVFNKTDLNTLYRVLNGDEADTNRLVEEVSGKYQVVLYPE
                     GKRVTTKSAAKASIADENSPVKLTLKSDKKKDLKDYVDDLRTYNNGYSNAIEVAGEDR
                     IETAIALSQKYYNSDDENAIFRDSVDNVVLVGGNAIVDGLVASPLASEKKAPLLLTSK
                     DKLDSSVKAEIKRVMNIKSTTGINTSKKVYLAGGVNSISKEVENELKDMGLKVTRLAG
                     DDRYETSLKIADEVGLDNDKAFVVGGTGLADAMSIAPVASQLRNANGKMDLADGDATP
                     IVVVDGKAKTINDDVKDFLDDSQVDIIGGENSVSKDVENAIDDATGKSPDRYSGDDRQ
                     ATNAKVIKESSYYQDNLNNDKKVVNFFVAKDGSTKEDQLVDALAAAPVAANFGVTLNS
                     DGKPVDKDGKVLTGSDNDKNKLVSPAPIVLATDSLSSDQSVSISKVLDKDNGENLVQV
                     GKGIATSVINKLKDLLSM"
     gene            6242..8587
                     /gene="secA"
                     /locus_tag="PC407_002002"
     CDS             6242..8587
                     /gene="secA"
                     /locus_tag="PC407_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2497448.1"
                     /translation="MSVLDAILDKADEQEIKKLNLIVDKIESLEKDMEIKENEQLKEM
                     TNTFRLRLDKGESLDDILPEAFAVVREVSKRVLGMRQYKVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYEFLGLTVGVILSNQD
                     PSIRKEQYKCDITYGTNSEFGFDYLRDNMVPDLSHKVQRELNFAIVDEIDSILIDEAR
                     TPLIIAGDGDEDLKLYELANNFVKTVKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNRPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSKMLKK
                     QGIKHQVLNAKQHDKEAEVISKAGKLDAITIATNMAGRGTDISLGAGDKEEEEKVKNL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIKEDIQKMVKDIIQEAGETYLIGRKRDYYGYFKHLYSTFMPADTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMIGIDKVAELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
     gene            9002..10873
                     /locus_tag="PC407_002003"
     CDS             9002..10873
                     /locus_tag="PC407_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HDF2497449.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTDTVTSKTIEIGIGDEKIDLTANPVDSTGTNLDPSTEGFRVNKIVK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISS
                     KDSETGKYKFTIKYTDASGKAIELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADVDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKADITDSQRNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            10980..11687
                     /locus_tag="PC407_002004"
     CDS             10980..11687
                     /locus_tag="PC407_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2497450.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYKEGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDELAINKIQYVHAP"
     gene            11720..13555
                     /locus_tag="PC407_002005"
     CDS             11720..13555
                     /locus_tag="PC407_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2497451.1"
                     /translation="MKISKKIVSLLTMTFLTVTLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWKKSENAVLVNDNSIADALSATPFAKAKDAPILLTQSNKLDSRTKAELKRLGV
                     KNVYLIGGSIALSSEIEKQLNAENISFERISGNSRYDTSLKLAEKLDREKSISKIVVV
                     NGEKGLADAVSVGAIAAQENMPIILSDSENGTEVADNFIDSKDIEKSYVIGGTYSISN
                     SVERSLPNATRIAGSSRSETNAKIIEEFYKDTDIKNIYVTKDGTRSKHDLIDSLAVGV
                     LASKNGSPILLAGNKLDSSQKDVLNTKIIDKVIQIGGLGNENVVEDVIDMQEETKYTV
                     ETIDELNVAIKKSDANDIIRFKPEKDKNITNSFKIETNKAITIEFDGIYKQSITIDMP
                     NGDIKNFGDIDNDLVINNIKNETLVNEGSIQGIDVYDENGCKIENEDSGEIWLITILK
                     NSKDVYILNRGEITKISNNSSSVTIKNSGNINTISGSEEPSISGKKPITNDTDKDEDK
                     KARGLYPKVESCIPALKDYVRITISTKPESSKYKIYYRVVDEKPSAKYVGEKISTKGW
                     SMVTISNQSFIERAKDGSYIEAVEIDTSNNEVSRWGKSSITDDGI"
     gene            complement(13603..13998)
                     /locus_tag="PC407_002006"
     CDS             complement(13603..13998)
                     /locus_tag="PC407_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDF2497452.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIEIRFVFKEFLSFVSFRLLSGVVEMLIMYVMIDLLF
                     VNDVIVKIFTNIVVIVLNYLFSKMIIFKK"
     gene            14225..16636
                     /locus_tag="PC407_002007"
     CDS             14225..16636
                     /locus_tag="PC407_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HDF2497453.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMDLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISIGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNESAFNELN
                     TLLGK"
     gene            16933..18510
                     /locus_tag="PC407_002008"
     CDS             16933..18510
                     /locus_tag="PC407_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HDF2497454.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVENLEAKVDSIEKAIDSISQKVN
                     KIQDILDKIGFLKKFLS"
BASE COUNT         7751 a         2072 c         3469 g         5425 t
ORIGIN      
        1 atcatatata ctctataata tatggaaaat aaagagaaaa taaatagaaa ataatcacaa
       61 tataaatata aaatataaaa atcatcaggg ggatatgaaa atgaacaaaa aaatattatc
      121 attaggtcta gcagtatcat taatcttagt aaacttcaaa agtgtaaatg catcatcagt
      181 agtagaaaaa atatacggta aagatagata tgaaacagca gcaaagatag ctgatagaca
      241 gacttatgaa acagtaattt tagtaaatac agaaaaatca cttgcagatg gattaagtgc
      301 aagtggatta tcgggagcta caaaagctcc aatactattt acacaacaaa ataagatacc
      361 agcagacaca aatagatgtc taaaaaatat caaaaaagca tatataatag gtacagaaga
      421 tactataagt aaatcagtag aaaaagaact agattctaaa aatatagaag tcaaaagaat
      481 tggtggagaa gatagactaa aaacaagcta tttaatagct aaagaaatag caactataaa
      541 aaaagtggat aaagtactat taactaacgc atatagtgga gaagcagacg caatgagtgt
      601 atcatcagta gctactagag atggagctcc aatcatactt acagatggaa agagtgtgcc
      661 ttttgatgta aaaaatattc aatcatattg tataggttca gaggagataa tgagtaaccc
      721 tctagttaaa aatacaaact cagtaagaat agaaggaact gaccgatttg aaactaacaa
      781 aaatgtaata gactactttt ttaatagtgc agatggattc tatgtatcag atggatacca
      841 attagtagat gcaatagcag ctgcaccact tactaaaaac tctccaatgg tactagtaaa
      901 tgatggaagc gataaaactg tattagaagg agctaagaat ataacttctg taggtgaaat
      961 aaatgagaag gtaatacaac agtgtataaa tgcttcaaag tcaaatggac aaccaccaac
     1021 aattacagtt ggaagtacag agatatataa gggtgaaaag tttgacactg gcaagttaaa
     1081 catagtggct aaagataaca ctgggaaggt attaccaata gaagttgatg ggtttataga
     1141 tacaaataga gtaggtacat acatattgac attaaaagct acagacgaat ggggaaaaag
     1201 tacaggaaaa agagtagaaa taaaagtgtt ggatgataaa tcctatgatt acaacagccc
     1261 agaatttaaa aaaatggtat ctactgaaat gtataactta atcaattcct atagaaaaga
     1321 aaaaggcaaa gagcctttgg tagtatctag taggctagaa ggtatggcaa atgcatggtc
     1381 taagtatatg atggataaaa aagtatttgc acattatata gatggaaaaa atgctccaca
     1441 agtattttct gagtttggaa tgagaagtga ggaaaatata gcatatattt atatagattc
     1501 taagaacgtt caaacgacac aagatgcaaa aaacttggca aaggctatat ttgaagtgtg
     1561 gaagaagtct ccagagtaca atgcaaacat gcttagtgat gagttctata gtactggatt
     1621 tgggctttat atattatctg atggtcaagt gcatgctact caggagtttt taaatggcaa
     1681 tgaaggtagt ttgtaaaaaa ttccataaat cttgtttttt tgttaattta ctgatattat
     1741 aaaatgaaaa tcacatctaa agtacttttt gtaatctgtt ttgttattgc tttacaggct
     1801 atggtatttt aggtgttttt tgttttagta gtttatagat tttgagggaa tcactactga
     1861 ggatgtaatt tatgattagc tgaattatat aaaaaaatta gtaggaggag ttcgatgaag
     1921 gtaaataaga gagtattatc aataggattg acaatatcat taataatggc aggagcacca
     1981 aatatcaatg cactttcgag catagaaaag atacaaggga aagatagata tgagacagct
     2041 gccaaaattg cacagaaaca aacatatgaa aatgtagtat tagtaaatac agataataca
     2101 ctagctgatg gattaagtgc aagtggatta gcaggaacag ttaaagcacc tatattatta
     2161 tcacaaagaa acagtatacc atcagacaca gaaaaaatgt taaaagatgt aaagaaggtt
     2221 tatataatag gaactgaaga ttcaatagga aaatcagttg aaaatgaatt aaagcaaaaa
     2281 ggaatagaag ttaaaagaat aggtggtaat gatagaattg aaacaagtta tcttatagca
     2341 aaagaaatag cttcaataaa acctatagat aaggttttta taactaatgg ttatacaggt
     2401 gaagcagatg ctatgagtgc gtcatcagta gcatcaagag atggatcacc tataatttta
     2461 actaatggta aaaatgttcc atttgagaaa aaagaaggtg tgcaatgtta tgctcttggt
     2521 tcagaggaga taataagtaa tgatttagtt aaaaaaacta atgcagttag attagctgga
     2581 gaagatagat ttgagactaa taaaaaagtt ataaaacatt tttatagttc ggcaaaagaa
     2641 ttttatcttt ctaaaggtta tcaattagta gatgcagttg ctggttcttc tatagcaaaa
     2701 aatgcaccta ttgtattagt tgacggaaat agtgataagt ctgtacttag aagtgctgat
     2761 aagattacag cactaggagg aatagatgaa aagacactag aacaatgttt aagtgcatca
     2821 tcactggatg caagtgcacc aacaataact gttggaaatt taaatattta tcaaggtgat
     2881 aagtttgata taagtaaact gaatatcgta gcaaaggata gcaatggtaa cgatttgaca
     2941 ccagagctta taggaaatat tgatactgat aaggtgggta aatacaaagt tactataaaa
     3001 gctactgata ttggtggaaa gactacatca ataagtgtag aagtaaatgt acttgaatat
     3061 aagactaatg atatgaatag tagtgagttt aaaagaatgg tttctagtga aatgtatagt
     3121 ttggtaaatt catatagaaa agaaaaaggt aaagagccat tacaagtttc taaaaactta
     3181 gaaggacttt ctaattcctg gtctaagtac atggcagata aaggtcagtt ttcacatgta
     3241 ataaatggta agaaatctgt tgaagtattt actggatatg gcttgagaag tgaagaaaat
     3301 atagcttttg taccacttgt tacaaaaagt acttatacaa caaaggatgc tagagaagta
     3361 gcaaatgtaa tatttacagt ttggaaaaag tcagataaat ataatgaaaa tatgttaaat
     3421 agtgattttg tatatactgg gtttggatta tatatattgc ctaatggaga agtgtatgct
     3481 acacaagaat ttttaaataa gtaatttaaa taagtaaaat caaatttttt aagttattaa
     3541 atattggagt ttaagggtat aaaaactcat atgcttggga aaaataataa tttcaaaaaa
     3601 atgacttgca aaaagaataa aaatggatta ttatagagat gtgagaaata ttaggaatat
     3661 atggatgatt attctatgta cataatgaag agatgtaatt ttaatataat gttgggagga
     3721 atttaagaaa tgaataagaa gaatatagca atagctatgt caggattaac agtattagct
     3781 tctgcagcac ctgtgtttgc agcagaagat atgtcgaaag ttgagactgg tgatcaagga
     3841 tatacagtag tacagagcaa gtataagaaa gcagttgaac aattacaaaa agggttatta
     3901 gatggaagta taacagagat taaaattttc tttgagggaa ctttagcatc tactataaaa
     3961 gtaggagctg agcttagtgc agaagatgca agtaaattat tgtttacaca agtagataat
     4021 aaattagaca atttaggtga tggggattat gtagatttct taataagctc tccagcagag
     4081 ggagataaag taactacaag taaacttgtt gcattaaaaa atttaacagg tggaactagt
     4141 gcaataaaag tagctacaag tagtattatt ggtgaagtcg aaaatgctgg tactccggga
     4201 gcaaaaaata cagctccaag tagtgctgca gttatgtcta tgtcagatgt atttgataca
     4261 gcttttacag attcaactga aactgctgtg aaacttacta taaaagatgc tatgaaaact
     4321 aaaaagtttg gtttagttga tggaactact tattcaacag gtcttcaatt tgcagatgga
     4381 aaaacagaaa aaattgttaa attaggagat agtgatacta taaatttagc caaagaatta
     4441 ataataacac ctgcaagtgc aaatgatcaa gctgcgacta ttgagtttgc taaaccaaca
     4501 acacaatctg gaagcccagt aataactaaa cttagaatat tgaatgcaaa agaagagaca
     4561 atagatattg atgctagttc tagtaaaaca gcacaagatt tagctaaaaa atatgtattt
     4621 aataaaacag atttaaatac tctttacaga gtattaaatg gggatgaagc agatactaat
     4681 agattagtag aagaagttag tggaaaatat caagtggttc tttatccaga aggaaaaaga
     4741 gttacaacta agagtgctgc aaaggcttca attgctgatg aaaattcacc agttaaatta
     4801 actcttaagt cagataagaa gaaagactta aaagattatg tggatgattt aagaacatat
     4861 aataatggat attcaaatgc tatagaagta gcaggagaag atagaataga aactgcaata
     4921 gcattaagtc aaaaatatta taactctgat gatgaaaatg ctatatttag agattcagtt
     4981 gataatgtag tattggttgg aggaaatgca atagttgatg gacttgtagc ttctccttta
     5041 gcttctgaaa agaaagctcc tttattatta acttcaaaag ataaattaga ttcaagcgta
     5101 aaagctgaaa taaagagagt tatgaatata aagagtacaa caggtataaa tacttcaaag
     5161 aaagtttatt tagctggtgg agttaattct atatctaaag aagtagaaaa tgaattaaaa
     5221 gatatgggac ttaaagttac aagattagca ggagatgata gatatgaaac ttctctaaaa
     5281 atagctgatg aagtaggtct tgataatgat aaagcatttg tagttggagg aacaggatta
     5341 gcagatgcca tgagtatagc tccagttgca tctcaattaa gaaatgctaa tggtaaaatg
     5401 gatttagctg atggtgatgc tacaccaata gtagttgtag atggaaaagc taaaactata
     5461 aatgatgatg taaaagattt cttagatgat tcacaagttg atataatagg tggagaaaac
     5521 agtgtatcta aagatgttga aaatgcaata gatgatgcta caggtaaatc tccagataga
     5581 tatagtggag atgatagaca agcaactaat gcaaaagtta taaaagaatc ttcttattat
     5641 caagataact taaataatga taaaaaagta gttaatttct ttgtagctaa agatggttct
     5701 actaaagaag atcaattagt tgatgcttta gcagcagctc cagttgcagc aaactttggt
     5761 gtaactctta attctgatgg taagccagta gataaagatg gtaaagtatt aactggttct
     5821 gataatgata aaaataaatt agtatctcca gcacctatag tattagctac tgattcttta
     5881 tcttcagatc aaagtgtatc tataagtaaa gttcttgata aagataatgg agaaaactta
     5941 gttcaagttg gtaaaggtat agctacttca gttataaata aattaaaaga tttattaagt
     6001 atgtaatata aatataataa tataaaaagg cttcttatat gagaagtctt tttatattat
     6061 gttttcgaat acaaaaaaag agactaaatt tagtctcttt tttatgtgag aaatacttaa
     6121 atatataaag atgtaattta tttaagtatg attattatac tatacaataa aataattgtc
     6181 tactattcta taattttaag gtataataat aattgtatga taaaagagga ggtcaaaaaa
     6241 tatgtcagtt ttagatgcaa tcttagataa agcggatgaa caagaaatta aaaaattaaa
     6301 tttaatagta gataaaatag aatctttaga aaaagatatg gaaatcaaag agaatgaaca
     6361 attaaaagaa atgacaaata cttttagact tagattagac aaaggtgaaa gtttggatga
     6421 tatacttcca gaagcatttg ctgttgttag agaggtctca aaaagagttt taggaatgcg
     6481 tcaatataaa gttcagttaa ttggaggtat agtaattcat caaggaaaga tagctgagat
     6541 gaagactggt gaggggaaaa ctttagttga ggtagctcca gtatatctaa atgctttaac
     6601 aggaaaaggt gtacatgtaa ttacagtaaa tgattatttg gcagaacgtg ataaagaact
     6661 tatgcgtcca gtatatgaat ttttaggatt gactgtaggt gtaattttat ctaaccaaga
     6721 cccaagtata agaaaagaac aatataaatg tgatataact tatggaacaa atagtgaatt
     6781 tggatttgac tatttaagag ataatatggt gccagatttg tctcataaag tacaaaggga
     6841 actaaacttt gccatagtag atgagataga ctcaatatta atagatgaag ctagaactcc
     6901 acttattatt gcaggagatg gtgatgaaga tttaaaactt tatgagttag caaataactt
     6961 tgtaaaaact gttaaagaag aagactttga gatggataga aaagataaaa ctatagcatt
     7021 aacagcaagt ggtataagca aagctgagtc attttttggt ataacaaacc ttactgatat
     7081 aaagaatata gaattatatc atcatataaa tcaagcttta agaggtcata agcttatgga
     7141 aaaagatgtt gactatgtta tctcaaatgg agaagtaatg atagttgacg aatttacagg
     7201 aagagtaatg gatggtagaa gatatacaga tggacttcac caagctatag aagccaaaga
     7261 aggtgttgag ataaagaatg aatctaaaac tatggctact gtgacttatc aaaatttctt
     7321 cagactatat gaaaaacttt ctggtatgac tggtactgca aagacagaag aaggggaatt
     7381 tgagtcaatc tataaattaa atgttgtcca aataccaact aatagaccag ttattagagc
     7441 tgatttgcat gataaggtat ttaaaacaga agaagaaaag tatagtgctg ttgtagaaga
     7501 aataataagg atacataaga ctagacagcc aatactcgtg ggaacagttt ctgttgaaaa
     7561 atctgagaag ttatctaaaa tgcttaagaa gcaaggtatt aagcatcaag tcttaaatgc
     7621 taaacaacat gataaagagg cagaggtaat ttctaaagct ggtaaattag atgctataac
     7681 gattgctaca aatatggcag gtagaggtac ggatatttct ctaggtgcag gagataaaga
     7741 agaagaagaa aaagttaaaa atttaggtgg tctttatgtt ataggaacag aaagacacga
     7801 atcaagaaga attgacaatc agcttagagg gcgttctggt cgtcaaggag acccaggtac
     7861 atcaagattt tttgtaagtc ttgaagatga tgtaataaag ctttatggtg gaaaaactat
     7921 agagaaactt atgaagagaa caagttcaaa ggaaaatacg gctattgaaa gtaaagcact
     7981 tacaagggct atagaaagag cacaaaaagg tgtagaaggt aagaattttg aaataagaaa
     8041 aaatgttctt aaatatgatg atactattaa tgagcaaaga aaggttatat ataatgaaag
     8101 aaataaagtg ttaaatgatg aagatataaa agaagatatt caaaaaatgg ttaaagacat
     8161 catacaagaa gcaggagaaa cttatttaat tggaagaaaa agagattatt atggatattt
     8221 taaacattta tatagtacat ttatgcctgc agatacatta ctaatacccg gtgtagataa
     8281 aaaaagtgtc caagagataa ttgatagcac atatgaaatt tcaaaaaggg tttatgactt
     8341 gaaaaagatg atgattggta ttgataaagt tgcggagtta gaaaaaacag tacttttaaa
     8401 agtggttgat caatactgga tagatcatat agatgctatg gaacagttaa gacagtatat
     8461 aggtcttaaa tcttatgctc aaaaagatcc gtttaaagaa tatgctttag aaggatatga
     8521 tatgtttgaa gctttaaata aaaatataag ggaagcaaca gtacaatact tatataaatt
     8581 taactaaaaa agttcatgta atttttatta aatgaaaaat aaaatatttt gattctttat
     8641 attgtaatta caattaaata attaaggaat acaaatgaaa aagaagctga aaatcagctt
     8701 ctttttctgt gtgagaaata cggaaaaaag agatgcttga aatttctttc ttccatttaa
     8761 tattatacta taaagaagat gaaaagtcta ttagttataa actggattat taaaaaaaat
     8821 tacaattttg ttactttttt gtatacataa atattaaaaa aataaaatat aagaaaaagt
     8881 ttatatcttt tggttaatta ttacaataag tctcatttat tgaaataata tcaaatatat
     8941 attataattg gtaaataagg aaaaaataat aaaaatttga attttttagg gggaaaatac
     9001 catgaataaa aaaaatcttt ctgtaattat ggctgctgca atgataagta catcagtagc
     9061 tccagttttt gctgcagaaa ctacacaggt aaaaaaagaa acaataacta agaaagaagc
     9121 tacagaacta gtttcgaaag ttagagattt aatgtctcaa aagtatactg gtggttctca
     9181 agttggacaa ccaatatatg aaataaaagt tggcgagact ttatcaaaat taaaaataat
     9241 aactaatata gatgaattag agaaattagt aaatgctttg ggagaaaata aagaacttat
     9301 tgtaactata acagataaag ggcatataac aaatagtgca aatgaagtag ttgcagaagc
     9361 aactgaaaaa tatgaaaatt cagcagacct ttccgctgag gctaattcta taacagaaaa
     9421 agctaaaact gaaactaatg gaatttataa agttgcagat gtaaaagctt catatgatag
     9481 tgctaaagat aagttagtta taactttaag agataaaaca gacacagtaa cttctaaaac
     9541 tatagagata ggtattggtg atgaaaaaat tgatttaaca gcaaatccag ttgattcaac
     9601 gggaacaaac ttagaccctt ctacagaagg atttagagta aataaaatcg ttaaactagg
     9661 tgtagcagga gctaaaaata ttgatgatgt ccaattagct gaaataacta taaaaaatag
     9721 tgacctaaat acagtttcac cacaagattt atatgatgga tatagattaa ctgttaaagg
     9781 taatatggta gcaaatggaa catcaaagtc aattagtgat atttcatcaa aagattcaga
     9841 aacaggaaag tataaattta ctattaagta tactgatgca tctggaaaag caatagagct
     9901 tactgtagaa agtactaatg aaaaagattt aaaagatgcc aaagctgcat tagaaggtaa
     9961 ttcaaaggtt aaattgatag ctggagatga tagatatgca actgcagtgg ctatagcaaa
    10021 acaaacaaaa tatactgaca atatagttat agttaattca aataaactag ttgatggatt
    10081 agcagctaca ccacttgctc aatctaaaaa agcacctata ttattagcat ccgataatga
    10141 aataccaaaa gtaactttag attatataaa agatataatt aagaaaagcc catcagctaa
    10201 aatatatata gtaggtggag aatcagcagt atcaaataca gctaaaaagc aattagaatc
    10261 agtaactaag aatgttgaaa gactagctgg agatgataga catatgactt ctgtagcagt
    10321 agcaaaagct atggggtctt ttaaagatgc atttgtagta ggtgcgaaag gggaggctga
    10381 tgctatgagt atagctgcca aagctgctga acttaaggct cctataatag taaatggctg
    10441 gaatgatctt tcagcagacg ctatcaaatt gatggatgga aaagagattg gtatagttgg
    10501 tggttctaac aatgtatcta gtcaaattga aaatcaactt gctgatgttg ataaagatag
    10561 aaaagttcaa agagttgaag gagaaacaag acacgatact aatgctaagg ttatagaaac
    10621 atattatgga aaattagata aactatatat agcaaaagat ggatatggaa ataatggtat
    10681 gctagtagat gcattggcag caggacctct agcagcaggt aaaggtccaa tacttctagc
    10741 taaagctgat ataacagact cacaaaggaa tgcacttagt aaaaaattaa atcttggtgc
    10801 agaagtaact caaataggta atggagttga attgacagta atacaaaaga tagctaaaat
    10861 actaggttgg taataactta tactgtggag aagattcctt aattaataca ggaatctttt
    10921 tctataatta tgctatttat tgtaaattat aacattttat aaagtaggag gatgtagtag
    10981 tggtagatgc aactttattt tttacaccac atcaagatga tgaaacctta agtatgggaa
    11041 gtgctataat tgaacatgtt gaaaaaagtg atacacatgt catattatgc acagatggta
    11101 gtaaatctat aataagaaaa gttttggatg atggagggtc atgttcttat catattaaag
    11161 atgttcataa atactcttta tcagaaagtg aattttcaaa agatagagat gaagaattta
    11221 aggatagctg tgaagcaatg ggtgtaaaag aaagtaatat acatatagaa gataatagag
    11281 cacatgatgg tgagttaagt aaagaaaagg caagggaaat aatacttaag tacttagaag
    11341 aatatccaga tgcaaaagta aaaactgtta ctccgtttaa agctagtgga atacatgaag
    11401 accatagggc attaggagaa gctgcactag agttatataa agaaggcaaa ataaaagatt
    11461 taagatttta tgtagaacca tacgactata aggattttaa aaaagttaat ccaaatgtag
    11521 aggtatggaa ggtattacct agtcaagagg agaagttatt aagtgcaatg aatgcatata
    11581 aaaaatggaa tcctgaaagt ggacattatg ctataggata tcattcagta aaatctcatt
    11641 ttgatgagct agctataaat aaaatacagt atgtacatgc tccataaaat attatgaaag
    11701 aattaggagg taagaattaa tgaaaatatc aaaaaagata gtgtctttgt taactatgac
    11761 atttttaact gttacattat atggaaatac atctaatgca tctacaaaag atacattaac
    11821 aggttctgga agatgggaaa cagcaataaa aataagtcaa gcaggatgga aaaagtctga
    11881 aaacgctgtg ctagtaaatg ataattcaat agcagatgct ttatcagcta ctccatttgc
    11941 taaagcaaaa gatgcaccaa tattattaac tcaaagtaat aaattggata gtagaacaaa
    12001 agcagaatta aaaagacttg gtgtgaaaaa tgtatattta ataggtggtt caattgcatt
    12061 aagttcagag attgaaaagc agttaaatgc agaaaatata agttttgaaa gaatatctgg
    12121 aaatagtaga tatgatactt ctttaaaatt agctgaaaag ctagataggg aaaaatctat
    12181 atctaaaata gtagtagtaa atggagaaaa gggtcttgct gatgcagtga gtgttggagc
    12241 tatagctgct caagaaaaca tgccaataat actttctgat tcagagaatg gaactgaagt
    12301 agctgataat tttatagata gtaaagatat agaaaaatcg tatgtaatag gtggtacata
    12361 ttctatttct aattctgtag aaagaagttt accaaatgca acaagaatag caggtagtag
    12421 tagaagtgag acaaatgcaa aaattataga agaattttac aaagatactg atataaaaaa
    12481 tatttatgta acaaaggatg gtacaaggag taagcatgat ttaatagatt ctttagctgt
    12541 gggtgtatta gcttctaaaa atggttctcc aatactttta gcaggaaata agctagatag
    12601 ttctcaaaaa gatgtgttaa acactaaaat tatagataaa gttattcaaa ttggcggatt
    12661 gggtaatgaa aatgttgtag aagatgtaat agatatgcaa gaagaaacta agtatactgt
    12721 tgaaactatt gatgaactta atgttgctat aaaaaaatca gatgcaaatg acatcataag
    12781 atttaagcca gaaaaagata aaaatataac taattctttt aaaatagaaa caaataaagc
    12841 tataactatt gaatttgatg gtatatataa acaatcaata actatagata tgccaaatgg
    12901 agatataaaa aactttgggg acatagataa tgatctagta ataaataata taaaaaatga
    12961 gactttagtt aacgaaggaa gtatacaagg gatagatgtt tatgatgaga atggatgtaa
    13021 aatagagaat gaggattctg gagaaatatg gcttataacg atacttaaaa attcaaaaga
    13081 tgtatatatt ttgaatcgtg gagaaataac taaaatatct aataattctt ctagtgtaac
    13141 aataaaaaat tcaggtaata ttaatactat aagtggaagt gaagaaccat caataagtgg
    13201 taaaaaacct ataactaatg atacagataa agatgaagat aaaaaggcac gtggtttata
    13261 tcctaaagta gaatcttgta tacctgcttt aaaagattat gtaagaataa ctatatcaac
    13321 aaaaccagag agttcaaagt ataaaattta ttatagagta gttgatgaaa aaccatctgc
    13381 gaaatatgtt ggagaaaaaa ttagtactaa aggttggagt atggttacaa tatcaaatca
    13441 atcttttatt gaaagagcca aagatggatc atatatagaa gcagtagaaa tagatacctc
    13501 aaataatgaa gtttcacgat ggggaaaatc tagcattaca gatgatggaa tttagacatt
    13561 aaacttattc attttgcaga ttactattta gtaatctgct atttattttt taaaaattat
    13621 catcttgcta aacaagtaat ttaaaactat aacaactata ttagtaaata tttttacaat
    13681 aacatcattt acaaaaagca aatcaatcat tacatacata attaacattt caacgacacc
    13741 agaaagtagc ctaaaactaa caaaagacaa aaattcttta aatacgaacc ttatttcaat
    13801 tcttttactt tcaaatacaa aaaacttatt agtaacataa gcaaataaaa cagcaagtat
    13861 ccatgccaaa gcatttgcaa ccataaaatt aaataaaata actctagtaa aaaataaata
    13921 cgaaactata tttacaagag tagtaaatgc tccaaaaaat aaatataata ttgtctctct
    13981 atgctttttt aaaatcaaat ataatcctcc taatctaatt taatataaat atacttaaag
    14041 atactaacaa acattagtat agcacatcta ttaataaatc aatatatctt aaaatattat
    14101 aaaaataaaa ctataatatt ctataattaa tatgtactca taatccatat tttatcaaaa
    14161 taaaatatga ccttaattta gaaatctgat ataataaaaa ttaaatacat ataagggggt
    14221 aaacatgaga aagtacaaat caaaaaaatt gtcaaagcta ctggcattgt tgacagtttg
    14281 ctttttaata gtgtcaacaa tacctgtctc agcagaaaac cataaaactc tagatggagt
    14341 agaaactgca gagtattctg aaagttatct tcaataccta gaagatgtca aaaatggaga
    14401 cacagcaaaa tataatggag taataccttt tccacatgaa atggaaggta caacacttcg
    14461 aaataagggc agaagtagtc ttccatcagc atataaatca agtgtagcat acaacccaat
    14521 ggatcttggt cttactacac cagcaaaaaa tcaaggaagt cttaatacat gctggtcttt
    14581 ttcaggtatg tcaactttag aagcatatct taaattaaag ggatatggaa cgtatgactt
    14641 atcagaagaa catttaagat ggtgggcaac tggtggaaaa tatggatgga acttagatga
    14701 tatgtcaggt agttcaaatg taacagccat aggatatctg acagcatggg caggtcctaa
    14761 gttagaaaaa gatataccat ataatcttaa atctgaggca caaggtgcaa ctaaaccttc
    14821 aaatatggat actgcaccta ctcaatttaa tgtaacagat gttgttcgtc ttaataaaga
    14881 taaggaaact gtaaaaaatg ctataatgca atatggttca gtgacatcag gttatgcaca
    14941 ttattcgaca tattttaata aagatgaaac agcatataac tgtacaaata agagggctcc
    15001 attaaatcac gctgtagcga tagtaggatg ggatgataat tattcaaaag ataactttgc
    15061 atctgatgta aaaccagaat caaatggagc atggttagta aaaagcagtt ggggagaatt
    15121 taattctatg aaaggattct tctggatttc ttatgaagat aaaactctac taacagatac
    15181 agataactat gcaatgaaat cagtatcgaa accagatagt gataaaaaaa tgtaccaact
    15241 tgaatatgct ggtcttagta agataatgtc aaataaagta acagcagcaa atgtatttga
    15301 ttttagcaga gattctgaaa aacttgactc tgttatgttt gaaacagact ctgtaggagc
    15361 aaaatatgaa gtatattatg caccagtagt aaatggagtt cctcaaaaca actcaatgac
    15421 aaaacttgca agtggaacag tatcatattc tggatacata aatgtaccta ctaattctta
    15481 cagcttacca aaaggtaaag gagcaatagt agtagttata gacaacacag caaatcctaa
    15541 tagagaaaaa tcaactttag catatgaaac tgatatagat ggatactatc tatatgaggc
    15601 taaagcaaac ttaggtgaaa gctacatact tcaaaacaat aagtttgaag atataaatac
    15661 atatagtgaa ttttctcctt gtaactttgt tataaaagct ataacaaaaa catcttctgg
    15721 acaagctact tcaggagaat ctttaactgg agcagataga tatgaaacgg cagttaaagt
    15781 tagtcaaaaa ggatggactt cttcacaaaa tgcagtatta gtaaatggag acgcaatagt
    15841 ggatgcttta acagctacac catttacagc agcaatcgac tctccaattc ttttaacagg
    15901 aaaagataac ttagattcaa aaactaaggc agagttacaa agattaggaa ctaaaaaagt
    15961 ttatctaata ggtggagaaa attctttaag caagaatgta caaactcaac ttagtaatat
    16021 gggtatatca gtagaaagaa tttcaggtag tgatagatac aagactagta tatctctagc
    16081 tcaaaagtta aatagtataa aatctgtttc acaagttgca gtggcaaatg gtgtaaatgg
    16141 acttgcagat gcaataagta ttggtgcagc agctgctgat aacaatatgc caataatact
    16201 tactaatgaa aagagtgagt tgcaaggtgc tgatgagttt ttaaattcat caaaaataac
    16261 taagtcttat ataattggtg gtacagctac tttatcatca aatttagaaa gtaagctttc
    16321 aaatccaaca agacttgcag gaagtaatag aaatgaaact aatgctaaga taatagataa
    16381 attctaccct agctcagatt tgaaatatgc ttttgttgtt aaagatggtt caaagagtca
    16441 aggagacttg atagatggac tagcagtagg agcattaggt gctaaaactg attcaccagt
    16501 agttctagtt ggaaataagt tagatgaaag tcaaaaaaat gtacttaagt ctaagaaaat
    16561 agaaactcct attagagttg gtggaaatgg aaatgaaagt gcttttaatg aactaaatac
    16621 tcttttagga aaatagtgaa tggaaagata gtaaggatat acattttatt attaaaaaat
    16681 attcaataga agtttataaa tataaaataa caaaaagggg tgtctcaaaa tgaacttttt
    16741 agttcatgag gcactctttt ttgttgtttt atatttttat catttaaata atgaaaaata
    16801 ggcttgtcta tatttttaga tagttttctt attattgatg aaaaatgtaa taattttaaa
    16861 ctgtaaaata gtgtaaagtt attttataat ataaatatag taagatttta aacaaaataa
    16921 gggggaataa taatgaaagc accaaaaact attttaacaa tactgacaat agcgcttact
    16981 ttaagtagca tttctataat accatcatat gcacttacag aggaaaaatt aataggtaat
    17041 ggtagatatg aaactgctgt aaagataagt caaaaggcat acagttctag ttctaatgta
    17101 gtgttagtta atgacaattc attagctgat gcactttcag ctacaccatt tgcaaaggct
    17161 aaaggagcac caatactttt gacagaaagt gataagctag atgataggac tgaaaaagag
    17221 ataaaacgtc taggagctaa agatatttat ttgataggag gtactgctgt acttaataaa
    17281 gatatagaga acaagttaaa aggtaatgga cttaatgtag aaagaattaa tggtaaggat
    17341 agatatgaaa cttcactgat tttggctaat aagcttaagg atataaaaga tattaaggaa
    17401 gttgctgtag taaatggtga aaaaggatta agtgatgctg ttagtgttgg agcaccagca
    17461 gctcaaaata agatgcctat aattttgtct agtcctaagg acggagtaga agcatttgat
    17521 aaatttataa gagatgagaa agttataaaa gcatacgtaa ttggaggaac aaattctgtt
    17581 tcaagagctg tagaaaagag ccttcctaat gcagaaagag tgagtggaaa agatagaaat
    17641 gagactaatg ccaaagttat agaaaagttt tatactgata caaatttaag taatttatat
    17701 gtaactaagg atggtagtaa aaatgagaat caactaatag actcgttagc tgttggagta
    17761 ttggctgcaa aaaatgagtc tcctgttgtt ttggttggta ataaattaaa cacgaaacaa
    17821 agggatatac ttagtactaa aaaattaaat actataacac aggtaggtgg aaatggaaat
    17881 gaagaagctt ttgatgaaat taagagttta caagaaaaaa ctgtgtttga agcaaaaact
    17941 gttgaggaat taactgatat gataaatata gcaagtccta atgatattat aaactttaaa
    18001 ccaaaagaga atactgtaaa tgaagctttt agaatggtaa caaataaacc aataactgtg
    18061 aatataaagg gtgattgttc aaagactctt acagtagata tgcctaatgg tgaagtgaat
    18121 aactatgcta ctttggtaaa tgttatagtt agaaatattg gagaaggtgg atttaataac
    18181 catgatacaa tcactatatt gtcagttcgt gacaaaaatg gtagagttat agaaaatact
    18241 agaaattcag atatagatac gttgatgata ttagcttctg ctaatgatac aaaattgatt
    18301 aatgatggat atataggaaa gcttatagat aattcatcta atagtgatat aactaataat
    18361 ggtactatag ataaaaaagt taatcaagtt gagaatttag aggctaaagt agattcaatt
    18421 gaaaaagcta tagattctat aagtcaaaaa gtcaataaaa tacaagatat actagataag
    18481 attggatttc taaagaaatt tcttagttaa gaatatgtat atttaagtga agtgtgtgtt
    18541 gtaattgttc taaagttata tagttaaaca ggtttatata caaacagact tatagataaa
    18601 tatgtagttt agtatatatt tgaataagct ttggggaggt aacatgggga ttggaattga
    18661 atgtaacaaa aaaataaata aaagtattga caaaactaga ataaagaaaa ataatct
//
LOCUS       DAMJVX010000066        18666 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMJVX010000066 DAMJVX010000000
VERSION     DAMJVX010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18666
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.069"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..965
                     /locus_tag="PC407_002009"
     CDS             <1..965
                     /locus_tag="PC407_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HDF2497455.1"
                     /translation="TKLSMKYEILEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIK
                     ENKSFVSQAWTEKFGYNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNIHSQAV
                     YRLKKSNGEYIWCRTSITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTN
                     IYNKGTFEKLVEETIVMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQ
                     ASFGNKDLVGRIGGDEFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDSSVSI
                     GIARYPQDGTSFFELFKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(1655..2485)
                     /locus_tag="PC407_002010"
     CDS             complement(1655..2485)
                     /locus_tag="PC407_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2497456.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPITYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKI
                     "
     gene            2558..4285
                     /locus_tag="PC407_002011"
     CDS             2558..4285
                     /locus_tag="PC407_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2497457.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLIMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFVKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNREHFEKKRF
                     DRTAIDYSETAISINKVFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALI
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGRKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDVKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEIKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            4322..6184
                     /locus_tag="PC407_002012"
     CDS             4322..6184
                     /locus_tag="PC407_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2497458.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKVKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRRAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFSSEYDEEVV"
     gene            6517..7206
                     /locus_tag="PC407_002013"
     CDS             6517..7206
                     /locus_tag="PC407_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HDF2497459.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNNCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSIDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            7311..7997
                     /locus_tag="PC407_002014"
     CDS             7311..7997
                     /locus_tag="PC407_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HDF2497460.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKYGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            8014..9921
                     /locus_tag="PC407_002015"
     CDS             8014..9921
                     /locus_tag="PC407_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HDF2497461.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLTSVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETHKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDKKEVNAELTSSVVKT
                     NTDNETENKTTNDSESKNNTDKDKSGNDNNQKLEGPRGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            10374..11063
                     /locus_tag="PC407_002016"
     CDS             10374..11063
                     /locus_tag="PC407_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497462.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            11499..12038
                     /locus_tag="PC407_002017"
     CDS             11499..12038
                     /locus_tag="PC407_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2497463.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            12057..13100
                     /locus_tag="PC407_002018"
     CDS             12057..13100
                     /locus_tag="PC407_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2497464.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            13102..13938
                     /locus_tag="PC407_002019"
     CDS             13102..13938
                     /locus_tag="PC407_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2497465.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            14043..14717
                     /locus_tag="PC407_002020"
     CDS             14043..14717
                     /locus_tag="PC407_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2497466.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            14717..15769
                     /locus_tag="PC407_002021"
     CDS             14717..15769
                     /locus_tag="PC407_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2497467.1"
                     /translation="MIKFKKTISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            16213..17061
                     /locus_tag="PC407_002022"
     CDS             16213..17061
                     /locus_tag="PC407_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2497468.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            17051..>18666
                     /locus_tag="PC407_002023"
     CDS             17051..>18666
                     /locus_tag="PC407_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2497469.1"
                     /translation="MMFKIFNKSLLKLVIVLSIVLTISNFSLFSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINENYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKVTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKYISNISKILIFIVSISVLIFV"
BASE COUNT         7302 a         1903 c         3237 g         6224 t
ORIGIN      
        1 aaactaaact atctatgaaa tatgaaatac ttgaaaaaga aataattcat aaggaagaat
       61 taagacttag taatgagtgt tataaaatta ttgttgaaca gactgattct gtcgttttcg
      121 aatggaatat caaagaaaat aaatcatttg tatcgcaggc ttggactgaa aaatttggat
      181 ataacaatgc atgtaagaat atttttaaag aaattaaaga taaagactta gttcattctg
      241 aagataaagc tatatttgaa ggttttcttg aaagtattaa aaaaaagaat atacacagtc
      301 aagcagtata tcgtttaaaa aaatctaatg gtgaatatat ttggtgtaga acatcaatta
      361 caagcattta taatgatgaa aatgaacttt taagagttgt aggagtaatt gtagacgtag
      421 atagtgatat aaaaaaatat gaagagttaa gaactagagc agaaagtgac tcattgacca
      481 atatatacaa taaaggtaca tttgaaaaat tagtggaaga aactatagtt atgaatacag
      541 gtgataaaaa agatgcatta ttcattattg atttagatga ttttaaagaa ataaatgata
      601 attttggtca tccatttggt gattttgtac taaaaacatt tgctgataag atacaagcta
      661 gttttggtaa taaagactta gtcggaagaa ttggtggaga tgaatttgtt gtatatatgc
      721 aagattatgt tacagaagta aatttgcata aaaaagctaa agaattaaat agagtacttt
      781 cggataatta cactgatttg agttttagtt ttgattcttc tgtcagtata ggtattgcaa
      841 ggtatcctca agatggaact tcattttttg aattgtttaa aaatgcagat agagcacttt
      901 atagcataaa agcatcaggt aaaaattcat attgtttatt tgaagaagaa ctatatgtac
      961 agtgaatttg aatttagatt ttatgtgaga gtaagattta tatatacttg tggctgtctg
     1021 atttataaaa tatgttactt tgtatatttt tatatgtttc agtaaggtta attaacataa
     1081 gcaaaggtgt aagtaaattt atattagttt aagtttactt acacctttat aaaaataaat
     1141 tttgtaaatt taaacagttg gttttataag gatttttatt tattttaata tcttagatat
     1201 tcctttttgt attccttaga gaaaataaat aatgaaagga gtacagaaaa aattaaacat
     1261 cctaatatac cacttgaatt aagtcttaaa gcattaaagt ttaaaaaagc attatttaaa
     1321 tcggtatcca aaagaaaagg atgctttgat ttgctatata tggaaaaatt aaaaatatat
     1381 tcataatcaa atttataaaa ttaatatctt tccatcctaa cattacattt acttagatta
     1441 attattatta taaaaataaa aatacaaata atagattcat ttacattctt aaatgattaa
     1501 agctctttac cctttaaaca tgcataacat atcgttataa ttccagtaaa taagactaaa
     1561 atgcccattc ctaaaattgc agtttcatca tctattactc caaatgcttt taaagtaact
     1621 tatttcgctt tgtataataa taatttgtct ctttttatat tttaacttct gctgagattt
     1681 gagttagata ttcgttagaa tctcgcacac ttatatcata tagaagatat tcttcataaa
     1741 caaaatcccc aatacgtaaa ttattctgat taaaaaaatt taataacttt tcataagcct
     1801 tgtaaatagt ttcataacta cctttatgat aagcagttac ataaagtccc tttggtttga
     1861 ttgatacagg atatttcttg tactctttat taacttttgt aaataaatat gaatatgatt
     1921 caaagtcttt attaattata tcttcacctt taatgattga gccaatcgta ccatagtcac
     1981 tcaattcata gttgtccata aagttaatac attcatataa aaaattttct tcctcatctc
     2041 ctaaaaactg ttctttatat gtaataggtg ttttgaccaa gtactcttcc tcatgttctt
     2101 ttaaagtaat agtattcgca tctatattac aggcattttt agtgaatgaa atagtagatt
     2161 ccattagagc ttgaatatta tttagttttt caatttcatt tttaatatca atggttttct
     2221 tttctaaaag ctcaattagt aaattaggtg ttcttttgtc tatataagct ttaatttctt
     2281 ttaaaggcat ttttacttcc ctaagtgaag aaattatacc aaaaatgcta agttgatggt
     2341 atgagtagta tctatatcca ttttctttct ttaattctgg tgagaaaaga cctatatcat
     2401 cataatggaa aagtgttttt ttattgattc cacaaatctt tgcaaattct ccagttgtaa
     2461 aataattttt tgaaacttga ctcataatta taattcctcc ttgactatcc tgttacagta
     2521 tagtttatca tagtattttg taaaggagtg tgattatatg aaaaaattaa ttcatttttt
     2581 aaaaccatat agagttttaa ttgttgtagt tttgatattt acgtttttac aaacacttgg
     2641 aactttgtat ataccaacac ttacagcaaa tatagtaaac aatggtgtag taaagggaga
     2701 tatagattat attgtaaaaa caggtttaat aatgatgata gtagctggta taacagcttt
     2761 atcagctgtg ttagtttgta aagtatcagc aaatctttca tctggatttt gtagagatat
     2821 tagagaagca gtatttgtaa aatcacaaga tttatcaata aatgatttta ataatatagg
     2881 tacagcatca atgattacta ggagtactag tgatattact ttaataggtc agtctgtgtt
     2941 tatgtttatt caattggtat taccagcacc aattataact gtttctggtc tattcttagc
     3001 atattctata gataaagcaa tgacaattat tattgttgta gtaatgtttt tattcatgct
     3061 ttcagcattt ttagttggta aaaagcttat aaagttgttt aaaatgatgc aaataaagat
     3121 ggacaatatg aatagggtat tacgtgaggt agtaactgga gtaagagtta taagagcttt
     3181 taatagggag cattttgaaa aaaagagatt tgatagaact gcaatagatt attctgaaac
     3241 agcaatttca ataaataaag tatttgcagt acttatgcct attgtaatgt taataatgaa
     3301 tttaggtata gtaagtatta tttggtttgg tgggatgaga gtcagtaatg gaaatatgga
     3361 gataggtcat ataatggcac ttattgaata ttgtatctta attttattct atttaatcat
     3421 gggagtaatg gtatttatgt acatacctag agcaggtgca tgtgcagata gagtaaatca
     3481 gatattagat atagaacctg aaattgtaga tggaaatggt cgtaaagata ctgtctcaga
     3541 gaggggacat ttagtattta aaaatgtaac attttcatat gcacaatcag aagaacctgt
     3601 gttaaataat ataacatttg aagcaaaatc tggagaagta actgcaatta taggaagcac
     3661 tggctcaggc aaatctacta ttgcaaatat aataccacgt ttttttgaaa ttcaatctgg
     3721 agaaatatct ataaatggtc aggatgttaa gaagattcct caaaaagaac ttagagataa
     3781 gattggattt gtgccacaaa aagctttttt atttagtgga acaatagaag aaaatataag
     3841 gtatggaaaa gaagatgcaa gtattgaaga aataaagcat gctgcaagta tagctcaagc
     3901 tgatgaattt atttctgata tggaagacaa gtatgactca tttgtagcac aaggtggaaa
     3961 taatttgtca ggtggtcaaa aacagagaat atccattgca agagcgttag ttagaaagcc
     4021 tgaagtatat gtctttgatg atagtttttc tgcattagat tttaaaacag ataaaagact
     4081 tagaaaagcg ttgaaaaatg aaatcaaaga ttcatcagct ataattatag cacaacgtat
     4141 aagtacaatc atggatgcta atcagattat agttttaaat gatggaaaaa ttgtaggtat
     4201 tggaaaacat aaagatttac ttgaaaattg tgaagtttat aagcaaattg cggattcaca
     4261 acttagtaaa gaagaacttg cataaaatga agaattaatc tgatatttgg aggtgtatga
     4321 tatgagtgaa aaaagaatag atgaacaaga tatagaagta cttagttttg aagatggcat
     4381 gtcagcagaa aaagttaagg atgcaaaaaa aactactaag agattattaa aatatatggc
     4441 aaaacaaaag tttaaaatat taattatttt tatttcagta ttgataagtt cagcgcttac
     4501 agttttagca cctatggtta tgggaaaagc tattgaccaa ctatttaatg gaataaaaac
     4561 tgcagtccaa acaggtacaa aattcagtgt gaattttagt actatggggg ggatagtatc
     4621 aatacttcta gggttgtact taattagtgc agtatttata tatattcaac agtatattat
     4681 gtcaggagtt gcacaaaatc tagttcttag catgagaaaa gatttaagtg ataaactcaa
     4741 taaacttcct cttaagtact atgattctca taaaaagggg gaaacactta gtatagtaac
     4801 aaatgattta gaaaaagtag cagatagttt acaagaagga ttaatgcaac ttattacagc
     4861 agtagttaca gtagttggtt ctattgtaat gatgataagt ataagtgtac cacttacaat
     4921 agtatcagct ataacattac ttgtttcatt gggtattact gttgtaattg caagaaaatc
     4981 acaagaaaga ttctctgaaa atcaaaaggc actaggagag ttaaatagca atatagaaga
     5041 gatatttact ggtcaaatag tggttaaagc atttagtaaa gaaaaggata ctataagaaa
     5101 ctttaaaata ttaaatcaaa atctttataa tgcaagtaga agagcacagt tttcgtcata
     5161 tgcaatatct ccaattatta gatttataaa cctaattggc tatgttatta tagcagttgt
     5221 aggtgggata tttgcatcaa ctggagcaat gactcttggt agtatacaag cgtttattca
     5281 atatgtaaat caagcatcag agcctactac agaaatatct tatattgtaa atatgcttca
     5341 agcagcaata gcatctgcag agagagtttt tacagttatg gatgaggtgg aagagataaa
     5401 ggatgaagaa tcttcaaagg ttattagcat gcctaaaggt aaagtacaat ttgagcatgt
     5461 taaatttgga tatagtgatg attttattct tatgaaagat attaatatca atctaaatgc
     5521 aggagacaag attgcaattg ttggacctac aggagctgga aagacaactc ttgtaaattt
     5581 acttatgaga ttttatgaga tacaaggtgg aagaattact attgatggtg ttaatataaa
     5641 agacttaaag cgtggagaac ttagaactat gtttggtatg gttcttcaag atacatggct
     5701 gtttaatgga agtataaaag aaaatattgc atatagtaag tcagatgcca caatggatga
     5761 aattgtaaga gcagcaaaat cagcaagagt agaccatttt ataaggactt taccacaagg
     5821 gtatgacact atattgactg aggatgcttc taatctatct caaggtcaaa agcaacttct
     5881 aacaattgcc agggcaatac tttcagaccc atcagtattg attcttgatg aagcaacatc
     5941 tagtgtagat actagaacag aggtagaaat tcaaaaggca atgaataact tgatgaaggg
     6001 aagaactagt tttgttattg cacatagatt atcaactatt cgagatgctg atttaatatt
     6061 agtaatgaaa gaaggcacta ttattgaaca aggaagccat aaggaactta ttgaaaagaa
     6121 aggattctat gaagaacttt ataatagtca attttcaagt gaatatgatg aagaagttgt
     6181 atgattatca agttttaggc aactaaatga ttgataaata ttaagttaaa tatagtaaca
     6241 tgtaaggtta tatggaataa gagtatttct ctatttcata taaccttttt tatttatcaa
     6301 aataagataa aattttcttt ctaaattaga ttgaagaaag tatttatcaa tataaaagta
     6361 aaataatcat tattattagt gaagtaaata ttgtttgcca catatatgcc ataaagatgc
     6421 acttatttta cggagattta acaaagtagt gtaaagtata ctctaaatat aaaataaatc
     6481 aaatagagtt aaataaattg agaggtgata taaatagtga ataaaaaatt gaactataga
     6541 tttgaaatga agcacaaaat aactgaagca gatgtgttag ctttaaaatc aagactctat
     6601 ccaataatga agaaagatga aaatgcatct aaagatggaa aatatcttat taggagttta
     6661 tattttgata ctcctgaaga taaagcactt ttagataaat taaatggcgt tgcaataaga
     6721 gaaaagttta ggataagatt ttataataat aactgctctt atataaggtt agagaaaaaa
     6781 atcaaacatt acaacatgac atcaaagcta tcaactaatt taaccaaaaa agaagtaata
     6841 aatatactaa acaatgatat tgaatttcta aaagagtcga caaattgttt attaagagaa
     6901 ttttatttga aattaaaatg tgaaagatta gaggcaaaaa gcatagttga ttacaataga
     6961 gaagctttta tttatccagt tggaaatgta agagttacaa tcgatagtga tattaaaact
     7021 tctgtaaatt cgatagattt atttaataaa gatttgccga ctgtttcggt aattgatgag
     7081 aatatgactg tattggaggt taaatatgat gaatttatac cagattttat aaaggattta
     7141 attcaaatta ataagactac ttctacagca gtttcaaagt atgcttcaag tagattatat
     7201 atttaagaat aaaggctatg atttatataa agatatacat aaataaggtt aaaaaacaaa
     7261 taggttaaaa ataaataaaa taaaaaataa ataagttaaa aataggaagg atgattttaa
     7321 ttatgacatt taatgatatt tttaaatcaa gttttataga aaacgtatca ggtttttcat
     7381 ttgtggacag tgctttagca ctaggttcag cttttttagt aggtttgttt atatatatgg
     7441 tctataaaaa gacttatatg ggaattatgt attctcgacc attcaatgtg tcgcttgtag
     7501 cacttacaat gcttacaact tttgtaatat tggcggttac ttcaaatgta gttttatcct
     7561 taggtatggt tggtgcttta tctatagtaa gatttagaac agcaattaaa gacccaatgg
     7621 atttagtatt tctattttgg tcattgggtt caggtatagt tttaggagca ggtttgattc
     7681 ccttagctgt catgggttca ataattatgg gattgattct aatattcttt tcaaataaaa
     7741 caatatctga aactccatat atattgatgg taaattgtaa taatgaagat tcagaagata
     7801 tagcaactga taaaattaaa aaagtattta ctaaatatca aataaaatca aaaagtgtta
     7861 caccagaaaa aggaatagaa ttagtgtttg aagtaagaat gaaatatgga gagacaagtc
     7921 ttataaatga cttgagtcaa gtagatggag ttactaatgc agttcttgta agctataatg
     7981 gtgactatgt agcatagaga ggagtaatct tttatgaaag ataaaaaatt tacccttctt
     8041 atctctatta tgattatatt tttatgtgct gtagttggag tttatagtac atctagcaac
     8101 aaaagtgttg atttatatag tgatgtatat attgaaaaat attttaacag agacaaggtt
     8161 atggaagtta atatagagat agatgaaagt gacttgaagg atatgaatga aaatgctata
     8221 aaagaagaat ttaaggttgc aaaagtaact gtagatggag atacatatgg aaacgtaggt
     8281 ataagaacta aaggaaattc aagtcttaca tctgtagcaa atagtgatag tgatagatac
     8341 agctataaga ttaattttga taagtataat actagtcaaa gtatggaagg gcttactcaa
     8401 ttaaatctta ataactgtta ctctgaccca tcttatatga gagagttttt aacatatagt
     8461 atttgcgagg aaatgggatt agcgactcca gaatttgcat atgctaaagt ctctataaat
     8521 ggcgaatatc atggtttgta tttggcagta gaaggattaa aagagtctta tcttgaaaat
     8581 aattttggta atgtaactgg agacttatat aagtcagatg aaggaagctc gttgcaatat
     8641 aaaggagatg acccagaaag ttactcaaac ttaatcgttg aaagtgataa aaagacagct
     8701 gattggtcta aaattacaaa actattaaaa tctttggata caggtgaaga tattgaaaaa
     8761 tatcttgatg tagattctgt ccttaaaaat atagcaataa atacagcttt attaaacctt
     8821 gatagctatc aaggcagttt tgcccataac tattatttat atgagcaaga tggagtattt
     8881 tctatgttac catgggattt taatatgtca tttggtggat ttagtggttt tggtggaggt
     8941 agtcaatcta tagcaattga tgaacctacg acaggtaatt tagaagacag acctctcata
     9001 tcctcgttat taaaaaatga gacacacaaa acaaaatacc ataaatatct ggaagagata
     9061 gtaacaaaat acctagattc agactattta gagaatatga caacaaaatt gcatgacatg
     9121 atagcatcat atgtaaaaga agacccaaca gcattttata cttatgaaga atttgaaaaa
     9181 aatataacat cttcaattga agattctagt gataataagg gatttggtaa taaagggttt
     9241 gacaacaata actctaataa cagtgattct aataataatt ctaatagtga aaataagcgc
     9301 tctggaaatc aaagtgataa aaaagaagtt aatgctgaat taacatcaag cgtagtcaaa
     9361 actaatacag ataatgaaac tgaaaataaa actacaaatg atagcgaaag taagaataat
     9421 acagataaag ataaaagtgg aaatgataat aatcaaaagc tagaaggtcc taggggtaaa
     9481 ggaggtaagt caataccagg ggttttggaa gttgcagaag atatgagtaa aactataaaa
     9541 tctcaattaa gtggagaaac ttcttcgaca aagcaaaact ctggtgatga aagttcaagt
     9601 ggaattaaag gtagtgaaaa gtttgatgag gatatgagtg gtatgccaga accacctgag
     9661 ggaatggatg gtaaaatgcc accaggaatg ggtaatatgg ataagggaga tatgaatggt
     9721 aaaaatggca atatgaatat ggatagaaat caagataatc caagagaagc tggaggtttt
     9781 ggcaatagag gaggaggctc tgtgagtaaa acaacaacat acttcaaatt aattttaggt
     9841 ggagcttcaa tgataataat gtcgattatg ttagtaggtg tatcaagggt aaagagaaga
     9901 agatttataa agtcaaaata agattaaaat tgaatataaa taattttaat actgttaagt
     9961 ctaaatattt acttagcagt atttttttag aaaataatgt gtttgattaa taatataagg
    10021 taataaattg ataaaaatta aatacagcat attaataata cataataaag taataaaaaa
    10081 actgtttaag tataataact tatatttaga cagttttttt catttataaa taatataaat
    10141 gaaattgaaa acatttgaag gatagaaatt ggttggataa atgtataaat tacttcacca
    10201 aaaaataatg acaagagttg taactaagtt gtaataaaat ggtagttatt tagcataaaa
    10261 taatgtatca tagaagtata ttataaaatt aataaaataa gatttttgta agaaaactgc
    10321 tggcttttga tatttagagt gagtagttaa attatgttta ggaggggtag aatttgttgc
    10381 aaaaagtatt atggaaattt ttaaagctag tagtcggttt atttatatgt tcagttggta
    10441 tagtattgac gataaattgt aaccttggat tatctccctg ggatgtattt catcaaggac
    10501 tttcaaatca tataggaata actataggaa ctgcttctat aattgtaggc tctatagtag
    10561 ttattgctga tgtcgttctt ggagaaaatg taggatgggg aactgttttt aacatgctac
    10621 taattggatt ctttatggat ttacttttat atagtaattt aattccagag gctgatagtt
    10681 tatttgtagg aataattatg ttgatattag gattggttct tttgtctgtt ggaatggtat
    10741 tttatatggg aagtggttta ggaagtggac ctagagatgg tttgatggtt gcaattcaaa
    10801 agaaaacagg aaaatcactt aaattaataa gaggtactat agaagttgga gcattaatag
    10861 taggattttt attaggtgga aaagttggta taggaactat aataagtgca tttggattgg
    10921 ggtatttcac acaaatggta tttggcttat ttaaattaga ttgtagtaag attaaacata
    10981 gatttatagt agatgatata aaatttatca aagtgtatat gagtggtgag aaaaaagctg
    11041 ctgttcaaaa tatagttaag taatttaaga ataaattcaa cgaaatgtta agtattttaa
    11101 attaacattt tatttagatg gataaattga atattaaaag aatattaaaa tatgaggatt
    11161 gctagaacac tataaaatat agatttctag caatttttta tttgttcaat gtttttttag
    11221 tactagaagt cttattctct atttaatgaa tgaattaatt tataattaga gatactaaac
    11281 tttatagata ttaatataaa cacctttaaa ctgtgtattt tccaaagcct ttaaaatgct
    11341 tgtatttatt ctaaataaaa aaataaaaaa atgtcaaaaa aactcttgta ttatatccaa
    11401 attttatata taattaccta tgttgtttag aaattttaaa ctcaatgttt agtaaacaaa
    11461 atgtttagta aaataaaaca gaaactagga ggtgcaacat ggatataggt gaaaagataa
    11521 aaagattaag aacagaaaaa caactgaccc aggaagagct agctaataga tgtgaattgt
    11581 caaagggatt tatttctcaa ttagaaaata acttaacatc accatccata gctaccctta
    11641 tagatatact tgaaatacta ggtacaaatt taagggaatt ttttaatgaa atagatgatg
    11701 aaagaatcag ttttacaaaa gaagatatgt ttgaaactga agatgaagat ttaaaatata
    11761 aattaaagtg gttaattcca aattcacaaa aaaatgaaat ggagccaata ataataacat
    11821 tgtatccagg aggacagtat aaagaagaaa agccacatga aggtgaagaa tttggatatg
    11881 tattagcagg ttcaatatat gtccatatag gcgaaaagaa aaataaagta aaaaaaggag
    11941 agagttttta ctttagacca aaagcaaatc actatatatc aaacgaaggc aaaactacag
    12001 caaaagtgat ttgggtaagt acgccaccat cattttagaa tagaggtgtt taataattgg
    12061 tagaaaatat aatagaatta agggatatat ctaaacatta tgaggaactt actgttttag
    12121 ataatttcaa tttagatata aagaaaaatg agtttttaac attacttgga ccaagtggat
    12181 gtggaaaaac tacaacatta aaaataatag caggatttga gtatgctgat gatggtaaag
    12241 ttttatttga aggtaaggag ataaataact tgccaccata tgaaagacaa gtaaacacag
    12301 tatttcaaaa atatgcttta tttccacaca tggatgttta tgaaaatgta gcttttggat
    12361 taaaaattaa aaagactcca aaagatgtaa tagatgctaa agtaaaagag gttttgaaac
    12421 ttgttgcact agaaggtttt gaaagacgac aagttgaatc attgagtgga ggacaacaac
    12481 agagaattgc aatagcaaga gctctagtca atgaaccaaa ggtattatta ctagatgaac
    12541 cacttggagc acttgattta aagttaagac aagagatgca aatagaatta aagagaatcc
    12601 aaaaaaaatt aggaataaca tttgtttttg taactcatga ccaagaagag gctcttacta
    12661 tgtcagatac tattgttgta atgtataagg gcaaaattca acaaatggga acaccacaag
    12721 atatatacaa tgaacctaaa aattcatttg tagctaaatt tataggtgaa agtaacatat
    12781 ttgatggaat tatgatagaa gattataaag ttaatttctg taatagagat tttgagtgtg
    12841 ttgataaagg attcgaaaaa aatgaaaata tagaagtagt aataagacca gaagatataa
    12901 aaatggtaaa gccagaagaa ggaatgttaa aggggaaagt tacatctaca gtgtttaaag
    12961 gagtgcatta cgagatagaa ttgaatgaaa atggaagatt gtggatactt cataatacca
    13021 aaaatgctga ggttgggact gaactaggca tggatatata tccagaagat atccatataa
    13081 tgagaaaaga gaataactaa gatgaaaaga aaatcttttt tagcatatcc atatgtagtt
    13141 tggagtgcga tatttgtaat aattccttta atattagtgg tattttttag ttttacaaaa
    13201 gaatcaggtg gaggttatgc ctttacactt gagaattata aagaagtaat tgaccctata
    13261 tatatgaaag tttttgggcg ttctattctt ttagcaggag gagctacatt aatatgcctt
    13321 atagttggtt atccagtggc atatataatt tcaaaggctc gtgtaagtag aagaggttca
    13381 ttaatattat tgtttattct tcctatgtgg atgaactttc ttttaagaac atatgcatgg
    13441 gttgctatat taggaaaaaa tggactttta aatacatttt taggttggtt tggaatacaa
    13501 ccattagcaa ttttgtatac taattttgct atacttttag gtatggtgta taacttttta
    13561 ccatttatgg ttcttccaat atatacagca ctatcaaaga tggataatga tttgataaac
    13621 gctgctcatg acttgggtgc aaataatatg acagttttta gaaaaattat atttcctttg
    13681 agtttgccag gtgtaatgtc tggaataact atggtattta tgccagcagt cactactttt
    13741 gcaatatcaa gactgttagg tggaggtaaa ataatgctgg ttggagattt gattgaacaa
    13801 cagtttacag ttgttggaga ttggaacttt ggttcagcta tatcgatatt tatgatgata
    13861 gtaatattaa tatctatgtc tataatgtca aaatttggag atgaatctga caaagaaggc
    13921 ggtgggttac tattttaaaa ttaaagaaaa taacttctaa tatatattta gcattagtat
    13981 tcttattttt gtatgcaccc atatttgctt tggtactatt ttctttcaat gattcaaaat
    14041 caatggcaag atggaatgga tttacatgga agtggtatgg tcaattactt caaaatgaga
    14101 gtataatgag tgctttatat tatactattg taatagctat attagcgtct gtaatatcta
    14161 caatagtagg aactataagt gctataggaa tacataagat gagaggtaag agtaagaaat
    14221 taatactaaa tgtaaactat cttcctattt taaatacaga gatagttaca gcagtagctc
    14281 ttatgagcct gtttgtattt gtaaaaatgg aatttggatt tacaacaatg ctattggcac
    14341 atataatgtt ttgtttgcca tacgttatac tttcagtttt gccaaaaatg aagcaattac
    14401 ctgataatat agaagatgca gctatggact tgggagcaac acctatatac gctcttagaa
    14461 aagtaatttt accacaaata aaaccaggta tagtttctgg atttttaatt gcatttacta
    14521 tgtcaataga tgactttata ataagtttct ttaatgctgg aaatggtgta agcaatcttt
    14581 ctatagaaat ctatggaatg gctagaagag gtataaaacc tgaaataaat gcattatcaa
    14641 caataatgtt tgcagtggtt ctaggtttac ttttactagc taataaaaaa gaatctatag
    14701 taagggggat taaataatga ttaagtttaa aaaaacaata tcccttatag ctgtaggtat
    14761 gatgacagtt actttattta caggatgtaa taaagcaact gattctacaa aagttttaaa
    14821 tgtatataat gttggagatt atatagatga gtctcttata gataagtttg aaaaagaaac
    14881 aggaatagat gttcaatatt caacttatga tactaatgaa atgatgtatc aaaaagtaaa
    14941 gagtggtagt acacactatg atttagtatt tccttcagac tatatggtag agaaaatgaa
    15001 aaatgaaggt cttttggaaa aattagattt taagaatata cctaatatga aatatttaga
    15061 taagagtttt ttaaatcctg tatacgacaa aactaatgaa tatagtgttg catatatgtg
    15121 gggaactctt ggaatattgt acaataagaa agaagttaaa gaccctatgg atagctggaa
    15181 tatactttgg aatccaaagt ataagggaaa tataatgatg tttgactctg ttagagatac
    15241 tatagggata actcttaaaa aattaggata tagcatgaat agtgtaaatc caaaagaaat
    15301 aaatgaagct aaaaatcttt taatgaaaca aaaggattta gtattggctt atgtaaatga
    15361 tgaaggtaag gatagattgc ttggtggaga agtggcaatg ggtatgctgt attctggaga
    15421 tgcagttacg ctaatggagc aaaatcctga ccttgattat gctataccta aagagggaac
    15481 taataaatgg gtagatgcta tgtgtgtacc tacaacagca caaaataaga aagaagctga
    15541 gctgtttata aactttttat tagacccaga gaatgcaaaa gtaaatgctg agtacattgg
    15601 gtattcaaca cctaatacag gagcactaaa attattagac ccagaaataa ctcagaatcc
    15661 tgttgcatat ccatctaaag aagtacttga taaatgtgaa acttttgaag atattggtga
    15721 aaatataaaa ttatatgaca aggcttggat agagttaaaa tcaaaatagg gtcttgttgg
    15781 tttaagtaaa tttacttaat aaatacttaa taaaactgtt cttttttaga aaatttattt
    15841 tctaattatt gaacagtttt attttttgtt taggtcatgt ttttattttg agtttatttt
    15901 tatgagaatt taaagtacag taagtttcat tgattactat ttttataata ataactataa
    15961 agaggttgtc tcgaaattga gataagcctc tttttcattg ttgaaatgat gaaaacatgt
    16021 ttgatttcat aaaaaaagag tgattcatga gctaaagaat ttgctttgag acagtatttt
    16081 ttattaagtt atattcatat gcaatttaaa tagtttatta ataaatgaaa acttatttgt
    16141 ataatgattt taataataat tagaaaaaat gggcaactta atagaataat tggaggtgta
    16201 aatttataaa aaatgaatag gataaataaa aaaatagatt tattaatatt ggctttgctc
    16261 gtttttatat ttttaattgt tggaatagta atactatcga ttaggacaga aaataaattg
    16321 aatgtgttca taatgttagc aataattttt tttataataa tgttaactta tttatcaaat
    16381 tcagtagtgg ggttgataac aagttcaata attatattta tgtatacatc ctatatttta
    16441 tataacaata ttactcacaa tatggatgtt gaatttatat catatatgtg gataatagca
    16501 acaccagtat cttcaattat aatgggtaat ctgaacaaaa gcataaatga attgcaaaat
    16561 acaaataaaa aactatctga acagtataag gaattagtga ctattgatag tgaaactgga
    16621 cttagaaatt taaagatttt ttataatgat gtaaatatgg agataagtaa atctattaga
    16681 cataatactg atttttctct tatgatagta aaattaccat actacggaaa cctacagact
    16741 atatttggtg aaaacaaaac taataaaata gtaaaatata ttggttcaaa tattatagaa
    16801 tgtacaagaa atgaagatat aatttatagt ttacaaaaag atatgatagg gatactaatg
    16861 ccaaatacat ctttggaagg ttcgaaagtt gttaaagaca gaataaagaa aaggataaaa
    16921 gaattaaatt tagatttaaa caatagggga aaatatgtaa atatagatgt caaaattgca
    16981 tttttacaat ataagagtag ttttggagat agtataaatt ttaagaatat agttgaagag
    17041 gaacttcaat atgatgttta aaatatttaa taaaagttta ttaaaattag taattgtatt
    17101 atcaatagta ctgacaatat caaattttag cttattttca tatgcatcag aaattacttc
    17161 cagcacagat aaggtattaa tcatatatga ttctaagaaa gaaacagctt ataatagaga
    17221 tattttaaat attatgagaa ctctattggg aagatttagt tcagatatag agctattaaa
    17281 attgagtaac tatgatggtg agataaatga aaattattac agtcatatat ttattttagg
    17341 aatcaatgaa aactcatata ataatgataa aaacactaaa aacttgatta gttccttaaa
    17401 ttcatataaa gtaactatat gttggttagg atatggtata gaaaatcttt tggaacataa
    17461 aaaatacaat ttagactatg taggaaaaac aaacaatata gttagtgtaa attatagagg
    17521 taaatcatat aacttggatg aacattatgt ttttaacatt gtagaatcaa aagatacaag
    17581 taataaagta ataggaagta ttaatgatac cttaaataag tatccatata taataaatga
    17641 taaaaatctt ttttatgtat caaaattaga tttagatgga gttttatttt atatattttg
    17701 cgattcactt aatgatattt ttaatattaa gacgtttgat aaaggacgta tatttgtaag
    17761 aatagaagat gtacatgctt ttagagaacc aaaaaatctt gtagagatag cggattattt
    17821 aagtagtaag aatataccat ttacgattgc attaatacca gcatatgtaa atcctaaaaa
    17881 tcataaagta ataactttat cagaaagccc tgaaattgtc aaagcaataa aatatatgca
    17941 agataaaggt ggaactgtga tattgcatgg atatactcat caatacaaga aagaagaagt
    18001 ttctggagaa gggtatgaat tttgggatgg taaaaaagat gaacccttaa aagaaaatat
    18061 gaagatattt gtcaaagata gagttttgaa tggtcttaga gtatgtatag agaatggaat
    18121 ttatcctctt gcatttgaag cgccacatta tgcaatggaa tcagagggat ataaagaatt
    18181 aaaaaaatat ttttctactt atatggggca acatcaaaat aatgataaaa aatttagcac
    18241 aaatacatat ccttatatta ttagggatac agaggagttt aatatattta tacccgaaaa
    18301 tttaggatat atagacccag aagacaagtt tacttttcaa aatataaaag aaaatttaga
    18361 taaattatct attgtaagag gttttagtgg agggttcttc tttcattcat atttaaacat
    18421 agagtatcta aaaaacacaa tagaatattt agaaaaacag aatatagaat ttatgaattt
    18481 aagagatttt aataattggg taaaagttga tgaaatacaa ataagaaata atggagatga
    18541 aattatagtc aactatgata aggacttaga tgaaataact aaaagtgata ctagatttaa
    18601 atacatatcc aatattagta aaattttaat attcatagta tctataagtg ttttaatatt
    18661 tgtgat
//
LOCUS       DAMJVX010000067        18557 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.070, whole genome shotgun sequence.
ACCESSION   DAMJVX010000067 DAMJVX010000000
VERSION     DAMJVX010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18557
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.070"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(58..471)
                     /locus_tag="PC407_002024"
     CDS             complement(58..471)
                     /locus_tag="PC407_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2497470.1"
                     /translation="MILVSACLIGINCKYSGDNNENEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKIKSEKDL"
     gene            complement(539..2917)
                     /locus_tag="PC407_002025"
     CDS             complement(539..2917)
                     /locus_tag="PC407_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2497471.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSEISAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRNLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            3234..4685
                     /locus_tag="PC407_002026"
     CDS             3234..4685
                     /locus_tag="PC407_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDF2497472.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(4889..7402)
                     /locus_tag="PC407_002027"
     CDS             complement(4889..7402)
                     /locus_tag="PC407_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2497473.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTSDEQLEVLKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTDLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKDTEKLKVGVLLNKPPFQNADKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLEDMAKKHEIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLNGK
                     RIALAKGIDYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYEAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKSDKESENGNIENERDKIRREYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRITSKIIYNDRKIPMYVIGKIANIDSEKEEKNRLLDKVQR
                     DSLTNIYNAAYIRKLTTQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLVGDKVLIDV
                     AKVLDESFAKDDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVITKRGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(7625..8446)
                     /locus_tag="PC407_002028"
     CDS             complement(7625..8446)
                     /locus_tag="PC407_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2497474.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMIVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEMR"
     gene            complement(8965..9441)
                     /locus_tag="PC407_002029"
     CDS             complement(8965..9441)
                     /locus_tag="PC407_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HDF2497475.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVGNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(9475..10053)
                     /locus_tag="PC407_002030"
     CDS             complement(9475..10053)
                     /locus_tag="PC407_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2497476.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(10157..12775)
                     /locus_tag="PC407_002031"
     CDS             complement(10157..12775)
                     /locus_tag="PC407_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2497477.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIETGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MNRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMASSISIDSSISIDSSISIDSSISMDLDTSSSLGIPKELMGSNTNNIDTLSNTSK
                     IDTLNKNHYNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFK
                     KKDLNIDCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGY
                     APMMVTEYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSK
                     VTCMLDNLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKK
                     LDEIGTVAGHYYKGVE"
     gene            complement(13149..13832)
                     /locus_tag="PC407_002032"
     CDS             complement(13149..13832)
                     /locus_tag="PC407_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDF2497478.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(14174..14764)
                     /gene="zapA"
                     /locus_tag="PC407_002033"
     CDS             complement(14174..14764)
                     /gene="zapA"
                     /locus_tag="PC407_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDF2497479.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDVYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(14789..17182)
                     /locus_tag="PC407_002034"
     CDS             complement(14789..17182)
                     /locus_tag="PC407_002034"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDF2497480.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(17199..18218)
                     /gene="pheS"
                     /locus_tag="PC407_002035"
     CDS             complement(17199..18218)
                     /gene="pheS"
                     /locus_tag="PC407_002035"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDF2497481.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(18268..18502)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         5711 a         3443 c         2042 g         7361 t
ORIGIN      
        1 ctatttttat acatcaatat tagctttata tacttttttt actcttttat ctattttcta
       61 taaatctttt tcactcttaa tttttattcc ttcttcttct aacagccttg ctgttatacc
      121 tttccctggt atctttttac ctgaaaaact accatcatat attagattac atccacaaga
      181 ggggctaccc tcttttagta ttgcttcgct acatacatat aaatttgcta tttttagagt
      241 ttcattagcc cctcttacaa agttttctgt tacatcctta ttatctttag taattacttt
      301 tccatcaact atctcagcag gtggtcttgg tgtagataga ccacctaact gctctgggca
      361 tacaagtgta aattctttgc cttttaaata ttccttgact ttttcatttt cattattatc
      421 gccactatat ttacaattta ttcctattaa acatgcagat accaatatca taaaaatgtg
      481 ctcctataca aattcttaat atattcttat acaaattact aatgtattct tatacaaatt
      541 attttaattt aacaattgtt actcctgctc caccttctcc atattgacca cctcgttgtg
      601 attttacatg tctatttctt cttaaaatgt cttgtagccc tgcttttaat actcctgtgc
      661 ctattccatg tattactgtt acagactcta aacctgccac atatgcatca tcaagatact
      721 tttctacttc cattatagct tcttctaggt ttaaccctct aaggtcaact tcatttttta
      781 cactaccaga ttttgacctt ataatgtttc tcgttgattt agttacattt gttgaaacat
      841 ctttgcgtgt tttttgtaaa gatttaaaag gtagagtcat cttcattata ccaatttgca
      901 ctactgcttc ttttctcttt ttatctactg aaactacact accattttga tttaaagtta
      961 ttaccttaac ttcttcacct ggctttaagt cttttatctc cttatttgat acctttggaa
     1021 ctatcatact ttttactgtt ggctgaagac ttcccataga gctggtcaat tcttttctaa
     1081 gttcttctat cttacgattc ttttcttttg aagctctttc ttgttcaaga tttctaagtt
     1141 cttttattat aatatctact tcttctttag cttgtcttat aattgaaaat gcttctgatt
     1201 tagctttttc aatcatctta tctctttgac tgaccaattt ttcaagtttt tcgtcatact
     1261 ctactttcag tttttcaatc tcttctttta atctttcagc ttcttctcta tcttctactg
     1321 ctttgattct attcttttca acattctgta acacatcttc taaagctatg ttttctgtat
     1381 ttatatattc tttagcacgt gaaatcacat aatcacttag cccaagtttt ctagaaatct
     1441 caaacgcatt tgattttccg ggaacaccta taagtaactt atatgttggg cttaaagttt
     1501 ctatatcaaa ttcaactgct gcattttcta cacctggttt agtaagagca taatttttaa
     1561 gctcactata gtgagtggta gctatacatt tagctcctac agaatttata tcttctaata
     1621 ctgctattgc aagggcagct ccttcaacag ggtctgttcc tgctcctaac tcgtcaaata
     1681 taactaaaga gtctgctgtt acgttttgta atatagatac tatattcgtc atatgagatg
     1741 aaaatgttga tagactctgt tcaatactct gttcatctcc tatatctgca aatacattat
     1801 catatacaca catactactt ccataatcag caggtatatg aagtccactt tgtgtcataa
     1861 gtgcaaatag acctacagtt tttattgtaa cagtcttacc acctgtgttt ggacctgtta
     1921 tcaccaatgt atggaagtct cttcctaaat aaattgtatt tgctactacc ttctttttat
     1981 ccagcagtgg atgtcttcca ttttttatgt ttaaatattt atcctcattt aaagttggtt
     2041 ctattcctct catctgtata gaaagttttc cctttgaaaa tgcaaagtcc aatcttccaa
     2101 gtatctcttg attagagatt aaatcttcgc tcacttctcc aaccatagct gaaatctctg
     2161 ataaaattct ttctatttcc tcttgctctc ctagtttcaa ctgcctcaat tcattgttca
     2221 tctctactat agtcattggc tctataaaaa gtgtcgcacc agaagatgac tggtcatgca
     2281 ctataccagc tacttgcgac ctatattcag attttacagg tactacaaat ctatcacctc
     2341 ttagagaaat tattgcatct tgtaagtact tttgataagt agttgaactt attattgaat
     2401 ttaattttga cctaatggat tgattttttt gggctattct tcttctgata tctctaagtg
     2461 tacttgaagc attgtcagat atttctactt cactaattat agcattgtat atctggtctt
     2521 ctatgtcctt gtacacatat agagaatttg atagagattg tattattgga taattaaaat
     2581 cttcttcatc actactagat aagctatttc ttaatcttct agccactctt aaggtatctg
     2641 ctatcataat cagacttcct gggtcaagag aagcacctat ataagctctc tttaccttat
     2701 cttctatatc atgaatacct tccaacccta ctgaacctct ctttataata attgattgag
     2761 cttcactagt ttcttctagc atagatttca cttctacaaa atcagtattt ggtactaaat
     2821 tttctatata ttttagacct aaagaagatg aggctttctt ctttaacaaa tctattattt
     2881 tattgtattc tagaactctt aacgatttct cattcatttt ctatattcac catcctcgat
     2941 attttaactt ttttatttaa ttttaaaaat gtatgttttc aagcatttcc aataaaaaat
     3001 gcaagtttta tttctttttc atttctaaaa ttattatatt ttttctcttt tagatatatt
     3061 ttacacatat tttgtattat actatgtatg tagctatcta tatattatac ataaacatgc
     3121 tttatttgta atataatctg ttaaaaaatc ttctttaata tttttaaagg agtttatttt
     3181 agcttataaa aaaatagcta cttatataga taaattaagg aggaacatga gtcatgggaa
     3241 acgtattaga aggattaaaa ccagaaagtg tttttaaaaa ctttgagaaa atatctcaaa
     3301 ttcctagagg ttctggaaat gaaaaaggca taagtgattt tttacttagc tttggtaaaa
     3361 atttaggtct tgaaactatt caggatgaaa gcttaaacat aataataaga aagcctgcta
     3421 caaaaggata tgaaaattgt ccaggtgtag ttcttcaagg acatatggat atggtttgcg
     3481 aaaaagaaaa gaatgttgaa catgattttc ttaaagaccc tataaaatta agaattgatg
     3541 gtgacatgat atatgctact ggaactactc taggagctga caatggtata gctgttgcta
     3601 tgggtatggc tatattagaa gataatactt tagagcatcc tgcactagaa gttttagtta
     3661 ctgttaatga agaagatggt atgaatggtg cagatgcact agaccctagc ttaattaaag
     3721 gtcaatatat cctaaatatg gactccgagg aggaaggata cttattagtt agctgtgctg
     3781 gtggtaaaac ttgtgttgtt agtttacctg ttgaatacaa ggaagttaaa ggtgacaaac
     3841 aaggattatt agttgaggtt actggactat taggtggaca ctctggtatg gaaattgttt
     3901 tacaaagagc aaatgctaac aaagcaatag ctagagtatt aagtgtattg aatgttgatt
     3961 atgaattagc ttctgttgat ggtggtacta agcacaatgc tatacctcgt gaagctaaat
     4021 gtgtaatagc tgttaataaa gctgatgttg aatctgctaa aaaacaaata aatgacattt
     4081 taactgcatt taaacatgag tttactactt ctgaccctgg catgacttac tcagtagctg
     4141 aaacttctgt agacaaagtt cttactaaag attgtaaaga aaaagttgtt caaatgtctt
     4201 gcttaactcc tcatggagtt caatcagtaa gtcttgatat agaaggatta gttgaaagtt
     4261 ctactaactt cgctataatt gaaactaaag aatctactat tgaattttta acttctgtta
     4321 gaagttctgt tatgtctatt agagatgaaa tcgctgatag aataagactt ttggctcaag
     4381 ctttaggtgc taattacgac cttatagctc aatacccagc ttgggaattt aaaaaaggct
     4441 caaaattaga aaaaatatgc agtgaaactt atgagaaatt aactggtaaa gttcctactg
     4501 ttatggctct acatgctggt cttgagtgtg gattattact tgataaactt cctcatgctg
     4561 aagctatctc tataggacca gatatgtttg atgttcatac tccaaatgaa catgttagta
     4621 tcccttctgt agctaatgtt tgggattatg ttattgaaat attaaaatct atgaaccaat
     4681 attaaattta agttttgatt ctaaatagat tttaatttta agtaatctat ataaaattaa
     4741 ctctgtaaat taatactact gttagtataa tatttacttt caatattaac ttatagaaat
     4801 aaagcctaat gatagataat aacatgtatc attaggcttt attgtaattt aaatattatt
     4861 aaactttatt ctgttttaaa attaattctt atactatttt aaatctattt cttccttgtc
     4921 tcttagaatc ataaagagca ttatctgcta tttgatacag ttcatcatac tcttgaccac
     4981 gctttgttat aactaccccc atactaatag ataaactttc tccatttggc aacttaatga
     5041 gatgaatatg attgtataca tcattacatt tattgataag ttcttcctta tttattatat
     5101 catttatata aacactaaat tcatctccac ctaaacgacc tactatatca tcttttgcaa
     5161 aactttcatc aagtaccttt gcaacatcaa tcagtacttt atctcctacc aggtgaccat
     5221 aagtatcatt aattgactta aaataatcaa tatctataat caataaagct ccattttttt
     5281 cagttgataa atttaataaa ttttgtgtag ttaattttct aatatatgct gcattataaa
     5341 tatttgttaa actatccctt tgaactttat ccaacaatct attcttttcc tctttttcag
     5401 aatcaatatt agctatttta ccaattacat acattggtat ttttctatca ttataaatta
     5461 tttttgaagt aattctaatc caatgaaaag tatcatcaag catgatagaa tacatttcag
     5521 tagtcccatt ttcttgtgat tttattacat caaaaaactt tttattatat tctcttctta
     5581 ttttatctct ttcattttct atattgccat tttcactttc cttatcactt ttatccaaac
     5641 cataataagt ctcaataccc ttttctggca tagaaattat caatttatct tgcttataat
     5701 catactcaaa aaagtattca cttgtcattt catacaactg agtatgtttt ttgagttcca
     5761 aagatatctt ttcgttcata cttgtacgag tatgtaaacc aaatgccata aatccaataa
     5821 tacaaattaa taaacttgct acaataaaaa tagtctcaat tggattctgt tgcataatat
     5881 aaagcaacga gaaatcttgt ctatatgtag tattttggta gacaattgat tgcatttttt
     5941 ctgatgaaat acttataata gctttattaa atatattcaa taattcttgt ttatcttttt
     6001 taattatgcc aaaacaaata ctctttgctt cataagtttg aggaactagg cgcacatttt
     6061 tatacttagg ttgattcata tagtactgca tagaataacc ttctccataa gtgtaatctg
     6121 catagccttt attaacagct tctatacact cttctaaatt atcataccaa actatatttt
     6181 cttcaaactg accataataa tcaatacctt tggctagagc tatacgcttt ccatttagac
     6241 tattctcatt taatttatta ttaatcatca tagtatattg agtagaaaga aaaggccgag
     6301 acatagctat attatgttca cgagagtact cataatcata aatcattcct gctacaatat
     6361 caatttcatg ttttttagcc atatcttcca aatcttcttg agattttaca ggcacaagtt
     6421 caaattccaa acctgtttta tcacatatat gatttaacaa atcaattgat atacctctta
     6481 attctcctgt atctttatct gcattttgaa acggtggttt gtttaaaagt actcctactt
     6541 ttaatttttc tgtatcttta atgtaattag tttcttcatc agataaaaac atttgattat
     6601 tctctggcgt aaagtatttc tcatataaag tagttgaaaa atatgggtcc ttctgttcaa
     6661 tattattaat tgctgaatta agcttttgag ttaagtctgt actgcctttt ggagtaatga
     6721 aataaaatgg ctttgatgag aattttgcta cagtacgcac accttctatt gggttcatac
     6781 taacattaag aagtacatct gctttaccac tctttagtac ttctaattgc tcgtcacttg
     6841 ttttacaata taccaattca ggtgacataa gattcatttt acaatactca tctaattctt
     6901 taagcctagt atcagaattt tctattacag caatacgaat ttttttcaca tcttgcgaac
     6961 tcattatgtt atcaatatct gtattttcat ataatgcttg tagtactgta tttgctactc
     7021 cataactgtt tcccgcataa ttatatattt tttgcattgg ctcattgtat aacatacctc
     7081 ccattatatc aatttcacca ttttctaaca tcttcattaa ttttgtaata gattcattta
     7141 tctctccagg aacttgaaca aattcataat tccatccagt atactgtgca atttctagca
     7201 aatattcata tgtataccca aaatagttac cttgctcatc tatttcagtt aaaccttttt
     7261 gaataggata agcaacacga atggtacgat tatcattacc tatagcattg ataccaaaag
     7321 aaaatatgca agaagtgaat atagatgtaa ttacaataat taataaaagt aattttttta
     7381 aattcactac acaatcctcc atatagttaa gatttacagt ataaattact gcataaactt
     7441 gtagacatga aaaaatagat tagtatataa aaggatatat aaatctactt agaaatttaa
     7501 atatccttaa tatatacaat atatttaaat tataactcat ttatatatct attacaacaa
     7561 atttaccctg ctaaatacct actctatatc ccataatttt acaagttgta atgaagttta
     7621 agttctatct catttcaaat ttagcagtgt ttctatttac agttctttta tatttcacat
     7681 ctggataacc caatataaat gaagtgataa ctgcttcgtt ctcgctcatt cctaacagtt
     7741 cttttatctt tggctctaca tttgatgcca taacaaaaaa tccattataa caaattccta
     7801 atccttgaga ataggccata agttccatat tagatgcagc caatcctcca tctacataag
     7861 cacttccacc tagagacata tcacctacaa ctatcataag tagtggggca tcataaaata
     7921 aagaatcatt tccgttctct ttatatcttt tgtacatttt cttaaatgta tttttataga
     7981 tagaacgtac agggtcattg tcatcagtat ctaatgccaa attataaaga ccttgtattg
     8041 ccagttcttt cacttctttt agcttttctt ttactaatat gtatcttata ggctgtctat
     8101 taccacctgt tggtgtgtat ctaccagctt ccactatgtt ttttattttt tcttcttcta
     8161 cctctatatc tttatattgt cttatacttc ttctaaattt aataaaattc aataactttt
     8221 cgggttctaa ttcaaaactt tcacgattgt attctattac ttcattttta tcaaatcctt
     8281 gcatttctat tgcatctgtt gggcaaattg ccatacagtg accacagtta atacagagaa
     8341 tatttttaac ctttgcttta tcatccttaa cttttattgc atttacaaga cagtctgatt
     8401 cacacatccc acagcctaca catttttcta aatttatgtc tatcattcca aattattcct
     8461 ccttttaaaa aatttacttt agtataaaac aaatagttgt ctaagtcaat ttttattttt
     8521 ttaacaatat actagcctta actacacttt aatggagaaa aatatacata aaattagaac
     8581 ttaaattaga atttattcaa aataaaagat aaaagaagta aatgtctaaa agtgaacttt
     8641 ttagttaatg agacattctt tattgtatga aatcaagtgg atatttatca tttcaacaat
     8701 aaaaaaggct tacctctatt ttggatatgt ccttgttaag tagacagact aaaaaaggct
     8761 atctttatgc actgagatag ccccttttcc caactactat tgtttatttt aatattatat
     8821 caataaattt aatttgctat aacctgtaag ttacatttta atttgatgta actttaaatt
     8881 acatttttaa gtaatatctt taaattatat tcttaaaata tatcattaaa ctacatttta
     8941 attaatctaa aaactatatc ttaattaatc taagaaactt acattataat cttttagttt
     9001 cccattatca tatacaatct ttgcactaaa gatatttcca gtcttagccc aatttaagaa
     9061 caacctgtca ccttcccaca tatttaaatc gaatatttta tcattaccaa cccaaattaa
     9121 atttccttct ttacaatcat ttaaatcttt ttcagacaat tcaccttcaa actcatctga
     9181 gaaatacaaa aacatatacc aatcttcttc atctttaaat tttggaaaag ttatcaaccc
     9241 tttatatgac aatgatttaa gagtaagacc agtctcttcc tttacttccc taattacaca
     9301 ttcttctgga gtctctcctt gctcaaactt tcctccaact cctacatact taccttcatg
     9361 tatatcatct ttctttttat ttctgtacaa catcaaagtc ttgtcatctt tttctatata
     9421 acaaatagtt gtaagtttca tgtatctcgc cttactttct ctttatattc tcaattaatt
     9481 attttcagca ctttcaagca catcacttat attcttcatt gcattaagag aaagttctgc
     9541 aaattcatca aatggaatcc ctatatcttc tatacttctc attgcatctc tatttacatt
     9601 tgaagcaaat ctcgtttctt tcattctttt aataacagac ttagtcttta cactagtaag
     9661 ttttttatct ggataaacta aagctatagc agttataaac ccagttatag ggtctgctgc
     9721 atatattgct ttttccatta agctagttct tgtaactccc attccatcat gatgaccaag
     9781 tattgctcta tacatttctt cgtcaccgaa gttttctttt ttaagcagtt ctactgttgt
     9841 tgtagcatgt ccttcacata tttctcctgg ctttcttcca actatatctg aatccaaatc
     9901 atgtaaaaga cctgccactg cccactcttc ttctttttct ggctctagtc ttttggctaa
     9961 ttctctcata acagcttcaa cagcataaca atgttttctc attctatcag tttctaaata
    10021 cttaaataaa atttctaaag cttcttctct cattttcacc aatccccctg ttttttcaca
    10081 tatatgatat ttttatatat taatacacat aaattcaagt ctttatatat attcatgtat
    10141 atttttcatg ttatatctat tctacacctt tatagtagtg tcctgcaact gttccaattt
    10201 catctaattt cttatacaaa tctgtagcct tttttccaag tttataatta ttactcaata
    10261 cctcaatgtg agcttcaata acctttttaa ccatctcact atcttcaact gtaaaatcta
    10321 tcctaaatac atctattcca atttcctcta actcatatag attgtccaac atacaagtaa
    10381 ccttcgaatt gtaaattgta cttctacaga atatatcttg tgttatccta taacttgcat
    10441 ccgtaaagtc tcttaaagca taactacttt ccttacattt agcaactctc ttgtcctttt
    10501 tacaatctct aacaactact cccataggac agtattctgt aaccatcata ggagcatatc
    10561 catatattac tgcttcaatt tctacatctg tatattttaa agtttgttta atttcattta
    10621 agtttagttc ttgtgacaaa caaactgtac ttgctccctc ttctttgaag tggtttatac
    10681 tttcactatt gaatgcattt aagtagcaat ctatatttaa gtcttttttc ttaaagtatt
    10741 ccatagagcc ccagttacca atttgaacct tattaattcc ttttatatta tccaatatct
    10801 caaattgctt gtactcttta tttctaacaa tccttggtgc tgaatagatt aattttttgt
    10861 cagtgactgc tgtattatag tggttcttat tcaaagtatc tattttactt gtatttgaca
    10921 aagtatctat gttatttgta tttgaaccca ttagttcttt aggaatacct aaagacgaag
    10981 aagtatctaa atccatagaa atacttgaat ctatagaaat acttgaatct atagaaatac
    11041 ttgaatctat agaaatactt gaggccattt gtaaagcttt tgataatgta tttatatctt
    11101 cataatagat taaatctaca ttataattta atactgcttc taattgctcc aagttcttaa
    11161 ctttaactct aatcttagga actgtatcaa gttttttctg tttatttact ctatttggtt
    11221 ggtattttaa tctattcttc ttaaattttc tatcttttat ctttattctt tcttcatcta
    11281 aaagctcaat acaatccctt ctcatttgat ttaacacact tataggcata cttacacctt
    11341 catccaggtc aatttctatc tcttttaact tatatggtgt atttccaagc ttttgaattt
    11401 gtgtttctat tttttcttta cttaaagcca cctttatagc tttttcaact attttgtctc
    11461 cttctaccac cgatttattt ccacaaaaat cacttataaa aagctctggc tttttaccta
    11521 gtttaatact cacttttgca cgtatattag tttttatgaa ctctttatct tgctgaaatg
    11581 tagcttgtac tctattcatt aattcgctgt cagaagtctt atatataagc tgatttctct
    11641 ttgcttctcc aacaaaatct aattctatca aatccccttt atgtgctatt gtagttatct
    11701 ctccatcctt tattattctt ccaatagtac ctccacctaa gttaattcca tcacctttct
    11761 taagcgtatt ttctagcatt actctaagtc ttttgttctt tctattataa tctaaaactt
    11821 ttccaatata tagcccttga ttattaggtc tattagagtt cattatatcc ttaccaactt
    11881 cacctaatat atatcctttt gtaaactttc tattaaatat ggtatacaaa ttatttatta
    11941 tttcctcaga aatacttatt tttttcttat ctacaaattc attaatcgct tctctatatc
    12001 ctgaaaccac agtagctaca tactctggtt ttttcattct cccttcaatt tttaaagata
    12061 atacacctgt ttcaattatt ttatccaaat cttctatagt acttaaatct ctaggactca
    12121 acaaataatc tccattactt tttactactt ctcctgtatt tatatctatc aactcatact
    12181 tttgtctaca tggctgtgca cacctacctc tatttccaga cctattgcct atcatactac
    12241 tcataaggca ttgacccgag taacatacac ataaagctcc atgtacaaaa acttctatat
    12301 ctacagttgt attttcacaa atccatttta tctcatcaac atttaattct cttgctaaaa
    12361 caactctatc aaaccctaca ctctgaaggt attttacatc ttctaaggaa tgtgctacca
    12421 tttgagtgct tgcatgtagt tcaaagtcag gtaatagttt ttttataagt cttgccattc
    12481 ctatatcctg tactattaaa gcatcaatat ctatatcgta taaaaatttt acatactcta
    12541 caaaatcttc tatttcatac tgttttatca atgtatttac agtaacaaag acttgcactc
    12601 cccttatatg agagtattta acagcctcaa tcagctcatt tctatcaaag ttatttgcag
    12661 atgctctagc actaaagtct ttaccaccta aatacacagc atttgctccg ttttgaactg
    12721 ctgcttttaa tgaatcaaaa gagcctactg gtgcaagtaa ttctattttt ttcatatttt
    12781 tctcctctat ttatacttgt taggttaata taattatatc tataacttaa atatttgtat
    12841 taaatttata tatttcttac tatattttaa attcttcaat taatttatat actgcttact
    12901 aaatttaaaa cattttaatt aatttgtaca ctgtttacta tacttgaaat acttctatta
    12961 atctatatat tgcctactat acttaagaca ttttaattaa tttatacact gcttactata
    13021 cttaaaatac ttctattaac ttatatattg cttactatat tttaaattct ttaattaatc
    13081 tatacaatat ttgctatata taaagtatat caaataacag cttcattttt ttgtaaattt
    13141 acagaaagtt aattttcatt aaatgaatca tctttattga taaacataat tccaaagacc
    13201 actgccaaaa taaaaaaaca tattagtatt gatttttcca cgaaaatttt tgtaaaaaaa
    13261 gttcctaaaa tagctccaga tataaagaat ataataatag cataatactg taaactcttt
    13321 tttatcataa aaatattctt agtatgtata taatgaaata aagtttctgt agcacttctt
    13381 aaatttccag tacacattgt cgttgcaaaa ctattcccat ttacttttct aaaactctct
    13441 acttgcattg aacaaataaa agaaatagca acatttacaa acatattaaa tcttcctcta
    13501 ggaataaatg aaacaataag tattgtaatc atttcaatta taatgataac ctgacgccaa
    13561 tgaatctttg tcttattatt tgtaaaatac tttcttatct tctcggctat aaatacacct
    13621 aacataaatg ccaaaatagg aatcaaataa taaaatgatt cctttacatt accttctgct
    13681 atattaagcc caaataaaac tatatttcca gtttgtgcat tagcaaagac tttacctctt
    13741 accacataag tataaatatc caaaaatcct cctgttattg ccaacaaaga acataaaaag
    13801 aatgattcag acatctgact cttgcttgtc atatataaac ctctcgcttt ctaatgatag
    13861 atacaaaatc aaaatcattt tagattctat ttttcattga tataaaaaaa ttatttatat
    13921 ttaaatatta tctatattta tacatcattt atatttatac attatttgta tttatatatc
    13981 attcatattt atttatattt aaacattatc tatatctata tagcatttat atttatttac
    14041 atttaaacat tatctatatt tatatagcat ttatatttat ttacatttaa acattagcta
    14101 tatttatata gcatttatat ttatacatta aaagaagctg tctcaaaaaa caagagacag
    14161 caaatctttt agtttattcc acattttttg catcattatt taaccctgta cattttaact
    14221 gtaaatcata agctttgttt tgaaactcag aagctaagtt ttcagctata gtagctcttt
    14281 catttgcttc ttcaagttga gtacttaact cctcaatctt gtttttataa acatctaact
    14341 ctgctctaga accttctgtc gtattactaa gttcgaatat ttcttgcttt ttattttcta
    14401 taatctcaat taactctttc atcttagtct cagcttcacg attttctgct tctttttctg
    14461 ctatcgtaag ctttaaagac ttgatttcta attgaagctc ttcattagat gctccaacct
    14521 tcttactcaa ttcttcatta gctttaatca acttctcatt ctcatctgaa cattcaaata
    14581 aaaggtctga tatatttaat gcagttagta tcgcagcaac tgataagctc aatttcgggt
    14641 tttgacgtgt tattttatcc atttctttgt ctacaaaatc agcaatacta atcataaact
    14701 tctctgattt gtctccaacc attggatatt cagcgccatg gattttaacc attactttgt
    14761 tcatcctaac ctcccccgac attagttgtt aatttgatct taagttagca tttaattttt
    14821 cgcttaattc acttaatatc ttatcatgca ccttagctac atcttcatct gttagagttt
    14881 tatctttact tctataagtt attgaatacg caattgactt gtatccagct tctatttgag
    14941 aacctttgta aacatcaaat aacttgtagc tttctactaa tccttgacca ttttccttta
    15001 ttatatcttc tatttgtttt acaaatacat catctttaac aataagagct atatctcttg
    15061 atgtagatgg atattttggt aatggcacat aattctttgt tctatcagaa ttatcaaata
    15121 caaagtcaat atctatttca gcgacataaa ctctttgacc tagattgtag ttttctataa
    15181 catctggatg caattcacct aatgtaccta catatttatt attatacact attttagcac
    15241 atctaccagg gtggaaggta gtattattat cttgaggttc aatttcataa cctttaaatc
    15301 cgacatttac aagtatagtt tctatagttc cttttagtgc aaagaagtca acatctttac
    15361 catacatacc tacacacatt ctattttctt ctttaggtaa ttctgaatct tgtgggatga
    15421 atatatgtcc acactcaaat gcagatactt cttctatttt atgagataca ttagttgata
    15481 aaacatctaa catatttggt attaaagtag ttctcattac agaagtttct tctcctaatg
    15541 ggttaagtaa tttaacaaag tttctcttag cattaccttc tggtactctt atcttatcga
    15601 ctcccttagg gcttacaaat gaatatgtta atatttcatt taatcctaca gcagttgaat
    15661 tatcttttaa agcatccatg aatttttgtt ttgaagtttt aattcctgct gtcgtatttc
    15721 cttctaattg tacagaaggt atattctcaa atccataaat tctagctatt tcttcccata
    15781 catcagcttc ttgttccata tctgttctaa agcttggtac atctatttct aatttatcat
    15841 tagctaccaa attacattta aactctaatg attctaaaat tcctacaaac tgctccattg
    15901 gtacatctac acctaatagg tggttaattc tttgaggatt tactaccaat tttttaggtt
    15961 ctggtttatt tggatataca tctacaacac cttttaaaac ttttcctgct cctaacattt
    16021 caacaagttg tgcagctcta tttgctgcta tctctgcaag attagggtct aagtcttttt
    16081 catttcttga agatgcttct gacctaatac caactttttt agctgtcatt cttatgtttt
    16141 ctggtttgaa attggcactt tcaaaaagta cagtcttcgt attagaagtt atttctgagt
    16201 tagcaccacc cattacacca gctaaaccaa gtgatttttc tccatttgtt ataactagca
    16261 tatctttatc taatgttctc tcaacatcat ctaatgttac aagtttctct ccatctttag
    16321 catttcttac tactattctt ccagtctcta cttgatttat atcaaaagca tgaagtggtt
    16381 gaccaagctc taacattacg aagtttgtta tatcgactat gttacttata ggtcttactc
    16441 ctgcttctgt aagtcttctt tgcatccaat atggagaagg ttctattttt acatctgtaa
    16501 ccattctagc aacatatctt ctacataaat ctggattatc tatctcaact ttgaaatcta
    16561 tctcttcgtc actttctttt acttcgattt caggatattt tacttttgtt cctatagttg
    16621 cagctgcttc tctagctata cccatcatgc atttacaatc aggtctgttt gaagttaatt
    16681 caaaatctat taaagcatct tttaatccta aaacatcttt tatatctttt cctaattcat
    16741 aagaatcttc gtggtctaaa atatatatac cacctctttt atactcctca atgtaatgtt
    16801 catctatacc tagttcagct gctgaacaca tcataccatc tgataattca cctctgaaat
    16861 cagtctgttt gatttcaact cctccaggta acttagaacc atttacagct actggaatat
    16921 aatctccttc tgatatattt gtagctcctg taactatttg aacaacttta tctccaatat
    16981 ctactttagt tacaaccaac ttatcagcat ttggatgttg ttttatttct aaaatctttc
    17041 caaccaatat attttctatt tcttcaccat aataatctat tgtttcaact ttagttcctg
    17101 tcattgtcat tttatcagcg aactctttta catccatgtc tatatcaaca tagtctctaa
    17161 gccattttaa agatactaac atctacatac cctcctaatt aaaattgatt taagaatctc
    17221 atatcatttt cgaataataa tctaatatca tctatttcgt atttaagcat tgcaagtctt
    17281 tcaaccccaa ctccaaatgc aaatccactg taaacttctg ggtctattcc acaatttcta
    17341 agcacatttg gatgaaccat acctgaacct aatatttcta tccaaccttc atatttacac
    17401 attgggcaac ctttaccacc acatttgaaa caagtaacat caacctctgc acttggttct
    17461 gtaaatggga agttgtgagg tctaaactta gttttgatat cagaaccaaa caatttttca
    17521 acgaagatat ccatagttcc tttaaattct gccatagtaa catcttttcc aacaacaagc
    17581 ccttctatct gatggaacat tggtgagtgt gtagcatctg gcgagtctga cctaaaacat
    17641 ctacctggtg caattacttt tattggtaac tcttgacttc tcatagttct tacttgaact
    17701 ggagatgttt gagttctaag taatacccca tcattgatat agaatgtatc gctcatatct
    17761 cttgatggat ggtctttagg agcgtttaat gcgtcaaagt tgttttcaac agtctcaact
    17821 tctggccctt ctgctataga gaatcccatt ccgataaata tatctgttac ttcatctata
    17881 atttgagtta ttggatgctt cttcccaacc tttaaaactt ttcctggttg agtaacatct
    17941 atcacttcct cttttaattt tctttctttt tcaatagcat ttatttcttc ttttttagaa
    18001 tttatgctaa gttcaatgtt ttctcttacc tcattggcaa ccttaccaac tactggtctt
    18061 tcttcagcag ataatttacc catttcttta agtatggcag ttatctcacc tttttttcct
    18121 aagtacttaa ctcttaaact ttctacactt tctatgcttt gtgcttcttt tatttcagcc
    18181 aaagctgctt cacgtaaagc aagtaatttt tcttgcacac atatcaccct ttcgtttttt
    18241 tgtataaaaa aagcccctca atccctgtct agggacgaaa ggctatagtt tcgcggtacc
    18301 accctagtag tttaacaatt taaaaatcat caaaccacct ctaacaaact taacgcgttt
    18361 ttgacgtcaa agcctactgt aaaatttcag ctctgttact ccagagggaa cttctcatga
    18421 cctcttagaa aacactccca gcctaggtgt ttatctcttt actaagttta atcaaaatac
    18481 tttcctcttt cattgcattt acaatattat attgtttatt ttagtattat atcatttcca
    18541 ttcaaattat gtgacta
//
LOCUS       DAMJVX010000068        18419 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMJVX010000068 DAMJVX010000000
VERSION     DAMJVX010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.071"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            221..2977
                     /locus_tag="PC407_002036"
     CDS             221..2977
                     /locus_tag="PC407_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2497482.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSKFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNTILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEIFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALESKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINGPMLFIDQISTISALEVGNLLMQGKNIEQIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLPNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
     gene            3109..3588
                     /locus_tag="PC407_002037"
     CDS             3109..3588
                     /locus_tag="PC407_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2497483.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTRDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKEIGDLGIDIELRMLPRDKKKQLSDLIK"
     gene            3604..4026
                     /locus_tag="PC407_002038"
     CDS             3604..4026
                     /locus_tag="PC407_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDF2497484.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLANIEYFCMTKDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSIFL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENRNKIKNVM"
     gene            4066..4857
                     /locus_tag="PC407_002039"
     CDS             4066..4857
                     /locus_tag="PC407_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2497485.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMFDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTTLINAIPKVILDGLGVAGTLLPAVGFALLMDMLISKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAMNTLTEGEIDFE"
     gene            4850..5650
                     /locus_tag="PC407_002040"
     CDS             4850..5650
                     /locus_tag="PC407_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2497486.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     KVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFLIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            5698..6753
                     /locus_tag="PC407_002041"
     CDS             5698..6753
                     /locus_tag="PC407_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2497487.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRANLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGTISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(6913..8859)
                     /gene="feoB"
                     /locus_tag="PC407_002042"
     CDS             complement(6913..8859)
                     /gene="feoB"
                     /locus_tag="PC407_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2497488.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKSNTH"
     gene            complement(8877..9095)
                     /locus_tag="PC407_002043"
     CDS             complement(8877..9095)
                     /locus_tag="PC407_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2497489.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            9301..9810
                     /locus_tag="PC407_002044"
     CDS             9301..9810
                     /locus_tag="PC407_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDF2497490.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            9888..10049
                     /locus_tag="PC407_002045"
     CDS             9888..10049
                     /locus_tag="PC407_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HDF2497491.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            10200..10865
                     /locus_tag="PC407_002046"
     CDS             10200..10865
                     /locus_tag="PC407_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2497492.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            10931..12715
                     /gene="pepF"
                     /locus_tag="PC407_002047"
     CDS             10931..12715
                     /gene="pepF"
                     /locus_tag="PC407_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2497493.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEKYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            12967..13809
                     /locus_tag="PC407_002048"
     CDS             12967..13809
                     /locus_tag="PC407_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2497494.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVMANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     regulatory      14042..14125
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            14705..15382
                     /locus_tag="PC407_002049"
     CDS             14705..15382
                     /locus_tag="PC407_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2497495.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVERI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     gene            15454..17469
                     /locus_tag="PC407_002050"
     CDS             15454..17469
                     /locus_tag="PC407_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2497496.1"
                     /translation="MKKYNILKGTLIVLIVSLLAIAGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGEDKLLSLELPEIFSASEVWINGEIVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKDTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKLYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDSSY
                     FMVVLCIICINGSISKIEEDKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFIIVILFSLMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KKFLADVAHDLKAPVSAIQSFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNDFIKEIYENNLPDTQACGVNFETYILEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKVSDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            17519..18199
                     /locus_tag="PC407_002051"
     CDS             17519..18199
                     /locus_tag="PC407_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2497497.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKEISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILTDGDGWTLCKKIRNVTTCPIVYMTYINEEQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
BASE COUNT         7209 a         2033 c         3158 g         6019 t
ORIGIN      
        1 aatacaataa atatattctt atataaattc tatgtcagat atatggaaag ataagaacct
       61 tgagattatg ttaaaaaatc tagattttat acttataaaa agtttgattt tacaacctta
      121 caaaagagaa tcaagatagg aatataatta tctttttcat aaatatataa atgatatact
      181 aatattatag tattattgat atttgtattg gaggttgaac atgagggatg aaattttaag
      241 ttttgtaaat gaatgtgtat tatttaacaa ctttgatgaa cttaaaaaag aaagtggcat
      301 aataacagaa gatatagtaa aaaagtttaa tttaagtaga actcaatcaa gtagattatt
      361 aaatgattta gtaaaatctg ataatcttat aaagataaac agtagacctg ttattttttt
      421 acctaaaaat aaaataaatg atgtagtagg acctacaaaa aaaagtattt atgaaaattt
      481 agatgaactt aaaaatgagt gtaaattatt aaagcaagag actgtgttta atagtatcat
      541 aggatatgat aatagcttaa aagaagtaat cgaacaaata aaaaccgctg tactttatcc
      601 aaatggcgga cttacaataa tgctgacagg tgaaagtgga gtaggaaaaa cttttttagc
      661 caagataata tacaaatata gtgtaagcag taatgtatta gaaaaaaatt ctccatacaa
      721 tgtgttaaac tgtgcgcagt attataataa tttggagtta ttgtctagtc tattatttgg
      781 gcatgtaaaa ggagcatata ctggagcaaa tgaatctaga gcaggtctaa tagaacaatc
      841 agatggtggt gttttatttt tagatgaagt acatagatta aatagtgaag gtcaggaaaa
      901 gttatttaca tttatggata caggtacgtt ttctaggatt ggagagaatg gagtaattag
      961 aaaagctagg gtaagattaa tatttgcaac aacagaaagt ctatccaaat ttttgcaaac
     1021 ttttttaaga agaataccca taaatattta tgttcccaat atagaagaaa gagggactat
     1081 tgaaaaaaga aagattgtag aacattttat ttatgaagaa agtaaggtac ttggcttgcc
     1141 tatagagata actcaaatta cattaaatac gattttaaag ataaaattca aggggaatat
     1201 aggtgaatgt aaaaatgtaa taaaatatgc atgtggacga gcttatgcaa aaaatcaaag
     1261 aagaaatgga aatatttttg ttactcttca agacctccct agaaatcttt atatagaaaa
     1321 tgtagatata tttcaattcc aaagtggtaa aagcaaaagc attatctttt ctcctaaaca
     1381 atttagtatt gaaatgtcaa atggaagaaa taaaagaaat ataatctatc aaagtttcat
     1441 aaaatgttta gactactttg atagactaga aaaaaatgaa ataacaaaag aaatatttag
     1501 taaaaagagt tctaatctaa tgattagact aatggacaat ttagtctttt caaataatga
     1561 tgaaaataat aacgtactta tggagcttat aatatcgata atgcaggata tatttagaaa
     1621 tttagaagtt aataacaata taaaatacga tgggaataat gtattggcat tttcgtctta
     1681 tctttatttg aaggacgaat cttatcaatt atctgatgag tataaagctt tagaaagtaa
     1741 attactaata tatttaaaaa aagagagtta caaagaatac tctataataa gtaaaatatc
     1801 aacattaata acgaatagat tggatatgta tcttaataat gtagatacaa tcataatgat
     1861 tatatttatt aaatcaacaa ttataagaat gaaatttaat caagttaatg cgactattat
     1921 agcacatgga tatgctactg ccagtagtat ggcgaatgta tgtaatcgga tgttgggtaa
     1981 aaatatattt acagctattg atatgcctat agatgctacc attgctgatg tatcaaaaca
     2041 aatgtctaga tacctagaag gagataggat aaagaatggt ttaattgtgt tatttgatat
     2101 gggctctctt aatcttatct atgaagattt aaaaaataag ataaatgggc caatgttatt
     2161 tatagaccaa atatccacta tttctgcact agaagtagga aatttattaa tgcaaggtaa
     2221 aaacattgaa cagattgcag aaattatgga tgaagaaact aaacctaata tacaattgta
     2281 ttatcctcaa aaaaataaag aatatgctat aatatcatct tgttttacag ggataggtac
     2341 tgctataaaa atccaacatc tactttccga aagtttaatt gggatagttg atgtcaaaat
     2401 aataccttat gattatgata gtcttgtttc tatgggagat aaagaaccag tttttgaaat
     2461 gtatgatgtg atggctatag taggaactgc taatccaaat gtgaatgggg tggattttat
     2521 attgttagag gatataatat caggaaaagg agaagatgat gtttttagaa tatttagtag
     2581 agtagtagaa aatgaaaaaa taaagaacat aaatgattct attgtaaaga atttttcatt
     2641 aattagagta atagacagtc taactatttt agattcaaaa aagataatag agagaataga
     2701 agaaggaatc aatgctatag aaaatattca aaagaaaaaa cttccaaatg ataaaaagat
     2761 ttctttatat gttcatttaa gttgtatgat tgaaagatta gttagacaaa ctgaaattga
     2821 agagtatact gatatggatt tgttaatcaa aaaccatcat agtgaagtaa aactaataaa
     2881 aaatgcattt agtgtgctag aaaagagtta tagtgtgcaa ataccaattt cagagatagg
     2941 atatatttat aatattatct atggtctacc acagtaaaat acaagattac aaaataagta
     3001 aatgaaattt aaaatagtat gccagtattt ggcatacttt ttgcttttat agataaatga
     3061 gagtataaac aataaaaatt ttaatgtatg atggaggatt tggggaaaat ggtatctttg
     3121 atgagaattg atgacagatt gatacatggt caggttgcat atggatgggc aacatcacta
     3181 ggtataaatg taatactagt tgcaaatgat gaagcaaaaa atgaccaaat gaaagctatg
     3241 tcactgaatt tagcgaaacc tagcaatgta actttatata taaggggggt agaggaatct
     3301 ggagaaatag tagaaaaatt tgcttcttca aagaaaagca atgtattagt gttagtaaaa
     3361 aataccagag atgctttagt tgtagctaaa aattcaggag gagtattaaa agaaataaat
     3421 gtaggagggt taagatatga agaaggaaaa aagaaactta cagatttagt tgctgttgat
     3481 gatgatgata taaataactt taaagaaata ggtgatttag gaattgatat agaacttaga
     3541 atgttaccta gagacaaaaa gaaacaacta tcagatttaa taaaataatt taggagaaag
     3601 agaatgaaaa aatttctaat tgcaacacat ggagaattgt ctagagaact tgttgacaca
     3661 agtaaattaa taattggttc attagctaat attgagtatt tttgtatgac aaaagataaa
     3721 tcagcagatg atgctgagaa agaaattaga agtatattat caaataataa agatagtgaa
     3781 gaatttgttg ttttaacaga tgtgtttggt ggaagtgtgg caaatatatg tacaaactta
     3841 cttttgcaag ggttcaaatt tgaattgttg acaggcgtaa atttaccaat gcttttatct
     3901 atattcttat tagttgagga ggatacaaaa ctaatagtta aatcaggctt agaggaagca
     3961 aaaaatggaa ttgtttatgt aaatgagtta ttagaaaatc gaaataaaat caaaaatgta
     4021 atgtaattta atgtaaatta aactaagtta aaatggaggt tcaatatgtt agtacaagct
     4081 actatgttag ctattatagc aggagttggt atactagatg ggcgtatatt tggtcaatct
     4141 atgtttgata ggccaatagt tacaggtatg ttagttggat tagttttagg agacataaaa
     4201 tcaggaatta tgattggtgc acagcttgaa ttaatttgga tgggaattgc agggataggt
     4261 gcagctactc ctccagacgt agtaacagga ggtgtattgg gtacagcttt tgctatatta
     4321 tcaggtaatg gtgcagaagt tgctttagca gtggcagtgc cagtagcagt attagcacaa
     4381 tctcttggag tattagttag aataataaat tcgtactttt ctcaaaaggc agttttctat
     4441 gctaaacagg ctgattttaa gaaagttacc ataatgatgt ggttgccagt aatattattc
     4501 tttttaagta catcagttcc aaccttttta gcaattatga ttggtgctga taaagttaca
     4561 acattgataa atgcaatacc taaagttata ttagacggat tgggtgttgc aggaacattg
     4621 ttgccagcag taggttttgc gttgcttatg gatatgttga tttcaaaaaa gatgtctgta
     4681 tttttcttta tagggttttt gttagcatct tatggtggat tagatataac tgctatagct
     4741 ttatttggag tatgtgtagc atttgtacta aacttttata tcaataacaa agaaaataat
     4801 caaaatcctc aattagcaat gaatacttta acggaaggag agattgactt tgaataaatt
     4861 aacaaaaaaa gaattaagaa gtatgttttg gcgttcattt gctcttcaag gtgcatttaa
     4921 ttacgaaaga atgcaaaact taggttattg ttattcaatg ttacctgcaa taaagaagct
     4981 atataataag aaagaaaatc aagcaaaagc aatagaaagg catttagaga tttttaatac
     5041 aacgacagtg gtagttcctg ctatattagg tataacagca gctatggaag aagaaaatgc
     5101 taataatcca gaatttgatg aaagttcaat tagtgctgtt aaaacagctc ttatgggacc
     5161 tcttgcagga attggtgatt cattattctg gggaacattt aggattatag cagcaggagt
     5221 aggagtatca cttgcaaaag aaggcaatat ctttggacca ctattattct tattattata
     5281 caatatacca gcatttgccc ttagaatatt tggacttaag tatggttata aagtaggggt
     5341 aaattctctt gaaagaatac aaagagaggg acttatggag aagataatgt ctatggctac
     5401 aactgtcggt ttattcgttg taggaggaat ggttgcaact atgttgagta taactactcc
     5461 tttgaaattt aacttaaatg gtgcagaagt aattttacaa gatatactag ataaaataat
     5521 acctaatatg ttacctttaa cctttgcatt tttaatatat tacatgttga agagaaaggt
     5581 aagtgtaaca aaattaacta taggcactat agtaacagga atcgctttac atgctatagg
     5641 tttgttatag aaattaatta taatttattg aatatataga taaggaaggt gtataaaatg
     5701 acaatacaag attatatgtt agaaacacca gttagaatga gagaaattat atctaatgca
     5761 gatagcttat ttaatgaagt taagagagca aatctaaaaa agattataat aacaggttca
     5821 ggtactagct atcactcagg agtgcaagtg caaccatatt tacaaaattt attagatata
     5881 gatgttgtga aaatgtatcc atttatgata actgaagata cattcaagtt tgataatgaa
     5941 aataccctag tagtaggtgt atctcaaggt gggagtagtt attctacata caacgctatg
     6001 aagttagctg aagataaggg ttgtaaaata gcatctatgg caggttgtaa gaatgcttta
     6061 atagatgaga tttctgatta tatattaaca gtaaattgtg gtgaagaaaa atctggagct
     6121 aaaacaaaag gatattattg tacaaagtta aacttaatgt tattaggtct tcaaatagct
     6181 agagaaaaag gtacaatatc tagtgaaaag tacaatgagg aaataaataa gatattagat
     6241 gcaattaata gatttgaagc agtgtataag ctctctaaac aatggattga aagaaacaaa
     6301 gaaaagttag ttaattctaa agaaataaga ataataggtc actctgatat atatggagat
     6361 acattagaag cagcattaaa attattggaa acaatgagaa tacctgtaac tgggtatgag
     6421 tttgaagagt ttatacacgg tatatataat gctataaata gtgattctac tatatttata
     6481 ctagatacag gaaaagaacc tagagtaact aagatgattg atgtactttc tggttggacg
     6541 gaaaatgtat ttgctatagg tagagatgtt acagaaaatg ataaaaattt aaagattgat
     6601 atcacagata atccttacta tcaaacattt aattttatag tacctataca actaatatgt
     6661 ggggaaatac ctacattaag aggcgtagac ccaagtgttc caaaagatac taggttccac
     6721 atgaagttag gtagcaagaa actgaataag taacaaaaaa agaggttgtc tcaaaataga
     6781 gataagcctc tttttgactg ttgaaatgat gaaaatatat ttgattttat ataaaaaaga
     6841 gtgcctcatg aactaaaaag ttaattttga gacacccact tttatcttat tttaaactaa
     6901 aatatttata tattagtgtg tattactttt tttattgctg tcttccttac ttgagcaaga
     6961 attagcactt ggacatccag agcaaataga acatccacta cagacatttc cgttttttgt
     7021 agacttgaat ttattaaaca gaataaagag tgcaaggact ataattattc cacctatcaa
     7081 agcttccatt attgaattac ccataaatag tactccacct atgcttttaa ctataaaagc
     7141 cattatccat gctagtataa attgatatgc aaatgatgta aacatcattt tattgccata
     7201 ttcttttttc atagttgcaa gtgcagctat acatggagta tataaagcag taaatatcaa
     7261 gaatgtatat gcagtaactc cattaaatat tgttggtaag acaacagtta gattaccata
     7321 tagtatattt aaagagttta ctacgatttc ttttgctcca agaccactta aaatagcaac
     7381 tgaagttctc caatcatcaa aaccaagagg tttgaatata ggagatataa tatgacctag
     7441 agtagccaag aagcttacat ttatatcttc tgtatatcca aaaacattga atgaagaaag
     7501 tccccaaatt acgactgaca ttgcaaacat gacagtaaca actcttatca agaaaccttt
     7561 tgatttattc caagtatttt taataagtgc atctatagta gggattctat attctggtaa
     7621 ttctaatata aatggttcag cctctgtttt aaaggctgtt ttatttaaga ataatgcaac
     7681 tacaattgcc ataactatac ccaccaaata cagtgacgta gttactaaag cagcattatg
     7741 tggaaagaat atagctacaa atagtgcata tataggtagc tttgctccac aagtcataag
     7801 tggtgcaata agagctgtaa ctcttctatc tttttcgctt tccaatgttc tagttgccat
     7861 tatagctgga gaagaacatc ccatacccat aactataggt ataaaagctt taccagatag
     7921 accaaccttt ctcataactt tatccattaa aaatgctgtt cttgacatgt atccactatc
     7981 ttcaaacaac gatataccaa agaataatgt aaatattaaa gggaagaatg gtaaagtacc
     8041 accaacacct ccaattattc catcaactat aagtgattta aaccatgggc tagagttaga
     8101 taacatatca ctaacaggtg cagaaatata agcctctata agactagcaa atccctcttg
     8161 tagtggtcct ccgacccagt caaaggtaaa tttaaataag aggaacaata caccaataaa
     8221 tatcggatat gctaatatag gattcaagac tatattgtct atcttatcac tgatagattt
     8281 cttttgtgta tatgtagttt ttgtacatct cgataaaatg gattctaatt ttttatatgt
     8341 ttctgtttca cttccaaagt ttttctgata tataaaagct ttttgcgtag ctcccttaat
     8401 ctcttcttct atcctatcaa caccattttt cttttttgct ataattggaa caacaattac
     8461 tccaagttct tcacttaatt ttttggcatc tatattaact cctttagatt ctgcaatgtc
     8521 taacatatta agcaatataa ctataggctt gttaaattgc ataagttgag ttgttaagta
     8581 aaggtttctt gataaattag aagcatcaac tacatttact attacatcaa catcttcatt
     8641 ttctaaatat gatttagaaa ccttttcttc atttgagaaa gtatccattg catatatacc
     8701 aggtaaatcg acaaccttta tctcttttcc aagaaatcct tctttttttt ctatagtaac
     8761 accaggccag ttacctacat attgatttga acctgtaagt agattaaaaa cagtagtttt
     8821 accgacattt gggtttccta ataatgctac attaatcacg ggtagctcct cctctattat
     8881 tgtaaagaaa tatttttagc atcagatttt ctaatcgcaa ggtcaaagcc tctaaatcta
     8941 attataattg ggtctccaag tggggcaacc tttttaactt ctatttcagt actatttata
     9001 catccaagtg caagcaatct tttagataat ttaatatctc catctatact attaataaat
     9061 cctttttctc ctatttttaa atcgtaaaca gtcattatta acacctccta atgaaattca
     9121 ttatcattaa aattgtaaca agtatgatac aaaaagtcaa gatttataga ggtaattgca
     9181 cattgaaata actaatattt attctaaata gattaaaaat attttagtct aaattaggtg
     9241 tttttatata ttttgtatta taatgtaaga tgatagataa aatttggagg agttaactta
     9301 atgaaaacta gaaaaatgac atttttaggt cttatgatag ggtatagttt agctttatat
     9361 gtactagaga catatatacc aaatcctctt atagttatat ttccaggggc aaaattgggt
     9421 ttgagcaata ttataacttt aatttcatta atgttgttag gtgtcaaaga tactgttata
     9481 attgttacta tcagggtaat tttgtcatca atatttgcag gccctttatc atatctacta
     9541 tttagtatag gaggagctta tctgagtctt ttatttatgt atttagcgag taaaataaaa
     9601 gatttatcac taatagggat aagcattata ggtgcaatag ggcataatat aggtcaatta
     9661 atagttgcaa gtttaatcat agaaaatata cttgctatag ggtacttacc atttatgtta
     9721 atggcatcat tagtaactgg tatgtttgta ggcttagtat ccaggtatac aatacctaaa
     9781 ttaaagcatt tttatagtaa gttttattga cataatattg acaaaatata atattgtttg
     9841 ataaaatgta tataaaaata tacaaatttg taatagtggt gattattatg gcgaacaatg
     9901 aattagaagc gttaaaaaat gagattgaag cagttaggga agagataaat acatacatag
     9961 aatatccaga aatatttcaa gaagagttag tagaatcaag taagaaaata gatatattaa
    10021 taaataaata catgtaccta agcaaatagc ccacagttgt gggttttttt gtatcaaaaa
    10081 ttaacagatg gctatatttt acatatatta ataataatat taagatataa aatagtattt
    10141 ctgatgtgta aattatctgt aggacataag ttgtaattaa gtcaggaggg ttttatatta
    10201 tgattgatat aggaagtaag gagatagcaa taaggttact attagcacta ataataggag
    10261 gaataatagg cttagagaga gaaaaaataa gacagtttgc aggatttagg acacatatac
    10321 taattgcaat aggttcttgt attgtttcaa taacttcgat tcaattattc tttgattata
    10381 atttgtatac aaatgctgac ccagcaagaa tgcctgctca agtattatct ggaattggtt
    10441 ttttaggtgc aggagcaatt ttaaaaaata aaggtggagt aaaagggctt actacagctg
    10501 ctgggatgtg ggcaactgca tgtattggta tagcagttgg atatggttat tatgaattgg
    10561 caattctagg tggattattt gtaatgatag cattatttgt gcttaggata atcaatacgt
    10621 ttttatttag ccaagaaaag aatgtattga tattaaaatt aagcaatttg gactctatac
    10681 cttttttata tgataaattt gaaaaaaatc aacttatcgt aagaaatatg gaaatagaat
    10741 gtcgtaatga agaatactgg aaggttaatt tttttgtatc ttatgctaag agaatacact
    10801 tacacgacgt tatgaaagaa ataaatatga tagaaggagt aataaatgta gattatttgg
    10861 actaaaatat atacaatata ttttgtaaag tgaataaaat ataaaacaaa ttaaaataga
    10921 ggtgtatgat atgggaactg atagaagtac aatagaagaa aagtacaaat ggaagataga
    10981 taagatgtac tccagtaaag aggaaattga aaaagacata agtaaagtaa aaaatcttat
    11041 ccaagaagta aaagaatata aagggaagct ttctgagagt aaggagaact tatataaagt
    11101 attaaatatc tctgaaaatg catctaggat tgttcaaaat ttatatgtat atactcatat
    11161 gaaacaacat gaagatacta gaattaatga taatcaagga agagccacta agacagaaat
    11221 gttatctact gatttaggtg tggctacttc ttatatagtt ccagaaatac tggcgataga
    11281 agagaataag ttaagtgaat acttaaaaga tgagaagcta tccttttata caaaatatat
    11341 aaaggatata ttgagagata aaccacatac tttgagtgaa cgagaggaag aaatacttgc
    11401 agctacatca gaattatcga caattccaga aaatgtatac gatatgttag catatgcaga
    11461 tatggaattt cctgagatag aagatgagga gggtaataag gttaagcttt ctcattcaaa
    11521 ctactcttta tttataaaaa gtaagaatag tagagttaga aaagacgctt ttgaaggtga
    11581 attttcaaca tatgagaaat ataaaaatac atttgcctca acgctttatg gtggaataaa
    11641 atcagagata ttttacgcaa aaactagaaa gtataattca gcaatagaag cgtctttatt
    11701 ttcagatgat gtaagtttag atgtttacaa taatcttata tcagctatag ataaaagtat
    11761 tcctaatcta aataaatatg ttgagcttaa aaagaaattt ttaggattga atgaaataca
    11821 catgtatgat ttgtatgttc ctttgacaga taaatttgac atggatatac catatgataa
    11881 agcacaggat atcatattag aggcacttaa acctttagga gaaaaatact tgaaggtcat
    11941 aaaaagtgct tttgatgaag ggtggattga tgtatatgat aatgaaggta aaaaaggagg
    12001 agcttattct tggggaagtt atgattcaca tccttatata ttaatgagct ataaaaatga
    12061 ccttaattct ctattcactt taattcatga attagggcac tctgttcaca gtcattattc
    12121 aagaacaagt caaccatttt tatattcgga ctataagata ttcgttgcag aagttgcatc
    12181 tacattaaat gaattacttc ttataaatta tttattagaa aattctaagt ctaaggatga
    12241 aactatatac ttattaaatt attatttaga acaatttaga actacagttc atagacaaac
    12301 tatgtttgct gaatttgaaa aaattgtaca tcaaagggta gagagtggag aaccacttac
    12361 tgctgatgaa tttactaata tatactacaa attaaatgaa aagtattacg gaaaatctgc
    12421 aatagtagat aaacaaatag gaatagaatg ggcgagaata cctcattttt attcaaactt
    12481 ctatgtatat aagtatgcca cagggttttc tgctgcaagt gctttaagtc aacagatact
    12541 ttcagaagga agtactgcag tagatagata tataaacttc ttaaaaagtg gagggtcaga
    12601 atacccacta gaacaattaa aatctgctgg tgtagatatg actaaaaaag aatctataga
    12661 ggaagcctta aatgtatttg ctgaattagt taataaacta gaaaaagaat tataggatgt
    12721 gataggttga tagcttcaat gaagacttct gataatatga gttgtattgt gtttatatct
    12781 gcctaaagta actttaaaaa gttaatctaa ttaagaataa atcatataaa attaagtttt
    12841 tacatgtatt taacaatatt tacatttact taaccctgct ctaatattca tttaacaaag
    12901 aatctgtatt attaaaatat agaaaaatga caacaaaaaa ttaaaatata agcgggggta
    12961 agtaaaatgt ttaaaaagaa aataggcgtt cttttagcta gtacaatgtt agttggagct
    13021 ttagcagtag gatgtagttc taactcagga ggttcaggtg attcaggaga ttcaagcaaa
    13081 gtttctgttt caggttcaac ttcaataggt cctcttatgg aaactatcgg agaaaagttt
    13141 caagaaaaaa atcaaggtgt tagtgtagaa gtacaacaga ttggttcttc cggaggtata
    13201 aaaaatgcca tagaaggaac atctgaaata ggaatgagtt caagagattt aaaagatgaa
    13261 gaaaaaagtg ctggattaaa ggaaactaaa atagcaatag atggtatagc attgataact
    13321 aatactgcaa actcagtaaa aaacctgaca atggaccaag ttaaaggtat atatacagga
    13381 aagattacta actggaaaga agtaggagga aatgatgcac caatagtaat agtatcaaga
    13441 gaagaaggtt caggaactag agatggattc caagaaattg tagggtttga ttcagaagaa
    13501 ctaactaaag atgcacaaat aagtgatggt tcaggaaata taaaaactac agttgaagga
    13561 aatgaaaatg ctataggata tatatcattt ggatatgttg atgagaaagt aaatagttta
    13621 aaaataaatg atgttgaagc aactgatgca aatgtaatgg ctaataaata tccaatatca
    13681 agaccattta tattagttaa taaagaagat ggtttgtcag aaaaagggaa agctttaata
    13741 gattatatat taagtgatga aggacaagca cttgtttcag aaaaaggatt tattaaagtt
    13801 aatcaataac atgaattata atataaagta aacatataat accctgtgag ataagctcat
    13861 aaaaatgatg ctttaacttg cagggtattg tctattattg aatgtagaaa tataaatatc
    13921 tttagataaa ataggtattt tagtatctta tatctaaaaa tatggaaata ttgacaaaat
    13981 aatattacaa tgttagaata aaaaagaaat ttatgaaaat atataaaaga tttttaataa
    14041 aaatatttta gaaactgaga agtatatctt attattgggc atctggagat atatggagtt
    14101 agtggtgcaa ccggctatga atataatttt tatattgcat agtctttttt tgctttaaaa
    14161 ttaacaaaaa tgttgcaaaa tttgaaaata agagtattag tcgttaagat ttttattgat
    14221 aggtgaaatt ttggctttta aagtagccat ttgttggaaa agggaaattt tttaaaaagt
    14281 ataatactat tataataaga aaataaagtt tttataaaca ttgagatgca aaatataaac
    14341 cttttgtaac aatatattta ccaaaataat acaatttatt ttatattata accatataaa
    14401 tcatataaat caatactcaa actagccaat agacaagttt ctagaaaaat aataaattta
    14461 taagtgaaaa tgactttaag ctccattctc caacaatcgg agactttaat tagtatttaa
    14521 taataaatct taatgttcaa tacaatattc aagaattgtt caaaaatact taagtattat
    14581 agtctagttt gtaaagatat tataaaataa taatactagt tatatatgaa aataaaacat
    14641 tataatttgt aataattata aaacttgaga ttatgattaa aatacctttg aatggagtgg
    14701 tgtaatgtat aatttactgg ttgtagatga tgatgttgat attttaaata taaatcaaat
    14761 ttatttttct aatataggtt ttaatgtata tacatcagag actgctaaag atgctatgga
    14821 tatagtagag cgtatagttt tagactgtat aattttggat atttcattgc cagatataga
    14881 tggatataaa atgtgcaatg atataaaaga aaaggttaac atacctataa tatttttatc
    14941 taattatgag catgaagaag aaaaagttcg gggattttta gctggggggg atgattatat
    15001 aacaaaacct tatagtttaa aagaacttga acttagaata tatgctcgta tgaggcagta
    15061 caaaaatatt acatcagttc ctaatgtgtt aaagttttca gcgttagtaa taaatgtaaa
    15121 ttcaagaaaa gtaacttttg atggagaaag tatagattta actactatgg aatttgaaat
    15181 attattgttt ctagctgaaa acaaagaaca agtattttcg aagatagaga tatataacca
    15241 agtttggaaa atgcctgata ttggagatca acatacagtg caggttcata ttgcacagat
    15301 gagaaaaaaa attaattctt taagcaggga gcatcaatac attcaaaccg tatggggaaa
    15361 aggatataag tttgtaccat aagtgagata ctgtaattaa taaatagttc ttgttttatt
    15421 taatactact atgaaagaga tgtggttgat gcaatgaaaa agtataatat actaaaagga
    15481 actctcattg tattgatagt ctctctgttg gcaatagcag gattaaatct tctgtataaa
    15541 tatgataata aatatacata taagtctatt agagcaagta atggaatttt aactatagat
    15601 aatgatactt tcactaaagg taaacttgtc tttctgatag atgactggga gttttataat
    15661 cataaaatat ttaaaccgaa tgattttaca gagcaagata taaaaccaga atatgttttt
    15721 ataggtcaat atccagattt ttctatgaga aaagggaatc agtcaccata tgggaatgct
    15781 acttatcgta tgagaataaa aaatgaaggt gaggataagc tactaagttt agagcttcct
    15841 gagatttttt ccgcaagtga agtatggata aatggagaaa tagtttctaa attaggagat
    15901 gtaggaacag aaaaatatag acccaaaatt aaaaattcag tagtaagttt tattgcaaag
    15961 aaagatacag aaattataat caatgtatcg aatttttcac attattatag tggtttgtat
    16021 tatccgcctg cattaggaga aacaaaggac ataagtaata tgatgtttta tcgattactt
    16081 ttttatagta taatttgctt cacaactttt gcaatagcta tattttcgtt gtctgtatgg
    16141 tttctttcaa acaaaagtaa actttactta tattttggat gtatgtgtgt tttctttgcc
    16201 atccatgtat cctatccctt tgtacatttg ttaggactgc ctttggtaag ctttacatat
    16261 gcggttgaag attcgtccta ttttatggta gttttatgta ttatttgtat aaatggaagt
    16321 atatccaaaa tagaagaaga taaagtgtat agatttttaa ttttaccatt aggaattaca
    16381 atgagtatca tatctatagt gataccaatt attcttcttc catatgatat gtactttgtc
    16441 aacatatatg ggaaattaat agacatatat aaatatattg tatttatata tatattaatg
    16501 tcttcgctgg tttcaatata taaaaagaca tgctctgagc atataagttc ttatatttta
    16561 gtttcagtaa acactgtttt tggaattagc atattatttg atgcagttac tagtaataga
    16621 tttgagccta tatttacagg gtggcaaaca gaatattgtg gatttataat tgttatttta
    16681 ttttctctta tgatgataaa caggaataga ataataataa aagagaatga aaaacttaca
    16741 aggaatcttg aacatgaagt tgaagtgaga actaatgaac ttacaacatt gttaaatgaa
    16801 cgtaaaaaat tcttggcaga tgtggcacat gatttaaagg cacctgtttc agctatacaa
    16861 tcatttatag acttgataaa ggttggtaat attcatgtag atgaagagac aagaacctat
    16921 ctttatgcta taaatcaaaa gtcaaatgaa gtacaaaata gagtacgtag tttgcaagaa
    16981 tttacatcac aagataaaag cattggtatt agtgaaaaaa tatgtctaaa tgattttatt
    17041 aaagagatat atgaaaataa cttgccagat acacaagcat gtggtgtaaa ctttgaaact
    17101 tacatattgg agaaaaaaat atatgcaaag ataaataaag agatgcttac aagagcattt
    17161 gagaatttaa tttataatgc tttgacattt actccatttg atggaaagat aattttatca
    17221 atggataaag aagatggatt tgcaattatt aaagtttctg ataatggaaa aggtatacct
    17281 ccagaaaata taccaaaggt atttgacaga ttctttacag aaagagataa taatgagcca
    17341 aaagggcaag ggctagggtt atatattgtc aaatccatta taagagagca tggcggagaa
    17401 atctttgtag attcagaact aggaaaagga actgttttta caataaaatt aagtctatgt
    17461 tttttataat aatagcttat ttaatataaa agaataatga tatttgggag tgattataat
    17521 gcaaactaag atattgataa tagatggaga caaggataat tgccagaagc taaaaggatt
    17581 tctggaggaa aaagaaataa gtatagattt agcatataat tgtgaagaag ctattggaaa
    17641 aatatttagc aataaatatg atttaatatt tttagaaata attctaactg atggtgatgg
    17701 atggactctt tgtaaaaaaa ttagaaatgt aactacttgt ccaattgttt atatgactta
    17761 tataaatgaa gaacaaagca ttttaaatgc tttaaattct ggcggagatg attacttaat
    17821 aaagcctcta aatttagaaa tattatatgc aaaagtaaaa gctattttaa gaagaatgaa
    17881 ctcatatgta aataataatg aaaataatac aaaattagag gaatataata gaactagtgg
    17941 agtaataaag ttagaagaca aaataataaa attgactcca acagaaaata agctgttaaa
    18001 cttttttata gagaatcctg aaaagactct gactactaaa gagatatatg aacatgtatg
    18061 gatgaatgag tacttagagg ataactatag tgtggtagtt gcagtgaatg gattaagaaa
    18121 gaaaatagag aaagattata aaaatcctca gaaaattata acaataagag gagcaggata
    18181 ttattttaat aaagaataat aaatatatta atctcattaa taattgttat aaatgtgaaa
    18241 tgctaacgat atctaaacta aattattata aaatagctga ctgtaattta attgataaag
    18301 cctattcttc tagactttgc cagttaaatt acagtcagtt ttattatatg aaaaaatgcg
    18361 taacagaact attattttat agattatatt tgaaaagata gataaattgt atatctaaa
//
LOCUS       DAMJVX010000069        18285 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMJVX010000069 DAMJVX010000000
VERSION     DAMJVX010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18285
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.072"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            157..1350
                     /locus_tag="PC407_002052"
     CDS             157..1350
                     /locus_tag="PC407_002052"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDF2497498.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            1556..2674
                     /locus_tag="PC407_002053"
     CDS             1556..2674
                     /locus_tag="PC407_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HDF2497499.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            2805..3050
                     /locus_tag="PC407_002054"
     CDS             2805..3050
                     /locus_tag="PC407_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HDF2497500.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            3037..3783
                     /locus_tag="PC407_002055"
     CDS             3037..3783
                     /locus_tag="PC407_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2497501.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            4068..4943
                     /gene="hslO"
                     /locus_tag="PC407_002056"
     CDS             4068..4943
                     /gene="hslO"
                     /locus_tag="PC407_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDF2497502.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPDDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            5334..6134
                     /locus_tag="PC407_002057"
     CDS             5334..6134
                     /locus_tag="PC407_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2497503.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            6661..8901
                     /locus_tag="PC407_002058"
     CDS             6661..8901
                     /locus_tag="PC407_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2497504.1"
                     /translation="MSNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNDIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSRDGEISEATKKKLSKLLIEDRCPICDGRRLNEGVYRSLI
                     NGYNIADLTSMQIDELAEVIKSINEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHVVDVGPKAGKYGGKVVYEGSYKNLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEIKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQRKNIIEVLEMSVLDAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            9315..10850
                     /locus_tag="PC407_002059"
     CDS             9315..10850
                     /locus_tag="PC407_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2497505.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTAMTISGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGIGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNISDLKNVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVIVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSEVFKMVGDILTDKEMAKMNYDVENNNLEPREVACEFLNKK
                     GLL"
     gene            10866..11627
                     /locus_tag="PC407_002060"
     CDS             10866..11627
                     /locus_tag="PC407_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2497506.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            11965..12833
                     /locus_tag="PC407_002061"
                     /pseudo
     CDS             11965..12833
                     /locus_tag="PC407_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            13027..13278
                     /locus_tag="PC407_002062"
     CDS             13027..13278
                     /locus_tag="PC407_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HDF2497507.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            13390..14169
                     /locus_tag="PC407_002063"
     CDS             13390..14169
                     /locus_tag="PC407_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2497508.1"
                     /translation="MNNTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(14304..14954)
                     /locus_tag="PC407_002064"
     CDS             complement(14304..14954)
                     /locus_tag="PC407_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497509.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIAFPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIDHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(14951..15454)
                     /locus_tag="PC407_002065"
     CDS             complement(14951..15454)
                     /locus_tag="PC407_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2497510.1"
                     /translation="MKLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGRFGCWI
                     KTPSECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(15503..16018)
                     /locus_tag="PC407_002066"
     CDS             complement(15503..16018)
                     /locus_tag="PC407_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2497511.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKIDVEENHCIDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            16334..16825
                     /locus_tag="PC407_002067"
     CDS             16334..16825
                     /locus_tag="PC407_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2497512.1"
                     /translation="MRTIDIKGENMRELLKWVSIINRYNTNYIDRKLADIGINNSQYF
                     YVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVKDKRTWHLYPTE
                     KSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMNEEAKLEAKENG
                     RKI"
     gene            16809..18212
                     /locus_tag="PC407_002068"
     CDS             16809..18212
                     /locus_tag="PC407_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2497513.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
BASE COUNT         6999 a         1948 c         3307 g         6031 t
ORIGIN      
        1 aatatgcata ttaatttaat aaaatatacc aaaatggaaa taaaaaataa attttatgag
       61 aatttaataa ctaataatta atatatttag gttataataa atatgtgcgt aaaatctaac
      121 aataagcaat aataccgtaa atttaggagg aataatatgg atactttaaa ggaacttttt
      181 aaaataggaa gtggaccttc aagttctcat actatgggac cacaaagagc agcagagaga
      241 tttaagaatg aaaacccaga tgcagaaagt tttagagcaa tattatatgg tagtttggca
      301 gctacaggga aaggtcactt aactgactat ataattgaaa aaactatagc acctaaaaaa
      361 gtcgaaattg tttgggaaga agatattata aaagattttc acccaaatgg gatgaagttt
      421 gaagctttag ataaagataa aaatgtaaca gctgaatgga ctgtatattc tgtaggtgga
      481 gggactatag cagaagaagg tcaaagaaac agtaaaagca atagtatata tcacttagat
      541 actatggatg agatagtaaa atggtgcaaa gaaaataata agactttggt agattttgtg
      601 ttggaatgtg aaccaaaaga cataaaagat tatataaaaa ctataaaaga tgctatgaga
      661 aaatctattg atgatggact aagtacagat gaaataatac caggcaagtt attattaaaa
      721 agaagagcaa gtacatttta taatgcatat aaaaaagata agagcttctc aacacttgta
      781 tatgcatatg cactagcagc ttcagagcaa aacgcttctg gtaatataat agttacagca
      841 ccaacttgtg gttctgctgg agtaatacca ggaatattct ttgcaatgca ggatttctat
      901 aattatgatg acgaaaaaat aattgaagca ctacttgtag ctggaataat aggtaatata
      961 ataaaaacta atgcttcaat atctggagca gaggtagggt gtcaaggtga agttggagca
     1021 gcttgttcta tggcagcagc agcagtagca tacttaaaag gtggaactat agaccatatt
     1081 gaatatgcgg cagaaatagc acttgaacat catttaggaa tgacttgtga ccctgtatat
     1141 ggatatgttc aaattccatg tatagaaaga aatgcaatgg cagctcaaag agcttatgat
     1201 gcagctaatt atgcattatt aactgatggc tcacattctg tctccttaga ccaagttgtt
     1261 gaaacaatga aagaaactgg tatagatatg atggataagt acaaagaaac agctaagggt
     1321 ggacttgcta aacatttctt ctcttgctaa aaataaaatt ttccaataat aattcctctg
     1381 ttttgattta tgaaacaggg gaattattat taatggtgaa ttatttaata atgcaaaata
     1441 tttttatatt caatttagct ttttattttt aattagtata acatgctata taatatttta
     1501 attataaaga aatatagaaa tattttttaa taaattagaa attggaggag attacatgag
     1561 agaggcatca tatttacaag aacaacttta taaacatagg aagtcattaa atcctattgc
     1621 agagattggg agaaaagagt ataagacttc aaaatatata agagattatc ttgataaatt
     1681 aggaattgag tatgaagttt atttagaaac aggtatagta ggtgtaatag aaggtaaaag
     1741 acctaaaaaa gatattgctt ttagagctga catagatggt cttcatagtg aagaaggggt
     1801 aaaacattta tgtggtcatg atggacatgc tactatatta cttggattaa ttgagtattt
     1861 aaatgataat aaagaagact taaatgataa tatagtattt atattccaac cagcagaaga
     1921 aggtcctggg ggagcaaaag ctttaataga tgaaggagtg cttagaaaat acaatattga
     1981 tgaaatatat gggcttcata tatatccaga gttaccagaa ggttatgtag gtacaagaga
     2041 aggctatttt atggctcaaa taggagatat cgacatagaa atagtgtcta aaagtggaca
     2101 tggggctatg ccacaaaatg gtattgatgg tatagtgata gcttcaaact ttgtaagctc
     2161 acttcaaact atagtgtcta gaaatataag cccaatagac aatgcagtgt tgactattgg
     2221 tagaattgaa gggggagcta gaagaaatat aatagctgaa aatataaaga tggaaggtac
     2281 tatgagatgt ttcagcccag aagtgtatga gaaaatgaag cttagagtta gagaacttgc
     2341 tagagggttt gaattggctt ataactgtaa ggttaatata aatattatag atgattatat
     2401 tgctgttaaa aatgacaagg atttgtatca agagtttgta gaggctattg gaagtgatac
     2461 tgttgtagag ttagagccac ttatgatatc agaagatttt tcttattatc aaaaagaagt
     2521 tccaggatta ttttttatgt tgggttcaag aaacgaagaa aaaggatttg taaatggtct
     2581 tcataatata aactttaatt ttgatgaaaa gatatgtgta aatgcattga atgtttattc
     2641 aaaattatta aaatacaaag aagcaataga ttaggaagat aaattataaa gattaaaaag
     2701 cataaatacc taaatttaaa aaaaatgtat taataaatac cccttatata tttgtataaa
     2761 tttattttaa ttgacaaaaa tacaaataag aaggggtgat ttttttgaaa gaaaaagtac
     2821 aaagagaaaa gaagataact aatggcaaga atactaaaat attgacaaat gatgatataa
     2881 tgaaatatga gatagcctca gaactaggtc ttatggataa agttggtgaa cgtggatggg
     2941 caggacttac agctaaggaa gcaggaagaa ttggaggtat gcttacatca cgcaaaaaga
     3001 ggcttaaaaa gattgtggag gagcagaata aaaaggatga gtcaatatag tgattttgcg
     3061 tttgtatatg atgaacttat gaatgaagtt gattataatg gttgggttaa atatatagaa
     3121 gatataatag atagtgaagg tgtaaaagta aaaaatatat tggaattggc atgtggcacg
     3181 ggtaatctta caattccatt gactaaaaaa aattacgata tagctggtat agatatatct
     3241 gatgagatgc ttagtgtagc aagagaaaag gcagaaaaag aaggagtaga attagtttta
     3301 cttcaacaag atatatcaga gcttgatttt gaaatatctg atttggactg tgtgctttgt
     3361 gcttgtgatg gatttaatta tataacttat gatgatgatt tggaaaatgt attttcaaag
     3421 acatatgaat tattaaagaa agatggaata ttcatatttg atataagttc tttttataaa
     3481 ttagccaaca tattgggaaa taacatgtat ggagaaaata gagaagatat agcgtatatg
     3541 tggcaaaatt actttgatga agaagagaat ctaattgaaa tggaacttac attctttatt
     3601 aaagatgaat gtggaaagtt tgaaagattt gaagagatac accaacaaag agcatatact
     3661 gaagatgaga tagttgatat gcttgaaaaa tctggattta aggatataaa atattatggg
     3721 gattttacat ttgacaaacc cagtgaagaa agtcaaagaa tcttctttgt atgtaaaaaa
     3781 taagatattt ttgactatat aagataatta ttaaaaataa ccatgatata aaaatataga
     3841 aaatgcagtt ttatagatta aaagtaaagt gatgatactt tatgactttt attttctaat
     3901 aattataaag ttctatagat aaaaattaaa atataataac tattgcttag taaaattaaa
     3961 ataaaatggg ttttgcccaa ggttaatata gataagtact tatagattaa aattaaggta
     4021 gtaatataat aaaaataaga caattaacgg gacatggagg aatattaatg agagattatg
     4081 tactaagagc gacatcagga aatggtcaag ttagagcatt tgtcgcaact actagaaata
     4141 cagtagaaga agctagaaga ttacatgaaa ctacaaaagt agcaacagca gcacttggaa
     4201 gaacattaac agccacatct ataatgggat taatgatgaa aaatgatagt gataaattga
     4261 ctgtaataat aaaaggtgga ggtccaattg gaactattat cgccacatca gattctaagg
     4321 gaatggtaaa aggatatgta ggaaatccac aagtagaggt tgaagactat ccaaatggaa
     4381 agttaaatgt agcagcagca gtaggtacag aaggtgttgt aaaagttata aaagacttag
     4441 gtcttagaga accatataat gggacttatc cattggtaag tggagaaatt gcagaagact
     4501 ttacttatta ttttgctgta tcagagcaga ctccatcagt agtagcttta ggtgttctta
     4561 caaaagaaga tgaagtagag tttgctggag gatttatagt tcaattaatg ccagatgcag
     4621 aagaagagac tattgctaag ttagaggaaa atgtagctaa attaccttct ataactaaca
     4681 tgttaaaaga aggaaaatca ccagatgata tattaaatat tgttttggat ggtttggaac
     4741 ctaaaatact tgatacctgt gaagtagggt ttatgtgtga atgttcaaaa gaaagagtaa
     4801 aaactgcttt ggttgcaatt ggtaaaaaat cattggctca gataatagaa gaagataaaa
     4861 aagcagaagt tggttgccag ttctgtaata agaagtatat gtatagtgaa gaagagttgc
     4921 ttgaaatttt aaaggaaatg taggctataa aaatagttgt attgatataa gtaatgttat
     4981 agtgatatag taaagtcgta ttgatatagt aattaaagta taatttatta ataatagaca
     5041 tatttatatt aaaaaataaa tttaataaat ttttaagctt aataaatttt aagaattaag
     5101 aagctttctg gctgtaatat ttgcaatgtt gcagttagaa ggctttttta ttttattata
     5161 gttcgtgtca aagattgtat gaaacaataa aatattgtaa aataatttgt atatttagac
     5221 ctgattgaca caaaggttta taagatattt gagttgatta tacgaaattc tatgaagtag
     5281 taaccctgtg actttaatca tgggaggttc agaagaagga gaaaatttta aatatggaaa
     5341 gtaaaacgaa gtacatagga ataagtatat taatattatt gatgatattt ttaattgcaa
     5401 agaattttaa cggaaaactt gaaataccta ttcctgagag taatagtaga aaatttaaat
     5461 cagcagcagt gttttatcct caacatcaag atgatgaggt tttatgggga ggaagtgcca
     5521 ttgtagatgc tataaaacag tgtggtgttg ataatgtata tgtggtatta gtatcagatg
     5581 gttctggagt taacgttttc aaagctaaca caaaatttag aaacttaact aggaagcaaa
     5641 aggaagagct tagaaataat gagtttaaat cagctttgag agagcttgga gtgaaacctc
     5701 aaaatgtgat tatacttgca gatatagata aaaaggaagg cacacattat gaattaatgg
     5761 aaaagactat attggatttt gaacataagt ttaaaggcaa tgtgacccat atagcccatc
     5821 actataagta tgatgaccat ataatgcata gaaagaatgg ggaagtttta aagaaactta
     5881 aacgtgaagg taagataaaa gatgacttgt attttgttaa gccaaaatat gtaaagtata
     5941 taccaagaaa tgaaagagct atctataaag caatggattt tgatgactac aataaggtta
     6001 agagtgcttg ttatgaatat aaggttgtaa attcaaagcg taaaatgtat ggtataggat
     6061 atatttcggc acatagttat tttgaacatc ttctaaatga ccctgaactt acgtctgtat
     6121 taagtgaaga ttaatttctt ataagtagta agttatgttt gataagttga ttggaatttt
     6181 ctatagtttt gaaggtattg gaaatttagt tttatttaaa gatttaaaga tagaggtttt
     6241 taatgtttaa aattttaaag ttaagttgaa taattgtata gaattatttg tgaaagaaga
     6301 aacagtgtaa aacatagtat cttatttatg gttttaaaat cataggcaag atactttttt
     6361 tatgtaacta ataccatttt gaggttatta acttaagaat tttatataat ttaacactca
     6421 tatataatga tgatatttag gtagaataga tgcaattacg cttaaataaa ataactgtat
     6481 tttttataaa aatcaaaccc tacactatac taccagataa aatctacatg ctataatttg
     6541 acctgtgttt ataaaaaaag gagtaatcta ataaaatagt atttcactta tgtataacta
     6601 acaactatta ttacgatttt ctctaaataa tttaaagaaa aataatgcag gaggtaaaat
     6661 atgagcaatg aatttataat tttaaaaggt tgcaaagaga ataatttgaa caatatttcc
     6721 ttaaaaatac caaaaagaaa aataaccata ttcacagggg tgtcgggttc tggaaagtca
     6781 tctattgtat ttgagacaat tgcaaaagaa tcacaacgcc aactaaatga aaggtttagt
     6841 acatttgtaa gaagtttttt acctaagtat ggagaggtta aagctgattg tatagaaaat
     6901 ctgtcaacac caattatcat agaccaaagt agacttggtg gaaattcaag gtcaacccta
     6961 ggtactatta cagatgtaaa ttccttttta agagctttat attcaaggtt tggtagtgag
     7021 tatataggta atgctaatat gttttctttt aatgatattg atggtatgtg cccacactgt
     7081 catgggcttg gaaaaaagct agttcctaat atgaaccaaa ttcttgatat gaataaatca
     7141 ttaaatgaag gtgctatatt gctttcaggg tttggagtgg gttcttggca ttggaatata
     7201 ttcgctgaat caggattttt tgatattaat aagaggatat gtgattattc agaagtagaa
     7261 ttggaaaagt ttttgcatgg taaagcagaa aagattaaga tagaaaatgc tggtcaaact
     7321 aacatgacat atgagggatt aatggttaaa tttaatcgtc tatacttaag tagagatgga
     7381 gaaatatctg aggctactaa gaaaaaattg agtaaacttc ttatagaaga tagatgtcct
     7441 atatgtgatg gaagaagact taatgaagga gtatataggt ctttaattaa tggttataat
     7501 atagcagatt taactagtat gcaaattgat gaattggctg aagttattaa aagtataaat
     7561 gagccagaag cacaaccttt aattaaagga ataatagaaa agttaaacag tatcatagat
     7621 ataggtcttg gatatttaac actggataga gagacatctt ctctatcagg aggggaatct
     7681 cagcgtataa agatggttaa gtatttaaat agtaatcttg ttgatttaat gtatattttt
     7741 gatgaaccaa gtgtaggact tcatccaaaa gatgtgtata aattaaataa tcttttaaaa
     7801 aaattgcgtg ataaagggaa tacaattata gttgttgaac atgacccaga tgttattaag
     7861 attgcagacc atgttgtaga tgtaggacca aaggcaggta agtatggtgg aaaagttgtg
     7921 tatgaaggca gttataaaaa tctacttaca agtggtactt tgacagggaa tgctttaagt
     7981 aaatttcttc ctattaaaga aagtgtaaga gaacatagtg gatatttaga aattaaaaat
     8041 tgtaataaaa acaatttaaa aaatgtatct attaagatac ctaaaggtgt attgactcta
     8101 gttactggag tagcaggagc aggtaaaagt acgttgatta aggatgaatt tttaaagcaa
     8161 aatcctagtg ctgtacttat agaccaaagc cctgtttcag caaattcaag gtcgagtttg
     8221 gcaacatata gtggaattat gaacaacata agaagcatat tttcaaaaac aaatggcgta
     8281 aatgcttctt tattcagttc aaattcagaa ggagcttgtg ataactgtaa gggttctgga
     8341 atagtagaaa tgaatcttgc ttttatggag agtataaaga gtacctgcaa tgtttgtgaa
     8401 ggtaaaaaat ttaaaaaaga agttttagaa tataggtttc aaaggaagaa tataattgaa
     8461 gttcttgaaa tgtctgtttt agacgcaatt gagtttttta atttaaaaca gattaaaacg
     8521 aaattacaat gtattgaaaa aatgggtatt ggatacctta ctttaggaca aactcttgat
     8581 actttgtctg gaggagagtg tcaaagatta aagcttgcta gtgaattaaa taaggaaagt
     8641 tcagtgtata tattagatga accaacaaca ggtcttcata tggctgatat tgagaatttt
     8701 ataagtataa ttgaagagat tgtagataat ggaaatacgg tcataataat tgaacacaat
     8761 atagatgtaa ttaaaagagc tgattggatt attgagctgg gacctgaagg agggacaaaa
     8821 ggcgggagag taatatttga aggtatccca aaacaattat gtaacaataa aatatcatta
     8881 acagcaaaat atatagtata ataaataaaa tataataaaa ttataatgat attttaataa
     8941 atataatggg tatataaaat gataattaaa cttaacagta ataaataatg tgagaaatat
     9001 tcaaatttaa ttaaataaag tgtcagaaat ttgtttaaat tttagtattt taacgattct
     9061 gacctttttt cttattttta tggagagtat cgaagatgga gtctctaata gttacttttt
     9121 tgtgtctact tgcaagtttg attaaaaaat ggctatacac acttgtgact tcagtcatgg
     9181 gaggttcaga ttataatctt aatatataat ttgtgaaata atttgatgac gatttgctga
     9241 tgtataaaca ataaagatta actacattaa aatttaaatc ttgttaagta aaacttataa
     9301 aaggagagag atggatgata aatgatattc taaccttatt atctgaaaga agtgattttt
     9361 ttataaagct atccatagaa catttgatga tttctattat ttctataata attgcgattg
     9421 ttttgggtgg tataattggt attattgtaa gtgaatttca taattcttct aaacctatat
     9481 tgggaactat caattttcta tacaccattc catctatttc tatgctagga tttctgatac
     9541 cattttccgg aattggaaat actactgctg ttattgcact taccatttat gcattattgc
     9601 caatggttag aaatacatat actggaatta ctaatgtgga taaaaatatt cttgaagcag
     9661 ctaggggaat gggcagtaca gattttcaga tattgtataa aataaaactt ccattagccc
     9721 tacctgttat tatgtctggt attagaaata tggtgattat gacaattgca ttagctggta
     9781 tcgcttcatt tataggtgct ggaggcctag gtgtagccat atatagagga atcacgacaa
     9841 ataacacagc aatgacaatt agtggaagta tacttattgc tctattagct ttaatatttg
     9901 attttatttt agggctggta gaaaaatttt ttcaacgaaa aagaaaaaag aaaaatagga
     9961 aaaaagtttt tgccattcta ggaagcctta ttcttatagc aactatcttt acgtcttatt
    10021 tatttaatcc ttcaaagact caaactattc atattgctac taaacctatg actgaacaat
    10081 atattcttgg tgagatgttg gatattttaa ttgaaaaaaa cacaggtcta aacgtggaaa
    10141 ttacacaagg cataggaggg ggaacatcta atatccagcc agctatggag aatggagaat
    10201 ttgacattta tccagagtat acaggtacag gctggaatat ggtacttaag aaaaatgaat
    10261 tatatactga aagtatgttt agtaaactac agtcggaata caaaactaaa cttaaaatgc
    10321 aatggactgg tatgtatggt ttttctaata gctatgcatt agcagttaga aaagaagttg
    10381 cagataaata taatttaaaa aatatatcag acttaaaaaa tgtatcttct aaattgacat
    10441 ttggagctga atatgacttt tatgagagag aggatggata tactccattg tgtaatacat
    10501 atggactgaa ctttaaagat acagttgatt tagatattgg attaaaatac caagctatca
    10561 atgaaggtaa gattgacgta atgcctatat ttacaactga tggtcagtat agtgtttcag
    10621 atgttatagt tctaaaagat gataaaaatt tctacccatc ttatcaatgt ggaaatgttg
    10681 ttcgtttgga agtattagag gaaaatccag aactatcaga ggtatttaag atggttggag
    10741 atattttgac tgataaagaa atggctaaaa tgaattatga cgtagaaaac aacaatttag
    10801 aacctagaga agttgcttgt gagtttctga ataagaaagg tcttttataa attggaggaa
    10861 atataatgac acctattata caatttaaaa atattaaaaa acaatataat gataaaacta
    10921 taatagataa tcttaattta gatatagaaa aaggagagtt tttgactgta attggttcgt
    10981 ctggttctgg taagacaaca ctcttaaaaa tgattaatgg tctgattttg ccagatggag
    11041 gaaatatttt gataaaccaa acagatatca aaaacgagga cttaattaaa ttaagaagaa
    11101 gaataggata ttgtgtacaa ggaagtgttc tttttcctca tatgactgtt gaagaaaata
    11161 tttcatatgt accaaatctc ctaagtaaaa agaataaatt agaagtaaaa agtgctgtaa
    11221 acaagtggat ggaaatagtg ggattgccaa atgatatgaa agtgcgttac ccatcagaat
    11281 tgtcaggtgg tcagcaacaa cgtgtaggga ttgcaagagc tttagcttct tctccagaaa
    11341 ttttattaat ggatgaacct tttggtgcag ttgatgagat tacacgaaag caattacaaa
    11401 aagagataaa agaaattcat aaaaaaacag gagttacgat tatctttatt acgcatgata
    11461 tttatgaagc tttgattttg ggaacaaaga ctttggtatt aaatcatgga gttgttcaac
    11521 agtatgatac tcctgagaat attttaaata ctcctgctaa ccaatttgta gaccagttat
    11581 tgaatataag aaaatctata atagatgatg ataatttaaa agaataaatt taaatttaaa
    11641 atcaaattgt tgtgataaaa actattgatt tagagctaac ttcaagttgt atacttgaag
    11701 tggaaaagtt agattaaggt atagaagaga atttagcagt tggctgtaga cgaggtaact
    11761 gctgaaggtg ctttcaataa atggctagaa cctgtatcta ttgaagatta taataaattg
    11821 aaataagaag ctcaatatac agagtagata ggcacttcaa atagaagtgt cttttgtgtg
    11881 ccataaaata aatcaaaata ttagcaaaga atgtgaaaaa caaaataaaa taatatgcta
    11941 tctttaaaat aggaggggtg ctatatggat aacaatttga ttaccattga aaaaacttta
    12001 gattatatag aaaaacatct tttagatgag atgttagatt taaatgtaat ttctaaagag
    12061 ataggctatt caaagtacca tttacataga atgtttacat cagttgtcgg atttagtata
    12121 cataattata tacaaagacg tagactaact gaagcagcaa gaatgctcat ttttacagaa
    12181 aaaccaatta ttgaaattgc tttaatctcg gggtatgaca cacaacgttc tttttcaaca
    12241 gggtttaaaa aaatgtttaa gcatagccct aagtttttta gaaaacatca agatttttta
    12301 ccactacaat taaaatttga tattaataat agaaaaaggt tacatggagg tatggtttta
    12361 gatgttaaaa aagttaattg tacagatata aatattgttg gctattctgg taaagtaaaa
    12421 aaaggatttc atgttattgg aaaatgttgg agggatttgc ataaaaataa agagttgata
    12481 gaaaacaagg tagacttgga ttttttaatt ggagttaatg attatagtaa ttttaaagaa
    12541 attgaaaata atcctacatt tacatatatt gcaggtgttc aagtagctac ttttggtaaa
    12601 ttcctaatgg aatggaaaaa ttcgtattac cgaaaagtaa ttatattgtt tttactttta
    12661 ttgggaaaaa tgaggatagt atgcaaaatg ttgtagaata tatctatcaa gaatggttcc
    12721 ttaattcaag ttgccagttt aatgaaaata atttatatga ttttgtaaaa tacggagaaa
    12781 aaactgataa gaatggtttg agtgaaattc agttttgggt tccaatcatt taaaagaagg
    12841 gattatgtat aaagtaggtg gatttatata ggcaaagttt tataaaagta tgtatttata
    12901 aataatttat agtctgtagg aaagaaatag ttttactata agattatgat aaaatacaca
    12961 atactatgat aaaatagtac taaataatag atttaatttt gcaaaggata aagtgtttta
    13021 ataaaaatga atgtagaaaa cgaattactt aaaaacctag atagattgca tacaacagag
    13081 ttaggcgttg taagaattaa gaaaaatctt tctttggaga caaatgatgt agtgaactgg
    13141 tgtaaaacta aaatagaatc tccaaatgcc attattaata gaaagggaaa aaactggtat
    13201 ataagtgtat atgattgtat tataacagta aacgctcaca gttatacaat tattacagct
    13261 cataaagaaa agaaatgaat tgaaaaatac aaaggtcaaa agcattttcc actgtttgta
    13321 gtacctatga agaagtatat aaaataatat tacttttgaa taataattta aattttggag
    13381 gttgaaaaaa tgaataatac tgtaaaagaa aaattggcag agtcactaac ttctgatact
    13441 actgatattt taccttattt gccttattta ttacaagatt tttgggaatt aggaagttca
    13501 ccagaacaaa tgttacaatt atttagaaag catatacctt ttaatgagga tacaaaagtt
    13561 ttggatttag cttgtggaaa aggtgctgta tctgtgaagc ttgcaaaaga gttgaatata
    13621 catatcaaag gtattgattt gatacctgaa tttattgatt atgcgagaag aaaggctatt
    13681 gaatataagg ttgatgcttt gtgtgatttt gaaatgaatg atgctaatag ttctgtaaaa
    13741 attgagcacg attatgattg tgtgattttt ggagctgtag gggatatttt aggtaattat
    13801 aaagaaacta taagtaagct attacctaca ataaaaagta aggggttcat tttaattgat
    13861 gatgcatatg tagtagataa atctacaaat gaacagctta attttgagag ggtgtatccc
    13921 acttacaatg agtggtgtat attgttcaaa gaattaggtc tgaaactaat tgaatgtata
    13981 ccatgtttag aacttgaaaa tgatgttgaa gaggaaatga actggattat aactcgtgca
    14041 aatgagctaa ttaaaaaata tccttctcaa aaagatatgt ttgaacaata tgttattagc
    14101 caaaaaaatg aatatactga tttacagcag gaccttgttg gagcaacttg gattttacaa
    14161 aaagaataag ataaatatta gactagataa tcaatgttgt agtgaattat cccatgtcta
    14221 gtgaatataa ataacataaa aaaccgtcaa gatgaatagt ttattcgtgc ttgacggttt
    14281 ttagtatttg ttgaagtaaa attttatttc tttagtatat cacttttagt caatccattt
    14341 gatttagctt gagtattcca ttgatgattc cccataagtt tatataggaa tctagggaag
    14401 ttaggtgaaa taaaaatagt acttttgttg gaatcatcta tatgtatatt atcactaagt
    14461 atattttttg aaagttcttt taatgcaatt cctaaatctt ttttaggtcc tttacccaaa
    14521 ggtacggatt ctagtgaacc aaacatctcc ccagctccaa ttccaatacc ttgaacccat
    14581 gtaaagttga tttttttgca ccaatgatct atcatttgaa ttgccaaatg attatgttca
    14641 ccttcattga atccacagtt aactattgca tacactggaa taggtttttt attttgtgtt
    14701 tttaaaaatt tttcacattt ctctaaaaaa tgaagtaaat gagatggaat accatctaca
    14761 taaagaggaa atgctattat taatctatca caattaagta tggcattaag agcgtcagga
    14821 tacttagatg tgctatctac tttaaatgta aaaactttat tatcattttg aataagttgt
    14881 tttaattcag ataaaaaata ctgtgaatta tttttattta cttttggact accatttatc
    14941 attactatat tcatatggat tcacctctta tatcctgtat attttcatag aaacaaacag
    15001 agtgttcttt tatattgaaa tttatagcat ttccatttac taatttcgta gcagtttctt
    15061 tttcagcttc tgtaatacaa tctccataga atatagcttt taaatttatc tgtttatcat
    15121 atctgctttt atgacgcatc atattatctt ttattacaaa gtatggaaga atataattaa
    15181 tgcttttatc aagtacgttt ttgacaaaag ggctaaaccc tccgtaacag catctgctta
    15241 ttataataat tccatcggat tcatttataa gtttacccat gttgctgtaa taatctttta
    15301 caacacattc agaaggagtt ttaatccagc aaccaaaacg accaatacat ttgtggattt
    15361 cttttgtgtt tggaagtatt tttatattgt catcaagctt gggtaataac ttttctaatt
    15421 ctttgtcagt taaatcatgt attaaaagct tcataaagtc catctccttt tattatgtat
    15481 gtttatttct aaaaattata tcttatagat ttttcagtaa atctttacac caattaataa
    15541 caaaattata atagtcaact ccataaatca ttgtggcata catatatgta tattggtcaa
    15601 tacaatgatt ttcttctaca tcaatttttt gtttacttaa tttatttaga agtaattcta
    15661 cttcagttat taacattttt aagtttgttt caattatttc tttgggtaga tacttgtaaa
    15721 agaatatatt aacaaggtgg tcttgctttg ttttagaaaa atttataggt ttctttaacc
    15781 attcgagaaa tacttcttta ccagtattag ttatggaata tagttttttt aatttgctac
    15841 catctataac ttcgtgatag tgtatatatc cttttgtttc taatctttta agtgcaggat
    15901 agatacttcc aaagctggcg tcaaaaaagt acgaggtact ttctgacatt atttgtttta
    15961 agtcgtatcc acttaattct ttttccatta aaaatcctaa aataatgtat tctaacatat
    16021 tacctcctgg tatcaatcag atatatatcg attcgatata tttattatta cagataataa
    16081 atattacgtc aagatgcttt ttaaaataag ctaaaaagaa ttaggaatta ttgataaata
    16141 tgcaaaaaaa ttaatataaa aggtaattaa atatcaatga agatttaaaa atagttaaat
    16201 taaagtaaga gtctaatata taaaatttat agtaacttta tggtttaaat ataaacttgt
    16261 gtgctattga caaagtataa aattagcagt aaaataattg catgagcaaa tagattattg
    16321 cgaacgcaaa aaaatgagga caatagatat taaaggagag aatatgagag agctattaaa
    16381 atgggtttcc attataaata gatacaatac aaattatatt gacaggaaat tggcggatat
    16441 tggaatcaat aatagccaat atttttatgt attacacata tgtaataatc caggtattac
    16501 acaggacact atatttgaga aaattcttgt gaatcctagt aatataacga gagctttggc
    16561 aaatttagaa aaagaaggct ttataacaag agaaccaggt gtaaaggata aaagaacatg
    16621 gcatttatat cctacagaaa aatctttaga atgttatgat gaaatactaa aaataacaaa
    16681 tgggtatgtt gaggagttgt tgagttcatt tgagaaagaa gagcaagagt tgtttatgtc
    16741 aatgttaaag aaagttgcat ttttagctat tgatatgaat gaagaagcaa aattggaggc
    16801 gaaggaaaat ggaagaaaaa tataaggagc cattggaata tgaaaaggtt tcaaagttat
    16861 taactaagta cgcaattcct agtattatag caatgttagt aagtgcttta tataacattg
    16921 ttgaccaaat atttattggg caaggtgttg gtgtattggg taatgctgct acaaatgtca
    16981 cttttccact aacaacaata tgtactgcaa ttgcattatt attaggaatt ggaggagcgt
    17041 ctaagtgttc attggaatta ggtgctaaaa attcagaaaa agctacaaaa gcagcaggaa
    17101 attctatatg cttgatggct gtttttggaa taagtttatt tatagtagta tcaatatttt
    17161 taacaccact attaaaattc tttggctcta catcagaaat actaccttat gcacacacat
    17221 atacaaaaat tacatcaatt ggattaccat ttcttataat gtccactgga atgagtaaat
    17281 tgatacttgc agatgggaga ccaaaagctt ctatgttttg tatgttaata ggagcagtta
    17341 ttaatacaat tttgaatcca ttattcattt ttgtttttaa tatgggaatt gctgggtctg
    17401 ctttagcaac tgttattggg caacttatat cttttggaat aagtgttcat tatataaaga
    17461 attttcaaca tattaaatta cataagaaaa gttttaaatt agatagtata atttataaac
    17521 atatatttgc gctaggttca agctcatgtt ttaatcaatt agcaatgact gtaacacaga
    17581 tagttatgaa taatacattg gcatattatg gggctaggtc agtttatgga agtgagattc
    17641 cacttgcttg tgtcggtatc attattaaag taaatatgat ttttatatct ataattattg
    17701 gtatatcaca aggtatgcaa ccaattatag ggtataatta tggggctgaa aagtataatc
    17761 gtgtaaaaga agcatataag tttgcagtgg gaagtgcaac tattatatct gttattgcat
    17821 tcttatgttt ccaattcttc ccaagacaaa ttacgtctat atttggtaac ggaaatgaga
    17881 cttattttat atttgcagaa cattatttta gaactttctt gttcttaaca tttataaatg
    17941 gtgttcaaat agtatcatca actttcttta catcaattgg gaaatcagtg ttgggacttt
    18001 ttacttcttt gactcgtcaa attttatttt taattccatt gatagtaatt ttaccaatgt
    18061 ttatgggaat tgatggagta atgtttgcag gaccaatagc agatgcagca gcggcaatgg
    18121 tatgtatata ctttgcagtt catgaaatga aatccttgac tataaagcaa aataatttga
    18181 tgagtaacca taataagatt gcagaggtat aagacaatat ttaagaagta tagaaaatta
    18241 atttaagaaa tataataaac taatttagga agtgtagaaa attaa
//
LOCUS       DAMJVX010000070        17910 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMJVX010000070 DAMJVX010000000
VERSION     DAMJVX010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17910
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.073"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            138..1064
                     /locus_tag="PC407_002069"
     CDS             138..1064
                     /locus_tag="PC407_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2497514.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTVLYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(1184..2182)
                     /locus_tag="PC407_002070"
     CDS             complement(1184..2182)
                     /locus_tag="PC407_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HDF2497515.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMTGKHVGRTVVDLEKI"
     gene            2286..2723
                     /locus_tag="PC407_002071"
     CDS             2286..2723
                     /locus_tag="PC407_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2497516.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            2809..3396
                     /locus_tag="PC407_002072"
     CDS             2809..3396
                     /locus_tag="PC407_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2497517.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(3696..4316)
                     /locus_tag="PC407_002073"
     CDS             complement(3696..4316)
                     /locus_tag="PC407_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HDF2497518.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(4324..5091)
                     /locus_tag="PC407_002074"
     CDS             complement(4324..5091)
                     /locus_tag="PC407_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HDF2497519.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGVIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            complement(5132..5953)
                     /locus_tag="PC407_002075"
     CDS             complement(5132..5953)
                     /locus_tag="PC407_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497520.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            complement(6310..8220)
                     /gene="phnW"
                     /locus_tag="PC407_002076"
     CDS             complement(6310..8220)
                     /gene="phnW"
                     /locus_tag="PC407_002076"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDF2497521.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNLWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(8499..9284)
                     /locus_tag="PC407_002077"
     CDS             complement(8499..9284)
                     /locus_tag="PC407_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2497522.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(9501..9731)
                     /gene="dltC"
                     /locus_tag="PC407_002078"
     CDS             complement(9501..9731)
                     /gene="dltC"
                     /locus_tag="PC407_002078"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDF2497523.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(9750..10898)
                     /gene="dltB"
                     /locus_tag="PC407_002079"
     CDS             complement(9750..10898)
                     /gene="dltB"
                     /locus_tag="PC407_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDF2497524.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKHISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(10898..12412)
                     /locus_tag="PC407_002080"
     CDS             complement(10898..12412)
                     /locus_tag="PC407_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HDF2497525.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVIFN
                     FSDERNFGDINVIDMDKLNDIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(12533..13723)
                     /gene="dltD"
                     /locus_tag="PC407_002081"
     CDS             complement(12533..13723)
                     /gene="dltD"
                     /locus_tag="PC407_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDF2497526.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGINPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(14011..15030)
                     /locus_tag="PC407_002082"
     CDS             complement(14011..15030)
                     /locus_tag="PC407_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2497527.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLHGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQAITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(15206..15745)
                     /locus_tag="PC407_002083"
     CDS             complement(15206..15745)
                     /locus_tag="PC407_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HDF2497528.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPILVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(15846..16526)
                     /locus_tag="PC407_002084"
     CDS             complement(15846..16526)
                     /locus_tag="PC407_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDF2497529.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(16555..17844)
                     /locus_tag="PC407_002085"
     CDS             complement(16555..17844)
                     /locus_tag="PC407_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2497530.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         5934 a         3130 c         1839 g         7007 t
ORIGIN      
        1 aattttttaa atcatgatgt attattttaa atatacagtt acattattat tcacaatata
       61 agtatcttag ttctcaaata acatctcatg taatctattc aatgcaaaaa atttaaatat
      121 tttttaggag gttttttatg agcaaggaaa ataaaaatgt aagaattgct gttgtccagg
      181 cgtcaccagt cataatggat ttagaaaaaa ctgttgaaaa agcattagga ttaattaaag
      241 aagctggtag aaaaggggca aatatcgtag ttttccctga agcattcata cctgcatatc
      301 caagaggtct ttcatttgga tttgttgttg gaagtagaac aatggaaggt agagaagatt
      361 ggaaaagata ctatgataat tctgtaccag ttcctagtgc tacaacagat ttattaggaa
      421 aagcagcaca agaagcagga gtatatctat caatgggtat aacagaaaga gatggtaatg
      481 atataaattg taccctttat tgcacaaact tatttttctc tccagaaggt aagctaattg
      541 gtaaacacag aaaacttaaa cctacaggaa cagaaagatg tatttgggga gaaggagatg
      601 gaagtactct tacagtagtt gatactccat atggaaaaat gggttcgttg atttgttggg
      661 aaaattatat gccattagct agaacagttc tttatgctaa gggtgtaaaa ctttacatag
      721 cccctactgc agattcaaga gaagagtggc aagcaactat gaagcatatt gctttagaag
      781 gaagatgttt tgtaattgga tgcaatcaat atgttgaaaa aaatatgtat ccaactgacc
      841 ttaactacta taaggagcta gatgcagaac cagaaattat gtgtccagga ggaagttgta
      901 tagttgaccc ttttggaaaa tatgtagcag gtcctatttt caataaagaa gaaatgctaa
      961 tagctgattt agacctagaa aaaatagtac ttagtagact agattttgat tcagaaggtc
     1021 actattcaag accagatgta tttgaattaa ttgttcatga ataatctttt tacacaaaca
     1081 tctacataaa tttttttaca taaaaaaatg agatactcat aagtaaattt caagtatctc
     1141 atttttgctt aaaacctata atcctaatta taaatttatt cgcctatatt ttttctaaat
     1201 caactactgt tcttccaaca tgttttcctg tcattatcga atctatacta tcatttaact
     1261 cttccaatga tactacattt accatatcat ctaaatcatc taatttccat tcatttgata
     1321 ataaatccca tatttttaat cttaagttca ttgggcaatc cacagaatct ataccatata
     1381 aagtaattcc ctttaagata aatggatata ctgaacttgt aaaatcaact cctcctgcat
     1441 taccacaagt tgttactgct ccacaataat tagttgtctt tattgctgtt gctagagtat
     1501 ttcctcctac tgtatctatt actccagccc atcttggtct aagcagaggt cttcctgagt
     1561 tatcatccac ttctgaccta tgtataacat ctttaacacc taagtttagt aatacttctt
     1621 tttgttcttc taattttcct gttgaaacta caactgaata acctaattta atcaatattt
     1681 tagttgcaat tacagcaact cctccagtac caccagtgac taatacatct cccatattct
     1741 tgtctacaga atctataaat ttatacacag ataaagctgc tgtaaaccct gctgtaccat
     1801 aatccattgc ttcttttagt gaaagatttt taggaagttt aactacccaa ctagaaggaa
     1861 ctcttatgta ttctgaaaaa ccaccagaag tattcattcc caagtcataa cctgttacta
     1921 aaacttcttc tccaatttta aaatcatcac aactactaga ttctactata ccagatgcat
     1981 caattcctgg tgtatgagga tactttctcg taacaccctt attaccattt gcagaaagtg
     2041 catccttgta atttagagaa gaatatttta cttttattag aacttcgcct tctggcaatg
     2101 tatctacttc tctttcaact atatttcttc taaaatttcc ttcttcttct gtaatatata
     2161 gtgatttaaa ttttttattc ataaatttcc tcccaataca tttagtttta tgttaaacta
     2221 attatagttt ataaatagtt ttatgtcaaa ctaatttaaa tagattaaag attggagaat
     2281 aatttatgga agataaatat attgtacatt tcataagtaa aacaaaggct agtatgatta
     2341 aattcattga aaataaactc agtaaaaatg gtttaggtga gttaatacct actcatggaa
     2401 atattttaac tgcactttat gaaaataatg gaatacttac aatgaaagaa attgcagcta
     2461 aaataggaaa agataaatcc actgttacag tgttggttaa taaacttata aacctaggat
     2521 atttagaaag acaaaaatgt acaaatgaca aacgtatcac gtatataaag cttacaaaaa
     2581 aggcactgct tatagaagat acttttaata gcatctcaac acaagttaaa gagacagcat
     2641 accataatat tacagaaaat gaaaaacaag aatttcttag aattttaaaa aaaattaatg
     2701 ataattttaa aaatgctgac taaattgagc ataattatat ctcctattgg aataataaat
     2761 aaaaagaatc aaatattgta aaaatttatt atgatagaga ggtagaatat gaatttaaaa
     2821 gatagtaaaa ctaaagaaaa tttattaaga gcttttgcag gtgagagtca agctagaaat
     2881 agatacaata tttctgcatc tgtagcaaaa aaagaaggat tatatataat agagtcttta
     2941 tttaattaca ctgctgacca agaaaaatca catgcaaaac aattcttttc taaattaaaa
     3001 gaatttgctg gtgaaaatat aagtatatca ggagcatacc ctgtagattt atatgacaag
     3061 acagcacaac accttaaagc tgcacaacat aatgagcttc aagagtggga tgatgtatat
     3121 aaaaactttg gaaaaacagc acgtgaagaa ggatttgaat ctatagctaa gttatttgaa
     3181 aatatagctg aaatagaaaa agttcatggt gatagatttg gtagatatgc tactgattta
     3241 gaagaaggta ctttattcag aagaaatgaa gatatacaat ggatatgtac acattgtggc
     3301 catatacata taggtaaaga agctccaaaa gcttgtcctg tatgtcttca tccacaagga
     3361 tatttcttag attttaaaaa ctcattattt gaataaacta aggcaataat tctatttaaa
     3421 caatttacta tatttatatc aagtattttt ataaatactg tattatctta ataacaataa
     3481 aatgaaccat tgatattact ataagtagaa aatgtagttt aaatacaatt tatagtaatt
     3541 catcatggtt cattttatta tttactaatc cctataacct acttaaatct atctttaact
     3601 tttacatact cacataaaat cttatcttta aactaaatat ttaaataaag tgtattttaa
     3661 gtagattatt ataatacatt atataagata atacactatg ataactttat acttatatca
     3721 tttatttctt ttgtcaagtc aatctcaata atcttaacta catctttttc aaactttact
     3781 tttttataat ccagacatcc tactacgaat ggaactccaa actttctaaa caaataaatc
     3841 tttccacttt cattgtatat tgatgtaaaa ttttcttcta ttatattcac tatcaatttt
     3901 aactcttcaa aagttattat agatttaact aaaaacttct ctattatgct caatataagc
     3961 agttcttttt tacctatgct ttgctctttt ttatataaat tttgaaatat ttctttggta
     4021 taattacata taacttcttt actgagaata aaatcaatgt caaattcctt attaatatcc
     4081 tttttcataa acatatttgc aatatcatca agagaagtct cttctttcat agacaatatt
     4141 aactctattc tttctagtat tttatctctt ggaaagaaag tctcttgacc tgtaaaagct
     4201 gattttttta taaaccattc ctctggtatt atgttttttc ttttccatct atataattga
     4261 ccataagata tattagtttt gctcaataaa tctttcttag atattaattc ttccataact
     4321 tttctagccc acctttatac tgttttttac agtagaatta tctgaaactg ataaattatc
     4381 actgtattca atatttccaa cgtcacaatt atctactaga gatatgcttt tgcctcgtat
     4441 atcttttatt gatgcatctt ctaattcaat agtatctccc tctatcaaat tacatttaaa
     4501 ctttcctgaa aaaatattaa tgattttaat taatgaaaaa gcatctttgc ttcttcctat
     4561 ttttatatta gttgccccta tctctttaca ataagaacct cctcctgaat aaacatatat
     4621 attttctcca tttaaaagac catcacattt tattactcca tataccttta tatcttcaca
     4681 ttctatattt gatttatttc tcatttcgcc atagattttt atatcttgtg catacatatt
     4741 ttcatttaca cttaattgtc ctcttattgt aacattatta catttaagtt tgccatccac
     4801 tttctgttga gcagttataa ctaagtcatc tatttctgca tctccctcta tattaagttg
     4861 accattcact cttacacgct ctgctttaag attgcctctt ataatagttt ttccgttaac
     4921 aacaaaattt tttatattga tgtcaccttc aaattttgca tctccaagga cttttacttc
     4981 gttactttta acatttcctt ttgatttaga acttcccacg atttttatat tttcatactc
     5041 accactactt ataaagcctt ctccaagtat ttttatagac cctttttcca tattattacc
     5101 tcattttaaa ttttattttt aaacctattt attacttatc cttctttaat tttatttttt
     5161 cacttatatt ctttatttca ccaactattg aatcttcatc aatctctata ctttcactgt
     5221 actccaaact ttctacataa catcccttgc ctattttaat attttttcct cttacaactt
     5281 tgacttcact attttctaag cttatttcat ctgcttctat aatatttgca aacaatttac
     5341 tttctgtata ctttctaatt gataatagac ctaaacttat taaatttgaa gacacttttt
     5401 caaagttgat atgctttttt tctagcatac gaaatttttt gcaataatct ccatgcccct
     5461 tttttatatt tatactactt gctccaactt cattaaaatt agaagttcca tttaaactaa
     5521 tattaacttc ttcacaattc aaaaatccat tacactccaa gattccactt aaattgatat
     5581 tttcacaatc tatattctca tttgctttta aatatccttc tccatgaata atatccgatt
     5641 ttatacttcc ttctacatta ataattccac tagtatcaat ttcaacagaa ttaatatttc
     5701 ctgtacatct aagaagtccc gatatctcaa cttttgatgc cagtatgttt ccttctactt
     5761 tacttaaacc agatgtaaaa aattcattac attctgttgt cccaaaaact tttgctgaac
     5821 cagaagaatc aaatttacta cattttacac ttccttctat tttgcctgtt cctgatatat
     5881 taacattatc aaaaattcca cctttacatt ttccaacacc atcaaatctt agtgaactta
     5941 attcttcatt cataatgata tcctttcttt accgtatttt atctttaaac ctaatataac
     6001 aaaacattgt tacgttgtca agtctgataa taatacttaa taaagcaact atcatttatt
     6061 ttctataaat aggcataaaa ataataggga aatgctttat ttaaactaga aatctaattt
     6121 cttagattgt ggtttaaata aaatatatcc ctattactca atctttatat attcattgta
     6181 taaacttcta aaatataagt ctttaaattc tactgtcaat actttttata cactgtttgt
     6241 gctatcattt cacacctaaa ggagtaggct ctccgcacta ttctgtaacc taaaagacac
     6301 tatcttcttc tatctattga taaatttttc tattacatca gctagtctct ccatatcagt
     6361 tggataaact tcacctatgc tacctattct aaatgtatct atatcagtca atttacctgg
     6421 atagataaca aatccattat cttttaaata agtataaaat tccataaatt caaactttgc
     6481 atttttagga tataagaaag ttgttattac aggtgattga gcattatcat tcaccaaagt
     6541 atcaaatcct aaagatttta atcttgaagc tattgtaaac tgattttctt tatatctagc
     6601 atatcttttt tcaacagaac cttcttcttc caattctaat aaagcttgat aaaaagctct
     6661 tacaacatgt gtaggtgaag taaatctcca tttaccatta tttttctcca tagtctccca
     6721 ttgcgcatac acatcaagag ataatgattt agctatacct ttacatttag ataatttctc
     6781 tttattagct attataaaac caaatccagg tactccttgt atacatttgt tagaactact
     6841 aactaaaaaa tctatattaa aatcttcaac atcaatctca attcccccaa aactactcat
     6901 agcatcaact atataaattt tgttatattc ttttgcaagt ttccctacct cttgtatagg
     6961 atttaatcta cctgtagtag tttcacagtg aaccattgat atatgtgtta tatccttgtt
     7021 ttcttttaat aaattttcta ctacatttaa atccactgct tctatatctt taaatgtgca
     7081 gtcaacaaat tctatgttaa gataattaca tatatctttc attctctttc catatgctcc
     7141 atttgcaatt acaagaagtt taccatcttt tgaaatagta cttcctatta tagcttctac
     7201 tgaaaaagtc ccacttcctt gcattaatac acttgtgtat ttatctgtat tttgggttgc
     7261 tagagatact aatcttcttc taacatcttg tactaggtta ttatattcaa catcccaagt
     7321 acaccaatct tttaacatag atgctcttac tgatttagta gtagatagtg gtcctggtgt
     7381 aagtaatatg taatcatttt ctggtacaaa atctgattta atagtttcat tctctatttt
     7441 tattagtata tcttctaact cagccatagt ttctattaca aaatgtgcac ctgcctcttt
     7501 aaatttcttt gaaactatac tcatcttagc ttttaattct tccttatcca tgttctctac
     7561 ttcttcttgt gtaagcccta actcacttga accttttatt acagcaacac tccacattcc
     7621 tgcatttacg ccttctttta catcacttat tgtatctcct acttttacca tagaactcat
     7681 aggagacacc cctaaagctt cagcattttt atagcacatc caaggatacg gtcttccttg
     7741 agatacttct gatggtgtta caagaaaatc gggtgagtat cctttctttg ctgcatttgg
     7801 ttcaactatg ttcatcatct ctcttgtata acccgttgta gaacctattt ttaatccatt
     7861 ttttctcaac ttttcaatag tttcaacaac atgaggaatt ggagtcgtat actcttctaa
     7921 tgtctcaaat aacattggct caaattcagc ataaagttca tttacatcat cttcagttgg
     7981 cactttacca aacttatctg accaaagatt ttttattcta tccatttcac acatttctct
     8041 tatatggtct atttttaatt ttcccatagg ttttcttgct tcttccattg ttacatctat
     8101 acctcttctt ttaaatatct ctataaatac attaagaggt gcaaaacatc cataatctac
     8161 tgttgttcca gcccaatcga atactactgc ttttattttt tctccatata tctttttcat
     8221 atttatagcc tccatagatt cattttagaa tttttgtgtt tttaattgtt tttactttta
     8281 aagtataact ttcatatgtt aattatacat catatttagg ttaaatatat attaaatttc
     8341 tgtttttagt tgtatttaat tgtttttatt ttatatcata tttccataaa atcctaagtt
     8401 acaatctaaa atttactttc taatcttaat ctatgaactg actatatttt atgaatttag
     8461 ttgtattaag catagttgta ttatataaaa ctaagggttt attttgcttc tatccatgtt
     8521 atatttatct catttaactt atccttccat tcattcggag gttctacatc acaaataaca
     8581 aaatctaatt cctctaaatc tgctatctta tacatactag caattcctat tttagaacta
     8641 tcacataata caaaactttc ctttgaattt tttatatatt cttttgacaa caatgcctta
     8701 ttttggtcta tactactaat tccaaaacta tcaagtatac cttcactaga tataaaagct
     8761 ttatctacgt ataagttttt catagtgtct attgacattg gacatgctgt cctttggtgt
     8821 tttaaattta cttttccacc aataaatata acttctccat caaacaaacc ttgattctca
     8881 taagaaatca attttgatag aactggataa gagtttgtaa taatttttaa attgtttaca
     8941 gatattatat aatctacaat ctgcaaattg gtagtacctt catctatgat aattgactca
     9001 ttgtcattta caaacttagc tgcgtattta gcaatacttt gttttttatc taggttagta
     9061 ttgtttctct ttatatgttc tggttcaatc actccataat ttatttttat agcaccacca
     9121 tatacctttt ttaatttatt ttcattttct aattcttcca aatatcttct tattgtttct
     9181 gatgatactt ctaatctttt tactaattct ttagtattta ccttcccttt tgaatctaat
     9241 aatttaagta tttctttttt tctatctatc gaatttaaag acattcattt tcctcctaaa
     9301 tataatatat ttttctccta taatattaca atgataccta agatttatga aatcatattt
     9361 atgtattaaa ttttttactt aattaaagtt attttcaaaa gactttgtat tatcattaaa
     9421 aaagtccatc aaatattagt atcagatgga ctttgtaaaa ttttatttta taaattaata
     9481 tttaaagtct aactactaat ttattttctt gtctccaaga attttactaa attatttaca
     9541 gtagacatat cttttctctc taaatcagtt ggttgaagct ctatccctaa tctattttca
     9601 atttcaagta aaacttcaat tatagctaat gagtctaaaa gttccgtctc aaataaatta
     9661 aggtctaaat cttctcttat ctcatcagtc cctaatacat cttcaaatat ttctataact
     9721 gtttcttgca taaattttcc tccttaaaat taagctaaat aacctgagaa tatcagcaat
     9781 ccaaaacaca caatatggaa tgtaatgaaa atctgcactc tctcgaacca tttatctttt
     9841 ttatgttttt tataaaattt agattttcgc tgatatatat cagttaacac aagtgccaat
     9901 ccttggtata caccataagc aacataatac caagttagac catgccaaaa tcccattgtt
     9961 atcatggtta tcatctgagc cacatgagca gcagtagtcc ttttcttaaa tcttttcttt
    10021 ctcattgatg acataacaaa tctagaaaaa atataatctc caaaccatct tgaaagactt
    10081 atatgccatc ttgtccagaa ctctttcata tctttactga taaatggctt gtcaaagttt
    10141 ataggtactt gtattccaaa tatgtaccct gtacctatag caaataaact atatcctgca
    10201 aaatcaaaga acaggtataa actatatgcg tacatataac ttaaaatatt tataaaactc
    10261 atatctttag gtattcttga tacccaatat gtatttatca aaaatgctat aacaaactta
    10321 taacctactc ccatgacaat attttttatt cctggcagta agtattcttc tatatactct
    10381 tttctagaga tgtgtttttc caagtcctgt tcaaatcttc ttgacctatc aataggtcca
    10441 gaacttaaag ttggaaaaaa taacataaaa taaagcatct ttgaaatttt aacttctttt
    10501 atagctccat cataaatttc aataaccatt tgtatagttc tgaaatttaa gtatgatatt
    10561 ccaataaatc caattattcc aaaagaagta actggagata ttttatttat tattattggg
    10621 agcattgatg caaacaagaa tccccaatat atgtatttat ttttagtttt tcttcttaca
    10681 tattcatatc ctttaactat tattacttca agaataataa atattaccaa atactttagc
    10741 tggacatcaa ttcctactat taagaatatc ataaacaaac ttgccaacat accataatac
    10801 tttatattct tgcccattag acctaaaatt actgctggta ttgatgttaa taataatata
    10861 taaagataaa aataatctcc atactgtgaa aaaatcatta aatttcctcc ataagtttct
    10921 ttctgtctat tttaccattg atatttgttg gaaactcaga gattattgaa atatttcttg
    10981 gaatcatata tgaaggtata tactttccaa gttccttttt tattatcatc ccatttttaa
    11041 tattacttaa atcattcttt tcatttaact caacaatacc ttttaagtaa gcaatttttt
    11101 cgtccttata tactggtaaa actactgcat tttttacatt atgtactttt ctcaaattat
    11161 tttctatatc ttctatttca attctaaatc catttaattt aatctgaaaa tcttttcttc
    11221 cacaataata tatatttcca tctaaaagat atcccatatc tccagtttta taagctctcc
    11281 actttacacc atctatttca tcatagaaaa atacttcatc agtcttttct ttattattga
    11341 agtaaccttt agaaactgat ggtccaatta ttataatttc acccttttca ttttctttaa
    11401 gttcatttcc atcttcatca agtattttta ttttacaatt tgacataggg tatccaactg
    11461 gaagactttt ttcatcatct atagcttttt gagtcatatc atttacactt acaccaacag
    11521 tagcctcggt tggtccatat ccattgataa ttcttgtgtt tggaaatcta ctcataagtt
    11581 ctttagtaag attttttgaa agtgcttctc ctatgaatat cattgactca aggttaggaa
    11641 gcattttact attaaactct ttttcagtta tacatactcc tgcaaaagaa ggagttgaca
    11701 cccatacagc tatatctgag atactaaatt gtctaaatag ttctttatag tctgctaata
    11761 catcttttga tattgaaaat aatgtagctc catggataag accagggtat attgttgtaa
    11821 ctgaaaggtc aaaagaatat gctggttggt tcattattac tttttcactt ccatctatgt
    11881 ttagatatgg gcttatccaa tctgaaaagc tatctaaatt gtttgaactt atttgaaccc
    11941 cttttggctt tcctgtactt cctgaagtaa atagtatata tgcattttca tcatctttta
    12001 cccagttttc tttatctaat gattttccct gatattcatt tattatatca tttaatttat
    12061 ccatatcaat tacattgata tctccaaaat ttctttcatc actaaaatta aatattactt
    12121 ttggttttat ctctttagtg acttcaaaaa ctctatcaat tggaaaacta atatcaagag
    12181 gcacatacgc tctacctgat tttaaagctc ctatcataca tgccattatc atgttttctt
    12241 tatttccata aattactata ggagtatcct cttctttgta tacatccttt agaaatactg
    12301 atatagcatc tgaatattca tttaaatctt tataagaaag tttatctcca ttacacatta
    12361 gtgctgttct atctgtattt gaatattttt ttattccttc aataattttc atataagaat
    12421 gctcctttaa aatccgttat aaataaatgg caattcttta aatggagtaa atgttagaac
    12481 tcctatcgcc aatattataa ccatagttaa acaaatacta gttaacaaat ctttattgtt
    12541 ctttaaatat tttaaataat ctctcacaga catctaccca tcctttcgta ccaagatgca
    12601 ttacatccct taaataatac ttatctgacc ctttatcttt aaggtttaga acttcaaagc
    12661 ctttgctttc tgctattttt tgtgctttat cgtagtactg atttctttct ttttcagaaa
    12721 taccagtcaa attatagaat ttatccatac ttgggattaa tacaacaact ggattaatac
    12781 ctaagtcagt acatacattt agcatcagtt tataattttc aaactcctta gatgttaata
    12841 aatttacatc tttgtctctt cctttgtatt gctctatacc atctttaaaa tgctctttat
    12901 aatagtactt atcaatattt agtggatttt taccaactct ttttttagca tcttcaatgg
    12961 ctttctttct ctcatatgac caatttattg gtctttttct ttttgttgct ctctttttat
    13021 ctagcttaat taatcgttta taaagaatac ccttttcttt aagagctata cagtactttc
    13081 ttccttgaaa ataaggctct aaaagcacct tttcagcttt ttcaggaaat gtcttaggtt
    13141 catatagctt agcatataat gcttctgctt tatactcatc acttccccat aatagcttac
    13201 ttgatttttt tgcatattca atcttatttt gtttagatat ttttggatta tctaaaaatc
    13261 gataaaattg tattggtgaa aatctagact gataatggtg acttgtgact ccgtcttttt
    13321 ccataaacca ttgcatagaa attaacaaga caactttctt attatcaata ttaggattca
    13381 tacttcctaa tattgcagca tcttgtaatg tctgagtata ggctctccca attgcaaaca
    13441 ccttattttt actcctattt gtattaaaat agtactttgg atgttgtttt gttgaattac
    13501 taagttctga tgaccccaaa atcataatat ccttttctct taaaaaatga ttatttgctg
    13561 taaccccttt atcttttata tcacttaaag tatcatccat aattggaagt agatttttct
    13621 ccctcaaaag ttcatcagtt ttagaatcaa gaaatttgtc taacccaaat aaaaatacta
    13681 cgcctattat aaaaggtgta ataaagtata tcaattttct catattttct ctcctctaaa
    13741 aatattcaaa tttatgttat tattaatcat attagttttg atatgtatat tcaactacaa
    13801 aattgtaata aaatcgtcaa aacttttttt gatactgtta caaaattgta aaaaaaaaga
    13861 aaaaaacacc aaaaataaat ttctttttag cactttttca tacttataat ttgacatttt
    13921 aacacttggc taagtataca ccttacctta acttaaaatt aaatttattt aagaatatat
    13981 attataaatt cttatatttt ctaatataag ctatatacta tcagctggat aaacaatact
    14041 actttgtctt cttatctcat ccataatttc cattactatt attgaatttt catgagaatt
    14101 tatatttgat tctattttat tgttatttat aagacttata aactctacaa gttcataata
    14161 cattccttct atttctctag gcttttcctc tgctttttct tttggaatag atatatcttc
    14221 ttctcttcca tctctgtatt taataatagc tttttcaaat agattaagct tttcaattat
    14281 tatacttccc tcttctcctt gtatttcaga tggtatataa gaatcagcta ctttagaata
    14341 ctgaattatt gcatccatat catcatattg aaatacagct gaaccttctc catccactcc
    14401 agtttgaagt atatgagaaa ctgcctttac agtttttgga gcaccaaata aattcaccat
    14461 aggatgtata caatacactc ctatatccat taaagctcca tttgacaact cttttttaaa
    14521 ggcattttct ataattccat ttttatattt atcatatctt gaagaatact gacaatagct
    14581 tccaaaaaat cttcttattt tccctatttt atacaagttt tctttaactg ctttaaagtt
    14641 tggaacacat gtaattctca tagcttccat aagcaataca ttattatctt tggctgtttt
    14701 tatcattgat ttgacttctt ttaaattaga tgctaacgac ttttcacaaa gcacatgttt
    14761 tttgtgtttt aaacaagtaa tggcttgctt tgaatgtaat gcatttggag aagctatata
    14821 tacagcatct atatccttcg atttagccat ttgttctaaa tcatcaaaaa aaatacttgc
    14881 tccatgtaga gttccaaatt ctctagcttt ttctaaattt ctagaatata cagcaaccaa
    14941 ttcaaaatct ttaaccctag atgcagctct tagaaatata tttgctatat tgcttgtccc
    15001 aacaatacca aatcttattt tacccatcat aaatcactcc ccattttcta cactttattt
    15061 taattttaat aaaaaacttt acaacttata gatatattat aaaataattt tatcaaataa
    15121 aaaagaaagc taattttaaa tgatttcacc attttaacat agctttcttt taactacaat
    15181 ctaaattcaa cttatatttt taaagttatc tttctactat atctttattt cccctcaaaa
    15241 attttatagt aagaatttca tccacaaaca atgttactaa aagtatagtt acaagaattg
    15301 gccaataagg tattataaga ctatataaac tatcaactat atacacttga aaatatctaa
    15361 ctccaataaa tgtcaatata gtccaatata cagaaaactt tagacatact attcctctac
    15421 tattgtactt ataatctgaa taatcccaat agaccttatt aaaatatact ttcataagta
    15481 ttcctgtaat atattctact actgtcggca ctactagaca taataaaagc aatgcaaata
    15541 tattattggg gtcaataaat cttgagacct ctattataat cgacattgca attgcataca
    15601 ttggtttaaa tggaccattt aaaaatccat cttcttgaaa atgcccttgt ttataataac
    15661 tatataagtt ttcaattatc caacctataa aaccatacaa gaaaaaatta aataaaaaat
    15721 ataatagaga acctacatta tccatgaata ttcctcctag ctgtttatac ttattttttc
    15781 tcatattcat ggatttattc taaatcaaag aaattatatt taatttattt aattaatact
    15841 ttatattata gattgtgaca ttctttagag ttcaaacttt ttaggtattc tctaacttcc
    15901 agtaaatttt caagtatctt atgtgattgt gatttaattt gctcatctgg ctcttttaaa
    15961 tctggtacat ttatacatct tatcccacca ttgtatgcag cttcaactcc cataggcgaa
    16021 tcctctatga caatacaatt ttggggatta acatttatct ttttagctgc ttttaagaat
    16081 atctcagggt ttggtttaga gtttacaaca tcatctccac aaactatagc atcaaagtaa
    16141 tcttttaaat ttgccttagc taatctcttt actgctcttt ctctctttgt tgatgttgca
    16201 acagccattt tgtatccatt ttcctttaaa aaagagatta gttcattgac tcctaatttg
    16261 ataggagctc ccttcctctc cataaattca atcatatttt tagttttttc gtcatataaa
    16321 tctattattg gtacagaact atcgtatata tctgtaagac cttctattat tcctttacga
    16381 tttcttccca taactgatgt ataaatttcc ttagtcatag tatatccata cttttcaaac
    16441 gtctccatcc aaaattcaag agaaattctc tctgagtcaa acaaaactcc atccatgtca
    16501 aaaataattc cttctacttt ttgcatacaa ttcctccaat attttttgat ataattatat
    16561 tacacttttt atgattaatt ttacaaccct caatttatcc aattctaata ttgtaaaatt
    16621 gtaattttca aaagttacgc tttggtttac tttaatattt gaaccaagca tataggatat
    16681 ccacccacct atactgtcaa atccatcatg ctctatatca agtccaaaat acttattaat
    16741 atagttgata gattcagttc catcaactaa gtaagaacct gctcctattt tcttaatatg
    16801 agtctcttct ttgtcaaatt catcttgaat ttctccaact atctcttcta gtatatcttc
    16861 tattgttacc acaccagatg taccaccata ttcatccaca actatagcaa gttgaagttt
    16921 ttcctttctt attctctcaa gagctttatc tatagttaaa ttttcagata tatatataat
    16981 atctctcaat atttcttcta gttcaatttt attttcattt atcttttgat tgtataaatc
    17041 tcttatatgt acaaaaccta atatatcatc tttatttttt ctgcatactg gatatcttgt
    17101 aacaccttcc tcttttaaaa tagctaatat cttttcttca gaatcacttt catatataca
    17161 taccatatca gttctaggaa ccattatctc tctaattttc ttttcttcaa attcaaaaat
    17221 attatcaact aatctttgct ctgattcatc tataagtcca cttttataac tttcttctac
    17281 taatagtctt atttcgtcac ctgtatgggg ttcatcaacc tcatcagctt gtgaatatcc
    17341 aaatggttta agaaataaat cagtacttaa gttgaaaaaa tatattattg gatatgttaa
    17401 cttataaaat cctactaata aaaaagatgt actcaacatt attttttctg tattataaag
    17461 tgctaaagct ttaggaacca actctcctat tacaacctcc atcattgtta taattaaaaa
    17521 agaaatggaa attgatatag tatatataat actttcacta agattaaata cacttactat
    17581 tggtaaaatt aactctttaa ctgtagattc tcccatccaa cctaatgcaa gtgaacaaag
    17641 tgttatacca agttgacatg cagaaagata ggaatttaag ttgtctttta ttattttaca
    17701 tctttttgca ttattattac cttcagttat catggtatca atcctagaac tacgaagttt
    17761 taccatagca aattcacaag ctacaaaaaa agcatttatc aaaacaagca ttactataag
    17821 tgttactttt aataactccc gcataaagta ttggttctta taatgtaaga aatctataag
    17881 aactcaaagt tcccccctta tatttataaa
//
LOCUS       DAMJVX010000071        17532 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMJVX010000071 DAMJVX010000000
VERSION     DAMJVX010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17532
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.074"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            229..1158
                     /gene="glsA"
                     /locus_tag="PC407_002086"
     CDS             229..1158
                     /gene="glsA"
                     /locus_tag="PC407_002086"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDF2497531.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            1256..1819
                     /locus_tag="PC407_002087"
     CDS             1256..1819
                     /locus_tag="PC407_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2497532.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            2307..3143
                     /locus_tag="PC407_002088"
     CDS             2307..3143
                     /locus_tag="PC407_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDF2497533.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHIGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            3395..4390
                     /locus_tag="PC407_002089"
     CDS             3395..4390
                     /locus_tag="PC407_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2497534.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRNKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(4647..5051)
                     /locus_tag="PC407_002090"
     CDS             complement(4647..5051)
                     /locus_tag="PC407_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF2497535.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     FLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            6051..6965
                     /gene="queG"
                     /locus_tag="PC407_002091"
     CDS             6051..6965
                     /gene="queG"
                     /locus_tag="PC407_002091"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDF2497536.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFVDNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEMPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            7071..9308
                     /locus_tag="PC407_002092"
     CDS             7071..9308
                     /locus_tag="PC407_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2497537.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNRLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDKSDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNESSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            9505..10557
                     /gene="nth"
                     /locus_tag="PC407_002093"
     CDS             9505..10557
                     /gene="nth"
                     /locus_tag="PC407_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDF2497538.1"
                     /translation="MKNVNLKEINEKDVIVEEDINLKGMDKKDVIEKEEVNKKSIDKK
                     DIIEKEEIDKKNVKKVEVQKEIDKMISKKDEIIDEKDIENFRYIEEDKKSIKVSKKST
                     KKGETEKKTKKSSSNKATQKQSKEVKSKSKIKSESANKKDVNKILDELEKLYPDAKCE
                     LNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGL
                     YKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDT
                     HVFRVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASC
                     PIKEDCNYYKELNETK"
     gene            10751..11221
                     /gene="trmL"
                     /locus_tag="PC407_002094"
     CDS             10751..11221
                     /gene="trmL"
                     /locus_tag="PC407_002094"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDF2497539.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            11257..12105
                     /locus_tag="PC407_002095"
     CDS             11257..12105
                     /locus_tag="PC407_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2497540.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVDEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            12444..13448
                     /locus_tag="PC407_002096"
     CDS             12444..13448
                     /locus_tag="PC407_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2497541.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNAALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGIKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            13573..14685
                     /locus_tag="PC407_002097"
     CDS             13573..14685
                     /locus_tag="PC407_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2497542.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSVPTSAEKDKLNSKSKNEEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            15108..15869
                     /locus_tag="PC407_002098"
     CDS             15108..15869
                     /locus_tag="PC407_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497543.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            15872..16690
                     /locus_tag="PC407_002099"
     CDS             15872..16690
                     /locus_tag="PC407_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HDF2497544.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            16870..17472
                     /locus_tag="PC407_002100"
     CDS             16870..17472
                     /locus_tag="PC407_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HDF2497545.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
BASE COUNT         7243 a         1607 c         3233 g         5449 t
ORIGIN      
        1 tgttaggtta gaaatttttg acgttataag aatttaaatt actggattat tagattacta
       61 aattactaga ttattaaatt attaagctat ttaaactgtt taaattgttt gaaaaatgaa
      121 ttgattcttg tagaaaatag ttttaaagta taattttttt taacactgtt ttattatagt
      181 acaaacttgt gctaaaatag aatatataac ataatataag aggtgcatat gaatacgtta
      241 gatgagacat tattaaaaga aattataagt tctaataaga agtatactaa ttatgggcaa
      301 gtcgctagtt acataccaga attaaaaaac gctagaagaa atgatttagg aatttgtatc
      361 atagatagcg aaaataatct ttatagtgct ggaaactgta gcacaaagtt tactatacaa
      421 agtatttcaa aaccaattgt gcttgctatg gcacttatgg ataatgattg ggaagacgtt
      481 ttttcaaacg ttggaatgga gccaagtggg gacccattta attcaataat gaaacttgaa
      541 attaatgaca caaaaaaacc ctgtaatcca atgataaatg caggggctat agtaactact
      601 tcccttataa atgggagttg tttagaagaa aaagaagaaa gaatgcttag tttctttaga
      661 aagttagcaa aaaatgataa tataggaata aactatgatg tctataagtc tgaaaaaatg
      721 actggagata gaaacagagc tatggcttac ttattgaaaa gtgatggatt tatcagaggg
      781 aatgtagaag atgttctaga cctttatttt aaacaatgct caattgagat agattctgta
      841 gatttagcga gaattggaat aaatcttgca aattatggag tagatataga aaatggtgag
      901 catttaatga gtgaaatggt ttctaggatt gtcaagacat ttatgatgac ttgtggaatg
      961 tatgatgctt ctggggaatt tgctattaaa gttggcattc ctgcaaaatc tggagttggg
     1021 ggtggtataa tggcttctgt gccgggacgc atggggattg gtgtttatgg acctgcgctt
     1081 gacaaaaaag gtaacagcgt cgcaggagtt aaagttttag aggagttgtc aaataaactc
     1141 aaattaaata ttttttaaaa tattaagaaa ggattttttt gacaacaaca tataatttaa
     1201 tatataatta aattgtaaag aaatatgaat taaataaatt ttttaggagg ggtcaatgaa
     1261 agaggtattg gtattacgag atttggagtg cataaaggct attgcgcatc cacgtagaat
     1321 agatattcta aaagcatttg acaaatcgcc attgtcagca aaacaattat ctcaattatt
     1381 ggaagaacct catgcaaaaa taaactacca tataaaaatg ttatacaaag ttggtatttt
     1441 agaattagtt gaggaaaaaa taaagtcggg gattgtggaa aagtactatt atccaagtgc
     1501 aaaaaatgtg gttataggga ataggatact taatttttct ttagacaatg gggaagaaaa
     1561 agaagaattg tacatatcaa aatttgaaaa tatgagtgaa gttttttata aggcagcaga
     1621 ggaagatgtt ttagaaaatg aaaatatagt tgattatcat gatatttctc ttactcatga
     1681 tgaattagtt gaacttagtg acactatgaa gtcaaagata gatgaaattt taaataaaag
     1741 acaacacaat gttgaaggtt ctaagtatga tatagctatg gttatagttc ctactcttga
     1801 ggaggaatgt cctagttaga gaattaatta tggattaatt aaagatataa tattagagat
     1861 tgaacatcaa aatttactta ataatcaaaa aatagttttt ggtatgggta aaacaatgat
     1921 ttttaatctc tatttttttg ttttcttata cctattgtat ttgtaaataa tatgtatagt
     1981 gtaagatgta tacactatct gagaagtgct tagataatat acaaagtatg taagattata
     2041 atatttgaaa ttgattaaaa tgggtataat taaagtgtaa atattaaaat taaaaattaa
     2101 tataaattaa atcaatgcta gaaatatgtt aaaagaggaa tgagattagt tgggatagaa
     2161 catcccttag agcttatgaa tttttagtga aaaagtacta tcaaatagga atctccatct
     2221 tcatatgttg gctaaatata gttaaggtgg agtagttcaa aattaaaatt gagtacaagt
     2281 cgtagtacaa ataaaaggag attaatatga ttaaattagg aacacatctt tcaatagcaa
     2341 aaggatttgc taatgcggct gagacagctg tatatattgg tgcaaataca tttcaatttt
     2401 ttagtagaaa tccaagagga ggaaatgcta aagaatttga tgaaaaagat ataattaaat
     2461 ttcaagaaat tagaaaagaa aataattttg caccactgct tgcacatgca ccatatacta
     2521 tgaatctggg tggtacaaag gatgatgtat acgaatttgc aacaagggtg atagatgagg
     2581 atataagaag gatggactca ttaggaattg aatacatgtg ttttcatcct ggaagtcata
     2641 taggtggtgg tgtagatttt ggtatagata gaatagtaaa aggattgaac aatgccataa
     2701 agggtgatga aaatataact atacttcttg aaactatgtc gggaaaggga actgaaatag
     2761 gttttaaatt tgagcaatta aaaagtataa tagacaaaat tgagcataaa gaaaaaatag
     2821 gtgtgtgtct tgatacatgt catatttttt cagccggtta tgatatagtg aatgatttag
     2881 atggggtttt ggaagaattt gataaggtta taggcataga aagattgaaa actgtccatc
     2941 taaatgatag tatgatgcca tttggagaca aaaaagacag acatgctcct ataggggaag
     3001 gaaagatagg attaaaagca ttaattgatt ttatggaaca tcctagtcta aaacatttac
     3061 catttttctt agaaactcct tttgatgatg aaggtcacaa gagagagctt aaaatgataa
     3121 aagaaatact atctagtaaa taaaccttta ttaaaaatat gtgaaatact ccgccctcaa
     3181 cataattaag gtggagatac aatcatgaaa tgtgataatt taaattaatg gataggaata
     3241 acaaaaattg tatacaaatt aactgggtat aaagtatgat tatattatga atagtatgta
     3301 aatatattgc aaagattctc ataatttgtt gtataattaa aggtaacata tttatattat
     3361 ataaaaaatt attaaaagtt aggtggggaa agatatgaga aatttaggca atactaacat
     3421 gaaaataaag agagttggct ttgggggcat tccaatacaa agaattactc aagatgatac
     3481 taacttagtt ataaatgagt tagaaaaaca aggaataaac tttattgaca gtgcaagagg
     3541 gtatacaata agtgaggaag caatcggaat tgccattgaa ggaaaaagaa ataaattttt
     3601 cttagctact aaaagtatgt ccagagatta tgattcaatg aagagagatg ttgaaataag
     3661 tttaaataat tttaaaactg attttataga tttatatcaa ttccataatg ttaaagaaga
     3721 ggagtatgat aatcttttta aagataaaat ggcttatagt gcccttttag aggcaaagga
     3781 gcaaggtaag ataaaacata ttggtataac tagtcacaat ttaaatacta tagaaaaagc
     3841 tatagaagat ggaaaatttg acactataca attcccttac aatatagttg aaggacaagc
     3901 agatgaagtg tttaaaaaag ctcatgaaaa aggaattggg ataatagtaa tgaaaccttt
     3961 agctggaggt gctttagaca atgcaacact ggctattaaa tatatactat ctaaagatta
     4021 catagatgta gttataccag gtatggagag tgttgaacag gttagacaaa atgtcgctgt
     4081 acttgaaaat cttgttctag atgaaaaaga taataaggaa attgaagaaa taagaaattc
     4141 tttaggtaaa aaattctgta gaagatgtga atattgtatg ccttgtgctg taggaataaa
     4201 tataccattg agttttttat gtgaaggtta ttatactaga tatggattaa aagagtgggc
     4261 taaagaaaaa tatgaggtta tggatgttaa acctactgag tgtattgact gtgggctttg
     4321 cgagagtaga tgtccatatg aacttcctat aagagaaatg cttaagactg ttgttgaaaa
     4381 gctaggatag attttattta ttgaaatcgg tgtattaaat ttactccgat ttctttttat
     4441 tagatgtggt cttatataca aatattaaat ttacatagtt atagctatta tataaattta
     4501 atatttgttt aatacttatt aaatatttgc ttaatgcttt ttaaaaacat ttgtttgagt
     4561 atagacataa cacaattatt attgttgtat tagtatagtt tcaaatactg gagtatcaat
     4621 ataatgtcga ataacacata aaatctttat ttttcaaaaa tattatagga tatatcgtat
     4681 ttagatactg gataaagttc aatttcaacc ctagggttat ctttatctat aaaatcaaat
     4741 aaaaatactg gcttaagttg tttatcattt tttattattc cacttttttc aataccatca
     4801 caaatacttt ttggataatt atgtaaatcc cccattctct tatttggaaa gaataatttc
     4861 aagactgtta ttagattttc ttcaacagtt tccccttgat actgttggtt tataaaacct
     4921 gcaatcatgt tttcgtatgc aagatactta gaatattttc cctttgaagg catccatgct
     4981 tgaccattta tgttatgaag cttaaaatta gatttactaa taggtctacc tggaattgtt
     5041 attttaatca aatttttcac catccttata cagtatttat tataatctgt atatgattta
     5101 aaagcaaatt gttatttaaa gtgtttttac agggcttgtt tgtaaaattt tccactttag
     5161 atgtagtaat ataagataag tattttgctt acaaatttta aaatttgata tatgatggac
     5221 atattcatat tagactatta taagaccttc ataatattaa taagtatagg tttagtaaag
     5281 agagtaaaaa gaaatccata taagccaagt aaagacttaa taaatggcaa tgtaaatggg
     5341 atattaatta taagaaaaaa tcaatctttt tgcattaact ataggaagaa tgtcaaaata
     5401 taactaatga tatttgaagt tttagagttt atattaaaag gtaagaaggt taagtgggta
     5461 aaaaaggttg aatagattaa aaaggctaag tatctaaaag agttaagtag gtaaaaaagt
     5521 taaataagta aatgaaaaaa gctaagtatc taaatgagtt agtagataaa aaaattaaat
     5581 agataaaaag attaagtatc taaaagcgtt agtagataaa aaaattaaat agataaaaag
     5641 attaagtatc taaaagcgtt agtagataaa aaaattaaat aaataaaaaa gactaagtat
     5701 tcaaaagagt tagtaggtaa aataattaaa tagataaaaa gactaagtat ttaaaggagt
     5761 taagcagata aaaaagttaa ataagtaaat gaaaaagacg aagtatctaa aagagttagt
     5821 aaataaaaaa gttaaataag taaataaaaa agatgaagta tctaaaagag ttagtaggta
     5881 aaaaactaaa taggtaaaaa gacgaagtat ctaaaagagt tagtaggtaa aaaagttaaa
     5941 taaataaaaa agacgaagta tttaaaagag ttagtagcta gaaaaggtta aaaagactaa
     6001 aaaaattaaa taaatttaaa aagtcaatta gattaaaaag gaataatttt atggataata
     6061 aggaattaaa agcattttgt aatttaatag gattaaactg tgtaggagta gcaggggtag
     6121 ataagtatga taatttagaa aagatactta aggacagaca acaaaaaggt tatttaactg
     6181 gtatggaaga accaataatt gagaacagaa ttaacccaag acatataatg aaggatgcta
     6241 actctataat agtctgtgca tttccatatc atataaatag acaagaaaag aataaaaatt
     6301 caaatttatc aaaatattgt catggaaagg attatcacat tgtagtaaag gactttttac
     6361 aacaaatttg tgattatatt tctaaaacag taacagattt taaatacaag ttatttgttg
     6421 ataatggacc attagttgac aggtatttag cttatttatc aggaatagga tattttggaa
     6481 taaataataa tatcataact gatgaatatg gttcttacgt atttataggg tatatagtaa
     6541 ataattataa atttgaagaa gataaaccac tagaaaaaag ttgttttaaa tgtggtaagt
     6601 gtgtaaaata ttgtccggga aatgctatat tgggaaatta tgatatgaac cccaaaaaat
     6661 gtttatcata tataactcaa aaaaaaggag atttgtcaga gaaagaaaaa gttgttttaa
     6721 agaatggcaa aaaggtattt ggatgcgata tttgtcaaga agtatgtcca cataatgttg
     6781 aaatgccaac aactcatatt ttggaattta aagaaaatat aatagattat ttggattatg
     6841 atgagataca aaatatttca aataaagaat ttaaaaggag atatggagat agagctttta
     6901 gctggagagg gagaaatata ataaagagaa atatggaaat aattttagat aagtatgatg
     6961 actaataata agagtctgtt tcatatactt taatgtgtat ttactttaac acttgttgtg
     7021 taaaagtcat attatatcat taaagttaaa tatggggagg tgggggaata ttgaaatata
     7081 ataatacatc tgaaataaat ccactagatt atatattggg tcaagaaaga gctgtagaag
     7141 ctatggaatt agggttgaaa ataaataatc cagcgtacaa catatatata gctggtgaac
     7201 ctggaactgg taaaagtact tatgccttaa aggttttaaa tgaatatgca tccaaaaaaa
     7261 atacacataa agattggtgt tatatatata attttgaaaa ccctagagag cctattatag
     7321 ttgaattaga aaaggggttt ggaagagaac ttaagaaaga tatggagaaa ttaattgaaa
     7381 gtcttttaga agattttaaa aatgcgtttg aaagtgaaaa ttttgaaata gaaaaaaata
     7441 gacttctaga tgaatatgaa atagaaaaag atatgctttt gaagcaaatt aagaaatatg
     7501 gggaagaaaa aggatttaaa ttaaaacaga gtaaaatggg aatagttttt atccctttga
     7561 aagaagagga agatgaaagt gataagtctg atgaagagtt ttacaaagct aaaagagaac
     7621 tagaaaatat ggcgatacag gtcgtatata aaattagaaa cttagaagaa atagctgaaa
     7681 aagcagtgtt agagcttgaa gaagagatag ctaaacttgt agtagaacct catatcaaga
     7741 gattgtgtga aaaatatgaa aattatgata agattcaaac ttatctggaa aatataaaga
     7801 aagatataat agaatatatg tatttgttct atttagatga agaggagtta aaagataagt
     7861 atgataaaga gcattttatt aaatacaaaa ttaacctatt tgtagacaat gaaagctcaa
     7921 agaataaaga atcagcacct gttgtagtag agataaaccc aagtccagca aatctatttg
     7981 gaaaggcaga gtatgattat gcaaatggaa atataaagac tgatttcaca aaactattat
     8041 caggagcatt tcatagggct aatggtggtt atttagtact ttatgcagac caattactaa
     8101 aatatacaat gtcttgggaa attcttaaaa agactttgca gactaaaaaa gtaatattgg
     8161 aaacacaaac agcaattaaa ccagaaaata tgccattgga tgttaaatta gtactgattg
     8221 gaagccacta tatatatgat attttatata gatatgatga agattttgaa aaatatttta
     8281 aagtatttgt agactttgat agtgaaatgg ataaaaatga agataatgaa gaaggaatag
     8341 caagatttat agcataccaa tgtgataaga ataatttaag gcattttact aaatcagcag
     8401 ttgaagaggt tattaaattt agcactagat tatctggtga tttagaaaaa ctatcaacta
     8461 agtttaataa aataatggaa gttgtaatag aaggaagtgc ttatgcagag tttagaaatt
     8521 cggagtatac agagcagtgt gatgttaaaa aagcaataag tgaaaagaga aaaagaatca
     8581 atagagtgga aacacatatg gatgaatcaa ttgaaaatgg atttactcta atagaaacag
     8641 agggaagaaa agtaggagtg ataaatggtc tgtcagtctt aagtacagga gaatactctt
     8701 ttggtagagc ttcaaggatt acagcgacca ctagcccagg aagtaaaggc atagtaaata
     8761 tagaaagaga agtaaatatg agtggctcta tacataacaa aggagtgctt atattaggtg
     8821 gctatctttc tgagaatttt gcacaggatt tactattgtc actaaacgca tatgtttgtt
     8881 ttgaacaaaa ttatggtgga atagatggag atagtgcatc ttctgcagaa ttatatgtac
     8941 tattatcatc tcttagtgga gtatcaatta aacaaaatat tgctgttaca ggttctataa
     9001 atcaaaaggg tgatatacaa gtagttggtg gtataagtga aaagatagaa ggattttatt
     9061 ctatatgtaa aactaaaggg ttaaatggtg agcaaggcgt cataattcct agaaaaaatc
     9121 aaagaaatct cgttttatca gatgaagtta atgatgcagt gcgagatgga aaatttaaga
     9181 tatatactgt tgaaagagta gaagaggcta tagagatatt gacagatgtt aaatttgaag
     9241 aaataaagaa attggtaaaa gaaaaattaa tatctttcag caagatacaa actgtgtcaa
     9301 aagaataagt ttattataga atttaatggg tatttatgta taataaaaag caagaacttt
     9361 aataggtttt tgcttttttt gtaaatatat ataaattaaa ttcttatttt gtatgtttta
     9421 tatattgatg tttatttttt gttgatatga ctaaactatt tataaatagt gttttataaa
     9481 ttggtatttt aaagggggat aattatgaaa aatgtaaatc taaaagagat aaatgaaaaa
     9541 gatgtaattg tggaagaaga tataaatcta aaaggaatgg ataagaaaga tgtaattgag
     9601 aaggaagaag taaataaaaa aagtatagat aagaaagata taattgagaa agaagagata
     9661 gataaaaaaa atgtaaagaa ggttgaggtt caaaaagaaa tagataaaat gattagtaaa
     9721 aaagatgaga taattgatga gaaagatata gagaatttta ggtatattga agaagataaa
     9781 aaaagtatca aagtaagtaa aaaaagtact aaaaaaggtg aaacggagaa aaaaacaaag
     9841 aaaagtagtt ctaataaggc tacacaaaaa caaagcaaag aagttaaaag taagtctaaa
     9901 attaaaagtg aatcagccaa taaaaaagat gtaaataaga ttttagatga gttggaaaaa
     9961 ttgtacccag atgctaaatg tgagttaaat tatgggacag cctttgaact tttaatagca
    10021 actattttat cagcacaatg tactgatgta agagtgaata aagttacttc agagttgttt
    10081 aaaaagtaca acacagctag agactttgct aatttgagca ttgaagaaat tagtaaagag
    10141 ataaagagtt gtggtcttta caaaagcaag tctcaaaaaa taaaggatac atctgaacag
    10201 ctatgtgaat tatatgatgg tgaagtacct gatagcttag aaaagcttat aaaattacct
    10261 ggtgttggaa gaaaaacggc aggggttgta cttagtaacg cttttaatca tccagcaata
    10321 gctgtagata ctcatgtatt tagagttagc aatagaatag gaatagtgga cgagccaaat
    10381 cctcaaaaaa cagaatttgc acttatggaa gctataccaa aagaaagatg gtcacactca
    10441 catcatgtat taatttttca tggaagaaga atgtgtaaag ctagaaatcc agaatgtgca
    10501 agttgcccaa taaaagaaga ttgtaattat tataaagaac ttaatgaaac gaaataactt
    10561 ttagaaagta agttgtacaa gagggatttt ctgatataat aatacttgtg aaaatgttag
    10621 ataaaaaact attatataca tgaaataaaa attattatta acattatttt atataatagt
    10681 ataatattat tgttattctg tttataaaat aaaatttgat aaataaaagc gatacttagg
    10741 gaggattaaa atgtcattaa atatagtatt agtagaacca gaaatacctc aaaatacagg
    10801 aaatataata agaacctgtg cagcaacagg ttcaacttta catttagtaa aaccactggg
    10861 tttttcattg gatgataaac acctaaaaag atgtgggctt gactattggg atatagctga
    10921 tatacaatat tacgatagct ttgaagaatt acaagaaaag tatccaaata gtaaatactt
    10981 tttttctaca acaaaggcta aacagtcaca ttcagatgtg aaatatgaag aaaattgttt
    11041 tatagtattt gggaaagaaa caaagggatt accagaacct ttgttgaaag caaatatgga
    11101 tacttgtatt agagtgccta tgttggatgt tgaaaaggct aggtcattaa atttatcaaa
    11161 ttcagttgct attgtagtgt acgaggcttt aagacaaata ggatacccaa atatgaggta
    11221 gtttcaacaa gcctaaatga gaaaggagca tacttaatga aaaatctaaa gataatctgt
    11281 gacagtttat cggacgtgcc aaaagatttg ttagataaat atgatataga tatagtttca
    11341 ttaacagtta tactggagga aagagaatac aaggatagaa ttgatatatc tggagatgaa
    11401 ttttataaaa ggcttagaga agaaaatgta tatccaaaaa cttctcaagc tacttatgcc
    11461 caatttaagg aaatttttga gaaatataca aatgaggata gagagatact ttatatagca
    11521 gggtctgcca cagctacagg gacgtatcaa agtgctgtaa tggcaaagaa tgatgtggat
    11581 ggtaaaatat atacatatga ctctaacttg ctttgtatgg gaactggttt acttgtaata
    11641 aaagcaggag aacttgcaag tgaaggaaag tctgttgatg aaatattacc tgttttggat
    11701 gaattaaaag agaaggcttt tgttatattt tcagtagata cattggaata tttacaaaag
    11761 ggaggtagaa tatcatctac taaggcagca ataggaagta tattaaatat aaaaccgatt
    11821 cttgaaataa gagatggact agtttcgcaa atatctcaag taagaggaaa gaagaatgta
    11881 atttctaaga tgatggaatt gataaaatca aattgtgggg atgatttatc agaccaaatt
    11941 gtgtatatag gatatagtga tgattttaag gaaaaagaac aattatctaa ggttatgcaa
    12001 gatgaattaa gacctaaaga aattagattt ttccaaattg gtgtgtgtat aggtgcgcat
    12061 gctggtcctg gtgtcactgg tatcatatgc ttaaaaaata aataatatat ttttggatga
    12121 cattatttat ttttttatta aattaggata gaatattaat tattgagatg ttaaattgga
    12181 tttattttgg atgaaattaa gtgttgatat attagattta gtttgtaggg aagtgtgtaa
    12241 atagataaga atctattgca cactttttta tagtaagatt tattaaaaaa atttcaaaat
    12301 gtacttgtaa attgtatata aaaaaattat aaataggatg taaaagtaaa taggatgtaa
    12361 aagtaaatag gatgtaaaag taaataggat gtaaaagtaa ataggatgta aaaatatcgt
    12421 aaatgtatga ggagtgaaat ttaatgcgtt tcaaaagaat attgatattg atgttaactg
    12481 tagcaatgat agctggaatg ttggttgggt gtgctaataa cccaagcaat aatcctagct
    12541 ctagcaaaga taataaaagt gatataagcg ataaaagtaa ttcaaaaaaa tctgtatcaa
    12601 tgatactaga tattgaagga acaaataatg aagctatgaa taatgcagct ttattagcat
    12661 taaacaatgc tcaaaagaaa cttaatatag atacaaacaa agtggaatca gatgattcct
    12721 caactttttc taatagtata gatattttat gtaatgacaa ttacgattta attatagctg
    12781 taggagctag atttgcaaaa ccattggaga tggtagccaa gaaatatcca aagcaacaat
    12841 ttgcaattat agactatgag tatgacaagc aaccttctaa tataacttca atttcttatg
    12901 aagataataa atctggatac ctagctggat taattgctgg aaaaatgact gaaagcgata
    12961 aggttggttt tataggagga ataaagagtt ctagtaggga taaatttgaa tcaggtttta
    13021 gagagggtgt taaattttct aatagtagta taaaggatat ttcagttgag tatgcagatg
    13081 tttttaaaga tagcaaatca gtagaatcta tagctaaaaa aatgatggat aatggagtgg
    13141 atattatttt ttctactact gaagatgatt caaaagctgt aatagatgca gtaagggcaa
    13201 aaaataaaaa agttatagca acgaataaag accaacatga gttagctcca gaaaatgtta
    13261 taagttctat tgtaaaagat tttgagaatc ctacttataa tctgatacaa agttttgtaa
    13321 aaggaaatta taaaggcgga aaagttatag aaaacacagt taagtctgga agtactgggc
    13381 ttgcccaaaa tagttctcaa aatattcctc cagatgtgtt agaatatgtt aataagaaca
    13441 ataaataatc tactataata tccaagaata cttatgactt tagttatgag gtgaattgga
    13501 tgttataata gacatatata gaaagacgta tgcagaggta ggcagaccta tctaaatcag
    13561 ggagtgaatt ttgtgaaaat aaaaaggata ttgatggctt gctttgtagt agtttttaca
    13621 atttttatat tagctatatg taactttgat tttggatttg ataaagtaaa taagaataat
    13681 tctaatatag atagtgctaa atcgaaacag agtgagtcta aaagtgtacc aacttcagca
    13741 gaaaaggata aattaaatag taaaagtaag aatgaagaag atgacacaga agatgattca
    13801 tatgtaagaa aaactattgg agtattgtta gataccgaaa acttaaataa cgaatcatat
    13861 aataataatg tactatcagc cttaaaaagg gctgatgaag attttgatgt taggataaaa
    13921 cttgaaaaac caaagacttc taaaagtttt aaagagagta tagaaaaact acataagtat
    13981 gaaccagatt tgataatagc agttggagct agatttgcag agccaattaa agaagctgct
    14041 actaaatatc ccaaacagca atttgcaata gttgactata cttataaagt tcagccagct
    14101 aatgtaattt ctgtatcatt tcaagataat gttactggat atttagcagg gcttattgca
    14161 ggtaaaatga ctgatactgg gaaagtcggt tttataggcg gagttgaggg ttctagtaga
    14221 gataagtatg aatctggatt tagaaagggg cttaaaactt caaataaaga tgctgagtta
    14281 ctagttaagt attcaaatat atttgaaaac cctgaatcag ctagaactat tgcaaaggaa
    14341 atgagagatt ctgatagaga tgttgtattt tcagctacag aagatgatag taaaagagct
    14401 attcaggtag caagagaaaa tggtggtaaa gttatagcta ttaataaaga ccaatacgat
    14461 atggctccag ataatgtaat aagttcaatt ataaaagatg tagaacaacc tgtctatgaa
    14521 ctgataaaga acgttgcaaa agatggtttt aagggaagta aagtaatgga atttaaaata
    14581 aaagatggag aacttggctt gacttcaaag agcagtagaa atataccacc agatgttttg
    14641 gaatacataa aaaaagagta taacaaagtg aaagatacat attaattatt gatgtagaag
    14701 attataaaaa tatactgttt aagtttagat tagcaataaa aagatatatt tattaaattt
    14761 gtgctaactg tacaaaatat attgattaag tttagattgg tgataaaaag atatatttat
    14821 taaatttagt ctaactgtac aaaatatatt gattgagttt agattagcaa taaaaagata
    14881 tatttattaa atttgtgcta actgtacgga atatactgct taagtttaga ttagtgataa
    14941 aaagatatat ttattaaatt tgaaccaact gtacaaagct atactaatta agcttagatt
    15001 aactatataa aattataaca ataagtgtgt gggcagaaaa attctgctac acactttatt
    15061 tatattcata taaaaaaatt atcaaaaaaa agaaaaagga gtaaaaaatg gaatttacaa
    15121 gagaaccatt tatactagat gacgatttgt gtctagcttt ggtggacaaa agaattagtt
    15181 atctaatgga aaatgaatta aaattaagaa atatagagat aataaaaaca atagaatgta
    15241 aagaattata tgaaagcata aaatatcacc cggatatatg tatttgtaat ttaggaaaag
    15301 gagatataat tgttgcgccg aatgtatata atcaatacaa agagttatta agtagatata
    15361 aatttaatat aattaaggga gaagctaatt tattaaatag atacccttat gatatacctt
    15421 acaatattgc gatagttggt gactatgcta tacacaattt taagtatact gacaaagcaa
    15481 tattagatta tattgaaaat aataaactta aaaagataaa tatagagcag ggctattcta
    15541 aatgctcaat ctgtatagta gatagaacgt ctataataac atcagataaa ggtatttgga
    15601 aaagtatgaa gaatacagaa atagagtgtt tattgataga aaaaggtcac ataaatttat
    15661 ttgagatgaa ttatggattt ataggtggat gtacagggtt gatttctaag gataaactag
    15721 ctttttgtgg agatgttaaa aaacatcctg actatgaacg tataaaaagt tttgtagaaa
    15781 gtaaaaacaa ggagatagtt acacttagct gtgaaaattt actagatata ggttctatag
    15841 ttcctctcat gacaagaaag gagcgataac tttggttgag aaggataata tttcttccat
    15901 gaaatttttg aggaataata atgtaaacga tagattaata tatacgaatg acattgtaga
    15961 agtagtgata gattctgcaa agcaagaagt gttaaataaa aaaatactta atggtactaa
    16021 agctgaacaa agagctttaa atattgatga gatatcaacg gaaataacta gtatgataat
    16081 aagtataatg aaagagaaac ttttgaaaga atacattcta aaatgctatg ggcgtacata
    16141 tcctgaagat aaaaataata tatacatata ttcactgaac atatttgcta aaaaagagat
    16201 ttttatgaga gaaacagtat ttactagggt gagaaattat atagaagaaa atgattttat
    16261 aaatgtagaa ggttttatta gatttagaat gagagaattt atgaaatata tttctgctat
    16321 aggtgatatt gcagttgagg agtatttaat aaagaaagac caagatgaat ttataagagt
    16381 tttaaaatac tttatagata ctcaagaaga aaagattgat ttgcttaaag ttcatataat
    16441 ggaagataat acatttgttt tatacgataa aaatggaaat aagatagaca gtatagatga
    16501 tgaagaaatt ataaatatgg taataagaga aaatttaaat tatgaggact ttttaataag
    16561 taccttatta actctatgtc ccaaaaaaat agaaatattg gatttgttaa ataataattg
    16621 ctcaaaggaa attatagata ctgtagaagc aatttttgaa aataaagtta gtataattat
    16681 ggaaaactag aatctaagca tataaaagct agccatataa aattaaaaaa attcttttta
    16741 aatcgacaca aaagacttat aattgtatat aaagctatag tataactaga gttattaagt
    16801 ataaagaata tctacttgat taatgcaata tagctggcaa atatacaatt gataaggagt
    16861 aattttataa tggataattt aattaggata ctgatattag ctgttattgg tggatttata
    16921 ggttatgtta cgaatgtagt tgctataaga ctgatattta gaccaataga gcctattaaa
    16981 atacctattt taaatataga aatagtaggt cttataccaa agagaagagc agaaatagca
    17041 gcaaatgtag gagaaataat tcaagaagaa tttctttcta tggacgaaat acttgcaaat
    17101 atcattactg acgaagataa ggaagaagtt gtaagataca taaaagcaag ggtaaaaatt
    17161 ataatacatg agaaagtttc atttatcccg agtggtataa aaaacatgat acaagattat
    17221 ctaggagaga taattgagtc tgaagttaag caaagtatag atgaattgag taaaaatata
    17281 ataaataaag caaatgaacg tatagatata caaaaaatgg tcgaagataa gattaatgaa
    17341 ctagatttat atgaattgga agagataata ataagaatag ctaagaaaga attaaaacat
    17401 atagagtttt tggggcttgt cttagggttt ttaataggta tagctcaagg gttaattact
    17461 atgtttattt aaatatatga gaagtagcat ttatttaagt actaattata tttacctatt
    17521 tgagtgaatt ac
//
LOCUS       DAMJVX010000072        17304 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMJVX010000072 DAMJVX010000000
VERSION     DAMJVX010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.075"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            39..785
                     /locus_tag="PC407_002101"
     CDS             39..785
                     /locus_tag="PC407_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDF2497546.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            847..1521
                     /locus_tag="PC407_002102"
     CDS             847..1521
                     /locus_tag="PC407_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2497547.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCLNCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCALRCMMSCPWSL
                     RYANQK"
     gene            1889..3235
                     /locus_tag="PC407_002103"
     CDS             1889..3235
                     /locus_tag="PC407_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2497548.1"
                     /translation="MEIETKTPDWINVLELNSKIDITWKYDVSNMIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIVGTTNFITRGVSNI
                     SIITLEGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLFHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     REIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITKCGQYYKSVDYNTYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLDEHKIYVIKANETNKVMLTC
                     YAEADELIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSIEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            3262..4662
                     /locus_tag="PC407_002104"
     CDS             3262..4662
                     /locus_tag="PC407_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2497549.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSSKLQEIIDAAPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GEPGHYENLFFSGFSGIALDMPNNSTANDITVFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKVIKMHKPEVGDIMSTIIKDENILSAHYGGTWGKIGE
                     KIEFGITVHYYKLIEK"
     gene            4872..6308
                     /locus_tag="PC407_002105"
     CDS             4872..6308
                     /locus_tag="PC407_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2497550.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPDLQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSPTETTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNEEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKDYEGYGSYKINNITIDQCGYCGFLFDSVKGSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGKETVYYYKHHEPDKTNQIK"
     gene            6652..7596
                     /locus_tag="PC407_002106"
     CDS             6652..7596
                     /locus_tag="PC407_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HDF2497551.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            7765..8697
                     /locus_tag="PC407_002107"
     CDS             7765..8697
                     /locus_tag="PC407_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDF2497552.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8802..9269
                     /locus_tag="PC407_002108"
     CDS             8802..9269
                     /locus_tag="PC407_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HDF2497553.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGETMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9513..10367)
                     /locus_tag="PC407_002109"
     CDS             complement(9513..10367)
                     /locus_tag="PC407_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDF2497554.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            10533..12497
                     /locus_tag="PC407_002110"
     CDS             10533..12497
                     /locus_tag="PC407_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2497555.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLSEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVAADQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSCCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFVKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLLEESI"
     gene            12497..12958
                     /locus_tag="PC407_002111"
     CDS             12497..12958
                     /locus_tag="PC407_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2497556.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSDAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            12961..13281
                     /locus_tag="PC407_002112"
     CDS             12961..13281
                     /locus_tag="PC407_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2497557.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13297..14397
                     /locus_tag="PC407_002113"
     CDS             13297..14397
                     /locus_tag="PC407_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2497558.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14517..17117
                     /locus_tag="PC407_002114"
     CDS             14517..17117
                     /locus_tag="PC407_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDF2497559.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKHVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDYILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNVFIEESGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLILKDSGNAGDTYDFSPLKNDEDIKLSFDRVYTEKTLGYEKLVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPEKY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         6881 a         1786 c         2993 g         5644 t
ORIGIN      
        1 tttatgaaaa atgtaaattg tgaaatggag gtagtaacat gaaaattgaa aatctttcta
       61 ttgatgatat taaacgaggc tatatattta atgatgattt aaattgttat agttgcatta
      121 aatgtacaaa aacatatgag gtaggagaaa tatataaaat aggtaataga ttctttgaag
      181 cttcaaaagc tattgaactt catatggaat tagaacatag tgattattta gagcaattgc
      241 ttaatagtga ttcaaagtac aacacattga ctgaaaatca aaaacaatta tttcaattat
      301 ttattagtga tttaccagat aaggagatag caaaacaggt tggagtatcc acatctacta
      361 tacgccatca aaaatttatt ttcagagaaa aagcaaaaca ggctaagctc tatttagcac
      421 tatatgaatc tgtatttgaa gataaatcta ataaaagtag tactataata ccaatccatg
      481 aacatgctcg aatggtagat gaaagatatg tagtaactga agaagagaga aaacacattt
      541 tggatacatc atttgaaagt atgaatcctt taaaattaaa agtattttct tcaaaagaaa
      601 agaaaaaggt cgtaatttta tctaagattt tggaacaatt tgaacgtggg aagaaatatt
      661 cagaaaagga agttaatcag attttagaat ctatatatga tgattttgca acaattagaa
      721 gatatttaat tgagtatggc tttatgagta gaaataaaga atgtacaaag tattggttaa
      781 cataattaaa ataataaatt ttactaatgt gtaatgttat ttttatctag gggggaggag
      841 ttaaatatga atgatgaatt aaaatctatt ttatacaata aaggtgtaga cattgttcgc
      901 tttgttgata tatctgaatt tccaataaat caaacacaag gtttttctaa agcaatttta
      961 ttttgtatag ggttatcaaa aaaatttatt acagatatat acaataactt acctacagat
     1021 agtgatgagt ttttggaaaa agaggagaaa gtagaagaat tagctgactg gatttcaaaa
     1081 tatattcaaa ataaaggata tagagcgtat tctcaatcgg agaaaaacaa tctagaacat
     1141 ggatattttg aaaaagccta tattaatcca gaaatgcaat cagggattag tcctttacca
     1201 cataagacaa tagcaaatat tagtggcatt ggatttatgg gaaaaaataa tttgtttgta
     1261 acagaggaat acggatgtgc ttttagcatg tgtacagtac ttacagatgc tccaatttct
     1321 gttgaaagat atcctttgat tgattcaaaa tgtttaaatt gtaatgtttg tgtagagaac
     1381 tgtcctgcaa aagctataca tggaaatgaa tggactttac ctggaaaaag agaatctatt
     1441 attgatgttt caaagtgttt ttgtgcattg aggtgtatga tgagttgccc atggtcatta
     1501 agatatgcaa atcaaaaata actttactag agaaggtatt aaattgaata ttataaaatt
     1561 tttaaaatgt aagattatat aagatttctc aaaataaaaa tttgtataga acactcagaa
     1621 atgagtgttt ttctattggg attaaattat attgatacaa agcttaacta ttttaatcat
     1681 tatataaaat ttgtattaaa aaattaagaa taacttgcta atagtaaaaa tataatgaga
     1741 ttataattga tttataatat aactgtaatg atgttgtaaa atgaaaattg tcatctcaaa
     1801 attcccaaaa agagtaaaag tcacttcggt tatagtcaaa aaattttttc tgtgaaaaga
     1861 atttagattt aaaagggagg aattaatcat ggaaattgaa actaaaactc cagattggat
     1921 taatgtacta gaattaaaca gtaaaataga tattacttgg aaatatgatg taagtaatat
     1981 gatacaagat attgtctctg ataagtcact agaaggttcc attatatatt ttccaaaagg
     2041 taattactta tttgagaaag gtattaaaat tagtcaacaa ataacacttc aaggagattc
     2101 ttattatgga ggagataatc aagtatcaaa tttagataaa aaacaatcaa tcgtaggaac
     2161 aaccaatttt ataacccgag gagtttctaa tataagtatt attacattgg aaggaacaag
     2221 tcaatgtatc aaaaatatta atttttatta tgatagtcat gatatagaga aaataccacc
     2281 taaaaatgta tctgctatta cagaatatgg agaaacacaa ggactatttc actttgaaca
     2341 cctgtttatt tctggatttt caggaatagg gattgaaatt ccatactatt caactggcaa
     2401 tgatatcatt gttagttcat gtggtcttgg tatgaggctt ggagagaagt caatgctttc
     2461 tagtagcaag atttatgaat gtaaaaatgg aatggagatt actactggtg ttagcttaaa
     2521 taatgtgaga atagaagaaa taagagaaat aggtattaat aataaaggat ttggttttaa
     2581 cctgattatg aatcttatgg tcgaccaatg tggatattgt ggatttatgt ttgaaaaaat
     2641 gtgttatagc cagataactg ccagaattac taaatgcggt cagtattata aaagtgttga
     2701 ttataatact tatgagaata tggaagatag aaaagaagaa gcttattcca ttttttatgg
     2761 agatatttta gaaaattgca atattgtatt gatgaatagt aatattgata ccttagaaaa
     2821 tgattcactt gatgaacata agatatatgt aatcaaggca aatgaaacaa acaaagtaat
     2881 gttaacatgt tatgctgaag cagatgagct tatacagtgc gcaaagggca acttgttgct
     2941 agaaaatggt gaaaatactt ataaatttta taatggtgag atttgttctg taggaggtgt
     3001 tcaaatttct gatatagatg aaaaagattt aatcaaaata atcgagaaca ctctgtatat
     3061 agatgataaa ggtaagtact taatggttcc tagattaaaa aagaatactg taatatattc
     3121 agtactagat tctatagaag aaataaataa attacatggt ggaatatggg aacagattga
     3181 tgtaaaagta ttagctgggg aatctatgta ctattataaa aagataaaag attaaattat
     3241 caaactagga ggtagtgatt aatggaagag tctataataa ttaaagatat aagtggctgg
     3301 attaatgtac ttgattatgg gatttgtaat gatgaaacaa aagatgtaag ttccaagttg
     3361 caagagatta tagatgctgc accagaagat tctattatat attttccaaa aggaaaatac
     3421 ttatttgaaa gtggtgttaa gattaagaaa cgattaacct tatgtggaga ttcttatatt
     3481 ttggcgaatg ttcctaaaaa cactgctgga gtaacacaat ttacttttaa aggaaagaaa
     3541 aatcctaaaa gtgaaaatat agcaattata acagcaaaaa gtgtaagaca ctgtattaag
     3601 agcatttgct tttattcaga tagttgtgat agagatatga catctaatca tgaacctcca
     3661 acagatggaa aacctagatt tcatcataat gtaattataa attatagaaa tatatcggct
     3721 attgtatgtg aagataaaca gggagaacca ggtcattatg aaaacttgtt ttttagtggt
     3781 ttttctggaa ttgcacttga tatgcctaat aattctacag caaatgatat tacagttttt
     3841 acatccggac ttggaattag tacaggggaa aattcaacga ttgctaacag taaggtttgg
     3901 ggctgtgttg atggaatgat aatttctact ggaactttta taaatggtat gagggtagaa
     3961 gaaataaaaa aagttggaat taaaaatatt ggtagaggtt taaattttat tacaaatatc
     4021 acaattgacc aatgtggata ttgtggagtt tggtttgatt ctatatccaa tgttcagata
     4081 tcaggaaata tcacaaggtg cggacagtac tattatgatg taagctatga taattattta
     4141 atgttggata atagagttaa agaagcatat tctttattct atggtaattt attggagtca
     4201 agtaatatag ttttgtcaaa taccaataaa gataattggg aagatttcaa agaggataag
     4261 cataaggtgt atcttgtaga agcacttgaa accaagaatg tactattaac atgcaatata
     4321 gatgctactg actggattgt taagtcagaa aaaggaaatt taacgtacaa taatatgcga
     4381 caaactgtta tattttacga tggaaaacta agtagtatca atggaattgg tatatctgat
     4441 gaagattatg gggataaagt caagataaaa aaaggaaata tatatataaa tagtaaggat
     4501 aaggttatta agatgcataa accagaggta ggtgacatta tgtcaacaat tattaaagat
     4561 gaaaatatac tttccgctca ttatggggga acatggggga aaattggtga aaaaatagag
     4621 tttggaataa ctgtacacta ttataaattg atagagaaat aagtaaatac gtagtatttg
     4681 aatagatgta ttttattgga gagctaggac tatagtttta atgtaaatgg tttggtaata
     4741 gcatggattg aataatataa aaataatata aaaaattaat ttgattatgt aaaattctat
     4801 gaagtagtaa acctgtgact ttagtcatgg taggttcaaa ttaagtaaat gggaaaggaa
     4861 tatataatat tatggaagga agaactggga ttaaagatgg aagatggatt aatgtagtag
     4921 aaagaggtat taaaaatgat ggttcgtaca acataggtcc tgatttacag gatattattg
     4981 atgaaataga aacagatttc tgtgtcttat attttccaaa aggaaaatac ttatttacaa
     5041 gtggtgttaa aattaataaa gaaattgtat tacaaggtga ttctaattca cctacagaga
     5101 ctacacaatt tataactcgt ggagtagaaa atatgagtat tattacattg acagggaaga
     5161 aacaatgtat taaaaatata aatttctatt ctgatagttg tgagatacag gtaaatgaag
     5221 aaccaccgac aaaaggtaat cctaaatatc attatgagat gattattaat aagaatgaaa
     5281 aaaatgttga gatgaataat gtatcagcaa ttctgtatga taatagtaag aatatgatta
     5341 aaggtctagg tcattatgaa aatatttata taagtggttt ttcgggtaca ggaattagga
     5401 taccatatta ctcaattgta aatgatatta cagtttcatc ttgtgggttg ggtattgata
     5461 caggaataga cacaattatt tctaatagta gaatatctaa gtgtcaaaat ggaatgagga
     5521 ttagaactgg aactagcata aataatgtaa gaattgaaaa aatacagaaa gttggtctta
     5581 taagtagtca agttaaggat tatgagggat atggaagtta taaaattaat aatattacga
     5641 ttgaccaatg tgggtattgt ggttttttat ttgactcagt gaaaggtagt tatttttctg
     5701 gtattataag aagatgtggg cagtattatt ataatacaga ttatgacaca tatttaagta
     5761 ttaaagatag agtagaagaa gcatattctt tattttatgg aaattatttt acagaatgta
     5821 atattgattt agttagtgat aataaggata actgggatga tggcttagaa tataaacata
     5881 aagtttatgt atttaaaacc cttcaaatac caaaattaac attgagatgc aatatagatg
     5941 cctatgatta tatcgttaaa tcggtacatg gaggaggtaa tttaatgtta aaaaataact
     6001 ttaacaatta tatatttatt gattcatcta gtccagaagt tattaatgga attaatttta
     6061 attatcaaaa gaatggaaac ctgttaaggt taaataatgg taaattaagt tataaaaatg
     6121 gaaaggaaga aacagctata ttcagaccta attatggtct tattgtagca tcaacagatt
     6181 cagaatgtga acaaatagag actagctatg gaggaaaatg ggagaagctt ggttctgaag
     6241 taattggaaa agaaacggta tattattata aacatcatga accagataaa acaaaccaaa
     6301 taaaataaaa tttgtattat ataatgatat aaagttttaa tatatagtgt ttacctttaa
     6361 tgtaatattg gtatatttgt atagtattaa atttgttaaa aagtaaaatt gtagggtgct
     6421 tgaaaatagc acctttttta gtttcattat aaaattagtt ttgaaagttt aggaataatg
     6481 tatatttatt tttaaagaat tataacctta ttaaggatat tttaaatgct agaaatgaat
     6541 cagtaaatca tactggaatt atcttaaatg ttttgattaa attaagtatt tagagctata
     6601 attatattaa aaatatatta ggacataatt atattactat ggaggttaaa tatgatagta
     6661 agtgtgagta gaagaactga tatacctgct ttctatagta agtggttttt taacagactt
     6721 aaagaaggtt ttgtttatgt aattaatcca atgaatccaa aacaggttag taagatagag
     6781 ttaaatccac atactgttga ttgttttgta ttttggacaa aggatgtaac tccaatgtta
     6841 tatgatttaa ataaattaaa agactataaa tattattttc actatactat aacatcatat
     6901 ggaaaagaag tagagacatg tattttagat aaaagaaagg taatagattc atttaaggaa
     6961 ttgtctagaa aaataggcaa agaaagagta attttaagat acgacccgat tttcttaagt
     7021 gaaaagtaca cagtagatta tcatatcaaa gcatttgcga gtttatgtaa tcagcttgat
     7081 ggatttacag aaaaatgtat aataagtttt atcgatttat ataagaaaac aaaatttaat
     7141 actaaagcat tacatataaa gccaatagaa attacagaaa tagaaacatt atcaaaagaa
     7201 atttctaaga ttgctcataa gcatgggata atacttgaaa tgtgttctga agaatataat
     7261 ttaagtaaat ttgggattgg aaaaagtaag tgtattgatg atagattaat atcaaaaatt
     7321 ataggatgtg aagtagaggt aaaaaaagat agtacacaaa gagatatttg tggatgtgtt
     7381 aaaagtgtcg atataggaca atacaatact tgcagacatt attgtctgta ttgttatgct
     7441 aactttaatt atgcaaaagt agaagaaaac tgtaggcttt ataaagaaga taataaatta
     7501 ctcatagata ctttaagaga tgatgcaaaa ataagcatta gagatatgaa gactattaaa
     7561 gtagataaga ataaacaaat tagtatattt gattaaaata aatatactaa ttaccttaat
     7621 atatcaagaa tatagatact tttgataaca aaattaaact taatagatat tttctatata
     7681 agaaagatgg tcataatatg tcaatttgac catctttttg atataattaa aacacaaata
     7741 atttagtaac aaaggaggca gaacttgaat ttattaaaaa aatatcctat acctattgca
     7801 agtttgattt tgtcaatttt tactttaggt aacttattac aatcttatag tgaatcatta
     7861 cgtaatgtaa ttggatttat aggatttatt ttgtatgcaa tttatatagt caaaataata
     7921 ttacttagaa agggtgttaa agagcaatta gagaaccctt taatatcaag tacatttcca
     7981 actataacaa tggcaacaat gatatttgct acatatataa aaccattatc tttagaatta
     8041 agtattatga tttggagcat aggaattgtg ggtcatcttg tgttaatagt tttatttagt
     8101 aagaagttct tagttaaatt ctcaataaaa acggtatttc catcttggta tatagtatat
     8161 gttgggatag ttgcagctag tgttacggca ccagcattta atcaactttt gattggcaaa
     8221 attgcttttt ggataggatt tgtatcttat atagttctta taccagtaat tttaaagaga
     8281 gtatggataa tcaaggaaat gcctgaacca tctttaccaa gtatagctat attagctgca
     8341 ccagggtcac tattattgtc tggatatatt aattcttttt cagataaaaa taatgtagta
     8401 ttatacttgt tattcatatt atcaattgta ttttatatta tagttttggg atacttaccg
     8461 aaattattaa gattgccttt ttatccgagt tttgctgcat ttacattccc tatggcaata
     8521 agtgcaatgg gtatgaaact aatgaatggt tatttaacta aatcaggtca ttttataagt
     8581 tggatttcat atgcttctaa gttagaagag ataattgcta cagtgattat tttatatgtt
     8641 ttagttttat ttattatata tcttagtaaa tctgaaacta catctaaagc taaataagaa
     8701 ttattaaata tgtgtattta ataggaatat tgatataaat atatgaagat aagataaacc
     8761 atagaaactt aatttagctt aattttattg gaggaaaaca aatgaaaaca aaagaagaat
     8821 atttaaaaga agttagaaac atatcagaag aatactttag aaaaggagag tttttttgct
     8881 ctgaagcagt tcttcagaca ataaatgatg ctttaggcca acctttaagt ccagagataa
     8941 ctaaattagc atcaggattt ccgataggac ttggaaaagc tcaatgcctt tgtggagcga
     9001 tatctggagg agagatggct ttaggtatag tatatggaag agtacatgga gaaactatga
     9061 atccaaagat gtttgagcat gctaaaggtt tgcatgatta tattaaaaaa gagtatggtg
     9121 caacatgttg tagagctatt acaaagaaat gggatggtga taactttatg tctcccgaaa
     9181 gaaaagagca ctgtattaaa ataacaggtc aagttacaga gtgggttgca gataagctta
     9241 tagaagatga aaaacttaat atcaaatagt gtaatattag ttggtgaatt tataaagtta
     9301 aatttactta tagtataaga tacttgatat tctcgtattg atagaaaaat ttatagcata
     9361 tgtatttaat gaaatatact tatatttaga gtgtggttta atttcataaa tacatatgct
     9421 attattttaa gtatataagc aaatttattt aaataaatag aaacagttaa tttatatgta
     9481 caaaactata tatgattagt gtaaataaat acctatttta taatactcaa cataaagaca
     9541 ccagacatga ttagtattat accaataaaa atagcaagcc attctttttt gttcttcttt
     9601 tctttaaaaa atataatccc tcctaaagtg gctactatca cacaactctg agagatagaa
     9661 aaacttattg taactcccaa tgtattagtc gttatgaaca tacctaaatt tgctattgac
     9721 cacaatatac cagtaattaa attgaataaa acattttccc tatatacaat ctctttagaa
     9781 aaatacttga caataataga tgaagttatc aacataccaa cagattgagg taatataatt
     9841 ctatatcctt caattaaaaa tatttgatta gttacaacat ataatgataa aaatagagat
     9901 gataataata caactccaac acattttaac atatttttat ctgtagattt tttagtttga
     9961 taattagtga gaagtatacc aagtattata cataatattg ataaagaacc aatcagtaac
    10021 attttagaac tggtccattc attaaacaag attacagcta ataatgtcgt aaaagttaat
    10081 tgtgaaccat ttgaaattgg cattacttta gatacactag caatttgaat tgctttaaac
    10141 tgcattaatt gtcccaaact ccagaatatg ccagaaatca tacttataca aaatgaaaaa
    10201 aatgtaattt caggatgtat aaccagagtt attatagtgg aaaatataaa cgcacttatg
    10261 gatgtaccta aaagctgttc atgaggagaa cttttcttta gatttgcaac tatgggcatc
    10321 aatccccatc ccattacagg aagtaaagca ataattattg tcagcattaa actatttcct
    10381 ttcatattaa atttagacag cttttaattt atcatctaaa attaaagtgt tcaatacata
    10441 aaaattaccg tatttaatca ggaaatatta gtagtttttt agaaaaacat tttccattaa
    10501 aataaagata aagggaagga gtaaaattga aaatgttaac tgatatgatt caaaatcgtg
    10561 aaattaaaat aataaagttt ttattaaaag aaggtcatac aactgtaaaa gcaatagcag
    10621 atgatttaga tgtttcagaa aaaacagttt caaaatctct taaagaagta gaagcagtgc
    10681 ttaaaaagat aggtctttct ttaataagaa aacctaagat aggtgtttat attgatggag
    10741 atttaaaaaa aatacttcct tacctagata ataaaggaag tcaaattcct acaaccagag
    10801 aggagagagt tatctatata tattcagtac ttttaaagag taatgattat attactatac
    10861 aacaactttc tgatgaacta tatataagtc gtggaactat tgaaagggat ttatcagaag
    10921 ttgaaaaact gctaaaaaac gaagggattt ccatatataa gaaacctagt aagggaataa
    10981 agttaaatat aagtgaaaga gataaaagac aacttactgc taagtttata cataaatttt
    11041 ggagtagaaa ctggtatatt aagcaagagc aagaaagctt ttatcaagca tttgaaaaga
    11101 tacaggcaga tgtaaatggt attttttcgg aagatgattt atcaataatt attgatattc
    11161 ttaggaattt cagcagagaa agaaaatttg agttctctga ttatgaattt cagtcattag
    11221 ttattcattt ggcaatagct gtagagagaa ttagaaaagg cgaatatata gaagaaagtt
    11281 tgtatattgg taatatagaa aataatcaaa tgtcaaatac caattattta gcaaaacaat
    11341 tagaagaaaa attttcaata ttattaccaa aatctgaaat tggatatata aatttacatt
    11401 tagtagcggc tgaccaacat agagatacta atgaactaaa aaatacttca actaatcctt
    11461 gtgaaacaag tacaaataat atatcaaatc ttcgggaaat tataaaaaat acattgtatg
    11521 agacagctta tgacaaggat ttaattgatg gattgacttc acatgtacat tcatcaataa
    11581 atagactgaa atatggactt agtattaaaa acccatatgt aaatacaatc aaacaaaact
    11641 tttctttatc atttgagcaa gctttagatt taaagaaagt aattgaattt atatatgata
    11701 ttagaataaa tgatgatgaa tgtgcttata tagcattaca ttttgaagct tttagagaaa
    11761 gaatgaaaga acttccacaa aatattagag taattctagt atgcagtaca ggattaggtt
    11821 catctaggtt attgtctgca agaataaaaa aatactttcc aatgataaag attgaaaaag
    11881 tactttccgt acaagaatta gtatccagta aaattgatgc agatttagta ataagtacta
    11941 tttatttaga gttagaaaca attccaacca tagtagttag ccctatttta aatgaaagtg
    12001 atataagaac cttggaaaga aatatacaaa atgtttcatg ttgtacttct aagaagtata
    12061 aatctttcag aaacctaatc tttgaagaaa atatattgtt aaatattgaa gcttcaaact
    12121 atgaagatgc aattaagtat ataacagata acctaataaa aaaaggtttt gtaaaagaag
    12181 gtatagctaa aagtgcaatt gagagagaaa atttatctta cacgtctttt cagtcaatag
    12241 ctacacctca tgctaatcct agttatatta aaaaatctaa tatatctgtt gcaacgttaa
    12301 aaaatcctat tacatgggga aatgaaaagg tggaagttat tttctttatt tcattagaaa
    12361 atgacaaaaa cctagagctt gatgaaatat atgaagtttt ttatgacttt attgacaata
    12421 aaaaaaatat aaatgacata ttaagtgcaa cttccacaaa ggaagtgtac gatttgctat
    12481 tggaggagag tatatagtga aaatatcaga tgtattgaaa aaagagcaaa ttgtttttaa
    12541 tcttgataca aatacaaagg aagaaacaat aaaaaaatta agtagtatat ttgttgaaaa
    12601 tgggattgtg gacaatgaag aagaatatgt taagtctgtt ttggaaagag aagaacattc
    12661 aactacaggt attggtaatg gtattgctat tcctcatgga aaaagtgatg ctgtcaaaaa
    12721 atcttctata atatttgcaa agactaagca taagattgag tgggatgctt tagatgaaaa
    12781 accagtagat agtattttct tattggctat aagcaatatt gataaggata gcaatcatct
    12841 tgtactatta tctaaattag caactaaact tatggatgat gacaatgtag atgcactaaa
    12901 attagccaat tcagaacaag aaataatcaa tatatttagt gaagaaggag agatgtagaa
    12961 atgaaaagaa aaattatagc agttacagca tgtgcaacag gagttgcaca tacatatatg
    13021 gcagctcaag cgttaaagaa agcttcaaaa aagatgggtc accttataaa agttgaaaca
    13081 caaggagcta caggaatcga taatgagtta actacaaagg atgcacaaat tgcagaagta
    13141 gtaatatttg cagtagatac taaagtcaga aatgctgaac gttttgaggg aaaagaaata
    13201 ctagaagtac cagttaatgc acctataaag gatgcagaga aagtaataaa tgatgcatta
    13261 aagttaattg acactaaata aatggaggga aatgtaatgt tgaaaaagat agtctctgag
    13321 ttaaaaaagc atgtcttaac tggaatctca tatatgattc cattagtaat tgcaggtgct
    13381 atgataatgg caatatctcg tgtaggtggt tctttctata atataccaga tatatgggat
    13441 gctaaatacg cagaaagtgc tagtagtatt gttagattat tacatgatct agatggattt
    13501 ggaggtactg cattaggatt aatgtttcct gtaattgctg catttatagg attttctata
    13561 gctgataaat tagctattgt accaggtctt gttggaggta tgattgctaa agatataggt
    13621 gcaggatttt taggagcttt ggcagttggt cttatagcag gatatacatg tttgtttatt
    13681 aaaaatcatg ttaaattacc aaaatcagca gcttcaatag taccaatatt tatagtacca
    13741 gtatttggaa cattaatcac tgttgtatta atcaattatg tgataggtat accttttgcc
    13801 acattaaata ctggattaga aaattggtta aatggattat ctggtacaaa tcagatatta
    13861 atggcagcta ttataggggc tatgatggca gttgacttag gtggaccagt taataaagca
    13921 gctttaacaa catcacttgc attattaaca agtggaattt atgcacctaa tactgctgca
    13981 atggtaggaa tagttatacc accactagga ttgggacttg ctactatact agcaaaacaa
    14041 aaatataata aacagttacg tgaagctggg aaatcatctc ttataatggg tttaattgga
    14101 gtaagtgaag gagcaattcc ttttgcggta gaatctccat taaaagtaat accttcatgt
    14161 gtaattggaa cagcaattgc ttcagctatg gcagttggtt taggttcagt aaatgcaact
    14221 ccaattagtg gattttatgg atggtttaca gtagagaatt ggccaatgta tgtgttatca
    14281 atagcagttg gaacagtaat tgtagctgga ttagttatag tattgcgtgg aaatccagtg
    14341 attgaggatg atgaatttga agatgatgat tttgatgaag cagagtggga atcttaaaat
    14401 ttaataacta tttaagacgt gttataagct atattcaata tgaatcttat actaaataaa
    14461 gtgttaaata ttcagtttag acttaaatat aaaagataaa gaacagagga gaataaatga
    14521 taaaagcaca tattgttaat catacacact gggatagaga atggtatttt acttctggag
    14581 atgcattagt acttagtgaa caactattta cagatgtaat tgatgaatta gaacgtaatc
    14641 cagatgtaag ttttgtactt gatggacaac tttctatttt ggatgattat gtacaattac
    14701 acaaagaaaa aatagaaatc attaaaaagt taataaaaga agatagacta catattggac
    14761 catggtttac tcaaactgat gcattctttg ctagaggaga gtctatttta agaaacttaa
    14821 tgattggaat ttttgaaagt aaaaagtatg gtaagtatat gaaaattggg tatttgccag
    14881 atacatttgg atttaatgca cagatgccta tactattaaa gcatgtaggt cttgataata
    14941 tattcttttg gagaggtata catcttggta aacatgttca atcaccatat tttaagtgga
    15001 aaagcttaaa tggagaaagt tatatatatg cagtaaatat gccacatgga tatggaactg
    15061 gtatgctttt agagccttca ttaaagtatg ttaatggacg cttagaccca gctattgatt
    15121 ttattaaatc atatactgat gtagatgagg tgttaatacc ttctgggaat gaccagttaa
    15181 atataatagg tgatttcaaa aataaaatta aagcgattaa tgatatcgga aagtattcat
    15241 atataacgag ttcatatcaa gagtttttaa aatatataaa gtccatagaa tcactagaaa
    15301 gctatagagg tgaatttaga gaacctgtat tagcaagaat acataaatca attggttcta
    15361 gtagaatgga tataaaatta gcatgtgata aactagaaaa caagctgatt aaaagaatag
    15421 agccattatt agtaattgct aaaaagagta aaattgaaat aagcaatcag ttacttataa
    15481 atacatggaa aaaacttttg gaaggacaag cacacgatag tctagctgga tgtgtaactg
    15541 atactgtaac agattatatt cttcaccgta taagagaagc aaatgaaata tgtgacagta
    15601 ttgaaaatac tattgtaaaa aagatttcag aagggcttaa attatctaaa aatgatattt
    15661 tagtatttaa tacagaagct aagcgtttta atggatataa agagatacaa gttgtaagtg
    15721 atagtaaaaa tatttatttt aaagataatt tagatgctac tataattgaa gaaacttatg
    15781 taaaaccgag agaaaatgta ttagaagaaa cacctgctgg caacgtattt attgaagaat
    15841 caggatatta tatattaaaa gtaagaataa atgtacagct tcctgcactt ggttataaag
    15901 ttgttagttt tgaatcaagt gataaagaaa tggtatcact tgataaatct aatgacactt
    15961 ttatatctaa tgataattat aagataatct atgaagatgg aagcttaaat ttacaactta
    16021 aagatggaac ttacatatca aactttttaa tattaaaaga tagtggtaat gcaggcgata
    16081 cctatgactt ttctccatta aaaaatgacg aggatataaa actttcattt gatagagtgt
    16141 atacagaaaa aactttagga tatgaaaagt tggtggttaa aggtactact ttactaccac
    16201 ttacattaga agatagaaaa aacaaatatc ttaatgggaa attaaatgta aaagtaagta
    16261 ttgtcttatc caaagaaaat ccattgatgg atgtaaaact ttgtgttgat aacacgatat
    16321 ataatcatag actacgtgtt catataaaaa ctgatataaa agataataaa aacatagcat
    16381 cattgccatt tggatatata acaagagaaa atggagtttt agacaactgg gaagatattt
    16441 acagtgaaat gcctatagat ttagaacctt tggaaagtaa tattactcta acaaaccaaa
    16501 atagaagctg tactgtattt actagaggaa taaaagagta tcaacatatt gaagatgaaa
    16561 ttgcattgac cttacttgct actacagatg aactaggaaa gcctgattta ttatatagac
    16621 caggacgtgc ttctggtgat acaactaaaa aaggtcatat aagaattaaa acagataaag
    16681 ctcaaatgct taagaaatta gaattttcat ttgcaatata tatggattct aaatcatttg
    16741 atgaattgga aattgcgaat ttaacccaca attatctaag ggaatgtgta aattatcaat
    16801 tacaggatta caatttcttt ttatatcgta tagataataa gattcaaaaa tcaattgtca
    16861 ataaaactgt aagtagagaa ttagaaataa tatctttacc agaaaaatat cttatatcag
    16921 catgttatcc ttcttattac agtaaagata aatttataat aagagtggaa aatccaagta
    16981 gtaatagaat gatattggat gagcaaatat ttaaaaatag aaatgggaaa attgtaaatg
    17041 caatagaaga tgtagaagaa gaacaggtat atgaaatagc accttttgac gttatttcaa
    17101 tattattgga tttataagtg taaaagtaag tttattgttt aaagtctgac agatattaat
    17161 ctgccagact ttttaatttg tataaatttg ggtaaatatt aatgaatagt tttagtattt
    17221 aaatatgaat ttagtattta atatagaatt tattgaagta gaataattag tttaacagga
    17281 taagcagttt aaataaagaa ttat
//
LOCUS       DAMJVX010000073        17065 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMJVX010000073 DAMJVX010000000
VERSION     DAMJVX010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17065
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.076"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(98..784)
                     /locus_tag="PC407_002115"
     CDS             complement(98..784)
                     /locus_tag="PC407_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDF2497560.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(833..1366)
                     /locus_tag="PC407_002116"
     CDS             complement(833..1366)
                     /locus_tag="PC407_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2497561.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDA"
     gene            complement(1410..2042)
                     /locus_tag="PC407_002117"
     CDS             complement(1410..2042)
                     /locus_tag="PC407_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDF2497562.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYNTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(2132..3211)
                     /locus_tag="PC407_002118"
     CDS             complement(2132..3211)
                     /locus_tag="PC407_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF2497563.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(3344..4690)
                     /gene="pepV"
                     /locus_tag="PC407_002119"
     CDS             complement(3344..4690)
                     /gene="pepV"
                     /locus_tag="PC407_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDF2497564.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(4759..6039)
                     /locus_tag="PC407_002120"
     CDS             complement(4759..6039)
                     /locus_tag="PC407_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HDF2497565.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(6166..6426)
                     /gene="hfq"
                     /locus_tag="PC407_002121"
     CDS             complement(6166..6426)
                     /gene="hfq"
                     /locus_tag="PC407_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDF2497566.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(6540..7475)
                     /gene="miaA"
                     /locus_tag="PC407_002122"
     CDS             complement(6540..7475)
                     /gene="miaA"
                     /locus_tag="PC407_002122"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDF2497567.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDIDAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKGKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTARWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(7513..9480)
                     /gene="mutL"
                     /locus_tag="PC407_002123"
     CDS             complement(7513..9480)
                     /gene="mutL"
                     /locus_tag="PC407_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDF2497568.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKDKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(9491..12334)
                     /gene="mutS"
                     /locus_tag="PC407_002124"
     CDS             complement(9491..12334)
                     /gene="mutS"
                     /locus_tag="PC407_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDF2497569.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(12497..13231)
                     /locus_tag="PC407_002125"
     CDS             complement(12497..13231)
                     /locus_tag="PC407_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2497570.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(13200..14213)
                     /locus_tag="PC407_002126"
     CDS             complement(13200..14213)
                     /locus_tag="PC407_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2497571.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(14235..15005)
                     /locus_tag="PC407_002127"
     CDS             complement(14235..15005)
                     /locus_tag="PC407_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2497572.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(14980..15270)
                     /locus_tag="PC407_002128"
     CDS             complement(14980..15270)
                     /locus_tag="PC407_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDF2497573.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(15401..15500)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      16120..16209
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            16262..16438
                     /locus_tag="PC407_002129"
     CDS             16262..16438
                     /locus_tag="PC407_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497574.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(16553..16909)
                     /locus_tag="PC407_002130"
     CDS             complement(16553..16909)
                     /locus_tag="PC407_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2497575.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKDVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
BASE COUNT         5384 a         2928 c         1800 g         6953 t
ORIGIN      
        1 tttgatgata aaagctattt tccttctaaa taaaaaattt gtctaaagtt tctaaaaaat
       61 ttagacaaat cttaggttat tattctttaa attattttta aattattcca aatacaccaa
      121 ataatattgg tataaatata gctggtaaca aattagctac ttttatattc gttaatttaa
      181 gtacatttag accaagtcct ataattaata aacttccagc tgctgtcatt tctgtgacta
      241 caggatcact taaaaatcca tttaaaaatg atgctcccac acatataata ccttcataca
      301 aaaatacaga tattgcagaa aacattacac ctatacctaa agaagcagta aatataattg
      361 aagctattcc atctataaca gattttacaa ataaagtatc atgacttcct gtaagaccac
      421 tttctaatga accaactact gccatggctc ctatacagaa taataaacta gatgatataa
      481 aaccctctgc tatagaatct tttttatttc ctttagaaaa tttactttgt aaaaaagcac
      541 ccaacttatt tacccatttg tcaatatcta ttaattctcc tatcagagca cctacagcta
      601 cggatattat tgtcactaat gtgttttttc cttctagtgc tcctgatata ccaatgtaca
      661 atacacataa ggcaattcca ttcattatag tatcacttac tctttgaggt aatcctcctt
      721 ttattataag acctactata catccacctg caattgctag actatttgct attactccaa
      781 acattttttc tcccctcgat tagctaattg aaatttatcc tattattaaa acctaagcat
      841 ctatactttg tattggatat tttaaatatt ccaaaaattc ttcaaaacct tttctagata
      901 tatttacagt tttagtattt atgttaggat gaaaatttaa gtaatcttct ttaattacat
      961 ctttatctat gtaaagctca acttttcttt ctgtatcatt tattaatgaa aaaggattta
     1021 cacttccagg aagaagttta agtacattgt atagtctttc aggtgaagca aacgataatc
     1081 tagaacttcc tatcttatct ttaagtgagt ttaaatcaac ttgtttttct cctttaacta
     1141 caataagata atacttattt cctttggcat ttcttaaaaa taaattttta cattgacacc
     1201 cctttgttat tccatctaaa atgactaatt gctctgctgt atatacagct tcatgctcta
     1261 ctacatcata ttcaacattt aatttatcca aaatatcata tattttagtt tcattcatgc
     1321 tagattcatt ttcatcttgt tcttttttat tatttaaatc actcatactt ttctccttaa
     1381 acacttcatc ataatatcat tgtgaggtat tattctacta tagctattgc tcttactgga
     1441 gctccatcag catcattata cttaaatggt gctgctataa atagaaattt ttctggaaca
     1501 ttttctaaat ttgtaagatt ttctatgatt atttttcctt tttcaaataa aatatgatgc
     1561 acttcaaata ctgatgtgtt atatctatcc actgatagca tatctattcc tatgcctttt
     1621 atattagtat tagctatata gtttgcagct tcttttgtta atgtaggata acctacatag
     1681 tactgctttt tatcccaaaa ttttgaccaa cctgatttaa atattataaa atcactattt
     1741 tttatttcat cttcatattt ttttagatat tctaattcta tattctcatt tcccttttca
     1801 agttccaata cataagcttt cccaacaaaa ttttctatat ccaacttatc tagggtctca
     1861 cctttagtat acatatgtct tggtgaatcc atatgagttc cattgtgaga aaatacacta
     1921 attaatgttt cttgataacc attttcttca ataatagcta ctttcttgat atctggtcta
     1981 ttaggctctg catatacagg catgtcatta tgtgtcacat gagttaaatc aaaaaccttc
     2041 atctttatat accccctatg tttttatttt ctattatata cctaaattca ttttatttct
     2101 atcagatttt attccattat ataccatact actatatatt ttttataaac ctcaagggat
     2161 ttaccgaaaa atcttggaaa gaattatcag taaatataac atcttcaccc aaaaaacgtt
     2221 tataccttcc ttcttctttc tttaaagtaa ttctctgttt ttgtgaatga ttattaaaaa
     2281 ccattatctt aagaggaggt ttttttctat tagtctgcac tctttttaac atgtatttta
     2341 tgtgcatatc agcttcagga aatgaatatc cacaaaatat taaaaggtca gtatctctta
     2401 aagttttttc tgtttcattc caaacattac ttaaaaatat gtttgacata ttcttaaaat
     2461 atgttggtgg cactataata ggttctgtta actcaccaca ctctaaacat tttgtctctg
     2521 aactattttc aataagcttc atgacaccac cttcatgtgg tgtaagagtt acactattac
     2581 aaatggggca ataaagccaa ttcaatgacc catgtatctt atataatttg attaatggtt
     2641 cttttggttt tctccaattt aactcctctt taaaatttgt aaactccaca ccataatcaa
     2701 gcataattgg caaatcattc ttatctctta atccagtaac tgtattgtct atatgtatat
     2761 cataatttgc actaataaaa gtagtatcaa gaagcaaatc attttccaaa agattttcaa
     2821 ctaaaagttt atgatattta ttatctgttc taggtgtatt atgaattgct tcagccatta
     2881 gcagtattaa atattgtctt aaaaaacgta tactatcact tctgttattt agattttcta
     2941 gtccaaaatt tctaaatgct tcttttcttt gttctgctaa gtctaaaaga cctaaaactt
     3001 cttcaaaggt aggaaaattt gcattatcta tattatcttt tactatatca atattgaaca
     3061 tctgtttaaa gaaaatatac aattctctat tcattttggt attataattt gtgggattta
     3121 gttttttaaa atattcgcta aaaagttcat tttgaatagg tagattctct gctgctgaag
     3181 ctcctgctcc taaaaatatt gctattttca tatagttcct cctaatataa atattactcc
     3241 tatttttata tccattaaat atccatatca tttattttat gatacaacaa taaagctgtc
     3301 ttttactaga aaaaatccta ttacaagaca gccttatatt atattaaatc tttaataatt
     3361 cgtaaataga aagagcatat atttgagtag cttttataat atcctctact attaagtatt
     3421 cattagcttg gtgttctgtt tctggttttc ctggaaaaat agctccaaat gcaacacaat
     3481 tatctaaagc tcttgcgtat gttgctcctc cagatgaaag tggtgttcca tcaagaccag
     3541 tttcttcttc atatactttt ctaagtgtct taacaagaag agtatcttct ggaacataga
     3601 ttgaatccaa gaaatcatat tcttcataat ttaaattgta tttatctgtc attttcttta
     3661 actcttttac aaaatcatcc tttttgtatg ttactggaat ccttacatct attcctgcaa
     3721 attctttttc gttatcaata gttactctac ctatatttac tgttagttta cctgatacat
     3781 catccttgca attaggaatt atattgtttc cattagcatc ttcaccaata acttctgcta
     3841 aaaacttaat tatattagaa tctataccta tattattaag agctatacaa agtctagcca
     3901 ctgcatttat accagtatca cttgctgctg aatggacact ttttccaatt atacagattt
     3961 tattaccttc aacagtatac tcaaaattta atttatcaag ttcttttttt aatttatcag
     4021 agtattttcc aatatagatt gctttacctg gaactgcatt taaagccttt ccaactgaca
     4081 actctatatc acttttgcta tcacaagtca aatgaacttg taaaagacct ttctctgcat
     4141 ttgttattgg gaatctagaa tctggagtaa atccatagtt tggtatttct tcattatttt
     4201 ctttatattt atttatacat ctccaaagat tttcttcatc agccccaaaa atgaatctta
     4261 tcctcttatt aaagtctaca tttaaatcca taagagcttt cactgcataa attgctgata
     4321 ttgtaggtcc tttatcatct tgtgttcctc ttccatacaa tttaccatct tctaaaacag
     4381 cttcaaatgg agggtaattc cagctttcta aatcaccttc tggaaccaca tcaacgtgac
     4441 caagtatacc tatcattttt tcaccttgtc ctatttctgc atatccataa tatccatctt
     4501 tgtatactgt cttaaatcct aaagatttac ataagtctaa tatacctctt aaagctttat
     4561 ctacattttc accaaatggg caattttcag ttgcttcaga tataacacta ggtattttta
     4621 cactttcttg tattgaagaa atcatttcat cttgtagaga atttactttt tctttaattt
     4681 gctctttcat attatcaccc ttcaattttc atcataatat actaataaat attttgacct
     4741 ttttaaaaaa aaatatgctt attttttcag tttttttata aattcaatcg cttttaaagt
     4801 ccccattttt gaatggtcaa atgtaagtcc accttgaaaa taaacattat aaggaggtct
     4861 tattggagca tctgcactta attctataga agaaccttgt ataaatgctc ctgctgccat
     4921 tatgacttca ctttcgtatc caggcatgtc ccaaggaaca ggttttacat aagaatcgac
     4981 tggtgcagct tcttgtatac cttcacaaaa acttattact tcatcagcat tattaagtct
     5041 aatacattga ataatatcac ttcttaagtc atcatatttt ggtaatacat catatcctaa
     5101 tttttcaaaa gctcttgagc aaaagatagc tcccattaca gcctgagata ctatataagg
     5161 cgccaagaaa aaaccctgaa gtacatttct agttgttcca aaagtaagac cacattcttt
     5221 tcctattcca ggagaagtca ttctataaga tataagctca atcaaatctt ttctaccagc
     5281 tatatatcca cctgtcaatg caagcccacc accaggattt tttattaaag agccagccat
     5341 aacatctgct ccaacatcag taggctcttt ggtatctaaa aactcaccat aacaattatc
     5401 aaccattact atagcttctg gtttaacaga ctttattact tctatagctt cttttatatc
     5461 actaattgaa agtgattttc tccaagaata tccttttgac ctttgtatca taacgagttt
     5521 agttctatca tttattttgt ttttaattcc ttctaaatct aagtttccat cctctaaaaa
     5581 atctacatca tcatatgtaa caccatactc tttcaatgaa cctttttctt ctcttatgcc
     5641 tataactccc tctaaagtat catatggtct accagtaaca gataaaattt catctcctgg
     5701 tctaactata ccttgtatac ataaagtaag tgcatgtgta ccatttacaa ttattggtct
     5761 tacaagagca tcttctgtat taaatacttt agaataaatt tcctctattt tttcacgacc
     5821 aatatcatta tatccatatc ctgtagtcca gttaaagtgc atatcactta gtttagattc
     5881 ttgcatagct tttaaaactt tatattgatt atattctctt atttctttta tttcttcaaa
     5941 cttatctttt atttcttcca ttatttcttg cgatagttta aaagtatcat catctattcc
     6001 atagtagtct tttaataatt cttttgtctc attaagcatt ttatcacctg tctctttatt
     6061 attttattta aacatgtaaa ggagactatt ttactaaaac aaataaatta tagtttataa
     6121 gttagtataa gtctcctctc aatcatctta aattaaatta attatctatc tgttgttatt
     6181 attattgttg ttttggtttt gatttgttag attaatatac tttccaggtt gtatagttga
     6241 tacagcatgt ttataaatca tgttttgttg tctattatcc ccttctatta atattatata
     6301 actatcaaac ccctttacaa gccctttaac ttgcactcca ttaactaaat atatagtaac
     6361 aggtatcctt tctttccttg cattatttaa aaacaaatct tgtaaattta aaactgtatt
     6421 tttcattaga tttgtaccct cccaagatta aattgaactt ctaaaatatt cctaccttat
     6481 attctatata agttatgaca aattttcaat attaagttgt atacttaaaa atttatatat
     6541 tattttatag aatctttaat atataagatt atttcatttt ttaattcgtc tatattttca
     6601 tattggtcta aatcaaacca tcttgctgtt ttatatcttt taaaccatgt tatctgacgt
     6661 tttgcatacc ttctagaatc tcttttaatt atttcaatag cttcttcgta tgtatattct
     6721 ccatccagat acttcaatat ctctttataa cctatacctt gcattgatat catatctttt
     6781 ttaaatccca tacttaaaag tttttttacc tcttcaacca atccattttt aatcataata
     6841 tcaactctca tatttattct ttgatacaaa tgttctctat ctctatttag aactattatt
     6901 attggttgat attcttcatt aaattttaaa tcacttgaaa aatcattcat tttcttacca
     6961 cttagacaaa cttcaagagc tcttatgacc cttttagtat tgtttttatg gattctatta
     7021 gcagattctt catcaagttc ttttaactta ttatgcatgt aatctattcc cttttcagca
     7081 agctgttttt gaagttcttc tcttaattca ttattggcat cagattgtgc aaaatccata
     7141 ttatatatta aagagtttaa ataaagtcct gtgcctccag ttactaaaac acattttccc
     7201 ttttcattta tttcatgtat atattttttt gctctttgtt gaaactctga aactgaaaat
     7261 ggatagtcag gcttaacttc atctataagg taatgcttca ctccttgcat ttcttcctct
     7321 gtaacttttg cacttccaat atccatatat tcatatattt gcatagaatc agctgaaatt
     7381 atctctgcat ctatatcttt tgctaatttt attgacaaat ctgtttttcc aacagcagtt
     7441 ggacctgtaa gtattataag tggtattttt ttcataacat tcctttccat atattttaaa
     7501 tatccaaata ttttacataa ttcttttaaa cattttttct atttctgtct tagatatttc
     7561 gaccattata ggtcttccat gagggcatgt aaatggattc tcacactttt ctagctgttc
     7621 caataaactt tttatttcaa tatcgtatat tttatcattt gcttttatag cagacctaca
     7681 tgccatagat gcaaatctct ctccttttaa atcataatta cttgtaattt cttctatatt
     7741 atcaattatt tgaagtataa atttctcagt ttcaggtact ccaaatatag taggaacaca
     7801 tcttaccatt atatgattat tgccaaaaat ttctagttca aaaccaaatt tcataaataa
     7861 ctccaagtta ttttcaattt gaagcatatc tacatttgat acttcaataa ccactgggtc
     7921 taaaagtatt tgcatattga tatcttgtct atagaacttc tccatatatc tttcatatag
     7981 tattctttca tgtgcagcat gttggtccaa caaatacata gaatcgtctt tgctcaaaat
     8041 tatataagta ttaaatacaa ctccaattac agaatatcca tataaggaaa acttgcgttt
     8101 tgaactacac aagtattctt cttcactgtc cttaatacta tctcctaagt aataatttcc
     8161 ttcattttta attccatcaa cttgaaactc ttcttgtata ttatcatttg cactatcttc
     8221 acttagataa ttaatgtttt ttacatcatc ttctacactt gcatttagga cttcacttac
     8281 tgattgaaat tcatttattg gtttttcact tgataaagtc acaacttcaa tgacttcatc
     8341 cttatcttta gtaatattac tatttttagg agtagattct aataaatcat tatttcttaa
     8401 tttataatct gtactttttt ctctactgtt aatagcgatt ctaggttgtg tttttttatc
     8461 actataagta gcatattttc caataagatt tgagtgtatt aatttaacct ttaaaaaatc
     8521 tctaagttct atatacactt cttgttcttt ttcaaattta atttcaagtt tattggggtg
     8581 tatatttaca tctatacatg atgggtctac ttctatattt aagaagcaca ctgcatgttt
     8641 acctattgga attatagact tataactttc tgttattgca tctatgatta ttttactttt
     8701 tacaaatctt ttatttatat agatatgttg taagttttta tttgaacggt atatattgtt
     8761 attacctata tatccattca ttttaaagtg attgcactta aactctacat ctattatatt
     8821 ttccgttatt tcttttccat atatacttct aatagtgttt acaagttttc catcccctgg
     8881 agtatttaac atctgtttat tgttatttgt atatttaaat tgaatgtttg gattacctat
     8941 tgcaagttta tttatcaaat cacttatgtt tatagtttct gcatgagtag attttaaaaa
     9001 tttctgtctt gctggtgtat taaagaaaat atcttttata atgatggtag ttccattagt
     9061 agaacctatt ggttcttttg atattatttt tccgccttca acatatattt tagttcctat
     9121 tatttcatct ttagttttag tcgtcatttc taattttgat acagctgaaa tagatgccaa
     9181 tgcttctcct ctaaaaccaa gagagtataa atcatacaaa tcatcaattt ttttaatttt
     9241 actagtagca tgtctcaaaa atgatttttc aacttcactt gaaggtattc ctattccatt
     9301 atctgtaatc ttaattagac tttttcctcc atcaattata tctatggaaa ttttatttgc
     9361 acctgcatca atggagtttt caataagttc ttttactaca gaagaaggtc tttctacaac
     9421 ttcacctgct gctatcttat ttatagtcaa atcatctagg atgttaatta tatttttcat
     9481 ttttacacct ctaagatttt atttcttttg cttttctttg aagattataa agagagttta
     9541 tagcatctaa tggagtcata tttaaaatat ccagatttaa aatttcttct gatagtatat
     9601 ctctatttac actatcaaaa gagatttgag ttaaggctac ttcttttaca gaatcttctt
     9661 gattaatttt ctctttatca tcattttgct tcattaaagc aacttcatca tttaatgctt
     9721 taaatttaga atttaattta tcataatcct tttttatatg tttgtagtct tttactataa
     9781 gttcatgttc tatttttaga gattcatatt tattcttaaa attactttga tttaccttaa
     9841 ctaattcttc gtccaaatta ctattaatta tattattgtt ttctttatct ccatttattg
     9901 caacactgtt gtaaacatga tttttttcca agtctttaag tatatatttt gctctttcta
     9961 tgacttcgtc tggtaattta gctaatttag caacatatat tccataactc ttgtctgctc
    10021 cctgaggaat tatttttctt aaaaatatta ttccttcccc atcttctttg actgctattg
    10081 aataattttt aacctcttta aactcttctt ctaaatcagt aagttcatga taatgtgttg
    10141 caaataatgt cttacatcta atattttttt gaatatattc cactattgac catgctaaac
    10201 ttatcccatc ataggtacta gttccacgac ctatttcatc taatataact aaacttctct
    10261 ctgttgcatt ttttaatata agggatactt cattcatctc taccataaaa gtagattgac
    10321 cttgagataa atcgtcacta gctccaactc ttgtaaatat tctatctaat attggaatat
    10381 cagcagactc tgctggtaca aatgagccta tatgtgccat aagagctatt atagcagttt
    10441 gtctcatata tgttgattta cctgacatat taggaccagt tattatgtta ataatgtttt
    10501 ctccacgatt taaataagta tcatttggaa cgaaattctc ctctcctact atattttcga
    10561 caacaggatg tctaccattt cttatatcaa gtttattatt ttcatttatt gctggtttta
    10621 catagttatt tatatgtgca actgttgcaa gggacacaaa aacatctata ttagctatag
    10681 tcttagccac tttttgtatt ctatctatgt ttttatatat tgtatctctt atctctacaa
    10741 atatctcgta ttctaaagat ttaatctttt cctctgcatg tagtatcttt tcttctattt
    10801 cctttaattc tggagttata tatctttctg cattactaag tgtttgcttt cttatatatg
    10861 tctcatctag ctttgcctgt ttaaagttgg cttttgttat ttctatataa tatccaaaaa
    10921 ctttattaaa tcctattttt aatgatttaa ctcctgtttt ttctctttcc ctgttttcta
    10981 tttcctttac aagaaaggct ccattcttag aaatctctct tagttcctta agttcatcac
    11041 taaaatctga tttaattatg tttccatctt ttatggttat cgtaggttct tctagaatag
    11101 cttcatctat taaattgtat atatcatcta atttatccat ttctgaaaca tactctttta
    11161 ggatttttgc atttgataaa tttatagtat cttttaaatt aggtaacttt tctatcgaat
    11221 tttttaagtg aattaactct tttggtgtta ctctctcaaa tgctatcttt ccacatattc
    11281 tttctatatc gtatatattt ttaagtatat catttaggtc ttctcttaat ataaagtcat
    11341 cttttatttc ttctattaca tctaatctat tttcaatttt agatttattt atcaaaggtt
    11401 cttctacata ttttcttaaa agtctacctc ccattgctgt agaagtttta tcaagtacat
    11461 gtaataatga accttttttc ttattccctc ttatagtttg agtaagttct aaattagttc
    11521 tagtaaacat atctaaaacc atatattctg atgaattgta tatatttata ttattaatgt
    11581 tagatgttat ttgtttttga gtattgtata tataattcag aagaattgat aagcttgact
    11641 ttattagacc tttatcatca aactttagtt tttgtagata aacatctgga aaatattctt
    11701 ttaatatatt tatatccaaa tagttatcac taaaactttc atttatgtat atattactta
    11761 cagtagctat atctctaagc ttttctataa agtctaaatc atttataatt atttcagttg
    11821 gatggatttt tgcaatttct tcaattacct tatcttcatt taaacatgtt gcattagttt
    11881 ctcctgtact tatatcaaca taagttagac ctatattagt accatcctta tacaaagata
    11941 ataaataatt attcttttta ttttcaagta aatttccatc taaaactgta ccaggagtga
    12001 tgactcttat aacctctctt ctaacaattc cttttgctgt agatgggtct tccatttgtt
    12061 cacctattgc tactttatat ccattttcaa ctaattttga tatatatgaa ttggctgagt
    12121 ggaaaggaac accacacata ggagcacgtt cttctaaacc acaagctttt cctgtcaatg
    12181 ctatttcaag agcttttgaa gctactaatg catcttcaaa aaacatttca taaaaatctc
    12241 caagtctaaa aaacagtata cagtctttat atctattttt tacttcaaga tactgtttca
    12301 tcataggagt taatttattc atatctattt gcattatcaa acctccttct tttctaatta
    12361 aatattatat catagtagat gattattatt aacacaatat ataaaatttt gacatataac
    12421 taaaaacaat ccaaaatgta tactcacatt ttggattgct aagtttaatg cttattaaat
    12481 aaaaatatat atatttttac aataaagata ttatttcttt tttaagttta atgatatttt
    12541 caaggttatc ttcatttaac tttctactat ctataacaaa ttctttcttt actattgatg
    12601 gtttttttga aataacataa attctgtttg atagcattat agcttcgtca atatcatgag
    12661 ttataagaag tattgttgaa tttacttttt tcttaacatc taaaagccat ttttgcatgg
    12721 atgttttagt taatgaatca agtgccccaa aaggctcatc taaaagcatt atgtcattag
    12781 aatttataaa agttctcaag aagttagctc tctgtctcat tcctccagaa agttctgaag
    12841 gatatttatc ttcatatccg cttaatccaa acacctcaaa ataaggtttt accattgacc
    12901 tagctttact ctttttttct ttttttaaaa ttagtggtag tgacacatta tctattatag
    12961 ttttatatgg caagagtaaa tctttttgat acatataact atatttacca tttatattca
    13021 ctttgccact atcataatct gtaagtttag ttataatatt aaaaatagta ctttttccac
    13081 atccacttgg ccctaatata gaaacaaact caccttcata aacatcaata cttatatctt
    13141 ttaaaacttc aatatcttta taagtttttg atacattttc tatagatact tttacttttt
    13201 tattgtggta aaaattcatt tgtgtaagca tcacttgcct tcatatcctt atctattaat
    13261 ttatattctt ttaagaatga tgtatagttg ttccatacac tatctttcat ttctccccat
    13321 cgtggtgcat catctttgta cttgcttgct aaatattctt gactctttaa agctagtttt
    13381 tcatctactt caggtgcatg ttttactaaa atttttgctg attcttctgg attttttatt
    13441 gcatattcat aaccttttgt tgtagctttt aagaattttt tagcaagctc tggattatct
    13501 ttaagcacag tttcattaga aattataagt ggtgtataat aatccaatct ctcatctaaa
    13561 tcttttactg gtataaagtt taagtcatat ccaattaaat cagctttaac tgcatcccaa
    13621 ccttcaaata tccatgcaaa atctacagtt ttcatagctg caaagaaatc atcttgacca
    13681 gtattaacta tctttaactt attaaaatca gctccatttt tcttcataac tgctttaaaa
    13741 acagcttctt ctgatggaga gccccaacca ccataagttt ttccttcaaa atctttagct
    13801 gttgttatat tcttttcttt tggtgatgca aacccagaag tattatgttg tataacagtt
    13861 gctacagctt ttattggaag tggatcttca ctagtctttg cataagtaac ttgttcttga
    13921 tagcttatac caaagtcagc ttttcctgtt gctactaaag tttctgctcc actttcaggt
    13981 ggttgtacta tttcaacatc caaaccttct tctttatagt aacccttatc aagtgctaca
    14041 aataatccag tatgatttgt atttggtgtc caatcaagca ccatagttac ttttttagaa
    14101 gaagtatctt tatcattgtt tttgtcttta ttgttatttg aagaacatcc agttattgtt
    14161 gccaatgtaa aaacacttac taaacctaat attcctaact ttttaaaatt cataattgcc
    14221 tccctcaaat acctttaatc aattaatcta tgtcttataa caactttttt tacagcctgt
    14281 acaattccta caaacattaa actaaatata actactaata tagtagatgc aaatacttta
    14341 tcaagtgcat atgcactttt tgacctaagc atataaacac caagtccttt cgttccacct
    14401 agccattctg caacagttgc tgccattaca gcatacgttg cagatatttt aagtccagaa
    14461 aaaaatttat ccattgccat aggaaattta aggtgaatga atgtattaac tttatttgag
    14521 tccatagtct taaataaatt taaataatct ttatcaatat tctcaatgcc atctacaaaa
    14581 cttattaata taggaaaaaa acaagtaagc attaccatca atacttttgg cattgttcca
    14641 aatccaaacc atatcataaa aagtggtgct ataattattg taggtatcat ctgtgtaacc
    14701 aacataatag gataaagaca tttttttatt attgatacaa aatccatcaa tattccaatt
    14761 accaatgata ttataattgc gactataaaa ccaagtattg cttctcctat agtaaccaca
    14821 gcatgcatgg ataaattttg ataatcttta aaaaacacat taattatatc tactggtgtg
    14881 ggtagtatat actgaggaac ttctaaaact cctaccatag attgccaaag aataagtata
    14941 aacaaaaatg ttgctattgg atatatacta tctttgactt tatctatgat ttctaagttt
    15001 ttcatctata gtcagacctc cagctttttc atctactcta gctactgtta tgactctttc
    15061 acatccaaga ttaaaaggga ttttatgtac cttttttaat aattcaaaaa cttcatcaaa
    15121 atcaccttca acagtagtgc tcattgctcc tatttcacat ttaagaccac ttttttcaat
    15181 ttcagcaata caagcatcta ctactttata catttcttct tcacctttgt aaggtcttag
    15241 tggcattact gctatgtctg cgattaccat ttaaatcact cctttaaaaa taattaatac
    15301 ctactaggta tattttgttt tttattaaaa aattttatta ctaaaaagat attatattta
    15361 aataaaagaa aagtcttatc caaaatagga taagacttat ttttatctta actggtctcc
    15421 ctacactggc attatccaaa caggttcaaa gggtcgagat aaaatctcct ctcagcacat
    15481 tagctccccg aaatatatta tatcttttct acttgattat actacactta tcatatttta
    15541 acaaattttt tatttatatt agatatttta tccgttcaaa aatatctaat atacattgta
    15601 cattattaca ccaaataaat tatcttaaaa tggtaattta tcaacatctt ccaaaactag
    15661 attaactgaa ttttctagtt tttattttgc tatttcaagc atatttggat gtatatcaat
    15721 tccatacaaa tattgttcta aatttctgtt ttcactaatc taatttgcaa tcgttacaga
    15781 ttacagatta gaaatagctt ttagactcta cttatcttta tctacaaaaa atccttcaat
    15841 aaattcagta ataatattca gtaatctaaa aataaacctc tatctggatt tttaagatac
    15901 aagcctttta ggaagtatct tttgtgataa tataaaaatt actcgtcatt atcatcaaaa
    15961 tcctcagcaa ccaacatttt agataagaat caaactctat aatataatta ttttatccaa
    16021 tttatttttg agaaataaaa tattttttta atttttaaat tttttaatat gcaccttgaa
    16081 aaaagaaacg ttttcctata ttatggtaat agtagaataa aactaaatag gcaaaactag
    16141 agaaatttag tgacgcaaag ctatagggac taagacttat gaaatgagtt atgtcagcca
    16201 gttgccaaag agatattttc tttttgtttt tatgaatgtt ttttaggggg atatatgata
    16261 tatgtttatg gataaaatgg atagatgtac acatatacta actgcatata tatgcagttc
    16321 acgcgattac tgcaatttta tagatacaca attaaatgat tttatactag aatatggaga
    16381 gaatgtggta gaatcttgtt tgcatcaagt gatggtattg gtaagcagat ataattaaag
    16441 aaattttatt ttctagaaaa ccctttttag ttttatctac gttttataat tcattaaaga
    16501 actaatcaaa ataagcttca ttatatattt ttcatgaagt aaacagttga gtttatctca
    16561 tatactgttt accccaatta cataattcta ataatataga ttccaatgat ttaccacgtt
    16621 tagtagacaa atattcaact tttggaggaa tagtcggata agaaattctc ttaataagtc
    16681 catctctttc aagatacttt aattcttgtg acagtatctt atgacttata tgaacaacat
    16741 ctttttttaa ttgactgtaa cgtattggct cttgttttaa aatatgccat aaaataattg
    16801 ccctccattt acttcctatc acagtcatgg taaaaccaat tgaacaatta aaatcttttt
    16861 gtctttcttc aatatttgga aattcttcaa aattattatc tatatccatc tcaatcctct
    16921 ctttcaaaat agtaagtatc taactaaaaa gtgcattctt gtttagcgta ctaattcgta
    16981 atataatctt gattaaaaaa tagctacgca cactcgtgac tttagtcatg agttagtagc
    17041 taaaatagtc aacatatagg gaaac
//
LOCUS       DAMJVX010000074        17041 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMJVX010000074 DAMJVX010000000
VERSION     DAMJVX010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17041
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.077"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            100..867
                     /locus_tag="PC407_002131"
     CDS             100..867
                     /locus_tag="PC407_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2497576.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            1150..1347
                     /locus_tag="PC407_002132"
     CDS             1150..1347
                     /locus_tag="PC407_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2497577.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            1376..2452
                     /gene="vorB"
                     /locus_tag="PC407_002133"
     CDS             1376..2452
                     /gene="vorB"
                     /locus_tag="PC407_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2497578.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            2455..3174
                     /locus_tag="PC407_002134"
     CDS             2455..3174
                     /locus_tag="PC407_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2497579.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            3187..3726
                     /locus_tag="PC407_002135"
     CDS             3187..3726
                     /locus_tag="PC407_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2497580.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            3883..4941
                     /gene="buk"
                     /locus_tag="PC407_002136"
     CDS             3883..4941
                     /gene="buk"
                     /locus_tag="PC407_002136"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2497581.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            4959..5852
                     /locus_tag="PC407_002137"
     CDS             4959..5852
                     /locus_tag="PC407_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2497582.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            5882..7090
                     /locus_tag="PC407_002138"
     CDS             5882..7090
                     /locus_tag="PC407_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2497583.1"
                     /translation="MKYAKRMDMVKASAIRASQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDIVVTENPSYLGAINAFRPYECDFLGVDTDDEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            7114..8376
                     /locus_tag="PC407_002139"
     CDS             7114..8376
                     /locus_tag="PC407_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2497584.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFSTPVLGMRWVMISLGILSIVMCPVLWFVIKDVDIREDKEEEASEKMSAGDFM
                     TVLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFG
                     APFGGYCADKVKSPTKVLLPIYVFGIAVIILFLVLPASTPMTIFIALTFVVGILGYMG
                     KGIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIF
                     QIAILIIGILSCLYILKVKKKRESKLEA"
     gene            8952..10415
                     /locus_tag="PC407_002140"
     CDS             8952..10415
                     /locus_tag="PC407_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2497585.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSMKLRFFQIKDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSILFSVVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVTSSIALGIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIIANINLVPGAIMTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIIITGMYNVTDVYTTLPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKHIIN
                     VCRFMLIICAAMCCFIEPLTSWALGDICIGVLTYINLVGIFTLKKPAIKAYYDYQRQK
                     KMGVKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            10471..11607
                     /locus_tag="PC407_002141"
     CDS             10471..11607
                     /locus_tag="PC407_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2497586.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            11843..12958
                     /locus_tag="PC407_002142"
     CDS             11843..12958
                     /locus_tag="PC407_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDF2497587.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALTPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKAKKIGEKELMNLLSLKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            13110..13979
                     /locus_tag="PC407_002143"
     CDS             13110..13979
                     /locus_tag="PC407_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2497588.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
     gene            14171..15061
                     /locus_tag="PC407_002144"
     CDS             14171..15061
                     /locus_tag="PC407_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2497589.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLSPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            15279..17033
                     /locus_tag="PC407_002145"
     CDS             15279..17033
                     /locus_tag="PC407_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HDF2497590.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDVTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNVKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVSVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLVGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
BASE COUNT         6657 a         1822 c         3274 g         5288 t
ORIGIN      
        1 aatataatta agataaatag ataattttgt aattttaata tataatattt agtagagtgt
       61 gggagtagca cttaatattt gcaggaggag tttttgtata tgggagaaat aataaatgca
      121 ttagatagag ccctagacat aattttgctg ttatatcatg aaaagagaga aatgggaata
      181 actgaaataa gtaaggctat gggagtatat aaaagtactg tacatagaac tttggttact
      241 cttgaaaaca aaggttttgt tatacaaaat gcagagaatg gtaagtattg gctaggaata
      301 aatttatatg caataggtat ggttgtggga gaaaaaatgt cattgactga aatagtaaaa
      361 ccatatacga aaaaattaaa tcaagaattt aacgaagtag ttaatgtatc tatattagaa
      421 gaaagagctc aagattctcc aagaagtatc ataatacata aagagtatgg ttcaaatcaa
      481 ttactttcag taaatccatc cgttggttca agtagtgaat gttattgttc agcagttggt
      541 aaatgtctta tggcatttaa tgattctata gattttgaaa aatatagaaa aacaccaata
      601 cacaagtata cagaacatac aattgataat tgggatgata tgatgctttt tttagcaaaa
      661 ataaaagaac aaggatatgc tatagatgat gaagaactag aacatggact aacttgtatt
      721 ggagcaccta tcttagataa aaataataaa gctattgcag ccataagttt gtcaggtcca
      781 actataagaa tgagagaagg ggactttgag tataagatta aaagagttat agaaactgca
      841 aaaagtatat ctgaactttt tagatgattt ttaatctcga taataaaaat aggctatatt
      901 taaattgtta tttgtaaata tcagtttaaa tgtagcctat ttttctttaa tctaaaatta
      961 aatattctta aaatttaaaa tgttattgaa atatataagc gaatgtgtta tcttttaatt
     1021 aagcaataaa aatagaacaa ggttctatac tatagaacaa aattgatgca tatatagtat
     1081 ttatacaaaa tataaatatt atatatttaa aattaagtaa aaatattaca taaaggggag
     1141 atttgagaaa tggaaaaagt caagttgagt gttgaaagat gtaagggatg ctatctttgt
     1201 attgaagctt gcaaaaaaga tgcaataagt ctttctgact acacaaataa aaaaggcttt
     1261 gtaactatta aagtcgatga agaaaaatgt attcaatgtg gcgcttgtta tacaatgtgc
     1321 cctgatttag tatttgagat attataaatt atttttatta gaggggagaa aaggtatgca
     1381 aagtttatta attaaaggaa atgaagcttt agcagaaggt gctatacgtg gtggctgcag
     1441 attttatgca ggttatccaa taacaccaca atcagaaata ttagaaatga tggcatggag
     1501 acaaaaagaa gttggaggag tatttatcca aggtgaatca gaaatagcca gtgtaaatat
     1561 ggttatggga gcaagtgcac ttggagcaag agctatgact tcttcatctg gtccaggatt
     1621 ttcattaaaa caagaaggta ttgcatattg ggtatcagct ggtttgccag cagttgtagc
     1681 atgtgtgcaa agatatggta taggtgatgg atttatagga gctggtcaag ataattattg
     1741 gcaagcagta aaaggcggtg gaaatggaga ttatcattta atcgtgttag cgcctaacag
     1801 tgttcaagaa aatatggata tggcatatga atcttttgaa ttggctgaaa aatatatgca
     1861 tccagtactt atactttctg atgctacaat aggtcaaatg gtagaacctt gtatagcacc
     1921 accaatgaaa gaatacgata tggataaagc tccatgggct gcaaaaggaa cacctgtagg
     1981 agaaaaaaat aaagtaataa ctgatataac atattttgaa gaccaagata catggcaaaa
     2041 agggtatata gaaaaaatga gaaaagttca agaagatgaa caaagatggg aagagataga
     2101 aatagaagat gctgaagtgg tatttgtggc atacggtatt tcatcaagaa tagcagaagg
     2161 tgcagttaga gatgctagag caaaaggaat gaaattaggt ctaataagac caaaaacatt
     2221 gtggccttac ccaagaaaag catttaagaa attaagtgat tcattgaagg gacttgtaac
     2281 agttgaaatg aacatgatgg cacagatgaa ggaagatgta attatagcat gtgataatag
     2341 atttccagta tattcagtag caacagctca atatatggca gaagtaaatc aattaataga
     2401 ttttgctcaa cagattatag atggaaaagc taagcaagag gaggtatttt aattatggct
     2461 aagaaagcac cagcaataat gatgtgtgaa aatatgtttt gtggaggctg tggacactct
     2521 atagtcgata gaattattgg agaagtatta acagaaatgg atatatgtca agatacaatt
     2581 atatgttcag atataggttg taatcatatg tttcaattta gtatgaatgt agatgttgtc
     2641 gttccacctc atggtaaaat gggtgcagca atggcagcta tgaaaagagt tagaccagat
     2701 aaatatatat ttacaatagc tggagatggt ggagcttatg cgatagggct tggggaaact
     2761 atgtcagctg ctactagaaa tgataatgtt atattccttg ttatgaacaa taatatgttt
     2821 gcaatgacag gagggcaaat ggctccaact actatagaag gtcaatatac agcttctact
     2881 ccagcgggta gaaattacgc agatcatggt tcaacatttg atgttgtaaa agttatggga
     2941 aatttagata tagcatatct agcaagagga acaataacta accctaaaga aataaataca
     3001 acaaaaagat acatcaaaaa agctctagaa aaacaaaaag aaaagaaagg tttttgctta
     3061 atagaagtgc ttgtaccatg tccaacaaac tggggattaa agccagtaga tgctatgaag
     3121 agaattgaaa atgaacttgt tccaaagtat ccacttggag aatttattga ttagggagga
     3181 aataatatgt tgaatgaaat tatatgtgct ggttttggag gacagggagt tcttacagca
     3241 ggaaaaatta taatgtatgt tgcatataag gcagaaaaga aaattacatg gttcccatct
     3301 tatggaaatg agatgagggg aggaagtgca aattgcaatg ttgttgtaag tgatgaaaaa
     3361 attgcaaatc cttgtgtaaa aaatccagat atactattgg gaatgaacag cgtatcagta
     3421 gataagtatg aaagtttaat gaaaaaaaat ggttatatgt ttgtcaatga atcactagta
     3481 agtaaggata gaaagtacag agaagacatt aatgttataa aagtacctgt tactgaaata
     3541 gcacaaaatt taggcaatga aaaagctgct aacatttgca tgttaggtgc tatggtaaag
     3601 agtttggatt tatttacaaa agaagaattt gaaagtggta tgtgtgaata ctttgaaaat
     3661 caaggcaaag gtaaattcaa ttctaaaaat gtagaagcat ttgaagcagg atacgactac
     3721 gaatgaatat tttaaatatt taaaataatt attgaaatac ttatttttat atgttagtat
     3781 ttaattaata aaatatagaa cgtcgttctg gacaatagga cgaagtcttt aggccggaat
     3841 acaagaacga cgttctgtat agtagaacta gggggtgtta aattgaaagt tttagtaata
     3901 aatcctggtg gtacatccac aaaaatttct gttttccaag atgaaaatga aattttgaaa
     3961 aaaaatataa cccatacaag agaagattta aaaaattttt ctaaagtatt tgatgaatat
     4021 gattatagaa aacaactgat agtagatatt ttaagtagtg agaatcatag tataaattct
     4081 tttgacgcag tagttggtcg tggtggactt atgaaggcta taaaaggtgg tacctatact
     4141 gtgagtgaag aaatgattga ggatatgaga aatgaaataa atggggaaca tgcatcaaat
     4201 ttaggtgcat tattagctaa aactatagct gatgaaatag gagtacaatc atttgtagta
     4261 gacccagttt ctgtagatga atttgatgat gtatcgagga ttacaggaat atcagacata
     4321 gaaaaagcaa gttggttaca tgcattaaac cataaagctg tttcaagaaa aattgcacaa
     4381 aaactagggg gaaaatacga ggattataac tttattgtag ctcatttagg ttcaggaata
     4441 tcgattgtag cacataaaca aggcaaaatg attgatggaa gtggtggtag gtctgatggt
     4501 ccattttctc cagaaaggag tggtggatta cttgcatatc ctcttataga attgtgttac
     4561 tccggaaaat atactcatga tgaaatggtc gcaaaagtaa gttcgattgg gggaatgtac
     4621 gattatttag gaacaaagga catgatagaa attgaaaaca gaatcaagga gggtgatgaa
     4681 aaggcaaaac ttataatgga tgcatttatt tatcaagtag caaaagaaat ttctatgtat
     4741 ggagcttcac tttcagggaa tgtagataga gttatcttga caggagggat atctcactca
     4801 gaaatggtag tcaaaggagt agcagaaaaa gtttcatatc tagctccaat agagatagta
     4861 ccaggagaga tggaaatgga ggcacttgct ctaggagcac ttagagtact taatggagaa
     4921 gaaatagcaa aaacttatta atatttagga gaaagagtat gttaaaagat tttaataatt
     4981 tattagaaga ggctaaaaag cttgaacctg ttattgtaag tgttgctgtt gcagaagaca
     5041 aagaagtatt aaaggctgtg aaatcagcaa cagatttaaa cattataaag ccaattcttg
     5101 taggagacaa ggataaaata gaaagtatat caagagaaat tggattaaaa ggtcatgaaa
     5161 taataggttg tcaaaatccc gatgaatcag ttaaaaaagc tgtagagata gttaaaaata
     5221 aggaagctaa agttcttatg aagggattag taaatacaag tgtctatatg agagctatat
     5281 taaataaaga aacgggtctt agaacaggaa gattattaag tttgcttgca gtatatgaat
     5341 tacctcaata tcataaactt ctttattgta ctgatagtgg aataaatgta tcgccaaact
     5401 taaatcaaaa aaaagatata atgacaaatg cacttcttac tatgaaaagt ataggcatgg
     5461 aaaatcctaa aactgcgata cttactgcaa atgaaatggt agaccctaag atacaatcta
     5521 cagtggatgc caacctactt gtagagatgg aaaaatcggg agaaatacca aagtgtatag
     5581 tggaaggtcc aatagcattt gatgtagcat ttgatgcaca tgcagcagaa cataaaggta
     5641 ttaaaagtaa tattgctggt gatgttgatt tattagtatt tccaaatata gaagcaggga
     5701 atatattagg aaagtcatgg cttagattta ataaagctaa gtgggcaggt atagttttag
     5761 gagcaacaaa tccgataatt ttaggttcac gttctgacac agctgaaata aaaataaatt
     5821 caatagcatt agcgtgccta gcatcacaat aagaaattat tattaaggag gagaaagtat
     5881 tatgaaatat gcaaaaagaa tggatatggt aaaagcttca gctataagag cttctcaaaa
     5941 gaaaatagct cagaaggtgg caagtggtgg aactgttata tcttttgctg cagggcttcc
     6001 tgacccaaat ttatttccaa tagaagagat aagtgaaata actcaaaaag tacttaatga
     6061 aaggggaaaa tttgccctag catatggacc tactaaaggt gaagatgagt tgttagatct
     6121 attagtaaaa agaatgaaag aagaagaaaa tattgataca aaagcagaaa atattataat
     6181 aactactggg tcacaacaag ggatagcatt aagtgctatg attttactag aaaaaggaga
     6241 tatagtagtt acagaaaatc caagttactt aggtgcaata aatgcattta gaccttatga
     6301 atgtgatttt ttaggagtag atactgatga tgaaggaatg gtaacagatg atttagataa
     6361 attattatct gaaaattcta acataaaggt aatctatgta ataccaacat ttcaaaatcc
     6421 tacaggaaaa acatggtctt tacaaagaag aaaagatttt atggaagtcg tgaataagta
     6481 tgatgtcata gtaatagaag ataatcctta tggagaaata agatttacac cagagcctct
     6541 acctacactt aaatctcttg atactaaaga taaagtatta tacttaggtt cattttcaaa
     6601 agtattatgt ccagggttta gagttgcttg gatgtgtggt aatgtagaca tcatagataa
     6661 ggcagaactt ttaaaacaag gtatagattt acagtcaaat caattttcgc aagtacaagt
     6721 aatagaattt ttaaagaaat acaacttaaa tgaacatata gaaaaaataa gagcagagta
     6781 taagaaaaga tgtctgctaa tgttagatgc tatgaagaaa catttcccag aggaagctaa
     6841 atatacagaa ccagatggag gaatgtttat atgggttgaa cttcctgaaa gtataaatgt
     6901 agatgattta ttggataaag ccattgatgc tggagttgct tatgtttctg gagagtcctt
     6961 ctttgcaaat aatggtccaa aaaatactat gagacttaac tatacaacaa tgtcagaaga
     7021 acaaatcgtt aaaggtgttg agatacttgc agaagttata aaaaaagaac tcaattatgc
     7081 ttgtgaataa atataaaaag tgagggttta aatatgggag aaaaagaaaa cggtataaag
     7141 tttggaaagt taatgtttca acttgttatt atagcaatat cttgggaatt agtttatatt
     7201 ataccattta tccaatatac attatatgat ccaatcctaa aagcattaga atgtacaaac
     7261 actcaactag gttttctact tacaatttat gggttaggta atatatttgg agctcctata
     7321 ggaggctggt tagcagatag atttgattat agaaagataa ttttaggttc tgtatttctt
     7381 aatggagtag tatcattttt atttgcattt aatatgaact atccatttgc agttgctact
     7441 tgggtaggtt gtgcaatcac atcattagtt atgaactatc cttctatggt aaaaatactt
     7501 agagttatag gaaaagataa tcaaggtaaa gtttatggat tcaatgaagc tatggttgga
     7561 gtttctggcg tagtaatggg agcaatattc ttatatattt atacatgctt ttcaactcct
     7621 gtattgggta tgagatgggt aatgatatct ttaggaatat tatcaatagt gatgtgtcca
     7681 gtattatggt ttgtaattaa agatgtagat atcagagaag acaaagaaga agaagcaagt
     7741 gaaaaaatgt ctgctggcga ttttatgaca gtactaaaat caccaaacac ttggctagta
     7801 ggaataagca tattctgtgt atattcattc acagttacaa tgtcttattt tacaccatat
     7861 ataacatcag tacttggagg ttctgtggca ttgtctgggg cattagcaat aataagacaa
     7921 catggtctaa aattgtttgg agcaccattt ggaggatatt gtgcagataa agtaaaatca
     7981 cctacaaaag tactactacc aatatatgta tttggtatag cagtaataat tttattccta
     8041 gtactacctg catctactcc aatgactatc tttatagctt taacatttgt agtaggtata
     8101 ttaggttaca tgggaaaagg tatatactat gcagttcaag atgaagtaaa agttccagtt
     8161 aaatattcag caactactat aggtatagct gcagctttag gattttcacc agatgtattc
     8221 caatttgcat taataggaca ctggatagat acttatggca ataaaggtta cacatataca
     8281 tttatattcc agatagcaat tctaataatt ggtatactat cttgtttata tatattaaaa
     8341 gttaagaaga aaagagaaag taagttagaa gcataaatat tgtaaaaata ccaaattatt
     8401 aagttttata gcaatactta aaatttaaag tgcaacacac caaaatgtta tcaagttttc
     8461 taggttttag tataaagtta cccctaaagg tctggaattg agtagcaatt atcatctatt
     8521 tatatattta cataaaccta tttaatttaa attaaaagaa tttcaaaaat aggtggtaat
     8581 tgtactcaat tttacacctc ataataaata cgaaaattta aaatctaact atatactatt
     8641 caattatata ttaataattg tatatggtac tatttatata gtaacagttt caggcttttc
     8701 attacatgta ttccaattta tattgtttga ttatttgtca aatacatatc gttaaaaagt
     8761 ttataaattt acgtttatat atcgattaat attctttgtt ttagagatag atacttatag
     8821 cttgtggatt aaaaataata taaatagaag atagattttt tagatatatt gagttgaaga
     8881 ttatatagaa tgttgaaaca tagctattta aattattgtc tataaatata aaatgaatgg
     8941 tggtgagtta agtgaccgat ttagttaaca ccattaatgg aattgtttgg tcacctgtat
     9001 tagttgcaat gtgtttgtgt ataggattat atttttcaat gaaattaaga ttttttcaaa
     9061 taaaagatgt aaaagaaatg tttactcttt tgcttgaagg agatagctct catgttggaa
     9121 tatcttcgtt ccaaggtttt gctacatcta tggcaggaag gataggaact ggaaatatag
     9181 ctggtgtagc tgtagcgata gcaatgggag ggccaggagc attagtgtgg atgtgcataa
     9241 tggctacatt agggtcggca actgcatttg tagaatctac tttagctcag atatataaag
     9301 atgagcatga tggacagtac aggggaggtc ctccatatta ctttgaaaaa ggtcttggtt
     9361 ggaaaaaatg ttcaatttta ttttcagtgg ttgcaatcat aagttataca ttttttatgc
     9421 ctggcacaca atcaaatact ctatatctag caatgaatca agcatttgga ttttcaaaaa
     9481 tgacaatagc agttacatct tctattgcac taggtataat aatttttgga ggaattaaaa
     9541 gaattggttt atttgctgag aaagtagtac ctataatggg tggagcatac cttattatga
     9601 cattggtaat aatcatagca aatataaatc tagttccagg agctattatg acaatgttta
     9661 aatctgcatt tggatttggt tcggtttttg gagggatgtt aggttcagca atatcttggg
     9721 gagtaaaaag aggtatatac tcaaatgaag cgggagaagg ttctggaact tatggtgcag
     9781 cagcagcaga agtgagtcac ccagccaaac aaggattagt ccaagctttt tctgtttatg
     9841 tagacacact attaatatgt atggctacag gtattatgat tataataaca ggaatgtata
     9901 atgttacaga tgtttacaca acattaccag gtgtagaacc aggtccacaa tatgtacaga
     9961 gtgcaataaa tacattacat cctcaattag gtgatatttt tatagcaata gttttactac
    10021 tatttacatt tacaacatta ttagccttct catacatgat ggagaccaac gtatcttact
    10081 tagtgactag aattaaggca gaaaaacatg aaaagcacat aattaatgtc tgtagattta
    10141 tgcttataat atgtgcagca atgtgttgct ttatagaacc acttacaagt tgggcacttg
    10201 gtgatatatg tataggtgta ttaacatata taaacttagt tggtatattt acactaaaaa
    10261 aaccagctat aaaagcatat tacgattatc aaaggcagaa aaagatggga gtaaaaagga
    10321 gcgatagaac ttttaatcct atagaattag atataagaaa tgctgagttt tgggaaaata
    10381 gatacttaaa agaggataag catgagttaa attaaagtta taataaaata taaaaattac
    10441 taaataaatt tagtgggagg taagattggt atgaaagaaa gaataataca agtggctgat
    10501 tcaaagaaag aaaaaattct agggctatgt caatctttat atgatgaacc agaaattgct
    10561 ttgcaagaat ataaatctgc taaaaaaata tcagaatttc taagagaaga gggttttgat
    10621 gttgaggaaa acttggcagg tatggctaca gcttttaaag caaccaagaa aaatggagat
    10681 ggtcctaaaa tagcatttat agcagaatat gatgctcttc caggcaatgg acatgcatgt
    10741 ggacatcatt taattgcatc tatgggagtt ggagctggaa tagctctttc aagtatactg
    10801 gatacttaca agggagaagt aagtataatt ggaacacctg ctgaagaaac tggagatgga
    10861 aagccttact tgatagaaca tggtgtattt gatggctatg atgcagcaat gatgatacat
    10921 ccaaattcaa aaacttgtgt aacacctgaa attatagcaa taggaggtct agatttcata
    10981 tttactggaa aagcatctca tgcaggagct aagccatata acggaataaa tgctttagat
    11041 gcagtagtac tactatataa taatataaat gcactaagac aacaattagt agatggaacg
    11101 agaatacatg gtattatact agaagcagga actgctgcaa atgtaatacc tgatatggga
    11161 aaagtaagat tagagataag ggcaaaagaa caaaattact ttgatgaagt agtagaaaaa
    11221 gtgaaaaatt gtgctagagg tgctgcaata gccactggat gtgaacttga attttatcac
    11281 tttgaaccaa cttgccaagg attaaatgaa aataaagttt tggtagacat atttacaaag
    11341 attatggagg aatttggaat atatgaagat gaacaagtaa tgttaggttc aacagatatg
    11401 ggaaatttaa gtcaaataat gccatgtata catcctttga tgaagttttc tgaaaatgga
    11461 gaagaactac atacaaaaga atttttggag gcttctataa attcatatgc aaaagataga
    11521 gtaattgatg gtataaaaat attagcttta acaggattta atttatttga aaatccagag
    11581 ttattgaaaa aaatgaagga agagtagaac ttagactatt tttaattata ctctttaaaa
    11641 taagaagagt gtctcaaaat gaacttttta gtttatgaga cactcttttt tgcatgaaat
    11701 taaatatatt tttattattt taacaatgaa aaagaggctt atctgttttt atataacctt
    11761 ttttaacttg tagcacaaaa tatcatgaat gattacaatt cgaatgataa aatcgattat
    11821 aagatatata ggaggggtac atatggaaac tttagaagta agaaatattg aattgagtgg
    11881 tacaaattat gagataggat acagactagg agaattggtt gctaatatgc cagagataat
    11941 agaaggtcag attaataaat ctaatctggt aagcaaaaaa gaagaaaaag agatgataga
    12001 gttgtttgat aaatattgtc caggtcttaa tgaagaattg caaggttttg ctgatgcaat
    12061 tcaagtgaat tgtaatcaaa ttttatatta tactatgact tatcttaaac caggttgtag
    12121 tcaagttgca ttaacaccag aattaactga aaatgggcat gtactatttg cacgtaattt
    12181 cgatttctca cacaatatgg aggattttgt tttatgtaaa actaaagtaa atggaaaata
    12241 tgcacatata ggtactaccg ttatgcaatt tggaagaggt gaaggtatga atgaatgtgg
    12301 attaggtgta agtcagtcat catgtggtat tcctgtaggc aattcagatg gtttgagaaa
    12361 accagcaatt gtaggtttac agttttgggc tgttattaga tatttgttag aaaattgcaa
    12421 agatgtagat gaagcattag aatatttaaa agatatgcca attgcataca atattaattt
    12481 attgcttgca gataagtctg gaaacattgc acttgtggag actttagatg ggaaaaaaga
    12541 agtaaatata atcaacagtt tagaaagtaa gagagaatat tttttacact ctactaatca
    12601 tacacatatt gataaacttc ataaattaga accacaatca atgaaaaatt ctattcatag
    12661 atataaatta attaaagaat atataaataa ggctaaaaaa attggagaga aagagttaat
    12721 gaatttattg tctttaaagt atccaaatgg cttatcttgt aattactata atgatttttt
    12781 tggaacttta aaaagtatag ttatggattt aaatatagga aagtttaata ttttatgggg
    12841 tggactagag aataaatggg agagctacta tttaaaaaat gacattaaat ctataacaca
    12901 gaggataaac ataaatattg aaaaagcacc atctgacttt tttgatttca ttgaataaaa
    12961 aatgtattta attattctaa ataattaggc tatataaatc aactaaaagt ttttatttat
    13021 cgtattgaat tagttataat attttagtat agtaaaaaat agtgctattt tacatattta
    13081 aagagtttaa gtagaagaga ggtgccatat tggattactt aaagtatttt gaaaaagctg
    13141 taatgtatat tgagaataat ttacatgaaa atataactgt aaatgatgtg gcaaaagaaa
    13201 ctggatattc ttattatcat ttaacacgct tatttaagtc tatgtttggt gagagtgtag
    13261 gaagttatat aaaaaaaaga cgattagtaa gtagtacaaa agaactttta tatagtgata
    13321 aaaaagtgat agatatagct attggtagtg gatttgaatc ttcggaagca tttagcagag
    13381 catttaaatc tatatacaag gttagcccta tagaatatag aaaaaataga attgatgtat
    13441 ttgttggtaa aaagaaaaaa ttagagttag attttatgag acatcttgtt ggaaatataa
    13501 cgattaaacc agttataaaa gaaatagaaa aaataaaggt aataggtata aaggataaag
    13561 taattttaga aaataattca ttacctgact tgtgggagaa attcagaaaa gtacatcata
    13621 ttgtcccaaa tactttgcca tcaaagagag tttttggaat ctgtgaagca atctctgaaa
    13681 tacacctagt tagtgaaagt atggaattta atgagataat aggacttgaa gttaatagtt
    13741 atgatgtgat tccaaattca tttgtttcaa aaactattaa ggaaggaaag tatgctatat
    13801 ttactcatac tggtagctta gataacttag acaaaactta tgaatatata tggggtactt
    13861 ggtttttaag ttcaaaagaa gaactggata taagagatga ttttgaagta tatgatgaac
    13921 gatttttagg tcctagtaat gtgaactcac aaattgatat ttatatacct ataaaataat
    13981 ctataagaaa actaaaagat aatttgatta agaaatagat gttcgtaaaa gagcatctat
    14041 tttttatttt tatcagattt agataatttt aataataaaa gaaataaaaa tatattataa
    14101 aaacaatttg attggaattt tcagaaaatt aatgctataa ttaaaagtaa atttaaaggg
    14161 aggttataaa atggctaaat taactctaga acatataaaa ggaaatacat attatattcc
    14221 actccctaca atagtaggtg tatacgtaga tggtaaagat gcaattttaa ttgacagtgg
    14281 aaataataag gatacagcta gacaagtttt aagattatta gaagaacata atttaagtcc
    14341 taaattaata ataaacactc attcaaatgc agaccatata ggaggaaatg catatttgaa
    14401 gaatcaaaca aaatgcaaga ttgctacgac taaaattgaa ggatatttta cagaaaatcc
    14461 aatattagaa tcagcatttt tatatggtgg ctatccaagt aaagcactga agaataaatt
    14521 tttattagca aaggaaagtg aagttgatta tattatacct tcaaatggca aaattgtaga
    14581 tacagaactt gaagcgattt ctcttccagg tcattatttt gaaatgattg gtgttagaac
    14641 tccagataat gttttgtttg ttgcagatag tcttacacca gagaatataa ttactaaata
    14701 tcattttttc ttcctactgg atatagattc acagtttaag acactagaaa aattgagaat
    14761 gttagaggct gatttctttg ttccaagtca ctctgtcaaa actacagata taaaaagttt
    14821 gattaatatt aataagaaga agatggaaga aataatagat aatattaaaa aggtttgtta
    14881 tgagccagtt atgattgatg aagttattga aaaaatgtgt gatttgtaca atgtcaagct
    14941 tgatgctaat caatatgtgt tggttggaag tacaatacgt tcttatataa cttatttata
    15001 tgagaataat atggttgaat atatatttga tggtggtaag atgatgataa aagctgtgta
    15061 aatagtaata attttcaaat tatatgatga aattgtttta gtatgactgt tatttgaaaa
    15121 gtaacataaa aaaatttata taaataagct aagatgattg atataaagtt actgataatt
    15181 ttaaggtaat atatacgtta ctataatatt atgttattat ttgtaaataa ggggataatt
    15241 tataagcaaa ttaaagacct taatttacag gaggaatagt gtatattatg aataaaaaat
    15301 tcaaaaaatt cagtaaaaaa ctaagtatat taattttagt tttgactatg tttcttttta
    15361 ataatttaac tgcaaatgca cagattaaag aagattcaat aataggaatt gatagatatg
    15421 aaacagcagg acttatagca gatagacaaa cttatgatac tgtaatatta gttaatggag
    15481 ataaaaattt atctgatggg ctttcatcaa gtggattagc tggagctatt aacgcaccaa
    15541 tacttttgac taaaaaaagt gaaataccaa aagctacttt aaatagacta gataataaga
    15601 gtttaaatgt tgtaaaaaaa gtatatataa ttggtggtta taatactatt gaaaattcag
    15661 tagaaaaaga tataaaaaga aaaggtattc aagtggaaag aataaatggt aacaatagaa
    15721 ttgaaactag ttataatgta gctaaaaaaa taaatgaagt aagtagtgta aaagaggtaa
    15781 tgcttacaaa tggatttgta ggagaggctg atgctatgag tatagcacct gtagttgcaa
    15841 aaaataaagg agctataata cttacagatg ggaaatcagt tccatttaag actgaaggtt
    15901 taaatgttta cgctataggt ggcaaatctg cgataaatga ggatttagta aatgaaacta
    15961 aagcaactag aatcggagga aatgatagat ttgagaccaa taaaaaggta atggaaaagt
    16021 tttataatgg agcgacagat ttttatataa caaatggata tcaattagta gatgcactaa
    16081 cattatctcc tttagccaaa gaaaaaccaa tcgttttagt tgctgatgga agtaataaag
    16141 gtattttaaa aggagctaaa agtataacaa aagttggagg tattgatgcc aatatatata
    16201 aacagtgttt agatatgatt aaatataata atatggatgt gactccaaat attttaaaac
    16261 acttaacttc agttgaagga aatgaagttt ctgaaatact tatagaatca gataatttag
    16321 gtgtggttgt tgaaaagacg aataccaata aatttgagtt tgattacgta agtgtaacta
    16381 atgaaaaaaa ttgtactttt tctgtatata aagaaaattc ttctaataat gtaaaaaatg
    16441 gaaagctagt tgtaagtgct aagaaaaaaa tagataagaa agatagtgat ttgcaagata
    16501 tgaatggcga caatatgata aatgcaaata aagataggat ggtaaatgtg ataaaaattg
    16561 gagttcccga taaaatgtat tctaatttta aagtagatgt tgaaagtggt tcagtagaac
    16621 tttataatat taaaggagga gctactgttg atgtcaatga cggaattgct aacattgttg
    16681 ataacaatgt gacaaatcct ttcaatataa atactaatag tgggatttca ggtgtaacag
    16741 ctgaaactat atcatctgag ataaaattta gagcaaacaa tggaattgta agtgtaacag
    16801 ctacaaatat aaatggcaat ataagtttat ttggaagcga taataaaggt acctttgatg
    16861 gaatatttaa attaaatctg aaaaaagaac catcaaattt acacttgaag ttagttggaa
    16921 atgggataaa taaattgcca gaaggttggt ctaaagatta tattttagga aatggacaac
    16981 caattattga agttaaaaat aatggcataa ataatatatc aattggagaa taaaaacata
    17041 t
//
LOCUS       DAMJVX010000075        16782 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMJVX010000075 DAMJVX010000000
VERSION     DAMJVX010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.078"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(160..2298)
                     /gene="vanT"
                     /locus_tag="PC407_002146"
     CDS             complement(160..2298)
                     /gene="vanT"
                     /locus_tag="PC407_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF2497591.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(2316..3122)
                     /locus_tag="PC407_002147"
     CDS             complement(2316..3122)
                     /locus_tag="PC407_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2497592.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(3119..4219)
                     /gene="vanG"
                     /locus_tag="PC407_002148"
     CDS             complement(3119..4219)
                     /gene="vanG"
                     /locus_tag="PC407_002148"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2497593.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(4476..5558)
                     /locus_tag="PC407_002149"
     CDS             complement(4476..5558)
                     /locus_tag="PC407_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2497594.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            complement(5608..6309)
                     /gene="vanR"
                     /locus_tag="PC407_002150"
     CDS             complement(5608..6309)
                     /gene="vanR"
                     /locus_tag="PC407_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HDF2497595.1"
                     /translation="MNENILIVDDKKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(6743..9274)
                     /locus_tag="PC407_002151"
     CDS             complement(6743..9274)
                     /locus_tag="PC407_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2497596.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVKEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(9382..9999)
                     /locus_tag="PC407_002152"
     CDS             complement(9382..9999)
                     /locus_tag="PC407_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF2497597.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIITPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(10361..11245)
                     /locus_tag="PC407_002153"
     CDS             complement(10361..11245)
                     /locus_tag="PC407_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2497598.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLVLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAVKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(11436..12686)
                     /locus_tag="PC407_002154"
     CDS             complement(11436..12686)
                     /locus_tag="PC407_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2497599.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFIYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDCKAI"
     gene            complement(12908..13672)
                     /locus_tag="PC407_002155"
     CDS             complement(12908..13672)
                     /locus_tag="PC407_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2497600.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(13666..14391)
                     /locus_tag="PC407_002156"
     CDS             complement(13666..14391)
                     /locus_tag="PC407_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2497601.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(14393..14761)
                     /locus_tag="PC407_002157"
     CDS             complement(14393..14761)
                     /locus_tag="PC407_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2497602.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(14937..16481)
                     /locus_tag="PC407_002158"
     CDS             complement(14937..16481)
                     /locus_tag="PC407_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2497603.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQFISKYAIQIILIMAIICMMLIFYIIYDKLNFKIKLQKIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIDLNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         5734 a         2953 c         1679 g         6416 t
ORIGIN      
        1 aaggtgatgt ttcaaaataa aaatctgaaa catcaccttt ttataactta aattgtcatg
       61 taagttataa aaaatatata ttagacataa tattactaaa aatgtctagt cagagtaaaa
      121 taaattacac agtacaacaa atattactat ttaattgagt tacttcttta taacatttaa
      181 ccttcttcct actctgctca ataattcatt cgatatacta catgagttat ttgcaacttc
      241 agaagcagat aattcagata aattatctct tcctattatt attgccgtat taccaacttc
      301 tacattaggt atatctgtaa tatcaacagc aagttggtcc atgcaaattc gacccactat
      361 tgctgctcta caaccattta ttattacgta actttttcca caggataaat ttctaggtat
      421 accatcggca tagccaatag ataatatagc aattacgcta tctctgcttg caataaagac
      481 tcttccatat ccaaaactat catctttttg tatttttcta ataagaatta cttgtgattt
      541 taaggataaa acaggtcgta aatcaagttg caatttagtc gtatcatttg gagaacttaa
      601 tatcccgtat aatgcaattc caattcttac ataattacac ttaagattgg gataattaaa
      661 aaaaccataa ctactttgaa tatgtacttt aggaattttt ataccttttt ttaacagaaa
      721 atcaattaac ttataaaaac attcaatttg tttatttgta aagtctacat cttcatcttt
      781 cagactatct gaaacacaca agtgtgtaaa tatgccacat atatctaagt attttaattt
      841 aaatgccttt aaaattgacc tcacatcatt aacatcaaaa cccagtctgt gcatacctgt
      901 atcaatttta atgtgtgatt ttatgttgta tttctgtttg tttaaagaga tagcatgatt
      961 aaggtcaata acactctgtg taagattgta tttatgaagt tcctttgccc ttattgggtt
     1021 agtatatcca agaatcaata tttctcctag tattccatat cgacgtaaat caatcccctc
     1081 atctattgtt gctactgcaa atgcttttac acctatttgg tttgcacaag tcgctacttc
     1141 aaaagcacta tgaccatagg cattagcttt tacaactgcc atcaattcac aatcttctgg
     1201 catagctttt tgtatcatct ttacattatg ttccaaatta tttaaatcaa tttcaatcca
     1261 agctctattg gtatttacct tatgttctcc tttcaagtct ttttttagag gtttaagaat
     1321 caatataata attatagaag ctataaaaga tgctatactt accactatat aatgaataag
     1381 gctattgtca accaagacag tttgtattcc gaatatttta gaaaacatac gaataacaac
     1441 aatcatcata ggatgtataa tataaataat taatgatgat gttcgcaata acctagctcg
     1501 agaacctttt aaaaatgtta aggctgtaaa taagaaatac atacatggca ataacataat
     1561 atacatgcta tcatgacgct gtacccctag tttatgtaaa aacattccct cacataacat
     1621 aagtaagaat gatataagaa atccaatcaa gctattttta gttgacatat aattagattt
     1681 attagatatt attccaccaa gtatgaaaaa tacaggtgca aaaaatatac catttcgtgt
     1741 ataatctgat acttcaaaaa tatacccata taaattcttt aatagtggta ttttttctaa
     1801 aaaaccataa taactatcac caaacatacc aattacataa ataacaatag tgattactaa
     1861 tgagtacttt aaacctagtt tttttacaag aaagtatgca atacttgcac caataattga
     1921 tgcaggtaga taccataaat gatataaagt cccatcaaat acaatgtcct taataatatt
     1981 gggcactaaa ttattcatgg aaaaatatcc agtgtaaata tttaagggaa tataaattac
     2041 aatagctatc ccgtaaataa ataatacatt ttttagaaaa gatttcagtt tattacaatt
     2101 ataattatat gttgaaatta gaaaaaagcc tgatagcata aaaaagaatg gcacagcaat
     2161 acgagcaatt atactagtta acacaaagtc agccatttta ctatatgata ataatggaga
     2221 tgtatgaatt gcaattataa gtaatgcagc tataatccta aaataatcaa gccctgcata
     2281 agatttattc ctaatcattc ttgttcctcc atttcttagt cctttctatt tctccaaaat
     2341 gtcacaataa aaccatcctt attgttaccc gatatttgat atacacattg ttcaggtata
     2401 gagattctca cttcttcttt agtatgaata ggcacataaa agatttctat aattttttct
     2461 ttatcttcta tttttaaaca atttgtatac gaaaatctct taatatattc tatatattct
     2521 tcaagggcga gattcatttt attgataatc tcagaatgtg gatatccgac ataacgaaaa
     2581 tgccatggtt cttgagatat tcctgttatt tcttcttttt tacactgata acgctctata
     2641 aaaccataat gtagtgctgc cttacggaac tcattacaaa taccattata aggaaattca
     2701 gggcagataa aatctataac ctctttcttc aaccctaaat caatagcaag ccctgtctga
     2761 tgttcactgt gatttggaag tgctacaaac tttttagtaa agtcttttcc attgtcacgc
     2821 agagagcttg aatatatatc ttgttgttcc tctgcagtac gatatccact tacaggtact
     2881 atttcattta ttccacatat actttttaaa atattttgta aaatatttgt agcttcacgt
     2941 ttcataagaa tacttggaaa ccttgtatct acaggaatta aatctatatc ttcagatgtt
     3001 ttaactggta aaaaagcatt tactagaatt agattcccat cataaacttt atctttagtc
     3061 aataagatag tatttgtcat tttatcttct ttatttttat ctaataaaat attattcatc
     3121 attttatgta cacctcaatt atcttatcca atatttcttc aaaagacagt ccaatacctt
     3181 tcatcatatt cggataacga ctatgagaag taaagccagg aattgtattg acttcattaa
     3241 aaataatttc accagatgga gttaaaaaca tatctactct agcaaatcca gaacatccaa
     3301 gagttctata aataattttt gctgtctctt gtattctttt ctcacaatcc aaatcaattc
     3361 tagctggcat atgaattttt gaacttttca atgtatattt ttctgtataa tcaaaaaatc
     3421 cttttgacaa ttctatttca tctactcttc caacaagtaa gtcatcgttt cctaaaactg
     3481 cacaccctac ctcaaaacca tcaatatttt cctcaattat tacttcatta tcatgttcaa
     3541 aagccaagtc tactgctact tgtaactctt cttttttata aattttacta ataccaaatg
     3601 atgaacctgc acgcacaggt tttataaata atggataagt taagtctttt gacagttcag
     3661 ccacattaaa tttatctgag tattttagta atattgattt tggtacttta ataccttctg
     3721 aatgaacaag tttatgtgat ttgtctttgt ccatacataa tgctgaagaa agagtaccac
     3781 atccaataac ctgtatacct gccaattcaa ataacccctg cactgttcca tcttctccat
     3841 tttttccatg caatacagga aatacaaaat caagtttagt gtatttaata tcattttcac
     3901 atgattcaat agcactattt ggaatatgat tctgagaaac agcatcagga attaaaaatt
     3961 caataatacc atttttgaca cggttctgcg aaacaataac tggaattata tcagtatccc
     4021 tttcaaacca agtgttattc tggatattat tataatctcc aatataatga taccaattac
     4081 cctcccttgt aattccaatt ggtataatat catatttttc cgtattcaaa ttctcaagta
     4141 ctgcaaaagc tgattgtaat gatacatcat attctgttga gtttccacca aatataactg
     4201 caattttttt attattcatc tttgattctc ctttgttgat ttatttattt ttatacaatc
     4261 tacagaaaca tgttaaaata ctttttctat ttcaaatcca atcaaccttc acttacaatt
     4321 gcatctgttc cacataatat attttcacat ttttctattg atataattac tgtatcaaaa
     4381 taaaagcact cttgttttga tacagtaatt atatcaataa caagagtgct ctttatttat
     4441 cctaaagaat ttcctatttt tttatactta aattcttaca aatttcctac aatcggaata
     4501 ataaccttaa aggtaattat attgttctcg ctatgagctg ttattgtccc gttatgaagc
     4561 tccactattt ctttagctat agcaagtccc aacccagctc taccactgtt tgaattacga
     4621 gatgtatcca acctgtaaaa ttgttcaaaa atacgctcaa gtttttcctc tggaatcgta
     4681 ttgctatgat ttataaattt aagctttata taatcatcct cctgtacagc tgttatttca
     4741 atagtagtat cttcaaaact ataataaaca gcatttcgta ataagttatc aaatacacgt
     4801 tgcattttat taacatcaca ccttatcatc atatttggtg taacattcag tgtatatttt
     4861 aaattcttac tcatcaaagt aggtttaaac tcatatgtta gttgttcaag catacgagtt
     4921 aagcttactg tactgtattc aagtgatata tttgataaat taaaccttgt aatttcaaag
     4981 aattcattaa ttaaatcttc aagacgctct gccttatcca acgaaataga taaatatttt
     5041 tttcgcagtt cttcagaaat ctgactttca tctcgaagta gtgtcagata ccctataact
     5101 gatgtaagtg gtgtttttaa atcatgtgca agataaacaa taagttcatt cttacgttgt
     5161 tctgccattt gtgtctcaaa tttacgttgt tcaagtatat gtttaattga gtttattttc
     5221 ttttctattg ccaataactc tggagataaa gccacttctc caacatcttc tttgataaga
     5281 gagtcaatac cttgatttat ttcactaaaa tattttgtaa accaatttaa ataaacacgg
     5341 aatattataa aaaacacaat caccatagct agtatcatga agagctccat gctattacgg
     5401 aaaatctgct gatataaatt caatgcagct ttatattcca tacgaaaagc attttcaaag
     5461 aatgatacta tccagataga aaacttacca tacaatataa aagaatataa aatccatatg
     5521 aaaataatgg ctataaatgc cattaaagct gttctcataa aaattttatt tctcacttga
     5581 gtaaagtcag gattgacttt aatattttta cttttcaatt gtataaccta ctccccatac
     5641 agtttttata aatttcgggt ttttagaagg ttcatgaatt ttttcacgta atcgccctat
     5701 atgagccata actgtgttat tattatcaag atatttttct ccccatacag cttcaaacaa
     5761 ttcttcagaa ggaaccactt ttccttgatg ttcacataaa taccaaagaa tagaaaattc
     5821 aatcggtgtt aatgctactt ctttcccaaa taaactacat ttatgtgttt ctttattgat
     5881 aatcaatcct ttaatatcat actcattaac aattatcgac tgttgttcat aagaattatt
     5941 atatcgcata tatcttctta attgtgtctt tactcttgca acaacttcca aagggttaaa
     6001 tggctttgtt atataatcat cagcacctat ggttaaaccc attatcttgt ctatatcttc
     6061 aattttagct gttaacataa tgataggata atagtatttt tctcgaattt tctggcaaat
     6121 atgaaatcca tcaatatcag gaagcataat atctaaaata gccatatcca aatctttaga
     6181 ttcaatacat tctagagctt ccttacctgt ataaaacttg tatactgtaa aaccttcatt
     6241 ttttaaatat acttcaatta aatctgctat ttctttttta tcatccacaa tcaaaatatt
     6301 ttcgttcata gattattttt cactctcctt attatcgagg tcatcattta taatattggt
     6361 aaatttacat atcaaataat tacaagcata tttgtaacta atttttataa gaaaagaact
     6421 actacatcag aatgtgttga tgctactatt ggaacacaaa aactgagatt aattgttatt
     6481 ataaaaactc ccctaacacg tccttattat cgcataacag gtattaactt tcaataaaaa
     6541 tatctatcag tcaaacacaa ttcaatttat tataccagag acaatccata taaaaaacaa
     6601 ttaaacctat gtatgtaacc acagacatta ctaaaatttc catgttattt tatataaaaa
     6661 ttcaaaaaag gagttgaatt taaaatttga ttagaaaaaa atcaattttg agacaactcc
     6721 tttttatttt aatgtattta aattatagta tatcagcctt cataatatcc tcaaaatcac
     6781 ctttttcaga tattaatctt atcaatttaa cagtacagct taaatcattt ataagtatag
     6841 ctccaactaa tgaaccatct ttcataaata attttttata tattctttga ccttcatctt
     6901 ttgatgaaat ttctttatca aaattttgta tattaccaat actaaatact tttacattca
     6961 ttgcatctaa tgaaactgga tatacttcac tttcaaatgt caaattatct ccacttgcat
     7021 ttgctcctgc tatttttccc atttctactg aagatggcca tattgctaaa ctaatatttc
     7081 ccacttgtgc aacatctcca caagcatata tatctttaac atttgtctgt aatgtcttat
     7141 ctacaacaat acctctatca atttttacag atgtatcctg aaccatttga gtgtttgctc
     7201 ttacaccaat tgaaaatact accatatcac agtcaacact atctccatct ttaaatacaa
     7261 ctttttgagc ttttccatca ccttcaatac catcaactgc tacacccaat cttacatcta
     7321 ttcctgtatc tcttacacat tgttctaata ttttactacc ctctttatca agttgttttg
     7381 atagtatgct atccatggct tcaactacta ctacttctaa tccttttagt ctaaattccc
     7441 atgcagcttc aagtcccaat aaaccgccac caatcactac aactttctta gatttttcta
     7501 gtgcttcttt tacactatat aaatctttta tatttctaag agtaaacaca ttttctaaat
     7561 catggccctt aatcggtggt ataaagtttc tacttcctgt agctattaca agcttatcaa
     7621 atttaatagc tccatcattc actataatag ttttattcac ttcatctaat ttatctacat
     7681 tcgttcctaa tattactact atattatttt tatcatacca atctttatct tccatgtaga
     7741 aatctgaacc aagttcttca tttataccat cagaaagtgc aggtctatag taagtcaact
     7801 catcttcatt agaaatcata gttatcttac aagttttatt tctttttctt atagcatctg
     7861 ctgcataaaa accagctgct ccatttccta ctattacgaa atattcatca gtatcttttc
     7921 taaaagtaac ttcatcttct tcaacttcta taaattggtc ttccttagct ccacatacag
     7981 ggcatacact aggagcacta tcaccttcaa aaacttcatt acaaattaca catttaaatt
     8041 tcttagtttt ctttttactt ccaaaagtag aatttaactt atctaggaat ttacctgtat
     8101 aagaatgtaa tttatcaatc tcttcatttg atggtttaaa gttaacagca aaaggtttta
     8161 ctgctgtcat tctaagctga cttattctct ccatagcatt ttctatacct tctccactcc
     8221 atccatatga accaaatact gaagcaactt ttccaccatg aagaactggg tttaacgata
     8281 ttaatatatc ccatataggc tttaacgcat ctcctaatat tgttggtgtt ccaaataata
     8341 caccttgtga tactgctatt tttgccaaaa tatcttcttg cttatgctct attacatcaa
     8401 ataaatttac tttatagttg ctattttttt caatatatgc ttttatagct tctgccataa
     8461 tctttgtata tccatgtgca gaaacataac aaattgtaac ttctttttct aactttattt
     8521 gttcctttac actccagtta taataaaggt ctataatttt tctaggattt tcagttaata
     8581 ctggaccatg acctggacat acagtatcta tatctaaatc ttttatcttt tctatagcag
     8641 ttaccataga tggtttaaat ggacccatta tacaatcata atagtatctt aaagcatcca
     8701 agtaatcctt ttcttcattt tcatcaagtg tatttactat cttatcatta ctatagtgac
     8761 taccaaatga atcacatgtt acaagagttt tatcctcttt tatatatgtg tatatagtat
     8821 ctggccaatg aagcataggt acagagaaaa attctaaagt tttgtcccca attgataatg
     8881 tatctccatc tgtaactgca acatattcaa agtctttgtt tacgatttcc ttaagatatt
     8941 taattgctgt ttctgaagct actacttttg catttttagc taaatccaaa agcttttcaa
     9001 cgcttcctgc atggtctggt tcagtatgac ttacaacaat atagtctatt ttttcaaaat
     9061 ctatatttaa atcatttaat ctttcaatat aattgtcaaa atgcttatct ttaactgtct
     9121 caaacaaaac tgttttttct gttcctttta atacatatga attataagta gttccataag
     9181 gtgtatacat aattatgtca aatactctta aatcagggtc aagtgaacct acccaatata
     9241 aattttcttt tatttttagt gatttcaatg acataccgta ctcctcctaa aatagttatt
     9301 tagtctcttt ataccctgct tgttatattt taaacaaaat ttattcaaaa aagaaattat
     9361 tttaaagata gtttaaatct tctaatcttc tttttctacc ttcaatacag ttcccttttt
     9421 agaatctact tttatgtcat attcattttt atctttatct attcttattt tataaaaagt
     9481 aacatctaaa tctttgtcta aactccattc tttaaccatt ccattttgtt cttttaatgc
     9541 aatttccata gcttgttgag gtgtaattat gtcatttaaa tctaattttt cttttctagc
     9601 aactccattt aaatcttctg aatctaactt ttctgtttta tcttctaata catcttttgt
     9661 attagcatca attttcatct tatattcatt attatcatct acaccttcca cagtataaac
     9721 aaatttattt aaatctctct caagacttaa atctactata tcagcatcct tgtatttatc
     9781 tttaaatatg tttattgcat ctgtgtatgg cactttaaaa tcttgttcca ttaaattttc
     9841 attatttttt tcatctttta aagcattatt tgaagcattt gcctcctttt ttgtattatc
     9901 ttgtgcttgt cctgaatcct tagttgggct cccactacat gcagttaata aacctgctat
     9961 aagtatactt accactaata aatacgtttt tttacacata aaaatcctcc ttaaatatat
    10021 taatttattc tatattatgc cctctaaacc ctttatttac ccttttatat taaaaattcc
    10081 tcctatttta tcaattttaa aactttatat ttactaaaat taaagttaca taaaaattat
    10141 actgtctcaa aatagaaaca agcatctttt tcattgttga aataataaaa atatatttta
    10201 tttcgtacaa aaagagtgcc tcatgaacta aaaagttcat tttgagacac tcccattctt
    10261 ttatattttt acttacaaaa taacagtact tattaatttt ttacaaacct tataagtata
    10321 tctatattat attcttaaat ttaaacacta tattaaaaaa ttaaattttg tttaattctt
    10381 ttactgttaa atccatttta ggatatggaa tcgtaattcc ttcttcgtca aacctaactt
    10441 taacctcttc tagtaaatca agataaattg tccaataatc ttctgtcttg caccaaactc
    10501 tagttgcaaa tttaacagca ttatctccat gttctactac tcctataaat ggttctggct
    10561 catttattat taatttatga ttattaacaa tatctttaag tactcttcta acatgtagta
    10621 tatcatcttc ataccctaca ctaaaagtca aatcaactcg tctagtattc tttgctgaat
    10681 agtttattag actatcattt gatacagaac cattaggtat aagtatctgt ttattatctg
    10741 gagtaaccaa ttgtgtataa aaaagtccta tttgttcaac cgttcctcca tggcctgctg
    10801 cttctacgta atctcctact ttaaaaggtc taatcaatag tataataaat cctccagcaa
    10861 aatttgatag actaccttgt aaagccaaac ctattgctac ccctgctgat gctatcacag
    10921 cagcaaaact ggacaaactc attccccaat attccagaac aatgattata acggcaactt
    10981 taagtacact caaaagaaga gacttcaaaa accttcttaa agttaaatca atatctcttt
    11041 tactaagtac taacataaca tggtttacaa atttttttat tattttaaat ccaatagaaa
    11101 gtataagtat acctattata agttttactc cgcttgtagt agcccattct agcagacttt
    11161 ccattatacc tcccatatcc atcttattaa tgtctaaatt atttaaatct aacttgctta
    11221 tatcatctat tttcgtcgta tccataaaaa actccttacc ttatatttta gaaccaatat
    11281 tatagtttaa agttcttatt ttctatttaa tataatacaa taccatttgt acatagtaaa
    11341 caagatttat attccaatta tacatcttag gaaaatgcaa actatttgtt atatatatgc
    11401 aaaatataat actaaattaa actattaata aaacactata tagccttaca atcatcattt
    11461 tttaccccta tatgagatga tactatagtt ataatactta gtactataaa tactacaaaa
    11521 ccttttttat atccatcaac tatattatat gaatttactt gtaaagagat agctataatc
    11581 aaattaataa ttccacttcc tataaattca aaagtattaa taacactagt tgccagtcca
    11641 gaataaattt ctttattttt atacttggca tcattaaaaa cctgtagatg tgacataact
    11701 atacatccca taataaagaa tgatattggt aaaactataa ttggtggttt tacctggtat
    11761 acaactacaa ttacaatcca tataaacaaa tctatcattg ctccaaattt tattatatta
    11821 aatttgctag accttatttt agcaaataca aaatccataa ttattgaacc aaatataaat
    11881 ccatatgtaa aaaatgatac tataaacgca gaaaaactct tactcacacc ataaacgtcc
    11941 attatgtatc ttactcccca caagcttata aatgcacttg taagaccaac aaaagagaac
    12001 attatcattg aattatacca tgttgatttg ttctttataa cagacttaat accatcaaca
    12061 atattaacct tctctgactt ttcatagggt tcagtatcta cattgaatcc atactctttt
    12121 ggtgtatctc taacaataat atatataaaa catccaatta caacaccaat tataccgatt
    12181 aataaaaatg aatttctcca accaacaagt tcagacaaaa acacaagtgg aaaagtagca
    12241 agaactccac ctaaatttcc tacaaatgaa aatttagctg ttgcagaagc aaattctgac
    12301 ttattaaacc atctcccttg aatttttaaa atacaaagta atattactga tgttcctgcc
    12361 cctacaatta cacgagaaat atatgctaat tctatacttt gaatcaaacc aaataaaatt
    12421 gaccctaaac ttgccattat tattccaaga ctgctaattt ttcttgctcc atatttatca
    12481 atcaaaattc ctgcaggtat ttgcataaat gcatatgcat ataggcaaaa ggatgctata
    12541 tttgaaattt gtatagaatt aaaacctaac tcatttgcta aattgtctga aacaacagca
    12601 gttgacatcc ttaaaaataa tactaaaaca aatgaaaatg taattatacc ccaaacactc
    12661 cacttagcct ttgtataact tttcatatct tctccctcaa atccatatat ttttaaattt
    12721 tcttgatata acttatataa agtgtatagt tattcttaca aatttttaca cgcaatatca
    12781 ttatacaaca tatgtatata tttaactaga aataaaatac tattcatatt atatacatga
    12841 attaactaca taaataataa gagccatctt ttaacattac tcaaaagatg gctccatata
    12901 aaaacattta ttctaaattt aattttttat ctaatataaa ttttgtagcg aaaaataacg
    12961 ctattgcaac tacaagacta ccaagtattg caagcataaa actaggactc tgataaagat
    13021 gatataccat atttgtatct tcatttggta agatgcttcc aactgcatct ccaacaattg
    13081 atattactat atttatagct ataaaagaag caaatgccac tatgtttcta tgtttattga
    13141 attttggtaa ttgtcccatt gagattgatg tatataataa caaaataaat gatgaatatg
    13201 aaataaacat tactactact aataataaaa tatctaaaaa gttttcagaa atccatttaa
    13261 aactagtgtt aaacaaatct aaaatttcag gtagtaaaat tccagaagta ccaaaaagca
    13321 taagaactcc aaaagtgaat actgcaacta taaaactaag tacagcgtat ataagtgctg
    13381 ttatacattt agataatata agtgaactag tgctaacagg aagagtaaac attaaataac
    13441 cttcatcccc caaaagattt tttctaaacc tttgaattgt aactacaata gttaaaactc
    13501 ctaatgccat aaaaagactt gttaatacta gtattcctat tccctgtacc tgaagtatat
    13561 ttgtattcat aaatattcca ttaaatacag ctactatcaa tatggctata tataacggta
    13621 taaatattct tccacttgct tttaactcat attttaataa tttacctaac atttaaattc
    13681 ctccctaaat aatgcatcta tagattttcc tttttcttct cttattgctt caacgtctcc
    13741 ttgtaacact atttctcctt ttgatatgaa tattatctca tcacatatgt tttctatttc
    13801 acttattaag tgggtagcaa tcagtaaagt actatcttca gaataatttt ttaatatagt
    13861 cttaagtata tatgaccttg cagctgggtc aacaccacct ataggttcat ccaatatata
    13921 tatgctcgca tttcttgaca ttactaaaat aagttgaact ttttctttag ttcctttaga
    13981 cattgtcttt agcttttcat ttacatcaat atctaagctt ttaatcatct catttgctct
    14041 tctaacatca aaatcactat aaaaatcata gaaaaactta agtaaatctg ataccttcat
    14101 ccaatcattt aaataagttc tttctggtaa atatgaaact atttttttag tatctatgga
    14161 tggtttcatt ccatttatca taatttcacc attatcaggc tgtaataatc cattcattag
    14221 cttaatcatt gtacttttac cacttccatt aggccctaac agcccgacta tttttccctt
    14281 tggtatattt aaatcaatat tttttaaaac attcttagtt ccataactct tgtttacacc
    14341 tttaaattca actattccat cttgagaaac attgcttata tttttatcca ttttataatt
    14401 ctcctttcag attttctgta ataagtttta gcatttcatc tctatcatat ccaagctttt
    14461 ctaaggttac ttttaatgta tttatctcca cattagctat ttcctctttc atagctttta
    14521 ttttttcctt atcatctgtt acaaatcttc ctttagttct ctgactataa actaaacctt
    14581 gtctttcaag ttctgttaga gccttttgca ttgtatttgg attaacttcc gcatcttctg
    14641 ctagtgctct cactgtttct aatttagatc ctattttaat ttcccctgaa attattttta
    14701 actttaaatg ttctactaat tgtatatata taggtttgtt attatcaagt tcccattcca
    14761 tgttcatgcc tccttattat attattgtat taatttatta atacaataat ataataatac
    14821 aaagtatatg tcaattactt tttagtaaaa gtataaaaaa tttcacaaaa aaaatgaaca
    14881 ctacattaga gtccattttt aacacaattt attaaattct ttatattaca gattaattac
    14941 cctcttacca agttggatac aattaaatgt tcaaattctt caattggcat tggctttcca
    15001 aacaaatatc cttgtgccat atcacacttt acttctttta aaagtttaaa ctgttcttct
    15061 gtctcaactc cttctgatat gactttcata cccaactctt ttgccatttc tattacttta
    15121 gtaacaatta ttttgcttct tttactatta taagtttcca taataaattt ttggtcaagt
    15181 tttaagatat ccataggaat ttcttttaac atatttaatg atgagtaccc tgaaccaaaa
    15241 tcatccatag atattaaaaa tcctattttt tttaaattat tcattatatc aattaaaata
    15301 tcaaaattat caaatatgat actctccgtt aattcaagtt ctatatactt tgcaggtata
    15361 ttgtatttat caattatatt tttgtatgtt tctataaaat tattgcaata taaatgtatt
    15421 cttgaaacat taactgaaac agtaactgga tttattcctg catcaatcca ttttctaagg
    15481 tatatacata cttcttcaag cacatataaa tctaaattta taacaaaacc gtttctttca
    15541 aaaaatggga taaattcatt tggaggtatt agccctttat caggacgttt ccacctaaca
    15601 agtgcttctg aaccttgtat ctctcctgta ttaagatcta tcttaggttg taagtatata
    15661 ataaactctt tattttctaa acttgaaaac attgtatttt ccatctcttt ttcttttatc
    15721 tcttggtcat gaatcttatt atcataaaat gcatatgaac ttttatgtcc tccctttgcc
    15781 attttatgag gtatattagc cctatctatg attgagttga tatctctatc ttctggaagt
    15841 ataaaataga ttccacaact taaaactaac ttatactttt cttcttgatt attattaaac
    15901 aagtctaatt cctcaaatat agtttttaac ctctttgtta tatcttcttc ttctatatat
    15961 ttcataatta taataaaatt atccgcagat attcttgcaa acatttcctc ttcaaatata
    16021 ttactcttaa taatttttgt taaattacgc aaaatttcat tcccaacttc atatccaaat
    16081 aaatcattta tatatttaaa tttatcaata tctatatata ataaagcata ctttacttga
    16141 gtatttttac atagtatttt gttagcatca attacaaatt tatctatagt atttgctcct
    16201 gtaagattat ctgtatatgc tatcttttgc aatttaattt taaaatttaa tttgtcatat
    16261 attatataga atataagcat catacatatt atagccataa taagtattat ttgtattgca
    16321 tatttgctaa taaactgatt attagacaca atagactcaa agttatttat ggagttgttt
    16381 ttatagagtt tgcataaaga atagactggt gttgtgtatg taatttgtaa aattatacaa
    16441 ataaataaaa aaaacatcaa ttttttttgt aaattcctca taaaaatttc ctcccattaa
    16501 aacacctcta ttttattagc aaataagtac atctcaaaag tttccatttc ttgtaaaatg
    16561 gatagtttat atctacttta attttgtaaa attttctgct actataatta tagaatgtag
    16621 catattcatt gtcttaacta ttgatttata tatttcatat aggtataata gaaaataaat
    16681 tggattattt tggcatataa ttatttatta aaaggtgatt atttttgttt gaattgtctg
    16741 aaaaatttta tttttatatt ataattttaa tattttcttt tc
//
LOCUS       DAMJVX010000076        16668 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMJVX010000076 DAMJVX010000000
VERSION     DAMJVX010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16668
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.079"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(72..275)
                     /locus_tag="PC407_002159"
                     /pseudo
     CDS             complement(72..275)
                     /locus_tag="PC407_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
     gene            complement(275..604)
                     /locus_tag="PC407_002160"
     CDS             complement(275..604)
                     /locus_tag="PC407_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HDF2497604.1"
                     /translation="MPQIKIRGINENDICKISKKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            934..1860
                     /gene="fba"
                     /locus_tag="PC407_002161"
     CDS             934..1860
                     /gene="fba"
                     /locus_tag="PC407_002161"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HDF2497605.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(1995..3626)
                     /locus_tag="PC407_002162"
     CDS             complement(1995..3626)
                     /locus_tag="PC407_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2497606.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(4109..5146)
                     /locus_tag="PC407_002163"
     CDS             complement(4109..5146)
                     /locus_tag="PC407_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2497607.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(5165..5950)
                     /locus_tag="PC407_002164"
     CDS             complement(5165..5950)
                     /locus_tag="PC407_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2497608.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(5983..7116)
                     /locus_tag="PC407_002165"
     CDS             complement(5983..7116)
                     /locus_tag="PC407_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2497609.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(7164..8291)
                     /gene="hadC"
                     /locus_tag="PC407_002166"
     CDS             complement(7164..8291)
                     /gene="hadC"
                     /locus_tag="PC407_002166"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HDF2497610.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKNETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(8291..9517)
                     /gene="hadB"
                     /locus_tag="PC407_002167"
     CDS             complement(8291..9517)
                     /gene="hadB"
                     /locus_tag="PC407_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HDF2497611.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(9532..10332)
                     /gene="hadI"
                     /locus_tag="PC407_002168"
     CDS             complement(9532..10332)
                     /gene="hadI"
                     /locus_tag="PC407_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HDF2497612.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(10371..11570)
                     /gene="hadA"
                     /locus_tag="PC407_002169"
     CDS             complement(10371..11570)
                     /gene="hadA"
                     /locus_tag="PC407_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HDF2497613.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            12247..13245
                     /locus_tag="PC407_002170"
     CDS             12247..13245
                     /locus_tag="PC407_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HDF2497614.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            13607..14134
                     /locus_tag="PC407_002171"
     CDS             13607..14134
                     /locus_tag="PC407_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HDF2497615.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            14161..14757
                     /locus_tag="PC407_002172"
     CDS             14161..14757
                     /locus_tag="PC407_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HDF2497616.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKDVIDTVSISLNAPNKEAYNRVTQPKFGEKSFDYMLGFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(14822..16405)
                     /gene="asnB"
                     /locus_tag="PC407_002173"
     CDS             complement(14822..16405)
                     /gene="asnB"
                     /locus_tag="PC407_002173"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2497617.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
BASE COUNT         5153 a         2971 c         2107 g         6437 t
ORIGIN      
        1 gtttccctat atgctgacta ttttagctac taactcatga ctaaagtcac gagtgtacgt
       61 agctattttt taatcaaata tatatattct ctatatataa cttcttctcc attaaataat
      121 atctcatcat tttcagtgtc tacttgagtt cctggatttg aaacttcact tccatttact
      181 actacagacc cttgtttaca atacttcttt atttcacttc tacttccata acctaaattt
      241 gaaagaattt tatctatacg ttgcttttta gccattaaaa atgctctcca ttctcataat
      301 atttttcttt ttcaaatact gtaaatgcta aatccatact ttctacatct aaattatttc
      361 ttatactatc agttattatt cttgctacta tatcttgtac ttcttgtcct ctatcaaacc
      421 aagctacttc aacaaaagga tatacatctg ctattttacc atctcttatt gcaactgact
      481 ttatacattc tatttcaaag tagtctcttg gacattttac agcttcaact aaatcattta
      541 tcattttttt acttatttta catatatcat tttcattaat tcctcttatt tttatctgtg
      601 gcatagttta tctcctttat tttaaatttt tagtgaatta tcatccactt aattcccttc
      661 gataacgttt gaagtggtaa cttcttggtc aagacaacta ttgttatcag tatctccaaa
      721 ctaataaggt agttcctacc cttatagtta gcattatata gctaattttc ctaaattatt
      781 tatattcttt attattttat ttaatcctat tgtcttctat ttaacattat acttgtaatt
      841 tatagttatt acaatttact atatactttt tttgatttaa cgttataata taagtgatgg
      901 gtaaatttta aaagttaata ggaggagttt aaaatggcat taattactac aaaagaaatg
      961 tttaaaaaag cttacgaagg tggatttgct ataggtgcat ttaacataag tgacttagaa
     1021 caattacaag gtgttttaaa agctgctaaa gctaaaaatt cttatgttat gatacaagct
     1081 tctatgtcag ctgttaaata tgctggacct catacattag ttgaaatggt taaggctgct
     1141 tctgatgaaa taggtgtaga tgtagctctt cacttagacc atggtccaaa tatggatgct
     1201 attaaaactt gtattgatgc tggtttctct tcagttatga tagatggttc tcattttgac
     1261 tttgaagaaa atgttagaat aactaaagaa gctgttgaat atgcacactc taaaggtgtt
     1321 gttgttgaag cagaacttgg tgttctagct ggaacagaag atgatgttac ttctgatgtt
     1381 cacaagtata ctcaaccagc tgaggcagtt gagttcgttg aaagaacagg tgttgattca
     1441 ttagcaatag ctattggaac ttctcatggt gctttcaaat tcaaaggaga agctaaatta
     1501 agatttgata tactagaaga aatccaaagc aaattaccag gtttcccaat agttcttcat
     1561 ggtgcatctg ctgttgacca aaacgctgtt gctacatgta atgaatttgg tggaaatata
     1621 gctggtgcta aaggtgtacc tgtagatatg ttaagaaaag catcttctat ggctgtttgt
     1681 aaaataaaca tggatactga cttaagacta gctatgactg ctgctatcag aaaattctta
     1741 gctgaaaatc caaaagaatt tgacccaaga aaatacattg gtgctggtag agatgctata
     1801 caagctgtag ttgaaagcaa gatagatgat gttttaggtt ctgctaactc tataaactaa
     1861 tataactttt aaatatttat aaaagaaaag agatgactta aagtaattaa atttattttt
     1921 aagccatctc ttttaagttg tatcaattat gtatcttata atattttaat tatattgtct
     1981 ttttaaactg acagctaaac gtgaatatgt ccactcttta tctttctatg gatggtagaa
     2041 ggatatatac ctataacttc agcagccttt gttattgaac catacttatt tatagcctta
     2101 cttatcatta tttggtccac tattttatat gcatcattaa gattcataat tccatttacc
     2161 actattggtg ataaatcatc tgtttcatta tcaatcatat ctaaatttat cagcatgtta
     2221 aattcatctt ctccaattac atttttagct gataatacta caaatctttc aatgatgttt
     2281 tttaattctc ttatgtttcc tggccaagaa tattcttcta ataactcaat aactttaggt
     2341 gaaacttcta cttctctatt gtacttttca ttgtaaagtt ttaagaaata atttactaat
     2401 ggtactatat cctcttttct ttctctaaga ggaggtattt ttactggaat aacacttaat
     2461 ctatagtaca aatcttgtct aaatttaagg ttatttctaa ggtcttcatg agaaatatta
     2521 gtagcactta tatatctaac atctattttt ataggcttac taccacctat tctagtaata
     2581 gtattttctt gtataaccct aagtaatttt ttttgcatct gcattggtaa ctcacctatc
     2641 tcatccaaga aaatagttcc accattcgcc tcttcaaaaa gtcccttttt accttttttc
     2701 ttagctccag taaaagaacc ttcttcatat ccaaaaaact ctgactctaa taattcattt
     2761 ggtatagctc cacagtttat agctataaaa ggtttgtctc tccttgaact atacttatat
     2821 attgttcttg ctattatttc ttttcctaca cctgattctc catttataaa tatagaagaa
     2881 tctgaggctg cagctctctt aacaactttt tttatattgt ccataatctt actttgactt
     2941 atatactctg tgtcttctga tatatcacta tattttatct gagattcaag cttaataacc
     3001 cttgaaatat ctctccctgt tagtactcct cctgtaagct ttcccttaga atcccatctt
     3061 ggaactgccg tatatgcaat aagcttccca tctgggtaaa cgatatttct ataaatcttt
     3121 ttcttcactt taagcatgtt taatattatt ggttcttcaa ctaatccttg tctcacaagg
     3181 tctgttatat ttctaccaag tacctcagtt cttttaaaag gtaatatctc atcactgaca
     3241 gtgttcatct tttcaattac cccatctttg ttaaacacaa caacaacttc atctatatgc
     3301 tctattatat cttcaattac tttatcagat attagagact tctcaaataa ataaactata
     3361 ttattttcat tattattatc tttcatattg tatatatgtg cagtcatcct taactctctg
     3421 aaagctattt tctttttact atctgtcagt atttctgttt tgtcaaaatt tgaatctaat
     3481 tcatttatat ttctaggctt ttttatatct agaaaactaa aaatactatc tattagatga
     3541 ttacagtata ttatttcgcc tgattcttta cataaaatgg ctggattttc tacatgttct
     3601 ataagcccta ttaaattatt atccattaat attcgcacct ttcattaagt aatttattga
     3661 actacataca aaatttattg tttgcttaca ataaatacca tcagcacata taatcaactc
     3721 cttataatat tcgtcttttc aagtagttta tttcattaaa acgtaactaa gatataattt
     3781 taatgattat agtttgttaa aaatttcatt ttgcttttaa ttgtatttta tcacatattt
     3841 agtaattatt tgtctattta atacaattta attaaattta agatttattt aaaaaaacaa
     3901 agataatcct tacagaatat aactcacaat ttgtaagcaa tatacatata aggattatcc
     3961 agattatctt gtattataaa attttattca aacagacttt ttaataactg acaaatacac
     4021 actagcaata acttatcaaa gggttaataa ttatgttact aactaataag acttattcaa
     4081 tcagtaatat ttatatagtt tgtatctatt aagcttttat agcttctaat aaatcatcaa
     4141 cgctatctaa tttttctata agcattggaa ctatttcatg taagtctcca actactccat
     4201 agtcagcgat ttctaaaatt ggagctgctg ggtttttgtt tatagcaatt atgaaatctg
     4261 atgattgcat acctgctaaa tgttgtattg ctcctgatat accacaagct atatataggt
     4321 ttggtttaac agtagttcct gtttgaccta cttggtgaga atggtctatc catccagcat
     4381 caacagccgc acgagaagaa ccaactactc cacctaattt gtcagctaat ttcttaagca
     4441 tttcaaatcc ttctggtcca cctaatccta aaccacctga tactataaca tttgcatctg
     4501 taagagatac taaatctttc ttagttttaa ctgtttctaa aacagtagtt cttatatcat
     4561 cttgagttat tttgtagtct aatgctataa cttcaccagt tcttgtttcg tctttttcag
     4621 ctttatccat aacaccaggt ctaactgtag acatttgagg tctatggttt ggacaaatga
     4681 ttgtagccat tatgtttcca ccaaatgctg gacgagtttg ttttattttc ttatcttctg
     4741 ggtctatttc taacttagta cagtcagctg ttaatccagt tccaactctt gaagctattc
     4801 taggtgctaa gtctctacct atatgagttg ctccgaaaag cattatttct ggttttattt
     4861 catctattgc atcttttatt acttttgtat atgcatcagt tgtatatttt tctaaaagag
     4921 catcatctgc aacataaact ttgtcagctc caaaagtgat taattcttta gctaatccat
     4981 caacatcttt tcctaataat attgcacaaa gttctgcatc tacttcttta gctaattttc
     5041 ttccttctcc taataattct ataactactg gagctatttt tccttctctt tgttctgcaa
     5101 atatccatac gtttttataa gaacttaaat cttttatatc attcatagtt tgtactcctc
     5161 caacttatat aatgtgcttt tgttttagac cgattattat tttactagct atttcatcag
     5221 ctttaccttc taagatttct ccttttcctc ttggttcagg agtaaatgat ctaaatactt
     5281 tagttggtga tgcttttaaa ccaacttctt ctttatttac atctaaatca tcaattgtcc
     5341 aaacttttac atcatgttta tatgctttta ctattttatc aacagacatg tatctaggtg
     5401 tatttaactc ttttactgct gttaaaagta caggtgtact tacttttatt aattcgtatc
     5461 catcttctaa ttgtctttga actattatat cttttccttc tattttgaaa tcttgtacat
     5521 atgtaacttg aggaatgtct aatttctctg caatttgtgg tccaacttgt gcagtatctc
     5581 catctattgc ttgtcttcct gcaaatatta tgtcatagtc acctacttta gatattcccg
     5641 ctgcaatagt atttgatgta gcccaagtat cagctcctcc aaatgctcta tcacttaata
     5701 atattgcatc atcagcaccc atagctaaac actctcttag cataaagtct gcttgaggag
     5761 gtcccattgt tataactgtt atagttacat catcatgctc atctttaatt tttaaagctt
     5821 cctctaatgc atttgcatca tctgggttta atatacttgg aactccatct cttataagag
     5881 tacctgtttc tttatttatt cttacttcgt tagtatctgg aacttgtttt acgcaaacta
     5941 atattttcaa agtctatacc tccaatttca ttagattatt tattatttta atacgtttga
     6001 tgaaattacc attttttgaa cttctgatgt tccttcgtat attgatacga ttctagcatc
     6061 tctgtacatt ctttcgattt cataatcttt tataaatcca tatccaccgt gaagttgtaa
     6121 tgctttataa gcaacttcat ttgcaatttc tgatgcatag tatttagcca tagcagattc
     6181 ttgagcaact ctcattcctg catctctctt ttgtgctgca tcataaacta atcctctagc
     6241 tgcacaaact tttgtttcca tgtcagctat agtaaattga gtattttgga atttagctat
     6301 aggcttacca aattgaactc tttgttttgt atatttaaca gcttcatcta aagcaccttg
     6361 agctattcct agagctaagg cagcaacacc tattctacca acttctagag tacccattgc
     6421 tatcttaaat ccttgacctt cttttccaag taagttttct tttggaactt taacgttttc
     6481 aaatattaag tcagaagttt cagttcctct tatacccatc ttatcttcat gagctcctgt
     6541 tgagaaacca tcccatttac tttctactat aaacgctgat gttcctctaa gtcctttact
     6601 tctatctgta actgctatta ctatagctac atcacagaat ggtccattag ttatgaaagt
     6661 ttttctacca tttaatatat agtggtctcc aactaattct gctgttgttt gttgtccacc
     6721 tgcatcagaa ccagctcctg gctcagttaa cccaaatgct cctattaatt cacctgttgc
     6781 tatacctttt aagtattttt gtttttgctc ttcagtacca ttgtaaagta ttggtaatga
     6841 agcaagagaa gaaccagctg taacaaaagt agctgttgaa gcacatcttc tagctatttc
     6901 ttccattact atagcgtaag aaacatatcc tgctccccca ccaccatatt cttctgggat
     6961 attactgctt ataaattttg catcagctaa ttttttgatt aattccttag gcattacacc
     7021 tgttttatcc atttctgctg gtaaagtatc taattcttta ctaacaaaat ctcttactgc
     7081 ttttcttaaa agttcttgtt ctttgttata taacatagct tcctccaatt ttcatatttt
     7141 atagtaaatt tattttaatg aacttacgct aaactcatca tctcagcaaa agtttgaata
     7201 cgagttctgg cttgttcatt attctgagtt tgttggtcga tttcaacata taaagtaggt
     7261 attccactat cttctatatc ttttctaact aaaggataat cgtattcttc tgggtcacag
     7321 aatttcatca tacagaagat aacaccatct atatctttct ttttaacttc atctactata
     7381 agtgacccac gttttttctt agggtcataa gctaatgaac atccttctat gtttgaccat
     7441 tgtcttgcta atctctctaa cgcatcatca cctgctggta catctgttct gaattgtctt
     7501 gtttcttgtg ctaagtcgtc agctacaact gatatattgt tgtcttctaa aatgtctaat
     7561 atatctttag aatcagctaa tatacctgtt aataaaactt tttttccaga acagacttct
     7621 tctggcatag catttaattt tgctattaaa tctttcacta gctcagtatg ttcttctttt
     7681 ctcataaaga acccactttt aattactaag cttcttattg atggttttat agtattagaa
     7741 tgtttataag ctacttcaac aaagtctctc atagtttttc tatgttcatt gtaaacttct
     7801 atactttcat gaatttttgc ttcttctatt tcatatccac aaatttcttc aagttctctt
     7861 tttacacctt tgtactcact gattaagtat tttacaccag cttcaagttt tctattttgt
     7921 gggtgtacta atgatatata ttttatatga ggtactgctg atttccagtt ttgtcctaaa
     7981 caaattagtg tatcacacat acctggtata ataactccag ataattcatc ataagcacct
     8041 tttaatccat attctaaaca tgattgcatt attgaacatg caaatgcagg gaaatattgt
     8101 ttagctaaat ctaattctgt ttgtcctccc catataccaa ctggaagcat tccagctgca
     8161 tgtataattt cttctgggca ataaactggg aaacaaccta tagctttttt accagtttca
     8221 tttttatatt tttttacagc cgcatttgga ttttcaacta cttctttcat tttagataaa
     8281 atagcttcca ttatatctca cctctattaa gtttttttct ttcttccatt acttcaacta
     8341 aaccttgaat tcttgtttca aattgagcat tagtgaaagc tctagggtct gcttggtcac
     8401 catcaaatcc agcatatggt aatccagttt cttcagctgc tcttctttgc atttcatatt
     8461 gtattaaact cataagttta cagcttctgt tcatatgata gaatgctcca tcacatttac
     8521 cctctactaa agaatcaact ctatattttg tcatacggtc taggtttaca ttgttaaaca
     8581 tagtactata tgctactgcc attccatcta aatcattaac ttcatattgt aatgcccaag
     8641 catgtgggta aacactacct gtcatgttaa ctccaaattt agctaatgtt ttcatcttgt
     8701 atcctatata tggccaacaa ggtatacctt ccatcattat tctgtatttt tcttcccctc
     8761 tgaaagaaga tttaccagtt ttcatgttgt cttctaattc ttctataagt aacttaaatg
     8821 cttctgtagt ttctttttta cctctagcac aaactattac agccatgtaa gtaaataagt
     8881 caaatccatt cattggagaa ggagaagaat ctgctggtaa actcatagaa tacttccata
     8941 gtcttccgtt ttcagctgat attttcatta cttcttcaaa cttcttaggg tcaaatttct
     9001 ttcctgatat aatttctagt tgttttatag cttcttcaaa ttgagcttta atataatcta
     9061 ttcttgattg agtaacttcg tcttcattat tgaaagttgt atcaatcatt attaaaggta
     9121 tatctaattc tcttgaaata ttttcatacc attttataac ttgattacat atattgttac
     9181 agcaaagtag gaaatctgga gctggcatat ccaaagcttc acatccacca ttttctaaaa
     9241 gaccaaaatt tgttcttgca tatgcacata ggtcaataga atatccttta gattcagcta
     9301 tttcacataa ttctaaagaa ccttttttag ctgctactcc agctgcttgg ttttctggat
     9361 ataatacgtt taagtcaaat acttctgcta actcttgagg aaatactgat gttgaccaac
     9421 ctacaggtct tccttcttct ttagctttaa atgcatttgc atattgttca gctaataaat
     9481 cattaattac tactctagct tctttttttt cagacatttt catccctctt tttatatatt
     9541 tttcacttct ttttgtgatt cttttgcttc atcaaaagca tataacgctg ctcctaaagc
     9601 accagttaat tgaggtatat caggtacaat aatttctgtg ttgatttctc ttgccatagc
     9661 tcttacaata ccactatttc tagcaactcc accaaccata actacatttc tttgtactcc
     9721 tattcttttt actaggctag aaactctctt tgctactgaa gtatgaatac ctgccactat
     9781 atcttcaatt tttgcatttt cagataaatg tgatataacc tcagactctg caaatactgt
     9841 acatgtactg cttattgata cttcattttg agaattcata gatatacttc cgagttcaga
     9901 tacatcaacc tctattattt ttgccattac atctaaaaat cttcctgtac ctgcagcaca
     9961 tttgtcattc ataagaaagt ttaatagtct tccattatta tctaatttta atacctttgc
    10021 atcttgacca cctatgtcaa taatggttct tgtctctgga attatgaaat ttacccctct
    10081 agcatgacag cttaattcac ttatttgctt atcagcatct gaatagttca ttcttccata
    10141 tcctgtaact acaacttttt taatatcttc tcttgcaaga cctgtcttgc catataattt
    10201 ttctaaaact cttgatggtc cagtagtccc agtaccagag gatattgttt cagaagctac
    10261 aatatcttcc ccattcttta agattactcc ctttgatgca gttgaaccta tatctaatcc
    10321 cattgtgtac atctttagca ttctcctttt aaaaaatcta gaattactaa ttaatatctt
    10381 acaactttac tatctttaaa gttatttatt ttctcttcat cgtagcctaa agattttaat
    10441 acttctacag tatgttgacc tacttttggt gctggtgtat attctttaat tccttcattt
    10501 ctaaacataa ctggagtatt aactaagaca cctgtatttc cgctatcgta tgttttctta
    10561 aataagaagt catttgccca agcttgttcg tcatctaata aatcttcaca gctttgaatt
    10621 ttttcaaatg gtaagtctgc ttcttctaat aaagctgacc actcgtctaa tgttttttct
    10681 aacatagctt ctccaactat cttaactaaa tcttcaacat gattaaccat tgaatctatg
    10741 ttattatatc tatcatcttc taatatatat tctctattta taaccttaca gaatttgcct
    10801 aaccacttgt tgtattgtat taaagctaat tgaatccatc ttccatcttt acatttatat
    10861 gtagtcatta atgggctgtt tggattttct cttgataaag gcatttcatt tccgtattgt
    10921 gctgttgtta tcattgttcc cattccatat atagctgtat ggaaaagact tactgttact
    10981 ctctcacctt taccagtttg agctttttta tgtaatgctg ctaaacttcc tgctgctagt
    11041 gctagacctg catagtggtc accaaatcct gctgctgtat ttgctggaga tgttcctttt
    11101 tccataacag attggctaac tcctcctctt gcgaagtatg cagtatagtc aaatcctggt
    11161 ttatctttta aaggtccttt ttcaccatat cctaatattt gagagaatat taatcctgga
    11221 tacttatctt ttatttggtc ataagctata cccatttttt ctaatgcttg aactctaaca
    11281 ttagttacaa atatgtctgc ttctgataat aatttatgta atatttctac tccttctttt
    11341 gattttacat taatacttac acccttttta tttccatttt ctaattcaaa catagggttt
    11401 tcatcatctg atgctggaga tttaaatgtt ccacccataa ctcttattcc atcaccttct
    11461 ataggttcaa tcttaataac ctctgcaccc cagtcaccta acatttttgc acaacatggt
    11521 gctgcgatga aacttgaaag ttctactact ttaactcctt ctaaaagcat aacgttttcc
    11581 tccttaatag tatcaattat aaaatttcga ttaaatcact cataaaatac ttttttctga
    11641 tattaactgt ctgatgtcaa agttcctaat cttatctaaa ataacttaaa aattattaaa
    11701 attatttttt attgttttat tgtcttacac ctatattgtt aagcatttat cgtgccaact
    11761 ttatatttaa tcaaaatatc gcttgatttg aatgtttttt aacaatatgc aattgaataa
    11821 tctttttcat atcgtggcat cttgccacta tccattgcat tttgctattc tttctatgct
    11881 tataaaagtt ttttttatat atttttttat gaaaaagctt gacatttcaa aaaaagtttt
    11941 cattgctaaa ctcaaaaaca catgttatca ttgtaattaa tcagcaagtt agttaactaa
    12001 tttcaaactt agataatttt ttaataatat ttaatctaaa aaataacaat ttctttgata
    12061 aattttagag taattattac attaaataaa aaattactaa atatcaaaat aacacaggga
    12121 aaacaatttc aaaactaaat ttttaacctt aaaaattttg tataaaaaat ataaccttat
    12181 atgctacata ttatttctaa atgaaaaatt accataataa tttaataatt acaggagggt
    12241 attgatatga aaatactagt atttggagca cgcgattatg aagaaccagt aataaaaaaa
    12301 tggtctgaag aacataagga tgttcaagtg gatatttatc ctgaaaacat gactgaagaa
    12361 aatgtagtta aagctaaagg gtatgatggt atatctatac aacaaactaa ctatatagat
    12421 aatccttata tttatgaaac tttaaaagat gctggggtta aagttatagc ttcaagaact
    12481 gcaggggttg acatgataca ttttgattta gttaatgaaa atggacttat cgttacaaac
    12541 gttccttctt attcacctaa tgcaatagct gaattagctg ttactcaagc tatgaacctt
    12601 ttaagaaaga ctcctctagt aaagaaaaaa gtctgtgaag gtgattaccg ttggatagct
    12661 gaacttcttg gaacagaagt tagatctatt acagttggtg ttataggtac aggaaaaata
    12721 ggtgctactt ctgcaaaatt attcaaaggc ctaggagcta atgtaattgc atttgaccaa
    12781 tatccaaata gtgatttaaa tgatatatta acttacaaag attctttaga agaccttcta
    12841 aaagaagctg accttataac attacatact cctttacttg aaggaacaaa acatatgata
    12901 aataaagata ctctagctat aatgaaggat ggagcttaca tagtaaatac tggccgtggt
    12961 ggtttaatta atacagggga tttaatagaa gcactagagt caggaaaaat tagagctgct
    13021 gcccttgata catttgaaac tgaaggattg ttcttaaaca aaaaaatgaa tcctggagaa
    13081 ttaactgacc cagaaataaa taaacttctt tctatggaac aagttatatt cactcatcac
    13141 cttggtttct tcactagtac agcgattgaa aatatagttt attctagttt aagtagtgct
    13201 gtagaagtta taaaaacagg aactgctact aatagagtaa attagtattt tataaatata
    13261 ttttttcatt aattagctat tatctaacaa aatagccttt aatttcccaa atataagaga
    13321 gtcagctcag tatatattga gtagactctc ttatattatt ttttatatta aacttgtact
    13381 cctcttattt ataccaaatt aaatttatat taacttactc taactactcc tactctatac
    13441 attttaccta tttttacttt tctaattata tacatttgaa tgggtacaat ctgtatttta
    13501 caaaaaaaac acctaacaaa ctatgaaaat tgtatgatta atattaatat atttataaga
    13561 aaacataaca attatgttag ctctggattt ttagggaggg agtatattgg aaaaaatgaa
    13621 taaagttact tctaaaaaaa ctacctatat tgttacttca gcattatttg catccataat
    13681 atgcctgaca atagcatata tattacatat tccagttggt ggaaacaatg gatatgttca
    13741 tataggagat gcttttattt atctagctgc aactattttg ccaacaaact atgctatagc
    13801 tgcttctgca attggtgctg gtctagctga tttatctaca ggtgctgcta tctgggtaat
    13861 accaactata atcataaaac caatacttgt attatttttc acttctaaaa gtgacaagat
    13921 aataaataag agaaatatag ttgcttcggt tgtagcaggt atagtaggat tagttttata
    13981 catgtttgct gaaggtataa ttattggaag ttttacaagt gcatttgtaa tgagcttact
    14041 tggcttatta cagccaatag gaagttttat tgtttttata atcttaggta tggcacttga
    14101 taaacttgat tttaaaaaac gttattttaa ttaagaattt aaatttatgg agggaaaatt
    14161 atgaatatct tatatacaat tgaaaatagt atctatgtaa atataactaa tacatgtcca
    14221 tgtagttgtg ttttttgtat aagaaacgaa aaagatgaag ttgcaaatag tggtagttta
    14281 tggttggagc atgagccaag tgtagatgaa gtaaaagaag ccttcaataa atacaattta
    14341 gacgactatg atgaaatcgt attttgtgga tatggcgaac cattaatgag aataaatgaa
    14401 ttgatagagg tagcaaagtt tataaaagaa aaaagtagca taaaaataag aataaataca
    14461 aatggtctaa gtgatttaat acataacaaa aaaacagcta ttctgttaaa agatgttata
    14521 gatacagtat caataagtct taatgcccca aataaagaag cttataatag agttactcaa
    14581 ccaaagtttg gagaaaaatc atttgactat atgttaggtt ttgcaagaga ttgcaaaaaa
    14641 tatataaaag aagtagcttt ttcagtagta gatgaaatat ctccagaaga aatagaagaa
    14701 tctaaacaac tggcaaaaaa acttgatata ccattaagag ttagacataa gaattagcaa
    14761 gtaaaattat aaaacattaa gaatcacctg aatggtcttt caggtgattc ttcctcttgt
    14821 attaacattt tacaaaatag atttcatacc acactaggaa tgtgtaaatc atccaaatct
    14881 ttctccaatt atcagagttt ccactaatgt agtcattaaa aatctcatta atctcatcta
    14941 tgttaaagaa tttatctgct atctcactat tgaatttatc tcggatatcc ttattatatt
    15001 ggtcatcagc catccatata cgaattggca caataaatcc taatttattt ctaaaagcaa
    15061 tctcttctgg taatgttttt gatgcagctg tacgaaaggc aattttattc tcttttcctt
    15121 tcactttata ttctgatggt attctggatg caatatcaaa tattcttgta tctgtaagag
    15181 gcattctgac ttccaatcca actgcagtac tcattttatc tatattaaaa taaatatctc
    15241 cttccaacca tatttgaata tctacatttg acatgttagt aagtgtatct agttcttctg
    15301 tttcttcata aattcctttt accacttcaa taggaagcac atcaggattg tatttcttaa
    15361 gaatttttct tttctcaccc tcattcataa tgtttgtatt tccaatataa aatgtcttca
    15421 aattctctcc ataacactct gccttatggt atatattata tccaccaaaa aactcatctg
    15481 ctccttctcc agagtaacag atatttgtgt tttcagctgc tgccttgcat ccaatagcaa
    15541 aaacgatagc agatgcatct ccagttggtt gctccatatg gtacattaca taaggtactg
    15601 acgcaaaata ctcttcaggt gtaattatat atctcgaatt tttacaccca agaatattag
    15661 ctgtctgttg tgctaatttt gattcatcaa aacgctcttc ctcataacca catgaacctg
    15721 ttttttctat atctgacatt gccaatatat aagatgaatc aacacctcct gataagaata
    15781 actctgcagt ttcattctct gattttacct ctggcataat tttctgaagt gttgtatgaa
    15841 tttcatccgt ccattcctca atagacttgc tgtggtcagg atgaaattca ggtttccaat
    15901 aacgaccaat acatactgtt ttattctgat agacaaggta atgtcctggc ataagcttct
    15961 ttactccacg gaagaatgta ttttcacctg ctacatatgt gaaagacata taaatctgta
    16021 acatgtcttc atttaactct ttcacaaatc ctggttgctc cataatctta cgaatagatg
    16081 aaccatagag aagcttccca ttttcagtct cataataata gaaaggtttt actccaaact
    16141 ggtctcttag gcagaagagt ttcttttcca cagtatccca taatgcaaag gcaaacattc
    16201 catgcatgtg atttgccata tcatatcccc atgtcttata tgcttccact aaaattgctc
    16261 gttcacgctc atctctacat aatgaagaat cgattccaag ttcttcacag agaactttcc
    16321 agtttcgaat atgtccattg tattctagaa tttttatcat atatgtgtct tttcgtgtta
    16381 aatacttttc ttctaaattt atcatatgtg tatcctttcg tattaaaaca tttaacttct
    16441 aaatttatta aatgtttata tatatttatt cacttataat cactgaaaaa tcgacttttt
    16501 tcaaagtaaa ttctttttga attagtacta atctgctctt tattaatggc ataaatatga
    16561 ttatcaagta ttatcagtgt tataacattg aattttttat tcttcataaa tattgccccc
    16621 tttatattac caactaaata taatatatct tatgttactt acatattc
//
LOCUS       DAMJVX010000077        16581 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMJVX010000077 DAMJVX010000000
VERSION     DAMJVX010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.080"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(229..1515)
                     /locus_tag="PC407_002174"
     CDS             complement(229..1515)
                     /locus_tag="PC407_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2497618.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAISENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(1638..4736)
                     /locus_tag="PC407_002175"
     CDS             complement(1638..4736)
                     /locus_tag="PC407_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2497619.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGINDISHVK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKFKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFIVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMEVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFDDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDILTGLYN
                     YESFCEQTSLMLKRYPDKSYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDVHKLIYKIKNSLNRKKFEYMLVPCFGVYKILDNN
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLRHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            5460..5612
                     /locus_tag="PC407_002176"
     CDS             5460..5612
                     /locus_tag="PC407_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497620.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHYPAFAGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(5904..6608)
                     /locus_tag="PC407_002177"
     CDS             complement(5904..6608)
                     /locus_tag="PC407_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2497621.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(6599..8206)
                     /locus_tag="PC407_002178"
     CDS             complement(6599..8206)
                     /locus_tag="PC407_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HDF2497622.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(8460..9638)
                     /locus_tag="PC407_002179"
     CDS             complement(8460..9638)
                     /locus_tag="PC407_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497623.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(9864..10358)
                     /locus_tag="PC407_002180"
     CDS             complement(9864..10358)
                     /locus_tag="PC407_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2497624.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(10359..11120)
                     /locus_tag="PC407_002181"
     CDS             complement(10359..11120)
                     /locus_tag="PC407_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2497625.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            11843..14677
                     /locus_tag="PC407_002182"
     CDS             11843..14677
                     /locus_tag="PC407_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2497626.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHVSIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNISKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNIRKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            14720..15964
                     /locus_tag="PC407_002183"
     CDS             14720..15964
                     /locus_tag="PC407_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2497627.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKV"
     gene            complement(16405..>16581)
                     /locus_tag="PC407_002184"
     CDS             complement(16405..>16581)
                     /locus_tag="PC407_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HDF2497628.1"
                     /translation="TYKTAIDEAQAILDKADATQKEIDDAVTALNTATATFEAGKVPT
                     TIALMLSRILGFMK"
BASE COUNT         5777 a         2738 c         1891 g         6175 t
ORIGIN      
        1 tttttaataa caagttctag tatttttata ataatttgtt ctagtatttt taataacaag
       61 ctttagtatt ttacataata atttgttcta gtatttttaa taacaagctc tagtatttta
      121 tataataatt tgttctaatt tttttcatga taagctttaa tactttatat agtaatttgt
      181 tgtagaattt tataaaatat aatgatttat acagttttat aaaatatctt aaaacagtat
      241 ttcagtaata ttttttggtc ttcctccacc aatactagtc tcttttgcac atattcgacc
      301 taaacctgca tttactattt tatttaatat acgtcttgca cttctttcac ttacatttag
      361 atactctgct aactcttttg aatcataaat cttacttttt ctattctcac ttatagcaat
      421 aatttttgca actgactcac aactcattcc agtcttttta gatatttcta ttaccttttc
      481 atcagatagc atcaaagaat aatttaattc attttcggaa gctaaaggtc ctcttatatt
      541 ttcatcttca tctaccaaat atataccaat ttcttttgaa tctatggagt gtcttaatgc
      601 tttatgtgca ttattttcag cctgatacgc agttactcca gtaccaattc ctatactaag
      661 agaaaagccc atgtctttta tctctctctt tagattgaat agcttatcat aattatactc
      721 attatcagct acccctttat gaacaaatac aacataatca tttcttccaa gtaaaaataa
      781 ggaaccttga atgcttctta catagtttac tacaagctta tccatatctg atttttttat
      841 catgtcagaa taatagttat ctatcttctc tttataatct attagactca atatttcgac
      901 agcaatttga gaaaatcttg ccttgcttag agcatattca ctctttacct tatcataact
      961 ttctttgatt aattgtatag tagcttgtaa acgaaataca ggatagcctc tttcttttaa
     1021 ttcattatat actgcaccaa aaccagttaa cattatatcc gtcttatttt cttcaaataa
     1081 ttttatatgc cattctacaa gttcagactc tcctacttcc aaagaaaatg gattacaaaa
     1141 tacactcgtt gctttaatat caaattcttt aagtgcatct tccacaacat atctatcaac
     1201 tacatctata ctgaatttat ctaattttat atttgcactt tttattgccc aaaatgcctt
     1261 cattatactc gtcccacttc tcggtacaaa tgaatgtggc ttacttatct catacttaga
     1321 ttttataaat tcataaactg cacatcctgt aaatataata gcatcacatt catcttcaca
     1381 tttagaaata acttctattg tatctactac tttttctctt acatatattt tagtctttaa
     1441 atcactgtct atctgtttta aatcatcctt tatcttaaac ccagagtctg aaggacctat
     1501 aatacccact atcataaact tcgctcctaa tccataaata ttatactttt taaaaattat
     1561 atcctcacta ttttctactt ctatttaatt atgttgaact actccatcct acctacattt
     1621 agagtgggat aattagctta tttttgataa agtaacttct caaaatcagc acttggaata
     1681 ggcttacaat aataataacc ttgaattacc acacaatcta aactcactaa aaattcagct
     1741 tgttcttttg tctctacacc ttctgccact acatccatat ctaaatcttt tgccatggct
     1801 atagtgtgcc ttaaaactat tttttctctg tctgtgattc cttcctcatt aaagaagcca
     1861 caatctatct tcaatgtatc aatattaata ttttttaaca tatttaatga agaatatgct
     1921 gaaccaaagt catccatatt taaaacaata ccatccattc taaggtcggc tattatctta
     1981 gtaaataatt ttatattttt aataaataaa ctttctgtaa gctccaattc taaaaaagat
     2041 tttggtatac tatatttatt tattaaactt aaaacaattt ctttaaaatg tggattatgc
     2101 aaatgtaatc ttgatacatt tacagagata ggaactggct cttcacctaa atctatccat
     2161 tttctaatca aagaacatac ttgttcccac acatacatgt ctaatctgat aataaaccca
     2221 ttcttctcaa atagtgaaat aaatttaatt ggaggtatta tacctttttc tggatgaacc
     2281 catctaacca atgcttccgc accaacaatt ttatttgcat taagattata cttaggttgt
     2341 agatatacat ggaattgccc attttcaagt gcgtattcca tttccctttc catctgtttt
     2401 tcttcaagtg catcatttct tatagtatca tcataaaata tatagttatt gatactttgt
     2461 ttctttactt ttttgtgagc atatcctgcc atttcacacg catttgtaat tggaatatta
     2521 ttatccaaaa ttttataaac cccaaaacat ggtactaaca tatattcaaa tttttttcta
     2581 tttaagctat ttttaatctt ataaattaat ttatgaacat ctctatcaga gtaactctca
     2641 taaaaaatac aaaatacatc tgcatacata cgacaacata taccattaga accaatgttt
     2701 ttttgaatca attctgcaac atatattaaa gccttatctc ctcctctaac tccataaatg
     2761 tcattaatta ccttgaactt atctatatcc atcattataa ttgcatagct cttgtcagga
     2821 tatcttttaa gcattaaact tgtttgttca caaaaacttt cataattgta tagtcctgtt
     2881 aatatatcct tttggttttt atgtagttgc ataagataat ctatattttt tatagcaact
     2941 aaaatatgtg tttgtttctc acctaactta tttttactac taatctttaa catataaatt
     3001 aatgcccaat agtacttttt atctattcct aaacgtctat actcaaaatt tagttctaaa
     3061 tcgtcttgct tgctgttatt aagactatca agagatttta gaaggtggtt atcatcaaat
     3121 aatattatat catcaggatg aattaaatgt ttttttatgt atgaaaaagt tttttcaaga
     3181 ttttccttta taggaaaact aaaaatattg ctctctgatt gataaattgg agtaattaaa
     3241 ttagtatcga tgcaaataga taaaattata tcatatgtat tataagctaa tttttcataa
     3301 gccttagaat acccttttat cattaaatca tcaaaataaa ccttatcaac taactcttta
     3361 tgtttaaata aaatattatt cgtcgaatca gctaagtcac taattacttc catttcatta
     3421 tttctaagtt tgttcttatt ataatacctc ttcttatctt ggtacattct ctcatcagca
     3481 actttaatta gcttatgtat atcaatatta tcgtcactcc aaatactgcc cactgctaca
     3541 ctatatatca tctcatcctt aaattcttca cttaaccaac tcactaattc ttcaaatagt
     3601 tgttttgata tatcttcaca taatacaata aactcatctc ctccaatacg gtaaacatta
     3661 taagaatgaa agtatttttt tattattaca catgctgaca caatagcaga gtcgccatta
     3721 gcatgtccaa agttatcatt aatatctttt aacccattta tatctacaaa tacaactcct
     3781 atggatttaa attttccttt ttgaaaatta tctatatact taatatattt gtttctatta
     3841 tatgcaccag tcaagaagtc atagtagctc atataattta atttattttc tacttttctt
     3901 tttttcatct cagttaataa aaaataagat agggatgtca aaagagaggt atctccaaca
     3961 gccttatgtg gattatcaac tccaataaac cctactactt tatcatcttg ttttagtgaa
     4021 gatgtcatta aactttcaat tttttgaggt tttaaaattt catattctat tggctgttct
     4081 tcttttatat tttctatatc atcaattatt acaaaaccct tttgttcgaa taataaaaac
     4141 caattcttta attcattaat atcaagattt gttagtgcct gtttttgttc atcaacacct
     4201 tcattgcacc attcatatgt attagttact ttttttctat cttctgatac ttcaaaaaca
     4261 tatgctctat cagctttatg aaatttacca atattggtta atacaagatt tacagcatca
     4321 gaaaaattag attctgttat aagatgttta atacatccaa ctaataactc ctttatctct
     4381 aatttttctg ctattgactt acttatatct tccttttctg taatatcaag agctatttct
     4441 aatcgtgcat gtttgccctc ccatttaatt aacttgtcct ttaatataaa atgtctattt
     4501 gttcgagtat tcgtattttc ccatatgtaa tattcatctt cttttaaaat agagtttgta
     4561 caaaattcgc agggagaatc tttcccctgt aatgctttat aacattttac atgggatata
     4621 tcattaattc ccattgcctt tttcccaatt tcattaatat ataaaagttc atatgtatct
     4681 aaagaagaaa catatacaat ttcatccaac tcatctatta aatttattac atccatttat
     4741 atatatctcc ttttattcat tttccagatt gaatttttaa agcttttaaa attttgctat
     4801 atatttggca atgatattat acttctttat gttttaaaat agtaccttaa taaagttcta
     4861 ttttttagta aatagaatta ttattgtgtc aatcgtacaa tagagttttg taatacctaa
     4921 tttaatatat ttataattaa ctatcttatt atgtgtttac aattaattct catataaatg
     4981 acaaatctcc acctcaatta agtattccaa tcatatatga agataatact tcattcattt
     5041 aaaaatataa aactattata tattaaaatt tcttcaataa taaattatct agattgttac
     5101 tttgatatat tatataatat cacaaaggct gatataaaaa gacaaaaagg gtaatttttt
     5161 tatatcaaaa aaaatgttaa attgatatta tccatatata aattgatatt atccatatag
     5221 atgctatatt taacaattct acttacttcc cttacactta ttagcatata gttattaaga
     5281 gtttgttgca aaacaacact tagcacactt taaaaagtgt ggtctaataa gtaatcaacg
     5341 aagaagacat aataaagcat aaaatatgat atacttttcc tgtcatcaaa actgatgatt
     5401 ttactattaa tatctgctaa aatatgctta ataaattaaa ttttagtggg agaatagtta
     5461 tgaaaatatt tagtttcttg ttgtttgtag tgggaatatg tttttgtatt gctggattta
     5521 tgggacatta cccagcattt gctggtgcaa cattgagttt ttttgcaact acaatgatag
     5581 ctgtaagaca agtcaaaact aagaaaaaat gaaataggca aatcctaact ttacaagtta
     5641 atattacata agtaattagt ctaaaattgg atactttttc tattacagaa atacatcatc
     5701 cactttgtat tctcttaaaa aaataaaata gctatgtaaa ttcaagatta gatagattaa
     5761 ctctctatcc atttcattgt aaaatataca tagctatttg tattcgattt aaaatactta
     5821 gattttctta cataacaaat aatttctatc tgattattac tcatattcat ttagtaatca
     5881 ctcagcaact aatcatttag tatctattca tccgccatac ggtaacctac cccaacctca
     5941 gtcaaaatat attctggctg agcaggattc ttttcaattt tccttcttat acttgccata
     6001 aaaactctaa gcgactgatt ttcactttca tagtaatttc cccaaacttt atctattata
     6061 aacttatgag ttaagactct tcctgaatac ttagacatca actcaataat cttatattcg
     6121 attggagtta aatgtatttc ttcaccatta attgacacaa tatgattttc ataatctatc
     6181 tttaggtttt taacttcaaa agactttgga gcatcattat ttatattatc ctgttgagct
     6241 ttatttctaa gagccacacg tactctagcc aaaagctctg ttacactaaa aggtttagtt
     6301 aaataatcgt ctgctcctcc atctaaagct tccaccttat ccctctcatg ctctctcgct
     6361 gaaacaacaa ttattttatt agtctttgca catgccctaa ctcgtcctat tacctcaatc
     6421 ccatcaatat ctggtagtcc taaatccaaa attatcaaat ctgggtcaag agaatgtata
     6481 agtgatatag ctgttcctcc attatctgtc tctacacacc tataattttg agtttcaaga
     6541 gatactttta taaatttaca aattggctta tcatcttcta caattagaac taatggcttt
     6601 atttccactt gtctttcccc tcctcatcat acataggtat gctaaatcta aatgtagctc
     6661 ctccagaagg attattaaat gcgacaattt ctccaccatg agcttctact atagatttac
     6721 atatctccaa tccaagacca atacctcttc tttcaagatt tctagactta ttcttagtat
     6781 aaaacctatt aaaaatatta ccgatatctt catttggtat tccttttcca ttatctgcaa
     6841 cttcaaatag cacttgattt gactcttttt ttacagttag ttctatctta gaatttttag
     6901 gtgtatgcct tatagcatta tcaattaaat ttactaacac ttgttctatt aataatacat
     6961 ccacatgaac gattattatc tcatctggaa tattggtttt tatgtctcta ctattggcaa
     7021 actttttgac tcttaatata gattcagcta ttatttcatc aacaacttct ggtcgttttt
     7081 caatatccaa tttcccttca tctattctag tcatactaag tatattctca acagaatgag
     7141 ttagccaact cgtatcttca tatatatttt tcaataattc tactctagtt tctttatcta
     7201 tcacatcatc attttcaagt attgttgaag acgaacccaa tatacttgta agtggagttc
     7261 ttaaatcatg agaaattgac ctaagcaaat ttcctcttaa tctctctctc tctgcctcaa
     7321 gatttgcttt tttatttttc tcaaataaat tttctctatc aattgccaac gcaacctgtg
     7381 ttgaaactgc ttctaatagt atcttttcat tttctgtaat aatatctatc ttaaaacacg
     7441 caacacctat aacaccaaga acatttgatt cttgaccttt tatagggaaa taatatgcac
     7501 tgctgttttt gcaatatttt gtcccaattc caactgggtt acctgtccta aaagcttctt
     7561 ttatagcttc tttttctaat actgaatgga atatattctc acttccatca ttgttacaaa
     7621 tataaatact tggcttacct agattattat tctcatctgc aatagtcact attacagttc
     7681 tatttaatat atttactaaa ttagacccac aaaattcaat aatttgattt ttattttttg
     7741 ctttaagcaa cgttttgtta ttctcataga gcaatcttat cattttttct ctcttatgag
     7801 aaagtctcac ttctcttttt atcctagaag ttaaagtact tgttataatt gctgccatta
     7861 gcataataca aaaagtcata ggataatctt ttctataagc taaaaaagta tataatggat
     7921 gcgtaaagaa aaaattaaat gatagcactc ctattactga agaaagtatt ccaaaaaaat
     7981 atccataggt tatacttgta gcaaatagta cacctaatat gtatactagt ataatattag
     8041 attcattaaa acctaccttt tcaaaaacta ctgatattcc agtagcaatt gtcataacca
     8101 atatagtttt tatgaagtcc ttaataatta tctttttact gagatagcca tcttcatcat
     8161 aatcatactc ttttaagtct tcatgttgta gtcgttcctt tttcatttac tacaattcct
     8221 ccttattgtt actgtttaaa ttggctctat tttatataat acaattttat cataataaat
     8281 tcaatcaata catttaatta atgtacttaa attactaaaa ggtgctgtct taaaatgaaa
     8341 ataaatctct atttcaaaac agcacctata tatttaaatt actaatttag tttttacata
     8401 cttaaatcaa tagttttatt caattttata aattataata cttcaattat ggaacatttc
     8461 tatccaacaa gcataaatat aaagtgggca catatacctg cacacaaacc acctatggta
     8521 tgcgaaagta tcgctttacc agccaatggt cttgcatcta aagcatccat catacctatg
     8581 tgtgtactta aatatccact ccaacacata cccattgctg tgaatactgc tatatcatta
     8641 ggagttatag cgcctgattt tataaactct ggtactaaag atattgctgc tccaacagct
     8701 cctaatgcag ttacagggaa tgctattgcc tctggtgatg tgaatccaaa taatggttct
     8761 attatgaagc taatcttatc accaatagca ggtaataatt ttataccttc atatgcagct
     8821 cctgtgtaaa cagcttgacc tgtagcaggg tctgtagatg gtccaaaagt taataacata
     8881 actaatgtac aaactactaa aactcctggt attatagcca tacccatttc aacacctagc
     8941 ttaccacctt caagtatagc atccaatgtt ctttggaata cattaccatc tcttatttct
     9001 ctaaactctt caagttttgc acctttatca tttatttgct tttcagtatc atttttaggg
     9061 tcataattat aatacttttt agtaaaagtc aacataattc taacactaat aatactacct
     9121 attattgccc caacattacc tattatagct ggtagtacat attctttacc ttgagctatc
     9181 ataaaagttg ttacaataag tcccatacca aaggctgttc ctaaattaca taaagcaggt
     9241 acttgatact tcttaaaata ttgtgtaaat gttttgtctt tagcaaatgg tataatagct
     9301 ggattatcag ataaatatgt tgcaactact cctgctatac tagcaccagg taagccccat
     9361 ataggtctca taagaccctt aaatatctta ttaactaaag atattactcc aaactctgaa
     9421 agcaacgcac taagtgcacc tgcaagaact gccatagcca ttattaaaaa tactgtgtct
     9481 aataaaagcg catgtgctgt actcattatt actttaaaca tcattcctgc acccatgatg
     9541 cttcctacat atccaaatcc taccgccaat agtacaataa atacgaaggt ttccatactt
     9601 actgccttga ctcttctctt atccgacagc ttttccatct tgaaatcttc ccccttttaa
     9661 ttttaaatta aggttcctaa cattggtaca aattttttgg tacaaactga gttctaaaac
     9721 ttacactcgt aatgcaagtt gtcagttttt tgaaataaaa aaaaattacg tttatagaat
     9781 aagaatatcc tctttaattt ggatactctt attctatttt atgttattat attatcatcc
     9841 ttttgtttaa aagtcaattt tttctatttt tgttgtttaa ttttatttat actcttttta
     9901 tatttttcat tttcttttat ctttgtaagc actccatata agttgacttt tctaaataca
     9961 gtagttataa ataccaatcc taatgcaatt gaacttgcac atgataatgt actcacccca
    10021 tattctacag cttttatata attattggtc aaaacagaat acatagtatt atatattcca
    10081 taaccaggta ctaatattat taaacaacaa acacttaatg tagttgctgg agtcatataa
    10141 attcttgcac ataactcaca atatatcgca aaacaaactg tagctataaa aaaacataag
    10201 ttttctgtta ctcccatatt taaaagaaat ttatatgaaa accaagctat acttccacct
    10261 attcctgcta gtattaagtt ttttcctttc atattaaata gcactccaaa gcttaaggca
    10321 gtaaaaaaag ctgctattac ttctataaac aaatgcactt atatcccccc taacttgaag
    10381 tataatccaa gtaccaagaa cattcctact gctatagata cacctacaaa aaatacttct
    10441 gctaattttg caagaccaga aatcaactgt ccatctaaca aatctcttaa tgcattagtc
    10501 aaagcaagcc cgggaactaa gagcattatt gctcctgcta ttaaatggtc taacgtcttt
    10561 ataaatccga gctttaaaat taaaattgaa caagaagcta taataactgc tccaattgca
    10621 tttacaaaaa agtcattaag tgaagattta cttaaataca taataacaaa cttagtcaga
    10681 atcccaacag taaatgcagc tataaattct tccacatttc ctccaaataa tatcgaaaaa
    10741 gttgctgctg caatacctgc aaaaaatatt gttattaaat ccgagtaata gttatcttca
    10801 cttatttcat ctaaaatttt ctcacataat tcaatatcta aattatttat acttattgct
    10861 cttgataatg agttaacttc atgaaccaag tttaggtcta ctcttcttga gcttattctt
    10921 ttaattgctg aatgtaattt cccatctaca aatacagatg ccattacagc tgttggagtt
    10981 gcaaatacat ctacgtggtc tattccaaaa gattgacata ttctagctat cgtttcttca
    11041 actctatagg tttctcctcc actttgcaac atagctttac ctgcatttga ggaaaagttt
    11101 aaaattctat ctatatccac aaaacatatc tcccattctt ttatagtatt attcatattt
    11161 taatacaaga tattatttat ttaaactatt ttaaataatt catatccaat atttacattt
    11221 tatgaaaaaa cttgtttttt atgcttgtca aaatcaaatt ttaagcttgt ttacaccata
    11281 aaatggctga attaatattt gacaaacact ttcaaccatt tatataacct tctaaattgt
    11341 ataacttttt actacaaata aaattatatc tcttttaatt ctttctcttg agtatttaag
    11401 cttatagcaa cttcatattt atatatgtac aatataaaac attattttta tgatacaaaa
    11461 tataatttgt aaacgtatat atgtattaac tgtgtatata taaattattc ttaaccttat
    11521 cagttttttc atatatcttc atctatatcg accatatatt aattacattg accatatact
    11581 aataaatgat attacataga ctgaagtata ttaaaaaact taaatttagt tatttaacaa
    11641 cataatactc taatttaatc tataaaaatc catacctaat ataatgcaac tcttatttta
    11701 acaccatcta ttacacaaaa caacatatta aaaagtaaaa tacaaaatat ccactataaa
    11761 ccttatttta atactgaata tatattataa tatatttaat gtagcttaaa tatagtttaa
    11821 acataaagaa aggaaatggt atatgctaag aaaagataaa gttctattat ctctacataa
    11881 attatgtgaa aatatgactt taaaagacct tcaatcagat aaaacaggat tcactacaat
    11941 agaaattgct aaccattcat ctcttgatag aagtaatacc agtaaagaat taaataacct
    12001 atataatgaa aaaaaagttc tgaaaatatc tggtaagcca attttatttc tacatgttag
    12061 tattatagaa aatttaattg gccgtacttt gaacagtgat gaattttcta taaataattg
    12121 taatgagcta cttaaatcaa gtaaaaatga ctcagaagat attttttcaa cattacttgg
    12181 acatgattct agtcttaaac tacctataga aaaagctaaa tctgctatac tctatcctcc
    12241 aaaaggtctt aatacgttac tcataggacc tactggtgtt ggaaaaagta catttgccga
    12301 aacaatgtac aaatatgctg tagaaagtaa aatcttttct tctgaaagca aattcgtagt
    12361 atttaactgt gctgaatatg cagaaaatgc taacttatta ttaagtaatt tatttggtta
    12421 tgtcaaaggt tcttttactg gtgctaataa ggataaattc ggcatagttg cacaggctga
    12481 tggaggcata ttattcttag atgaaataca cagattacca ccagaaggtc aagagatgct
    12541 ttttcttttg atggacaaag gcatatacag acgattagga gaatctgaca ttgtgcacaa
    12601 agctaatgtc tttattgtgt gtgccacaac agaagatata cattcttctc tgcttgggac
    12661 ttttttaaga agaatccctg ttacaatcaa cctacctgct ttacaggata ggtctttaaa
    12721 agaaagatta tctcttatta aatacttctt tattatagaa tcaaaataca ccaatgcaac
    12781 tataaaagtt cacaaagatg taattaaatc attactcctt tatgattgtt ttggtaatat
    12841 aggtcagctt aaatctgaca tacaacttat gagtgcaaga gcctttttaa attacaaaac
    12901 tggacttaag gataatatag aaatagattt atcccttgtt cctgattaca tctatattgg
    12961 tcttttaaaa ttgaatgaaa atcgtagtaa tctaaataaa ttatcagaac ttgattatat
    13021 gcttttttat gaatttaatg gtaataactt agaagttgaa tatacaaaag aactatctaa
    13081 atacaatata gatacatcaa acaatggatt taaatactct acagagattc cttatctgga
    13141 aaatgatata aggagtatgt ttataaatta catggaaaag tactctacaa aattactttc
    13201 gatatcttca gtaactcatt ctgattatac aagtaatgaa atttttaaag taatcaatcc
    13261 aaagatttac tatgctgttg aacattcttt aaaagaggcc gaaaaaatat cgagaaacaa
    13321 atacacaaaa caaacttata ttgctctttc aatgcatata agtgcactta tagaaaatat
    13381 aaatcaagac acctataatc ctaagtacaa tttatataat gataaaattt tcttagataa
    13441 agatttaaat atatctaaaa tagttttaag tataattgaa aatgacttac aaacatcttt
    13501 acctaaagat gaagtaaaat ttatatccat gtttctaaat agcatgtatc ttgaaccaaa
    13561 caatcaatct gaaagtatag gagtcataat acttgctcat ggaaattcaa cagcaagcag
    13621 tatgtgtgat gtagcaaatt cattgcttgg tacaaaccat tgtaatgcaa ttgacatgcc
    13681 tcttgatgta aaagtaagta ccatacttga taaaactata gagttggtaa aagaatgtga
    13741 cgaaggtaag ggtgtacttc ttttaacaga tatgggctct cttatagctt ttggagaact
    13801 tatttcagaa aaaacaaaaa tcaaaactcg ctcaattgaa atggtatcta ctccaatagt
    13861 tttagaagca gtaagaaaaa gtacattacc agaaatgact ttggatgaat tagctaattc
    13921 cttaagctct ttaaatccat atattggacg agcagcaatc gattcaacag aaataaacta
    13981 tcctgaaaag tcaagagagt atgtaataat aactacttgt ataacaggat atggttcagc
    14041 tatgaaagta gcagacttta taaattcttc acttaaaaat attcgtaaat acaatattaa
    14101 tttagttcct cataatatag aaagttttaa aatgtataag aataaattta gtaatactaa
    14161 aattctggca gttgttggaa gtgtagattt taatgtagcc aacacccctt ttatacacat
    14221 agaggaatta ctaaatggat atggcctaga tatgctaaac aaaataatag aaggtacatt
    14281 caatgccaat agtattacaa atataaaaga aacaaatcta tcatacaaca gtttaataat
    14341 aaactttatg acccaaaact tagaactttt ggactctgtt aaattattca actatgtcaa
    14401 ttcttcctta tttacaataa tagaattaat agatgaaaac ttaaagagca aatttaagct
    14461 aggatatata ctacattgct cttttatgat agaaagatgt tttaaaaata caacttttcc
    14521 atataataac attactgaat taattagtaa aaatcatgaa ctatatagtt taatacgtga
    14581 agcctttaag ccaacagaag cttattttaa tattttgata ccagacgaag aaattgctta
    14641 tataatagac atgattaatg agtatctaaa taaataaaat ttatagcact tattttgtgc
    14701 atattaatta atatatgaaa tggaggacga gattttgaaa ggaaaaataa aacagttaac
    14761 aatacttgca ttaattttta tatttataac tcctgtattt gcatttgcag atactccacc
    14821 agtaccaaat tcttcaagag cagcactact tatagaccag gaaactaaaa gaatattatt
    14881 tgaaaaaaac attgatgaaa aaatgccatt agcgagttta agtaaaatga tgaccttttt
    14941 attggcaata gaagcagttg ataagaatca agtaaaagag actgatatgg tgaaaataga
    15001 caagtctaca gcttcagtag gaggttctac ttgtaaactt aaagatggtg atgaaatatc
    15061 tctaggagag cttatgcagg gtctaatgct agtatctgga aatgatgctg ctatagccat
    15121 agctaaacat attggtaaga ctgaaaagaa ttttgtaaat atgatgaaca agaaagcaga
    15181 agaaatagga atgattgata cgtattattt taatccaaat gggcttccta tatatactga
    15241 cccagaacat aaagaacctc ctattgaaaa tatgtcaact gctcacgata tagttacact
    15301 tggaaagtat atgtatgacc attatgaaaa tcaagttaca agaataacca ctatgcaagt
    15361 atataatgat actaaaaagg attttacaca ttataacaca aatcccctac ttgtttcagt
    15421 acctggtgtt gatggaataa agacaggcta tacagataat gctggatatt gccttgcatt
    15481 ttctatgatg gtacctaaag atgctaaaaa cgaaagaaat catagattga taggtgttgt
    15541 acttggtgat ggtaacaaga aaaatagaat ttcttcttcc gctactttat tgaaatatgg
    15601 taaagataat ttccattcta aaaagatagc acataagggt gatattatag agactccttg
    15661 tgttgatggt attgatgatt ttaagataac agttaaggtt gataaagatt tgtatggtgt
    15721 tgtttcagat aacgaaaata tcaatcctaa ggtagtgttt aaaaatatga attatcctat
    15781 ccataaggga gatattgttg gtgttgcaaa gtattataat gactctggta aatttgttgg
    15841 aagtgttgat gtaaaaagtg aaagtaatat aggatgtatt cctttgaagg ataagattaa
    15901 gattaaggtt gctaagataa ataaaaaact tgagattaaa aattctgttt gttttaaagt
    15961 atagtaaagc atactattaa aagagtatat taaatttata aatactccac tttgtggagt
    16021 ttttttgttt tttacaaaaa attaaaatag tggcaacaat tgaggatgtg agggagagtt
    16081 gccactattt tgtatagggt attatattat gtttttaagc tagttttact atcttctatg
    16141 tttatataat aactcttgtg tgtgaattta attgataatt aatatgaaaa ttatgtgaag
    16201 ttttgatatt aggttatact ttgaacttta tctaacattt taaattttat atctctaaat
    16261 tatctaaaat tactatatca attaacttta catgataaaa aggctgtgta taaatttatt
    16321 tttaaaaatt ttactcacag ccttaaatta aattttctat ttaaagtata tataaatttt
    16381 attaaataat ttgcttttta tttcttattt cataaatcct agtattcttg ataacattaa
    16441 tgcaattgta gtaggtactt tccctgcttc aaatgtagct gttgctgtgt ttaatgctgt
    16501 tactgcatca tctatttctt tttgtgttgc atctgcttta tctaatattg cttgtgcttc
    16561 atctattgct gttttatatg t
//
LOCUS       DAMJVX010000078        15953 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.082, whole genome shotgun sequence.
ACCESSION   DAMJVX010000078 DAMJVX010000000
VERSION     DAMJVX010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15953
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.082"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            118..672
                     /gene="tcdR"
                     /locus_tag="PC407_002185"
     CDS             118..672
                     /gene="tcdR"
                     /locus_tag="PC407_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HDF2497629.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            1003..8103
                     /gene="tcdB"
                     /locus_tag="PC407_002186"
                     /gene_synonym="toxB"
     CDS             1003..8103
                     /gene="tcdB"
                     /locus_tag="PC407_002186"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HDF2497630.1"
                     /translation="MSLVNRKQLEKMANVRFRVQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTIVESATNDTLESFREN
                     LNDPRFDYNKFYRKRMEIIYDKQKNFINYYKTQREENPDLIIDDIVKIYLSNEYSKDI
                     DELNSYIEESLNKVTENSGNDVRNFEEFKGGESFKLYEQELVERWNLAAASDILRISA
                     LKEVGGVYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMVKLEAIMKYKEYIPGYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNAFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKKNYFEGSLGEDDNLDFSQNTVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGLDVDSLSTEIETAIDLA
                     KEDISPKSIEINLLGCNMFSYSVNVEETYPGKLLLRVKDKVSELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKIIVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLAECEINVISNIDTQVVEGRIEEAKSLTSDSINYIK
                     NEFKLIESISDALYDLKQQNELEESHFISFEDILETDEGFSIRFIDKETGESIFVETE
                     KAIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDATHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     VIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTAATTAIITSSLGIASG
                     FSILLVPLAGISAGIPSLVNNELILRDKATKVVDYFSHISLAESEGAFTSLDDKIMMP
                     QDDLVISEIDFNNNSITLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPVITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMNINIELNENDTWVIDVDNVVRDVTIESDKIKKGDLIENILSKLSIE
                     DNKIILDNHEINFSGTLNGGNGFVSLTFSILEGINAVIEVDLLSKSYKVLISGELKTL
                     MANSNSVQQKIDYIGLNSELQKNIPYSFMDDKGKENGFINCSTKEGLFVSELSDVVLI
                     SKVYMDNSKPLFGYCSNDLKDVKVITKDDVIILTGYYLKDDIKISLSFTIQDENTIKL
                     NGVYLDENGVAEILKFMNKKGSTNTSDSLMSFLESMNIKSIFINSLQSNTKLILDTNF
                     IISGTTSIGQFEFICDKDNNIQPYFIKFNTLETKYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVIISPNIYTDEINITPIYEANNTYPEVIVLDTNYIS
                     EKINININDLSIRYVWSNDGSDFILMSTDEENKVSQVKIRFTNVFKGNTISDKISFNF
                     SDKQDVSINKVISTFTPSYYVEGLLNYDLGLISLYNEKFYINNFGMMVSGLVYINDSL
                     YYFKPPIKNLITGFTTIGDDKYYFNPDNGGAASVGETIIDGKNYYFSQNGVLQTGVFS
                     TEDGFKYFAPADTLDENLEGEAIDFTGKLTIDENVYYFGDNYRAAIEWQTLDDEVYYF
                     STDTGRAFKGLNQIGDDKFYFNSDGIMQKGFVNINDKTFYFDDSGVMKSGYTEIDGKY
                     FYFAENGEMQIGVFNTADGFKYFAHHDEDLGNEEGEALSYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGISIINDGKYYFNDSGIMQIGFVTINNEVFYFS
                     DSGIVESGMQNIDDNYFYIDENGLVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFDPETKKAYKGINVIDDIKYYFDEN
                     GIMRTGLITFEDNHYYFNEDGIMQYGYLNIEDKTFYFSEDGIMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8225..8725
                     /gene="tcdE"
                     /locus_tag="PC407_002187"
     CDS             8225..8725
                     /gene="tcdE"
                     /locus_tag="PC407_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HDF2497631.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAVLLDAMTDEM
                     NAKDEK"
     gene            8804..8965
                     /locus_tag="PC407_002188"
     CDS             8804..8965
                     /locus_tag="PC407_002188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497632.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYMDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            9054..9236
                     /locus_tag="PC407_002189"
     CDS             9054..9236
                     /locus_tag="PC407_002189"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497633.1"
                     /translation="MRDEGQNDRENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            9453..>15953
                     /gene="tcdA"
                     /locus_tag="PC407_002190"
                     /gene_synonym="toxA"
     CDS             9453..>15953
                     /gene="tcdA"
                     /locus_tag="PC407_002190"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HDF2497634.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKNSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     LLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTIPRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKDSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKEYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPYISSHIPSLSV
                     YSAIGIKTENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENNGTKLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNTKIKLDKDTRNFIMPTITTDEIRNKLSYSFDGA
                     GGTYSLLLSSYPISMNINLSKDDLWIFNIDNEVREISIENGTIKKGNLIEDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNGSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLNNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIESNLVTGWQTINGKKYYFDINTGAASTSYKIINGKHFYFNNNGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSGSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTSIASTGYTIINGKYFYFNTDGIMQIGVFKVP"
BASE COUNT         6439 a         1698 c         2509 g         5307 t
ORIGIN      
        1 ttttcatata aaatttaatt tatttgccga ttatataatt ataatgactg atttaattcc
       61 aatgttgtca aaatttttaa ataaatcatc attataaata taagagagga tgattttatg
      121 caaaagtctt tttatgaatt aattgtttta gcaagaaata actcagtaga tgatttgcaa
      181 gaaattttat ttatgtttaa gccattagta aaaaaactta gtagagtttt acattatgaa
      241 gagggagaaa cagatttaat aatatttttt attgaattaa taaaaaatat taaattaagt
      301 agcttttcag aaaaaagcga tgctattata gtcaaatata ttcataaatc attactgaat
      361 aagacttttg agttgtctag aagatattct aaaatgaagt ttaattttgt agaatttgat
      421 gaaaatatct taaatatgaa aaataattat caaagtaagt ctgtttttga ggaagatatt
      481 tgttttttcg aatatatttt gaaagaatta tctggtattc aaagaaaagt tattttttat
      541 aaatatttaa aaggatattc tgatagagaa atatcagtga aattaaaaat atctagacaa
      601 gctgttaata aggctaaaaa tagagcattt aaaaaaataa aaaaagacta tgaaaattat
      661 tttaacttgt aattaatgaa tttaaagaaa tatttacaat agaaatcaaa ttttagaatt
      721 aactttattg taaaatcaat aacttaatct aagaatatct taatttttat attttatata
      781 gaacaaagtt tacatattta tttcagacaa cgtctttatt caatcgaaga gcaaattaat
      841 caactgagtg tcttcaattt aaaatgttag gaagtgaatg tatatgaaaa cctaagtaga
      901 tattagtata ttttataaat agaaaggagg atatataaaa gagttttagc atttagatgt
      961 aaaaatattc aataaaaata ttatagtaaa ggagaaaatt ttatgagttt agttaataga
     1021 aaacagttag aaaaaatggc aaatgtaaga tttcgtgttc aggaagatga atatgtagca
     1081 atattggatg ctttagaaga atatcataat atgtcagaga atactgtagt cgaaaaatat
     1141 ttaaaattaa aagatataaa tagtttaaca gatatttata tagatacata taaaaagtct
     1201 ggtagaaata aagccttaaa aaaatttaag gaatacctag ttacagaagt attagagcta
     1261 aagaataata atttaactcc agttgagaaa aatttacatt ttgtttggat tggaggtcaa
     1321 ataaatgaca ctgctattaa ttatataaat caatggaaag atgtaaatag tgattataat
     1381 gttaatgttt tttatgatag taatgcattt ttgataaaca cattgaaaaa aactatagta
     1441 gaatcagcaa caaatgatac acttgaatca tttagagaaa acttaaatga ccctagattt
     1501 gactataata aattttacag aaaacgtatg gagataatct atgataagca gaaaaatttc
     1561 ataaattact ataaaactca aagagaagaa aatcctgacc ttataattga tgatattgta
     1621 aagatatatc tttcaaacga gtattcaaag gatatagatg aacttaattc ctatattgaa
     1681 gagtcattaa ataaagttac agaaaatagt gggaatgatg ttagaaactt tgaagaattt
     1741 aaaggtggag agtcattcaa attatatgaa caagagttgg tagaaagatg gaatttggca
     1801 gctgcttctg acatattaag aatatctgcc ttaaaagaag ttggtggtgt atatttagat
     1861 gttgatatgt taccaggaat acaaccagac ctatttgagt ctatagagaa acctagttca
     1921 gtaacagtgg atttttggga aatggtaaaa ttagaagcta taatgaaata caaagaatac
     1981 ataccaggat atacttcaga gcattttgat atgttggatg aagaagttca aagtagtttt
     2041 gaatctgttc tagcttctaa gtcagataag tcagaaatat tctcatcact tggcgatatg
     2101 gaggcatcac cactagaagt taaaattgca tttaatagta aaggtattat aaatcaagga
     2161 ctaatttctg tgaaagactc atattgtagc aatttaatag taaaacaaat cgagaacaga
     2221 tataagatat tgaataatag tttaaatcca gctattagcg aggataacga tttcaatact
     2281 acaacgaatg cttttattga tagtataatg gctgaagcta atgcagataa tggtagattt
     2341 atgatggaac taggaaagta tttaagagtt ggtttcttcc cagatgttaa aactactatt
     2401 aacttaagtg gccctgaagc atatgcggca gcttatcaag atttattaat gtttaaagaa
     2461 ggtagtatga atatccattt gatagaagct gacttaagaa actttgaaat ctctaaaact
     2521 aatatttctc aatcaactga acaagaaatg gctagcttat ggtcatttga cgatgcaaga
     2581 gctaaagctc aatttgaaga atacaaaaaa aattactttg aaggttctct tggagaagat
     2641 gacaatcttg acttttctca aaatacagta gttgacaagg agtatctttt agaaaaaata
     2701 tcttcattag caagaagttc agagagagga tatatacact atattgttca gttacaagga
     2761 gataaaatta gttatgaagc agcatgtaac ttatttgcaa agactcctta tgatagtgta
     2821 ctgtttcaga aaaatataga agattcagaa attgcatatt attataatcc tggagatggt
     2881 gaaatacaag aaatagacaa gtataaaatt ccaagtataa tttctgatag acctaagatt
     2941 aaattaacat ttattggtca tggtaaagat gaatttaata ctgatatatt tgcaggtctt
     3001 gatgtagatt cattatccac agaaatagaa acagcaatag atttagctaa agaggatatt
     3061 tctcctaagt caatagaaat aaacttactg ggatgtaaca tgtttagcta ttctgtaaat
     3121 gtagaggaga cttatcctgg gaaattatta cttagagtta aagataaagt atcagaatta
     3181 atgccatcta taagtcaaga ctctattata gtaagtgcaa atcaatatga agttagaata
     3241 aatagtgaag gaagaagaga attattggat cattctggtg aatggataaa taaagaagaa
     3301 agtattataa aggatatttc atcaaaagaa tatatatcat ttaatcctaa agaaaataaa
     3361 attatagtaa aatctaaaaa tttacctgaa ttatctacat tattacaaga aattagaaac
     3421 aattctaatt caagtgatat tgaactagaa gaaaaagtaa tgttagcaga atgtgagata
     3481 aatgttattt caaatataga tacacaagtg gtagaaggaa ggattgaaga agctaaaagc
     3541 ttaacttctg actctattaa ttatataaag aatgaattta aactaataga atctatttct
     3601 gatgcactat acgatttaaa acaacagaat gaattagaag agtctcattt tatatctttt
     3661 gaggatatat tggagactga tgaaggcttt agtataagat ttattgataa agaaactgga
     3721 gaatctatat ttgtagaaac tgaaaaggca atattctctg aatatgctaa tcatataact
     3781 gaagagattt ctaagataaa aggtactata tttgatactg taaatggtaa gttagtaaaa
     3841 aaagtaaatt tagatgctac acatgaagtg aatactttaa atgctgcatt ttttatacaa
     3901 tcattaatag aatataatag ttctaaagaa tctcttagta atttaagtgt agcaatgaaa
     3961 gttcaagttt atgctcaatt atttagtact ggtttaaata ctattacaga tgcagccaaa
     4021 gttgttgaat tagtatcaac tgcattagat gaaactatag atttacttcc tacattatct
     4081 gaaggattac ctgtaattgc aactatcata gatggtgtaa gtttaggtgc agcaatcaaa
     4141 gagctaagtg aaacaagtga cccattatta agacaagaaa tagaagctaa gataggtata
     4201 atggcagtaa atttaacagc agctacaact gcaatcatta cttcatcttt aggaatagct
     4261 agtggattta gtatactttt agttccttta gcaggaattt cagcaggtat accaagttta
     4321 gtaaacaatg agcttatcct aagagataaa gcaacaaaag ttgtagatta ttttagtcat
     4381 atatcattag ctgagtctga aggagcattt acttcattag atgataaaat aatgatgcca
     4441 caagatgatt tagtaatatc tgagatagac tttaataaca attcaataac tttaggtaaa
     4501 tgtgaaatct ggagaatgga aggtggctca ggtcatactg taactgatga tatagatcac
     4561 ttcttttcag caccatcaat aacatataga gagccacact tatctatata tgacgtattg
     4621 gaagtacaaa aagaagaact tgatttgtca aaagatttaa tggtattacc taatgctcca
     4681 aatagagtat ttgcttggga aacaggatgg acaccaggtt taagaagctt agaaaatgat
     4741 ggcacaaaac tgttagaccg tataagagat aactatgaag gtgagtttta ttggagatat
     4801 tttgctttta tagctgatgc tttaataaca acattaaaac caagatatga agatactaat
     4861 ataagaataa atttagatag taatactaga agctttatag tcccagtgat aactacagaa
     4921 tatataagag agaaattatc atattctttt tatggttctg gaggaactta tgcattatct
     4981 ctttctcaat acaatatgaa tataaacata gaattaaatg aaaatgatac ttgggttata
     5041 gatgtcgaca atgtcgtaag agatgtcact atagaatctg ataaaattaa aaaaggagat
     5101 ttaatagaga atattttatc taaattaagt attgaagaca ataaaattat tttagataat
     5161 catgaaatta atttctctgg aacattaaat ggaggtaatg gatttgtatc tttaacattc
     5221 tcaatcttag aaggaataaa tgcagttata gaagttgatt tattatctaa atcatataaa
     5281 gttcttattt ctggtgaact aaaaacattg atggcaaatt caaattctgt tcaacagaaa
     5341 atagattata taggattgaa cagcgaatta caaaaaaata taccttatag ttttatggat
     5401 gataaaggaa aagaaaatgg atttattaat tgttctacaa aagaaggttt atttgtatct
     5461 gaattatctg atgtagttct tataagtaaa gtttatatgg acaatagtaa acctctattt
     5521 ggatattgta gtaatgattt gaaagatgtt aaagtcataa ctaaagatga cgttattata
     5581 ttaacaggat attatttaaa agatgatata aaaatctctc tttcttttac tatacaagat
     5641 gaaaatacta taaaattaaa tggagtatat ttagatgaaa atggagtagc tgaaatattg
     5701 aaatttatga ataaaaaagg tagtacaaat acttcagatt ctttaatgag ctttttagaa
     5761 agtatgaata taaaaagtat tttcataaat tccttacaat ctaatactaa gcttatatta
     5821 gatactaatt ttataataag tggtactact tctattggtc aatttgagtt tatttgtgat
     5881 aaagataata atatacaacc atatttcatt aagtttaata cactagaaac taaatatact
     5941 ctatatgtag gtaatagaca aaatatgata gtagaaccaa attatgattt agatgattct
     6001 ggagatatat cttcaactgt cattaatttt tctcagaaat acctttatgg aatagacagt
     6061 tgtgttaata aagttataat ttcgccaaat atatatacag atgaaataaa cataacacct
     6121 atatatgaag caaataatac ttatccagaa gtgattgtat tagatacaaa ttatataagt
     6181 gaaaaaatca atattaatat caatgattta tctatacgat atgtatggag taatgatgga
     6241 agtgatttta ttcttatgtc aactgatgaa gagaacaagg tatcacaagt taaaataaga
     6301 tttactaatg tttttaaagg taatactata tcagataaga tatcttttaa ttttagtgat
     6361 aagcaagatg tatctataaa taaagttatt tcaacattta caccttcata ttatgtggaa
     6421 ggattactta attatgattt aggtctgatt tctttataca atgagaaatt ttatattaat
     6481 aactttggaa tgatggtgtc tggattagta tatattaatg attcattata ttatttcaag
     6541 ccaccaataa agaacttgat aactggattt acaactatag gtgatgataa atactacttt
     6601 aatccagata atggtggagc tgcttcagtc ggagaaacaa taattgatgg caaaaactac
     6661 tacttcagcc aaaatggagt gttacaaaca ggtgtattta gtacagaaga tggatttaaa
     6721 tattttgctc cagcagatac acttgatgaa aatctagagg gggaagcaat tgattttact
     6781 ggcaaactaa ctattgatga aaatgtttat tattttggag ataattatag agcagctata
     6841 gaatggcaaa cattagatga tgaagtgtac tattttagta cagatacagg tagagctttt
     6901 aaagggctaa atcaaatagg tgatgataaa ttctatttca actctgatgg tattatgcaa
     6961 aaaggatttg ttaatataaa tgataagaca ttctattttg atgattctgg tgtgatgaag
     7021 tcaggatata ctgaaataga tggaaaatat ttttactttg ctgagaatgg agaaatgcaa
     7081 ataggagtat ttaatacagc agatggattt aaatattttg ctcatcatga tgaagattta
     7141 ggaaatgaag aaggtgaagc actttcatat tctggtatac ttaattttaa caataagatt
     7201 tattattttg atgattcatt tacagcagta gttggatgga aggatttaga agatggttca
     7261 aaatattact ttgatgaaga tacagcagaa gcatatatag gtatctcaat aattaatgat
     7321 ggtaaatatt attttaatga ttctggaatc atgcaaattg gatttgtcac aataaataat
     7381 gaagtatttt atttctctga ttctggaata gtagaatctg gaatgcaaaa tatagatgat
     7441 aattatttct atatagacga aaatggtcta gttcaaattg gtgtatttga cacttcagat
     7501 ggatataaat actttgcacc agctaatact gtaaatgata atatctatgg acaagcagtt
     7561 gaatatagtg gtttagttag agttggtgaa gatgtatatt attttggaga aacatataca
     7621 attgagactg gttggatata tgatatggaa aatgaaagtg ataaatatta tttcgatcca
     7681 gaaactaaaa aagcatataa aggtattaat gtaattgatg atataaaata ctattttgat
     7741 gagaatggaa taatgagaac aggtcttata acatttgaag ataatcatta ctattttaat
     7801 gaagatggta ttatgcaata tggttatcta aatatagaag ataagacgtt ctactttagt
     7861 gaagatggta ttatgcagat tggagtattt aatacaccag atggatttaa atattttgca
     7921 catcaaaata ctttagatga gaattttgag ggagaatcaa taaactatac tggttggtta
     7981 gatttagatg aaaagagata ttattttaca gatgaatata ttgcagcaac tggttcagtt
     8041 attattgatg gtgaggagta ttattttgat cctgatacag ctcaattagt gattagtgaa
     8101 tagataaaaa tacgttaaat atatcctctt atacttaaat atataaaaat aaacaaaatg
     8161 atacactaca taaagtgttc tatctaatat gaagatttac caataaaaag gtgaactatg
     8221 atgaatgcac agtagttcac ctttttatat ttctaatggt aacaaaatat ttttttatat
     8281 aaacctagga ggcgttatga atatgacaat atctttttta tcagagcata tatttataaa
     8341 gttagtaatt ttaactatat catttgatac attattagga tgtttaagtg caataaaaag
     8401 tcgtaaattt aattctagtt ttggaataga tggaggaatc agaaaagtag caatgatagc
     8461 atgtatattt tttttatcag tagttgacat tcttacaaag tttaactttt tatttatgtt
     8521 accacaagat tgtatcaatt ttttaagact aaaacatctt ggaatatctg aatttttctc
     8581 tattttattt attttatatg aaagtgtaag catattaaaa aatatgtgct tatgtggatt
     8641 accagtacct aagagattaa aggaaaaaat agcagtttta ctagatgcaa tgacagatga
     8701 aatgaatgct aaggatgaaa agtaagtaat ggtagatata ataaaaatat taacaaataa
     8761 aaagtgttat ccaaataaga atagctgaaa gttatcataa ttcatgaaac tgataatgaa
     8821 aacgagggag cagatgccaa gagacacaca agtattaaat acatataatt tcgaagcaag
     8881 tgttcattac tatatggatg acaaggtagt atatcaaaca ttggttcaca aagatggtgc
     8941 atggtcagtt ggtaaaatct attaagctac attagttaca gatatcacaa actataatag
     9001 ttaaacatag aaatatgtgt aaattgtgat ggaaattatt caaaaacaca aaaatacgtg
     9061 atgaaggaca aaatgataga gaaaataagt atcaaacctt aataaatgat ttaattgata
     9121 gtttaaaagt tataggaaaa atatataaag aaataaaaac attaaaaaaa tataagatat
     9181 gtttacaaat tactatcaga caatctcctt atctaataga agagtcaatt aactaattga
     9241 gtatctttaa attgaaatgt taggaagtga tttaaatatg aaaacttaaa ttataaaaaa
     9301 tcaatattaa tttatcttta aaaaatagaa aggagtgtat aagatttatt ttcaaagttt
     9361 aaaaacaaga aaatcaattt aaatttcaga aggaataaat gtggttatag aagtggattt
     9421 attatcaaaa ataatagtac taggaggttt ttatgtcttt aatatctaaa gaagagttaa
     9481 taaaactcgc atatagcatt agaccaagag aaaatgagta taaaactata ctaactaatt
     9541 tagacgaata taataagtta actacaaaca ataatgaaaa taaatattta caattaaaaa
     9601 aactaaatga atcaattgat gtttttatga ataaatataa aaattcaagc agaaatagag
     9661 cactctctaa tctaaaaaaa gatatattaa aagaagtaat tcttattaaa aattccaata
     9721 caagtcctgt agaaaaaaat ttacattttg tatggatagg tggagaagtc agtgatattg
     9781 ctcttgaata cataaaacaa tgggctgata ttaatgcaga atataatatt aaactgtggt
     9841 atgatagtga agcattctta gtcaatacac taaaaaaggc tatagttgaa tcttctacca
     9901 ctgaagcatt acagctacta gaggaagaga ttcaaaatcc tcaatttgat aatatgaaat
     9961 tttacaaaaa aaggatggaa tttatatatg atagacaaaa aaggtttata aattattata
    10021 aatctcaaat caataaacct acagtaccta caatagatga tattataaag tctcatctag
    10081 tatctgaata taatagagat gaaactttat tagaatcata tagaacaaat tctttgagaa
    10141 aaataaatag taatcatggg atagatatca gggctaatag tttgtttaca gaacaagagt
    10201 tattaaatat ttatagtcag gagttgttaa atcgtgggaa tttagctgca gcatctgaca
    10261 tagtaagatt attagcccta aaaaattttg gcggagtata tttagatgtt gatatgcttc
    10321 caggtattca ctctgattta tttaaaacaa tacctagacc tagctctatt ggactagacc
    10381 gttgggaaat gataaaatta gaggctatta tgaagtataa aaaatatata aataattata
    10441 catcagaaaa ctttgataaa cttgatcaac aattaaaaga taattttaaa ctcattatag
    10501 aaagtaaaag tgaaaaatct gagatatttt ctaaattaga aaatttaaat gtatctgatc
    10561 ttgaaattaa aatagctttc gctttaggca gtgttataaa tcaagccttg atatcaaaac
    10621 aaggttcata tcttactaac ctagtaatag aacaagtaaa aaatagatat caatttttaa
    10681 accaacacct taacccagcc atagagtctg acaataactt cacagatact actaagattt
    10741 ttcatgattc actatttaat tcagctaccg cagaaaactc tatgttttta acaaaaatag
    10801 caccatactt acaagtaggt tttatgccag aagctcgctc cacaataagt ttaagtggtc
    10861 caggagctta tgcatcagct tactatgatt tcataaattt acaagaaaat actatagaaa
    10921 aaactttaaa agcatcagat ttaatagaat ttaaattccc agaaaataat ctatctcaat
    10981 tgacagaaca agaaataaat agtctatgga gctttgatca agcaagtgca aaatatcaat
    11041 ttgagaaata tgtaagagat tatactggtg gatctctttc tgaagacaat ggggtagact
    11101 ttaataaaaa tactgccctc gacaaaaact atttattaaa taataaaatt ccatcaaaca
    11161 atgtagaaga agctggaagt aaaaattatg ttcattatat catacagtta caaggagatg
    11221 atataagtta tgaagcaaca tgcaatttat tttctaaaaa tcctaaaaat agtattatta
    11281 tacaacgaaa tatgaatgaa agtgcaaaaa gttacttttt aagtgatgat ggagaatcta
    11341 ttttagaatt aaataaatat aggatacctg aaagattaaa aaataaggaa aaagtaaaag
    11401 taacctttat tggacatggt aaagatgaat tcaacacaag cgaatttgct agattaagtg
    11461 tagattcact ttccaatgag ataagttcat ttttagatac cataaaatta gatatatcac
    11521 ctaaaaatgt agaagtaaac ttgcttggat gtaatatgtt tagttatgat tttaatgttg
    11581 aagaaactta tcctggtaag ttactattaa gtattatgga caaaattact tccactttac
    11641 ctgatgtaaa taaagattct attactatag gagcaaatca atatgaagta agaattaata
    11701 gtgagggaag aaaagaactt ctagctcact caggtaaatg gataaataaa gaggaagcta
    11761 ttatgagcga tttatctagt aaagaataca ttttttttga ttccatagat aataagctaa
    11821 aagcaaagtc caagaatatt ccaggtttag cgtcaatatc agaagatata aaaacattat
    11881 tacttgatgc aagtgttagt cctgatacaa aatttatttt aaataatctt aagcttaata
    11941 ttgaatcttc tattggtgat tacatttatt atgaaaaatt agaacctgtt aaaaatataa
    12001 tccacaattc tatagatgat ttaatagatg agttcaatct acttgaaaat gtatctgatg
    12061 aattatatga attaaaaaaa ttaaataatc tagatgagaa gtatttaata tcttttgaag
    12121 atatctcaaa aaataattca acttattctg taagatttat taacaaaagt aatggtgaat
    12181 cagtttatgt agagacagaa aaagaaattt tttcaaaata tagcgaacat attacaaaag
    12241 aaataagtac tataaagaat agtataatta cagatgttaa tggtaattta ttggataata
    12301 tacagttaga tcatacttct caagttaata cattaaacgc agcattcttt attcaatcat
    12361 taatagatta tagtagcaat aaagatgtac tgaatgattt aagtacctca gttaaggttc
    12421 aactttatgc tcaactattt agtacaggtt taaatactat atatgactct atccaattag
    12481 taaatttaat atcaaatgca gtaaatgata ctataaatgt actacctaca ataacagagg
    12541 ggatacctat tgtatctact atattagacg gaataaactt aggtgcagca attaaggaat
    12601 tactagacga acatgaccca ttactaaaaa aagaactaga agctaaggtg ggtgttttag
    12661 caataaatat gtcattatct atagctgcaa cggtagcttc aattgttgga ataggtgctg
    12721 aagttactat tttcttatta cctatagctg gtatatctgc gggaatacct tcattagtta
    12781 ataatgaatt aatattgcat gataaggcaa cttcagtggt aaactatttt aatcatttgt
    12841 ctgaatctaa agaatatggc cctcttaaaa cagaagatga taaaatttta gttcctattg
    12901 atgatttagt aatatcagaa atagatttta ataataattc gataaaacta ggaacatgta
    12961 atatattagc aatggagggg ggatcaggac acacagtgac tggtaatata gatcactttt
    13021 tctcatctcc atatataagc tctcatattc cttcattatc agtttattct gcaataggta
    13081 taaaaacaga aaatctagat ttttcaaaaa aaataatgat gttaccaaat gctccttcaa
    13141 gagtgttttg gtgggaaact ggagcagttc caggtttaag atcattggaa aataatggga
    13201 ctaaattgct tgattcaata agagatttat acccaggcaa attttactgg agattctatg
    13261 cctttttcga ttatgcaata actacattaa aaccagtgta tgaagacact aatactaaaa
    13321 ttaaactaga taaagatact agaaacttta taatgccaac tataactact gacgaaatta
    13381 gaaacaaatt atcttattca tttgatggag caggaggaac ttactcttta ttattatctt
    13441 catatccaat atcaatgaat ataaatttat ctaaagatga tttatggata tttaatattg
    13501 ataatgaagt aagagaaata tctatagaaa atggtactat taaaaaagga aatttaatag
    13561 aagatgtttt aagtaaaatt gatataaata aaaataaact tattataggc aatcaaacaa
    13621 tagatttttc aggtgatata gataacaaag atagatatat attcttgact tgtgagttag
    13681 atgataaaat tagtttaata atagaaataa atcttgttgc aaaatcttat agtttgttat
    13741 tgtctgggga taaaaattat ttgatatcca atttatctaa tactattgag aaaatcaata
    13801 ctttaggcct agatagtaaa aatatagctt acaattacac tgatgaatct aataataaat
    13861 attttggagc tatatctaaa acaagtcaaa aaagcataat acattataaa aaagacagta
    13921 aaaatatatt agaattttat aatggcagta cattagaatt taacagtaaa gactttattg
    13981 ctgaagatat aaatgtattt atgaaagatg atattaatac tataacagga aaatactatg
    14041 ttgataataa tactgataaa agtatagatt tctctatttc tttagttagt aaaaatcaag
    14101 taaaagtaaa tggattatat ttaaatgaat ccgtatactc atcttacctt gattttgtga
    14161 aaaattcaga tggacaccat aatacttcta attttatgaa tttatttttg aacaatataa
    14221 gtttctggaa attgtttggg tttgaaaata taaattttgt aatcgataaa tactttaccc
    14281 ttgttggtaa aactaatctt ggatatgtag aatttatttg tgacaataat aaaaatatag
    14341 atatatattt tggtgaatgg aaaacatcgt catctaaaag cactatattt agcggaaatg
    14401 gtagaaatgt tgtagtagag cctatatata atcctgatac gggtgaagat atatctactt
    14461 cactagattt ttcctatgaa cctctctatg gaatagatag atatatcaat aaagtattga
    14521 tagcacctga tttatataca agtttaataa atattaatac caattattat tcaaatgagt
    14581 actaccctga gattatagtt cttaacccaa atacattcca caaaaaagta aatataaatt
    14641 tagatagttc ttcttttgag tataaatggt ctacagaagg aagtgacttt attttagtta
    14701 gatacttaga agaaagtaat aaaaaaatat tacaaaaaat aagaatcaaa ggtatcttat
    14761 ctaatactca atcatttaat aaaatgagta tagattttaa agatattaaa aaactatcat
    14821 taggatatat aatgagtaat tttaaatcat ttaattctga aaatgaatta gatagagatc
    14881 atttaggatt taaaataata gataataaaa cttattacta tgatgaagat agtaaattag
    14941 ttaaaggatt aatcaatata aataattcat tattctattt tgatcctata gaatctaact
    15001 tagtaactgg atggcaaact atcaatggta aaaaatatta ttttgatata aatactggag
    15061 cagcttcaac tagttataaa attattaatg gtaaacactt ttattttaat aataatggtg
    15121 tgatgcagtt aggagtattt aaaggacctg atggatttga gtattttgca cctgccaata
    15181 ctcagaataa taacatagaa ggtcaggcta tagtttatca aagtaaattc ttaactttga
    15241 atggcaaaaa atattatttt gataatgact caaaagcagt cactggatgg aggattatta
    15301 acaatgagaa atattacttt aatcctaata atgctattgc tgcagtcgga ttgcaagtaa
    15361 ttgacaataa taagtattat ttcaatcctg acactgctat catctcaaaa ggttggcaga
    15421 ctgttaatgg tagtagatac tactttgata ctgataccgc tattgccttt aatggttata
    15481 aaactattga tggtaaacac ttttattttg atagtgattg tgtagtgaaa ataggtgtgt
    15541 ttagtggctc taatggattt gaatatttcg cacctgctaa tacttataat aataacatag
    15601 aaggtcaggc tatagtttat caaagtaaat tcttaacttt gaatggtaaa aaatattact
    15661 ttgataataa ctcaaaagca gttaccggat ggcaaactat tgatagtaaa aaatattact
    15721 ttaatactaa cactgctgaa gcagctactg gatggcaaac tattgatggt aaaaagtatt
    15781 actttaatac taacactgct gaagcagcta ctggatggca aactattgat ggtaaaaaat
    15841 attactttaa tactaacact tctatagctt caactggtta tacaattatt aatggtaaat
    15901 atttttattt taatactgat ggtattatgc agataggagt gtttaaagta cct
//
LOCUS       DAMJVX010000079        15836 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.083, whole genome shotgun sequence.
ACCESSION   DAMJVX010000079 DAMJVX010000000
VERSION     DAMJVX010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15836
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.083"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            232..900
                     /locus_tag="PC407_002191"
     CDS             232..900
                     /locus_tag="PC407_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497635.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLASNFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            902..1387
                     /locus_tag="PC407_002192"
     CDS             902..1387
                     /locus_tag="PC407_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF2497636.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            1448..2569
                     /locus_tag="PC407_002193"
     CDS             1448..2569
                     /locus_tag="PC407_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDF2497637.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMQFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            2850..4040
                     /locus_tag="PC407_002194"
     CDS             2850..4040
                     /locus_tag="PC407_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2497638.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDIVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLVRKGFKH
                     TPLQSLCPEYKDKIITCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    4174..4429
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4488..5489
                     /locus_tag="PC407_002195"
     CDS             4488..5489
                     /locus_tag="PC407_002195"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDF2497639.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            5593..5982
                     /locus_tag="PC407_002196"
     CDS             5593..5982
                     /locus_tag="PC407_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDF2497640.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIIKKDKHSNIIENVEFSIKDEEE"
     gene            6143..7351
                     /locus_tag="PC407_002197"
     CDS             6143..7351
                     /locus_tag="PC407_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF2497641.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(7438..8514)
                     /locus_tag="PC407_002198"
     CDS             complement(7438..8514)
                     /locus_tag="PC407_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2497642.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            8743..9270
                     /gene="hpt"
                     /locus_tag="PC407_002199"
     CDS             8743..9270
                     /gene="hpt"
                     /locus_tag="PC407_002199"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2497643.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            9359..10291
                     /locus_tag="PC407_002200"
     CDS             9359..10291
                     /locus_tag="PC407_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDF2497644.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIVGWMLGIFFTVIF
                     IKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            10353..11579
                     /locus_tag="PC407_002201"
     CDS             10353..11579
                     /locus_tag="PC407_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2497645.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTRFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINANTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            11663..11884
                     /locus_tag="PC407_002202"
     CDS             11663..11884
                     /locus_tag="PC407_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2497646.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            12073..12243
                     /locus_tag="PC407_002203"
     CDS             12073..12243
                     /locus_tag="PC407_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497647.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(12336..12797)
                     /locus_tag="PC407_002204"
     CDS             complement(12336..12797)
                     /locus_tag="PC407_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497648.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQHFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            12980..13447
                     /locus_tag="PC407_002205"
     CDS             12980..13447
                     /locus_tag="PC407_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497649.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMIAFNLNMILGIGVFLIGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            13490..15253
                     /locus_tag="PC407_002206"
     CDS             13490..15253
                     /locus_tag="PC407_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HDF2497650.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDTIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMKDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            15261..>15836
                     /locus_tag="PC407_002207"
     CDS             15261..>15836
                     /locus_tag="PC407_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2497651.1"
                     /translation="MDSNDKKGLKSTEIIIINGSPRINKNCSFIANKVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRNKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIY"
BASE COUNT         6195 a         1607 c         2781 g         5253 t
ORIGIN      
        1 gcaagataag gaaaatagct ttttatattg taatgtatat tttaaatagt taataaaaaa
       61 atattatatt tttaaacttg aaaagtaagt aattataagc tatttaatcg ctggaataaa
      121 tttacaataa aaaaataaat acaaaggctt tatcattaag gtattggcat aagttttgct
      181 tattaaacaa gtatactaat aaaattcatt taaacaggag ggagaattta tatgagtaaa
      241 ttagaatcaa caacagaaaa agttgaggta aaatggtatg ggtatgtagc attaatattg
      301 ggagcattat ttttttcagg aatatttaaa gatgcaccag gaccattaaa agtattggat
      361 tttaacaatg tattaggttc ttttggaaca ttgggaacaa ttaatgatgg tgtaggtaca
      421 ttagcttcaa actttagagg tgatggaggt actggccctc gagatggatg gctatatgca
      481 ttgacattaa taccatcagt tatgtttgca cttggagtag ttagagttat agaccactta
      541 gatggaatga aggcagctca aaagcttctt agtccactat taaagccatt attagggtta
      601 cctggatttg caggactaac tttgatagct agtttacagt ctacagatgc agctgcatct
      661 atgactaaag aacttaaaga tgatgggtat atagatgaaa aacaaaaagc tgtattttgt
      721 gcatttcaat tttcaggtgc gtctgcaatt acgaatttct ttgcttctgg agcagcacta
      781 tttccattta taggagatgt accaatattt ataccacttg ctttaatatt aattatgaag
      841 tttgttgggg cgaacttact tagattatat ttaaataaat ttgacaaggg ggaagcttaa
      901 catgagtgag gtagcaaaaa aagaagtaaa agttaaaaaa ggaaatgtga tagatgcgtt
      961 tattggagga gctagaaatg gattccaaat aagtacaaat tcaatggctc caaatgtcat
     1021 cttcggtttt gctataattt ctgtgttcaa tttaacaggt cttttggatg taatagggac
     1081 aatatttaca cctgtaatga gtatctttgg acttccaggt gttgcagcaa cagctataat
     1141 gacaatattt atatcaatgg gaggagcatc tggagtaatc gcaggtttat ttacagcagg
     1201 tcaaataagt caagcacatg ttgcaattct attgccaatg ttattgcttt caggagcgca
     1261 gatacaattt atggggagac ttttgggaac agctgagtta aaaactaaat attatccaca
     1321 tatgtttgtt atagcaactt taaatggttg tattgcaatg cttataatga attatttcgt
     1381 agtataggta attataataa aaatttaata taaatggaaa gtttaaattt taaggaggag
     1441 ctagattatg ggaactaaca aagattatat tgagtatgta cataaagtgt atgattatct
     1501 acacactata ccagaaaaag gatttgaaga atataagaca tcagagtttg ttttaaaaga
     1561 gcttgaaaaa atggggtttg aaaatattaa taaaggagtt gcaggaactg gtattatagt
     1621 aactatagat tctaaaaaag aaggaccagt atttgctata agagcagata tggatgcatt
     1681 ggaatttatc atagatggag aaaagaaaat gatacatgct tgtggtcatg atggtcatac
     1741 ttctatgctt ttaacagctg caaagttaat aaaagagaga aatttagtaa aaagaggtaa
     1801 gttaaaaata ctttttcaac caggtgaaga aaaattatat ggagcattaa gagtaattga
     1861 ttctggtttg ttggatgatg tagaagaaat ggtagggatg catttaagac ctggacaaga
     1921 ttgtaggttg ggaaaggcga tacctgcaat gtaccattgt gctagctgta taatgcaatt
     1981 tacgattaaa ggtatcactt gtcatggttc taaaccacat ttaggagtaa atgcagtaga
     2041 tgctgtctgt ttagcagtga atgcaataaa tgcaataaga gtaaatccag ctataccaaa
     2101 ttctataaaa gtaacaaata tatcagttaa gggagaaact tataataata taccagatga
     2161 agcatttatg gctttagata taagggcaga aagtaatgat ataatagaag aattaaaaga
     2221 aaaagcaata catgctgtaa aaactgctgt aagttctata aatgcagaag ctgtactaat
     2281 ttccaaagat ggagtaccag cagcagaatt tgatgaagaa cttgtaaact tggctgaaga
     2341 aactataaaa gatgtattgg gagctgatgc atctataggt atatttaaaa attctggtgg
     2401 agaagatttc cattattata cacaaaaact aaaatgtaaa actacatata ttggattagg
     2461 agctgatgct acaccaggat ttcataatcc aaatgtaaca tttgatacga aagctttaga
     2521 atatggtgta gatatatggt gtagattggt tgaaaagaga ttgggataat atccaaacta
     2581 ataattctac atttaaaatt ataattatag aagttataga aaaagattgc ttatgttaat
     2641 tagaaaataa ttaatatgag caatcttatt ttgatataat gaaactgatt ttggaatatt
     2701 gaaaaaatat gatggaaaag taattatatt ttaaatctat tggacaaaaa agtgatatta
     2761 ttataatatt aagaattgta aaataatacc aaagtgcatc tagtaatgga cttaacaatt
     2821 ataaaaattt tattcttagg agggaaaaaa tgagtaagta taattttgac agagtaatag
     2881 atagagtagg aacagattgt gtaaaatggg attttagaac taattgttca acaaaagcac
     2941 aaaaagatgg tttgcctttt tggattgctg atatggattt tgaatgtgca gaaccaataa
     3001 tagaagcatt acgtaaaaaa gttgaacata aaatatttgg atatagttca aatgatagtg
     3061 ataaatactt taatgcagtt tgtgattggt ataaaaggag atttaactgg gaaataaata
     3121 gaaaagatat tatttttagt cctggtgttg tacctgctgt tgctatttta gtaagaatat
     3181 taactaattc taatgaaggt gtaatagtcc aaaaaccagt gtattaccca tttgaagcta
     3241 agataaagag caataatagg gacattgtaa acaatcctct aatatatgag aatggtactt
     3301 ataggatgga ttatgatgat ttagaagaaa aagctaagtg tagcaacaat aaagtactga
     3361 tactttgtag tcctcacaac cctgttggaa gagtttggag agaagatgaa ttaaagaagg
     3421 ttgtagaaat atgtaaaaaa tatgacctat ggataatagc tgatgagata cattctgatt
     3481 tagttagaaa aggatttaaa catactcctt tacagtcatt atgcccagag tacaaggata
     3541 aaataattac atgtatagct ccaagtaaaa catttaatct ggcaggaatg cagttgtcta
     3601 atataatagt gaataatgat gaactaaaaa agaaatatca agaagaggtt acagctgttg
     3661 gagttgcaac ttcaccaaat ccctttgcta ttgtagctac aatagcagca tataatgaaa
     3721 gtgaagactg gcttaatgaa ttaaatgatt acttagataa taatatacaa tttattgatg
     3781 aatacttaaa agaaaaccta ccaaaagtaa aacttgttta tccagaagga acatatctag
     3841 catggttaga ttttactgct tatggattaa atgaagtaga acttgaggat ttaatgttta
     3901 aaaaagctaa tgtattattt gatgaaggtt atatatttgg aaaagaagga ataggatttg
     3961 aaagaataaa tgtagcttgt ccacagtctt tattaaaaga atgtatggac agattaaaga
     4021 ctatatttga aaatttataa agtataggta aaaatttata aagtgtttaa aaataaaaaa
     4081 ataataaact tgacaaattt taactaatca cttatcatac tattatgaat aaatagaaaa
     4141 cataagagag ctaaatttta ttgaattaaa ctcttaaaga ctttgaaagg aagagtagtt
     4201 atagaatgta attaaagcga gttggtgatg gtgtgagacc aatatgaagt ctataatgaa
     4261 gaacatcctg gagttgctaa ccgaaatttt aaaagagtag gcttagtcgg aaccaaaccg
     4321 ttatcatatt ggggtgtatc gagtaatatc tgtacattgt gagctgggtt tgaatttatt
     4381 tcaaactaat cagggtggta ccgcggaaaa gcaacctttc gtccctttta tatagggatg
     4441 aagggttttt ttaatggaaa aatatataaa tatagggagt gaatgatatg agcacaataa
     4501 taccaaagga ttataaaagt agtttgaacg taattgatac acaaatagct ataaaaaaat
     4561 taaaagattt ttttgaaatg agactaagta atgaattaaa tcttataaga gtttcttctc
     4621 cgttatttgt attgccagaa acaggtgcaa atgataattt aaatggagaa aaggctgtaa
     4681 gctttgatat accatttatg aataaaaatg cagaaattgt tcaatcactt gcaaaatgga
     4741 aaagattagc ccttaaaaaa tatgggtttg aagttggtag tggtttatat acagatatga
     4801 atgcaataag aaaaaatgaa gaattagaca atatacattc tctttatgta gaccaatggg
     4861 attgggaatt ggttatagat aaaagtagta gaaatgaaaa gactctaaaa gatgttgtaa
     4921 aaagattgta tggagtattt aaagatactg aaatttttgt atgtagtatg tatgaaggta
     4981 taaaagaaat attaccagaa gaaataactt ttataacatc acaagagtta gaagatatgt
     5041 atccagaatt atcaccgaag gaaagagaag ataaaattgt aaaagagaag aaagcagtat
     5101 ttataactca aataggaaaa acattaatat caggagaaaa acatgatgga agaagccctg
     5161 attatgatga ttgggaattg aatggggata tattatttta caatcctgtt ttagattcag
     5221 cattagaatt atcttcaatg ggaattagag ttgatgaaga atctcttgat agacaattga
     5281 aacttgctgg atgtgaagag agaaaagagt ttgactatca taagatgctt ttaaatggcg
     5341 aacttccgta tacaattggt ggaggaattg gtcagtcaag aatatgtatg tatttcttac
     5401 aaaaagctca tataggagaa gtacaagttg gagtatggcc acaggatatg attcaaaatt
     5461 gtagtagtgc aggaattgaa ttattataat ttaaatataa actaattgat aaaatttata
     5521 tctaatatgt tcatcaaatt aattatatcg gttataatgt agaataacct ataattataa
     5581 gaggtgataa atatggagtt tttaaaaata atcattaata tagttttaga tattttaaaa
     5641 aaaattttgg tcagatttaa aaatgctaaa tttggtttgg tttttgtatt tgatttactt
     5701 aaactacctg attttatgac agataaaaga atcaatatag ttgataaaat aaaggtaatt
     5761 tctgtattaa tttttactat atcctacttt gtatctgggg ttgatataat tccagagatg
     5821 attgcagggg catttggttt tatagatgat gctatagttt taatttggag tataggtata
     5881 gtgaatgaag aaattaataa gtatagagtt attataaaaa aagataaaca ttcaaatata
     5941 attgaaaatg tagaattttc tataaaagat gaagaagaat aacaatttat atatgttatt
     6001 ctttttttta ttaacaaaaa atggttaaat tgttgacgga gaggtaaaac tctattatca
     6061 taataagtgg atatccaata tttaactaag tattcaatgt gcaagaatgc ttaatttaaa
     6121 aaagttatgg agggtaaata ttatgaagaa aaaaataggt ttagtgccca aactaatcat
     6181 cggtatcata gtcggtattt taatcggaag ttatgcacca gaaataatag tacaaatatt
     6241 agtaacagtt agtaccttat tctcagcatt cctaaaattt gttataccat ttataattat
     6301 aggttttgta acagcaggta tagctgattt agcaacaggt gcaggaaagt tattggggat
     6361 aactacggga atagcatatg gttctacctt aatagcagga ttattatcat ttgcagtatc
     6421 tacattaatc ttcccaaatt ttattgatgc aagtgttgca agtcaaattg gagacccaga
     6481 ggcaggaatg ttagctccaa tattcacaat tccattagag cctatggtag atgtaacagc
     6541 tgcaatcgta tttgcatttg taatgggact tggaatatca gctttaagaa atcatggaaa
     6601 aggagagact ttatttaatt tcttccaaga atttcaagga atcgtaacga aaacgttgtc
     6661 aacagtgatt atacctttat tacctttata catagcagga acttttgcaa atataactat
     6721 agcaggggaa gtatggacaa tattaggggt attctggaaa gtatacttag tagtaatacc
     6781 tttacatttt gtatatatgg catgtcaatt cacagcagca ggtgtatttt caggaaaaaa
     6841 tccagtaaga atgttaaaaa atcaagtacc tggatattta acagctatag gaactcaatc
     6901 ttcagcagca actataccag ttaatgttgg ttgtgcagaa aaaaatggag taagtaagca
     6961 aataagagaa tttgttgtac ctctttgtgc aacaatacat ttatcaggta gtatgatatc
     7021 aataacagga tttacaactg cagttttaat gatgaatggt atgccacatg gaataggtgt
     7081 attattccct tatattgcaa tgttaggtat agcaatggtt gcagcaccag gagcaccagg
     7141 tggggctata atgagtgcac ttcctttcct accaatggtt ggaataccat cagatggtgg
     7201 gcttgcaagt ttaatgatag ctttatactt aactcaagac agttttggta cagcatgtaa
     7261 tgtatcagga gataatgcaa tagcagtagt agtagataaa ataaatgatg gaatgagtaa
     7321 aaaaggaaac aaagaagagc aaagtgctta gttttttaat aatcaaaaat gagttatctc
     7381 aaatagagat agctcatttt ttatggtata aaaaaattat aaaataaagg gattttttta
     7441 tttaaaaatt gctttaagag atgatattaa taatttattt tcattaggtt ttaatataca
     7501 tactctaaag aaatactcat ctagaccttc aaatgaacaa caatctctta ttattatttt
     7561 ttgtggaagc agttgttctc gaagcgattt agcagttaat tcctttgttt tgattttaca
     7621 caatatgaaa ttacctttag tattgtatat atctaagctt tttatattct taagctcttt
     7681 aagcaggtaa tctctttcag tgttcattaa ggaaatagta tttgatataa aatctaaatc
     7741 actaaacatg atttccccca ttttagaagc tattatgttt atattccata aatctagatt
     7801 tttatttatt tcatttttga cattagtgtt agatataaga ccatatccaa gccttattcc
     7861 aggcgttgaa aaaaattttg atgtacctct tataacgaat aaattagaat aatcatcaac
     7921 taattgagta caagagtatg tatctgtatc agtaaactct acataagtct catcaatcat
     7981 taaaaatgag tctgtatttt ttaaaatttc tctcacttct actttagtaa atgcaaatcc
     8041 agttggatta ctaggattac agattactac caaatcataa tctttagcat tgatagtttt
     8101 tataagattt tctaaattta tatgaaaatt atcttcttct tttgcaaagt atttttctat
     8161 agaacaatta attttagata actctttttc atactctgaa taggctggag agagtaaaag
     8221 agcctgttta ggattaattg tatgtataaa tgatgaaatt agttctgtag ctccactgcc
     8281 aagtactata ttatctatag aacatttgca gtaattggat atagaagttt ttaagtctat
     8341 gtaatcaggg tcaggataca tagataccat atctatattg tttacaatgt attgtttggc
     8401 taaacttgat gtaccaaaag gatttatatt tgagctaaaa tccataaatt cttcctttga
     8461 aaagccatac ttactagata aactatataa attagctcca tgatttgtgc tcataaaagt
     8521 acctccaaaa ataaaattat ataatcgata aaaataactg attaatatat gtacatatag
     8581 attataatac aaaggaatcg ttattccaaa tttttaatgg aaaaagcaaa aaagtatgga
     8641 taagctgtta atttaggtct aaaaatgatg aaaaagattt taattgatat tttggtatgc
     8701 tataatatat aagttaatag aaggaaagcg ggaggataca aaatgtacaa agttacagga
     8761 aaaatgttga cagaagaaca aataaaagaa aaagtttatg aattaggaaa aaagatagaa
     8821 gaagacttta aaggcgaaga tttactagtg gtaggtatac ttaaaggagc tagtgtattt
     8881 gtttcagatt taataagatg tatagatttg gatgtaaata tagattttat gagtgttaca
     8941 agttatggaa actcaacaga gtcttctgga acagttaaaa tattaaaaga tttagatgta
     9001 gatattgaag gaaagaatgt gttgattgta gaggatataa tagattctgg tttaacatta
     9061 agtaatctag ttgcagcttt aaaaactaga aatccaaaat ctttaaaact atgtactcta
     9121 cttgacaaac ctcagagaag aaaagctaat atacctgtag actatgttgg atttgttata
     9181 gaagacaaat ttattgttgg ttatggaata gactatgctg aaaagtatag aaatttacct
     9241 tatataggta tagttgagga tgtagaatag atttaaatgt gaatcaaaag cactaatttc
     9301 taagggagtt agtgcttttt agttaattat ataaaatttt agtaaaggag acatctatat
     9361 ggatattcag ttaaatatat tattattttt ccaaaattta agaaatccaa ttttgaactt
     9421 tatatttctt gtctttacaa taagcacaga agcaccgtta ttaatattaa ttacaacaat
     9481 tatatactgg tgtataaata aaaaatgtgg acagaaaata ctgtttgcta taattggaaa
     9541 ttttgttgta aatttaggca tcaaagagtt tgtaaaagct cccagaccaa taggaataaa
     9601 aggtcttgaa tcattgagag tttctactgc tggtggttat tcttttccaa gtgctcatac
     9661 tcaaactgca acaacatttt gggttagtat tatgactata tttaaaaaga gatatcttca
     9721 tttaataggt gcagttatgg ttttaggagt aggattgtct aggctgtatt tagctgttca
     9781 ttggccaata gatgtgatag tgggttggat gttaggaata ttttttacag ttatatttat
     9841 aaaaatattt gactatattg atgataataa aaattatatt ttacttgtag ttttacttat
     9901 accatttata atagtagcta tatttctaaa tagccctgaa tattttgaaa aatttggtat
     9961 aatagtagga tttgtatttg gatatatggt tgaggataga tttgttaaat ttaatacaga
    10021 taataataac aaaaaaataa attttagcaa taaaaataag gggagtaaaa atataatttt
    10081 ttcaagaata tgtaggtttt tggttgggat agtaacaata ggaatgttat atattggcat
    10141 taagttgttt atttcaatgt taatagtcac tttaaatata agtgactcta ctatgagtat
    10201 aatttttatg aattttatta agaatacagt agttgttttt tatggaatag caggagctcc
    10261 agcattattt aaacttttaa aattaaattg attttaaggt atagagtcaa tagtttaaat
    10321 taaaaaaatt aagggtagaa aagagtgatt atttgaagaa ggtagtaata ataggtggtg
    10381 gaccagcagg gatgatagcc gcatcaactg catgtgagaa aggctatgat gtaacattaa
    10441 ttgagaaaaa tcataaatta ggaaaaaaac ttgcaataac aggaaaaggt agatgtaata
    10501 ttactaacgc ttgtgaaata gaggaattaa tagaaaatgt acctacaaat ggaaaatttc
    10561 tttatagtgc tttttatact tttacaaatg atgatgtaat aagtatgttt aataatcttg
    10621 gagtaaagac taaaacagaa agaggaaaaa gggtttttcc agagagtgat aaagcctttg
    10681 atattgtaaa tgctcttgaa agacaactta aaagtaagaa agtaaatata ttattaaatt
    10741 caaaagtaga aaaaataata tctaaaaata ataaaattga gaaagtaatt ttaaacgata
    10801 aaaaagaaat taagtgtgat tcagttgtag tagcaactgg tggattaagt tatccactta
    10861 ctggttctac tggtgatgga tataaatttg caatttcaca ggggcacaca attatagata
    10921 caaaaccatc tttaattggt atagaagtac aagaaagttt tacaaaagat ttagaaaaat
    10981 tatcattgag aaatgttgaa ataagggtgt ttaatagcaa acaaaagaaa gtttatagtg
    11041 attttggaga acttgaattt actagatttg gtttagatgg acctataatc aagtctgcaa
    11101 gttgtagaat gaaagataca aggaaagaaa attacactat attattagat ttaaaacctg
    11161 cattagatga ggaaaaactg gataaaagag ttcaaaaaga ttttcagaag tatacaaata
    11221 aaaaatttga aaaagcatta gatgatttac ttccaaaaaa actaatacct ataataataa
    11281 acttaagtga aattaatgca aatacggttg ttcaccaaat ttctagagag cagagaaaaa
    11341 acttagtcca tttattaaaa aatttaaagt ttacagtaaa gagatacaga cctattgaag
    11401 aagctataat aacttctggt ggtgtaaaag taaacgaaat aaattcaagc acaatggaat
    11461 caaaattagt agaaggttta ttttttgctg gagaagtaat agatatagat gcatatacag
    11521 gtggattcaa tttgcaaata gctttttcta ctggatattt ggcaggtttt aattgctaaa
    11581 aattacctga attaaaaatg tttaaaatca cataatatta tcagcaacac aaaataaatt
    11641 aaacagaagg aaggtaatca acatggcaag taacaataac aacaacagaa cagtagtacc
    11701 aggagcaaaa gaggctttaa accaaatgaa attagaaata gctaatgaac ttggtatgag
    11761 taattatcaa caagttgata aaggaaacct tactgcaaga gaaaatggtt atgtaggtgg
    11821 atacatgact aaaaaattag ttgaaatggc tgaacaacaa atggctggaa aaagcaacag
    11881 atagttataa ataattattt ataactattg gctattagtt tagctagagc tatctcaaat
    11941 atagaaagta atttctaaat tgagatagct tttttttatt aaattattaa atagatatat
    12001 ttttatgtaa taaattttat gtatttggat aaaataatac aaatgaatat gttacataag
    12061 gagtgatgta aaatgagtag tgtaaatttt gaggatttaa aaaacaaatt tattaatagt
    12121 gacttagatg aaaaaataag aatatataca acaacagaag gtttatcagt agaacaattt
    12181 aaagagctac taaaatatta tccaatacaa catttatcta aactagaaaa agcacttgga
    12241 tagcagtatt tagtccaaaa ttaaaaaggc aatttattta atattaaaaa tattttttat
    12301 taaataaatt gcctttttat aataggttta aattattatg cttttgatat ataattatag
    12361 attctctcat ttccaagttc aatagaccca gtattaataa gagtaacgat tccttctata
    12421 tctctagcag ttaagtattt atatggaatt aataaattat aaccagtatt acttagtaag
    12481 ttttttaatt gggttatttg agagatattg aaacattttg cagttttagt agattttttt
    12541 cttaagttag ataaattttc attttgaata tttattaaag tttctatata gtttctataa
    12601 gatatcttaa aatttgtctc attgactatt ttttcattaa gtatactact gataaaatgt
    12661 tgagacaata tattatctgc aaatttatct gctgttaatt ttccagatgt tatgcctttt
    12721 tgatattttc taagaatttt tttagtatag cagtatattt tatttctcgt ttctaaatcg
    12781 agttgttcta tttgcacaaa accacttcct tctaattata aaaatattta ttaaaaatct
    12841 atacttaact aataaagctt caataattta tttacagtaa atactaaatc ataaaatatt
    12901 tgttattttt tattattacc aagtataaaa aatattataa ttgtaaaact tacaaatata
    12961 tgtaaaatat ttaggagtga tgaaaatgtt aaatgggata ataaagcttt tttattctga
    13021 aattgtaaaa acaaagatta ataacctaga ctcaagtgga tattctttaa gagatgaatg
    13081 taaagaaatc agaaatataa ctctaaagat agatagcgta ttttttaagc ttttaaagtt
    13141 ggcttttggt tcaatactgc ttatgatagc ttttaattta aacatgattc taggtatagg
    13201 agtgttttta attggtgtag tttatatact atataaaaga aatatggaag aacaagtaaa
    13261 agagcatata aataatgtga aaaataatat cgacatctct aaaataactt ttatggatga
    13321 aaaagggaga acaggtctaa atgcattgat tacattactt cttataggat tattgacagg
    13381 atttaactgg atattagttg ctagttttgt cgttgttttt gtttttacta taaagaatgt
    13441 atgttaaaat actaataaat agtttaatat atagggggag aaattgagta tgactaatgg
    13501 cagttataaa atgaagaatt tagtggagat atccgctatg gtgacggaat ctacagattt
    13561 ttttaatata aaagatgaca taatagaaaa aatgttagaa gttgttcacc ctacaaaggc
    13621 atgtgtaaat ctattttata aaaatgattc taaatatgcc tacttagtat gttcgcaaac
    13681 cttagaatat gtacctcaaa tttttccaat aaatagtctt aaaggtgcaa aaattgattt
    13741 tgacacatat ccagagtata tacatgaggc tgtaaatgaa aaaaagataa tatatgtgga
    13801 aaatgtattt gaagattcta gagcagaagg tgaacgagat ttagcaaaag ctgaaggtta
    13861 tattgggaga atagtttttc cttttataat aaataatgta gttgttggat ttatgacttg
    13921 ttttttaaaa gaggatgatt atataacaga acaagatata gattttatat catctgttgc
    13981 atcactctta agtttatcaa tagaaataac taataaaaat aataatgtac aaattttaat
    14041 tgataaacta agaggctcaa taagtgccat aaatgaagct actaaaaaac tataccttaa
    14101 taagagtatt gatggctttt tggaaaattt aagtaaacaa gcgtgtaata ttactaatag
    14161 taaagaagct ttaataatta taaatagtca tcataataag gatactatag ttagttgtta
    14221 taatgttgag caaaaaaaga aaacaaattt atatcctatg attgatatgt ttttaaagaa
    14281 agagtcatta gggggatacc taaataattt aaaaccatgc tcaaaaaagg gcataaattt
    14341 ggaaagttat atatattaca aacttcagga taaaaatgag actataggat gtatagtttg
    14401 tgcaaatagt aaaaactata ctagtgatga tttaaatatt cttagtatat tgtccaaaca
    14461 agtttctgtt gggatgcaat tatatgaaca taatgaaaag gaagtaaaac acaaagttct
    14521 tgaaaatgag ttgaacatat taaataaaca acaacagctt attatggata aaggtaaaat
    14581 ggattgtaat gatgagaaag aactttattt ttaccataaa ccagcaaggg tagtaggtgg
    14641 agatttttat tatgctacaa aagtggatga cgaacatata gtttatattg ttgctgatgt
    14701 aatgggacat ggaatggtat caaattatgt tgttgcaatg ataaaaggtg cttttaaagt
    14761 attatgcaat caatacaata caccaagaga aataatgaca aatttaaata ggatgttata
    14821 tgatgaattt gacaagatgg gagtatttac aacttgtttg ataggtatta tagatactaa
    14881 agaaaatatg attactgtgt ctaatgcagg tcactatagt cctgtagttg taaagaaaga
    14941 tggcacaata gaaaataatt taaattgtaa aaagggcata cctattggaa taatgaaaga
    15001 tgctacttat gaaaataata ctctttcaat ggaagattat gcaatggtat gtatgtacac
    15061 tgatggaatt ttagagataa aaaattcatc aaaagaagag tatggtataa acagattaga
    15121 aaactttcta aaagaaaatt ttagattaaa tcaacagtta atagtagaaa atctaaaatt
    15181 ggatttaaag aatttctcat caaaagataa ttatgatgat gatattttaa tagttatgtt
    15241 aaaggatagg tagattgatt atggattcaa acgataaaaa aggacttaaa agtacagaga
    15301 ttataataat aaatggaagt ccaagaatta ataaaaattg cagttttata gcaaataaag
    15361 taaaagctat gctagaagag tgtgggttaa gttgtaaaat atttaatatc tacaatatgg
    15421 atattgaata ttgcacagca tgtggatttt gtgaaaaaac agggtattgc aagtttaatg
    15481 atgatatgaa gccattatat aaaatgtttg atgattcaaa aggaactata gtaataagtc
    15541 ctgtacattt tgattcaata tcagctaaat taaagactgt tgttgataga actcaagcta
    15601 tatatgcaag taaatatgta ttaaataaac cttcaattga tagaaataag aaacggatag
    15661 gaatgtatat tgcagtgggg gggagtaaat catatgattc acagtttaaa ggtggacaga
    15721 ttgttatgga ttttttcttt aagagtataa atactaaact tttacacaat atatatttaa
    15781 acaatacaga tgatgtatct tatatagata atcaagaatt taaagatata atatat
//
LOCUS       DAMJVX010000080        15717 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.084, whole genome shotgun sequence.
ACCESSION   DAMJVX010000080 DAMJVX010000000
VERSION     DAMJVX010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15717
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.084"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            150..1367
                     /locus_tag="PC407_002208"
     CDS             150..1367
                     /locus_tag="PC407_002208"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HDF2497652.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1462..1842
                     /locus_tag="PC407_002209"
     CDS             1462..1842
                     /locus_tag="PC407_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2497653.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2126..2608
                     /locus_tag="PC407_002210"
     CDS             2126..2608
                     /locus_tag="PC407_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2497654.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVNLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2629..3444
                     /locus_tag="PC407_002211"
     CDS             2629..3444
                     /locus_tag="PC407_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2497655.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3495..5045
                     /locus_tag="PC407_002212"
     CDS             3495..5045
                     /locus_tag="PC407_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2497656.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5142..6494
                     /locus_tag="PC407_002213"
     CDS             5142..6494
                     /locus_tag="PC407_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2497657.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAESLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKVFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6625..7383
                     /locus_tag="PC407_002214"
     CDS             6625..7383
                     /locus_tag="PC407_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2497658.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIYNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7430..8149
                     /locus_tag="PC407_002215"
     CDS             7430..8149
                     /locus_tag="PC407_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2497659.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8273..9673)
                     /locus_tag="PC407_002216"
     CDS             complement(8273..9673)
                     /locus_tag="PC407_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDF2497660.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9677..10585)
                     /locus_tag="PC407_002217"
     CDS             complement(9677..10585)
                     /locus_tag="PC407_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2497661.1"
                     /translation="MKGDNKVTEKQRLIINIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10846..11493
                     /locus_tag="PC407_002218"
     CDS             10846..11493
                     /locus_tag="PC407_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2497662.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11818..14457
                     /locus_tag="PC407_002219"
     CDS             11818..14457
                     /locus_tag="PC407_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2497663.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14515..15618)
                     /locus_tag="PC407_002220"
     CDS             complement(14515..15618)
                     /locus_tag="PC407_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDF2497664.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSSK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
BASE COUNT         5917 a         1895 c         2743 g         5162 t
ORIGIN      
        1 tttattgtat gcaattgttg tatttttagg ttttttgtga ttaattaaaa aattataaaa
       61 atcgtttaca tgttgatttt taaaaaaaaa agatatagaa ttatatagaa agtaataaac
      121 aaaaattgga tttacgggag tggaatgaaa tgatgaatgt taccttaaca gatgtgaaag
      181 aggctagaga gaccattaag aacatagtaa aaagaactga cttacttgaa agtgttaaac
      241 ttagcgaaaa aacaggagca aatgtgttct ataaatgtga gaatcttcaa aaaacaggtt
      301 cttttaaatt aagaggagct tgtaacaaaa tagcgagttt aacagatgaa gaaaaggcaa
      361 atggagttat agcttcaagt gcagggaatc atgctcaagg agttgcattg ggagcaaaaa
      421 tgactggtat aaaatctaca atagtaatgc cagcaactgc gcctttagca aaagttagtg
      481 caactaaagg ttatggagca gaagtagttt taaatggtgc ggtttatgat gatgcatatg
      541 caaaagctgt tgaaatacaa aaagaaacag gagcaacttt cttacatcca ttcaatgata
      601 agtatgttat agcaggacaa ggaactatat ctcttgaaat tttcgaacaa ttagacaata
      661 aagttgatac tatactatgc ccagttggtg gaggaggaat catctctggt gttgctgttg
      721 cagcaaaagc attaaatcct aatgtaaaaa tagtcggtgt tcaaactgca aatatacctt
      781 caatgaaaga gtcaataaaa aatggaaaag taacaacagc atttaatgat actacaatag
      841 ctgatggtat agctgttaaa actccaggag atttaacttt tgaaataata aatgaattgg
      901 ttgatgaaat agtagtggtt gaagagacag aaattgcaga atcaatatta tttatgatgg
      961 aaagtcaaaa aatagtttca gaaggtgctg gtgctgtatg tacagctgct atattaagtg
     1021 gtaagtatgt accagcaaaa gatgaaaatg tagtttgtat aatatctggt ggtaatatag
     1081 acataaatac gttatataga ataataggtg ttgctttagc taaagaagga agaagatatt
     1141 catttagtac aattatggaa gataagccag gaaactttgc tgagttaact agaataataa
     1201 gtgagaatgg tggaaacatt ttaagtgcta atcaaggtaa attatcagca ggggaagctc
     1261 taggaaaaca aagtgcggaa tttatattag agacaataga ttatgaccat atagctagaa
     1321 taaagaaagc tatagaagaa aaaggtttta aaattataga attataataa aaagtgtata
     1381 taatatacaa gaaaaatagt ataatagtgt atgaagatac attttatgtg tattagatta
     1441 acgaattagg aggattttaa aatgaaacat gaagtaatac atactaatga tgcaccagca
     1501 gctttaggac catattctca agctataaaa gctggaaact tattatttgt atctggacaa
     1561 gttcctcttg taccagagac tatggaagta gttgaaggag atgtacaagc tcaaactgct
     1621 caatctttaa aaaatcttaa agctatatta gctgaaagtg gagctgattt ttcaaatgta
     1681 gttaagacta cagtatttat aaaagatatg aatgaatttg gggctataaa cgaagtttat
     1741 gctgaatatt ttggagaaaa taaaccagca agagcttgtg tagaagttgc tagattacca
     1801 aaagacgtta aagttgaaat agaagtaata gctgtattat aaaaatacaa tatttttaat
     1861 ttgaaaaggc tgaaaaacta tattctagtg attcagtctt tttataaaaa aataaatcac
     1921 aataaagaac atgataaata ttgtcgaatt aaaaaataaa attaaaataa atattgcaat
     1981 atttttgaga aggttttata atgagtatat aatttgaaac gtgtaactat taaattaggc
     2041 atgagttctt ggatattttt atcctgagga ttcatgcctt ttttgtattg aaattcaaaa
     2101 atctatttaa taaggaggaa gtattgtgtt aaattttttc aagaaaaata aaagctataa
     2161 gttacatgca gtagtgagtg gtaacagcat taatattgag aaagtaaacg attctgtgtt
     2221 ttcaaaaaag ttaatgggag atggagttgc aattattcct aatagtaatg ttgttgtagc
     2281 cccttgtaat ggtaaagtaa ctgtgcttac agaatcaaag catgcctttg gtatggtctc
     2341 agatgagggt gttgaaatat tagtccacat aggaattgat acagttaatt tacagggtga
     2401 ggggtttaaa aatgaagtgt ctcaaggaga tactgttaaa aaaggtagtc caataataag
     2461 ttttgaaaga gaaaaaataa attctcaggg aattgattgt acaacaataa taattgtatt
     2521 aaatcatagc gaattttcag aaataaattg catggtagaa aatgaagttg ttgcagggca
     2581 agatactgtt attgaaatta tgaaataata ggaagggatt aattcaatat gagagtagta
     2641 aaaatcttta ataataatgc attatctaca gtaactaatg ataggaaaga agctatttta
     2701 ctaggtcttg gtataggttt taataaacgt ccaggagata aagttaatga agataaaata
     2761 gaaaagattt attatgttca agaccacatg caaacaaaat ttttagaatt attaaaaaat
     2821 gttagtccag aagtgatgga tgcttcacaa caaattataa gtcttatacc aggaaatgag
     2881 ggaaaattta ataataaagg gatattatct ttagtggaac atattagttt tgcaatagat
     2941 agaatgaaaa ataatgtttt tttacctaat ttaatgcttt tggatataaa gatgatgtat
     3001 tcaaaagagt ttgaattagg agtaaaagct ttagaaatta tatatagagt atgtcatatc
     3061 catttgcctg aagatgaagc aggttacata gctttacatt ttgtcaattt acaatcaaat
     3121 gataatttgg catatgatac tttaaaattt gtaaaaggta gtattgattt gattaaagaa
     3181 tgttatggat tagaattaga cgaatcgagt ttaagtacat tacgttttag aactcatctt
     3241 aaatttcttg ctcaaagaat ttttaagaat gaaatttgtc aggatgataa aatgattgaa
     3301 atgtatgatt atttaattaa caatcatcct aaaaataaag aatatttaga aaagttaaat
     3361 gtatatattg aaaaggagtt taggtataaa ttagataaac ctgaaaaaat ttatctacta
     3421 ctacacctaa caaaaatctt ataagaatga gatgaattag taatagaaat atataatttt
     3481 agggggggat aagattgaaa gaaaaactac ttggaggact ggaaacattt gcaaaggcaa
     3541 tggttcagcc attaatgtat ttatcgagtg caggaatatt aatggtggta ggtgtactac
     3601 ttacaaatga tatgttatta ggagtacttc cattttttaa atgggagcca attcaaatat
     3661 ttggaagcct aatttataac tgtataatgg ctattattaa taatcttgct gttatatttg
     3721 ctataggtat tcctgctgca ctagcaaaaa aagagaagca caaagcagcg ttaataggat
     3781 ttatgagtta tataatatac cttacagctt ccaacactat gttaacatca ctaggtcaac
     3841 tagcaaaaga aagcaagatg cttggactga tagggactgg acaagctact attttaggta
     3901 ttcaagttgt agatacaagt gtatttggtg gaattatttt agggtgtata gctggatatg
     3961 tatttaatcg tacaagcaac aaaacattta aaggagcttt acagatatac tcgggagcaa
     4021 attttagttt tatatgcatg ttctttgtat ccattttaat tggagttgtg agtaacttta
     4081 tatggccatt agctcaacaa ggaatagctt ctcttgcaaa tgtcatcaaa gatacaggta
     4141 catttggttt attcttatat ggttttttag agagaatatt agtaccaact ggacttcatc
     4201 atttaatcta tacaccattt cagttttctg atttaggagg agtgcttaaa gttggtgcag
     4261 atacagtttc aggagcctat gctatagtta tgacagaaat gaatttacca gttgagcgtt
     4321 tctcagatag tatatactac atggcaactg gatttacaaa gatgtttggt tatataggta
     4381 taggagctgc ctttatttat actgcatata agcaaaataa gaagaaagtt aaggcaacga
     4441 ttattccact aataattacg gcagtattag cctctgtaac agaaccatta gactttatgt
     4501 ttgtattctg tgcaccactt ttattctttg tacatgcagt tatagctggt ctttttatag
     4561 ctttattaaa aatatttgat gttactgcat tatgtggagg gaacttatta gtatcaatta
     4621 taatgaatac tacagtaggt gtagaaaaaa cacactggcc agtaatgatg atattaggac
     4681 ttgcacaaat tattgtatat tttgtagtat ttagtttttt aattaagaaa tttaattata
     4741 aaactccagg tcgtgaagat gagagtacag ctacaggcga agaacaatca aaacaactta
     4801 atttagatgc aggtatagaa aatataattg atgggttagg cggaaaagaa aatattaata
     4861 cagtagaaaa ttgcatcact agattacgtg ttaatgtaaa agatgaaagt aagattaatg
     4921 aagatataat taatttaacg ccaaatagcg ggattgtaag gaaaggaaaa gatatacaaa
     4981 taatttttgg acttcatgtg catgaggtaa gacgtgcagt agaagatttt ttagaagctt
     5041 attaaagata tatatttata tagcaagggt aaataacagc tttaagtgct gtttactatt
     5101 tattaaagat acatataatt tatagaggag gaataattat tatgattatt actattgttg
     5161 gtggaggaag cacatttact ccaggtattg ttaagtcaat tgcgttaaga gaaaaagagt
     5221 tagagcttga ggaaattcgc ttatttgaca ttgataaaga acgccaagat aaagtagctg
     5281 tagttgtcaa ctggatacta aaagaagatg ttaaaacaga tgttaaacta actgtaacta
     5341 attgtgaaaa agaagcgtat acagatgcta catttatatt tgcacaaatg cgtgttggta
     5401 aatatgaaat gcgtgagcag gatgaaaaaa ttccattgaa atatggttgt gtaggtcaag
     5461 aaacttgtgg ttgtggaggg atggcatacg gactgcgtac aatacaacca atgataaagg
     5521 ttattgatga tgtagagaaa tatgctaaaa aagattattg gatattaaat tactctaatc
     5581 ctgctgcaat cgtttctgaa gcttgtcgta agttaagacc aaatgcaagg attataaata
     5641 tctgtgatat gccaatagct attatagata tgatagcgga gtcattaaat atcaatgacg
     5701 ttcataatat acgctatgat tattttggtc taaatcattt tggctggttt acttcaatag
     5761 attataaaca tagagactta atggaagaga ttaaggaata cataaaagaa aataaaatat
     5821 tattaccaaa atcttatata gaaaacttaa ataaaagtcg tagtacagga aatcgccatg
     5881 ctgtaagtag ctggatacat gtttgggaat ctgtttatac aatgttagaa agcttcccaa
     5941 aatatctgcc aaacacttat ttaacatact atcttttaag taaagaaaca gtggaacatt
     6001 caaacataaa tcgtactcgt gcaaatgagg ttatggaaag ccgtgaaaaa gacttatttg
     6061 aaggtgtgag acattactta gaaactggag aaatttctga aaaagtattt tatgcaggtt
     6121 cacatggaga ttggattagt gatttggcag tttcaattaa aaatgataca cgtagtcgtt
     6181 ttttagttat aactgaaaat cgtggagcta taccaaacat gccatatgat gcaatggtag
     6241 aaattccagc atatattggt aaagatggtc cagaagttat tgctcgaggt ccaatacctc
     6301 tattccaaca aggattaatg atgcaacaac ttaacagtga aaaattgata gtagaagctt
     6361 atatagaaaa tagttatgaa aaagctttac aagcattcac attaaataaa actattccaa
     6421 gtatgaatgt tgctaaagaa attttggatg aaatgataga agttaataag gaatactggc
     6481 cagaattaag atagtaggat aaaagaaagg gtttttattt aataattaga tatgttttca
     6541 gagtctttta tcaaaatgta ttcacaaaca tatggcaata tattaaaata aagagtgata
     6601 tttttagaag aatggggtga tgcaatgaac attataaata aaattttttg gcaagaagat
     6661 aggattggca tgattacaaa taatttagag gcagatacac atagccactg catgttacaa
     6721 ctgttcttag ggatagaaga cagcattgaa ataacagtca atgaaaaatt agttaaatgt
     6781 aattgtatca tagtagataa aaatatctct cattcatttt cagcaagaaa aaaagtttat
     6841 tactctgcta ttattgaacc tacatctata tatgcagaac agcttacctc aaaaatgaat
     6901 gattttggct actggatatg tgacaatgat ggattagaga aattacgtca acaaggaact
     6961 tttttgatat ataactctag taaagaacaa tatttaagat ttatggaaat gcttaataac
     7021 tatttgaata tacctataac tctcaaacat tatgatgaca gaattactga attgctcaat
     7081 ttattacgta cttgtaattg tgataatcat actatttcaa gttttgcaga taaagtgtcc
     7141 ctatcagcaa gtagattatc acatctgttt aaagaacaaa tagggatacc tttaaaaagc
     7201 tatatcttat ttcatcaaat ggagtgtgct tttagagaat tactttctgg gaaaaatgtt
     7261 accgaagctt ctatgactgc tggattcgat actccatcac actttgcagg gacagttaag
     7321 cgaatgatgg gaatgcctgt ttctttatct ttgaaagata gcgaattttt gaaagtatat
     7381 taaatattct cgttatataa tgaagttgta ctaagaaagg ggctttgtta tgaataaaga
     7441 atatttagag gaaacaaaaa tgttaaatta taatgaacca caacttaagc ttttggtatc
     7501 atcaaagaat tggttggaat tagatgattt tcataaaata aaatctattt atgagtttgt
     7561 acagaatgat attttatttg gatataatgc ttctgatatg ttaagcgcaa cacaggtatt
     7621 aaatgatggt atgggacaat gcaatacaaa agctactctt ttaatggcat tattacgtgc
     7681 agtgaatatc ccttgtaggc ttcatgcttt tgatgtaaca aaagattttc aacgaggtgc
     7741 tacatccaaa ttgatttcac tattagcacc aaaatatatt cttcatactt gggtagaggt
     7801 attttatcaa gataggtgga ttgctctgga gggtgtcata actgataaaa aatatttaga
     7861 agcaattcag aagaagtttt ttaatcatgg tggaacgttt aaaaagtatg caatagctac
     7921 aaatgatttg aaaaatacat ctattgattg ggatggtaaa gatactttta tccaaaaaga
     7981 agctatcgtg tatgattatg gtatttttcc atcaccagat gtattttttt ctactcattc
     8041 acaacatatg tcaaaattaa aaaatttcat atatgtacat cttatcagaa aaataatgac
     8101 aaaaaatgta tgtaaggcta gaaacaatta tattgataaa aacgaatgag tgtttaagag
     8161 gtaactatat aggaaatata tagaataatt aaaactgtac tggttaaaat gaataaaaag
     8221 gagttatcat agagcaaact taaattgttc ataggataac tccttttctg tattaacttc
     8281 taataatttg atgtcttttt ataaaacata tagatacaat taaaccagta aatgcaagag
     8341 tagctgctat tgaaaatgca acatctattc catgaatgtt agacagcatg tttgaactca
     8401 tatgagcttt cttagtagca cttgtcatta tagtaataag tatagcagaa cctatagctc
     8461 cagatatctg acggagtgtg ttattaattg ctgtggcatg agatataagt tctctatcta
     8521 atgttttaat tccccatgta gttaatggca ttaaaaccat tgaaatacca atcatacgaa
     8581 tacaatacat caatgacaca tgaattaaag atgtatctct gcctaaaaaa gcgaaggata
     8641 aagttcctaa aaacaaacat cctgttccta atattgatag agcatgaggt ccatatttat
     8701 ctaaatgacg tcctgcaatt ggattgagta caaccatcaa tatagcacca ggtaacatta
     8761 atgaacctga agataatgca gaaaatcctc ttacagattg aacgtacaga ggaatcatta
     8821 atgtagcaga catcattgag gcatatacaa tcataattaa tattgtagaa ataagaaatg
     8881 gtttattttt aaaaaccctc aattctaaaa aaggttcttt agattttaat tgacgcaatg
     8941 taaataaaat taatgacata attcctatta atattggtaa atatgtggca atattagtcc
     9001 acccataatt accttgatta gtaaatccaa ttaataaacc accaaaacct agagatgata
     9061 gtactattga aggtatatct aactttagtt tttgagtttc cccaacattt cttaatagaa
     9121 taaatgatag tataacatct aatatagcaa taggggaaag aaacagaaat aaactatgcc
     9181 aaccaaatga atctactaac catcctgata aagttggacc tattgctgga gcaaatccta
     9241 ctgtaatacc aactaaactc atagctgcac cacgtttttc ctcagggcat aaatagagta
     9301 taataacttg taaaagtggc attagagaac cagaacccat tgcctgcaac acacgagcaa
     9361 tcaacatagt tgaaaaatta ttagaaaaaa gtgcaataat acatcctata gaaaagatag
     9421 acattgatgt aataaataac tttctagtac taaatcgatt tattaaataa gctgttgtgg
     9481 gtacaataat acctgcaata agtagataaa ttgttgttaa ccactgacca atatcagcag
     9541 taattttaaa atctgacatt atattaggta atgctgttga caatagtgtt tgactgagag
     9601 aacttataaa agcaccagtc attacaattg ctattattaa atttcctttt ttattgttac
     9661 tttgggtttc cataatttat aacgtctcct tttttaattg atttttaatg ctgttcaata
     9721 aaagtacttt tggtcttaaa gtgtcttctt tttctaaata tgcaatcatt gcatccataa
     9781 ttaaagaatc atattgttga ttttttccct cgacgtataa tttttttatt gcttctagta
     9841 atttaggatt ttcaaggaga gaagtatcag ttttgattag ttcttctata tcttgtatcg
     9901 ttttaaaaaa tagcgattta ttatgaaggg aaaagtaaac ttcatcttct tgtttatgta
     9961 aaaacattga tttttcaatt aaaggattta gattatccat attttctaca actaaatcta
    10021 gaatttgtat agtaaatttg gcaataaagt atacatttat ttctcttttt tgtacatgaa
    10081 taatgtatat aaactgatga attagagcat ccaataagaa aactaaatca aatataattt
    10141 tttctatttt atcaccatag ttatcaaata agcttaattt gtattcattc aataagttag
    10201 ctcgtgattt tgtttgcaat ttacttgtag ccttacgttg ctcttcagaa aaattttctc
    10261 ttatataggt atttatggta gttctagaaa aaatattttt ccatacagca attatctttc
    10321 tgtttaactt ttctgatgat ggaatgtcat tattttcgtt tatggaatgt ataatttcta
    10381 aaaacttttc atcataataa ttgattattt cgcagataaa actttcctta gtttcaaaat
    10441 gcttgtaaaa agtagcttta gacattttac actcttttac aacatccgat attgaagtat
    10501 tttgaaaacc tttttggtca aaaatttttt gtgcaatatt tataatgagc ctttgttttt
    10561 ctgtcacttt attatctcct ttcacgcaaa aacgtttagt actatatagt acctacaagt
    10621 tttaataaat atattataca tcatttggaa aaaaaatcaa tatatagact gtaatatgaa
    10681 atttttgaaa aatttgttgt tatttttagt cgtataaaaa agaaacttaa gagtttctta
    10741 agaattgttt aaagaagttg caataaatta gtggtatgat tatagtatga aagagaatgt
    10801 cttgaaattt ttctgtatca aattccaatt atggaggtgt agtatatgaa aaagaaaatt
    10861 tgtgtagcaa taatttttgc tctatttata gtttttgtag aaatgtttat tgatagctct
    10921 agcgaaggaa gtaaaattat gacagctgaa ttaagtaaat acaggaaagg cgtaaatgta
    10981 ttatcaataa acaatattat agtagcgaat aaaaaatata gtttgcccaa agattattct
    11041 cctcaggaaa gcagtgaagc gagagatgct ttttataaaa tgaataaaga tgctcaaaag
    11101 tcaggattaa atttaaaagc ttttagtaca tatagaagtt atgaatatca agatagatta
    11161 tttaaatcat atgtaaaaga acatggagaa aaagaagcaa atagattttc agctaaacct
    11221 ggagagagtg agcatcaaac tggacttgct tttgatattg gaggagatga ccagtcttgt
    11281 tgggcaaata aaaagttcaa caatacaaaa gaagctaagt ggttgtatga aaatgcatat
    11341 aaatatggtt ttatacttag atatccagaa ggtaaagaga atataacagg atatatgtat
    11401 gaatcttggc actatagata tgttggaact gaacatagca aaaactttgc aatgaataat
    11461 ttgactttag aagaatattt acatataaat taagagatgg tttgttaaaa ttaggttaaa
    11521 ataaaaaata acctagacta ttagctgtgt aaagtgatat aatgtaaata caatttaaga
    11581 taactactcg aaggggagta gcttacacga aagtggtaat taatcgtcaa gacagtagaa
    11641 atactcggtt aattagcaag ggattccttg atagcaagac cttcaaaata agcattaggt
    11701 atgtttgttt tggggtcttt ttttattcaa ggaattttta tttaaaaaaa tttaaatttc
    11761 aagtatacat aagagccccc aaacataaaa taaatttatt gaagaggagg gcaacaaatg
    11821 tcttacaaaa gtacttatca agaagtaatt aaaaatttag actcaagttt atctggtttg
    11881 agtaaagaaa gagcagaaga gttgttggaa aaaaatggag ctaatgaatt aaaagaagca
    11941 gataaggttc ctacttataa gcttttccta gaaagtttta aagacccatt agtaataatt
    12001 ctattgatag ctgctttagt acaaatattt ttaggtgaaa cagtggagtc tataataatt
    12061 tttgcagtta ttattataaa ctctgttctg ggtgttgtac aaacaaaaaa agcagaaagt
    12121 tcattagaaa gtttgaaaaa tttatctgca ccaaacgcaa aggtaataag agacaataaa
    12181 aaaatgacaa ttccagcaaa aaatctagta gttggagata ttgtattctt agaagctggc
    12241 gattacatac cagcagatgg aaggcttata gaagcacaat ctctcaaggt tgtagaaggg
    12301 atgttaactg gagaatcaga accagtttta aagcatacag aaaaaataag tgaagatgta
    12361 agtttaggtg accagaaaaa tatggtattt agtggtgctg tagttgttta tggtagaggt
    12421 tcttttgtag tgactggaac aggtatggat actgagatgg gtaaaatagc tgggctttta
    12481 gaaagtgctg aaaataaact tacaccattg caacaaaaaa tagaggattt tagtaaaaaa
    12541 cttggtgtag gtattgtaat acttgctctt ttaatattta taattcaagt agcaagaaat
    12601 tactttatgg ctggaaatgg agaaatgaca caaattattt tagattcatt catgttctct
    12661 gtagcagtag cagtagcagc tataccagaa gctctatcat caatagtcac tatagtattg
    12721 gctgttggaa caaatagtat ggcaaaaaaa caagccataa taagaaaatt accagcagta
    12781 gaaactttag gttcaacaag tgtaatatgt actgataaaa caggtactct tacacaaaat
    12841 aaaatgacag tagttgatta ttatatgtat ggaactaata atgatgagtt agataaagaa
    12901 aaagtaaact attcttatca tgctaaatta ttgactatgg tttctacatt gtgtaatgac
    12961 tctcatataa ctagtgatgg taaagaagtt ggtgacccaa cagaagtggc tttaataaat
    13021 tattcaagta aaaatgattt agattatgat aaattaagaa gtaagtatat cagaataaat
    13081 gaaataccat ttgattcaga cagaaagctt atgtctactg taaatagtat atatggagat
    13141 tatataatgt ttacaaaagg tgctccagat attgtgttta gtagatgtaa atatgctttg
    13201 aaaaatggtt caaaagtaga tataacagat gagataatag aagaatataa acataaaaat
    13261 gaagagtttt caaatagagc acttagagta ttagcttttg caataaaaga tgtcccagaa
    13321 gaagatttta taccttcaat tgatgacgaa catgacatga cattggttgg aataatggca
    13381 atgatagacc caccaagaga agaagtaatg gatgcagtaa aagaggcaaa gagtgctggt
    13441 ataaaaactg ttatgataac tggtgaccat aaaacaacag cagcagcaat tgcaaaagag
    13501 attggtataa tggaagaagg agacttggct cttactggaa aagagcttga tgctttgagt
    13561 gaagaacaac tatatgaaaa actagaaaat ataagtgtat atgccagagt ctcgccagaa
    13621 aataaaattc gtatagtaaa agcatggcaa cataagaata atataacagc aatgactggt
    13681 gatggtgtta atgatgcacc tgcattaaaa caagcagata taggaattgg tatgggaagt
    13741 ggtactgatg tagcaaaaga tgcttctgct atggtattgg ttgatgataa ctttgcaagt
    13801 atagtaaatg ctgttgaagt tggtagaaca gtatacagta atataataaa atcaattact
    13861 tatctatttg caggaaactt aggagctata gtatccatag tatttgcagt gtttgcaggt
    13921 tggtcaaatc catttacagc tttacaactt ttgtttataa acttagtaaa tgactcttta
    13981 ccagcaattg cattagggtt tgaaaagtca gaaaaaaata taatgaataa acctcctaga
    14041 cacccggatg aaagtatact agcaggagga acattgaagg cagttgctat aagaggttgt
    14101 ataataggtg tagttacaat aattgcacaa tatataggat tacaagtatc tccagaactt
    14161 ggagtagcaa tggcattttc aacattgata ttagctagaa taatgcaaac attacctgca
    14221 cgttctaata atcaaacagt aataaaatta ggttttttca gcaataaata cgttatagga
    14281 gcagttttag tctgtcttgg gctttactct ttaacactaa taccagtgct aagaggaata
    14341 ttctctatac caacaacgtt tggattgtac cagttgggga tttgcctagg gttagcatta
    14401 atttcatcaa tattaatgga acttaacaaa ttaataaata gaagtgaagt aaaataatca
    14461 aaaaattaaa gggcatattg attttaacaa tatgcctttt ataaatagtc taatttattc
    14521 tatattatcg attatttgga atgcttttat aacttcaggt aattcttttt cttcaggcat
    14581 tctaaatctt atgaaatttt tatcaagtcc agtaaaatca cttccagata taactcttat
    14641 ttttaattta gcaaactctt gttgtaaatc tatgtccttg tttttatgtt caactgtcat
    14701 aattgataca gtatcactag tttcagctat attaaggttt ttccatggaa ttagtaattg
    14761 attttttata tcttttgttt tttcttttaa ttcttctata aattgaacat catctaataa
    14821 atttgctgca atactacgag atatttcaga cacttcgtaa ggagtagaaa tctttttaat
    14881 agggctaaca agttgttctg gaagaactgc atatcctgct cttagtcctg ctaaaccaaa
    14941 tccttttgaa aatgttttta aagcaattac attatcataa ttatttagaa gcttgacagc
    15001 tgaattttct tttggcatgt attcaccata agcttcatct accataactg ctatatcata
    15061 tttagctgct tcctttacta tattttcaat tgatgataaa ggtataattt gtcctgtagg
    15121 attgtttgga ttgtctatgt aaattacttt atattctgga tttatttttt caataaattc
    15181 tttttcatta aacttgaaat tatcctcttt ctttaaaaga acataatcat aagttgcacc
    15241 atgcattttt atatctgtct catattctga gaactgaggt gaatatccta aaactttatc
    15301 tcccttttca ataaatagtc tatttaaaag gtaaataaca tgtatagaac catctgccaa
    15361 acaaattctg tcagtatcta atgctataaa atctttccaa taatcgacta tattatcttt
    15421 taaagcaatt gaatgaggat atcctcttat catatcaaat tttaaagcat taaaagcttc
    15481 tacagctttg cttgagtaag acacggtatt tataccttca ccacagtcta tctcgaatac
    15541 atcttctgaa tgtccagatt ctattgcata actttttttc aactgcattt caatagcttt
    15601 gttaattttt agtttcatac tttatcactc cttatttagt caataataaa tgttataaaa
    15661 gttgctagat ttaaaatatt atgaaaatat ataatagtaa aaaatgaata taaatat
//
LOCUS       DAMJVX010000081        15614 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.085, whole genome shotgun sequence.
ACCESSION   DAMJVX010000081 DAMJVX010000000
VERSION     DAMJVX010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.085"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(52..912)
                     /locus_tag="PC407_002221"
     CDS             complement(52..912)
                     /locus_tag="PC407_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDF2497665.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(1229..2557)
                     /locus_tag="PC407_002222"
     CDS             complement(1229..2557)
                     /locus_tag="PC407_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2497666.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2705..3034)
                     /locus_tag="PC407_002223"
     CDS             complement(2705..3034)
                     /locus_tag="PC407_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2497667.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3058..4347)
                     /locus_tag="PC407_002224"
     CDS             complement(3058..4347)
                     /locus_tag="PC407_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2497668.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4363..4689)
                     /locus_tag="PC407_002225"
     CDS             complement(4363..4689)
                     /locus_tag="PC407_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2497669.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4824..5453)
                     /locus_tag="PC407_002226"
     CDS             complement(4824..5453)
                     /locus_tag="PC407_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497670.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5668..6684)
                     /locus_tag="PC407_002227"
     CDS             complement(5668..6684)
                     /locus_tag="PC407_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2497671.1"
                     /translation="MVKAVGDIIGKCSAISFDMKLDKSYDEVIVDFKNLINNIDNKDV
                     LLFTDMGSLNSFDEIIKKEKKCEVRVIPMVTTLTVLEAVQKANMGLPLNDVYNSITNT
                     RKYYFGTNEIQNKENLSKTIIIASHVSEGVDNKTRKILEEKMSRYLDGIDIISVPYKT
                     EKDLSLNITKLKESSNIVAVINEQRINIRGIDYISKKDIDKDENINKLKNIIKISIGY
                     DDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLVFNIEKKYDKVIGLMMHLAFMVDGLI
                     GNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDKKYNIEINEKECYQILLILEYAEIIE
                     KDYQ"
     gene            complement(6665..8323)
                     /locus_tag="PC407_002228"
     CDS             complement(6665..8323)
                     /locus_tag="PC407_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2497672.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERDSKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASNGKSSW"
     gene            complement(8682..9626)
                     /locus_tag="PC407_002229"
     CDS             complement(8682..9626)
                     /locus_tag="PC407_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2497673.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(9619..10644)
                     /locus_tag="PC407_002230"
     CDS             complement(9619..10644)
                     /locus_tag="PC407_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2497674.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDEYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10719..12287)
                     /locus_tag="PC407_002231"
     CDS             complement(10719..12287)
                     /locus_tag="PC407_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2497675.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHVLEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(12400..13311)
                     /locus_tag="PC407_002232"
     CDS             complement(12400..13311)
                     /locus_tag="PC407_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2497676.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13295..14281)
                     /locus_tag="PC407_002233"
     CDS             complement(13295..14281)
                     /locus_tag="PC407_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2497677.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLNEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14640..15041
                     /locus_tag="PC407_002234"
     CDS             14640..15041
                     /locus_tag="PC407_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2497678.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLVAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15044..15442
                     /locus_tag="PC407_002235"
     CDS             15044..15442
                     /locus_tag="PC407_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HDF2497679.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMVG"
BASE COUNT         4915 a         2720 c         1734 g         6245 t
ORIGIN      
        1 caatgaattt gtattatata ataagtattt ttaggattct gttacataat attatttaaa
       61 tattcttcta tatttctctt cttcttcttc agtaaacaca ttaaatacaa cttttaaatt
      121 tttttcagga ttttctttta gccatagtct aactctactt acagcaagat tagctgcctc
      181 tttttttgga taaccaaata cacctgtaga aattccacaa aaaacaatat tttttatttc
      241 atcaatttct tttattgtat ttaaacatga tttataacaa tgaagcagtt gcttttcttg
      301 ctctttactt ggctgaccac cagatacaat aggtccaaca gtatgtacta caaattttgc
      361 aggtaaacaa tagcctctag ttatcttagc atctccagta tattctaaat gcccttgttt
      421 ttttattatt ttgtcacaat cttctctaag tctaggacct gcgcatgaat gtatctcatt
      481 atccacgcat agatgtaatg gttgtaaaca ccctaataac ttgttatttg cagcatttac
      541 tattgcatct gctcttaaat tcgttatatt acctctccat atggcaatcc cttcctctac
      601 ttcttctata tcatttacat ctactaactc tctttcttca cattcaatca acaataaatt
      661 atgtaacata ttaagtatct ctggagcaat ttcttttggt ggatatacat tcaaaagtcc
      721 tctaagtata tctctaggct taatttcatc atttgataaa ttgtatattt tagaaggaac
      781 ttcttttgaa aaatatgcaa ttaaagtgtt tatatttttt cttctttgtt cgtcagttag
      841 aggcttgata gttttatcaa aatcctcaaa taaatttaca tcatcagcat aatctctcca
      901 cttcatagcc atctctccat tcttgtattt tagacttaaa aataattatg tatttatgta
      961 catatttatt tatttctata aacacctaca taaatcctgt taaaagtgta aattttttat
     1021 tatgcaactt ctctggttat ttctattgat tgatagtaaa ttattgtacc taaatttaat
     1081 ccaactaaca agtaactaaa cctactttcc taactgcaaa atacactatg tttgccactt
     1141 gtatctcatt tgtaaataaa taaagtccct acaaatacat taactaggct atgtactaaa
     1201 ctgtaggaac tttaaaaata tattattctt aattaagttt tggccaataa tttttatttg
     1261 cttctattaa atcatctagt aattcttttg caactgttgc actaggaact gtcttagata
     1321 gcataagtgc ttgccacaat ttattataag acttctcaat ccaagcatca actactaatt
     1381 tttcaactgc taattgttgt tctattaaac ctttttggaa tgttggtatt ttccctattg
     1441 ataatggttc atacccgtct tttccaacta tacatggaat ttcaaccata gcatcatctt
     1501 gtatattttc tatagctcca ttattttgaa ctatcaataa catacgctcc tgtgtattaa
     1561 aagctagagc tgtagctaaa tccactataa actctgcatg tattcctatc ttaagcccac
     1621 tgcccttaga atgtccttgc ttttctatca atgcacatga accaaaaact tctttttctc
     1681 ttccatcaat aacttcattg gcacgagtat aatttacatc agaatgattt acaacataat
     1741 ctggaaatag atagtacttt aagtaactac ttggtattgt agttaaatct gtttttacta
     1801 tttctttagt aaataaatta gtttcaaacc aactatcttt cttgtcattt ttcattcctt
     1861 cttcaccatt tataaaacca tattctttta tatgttctac taattttggt aaaagttcat
     1921 ttcctttttt atctttaatt gaagtatacc aaccaaagtg atttaatcca aagtatctta
     1981 tatccatatg tttacgagat ttgagtcctg caatccttgc tatattatgt tccataccta
     2041 ttggcatatc acaaatattt attaccctag aattaggtct caaccttcta acagcttccg
     2101 ctattattga tgctggatta gaataattta acatccaaca atttggagaa tacttttcca
     2161 tataatctat gttttcaaga acaccttcta tagacctcat tccataagct attccacctg
     2221 gtccacaagt ttcttgacca actactccat gttttagagg tatcttttca tctagttctc
     2281 tcatttcaag ttttccaact cttatatgag ctaaacaaaa atctacatct gtataagcct
     2341 ctttagggca tgttgttgct aaatactcta tattagggtc cttttccttt attagtattt
     2401 cacaagcttt agctacctta ttttgtcttt cttcatcatt atcatataat ttgattgctc
     2461 ttaatggtaa cctatctaag ttatctagca acattaatat gatttctggg gtatatgtac
     2521 ttcctcctcc agctattact actgaaaatt tttccataat aatatctcct tttctattta
     2581 aaaatttact gtatttataa ataaatttat aaatactagt actttaaagt aaaagggagt
     2641 tcatttatta aatctattga tttaataaac tatagccaaa cttcaagcac tatttacaag
     2701 aaatttaatt cttattaatt cttgggtcca tcatctcaaa tatatctaca agttcagata
     2761 ctaaagaatg agcaaataat gcatttgaat aatggtcttc agagtgcaca actaaaaaat
     2821 cagtcccact atcttcacta ttcatagcat ttgatagtaa ttctaaatga tgctttgatg
     2881 ctgtttgtat tttctctgag gctgaattta aaaggtgttt tgctttattg tagtcacctt
     2941 tttttatttc cttaagagac atcattacat cacttttagc atctcctgct tctgatatta
     3001 tactaaatac tttttcttcc atttcaggta ccatatcctt ctccttctaa tgacttgtta
     3061 aaaatcaaag ctatcatcat ctaatgttgt agctaattct gcatttttat ttaattcttc
     3121 cttctccatt atcttaaaga atggataata tatcaaggtc ataagtatta tacttgcaaa
     3181 tactaatacg attgccttcc aatctaatgt catcaaaaat gctgcaaccc ctggaggtaa
     3241 aaagcctggt ggttctgcaa taggtaatcc tactaatcct acttttaatg ccataaaatt
     3301 aagtgctcca accaatacag gtccaaatac aaatggtaca aacatatatg ggttcataac
     3361 tattggtgtt ccaaatatta ctggctcatt tattccaaat agtgatggta aaatcacaac
     3421 ttttcccata ctattaaaac tcttacactt acttcttgac ataaggtaca ctaatcccaa
     3481 agttatccct gaaccagtta cttgcaagaa accatagtaa aatcctattg taaacacata
     3541 ttttgggtct cctcctgctg ctatcagtgt tgcattttct gccatatatg tagctgctat
     3601 tggcatccat atacttgaag ttattgctgc tccatgaagg ccaaagaacc ataataattg
     3661 agttataaat agtattatag ttactgcaaa aacattatct acactgctta tagctggtgc
     3721 taagaaattc attataatag ctggtaatgg ttgacctgag acatttttag ttattgctgc
     3781 tattgctgta aataacatta tacatactat cattggagtt aaatttctaa aacttttatt
     3841 tactacacta ggtacttctt ttggaaatct aattacgaga tttttatcat tcataaactt
     3901 agtaatatca actactaaaa gtgctactaa tatacttaca aataaacctt gtgcatctaa
     3961 ataacttgta tctaatacac catctactac ttgtccacat acaacaagtt gtgcaacaac
     4021 tgctgtcata cctatttcta catgattaag attatatttt ttagctagtg cctgagctat
     4081 aaaaaatgct gaatatatag ataataaact catagtaaat ttattaggga taagtaatgt
     4141 atccatattt tcagataata atgtttttgc acttccttca ggtaacatat tgtttaatgc
     4201 cataaataac atacttaaag aaccaacaat tattattggg gtaatgctta tcataccttc
     4261 ttttatagct tgaagatgcc tttgtttttc aatcttttgt gctattggta gaagaaatct
     4321 tcttaaatta ttttgaagtg cttccatttt tattcccccc aattatctat ttttatataa
     4381 ttcgattgcc gacttcataa cagaaggagc atccatacga ccataatgct tagtatctat
     4441 taaaccaact gctattttgt aattcttaca agcttcaact acatcatctt ccttgaacct
     4501 aatttgtggt gctacaagta ctacatctgt atcattagct tcaatgtatg attttaaatt
     4561 ttcgaatgat gttgcctcta tatttatagt tttagttttt aacttttcat gctcactaca
     4621 aaaacttttc atcttttcca caagtacacc tgttgaagct ccatttgcac atactaataa
     4681 aatattcata attaccctcc tacaatatct aacctctttt attttctaat tttttatcag
     4741 ccaattctta ctctaattcc tatcagtttt tactttaatt cttattattt tattttaatt
     4801 cttattaata tttactctaa ttcttagtac tgccaatgat taaacttgaa agccgtatct
     4861 ataacttctt cttgcttata tttttctaat atttttaagt atctttcttt ataatcatta
     4921 tctacttctt tttctggtat tactatacta gcttcattta aaaagtggaa tgaatccctt
     4981 cccatttcta atcctctata tgtatgcttg tcaagtgcca cgtccataac ttctggaact
     5041 ttacccattg caaatccttt tataattata ttttttaata aatcacttga tctatctttc
     5101 tcacatgcac aaagatatct tattgcatgt atgaaaaata aaggtctgtc tccatctgca
     5161 tatgaatatt cctttcttac ttgatttaaa ttatttatga ttattacagc tgatgggtcc
     5221 cccattccta tatcttcaac tgatattgta agtatccttc tccatagctt atcttctaat
     5281 tctggtgatg atatatatag ttcataagca aattcacaag cttcttccac catatttctt
     5341 cttatagctt tttgaaatga cgatattatt tcgtctgcac taaatccgtt tcttgtagtc
     5401 attcttgccc atgggtcatt tgccttttta tctcttaata ttttatcttg catattattt
     5461 actccttttt atttaaattt taattattta tattactcat ttaataatat aagcaagtat
     5521 tatgccagtt tttttagaca ctttaaaaca ctgttatttc aatcattttt atcatcaaaa
     5581 cttagatact ttgtctaagt atatttatat gtgtctaata ttaatattat tttagactaa
     5641 aaaaaattac caaataagtt tttatactca ttggtaatct ttttcgatta tttctgcata
     5701 ttctaatatt aataatattt gatagcactc tttttcatta atttctatat tatatttttt
     5761 atctaattga gatactatat cttttatttt gcttaggctc ttgtgataat caagtgtttt
     5821 ttctttatca agcttttcaa tttctctagt atttccaatc aacccatcta ccatgaaagc
     5881 taaatgcatc ataagtccaa ttactttatc gtatttcttt tctatattaa atactaaaaa
     5941 caattcatca gacacatatt ttatatcttt aaatatccta ttataattag agctttttaa
     6001 ggatgtcttt aaaccttcaa ctacatcatc ataacctata cttatcttta taatattttt
     6061 taatttattg atattttcat ccttatctat atcttttttg ctaatatagt caattcctct
     6121 aatattaatt ctttgttcat tgattactgc aacaatatta ctactttctt taagttttgt
     6181 aatatttaaa cttaaatctt tttctgtttt atatgggaca gatataatat ctattccatc
     6241 taagtaccta ctcatctttt cttctaatat ttttcttgtt ttattatcta caccttctga
     6301 aacatgtgat gctataataa ttgttttaga cagattttct ttattttgaa tttcattagt
     6361 tccaaaataa tactttctcg tgttagtgat agagttgtat acgtcattta aaggtaatcc
     6421 catattggcc ttctgaactg cttctaacac agttaatgtc gtcaccatag gaattactct
     6481 tacttcacac tttttttctt tctttataat ttcatcaaag gagtttaatg aacccatatc
     6541 tgtaaatagt aatacatctt tattgtctat attattaatt aaatttttaa aatctacaat
     6601 aacttcatca tagcttttat ctaacttcat atcgaaagaa atagcactgc actttccaat
     6661 aatatcacca actgctttta ccattacttg ctgtagtttc tccatgcata gcaactataa
     6721 tattaatgtt attcactatt ttattttgtt catcttgttt agcttgtact atgaataatg
     6781 taatatatgc tacagtttct atagataaaa ataaatcaaa ttcattttca attattccaa
     6841 ctatttttag tgctgtttta aattcacttc tgttatttct tctaatttga ttcaaatcta
     6901 tgttgtttgt agagctactt ttatccaata cgctttttat ataaattgcc attgacttaa
     6961 atattgattc acttatatct atttcttcat cttctttaat aatttgtctt attgattcac
     7021 acatgctttt aaacttaaca ttattaaata gagaagcatc tgctatattc atttctacag
     7081 atttattaag attagtattt actgcattaa taatatttat atcattgtag ttaataactt
     7141 cttcttctcc taatgacgta aatacaaaat attctattcc atgtgttata acttcatctg
     7201 ctttaataga cttctctcta taatcatcac tcacttcatt tatagattta tcaaaatatt
     7261 ttttctctat ataaactggt tctttattat ctcttttata tcttaggtat ccatttgcaa
     7321 cagctaattt tatatcattt tgaagttgac ctacattatt tttacaatca taaagtaata
     7381 gtgatttaat tatttcttta tgtactttta ttgaaataga aatacttttt gattcttcct
     7441 tgaaaaaatt tttgactaat tccattcttt caatcattgt cctatctctc agtgatggta
     7501 tttctatttt cattggtatt cttctattga atgtatctaa tagtgttgat gtcggatttt
     7561 ctgtagtagc acatattatt aataccttag aatctctttc tttcgttgca tctcctagtc
     7621 ttttaaatgt tcccttatct ataaaagtaa acaacatttc ttgaccttct gctggtagtc
     7681 tatgaatttc atctaaaaat agtatccctc catttgcaat ttccaaaaga ccttttctgg
     7741 aagattctgc acctgtataa gctccttgtt ctactccaaa aatatgtccc ataagtaatt
     7801 gtgcattatt agcataatca gcacaattaa atgttacaaa aggagcttta ttttttctaa
     7861 ttccactatc tatagaataa tcgtacatat atctagcaat catactcttt cctactcctg
     7921 tttctccata gataatagta tgaagtccat ttgtaggata gagcattgcg accttgcatt
     7981 tttctattat aggtcttaaa ctatgttctt tacctattag tgtttcaaaa ctatcttttt
     8041 ttattttgtt ttctactttt gttccttttt ttatgccaag caactcactt tgtgtatcaa
     8101 gcaattcatt cttgttaagg ttaagatttg cagggatata atacacaggt cttcctttta
     8161 tcttcgtaac cataccctca ttgaataatt ggtttaaata tgtacttata ttgtttcttg
     8221 caattcctgt cttttctgat aattggttag ctgtaatccc tttttcattt tccctatatg
     8281 atgattctaa aactttcatt actttatctt ttttatatat cattacaagc tcccctacat
     8341 tataatattt tattaactaa aacccctata cacactttat tttttaataa actcttattc
     8401 tttaactaag gttataattt atcaaacttt ccttacttta aattatagaa aattattgta
     8461 gaaaaaataa gattattgct cattttttct acattactat ttttaatttt atagcacttt
     8521 cttaacaaca tactttactt gtttttaatt ttattatatt tgaatttatt atatttgaaa
     8581 agtagaaata aaaaacccta ttttggagta gttttaaaat ttctccaaaa atagggtttt
     8641 agtttgtatt tcaaataaat acctgttcaa gtatttttat actaaacaac ttttaaattt
     8701 tgttgatatt tatttatttc tgatttagaa gaatatacaa aatgtccatc tgaaacctca
     8761 atccaactta atgacttttc atttgaacaa tcatatccat tagaattata ctctattcta
     8821 tttcttgact tagcatatct agggtcagga acaggaattg cagaaagtag tgattttgta
     8881 tatggatgaa taggattatt atacaattca tttgaatctg ccaattctac taattttcca
     8941 ttatacataa ctcctatacg gtcactaata tgtctcacca tagataaatc atgagctata
     9001 aataaacaag ttaaattctt ttcttgttgc aattttttaa ataaatttac tatttgtgct
     9061 tgtatagaca catccaaaga agctataggc tcatcagcta ttataaattc tgggtctact
     9121 gaaagagctc gtgctatgcc aatacgctgt ctttgtccac ctgaaaaatc agatggatac
     9181 ttatttgcat aacttctatc aagtcctact agctttagca tctcatatac tttattcatt
     9241 ctatctgttt tatttttaca gataccttga atttttagtg gctctgaaat tatatctgca
     9301 attgtcatac gtggatttaa tgatgaagta gaatcttgaa aaataatctg catacttttg
     9361 tttacatctt tttttataaa tttgtacgtt tttttatctg atatgcaatt tcctttgtaa
     9421 attacttctc caccagtaga ttcatgtagt cttattatag ttttaccagt tgtagatttt
     9481 cctgaacccg actctcctac caatccaaat atctcaccct tatatatatc aaaacttata
     9541 tcatcaactg cttttacagt agtacttttg tccaaatgaa aatattgctt taagtttttt
     9601 atttccagta ttttttctct attcattaga aatcaccctt ccacaattta ctctaactgg
     9661 tacatctact tcaggtgcat ctgggtgcaa taaccaagtt gctgcactat gtgtatcatt
     9721 aattttaaac attggtggct ctttttcata atctatctta agtgcatttt tatttcttat
     9781 tgcaaaagca tctccttttg gtgggttcaa taaatttgga ggcattccag gtatattata
     9841 taaatattca tcctgtgaat ctaaagtagg taatgaaccc aacaaccccc aagtatatgg
     9901 atgtctaggg tcataaaata tatcctctac agttcctatt tctacaatct ttcctgcata
     9961 cataactgct attctatcag ctatagttgc aactactcct aaatcatgag ttataaatat
    10021 tattgacaaa cctattttat gttgtaaatc ctttattaaa tcaattattt gagcttgtat
    10081 agttacatct aaagcagttg taggttcatc agcaatcaat acatctggat tacaggcaag
    10141 tgctatagct attactatac gttgtctcat tccacctgaa aaatgatgtg gaaattgttt
    10201 aaatcttttc tcaggattat caattccaac caattcaata agctctatgg ctctcttttt
    10261 tgcttcactt ttacttatac cttgatgaat gattacagct tctgcaattt gctttcctat
    10321 tgatatagtt ggattgagag atgtcatagg gtcttgaaat atcattgata tattctttcc
    10381 tcttatatct tccatctctt tttcagactt cttaaccaaa tcacttgatt taagtaaaac
    10441 ctctccattt ttaatatatc cattgtatgg gagtatcctc atcaaactct tacacagtac
    10501 agatttccca caaccagatt ctccaactat agccaatgtc tcaccttttt ttagattaaa
    10561 agatacattt cttacagcct gaacttctcc ttcttctact ttaaaactta cagatagatt
    10621 attaacctcc aataaatgtt ccaaaaacca tcaaccccta tgctattcaa accttaggga
    10681 tatattttta tccctaaggt ttctaattaa tttcagtatt attcaattgt ccagtcagct
    10741 atgttccaga atatacctac accatgatga cctaaaactg tatcaggtgt taatcctttt
    10801 atgtttggtt ttccaacata tattgcatct atataagcaa taaatgtata aggcatatcc
    10861 ttagtcattt ctacttggaa ttgtttatat aactttaatt tttcatcttt atcttctgtt
    10921 tctcttgctt tttgaagtat tttatctata gtaggattag agtaagcact gtaatttgca
    10981 cctttatctg ttccaaatac tttatatgta tggtcatctg ggtcaaatgg acttccccaa
    11041 cctattaaat gagcatcttg attagcccaa tctgtttcag ttacaacaac agctttagca
    11101 tcaacaccaa tttcttttaa ctgttgtgca catattttag ccatatcaac tctaacttgg
    11161 tcactctcac ctgctgttat ctcgaaagct aacttagtac cttctttttc atagattcca
    11221 tcagaaccta atttccaacc tagcttttca atttcttgtt tagctttttc tggattatat
    11281 tcaaactttt ctatatcagg attattatat ggtcccattt gtaaaggtga atatgctggt
    11341 acaccatgtc ctaataatac actgtcaact attgcttttc tatctattgc atagcttaaa
    11401 gcatttggta atcctttagc ttttttatct ttaaagaatt tagaattaaa attgtataat
    11461 atacctctat agtcggctgt tttcattata tttactttaa aattcttttc gtctttttca
    11521 aagttagaca tatctttagg agttatttga gctaagtcta attctcctga ttttaattgc
    11581 attgccttag ccttatcatc tggaacaatc ttaaagacta ctttatctaa tcctggttct
    11641 tttacaaaat aatcactatt cttaacaagt gttatacttt gacctttgtc ccatttttca
    11701 agtttaaatg gtccagtacc tattggtttt tgattaaatt catcagtagc tatatcctta
    11761 ccttccaaaa catgttttgg taatactcct actgttaaat aatcaagcat tgctgtattt
    11821 ggagctttta aagtaatttt tatagtatta tcattaacca catcaatttt tgttatatct
    11881 tcataatttg atgctatctc agaagcatta tctggattca ttatagtttc tattgtaaac
    11941 ttaacatcat ttgccgtaaa tttctctcca tcatgccact taacatcatc tcttaaatta
    12001 aaagtatatg tatttgttgc ctcatcaaat ttccagtcct ttgctagaca aggaaccacc
    12061 ttattatttt catcatgagc tgttaatcca ttaaatatta atgaatttat ttctccatgt
    12121 tcatatagtg caggatttat acttgtatag tcattacttc cataaacaag tacctttcca
    12181 tctttaggcg tatctgcctt cttatctttg tctcctccac tagaacaacc tgccattaga
    12241 ctaagtatca ttactaaact taaaactttt aacttcttta atttcatgat gctccccctt
    12301 ttgcttttta taaaatattt ttcaaagctt tgaccccatt tatttaaata gatatttcaa
    12361 atacctcagt atttgcttat ttagtactca aatgctgatt tataaattac tagatttttt
    12421 attattattc tttcttatat aattacctat atttgttata cacaccaatg tggtaaccaa
    12481 aaatataccc ggaatcagta taatccacca tctatttgaa agtaatgctt cctctgacaa
    12541 agataacata cttccccaag aaacaatttc tattggtaaa ccaattccta aaaagctaag
    12601 tgtcgattca gtaccaatag ctgcgcctat gtttgttaca accatgaaca ttattgatgc
    12661 cacaaaattt ggaagtaaat gttgctttaa tacatagaag aatcctcctc ccatgctctt
    12721 agctgctagt atgtattcgc tatttcttat ttgtctaact tctgttctca ctatcttaga
    12781 tatattcatc caacttgtta tccctattac tatggacata gatactggat ttgaatttcc
    12841 aagtatagct tgtacaaata ttataattaa tattgaaggt atactaagta tgatttctgt
    12901 aaacctcatc attgcatcat ctacgagttc agatgctgaa ccacttatac taccataaac
    12961 aattcctata acagttgata ttacagtgga aaatagtcct ataaataaag atatttttcc
    13021 tccataccat atcatagaat atatatctct acccattgag tctgttccaa ataaaaaagt
    13081 tttatttgga gctaaattag aacttgctaa atccatgtat gttggttcat gtgtcattat
    13141 aaaagatgag aatacacttc caatgactat gattgataaa ataataattg atatatatgg
    13201 taattcttta atttttttag ataaggtttt ctttgtttct atatgttcat cttctgctac
    13261 taaaatataa ttttctccta caatttcaaa atcactatat tgtggcttca ttagtatcac
    13321 ctctatcata tttcattcta gggtctattt tattgtttat aatttgagct aacatattcc
    13381 caaatactac taaagcccct gttattaagc ataaaactaa taacatatta taatcatgat
    13441 actttgcact ttcaaaacaa agtgttccta acccaggata agaaaaaact ttttcaacta
    13501 cataagttcc tcctagtata tgaggtatcg aaatggccat tatactaata tatgatggca
    13561 ttatatttct caggcaatgc ttaaaaacaa tagttctatt atttaaacct tttgctttac
    13621 ataaaagcac ataatcttct cttatttcct ctagtaattt atttcttatc atatatgtgt
    13681 aataccatag atggcttaat ataagtactg taagaggtaa gattagatgt gatatcctac
    13741 ttaatatact ggactcttct cccattgaat aagcaccact acttggaagt atacttaagt
    13801 ttatactaaa tataagtatg agtactaagg caacccaaaa tgatggaata cagttggtta
    13861 ttgttcctaa tttgcatatg attctatcaa tgagcttatc ttcatgtaat gtacaaaata
    13921 ttcctaacaa taaggctagc acaaaggtaa gtatataccc tgaaccacct aatattatag
    13981 tgtttatata gacatcattt ataacagaag taacattttg tttatattta aatgaaattc
    14041 caaattcccc ttttgatgca tttgaaatcc attttatata ttgactgtat attggttcat
    14101 tcaaaccaag ttttttcata gcattttctt tttcttgtgt actcattctt tctactccat
    14161 ccccatagta agagattaat gggtctcctg gagcaagtct tgccatgtaa aaaacaataa
    14221 aagataataa aaacattact aaaataaact gcaacaactt tttcagtata tcctttaaca
    14281 aaccccaacc cccctttgtt gcaataaaaa aaccacataa aagcaaacgt aaagtaacgt
    14341 taaaaccttc gtggttccat tatcattaaa caaaattaaa ttttctatat ttatctatga
    14401 gttaatttta gcatcattta ttaactgtgt aaacaaactt tttaaaaatt gttaaaattt
    14461 catcaaatta ttttatttat acctaaaata aaatttttat gttctattct tcattatttt
    14521 ctataaaatt ataaaatttt aggttgacta tataagaaaa gtatattata ttagtttgta
    14581 tgcaaaccat ttaagaggtg attttattga aaagtaatta tgactgtttt agaatagcca
    14641 tgctcttaaa agaactttat tctaaaacta tgtacacagt agaagaaaat tttaaagaaa
    14701 atggattaac acaccaacag attatagtta taaaactggt agctcataat caagaactta
    14761 caatatcaca actatgtgat gaaatgtctt tagctaaagg tacggtttca ggcataatca
    14821 gcagattaga acagattgga tatatagaaa aattcaagaa atctaatgat aaaagaaata
    14881 catacgttaa gtttacaaca acaggatttg aatttgccac aaattttaaa attaaaatgc
    14941 aagaaagttt tgatgatatt tttaaaaatt gtgatgaaaa cgaattgagt gacttagtaa
    15001 aaaatcttag aaatatattg gcaaaagtaa aggagagata attatgaatt ataaattaat
    15061 acttgcaata gtatttataa catcagcttt aattttctac actataggtg tatttggaga
    15121 aagaaaggca aaaattttaa aaaagaaaca tgtaattata ttttggcttg ggtttatatt
    15181 tgatacttta ggaacattta caatgagtaa catagccaac agccatactt ttgaagtaaa
    15241 atctgcttta tcacaaaacc ttcatagtat tacaggtctt cttgcaattg tgcttatgct
    15301 ttttcatgca agttgggcta cttttgtcct atacaaagat gatgaggaaa agaaaaaatt
    15361 ctttcataag tttagtatag ttgtatggac tatatggtta gttccatact ttataggtat
    15421 gtttattggt atggttggat agtaaaataa aaagtgtgta agaaatactg taggtaaatt
    15481 tgtataagac aaatttatct acatccttct ttcacacttt tacatattta tctataatac
    15541 tccattattt ctttcatgtt tcttaatacc tatatagaat atttaattgt ttctatagta
    15601 ttttattatt ttct
//
LOCUS       DAMJVX010000082        15446 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMJVX010000082 DAMJVX010000000
VERSION     DAMJVX010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.086"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(47..781)
                     /locus_tag="PC407_002236"
     CDS             complement(47..781)
                     /locus_tag="PC407_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HDF2497680.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMIYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            907..1623
                     /locus_tag="PC407_002237"
     CDS             907..1623
                     /locus_tag="PC407_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2497681.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            1757..2449
                     /locus_tag="PC407_002238"
     CDS             1757..2449
                     /locus_tag="PC407_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2497682.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            2439..3620
                     /locus_tag="PC407_002239"
     CDS             2439..3620
                     /locus_tag="PC407_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF2497683.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKLLYFNSEI
                     SVINQLVYQLRDFRSLIGESLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKMEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            3902..4357
                     /locus_tag="PC407_002240"
     CDS             3902..4357
                     /locus_tag="PC407_002240"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDF2497684.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            4347..5072
                     /locus_tag="PC407_002241"
     CDS             4347..5072
                     /locus_tag="PC407_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HDF2497685.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLNQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            5044..7425
                     /locus_tag="PC407_002242"
     CDS             5044..7425
                     /locus_tag="PC407_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDF2497686.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDNAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSCGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(7630..8859)
                     /locus_tag="PC407_002243"
     CDS             complement(7630..8859)
                     /locus_tag="PC407_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2497687.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            9459..10373
                     /locus_tag="PC407_002244"
     CDS             9459..10373
                     /locus_tag="PC407_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497688.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGVKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            10397..11032
                     /locus_tag="PC407_002245"
     CDS             10397..11032
                     /locus_tag="PC407_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HDF2497689.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTAQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            11408..12124
                     /gene="pyrF"
                     /locus_tag="PC407_002246"
     CDS             11408..12124
                     /gene="pyrF"
                     /locus_tag="PC407_002246"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDF2497690.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVRAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGADFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            12150..13193
                     /gene="carA"
                     /locus_tag="PC407_002247"
     CDS             12150..13193
                     /gene="carA"
                     /locus_tag="PC407_002247"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2497691.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGEILTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIAPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            13246..>15446
                     /gene="carB"
                     /locus_tag="PC407_002248"
     CDS             13246..>15446
                     /gene="carB"
                     /locus_tag="PC407_002248"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HDF2497692.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYL"
BASE COUNT         6207 a         1713 c         2785 g         4741 t
ORIGIN      
        1 tttataatta atacaatctt gcttttttat ttaaaaattt taattattat ttaaaatttt
       61 ttatcttctc tttatcccat ataaacaatt tctcagaatt atttaaagcg ccaaatacat
      121 ttcttttaaa atcaggcatc tctttattta aattagcaat aagatttttt atgtactctc
      181 tcctagtatg accatttgca gggcagggat ttgtgataat aggatagtta gaatcttttg
      241 aaattttctt agtcatatac tcttctatgt aaatcatagg tcttataact gttaaatcct
      301 gtctatctag gtatgtcttt ggggaaaaac aatttactct accctcataa aacatagaca
      361 ttaaaaatgt ctcaattgca tcatctttat gatgaccaag agctacttta ttacatccaa
      421 gttttttagc attatcgttc aaagcacctc ttcttaaatt agcacataat gcacaaggat
      481 ttttttcttg tcttatatca aatacaattt cttttatgtc agtttgaaat tcataaaaag
      541 gaacatttat ttctttacac aatttatcta gtggagaatt atcaacacct ccagtattta
      601 atgttatggc tatcaattca aagttttgtg gagaaaatct ttgataactt ttaagtatat
      661 gaagtagagt taaactatct tttccaccag aaagtccaac cgcaatttta tcattttctt
      721 gtatcatatc aaaatcttgt atagccttac gagctttact tagaagtttt tgtatagaca
      781 ttttatcacc atcctttaaa aatattaaat ttaagattta tttaagattt acttataaaa
      841 tacttaagtc ttatactcta aaattaacat tatagacaaa cataacaaaa aggagaagat
      901 ttaattatga aaaaatttaa attaatagga gccattattt tagtagtatt tataagtgta
      961 tttatatcta aagatagatt acttaagaaa gataaagatc caatagtagc tgctcaacat
     1021 gattcagaac aagatgttga ggcaaattct caaaataaaa atcactcaaa tagtatatca
     1081 aatatattat tggtaaacaa gacaaatgga attagcaaaa actatactcc tgaaaatatt
     1141 actaaggtaa acataccttt tgttgaagaa gcaacagagg aagaaaaaca aatggcagga
     1201 gaacctgcaa aggcagttga agatttagtt aagcaagcta attcagaagg aatacagttt
     1261 ttagggtctt cagcatacag atcttatgat actcaactgg atacttatac aagaagggta
     1321 aaatctcagg gacgagaaaa ggcagatgca tatgtagcaa agcctggtta cagtgagcac
     1381 caaactggac tttgtattga tttaacaaat ccagaaagat ggtttgttgg aagtacaaaa
     1441 gaagctaaat ggttggcaga aaatgctcac aagtttggat ttataataag atatcctgaa
     1501 ggtaaagaag atataacagg tacagcatat gaaccatggc atattagata tgttggtaag
     1561 gatgctgccg aggaaattta ttcaaaagga ttaactttag aagaatattt acaaaataaa
     1621 taatataaat gtgtaaataa tttgtattat attggaaatt tagaaatgaa taaatttata
     1681 gattacacta actaaaatat agtataattt tatatgaata aagctatata caaagattaa
     1741 gttaggatgt gtaaaaatga ataatacagt tcttgttgtg gatgatgaaa aggaaataag
     1801 agaccttata gagatatatc ttttaaatag tggatataat gtaattaaag ctgaagatgg
     1861 aaaagaggct ctagatattt taaaaaagga aaatattcat ttaatgatat tggatataat
     1921 gatgcctaaa atggatggga tagaagtttg tagaaaagtt agagaaagtt tgaatatacc
     1981 aatacttatg ttaagtgcaa aatcagaaga tatggataaa atacaaggga taatgactgg
     2041 tgcagatgat tatttgacaa aaccatttaa cccacttgaa cttactgtga gagtgaaatc
     2101 actgattaga agagcatatt attttagtgg tgctaataat gaagataaag atgtcataaa
     2161 ggttggagta gtaattatag ataaggaaaa acatagtgta acagtggctg gaaatgaaat
     2221 aattttgaca tcaagagaat ttgatatatt atatttacta gcaaataata aaggcagagt
     2281 atatagtaca gaagaaatat ttgaaaaggt atggaaagag aaatattacc aatctaataa
     2341 tactgtaatg gttcatatga gcagaataag ggataaaatt gaaaaatata cagatggaga
     2401 aaaaataatt catactgtat ggggagtagg atataaaatt gaaaaataaa agtttattaa
     2461 atcatatgtt tcgacccata ttagatctta taataagctg tgctttaact tcaatgctta
     2521 ttttaacaag tatgtatata ataaaattat tatatttcaa tagtgagata tctgtaatta
     2581 atcaacttgt atatcagtta agagatttca gaagtcttat aggagagagt ttattttatc
     2641 catttttatt tacaatcaca tcattagcaa tttattccat gatatcgtac aagagaaata
     2701 agcaaattgt agattttata agaaagacta atagaaaaaa tcaaaaatta gaaatcatta
     2761 aaagtgctgt aaatttaatg gatgaaggta acttagaaaa aagtatagat attgagtatg
     2821 atttagatat attaaaggat aaagaaaagg atgatattga tgaactagca cataaaatca
     2881 ataatatagt ccatcaactt agaaatataa ctattgaaga gagacaagct cagcagacaa
     2941 aaacagattt aataacaaat gtgtcacatg acttaagaac acctttgaca tccataatgg
     3001 gttatttagg agtgatagaa gaaggtaagt ataaagatga agttcagatg atgtattatg
     3061 ttgatattgc atatgaaaaa tcgaaaaatt taaatgttct tataaatgat ttgttcgaat
     3121 tgactaaaat gcaaaataat acaataaaac taaacaaaat agaaataaat ttagtagagt
     3181 tattaagtca agtagtatct caatttgaaa tttattttaa acaagaattt atggttagta
     3241 gaattaattt taatgaagaa aaattaattg tgaaagctga tccaaataaa ttggtaagag
     3301 catttgaaaa tcttataact aatgctgtga aatatggaaa agatgggcat tatgtagata
     3361 tagtcacaga gaaaatggaa gatatggcgg ttgtaaaagt gataaattat ggagaaccaa
     3421 ttccagtgtt agatttacca aatatatttg atagatttta tagagttgaa aaatcgagaa
     3481 atagaaatgt tggaggttct ggattgggtc ttgcgattac aaaaaatatt gtaaatttac
     3541 atgatggaga aattactgtt tcaagcgata aatatcaaac tgtgtttgag gtacaactgc
     3601 caataataca atcagcttag ttataaaata aaagtacgat tatgtgatat aaatattata
     3661 acaataaagt gtttacaaaa ataaaaaagt gatatataat ttaagtcagt ttaataagat
     3721 acttatcaag aattagggga gagagttagc tcagtgatcc tataccaacc ttttatcagg
     3781 tggtcctgct aacaacgatg agatgacttg aaataatata ctcttcttat caataataga
     3841 taggaagagt tttttttaga attaatgtaa caaactttaa agtataatgg aggattaaat
     3901 aatggaaaaa gtagaaagtt ttaagctaga ccatacaaaa gtaaaagctc cttttgtaag
     3961 aaagtgtagt gtacttgatg gagtaaaggg agataaagta actaaatttg acttgagatt
     4021 tttacagcca aatgtagaaa gttttggaac tgctgctatg cacggtttag agcatctttt
     4081 agctacttat ttaagagata cattagatgg tgtaatagac ttatctccaa tgggatgtag
     4141 aacaggattt tatttaatac tatggggaga tgtagatgcc aaaacagtta agattggttt
     4201 agaagaagca ttaaaaaaag ttcttgaatc agataaaatg ccagcagcta cagccataga
     4261 atgtggaaac tacagagact tatctttatt tggggcaaag gaatatgcaa aagatgtttt
     4321 agataaagga ttttctttaa atatatatgg agaataattt aattggaaga gaagatataa
     4381 gcataaatca aagtgaagtt ttaagatatt tacaatacag aggtcaagag atagataaag
     4441 accttaaata tactattgat gaatgtgtag ctataactaa aaataaaata aatcctagat
     4501 atttatctag gatttatcct attagaataa ataaagataa tgttgtagaa ttagagggaa
     4561 caaatttaac attagagagt aaagatgtat atgaactttt aaaagaatgt aatgagtgtg
     4621 taattatggc agctactata gggatagata tagaaaaaga aataagacga cactcttatt
     4681 caaatttaac aaaaggacta ataattgatt catgtggaac aacagcaata gaagaagtgt
     4741 gtgacattgt tcaaaataat ttagaaaagg tttttttaaa ccaagataaa tttataacta
     4801 tgcgatatag cccaggatat ggagatttac caatagaaaa aaatatagat atattaaacg
     4861 ttttgaatgc tcaaaaacaa ataggtctaa caattacttc taatggaatt atgataccga
     4921 gaaagtcagt agtggctatt ataggtattt ctaatacaag aataaataaa tctaagaaag
     4981 aaaagtcgtg tgaaaattgt aaaaactata gaaactgtac atatagaaaa ggagcgaact
     5041 cttgtggaaa taagacaata tctgaaaaat aatatattaa tatttgatgg tgcaatggga
     5101 acaatgcttc aacaaaaagg gttaaagtta ggtgaaaatc cagaagtatt tggtcttcaa
     5161 aatccagata aacttataga aatacataca gcatacttag aggcaggttc gaatgtaatt
     5221 cttacaaata cttttggatg taatgaacta aaattagata gtaagtatac tgtagaagag
     5281 gtgattgaca atgctgtttt ggtggctaga aaagctatag aaaatgtaga taatactaaa
     5341 ccaagatatg tagctttaga tattggtcct ataggagaaa tgctagagcc tatgggtact
     5401 ttaagttttg ataatgcata tgagatattt aaaagacaag tactacaagg cgttaaatct
     5461 ggagttgatg taatagtaat agagactatg atggatttat atgaagctaa agtagctgta
     5521 ttagcagcaa aagaaaactc tgatttacct attttctgta ctatgacatt tgatgaaggt
     5581 ggaagaagtt ttactggatg tatgccagaa tgtatggtgg ctactataga aggtctaggt
     5641 gtagatgcaa taggagttaa ttgttcatta ggacctaaac aacttttacc aatagttgaa
     5701 aaaatagcat caagagctac agtccctgtt atggttcaag caaatgcagg tcttccaaat
     5761 atagttgatg gagaggctat atatgatgtt gatgcaaaag aattttttga aggggttaaa
     5821 aagttcgttg aagttggtgc tactataata ggtggatgtt gtggtacgaa tccaagtttt
     5881 ataaaagaaa tatctgagaa tataaattct gttacaaagg gatgtatcga gaaaattgat
     5941 aaatgtgtag tctgttcacc atcaaaattt gtggaagtac aatcgcctac agttgttgga
     6001 gaaagattga atcccacagg tagaaaatct ctacaagaag cattaaaaaa tgaaaatgtt
     6061 gattatgcta taaatttagg cttagaacaa gtaaatgctg gagcacaaat acttggagta
     6121 aatgttggtc ttccagagat tgatgaaaaa aaactaatgc caaaattaat aagagagatt
     6181 caagcagtag tagatacacc tttacaagtt gattcttcca atgtagaggc cttagagcaa
     6241 ggacttagat attataatgg tagaacaata gtaaactctg taaatggcaa agaggagtct
     6301 ttagaaagta tattaccaat agttaaaaag tatggttcat gtgtggttgg acttacactt
     6361 gatgaaaagg ggatacctaa aaaggctgaa gaaagatttg aaatagcaaa aaggatagtc
     6421 aatagagcag tttcttgtgg gataaaacca aaagatatat ttatagactg tttatcatta
     6481 actgtttcag cacaacaaga agaagcaata gaaactataa aagctataac aatggtaaag
     6541 acactaggag taaaaactat attaggtgtt tcaaatattt catttggtct tccaaatagg
     6601 aaagcactta atgcttcttt tctaacatta gctttagggg cagggcttga tttagcaatt
     6661 ataaatccaa atgaatatag tatgatggaa gctatcaatt cttttaaaat attaaataac
     6721 acagataaag gatgtataaa ctatataaat caatatagta atataaataa ttcaaaaaaa
     6781 tcggattcat ctacaaagat tgacaaagat ttgccattgg atattttagt tgaaagagga
     6841 ttaaaagatg aagcaaaaaa tgtaacactg aatcttttaa aagagaagga tgaaaattac
     6901 atattagatg agattttaat tcctgctctt gataaagtag gaaaaagata tgatagcgga
     6961 gatatatttt taccacaatt gattcaatca gcagaaacgg taaaagtttc attaaataca
     7021 ataaaggaaa ctttattaag taaaagtagc aataatgtat ccaaaggaaa aataatagtt
     7081 gctacagtaa aaggtgatat ccatgatatt gggaaaaata tagtaaaaat aatgctagaa
     7141 aattatggat atgaggttat agatttaggt aaagatgtcc ctattgaaga agtagtggaa
     7201 gttgctaaaa agagaaatat aaaattagta ggattaagtg cacttatgac tacgactgtt
     7261 caaagtatga aagatacaat tcaggcactg agagataatg agataaatgc aaaggtattt
     7321 gtaggaggtg cagttttgac agaagaatac gctgaagaaa tgggcgctga ttattattca
     7381 aaggatgcaa agtctgctgt tgaaatagca aagttaaatt tttagcatat aaattaaaat
     7441 aaaggtttta ctaaatagga tattttatat ataatataaa tataagtttt agatacttat
     7501 gtttaaaaca ataggaatta atctataata atgagattaa ttaatataaa aaatatttat
     7561 catcaacatt aaaaagggct gggaagtctg gtataacttc cattagccct ttttagttaa
     7621 aatcatatgt taaaaagccc aattaccatt tttaaaaatc tgtatttgtt ttccatcata
     7681 agtctcacca ataatatcca tatcagcaga accaatcata aaatctacat gtgttaaact
     7741 gtcatttaca ccatgttcat ctaattcttc atcttttaaa ttgtttccac cttccagaca
     7801 agtcttataa gcagaaccaa ttgcaaagtg gcaagaagca ttttcatcat atagagtatt
     7861 ataaaaaatt atatccgtat tggatattgg tgagttgtat ggaacaagag caacttctcc
     7921 taaataatga gagccttcat ccgtttcaat aagtttttct agtgtatcta atcccttttc
     7981 agcagagaaa tctattattt taccatcttt aaatgttaag aagaaattgt caattatgtt
     8041 tccaccataa acaagaggtt ttgtactata aacagtccca tttactttaa acttatgtgg
     8101 cattccaaaa atctcttcag ttggaatatt cggattaaaa cttattccat tagggtcttt
     8161 ggatgctcca cttagccaaa catgaccctt tggcaagtct agagtcaaat cagtttttga
     8221 ggatttatat tttaaagttt taaagttatt tttatttaga aagtccattt tagatttaag
     8281 attttcatta tgttcttccc atgcttttat agggtcctga ccatctactc ttgaacattt
     8341 aaatatagca tcccacagtt tttctattgc tttatcttct gataaatttg gaaacacttt
     8401 ttttgcccaa tcttttgttg gcacagagac tatactccat ttacatttat cagttagagt
     8461 gtaagaacgc cattctttta aagcttttcc agatgccttc tgaaaatttg caactctttc
     8521 agggtcaaca tcttttaata aatctggatt ttgggcatag actgataaaa atgccccacc
     8581 ttcttttgct atatctacat attgttctgc tgtccatttg gggaactcat gaaatgcttc
     8641 atctggagca tacaagtatt taataagagt actttcttca tcagaccact ctatataaac
     8701 atttttagca ccacatttat atgcttcttt aagtgcattt cttgcaaaat cagcacattc
     8761 tattggtgac tttataagta gaatttgacc tggctgtatg ttaactccaa cttttattgc
     8821 taattcagcg tatttttcta aatttctatt taaattcata tttatcctcc tatattttta
     8881 atattgaata acttataaag tattataaca ttacttatgt ttttaatatt gaacaactta
     8941 taaaatatta taactttact tagattttgg gtcatattca aaatatttat actttataga
     9001 ttgagaaata aaaaaaatat tttaattaaa acagattatg aggataacaa acttatttaa
     9061 gcagtatgtt ttttgttgat aataatatta aagtagaaat aaaaatactt ttttagtgaa
     9121 aagctataca agaataccga aaaaaactta taatataata atgagataga tatttataaa
     9181 tgaaaaaaat ttttctggca aagcttaatt aaaaaggtaa tagctagtgt tgttatttaa
     9241 attagcatag aaaatcaaat aaaaaaatta tgctaatgta tagacattga taaggtagaa
     9301 tttaagcctt ttgaatgcta tattaaacta gagtagatat tcttatgaat ttcaaaatag
     9361 ggcaggagga aaaaggaaat aaaactataa aaaatataaa gtatttatat aaatttatag
     9421 agtttatacg tcagaagatt tcagttagga gggcataaat ggagctttat gaagccattt
     9481 tttatagaaa atctgtaaga aatttttcca gtaagaagat aaaaaagcca ttgatggaag
     9541 aaataaaaag aagttgtaaa aatttagaat acttaaatga agatttaaat attaaagcac
     9601 atgttgttga tagaggtcat ataatacatt ttttgatggg taaggaatgt aaagtaaagg
     9661 cacctcatta tatagtagtt acatcagaca aaggtgagga ctatttacag aatataggat
     9721 ttgctacaga aggtgtagtt ttgcgcctta caacattggg aatagctact tgctggttgg
     9781 aaggaaactt aaagagagaa gatatcttgg aatttgtaga tattgggaat aagaattttg
     9841 aagaagatga ggaagatgat ttagattatc tagctggagt aaaaacagaa gaagatgatg
     9901 aaaacaaaat agaaaatcct tatataataa tagcatttgg atatccagca aaaaatgaag
     9961 agttgtttag gacgagatat gctagacctg atagaaagcc agtaaaagat atgataaata
    10021 agataaatag gaatagaaag tggaaagata tcttagaatt gacaagaaga gctccatctg
    10081 taaaaaattc tcaaccgtgg tatttccata aggatgaacg aggtcttcat ttatttgaaa
    10141 agagacctaa gaaacactgt gaagatatga ataaggttag cttaggtgta gccttaagac
    10201 attttgatat agcttgtata aaaaataaaa tagatgtttc atatgaaaaa cttcctataa
    10261 ggaataaaat aggaaaatca tattttataa ctgtagtcga gcatgtgaaa ccagaagaag
    10321 aaacccaaga agaaaatgta actctagaaa aagaagaatc acaagatgaa taaaattttg
    10381 gaaggggata ttggatatgt ataagtgtgg aaaatgtaat cttagaggct gttctaatgg
    10441 aaatatggat aaattaatga agatatgtcc aactagagaa attgaactac aacaaaaagc
    10501 aaagcaactt tatgaagaag aagagaatag aaaaatagct tataatgctg gattagttga
    10561 agcagagggg tattgtcaat atactagact aaaagagatt atagagtttg ctaaaaagtg
    10621 tggttataaa aagattggca tagcattttg tattggacta aaaagagaaa tgcaaacagc
    10681 acaaaaaata tttgaacatc atgggtttga agtattttct gttatatgta aaaatggggc
    10741 aatgcctaag agtacaattg gactagaagc atctcaaaca atatcaaagt gttcagatga
    10801 ggtaatgtgc aatccaatag gacaagcttt atttttaaat gaagctaagg ttgaccttaa
    10861 tgtaattctt ggattatgtg ttggtcacga tactttagcc atgaaatata tggaagctcc
    10921 tataacaact ttagttgtca aagatagagt aactgggcat aatccagttg ctgcaatata
    10981 taatgcagaa tcatattttg agaaaaaatt gtttgataaa gatgaagaat aattcaaaga
    11041 cttattgaca taaaaagcaa ttagtggttt aattataaaa aagataaaac tttaaattat
    11101 cctatagata cataaagtta tataaataaa gaaaaatatt acagctaaat tccttaaagt
    11161 agtccagaga gactaaaggg ttatagataa tgttagtata taacctctag taaatcttct
    11221 agaggttttt gatgagtata aaataaaaat ttaaatataa taaagtcttt taaactagtc
    11281 cagagagact gggaaagata tctcatagct aatgaatgga gttatgtata gataaaatcc
    11341 ttatgcctct tttggtgtga ggattttttt atgattattt aggctttatt aaaaaggaga
    11401 tgtaaaaatg ataaatggta aagaaaaatt gatagttgca attgatactg acgaatttga
    11461 taaagctaaa gaactaatag acagattaga agatagtgta gacatattta aggtaggtct
    11521 agagcaatac gttgccacaa aaggtaaaac aatagattat cttaaagaaa aagggaaaaa
    11581 aatattttta gaccttaaat tccatgatat accaaatact atgaaaagtg cagtgagagc
    11641 tgcagtgaga gataatgttt ggttaatgac aatacatgta tcagatatgg aaggaatgag
    11701 acaatgtgca ctaattgcta gggaagaagc agaaagatta aatatagaaa agccattagt
    11761 agttggagta acagttttaa cttcattaag taaccaagat ttacaggaca taggatgtaa
    11821 tatgacaaca gaagagttag ctataaagag agctaaactg gcaaaagaat caggcataga
    11881 tggggttgtt tgttcagcgc aagaagtaga taaaatagta gaagcatgtg gagctgattt
    11941 tataacagtt tgcccaggaa taagacctaa atcagcagag gttggagacc aaaaaagagt
    12001 tgtaactcca tcagatgcaa taaaaaaagg tgctcattat ttagttgttg gaagacctat
    12061 aacaaaagca gaaaatccaa gagaatcagc tattaatatt gtaagagaaa tagaaaatgc
    12121 ttagataaaa tatttgggag gtagcttata tgaaggggaa attaatttta gaagatggaa
    12181 cagtatttga aggaaaagca tttggatatc taaaggaagc tattggagaa gttgtattta
    12241 atacttcaat ggctgggtat ggagaaatat tgacagacgc aacttattat ggacaaatag
    12301 ttactatgac ttatccactt ataggaaact atggaataaa tttagaagat gttgaatcaa
    12361 ataaggtgca agtaaaagga cttattgtaa gagaaaaaag tgataaccca aataatttta
    12421 gatgtgaaat ggatatagac acatatctaa agcaaaataa ggtaatagga cttgaaggaa
    12481 tagatacaag agcattaact aaaattatta gaaacaatgg aactaagaga ggaattataa
    12541 ctttagaaaa tgtatcatta caagatgttc agtctaaatt agaagagcat tgtaacaaag
    12601 attcagtaaa aaatgttaca agaaaagaaa ttgaaaatat aaaaggtact ggagctaaag
    12661 ttgtagttat agactttggt gttaaaaata gtgtactaga gtctcttaaa gcttgtaact
    12721 gtgatataac tatagcacca gcaatgacta cagtagaaga aattttaaat ataaatcctg
    12781 acttaatctt attatctaat ggaccaggaa atccagagga tataaatgat atagttgaaa
    12841 atataaaaaa attaataggc aaaaagccat tgacaggaat aggtttagga catcaaattt
    12901 tagcattggc attaggagga aagacttcaa agcttgtatt tggacataga ggaggtaatc
    12961 acccagttaa atgtttagaa actggaaagg tatttataac ttctcaaaat catggatata
    13021 atgtttcaga aatgccagaa aatacagagg taactcacat aaatttaaat gacaatacta
    13081 tagaaggaat gagacataag gaattaccaa tatatagtgt tcagtacatt ccagaataca
    13141 caacaaaaga agatttagat tgtatattta ataaattctt agcattaata tagatttagt
    13201 attatttaca gatagtaaaa accatttaga taaggagaaa aatttatgcc taaattagat
    13261 agtataaaga aaactttagt attagggtca ggaccaataa taataggtca agctgcagag
    13321 tttgactatt caggaacaca agcttgccag tctttgaaag aggaagggat tgaagttgta
    13381 ttaataaata gtaatccagc cactataatg actgataaag aagtggcaga taaaatatat
    13441 atagaaccat taacaataga atttatagaa aaaataatag aaaaagaaag accagacagt
    13501 ttacttgcag gtatgggtgg acaaacaggg ctaaacctag cagttgaact ttatgaaaaa
    13561 ggcatactag ataaatatgg agtaaagata ataggtactt cagtagaatc tatcaaaaaa
    13621 ggtgaagata gagatatatt tagagaagta atgaaagaaa taaatcagcc tgtaatagtt
    13681 agtgatatag ttacagattt acaagcaggt cttgattatg cacttacaat tggttatcca
    13741 gtagttgtaa gaccagcata cacactaggt ggaactggtg gtggtatagc tgataatgag
    13801 gaagaattaa gagaaatact ttcacatggt ctacaattaa gtcctgttgg acaagtatta
    13861 attgaaaaga gtataaaagg ttggaaagaa atagaatatg aggtaataag agattcaaag
    13921 ggaaattgta tagttgtatg taacatggaa aatatagacc cagtcggagt tcatacagga
    13981 gacagtatag ttgttgctcc aactcaaact ttaagtaaca aagaatgtgc aatgcttaaa
    14041 aaagcatctt tagatatatt aaatgcagta gaagttcaag gtggttgtaa cgttcaattt
    14101 gcacttaatc cacatagttt tgaatatgca gttatagaga taaatccaag agttagtaga
    14161 tcatcagctt tagcatcaaa agctacagga tatccaatag ctaaagtttc atcaaaaata
    14221 gctttaggtt atactttaga tgagatagaa aatgcagtaa ctaaaaaaac ttatgcttgt
    14281 tttgaaccaa ctatagatta tgtggtggca aaaataccaa aatggccttt tgataaattt
    14341 aaaaaagcaa atagaaaatt aggaactaag atgatggcaa ctggtgaaat tatgagtata
    14401 ggaagtaact ttgaggctgc tattcttaaa gggataagat cacttgaaac aggaaaatat
    14461 tcattagttc acacaccatc tgaggagaga agcattgagg aattaaagaa aagagttgta
    14521 gtacctgatg atgagagact atttgactta gctgaaatga taagaagagg ctataaagta
    14581 gagatgatag aacaaattac aggtatagat aaatggttta taaataaatt taaatggata
    14641 gtagagcaag aagaaaaatt aaaagttatg aaaattgaag atttaactaa agactatctt
    14701 cttgaattaa agaaaaaggg attctctgat aaaggtatat ctgacttaat gaagataagt
    14761 ccagaaaaat tatatgaatt gagaagtcta tacaatatca aaccagcata taaaatggtt
    14821 gatacttgtg caggtgagat agatgctata tctccttact attattcaac ttatgagcaa
    14881 tatgatgagg tagttgttag tgataagaaa aaggtgatag tattaggttc tggtccaata
    14941 agaataggtc aaggtataga atttgactat tgttcagttc attgtgtaaa atctttaaga
    15001 aaaatggaca tagaaactat aatcataaac aataaccctg aaactgtaag tacagacttt
    15061 gatacatcag ataagctgta ttttgaacca ttaacagaag aagaagtttt aagtataata
    15121 gaaaaagaaa agccagaagg tgtaatacta cagtttggtg ggcaaacagc tataaaattg
    15181 gctaagtttt tacatgaaaa aaatatacct atactgggaa cagattttga tgatatagat
    15241 gcagcagaag atagagaaaa atttgatgat ttactagaga gactagatat aaatagacca
    15301 aaaggaaagg gtgtatggtc acttaacgaa ggtattgaag ttgctaatga attaggttat
    15361 ccagtattag ttagaccatc ttatgtactt ggtggtcaag gtatggaaat aacttataac
    15421 gaagaaaaat tatctcaata tctaca
//
LOCUS       DAMJVX010000083        15078 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMJVX010000083 DAMJVX010000000
VERSION     DAMJVX010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15078
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.087"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(30..1010)
                     /locus_tag="PC407_002249"
     CDS             complement(30..1010)
                     /locus_tag="PC407_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2497693.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            complement(1067..2269)
                     /locus_tag="PC407_002250"
     CDS             complement(1067..2269)
                     /locus_tag="PC407_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HDF2497694.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            2464..3018
                     /locus_tag="PC407_002251"
     CDS             2464..3018
                     /locus_tag="PC407_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2497695.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            complement(3133..3924)
                     /locus_tag="PC407_002252"
     CDS             complement(3133..3924)
                     /locus_tag="PC407_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDF2497696.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKPDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDFSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFKGMSGTEEILSNILDENCLLNI"
     gene            complement(3952..5040)
                     /locus_tag="PC407_002253"
     CDS             complement(3952..5040)
                     /locus_tag="PC407_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HDF2497697.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKHFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            complement(5094..6332)
                     /locus_tag="PC407_002254"
     CDS             complement(5094..6332)
                     /locus_tag="PC407_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HDF2497698.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGVRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELNCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKKHYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            complement(6310..7386)
                     /locus_tag="PC407_002255"
     CDS             complement(6310..7386)
                     /locus_tag="PC407_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HDF2497699.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHSITGILIPKANSELIENCIEALYSMNLQDMYVI
                     PIIETREFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            complement(7450..8586)
                     /locus_tag="PC407_002256"
     CDS             complement(7450..8586)
                     /locus_tag="PC407_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2497700.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            complement(8587..10149)
                     /locus_tag="PC407_002257"
     CDS             complement(8587..10149)
                     /locus_tag="PC407_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HDF2497701.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGVIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDIHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            complement(10236..12995)
                     /locus_tag="PC407_002258"
     CDS             complement(10236..12995)
                     /locus_tag="PC407_002258"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2497702.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            complement(12985..13614)
                     /locus_tag="PC407_002259"
     CDS             complement(12985..13614)
                     /locus_tag="PC407_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2497703.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            complement(13700..14278)
                     /locus_tag="PC407_002260"
     CDS             complement(13700..14278)
                     /locus_tag="PC407_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2497704.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLRHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            complement(14317..14895)
                     /locus_tag="PC407_002261"
     CDS             complement(14317..14895)
                     /locus_tag="PC407_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2497705.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
BASE COUNT         4839 a         2743 c         1559 g         5937 t
ORIGIN      
        1 tattttataa atttaagttc tttattatat tattttagta tatctttaaa gagcctataa
       61 aaattaccac tagagattaa ctctatatct ttctttttat aacctctatt ttcaagctct
      121 actactatgt tctgtgctgt atgtgtgctt tcaagaccat ctacacttgg cacactagca
      181 tttccagtaa aagaacctat agtctctttc cctaagtaat cactaaaatc aaaaccaaaa
      241 gatacatgtt ctatacctat aagatttacc atatagtcta tatggtcaac caaatgtatt
      301 aaatcttgct tcttaggatt actattagta aatcctctaa aactatttat tccaactaat
      361 ccacctgtat ttgaaatttc ttttaattga ttatctgtaa gatttctttt agcatcacat
      421 aaactcctag cattagaatg agaagctata actggcttac ttgcaacaga cattaaatcc
      481 caaaatgatt tttcatttaa atgtgacaca tctactatca tgcccaattt ttccatttta
      541 ttgactgctt ttttacctat ttcagttaaa ccacgatttt catcccctaa acatcctgta
      601 gcaagttcat tctgttcatt ccaagttaat gatgcatgtc ttgcaccaac ttctttataa
      661 aaataatcta gtaaatctat atttcttcca atatggctta atccttcaag accaattaaa
      721 atatttattt tgttttcact tataccttta tctatatctg aatatttttt tactatgttt
      781 acaacatctt tcatatcttc aatttcatct tttatagagc ttataatttg tttaactctt
      841 ttttcaggct ctttatcata tggtgggtct atccatatta caaaaatagc tccttctata
      901 tttgctcttt taaaattatc aagatggatt cttctaaata catctttttc cccgttaagc
      961 cttcttattg ttacatctgc ccaagtatct gaatgtccat cgaaaatcat aacaacctcc
     1021 aattaaataa aaatctttct aaaaaacaac ttcttttcac gtaaaattat atcaaaatat
     1081 ttcctactaa aagaccaaaa taattaccaa ctatgtatcc aaatgttcca actataagta
     1141 ttggccctac tagcttagac catccttttg atattgccat agctgctgct gttgtaggac
     1201 caccaatatt tgcattagat gcaagaatag cttcttctaa actaaactta aatatttttg
     1261 caactataaa tgtaactagc atgtttatta gtaccattat tccgcagtat aaaagtagta
     1321 atggtgattt ttgaattatt aatgttattg aagcaggaac acctactaca acaaagaata
     1381 tatatattaa aaatgtaccc aattcctgtg ctccttttat atcaccaaag aaattaggca
     1441 taaatgtggc aaacaacata gtcaaagtag ttattattag atattgattt ccaaataaag
     1501 tatttaacat tgctaaaaaa ggatttgata atggtattat agctttaaac caagttgata
     1561 gttctacaga tactgcgaca attataaagg cgctaccaat ggctattgct atatctttta
     1621 aagatatttc ttttcttccc caataatttg ctgctactgt ttctccttca gttataccta
     1681 tactttccat ttcatctaca tgtggatgtt taaaatgttt tctaaaaaat ccaattgatg
     1741 gtatagctat taagacaaag aaatataatg ccattaaaag attatctgct acaactgttg
     1801 ctgatactaa ttctcctggc gcatcaaacg cccctgacat agcagcaaag tttacatttc
     1861 cacctatata agaacctgtc atcatagctg caacaatatt taaatctggt actacatttt
     1921 ttaatgctaa aaatccaatc atagctccaa gtacagttcc tacagaacct acaagaaata
     1981 taatgagcaa tcttccactt tctttccata tctttttaat attacattga tataacaata
     2041 aagctatggc taaaggaaca acatatcccc atacttggtc atatacaaca gactctactg
     2101 gtataatatt tagatttgat aataacattg ctcctacaag agctattatt gctcccgaca
     2161 cttttgatgc ccaattgtac ttttgttcca agtaaatact aaatgctgcc catccagtta
     2221 caactgccca taatacccaa atattatcag gacttacaaa actcttcatc ttaattacct
     2281 ccatttcatt aaattaaaat atcattatca atattcaata tattgaatat taatactatt
     2341 ataatgcatc ttttcagaaa attcaatata acataaaaaa tacatgcatt ataaaacttt
     2401 tttttatttt attaatcttt ataaattata tgttataatc atgtctgagg gggtttgtat
     2461 ctaatgaata tagatgtcgg aagcaaaatt agatctctta gaaaatctaa aaatataagt
     2521 atctctactt tagcaaaaaa ttcagattta agtacaggct taataagtca aattgagcgt
     2581 aatatggttg ttccatctat tgtagcaatg tggaaaatat caaaagctct tgatataaat
     2641 ataggttatt tttttgaaga gattggaaga gaggactctg atatagtagt aaagaaaaat
     2701 aatcgaaaaa aaatagtaac taatgattct acaaaatttt atgaacttct tgttcctaat
     2761 ttatctggga aaaaaataga atttatactt gttacgctag atggtcatac acaaaaaaac
     2821 agtgaatttg tgacacacga aggagaagaa tgtggctata taataaaagg taaaatgaag
     2881 ataacacttg gaaataagga atatctattg gaggaaggag atagttttta ttttaacagt
     2941 actataccac atgtgtatga aaattatgat gatgaggtat gtatctcagt ctgggcaatg
     3001 actccacctt cattttaaca tattgagttc ttacttaaat ataaattgat aaaaatctga
     3061 aataaattag tatgataaac tttactataa tttatttcag atttttctag tttattattt
     3121 atatatcagt cattatatat taagtaggca gttctcgtct aaaatgttag ataatatctc
     3181 ttcagttcca ctcattcctt tgaaagaact tgatataaat gaattttcta tttcattttt
     3241 atttatatca ccatgcttca atacatagga tttatttgag atattaagca taccatagtc
     3301 cataatagaa tctccaactg cataaaaata ttctattcct aattctttag tcaagtattt
     3361 tatagcattt tcttttgaaa tttcttttgg tataaaatat atttttctgc cactcacata
     3421 aaacttccaa ttttttgatt ccaatatatt tgtatattct ttgattgaat ccaaattaaa
     3481 tcttgagaaa tcaacaacta tataaaaaaa tgtatcttct gcaactttaa aattagttat
     3541 tccatctaca ttcaaatagt ctttaaattt atgaatcata ctttcaatgg gctcataatc
     3601 aggttttagt ttatctattt tttcactcca tgatttcaat atttcattat ctttaagtat
     3661 aactcctcca tttgaagtta tactccaagg aaaataaatc ccatatttat taaattctat
     3721 acgcttaaat tgctccactg ttctagtagt tgtaggtata aacataccat aatattgtat
     3781 ttgctttatt aaatttatag tatctaaaga tatataagat atttcttttt ccctataaat
     3841 ttctatatta gcatatttac tatcagcatt taaaaattta ttggagtaaa tgatactcct
     3901 gtctaaatct gaaaaaacaa tcattcaata ttctcctgtc taatatattt cctaaatatc
     3961 tttcatattt tttattattc cacaacattt ataggctttt aaatccattt gttcaacttc
     4021 aacatttttc tccttagcta atatgaatat atggtctaaa gatttatcat ttaagtcttt
     4081 aacaagaatc ttatacggaa ctcttctaag taaaaccctt gtcgtttccc ctacacctgg
     4141 tttgataaag ttaatatctg ttatgttata cttttctttt atatttaaaa catcaaagta
     4201 tccatctctt gttataatgt cactttccca gttttcaact tcatcagata tattcttaaa
     4261 ttgattttta aaacattctt ctatagtttc gatatagttc atagattcat ctttatcttt
     4321 taaatgtcta taaaacttag ctccatgaaa gtcattttta ccaattaaat cctctcttag
     4381 tacagttcta cttactaaac cagaaacggt tgaatttaaa catgcacttg gtattaagaa
     4441 atcttctcta attccataaa gtcctgaata tccagcaggg tcagctaata cagcaagtgt
     4501 tgagtcaaaa tgttttttaa atttaacatt aagttcatca catgcttttt caagttcttt
     4561 tgatatagtt cccttaccag tccatccatc taaaaattga atttttgagc tcggatgatt
     4621 ctttactata tattcaattg cattcatatc tattccctta tctcttatta tagagattgt
     4681 atagtgaggt aaacttacat tatacttgct tcttatatat ctttttgcca aaataccaat
     4741 aggtgttcct gctctagcaa gtgaaactag tactacatca tttccatttt ttttaattat
     4801 tttttcacac ataactccta tagaaaatgc aagcttatct ttattttctc ttagtttctc
     4861 atgatataaa ttcagataat cttcactaac ttcatactct attggtatca tttctgaata
     4921 atgtctacca ttttgtattt ttaattctct ttctttattt ccttcttctt caattaaatt
     4981 tgatatatct tttaataaaa aaataacatc atctttagaa tacgtgctaa aatctctcat
     5041 atgactcaac tcctatctta aaatcgtgat atattttatc ctgttaaaac actttagcta
     5101 caacatacta tagttaattc ttttatactt gtttttttaa ttatatcaat aaattctaga
     5161 taagaatttt cacttttatt cagctctaca aataaataac acttagaaaa attatgttta
     5221 tctatattgt aaatataatt ttgaatatct atattataaa aactctttag cataaattta
     5281 cttttaatag gataatgctt cttatcaata ttaataattg gacttcttgt tgttgaatga
     5341 tagtatatat catattgatt ttctttttct tttgcaaaca gcataggtat atacataaat
     5401 tcttcagtac ctaaaaatag aatcttttct ttttcatttt ttaaagattc tttactggcc
     5461 atagttattt ttttactttc tctcttaacg atttctttta atctaaattg ttctcttcta
     5521 ttaattccaa atcttcctgt atattttaca tatttcttat ttccaatata atcttctaaa
     5581 tcaacataaa tatgatttac tactaaattt actttatcac ttgaaatagt ctctaattta
     5641 gtaatactat aattatcgtt gtaatttgaa ataatatctt catctatata aaatttaaag
     5701 tttcctttaa ataagtatac aaaatctatc ttacaattta gctctctttc taaacgagta
     5761 actttactta aattttcatc acttacccaa ttgagtattg agcatatagt gtatttcttt
     5821 ttaggataaa tgctttgaat tttcctaatt atattcatgc atgtattggc tgtggtaatc
     5881 tcatcatcaa ccaatattat ctcatctcca aatttaaagt tgttaagcat accaaagtat
     5941 aaattctggt ctgttgcatg ggaatgttcc tctaaaaatt ccagattttc taactcgtca
     6001 aactgctctc tagtagtatg aataaattca taatctccac ataagtaatc aaaaaaacta
     6061 tgcccaacag ctgtaccagt ttcagcaaaa gctataacag taccatttga ttcttcctta
     6121 tttttttcat tataaattgt agccattaat tttcccaaat tatccatctc acttggctta
     6181 catgctaaat gtttaccgag tttctttgag ataaacaaaa aacgtctttt gctattattt
     6241 cttactccta cgtctaaaaa atcatttagt tctaaattca atggattatc aataatgtca
     6301 atctcacaat taaaatttaa gcaattcttc atagtctact cctttattta atacaccata
     6361 tattttagat agaatcaatg tttttttagc ccataaaaaa tgtggatttg tttcattcat
     6421 tctctctcct aatacaccct tgctcacacc atacttactg tctgtactat ccagtatatc
     6481 caaagcatca gtaaaatctt catatgatac tgtagatagc gcttgtacaa tttgaatttg
     6541 cattggatga attacggtct ttccaattaa tccatgatat ttatccaata atatttcttc
     6601 tatatactta tttcttatat ccttatttgt catatcaaaa aattctgata ctccaccact
     6661 tataggtata tctaactctg gtctttgaag atatgtgatt atatctaata taaatgaaga
     6721 acaaatcaaa ttattatata tagagaagtt tttatctctc cttataccat acatccctaa
     6781 tatatcagtt atgcctactc taatgcttaa tattcttgac ttatgtctta aaatagcatt
     6841 gtacaaatta gtaaaactaa gttctttttt ttcattatat ataaattctc ttgtctctat
     6901 tattggaata acatacatat cttgaagatt catagaatat aaagcttcta tacaattttc
     6961 aatcaactcc gaatttgctt taggaattaa tattcctgtt atagaatgac tatttttaat
     7021 tataatctct ttaattctca acagttgctc cacatctttt attcttacaa aaattagtgg
     7081 tattccatct ttattagtta tactttcatt gctaatattt tttaaaatta atctaagatt
     7141 ttctatagcc tctaactctc catttactcc aacagcatct tctaaacaaa tagctaatgg
     7201 tctaactccc ttaacatccc ctataatact cttataaatc atattatatt gagtagctgg
     7261 aacgtataaa aaagcgccca gtgcatattt caacacatct ttttcagtct tagcactaaa
     7321 gtcttctggc tccttcaaaa aaattctctc caaatcatct ttgcagatat aatcgaaata
     7381 tttcaatgtc tagcctcctc atatacatag aaagggccaa ctttatacaa attagccctt
     7441 aatataatac tacttattat aaattatagt cgtatttaat tcttccaatc tctttgtatt
     7501 atccttaatt aaagctctat tgttttcttc tattctcata gtttcatcta taccagactt
     7561 gatagtattg tacatttttt ccaatgtttc tatttgaaca gaacttgttc cagccattct
     7621 agctatttgt gcactttgag tacttacatt ttgtgcattt ctaagcaata attcattagt
     7681 tttgtcatca agtgctttta gagattttgc ttgtaattct tgctttttaa gtaatatagc
     7741 ttgagataaa cattgtttaa atactggtaa agttacgaca aatgcactat ttatttttct
     7801 aattaaacca taattattat gttgcatacc tctaagcata ggtattgttt ggatagctat
     7861 attttctgct attcttaaat catagactct ttgatttaac atatcataaa cttttaatag
     7921 ttcttgataa ttgacaacat ccatttggtc tccactagtc tctgattttt gtttaaagaa
     7981 aggtagtatt tcattatcca tttcttctat tgccatttct ccagcaacaa tatatttttg
     8041 aagttcatta taaaactcaa agttttcatt taacatagca cctatttgct tattagaaag
     8101 tgctatatct ctttcgtatt gctcaagctc aatttgaatt ttctcaactt ctcctcccaa
     8161 tgtttcatac ttttgaaaca tcatttcaat ggcattatta gcttttttga aaagtttttg
     8221 aacgaaattt ggttccttag tttcttgaaa atcgtctaaa tcaaacttat ccattatttt
     8281 agttaaatga accagcattt tagaattttc ttcatttcta tttaatttta ttgtatttaa
     8341 aaggctatca gaaactcttg ctactccttc agatgctttc ctaccaaact gtaaaatcgt
     8401 atctggattt tctacttcta taagggatgt caatgcctca acttccttag actttctcag
     8461 ttcatttttt ttattatttg tatattccat tatatcgaag ttttcttctt gaactgctgt
     8521 aggcaaattc tcagtaacct ctacagagtt cttatctttt gaaaaactat ttatatttat
     8581 ccccatctaa aatcgtctcc tctttccaaa aattttttta agtaaaggta tatcattttc
     8641 aagcgaattt aatttaaggt catataccat tttatccaat ctgtgtatat caatcaattg
     8701 gttatttata acattctcta tattgttttc tcctcctgga cataatatga taatatcaaa
     8761 tccaatctta ctaagtaaaa gcataagaaa aactatttct ttatcaaata caatactctt
     8821 gtacatgtat ataatcaatt ttgggtttat aagtgagtaa tcgaaattgt tcatcatatg
     8881 aacaaatttc ttatccatgt gaagtactgt aaatagacct tttatcttgt cttctccact
     8941 taaaccattc aaaaacatac ttccttctaa caactcttcc actttctcta aaatcataat
     9001 ttgttgatta agtgctagtg ttgatatgga ataatcttta ttatttaaaa cagagttttt
     9061 atctattaca ccttttgaat ccaataaaaa ggataaacta aaagcttctc tcgtaaaagc
     9121 ttttgaaaat ctatctacat ctccattaaa ttcaataaat gttgaacttt ccgaatctct
     9181 taataaatct aaaaacttaa tatattcatt cttatcatta tatattccat taatcttaga
     9241 aagaaaattt ggagcttcta caatagcatc attaaactta aatcctggtc ttaatttaag
     9301 tggttgattc cagtatattg atatttcatc aacagttgaa cttagaagta gattttttat
     9361 ttttctatct tggagttgcc aaggttttat aaatcctgca tcatttaaca attcctcact
     9421 tatacgcttt gatgcttctg ccccaacagt aaaagctttt tttacagaac ttttatctat
     9481 tttttcccct aaaataaccc tttcttcaaa agaaattttt tcttctatta tgttggcttc
     9541 ttcaaactct atattgtatc tttctgattt taaaatatct atattactct ttgaatttgg
     9601 attaagatat aaaacatcaa aacctgccaa gtataatatt aagagtaaat aaacttcatg
     9661 tttttttata tctccataaa atattacttt tggagcttca tttttattta tatctaaagc
     9721 accgatataa atttttatcc atgccattat tttcactata aaattttgtt taactctatc
     9781 attagtaaat tctttttcct cttcttttaa cactctatcc attgcttgct ttattttata
     9841 atcaatctct tcaaatccca aaatatttat atctacatct tccatgctag ttctatattt
     9901 ttgaagtcca cttttaaaat aatttataat atcaaaatca ctaggatttg ctataacatc
     9961 agtaaataaa gcaatatctt ttttgctatc aatattttgt tttgtcttaa atatctcgag
    10021 attgtacaaa acttcatctt ctaaaccaat atacgaaata aaataactta gatatccctt
    10081 tgaaggagtg taatttcctc tatctgattg gctggagaat attttttcaa atacctttga
    10141 gtcacccata actatcctcc caatttaaat gtttattcag aactgaccca aaattcatat
    10201 tttgagtcag tctgttttct tctaaaaaat ttgttttaag cagtcttttt tggttttacc
    10261 agtcttatta tacattttac aacttcatta accacaatta cacttgctgc tacacataaa
    10321 acttttaccc atgtcataaa atccattgct acagagttaa agaaatcttg gaatagctga
    10381 gtaaatatta cttgtgcaat tcctgtcaac actattattt gtagtgctaa tttgttatgt
    10441 gtaaaattag ggaagatact atctgcatta aactctctac agttaaatgc attaaacagt
    10501 gcgctaaaag caaataacgc aaacattact gttcccttat gagcttcatc aactcctaag
    10561 aaattaaatg tagattgtaa cattagtatt gctgttatat aaaatgcatt taatactatt
    10621 gtatacacca ttgacttaga aattatattg gcttttctat caacaggttt tctatttaac
    10681 acatggtctc taactggctc taaacctaat gctagtgctg gtggtccatc cataattatg
    10741 tttacccata ataattgtat tgtagtaaat ggcatctctt gtcccataac ttgagatatt
    10801 atagccacta aaaatgcaat tacatttaca gttaattgga attgaacgaa tctttggaag
    10861 ttatcataaa ttcctctacc ccatttaata ccttttacaa tggtactaaa actatcatca
    10921 gttaaaatta tgtctgctgc gtttttagat acctctgtac cagatatacc cattgctata
    10981 ccaacatctg cttttgaaag tgctggagca tcatttatac catcacctgt tactgcaaca
    11041 acatccccgc tcttttgcag agctgaaact attctcattt tactatctgg tttacttctt
    11101 gccacgattg aaatactttg tatctcttgt tttaattctt catcatctaa agtatctata
    11161 tatgaagctt caaccactct catattattt tttaataggc ctaactcttc acctatagct
    11221 cttgccgtat ttatattatc tcctgtaagc atttttacag aaacaccagc tttatttgct
    11281 gtatttacag actctataac atcttttcta agtgggtctc ttataccaac aaaaccacta
    11341 aatactaaat tattttccat tgcacttaca ttcatagcat cactttcagc agttactgct
    11401 gcttcttctt cagcttgtga gatttcttta tatgcaaacc ctaaagttct cattgatttt
    11461 atttgcaatt tctttatttc ttctaaaatc tcatgtttta tattttcagt taatggaact
    11521 atgtcttttc ctctttgaat atgtgaacat aattcaagta agacttctgg tgctcctttt
    11581 gataatagta catgatgatt atcttgacct ataacagaag tcattctctt tgaatctgaa
    11641 gtaaaaggat tttgagctac tatattagaa ttttttctaa cttgtctata atctacgttt
    11701 ttgtaatata aaagcaatgc acattcagta gcacttccta aatacttaat atcaccatca
    11761 ttgtgctcta catcagctgt agagtttatc atacagtttt cttcaaaata actatttatt
    11821 tcctcttctg aactttcaat atatctgcca tcaacatatg caacttctac agtcattcta
    11881 ttttcagtta atgtaccagt tttatctgaa catattacag aaacagaacc tatcgtttca
    11941 caagcttctt ttttggtaac caatgcattt attttagcca ttttttgcat tgttattgca
    12001 agtgtcatat ttatcatagt tggaagacct tctggcactg ctgcaactat caacgcaaca
    12061 catactgtaa atgctgtttt aactgggtct attgattcta aaaacggtaa tattccagac
    12121 atatctacat ttaatacatt cttacttatc atttggaaaa ccataaatat aaatagtaat
    12181 cctgctactg cactagaaac tttggctatc aaaccaccta aatttccaag ttttatctga
    12241 agtggagttt gctcatctgt ttctgataag ttttgagcaa ttttacccat ttcactatta
    12301 tctccaattg aagttacaac tagagctcct ctaccataag ctacaagtgt accaccaaac
    12361 accatattta tctgttttgc aggtattggc tcctgtacta ctactgctcc tttagattct
    12421 atctcttcca tagaaactac tacatcacac tttttagata cgtcatcaga ttctcctgtt
    12481 aacatatctt ctctaatttt aaggtttata ctttctataa gtctaccatc agcaggtacc
    12541 atgtcaccag tttcaatata tactatatca cctggtacca attcactctt agaaatttga
    12601 tgtacttccc catctctcaa tacttttact tctatgtttt cagtcaactt agaaagagcc
    12661 tctgctgctt tcttagaacg accttctgta atcataccta ttgctatacc tattgctata
    12721 gcacctacaa tccctattgc atcatgagtt tccccaatta ctgcacttac tactgctgca
    12781 ataatcaaga taactatcat tggttcactt aaactttctt ttaaatcatc ccaaaaacta
    12841 ccctcttctt ttggagtaaa ttcattcaaa ccatattttt ctcttctagt cttgacttct
    12901 gattctgata aacctttttc aggattaaca gataagtctt ttgtaacttc ttctttatct
    12961 acattataat atttgatatc attattaaat tccatatgca cactcaatcc tgtctaaatt
    13021 ttgcattact tctttaagac ccgcatcttt tgttccttca ccaattgctt taaattgcca
    13081 acttccatct tttctatata tagctcctac tataagagta gttttttcag aataattatc
    13141 agatagattg tattttgcta tctctttctt attaccttgg tcttcaactc ttatataggc
    13201 attttctatc attccaaagt gttgttttct atctacacaa ttgtatatat ttactacaaa
    13261 caataattta tgaatatttg atggaacttt atccaaatta actaggattt gttcatcatc
    13321 acctgtaccc tcaccagtta gattgtctcc tgtatgcaaa actgatttac aggcactttt
    13381 tagatttcca aaataaatta aatcttttat tccacttaat ttaccttctt gattaagcat
    13441 aaatacagat gcatcacaat ctatatcagc ttgtcctcca ccaaataacg atttaaaaaa
    13501 acctcctcca gattgttgca cagggtccca tcctagacct acaagtatgt tactcagtcc
    13561 tgggttagat ttttttaaat ctattttatc accttttgat aaattaatcg acatactaaa
    13621 cctccacaaa attttatata atataaccta tacattattt gtataggtta tttatattta
    13681 atatattata ttgcttaaat taaacattta agccatagtt tctacaaagt gcttctaagc
    13741 caccttcaaa tcctgctcca actgcactaa acttccattc tccattatac ttatatatct
    13801 cacatactgt aattgcagtt tctatactaa attcttcacc taagtcatat cttaatattt
    13861 cttcatcatt ttcagaattt aatactctta tatatgagtt gcttacttgt ccaaaatttt
    13921 gtcttctttc agcagcctca tatattgtaa ctgttacagc tatcttttgt atatgctttg
    13981 gcattgtctc taaattcact aatatttcct catcatctcc atcaccttca ccagttctat
    14041 tatcacctaa gtgttcaact gctccagatg gatgtcttaa gttattgtag aatataaaat
    14101 cttcatcttt ttcaactctt tgagattctc caaccatgaa aacacttaca tctaaatcat
    14161 aatcatagcc actatcaaat gaatttgtat cccatcctag tccaagtttt atatttttaa
    14221 gacctggatt tcccttagtt aaatcgattt tttgtccttt ttttaatact atagccatgg
    14281 aaaatatccc cctttagttt attgttatag ttgaaattat aagtttattc caaagtttcc
    14341 acataatgca gcaagaccac cttcaaaacc agaacctagt gcattaaact tccattctcc
    14401 attgtgctta tatatttcag ctactactat agctgtttct atactaaaat cttctcctaa
    14461 ttcatacttt attaactctt catttgtgtc tttatctact attcttatat aagaattact
    14521 tacttgtcca aaattttgtc ttctttctgt cgcatcatga attgtaacag taaaagaaat
    14581 cttgcttatt tctccaggaa tcttagataa atcaacaact atttgttcat catctccatc
    14641 accttcgcca gttctgttat ctcctagatg ctcaactgct ccagatgtat gttttaaatt
    14701 gttgtaaaat acaaaatctc catcattagt aacacttcca cttgctcctg ttaaaaatgc
    14761 tgctgtatct aaatcaaagt caaatccacc atcatacttg ttagtatccc atcctaaacc
    14821 tactaaaata tttttaagac ctggatttcc tttagttaaa tcaacctttt ggccttttga
    14881 taatgttata cccataattt atcgctccct tgttaatttt agctaaacaa atcttgctaa
    14941 ttatttatct ttttctttaa tcatattata acatttttta ttcatttgtg aagaaaactt
    15001 gcttgatatt tttaaaatat aattaaaaaa atgtttatat ttcttacttt ttacatagat
    15061 tttacatact ttattaaa
//
LOCUS       DAMJVX010000084        14940 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.088, whole genome shotgun sequence.
ACCESSION   DAMJVX010000084 DAMJVX010000000
VERSION     DAMJVX010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14940
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.088"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(150..293)
                     /gene="scfA"
                     /locus_tag="PC407_002262"
     CDS             complement(150..293)
                     /gene="scfA"
                     /locus_tag="PC407_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HDF2497706.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(359..721)
                     /locus_tag="PC407_002263"
     CDS             complement(359..721)
                     /locus_tag="PC407_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HDF2497707.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(779..1072)
                     /gene="yajC"
                     /locus_tag="PC407_002264"
     CDS             complement(779..1072)
                     /gene="yajC"
                     /locus_tag="PC407_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDF2497708.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1206..2327)
                     /gene="tgt"
                     /locus_tag="PC407_002265"
     CDS             complement(1206..2327)
                     /gene="tgt"
                     /locus_tag="PC407_002265"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDF2497709.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITSIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPQDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2406..2891)
                     /locus_tag="PC407_002266"
     CDS             complement(2406..2891)
                     /locus_tag="PC407_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497710.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(2903..3928)
                     /gene="queA"
                     /locus_tag="PC407_002267"
     CDS             complement(2903..3928)
                     /gene="queA"
                     /locus_tag="PC407_002267"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDF2497711.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFLYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGNNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4246..5265)
                     /gene="ruvB"
                     /locus_tag="PC407_002268"
     CDS             complement(4246..5265)
                     /gene="ruvB"
                     /locus_tag="PC407_002268"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDF2497712.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5372..5974)
                     /gene="ruvA"
                     /locus_tag="PC407_002269"
     CDS             complement(5372..5974)
                     /gene="ruvA"
                     /locus_tag="PC407_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDF2497713.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASSAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(5967..6476)
                     /gene="ruvC"
                     /locus_tag="PC407_002270"
     CDS             complement(5967..6476)
                     /gene="ruvC"
                     /locus_tag="PC407_002270"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDF2497714.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(6788..7417)
                     /locus_tag="PC407_002271"
     CDS             complement(6788..7417)
                     /locus_tag="PC407_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497715.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKNNGVQTSK
                     VVSASANMDEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMNATIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            7620..7943
                     /locus_tag="PC407_002272"
     CDS             7620..7943
                     /locus_tag="PC407_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2497716.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            8211..9065
                     /locus_tag="PC407_002273"
     CDS             8211..9065
                     /locus_tag="PC407_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497717.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(9251..10687)
                     /locus_tag="PC407_002274"
     CDS             complement(9251..10687)
                     /locus_tag="PC407_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDF2497718.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNELLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPVETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTHGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKEMLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(10758..12140)
                     /locus_tag="PC407_002275"
     CDS             complement(10758..12140)
                     /locus_tag="PC407_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2497719.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAVLIGGWIYGKRFEKYPAYAIDENDREKN
                     LEKGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKRYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(12276..13181)
                     /gene="garR"
                     /locus_tag="PC407_002276"
     CDS             complement(12276..13181)
                     /gene="garR"
                     /locus_tag="PC407_002276"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDF2497720.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            13577..14242
                     /locus_tag="PC407_002277"
     CDS             13577..14242
                     /locus_tag="PC407_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2497721.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDVYKTTLIHMETPKYINLEDFC
                     NNK"
     gene            14350..14898
                     /locus_tag="PC407_002278"
     CDS             14350..14898
                     /locus_tag="PC407_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497722.1"
                     /translation="MIFLNENVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTIEILRKVLFERSEERAIAMRERALENGDELIADNIISTTDVPFLGWVPEFKE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
BASE COUNT         4911 a         2628 c         1726 g         5675 t
ORIGIN      
        1 gcataattaa acgataagaa atcaaattta attttacata agtagaatat atatttttat
       61 ataataattg aaaataaaag aggatacctc ataatgataa aagaatcact ttgagatacc
      121 ctcttacaaa caactaaatc aataattttc tatctttcac actcttgatt tgcaactgta
      181 caagaagttt tacaagctga ttgacaagaa gtttgacact ctccacatcc accttttgca
      241 gcactttgct ttaaagtagc ttgagataaa gtttttacat gtttattttc cattttcaac
      301 gcctccattt ctaattgttt cctattgatt atatcataaa actactttaa tataaactct
      361 atttaaagtt gacacccaat acgcctccaa tgcctcctgc tgctgatatt agcaataact
      421 tataaatcat ccctacttca aatacaaact tttcttgtgc taaaaacaca gtcaaaaaaa
      481 tacatactat gtacaaaagt cctactaaaa gtccatacat aagtcctttt tctttcatgt
      541 gttttgaagt gtaaaaccct cctatagctg cagaaatagt agttataaat gatgagacca
      601 tagttatatt atctccacca atatctgtaa atgtcaaaaa taagttatac actaataaaa
      661 cagccaaagt tattatatat gcatatccta gacctttaaa aatataatta gattttccca
      721 taaaaaaacc tcctctcaaa ttaatatata ttcgagaaaa ggcttttaaa ttactaattt
      781 agtcttcttc tatttcttct tttttagatt taacagttcc tatagcccat ttttcaaatg
      841 gaactttagt tctatttggt cctaattcta atattacaac atcatcttca actttagcta
      901 cattagctac tattccacct attgtaatga ccttgtctcc tacacttagg ctacttctca
      961 tttcttttaa ttgtttgtct tttttctttt gaggtctaat cattaggaaa taaaagattg
     1021 cgattactac tacccataga cctatactca taattatttg ttgctgtggc attaattttt
     1081 cactcctttg taaattatac tcttttgtaa atcataataa atgtttatat aacaatttac
     1141 tttctctatc tttgagtact attgaatatt atatattaaa atgattataa ttaatagtgc
     1201 aaaacctata tttcatatcc atattttgca aaaaattcat ttctaaaatc aagtaatcta
     1261 tcttccatta tcgcttgtct tatttgtttc attaaattta ataaaaagtg aagattatga
     1321 gtagttatta atcttgcacc cagtatttca tttgctttta taaggtgtct tatataagct
     1381 cttgaatagt ttttacaagc ataacaatca cattcagggt caagtggagt aaagtcttca
     1441 gcataagttg catttctaac tactacttta ccttggctag tcattgcagt tccatttcta
     1501 gcgatacgag taggtaggac acaatcaaac atgtctactc ctcttataac accttccact
     1561 aagtcatctg gactaccaac tcccattagg tatcttggtt tatcctgtgg cataagtgga
     1621 gttgtatgct ctagcacgtc atacataagt ggttttggtt ctccaacact aagcccacca
     1681 atagcatatc ctggtaaatc aatactagtt atttcttttg ctgattgttc tcttaagtct
     1741 ttatacatcc caccttgtat tataccaaat aaagcttgtt tgtcggtatt attatgggca
     1801 tctttacatc tttttaacca tcttgttgtt ctttctaaag aattttttac atattctcta
     1861 tctgatgggt atggtgcaca ctcatcaaac gccatcatta tgtctgaacc caatgcattt
     1921 tgtatttcca tagctttttc tggagttaga aaatgttttg aaccatctaa gtgtgaccta
     1981 aattctacac cttcttcttt tatctttctt aaaggcccta agctaaatac ttggaaccct
     2041 ccactatctg taagtattgg tttatcccag ttcataaact tatgaagacc accagctctt
     2101 tttataagtt catgacctgg tctcatatat aaatggtatg tgttactaag tattatttgt
     2161 gaaccaatct cttttaattc ttctggtgtc atagatttta cagttgcttg agttccaact
     2221 ggcatgaata ttggtgtttc aataatccca tgtggagtgt gtaatcttcc taaccttgca
     2281 ccactctgct tacaagtctt tatcaattca tatcttactg cgtacataaa gttctcctta
     2341 accatattta tttctcatta tttcttaaga tatatttgta ataagctatt catataattt
     2401 attgtctaaa tagatttatc ttcgtcttct ttgttctgtt gctccttaat tgcttcttca
     2461 tcatgaatac tcattatatt aagagcactc ttgtcttttg ttactaaaaa cttttttata
     2521 gcaaatagtc ctgctatagc tataactcct attaaaacat ctatcatacc ttcattttta
     2581 ggcagtagca acatttttct agctattgcg taaagtaatg cctcaataaa tgctcctggt
     2641 atatgaagag atagcattac tacaagttca acacctatta ctaaaagtaa tatttgtgct
     2701 aaaaaatcat taagctgact gtattgtact ggattttgaa aatcaactat atatgctccc
     2761 cacataagtc ttagaacatc tatagttcct aaaaatactg ctattagaac tactactgct
     2821 aaaacacgtt caaatataaa agcaatcttt aatatagggt tattcttttt ttgtttttgt
     2881 aaactatgca ttcattacct ccctacttta taatcatagc atctccaaaa ctgaagaatc
     2941 tatatctttc ttttactgcc tcattatatg catttaaaac attatccttt ccacaaatag
     3001 cacttactaa cattattagt gtagattctg gtaaatgaaa attagtaata agtttatcta
     3061 aaactttaaa tttataacct ggatatataa atatttctgt ccatccacta gtttctttca
     3121 tctttccatc ctcattacaa gctgattcta tagttctaca actagtagta ccaacacata
     3181 ttacattgtt tccattttcc ttcgctctat ttattttgtc agctgcttct tggctaacca
     3241 tataatattc tgaatgcatc ttgtgattta aaacatcttc tacttttact ggtctaaaag
     3301 tgcctaaacc aacgtgtaga gttacaaaag ctatatctac acctttttct tttattttat
     3361 ttaacaattc ttctgtaaaa tgtaatcctg ctgtaggtgc tgctgctgaa ccattatgct
     3421 ttgaataaac agtctggtat ctttcttttt catctagtct agcagtgata tatggaggta
     3481 gtggcatatt tccaagttca tccaatattt cttcaaaaat tccatcataa aggaatttta
     3541 taattcttga accttcatct gataaatcta caacttctcc aattagcttt ccatttccaa
     3601 aagaaaattt agtccctact tttgctcttt ttcctggttt tactaaagcc tgccaagtat
     3661 cttcctctgt tctttttaat aataaaaact ctattttacc tccagtttca agcttctctc
     3721 ctattaatct agctggtata actctagtat tatttaatac taaacaatct cctggattta
     3781 aatattctat tatatctcta aatactttat gttcaatatt acctgtctct ttatctaata
     3841 ccatcaatct tgagctagct ctatcttcta taggaacttg agctataagt tcctgtggta
     3901 agtcaaattt aaaatcactt gttttcaatg ttaaacttcc tctcttaaaa tttctccagc
     3961 tacaataata tcaaaaaaac aacttatttt ccacaatatt accataatct atcaagaaat
     4021 tgctattttt aacagacacc atatccacaa tatatagata tttttccacg atatacatgt
     4081 cttatccaca atatatagat acaatacaca caatttttat cgtgtttatc cacagtatct
     4141 acagatataa ttaccatttt gtggatatat ttgaattttt agccatttta gaaatatttt
     4201 aatttaatga ctcttttatt ttataaatac tcgtataatc tatttttatt tttcattagg
     4261 atatggaatt tttaagtgtt catatgctaa tggcatggct actcttcctc taggacctct
     4321 atttataaat cctaattgaa gcaaatatgg ctcatataca tcttctattg tatttctatc
     4381 ttctccaata gaagctgcca atgtatctaa acccactggt cctcctctaa atttttctat
     4441 tatagtcata agaagcttct catctacaaa atcgagaccc aaactatcga ctcctaaaag
     4501 ttccagagca tcttttgcca ccttatcagt tatttttcca tcagctctga cttgtgcaaa
     4561 atctctaact ctttttagta atctatttgc tatccttgga gtccctcttg aacgttttgc
     4621 aagttctaat gctccattag actgtatctc tgcatctaag atgcttgaag acctaagtac
     4681 tatttttgaa agttcatcaa ctgtataata atccaattta caaataacac caaatctatc
     4741 tctaagtgga tttgtaagca taccagctct tgtagtagct ccaataagtg taaatttagg
     4801 aaggtcaagt cttatgcttc tagcagatgg ccctttccct attattatat ctaggcaaaa
     4861 atcctccatt gctggatata aaacttcttc aacacttcta ttgattctat gaatctcatc
     4921 tataaataaa acatcatttt catttaaatt cgtaagtatg gctgccaaat ctccagctct
     4981 ttctattgcg ggtcctgacg taattcttaa atttaccccc atctcatttg ctattatact
     5041 tgctagtgta gtttttccaa gacctggtgg tccatataaa agcacatggt ctaactgctc
     5101 atttctcgat ttagctgctt ctataaatat actaagttgt tctttggatt tttcttgacc
     5161 caaataatct tctaaagttt ttggtcttaa actgttttct atatctatat cttccatctt
     5221 cattgtagaa gttattatcc tattttcatc ttcaaaacct tgcatttata cccctcctct
     5281 cgtacttttg aaaatctact ttatatattt tcaaatttac ataaactcct taaatattgt
     5341 tttaaaatcc aaatattata aaatccaaat actatttcaa ggatttactc attatatatg
     5401 taagagcttt ctttataata gcttctgtat tcataccatc ttttttacat ttttgaactg
     5461 cctcttttga ttccgacaaa gtatagccta aagctacaag tgcatctaca gactcatctt
     5521 gtgatatcaa ggtaggtttt tgtgataata acgttggttc aaactctatg ttatttttgt
     5581 ctaccttatc cttaagctct aagacgattc tttctgctgt ctttttacca acaccaggag
     5641 ctttggataa ttttccaatg tcttcactta aaatatatgc tcctaactgt gctgatgatg
     5701 caaatgatag tattgaaagc gccaccttag gtcctatttt agatacagat gttagtaatt
     5761 caaacatctt aagttcttcc ctacttacaa aaccacaaag actcatatca tcttctctta
     5821 caattaattt agtatatatt tttgtggctt ctcccacttg taaattcatt attgtattgg
     5881 atgatacatt tattttatat cctatcccat tattttcaac aactatactg tctatataaa
     5941 tttcttctac tgtacctttt atataactat acatacttac cacctatatt ctttagagtt
     6001 ttttcaagtt tgtttgaatg agcatggcaa atagccacag ctaaagcatc tgcaacatca
     6061 tctggttttg gaactttttt tagacttaaa aatgaagtta ccatctgttg aacttgtgcc
     6121 ttatctgctc tcccatatcc aactacacct tgttttactt gaagtggagt gtattcatat
     6181 acaggcttcc cattatgagc acatgcaagc atagtaacac ctctagcttg tgcaactgtt
     6241 atagctgttt ttacattctt gttaaagaat aattcttcca ttccaacttc atctatattg
     6301 taattcttta ctattgtatc aattccttta tacacaagtt ccaatcttct cgatatattc
     6361 atatgggcag gtgttgtaac tgctccataa tcgattactt taaacttgct atttttgtat
     6421 tcaattatac catatccaac tatggctata cctgggtcaa tccccaatat tatcattttt
     6481 taaaattccc cctaaaaata aaagaaatac caaaatgtat ttctcataaa aacttatgtt
     6541 ctaaatcatt atatcataaa taatattaac aaatcataaa atgctctatg taaaatataa
     6601 aatcaaataa aaaagttata tcaaagcaag attttacctc aatataactt ttatatttat
     6661 tacaaataaa ttattgtatt tttataggaa ttaaatgtga ctacaatttt aatactaatc
     6721 tatttaatat taaattattt attattaact tacttagtat ttaacttgaa ataatattaa
     6781 tttactatta acttgcaaag tcttctaatc ttgaataggc ctcttcttca gtatccataa
     6841 ctacaccttt tttgatttca tcttgaactg ttttaggaag tgaatctact cttatttttg
     6901 ttttttctac taatgtttta cttccactat catcatattt atatagtgct atatttccat
     6961 tatcattttc aatagtatat ttacctttta tagtagcgtt cattgcttgt acatctttat
     7021 tatcactata tttagattct accaatatga tttcattttt atccatactc tttatttttt
     7081 tagttggata ttctttttta aaatatgata ctatttctga ttttgtctta tctaataatg
     7141 actctggaac tatcccagcc attgtaagtg ttctcttata gttagtttca ttttcgcctt
     7201 cactttgaag ccaaacttca caatttttat ttaatccaaa agcttctttt gacttttcgt
     7261 ccatattcgc actagcactt acaactttac tagtttgaac tccattattt tttaatcttg
     7321 tccctatata aactccgcta cttaatacaa atactccaac cagtattaag gcagatattt
     7381 tccaaggaaa taattctttt ttcttctttt caaacattga ttacgcctcc tttttctatt
     7441 attttctcca agctgtaagt ttttatacaa tttaggtaaa ttttagaaga aaatatattt
     7501 atatttcaaa ttactctaag aatagatatt ttctaaaagg tagttttctc ttaatggtta
     7561 tattcttgta attatatgga cataatttat aataaataat cgtttaggag gtaataactt
     7621 tggaaagaat aaactgtaat gtaattaact gttcacataa caacagccat atatgttatg
     7681 cagataaaat ttttataaat ggtgaaaaag ctcacaatga cagacatact tgttgtagtt
     7741 catttttaga tgaatctatt tatagtaatt tatctagcaa cgttaataat agaaattgtg
     7801 aggaattagt ttgtactgta aaaaattgta aatacaacaa agatggtagc ttatgtacac
     7861 ttgataatat attggttgct cctgaaactg atagagtaaa cctttatatt gaaacatgtt
     7921 gttctagttt cgaatgtaaa tagtcatagt ataaatataa gggtgtcaaa tgtaattaaa
     7981 ctaacatagt gtagagtatc tacaactatt agtttgatta tacttgacac ccttatttat
     8041 tttaaataaa atttcattat tataaccatg tttataatgt tctaactgta attaaatttg
     8101 ataaatttct ataaattggt ataatcaata tgtaaattga atttagcaaa ttaatcataa
     8161 aaataaaata tcgagtatat agtgttatac taactagaaa tgaggtgctt atgagtacat
     8221 tttatattca atttctttat tttgtaattt atagctttat aggatggtgt tgtgaaacta
     8281 catattgttc agttttacaa aaacaatttg taaatagagg ttttttaaat ggtccatttt
     8341 gccctatata tggatttgga gcattagcaa tagttgctac cttgactcct tttgtacaca
     8401 atattccttt gttatttttg tttagtataa ttataactag cataatggag tattgtacga
     8461 gctttatttt agaaaaaata tttaatatga cttggtggga ttattctaaa cataaattta
     8521 atattcatgg aagagtttgt ctggaaaact cacttatgtt tggtgtatta tcattgattg
     8581 taatgcttat agtacatcct attgtggttg actttattaa ctctatctct aaaaatgtgc
     8641 tatttatatt tgctatatct atagaaattt attttgtttt agatttagtt atcacagtcc
     8701 atacaatttt acaattaaat ggtaagttaa aacaaatcaa tcttataatt aaggaattaa
     8761 aagataagaa agaatactac aaacttatta cacaagaaac tatagaaaac aaacttgaat
     8821 ctttagttga aaatagactg gatactttgg aagatagatt tgaaatactt aaggaaaaag
     8881 aaaactatcg ctatgctaaa aatcgtattg atgagctaaa aaataaactt aatactttgc
     8941 tgtctaacca caaattactt catagacgta taatcaaagc attccccaac ataagctcta
     9001 ataagcatac agatatttta aataaaatca aagaaaatat taaatcaatt aaaaaagata
     9061 aataataaaa taaacacata agttaaataa taaaaatact aatacataag attaaggttt
     9121 agaatatttt ttgtaaaata ctctaaaccc taattaatat actattattt ttaatcttaa
     9181 tttataattt tagtttattt tacttttaac acagtaaatt taagcgttta aacttcaata
     9241 cactaatata ctaccccatc aatatctcaa ctgattctct aagtgtgcta atctctccca
     9301 catttccagg gaaaattata tatggcatat taggaaattt gctttctttg cctgtcatcc
     9361 atactggtat accaggtttt atctgtccca ttactgtagc tttttttact cttagtgctt
     9421 tagtaccaac atcactagaa gtaattccac ccttagctat aataaaattt ggtcttacat
     9481 taagcatacc aattatactg gtaactgcat ctgatatttc tacagaaatc ataagctgtt
     9541 tatctttatc attcgtatcc aagtcaaaac gctctcgtct agtataaaca gcaacagttt
     9601 taccactgct tattttttcc tctacaactt ttataactct ttttacctca ttttttagac
     9661 cattttcctg aagaactaaa tgttgattaa attctataaa ttctattgga tatttacaat
     9721 ttttaagttc ttctaactgt tgggtagtct tattcacatg tgaaccaaca agtataattc
     9781 ctccattggt attacctttt gatactaact catccttagt aagcatttcc ttatcactca
     9841 ctccgccaag tacttttgtt atggcagcag cgcttctaaa cataaattcc ttaccactat
     9901 ttactgctcg tataaacgca attgtaaata cctttacatc tacataatcg gttgcattta
     9961 caataatttt attgaatcct tcagcttttt taagtttttc tgttatagct tcaatattta
    10021 gctctctaag ttctttagaa gaaatatata tcatatcact agatttgtac tttccatgtg
    10081 tcttttcttc acaccactca cctatatcag atgatttata tccaaaagat ttatcctttg
    10141 caaattcagt cattcctgct ggtacaagta tttctccttc tttaacataa tgtacattat
    10201 ctatagtaaa cctaccacct tctttaaaaa atggcattat tatttcaccc tgaaataact
    10261 tccccgataa tctctctact tcgcttttta ataaattagt ttcaacagga taatgtcctc
    10321 tcatagtaga atcactacga cttatgatta taaaatcctt attaactttc tttgatacat
    10381 ccacaatatt ctttgaaatt tctttatgtg cttttgtagt ttgagcaaca gtaaatcccc
    10441 ttgaattagt aagtataaaa aacatactat ttttctcatt aaaaccttgt tccatactat
    10501 ccttatccca atcagtataa acagaaatat catgaactgt ttgtacacct gttgggtcat
    10561 catctaatac aacaattttt ctatctaaat gcttaagctc atctgcaaga agttcattta
    10621 atctgtcctc atctacttgt ttaaaagtat taagtatatc tatgctaatt ggttttactt
    10681 ctctcattta aaacgcccct ttatgtaatt tgtatagtct aaatgtaatt tatataatat
    10741 aaacaatagt ttgttattta tagacctgga agtggtataa ttccatttat aacactaagc
    10801 aataaaacca ttataaatgc tacaactcca gcaatggttc ctccaattgt ccaagagttt
    10861 aaagtatctt taactgataa tccactaaat cttgaaagta cccagaatcc agaatcattt
    10921 ggaagggaga aaccaactcc tccagcacat atagcaagcc ctactaaaac tggtgacatt
    10981 ccagttgcca aaattgtagg tcctaaaatt gatgatgttg ttactaaggc tacagtagca
    11041 gaaccttgag aaattctaag caaagcactt aataagaatc caagcactac aactgatata
    11101 ctaagaccag acatagtatc aactaagaag ttacctatac cacttgcatt tataacactg
    11161 ccaaacgcac cacctgaacc tgttatcaat aatattagcc ctgctgaatc agcagcttca
    11221 ataactacat ctcccatagg tttatttatg tatctcttta aaaatcctat tgccaccaat
    11281 attccaagaa gtattgctat gtttttatct cctataaatg caagtactcc actcattgta
    11341 gaaccttttt ctaagaacat actaaataca tttgatataa gtataagaag tattgggaac
    11401 aataaagaaa acatagaaat tccaaaacct ggtactttag aagaatcatc atcttcaatt
    11461 tttatgctat cttttccctt ttctaaattt ttttctctat cattctcatc aatagcataa
    11521 gctggatatt tttcaaaacg ttttccataa atccatccac ctataagaac tgctggaaaa
    11581 gcaacaatta tagaataaag tataaaacta cctacatttg catttacatt tgctgcaact
    11641 gctaaaggtc ctggtgtagg tattacaagt gcatgaccta ctatagtccc aactcctaag
    11701 gcacatacaa aaaccattag agatttctta gttactctcg aaacatactt aattataggc
    11761 atcattataa caaaagctgc atccataaaa actggaattg aaattaaaaa acctgctgta
    11821 gttactgcta aagcactgtt tttttctcca gtcttagcta acaacccctt tgcaatagat
    11881 tctgttgctc ttgattctga aagaatattt ccaaatatta ttccaagtcc aattacaata
    11941 cctatactac ctaatgtatt tccaaatcca gtcgatattg tggttgatat ttcatttata
    12001 ggcattctaa ccataaagcc tgtaattata gatgttatca gtaatgccaa aaacggattt
    12061 actttaaatt taataatcga tacaagtaaa attcctatag atataacaaa tgctatcaaa
    12121 agtccatatc ctgtaatcat aatctttacc cctttatcat tctactttat tatttatcaa
    12181 ttttattttt cttttactat gataacttaa cctcgctgta tttatataat ctaatcctaa
    12241 taatatgtat ttaattatat tgatatattt aaatattaat tattgtgttt ttttatttca
    12301 acacctgcca acttttcaaa atactgtgct attccactat ggtcatcttc caaatgtcca
    12361 tgtactttta atgtctgcat aatttctaat aattgacttg tcataggtag cggtacatct
    12421 aaattgtgtg ctgtttgcat tacattctta atatctttca aattaataga tatttttcca
    12481 cctggtttaa aatcacgatt cataatcatt ggtatcttcg catccaatat tgtactacca
    12541 gctaggcctc ctcttattgc cttatatact ttttctgggt ctgcacctgc ttttgctgca
    12601 agtacaaatg cctcagaaac tgctgctatc gtcaaattaa ctattacttg gtttgtaagt
    12661 tttgtaacac ttccactacc attatctcca attaaaatag atgagcttcc cattatatta
    12721 aaaaatggct ctactttctt ataaatttct tccttaccac cagccataaa agcaagtgta
    12781 ccatcaatcg cttttggctc ccctccactt actggtgagt ctataaattc taagcccata
    12841 tcttttaatt tatttgcaca caatattgat tctgtaggag taactgaact catatcgact
    12901 actatacttc cttcttttaa agtttttgcc aatccatcca tcccaaataa tatatcctga
    12961 acaatagttc catttggaag gattgtaaat acaatatcac attcttgacc catttctaaa
    13021 acacttgcgt attttgctcc acatgatata agttcatcca cagcagattt attaatgtca
    13081 tacaccaaaa gattacatcc atcttttaat aaattttttg ccattggctt tcccattatt
    13141 cctaatccaa taaatcccaa tttcatttta taaccctcca tatcttcaca tttttgaaaa
    13201 cgttttttat gttttagagt atactacata ccagtatacc ggtcaatata aaatataaaa
    13261 gtatttattt taacaataaa tagtaatttt taaagtcatt tattatattt agtcatccta
    13321 tataagtttt aatacatact attgttttta aatataatat gctactttta cagttttgta
    13381 tttttactaa tttcaattag ttttaattaa tctgccttta atgttaatca tacttcacac
    13441 ttcaataatt ttttatattt tttaatctta tctgcaattt aaattgcaaa agattgacct
    13501 tattttcact atgttgtatg cttaaaatta tataagtttt tgtattttta tattgtttag
    13561 gggggtatta ttaagtatgg ctacatttaa aagattacaa gaacaagcat atgactactt
    13621 aaaagaattg atactatctg gagaaatgat agaaaatgaa atatattctg aaacaaaact
    13681 tgctagtgaa ataggtatct ctagaacacc tataagagat gctcttcaaa gacttagtca
    13741 agatggattt atagatataa tcccaagcaa aggatttaga atccatcaaa ttacagcaaa
    13801 tgaaattgta gagatattcc aaatacgctc tgcaatagaa gggttttgta cttttttaat
    13861 tacaagtcaa tacaaagaag ctagggcagt agaaactata agtaaattaa aacatctttt
    13921 agataaacaa aaagatattt tactaggtga taaaaattta aactcatttg cagaatacga
    13981 tacattattt cacacaacta tagtttcata tgctcaaaat actgaatttg acaaaatgtt
    14041 taacaactat atgtacagaa taaaaaaatt agctctcgat tcattatcac atgaaggaag
    14101 gcttgaaaca acattaaaag aacacacaga tatttttaat aatattgcaa atggatgtat
    14161 tgaagatgtt tacaaaacta ctctcattca tatggaaact cctaaataca ttaatttaga
    14221 agatttttgt aacaataagt gattttagta ttatactttc atttattaaa aatatatcta
    14281 cctcaaatta atttcctaat ttataaaaaa tattctataa ttatattgta actaatacta
    14341 ttctaaggag tgattttttt gaacgaaaat gttgtaacta atgaaactat tgacaaagat
    14401 tatgctattg aagaagttcg tatggcatgc aaacattttg gtgacttata tttttatttt
    14461 tccaaagtgt tatttgaaga gtttggtgaa gataaaacta tcgaaatact aagaaaagtt
    14521 ttatttgaaa ggtctgaaga aagagctatt gctatgagag aaagagctct tgaaaatgga
    14581 gatgaattaa ttgcagacaa tattattagc acaactgatg ttccatttct aggatgggtt
    14641 cctgaattta aagagttaca ttgcccttat ggagcttcct ggcttttaag atttgaggaa
    14701 aacccttggt ttaaaaaatt cgcttctctt tactgtgatg ttacagatac tactgtagct
    14761 gaagtattta caggagatac gtcccataaa ataacaaaaa acattctatg gggagataaa
    14821 agttgtgaaa gaatatattt ccatgatgac aaggtatctg aaggtaaata tacttatgga
    14881 aataaaaact gtaaataaat aatttaaatt ttctatattc atatactata ttttgtactt
//
LOCUS       DAMJVX010000085        14935 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMJVX010000085 DAMJVX010000000
VERSION     DAMJVX010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14935
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.089"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(410..2476)
                     /locus_tag="PC407_002279"
     CDS             complement(410..2476)
                     /locus_tag="PC407_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2497723.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVYTANRLIDKNKNRYEKVIKCSQGDGGSVTYISPHDSEEEAL
                     YIAKEIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(2553..5429)
                     /locus_tag="PC407_002280"
     CDS             complement(2553..5429)
                     /locus_tag="PC407_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2497724.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKILNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILNDKNINDKPIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQEGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRIEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKDKKLKWIRMNIFEDELKVLG
                     VVVDVTKEFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLD
                     NLKYINDTYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRD
                     IINSVKKQMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTI
                     KGEVSEFDINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPK
                     IKTFSSPIDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLS
                     KVDFEEIEDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNG
                     DAVLLYIMPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVISEGVETKGEME
                     TLINLGVDYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(5482..6942)
                     /locus_tag="PC407_002281"
     CDS             complement(5482..6942)
                     /locus_tag="PC407_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDF2497725.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTIGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIEEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLNVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFETWFESFKAELENAIKK"
     gene            complement(7034..7408)
                     /locus_tag="PC407_002282"
     CDS             complement(7034..7408)
                     /locus_tag="PC407_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497726.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKDNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(7586..8326)
                     /locus_tag="PC407_002283"
     CDS             complement(7586..8326)
                     /locus_tag="PC407_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2497727.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDSLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAVVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(8347..9135)
                     /locus_tag="PC407_002284"
     CDS             complement(8347..9135)
                     /locus_tag="PC407_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HDF2497728.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHIGVLFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSVDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEISE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(9223..9313)
                     /locus_tag="PC407_002285"
     tRNA            complement(9223..9313)
                     /locus_tag="PC407_002285"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9277..9279),aa:Ser,seq:gct)
     gene            9572..10336
                     /locus_tag="PC407_002286"
     CDS             9572..10336
                     /locus_tag="PC407_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2497729.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(10617..11732)
                     /locus_tag="PC407_002287"
     CDS             complement(10617..11732)
                     /locus_tag="PC407_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2497730.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(11773..13299)
                     /gene="glpK"
                     /locus_tag="PC407_002288"
     CDS             complement(11773..13299)
                     /gene="glpK"
                     /locus_tag="PC407_002288"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2497731.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFESGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKEIKMLRVDGGITKNNFTMQFQSDILDIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTKQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            13592..14689
                     /locus_tag="PC407_002289"
     CDS             13592..14689
                     /locus_tag="PC407_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2497732.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILFTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
BASE COUNT         4850 a         2636 c         1596 g         5853 t
ORIGIN      
        1 aatatctttc acattgttgc tttttcttaa acaatactaa ataatatctt tcacattgtt
       61 gcttttctta aattacacta aataatatct ttcacactgt tgctttttct ttaataatac
      121 taaatagtat ctttcacact gttgctcttt ctttaataat actaaataat atctttcaca
      181 ttgttgcttt ttcttaaatt acactaaata atatctttca cattgttgct tttctttaat
      241 aatactaaat aatattttcc acattgttgc tttttctaaa caactataaa taatatatta
      301 aatccttatt tctttttcta ataagttaaa taatacattt tacacatatc tatttgtctt
      361 gcacataata aataatacat tatatttcta tctaaattta gattctaaat taaatagtat
      421 aaattatatt cttcaataaa caagttttca aatccaagat tcgctctcta tctttaaact
      481 ttattgtaaa cataattcca tcttttttaa ttatttctcc ttcatgaaat tttttatggt
      541 ataccttatc ccctacacca atactgtcaa tttctttttt cgttggagat tttatatcat
      601 taataaataa tgatttatca actttcttac catacttatt taaagtagag cttatacata
      661 acttttcttc agctcttgta atagcaacat acataagtct tctttcttct tctaattgtt
      721 cttctttttt ctcttcctta caaatatcat atgatttttc atgaggtatt gttccttcat
      781 ttacacctat aatataaaca tttctaaatt ctaatccttt tgcactatgc atagttgtaa
      841 atatgacacc ctcagtctgc ttattcttgt tattttccgt taactcagtc tttactctat
      901 caatatgctc taaaaactct gtaacagtat taaaattagt tgctgaactc tcaagttcat
      961 ttaatatctc tacaagtcca ttagttttta tctttctgtt gctacaataa tctaaaacgt
     1021 atctatcata atcaagacta gttctaatat aagatattgc atttttagga tttaaagtat
     1081 ttaaataact taaatctatt tccaaatcat ttattgtttt aacttgttta ggatgaagat
     1141 tacacttatt tattaatgct gttataaagt ctttttcatc cttaaccata tttatactgt
     1201 ctttggatat atatctaaaa ggcttgttta taattctcaa ccaatcttta tttgattttg
     1261 gattaatacc tattcttaaa taagctagta tatcttgact tgcccaatgg tcatatattg
     1321 taattactga gtcttttact acaaatggaa tcctcatatc cataaataca tctactaatg
     1381 ctcttgactg tatatttgtt ctataaatta ctgcaaaatc tgaatactct acataatctt
     1441 tttgtatttc gtcaattatt tccttggcaa tatataaggc ttcttcctct gaatcatgtg
     1501 gagaaatgta ggtaacactt cctccatccc cctgactaca cttaatgact ttttcatatc
     1561 tatttttatt tttgtcgata agtctatttg cagtatacac tatctcactt ttagacctat
     1621 aatttatatc taatattatt tttttagtat tattaaagta ttgttcaaat tcaagcaaaa
     1681 aatctggtct agcacctcta aatccatata tactttggtc ttcatctcca actgcaaata
     1741 tattgtttaa tgggctacaa attaacttta aaacttcaaa ctgtacttta tttatatctt
     1801 gaaattcatc tatcaatata tatttataca catttctaac catttctagt gcagacttat
     1861 tgttcaaaag taaataataa gttcttataa gcatatcatc aaaatctatt ttgttaactt
     1921 tagatttttg ttcttcgtat aagttgtata cttttaaaaa ctcatctttt gttaatactt
     1981 ctgaattaaa ttcattttta tccataagtt cattttttac ataggagatt tcatttataa
     2041 cttgtcctat attctcatca tcatcagcat tctctatatt taaacttttt aggattgcct
     2101 tgattgtcat tcgctttgac ttttcatcaa atatactgtc taaattgtat ctttcaaagt
     2161 atcttagaat tttaaaaaat acagagtgaa aagtaccata ggttacctta ttaagtctta
     2221 tatcatcact taaattcaaa gctctatttt tcatttctat tgaagatgcc ttggtaaaac
     2281 ttattgccaa gattcttgtt ggtgctatat ttttgtttac aaccatattg gctattctat
     2341 atgtaattac tctggtctta cctgaaccag gccctgcaag tatcatgcaa ggcccatcta
     2401 tatgttcaac tgcttctaat tgattttcat ttaactttct tatatctatg tttgttgtga
     2461 ttattagctc caccacctta atttataatt atattatttt ctttttaatt ataaactaaa
     2521 agttcataaa aataaattta atttttcctc tattaaacca catcattcct attttcatga
     2581 catatcttga gctctttttt aattttgtca cttatatctt caggtataaa tgatggttta
     2641 ctaatatagt aaccctgcat ataatctact ccaagattaa tcagtgtttc catttcacct
     2701 tttgtttcta caccttctga tatcacttta atatttcttt cgttagaata tgagataaga
     2761 ttttttaata ttttctgtct gttttcatct ttatcaatac ctcttacaat agacatatca
     2821 atctttacat agtctggcat tatatataat agtacagcat ctccgttgta acctgtccca
     2881 aagtcatcta aagcaatttc tatattccaa tggtctatat atgctttctt tttctcaata
     2941 aaatcttcat taatcttatc attctctgtt atttctaata ctactctatt taaatattca
     3001 ccatataaat cttcaatttc ctcaaaatct acttttgata aattattatt aggaatagaa
     3061 tttaagaaca gtttgcaatc gccaaatttt tctttgtatt ttttaaatga tttaagtgct
     3121 ttaaaccatg taatacgctc aatttgatat aatttagact gtgaacgagc taatctaatt
     3181 acatctattg gtgaactaaa agtttttatt ttaggtctca taagtgcctc atatgcaaat
     3241 actttacctt tacaatctac aataggctga aatgcatact caactaattg accatcaatt
     3301 aacttgttta attcttctct attactcaat aagaatgaat ccttattata tatattaata
     3361 tcaaattcac taacttcccc cttgattgta tttttaattt gatacatagc aaaatctgcg
     3421 tatctcatta attcatcata gttggttgaa tcatcagggt accaggaaat tcctgctgat
     3481 gcccttattt taaattctgt tccatcatga agttttaata aggtattatt catctgtttc
     3541 ttaacagaat taataatatc tcttatacta tctttacttt catatccata tataaataca
     3601 taaaactcat cacctgaaag tctagatact ataccattcc atgatataaa cttcttaagt
     3661 atgtctgcag cacacctaat atattcatca ccagcatcat gtccatatgt atcattaata
     3721 tattttagat tatctaaatc ccacataata aatgctgcta tcttaagttc atctttattc
     3781 ttaaattttt cttgtatctg tgaatgaaaa gctctcctat ttaaaagatt cgttaaaata
     3841 tcaaaatctc tttcatattc tatcttcctt ttctcaataa attcttttgt gacatcaaca
     3901 acaacaccaa gaacctttag ctcatcttca aagatattca ttcttatcca cttaagtttt
     3961 ttatcttttg tacaaaaaac attctctgcg tttttgtata actcaaaagc cccgattggc
     4021 atatctacca tttcaataat ctgtgataat tttgatgaag aatctgcaac atttgcactg
     4081 agtaactcta tagctgaaga taattcatct atttcagata tatttatctt atcaagattt
     4141 actggttttg atggattact gtttctaact ttgttggcta aaagaacaat tggttttgta
     4201 aataatcttg atgcaatgta aatacctatt agacctatta ataaagaaat tgctatagat
     4261 attataacta atttctctat cctttttgaa aatgacaaaa gacttttttc ttcaataata
     4321 cccaaaagag cccaacgttc attttcaaat ggagtattgg agttatataa atctaagtat
     4381 tgtacactag cataaatgtt gccattacca cattcattat ctttattttt aatcttatat
     4441 atatttttat acgattcctt ctgctctaaa ttagaatgta aaccatccca aaatttttct
     4501 tttaatgagt atccatttga aattacattt tcaaaggcca tatcattatt tctatctaca
     4561 cctaaaatat aattaccttt tttatttcca tttatctctg agtatggaag taattctctt
     4621 aaataattta cacttaaatc tattccaagt actccatata cagttccatc ttcataaata
     4681 aggggaactg tatatgtcat aagcctatca cctgcatttg tcaataaaaa cttacttccc
     4741 cagtatccca aattagaact tgatacgttt ggattatcaa ttgcagctct aaatggttta
     4801 taaaaaaaat catctccctc ttgctcattt tcacttttaa atgaaaattt aggttcccaa
     4861 taactgtcca tagatattcc aaaacttctt gctatatctg atgaacctct ctcaattaac
     4921 aaatcagaat tatcatttgg attaaactta gggtctaaat cccttatata caatccagtc
     4981 ctacttattt cacctttact atctattggc ttatcgttaa tatttttgtc atttagaatt
     5041 ataaaagctc ctgtaacaga gttttttctc aaaagatata ttaaatcatt tgatatgctt
     5101 tttataatat ctttgttaat ttcttcattg ttgcctacat cctcaattaa ggcatttttt
     5161 tcatttaata ttttttttac agaggaatta attgtagctt ccgtttcact tgtatttgac
     5221 catctttgta acatttcatt ttgtatataa tttttacgat taattactct ttcatttaag
     5281 atgtcaaacg aattatcatt taatttctta atagttcctc cccataaaat tgtggcacaa
     5341 aataaacttg tttgaattag cataacaata attaggggaa atatcatctt tttcattatt
     5401 gaattgtttt ttcttagatt gttttccata cttattccac ctataccttt gtgcttgtct
     5461 acacactaac tgccaattca tctatttttt tatagcattt tcaagctcag ccttaaaact
     5521 ttcaaaccaa gtttcaaaat tcttatcatt attaaattgt gctaccgcat ccttcttaga
     5581 agttccactt tttaccaatt catttatttt atttgaatct tctttagctt tatcaagcat
     5641 tgaatattca agtatttgtc tagctttagt tccaccatca aatgctttat tagtgtataa
     5701 attacaactg ttaatttgtt caatagatga ggctattgaa ccttcaactt tatcagataa
     5761 actcaaatta tctttcttca taacttcttt tataacattc aaatcattcg tttttttctt
     5821 tactggtaaa tatccagaag caatagagaa ttttatatta ggctctttat ctgtaaacca
     5881 ttttaaaaat tctactgatg catattcttc ttctttagta gattttgtta cagccattcc
     5941 tgcaccttgt tgtacagcat attttccttc tcctttaaaa cgtggtgctt gaaaaacaga
     6001 tgtttcaata ttataactct cattatcatt tagcattact ctatctggaa agtatgctgc
     6061 tcctgaacta gagcctacaa gagctaatat atcacctgtt tttgcatcat cagaacgaaa
     6121 cttaccataa gaagcaaaat atccatttat atatggtaca tagaaattat cccatagctt
     6181 tttcataact ttttcttcaa tatttagtgt aactttacca tctttaacag aaaaaatttc
     6241 tactcctagt tgtttacttc ctataataat ataatttgct aaagcatctc tcccaaaaaa
     6301 tgctttacca tcattaggct ctttcgttaa actatctgtc cattcataat atttttttga
     6361 tgtctctact aatccttcaa ttgtttttat atcatttata tttgaatttg tagcttttgc
     6421 aaatttatcc caatcagttt tatttaacat caaaatttca gttgattttg caattgggaa
     6481 aagtttaaac tcatccttag aatttattct gccttcttct acatattcgt ctatgtattc
     6541 atttaattct tctttactta tgtacttatc catatctact agtaacccta atttatccat
     6601 ttcatatgca gtatctgagt atgctgcaaa tatatttggc acatcttcag agccaacttt
     6661 tttatttgca gaatctaaaa ctttctctga taaatcaact acattacctt gactaaacgc
     6721 ttcaacaata attccttttt cagcacctat agtgtcatta aattccttaa caagcttatc
     6781 aaaagccatt ttttgctgtc cattataata atgccaaatt tgtattgatg tcggattttc
     6841 tgggtctaac ttataattat cttccttttt taaactacat cctgtaagta ataaaacaat
     6901 cattgatgtg ataattaatt ttgcaaactt acacctattc ataaaatcta ctccttcaaa
     6961 ttataaaatt tataatagca ttaaaactgt aacttcacta tttatattaa ttatagaacg
     7021 tttattgata tatttaaaca ggatttacaa catatttaaa agcaattaag aaacatacta
     7081 ttacaaatat agaaaataaa gcttttacat aatttttatt tctaaatgca ccaaatgaca
     7141 ccaatccttg ttgaatagct actgtaatta ctaaaagtat cattattata ttaagttgaa
     7201 ccaatttaaa taagtaaagt aatgaaaaca ccactagaag tacacataat acaatactag
     7261 atttattaat aatatcatca atcttatcta ccttcttaga ctgtttatta tctttattta
     7321 atttttcttt atttaaattc tcaacttttt tattatatct ttctcttgtt ttatctttat
     7381 atttcgtatt tttattttta ttccccattt atttcccatc ctctataaaa tttttttaat
     7441 tgagcttata tattatttaa atattatata tttaagatac catatattcc tacaaaaagg
     7501 taacttacta tgtatctatt tttaattttc gcataaaaaa gccatctcct ttagaattaa
     7561 ttctgtattg agacagctaa tttaactatt tatcctttga aacattttct acattatcat
     7621 atttactatc aactaaaact atatcaactc ttctattttt tgacctacct tcttcagatg
     7681 aattttctgc tattggtcta aactcaccat atcctacagc agataattta tctggtgcta
     7741 tacctgcatt gtcaataaga agttgaacaa cattggttgc tctcataact gataaatccc
     7801 aatttgactt aaactctgaa ttttttatag acatattgtc tgtatgacct tctactctca
     7861 tgtaactatt catttcattt aacatcttac ctatttgaat aatcttatct ctagaattat
     7921 cctttacaac tgcttttcct gtatcaaata gtattgtatc tttaaggctt attactaatc
     7981 ctctatcttg aactcttaat gatactgaat tagccatacc attttcattt aaatacttct
     8041 ggatttcttc accaactttt ttaaacttag cattttgtaa actatctaaa tcattacttc
     8101 ctggttcaac tactggttct attttgctat taccaccttc aataactcca gaagaaccac
     8161 caaaagcaga atttagagat tgagataatt gtttatattt ctccgcatca acattactca
     8221 tagaatacat tattacaaag aaaatcataa gtaatgttat gaggtctgaa taagttaaaa
     8281 gccatcgctc attgttttct tctttttcat cttcatcata caacataaac attccatcct
     8341 tttatattat tcagctctac caccaatttc ttcaagttca attagttctt ttgaatttaa
     8401 aaatattttt aatttttctg tcattaaatt agaattatca ccctcaagta tggataatat
     8461 tgcttctatc accaaaagtc tttcacttat ttcctgttga tttatagcct taagttttga
     8521 tgctattggt aaccatatta aattggcaga accaacacca tacaaagtag ccaaaaatgc
     8581 aacagaaatt tgaggtccta atgcatctgg attacttgaa agattaccta aaatatgtac
     8641 taatcccata actgtaccta ttatccccag tgtaggtgca tatccacctg ctgaatcaaa
     8701 aattgctatt ccttctttat gtcgttttga tgttatctgt atctgtgatt ccaatatatt
     8761 tcttatagta actgcatcca ccccatctac cataagttca agcccttttc taataaaagg
     8821 gtctaattca tcattctcac ttatttcttt gtcaacacta agaagcccct ctttcctagc
     8881 ctttgttgca atttctttga aatagattat attttctatg ttattaggct ttttattttt
     8941 aaaagctaat aacattatct ttatagactt tttaaacaca gggaatggag tcgaagctcc
     9001 aacagcgcct atagttccac caaacacaac aagtgctgat gaaaatacaa ataatactcc
     9061 aatatgtccc ccatcaataa caaaagcaac taccaaagat gctatcccca ctatgaaaaa
     9121 aattaaagtt gtcatctatt ccctcctttc tttaattata catatctaat catttatgac
     9181 attataaaaa gactatcttt aataaaaata gcctttaaca attggcggag aaggagggat
     9241 tcgaaccctc gcaccggtta cccagcctaa tcccttagca ggggatcctc ttgagccact
     9301 tgagtacttc tccatatctc tatactagta aattatataa taaaatcata ttttagtcaa
     9361 ttgttttata aaatttgagt tattttgaat tattaaacat ctatttttac ttttttgctt
     9421 aaatgtaagt tttaaatatg gtaaaattat cttgcataac acttataata ttatttgata
     9481 aatatacata cattaacaaa tatacctaaa ctctgactta attgtaagtt atgccaaaac
     9541 taaaataata ctagtccaag gaggatattt tatgttacca caggaaagat acgagaagat
     9601 tttaagtatt ctcaaatcag ataatataat taaaataaat gacatagttc aaatgtttga
     9661 tatatcaata gaaactgcac gaagagattt agcccatatg gaaagtatcg gtcttttaaa
     9721 aagagtttat ggtggtgcta taccagttat agcaagtagc gtagaactta actatgttaa
     9781 aagaaaaaaa ataaactcaa acgataaaaa acagattgct attaaatgta gtgagtttat
     9841 aaatacaaat gatactattt ttatggatac tggaactact gtacttgaag tttcaaaaaa
     9901 tttgaaagat aaaaagaatc taactgtaat aaccaactca attttagttg caaatgaact
     9961 tatgaatact aatataaaag tatatgtgct tggtggctat cttagagaaa ctgaaggttc
    10021 tttttctggt ccacttacac tctcagctat tgaacaattt aatgttgata aagctatttt
    10081 aggtgctgga ggtatcactc ttgaaaatgg aatctctgac tatcacttag aagaagcact
    10141 ggttagaaaa aaaatgattg aaaggtctag aaaatctata gtagttgtcg aacctcctaa
    10201 gtttggtata aattcatttg catcagtagc tcctatagaa cacatagata ctattattac
    10261 ctctgctagt atagaccata atataataat agagttttct caaaaaggaa tacctttagt
    10321 catagctgat aattagcttg tatgattgat ttttagttcc ttgatgtaat aaattaaaat
    10381 taaggagcca aataattatt tatttagctc cacttatgtc taaactatac tttatatttt
    10441 aattatgtaa ctactttcag ttagcttatc atttttagtt atttgattgc cattttattt
    10501 aatttatcag ttttggttat tcgattattt tttaattgtc tgtcttttta atttattttt
    10561 agttatttaa actcctactt taaaataact taaatttgac ttaagtattg tttattctac
    10621 actgcatctt tcttcatttt aatactttgt tcttcttcca aagttaaatc ttttgtcata
    10681 agtatagcaa gtgcaattgc caatactcct ccaaccaatt ttccaattac aacaggtgta
    10741 atcatctctg gaactactcc tgctgtaaat cctaaatggt cccccaaagc tgctgtagca
    10801 caaaccagcc aagcagtatt tactactttc ccctttggac tcatatcttt catcatttta
    10861 tatactggaa tactattggc taaagtaaaa actaatcctg ctgcactagt ggcatccata
    10921 cctaattttc cacccactat tgtaagtggt ttattaagaa ctttaactaa taaatgtaat
    10981 attggaaaag tacctaataa cactattgca atacttccaa ttacttgaat accttctctt
    11041 ataggtgtca tgccttttat aagtactata cctgttattt cttgaaatgc tgctgctccc
    11101 aaacctatcg ttatcaatat agatataaat tttccaaaaa ctaatgaccc atttatcata
    11161 agtgtagaat taaattttaa acctactgcc aatattactg ccaatattat cactggtatc
    11221 atatttataa ttaccattga taagttaaat cctgctatta atcctcctat aatacctcca
    11281 aaaggtattg ttatcaagcc tatcagtaac ccttttgcaa agaatggtct gtcttcatat
    11341 tctataagac ctagtcctac tggaattgaa aatactaatg tacatccaag catggatgac
    11401 acgactaatc ctgcaagcat tcctgcctct ttattgtgtg caagttccat agccaatgga
    11461 taccctccca tatcattcgc taaaatagtt gcaaacatag atgggtcagc tccacagaga
    11521 ttgaatattg gtattattat aggacctagt acatctgaaa ttactggagc taaacaaact
    11581 ataccaacca ttcctaaagc caatggaccc attgaattaa acccttcttc aaactgctct
    11641 ccaagtccaa atttatttcc aattattcta tctaacgctc ctaaaagcac tcccacagcc
    11701 attatgtata atataatttt atcaaagggc atttttaatt cctcctatac atgtaaaata
    11761 ctctattgta acttaattag cacacacctt tttggtatcg ttttcccaat tcatagctct
    11821 ttttactgct tgattccatc caagtaatat ttcttctctt ttttgcttag tcatatttgg
    11881 aatatatttt ttgcttattg taaattcact tctaatttca tcaatactgt tccaaacacc
    11941 acaggaaagc cctgccatat accctacacc aagtactgtc gtttcaggat tttctgcaat
    12001 ttcaattggt atatctaata tgtcagattg aaattgcatt gtaaagttat ttttagtaat
    12061 tccaccatct actctcaaca tttttatttc tttccctgaa gccaaattca tggaatcaac
    12121 aacatccttg acttgaagtg caacagactc aagagtagcc cttactatat gttctttttt
    12181 agtacttcct gttatcccaa ccattgtgcc tctagcatat tggtcccaat aaggtgctgc
    12241 tatacctgca aatgctggta caaaatatac ccctccagta cttgatatgg aatttgccat
    12301 gtcatcagtt tctgaataat tttttataac tccaattcca tctctaagcc attgtattgc
    12361 agcacctgct atatacactc caccatcata agcatatgta actttatctc ctatcttcca
    12421 agcaactgtt gtcaaaatac cattacctgc tatatttaac tcctcaccta tattcatata
    12481 taagaaacat cctgtaccat aagtattttt aaccattcct ggttcaaaac atccattgcc
    12541 aaataaagct gctggttggt ctaccattga agcagtaatt tttatagaaa ccccacaaaa
    12601 tgcctcctta tctgtagttc caaaatcacc actagtaggc attatatctg ctaaaatact
    12661 tctaggcact cctataatgt caagcaactc tctatcccaa tccatatctc ttatattaaa
    12721 aagcattgtt cttgctgctg tagatacatc agttaaaaaa gattttcctc ctgtcatttt
    12781 ccatattaac caactatcca atgttcctgc aagtacttct ccatttttaa ttttttcttt
    12841 tacacctttt acattatcta ttacccattt aatttttgta cctgagaaat atgggtctat
    12901 aattaaccca gtcttatcta ttatcttatt ttctaaacct ggtatattct ttaaattttc
    12961 cacataatca taagttctcc tacactgcca tacgatagca ttgtaaagtg gtttacctgt
    13021 atttttatcc cataacatta ctgtttcacc ttgattatct ataccaattc cttttatatc
    13081 actttcattg attccacttt caaaagcctg ttgtaataca ttattactca cagtttttgt
    13141 attattccaa atttctatag ggtcatgttc aacatatcca gattgtggat atatttgagt
    13201 atgttccata taatcgctaa aaacaaactc tgatttttcg tttatcaata ctgctcttgt
    13261 tcctgttgtt ccttggtcaa ttgctaatac atatttcact taaaatgtcc ccctttattt
    13321 tcagaattaa taaattcttt tttatttttt gaatatataa tctattcaag ttatttatat
    13381 tttatatcat catgtggatt tatgcaatat tcattacaat attttttgtt atttttaatt
    13441 atggtgatta aaaaagtatt tttattgaaa tattattgca atattcatta ttttattcta
    13501 taatctagtt aaaataacac acaatatttt aatatttaca catagtcagt cattcgaaaa
    13561 tttaaattta actttttaag gaggctttac aatgaaaaaa acatatctat tcagtcctgg
    13621 accagtaatg gttacagata aagtcagaaa ttcacttctt cactatgata tatgccatag
    13681 aggaaacgag tttatggatt tattcaaaga tacacaacaa aaaatcaata aactatataa
    13741 tgcaacttct aattactact cattaattgt atctggctct ggaacatctg taaatgaatg
    13801 tgtactatct tctatctttg acaaagaaga tgcagttctg ctagttagta atggtgtttt
    13861 tggagaaagg ctagaagaaa tattatttac ttataatgta aaaacatata agcctaattt
    13921 tgaatgggca gaatacccag acctggacat tttagaaaaa tatataattg aaaattctga
    13981 tataaaagta attgctatgg tgttccatga aactagttca ggaatgataa atccagtccc
    14041 tgaactagga aaacttgcta agaagtataa caaaatattt tttgtagatg caataagtgc
    14101 ctcagcaggt gaatatattg atgtagaaga gtttaatata gatataataa caggtgttgg
    14161 tggtaaagct cttggagcat ttccaggttc tgcatacctt tgtgctaaag agagtatttt
    14221 acaaaatatt aaagaaaatc aatgtaagaa tgtatatctt aatctttata aacactataa
    14281 agttgctaaa tcatcttctc aaacaccaaa tacacctaat gttactttaa tatttgccct
    14341 taatgaagct ttaactgagt ttttggaaga tgacagtaaa atagaaagat acaaagaatg
    14401 ttctgctatt ttaaggaatg gcatggaaga attggggcta acatttttac ttccagataa
    14461 gtatatgtct aatacagtaa catctgtatt tctacctaaa gaaatcaatg taaatgagtt
    14521 tatattaggc ctagaaaggg aaagtggcta tgttgtctat ccaggaaaag gcaaattcct
    14581 agatgctaac atattccaag ttgcaaatat gggagaaata tatccagaag attgttataa
    14641 attcttagat atcttaaaat caaaattgga gataaaaaaa gttatataac agtttgtttg
    14701 taaaatccta caatacatca gtataaatta aatagactga tttttttaaa ttttcctata
    14761 ataaattata ttttatattt ttgtataagg gtgaactata atatgtaaag ttcaccccta
    14821 tttcaaatct ttttccttca gtataatctt tttcttttaa tttattttat atttttgaca
    14881 taattattct tacatttaat tgttttttat ttttgacgca atcgtttttt attca
//
LOCUS       DAMJVX010000086        14866 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMJVX010000086 DAMJVX010000000
VERSION     DAMJVX010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14866
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.090"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            550..1857
                     /locus_tag="PC407_002290"
     CDS             550..1857
                     /locus_tag="PC407_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497733.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLVLDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQSKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1943..2205
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2331..3134
                     /locus_tag="PC407_002291"
     CDS             2331..3134
                     /locus_tag="PC407_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2497734.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINKTLKEMKKDGTFDKIYDKWFK"
     gene            3172..3783
                     /locus_tag="PC407_002292"
     CDS             3172..3783
                     /locus_tag="PC407_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2497735.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3815..4486
                     /locus_tag="PC407_002293"
     CDS             3815..4486
                     /locus_tag="PC407_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2497736.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4785..5147
                     /locus_tag="PC407_002294"
     CDS             4785..5147
                     /locus_tag="PC407_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDF2497737.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            5583..6284
                     /locus_tag="PC407_002295"
     CDS             5583..6284
                     /locus_tag="PC407_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HDF2497738.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIINIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            6555..7373
                     /gene="larE"
                     /locus_tag="PC407_002296"
     CDS             6555..7373
                     /gene="larE"
                     /locus_tag="PC407_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HDF2497739.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNMDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKILGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLFNLGFSQFRVRM
                     HGEIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            7428..8576
                     /locus_tag="PC407_002297"
     CDS             7428..8576
                     /locus_tag="PC407_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDF2497740.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            8712..10100
                     /locus_tag="PC407_002298"
     CDS             8712..10100
                     /locus_tag="PC407_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497741.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYTTLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            10129..10827
                     /locus_tag="PC407_002299"
     CDS             10129..10827
                     /locus_tag="PC407_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2497742.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     ENDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            10830..12263
                     /locus_tag="PC407_002300"
     CDS             10830..12263
                     /locus_tag="PC407_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2497743.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            12285..13022
                     /gene="truA"
                     /locus_tag="PC407_002301"
     CDS             12285..13022
                     /gene="truA"
                     /locus_tag="PC407_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2497744.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    13133..13340
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13593..14792
                     /locus_tag="PC407_002302"
     CDS             13593..14792
                     /locus_tag="PC407_002302"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDF2497745.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
BASE COUNT         6047 a         1367 c         2643 g         4809 t
ORIGIN      
        1 tgtataaatt aattatgaaa aatttatata agtaaaatat attaacataa aggtaaaatt
       61 ttgtatacta aaagggaata tttacagttt aatggcagtt ttaaaataat aaattaaagc
      121 tgtcattttt atttaaaaat ttttagaaaa agtacaatag tgttaatgtg tgaattagat
      181 aaggtgtata taataattta gatgatatga tataaaatat gttgtaaatt aaatgttttc
      241 tttgacaata tatacacaag gatgataata tctatatgaa taaaagatag tgtgtaattt
      301 gataatatgt aattataaca ttatgatata attacaataa agtatgtaat aatctgtaga
      361 aatttcaagc tagttatcta agttttcgga taactaaatc taaacggaat acataatgta
      421 ttaaataaat gatgatacta aaatgatact atttttatag aaatttattg aggtatataa
      481 ttgatgaaaa taatatatta aattatataa tatattgagt tttacataaa agcgaaggtg
      541 ggaataaaaa tgaaagaaat tgctgatgct ataacatcag agtatttaag ctatggtatc
      601 aatgaagata ttagtatcta tgaaggtaga ttttgcatat atttagataa aaaatataga
      661 tgtaatggga aaatttatta taaaatgaca cctccaatat ctataagttt taaagcagac
      721 ataggttgtg ttgaagagat agacaatgaa gatgacaatt tagttttgga ttatgataat
      781 gctattcttg aagttcatgg atacaaaatt atttctataa caataaatac tttaagtgaa
      841 tttagtgtag aaggttatat caatgatgac tgcataaaat ctaagaactc ctatgttgaa
      901 tatgtagatt ttaatataat aaacttagat aaaatacctg gaaaactaat taaatacaat
      961 gataaggtat acgcaggtag aattgaattt gatataaatg attatgttgt aactatagac
     1021 aagcgatatg attatagaaa agaattaaaa tcagaattaa agtctaaatc tggagcaata
     1081 ataactcata taggtaggat tagaagaaaa gatggtagaa tttttagaac aaataataca
     1141 ataaatcttt tagatagaat atcaacagct ttgagtttta tgtgtggaag atatgtaggg
     1201 ttttgtttgg ccaaagggta tagaagtggt aatgaagttt atagaatatg gaatgaaaat
     1261 caaatatctc cttttagata tgtaccaaca tggtcagata cactttcaaa ttatcataat
     1321 atggaaaaat atataagcct tatgtgtaaa aagctagaag atttttatta tggttctgct
     1381 ataaagagtg ttgttgattg gtacatagaa tctcttggaa gtgctaccat ggaaaacaat
     1441 ataatatctg tacaaattgc acttgaaact ttgtcatatg taattctagt ggaacaaagt
     1501 aagatactta cagatgaagc atttgactgt aatttggcat ctaaaaatat aagactctta
     1561 cttgatacat gtaagatacc atatggtaaa catgaattaa atatatttga taatataata
     1621 aaaaataaat ttgatgatgg agtggattta gttatttatt taagaaatag catagttcat
     1681 ccaagtagaa agactcatag agctgtgtta gaggttgaag atatttggaa tataatttca
     1741 ataggaacga gatatattga acttgtttta ctctttattt taggttatag aggagaatat
     1801 tcaaacagat tagtagaaag gtgttatggt gaagtggaag ttgtaccttg gaactagatt
     1861 tttatattta ttttaaaata tatattgaca tatattgcct tatgctataa aatcaatatc
     1921 ataataataa ttcagaataa cattaaatgc taagaaaaga aatagtaaag gtagttaatc
     1981 ttaaacagag agtttccgat tggtgagagg agcaatagat tttactttga acacatcttt
     2041 gagcaggact ctgaaagagt agaaatactt tattaggaga tttccggaat ccttgcaccc
     2101 gttatagtgc tagagtataa taattttatt gtacttaaag aggttagtgt cgtgaggcat
     2161 taatgaacag aggtggaacc acgagagtta actcccgtcc tctagcagaa gctagaggcg
     2221 ggagtttttt aatataattt tttaagcgcg cagagaatta tattgcaata gatacacttt
     2281 ttaaaaaata atcaaaatat tttaaaaatt aaaaggcgag ggagaatatt atgaaaaata
     2341 tattaaaaaa agtaggaata tttacaatta tgttaggttt attaggtgga gttgtaggtt
     2401 gttcaaaacc agataatgaa aaagataagg atgcttcaaa ggaatctaaa aaagaagtag
     2461 ttgtagggtt tgataatacg tttgtaccaa tgggattttt agatgaaaag ggagatacag
     2521 taggatttga cgtagactta gctaaagaaa catttaaaag acttggaatg gaagttaagt
     2581 ttcagcctat agattggtca atgaaggaaa ctgaattaaa tgattcaaaa actgtagatg
     2641 tattatggaa tggatattct ataacagacg aaagaaagaa aatagtatca tatacagaac
     2701 catatttaca aaataaacag ataatagtta ctctaagtga ttcaaaaata aattcaaaag
     2761 ctgatttaaa agataaagaa gttggaacac aacaaggttc aacagcactt gatgcagtgg
     2821 aaaaggataa agattttatg aatagcttaa aaggtggagc accagttctt tatgatactt
     2881 atgataaagc acttagagat ttagagatag gtagaactag tgcagttgtt ggtgatgaag
     2941 ttttaattag atattatatg ggacaaaagg gtgaagacaa atacaaggta ttaaaagacg
     3001 attttggatt agaagattat gttgtagcaa cttctaaaga aaatccagaa ctttgtgaga
     3061 aaataaataa aacattaaaa gaaatgaaaa aagatggaac ttttgacaaa atttatgata
     3121 agtggtttaa atagaagata agttagctgt taagaagggg gcactaaagg catgttacaa
     3181 ggtttagaga tagttatagc aatgttttgt ataacattga tagtatcaat tccattgggg
     3241 attggagttg catttttaag attatcaaag aataaattag taagtggtat tacacaatgt
     3301 tatatattga taatgagagg tacaccttta cttttgcaaa tgatagtcat attttacgga
     3361 ttacctcttt taggaattgt atttgacaga tttacagctg gagtagtagc tttcttctta
     3421 aattatgcag catattttgc agaaatattt agaggtggaa tacagtcaat cgatagagga
     3481 caatatgaag cttcaaaagt actaggattt gataaattta ctatgtataa gagagtaatc
     3541 tttccacaag tttttaaaag aatactagca cctatttcaa atgaagtaat cacattagta
     3601 aaagatacat ctttagtata tatattagga ttaaatgata ttttaagaat atctcaaata
     3661 gcaatgaaca gggaagcaag tctgttacca ttatttgagg caggagcgat atatcttata
     3721 tttgtagcta tacttactaa gggatttgaa ttacttgaaa aaaaatattc ttattataga
     3781 taaagttaca atattatagg aggaggtttt ttatatgttg aaaatcaaaa acttaaataa
     3841 atcatttaaa aaaaatagag tacttaaaga tatttctttt gagttagaag aaggtcagat
     3901 tggagtttta cttggtaagt ctggtgcagg aaagaccaca atattaagat gtataaatgg
     3961 tcttgaagaa tttgatagtg gagagattat aatagataat gaggtaataa aaaataagag
     4021 agatatggca aaaatacgag gaaaaatagg tatggttttt caaaatttca acttatttcc
     4081 tcatatgaca gtccttgaaa atataattga atccccagtt aatgttttta aagttcctag
     4141 aaaagaggct gaagagaggg ctagagaact tttaagatta gttgatttag aagataaatt
     4201 aaattcttat ccatttgaat tgtcaggagg acagcaacag agagttgcta tagctaggtc
     4261 atgtgcattg atgccaaaag tcctttgttt tgatgaacct acttctgcac ttgatattga
     4321 tactattcaa agagtagtca atataatgaa tagacttaaa gacaaaggta tgacaatatt
     4381 aataattaca catgatgttg tcttctcaaa taatgttgca gataaaatta taagtataaa
     4441 agatggtata gtagaaaatg ttcaaataaa agaaaaaatt gtttagagtt ttataaatat
     4501 aaactagggt agaattttat aatataaata aagggcaagt aaaatcaaat atactatgcc
     4561 cttttgttat ctcaaaattt aatcttatat tttatgttta agttacatta taatttataa
     4621 taaaaataaa aggactattt aaatatataa tgtgaataaa tgtttaataa tttgaaatat
     4681 gataattttt tatgttgaaa gcttataatg attatgatat aaattggtta tagtatacaa
     4741 tttatgggta taacaataaa aaattagaaa ggaattttta taaaatgatt aaattatatg
     4801 gatatacaaa atgctctact gttaaaaaag ctaaaaattg gcttaaggaa aataatttag
     4861 aatttgaaga tatagatatg gttcaaaacc ctcctagtaa agaggagtta aaatcaattt
     4921 ataagactag tggatatgat ataaagaaat tttttaatac aagtggtatg aaatatagag
     4981 aattgggtct taaagatatt gtaaaaactg aatctgatga taaactttta gaaatattgg
     5041 taagtgatgg tatgcttatt aaaagaccat tacttttgga tggtaaaaat gtattattgg
     5101 gctttaaaga agatgtatgg aaaagtactt tgctaaaaga agattaggaa gagataggtg
     5161 tgttgattaa aattattaat taatacacct ataaatttat gtattaaatc taatctttat
     5221 aaatattaat tagcaaattc aaatcaaaaa aattcatatc aacaaatata ttacttctta
     5281 ttacaaatta tatcatatgt tttatattaa aatattataa tattacaacg aattgttaca
     5341 aaattactta ttattaacca tgaacatttg ttttgtgcta tcatatttat ataagggaat
     5401 tgtcataaaa aatatcttgt tcatacagtt ttatatagaa aaaagaaatg aatcttttct
     5461 ttatataagt atattttgta aattgcctct atatttatag tttaaataat agtggtttta
     5521 aaaatctgac aacaaaaata aaactttcaa aaaattaaat ctaaaaagaa taggagataa
     5581 atatgagtat tgaagattta tatgattttg aatgtgttcc tttgaacaat gagattgata
     5641 ttgatgtttt atttgaaaag atgatagaag atggaaaaga aaaagaatat acaccaatta
     5701 tagtcattga tgaaaaagta gggctattaa gaaaaaatat agatatgatt actaaagagt
     5761 atggttctct tgaaaactat agagagtatt gtttaacaaa atataatgaa attgatgtaa
     5821 atcaattttt tgattttagg aagagtgaga tttcagaatt attaaaggag ctaattgatt
     5881 tagaagatga tttttatgat tataaacatt ttagaccaat tataaatata gatatatttc
     5941 atgaatataa caaagtttac atagctaaaa ttccaacaat taaaccatat gaagtatttg
     6001 catacatacc tattggagga tttaatgatt gtcctagaga tgaagaacat atagctattg
     6061 caaaatattg gtatgaaaag catggaatct ttccaatcgc aattggatgt gatacagttc
     6121 aatattctgt aaaaaattta actaaagatg gtaaaaaatt tgatgactta tgtattgaat
     6181 tagtatttta ttgtgaggat attattatac aagggtatga aaccttaaag gctcttaagg
     6241 atgtattaaa acgctctaca atagtgcttt tttggtggga ttgatattct atcatttaga
     6301 tagtattaaa ttgttagtca ccaagaatcc acttacttta gttatgggga gtgtgtcaaa
     6361 ctaggttttt atacacagtg tagcatagta atatgtttaa attacaaagg ttaaaaaaat
     6421 actttaatat ttttaatatt ataattaata gatttaaagg taattataat ttataaaata
     6481 aatactttat atgttataat cattaagaat gtaaaaaaat aatatatagt tttatagttc
     6541 atatttttcc agatatggag gtaaattttg tgaaggaaaa agaaaaatta gataaattaa
     6601 aaaaaatgtt acttgaatta ggcagtgttg tagttgctta ttctggtgga gtagatagta
     6661 attttttgct taaggtcgca aaagatacat taggtgaaaa tgttatagct gtgaccattc
     6721 atgcaatgat gcattcaagt agggaaattg aagaggctaa acaatatact caaaattttg
     6781 gcgttaaaca tatcatatta aatatagaaa actttgattt aaaagaattt aaagaaaatg
     6841 gtgttgatag atgttatcat tgtaaaaaat atatattctc taaaataaaa gaggtagcaa
     6901 aagaacataa tataaagtac atagtagatg ggacaaatat ggatgattta ggagactata
     6961 gacctgggct taaggcattg agtgaattag gagtgattag tccattaaaa gatagtagtt
     7021 taaaaaaaga agaaattagg tcgctttcaa aaatattagg attaaatact tttaataaac
     7081 catcatttgc atgtcttgca agtaggattc cttatggagt tgagataaca gatgaaaatt
     7141 taagaatagt agaaaagagt gaagaatatc tattcaattt aggtttttct caatttagag
     7201 tgagaatgca tggagagata gctagaatag aagttgggca ggaagaacta ggtaaatttt
     7261 ttgaaaataa caattttaat aaagttgata ctaaattaaa aatatttggc tttaaatatg
     7321 ttacactaga tatgtcagga tataaaatgg gaagtatgaa tttaaatgtg tagattattt
     7381 taaaaataaa taagttttat agttaatata aggaaacagg aggttttatg aaaaattata
     7441 cattaatatc gccatgtttt tttggaatgg aaaaaatgct ggctagagag attactaatt
     7501 taggatatga aataataaaa acagaagatg gaagaataac atataaaact gatgagtttg
     7561 gaatagcaaa gtcaaacatg tggttgagat gtgctgaaag agtacatctt aaaattgctg
     7621 aatttgaggc aaaaagcttt gatgaattat ttgaaaatac aaaaagaata aactggtcta
     7681 gatatatacc ttatggagct caatttccta tatcaaaagc ttcctctata aaatcaaaat
     7741 tatacagtac accagatgta caagccatag taaaaaaggc tatagtagaa agcttaaaga
     7801 aaagttattt agaggatggt ttgcttaaag aagataagga gaaataccct atttttgtat
     7861 ttatacacaa agataaagta accatttcta tagatacaac aggagatgct ttgcataaga
     7921 gaggatatag agaaaaggca aataaagctc caataagaga gactttagct gctgggctta
     7981 tttatttaac tccctggaaa gcaggtagag ttttagtaga ccctatgtgt ggttctggta
     8041 ctatattgat tgaagctgct atgattggta taaatatggc acctggctta aatagagagt
     8101 ttatatcaga aaagtggaga actcttgata agaaaatatg gtgggatgta agaaaagatg
     8161 cttttaataa gatagataat gaatctaaat ttaagattta tggatatgat atagatgaag
     8221 aatctataga tatagctaga gaaaatgctg aaatagcagg tgtagatgaa tatatagagt
     8281 ttaatgtagg agatgcaact caatttaaaa gtgaagatga gtttggattt ataatcacta
     8341 atcctcctta cggagaaaga ttagaagata aagacagtgt taaacagttg tataaggagc
     8401 ttggttatgc atttagaaaa ttaaaaaatt ggtcgtatta tttgataact tcttatgaag
     8461 actttgaata tgagtttggg caaaaagcag acaaaaaaag aaaattgtat aatggtatgt
     8521 taaaaactaa ctttttccaa tatccgggtc ctaagcctcc tagaaataat aaataagttt
     8581 tacatgattt atttgtaaaa tataaaattt tattaaaata agtaaaacat tatgatgcct
     8641 tgaaacgtct aattattgtg aggagatttt tgtgaacaaa tttaaagatt tataggatta
     8701 ggaggagtta aatgaaagtg ttaaagctta ttactgtagt agccctgatg atgttatttg
     8761 ttactggatg taatataaaa actgaatctc cagaacaatt agcgaagata cctgactatg
     8821 atgatacaaa aaaagtattg tatgatggta ttgaccagtt actaaaacca gattcatcta
     8881 tgatattgcc ttctaataca aaagaagtag gaaaaataaa taaggtagat ttaaacagcg
     8941 atggaattga tgaattagta gtatttgaac aaaaagaaga tttaagtaat aatgtgagtc
     9001 aggtagggtt tgtaaccttg agttacaatg gagaaaaata cgtacttgga gaccattttt
     9061 tagaaaatgg cgaatcaata gaatatgcta atttttatga tttggactca gatggatata
     9121 aggaagttat cttattggtt aaatccaaag acaaaacgaa tatgcatata tataaagtag
     9181 tagataatga aataacaaaa atatatgact tggatgcatc atggcttcaa aataaagaag
     9241 attttaatga catgaaagta aaaattggat atatagatgg agatgacaag ttagacatat
     9301 tgatgcttca tttgaataat aagactaatg aaatgtttgc aagtgttgca aattttgatg
     9361 ggaaaatgaa aatcaaagat tctgttaagt ttgaaaatgt aaaaaactta agtgaattat
     9421 atacaacatt aggtaatgtt gcttcatctg ttggaaattc ttctgcagct ataaaaggta
     9481 ttgtacttga tattcctata ttgaaagata ataactatat tacgcagata ctttatatga
     9541 aagatgggaa aattaacaaa gcttttagtg attatgataa aactattact aaatcttatt
     9601 atatacctgt agatgatatt gacaaagata agattataga aatacctata gttgcaggca
     9661 gtacaggtaa taataaaaat acttatagtt ctaaatcatc tgcaactata agttggtata
     9721 gatggaatgg aaaagaaggt gctgactcaa gtttaatatt tacaagtcag atatattata
     9781 attataaata taattttaaa ttatttattc caaataattt atttgataaa atacttgttg
     9841 aacaggaatt tgttggagaa aaagcagtat ttaaatttta ctattttgac tattcagatc
     9901 gtaagaattt atttaatata gtagtagaga gtaaaaacaa acttgaagat agaaaaaata
     9961 caggtactca aaactctatt gtacttcaag agagcgatga atatactttc ttactagttg
    10021 taaacaatgc aaatgaaatg gataagctag atatgactat agacgcatta aaagaatatt
    10081 tctcattaat atatggttaa tattatactc aattggaggt tttattttat gaaggaaaag
    10141 atacttatac tagaagatga aattggtatt agaagttttg ttagtataaa cttaaaaaga
    10201 gaaggctatg aaatagttga ggcaggtact ggaagagaag ctatagaaaa aatgacaaca
    10261 gaaaatgata ttacaatagc tcttttagat gtgatgcttc cagatataag tggtatagaa
    10321 gtatgtaaat ttataaggga gaattttgac caagtaggta taataatgct tactgctaaa
    10381 gctcaagagg atgataaaat agaaggattt atttcaggag cagatgatta catcataaaa
    10441 ccatttagta taaaagagct tttagtcagg gtttctgctc ttcttagaag agtagcaaag
    10501 gatgattcaa gtgttaagtc gagtgaaata gtttctccac catttatact tgatatagac
    10561 aagagaaaat tatttaaaaa tggaaaggaa attgagctta caccaactga attttcaata
    10621 gtgaaatatc taatatcaaa tgctaagcaa tcgttaagta gagaccagat attagacgaa
    10681 gtatggggaa ctaactattt atatgatttt aaaatagtag atgtaaatat aagaaggata
    10741 agaaataaaa tagaagatga cccatcaaag ccaaaatata tacaaactat ttggggttat
    10801 ggatactgtt ttagaaagga agaataatag tgttacattt tgaaggtctg agaaaaaaag
    10861 taataaaaaa ctattttata ataattatta taatggttac tctgtttgaa ggattgttca
    10921 tgttttacat acaaaactac tattatgatt ctgtcaaaca actacttgaa tctgaaataa
    10981 aatatgctga tgaatataat gctattacaa tggaaactac aagttttgag aaaaaagtaa
    11041 aaaatatatt tgataaacaa ccattaacta aaaattcaga atttggaatt tcaataatag
    11101 ataaagataa aaatattata ttagaccaat atggttttaa aagtaaagaa aaagcaaact
    11161 atgaagatgt taacaatgca ttaaaagata taaaaactaa aaatttaaca ccttatactt
    11221 atagaatacc agatacaggg gagcatgtta tgagtatctc cttaccactt aaggtaaata
    11281 atataataga aggtgttgtt agatatacag tatcattaga tgctattgat aatgcaatat
    11341 taaagcaagc tacatggctt atattagcag gaatatttat acttattata gctatactaa
    11401 taagtcttaa atttgctgag actcttataa aaccattacg agagctaaag aaatttgcaa
    11461 atgaattggc tgtaggaaat tataatataa aattagaaaa gatgaagatt gtagatgatg
    11521 agattggtga tttagcacag acatttgaac acatggcaca tgagattgac aaaagtgaaa
    11581 aattaaaaga ggaatttata tcatctgtat ctcatgaact aaggactcct cttacatcta
    11641 taaaggggtg gagtgaaacc ttaggttatg aaagtataac aagagaagaa ctagacttag
    11701 gtttaggcat tatacaagat gaaacagaaa gacttataaa actagttgaa gaattattag
    11761 acttttcaag gctttcttca gacaggataa aacttcatgt tgatatagtt gatgttgaag
    11821 gtttaattgt aggtgttgta aatcaactta aagttaaagc agctgaaaaa gatatatcac
    11881 ttttatttga atttgaaaat gaatttatag agaacattca aggagataaa aatagattaa
    11941 gacaggttct cataaattta atacaaaatt catttaaatt tacgagtcaa ggtggttaca
    12001 taaaagttgt agcttctcaa gatgaagaga taacaaccat atcagtagag gataatggtt
    12061 caggtataga aaagcaaaat ttaaataaag tgttagataa atttttccaa gaagattata
    12121 ataaggctgg tagtggatta ggattagcaa ttagtaatga aattgtaaaa cttcatggtg
    12181 gaagaatgaa aatagaaagt gaaaagaatg ttggaacaaa aataactttt aatataaaaa
    12241 ataaatttgc taaacaagca taaattctta gaaagaggat taagatgaga aacataaaaa
    12301 tgatagtagc atacgatggt tcaagatata aaggatatca aaaattaggt gacaataata
    12361 tgactattca agaaaaactt gaaaatgttt taagtaaaat gactaatgaa acagtggaga
    12421 taatagggtc tggaagaact gatatgggag ctcatgcaag aggacaagtg gtaaatttta
    12481 gaactaattg tatggattct ttggataaaa ttcaaaaata tttatatgaa tatttaccag
    12541 aagatatagt tgtaaaaact gttgaagaag ttgatgaaag atttcattca agatacaatg
    12601 taaaatcaaa gacttatatg tataaaattg acaataataa gtatcacaat ccttttataa
    12661 gaaaatatgc tactcatgta agtaaaaaat tagatttaga tagaatgaga aaagcaagtg
    12721 agtatttagt aggagaacat gattttacta gttttgcttc ttctaaatct aagaaaaaat
    12781 caaatgtaag agaaatttat tctataaata taaaagaaga tgataatgtt atagaaatat
    12841 atgtagaagg aaatggattt ttgtacaaca tggtaaggat aatagtaggt gctttgatag
    12901 atgttggtct aaaaagaaga gctccacaag atattaagta tatgcttgaa tcaaaagata
    12961 gatgtcaatc atcagatact gcaccagcta aaggactatg tctttggaaa gtaacatatt
    13021 agtcaattat ataaaactct ctttaaaata aaaataaaat aaattatata gttgacataa
    13081 atggaaaaat aaactattat atattttata agagagatat aaaactttat atttaaatcc
    13141 tgtgagaaag agagtaactt ctatgtgttt tatagagagc tgaggatggt ggaagctcag
    13201 caaataagta ttagtgaaaa gaactttgga ggatactaat tgaatttagt atgttagtag
    13261 gtaatctcgc cttaagagaa agagtggata agtttatctt atcaattaga gtggtaacgc
    13321 ggatatattt cgtctcttgg tttttccaag aggcgttttt taatatattt aattattagt
    13381 gtttatctgg tatgttgaac atttactaat tgattagcta agtttatcaa taagagtggt
    13441 accgcggata tttcgcctct tagtatttta aaatactaag gggttttttt agtattaaaa
    13501 tgattttatg tgagtctaaa ttctcagagt cattcaatat taagaatttc tataaaataa
    13561 aaattataat ttaagattgg gaggaattaa aaatgaagga aaaagtagta ttagcatatt
    13621 caggaggact tgacacatca atcataatac cttggttaaa ggaaaattat gaagatatag
    13681 atgttatagc tgtttgtgga aatgtaggtc aggaagataa aatggaagat gtatatgaaa
    13741 aagctttaca aagtggagca tcaaaggcat atgtggatga tataagtgaa gaatttgtaa
    13801 ctgaaactat atttaaagcc gtaaaagcag aggctaaata tgaaggaaaa tacttattag
    13861 gaacttcttt agcaagacct ataatagcta aaaaattagt tgaagttgct cataaagaag
    13921 gtgcaaaata tatatgccat ggatgtacag gaaaaggtaa tgaccaagtt agatttgaag
    13981 ctacaattgc agctcttgac ccgactataa aagttattgc accttggaga atttgggata
    14041 taaaatcaag agaagatgct atagactatg ctgaaaaaca taatataaag gtaacagcaa
    14101 ctaaagctaa aatatattca gtagatgcta acctttggca tgtttcaaca gaaggtggag
    14161 atatagagca cctagaaaat gaacataaaa aagatgttta taaacaatgt gtagacccag
    14221 aagatgcatg tgatgtagct gaatatgtag aagtttactt tgaaaaaggt gtacctaaaa
    14281 aagtaaatgg cgaagaatta tctccagttg cactgattca taagttaaat gagttgggtt
    14341 gtaaacatgg aattggtgtt atagatatag ttgagaatag acttgttggt atgaaatcaa
    14401 gaggtatata tgagactcca ggaggaacaa tcctttatga agcacataat attttagaaa
    14461 gtgctacttt agacaaagat acattacatt tcaaacaaat ggtatcttat aaatacggag
    14521 aacttattta caatggacta tggtattgta aattaagaga atctatagat gcatttatgg
    14581 aacaaactca agataatgtt acaggaacag taaaagttaa actttacaaa ggaaatataa
    14641 aacctgctgg aatatttact gaaaatgctc tttatgatga aggaatatct tcttttggaa
    14701 atagtgaact ttatgaccac aaagatgcag aaggatttat taatttattc actttaccat
    14761 taaaaatcag agctatgaaa gctggaaaat aataaaataa attatttaag agtgtataaa
    14821 aactattttt aggtttttat atgctctttt tttatttgaa tttatt
//
LOCUS       DAMJVX010000087        14855 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.091, whole genome shotgun sequence.
ACCESSION   DAMJVX010000087 DAMJVX010000000
VERSION     DAMJVX010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14855
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.091"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            143..1933
                     /locus_tag="PC407_002303"
     CDS             143..1933
                     /locus_tag="PC407_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDF2497746.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLDCGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            1951..3117
                     /gene="rpoD"
                     /locus_tag="PC407_002304"
     CDS             1951..3117
                     /gene="rpoD"
                     /locus_tag="PC407_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDF2497747.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            3282..3971
                     /locus_tag="PC407_002305"
     CDS             3282..3971
                     /locus_tag="PC407_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2497748.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            3985..5082
                     /locus_tag="PC407_002306"
     CDS             3985..5082
                     /locus_tag="PC407_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF2497749.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            5144..5716
                     /locus_tag="PC407_002307"
     CDS             5144..5716
                     /locus_tag="PC407_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2497750.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEVLEEMNIEVDIFRVSDEVAKT
                     LPQYYLINYKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(5776..6243)
                     /locus_tag="PC407_002308"
     CDS             complement(5776..6243)
                     /locus_tag="PC407_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2497751.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRI"
     gene            complement(6629..7105)
                     /locus_tag="PC407_002309"
     CDS             complement(6629..7105)
                     /locus_tag="PC407_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2497752.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLRVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(7225..8316)
                     /locus_tag="PC407_002310"
     CDS             complement(7225..8316)
                     /locus_tag="PC407_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDF2497753.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDVDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNKLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(8309..8698)
                     /locus_tag="PC407_002311"
     CDS             complement(8309..8698)
                     /locus_tag="PC407_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2497754.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            8890..9336
                     /locus_tag="PC407_002312"
     CDS             8890..9336
                     /locus_tag="PC407_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497755.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEDIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            9600..10325
                     /locus_tag="PC407_002313"
     CDS             9600..10325
                     /locus_tag="PC407_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2497756.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCKYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            10312..11556
                     /locus_tag="PC407_002314"
     CDS             10312..11556
                     /locus_tag="PC407_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2497757.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMKNIDYGQKILSKYNYDKSIKINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            11636..12313
                     /locus_tag="PC407_002315"
     CDS             11636..12313
                     /locus_tag="PC407_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2497758.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            12314..>14855
                     /locus_tag="PC407_002316"
     CDS             12314..>14855
                     /locus_tag="PC407_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2497759.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDQNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIKKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGESYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAIEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGFGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNDFNFYDEISSSESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDN"
BASE COUNT         6083 a         1529 c         2481 g         4762 t
ORIGIN      
        1 atataaaaaa taaaaatttt ttgattaata agaaggatat caataaaaaa agtcgaattg
       61 tgtatattga gtgattgaca aatttagaat atagtgtaga atagaaatgt taacacaaaa
      121 aattacacca gaaggtgata atatgaatga tataaaagac attattgaag aaataaaagc
      181 tagatgcgat atagcaagta taatatctga atatatgaat ataaaacaat ctggagctaa
      241 ttacaaaggg ttatgtccat ttcatggtga aaagacacct tctttttata taaatacatc
      301 aaaacagatt tataagtgtt ttggttgtgg tgaaggtgga gacgtaataa attttgtcat
      361 gaaaatggaa aatttggatt ttatggatgc tgtaaaaatt ttagcaaata aatgcggtat
      421 agaaattaac actaacatga atgaagaaac taggattaaa atagaaaaat ctaagaaatt
      481 tcaagatatc catacggaag cagctagatt ttatctttca aacttattgg gaagcaaaaa
      541 tcttgggtat gaatatttaa gaataagagg actagatgat aaaattatta agaaatttgg
      601 tttaggattt tctttggatt catggaattc acttatgaat actctaataa gtaaaggata
      661 caaaaaacaa gacttattgg attgcggtct aattgcaaaa aatagagatg gaactaattg
      721 ctatgataaa tttagaaata gagttatgtt tcctatattt gactatagag gaaacataat
      781 aggatttggg ggaagagtat tagatgattc tttgccaaaa tacttaaatt ctccagacac
      841 attaattttt aataaaaaac agaacttata cggattaaat tttgctagaa aaaatttaga
      901 aagtaaaact atagtcttag ttgaaggata tatggattta atttcgcttt atcagtatgg
      961 tataaaaaat gtagtagcaa cccttggaac agccttgact gaacaacaag gcattttaat
     1021 aaagagatat gcggacactg ctattatatc ttatgattct gatgaagcag gaataaaagc
     1081 gactttaaga gctatagata tacttactaa attaggtatc aatgtaaaag tgttagactt
     1141 aaaagatgct aaagacccag atgaatttgt aagaaaatac ggacttagtg attataaaaa
     1201 agctatggat gtttctactc actacataaa atataaaata gatcatctta aaaaagaatt
     1261 taacattcaa aaagatgaag aacgagtgaa gttcgcaaaa gaagcttcta aaattataaa
     1321 acaattgaca agtcctgttg aaattgattt ttatacaaag tatttaagca atcaaataga
     1381 tattaatgtc gagtctataa agagagaagt ttatggcaaa aattacaata agccatataa
     1441 taacaaaaat caaaagaaaa tagaagaaaa agtaattgaa aaagtcgaag tcagacaaga
     1501 cggaaaacaa ttagttgaag aaactctaat aaaaataatg ttagaagaca aaaaaattag
     1561 agaaatagcg ttacttaaag tagaagaaag tgatttttta ttgaaagaaa gtaaagaaat
     1621 tttaaattac atgattaaaa atcaagaatt ggacaaaata actattgaca aattgaaaag
     1681 tttaaacata tccgaagaat acttaaaaga attaaattca atttctttga atagtataaa
     1741 cttagagaac acaaaagaaa tagaaggaat aataacaaat atcagaaaaa actcattaga
     1801 agagcaaatt aatagcttgt tgagagaaca acaagaatta gaaaataata atgatatgaa
     1861 agaggtagat ggtagagtta tggaaattgc tttaaaaata gttgagataa ataaaatttt
     1921 aaagagcttg tagagaaagg aggtttgagt gtggaaaata aatcgaataa aaaagagtta
     1981 aaaaaggtta ctgctaagac attaatagaa aaaggaaaaa agcaaggttc tctgacactt
     2041 gcagagataa tggaagcttt ttcggagact gaacttgata aggatcaagt agaaaatctt
     2101 tatgagactt taggaaattt gggaatagaa ataacagaaa caaaaaatta taaagctgat
     2161 atagattttt cggttgctga tgacgattta agtataggcc acttagatga agatgcagag
     2221 gcaatttcac atgatgactc ttctgcaata gaaatagaaa ctgtggattt atctttacca
     2281 aaagggataa gtatagatga ccctgtaaga atgtacttaa aagaaatagg aaaaattcct
     2341 ctacttaaac cacatgaaga agtagaattt gctagaagga tgcacgaagg tgatgagata
     2401 gcaaaacaaa ggttagttga agcaaactta agactagttg taagtatagc aaaaagatat
     2461 gtaggaagag gtatgctttt cttggattta atacaagagg gaaatttagg tcttataaaa
     2521 gcagtcgaaa aatttgacta tacaaaagga tataagttta gtacttatgc aacatggtgg
     2581 ataagacaag ctataacacg tgctattgca gaccaagcta gaactataag aataccagtt
     2641 catatggtag agactataaa taagctaata agagtatcaa gacaactact tcaagaactt
     2701 ggaagagatc caaaaccaga agaaattgca aaagaaatgg aaatgacaga agataaagta
     2761 agagaaataa tgaaaatagc tcaagaccct gtgtctttag aaacaccaat aggagaagaa
     2821 gaagatagcc atttaggtga ttttattcca gatgatgatg ctccagcccc agcagaggca
     2881 gcagcatatt ctttattaaa agaacaaata gaagatgtac ttggttcgtt aaatgataga
     2941 gaacaaaaag tattaaagct tagatttggt cttgaggatg gtagagccag aactcttgag
     3001 gaagttggaa aagagtttga tgtaactaga gagagaataa gacagataga agcaaaagca
     3061 ctaagaaaac taagacatcc aagtagatca aaaaaactta gagattattt agactaaaaa
     3121 gatacatcat tgatgttttt gtgataaatg ccatgagata agtgatatct catggctatt
     3181 ttgtagtaaa gatttaattt tatttttgta gttatttcag tctaatatct ataacttgga
     3241 ttaaatccta taaataaagg gaattgaaag gagaattata tttgaaatta acagatagat
     3301 tattgaaaat agcatcatta gtgtcagatg gtaaaaaaat agctgatata gggacggacc
     3361 acggatacat accagtatat ttgttaaaag agggaaaagt accatttgca gtgctagcag
     3421 atgttaataa aggacctctt gacaatgcac ataaagaagt catacagaac aatcttttag
     3481 ataaagttga tttacgattg ggttctggta tagagatact tgaaataggg gaagtagaag
     3541 aagtgataat tgctggtatg ggtggaatct tgataagtga gcttctcgag gctaaaaaag
     3601 aagtagcaca taatgtagaa aaattaatat tacagccaat gcaagcacaa gaagaactta
     3661 gatattacct tttaaacaac ggatatgaaa ttttagaaga agttttagtg agagaagatt
     3721 ttagaatata tgagataatt gttgccaaat atacaggtaa aaatactata atagaagatg
     3781 agatatatta tgaggttgga ataaagcttt tagaaaacaa agattctctt tttaatgatt
     3841 ttatagaaaa aaagataaag acatatagtt ctatagttaa taaattagaa gggaaaaatg
     3901 gggaagctat agataagaag agaaaggaaa gcgaagttgc aataaaaaaa cttgaaaatt
     3961 taataaaata aataggaggt atatatgcta ttaaagagtc taactagaaa aattgaaaaa
     4021 aagtatcctc taaacttagc agaggattgg gataatgtag gtcttatagt tggagatttt
     4081 gatatggatg taaaaaaggt attggtatct ttagaagcta atgaagatgt tattgatgaa
     4141 gctatatcca aaaatataga tttaattgtg acacatcatc cttttatatt tgggaaaatt
     4201 aataaaatta atagtggaga tttaaaaggc agacttatcc aaaaacttat taaaaatgat
     4261 atatcacttt attctatgca tacgaatttt gatatagcat ttgatggtct aaatgactat
     4321 tttatggaaa ttatggaatt tggtaattca aaggttttag atataactaa aagcgaaaat
     4381 ttgtataaat tagcagtata cgttccacat aactattcag atgaacttag aaaggtttta
     4441 agtaattcag gagcaggtca tatagggaat tatagtgatt gtacgttttc aatagaaggt
     4501 gaaggacaat ttaagccatt agaaggctct aatccgtttc ttggaagtgt aaatgatata
     4561 gaaactgtaa atgaagttaa aattgaaact gtagtaccac agaagctttt aggtggggta
     4621 ataagttcta tgttagatgc acatccttat gaagaagttg catatgattt gtacaagtta
     4681 gaaaataaag gtgagatatt tggactaggt agaataagta aacttgataa aagtatgaca
     4741 ttagaaagtc tttcaaggaa aataaaagag aaattaaata tgaaacacat aagagtagtt
     4801 ggaaacctta gtacagacat aacgaaagta gccgttgtaa caggagctgg ttcagaattt
     4861 gtaaaaaaag ctaagagaca aggtgcagaa gtactaatta cgggtgatgt aaagtatcat
     4921 gaggcacaag atgcacttga tattggaatg tgtatagtgg attgtggtca ttttgataca
     4981 gaagatatat tcaaaaatgt tatgaagaga tttttagatg aatttagtga aattgaagta
     5041 ataaagagta atgtatattt gaatccattt agtactattt agaagtgttt gattaaaaaa
     5101 tactatataa aaaataaatt tgagtttaat tagggggata atgatgaaag tagcagtaat
     5161 ttttcatagt gtttgtggga gtacatattt attagctaga gaatataaag aagtgcttga
     5221 agaaatgaat atcgaggtag atatatttag agtaagtgac gaagttgcaa aaacacttcc
     5281 tcaatattat ttgataaatt acaaagaata caaagacgaa tttgaaagta taaatgtaat
     5341 taaatctgga aaagaaatat tagattatga tgctatattt atgggttcac caacatatta
     5401 tggaaatgta tctggaccaa tgaagatgtt tatggatagt ttttctgata tttgggtagg
     5461 tgctcctttg agtggtaaaa tatttggttg ttttgctaca gcaggttcac aacatggagg
     5521 aggagaatta gctttgcaag ctatgaatat atttgctcaa catatgggaa tgactctttt
     5581 gtcagttcca tgttcagtaa gaggaggtta tccagcgtat ggaatacttc atatagcagg
     5641 agataattct gatataagac caaatgatga tgccaagata ggtataagag attacctaaa
     5701 aagacttaac atataagaat atcatataaa aatacctaaa agtagctgta tatactttta
     5761 ggtattttta cttaattata tacgtgaagc tttaccagaa atatggtcaa tttctatttt
     5821 aacaacagtt gttaagttca tatccttctc tatgtacttc atgccttctt caaagaatcc
     5881 tttagaatat ttttttatta tttctacaag agcatttttc ttttcttcat tttctacagt
     5941 gcaagctttt ccaaaaacta tagcactaga ataagtagta ctaaatttat ctggaataac
     6001 actttcatta gcaactacta aaaaagaaac tttgttgttt ttcgatatat tatctaactt
     6061 atgtccattt cttgcacaat gaaagtaaat agaatcatta aagtatacat aatttacagc
     6121 aactccataa ggataaccat tttcagatat agtggaaaaa gtaccaaatt caccattttt
     6181 taatacttca acagtatctt cttttgtcat ttctcttttt ttcaatctca tttctttaaa
     6241 cataatatca tcctttctaa ttttgatgct taaattaaat tttagcacat ttataaagat
     6301 tatgctatgt tttttacaaa ttttaagaaa aaaatatagt ttaagataaa aggtagtgaa
     6361 agagaagcta tttggaggtt tatttatgat attattgtta gagtagtaca aacaatacta
     6421 ataaatttaa tagctggttt cttgttattt aaggtagtaa gcatgtctat aagaagggga
     6481 agttatcgag ggtagaagtt gttatttata acgatattat aaagaaaata ttattatata
     6541 aaataataaa tagtaaagag gtatcaagtt gaatataata cttaatacct cttcaaaatt
     6601 aaattataaa agtgttgtgg gaattgtcct atacatggta aggagataat aaagaatgta
     6661 tctttaaagt ctcaactaaa cagcaaaatt tatccaacaa acatacaata aatgcttctt
     6721 tgtatctagg tggcttaaca gtaagtggcc acatgtgttt taaaattatg tccgtttctt
     6781 tttcatttat ttgaaaaaat aaagttgcat tttttaatgc ttctctaggg tgagtaaatc
     6841 catgtaatcc ttttctatca cctttgtaat gccaatcata aagaaaaaag tcgtgtagta
     6901 aagctcctct aataacactt tgaatatcaa cacgtaagtg caatttttta cataatagat
     6961 aactataata tgctactgac aaactatgtt caagacatga aacattgcca tgatgaggaa
     7021 atgttctcat aagttgaact ttttcagaag aaagtatctc agaagcacat tcattaaaat
     7081 atttgatttc agcttttgac aacattatga aaatctccat tcttagtaat atataaatac
     7141 tatacttcaa cgataatatg aaatatttct tatcctacaa aatataatac ccaataatac
     7201 aagtaagata ccttttatgg tattttaaag taagagagat tttattaggc taaacaatga
     7261 aaaaattaaa agtaaagcta tagcaatata tagtattttt ccaagaggat tgccaaaatt
     7321 tatagtccat ccaataccaa accttttatt aaccattaat gatgggtcat ttggattatt
     7381 atacatgatg cctgctatcc aatacttctc atcttcttct ggagaataag atgagttgaa
     7441 ttttaaattt ggggaattaa tatatgttgc tattaaataa attataacta aaagcgttgt
     7501 aatattcata gcagttgata gttttgtttt tattgataaa agagtagtaa aaaactgtgt
     7561 tgtcattata atcatcaata ttaaaaacaa ataaccaatt ttatttaaat atttaatgtt
     7621 ttcaactcta ctttcttcaa ttctatttgt atctatctta atccttgact ttatggatcc
     7681 aatggaagtt atatataata aaaccattaa acaaaagtca gtaccgatta atttaaatac
     7741 attaagaaat gatttatcta taaaaacatc aggagaccca tttatgttcc aatgagtggc
     7801 aattgagtct ggcaatttat tataattaaa tgctataaaa atactcatcc ccaaagtaaa
     7861 tagtatcggt attaaataga ttattttaaa tttttttatt attttatttt tcttatttat
     7921 aaaatccata tctactatta attttgaatc tacatctata tgacctaatt tagaataaat
     7981 ttctgattta agcttcttag cttttttatg tgtatgaata tatagtatac tctcatataa
     8041 taaaaaacct ataataccaa taaaatatga aagttccaat ttattaaatg taaaaaccag
     8101 aataaaaact aatataaagt ctataataaa acctaaagat aatagttttt taaattcttt
     8161 atctaatttt ataaagtaat tataattttt atgctcttgg tctatagaaa ctccatagat
     8221 gtgtttttta cctacaaaat agggcataaa ataaaatgta gcatacatca aaattagaac
     8281 tggaaatatt gtaaatagac attcaatctt actcataata tttcacctca tacttgtcaa
     8341 ataaacttga acttagtgac aaaaattctt ctttactaat acctgataaa taagattttg
     8401 ccaccaataa ttccaagtca agtttatttt tttgacgagt tgtattatct attttcattg
     8461 gtagttgagc tacaatagca ccttttcttc tatcaatatt taagaatccc tcttctttta
     8521 actcattgta ggatttgttc actgtatgta aatttacacc aatattttct gccatacttc
     8581 ttacagatgg aagtgattca tttattttca attctcctga agctatagcc ttagttattt
     8641 cttctttaat ttgtacataa atagatttat cactattaaa atctaaattt aaaaccataa
     8701 aaaacctcca ttatgcttta taaatgaaaa cgattgttat atacatagta taacataaaa
     8761 aataataaca aacacaaaat ataagtttat attttgtaaa aattgtaatt taaaaaataa
     8821 cataattttt attaaaataa tacagatact atttatagat agaaataaaa attagaaagg
     8881 agaaaaaata tgttaacata tttaatgaaa agacaaatgc ataaaaaaga tgatggaatg
     8941 agtaaaccaa tgatgattac aggagctgta attacaggtg taggagcata tgctgtatac
     9001 aaaatggtaa aaagccataa atctaattct caaatggtca atacaaatta ttatggaaat
     9061 agcgattatg atgattataa atatgacgaa tttgattatg actgtgattg tagagacaca
     9121 aaacaagatt atgaatatga agaattaaaa aaaaaagtaa aagaatttaa ttcaagaaga
     9181 ataaattgtg aaaattgtcc acatgtttct caagaagaaa tgatgcaata tgtaaatagt
     9241 gttaaagatg taaaggaaga tattgattta catgaagatg ctaaaaaaga taatatttac
     9301 aaagaagatg aatacaaaaa atatgaagaa gaataagtaa tattttgtat aactgaacaa
     9361 atagtaatgt caaattattt aatatctagt tttaatatgt aagttgtaac ttaacttgaa
     9421 agaatcagta tcattcttta gatagttaag ttacaactta cattttgttt ttataaattt
     9481 taatagttct ttgtgactat tttgtaattt tagaaatatt ttagtatcca gggtaattgt
     9541 gtataataaa acttgtatag atagatagta tgaaattaag cagatagttt aaaattaaaa
     9601 tgaaaagaaa ggaaatagtt atgttaaagt tgttgatagt tgaagatgat agtactatat
     9661 cttttggaat aaagtatgct ttagaacaag aaggatttag tatagatata tcaaaggatt
     9721 tatctagtgg aaaagaaatg atttcttcta atgaatatag catgatactg ttggatgtta
     9781 cattgccaga tggaactggt tatgagcttt gtaaatatat aagaggattc tctcaggttc
     9841 ctataatatt cttgactgct tgtgatgaag aagtaaatat tgtaatgggt ctcgatatag
     9901 gtggagatga ttatataaca aaacctttta gaattagaga attaatatcc agaataaaag
     9961 ctgttttaag aagaaaaggt aatacttcag aagaaaataa gaaaatactt aaatttggag
    10021 atttaagtat atatacatta gaagctagag tatataaaaa tgataaagaa atatttctga
    10081 catcagttga atataaactt cttttaattc ttatacagaa taaaaatact gttttaagta
    10141 gaactcaaat attggaaaaa ctttgggatg taacttatga ttttgtaaac gacaatactc
    10201 taactgttta tataaaaagg cttagagaaa aaataggtga tgatttatgt gaaccaatct
    10261 acatagaaac tgtaaggggg attggttata aatggatagg aagtgaaaat agtgtttcta
    10321 tataatccag aagtgaagaa gtttttaagt aagtatgtta ctttgatgtt tattgttatc
    10381 ataatatcta ttggatttag cataatcaac gtgtctttaa ctaaagagat ggttgtaaga
    10441 aataatcaag ctataatagg gactttatcg agtaagtatc caaacttaga aagtcagata
    10501 gtagacataa taactcaagg gaagtctatg aagaacatag attatggtca gaaaatatta
    10561 agtaagtata attatgataa atctattaag ataaactctg agcctattat ttctaaatta
    10621 gttttagaga ctatcaaaat aaatataata ttagtatgta taatatttat tttgatattt
    10681 gtgttggttg ttagatattt tacgtcaatt tacaatgatt tatcagatat gactaagtat
    10741 gtttactata gttcagaggg taagccattt gatatgaaaa acaagaatca agaagggcag
    10801 ataggtcttc taaaaactga actcttaaag atgacgacaa ttcttaatga aaaggttgaa
    10861 cttttaaaaa gagaaaagat atttttaaac aatacaattt ccgatatatc acatcaatta
    10921 aaaactccta tgacttcttt aataatgttg aatgatttat tatataatga tgtaccatac
    10981 gaagtaaaaa tcgatttttt aaataagata aaaaatcaat taaatagaat ggattggttg
    11041 attaaaagca tgcttaaatt atcaaaggta gaagcaaaag tgataaattt taaaaaagat
    11101 aaggtaaaat ttagtgaact catacataga gcaatgctat ctatgaaaat acctatggag
    11161 attaaaaatc aaaaactgac tatagaggga aatgataatg tatcttatat tggtgatatt
    11221 gattggtctg tggaagcact agtgaatata atcaaaaatt gtgtagaaca tactcctgaa
    11281 tttggaaata taattataac ctataaagaa aatccattat tttctgaact aataataaaa
    11341 gatgagggtg aaggtataga taaaaaagat ataccacatg tatttaaacg attctataga
    11401 ggaagaagta gttcaaaaga agatagtgta ggaataggtc ttgcaatgtc aaaatctata
    11461 atagaaagcc aaaatggcga tatatatgta aacagcgaaa aaggtaaggg tacagaattt
    11521 catataatat ttcataaaat gtatgatagt gactaatctg taatatttaa ttcactttat
    11581 agtaatagcc aaaacctaat atatgaagta tagaatataa atattggagg tagttatgga
    11641 aattttaaga gtagaaaatc taactaaaag ttatggtaaa aatgaaacaa aagttgatgc
    11701 tataaaaaat gtaagtttat cagttgaaaa aggaacgttt atagcaataa caggtccaag
    11761 tggaagtggt aaaagtacct tattacattt attaggagga gttgacagac caactagtgg
    11821 taaggtttat ataaatgatg ttgatatata taatttaaaa actaaggatt tggcgatatt
    11881 tagaagaaga aatataggtc ttatatatca attttacaac ttaattccag tcttaaatgt
    11941 gaaggaaaac atattgcttc cagcagagct tgacaataga gatattgata gagaatattt
    12001 tgatgattta atgaaaacat taggtctaat tgatagagaa aagcaccttc caaaccaatt
    12061 atcaggaggt caacaacaga gaacctctat aggacgtgca ttaattaata gaccttctat
    12121 aatattagca gatgagccaa caggtaattt agatagtaaa aattcaaagg aaatattaga
    12181 attgttaaaa ttgtcagtaa aaaaatacaa tcagacacta ataatgataa ctcatgacaa
    12241 aaatatggca cttcaagctg atagaattat aagcatagaa gatggaatca taaaaagtga
    12301 tgaggtgatg tagatgaatt tatatacatc actaaccatt agatacttaa aacaaaataa
    12361 aagaagaacc attgtaacca taatcggtat aatactttca acagctttaa tttgtggtat
    12421 aggaaatata tttgaaagtt ttatggatta tcaaatgaga gaaactataa agaatgatgg
    12481 aagtttccat gttacatttt atgatgtaaa tagaaaaaat gtggaatatg taacgaaatc
    12541 agctgaaata gaaaaacatg catttaccaa acaacttgga tattctaaat tagaaaatag
    12601 tgaaaatgct atattaagta taaagcaata tgatcaaaat gcaataaacg gatataaagt
    12661 ttctataaaa gaaggtagat ttccagctaa ggaaggagaa attgttttaa gtgaaagtat
    12721 tattaatctt atagatgata agttaaaaat aggagataag ataactttaa aagtagggga
    12781 tatgtttgac tcaagcaaga aaaagattga tagtatgaca tttcatgaag gtgattatat
    12841 agcaaatgaa aaagatagaa cttttaaagt tgttggtata attaaaaaac ctgggtttga
    12901 acgttataat ggaattgcaa cagctatagc ctactttaat cctatgaata attacaatga
    12961 tacaattaat gtatcagtag cagtaagaaa tcctaaagat gtgtataaga taagtaataa
    13021 aataagtaaa aatatatatt caaataaaga taaagaagca gtttctgata tgataaaata
    13081 caatgaacat ttattgaggt tacaaggtgc tagtaagtat tcaaatataa atagctctat
    13141 aaagtctata attatagcag taactatttt agtaatcata tgtacaattg ctacagtgta
    13201 caattccttt agtatttcta taaatgaacg aaaaaaacag tttgggatat taaattctat
    13261 aggtgctaca tctagtcaaa ttaaaagatt agtttttata gaaggtatta taattagttt
    13321 aattggtata ccaataggac taatatctgg aacagtagct atagacctat tgtttaaaat
    13381 tataaataag tattttactg aatcggttgt tactaagatg agtttgcaga tagtgtataa
    13441 tccaataata attattgtta gtataataat agttttgctt acaatcttta tatcaattct
    13501 actacctgca atatcggctt ctaatatttc tccccttaat gtgattaaaa atactggaga
    13561 gtataaagtg ggcaaaatca aaagctcaag acttattaaa atgattttta aaactgaagg
    13621 agtacttgca tataaaaata ttaggagaaa tagaaaaaaa tttataataa ctcttttttc
    13681 tttaatggta agtataataa tatttatatc ttttagtggc tttacattac ttttactaag
    13741 aggtgaagaa attagaaatt ctcaaagaaa atatgatttg tatttaactg caaagaacac
    13801 agctaggtct gtagaggata ctataagtga actagaagat attgatggga taaaaaactt
    13861 tgaattggca acaggacaat atgtgtctat aagagtaagt gaaaataaaa taaataaatc
    13921 caaggaagat ttaatcagga aatattatca aaaatataag attggagaaa gttatgaata
    13981 tgatttcagt aataatgaat tgatttttcc gggagatttt gcagtaaaaa acaaaataaa
    14041 taacttagta cagggctctt ttaataaaga gagagcaatt gaagaaaatg gtgttatatt
    14101 agttagaaaa agtgcaattg aagaaaatgg gaagaaaggt gtagtagaac ttaccaacta
    14161 taaagtagga gatacagtaa attgtgaata tcttgatgaa aatgccttaa gtaaaaaagt
    14221 aaaggtcaag attcttgcta taactgatga agaacgtcta ggttttggtt atcaaaatat
    14281 ggggttacaa tttataactt atgatgaagt tgctaaaaat ttaaatttaa aattaagtaa
    14341 aagccttata tttattgatt caggaggaag cattaagact aagaaggaaa tagaagcttt
    14401 ggcaaataaa aatgatttta acttttatga tgaaatttca agcagtgaaa gtgaaaaaca
    14461 agatttaaag gttattaaaa tatttgtata tggttttata gtagtgatat ctttagtgag
    14521 tgttacaaat attctaaata cagtaagtac aagtataaat ctaagaaaaa gagaacttgc
    14581 agtaatacaa tctatagggg ttactcctaa aggatttagg aaaatgatat atttagaaag
    14641 ttttatatat ggtatactgt cattgttgtt tggaatacca attagtattg gtataattct
    14701 aattatgaat aaacttattt caggtgtaat tgaattttct ccgattatac cgtggacagc
    14761 catagttatc tgtatagtaa gtgtttttat aataactttt atagcagggt atataccaat
    14821 gagtaaatta aataaagaaa atataataga taata
//
LOCUS       DAMJVX010000088        14793 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.092, whole genome shotgun sequence.
ACCESSION   DAMJVX010000088 DAMJVX010000000
VERSION     DAMJVX010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14793
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.092"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            132..1199
                     /locus_tag="PC407_002317"
     CDS             132..1199
                     /locus_tag="PC407_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HDF2497760.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESVILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEESKDISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            1218..2378
                     /locus_tag="PC407_002318"
     CDS             1218..2378
                     /locus_tag="PC407_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2497761.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEETELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            2391..4487
                     /locus_tag="PC407_002319"
     CDS             2391..4487
                     /locus_tag="PC407_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDF2497762.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGFNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTKEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(4646..4990)
                     /locus_tag="PC407_002320"
     CDS             complement(4646..4990)
                     /locus_tag="PC407_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2497763.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            5282..6172
                     /locus_tag="PC407_002321"
     CDS             5282..6172
                     /locus_tag="PC407_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2497764.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            6390..7676
                     /locus_tag="PC407_002322"
     CDS             6390..7676
                     /locus_tag="PC407_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2497765.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDICEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKKFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(7765..8208)
                     /locus_tag="PC407_002323"
     CDS             complement(7765..8208)
                     /locus_tag="PC407_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HDF2497766.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(8232..9026)
                     /locus_tag="PC407_002324"
     CDS             complement(8232..9026)
                     /locus_tag="PC407_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HDF2497767.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(9592..10068)
                     /locus_tag="PC407_002325"
     CDS             complement(9592..10068)
                     /locus_tag="PC407_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2497768.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPNVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(10169..11338)
                     /locus_tag="PC407_002326"
     CDS             complement(10169..11338)
                     /locus_tag="PC407_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2497769.1"
                     /translation="MLNIRTLCHEINDWIINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKNYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            11501..12364
                     /locus_tag="PC407_002327"
     CDS             11501..12364
                     /locus_tag="PC407_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDF2497770.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDDLYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            12435..13028
                     /locus_tag="PC407_002328"
     CDS             12435..13028
                     /locus_tag="PC407_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HDF2497771.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(13279..13914)
                     /locus_tag="PC407_002329"
     CDS             complement(13279..13914)
                     /locus_tag="PC407_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2497772.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNIHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(13994..14725)
                     /locus_tag="PC407_002330"
     CDS             complement(13994..14725)
                     /locus_tag="PC407_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2497773.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLYMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQNNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
BASE COUNT         5610 a         1749 c         2380 g         5054 t
ORIGIN      
        1 tatcacatta tattttggaa aagagtaaat tttcagtaaa ttatcttgac atatatattg
       61 aagtaataat ataatttaaa gtataaaatt ttaaaaattg aattataact tcaaaaaata
      121 aggagggtaa tatgaagata acagatatca aatttgaaaa gcttagaatt aaattaaaaa
      181 aacctgtagt agtttcattt ggagtaattg aatatggaga aagtgtaatt ttaaaaattg
      241 aaactgatga agggtattgt ggatttggcg aagcagcacc tcttgcagca gttacaggtg
      301 aagttttgga taatgttctt tcagtattgc ttatgtttaa aaaagagtta ataggcaaag
      361 acccactaga tattgaaacc atacatacaa tcatggatgg aattattata ggtaatactt
      421 ctgcaaaagc agctattgac atagctttat atgatattaa aggaaaaatt atgaatgcac
      481 ctctttataa agttcttgga ggatttgata gtaaagtcca aacggatatt actataagta
      541 tagataagcc agaaaaaatg gcagtagaag ctttagaaag agtaaaagaa ggttttagga
      601 tattgaagct taaagcaggt ataaatcctg aagacgatat tgaagctgta aaattaatta
      661 gagaagccgt tggagagagt ataagaatta gaattgatgc aaatcaagga tggaacgtaa
      721 atagcagtat aaatactatc aaaaaattag aagaatttaa tgttgatgca attgaacaag
      781 cacttccaca ttgggattta gatggtactg cttacataag aaataaaagt aatacaaaaa
      841 ttatgataga tgaatcgtta cattcaccaa tagatgcaat taaggctata aaaaagaatg
      901 cagttgatac atttaatatt aagctgatga aatcaagtgg aatatatcct gctataaaaa
      961 tcaacaacat agctgaggca agtggagtaa attgtatgtt aggatgtatg ctggaaacga
     1021 gaattggtat aacagcagca gcaaatttaa tagcatctaa aaagaacata acagaagctg
     1081 accttgatag ttttatgttt tgtgaagaat caaaagatat atctggagga tttgtaatgg
     1141 atggagatat tatgaatctt gtcaataaac caggtttagg aatagaagta aacttatgat
     1201 aaatttggag gttaaatatg aatacacttt tttcaaatcc aggtagtata cttgctgtaa
     1261 tgacatcaat gattgcattg ggtttttact tacaaagata caaagccatt aaaagcttag
     1321 gaccagcact tacaataatc ataatgggaa taatactttc aaacttaaaa gtagttccag
     1381 taagtactga gttatatgga actatttcaa catatgctat cccagtttct atgactataa
     1441 tgttgatgag tgttgactta aaagaaatga caaaattatc aagagaacct ttaattgcaa
     1501 tttttgtagc tgtattaact gtaagtatca tggcattttt atttggatta gtatttgcag
     1561 aaaaaatatc agaaggttgg aaggttgctg gaatgtttgt aggtacatac actggtggaa
     1621 gtgctaatct tacagctatt ggaacaggcc ttaatgtaag tagacaaact cttgctgcag
     1681 caaatgcagc agactatgta attggagtac caacattgat atttatgttt gctctaccag
     1741 caatattaaa aaattcaaag aagtttaaaa aactttggcc atatcatgta gaagaaacag
     1801 aattagaaga ttgtcaaaat gaagaattta tggagtcaaa agaatggagt attaaagata
     1861 tagcttggat gttggctata ggttttgttg ttacagaagt ttcaacaata cttgctggat
     1921 atttcaattc aagttttagt agtgcagcaa gaatactttt agttacaaca atttcaatta
     1981 taattgctca attaaagcca gtaaagaaac taaagggaaa tttagattta ggattatttg
     2041 ttgcattatt ctttttatgt acaataggtt tttcagtaga tataaaagag tttttaggtt
     2101 caacttttac tataacactt tactgtttta gcataatttt tgcttcattt gtttttcatt
     2161 taggcataac tagactttta aagataaagt atcaatatgt aatactttct atagtaggag
     2221 caattgctga tggtcctact tcagctttag tagctgcttc tgcaaagtgg aattcgcttg
     2281 taagtgtagc agtcgtaatg ggagtaattg gtggagtatt aggaaattat gcaggtatat
     2341 cagtggcata tgcaataaag atgtctctag gattataggg gggagtttaa ttgataaaat
     2401 ttaaattata catatctgga ctatacagtg gaaatgtaat ctttgatggc gatttattaa
     2461 ttgaaaagtt gaatcctttt acaaataaaa tagaaagttt aaaaccaata agcaaagaag
     2521 aaaatacata ctatttaaat cttacaaaaa tagatttaaa gtctttattt aataactttg
     2581 atgtatatac taaaagctta gtaaacacag ataaagacac agttataaat aacttaggag
     2641 aaacgcattc taaacttaat gaatatatat gggttcaaag aaacaaaaag ttcccactag
     2701 atataattat tgtagataat aaaatagtag gttttatctg tttatccaga gaaacttgta
     2761 ctatattaat tatggatgga tatgaagagt atactgtatt aaaagaatgg gaaaaaaccc
     2821 ataaaaatga agagatatat tctataagat ttggtggaaa ttatatgatt gatatgaaag
     2881 atggtataaa actttctaca gatgtatatc tccctgattt tgtagattct accaaaaaag
     2941 caccaacaat tcttatgaga actccatatg gaaaagaaaa tgataaagaa atatattata
     3001 aatatgtaca aagaggatat gctgttgtaa tacaagatgt taggggaaga aatgaatcag
     3061 aaggtaagtg ggaacctatg attcatgaga gggaagatgg agatagtacc ataaattgga
     3121 tagtatcaca ggaatggtca agtggaatag ttggaatgct tggagcatca tatcttggtt
     3181 atgtccaatg ggcagctgca tcttcaggca ataagcatct taaagcttta gttagtatag
     3241 taacatcagg aagccctttt attgatatac caagaaaagg aggagctttt gtatctggta
     3301 tgcttgcatg ggcatttatg gtatcaagaa ataaagttga tagaagtaaa atggtaagag
     3361 atgattggga tgatgtttta aacatacgac caatagaaaa tatacctgtt gaagctcttg
     3421 gatatagaat tgaatttctg gaagaatggt taaagagagt agaaaaagat gagtattggg
     3481 atttgatgga ttggcattta caaaaagata aaataaatgt acctgcattg gttgtatctg
     3541 ggtggtatga tgataatagt atgggaacta cagaggcctt agatgttata aaggactatg
     3601 aaaaaggaaa gagaaaggct attctaggtc catggatgca taattcaaat actttaagag
     3661 atataaatgg tatttcttta ggaaatagtt cattaagata tgatttggat tataattatc
     3721 tattatggtt tgataaatac ttaaaaggta tagaaaataa catagatacg actgctccag
     3781 tagagtatta ctctgtaggt tttaataagt ggaagacaga agaaaattgg ccaataataa
     3841 ataagataga taaaagtatg tatctaataa gtgatggaaa tgctaacaca tctttaggaa
     3901 atggtagatt agtatttgat aatgatttag aagaaaagta tgatagttat atatataatc
     3961 caaaagaccc atcagtacaa ctaattgata tgtcagagaa tgaagttgga gttccaaata
     4021 actacaaaga tttagaaaaa agaagtgata tgttgtgtta tacatcagat gcttttagtg
     4081 aagaatttac tgtaacaggt gatataaagt tagagttttt tgcatcaagt tctgccaaag
     4141 atactgactg ggttataaaa attatggacg tggatttaga tggaaattca attaaattag
     4201 ctgatggtat acttagtgct agatttagaa atagttttta taagtcagag tttatggaag
     4261 aaggagagat ttataagttt acgattataa cttcaaaaat atcaaacacg tttaaagttg
     4321 gtcataaaat tagactagat ataacgtcta gtgctaaaaa ctttattttc caaaatagca
     4381 acacgaaaga gggatataat agtattgaat atatagaagc aaagaatacg atttaccatg
     4441 gaggtaaata tccatctaaa ttgatattgc caatagaaaa taagtaaatt tatatagatt
     4501 aaatagtgag gaggtgtctc aaaatgaatt ttttagttcg tgagacactt tcttttatac
     4561 gaaatcaaat atattttcat catttcaaca atgaaaaaga ggcttatctc tctgttttga
     4621 gatagcctcc tataagctaa attgattatt ttgtagctgc aacaacgctc atttcaacta
     4681 acaaatgctc tctagcaagt ctagcttcaa cacaagctct agcaggttca aatccttttt
     4741 caacccaagc atcccaaaca gagttcatag cttcaaagtc tttcatatct cttaaataaa
     4801 tagtaacaga tagcaaatgt tgcttatctg aaccatattt gtttaataaa tcttctattt
     4861 tagctaaaac ttcagttgtt tgttcaatta ctcctccttc agcatgagtt tgtccacata
     4921 aataaactgt attgttatga actacagctc tactcattct tccagttcct tcatatctct
     4981 ttatttccat attttaaata cctccatagt taagaaatct attaaaaatc taaataataa
     5041 attaaaaaaa gtctatacta tataaaatta tattatatat tctgataata atccataaaa
     5101 acatataact ttttagctat aattattatt gttattaatt gaaaagactt attagttact
     5161 tgacatagtt tacactttta attatagtat aaattggaat atttagataa ttattattta
     5221 atgtataagc taagttttta attaatatgg ctttatacat ttatatagaa aagaggtacg
     5281 tatgagtagc acaatcaaat caaatcgaat aaaaggtata atgtttataa tagcatcagc
     5341 gcttggcttt gcaatgatgt ctgcattcgt aaagctagct ggagatttac caagttttca
     5401 aaaagtgttc tttagaaatc tggtatcagc tataatagct ttttggctta tcataaaaca
     5461 taaaggaagt ctaactggta aaaaagaaaa tttaaaagta cttttatata ggtcaatatt
     5521 tggaactcta ggagttatat ttaactacta tgcaattgac aagttgatgc tttcagatgc
     5581 taatatgcta aataaaatga gtcccttcct tgtagtaata ttttgtgcaa ttgctctaaa
     5641 agaaaaaata aatgtaaagc agataggagc tattataatt gcatttatag gagctttatt
     5701 cataataaaa ccaacattta gtgtagaagt aattccatat cttgggggaa ttgcaggagc
     5761 tgtatttgca gcaatggcat atacttgttt aagagttctt ggagatagag aagattacta
     5821 tacaattgta tttttctttt cagttttttc attagttact gtagggccaa ttgcttttgc
     5881 agtatatgaa ccaatgaccc ttatgcaact tatatatcta ttactaggtg gagtatttgc
     5941 aagtctaggt caatttggaa taactttagc atataaatat gcacctgcta aggaaatctc
     6001 tatatttgac tatagtaata taatcttttc agcaatttta agtatattct tattcaatgt
     6061 ctatccagat atgttaagtg ttgttggata tttaatagta ttttcggcag cattttatat
     6121 gtttttatac aataagaagt tagataaatt agacaaagaa aagaagaagt aaactagata
     6181 aaagtgtttt tataatttgt attgaatatt taagtctttg tattaaaatt aaagaaaatt
     6241 taattaaaaa tgtttggtat attaacttat aacagcagtt aaaattgaaa aattataact
     6301 gctgtttctt ttattaaaaa tatccaaatg tctgtaaaaa tagactataa tagagtagta
     6361 taaataatat aaaggggata aattaaagta tggaaaaagt gaaagttgct gtgattggag
     6421 caggtaatag aggaacgtat gcatatgctc catatattta tgaaaattct gatatatgtg
     6481 aaatagttgc tgtagcagaa cctaaaaaag gaagacgaga actatttaca caaaaatata
     6541 acctagatag taaaaatgta tttgaaactt tagaagactt ttttaaacat gacaagatgg
     6601 cagatgcagt aataatagct acaaatgatg atagacatta tgatgtagca aaactagctc
     6661 ttgaaaaagg ttatcatgta ctattagaaa agccaatgtc taatagttta gatggtttgg
     6721 tacatataga tgacttgtgt gataagtata aagataagat ttttatgatt tgccatgtac
     6781 tgagatattc tccattttat aataaattaa aagaaattgt agaaagcaaa aaattaggag
     6841 agctagttag tatacaatat aatgaaaata taggatattg gcattttgca catagcttta
     6901 ctagagggaa ttggagaaat agcaatgaaa ccagcccttt aattttagca aagagttgtc
     6961 atgacatgga tattttgtta tatttagtag gaagtagatg caaaaaaata tctgcatttg
     7021 gaagcttaaa gcatcttaat aaccaaaatg ctagtggaga gatggcacaa aattgtcttc
     7081 agtgtttagt tgaaaaaaaa tgtccatatt cagcaaagag aatttattta gaaaaagata
     7141 gaagtataaa tagagctgtt cacataaatc caactgaaga gaatctatta aatatactta
     7201 agacaagtcc atatggaaga tgtgtttaca gatgtgataa taatgttgta gacaatatgg
     7261 taaatatatt acagtttgaa aatggagtga cggctacttt taatttatgt gcatttacta
     7321 aagaaaatgg aagaactata aagttaatgt ttagtcatgg tgaagttggt ggggatttaa
     7381 ataaaaatga gataagaatt aaaaaatttg gaaaaaacga agaaatagtt atgaatccat
     7441 caaatcaaca gaatatggta gaggaatatg atagaaatct tatagctgaa tttattaaat
     7501 tagtttcaaa taaagaacta gaaaagggta gagtagcagc aaaagaagct atacaaagtc
     7561 atgtaatggc atttgcagca gaatattcta gggtcagtga tgaagtagtg tatatagaag
     7621 aattttttga tagtgctaag caaatgacta aggagataga agaaactata ttttaattaa
     7681 taaaaattta ttaaataaaa taaagataga ttaaataata gaaatacgaa aatatgtgtt
     7741 ctattaagga atctatcttt tttattaagg ttttttctta tccctatcaa aaaaatccat
     7801 acattctgat aaaagataag aaagtaaagc gaataagatt tctgtactta aaggaatcgt
     7861 tactgaatcc ataaaggtat caattatgtt catagctaga aaagttagta taaaatcaat
     7921 tataaattcg caaagtttat atattgagta tggtaatttg tttacatatc tgaatatatc
     7981 taaaatagta gtgcatagaa aatcaattgg agtggttata caaaaatata ttaaaaagaa
     8041 aattaataca gctccaagag atttatattc aaatcctaag aatgataaga agccaattcc
     8101 tataaccgtg aaaatacaaa aagttatacc aaaaaaaaca gcaaaaaata aaaatacaga
     8161 aataaattcg cctaaaggaa tccttctttt attacttatc ttaatcaact ccttaattta
     8221 tgatatgaaa tttatataaa taataacatt cttaaaaatt cataatagtc ctctgtttca
     8281 aatagtactg tttgagaatc tatttttttc atatttggat aaaaagaggc cttaaaagct
     8341 ttataatgtt ctctaaattt tatttctatc ttaaatttat ctggcaattt tattaagtgc
     8401 ctagataaat ctccagataa agcttttttg acaccttttt caattttttt tagtgcaagg
     8461 tttggatgga ttgaaattga accatttcca attccttttg acacaggaac agtgactata
     8521 tttggattta gtttttttgc actctcacaa agcatttcat ctccacttaa aaatgctaca
     8581 ggaacattat aatatgcagc aatataagca tttatagtaa attcagatgc tatttcatca
     8641 tttattttaa aatagtcata atcaatatcc atagtatgag aaagtggatt accattacaa
     8701 gtagctccag agtgatagcc ggtgaatata catgcatcaa agctttcatc aattccagcc
     8761 atcataacta gtgggtcttt ggtccatcct ctaagtattt tgacgttttc aggtaaaata
     8821 tttggattaa tatttctacc actgtcatgt gcatctttaa caagtatttc atgtacacca
     8881 gattgagtag ctccatcaca tgctgctgtt atctctttac tcatttgtgt tctatagtgt
     8941 tgtgaaaatt cacattcaag ttttgtttca tcccaattta caataccaca agtaccttct
     9001 atatcagcac ttaaatacaa tttcataata tataccccct tatgtttatt attgatgata
     9061 ataacagata acagaaaata cataatcaat aaagggcata aaaatattat gtcttactat
     9121 taattattat taaatctgta actgaaaaat aactaaattg gtattaaaaa atataacact
     9181 atgtgtcatt tatttatata gttttttata tctgatgttt atataaaatt gtaacatata
     9241 agtggttaca tttactagtt taacatagtt aaaatagtta ggtattattt ttaagttaaa
     9301 ttgattatta taaaatcgta gaataaaaga aaaatttaat ttattaaaat gcatgaaatg
     9361 ttttttcctc aacataatgc taaattaaac tcaaaaagaa ataatagaaa aatcttatgt
     9421 agcttagaaa gaatgtgaat gatttaaggg aaaaatagaa atttattata taatattagg
     9481 ctgtattatg taatacagcc taatattata tgtatatgtg acataaaatc ttggtaagtg
     9541 taaataaaat aaatgtggaa aacaaattaa tgaaatcaag aaaaaagtaa tttaaaattc
     9601 aatttcatct tcccaattaa attctcctga tttttctaca ctcttaggcc agtgactaca
     9661 ccatttagga acggacggat tttcaaccct atcaacgctt ggagtatatg gctttaagtc
     9721 tataacagga ctattatcat tagcatcaat atatggtatt tttattatac cattttcatg
     9781 gtcgatattg ataatttgta cagcagtcaa tgctattgga ttgggtctta taggtgacct
     9841 tgttgcaaat attcctataa cgtttggtcc acttttataa ggttttgggg tttctaaaat
     9901 acttcttagt tcaggattat caaacccatc agcccaccaa aatacattta aatgactgaa
     9961 atcgtcgagt tcatttaatg caggtacata ctttttgtct aatttaataa agacctcttc
    10021 gccttgaata catattttac ctataggatt tattaatatt tctttcataa tattacctcc
    10081 aactatttaa tttatcattt gtaattttag ggtataacat gacatcatgt gagggtcaat
    10141 ataaaataat aaataaaaat attaatagtt agaatgataa tacatttttt acatggagca
    10201 tgacaccagt aataaggcaa tcttcgtcaa tatcaaaagt agaggtatgc aaaggggaag
    10261 taatgttttt ttcacgattt gcacaaccaa ggtggtaaaa agctccttca cagtgtctta
    10321 gaaaatacga aaagtcttca gccccaagag tgggcgagtc tttaaggata atattttctt
    10381 ctccaaacaa ttctacagca ttttgcttga cgtaatctac tagatggttt gaattgataa
    10441 gagctggata accttcctca atttcaacag aaatacttcc tccaaatgtt ttacaagtaa
    10501 aatcgcaaat ttctctaatt cgtttttttg aaaactctct agtttcagga gtcaaggttc
    10561 tcaaagtacc ccttataacc actttgtcac aaactatatt ttccttgatt ccaccttcaa
    10621 tctttcctag agaaataacc acagaatcag ttggatttgt gtttcgacta acgattgtct
    10681 gtagagctgt aataatatga gcagatataa caatagcatc aataccttga tgtggataag
    10741 ctccatgaca ttttgaacca tgtacagtta attgaagtgt atcggtggaa gcatttagag
    10801 tgttgtattt taattcaatt agatttttgt tgatattggg atttacatgg agtccaaata
    10861 tatattttac atttgggttt tcaagacatc catcttctat cataagttga gccccaccaa
    10921 tagtttcctc ggcaggttga aaaaagaatt ttacatttac atttaagtaa ttctttaatt
    10981 taaatagtat attacaagta cccagtaaaa tagcagtgtg tgcatcatga ccacatgcat
    11041 gcattttacc aatattaata gatttataag aataattaag ttcttctgtt ataggtaagg
    11101 catctatatc agctcttata gcaactgttt caaagtttgg actcacgtta atgtatgcag
    11161 ttatccctgt atgattctta tattcaatat aatttatacc tatttcattt aagtatttaa
    11221 taattttttt cttagtttga aattcttcta atccaagttc tggagtctta tgtaaatctc
    11281 ttcttatatt tataatccaa tcatttatct catgacaaag agttcttata ttaagcataa
    11341 aacacatcct aactatatta aaaatttatt tttcttcatt aattatatta ttttatgtaa
    11401 atcattatta tgtatcaagt acaaataaaa tatcttataa ttatattaga gatatttagt
    11461 atggagaaga atatatcatt ttataagaag aggtgtttaa atgttaggaa taattgcgcc
    11521 atctgggcca ttaagaaata caagtttgga ggaaataaag tttaatttag aaagttatgg
    11581 ttacgaagtt aagttttctg aaagttgtag tttaaattat aaaggttatt tagctggtaa
    11641 tgatgatatt agagcaaggg atatagaaga tatgttttta gataaagatg tagatataat
    11701 tatgtgctta agaggaggtt atggaacaac aagaatattg gataaaataa attatgatat
    11761 aataaagcaa aatccaaaac catttatagg tttttctgat ataacaggtt taaatttagc
    11821 tttttataaa aattgtggac ttttaccata tcatggaata atggcagcaa atgtaggtaa
    11881 atgggataaa tttacctata aatcattggt aaatgcttta gaatttaaag atgacttata
    11941 tttagaaaat ccaaaagaag aaaagattta tactgtatgt gaaggtaaag cagagggaat
    12001 aataatggga ggaaatttat ccttaataat tgccacaatg ggaacaaaat atgaaataga
    12061 tgcaaaagat aaaattttgt ttattgagga aattggagaa cctcaatata aactggatag
    12121 aatgttaaca caactatatt cttcaggaaa acttgaggaa tgtaatggta taatttttgg
    12181 agattttaag gattgtattg aagaaaatga tttgatggaa ttactaatag aatttgcaaa
    12241 taaggtcaat aaaccttcta tatataatct tcagtcagga cattgtatcc caatgataac
    12301 tattcctttg ggcagaatgt gcgaattaga tgctacagaa aaaatcgtta aattaaaaaa
    12361 atagtgtgta tataaatata atagaaatat ggtataatct tatacaagta aaaatttagc
    12421 taggaggatg aaaaatgtct gatgatagaa taatagactt taacgaacta aagaacaaag
    12481 ttaaagattc tgatgtagat aaatttgagc aatacatata taacttatat ttttctgtta
    12541 tggatggaaa gatgtccatg gctgagtttt caagaaaaat attcgattat atgagagata
    12601 acaacatatc tcaagaaaaa tttatgaaaa tacaaaagca atttatggag agatatggta
    12661 tggacactga agaagtagaa aaacaactta gaaactttgg aatcgaccct tctacagcag
    12721 gttttatgag taataatact tccagcaaag tatctacaga agatttagag tcatttaaga
    12781 agagtgcagg attttatgag aaatatggag aaaaaatcca accaaagagt tgtataacaa
    12841 catttataaa aaatgactta aatgatatta atgtaataat cgaccaagaa aagataatgc
    12901 tttgtagtga tagaaaaata aatcttatgg attcagaact aaatgagttt ttattagaat
    12961 ataagaatat gtttaataaa aaaataaagg tagtaatgtg cgaaactacg aataagtatg
    13021 attattaaaa aatgaattta aatactgaag tagtatcaat taattgtact acttaaaaat
    13081 aaaaatcgca atttattatt ccttataaat ttgtataagg aatatctaaa ctgcgatttt
    13141 ttatattgtg aagattagtc ataatagata gtgttttaaa ttgtaatctt ctcactaaaa
    13201 ctgtgttttt ctattagaaa ttttattaaa aataaatatc tgataattgt attatatggg
    13261 atgtaaagta ttagaggctt aacgaatctc ttttaataca ttaactccat tttgaatcat
    13321 actgttgaaa aatactaagt ttaaaatctc agcagggtga atgttaggaa tatcataagt
    13381 atctactaaa ttcataggta caactatatt tttttcaata ttatgttgat taaaatatga
    13441 ttttagagtt attgcaaatt gatatatgca tatatctgtg caatcgccaa ctataattat
    13501 attatctata ttatcaaaat ccaaattttc taatgcaaaa aatccattag tagaattttt
    13561 aggaagaatt tgtaggtttt ctatcgactt aagttcatca accaattcac tttctacatc
    13621 attttccaaa cagtgaacag gataactcaa aagttctatt gatttaggag tatgagaatc
    13681 agtaaatgct attactctat ttaatttgtt tgatattttt tttgtaaaca tttcaattgg
    13741 ttttattaaa gactctactc taggagagta taatgcacct tgtcttgcaa agccattatt
    13801 aacatcaatt ataaataaag cagttttact caaatcataa ttttctattt tttctattgg
    13861 tagattatct aaattactct ttaaagcttc aagttcattt aataagttat ccatagttat
    13921 acctccaaga taaatttaat aaaattgtac atattaatta tataaaagtc aaggagtaca
    13981 aactattgta taattagctg tattgttttg agtgtaaaag atttgaagca atatctggat
    14041 agttagtaaa aataccatct acattgagtt ttagacagtg tagtatatca tcctcttcat
    14101 ttacagtata tacattgaca ccaagtagat ttttgtgaca aagttcaatt agagattcat
    14161 ttactaaaac tagaggtgga tgaaggtaac tattacccag aaatttagca aaattgacta
    14221 cattgtttat aggtaaataa cacaataatc ctgtttttac actagggtct aatttatgaa
    14281 agttttttaa gctattgctg ttgaaactag atataagaac tctatttttc atattatact
    14341 ttaaaataag ctctaaaacg tctttttcta tattgggata gtttattttg tcagttttta
    14401 gttctatatt taaaataagg tctgtaggtg aaattagaat taatacttct tctaaagttg
    14461 gaattttaca ttctttattg ttttgatatc cttcaaaaga tgagtttaga gtttttaatt
    14521 ttcttaaagt aaagtctttt acaaaaccat gtccattaaa agttctatca accttttcat
    14581 catgaataac tacaattttt ccatccttag ttctgtggac atcaagttct attccatata
    14641 tatccatata caaacatttt ttaaaagcta gaatagtatt ttctggatac tttccactaa
    14701 accccctatg agcataaata ttcatttata tggttccctc ctataagtat aacttatctt
    14761 ataaaaattt aattaatata atatatcata ttc
//
LOCUS       DAMJVX010000089        14739 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMJVX010000089 DAMJVX010000000
VERSION     DAMJVX010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14739
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.093"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            327..1175
                     /locus_tag="PC407_002331"
     CDS             327..1175
                     /locus_tag="PC407_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDF2497774.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGTEEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            1254..3176
                     /locus_tag="PC407_002332"
     CDS             1254..3176
                     /locus_tag="PC407_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2497775.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDIHDSSVIAAYLEGCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            3258..4121
                     /gene="aroE"
                     /locus_tag="PC407_002333"
     CDS             3258..4121
                     /gene="aroE"
                     /locus_tag="PC407_002333"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2497776.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGIEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNAKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIESSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            4321..5538
                     /locus_tag="PC407_002334"
     CDS             4321..5538
                     /locus_tag="PC407_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2497777.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAILIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLIVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            5774..7639
                     /locus_tag="PC407_002335"
     CDS             5774..7639
                     /locus_tag="PC407_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HDF2497778.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIEYNKIEFEKEINDILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(7817..9181)
                     /locus_tag="PC407_002336"
     CDS             complement(7817..9181)
                     /locus_tag="PC407_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2497779.1"
                     /translation="MSQLLIKNAYLITMNADREVYETGSILIENNIIKNVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLIRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHNPGAAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(9328..10671)
                     /locus_tag="PC407_002337"
     CDS             complement(9328..10671)
                     /locus_tag="PC407_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2497780.1"
                     /translation="MVPNTNNVQPDGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(10744..10845)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            11175..11828
                     /locus_tag="PC407_002338"
     CDS             11175..11828
                     /locus_tag="PC407_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2497781.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNSFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDEIRGAIFDVSQAIDLTKLMGIIIK"
     gene            11969..13240
                     /gene="brnQ"
                     /locus_tag="PC407_002339"
     CDS             11969..13240
                     /gene="brnQ"
                     /locus_tag="PC407_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2497782.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAIFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            13442..14629
                     /locus_tag="PC407_002340"
     CDS             13442..14629
                     /locus_tag="PC407_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HDF2497783.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMTKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
BASE COUNT         5478 a         1783 c         2544 g         4934 t
ORIGIN      
        1 attatagagt aagaataaaa gcttttaaaa aaatttatcg ttttttgtct gaattataag
       61 ataaatatat ttatacaacc taatagaaaa atacaagaat tatcttataa ataaaatcta
      121 taaacaaaat ctatgaatta ataggaaatt atcaatttgt tattagtaaa gtctcgtgat
      181 acaataaatt tataaaatct agataaatag aaatcaaaaa atacttttat ttttaaggta
      241 aaataatact actagtagga taaataaaaa tataaaatca aagatactat taattataaa
      301 gaaaataaag ggggcaaatt taagctatga ataatcaaaa aaatcttaat attccaatta
      361 caattagttc ttatacattg ggaacagaag tttcattccg tgatagagtt cgtattgcaa
      421 aagaagctgg atttgatggt attggattgc gtgctgaaaa ttatattgat gcaaaaaatg
      481 caggtgttac agatgaagaa atgattgcaa ttcttaatga atatgatatg aaagttacag
      541 aagtagaata tataactcaa tggggaacag aagaagatag aactgaagct caaagagaaa
      601 aagagcaaaa tgtttatcat atggctcact tatttaatgt taaacatata aattgtggat
      661 tattagagat gataccagaa gaacaaatta taactgcatt gggagaatta tgtgacagag
      721 caggtgaact tattataggt cttgagttta tgccatatag tggtgtacca gatttggcta
      781 ctgcatggcg tgttgtgaaa ggatgtaatc gtgaaaatgc aatgcttata ttagatactt
      841 ggcactgggc tagagcaaag cagacagcag aggaccttgc acctgttcca gcagataaga
      901 tagtatcaat tcaagtgtgt gatgttttag aaactcctta tgaaaaactt agggatgaat
      961 cactacatga ccgtttggct ccaggtgaag gatatggaaa cacagaggaa tttgtgaaaa
     1021 taataaaaga acacaatata tcaccaagag tcataggggc agaagtaata tctgattctc
     1081 taatatctaa agggttatcc actgctgctg aaactgtatt taatgcaact aaaaaagttt
     1141 tggataaagc atggtctgaa gtatctccaa aataaactta tataatataa aaatatacag
     1201 agtattataa tataaaactt ttaaatataa aacttgaaag gaaggaaaac cttatgaaga
     1261 ttagttctat gtttacacct ataagaattg gttcaatgac agttccaaat cgttttgtag
     1321 ttccccctat gggaaacaat tttgcaaaca cagacggtac tttaagtgaa acttctaagg
     1381 cttattatct tgagcgtgct ttaggtggtt ttggattaat aactatagaa tctagtgttg
     1441 tagacaaaaa agcaaaagga ggtcctcgta agccttgttt atatgatgat agcacaatag
     1501 atagttttaa aaatgtaata gatgcttgtc atgatgcagg ttcgaaggta tctatacaat
     1561 tacaacatgc tggttctgaa ggaaatgaaa aagtagcagg acatccatta aaagctgctt
     1621 ctgcaatacc tgcatcaaat ggtcgtaata caccactagc aataacaaca gaagaaatat
     1681 atgaacttat agaatcttat ggagatgctg cattacgtgc acaaaaagct ggtgcagatg
     1741 ctgttgaagt tcactgcgct catggatatc tggttagtag ttttatatca caaagaacaa
     1801 ataagcgtgt agatgagttt ggtggatgtt ttgaaaatag aatgagatta cctcgtctta
     1861 tcattgaaaa tatacgtaaa aaagtaggaa attctctagc aatattatgt cgtataaaca
     1921 gcactgatga tgttccagga ggaattgata tacatgatag ttcagtaata gcagcatatc
     1981 tagaaggttg tggaatagat ggacttcatg tttcacgtag tatacatata catgatgaat
     2041 atatgtgggc tcctacgact ttacatgctg gattcagtgc agaattagtt actgaaataa
     2101 aaaaagctgt tagtatacca gtaataacag ttggacgtta tacagagcca cagtttgctg
     2161 agttaatggt cagacaagga agatgtgatt tagtagcatt cggtagacaa agcttggctg
     2221 acccagaaat gcctaataaa gctaaaaatg gaaaactaga cgaaatgata ccatgtattg
     2281 catgcttaca aggatgtgta ccaaatatgt tccaaggaaa acctatagcc tgtcttgcaa
     2341 atcctatatt aggacatgaa gcagagctta agccagctga aattagcaaa gaagttcttg
     2401 ttgttggtgg aggtgttgga ggtatgcttg cagcatgggt atgtgctaaa agaggacata
     2461 atgtaacttt agttgaaaaa tctgaagttc ttggtgggca aatgcgttta gctgcatatc
     2521 ctccagggaa aggagatatt actaacttag ttagaagtta tatttcaaaa tgtaatcaat
     2581 atggtgtaaa aatatgtact aatacagaag ctacagtaga acttataaag gagaaatcac
     2641 ctgatgtagt tattatagct actggagcaa ctccacttgt acttcctata ccaggaatca
     2701 atgattcagg attaattcat gctgtagacc ttcttgatgg taaaaaatct tgtgggaaaa
     2761 aagtacttgt tgttggaggt ggaatggttg gatgtgaagt ggcagccttc cttggagaac
     2821 aagaacatga ggttactgtt attgagctta gagaagaagt tggtgcagat gtaatttcag
     2881 aacaccgtaa attccttatg aatgattttg aaaattataa tatacatacc attactggtg
     2941 caaaagtttc taaattttat gatgatggtg tcgactattt acttacagat ggcacagagc
     3001 atgatttaaa aggttttgat tcagtagtac ttgctatggg ttctcgtaat tacgacccat
     3061 taagtgaagt tataaaagaa gttgtaaaag aaacttatat tgttggtgat gcagtaaaag
     3121 ctcgtagggc attagatgca acaaaagaag cttttgaagt agcattaaat atataaataa
     3181 taaataaatt tttctttgta aataactgaa taggttattt gcaaagaaaa tgataatatt
     3241 gggaggaaat tatagttatg gaaatttcag gtagaacagg attatttgca ttaataggta
     3301 caccagttgg tcactctaaa tcaccagtga tgtataatta tagttttaag aaattagatt
     3361 tagattatag atatttagcc tttgatatca cagtagataa ggtaaaagag gcacttttag
     3421 caataaaaac ttttaatata aaaggagcca atgttactat gccatgtaaa tctgctgtta
     3481 cagaatatat ggatgaactt tctccagcag ctcgtataat tggagcatgt aatacaattg
     3541 taaatgacaa tggaaagtta gttggtcata taacagatgg agtaggctat gttcgtaatt
     3601 taaaagaaaa tgggatagaa gttaaaggta aaaagattac aattatgggt gctggtggtg
     3661 cagcaactgc tatacaagta caatgtgctt tagatggagc aagagaaata tctatcttta
     3721 atgctaagga tgatttttat aaaagagcag agcagacagt tgagaatata aaaaaagatg
     3781 taccagaatg tgttgttaat ctatatgatt tagaagatac aaacaaactt tatgaggaga
     3841 tagaatcaag tgatatattg acaaatgcaa ctcttattgg tatgaagcct tatgacaatg
     3901 aaacaaatat aaaagatact agtgttttaa gaaaagactt agtagtaaca gatgtcgttt
     3961 ataatcctaa gaaaacaaaa atgattgaag atgctgaagc taatggatgt aaggctattg
     4021 gtggattagg aatgttatta taccaaggag cagaagcttt taatctatat actggattag
     4081 aaatgcctgt agaagaagtt aatgaattat gctttaaata gataagagtg atatcttaaa
     4141 caatgaaaaa acatacgtat ttcaatattt ttaataataa attacaaatt gtcatagtgg
     4201 gttttgcatc atagtccagt taaatttgaa gctttaactg actatacttt aaatccatta
     4261 tgaggatgag tttattgtac acaaaaatgt taataaatta acaagaatgg gagaaaaatt
     4321 atgaacaata aaaaatatta tcctactgcc atagcgttgt atttttcata ttttttactt
     4381 ggtattggaa tttcaatttt aggacaatac aaaccagaat tttcatcaat gtggggggct
     4441 aaaacactat cagatggaac attggatgta agtattgttt tagctgttat tgctgcacta
     4501 ggattaggga gacttatatc ttatccattt gcaggaccta tttcagataa gtatggaaga
     4561 aaagtaagtg gtcttatagg aaactttcta catgcaattt tctttgttgg aattgtattt
     4621 tcaccaaatt tttatatagc atatgtattt gcaataattg gaggggcagc aaattcattc
     4681 ttagatacct gtgtaactcc ttcttgtatg gagatatttg ctagtttagg aacaatagca
     4741 aatatgttta ctaaatttac aatagcttta gcacaatttt tgcttccttt tataattgga
     4801 tttgttgcag caaattcaat atcatttaaa gttattttta tagtaacagc gattcttatt
     4861 gtagtagatg ccattttgat agctgtgtta ccatttccac cagctaataa tgttattgat
     4921 aacaaaggca aaacagttaa atcagagaag atgaaattta caccaacaag tatagctctt
     4981 gtttgtattg gatttacatg tacatctaca tttgtattat ggttaaattg taatcaggaa
     5041 ttgggtaaat tgtatgggat ggctgaccct acaaaaattc aatcctttta ctcaatggga
     5101 gtaatatgtg cagtattaat aacttcttta cttattaaaa aatatattaa accaataaga
     5161 atattagtta tatatcctat aatagcttta cttatgctat taattgtata ttttgttcaa
     5221 actccaacta tttgtatgtt agggggattt gtaataggtt attcagcagc aggaggtgtt
     5281 cttcagttaa caacgtcaac tgcaaatgaa atgtttccaa ctaataaagg aaaaataaca
     5341 tctatagtta tgattgcatc tagtatagca aactatgtta ttttaaatat agcaggtatt
     5401 attacaaaat cagggggagt taatggacca aaatatgttg tattgtttaa tgtggcaata
     5461 acatttgttg gaattttact tgcattgttt gtaaacatga gatatgaaaa agaaaaagtg
     5521 tatgattatg atgtctaata ttagtataat atatgcatag agaagtctaa atctaggata
     5581 gatttttatt aaaagtaatt aaaattaata aggatattat ttacagttat aagatagggt
     5641 atttacataa ttaataataa ggatattact agaatttcaa tttctagtaa tatttttttg
     5701 cgtataaatg taaacttgac gaaataagta gtataatcaa tataaattac atcatgtaaa
     5761 ggaggataat ttggtggaga atgtttttaa gcgattgcag gaatttaatg gttatgatgg
     5821 atacaaagaa tcatttgaaa tgaattacct atgtatatat gaaagtatac cgttaagaga
     5881 acaagtagaa cttgcaaata atctagtaga tgaaatttta aatatgtata aaagtgaatc
     5941 taatgaaatt tatttattgg aggatagtaa ttctaagtct ttaatttgtt atttcgaaat
     6001 ttttatgaaa aagataaata ctttggtaaa agaaatgatt attgatgaga aatggttata
     6061 taaattaact aaagaattaa tatataagag taaaaaagtt gagtatgtaa aactagggtt
     6121 agttttaagt gagaaatatc taaatgtaga aaacttaaga gaagttgtag atacattttc
     6181 taaatctgga gaatacgtat tttatctgag taatactatt aaaaaacttg aattttacaa
     6241 tacatattta tttaatttat ctaaaaaggc tacaggttca ataaaagtat ttgctattgt
     6301 taatatggaa aatttagatt ctaaaataaa ttcctatcta attgaagatg gttataagga
     6361 tacaaagtac gaacgattat tgatgaacta tattatttca attgtagatt tgaatgaata
     6421 tcttgaaaaa cgagatttag ataaagaaaa gataaataat ttagcacgtc ttatatgcaa
     6481 ttacttgcta agtgtagaat ttaaatatat aggtaataaa ttagaattag tgaatcgatt
     6541 tttacctact gtagtaaatt atgggactaa ctttgaaagt ttatattcaa ttttcctaat
     6601 agctataaac gtcttaaaag atgaaaatat agagtacaat aagattgaat ttgaaaaaga
     6661 aataaatgat atcttgctca gtgaaaaatg gaaaaatata tattttgaag cattaagaga
     6721 tgctagtgga aaaacagaag atattataaa aatgtcagaa atttatgatg taaacttatc
     6781 ttttgatgac ttgttgccat atctgaatag agatattaga gattttgagg tatattggca
     6841 tatatctaaa aagggaacga caagttctag attaaaatta ttaaattttt ttgaagagac
     6901 gtttaaaata gatgatttaa ttggaaaaat gaaagatata gaaaaagata aattgactca
     6961 agaatattat gatgatatgt tattttttat tgtgcttaaa ggaagtaaat cactatatcc
     7021 tgaaggaaag aatatatctt taaaaggaat atttggaaat ataaatgaag taagaaaaga
     7081 atccataaat atcttaaaaa gatacagaga gaaactatct cttgaagaat taaaaattgt
     7141 taaagaagcg tatgaaaaag aaaaaaatgt tatattgaag gatgaattaa gaagagtatt
     7201 atatgaaagt aataatttaa aaaaagaatt tgtaaatatt gaaaaaataa aagtagatga
     7261 acatggtaag gatatctatt tgacttcaat agctgttgct ggttcaaggt ttagaaatag
     7321 agaatatctt gaaaaagaat tggagaagag taagatatac tatttaacta gagagaaaga
     7381 taatctatat gatgaaaaag ctattaaaat agttggtgaa actggatatg taataggcta
     7441 tgtcccaaga aaagaaaact atattttgag caatttatta gatggaggaa aactgctgta
     7501 ttgtagagta actgaatata atttatatga agattgtatc tacgcaaatg tgtacttgag
     7561 ttataaagat gtaattgaaa cagttgaaaa ctctttaaag atggttttag ataagagtag
     7621 aataaaactt ataaactaag acgtttatat attgcttaga tgatgtaatc ctaactgatt
     7681 ggttattttt tctggaatta tggtgtattt tatgagcaat gttaatgaat ataaaataaa
     7741 aaaacaattg ttttgtatat atgatgtgta taaatatatt tatatattca tatttacaaa
     7801 acaattgaaa tttatgttat tttatgattt taaatttaga tggaatttta acttctgctt
     7861 tatcttttat tttagaagct tgttttttaa ctttatttaa taattcagat tcatctacaa
     7921 ctaatacttt tctttccttc atcaaccatt taccatcaca aatagtagaa tctacatttt
     7981 cactagtcat agaataaact atatttccaa ttggattatg cattggtaaa caatgaattg
     8041 tatctggatt tagaataatc atatcagctt tttttcctac ttcaagacta ccaatttctt
     8101 tttctagtaa agcacatttt gctccattta tagtagccat ttctaagact tcttcagctg
     8161 gtacgacagt tgggtttaaa gttctacctt tatgtattag tgatgtaaga tacatatccc
     8221 tcataaggtc cattctatta tttgaaggag ctccatcagt accaattgaa acaggtattc
     8281 ctttttctaa catttcagga attgaggcaa atcctagaac tactttcata gctgcacctg
     8341 gattatgaga aacttttaca tcatgtaatc taaataaatc aatttcttta tgagtaagcc
     8401 aaactgtgtg agcagctaaa agatttggtc ctaatacacc tagtttatca agatattcaa
     8461 ctgttccaac tccgttgttt tgctttacaa atgaaatttc atcagctatt tcagcaatat
     8521 gcatatgtat acctgtattg tatttatcag ctaactcttt agtcctaata agcaattctt
     8581 cagttgtatt aaaaatagtt cttaatgcaa accatatttt aatcctctca ttagatgtgt
     8641 tattaaactt atggaataag tctaattgag tgttgatttc ctcatcagta gttttttgcc
     8701 atgcttcagg aagaccatct cctgtatcca taactgattt agctaaacag gctctaagtc
     8761 cagttttatc aacagcttca accatagcat ctacatattg accaccagat tctaaaaatg
     8821 tagttacccc agattttatc aattcaacac aacatgcagt agatgaaatc aatgaatctt
     8881 catagttgaa gctactttcg tatggccaga ttctctctct aagccaagtt aaaagtacaa
     8941 catcatctgc aagaccacgt cctaattgtt gggataagtg aacgtgtgta ttgactaacc
     9001 ctggcattag tatttttcct ttagcatcat atatctcagc atctttatct acaatattaa
     9061 tatcaaactt accaacattt tttataatat tattttcaat aagtatactt ccagtttcat
     9121 atacctctct atctgcattc atagtaatta gatatgcatt ttttataagt aattgtgaca
     9181 taagtacaac ctcttttcta tatattttta tatctatgta ttatgataat aaattttcta
     9241 tacaaagtta atagcaaaat caatttatac ataaattgat tttgctattt ataaatttaa
     9301 aatattgacc tagaactatt taaaatatta acttagaact aagtttacaa ctgcaagtaa
     9361 acctataatc caaagtgtaa tactgatttt atcagtttct ttagctacca catgcattat
     9421 tatatatgaa ataaatccaa agcataaacc aatacttata ctataagtta aaggcatcaa
     9481 taaaactgta acaaaagcag gtacagctat tttgctatca gcaaaatcaa tctgttttat
     9541 gctttcaaac atgaatacac caaccattac aagtgcagga gctgcagcgt aagtaggaac
     9601 tataccaagc aatggtgtaa atataagtgt agctaagaat aatgctgctg ttactattga
     9661 agcaagtcct gttcttgcac ctgctgcgat accagaagca gattctccaa atgttgtaac
     9721 agtactagtt cctaagaatg agcctattaa agtagaagaa acatctgcat ataacattct
     9781 tcctaaatct tttatttcac cattctcatc ttcaagaccc atttgtttac aacaagtaat
     9841 aagaaatgct aatgaatcaa acatgtctat aaacatgaaa gagaatatag gtccaattaa
     9901 tgagaatttc aatgcaccta caatatctaa tttaaaagca actggtgcta tactaggagg
     9961 tgttgagatt attgctgttg gcaactccac aaatccaaat ataacagata ttattgttgc
    10021 aactactata cttatcaata ttccaccttt aactttttta tattctagta ttgccattaa
    10081 gataagagca aaaagagcta ttaaaactgt tggtgtaaaa tctgcaagtg aaactatagt
    10141 atctggatta gctattataa cacccatatt ttttaaacca acaaaagcaa ggaaaagacc
    10201 tatacctgct gaagatgcta ctgccaattc tctaggaatt gcgtctgcaa ttgtctttcg
    10261 aagacctgtt actgcgagtg taaagaagac acatccagat agaaaaacta ccccaagacc
    10321 tgtttgccat gggattcctg cacctataac caaagagtat gtgaaaaatg catttaatcc
    10381 cataccagga gctagagcaa atggtgcatt tcctaaaatc gcaaatataa gtgtaccaat
    10441 ggctgtagct agtatggtaa ctgttataag ggcggcttta tccattccag cttcagataa
    10501 tattgctgga tttacgaaaa ctatgtatgc cattgttaag aatgtagtaa ctccacctat
    10561 tacttctgtt ttaaggctag agccacgttc gctaaattta aacattttgt ctaaaaagct
    10621 taatgatgaa ttatttccat ctggttgtac attgttagtg tttggtacca tttttacctc
    10681 caaaaaactt taagatataa acaaaaaaat atatattcat atagatagaa tagaacatat
    10741 attcaaagtt gatattctat tcatagaaaa cccatttaag gtgagtttgt agaaactatt
    10801 agaccgtatt actaatatta tatgaacatt ttctatttgt ttttctgttg tattatatcg
    10861 taatattgta ttatttacaa taaatataat aaaattattc gtttttttgt cgattttata
    10921 aaaaattaag gttatttatt ggcaagaatt actgttttca ataattgaat atgtataagt
    10981 ataatgtcaa ttattctaac ttgttttata ctattatata gaagtctatt ataaatgagt
    11041 ttgtgatata attataaaag tactgaatac acggatattt cgtaaaaact ttttaatata
    11101 tcaaatatgt atattaagtt aatatattaa aaaaaataat atcaaaaata atataattta
    11161 tagtggaggg gaaaatgact aaaattatta aagtgcaaaa acctaaattt acaggagaat
    11221 tagaaattat tgaaaatctt gaagatatac ttgaagatat atttaatgat gaaaaaatat
    11281 ttaataaaat tatagaaaat gaagttataa gtgatttaga ttctgtaaga gttagctttg
    11341 aatcttgtat atttagaaat gtatcctttg aaaattgcaa tttaaaaaag attgatttac
    11401 ttgatgtggt atttgaaaac tgcaatttat caaattcatt ttttgatgga ggaagtatat
    11461 acagagttga atttaaaaat agtaaattga taggagttag atttgatgat tgtatactta
    11521 aaaacgtttt gttcaaggat gttttaggta agtattctaa cttttcattc tctaaattta
    11581 agatagtttc tatcttagat tcaaattttg aaaattctgt atttcaagaa gtaaaaggtg
    11641 acaatctagt atttgagtat acaaacttaa aaaaagcaaa ctttaatgga actaaacttt
    11701 ctggcataga ttttaccact aataatattg agggaattga gattggtata gatgagatta
    11761 gaggagcaat ttttgatgtg tcacaagcaa ttgatttaac gaaattgatg ggaattatca
    11821 taaaatgaaa aaaatattca taaaataaat tgggaaaacg ttaacaatgt gtgaattttt
    11881 accagattga catttttctg aaaataaagt aagattatct taatattcaa aatattttca
    11941 aagggggtaa tttagtgtga gtaagcagat gagagatatt tttatagttg gttttgcttt
    12001 attttctatg ttctttggtg caggaaatct tatgtttcct ccatttttag ggatggaatc
    12061 cggcaaagac tggttaattc ctcttattgg atttgttgtt gcagatgctg gtatagcaat
    12121 gcttgttatt atagcaacag caagatgcaa gggaagcatg gatgatgtac ttaggagagc
    12181 tggtaaaggg ttagcaagag taatgagtgt agctgctcta gcatgtttag cacttcttgt
    12241 aataccaaga acttgtgcaa caacatatga aatgggtatt atgcctatat ttggagacca
    12301 tggaccagta gctaaagcta tattttcaat aatcttcttt gggttaactt tagcatttac
    12361 tattaggtct tctaaggtta tagacataat aggtaagtat ttaactccag cgttattatt
    12421 agtattattt gttttaatag taaagggtat aatttctcca gcaggaccta tgagtcctga
    12481 gcatatgata gataaaaatc tatttggtga aggtatatca caaggttatc aaacaatgga
    12541 cgctctaggt gcagcatcta tggcaactat aatgttgatg tctataatag ctaaaggata
    12601 tacatcagat aaagaccaaa taggaatgac tattaaagct ggttttgtag cctgtgtatt
    12661 tctagcagta gtatatggtg gtcttactta tttaggtgca acagtttcaa ctttatatga
    12721 tactagtata cctcaagcat ctttattagt tgaaataact agtatattgt tatatggtca
    12781 agtaggaaag gtaattttag gtgtaattgt ggcacttgcc tgtctgacta cttctacagg
    12841 tttaacagca tcaatatcaa cttattttga aggaataagt aaggcgagtt ataaacaact
    12901 ggttataggt atatgtgtag ctagtatgct tatatcaaat ttaggtgttg attctataat
    12961 agctatatca gttccaatcc tacaaactat atatcctgtt ttattagcaa tagttgttat
    13021 ggagttggct ggtaaacata taaagaatga taatgctttt aaaggtgcag catatgttac
    13081 attagtgatt agtctattat cagctataaa tggaatgaca ggagctgttc catttataca
    13141 aagcttgcca ctcgcaggtt tgggctttaa ctgggtaata ccagctatat tgggtggaat
    13201 agttggtaac tttataaaaa gcaacaaaca agtagcataa taatttgtaa ttaatttaat
    13261 tttataatat attaattttt agtactatgg gtaggtttaa tctaaaccta atctacccat
    13321 agtacttttt agattggatt ggaatagcat gtatataatt ttttataata taatttttct
    13381 ttgaagtatg ataaaattag taatagtgat aagaaagtac aaatttagga ggttttaatt
    13441 aatgcttaga aaaataacag catttatgct atccatatgt ttaattattg gacttacagg
    13501 ttgtagttta cttgtaggca agaaagataa agagaaaatt atagatgatt ttaaagttgc
    13561 agtggtaact caaccattat ctgaaaataa agtacaatac aatatggtgg aagagatgac
    13621 aaaagaatat gaggaagaga ataaaattga taaagacaaa gatggtcaaa ctaaagttaa
    13681 acaaactata aaacatgttg tattgccaga gaattttact tctaatatag atagtgctat
    13741 taataagata gtaaagttgg ctgacgataa agaagttcag gctattgtag tttcaactga
    13801 ccaagcaggg cttttacctg ctttacaaaa ggtaaaggaa aagcgtccag aaataataac
    13861 tatttctgcc cctatgggag atgataaaaa ccaattaagc caatttgttg atgtgaatct
    13921 aggggttagt gcagaggaaa gaggaaaagt attagctgaa cgttcaaaag aaatgggagc
    13981 aaaagctttt attcattatg cctctacaga tgatttgaaa gatgttaata tagccaaaag
    14041 attagaaatg attaaagaga cttgtaaaaa tataggattg ccatttgtac aagttaatac
    14101 tccaaatata aatactgaag aagacaaaaa caaagttaag cagtttttaa atgaagatat
    14161 tgaaaaacaa gttaaaaaat atggaaaaga tataaatgta tttggtgtaa atgagtatat
    14221 ggatgaagtt atattgacta aggctttaga attaaagtat atagttgcag agcaaagcaa
    14281 tccatcacca atacaaacgt atcctagtgt tatgggtcta aaaatatctg aaaaagatgc
    14341 tcaaaattat gataaaataa acgacatgat aagtgaaaag gctaaggctt ttggtatgtc
    14401 aaatagactt ggtggatatc ctatgccaat ggatgctttt ctaccatcag tagcaattta
    14461 tcttgctact gaaatggtga aacaagattt aacacaagaa gatgtatgtg acccagatta
    14521 tttagaggcg tttacagaat taagatttgg aatcggttca gaatttactc ctttaacaga
    14581 ggtactttat aattatcaat cagtaatttt aagccaatta atttattaat attaaggaag
    14641 tgtctcaaaa tgaacttttt agtttatgag gcactatttt tctgttaaat caaatatatt
    14701 tttatctttt taacaattaa aaagaagctt atccctatg
//
LOCUS       DAMJVX010000090        14541 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMJVX010000090 DAMJVX010000000
VERSION     DAMJVX010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14541
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.094"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(5..1033)
                     /locus_tag="PC407_002341"
     CDS             complement(5..1033)
                     /locus_tag="PC407_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2497784.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDINKKMDTIDKLKDI
                     EEGYFYLLEDVYLDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVVNKI"
     gene            complement(1081..1941)
                     /locus_tag="PC407_002342"
     CDS             complement(1081..1941)
                     /locus_tag="PC407_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF2497785.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKSI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2220..2429
                     /locus_tag="PC407_002343"
     CDS             2220..2429
                     /locus_tag="PC407_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2497786.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2429..2695
                     /locus_tag="PC407_002344"
     CDS             2429..2695
                     /locus_tag="PC407_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2497787.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2724..3296
                     /locus_tag="PC407_002345"
     CDS             2724..3296
                     /locus_tag="PC407_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2497788.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3530..4720)
                     /locus_tag="PC407_002346"
     CDS             complement(3530..4720)
                     /locus_tag="PC407_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2497789.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENVENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSAGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(4980..5411)
                     /gene="dut"
                     /locus_tag="PC407_002347"
     CDS             complement(4980..5411)
                     /gene="dut"
                     /locus_tag="PC407_002347"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDF2497790.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5524..7752
                     /locus_tag="PC407_002348"
     CDS             5524..7752
                     /locus_tag="PC407_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HDF2497791.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEYEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(7863..8348)
                     /locus_tag="PC407_002349"
     CDS             complement(7863..8348)
                     /locus_tag="PC407_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2497792.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            8600..9823
                     /locus_tag="PC407_002350"
     CDS             8600..9823
                     /locus_tag="PC407_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2497793.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTKESEPEKPIAVSVQKAVGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNANNKFKAGMSAQVNLSIEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            9827..12961
                     /locus_tag="PC407_002351"
     CDS             9827..12961
                     /locus_tag="PC407_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDF2497794.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            12995..14293
                     /locus_tag="PC407_002352"
     CDS             12995..14293
                     /locus_tag="PC407_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDF2497795.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINNVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNQDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
BASE COUNT         5364 a         2125 c         2134 g         4918 t
ORIGIN      
        1 tttcttatat cttgttaaca acatttaagt tattacattt atttatatat attttttcat
       61 tatctaagtc ttcatcaaac atttttatta tattttttat ctcatacttg tactcatctt
      121 cattcattct ataaagcatt aaatcttcta atacatcttt catgtcgagg tacacatctt
      181 ctaataaata aaaatagcct tcctctatat ctttcaattt atctatagta tccatttttt
      241 tatttatatc tttcataatt tctaaatttt gtatatttac atctatgatt gttaataagt
      301 acattctcac agtttttatt atttcaacag aaaaatctac aatattataa tttccttctt
      361 ttttatataa tgactcctta aatttgttaa aacaattatc taaattagaa tttatatcgt
      421 taaatggtgg tcttattatg tcacgtatta ttaaaagatt ttgtatatct acattatttg
      481 tgattgcctc atccaaaata aataaattat atgcatcatt taatgggtca tgggcatctc
      541 cactaaactc taagttaaca aattcaagta aatttttcaa tgatatatag ttcatacatc
      601 tcataccaca atttatatat ttttctttta tatctacaaa taaatctcta atattattta
      661 caaaccttgg atggttattt tttttagcac ttctcatatc tgtgttattg aaacatgtca
      721 aatccaaatt accaaaagta tatattcctt taatatcaga aaatatgcca agccattttt
      781 taaattttct catgacctct tcataacttg gtgcactctt caattcttca gaagatatat
      841 gtgttaattc ttgtatatga ggatataact cctcattact tactggtctt attaaagatt
      901 taaatttatc aatatttttt gttttttcat catacataac tgctccgatt gcaattatat
      961 cagaattaaa tactgctctt tttgatttag aatttggcat attcatttca aaatctatat
     1021 atactctttt caatataaac accctttcca attgtttata gtgattcttt ttaactattt
     1081 ttattcaaaa ttttctaaga aagaatgttt ctccccatct ttttgccaac caagtactga
     1141 atcaagattt acattttctg ctgccttaaa tatagatttt tctacatttt taaaatttga
     1201 ttctaagctc tttattaagt cttttatctc atatctttta ttacctgttt ttttagcagc
     1261 aaccatacat gcacaattta atggccatat accactattt tgaataaatc ttataatatg
     1321 ttcttctctt atataataaa gtggtcttat aatttttata ccttcaaaat tagtagaatt
     1381 aagtttagga agcattgttt taaaatttcc agcacacaat aagttaagca ttgttgtctc
     1441 tattacatca tcataatgat gacctaatgc aagtttgtta caccctaatt cttctgcctt
     1501 tgaatataat gctcctctcc tcattcttgc acacatatag catggataat cttttgctat
     1561 ttcatctgct atctcaaata ttcttgaatc aaataaatgt attggtatat ttagatactc
     1621 acaattatct ataagtaatt gtcttatatt tgcatggtat ccagggtcca tagcaataaa
     1681 ttcaacatca aaattaactt gtccatgttt ttttaactct tgaaacattt tagccataag
     1741 aatactgtct tttccaccag aaatcgctac agctatctta tccccttctt caaccaattt
     1801 ataatctctt attgacttca taaacttaga ccataaattt tttctatatt tctttattat
     1861 acttttttct atatctctta ctggaagtct ttcctcaaaa ggaactataa tttcacagcc
     1921 ttttcctgat agttcactca tctaatcacc tcgtatattt atgtatattt atgttaataa
     1981 tattttacaa aaaagtctac cttcatccac aaaatactat aatttttact ttaattttac
     2041 taaataattt atagtttaca ataacacata aacttataat aacataaata acctctttct
     2101 tttattatct tttttatttt tctcatttaa tattttatta ttagtaacga tttttaaatg
     2161 attgaatatt atacactaga tagagttacc tatctattta caacaaggag gttttcatct
     2221 tggaaaataa gactagagat atcaaaaaaa attgtggtcc atctttggct agaccataca
     2281 ttaataatca aattttcaca gaaatgtact gcctttctga tgcattaaaa tttggaacta
     2341 tatttccaga actaaactta ctagagtctg aaatgtacaa caaagaatta tatgctaagc
     2401 caaaaaaata tagaggaggt agaagataat gaaagactca tctagagctg aacttatgag
     2461 aaaagttcag gaaacatctt ttgcatgtgt tgatatgaac ttatatttag acaatcaccc
     2521 tgatgataaa aatgctatca acacttataa ttcattatgt aatcaatttg cgcaagctag
     2581 atatgcttac gaaaataaat acggtccttt aactaacttt gggtatgctc caagtagatg
     2641 cccttggcaa tgggctgacc aaccttggcc ttgggacaga gaatttaatt attagacatc
     2701 aagtattgga ggattgaaaa ttcatgtgga tatatcagaa aactatacaa cacccagtta
     2761 atataaaaac ttgtgaccct agaatggcta aatttcttat aactcaattt ggtgggccaa
     2821 atggagaact tgctgcatct ttaagatatt taagccaaag atatacaatg cctactggta
     2881 atatgcgtgc acttttaaca gatattggta cagaagagct agctcacgtt gagcttatat
     2941 gtactatggt ttatcaatta acttctgatg caagcccaga agagttaaaa gctgcaggtc
     3001 ttggttcaaa ctatgctcaa aatggatatg gaatttatcc aacagattca aatggtgttc
     3061 catttgatgt aagacctata gcagttatgt caaatcccgt aaccgactta catgaggata
     3121 tggcagctga acaaaaagca cttgcaactt attatcaact tataaaccta acagatgacg
     3181 ttgatgttat agatgtatta aaattcttag gtcaaagaga aataattcac tatcaaagat
     3241 ttggtgaagc tttaatggat gcttacgagt tagaagaatc tcaaaaaatg ttctaaaatt
     3301 tttatttgta taactattat tatttgataa tttataattt actgttgtgt taaaatttat
     3361 aaaaaatata aatataattt attttctata aacagagaaa ataaataaaa agcaggtatc
     3421 aatcaaatag taacatgtat tatactatac attactatca atattgtacc tgctttcata
     3481 ttataagcaa taaagaaaaa tctatttatt aggttttata ataaaacaat tataaaactc
     3541 ttcttgcatt tacatatcta cttgaatagt agctactatt tatattagat attgttactt
     3601 tttttgatga acctgaagat gcatgtatca tatctccatt tcctatgtat attccaacat
     3661 gacttacaga accattattt gaaccttgtg tatcaaagaa tactaaatct ccacttctaa
     3721 ggtctcctct atttacatat gttccatact tactttggtc tctagatact cttggtatac
     3781 taactcctgc agattttttc ataacgtact gtgtgaatcc agaacagtca aaactatttg
     3841 gaccttctgc tccccataca tatggttttc caagtagtgt ctttgcaaaa cttattacac
     3901 tatcagctcc ttgacttgga gaattagtcg aataagatgg aattgaagta tcaacagaac
     3961 ctttttcagc aaggtattga cttgacacat atccagtttg attgccataa cttacttttg
     4021 tccatcctcc actttcagaa atacttccaa cctcagtacc ataacttaat gtagctattt
     4081 tagaatgact tgtacctggt cctgttctaa cgttcaatga ctttgctgtt acaacctttg
     4141 taccctttac aaggtcattt gaactgtttt caacattttc atttgtagag tcatttacac
     4201 tagttcctaa atatttactt gaaacataac ctactctacc attagaactt atttttgacc
     4261 atccatttga ctcagatatg tatccgactt cagtcccata tcctagtgtt gatattttag
     4321 aactacttgt acttggtcct gttctaaaat ttaatccttt tgctgttaca acttttgtcg
     4381 acttaactga agtatcacta ctttcattgc tacttccaag gctatttatt gctacataat
     4441 ttccatgcac ataacctgtg tttccattat atttaacttt tacccaagaa ccttcttttc
     4501 ctacgtactc aactttatca cctttgtaaa gtttccccat tgattcatgg tttgtacctg
     4561 gtccttttct aaaatttacc gcattccctg ttattgttct gctttctata ctcacactct
     4621 ctacttcaga ctcattagca tatgctaaat tccccatagt aggcattatt atcgcagttg
     4681 ccattatact ggctgctatt ctcttttgat taaaattcat aactttccac tcatctcctc
     4741 ttttctacta tctttatttc acttcaaaat ataaaagaaa taattgacac tattaaaata
     4801 tcaattattt cttttatatt aatattatta caaatttgta actatttgtc tatagtaata
     4861 tttgcttacc ataaattacc atgtataaat tttaatcttc aatagcctgt aatttagtaa
     4921 tatagccatc ttttaccttt tttaaaaatg gctatatttt ttaacttttt tgtaactttt
     4981 taaaatccag ttgagccaaa cccacctttt cctctttcgg agtcacttaa ttcttttact
     5041 tcttctatgt taatatcata gataggtttt actatcattt gagcaatttt catatgcttt
     5101 tcaaccttaa aatcattttt cccatgattt attaatataa ttttaagctc tcctctgtat
     5161 ccttcgtcaa tagttcctgg tgtatttaat acagtaacag aatgctttaa ggcaagccca
     5221 cttctaggtc ttacctgcgc ttctgtcata gtaggaagct ctatacatat cccagttttg
     5281 ataagtttag tctctccagg aggtattaca acctcttcta tagaatacaa atccatacca
     5341 gcatcacctt tgtgagcaaa attaggtatt atagcatcat catttaattt ttttactttt
     5401 aaattgtaca tcttatatct cctttcaact ttctataaat attataactt aaatttcgac
     5461 ctcttgaagt tttttatttt tttgtggtaa tataattatt agtgttttaa aggaggattt
     5521 tttatgaaac ttgtacttgc tgagaaacca tctgtggcaa aaactatagc atcattttta
     5581 ggtgcaaaga ccagacaaga tggttacttt gaaggaaatg attatataat tacttatgca
     5641 gttggacact tagtatcatt atatgatatg aaggattatg ataaagataa atactctggc
     5701 tcttggaaaa tgaacaattt cccatttgtt cctgaggata agtttaaatt taagattgat
     5761 agttctaaaa ctaaacaatt caacactgta aaaaagcttt tgaatagaaa agatgtagaa
     5821 tatgtaataa atgccactga caatgaccga gaaggagaac ttatagcatt tttaatattt
     5881 ttactggcaa aaaataaaaa accagtaaaa agaatcttgg taaatgaatg gacaccagaa
     5941 gatattacaa gaggtataaa aaacctaaaa gatgaatatg aaatgagaaa tcttcaagct
     6001 gctggatata ctaggcttat tactgactgg ttgattggaa taaatttcac ttctgttgct
     6061 acattgaaat atggaaatgg gaaactatta aacataggta gggtaattct tccaacagtt
     6121 aaattagtct atgatagaga tatggaaata ctaaactttg ttcctaaaac ttattatgaa
     6181 atagaaggac attttaaagc tgaagctggt gaatataaag gtaaatatgt taagggtaaa
     6241 gaaagtaaat ttgatacact tgaagatgca aataaaatta tagcttcaat tacatctgaa
     6301 actggtaaaa tactagataa aaaagttact atgtcaaagg aatatgcacc aaaacttttt
     6361 agtttgacat cacttcaagg atatataact tctaagtatt ccaattttac atctgataag
     6421 gttttaaatg tctgtcaatc tctctatgag ggtaatggta agggtggata cataacttac
     6481 cctagaactg attccatttt cttagaagaa agtttggctt caaaagcttc tcaaacttta
     6541 gataaattaa aggttggtct tgaatatgaa aataaaatta aattttctaa aactaaaaga
     6601 gtttttgatt cttctaaagt agactcccat agtgctataa ttccaactta tataattcca
     6661 aaaagtttat ctaaggatga acaattagtt tatgatgcca taaaagatag atttgtagca
     6721 aactttatgc ctcctgctga atatgagaat acagaaatta agactgaagt agataattgt
     6781 actttcttaa caaaaggtaa agtgcttaaa tcaaaaggat accttgaggt atataataag
     6841 gaagaaaaaa acgatttact tccacttgta aataaagatg atgttgtaga tgtgttagaa
     6901 ataaaaccat taactaagca aactactcca cctaaaccat atactgagga caccctttta
     6961 aaggctatga aaaattgtgg taaaaatgtg cctgaagaag atacaacagt gctatctgga
     7021 tattcgattg gaacatcagc cacacgtgct gatgtgttaa agaagataag tcaagtgggc
     7081 tatgtaaaga aaaaagggaa atcttattct ataacagacc ttgggaaaaa tttagttgaa
     7141 atttttcctg ttaaagactt atttgatgta gattatactg gaaaacttga aaaaagttta
     7201 agcgatattc aaaaaggaaa gtatacaaga aaagaatatc ttactaatat cattagtttt
     7261 atatatcaaa atgtcaatat tataaaacaa gatgcttcaa aaaatataaa cacagaagca
     7321 tatactttcg attcaaaaac taaaaaattt actaaaacaa aagaaaaaaa acagaaaaaa
     7381 gatgtaacta atactgaaaa agaagttgct atatcaaaag attctaataa caaaaaagaa
     7441 gttaaaaatg gtgatgaaaa tcaatctctt ggcaaatgcc ccatttgtca aggtgatgta
     7501 ctagaatttg acaaaggctt tgcttgtaaa aatcataaag aatgtaaatt tgttatttgg
     7561 aaaaatgata agtatttaag tctttataaa aagaaagtta acaaaacaat ggtcaaaaat
     7621 attttaaaaa aaggtgaaac taaagtaaaa tctttaactg ctaaaaacgg taataaattt
     7681 gatgctacat taaaatatga taaaaatcca gataccggct atttcaattg gaaaatagag
     7741 tttgacaact aaatattcat aagaaaagaa atagcagtag tataataatc tatattatat
     7801 tactgctatt tcttttctta tgaagtttta acttttttaa tagtgacttt tacaatcaca
     7861 ctctaaccta atttctttac tatttctgat aactttaatg tatgttcatc taataaagtt
     7921 agttcctctt cagaaagatt agataatttt tttactagag attctttgag aatagctttt
     7981 actgcatcaa ataatttaaa tgctttatct gttaattcaa ccctaagaat ccttctatct
     8041 tttgggtcag gaaatttatt gactaaacca tgttccacta atctgtcaat aatcggagtc
     8101 atatttgatt ttaaaattcc taaattagaa gctatttgag aaattgatga tgaatttgat
     8161 gtcataagat aaagaattac cttagcatgt gatggagcca ttggacattc ttcaaaataa
     8221 gctttcatta tatcagagtt attttgaaac ttttttaaca aatcgctttc tttaaatata
     8281 ttattttcaa gcacaaataa aaaatctaaa aaagagtccg ctattttaag tatattgttg
     8341 ttatccatat ttgtaacatc ccttcaatag ataatataat aaaacattgt tgtttaatat
     8401 agtataaatt ataaactatt aataaattta aaacaagaaa aagttattga ctcataataa
     8461 ttataaattt ataatcaaat tagagaatta taaattgcca ataaagggaa gttttttaat
     8521 tagtctaaaa ctaaacttaa attataagac actttaaatt ctacaacaag ctagtgtgca
     8581 atatataata ggaggagata tgaaaagata tttaatagtt ttcttaatgt gtagttcact
     8641 gtttttagtt ggatgtggaa aaacaaaaga atctgaacct gagaaaccaa tagcagtttc
     8701 tgttcaaaaa gcagtaggtg gagaaattga aaacacaaat tctttctcag gaactacaaa
     8761 agttaaagat gaaacagctg ttactgcaca gactgttggc acagttcaag aagtgtatgt
     8821 aaaacttggg caaaatgtaa gaaaaggaga tgaattattg agtataagca gtccagagct
     8881 agaaaatagt gtaaagcaaa gtaaagcctc actagattta gcaaaagcaa gttattcaag
     8941 tgccactggt ggttcattag aggcacaagt aaatcaggca aaaacagctc ttgacaatgc
     9001 aaagattcaa tatgaggagt ctcaaagaaa ttatgacaat aataagattc tttatgaaca
     9061 ggaagtcatt agtttagacc aatttaaaaa aatagagttt tctttagagc aaacaaaaca
     9121 acagttagat tctgctcaaa gagcatacga tacagcaact tcaaaaagta tacctcaagc
     9181 taaagcactt gctaaaaaac agcttgacca agctcaagta tcatataatt tagcaatgag
     9241 taatttagac aaattaacat taacatctcc agtagatggt acaataacag ctaaaaactt
     9301 tgattcaaag gaaatgataa cacaatcaca accagctttt attatttcta atcctaatat
     9361 actagaagtt gatttaaatg tagcagaatc agatataggt aaatttaaga aagatggaaa
     9421 tgtagatgtt ataattgaag accaaaggat tttaggaaaa atagattatg taccttcagt
     9481 agttgaccct cagacatctt tatatcctgt taaagtactt gtaaataatg caaacaacaa
     9541 atttaaggca ggtatgtctg cacaagtaaa cttaagtatt gaaaaagaaa atggtgctgt
     9601 tacagtgcct aaaaaagcaa tatttgaaga aaatggtaaa aaatatgtat acatagcaac
     9661 aaaagacaat atagcaaaga aacacttggt tcaaactgga attgtaacag aggataaaat
     9721 agaaattaaa agtggtgtaa gtgataaaga tacagtgatt attggaggaa taagtcttat
     9781 ttctgacgga actaagatat ttcctgtaga aaaggagaag tagactatga atttaacaca
     9841 gacttccgtt aaacgtcctc tcacaatcat aatggtcttt ttagtattaa tagtttttgg
     9901 tggtattgga tataaaaaaa tgtcaataaa tctaatgcct gatatagaaa taccagttgt
     9961 aatggtcatg actacttgga caggtgctgg tcctcaagac gttgatgaac aagttagtca
    10021 aaaagttgat gaaagtttat ctgccgtttc aaatgtaaaa tctactatat caagttcaca
    10081 agaaagtgtt tctatggtag ttgctcagtt tgaatttgga actaatttgg atgaaataat
    10141 gaatgatgtt aggtctaaag ttgatgcatt acagaccagc ctcccagatg atgcagcaaa
    10201 accaacagtt ttaaagctgg atatgaatgc acaggctata ggtcaacttg tcatatctgg
    10261 aggaaatgaa aatagttcac aagccttaag aaaatacgct gaagatgtaa ttcagcctaa
    10321 aatagaatcc atagatggtg ttacttcagc agaccttaaa ggtggagaaa aagcacaggt
    10381 taatgtaata gctgacccag ctgttctttc aaattatgga gttagtttat ccactataaa
    10441 aggtgtctta tcctctagta ataaaacatt cccttatggt tcaattactc aaggtgagga
    10501 taaaatagtt ttaagagcaa ttgataaatt agaatcccta gatgatataa aacagattca
    10561 aattcctgta aaaggaggaa atacagttag attagataat ctttgtagtg tagattatgg
    10621 atatgcagac aaagatagta tttatagata caatggaaaa gacagtttgg taatagatat
    10681 ttctaaacaa caagatgcca acactgtaaa agttatgcag agtgcataca aatacgtaga
    10741 tgaattgaat aaggaaaatc ctcaatttaa aattaaaatt gctaatgata caagttcata
    10801 cataagtgaa tcaataaaca gtgtaatgag caacttgctt ataagtgctg ttatcgcatt
    10861 tattgtaata tttgctttcc taaaaagtgt aagagcttca ctagttgttg cagttgcaat
    10921 accaatttct ataataggtg ctatagcaat tctctacttt actggtgaaa gtttaaatct
    10981 tattactgca agttcattgg taatttcagt tggtatggtt gttgataatg ctacagttgt
    11041 aattgaaaat atatttaaat ataggaaaag tggcagattg agtcttgatg attgtgctat
    11101 tgagggaaca agaactgtaa caaatgcaat acttgcatca acattgacca cgatagcaat
    11161 attcttacct atcatgttta cagaaggtat agctaaaata acttttgggg cattaggtaa
    11221 aacactaata gcttcactag cattctcctt tataggtgca attacactcg ttcctagtgt
    11281 atttgcaaaa ttaagcagag gaaaaaatag tcaaaagatg caagaaaaag atagtcctat
    11341 atttgataag gtaagtgaag catataaaaa attattaagt gtatgtttaa gacatcaacg
    11401 cttagttgta atatcaagtt tacttatttt tgtagcttcc ttatttggaa tgggatttat
    11461 aggtatggac tttatgcctg cagcagacaa gggtgaatta tctatttcta tagaacttcc
    11521 aaaaggtctt agcctagaat ccaatgatta ctatgtatct atgactgaga aaaaagtctc
    11581 agatattcca gaaattaaaa ctttaataac aactttgaca tcagattcaa ataatagtaa
    11641 agcaagtata gcaattgaat tagaatctca aaaaaataga aaaagaacta ctgatgaaat
    11701 agaaaaagag ataattgata gagtggctac tgtaccagat tgtaaaataa atgtaagtca
    11761 aaacagtagt gttatgggtg gagctggagg agcagacttc acattagaaa tgaaaggtcc
    11821 tgatttagat gtattaaaaa tactgggaaa agaagctgaa aaagaattta gctctataaa
    11881 aggatttaga aatgttgaaa cttctcttac agatacaaca caagaagctc aatttactat
    11941 agataaacat aaggctcaag aatatggtgt aaatactgct gaaattgcag gcatgttacg
    12001 tacagcaata aatggtgact ccgtaaccac tgctactatt gatgattaca aagtggatgt
    12061 aaacctaaaa tttaaagcag atactattga tagcttagaa gatataaagc aactaaaagt
    12121 aaactctgca actggaaaag aagttcctct tggtgctttt tcagatataa aaatggcaga
    12181 tggtctacaa tctatatcta ggactgatgg agatttttct gtaaatataa ctgctacact
    12241 tgacaattta gatgctggta ctgctacaaa actagcacta gaaaaaacga ataaaatggt
    12301 aattcctaaa gattactcta taggtgttgg tggtgaaaca gaaatgatga atgaatctat
    12361 ggcaagtctt ggattttcaa tggtcatagc tatagcatta gtttatatgg taatggtagc
    12421 tcagtttgaa tcatttagta aaccatttat aatcatgttc tgtgttccat ttgcattcgt
    12481 tggagttgtg ctttcgcttc tagttagtgg tcaaagttta agtactgtcg gtatgctagg
    12541 tataatctta cttatcggta ttgttgttaa taatggtata gttcttattg actacataga
    12601 gcaacttaga aaaacaaatc gtaacaatga tttaattggt ttggtttcaa ttggttgtgc
    12661 agcaagatta agaccagtct taatgactac agcaacaaca attttaggta tgttgccaac
    12721 agcactatct cttggtgaag gtggagaaac tatgcaacca ctagccatag ttataatggg
    12781 tggtctaact gtatctacac ttataacttt ggtgcttata cctactatat acttaatatt
    12841 tgataaatgg gaaaataagt tttcagacaa aatgcctaaa atatttggta agataacaga
    12901 taaaatagga agtataaaac gtaaaataat aaagaaacca aaattgaaag ctaaagttta
    12961 aatagaatgg agggtgattg tattgaataa aaaaatgagc aaaatagttg caattggctg
    13021 tggtgttggt ataataactg ccaatttaat cccagtttac gcagataata atacttcttc
    13081 tataaacaac gttgataatt ctattttaag tgactcaaat aaaagaacat ctgaaaagga
    13141 aattttgact gctagagaag ctgttagtgc tgcacttaaa aatagtgaaa aattaaaaat
    13201 gaaatcagaa gaaataaaaa tgttaaaaga aaagttagaa gtacaagatg agtttgatag
    13261 ttttactgga agtgacaact cgtttccata cgaccaaatt ggacttctta aaaatcaaag
    13321 tgaacaggct aaaggcttta tggaggacca aatagcaaat gatattacta ataaatttaa
    13381 cgatttagtt tcaagagaaa atgagctaga taagataaaa aacaatcttg aaataaaaac
    13441 taaagaaatt aaagatatga aattaaaaaa agatttaggg ttagtaacat ctttagaaac
    13501 tgaatcagct gaacttgaac ttcaaacact acaaaatact caaaaagcta aacttcaaga
    13561 actaaaaaac aatcaagact attttaagtt attgactaat atagatttaa ataattatca
    13621 attggataaa gaatatagat ttgaatcttt tagagtaggt ggctcagttg attcatatat
    13681 ggaaggtaag gttaatgaat atttaaaata cgaccaattg attttagaac gtacagaaga
    13741 aagctttaat gataaagatg agaacaaagc agatttacca gatagaccta atcttacaag
    13801 accagttgct cctacaaaac cagagcaagg taacctaact gatgatgaat acaaggttct
    13861 tatggataag tatgaaaaag actacaagag atatgaaaaa gaagttgaag catacaatct
    13921 tgaaagacag acttatgctg ctggccttac tacttatgca aattatcttc aacaaaaatt
    13981 taataccgaa aatggtctag taacattaga agacagtaaa aaagctctta aaaaagggtt
    14041 aattgatagc tatgctcaat tacttgcttt agaagataca attcagatta ctaaaaaaca
    14101 acttgattta tcagaaaaac aattaaaaaa tactaaatta agatatgatt taggattaat
    14161 tactctaact gattataaaa aacaagtggt aagtaatcaa gatgctaaaa atagctatga
    14221 cactcttatt gtgaattata actcactaaa aaatggtata gaaaaacctt ggatacttaa
    14281 tactggaaaa taattctggt aaatagaaag attactttaa catacgtttg attaaaaaat
    14341 agttatgcac actcgtggct ttagtcatgg aagattcaga aggcaaataa tagaaataga
    14401 ttagtaaaaa tatatttact tataaaactg tatactttta tagaggctat ctcaaaattg
    14461 gatatgtccc tgtcaagtag acagacttaa aaaaggctat ctttatgcgg aatgagttcg
    14521 atattcaatc gaactcattc c
//
LOCUS       DAMJVX010000091        14044 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMJVX010000091 DAMJVX010000000
VERSION     DAMJVX010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14044
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.095"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            282..512
                     /locus_tag="PC407_002353"
     CDS             282..512
                     /locus_tag="PC407_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497796.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSIIGSIFYFCKDLLNHKIKYVLIRD
                     IFVNRITYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            569..1219
                     /locus_tag="PC407_002354"
     CDS             569..1219
                     /locus_tag="PC407_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2497797.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRMYFNRKNRIVTK"
     gene            1433..2104
                     /locus_tag="PC407_002355"
     CDS             1433..2104
                     /locus_tag="PC407_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2497798.1"
                     /translation="MAKILVVEDEKRMQSIIVEYMQKGGYTCITANDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            2101..3231
                     /locus_tag="PC407_002356"
     CDS             2101..3231
                     /locus_tag="PC407_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2497799.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDAISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLIINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRIIEQDEISKIWSKFYRSVQSQ
                     RKGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3636..4481
                     /locus_tag="PC407_002357"
     CDS             3636..4481
                     /locus_tag="PC407_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2497800.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            4481..5509
                     /locus_tag="PC407_002358"
     CDS             4481..5509
                     /locus_tag="PC407_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2497801.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGVKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            5512..6525
                     /locus_tag="PC407_002359"
     CDS             5512..6525
                     /locus_tag="PC407_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2497802.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTML
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGAPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILVVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(7007..8257)
                     /gene="cme"
                     /locus_tag="PC407_002360"
     CDS             complement(7007..8257)
                     /gene="cme"
                     /locus_tag="PC407_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HDF2497803.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHNIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLVMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIVAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(8343..9089)
                     /locus_tag="PC407_002361"
     CDS             complement(8343..9089)
                     /locus_tag="PC407_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2497804.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(9204..10226)
                     /locus_tag="PC407_002362"
     CDS             complement(9204..10226)
                     /locus_tag="PC407_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDF2497805.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWICKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(10226..12646)
                     /locus_tag="PC407_002363"
     CDS             complement(10226..12646)
                     /locus_tag="PC407_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2497806.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLALLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKILINQGNLNTFGTNLVNDILSKTRHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSDSEIGDEDDYAVIETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(12732..13652)
                     /locus_tag="PC407_002364"
     CDS             complement(12732..13652)
                     /locus_tag="PC407_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2497807.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHFVDIYLPIEPF"
BASE COUNT         4962 a         1815 c         2218 g         5049 t
ORIGIN      
        1 gattataatt taaaaataag ctataaaaac ccaatattgt tttgttcaga gataaaaaag
       61 aatactttag gtgattttta ttaaaaatta agagtgataa gattatatac tttataaatg
      121 taaatatgac gaataaaaat gataaatata ataatctata tatttacatt aggagttttc
      181 tatactataa ttatttatat ttaaaactaa atataaatgt tggaattatt taggatttga
      241 aattgtctaa agataaatat aagtgatgaa gaggtgatgt gatgagatta gatatggata
      301 gtatgttata tgtactaatt tatatgacat tattggtatc tatcattgga agtatatttt
      361 atttttgtaa agacctctta aatcataaaa ttaagtatgt tttaataaga gacatatttg
      421 taaatagaat tacatattct gaagaacggt tgcctaaaca gtatatagtt tttcagaaat
      481 atgatatttg gaggtattgt agtttgtttt aatttgaaat gaatctatta taaattctaa
      541 agtttaaaaa taaaaaggag agaataaaat ggatttcatt tcaagtatat tgcaaggatt
      601 attaagtata ttatttaatt ttacaggtga ttttggaatt tctattgtac ttataacttt
      661 aatagttaaa ttagtactta tgccactatc attaaaacaa agattttctg taaaaaaaca
      721 gcaagaatta gctgaaaaaa tggaacatat aaaagaaaag tataagaatg atactaaaga
      781 attggaaaag caaatacaag taagttctgt agaaagtatg aagagtatgc tgggatgtct
      841 tatagttttg gttcaaatgc cagtagtata tgctttatat catgtatgtt tagatatgac
      901 aaaggaattt acaacaatta taattccatg gattgctaat ttaggtacat ttgataattt
      961 gtttatatta ccttgtatat atacattaat tatgttagct tcaaatttag tgaattatat
     1021 accatattta aaaataaatt ctcaagtaaa gtttaacaaa caaatggctg tggtgactac
     1081 cataacaagt cttatactaa ctgttagaac tccagtagcc ataggtctgt actttataac
     1141 aagtgcaatt tattcattaa ttgaagatat ttgttttaga atgtatttta atcgaaaaaa
     1201 tagaatagtg acaaaataat ataattatta ttagaaatat tttagcctgc taacatagaa
     1261 aaagttagta ggctattttt atctatgata tcaattccat taagaatagt ctaaaaaata
     1321 ctatcaaaat gctataaaaa atactataaa aatactataa aaaaatagtt gaaaaataaa
     1381 taaattaata taaactaaaa taaagcctac aaaaaagagg tgataaatag aaatggctaa
     1441 aatactagtt gtagaagatg aaaaaaggat gcaaagtatt attgtagaat atatgcaaaa
     1501 gggaggatat acttgtataa ctgctaatga tggagtcgag gcacttacaa tattaaagag
     1561 caacaatata gatttaatga tattggatat tatgatgccg tatttagatg gattttcagt
     1621 gtgtagagtt tcacgtgaaa tgactaatat acctattatc atattgacag ctaaaggtga
     1681 agaggaggac aaattaaagg gttatgaata tggcgcagat gattatataa caaaaccatt
     1741 tagcccaaaa gtacttttag caaaagttaa tgccttgtta cgtagataca ctactgatat
     1801 tcccaaaaat tcattgagtt taggaaagat ttttattatg gtagcatcaa ggcaagtgta
     1861 tgtagaagat aaattgattg atttaacata taaagaattt gaattattgc gtttatttat
     1921 ggaaaaccct aatcaagttt tttccagaga gaagctttta aattgtatat ggggatatga
     1981 ttttgaagga aacacacgaa ctgtagatac acatataaaa accttacgca aaaagcttgg
     2041 tagtgaagga caccatatag ttacattaat tcgttcagga tataagtttg aggttaagga
     2101 atgaatagtt taagaaagat tatagaaaaa ctgaaaaatg tagatatttc aaattggatt
     2161 aaacaaaatt ttggactata ttcagttaga aaaaaagtgt ttttactttc taaattatca
     2221 ggagcgttaa ttatattatt ttatctggtt gttgaagaat tttcgataaa ctctaaattt
     2281 gaattttgga tttggtttat aatactggtt atatttatta tgagtattga ttttttattg
     2341 ggaaaattta tttcagaacc aatcacatct ataaataaat ctgcaaaaag tatgtcccaa
     2401 cttgattttt ctaatccatg tactgtaaat actaatgatg aatttggaga actttctagg
     2461 agcctgaata caatgtctac aaatctacaa caggcattat cagacctaga aagtgcaaat
     2521 attcaacttg aaaaggatgt aaataaggaa agaatgctat tggaacaaag aaaggagttg
     2581 gtagatgcaa tttcacatga aatgaagact ccacttggca ttatacgtgc gtatacagag
     2641 gggcttatgg atgaagttga tgaagaaaaa aagaaaaatt atatgaatgt tataatagaa
     2701 gaaactgata gaatgaacaa tatgattgta tcattattag acttatctgc attggaagca
     2761 ggagtttcta aacttaaccc agagcgtttt gattttatag agtttgttga aactgtggca
     2821 ggtcgtcttt taattgatat accagatgcc aatttttatt ttacatatga tttgccagag
     2881 tatgaagttt ttgtagtggc agataaaatg agaatggaac aagtagtaga gaatctaatt
     2941 attaatgcaa agaagcatgt tgtatataat ggtaatttag atttatcgat tacatgtgaa
     3001 aatggcttgc ttttatttga aatttataat gatggtagga ttatagagca agatgagatt
     3061 tcaaagattt ggtctaagtt ttatcgtagt gttcaatctc agcgtaaagg aggttcaggt
     3121 ctagggcttg caattgtttc acaaatactt acaatgcaag ggttagaata tggagtgaat
     3181 aatcgtgata gaggagtaga gttttatttt atgattccaa ttgatgaata atttagtatt
     3241 aatttataaa ttataagtat ctgatgtaaa atatttttct ttgtatacgt aagtatttaa
     3301 agagaataaa ataaaaaact taatgaaaaa gtctaataaa aagtttaatg aaaagtcttg
     3361 ttttcctgat gaaaacaaga ttttttatta agctttttat tatttcactt cgatttcaca
     3421 agagagacac tctgatttca ccaaatagaa ataaaatagg ttaaataaat taaaaaatag
     3481 aagcgtaatt aaataaggtt acaaggggga gtacttatga aatggtatac atggttgact
     3541 attatattga taatattgct ttctattccg cttaaaatta aatttataaa atggtgggat
     3601 agacgacaaa aaaatgataa aggaggttat gatagatgat aaaagtagga gagttaaaat
     3661 tttcttatgg aaaagataaa cagatattgc atggacttaa ttttgaagta aaagaaggag
     3721 aaatctttgg attccttgga cctaatggtt caggaaaatc cacaacacag aaaatcttaa
     3781 atggcgtttt aaaaggttac aatgggcacg tatccctttt tgggaaggaa gttaaagcat
     3841 atacagagtc tctatatcaa aagattggtg ttttatttga atttccttat ttatatacca
     3901 atttaagtgc aattgataat ttagaatatt tttcgtcttt ttatcctaaa aatcaaagga
     3961 gagacatata tgaactctta gatttgttag agtttaagaa agagtttata aataagcctg
     4021 tatcatccta ctcaaaaggt atgaagcaac gtattagcat ggcaagagca ttggtaagta
     4081 atccaaagtt gttattttta gatgagccta ctagtggact tgacccatct ggagctgtac
     4141 tatttcgtaa gattattgaa gaagaacgaa aaaaaggaac aactatcttt ttgactactc
     4201 ataatatgct agatgcagat ttaatgtgta acaatgtggc ttttattgca gatggcaaga
     4261 ttatggtaat agataaaccc aaaaatttaa aaatgaaaaa tagcaataat aaagttgaag
     4321 tggaatttgt atataatgga aatcgagata ttgaaattgt agatatacaa gagttagaat
     4381 ctggaataac ttttaaatat gatgaaatct taagtgtaca ttcaaaagaa cctacactag
     4441 aagaagtttt tattaaatac acaggaagga tgttagtgta atgtattcta aattttttac
     4501 cttagtaaaa aaagattttt ggatgatgat ttatggtaaa ttttttgtgc tgacacttgg
     4561 ctctctcata ctttattcgt gttatattaa ttttggttat gtaaagttta tggatgaaga
     4621 tagttatcac aataatgtat atctatataa tccagagaat ataagcatga gtgcatctcc
     4681 attaattaat caagtttctt ctataggtga attaaaagca aaacttgatg aagatacaag
     4741 tgcagtttgt attgatatga gttccagtaa gccgaaagta attttacatg aaagtattaa
     4801 aggagttgac aattatagag ctgactatgc attgtcatta ttatcatcag aaaaagacca
     4861 taatagcaaa atatcagaca acaatgcaat aattcaaatt aatgataagg atacatctga
     4921 gataaaacaa agaaaagaca tgacttgtga gattttattc tttgaaattg tagctattgg
     4981 atttttaggg attgcctctt tattatttaa agaaaagcag atgggagtaa tgcgtattac
     5041 tggtatttta cctgtaaaaa gaagtctttt tatattatca aagatatttg cttttttgat
     5101 atgtgaccta tgttttacag ttcttttagt agtatttaat attggatttt ttgaaggagt
     5161 aaaaatactt ccacaagtat tagttcaagc tagtatttta tcaattatta tgtctttgat
     5221 tggaatagga tgctcaatgt tattgagaga ttttaagcaa ttttctctta tatatcttgt
     5281 ggttgctgta ttcataacta cacctatatt tctatctgca aatacatctt ttgaaataga
     5341 ttggattaaa tatcatccct tctatcatgt ttatagtcag cttaaaaatg cgtttttcat
     5401 gaatttttca aaagaattag tttattttgt agtgtgttct ttgacaataa tagttctatt
     5461 tttacttgta aaatatattt ttaataaaga gttggttagg gagggataat cttgatagga
     5521 ttacgttacc agttaaagag tataaaaaaa gataaaatgt gtataatttc cttcttttta
     5581 ccagttgtta tggcgatttt aataaatttt atgggaacaa ttgatatatc ttcattagga
     5641 gagatacgtt ttggtatagt gaaggataca cttcctgtac aaacagagca gtggttgaag
     5701 aagtatggta tggtaagtgt atatgacaca aaaatgcatt taatacgtga aattaaggat
     5761 attaaaacgg acacaattgg agttgttaga gatggtaaag gaataaaaac tatgctttat
     5821 ggaaatgaga ttaatgctat aaaaaatgtt ggaaagacat tgccagagat gtttagacta
     5881 aagggtgatt taaaagatgt tgaggttaca atattgccta aagatagtat tttaaaagag
     5941 tttcagaatg tttttattgc tatgatttta attactgcta tgtttatggg atgtaccttt
     6001 aatgcaatga atattatatc tgaaaaagaa gatggaatat catttataaa tgaagttttg
     6061 ccacagagta gaagtcagta tattatacaa aaagtatttt tgggtctttt atgtgggtgt
     6121 ttatcttcaa tgataactgt atttatatgt ttggatattt caatattaga tatgataatg
     6181 atgttgttac tcgttgtatt ttcagcattt atttcatcac ttataggttt atttattgga
     6241 aagttgtcag aaggtcttat ggtaggaatt gtttatatta aaatagtaat gattttattt
     6301 ataggtgctc ctttgttaat atatcttcta ggggttaata ccagcggtat agggaattta
     6361 tgctatataa tgccatcagt tgctacattt aatggtatta tggagattat taatggtaat
     6421 gttattatta taaaagatat tgtaattttg gttgtacatt gtgtagtatg gtttatgata
     6481 tatattattt tagataaaaa aagaaagtta tcttttagta gataactcag tataattata
     6541 attgggacaa tttaataggt gatttaatat actttgtata gtgtaattta tgtagtgcaa
     6601 tttgtatact ttaatttatg tagtgtaata gttagtttta ttttataatt attcaatata
     6661 atttagtaag aaattcagta caattagagc tacttagtgg aaaacttaat aggtacttaa
     6721 actatataaa tactttattt tcctccattt gtactataaa tttaatttat gatataaatg
     6781 gaggtattat tatatgaggt attattatat aaagttaata aagatagtat caaaatatta
     6841 tttgctataa agagagggac tcaaaataaa gataaaccta tttttcaatg ttgaaatgat
     6901 aaaatatatt tgatttcata taaaaaattt tcttcataaa ttaaaaagtc cattttaagc
     6961 cccctcattt ttaaccagat aaatcgagtt aaactattta aatatattac tcggattcat
     7021 ttgacttaga tattccttgc ttgtaaaatt gttttgaata ggggatagcc aaagcaaaaa
     7081 ataaaagtat aatccctgct acgattatta aattattaat agaaattaca tctgccattg
     7141 gtccgaaaat catcatacca agaggaataa aaccagtata tataataata ggtaagctaa
     7201 gaactcgacc tagcatttgt ggtgctacat gctcttgcaa catagtcata gctgatgctt
     7261 gaataattgg aattgaaaaa ttcgtaaaaa acataaatat aaaaaattgc caagcttgtg
     7321 ttacaagacc tagaataatt gaaaaaatag cataggataa tattgctaga aaaaaggtct
     7381 tatttctatt tttaaaacca ccaaaaactc ctataatcaa tccacctaaa accataacta
     7441 aaaatccaat catttcaata atagacaggt atatataacc cttgccaaat gttcgttcaa
     7501 taagaagtcc tataagaaat cctgatggaa cacaaagaag catgaatgct ccataagtga
     7561 aaagcacttt tctaatgaac ttatctgaaa aacagtactt tatccctgat accatatctg
     7621 caaaaaatga gactttttca gtttgttttg gtatattatg tttaggaatt ttgatgaata
     7681 tcaataatac aatagcaata attgcagtaa atacatcaat taaaagaact tgatttattt
     7741 tattaccaaa tccaagtatt gcagcagcta caataggcac tataaattgt actatagact
     7801 gtagactact atttatacca ttaaaacgca ttaaatgttc ttctggtaca agttgaggaa
     7861 tcattgcgtt tacagcaggt gtttgtacac cacttcctag tgaacgtata acagatatta
     7921 ctgatatggc tattaattca cttgttgtgc catttgatga catcataaat agaattagaa
     7981 ctaatgtaga tatagcaatt gttgaatctg atacaattat gagtaccttt cgattgtagt
     8041 agtctgccca tacaccagca aaaaaagaaa ttatcatttg aggtataaag gagcatacag
     8101 ttaaaactgt tacccataca cctgatgatg tctgaagtgt gacataccat atcattgcca
     8161 tttgtacaag tgatgagcca aataaggaaa tatattgact tgctaaaaaa agaatgactt
     8221 ttttcttcca tgtagaatta gtatttttgt tttccatcct attcctcctt tgatttgtgt
     8281 gttgaatttt tcttgctctt aaggataaca cacaaatcaa agtctggagt tagagtggag
     8341 ggctaggaaa attgtttttt cataatttga agatgagatt tcatggtttt tgcattatgt
     8401 tcagcttctt tcaatgaagt tatcagtttg tcacacactg agataatttc gtcatcatct
     8461 tttggagtgt caaggacagt tttaatatta gtattaggat tagaagataa agcatttaac
     8521 attcgtaaaa tagctgaaag agaatagttt gcacaacgaa gtgcgcttat aattttaagt
     8581 cgttttatat cttcatcagt atatacacga taaccatttt gttttctttt tacttttaac
     8641 aaaccattca tttcccaatt tcttaaagta tctattgtaa catttaagtg ttctgcagtt
     8701 tcttttctag ttaaaaagag tttatttgtt tctggagtat ttccatttaa aatctgtgca
     8761 acaatagtaa gtgcttcctc ggcattagat ttttctattt ctaattgttg caagtattga
     8821 tttacaagac taatagcctt atcaaagtcc ctattcgctg atgttcttac aatctcaagg
     8881 gcatcttttc ttaatcccct ttgtaccact tcaattttca aggctgtacg agcaaatcta
     8941 aattgttcta tatgaaaatc attaaagata cgataaccat tggtattacg cttaggttta
     9001 ggtattaagt ttagttcttc atataaacgt actgtattag gatgtacacc aattaaatct
     9061 gctacctctg atgtcgtgta agtgtccatt agtattcaac tccttttaga tatatagtat
     9121 tagtcataat aacaccaaac aaaagtctgg tgttatagtg gagggtttgg ttatctaaga
     9181 gtaattagtt tatctaagag tgattaatct attatagaag taagtatata tttttcaata
     9241 aactcccatc tatcacgagg tttaaacact attttagatg gtatggctat tactaagtca
     9301 tattgtggaa tacaacaaat agcatttcct ccagttccca ttgctgaata ccacaaaaca
     9361 ccatcttttt tacatatcca ccacagataa ccgtattcat tactattcat tttaaccgat
     9421 tcttcaatcc acgatttaga tataatacgt ttattattcc aaataccatc atttaaatac
     9481 agaaaaccaa atcgcaacat atctctagca ctcaatgtaa gtccccagcc acctatagaa
     9541 taaccctgtc tatcttttgc ccaaccctta agattttttc caaagaaatc ttcaaaacca
     9601 aaatctttca tatcataatc tggtataact ttcataccaa taggtttaaa taagtactca
     9661 ttagcaaatt cacgagcaga ttttccagta gtttttgtta taatagctga aagtaggtgc
     9721 gttccagctg aagaatattt gaaagtacca atttcgcctt tttgaccaag ttgattcaag
     9781 gtatatttta tccaatcttt ttgtttgcaa agtctttcta aagactcagt catattttta
     9841 aaagggtatg gtgcagtcat tgtaagtagg tgacgtatag ttactgcttt tttacatatt
     9901 tcagaaggac tacaagtata ttcagggaaa aaatcaacaa ccttttggtc aacatctttt
     9961 atatatcctt tatctattgc aatacctatt aaggctgata taatactttt tgtaactgat
    10021 gcaacatgaa aagtatcatc agcattatat ccattatagt acttttcaaa gacaactttt
    10081 ccttttcttg caataattat tccatttata ttactatatt gtgagattat taaaggttcc
    10141 agttgtgaga gcttttctga actcatgcca acacttgaag ggtctacttt ttcaaatatt
    10201 aaatctaagc aattgttttt attcattaaa gatacctccc tgttatagat tttggatttt
    10261 cttttagagt ttttggagaa ccttctgcaa ttatataacc tcctttgtca ccaccttctg
    10321 gaccaagctc aacaatccag tcacaaacat ttaatacatc tatatcatgt tcaacaacaa
    10381 ttacagaatt tccattttcc accaattcat ctaataattg aatcaactta gaagtatcat
    10441 atagacttag accagtagtt ggctcatcca atacataaag gatattacca tttcgttttt
    10501 taccaatttc ttttgcaagt ttgattcttt gggattcacc accacttaaa gtcggtgtag
    10561 gttggcctaa ttttatatac cccataccta tttgttctaa tacatttaag tgtgaaacaa
    10621 tatttggaat atccttgaag aaatgtacag attcagaaac actcatattt aaaatatcat
    10681 gtatattttt atttttatat ttaatagata aagattcatc attaaatctt tttccctgac
    10741 attcactgca tatatggtct atacttagat tatttcctaa ccagaccttt tcatatccac
    10801 taccactaca tttaggacaa gcacctttgg agttaaatga gaaatgacca gctgtaaatc
    10861 ctcgttctaa cgattctggc tgacctgcaa atagtgtacg tatcttatcc catataccaa
    10921 tataagaagc aggagttgag ttcatatttt tgccaatagg agattgagat atctctgaat
    10981 atccagaaat atgtttagta ccaatcagtc tatctgcaat agtttcaata actgcataat
    11041 cgtcttcatc acctatttcg ctatctgaag aatttatttg attattgata gtatggttat
    11101 taaattggct tctaagaaga ggaagtaggg tgtctgatat aagtgaactc ttgccactac
    11161 ctgattttcc agatattcca accatagcac ccaaaggtaa tgatacagtt acatttttaa
    11221 gattatttgt atttgcattt tgaatagtta tacaaggtat tttatcagta tgtgtaatat
    11281 ttttattgct tgttctatta ggcattggat attttccaga tagatactgc ccagtaagtg
    11341 atttgtcgga ttgaagtaga ccttctaaat ctccttgata tactaattga ccaccttcaa
    11401 caccagcttt tggaccaata tcaataatat gttctgccat ttcaattgtg tttttatcgt
    11461 gctcaactac tatgaccgta ttgcctaaat ctttaagttt ctttattgat tttaagatgt
    11521 ctattttttc tgattcatgc aatccagctg ttggttcatc aagcacataa attaatgagt
    11581 ccatctctga atcgagatgt gaatttaaaa atagtctttg catttcccca ccactcaaag
    11641 ttgacatttc tcggtataag gatagatgac ctaatcgtga ttctattaaa tgtcgagttt
    11701 tggataaaat atcatttaca agatttgtac caaatgtgtt taaattacct tggtttatta
    11761 agattttaag gaaatcatca atttctaaaa tcgtcatttt tccaagttct ccaatatgtt
    11821 ttccgtttaa aagtactcta cgtgcttctt caccaatttt aaaaccattg caatctgaac
    11881 aggcagtcat agtataaact ccatttaaat cctcacccct tttgtaacga ttgtgaaata
    11941 cctttgataa agaggaactt tttttaccat tttcataatg accatagagt acttcatttc
    12001 ttatttcttc aggtagttgt aagaattgta tgtctaaata atcgttgaat tttttagaaa
    12061 atactctcaa atatcctggt gtaatctttg ctctatctaa gacttgtttt agggtatcat
    12121 gcttatcaag gactaatttt tcaatattaa tctcaaaata tgcaccatgt ccagagcact
    12181 tcatacacat tccatctgaa gagttgtatg agaaataact tggtcttaat cgttcttcct
    12241 catacccaca attattgcaa attagattat cgcttacagg agtatcacac atagtacaca
    12301 ttatttgacc ttctactgaa tacaatagtg caagcatgtt gagaatattg gttcttgtgc
    12361 ctactgttga acgaggattg ctctggcgaa taatattttg ttgaacagct atagtgggag
    12421 ctaaaccttg aatattatca aacttatcct cactatcaat acctgcaagt atacctaaag
    12481 attgcagata ttgtttacga ccttcctcaa atacaatgtc aaacataagg ctagacttac
    12541 ctgagccact gacacctgtt gcaacaacaa gcttatcttt ggggatagaa acatctatat
    12601 ttttaagatt gtgtatcttt gctccattaa tttttatttc tttcattggt tcacctcatt
    12661 ttatcaattt tatcaaaccg taaaataggg atatattaat ccttaataca agaatagatt
    12721 aggtgaggtt attaaaaagg ttctataggt aaatatatat cgacaaagtg tctattttga
    12781 gggtgattgt gtgggttgtt aagatatact tcaaaaggga aggaggcacg tagcttataa
    12841 ttaccatttg atagccattc attatacata aaatcccacg catcactata ttcagatttt
    12901 gaaatatcaa aatgtcccac aagatatttt ccagaaggga ttatcatatt gccaagattg
    12961 tcattctctt tgacaacaat gttatctgga atggtcatgc aaatactagt ccttaattga
    13021 tgctcctgtg taaattgtgg attatcatga taaatggcta atatttgtgt gttttcaagt
    13081 ccaagtagat tctgctcact tgcatatgtg aataaccgtt ctaaaagttt tgggaaagta
    13141 ctggttagat taccatatga accagtatat ctagtgtaaa tagtattcat attatcaagt
    13201 tctagaattt caatttcacc tttaacctct ctagtattct tacatttagg ttttgatatg
    13261 cttctattgt actgagaaat tttatatggt tctttgcaat tcttgctata attgtttcta
    13321 tattttatag ggcttatttt atagtaattt ttaaatgctc tcgagaaaac tgctgaatca
    13381 gtaaagccga agtagtgtgc aatatcagta acagtcatgt cagttcgatg aataagaaaa
    13441 gcagttgcat tttctagttt tagcctattt acatattgtg ccaaagtttc tttttctatt
    13501 gctttgaaga ttctatgaaa atgaaatttt gaaaaacctg ctatattaga aagttcatca
    13561 agagaaagag gttcatgtaa atgttcttct atatagtctt gcactttgca aatacgattt
    13621 agatattctt ttttactttt agcatttgtc atatatagcc ctccttattg tgtaaaatgt
    13681 gtattaatta atttatagca atatagtaat aaaattttat gtttttgcaa tttatgataa
    13741 ataagatagc gtttaatttt ttataattga taaaagatat ttataattaa taaaagatat
    13801 aagatgcctt atatttattt ttgcatataa gtatagtctt cgtaataatt agtataccat
    13861 agtgatttat ttttaattgt gtatttatat aaatacacaa cttatgatat ggacgaatta
    13921 ttatgcaaat aagctgtaat taaataaatt gcaatataaa ccagttgaaa cataagttag
    13981 ggaaatatta tcaaagttat tatttagcat aagagtatag agggaataaa ataagattaa
    14041 aaaa
//
LOCUS       DAMJVX010000092        14026 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMJVX010000092 DAMJVX010000000
VERSION     DAMJVX010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.096"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            419..1312
                     /locus_tag="PC407_002365"
     CDS             419..1312
                     /locus_tag="PC407_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HDF2497808.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     ILKLEEGDSHHHSNCGCGGNK"
     gene            1312..2706
                     /gene="gltA"
                     /locus_tag="PC407_002366"
     CDS             1312..2706
                     /gene="gltA"
                     /locus_tag="PC407_002366"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HDF2497809.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLNINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKTKVNA"
     gene            3072..5291
                     /locus_tag="PC407_002367"
     CDS             3072..5291
                     /locus_tag="PC407_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2497810.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLRPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5438..6184
                     /locus_tag="PC407_002368"
     CDS             5438..6184
                     /locus_tag="PC407_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2497811.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PTILLADEPTGNLDSKSTDDVLKLFTRINKEQNVTTLMVTHEVYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6184..8064
                     /locus_tag="PC407_002369"
     CDS             6184..8064
                     /locus_tag="PC407_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2497812.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELTILGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEIYFPVKAILLTIVS
                     FMILFLITGPMSLKLLRKKSILELLVGTKKPKKEIKSSRLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYDEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFRLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            8183..9574
                     /locus_tag="PC407_002370"
     CDS             8183..9574
                     /locus_tag="PC407_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2497813.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9681..10721
                     /locus_tag="PC407_002371"
     CDS             9681..10721
                     /locus_tag="PC407_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2497814.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11210..11863
                     /locus_tag="PC407_002372"
     CDS             11210..11863
                     /locus_tag="PC407_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2497815.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIHGIIDGMKF"
     gene            11939..12148
                     /locus_tag="PC407_002373"
     CDS             11939..12148
                     /locus_tag="PC407_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497816.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12288..12854)
                     /locus_tag="PC407_002374"
     CDS             complement(12288..12854)
                     /locus_tag="PC407_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDF2497817.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSP
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            13015..13668
                     /locus_tag="PC407_002375"
     CDS             13015..13668
                     /locus_tag="PC407_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDF2497818.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13826..14002)
                     /locus_tag="PC407_002376"
     CDS             complement(13826..14002)
                     /locus_tag="PC407_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497819.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         5500 a         1385 c         2366 g         4775 t
ORIGIN      
        1 ggtattgcaa tagattaaag ccttatacac gtttagtttt taaagaaatt agtgttatta
       61 tgaaaaccta agttaattta ttaaataaat aattgaataa aaaaatttta ttatgtattt
      121 ataaaatttt tgttacaaat ttggcataat atagtatact ttaagtgtgt attaggttaa
      181 atctataaca aattagacgt atgatataga cttagtgttt attggcattt acaagaaatt
      241 tgttaatatc aactatttgt agtttgggca gttataatta ctataggggg gatgtccaat
      301 acaaaacaat caaccaagaa ctaatattat attataaaga gttatagttt tgtgtgttta
      361 tgtttttaat taaatttaaa gtgggaaaaa tatatttgtt agaatggaga atagcgagat
      421 gagtaataaa atagttagta agagacaatt aacagatagt atttatttaa tggaaataga
      481 ggcacctaga gttgcaaaat cttctcaacc aggtcaattt ataataatta aaaatgatga
      541 aaagggagag aggattcctc ttacaatagc tgactatgat agagaaaaag gaacagtaac
      601 tattgtattc caaacagttg gtgcttcaac taaaaagtta gcaatgtttg aagaaaacga
      661 ttttgtaatg gattttgtag ggccattagg acaagcaagt gaatttatac atgaagatat
      721 agaagaatta agaaataaaa agatattatt tgtagctggt ggagttggtt ctgctccagt
      781 ttatccacaa gttaaatggt ttaaagagca tggattagat gtagatgtaa taattggtgc
      841 aagaactaaa gaattaatta tattagaaga tgatatgaaa aaagtagcta aaaatgtata
      901 tgtatcaaca gatgatggta catatggatt taatggaaga gttacagacc tattaaaaga
      961 tttagttgat aatcaaggta aaaaatatga ccaagcaata gtaattggac caatgataat
     1021 gatgaaattt atgtgccaat taactaaaga actaaatata cctactatag taagtttaaa
     1081 tacaataatg atagatggaa caggtatgtg tggtggttgt agagttagtg ttggaaatga
     1141 aactaagttt gcatgtgtag atggtcctga atttgatggt catttagttg attttgatca
     1201 agcaatgaga agacagtcta tgtacaagac tcaagaggga agagctattc taaaacttga
     1261 agaaggcgat agtcatcatc atagtaattg tggatgtgga ggtaacaaat aatggatgct
     1321 aaaaaagtaa aagtaccagt tagagaacaa gaacctgctg tgagagcaac aaattttgat
     1381 gaagtatgct tagggtataa taaagaagaa gctatggcag aagctaatag atgtttagct
     1441 tgtaaaaaac caaaatgtgt gggtggatgt ccagtaggaa tcgacatacc agggtttata
     1501 acaaaaatta aagaagatga tatagaaggt gctgctaaag taatagctaa gagcagttca
     1561 ttaccagctg tttgtggtag agtatgccca caagaaagtc aatgtgaagg tgtatgtata
     1621 cttggaataa aatcagatgc tgtatcaata ggtaaattag aaagatttgt agcagattgg
     1681 tcaaaagaaa atgacataaa tttatctgat acagagccta agaaaaatca aaaagtagca
     1741 gtaataggaa gtggtccagc aggtctagct tgtgctggtg atttagctaa aaaaggctat
     1801 gatgtaacaa tctttgaagc aatgcatgag ccaggtggag ttttaactta cggaatacct
     1861 gagtttagac ttccaaaaca agctgttgtt caacctgaaa tagacaatat aagaaaatta
     1921 ggtgtaaaaa tagaaactaa tgtaattgtt ggtaaaacaa ttacagtaga tgaacttata
     1981 gaagatgaag gatttgaagc tatattcata ggttcaggag ctggtcttcc aatgtttatg
     2041 aatataccag gggaaaatgc aaatggagtg ttttctgcaa atgagttttt aactagagta
     2101 aacttaatga aggcttatag agatgactat gatacaccta ttagttctgg taaaaaagtt
     2161 gctgtagtag gtggtggaaa tgttgctatg gacgctgcta gaactgcatt aagattaggt
     2221 tcagaatcat atatagttta tagaagaagt gaaaaagagc ttccagcaag agctgaagaa
     2281 gttcatcatg caaaagaaga aggcattata tttaatacat taacaaatcc taaggaaatt
     2341 ttagtagatg aaaatggata tgttaaaggt atggtttgta taagaatgga attaggagag
     2401 ccagatgatt ctggaagaag aagacctata gaaatagaag gttctgaatt tgtacttgat
     2461 gtagacacag ttataatgtc acttggaaca agcccaaatc cactaatatc ttctacaaca
     2521 aagagtctta atataaataa gaagagatgc ttaataacag atgaaaatgg tcagacttct
     2581 aaagagggtg tatttgcagg tggagatgct gttactggag cagcaacagt aatatctgca
     2641 atgggtgctg gtaaaacagc agcagcatca atagatgaat atttaaaaac aaaagttaat
     2701 gcttaatttg taaattaaag agagggtgtc tcaaaatgaa ctttttagtt catgaggcac
     2761 tttttttgta caaaatcaaa tatgttttaa tcatttcaac aatgaaaatt tcattcctgt
     2821 agaaagtcta acttttaagt cttagtacaa tctctatttt gatgtaggca cttttttaga
     2881 tggtttaata atttatcagt tattattgta atatttgata ctaagtaaca taactttgtc
     2941 taactatgat aaaatagtta tacaataaag gtcttgtaag gagtgaataa tgtgtggcta
     3001 ataaaatcag atttgtcaaa tctaacatta aataatagaa aaataaattg tgtaattaat
     3061 ggtattttaa tatgaaaaaa agagaaaaag tatttgatgg gctagttttt aagcatatat
     3121 taatcttatt aatactatta gttttgattg atttaacaag taccatcata tatgcaagta
     3181 actctatgga aattacttct agaaatatga ttgaggcatc aaaaagagaa cttgaaaact
     3241 atttagaagt aaacatatct ttattaaaag ctcttagtca agatgataga ttttctaatg
     3301 gtgaaacatc actaattgaa aagggaaaat tattaaggcc atatcaaaag gaatataatc
     3361 tttttatgat tggtataaca gatacaaaag gaaatgcttc aagtacatat agggaaaatg
     3421 ttaactcaat aaaagataag cctagttttg agaaagcaat taaaacaaag caagtagtag
     3481 tttcagatat tgaagtcagt aatgtaacag gtgacaaagt atttatcata tatgtaccaa
     3541 ttgtaaaaaa taatgagatg attgggacca tttttgcttc tttttatttt caagatgtaa
     3601 ataatttaat aagtagaagc aattttgatg attctattaa atttctaatg atagataaag
     3661 attatactat tatttctcat ccaaacaaaa aatatgtaaa tgataaaagt aagatgcttg
     3721 atttagaagg gaatattata ggaaccacta aaagtgaaat cttaaaaaat ataaataaaa
     3781 aatgtcaagg tgatttttta tcttgggata attggagatt atataatgta aaatatacga
     3841 acataaagtg gactaattgg actttagtat caaaatgtaa tatatttaag aactttaaaa
     3901 atctaatagt taattttatg ataaagttgt atttttatat agtaatattt atgatattgt
     3961 ggaaattaag taatgctaaa ttaatagaac aacttaaaaa attggcttat tatgattctt
     4021 taagtggaat aaaaaataaa gaaaagttta gaaaagattc tatgtatatt ttaaagaatt
     4081 attatcaaga taatttttac ttagtacaat tggatgtcaa taaatttaaa tatataaatg
     4141 agatgtttgg atatgctgaa gggaataaga tattaattca tatatcacag gttttaaata
     4201 ataatacaaa taaatatgaa atttgtgcta ggatggataa tgaccatttt atattgttga
     4261 ttgcttgtaa tactgaagat gaacttttaa atagattatc taaaataaat aaagaaattt
     4321 gtaacttaaa tactactaac tcatctaagt ataaaattgt tatgtcaagc ggaatatata
     4381 agattactaa aaaggatgat attaaaaaga ttgacctgtt aattgataga gcaaatattg
     4441 cagcaaaaag taaaaaggaa aaatatgaac attcatattc gttttttaat gaagatacaa
     4501 gaaatcgatt gtacaaggaa aaacgattgg aagataatat gaataaagct ttagaaaaag
     4561 gtgaatttat agtatattat caacctaagt atagtttaga tgatgtaaat gaaattgaag
     4621 gagcagaagc cttgataaga tggaatagtc cagaatttgg gtttatttca cctatagatt
     4681 ttgtaccttt atttgaaaag aatggtttta ttgtaaatat tgatatgttc gtgtttgaag
     4741 aagtatgcaa aacattaaac aagtggataa ataaagggta tactcctgta ccaatctctg
     4801 taaatatgtc tagagttcat ttatataggg ataattttat agaaaatata actgatttga
     4861 taagtaaata caatatatct cctgagttta ttgaacttga actaacagaa agtgttgtgt
     4921 ttgataattt aaatatattg attgatataa tgaaaaagat taaagagata ggattcctta
     4981 tatcaatgga tgattttggt tcgggatatt catctttaaa tctgttaaaa gacctttctt
     5041 ttgatatttt aaaattagat agaggatttt taatcgaaac aacagataca aaacgtggaa
     5101 aaattattat ttctaagatt gtggaaatgg caaaagcgat tgatattaaa gtaatctgtg
     5161 agggcgtaga aacttatgaa caagtagagt tcttgaaaga gataggttgt gataaggttc
     5221 aaggatactt atttgctaaa cctatggtat tagatgaatt tgaaaaacac ttaaacttta
     5281 aatttgatta atcattaata attattagac tataatatac cctaacaact tacttacaaa
     5341 tatgtaagtt aattgttagg gtatttttat ggttaaagta gtatattcac gatattatgg
     5401 tattgtagta aaaataaaaa attatggaga atataaaatg aacatattga gtattagaaa
     5461 tatatcaaaa acatattgcg gaaatattcc atttaaagca ttagataagg ttagtctaaa
     5521 tatagaaaag ggagaatttg tgtctgttat gggaccatct ggaagtggta aatctactct
     5581 tttaaatata atatctacag ttgatagaca gagtgaaggt gaggttgtat tagatggata
     5641 tgatgtaagt aaattgaaag gagaaaaact tgctgaattt agaaggaaac aacttggttt
     5701 tgtatttcag gattttaatt tgattgacac tttaactgtt ggcgaaaata ttatgttgcc
     5761 acttacacta gagggggcaa gtatcaaaga tatgaatata caaactaaaa gtatatctaa
     5821 atttttagga atagataaaa ttttagatag aaaaacctat gaaatttcag gaggtcaagc
     5881 tcaaaggtgt gctattgcta gagctattat aaataaacct accatacttc ttgcagatga
     5941 gccaactgga aatcttgatt caaaatctac tgatgatgtt ttgaaattat ttactaggat
     6001 aaacaaagaa caaaatgtaa caacacttat ggtaactcat gaagtatata gtgcaagtta
     6061 ttcagataga gtaattttta taaaagatgg ttgtatatat acagaaataa aaaaatctga
     6121 aagcagtaat tctttctatt ctgatatact agctgttttg tctcaaatag gaggtgtaag
     6181 atagtggatt ttagaaagtt tgcttataat aatatagtta gaaatttcag ggcatattta
     6241 gcatattttt taagttctgt tttttctata atgatatttt ttgtttttgc agtgtctatg
     6301 ttccatccga ttataacaaa atctggaatg cagagtggtt ctactgcatt tatggctctt
     6361 atgacttcag aattaacaat attaggtttt agtctacttt ttatattgta ctctttagga
     6421 aatttcttga aatatagatt gaaagatttt ggagtattga tgataattgg aatgagcaac
     6481 agacaactga aaaaattgat attaattgaa aatttgataa taggattttt agcagtgatg
     6541 ttaggaatat tacttggttt atcaatttca aaattgtttt tattgtattt aagcaaactg
     6601 ttttatatga atttaactga aatttatttt ccagtaaaag caatactatt gactattgta
     6661 tcatttatga ttttattttt gataacagga ccaatgagct taaaattatt gagaaaaaag
     6721 agtattttag agcttttagt aggtacaaaa aagcctaaaa aagagataaa atcttcaagg
     6781 ctatttggaa taattggtgt aatatgtctt atattaggat atgctatgat actatttggc
     6841 aataagttaa aaataaatgg aggtatgtat acaacagcac tattaattac tttggggata
     6901 tttttatttt tttctcaatt tactattttg atattaaagt atttaaaaaa taaagaaaaa
     6961 ttttataaga acaaagtaaa tatgttactt ataagtaatt tgatatataa attaaaggat
     7021 aatacacgat tgttattttt aataacaata cttttatcgg ctactttagt agcatttaca
     7081 acaacttcaa cacttgtaag ttctcaagga gaagcttcta aaaataattt cccaatggtt
     7141 tactcatatt tttcagagca taataatgaa aaagaatatg aagaactaca acaaattaga
     7201 gaagcaatca aaggatatga ttataaggag ttaagttttc ctgtacttaa atataatagg
     7261 gaagtcttac taagtgtaaa gaattataac aaattatcaa aggaattggg attagataaa
     7321 attagttcaa agttagaaaa atatgaaggg attataattc ctagtagcaa ttcacaagaa
     7381 catataaatt ctttaaaaca attaaaaaat tatagtataa aaaatttaaa tatagaagta
     7441 aaaaaatcaa aaaataaaat aatttttcct acaggactgt ttagtaaagt tgtagttata
     7501 agtgatgact tatacgatga agtaaaaggt gaattttcaa aaattaattt tcatggattt
     7561 gattataaaa actggcaaag cagtgcaaag ataactgaaa atttaaaaga aaaacatttt
     7621 aatttggata gagaatataa caggtcatat tttttaacat taccagatat gtatttagta
     7681 gagttacaac aaagtaaaat tcttcaattt atgggtgttt ttataggagt gatattattt
     7741 atagcagttt ttagtttttt atacttcaga ttatatacag acgagataga agataatgta
     7801 aagtacaaaa aactatcaaa aataggtcta tcttttaaaa atatgaataa gattgttagt
     7861 atagagatag gtacattgtt ttttataccg tattttatag caattataaa tagcataatt
     7921 tctttactgt ttttaaatca agtattaaat aatagtttta ggttagaaaa tttgggtata
     7981 ttactactga tatcatctat atattcactt tatttttact tattaaaaaa aatttatact
     8041 agaaaagttt ggttttttga ttaaagtggt atataataga tatataaatt aatatttaaa
     8101 aagtagttaa tattagggga gggcatttgc acctcccttg taatatgtgg aaaattgaat
     8161 attagaagaa ggaggagact tgatggaaaa tcaacaatta ttaggaacag aacgaattag
     8221 taaattacta ctaaaatatt ctataccagc tataataggt atgttggtaa atagtttata
     8281 taatgtagta gatagaattt ttattggaaa tataccaggg gttggaccac ttgcaataac
     8341 aggtcttggt gtaacaatgc caataatgac aataatactt gcatttggga tgttgatagg
     8401 tataggaact actactacca tatcaataaa acttgggcaa ggtaaagtag aagaggcaag
     8461 aaaacttata ggaaatgcaa tgacgttatc tgtaataaca ggaattatca taatgatact
     8521 tggaatatta tttgcaaata aaatacttac attgtttgga gcaagtgaaa atactttaat
     8581 atatgcaaaa tcatatataa atattatatt attaggtaca gtagtaaatc ttctttcctt
     8641 ttctttaaac cactcaataa gagctgatgg tagtcctaaa atttcagcag gaataatgat
     8701 agtaggatgt ttaactaata tagttttaga ctggatatta atatttggat tcaatttggg
     8761 aatacaagga gctgcaattg ctacagtaac ttctcaggct cttacagcta ttttaactat
     8821 agggtattat ataagtggaa aatcaaactt acgattcagt aaatctaatc taaaattaga
     8881 taaaaagcta ataaaagcag tgtttgcaat aggtatgtcg ccatttgcga tgcaacttgc
     8941 agcaagttta gttcaagtaa tatcaaatat tgcacttaaa actcatggtg gagatttagc
     9001 tataggggct atggctacta tttcttccat tgcaatggta tttttgatgc ctatctttgg
     9061 aataaatcaa ggagcgcaac ctattatagg tttcaattat ggagctgaaa aatatgatag
     9121 ggttaaaaaa gcatatttag gttcattagt agtagctact ataattttat gtatgggtat
     9181 ggtagtagtt atgcttttcc cagaagctat tataggtata tttaataaag accctgaact
     9241 tatgaatata tctgttaatg gattaagaat atacttactt atgttgccaa ttgtaggctt
     9301 atcagttaca ggaacaaact ttatacaatc tataggaaaa gcaaaaatgg ctatgttatt
     9361 gagcctttta agacaagtaa ttttattgat accagcagta ttaattcttc ctacattctt
     9421 aggtttacag ggtgtatgga cagctcaacc agtgtctgat tttatagcta cagtaattac
     9481 aggtatagtt gtatttagag aattaaaaag atatactcct aaaactgaaa aattaaatga
     9541 aaatgaaaga ctaaatgaga taactactga gtaaaaatat aaattcagat aattgagcaa
     9601 gaaattgcaa aaaatacgat tatatgataa aataaaaccg ctgtcaagga taagagaaat
     9661 tctaattatg gagtgatttt atggaattta caaagaaaag caaattaaaa aatatgtatg
     9721 taaacatgaa tgtagctaga atgttgtcta agataaatga gtataagggt aggcaactgc
     9781 tttacaaaaa acaaccaaaa gaaattttag aaaacctaga aaaaaaatct ttagtagatt
     9841 gttctgaatc tacatacatt ggaaatcaaa gagatagtag taattttaat ctagaaaagt
     9901 tgataagtaa tgaagttaca cctagaagta gagaggaatt gagtattgtt gaatacagag
     9961 atgtggtaaa aactattaac agtgcttatg agagtattcc catcagttca caaacgattc
    10021 ttgagcttca tgggtactta tataaatttt catctactag aggaggaagt tataagtcag
    10081 ataatgattt tattgaacac aatttaaaac ctagcgaatt tataagtatt gattcaagtg
    10141 tcaataaagt tgaaaaagct gtagaagaaa tatgtgaagc ttataataca ttgatagagg
    10201 aagatgaaat tgatatatta atattaattt ctgcatttgt gttagatttc atattgatac
    10261 atccattcaa agaaggtaat ataaaaatgg cgagagtact tatcctattg ttactaaata
    10321 aaaatgggta tgaagttgga agatatataa gtttaggaaa gatatttgat gatagttcat
    10381 atgaatatta tagtaattta aattatttga aagcctcaat aggtagtgaa aaagcagata
    10441 tgaatgcatg gattgagtat tttttagaga cgatattaac tgcatatgag aagctggatg
    10501 atagtttaaa tatatctgat aagaaaagac aaacaaaaac tagtagaata gaaaaaataa
    10561 taaattctac ccttggatat tttacaaaag aagatataag agatttatgt cctgacattc
    10621 cagaacctac tataaataga gtttttaata atctaagaaa acaagataag atagaagttg
    10681 ttgcaagagg aagaagtgca aaatggaaga aaaaatatta aaaaatgttt aaataactaa
    10741 aatggtatag tgttttacac tataccattt ttaatgtcat tagcatgagc aataatttaa
    10801 caacttaagc aatatcaatt ttatttattg attatttttg aactgaaaaa tggggttata
    10861 tggtatacta caataataat taattttgga tgagaatata taacgttaca gaatatctta
    10921 ggagtatatg tataaaaaat ataataaaag tgtgaggttt agtaattaac aaattatgtg
    10981 gcttcacact tttttataaa ataaaaatac ttagcagaag aatattttag ttgatatgtt
    11041 tataagatag tacttgtttg ggtaaaagta taaatagtaa aaaagtagaa gtaatgtgta
    11101 taaaaacata agttacattc tgtaataata gttattttat aattcaacaa aagaatagaa
    11161 tgggtatatt tgtgaaataa taatataaaa taatcttagg agtgatctta tgaagaaaaa
    11221 attattatat ataaatgtaa attcaaaacc tgaagactta tcttcaagta aaactgtagc
    11281 aagaaaattt ataaataaat ttatggaaaa aaacaaagat tttgaggttg aagagataga
    11341 cctttataaa gaacatatac cgagacttga gtatcagtac tttgaaaaaa gaaatagcat
    11401 agttagtgaa gaaaatgcta aaaatttaga cgataaggat agaaaagagc ttacaaagat
    11461 tagaaatctt tgtgaccaat ttgtgagtgc aagtgtttat gtaatagcag cacctatgtg
    11521 gagtttatct tttccagcac cacttaaaga gtatatagat tgtataattc aagatggaaa
    11581 aactatatct tttgaaggca atgataaacc acaaggaatt ttaaatgata ttgatagaag
    11641 tatggtatat atacaatctt ctggtggaca cataccatgg gtcttgaagc cagttatgaa
    11701 taaagggtta aattatgtgg aaagtattat gaaatttata ggaattaaaa aatttgatga
    11761 attgttagta gatggaactg gaactagtga agaagaaaga caagctgcaa tagaaaaagc
    11821 atctgaaaaa atacatggta taatagatgg tatgaaattt taattataat attttattaa
    11881 aaatataaag gaaaaacctc ttttaatgta gaaacttata ttgaaggagg cgagaatcat
    11941 gcgagaagaa aaaagtaatg aaaagtatga ttgttattgg tgtaatcaag aaaataattt
    12001 ctgtgtagaa ataaaagata atatagtaat gatagatgat ggcactggta cgttaaaaca
    12061 agccgttttc atagggtata aacaaatcca aattaattta aattgttcac attgtcaaaa
    12121 cttaaataga ataaaattaa atttgtagaa ttttaaaatg gaaattattg aaggaattaa
    12181 aaatagaaag tgttaatatt tagactgaaa attatatttt atacaataaa agtttattta
    12241 aataacattg tattttaata tttattttca gtcttacttt attaaaatta ttttttggtt
    12301 atgtctggaa aatatctgtc tactaatttt tctgaaatat tttttaattg gtcagccatt
    12361 gaagtcagac ttaataaggc tatcaataaa tcatctttat tttctggatt tatactaata
    12421 ttttcaaaat tagactctat aaagtccggt atattctttc tttgattttc ttttattaac
    12481 atgaattttt cgtaacatga aactaaatct ttttcactga aattatcctt ttcatacaca
    12541 ccatgtaaga ttcttttaat ttcttgtatt gtaagtgtat ttttcataga ataaataatt
    12601 atcatctgta aaatttgttc tttagtatac ttttttccct taactggttt gattaatccc
    12661 tctttgctgt aattatttat catggtttta gtaagaattt tatcactttc aaatctttta
    12721 ttaggagaaa gtttattgtc aattagagaa ataatttgat ccatatataa gtcaatagag
    12781 ggaatatcgt ttgaagttat atctgcatta tttagtgttt ctagtattat agtattaagt
    12841 tcattattca tcatatgtta cctccataat atatataata cactaaatta gaaagtaaaa
    12901 caatattaag acttgtaaaa aaaacataaa gtggtataat taaatatata atatagtaat
    12961 gaaaactata tttatgaatc tgatttttaa aatacataat tgtaggagga cgaaatgtat
    13021 caattttttt taaagggtag agacccaata agcagtttaa ctcattttat aggtgcttgt
    13081 ttatctcttt tagcaactat aattttagtt tttcaatctg ttacattaca ggaaacttct
    13141 ctcttgatga tagtatctgt atcggtattt ggactttcac taattgcttt atatagtgca
    13201 agttcgtatt atcatttttt aaaggggaca ccagaacaag aattgttttt tagaaaagta
    13261 gaccatgcaa tgatttatgt attgattgct ggctcttata caccaatatg tctgaatttt
    13321 atggaaaaaa aagaaggcat aatatttgtt acagcaatat ggatagttgc atttattggc
    13381 ataataataa aaatattttg gatggatgca cctagatggt tatctaccag tatatattta
    13441 cttatggggt gggcaattgt ttttgatata aatgcattca attcaattcc taaggattgc
    13501 cttagactat taattatgga aggtgtatct tactctatag gtgcaattat ttatataatt
    13561 aaaaagccta atataagtcc tgagtttgga tttcatgaaa tatttcatat ttttattatg
    13621 ataggttctt tatttcactt tttagcagtt ttattgtatg ttttatagtt ttaaaagatt
    13681 tgattaattg atactaaaat tctacaatga ataaaatgga actgccttat atagattata
    13741 tcttcgacaa taagacagtt ccttattata aatattaaat tctacttata ataaaatttt
    13801 gataaaaata gtccattcaa aatgcctatt ccataattcc tttaatgtac ttttcattta
    13861 cgacatatag tttgaattta tgtactataa ttatatcctc taaattctct gaaacactaa
    13921 caattctgac acatttgttt ctaatttcta caggtaaatc taaatcattt gttaaattca
    13981 catagtcatc atatttatac ataaaatcat ccctttacat taagat
//
LOCUS       DAMJVX010000093        14020 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.097, whole genome shotgun sequence.
ACCESSION   DAMJVX010000093 DAMJVX010000000
VERSION     DAMJVX010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14020
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.097"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            108..608
                     /locus_tag="PC407_002377"
     CDS             108..608
                     /locus_tag="PC407_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2497820.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNTINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(858..1637)
                     /locus_tag="PC407_002378"
     CDS             complement(858..1637)
                     /locus_tag="PC407_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497821.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKALDKENNVLGKSKALSFEYKNGNDEISLDTANNVLEKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1634..2113)
                     /locus_tag="PC407_002379"
     CDS             complement(1634..2113)
                     /locus_tag="PC407_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2497822.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRTLLKTKLENTEVII"
     gene            2919..3620
                     /locus_tag="PC407_002380"
     CDS             2919..3620
                     /locus_tag="PC407_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF2497823.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKSALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLLVVFILGHRMRGIVEKMNVKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAVISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIVLS"
     gene            3646..4302
                     /locus_tag="PC407_002381"
     CDS             3646..4302
                     /locus_tag="PC407_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497824.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGLRFGQMPMLSVIFQI
                     WGLVATTYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFITDSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDSNKTKE
                     "
     gene            4509..6149
                     /locus_tag="PC407_002382"
     CDS             4509..6149
                     /locus_tag="PC407_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF2497825.1"
                     /translation="MFDVKIANGIIIDGTGNSRYKGDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPIKLNLHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDKVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWDGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            6508..7830
                     /locus_tag="PC407_002383"
     CDS             6508..7830
                     /locus_tag="PC407_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497826.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMLKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDICCC
                     ITGLEEAYHAMKKENIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPAVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAI
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            8027..9034
                     /locus_tag="PC407_002384"
     CDS             8027..9034
                     /locus_tag="PC407_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF2497827.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDK
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGTMGCPPEILKKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            9072..10469
                     /locus_tag="PC407_002385"
     CDS             9072..10469
                     /locus_tag="PC407_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HDF2497828.1"
                     /translation="MASTNKNNVTSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKINTNKSDINKVNIDTVDTDKLNTNTLTEKNKKVIFKGEDN
                     MSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKITHNIWE
                     EKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIWSNFGA
                     DGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDNLWIVT
                     QLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEMMLEMT
                     SRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYEVERVT
                     CNQREKNEGYDVSIRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKEEWITF
                     DGMDVLKQMYMGVEE"
     gene            10614..11879
                     /locus_tag="PC407_002386"
     CDS             10614..11879
                     /locus_tag="PC407_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2497829.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTDIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            11921..12960
                     /locus_tag="PC407_002387"
                     /pseudo
     CDS             11921..12960
                     /locus_tag="PC407_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
     gene            12995..14017
                     /locus_tag="PC407_002388"
     CDS             12995..14017
                     /locus_tag="PC407_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2497830.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMVAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
BASE COUNT         5033 a         1614 c         2638 g         4735 t
ORIGIN      
        1 tacaaagata ttttaagcta atatatagta atttatatag cattataaac tagaatcata
       61 taaaataagt taactacaat cataaattaa aaaggagaaa tgttaagttg atagaaggaa
      121 taaaatttat aaaggcagaa gaaaagtata tttattcgta ttggcaaact tttgataaaa
      181 tagcaaaaga gaggaaatat ctagctatgg atgaagcctt tccttttgaa gaaacagttg
      241 aatttataaa aaatactata aataaaaact taccacaact gtttataatt gatttagaat
      301 cagataactg tattggttgg tgtgacgttt tacctaaaac tgaaaaagtt ggttatttag
      361 gcatgggtat tcttaaagag tatagggaaa aaggtatcgg aagtagtctt ttaaaacaaa
      421 ttattgattt atcaaaagaa tatgggtatg aaaaaattga gcttgatgta tttaaaagta
      481 atagtagagc tattcatgtt tataaatctt tagggtttgt agaggtaaat acaatatcta
      541 gtggttttac atggaatgat agacctgtaa aagaagaagt aattcaaatg gagcttacac
      601 ttatataaaa taaagttaaa gtctttaaaa ttccatatac acttgtataa aataaagtta
      661 aagcccttaa aattccatat aaaagaaata tttttaaggg ctttttaata atggttcttt
      721 tgaaggtagc taaatatcta aaattttata tattaaattt aaagaaaata taaagatgga
      781 agatacaacc atgtttacta ataaaaaaat taaagtcata aataataaac tcataaacaa
      841 cttattatta cttatgttta ttttgcatct attacttcat gagccatttt aactaagtca
      901 ttcatagata aatttgagtc ttgaactgta aaagaatagt aaactccatc ttgtttccat
      961 gtcaaaaact taaattcatt aacttcaact ttgtctgatt cattatttgg ccctacacta
     1021 aatgtgtatt tgcctgattt ttcatcaatt ttatcctgtt ctgtcatttt ataaccttct
     1081 ggtacaaact tttgtgcgaa tgcttcataa gaaatattaa tatctttgta agtatctaca
     1141 acttttagat tgtcagaact cttctcaagt acattatttg ctgtgtcaag agatatttca
     1201 tcattgccat ttttatattc aaaactaagt gcttttgatt ttcctaaaac attattttct
     1261 ttgtcaagtg cttttttatt tacagtatat gctcctttaa atttatatcc attactaaat
     1321 tcagaaacta tattaggggt aaagttaaag tcttttttta cttgttctgt agttgggata
     1381 gatgtatagg tagccttagt acttgatttt ccaactaaac tatatatatg tcctactgca
     1441 agggctgttg tgctcaatat caaagtaatg gctaaagttg caactaagcc ttttctaaat
     1501 ggtaatttgt gtgttttatt attaatcatt ttataatttt cctctctttc tggtaaacta
     1561 tttagtgttt tacttaaact attatgaaaa ctttctggaa cttctggaaa cgaattattt
     1621 aatatatttt tattcatatg attacctccg tgttttctaa tttagttttt aataatgttc
     1681 ttcctcttga taatctactt tttacagttc cttgaggtat atctaatatt tccttaacct
     1741 catctactga ataaccttca atatagtaaa ggactattgg aattttaatt tttttaggta
     1801 gactttgaat agcagtatac aaatcgctat agtcatcttt ttcatcaatt tttttgtttt
     1861 tgaagacatc ttcaaatgat agagatttta atcgtttgcg tcttaatgaa ttacattcat
     1921 ttatgactat tctaatcagc catgttttaa agtattgttc ttccttaaga gaatccaact
     1981 tttcatatgc ttttaaaata gcattgttga cggcatcttc acagtcttgg tcgtttccta
     2041 gaatagactt agatactcga tacaaagtct gttctgattc aagtatattg tttgtaaatg
     2101 tagttttatc caactgagtt ccttcccttc tatgcaaatc gtagcgctac ttttttgcct
     2161 ttcacttatt agataaaatt ttactataga tggttcactt tttttatgat tatttgataa
     2221 atttaagttt gaaagttttg cctattctat acaaatattc tataatatat ttttttctag
     2281 tattttcccc tcatatataa agccatgtag atatattaaa ctttagaagc catttatata
     2341 tttagtttta tagtaagtgt ttatattaag taattataaa atagtaagta tttgatgttg
     2401 agtagttaga aaataatgct tttggattat ttacttcatg tatagattta aaataagttt
     2461 tttaattaat gactaaagat aacggtattt tgactaaaag taggctttta agaaaaaaca
     2521 atgaaactta aatgagaatc aagagttaga aatgtcatta ttttagatag atatgaacat
     2581 aaataaactt aaaaaatata aattataaaa ataataattt ttttaaaata acaaagtttt
     2641 ttgattaaaa gttttatatt gaaatgtcaa agtaataaaa taccttataa gttaattaca
     2701 ggttaaaaaa ataatatgtg aattttctga tatttactaa ttatcaataa agtatttaag
     2761 tattaatgtt tttagtgaga ctttaagaca gataatctcc tattaaagat actatatctt
     2821 agataaaata aattaaaaca atataaaaac ataacaagat tacagaaaga aagctcataa
     2881 taaaacacta aaaaatttat tttaaggagg aataaagtat gaagtttcaa gaaatgatta
     2941 atagcccagg tctttggatt gtttctagtt ttttagtaat tatttcagtt gttcaagcaa
     3001 tcgtatttat gaaatcagca cttaaagagg cttcaaatct tggtattgaa agaaaaggaa
     3061 ttaaagcagc aattcgctca gctagtgtta cagcaattgg accctctcta tctcctgtta
     3121 ttacattatt atcactggta gctgtgattg gtgctcctac gacttggatg aggctttgtg
     3181 atgtaggagc tgccagaaca gaacttggag taatatctct tacatcaaat ttatctggag
     3241 tagaagtagg tagtgctgca tttggagcag aagctttttc gtatgcacta tggggaatgg
     3301 ctttaaataa cttaggttgg ttgcttgttg tatttatttt gggtcataga atgcgtggaa
     3361 ttgttgaaaa aatgaatgtg aaatataatc ctacttgggt taaaaaacta cttgcagggg
     3421 ctacagttgg attatttgct taccttcttt caaatcaaat aaaaacattc gaaataccaa
     3481 aacttacacc agcagttatt tcagctatag ttatgctagt attgacgact ttatttaaaa
     3541 agaatcaacg attacaagaa ttatccttag gtattgccat gttgattggt atgtttggca
     3601 cacagattgt actatcatga ttaaaacaga taagaggtga taattatgta tgataaaaag
     3661 atgataaaaa tagggagaat ttctataggt cttgcaatta ttgcaaattt tattcctgca
     3721 atctatgtag gtcttcgctt tggtcagatg ccaatgcttt ctgttatatt ccaaatttgg
     3781 ggattagttg ctacaactta tggagtgagt tggattgttc aacctatagc ttattatcca
     3841 acactaggag ctccaggttc ttatataggt tggcttgcag gttctgttgg agatatacgt
     3901 atgcctgcag catcaatggc acagaaaatt gcaggtgtag aagcaggaac tcatgagggt
     3961 gaagttgtag gaacaattgg tacatgttgt tcaatctttg tatcagcttc tatgattact
     4021 atttttacca ttgtaggcta taaagtaatt cctttgttac ctgaatttat tactgattca
     4081 tttaattata tacttccagc attgtttggg gcaatttacg ttgatattgc gcgtaaagat
     4141 attcgagctg gtggatgtac gattgctgta gcattagcta ttatgtactt tggtgaaaaa
     4201 attggtatac caggtggatt gttgacattg tttattgtgt taggtgggat acttattaca
     4261 agggttttct ttgtaattga tagcaataaa acgaaagaat aggattgttt actaggaaga
     4321 aagactagaa ttatttgctg agaaaaagaa taggattgtt tactaggagg aaagactaga
     4381 attatttgct gagaaaaaga ataggattgt ttactaggag gaaagactag aattatttgt
     4441 tgagaaaaaa gaataggatt gtttattaag aaatatgaat ataaataagt cttgaaagga
     4501 gaagaagaat gtttgatgtt aaaattgcca atggaataat cattgatggt acaggaaatt
     4561 caagatataa aggtgatgta gggattgtag gagacaaaat catggctatt ggagatctaa
     4621 gtcaaaaaga ggctaaagag actatagacg caactggaaa atttgtatca cctggtttta
     4681 tagatttcca cactcattct gatttatctc ttgtgtatga caaatacaca agaagccgta
     4741 tccatacagg tgtaacaaca gacgttattg caaactgtgg tataggagtt gctccgataa
     4801 gagaagagaa gaagcaggag ttaattgact atcttggaac gaggataatt ggtacaattc
     4861 caataaaatt gaaccttcac tggaatacaa tgcaggaata ctttgattac ttaactgaaa
     4921 attctccagc agtaaatgtt gtagcttatg tagcacaagg acctgtacgt atcaatgaaa
     4981 tgggattttc aaaggagcca gctactttag agcaacttaa aaatatgaag ttggaagtta
     5041 gaaaggctat ggaagctgga tgtgtagctc tttcgtcagg attagtttat ttaccaggag
     5101 catatacaaa gaaagaagaa atggctgaat tatgtaagga attgatacca tacaatggat
     5161 actatatatc tcatattaga gatgagggtg atgaagaaat ggaagctctt gatgaagcaa
     5221 tttatatagc taagacggca ggagttcctc tacatatttc tcatttaaaa gtcatgggtc
     5281 ataagaattt tggaactatt gataaggtat ttaaaaagct agatgaagct gaggcagatg
     5341 gcttggaagt tacttttgat tgttacccat atacagctgg aatgacatct ttgggtgcac
     5401 ttttacctcc ttgggctttt gaaggtggtg tagaaaacat ggtaaaacga ttagaagtgc
     5461 aagaaaatag ggacagaatt ataaaagagc tagaggaagg gattcctgga tggcaatgtt
     5521 tctaccagtt agcaggtggt tgggacggtg ttgtactagc ctctgtaatg actgaagcaa
     5581 ataaatatgt agagggtaaa actttaatgg aggtagctaa aatcaatggt gaaaatccat
     5641 ttgatacgtt ctttaggctt ttgattgaag aaaaaagtaa aattcaagta gttgttcata
     5701 cacaaggtca agaggataca gataaggtcg tttgccatcc aaagtcatgt ataggttctg
     5761 atagtatgga cctttctaca gaaggcttac tttctcttgg aaagcctcat ccccgtgcat
     5821 ttggtacttt tggacgaatt ttctcttact atgttagaga aaaaggtatg ttgacttttg
     5881 aggaagcagt taaaaagata acttatcttt cagcaaaacg cttaggaatt tataaagaaa
     5941 ggggtctact taaggaaaat tattttgctg acattgtggt atttgaccca gatacaatag
     6001 aagataaagc gacttatagt aatcctaaac agtatacagt gggagtggaa tatgtattgg
     6061 taaatggtaa aattgcattg gcaggaggaa agcaaactga tgtatgtgct ggacgtgtaa
     6121 ttaaaaatcc tcttagcata gcaaaataga atacattaag aacttttata aaaatagtct
     6181 ttctttgaat attaaggaag gctatttttg ttaaattaat aaagattact ctacttgtgt
     6241 tttctagaga atttaacttt aagtagaaaa aacaatatat taattgtaaa atgtaattaa
     6301 tattaatgga taagatttaa atacaaaaac ttaaatacaa aaatgtttta acagatagtc
     6361 ataattaaaa aacttaaagt aactttttat ataagatata attgattatg caaaatttta
     6421 tgaagcagca accttgttac tttaagtcaa gggaggttca gaaagtgtct taagagataa
     6481 tagtatcttt aggaaaggag aaacgaaatg aaaatacttt atgtaggacc aggcatggta
     6541 atcgacaaaa tactagcagt aagtaaaaag aattttccca atattgaagc agattttctt
     6601 agatatgata gatatattga agtacctgat ttattggaaa aatatagagg tagagtagat
     6661 gcagttcttt ttacaggtaa atcgccattt aaattttttg aaaaaagaca aaaagatttt
     6721 gtgttgtctg attatattcc tagacatgaa acaacattat acagagtatt gcttgaaatt
     6781 acatatttgc ttaaattcga tattagtaga cttagtatag atacttatga taaagctatg
     6841 ctgaagagat tgtataaaga aattggagtt gaatttaagg atgaacatat gttttttgca
     6901 gagcaaaagt atttagaaga ggactacata aagtttgtgc ttgaatttca taaatataat
     6961 tatcaaaata atgacatatg ttgttgtatt acaggtcttg aagaagcata tcatgcaatg
     7021 aaaaaagaga atattccagt agttttagcc ttgccttctg aggatgtaat ggtacaaagt
     7081 ataaaaaaca ttcagatgcg ttatattgca aagaaaaaca atgagaatca gattgttgtt
     7141 cttgctatca aacttaatat gccttcagaa tattcactga tgaaggaaga tgagtacgct
     7201 tatctttcac aaaggattaa gattcttgat aaactatacc atttcaatag ccgtatagat
     7261 ggtgttttgg tagagcagag tagaagcgaa tttatgattt ttacaactaa aaaaatactt
     7321 gagttagaaa caaagaagta cagagatatt tatttgttgg atatgattcg tgaagtttca
     7381 gttataaaca cttatatagg aattggttat ggaaaaacag caaatgaagc taaatttaac
     7441 gcttatgaga gtattaagat ggctcaaagt caaaaaaatg atgcagcata tgtagttttt
     7501 gaaaatggag aaattatggg accactaaag ccaaatcctg ctgtcaaaga gaaaaatagc
     7561 tttgatgaac gattttatca aattgctaca gagacagggc taagtgctaa tactgtatat
     7621 aaagtgttca ataaaataat aaatagtgga aaaactgaat ttacatcaaa ggaattagct
     7681 attatatgtg gtgtaagtat tagaactatg gacaggatta tacttagatt gtgtgattct
     7741 ggttattgtg aggtagtgag tgaacagctt atgagcaagt atggtagacc cagtagaata
     7801 ttacgtttta aaccttttct gttgttttaa atagatataa gttaaaaatt aaatttttat
     7861 gataacattt tctttacaat tttttagata tatgttaata tttagaaaaa tgcatatgta
     7921 ttcataaaat aaactcaaaa tagctaaaaa atcattaaaa aggaaatcgt atgcataaaa
     7981 tgagaaagtt aatttataac ttaaataaat ctgggaggag ataattatgg gaaaatatca
     8041 ggattcaaaa aaagttgtaa gaaattattt tgaagcatta gaaaatgcta ctggggatga
     8101 ggttgaaaat gtcttaaagc aacatatgaa atcagattat aactggaaag gtgtgtatcc
     8161 atttcgtgaa caagaaggca ctgaaaatgt agcagatgta ttttggaagc ctttaaaaaa
     8221 atcattgagt aatatgcaaa gacgacaaga tattttcatt gcaggaacaa atgagattga
     8281 caatagtgaa tgggtaatga gtatggggca ttttatggga ctttttgata aggattggct
     8341 tggcattaaa catactcgta aaatgattag tttaagatat gcagaattta actgtgttca
     8401 agatggtaaa attgctaaaa ctggattatt tgtagatata attgggttta tgattcaagc
     8461 aggagttaat ccattgccac cacaaacagg ttcatatttt gtatatccag ggccaattga
     8521 ccataatgga ttactttttg aagatgcaga tgaagttgaa ggagtaaaaa ctttagcatt
     8581 agtaaataaa atggtagatg atttatctga attaaatgaa agtggtacta tgggatgccc
     8641 accagaaata cttaaaaaat cttgggctaa agatatgatt tggtatggtc caggtggaat
     8701 tggtgcttct tatactatac ctagatatca agagcaacac cagctaccat ttagaaataa
     8761 tttaaaagga aagactttca atgggcatgt atgtagattt gcagaaggta attttgcttg
     8821 tttctttgga tggcctaatc tgactaatgt accatgtgga ggttttttag gattaccagg
     8881 tggagaggtt aaagcagata tgcaagttgt tgatgtttat tatcgtaagg gcgataaact
     8941 tcaagaaaac tgggtgttaa ttgatattcc ttattggttg aaacaacaag ggcttgatat
     9001 tcttgagaga actaaaagta tttttaatgc ttagttatgc atacgtattc taaaacaaag
     9061 ggggatataa agtggcatct accaacaaaa ataatgtaac aagtaaagag gaagaaaaag
     9121 aagtttctac aagtgggctt gaatcatgta cggttaaaga aaacaaagct aaaatgaatc
     9181 aaactaattt tagtaatata aatgaaaatc aaacaaatga gaataaaacg aacacatata
     9241 aaggaaatat agataaaata aacacaaata aatccgacat aaataaagtt aacatagata
     9301 cagtggatac agataaatta aatacaaata ctcttactga aaaaaataaa aaagttatat
     9361 ttaaggggga agataatatg tcatctacaa aaatttacta tggagattca ttagaagtaa
     9421 cgcctgttgg agtaatggat tacaatgatt tttcaaaaca gacaaaaagg gaacaggaaa
     9481 tacctggatt tgattctaag tatagagatt ttgtagatta tattatgaag attacacata
     9541 atatctggga agaaaaaggt ataggtgtta tttatgatac atatcacaat aatgttacaa
     9601 tgcattgtgg ctcttcaaat ttagttggta ttaaagatgt tatttccaat actcttcaaa
     9661 cattacatgc gtttcctgat agaagattaa ttggtcaaaa tgtaatatgg tctaactttg
     9721 gtgctgatgg ttttttatct tctcaccgtg ttttgtctac agcaactaac ttaggtgata
     9781 gtaattttgg accagcaaca ggtagaaaaa taaactttag aactgtaatt gattgtgcca
     9841 caactaacaa tagaatccac gaagaatggt tggttagaga taatttatgg attgttactc
     9901 aattaggatt aaaccctcag gaagtagcaa agagtatggc aaaggcaagt gaatctaaag
     9961 tgcttagctt acaatcaact tatggtatct gtgaatctat ggatggtcaa tttatgccaa
    10021 ctaagtatca agcaaaagat gattctgttg gcgaaatgat gcttgaaatg acaagccgta
    10081 tttataatta taaatatata aatgaagtta aaaaatacta tcatgataat gcagtagtac
    10141 attttatatg tgataaagat ttaaatggtt atgatgagat tcaaggaatg attgtaagtt
    10201 tacttgcatc aattccaaat ggaagttatg aagtggaaag agtaacttgt aatcaaagag
    10261 aaaaaaatga aggttatgat gtatctataa gatggagatt acgtggaata aatgaaggta
    10321 taggattttt tggacagcct tctggaaagc atatagaagt aatgggaatc aatcattatc
    10381 atatacttca aggtaaagta aaggaagagt ggataacttt tgatggtatg gatgtattaa
    10441 aacaaatgta tatgggagta gaagaataaa gctgtttaga gtataaataa ttttgaaatg
    10501 tatataggga agtattgctt tataaaattt aagtggggaa tgtatatgaa aaatagatgt
    10561 ttaaactttt agcaaaaatt atgactaaat ttaaaagggg gaattataaa aatatgaaaa
    10621 caacggttga aatagaaaat gatatcatat taggaaaaga ctattcaaac ctaaaaagag
    10681 gaattgcatt tttttcaatt gctttaatat acttttttta ttgctacaat tttatggtgg
    10741 gtacttttat taaaccaaca atgatttatg ctttagcaga tgggggattt ggcttttcat
    10801 taaaacaaac agaagaaata tttgcagtta tgtcttttgg tacaattcca ggtacattta
    10861 tttttggggt gatttctaca aaaattggga agaagaggac tttaattagc gttgctttac
    10921 tgattggatt gacaacattt ataccaatgt tatctcctac agatattatg ttatggaaaa
    10981 tagcacgttt gtgtacagga gtagtgttgg gaggcgtatt tggaacagct atgcctttag
    11041 ttgcagatat gtttccaagt aaatatcgtg gtaagttagc tgctatatta actgctttat
    11101 tttcattagc aatgattttt ggtgggaaag tatatggcgt tttaggtgat gcaaattggc
    11161 aaatattgat gtatactgcg attataccac ctatagttgg agctatctta gcaatcttct
    11221 ttgtgccaga tgatttggag tatacaaagg aattagttaa aaagggaaaa gaaactggtg
    11281 aaaagattag ctatataagt atgtataagg gaaaatactt atggattgga ttgggaacga
    11341 ttcttttatc aggtgcaaat tttacagcat attcagcatt ttcaaataat gcaactacat
    11401 acctagtgac aggaatagga atgtcagcag cagtggctgg gtctatatat agtttacaag
    11461 gaattggtca attagtagga tatttattct ggggttcaat tgcagataaa tttggaagaa
    11521 aaataccttt tataggtatg gcattagcag cagtatttgt ttttatatat acaaaattag
    11581 gtggagataa tacaacaaca ttctatatga tatcagtatt actaggtgtt gctgttggat
    11641 attctggagc atggggagca tactacacag agttgtttcc aagtaaatat cgttcgttgt
    11701 catctggtat gtcatttaat ggaggaagga ttatatctac gtttgcaata cctgctatag
    11761 ctggtacagc ttctggtact ttaggtatga tgactatctt ttacatatca atcgcagtct
    11821 ttgtagcagg tgctattgta tggttattct taccagagac gttaaataat aaaaaataaa
    11881 atactaggaa tttattttgt tacttagaaa ggggtggctt atgatttcat caattgatgt
    11941 atgtattata ataggatatt tgctattaat gttagttatt ggttattatt caggaaaaga
    12001 taataaaaat caagaagatt atttcttggc aggtaggtca atgccatgga ttccaattgc
    12061 tttatcagtt gcagctacta tgattagtgc aaatggcttt ataggtggtc ctggatgggc
    12121 atatgtagat ggaatgtatc cagtaatgta aaatgtgcaa aaaattcttt tgtagttagt
    12181 gcatttgtta tgaatttagt ttactacttt atgctattgg taggagtaat tttatttgtt
    12241 ttttatgggg gaaggacatt tgaaacttca aatgaaataa tgattacttt tattttaaat
    12301 gaacttccag taggagccat tggaattatt attgcaggtg tgtttgcagc agcaatgtct
    12361 agtatcgact ctatattaaa cagtatgaca actgttttta cgaaggatat ttatgagttt
    12421 tatttcaaaa aagacaacaa agaatcatca ttaaaagtta caatgattat aactgtagtt
    12481 ataggagttg ttatgacagg ttttattatt atgggatttg gaggaagcat caaatcaatt
    12541 cttgaccttg ttggtaacta tatctcatac tttgctggac cagctacagg tgcatttgta
    12601 ctagcaatgt ttacatataa agctaatgat aaaggagtat ctattggttt tattgtaggg
    12661 ttagttttag gattttttat ttctaataaa tataatgtga gttggttgtg gaatccagca
    12721 ataggtgcta caataacatt gatatttgga tatattttta gtgtcatttt aaagaaaaat
    12781 cataaagtag atgatattaa aaaatacact gcatttggta tgagaaatca ccttatttct
    12841 caaggaatgg ataaagtaga tggagtatct atagtgccat tttctttagg taaacaagaa
    12901 ctaattgtat tagctttttt tattatccaa tatattatat tatttttgat acaattttaa
    12961 gaagaaatac tagatattta aggagaaata ctaaatgaat caaaagaaag ttcattataa
    13021 taaatcggct acatcaaaag atgtcgcaag attggcagga gtatctcaat catctgtttc
    13081 aagagcattt ggtagtgcaa atggaaaggg cgtaaaacct gaagtaagag aaagaatttt
    13141 tcgtgtagca aacgaaatag ggtatgttcc taatttggta gcacggggaa tgattagtgg
    13201 aaaaactaat gttataggtt tgatagttgg tgatagtcta ggtccatttt acaatagaat
    13261 tatcaattta tttgtagaaa aaattcaaga gataggtaaa cagtgtctcg tttttaaagt
    13321 tcctagacaa caaagtatag atagtataat ttcaaaagta attcagtttc aagtagaagc
    13381 tgtgattatt acagcttcgg ccatgaataa ggtgatggct gagacttgtg aagaaaacaa
    13441 tattcctgtg attcttttta atcgttttat acctggtata aaaataagta cagtctatgt
    13501 agacccaatt gaaggtgggg gcatggttgc tgaatatctg cttaagaaag gtcataagaa
    13561 tataggatat attcaattta cgagagaaac aagtgaagaa atggaaaaga aaattggatt
    13621 ttattctaag ttaagacaaa gtgggattca taaccttaaa gaagaatctg caaactatga
    13681 ttatgattct ggatatgaag caggtaagag aatgttagca ttatcaaatc ctccaacagc
    13741 aattttttgc acaagtgact tgatagctat tggtgtaatg gatgctgcaa ggtttgagta
    13801 caagttgagg gtgccagagg atttatcagt gataggatat gatgatattc aaatggcttc
    13861 atggaagagt tataatttaa cgacgattag acaaccacta gatttgttga ttgaaaaaac
    13921 aattaacata cttaagtcct tattaaatga agaaaatcca gagtcagtcg ttgaaatgtt
    13981 aaaacctgaa ttgattgaaa gaggttctac actttaagtg
//
LOCUS       DAMJVX010000094        13741 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.098, whole genome shotgun sequence.
ACCESSION   DAMJVX010000094 DAMJVX010000000
VERSION     DAMJVX010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13741
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.098"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            564..1994
                     /locus_tag="PC407_002389"
     CDS             564..1994
                     /locus_tag="PC407_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2497831.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            2097..3200
                     /locus_tag="PC407_002390"
     CDS             2097..3200
                     /locus_tag="PC407_002390"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2497832.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMTYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            3215..3451
                     /locus_tag="PC407_002391"
     CDS             3215..3451
                     /locus_tag="PC407_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2497833.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEKACQEA"
     gene            3468..4517
                     /gene="vorB"
                     /locus_tag="PC407_002392"
     CDS             3468..4517
                     /gene="vorB"
                     /locus_tag="PC407_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2497834.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4519..5259
                     /locus_tag="PC407_002393"
     CDS             4519..5259
                     /locus_tag="PC407_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2497835.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            5262..5801
                     /locus_tag="PC407_002394"
     CDS             5262..5801
                     /locus_tag="PC407_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2497836.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLLEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5925..8024
                     /locus_tag="PC407_002395"
     CDS             5925..8024
                     /locus_tag="PC407_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HDF2497837.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKMIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWALDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNTKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(8291..8806)
                     /locus_tag="PC407_002396"
     CDS             complement(8291..8806)
                     /locus_tag="PC407_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HDF2497838.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8898..10109)
                     /locus_tag="PC407_002397"
     CDS             complement(8898..10109)
                     /locus_tag="PC407_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2497839.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDFPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            10785..12227
                     /locus_tag="PC407_002398"
     CDS             10785..12227
                     /locus_tag="PC407_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2497840.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            12269..12622
                     /locus_tag="PC407_002399"
     CDS             12269..12622
                     /locus_tag="PC407_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497841.1"
                     /translation="MKKSNIFSIIFPTIIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            12813..13634
                     /locus_tag="PC407_002400"
     CDS             12813..13634
                     /locus_tag="PC407_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2497842.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIKKVKSNNIA"
BASE COUNT         5253 a         1598 c         2451 g         4439 t
ORIGIN      
        1 ttttagatta atatagctat aataatttat gatattagat ggaaattacg taaaaataga
       61 aaattgaaaa ttctgaaatt tttgcatatt gatatttatc tcatagattc ttttttgtaa
      121 ttgtaacacg cttttatttt taaatcaata gtacatgtat aaaaataaaa ggaatctaaa
      181 taagaaaaag atatttttaa tatctggatg tagtatagaa atatcttgaa taaattattc
      241 ttgaataaaa ttataaaaat ttaaaatcac tagaaatatg ataaaataat ttaaaatacg
      301 catttcaatg acatttcaag acaagaaaat aataaaaaac aaatctaaat taagcctaaa
      361 tagcttttta gttaatttta gtagtaaaaa ataaaactta aaaagtgttt gacaattatg
      421 aattttaaga atataattaa attaaaatta tacacatata aaaatatact cctgaatcaa
      481 gaatgagata tttaagattc aaaatatata ttattatacg tgaataatga aaagtaagaa
      541 aattttataa gggaggggta gatgtgagta gtcaagaaag caaaccaaaa ggaaatctta
      601 tatcaaattt tagagatgat atgagagacc ctaagaaaag agcaatatac acatattcaa
      661 gttctataaa cttagcgtac aattttatag gttactttgt atcaactgtt acaaatattt
      721 ttttaactga tgtggcaaaa cttggtgtaa ttatggcagg tggtatgcaa attatacaat
      781 ctttcataaa aattgctgta gctcctataa caggtacagt atttgacaaa caaccattca
      841 aaaaaggaaa gtattatcca tggcttagat atgcaccagc agcgttagca ttaacatatg
      901 gaatattttt catagttcca ttaatgaaaa taagtccagc attactttta cctttgatga
      961 cagctttatt attaatagca tcttgtgctc agcaagtagt tttcactata atttatgcta
     1021 tttaccctgt agttgctaag acacctagag accgagttat aggctcaact actactaata
     1081 tatttaaaga agttggtaaa ttcttagtag gtttttcata tccgttatta ttagttttat
     1141 ttgcagatgt attaggtgga gaaggaatgg gttatttagg tacctactta ttctttgcag
     1201 caggatgcct tttagtattt tggttttctt caacagagat aataaaatct ggagcagaag
     1261 aagaagttct tcaaaaagaa gagaagccaa agattaaagc aagtgaaatg gcaaaagctc
     1321 tattcactaa tgtaccactt atgatggcat tttgtttaga atttcttata tgtataagat
     1381 ctataggatt aggacctttg gctccatatt attttaaata tgtaatagaa gatgaacgtg
     1441 gattagcagt atttttatca atcatgcctt tggtaagtgt agcattcatg tttttcgcac
     1501 cagtgtttat aaaaatctgt agagagcaaa aactagcttc aataatttct ttttctatat
     1561 gtgcactatg ccacttattg gttgcatgga ctccttgggg gaaaactact ttaggagtaa
     1621 ctatgttgct ggcaattggt ggaggttttt caaatgtagt atcaataata aatcttaatt
     1681 tctttgccgg ttcttgtgat tatgggcatt ggaaatcagg aaaagactta cctggcttaa
     1741 gtatgtcact atatccagtt gcaatacaag taggagtatt acttgcaact actataagaa
     1801 cagttttaat gaactcaatg ggatatcaag ctgatatggt agtcacagaa gcagttaaat
     1861 caggttttat aaatatgatt tcttattcta tggcaatacc tttaataata gctgttgtta
     1921 tagcaatact atatccagta agtgacaaga aattaaacca aataagagaa gagttaaatc
     1981 aaagaaatgc ttagttaaaa gaaagttaag tagatggtta atttaagcta ttaaagtaag
     2041 tagacgcaat agcgtttata taaatcaaaa tacatataca gagggggctt ttaaaaatga
     2101 tagaaaaatt attgagaaat accgtaaaag aattacatca atatgttcca ggtgaaccaa
     2161 tagaaaaagt taaggaaaaa tatggtgtaa aagaaataat aaaattagct tcaaatgaaa
     2221 atcctctagg accatcacca aaagcaatag aagctatgat tgaaatgcta aaacaaggtc
     2281 aactttatcc agagccagaa gctaacgaac ttagaagaaa attagctgaa aagctagatt
     2341 taaaaccaga aaattttatt gttgctaatg gtgcggataa cgttatcact ctaataggtg
     2401 aggcttttat aaatagagga gatgaagtta tatattgtaa cccaacattt ccatcatata
     2461 gaactgcaac tataaaaaat gaagggatac cagtagaagt gcctttaaca gaagattata
     2521 agtatgattt acaaggtata ttagataaga ttacagataa aacaaaatta atatgtgtat
     2581 gtaaccctaa taatccaaca ggaacaattg tggatgataa agagttagaa gaatttttaa
     2641 agaaagtccc tgaaaatatt ataactatat tggatgaagc atatatagaa tttttaacag
     2701 ttcctaatta tgtggatgga cttaagtatg taagggaaaa ttataacgtt atagtaacaa
     2761 gaactttctc taaaatatat ggtcttgcag ggcttagagt aggatatggt attgctaaag
     2821 atgagataat aagaacttta tttacagtaa aagagccttt ctctgcaaat cgtgttgcta
     2881 taagtggagc aacagcagca cttgatgatg aagagtttat aaaagaaaca tatgaattaa
     2941 atagagttgg aatgacttac tttaaagaag agtttacaaa aatggggttt gatgttgtag
     3001 attcacaatc aaatttcctt tatgtagata tgaaaacaga tatacctaag ttatttgaag
     3061 acttgaaaaa aagagggttt gtaataagac caagtgctac acatgcacgt gtaagtattg
     3121 gaactatgga agaaaataag aaatttgtag cagttttaaa ggaaatatta aatattaaag
     3181 gagagccaag tattggataa tagatagaaa tattatgaaa cggggtgtag atatgaactt
     3241 aaaaataaac gcagagagat gtaaaagttg tgaatactgc gttatatctt gtaaaaaagg
     3301 agcattaaaa atatcaagta agataaataa agaaggttat gctcatgtag aagttgatga
     3361 gagtaaatgc atactttgtg gaatttgcta tcaggtgtgt ccagataatg tttttgaaat
     3421 aattaaagaa aaagcatgtc aagaggctta gaaggagttg ggataatatg aaaaagatgt
     3481 ggaaggggaa ccacgccatt gcagaagcag ccctaagagg aggttgtgaa ttttatgcag
     3541 gatatccaat tacaccacaa acagaggtaa tggaattttt atcacataga atgtctgaac
     3601 ttggaagaac ttttatacag tccgaaaatg agatggctgc tatatatatg gtatatggag
     3661 catatgcaag tggaatgagg tctatgacga gttcttcagg accaggaata tctttaaaac
     3721 aagaagggat ttcttactta tgtgcaaatc attacccttg tgtaatcgta aatgtacaga
     3781 gatgggggcc tggtcttggc tcattggact cagcacagac agactatcta agagatacta
     3841 gagggggagg aaatggagat tatcatttga tagtttatgc accaaacagt attcaagaga
     3901 ctgtagattt gatgtataac tcatttgatg tagcggaaaa atacagagtg cctgtcgaaa
     3961 ttttgacaga agcagcttta ggtcaaatga tggaaccagt agaatttcca gaatttaaga
     4021 agagagaagg agatttaggt tggacttatg atggaagtaa tagagaccat gcaaaggttg
     4081 cagataacca aaagccaact ttttgtatgg agaaacgtat gagaataact gaaaatgaac
     4141 aacaatggga agattatcaa gtagaagatg cagaatatgt gtttgtagcg ttcggtatac
     4201 catcaagaac aactatgaat gctgttagaa gacttagaga acaaggtgaa aaagttggaa
     4261 taataagacc aataacagta tggcctttcc catataaagc atttgaaaag gtaagtaaag
     4321 atgtaaaagg atttatctca gtagaatcaa cagacacagg tcaattagta gaagatgttg
     4381 ctttagcctc aaagaaagta tgtaaagaaa atgtacctgt gtatggttta tttagtggaa
     4441 atcatatacc aaaaacattg caagtaatgg acacttatag caaaataaaa tctggcgaaa
     4501 tcaaggaggt gttctaatat ggctacagaa aaattaacac ctagcgttgt ttcaatacca
     4561 aacaagtttt gcccaggttg tggtcatgga atagtaaatc gtataatagc agaggtaata
     4621 gaagaacatg gatatgaaaa aaatcatgta ttaacattag gagttggttg tgtctgcaat
     4681 atgaatttta gctggaatgg tgataagatg caaactgcac atggtagagc ttcatctaca
     4741 gctataggag ttaaagtagc acttccagat acattagtaa tgacttacca aggtgatgga
     4801 gatgcttatg ttataggtct ttctgaaact ttaaatacag catatagaaa tcataatgtg
     4861 actgtatttg taataaacaa taataatttt gcaatgactg gtggacaaat gtcatggacc
     4921 actatgccgg gtcaagtcac aacaacaagt gtaaatggaa gagatataaa gactactggt
     4981 agtccactga aagttcctga gatggtggca aacttctttg atgtagcata tgtagcaaga
     5041 ggttcagttc atagcccaaa ggaaataatt aatctaaaaa aatatataaa aaatgcaata
     5101 gaagctcaat taaatggaga gggatattcc ttagttgaaa tattagcacc ttgcccaact
     5161 aactggggtg tatcactaga gaaatctatc aaatggatgg aagaagaaat agtgccatat
     5221 tatgctcttg gcgaatttaa gcaaagggat ggtgaatagt tatggaaaaa gagctagtat
     5281 ttgcagggtt tggtggtcaa ggagttttaa ctcttggtct gattgtagct gagtcagcac
     5341 ttgaattggg gaaacaagta acttggatgc ctgcatatgg tccaactatg agaggtggaa
     5401 aagcctatag tgtagtaaag ttttctgatg agagtatagg tgggcctgat atggaagaaa
     5461 tagatgtttt agtagcaatg aataaaccat ctttagatta tataaattta gttaaagaag
     5521 gtggaacagt agttattaat acatctgcaa tagatgagaa tgtaacatta agagaagata
     5581 taagtgtagt taaaataaat tgtcaggaat tagctcaaaa agtaaacaat ccaaaagcag
     5641 caaatatagt agtacttgga gcattaattg ctaaaactgg gttacttgaa aaagaattag
     5701 ctttaaaaac tatgtgtgat ttctttgagg aaaaaggaaa aggaaagttt aatgttcaaa
     5761 atgaagcagc attcatggaa ggctataata acgccatttg ataaattacc cttatcttaa
     5821 gtgatttatc tcaagattta ataatcttta gtgccaagag aaacttcatc ttgagataaa
     5881 attcacttaa aatcacaact ataaacatat aggagcgtga atttatgagt ttggaaaaac
     5941 ttattagacc aaaaagtatt gctatagtag gagtgactga taaacttggt tttggaagaa
     6001 gtgcagcact tagtatagta aaaagtaagg aaacagatag agtatattat gttaatccca
     6061 aaagagaaga attgtttggg agaaaatgtt ataagatgat acaagaagtt ccagaagtag
     6121 tagactgtgt agtagtatgt acgcctagaa atgtagttcc atctgtatta aaagattctg
     6181 gagagttagg aacaaaggca gcagtggtat atgctagtgg atttgcagag gaaggtacag
     6241 aggaaggtac agatttagaa aatcaactta tagaaatatc aaatacatac gatatgaaaa
     6301 tattgggtcc aaactgcatg gggttgctaa attgtataga taaggttaat ctatgggcag
     6361 gaggttctaa atgggcttta gatactaaaa aacctggtat aggaattgta ggtcaaagtg
     6421 gatttataac tgctgaaatt gtttcaagtg attattttaa tatatcttat ggtttttcaa
     6481 caggaaatgg aaatatagtt acactagaag atgcagtaga tttccttgta gatgatgatt
     6541 atgcatcagt aatagcaatt taccttgaag gtttaaaaaa tcctcaaaaa tttatagatg
     6601 ctttaaaaag ggcagcacaa aaaagaaagc cagtaataat attgaaatca ggaaggtcag
     6661 aaaaaggtgc tatttctgct gcctctcata caggaaattt agctggttcg agtaaagctt
     6721 ttgaaagtat ttttgaaaaa tatggagtaa tttctgtgga aaatttagaa cagtttatgt
     6781 gtttagcaca agcttttagt gttttagatg gtaatttacc tgcaaattct aattttgcag
     6841 caataaactt ctcaggagga gaaaatacta tttgtgcaga tttagcagaa gaaaatggtg
     6901 tagaacttgc agaaatatct tctgaaacta aagaagaaat gaaaaaatat cttccaggat
     6961 ttgcaacacc taaaaatcca ctagatgcta caacagcatt attccatgaa aaggatatga
     7021 tagtaggttt attacatacc tttgaaaagg atgatagtgt tggatttact atggtaggtg
     7081 caaatataag agatgaagaa aatgaaatgc atgaaaccct ttgtaaagct gtaagtgaag
     7141 ctagagaaca aggattgaaa aaacctgtgt ttgcagttcc tacacttgaa ggaaatagat
     7201 atttagatta taggactaga ttagaagata atcaagtgcc tataatgtca tcagttggaa
     7261 ctacatttac ttgttttaat aaaatggcta agtttataga ttatgattat tcaaaaagaa
     7321 ctttagaatt taaagctgtt aagaaaagag agtcaaataa tgtagtagca ttatcagaat
     7381 tagatagtaa gattgagatg aaaaaatatg gagtaccagt tccaggacaa ggtaatacaa
     7441 aaagtattga tgaattagat gaaatcttaa aatatattaa gtatcctgtt gttttaaaaa
     7501 taaattcttc tgaaatattg cacaaatctg atgttggagg agttaaaata ggaattaaga
     7561 atagagatga ggcagtagat gcatataatg agattttaac taatgtaaaa aaagcaaaac
     7621 cagatgctaa tattgatggt atattagttc aagaaatggt agaaagtggt atagaaatta
     7681 ttattggtat aactaatgat gaccaatttg gaccaatgtt attagtcggt ttaggaggag
     7741 tatttgtaga ggtatttaaa gatactacat tatatccatt acctataaat catgatgaag
     7801 ctattatgat gcttaaaaaa ctaaaatcat ttaagctttt aaatggctat agaggaagtg
     7861 agccttgtga tattgatgct cttgcagata tgatggtcaa attaggaaaa tatgcttatg
     7921 aaaataagga tgaagtcaag gaaatagact taaatccagt gtttgtatat cctaaaggaa
     7981 aaggcgtatg tgcagtagat gccttaatag ttaaatataa atagtttata agttttggta
     8041 ttcaaccata attaaagcac cgactgaaaa tcacattttg taagttagat agtgtgattt
     8101 tcagtcaccc caaatttaat ttaaaaatat tttaaaatga aagtatagtt ttgagtttaa
     8161 ttgtgaaata gcactgtata aacaataaaa tactacgtat aaaacgaata ataaaaggtg
     8221 ttttaatgat aagaatcaat cttgattttc attaaaacac cttctttatt atattaaaaa
     8281 tttttaaacg ttatattgtc acaggtgggt ttgttggagc tgcatatgta cgattagcaa
     8341 attcttggtc aacaaagaat agaccaccct ctccagcaag tttaacacgt tttatattgt
     8401 cttcacaatt tgtttcttct tctgcttgtt cagatataaa ccaaagtaag aattgagaag
     8461 ttctaaagtc tgattcagag tttgctacag ctgctaagtt gtttattaaa gctgtaacct
     8521 ttttttcatg agctaacgta cgctcaagaa tatccattgc agaagtaaag tctgaatctg
     8581 gcattgggat agcatcaagc tgaacctttc ctcctacatt atgtaagtat ttgtaaaaaa
     8641 acatagcgtg gtctgtctct tctttatatt gaacataaaa ccaattagaa aatcctttaa
     8701 gtccttctgc ttcaaggtaa gaagacatag aaagataaag atacgcagaa tataattcat
     8761 ggttaatctg ctcattaagt aatttttcca ttttagctga aatcattttt atacctccaa
     8821 attttaaatg ctcttataaa atataataat attaaatgga aaaatattca ataaaagtgt
     8881 ttaaatattt tacatattta ataaaaattt atactaccat taaaaaaatt ataacttaca
     8941 atggctatag agccaattaa tggtgcattt gctccaaaat ttgaatgcct tacacttatt
     9001 tttttatatt tagaaaaact tgctgaagca gtcaattttt ttaataatgt attttctata
     9061 aatgtaccag gtatattaaa gtcatagcct attattatag aactacaatc aatcaatgta
     9121 agtgtattaa ctaatgcata tgatacgtat gtacaaaatt catcaagcaa aaacatggca
     9181 agactatctt tattatttgc ctcatctatt aacttgacta aagaaaaatc tttacagtta
     9241 attaatgggg agttaggata aatatggctc aatgattcaa ttcttttaat tagattactg
     9301 acacttgtat aataatctaa acatccattg ttaccacaac tgcacaatgg tccactaaaa
     9361 ttaattgatg tatgtcctat ttctccactt tgtcctaggt tgccagtatg aagtttattt
     9421 tctagaacta gaccagctcc aataccattc attatgtgta ggtatatata atttgatata
     9481 tttttaccta aaccatacat tttttctgct aatgcacccg aattagcatc atttatcaaa
     9541 aatattggaa aatcagacat ttcttttata aaatctacta tatttaggtt tgatacattt
     9601 ccaaagtctg ggggatttag tatggtacca tttatatcat ctactggacc tattgaagaa
     9661 ataccaattc caattatatc tctcttgttg attttcatta ttttagtaaa cattcttttt
     9721 attatatctt ttaaaacatc atttgataga catttagggt aagtttcaga aagttgtttt
     9781 acaatattac cttttaaatc tgaaataatt accttacata tatttcttct tattaggatt
     9841 ccacaaatac aaggcgatat attagaaata tctaataaaa taggtttacg acctgatgaa
     9901 tgtgcattta aagttgatga ctctatttcc ctaatcatat tttcattaat aagttcagta
     9961 actagatttg ataaagtcat tttagtaagt cctgtagact tagctaaatc aactctggac
    10021 ataggtgtat ttgtagaaag taatttcaat attattaact tatttttaat ttttacatct
    10081 ttgatgttag aaccttgttt gttactcatc ataaatctcc ttaaataaag ttaagtgttt
    10141 tatttatttt caagtatact atcaccttat aatagagtaa cattttttta cttgaaaata
    10201 tcattaatta gaatatatca cattatatat cataaaacat ataaaaaaat gttagaatta
    10261 aattaaaagt gaaaagtaga ttgatatata aatagtaaat acttaattaa aaaaagataa
    10321 tgatagcaat aattattgtt accaagaatc tactacttca agtgttacct taggtattta
    10381 agttgatagt ggtttgcacg taataaactt gatgtaatat atattcttaa taatatcgat
    10441 aaaagattta cttaaaaata atatagatta tgtaaacata taatctattt attgtataca
    10501 ataaaatttc attttgtaaa acagtgaatt aaatatgtaa tatagatatc gatttttatt
    10561 acaaattgtt actaaatata ggtacattag aacaaaaatc tagtaaattg taactaaaat
    10621 gtaaattttt cctttaatct atacaatatt atggaaactt tttgtaattt atgatataat
    10681 aacaaatata taattattaa attataataa atgactatag cgtagatgta atactatgtt
    10741 atagtcattt ttgaattaaa cttgatatat caagggggaa gaatatgaga acaattagaa
    10801 gagttttagc attaggttta acattagcta tatttctaat aaatgtacct aatgtagatg
    10861 cattaacatc agatacaatt aaaggtaaca atatatatga gacggctgga ttaatagcag
    10921 ataaaaagag ttatgataca gcaattatgg taaatatgga taattctata gcagatggtc
    10981 tttcagcaag tggtcttgca ggtgctgttg atgctccaat tttgcttgca caaaaaaata
    11041 agataccaaa tgaaacaaaa caaagattaa aaaatgttaa aaagatatac ataataggta
    11101 aagaattatc aataagtaag tcagtagaaa ctgaactaaa aaacacaggg gcacaggtaa
    11161 ctagattggg tggagatgac agaataaaga ctagttatag tgttgctaaa gaggtaaatg
    11221 gtatcaaaaa agttgatgaa gtgatattaa ctaatgcata taaaggagaa gcagatacaa
    11281 taagtgctgc acccgtatca gtaagagata tagctcctat agtacttaca gatggaaaga
    11341 gtgtgccttt ttcaacaagc aatgtaaaaa catatgcagt aggtgggagt atatcaatga
    11401 gcactagttt agttaataaa actaatgcta aaagacttgg tggttcagat agatatgata
    11461 caaataagaa agttataaaa gagttttatc cagatgcatc agaattttat ttgagtgatg
    11521 gatatgattt agtaaatgca cttacaggtt ctacaattgc taaggaaaat ccaattgtat
    11581 tggtgtcaga aagtagtgat aagtctatat tagcaggagc agataagatt actagattag
    11641 gttcaataag tgacagtgtg tataataagt gtgtttctgc tgcacaaaat aatggcgatt
    11701 cgtctacaaa aggtgagtca cctatgaaaa atgagacaag catattaggt cagccaacag
    11761 ctagtttaga agcgtgctta aaatgggcaa aatctaaaaa agctaatgat ttatttatag
    11821 agttaatacc aatattatat gatactgctg ttcaggaagg tgttaatcct gttttggcag
    11881 tagctcaatc tgcaaaagaa actggttttt gtaattttgg tggagtatta gatgcatcat
    11941 ttaagaatcc ttgtggactt aaaacttctg taggtggttc tgatactgat aagaatgccc
    12001 attcaagatt tgatacttgg gaagaaggaa tattagctca aattcaacat ttatgcttat
    12061 atgcaggaca agatggttac ccactttcaa atccagtaga ccctagacat gaaaaatctt
    12121 tatttggtaa ggctaaaaca gttgaaagtt tatctaataa ttgggctgga ggtcaatatg
    12181 gacaagattt agtaagaatg atgggagaaa ttgaggcaac aaaataattg aagattatca
    12241 aatatccaat aaataaggat tgattattat gaaaaagagt aatattttta gtatcatatt
    12301 tcctacaatc ataatggtgc taatgttttt aacttttgct acagatatat ttaatattcc
    12361 agatattcaa tctaaaagta tttttgttgt gggaatggtt ttgatttttc caatttcttt
    12421 tttaatacaa gggataattt gtgtattaaa taagactaat tggattttat ctcttattgt
    12481 gtctttagta acttatatag tattaatgta tatattttta aatgattctg catatatata
    12541 tgttttactt tatacagctt tttatatgat tggacatata acagctaaac tctattacaa
    12601 aattaagact tttaaaaatt aaattgaagt atataaaagt aacattaaga gaagtatcct
    12661 taatgatttt ctaaaatcag aaataggtgc ttctcttttt tgtaaaaata aaagtatgta
    12721 aataaaattt aaccattata attttctttt aacaaatgta tacaataaac ttgttgtata
    12781 attaagatag ataaaataaa gggagggata atatgaattt aatagataat catattcata
    12841 ctaatttttc aagtgatgga aaggattcca tggaagatac aataaaaaaa gctataagta
    12901 ttggtgttag atatttgact tttacagacc atttagaaca tgatgaagat agaggatttt
    12961 gcataaatta taatgactat gttccagtat ttaataagtt taaagaaaaa tataaaaagg
    13021 atatagagtt attactaggt gttgaagtag gttacagaaa acatctaaaa aatgaaatag
    13081 aagaaataat aaattctaat ccttttgatt ttgtattgtg ctcaactcat acaattgaca
    13141 atgtacctgt tccatcaaag gcgtatttta aaggtcttag caaagaagat gcgtattata
    13201 aatattttaa tagcatactt gaaactaatc gtgaatttgg agattacaat atatatggtc
    13261 atttggatta tatatctagg tatggaatat attcagataa tagagttata tacaatgact
    13321 ttaaagatgt tattgatgag gtcttaaagt caataatcaa taatgggagt ggtatagagc
    13381 taaatacttc tggttataga tatggattaa atgctataca tccaaatgaa gatattctaa
    13441 aaagatataa agaacttgga ggatttatag ttactgtagg ttctgattct catagggtag
    13501 aagatatatg taaagatttt gatgtggcct atgatatgct taagtatctt gactttaaat
    13561 atgtatcttt atttaaagaa agagaaactt attttgtaaa tataaaaaaa gttaaatcta
    13621 acaatatagc ttagagtatg ttttgaagta aattaatgga tagttaaaaa gttaatagtt
    13681 ataatattgt atcctctaga aaaatactag aggtttttat tttgctaaaa taactaaata
    13741 g
//
LOCUS       DAMJVX010000095        13691 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMJVX010000095 DAMJVX010000000
VERSION     DAMJVX010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13691
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.099"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            29..1729
                     /locus_tag="PC407_002401"
     CDS             29..1729
                     /locus_tag="PC407_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497843.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            1899..2516
                     /locus_tag="PC407_002402"
     CDS             1899..2516
                     /locus_tag="PC407_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497844.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            2877..4070
                     /locus_tag="PC407_002403"
     CDS             2877..4070
                     /locus_tag="PC407_002403"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2497845.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            4088..5380
                     /gene="lysA"
                     /locus_tag="PC407_002404"
     CDS             4088..5380
                     /gene="lysA"
                     /locus_tag="PC407_002404"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDF2497846.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            5597..6256
                     /locus_tag="PC407_002405"
     CDS             5597..6256
                     /locus_tag="PC407_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497847.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KYSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(6411..7496)
                     /locus_tag="PC407_002406"
     CDS             complement(6411..7496)
                     /locus_tag="PC407_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2497848.1"
                     /translation="MIIILKENIKYYLIVVFIGILFFKFINTPSNFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            7687..8403
                     /locus_tag="PC407_002407"
     CDS             7687..8403
                     /locus_tag="PC407_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF2497849.1"
                     /translation="MRINKYIASCGIASRRKSEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            8461..9039
                     /locus_tag="PC407_002408"
     CDS             8461..9039
                     /locus_tag="PC407_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2497850.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            9433..10305
                     /locus_tag="PC407_002409"
     CDS             9433..10305
                     /locus_tag="PC407_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2497851.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     repeat_region   11136..11819
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=11136..11163
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaa"
     gene            11930..12214
                     /locus_tag="PC407_002410"
     CDS             11930..12214
                     /locus_tag="PC407_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497852.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGRICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(12984..13199)
                     /locus_tag="PC407_002411"
     CDS             complement(12984..13199)
                     /locus_tag="PC407_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2497853.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(13210..13677)
                     /locus_tag="PC407_002412"
     CDS             complement(13210..13677)
                     /locus_tag="PC407_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2497854.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFILENVKRFKVMGCCLLINTIIECVIG
                     YNSNTSLIMIFGDNSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT         5587 a         1402 c         2287 g         4415 t
ORIGIN      
        1 aataacattt tttagagagg agtattaaat gaaacgatat cttaaaacat atggagttgc
       61 attaataatt gtaataattt atgcatttat taaattacct gtactaagat tagattttac
      121 atctttattt tcagtaggaa taattttctt tgttgtagca ggaatactag atatgatgtt
      181 agatagaaat gaaagagcat ctaaaatggc taaatataat ttttcaatag caatagcttt
      241 gcttgtgtat attgtagtag ttccctttat aacttctaca cctgtacttc atgcaaaatc
      301 atatagagaa ctattgggaa aagtaactga aagtaaattt actgatgaca ttagccctgt
      361 cagtgtaaat gacattcgtc ttgtagatga agatatggca atgaaattag gtgacaaaaa
      421 gcttggagaa gttcctgcaa tagggagtgt atccaaatta ggaaaatttc atatacaaaa
      481 tgtagatgga gagttgtact gggtagcgcc tcttgttcac agagacataa ttaaatggat
      541 aacatctcta agtggtacaa gtggttatgt aaaagtatca gctagtaatc ctcaagacgt
      601 ccaattagtt caagaaatag atggaaagcc aataaagata gtttaccaac cagaagccta
      661 cttacatcag gatttacaaa gacatttata catacatggt attgtaaatg ttggtatgac
      721 agatttcact cttgaaataa atgatgaagg aagaccttat tgggttgtaa gtttatatga
      781 acataaaatt ggctatggag gagcaaatgc aacaggaata gccactgttg atgcagaaac
      841 tggaaaaata aatgtttatg atgttaaaaa cacacctaaa tgggtggata gaatacaacc
      901 acaaagcttt gtaacagacc agattaaaga ctggggtgtt tatgttaatg gatttttaaa
      961 ctctgttata tcagaaaagg gagttttagt accaacagaa ggtacatcat tagtttatgg
     1021 caatgataac aggtcttatt ggtatacagg tataacatct tcaggtggag atgaatctac
     1081 aattggattt atgttagttg attctagaac aaaagaagct agattatata aacaaccagg
     1141 agctactgaa acggctgcaa tgaaatcagc tgaaggtaat gttcaagaaa aaaattatga
     1201 agcgacgttc cctgtaatgt ataatatatt gggtcagcca acatatgtat catcattaaa
     1261 agataaggct ggtcttgtta agagagttgc atttgtatct gttgaagatt ataatgtact
     1321 tggaataggt gaagataaga atgaagcatt aagaaactat aaggatgcat tagaatctaa
     1381 gggtaatgat ttaaaacttg ataatgattt aaaagatgag gtattagaag gcacagtgac
     1441 aagaataagc ccagatgtgc gaggtggtaa cactaattat tatgtaactt tagattcaaa
     1501 taaagatata atatttaggg ctacttcaaa agtatctagc gaattgccat tgactcaggt
     1561 tggtgataaa gtcaaaatat cgtattcaaa agaagaatct ggtgtaatag aaatgtcaga
     1621 atttgataac ctaaatattg caaactttga agataaaaaa gcaaaagaaa aaacaggaac
     1681 tgatgaaaat acagattcca aagattctga aaatcaaggt aatgtataat ttaaataaaa
     1741 agattgataa tagtatttaa catagtgata gctaattttt atagaattta attctataaa
     1801 aattagctat tttattttaa ttctttgtaa atttaatgtc ataaaattaa tataatatta
     1861 gtacaacttt gcaaataata cttcaaggag aatgtactat gacgaatatg gaaaaagaga
     1921 tattactttt aattagcagg gttagatgtg ttacagagtc tcaatttaga aaaatttatg
     1981 gagaccaaaa aagatacaat aaaaaaaatt ttaaaaaaac tttgagaaaa atgtgtagtg
     2041 aatatacact aaaaaaatat ccttgtgatg tggattatta taattataaa gacctctctt
     2101 atgtatatta tttaaacggt agtaaacttt ttaagggaga tgatttaata aaggctctag
     2161 taggatcaga attagttgtt agaatcaata ctgcaggttg tgatgtaaaa agattttacc
     2221 gaaatgttag tgtagatgat gtgaaatacg atttatttat tgaatataca gaccaatttg
     2281 gtaatataaa gcaagtttta gttgatgttg acttaaataa tgaaatagac atttctaaat
     2341 acgaaaatct ttctaaaaaa atagataaat ctacaattcc attttttaaa gtaccaaacg
     2401 ttttggtagt tacaccagat aatctatccg aattaaatga tttagagaga tatgataatg
     2461 agtatagcat aaattttata gatttaggat taaataaact atttaattgt atataagcta
     2521 actttagaaa agaaaactat cgatttgcca cattttgtaa aataattgtt gcaatattat
     2581 atagaatatg ataaaactat attaataaat taattaaaaa taatgagcct agggtagagg
     2641 tagctgtatt caatagtatt caataggagc tggtaagcat tgataattga tgaaagggta
     2701 tatagccgaa gaaattttgt tgacggacaa attttctgga cttatgcaga atatgcataa
     2761 ggctgtcact tttaaagtgg tgagctacaa ggttacttgg tctatgaaat ttacacgtcc
     2821 aaagtatacc tttgggcgtt tttatttatc aaaatttagc ttgaggggga gaaagcatga
     2881 gtataattgt tcaaaaatat ggtggaagtt cagtagcaga tactgaaaaa ataaaatcaa
     2941 tagcagaaaa tataatagaa cgaagaaaag aaaatccaca aatggtaata gttgtgtctg
     3001 ccatgggaaa atccactgat gaatatataa ctttagcaaa ggaattgagt aatgaaccta
     3061 gtaagaggga attagatgct ttaatgtcta ctggtgaaat gatttcagca tctttattat
     3121 caatagcatt aaatgcactt ggatgtaaag ctataagtta taatgcttat cagttaaata
     3181 taaaaacgag tggtcttcat ggaaagtctc aaattgatga tataaacgta aacagaatta
     3241 aaaatagttt agatgaagga aatgtcgtga tagtaactgg atttcaggga atcaacgaag
     3301 atggtgatgt aaccactctt ggaagaggtg gttcagatac gtctgctgta gctcttgcgg
     3361 ttaaattaaa tggaaaatgt gagatatata cagatgttga tggaatttat tttacagacc
     3421 caagaaaata ttcaaaggct agtaaacttg atgagattga atatgaagaa atgcttgagt
     3481 tagcgagttt aggagcacaa gtaatgcatt caagaagtat agagcttgca caaaaatatg
     3541 gtgttgaaat ctatgtcgga cgtacatgtg gtacagaaaa aggaacatat ataagagggg
     3601 gaaaagacat gaaattagag gataaagtaa taacaggatt agctactagt gatgatgaca
     3661 gttctataac aataaaagat tttaaagctg aaaatatatc tagcttattt gaagatatag
     3721 caactatagg tataagtgta gatatgataa gccaaactgc acctatttta gataagataa
     3781 gtgtatcatt tacagttcca aaagaagagt tgggagaatg taaaaagata gtatctaaat
     3841 atacagatga agaacatgta gtgattgata ataatatcac taaattttct ttagtaggtc
     3901 ttggtatgaa aaatacttct ggagttgctg ctaaagtatt taagatattc aatgaaaatg
     3961 gtataatgat aaaacttata actacatcag agattcgtat aacttgtgct ataaattctg
     4021 atgataagca agtagctata gaaaaaatag cagaagtatt taatatataa aatttgggga
     4081 ggctattatg aaacttcatg gaactatgac tgtaaataat gatgaattat atattggagg
     4141 agtgagttgt ttagaactta gtaaaaaata tcaaactcca ctttatgtat ttgatgaagc
     4201 tttagttaga caaaattgta gagaatatat agaatatttt aaggctagag agggaaaaaa
     4261 taaggttgct tatgcaggga aagctttttt acctctttac atgtgtaatc ttataaatga
     4321 agaacaaatg tgtttagatg tagtttcagg aggcgagtta tatacagctt ataaatcaaa
     4381 ttttcctatg gaaaaagttt tctttcatgg aaacaataag actaaagaag aaataaacat
     4441 gggattagat ttaggtgtag gtaaatttgt agtggataat ttttatgaat tagatttgct
     4501 tgaagcatta tgtgaagata aaggtaaaac tcaagagata tactttagaa taactcctgg
     4561 tatagaagct catacacatg attatataaa aactggacag atagattcta aatttggatt
     4621 tgctttagta aatggagatt tatttagagc aatagaaaaa ttaagtgaat ataaaaatat
     4681 taaacttgta ggtcttcacg ctcatatagg ttcacaaata tttgatgtag aaccatacat
     4741 agatacagtg gatataatga tgaacttggt aaaagaaata aaagaaaaat tcaacataca
     4801 actaacagaa attgacttag gtggaggagt tggcgtttat tatagcgaag gagataaacc
     4861 taaaactata aaagagtttt gtgaaaccat aatcactaaa gcagaaactt catgtaaaga
     4921 gcttggaata gaagtaccta cattggtaat agaaccagga agatcaatag ttgcaaatgc
     4981 aggaagtaca ttgtatacag taggttctat aaaagatata aaagatgtta gggtttatgt
     5041 aagtgtagat ggaggtatga cagataatgt aagaccttcg ttgtaccaag ctagttatga
     5101 gtgtagtatt gtgaataaaa ttaatcatga ggctatgaat catgtaacaa tagctggaaa
     5161 atgttgtgag agtggagata tattaattgg agatataaac ataatggata ttaaaagtgg
     5221 agatatactt ataacaacat caactggtgc atatggatat tcaatgtctt caaattataa
     5281 taaaatacca agagcagcag tagtatcagt atttgatgga aatgataaga taatttgtaa
     5341 gagacaaagc tatgaggact tattagcttt agagatataa aatggaaaag tatataataa
     5401 aaattaaata ttaaagttta ttatgagcag atttgctagg atatgtatct ctaaattaat
     5461 ttttggttta ttgtatcgtt cgtagcatat ctgcttattt tttgttttac taacaaattt
     5521 cttgtaataa atataaaata ttttaaaaac tttataatta tgataaaatt taaagtatat
     5581 gacaaggagg gatttaatga gaggaagagt aattttatta atatcgatat tatctatatt
     5641 tcttgtagga tgttcattta ataaaaaaga tgaaataaat ttagtagagc aaggaaaaac
     5701 atatttagaa aaacataact ataaaaaagc tatggaatca ttatcctctg cactggaaga
     5761 agatagtact aatgagaatg caagagctat gtatatgcaa gctatgagaa tgtcaaatat
     5821 gacggagttt gaagaattaa aaaattatga gggtgcaatc aaggaactta aacttataga
     5881 gaaaatcaaa aatggttcct ctgttattaa agaagaggca actaaaaaga aagaagagtt
     5941 aactaaatta gaaaaagaac aaaacgaagc agaggaacaa agaaagaaaa aggcaaaaga
     6001 tacggctgga gaagataggt atagagttga atcagatgca aggaaatctt cttatagtgg
     6061 aagtagtaaa aataataaat atagttctaa aaataatgat aaaaaatcag atagtaataa
     6121 gtctgacaat aataacagtt cttctcaaaa tcaagaaaag ccaaataata gtaatccaac
     6181 accagcacca actccacaag atactactgg tggttcagat agtggtggaa attctcaaaa
     6241 ctcaaataat caataagtat aaaatatatg cataaattta atgaacaaat tatatattat
     6301 aaaaaatatc tcttagcaat tttttcttac taagagatat tttttgagaa ataatttaat
     6361 aaataacagt taaattttaa actaaaaatt aaagtcacag tagagataag ctattgtaaa
     6421 cgattatcta tatatttatc aacataagtt tttacaacag cagctattgg tactgaaaga
     6481 aaaactccta tgaaaccaaa tagagattgt cccaccaaaa tagcagatat tacccatata
     6541 gctcctatac caacttgatt acccataact tttggttcta taaaatttcc atctaattgt
     6601 tgcaatataa atagaaatgc aatgacccat atagtttttt gtggactata taatatagtc
     6661 atggctattg gagggattgc accaataaaa ggaccaaaat agggaattat attacctaag
     6721 aatattattg aagctaaaaa taaagcattt tctattctga taacaaattg aaatcctaaa
     6781 aatgcaatta taccaataat aagagagtct aatattctac caagtaaata atcatagaaa
     6841 atatcatgag tagtcctaaa aaattctata atgttatctg ccttgtttct agcagtagaa
     6901 gaatatagaa atctcttaaa tcccaatgca attttttctt tgtcatatat aatgtatatg
     6961 gatagtataa atcccattgt catattaaat agcatagaag atatactaag tgtatagatt
     7021 aagaagtttt taggaatttt accaacaaaa ttacttgctt cttttaataa attatctaaa
     7081 ctatgttgta tatgaggaat taaagttttt agaaaatcta tgtttgacat atttttttct
     7141 ataaaactat ctatataatc agtatacata ggcatttcat ttatcaatct atttaatgta
     7201 ttagctattg atggaattaa tagcctaatg gcaaaggcta aaataaaacc tataaatata
     7261 taagataaaa aaatggctaa aagtctatgt gctttgaatt ttacctcaaa aagcatcaca
     7321 agtggattta aaagtaaagc aagtaaaata gctaataaaa atgggctaaa aaactttaag
     7381 aatccttcaa tactagatat aaagtttgat ggagtattaa taaatttaaa aaataaaatt
     7441 ccaataaaca cgactattaa ataatatttt atattttcct ttaaaataat aatcacttcc
     7501 ttaaaatagt gtttagtcag ctattacata ttaatagtat gtgaaaagaa attacaatta
     7561 tttaggaaaa ttaaataatt tttttgcttg tttttaatta aggtctagta tagtataata
     7621 atgtagcaaa attaacattg ataaaaatat taaacacaca taatttaaga taaggaagga
     7681 ttaagtatga gaattaataa gtatatagct tcttgtggaa tagcatcaag aagaaaatca
     7741 gaagaaataa ttcttggagg aagaataaag gtaaatggaa gtgtagtaaa agagttatct
     7801 tttaatattg atgaagaaaa agacatagta gaatttgata ataaaaaagt aaaaccaagt
     7861 gaaaattata tatatatagt attaaataaa ccagagggat atataactac agtaaaagac
     7921 caattcaata gacccagtgt aatagacata cttaaagatg taagggaaag agtatatcca
     7981 atagggagat tagattatga aactagtggt ttattaatac ttactaatga tggcgattta
     8041 acatataagc ttacccatcc aaagcatgaa attgataaaa cgtatgtggc aagtgtgaag
     8101 ggaattataa gtgatgatga gataagacaa ttcgaaacag gactaaaaat agaagattac
     8161 acaacggcac ctgccaaaat aagggtaact aaagaaaata aagaaaaaaa ttattcagtg
     8221 tgtgaaataa ctatacatga gggtagaaat agacaagtaa gaaaaatgtg caaagctata
     8281 aatcatccag ttttaaattt aagaaggatt tctgtgggga aaatagtatt aaaagacacc
     8341 aaagttggag aatatagata cctaacagaa gatgaaataa aatatttaaa atcaataaaa
     8401 taagataaaa ataaattgag aaaaaatgaa ataaagttaa tagtagggag aaatagtatt
     8461 atggcatatg caaagtattt aactaaaatt acagatataa ataaatttta tttagaaaat
     8521 attataaatg aaggggacat agtaattgat gctactatgg gaaatgggta tgatacaagg
     8581 tatctagctg agaaggttgg agaaaagggc tttgtatatg cttttgatat acaagaagaa
     8641 gcaataaagt ctacaagaaa gaaactagag aaagaaggat atacagatag agtaaagttg
     8701 atatttgatg ggcatgaaaa aatgaatcaa tatataaaag aagaagtttc atgtgtttta
     8761 tttaatttgg gttatcttcc aagagcgaaa cattcaatta taaccaaacc agatacaact
     8821 ttaaaggcta taaagttaag tttagaaatg ttaaaagaaa atggagtaat aagcattgcc
     8881 atatatacag ggcatgatgg aggtcaagaa gaaaaagatt gcatatataa ccttgtcaat
     8941 aatttaaatc aagatgaatt taatgtttta gaatctaagt ttttaaatca agtcaataac
     9001 ccaccacaat tgattttaat tgaaaagaaa aaagcataat aaatactaaa aaacttgaca
     9061 agattctatt aagagttaat atatatagtg tgacctatta aaaatacttt aaataataag
     9121 tatacaacag cttattctca aaacaaaata tacatctaaa tatgcaaaat aagatagata
     9181 acttttaaat gagtaaaaat tgaaaaattt aaaagaaata ttggataaat attaaaaaat
     9241 gcaaggaaag atggtataat aaatttaatc gaaaatacta tgtgaagtat aaggtacaga
     9301 aaagttagaa ttttatgagt ttaagaagct atgataaaaa ctaagatgaa attgagataa
     9361 aaaataaaat ttttagagaa aaagacagtg ttaataaaac aaaatagttt atataactaa
     9421 aaggagaaaa aaatggaaga aacaaatgaa atgaaggaca gtaaacattt gatatcaaat
     9481 atacaatctc aatatacgag attgagtaag ggtcaaaaac ttatagcaca atacatttta
     9541 aataattatg ataaagttgc atttatgact gcatgtaaat tgggtgaaac cgtaggagta
     9601 agtgagtcta cagttgtaag atttgcaaat gcattaggtt atagtggata tcctaaatta
     9661 caagctgcac ttcaagaact cataaaaaat aaactaacta cagtacaaag agttgaaatg
     9721 gcacatgatt attccgatga ttttgcaata ctaaacaaag ttttaaaaag tgatatagat
     9781 aatataaggt ctacactaga agaaattgac gagagagcgt ttaaagaagc ttctaacaaa
     9841 cttttaagag cgagaaaaat atatatactt ggaatgagga gttcttttgt agtagctcaa
     9901 tatttaggtt tttacttaga tataatactt gacaatgttc atataattag aatggatatg
     9961 ggagatgctt ttgagcagat tgtaagaata aatgaagaag atgtaatagt tgctattagt
    10021 ttccctcgtt attctaagaa atcttatcaa attgtcaatt atgctaaaga aaaaggagcc
    10081 catgtaatat cattaacaga tagcttattt gcgccagtag catcacttgc agataacact
    10141 cttctagtta agagtaatat ggcttcattt gtagattctc tagttccagc tcttagtatt
    10201 tcaaatgccc ttgctatatc tgttggaatg aaggaaaaag aagatattaa acagcatttt
    10261 gatgatttag agcaaatttg gaaaagatat tctgtgtatg agtaaagttt gtatattaat
    10321 aagtattatt aattattaaa atgttcaaat atagtgatgt ttggataaaa tatataaaat
    10381 agctgactgg catttaactg acagtcagtt tttttattta aacaatattt tgcttaaatt
    10441 ttaaggaata tctttgtcta tttcttctaa atgagagtat ttattcatag ttattttcat
    10501 ttattatgtt tcttgttgat gatatatttt tttatagaaa agaaaatagt tatggttttg
    10561 tatctgttat ttttgcactt attcaattta tttttgtgaa acctggaaag ataaaacttt
    10621 aatagcagat aataaccttg agattgtaaa gtaacaaagt gaaaatgtag aaagagaaaa
    10681 agaaatggaa ggaagaaaag tatatagata aagagatata acacgtattt tgatttaact
    10741 gtataagatg aaaaatttga tgattttgaa tagtttaaaa aattgttaat gaaaataaaa
    10801 gacttgttga gctaaagtgc gattacatag gaaggatgca ataaaaagaa aaaggaatca
    10861 ttggaataga gaatataata aatacttata agaatgtaga tgcttttagt ttgtaaaatt
    10921 atcccatttt tattttatag tatgagtttt atgatataat aaaaatataa aagttttgca
    10981 gtgagcgatt tttgtgataa agtagggttt aatagttgaa atataaagcg ttgagagtgt
    11041 atgataactg ttatcaattg cactattgct cgctcactgc aagtttagga gaattgtata
    11101 tgtataagta ttggaaatac ttaatttatt ttggggtttt atattaacta tatggaatgt
    11161 aaagtaggat tggtgtgtta cgaccacaca acgctggact gttttatatt aactatatgg
    11221 aatgtaaaga gtgaatttgg cgaactcgat atagagaaaa aaataaaggt tttatattaa
    11281 ctatatggaa tgtaaaggaa gtttttaatg ttgtcgcgac tcttgctgtt gcttgtttta
    11341 tattaactat atggaatgta aagctgcttt ggcttttggt cgtataatgc tccttataat
    11401 agttttatat taactatatg gaatgtaaag ttaaatctag catcagtcat tagtataaaa
    11461 tttcctcgtt ttatattaac tatatggaat gtaaagaata aaaaaggtat agttttcttc
    11521 ttagttactg ccatgtttta tattaactat atggaatgta aagtgttata atattaaaac
    11581 acaactaaaa tatacagaaa gttttatatt aactatatgg aatgtaaagt ctagtttttt
    11641 ctctttagaa aaaataaaaa caactgtttt atattaacta tatggaatgt aaattaaaat
    11701 tgtgttttct ctttcataca cccacttcat tagttttata ttaattatat gtaatataaa
    11761 tgttttttct tattaaataa agattgagtc actttatatt taataataca tattctaaat
    11821 tgattttgtt tttatataag agaatttgaa taatatggaa aaagatgatt aataaattat
    11881 taattagggt ataaaatgat ataaatagaa taaataaggg gtggatgaaa tgcttgtata
    11941 taataaaagt ttttatccta atgacatatt tccaagatta gatttttcaa aaataaaaaa
    12001 acagttaaaa ttgatagata atgacctgtc agattttgga agaatatgta taatagaaaa
    12061 agaacattat acgataagtg taaacagtat aggtgaaata aatgtgtatt atgatttaga
    12121 gtacgaaaat aaggtgtata gaatagttta tgagattgaa aagttattta aatctcaagt
    12181 tggaaggttt agtatatcta catacagaaa ttgataatta aaaaagtaga aattaaaaaa
    12241 cttaatacta aagatataga taaatatata aggtaacagc tagagagagt tgttaccctt
    12301 tttaaatact aagtatatat tattcttatt aattttttgt aaattcacta aaagaccttt
    12361 ttatagaata ttattttctt tttttattaa ataggtaaat cagtaaaaat gcaattccag
    12421 ttccaattat tattccaatg atattttcta taattaattt ttttaatata aaagtaaata
    12481 ataaaaatgc tatacataaa cctaaaatat atcctttaaa ataggttgaa agttttcttt
    12541 tcatataatc accaacttgt atcagattta gtttacttaa ttagagtata acagtgttgt
    12601 aacttcaaag agtatctaaa agattacaaa tgttagataa atcatcaata atgtaattta
    12661 taacacgtag ctataaacta tgcaataata taaatggagg tataagtaaa tgaaaaagtt
    12721 ccatgttttg ctaacagttt aaataaatat taggtagaaa agaatgtata taggaatctg
    12781 tggcaaaatt atcaatttaa aagtatgatt tagtgtgata cagttcttat aggtaatgga
    12841 taacaaatag aaaaaggaac agtttctttc ttagaacgta ttttgttatg actttaaaca
    12901 aagttaatat taatgtcaat aatatagtaa aagaataaaa atcatatagt ataataaaaa
    12961 agagtgtttc taaacactct tttttaatca ttttcatctt ctacatattc aagtatatca
    13021 cctggctgac agtctaaagc tttgcaaatt tcgtttagag tggtaaatct aatagctttt
    13081 gcttttccag tttttaaaat tgataaattt gcatttgtaa tatctatttt ttctgccaaa
    13141 tctttaagtg atattttcct ttttgccatc attacatcta ggttaactac tattgccata
    13201 acatcacctc tatatcgtta aatcattatc ttcttttatt tttatagctt tatcaaatgt
    13261 ttctgcaatt acaaagcaca ttagtgcaaa tataaaagaa ataaccataa gtggtgagat
    13321 accgccacta ttatctccaa atatcattat aagactagta tttgagttat aaccaattac
    13381 acattcaatt atagtgttta tcaataaaca acatcccatt actttaaacc tttttacatt
    13441 ttcaagtatg aatggggaat acttagtact attaattata aatatcaaga ataagatgac
    13501 tataaagtag tcagaaaaat atagtattct tgcaattaaa gcgattactt tgtcagaact
    13561 aaaatcatat aaaaacatag gtatcataca taagaatatt ataccagcaa ctcctaaaag
    13621 tgataataga aaatatagaa atactgataa tatattacta aaaaattttg taatcataaa
    13681 atatcatctc c
//
LOCUS       DAMJVX010000096        13688 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMJVX010000096 DAMJVX010000000
VERSION     DAMJVX010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13688
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.100"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            43..1836
                     /locus_tag="PC407_002413"
     CDS             43..1836
                     /locus_tag="PC407_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2497855.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDGIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILDELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(1917..3299)
                     /locus_tag="PC407_002414"
     CDS             complement(1917..3299)
                     /locus_tag="PC407_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2497856.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            3557..3904
                     /locus_tag="PC407_002415"
     CDS             3557..3904
                     /locus_tag="PC407_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2497857.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            3922..4956
                     /locus_tag="PC407_002416"
     CDS             3922..4956
                     /locus_tag="PC407_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497858.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVVWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(5005..6054)
                     /locus_tag="PC407_002417"
     CDS             complement(5005..6054)
                     /locus_tag="PC407_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2497859.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENRVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            6329..7864
                     /locus_tag="PC407_002418"
     CDS             6329..7864
                     /locus_tag="PC407_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497860.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFNLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(8050..8736)
                     /locus_tag="PC407_002419"
     CDS             complement(8050..8736)
                     /locus_tag="PC407_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDF2497861.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEGVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            8861..10243
                     /locus_tag="PC407_002420"
     CDS             8861..10243
                     /locus_tag="PC407_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2497862.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            10645..11913
                     /gene="eam"
                     /locus_tag="PC407_002421"
     CDS             10645..11913
                     /gene="eam"
                     /locus_tag="PC407_002421"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HDF2497863.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(12006..12362)
                     /locus_tag="PC407_002422"
     CDS             complement(12006..12362)
                     /locus_tag="PC407_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497864.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(12513..13607)
                     /locus_tag="PC407_002423"
     CDS             complement(12513..13607)
                     /locus_tag="PC407_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2497865.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         5359 a         1722 c         2171 g         4436 t
ORIGIN      
        1 tatatttagt tattcaaact attatttcga ggaggtcttg ttgtgaatat aaaagataga
       61 ttaagtggat taagaaaatt tatggaagaa aaaaatatag atgcctatat gattccatct
      121 tctgataatc accaaagtga atatgtagga gattacttca aatcaagaga atttatatca
      181 gggtttaatg gttcagcagg gacggttatt gtgactaaag acgaagctgg gttatggacc
      241 gatggaagat attttataca ggcagaatct caattagaag gaagtacaat aaaattattc
      301 aaaatgggac aagaaggttg cccaactaca gatgaatatt tatataaaaa tattcctgaa
      361 ggaggaactt taggatttga tggtagagtt atatctgcta gagaaggagc tacattagct
      421 gaaaaattat ctaaaaaagg tataaagata gaatatcagt atgatttaat agatggtata
      481 tggccagata gacctgcttt atcagatagt aaagcatttt tattagatgt taaatattgt
      541 ggagaaagtt ttagctcaaa attagctaga ttaagagaaa aaatgagtga aaaaggtact
      601 tctactcatg taataactac tcttgatgat atagcatggt tattcaatat aagaggtgga
      661 gatgttaaat ataatccagt tgtactttct tatgcagtaa taactttaaa agaagtttat
      721 ttatttgttg atgaaagtaa gttaaatgaa gaaatattag atgaactagc taaggaaaat
      781 gttcaaataa agccatataa tgatgtgtat gaatttgtaa aaaatataga caaaactgaa
      841 aaagttttat tagatggaac taagcttagt tatacaattt acaataatat accttgtgaa
      901 gttgagaaag ttgatgagtt caatccagta atgtttttta aagctcaaaa aaatgaggta
      961 gaattagaaa atataagaaa ttcacatgta aaagatggtg tggctttcac taaatttatg
     1021 tactggttaa agaaaaatgt aggtaagatg gaaatcactg aaataagtgc tactcaaaaa
     1081 ttagaggact taagaagaga gcaagaaggt ttctttgagc caagtttcaa tacaattgct
     1141 gcatataaag agcatgctgc aatgatgcac tatagtgcaa ctccagagtc aaattataaa
     1201 ttagaggcag aaggattatt tttagtagat tcaggtggac aatattatga tggaactact
     1261 gatataacta ggactacagt tttaggacct ataagtgatg aattaaaact acactttact
     1321 tctgtagcaa gaggaatgat aaatctttct aaggctaagt tcttgcatgg ttgtagagga
     1381 tataacttag acatactatc tagaagttgc atgtggaata tgggaattga ttatcaatgt
     1441 ggaactggac atggtatagg atttgtgcta aatgttcacg aagctccaaa tggatttaga
     1501 tggagagtag tacctgaaag atttgatagt gcagttcttg aagaaggaat ggtaactact
     1561 aatgaaccag gtatatatat agaaggttct catggtataa gaacagagaa tgaaattgta
     1621 gttagaaaag ctgaaaagaa tttctatggt caatttatgg aatttgaagt tgttacgtta
     1681 gctcctattg acttagatgg tatagtacca gaattaatga ataaggatga aaaagattat
     1741 ttaaactggt atcataagtt agtttatgat aaaatatcac ctttcttaac tgatgaagaa
     1801 agagaatggt taaaagttta cactagagct atatagtttg tactagatac aataaaaatc
     1861 gaggtattat aatgcctcga ttttttattg tatattttat taactttaaa aaatttttaa
     1921 tttgataatt ctactaattt tttattcatt ataagtagta taattccaaa tatcaataaa
     1981 aatgatggta tcactatgaa gacttgtaac ataccaagtt ggtctataat agcttgcgta
     2041 tagccagata atttatttgc tgcaaatttg cctaaaaacc aaacacccat taggaatgaa
     2101 atatactttt caggcgaaag tttatttacc ataccatacc cgactggaga aaagcaaatt
     2161 tctcctatac tttggaatgt taaaaatcca actacccaaa atacacttgc tttgtgaatt
     2221 gaattagggt ctgttcctgt ttgtataact gctactatca taaacaaaaa tgctatagct
     2281 aatagtataa aacctaagct catttttttt ggcacactta aatcgccttt tttagtttga
     2341 gataatttta cccatactaa agcagagata ggtccaagaa taacacaaag gagaccattg
     2401 tatgaatcta tccatgaagt aggaatttga aaattgccaa cagtaagatt tatgtgttcc
     2461 tttgtataga gtgcaataga catagaggct tggttatatg ctatccaaaa aaatactgtg
     2521 aaaaaagcta acaccaatat tactattatt ctttttttct caatcaaagt taaaggtctt
     2581 gaagcgagca ttttcttata ttctagtgat tttcttatag actctctccc gttactaggg
     2641 tcattattca aaggtttata aaactttctg tttccaaata taaagaagaa taaacctatt
     2701 agaaccgtaa ttgatgaaaa taaaaataca tatctatatc catacatact tatagataat
     2761 ccgcttttca tagcaaacca ttcattagca actaaaccgc atattaaagg gccaaataaa
     2821 gtacctagat tagtagccat atacgtaata gagaaagctc catcttttct tgggtcatca
     2881 ttggtatata agtttccaac catagtatgt aagtttcctt tataaaaacc tattcctaat
     2941 ccagataaaa atatcatgat gtttatatcc atcttactat ttgctatgta tcctagaaaa
     3001 taaccaattg aaaccattag cattccaagt ataatacagt ttttgggacc taaaaatcta
     3061 tcactgaccc atccaccaat aactggagag aaataagtcc atgctaaaaa agatgcaagc
     3121 attgatgcgg attctgctgg tgttaaccct aaaccacctt tggagacttg ttcagctaaa
     3181 aacaataata ataccaattt cataccatag tatgcaagtc ctacaaaggt aaatattgaa
     3241 cagcagaaat ataaatctct aggttgttta agtttttctt gcgtttttaa aactgacata
     3301 tgtcgagaac ctccagataa atagtttaac aaaattttac catactatat gagtatatgt
     3361 ttttttcata tagacgtcaa cttgtaagat aatttaatta agtcgaaata tatgtaaaaa
     3421 tagtatcatt tttgtgtatt cagtaatatt tttttatgat aatatatgtt attattatat
     3481 taaaatacaa acatatttat ggaaaggata tgccaattta gtagttagat tgattgtttg
     3541 gcaggtggga tgagctatga taagaaagaa atctgaatta caagtagaag taaaagaaaa
     3601 tttaaatggt ggtataggaa aggttaagct tgaaaacata atacaaaatg aagagcttaa
     3661 aggaaaagga agactattta aaagagtagc tttaccagta ggaagttcaa ttggtgtgca
     3721 tgaccataca acagattttg aagtatatta tatactaaag ggaaaaggta aagtatttga
     3781 taatggggaa tttgtagaag taaatgaggg tgatgtagtg tatacagctg atggcgaaaa
     3841 acattctata gaaaatatag gagaagaaga tcttgagttt gtagcactag ttttatatat
     3901 gtaaaatatg gaggaggagt catgacaggc aagatagaag aaattaagga caagataaat
     3961 agattttgca actccaattt aaatcaagaa tacaaagata taagctttaa aattttgcaa
     4021 aaccttttag ataataataa agagataatt cattcttcta gagctgatat atggtcttca
     4081 gctatactaa atatagtttt agaacaaaat cttttataca ataagaagca tccactacat
     4141 ataacaaaaa aagagttttc aaaacaaata ggtgtttctt taaatactat aaataataaa
     4201 tcgtctttaa taaaagatgt ttgtagtatt gactttttaa atcaagatac tatagctatt
     4261 tctaatgtag aattttgggt aaaagagacc aattccaaat caaagtctgt agatttagat
     4321 ttagaaaaga aaaaaatact atataaaaga tatattaagc tatcacaaga tgcgaatagt
     4381 gatattgaat caattagata tatggaggaa gctgtaaata taggtaaaag tatgattact
     4441 aaagaagata aagaacttgg attttggaaa ggtttatcta ccagagccta tatgatagca
     4501 cttgaatccc ttgctagaaa gttagaaagt attaataatc taaaggagtc caaaaaagta
     4561 ttagaatatt taatagaaat taatcctagt gatgagcagg gaataagata caaattatta
     4621 aatgtattaa ttagattaaa tgatagaact tcaataaata aattatttga actttataaa
     4681 gaagaaaaat ctgctacatg gatgtacagt aaagctcttt attactttaa aaataaaagt
     4741 atgtttttgg caaatgattc tattaaggtt gctagaagta aaaataagta cgttggatta
     4801 tatttgttag attggcgaaa tgcttttggt agagaatttg ttacagagga agaaaaggca
     4861 gaagctgtct attactatga tgaaaacata gtggtttgga atgaagttaa aggttctatg
     4921 gattggctat taaaaaaaat gttggaattt tcataaaaaa aactactcct tacttttata
     4981 taagtaaaag gagtagtttt taatttatac atctagtttt aaatcttcag gttttattag
     5041 accttttttt ctcataaatt ctgaaatcaa caaagttaca atagcaggaa gtataaaatt
     5101 aagtaaaagt ataccaaccc acatagatat acctcctttt ccagcacttt gcatagcagt
     5161 tactgttcca aattgtccaa ccaatccaca agtacccata ccagaaccaa taggaatatt
     5221 ctccattttg aatattgttg tagataatgg tccaagtaat attgaagcta cagtaggtgg
     5281 tatccaaatt ttccaatttt ttattatatt aggtacttgc aacatagatg tcccaagacc
     5341 ttgtgccaca aggccaccaa ctctattttc tctaaagctc ataacagcaa agcctaccat
     5401 ttggcaacaa caacctacag ttgctgcacc accagcaaga ccacctaaac ttaacatcat
     5461 acaaatagct gcactactta taggtaatgt aagagctatt ccaacaagtg cagagactaa
     5521 agctcccatt attataggtt gcatagttgt agcttgcatt attatatttc caaatgaagt
     5581 cataaaagca gaaacagctg gaccaataag ttcagctaca agtactccaa ttagtattgt
     5641 cacagcaggt gtaaccaata tatctatttt tgtttctttt gaaactaatt ttccaacctc
     5701 tacagcaaaa agtgtagcga caaatgctcc aacaggtcct cctaattcat taccacaagc
     5761 tccaacaagt gcacaagaaa atagtacaag tggaggtgct tgaagtccat acgcaatagc
     5821 cactgctata gctggaccag taacctgttt agcaagaggg cttataactt cagtaaaaag
     5881 agaaatattg aaattatctc ctaatgtatt gaaaattgtt ccaattaaaa gggacgaaaa
     5941 aagaccaagt gccatataac ttagtgcgtc aataagatac ctttgaacag aaatttcaat
     6001 attttttctt ttagcaaatg atttgaaatt tcttttttta taattgattt ccaatttaaa
     6061 atcctcctca tataatataa gtataatttc tttttaatta attatataac ttttcagctg
     6121 acaagacaaa tgttatttca ctaaaattag actattgtaa tagtaagaaa ttaacattaa
     6181 ttttagtttt aaattttaaa tttatggttt ataattagta tagggatatt ctgaaatata
     6241 ataattttcc agtttatccc aatatttaac ttaggtagat tgcttacaaa acaaatgatt
     6301 atatattagg ctaaaaggaa gtggatagat gatatttgta aaaaataaga aatacagaaa
     6361 aattgccata attatatcta ttcctatatt gcttgttgcg cagtactacc tttttaaatt
     6421 tggaatactt agaccaatgg taaagggagt agaaattgaa attgtagatg gggaatatgt
     6481 taaagatata gataaatatg tagtaaaatt aaatgaaaca gttactttgt ctacaggtga
     6541 atatattaag gttccttcgt atgctaaaaa tccaaatatt tggtttaatg tattggataa
     6601 ctcagggact ttaaaaatag atggaaataa aatgacagca ttaaaggttg ggtattcttc
     6661 tgtgggtatc atgaaaaatt caagagtatt aaaaaaggct actataaaag tagtagaccc
     6721 agaggtagaa tcactagata tggagattaa tggggattta aaacatgttg gtgatagtgc
     6781 tactatagaa agtacaatag aagttgatta tgataagttt aaaaaatcat ataagccagt
     6841 ttataagagt agcaatgaaa atattttaaa aatcaaagga aataaagtaa gtgcagttgg
     6901 agttggtaaa gctacaatat atgctaagtg tggaaataaa gaaattgaga gaacatttag
     6961 gattcaagct agagttgctg aaataaatat ggatgatata aaaatagaag ttgaccaaaa
     7021 tgaaaaattg aaaccagata taataacttc tccacctggt ttagaaccac caaaaatata
     7081 ttatgagtta gtagattcaa aattacctgt agaaagagca ataagtttaa gtagtaatgg
     7141 aaatgttgta ggtcttagag aaggtaaaga tagggtgaga ataacttgtg gagataaatc
     7201 taaagtaata acagtaacag tagtcaaaga aactataacg aatgactata tacaaaactt
     7261 ggttggttca cataaaatag taaacaataa agttattatt aaacttgaat gggattatat
     7321 gaaagatgtc tttgattatg atatatatct taaagataag ttgtcaggag aatcatttaa
     7381 gaaaattaaa tctataacta taaatgaaag tgatttaggt aagtctaaca aggtatatgc
     7441 aactatagaa gtagatatga agggtaatca aaatatagat tttgatattt atgtcatagg
     7501 gaaaaaggat tcagatacaa ctaagccaag taatgtaata aatattacta atggaaatgg
     7561 aaattcaaat aatgataata atattaaaga tatgagggta gaaaatatat cagctaatgt
     7621 tgatagagat aataatgtca ttagagttaa ctggagtagt atatctcata aggactgtac
     7681 ttacagtata tatgtaaaaa ataatactaa tggagatgga gaatttaatc tatatcaaag
     7741 tgatatacag ggaaatgagc atactataag tattcctgaa ggagatttaa atctagatat
     7801 ttatgttgtt gcaaattata atggaaatag ctctcaaaat agtgatgtta taaatgtaag
     7861 atagatttgt atacaaattg aaagtgttga gtttatttaa atattaaatt aagaaaaagg
     7921 atagtatagg gtaattatac tatccttttt ttgttacaat atataggttt aaaatctcta
     7981 gtaatataaa ttataaaaag tcttatttac acaaatctat agatattttt tttattaatc
     8041 aatattgtct taaattgaac ttagtgcggc ttctattgca tgtattactg tcgtatcaaa
     8101 tagtggaata gaagtgtcat tttgtttaat taaaagacct atttctgtac atcctaatat
     8161 aacaccctct gcaccttgtt tgccaagctt atctataata gataagaaag ctttttttga
     8221 agattcagat ataattccta agcaaagttc gttaaatatt gtgtcattta ctattatacg
     8281 gtcttcttca tttggaatta agacttcaat tccattatta ataattacat ttttataaaa
     8341 atcttgttcc attgtatatt tagtacctaa taaggccact ttttttattt ctttttccct
     8401 taatactgta gcagttacat ctgctatgtg aagtaatgga atatgtatac tttcttgtat
     8461 tttatctgat accttatgca tagtattagt acagataaca ataaaatctg cccctgcctc
     8521 ttgtaattta attgcagcat cagctaaaat tttggcgctt ttttcccaat ttccactaga
     8581 ttggcattct tcaatttctt gaaaatctac actgtaaagt atacatttag aagagtgtag
     8641 tccacctaat ctttctttta ttactgtatt aataacttga taatatgtaa tggtactttc
     8701 ccaactcata ccaccaatta agcctatagt cttcatatta tacctctctt atttatttat
     8761 tttgaaatag ctatttacaa atacaagtat agcatctaaa atagaagcat acaataataa
     8821 aattgtatgg agtacaatta taattaagag gagtaattat atgccaataa attcttttga
     8881 aaactatcct atgacatgga aaccaaaatt agataaccga aaaccaccaa tatataaaac
     8941 tttagcaatg ttattagaag aagatattaa aagaggaaat ttaaatcctg gagataaact
     9001 gcctccacaa cgggaattag cagattttct agatttaaac ttaagtacaa taacacgagc
     9061 atttaaactt tgcgaagaaa agggactaat atgtgcaaaa gtgggaaagg gaacattcat
     9121 ttcttctgat gtaaatgtaa gtaatacatt attatatcaa acggaatcaa aagacataat
     9181 tgaattaggg acagttcatc ctccatatga gcaaaataca tatattattg actttattaa
     9241 aaatgtacta aaacaaccag aaatggatag gtttttacaa tacatgtctc catctggcac
     9301 atatatgcaa aaaaagtcaa ttgcaaaatg gatagaaaga aataatgtat acaccagtga
     9361 agaaaatata ttattatcta caggagggca aaatgctata tgtgcaactt tactaggatt
     9421 atttaaagct ggtgatagaa ttgctacaga ttctttaagt ttttctggaa ttaagtcaat
     9481 agctaaaatg ataggtattc aattagtccc aattcctcaa gaaaataatg aaatatcgat
     9541 tgaatatctt gaaagttatt gtaaaaatga aaatataaaa ggcatatatc ttataccaga
     9601 ttatcataat ccaacgacac atactatgtc tgatttatct cgtaaaaaga tagccaagat
     9661 agcaaagcaa tataatataa taattattga agatgctata aatagcattt ttagagacgg
     9721 tatacaaact cccatatttt ctttagctag tgataatact atatatattt ttagtacatc
     9781 taaattttta tgtgctggtc ttagagtagc atttgtagtt gctccaaaac gatatataga
     9841 aaatttagaa aatgcactat ataatatgaa tctaatggtt tctccattta cagcagaaat
     9901 agtacatcgt ttattatatt ctcctattat agataaaatt atagaagaga aaagagatgc
     9961 aattatagaa agaaatgaag ttgctgataa agttttgtca gattataatt tgattggtga
    10021 taagaattgc agttttcgtt ggttacttct tcctgatgaa ttggatggaa aatcatttga
    10081 aatttctgca aaaaatctag gagttcaagt atattgtgct gaacgatttt cagtaggaaa
    10141 ctctacagtt ccaaaagcag ttcgtatttg tgtaacagct ccaaaagatg tagaagaatt
    10201 agaaaaaggg cttaatataa taaagtcact tcttaagagt taaatttatt ttactattat
    10261 ttaattaaat aaataaaaag gcggagaaat ttattataat aaacctccgc ttttattatt
    10321 taatgaaata ctttactttt tattaaaaaa tttaatttta ataatcttga aaatataatc
    10381 gttttatggt aaaataattc ttaaaaatag aaaaaattaa tcatttgtta aaaaaaatgc
    10441 ataatttatt gtaatttatt gacgaaggtt gaaaaatatt cataaatgtg cgaaataaca
    10501 gaaaaaatgc aaaaattaat tcaatttcat ttgttataaa atctatatgg caaaattaaa
    10561 tctgcctatc agaatgatat aaacacatca gaaaggagga cgctatcaaa taatacaagt
    10621 agttaacttt gatagcaaaa aattatgaat gaacaaacta gaatatcctt agagagagct
    10681 gctgaattaa aatcaaaaat tgatgattat attcaggcta gaaaaacgat taacagaggt
    10741 cttgaaaaag aagaagagat aaataaacga aaacagaaaa tattaagtat cttaaatgga
    10801 actgaagagg attggaataa ctacaaatgg caattatcaa atagaataac agatgtagat
    10861 actttatcaa aaattataac tctaactaaa aaagaaaaag aatatataaa agaggttggt
    10921 actcaattta gatgggcaat atctccatat tatttgagtc ttatagaccc agaagatata
    10981 tgtgacccaa taaaattact gtctatacca acacatatag agttggaaga tgagcaagaa
    11041 gatttggacc caatgggaga agagtataca aacccagcag gatgtataac tagaagatac
    11101 ccagatagat taataataaa tgtaacaaat gagtgtgcta tgtattgtag acactgtcag
    11161 agaagaagaa atattggaca acaagattct cataagtcaa aagctattat ccaagaatct
    11221 atagactata tcagagaaaa tgaagaaata agagatgtac tagtaactgg tggagatgct
    11281 cttacattaa aagatgatta tttagagtgg attcttagcc aacttaaaga gataccacat
    11341 gttgattatg ttagattagg tactaggact cttgttacaa tgccacaaag aattacagat
    11401 gaattttgca atatgctaaa aaaatatcac cctgtatata taaatactca ttttaatcat
    11461 ccaatggaaa taactaagga atctaaagaa gcttgtgaaa agttagcaaa tgcaggagtt
    11521 ccattaggaa atcaggcagt attattaaat ggaataaata atgataaatt tgtaatgaga
    11581 tgtttaaatc aagaattact aaaaataaga gtaaaacctt attatatatt ccaaagtaaa
    11641 catgtaaagg gaacaaaaca tttcaataca tcagtagatg atggtcttga aatcatggag
    11701 tatttaagag gatatacatc aggaatggct ataccaacat atatagtaaa tgcgccaaaa
    11761 ggaggaggaa agactccttt gcttccgcaa taccttgtat caaaaggaac agattacgtt
    11821 atgcttagaa catgggaagg aaaagttata aaaatggaag atgaacctgc tgtagatata
    11881 aagaaactta taaaagagca agcacaggat taaaaaagta tttaaagttt ataaaaagaa
    11941 acaactttaa aataaaaaga aaatggatgt caaatttata tcaatttgca tccatttttt
    12001 attagttatt tattattttg ttgttgatta actaattcaa gatatccact tgggtggaag
    12061 taatatatct cagaagtatt ttcatttaca aagtaaccaa tatcaccatc tgcatctggt
    12121 aaaaatacat aaccattaag atttttagct tttaatgctt caaaatcact agcatttcct
    12181 tgatatatgt aatcaacttc attgttgtat tttactaaaa gttctttagc ttgttcttga
    12241 gtcaatttag tattttggct tacaggaaca ttcttttgag aatctgcata agaagattca
    12301 tttactggtg cagttaataa caaacaagat aaaactaata aagatataag tgcttttttc
    12361 atactaaaac cctctttcat gtttaattat ataagattca ttacttaata atattgtatg
    12421 tttcctataa ttaaatatta ccattaaaga gaataatatt acagttacac agctgtaact
    12481 tttgattaca aaatcgtaat taaattgtat ttttattcca tttgtatttt atcatgtcta
    12541 cgatttacaa actcacagaa gtatatcttt ataacgccca tcacaggagc agatagaatc
    12601 ataccaggta taccaaagaa tcctccacca acagtaactg ctaggattgt aaagaatggg
    12661 ctaagaccta gttgtccacc tacgattttt ggctctataa atgtaacttc aacttgttga
    12721 attattatta agtaaattaa agaagttaaa gcaattgttg gattggagaa taaattaatt
    12781 attacaactg gtgccatacc tattactgga ccaaaatatg gaaccatatt catgacaccc
    12841 ataagtgtac caaataatag tgcatattca gattttaaaa agtaaagacc aattccagaa
    12901 agtactccaa ctataaaaga atctaaaatc ttacctgtaa agtatttacc aatatttaaa
    12961 tttaagcttt gacatagttc tattataaag tctccatatt ttttacctag ggaaatataa
    13021 actacttttt ttgtaaacag taagaatttt tctttttcta ataatatata gaaacaaatt
    13081 ataaaaccta aaagaaattg tactataaat tttccaactg aaaatgtagt gctaaatatc
    13141 ccaccaagtg aaccaataag caagttacta aatttaggca ttacactcat aattttgtca
    13201 ccatactctt ttaatgtatt agggtcaact gattgtaaac tcttacctaa gtcgaataag
    13261 aactgctctg ttttatctat gtatgtagga atttgagcaa ctaaatcagc taaactattt
    13321 actatggatg gaactgtaaa taatataaag gaaattagta taactatgag tacaccataa
    13381 gtaagaagta acgaatatat tctcttaaaa tttaatttac tttctaagaa ctttactagt
    13441 gggttgaata tatatgctaa tacaaatgct attataaatg gtgttagtag tgacattaat
    13501 aaacttatca ctccaaaaaa atatttgtaa ttatcaataa ctttaatcaa gatatatgaa
    13561 acaactacag tgagtacaat atttagataa atttttttat cttgcaaaat aacacaacct
    13621 ttcttatatt tattaaattt agatatactt aaatctaagt taaagttaat atttatagct
    13681 tattatac
//
LOCUS       DAMJVX010000097        13383 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.101, whole genome shotgun sequence.
ACCESSION   DAMJVX010000097 DAMJVX010000000
VERSION     DAMJVX010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.101"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            220..1533
                     /locus_tag="PC407_002424"
     CDS             220..1533
                     /locus_tag="PC407_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2497866.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTIISLGGIFITLLGLPVYYYMKKR"
     gene            1705..2880
                     /locus_tag="PC407_002425"
     CDS             1705..2880
                     /locus_tag="PC407_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDF2497867.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMKLY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINVDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            2983..4002
                     /gene="hrcA"
                     /locus_tag="PC407_002426"
     CDS             2983..4002
                     /gene="hrcA"
                     /locus_tag="PC407_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDF2497868.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            4032..4652
                     /gene="grpE"
                     /locus_tag="PC407_002427"
     CDS             4032..4652
                     /gene="grpE"
                     /locus_tag="PC407_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDF2497869.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            4744..6591
                     /gene="dnaK"
                     /locus_tag="PC407_002428"
     CDS             4744..6591
                     /gene="dnaK"
                     /locus_tag="PC407_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDF2497870.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLADEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            6802..7956
                     /gene="dnaJ"
                     /locus_tag="PC407_002429"
     CDS             6802..7956
                     /gene="dnaJ"
                     /locus_tag="PC407_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDF2497871.1"
                     /translation="MSTKRDYYEVLGISKDAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            8922..9857
                     /locus_tag="PC407_002430"
     CDS             8922..9857
                     /locus_tag="PC407_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2497872.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     AEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKDYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            9893..11266
                     /locus_tag="PC407_002431"
     CDS             9893..11266
                     /locus_tag="PC407_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2497873.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEGNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(11454..12221)
                     /locus_tag="PC407_002432"
     CDS             complement(11454..12221)
                     /locus_tag="PC407_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2497874.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFYFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(12251..13360)
                     /gene="ugpC"
                     /locus_tag="PC407_002433"
     CDS             complement(12251..13360)
                     /gene="ugpC"
                     /locus_tag="PC407_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDF2497875.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDKIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
BASE COUNT         5151 a         1515 c         2353 g         4364 t
ORIGIN      
        1 aaatttaatt actttaaaaa attatagaag aaaatactta tttagataaa ataaataatt
       61 tttagagaat attttagaag ttacgattgt atgattttta aacgcatagc atttactcta
      121 ggactataga ctaatataaa aaaaaacttt gggaatacta aaaccataaa tttataaaat
      181 taatatttta tatactgtaa aacagtgagg agggacagta tgcatactca actacaaaag
      241 actattggtc tttcagcggc tctttctact gttgtaggta tggttatagg ttcgggagtt
      301 ttttttaagc cacaagcaat atacactaca actaatggag ctccaggtct aggtattata
      361 gcgtggcttt taggtggttt tataacaata actgctgggc ttacagctac agaaatttca
      421 gctgctatac caaaaactgg tggtatgatg atttatatcg aagaaatata tggagaaaag
      481 ttaggctttt taactggatg gatgcaaact gtattatttt tcccaggtac atcagcagca
      541 ttaggagtaa tatttgctca acaagcatct gaacttttag gaatgagccc aaataatatg
      601 gcaaatgtac ttcctatagc aattggagtt atactatttt tagcgttatt aaatataata
      661 ggttcatcct taggaggaaa agttcagaca gtagccacta ttggaaagat gataccttta
      721 attttaataa tagtctttgg atttattaaa ggtcaaagta gtgaagtatt aaatccattt
      781 gtaggagatg gtgtaaatgc atcaaatgca cttggacaag tattaatagc tactttgttt
      841 gcatatgatg gttggataaa tgttggagct atatcaggag aaatgaagtc tccagagaaa
      901 gatttaccaa gagcaatagt tggtggttta tcattagtta tggcagttta tattataata
      961 aatatagctt atctttgggt agtaccagca agtgaattag caacagttac ttccccagct
     1021 acattagtag caacaagatt gtttggaaat ataggtggaa aagtaataac tgtgggtata
     1081 ttaatatcag tatttgggac attgaatgga tatttattaa caggttcaag gataccttat
     1141 acattagctg aaatgggaac tttaccagca tctaaaactc ttttaaaagt aaattctgga
     1201 ggttctcctg taaattcaat actacttata actgtgttag cctgtgtata tgcattatct
     1261 ggtcaattta atcttttgac agacttaact atattttcaa tatgggtatt ttatgtattg
     1321 acttttatag gtgttatgag gcttagaaga caaaagccag atttacacag accatataaa
     1381 gtccctttat atccaataat accaattata gctatactag gtggactgtt tgtaataata
     1441 aatcaaatat tgacttcaac aattatatct ttaggtggaa tatttataac actattaggt
     1501 ttaccagttt attattatat gaaaaaaaga taactactta atttaagtta gataaatttg
     1561 ggtaaaggtg aaaattaact tttatccaaa ttttttatac tgttatacaa tataagcatt
     1621 atatattact tgatgtgtta ttttgtttga tttataattt agtaattgat gctttaagat
     1681 aatttaagaa atgaggaata aaaaatgcta ggactttatg tacatattcc attttgtgtg
     1741 aaaaaatgta agtattgtga ttttaattct tataaaatgg acatagattc aaaaaaaaga
     1801 tatatagaag acttaaaaat agaaatgaaa ttatatagta ataaattata taaagataat
     1861 aaatataaaa ataaagaatg ctgtagtcta aataaaaatg acaaaataac gagtatattt
     1921 gtagggggag gtacacctag tatactaaca tctgatgaaa taagagaagt atttataagt
     1981 ataaaagaaa tgtttgatat agatgagaat gcagaaataa ctatagagtg taatcctggg
     2041 acattaacat tagaaaaatt aaaaactatg aaagagattg gcataaatag acttagtata
     2101 gggctacagg ccattcaaga aaagcatttg aattttatag gaagaataca tacttatgaa
     2161 gaatttgaga aaaactataa agatgcatta agtgtaggtt ttaaaaatat taatgtagat
     2221 ttaatgtaca gtcttccaaa tcaaactcta tgtgactgga aggaaactct tgaaaaagtt
     2281 gtacacttaa atccaacaca tatatcagcg tattccctta tactagaaga aggaactgaa
     2341 ctatataata tgtatgaaag taataaattt gagcttatag atgaaaatgt agatatagaa
     2401 atgtatgagt atacaataaa ctacttaaag tcaaaaggat ataatcaata tgaaatatca
     2461 aattactcaa aagaaggcta taattgtgag cataatattt tatattggga atgtgaacat
     2521 tatataggga ttggagcagg agcttctggc tatattaatg agaacagata caataatgta
     2581 gaatctttag aagattatca tttaagtttg gtaaaaagag aaaaaccaat acaagaaaat
     2641 gaaattcttt ctgagaaaga tatgatagaa gaaaagatat ttatggggct tagaatgaat
     2701 aaaggcataa aatttgaaga ttttaaaaaa aaatttggaa tagattttag agagaagtat
     2761 aataagcaaa tagaaatgct attagctaga aagcttataa atcagagctt tgaaggtata
     2821 caattaactc aaaaaggaag agaaatatct aatagtgtat ttatagaatt tatggaataa
     2881 tttttaaggt gttgacaaaa taaatttata atggtatata atatatataa tctttttagc
     2941 actcgaactt agtgagtgct aacagctagg aggtataata atatggaatt aaatgaaaga
     3001 aaattaaata tcctcaaagc tatagttaaa gattatatag aaacagctga agctataggt
     3061 tctagaacaa tatctaaaag acatgattta ggtgttagtg ctgctaccat aagaaatgaa
     3121 atggctgacc ttgaagaatt agggtattta attcagcctc atacttctgc gggtagagtt
     3181 ccatctgaaa aaggctataa gttatatgta aactctctta tgagtaaaag cgaattggat
     3241 gataatgata agattcttat tgaacaatgt atgaatcata atataaatca tataaaagaa
     3301 ttgattcatg aaacctctaa gctattatcg caactaacaa actatacaac tgtagcagtt
     3361 actaagagtt taataaatca aagtgttata aagcacatac agttggttgc tatgaatgac
     3421 aataatattt tacttatagt tgtaacagat aaaggggact taaaaaaagc taaccttaca
     3481 actaatgtat atttagatca atctaagtta aatttgattt ctgataatct tacaaggaag
     3541 cttttaggta aaagtataac agatttggat gataatctaa ttgcttttat taaatacgaa
     3601 ataagtgagt attctggact tatagatgag cttttaaatg ctttgaactc aaatatgaaa
     3661 gaagaagatt tttctctatc attaaatgga gctactaaca tatttagtta tccagaattt
     3721 aatgatgtat taaaagcaaa gtcattttta aatatgttgg aaaaaaagga aacaatagct
     3781 gacataatta aatcaaaggg aatacaaaaa gacaatctaa atataataat tggtagtgat
     3841 aatgattgtg aactggcaca ggattgtagt atagttacag caacttataa tgtagataga
     3901 gacttggttg ggcgaataag ctttataggc cctaccagaa tggattatgc tagaatttac
     3961 tctataataa attatatgag tttattaatt aatagaaagt aaagaaattt taaattgcga
     4021 ggtgtaaagt cttggataaa aaaaatgagc aacaggaagt aagagaagaa aatgatactt
     4081 ctattaacca agaatcggaa actcaagtag agttagaaga ggaagttgtt aatgaagaat
     4141 gtgaaacatc aagtgaaaaa actgatgaaa aagaagttga tgatgaaaat gtaacagata
     4201 taaattctaa gttagcagaa aaaaaattgc aggatgagtt ggatgaatta aatgataaat
     4261 atcaaagact tcaagccgaa tatgcaaact acagaagaag aactcaacaa gagaaagaga
     4321 caataggcgt atttgcaaat gaaaaaataa taactgagtt gataccagtt atagatagta
     4381 tggaaagagc cttagatgca tgtgaagata aggaagatac tatgtataag ggtataagcc
     4441 ttgtacataa acaattaata gacactttgg taaaatttgg agtagaagaa atagaagcag
     4501 aatctaagga atttgaccct aatctacatc tagcagttat gcaagaaagt gtagatggag
     4561 ttgaagcaaa tcaaatagta atggtacttc aaaaaggata taagctagga actaaagttg
     4621 taagaccttc aatggttaaa gtttcttgct aagtataaat taaaggttaa attatttaac
     4681 ctaatataaa atattaaaat aagtaaaact taattatata attactcgag gaggatttta
     4741 gtcatgggaa aaataatagg aatagattta ggaacaacaa attcatgtgt tgcagtgtta
     4801 gaaggtggag aagcacaaat aatagcaaat agtgaaggta tgagaactac tccatcagta
     4861 gtagcattta caaaagatgg tgagagaata gttggagaac ctgcaaaaag acaagcagtt
     4921 acaaatgcag ataaaacaat aacttctata aaaactcata tgggaactga ttataaagta
     4981 aatatagatg gaaaatcata tactccacaa gaaatatcag caataatatt acaaaaatta
     5041 aaatcagatg cagaaagtta cttaggacaa acagtaacag aagcagttat aacagtacca
     5101 gcttacttta cagatgctca aagacaagcg actaaagatg ctggtagaat agcagggctt
     5161 gatgttaaga gaataataaa tgagccaaca gcagcagctc ttgcttatgg tatggataaa
     5221 ttagaccaag aaaagaaaat attagtattt gacttaggtg gaggaacttt tgacgtatct
     5281 atacttgaaa taggagatgg aacttttgaa gttttagcta cagctggtaa caatagatta
     5341 ggtggagatg actttgacca aatagtaata gattatttag cagatgaatt taaaaaagct
     5401 gaaggtgtag atttaagaaa tgataaaatg gctcttcaaa gattaaaaga agcagcagaa
     5461 aaggctaaga aagaattatc atcaacaatg agttcaaaca taaacttacc tttcataact
     5521 gctacagcag aaggaccaaa acacttaaat atagatttat caagagctaa atttgaagaa
     5581 ttaactagag gtttagttga aaaaactatg gaaccaacta agacagcttt acaagatgca
     5641 ggactttcta caggtgatat agatgatgta ttattagtag gtggttctac aagaatacca
     5701 gcagtacaag aagctgttaa gaagtttata ggaaaagaac ctcataaagg tataaaccca
     5761 gatgagtgtg ttgcagcagg tgcttctata caagctggag ttttagcagg ggatgttaaa
     5821 gatttattat tactagatgt tactccatta tctcttggta tagaaactat gggtaatgta
     5881 atgactaaga taatagaaag aaatactaca ataccaacta agaagtctca aatattctca
     5941 actgctgctg ataaccaaac agctgttgat atacatgttc ttcaaggtga aagaagtatg
     6001 gcttatgaca atacaacttt aggaagattc caattaacag atataccacc agcacaaaga
     6061 ggaatacctc aaatagaagt tacttttgat atagatgcaa atggtatagt taatgtttca
     6121 gctaaagatt taggtacagg aaaagagcaa aaaataacta taacatcaaa cactaattta
     6181 tcagaagcag aaatagagca aaagataaaa gaagcagaaa tgaatgcaga agctgacaaa
     6241 caaaagaaag aaaaaataga agcattcaat caagctgaat ctactatata tcaaactgaa
     6301 aaaactttaa atgaacttgg agataaaata agttcaggtg aaaaagagga tatagaaaaa
     6361 gctatagcag acttaaaagc tgtaaaagat aatcaagatg ctacagcaga agaacttaag
     6421 aaagctacag atgaagtaat gactaagttc caaaaagtat ctcaagaaat gtaccaaaaa
     6481 gcagctcaag aacaacaagc ggcacaaggt gcagaacaag cacaagacaa tggacctaaa
     6541 gatgacaatg tagtagatgc tgactttaaa gaagtagatg aagataaata agctatataa
     6601 tatgtaacac taaatgataa ttggaacata tatgatttgt gaaaatcata tagatgttaa
     6661 agatatatgg acaatacaca tcataaagaa tataatatat catagattat ataacttaaa
     6721 gtagaatata agaattaatg atagcttgta gtttattatt ctacaagcta tcgttttgta
     6781 aaatgaaggt ggtgtcaatt tttgagtaca aaaagggatt attatgaagt cttaggtatc
     6841 agtaaagatg cagaagctca agagataaaa aaagcttata gaaaattagc aatgaagtac
     6901 catccagata gaaatcctgg agataaagag gcggaagaaa aatttaaaga gataaatgaa
     6961 gcttatgaag ttctatctga tgatacaaag agaaaaactt atgaccagtt tggacatgat
     7021 ggattaaatg gtcaaggtgg ttttggtggt caaggaggat ttggcggtca aggctttggt
     7081 ggttttgaag atatgttcgg agatatattt ggtgacatgt ttggaggaag cttcggaggt
     7141 ggaagagcaa gaagaagagg gcctcaacgt ggtgcagata taagacagag tgttactata
     7201 tcttttgaag aagctgcatt tggtaaaaag atgtctataa aagtaaatag aagtgaagag
     7261 tgtgaagaat gtaatggaac tggagcaaaa ccaggaacat caaagaaaac ttgttcaact
     7321 tgtaatggaa ctggccaagt tagaactgtt caaagaactc catttggtaa tatagcaagt
     7381 tcaagaccat gtagtgcctg taatggaact ggagaagtga ttgaatctcc atgtagtaaa
     7441 tgtcatggaa ctggaaatac aagaaaagtt aagactatag aagtagatat acctgctggt
     7501 atagatgatg gtcaaatgat aaaactttct ggtcaaggtg aagtaggaga aaaaggtgct
     7561 ccaagaggag acttatatat tgtagttaat gttaagtcac atccactatt tactagagat
     7621 ggaaatgata tttattttga aatgccaatt acatttgttc aagcaacttt aggtgatgag
     7681 atagaagtac ctactttgga tggaaaagtt aaatatagtg tgccagaggg gacacaaact
     7741 ggaactgtat ttagacttaa agagaaagga atccctagaa ttagaggtaa ttcaagagga
     7801 gaccaatatg ttaaggttgt agttgaaatt cctaaaaaat taaacgataa gcaaaaagaa
     7861 ctattgagag aatttgcgaa ggagtgtggg tcgaacgtcc acgaaaagaa gaaaaccttc
     7921 ggacaaaaga tagaagatat gtttaagaaa aagtagttaa agtagccaat atataatcat
     7981 atttttgaaa tcttagttat ttataagatt gtcaaaggag atattatgat ttttatattg
     8041 gctatttttg tataaaaaat aaatgtgcat aaaataaaac tgaatataaa taattttctg
     8101 aaaaatactt gtcctaaaat atataatgtg ctataatata atcaaataaa agaaaggggt
     8161 gattccagtg ggaacggatg tggcactgcc aaatgatgaa gaaaacttta ttgtaatata
     8221 caaaataaaa ttagatactt ttagtgctag attaagtact ggaaaaactc atgagctggg
     8281 ataattattg taattaccta gactcaaact tttagtagta aaatagtaat attatataga
     8341 caaggtatct aatttttaaa tcttttgtat gagttaaaat ttttataaag ggtatatctg
     8401 attagaatgt aattagggaa acctagttgt gaattacttt tagtatatac atgtgtaaag
     8461 aaatatgtat atggtattgg ttattgtgct agatgtgtat gtaaaggaat atgtgtctag
     8521 ttgggtatat gtaaagaaat atacttataa tctatgaaaa atttttagta taaattgcta
     8581 aaacattgag attgtttttt atagaaagtt agtttatacc aactaaaaat gtgcttaaga
     8641 tttgtttgat gtttaattat aaataaggtt gtagataaaa aaataagtaa agacaaaggt
     8701 ttaaaatact tgctttaaat atagtttgtt ttgaacactt gccaaattaa aataattaat
     8761 aaaatagaca tcttctcata gtaaaatata ctgtgagagg atgtttttgc tttgttcata
     8821 aacttttatg aaaaatttgc atagtgtcac attttaatat actatatata taataaaata
     8881 tagataaaat taaatgattc aaaagagaaa ggtgaaaaga gatgtattat ataggtgtag
     8941 acataggagg aacaggtata caagcaggag tagttgataa ctatgggaag ataattttta
     9001 ggtcagagtg taaaacagta attgaaaaag gctttgaagg aatacttaat gacataaaaa
     9061 taatgattta taagttgttg gaagataata aacttactat gagtgacata aaatctattg
     9121 gatttggtgt tccaggtttt ataaataaag aaggattagt aacttgtgta aatttaaaat
     9181 ggaataaaaa agcttttaat aaagagttaa aaagaagatt tccagatgct gaaattcatg
     9241 gagaaaatga tgcaacagta gcagcacttg gagaagcaaa gtttggaagt atgaaaggtg
     9301 ctaatgttgg agtattatat actttaggaa ctggtgtagg tggaggaata gtaattaatc
     9361 aaaaagtctt ttctggagca catggattgg gttcagaaat aggtcatcaa attataggag
     9421 aaaactattt taattgtaat tgtggaaata atggttgtgt agaaactttt tgttcagcaa
     9481 ctgcaattat aaagtattct caaaaactga tagaagaagg agaaaagagt aggattttag
     9541 atttggcaga gggaaattta gaaaatgtaa atgcaaagat ggtgtttgat gcatatagag
     9601 aaaatgattt agtggcaatt aaagtaataa atagatttaa agattattta gcaaaaactt
     9661 ttgcaaacac aataaattct ttagacccag aaattatatc tataggagga ggcatttcta
     9721 aatctagcga tataatactt gatggaatag aagatttggt aagaaaattt gtattataca
     9781 aaacagaaga tatagctaca attacatgtg caactttagg ttcagacgca ggaattatag
     9841 gagcagcttt tttataatgt aaatgaactg cttattggag gcgtgatgat atatgggtaa
     9901 aacaaatgct agaagaacgc tttttttgat agcaataggt tcaggaacta tgctgaatcc
     9961 tcttaattct tctatgattt cacttgcttt acatagtata caaaatgatt ttcatattag
    10021 ttttacaact gtatcttgga ttgtttcagc attttaccta gctagtgcta ttgcacaacc
    10081 agtttcaggt aagataggag atttaatagg tagaaaggtc ttatttttga gtggtttgat
    10141 gcttgtaatt ttatcagcat ccatagtacc acttgttcaa tcattcttta tattgatttt
    10201 tataagagta attcaagcaa ttggaacaag taccctatat ccatctggag ttgctcttat
    10261 acaaaataat ataaaagaaa gacaatcttc tgcattagca gttctaacca tttttgcttc
    10321 aactacagca ggattaggtc cgacacttgg aggattattg ttggacttag gaggatggca
    10381 tgctatattt cttgtaaata tacctgttgt tttggtttct atatgcttag ggtatttttt
    10441 attcccaaaa gaagtaaaag aaaagaaatc tataaaagaa actttaaaaa atgttttgtc
    10501 aagattagat attattggca tacttctatt ttcaattgca atgattttta tattactatt
    10561 tttgctatct atgaaggaat cttttaactt agaacaactt gtatttggta taatactgat
    10621 gtgtcttttt atttggcatg aattgagaac aaaattgcca tttatagata ttaagctttt
    10681 tgtaagtaat cctaaattgt caaaagttta cttgcaattt attatattaa atttctttaa
    10741 ttatattttg ttttttggtc ttcctagtta ttttcaagat gccttacatt atagtgcaaa
    10801 gtcgactgga ttatttatgc tatttatgtc tggtataggt atatttatat ctccattaac
    10861 tggaaaatgg atagataaat ctggaactag atttcctctt gttacatcat caatctttat
    10921 gtttattagt gcagtacttc tttcagtagt ttttatccat atacctgtaa taggaaaggg
    10981 tattatatta tcattagcag gtattagtta tggagttggt aatgtagccc tgcaatcctc
    11041 tatgtttgaa gaaagtcctc gtgacagtat agggacagct tctggtctgt ttcaaactag
    11101 tcgttattta ggttctattt tagcaactgt tttacttgga atggtatttg aaggaaatat
    11161 aacatcagaa caatttcaga tattaggtta tgttatggtg gtcttaggta caataagttt
    11221 tttattaaat tttatattaa agaaagaatt aagaaaagta gaatagtgga tttatggatt
    11281 taataagtct aataactgat aagtttaaaa atattgtttg taaaacataa ctggtttgat
    11341 atttattaat tctaagtttt gaagcataga gtactcgata agtatacaat attttgaatt
    11401 aaaattaagc agaaatttga actagtcttt caaatctctg cttaatttag tagttaaatt
    11461 tttttataga gaaaataaga tttgacactt agataaaaaa tcattgcatt ttcaaaattc
    11521 tttaaatcaa acccaagtat ctcattaatc ttttccaatc tatatactag cgtgtttcta
    11581 tgtaaaaaca tactagtaga tgtcttagaa atatttaaat cattatttat aaattttatt
    11641 cctgtatata gaagttcttc gtcaatagat ttaatcttat taaaatctat taatttattg
    11701 agtatattat cctcattaat taaatctaaa gttttatatc taataaggtc aaacaaattt
    11761 aaaaacttat tttcagcatt gtcttttata atatgaggta gtaaatcaga acataactgt
    11821 aatctaaaat aaaaatcagc tttactatct aattcaccaa gataaaaaat ataagtactt
    11881 tgataagttt cagtttcaat tatattttgt gtagttacat taatatctaa attatattta
    11941 gaatttacta taaaaaaacc attgtgttgt attatattta tatcttcaaa taaattataa
    12001 aaaatatttt ttaggtattc aaagtctata tgagattggt aaattaaata aaagttgcct
    12061 tttaaattat caagattatt tttatctaga aaatcaataa cataaatagc attattgaaa
    12121 gtaactaaat ggctactagc atctaacttt tggttttcat agggatagat atttattttt
    12181 ttattaagct gtctttctaa aaaatctttt aactcttcca taaagtctcc ttaaaaataa
    12241 gattatattc ttatcttata gctaaagtag tttctttgtc aaatatgtgt actctactat
    12301 tgtcaactga aagtgttaag tcatcaccaa cattaattct gtttttactg tcaaatctaa
    12361 ttgttatcgt attttcgcct agttttaagt gagcataagt ttctgctccc ataagttctt
    12421 ttatttcaat tcttgattta aaacaagtat ctggattaga tttaataaaa gtatcttcta
    12481 catgaatatc ttctggtctg atacctataa caacttcatt attaatataa ttgtctttta
    12541 ttaaatcata gtgtttttct ttattcaatt taagcttaat atgttcattt tgtgcataaa
    12601 ttttatcatt ttcttctatt aatattacat caataaaatt catctgtggt gctccaataa
    12661 atcctgcaac aaatatatta gctggataat cgtatacatc ttgtggagtt gcaatctgct
    12721 gaactactcc atccttcatt actacgattc tatcagccat agtcatagct tcaacttggt
    12781 cgtgtgttac atagataaat gtagttccaa gctgttggtg tagcttagtt atctctgttc
    12841 tcatagcagt tctaagtttt gcatctaagt tggatagagg ttcatccatt aagaatacct
    12901 taggatttct aactattgca cgtcctaagg caaccctttg tctttgtcct ccagataatg
    12961 ttttaggttt tttatttaaa agaggtagta agtctaatat tttagcagct tctttaactt
    13021 ttttgtctat ttcatcttta ggcaattttc taagtttaag tgcaaatgcc atgttttcgt
    13081 aaacagataa gtggggatat aaagcatagt tttgaaaaac catagctata tctctatctt
    13141 ttggttctat atcattgaca agtttatctc caatgtatag ttctccttca gagatttctt
    13201 caagaccagc aatcattcta agtgtagtcg atttaccaca accagatggt cctactaaaa
    13261 cgataaattc tttatcttta atgtctatat ttatattttt tactgcgtta aatccatttg
    13321 agtaaagttt tgatatattt tttaaaatta cttctgacat aatattctcc ttgatattta
    13381 aat
//
LOCUS       DAMJVX010000098        13326 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMJVX010000098 DAMJVX010000000
VERSION     DAMJVX010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13326
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.102"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(243..1124)
                     /locus_tag="PC407_002434"
     CDS             complement(243..1124)
                     /locus_tag="PC407_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HDF2497876.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSNKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            1580..2554
                     /locus_tag="PC407_002435"
     CDS             1580..2554
                     /locus_tag="PC407_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497877.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYKLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNHPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(2670..3701)
                     /locus_tag="PC407_002436"
     CDS             complement(2670..3701)
                     /locus_tag="PC407_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2497878.1"
                     /translation="MKKIRKLATKLLITTIVLVSGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNNKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPTVGAVAQTQKPALPGKHTMLPHQDGAK
                     VVGKFGDIKDKHVMYEGVRCVVINSSVNTVTFLGSKSNLDPSNLTIAVIGFMNTPYGM
                     FALAPEGEPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIEITDEKANIGL
                     GQIMYIRYE"
     gene            complement(4313..5164)
                     /locus_tag="PC407_002437"
     CDS             complement(4313..5164)
                     /locus_tag="PC407_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2497879.1"
                     /translation="MSCILKLKQIFEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(5181..6101)
                     /locus_tag="PC407_002438"
     CDS             complement(5181..6101)
                     /locus_tag="PC407_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HDF2497880.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDGIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(6211..7125)
                     /gene="murQ"
                     /locus_tag="PC407_002439"
     CDS             complement(6211..7125)
                     /gene="murQ"
                     /locus_tag="PC407_002439"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDF2497881.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNRVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(7224..8321)
                     /locus_tag="PC407_002440"
     CDS             complement(7224..8321)
                     /locus_tag="PC407_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2497882.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDMGFT
                     GSEKALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFDFKPSTK"
     gene            complement(8554..9900)
                     /locus_tag="PC407_002441"
     CDS             complement(8554..9900)
                     /locus_tag="PC407_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2497883.1"
                     /translation="MLDKFTEFLDSKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(9989..10297)
                     /locus_tag="PC407_002442"
     CDS             complement(9989..10297)
                     /locus_tag="PC407_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2497884.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEASVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(10558..10947)
                     /locus_tag="PC407_002443"
     CDS             complement(10558..10947)
                     /locus_tag="PC407_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497885.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSNKNTKKEMEKNQRTDYNIPTISFK
                     SGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRGFSL
                     GFFRLYILFVLSFAIIGYFWIYKGIHI"
     gene            complement(10949..12109)
                     /locus_tag="PC407_002444"
     CDS             complement(10949..12109)
                     /locus_tag="PC407_002444"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDF2497886.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAIIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDKNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGVHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(12539..>13326)
                     /locus_tag="PC407_002445"
     CDS             complement(12539..>13326)
                     /locus_tag="PC407_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2497887.1"
                     /translation="IDSYLIDGQSFEENLIKDIIENLKIKNNSDIETYKQSLKELDDY
                     IAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKINFLVNSILYSSRSNTMFEDV
                     FINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDVYTDSKCMSYVLDQIINNAIK
                     YSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRVFDKGFTGKNGRNQLYKSTGM
                     GMYFVKKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDYLSLDS"
BASE COUNT         4285 a         2350 c         1463 g         5228 t
ORIGIN      
        1 aaatttctaa aaaacaataa aattaagtta taaacaccaa tatatttcta ttatttcaat
       61 aagtatacaa ttagattaga atctaacgct agtaataaaa aattactttc ccaaaataga
      121 tataagcctc attttatcgt tcaaataatg aaaatatatt tgatttcatg caaaaagagt
      181 gcctaatgaa ctaaaaagtt cattttgaga cactcatttc tttatataaa tttatatagt
      241 ttttaatcgt tcgcattcaa ccactcagtt aagactttta tatatttctc attttcttca
      301 acgaatggca tatgtcttga atactcaaat aactcccact tactattagg tattctatca
      361 tacatagtct ttgcaatata tggcgaacat aaatctattg ctccactagt tactagacaa
      421 ggctctttta tttcatgtaa tctatctgta aattcatatc cagaaagagt tcctgtagga
      481 gaaaattcat tttgtcccca tcctacaata tatgcttcag aacctgattt tttaggtctt
      541 ctcaggcact ctggagaatc ttctgtcact tcacctgcac aatacatttc cataaatctt
      601 tctaatgcat cattatattc tttattagag taatctccag tatttactgc atcaagcaat
      661 gctttttggt caacctcact catatatgat attcttcttt tttgttcttt ctcccaaagt
      721 tttgcagaag aaagagtaga tgaaaggatg taagacttaa taccttttgg tttatattca
      781 atagcatacc aaatagcctg catacctccc caagattgac ctaacagatg aatttcatca
      841 agtcctagat gttttcttag ttcaataagc tcctctatcc aagtatctgc attaaataac
      901 tcaggatgcc cttctacaaa ggaatttcca caaccaattt ggtcatacat tattacttgt
      961 ctaccacttt cagctatctt atccaatacc tcaaaatagt tgtgtgtaga acctggacct
     1021 ccatgaagta atactaatgg cttcttccct tccgtatttt ctcctactat acggtaatac
     1081 gttttaaaac ctttaaaagg catatatcct tcagtaattt tcatatttat ctcctccaat
     1141 ttgtaattat aaacaataat caagttcatt aacaatacta tttaaaacct ctcatacgta
     1201 tttaattatg tcttttaatc tttataccta cttttataaa tagtataaca taaaagttaa
     1261 aaatctctat actaaaccaa ttataaaatt atattaacta tataatttta ttttttaata
     1321 atagtatata catatttgat tattttcatt tgctatttaa aatcaatttt aagttttctt
     1381 cttataataa acttcctctt tgtggtctca tgttagtctc taaatttaaa attttacaaa
     1441 aaattttcat aaaataaaat tatattgatt gtatctaaac aaatagggca ataaaataat
     1501 taagtatagt tacttacctt tcaagcaata ttagttttat gttagaattt tattatattg
     1561 atttacagga ggtttgcatt tgaataatat taattcactt aaagtaataa aattatctct
     1621 tggatgttct cttgctatat tcattgcttg gctattaaaa ttagaatatt caatggttgc
     1681 tggagttata gtactactta cagtaaagga cactaaaaga gaaactctta aaggctccat
     1741 tggaaagatt tatggttttc ttttatgtac tattttttca tatttatgtt ttaatatttt
     1801 aggatataat ttgacttcat tttcaatatt tatatttata attataccac tttgtttttt
     1861 gttaaatatt caagatgtta ttgctatgtg tgtagtcata gcatcccatt actaccttca
     1921 aggagaaact tctatcaaat ggattttaaa tgaagctgga atatttgcag taggaactgg
     1981 aataggtgtc ttaataaata tgtatattcc aacaaatata cataaaatcc atgaagttca
     2041 aaaaaagtta caggaagaag tgagtattgt acttatagac attgcagata ttattgtaaa
     2101 tcctaaaaaa gaaaatggat atagtagaga tttatataaa ttaaattcac ttattgacag
     2161 cagtatttct gaaacttacg ataatattaa taatacttta ttaagtgata ctagattctt
     2221 cttagaacat atggatatta taaaaagcca acgggatatc ttagaaaatt tgtatagtta
     2281 tgtatctcaa ctaaattcta caccaccaca agctcacatt ctatctgctt tcatacataa
     2341 aataggttat actgaatttg aagctgaaac aggcaatctt cttcttgaag aattgaagag
     2401 attaatgctt agtatgaaaa accatcctct acctgttgac agaacagagt ttgaaaacag
     2461 agctatttta ttcctatgct tgactgagtt aaaacaattt ttagttaatc gtaaacatgc
     2521 tcagatgctt agagataata atttttataa ataaaaaggt gagaaatcca gcagttgacc
     2581 tgactacaaa atatcagata taatattaaa tgcgccccta gtaagagggc gcgatgttta
     2641 taacttattc tactgtcatg attaaatcat tattcatatc gaatatacat tatttgacca
     2701 agtccaatat tagctttttc atcagtgatt tctattttaa tagtcacatc atttttttta
     2761 cagtactttg ctaattctgc accagaacga tttttaccac taaaatctaa agaaaatggc
     2821 ttatcatatt taaaaggata ttcaacaggt tcaccttctg gtgctaatgc aaacatccca
     2881 tatggtgtgt tcataaatcc tattacagca attgtaagat ttgatgggtc taaatttgat
     2941 ttactaccta gaaatgtaac tgtattcact gatgaattaa ttacaacaca tctaacacct
     3001 tcatacatta catgtttatc ctttatatct ccaaattttc caacaacctt tgccccgtct
     3061 tgatgtggta acatagtgtg cttcccaggt agtgctggtt tttgcgtttg tgctactgct
     3121 ccaactgttg gatatgctgt cttatcaaat ggtaatgggt ggctaagact cttgtatatg
     3181 atatcctttc ctgctgattt attagcatca atatctacat gatacaaacg cccatcacca
     3241 aaacgtataa gattccactg gtggtcattt gatgtgctgt gtacataata agattcaatt
     3301 cccatagcgt cacacaaagc tttcatgctc ctagccattc ctgaacatgc acctttttta
     3361 gtaaccaatg tataggcagt attcgctcct tctactgcat tccagtcaac atatgttgtg
     3421 ttatttttaa tgtgattata ggcaactaaa atcttagcgt ctgtagtcat gttctttgta
     3481 attttattgt tcataaaatc tgctgttgct tgtgctactg agtttagttg ggcttgtgac
     3541 agctttggac catatacgct attggttcca gtgacatagc tttttggtgt ttttgctgtt
     3601 acctctttgg ctgtcatacc ataaactgtc atactcatac ctgagactaa aacaattgtt
     3661 gtaatcaata acttggtagc taacttccta attttcttca tacttagtat tctcctttat
     3721 aaaatattgt tacttcctat tctctaccat taataaagtt catttgggat ttaaaaaggt
     3781 ggattgtcaa tatgtatgtt tcttcattta tacgttcgtg aaatatttaa atttcatgga
     3841 aaatctaata gatattttct agataatttg tatacaatac aactcgcaaa tcagggtgtt
     3901 tgctgttagt ttgagaaaat gatgatgatt aatatattcg gaatctttga cctataataa
     3961 ataccattgt cttcaatcat taataatggt aagagtaaag agaattatca ataatacagt
     4021 taaaatcaac tttgtataca tttcatacat tctctcccaa caacattata cttcttataa
     4081 tttgtatatt caatacgttt tctgggaaaa tataggatat atctataaaa atataaaaaa
     4141 ggagttgaac ttaaaatttg attaaaaaaa atcaatttta agacaactca tttgtattat
     4201 aaaaatgcta tatattatta ttaagtaagt caatatttta gatatttatc tatttcttat
     4261 tgacttacgc ttatagtcta acttatttgt ttagttgata tataacctag ttttaagcat
     4321 tattaatatt taaataatca agtatccttt ttgtttcttg tagtttctgt tctgcctcat
     4381 ttaagttttc ttgaattata gaaatatata ttatatctat caacgtaagc attgctattc
     4441 ttgaagaaat agctccttct ctaagccttt tttctatatt tggtactact agctttaaat
     4501 cactaatctt actaagatga ttgtcactat atttagttat acaaatgacc ttacatcctt
     4561 ttaattttgc attttctata gactttatta cttcttttga ttctccagag taagatattg
     4621 ctatagctat atcttcttta tctacatttg aagataccat aagttgtgta tgactatcta
     4681 aagaggtaaa tgcctgttta tttatcctta aaagtttcat ctgtaaatct agtgcaacta
     4741 aagccgaacc acctactcca aatatatata catttttagc ctttattatc tgttcaactg
     4801 cttcttttat tatatctttt tcattcaaca accttgtttg gttgatagtc tctatgtttt
     4861 tgtttgtaat tttgtttata acactatcta tattacaatc atagttttca tctacatatg
     4921 tgtattcatt ttctttgccc atcaaatctt tagctatctc aatttttaaa tccccaaaac
     4981 ccgaatatcc catttttcta gaaaacctaa ctatacttgc tgtacttgtt tttgagtttg
     5041 acgccaattc tgatacagaa agcttactta tagcctctgt attattaagt atgtaatcag
     5101 ctattttttt atctacttcc gttaaatctt caaatatctg ttttaatttt aaaatgcaac
     5161 tcatactcta ccacctctac ctatttacta taatatttct tcttagcaag taaacatgct
     5221 cctaaatctg gagtcaacac tggtctcact ataccaaatc tcgttttatt caaatatttt
     5281 ttaagtggct ctaatattaa atctcctgac ttaaacactc cacctgaata agataccttt
     5341 atctttgtac cttgttcaaa atgatgctct aatcctatta taagcctact taattcataa
     5401 gctgcatcat caaatatttt actagcagta ttatcacctt caataactgc ttttaaacat
     5461 atcgtagcaa actttgctat ttctattcta tctccttgta ttccatcatt tacatatttt
     5521 attatgtcat aatcattctc tatattaaga tgtcttttta ttaaatcata taattttgtc
     5581 tttgaacacc ttccatcaga ctgcattgtg aacattttta aggtagccat tcctatataa
     5641 tacgctgaac cttcatctcc taatacatag ccccatcctc cacatctgtg aagattccca
     5701 tctttatcta aagcctgtgc tattgaacca gtacctgcaa ttatatttat acctttttcc
     5761 ccatttaaag aacctgctaa tgctatttct acatcattac ctagagtata atttatatga
     5821 ctatatgcat tttttgttgc tacttctaat gcatacaata cttcttttat ttttccatat
     5881 ccagcaagtc caacaaatgc atacgttatt tcttctacat ttatcttagc atctttacaa
     5941 acttcttcca acccagttat taataatttc gttagattat caaatcctat ttgattataa
     6001 tgacaagttc ctttggttat ggttcctact atatttaatt cttcatctac taatgtaaat
     6061 gttgttttcg ttcctccacc atctacacct aaaaaataca tactatcctc ctacaatatc
     6121 tttaataatt ctttcaatac ttaatatcta aaaataacct tccaatgctg tttttttaat
     6181 attgaaaggt tatcagtact attatttatt ttacaatttt tctattgatt ttaaagcttc
     6241 acttatataa ccttcttgtc tatctaggat actctttgca ctatccttat ctaaaccact
     6301 ttttatcata aatatagcca atttaactct attatctgct tcttctaaga atttttctgc
     6361 aacgtcatat tttacatttg tagctatcat tattattctt tttgctcttt caaccaactt
     6421 ttcatttgat gtcttaacat ctaccatcaa gtttgagtat acttttccca tcttaaccat
     6481 agaagctgtt gttaacatat tacataccat tttttgagct gtaccacttt tcattctagt
     6541 tgaacccatt attacttctg cccctactat aatgtttatt ggtaaatccg cttttttact
     6601 catttcacta ttcggattca tagttattga tagtactgca caccccaatt ccttagcata
     6661 ctccattgca cctataacat aaggagtcct tcctgatgca gctataccac atataacatc
     6721 cttagatgta actcctctat ctattatatc ttgctttcca agttctttat catcttcagc
     6781 accttctatt gctttaaata ttgcttgatt tccaccagct attatagctt gaacaagctc
     6841 aaaatccact ccgtaagttg gaggacattc tgtggcatct agtattccta tcctcccact
     6901 ggtacctgct cctatataga ataatctacc tccctgcttc attctatcta ctattatatt
     6961 aacagctttt gctatactct ccttttgttc ttcaacagca taggctacag ttttatcttc
     7021 gttatttatt aattctatta tgcctaatgt atcttcttta tctatttgca atgtattctt
     7081 atttctacct tctgtaacta aattttctaa agtttttatt aacatatcat ttcctcattt
     7141 tcttaatatt aaatttttaa acacatttta actccaactt tcataattat atttttataa
     7201 taaattaata ttttcataat aaattatttt gttgagggtt taaaatcaaa cttttgccat
     7261 ggtttaatat aatctaatat aaataattct tcttttctta tatatcctac tacattcgat
     7321 ttaccactgt ttttcatatc agtcaaggct atttgtaact cccctgcata atgaccatat
     7381 tcactacttt caattactat atctcctctt tttattattt taggagtatt aaatagttca
     7441 aatttgtatc ctttatattt aacacgagat tgagtagacc ttatcagact gtcagatata
     7501 tcccctctat taaaatgtat ttcttcaaag agtatcttct tctcaacttc tcgtatattt
     7561 tcctctattg ctacatcaaa tgtaaccata tcttttctca tagaacctaa tacctttagc
     7621 tcctcatatg ttggataaca atttgatatt attatatcat ttatattatt catagctatt
     7681 atatgcttta cttgaatatc tataggtaga tttctgtgca tctcaattgt tggaagccca
     7741 tcagttacag gccatggtcc aaatgtggtt tcattttgac ttgttacaaa aactgctgtt
     7801 cttaatccat acttattaaa tctttctgta cattttataa agtgttctaa atttaatcca
     7861 ctatatctat gtggataaaa gttatgacat cctgtcaaat tttctttatt tggttggtag
     7921 tccattattg tatctatata attagtatta ttactcatat tgatttctat tttaagattt
     7981 tgaggattaa aagtcattaa ggccttttca gaacctgtaa atcccatatc aagccttatt
     8041 ccgtctgcct tcgtcttttt aaaaaaagtc aaatctttgt agcttatttc taattcacca
     8101 aatactctgg gatttacatc aagtattacc tcaaaacctt taaacttagc gtattcattt
     8161 atacttttaa attttttaat tatattttct ttagtatctt taactgataa taaacatgaa
     8221 aatatacgtg taaatccatt ttcagcagct ttatctatat aatcatatat ttccttagat
     8281 gttgtttttt ctggataaat tgatattcct aatcttccca tctgtgtcat ctccttatat
     8341 cctatatgtg tttaaattta tttcgaatta cacgaaactt ttttaaaaat ttggtgaaaa
     8401 aaagtttcat atatatttat aaaaaaagaa aagtttacct tttctttttt tataaacaat
     8461 caacaaaata tcttcgttac ttatttcata actgttatac tttgaaattt atactaatct
     8521 aatatagttt ttaatttcca atatagtttc taattaataa gcttcttgct cttcaacttt
     8581 ttcttcatga agcaatctct tatcgtacat cttgacaaat ggatagtaaa ttattattga
     8641 tatagctata cataataagt taagaactga agctctccaa tctccaccag tggctaaata
     8701 tgcccctact ggtcctggta atgtccaagg agcagtaact actactctgg atactaaatt
     8761 agcactcata gcaaaccatg aaactgttgc cattactatt ggtgccacaa caaatggtat
     8821 tattaatatt gggtttaata ctattggtgc tccaaatatt attggttcat ttatattgaa
     8881 tattgctggt attacagttg cttttcctaa agactttcca taagctgatt ttgctctaaa
     8941 tgcaagaagt aaagctaagc ctattgtagc tccagaacct ccaatccata taaaccattg
     9001 aaaaaagggt tcagcagcaa tacctggaac tgcttgaccc gcagcatatg aagctgtatt
     9061 agcatctaat aattgtatcc ataaaggtct tgccaatgaa cctattattg atacaccatg
     9121 tattccaaat gaccaaaaaa acgttattaa aaatattagt ataattactg atggtaatgt
     9181 gtcagtggct gaaactaatg gagttactaa tttagctatg aagccatgcc aatcaaaccc
     9241 taggaaataa gtaacaatag ccattaaaat tattattaat gcggctggtg tcagagcttc
     9301 aaaagacctt gtgactgatg ctggaactgc ttctggcatg gttatcttga atttagattt
     9361 gtcagtaaat ctataaatct caacacctat tatagatgtt acaatagcaa caaacattga
     9421 acctccacca agattagcca ttggcatttg ccatccttga ggaattgaat tataccaagc
     9481 taaaagttct ggactctttt caactatctt taataaatca ggggtaagac cctctattgc
     9541 agtaggaact attgtcaaaa ggaatgtagc tgtagctaat aaacctcctg tcaatccatc
     9601 taacttatat gactttgcca aactataacc tatgccaaat gtagaatata ctgatataat
     9661 aaaaaatgtc attctatatg gtagtaatat tgtaactgca ttttccttta gatactgagt
     9721 taccccccat gaatctggta atggaggaaa tgctaatatc aagaaaaatg acccaactat
     9781 tataagtggt aatgaagcta cgattccatc tcttacagct cttaagtgac gttgttctgc
     9841 caatctggcc atagaagttg ataatttact gtctaaaaat tctgtaaatt tatctagcat
     9901 cttttctctc cttaattaat atttattttt attatcatta aaatttttat aaaattaact
     9961 ttcttctaac ctaacaacac tgcaactact atttactcaa caactcttga acttgtttta
    10021 ataactttgg tcctcctaat ggactataag cttgtgcttg tattaatgca catggtacac
    10081 tagcctcatc agcaaaagct tttaaatagt caaatctatg tctgacttgt ggagctacca
    10141 tagcaatatc ccatccattt ttaacttcat tctcaaactc ttgagtacca acagccaaaa
    10201 catctatatc tattccattt tttgctcctt ctttttgtaa tgcattaact gctatagcac
    10261 ttgacatccc tgatgaacaa acaaataata ctctcatatt gattccccct gtttttttat
    10321 ttcttttcaa tcattatagc atgtattttt gattacagga atacgtttgt atatattttt
    10381 tcatatttta catttttaat tgtaatttta tttcatgcat ttatatcttg ctttaattgc
    10441 tttatttatc taatcattct tttgtctaaa attacacact acttaaaata ctcatatctt
    10501 ttcttaaaaa agaaaaaaga ctatctttta ttaacataaa atagtctcaa tactttttta
    10561 aatatgtatt cctttatata tccagaagta acctattatt gcaaatgata aaacaaataa
    10621 aatatacagt ctaaaaaatc ccaaagaaaa acctctctta gtttcaacaa agtatcttgt
    10681 atatgcaact gcaaaagatg taattaatga atttcctata acaactccaa atctattatc
    10741 tattccaaat atagataata acaaaggaaa aactacagta cttactagag atgaaactat
    10801 tagtattaat ccacttttaa aacttatagt tggaatatta taatctgttc tttgattttt
    10861 ctccatttct ttcttagtat ttttattact ttttgcattt ttataattat tatttttctt
    10921 tcttttcttt tttttattat tagacatgtc atttacctcc aaaaggttta ggtgttatat
    10981 tccctaaaat aactttattt ttagcccctg ttgcagatat tacattagaa aaagaattat
    11041 ttaatgtttc attgcccaag attacaaatg ccaaagcctc ctttgcatca gaagaatacc
    11101 catattcatc ttgagttaat acttcaacct ctaataataa ctcttttata tatcctatta
    11161 aagttagatt atgaactcct ccacctccaa ttaataaagt atctacatta acctttggaa
    11221 gtatgaagtt tttataatta taatatatag cttttgccgt aaacatagtt actgttgcta
    11281 tcaaatcata tttatctaca tgtttatatt tgttaagcat gttatccaaa tagacttgtc
    11341 caaatacctc tcttccagtg cttttaggtg gcgcttgact aagatactta tgactcatca
    11401 aatcttttaa catttcttca ttaattttac cttttgatgc aaagtatcca tttttatcat
    11461 actttctatt aaataatctt tgacatactc catctattat catatttcct ggtcctgtat
    11521 caaatgcaaa tacatcatct atgttacatg atttaggtat tatagtaata tttcctattc
    11581 caccaatatt ttgaagtgct aaatttttat ttttatcact atataataaa aattcactat
    11641 atggtactaa tggagcacct tctccacctg ctgctatatc cattactcta aaattagata
    11701 ttacctttgt atttgtttca tatgctataa ttgctggttc acctatttga agcgttgaag
    11761 atacaaaatt attataactt cttggtatat gaaagattgt ttgaccatga gatgcgataa
    11821 aatccaaatt tgctatatga aagtttgctt tatgacacac tgattttaca gctttactaa
    11881 ataaatatcc aagcttaaaa tttaagctac agattaaatc aacactcgat tcctcttcta
    11941 tacagcactt tcttatttcg tcttttatat ctttaggaat ttctagagtt ataaattcta
    12001 tttgcttaac tttagtattt ctaccattcc cactaatttc agctagtact gcatcaactc
    12061 catctaagga agttcctgac attaaaccta cactatacat agttatcatc cttttaagtt
    12121 tatagttatt cctccccaaa tcaaggtatt aagtttaata attttagaat taatcttatt
    12181 tcatctttaa ttgcgataat ttactcttac gaaattaatc gagttaatct tatacacgtt
    12241 ttttacctta gctaaatctt acattaattt attttcagtg tcaatgtaat gcatgtaatt
    12301 ttttttcaca aacaaaacat attctctctc caaaaaccat attaaaatag ttactacaaa
    12361 taaaaaacat accaggtacc tatctgatat gtttttcata tataataatt attattttat
    12421 aatatagata gaacatcatt taaggcacta attaaatctt cactattttc aagaactaaa
    12481 tttattctta ttgtatatgt cgatttgtat atgatgatag attttatagt ctaataacct
    12541 aactatctag gcttaaataa tccgatatat cataaaagta gatattaaaa attgtatatg
    12601 aaccaatttt cgaacatacc tcaatctcat gccctaaact atctatcatt ttctttacaa
    12661 aatacatccc cattccagta gacttataca attgatttct tccatttttc cccgtaaatc
    12721 ccttgtcaaa aaccctagat atatcttcct catttatacc tataccaaaa tcttttatag
    12781 ataataccac tccattttct aacttttttg aattaaattc aatcttacca acttcctttg
    12841 aatactttat agcattatta ataatttggt ctaaaacata tgacatgcat ttagaatctg
    12901 tgtaaacatc attttctaat tcattcaaac taacttctac attatttttt ataagcaaaa
    12961 atgaattatt ttttatagac atttttacca atttttctaa attaaattta tttataaata
    13021 catcttcaaa catagtgttg cttctactac tatacaaaat agagtttact aaaaaattta
    13081 tctttgcaac ctgattctta atatccaaac tatcttctat gctacttaac ctatctgtaa
    13141 ttatacttaa agaagatatt ggaattttta tttcatgaac ccatttagct atgtaatcat
    13201 ctaattcttt taatgattgt ttataagtct ctatgtcact attatttttt atttttaagt
    13261 tttctataat atcttttatt agattttctt caaaagattg tccatcaatt aaataactgt
    13321 ctattt
//
LOCUS       DAMJVX010000099        13004 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMJVX010000099 DAMJVX010000000
VERSION     DAMJVX010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13004
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.103"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            80..961
                     /locus_tag="PC407_002446"
     CDS             80..961
                     /locus_tag="PC407_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2497888.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(1169..2032)
                     /locus_tag="PC407_002447"
     CDS             complement(1169..2032)
                     /locus_tag="PC407_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDF2497889.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(2187..4121)
                     /locus_tag="PC407_002448"
     CDS             complement(2187..4121)
                     /locus_tag="PC407_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HDF2497890.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKAINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLSV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSLEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(4233..5246)
                     /gene="galE"
                     /locus_tag="PC407_002449"
     CDS             complement(4233..5246)
                     /gene="galE"
                     /locus_tag="PC407_002449"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDF2497891.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(5271..6140)
                     /gene="galU"
                     /locus_tag="PC407_002450"
     CDS             complement(5271..6140)
                     /gene="galU"
                     /locus_tag="PC407_002450"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2497892.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(6338..6658)
                     /locus_tag="PC407_002451"
     CDS             complement(6338..6658)
                     /locus_tag="PC407_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497893.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(6648..8639)
                     /locus_tag="PC407_002452"
     CDS             complement(6648..8639)
                     /locus_tag="PC407_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497894.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCDIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKNMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(8761..11304)
                     /locus_tag="PC407_002453"
     CDS             complement(8761..11304)
                     /locus_tag="PC407_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HDF2497895.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGAKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQAKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(11582..11916)
                     /gene="rnpB"
                     /locus_tag="PC407_002454"
     ncRNA           complement(11582..11916)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PC407_002454"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            12048..12725
                     /gene="srtB"
                     /locus_tag="PC407_002455"
     CDS             12048..12725
                     /gene="srtB"
                     /locus_tag="PC407_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HDF2497896.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKTSSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKNNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(12885..12959)
                     /locus_tag="PC407_002456"
     tRNA            complement(12885..12959)
                     /locus_tag="PC407_002456"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12925..12927),aa:Thr,seq:tgt)
BASE COUNT         4008 a         2286 c         1326 g         5384 t
ORIGIN      
        1 aataaaaatg taaaaatatc ttaaaggtta acttctacaa atatataaat aaaaatacct
       61 atgaaaggat gaacaaaaat tgaatttata tcacttacgc tattttgtta cattggcaca
      121 tttagaacac tatacaaaag cggcagaaaa tttatctata acacaaccaa gcttaagtca
      181 tgctatttct ttactagaaa atgaacttgg ggtggctcta tttgaaaaag aaggtagaaa
      241 tatagtcctt actaagtatg gaaaaatatt tttaaaagat gtggaaaaat cattagaaat
      301 tttagattca agtgtaaaat cattaaaaat aactggtact ggtgaaggac aaatagactt
      361 agcttttctt agaacacttg gtactgattt tatcccagac atagtgcata aatttttaaa
      421 atctaatcct gctaaatcaa tagattttaa atttcatact ggtgtaacta ctgacattat
      481 tcaaggtctt aaagaaagaa aatatgatat tgcattttgt tcgaaacttg aaaaagaaaa
      541 gggaattgaa tttatacctg ttgccaaaca agatttagta ctaattgtac catatagtca
      601 tccactagct gcaaaggata ctatagattt aaaagaaacc ataccttatc ctcaaatagt
      661 atttaatcag agaagtggtc ttagatatat aattgatgat atgtttaaaa aaataaatca
      721 acagcctaat atagtctatg aagtagagga agaccaagta attgctggat tagttgcaaa
      781 aaatttcgga attgctgtag tcccaaatat gaatatgctt agctttacaa aagtaaaagt
      841 aattcagatt attcacccaa gttgggaaag aaacttttac ttagcattta taaaagatag
      901 atatttgcct cctgcaatta aaaactttaa aaactttgtc attaaaaacg ctcaattata
      961 atttattaat aaaggggtta tttttaaata tcctctttta ttctttttat aaaataaaaa
     1021 aatctatact tttaaaagta tagattccta actgacagtc ggcatccgag tctcagtaat
     1081 tatcgttata caacagcaac tggattcagt tcctgaataa ctaggttatt tacattatat
     1141 cagatttttt taaatttgtc aattgtatct aacgcatttt ttcaagccat ggtccaacag
     1201 gctctacaat aggaacttct ttaaatcccc aatttcttat atcattcaca ctcgttggag
     1261 tatatatttt gtttctgaca gatttgaata ttttgttttt ttctccatat tgcatacact
     1321 ttggattagc ttctattaac cctcttcttg ctctgctaag aggattacat aaaatatctg
     1381 ctatttctaa accttctcta tagtcagcat cttctttttt ggcaattata aaacccttaa
     1441 ccttatctct taattcttca tctatatcta agtatattgt tccattatta aatacatcaa
     1501 aaaacgcctt ttgtactttt aagtttgaga catcatccct tgactctata actattcgtg
     1561 cagattctac agtttcatta tttacaaatg aataaaaatt ctgaagaaca tgtataaaag
     1621 ccacaacata cggttccttt gaaggtgcat aatatttctt taatctagct ttatctactg
     1681 ttactgatat tatagtacac tctaatctag atagaaataa aggcaactca tcatagaata
     1741 acttaatctg tgaatcatca aacttctcaa atgctcttga ttttcttgat atatctgtca
     1801 aatgtattcc tatgttactg gttccaaaac aatactcttt aaatgcatcc atttctttcc
     1861 tcaaagaaca tacataatcg ccttgactta ctgcatattt atattcaaaa attactcctg
     1921 tcaatgtaaa tgggtcttca tgatttatat cagctcttcc actttcatca ataaacatta
     1981 tatatttttt ctttataatc ttactcaccc tcttaacata tgatgatagc atctatattc
     2041 attttatatg agttttaaaa atattccaag atttttatat aacttaaact taattttata
     2101 ctaaaaaatt gagatgtaaa acaatcaagc ttttattgat tgaatttcac atctcaatta
     2161 taattatttt tcaatatata tttttactat tttgcaagaa tatccattaa tttatccatt
     2221 actatataag atactccgcc tcctatttga taaggagttt caactttttt atttgcaaat
     2281 actctttgtt gctctaatga taatcctgaa ttatccatta aaactattgg tgaatgtaat
     2341 tttacagcta atggtccagc tgataaagca tctactaaag catctgattt actaactaat
     2401 acagcctgta aatcagtatc tccatagaat ttttctatta ctttggcatt agtatctact
     2461 ctactatctc catatattct atttcctgaa atatcttcag tagttatatc attcattttt
     2521 gctattactg aatcgttaat tgcacttggt ccacctataa agtatgcatt ttctaatttt
     2581 ctttctgtta gccatttata agattcttct gtaattccat ctttattgac taatattata
     2641 ggttgttttt cttccccagc tttagaagct acagataaag catctgcttc cccatttcca
     2701 gaaaccatat atgcagtttt tatttcttct cgatttccaa gttcttcagc tattcttgta
     2761 gcagtttgat atctatcttg acctgctatt ctctttgtct ctgtgttgaa agtagttttt
     2821 atttcttgct ctaatttaga acttattgca ttttctccac ctatcaagat tactgtttta
     2881 ggtgctaata acttcatttc ttcaacagtt tcagttggta aattagctgt attagtaagt
     2941 agtattgttg agtcatagct acttgctaaa ggagttgctg ttataccatc agctattgca
     3001 ttaccattta ctaatattac agtatctgaa ctatcagcat tccagtttcc aactgaactt
     3061 atctttttag ctgtatcaaa tctatttgca cctataattg tatcccatgt aggatttata
     3121 gttccatctt cattaaaagt ataatctttc ccatctattt tttgagaacc agttagcatc
     3181 tttccatctt tgtctaaata gtaagactta ccatctattt gttgccatcc tgtctgcatt
     3241 attcctgttt tgtcaaagta atacttatta ccatctattt cttgccatcc tgttactgat
     3301 aaaccatcag atcctagata gtatttcttt ccattctttt cttgccatcc tgtttgcatt
     3361 actccatctg agttaaagta ataagttttt ccgtctatta ctttccatcc agatactctt
     3421 gctccatcag ttcctaaata atattttttg ttattaattg ctttccatcc tgtttgcatt
     3481 actcctgaag catcaaagta atacattttt ccatttactt ttagccaact attcttttgg
     3541 tatgttccat cagattttac ataatacttc ttaccatttt ctgttttcca tcctgtttga
     3601 tttagattta tcttatattt tgccgctatc tttacatagc tgtcattact ataatctata
     3661 attacatctg aacctacagg aacttggtca aataaccatc taacatcttt attgtgcatt
     3721 cttatacatc caccagaaat atgctttcct atagaactct cattattatt tccatgtatt
     3781 ccatatgtat ctccataagt tccttttaat gctaatccca tccatctatc tcctaatgga
     3841 tttcttggac ttcctccagg tatattacct ttataatatg gtctattttt tatcttgtta
     3901 actacttttg ttttccctgt tggtgtttct gacccctttt tacctgttgc aactctaaat
     3961 tcttttacaa gtttattatt tacaaaatat cctagagtat tctttcttga atttactata
     4021 atcacatgtt tactggctgc gcttgattct ggtacgccac atgcaaatac taaaactaaa
     4081 gtcagtgcta gtactaaata cttttttaga ctttttttca ctataacttc cccctctttt
     4141 agaaacatta ctacatgtgt ctaattataa cataaaatta ccaatacctt atatttacaa
     4201 atttgttaca tttatacata taagtatgta ttctactctt catacccatt aggattcata
     4261 ctttgccatc tccatgaatc ttcacacatc tcttcaagtt catacttagc tttccatcca
     4321 agctcttttt ctgcctttga agaatctgca taacacatgg ctacatctcc agcccttctt
     4381 cctacaatct tatatggtat ttcttttcca cttgctttag aaaatgcttt tactaaatca
     4441 agaacactat aaccctttcc agttcctaaa ttataaacaa caagtcctgg attttcttct
     4501 aatttttgta atgcttttac atgaccagca gctaaatcta atacatgtat atagtctcta
     4561 actccagttc catcatgtgt tggataatca ttaccataaa cactcaattc cttcaattta
     4621 cctactgcta tttttgttat ataaggcatt agattgctcg gaactccatt tggttcttca
     4681 cctattctac cacttttatg agcaccaact ggattaaagt atcttaaaag agctatatcc
     4741 aatgacttat ctgcttttga tatatctact aacatctgct ctatcataag tttagttctt
     4801 ccatatggat tggttacaga aagtgggaaa tcctctaata taggacaagt atgtgggtct
     4861 ccatatacag tagctgaaga actaaataca aactttttaa caccatattc tctcattaat
     4921 tcaaataaat ttaaggtact tattagatta ttgctgtaat attcaattgg tttttctact
     4981 gattcaccaa ctgcttttaa agctgcaaag tgtataattg attcaatatt attttcttta
     5041 aatacaacat gcatttcatc tttatttctg atatcaatat tataaaattt tactggtttt
     5101 ttagataatt ccttaattct atcaaccact attgagttgc tattacttaa gttatcaact
     5161 ataacaactt cataacctga ttctaaaagt tctattgctg tatggcttcc tatatatcct
     5221 gctcctccag ctactaaaac tgccattgta attacctctt ttctaatatt ttatttttca
     5281 cttacttttt ttctcatata gtccattaaa tcatctttta aattttctct ctttagagca
     5341 aagtctattg ttgcctctaa aaaaccaatc ttatctccca catcatatct tctaccttca
     5401 aagttataag cataaatagc ctcttgtttt ccaagagttt gaagtgcatc tgtcagttgt
     5461 atctctccac ctttaccagg tgtctgtttc tctaatatac taaatattgc aggagttatt
     5521 atatatcttc caagtatagc tatatcagat ggagcttctt caattgcagg tttttcaacc
     5581 atatctttaa ctttatatac tctatcttcg atatgtttta catccaatat accatattta
     5641 ttagtatctt ctttagctat cttttgaaca cctaatacag ttgttttata ctcattataa
     5701 gtatctatta attgttttaa gcacggaaca tctgaatcta ctatatcatc acctaataat
     5761 acggcaaaag gttcatcacc tataaaactt tttgcacagt aaattgcatg tccaagacct
     5821 tttggctctt tttgtcttat ataatgaata tttaccatat ttgatatgtc tcttaccatt
     5881 tctaacatct ctgtcttacc tttttgctct agttctaact ctaattcaac tgatttatca
     5941 aaatgatctt ctatagattt tttatttcta cctgttatta taagaatttc ttcaattccc
     6001 gattctatag cttcttctat tatatactgt aaagttggtt tatctactat aggaagcatt
     6061 tctttgggct gtgattttgt tgctggtaaa aatctggttc ctagccctgc tgctggtatt
     6121 attgcttttt taactgtcat ctgcattttt tcacccttct tcgtaaattt tctattgtac
     6181 caaattaata taccatattt atactttttc ttcaaactct atattatccc aatatttaat
     6241 gctataagat aatataaggt agatgaaatt tcatctacct ttagttttat aagttaatat
     6301 tgtattgttt atattttatc caaaacttat acaggtacta aatttcatct aagatgcctt
     6361 ttatatcact atctatatct tcatctagat aagaatctaa atcttctaag tcaacttcta
     6421 gttcttgata tgtcatatca acactgacat cctcatctat atccatttct gatattaatt
     6481 ctcctaaaat actatttcca tcagaatcta aaccactatc tatatcttca tctatatagt
     6541 tttcatttaa aatttcatct aaatcaatct cgtaataaac ttcattattt ttttcatccc
     6601 ctgagtccaa tcctaagaac tcatagttaa aagtccaaac attgtattta cttgacatac
     6661 tttattcctc ctgtgaaaaa catattttca tctagtttat tggttccata atcaacagag
     6721 ttatatattt tttcaattat gtcttttctt ctttcttttg tactattatt taatgtcaat
     6781 tgtttaaata catatgtatt tgttattctc tcgaataata tttcttttat actatcatca
     6841 tcaatatttc tatagttttt ccacaataac tcaaataact taaacatttc cctactagtt
     6901 tcatctacat aataaggata catattttct actaaatggt ttaatcttga tatggcttgt
     6961 tttaaaaata catcaagact tgcactttca ctttctttta acattctata attgtatttc
     7021 ttcttatctt ctcctgaaat tacaaagatg atattgtcta aacttttttc aacttcattt
     7081 aaataactgc ttatattata tgataagtca gacaatagat ttttaatctg cttaaacact
     7141 tgtacatcaa gtttagtatt atacttttca aacaaatcta tttcttgatt ttctaatatt
     7201 cttggcatat atgctgacaa taaatctaaa ttgtttcttt gttcttgtat ttgcttataa
     7261 agtgaattta taacttctat tcttatttca tcatttaact ctttttctaa aatcttatca
     7321 aattcagatt tcatatagta aatatttcca aaaaaattaa atattatcat atcattcata
     7381 gctttagacc aattcttata tatcacttca atagaatttt ctttaagtag aggttgcttt
     7441 gcatatataa atacatcttg cataagtttt tgaaccatat ttttacttaa gtttaaaagt
     7501 actttttgat tattaaattt tatttccgtt tctacaaaat tagaattttc gttttcaagt
     7561 tctttattaa ttacaatgtc ttttatatca ctatttttta tagatatgat ttctttttga
     7621 aaagacttta gaatagtcaa gctatcgaaa tcttcttgaa taaaaaatgg agcatgtttt
     7681 aatgtatatc ccactatttc attttctata tcttttaagt catctatttg cataatttct
     7741 ctatttttat catttatatg taggttaaat acatttttat tatcatataa tattatatct
     7801 tctcctaaat ttaacttaga acaattatct ttcgctgact ttacaatagt cttcttagat
     7861 ttcttattta taaaaattaa ttggtcttga tattttacta aaagcatgtt tgtgttttta
     7921 taatcaaatc catctatatt tcctgatatc ttgcagaata ctatatcaca atctttagga
     7981 agatttccta ttttttggtt atctttaact ctctcattta aatcagatgg aactacacta
     8041 tctatattgt tattaaatat agatacagac cttactataa tcccctctct ttccatatga
     8101 aagtatccct tcaatgacac tcttttatct tctaaaatgt atctttctat atcatcaaaa
     8161 tttatttcaa atctattttt cccactgata aatataaact tctcattatc tttattaatt
     8221 ccacccatat aaaacttatt gtcatctaaa cttaatatag tcatattatc ctttagatag
     8281 aatataaatt ctctttgact tttgcagatt aaatcatatt catccaaagt tattttcata
     8341 gtcaaatttg ctgtaacatt tttacttgaa ttttcacttt ttatgtaaaa aggcaagtta
     8401 tttccataga caattatttg agaatcttct aaaactgcct ctaatttttc tttattgtct
     8461 gaaaaactat atatatattt tacacctgta tcctcattaa cttcatctga tttgctgatt
     8521 attatattcc ctagatttgt tatatgaagc tttttatatt cttttgagta tctacttatg
     8581 ccactaagct caaacccatc agaaagctca tatatgattt tttctcctaa tttcaacata
     8641 ccttcaccac ctaaacagaa ttataagtat tttataaagg tgaataaaat gtctcaaaac
     8701 aaataatatt tttttaatac tttttattct gagacatagt ttctcctttt ttataatgct
     8761 ctaagcacct tcaactattt cttcataaag tttatctaaa tgtttaacat attcacgata
     8821 tccagatttg ttctttaact cacttcttaa atcttcatat ttttgtttaa gttcttcagc
     8881 actcaaagaa ggagctgatt cttttaattc ttctaactta taatcatagt acttatcata
     8941 catagctata tatgtatcaa tagatttctt agctagttca ttacattttt cttgtgcttc
     9001 tccatattga gaataatcac ctatttttat tttatagtat ccattttctt gaattattgt
     9061 tgaagaaata cctttagcct catattcttt tgctgtttct tttgctgttt tttcaacttt
     9121 aagtgaacct gcataaacag tgtaatatac acctttacca tctttatatg cttccatttc
     9181 ttttgctttc ttttcagctt gtgctttttc ttgagcctct ttttcagctt ttgccttttc
     9241 ttctgcttct tttttctctt tttcagcttg tgcttttttc tcttcctcag ttaatttatt
     9301 gttagcttgt tgttgagttg atgactttgg aatattctct ttatcatttt tcttattgtt
     9361 gcctactgtt ataaaatatc ctacaccaac aataaccact ataactactg ctataatagt
     9421 ttcttttaat ctactcttct tagaaacaac ctcttcatca tctaaagaat cttctttatc
     9481 tgatacataa tttttcattt ctttcttgtt ttctctatat ttgtccttag aagaattttt
     9541 agatttttta ttactatttt ctttcttttt aataggtatt actactgatt tttcattttc
     9601 taatttttct tcttttattt cttttgcttt tttatcttct aagtttttac tttctttttc
     9661 aagcttcgat tcattatttg aagatacttt ttcatctaag ttattattta ttggtttttc
     9721 tatttttaca ctacgttctt cttctatatg ttgacttggt tcttcttgtt ttatttcttt
     9781 taattcattt tgctctattt cttctgattt tgcttgatga ttttcaactg gtttatctaa
     9841 aattatatca ggagaaatat catttaaatt ctcttcttct tgttcaactt cttcattgtc
     9901 ttttttaata aatccaaata atttagcctt ctttttactc tttttatttt ctggttcaat
     9961 tatatcttct ttattgctgt cgtactcttt atctgtagta tctttatctt ctacaacgtc
    10021 acctatatct tgattattac tatctacttc ttctttagcc ccttcttcag catcatcttc
    10081 tattgaaata ctacttctaa agcaaatatc tttatctgca ttttttatca tgtaaatgta
    10141 ttcttcaata tcgatatctt tatttcttct agttcttttt gatgcaattt catgattttc
    10201 taattcacta tagttatctt ttattagatt ctgtgcttta ttccatgtat cttcactaat
    10261 ttctgtttta tttttaatta catagtccac aaattcctta aaattttcag gaccaaattc
    10321 tccaattata gtcagtctca ttgcaccttc tataaggtct ctatttcttc tctttaaaaa
    10381 ttctaataca accgttgcac aagcaaaatc tttaataatt tctttgccaa aaccatatcc
    10441 ttcaacagaa tgaagttctt ctaatccaca taacttctta tattcattgt gtattgtttg
    10501 agatatactt tcattattta agctatttaa cttatctata aacttcgatt ctatatcatt
    10561 tgattttgat tcatgtttta cttttgaatt tttaatttca tttagtagca tgtcattttc
    10621 tgaattatct ttacctaatt gcaaatcctc aatttcagtt gcttttattt ctactttgtc
    10681 ttgttcgtta ttctcttcca cattattagg taccacatct ttattgctag ctaatatttt
    10741 gtttacacag aatataattt ctctttttaa ttctcttttc gcatctgctt gatttttaag
    10801 aaataaatct ccacattctt tcttttctaa acttattttt ctaagagata tattaaggag
    10861 gtctttatat aggtttaaaa ccttataatc cttttctaaa cgtcttcttc tctctgatag
    10921 ttccatttta tcataagcta aaaaactatt taaatcattg gcttttagag agtctaatcc
    10981 ctctttcata gccttattta catactttct acattttgct gtagttaatt ctaaatcttt
    11041 ttctaattca tctatttcag gtatgtactt atctactatc catttgccaa taagtggttc
    11101 tgtatttctt gctaatgtct tataaaacaa tatattaaat tttaaaagct ctgcatattt
    11161 tgcttcatta aattctactt tttcaaattt cttttccaaa acaccatcta gcttttcatt
    11221 tattttagac tcttgtttta aaaaacttgc tgtgacattt aaaacagtgt ttcttattat
    11281 attattttca ctcaaaacct tcactcctta aatcaattat tttattgtaa tatttacatt
    11341 gtgcaaagtt gctaatttga ttatgatatc taaataatta tttactaaat ttgctacatc
    11401 ttatatcata tcatattatt tacatcattt tactttttct ttttatatct atcttatatt
    11461 ataccatttt taatatctgt actaacttat attacaatat tcctcaaaat tttaaaagta
    11521 gattagacaa atttacaaat aaaaaaacat cagtttaaac tgatgttttt gactatcgaa
    11581 ataattaagc aagtctataa gcggagttct gtattagata atcatctttc taggcctatt
    11641 gtcaccaata ggctcaagcg acctacctac cggagagtac gagcagcact ctttattcct
    11701 atcttggtct tgctccaggt ggggtttaca tagccaacta gtcgcctagc tgctggtgag
    11761 ctcttacctc gcctttccat ccttaccaca atagtggcgg tttatttctg ttgcactatc
    11821 cttaggatcg ctcccactag gcgttaccta gcaccctgct ctatggagct ccgactttcc
    11881 tcatgtaaga ttacatgcga ttatctggct tacttaatat ttattttact tagttatgtt
    11941 aacacaaatt cttttacatg tcaaagtaat attatactaa aaatttaaat ttattcaaaa
    12001 atagtgtaat atagatatat aaactccatt taactcgagg tgatatattg aaaaaattat
    12061 atagaatagt aattaacatt atactagtat tagtaatact ttacagtgga tttaatatat
    12121 actcaaaact taccaaatac aatcatgata ctaaaacatc ctcagaactt caaaaaaaag
    12181 aatataaaaa agaggattta tcaaagatta acagtgattt taaattttgg ctttctgttg
    12241 agaatacaaa tataaattat ccagttgtac aatctaaaga taattcttac tatcttgaca
    12301 aagattttta taaaaaagat tctatttctg gtacactgtt tatggattat agaaataaat
    12361 ctattgatga caaaaatatc ataatatatg gacataatat gaaaaataaa actatgttca
    12421 acaacctaaa taagtttaaa gatgctgact tctttaaaaa caataataaa ataaaaataa
    12481 ccttgaatgg aaaagaattt ttatatgatg ttttttctgc ttatattgta gaatctgatt
    12541 atgattatct aaaaacaaat tttaacaatg aatctgatta tcaaaattac ataaatgata
    12601 ttacttctaa atcattgtat aaatctccta ttaaggtaaa tagcaatgat aaaatagtta
    12661 cactttctac atgtacctat gaatttgatg atgccagaat ggtgattcat ggtagattga
    12721 tttaaaactc acagacacag tttaaaacaa taattttaaa ctgtgtcatc ttttatacct
    12781 ataatttata aattaggcca ctaaaagtat gtaatacaaa ataacaaata catcaaacaa
    12841 taaaaaacaa taaaaaaaga ctatgctaaa aacatagtct ttactggagc tggtgatagg
    12901 aatcgaacct acaacctgct gattacaagt cagctgctct accgttgagc cacaccagca
    12961 cacctattct attgtaaatt agtaaaacta atttttaaaa gtta
//
LOCUS       DAMJVX010000100        12955 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMJVX010000100 DAMJVX010000000
VERSION     DAMJVX010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12955
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.104"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(145..1452)
                     /gene="grdB"
                     /locus_tag="PC407_002457"
     CDS             complement(145..1452)
                     /gene="grdB"
                     /locus_tag="PC407_002457"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(403..405),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2497897.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVVTGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            complement(1538..2824)
                     /locus_tag="PC407_002458"
     CDS             complement(1538..2824)
                     /locus_tag="PC407_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2497898.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            complement(3103..4425)
                     /locus_tag="PC407_002459"
     CDS             complement(3103..4425)
                     /locus_tag="PC407_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2497899.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            complement(4425..6419)
                     /locus_tag="PC407_002460"
     CDS             complement(4425..6419)
                     /locus_tag="PC407_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2497900.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIKNTFSENTN
                     KTSEIEIYNKILKINTFPVEYKKNNTEYIVVLIKDITNEKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            complement(6506..7753)
                     /locus_tag="PC407_002461"
     CDS             complement(6506..7753)
                     /locus_tag="PC407_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2497901.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSSGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            7981..8526
                     /locus_tag="PC407_002462"
     CDS             7981..8526
                     /locus_tag="PC407_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2497902.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCIDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            8540..9226
                     /locus_tag="PC407_002463"
     CDS             8540..9226
                     /locus_tag="PC407_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDF2497903.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMIGNSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(9350..9970)
                     /locus_tag="PC407_002464"
     CDS             complement(9350..9970)
                     /locus_tag="PC407_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497904.1"
                     /translation="MNYNSNKNKYLLKDINLEDIFIDKNIVLKFLGYGNRKAPDTISE
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGIALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(9960..10982)
                     /locus_tag="PC407_002465"
     CDS             complement(9960..10982)
                     /locus_tag="PC407_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDF2497905.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEYIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRGYDNFLCDFGCNCLNTTPVENLQAAIKAGRINYKELDR
                     IDIDEL"
     gene            complement(10983..11621)
                     /locus_tag="PC407_002466"
     CDS             complement(10983..11621)
                     /locus_tag="PC407_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2497906.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            complement(11739..12890)
                     /locus_tag="PC407_002467"
     CDS             complement(11739..12890)
                     /locus_tag="PC407_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2497907.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDDDLDKMLELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TESLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
BASE COUNT         4163 a         2297 c         1301 g         5194 t
ORIGIN      
        1 tttatacttt aattggctgt tttactataa atatttttat tacttaattg ctttcttcat
       61 tccatccata gcaaaaagat atcataaaat aattttcaaa aactatttta tgatatcttt
      121 ttttatttct atattatatt tttattattc aaatactgtt tgttcttcta tatcctctgt
      181 tagtgcattt aaagctttta aaactatatt ctttctaatt tttttacttt cctcttctcc
      241 taacatagga tttccaagtg gatatggtat ccctactgct ggaacaattc tattagctcc
      301 tattgtaagt gatattggta ctactgtaca tatatgaaca actgctatac cagctctctc
      361 aatctctttt accatcgttg ctccacaacg agtacaagta cctcatgtag aagttaaaat
      421 tacagcatct acatcatctt ctaacaattt cttagaaaac tcttctccaa atctcttaga
      481 attgccaaca ctcgttcctg ttcctgtagt agttataaaa taatctacta attctccaat
      541 tacaccttct ttttcaagct ctctaactac atctaatggt acaactaaat ttggattttc
      601 aaggacatat gctctgtcat accccccatg tatagttgta aattctttat ttgacataga
      661 atccatatct gttaaatcgt aaattccata tttagtagca cttgaagatt caattttgtc
      721 tggattatca attggaacta tccctccaga agttactagg gcaagtttta tcttagataa
      781 atctttaaca ggtttcatag gtgtaactct atcaaattca ggcattggat actcagtttc
      841 aaagctttcc cctttcatct ttttaaccaa catatcaatt gctctttcag accctctaag
      901 ttcatggaag aaattttttc ttattcctct cattatatat ccttcctctt caggaggtcc
      961 tatttcctca cctctaacca atttaagacc tagtttcgtc attattggaa ctacctttct
     1021 aagacttgct gctgaatttc ctgtagacac tatatgcaaa tctaatttaa acatatctac
     1081 acctggattt tctttataca ttcctgtaac tacaggaata tttaactctt cttcaactgc
     1141 cttacaaata gtcccacaag ctactccata tctacctgca ttaaatgctg ggcctgcaat
     1201 aaataaatcg gggctttctt cttttatcat ttttaaaagt tcttgagttg cactctcaat
     1261 attttcacca aaatacgtat ctccacaaat tacagttgag attatctctg catcatcatt
     1321 aaattgactc tgaaatgcta ttccaggtcc tactacacca gccctttttt ctggcggaat
     1381 atctgctttt tcctctccac ctataccagc aaagaaattg tttatataat gaaccactct
     1441 gattttactc atctcttaat cctccaaatc actttatagt tattacatca attagaccaa
     1501 cgattacatc aacagaactt tatattcaaa tacttattta ataatatctt gctgtcaaat
     1561 gattatagcc atttgctata gttgatgcta ttataatttg tagctctgca tccatgcttc
     1621 cgtcttcatt taagcatcct ttatacccac ctatcatcat ttcaacataa tctaaagttc
     1681 ctataatttt ttccattgct gggaaatgaa ccactagatt tccttgacca caagaaacaa
     1741 ctgcatctgc ttccttagta gcgtctgcta tagattgact cttgccatct tttccaggaa
     1801 attcatccgt tatcaaaact acatttgctc caacttcact acattttcta caattcatca
     1861 ttaggtcagt atctggattc ccataacctt cttctgtcac tagaactcca tcaactccta
     1921 aaaattctat taatttagct accatatcag agcttctctc tttatcgact aagaatacgt
     1981 tctcattagt caaaataact cctataaagt ttaaatcttt tccatgtctt ttatataagt
     2041 catgtataac tggattattt aaatgatgga atgttgtaac cttatcacat ggtgctacac
     2101 agtttccaga tattatagct ccatccatta tttcagttgg atacataaat gtaggcacta
     2161 tctgtttagc atccacccca tagtaataag tgtcatgtaa aagtccttgt gattgtaaca
     2221 tgtgaacata tgctactgtt ggcaggtctg gatattcttt tacttgtttt aataatggct
     2281 ttgtctcata tacaatgact tcatctggct caacttctct tcctgcctca cctacatatg
     2341 cacctatttt taatcctgca attctagctg cttcttcata atcatgagta tctataccat
     2401 cctttgcttt tataacaata catatattat ttgtttttga aaaaggtgta tatttagcag
     2461 taggaccact catatcaatt acgccttctt gaaatccaac tatatttcca caagttacaa
     2521 cacaagctcc taaaagtaca tgggttcttc cacttcctac agtcttcact ttatttataa
     2581 ttccaggaaa tattttgctt tcatctccaa ttttaactct aggctctatt acatctttaa
     2641 ctggagctat tcttattgat tcgccaggtc ttgcaagttc aacatttaca cttacaatcc
     2701 tttcatcttc taatactatc ttttctattt cttcgctact aatatatagt actccatctt
     2761 ttacttctgt cttttctcca aattcaacat tttttataaa tatattccct agctctagct
     2821 tcatatgaaa atccccctta atatatttaa tcgcaattaa aaatatttat tacgtttatt
     2881 aatatataaa gcattttcta tgccaattat ttaaaatata tattctatgt attaatgtca
     2941 tagaaatttt catttttaat ttacaccagt attgaattta ccaataatta ttgtaaatat
     3001 taatttttaa aattataata aaaaaagata aacagatttt ttctctttat cttttaatac
     3061 agtttgagaa atttttttct cacttaatga gaagtttttt tctcacttaa tattatactc
     3121 tgatatttta ttggttaact gtcttctact aatctccaat tttttagctg tatttgaaat
     3181 attatttcca cacaaagata aaactttttc aatatactca cattcaaact cttttcttat
     3241 ttctcttaaa ggtctaatac ttattttatc atctatgtat acattatttg atattaaatt
     3301 taaattgtct tttgataaat tgccttcttc acttaaaact acaagtctat ttattatatt
     3361 ttttaattct cttatatttc ctggataatt gtaatttaat aaaaaatcct ttacttcctt
     3421 atcaatcgag tgtatctttt tatcatgttc aatttggtac ttatttaaga aaaattctat
     3481 aagagtagtt aagtcttctc ttctctttct tagtggtggt atagttattg ttattgtact
     3541 tattctataa aaaaaatctt ctcttatatt tccatttgat atttccactt ttggttcttt
     3601 attcatagca caaattaacc taaagtcaac tttaatagat ttattacttc ctatcctctc
     3661 tatagatttg tcctcaagtg tcctcaaaag ttttacctga acatttagag gtatatctcc
     3721 tatttcatcc aaaaatagag ttcctttatt cgaaagttca aatctacctt ttctactatc
     3781 tactgctcct gtaaaagaac ctttttcatg accaaacaac tcagactcca atagattttc
     3841 tgaaaaagaa caacaattta caggtacaaa tatctcattc tttcttggac ttatactatg
     3901 tatatatcta gctaatatat cttttcctac accagattcc cccaagatta atatattaac
     3961 atctttacaa gcagcttttt ttgctatttt tatgatatta ttaaaatctc tatttttaga
     4021 ttccaaagta aattcaaaat tatttttttg tacagaaaca gatattttac ttgcctttac
     4081 cttttcaact tcttctaata aattttcaat gggattactt tttataaaat aagataaagc
     4141 tcctcttttc atagcatcaa ctgcattttc aatacttcca tatcctgtaa ctaatataac
     4201 ctctatatct ctattaatag atttaattct atctaatagc tgtactccat ccatattttt
     4261 catcataaca tcagataaaa ccaaatcata atttttatct tttttaatta tatttatagc
     4321 ttcttcacca cttagtgcta tatctacaag atagcccttc ttttgtagaa tttttttcat
     4381 taccactcca tattctagct catcatcaac taatagtatt ttcattatat agtcacctct
     4441 ttatttaatg gaatttttat atgaaaacaa gttccaaccc ccaatttact ttcaacactt
     4501 atatctccat tattcttttt aatctcatta taagtaatat ataaacctaa accagttccc
     4561 tcaccaattg gttttgttgt atagaaggga ttaaatatgt cctttagggc atcttctttt
     4621 attccttcac cattatcttt aaaatctata aataaacaat gatttttttc ataacagtat
     4681 ataataattt ttccatcttg atgtattgca tctattgaat tagaaattaa atttataaat
     4741 acatgtttta acgattctcc atttatatag catactaaat tatgttcaca gtcaattttt
     4801 atttcaacat tctttaactg taacatttta tattgaagtt tcactatatt ttcaataaaa
     4861 tttcttatat ttatatgttc taaattatca tctgatattc ttgcgaaatt taacaagttg
     4921 gttattatat tacttgccct atctacatta ctttctattg atttaacaca atcattcact
     4981 tcttccattg taacgttatc ttttaataga tagcaattat ttcttattat acccaaagga
     5041 tttcttatct catgtgctac cccagaagct aattgtccta tagatatcat tttgttttct
     5101 ctaagtaatt tcgcctctac tattttttca ttagttatat ctttaataag aactactata
     5161 tactcagtat tatttttttt atactctaca ggaaaagtgt ttatctttaa aattttattg
     5221 tatatttcta tttcactagt tttattagta ttttcagaga aggtattttt aataacacct
     5281 tttatcatgc taatcaaatc tgctatctta taactcattt cacccataaa gtcatatagt
     5341 actttgttcg attctactat attgttattt tcatctacaa gcattattat atctgtaata
     5401 ctattaaaag ttgcctctaa agttttttta ttttctatta cctgttctgt tttccttttg
     5461 atttctatct ttaacaaata tgtatagcta taaaaaacat aaattgatat taaaattata
     5521 cctataaaaa gataaatact aggcacaatt cctcgctcat acaatatatc atcaacttca
     5581 ttatacgtag aataccattt tttctttaaa tctttataca ctccattttt ctgaagttta
     5641 aaaatgcctt tatttaaaat actaaccagc tcttcatatt gtttaggtac agcaagaact
     5701 gcttttttag tatatatagg attacttaaa acactgtatt tatttgaaag tccataatta
     5761 tttatatagt atcgcaatac tggttcatca ccaacaactg cgtctacttt tccagacatt
     5821 aaatggttga ctccagtctt tatatctgga gtcaataaaa tgtttatatc ttgaattttt
     5881 tgctttaaaa aatttataga ataatcacct tctggaattg ctactgtttt cccctttaaa
     5941 tccatgtaac tatttatatc ttgggacttt ttatctttca atatatttgc actcaaagtg
     6001 tatattgggt cagtaaatat aaatttattt gccctttctt ttgatggaat caagtcaaaa
     6061 aactttatac tatcatctat tgaatttacg aaagattctt tccaccaatt atttggttta
     6121 aaataaaaat ctctaccaat ttgaattgac agagaattta ttaaatcaac aataattccc
     6181 ttatactgtc catcttcctt acccttatat ctcaaaggag gtgaactttg gtcagagctg
     6241 taaatcaaat tcttgtgttt ttctaaccac tttttttctt cttctgtaaa tggaagtgac
     6301 ttcttaatat attcaaatac atttaaatta tactccatat ttacatacat atttgtaagt
     6361 gaaaatacta ctaaaaatga ataaataatc cctataataa caatcttttt tttattcact
     6421 ttaaaactcc aatcttcttt tttactataa taaattcaat atttaataac aaaatcctac
     6481 ttttttcatt aaatatttat atttattata aaactaattg aatagatgtt gaaattatag
     6541 tcattactat tcctatcaat gcttcatatg gaattaactt aaatctttca cctatatcca
     6601 tgtttatact acctgaacta gtatgaaata gtgagccatg tggtaattgc tcgaatacac
     6661 tagacccagc atttactatt gatgcacctg aaatcggtga tagcccacca tttattatag
     6721 catcatgaaa agtagatgat gctatagtag caccagctgt tgatgatgct gttgctaatg
     6781 acataagtat acccgaaact ggtgccaata aaaattgtgg catatttaaa aattgtaaag
     6841 cacctattgt actctgttgt aattcagaca tctgtataat tcctgcgata gtccctgttc
     6901 caagtaataa tatgcatacc ccttgcatct tagataatcc aaaagctaaa tattctcttg
     6961 aattgttcaa gtttcctgtg acaattagag ttacgattcc tcctattgga agggctatca
     7021 ttgggtctat aactatagga gatacatttc ctaaaaacaa tagaaatata gctataattg
     7081 gaccacataa tgatttaaat aaacttggta aatcttctct ttgctctaaa atttcatatg
     7141 attgtacttt atttccttta tttattaata tacttgctaa aataactgta ataacaactc
     7201 caataattgc tggaattata tttgccatca taacactaga gagattgaca gaaaaattat
     7261 ctgctactgc tatagtattt ggatttggtg atattatatt acctgcttta ccccctccca
     7321 acatagctaa tagtattgac aatttagaat aatttaattt tctacctata tataatccta
     7381 taggtgctac agttattata gatacatcca aatttacgcc tactccagca agtaccatag
     7441 ttgaaatggc aatcgaaaat aaagccctct tttcaccaaa tatttttaca atctgttctg
     7501 atattttatc tactgctcct gtttttatta aacttccagc taatacacct gatgccaaag
     7561 ctctaagaat tgatggtgat atattcatag ctccatttgc cataaaatct acagtttgtg
     7621 ttatatttgc tcctccaact aatcctccaa taaatgctcc taacattaaa ctatatacag
     7681 cttgaaattt ttttataatt aaaataattg ctattaataa accaaatata gctccaaaag
     7741 tcgttatttg cataatgtcc cctcctatca taaattaact atcttttgct taattttatt
     7801 taaatatatt ttaagtattc tgtatccact ttgtcaatta tattttaatt tatttaatta
     7861 tattgaacat agttttttca aaacctaatt ctatatgtta taataatttc atactacata
     7921 atcattttac ttgattattt taagttaata ttttttaaat taaaaagaga ggatatttat
     7981 atgaatgatt taaatttagg ggaaaaaata gctgaagttc gaaaaaaaca aaacttaagt
     8041 attcgagatt tagcaaaact agctgatgtt acaccatcat tattgagcca aatagaacga
     8101 ggtcttgcta atccatctgt aaattcatta aaatctatag cctcttctct aaacgttcct
     8161 ttatttacat tttttgtatc tgaagtagat aaaaaaaact tgatagtaag acatgataat
     8221 agaaaaaaag ttattttacc tggaagcaaa gaagttatat atgagatttt aattccagac
     8281 tctagtggta atttagaatt tgcaattatg gatttagctc caaatacttc ttcttgtatt
     8341 gataggatta ctcataatgg agatgaaatt gcatatgttt tggagggaga agttaaactt
     8401 tttatggatg atgatgaatt tactttgtcc aaaggagata gtgtaaaagt tcctttagga
     8461 acaaagcata aatggcaaaa tgactcaaat agtgaaagta aagtaatttt tgcagtcatc
     8521 ttgtaaagga gtaaaaacca tgaagacata taaatttata ttttttgatg cagatgatac
     8581 tttatttgat tttaagaaaa gtgaatcaca tgcttttaaa aaacttctat ctgagtttga
     8641 tttagaattt aattttgaga attatataga gtcatataga aatataagtg ataagctttg
     8701 gctcgacctt gaaaaaaata ttataacttt gaatgaactt aaattactta gatttgaact
     8761 ttttgcaaat aaaatatctt tggatgttga ttctgaaact cttagcaaaa tgtacttaaa
     8821 ttttttaggt gaatgtacat ttttaattcc aggtgcaatc gatatattac aatatcttaa
     8881 aaaaaagtat actattgtta taataacaaa tggaatttct gcagttcaaa agaaaagact
     8941 tgaaaattca aaaataaaag gatatattga tggaatggtt gtatctgaag agttgaaaat
     9001 atcaaaacca aatcctgaaa tatttaaata cgctctcaaa aagtttaatt gtcatgataa
     9061 atcttctgcc ttaatgattg ggaatagctt aacctctgat gtcttaggtg gaataaattc
     9121 aggaatagat acttgttggt taaattccaa taacagtata aattatacta atcatatacc
     9181 aacttatgaa ataaatacac ttattgaatt aaagaaacta ctatagtttg taagtctgtt
     9241 caaattaaat agaatattac tttgcaataa tttttttagt ttatacataa aatgaccaaa
     9301 aaaattattg caattcatac acaaaacaaa tcttagtctt gaaaactagt taaaagcatt
     9361 ttctaggaca tttgctacat cttgattcta aattttcttc atctcccatt aaaataagca
     9421 ttgcaacact ttttatgggg gatagttgat gataatcatt gatttctatt tctatactat
     9481 tatatttatt ttttacatag ttataaatat ccttttgtct ctctacttca aaatctttat
     9541 ctcctggata tatttcatgc gatattttaa gactatcata ctttcctttt aaatacattt
     9601 ttattttctc attaatataa tctaaacata ctatacctat tttatccaat gctatccctg
     9661 ccatcatatt gcaattttcc atatagtcat tcatcttaat atcaatttta tcccctattg
     9721 tatatagtac agcaaatgct tttttacatt ctttaggtgt tttattaata tcaacttcac
     9781 ttatatatat cttgatatct aaaatatcat ttatatttct tatttctctt tcaacgattt
     9841 cactaattgt atctggagct tttctatttc cataccccaa aaactttagc acaatattct
     9901 tatctataaa aatatcctct aaatttatat cctttaaaag atatttattt ttattactat
     9961 tataattcat caatatcaat cctatctaat tctttataat taattcttcc tgcttttata
    10021 gctgcttgta aattttctac tggagtggta tttaagcagt tacagccaaa atcacataaa
    10081 aagttatcat atcctctcat cttctttaac aattttagtg tctctcttct aatcttatct
    10141 ggcttagaac gtccaagaag ttttattggg tctaaattac caatcaacac tatatctcta
    10201 ggaatcattg gagcaatttc ttcatagtct aaaatttggt ctaaacttac tgcatctata
    10261 ttacaactta gcatattatc taataattct ctggtgtctc cacatatatg catacctttc
    10321 caacaatcaa gttcattgta gattttattt aaatttttta ctatatattc atcaaatcta
    10381 tctttactta gggttactga cgttggctct gctattgaaa tatattttgc tcctgcttta
    10441 tttgcagcta cagcatatct tcttaccaat tcagttgtaa attccaacaa ttcttctaca
    10501 aattttttat cagttattat acatcttaaa agttgagtag ctccagctaa ttgacctgca
    10561 agtgtaaatg gaccttgtat cgattcatat agtggttttt taatactttt tgatataact
    10621 gataagctct ctatattgag tggcatacgt ccactcgtat aagggtctgg aacatctaat
    10681 tttcttaatt tatctctgtc agttatagta tgtgtcaata cacttggaaa atcataatct
    10741 ggtcttaatg tctctaaacc cagagtttca caaaatgtaa tcccatcaca gaatgagtat
    10801 ataaaatctg attcaaaata cttgtccata gtctttgcta attctaattg caaatctaca
    10861 ctatcatata cttcatatgc ctttttattt attaagaaca atccatttgt acccatatca
    10921 ggcagaaaaa aacctctatc ttcactattt atatactgaa gtaaagtcat atctgaaaac
    10981 atttaaactt cacctcctat aagtttgttt attaatttaa ctgcttttgg agcatttgaa
    11041 ctgtatccat ctgctcctat ttcttctgca aactccatac ttacaggtcc tccaccaatt
    11101 attacttttg gtcttttatc tatctcaatc atattaagct cgtctataac cgatttcata
    11161 ttttccattg tagttgtcat cattgaagat aagcctatta tatctacatt gttttttata
    11221 gcttcttcta ttatatcttc actttttcta tttacaccta agtcaataac cttatatcca
    11281 gtggcttcta ccattacttt tacaatattt tttcctatct catgagtatc tccttctact
    11341 acagacatca gaattactcc ctttgatttt ttctttactt ttccagttcc cttcaaaaga
    11401 ttaatacctt tatttaatgc atctgtgcat actataactt ctgaaagata gtattcctta
    11461 ttctcaaact tatctaaagc tcctatcata cctttgttta aaccatattc ataaatatct
    11521 tccacatcaa tatttttatc tatagcaatt tttactaaat tctctatatt atctacaccc
    11581 atatttaata tacattctgc tatatcatca agtatgtcca tataatactc atcctttcta
    11641 attacttaca aaattgcttt ttcaatatcc aataatttct cttatttaac aaggactaac
    11701 tctaggtatc aatcaatttt atttaatatc atctaaaact attgataatt taaatttgca
    11761 gtccatggaa aatctacagg aaatgccttt gtagcaaatt catatatatt ttttagatgt
    11821 ttaaattcat tatactgttc ctcatttttt tcaaaaaaca gtttattaag cttatccaaa
    11881 ttttctatta caagttcttc ttctttgtat ttaataatat atttattttc aacttgttta
    11941 aattccatgt attttagtaa ttcaaaatct acatattgac taaaataact tcttaaacta
    12001 tcacacaact tagtaaaatt tattattttc atagttcctt gttgatagtc caaagattct
    12061 gtcccattac atttcaactg gtctttaaga tttttcacat gtactttata atttaaatat
    12121 tctaatccaa atttatttgc aacatatttc aatatatttt caacatttac agagtttaac
    12181 cctatttcag caacttctcc tacagtagaa tcttcttttt ttattgttct tattatcaaa
    12241 tatcctatta tattattatt ttcctttata aataccttct tgtaaccaat tggtccccag
    12301 gcaatagttg ctgcatgcaa tagtttttga aactcatctc tcgttcttat aaatctagta
    12361 ctattttgat tatatagttc aagcatttta tctaaatcat catctttttc aaaattagtt
    12421 tcatcaaatt caactatttc atatgctatt tttacatctt caggttttat tgtatattta
    12481 taaaaactct ttaccaaact acaatttctt ctactgtata gacttcttgt tcctgatata
    12541 attacaatat ctacaccatc ataaaacatt ttttcttcta ctttatccaa tatcttagat
    12601 gaatagcctc tcttttcata atctggatga gtacatactc cacctatagc tgcaaccttc
    12661 aatctatttc cttgaataga tacatcttga attagatagt ttacatcaga aaccacttta
    12721 tcatcttctg atattataat catattttct atattatttt tacttagtaa aagaggaaat
    12781 tcttccatca tagttggctt atgatttctt gatattctaa atacatagtt tattaattct
    12841 attactttat caaattcttc taattttgca cttcttggtc ttaattccat tcatattcct
    12901 ccaaatatta tttaagatac ttaaaactct attatctata ataaaacatt tatta
//
LOCUS       DAMJVX010000101        12895 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMJVX010000101 DAMJVX010000000
VERSION     DAMJVX010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12895
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.105"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            134..1993
                     /locus_tag="PC407_002468"
     CDS             134..1993
                     /locus_tag="PC407_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2497908.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVC
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICINKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEEILDKEFVYNS"
     gene            complement(2285..2884)
                     /locus_tag="PC407_002469"
     CDS             complement(2285..2884)
                     /locus_tag="PC407_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2497909.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(2987..3784)
                     /locus_tag="PC407_002470"
     CDS             complement(2987..3784)
                     /locus_tag="PC407_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2497910.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3897..4002)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4094..5080)
                     /locus_tag="PC407_002471"
     CDS             complement(4094..5080)
                     /locus_tag="PC407_002471"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2497911.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLNEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5564..7294
                     /locus_tag="PC407_002472"
     CDS             5564..7294
                     /locus_tag="PC407_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDF2497912.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7404..7484
                     /locus_tag="PC407_002473"
     tRNA            7404..7484
                     /locus_tag="PC407_002473"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7438..7440,aa:Leu,seq:tag)
     gene            7716..7889
                     /locus_tag="PC407_002474"
                     /pseudo
     CDS             7716..7889
                     /locus_tag="PC407_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
     regulatory      8381..8485
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            8695..11169
                     /gene="gcvPA"
                     /locus_tag="PC407_002475"
     CDS             8695..11169
                     /gene="gcvPA"
                     /locus_tag="PC407_002475"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDF2497913.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHHETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11169..12626
                     /gene="gcvPB"
                     /locus_tag="PC407_002476"
     CDS             11169..12626
                     /gene="gcvPB"
                     /locus_tag="PC407_002476"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDF2497914.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPLIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         4834 a         1536 c         2133 g         4392 t
ORIGIN      
        1 aactatataa aacaaggttt atatattcta atagtataga aaattttatt gtaaacgcat
       61 gtatattatt agaaaaaata gtttgaaggt attcaataaa cataaaaaac aatatagggg
      121 ataaaggtgt tttatgaaga gatttttgag gagaattatc ttagttttat ttattttgtt
      181 gatttttata agttttattt ctattaaatt aattcataat gttggagatt atggaaaatt
      241 gattaattat gttgggattg tttgtggagc tagccaacgt ctaactaaac tagagatgaa
      301 tcatcaacca aatgatgaat taatagaata tattgatgaa atcctacagg agttgattac
      361 agggcatgga gattatggtt tagtaatcac tgattgtaat gaatacaatg aggacttact
      421 tttattagaa aaaaagtggg aagatttaaa tattgaaatt aaaaaagtaa gaatgaaaga
      481 acagaataac cagttattaa gtataagtga agagtttttt tctcttgcaa atgatacagt
      541 gtttaaaata gaaaactttt ctaaagaaaa aagtaattac ttaatgactt taataataat
      601 tatatctatt ataggtatat tagcatgtat catattgatt ttacaatata gtaaaaaaat
      661 ggttagactg gaaaaattaa atgtagattt aaaaaatatt gcatataaag atgaactaac
      721 aggagtaaat actatagaaa aatttaaatt ggatgcaaat caaaatattt gtataaataa
      781 ggataagaaa tttgctgtat tctatattga ttttgaaaac tttaaataca ttaatgatat
      841 atttggatat gattatggtg acatgatact gaaaagatat gctaatctta tgatgaatga
      901 catagggaaa tatgaaatat ttgcaagaga aattgcagat agatttgttg cgttaagatg
      961 ctatgtagat aaggaagatt tagtagtaag acaacgagat gtagacaatg aactaattaa
     1021 tataacaaat gaaattaaaa ataaacatag tattactgtt gtcagtggaa tttgttgcat
     1081 tgaagatgta aatgaaaaat taagtataga tggtttaata aatagagcaa attttgcaca
     1141 aaaaactgtt aagaataaac caggtactaa ttatgctttt tataatgaca gtattcgtaa
     1201 aaaaatgatt gaagaaaata ctatcaagag tagaatacat gaagctattg aaaaaagaga
     1261 atttatagtt tatttacagc ctaaagttaa tttacatagt caaaaaataa attgtgcaga
     1321 agctttagtt cgttggctta ctccggataa aggtattata tctccagcaa tatttattcc
     1381 agtacttgag aagaattttt ttattgcctt agtagataag tatgtttttg aggaggtttg
     1441 taaatggatt agaaagaggt tagatgaaaa taaacctttt gttcagatat cagttaatgt
     1501 ttcaagaata caattttata acactaaatt tgtagaaact tattccaata tacaaaataa
     1561 atacaggata cctaaaaata ctatagaaat tgaatttaca gagagtgttg cttttgagaa
     1621 tcaaaaacat cttttggaaa ttatacatga tttgcatgaa aatggattta cttgttcttt
     1681 agatgatttt ggaaaaggtt attcgtcttt aagtgtttta aaagatttac catttgatgt
     1741 tttaaagctt gactctatgt ttttcaaggc tagtttagat aaagataaag agaaaatagt
     1801 aataaaaaat attgtacata tgttaaaaga gcttaatatc acaactgttg ctgagggcat
     1861 tgaatgcaag gaacaagttg aatttcttag ggatattggg tgtgatttag ttcagggatt
     1921 tgtattttat aaaccaatgc caatattaga atttgaagaa atattggata aagagtttgt
     1981 ttataatagc taaaatgtac tttataaaat atgaactaat taagtaaaat tattttgtac
     2041 tgaataaatt tttactttat ttacagaata gtgattattt acagaaaagg gcgaaaagtg
     2101 tattccttta agtatgggat ggatagcaca aataggggag aaaaaatatt ggccatataa
     2161 gaatgcataa taatataaat atttgaaagg tcagcattat ataaaaatta aaaagaaaat
     2221 aaataatttt tctataaaaa agagactatg agaaaagtta ctcatagtct cctaaataat
     2281 gtatttattt tcttcctgca acccatttta agccccaatg aaattcagta tccatttctc
     2341 tatgaccttt gaagtattta actacttttt caacactgaa tgtctcatca tttacatttt
     2401 ctatcaatgc tattgaacac atattatagc catttttatg ttcatcaagt ttgacaacta
     2461 agtctgaatc ttgtttttgt ttgattgtta caattccatc agcttcagac cagttagcta
     2521 caccttcgta tataaaagca taaataagta ttctttttat atcttttatt ttattaccat
     2581 ttattcttaa attttctcct tgtgtattac ttcctgttct gtcatctcca tctaattgag
     2641 caaaaggtgg attgtctaaa ctaccaaaag catttcctaa tgcttgaaca gcacctttta
     2701 caccattttt catctcaaat aggcaaccca aatctaaatc tatattactg cttcttaaag
     2761 agccaaaaaa accctttttt tgtacttttt gattccaatt taaattaact aagatttcac
     2821 ctaaatcgct attttccttt tttgtaaggt ttattttttg tccctttttt aattctatag
     2881 ccatagtaat cttcctccac aaacatagat ttatttaaat ggtagcactt aataagtcaa
     2941 tattcaacaa gattagaata ataatattaa aactatacta aatatactaa aaagtaggaa
     3001 tcacaatacc tttgtatttt tcttcaatta agcttcttgc tttatctgat gtaagaacat
     3061 cttgtagaac tttaatcttt tcactgtctt tatcttcagt tctaaatgct aaaagatttg
     3121 catactcgtc atttatagga gctgtataaa ttccgttttt cttggcatca agaccagcag
     3181 gtacagcata agtagtatct ataaatgcag cagtaacatc tggaagagaa gatggaattt
     3241 gagcaatatc taactctttg aattgtattc cctttggatt ttctattatg tccttaggac
     3301 taactaatcc atccccttta ggtaacgtta aaagtccaat actttcaagg tatctaaggt
     3361 tcttagagca agaagatggg ttattgctta ttgcaattgt atctcctttt ttaaattcat
     3421 caacagattt tatcttataa gagtaaagta ttgctgggca tacatatagt tttttaccag
     3481 ccattatttt gtaacctttg gccttaacag cttcttttag ataaggttca tgttgaaata
     3541 gatttgcatc aatttcacca ttatttaaag cttcgtttgg aagtatgtaa tcatcaaagt
     3601 ttttaacttc taaagtatat cctttttctt taataagtgg ttttaattct tctaataact
     3661 ctcctcctgg aactaaagta gcaccaacaa caattttttt atcatcttta gctttagaac
     3721 atccaaccac agctaaagtt agtactagac ataataacaa acataataat tttttgaatt
     3781 tcatataaat tccctcctga atataattga ttagttaaaa ataaaatacc attctttatc
     3841 acactgcaaa ataaaaataa gcctgagttt aaactcaggc tttaaaagct aatttagact
     3901 catctttcag tataatactg caggatttag caccaatgta tataaaaata tactggttgc
     3961 tgggtttcat agggccagtc ccttcaccac tctggataag aagtatcaat ttttaattat
     4021 gttataaata ttatcatgat tttttacgtc tgtaaacata attttataaa tattttaaaa
     4081 tctttaatgt attttattta acacctaaaa ttccagaaac tagaatatca gtatcagcac
     4141 ctaagaaata gtcatttatt tcaatagaat ccagagtatt tctcaaagca ttttcttcat
     4201 gccttatacc aataagttta ttttctacaa atgcaacatc acattttcca aaaaaatctc
     4261 caaaaaattt tatactagtt attatgcctt tatcaacatt taaattaaac tcaacatttc
     4321 caccagaata ttttaattca tttgaaagag tatattttgg tgaattacca aaattccaat
     4381 cccaagtttc gtattttgat ttagtaagct tttctatttg ttttatatca tgctcattca
     4441 aacaataatt agtattatca gtatttgttt tagaaagata atccattata gcatctttaa
     4501 attctaatat gtccattttt gaatgtaaat gttcacttat attggttaca cgagatttta
     4561 cagattttat tccttttcct tcaaatttta taggtttaac ctttaatgct tttgagaggt
     4621 catttatttc agatgaaaat aaaagagtac catgatgcat tactttgttt ttgtagttgt
     4681 attgagcatt tccagaaaat ttcttgccat ctataagaag gtcatttctt ccagaaaatt
     4741 cggcattgac atctagtgtt ttaagtaaat ctattatggg ctgagtgaat cttctgaaat
     4801 cactaaaatt attattgtta caagctataa atgtaaaatt tatatttccc aaatcatgaa
     4861 atacagctcc accaccagac tgtctcctaa cgacaggtat agaatattct tttatatatt
     4921 ctaaatttat ttcagaaagt gtgttttggt tttttccaac aattatagaa ggttcattcc
     4981 tccataaaat aaataagtct tcatcaaaat tttttagaag atattcttcc attgctaaat
     5041 taaaataagg gtttgttttt tcattataaa ttaataacat taaagtagtt cctccataga
     5101 attgataaaa tttttattat gaatacaatt aattttataa ttcgacaata atataaaaac
     5161 tcctttaaat tagaaatttt aaaaactacc ttgcaaagtt taaataaata atatatactc
     5221 aaaatatgtt taataaaagt gaatagaagg gtagaggcgc aataatctat cagtactaat
     5281 aaggagttaa gcacagagag ttattaggaa aggagatatt gccgaagttt tataatcgat
     5341 gctttaagat tatattgctg ggggtacata gaaaatatgt acaactgtca caggtttatt
     5401 tgtggtgagc tatcatttaa ggaattatat tatatttatt tttactattg ttgatatatt
     5461 gtaatgttta agccttgagt gtttaccact caaggctttt ttagttttat tgtaacataa
     5521 taccctgtac agttcacaaa attaaaagga gagggagatt tatatgagga acaaaaaaat
     5581 taatattatt tttctgacga caatcatgtt cataatgtca acagtaatgg tttttgcaga
     5641 agaagatata gacactattg cactagctaa tgcagaaaaa tttgggattt taactttaat
     5701 accaccatta gttgctataa tacttgcatt tataactaaa aacgtaataa tttctttact
     5761 tataggtatt ctttctggta gttttataat taaagctagt ggtattaatg tttttgcgac
     5821 atttatacag gcatttttag atttagtaga tagagcttta gtatctttag cagacccttg
     5881 gaatgcaggg ataatactac aggttttggc aattggagga gtaataaatc ttgttgctaa
     5941 aatgggagga gctaaagcta tagctgaagc acttgctaaa agagcaaagt cagcaaaagg
     6001 gacacagctt ataacatggt ttttggggct tttagtattc tttgatgatt atgccaattc
     6061 actaatagta ggtcctatga tgagacctgt tgcagataaa atgaaaatat caagagaaaa
     6121 gctagcattt ataatagatg ccacagcagc accagttgct ggtttagcta taatatctac
     6181 atggataggt cttgaggttg gtttaataca tgatgctttt gaaagtataa gtatagatgt
     6241 agatgcattt ggtatatttt taaatacaat accatttaga ttttacaata tattaatatt
     6301 agctttcatt gtaatatcag cattattatt aaaagaattt ggtccaatga gaaaagctga
     6361 gataaaatct agaagtagaa aaattagtat agaccttgat gaaggcgtag aagagttgga
     6421 tgatttagct ccaaaaaatg gagtaaaatt gagtgtatgg aatgctataa tcccaatagg
     6481 aacattgata atagttgcat tagcatcatt ttattatagt ggatatacat ctataatggg
     6541 aggagacgat aaggctctga tacaactatt tacaaattct ccatattctt ttgaagcaat
     6601 aaaagaagca tttagtgcat ctgatgcatc tagagcatta tttcagtctg cacttgttgc
     6661 aagtttagtt gctattataa tggctgtagt aaaaaagatt tttacaatat cagaagcaat
     6721 tgatgtttgg atagatggaa tgaaaagttt agtaatcact ggtgtaatat taatacttgc
     6781 atggtcatta agttcagtaa taaaagagtt aggtacagct aagtttttaa tacacctatt
     6841 atcaggttca ctaccaccat tcttattacc aagtttaata tttggtttag gagcaataat
     6901 atcttttgct actggaacag cttatggaac aatgggtata cttatgccac ttgcaattcc
     6961 attagcatac tcattaaatc ctgatatgtc ttatgtaata gtaagtacta gtgcagtttt
     7021 aacaggtgca atatttggag accattgttc tccaatttca gatacaacaa tactttcttc
     7081 aatgggagct ggctgtaatc atatagacca tgttaacact caaatgccat atgctatatt
     7141 tacagcagta atcactattg tatttgggta tataccagct ggacttggat tgccaatata
     7201 tatagtatta ccagtggcaa tagctgctat atttgtaggt attcaaataa ttggtaagaa
     7261 agttgatgaa gcggaaatag aacttgtaga ataaagatat aaataagttt caccttttat
     7321 tatcactatt ttgttagttt ttagataatt ttaaaaagtt attgaaaata atggcgaaat
     7381 atagtaaaat aaaagagtga taagcggatg tggcggaatt ggcagacgca ctagacttag
     7441 gatctagcgc cattggcgtg ggggttcgac tcccttcatc cgcactttac agtacttaat
     7501 tgtactgaaa aaaactgggg ttgctggctt tcggagtggg ctatcccgtt tttatttttt
     7561 tgtatatata atagattggc actttagtat aaggtgcttt ttttataggc aaaaacagta
     7621 catttatttc tgttttatta tcattcatct attagaatat actttattta agaatgtgtt
     7681 aataagcagt gttcacattc ttgataaaat gtggaataat atgcctacca ataagcattt
     7741 ggtgtataag taagagtatt tatatagttg attgtgaaat gagtacaaca attctgtaca
     7801 gtataattgg cattataatt gtagcattga atttaaagtt tgcacagtcg ctgattggaa
     7861 aatttacaaa gtggatgtca aacatataaa ttttagttta ttggacagtt caaggaatca
     7921 gaaattggaa ttttatagta ttaatatttt atagtattaa tataatgtat aaaattttat
     7981 agcaatgtat ttcataagta taaagtgact agaattttaa gaaatataca aattctataa
     8041 aaactaaata atttcaagta tattttaaaa atcttatttt tcacaagttt aaaattgtaa
     8101 acgatttcat caagtctatg aatatacttt taacatgtta tagtaaatct caatataaat
     8161 ttacatttgg ttctttgtgt gttattttaa taataaatta agtgaaaatg ttattttaca
     8221 ttaatatata atatctttgc ataagagtga ataatataaa agagaaagaa ttgataataa
     8281 aaacttaaca aaaaaattaa tacataaaaa aaaataaata taatgtgttg acatttaatt
     8341 gaaatattca tacaataaaa ttgaaaataa atatttccaa atgagattag cgagagagtc
     8401 ctttatacga aaaggcgccg aagaagaaat ttaagaaatg ccaattttta gagaagcttt
     8461 caggcaaaaa tatcgctaat tgatgggact ctggagagac ttaagttaag atactataag
     8521 tcaccgaaga agcaaaataa tattaaatta ttaaatcttt caggtataca aacagggata
     8581 tataggctag gattattttc catgctgata tatctttttt ttatgaaaaa gtacaggaaa
     8641 taatatcaaa gttacttaaa tacagtaggt ctaattttct agggagggtt atatatgaat
     8701 gaattgaaaa gagtatcttt gtacaatata cacaaagagc ttggagcaaa acttgttgaa
     8761 tttgctggct gggaaatgcc tttagaatat gagggtataa ataaagagca tgagaaggtt
     8821 agaaaaagtg caggtatatt tgatgtatct catatgggag aggtacagat aaagggagca
     8881 gagtcagaaa aatttataca aaacctagtt acaaatgata taagcacatt aaagataaat
     8941 gacattatat atacacctat gtgttatgaa aatggtggag tagttgatga tttattaata
     9001 tataaatttg gtgaagaaga ttatttatta gtaataaatg caggtaatat agataaagat
     9061 gtagcatgga taataaaaca aagtgaagga tacaatgtag atataaaaaa tatatctagt
     9121 gaagtgagtc aattagcaat tcaaggacct aaagcagaag aaattcttca aaaaatcaca
     9181 gatatagact taaactcaat aaaattttat aagtcaattc catcaacaaa agtatgtggt
     9241 tgtccttgtt tagtttcaag aacaggatat actggggaag atgggtttga gatatactgt
     9301 aaaaataaat atgtagaaat aatttggaat gaagttctaa aggttggagg agaagatata
     9361 tgtccagctg gtctaggttg tagggatact ttgagatttg aagcagctct acctctatat
     9421 ggtcatgaaa taaatgaaca catatctcca attgaaggtg gtttatctat atttgtgaaa
     9481 acaaataaag aatcatttat tggtaaatca attttatcaa aagaaaaaga aagtggagct
     9541 aaaagaaagt tagtaggatt tgaaatgcaa ggtaaaggaa tgcctagaaa tggatatgat
     9601 ataaggattg gtgataaaac agttggattt gtaacaacag gatgtgcatc tccaacgaca
     9661 ggaaaaattt taggtatggg aattatagat tctgaatatg caaaagtagg aaatgaaata
     9721 ggtatagcaa tcagaaaaaa agttgttcct gctgtgatag ttaagaaacc tttttataaa
     9781 aaacaatata aaaaagataa tattatatta aacaaagaaa ataaattttc atatatacct
     9841 gctacaagtg aagataaatc aaaaatgtta aaagtagttg gattaaattc ggttgatgaa
     9901 ttattttcag atataccaga agaagtaaaa ttaaaaagag atttaaattt ggaaattgga
     9961 aaatctgaat tagaggtaag caaaatagta aaaagattgt cagaagaaaa cttaagttta
    10021 gaagacttga cttgttttct tggtgctggt gcatatgacc attatatacc atcaattata
    10081 aaacatataa cttcaagatc tgaattttat acagcataca ctccatatca agctgaaata
    10141 agtcagggaa ctttacaagt agtttttgaa tttcaatcta tgattgcaga aattacaggt
    10201 atggaaattg caaatgcatc aatgtatgat ggagcaactg ctgcaataga agcatgtata
    10261 atggcaatga atcagactag aaaaagtaaa atagtagtgt caaaaactat tcatcatgaa
    10321 actttgtcag tattaagaac ttacttacaa tataaagatt gtgagatagt agaaattgac
    10381 ttttgtaatg aatatgggac tacagatata gaaaaattaa aagcttctgt agacaaggat
    10441 acagcatgtg ttcttataca aactcctaat ttctttggaa ttattgaaga aatggaagaa
    10501 atagaaaaga taactcatga aaataaggct atgttaatta tgagtgtaga cccaatatca
    10561 ttaggagttt taaaaacacc aggtgagata ggtgcagata ttgttgttgg agaagctcaa
    10621 tcacttggaa accctcttaa ttttggaggt ccttatgtag gatttttagc tagcaaatct
    10681 aaatatacta gaaaaatgcc aggaagaata gttgggcaaa gtttagatgt agaaggtaaa
    10741 attgcatatg tattaacttt acaaactaga gaacagcatg taagaagaga aaaagcaact
    10801 tctaatatat gttcaaatca agctttaaat gctcttgtag cttcaatata tatggctact
    10861 atgggtaaag aaggatttaa agaggttggt atgcagtcta tgaaaaaagc acattatact
    10921 tacaataagc ttgtacaaac tggaaaatat aagccaatat ttaaaggtaa gtttttcaaa
    10981 gaatttgctg tacaaggaaa tcttaatata gaaactataa atgataagct cttagaagaa
    11041 aatatattag gtggttataa tttagaatac aattatccag aattaaaaaa ttcaactttg
    11101 ctttgtgtga ctgagaaaag aagcaaagag gaaatagata agttagttgg aataatggag
    11161 ggattataat gaaagaatac aatagtttgt tgatagatat atctaaaaaa ggtaggaaag
    11221 catactcact tccaaagtta gatatagatg atataaaaat agaagatatg attgaccaag
    11281 atatggcaag acaaagtgag ttgaatttgc cagaagttgg tgaattagaa ttagttagac
    11341 attatacatt attatcaaat aaaaactttg gagttgatac aggtttttat cctttgggtt
    11401 cttgtactat gaaatataat cctaaaataa atgaagatat ggcagcactt ccaaacttta
    11461 ctggaatgca tccataccaa tcttcggata cagctcaagg ttctctttct ttgatgtatg
    11521 atttatcaag aagacttgca gaaattacag gtatggatga agttacacta cagccttcag
    11581 caggttctca tggtgagttt acagggctta tgattataaa agcatatcat gaaaatagag
    11641 gtgaccataa gagaactaaa gtgataattc cagattctgc acatggaaca aatccagcaa
    11701 gtgcagctat ggcaaacttt gatgtgattc aaatagcatc tgataaaaat ggagctgtag
    11761 acataaatgc attaaaagag gttttgaatg atgaagtagc agctcttatg cttactaatc
    11821 caagtactct tggtttattt gaaaaaaata taaaagaaat agctactctt gtacatgaag
    11881 caggaggact tttgtactat gatggtgcaa acatgaatgc tattatgggg ataacaagac
    11941 ctggagatat ggggtttgat gtggttcatc ttaatcttca taagactttt tcaactcctc
    12001 atggaggtgg aggtccagga gctggtcctg ttggagttaa aaaagaatta gtaccttttt
    12061 taccaatacc attaattgaa agaaaagaag ataagtatgt actaaattat gatagagaaa
    12121 aatctattgg gaaaattaaa aatttctatg gtaattttgg agttcttgta agagcttaca
    12181 catatatact aactatggga agggatggac ttaaagaggc tagtgaaatg gcagttttaa
    12241 atgctaatta tataaaggaa agtataaaag atgattatat tttgccaata gataccttgt
    12301 gtaagcatga gtttgtatta ggtggattgc caagaggaga ggccaatata aaaactatcg
    12361 atattgcaaa aagactacta gattatggat accatcctcc aacaatgtac tttcctctta
    12421 ttataaatga ggcacttatg atagaaccta cagaaagtga gagtatagag acattagata
    12481 gttttataga agctatgaaa agtgttgcta aagaggcaaa ggaagaacca gaactattga
    12541 aaactgctcc acataatact ttggtcaaaa gagtagatga tgcaagggca gtaaagaaac
    12601 ctatactaac atggtcacaa agataaataa ttaaaaagta gagatttaaa gtataaagag
    12661 aattaaattt gtaagaaaaa gctatctaac ttaatccagt ttagatagct tttttgtata
    12721 ttagtatgta tggtattttc tatataaagt tttaaattat attagtgctt atatagaata
    12781 ttgatatgat gattatgtat agatattaat aagaattgtt gaatttatct tgccttaact
    12841 ttatttactc ttttatctta agaaaaactt aactaaaata tagcgaaatt cataa
//
LOCUS       DAMJVX010000102        12842 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMJVX010000102 DAMJVX010000000
VERSION     DAMJVX010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12842
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.106"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            123..311
                     /locus_tag="PC407_002477"
     CDS             123..311
                     /locus_tag="PC407_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDF2497915.1"
                     /translation="MAKVCSVCDKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(471..623)
                     /locus_tag="PC407_002478"
     CDS             complement(471..623)
                     /locus_tag="PC407_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HDF2497916.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(721..1197)
                     /locus_tag="PC407_002479"
     CDS             complement(721..1197)
                     /locus_tag="PC407_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2497917.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1416..2177
                     /locus_tag="PC407_002480"
     CDS             1416..2177
                     /locus_tag="PC407_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2497918.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            complement(2437..4365)
                     /locus_tag="PC407_002481"
     CDS             complement(2437..4365)
                     /locus_tag="PC407_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2497919.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITISRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQNNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            4599..5051
                     /locus_tag="PC407_002482"
     CDS             4599..5051
                     /locus_tag="PC407_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2497920.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGIATNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            5054..5362
                     /locus_tag="PC407_002483"
     CDS             5054..5362
                     /locus_tag="PC407_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2497921.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            5476..6582
                     /locus_tag="PC407_002484"
     CDS             5476..6582
                     /locus_tag="PC407_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2497922.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            6657..9335
                     /gene="mngB"
                     /locus_tag="PC407_002485"
     CDS             6657..9335
                     /gene="mngB"
                     /locus_tag="PC407_002485"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF2497923.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISNIENYIDSVKNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTANEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESSKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERSIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLLFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNNSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(9652..11985)
                     /locus_tag="PC407_002486"
     CDS             complement(9652..11985)
                     /locus_tag="PC407_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2497924.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGGA
                     KNPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKVINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLL
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(11960..12676)
                     /locus_tag="PC407_002487"
     CDS             complement(11960..12676)
                     /locus_tag="PC407_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2497925.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMRLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVNIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAINTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         4794 a         1789 c         1661 g         4598 t
ORIGIN      
        1 taagaaattc ttcaatatgt gtaaggcaaa tatatatttc tattgcaaat ttttgttatt
       61 tatgataata taaatatgtt tttagtcttt aaatagttat cattttaagg aggtgtgcgt
      121 agatggcaaa agtatgtagc gtatgtgata aaggtaaggt ttctggaaac caagtatcac
      181 actcaaataa acataataaa agaacatggt cagctaactt aagaagcgta agagcgatta
      241 tagatggagc tcctaagaga gtaaaagttt gtactagatg tttacgttct ggtaaaattg
      301 aaagagctta gtaaccacgt ttaaaagcct acttccgtaa gtaggctttt taatttaatc
      361 atttttactc tttctcataa attttaggat tatacttgaa atccattttg gtaattttat
      421 tgttactact ttcatcaata ctcaccccac actttatagt taaaactatt ttaacaaaaa
      481 aataataatg ctccacatcc aataagtatt aatgcggtaa gtgctgacca aatccagcca
      541 ggaagcaaca ttgacagaat tacagccatt cctgttgcta gacaaagtat tcctagcaat
      601 accttagact tgttatttct catggaacac ctccacatag aacctgtatc tataaatatt
      661 ttcatattca agtttatgcg acagttttcc attttagacc aataaacaca ttatgtaatt
      721 ttaatttttc atactagaat tattactgaa ctccgatata gattctacaa tatccaaaat
      781 tccttttttt ctacttaacc tttcaggtcc actatataaa accactggta taactttagg
      841 aagtttaaaa tttttatctt ctatttcttc aaggcttttt ttcataataa aacttttcca
      901 aatttgttct ctatacgatt tcaatcttgg gacaatttca gtatccattt ctgtttgaaa
      961 ttctaacact atataatgtg atactttgcc acttttagaa aataccttat aaattatatc
     1021 aacttctttt cctttatatt cacatataaa agatgtttct aactctatat ttttaagagt
     1081 taattctttt gctattgaga cacttgtaaa attttgaatc aaacttaaaa aactttcttt
     1141 atctgaatac attcttctgt actcttctcg tttttcattg cttcttttta aattcataca
     1201 gttcctccta ggctatagac cttctatatt ttagtaaaga tagtctataa attttatttt
     1261 ttaattttca ttagtgagta ttgacagttg tgtgtaaaat atccagtaaa gatatttttt
     1321 tatattttta ttaaaagata taaattttta tattttaata tattgacatt tacgttgcgt
     1381 aaaagtgtat atttaattta taggaggtat tacttatgga atatacagtt caaaaattaa
     1441 gtaaactagc tggtataagt actagaacac ttagatatta tgatgaaata ggtcttctta
     1501 agccacttaa aataaattct tcaggttatc gtatatatgg tcaaaatgaa gttaataaac
     1561 ttcaacaaat acttttttat agagaattag gaattaactt agaaaatata aaaaacatta
     1621 taaattcacc aaacttcgat agtttaagtg cgttaaaaga acaccataat aaattactag
     1681 ccaaaagaaa gcaaattgat ttactaatag aaaatgtaac taaaactata gctctgaagg
     1741 agggaaaata cactatgaca gatatagaaa aatttgaagg ttttaaagaa aagatgattg
     1801 atgaaaatga aaaaaattat ggaactgaaa ttagagagaa atatggtaaa gatattatag
     1861 atacatcaaa taaaaagctt aaaaatatgt ctaagcaaga ttatgaaaat tggcaaaacc
     1921 taaatatcga aataattagt aaacttaaaa aagcttttaa aactggtgat gcctctagtg
     1981 aactggctca ggaagttgca aaattacatc ataaatggtt gagttacact tggaacacat
     2041 actctaaaga agctcattcc tctctagcac agatgtatgt atatgatgaa cgttttacat
     2101 catattatga tagagaacaa cctggattag ctaagttttt aagggatgct atagtaatat
     2161 atacaagaga taaataatat aaaataataa aggtggtgtc tcaaaatgaa ctttttagtt
     2221 catgagacac tcttttttgt atgaaatcaa atatattttt atcattttat caatgaaaaa
     2281 caagtttctc tctctatttt gagacagaat ctttattgta taataattat aatcaataca
     2341 aaataataac aaattataca aaacttactt atactattta tgctaaaaac aaaaaatatt
     2401 ttattaaagt ttttaaaact tatgtaatat aaaaatctat actatagact tgattatgga
     2461 aagaaaatta tcaaaatcat tacaattaga tatactatct ctaatatcat tgtcatgaat
     2521 tatctttaaa aggatatcat aaatggagtt tatttttata taatcatctt tatttatagc
     2581 taacatcatg attaatttaa cagtagaatc ttcactccaa tatattggtt cattcaatat
     2641 ggaaatacaa atcttagttc ttatagagct taattccata ggatgaggta tagctaaaaa
     2701 atcatctata ttggtagaag ataaactttc tctgtaaaat acagattcag caaatgatgg
     2761 aaatacaatt tcactttgag ttaatttgtt gcacatcaac tttataagct catctttatc
     2821 tttgatttta ggattaacaa taaataaatt tttatcaaag aaattgtcca aaagattact
     2881 ataaatatgg gaattattag tgattgactt agatatattc tctatatctt tttttaatag
     2941 ttttaaatcc actaatacaa taggaatact atcatgattt aaaggaattg tcgatattat
     3001 tatatctaca tctgacaatt cactagctaa aaattgatta aatgatagac attgaagtat
     3061 atttatttta tcatgaaaaa ctttgtttaa ttgaacctct aataacctac ttgaaccata
     3121 ccctgaacca catactaata ctacgttttt acacttaata ttattttgaa aaaatctctc
     3181 tattccagca cctatatgca gggatacata tccaatttca tcttctgtta aagagtaaat
     3241 actattttta aaaacttttt ctatagcatt taaagttatt tcaaatgcta aaggataatt
     3301 actctttatt gtatttataa ggggattcac cttatttaaa ttataagatt tagaattgag
     3361 aattgattta aagtgcagta ctaaatcatc aaataataca ctatcatttc taaggtcaaa
     3421 agtatattga ttgttgatta cttctaaaat ttcttcaaca tactctataa ttttagtatc
     3481 caccctattg gaaacgttat ccaaaagaga acttttagtt ataaaatgat tatatagata
     3541 aactctgtct gcctttgaaa taattatagt atatttactc tcaatataat taaatatatt
     3601 ttcaacagtt gtattcgatt gaaaatctgt taaaacaaca tctaaaacat tatctaagca
     3661 gtaaccatca agtattctac ttatagtaat agctaaatgg ataataaagt tttttagatt
     3721 aaaatctaga aaatttatat taaatttatt aatttcactt aacactaccc tttgaagctc
     3781 tattaaatct acattattaa ataattctat ttctctatct gtaattccaa ttatatagtt
     3841 ttcaaaattc ctatttatca tattattaga aatacatctt cgaatatcta tttccttccc
     3901 ttcaacttta actccataat atggcttaga aactaatatt aaattatact ttgacaaaat
     3961 aggctttatt ctttttatgt catttagtat tgtaaattta ctcacataca agctattagc
     4021 taaatcttct agttttatat atgtattgtt atagagaata tattttaaaa tatattgatt
     4081 tctttctata ggagaatctg gtatttcact tgaatccata atatcatcag atattaatgc
     4141 tagatactgt tggtataatg ctaaattaag aatatctata taatatcctt ctcctttttt
     4201 tattttaata atagcaccat ttttctctaa agttccattt atagaagtaa tatcatttct
     4261 tatagttctc tcactcactt ttaaatgatt acttaaagtt ttacaagtaa ttttatcatc
     4321 actttcattt aataatttaa acaacgtgag caatctcttg tgcataaata tcccccctta
     4381 tagaatttct tctaaattat actatttaca ctcatataat acaattactc taataaaatt
     4441 gtattataat aatttctttc cacttctata tggaaattag ttattatcat aatataaatt
     4501 tccatgtcga agtggaaatt agttatattt aatttaatta ggataatgat tacaatataa
     4561 atataagtta agacattacg aatggagtgg aaaaatatat ggtactacaa gaaaaatata
     4621 tatttataga taaagactta aaaagcaaag aagaagtatt aaattttatt gcagatagtg
     4681 cttatatact aggtattgca actaataaag aggctctttt aaaagacctt ctaaaaagag
     4741 aaaatgaatt tccaacagga ataaaagatg gatttgcgat acctcataca aaaagtgtaa
     4801 atgtaaagga agcttccata ctctatatta aaacaactaa cgagttagag tggaaaactt
     4861 tagatgatag taaggttaga tatgtattta gtttgttagc cccacataaa aatgaaggaa
     4921 atatacattt aaaaactcta tctaaattag ccacatgttt aatggaggat gattttacaa
     4981 gtgaagttaa aaatgctagt gatgcaagtc atttagtaaa atatataact aataaaatga
     5041 aggaggaata attatgaaaa tagtagcaat aacatcatgt atagcaggat tagcacacac
     5101 acctatggca gcaaaagctt tagaacaagc tgcatcaaaa atgggtcatg aaatcaaagt
     5161 agaacaacaa ggtgctatgg gacgagtaaa tactataaca aaagaagaag cacaagatgc
     5221 ggactttata cttatagcat ctgaccaaac tatatctgga atggaaagat ttgatggtaa
     5281 aaaagtgata aaagtcaaaa taggtatagc gttaaaaaaa ccagaagctg tcttaacaaa
     5341 gtgtatagaa gctatttctt aataaaaaaa tctcactcta atatagaatg agaaaaataa
     5401 aaaattaaaa aattgtctaa tgtaattata acatattatc aaaaaatata acaaacttat
     5461 ataggaggat taaaaatgag taaaaaaaat aaatctcttt taagtgaact taagggacac
     5521 ttaatgacag gtatctcata tatgttgcca cttgtaatag gctcatcttt agttgtagct
     5581 atcccaaagc ttacagcttt agcaatgggt ataacaagtt tagatgctta taaaaatgta
     5641 tctggttttt atcatattct ttacttaatg gaacaagttg gttggactgg cataggtcta
     5701 ttaaatacgg tattagcagg atttatagca ttttcaatag gtgagaaacc agctatgggt
     5761 gcaggtttta ttggtgggct catagcaagt aatactaatg caggattctt aggagcagta
     5821 atagcaggtt tctttgctgg gtatgtttgt aagtttttaa agaaaaaaat taaaatatct
     5881 gggtcagcat caggaatgat gccacttata ataatgccac ttataacagt tggattaact
     5941 ggttttttaa tgagtgttgt attagcagga cctttaggaa acataaatac ttcattaaat
     6001 gcttgggtag ctgaaatgtg tcaaaatgga acaaattcgg tggtattggc tttaatttta
     6061 ggcgcaatga taggattcga tttaggtgga ccagtaaata aagcagcttg gatggcagga
     6121 aatgcattat tgttagaggg aatctatcta ccagcgatat tggtaaactg cgcaatagtt
     6181 atacctccac taggatatgg tttagctaca ttcataagaa aatcaaaatt ttccaatgca
     6241 ttagctgaaa caggaaaagg caatatagtt atgggtataa ttggaataac agaaggtgca
     6301 attcctttca ctttaacaaa tccattaaaa ttagttcctg ttaatatgat aggatgcgct
     6361 ttgggtgtag gattatcagc tttgttaggt gtacatgcta taatgcctcc agttggtgga
     6421 ttatatggat ttatatcaat aggttctgga tgggcttact tagtaggtgc aatatttgga
     6481 gctttagtaa tagcaatatt atcaacaact ttagtagact tcaatgaaga tgaaactacg
     6541 gaaaatacag aatttgatga aatagagtta gatataatgt aaattaaaaa cttaatttta
     6601 gataattgag aattgagtgt actctcaatt ctcaattaaa ttttgggagg atttttatga
     6661 aaaagacaaa agtacatgta gttccacaca gtcattggga tagagaatgg tattttacaa
     6721 catcaaggtc aaaaatatat ttaatgaaag attttcaaga tatattagac atattggaag
     6781 aaaaagagga ctttaaatac tttactatgg atgcacaagc ttctctctta gatgattatt
     6841 taaaatggag acctcaggat gaatatagaa taagagattt agttcaaaga agaagattag
     6901 ttattggacc ttggtatact caaacagacc aattggttat atcaggagaa tctataatta
     6961 gaaaccttta ctatggaata gatagatgca atgaatttgg tgaacctatg agggttggat
     7021 atgtaccaga ttcttttggg caatcagctc aaatgccaca aatatacaga ggttttggaa
     7081 tagatagttc attattttgg cgtggagttt ctgatgatat ggtagataca acagagttta
     7141 tatggaaagg ttctgatggt tcaaaagtat tagcagttca aataccattt ggttattact
     7201 atggaggtaa tataccagaa gatgattcag atttaaaaga atatttaaaa gatataattg
     7261 gaaaacttaa ggttaaagct tctactaata acgtatattt tccaaatgga tttgaccaag
     7321 caccaataag aaaaaacctt cctgatatat taaaaaaagc taatgaaata gataatgaaa
     7381 atgaatacga aataagtaat atagaaaact atatagattc agtaaaaaat gaaagaaatg
     7441 aatttacaga gcttaaaggt gaatttttaa atgctaaaca tatgagaata cacaaatcca
     7501 tattctcctc aaggtcagac cttaaaataa tgaataacaa attagaaaat tatatcacaa
     7561 atgtacttga accattatta acattaagtt attcacttgg aaatgaatac cctcatctag
     7621 ttataaaaga tatatggaaa cttatgtttg aaaatgcagc tcatgacagc ataggttctt
     7681 gtaatagtga cacagcaaat gaggatgtat acacaagata caaacaagcc agagatttga
     7741 gtataaattt attagatttg catatgagac ttataagtac tcaaatcaaa aataataatg
     7801 aagaaataac attaacagta ttcaatggat tcccagaagt cagaaatgaa gttgtagaat
     7861 ttgacactta tattccaggt caagattttg tgataaagaa taaaaatgga gaaattctta
     7921 attatataat aaaagaaaaa gaagatatta caaattatat attaactcaa acaataagat
     7981 taaacccaag taaaaaaata tatataccca aaactgtata tagagcaaaa attgcacttt
     8041 cagtaaaaga tataccacca atgggatata ctcaactagt ttttgaattg gatactaaaa
     8101 ataaatgtga aatagaaaca agtaattgca aagaaataga aaacaaatat tacaaaataa
     8161 cagccaataa aaatggttca ttagaaatat tagataaaga atctagtaag acttataaaa
     8221 accaagctgt aattgaagac aatggagatg atggagattc atacaactat tctcctccaa
     8281 gaaaagatat ttatgtttca tctttagatt ctgtatcttc agtagaagtg ctaaaatcaa
     8341 atattcaaga agaaatgata cttaaatatt cattgaccat accagcttca ctggaagaaa
     8401 ggtctatagg taaagtttct gtgaaaatgc cagtaacaat gaaaattacc ttagaaccta
     8461 aagaacaaat aattaagttt aatgttaata taaaaaataa ccaagcacta tctcatagag
     8521 taagagtttt atttaataca gaaatagcat ctaagttttc tatagcagat caacaatttg
     8581 gaataataca aagaccaaca gtattagaaa aagaccttgc attatggaaa agagacaatt
     8641 actcatggca agaaaaacct ataactatcg aaccaatgca aagttttgtt actttagagg
     8701 atggacaaag aggaatagca ttaataactg gttgtgtaag agaatatcaa atagttggtg
     8761 ataatttaga tacaatagca ttaacactat tcaggtcatt tggatatatg ggaagagaaa
     8821 atttacttta tagaccagga agagcttctg gagaaaaaat aatagcaaca ccagatgcac
     8881 aattacttaa agagttaaac tttgactttg gtctttacat atataacaat aaatttgatg
     8941 aagcaaatgt agctaataca gctaagagat tcttaacacc tatacaaata tatgaatatg
     9001 ctgatttttt aaatggtaga cttctatttg cattcaatga cgaaaaacaa atatatgaaa
     9061 atgaatttag tttgatgaat gtaaataatt caaatttcac agtaagtgca ataaagaaag
     9121 aagaaaaagg agatggaatt gtagttagaa tcttcaatgg tatgaaaaat gaggatgcta
     9181 agggaagtct taacatcaat aaaaatgtca atgatgctta tagtgcaatg ctcgacgaaa
     9241 tctatgataa taatagcaag cttaaagtaa atactaaaac tgtagaggta aattctttat
     9301 cacattgtaa agtacaaaca atagtaatta aataatataa aatagcttat acaagatttc
     9361 aaaataaatt gctagtacta tgttaatagt aaacccagat attatgctat ttataagctc
     9421 tgtaacacta aataacattt tgtaaaataa aaaggtgaac cttgtatatt aggattcacc
     9481 ttttttaaat aaatcaacat tctgttttaa aataacattt ataagctgtt ttgaaataat
     9541 acttacaaat tattttaaaa caatacctat aaatttttat ttaaagaata cacataaata
     9601 tttccagact cttttattct atagacttta taattattaa agaagtctaa tctaattttt
     9661 gttaaaagta attgtaactt taaataaatc accatccaca cttatattca tcttaccacc
     9721 ctgtaattct ataaaacttt ttgctattgc taaaccaaga ccagaacctt ctgtgttacg
     9781 tgatttatct cctcttacaa atctctccat tatatcatct acactaaaat caatctcaga
     9841 tgcagacata tttttaagca taatatttac tttattatct gtttctaaaa tatcaatata
     9901 cactcttgaa tttggcattg catatttgct tatatttatt ataagatttt caaatactct
     9961 aaatgtacgc tgactatcta atggtaatat tattttttca ttagaaaagt tattctttac
    10021 tattagagat gaatctgcaa gtttgtcatc taattctaaa agtgtctgct tcattagaga
    10081 tacaatatcc acatttacta tattcaaata aacatctcca ctatttgcct tgcttacttc
    10141 aaacaaatct tctattagat gttgcaatct ttgtgacttt ctgtctaatg tatcaatata
    10201 caacttacga ttttcatctg ttatactttc atcttttaat aaatctatat aagtgattat
    10261 agatgttaga ggtgttttta agtcatgtga tacatttgat ataagctctg ttttcatctt
    10321 ttgactctta acctcttcgt caacagcttt tttaaatcca gtttgaatat ttcctaattc
    10381 gtcttttata ggattaaaaa atcctaaatc tttttccata tttacatcta agtttccatc
    10441 tgaaattttc tttgtaacat ctaaaagttg attatattca tatattgtct ttttagagta
    10501 atctgacagt atcttaaata atactactga atatattaat gcgagtatta ttccaaagaa
    10561 ccacatacta cacattataa ctataatcaa taaatttatt cccaaaattg taactatagt
    10621 tttatttggc ttttctttta gatttattgt tctaatttga ttaataactg tttttcctgc
    10681 tttttttaac catacaaaaa acattcctgt cacagtatgt tttgtaaaat actctcttat
    10741 tccaacattt aaaatatgtt ttaaaagtat tataaataca aatattattg agaatattac
    10801 aaaccaataa gttaaattca ttaaacttac caatatgcta gccattttag cacttatgtc
    10861 attgtttata aggtactcta cataattccc actcaaggta ttacttatta taacagtagg
    10921 atataaatct acaagcccct gtataatagc aagagcaata aagagtatct ctaacggtat
    10981 tcttaataat gtatcaaatc ctagcaactc tttagatttt ctatatggga atgctaaagc
    11041 tacaataaca actatagcta atataatttt taaaatcatt ccagaaatct tataataaga
    11101 attattttct acattgctta tatatgaaat tataccatca tcaaaataag tatttccata
    11161 accatactct acactatcac ctttacttat tactttaggt acagcatata ctaatgttat
    11221 attttttatt ggagataact tatatgaaat tgaatttcct cctaaacttg tatccattaa
    11281 atcttctctt gctttagata acaacatttg attaataact ttttgattta ctccataaga
    11341 atccacaata tttacttttc catctttatc aaaattcatt acaacgtaga atctatagtt
    11401 ttcctttata tctgtaatct gcttttgtct ttcctcatca ttcatttctg tattttttga
    11461 ctgattttca tcagaatttt cttttgaagc atcattcttt gttgtatcaa gtactcctaa
    11521 ttctttatta tcattaaaaa attcaaaatt gctttcttta tcaactaaat agtactttaa
    11581 attctttcct gacgctttaa tgaaatgctc ccaattttca aagttttcat taaaatctgc
    11641 attaatacta tcatctgtat aataagtttc tgaaccatca taagtatctt tttctttact
    11701 tacatttttt atatagttca tagtttcttc attgataaat agctttgatg gttgtatcat
    11761 cttaccttct tgttcttcct tcatattatg atataaaaca taacttcctt tatatatact
    11821 atctacaaag ctttcctgtt caaatggatt ctttgctcca cctttagcac ctgcttgtat
    11881 agtagaatat tgactaatca tacctaatga tgctgataac ataagtataa aaactataat
    11941 agtaactata tatttatctt taaatttttt caattttgta tccaacaccc cacaccacct
    12001 ttaaatattt aggatttttt gggtcaattt ctatcttttc ccttatattc ctcacatgta
    12061 ccataactgt atctgtattt atagcatttt cattccaaac tctttcatat atttcatcag
    12121 cagaaaaaac tcttcctgca tttctcataa gtaaacttag tattttaaat tctataggag
    12181 ttgtttttat atattttcca tcaatattaa cttcttttgt attttcattt aattctaatc
    12241 cgcctattgc aaacacacta tcatcacttt ctaccttttg ctctttattt ccaaccatgt
    12301 ttagatactt agtgtacctt ctaagttgtg aattaactct agccaatagt tctaatggct
    12361 taaaaggttt tgttacataa tcgtctgctc ctatattaag ccccattatt ttgtcaactt
    12421 cttctgattt tgcagatagc attattactg gaaaattata gttttctcta agcctcattg
    12481 taagtgttat accatccatt ttaggcatca ttatatcaac tatagctaaa tgtatttctt
    12541 ggttttctat aaccttaaga ccttctaatc cattgtatcc cttaaaaaca ttatatcctt
    12601 gatttaatag atatatctct atagcatctg cgatctcctt ttcatcttct accaccaaaa
    12661 tattatatga attcatttta atctccttta tattaattta taaattttct ttacatatat
    12721 gattatacaa tatgatagaa gtttttccat ttatatatat atttatatct tttgttctgg
    12781 ttatttgtat ttaatgtcca tcttagaaac ctccttctag tttaaactta atattttttc
    12841 at
//
LOCUS       DAMJVX010000103        12825 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.107, whole genome shotgun sequence.
ACCESSION   DAMJVX010000103 DAMJVX010000000
VERSION     DAMJVX010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12825
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.107"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     regulatory      48..150
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            244..1620
                     /locus_tag="PC407_002488"
     CDS             244..1620
                     /locus_tag="PC407_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2497926.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCLGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFM
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(1734..2879)
                     /locus_tag="PC407_002489"
     CDS             complement(1734..2879)
                     /locus_tag="PC407_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2497927.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     SRGNRSGNTISIITKKELDIIKDLQKEFSIVITPKTLQNGELIDIIK"
     gene            3131..3838
                     /locus_tag="PC407_002490"
     CDS             3131..3838
                     /locus_tag="PC407_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2497928.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            3885..5480
                     /locus_tag="PC407_002491"
     CDS             3885..5480
                     /locus_tag="PC407_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2497929.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADSREKQKIMGMILSVVIITG
                     LLIFYVTSISLSLVDILKQINQMEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFFNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFMFIGQISSRIKYKNLILIIGSIILMLLYTLLMTRLNSMSVEMLKN
                     SVSISETIRKIYPPIYYFVDALKTGSVVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKTYKFKSQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIIGTILILL
                     FGADKVGELLKLNIDFNFIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLGFEIQFIIVSICLIASFSMFVALMGLLS
                     NLLYPNLDWKNEMAVVKRSASMIITMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TFILDFMIWRIISTKGVRIFKNL"
     gene            5583..6665
                     /locus_tag="PC407_002492"
     CDS             5583..6665
                     /locus_tag="PC407_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2497930.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            6814..6975
                     /locus_tag="PC407_002493"
     CDS             6814..6975
                     /locus_tag="PC407_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HDF2497931.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            7316..8584
                     /locus_tag="PC407_002494"
     CDS             7316..8584
                     /locus_tag="PC407_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2497932.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            8779..9567
                     /locus_tag="PC407_002495"
     CDS             8779..9567
                     /locus_tag="PC407_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2497933.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            9567..10016
                     /locus_tag="PC407_002496"
     CDS             9567..10016
                     /locus_tag="PC407_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2497934.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            10020..12284
                     /locus_tag="PC407_002497"
     CDS             10020..12284
                     /locus_tag="PC407_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2497935.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKNALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            12424..12735
                     /locus_tag="PC407_002498"
     CDS             12424..12735
                     /locus_tag="PC407_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497936.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         4705 a         1391 c         2184 g         4545 t
ORIGIN      
        1 attgacaaaa ttaattatat aaattaaaat aatattcgta aagaaaaatt catgttttgt
       61 actgtataac atataatttt gacaatatgg gtcataagtt tctaccggaa taccgtaaat
      121 attctgacta tgtatactat tatttagtta attaaatatt taattaactg aattatgaag
      181 gatacggctc agcttatcaa ctactgtgtc gtttttttgt aaaatttaat aggaggtgtt
      241 gttatgcaaa caacaaacag tagtactatg cagaaacttt ttccaatttt gactaataaa
      301 aatgtcgaca tgaagaagga aataattgca ggagtaacta cttttttaac tatggcttat
      361 ataatagctg taaatccaaa tattttatct gagacaggca tgcctgcagg tgcacttgtt
      421 acaggtactt gtttagcagc agcatttgga tgtattttaa tgggtgtagt tgctaacctt
      481 ccttttgctt tagcatcagg tatgggtctt aatgcatttt ttgcatatac agtagtttta
      541 caaatgggtg ttccttggga agtagctctt acagcagtat ttgtagaagg tattattttt
      601 atagtattat cagtaagtgg agttcgtgag gctgttgtaa atgcgatacc aaaaaatatg
      661 aaattagctg ttacaggtgg tataggaata tttattgcac ttataggtct agttaacagt
      721 ggaattgtta ttggagacca agccactctt atcaaaatgg gtagatttac accagcagta
      781 ataataactt gtttaggatt aataattatt gctgtattag ataaaaaaag agttaaaggt
      841 tctatacttt ttggaatagt agtgagttca ttacttgctt gggcatttgc ttttatgaat
      901 ccagagtatg cacaaaaatt aggtatatat ttacctggtg gattatttaa attcgaaagt
      961 ttagcaccaa tagcaggtaa aatagattta ggatatgtac tacatccaac aaatataggt
     1021 ggttttttag tagtagtttg tacattttta tttgttgatt tttttgatac agtaggtaca
     1081 ttagttggtg tttgttctaa agcaaacatg ttagatgaaa aagggaatgt tcctaatgta
     1141 ggtagagcct tacttacaga tgcggttgca actacagttg gtgcaggact tggagtatct
     1201 acagttacaa cttatgttga gagttcaaca ggagttatcg caggtggaag aactggatgg
     1261 acagcaataa cagttggaat tttattctta gctgcaatgt ttttctctcc tgtatttata
     1321 gcaattccat catgtgctac agcaccagct ttaatatatg ttggatattt aatgcttggt
     1381 acagttaagg atatagagtt tgataatata actgaaggtg ttccagcttt tgttactatt
     1441 gcttgtatgg cattaacata tagtataggt gatggtttaa ctttaggtat attaacttat
     1501 gttttcgtaa atatatttta taatatattt ggagctaaaa aggcagaaga caaaaaacat
     1561 gtttctgtag taatgattgt tctggcaata ttatttataa taaaactttg gctattataa
     1621 taggtattat ttaaaatttt aatttaatat aaaataaagg gctataattt attatcaatt
     1681 gtgatgataa actacagctc ttttgtattt gttgtatttt cgtatttatt gtattattta
     1741 ataatgtcta ttagttctcc attttgtaaa gttttaggtg taattactat actgaactct
     1801 ttttgcaaat cttttataat atctaattct ttttttgtta ttattgagat tgtgttacca
     1861 cttcgatttc ctctagaagt tcgaccacat ctatgcaggt attcattttt actttttgga
     1921 aaatccaagt taaaaacatg acttacatcc acaatatcta gtccacgagc agataaatca
     1981 gttgtaatta aaatctttgc tttaccaagt ttaaatttat ttattgcatt ctttctatct
     2041 tctttttcca tatttccaaa aattcctatt gctttgtaat tatgataatt tagttttgat
     2101 actaggactt ctatattttt ttcattattt acaaatacaa tagctttttt tggatttgta
     2161 gctgctaatg cctttctaag aaaagtaaat ttatccctta tctcacctaa taaatatgag
     2221 tgattgatgt taggatttat ttgtgatttt tcctttgttt ttattatttc aaactccttc
     2281 ataagtttat cacaaatttt tatagtgcta tcagtaagac ttgcagagca tcctataatt
     2341 tgtctatctc ttaacgttgt tcttattatg tcttctatac aagttatatt ttttccattc
     2401 aatagattgt ccacttcatc taagactata gtttttatat tatgtgattt tagcttcttt
     2461 tgttttatca agtctaaaac tcttccacaa gagccaatta ctatgtgtgg ttttactgct
     2521 tttatatttt ttatttgttt ttgtatattg acctccccaa ttaaagctaa agaagtaacg
     2581 gagagttctg catttttagc caataattca acctggtttg taatctgcat tactaattca
     2641 tgcgtaggtg caagtataag agcttgtgtt tctctttttg atgtatcaat tttttcaaat
     2701 ataggtaaaa ggtacgctag tgtttttcct gttccagtct gtgaattaat caataaatcc
     2761 ttattttgaa ttatattccc tataacaagt gactgaactt ctgttggaga agttatgtct
     2821 tgttttttaa gtccatctat tagagtggaa cttattttta attgttcaaa tgtattcata
     2881 ttttgctcct tatatttaat aattatagtc aactattttt tattatacat tgtttattca
     2941 aatcgaacta tatattcttt tataattatg tgtaatttga aagtaaatat caattatatt
     3001 tataccttta tatttctgat acctttgcat tttatagatt tctaaaataa acaaataaaa
     3061 gttgtaattg tttgatattt gttatatatt atatataaca aatatcaaaa ctataatatg
     3121 gagggaaatt atgttaaaag taatcaatgt atctaaatca tataaaaata aaaaagtagt
     3181 aagtagtgtc tcatttgatg taaatgatgg agagcttttt gggtttatag gacataatgg
     3241 agcaggtaaa actactacta ttaaagcaat tgttggaata cacgattttg aagagggaga
     3301 agtactaata aattctaaat ctataaaaaa acagcctgtt gaatgtaaaa aggaaatggc
     3361 atatatacct gataatccag atttatacga gtcattaacg ggtatacaat accttaattt
     3421 tatagctgac atatttgaag ttgccaaaga caaaagagaa gagttaataa aatattattc
     3481 aaataagttt gaactcaata aagcattagg agatttaata tcttcatatt cgcatggtat
     3541 gaaacaaaaa ttagctataa tttcggcttt gatacacagc ccaaaaatct taattcttga
     3601 tgaacctttt gtaggtcttg acccaaaagc ttcctttata ttgaaggaaa ttatgaggga
     3661 gttttgtgac aaaggtggtt gtatattttt ctcaacacac gttttagaag ttgctgaaaa
     3721 aatttgtgat aagattgcca taataaaaga tggaaatata attgcttatg gaactacaga
     3781 agaagttaag ggaagtaatt cacttgaaag tatctttatg gaattaatag agaaataaaa
     3841 tgttttttat acaatatagt gtctaaagtg aggaatgata aaatatgagc aatctaagta
     3901 tattaataaa aactaatata ataaatgaat ttaaattaaa tgcgttaaaa aaagctgata
     3961 gtagagaaaa acaaaaaata atggggatga tactttcagt tgttattata acagggttat
     4021 tgatttttta tgtaactagt ataagtcttt ctttagttga tattttaaag caaataaatc
     4081 agatggaaat gctacttata tttggatttg gattatcaac attaacaact attgttacat
     4141 cattatataa aacgtcatca tatctgtttc aagcaaaaga tttagacctt ttgactagtt
     4201 tacctataaa agaatctact gtactagcaa gtaagatatt aatgttggtt ggagcaaact
     4261 atttattttc ttcaatatgt atgattatac cagcagtggt ttattttttt aattctgatg
     4321 taagtataac gtatttccct tatttgatac tgctattttt agctttacca ttattaccaa
     4381 tagttgcttc atcaataatt tttatgttta taggacagat atcatcaaga ataaaataca
     4441 aaaatttaat attaattata ggtagtatta tattgatgtt gctttatact ctacttatga
     4501 caagacttaa ctctatgtcg gttgagatgt taaagaatag tgtatctatt tcagagacga
     4561 ttagaaaaat atatccacct atatattatt ttgttgacgc tcttaaaaca ggaagtgtag
     4621 tttccttttt agcatttata gcaatttcaa taataccatt ttttatgttt gtaacattat
     4681 ttgcaaaaaa atttaaagat ataaatgcta agatgagtga aggatataag tcaaaaactt
     4741 ataaatttaa gagccaaaaa acttcaagac cttcaaaagc tctattaaaa aaagaattta
     4801 agagatatac ttcttcattt atatactttt taaattcatc tgcaggtttg ttattcctaa
     4861 taataggtac aattttaata ttattatttg gagcagataa agtgggtgag ttactgaaat
     4921 tgaatataga ttttaatttt ataaaaatac aattattggg agtattcttc tttataatag
     4981 taatgaattg tacaacatac tgttcaattt ctcttgaagg aaaaaattta tggatactga
     5041 aatcttctcc aatagatgaa agagaaatat tctggtcaaa aatattgatg aattttttat
     5101 tgaatgcacc attatctatt ttatgtttaa tattagtatc tataagatta ggttttgaaa
     5161 ttcagtttat tattgtatca atctgtttga ttgcaagttt ttcaatgttt gtggcattga
     5221 tgggattatt atcaaattta ttatatccaa accttgattg gaaaaatgag atggctgtag
     5281 taaaaagaag tgcaagtatg ataattacaa tgttagtatc attagcttac gtaattatac
     5341 ttggaggtat atatgtatta cttggaattt cgtttttaaa tatatatatt attttggcaa
     5401 gtatacttac ttttatatta gattttatga tatggagaat tatttctact aaaggagtaa
     5461 gaatatttaa aaatttatag gtgtatttat gtcaaataaa gacttatctt ttttagtctt
     5521 gcaaataata ggttaagtag tatactatgt atagtgatat gaaaaaattt aggagaaaat
     5581 aaatgagaaa attatccaaa agagaaaaag attttattca atatggattt ttattatttt
     5641 tgatatgtat tactacttat ttagtattaa ctactttaga tataaaacta atacctaaaa
     5701 ttataaggct agtaaataag acttatatat ttcttggcat attgacaatt atagtatata
     5761 tgataattga atctattgta actcatttga taataaatgc agttcaaaaa acaaaagtaa
     5821 aatttattgg tattaaaatg gctacaatgg gattttacta taacttagtt actccatttg
     5881 catctgggag tcaaccaatg caaatatatg cacttacaaa atatgatgta agccttagta
     5941 aatcagtggc tatagttact aataaaactg tagtatttca aagtacagtt actgtatttt
     6001 gtggaatttt aattttgcta aataagtcat tactgatgga gcaagtacat tctgtgaaag
     6061 tcttgataac ttttggaatg attatgaatg taagtatgtt gttaggtgga ctattaatag
     6121 tatttagtcc aaaacaagta aaaatgatag cagaagtttt gattaatttt ttatctaaat
     6181 ttaaaacatt taaattttta gattcaaaac gtgatagaat aaatcattat attgatgatt
     6241 ataattattc tataaaaata tttattaaaa atgtaagaat cttagtttta agtatattac
     6301 ttactgtgtt acaattaatc gcatatttca gtatagttta ttgtgtgtat aaagcctcaa
     6361 atttaagagg agtttcatat gtacatataa tgacattaca ggtcttttta tatatggcta
     6421 tatcaccaat tccaacacca ggaaatgttg gagctaatga ggttacattt tttactatgt
     6481 tctccaatat aattccaaaa gaattattgg ggtactctgt actcttatat agtgggtttg
     6541 tatactattt tatattaata gtttcaggtt tatttacatt tggaacgcat tatacattaa
     6601 attcttggaa aaaggaagat aaagtaaatt ctaatttaga taaggttatt gaaaaattaa
     6661 attaataaaa taattaggga aattctatat ataaatatgg tgttgagtaa gctatattat
     6721 attgagatat ttcccttttt tgattaaaat tatagaaatt tgaaataata agtaataaac
     6781 agaaaaagtg atattgtttt gaaaggagaa actatgaaac aagataaaca aaatgaaaaa
     6841 caaagtgaga tttctaagat aaaacaaagc aatgataata aacttgaaat agctcaagaa
     6901 ttacatgtaa gtaaaaatat agggaatatg cttggatata gaatagataa gaaaaaaatg
     6961 aatagaccaa gttaatgaaa atgttgaaaa tggtacattt taaaaaatat atataatgaa
     7021 atgttttatt gttaaattta aacttatttt aatggaggaa aaagtcgaaa aatatctaaa
     7081 aagatatgga tataaaaaat attaatatat atatactata aacttgacaa aatcaacctt
     7141 ttaataacta gcaaaaaata gcaaaattat ataagcgtta tttaattatc aaaaatgaaa
     7201 gctaaattgc atgtcctaaa aaaaatgatt gataacattt atttgtgtgc tataattata
     7261 tagtaaaaca ataagtatat tgcataatgt atgcaaatcg ggaggagaga cggacatgga
     7321 agcatctata cacacaattg ccagcaataa tttattgata atttttgcta ttgtaagtat
     7381 aactggaata atttgtagta agcttagtga aataataaag gttccagatg tagtgttata
     7441 tttacttgta ggtattttaa taggaccatc atttttaaaa tttatagata ttcgtggatt
     7501 tcaaatagaa aatcaactaa tacttacatt tggttctgca tttattttat atttaggtgg
     7561 aaaagaaata agtttaaaag tattgagaaa tgtgaagata tctgtattct tattagctac
     7621 tttaggagtt gtgatatctg cttttataat gcaacaagta ataggattta cttttgggat
     7681 aagtgcaatg actgctttac tagcaggaac tataatagca tcaacagacc cagccactct
     7741 tgtacctata tttaatcagg taaagataaa ggatagggta aaacaaactg taataagtga
     7801 atcagctttt aatgatgcaa caggagctat actaacatca gcagtgttgg ctgtaatttt
     7861 gtcaaataaa ttttcattag gtgaaaatat ttatgaactg gggacaatgg ttattgtcgg
     7921 agttttagtt ggtttaatta caggaatact gcttttgaaa ttagttaatg ataaacctta
     7981 tggaatattt aaagattttg caccaataat atctgttatt tcagttgtag ttgcatatga
     8041 attatctaca aaattaggtg gaagtggata tatgtcttgc tttattgtag gtataataac
     8101 tggtaacaag aaaaacttta aaatttggtt aagtcaaaaa tcttatgatg cagattttta
     8161 tgttgctgaa actttaggta ctatttgtcg tatggcaata tttataatat tgggtagcca
     8221 agttaatttg gctcagttaa gtaaatattt tatgccatct gtaattacag tattagtatt
     8281 aatgtttata gcaagaccac tttgcgtttt agtttgtaca cttgtagaca gggaagcaaa
     8341 atggagtaaa agtgaaatat tatttatgat gtgggtaagg gaaacaggcg taataccagc
     8401 agcactttgt ggtattattt cagcaatgaa agttcctgga tacgaagtga tttcatctgt
     8461 agtatttatg actatattaa ttacattagt aatacaagga agtactacaa aattagtagc
     8521 taaaaaactt ggattattag aagaagaagt gataaatata agtgaaaaag tatcaacttt
     8581 ttaaagtttt gctttaaaat agtacctgtt aatggctatt ttaatattgt ctaatgatga
     8641 ttattgaaat agtcattttt ttatgtataa gtaatttaca gaatttacat attagacata
     8701 tttattatca aaatttgttg aagctatgat ataattagtt gtatttaaaa taagaatagc
     8761 aaataaggga ggaaatttat gtttacggtt atggatattg tacagccaga tacagtagaa
     8821 gaagcgtact caattttaaa taaaagaaaa actaaccaag taattggtgg aagtgctttt
     8881 ttaagaatgg ggaaaaaaag aataggaaca ggaatagaat tatctaaatt aaatttagat
     8941 tatataaaag agtatgaaga ttacgttgag attggttcta tgactacttt tagaacatta
     9001 gaaacaagtt ccataataaa aaataatttt ggaagaataa tagaagattc agttaaaaat
     9061 ataataggag ttcagtttag aaatgtagta acagttggtg caacagtatt ttctaaatat
     9121 ggtttttctg acttgatagt agcattactt tcattagata cggaagtaga attatataat
     9181 attgggagaa ttagtcttga agagtttcta aatagagatt atgagaaaga tttacttatt
     9241 aagatataca taaagaagac aaataagaat gcttcttata aatcacttag aaatgcaaag
     9301 agtgattatc caattctaaa tgtatctgta tcaaaacata tggaacaatt taagttatgt
     9361 gtaggagcaa gaccgcagaa agctacgatt gcaaaacaag ctagtgaatt tctatctaac
     9421 aatgaaatca atgaaattaa tatagataaa gcagtagaaa tagcatcaga agaactaact
     9481 tttggttcta acatgagagc atctagggag tatagaaaag ctatgagtaa agtactttta
     9541 aaaagagcta taatggaggt tatttaatgt tagtaaagat taatataaat gggaagaaaa
     9601 aaagtgttga tatagaacca gaagaatatc tggttgatac tttaagaaaa gttggtaatt
     9661 taagtgtgaa aagaggttgt gacacaggct gttgtggttt atgtacagtt ttaattgaca
     9721 agaaacctac attatcctgt gcaacactga cattgagagc tttagataaa gaaataacta
     9781 caattgaagg tttagaaaat gaagttaaag aatttagtga agttttggta aaagaaggag
     9841 ctgagcaatg tggtttctgt tctcctggct ttatacttac tgtacttgca atgaaggatg
     9901 agcttgaaaa tcctacagat gatgaaataa agcattatct tacaggaaat ctatgtagat
     9961 gtacagggta tatgggacag ttaagagcta tcaaaactta tctggaggta aaatagaaca
    10021 tgaatatagt tggaaaagga atcaagaaaa tagatggaat gagtatcaca acaggaaagc
    10081 ctgtatatac agatgattta tcagcaaaaa atgctttggt ggtaaagata cttagaagtc
    10141 ctcacgctta tgcaaaaata aaagatatag atacatcaaa ggcagaaatg gttgatggtg
    10201 ttgaatgtgt tttaacatat aaagatgtac caaaatctcg atttacactt gctggtcaaa
    10261 catatccaga gccatctcca tatgatagat taattcttga agatgttgta agatatgtag
    10321 gtgacgaagt agctatagtg gcagctatag atgaaaaaac tgcaattaaa gcaatgaatc
    10381 ttatcaaagt aaagtatgaa gtacttacac ctgttttgga ttttgaagag gcaataggaa
    10441 ataaaactat ggtacatgat gaagatagac attgtaactt tgatataggt atgcaaagag
    10501 aaaaaaatat agtatcccat catgattata caaaaggtga tgtagaaaaa gtattaagta
    10561 attgtgatgt agtgattgaa gaaacttatt atactcaagc tcaagcacat gcaatgatgg
    10621 agacgtatag ggcatacaat tatttagacc atatgggaag attggttgta gtaagttcaa
    10681 ctcaaatacc atttcatgta aaaagacatt tatctcgtgc actagaaata ccagctagca
    10741 aaattagggt tataaaacct agaattggtg gcggatttgg tggtaaacaa actgcgtgtg
    10801 ttgagatttt ttctgcaatt gttactttaa aaacagggaa accagctaaa ataatttatg
    10861 atagaaaaga aacttttaat tgttcaacaa gtagacatgc aatgagatta aaagttagac
    10921 taggtgctac aaatgatggc ataataaagg caatagatat aaaagcactt tctgatacag
    10981 gagcatatgg agagcatgct tctacaacta ttggtttggt tggagaaaaa actttaccaa
    11041 tgtataataa agttgaagca acaagattta ttgcagatgt ggtatatact aataagatgc
    11101 cagcaggagc acttagaggg tatggagcat cacaaggatg ttttgctgtt gaatcaacta
    11161 taaatatgct tgctaataaa ttaaaaatgg acccaacaga tttaaggtta aaaaatgtgg
    11221 taacagaagg tgaaactaca tttgcatatg gtaaaacttt aaatagtgtc gatttaataa
    11281 aatgtataaa taaaggcaaa gaacttattg gatggaatga aaaatatcct tgtaaacatc
    11341 ttgataatgg aaaaattaga agtgtgggta tggcaattac tatgcaaggt tctggtatac
    11401 aaggtcttga taagtcttca gctgaaataa aattgaatga ctgtggagat tatactctta
    11461 tgataggttc tactgatatg ggtacaggaa gtgatacgat tttagctcaa atggctgctg
    11521 aggttcttga tacaagcatt gaaaatataa cagttatatc agctgatact gacttagttc
    11581 catatgaccc aggttcatat gcttcaagta ctacttatgt aacaggtatg gcagtagtta
    11641 aggcagctca agacttaaga gaaaaaataa tttctgctgg agcaactaaa ctaggtgttg
    11701 agaaggatga agtagaattt gatggtataa aattaactag taaaaaaggt agcactttat
    11761 tattcgatat agcagttgat tctacaattg gtccagatac taatcaatta ataggtcttg
    11821 cttctcatgg tagcccagta tctcctccac cttttatagc aggttttgca gaaactgaaa
    11881 ttgacacaga aactggaaaa gttgatttag taaattatgt agctgtagta gattgtggaa
    11941 cggttataaa taaaaatcta gctaagatac aagttgaagg tggaattgtt caagggattg
    12001 gaatggcatt atttgaagaa gttagataca cagaaaaagg aagcatggac acaaatacat
    12061 ttatgcaata caagattcca gctagatgtg atattggaaa tgttaaagta gattttgttg
    12121 aaacttatga accaactgga ccatttggtg ctaaatctgt tggagaagtt gttataaata
    12181 cacctttacc agcaatacaa gaagctgtgt ttaatggaat tggtgtaaga ataaatacac
    12241 tccctattac acctgaaaaa gtttttatgg aaatgaataa ataattattt atgaaaattt
    12301 agactagttt tacatgttaa acaaccagtt atgtcatacc tgttgaaaga tatttaataa
    12361 gtagttatta taatattatg aattttactt ataaaataca aaattaaata aggggatgat
    12421 gttttggctg gtaatctaaa taatatgaga gcagtaaata attttagagg agataagaac
    12481 attttagaat gtttagtcag ctttgagggt cgttcaataa gtcagagaaa agtaagggta
    12541 ttttttaaag aaaaacaaaa tcaaatagaa attgattttg cagaagagga aatttctaaa
    12601 ttggttgaaa atgttgtttt aaatacatca tatcaagaaa tgttatatga tgaaatagag
    12661 aaacaactgg aaattgattg tataggtact tggatgatat tatctaaatt aaaagatggt
    12721 agtagagttc actgatttta aaataaatta ttagagccta attttaaggc tcttttttgc
    12781 tgtttttaat taacaataga aaatttatct ttagtttgta tataa
//
LOCUS       DAMJVX010000104        12637 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMJVX010000104 DAMJVX010000000
VERSION     DAMJVX010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.108"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..390
                     /locus_tag="PC407_002499"
     CDS             <1..390
                     /locus_tag="PC407_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDF2497937.1"
                     /translation="YSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIY
                     KQALIKKEIDLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPK
                     PQKKLDLNESLILNNLKNLEKQYNSSY"
     gene            403..915
                     /locus_tag="PC407_002500"
     CDS             403..915
                     /locus_tag="PC407_002500"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDF2497938.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            989..3196
                     /locus_tag="PC407_002501"
     CDS             989..3196
                     /locus_tag="PC407_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDF2497939.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            3221..3670
                     /locus_tag="PC407_002502"
     CDS             3221..3670
                     /locus_tag="PC407_002502"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDF2497940.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNASRPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            3685..4299
                     /locus_tag="PC407_002503"
     CDS             3685..4299
                     /locus_tag="PC407_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2497941.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVSDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            4390..5946
                     /locus_tag="PC407_002504"
     CDS             4390..5946
                     /locus_tag="PC407_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HDF2497942.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNRLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDKKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEELNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            5936..7198
                     /locus_tag="PC407_002505"
     CDS             5936..7198
                     /locus_tag="PC407_002505"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDF2497943.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            7272..9059
                     /gene="aspS"
                     /locus_tag="PC407_002506"
     CDS             7272..9059
                     /gene="aspS"
                     /locus_tag="PC407_002506"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDF2497944.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTKMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDIQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            9518..10750
                     /locus_tag="PC407_002507"
     CDS             9518..10750
                     /locus_tag="PC407_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2497945.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            10764..11570
                     /locus_tag="PC407_002508"
     CDS             10764..11570
                     /locus_tag="PC407_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HDF2497946.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKHGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(11682..12551)
                     /locus_tag="PC407_002509"
     CDS             complement(11682..12551)
                     /locus_tag="PC407_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2497947.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
BASE COUNT         4962 a         1378 c         2363 g         3934 t
ORIGIN      
        1 tattcgtata tatatgagaa taaaagagaa gaaagtgaaa ttataaaata ttataataaa
       61 acagacctat tatatttaaa gaatgtagtt agtaatatag ttccatcaag agatgaattt
      121 ataacaattt ataaacaagc acttataaaa aaagaaattg atttggatat ggtaaatata
      181 agggaaactt ttaatgtaat tccattaaag ttttttacga tattaaatgt atttagagaa
      241 ttaaatttac ttgactttaa tttaaattat gaaaaaaatt ctgtactcat aagaatattg
      301 ccaaaaccac agaaaaaatt agacttaaat gagagtttaa tactcaataa tctaaaaaat
      361 ttagaaaaac aatacaatag cagttattag gaggcgtttc ccatggattt aaaaaacttt
      421 ataaggaata ttgatgattt cccaaaacca ggcatagatt ttaaagatgt gacaactcta
      481 tttaaagatg gagatgcatt taaatatgca gtggattcta tagttgaaga attgaaagat
      541 aaagatgtag atttagtaat agggccagaa gcaagaggat ttttgatggg tacacctgta
      601 gcatatgctt taggagtagg atttgttcca attagaaaac ctggaaagtt accaggtgaa
      661 gttgagagtt atgagtatgg attagagtat ggtacagata ctttagagat acataaggat
      721 gcaattaaaa agggtcaaaa agtagcaata gtagatgatt tattggctac tggtgggact
      781 atggaagccg ctgccaagct tgtagaaaaa ttaggtggag aagtagtttc tatgcaattt
      841 ttgattgagc taaaattctt aaatgggaga gaaaagttgt ctaactatga tgttaattct
      901 cttataaaat actagtactt ttataatagt tggtataaca ccaactattt tcatatatat
      961 taaaatttta tacaaaatag gtggttatat gcatgataaa gaattacaag aaataattga
     1021 aaaaataaaa gtttatgcac ctaatgcaaa ttttgaaatg gttgaaaaag cctataatct
     1081 tgcaaaaaaa gcgcatgaag gacaatatag aaaatctggg gaaccatact ttattcatcc
     1141 attagcagtt gcaaatattt taatagagat ggaattagat attgaaacta taacagcagg
     1201 tttacttcat gatgttgtag aagatacaga atatacctat gaagacataa aaaaagaatt
     1261 tggcaaagaa gtagcggatt tggttgatgg agtaactaaa cttggtcaaa taaaatatcg
     1321 ttcgaaagaa gaaactcaat ctgaaaattt aagaaaaatg tttttggcaa tggcgaagga
     1381 tataagggtt atacttataa aacttgctga tagattgcac aacatgagaa cattaaaata
     1441 catgcctcca gaaaaagcaa agtataaagc aaaagagaca cttgaaattt atggtggtat
     1501 agctcataga ttaggaattt ccaagataaa gtgggaacta gaagacagag cattaagatt
     1561 tatggaccca gaagggtatt atgatttagt tagtagagta tcaatgaaga gaagtcaaag
     1621 agaggattat attcaaggaa ttgttgaact tcttaaagat aagtttaaag atttaagtat
     1681 accatgtgat gtatatggta gaccaaagca tttttacagt atatatagaa agatgcaaaa
     1741 aaaacataag acttttgaag agatttttga tttaacagca gtaaggatat tggttgataa
     1801 tgtaaaggat tgctatgcag tacttggtat agttcataca ttatggagac ctattccagg
     1861 aagatttaaa gattatatag caatgccaaa acccaatatg tatcaatcat tacacaccac
     1921 tgttgtaggt cctgatggag aacctttaga gatacaaata agaactcatg agatgcataa
     1981 tatagctgaa aatggtatag cagcccattg gaaatataaa gaaggtatct ctagcaaaga
     2041 gtcaaaggtt gaagaaaaac ttcaatggtt gagacagatg atggagtggg aaaaagattt
     2101 aaaagaccca caagagttta tggatgcact taaagaagat gtatttaata gtcaagttta
     2161 tgtatttacg ccaaaaggag acgtaattga attaccagca gaatcaacac ctatagattt
     2221 tgcctataga gttcatacaa atgtaggtaa taaatgtgta ggagctaaaa ttgatggaag
     2281 aatagttcct attgattata agcttcaaaa tggtaatatt gtcgaggtac ttacatcatc
     2341 taattctaat ggaccaagta gggattggct taatatagtg aagaccccaa atgcaaaaag
     2401 tagaattaga caatttttca agaaggaaag aagagaagaa aatgttgacc gtggaaatac
     2461 aatattagaa agagaattta agaagcatga tataccacaa aaagaccctc ttgttgaaaa
     2521 atatatggca ttagttgcta agaaatttaa tcagccaagt gttgaggact taattgccac
     2581 tgtaggttat ggcggtataa tgtcatctca agtggtatct aaggtaaaag acctatattt
     2641 aaaagaggtt aaaaaagcta aaaaggaaca acagatacaa gaagaagatt taaataaaca
     2701 caatataagt gaaacagaat acagtaaaaa gagaaagaaa aacgcatcac aaggaattat
     2761 agtaaaaggg cttgacaata tcttagtaag atttgctaag tgttgtaatc ctcttccagg
     2821 agatgaaatt gtaggatata ttactaaggg taggggagta gccatacaca gagctgattg
     2881 cccaaatgca aacaataatg gtgatttctt ccaaaataga ttggtggagg tttcatggag
     2941 ttcagcagga aaaggtaagt ttgaagcgga aattcaaata aaagctattg acagaagagg
     3001 aattgttaat gaaataactc atattgttgc aatggataaa cttagtttaa acggaataaa
     3061 tgcaagaaaa ggtaaggata gtattgtaag tataaactta cttgtagagg ttaatgacac
     3121 agaggaatta aaaattctaa tgaaaaaatt aaaagcaatt cctggtgtag aaagtattta
     3181 tagaatgaca aattagagtt aaaataatag ggaggaaact atgagagcag tagtgcaaag
     3241 agtatcttca tctaaagtta cagtagatga aaacactata ggtcaaataa ataaggggtt
     3301 actagtattg ttaggagtga ctcatgatga taaatcaagt gatgtagatt atatgattga
     3361 taaaatattg aatctgagaa tttttgaaga tgaaaatgac aagatgaatt tatctctaat
     3421 ggatataggt ggagagcttt tagtagtatc tcaatttact ttatatggag attgcagaaa
     3481 aggaagaaga cctggattta gtaatgcatc tagaccagaa cttgcaaata acctttatga
     3541 agaatttgta aaaaaagcaa aagataaagg tgtgacagtt ggtacaggtc aatttgcagc
     3601 tcatatgatg gtagagttaa ctaatgatgg tccagtaaca atactattag actcaagtaa
     3661 atctttttaa gggggaaaac tttaatgatt ataaaaaaat ttgtagatag ttattttgga
     3721 gtaaatactt atgtattagg tgatgaaaag actaaaaaat gtgcagttat agacccaggt
     3781 ggaagtttag tagagacatt aagttttgta aaagaaaatg aattaaatat tgaatatatt
     3841 atccttactc atgggcatgg agaccatata ggatatgtta aggatattaa agaaaaaact
     3901 ggtgctaaag tagttgcaca tgttgatgaa aaagaattat taaatgataa aaataaaaat
     3961 ctaagttata caatgagatg tggagcgcaa gagtttgatg cagatatcta tgtgagtgat
     4021 aaagacaagc ttgaacttgg tgaattaaaa atgactttct tgcatactcc aggacatact
     4081 caaggatgta tgtgtataag agttgaagat gaaatgttca caggagatac tttgtttgct
     4141 ggaagtattg gtagaactga tttatatagt ggagacttta accaaatgga aaaatcactt
     4201 aagaaattat gcaaatatga agatgctgtt agagtatatc ctggacatgg accaaccagt
     4261 actctagggg ttgaaaagaa gacaaatcct tacatgtaat tttatattag tatttaaaaa
     4321 agtataaaat atttaataac tatattagtg agggggttct tctactgtta aatagaagga
     4381 gttaagttga tgttgtatgt ttttttaaaa ggacatgatt ataagtatga agtagcagaa
     4441 cttataaaat tgtttaccag tgaatttaag tttaaagact ctaatgctaa caataatata
     4501 gagtcaaagc atttaataaa tagattgatt tatgagaatg gtgtattatt ctcaagtact
     4561 gaatactatg aaaatggaaa tttaaagtac gagtcaatac agaatataga aaatatgaat
     4621 cttgaatttt tagatggatt tgatagtagc aaaacgattg aagaatttct taatagacta
     4681 gatagtgaaa gtaagaggaa ctttaaaaag ctgtgtaaag aaaatatcaa aaaaagcatg
     4741 tttgtagtgc ttaaacaagt atttaattct tatgtaccat ggggaatttt gactggtatt
     4801 agaccagtca aaattgtcca caatttaatg gataaaaagc tagatgataa tagcataaga
     4861 accattctaa aagataatta ttttataata gataagaaaa tagatttagc tcttgaaata
     4921 gctaaaagag agcgattatt tatgtatcct atagataaaa ataaaatttc tctttatgta
     4981 agtattccat tttgtccaac tagatgtgta tattgttcat ttccttcaaa ttcattaaaa
     5041 caatttggac atttaaaaag agaatatgtt tataaattga ttgaagaaat taaggggttt
     5101 gccaaagtta ttaaggagac taagaaagaa gttgaaactc tatatattgg tggtggaact
     5161 ccaactacac ttgatgagga agaattagat ttgcttataa attcattgtt caatgaatta
     5221 gatttaagta aaattaaaga atttactgta gaagcaggta gacctgacac tattacagaa
     5281 caaaaactaa gagtgttaaa aaaacacaat gttagtagaa ttagtataaa tcctcaaact
     5341 atgaatgatg ataccttggt aaagattgga agagagcata gagtatctga tttagtagat
     5401 tgctttaata tggctcgtag aatgggattt gataatataa atatggatat aattttaggt
     5461 cttatagatg aagaattaaa tatggtaaag aacacattgg aggagataaa aaaactttct
     5521 cctgaaagcc ttacagttca tacattagcc ataaagagag catccaactt aaatataaat
     5581 atggataggt ataaagaaca tctaacacaa tatgaagaaa tggtaaaaat gatagattta
     5641 tcaatgaaat atgcaaaaga tatgggatta aatccatatt atatgtacag acaaaaacat
     5701 atgttgggaa atttagaaaa tataggttat gctaaagaag gatatgagtg catttataat
     5761 atacagatta tggaagaaaa acaaagtaac tatgcattag gtgcaggtgc tatatcgaaa
     5821 tttgtttatg tagatgaaga tagaattgaa agagtagaaa atgtaaaaaa tgttgaacaa
     5881 tacattgaaa gagtagatga aatgataaaa aggaagaaag aagaggtata taaaaatgtt
     5941 gactaaagct cctagaggaa ctaaagacat aactccaaaa gaggcttaca aatggcgtta
     6001 tgtagaaaat aaatttagag aaatatgtgc tttatatgga tatgaagaaa tggtaacacc
     6061 tatatttgaa catacagaac ttttcaagag aagtgttgga gatactacag atatagttca
     6121 aaaagaaatg tattctttta aagataaagg tgatagagaa attaccttaa agccagaagg
     6181 tacagcaggt gtagtaagag catttataga aaataaatta tatgcagata ctcaaccaac
     6241 aaaattattt tatgtgacac cttgttttag atatgaaaga ccacaagctg gtagacaaag
     6301 acaatttcac caatttggaa tagaagcact tggaagtgat actccttcaa tggatgctga
     6361 aataatcgca ctagctgttc aattttttaa tgaagttgga ttaaatgatt tggtagtgag
     6421 tataaattca gttggctgtc cagtttgtag aaaagagtac aatgctttat taaaagagta
     6481 tcttgactca aaagctgata tattgtgtga tacttgcaat gagagaagag aaaaaaatcc
     6541 aatgagggtt atagactgta aaaatcctac atgtaaagaa aatatcaaag atataccatt
     6601 tattgcagac catttgtgtg atgattgtaa atctcacttt gataaacttc aagaatactt
     6661 aaaggaaatg aatataaact ttgtaattga taaaacaata gttagaggct tagattacta
     6721 tagaaaaact gcttttgaaa ttatatctaa tgatatagga gctcaaagta ctgtatgtgg
     6781 tggaggaaga tatgatggtc ttgttgaaca attaggagga ccaaaaggta taagtggaat
     6841 tgggtttgga ctaggaattg aaagactttt acttacactt gaaggtaatg gaattgaaat
     6901 agaaaaccct caatctacgg atatatttat agtaactata ggtgaagaag caaatacaag
     6961 aagctttaaa ttattaaaag acttgagaca aaatcatata agtgcagata aagaccatat
     7021 agagagaagt gttaaagcac aatttaaata ttcagataaa ataaattcaa aatttacaat
     7081 agtgattgga gatgatgagc ttaaaaatga cactgcgaca ttaaaaaata tgaaaacatc
     7141 agagcaaact actgtaaaat taagtacatt agttgaagaa ttaaagcaaa agctgtaaaa
     7201 taagaaagta tgatgttttg tgtattagat aaatatatag cattaaaatt aaataaaatg
     7261 gaggtagtat cttggaaact ttaaagggtt taaagagaac tcactactgt ggagagttga
     7321 gagaaaaaaa cataaatgaa gaagtcgtac ttatgggatg ggtacagaaa aaaagaaatt
     7381 taggtggtct tgtatttgtt gatttaagag atacaagtgg attatgtcaa atagtttttg
     7441 atacagatgt agataaagaa gcttttgaaa aagctgaaaa attaggggct gaatttgtaa
     7501 tagctgtaaa aggaaaagta tgcgaaagac aatctaaaaa ccctaatatg ccaactggtg
     7561 atatagaaat atttgcaact gaacttagac ttttaaacaa gtcagaaact ccgcctattt
     7621 atataaaaga tgatgatgat gtttctgaag cactaagatt aaaatataga tacttagatt
     7681 taagaaaacc atctatgcaa agaaatttaa aattaagaca taaagtaatg aatattacaa
     7741 gaaattactt atcaaacaat agattttgcg aaatagaaac accattttta atagctccaa
     7801 caccagaagg agcaagagat tatctagtac caagtagagt taatccagga aaattttacg
     7861 cattaccaca atcaccacaa ttatataaac aattattgat ggtaagtggt atggataggt
     7921 atttccaaat agttaaatgc tttagagatg aagatttaag agcagataga caaccagagt
     7981 ttacacaaat agactgtgaa atgtcttttg tcgagcaaga agatgttatg agtatgattg
     8041 aaggtttgct tgaagcgata tttaaagaag ttttagatgt agaattggca cttccacttc
     8101 ctaagatgac ttatgcagaa gctatgtcta aatatggttc agataaacca gatactagat
     8161 ttggatatga gttaacagat atatctgatg ttgtttgtaa ctgtggattt aaagtgtttg
     8221 ctgatgctac acaaccagga aaaagtgtta gaggtataaa cgtaaaaggc aaagctgacg
     8281 actttactag aaaacaaata tcatcactag aagaacatgc taagacatat agagcaaaag
     8341 gtcttgcttg gatgaaagta ggacaagaag gagtaacttc tccaatagca aaattcttta
     8401 atgaagaaga aatgaacgct atacttacaa aaatgaatgc tgaagtagga gatttacttt
     8461 tatttgtagc tgataaaaac tcaatagtat ttgatgcttt aggacaagtt agacttgaag
     8521 tagcaaatag gcttaatcta ttggataaaa atgtatataa cttactatgg gtaactgagt
     8581 tcccagtttt tgaagaagat gaagaaacag gcacattttc agcaatgcat cacccattta
     8641 catcacctat ggatgaagat ttagacaagt tagaagaagg tgataagtca tctttaagag
     8701 cgaaagctta tgatattgta ttgaatggtt atgaaatagg tggaggaagt gttagaatat
     8761 caaattctga tattcaatca agaatgttta aagcacttgg tttcacagag gaaagagcta
     8821 atgaaaagtt tggttattta ttagaagcat ttaaatatgg tactcctcct catgctggtc
     8881 tagcttttgg tcttgataga cttgttatgt tacttgcagg aactgataat ataagagaag
     8941 ttattgcatt ccctaaaaat caaaatgctg tttgccctat gacaaatgct cctacattgg
     9001 cggaagatga gcaattagaa gaattaagta taaaagttga tataaaagat aatgaataat
     9061 atgtagttgc aagaaactaa aatgtaaaaa gtctggatat aaagtatatt tatattcaga
     9121 ctttttttat tagaattatt ttatattttt tctctatata ttttcataaa ttatccttgt
     9181 attctcagcc aatataatta aaatactatg tttaaaaatt ttacataaac ttgattgttt
     9241 tagaaattac ttctgtaatg agtacacgat tttaattaaa gaatatataa caaatttgaa
     9301 ataagtctaa tatatgactt aatagattaa attaaaataa taaatttgga ctatttatct
     9361 atttataact taaattatat caaattgata acaaactaga tattatactt ttttgtagaa
     9421 tagaattaca attatataaa gaaaaattct attaatagtg aaaaaaaagt agaaaaaagt
     9481 agaaattatt cttttataaa gaaagggagg gataatattg caagagagag aagaattagt
     9541 tttaaatcca gttccaaaag aggaaagagt tggatggtta gctccattgt ttaatatgct
     9601 tggttctaac atagctatat ctgaactaat ggttggagga actttaattt taggaatgac
     9661 tctttctaat atgattatta catcaattat agggaactta atattagttg caatcattat
     9721 gatacaaggt tatataggtt acaaagaagg gttaaatacg tatgtattag ctaagggggc
     9781 atttggagag ataggaggaa agtatcttat atctctatta cttggtataa ctagttttgg
     9841 atggtttggt gtacaagcag gggttgcagg actatcagta caaaagatat tcccaagtgt
     9901 aaacctaaca ttagttacag ttatacttgg attatttatg gttgtttttg cactgtatgg
     9961 ctttaaagct atggctaaat ttaattactt agttatacca ccacttatga tattaatggt
    10021 gtggggagtt tttaaagctt tttctactta tggagttgaa gctatttata attatactcc
    10081 tcaaacaact atgagtatgg ttgaaggtct taatattgta gtaggtcttg ttatagttgg
    10141 tgcaattata tcacctgacc agttaagata tacgagacga gtaaaagata tttggattat
    10201 cagtttttta ggtctaggga ttatttcatt attccaacaa gttgcagctg gtgtaatgtc
    10261 tatgggagca cctacttggg atataacaga agtattggct aatttaggtt ttggatgggt
    10321 agcgtttgtt attttaatac ttgcatcttg gtcaacaaac gtatcaaatg cttattctgg
    10381 tggattagca ttaaaaacta tttttccaaa tgtaaaaaga aatgttttga ctttagttgc
    10441 aggtctaata ggtacaataa tagcagctac tggtataata tttaaatttc agtcattttt
    10501 atctttcttg ggaatcgcag ttcctgcaat agcaggaatt atgtggtgtg agtattattt
    10561 tatacaagga agaacttata agcatagaga aggaataaat tggattgcag taataagctg
    10621 gttaataggt tttgcagcgt cgtattatag ttcaaaaata aatttcttaa tacctccgat
    10681 aaatggaatt gtagtgagta tggttattta ttatatatta atgaaatgtt ttggaatcaa
    10741 agataaataa agggggcaat tacatgggtc gtaaggtgaa aataggtata attcaacaac
    10801 acagtgtact tggagatgtt aaaaaaaata tagaaaaagc agtagagatg atagatgact
    10861 taggaaaaca tggggcagat ataatttgtt taccagaact atttgctaca ggatataatt
    10921 tagagtcttt aggtggagtt aaaacattag aattaataag agaacataat aagtacatag
    10981 aggaatcaat gtcagaagct gctaaaagaa ataatgtata cctgatatct ccatatggaa
    11041 ccttagaaaa agggtctaca catgtttata attcagcagt tatatttgat agaaaaggta
    11101 agattatggg agaatactgt aaaaatcatc tgtggtcatt agaagcagta tactttaaag
    11161 gaggggaaaa agtagaggta tatgatgctg attttggtag atttggtgta atgatatgtt
    11221 atgatgcagg ttttccagaa gtatccagag aattgacatt aaaaggttct gaaataatat
    11281 ttataccatc tgcatggaga atacaagatg aagatatgtg ggatttaaat gtatctcaaa
    11341 gagctttaga gaacactgta tatacagtag gtgtaaattt agtaagtaat gactctaatt
    11401 taatactatt tggaaagagt aaaatatgca atcctagagg tactgtaata actcaattag
    11461 atacatacag ggaaaaatat gtattatcag agattgattt agatgaaata aaaaagtata
    11521 gggaagatat accctatttg aaagacagaa aaagtagcta taacatataa ttctatacta
    11581 taatacaagt tcaatataaa atgataaaaa gaggatgtat caatattaag attaaattta
    11641 ttatattttg atatatcctc tttaatatta tcaactttga tttaaatatt tttaaaatag
    11701 ttgataagaa agtttttgaa caacaacaca gctttagatt catagttttc gctaaaccat
    11761 gaaagattta aatatctttt aaattcaata tctttgatat gtaacaactt aatctcaggg
    11821 tcttgagata atccccatga ttttccacat ataaatccaa ctccttgaag tgattttata
    11881 agagcataaa ttgtatatgg tgaatcagac tcaaatgcta ttttgggttt gaaattagct
    11941 gattcgcaca ggatatcaat tacctctcta taattctcac ccttcgttat aactataaag
    12001 ttttcatctg aaacttcgct tagatatact tcatccttta atgataaagg atggtttatt
    12061 gaaactccta atagtatttc ttcgcatgtt aaagttatgc tattttctga gtttagcttt
    12121 gtaaatgaag aagatatgta caaatcaaaa tcactttttt tataagatga tggcaaagta
    12181 tgtgacacat taaaagtaat attgggatat agttttctga aattactcaa aagtgatggt
    12241 aacacattag ccgctgcagg agataatatc tttacttctc ctgaattttc taaagaagtg
    12301 tcttgtaact ccttttttga actttctact aaatctaaaa tgctatctac tctttttaga
    12361 aacaatcttc catactcatt taaaacaata tatcttcctt ttctgtcaaa taactgaact
    12421 ccaagttctt tttctaagga agatatagtt ttgcttaaag ctggctgtgc tatatttaac
    12481 ttgtgtgatg cttttgttat gtgctccatt ctagcaacag tttgaaaata cttaagatgc
    12541 aataaatcca tagtataaaa tacacctctt ttaatatgac ttgagttata tcaattttat
    12601 aatcaactcc acattataca ttataatata aagttta
//
LOCUS       DAMJVX010000105        12353 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMJVX010000105 DAMJVX010000000
VERSION     DAMJVX010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12353
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.109"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            81..284
                     /locus_tag="PC407_002510"
     CDS             81..284
                     /locus_tag="PC407_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2497948.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            448..1602
                     /locus_tag="PC407_002511"
     CDS             448..1602
                     /locus_tag="PC407_002511"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDF2497949.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELVNITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFRVEQENIDVVVHPQSIIHSMVQYADSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            1615..2619
                     /gene="rseP"
                     /locus_tag="PC407_002512"
     CDS             1615..2619
                     /gene="rseP"
                     /locus_tag="PC407_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDF2497950.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSNKVVNVTPKNNNGKYEIGIQPQREKDFLASIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            2802..3857
                     /gene="ispG"
                     /locus_tag="PC407_002513"
                     /gene_synonym="gcpE"
     CDS             2802..3857
                     /gene="ispG"
                     /locus_tag="PC407_002513"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDF2497951.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSRERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKHGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            4205..5029
                     /locus_tag="PC407_002514"
     CDS             4205..5029
                     /locus_tag="PC407_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497952.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLASTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSIWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(5502..6625)
                     /locus_tag="PC407_002515"
                     /pseudo
     CDS             complement(5502..6625)
                     /locus_tag="PC407_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
     gene            6822..7046
                     /locus_tag="PC407_002516"
     CDS             6822..7046
                     /locus_tag="PC407_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497953.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            7099..7623
                     /locus_tag="PC407_002517"
     CDS             7099..7623
                     /locus_tag="PC407_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2497954.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(7728..7874)
                     /locus_tag="PC407_002518"
     CDS             complement(7728..7874)
                     /locus_tag="PC407_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497955.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(8013..8939)
                     /locus_tag="PC407_002519"
     CDS             complement(8013..8939)
                     /locus_tag="PC407_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2497956.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            9130..11001
                     /locus_tag="PC407_002520"
     CDS             9130..11001
                     /locus_tag="PC407_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDF2497957.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNASCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(11093..12028)
                     /locus_tag="PC407_002521"
     CDS             complement(11093..12028)
                     /locus_tag="PC407_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDF2497958.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
BASE COUNT         4870 a         1298 c         2086 g         4099 t
ORIGIN      
        1 taaataatta tttatctaaa tagatgaaaa ttttccaaaa tagataaaat gtgatagaat
       61 atattttgcg aggtgataac atgatttcat acgctccatt atggaaattg ttaattgaca
      121 gaaaaataaa gaaaatggaa tttgtaaata tatcaggtat aagtatttct gttttaggca
      181 gactaggaaa tgataaatct gtttctatgg atacaatgga gaagatatgt ttagcactag
      241 actgtaaaat agaagatgtt gtggaaataa aaaagggcat ttaataatac tagaatattt
      301 tataatattc cacaagatac aaaaaataat agattaattt taatctgtgg aataatatat
      361 gaaatttcta tgaatagtaa aaataattgt tttagtagtg ttataatgtt ttgacagggt
      421 atatttatta ttagaaagga ttattatatg aaaaagatat caattttagg ttctacaggc
      481 tctataggta aacaaacttt ggatgtggtt agagaacata gagataagtt tgagatagtt
      541 gcaatatcag ctaacagtaa tattgaatta ttgctagaac aaatagtaga gtttaaacct
      601 aaatatgtaa cagtttttga ggaaaataag gcattgaaat taaaagaaat gttgcctaaa
      661 aatatagaaa tagaagtttt agcaggaatg gaaggattaa aaataatatc atcacttgat
      721 gaagtggatg tccttttaac tgctgtagta ggtatgatag gattagtccc aactctttgt
      781 gctataaaga agggaataga catagcatta gcgaataaag aaacgttggt aactgctggt
      841 gaacttgtaa tgaaagaagc agaaaaatac aatgtaaata ttcttcctgt tgatagtgaa
      901 catagtgcta tatttcaatg tctaaatgga gaaaataaaa aaaatataga aaagataata
      961 cttacagcat ctggtggtcc atttagagga aagaaaaagg gagaacttgt aaatataact
     1021 aaaaatgaag ctttgaagca tccaaattgg agtatgggaa gaaaaataag tattgattct
     1081 tcaacgctta tgaataaagg acttgaagtg atagaagcta gatggttgtt tagggtagaa
     1141 caagaaaata tagatgtagt agttcaccca caaagcataa ttcattcaat ggtgcagtat
     1201 gcagatagct caataattgc tcaattaggg tgtccagata tgagactacc aatacagtat
     1261 gctcttacat atccagatag aatggaaagt agctttgaaa gaatgaattt ttcaaaattt
     1321 agtactttaa cttttgaaga accagattta gaaacttttc catgtttaaa attggcctat
     1381 gagtgtttaa aaatgggagg aacttattct tctgttttaa attcagcaaa tgaagtttta
     1441 gtaagtgaat ttttagagga taaaattggt ttttatgaca taccatatta catagagaag
     1501 actttagaag ttcatagtag cataagcgaa ccaacattag aagaaatctt agaaacagat
     1561 agatggagta gagcttatgt tgcaaatctg ataaaaaaat aggaaggtga atttatgact
     1621 ataatagccg cattaatact atttagtata attgtattaa tacatgagtt aggacatttt
     1681 atatttgcaa aaagaagtgg tatcaaagta aatgagtttt ctataggtat ggggcctaag
     1741 atatatagtg taaaaaaaga tacagagtat tctataagag cacttccaat tggtggatat
     1801 gtcagcatgg aaggtgaaga tgaagaacaa ataagtccaa attcttttgg aaacaagtct
     1861 atattacaaa gatttagtac tattgtagca ggaccaatat tcaatataat attagcagca
     1921 atacttttag ttccagtatt tttgtatata ggttctccaa cgactaaatt aggaaagata
     1981 atgccagata ctcctgcaca ggctgtaggg cttcaagtag gagataagat aaataaaatt
     2041 aatggtaatt ctgttaagac ttgggatgaa gttgcaaata taataaacac ttcttctggt
     2101 ggagaattaa aacttagtat aactagagat ggaagtaata aagttgtaaa tgtaacacct
     2161 aaaaataaca atggaaagta tgaaataggg attcaaccac aaagagaaaa agactttttg
     2221 gcgtcaatag taaatgcatg taaaactact gtagatatga caaaacagat gttaacattt
     2281 ctaggtcaaa tgattacagg acgtgtacca ggagggattg gcaatgctgt agcaggacca
     2341 gtaggtgtta ttggtatggt atcagatgct gctcgtacag gcatcataaa tgtagtgtat
     2401 ttagcagctg tcataagctt aaatttaggt atagtaaatc tattaccaat accagcactt
     2461 gatggatgga gaatacttat gctattacta gaagctgtta gaggtggtaa aaaactcgac
     2521 ccaaacaaag aaggcatgat aaatgttgtt ggttttggag ctttaatgtt atttatgctt
     2581 tttatcacat ataaagatat attaagatta tttcaatgat ttagtttttt agagatttta
     2641 agaaaatagg ctatgtagtt ttaaataaga ataaattaat ttttatgtat gaaatttatt
     2701 cttatttaac tagatagctt tttaatttat aatatacatg taggttataa tattttacta
     2761 tttaaagaga ttcaatataa aataatatat agaggtggaa tatgagttac gagagaagaa
     2821 agacgagaga agtaagtgta ggaactgtaa aaataggagg agaaaatcct ataagtatac
     2881 aatctatgac aaatactgat acaagagatg cagatgctac aatagctcaa ataaagagac
     2941 tagaggaagc tggttgtgat atagttagag ttgctgttcc tgatattaaa gctgctaaga
     3001 atatagcaaa gataaagtct agtgtaaata taccaattat tgcagatata catttcgatt
     3061 ataagctagc ccttgaagct atagaacaag gtgtggatgg aataagaata aatcctggta
     3121 atattggaag tagagagaga gttaagatgg tagttgagaa atgtaaggaa agaaatttaa
     3181 aaattagaat tggagtcaat ggaggttctt tagagaaaga acttttaaaa aagcatggct
     3241 ctgcaactgc tgaagcatta gttgaaagtg caatgggaca tattaagatt cttgaagact
     3301 tagattttca taatatagtt atatctctaa aatcatccga tatttataaa actgttgatg
     3361 catatgaatt aatatcaaaa aaagttgatt atcctcttca tattggaatt acagaatctg
     3421 gaagtgttca caaaggaaca ataaagtctt ctataggggt aggagcactt cttcttaaag
     3481 gaattggaga tactgttaga atatctttaa caggcgaccc tgttgaagag gttattgttg
     3541 gtaaacagat tttaagaagt ttgggacttt taaatgataa gattaaagtt atatcatgcc
     3601 ctacatgtgg tagatgtaat atagacttaa ttagtgttgt aaatgaggtt gaagaaaaga
     3661 ttggtagtat ggaaaaaaat atcactgttg caattatggg ctgtgctgta aatggacctg
     3721 gtgaagccag agaagcagat ataggtattg ctgggggtaa aggtgaaggt cttttattta
     3781 aaaagggtga aatagttaga atgattgatg gtaacaaatt agttgatgag ttgctagaag
     3841 agattgaaaa attatagtat acaattcata tatatattaa ttgactagat ttttaaatac
     3901 ttttgtaata agtttttatt tacagtattt aattatctag tcatttttta ttttataaac
     3961 ttcattaaat gtaagaaact tatattctaa tttgactata tatgtatgag ttaaatcata
     4021 ttgtaaattt ataaaattaa aaaaagattt aatatagtga aaatttccat caatagtatt
     4081 gaaacttgat agtaactatg ataggatata gttactagaa cagttggtaa attatgttaa
     4141 catagatttt aatgttttgg taaattgttt tattataagt taatagaaaa agaggtgaag
     4201 aactgtgaga tataaaaaaa tatctagctt acctatttta gttgttgctt tgctgattac
     4261 tggaacgaca acaacatttt ctcttagtac gaccaattca gaaaaaacaa atgtatactt
     4321 agcaagtacg gtaagtactg tcacaaagaa agtatccgat attcaaagta gtaaaggtct
     4381 tgtttataat gagaatattc cattatttat aaataatagc aaaatacagt atgatgatac
     4441 tccatatcat tggcctagca atgttataag cttaacaaat agcagtgaaa aggcaattat
     4501 ggattatgag attacatgct tagcatatga taaaaatgga aaaccacttg agctatattg
     4561 ggatgctcaa aatgtggcag cagatggcga agtaggaagt gttggattta gcccagctgg
     4621 tgtagactat ggtattgtta caggcatatc tcctgtgtct ccaaaatcat attcacatac
     4681 ttatagaaaa atgcaacaat caccaccaca ggatataata agtatgtttg aaaaacaaca
     4741 aggcaaggca tgggtagaaa attggttgaa agaatggaaa caaatggaga aagaatatgc
     4801 aaagcaaaat gcaatagcac caggtaagaa tcaaaatgat gcctttctat tgtttgataa
     4861 atggaaacaa tcaactggag agcatggtgt aaagtacatt atatcttgtg taaaacaagt
     4921 aacatttaat gatggtagta tatggaaaaa ttcagcatat gagaattggt tgaaaagttt
     4981 tcaagggaag gaagtttcaa atagtgtatt agaaaattac tataagtaat gtttaatgat
     5041 agcaaaatga gtacgaattt aattaatgtt aatttaatta aataaggact attttgttta
     5101 aaaatatcag tgttacaagg aaatttttat tgtgacaaaa tggtatatat tcaaaataaa
     5161 aaagcatgtg tcaagttttg atacatgctt ttttattttg aatatatgtt ttctataaat
     5221 ttatgatttt ttagttatgt aattttatat gtgaattttt agatttttat atagatagtg
     5281 aaataatgtg gaagttttat gttttaaaaa ttttagttaa aacatttatt aaaaaagata
     5341 atagaaatgt tttaactaaa atagttagaa ttgaaatgtt atacagatag tataagtact
     5401 atcaaattat ataagattgc ttgaagttaa ataatttatt taaatagaaa acagctgatt
     5461 taatatcagc tgtatatgaa taaaattttg aattgtagtg tttagttata tggatataat
     5521 tcataataag ttagcaataa atcaaccatt ttaccataac tctgagtacc ttcagtaacg
     5581 ccattagctt ttaagtaaga attgttaaac tcattagaaa tttcattaat tttaccttca
     5641 tatttttgcc aaaattcact ttcatttttt aaatctctac ggacagaatc tgaaatgcca
     5701 gcagaaatat caacatatgc attataatca accttactta aagcagaagc agtataattt
     5761 aaagctaaaa tatatccaga atagttaaaa tcaatatggg ggtgttttat agaagtaaga
     5821 tacgctataa aattagcctc atcttcacta gcaaatcccc tttgatgtgc tatttcatgt
     5881 tcaactgtac aaggaatata taagtcaggt attgctatat ttacatttgc ctcacctgta
     5941 aaaggaaagt atataccagt tattcctgta tagcacatta gatttgatga aataatatac
     6001 ttaggcttag aataactacc actaacacta ggtaatatat ccaagatatt atcgtatcct
     6061 agttgagctc tatttatgac accattataa tctgtattag attttacaat accattttta
     6121 tcttgtaatg tgagttttct agtttcattt gctttaatta caaggaattt atataattta
     6181 gtaagctctt taacactata ttgttttgat tggatagagg tattgttttt taaattatag
     6241 ttatttataa gtgtggtttc tagtggaatc ctattatagt ttattcccca aagcactaca
     6301 aaaagaaagt agactattga tacaatggat aatatattta aaatggaatt ttttaaaaat
     6361 atgtttaatt ttttttctta ttaaaaattg tagataaagt acaacataaa aataagaaga
     6421 tagatataac tattatgtac attgtaatct catagagaga aaatgggaaa attccagaaa
     6481 ctttactcaa tatttgtaca attattttat ctatgaattg ggagtaatat ttttcaacaa
     6541 tatttggaac tttgctggct ataaaattta aaagtaaagc aataggaaag agaattaaag
     6601 aaaaatattt tgtttttttg ctcatatttc acctcacata cattagttta tataaacata
     6661 ttatatatca attgaagaaa gattgtaaaa taagtataaa tatatctatt tataacttta
     6721 tactaaataa atgataagtt ataatatatt aatatgaata agcagtatat atacaactaa
     6781 taataagaaa aatatcaatt atataaggag tagataaaaa tatgataata aaacttacta
     6841 aagacaattt taattttaga tttaaacaat atgaaggtat gttttttcat gctgaagtag
     6901 ttgaaggtat ggaatccatt agatataaaa taactaagga taaagaaggt acagaattaa
     6961 aagaatttgg aagtacacat ataaaagata atatgatata tgtacctcag gtagaagcat
     7021 acatagattt gaacagtata aaataaagat gtaaaatttt aattaaaaat aaagattatg
     7081 tttgaggggg aatccaatat gagtaagcct atagatataa caataagacc tataagaaga
     7141 gaagatgcac aagcttttaa tgaaattaga agaacataca gtgttatgag aaatactttg
     7201 gcattaatga gtgacagagt cgataaaact gaagctatgt tagcttcttt gggagaaaat
     7261 gattatatgt ttgtagctga aactaataat aatggagaaa aagatgtaat aggttttgct
     7321 gggttacatg taaattcttc tccacgattg agacatagtg cagaaatagg aataagtgtt
     7381 gcagagaatt atcagggaag aggagtaggt aagaagctta tggaacaact tttagatata
     7441 gcagataatt ggattatgtt aactagagta ggattagaag taattgtaga taatgaaaag
     7501 ggattaaatt tatataaaaa actaggtttt gaaatagagg gaactaaaaa atatgctgta
     7561 atcagagatg gaaaatttga agatgtttat attatgggaa gatataataa aaatttaatt
     7621 taaagaagct aatagagaag taaaatagag tgtctttaat aaaaaattta cattaaaaat
     7681 aatctatttt acttctctat tgtattaaaa aacttatttt taaatgttta caaaaacatt
     7741 ctatatacaa ataaaattat tagcaatatc aacgctattt taaaagctaa actagcaaca
     7801 gttttaatga tttttacaga aacacctata gcaactatag caaatactat tttaaagagt
     7861 aaatccatat ccatatttaa gcctcctatt ctataataca tatagatatt attgacaaat
     7921 atctaggaaa aaattccatc aatatactac aataaaatgt ttatatttgt aatttattga
     7981 atatgttatt atataataca taaaaaaata aatcaagata agagtaaaat acttataaaa
     8041 ggtatagata taactgaaaa tagtgttgta aaaaacacac attgtgaagc tagtgttata
     8101 ttggagtcat attgattagc cattatagca gtatttgcag cagcaggcat tgaagaaatt
     8161 actactggta tagctaaaat catcttatca tttatccatc cttttagaat aaaatacaca
     8221 atcacaggaa gtaccaataa tcttataaaa gtgactatat ataatttttt gttgacaaaa
     8281 caatcgagag ctgaagaatt agcaagtaag cttccaatga taatcatgga tattggtgta
     8341 gttatacttc ccaacatctc aagtggctcg tttataaatt ggggtaacct aagtcctgtt
     8401 acaaataaaa aaagtccaat aacaatagct attgtagcag ggcttataag tgattttatt
     8461 gaaaaacctc tacttgtact gcctttagat aaaagataaa ttcctaaagt gaaagaaaat
     8521 aagttaaaag gcaaattgaa tattgcagta tagaatattg cttcatgacc taaaactgct
     8581 tcaattacag gataacccat aaaagcaaca tttgaaaaaa caactatata ctgatatatt
     8641 cccaaatctt ttttatcatt acattttaaa atgtatttca ataaaaatgc aattataatt
     8701 gaaactatat acataatcaa agatgtaaat aataacaata gtattttttg tatcatttta
     8761 ctatcaaagt taatttgcat ggagctaatt atcattgatg gtaaaaaaac tgtcattgtc
     8821 aaagttgaaa gcttagatgt gcagtggtca tctaataagt tgaattttct gacaaaatat
     8881 ccaactaaaa taattataaa taaaactgca acttggataa aaatattgct gaaattcata
     8941 aaatgtcccc ccctatatat acattatata ccaattacat aataaaatct aatgattata
     9001 aaatttaaaa tttaatgtta caatatagtg acaaaaggta aaaattcaat attatagcat
     9061 taattataga ttataatgta taataatgtt tgagagaata tattttataa aatgggtagg
     9121 tagcgaataa tgaaaaatac aaaattttct ataaaaaaaa taaaaaatat atctaatgac
     9181 ccgaaagtcg tacatactaa gggtgccctc cttgaaaaga tgggtaagat ggaaagcgca
     9241 atagagttgt ataagagtgc tgcattggat aattacgtaa aatctcaata tactttagga
     9301 aatatatatg aaagtaaaaa acaatttaaa gaagcagaaa agtggtattc aatggcatat
     9361 aaaagtggaa gtgaagatgc tgcttttgat ttaggtaata tgtactataa gttagatggc
     9421 tatgaatatg ctatatattg gtatgaaaag atagcaaatt taggttattt acaagcacaa
     9481 aataatttag gtgtatgcca ttttaaaatg aaagattttg taaggtgtga aaaatggtta
     9541 aaaacagcag cagacaagaa tttaggtaaa gcttgtttta atttaggggt attatatact
     9601 tttttggaga aagatgatga agcatatgaa tactataaaa aaggttctgt gcttttagat
     9661 gatgatgcaa aatataattt agcaatatta aatggaaaaa agaaagataa taaatcaaca
     9721 gtaagtttat ataaacagtt gtacaagtca ggtcatatga aaggttgctt taacttgggt
     9781 atgattatgg aaataaatga taatttagaa gatgcagaga agtattataa aaagagtgct
     9841 gatagagatg atgtgaagtc tgagtatagg cttgcatatg tatatgatag aaaagaagag
     9901 ctgggagatg caatttatta ttatgagaaa gcaatagaaa aaaatcatac actttctaag
     9961 tatagacttg ctaatttata taatagagaa aatgagatag agaaggcaaa aacttattat
    10021 aaaatggctg ctgaagatga tataacagaa gcaaaaaata atctggcagg tatatatttt
    10081 gaagaaaaag attatgaaaa tgctattaaa tattatgagg atgctatagc tgttggatgc
    10141 aaaagttccc ttgaaaatct tggggattta tattatcaga atcaagatat agaaaaagcg
    10201 atttcgtatt attcgaggat tccaaataat gcatcttgtc aaatcaaatt aggaaatatc
    10261 tatgaagatt taaataatat tgaagaagcc ataagttggt ataagaaagc atctgaaaat
    10321 ggagatacga ggtctagtta tagattgggg tgtatatatg agagcttagg aaatacaaaa
    10381 aatgctagaa agtattttga aatggcaagt agcaaaaatc atatgaatgc tcgtatacat
    10441 ctaggtagaa tttattttag agaaggtaaa ttggaagagt ctaaaatgat gtttgataca
    10501 cctgccaatg aaaataatgt gtacgctcaa catatggtag gattaattta tgatatgttc
    10561 tataaagatt atgtaaattc taaattttgg tatgaaaagg ctagagcaca aggttgtgtt
    10621 gaatcaatat ataatttagg ccaaatttat ttaaaattaa atgatgacgc agaagctgag
    10681 aaatattata aagaaggtat taaatatgga agtaagaagt gtgagtatat gcttgcaggt
    10741 ctttattata aaaagagcct agatatgtac atttctttag caaatgaaga gtacgataat
    10801 tgtgaagaga tagtagaagg ctttcccaat ttaaatatag actttgatga agtattaatt
    10861 cctccattta agttgcaaga agaggtaata gatgaagaag agtatgtacc aatttatata
    10921 ctaaatataa aagaaagttt agattcaatg ctagaagggc ttgaaactga aatgattata
    10981 gatgaagaac atgatagtta aaaagtatta taagattata tagatttaaa tatataaaag
    11041 ccattaggat aaatgtaaat caattaattc taatggcttt tttattaata ttttataata
    11101 gcttatttct tgacatgaaa aatgtaatca ctatacatat tatagaggta ataagaagta
    11161 ttatccaaaa tccgtttggg ttattagaaa atggcatgtt gatgacattc ataccaaaga
    11221 atcctgatat tattgtaggt attgagaaaa ttagtgtaac tactgttaaa atttgcataa
    11281 cattattctg attattactt ataattgcac tgaaagcatc cataattcta ctcaatatat
    11341 ctccatatat tttagccata tcaagagcct gtctattttc aactataaca tcttctaata
    11401 ggtcttcatc atctggatac ttctttatac cagcagttct aaccattttg tttaaaacaa
    11461 gttcattaga ttttagtgag gtagagaagt ataccaaaga tttttctaat tccaataatt
    11521 gaaccaattc tttgtttttc attgatttgt aaatttctcg ctctattatg attgtcattt
    11581 tatttatttt tcttaagtaa tgcaagtaat aagctgcatt tttgtgaaga atttgaagca
    11641 aaaagcgagt ctttttaaac gtaaaaaaat ctttaatatg accaactata aagtcattta
    11701 aaatcttagt ctgtgcatca caaactgtta caatagcttc ttcagttagt ataataccta
    11761 agggtattgt agtataatga gaagaattag agtcactttc gtctactggt atatctataa
    11821 gtataagagt atgattgtct tcaacatcta ttcttgagcg ttcttcttca tctagtgcag
    11881 attctatgct ttctacatct atatttaata aattagaaat ttcgttgatt tcactatgat
    11941 taggttcaac aagatttatc caacagccat cttcaaaaaa actaagtttc tctaattttg
    12001 aatctatagt tttgtaatat cttatcatgg caacacatcc tttattcagt tttatataaa
    12061 tctcatcatt taatagtatc tattaattaa ctaatttata taactgaata tatgtattag
    12121 ttttgatttt agatattatt aagtgatgaa aatgtaaaaa attctctccg caacggacta
    12181 gtatccattg cccatcactc tccttattta ttattttata tatagctatt tatgaaatgc
    12241 gtattttaag cactctttaa tataatacct tttccaggta tataaatcaa gttttgattt
    12301 gattaaataa tatttttacc ataatttaca cataaagtat ataagttata ttt
//
LOCUS       DAMJVX010000106        12154 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMJVX010000106 DAMJVX010000000
VERSION     DAMJVX010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12154
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.110"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            161..949
                     /locus_tag="PC407_002522"
     CDS             161..949
                     /locus_tag="PC407_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2497959.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            1420..5136
                     /locus_tag="PC407_002523"
     CDS             1420..5136
                     /locus_tag="PC407_002523"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDF2497960.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGQSFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     VEATEDEDIENLEEFTEDDLFEEEIDFESDDFDM"
     gene            5178..8663
                     /gene="rpoC"
                     /locus_tag="PC407_002524"
     CDS             5178..8663
                     /gene="rpoC"
                     /locus_tag="PC407_002524"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDF2497961.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVLVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            8952..9374
                     /gene="rpsL"
                     /locus_tag="PC407_002525"
     CDS             8952..9374
                     /gene="rpsL"
                     /locus_tag="PC407_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDF2497962.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            9497..9967
                     /gene="rpsG"
                     /locus_tag="PC407_002526"
     CDS             9497..9967
                     /gene="rpsG"
                     /locus_tag="PC407_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDF2497963.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            10016..12082
                     /gene="fusA"
                     /locus_tag="PC407_002527"
     CDS             10016..12082
                     /gene="fusA"
                     /locus_tag="PC407_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2497964.1"
                     /translation="MARKFPLEKTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFINKMDILGADFYNVISMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         4703 a         1536 c         2495 g         3420 t
ORIGIN      
        1 atgtaaaatt ttacatttta gtttaaaaaa atacacgagt gttttataaa taaaatgtaa
       61 taaataatta attaacaaat ataaatttta aaataagtta gatatttaga aaaatcgaaa
      121 tttgtaggat ttaaaggaca tattcaagga ggagttaata atggaaaaat tacaaggaaa
      181 aattgcagta gttactgcag caacaaaagg tattggatta gcatcagcag agatattagc
      241 aaaaaatgga gcaactgtgt acttagcagc tcgttcagaa gaattagcta atgaagttat
      301 aaataaaata agtgcagaag gtggttgtgc taagtttgtt tactttaatg ctcgtgaaga
      361 agagaccttt acctcaatga tagaagaggt agttaaaaaa gaaggtaaga tagatatact
      421 tgtaaacaat tttggttcaa caaacccttc tcttgacaaa gaccttgtaa ctggggatac
      481 agataatttc tttgacacag taaatactaa tttaaaaagt gtgtatttac catgtaaagc
      541 agcaattcct catatgataa agaatggaaa aggaagtata gtaaatatat caagtatagg
      601 ttcagtatta cctgatttat ctagaatagc ttactgtgta tcaaaagcag caatcaactc
      661 attaactcaa aacatagcta cacaatatgc taaagacaat gttagatgta atgctgtact
      721 tccagggctt attgcaacta aagcagcatt agacaatatg tctccagagt tcataaaaga
      781 atttttaaag catgttcctt taaatcgtat aggggaacca gatgatatag caaaagctgt
      841 tctattctat gctagtgatg actcatcatt tataacaggg gatttacttg aagttgcagg
      901 aggatttggt ttacctactc cacaatttgc agataatata ttaggataat taaattacta
      961 atatgcaatg aaagcttatg cattagttat agtagaaatg atatttttat aaaggccata
     1021 gatataattt atattggaaa ggtttgaggt tcaaaattcc aagttaaatt tatttatggt
     1081 ctttattttg tatattttaa taaattgtat atatttgtat aaatgtaatt atttacaatc
     1141 attttaatat ggaattataa ttaaataggg gggatggtac ttgtataaaa ttttttatag
     1201 aaaattagac ctataaatga aaaatatgga ttttatattg gaaaattgta cataatacat
     1261 aatatatgct taaaataatt tacaaaaaat caaaaataat gcatagttta catataagat
     1321 aaaaaatata ttgaaaaata cttgacatta tgctaaaatt attagatgcg ttagaatgaa
     1381 ctattgtttt atttttaatt attgagaggt gaaaagtgaa tgccacatcc tgtcacgata
     1441 ggtaaaagaa ctagaatgag cttttctaaa attaaggaaa tagctgatgt gccaaatctt
     1501 atagaaattc aagtagattc ctatgagtgg tttttaaaag aaggtttaaa agaagtgttt
     1561 gatgatattt caccaataga agattatact ggtaatctta tattggaatt tgtcgactat
     1621 tctctagatg ataaaccaaa gtacgatata gaagagtgta aagagagaga tgctacatat
     1681 tgtgcacctc taaaggttaa ggtaagactt ataaataaag aaacaggtga gataaaagaa
     1741 caagaagtgt ttatgggtga cttcccttta atgacagaaa gaggaacttt cgttataaat
     1801 ggagcagaaa gagttatagt aagtcaatta gttagatctc ctggtgttta ctatgcggaa
     1861 gaaagagata aaacaggtaa gagattgatt tcatctactg taattcctaa tagaggagca
     1921 tggttagaat atgaaacaga ttctaatgat gtaatatctg taagagttga tagaacaaga
     1981 aaacaaccag ttacagtttt acttagagct ttaggaatag gaacagatgc agagataatt
     2041 gaccttttag gagaagatga gagattatct gctacattag aaaaagataa tactaagaca
     2101 gtagaagaag gtcttgtaga gatatacaaa aaattaagac caggtgaacc tcctactgta
     2161 gaaagtgcat catctttatt aaatgcttta ttctttgacc caaaaagata tgacctagca
     2221 aaagttggta gatataaatt caataagaaa cttgctttat gttatagaat aatgaataaa
     2281 atttcagcag aagacataat aaatccagaa actggagaag tatttgtaaa agctggagaa
     2341 aaaataagct atgatttggc taaagccata caaaatgcag gtataaatgt agtaaatcta
     2401 ctaatggatg atgataaaaa agttagagtt ataggaaata attttgtgga tattaagtct
     2461 cacattgatt ttgacataga tgatttaaac ataaaagaaa aagtacatta tccaacttta
     2521 aaagaaattt tagatggata tagtgatgaa gaagaaataa aagaggctat aaaatcaaga
     2581 attaaagaac ttataccaaa acatatatta ttagatgata taatagcttc aataagttat
     2641 gagttcaata tattctataa tataggaaat attgatgata tagatcacct aggaaacaga
     2701 agaataagat ctgtaggtga attgttacaa aatcaagtta gaattggtct ttcaagaatg
     2761 gaaagagtta taaaagaaag aatgacggtt caagatatgg aagctataac gcctcaagcg
     2821 ttagttaaca taagaccagt ttcagctgca ataaaagaat tttttggtag ttctcagtta
     2881 tctcagttca tggatcaaac aaatcctcta tctgagttaa cgcataagag aagattatca
     2941 gcccttgggc ctggaggtct ttcaagagaa agagctggat tcgaagtgcg tgacgttcac
     3001 cattcacact atggtagaat gtgtccaata gagactccag agggaccaaa tatcggtctt
     3061 ataaactctc taggaactta tgcaaaaata aatgaatttg gatttataga atcaccatat
     3121 agaaaatttg ataaagaaac atcaacagtt actgatgaaa tacattattt aactgctgac
     3181 gaagaagatt tattcgtaag agctcaggca aatgagccat taacagaaga tggtaaattc
     3241 gtaaaccata gagttgtttg tagaactgta aatggtgctg ttgaaatggt tcctgaaagt
     3301 agagtagatt acatggatat atctcctaag caggttgtat ctgttgccac tgctatgata
     3361 cctttcttag aaaatgatga tgccaaccgt gcccttatgg gagcgaacat gcagcgtcag
     3421 gcagtgcctc tagttagaag agaagcacca attataggaa ctggtataga atacagagca
     3481 gcaaaagact ctggagcagt tgttgttgct agaaattctg gtatagcaga aagagtaaca
     3541 gcagatgaaa taattataaa aagagaagat ggaaacagag atagatataa tctacttaag
     3601 tttaaacgtt caaactcagg tacttgtata aatcaaacac ctataataaa taagggtgac
     3661 cagataataa aaggtgatgt tatagcagat ggtccagcaa ctgatttagg agaagttgca
     3721 cttggaagaa actgtcttat agcattcatg acttgggaag gttataacta cgaggatgct
     3781 atattaataa atgaaagatt agttaaagaa gatagattat caacaattca tatagaagaa
     3841 tatgaatgtg aagctagaga tacaaaacta gggccagagg aaataactag agatatacct
     3901 aatgttggag atagtgcaat caaaaactta gatgatagag gtataataag aataggtgca
     3961 gaagtagact caggtgatat attagttggt aaggtaactc caaaaggaga aactgaactt
     4021 actgctgagg aaagattact tcgtgcgata ttcggagaaa aagccaggga agttagagat
     4081 acttcactta aagtacctca tggtgaatct ggtataatag ttgatgtaaa agtatttact
     4141 agagaaaatg gagatgattt atctccagga gtaaatgaat tagtaagatg ttatatagct
     4201 aaaaagagaa agataaaagt tggagataag atggctggtc gtcatggtaa taaaggggtt
     4261 atctcgagag tattacctga agaagatatg ccattcatgg aaaatggaac gccattagat
     4321 ataatactta acccacaagg tataccatca cgtatgaaca tcggtcaggt tctagaagtt
     4381 catttaggac ttgctgctaa gacattagga tggtatgtag caacatcagt attcgatggt
     4441 gctaatgagt atgacataat ggatgctctt gaagaagctg gatatcctag agatggtaaa
     4501 ttaactttat atgatggtag aacaggtcaa tcttttgaca atagaataac tgttggatat
     4561 atgtattact tgaaactaca tcacttagta gatgaaaaac tacatgcaag aagtactgga
     4621 ccatactcat tagttactca acagccacta ggtggtaaag cacaatttgg tggacaaaga
     4681 tttggagaga tggaggtttg ggctctagaa gcatatggag cggctcacat acttcaagag
     4741 atacttactg ttaagtctga tgatgttgta ggtcgtgtta gaacttatga agctatagtt
     4801 aaaggtgaaa acatacctga gccaggaatc ccagaatcat ttaaggttct tataaaggaa
     4861 cttcaaagtt tatgcttaga tgtaaaagta ttgacagatg aagaccaaga aatagaagta
     4921 agagaatctg tagatgaaga tgatacgata ggtgaatttg agctagacgt tgtaaatcac
     4981 atgggagaag ttgaagaaag taatataata gaagaaattg aagatgattt tgtggaagcc
     5041 acagaagatg aggatataga gaatttagaa gaattcactg aagatgattt atttgaagaa
     5101 gaaatagatt ttgaaagtga tgactttgat atgtaaattt aattaatgta aatctcaaat
     5161 agaagggaga gaactccttg tttgaattaa acaatttcga gtcgataaaa atagcattgg
     5221 cttctccaga aaaaataaga caatggtcta gaggagaagt taaaaagcca gaaactataa
     5281 attaccgtac tttaaagcca gaaaaagatg gtcttttctg tgaaaggata tttggaccac
     5341 aaaaagactg ggagtgtcat tgtggtaaat atagaagagt tagatataaa ggtgtagttt
     5401 gtgatagatg tggagtagaa gtaactaaat caaaagtaag aagagagaga atgggacata
     5461 tagagctagc tgctcctatg tctcacatct ggtacttcaa aggtatacca agtagaatgg
     5521 gacttttact tgatatgtca ccaagatcgt tagaaaaaat actatatttc gcctcatatg
     5581 tagtagttga tccaggtgaa actggattga atgaaaaaca gttacttaca gaaaaagaat
     5641 acagaactgc tcttgaaaag tatggatata cttttactgt gggtatgggt gctgaagctg
     5701 taaaaacatt actacaaaat atagatttag aacaacaaag taaagactta agagcggaat
     5761 taaaagatag tacaggacaa aagaaagtaa gaacaattag aagattagaa gttgtagaag
     5821 catttaaaaa atctggaaat aaaccagaat ggatgatttt agatgcaata ccagtaatac
     5881 cgccagattt aagaccaatg gtacaacttg atggtggaag atttgcaact tcagacctaa
     5941 atgatttata cagaagagtt ataaatagaa ataatagact taaaagacta ttagagcttg
     6001 gagctccaga tataattgta agaaatgaaa aaagaatgct tcaagaagct gttgatgcat
     6061 tgatagataa tggtagaaga ggtagacctg taacaggacc tggaaataga ccacttaaat
     6121 ctttatcaga tatgttaaaa ggtaagcaag gtcgtttccg tcaaaactta cttggtaagc
     6181 gtgttgacta ctcaggacgt tctgttatag ttgttggacc agaacttaaa ttttatcaat
     6241 gtggacttcc aaagaagatg gcattagaat tattcaagcc atttgttatg gataaattag
     6301 ttaaagaagg ttatgcacat aatataaaaa gtgcgaaatc tatagtagaa aaagttaagc
     6361 cagaagtttg ggatgtttta gaagatgtta taaaaagtca tccagttctt cttaaccgtg
     6421 caccgactct acatagatta ggtatacaag catttgaacc aatcttagtt gaaggtaagg
     6481 ctataaaact acatcctctt gtatgtacag cttataatgc agactttgat ggtgaccaaa
     6541 tggcagtcca tgtaccttta tcagtagaag cacaagcaga agcaagattc ttaatgcttt
     6601 ctgtaaataa tatacttgct cctaaagatg gttcacctat aactactcca tctcaggata
     6661 tggttttagg ttgctattat ctaacaatag aagcccaaga tggagctaaa ggaacaggta
     6721 tggtatttaa agactttaat gaactattac ttgcttatta caataaatca gttcatctac
     6781 atgcattagt gaaactgaag gtaacacttg aagatggaag aagttcattg gttgaaagta
     6841 ctgttggtag atttatattt aatgaaaata tacctcaaga cttaggtttt gtagatagaa
     6901 aagaaaatcc atttgcactt gaagttgatt tcttagcaga taaaaaatct cttggtaaaa
     6961 taatagataa atgctttaga aaacacggaa atacagaaac agctgaatta ctagattata
     7021 taaaagctct aggatttaaa tattctacat taggtggtat aacagttgct gttgatgata
     7081 tgagtgtccc agaagaaaag aaagtattta tagctgaggc agaagctaaa gttgataagt
     7141 atgaaaaagc atacagaaga ggtctaatct ctgatgaaga aagatatgaa aaagttatag
     7201 agacatggac agaaacaact gataaagtta ctgatgctct tatgggtgga ctagatagat
     7261 taaataatat atatataatg gcacattcag gagccagagg ttctaaaaac caaattagac
     7321 agctggcagg tatgcgtggt cttatggcaa atgcatctgg taaaacagtc gagataccag
     7381 ttaaatctaa tttccgtgaa ggtttatcag tactagaata ctttacatct tcacatggtg
     7441 ccagaaaagg tcttgccgat acagctatac gtacagctga atctggatat ttaacaagaa
     7501 gacttgttga tgtaagtcaa gatgttattg taagggaaat agattgtgga acagaagata
     7561 ctacagagat ttatgctata aaagaaggaa atgaagttat agaagagata tatgatagaa
     7621 ttgtaggaag atatactata gaccctatat taaatcctga aactggagaa gttctagttg
     7681 aggctgattc aatgatacaa gaagatgaag cagaaactat agtagcttta gggattgaaa
     7741 aaattaggat aagaacagtt cttaactgta aaactaatca tggagtttgt tctaagtgtt
     7801 atggtagaaa cttggcaaca ggaaaagaag ttaatatagg tgaagcagtt ggtataatag
     7861 cagctcaatc tatcggtgag ccgggtactc agcttacaat gcgtacattc catactggag
     7921 gagttgcagg agctgatata actcaaggtc taccaagggt tgaagaatta tttgaagcta
     7981 gaaaaccaaa aggattagct gtaataactg aagtatctgg tagagttgaa attgatgaaa
     8041 caggaaagag aaaagaagta aatgtaatac cagaagaagg cgagactcaa acatatgtaa
     8101 ttccatatgg ttctagatta aaagttaagc aaggtcaaat gctagaggct ggagaccctc
     8161 taacacaagg atttataaat cctcatgata tagtaagagt aaatggtgtt aagggagttc
     8221 aagaatatat agttaaagaa gttcaaagag tgtatagact tcaaggggtt gatgttaatg
     8281 ataagcatat agaagttata gtaagacaaa tgctatctaa agtaaaagtt gaagacccag
     8341 gagatacaga tttattgcca ggtggatatg aagatgtatt aacattcaat gaatgtaata
     8401 aagatgctat agataaaggt ctaagaccag cagttgctaa aagagtttta cttggtataa
     8461 ctaaagcatc tcttgcaact gattcattct tatcagcagc ttctttccaa gaaacaacaa
     8521 gagtattaac agaggcagca ataaaaggta aagaagacca cttaatagga cttaaagaaa
     8581 atgttatatt aggtaagtta ataccagcag gaacaggaat gaagaaatat agaaatatag
     8641 ctgttgaaaa aattgaagat taatagcata tagagtaaag aaatcttgac aaccattatt
     8701 tgtggtgtta aaatagtaaa gtgtgttgtt taaaagtaca ctaagggctt gaacaagttc
     8761 ttagtgactt ttagatggca ataaatgaga actaaaaagc atcaagctag aaaagtgcta
     8821 atatcacata tttatgtgtt atagacatta aattgtaagt aacgaatgtt acaatcagtc
     8881 agtatgaata ttcatattga tgtagttggc ggtcactgat aagatcgaaa atttggaagg
     8941 aggtgcagat gatgccaaca attaaccaat tagttcgtaa aagtagaaaa gcgctagaga
     9001 agaaatctac tgctccagca ttacaaaaag gttacaactc tttaaataag aaagtaactg
     9061 acgctagtgc cccacaaaaa agaggggttt gtacttcagt aaaaacagtt actcctaaga
     9121 agcctaactc agctttaaga aaagttgcca gagttagatt aactaatggt atagaagttt
     9181 ctgcttatat accaggagaa ggacacaact tacaagagca tagtgttgtt cttataagag
     9241 gaggaagagt aaaagacctt ccaggggtta gataccatat actaagaggt actctagata
     9301 cagcaggtgt tgataagaga agacaatcaa gatctaaata cggtgcgaag agacctaaag
     9361 aagctaaaaa ataaaacaat taatgattga ttatagttcg gtgctttaaa tatatatata
     9421 tttacggcat cacggcatta aatgatttat gtcgagtacc tatgatttaa gttaaataat
     9481 taaggaggga agcgaaatgc caagaaaagg taatattcca aaaagagaag ttttaccaga
     9541 tcctatgtat gggagtaagg tagttactaa attaataaat aatctaatgg tagacggaaa
     9601 aaaaggaaag tctcaaagaa tagtttatga tgctttcgca attgttgctg aaaagacagg
     9661 agaagaagct ttagaagtat ttaataaagc aatggataat ataatgcctg ttttagaagt
     9721 aaaagcaaga agagttggtg gggcaaatta ccaagtgcca atcgaagtta gacctgaaag
     9781 aagacaaaca ttaggtttaa gatggctagt aaaatatact agagctcgtg gagaaaaagg
     9841 aatggttgaa aagctagcta aagagataat ggacgcagct aacaacactg gagcttcagt
     9901 taagaaaaaa gaagatactc ataaaatggc agaagctaat aaagcatttg ctcattacag
     9961 atggtaggat atatagatat cttataaatt caaggatatc ataaggagga taaccatggc
    10021 tagaaagttt cctttggaaa aaactaggaa tataggaatc atggctcata tagatgcagg
    10081 aaagactact actacagaaa gaatcctgtt ttatactggg caaactcata aaataggaga
    10141 aactcatgaa ggagcttctc aaatggactg gatggagcaa gaaaaggaaa gaggtataac
    10201 aataacttct gccgctacta ctgcttcatg gaaagaccat agaataaaca taatagatac
    10261 tcctggacac gtggacttca cagttgaggt tgaaagatct ctaagagttc ttgatggttc
    10321 agttgctgta ttctgtgcaa aaggtggggt tgagcctcaa tctgaaaatg tatggagaca
    10381 agctgaaact tatggagtac ctagaatagc ttttataaac aaaatggata tattaggtgc
    10441 agatttctac aatgttataa gcatgatgaa atctagatta aattctaatg cagttcctat
    10501 gcaattacca ataggtaaag aagattcatt tataggtata atagacttat taaaaatgga
    10561 tgctgttata tacaaagacg acctaggggt tgaaatggaa gaaacagata taccagaaga
    10621 tatgaaagaa ttagctgctg agtggagaga aaaattagtt gaatcagtag ctgaaactga
    10681 tgaagaatta atgatgaaat accttgaagg tgaagaactt actatagatg aattaaaagt
    10741 tgctataaga aaagctacta tagcttgtga aatgaaccca gttttctgtg gtacagctta
    10801 tagaaacaaa ggtgttcagt tagtaataga tgcagtttta gattacttac cagcaccaac
    10861 tgatatacca gctataaaag gtatattagc agatggagaa gaagctgaaa gacattcttc
    10921 tgatgaagag ccattctctg cattagcatt caaaataatg actgacccat tcgttggaaa
    10981 actagcgttc ttcagagttt attctggaac attagaatca ggttcttatg ttttaaatgc
    11041 aactaagaat aagagagaaa gaattggtcg tatattacaa atgcatgcta atacaagaga
    11101 agaaataact aaagtgtatg ctggagatat agcagcagca gtaggattaa aagatactac
    11161 tactggagac acattatgtg accctgctaa tccaataata cttgaatcta tggaattccc
    11221 tgagccagtt atatctgttg ctatagaacc aagttctaaa gcagctcaag aaaaaatggg
    11281 tatagctctt caaaaattag ctgaagaaga cccaactttc acagttaaga ctgaccaaga
    11341 aacaggacaa actataatat ctggtatggg tgaattacac ttagagataa tagtagatag
    11401 attattaaga gaatttaaag tagaagctaa agttggtgca ccacaagttg catatagaga
    11461 gactataact caaccagttg atgttgaata caagtactct aagcaatcag gtggtagagg
    11521 acaatacggt cacgttaaga ttagagttgc tcctcaagag ccaggtgaag gatacaagtt
    11581 cacaaacaaa actgttggtg gatcagtacc aaaagaatat gttggtccag ttgacatggg
    11641 tatacaagga gctatgcaat ctggtatagt tgctggatat ccagttgtag acgttgctgt
    11701 tgagttatat gatggttctt accatgaggt tgactcttct gagatggcgt ttaagatggc
    11761 tggttcaatg gcattcaaag atgctatgaa gaaaggtaat gcagttcttt tagagcctta
    11821 cttcaaagta gaagttgtta ctccagaaga ttacatggga gatgttatgg gtgacttaaa
    11881 ctcaagaaga ggattaatcc aaggtatgga agctagaagt ggtgcacaag ttataaatgc
    11941 attcgttcca ctttcagaaa tgtttggata ctcaacagac ctaagatctt caactcaagg
    12001 tcgtgcaact tacacaatga tattcgatca ctatgagcaa gttccagctt cagttgctaa
    12061 gaaaatagct gaaggaaaat aatttaaaaa taattaataa tataaatata aaaaataatc
    12121 agctagatga gatatatctc atctagcaca agat
//
LOCUS       DAMJVX010000107        11954 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMJVX010000107 DAMJVX010000000
VERSION     DAMJVX010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11954
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.111"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            152..865
                     /locus_tag="PC407_002528"
     CDS             152..865
                     /locus_tag="PC407_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HDF2497965.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLFLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            983..1852
                     /locus_tag="PC407_002529"
     CDS             983..1852
                     /locus_tag="PC407_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2497966.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSITSNIITV
                     IPEKSRVEVLQVDGDWSKIVYDDKIGYVFNYFLSTDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(2007..3152)
                     /locus_tag="PC407_002530"
     CDS             complement(2007..3152)
                     /locus_tag="PC407_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2497967.1"
                     /translation="MSTYYVPPINLLGKGCLNEAKDSIKSLGTKKAFVVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVDKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIVAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAVEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKEKIIELFMKIS"
     gene            complement(3485..4261)
                     /locus_tag="PC407_002531"
     CDS             complement(3485..4261)
                     /locus_tag="PC407_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2497968.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            4843..5817
                     /locus_tag="PC407_002532"
     CDS             4843..5817
                     /locus_tag="PC407_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2497969.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            5915..6220
                     /locus_tag="PC407_002533"
     CDS             5915..6220
                     /locus_tag="PC407_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2497970.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGNEISFKLKDKTVKTITALDNI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            6376..7536
                     /locus_tag="PC407_002534"
     CDS             6376..7536
                     /locus_tag="PC407_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2497971.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            7586..8488
                     /locus_tag="PC407_002535"
     CDS             7586..8488
                     /locus_tag="PC407_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2497972.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESIFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            8568..9461
                     /locus_tag="PC407_002536"
     CDS             8568..9461
                     /locus_tag="PC407_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDF2497973.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNKLNIC"
     regulatory      9514..9695
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            9923..11053
                     /locus_tag="PC407_002537"
     CDS             9923..11053
                     /locus_tag="PC407_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2497974.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            11125..>11954
                     /locus_tag="PC407_002538"
     CDS             11125..>11954
                     /locus_tag="PC407_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2497975.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     "
BASE COUNT         4504 a         1372 c         2128 g         3950 t
ORIGIN      
        1 attattaagt gaggttaagc gttataatta attataaata ttgaataatc taaatggata
       61 agataatggt attaaaaatt atcttaaaag gtgaaataat ctgtatttaa tgtagtacag
      121 ttatatcttt aaagcaaggt ggtgaaatga tataaataag atatctaaaa gagggtttga
      181 tgatagtgat aaaagatggt tttcaagtaa agaattaaca agacttataa aggctaaaga
      241 agaaatagaa tggttattaa atagggatta caaaatagac ccagtcgtta cttttgttgg
      301 tgatagatat cagttttcta taagacaaag agatgcatta aaaagagcag tctgtactga
      361 tgaaaaaaat attataagac aatcaaaaag gctttttcta gataaaatca aagaagatac
      421 cgtaaatata gatggcttta atttgataat atccatagag gtagcattat ctggtggtac
      481 tctagtaata ggaagtgatg gaaatataag agatttagca ggtcttaggg gaacctataa
      541 gattatagat aaaacagaag aagctttgaa tctgataggt aaatttttta ataaatacaa
      601 tgcacagaat cttaaatttt atttagatgc tccagtttct aattcaggaa atttaaagta
      661 taggatatta gagcatgcca aaacttgggg aattgaaact gaagtagaat tagtaaaaaa
      721 tgcagatgtt gtattggaaa aattagatag agtagttagt agtgatgcag tcatagttga
      781 caagtgtata agttatttta atgtggctag aggtataatt gaagagtata ttaaagaatg
      841 taatatcata aatttaaata aatagataaa tagaggtgag aatatataag tatatgtagc
      901 aatattatta gtaatacaag taaagtttga aaataggaga acgaatacat atcgaatttc
      961 ataaaatgtt gtaaaaagac atatgagagg atggatatgt gtgaaattaa ctaaactaaa
     1021 tattaaaaaa tatagagcac ctatatataa atatgcttta gcaaatgtaa atttgaggtc
     1081 tgctaaatct actaattcaa gtatcataac agtaatacct cagggagcta aaatggaagt
     1141 attggatgaa gaagatgact ggattaaggt gatgtacaat tctcaagaag gatatgtgta
     1201 taaggatttg gtatcagtaa gcgaatatgc ttggagtaac ttaaacttaa gagaagataa
     1261 atctataact tccaatataa ttacagtaat tcctgaaaaa tctagggttg aagtccttca
     1321 agtagatgga gattggagta agattgtata tgatgataaa ataggatatg tttttaatta
     1381 ttttttatca actgatggga ataaaccaaa tgaattagat tataagtatt tttatactga
     1441 catgattaaa tttgttaacg aaaataatat taaaagtact agcgattatc taattgtaac
     1501 agaccttaga aataaatata cttatatttt taagaaagat aatggaggct gggggcaact
     1561 ttacaagtgg caatgtacta taggcaagcc tgagactcca actataacag gtatatttta
     1621 tataagtgga agaaaacctt cttttggaac ggatgagtat tcagttaagt atgctactag
     1681 aattaaaggt ggatattact atcattctgt gctatatgat tcaacaggaa gttatataat
     1741 agatggcaga cttggtgaag ctcttagtca tggatgtata agattaagta cggaaaatgc
     1801 aaagtggata tatgataata taccagatac aacaacagtt ataattcatt aagaaaaatt
     1861 ttgagttttt attgggataa tgattctcaa attaatagga ttatatatta acttcttcat
     1921 aaagagtact atttaaaaag tgtaataaga gagtgctatt aacaaaacaa agatattttg
     1981 aggcactctc ttataaatat acataattaa gatattttca taaacaattc tataatcttc
     2041 tccttatcaa agaaaacagg attagcacca gcacaagcat ctttcataga gttttctgca
     2101 agcaattcaa aattaggatt atcaacacct acatctttta aagattttgg gattccaacc
     2161 tcttcagaaa gagcttttat cttatctatt acaaagtcaa cacactcttt atctgattta
     2221 ttatttacat ccattccaat aacttcagct attggtcgga atttggcagg ggctgattta
     2281 gcattttcct cctcaactat tggaagtagc atggcattac atactccatg aggtaaatca
     2341 tataaacctc ctaattgatg agccatagca tgtacatttc caagacctgc attactaaag
     2401 gcaattccat ttaaaaaaca agcataacac atttgttctc tagcttctat gtcattacca
     2461 tttttaacag ctcttggaag gtatttaaat atctgcttta tagcatataa ggcagttgaa
     2521 tctgtaacat cataagcacc attagctact acagcttcta cagcatgagt tagtgcatcc
     2581 ataccagttg ctgctgttaa attagatggt ttgctaatca ttaattctgg gtcatttact
     2641 gtcattgtag ctagtgagtt ggtatcaacc attatcatct ttacatgtct gtcttcatca
     2701 gttataacat agtttattgt aacttcagca gatgtaccag cagtagtagt tatagctacg
     2761 ataggaagac attttttaga agttttgttg ataccttcat agtctctaat atctcctcca
     2821 ttagtagcta atatagagat agctttacca cagtcttgtg gagaacctcc tcctatagtt
     2881 ataacgaagt cacaattttc tgattttaat attttaagtc ctttatccac attagttacg
     2941 gttggatttt gtttaacttc atcataaaca acaaagtcta catctatctg agataaaata
     3001 tctgttactt tttttacagt accatttgaa actagaaatt tatcactaac aacaaatgct
     3061 tttttagttc ctaatgattt aattgaatct ttagcttcat taagacatcc tttcccaagt
     3121 aaatttattg gtggtacata ataagtgctc ataactaaaa tctcctttta ttttcattga
     3181 tttttatgta aaaatatata tctaacaatt ttcactaatt tactcataat atatcgtatc
     3241 ttagattgag atagggttac tggttcttat ataagaactt aattcgtaat tacgattatg
     3301 attagaattt aacatagtat ttttatcatg tcaataaaaa tataaaaaat attataaaaa
     3361 aaacaaagtt actgtagtta aatcagcaac tttgttttta tagtataaat caaataaata
     3421 cattagttta aaacaaaatt tttaattatt atgttttgat atcttccata tacagaatat
     3481 taatctaatt tattacaatg tcttcattac gttcttccat cattgattga atatttttct
     3541 gagcatttaa gagtaatttt tttatcagtt ctattttttc gtcattagct ctaaataaag
     3601 gcagtgttac actaacagca gctcttatac atccattttt tcttattggt acggcaagac
     3661 attttaggtg ttccattgat tcttcacact ctgaagctat ttgtgttctt tgaacttcaa
     3721 gaagctggtc gtataaaaca tcaaaatctg taattgattt ttctgtaaaa ccttttagac
     3781 catctggata taagcttatt agtttttcct tgtcaaaatc acataaaaga gcttttccaa
     3841 gacctgtaca attagcaggt attttttttc caacataaga aataagtcta attggttctg
     3901 gagaatctac ttttccaata taaagaactt cattattgtc aagtatacca agctgacaag
     3961 tttcagaaca ggaattaact atattagtca tttcgtcaat tatatagtta aaaatagttc
     4021 ttttttcaaa aaatgaagac cctaatgtgt aagaacgtat ccctatagaa tatttggatg
     4081 tgtttttatc aatagtaatg tattttctag cattaagtgt atgaactata ggaaagatgc
     4141 tactttttgg agcattgata acatctgcta tttctgtaag agtatatcca ttttcggatt
     4201 ctgctaatgc ttctaaaata tctaatatcc tagctgttgg gcgatgatta tctgaagtca
     4261 taaatttccc cctcttttta ttaatttaaa gtattagaat atgttgattt tagcacacta
     4321 tacgatattt tacaatgtag aaaataaata tgtaaattta tatataaata agctcttgat
     4381 ttagatacag atagatattt ttttagactg tacattaagg aataactaat ttccaaaata
     4441 tacctaaaga aagtaagtga tatttagtta tataatttaa gtaaatatat gcaatagtta
     4501 tttttatatg taaatatcaa aatattatga atttatattt tatctatttt ttacactgta
     4561 taaaaacttt atagttttga atactatata attttaaata cgctgtattt ttgcatataa
     4621 ggaagtcatt tgataatata caaggtatta tcagaaagtt tgaaaaaaat ttgacaaaaa
     4681 tttatttttt ttattgactc ataaatagga aaatgctatc attaattcaa agattcgtaa
     4741 ttaaaaactt agttcttaaa tacgaaccga aacggccgtt agaattaaag ctaaaaactt
     4801 aataagagta attaaatatc atgttttagg aggattaact ttatgataat ggattttatt
     4861 aaaaaaattc ctgcaggtat gatgatagtg ccaatgttta tagcagcttt cattaatacc
     4921 ttttgcccag atattgtaca aataggttcg tttacgacag cagtattttc aagtgttggg
     4981 tctgcaacta taattggggt tcaactggta tgcttgggaa ctcagcttca atttagagaa
     5041 atgccaaaag ttttaaagag aggtggtata cttcttggtt ctaaatttgc tataggtgct
     5101 ttgataggta tacttatagg aaagctattt ggaatggatg gtgtccttgg tcttacaaca
     5161 ttggctgtta taagtgcagt tacaaatagt aatggtagta tatatctttc attgatgact
     5221 tcatatggag atgaaacaga ttgtgcaact atgagtttac ttactttaaa tgatggacca
     5281 ttttttacat taatagctct tgggacttca ggtcttgcaa atgtgccatt gttgtcatta
     5341 ttagctgcaa tagtgccaat acttgttggt atgataatag gaaattttga caaaaaaatg
     5401 aaagagtttt tagagccagg gtgtaattta ctcgtacctt ttgtagggtt tgcacttggt
     5461 agtagcataa atcttgcaag tatactaaaa ggaggtttat caggagtatt acttggatta
     5521 atatctgtat ttgtaggagg tatatttata gtattctgtg ataaatttat aggaaaacgt
     5581 ccaggatatg caggatgggc tgtagctaca accgctggaa atgcaattgc tgtacctgct
     5641 gctgttgcat tagttgaccc atcatggcaa ccatatgttg caacagctac aacacaagtg
     5701 gcagccgcag tagttgtcac tgcaatatta gtacctctta taactagttg gtgggctaag
     5761 aagtatggat gtcctaagtt cccaaaacaa aatgaggatg gtacaataac agcttaattt
     5821 atattacagt gagttgttgg taaaataata aattctttaa ttagatttta ggatattaat
     5881 aaaattatat tatttataaa ggagagcaag aataatgata aatgcagtga taattgatga
     5941 aaaagataat gttgcagtag ctattgaagc aatagctaaa ggtaatgaaa ttagttttaa
     6001 attaaaagac aaaacagtaa aaacgattac agcactagat aatataacta tttatcataa
     6061 attggctata aaagatatgg cccaaggtga taaagttact aaatatggtg aacatatagg
     6121 agaagcaaat caggaaataa aaagtggtca acatgtacat attcataatg ttagaagtgt
     6181 tagagaggat ttggataaac aagttatgac taatatataa aataatattt atagagttat
     6241 aaaagttgta ttcattgctt ttagtgtcaa atagttttta aataggattt tagctgtttg
     6301 attagtgagt gtaataaaaa ttataatata taaaaattat aaaaagtata tagactattt
     6361 taggaggaaa tcagtatgaa attttatgga tataaaagac cagatggaag agttggtata
     6421 agaaaccaca tattaatatt gcctacgagt gtgtgtgcat cagatacaac tagaataata
     6481 gcatcacaag ttcaaggggc agttaccttt aataatcaga atggatgttc acaagtacat
     6541 tcagaccaac aattaactat ggatgttatg gctggttatg cagcaaatcc aaatgtatat
     6601 ggtgtaatag ttgtatcttt gggatgcgaa aattgccaaa atgatttggt agttgatgca
     6661 ataaaagaga gaacaaataa gccaataaaa actcttgtaa tacaagaaga acatggaact
     6721 ttaaaaacta tagaaaaagc tgttcgttat gctagagaaa tggcacaaga agcttctttg
     6781 cttagaaaag aagaatttcc aatatctgaa cttatactag gaactgaatg tggtgggtct
     6841 gacccaacaa gtggattagc agcaaatcca ttaataggtg aattaagtga taaacttgtc
     6901 gatttaggag caacatcaat tttaagtgaa actactgaat ttataggtgc agagcatata
     6961 ctagctagga gagcagtaaa tgaagaagta aaagaaaaaa tattacatat agtacataga
     7021 tatgaaaatt cattaaaatt ggttggagaa gaagttagag aaggtaatcc atctcctgga
     7081 aatatagctg gtggtttaac aagtttagaa gaaaaatcac ttggttgtat acataaagga
     7141 ggtcatagac aaataagtga agtatatgat tatgccaaac aaattgataa gaaaggtctt
     7201 gttataatgg atacccctgg aaatgatgct tcatctgttg ctggtatggt agcaggagga
     7261 gcacaaatag tggtatttag tacaggaaga ggaacaccat caggaaatcc aatagcacca
     7321 gtaataaaaa taactggaaa taaaataact tttgcaaata tggaagataa tatggatttt
     7381 gatgctagtc cagttatcta tggtccacaa acaatggaag aattaacaga tgatttgtta
     7441 aatatggttg tagatgtagc aaatggaaaa caatcaaaag cagagtccct tggatatact
     7501 gaaatggcaa tagctagagt atgcaactat gtataatctt taagtaagaa aataaataca
     7561 tagaatttta ataaagggtg agattatgaa gtttgtaagt tttactgaag gagaaaatga
     7621 atgtaaaaga actggagttt tttctaaaga tgaattattt attatagatg ttaactcttt
     7681 aaatctcagt agaaatttta aggatttaaa tgaattaatt caaaatgtat ctagtgaaga
     7741 tattattaag ttgaaaacta ttataaatga aagtatattc tataattatg atgtttttaa
     7801 aagagagaga tgtactgtac atgttcctat agaaaaacca atacatgata tcttatgtgt
     7861 gggagtgaac tataaagacc atcttgaaga aactcaaact cactttgatg atagttttga
     7921 agagccacaa aaaacggttt attttacaaa acgtgtgaca aaggcaatag gtccagaaga
     7981 tgagatagat ggtcattttg agataaataa tcaactagac tatgaagttg aacttggagt
     8041 agtaataggt aaaacaggtg ttaatattag aaaagaagag gtagaagatt atatctttgg
     8101 ttacactgtt ataaatgata tttctgctag aaagttgcaa aaagaacatg ttcaatggtt
     8161 tagaggaaaa ggattagata catttacctc tttaggtcca tgtattttac ataaaagtgc
     8221 agttcctttt cccataaaac ttaaagtatg tagtagggta aatggtgaag aaagacaaag
     8281 ttctaataca ggattattca tatcagatgt ttcagacata gtttcagaac tttcaaaagg
     8341 tatgacttta gaggctggag acattatagc tactggaaca ccttctggag tagggatggg
     8401 attttcacct cctcgatata tgagtagtgg agatgtagtt gaatgtgaga ttgagagtat
     8461 tggaatttta aaaaattaca ttaagtaaat gttttaatat aattaatatt aaagctaaat
     8521 ctgatgtatt aatgaacata ttaaaataaa ttttaggagt ttaaattatg aaagcagaat
     8581 ttttgacacc agttgttaca atatttgatg aagaaggaaa tttagataaa gaaggtaata
     8641 aaaaagtgta cgaccatctt atagatggag gtgtagatgg actagtcata atgggaagta
     8701 ctggagagtt ctttaatatg tctatggata tgcaaaaaga acttatagat ttagtaacta
     8761 gtcatgtaaa taagagagta aaagtgttta taggtacaag tcgtatgtgt ataaatgaat
     8821 ctatagaact ttcaaactat gcacataata gtggagcaga tggcgttatg ataataagcc
     8881 cttattattt ctctcttgat gatgatagta ttgagttatt ttatagtgaa atagctaaga
     8941 atacagaggc tgaaatatat ttatacaatt atccagatag aactggttat gatttaagtc
     9001 cagaacttac attaagactt gcaagaaaac ataaaaacat agttggatat aaggatacag
     9061 taactttgat gggacatacc agagatttaa taaatactat gaaaatagag ttcccagaat
     9121 ttaaggttta tagtggttat gatgaaaact ttatacataa tctaatgtct gggggagcag
     9181 gttgtatagg gggattatca aatttaattc cagaagtttg ctccaaatgg gctgaagctt
     9241 acaataatag agatatggag aaggttatag aaattcaaaa atatattaat aaagcaatgg
     9301 atttttactt aataaccaat ccatttatac cagcaatgaa aaaagctatg aatatcagag
     9361 ggttacaaat aagtgattat atgactgctc catttgtaaa accaaatgaa aaacaagtat
     9421 gtgaaattaa aaaattgatg aataaattaa atatatgtta aatcaacctt gcaaagaggt
     9481 caacaataaa ttacaattat cttaataaaa attaaattaa aataatacta gggtactata
     9541 ttagtttaat agggaaagtg atgaaaattc actacagccc ccgctactgt atacggatac
     9601 gaaatcaaaa tccactgaaa tttataaaaa ttttgggaag ggtgatggag gaaaagccgt
     9661 gagtcaggag acctgcccag tattatataa gttcaattta gggtggagat tgtaatttat
     9721 aataaaatga agtatcaatt cgtttttgta aagttgatat atgaacttct ccttattaaa
     9781 ttaggagaag ttttttattt tattaaaagg ttatacttat taaagatgac tttaggataa
     9841 ctttaataag tatactttaa aaagcatata ttattaaagt tttttagcca ctcataaaaa
     9901 ataagatatt ggagtgaaag agatgaaatt taaatcaaat gttttaaagc agtttttttt
     9961 aattgctatg gtaattttta tggtaggagg tcttataggg tgtagtaaaa gtgacaaaga
    10021 gaataaacaa gctagtactg gtaagaaatt agaattaaaa tatgctagtg gatttagtgt
    10081 tgaagattta gaagatggaa ttaaaaaagt aactgatgga gataatcgag aactgatttt
    10141 aatcccaaag aagattaaaa ttcctgaaaa atataaaaat gctaatatag ttagaacgcc
    10201 tgtggacaat gtattaatag catctacaac acaggcttgt tctctgagaa caatagatga
    10261 actagatagt ataaaagccg taacaacaga agagcagtac tggactatta aagaaattaa
    10321 agatttaatg aaagatggaa aaatacatta cgttggttca aatttagcac cagattatga
    10381 aaaaatagta gacttaaatc ctgacttaac tatagtgagt agtggtttat cagaggctga
    10441 tacaaaattt attgacaaat taaaagaatt agggcttaat tatgttgtag acaatgagta
    10501 taaagagcaa aatccatttg gaagaatgga gtggacaagt ttaatagctg cattttatga
    10561 taaagaagat atggctacta gtgaactaaa taaaactgta caaaaggtag aagaagtaag
    10621 taaaaaaata aaaaacaaag ctaaacctaa agttgtatgg gcatctgtat atgaaggaag
    10681 tgcttatata gctcctaaag attcatatgt ggataatatg ataaaaatgg ctggtggaat
    10741 aaatgcatgt gcttcaatag attcaaatga agggcgagta agtattgaac aattgcatga
    10801 agttgcaaaa gatgcagata tatttgtata ctcatcaagt tcagattatg caccaaattt
    10861 agattctata aaatctagtg cacctgtatt ggctgatttg gatgttgtaa aaaacggaaa
    10921 cctatgggtg tttgctccag attattatca atcagtagat aagacggacg aattaatagt
    10981 agatttagtt gagatgttcc acccaggtac tacaggtgaa aaaatcaaac attatataaa
    11041 ttatgataaa tagtacagag aggacacaaa aagataggtt ttatagattt tagttaatat
    11101 aactttgcta agaggagtat acaaatggat tttatcaata aaatgagaat aaggaatacg
    11161 acaatattta tagtattaat tataagtatg atacttactt ttttagtgtg tgtagcattt
    11221 ggctctgtaa aaattgaatt taaagaagta atagatgttg tattaagagg tatggataat
    11281 acagaacatt ataaaataat atacaatata agaatcccta gagtacttgc aaccatggta
    11341 ggtggagcat gcctagcagt atcaggtatt ttattacaga tattttttaa aaacccaata
    11401 gttgaaccgt atatattagg agtatcttca ggttctactt tatcagtagc tctcatgatt
    11461 ttgggaggat acacattggg atttgagagt gtaaattcaa tgagtatatt tgtagcagct
    11521 tttataggag ctttaattgt aatgtctata gtcattttat ttgcaaataa ggtaaagaat
    11581 ataacaactc ttttaataat aggaattatg accggatatg tatgtagtgg tataacaaat
    11641 gtgttacaat catttgcaaa tagtgataaa ttaaaagaat ttactatgtg gtctatggga
    11701 agtttttctg gatttacatg ggaaaaggta gaaatactta taatagttgg tatattaggt
    11761 cttttgatga ctgtattcat aataaaacct ttaaatgctt ttttattagg agaagagtat
    11821 gcaaaaagta ttggcataaa tataagattt tttagagttt tgatagtgtt tatatctagt
    11881 atattagcag caatagtatc agcatttgca ggacctatag ggtttattgg acttgcagtg
    11941 ccacaaattt caaa
//
LOCUS       DAMJVX010000108        11847 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.112, whole genome shotgun sequence.
ACCESSION   DAMJVX010000108 DAMJVX010000000
VERSION     DAMJVX010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11847
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.112"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            151..2244
                     /locus_tag="PC407_002539"
     CDS             151..2244
                     /locus_tag="PC407_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2497976.1"
                     /translation="MNKRNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEQNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTILNKPIKINEFNEISNTKEGKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLIYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTQSHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
     repeat_region   2905..3198
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2905..2933
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaat"
     gene            complement(3366..3650)
                     /locus_tag="PC407_002540"
     CDS             complement(3366..3650)
                     /locus_tag="PC407_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497977.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     gene            3857..4675
                     /locus_tag="PC407_002541"
     CDS             3857..4675
                     /locus_tag="PC407_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2497978.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            complement(4808..6928)
                     /gene="feoB"
                     /locus_tag="PC407_002542"
     CDS             complement(4808..6928)
                     /gene="feoB"
                     /locus_tag="PC407_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2497979.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(6954..7181)
                     /locus_tag="PC407_002543"
     CDS             complement(6954..7181)
                     /locus_tag="PC407_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2497980.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            complement(7508..7948)
                     /locus_tag="PC407_002544"
     CDS             complement(7508..7948)
                     /locus_tag="PC407_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2497981.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     INLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            8100..9089
                     /locus_tag="PC407_002545"
     CDS             8100..9089
                     /locus_tag="PC407_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HDF2497982.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     misc_binding    9241..9493
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9565..10773
                     /locus_tag="PC407_002546"
     CDS             9565..10773
                     /locus_tag="PC407_002546"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDF2497983.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     gene            complement(10834..11715)
                     /locus_tag="PC407_002547"
     CDS             complement(10834..11715)
                     /locus_tag="PC407_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2497984.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPGRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
BASE COUNT         4486 a         1434 c         1739 g         4188 t
ORIGIN      
        1 tatcaattat aatttaaaat atgaaataat aaactttcta attaaattaa ggtatttagt
       61 gtgtatgtat aagttataag taaatcttct aaaaagaata attttaacag tgttgttata
      121 aagagcagta actatggagg agataagtat atgaacaaac gtaattttga agttatatta
      181 aatcaactac agattaatat atatgttaca aatattcata ctaatgaaat tatttttatg
      241 aacaagaaaa tgaaagaaga atataatatt ttagatccag aaggaaaagt ttgctggcaa
      301 gtattatatc ctgagcaaaa ttctacttgt agtttttgta aagttttaga actattaaag
      361 aatgataaaa aaggagtatt aataaagtgg tatgaaaaat gtaataagtt aaatcgtgtt
      421 tttgaaaatt atgatagttt aattacatgg caagatggca cagttgttca tatgcatcaa
      481 tcaatagata ttgcaaactc aacaatctta aataaaccaa ttaagattaa tgaatttaat
      541 gaaatatcaa atactaaaga aggaaaagga gtttttaatt tttcaagaga caattttgat
      601 tataattcta cattgcttta tgatgctttg attagaggta cagatgaata tatatacatt
      661 tgtaatatga aaacaggcgt atttcgatat tcaccatcac aagttgaatt atttgactta
      721 ccaggagaaa ttgttgaaaa tccgcttatt tactggaaaa aaattgtaca tccagaagac
      781 tggaatcgtt tttataagtc aaacactgaa ataggtaaaa accagatgga ttatcataca
      841 gtggaattta gggctaaaaa tcgtagtgga gaatatatat ggttaagatg tagaggacaa
      901 ctaatgcgtg atgaatttgg agaaccaagt atttttgcag gaataatgac gcaattaggt
      961 aaacaaaata agattgattc attgacacag ttactgaatt atcatgaatt tatgagtgta
     1021 tttgaagata aaatttcaaa tcctatgatt gaaaaattat gcatagtatt attaaatatt
     1081 gacgatttta aaaatgttaa tgagatgtat gatagggatt ttggagataa tataataaaa
     1141 acactagcac agtctataca atctatttta ccagataatg ctgaacttta taaacttgat
     1201 ggggacgaaa tgggtattct tgttgataat gttgaagaaa atgaaattct aactctatat
     1261 aatcaaatac agaatatgat tatacacctt caactatgga gaaaatatgg attaaatata
     1321 acaatttctg ctggttgtgt aatatatcca aaacatggag atacagtaaa agaactttat
     1381 aaatgcgcta gttactcttt acaatatgcg aaagagcatg gtaaaaacag gcttgttttt
     1441 ttctcccaag aaatattaaa aaataaaatg tattcattag aaatgatgcg ggatttaaag
     1501 gcatcaatta atgatgattt taggggattt agtcttcgat tccagccaca agtagataca
     1561 caaagtcata aaataatagg tgtagaagtt cttttaaggt ggactaatga caaatgcaaa
     1621 gcaatttctc ctttggaatt tattccaata ttagaagaaa atgatatgat aaatattgta
     1681 ggggcttggg tgcttcgaat ggcattacgt acttttagga aatggattga ttactatcct
     1741 ttttttaaag tgagtgtaaa cgtttcagct gttcaaatat tagaagacac atttattgaa
     1801 gatatagtaa aaattataga tgatgaaaat tttccttatc aaaatcttgt tttagaattg
     1861 acagaaagtc atacagtaca aaatatgagt attttacagt ttaaatttaa ggctttacaa
     1921 gatttaggaa tttatattgc tatggatgat tttggtactg gatactcgtc tctagaagtt
     1981 ttaaaatttt ctccaataga tatagtgaaa attgatagag tatttgttaa agatatatta
     2041 aagagtaagt ttgatgctac atttatacat tttattgttg caatttgtca tgatgtagga
     2101 ataaaagtat gtttagaagg tgtagaaaca caagaagagt atgatttagt taaacaaata
     2161 aagccagatt acatacaagg atatttattt ggaaaaccgc agacagcaac agaaatcttt
     2221 gaccttttaa agctagataa ttaattctaa tttatcaata atatcactga aaagctttaa
     2281 aatcaaaaat acattaaatt attatattga agatatatca attataaatt gcaatataag
     2341 aaaatagcat ttcaagggat gttaaattag gagtagtata tgattaaagt attttaaaaa
     2401 gggataacta taataaaaat agatatctat ttttagatta aaaataatat atcataaata
     2461 aaataataag aggtagatac agttttaagg gaatacaaaa gtttttaatt aaactatgct
     2521 tgttcagata gatatttatt taagaaaaaa gactattaaa agcaatatac aagaatgata
     2581 tattagattg attaaacaag cataaatatt atgtaaaaaa ctttaagtta tagaatttaa
     2641 atccaatgta gatagattac gttttttctt gcttttatta tggtataaat tggtatcaat
     2701 attcaaaagt aatatattta tgatataata aaattataga aattttgcag tgagctatat
     2761 ttgtgataaa atttggcgta acagttgaaa tataaggtgt tgagagtgca tgataagcgt
     2821 tatcaattgc actattgctc gttcactgca aatttaggag agttgtatac gtgtaagtgt
     2881 tgaaaatact aagtttattt tggggtttta tattaactat atggaatgta aatctcggag
     2941 aatttcctaa tgtggattta ataggaagtg ttttatatta actatatgga atgtaaattt
     3001 tagcaaattt ttcgtaaaag tcactatttt caatggtttt atattaacta tatggaatgt
     3061 aaatacatat aaaactttca tcttctgtta attcattaaa acgttttata ttaactatat
     3121 ggaatgtaaa tccatctttt gttgttttac ataaatttat attacttacg ttttatacta
     3181 actatacgga atataaattt ttataatttt cttttaatgt gttatcttat gattatctag
     3241 ttatattagc atacaaaaat ataataaaat tacactatat tataaaaacc aaaaggtagt
     3301 atagaaatcc tattaccttt ttattattaa ttttatcagt gttttattat aaatagctta
     3361 cataattaca cattttttct gattaaataa tatgatgcta ttgttataat agatactaat
     3421 gctaatgatg ttattgcagt atctaatctt cttattaata aactcatttc tatatattca
     3481 agttctatta aatattgata aaatatttct agtttaacag ctttttctct atctatattt
     3541 ccatacttta actctaattt atctaaagat tttttattac aataattaac tttatgttta
     3601 attaaatatc ttattgtagc acctattaca attttcactc taacaagcat aaatataata
     3661 ttccatccaa aagttaagag gggatatctt ttttatgaga ggaatggata aaatatttat
     3721 aactgacatc taactgatat ttaagcttta aaatcaaaac catattgatt ttctaaaata
     3781 ataactactt gatttagtaa ataataaaga atatagtaac tatatagaca acaaaaagaa
     3841 aaggagtgta taacccttgg aaaaacagta ttttacaaca ggtgaatttg ctaaattgtg
     3901 tggtatatct aagcagacat taattttcta tgacaaaata ggtatttttt ctcctgaata
     3961 taaagataaa aataattata gatattactc agtttatcaa tatgatacac ttgatatatt
     4021 acaatcatta agagaaatag gaatgagttt agaggaaata aaagagtata tacaaaatag
     4081 aactccccag ctttgtgtga aaatgcttaa agaagaagaa aaaaagataa gagaaaaaat
     4141 aaaaaaacta agaaagataa gctctaagat gcaaaataga ataaatataa cagttgaagg
     4201 aatttctaaa atgaatagtg aagaaattta tatatccaaa aagtttgaag aatatttagt
     4261 tctatcaagc gatttgagta atatgagtaa tagtgatttt atgatggaat taatagattt
     4321 caccaattat tgtaaatcga aaaatttgta tcaaggatat gagttgggag taatagtaag
     4381 taatgaaaat ataaaaaatg gggactattt aagtatatca agtttttatt tgaagataga
     4441 taaaaaaata aaggataaaa agctatatgt aaagccagaa ggaatgtatg cttgcataaa
     4501 acatattgga aaatatgagg attcatataa atcttacgaa aaattgaaaa aatatatata
     4561 tgaaaatgaa tataaaatta ttggaaattc gtatgaggag agtatacttg actttttttg
     4621 tgaaagtaat gaagataatt atatgactga aatttcaatt caagtatcca gataagaatt
     4681 aaaatagaga tatattcaaa tgttaagtaa ttttacttat cattgaatat atctctatgc
     4741 tttataataa agtatataaa tttttaaata gtttctctaa tatatgtcta atgtcagtta
     4801 atactaatta tataaataaa ttgtaaactg tagttgttaa aaaacaaaca acaaaagcaa
     4861 gtatagtagg cattaagaat cctaaggcag tccattttaa actaccagtt tcttttttta
     4921 ttgttaaaag tgttgttgca caaggccaat gcagtaaact gaacagcata acatttaaag
     4981 ctgtcaaata agtccatcca tgttctctaa gaacttgacc taaagcacta aagctatcta
     5041 attctatcat agaaccagtt gctaggtaag ccattaaaag tataggtaca actatctcat
     5101 ttgctgggaa tccaagaata aaagcaagta atataaaacc atcaagacca attaatttag
     5161 ctaaagggtc taaaaagttt gcaacatgtg ataatatgct taaatctcct atatatatat
     5221 ttgcaaatat ccaagttatg acaccagcag gtatggctac cattacagcc cttcctaaaa
     5281 caaatattgt tctatctatt atagaggtat ataaagttct tcctatttga ggaactctat
     5341 aaggaggaag ttctaatgta aatgtagacg gaactccttt taatagcgtc ttagaaagag
     5401 tataagaaac caacaatgtt attataacac ctagaattat cagtaaagtt atgcaaagtg
     5461 cagttgctac gcttgatacg aaagagttgg ttataactga gctaaaaaat attgtagata
     5521 tagctatcaa agtaggaaaa cgaccattac aaggaacaaa gttgtttgta agtatagcta
     5581 taagtctttc ccttggagag tcaattatcc tacaaccaat aactccagct gcattacatc
     5641 caaatcccat acacatagtc aaacactgtt ttccatgtgc acaagctttt ttaaataggt
     5701 ggtctaagtt aaatgcaact cttggcaaat atccaaaatc ctcaagtagg gtaaataatg
     5761 gaaaaaatat tgccatagga ggtagcatta cagagattac ccatgctaat gttctgtata
     5821 atcctaaaac caacatatca tttaaccatg atggacaatt aatactattt aatatacccg
     5881 aaatacttgg ctcaaatccg agtaataaat tagataataa agtggatgga tagttagccc
     5941 cttctatagt aatccacaat atagtaccta ataataagag cattattgga agcccaaata
     6001 ttttagatgt taatatttta tctacttttt cttctctatc agttgatttt ttagcaacat
     6061 tagaacaaca ctcatcactc agttttttag cataatcata gttgatttta gtaaactcat
     6121 ctcttatttt ctgtttattt atattgtctg gaatcttttt ctttacttca ttaatagcat
     6181 ctatagaatc tttgtctata taattactca tagattcaaa tatactttca tcgccatcaa
     6241 taagtctaag tcccaaccat ctagaattta tacctggtat aatattatcc aattctggtt
     6301 gaattgattt taccacattc tctatatttt cattgtatct aacaggttta ttatttaatt
     6361 tgtatttgtc aaaagataca tcatttaaag tatccaatag ttcatccata ccagagccat
     6421 ttcttgcagc agttaaaata actggaatac caagagaatc ctctaaaagt tttttatcaa
     6481 tagtaatgcc tttttttcta gcttcatcta ttaaattaat acacaaaata actttatctg
     6541 taagttccat cacttgaaat actaaattta aatttctttc aagacatgta gcatcacata
     6601 ctactattac tgcatctgga ttaccaaaac atataaaatc acgagctaca atttcttctt
     6661 gtgataaggc aaataaagaa tatgttcctg gtaaatctat taatgcatat tcggtatttt
     6721 tatatttaaa attaccacga gctgtagcta cagtttttcc tggccagttt ccagtatgct
     6781 gtctaagccc tgtaagatga ttaaatacag tacttttacc tgtatttgga ttgccagcca
     6841 atgcaattac aaactggtct tccttattgt ctatatcaaa catatctttt aaagaactca
     6901 ttttagttga gttatgtgtc aaccccatat taatatcctc ctgctagaaa tttttaaatg
     6961 tcagaaacta atattaagct actttcttct tgtcgtagag ctatcttact acctctgatt
     7021 ttatagacag ttaaattatt ttttggacct tttctaacta catctataac agctcctcta
     7081 gtaagaccaa gcgctaacat tctttctctt aaattaccac tagataatat atcttcaact
     7141 ttgacagttt ttttcacttg aatatcgtta aggtttttca tagtagcacc tccatgcttg
     7201 aaatttagcc tgggctaatc ttcttattct aaactatgca ttatattatt accttgttac
     7261 ttttacaaaa aaaagatagg caagatgcct atcatacaaa gttgggtata attcttactt
     7321 ttggggaaag tagaatatag aataaaaatt aacactttga atccatataa ttattgaatt
     7381 ttgataataa aaaagtcaga aagcaaaagc ctcctgactc tatgtaaggt attttcaaat
     7441 acctaaatat caattcaata tttaatttat acttaaatta tatattaaca atattagctt
     7501 gtcaacacta taatctcatt ttaatatgtt caatacctgc ttcgtcgtat acttctgaga
     7561 tttctttaaa accacaagat aagtaaaggt tctttatata agtttgtgct gataaggtta
     7621 tattattttc gtgtaagtta atttttataa aatctatagc acaatccatc atttgttttg
     7681 ctaatccttt tcttctatga ctagaaagaa ctaaaactct accaatggaa acatcattat
     7741 aagagctgta ttctttagga attattctag cataagcaca aatcaatcca ttttctttta
     7801 aaaatatgtg ataagagctt ttatctatat catcataatc gtttaatgaa aatatcttct
     7861 gttcacaagc aaatacctca tatcttgatt ttgctatttc ataaaattca tctaaactca
     7921 aatcattaaa gtgtttaatt ttaaacatag taattacctc ctagcatctt aaaaaactga
     7981 atattaagat acattatacc atttttaaca taaatacctt tattaaaatt tatatttatt
     8041 aaaaatattt ttaaaaagtg atatactata ttaaaaacaa aactttagtt agaaaattta
     8101 tgatttatgg aggcagtgtt ttgaatacta ctaaaaaatt aactgaagca gctttattgt
     8161 catctttatt catagtagta acaattatag ctgtgagtac tggatttgga tatgctattt
     8221 acttagattt tatagtgcca gtattttttt gtataatctg cttgaaatgt gatttaaagt
     8281 ataccatttt atctggaatt acttcacttt taataatttc tctggtactt ggaaatctag
     8341 gaactgctat ttgggcaagt cagagtgtat tacttggtat aatgtgtggc tatctaataa
     8401 ataagcctac aatggttatg gatgatttag tatatggttc agtatttggg gttattgtaa
     8461 tggttttcat tgatatatat gcttcaacat taataggtta tagttttatg aaagaatttc
     8521 aaggatatag caaatggatt tattttaatg ggtatacaaa ttttatttat tatttaatga
     8581 tagcattgtt tccttttggt atggtgtttt gcatatattt attaagttta attttaggga
     8641 ataaattgca tattttagat tctaattctt taaaaaaatt tttaatattt aagaatttta
     8701 gaagtttaaa ccaattttta tgttgctcaa aaaaagcttt ttacatatgt gtttcttatc
     8761 tagccatttt tgaagttcta aaactgttga atgttaaagt tgattctgtt tatttaaaaa
     8821 caatctttat atcaataacc tatataagtg tctattttat cattagagac tcatgtatgt
     8881 cactacaaaa ctttataata gctaagttta gaaaattatt atatgctaga attttatttt
     8941 tgataataat tttattgctg ttttttatat ttgatgtaac agttattagt ttaataatta
     9001 taaacgcatt tttaaataag aaaataaata taagattgag tcagagtaat atagtcaata
     9061 aacaaataaa tcttttgatt gaaaaataaa ctttgggtat tggtaaaaaa tataaaaaag
     9121 tagtataatg taaaaaaaca tgtatataat aataaaaaag gttgacaaaa tataaaaata
     9181 ataatatatt atgagtaagt taagaaaaat ttcttgtaat tgatgaaata atctgaatat
     9241 ataaatgcta tgaagagaag aagtagggct agattttatt taacagagag ttccagtttg
     9301 gtgaaatgga ataaattgat ttagtttgaa tacatctcag agcactatct ctgaaagata
     9361 acaaccatta agagatatcg gattagtaca cgttatagta cacgagtata tgtaattgta
     9421 ctctaagaga ttaatatcgt gagatattga tgaactaagg tggtaacacg taagcaatgc
     9481 tttcgtcctt ttaagaggat gaaagctttt tttattataa aaaaaaaatt atagattata
     9541 taaatatttt aggaggaatg aaaaatgaaa tcgatagacg agcaaatgcg aataattatg
     9601 aaaggtgtag atgatttaat agatgagaaa gagttaaggg aaaagcttat taaatcagaa
     9661 aaagaaggaa agcctatgat agtaaaattg ggtctagacc caagtgctcc agatatacat
     9721 ttagggcata cagtagttct tagaaagatg aaacaattac aagatttagg tcatcaaata
     9781 gtaataataa ttggagattt tacaggaaaa attggagacc caactggaaa atctaaagct
     9841 agaaaagctt taacaacaga gcaagttctt gcaaatgcaa aaacttatga agaacaaatt
     9901 ttcaaagttt tagataaaga aaaaactatt gtaagattta atagcgagtg gttagccaag
     9961 ttaaattttg aagatgtaat aaaattagca gcaactatta ctgttgcgag aatgttagaa
    10021 agagaagatt ttaaaaagag atatgaagga caaatgccaa tatcagtaca tgagttcttc
    10081 tacccattaa tgcaagcata tgactctata gcattagaag ctgatataga acttggtgga
    10141 actgaccaaa gattcaattt actaatggga agatcattac aaagagaatt tggtatggag
    10201 tctcaaatag taataatgat gcctttaata gaaggattag atggtaaaga aaaaatgagt
    10261 aagagtttag gtaactatat tggaatagat gaagaagcgg gaataatgta ccaaaaatct
    10321 atggaaattc ctgatgaact tataattaaa tactacaatt tagtgacaga tgttcatcct
    10381 gatgaagtta ataaaataga atctcagttg aaagagggtt cagtaaatcc aagagatata
    10441 aagatgaact tagcaagaga aatagtaaca ttataccatg gagaagaatc tgctaaagaa
    10501 gctgaagaaa gatttaaatc tgtattccaa aaaggtcaaa tacctgaaga tatacaaaca
    10561 attcaagtta aggaagatgg atttgactta atcgaagtac tagtttctaa tgagattgtt
    10621 aagagtaaga gtgaagttag aagattagct tctcaaggtg gagtaaaggt aaatggtgaa
    10681 aaagtagagg atttaagcac tattgtaaaa gagagtgaat tagttgttca aataggaaag
    10741 aaaaaatttg taaaaatcga gctagtaaaa tagagtaaag taaaagcaag tctattgagt
    10801 ataaaatgta cattcaataa acttgctttt ttattatatt aaaattttaa attgataacc
    10861 attgtcttta aaatatgtta taatttggtc caatgcattt actgtttctt gttttccata
    10921 agtatcatgc attaaaagaa caaccatttc tttgccttca gaagatttct ttgcatgttc
    10981 aaataattct tgtgcatttt tctttttacc ttctgcatca gcatttaaag cgttccaatc
    11041 aattgatgcc atgtttttct cttttaagta gtttcctaga ggttccatat ttttccatga
    11101 catatacccg cctgggcatc taactacatt tgaggaaaaa ttctttccta agactttttt
    11161 catagcttca tcagttttgt taagttcatt tacgaatgta tcgagattta ggcttctgcc
    11221 aggatatagt tttttatagt catgggtata agagtgatgg gctattgcat ttccttcgtc
    11281 aaatgttctt tttaaaattt cattagctcc ttttctttca agagaatcac ccttaataaa
    11341 gaaagttcct ctaacaccat gtcttttcaa gatatctaga acttgtggag tattagtagt
    11401 agatggtcca tcatcaaatg taagaaagac tatcttttct ccattgtttg aatagtcgta
    11461 tttgttaagt ttgtcccaaa ctatttttgc gtcatatcca tattctttta cagcttcttt
    11521 ttttgcacca ataataggtt catttttttc tttttcaatt ctttcttttt ctaatctttc
    11581 agctttaagt ctttcttgag tttctttcat ttcttttttt ctagacatgt ccgcccatgc
    11641 catagaaaaa ataccaataa ctattacaac aagtatagta agtgtaacag ttttcttttt
    11701 tctttttttt accataatta cagcattccc ccttttgtcg aattgtatca aaaatatatt
    11761 ataattatat cgaagaaagt agaaaaagta aaccgacaaa gttgtaacta agataactaa
    11821 agtgtaactt atatgacatt atttgca
//
LOCUS       DAMJVX010000109        11714 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMJVX010000109 DAMJVX010000000
VERSION     DAMJVX010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11714
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.113"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..391
                     /locus_tag="PC407_002548"
     CDS             <1..391
                     /locus_tag="PC407_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2497985.1"
                     /translation="AKDLKKVYAAITEEVALSELSLLEEKWGNQYAIALRSWRSNWNE
                     LATYFKYPQEIRTLIYTTNSMESYNRLLRKVTKSKSIFPSDDSLHKSLYLATMDISER
                     WTQKIRNWTQILAHLSIYFEERILTLN"
     gene            868..1479
                     /locus_tag="PC407_002549"
     CDS             868..1479
                     /locus_tag="PC407_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497986.1"
                     /translation="MNIKRINLLLSTVMAAVIISLPLAVTANAMEKTESDNNVKIELS
                     DKEVEELHNYWPEDKIFSDEKIDEILIKTGQFTQKELDKINQEVDDSAKKVNQNSRVK
                     YINGYNKYRTFTKNGKNYLYIYVSGKTLKKIKAGTDLASTFGGFLPKIWVELDVVAIG
                     KIITINMDGINTNYGIVLSYIEDKWNFQGATYTYRYDHWFYQT"
     gene            1507..1689
                     /locus_tag="PC407_002550"
     CDS             1507..1689
                     /locus_tag="PC407_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497987.1"
                     /translation="MKKRLYKVFLKTIPNLIIVILSLALSFYTNTFDIDLKILLLIPL
                     AYVLLFIYYYIKTKKD"
     gene            1929..2471
                     /locus_tag="PC407_002551"
     CDS             1929..2471
                     /locus_tag="PC407_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2497988.1"
                     /translation="MLIYLAILELEEDKIKFEKVYEKYRQIMFYVANKILKDDHLSED
                     AVHNAFLRIIKNIDKIDEVDSPRTKAFIVIIVERIAIDFYRKRKREKVSDIEEEYKNR
                     EINFSIEDKVCESNLAIALAKLNESYFQVLSLKFQYGFSNKEIANALNLGEENVYKRI
                     QRARKKLKEILEEMEVGNIE"
     gene            2503..3165
                     /locus_tag="PC407_002552"
     CDS             2503..3165
                     /locus_tag="PC407_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HDF2497989.1"
                     /translation="MLRESIPIATKIIIDDLLSDDELNHVFSKGFERKMKKLIKQQKR
                     SSLTNKIIFYSKQTAIVFLIVLASLLTITMSSEALRSRFFRLVIRVYEDLTSFVFSTN
                     GEDNLSLKNKEPEYIPKGFKEVDRVDDFVITYRNDENIEIQYVTDKISSNSIILDTEN
                     AKVKDIFVNGHKAKYIVKGTFLQLFWNDNNFIYLLNVDYQNSGESEFYEDILIKIAKS
                     IK"
     gene            3355..3672
                     /locus_tag="PC407_002553"
     CDS             3355..3672
                     /locus_tag="PC407_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497990.1"
                     /translation="MKSDLYLCKSNKLYRDNEVLEKFMEDLSFLIDDYKFSLETISIL
                     CKINIEKLTNFYAGKGYILYEEMSLIESVLAPFLSAIDIAKHNYDVVIKSRESDKPLI
                     FEN"
     gene            3694..4137
                     /locus_tag="PC407_002554"
     CDS             3694..4137
                     /locus_tag="PC407_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497991.1"
                     /translation="MKRLFALLLTLSLVILPSTLVFANEKAENDSIYAEAPLVFQSNE
                     KAYTHGSGGTAYIGYMSSKHLNWRITSSKGIVILFSGTVEIRNSKNMLVASYPISGGG
                     SSTISGQIDVKYLKKGDYKAILKGTSTTGGGVLVVKSGLVQMFTI"
     gene            4355..4798
                     /locus_tag="PC407_002555"
     CDS             4355..4798
                     /locus_tag="PC407_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497992.1"
                     /translation="MNLRKTVISGMCALVCSSAVAIPAFADEYQDKNIEQYSAVNYID
                     DTMVKMSYIKSGTCNLSISGSTAKVTCFVRGASNVTSTSIIARLQKSNDNGKTWSTIQ
                     TWNASGNISCSLSKSKTIGKGYYRVHSTIKANSESKNIISSTKKY"
     gene            complement(4929..5349)
                     /locus_tag="PC407_002556"
                     /pseudo
     CDS             complement(4929..5349)
                     /locus_tag="PC407_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            complement(5327..6604)
                     /locus_tag="PC407_002557"
                     /pseudo
     CDS             complement(5327..6604)
                     /locus_tag="PC407_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(6627..7169)
                     /locus_tag="PC407_002558"
     CDS             complement(6627..7169)
                     /locus_tag="PC407_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497993.1"
                     /translation="MLLCDPASAGGSKHDYSAFLVGSESENGLLCGRLAELAKINART
                     DFDKYILHMIYLLKVYPDITHVYIEKNTFNGTAANQLELKIKNDDVLYYRDIEIINEH
                     QKKNKDDKISTLIPVLNKGQMIFAEEDKAFIQQILNFTGQKYSLHDDAPDISAEFINR
                     IFNIKVNESITLLDRRNLGL"
     gene            complement(7209..7427)
                     /locus_tag="PC407_002559"
     CDS             complement(7209..7427)
                     /locus_tag="PC407_002559"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497994.1"
                     /translation="MLFDFKNTNHLEDAKEFYWENEKEIKFPILWPSFWNCLDMALSY
                     YENPTSFKQEVQNDVNSIGEKWFKTIRT"
     gene            complement(7623..8366)
                     /locus_tag="PC407_002560"
     CDS             complement(7623..8366)
                     /locus_tag="PC407_002560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497995.1"
                     /translation="MDNLERNIQLIFSYMAKTFIRNGATIREAEKEANEIIKTNSSNL
                     WGVNGLAYQLGKINLEFFCMYFIQDTYLPKEDNVAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGKKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKKFSDDVKYAA"
     gene            complement(8666..9223)
                     /locus_tag="PC407_002561"
     CDS             complement(8666..9223)
                     /locus_tag="PC407_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2497996.1"
                     /translation="MVVGYMRVSTAEQNEDRQLVTMKKHNVEKMYQEKISAKDTKRSK
                     LNAMLDFVREGDTIIVHDFSRLARSTKDLLEIVELLEKKHVKLISDKENLDTSTPTGK
                     LMLTMLGAIYEFERTNMLERQREGISIAKEQGKYKGRKEVKIDNFGSYYDRYKTRELN
                     KTQLAKELGVSRPTLDRLIKSHESN"
     gene            complement(9418..9606)
                     /locus_tag="PC407_002562"
     CDS             complement(9418..9606)
                     /locus_tag="PC407_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497997.1"
                     /translation="MNNKKKSFLISGTINVILLIIVFALFSYNIIENSTRNMIITVIL
                     IFIFEIIKIKILEKYYNL"
     gene            complement(9617..10192)
                     /locus_tag="PC407_002563"
     CDS             complement(9617..10192)
                     /locus_tag="PC407_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2497998.1"
                     /translation="MLKKKIISFLMAGIFVLSSGYNLVYASTFEENEYKQELVNENEF
                     TIINSNENEMIVDKKGNNTYVSTKVTPGSWKNVKYLGQKSRKLSSPYTRERIIQTIQW
                     GLGYLSGGSSFLLGLAESIYREKVYDITKYGGVYTKTYYRRQITDIRNSHANVPVPYR
                     YEYVTYFYSSSNFKKVVYTERVGDGIKLANN"
     gene            complement(10525..11664)
                     /locus_tag="PC407_002564"
     CDS             complement(10525..11664)
                     /locus_tag="PC407_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2497999.1"
                     /translation="MVIKLTIEEYKNKYSTGDKIILSLKSFDNKELYYHETDNILNSK
                     DEIDRLTSSKIFKIIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYNLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLDPLKIYNFMDELIIFMENSKVNDLVKSTIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLINKGYDTFKEFSISYIISKNRTKYYKAILDVENKGYNLTVFIEFMLKSIIQSINEM
                     RDMHDRKSLESILKEELFENDITLSATEEHILKYICSKDNYSMTLENYIKKNKGRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSREKDVYKVNEKYLNMLEIS"
BASE COUNT         4174 a         1585 c         1440 g         4515 t
ORIGIN      
        1 tgcaaaagat ttaaagaaag tatatgctgc aattacagag gaagtagcat taagtgagct
       61 atcgctctta gaggaaaaat ggggtaatca atatgcgata gcattaagaa gttggcgaag
      121 taattggaat gagctagcaa catactttaa atatcctcaa gagataagaa cattgatata
      181 tactacaaat tcaatggaaa gttacaatag attattaaga aaagttacta aatcgaagtc
      241 tatttttcct tcagatgatt ctttgcacaa atcattatac ttagcaacta tggatatttc
      301 tgaaagatgg actcaaaaga ttagaaattg gactcaaatc ttagctcatt taagtattta
      361 ttttgaagaa agaattttaa ctctaaatta ataaagaaat atttcaaaaa aattaagaaa
      421 ttttcttaaa gggataataa tttttacaaa aatggaaaaa atatcaaaat aaggctatga
      481 tttttaaagc catagcctta ccaataaatg tttaattatc actaaaaaaa taaattttta
      541 ttcaaagaat acacataaat atttccagac tcacttattg tataagttat attctatttt
      601 gtatgaagtc aattaaaaac tgaactatat taaaattaat tttataaata acaaatataa
      661 ataattacaa aaccttaact gcgttaagtt tgaaatataa taatattaaa aacacttctc
      721 tcaaatcaag tatcttttgt tatattttca taagttttaa aaatataaat tttttaaaac
      781 ttgtgtccaa aaaacaaaat taaatatgtt atatatgtaa agggttaatt ttaaaacctt
      841 aaatttatta tagttagggg gatttaaatg aatatcaaaa gaattaatct ccttttatca
      901 acagtgatgg cagctgtgat aatatcttta ccacttgctg ttacagcaaa tgcaatggaa
      961 aaaacagaat cagataataa tgtgaaaata gagttgtctg acaaagaagt agaggaatta
     1021 cataattact ggcctgagga taaaattttt tcagatgaaa agattgatga gatcttaatt
     1081 aaaacaggtc aatttactca aaaggaattg gataaaatca atcaagaagt ggacgattct
     1141 gctaagaaag taaatcaaaa ttctcgtgtg aaatatataa atgggtataa caaatacaga
     1201 acttttacta aaaatggtaa aaactattta tatatttatg taagtggaaa gacattaaag
     1261 aaaataaaag ctggaacaga tcttgcatct acttttggag gttttttacc aaaaatttgg
     1321 gtagagcttg atgtagtggc aattggaaag ataataacaa ttaacatgga tggtataaat
     1381 actaattatg ggattgtttt atcatatata gaggataagt ggaattttca gggtgcaaca
     1441 tatacttata gatatgatca ttggttctat caaacttaaa agacatgctt ttttaggagg
     1501 aataatatga aaaaaaggct ttataaggtt tttttaaaaa caattccaaa cctgataatt
     1561 gtgatattat cacttgcatt atcattttat actaatacat ttgatattga tttaaaaata
     1621 ttgcttttaa tacctttagc atatgtactt ttgtttatat actattacat caaaacaaaa
     1681 aaagattaat ttttatggaa ttagatatat acgaaaataa ttaatttaaa aatttaactt
     1741 tgagaatcat ttgtagagtt aaggccctta taatcccgaa aatggatttg aagggccttt
     1801 ttactagata tctttaaaaa gcttgttaag catagttata ttctatttaa gtagagttaa
     1861 ttatatattt atgttataat tgaactttaa aatattaatt cagaattttt atattggagg
     1921 aattctaaat gttaatttat ctagctatac ttgaactcga agaagataaa ataaagtttg
     1981 aaaaggtata tgaaaagtat agacaaataa tgttttatgt agccaataaa atattaaagg
     2041 atgatcattt atcagaagat gcagtacata atgctttttt aagaataatt aaaaatatag
     2101 ataaaattga tgaggtagac agtccaagaa cgaaggcttt tatcgttata atagtagaaa
     2161 ggattgctat agacttttac agaaagagaa aaagagaaaa ggtatctgat attgaagaag
     2221 agtataaaaa cagggagata aatttttcta tagaagataa agtatgtgaa agtaatttag
     2281 ctatagcact tgcaaaactt aatgaaagtt actttcaagt gctttcctta aagttccagt
     2341 atggtttttc aaataaagaa atagcaaatg cattaaattt aggggaggaa aatgtatata
     2401 agagaattca gcgtgctaga aaaaaattaa aagaaatatt agaagaaatg gaggttggaa
     2461 atattgagta ataaaaaaga tgattttgat attacggatg atatgcttag agaaagtatc
     2521 ccaatagcga caaaaattat tattgatgac cttctttcag atgatgaatt aaatcatgtt
     2581 ttttcaaaag gttttgagcg aaaaatgaaa aaactcatta agcaacaaaa aagaagtagt
     2641 cttacaaata aaatcatttt ttattcaaaa caaacagcta ttgtattttt aatagtttta
     2701 gctagtttac ttactattac tatgagtagt gaggcattgc gttcacgttt ctttagatta
     2761 gtaataagag tatatgaaga cttaacttca tttgtttttt caactaatgg agaagataat
     2821 cttagtttaa aaaataaaga acctgagtat ataccaaaag gatttaaaga agttgataga
     2881 gttgatgatt ttgttattac atatagaaat gatgaaaata ttgagataca atatgtaaca
     2941 gataaaatta gttcaaattc tattatatta gatacagaaa atgcaaaagt taaagatata
     3001 tttgtaaatg ggcataaagc aaaatatata gtaaaaggaa ctttcttaca gttattttgg
     3061 aatgacaaca actttatata tttattaaat gtagattatc aaaatagtgg agaatctgaa
     3121 ttttatgaag atattttgat aaaaatagcg aaaagtataa aataattttt ttaagtgtcc
     3181 aagggtagcc tcttcattcg ttatacaagt atatatgaaa atgtgaggtg atttttatgg
     3241 ttttaaaatc tacatttacc tgagcgttaa aaatattgtt ataggaaaaa ttaatacatt
     3301 atagttataa catgcttgca ataggaataa aactttaaaa ttgaggtgta aaaaatgaaa
     3361 tcagatttat atttatgtaa aagtaataaa ttgtatagag acaacgaagt tctagaaaaa
     3421 tttatggaag atttaagttt tcttatagac gattataaat tctcgttaga aacaatatct
     3481 attctatgca aaataaatat tgagaaatta actaatttct atgcaggaaa aggatatata
     3541 ctgtatgaag aaatgtctct tattgaaagt gtacttgcac cttttttaag tgcaattgat
     3601 attgctaaac ataattatga tgttgtcatt aaatcgaggg aaagcgataa acctttgata
     3661 ttcgaaaatt aatttaaaac tgggggaata cacatgaaaa gattatttgc gttattactt
     3721 actttaagtt tagttatatt accatcaact ttagtatttg ctaatgaaaa agctgaaaat
     3781 gattcaattt atgctgaggc gccattagtg tttcaaagta atgaaaaagc atatacacat
     3841 ggtagtggag gaactgcata tataggttat atgtcaagta aacatcttaa ttggaggata
     3901 acatcttcaa aaggaatagt tatactattt tcaggaactg ttgagattag aaattcaaaa
     3961 aatatgcttg tagcatcata tccaataagt ggtggaggat cttcaactat atctggacaa
     4021 atagatgtta aatatcttaa aaaaggggat tataaagcta tattgaaagg tacaagtaca
     4081 acaggaggcg gagtcttagt agtaaaatct ggtctagttc agatgtttac aatttaatat
     4141 tataagtaaa ataaaaatca tgaatttatt gggggatagt cacaatacat attatttaca
     4201 gatattcata actattatag ttaaagacat tctattataa agaaaatgta taatttaaaa
     4261 ttataataaa aaaaattatc attagtgtcc aaaaatcact tcttcattcg ttatataagt
     4321 tatataaata gaaaacaaat gaagaggtga ttttatgaat cttagaaaaa cagttataag
     4381 tggtatgtgt gctttagtat gttcatcagc agttgcaata cctgcatttg ctgatgaata
     4441 tcaagataag aatattgaac aatattcagc agtaaactat atagatgata ctatggtgaa
     4501 aatgtcttat attaagtctg gtacttgcaa tttaagtatt tcaggaagta cagctaaagt
     4561 aacatgcttt gttagaggag catcaaatgt tacaagcaca tcaattattg ctagattaca
     4621 gaaaagcaat gataatggga aaacatggtc aacaatccaa acatggaatg cttcaggaaa
     4681 tatttcatgt tctctttcta agtcaaaaac tataggaaaa ggatattaca gagttcattc
     4741 cacaataaaa gctaactctg aaagtaaaaa tataataagt agtacaaaga aatattaaaa
     4801 atagatattg tatttatgag ttgtagtaaa caagaataat agttttttaa aagcaaatat
     4861 aattaatttt aaatagagtg ccaatataaa ataggtgtat aatatttata cttttttata
     4921 ttggcactct attttataaa ataagttatt atagaaaatg aaacataatt tatatataaa
     4981 ttttctgttt gacctggtat ataatctttt atatcacata tcagtatttt cccatcatta
     5041 taaccccaac caatcacagt tgcaggtatc atatcagctt ctccatcata aatgattgta
     5101 tgtttataca tgatttttcc ctcgctatta tctatagttt tattcaaaat accttctgct
     5161 attaatttag caactatgtc tttaatagtc tgtatctgct ttactgtcta caaaacatac
     5221 ttcaattaat atcgctggag cttttgtatg actaagccaa tacaattccc ttacgtccga
     5281 ttttgcacct ctattttcag cagatttata tatatcttca gtcaatttag ttataatctt
     5341 ttgattcatc taataaaatc tctccctctg aaactatttc ctgttctttt ttagcctttt
     5401 ccatttctgc atgaggatta tcaataaaac ttggttgaga taatccggtt tcagtagata
     5461 atttatctcc aagttgactt aatatatttg ctacaactaa atcattatgt ggtatcttag
     5521 gggtaaattt agccttaata tctttataat catagtcaaa accatatttt ttattaagcc
     5581 aaatgcacaa taatttgtac ctattaaata aagtatctgc aatactatca ttattctggc
     5641 tacaaatcca ttctgtactg attaatcttg ctacaactgc taaacttgat gtattacttt
     5701 gcattttttc attgtgattt atatgagatg taattttata tatgttttct tttattgaag
     5761 atagtgtatt ttgaacaaat gtatcattca tgtctttaat aagccattct atttttccat
     5821 ctccattaac ttgaagtatt ccaagttctt tcatccttgg caaatcatct tctttaatat
     5881 tacattcaga gaaggttaaa taagcatttc taaaatcact aatttcatta cttatatcac
     5941 ttaaattagt ttcataagca tcttgtaatc cttttatatc attgaatatt gtattttttc
     6001 ctagttcttc acttctttta caaattgata ttggaacatt accattaaat atattattta
     6061 ctgtaggttt ttctatctct ttaaaatttg aatcaaaatg atatatgtat tctttatcat
     6121 atacatctat ataaatatca tctttaaaat cttttttaaa ttctctaaaa aaatagatgg
     6181 tctctccatc ttcattttca taatggcagc catctgttgg ttttattatt cttactttca
     6241 tttcattgtt ttttacatag taaagttcat agcaaaatcc aaataatagt gtatatctca
     6301 ataaatctga atcgtgcttt ttactccaat ggcaagtata gtattctaaa tcatttataa
     6361 tcttctcatc acctaatcta cttgtatagg tgattttatt tgcaagagaa taagcgacct
     6421 cttcattaat aaactttttt aagaaattag tgtttatctt attattagac ctcttggtaa
     6481 ccattttata gttactaata gcatctgttt cacctttata atacttatac atagtgttgt
     6541 attcttgctt ttttaaactc tagtcttcat atatttctct taataaactt aaatctattt
     6601 ccatttaatg catcacctcc atacaatcaa agacctaaat ttcttctatc taatagtgta
     6661 atactttcat ttactttaat attaaaaatc ctatttataa attctgctga tatatctggt
     6721 gcatcatcat gtagagagta cttttgtcct gtaaaattaa ggatttgctg tataaatgcc
     6781 ttatcctctt ctgcaaaaat catttgacct ttatttaaaa caggtattaa agtagagatt
     6841 ttatcatctt tatttttctt ttggtgctca ttaatgattt ctatatctct gtagtaaaga
     6901 acatcatcat tttttatttt aagttctaat tgattagcag ctgtaccatt aaatgtattt
     6961 ttttctatat aaacatgtgt tatatctgga tacactttta ataaataaat catatgcaat
     7021 atatatttat caaaatcagt tctagcattt attttggcta gttctgccaa tctgccacat
     7081 agtaaaccat tttctgattc acttccaaca agaaaagcac tatagtcatg tttagaacca
     7141 cctgcagatg ctgggtcaca taataacatt gtctttttga atgtatgagt ttctatacat
     7201 tctctagatt aagttctgat tgttttaaac catttctcac ctattgaatt tacatcattt
     7261 tgtacctctt gtttaaaaga tgttgggttt tcgtaataac ttaaagccat gtctaaacaa
     7321 ttccaaaaac ttggccataa tataggaaat ttaatttctt tttcattttc ccagtaaaat
     7381 tctttagcat cttcaagatg atttgtgttt ttgaaatcaa ataacaactc tttgaaatta
     7441 agccataaac cagaattaaa taattcatct acattatcaa ctaaaactcc tttttcacat
     7501 ttaaatttcc aagttggtaa attctttagc ctactataga aacattcttt atgttgtaag
     7561 gttcctaatg ctataaaagt tgttcctctt tttataatct taccatttct tattactggt
     7621 ttttaagcag catattttac atcatcagaa aattttttaa attttttctc tctagcatct
     7681 tctgttctaa cattttcttc tgactgataa tcatcaagta tgataagatc tggacgatta
     7741 ttattgtatt tttttcctct cattggtgat gaagatgata ttgcttcaat aaaggtttta
     7801 ttagttaatt ctaattgagt actattgcaa atatattttc tatcattatc atttaagaga
     7861 actccaaatg cttttttaat atactcattt tcaagtaatg cattttttat atcttttacg
     7921 aatttttctg ctgtagagcc tatatcagag caaattaaag tatatgtctt atgtttataa
     7981 caatgtgacc atacagttgt tgctaaatct ccaaatgcac ttttacctgt tcctcttgga
     8041 taaattcttc ctaattgctg tgaaccatct ccaattatag actcttgtat atcttcccat
     8101 aactcatgat gaactttagc tataggagca gcaacattat cttcttttgg taaatatgtg
     8161 tcttgtataa aatacataca gaaaaattct aaatttattt ttccaagctg ataagctagg
     8221 ccattaactc cccaaagatt gcttgaatta gtttttataa tctcatttgc ttctttttca
     8281 gcttctctta tagttgctcc atttcttata aaggtctttg ccatataact aaatattaat
     8341 tgtatatttc tttctaaatt atccactcgt atttacttcc tcttaattct tctatttttt
     8401 taggttcagt gagtaataca attaataaaa taaacaatgc aaattttttt ctaataaaat
     8461 caccctcttt gaaatatgtc tctggttttt aatttttgct agaaaatttc tagaactcat
     8521 tttctataaa taatactttt ttgaaataga aggatacccc ctatactgaa aaacagttat
     8581 ttataattat tttaaacata attaaagggc ataattctat gaatttgccc cactaaaatt
     8641 aacttaagca tatatattgc tcttcttagt tactctcatg gctctttatt aatctatcta
     8701 atgttggtct acttactccc aattctttag ccaattgagt cttattaagt tctctagttt
     8761 tatatctatc ataataacta ccaaagttgt ctatcttaac ttctttacgt cctttatatt
     8821 taccctgttc tttagctata gagatacctt ctcgttgtct ttctaacatg tttgttctct
     8881 caaattcata tattgcgcca agcattgtaa gcataagttt tcctgtaggt gtacttgtat
     8941 ctaaattttc tttatcacta attaacttta catgtttctt ttctaagagc tcaactattt
     9001 ctaataagtc tttagtactt ctagcaagtc tactaaagtc atgtacaatt attgtatctc
     9061 cttctcttac aaaatctaac attgcgttta gttttgatct tttagtatct ttggcactta
     9121 ttttctcctg atacattttt tctacattat gctttttcat tgtaactaat tgtctatctt
     9181 cattttgttc tgctgttgaa actctcatat aacctactac catctttgat acctccacct
     9241 atgtttactc tataaataat attatagcat aaatgtaaaa ctagagttaa agatttgcca
     9301 tgctttttgt aaattaattg gagttttgat tttattttta catcagaata gatagtttgt
     9361 aatgtaaatt tgtaccagca tactttacaa tatgctctta ggaaagatat ttcttgttta
     9421 tagattataa tatttctcta gtatctttat ttttataatt tcaaaaataa aaattaaaat
     9481 tacagttatt atcatatttc gtgtactatt ttctataata ttataactaa acaatgcaaa
     9541 aacaatgatt aataaaatta cgtttatagt gccagaaatc aagaatgatt ttttcttatt
     9601 attcatatat ttcctcctag ttatttgcta gttttatacc atctcctact ctttcagtat
     9661 atacaacttt tttaaagtta gatgagctat aaaaatatgt aacatattca tatctgtatg
     9721 gaacaggaac attagcatga ctgtttctaa tatcagttat ttgtctccta tagtatgttt
     9781 ttgtatatac gcctccatat ttagtaatgt catatacttt ttctctataa atactctcag
     9841 ctaaacctaa taaaaaagat gaccctccag aaagataacc taatccccat tgaatagttt
     9901 gtataattct ttctcttgta taaggagaac ttaatttgcg tgatttttga cctagatatt
     9961 taacattttt ccaactacca ggtgtaactt ttgttgaaac ataagtgtta ttgccttttt
    10021 tatccacaat catttcattt tcattgctat taataatagt aaattcgttt tcattaacaa
    10081 gttcttgttt atattcattt tcttcaaaag tagatgcata aaccaaatta tatccactag
    10141 ataaaacaaa tattcctgcc attaaaaaac ttattatttt tttctttaac ataatatttt
    10201 actaccttta cttaaatatt tattctataa acactgtcat ataacctgtt agttatatta
    10261 aaaataatct ttttatataa cttcacaatc aaaccttgtc ttatttcata atagttttat
    10321 ttaatatcat ataatttaga cctccttata agattattta tatatataac gaataataaa
    10381 agtaattttg gacaaaataa aaatcttttt attttaagaa tgaaaaattt aaccctctaa
    10441 gaaaactcaa ttaagagcga ttcatagagg gcttattatt atattatata actttatata
    10501 ctttttattt ttatatgctt caatttaact tatttcaagc atattcaagt acttctcatt
    10561 aactttatat acatcctttt ctctagataa aatctctatt tcttcaagat tattaaacac
    10621 ttccatcaac tggtcaacta attcgatttc ttttattcca gcctttaagt atctaccttt
    10681 attctttttg atatagttct ctaatgtcat agaataatta tctttactac agatatattt
    10741 taatatatgt tcttctgttg ctgatagagt tatatcattt tcaaataatt cctcttttaa
    10801 tatactctct aaagacttcc tatcatgcat atcacgcatt tcattgatag attgtattat
    10861 agactttagc atgaactcta taaatacagt taaattatat cctttattct ctacatctag
    10921 tatagcctta taatacttag ttctgttttt agatattata tatgatattg aaaactcttt
    10981 aaaagtatca tatcctttat taattaaata tagatatgat aaggctcttg atgttcttcc
    11041 attaccatca ctaaaaggat gaacatatac aaagtagaaa tgaattattg tactctttac
    11101 taaatcattt actttactat tttccataaa aattattaat tcatccataa aattatatat
    11161 tttcaaagga tcaagtcctg tatgtataat ctcacctttt gaatcacata catatacttt
    11221 ttcagttcta tattcatatt cttctgaatc taaacagttt tcacccaata tatgatgtaa
    11281 attatatata aatttgtgac tatataattt atctaaatta tctaatccat attctaaggc
    11341 tctatgatta ttgtaaatca tatactcact tttattactt ggtttcatct ttcctctaat
    11401 tatagatttt gcaatctttc tagttgaaaa ggcaccttct attacactag aataataaga
    11461 ctctaaggct aaatcatttt tatcttctat tattttaaat atcttgcttg atgttaatct
    11521 atctatttca tcttttgaat ttaatatgtt atcagtttca tgatagtata gttctttatt
    11581 atcaaaactc tttaaggaaa gtataatttt atctccagta gagtatttat ttttgtattc
    11641 ttctattgtc agtttaatca ccaccttagt ttatctttat ctattaatat taacaaaaag
    11701 tattagtttt taca
//
LOCUS       DAMJVX010000110        11634 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMJVX010000110 DAMJVX010000000
VERSION     DAMJVX010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11634
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.114"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            54..314
                     /locus_tag="PC407_002565"
     CDS             54..314
                     /locus_tag="PC407_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498000.1"
                     /translation="MFKTVFEDKEYVLLCKNYSKSDGVKAIFIYLISMLLLFFQGYLY
                     TTNLSSTILTNSQMILSILILLIGLGFVLLSKEKLNTIGITM"
     gene            333..761
                     /locus_tag="PC407_002566"
     CDS             333..761
                     /locus_tag="PC407_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2498001.1"
                     /translation="MYGVIGATVLVIFVILTAGIMQGKKVDFQLPTVFMIVTFIVSAI
                     SEEIVFRGYIQTRLTGLIKNSVLSSCINAFLFLSTHYPVKWISSGDFSFAILSGFYVI
                     CLILLNFSCDLVYRKTNCLWGAWLLHILYNIGTGVLIFTT"
     gene            881..1489
                     /locus_tag="PC407_002567"
     CDS             881..1489
                     /locus_tag="PC407_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDF2498002.1"
                     /translation="MVDNLIKYIHKSNIAIVVLHEIYGVNNFIKDVCQKYYKGGYDVF
                     CPELLGEKRVFSYLKAQEAYDFFINNVKFDIYKKVEKLINGLKSEYKHVFVVGYSIGA
                     TIAWRCSENSLCDGIICCYGSRIRDYMDVIPTCPILLVFAKYDSFDVDYVSSQLSRRQ
                     NIQIEILDANHGFIDTYSKNYSNAQTEVFNIKEKNFLSKCLG"
     gene            2013..2225
                     /locus_tag="PC407_002568"
     CDS             2013..2225
                     /locus_tag="PC407_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2498003.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            2238..2459
                     /locus_tag="PC407_002569"
     CDS             2238..2459
                     /locus_tag="PC407_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2498004.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            2559..4715
                     /gene="feoB"
                     /locus_tag="PC407_002570"
     CDS             2559..4715
                     /gene="feoB"
                     /locus_tag="PC407_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2498005.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTIIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHVPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            4741..4893
                     /locus_tag="PC407_002571"
     CDS             4741..4893
                     /locus_tag="PC407_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDF2498006.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            5073..5783
                     /locus_tag="PC407_002572"
     CDS             5073..5783
                     /locus_tag="PC407_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2498007.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            5868..6638
                     /locus_tag="PC407_002573"
     CDS             5868..6638
                     /locus_tag="PC407_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2498008.1"
                     /translation="MFEKIHKRFKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVVFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            6631..7362
                     /locus_tag="PC407_002574"
     CDS             6631..7362
                     /locus_tag="PC407_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498009.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            7396..8355
                     /locus_tag="PC407_002575"
     CDS             7396..8355
                     /locus_tag="PC407_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2498010.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(8453..9049)
                     /locus_tag="PC407_002576"
     CDS             complement(8453..9049)
                     /locus_tag="PC407_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HDF2498011.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVNKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            9295..9894
                     /locus_tag="PC407_002577"
     CDS             9295..9894
                     /locus_tag="PC407_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498012.1"
                     /translation="MKLKRTLFVGLALVLAIGITACARDNEKPNENAGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKRMNDDLIAEGERLVKDIDERIEKLDKVSDEDMKKGQN
                     DFMKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            complement(10062..10529)
                     /locus_tag="PC407_002578"
     CDS             complement(10062..10529)
                     /locus_tag="PC407_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498013.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIEESYNFAKQKFKKRKNK"
     gene            10640..11551
                     /locus_tag="PC407_002579"
     CDS             10640..11551
                     /locus_tag="PC407_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2498014.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SVGFPLYSKVGAAGGYHVLKERILPPITFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENMRRKLVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSIRTIVPIGLYAMNGLWYCPAYCIKSESVKEFRADRIVKILSIENLSNKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKK"
BASE COUNT         4376 a         1250 c         2063 g         3945 t
ORIGIN      
        1 tatgataaaa tatgagaaat aaataagaat ttaattagaa aggagtattt atagtgttta
       61 aaactgtatt tgaagataaa gaatatgttt tattatgtaa gaattattct aaaagtgatg
      121 gagtaaaagc aatatttatt tatttgatat ctatgttact attgtttttc caaggttatt
      181 tgtatacaac aaatttaagt tcaacaattt taactaactc acaaatgata ttgtcaatct
      241 tgattttact tatagggctg ggatttgtac tactttcgaa ggaaaaacta aatacgattg
      301 ggattacaat gtgaaattta aataaaagtc ttttgtatgg agttattggt gctactgtac
      361 tcgtaatttt cgttatttta acagctggaa ttatgcaagg aaaaaaagtt gattttcaat
      421 tacctactgt ttttatgatt gttacattta ttgtttctgc tatttcagaa gaaattgttt
      481 ttagagggta tattcaaaca aggttaactg gattaataaa aaatagtgta ctttcatctt
      541 gtattaatgc ttttcttttt ctatctacac attaccctgt aaaatggatt tcaagtggag
      601 atttttcatt tgcaattcta tctggatttt atgtgatatg ccttatatta cttaactttt
      661 catgtgattt agtatatcga aaaactaact gtctgtgggg ggcatggcta ctgcatatct
      721 tgtataacat tggcacagga gtgctgattt ttactactta gtcaatttta ctttgcagaa
      781 agtttatcgt aagaaacgga ttattttata agtcaaattt tagagctagt aaattgacat
      841 acttaaaata atttaaacta gatgtaaaaa atatcgacag gtggtggaca atctgattaa
      901 gtatatacat aaaagtaata tagctattgt tgtactgcat gaaatttatg gagttaataa
      961 ttttataaaa gatgtttgtc aaaagtatta taaaggtggg tatgatgtgt tttgtccaga
     1021 actcttagga gaaaaaagag ttttttcata cttaaaagct caagaagcat atgatttttt
     1081 tataaataat gttaagtttg atatttataa aaaagtagaa aaacttataa atggtttaaa
     1141 atcggagtat aaacatgtat ttgtagttgg gtatagtata ggtgcaacta ttgcatggag
     1201 atgtagtgaa aactctttgt gtgatggtat aatttgttgt tatggctccc gaattagaga
     1261 ttatatggat gtaattccaa catgtcctat tttgctagtg tttgcaaaat acgattcatt
     1321 cgatgttgat tatgtttctt ctcaactttc tagaaggcaa aatattcaaa ttgaaatttt
     1381 agacgcaaat catggtttta ttgatacata ctcaaaaaac tattctaatg ctcaaacaga
     1441 agtttttaat attaaagaaa aaaactttct atctaaatgt ttaggctaat atgttttatt
     1501 ataaaatata gaaaatttaa tcaatttgaa aaacataatg acactactaa tgttaaaaaa
     1561 tattagtagt gtcattatat tttttgagat agagatagtc agtagaatca tagaattaat
     1621 tatgttttta tgattgtatt agattaatat ttaaattaca ttgaaaagaa aaaaatcatt
     1681 actatagaat aatatcttta tcaaaagaac attcttatgc agaatatggt tatgagtatc
     1741 taagagaaat ctcaaaatat ataggatagc agtatgaatt tttatagatt ttattaatac
     1801 aataaatcaa atgagaaaaa aaatcaaaag aatacaataa tcacttgcaa aaaatatgct
     1861 attgtgatag acttaaatca aaattaggaa ttgtaagaat aaggaaaaag ataagtaatt
     1921 attttaacga gtcaaaattt aagaaattgt gatttatata taagatagag gttgtttgta
     1981 aatttaaatt ggttgaagag gaggaatatt atatgatgcc attgatatta tcaaatacag
     2041 gtgaagaagt agttataaag aaaatttcgg gaagtgataa gacgaggact tttttaaata
     2101 gtataggttt tgtagtaggt acaaatgtta aaatagtttc taagatagat ggtaacttaa
     2161 taatagacgt aaaagatact agagttgcat tagataaaaa aatggcaagt agaataataa
     2221 tatagggagg taatgacatg aacagactga aggatattaa gtgtggagag actgtaaaag
     2281 tcaaaaagtt agaaggagaa ggagctacac gaagacgtat aatggatatg gggattacaa
     2341 gaggtgttaa tatatttata agaaaagtag ctcctcttgg tgatccaatt gaggtaacag
     2401 tcagagatta tgaactatca attcgtaagt cagatgcaga aaaaattatt gtggaataat
     2461 aaagattaat tagagtaggg ttaatgtaat tatataatga gaatgatttt ctgtatcaca
     2521 aacagtgatg tatttaaata ataatggagg gaatggaaat gtcaataaaa ataggtctca
     2581 taggaaatcc taactgtggt aagacaacaa tgttcaatgg gcttacaggc tcgtcgcaat
     2641 atgttggtaa ctggccagga gtaacagttg aaaaaaaagg tggaaaatta aaaggaaata
     2701 aagatgtgga aatagtagat ttaccaggta tttattcttt atctccatat acattagaag
     2761 aagtagttac acgtaatttt atgctagatg ataagccgga tgcagtaata aatattgtag
     2821 acgcatcaaa tatagagaga aatttatatc ttacaactca agttttagag ctaggtatac
     2881 cgaccattat agctcttaat atgatggata ttgtaaataa gaatggtgat aagataaata
     2941 ttaaagaact atctgaagtt ataggttgcc cagtggtaga agtaacagca gttaaaggtc
     3001 aaggaattat ggaggctgct gaaaaggcag ttgaacttgc aagtagtaac aataaattaa
     3061 atttcaaatt accttttgtt gatgaaagta aggacgcaat agaaaaaatt gaaaaaataa
     3121 tagaagaaaa gactccatat atagatgtag aaacacgctg gttagctata aagctatttg
     3181 agagagatga aaatgttata caaaaacttg atatttctaa aacaatattg aatagtattg
     3241 aagaaataac tagaaattgt gaagatgaat tggatgatga tagtgaaagt ataataacag
     3301 ccaatcgtta tgaatttatt agttcaataa tttcaagtat aataaagaag aatcgaaaag
     3361 gtaaggaaac tgtatcagat aagatagata agattgtaac aaatcgtata ctagcattac
     3421 ctatttttgc acttataatg tggggagttt actatatagc agtaagttca ttgggtacta
     3481 tagcaactga ttggacaaat gatgtactgt ttggagaaat tattcaaggc aatgtaagta
     3541 attttctagc ttctttaaat gtagcagagt ggttacaagg acttgtagtt gatggtctaa
     3601 ttggtggagt tggagcagta ctaggatttg taccacaaat catgctttta ttcttattat
     3661 tgtcaatatt agaagattgt gggtatatgt cacgtgtagc ttttataatg gatagaattt
     3721 tccgtaagtt tggtctttca ggaaaatcat ttataccaat gcttataagt tcaggttgtg
     3781 gagttccagg agttatgtca actcgtacta tagagaatga tagagataga aaaatgacta
     3841 ttatgttaac tacattcatt ccatgtggag ctaagattcc aataatagca ttatttgcag
     3901 gggcattatt tggaggtgct tcatgggtag ctccatcaat gtatttctta ggtattgcaa
     3961 tgataatcat ttgtggaatt atattaaaga aaactagttt atttgcagga gaaccatctc
     4021 catttgttat ggaattacct caatatcatg ttccaagtgc aaaaggtgtt ttaatacata
     4081 tgtgggatag aggaaaagca tttataatca aagcaggaac aattatattt gttgcttgtg
     4141 gagtaatctg gttcttacaa tcatttaatt ggtcattaca aatggtagat gctggagata
     4201 gtatattagc tagcttagga aatatagttg caccaatatt tgctcctctt ggatttggaa
     4261 attggcaatc atcagtagca acagttacag gtctagtagc taaggaaaat gtagttggaa
     4321 catttggagt tttatttggt atatcagatg caacagagca agacccaaca cttcttgctt
     4381 cagttgcaag tatgtttaca gtagctagtg cgtttgcatt tatggcattt aatatgcttt
     4441 gtgcaccttg ttttgcagct attggagcca taaaaagaga aatgggttca tggaaatgga
     4501 catggattac attaggattc caaactttaa cagcgtatat aattgctctt cttataaatc
     4561 aagttggaag tttagtttta ggtactggag gaagtattgc tggggcaata atatctatat
     4621 ttatagcagt agcagtagtc tttgtagttt taacttattc aaataaaaat atgaaaaaag
     4681 aaaaaatggg taaattaagt tatatgaaaa attaattcat ataaataagg agtgatgaat
     4741 atggctacat ttattatagc agctattgta gtagtattaa tggcactagc tgtaatgtat
     4801 atggttaaaa attcaaaaaa gaatggtagc agttgtggat gtggttgttc tggatgctca
     4861 agtagtaaga gttgtcatag cgctaagaga taaataggat aagagcctga taaaaagtta
     4921 aattgcttta ttatcaggtt cttatttttt atataaataa caaatttcat atttgaagta
     4981 ggttagacta tgctgtaatt ttactgtata attatatata aatatatgat taggagggat
     5041 tattgctaaa ttaagcaata taagtgaata ttatgtttaa atttgatgtt acaaacgagc
     5101 ttatgtgtat agcaagggga agtggtaaat tttttgcaaa aaaaggtgct atggtagcct
     5161 ttaaaggaaa ttttaatttt gaaaaattgc tgttaggtcc aagtaatggc ggtggattgg
     5221 gaagagcatt attaggtcat gttcagaggt cattgacagg agaacaaatg ccaataatgg
     5281 tggttgaagg tgaaggtgaa atttacctag ctcaaaatgc ttaccatgta gatgtaatat
     5341 caattgaccc aggtgattcc atttatgttg agagtgaaaa cttattggca ttttctgaac
     5401 aattaaatta cagtgtaaaa ttgattggtt caggggtaat atctcaaaaa ggtcttttta
     5461 ctacacattt agtaaataaa acaggtcaaa atcaagatgt tgctataata acagatggta
     5521 atcctctaat tcttgaagga ccttgttgtg ttgacccaga tgcattagta gcttggactg
     5581 gaagagaacc atatcctaaa gtatcaaaac tatcatggaa gacttttatt ggtcagactt
     5641 caggagaatc ttatcatatg gaatttgttg aacctggaca aatagtcatt atacaaccat
     5701 ctgaaagatt gtctggctta aaaacagatt ctacaccttc tgatagaatg agttcaggtg
     5761 gattaagact tggcatagat taaatcttat gatatagctt aattattatt taagatatta
     5821 aaaaataaaa agggtagcag ggatagatat gttaaaagga gttttatatg tttgagaaaa
     5881 ttcataaaag gtttaaagga tattatattt ttttaataat tttaatttta tggaagatag
     5941 tatgtgtttt gggtatatgg agtagctata ttctgcctcc tcctgaaata gtcttagata
     6001 cttttataaa aatgatatat gacggttcta ttttcttaaa tgtttatgcc agtgtaagaa
     6061 ggatattaat aggtttttta ataagttcat tgatggctat accacttgga gttgtatttg
     6121 gagtaaataa aaaaatgtat gaatatttca aaccactatt tgaatttatg cgaagtacac
     6181 ctccactagc tttggtgcca atgcttattt tatggtttgg tataggtgaa gaatctaaaa
     6241 ttataattat tattttggca tccttttttc caatattttt aaatacattg aaaggtataa
     6301 aaaattgtga caataaatta atagaagtag gtaaatcttt taatttatct aaaaagagaa
     6361 ttttttataa aataataatc cctaattcta tgctggatat tgttgttgga cttagtttag
     6421 ggcttgggta tagttttagg gctataattg gagcagagct tattgcagct tcttcaggac
     6481 taggatattt gatttctgat ggaaaagata tgtctagaac tgatgttgta ttggtaggaa
     6541 tttttgttat tggattttta ggtatacttt cagattatct attttcaaag ataataacaa
     6601 aatttagcaa agggaagcag gttgatttaa atggttagaa gtggattttt gctagagaat
     6661 atatataaga aatatttagt tgataataaa gagcatttag ttcttgataa tatatcttta
     6721 aatatatcta gtgaagagat aactgttata ttaggtgaaa gtggatgtgg gaaaacaacg
     6781 ctacttagaa ttttagcagg gcttgaaaat gctactagtg gaaacatata tttttttaat
     6841 aatgataaaa agtgtactcc aaaagtagga atggtatttc aagaaagtag acttatgcca
     6901 tggctgaatg taagtgaaaa tatactttta catactgaaa aagataatag aaataaagtt
     6961 gatttagaca agtatttaaa aatgatgaag cttgaaaagt ttaaaaactc atatccaaat
     7021 gaattatcag gaggaatggc tcacagagta agtatagcaa gagcactttc cttcaatccc
     7081 gatattttgc tgatggacga accatttgca gcgctagatt acttcacaag aagaaaaatg
     7141 caaaaagaag ttgtaaatat ccataaaaat actaaaaaag gtgtagtatt tgtaactcac
     7201 aatattgaag aagctatgga aatagcaaag aaaataattg tttttagtaa aaataaaagg
     7261 attaaacaat ttagtgtcga agatgaatat aatagagatt taactaaaaa ctattatata
     7321 aacttgaaaa aagaaatatt aagggaattg ggggagtttt aattgattaa aagaaacaaa
     7381 aagattttag cagtaatgac atctttaata atggtgttgg ttgggactgt aggatgttca
     7441 acaacaaatg ataagaaaga tgaacctgca aaaaaagagg ctagtttacc taaggaaata
     7501 aatttaactt atgtaaaatc accacttaat gttcctagta tattagaaaa aaaggatgat
     7561 ttatttggaa aggaatttaa aaaggacaat ataaaagtca acttccatga attgacaaca
     7621 ggaccagagc aaactcaagc attagcagca ggtgagctag actttttaca tgcattagga
     7681 ggaacgtctg caataatagc tgcatcaaat ggtgttggtc ttaaaattac gaatatttat
     7741 agccgttcac caaaaggata tatgcttata actaataatg ataatattaa atctataaaa
     7801 gacttaaaag gtaaaaaaat tgtaggtcca aaagggacta tacttcatca actgttgatt
     7861 gctgcacttg ctaaagaagg ctatactata gatgatgttg aatatgtaaa tatggatatt
     7921 cctagtgctg cttctgcatt agaaagtaaa agtgctgatg tagcaatgct tgcaggtcca
     7981 gtggcattga aaacgataaa ttcaggtgcc aaaatgattg taaatggaga aggattagta
     8041 agtggaataa tagttactgc tgtaagtgat gactttgctg aaaaatatcc tgaattagta
     8101 aaaagattta tgaaggttca tgaagaaact ttaaaatata tgaatgaaaa caaggatgaa
     8161 gtgatggatg ttgtatctaa agaagttggt ttatctttag atgaaacaaa agaaatgtat
     8221 tcttggtatg actttagttc taaaataacg gacaaggata taaaagaatt agaagataca
     8281 caggaatttt tgatgtctaa tggaatgcaa caaaagaaaa taaatattaa agatatgttg
     8341 tacaatcaaa attagaatac tttaaaatat taaagtggaa aatacaccct tatttattat
     8401 ggaaactgta tggtaaaaga gggtgtattt ttattattat acaaaaatac aattaaaaac
     8461 aacttactat tttatctaca cttatgtagt ctagtaattt tctcattttc ttacggtgaa
     8521 gtaaaaactt tgaatatgtg tcctcggatt gttttgtgtc accataaact ttccaatatt
     8581 taatcataga aacttcataa ttactatatc ccataaatcc aatcacatct ttaagaggat
     8641 atcctaaaaa aatacctatt tcatcaggaa aaacatcact cttaagttta tttactaaat
     8701 gctctaaata agcattaaca ttcgtatttt gtttgtatcc taaaaacttt aaaaagttta
     8761 ctgttttttt acattttaat ttttctgata atgctttttt atttacaaat aagattttta
     8821 atcccttttt ttctttatca ataactataa aatctatttt cttacattta tatagatatt
     8881 tataaatatc attaattttt tcctctctat ttacatcagt aaacgaaata tttattattt
     8941 cagcaggttt agagcctaaa aagactggac ctaaaacctc taaaagccat tttatatatg
     9001 atgattcaat tttattttta caacaaaatt gtgaattaca acaagacata aaagataacc
     9061 tccttcaaat gaaaattaat atcaagtaat tagattatac tatgaatgat aatcaatatc
     9121 aataataaaa ttataatttt tttatttaat tatttattca ttatgatttc caaaaaaata
     9181 aattataact tgtttaattt tagtaaataa aaaataaaaa tatagaaata ctaaattaag
     9241 aaagaaaatt attaaaattt tttgtagtac aaaacatcta ggagggtaat aaaaatgaaa
     9301 ttaaaaagaa ctttatttgt aggattagca ttagtattag ctataggaat aactgcatgt
     9361 gctagggata atgaaaaacc aaatgaaaat gcaggaaaaa atcctaatga atctgtaaat
     9421 aataatgaag gatatatgaa ttattattca acaagttata atgattatat agcaggtcta
     9481 aatagatatt ctatttatga tactccagaa agtataaaca atcagtttaa agataaagaa
     9541 tatccaggaa atgaaaaata tctaagtgat gtaaaggctg catataaaga tagtagagat
     9601 aaaatacaat catttgtaac atctcttaaa aaagacggta aaacagaaga tacagagtta
     9661 aagagaatga atgatgacct aatagctgaa ggggaaagat tagtaaaaga tatagatgag
     9721 agaatagaaa aattagataa ggtatctgat gaagatatga aaaaaggtca aaatgatttt
     9781 atgaaactag ttcatggaac tgaagatgtt ggaaacgata ttggtagtgg ttttagaaaa
     9841 atgataaaag atatgaatga tagattggga ataacaggaa ataataaaaa ataatagagt
     9901 aaatagtttt aaagttagaa ataaatattt gaaatatagc gaagccttgt aattaatata
     9961 ttttttggct tcgctttttt gtatgttatc aagatactaa tttttatctt gtaacctcat
    10021 attttcatta tagtttattt agaggttatt tttttatgtt tttacttgtt ttttctcttt
    10081 ttaaattttt gttttgcaaa attgtaagat tcttctatta aagttttaaa gttctcaaat
    10141 gtcttttcag tagggcttag gacgcatatc catcccatcc atgagtagat aggatgtggc
    10201 atgattgtgt ccaattttgt aaaatcaaaa tccatatcaa ctatttgccc tacacctgga
    10261 cgctttggaa tatatccaaa catttcaata aaagtttctt tttttaatcc tatattcaca
    10321 cggtatacat ttggacgact aaccaacgaa cctttatcat gattactgtc tttttcttta
    10381 atggtaagaa catatacccc tcgctttaaa acaccattag gattgtagta aattgctcgt
    10441 tccccccaac tatccattag tacaacatca tctaagtttg atagacaata attgagaata
    10501 tcatcagcct taatagtatc attttccata aaatatcaat cccttctttc tttaatatta
    10561 ttattgcttt agatgcaaga atatcttatc atataatggt gacaattatt gtcactatat
    10621 ttaaattagg agtatgaata tgtcaagagc tgagcgttta attgaactga tgataacaat
    10681 aaatgcaaaa agaagtttta cagcgggaga gctagcagaa gaattttctg tatcaaaacg
    10741 tactatacta agagatttac aggtattgga aagtgttgga tttccactat attcaaaagt
    10801 tggagcggct ggaggatatc atgtacttaa agaacgtata cttccaccaa ttacattttc
    10861 agagagtgag gtaaaatcta tattctttgc atatcagtca ctagagtatt ataatgattt
    10921 accatttgag caagaaacta tttctgtttt aaagaaattt ttaaattgta taccaaatga
    10981 tattcaatat aatattgaga atatgagacg taaacttgtt ttttggacac cagataggca
    11041 ttgctcaaca ccattattga aggaattgtt taatatagta atgaatgaat ttacaattaa
    11101 aattgaatat tcatcaaagc aaaaaaatag tatacgtacc attgttccta ttggtttgta
    11161 tgctatgaat ggactttggt attgtccagc ttactgtata aaatcagaat ctgttaagga
    11221 atttcgtgca gacagaattg tgaagatttt gagtatagaa aacttatcaa ataagaaata
    11281 taaggtttta tcttctatac atgattatct taagaatatg gaagtaggta cagattatca
    11341 tattaaaatt aatctgacag atgagggagt aaaacgttgt gaaacagaat ttttgttggc
    11401 aagaggattg aaaattttgt ctaagggtgg atacatagat atgtatatac caaaatctac
    11461 acttaattgg gtggctgaat attttttaac ttttggaaaa aatgctacaa taatagaacc
    11521 tatagaactt aaacatttga ttaaaaaata gctacgcaca cttgtgactt tagtcatgag
    11581 ttagtagcta aattagtcag catataggga aacttatatg tagagatagg ataa
//
LOCUS       DAMJVX010000111        11410 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMJVX010000111 DAMJVX010000000
VERSION     DAMJVX010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11410
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.115"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            179..2077
                     /locus_tag="PC407_002580"
     CDS             179..2077
                     /locus_tag="PC407_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HDF2498015.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            complement(2205..3326)
                     /locus_tag="PC407_002581"
     CDS             complement(2205..3326)
                     /locus_tag="PC407_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HDF2498016.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLEEFKTVIHPEKIADYISELIDNHYS"
     gene            3525..3857
                     /locus_tag="PC407_002582"
     CDS             3525..3857
                     /locus_tag="PC407_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2498017.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            3844..4902
                     /locus_tag="PC407_002583"
     CDS             3844..4902
                     /locus_tag="PC407_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDF2498018.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNIKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSVTIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            4895..5965
                     /locus_tag="PC407_002584"
     CDS             4895..5965
                     /locus_tag="PC407_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDF2498019.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDISVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSTSSYLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSNMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            6067..6603
                     /locus_tag="PC407_002585"
     CDS             6067..6603
                     /locus_tag="PC407_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2498020.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            6720..7427
                     /locus_tag="PC407_002586"
     CDS             6720..7427
                     /locus_tag="PC407_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2498021.1"
                     /translation="MNNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRKLIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVIGYQGEVSKEQN
                     LEKLFMKVVAENRKRGE"
     gene            7429..8145
                     /locus_tag="PC407_002587"
     CDS             7429..8145
                     /locus_tag="PC407_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2498022.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWAGKMIYCIICCSIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLTNMFFT
                     IVGVAVFSTTEMWYVFPYSIPSRLMVVVLHILPNGLPVPEGSELLNNAVILPGVLISV
                     ILFVLFALVTRAWFNNREVK"
     gene            8147..8905
                     /locus_tag="PC407_002588"
     CDS             8147..8905
                     /locus_tag="PC407_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2498023.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            9049..10437
                     /locus_tag="PC407_002589"
     CDS             9049..10437
                     /locus_tag="PC407_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HDF2498024.1"
                     /translation="MLRSKKSNNKKVEKLRTIFIKYLSLFFVMTISFALLLMLSFSVL
                     LSSGVILPANYSEKQFDKYKEKIISSEKAIEDIIPNLYEYGVYTLDGNLISGTFNKKE
                     SKQVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKLVSEYSSPVLRAYLPKPEMLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNILF
                     SMDKMKLALKNSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAINMSEFIKDVLLQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVSNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(10513..11334)
                     /locus_tag="PC407_002590"
     CDS             complement(10513..11334)
                     /locus_tag="PC407_002590"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HDF2498025.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
BASE COUNT         4468 a         1245 c         1867 g         3830 t
ORIGIN      
        1 atattttttc caataaaaaa tattgcgaac tttttatact tgatatataa taatataaat
       61 aaaaacacga ataaaacacg aataatttgc aagaaaaatt catgaatgta aggaaaatga
      121 aatttatctt gtatttaaca aaataaaata tacataaaaa ttatgggaga gtggaaaaat
      181 ggatagtcta ttatacgtaa tagagaaaga taaacacact aatgaagaat taagagaaat
      241 cctaaaaagc aataaaaata taagatttgt ttccttaatg ggggttgatt taggaggaaa
      301 tgcaacagat gaaaagattc ctgtagaatt atttttggag gatatagata aatttttgga
      361 atcagctata cagactgatg gttcaagtgt tgagctttat aatatagcta cattaaataa
      421 tgcgaaagta gatttaatgc cagataaaag ttgtcattgg tatgtagatt acaatatgga
      481 atatatagac gaagaagtag gtctacctgt tggaacatta aagatacctg catttttaat
      541 acatgataat aaaaaagtat gttctagagg agtacttcaa aaagcagata agtactttaa
      601 aaagtcaatg tatgaaatat ttagagaata tccacatgta ataaataaca taggaataga
      661 ttcagtagat gatatagagg aaataatgtt aacagcagct acagaacttg agttctgggt
      721 taatactcct gaggataaag ctgacttaga gaaattatat gtatcacaaa gcctaaaaga
      781 gcaatactgg aaaagaactc atggtattat aagaacttgt ttagagaaaa gtttaattat
      841 acttcaaaag ctaggtgtaa accctgaaat ggctcacaaa gaggtcggag gaatacaaag
      901 ttctattagt atagatggaa gaacaaacca tgctatggag caattagagg tttcttggaa
      961 gttttctaca ccattacaag cagcagacaa tgaattatta gtaagagatg ttatagagga
     1021 tgtatttaca tcacatggac tagaagttac atttaaagca aagccaattc atggagtagc
     1081 aggaagtggt ggacatacac atgtaggagt aagtgcgaag ttaaaggatg gaagtataaa
     1141 aaatctattt gctccaaaag atttaaaaga agattatcta agtgaactag gatatggggc
     1201 tcttatggga cttctttaca attatgaagt attaaaccca attgtaacag cttctaatga
     1261 tggatttaat agattagttc ctggatttga agctcctgta tgcatagtta catctttagg
     1321 gcatagttat gagattccat caagaaatag gtcagtgctt gttggattaa taagagatat
     1381 gaaaaatcca aagactgtaa gatttgagtt aaggtcacca agccctctat ctaacacgta
     1441 tttagtaata gcaggatgtt atcaaacaat gttggatggt ataaaagcag cagctaagag
     1501 tggtcttagt acaaaagaac ttgaaaaaga gttatctaaa aatgtaggtg aagaaagttt
     1561 ctatttagaa aaagatagag catatagaga tgaaaatgat gtatttgaac attatagttt
     1621 ggaagaaaga aatgcaagat ttggaatacc accggctaca gtttatgaaa atatgaaaaa
     1681 cttagaaata tatgcttcaa aattaaaatc tttaaaacaa ggtgatgtat ttactgatag
     1741 tataatagaa tcatttaaga ttggagctat agataagtgg caaaaaaaat taaaaactag
     1801 aataatagag gctgggattc aaaaaataag aagtattgtc aagatacaca caaaagaaaa
     1861 tatggatgcg cttgatgagg tagtttggaa ctcaatatcg gatttaaagt tcaatattat
     1921 gaaggatact ttaactagag aatcactatt cactagagtg agagaagcta tagaaaataa
     1981 ggattaccag actgcttcag atttacaaat agaattaaag agaagtatgg aagaaataca
     2041 acaactgtat atgcagtaca agaaaaatat atactaatat aacaatttga ttttgatatg
     2101 ataaaggcaa tcaagtatta ttaaacacta tgagtaaatt ctcaagtgta aaagcatact
     2161 tgattgcctt taattttatg tttgaaattt tttaatagta caatttaact gtaatgatta
     2221 tctattaatt ctgatatata atcagcaatt ttctctggat gaataactgt tttaaattct
     2281 tctaaatttt ttttcataaa gtttattata gaatcaaatt catttgatac taatttataa
     2341 aatatgtttt taaggtctag gtcatcacta taaatcatgc caagaccctt atctattata
     2401 aatttagcgt tttcaatttc ttgtccaact tttggcattt tcacgagtac tggagtttgt
     2461 gattgaatag cttcaaatat agttgctcct ccaggcttag ttagcattag gtcacattct
     2521 cttattagat tatacatgtc gtttacaaat ccaagtactt taatattttt tagaggtttt
     2581 ttatgagtta gattgtcata taattttttg tttttaccag taactattgt aatttctata
     2641 gaattactat gttcttcaat aaattcatct atccaatgca taaaatcctc atctatatca
     2701 aataggcctc ttcctccacc tagaagtaat agtctatatt tatcacaaca tagttcttta
     2761 ctttcaattt gaaatctctt atcaacagga actccagtaa cctttatgga gtctggattt
     2821 attcctttct gaaaaaatct attttttatt tcaggagaag gaacaaaata caaatctgta
     2881 ttttcaaaaa cccattccat actatctaca acatctgtta taactgtaag tgtaggtata
     2941 tttatatcag gattttttat cttaaagtta tttacacaag cagccgctag tggaaatgta
     3001 gagataatca aatctggatt tatatcagca atatactcag aaagtttagg tgtatataat
     3061 ttatgagata aatcatgtct tgaatcaaac gatttcttaa agtaataata gtaattatat
     3121 aatccaggag tatattttgt attgttttca taaagtttta ccataggttt attcatcatg
     3181 ggaatacttg catttataaa gttttgtata acaacattat agttactata tttttcaata
     3241 atacattctt tgactgcttt tgcagcactt atatgtccag caccaaattg agcagttaag
     3301 attaatattg ttttatcatc tttcatttta gccaatttat caccactttc ataaaatata
     3361 tctgattaga gtattataca tttatagaag gatttaatca acaagaatat ttaaaaataa
     3421 ctattgtgca gaacatagta ctgtgttatt atgaggatat aagaagtttt aacatagtac
     3481 cttgtaaaac acaatactgt gtatttaaag aaaggatgga gtatatgaac acacaattta
     3541 gaaagggtgt tttagaaatt tgtgtacttg ctctcatatc taagaaagat atgtatggat
     3601 atgagatagt acataacatc tctaaagtaa tagatgtaaa tgaaggaaca atatatccta
     3661 ttctaagaag acttacaaaa gatttgtatt tcgaaacata tatactagaa tctaatgaag
     3721 gtcctgctag aaaatactat agaataactt cactaggaaa agagaattta tccaatctga
     3781 tggaagagtg gagagaattt gtaaaggctg ttgagatttt aataagtgaa gatgagggag
     3841 gtaatgaact tgaataaaac tgagtttctt gaaattttaa aggattatct aaaaaaagat
     3901 ttttctgaga gcgaagttaa tgatatattg agagattatg aagagtattt cgtagatggt
     3961 cttatagagg gaaagagtga tatggagata atagcatcac ttggttcacc aaaatctata
     4021 gccaaagaac ttatcttgca gactaaagaa aaaaatattg aagaggaaaa aataataaat
     4081 acagatgttt tgaatgataa gtttagtaaa tttaaatcag atatgaaaaa aggttactct
     4141 atcttaaaag atgaagcaaa agaaaagtat agtaaatcaa aagaaaatat agatggaaaa
     4201 ataatgaata cagatatatt aaatggtaaa tttagtaggt ttaaatcaaa tataaaaaag
     4261 gattactcta tcttgaaaga tgaagctaag gaaaagtaca ataaatcaaa agaaaatata
     4321 aatgataaac ttacaccaag tatagaagaa ggtgtagggg gattatctag acaagttata
     4381 aagggattat tagtagtgtt atctttattt ctaattgttc cagcatttgc gtttgtatgt
     4441 gctattgtat ctgttactat tgcattagta tgttcattaa ttgcttttgt tatattaatt
     4501 ccatttgtga ttaaatttat agcaatggtt ccagatgtta tgatgttttg tattttcgca
     4561 accatagcat ttgtagggct tgagatactt gcatggcaaa ttttattatt tgctataaaa
     4621 ctaggtaaac aattactaaa taattatata cattggataa agagaaaaaa tatttatata
     4681 aaggcaaatg aaaagatgga aaaaagaaaa aaagctactt taaatgatga agatttgaat
     4741 aaagacatta atgaagattc atataataat gattactaca atagagaaga agaaagcata
     4801 aaagaaactg atgaatataa tgatgatagt ataaattttg aggcgatata taaggacctt
     4861 gaagatatag aaaataagaa aggagaagat aaacatgaat aaaaaacttg ctatatttgc
     4921 tgcagtttta ttggtaatag gtataatagg gacaacatgg tcagggattt tagttatgcc
     4981 aagcctaata aactttggtt tagaaaagga agctgaattt aaaaaagaaa ataaacttta
     5041 tcaagaaaaa gtaaatattg acaagttaga tatatctgtt gacaatataa acgttacaat
     5101 aaagaaaagt tcaagtgaag atgtgcgtgt aacaacaaga ggaaataatg agttttacaa
     5161 atacaatgta actttaaaag ataagacttt agttgtaaaa ggtgaaagaa aatatgaaaa
     5221 taaaattaaa aagattaaaa atttcgacca attattaaat acatctataa atagtatgtt
     5281 ttcacatgat tatagagaaa ttataatata tgtaccaaat aatgtagaca taaatgcatc
     5341 aagtacaagt tcatacttat ttgtatatga taatgtagct tcaaatacta taacatataa
     5401 aacttcttat ggaggtttta gtagagctat aacagagaac aaagtaaata gattagaaaa
     5461 cctaaactta atatctaata gcaatcttca cttgtcaaca aaatcaattt taggcgttaa
     5521 aaatgtaaac atagaatcag agtcattgga tatatcttca gagaatgaag atgtatttat
     5581 caataatata gaagagtata tacctgaaaa tgtaaatata aaagagaaaa taggtagaaa
     5641 ttcaaattat gaaagtgaat tttacttgta ctctaatatg ccaattgcta agttcctgga
     5701 catagaagtt ccaaattcta aggtaagact tgatatacca gtaaataagt ataaatttaa
     5761 ttgtgatata aagtcaaaag aagcaattga agaatttaat aatgatgaag aatatgacaa
     5821 tgattcatat gattatgaac atagtgaaga acatagcaac aagtatagaa atacgaggga
     5881 aataaaaggg cttttgaata aaaatctgtc gaatttagag aaagaatata ctataaaaat
     5941 aaattcaaat agcttagaat tgtaaataat ttactataat aatttataaa ttgatttaaa
     6001 agtataaaaa tataatctat actatggtat aataagtatc gaaagagaat taggagaggg
     6061 atacttatga ataaaatagc agtaagtttt ttaataattg ctactacttt attgagtaca
     6121 gcatgtatgg attatagcat cagtgcagtt gagctagttg attctaaaga atcagcagtt
     6181 gtaaagaagg atgaagatgc aaaagaagaa acaactagta aaatgattaa ttctaaaaaa
     6241 actactaaaa tacctataga gataatttca aaggatgaga aaatagtaaa gtacttacag
     6301 attgatgaag aaagttcact taaggataaa ctgcgattaa tcttagatac attatcaaat
     6361 gagtatttta atggtttaga catggaagta gaagttaagg aaaaagataa tcttgttaaa
     6421 ataaacttga tagaaccaga taaaaaaagt agagtttctt ggaaagatga ttatttgaat
     6481 gaacaaaata taatatatac tataaataat attattaaaa atgtcatcca agaagaagat
     6541 aatagcattt ggattgaaga agtagagata tactacaatg gaaaattaat agaattaaga
     6601 taaaaaaaga tgtgttttta acacatcttt tttttgtttt ttaattctta taaaatcctt
     6661 atatttatga tttagaatga ttcttgtaaa taagtttgac aaaagaaagg atgaaaatta
     6721 tgaataatta tattttgcag gtaaaaggag tttctaaaaa atttaaggag cagatggcag
     6781 ttgataatat ttcactagca attaaatcta attctatata tggattatta ggaccaaatg
     6841 gagctgggaa atctacttta ttaaaaatga taactgggat tttaaatcca acttcaggaa
     6901 aaataatgtt tgagggacat agatggacta gaaaggattt atcaaatatt ggttctctta
     6961 ttgagtctcc tgctatatat gagaatttaa cagcacgaga aaatttaaaa gtccatacta
     7021 ctttacttgg tcttcctgac tctcgaattg aagaagtttt agagactgtc gacttaagag
     7081 atacttttaa aaagcgttca ggtcaattct ctctaggtat gaagcaacgt cttggtattg
     7141 ctattgcatt attaaatcac ccaaaacttt taatcttaga cgagcctaca aatggtcttg
     7201 accctattgg tattgaagaa cttagaaaat tgattcgttc ttttccaaaa caaggaatta
     7261 cagttatttt atcaagccat atcttgtctg aagttgaaca agtcgtagat gaaatcggaa
     7321 taatttctaa tggagtaatt ggttatcaag gagaagtatc aaaagaacaa aacttagaaa
     7381 aactttttat gaaagttgta gctgaaaata gaaagagagg ggagtaatat ggaaagattt
     7441 attagagtgg aatttcaaaa gatgaaagga acttttggaa tgaagttgat atggatagtt
     7501 ccagtgtttt gtatactttt gtgtggacta ggtggagggc aaaatgctgc tttcaactgg
     7561 tggtatacga catttcttcc aggagcatta acattaatat cttctttaat agtattaaaa
     7621 gatgaaaaaa gtaaatatcg tggtatttta ccattgccaa ttgaaatgaa agacgtttgg
     7681 gctggaaaaa tgatttactg tattatttgt tgttctatag catcgattat ttctgcaata
     7741 ggtgtagttt ttgttgggat aatctataaa gaaactattt caataggtca aaatatcatt
     7801 gcaactatat taattatttt actatttatg tggcagattc ctctttgtat gtttttagct
     7861 tcaaaatttg gtatatttgt tacagttcta acaaatatgt tctttactat tgttggagtt
     7921 gctgttttct caactacaga aatgtggtat gtatttccat attcgattcc ttcaagatta
     7981 atggttgttg tattgcatat tctaccaaat gggcttcctg taccagaggg gagtgaactt
     8041 ttaaataatg ctgttatttt accaggagta ttaatatctg ttattttatt tgtattattt
     8101 gctttagtta caagagcttg gttcaataat agagaggtga agtaatatga gacaactaat
     8161 aagaatattt tgttctgatt taataaaatt aaagagaaat tttataatac taatgcacat
     8221 ttgcattgct ttaatgggga tgggattgtt tttatggtat tataaggttt caggagctga
     8281 taatatatta aagatagtag catatttaca agttatagca atagcttttc ctttattgtc
     8341 aggtgttgtg tgttcaatgt ttgttgaaca agaatattat gcagggaatt ataaacatat
     8401 gttaacaaca tctaatccaa aatatcttac tcttataagt aaatatataa tcttagtatg
     8461 tcttggtttt gtagcaacat tagtgtctgt attaggtttt aaatttggta tatctaaggc
     8521 ttactttaca tcaagctttt atatgatttc aattctaatt ttaattgggt gtaatttatt
     8581 tgaatacata ttacatttat tcttaagttt aaggttcgga aaaggaacat caattggagt
     8641 aggtatagtt gaaatgttac tttcagcatt attattaact ggattgggtt ctggcatttg
     8701 gcagtatttt ccttgtgcat ggggtgttag atttataact atttggtcgt ctttttcaag
     8761 ttctaaaacg gttgaatata taaaggttga atcaataaaa gcatatcaat caacaggttt
     8821 aatgtgtggt tttgtaacaa ttttagcatt tgtaatttta tgtatttggt tttctaaatg
     8881 ggaaggtaaa aagtcagaag aataaataaa aattgactgt ttatttacat tatatttata
     8941 ttattatgtt aaaatataac ttagcaatga aaaatataaa ataatcaaat aaaaactggt
     9001 taggtattaa ttatgaatta attaaaatat ttggaggata tggcacaaat gcttagaagt
     9061 aaaaaatcta acaacaaaaa agttgaaaag ttaagaacta tttttataaa atatttatct
     9121 ttattctttg ttatgacaat ttcatttgca cttcttttaa tgttatcatt ttctgtatta
     9181 ttatcatcag gtgtaatatt accagctaat tattcagaaa aacaatttga taaatataaa
     9241 gaaaagatta tttcaagtga aaaagcaatt gaagatataa ttccaaattt atacgagtat
     9301 ggtgtttata ctttagatgg gaatttgatc tcaggcactt ttaataaaaa agaatcaaaa
     9361 caagtatgga atttaatgag agatattgaa gaaaggcatg catattcaga aagttatata
     9421 aagtttttta agaaagatga agtttttata ataaaataca aactagtatc agagtatagt
     9481 tctccagttt taagagcata tttaccaaaa ccagagatgt taggtatgat tatttttagt
     9541 ataatatttt ttattgaaat tgttatatta tcaaaagtat ttggcaagaa atttaatatt
     9601 gaaatggagc ttttaaaaaa tactacagaa aaaatagaac aacaggattt agattttgtt
     9661 gtagaatcta gtaaaattta tgaaattaac aatatccttt tttctatgga taaaatgaag
     9721 ttggcgttaa agaattcttt agaaaaacaa tggatgctag aagaaaatag aaaggaacag
     9781 atatctgcac tggcacatga tataaaaaca ccattaacaa ttatacatgg aaatactgac
     9841 ttattaattg aaataaatga aaatcctgag ttattagaat acatggaata tatctcaaag
     9901 ggtgtcactc aaatagaaaa atatattaat actttaatag atatatcaaa aacagagaat
     9961 ggctatatat taaataaaga agcaattaat atgagtgagt ttataaaaga tgtcttactg
    10021 caaatagaag cattagctag tactaaaaac ttagatgtgg agttttataa acaagataat
    10081 ttgcctaaaa atataactat agatagagaa ctgttattca gagctattat gaatataatt
    10141 tcaaatgcta tagattattc accaagccag agtaaactat acataagtgt atcagtaagt
    10201 aataaatact taaaatttgt agttacagat tgtggaagtg gtttttctaa agcagatttg
    10261 tcaaaagcta cagaacagtt ttatacagga gatttgagta gaaattctaa atctcattac
    10321 ggaatgggac tttatattgt aaataatatt gtacaaaaac ataatggaat tttatatatt
    10381 gaaaattctg tgaaaactgg tggtgctatg gtaacaattg aaataccaat agtttagtaa
    10441 ataatatcca ttttgtaaat acctaaattt tatgatttat aggtatttat aaaatggata
    10501 gtttttttga ttttatatat tagttaaatc tgataaaaag ttttcaaaca taactccatc
    10561 atttctatca gtatcaaatt gagatgtgta atttatagtt ttatatataa aatctgttgc
    10621 agtattaacc gctactccca aatcatgttt atttactaac ataccacaaa gtatagatgt
    10681 gaatatatct ccagttccac tgtaagaaca attattgtat ttgactgatg taaagaatac
    10741 atggtcatta tctctatcat aagccaaatt taatatatta tcatcttcta atataccagt
    10801 aataacgact ttgctaggtc ccaaatcaga tacagatttt gcaatatata taagctcatc
    10861 tctattataa tcgcttttag tataatcatt tccagttaag aaacaagctt ctgttaaatt
    10921 aggagtagtt aaatctgaat gtttaactaa ttctttaatt tcctgtctca tttcttctgt
    10981 gaatattgga tacataactc cattatcacc catcacaggg tcaactacga taaaagcatt
    11041 gggataatca tttataaaat tagccactat ctcaatttga tgctttgaac ccaaaaaacc
    11101 actatatata ctatcaaaat tgacttttaa atttttccaa acatttgaat attttaccat
    11161 ttcatcagta aagtctaaga aagtaaattc aggataacca gtttgacttg aaagtatggc
    11221 cgttggaaat ggacaacatt gaacctttaa tgctgaaagt ataggtatag caaccgataa
    11281 tgagcatttt cctattccag ataagtcatt aattgctgcg actttttttt gcataaaaaa
    11341 ccctccatta gtatgtatta attataatga taccaaaata tttatataca tatatattac
    11401 tataaaaaaa
//
LOCUS       DAMJVX010000112        11407 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMJVX010000112 DAMJVX010000000
VERSION     DAMJVX010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.116"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            52..936
                     /locus_tag="PC407_002591"
     CDS             52..936
                     /locus_tag="PC407_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2498026.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            1035..2210
                     /locus_tag="PC407_002592"
     CDS             1035..2210
                     /locus_tag="PC407_002592"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HDF2498027.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEELLKDIPTANKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            2338..3192
                     /locus_tag="PC407_002593"
     CDS             2338..3192
                     /locus_tag="PC407_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498028.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSNESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKIKDDSSC
                     LYLDIYND"
     gene            3236..4471
                     /locus_tag="PC407_002594"
     CDS             3236..4471
                     /locus_tag="PC407_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDF2498029.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREIVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKSISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYISLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNEKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            4572..5513
                     /locus_tag="PC407_002595"
     CDS             4572..5513
                     /locus_tag="PC407_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HDF2498030.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(5742..6095)
                     /locus_tag="PC407_002596"
     CDS             complement(5742..6095)
                     /locus_tag="PC407_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2498031.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMIW
                     LEKLKEDNTYEIYSI"
     gene            6391..7302
                     /locus_tag="PC407_002597"
     CDS             6391..7302
                     /locus_tag="PC407_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HDF2498032.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            7562..7996
                     /locus_tag="PC407_002598"
     CDS             7562..7996
                     /locus_tag="PC407_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498033.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            8019..9554
                     /locus_tag="PC407_002599"
     CDS             8019..9554
                     /locus_tag="PC407_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498034.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVIYK
                     IDCDVILVNLFELIINNCIFSTILGNEVINLEISKYEYEFLEKQFKNMYNNITEDKVI
                     GSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKTEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            9638..11005
                     /locus_tag="PC407_002600"
     CDS             9638..11005
                     /locus_tag="PC407_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2498035.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKARDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMNTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            11040..11264
                     /locus_tag="PC407_002601"
     CDS             11040..11264
                     /locus_tag="PC407_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDF2498036.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
BASE COUNT         4545 a         1049 c         2107 g         3706 t
ORIGIN      
        1 actaataaaa attaaataat attaatcaac ttattgaatg gaggaataaa aatgaaattt
       61 gtgacttttt gtagtagcaa tgaagaaaaa ataggtgtat ttaattcaga aacaaatagt
      121 atatatgaaa ttaatagttt aggattaagt aaattatata ctgatatgaa tgattttatt
      181 gaaaacgtat ctacaggaga tttagaaaaa ataaaaaaca attcatttga aaacgcaaag
      241 tgttataagt tagaagaagt taaattatgt tccccaattg taagacctaa aaaagatatt
      301 atatgtttag gtctaaacta taaagaccat gtcaatgaaa tcccagatgg agttataaaa
      361 aacgtagtta tgccagatta tccaatttat ttttctaaga gagcagacaa aataattgga
      421 gtggatgata agataagctt acatggagat ttggtagaga agttggacta tgaatcagaa
      481 cttgcagtaa tcataggtaa agaaggtata aatatatcaa aagaagatgc ttatgaatat
      541 atatttggat atacaatagt aaatgatata agtgaaagag cacttcaaga taaacatgtt
      601 caatggttta gaggaaaaag cttagacaca catacatcta tgggaccttg tatagtacat
      661 aaagaagaat ttgaacatcc tttaaaatta gatataagta gtgtagtaaa tggagaagta
      721 agacaaaatt ctaatacaga gtactttata tttgatatac ctactataat aaatgattta
      781 tcaagaggaa tgactctaaa gccaggagat ataatttcaa ctggaactcc agcaggggtt
      841 gctatgggga tgaatcctca agtatattta aagcatggcg atatagttga atgtaaagtc
      901 gaaggaatag gagttttaaa aaatatagtt gattaatgga taaatatgcc atataatttt
      961 attagtacaa ataatttaat taatatagga ttgatcaaac ttatgattaa tttattatag
     1021 agagggatga gtatatggca agagaactag aattaaagta tggctgtaat ccaaatcaaa
     1081 aaccatcaaa gatttatatg aagaatggag aacttcctat agaagtatta aatggaaaac
     1141 ctggatatat aaatttttta gatgcattta atagttggca acttgtgaag gaattaaaag
     1201 aagctacagg tcttccagca gcaacttcat ttaaacatgt aagtccagca ggagcagcag
     1261 taggggtacc tttatcagat actttaaagc aaatatattt tgttgatgat ttagagcttt
     1321 ctccattagc ttgtgcttat gcaatggcaa gaggagcaga tagaatgtct tcttatggtg
     1381 attttatagc actttcagat gtatgtgata aggagactgc aactattata gcaagagagg
     1441 tttctgatgg tataatagct ccaggatata cagatgaagc ccttgaaata ttaaaaggaa
     1501 aacgtaaagg caactataat attgtaaaaa tagatgaaaa ttatactcct gagcctattg
     1561 aaacaaagga tgtttatgga attacttttg aacaaggtag aaatgaaata ttaataaatg
     1621 aagaactatt aaaagatatt ccaactgcga ataaaatttt tacagatagt gcaaaacgtg
     1681 atttaattat agctcttatt acattaaaat atacacaatc aaattctgtt tgttatgcta
     1741 aagatggaca agtaataggg gtaggtgcag gtcaacaatc acgtattcat tgtactagac
     1801 ttgcaggtaa caaagctgat acttggtatc ttcgtcaaca tcctaaagta ttaaacttaa
     1861 aatttaaaaa ggatataggt cgtccagata gagataacac tattgatgta tatctttctg
     1921 atgattatat ggatgtgctt gctgatggca tatggcaaaa cttttttgaa gaaaaaccag
     1981 agcctctaac tggagaagga aaaagagcat ggcttaaaac tttaacagga gtagcacttg
     2041 gttcagatgc atttttccca tttggagata atatagaacg tgcaaaaaga agtggggtaa
     2101 gttttatagc acaaccaggt ggttctattc gtgatgataa tgttatatta acttgtaata
     2161 agtataacat agtcatggca tttactaaaa atagattatt ccatcattaa aatactgaaa
     2221 gtataaagat aatatagatt aagtaaaaat ctacaacaag ttttgttgta gattttttta
     2281 tttgctgtat aattttaaat agaactttat ttttagatat tcaatttgga ggttgatatg
     2341 gctttaaata taagaaacta tagcctatta tgtaatcaag gaacagctaa atttaatgaa
     2401 gatgtagtcg gtataaatcc atttggggca tgggttttag atggtgctac aggacttaat
     2461 ggaaaaaatt tagtgtcaaa tgaaagtgac gcaaggtggt atgttcagtg gtggaatagg
     2521 tatttatata aaaatataaa taaagaagaa tcattaaaga gtattattaa tactggaata
     2581 atagaagtaa aaaaagagta tgaactaaga ttaaatgggt ttaaggttga aaaattagat
     2641 ttaccatcta gttctatagt tgttgtaaaa tttcatgaaa ataaagtaga gtatttttta
     2701 ttaggagatt gtgctttgtt tattaaaaat ggagattcca aagttataaa agataggagt
     2761 atttgtaaat ttgataatac tgtatttgat gaaatgagta aactttctaa cttggaagag
     2821 atggcatttg atgagattag aactagtgtt atggatacaa tagtaagcaa tagattgaag
     2881 aaaaatacga aagagggcta ttggatattg gattttgatg aggaagctgt ggaaaattct
     2941 atatatggat acattgaaat aaaaaaagat tttcaattga tgctaacgag tgatggtttt
     3001 acaagtgcat gtgatagata taatctaata aaagaagaag aattgatacg tatagcagaa
     3061 aaatttggta taggatatat acataataaa gtaagggatt ttgaagataa tgactataaa
     3121 gcggttaaag tgccaagatt taaaataaaa gatgattcat cttgtttata tttagatata
     3181 tataatgatt agactgtata aaactattta attttagaaa ggaggcaagt gaaaagtgaa
     3241 aattcttcat ataataacac aaaaaccaaa tagtacaggt agtggtatat atttgtctgg
     3301 tatgataaaa gggttcgaaa aaatcggtca taagcaagct gtaattgcag gtatagatgt
     3361 aaatgatgat gtaaattgtt ttccaagtga agtaagtttt tatccagtaa agtataattg
     3421 tggggagtta aattttcctg ttgtaggtat gagtgattct atgccatatg aaagtacaag
     3481 atataaagat ttaaatatag acatgataaa tagattgaaa tatcaattta aagtaaatat
     3541 agataaggct atgaatgatt ttaaaccaga acttataata tgtcatcatt tgtacctctt
     3601 aactgcattt gttagggaaa tagtaaaaga tataaaggtt atgtctattt gtcatggaac
     3661 ttgtttaaga cagttaaata ccattgattt agagaaagaa tacataatag caaatataag
     3721 gaaattagat ttaatatttg ctcttcatga aaatcagaaa tatgatatta taaagacttt
     3781 tggagtaagt gaaagtaaag tagttgtaat tggtagtggt tataatgatg atatatttta
     3841 taataaaaat tataaaataa aggatgataa aataaaaata gtatttgcag gtaagatatg
     3901 taaatcaaag ggattgattc catttataaa atctatatct aaattaaaat atagtaaaga
     3961 tttaatagaa gtcaattttg caggtactgg aagcgatatc gaatcatata atgagatagt
     4021 taaactggca agtaagtctc catttaaaat gaatttccta ggaaagttag aacagagaga
     4081 tttagctgag ctattcaaca ggtcacaaat atttgtgtta ccatcatttt atgagggatt
     4141 accagttgta gttttagaag ctttatcttg tggcacagat gtaatcacta ctgatattct
     4201 tggtgttaaa gagtggattg gtagtgagat taataattct ggaaagatag aatatatttc
     4261 tctacctttt atggagaaag aaggtattcc aaaagatgaa gagttatatg attttgaaaa
     4321 taatttgtat aatgcaatag attctaagat acaatcttta ttgaataact caaatgagaa
     4381 aacatctgta gatatgtcaa aaaaaacgtg ggatggctta gcatatagaa taaatgaagt
     4441 tattttaatg gatgaattgt gtttagtcta aaattattta aattataaaa aagtgtaact
     4501 atttttaaac tttttttaaa tgattttata ttatactaat actaaatata tttgttttag
     4561 ggaggtaatt tatgaggatt agtaaaaagg tattggcgtt aggtatttca gcattactgt
     4621 taagtgttag ttttccaaca tcaataaacg cactagataa gattgaaaat attcaaggag
     4681 tagataaata tgaaacagct gggcttattt cagataagca agattttaca acagcagttc
     4741 ttatcaatgc agattctaca atggtagatg ggattgctgc aagtggtctt gcaggagtaa
     4801 ataatgcagc tattttatta actaataagg atgatatacc agaagcaaca cttcaaagac
     4861 tgaatagagt tactaagata tatgtaatag gtggagaaaa ttctataagt aaagatgtag
     4921 aaaaaatgtt acttatgaga agaatgcaag ttataagaat tgatggtgta gatagagtag
     4981 atacaagtta taaaattgca ggtgaaatag aaaaaattaa aaacagtgat aagatgtttt
     5041 tagtaaatgg atttaaggat gaagctgatg cagtaagtgt tgcatctgtt gcttatagag
     5101 atggagctcc tataatattg acaaaagata taccttcagc agaagaagac acagacttag
     5161 acccatggtt tggagtttct cctgttccag tatatgctat tggaggagag tctacattaa
     5221 gtgattacat agtgagcaga tatagagcaa ctagaattgg cggtgttgat agatatcaaa
     5281 ctaataaaaa tgttattgaa aagttctata atggagcaaa agagttctat ataacaagtg
     5341 gagacgattt agtttatgct ttagttgcat caccacttgc aaaaaatgct cctgttgttt
     5401 tagtttcaaa taaaagtgat aagagtatat taagtggagc tagtaaagta acagcaatag
     5461 gtataagtga taagagtatt atagagcaat gtttggatgc tgtaaagaag taatggagtg
     5521 ttattagtaa aatgaatata tacattgaaa attagtagat tgttcatttt ataatggtat
     5581 aaaatatatg ggaagaggct atcaaatgga gataagcctc ttttttattg ataaaattat
     5641 gaaaatatac tttattttat ataaaaaaag tatctcatga actaaatgtc tattttgata
     5701 tactatctcc ttttcttata ttatatgtat tatatgattt tttatatgga ataaatttca
     5761 taagtattat cttcctttaa tttttctaac caaatcatct ctaaaatatc tagttcttct
     5821 aaatagtctt tctctgtttt gttatcatca tgttttataa gttctaaaac ttcaaatgta
     5881 aatgcttttt caccatattt tttccattca ttatttaatt cttttatcat acatgagcca
     5941 gcaccaagct gaaatttgag agagttaaat ttgcttttta tgtcattact ttttccaagg
     6001 taaatctttt tagtaatatc attttttatt atgaatacac ctgtgtcaaa tttcatttct
     6061 ttgtataatt gttttaattc acgttttcta tccatgattt tagatacctc tctttataat
     6121 tgatatgtaa tatgtaagtt taaaaatata ttacatatca attataaaga gattaatttt
     6181 atatgtaaat agtttttata aatttaatta tgaattagtc atcaataaac aagaaattta
     6241 gctgtagaaa tccattataa aggctattat aaattttaat atgctaaaaa tataaaaaga
     6301 atagttgaca gtatagaaaa tatggaataa actaaaagtg taatagtaat acgaaattat
     6361 atattacatt gaaaggtgtg aatgattatt atgaataaaa aaatagaatt aatagggaat
     6421 tttgcaagtg attggattag gtatagtgat tatgaatata aaattactga agaagatgaa
     6481 ttatatatag ttccaacaga agatgctgtg tttaccatgt ataacccatt tgatgtagca
     6541 gatgatataa tagtagatat aattcgcata ggacaagagg cattacaaga tatatcaaaa
     6601 gagacaaaag aaatgaaact aaagaaagaa gtacttgaat ttgtaaaaaa gtatggatta
     6661 ttgggtttaa tatattcaag tgtatataat agagatatag ttggagaaga aagagtactg
     6721 atgattgaaa agaattatat tacaaaagag aatatattga gtacagatga atatgtaaac
     6781 atgtttatac catttgcaag tgatgaagat gtgtatttta aaaaatatag aaggggtata
     6841 gatgtatcaa agagagaaga atctccaaag ttttttggaa agagaccatt gattttagat
     6901 ttagtatttt caaagttcta cactgaacaa ctagattgga ttgtaagttt tgctaaagat
     6961 atgtgtaagc attttaatca aatttgtgta tataaaaata catctaatct attaactgaa
     7021 agtgttacta ttatgtctgg agggtttaat cctcaaaaga taggattttc tataagccaa
     7081 ttagataaac caattattgc atgggagttt gattctttga aaacagctat tgagactatc
     7141 tatgcctttg caattacaag tgaaccttct tctttaaata aatgcaagta ctgtggtaaa
     7201 atttatattc ctacaaattc taaatctcaa tactgttcac cttcttgtag aaattgctca
     7261 aatgtaaaga aaagtagaag tagagccaaa aaagataaat aaaattaata aatatatcat
     7321 tgtttagatt ttagtaaata actactgaat taaaagttgt tatagaaaat tatatatcta
     7381 aatgatggta tttttttgtt gagaaaattg aaaagtttaa ttgagatagt aattttgagt
     7441 tgacaaaaac cacttttaac actactatta taagtggttg gtaaaataat atacactaat
     7501 ttaaatatag cataaaaaac aaaaaaagtc aatgaaaaaa ataaaaaagg agaatttaaa
     7561 aatgatatca cctattattt tgagttctat aaatcagaat ttaaaggaaa tagaaagaaa
     7621 tgaactgctt gaaactaata tagaatctgg agattatggt ctagctcttt ctgaatctga
     7681 tgtaaaagat ataataaatt cgagagataa tactttaaag gggtatggaa gaatagaatt
     7741 agatataaaa gttacaaaac agttaataga aaatatatat acatcacaat atacaaatgt
     7801 ggacaattat ttagaggcta ttaatgatat gcaagaaatt ttttattatt taaaaaatga
     7861 aactgatgac aagatatgtg atgatgagat tatagaaata ctgggtgaat tttatgaaaa
     7921 attttcaggc aatatggaca atgtaagagg agaagcagac gaatttgcaa agaaatttaa
     7981 atttggagaa gtttaatata tgataggaga gattttgtat gttaaataat ttaagtaata
     8041 ttaatataaa cgaaaataat ttaataaaga accaatattc catatctcta cttaaggaat
     8101 gtctaaattg taaagtgata gatgaaagag aagtatataa tatacaacaa gaaatatctc
     8161 taatacttat ggatttgatt aaaaaatata caaatggtca aagcacatca gtaaaaacag
     8221 aagttgccga aaaactgttg atttcaatat ggtatgctat tgatgcatat ataaataagc
     8281 ttggagatat agaaaaaaga gttgtggagt tgactgctaa gaatgttaaa aaagtgtacc
     8341 ttggaggcat tgaaatatta gaggaagagt tagttaggct gaaagatttt tataaactaa
     8401 tgatgagtag aaaattggat actaattcaa tagcatataa tgataccttg atggagttat
     8461 caagtttctt taaaagttat attgtaaaat ttgaagctca ggacgttcca gtaagtatag
     8521 attatccatt ggcactagat gatatggatg ttaagggtat ttattatgtg aagaattata
     8581 ttgagagtat agatatagaa aatagatttt gtaacctatt taagaaaaaa gatagagaaa
     8641 aattatttta tgactatggg gtgatttata aaatagattg tgatgttata cttgtaaatt
     8701 tatttgaact tattataaat aattgtattt tttctactat tttaggaaat gaagtgatta
     8761 atttagaaat ttcaaaatat gagtatgaat ttttagaaaa acaatttaaa aatatgtaca
     8821 ataatattac tgaagataaa gttataggct ctgaagaaag taatataatt gaattgattg
     8881 aagtagatga tagttttaac actgaagtag atagtaattt ggattataat gattttgaag
     8941 aaattaacag agaaattaga ataaataaaa aaataacttt actacttatg gaagcaattg
     9001 aagtattaat aaaaagatta agcatagaag atgaagatgt aataagatat attaaaaaat
     9061 atgaaaagat atttattgaa tctgtgctaa gctcaataaa aagtaacact tttaaaagta
     9121 tggtaactgt gagtaaaaat aaaaatgaac acttagaaga ctatattgtt gatgatgaaa
     9181 gtaaattgga tgatgaatcg tttagagaga tattcaatga gatattagat agtacatcta
     9241 taattgaaaa aataagaata ataaaggaaa atatcaatgc taaaaaagac tttatcgata
     9301 ttcttgaatc tgaatgttta tttggagaag agtatttaat gttgtttgca tcattaagtg
     9361 aattagaatt ggcaatattg ggtagtgtag tattttatga agatattaga atgagagaaa
     9421 ttaatatatt agaatttatg ttaaatgaga aaactgaaac tactttatgg aagacagagt
     9481 acattaaatt tatgaagagg caaagtgaag ataagatata ctccatagaa gagcatatga
     9541 ataaaattaa ttaacaaaaa attgtgaata aatagaatgt tagtatataa tataatctgt
     9601 aaagcatatc aaaaatacat atatttggag gaaaattatg aaagagtata tcataaataa
     9661 tgttgaagaa ataagagaag aattaattga tttatctaag aaaatatggg aaaatccaga
     9721 attagccttt gaagaaaagt atgcaagtag cattcaaaaa gaatacctaa agtcaaaagg
     9781 atttaaaatt gaagaagtag aaaatttacc tactggattt atagcaagtt ttggagaggg
     9841 taaacctgtg attggcatac ttggagaata tgatgcttta ccagagctat cacaatgtgt
     9901 gagtgcagaa agaaagccat tggtagaagg aaaagctgga catggatgtg ggcataacct
     9961 gttgggtgta gcaggtgtag gagcagttgt ttctataaaa aaattaatag aagaaaataa
    10021 atttaatgga acaataaaat attttggttg tcctgctgaa gaagaaggtg gaggaaagac
    10081 agttatgtgt ataaatggct gttttgatga tgtagattgt gcatttacat ggcatccttt
    10141 tgatataaat gcaccttgga gaggtggaag tctggcaaac ttatctgtaa aatttaaatt
    10201 taaagggata acagcccatg cagctcaagc tcctcataat ggtagaagcg cacttgatgc
    10261 tgttgaaatt atgaatgttg gagctaatta tttgagagaa catgttattg actctataag
    10321 aatgcactat gttataacta atggtggtgg aagacctaat gtagttcctg gatttgctga
    10381 aagttggtac tttattagag gtaaaaaagc aagggatgca gaacatgttc ttgatagatt
    10441 aataaaagta gctcaaggag cagcaatgat gacagaaact gaaatggagt ataaggttac
    10501 agatggaata tatgactata taccaaacca atgtttgact gatttagttt atgacaatat
    10561 ggtttttgtt ggttgcccta aaaatactcc tgaagaagaa gaatttgcaa aaaaattgtg
    10621 tgatactctt actagagaag aaagattagg tgtagcaaca tcattacaaa atgataaatc
    10681 tattgtagaa agttatatac acaaggaaat agttgacggt gacaaagata aaggactagc
    10741 tggttctact gatgttggag atgtaagtta tgtaatacca gtggctcaat ttgcaatggc
    10801 agcttggcct gttggaattg caagtcatac atggcagtca tgttcatcag caggttcaaa
    10861 tataggattt tctgctatga taaattcagc taaagtactg gcttgtagtg cttatgatgt
    10921 atttatgaat actaaaatta ttgatgaagc aaaaattgaa tttgataaat cattagatgg
    10981 acaaaaattt aaaccattgg tataattttg gtaataaaat atttctgggg ggaatagcta
    11041 tgagagaaaa agttgaaaaa gtgcttgagg aaaagataaa accagtatta caaagagatg
    11101 gtggagatgt agagcttata gatgtaaatg aaaatggtgt ggttttagtt agactacaag
    11161 gggcatgtag tgggtgtcca ggagcgacta tgacaatcaa agctattatt gaaaatgttt
    11221 tggtgagtga agttccagga gtaactcaag tattaggtgt ttaatagtta gcgtttatta
    11281 aatgtatgtt atacataagt atttaataaa gaagtaaaaa aaatagtgaa gcaatatata
    11341 tgaaaaagta tgataaagat aataaaacat tatagaaata atgaaatatt tcatatgtat
    11401 atgaaga
//
LOCUS       DAMJVX010000113        11335 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMJVX010000113 DAMJVX010000000
VERSION     DAMJVX010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.118"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            23..334
                     /locus_tag="PC407_002602"
     CDS             23..334
                     /locus_tag="PC407_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2498037.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      427..519
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            619..1413
                     /gene="thiD"
                     /locus_tag="PC407_002603"
     CDS             619..1413
                     /gene="thiD"
                     /locus_tag="PC407_002603"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDF2498038.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1437..2225
                     /gene="thiM"
                     /locus_tag="PC407_002604"
     CDS             1437..2225
                     /gene="thiM"
                     /locus_tag="PC407_002604"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDF2498039.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGIYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            2248..2880
                     /gene="thiE"
                     /locus_tag="PC407_002605"
     CDS             2248..2880
                     /gene="thiE"
                     /locus_tag="PC407_002605"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2498040.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITERYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEATCAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            3019..3321
                     /locus_tag="PC407_002606"
     CDS             3019..3321
                     /locus_tag="PC407_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2498041.1"
                     /translation="MIKILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRVPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3387..4136)
                     /locus_tag="PC407_002607"
     CDS             complement(3387..4136)
                     /locus_tag="PC407_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2498042.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4130..5092)
                     /locus_tag="PC407_002608"
     CDS             complement(4130..5092)
                     /locus_tag="PC407_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498043.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVKMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5096..5554)
                     /locus_tag="PC407_002609"
     CDS             complement(5096..5554)
                     /locus_tag="PC407_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2498044.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            5910..6266
                     /locus_tag="PC407_002610"
     CDS             5910..6266
                     /locus_tag="PC407_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2498045.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6338..7054
                     /locus_tag="PC407_002611"
     CDS             6338..7054
                     /locus_tag="PC407_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498046.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7047..7871
                     /locus_tag="PC407_002612"
     CDS             7047..7871
                     /locus_tag="PC407_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2498047.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQNILFFGMLGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIVLITMIYGFLISQTATLFICKYIFNFIFRNIPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIINLFRKYYNLIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKVSL"
     gene            7883..9082
                     /locus_tag="PC407_002613"
     CDS             7883..9082
                     /locus_tag="PC407_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498048.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYANILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQDIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDNKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            9091..9903
                     /locus_tag="PC407_002614"
     CDS             9091..9903
                     /locus_tag="PC407_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498049.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFIFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVLEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            9914..11182
                     /locus_tag="PC407_002615"
     CDS             9914..11182
                     /locus_tag="PC407_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498050.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNKNPKSRIRNDYLNIDKKYSDFVNDKG
                     KELIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     YQQNINEIKFSIGSKLYLEIVNNKSKNGDIEGHDSYSNLIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMIKNNKLNLIVTDMSNSIINLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGMKNSISGELKGFKGIVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
BASE COUNT         4527 a         1170 c         1810 g         3828 t
ORIGIN      
        1 taatgttagg atgtgattta aggtgtattt tacatatatt ataagatgta aagatgattc
       61 tctatacact ggatatacca gtaatatagt tagaagaatg aatgaacata agttaggaat
      121 taattcaaaa tacacaagag caaagggatt tgaaaaacta gaagtttatt ttgtgaccaa
      181 cacaaaaagc aatgctatga agttagaata ctatataaaa aaattaacta gaaataaaaa
      241 attgtctata ataaaaaacc caagtatact tataaattta attgataata aagaagatta
      301 tattattggt aaggaaatag aacaattaac ataaatattt atagtaacat tataatgggt
      361 gcatatagtg taaaaacttg acaataaata ctattgtaat ataatgaagt tataaatata
      421 gtaaattaaa atagctaggg gagccaagag ctgagaggta taccgaccct tacacctgat
      481 ctggataata ccagcgtagg aaagcttagt aaaaattcaa gtatgtatta tttgtgatat
      541 atgctatttc taagctatac ctattgtagg tatagctttt ttaattaagt tatttaaatt
      601 aaactttagg aggcaacaat gagtaattat aaaataccaa cacttacaat agctggctct
      661 gattcatctg gaggagctgg gattcaggct gatttgaaaa cgtttagtgc aattggtact
      721 tatggtatga gtgtaataac agctataaca gcacagaata ctaaaggagt ttttgctgta
      781 gaggatttaa ataagaaaat aataaaaaaa caaatagagg cagtctttga agatatacct
      841 ccaagagctg taaaaatagg aatggtttca agtcctgaga ttatacttga aatagttgag
      901 aatctaaaga aatacaatcc aaaatatttg gttgtggacc cagtaatgat atctaaaagt
      961 ggatattatc tattaaagcc agaggcaaaa gaaaacttaa ttaagtattt gataccactt
     1021 gcatacataa ttacaccaaa tataccagag gcagaagaaa taacaggaat aaaaatacat
     1081 aatgtagatg atatgaagag agtaggagaa gaaatactac aacttggacc taaatttgta
     1141 cttatgaaag gaggtcattt agatggagaa gcagtggata ttttagttgg taaaaatata
     1201 tttaaagttt ataaaagtga aagaattgat aaaaaaaata cacatggaac agggtgcaca
     1261 ttatcttctg caataacatc ttatctagca ttaggatatg aaataacaga agcagtaaat
     1321 ctaagtaaaa tctatataac agaagcaata aaaagaagtt ttgatatagg tcatggagta
     1381 ggaccagtac atcattttta taaatttgag taataaaaaa atattggagg tagaatatgt
     1441 ataatttaat taaagatgta aaaaaattaa atccattggt aattcattat actaataatg
     1501 taactataaa tgattgtgct aatgtgactt tagctgtagg agcaagtcct cttatgagtt
     1561 tttcatatga ggaagtagaa gaaatggtaa gtgtagcaaa ttctgtagta ataaatatag
     1621 gaactatgaa ctcaaatatg ttggatttat ttctattagc tggaaaagca gcaaataagt
     1681 acaataaacc tgttgttttg gacccagttg gagtatttgc aagcaaagcg agagcagaac
     1741 ttacaagtag acttttaaat gaagtcaaat ttagtgttgt taaagggaat gtatcagaaa
     1801 taaagtttat aggtggcttt aatgtaaggg gtaaaggtgt agattcattt gatgaagaag
     1861 aagattctac tgagataata agaaagattg ctgaaaagct tgaatgtgta gttgtagcaa
     1921 ctggaaaaat agatataata actaatggta aaggtatata taaaataaac aatggaactg
     1981 ataaattaaa aggaattaca ggaactggat gtatgactgc tagtttgatt gcaagtttta
     2041 tggctgttac tgagaatata ttagaagcag caacaatggg tgttcttaca atgtctttaa
     2101 gtggggaatt agctaattta aataatccac caataggtac atttaaagaa aatcttatga
     2161 atgctattta tcaaatggat atagatactt taagtaaaaa ttctaatatt gaatttttaa
     2221 attaataaag agtaagggag acttaaaatg attgataaag aatctttaaa aaaatgctta
     2281 aagttgtatt tggtaactga ctctgaaatg cttaaaggta gagactttta taaatgtctt
     2341 gaagatgcaa tatcttctgg aataacaact gtacaactta gagaaaaaaa tgcttctgga
     2401 agagagtttt taagaaaagc tatgaaactt agagaaataa ctgaaagata tggtgttaag
     2461 tttataataa atgatagagt ggatatagca cttatttgtg atgcagatgg agtacatgta
     2521 ggacagagtg acattgatgt gagagaggtt agaaagctta taggaaacaa taaaattcta
     2581 ggagtatcag caagaacttt agaagaagct acttgtgcaa aaaatgatgg cgcagactat
     2641 ttaggtgtag ggtctatatt cacaacatct actaaattag atgcaaaatc agcttcgttt
     2701 gaaactgtta aagaaattaa agaaaaagta gacatgccat ttgtgctaat tggaggtata
     2761 aatttagaca atatagataa acttaaatgt ttagaaagtg acgggtatgc tataatatca
     2821 gctatattaa aagctgaaga tatctctaaa gaagtggaaa agtggacatt aaaaatataa
     2881 aattggctct ggtagtgaaa aaaatggaaa aatttgatat aatctaagag taaaatttaa
     2941 gcaattataa atgataaact tttctacata tactttttaa tcaagatata tgtagatttg
     3001 gttaaaaagg agctataaat gattaagata ttgttagtgt gtgttggagg catgtcttct
     3061 accctattgg taaataaaat ggagaaggat gcaaaaaaaa gaaatataga ctgtaaaata
     3121 tgggcagttg gagaaggaga tataaaatcg gagcttgata actttgatat tttacttctt
     3181 ggaccacaac ttagatttat gcttgatgac gtaaagtcta tagttggaga tagagttcca
     3241 gtatctataa tagatatggt aaactatggt acttgtaatg gtcatgctgt gttaaattcg
     3301 gttttagaaa tattaaaata aataataaaa gtggattcta tataatagag tccattttta
     3361 ttatttgtta ttaataggct taaaacttag cttaccattt ctttttttaa tctccacata
     3421 gcaagtgaaa aacttgaaca gcacaatatt aaaaatagta ggataacagg cagtatatct
     3481 ccaataagaa tagtctcacc aaatattaag ctacgtaaag cgttaataac atgggtaaat
     3541 ggattgagca tcactgctac ttttaatcct ccagataaac tttctatagg gaaaagagct
     3601 gaacttaaaa agaaaatagg aagtacaatt gcagtcataa ttgtttcata aactatttca
     3661 tttggcaata ataaacttat agaataagct agacttgata aaaagaaggc tgtcataaat
     3721 atcagtacta tcataagtag gattccacca atgccagatt ctatccttac tgagaagaat
     3781 atacttacta tacacaaaat agttacttct atgaaggaaa ctaagatagc ttctaataat
     3841 tgaccaagga cgattgaata cctactgata ggtgcaatta agactctata gaaacttcca
     3901 ctgttcttca ttataaagtt tataatacca ccactactac aagaagaaaa cacaaccagt
     3961 acaatgatac ctggtagtat aaaagcagta taattattga tatttatatt attcatagtt
     4021 tggtttgcaa tagaactata aagcacaagc caaagaaaag gttgtaatat agtaactaca
     4081 aatgagattg gatttttaaa acgccacttc atactacgcc ataagatatt taacattccc
     4141 attcctcctt taagcttttt ttagaacttg taagtgctaa aaatacatct tcgagacttg
     4201 gctctacaat ttctattgca tcaaattcta tatcatgttc taaaagccat ttatttatta
     4261 aagtaaaagc agttctacta tcgtttacac ttataaaaat tgaattttct cgtacgctca
     4321 taaactttac taaaccagta cttttaattg aatctttata tttttttata tcttcagtat
     4381 tatggaaagt tatacgtagt atattttgtc taatgtaact acgtaaactt gatggtgttc
     4441 cttgtgctaa atcctttcca ttcttcataa tacaaatatt atcactcaat tgttcagctt
     4501 cttcaagata atgagtagtt agaaatatag ttgtgccgta ctcatctcta atttttaata
     4561 acatatccca catagatttt cttgaatcga catccatacc aacagttggt tcatctaaaa
     4621 ataggatttt aggagatgat accatattca tagctatgtc caatctacgt tttacacccc
     4681 ctgaatatga tgaagtagga tattttaagt aactagataa gtcaaactta tcaattaaag
     4741 agtctattct ttgttttgca atctgtggtt ctactttata cattttactt tgaaatacca
     4801 tattttccat aagggataaa tgttcatcaa tagagatttg ctgtgctaca caagcaattt
     4861 gagtacgtat ccaagaagga ttatctacta aatcttttcc aaacattttt acatttccag
     4921 atgtaggttt gtaaaaggta gtcaaaatat ttataagaga agatttacca gcaccattag
     4981 gaccaagtaa agaaaaaatt tctccagcat ttacattaaa gcttagacca tttaaagctt
     5041 tcacaccatt tttatattct tttactaaat tgtctatttc aatcgctaac atatattatt
     5101 cctcctcttt aagtggaaat attatctctg taatatagtt atctgggttt ccttttatca
     5161 acatacctgg tcctttaata aatacttctc tccaaggagt ttgtattttt atattatttt
     5221 catgaatata tttaaatatc atttcatacg tttttggcaa attactataa gagcctgtgt
     5281 gagtaaaaaa taaagctttt gttttaggaa attcttttgt agttataccc atacgatttg
     5341 gagtttcagc agttggaaca cataattcca tttctgcaac accagttttt tggtcaacat
     5401 catagtagca aaagaatggt gcaccgttag actttccttt tatagcttta aatacatttg
     5461 gaaaatactt tgttgcctca gtcataggtc cattataacg catggtagcc actgtgactg
     5521 attcgatatt tttgatttca attttgcaat tcatttgttt acctcctaat gtgtgttcat
     5581 attcttacta gatttttaag cattaataca tataagattt tgattagttg aaaacatata
     5641 atcttatctc gtttgcttgt tttcaagtct aacataacat aaaatatata tcctctcatt
     5701 tgatgctcca ttttgtaaag ctattattta agctttactt tagatttttt agcaaattga
     5761 aataaagcaa tttataaaat aagtatttac aaattaagtg tattaatcgt ataatacact
     5821 taataggata ctagataaag tctgaaataa aggcgtattt ttttaaagta agtgtactat
     5881 cgtattaata cactagaata ggaggaatca tgaattttga attagataat acaactccca
     5941 tttatttgca aatagtaaag tatataaaga gacaaatagt aacaggagag ttgaaaccag
     6001 gagagacaat accatcaaga agagaaatgg cacttaatct aaaagtaaac ttaaatacag
     6061 ttcaaagagc atataaggag atgggtgata tgaatataat aaatactttt aaaaattatc
     6121 aaagcagtgt aacagttgat gaaaatatat taaaaaattt aaaattagag cttataaatg
     6181 aatcactatc tgtttttata gaagatatga aagcaataaa tgttagcaaa gaagaagttt
     6241 taaaaataat agaagataag tattagtttt ttaacaaata gaaaacttag tttagaaagt
     6301 ttatttataa gcatattgga tgtggaggat agtaaatatg atagaaataa agaatgtcag
     6361 caaaacatat aaaaggatgc aagggcttaa aataagaaag atagaagctt taaaaaatgt
     6421 aagttttaat atagaaaagg gaaaaataac tgcattactt ggtataaatg gtgttggtaa
     6481 atcaactatg ttaaaagcaa tagcaggact tataaacatt gatagtggag aaattcgtat
     6541 agatggagaa aaaataaatg aaaaggtata caataaattg gcttttgttc cagatgtaca
     6601 atctcacttt agcaatacaa caataaaaga aacatttgaa tttatggaaa ttttttattc
     6661 aaagtggaat aaagaaaaat caaaagaaat gatggacata tttaaattgg atgaagatga
     6721 aataattgat aacttgtcaa aaggaaacat tgcaagagta aaattaatac ttggattttg
     6781 ccaagaccca gagtacatac ttcttgatga accatttaca ggaattgatt tgtttaagag
     6841 ggaagagttt ataggagtaa tagcgcaata tatggaggaa aatcaagcca ttatcataac
     6901 tactcatgag atagtagaga tagaaagttt agttgatgag gttgtaattc ttgatgaagg
     6961 gcagattata acttcattta atgcagaaga attaagagaa agagaaggta aatctatatt
     7021 agataagatg agggaggtat ataagaatga ataaacttct atatctaata aatgtagatt
     7081 tgagaagaag caataagttt tatatagctt atatatcatt tttttcatta attacactta
     7141 gtttaaattt attcgaaatt aataaattta aaaattctaa atttattatg aacttagcaa
     7201 tagaagattt tggaggtata ttttatggac tagggatact tagtaatctt ggttttatcc
     7261 aaaatatatt gttctttgga atgttgggac tttttatata ctatattttt atgtggaaaa
     7321 gagagttctt ttcaaataat caaagtattt atacacttat gatgttacct caaaataagt
     7381 ttaagatttg tatatctaaa tcaatagttt tgataacaat gatatatggt tttttgataa
     7441 gtcagacagc aacattattt atatgtaaat atatatttaa tttcattttt agaaatattc
     7501 ctataatcaa tatgaacttt gccaaagatt tatattattt aaacttgaag agtataccaa
     7561 ttgattttat ctcttttatg gctatttatg tatttttact acttatagct atttctattg
     7621 taaattgctg tatattattt atcttttcct atataaataa attttatgta attttaatca
     7681 ctttgctgat tatagcaggc ttatattttt caacaatata tataaatata atcaatttat
     7741 ttagaaaata ttataatctt ataggatttc aatttaacaa tttagtagca gggttattaa
     7801 gttttttaat tataatgttt attctaaatg gaatatctta tttgctaatt aaaaagaaag
     7861 tatctcttta aggaggggtg gtatgaaatt aaaatctaaa atatctatac ttattatatt
     7921 tttgacttta gcaatatatt ttagtatagg tttattaaat aatgaagata atttaaagat
     7981 aaaaactata aaaggtgact ctaatgaatt agaccatata aacataaatt ttggatatgc
     8041 tttgaatgga tatataaatt catattatac aataaatgga agtaacaaaa ctaaaaagac
     8101 tgaattaata aaactacaaa aggatagtaa gaacacaaat aatttaattg atgtatctag
     8161 tggttcagat ttaattgctc atgttggatt tgaaatgaat gaggagtcaa aagatataaa
     8221 ctttgtgata gatgagaaaa aagaagattt agaaaaaggt gtatttccat cagaaggtag
     8281 tgataaagaa attgaatatg aaaaattaga tatgaataat tttacaagga caagatataa
     8341 agtagataaa agtacagatg attattatgc aaatatacta gcatcaaaaa gatataaaaa
     8401 atacttatat gtatctgtac tttatgctac aagtgattca tcagaaaata acaaagaata
     8461 ttcaagtact atagatataa taaaaattaa tccagaaaat aaaaaaatca agactataaa
     8521 aagtataaat ttaagtaatt atttttccga gaaataccaa gacatagaga acataggtgt
     8581 aagttatgct agcaaatatg aggataagat gtatatttta gtaaatgtac taaatacaaa
     8641 cataggtgat aatagtaatt ctaaatttca tttacttgag tataatcttg aagaaaataa
     8701 ggaaagcata atggaattaa aaactgatag caaaatggct ttagcaaaag caaagttaga
     8761 agctaataaa ttatatatgg taatgtcgaa tgattatgat aatagttcaa ttacagagtt
     8821 aaatttactt acctataata tattagataa aagcttagat gaagataaat atgttttaga
     8881 taatagagaa aaaagaggaa ttagaagtat tgaaattgat tcaaataaaa tcgttgcata
     8941 tacatcaagg ctaaactcta tattatcttg ggataataaa tttatgctat atataataaa
     9001 taaaaattct aaggaaattg tatatgaagg agaattacaa tttaacagtt caaatagtat
     9061 gttagaattt gaaataaagt aggtgagttg atgaaagatt taataagatt attcgaattt
     9121 gaattaaaaa gaaatataaa gaattatata tttataataa tttgttgttg ttcttttgta
     9181 attttaaaca tagtaaaaaa tttaaatgac tataattaca taatagaaaa tgcagttaaa
     9241 agtgaacagt taacaaaaat cggaaatacg atagaaacgg taaatgttac aggtttctca
     9301 agctttagaa atataatggg ttctacagaa tcgtggttta tatttggtat aattgcatgt
     9361 atttgctatg ctttttttat ttggtataga gactttaatg gtaggagtaa atctatatat
     9421 acacttataa tgctacctaa aaatagaatc aatatttata tatctaaatt attaaatata
     9481 ttatttctag tgtatagcta tacagtagta cttacaatat ctttgtttat tgcaagtaag
     9541 ttgttgccta gacatatgct tggtaatgtc acaaactatg gttttgtaca agaaacaata
     9601 tatgagttaa aaatgttact tccatatagc tttgaaatac tatttgtaga gtatatattt
     9661 ttattgatag gatttgtatc tgtagtattt acatcaattc ttataaataa atctatttat
     9721 aaagtaagca ctttaataag ttttctattt ttagttttgg aaattttagt gtttataatt
     9781 tctatagaat ttattatacc ttatgaatac agtgatgtat ttactgttct atattcaagt
     9841 ataaacatat ttgtatgtag tcttatatct aataaacttt tgaagcaaaa gattgacttt
     9901 taggagggat attatgaaag caaaaaatat aggaaaatat acaatatgtt ttataatagt
     9961 atgtttttta atgattacta tgaccttaat attgagttat tttaataagg ataaactatc
    10021 tgtaaaaact ttaaaaggca atgtaaaaga tatagggaga gtaagtgtag tatatacacc
    10081 taatataagc aaattttctt atgaggaggt aattataagt aaaaatggag taagaaataa
    10141 taaaaacccc aaatcaagaa ttagaaatga ttatttaaat attgacaaaa aatacagtga
    10201 ttttgttaat gataaaggaa aagaacttat atataaagat gatttgaata taggagaagc
    10261 gtatacctct gttaagtctg atttagtaca aggaatgaat gctttaaatg attgtataaa
    10321 ggtttcaaat aaaaatttaa aaagtggggt agaagaaaat tttgatatta atttaaataa
    10381 gtctgatttt aaagaaaaca gttggaaaac cttgtttaat aaaatatata aaggcaattt
    10441 atatatggta attagtgaaa ctaatatttc aggttctaag gtcaataata tttgtatatt
    10501 aaagatagat ataaaaaata agtcttataa attattaaat agatttaatt taggattaca
    10561 gaatggatat caacaaaata tcaatgaaat taaattttct attggaagta aattatactt
    10621 agaaatagta aataataaaa gtaaaaatgg agatattgaa ggacatgata gttactctaa
    10681 ccttattata tatgatatag aagataacac ttttaatata agtaataaat ttatggataa
    10741 tctaaacaaa gaaaagaatg caaatccaaa ggagattggc ttattaagta aaactaagct
    10801 agattatatg ataaaaaata ataaattgaa tcttatagtt acggatatga gtaattccat
    10861 aatcaatctt gtttatgata ttaattctaa tgagattaaa ttagaagatt atgagatact
    10921 tgatttaaaa gtagattata atgaagcatc atctggagtt aggaaagtta agttactgga
    10981 tgataagatt tattctattt caggtatgaa gaacagtata tcaggagaat taaaaggatt
    11041 taaagggatt gttgatgtag tcggaactaa gccagtaaaa tttcaagtat ttgatatgaa
    11101 acttaaaaag aatatttatg aagctgagct aataaatgga gatataagtt tacaagaaaa
    11161 gttatttttt gttgcaaatt aagtattaga attaatttta gaactttaaa cttttatatg
    11221 tatcacagat aagtataagt aagggagtgt ctcaaaataa actttttagt tcatgagaca
    11281 ctttttttat atgaaatcaa atatattttc attgtttaga tttattttga taatg
//
LOCUS       DAMJVX010000114        11306 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMJVX010000114 DAMJVX010000000
VERSION     DAMJVX010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.119"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            453..1313
                     /locus_tag="PC407_002616"
     CDS             453..1313
                     /locus_tag="PC407_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2498051.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1303..3174
                     /locus_tag="PC407_002617"
     CDS             1303..3174
                     /locus_tag="PC407_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2498052.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRLTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEASNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3194..4648
                     /locus_tag="PC407_002618"
     CDS             3194..4648
                     /locus_tag="PC407_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2498053.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLMDLYLKYSETVI
                     RRFDDKVKYWITFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            5672..6880
                     /locus_tag="PC407_002619"
     CDS             5672..6880
                     /locus_tag="PC407_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498054.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNVLPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFRLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            6911..8290
                     /locus_tag="PC407_002620"
     CDS             6911..8290
                     /locus_tag="PC407_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2498055.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELSAY
                     GHGCGHNLLCTAHVGAVVGLKREMLENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            8396..9586
                     /locus_tag="PC407_002621"
     CDS             8396..9586
                     /locus_tag="PC407_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2498056.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEESTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEIIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(9714..10778)
                     /locus_tag="PC407_002622"
     CDS             complement(9714..10778)
                     /locus_tag="PC407_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498057.1"
                     /translation="MFLKLIFKYNTLDFSRAYFDLTDWADNPKDISYYLSNGTFGDLM
                     DSIDENASKMSLSDIAKLEKKIAKKHINLWNEGKIDFSTTRFSSIVPMLQNAGVNFHF
                     IYPDIDLLKNTFNNLIKDIHLHRMKGNQPSVIYITVKDTNYDDSTLDQVYKILNSIKK
                     DKLTDFIIEKNSNSFKIFTKCETIKSLTKNFTACYIKESIENSTNIKVCVGYGIGNDI
                     KQAMSSAISANKESKINPLNYSFLVNENYETIGPLLCDKLLTVSNKVTPHINTIAKSS
                     KLSTLTIQKIISVTEILQRDEVTPKELSSCLNVTVRTANRFLSSLLKSGNATILYEKQ
                     NSTKGRPERVYKLFISENKY"
     gene            complement(10926..>11306)
                     /locus_tag="PC407_002623"
     CDS             complement(10926..>11306)
                     /locus_tag="PC407_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2498058.1"
                     /translation="QRNDFLQKLSTKLIKENDIICIEDLQVKNMIKNRRLSRLISDVS
                     WSEFIRQLEYKANWHGRKIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNET
                     HDRDINASINILKEGLRLITIQNK"
BASE COUNT         4177 a         1301 c         2011 g         3817 t
ORIGIN      
        1 aactaaattt gaagctttac ctattaatat atcttaatag tgattaaata aaactaaact
       61 taagaattta tttattaaca tacagttcat tatatttaaa atcattacta tgataattca
      121 taatagtaaa atagataatg gttcagacaa tattgtaaat attagtgaca gtaaaatagt
      181 atcaatttag tatataaact ttgccagata atcgcataac agagcttgta gttatagaat
      241 aaaaaaatat ctaggaaacc gttgacagat ttatatggat agtataacat atagaatatg
      301 aaattaaaca aaaaagaatt ttaattatgg cgacgtagtc ataattttga gtatgttact
      361 gattagcagg cagaactcaa gtatatatgt gaaaaataga gggatactct atacatatat
      421 gcttgagttc tttttattga aaggaggaat atatgaaaat aattcagatt ttaaataata
      481 atgtagcctt agtaaaaaga ggtaaaaatg aaattattgt atttgttaaa ggaattggct
      541 ttaaaaaaag agtgggacaa gtaataaatg aaaatgaaat agagagaaca tatatactag
      601 attcatacga tatgctagaa cactttagtt atctactaca aaactcagac tccaataata
      661 tagttcttat taatgaaata attgaatatg gtgagaaagc tttaaatata aagtcaagtg
      721 attatctaag cttaacctta ctagaccatt tagagttttt acttaaaaga tgtgaaaaga
      781 atcaatttat aaaaagtcct ttggtatgga atgttaaaag attttatcca aagcattttg
      841 aaattggaat gcatgcacta aaattaattg gtgatagtaa gggaattaat cttcctgatg
      901 atgaggcagt atcaatagca cttcattttg taaatatgga ggtaaataaa gagtctcatg
      961 atagtacaat tgtagagttg agaactttag cagatatagt ttctataata aaatatcatt
     1021 ttaatgtaga gctagatgaa acttcaacaa attatatgag atttactact catctacagt
     1081 actttatcca aagaattatg tcagatgaag cttcaataga tgaagataat tctatagatt
     1141 tatataaaca agtaagtcaa ctttatccaa agtcatttgg aagtgttcaa aagataagaa
     1201 aatatataaa atcacaattt aatgtcgata tatctataag tgaagaaact tatttaatgt
     1261 tgcatataaa tagggtgact caaagactgg aggcaaaatc agatgaaata taaagattta
     1321 aatgaaaata taataaaact tataggggga aaagaaaata taaagtcggt agcgcattgt
     1381 gttactcgtt taagacttac tttaaatgat agaagtatcg ctcaaacaga agaaattaaa
     1441 aaattaaaag gagtaattga tgtagtttct aatgatgttg cttatcaaat tataataggt
     1501 actcatgttg tagatgtata taacgagttt atgtctatga taggtttaag ttcaagtgaa
     1561 tcaagtataa aaagtgaaag aaaaaaggga attagaggta tcaaaggtat atttaattcc
     1621 atctttgtgg tagtctcaga gactatgact tcagtaattg aagtactact tgcagctggt
     1681 atactcgcaa gtatattagc acttttaaat atgtctggca tacttcagag tgatagccct
     1741 acatatcaaa tattagatac attaagaagt gctacatttc acttcttacc agtattaatt
     1801 gcaatagcat cagcaaaaag acttaatgta aatccctata tagcagtagt gttagcagtt
     1861 acgttattat cgtcatcaat agatggtgta gaaggattaa gtttatttgg tataaattta
     1921 caggctacaa cttatgcaaa cagttttatt ccaatacttt taggagtatg ggcaatggga
     1981 attataattg aaggtttgaa aaaaatatta cctaaagcac tacactattt tttagttcca
     2041 gtgttatctt taataataac cctatcagtt acattaactg tatttggacc tattggtatg
     2101 tggattggtg atggattaaa ctatgcatgt atgtttttaa ttgatacatt gggtaattgg
     2161 agtgttgttg cactgtatgc agctttacaa ccactcttaa tagtttttgg tgcagcaaac
     2221 tttactatgc ctatatcttt aaattctgtg acaacattgg ggtatgaccc aatttttatg
     2281 ggcgctgcaa caatatctga tattgcagtt tgtggtgcaa tgtttggata tttcttaaaa
     2341 actaggaata aagaacaaaa acaattattt gggacagtaa gttttagtgc acttatggga
     2401 gttacagagc cttcagtgtt tggtgtgttt atgaaatata gaagaccatt tgtagcagta
     2461 atgataggtg gaggtatagg tggtttaata gcaggtttag ctcaagttaa aacatatgga
     2521 atggtttggg gattagctgc attaccaaca tatcttgttg gaggagaggc atctaacttt
     2581 acatttatga taataagtgt agtagtatca tttgtagcag caacagcagc ttcctattta
     2641 ctaggaatac cttcagatga aaaagaagaa gttaagggtg ataatgaaaa agaattagaa
     2701 ctaataatca acgagaacaa ttcaagaact attagtattg gaaatgttgc aaaaggtcag
     2761 gtaattgcac ttggggaagt taatgacaaa gcattttcct ctggagcatt aggtaaaggg
     2821 gttggagtta tacctagtga atcaaaatca actgttatat ctcctgtaga tggaactata
     2881 actatagtat ttccaactaa acatgcatat ggtatagtga ctgatgaagg attagaaata
     2941 ttaattcata ttggtataga tactgtaaac ttagatggaa aattctttga aagtcttgta
     3001 acacaaaatc aaagggttaa aaaaggagat gctttagcag tatttgaatc tgataaaatt
     3061 atagaggaag gctttgattc tactataata actgtagtta caaatactag cgattattta
     3121 gatgtaatct catcaaatga tgaatcagaa gaattattaa ctgtaatagt ataaatttta
     3181 ggagggaaaa ttcatgaata ttaaagatac atttttctgg ggaggaagta ttgcagccca
     3241 tcaatgtgaa ggttcatggg atagtgataa caaaggacct gcaattatgg attttgtaac
     3301 taagggctct tatgaaacgc caagagtaat tactgataaa atagaagaaa aattggatta
     3361 tccatctcat aatggaatag atttttataa tagatataaa gaagatatag ctttatttaa
     3421 agaaatggga tttagtgcat taagaatatc tatagattgg tcaagaattt ttccaaatgg
     3481 agatgatgaa aatcctaatg aattagggat taaatactat gaaggtctga tagatacatt
     3541 aatagaaaat aatatagaac ctattgtaac actatatcat tttgaattac ctatgaattt
     3601 agtacataaa tatggttcat ggaataacag aaaacttatg gatttgtatt taaagtacag
     3661 tgagactgta ataagaaggt ttgatgataa agtaaagtac tggattacat ttaatgagat
     3721 gaaccatatt gaccctcaaa cagagcactc ggatattttt acttatatga tagcaggtat
     3781 taagtatagt gaaatagaaa ataaggttca aacattggca aatgtagggt ataatatgac
     3841 tttggcgggt gtaaaggttg caaagttggc tagagaaatc aatcccaaca ataaagttgg
     3901 atgtgtattt ggactaaatc caatttatcc atacaattgt aatcctagta atgtgttaaa
     3961 tgcattttta gataatgatc gtgattatta tcaaattgat gcaatgtgca atgggaagtt
     4021 tccccaatat aaattaaaag aatatgaaaa aaataatata actattgaag tgactgatga
     4081 agacaaaaaa gcatttaaag aaggtaagtt agattttata ggtcttaatt attatttttc
     4141 aagtgtatct actccaaaag aaaatttatc aggtgataaa tctttatttg gtgggattca
     4201 aaatccatat ttggaacaaa gtaagtgggg atgggcaata gaccctatag gtttaagatt
     4261 taccttgaac tatatttaca gaaagtatca attacctatc ttaattacag aaaatggttt
     4321 aggggcatat gataaaatcg aagaagatgg aactataaat gatgattata gaattgaata
     4381 tttaagtaag catctatctc aaatgaagtc tgcaattgaa gaggataatg ttgaatgttt
     4441 tggatatttg atgtggggac caattgattt agtcagtgca actacaggtc agatgagtaa
     4501 gagatatgga tttatatatg ttgacttaga tgatgaaggc aaaggaagtt ttaagagata
     4561 taagaaaaaa tcatttgatt ggtacaaaga agtaataaaa tctaatggag aagttttaga
     4621 tttttctacg gatatagtag tagggtagta ataagataaa tagaagttag gaactaatgt
     4681 taattagtgt ctagtaaagc atttaaaata gagtataggg tctttaaagt aaagtgtgta
     4741 ttcttattaa aagtaatggc aactaaaatt actataaaaa ggagaataca cattttttat
     4801 gtttatactt aagtaaatat ttagacatat tcatagtata atttactcat tatataaaaa
     4861 tagtatgatt tactcactat ataactttat aaattttcta gaaaaatgtc agaatatatt
     4921 gacaaaaaaa agatttgaaa ttataatcta tattgagaat agtttgacaa tataaaaaca
     4981 agtattttta caaatagtga aattattttt attgtcaatt taagaataat ttaggactac
     5041 tataaagatg aaggtataga tgtcattata tgataaatca tacttgtata gaaagaattt
     5101 taaagtatgc ttatcattga tttaatataa taaataattt ttgtttctag taatagttca
     5161 taggaataag atatctaaat tcgcttagaa atagtctaat ttacaaagga ggggatgcac
     5221 tagtagataa aatttttata taaagaagac ttaaagtgga gtatattctg ataatattgg
     5281 tttaatggtt atattattat ttcgttttag atattttaag aataatttaa gacgtttgta
     5341 aaaatgtatt ctatatctaa tatttaaata aatataagtc ttccaacatg tatactaaga
     5401 tagaaagtag ttgaagtaca attctaactt gtaaaattat gtagaaagta aaaatcaaaa
     5461 agaaatctag taagttgata attatagtta atatttagag agttaactct agttatattt
     5521 atttagatat tttaggaata atttaagacg tatataatat catcaaaaaa tatcaagcta
     5581 taccatatgt agttaagaat aactgcataa tatacatttt ataattattg ttttattgat
     5641 aaaaaattat tatatttagg aggaaacaac aatggaaaat ttttggaatc cacttacttg
     5701 ctttactgta tgtgtaatga tttatgcttt tagtgagttt ttatctaaga aaacaaaagg
     5761 tgcaatttca acattgttat ttgtatgtat tatattttta ctaggatttt ggacaaatgt
     5821 tttaccaaaa gacattacag gagcatcagg gatattagct gcaatgagca actttggtat
     5881 agctttatta ataactaact taggaacact tataaatctt gaagacttat gtcatgaatg
     5941 gaaaacggtt gtaattgcat tagtaggtat tgtaggaata ggggttggct gtttaacagt
     6001 gggtagtatg ttatttggta gagagtatgc tttaatagca gcacctccaa tagcaggttc
     6061 tactgttgcc ggtattatcg taacaactgt tgcagaagct gcaaatagac ctgaacttgc
     6121 tgcatttgta attctagtat tatcatttca aaagttcttt ggcattccga taactacata
     6181 ttgtataaat aaagaattaa aatttaaaag agataacggg gattttttga aagatgagtc
     6241 aaaaagtaat tttagacttc caagtatgag aatttttaaa gaagatttag gtaaaaaatc
     6301 atctactcta gtgtatcttg caaagctttc atttgttgct atgattggta attttgtagg
     6361 attggctact ttaataccag gatttacccc tgcaaactat tatttaaatc ctaatatagc
     6421 atgtttgtta gtagggttga tttttacacg aataggtttt ttagaaaaag gaattttaga
     6481 taaatctcaa tctaatggaa ttataatgtt tggttgtatg ttgatgttac ctggaggact
     6541 tgctaaggta actccaacag cattgtttgg gatgattgtt ccaactatag gaatacttgt
     6601 tgtaggttct gtatttataa tattatttac tgcaattgta ggaaaactat tgggatattc
     6661 tccaagaatg tcagctgcaa ttggtgtaac ttgtatgcta gcttatcctg caacacagat
     6721 aatttccaac gaaggtattg atgctatgga aggaactgaa gaagaaaaac aaagggcaac
     6781 agattattta ttaccaaaga tgattatagg aggatttgtt actgtaacca ttgcatcagt
     6841 ggcatttgct agtataatag gtccaattat atttggttga tagtaaatat acttatagga
     6901 ggaataagaa atgataggaa gtatatctat aaatgagata gataaaaaaa gattcaaact
     6961 tgatgattta agtaagaaaa tatgggaaaa cccagaaaaa gcatttaaag aatttaaagc
     7021 atgtgaaaat acagcaaact ttctaagaag tgaaggattt gatgtggaag taggtgtagg
     7081 aggtttagcc acggctatta gagctagttt tggttcaggt aaaccagtta taggttttat
     7141 gggagaattt gatgcattac ctggtttaaa tcaaaaagtt tcaacaaagc aagaagcatt
     7201 tgaattgagt gcatatggtc atggatgtgg gcataattta ttatgtacag cacatgtagg
     7261 ggcagtagta ggacttaaaa gagagatgct agaaaataac ttaagtggga ctatagtatt
     7321 ttatgcatgt ccaggagaag aacaattaac aggtaaagga tttatggcta gaggtggagc
     7381 atttgaaggg ttagacttag ctataaattt tcatccaaat aaaattagtg aagctacaat
     7441 tggtacttca acagctgtta actctgtaaa atttcacttt aagggaaaga ctgcacatgc
     7501 aggctcagac cctcaaaatg gaagaagtgc actagatgct gtggaactta ctaatgtggg
     7561 agctaattat ttgagagaac atgttacttc tgatgtaagg attcattata caattacaga
     7621 tggaggagta gcacccaaca tagttcctga taaagcatca gtatggtatt atacaagagc
     7681 tttaagtaga gaagctgtag aaagtacata tgaaagactt gtaaaggtag caaaaggagc
     7741 tgcaatgatg actgaaactg aagttgaagt agagttttta ggaggttgtt ataatacttt
     7801 aaataatcat gttttagcaa atcttgtatc agaatgtatg aatgagatta aacaagagcc
     7861 ttggactcga gaggaattgg attttgcagc tgaattagat agacaaagcc cacaacaata
     7921 taaggctatg ataagtaaat ataatttgcc agaaggaact catctatatt atggtggagg
     7981 aaatgttaca aactttaata gttatggttc tactgatatt ggagatgtaa tgcatatagt
     8041 tccgacagca tactttttta cagggtgtac gaatctaggt gcaccaggtc atagctggca
     8101 gtttacatct tgtgcaggaa gttctatagg agaaaaaggt atgatatatg catcaaaaat
     8161 catggctatg tttggagcca aaatattaaa taatccagag atagctaaaa aggcaaaaga
     8221 agagtttgat aaatctatga atggtaaaac atataaatgt cctattccag atgatattcc
     8281 aactccttaa aaagacatga agtttaatat ataaaattat aatttttata tcagcatcat
     8341 ttcattacta aaagaaagtg agattttgct gactatctga ttaggaggaa ggaatatgaa
     8401 aaattttttt aaagaagcgc agtcaataga aggggaaatt attagatgga gaagatattt
     8461 acatgaaaat cccgaaattg gttgcaatct accgaaaaca gtaacctttg ttaccagtac
     8521 tttagaaaaa atgggatatt ctccgaaatt gacgtctaat aatggaatag tagctgtttt
     8581 agacagtgaa aatggtggaa agaccttatt attaagggca gatatggatg cacttcctat
     8641 tatggaggag acggatttac cttttaaaag taagaataaa gatgcatctc atagttgtgg
     8701 acacgataat cacacagcaa tgctcttagg agcagctaaa atacttatgg ataataagca
     8761 cttacttaaa ggtagagtaa agtttgtatt tgaaccagat gaagagagta ccagaggtgc
     8821 aaaatcaatg attgatgatg gtatattaga aaatcctcag gtcgatgcag ctatggcatt
     8881 tcacagtatg gtgggtaaat ttcttaacac aggtcaagta gcttattcac gtggtccagc
     8941 tatggcatct gcggatatat ttgaaataac agtagaagga aaagcaactc atggagcaag
     9001 ccctgaattt ggagtggacc caataaacat aatgtcgcat atacaaatag cattaaacac
     9061 tatactttca agagaaaaac ctcagaaaga gcctgtagtt ttaactattg gtatgtttaa
     9121 tgcaggaaac gtaccaaatg ctataccaaa taaagcatct atgtcaggaa ctttaagaac
     9181 atttgaccaa tttgttagag aaaaattaaa aaatagaatt attgaaatat ctacaggaat
     9241 agctaaatca tttggaggaa atgctaaagt ggaatttaaa atggggactc ccgcagttat
     9301 aaatgatttg gatgtaggag atgagattgt atcttatgtt aaagaaatta taggaaatga
     9361 gaatgtaata gactttccat ctgcaatggg ttcagaagat ttttcagaga ttttactaag
     9421 ggttccagga gtatttttat ggattggaat gggaagtatt agtcagggat ataattttgg
     9481 tatgcataat cctaaagcaa tttttaatga agagggatta gtttatggtt cagctttatt
     9541 tgctcattgt gcagtaaaat ggttagaaaa taattttagt agataataac ataaaatatt
     9601 attaaatatc ataaaaggta tatcttaaaa agatgcaaag atataccttt tagaaagtca
     9661 tatataattt tacttaatat tagatgataa aatattttta tgcattttgg attttaatat
     9721 ttattttctg atataaataa tttgtatacc ctttcaggtc taccttttgt tgagttttgt
     9781 ttttcatata atatagtagc gtttccagac tttaagagac tacttagaaa tctattagca
     9841 gttctaactg ttacatttaa acatgaagat aattccttag gagtaacttc atctctttgt
     9901 agaatttcag taacagatat aatcttttga atagtaagag tagacaattt agaacttttg
     9961 gcaattgtat ttatatgtgg agttacttta tttgaaacag taagtaactt atcacacagt
    10021 aaaggtccta ttgtctcata attttcgttg actaagaaac tgtaatttaa aggatttatt
    10081 ttagattcct tattagcact tatggcacta gacatagctt gctttatatc attaccaatt
    10141 ccatatccaa cacatacttt aatatttgta ctattttcaa tagattcttt tatataacat
    10201 gcagtaaaat ttttagttaa tgatttaatc gtttcacatt tagtgaaaat tttaaaagaa
    10261 ttactatttt tttcaattat aaaatcggtt aatttatctt ttttaatact atttaatatt
    10321 ttataaactt ggtctagagt agagtcatca taatttgtat ctttaactgt tatatatatt
    10381 acacttggtt gatttccctt cattctatgt aaatgaatat cttttattaa gttattaaaa
    10441 gtatttttta ataaatctat atcagggtat atgaaatgaa agtttactcc tgcattttgt
    10501 aacataggaa caatactact aaaacgagta gtagaaaaat cgattttccc ttcattccat
    10561 agattaatat gtttttttgc aatctttttt tcaagttttg ctatgtcaga taaactcatt
    10621 ttagatgcat tttcatcaat actatccatt aagtctccaa atgtgccatt tgataaataa
    10681 tatgatatat cttttggatt atctgcccag tctgttaagt caaagtatgc tctagaaaaa
    10741 tctaatgtat tatatttaaa gataagcttt agaaacatat catagtaact ttgtaagtca
    10801 gtaccaaact gaacctccca tgactaaagt cacagggttc ctgcttcata gaattttgca
    10861 tactaaaata tgtcttacta attcttcaca ggccatcccc gtagttccta cggttcttac
    10921 ataagttatt tattttgaat tgttattagt cttaatcctt cttttaatat atttatactt
    10981 gcatttatat ctctatcgtg agtttcatta caactaggac aaatccattc tcttatattt
    11041 aagtccttca cttcctcgtt tttataccca catttattgc atatttgtga acttgcaaag
    11101 aatttaccta cttttacaat ttttctacca tgccaattag ccttgtattc taattgacga
    11161 ataaactctg accatgatac atcagaaatt aagcgagata gtctacgatt tttaatcata
    11221 ttctttactt gtaaatcttc tatgcaaata atatcattct cttttattaa tttagtagat
    11281 aatttttgta gaaaatcatt tctttg
//
LOCUS       DAMJVX010000115        11259 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.120, whole genome shotgun sequence.
ACCESSION   DAMJVX010000115 DAMJVX010000000
VERSION     DAMJVX010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11259
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.120"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(72..497)
                     /locus_tag="PC407_002624"
                     /pseudo
     CDS             complement(72..497)
                     /locus_tag="PC407_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
     gene            complement(970..1257)
                     /locus_tag="PC407_002625"
     CDS             complement(970..1257)
                     /locus_tag="PC407_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498059.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(1277..1552)
                     /locus_tag="PC407_002626"
     CDS             complement(1277..1552)
                     /locus_tag="PC407_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498060.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAIGMLKGVDYEPLIVATQVV
                     AGTNFKFICNATSVTNPPHDYLAEIIVFKPLPCDEHNATITSINIIK"
     gene            1740..2559
                     /locus_tag="PC407_002627"
                     /pseudo
     CDS             1740..2559
                     /locus_tag="PC407_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(2818..5385)
                     /locus_tag="PC407_002628"
     CDS             complement(2818..5385)
                     /locus_tag="PC407_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2498061.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTNQLEDVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMDGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIALQSEKE
                     LWGLSEWVKQNNSINLYLQNSPAIKIAEDFMSSVMPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     APQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(5388..5993)
                     /locus_tag="PC407_002629"
     CDS             complement(5388..5993)
                     /locus_tag="PC407_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2498062.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(6367..7827)
                     /locus_tag="PC407_002630"
     CDS             complement(6367..7827)
                     /locus_tag="PC407_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2498063.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDRAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8158..8736
                     /locus_tag="PC407_002631"
     CDS             8158..8736
                     /locus_tag="PC407_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2498064.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIKRHNLKLKIEKEKAFGVCNS
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            9028..10278
                     /locus_tag="PC407_002632"
     CDS             9028..10278
                     /locus_tag="PC407_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2498065.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            10439..11008
                     /locus_tag="PC407_002633"
     CDS             10439..11008
                     /locus_tag="PC407_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2498066.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVDFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAVAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3711 a         1960 c         1583 g         4005 t
ORIGIN      
        1 gtttccctat atgctgacta ttttagctac taactcatga ctaaattcac gagtgtgcgt
       61 agctattttt taatcaatac aattactgca catatagatg cagaattttt aaatacttca
      121 cctaatgaaa ctgctgcact aatatcatgg gctactcccc tattgtcgct aaaacctgca
      181 gtatcacata attttgcatc cattccttct attggttttc tcatatttac aatgtttggg
      241 tataaagttt gagaaacagc tcctgaacca atctctgcca cagggtctaa gcaatcaata
      301 tatgatatac cagtatcttc atcttctact tccatcatta cacatcctgc aagatagtta
      361 attgcagtac ttttgccaca tcctgtccca ccaagaatta gaattaaatt ttgattttca
      421 gccttattta ttgctacttt tccttcattt aataagagtg ttatttcttc taaaagttga
      481 ttgttatttt ctggcattta ttttccccct tggttatata ttttatttaa ataaagttta
      541 atcccttaag tagattacaa agtttttaga ctatatttaa atttgttttt tatttttaat
      601 aaatttgcat tttcactcaa taattattct tctttgttta ttaaaataac tgtgttgtat
      661 aatataattt ttaccaacac agttatttta tttaatgcgc ttattttatt gtattttatg
      721 taatacagtt gtttttttat attatatgtc cactattttc ctatggttat tacatataga
      781 tttttaatta tatttgtacc attaatttaa acattcctat actactgctt aagttttaca
      841 cctataccag cttgaatatt ttatttttat ttgcttaaat agttcaacta tactggttta
      901 ataggtttcc tttagtttaa atagttcacc tatacctact tgaatagttc acttttattt
      961 gcttaaattc tataccaata ctggcttaaa cattatacta ccatgattgt attgtctatc
     1021 ttcaactaca ataaaaacag gttctaaatc tttagtagat gttattttta attctaaaaa
     1081 aacattatca gttggtaata tttttacatc ataattattt aaattattat tcgtgatact
     1141 attaaataca tcaccttgac gttcaaaaaa aacattatca actattacaa aataaacatc
     1201 ttcttttcta ctattttcaa cctctatagt attttgttta gttacattta gtctcatctt
     1261 ccacctccta ttaatattac ttaattatat taatacttgt aattgttgca ttatgttcat
     1321 cacaaggaag aggtttaaaa acaattatct ctgctaaata atcatgtgga ggatttgtaa
     1381 ctgaagtagc attgcaaata aatttaaaat ttgttcctgc tacaacttga gtagcaacaa
     1441 taagtggttc ataatcaacc ccttttagca ttcctatagc ttcattaaat acttttaaat
     1501 cttcttcttc gacagcccta aattttgacc atccaccaac aaaaagactc atataaaaac
     1561 ctcctttata tatatattta tttatatgat agtaccaatt ttcttaatat tcaatatatt
     1621 acgtctaact ggtcaataaa aatgcataac tggataaaaa gacttataaa atagcttaac
     1681 tttacatata ataagattat tctaaaatat gagacatatc ttgctttgaa tatattctat
     1741 acaacaattt tccaggcctc attgccaagt ttaaggtaac tgactttgga ttccaacttc
     1801 ttgatgctaa taaaaaatat tttgatatgt tcaaagataa tgtaaatttt ctattagatg
     1861 atataaattt agaaggttat cttaacaatg tgtataaata tcataatgat tttaaatctg
     1921 ggagatgcgt ttgtttttct atttctccac aaactattaa tggagatatt tttcacatgc
     1981 aagttgtagc tgaatgtata gattgggaag atgataagcc tatatatctt ttaatatatt
     2041 cagatgtaac gacattggta aaacaatatg tctcattatc atttttcatc caagaatttt
     2101 atcaagaaat atgataaaat agctaaaaaa tatgatattc cttctcacct tatagaaatt
     2161 gagattacag aaacaattgt atttgaagat gtcaaagtat ttagtgaaat aataaatgaa
     2221 attcataaaa atgactttaa atgctctatt gatgattttg gaagtggcta ttcttcaatc
     2281 aatatgctta agggtattta tgttgataca ttaaaattag atagagcatt ttttcaatca
     2341 gaagatttag attctcaacg tgaaagaaat ataattgtat ctataatcga actagctaaa
     2401 aagttaaaaa tgaccactgt agctgagaga atagagacca gttttcaaaa agatttcttg
     2461 caagaaataa actgtgatat gttacaagga tacgtgttct ctagaccttt gcaaataaaa
     2521 gactttgaaa aacttatgtt tcaaaactcc tccaattaac aatataaaat tgcttattat
     2581 aagtgtatta ttgtatcaac aaataaagga attgtctcaa aattgatttt ttctaatcaa
     2641 atttttattt tgagacattc tcattttttt gcataagaaa atacccacca agctacggtt
     2701 tttcttaccg ctgttcggtg ggtattagta gtattatgaa atctgaaaac tttaagtatt
     2761 actacttccg ctaacgcaat cacaaaggtc gaaagcttca aagcgaaaat aggcttttta
     2821 ctcatcaaac gtcaccgtac ctgccaaccc gtccaccgtt atcatttgtc cgctcttaat
     2881 agttcgtgtg gcaatgtgtg tagccataac ggctgggatg ccatattcac gtgcgacaat
     2941 gcttgaatgg ctaagaggac caccaatgtc cgttacaatc gcacttgcag atgaaaataa
     3001 tggtgtccat gctggagttg tggtaactgc aaccaaaaca cttccaggtt gaaagctttc
     3061 aaaatctgct ggactattca gtacacaagc tggagccgtc acaacaccag tactggtacc
     3121 aatgcccgtc aacacaattt tcccatcttt ctgcttctgt ggtgcttgtg atatagtttt
     3181 tttgtttttc tctggcaact tggacggtga cacataaccc atgtattttt taagttcagc
     3241 ttttcgaaca ggtattatcc cgttcatatc agatagagac ttgtttttat ccaactgctc
     3301 tataagtacc tccaattccg attttgtgag ccaataaata tcatctaaat gtgaaatagc
     3361 tcctcctcgt atgaatcgtt ctgagatttc ctgaaacata caacgaatca gaggatgtcc
     3421 cattcccatc aaataaatag cattttcacg cataggagag gtttcctgtg cccaatggag
     3481 caacttaaaa aatagttttt tacgcaatcc acctatgtgt tgtaaaatct cttcttctgc
     3541 atgttttcta cgcttttcaa acgtggtctg gcgtaaaaaa ggactttccc cttttccttc
     3601 cacaaatgtt tttacggatt caaaagttgg tgtaagtgtt tcctgcggtg tggaatacgc
     3661 aaaatcaaac tcataagcgg tacgaccaaa ctctttaaag tacttattga ttcgattttt
     3721 ccattctatc catacttcct gtgaaactcc aacaggcata actgaagaca tgaaatcttc
     3781 tgctatcttt attgcaggac tgttttgcag ataaaggttg atgctgttat tttgctttac
     3841 ccattctgaa agaccccaca actccttttc cgattgcaga gcaatcgtat caaacccaag
     3901 caggaaaacg gaagtatccg ttataccagc acgtcgaact gctccttgaa ataattttgt
     3961 aaataatgtt tcactgatag aagcagcagg caatgtaagc tgaatccttg tgaaataaat
     4021 acatgcggca taaaatattg tctgaatacc atccataatt tgatgggcgt tcatcgtatg
     4081 caggggcttt tcttcccatt gtttaatagc atcctcatat ccttttcgtg ccgtttccca
     4141 gcgtgcaacg ctgttcataa gtgtcctacg taaagagcgt ggggaaaggg atttgatagc
     4201 aatcaaaagt ggctttgatt tagcagaaag ataaacatat ccattaataa ccgtatatgc
     4261 gccgtttttt ggtaataatt ttttcgcact tttaccaaac atatctatcc ataaaagctc
     4321 cgatgcacgg tttacgattt caagaccaag cgtggcgaac aaaggtgtaa caggatttgg
     4381 caagtgttct gccaaacttc cctttgtgta tacaacatcc tgttctggca atacccattc
     4441 cggtggcaga acagtaattg gacgagcttg aacaatgtac aatttatctt tttccagtgc
     4501 ccactctaca tccataggca tttgataata cttctcaatt ttctttccaa gctgtatcag
     4561 ttgcattacc tgattacgag taagagcata tttttttctc aacctttcag gaaccatggt
     4621 ttcttctgtt ccttctgaag tacgaaccgt cataatctct ttatttgcca cttcatacga
     4681 tataattcgt tcggagtcct tatccactac gattgtatcg ggtgtaacta agctagaaac
     4741 caccgcttca ccaagccccc atgcagcgtt aataatcatt tcgctacgtc taccattgat
     4801 tgggtttaac gtaaacataa taccagatga atcggaaaat gcaagctttt gtactacaac
     4861 agcaagtgcg acaatctcgt gctttatatt attcttcaca cgataagcaa tagcacgagc
     4921 tgtccataag gaagcccagc atctctttac agcgtcaata actttatctt caccttgtat
     4981 gttaagataa gtttcctgct gacctgcaaa agaagcgtca ggtaaatctt cagcagtcgc
     5041 agacgaacga acagccaccg atatgttccc taatccggca taagctgttt taattgcgtc
     5101 cgacacttcc tgtggcattt ctccattatg gaaaagctcc cctattttct tagatacatc
     5161 ttcaagttgg ttggtattat tggaatcaat accatctagc agtttattaa ttcgagactg
     5221 aatatggttc gtctcaacaa aaatctggta tgaagcagtc gtaacatgaa aaccgtctgg
     5281 cacagggatg cctgctgtca atagttttga taaagacatc ccttttccac caacaatttc
     5341 aatggttgct tgtttatgtt ccagtggcaa gacataataa atcataatca ttcacctcca
     5401 tcattcataa ttccgtgcag aaatatattc attgtttcat ccaacatttt ttccgaacta
     5461 tccgatggtc gtgacgtgta gctgattgat aacatggagt taattaacaa acgtgtcaca
     5521 agagcggtat ccgttttgcg aaagatgcct gtggtcacac cattatcaat taccgatttt
     5581 ataatatctt gatatgcata acgagcatct ttaagtctct ttcgatactc ttcttctaaa
     5641 taatcagatt ctgtatttaa ccacatcagt acagttctat actgctctgg aaatctaaga
     5701 tgattaagca tgatttttct aaggcactgt tgtggtgaag gttcaccatc aataatccta
     5761 tgaacttgtt ggatagtttt ttctatttct ttctcaactg catatacaac aatctcgtct
     5821 ttcgttttga aaaaatcgta tagacttgac tttcccatgt tggctaaaac tgcaatttct
     5881 ctcattgatg ttttttggaa tccattttca gccatgagac tcaaagcaac agcaactatt
     5941 ttttctcggc gttctgcttg ttgttcaggt gtaagcacaa ttccttttgg catagggcga
     6001 tacctccttt tagcgaccaa tggtcgtttc ttttttattt atcatacagc gaccatcggt
     6061 cacttgtcaa ggttaatttt tttcattgta tatggtactc gagataaaaa tgttcatttt
     6121 agaaattgct ctatttaagt agaaataggc ttcatcatta gaaaacaaga ctataaattt
     6181 gagatattta tcatgttcaa atttcatcaa tatcaaagtt taagatatta ataaaaaaag
     6241 aattactaag aaataacata taaagattca aaaaaggagt tgaacttaaa attcaattac
     6301 aaaaaatcaa atttaagaca actcctttat tctttatctc tactattctt tatctctatt
     6361 tatttattac aactccctaa catttaccac tactgcctta ggtctagtca tagcctcgat
     6421 tgaatactta acaccttgag ttcccatacc agatgccttt acacctaaaa atgggaagtg
     6481 gtctggacct ctttcagtct tattatttat ttgaacagta ccaacttcaa gtttatcagc
     6541 tatataaaat gccctatcaa tatcacttgt aaatactgat gattgaagcc catactctga
     6601 ttgattagca atttcaattg cttgatttat atctttaact cttataattg gaagtactgg
     6661 accaaatggt tcttcccaag caattttcat atcaacattt acattatcta acaatgttgg
     6721 atagaataaa ttattttttc taacattacc tgttattaac tttgctcctt tatgtaatgc
     6781 atcatcaacc aacccttgaa caaaatcagt tgctttatta tctataagag gagtgataac
     6841 tacttcatcc attggatttc caattgtcaa cttctctacc aatggcttta tttttccgac
     6901 taatttatcc gccacactct cttgtactaa aattctcttt actgccgtac atctttgtcc
     6961 tgaatatgaa tacgctccag atactatatt cttagctgca aaatctaagt ctgcatcttc
     7021 taatactata gccgcatctt ttccaccaag ctccaataat agtggtacca ttccagatat
     7081 tttagatata tgctgaccaa cttcagtact accagtaaag ttaataaaat taattccttt
     7141 atgcgttaca acataatccc ctatctcact acccctacct gtaatagtat taagtactcc
     7201 atctggtatc ccagcttttc tcatgatttc taccacatga agtgcgctaa tagccccttg
     7261 tgtagcaggt tttaatacaa ctgcatttcc accaataagc gctggagcaa tctttgacat
     7321 tgataaattt acaggataat taaaaggaga tatagctaaa acagtaccta atggaaccct
     7381 tgaaacataa gacatcttat ttcttgttcc accagggaaa ttatctccac taatggcttc
     7441 accctccata ttttttccca catcagctgt gtatcttaaa aaatctgctg ttctctcaac
     7501 ttctgccctt gcagatttta tgtctttggc tatttcaagt accagtatat tagctatctc
     7561 atctatattt tctaacaata aatcagcagc cttatgaaat atattggctc tcttgtatac
     7621 aggcatttca gcccatactt ttatactctc ttttgtgttt tttataacct catctacctc
     7681 atgttttgtc atagattgta ctttcccaac aagagaatta tctattggtg agtaaatttc
     7741 tattggcttt ctcgaattag attctaccca ttttccattt attaaatttt tatatacatt
     7801 ttcttttgtt ttaagttcat taaacatatt tttattcctc tctctgatat aactttgtgt
     7861 atttaaatta tagccattta ttcgttatgt aaacatttct ttttttcata aacgtttacc
     7921 tataaaaaaa attttaagtt aacgtttttc tagattcttc tattttaata acatactcgc
     7981 tccaaatcta tataggtcac actttaatat caatatacca atataccaat ataccaatat
     8041 aaatattata aacataaatc acttgacaaa tttataagta gacaatatta tatatctatg
     8101 acttaaaata tttttaaagt catagatagc atatgcagat agaaaggatt gataaaaatg
     8161 gctaaaagtt ttacagaaat agaaaaagac aatataagaa aaagactaat ttcagaatgt
     8221 gaaatcaact ggtctaaata tggatacaaa aaaactaaca ttgatgaact ttgttcaaaa
     8281 tcaggaatcg caaagggttc tttctataca ttttttgatt caaaagaaaa gttatttttt
     8341 gaagtactaa atggaatcca aaacagactt atatcatcag tagatgaaat tttaagtaac
     8401 atgcctccaa aagaaggctt tgcaaaatcc ttaaaaatgt tatatcgatt atatgataaa
     8461 aatccatttt tatattcacc aaataatcct gatttcatca gcctaataaa taaactacct
     8521 caagatatgt ttttacaact tgagtccaat aacatacaaa gtttttacga cttaattaag
     8581 agacacaatc tcaagttgaa gatagaaaaa gaaaaagcct ttggtgtttg taattctcta
     8641 ctatcaagtc tattggtaaa agaaaatatt gaatataact atattgaagt atttgatttt
     8701 atggtagata atcttgtaga acacatactt gaataacaat ttaaaataat ttttctaaat
     8761 atttctttat actaaagagg attaatatga ttgaattaat gaaaaaaata aacaatatca
     8821 tttacaaaat aaatgataaa ctaaatatac acacaaaact tatagacccc agagttaact
     8881 cattccactc tactctaagc catctataat agttcgtata tttctaataa tattagatat
     8941 taaagtaaga aaaaaattcc ttataataat tgctattata tcaattatta taaatatttt
     9001 aataatctta gataggtagg tatatatatg gtaaaatcct caaacacttt taataaaaac
     9061 ttcaacttga tgatattagg tcaaataatc tcattgtttg gtgcatcaat acttaaattt
     9121 gctttatctt tatacatact tgatataact ggtaaagcag aaatatttgc tactatacta
     9181 gcagtatctt caatcccaat tatagtattc tcccctattg gtggagctat tgcggatata
     9241 ttcaacagaa gaaacttaat ggttattttt gatttttcaa gcagtcttac agtactaatc
     9301 ttagcagtat tcttattcaa caataatggt tcaatattac ttgtaggaat tattatgacc
     9361 atattatcag ttataagcac aatgtatcaa ccaactgttc agtcaagtac acccctttta
     9421 gttgataatg agcatttaat gaatgcaaat ggtattgtag ctggagttgc atctcttaca
     9481 aatattgcag gacctgtatt aggaggtgtc ttgtatggtg tgattggaat taatgcaatt
     9541 atagttataa gttgtgtaag tttctttcta tctgcaatta tggagatatt tatacagatt
     9601 ccttttatca aacaagagca aaacagcaac attataaaaa ctatatttac tgatataaag
     9661 gatggtattg ttcatatatg taaagaaaat agatttatac taaaaatact ttttttagct
     9721 atagcaataa acttattttt aagctctatg ttaatagttg gtatccccta tatagtaaaa
     9781 atcactatgg gagctagtaa cttcttatat ggtctagcag aaggagcaat ttcttttagt
     9841 tcactggtag ctgctgttag tattggtatt ttctcaaaac atctaaaaat cactaatttg
     9901 cacatttcct ttattatatg tgcagttatg ttcataccaa tggcggtttc agtatatcca
     9961 tttatattaa acacaggatt tccagttcca tttttaatct ttatagtatc atctatgcta
    10021 atatttttta caacgactct tatctcaata tatgttataa cagtcacaca attaaaaact
    10081 ccaaatgaac tcttaggaaa agtaatgtca attctttttg caggaacagc aattgcaatg
    10141 cctcttggtc aggttatgta tggtaaactt tttgatttat tatcagatag agtgtacatc
    10201 attatactag gagttggatt tattacacta cttatagcat ttgtatcaaa gataacattg
    10261 aggaacgaaa atctttagat acaaagaatt tagatataaa gatttaattt tcactaattt
    10321 ctgaatttaa atgcaaaatt gcgtatcagt atgttgacgt tttagaaatt gattgatata
    10381 atcaatggaa aataaacttt ataaaaattc aaaatattca gctagaggag tgataaaaat
    10441 gagttacatg ccaactttag aacaagcatg ggaaatatta agaaaataca ataaggaaga
    10501 atttcatata agacatgctc aaattgtatc tggagttatg agatactatg caaaggagta
    10561 cgaccctgag agagtagact tttgggaaat agttggactt ttacatgatt tagattttga
    10621 aatgtatcct gatgaacatt gtgttaaaca aagagaatta atgacagagc ttgatttaga
    10681 taaatcaata atcgattcta caataagtca tggatatgga ttaactggtt cagatgttaa
    10741 accagagcaa tttatggaga aagtattatt tgctgtggat gaacttactg gtttaattgg
    10801 tgctgttgca ataatgcgtc cttcaaagag cgtttctgat ttagaattaa aatctgttaa
    10861 gaagaaattt aaagataaat catttgctgc tggctgttct cgagacgtta taagaagtgg
    10921 tgcagaaatg cttggatggg aacttgataa gcttatccaa acaactatag aagctatgag
    10981 aagcttgatt ccagagatgg caatatagct cctcatacat ataccataga agttacatct
    11041 ttaaagagca tttctctttt tgatgcaact tctttttata ttttgtagaa taaaaagttt
    11101 aatattttaa actcactata taatctataa taaatactat aaataatata atgtataata
    11161 gtcataaact ttctaacctt aaaagtttct aatactcaat aaacaattct aatatacaag
    11221 tggcattata tgatttaatt atatatcttc aatttatat
//
LOCUS       DAMJVX010000116        11247 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMJVX010000116 DAMJVX010000000
VERSION     DAMJVX010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11247
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.121"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(64..891)
                     /locus_tag="PC407_002634"
     CDS             complement(64..891)
                     /locus_tag="PC407_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2498067.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(939..2375)
                     /locus_tag="PC407_002635"
     CDS             complement(939..2375)
                     /locus_tag="PC407_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HDF2498068.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(2462..3331)
                     /locus_tag="PC407_002636"
     CDS             complement(2462..3331)
                     /locus_tag="PC407_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2498069.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGNYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(3489..4142)
                     /gene="phoU"
                     /locus_tag="PC407_002637"
     CDS             complement(3489..4142)
                     /gene="phoU"
                     /locus_tag="PC407_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDF2498070.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(4170..4934)
                     /gene="pstB"
                     /locus_tag="PC407_002638"
     CDS             complement(4170..4934)
                     /gene="pstB"
                     /locus_tag="PC407_002638"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDF2498071.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(4953..5777)
                     /gene="pstA"
                     /locus_tag="PC407_002639"
     CDS             complement(4953..5777)
                     /gene="pstA"
                     /locus_tag="PC407_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDF2498072.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVIIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(5779..6696)
                     /gene="pstC"
                     /locus_tag="PC407_002640"
     CDS             complement(5779..6696)
                     /gene="pstC"
                     /locus_tag="PC407_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDF2498073.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(7078..8313)
                     /locus_tag="PC407_002641"
     CDS             complement(7078..8313)
                     /locus_tag="PC407_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2498074.1"
                     /translation="MRIFYLEIKRVLHSRRTLILLLLALVLSALMAFLPITYEGINYL
                     DNNGKVVELSGLDAIKYKKTIYANNSEISSGVVTPKKLKHALNIWKKTEEKYGKVGSE
                     DFPLDIYSKSILPIRPLLNRLPEAFANSETGFATELSEINTNDLDSFYEQCKTHLNDI
                     MKIERQTSSAQMKAQELYRKVDTPFEIYPGYSKDAFDYIIFYIFILLIICVAITAPTF
                     SGEYQTQSDSILRCTKHGHTRLAFTKILALFSIFIVMFVVCISIHLAISDLAFGTECL
                     KTSFQMLFSVISLVNINLLQAQILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAF
                     SICLIPMGIGVSWIGYLLPPGGIGLKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVI
                     EIPIFIFLAIRTYCKHQVS"
     gene            complement(8314..9201)
                     /locus_tag="PC407_002642"
     CDS             complement(8314..9201)
                     /locus_tag="PC407_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498075.1"
                     /translation="MELKIEHVTKKFKDKIAVEDVCLTLTPGVWGFLGANGAGKTTLM
                     RIITDIMKPTNGKILYNGKNIYIMGAEYRNLLGFLPQEFGFPQDFSVKDYLEYIAALK
                     DVPPREVKGKINNLLEILTLSDVKNKKIIKLSGGMKRRVGIAQAMLNDPKILIMDEPT
                     AGLDPGERVRFRNFISEFSHNRVVLISTHIVSDIEYISTQNAIMKSGKILDVGTTDKL
                     VKKVEGKVWECLISNRDISKYEMNLRIINQRSEKNNQVSIRYLAEKSQIDNSIAVEPR
                     LEDLYLWLFPEAVYSKEGK"
     gene            complement(9346..10461)
                     /locus_tag="PC407_002643"
     CDS             complement(9346..10461)
                     /locus_tag="PC407_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2498076.1"
                     /translation="MKKINNFTYRKLRDKYKFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKPWNYLREIIN
                     ATHTIYQQNDELVELSKPLKEIEQQMNQIKMSVLLSQQAVKEAENKKNNLVMYLAHDI
                     RTPLTSVIGYLSLLKEIPNMDNSQKSKYTEVALDKAQYLEKLINELFEITRYNSNQIK
                     INFEKVDLYYMLLQLKDEFYPLLSEKGNRAILKANESLTIKADSEKLARVFNNILKNA
                     IAYSYPNTEIIISAVHKDSKVDIAFRNYGQTIPEEQLSAIFEKFNRLDEARKSDMGGA
                     GLGLSIAKEIINLHGGDITVQSKDEVTTFTISLPTSI"
     gene            complement(10497..11195)
                     /gene="vanR"
                     /locus_tag="PC407_002644"
     CDS             complement(10497..11195)
                     /gene="vanR"
                     /locus_tag="PC407_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HDF2498077.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
BASE COUNT         3601 a         2032 c         1259 g         4355 t
ORIGIN      
        1 tttttatatt atatgtttta ttttataaat aatttacttt atcttttact atatttaaat
       61 ttattataag ttttcttgcc agaaattttt aggtgttata ttttctgtaa ttggcaatat
      121 tgtatatcct ctctctttta agattcttat aatattattt aaagcattta aactttgctc
      181 tttctcatga attaatacta ctaattcatc tctttttctt ccataatata atatattact
      241 tactatctga cttgtagaag acttccaatc ctgtgtatcc aaattccagt cccaaattaa
      301 ataatcattt gccataagtt tattatatga accttctggc atatatggtt tacttccgta
      361 tggtaatctt attaattttg aactctgacc agttatttct tgtagagtat tattgcatgt
      421 attaaattcc ccaactgtta cttctgggct tttatataac ttatgtatat catgacttac
      481 actatgaaaa cctattgagt tttcctcttc caagactctc cttacttctt ccttgtgatt
      541 tttcatattt tgattaagca taaaaaatgt cgctttgaca tcattttctt ttaaaatatc
      601 aagtagtgca tttgtatatt ttgatggacc atcatctatt gttatgtatg ctactttttc
      661 agaagtagcc cctggacctt ttgtttcttc ttgagatgct ttgtatatga tttctcctaa
      721 agtattactt tgagaaagca gttccagtgt attttcacct tttgaactta tattttcaac
      781 tggaacatta ttaatttttt cctccaataa tgttccagtt aaaattaaaa cagctataag
      841 aaatatcctt ataaatacat gaaaaacagc cttattatct ttaattttca tcccccatat
      901 ctccctttta taaaagcata agtttaagac ttaaaaagtt attttttata tttattctct
      961 ttcttattac tttttatatc tctactaaat atttttttaa ccttttcttc ttctttttct
     1021 cctaaagatt ctactctttt gattcccaaa gaagttaaaa attgtaatac atttaaatta
     1081 tcaaatggag tttccatatt cgtactttca ctatattcat caacattctt agtaccttct
     1141 tccacactta aaatacgctt cggacctcct acacagcctc ctacacaccc cattccttct
     1201 ataaatgtag cttctatttc tttatttaga actttcttta gaccgtccat gcactctttt
     1261 gttccttcaa atgccttttc ttcaaatttt atatttttgt caattcttct gactgataac
     1321 ttaactgctt tacttacacc tcctgttcta gcataaactc tacctgaaag ggacgcctct
     1381 actctattac tctcatccat tattgctgga tttaactcta atgcatcaaa tatttcttgc
     1441 aactctttaa atgtaagcac aaaatcaact gcatctttta tatcttctaa cattgcttct
     1501 ttcttttttg ccatacaagg acctatgaat acaactttag catctggatt taaaattttt
     1561 acagctctcc cacaagcaat cataggagaa acggatggtg atacattctc caatatctga
     1621 ggaaaactat tttgtatcaa actaacccaa ataggacagc aacaactagt tataaagcat
     1681 ccttcttcgt cctttttcat atgctcataa taatcatatg cttcttttgc agtaagcata
     1741 tctgctgcca aagcaacttc tatcatatct tcaaatccta tcttttttaa tgcagttctt
     1801 aatttaccag ggcttacatc tttcccaaac tgtcctacaa aagctggtgc aactattgca
     1861 tatacagggc tctttatatc ttttaacaat tttatcattg gtataaattc tattttatct
     1921 gaaattgcac ccaacttaca tcttgataca caatcaccac aagcactaca tacatcttcc
     1981 tcattacaat taatatgctt acttcttatc ttacatgact catcttcctt attattttca
     2041 tcacaatatt tgcaagcatc tgatattaca gatattattg gtcttttgac ttcatctaaa
     2101 tttacagctt catgtagtga ctcttttaaa tttttatctg tatcttctgt cacatccaat
     2161 cccatagtta ccctaattaa atttaccata gtatctttat caaattttcc actaaaataa
     2221 ttatttaaat tatcctttaa atcttcattt aaaattttat taggtaagtt ttcaatatcc
     2281 tctatatcat cattccacac tgcttttaca agttcgccaa aaagcttcat tttatatatt
     2341 tctagtattt tagaattgtc atttctagta ttcataaaaa atagtacctc cataaattaa
     2401 acttatatat gtttaatatt tacaaagata ctatttttta tttattattt aactactatt
     2461 attaatatga tttatttagc tttctcaaca actctatttc aatcattatt ttcattagat
     2521 gatagttgaa tacggggctt ttttcttcat ctatattcca gtctagctta tccacattat
     2581 atgttttctt aaggtattta tatgtatgct ctttcaaata aagtttattt tcaagtaact
     2641 ctatagactg catatgaaaa tgtatttctc tacatatcct taaggcattc tcaatttttt
     2701 ctatattagt ttcactcaaa ctataatcca actcatcaat aagttttgaa tatatttctt
     2761 ctaatttttc tacttcttct tcaaatttct ctacatttaa attttctttt ctaagaattt
     2821 tatcttcaat aagtaacatt gaaagttctt ctatcttttt aaattcatct ataacactat
     2881 ttagataatt tggtctagcc acataataat taacaagtac tccaaacaaa acacctacag
     2941 atgtatctaa cactctatgt aaagaataat acacagggtc actatttcct acacctagat
     3001 gtatagccag aaaggttaca caggcaacta ctactgactt tattttaaac agattgcata
     3061 aatatattga gcacattact cctagaccac ataaaaaagg gtctccagct ttaatgatgg
     3121 caaatatgaa tcctattata ccacctaaaa tagtaccttc cactctattg attcctgtct
     3181 ttaaagaacc ttttaccgta tcttgaacag aaattataca tgctactaga gaataaaaca
     3241 tattctctac tagatagtta ccaagaatca tacataaagt gacactaatt cctgttttta
     3301 tagtcctcat acccatcttt tgaagtttca aagttccacc tcctaaaaag caccttatat
     3361 aaaatacgca tcctcatatt taatatgttc ctagtatatc acaatttttt aaatttaccc
     3421 attaatcata tttttttatc aattctttct taatttagac aataaaaaaa ctgctttaaa
     3481 gcagtttttt atcctatctc aaccatttta ccctcaattg caaaaattat cttctcacat
     3541 acatttgtta tatggtctcc aattctttca aggtatcttc ctataagtag aagttttact
     3601 ccttggtcta tagtatttga attttcatgc attattttta aacaatcaat ttgtacttgt
     3661 tcatacaatc catctacaat atcatcttta gttccaacat cataagctat acttgcatca
     3721 aataagatta aagcttctct agtctgagac atcatagact tacattcttt tgccatctta
     3781 ggaatatcta tcagctcttt tatataagcc tctccaccta tcttcaacat ttctctagct
     3841 atatttgctg cataatctcc tattctttcc aactctatcg atatattacc taatgaataa
     3901 ataaatctta agtcccttgc cattggttgc tttaatacta ttagctctat actcttatct
     3961 cttatttctt ctcttaattt atttatttct tcatcatctt ctactacttt tcttgcaccc
     4021 tctaaatcct tctctatcat acaagtcaca gacttttcta ttgcttcttc acatttttcc
     4081 atcataagta ctgtttgttc ttttaaagta tcaatactta tatctaaact attattgacc
     4141 attgtctaac tcctttatag ttatatattt taaccaaatc tacctgttat atagtcttct
     4201 gttcttttat ctacaggagt ggtaaacata gtttttgtat cgctaaattc tataacctct
     4261 ccatttaaga aaaaagctgt ttcatctgat atacgagcag cttgttgcat attatgtgta
     4321 actatgacta ttgtgtagtc tttttttaaa tcctctataa gttcctctac ttttaatgtt
     4381 gaaattgggt ctaaagcaga tgtaggttca tccataagaa taacttctgg tctcatagca
     4441 attgctcttg ctatacatat acgctgttgt tgaccaccag aaagtcctag tgcagatgat
     4501 ttaagcctgt ctttaacctc atcccaaata gctgcacctt ttaagctttc ttctacaatt
     4561 ttatctaatt gcttcttatc tcttagtcca tgtgtacgag gaccatatgc cacattatca
     4621 tatatactca taggaaatgg atttggtttt tgaaatacca ttcctacttt agtacgaaga
     4681 tttataacat catctgaggt atatatgtcc tctccatcaa cagaaatgtt tcctttaatt
     4741 gttacatctt cgattaagtc attcattcta tttaacgttc ttataaaagt tgattttccg
     4801 catccagatg gacctattaa agcagtaacc ttattctctt ttatatccat atttattttc
     4861 tttaatgctt gcttatcacc ataaaataaa tccaaatctt ttacactcat ttttatctta
     4921 tcgattaatt ccattattca cacctcttaa atttaataat tagctttatt taatttctta
     4981 gcaacaaatc ttgctagcat gtttagtgct aatacaatta ctattaaaac aattcctata
     5041 ctggctgctg ttgctatatc tccactctct ttagtcagta agtatgagtg tacagttaat
     5101 gttcttgcac tatcaaatat tccacctggc atcttggcta cagtacctgc tgtaagtaat
     5161 attgcagctg attctccaat tatacgacca actgataaga ttactccaga taagattcct
     5221 ggtattgcac ttggaagtac taccttatat aaagtttgaa attttgtagc tcctaatgcc
     5281 aatgatgatt ctctataagc ttgaggaact gtttttaaag cttcttctgt tgtacgaatt
     5341 attactggta atattattat tgcaactgta agtgaacctg caactatgga atatcccatt
     5401 tttaatgtaa ctacaaagaa aattcctcca aataatccat atactataga tggtatccct
     5461 gataaacttt cagttgcaaa tcttatagct gtaactgctt ttcctttttt agcatattct
     5521 tgtaaatata tagcagccaa tattcctata ggagttgcta caactataga aactactact
     5581 gtatataatg ttgtaattat cattggaagt ataccaccat cacctgatgc tgaataattg
     5641 ctaaataaga aattaaaatt catgttgcca attcccttta taaatataaa tcccactata
     5701 atgactaatg atactattgt aaatagtgct gatagatata ctaatgactt caatagattc
     5761 tctttgaact ttctcatctt aatttaccgc cttatttgaa agtttattta aaactaagtt
     5821 taaaattaat ataaatgtaa acaatactac tccagttgca aataacattt cttgatgtgt
     5881 accaaatgcg tatcccattt caagtgctat attagttgta agtggtctta cactatctgt
     5941 tattgcagtt ggcataactg gtgagtttcc tgcaacaagt attactgcca ttgtttcacc
     6001 taaagctctt cctactccta atactactgc agctaatact ccagatttcg cagcaggtag
     6061 tacaactttg aatattgttt ctatttttga tgcaccaagt gctaatgaac cttctttgta
     6121 tgcttttggt actgctctta ttgctgtttc tgatacagat attattgttg gtaacatcat
     6181 tactgctaat actattacta ttgctaacaa actttgacct tttggaagat taaatatact
     6241 ttgtatagtt ggaactatca ctgcaagacc aaatacacca tataatactg aaggtatacc
     6301 tgctaaaagt tcaactgctg gtgacattat ttttgctaat cttttaggtg ctacttctgc
     6361 tataaataca gaggttaata taccaacagg tactcctatc acaagtgctc caagtgttgc
     6421 aaatattgat gctactatca tagatgatat tccaaattta ttggcagaag gaacccaatc
     6481 tgcaccagtt aaaaagtcta taaatgaata tccttcaaat ataaatggtc ttaagccttt
     6541 ataaaatacg aatcctatta tcaaaagtaa acttgcaact gctactaaag cactaagtag
     6601 aaaaatattt tttgctactt tttcaattac gtattttgtc ttattgccat tattgtcatt
     6661 tttttctaaa cctactaatc tagattttac tatcataata ccctcccatt aaaatatact
     6721 tatcgatttt cgttctgtat tcaaaattta attttttata attcgtcttc atcaaatttc
     6781 ctgtactttt attctacttt ttttaagtta aatctatatt aggattaagt taagcaagtg
     6841 taaaattata tttacttttt taacattaat tttactttta aaactatatt tgacatttac
     6901 tttacaatat actgttgttt ctctatatta aaatagcaga tatagataat aatacagttt
     6961 atgtataatt tattacctat atctgctatt aagatttgtt aactaaattt aacttttata
     7021 ctacatatat aattatcgta tcctctagtt tatatcacta ttatttctat caaattgtta
     7081 tgatacttga tgtttgcaat aggtacgaat agccaaaaat ataaaaatag gtatttctat
     7141 cactgagaaa aacagtataa tgtgtggtgt ccaaaaactc atacctccta agtgtaagaa
     7201 attaaatgat gtcatttgat ataataaact aatcttaaga ccaattcccc ctggtggtaa
     7261 taggtatcca atccaactaa cacctattcc cataggaatt aagcaaatag aaaaagcaat
     7321 cagcatagaa gtaagagaat ccttacattt tgcagataaa aataatgtta aactaaccat
     7381 tgcaagtatt gaaattagac caccaatagc tataagaatc tgtgcttgta gtaaatttat
     7441 atttactaga ctaatgacag aaaacaacat ttgaaatgag gttttcaaac attcagtacc
     7501 aaaagctaaa tcagaaattg ccaaatggat agaaatacat acaacaaaca ttactataaa
     7561 tatagaaaat agagctaata ttttagtaaa cgctagacga gtatgtccat gtttcgtaca
     7621 acgaaggatg ctatctgatt gtgtctgata ctcaccagaa aaagtaggtg cagtaattgc
     7681 aacacatata attaataata taaaaatgta aaatattata taatcgaaag catctttaga
     7741 ataacctggg tatatctcaa atggagtatc tacttttcta taaagttctt gagctttcat
     7801 ttgtgcactg gaagtttgtc gttcaatctt cataatatca tttaaatgag ttttgcattg
     7861 ttcataaaaa gaatctaaat catttgtatt tatttctgat aattctgttg caaatcctgt
     7921 ttctgaatta gcaaatgctt caggaagtct attcaacaat gggcgtattg gtaaaatact
     7981 tttagaataa atatctagtg ggaaatcttc actacctact ttaccatact tttcttctgt
     8041 ttttttccat atatttaaag catgctttaa tttttttggt gttacaactc cactactaat
     8101 ttcactatta ttagcataaa ttgttttttt atatttaata gcatctaaac cactcaattc
     8161 aactaccttg ccattattat ctaaataatt gataccctcg taagtaattg gaagaaaagc
     8221 catcaatgca gataaaacca atgccaacaa taataaaatt aatgtacgac gtgagtgcaa
     8281 cactcgttta atttccaaat aaaaaatacg catttatttc ccctccttac tataaactgc
     8341 ttctggaaat aaccataaat ataaatcttc tagtcgtggt tcaacagcaa tagagttatc
     8401 aatttgtgac ttttctgcca agtaacgaat agatacttga ttgttttttt cactacgctg
     8461 attgattata cgtaaattca tttcatattt tgaaatatct ctatttgata taagacattc
     8521 ccatactttg ccctctactt tcttaaccag tttatcggtt gttcccacat caagaatttt
     8581 tccacttttc ataatagcat tttgagttga tatgtactca atatcagata caatgtgagt
     8641 agatattagg acaacacgat tatgagaaaa ttctgaaata aaattacgaa atctcactcg
     8701 ctcaccaggg tcaagccctg ctgttggttc atccattata aggattttag ggtcatttaa
     8761 catggcttgt gcaattccca ctcgtcgttt cattccacca gataatttaa taatcttttt
     8821 attttttaca tcagataatg taagaatttc caatagatta ttaatttttc cttttacttc
     8881 tcttggtggt acatctttca atgctgctat atattctaaa taatctttta cagaaaaatc
     8941 ctgtgggaaa ccaaattctt gtggtaaaaa tcccagtaaa tttctgtact cagcacccat
     9001 tatataaata ttttttccat tatacagaat tttgccattg gtaggtttca taatgtctgt
     9061 aataattctc atcaaggtag ttttacctgc tccgtttgct cctagaaatc cccacacacc
     9121 tggtgttaag gtaaggcaaa catcttctac agcaatttta tccttaaatt tttttgtcac
     9181 atgttcaatt tttaattcca tatttgaaac tcctctcttt atctacttac ctgcaaaaac
     9241 aaaagtgtct tgcttagtat ttattttact aaacaaagac actcataatt ttaatattaa
     9301 atagataatt tcttaagaaa atcctaatat ttgatttata ttttactaaa tagatgtagg
     9361 aagcgatata gtaaatgttg tgacttcatc tttactttga acagtaatat caccaccatg
     9421 caaatttata atttccttag ctatagaaag ccctaaacct gcaccaccca tatcagattt
     9481 tctagcttca tctaaacgat tgaacttttc aaatatagca gaaagttgtt cttctggaat
     9541 tgtctgtcca taatttctaa aagcaatatc taccttgctg tctttatgaa ccgctgagat
     9601 aataatttct gtattaggat agctgtaagc aatagcattt ttcaagatat tattaaacac
     9661 tcttgcaagt ttttcagaat ctgcttttat tgttaaactt tcatttgctt ttagaatggc
     9721 tctatttcct ttttcagaca gcaatgggta aaattcatcc tttaattgaa gtaacatata
     9781 atataggtct actttttcga aattaatttt gatttgatta gaattatatc gtgttatttc
     9841 aaaaagttca ttgataagtt tttctaaata ttgtgctttg tcaagtgcaa cttcagtata
     9901 cttagatttt tgagaattat ccatattagg tatttctttt aataagctca aatacccaat
     9961 aactgaagta agtggtgtcc ttatatcatg tgctagatac atcacaaggt tattcttttt
    10021 attttcagcc tctttcactg cttgttgact taacaaaaca gacatcttaa tttggttcat
    10081 ttgttgctct atttccttta atggcttaga tagttcaacc aattcatcat tttgttgata
    10141 aatagtatgt gttgcattga tgatttctct taaataattc catggttttt tccaataaaa
    10201 attgaaaata gtgatatatc caattataaa aaccaaaaaa aataatacat ctcttctcaa
    10261 cttaagccat ttaacaagag gatttgaaaa atttgaaaac gtataatcag ccataaacat
    10321 taaaaccaat actatacctg tatagatagc taacattata tataatttat actttaaata
    10381 cgcctctgta tttttttctc tttttataaa tttatattta tctcttagtt tcctataagt
    10441 aaaattatta atctttttca taatactaca tcccttcttg acaagaaaaa taggtgtcaa
    10501 atcttgtaac caacacccca aattgttttt atatattttg cttcatctac agtatcacct
    10561 aatttttcac gaagatggcg aatatgaact gttatagtat tgttactttt attgtaatat
    10621 tcatctttcc agatagcatg aaaaagattt tctgaattta ccacagagcc tttatttttc
    10681 aataatacat gtagaataga aaactctgtt ggagtaagtg aaagagcttt accatttaaa
    10741 tggcattcat atgtttgggt attcaattcc agacctaaaa aagaaaaaat atgtgaaagt
    10801 tcttcaccag attgtaaact tccactgtat ttcttatatc ttctaatctg tgccttcact
    10861 ctagcaacaa gttccaaagg tcggaaaggt ttagtaacat aatcatctgc acctaaagtt
    10921 aagcctgtaa tcttgtctgt ttcttcatcc ttagctgtta acataatgat tggataagta
    10981 tatttttctc ggattttttg gcataaagaa aatccattta ctcctggcaa cataacatct
    11041 aaaattgcaa ggtcaaattc atttaaatta atgcagttta gtgcatcaag accagaataa
    11101 aatttataaa caatatagtt ttcatttgta aggtagactt ctattaaatc agctatatca
    11161 cgttcgtcat ctacaactaa aatttttcca ctcatagact ttaaacatcc tttctatata
    11221 tatttatata tattatacta aatttaa
//
LOCUS       DAMJVX010000117        10819 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.122, whole genome shotgun sequence.
ACCESSION   DAMJVX010000117 DAMJVX010000000
VERSION     DAMJVX010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10819
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.122"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(5..1063)
                     /locus_tag="PC407_002645"
     CDS             complement(5..1063)
                     /locus_tag="PC407_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDF2498078.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTIFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLEDGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINEIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            1130..1501
                     /locus_tag="PC407_002646"
     CDS             1130..1501
                     /locus_tag="PC407_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDF2498079.1"
                     /translation="MKKKDILLILGILVVIIACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            1538..2212
                     /locus_tag="PC407_002647"
     CDS             1538..2212
                     /locus_tag="PC407_002647"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDF2498080.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            2461..3300
                     /locus_tag="PC407_002648"
     CDS             2461..3300
                     /locus_tag="PC407_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2498081.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            3543..3639
                     /locus_tag="PC407_002649"
     tRNA            3543..3639
                     /locus_tag="PC407_002649"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3577..3579,aa:Sec,seq:tca)
     gene            3790..4497
                     /locus_tag="PC407_002650"
     CDS             3790..4497
                     /locus_tag="PC407_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDF2498082.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(4639..5475)
                     /locus_tag="PC407_002651"
     CDS             complement(4639..5475)
                     /locus_tag="PC407_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2498083.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHIIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNVIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            5663..6568
                     /locus_tag="PC407_002652"
     CDS             5663..6568
                     /locus_tag="PC407_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HDF2498084.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            6577..7761
                     /locus_tag="PC407_002653"
     CDS             6577..7761
                     /locus_tag="PC407_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2498085.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     RIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNTYINE
                     YI"
     gene            7876..9177
                     /locus_tag="PC407_002654"
     CDS             7876..9177
                     /locus_tag="PC407_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HDF2498086.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTLIESIFFLLEKISNLSFAYINIESPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            9131..10174
                     /gene="holA"
                     /locus_tag="PC407_002655"
     CDS             9131..10174
                     /gene="holA"
                     /locus_tag="PC407_002655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDF2498087.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(10361..10627)
                     /gene="rpsT"
                     /locus_tag="PC407_002656"
     CDS             complement(10361..10627)
                     /gene="rpsT"
                     /locus_tag="PC407_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDF2498088.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
BASE COUNT         4225 a         1119 c         1682 g         3793 t
ORIGIN      
        1 aaacttattg tttatttaat ttttcaatag aaaaagattt atctgtaagt ttaaaattat
       61 cttttatacc cttagttaaa tatactttct tatccttagt tatgaatata gcatctacat
      121 tatcaatttc attaattaaa tccatacctt tttcaagacc aagtccaaat gtagaagtgg
      181 ataaggcatc acaattaata gatttatcag aaattattgt aacactactt agattgtttt
      241 caaaaggata ccctgtaaat ggatttaaca tatgatgata tatttttccg tctttttcga
      301 taaatctttc ataaattcca gaagtaacaa cagatttatt tgtggtttct atgtttccta
      361 tggattcacc atttttacta gtggggtctt gaatgcctat tttaaaaggt ttatcatttt
      421 taccatcttc taaagtaaat atatttcctc ctaagtttac taaacctttt tttacgtcct
      481 ctgattttaa ataggctaca attttatcag cagcatatcc tttagctatg gcacctaaat
      541 caattttcat attttctttg gttaatttta tagatttatt tttttcatcc aatatgatgt
      601 tcttataatc aataagaggc agaagttttt gtatctcaga atcacttgga actttagctt
      661 tatctgttcc aatagcccat aaatttgata atggaccaat tgttatgtca aaatatccat
      721 ctgataattt ggaatattgt atagcttctt ttacgacgaa aaatgtatca tcagaaactc
      781 tgacaaattt tttaccagca ttatcattta ttttagaaac atcacttcct ggtatatgag
      841 tgctcatttt gttgtcaata tatcttaaaa ttgaatcaca ttcatttaag attttatcac
      901 ttttggattc ttttacatta aatatagtaa cttcgtttac tgtaccaagg tagtagctag
      961 ttttactata tttagtattc tggcttgaac tcctaaaaaa tgaaaataaa attaatgcta
     1021 aaatcaatat cacaaaaata aatgtgattt ttttcttatt catggtatat tcctcctatg
     1081 aaattgtgtt attattttat tataacataa tttcatagga ggacaaaaaa tgaagaaaaa
     1141 agacattctt cttattttag gcattttggt tgttattata gcatgttatg ggataataaa
     1201 tgttataaat tctaaaaatg ctggaaatat tgagatttat gtagataata aattgtataa
     1261 aactgtttca ataaatgcca aagaagaatt taaaatagag aatagagggg ggtataacat
     1321 agtaaagatt catgataaag gggttgagat agtagatgct tcatgccctg ataaggtttg
     1381 cgtacatact ggatttataa acaagccaag tcaaagcata gtttgtatac ctaatagagt
     1441 gagcataaaa ataaatacaa atgaaaagaa tgataatcaa gaagatataa tttctaaata
     1501 aaataataaa aataattgaa aggaattggt gataattatg gttaacttag gaaacgattg
     1561 ggatgaatta ttgaaagaag aatttgaaaa agattactac ttaaacttaa gaaaatttct
     1621 tatagaagaa tataaaacac gccaaatttt cccaaatatg cacaatatat atgaagcttt
     1681 aaaacacact tcatacaaag acacaaaagt cttaatatta ggacaagacc cttatcatgg
     1741 agataatcaa gctcatggtt tagctttttc agttcaacca caagtgaaaa ctccaccatc
     1801 tctattaaat atgtataaag aattaaaaga tgatttaggt tgttttatac ctaataatgg
     1861 atatttaatg ccttgggcag accaaggagt gttactttta aatactgctc ttactgttag
     1921 agcgcatgaa gctaattctc ataagaacaa aggatgggaa attttcactg atagagtaat
     1981 atctatttta agtgagcgtg aagacccagt tatttttgta ctatggggaa gtaatgcacg
     2041 taaaaaagta gagttgattg atacaagtaa acattatatt ttggaagcac ctcatccaag
     2101 cccattgtca gcaagtaaag gcttttttgg atgtaaacac ttctctaaga taaatgaaat
     2161 tttgaaaaag ctaggaaaag aacctattaa ttggcagata gaaaatatat aaaagtaaaa
     2221 ggtaacaaaa tgataacaaa acaacaataa acaatgacat tgttgtaact aatagaaata
     2281 tgcttaaata gttgaaaata ctatgtatgc atatttctat ttttttgtca agttgacaac
     2341 tcttgaaaaa atagttacaa tttagttaca caaaaaacac aaataaaaaa agaaacttac
     2401 aaagcagaat tttatgaagc aaaatatata gtgtaaaaaa ttttagggag gaaattttat
     2461 atgaatttta aaaaattgag taatgctaaa gtctcggtaa tggcagttgt tttatcagtt
     2521 ggaatattat cgggatactc tgcgcttaat aaacaagtaa cattagtagt aaggggagaa
     2581 gaaagaacta tatcaacatt tagttctaat gtaggagacc ttttagaatc tcaaaacatt
     2641 agctatgatg caaatgatat agtaacttta gatgaaaata caaagttatc taatggagat
     2701 aaaatagaag taattgatgt aaaggaaaaa acagttgtag aaactaaaga cattccattt
     2761 gaagttacag ttgttgaaga tggctcttta ttaaagggtg attctaaggt taaagaagaa
     2821 ggaaagtctg gtaaaagtga attaatatat aaagaaactt atcacaatgg aaagaaagtt
     2881 gaaaaggaat ttgtgaaaaa ggttgttaaa gctgagccag taaataaggt tgtaagcaaa
     2941 gggacaaagg tagaaatgca agtagcctca tcaaggggag aaagttcaag aagaatggct
     3001 tcatcatcat ctagtagcag tagtagtaac aatggtaaaa atatgagagt ggtagcaact
     3061 gcatatactg gtcatagtat aactgcaaca ggaacaaaac ctaagtgggg aacaatagct
     3121 gtagacccta aggttatacc atatggaact aaagtttata tacctcaatt tgacatggtg
     3181 tttactgcag aagactgtgg tggagcaata aaaggaaata aaattgatat ctacatgaat
     3241 gattcaaaaa cggtttataa ctggggaaga aaaactatag atatacgtat attaaaatag
     3301 ttaaataatt tatattttta ctggaaatta tattttgatt atatagagaa gaaactcttt
     3361 atagattctt aaaatataat ttccagtttt tattttggat atgtaataaa acaggtaaat
     3421 tttaaccatg tatagataaa agctatttaa attataattt actatcagat aaatctataa
     3481 aaattgttgc aaagagtcaa aatatatgat attatagatt tcgttgagaa ggatttcatt
     3541 atgggagtgg atgggtgccg gtgtgccctc tagtcttcaa aactagtatg aggggttaag
     3601 agcctcttag gtgggttcga ttcccacgca ttcccgccaa tatgcaaaag gtatctttta
     3661 attaaaagat acctttttaa atttatttta agataattta tttttaataa tatataatgt
     3721 tttgtattat ataatgtaaa ttttctaata ctattatata tttagataaa aatctaggag
     3781 tgtggaaaga tggaaaataa ttttacaacg agaactagtt ttctagttgg agatgatggg
     3841 atagaaaaac ttaataattc aaatataata gtatttggag ttggaggagt tggtagtttt
     3901 actgttgaag ctttagcaag agctggagta ggcaatatta ctattgtaga ttttgatgat
     3961 gtagatataa ccaatataaa tagacaaata ccagccctac attcaactgt aggaaggtac
     4021 aaagttgatg tcatggaaga aagaatatta gatataaatc ctaatatcaa tataaaaaaa
     4081 ataagaagtc tttataataa agatactagt gacgaaatac taactgaaag atatgattat
     4141 gttgtggatg cgatagacat ggtaagttcc aaaatacatc taattgaaac ttgtgaaaaa
     4201 aaagggttga aaataataag ttctatgggt atgggaaata aattagaccc aactaaaata
     4261 gtcgttacag atatacacaa aacaagtact tgtcctctag ctaaagttat gaggaaagaa
     4321 cttagggata gaggtatcaa aaagttaaaa gtagtatatt ctactgaaca accaatagaa
     4381 cttaagaaga aagtaatgaa tggtagaaaa gtaacacctg gtagtgtatc atttgtccca
     4441 tctgtaggag gtttaataat cgcatctgta atagtaaatg aactattagg acaataagtt
     4501 ttaaatattt taatttataa attttatata caaatttgaa ggctagaagc atgaatataa
     4561 agcaatttta tattcatact tttagcctaa ttattttata ttaccttatt ttatgtagtt
     4621 gtagttttat tttttagatt atgtatctat ttcataatta tattttgtaa atataatttc
     4681 tccatcacta taaaaaacaa tatctcctat tctatctgtt ctataaattg atacatggtt
     4741 ttgttttaga ttattaataa cctccctatg tggatgacca tattgatttt tatatccaca
     4801 tgatattgca gctatatctg gactaatttt ttttataaat tctaaagatg aagaagaatt
     4861 acttccatga tgacctattt ttataaaatt aatattattt aatttaaaag aatgtaaaat
     4921 ttctttttca ttttcttctt cagcatcacc cataaataaa aaagaattat cgttaaaagt
     4981 taatttgaaa actatagaat ttaaattaga ttcttcttgt atatatgatg gacttaatac
     5041 atatatattt atattgttat ctatgtttaa aacatcattt ttatagagat gctgaataga
     5101 taagttttta tccgtacaag aatttattag attttgataa gcctcactat cagttgattg
     5161 ttcaggcata tatatggagt ttacattaaa tttttttata acattatcaa gacttcctat
     5221 gtggtctgaa tctggatgag ttgctattat aatatcaata gtttttattc ttttttgtct
     5281 aagataacta ataattatat tttcgctatc ttcatcacct ccatcaatta atatattctt
     5341 atttgtgggt gtttgaacta aaatactatc accttgtcca acatcaatta tatgtataga
     5401 tagtagagat tttttgtcac agccgattaa aagtgatatt ataataagta ttgataaaaa
     5461 aattttattt ctcaataaaa aatcactcca ttcattacat gtttatataa atttatgatg
     5521 ggtaatataa gatatatatg acttattata ttttatattt taaacaatat aaatgaaatt
     5581 aataccataa taaatttgga gttttttata tatacaaaat aaaattttta aaattaagca
     5641 ataaaaaagg taggaggatt ttatgttttt caaagatatt gaactaaatt caaaaaaaga
     5701 gttagaccca tattttgatt tagtagatta tgaagcttgt gagtactgtt ttagtacttt
     5761 atatatgtgg caacatgttt ataagacagg atattatata ggtgaagatt ttgccgtttt
     5821 agttggggaa tacgaaggag atagtttctc aatattacca cttgctaaaa aagataaact
     5881 tccagaagtt gtagattttg tccttgaata tttttctaaa aataataaaa aaatatactt
     5941 aagaggtatt actactgaag tagtagagtt tttaaaagaa aaatatccag gtagatttga
     6001 gtatatagaa gaaagagatt tatttgacta tatttatgat gcagaaagtt taagaactct
     6061 agctggtaaa aagaatcaaa agaagagaaa ccatataaac tatttcttaa aagagtatgc
     6121 aggtagatat gaagctaaat tattagataa agaaaacttt gacgagtgtt tagtactaat
     6181 gaaggaatgg gaaagtaata aggaagaaaa taatgaattt gatgaaagta tggatgacga
     6241 attaataggt atcaaaaaga tatttaacca ttatgacata ttaaaagata aggtaaaagt
     6301 gtttggtgtt tatgtggatg gaaaattaga ggcttttagt ataggagaac ttttaaatcc
     6361 aaatatggca ttgattcata tagaaaaagc taatcccgat ataagaggtc tgtatccatt
     6421 tataaatcaa caatttttgg taagtgaatt taaggacgtt gagtttgtaa atagagaaga
     6481 agatttaggt attgaaggat taagaaaagc taaactttct tatcatcctt gcagatttgt
     6541 tgaaaagtac agtgttaggg aggcttaata gactatatgg agattaggta tgctaaagaa
     6601 gaagagatag aaagtataaa agagatttgg agttattgtt ttaacgatac ggagagtttt
     6661 atgaagtact attttaatga taaatataaa tctgaaaata ctgtagtagc tttagatgaa
     6721 ggaaaaatta tttcttctct tcagctaaat cagtataagt tattactaaa tagcaaggta
     6781 tataatacat catatgtagt aggagtttct acacttccag agggaagagg agctggatat
     6841 atgaacaaag taatgaaatt tactttaaac gaattgtata aaaaagggca attagtttct
     6901 atattgatgc ctattgacta tagactttat agaagatttg gatatgagca ttgttacgac
     6961 cagattgaat atactattaa cacagatgat ttaaaaaatt ttaagtcaag tggaaaaatg
     7021 ataaagtcta atttatcaca aattgatgat ttgatacgaa tagatagaac ttttttaaat
     7081 gaggttaatg gtaatgtgtt aaaagatgaa cattattatg aaaacttatt taaagagatt
     7141 caaagtgaag atggattttt atacattcat gaaggaaatg aaaaagatgg atatatagtt
     7201 tattttcttc aggaagataa aatgtttgtc agagagttat tttataaaaa tatagatgcc
     7261 cttaaatcaa tgcttaaatt tatatataat cacaatactc aatgtaagat agttactata
     7321 tctactccta caatagataa gataagattt atattagaca accctaaaga tagtgatata
     7381 aaaataaaac cttttatgat gggaagagtt ataaatgtaa agaagtttat tgaagatata
     7441 gatattgaga aggatattaa tagctcattt aatttattaa tagaagacaa gtttatagat
     7501 gaaaataatg gtctatttaa aatttctata cagaataaga aggtatctgt agagcagtta
     7561 gataaaaaag gtgcagaaaa gcctcaagaa gattttgata ttaaattaga tataaatacc
     7621 ttaacacagt tatcattttc ttatatagat gtaaatgaag ctattttctt aaatgatata
     7681 aaagatgttt cagaagaaac actagaaaca cttaattgta ttttttctaa aaaaaatacc
     7741 tatataaatg aatatatata ggtagaatac gttaaaagat tttaaaaatc ttttgacgta
     7801 tttttttgtt tagataacaa tttatcttgt ttaaaaattt tctatttgaa aataatctaa
     7861 ataatatact tttatatggg agtgattata attcaaataa gaagaccatt attaattata
     7921 ttaatgtttg tggtagctat atcatttatc attacaaata acacaaaaaa cgacgaagaa
     7981 cttaatgata agataataac tatacaaggg gttgtcaaag gaaaaattga aaagaaaaga
     8041 tacaatcagt acaaggtagg ggtttttcta ataaatgatt atacaaaaaa taaaaatttg
     8101 aagattggtc agaaagttaa tattacaggt aaattcaaaa gtttagataa aatgaagtat
     8161 gatgattttg attatggaag gtatataaaa agtactggat ataaaggtat agtgtatata
     8221 aataactata aaattattga taaaaataaa ctttactctt taatagggga aataaagttt
     8281 tatattagta aaacttatag gtacttatat aagaaaaact cagattttat aaattctata
     8341 ctattagcag aagttgaaaa tttgactgag gaacaaaaag aaatttttac tagaactgga
     8401 actagtcatg taatatctat atcaggtctt catacaggaa tattatgtgt aattatatcc
     8461 tttctattaa gaggtataaa taaattgtat aaattattaa tactgtcgat ttttataact
     8521 ttatattgta taatggtagg tgcgtctcca tccataatta ggtctatagc atttgttatg
     8581 attttttatt tatcaatatt tattgataga aagaaagatg gaatatcagc attgtctcta
     8641 attgggataa ttttaattat gaataacccc tatgttatat ataatgtaag ttttcaattg
     8701 tcttttttag ctacactttc tatattaata ttctataata aaataaatag tattataaaa
     8761 ttaagtatgg tatcattgac aatatcatca aatatattaa ctttacctat aatttattat
     8821 acatttaaag gtataccatt gctttcaatt ataggaaatt taattatagt accatttgtg
     8881 ggagtaataa tgtatttaag tattgcaagt ttaatcgtat ttaaagtaag tgtagtaata
     8941 gctaaaatta tatctttttt taatagtaca ttgatagaaa gcatattttt tttactagaa
     9001 aaaatcagca atttaagttt tgcatacatt aatatagaaa gtcctaaatt ttatattgtt
     9061 gttatttatt acataggagt gtttttttac atattttaca tagaaggaaa agagataaag
     9121 gagcaagaga atgaattaca aggatattat aaggaatgta aaagagaaaa actttgaaaa
     9181 aatgtatctt ttttatggaa gagagtatta tttaattgag aatgcaataa aagcatttaa
     9241 agatagtcta aacgaaggaa tgcttgattt taatttagat ataatagatg ggaaggaaat
     9301 agtcttaaat cagcttataa gctctataga aacacttcca tttatggatg atagaaagat
     9361 agtaataata aaagacttcg aactattaaa aggaaagaaa aagaatttta cagatagtga
     9421 tgagaaatac ttaatagagc atttagataa tattccagac actacaacta tagtttttgt
     9481 tgtatatgga gatgtagaca aaagaaaatc attagttaag aaaataggaa acaatggaat
     9541 tgtttttgat tgtgataagc tttctgatat ggatttattt aaatggataa aaaaatcttt
     9601 tgctttaaat gatgttataa tagataattc tcaaatcatg tattttattg aacaagaagg
     9661 atacagagat aagagcagtg aaaagacatt atctgattta gaaaatgaaa taaataaaat
     9721 aagttccttt gttggtaaag ggaataatgt aaccaatgac gtaattgata aattatctca
     9781 aaaaaaagtt gagaatgata tatttaaatt aatcgattat attggagaac aaaatgcttc
     9841 taatgcaatg aaaatattaa atgatatgat acaagaaggc gaatctgtat taggtatatt
     9901 ttctatgata gcaagacaat ttaaaataat tatgcaagta agacagttac aacttgatgg
     9961 atattcaaca aaacttatag ctgataaatt aaaaatgcat caatttgttg taggaaaagc
    10021 attaaagcaa actaaaaatt tttctgatga tataattgtg gaaatattaa actatatatt
    10081 agaaagtgat tataaaataa aaacaggtct tataagagat actttagcag ttgaaatgtt
    10141 ggttagtaga tactgtaaaa gagaagcaat ttaaaaaatc tatatattaa taaaaaataa
    10201 atagcaagat atgttatagt tcagagtaga tgtaagctaa attaagtttg atatacaatc
    10261 aactataata tgtctaacta tttatttttt attgtataaa tttacaaaat aaaaaacccc
    10321 tacatagggg ttattatata taagaaatat aaactggtaa ttaagcgttc ataccgttta
    10381 atttttgagc taatttagct acttttctag ctgcagcatt cttatgtata gttcctttag
    10441 aagctacttg gtatattctt ttttgagcgt attggaactt tgcaacagca tcctctctgt
    10501 ttccagctgc aacagcatct tcgaatcttc ttattgcagt ttttatttga gattttctaa
    10561 ctctatttaa agcagttttc ttttcgataa cacttattct tttcttagct gattttatat
    10621 ttgccaccgt gttcacctcc ctgtattttt ttatcatatt catcgtcaac atattagatt
    10681 ttaacagaaa taaatctaaa aatcaaggat tattatttat atattttaat aatgtttttt
    10741 attttgattt tttagactgt aaattgtgta taaataattt gcaatatctt gtttttaaat
    10801 tggtatattt tgtgtttta
//
LOCUS       DAMJVX010000118        10742 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMJVX010000118 DAMJVX010000000
VERSION     DAMJVX010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10742
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.123"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            123..1322
                     /locus_tag="PC407_002657"
     CDS             123..1322
                     /locus_tag="PC407_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HDF2498089.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKNIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEENKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            1374..1877
                     /locus_tag="PC407_002658"
     CDS             1374..1877
                     /locus_tag="PC407_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2498090.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            1984..2301
                     /locus_tag="PC407_002659"
     CDS             1984..2301
                     /locus_tag="PC407_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498091.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            2459..3418
                     /locus_tag="PC407_002660"
     CDS             2459..3418
                     /locus_tag="PC407_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2498092.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDIKRFKAII
                     ATSLVLGVLGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            3533..4318
                     /locus_tag="PC407_002661"
     CDS             3533..4318
                     /locus_tag="PC407_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HDF2498093.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDGLEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            4610..5929
                     /locus_tag="PC407_002662"
     CDS             4610..5929
                     /locus_tag="PC407_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2498094.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMSIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(6004..6432)
                     /locus_tag="PC407_002663"
     CDS             complement(6004..6432)
                     /locus_tag="PC407_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498095.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(6523..7899)
                     /gene="hydG"
                     /locus_tag="PC407_002664"
     CDS             complement(6523..7899)
                     /gene="hydG"
                     /locus_tag="PC407_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HDF2498096.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(7900..8985)
                     /gene="hydE"
                     /locus_tag="PC407_002665"
     CDS             complement(7900..8985)
                     /gene="hydE"
                     /locus_tag="PC407_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HDF2498097.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSPDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNKDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(9021..10229)
                     /gene="hydF"
                     /locus_tag="PC407_002666"
     CDS             complement(9021..10229)
                     /gene="hydF"
                     /locus_tag="PC407_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HDF2498098.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVNKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(10244..10495)
                     /locus_tag="PC407_002667"
     CDS             complement(10244..10495)
                     /locus_tag="PC407_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HDF2498099.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
BASE COUNT         3973 a         1297 c         1668 g         3804 t
ORIGIN      
        1 tagaagatgt tagtaaataa taggatgagt tctattttaa tacaattaaa ttaagcttag
       61 cttgaaattt gtatttaaaa agtaaagtag tctataatta agaaaatgga gatagaatta
      121 taatggaaaa gttatattat acaaatcagt atataagaga ttttacagca gaaataattg
      181 aaataaaaga agtagaaaat aagtatcatg ttttattaga taagacagct ttttttcctg
      241 gtggaggagg tcagtttgga gacctgggaa aaatagagaa tgatgatgta ataaatgtgt
      301 atgaagatgg aaaaaatata taccatgtat tagataaaaa acctataaag atacataagg
      361 taaagtgtag tattgattgg gatagaagag aagatggaat gcatcaacat tttggacaac
      421 atgtattatc tggatgtttt tttaaaactt ttaatgcaaa tacgacaggt tttcatttag
      481 gaaatgagta tagtacagta gatatagaag gtaatttaga agagaacaaa attagagaga
      541 tagagttgtt tgccaatgaa attattcgcc aaaatataca agtagagaca ttacttccaa
      601 cgaaaaaaga actaaaaaag atttggttaa gacgtgattt gccaaatact gatgaagaga
      661 taagagtaat aaaaataggt gatttagata cgaatgcatg ttgtggagtg catccaaaat
      721 caactggtga attgagaatg ataaaaataa agaaatggga aaaacataag tcaagtacaa
      781 gaatagagtt tctagcaggt aaaagggcag tggatgaagt attgaagagg gatatatatt
      841 tgactaaaat atgtagatat ttaagctgta gtgaggaaga tgctataaat ggtattgtaa
      901 atctaaatag taaagtggaa gaggctttaa ttaaaaaaag aaggcttgaa gaagtagtat
      961 ctacatatca ggtcaaagaa atgattgaaa aagcaaataa ggttggcaat atttcggtca
     1021 ttaaaaaaat ttatgatgat gaagatttga aatatgtaaa taagatagca aataaaattg
     1081 cagaatatga aaatagtatt gcattattag ctgttaaatt taatgataaa ataaatttag
     1141 tttttgcatg ttctaaaaat ttaaaagata ttaatatggg agttttatta aaggatgcaa
     1201 taagtcttat agatggcaag ggtggtggaa gtaaagtatt agctcaagga ggaggtaaga
     1261 ataatggaaa tttagaggct acacttgatt atgcatttat gaagatagaa aaaactctat
     1321 aaatatatta tataaaaaac cttagtagat aaaatagata tggaggaata agtatggagc
     1381 aacaaaagga ctacatagag ttatatgaaa atgaagaact aatagagata agagaagcac
     1441 gtatggatga tttggataca atagcaaaat ttaattataa tttggctaaa gaaactgaag
     1501 gaaaagaact tgatatggat gttttaacta aaggtgtaaa agcattgtta ttagatgaga
     1561 gaaaaggaaa atatcatgta tatacagtat ttgataaagt tgttgcacag attatgtata
     1621 catatgagtg gagtgattgg agaaatggaa atttcctttg gattcagagt gtatatgtag
     1681 ataaggaata tagaagaaaa ggtatcttta attatttatt taattatata aaaaatatat
     1741 gtgataaaga tgaaaatata gttggaatga gattatatgt agaaaaagaa aacataaatg
     1801 caaaagcaac atatgagtct ttaaatatgt atgagtgtga ttataatatg tatgaatacg
     1861 aagtaataca ttcgtaatat aaatttgtaa tactgttata ctgatgttat aaagtattga
     1921 ttatattgac gattatatga aataaactgg atattataat tagaattttt agggggtata
     1981 aaaatgggcg ttttaaaagg aatgttttat gtatttttat ttttagattt agtgtcaata
     2041 ttctgtttct ttttcaataa aggaaaaata gcatctaata aaattgtgtt taatgcaata
     2101 ggagtactta catttgtact ttgttttatg ctttttagtt attatcctaa taataatctt
     2161 attggaaaat ttattgcttc tctatttttt atatttggag ttgcaggagt tatgttgaaa
     2221 gaaaaaaatt ttttatatgc tagacttctt ttaactgtag ttatagtttt ttctacatta
     2281 agattgttcg ttattcaata aaatgtattt gtatgaaaat ttattaaagt ctacacattt
     2341 aagtgtaggc ttttttgatt ttttgtttga aaattaagga aattttaagg aaattttaaa
     2401 atcgtcttaa atatttattg atatacttaa aatattaata gcgaaaggag acttatcagt
     2461 gatagaaagt aaaaagtcag aaaaattgtt actgtcaatt atatacataa tatttttggt
     2521 atatatttta atgattataa gaataatttt atttagagat gtaccaattt acgctatttt
     2581 caagggaacg tttaggaatg taaatttaat tccattttat acaatatatc aatttatagt
     2641 agatagtaat cttgacttta tgaaagctac tgtaaatatt atagaaaata taggtatatt
     2701 tattcctatg ggaatttttt tacctatagt atgtaaaaat ctaaataaaa aaactataat
     2761 tatagtaatt atacttgtta gcctagcatt tgaattaaca caatatattt ttgctcttgg
     2821 aagtagtgat atagatgatg ttattttaaa ttcattaggt ggaataattg gaattaatat
     2881 ttacataaac atgaataaat tatttcctaa tgatattaaa agatttaagg ctataatagc
     2941 tactagttta gttttaggag ttttaggatt aggagttatt tctaaaaatt atcataactt
     3001 actaactttt aaatttagac ctgatagaaa aatatcaaaa atattaattg aagaaaatag
     3061 ggaaataatt aaagatataa ataaagatac agtagatata gttgggacat ttgagagctt
     3121 taaaaaagga ataataacta ttaaagcagg ttctaataac aaagtaaaga gaccagagaa
     3181 tttaattgat tctgatggaa atataagaat ttatttaaat gaaaatacaa aattagtatc
     3241 ttatataatt accaaagaat caaaaatgga tatagttaaa tatgaggaat ttgatgtgaa
     3301 gaatttagat ttattaagga gatatgatac tattaatgtt tggattgata aagaaaatca
     3361 gccaaaggat aaacatggta ttatggctag taaattatta attggattgt atgaataaaa
     3421 tattaaacaa gaataaaaga ataacaaata taaaaaacga aaaaactatt tctataatta
     3481 tttttacgtt gtgcataaaa taagaacatt agtgaaggag tagagataaa ttatggagat
     3541 aataaaatat atcttgtact tttttatata ttcattttta ggatggacag tagaaagtat
     3601 aggatgttct atagcaagca aaagaataat caacagaggt tttttaaatg gtccaatatg
     3661 ccctgtttat ggttttggag cagtaattgt gatatcacta ttaggaagat ttaataatgt
     3721 agtaatagta tttctattag gaatgatttt aactacgata ctagaatatt ttacaggatt
     3781 tattttagaa accttatttc atgctaaatg gtgggattat tctgacagaa aattcaatat
     3841 aaagggtaga gtatgtttaa aaaatgcaat atactttggt gtaatgtctg ttttaataat
     3901 aagatttata catccattta taaaatattt tgtaagtata attccatata gaatattaat
     3961 ttcaatggct ataattacaa cactttggac tatacttgat ttgattgtta ctattataac
     4021 acttaaaaaa ttagatataa agttaaactt actagacgat ataattacag accttaatga
     4081 tataaatgtg aaattggata aatttgatag aggagaaata caagctttat ttaaaacaat
     4141 aaataacgac ggattagaag ttagagaaaa aataaacaaa ataaatagta agttggaccg
     4201 tatagagtct aatattattt tacaaaaaag agttataaaa gcatttccac atataaaaca
     4261 taaaaaacaa caagagcaac tagaacattt taaaaaaatt attaatgaga aaaggtaatt
     4321 atacaattta atgaagacga atagaataat ggtatttata agattatttt cagaggactc
     4381 tataaaaata gaatagagtc ctttattttt tgaaaaataa tgtttatttg ataaaaataa
     4441 aatatattgt ttctaaataa caaatatttt gaaaattata aaaacatata ttgacaattt
     4501 gtgtcaaaaa tactaacatt aagtatataa agaattttat atgagaatat ctttataaat
     4561 cgcaataatt ttagaacata aatttattta aagaggggat gatttgccaa tgagcgagat
     4621 aactagtcaa atgataagta cagaagaaaa aaaatatgtt gctaaaactc aaaagatacc
     4681 ttattatcca gtagcattta aatcaggtga tggtgcaatg ctatatgatt atgaaggtaa
     4741 tgaatatgta gattttttag caagtgcagg ctctgcaaat gtaggtcatg gtaataagga
     4801 aatctcacaa gcagttaaag aacaaatgga tgatataaca caatatacac ttgcatattt
     4861 ccatagtgat cctcctgtaa aattagctga gaagcttgtg gaaatagccc ctggagataa
     4921 tgataaaaaa gtactataca gtgcaactgg ttctgcttgt atagatgctg ctataaaatt
     4981 agcaagaggg tatactggaa gaacaaagat aatatctatg tgtgaatctt atcatggaag
     5041 tacttatgga gctatatcta tatctgcttt aagtacaaat atgagaagaa aaatgggtcc
     5101 attgttacca gaagtttatc acttccatta tcctgataaa aatagaacag ctaaagaatg
     5161 tttagatgag atagaatatg cttttgcaca ctatctacca gcagaagagg tagctgcaat
     5221 atttatagag ccaattgcag gagatgcagg aataatagtg cctccagtag agtgggtaca
     5281 aggtttaagt aaaatatgta aagaaaatgg aatattactt gtaagtgatg aaatacaaca
     5341 aggtatggga agaacaggta agtggtttgg tatagaaaat tttggtgttg aagcagacct
     5401 tatagttctt ggaaaatctg ttggaggtgg attaccttta ggagcagttg ttggaagaac
     5461 agaaataatg caaagtcttg atgcaccagc acatttattt actttagctg gaaatacaac
     5521 agtgtgtgtc gctgctttaa aatctataga gataattgaa aaagaaaact tacttcaaaa
     5581 gagtatagaa atgggagatt atattaaagc aggatttgaa aaactaaaag aaaaatacga
     5641 tataattgga gaaataagag gaatagggtt atctatagga gttgatatag ttaaaggaaa
     5701 aggttctaat gaaaaacatc cagatgctac agctaagata tgttatagat gtatacaaac
     5761 tggattaata atgatattct taggacaatc tactttaaga gtacaaccac ctctagtaat
     5821 aactaaggag caagtagata aagcaatgag catcatagat tctgcaatag atgattattt
     5881 aaatggtaga attggtgatg aagtatatga agtaactcaa ggttggtaaa ataaatatta
     5941 aatgaaaaaa agagttatct caattttaga aagtaatttc tgaaatgaga taactctttt
     6001 aagttaccca caaataagaa aattgtttga ttcattaaag actatatttc ggatgtcgtt
     6061 tttgtgagta tcagagacag tattacgaga ataattacct ttattcgtac tatctgttaa
     6121 tttagttata attaagatag ctaaaagaat aagaattact gtgtataaag caataacata
     6181 tggttttctt ttatatttgt atttgttatt gaaaaaagaa tggccataat tatttttatt
     6241 tactttagat gtatttgtat tctttttctt gttacctttc tttttatcat ccttttttct
     6301 atttttaggg taaggaatga ctttgtgtgt attatcaagt ttgatatttt tatttttttg
     6361 aatattgttt tccaggctag cctttttttc tccaaattga atagttttat tggtattgtt
     6421 gttatctttc aatgttaatc tccctccctt aaactttccc tttagataat attactacta
     6481 acctatggaa tatacaataa ataatatata agtaataaaa ttttataggt ataaatctct
     6541 ttctccacgc tctaaggcag tcatattgtt agatactatg tttcttatat cttctctttc
     6601 tatcttacct atttcttctg cgataagttt tttacccata attttaagtt cttcatctcc
     6661 ataatcgatt aaaaattcat ttaatgtagt aagagcattt ggcgtacata cattatggat
     6721 ttgtcgactt ttagctaagc tcatgaatct atcgccagtt ctacctttac gataacaagc
     6781 tgtacaataa ctaggtatat atccatcagt aatcagttct ttaagcacct caacagggct
     6841 tctatggtct ccaacttcga attgttctgt attgtcacca tcttcatatg ctttatatcc
     6901 tccaacacct gttagagaac ctgcactaat ttgtgataca ccatatttta gtaactcatt
     6961 tctcatttca gcagtttctc tagtggacat tatgattcca gtaaaaggaa cagccaatct
     7021 agttatagct actatttttt tgaacatatc gtcagaaact aagtgaggaa aatcttctaa
     7081 gctcatacct tcagcttttt tcaatctagg gaaagatata gtatggaaac ctacaccaaa
     7141 tttttcctct aagtgttcgt tgtgcatcat aagtcctaga acttcaaatt tagggtcaga
     7201 taaaccaaat aaaactcctg caccaacatc atcaacacca gcttccatag ctctatcaaa
     7261 agctgttaaa tggtaataat agtcattttt tatagattga ccatgcattt ttataaatgt
     7321 tggtttgtga taagtttctt ggaataatat ataagttcct attccttttt cttttagtaa
     7381 tttatactca tctacacttg tagctgctat gttaacgttt acccttctta tatttccgtt
     7441 ttcattatat gttgaataaa ttgcatctaa gcaatctaag atgtaattta tatcacaatt
     7501 tttagggtct tcaccagctt ctagagcaag tcttttatga cccatttttt ctaatatttt
     7561 aacctcttcc tttatctctt ccatggtaag tttttttctt ttaaatttgt tgcatttact
     7621 aaaaccgcaa taaacacatt catttacaca gtagtttgat acataaagag gggcaaataa
     7681 aaccactcta ttaccatata tttcattttt tatttgccca gctatactaa ataatctatc
     7741 tagttgtttt ttatcttcta cttcaagaag tgtagctata tctttataac taagtttttc
     7801 ccttctatca gccttatcaa gtaccttttc aatatcatca gaagttgaat tttttgcatc
     7861 ttctaataaa gaattaataa gttcatggtt tatgaacatt tacaaacgcc tccattcaat
     7921 attatcaccg cgagaatagt caacttcaag tcctaatgac ttgatgtttt cttctaaact
     7981 ttggtgatat tcacaagctt cttgaccagt aaaggctttg ttatcgtaaa gagaatattg
     8041 ttttctaaat tcttgaggag aaaggtttgg cataattaca ttgcaaccag ctaaaagacc
     8101 tgcatttcta ccactagggt ttatagatgc caaggctgtt gtagcgggta ataaaacttt
     8161 tggaagtaga agtcttgtaa ttgaaagcat aagtaatgtc ttctctaaat ctccatgttt
     8221 ataatcctta aaaattgtat catggtgtgg aataaaaggt cctattccaa ccatatgagg
     8281 atttaactct tttaggtata ataaatcacg aacaaggtct ttgttgttct gaaatggtga
     8341 ttcaaccata aatccagcac ctacctgata accaatttct ttaaggtttt ttaggcattc
     8401 ttttctagtt ctaagctcaa tgtttggcgg atgtaatttt ttataatgac tgtctgtagc
     8461 agtttcatgt cttaaaagat atctatctgc accacataaa aagtattttt tatacgaatc
     8521 ataactcttt tctcctagtg acagagttat ggcacaatcc tcatatttat tttttatact
     8581 tgaaacaatt tcacatattt tatcatcagt aaaatatgca tcttcaccac cttgcaaaac
     8641 gaatgttctg tatcctattt tataacctat atcacaacat tcaagtattt catcaggtga
     8701 aagtctgtat cttatgatat ttttattaga agattgtatt ccacaatagt agcaattatt
     8761 tttacaatag tttgtaagct ctataagtcc tctcaagtag actctatttc cataatgttt
     8821 atttctcatg tcgctagctt gtttataaag atagtttctt aaattttgtt cgaagttata
     8881 ttcctcattg tcgatatttt ctattaaata tagtagttca tcaaaactta atgaattaat
     8941 gctgtaaagt ttatctataa agtatttaat cttattttta gtcatattca aatatgtaat
     9001 tattaagtat agttaaattt ttaataattg aaaagttcaa gtgatctttc caaaatacct
     9061 tttacatatg ctattaagat accatagttt acaataggta cattgaaaga ttttgccttt
     9121 tctattctag ataacattcc agctctattc atcatacaag ctccacagtg aactacaagg
     9181 gcatatttat ttatatcttc agtgaaggat actccagaac taaattcaaa agtaatattt
     9241 ttgccagttt tctttctaat catattagga atttttactg ttccaatatc atcagtttgt
     9301 ctatgatggg tacatccttc agccatcaag attttatcac catcttttaa attttctaaa
     9361 gcataagctc cattaatcaa ttctttaagg tctccctttt gtctagcaaa caatatagaa
     9421 aaagatgtaa gaggaatatc ttttggtgta tctttattta cttgtggaaa tacttgagag
     9481 tcagtaataa caagttttgg ttttttgcct aaattagata gagtttcttt taaactatct
     9541 tcttttgtaa ctatagaaat tgcaccatta tccaaaatat ctcttataac ttgctgttga
     9601 ggaagtataa gtctaccttt tggagctgct ttatctattg gaacaactaa aacaactaaa
     9661 tcatttggtt caattaaatc tgataccaat ttgaactcag tgctatcctt aggcaaaact
     9721 tttattattt cattttttaa attttcaatt ccaattttat ctgttgatga gacagaaaca
     9781 actggacatt taactttatt tttagttaag tttaaaattt ctgattgatt cttaatagta
     9841 tcaattttgt tgagaattag aatatgaggg atattcttat cattaaactt ttcaattagt
     9901 gacaaatctt cttgtgatat accaatttgg caatccacta ctagtaaagc tatatctgtc
     9961 ttttctaaaa catctagtga tttacctatt ctaagttcac caagttcacc aacatcatca
    10021 agaccagcag tatctataag aacacaaggt cctataggaa ggatttccat tggtctaaat
    10081 actgggtctg tagtagttcc agcaatatct gagactattg cagcactttg atttgttata
    10141 gcatttatta tgctagattt acctgcattt cttttaccaa agagacctat atgtactctt
    10201 accgattgtg gagtagaatt tagactcatt tgtactcacc accttaaact tctttttttg
    10261 atatactagt ttttacagat acatgaggta tgttgcccaa tttaccagtc aaactattaa
    10321 tagtatccat ttcgccaaca actgctatac atacgattga gacaccttct tcttcgcaag
    10381 gaatgcccat tcttccttta ataatacctt taaaacctga tacaacctca ttgaatttaa
    10441 attgacattc ttttggctct tccaaaatag cacttataac tgcaaccttt ttcataaata
    10501 tcctcctaac tcagaaaata tgtcttaaac aaatccaaaa tcaaaaaact tttctttgta
    10561 aagaaaagtt tctacaaaag aaaagttatt aattacacaa gtataatgtg ctatattaaa
    10621 ctatttcctt acagattaga taactctttt ctggtagggt tagcttgtaa aaatttaatt
    10681 taacaacaag taactgaaga aaatttttat acaatttata taaattataa taaaaattaa
    10741 ct
//
LOCUS       DAMJVX010000119        10704 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMJVX010000119 DAMJVX010000000
VERSION     DAMJVX010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10704
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.124"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            283..741
                     /locus_tag="PC407_002668"
     CDS             283..741
                     /locus_tag="PC407_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2498100.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFILGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLRTLKSL"
     gene            802..1077
                     /locus_tag="PC407_002669"
     CDS             802..1077
                     /locus_tag="PC407_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2498101.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1136..2410
                     /locus_tag="PC407_002670"
     CDS             1136..2410
                     /locus_tag="PC407_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2498102.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2540..3592
                     /locus_tag="PC407_002671"
     CDS             2540..3592
                     /locus_tag="PC407_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2498103.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     AVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDIDPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3592..4623
                     /locus_tag="PC407_002672"
     CDS             3592..4623
                     /locus_tag="PC407_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDF2498104.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKDIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4715..5527
                     /locus_tag="PC407_002673"
     CDS             4715..5527
                     /locus_tag="PC407_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2498105.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5530..6450
                     /locus_tag="PC407_002674"
     CDS             5530..6450
                     /locus_tag="PC407_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2498106.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6625..7071
                     /gene="rpiB"
                     /locus_tag="PC407_002675"
     CDS             6625..7071
                     /gene="rpiB"
                     /locus_tag="PC407_002675"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2498107.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7110..7775
                     /locus_tag="PC407_002676"
     CDS             7110..7775
                     /locus_tag="PC407_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2498108.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7885..9387
                     /locus_tag="PC407_002677"
     CDS             7885..9387
                     /locus_tag="PC407_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDF2498109.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTNIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNNSNLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9699..>10704)
                     /locus_tag="PC407_002678"
     CDS             complement(9699..>10704)
                     /locus_tag="PC407_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2498110.1"
                     /translation="AIVFLISIVVQAIIQYLVLIFFMYIYYSEAIAIVILFTLVNTVF
                     HFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEVIGDDEISTLAKSINNITGGLET
                     AVDANIKNERMKAELITNVSHDLKTPLTSILSYVDMLKNNDFDRETINDYINILDKKS
                     QRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQSIVEFEDKIQNSSLDFIIETPE
                     YAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYIDMFIKEENIILTFKNISNDKLNL
                     KPEELIERFRRGDISRKTDGSGLGLSIAQNIIELENAHMEIIIDADLFKVMLTFRRVN
                     "
BASE COUNT         4278 a         1222 c         1967 g         3237 t
ORIGIN      
        1 aaaaaccatt tgtaaaaaac aaaaaaatca tttctctaac aaaaaaaacc acaaaaaata
       61 taaaaaagta tggctagtta agtgattaac aagtaaaaat taaaataaaa atcaaaaaaa
      121 atttatcaat gaaagtcagc taaaatcctg gaagagagca ttattttcgg gaaaacattt
      181 tcttttgttt cacaaaaaaa gccacttttt ggcacgcaac atgctttata taaagtcaag
      241 aaaaactaag gtataagaca aaggcaatta ggaggtgtaa atatggagtt tataaatttt
      301 gtaagtgata aagttataga ccttaaaatg gattgtaaaa cgaaagaaga tttctttaaa
      361 gaaatacata ataaagtttt tgatttaggc tttgtaaaag aagaatttgg agaaaaaata
      421 ttagcaagag aaaatgtatt tccaacaggt ttaaatttag gtgattatgg agtagcaata
      481 ccacatacag atgcagaata tattaaagag caatttatat cagtctgtac ttttaatgaa
      541 ccagtagtat ttagttctat ggaagaccaa gatgaaaaag tacctgtaaa tttggctttc
      601 atattaggac ttaatcaacc acacagtcaa ttatctgtat taacagaatt aatgggaatt
      661 atgcaaaata aagagttagt tgataagttg atgagttcta ctgataaaga agaagtttta
      721 aggacactaa agtctttata aaaataaaaa agtactaaaa ataaaaaata taaaaataaa
      781 aattattagg aggaaataaa aatgaaaaaa atattagtag catgtggagc aggaatagca
      841 acatcaacag tagtttgtga caaggtagag agattagtta aggaaaataa tatacaagca
      901 gaagttatac aatgcaaaat agctgaatgt gcaacaaagc aagaaggagc agatttaata
      961 gtatcaacaa ctatactacc aacaacttat aatataccag caataaaagc aacagcatat
     1021 ataactggag taaatacagc agcattagat aaaaaaatat tagatgcttt aaaataatcc
     1081 tatcatagat acattgaaat aatattttat aaaacagaaa aaggaggatt tataaatgga
     1141 taaattatta gcagtagtac aatacatttt aaatatggga ccgacagtaa tactaccaat
     1201 aacaattgta gtactaggaa tgatatttag aattaagttt acaaaagctt taaagtcagg
     1261 tcttacaatt ggtataggat ttgtgggtat aaacttagtt gtaaacctat taacatcaac
     1321 actagggcca gcggctcaag gaatggttga aagattcgga cttaatttaa cagtaataga
     1381 tgttggttgg ccaacaacag catcagcaac atgggcttca ccagttgcac caatattaat
     1441 accagtttgt ttattagtaa acttactatt attatacttc aataaaacaa agatattaaa
     1501 cgttgatata tggaattatt ggcactttat agtagcaggt gcagttggat atatagtaac
     1561 aggaagttat gtttgggcta tattctgtgc aatactaatg gagttattag tactgtttat
     1621 tgcagaaaag acagcaaaaa atgttcagga gttttatgat ttagaaggta tagcattacc
     1681 aactggttca acagcttcat ttgctccact tggatattta gtaggtaaat ttgttgaaaa
     1741 aataccagga ataaataaaa tacaagcaga cccagactct atacaaaaaa gatttggtat
     1801 atttggagaa ccaatgatga tggggattat actaggtata atacttggag cacttgcggg
     1861 atatgatgta gcaggaatat tcaatgtagg tatatcaatg ggtgcagtaa tgttacttat
     1921 gcctagaatg gttagaatat taatggaagg tttaattcca atttcagaat caataagaga
     1981 tttcttacaa gcaaaatatg gagacagaga actttatata ggtctggatg cagcagtagc
     2041 aacgggacat ccagcagtta tatcaacagc tcttatactt gttcctataa cgttgttctt
     2101 agcagttata ctaccaggaa acaaagtttt accatttgga gacttagcga caataccatt
     2161 ttatatggca tttatagtgt gctttagaaa aggtaatata gttcaatcag ttataacagg
     2221 aacaatagta atagctatat cactttatat ggctacaaac tttgcagatg ttcatacaca
     2281 attattacaa caagcaaata tgatgccaga aggaacaaca atggttacaa gtatagatac
     2341 aggtggaaac ttgctaaaat ggggaatatt gaaattatct caattagttt cttcatttgg
     2401 aatattctaa tatgtagtta atagagatta ctctttttta gtaaagtatg cgaaaattta
     2461 acatttagca gctactaaaa aagagtaata ttttttataa atttttaaaa atacaaaata
     2521 caagaggagg agatttatcg tgaaagcagc agtattacat ggcacaaatg atatgagatt
     2581 tgaagatata gaaataaaac catgtgaaag tgatgaagta aaaataaaag ttatggcagc
     2641 aggaatatgt gggtcagatc ctccaagagt attaaaacat tggaaatatc cagttccagc
     2701 tataccagga catgagtttt ctggagtaat tgctgaagtt ggtaaggatg taaaaaatgt
     2761 aaaagttgga gatagagtag ttgcaattcc ttttatacct tgtaatgaat gtgaatattg
     2821 taaaagagga ttattttcat tgtgtgatga ccatggaatg ttaggagcta aaagttttgg
     2881 agcttttgca gagtatgtaa atataaaagc tacaaatgta cttccaatag gagatatgga
     2941 ctttgaagat gctgcaatga tagagccact agcagttgct atgcatggag tacttaatat
     3001 aggtgttcaa gttggagacg ctgtagccgt aatgggtagt ggaacaatgg ggcagttggt
     3061 aatacaagga cttaaaatag caggagcagg aacaattata gcagttgata tatctgataa
     3121 taaattaaga gaatcaaagg aattaggggc tgatataata atcaatgcta aagatattga
     3181 cccagtagaa aaaataaaag aacttactgg tggaaaaggc gttgacatag ctcttgaatg
     3241 tgcaggttca aaaataactc aagaacaatg tttacttata actaaaaaga aaagtaagat
     3301 aggattttta gggatagcat attcagatat aactttatca gaagaagcat ttgaaaatat
     3361 atttagaaaa gaactagaat taaaaggttt ttggaactct tattctgcac catttccagg
     3421 acaagaatgg actaagggaa tcaatctagt taatgaagga aagataaaat taaaagaaat
     3481 ggtatctcat agattctcac ttgaagatac ttacaaagca tttgaaatga taagagatag
     3541 aaaagaagaa tttaacaaaa tattaatttt accacaaggg gttgaaaaat aatgaaagca
     3601 gtagttaaaa ctaaaccagg ctatgataat gtagaagttt tagaagtaga agaaccaaaa
     3661 gctacaggag acaaggttaa aataaaagta gaatatagtg gtatatgtgg ttcagatata
     3721 cattcattta aaggtgaata tgcaaatata aaagccccag taacattagg tcatgaattt
     3781 tctggaattg ttgtagaagt aggagaagat gttaaagata taaaagtagg agatagagtt
     3841 acatcagaaa ctacttttgc aacttgtgag aaatgtatat attgtgaaac taaagattat
     3901 aacttatgcc caactagaaa aggtattggt actcaagtaa atggaagttt tgctgagtat
     3961 gtactatcaa gagaagaaag catacatgta cttcctgaaa atgtatcttt attatcagca
     4021 gcccttacag agccactagc ttgttgtgtt catgcagctc ttgaaaagac tactatagaa
     4081 agttctgata cagttttaat aataggacca gggccaattg gtttattatt agctcaagtt
     4141 gttaaatctc aaggtgctac agttataatg tctggtgtta caaaagataa ggaaagatta
     4201 gaaatagcta aaaatcttgg agtagataga actgtaaatg taatggaaga aagtttggca
     4261 gatgtagtga atgaaatgac tgatggaaat ggagtaaaca aaggatttga ttgttctggg
     4321 tttatgccag cagttaacga agccttaaga ctgcttagaa aaaaaggtac tttcgtacaa
     4381 gttggaatct ttgctaaaaa attaaatgaa atggaccaag aagcaataat ccaaagagag
     4441 ttacaatata taggaagtag atctcaaaag ccaagttctt ggattacagc attagattta
     4501 atggcaagtg ggaaagtaaa tacagaagct ttagttacta gaactgttgg tttagacgaa
     4561 tttaaagaag gtattacagc tttaatgaac ggggaagaaa taaaagtagc agtaaaatct
     4621 taaaaaaaca tttgtattat tttaaaaaaa attgtaaaat ctactgtagg ataaaaaaat
     4681 gtaaggtctt aaaaaataac aggaggaaga aagtatgagc actcttagag atcattctaa
     4741 taatattaga aaaaacataa taaaaatggt tgcagaagct aaaagcggac atccaggtgg
     4801 ttcattatca atagctgata tgttgacagt attatatttt gataagatga acgtatgtgc
     4861 agaaaatcca aaaatggctg atagagatag atttgttcta tcaaaaggtc atgcagcacc
     4921 agctttatat gctacattag cagaaaaagg atttttccca gaagaagaat taataacact
     4981 aagaaaattt ggttctaagt tacaaggtca cccagacatg aaaaaagtag caggaatcga
     5041 tatgtctact ggttctcttg gtcaagggct atctgcagca aatggtatgg cacttgcagg
     5101 aaaattagat aacaaagact atacagtata tactatatta ggtgatggag aaatccaaga
     5161 aggtcaaata tgggaagctg caatgacagc atcacattac aaattagata atttaattgc
     5221 atttgttgac ttaaatggat tacaaataga tggttctaat gaagaagtaa tgaatgtaaa
     5281 tccagtagat gacaaattca aatcttttgg atggaatgta atagtaataa atggacattc
     5341 ttttgaagaa ataggaaatg cagttgatga agctaaaaaa gttacaggaa aaccaactgt
     5401 aataatagct aaaacagtaa aaggtaaagg tgtttcattt atggaaaata atgctgcatg
     5461 gcatggaaca gctccaaatg cagaacaagc agagcaagca ttgaaagatt tagaaggagg
     5521 tctataggaa tgggaatagc aactagagaa gcttacggac aagtgttaaa agaacttgca
     5581 gaaaataaag atatagtagt attagatgca gatttaggaa aagctacaaa aagtatatca
     5641 tttaaagaag ttgccccaga tagatttttt gacatgggga tagcagaagg tgacatgatt
     5701 ggtacagcag caggtcttgc tacttgtgga aaaatacctt ttgcgagtac ttttgcaata
     5761 tttgcagcag gaagaggcta tgagcaaata agaaactctg tagcatatcc aaatttaaat
     5821 gtaaaaatag cagctactca tgcaggtgta acagtaggtg aagatggtgg tagccatcaa
     5881 gctatagagg atatatctct aatgagaggt ataccaaata tggttgtatt aaatccagca
     5941 gatgctttag aagcaagaca agctattctt gcatctattg attataatgg acctgtttat
     6001 ataagattag gtagagctgc aactcctgat gtaaacagtg aaaattataa atttgaaata
     6061 ggaaaaggaa ctgttttaag agaaggttct gacataacag taatagctac aggaataatg
     6121 gtagcaaaag ccttagaagc agcagaagaa cttgctaaag aaggtataaa tgtagaagtt
     6181 gttaatatat ctacaataaa accattagat gaagcattaa taaaagaaac tgctaagaaa
     6241 actggaaaag tagttacagc agaggagcac agtataatag gtggtttagg ttcagctgta
     6301 tgtgaagctt tagcagaaac taaagatgta gtagttagaa gaattggtgt taaagatgta
     6361 tttggtcaat caggtacacc agctgattta ttaaaacact atggtttaac tacagaagat
     6421 atagtaaaaa atataaaaga gttgttatag atataaatat atctaaataa caaattgatt
     6481 tgacataagt caattaaagc tgtcttaaaa atagaaagaa atttctacaa tgggacagct
     6541 ttatttttac aatactttat ttttatgata attataaaat agtctgaaag taatcaaaaa
     6601 aattataaaa agaggtgcta tttaatgaaa atatctattg gatgtgacca tggtggttat
     6661 gaattaaagg aatatataaa ggaaaattta ataaataaag gtatagaagt tgaagatgtg
     6721 ggtacaaatt caaaagaaag tgtagatttt ccaatatatg caaaaaaggt aagtcaaaat
     6781 gttcaaagta aaaaatctga tttaggcata ctttgttgtg gaacaggaat aggcatgtct
     6841 attgcagcaa ataagtttaa aggaatcaga gcagctgttg tttctgactg tttttcagct
     6901 aaggcaacta gagaacacaa taatacaaat gtactttgtt taggagaaag agttattggg
     6961 aaaggattag caatgagaat tgttgaagaa tggttaaatt caagttataa tggagaaaga
     7021 catgcaagaa gattacaaat gattgatgat atagaaaaag aaaatttata aatatatata
     7081 atatataatt tataacaagg gggagcgtta tgtctattat ttgtgcttca ataatgtgtg
     7141 cagaccaaat gaagttaaaa gaggaattag aagctcttga agaagcaggt gtaaaattac
     7201 ttcattgtga tgttatggat ggaatatttg ttaaaaactt ggctatggga ccagagttat
     7261 taaaatcaat aagtgaaaat acaacaatac ctttagacat acacttagcc acagaaactc
     7321 cagataaata tattgatatg atgtcttata taaagccaaa atatatttct tttcatgtag
     7381 aatcaagtac aaatgtaaaa gctgatatac aaaagttaag aaattatggt atagggccag
     7441 tgttagcaat aagccctcaa acaagtgtgg ataaaataga agaatatata agtttagttg
     7501 agggtatctt agtaatgaca gtaaatccag ggtttgcagg tcaaaagttt aatctttctg
     7561 tattggataa gttggataaa cttacagaga tactaaaaga ttatgataat cctccattta
     7621 tagaggttga tggaaatata aataaggata ctattaaact tatgaatggt aaaaaagtag
     7681 atatatatgt tgttggtact tcagctttat ttaatgataa gcctccaatt tcatataaag
     7741 ataaaataga agaactaaaa gaaagtataa agtaaccatg taataaaatt ataatatgtt
     7801 tctgtttaat agtaaaaatt taggtggaaa caagaaattt tataatattt aataaaaatt
     7861 ttggttgagt aaggagaatt ttttatggga gacctatata aactacaaaa tggtactgat
     7921 attagaggaa tagcgtatga aaatgattct aaagaagtta acctaactgt agaagaagta
     7981 aaaaaaatag ctaaagcgtt tcatatttgg ctaaaagaaa agactaagaa agataaagta
     8041 actgttgcca taggaactga ttctaggata acaggaagcc aatttagaag tactgttatt
     8101 gaaactttaa ctaatgatga ttgtaatgta attgattgtg agattgctac aactccagct
     8161 atgtttatga caactattat ggatggatac aattctgatg gggcgattat gattacagca
     8221 agtcacttac cttattacta taatggttta aagtttttca cagaaagtgg aggtttagaa
     8281 aaaactaata taaaagagat gttggatata gctgttaaaa atgatagtgt agactatgag
     8341 gaagtaaata aaaaaggtga agttatagta aaaaatttaa tagaagatta ttctaattta
     8401 ttgatagata aaattagaaa aggtgtaaat tcaagtaaaa attatgaaaa gcctttttct
     8461 gggcttaaga tattggttga tgcaggaaat ggagctggtg gattctttgc tgaaaaagtt
     8521 ttacacatac tgggagcaga tacgacaggc agtcagtttt tgaacccaga tggtatgttc
     8581 ccaaatcata tacctaatcc cgaaaacaaa gaagcaatgg aaagcatatg taaagctgtg
     8641 ttggataata atagcaacct aggaataatc tttgatacag atgtagacag agctgctatt
     8701 gtaggtaaaa atggcaaacc aataaataaa aatgctctta tagctgttat atcatcaata
     8761 gtattagaag aacatccaaa aacagctata gtaacagatt cgattacttc tgaaggactt
     8821 gctaaattta taaatgaatt acaaggaaga catcatagat ttaaaagagg atataaaaat
     8881 gtaataaatg aagctataag attgaatagt gaaggtgaag agtgccattt agctatagaa
     8941 acgtctggac atgctgcttt aaaagaaaat tatttcttag atgatggggc atatttaata
     9001 gctaaaattt taataaaagt agcaaagcta tccttggaag gaaagactat agaagaattg
     9061 atagaaaacc ttgaagaagc tcaagaagag aaagaagtta gaataggcat taacaaagaa
     9121 gattttagac catatgcaga ggatatatta aaagatttag attctcatgt taagagtatt
     9181 gatggatggt ctgtagcacc aaataatttt gaaggaataa gagttaattg tgatgaatca
     9241 aatggagatg gatggttttt acttagaata tcactacatg aaccattact agctttaaat
     9301 attgaatcaa ataaaattgg tggagcagaa caaatttatt ctaagttaaa acttttctta
     9361 gaaaaatatg atttaaaagg tctttagggt aaatagaaag aatatattta tattaaagaa
     9421 caaatgtaaa tttgtaccta agattacaaa tattacaaaa ttgtaatctt aggtaacaga
     9481 gatgtaactg attatggaaa cactatgtga tattatagat acagagagat gaaatatgtg
     9541 atgaattata gagaaatata atttaaaatg tattaatatc acaccaaaaa ggtataatca
     9601 aaaaatatgt aaatataaac cccaacattt agaaaaagac ctctaaggag gtttttttct
     9661 tgtttaaata acttaaaata tatttttata ttaatatatc aattaactct tctaaaagta
     9721 agcattacct taaataaatc agcatctata attatttcca tgtgtgcatt ttctaattca
     9781 attatatttt gagcaattga taaaccaaga ccagagccat ctgtttttct agatatatca
     9841 ccccttctaa atctttctat taactcttct ggctttagat ttagtttatc atttgaaata
     9901 tttttaaacg ttaatattat attttcttcc tttataaaca tatctatata gaccctagtg
     9961 ttaggcatag agtattttag tatattgcaa attagattct caaaaactct ccaagttttt
    10021 ttaccatcta tcatggtaaa aacagcatat tcaggtgttt ctattataaa atctaaactg
    10081 ctattttgaa ttttgtcttc aaactctaca atactttgaa ttaaaagttc tcttatatcg
    10141 gtttttgtta tattaaattc tacatctcca ctacttagct tagatgcttc aaatatgtca
    10201 tctattaata attttaatct ttgtgatttt ttatctaaaa tatttatata gtcatttata
    10261 gtctctctgt caaagtcatt attttttaac atatcaacat agcttaatat agatgtaagt
    10321 ggagttttta agtcatgaga tacgtttgtg ataagctctg ccttcatcct ttcatttttt
    10381 atattagcat ctacagcagt ttcaagacca cctgttatat tatttatact tttggctaaa
    10441 gtagatattt catcatctcc tataacttct aatttatact ttaaattgcc attttctata
    10501 ttttttatat tttttgatat gttatttatg taattaaact taacagataa aaagttgtat
    10561 atataaaaat gaaaaactgt gtttacaagt gtaaataaaa ttactatagc tattgcctca
    10621 gaataatata tatacataaa gaatatcaaa acaagatatt ggataatagc ttgtacaact
    10681 atagatatta aaaatactat agct
//
LOCUS       DAMJVX010000120        10692 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMJVX010000120 DAMJVX010000000
VERSION     DAMJVX010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10692
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.125"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(7..651)
                     /locus_tag="PC407_002679"
     CDS             complement(7..651)
                     /locus_tag="PC407_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2498111.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQRPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            836..1459
                     /gene="ytaF"
                     /locus_tag="PC407_002680"
     CDS             836..1459
                     /gene="ytaF"
                     /locus_tag="PC407_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HDF2498112.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLELNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            complement(1522..3432)
                     /locus_tag="PC407_002681"
     CDS             complement(1522..3432)
                     /locus_tag="PC407_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2498113.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILSYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(3969..5036)
                     /locus_tag="PC407_002682"
     CDS             complement(3969..5036)
                     /locus_tag="PC407_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDF2498114.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHTGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSEDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            complement(5282..6277)
                     /locus_tag="PC407_002683"
     CDS             complement(5282..6277)
                     /locus_tag="PC407_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HDF2498115.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRRNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            complement(6536..7795)
                     /locus_tag="PC407_002684"
     CDS             complement(6536..7795)
                     /locus_tag="PC407_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF2498116.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLTYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            complement(8034..8663)
                     /locus_tag="PC407_002685"
     CDS             complement(8034..8663)
                     /locus_tag="PC407_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDF2498117.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            complement(8856..9017)
                     /locus_tag="PC407_002686"
     CDS             complement(8856..9017)
                     /locus_tag="PC407_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498118.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            complement(9360..10520)
                     /locus_tag="PC407_002687"
     CDS             complement(9360..10520)
                     /locus_tag="PC407_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2498119.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALVVTQILHMLA"
BASE COUNT         3506 a         1752 c         1055 g         4379 t
ORIGIN      
        1 taaaacttac ttatcatttt taagtaattt tgtaactata tttaatgcta ctaaatcatc
       61 cataaatcct attgggaaca cgaagtctgg aatcacatct gttgatataa caaagtaaag
      121 tagcatactt ccaatcaata aatatttctt tattgtaagt ccatctgtac aaaaatcagc
      181 atacatttgt cttaatctat ctgtaaattg accttgtttt tcaatagaat ctattttttt
      241 attaaaatta tcaaaaagta attttttacc ttcatctgtt tggacatata acttacactt
      301 acttaactct ttttcaatat ttccattaaa gttcttatca ttgattaaat ttgcaaatcc
      361 aagaatatca tcaaagttac taaaatcact atctgcatta aatatttgat tattgctgtt
      421 tctaaattca tatcctgaag ctttcaacag ttcttctagg ttaatattta aatgtattgc
      481 catcttttct aaatgattta tatttggcct ttgtttatta ttagctatac gagatatagt
      541 agatttatca attccacttt ttttacttag ttctcccatt gataattctt tttcatctaa
      601 taaaactttc aacaaatcac cgaaattccc aaccttacta tgttgttcca ttattttcac
      661 ctgctttatt atgattggtt ttatattata tgatatgatt cttttgttat ttaggtgttg
      721 tatgatgttg aaattttatc aacaatttat tggattatct gtaacaattt atccattttt
      781 acaatactat aactaatata agtacaaaat ttaagagaaa ggatgttaat ctactgtgat
      841 tgaatcaatt ttattagtta cttctctttg tatagatgcc tttgtgacaa gctttggata
      901 tggagtaaat aaagttaaag ttcccttata ttcaaatata attatcagcg tagtttgttc
      961 agctatttta gcaatatcgc tttttgcagg tacaatagtt agagattttt tacctcaaaa
     1021 tataacaatg ttaatatgtt tctttatttt atttttatta ggaagtgcta gactaattga
     1081 aagttttttt aaatcttatt ttaagaaaaa atctatacac tcaaagaatt ataattttaa
     1141 tatgttagac cttgaattaa attttaatgt aagtatcgaa cctgatgtct tagatacaag
     1201 taaatctaaa gtgcttaaat ctgctgaagc atttacactt gcacttgctt taagtttaga
     1261 tggattagct atcggttttg gtagtggcct tgtaggtgtg aattatatac aagttattct
     1321 attttcttta atatcaaata taattgcagt attacttggt tgtttactag gaaatatgtt
     1381 tgttaaaaac gtaaatttaa acttatcttg gttaagtggt ttaatcttgt tagtcattgg
     1441 atttatgaag atatgttaat aagaaagcaa tctaaagata aacttttctc tttagattgc
     1501 tttcttttgt gctttttata tttattttct attctctcta atttctttct gaatcttttt
     1561 aagagctttt tttgaacctc tttgaatatc tttttctaat tttctttctt taaaactagt
     1621 aaataataca tctaaatcat acccttctgg atacaattct gatgctttta tgctaagttg
     1681 taatcttttt atatttactg aaataaaatt atcattaaac aatacttcta cattattaaa
     1741 cttgtccata ggcttatata caattccaaa atcatcttca tctagtaatt ttaccttatc
     1801 ccctacatta taatctgtat agcttaaaat gacttcagaa ctatttttat cttcaatatt
     1861 agattgtagc ttacttcttt taactaaatc taaattgtag tttctatcaa ttacatattc
     1921 tttagctcta tctaaaactc tattttttat tcccattttt ttagaaataa aaagagcatt
     1981 actatcttca gattttccaa tagttaactt atacaatggt tctaacgttt ctttatcaaa
     2041 catcatacca gcattttcaa attctggatg taatgtagca aactttttta tctctccata
     2101 atgagttgat gctatagtta tacatcccat tttgtaaaac tcttctaata atgatattgc
     2161 taaaccagct ccttcattag gttcagttcc acttcctatt tcatcaaaca gaacaagagt
     2221 agaattattt gataaactca taatctctgc aatatttttt atatgtgatg aaaaagtact
     2281 taaggcattt tcaatacttt gattatcccc tatatctaca aaaacttttt caaatacact
     2341 aaattcacta ccttcttttg cagaaatatc aaatccacat tgaaccatta aagttagtaa
     2401 accaacagtc ttaagggtta cagtttttcc tcctgcatta ggacctgtta ttataagact
     2461 tctataattt ttgcctattt caaaatctaa aggtactgca tctcctgtta gaagtggatg
     2521 tttaccttta attattttta tatagccatt attatttatt tttggagtaa ttccttttat
     2581 tttttgacta tacttagctt ttgcaaatac catatcgtat tctgaaagta tctctatatt
     2641 taattttatc tgacttatct tctcaaatat aagttctgtt aaataagata atattttgta
     2701 ttcttcaata gtttcatcag cttttagcat agttaactct gtacttaaat ttgaaacact
     2761 aataggttca ataaatacag tatttccttt tgatgatgta tctaatatag taccattaac
     2821 ttcattttta taagaagatt taattggtat tacatatcta tcatttcttt tacttattat
     2881 aaactcttgt atatactttt tatttacagt tgatgttata aatttattta atctatcttt
     2941 tatctttcct tctgtaattt ctatatttcg tcttatcttt tttaattctt tactagcatt
     3001 agaatcaact ttattagatt ttatacagta atttatctca tcttctatac ttttacattc
     3061 tgttatattt aaagcataag aacttaatgt aggtgaataa aattcttttt ctaacataaa
     3121 agctttcatc ttcctacaac ctcttaaaaa atcttcaata tttacaagtt caacaggttc
     3181 tattatcatt cctttttcaa ttttatctat tgtagaacct gcattaaaca atccttcaag
     3241 tggtatgtga ttactatttt caattatctt tctagcttct ttattttctg ctaatttcct
     3301 tcttactact ccaatattcc cactaggagt taatttatct attaaatttt ttcctagcga
     3361 acttacacaa tgtattttta ttaagtcttt aacttcattt agttgcaatc tttcaaatgt
     3421 attactattc attatttttt ctcctttatt tataagtcat cgtcttttaa atcaccctat
     3481 catttacttt gataatagtt attttgcgaa caacttaata aataaggagt tattatatat
     3541 caaactcctt atttattaag aagtttaact ccggactcat taatgttagt attttttttc
     3601 ataaaacact tctccttttc tattcttatt taaacactta taccagtgct tctaatatta
     3661 ttccataaat tatatattta tgtcaataaa aataatgtta ttaatgtttt tgtacagtga
     3721 aaatttttat agtgaaaata agcttatact ttcatttata ttttggaatt acttatattg
     3781 tcatcaattc atgtatgtgt agtttccaat acaatctaac tttgtaatca ttatatatca
     3841 aagaatttag ctctataaat tatcgtctct ataaaataaa aaaatagcta ataataataa
     3901 aacactttta aatgttccat aaaattagct atttataata taattttaat cttttttaac
     3961 tttaaaaatt atcctttata agtaatcaat ggcttcatag ttgccactac ttgaataagt
     4021 tcatattcta tcaaactttc aataatctgc tctatattct tatatgcctc tggagcttct
     4081 tgaaataata aatttacatc attacatata atctgacttt taaatttagt ttgttttata
     4141 gtatctttac tatacttact tttaaggcgc gatttacaaa ctgaacgaga ccattttcta
     4201 cctgctccat gtgatagaga atataaagat atctctgtat tttcagtagg cataactata
     4261 tatgatagac tgcctcttga acctggtata atcacagctc ctctttcact tgaaacagca
     4321 ccttttctat gtataaattt atcttccctt ttctctataa agttatgatt tatagagaat
     4381 attgtttcaa catcttcaga tgtacctatt gactgcataa tttttttact gatagcttca
     4441 cgattacgct ctgcccacat taaagcatta tcatgttttt taatataatc tattgctttc
     4501 tcactttcaa cttcaagtcc atctttatca taaaattttt ttaaaatatc ttctccataa
     4561 tttcttgacc cacagtgaac taacatcata atatcatcag atgaaaattt taattgttca
     4621 aattgttctt tattataaat ttcacttata cattgaattt ctacaaaatg atttccactc
     4681 cctatagttc ctaaatttaa aattggacat tcttctttat atgtgttttt tatttctata
     4741 tcggacaaat ctttaatctt actaagtgac ttaatccatt tgtctttttt aaatttcttt
     4801 tttagaaccc cagttttaaa taaagtcatt ccgcatccta tatcatttcc aattatatgt
     4861 ggataaataa tatttttagt ctcaactgct aaacctacag gtattttacc tgtatgtaag
     4921 tcaggtaatc ctactactct taaaattcca tttaactttg aaatatcttc tagttgtttc
     4981 cttgctatat cttcaatcca acatttatta cttgctatta tttttatatt gctcataatt
     5041 tgctcctttt taatattctc ctgcctttaa aaagtagcaa aaagctgtga cctttagcca
     5101 cagctttttt attctaaact gtaaaagcag gtggctattt gttaaaatac tcctagacgt
     5161 ttaatacctc attatttttt gttaacataa taaccacttc ctttcataat ttaagactac
     5221 actaaaattt ctatgtatat catttataga cattgatttt aaaatactta atatacaatt
     5281 tttatattat ggaactaata ataatcattg aaatcatgac tatagctaga ccggcaacaa
     5341 caagtttttt atgtaatttt tttctcattt tacttttata ttctttttta acttgtataa
     5401 ctttgtcttg tttctgttct tcttctttct catcttcaac ttctatatat gctttttcat
     5461 acattacttt acttaaatca ataataatat cattggctct agtatactta ttatttcttc
     5521 caagaagcct ttttataaca tttataagtt ctgtatttcc attaattgct tttatactat
     5581 tccaatctaa tcccttatca atagattgta gcattttttc ttctgaattt gattctccaa
     5641 aaggcaattt tttaaatata gattcaaata gaataagacc tgttgcaaaa aaatcacttt
     5701 ctttatcagt ataatttata catagttgat gtgggcataa atatctcctg tttccatagc
     5761 ttcttgtatt aaccccatta tttgcttttg taattccaaa atcacaaatt tttatattgt
     5821 attctgtatc tactattata ctactcgatt ttaaactacc atgatatgaa tgattcctat
     5881 gtatcatttc taaagctttt aaaacttgta tcattatgtt tacaattgct tctaaatgaa
     5941 gataattacc tagaattaat tcatctagtc cgataccatt aaagtcttca cttacaacat
     6001 aatataatgt tgtttcttct gtacagtgaa tcccaacatc tattattttt agtatataag
     6061 gagagtttat ctcatctaaa gcagtacttt catcaattaa atttgaaaca aaatcttcac
     6121 aaatataatt attgtgcttt ataactttta ttaacacatt tctcctgtaa aaaacatctc
     6181 tagctttata gattttgtta atttctaaaa cgtcatcaga atttactacc tcatatctat
     6241 tgcctataaa attcaaccat gtcacctcct catacatttt atataactaa atctatttac
     6301 aattatacca atatatagga atattttcta tatattaatg attgtaatat taaaacaaga
     6361 actaatagtg ttaattttta caaaaaaggc tgtctcaaaa tagagatacg cttcttttgt
     6421 attgttgaaa tgatgaaaat atatttgatt tcacataaaa agagtgtctc ataaactaaa
     6481 aagttcattt tgagacactc ccttttatat tacaaatatt tctctaaaaa attatctatt
     6541 tttttaattc tttataagaa ctatttgata tatgttcttt ttttatgacc tttccacttg
     6601 aatctttaaa tattcgatat gtagaatatt tattctgacc agtttgtttt acctctatag
     6661 atatatttgg tttgtcacca gaattaccat agattactga tgatacactt cctccacctg
     6721 cataattttt aatatataca ggatgcttat atggattttt aaactttaag tttaatcctc
     6781 catcgctcac agtagcatct cttcctttag gcacataagt agatgcagat gaatgatttg
     6841 taacttgagt tatctccata cccgaaagaa gagccgcatt ataaactgtt gaagatgttt
     6901 gacaaacacc tcctcctgct gattgttcta gcttaccata aactataaca ggtgcatctt
     6961 tgtaaccatt tgctttatta ctaggtcctg taagtttatt gtatgaaaat tcttcacctg
     7021 gcattaataa tacatcacta gtttttcttg ctgataatcc tacattatat gacctaccct
     7081 ctacagcttg tgaataagta gttgaatatt gacctataac agaatttaca gtttttactt
     7141 gttcagttgt tatatctggt ttttgaagat ttactttaag ttctaaagtt ttatgctctt
     7201 tattttttat catattatat atagactctt tatttgctgc caaatcaaat tctctaccta
     7261 caacagagtc tgtataactt agtccaccac ttccaacact aatagaagca tttttcatct
     7321 taacatttat actatctgct attgaagaca ctttggaact aagttttgcc tcatcatatt
     7381 cagcttttat cttcatattt gccttattca ctcttatatc aatatatttt tttaagtttt
     7441 gaaaatagga cccttttttt gtactttcat aagcatcttt tacaacatct tctgtatcat
     7501 attttaaatc aatatctttt ggtgatattg tatatttttt cccatcataa gtaagattta
     7561 agtcttctgg agtatactta tctgttatag cttttaatgc ctctgcttta gtcatatcag
     7621 atacatttac tccttctata tatacatttt tagcgatttt cccattgtac agaaatttac
     7681 tgttaaaaaa ataagtaaat ccacccatta ttagaactaa agctgccaat acaattaaaa
     7741 cacttcgttt tttttcttta cctaatcttg aaactcttgt tgttcttctg cccataactt
     7801 cacctcattt tgacactagc ttaaatttgc ataatagagt tcccatttca aaactacctt
     7861 agattatatt tgaatagctt ttgttaaaca aggaatttaa tcaaatgtaa ctttattgta
     7921 aatttatgtc ttataattag ttttattttt gatatttttt attatatttc ttttactatt
     7981 tcttaacaaa aaattgttta ttttataaca actaatttca tttatattga aatttaaatg
     8041 taacctatta caggataatg gtctgacagg taaatttttt ctactgtgta tccttttaat
     8101 tcagtatttt tattaacaaa aatgtaatct attctagagt ttgatttttc aaatgttggt
     8161 aagtaagact tgtttagtga aatagccata tcattaaaca tacttaaaaa gacatttttc
     8221 tcattaaaat caccacataa aataactatc cccactaatc tgttcctata gtctattatt
     8281 tcgtctaatt gttttgctct ctcctgccta tcaagtccca agtgagtatt tattacattt
     8341 ataattcttc catatgcatc tatagttata caaagagctc cccgttgttc ttttttgcta
     8401 gttaagaaaa aatgattact gtttaacatt ttagatgttg taaatgtaca aataccatat
     8461 atcatattta ctttttttac atttgtagca aagacaccat ccatatttaa aacagctttt
     8521 aaagctaaaa attgaggata taatacttct tgcaaacaaa ttacatcaca atccaattgt
     8581 tttaaatata accccatttt agttagagtt aatctattat ttgaatccat acctttatga
     8641 atattgtagg ttactatttt cataataata tcacctctta tgcaatataa tacttactaa
     8701 aatttaaact tatttacttt tcacttaaga ccattattta tcttataaaa ttgagtactt
     8761 ataaacttta tcacaaaaaa acctaactta agtatttata ttctacataa gttaggtttt
     8821 ataaactatt ataatattta ttatttcatt catttctaat gatgattctc cttttttatc
     8881 tttttcaatt tgccttcact aacaacaaat aaaaatccca tcttcttgta tatttcatcc
     8941 aattcaatta aagttacttc accatttttt aaatttgtta atgttatatc ttgtattctt
     9001 cttgatggaa ttatcataag ttatcaacac tcctttaaat tctttttaat ctttatattt
     9061 aaatatattt atgcattctg atatatattc ataaattatt aaaataaatt caaaaaacac
     9121 ccgttctgca aacattcgtt ctgtatattt attatacatc atacagacgt tcgattcaat
     9181 ataaaaatta aatatatctc gtttttagta aattttacaa tattatacac taaatatgtc
     9241 aaaacatctt cttgaaatag attatcttgt caaacaaaaa aaaccatttc agtaaaagct
     9301 tctgcttaaa ttgaaatgac ttttcttatt taaattatta attttatacc atatttttat
     9361 taagctaaca tatgtaatat ttgagttacc acaagagcac aataagtccc tataatattt
     9421 cctagaagcc ccataagtac acctactggt attaatacag gattgtgaac cccagcaatc
     9481 attggagctg tagttactcc tcctatatta gctatacttc ctacttcaca agtaaataaa
     9541 tcaagtttaa atagtttagc aagccctata aatgaaatag catgtacaag gactactacc
     9601 aatccaaaca ttatgtaaac aggtgcttct gacaatccaa agaaatttgc tttactacct
     9661 gtagctgcaa ggagtaaata cataaacaca tttcctacca gtgtactccc tttaagcttt
     9721 gatatttttg tcatacccaa taatattgat actgtagatg caacaagtac tacccacata
     9781 gtttcattaa gtatacctga agttggaagt atcttggata caatttgtgc aaaagacgca
     9841 actccaaacc caacagaaag taaaatcata agttctacaa acgtaggtgc cgtattatct
     9901 ttattttctt tttcaagttt tgatgtaacc tcatctataa aacttgtatc gcactttgtc
     9961 catttgttaa atttaggtcc aaatttcaca gacattagac aaacagacat ccatactgta
    10021 tatcctatag aacccataag tattgcataa ctaagtcctt cttctgcaac tcctagagca
    10081 ttagctgctg caatcatatt tgatgagcca cctatccatg aaccagatgc tgccccaaga
    10141 attttccatg aatctgcacc cattgaattt ttaaaaatta tgaatgctat aacaaatcca
    10201 agcataaatg taaatgtagc acagaaaaat gataataaca ttttaggccc catctttaat
    10261 atttttctta aatcacattg aattaacaag aacactaaca ttaatggaag gaagttcact
    10321 gttattatgt tttgatacgt tccaacatct tcattctgtg caaataaacc aaatgatcca
    10381 agtatagctg aacctaagta tattaaaagc attggattaa taacatcaaa taccttccat
    10441 ttcaatttat caaccataaa aaaaattaca cctacgaata caaccaagaa actacaatat
    10501 acaaatccag tctcaatcac attatacaca ccctttcttt tcctaattaa tttgattttt
    10561 atgatttcta agtaaagact ttttcagata atagacaatt aaaataaata cttttatcta
    10621 tatcatgcta attaaataca tatataccta actattgaca tatattaata aagatttgca
    10681 cattaattac tt
//
LOCUS       DAMJVX010000121        10531 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMJVX010000121 DAMJVX010000000
VERSION     DAMJVX010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.126"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(116..685)
                     /locus_tag="PC407_002688"
     CDS             complement(116..685)
                     /locus_tag="PC407_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2498120.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            888..1115
                     /locus_tag="PC407_002689"
     CDS             888..1115
                     /locus_tag="PC407_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2498121.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(1334..2077)
                     /locus_tag="PC407_002690"
     CDS             complement(1334..2077)
                     /locus_tag="PC407_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2498122.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYITDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            2245..3117
                     /locus_tag="PC407_002691"
     CDS             2245..3117
                     /locus_tag="PC407_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2498123.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            3117..3782
                     /locus_tag="PC407_002692"
     CDS             3117..3782
                     /locus_tag="PC407_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2498124.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            3803..4660
                     /locus_tag="PC407_002693"
     CDS             3803..4660
                     /locus_tag="PC407_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2498125.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGSYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(4816..7362)
                     /locus_tag="PC407_002694"
     CDS             complement(4816..7362)
                     /locus_tag="PC407_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2498126.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(7362..8048)
                     /locus_tag="PC407_002695"
     CDS             complement(7362..8048)
                     /locus_tag="PC407_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498127.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(8131..9120)
                     /locus_tag="PC407_002696"
     CDS             complement(8131..9120)
                     /locus_tag="PC407_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2498128.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIVCEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(9107..9793)
                     /locus_tag="PC407_002697"
     CDS             complement(9107..9793)
                     /locus_tag="PC407_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2498129.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(10293..>10531)
                     /locus_tag="PC407_002698"
     CDS             complement(10293..>10531)
                     /locus_tag="PC407_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498130.1"
                     /translation="IEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRTAIEF
                     STEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT         3715 a         1744 c         1540 g         3532 t
ORIGIN      
        1 aatactctat aaaagctaaa aagttttcta ttcgctacat ctaaaaataa ttagatagag
       61 gtataaatgt atttagttta tttatacctc tagtctttta taaattatat ctctactaaa
      121 ttgatttttt agcacgaata agtgtgctct tggagatacc agtcatcttc acaacctctc
      181 tatatggctt agaatttaat aaccccaatg catattgtat ctgttgattg ctgtatttct
      241 ttggcctacc atctttaaag tcctttttct ttttagcaaa ttcttttcct aattgagtac
      301 gttctattat catatttctc tcaagctctg caactgcaag taatgtagtc aaaaagaatt
      361 tacccattga agtattttct aaaagaccta tatttaaaat atgaacactt acttctttag
      421 caaataaacc ttctattagt tgtatccctt ctgatgtact tctagctagt ctatccagct
      481 tagttgcaac aagtaagtct ccctttttta gagatgatat aattttttca aatttaggcc
      541 tgtttgtagt agtgcctgta taagtttcct ttattatttt agcatcatta tatttattca
      601 aaatttcttg ctgttgctga tacaaactgt tgttgtctgt ttgatttttt gtacttacac
      661 ggcaatatcc atatataata atcatcactt cctaacataa agattttgta cagaagttac
      721 ccagaagctt gtttttactt atctttatcc attttactaa aacgattttt gggtgtcaat
      781 actgttaact tttgacacca taataattcc tttataattt gtttgactat gtcaaaaatt
      841 accaatataa tgaaattaat gagtttatac aaattggagg aaataatatg aatagtctgt
      901 taaaagaagc aatagatgaa ttaaaaaaca tccattgcgg ggaagttttt ttattaaaag
      961 aattgttcaa aggctatgaa tggaacaggt tggagatgaa tattagattg aaattaggaa
     1021 aacttttttt gtatgaagtg gtaactaatc ccaatttaaa tgtagaggtc atcaataaaa
     1081 attctgctta tcagcaaatg tataaaaaaa aatagtgact taaatgtata taatagtata
     1141 atcttcttat tgattcaaaa gttaaaactt ttaatacaaa acaatcattc tattttaaca
     1201 tttatcaata tgtctaaatt tatataatta acacatacga atagtattgt ttaaatcaca
     1261 ttatttaaga tagataaatc ttaaccctac tccagcataa atactaaaat agggcttaga
     1321 attatctata tttttatccc ataaaatgct tagctcttcc tcttgattga acatatatat
     1381 ctggggaaat acaatagaag cgtaaattta taaaatttcc aaagtcagaa aacttcagtt
     1441 caaacttagg tataccaaaa ctttcaggtg ctatttcatc aacgccaaaa tagatgataa
     1501 ttccattatc tgtaatataa aatgcttggt cttcaggtat aactaactca acattagaat
     1561 aatacatttc tctattttca tttatttgag atttaatgta atcagtaatt atattaatat
     1621 aatcaactcc atctctaaaa atgtcattaa gtaaaagttc ttttccagtt gtcaaatcta
     1681 tattgtaatt attcaatgta ttgtatttag gcccatagtt attagcaaac cccataaggt
     1741 ttaatatcat actcagtaca taatttttat taaaagtaac tgcgtaactg gtagataccc
     1801 aatagctttg ttttggaagt gaattttgag aagctgcgtt attatattct cgttcttctt
     1861 cctgtaagcc atttttaaaa ttatttacag tcattcttac agcattgttt atgttattta
     1921 aaataactgg gtttacatac gtggattgat ttctaatatt gtatataaca ggatacttta
     1981 catcatatga aaaatactta cttctttcaa aatcttcatt aaattgtaca tataatatat
     2041 tatatgtaca ggtatttgta gattgataat tttccattaa aatcctcccc cttttataaa
     2101 aaaatcagat acaatataat atgcttaaaa cagtaataat gttaatagta aatgtaaaga
     2161 atagaaagta tctaaaacag caaaaaatga ctaattttgt ggtataatac ataggattga
     2221 gagttatttt ataggaggaa aaatatgtta ttattagcag ataaatggaa agattatgaa
     2281 cttatagata tgggaaatgg agaaaaatta gaacgttggg gaagttatgt acttagaaga
     2341 ccagacccac aggttgtatg gccaatggaa tctgaatggg ctttatggaa aaaccctcat
     2401 ggacattatc acagaagcaa taaaggtgga ggtcaatggg aacacaaaaa aagatatcca
     2461 gaacaatgga ctataaatta taaaaactta aagtttcaca ttaaaccaac tggctttaaa
     2521 catactggat tattcccaga gcaagcagct aactgggatt ggatgataga gaaaataaag
     2581 aaagctaaca gacctataaa agtgttgaat ctttttgcat atacaggtgg agctactgta
     2641 gcatgtgcat ctgctggtgc agaagtatgt catgttgatg ctgcaaaagg tatggttaat
     2701 tgggcaaaag aaaatataga attatcaggt cttaaaaatc aaacagtaag atttatagtg
     2761 gatgatgttg taaagtttgt tgaaagagaa ataagaagag gtaaaaaata tgatgcaata
     2821 ataatggacc ctccttctta tggaagaggt cctaaaggag aagtatggca aatagaagaa
     2881 aagttataca gttttgtaga tttatgtatg ggagttttat cagataaccc tttatttttc
     2941 ttaattaatt cttatacaac aggattttct ccaatagttc ttgaaaatgt attgtctaca
     3001 acaataggta aaaagacaaa tacaggagat atatacggtg gggaattagg aataccagct
     3061 tccagagatg gtaagatact tccttgtggt atatttggaa gatgggagaa aaaataatga
     3121 taaaagtcat atatgaagat aaccatttat tagttgtaga aaaaccagtt aacatattgt
     3181 ctcaaggaga taatactaat gataaagata tggtaaatct tttaaaagac tatattaaaa
     3241 aaaagtataa caaacctgga aatgtatata ttggtttaat acatagattg gatagaccag
     3301 taggtggagt tatggtgttt gcaaaaacat caaaagcagc atctagattg tctgagcagg
     3361 taagaagtaa gactttcaaa aaaacttatc tagctgttgt tcatggcaag ataaaatcta
     3421 actcagatat actaaaagat tttttatata aaaataaaaa aactaatatg gtaactgtag
     3481 tgaaaaaaag ccataaagat gccaaagaag ctgaacttaa ctataatgta ttagatttta
     3541 aagaaaatct aagtttagtt caagttaatt taaagactgg tcgttctcat caaataagag
     3601 ttcaatttgc aagcagaaaa catccattat acggagacca aagatatggc aaagaaataa
     3661 ataagattgg acaacaaata tctctttggt ccaataagat agaaatttgc catcctacta
     3721 caaaagaaaa aatggaattt acatgtcatc cacctgacaa atatccatgg aatatatttt
     3781 aaatctcaaa tgaggtgatt atatgagtaa aaaattttca ataggagaaa tgtctaaact
     3841 tcataaaata agcatacaga cactaaggta ctatgaccaa atagatttat ttaaacctgt
     3901 tcatgttgac caagaaagca attacagata ttactcaatt gaccaatttt cacaattaga
     3961 tattataaaa tatttaaaat atttaggtat gcctttaaaa gaaattaagt caaagcttga
     4021 tgaaaataat gaagaaattc taaaattact aacaaataaa attaatatag tggatgaaaa
     4081 aattaaagaa ttagagctta taaagaaggt actaagcaca aagaaagaaa ctatggaaaa
     4141 aggtatgaaa gtagatgaag ttggtaaaat agtgagaaaa catattccac gaagaagtat
     4201 cttatctata gagtatgaag aaggattaag tagtgaagaa actatggaac tttccagaag
     4261 acaaatagca aatatattag aagataatat atctgtgttc tatggtggag taagtggact
     4321 tgtatctata gataaatttt taaaagaaga taaagttatt tatgacaata gttttgttac
     4381 tgttgaaaga gacttatttg atataaaatc acaaaaacat ataacagaaa tttcatctgg
     4441 tgattttatt tgtatgacat ataaagggtc ttacaaagaa aattataccg cttttaagaa
     4501 actggttgat tatgttgaat ataaaagaat acctattgaa aattatatat atgaaatacc
     4561 tataatagac cctctgtcta caaacaacga agaggaactt ctaacagaaa ttcaaataca
     4621 agttaaaaag agagaaattg acttaagatg tctagtgtag taatctagtt tacttacaca
     4681 tcttaatata gttgtctatt atcaacatat gatttttcta gatagtgatt cctaacagtg
     4741 agattttcaa aaaataggag gaaatatggc taatgctaat tccctcctat ttttaattaa
     4801 tccatatcat tttaattaag ccatgtcatc acgaagtgca tcaataatat tttctttttt
     4861 tatcttatta atagtataca acattgtaat aaatactata aataaaacac taaatatact
     4921 aattccaata ctaaaccatg gaatcacaaa atcaatatca tctgtaacca ttactttatg
     4981 aattagccat gaagaaatta ctgctatagg aagtccaaag aacaatgctc tcatgccata
     5041 aaaagcacac tcaaaattca tcatcttttg gaaatcatgc tcagacattc ctacagagcg
     5101 aagcatggca agttctctac gacgtaactt aatattagtt gaaattgtat taaacacatt
     5161 ggcaatcgaa atcagagaaa tcataattat gaaagtatag gcaaaaacat tggcaataaa
     5221 aatgtaatta cggttctcat ccatcattcc atacatattg tacagattgt actcattagt
     5281 aactccctca ctctcaataa tttgctccat ttctgcaact gactgagtag gatttttcga
     5341 ttgaaagctt aaactttcaa ttcctgtaga aacaccagga atctcaaact tatctttggc
     5401 tgaataagga gccaatactc tgaagcttgc tttcttcaca tctgaacttc tgattcctgg
     5461 aggagaatca ggtggcacta ctttgacaaa tgaaatattc acattttgtc tctcaggtat
     5521 ctccaacttg ccattagttt caggagtgag actaacattc atagaagaac tgttgaaaat
     5581 atcaataata ttattatcct ttgttaccat agaaacagca atcagttttg cattttgtcc
     5641 agtatattct tctgctggta atccaagact tttaataata ttcagataag ttctatcatc
     5701 aagaaattgc atatctactg gtaattcaag catttcactc atagaatgtg aaccctcata
     5761 gttcaaataa tcgtctgtaa gctcacttac tttggcaaca catgaatagg tcataagttt
     5821 ctgatacaaa ccttcgtaaa caccatttgc agtttttagc ttatcgtaaa gcttcaacat
     5881 ttcgttgtct ttcatacctt gtgtagaaaa aataatatcc tgatcagtaa cctctgacct
     5941 ctgatttgac atctgtttta ggtctgctac taaagcactt gtagatataa ataaaactat
     6001 gcttaaaaca agcgacaata caatacttcg ataacgtttc ttgtttcttt taaagttttt
     6061 catagcaaga actccctcaa gaccataaac tcgctgtgtt agttttgatg tcttcacaga
     6121 tttagattca atttttacct catttgtctg acgaatacac tccatcactg gtgtgttggc
     6181 agcctttctt gctggtatgt aagcagaaat taagattgta accatactta ccactgctgc
     6241 aacgatgatt gcagggatag acaatgttaa tgtcaacgga acgtttacat agagaatatt
     6301 tgcaaagttt ttagctatga cagaaatcac aagtccaata ctgactatac cgattgcaac
     6361 accaattggt ataccaatcg caccaatgca aattccttca aatagcaccg aatttcgtaa
     6421 ctgttttgct gtagctccaa cagatgaaag tattccaaat tgatgtgttc gctcgttcaa
     6481 tgacatgtta aatgaattat atattagaaa aattgagcct agcatgatta tggcaagcac
     6541 aatgccacca accgagtaca aaaacatatt gaacaaattg ttatcagaaa cacctatgaa
     6601 gcgcaacaca ttgtcattga aaatggaagc atggtcttta gtgctacttg tgtaagagcg
     6661 aatctgatgt ggatttttca atgtgacaaa aaggctaaga ctgtcagtct tgacttgttt
     6721 atcttctttg gttatcaagg tatatcctgg tgcagaagat tcttcaaata cgggctttct
     6781 acagataccc acaactgtat aagttttctt atcctgtggc acaaaaactt cactcccagc
     6841 agtgtagggg tcactctgac caagcttttc attttcactc attcgtttcc ctacatccaa
     6901 agaaatagta tcacctactg caaatttgac accaccatct gtcgcaactt tacttgaaac
     6961 aagaatctct ctactatttt caggtaatct tccagaaatt aaagttatag gcaaagtatc
     7021 aaaagcttct ttgctaaatc cagcaataaa aagatatggt ttatctggat ttctgttacc
     7081 ttcaagtgtt gcatagccaa tattttcaaa tgttgcagta tttgcaactt catcattgac
     7141 tctttgttcc tgtacaaaag aagaatccac atccaaaaac tggacatgcc aatcaccata
     7201 ttttataata gctccatttg tcatatagtt catcaaagaa actccaaagg tagtaactgc
     7261 tgtaatcata gcagctgaca gaacaactcc tataattgtt acaagagttc gagtgcgatt
     7321 ctttttcaat ccttgcagag caactttgtt aaaaatgttc attaacgatt caccttcctc
     7381 tcatcacgca ccacttttcc atctgaaata cagataattc tatctgcctg taaagcaatg
     7441 ttttcatcat gtgtgacaat aatgagagtt tggtgatatt tactatggct ctctttcagt
     7501 aactggataa tctcttgtcc atttttactg tctaagctac ctgtaggctc atcagcaagc
     7561 ataactgctg gtgcattcat caatgcacga ccaatggcaa cacgttgctg ttggccccca
     7621 gaaagttgat ttggtaaatg tgtttttcgt tcttttaaac caagtaattc taacaagtca
     7681 tttaaccgtt cttgattgac cttacgctta tccataagta taggcaatgt gatattttcc
     7741 actacattta atgtgggtat cagattgtga aactgataaa tcaatccaac ttgtcgccta
     7801 cgaaaaatag caagtttttc attgctttgg ccatatacat cttgcccctc cagatatatc
     7861 tttccacttg ttggtacgtc tactcctgca atggaatgta gtagtgtaga tttaccagaa
     7921 ccagaagaac caatgattgc agtaaactcc cctttttcaa tagtaagtga gacatggtca
     7981 agtgcagtaa ccttattttc attcttacca taaaccttac ataaatcttc aatctttaaa
     8041 aacgccatta tataagtctc ctttcatatt ttataaatat atcatagaac tttcaactta
     8101 catttaagtg acatttaaat gagagattcg tcactttgga aaacgaataa caaatattgc
     8161 accaccttgt gggtgatttt gtgcagtaat tgttcctcct tgtctcataa taatcgtctt
     8221 acaaagtgcc aatcctattc cataaccagc tgtgcttgaa tttttcccac gataaaatct
     8281 gttaaataga catggtaagt cttctttttt aaagcctgct ccgttgtcat gaatagtaag
     8341 ttgagtaaac aaaaagttgt cctcacaaac gatatcaatt tttccattat cacccacact
     8401 ctccatgcag tttttaaata tattttgaat tgcttctgaa agccaattta aatcacccaa
     8461 aatccttact ccttttggta tatcaatatg taaagtgata ttgtgcagtt ccatgggaat
     8521 caaaagttga tgaagtgcag aactaatcaa attattcaca tcaatttgtt ctttttgaaa
     8581 gactacaata cctgcatcta aacgtgataa ttttaaaagg gaagtaatca gccagtccat
     8641 tcgtataagt aattcctctg tttctcgtat caactctttt ctctcatctt catcagaagt
     8701 attctctaac agtgacaaaa taagagttac tgatgtaaga ggagtgcgaa gctggtgggc
     8761 tatgtcagct agtgagtcag caaggtgttc tttttctttt ttcagtgcgt aattttgctc
     8821 tctaatacgt aacgtcattt tagttatttc actttgtaga atagaaagct caccctcatc
     8881 tgattcagca atatacagat ggttagcatt atgaagcaca agattaattt gttctgaaat
     8941 ctgtgcaata cttttgtatc gatttttagt aaacacaaaa aatgctattc caaaggtagt
     9001 ggcagaaaca atagaaagta ttccagtcat aatactgatt gcaaatccca gggctacagt
     9061 gagaatagta attaataaga ataaaatagc aaattttcga aactctctat ttcgaaacat
     9121 aacttccccc caatctatac cctgtcccac gaacagtcaa aattatttgt ggacttgaag
     9181 ggtcatcttc tatcttctct cgcaagcgtt tgatgtacac agtcaatgtg ttgtcattaa
     9241 caaactcacc tgccgcatcc cacaattcat caagtaatct taccctcgta ataatacttt
     9301 taggattgct aataaacacc aataacaaac ggtattctaa agctgaaaga aaaacctcat
     9361 tgccattctt tttcacaaca ccggttgccg tatctacata aagcccacaa atttcaaaag
     9421 ttgacccaaa acgcccactt tttcgtagtg ctgttctaat tcgcgcaatc agttcacgtg
     9481 gacgaaaagg tttagtaata tagtcatctg cacccatatt cagtccagta acaacacttg
     9541 attcatcacc agaagccgtc aaaaagataa caggaatgtc ttgcttttct ttgatttctg
     9601 tgcaaatcgt aaacccattt ccatcaggta aagaaatatc aataagtgcc aaatcaaatt
     9661 tattcttagc aatctctaca agtgcttcac ttcttgtgga tacatgggtt actataaatc
     9721 cttctgaatt aagcaaaaga ataagatttt tggcaattgc tttatcatcc tcaactaaaa
     9781 atatccgttt catttacttt catcctcctt tattttatta gttttatata ttccagcaga
     9841 gcaatagtac actactattt tacttatcat tacacaattt tcagtagttt cttttgttct
     9901 tatataatcc atgcataata cctccattga tttattatat ttcttataac ataaatacac
     9961 aagataagac aaaaaacata atgtaagtgc aagtttttta actcactaac ttataaacat
    10021 acactcctac taacactact ttcttgatga cacatcttac aagcttatat tcatacatca
    10081 atgaaaaatt aaatttatcc atttattaaa ataaaaggca atagcttcta tagctaatgc
    10141 cctttatctt aaaatgattt aaatactata caatttttat aaatggctat aaataaactc
    10201 gtatagaata taaaatttat ctacagtctt ttgttataca aaatttataa cctcttatag
    10261 aacaaattta ccactttgta aacttatcac ttttactcag ctatattata aacttttaat
    10321 ttatctttgt ttaaatgttt cttcttaact tctactaaag cttttagcgt atccaaagat
    10381 gtcattattt cagtagaaaa ttcaatggct gttcttctca tcttaaatcc atctcttgta
    10441 gagtcatttc ctttagttgg tgtatttatt actatatcaa cttgtttatt tcttatagca
    10501 tctaatatgt ttggtctagg ctcttcaatt c
//
LOCUS       DAMJVX010000122        10404 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMJVX010000122 DAMJVX010000000
VERSION     DAMJVX010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10404
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.127"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            232..627
                     /locus_tag="PC407_002699"
     CDS             232..627
                     /locus_tag="PC407_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498131.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNNIKCEEIKDNVIEEKNNTCTTE"
     gene            670..2769
                     /locus_tag="PC407_002700"
     CDS             670..2769
                     /locus_tag="PC407_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDF2498132.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEISVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLKKDATEILVPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTGIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNHSIAVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKKDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLREYGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVSGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYY"
     gene            complement(2994..8204)
                     /locus_tag="PC407_002701"
     CDS             complement(2994..8204)
                     /locus_tag="PC407_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF2498133.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRNFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDRQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKTQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLCIPEGIYSINVSGK
                     DAEKLVGEGSIYDGMNGRNGNNAGTSGDILLSFKEIEKRGELKLYVDGGRGGNGQDGK
                     RGDTGITGEAGENAKIGSKKESKEFAKVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKASLLKGAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSAPIDDSDRNYYYFDRYVFNHASNENQVADDGEKLNALYEKHKDWTISKREDSGLV
                     GAEGKDGRSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFIGEKKNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKDELENK
                     MLSVNAKMNLLIQEIIKMKEEEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYAEKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSGSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYIDI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSESIDILKSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIC"
     gene            complement(8219..10342)
                     /locus_tag="PC407_002702"
     CDS             complement(8219..10342)
                     /locus_tag="PC407_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2498134.1"
                     /translation="MQNSVKQEPCDFSHGRFRPEADILDSRLTKVLDLFQQLNMCLHK
                     DNFRGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFEELLKDNGK
                     NIHLCKPLESDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIKEIQNNIVS
                     SLEDYVKAYTEDFISIIGSITMIQEIAKYDEVVKVFKNWLESTTTLTIERFINVLAKQ
                     SGLFLQDSCMELQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEKASKKIMEQ
                     NFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSILTNVTVTQ
                     NEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIRALIPNLVI
                     SSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILACNNLDVGSNVKI
                     NVSGKSGELVDSSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNGSKGIDGKN
                     GETGDSGSNGADAGYTSSFHGQMFKGYFAKGTSGKKGDKGGAGGNAGRGGNAGNKGNI
                     YFISDSEEQKNRIINHCDITVKDGEAGPNGRPGSGGYGGSGGYHGYNTLSFSPSGIDK
                     TVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDDGGFNAPNT
                     KLSTAVSKVEENLIKEKYQGYCSEI"
BASE COUNT         3344 a         1954 c         1227 g         3879 t
ORIGIN      
        1 tacataagct agatatataa actagatata taaactgcat atataagcta tataaaaatt
       61 atttcaacta tataaactac acatgtaaaa ttcatgtaaa tttactaata taaatcaaat
      121 aaaattacta aactctctaa actaattttg taaaacatac tctattttta acatttgtta
      181 ccttatacat aagtataaac aaaatctaac taaatgaaag gataatgtaa aatgaatatt
      241 ttaaaaatga aaaaaacagg aatattttta ggaggagtac tttttggtac tgtaggtgtt
      301 aagatacttt caagtgactg tgtaaaaaaa acttgcactc gtgcaacagc aggagcattg
      361 aaagttaaag attctgttct tgaaacaact acgaaaattc aagaaaatgt agaagatatt
      421 ttagctgaag caaaagaaat caacgagcaa gaagacttat ctacatctaa ggtaaacgtt
      481 gtacaagata ctcttaaatg cgaacaagaa gttcttaaaa ctgaaactat aaaaactgta
      541 gaagatacta attctaataa cattaaatgt gaagaaatca aagataacgt aattgaagaa
      601 aaaaataata cttgtacaac agaataaatt taatgattat tatgattaat aatatcagga
      661 ggaagtaaat tgaattttta tataaaacac gaaataagag gtcgtatacg ctttgactta
      721 caacttggca aattaaacaa caagcagtcc gatactctct tatattattt aacttctata
      781 aatggtatta ccaaagcaaa agtttacgat agaactggtg atgcagtcat ttattacact
      841 ggtgaaagag aatacataat aagagaaatc actaaattca gttttaacaa tagcaaaata
      901 gaaaacctag ttcctgaaca taccagcagg gaattaaaca attattatag agacaaatta
      961 gcaaataaac tgctagtcaa aagtatctcc aaaatatttt ttccaaagtc cttaagattt
     1021 tttttaattg gacttcattc tataaaatat tttaaaaaag gtctctcttc cctactaaat
     1081 aaaaaaatag aaatttctgt tttagatgcc actgctatag gtatttctat ttttagaaaa
     1141 gatatcaata cggctggttc agttatgttt cttcttggta tcggtgagtt attagaagaa
     1201 tggactagaa aaaaatctat agacgacttg gctcaaagta tgtcactaaa cattgaaaaa
     1261 gtatggttaa aaaaagatgc cactgaaata ctagttccaa tttcagagat aaaggaagga
     1321 gatttagtca gtgtaactat gggaaatatg attccattgg atggagttat agtatcagga
     1381 gaagcaatgg tcaatcaggc atcacttaca ggtgaatccc ttgcagttaa caaaaaagaa
     1441 ggctcttaca tatatgctgg aactggaata gagcaaggca atattgttat gtgcgtcaag
     1501 gaaaaagctg gtactactag attccagaaa atagtaacta tgattgaaga atctgaaaaa
     1561 ctaaaatctt ctgtagagag taaatttgaa catctagcag atacattagt tccatacagc
     1621 tttttaggaa gtatattaac ctatgcaatt actagaaacc ctataaaatc actctctata
     1681 ttgatggtag atttctcttg tgcattaaaa ctctctatcc ctatttcagt actatcagcc
     1741 atgagagaat gtaacaatca cagcattgct gtaaaaggtg gaaaattcct tgaaggtgtt
     1801 gcatgtgcag atactattgt atttgataag acaggcacat taacaaaagc tcaaccaact
     1861 gtttcagata taattacatt ccaaaattat aataagaagg atatgttaag acttgcagct
     1921 tgcttagaag aacacttccc acactccata gcaaatgcag tagtttatga agctgaaaaa
     1981 caagggctta gtcacaaaga aatgcataca gaggtagaat atattattgc tcatggcatt
     2041 tcaacaaagg tcgatgataa aaaggttgta ataggtagca aacattttgt atttgaagat
     2101 gagaagtgta ctatcccaga cggtgaggag gataagtaca acaatctatc agatgagtat
     2161 tctcacctat ttatggcaat atcgggtaaa ttatcagcag ttatttgcat aaatgaccct
     2221 ttacgaaaag aagcaaaata tgtaatttca aatttaagag aatatggaat aaaaaagatt
     2281 gtgatgatga ctggagatag cgaaaaaaca gctaaatcta tagcaagtaa agttggtgtt
     2341 gatgaatatt attcagaagt cttaccagaa gacaaagcca attttgttaa aagtgaaaaa
     2401 ctaaaaggtc gcaaagtaat tatgattggt gatggaatca atgattctcc tgctatttca
     2461 gaatcagatg tcggaattgc aatgagtgaa ggtgctgaaa tagcaagaga gatttcagat
     2521 attaccatat cagcagataa tttgaataat ttaatcgttt taaagcagat tagtaataag
     2581 cttatgaaaa gagttgattt aagttctaag tttattatcg gctttaattt aggtctgatt
     2641 ttacttggcg taagtggatt tgttagacca agtacatctg cctttttaca caacgcatct
     2701 actgttggaa taagtttaaa cagtatgaca aatttattgg aaaacaccaa tacttataac
     2761 tactattaat ccccattata taaaacatat gtatataaaa aatttataat tcatctttta
     2821 aaaagaaact gtcttaaaag gagctttttt ctaatcaaaa ttatataaaa catattttac
     2881 aaattttaaa ctcaacttat ttctattttt tacaaaaagg agatgattca aattttcatt
     2941 ttgaaacatc cccttttgta aatttcttta aaacttatat attaattata ttttcaacaa
     3001 ataattgtat tttcaaccaa tctttcttca tagcatttta aatcattttg ctttagataa
     3061 ttaaattcag actctacata aataccacag cctaccaatt caattgtcaa attaggtata
     3121 ttttccttta gtatactagt ctgctcataa ataattcttt ccaataaatc aacttgtaat
     3181 atagaaaaat gcttcataat ctccccatca ctatctatat ctttcttgat ttcataccaa
     3241 ctctttgaca aaattccctt caaatcaata taccctagac tcttcaatat atctatactc
     3301 tcgctccatg catacttact aggtttatca aagtatgatt ctagtttctt cttctgctca
     3361 tttgtaagtg aatcataaga aagcttctta ttaaaatgtg ccttcaaaga aatcttagcc
     3421 atagaatatg gacttctacc agagtttaat cctgcatttt tgaacttttc aattccttta
     3481 tcttctactg aagaccatat tgagtttatt gaatctgaat cattgtattt aacatcaaga
     3541 ccatgtgtaa tatctatata gttttgtgga aatacatatt gataatagac atctttttta
     3601 tcatcatata gggagaagta tccactatca tataaatgaa tatgcacctt tacttttaaa
     3661 ctatcctcta aaatatttcg tctaaggttc gggatatttg catacatggt tacatatttt
     3721 atggcatcaa agtttttatt taatggaact tcaaaactaa tttcatctcc tgtcaaaagc
     3781 tttttcactt cttcttcatt acaatcatat agagaatatg tattttcaaa tcccacacgt
     3841 gatttcccat ctaaactgcc tgttaatgct accttcaaaa tatggttatc cgttttttgc
     3901 cattggtaag catcttggtc aagaaactct ctaatctgtc tattttgctc tttcgcctga
     3961 tttataacct cgttttcatc ttctgtaata cctacatggc atctatttaa gatatttact
     4021 atattgcttc caaatggaaa cgtccatagt tgaaaagctg ctgcctcaac ctgacacaaa
     4081 tatctaacct gatttaattc aatttgatgg ataagttttt tctgatattc attcatctga
     4141 ttaccactac ctgacatttg ataaatcaat tctcccattg caagattatc ttttttctta
     4201 tcaatcttat cagtaatgat aatttgagtt tccatcatat cctgtacttt tttaaatatt
     4261 atagaaatct cctctgaatt ttccattttt aagcttccaa actttgtgct taattcagtt
     4321 aaaacttcct tcatctttaa tttacttatc tcattttcaa aaatatcttg atttttaact
     4381 gtactatcga atttttctac aaatggtata acagattctt tcaaagtttt atctttaaat
     4441 tccaccattt gagtattcaa ttcatctaat ttcttaatat tattttcttt atctttaatt
     4501 gcctctttaa ctacctctat tttactctca aaactagatt ttacattaag acaattatta
     4561 acaccactaa tcgcagtatt tataagagaa caagcagaac ttccaatact caatgcattt
     4621 ttaattttag atgtcttttg ctgtctttca atagcttgtt tacttttctc tagtgcagct
     4681 aacttatctt tttgctgttt attattgtaa tttgcctcca acatacctat ttcttttttc
     4741 tggatttttg tgtaaatttc cttatactcg ctttcactta acccctcttt tgctctttga
     4801 atatcgtcag ctttttctgc atagttatca tcatctatag acttaactat aatctcctta
     4861 atttggtcaa atttagttct tctttcttca tcaacacctg ccaaattata agtcatttta
     4921 tctaaagcat tttttctatc ttcacttagc tgttctccaa gtacaaatct cataccatca
     4981 gaatccaatt taaaatctga aaaatcaacc atcctgttag tccattggta ttcctcttct
     5041 ttagctgaaa gattttcctt caaagatttt gcactgtcaa atgcccctag acaagcttga
     5101 ccaacaccac taaatgcagt cagtaaactc aatgcgcttt gtattccaga aagaattaac
     5161 tgtgttttca tttgttcctc aagttttgcc tgtttctttt ttagttcctc tatgtcttct
     5221 tgctcctctt ctttcatctt tataatttct tgaatcaaca ggttcatctt tgcattaaca
     5281 gaaagcattt tattctcaag ttcatctttt aaaacttcta tttgttgatt tccagattca
     5341 atttgagtta ggatactttc cttataagta ttgaaatatc cttcttttaa ctcatctttc
     5401 tttccttgtt ccaattctct taaggtcttg tccagataat ctgtcaaaga acgaatatct
     5461 gtaacacgat ttcctccaat ttcactttct tcaatcatct gcacatagaa tgctgttttt
     5521 tgttcaagtg caaataaaat catactccat tcgctaaaat tcttcttttc tcctataaaa
     5581 gattctattc tatcagactg catcaattta tttataaggt attcatagat ttcctttgcc
     5641 tccttataaa attctttctt tatattcata tcatccagag aaagcactgc tttatttttc
     5701 ttctcaaaac tatagtactt ctgaagtatt tctctattta attgcatctc tattttctca
     5761 actgcatgaa aaatttcata aatatgtgaa agttctaaaa tatcagcttc taaaattccc
     5821 actgcacaca acagtctaag atttctataa cctttaattc cagaaaaacc aaatctctcg
     5881 aaaaattcat agaagaaggt atctattgac aaagaatact cctgctttaa caattccttt
     5941 tcttttaatg catctccaga gcgaccgtct tttccctctg ctccgactaa acctgaatct
     6001 tcacgtttgc taattgtcca gtccttatgt ttttcataaa gagcatttaa tttctctcca
     6061 tcatctgcta cttggttctc atttgaagca tgattaaaca catatctatc aaaataatag
     6121 taatttctat cactgtcatc tattggagca gaggtaccta tatatcttat tgtttcttca
     6181 tatatacctc taattccacc aagacctcct gcaccaccag ctcctccaga gcctcctttt
     6241 cctgctagac catgatttcc atctttttta cttatagcac cttttaataa agaagctttt
     6301 ccaaggagat aaacctgtcc agacttgctc ccaagacctg catttccacc attacctcct
     6361 gtgcctccag ttccaccttg ttttcctttt tctgctttta ccacatagta ttttgtacct
     6421 ttaaccttag caaattcttt tgactctttt ttagaaccta ttttagcatt ttccccagct
     6481 tctccagtta tccctgtatc tcctcttttt ccatcttgtc catttccacc acgtccacca
     6541 tctacgtaaa gtttaagttc tcctcttttt tctatttctt taaaagataa taaaatatca
     6601 cctgaagtac ctgcattatt gccatttctt ccattcattc cgtcgtaaat agaaccttct
     6661 ccaaccaatt tttctgcatc cttaccagat acgttgattg aataaatccc ttctggtata
     6721 cataattttt ctgtaagtag tacaatattc ttacctgaga agacttcttt ctccatatta
     6781 cagtcaattt tcaactcaca atctccatac atatatatag atgacaaaat attaagttga
     6841 taaccagata ttattttagt caaatccgat aaattcactt taggtatagg aatatatata
     6901 gaaagtacat tttctttctc atatgatttt atatatgtac aactttgaat cttctctatc
     6961 ctatactctg catcttcaag ctttttggct tcttcttccc tatattcttc ttctgctatc
     7021 caatatttga tatcttttaa tgtatcttct aaagtatttt ttagcaatga ttttaacaac
     7081 tcacttagac caacaatatg agtctcacta cagtagctat ttaactctaa aagaagacgt
     7141 gtttcttcta atatattcac tttctgtacg ccatcaccat atgaaagttc ttgtacaaac
     7201 tccataagct ctttcatatt attagtccta ctacataaat ctgtatacat cttatctaat
     7261 tgagtctttt tatcctttag actatttata gaagatagaa tggaaataga atcttccata
     7321 ccttttttga cactttctat ataaggaact aaaagttttt ctaggtcttt ataggttaca
     7381 tcaacggctt tttgtatttc tattttacaa gaagcagtaa gcatcaatcc caaatctgtt
     7441 ttttcaaatg tttcaggtag atttttcatt tcatccaaca acttttctgg ctgtctatct
     7501 agtggattac ataatttaat agatacacca tctgataatg ctacatttaa taagtacact
     7561 aattccttac aaccgtcgta atctttctct tgactatcta ggtcttcttt tgctttttcc
     7621 aatatattcc ttattttttc ttctgttaca ttaccgatta gtgttttagg tattttattg
     7681 ataagaaaga agaaattttc tttatacttt gcataatcac caagcatatc tttcaaattt
     7741 ttgcctgttt ctatcaataa tgtatgtcta gagtccagta aggattgata gtctaaaata
     7801 actaccatcc ctttcacctt tgaagatgta gaaatagttt cccttacaag catagcacca
     7861 gcaatcatat actctgctcc acggttatca aaaaaacctg ggcaatcaca taatgaaaaa
     7921 tttctatttg caatatctac aacttctgga cataaagtct tagaatatac accatttcca
     7981 atttcgacag ctgaatcttt tgcagttata tatatttgtc cagtctcttc atccgttttt
     8041 tcttccatca cgcaaccttt taaataattt atacaagtac tctttccaac gcctgaagca
     8101 cctaagaata acaaaattga cttttctttt aagtcatatt ttctaagctc gtctcttcct
     8161 tcttccaaaa ggtttacgat ttcactataa agtttatttg ccatacatcc atccctttct
     8221 atatttcgga acaatagcct tgatattttt ctttaataag attctcttct acctttgaaa
     8281 cagctgttga taacttagta tttggagcat taaatcctcc gtcgtctcct ctatctccat
     8341 attcagcgta ttctcttgct ggtcctgttt tgccttcatt ccaacttccg ccactaaatt
     8401 ctatatgata tttaattata ctatctaact tataatccca acttgtaaaa tatcctgtat
     8461 agtaaacagt cttatctata ccacttggag aaaatgaaag tgtattatat ccgtgatagc
     8521 caccacttcc tccatatcca cctgaacctg gtctaccatt tggacctgct tctccatctt
     8581 tcactgttat atcacaatga ttaattattc tatttttttg ttcctctgag tctgaaatga
     8641 agtaaatatt acctttatta ccagcatttc cacctcttcc ggcatttcca ccagctccac
     8701 ctttgtcacc ttttttacca cttgtacctt ttgcaaaata ccctttaaac atctgtccat
     8761 ggaaactact tgtatatcct gcatcagctc cattacttcc agaatctcct gtttcaccat
     8821 ttttaccatc tattccttta gaaccatttc ctccatttgc aatcagtgtt aaacgataat
     8881 ttctaatttc ttctttaata tataagtaaa tattacctgc atctctacca tcttcaccat
     8941 ttttcccata catagaacta tctacaagtt ctccgctctt tccacttaca ttaattttta
     9001 cattactacc tacatcaaga ttgttacaag caagaataat atttttctct gatgcaaagt
     9061 ccattccaag gttatcatcc atataaatag tatctttggc aaagaacata aatgtagaaa
     9121 cattgtcatc tacttttcta tttttgaaat attccaatac actactaatt actaagtttg
     9181 gaattaatgc tcttatttca atacctttct tatcattaat attaattgtt tttactataa
     9241 atggatttaa aatgtattta ccaataaatt ctctaattat ctcattacct tcttctccat
     9301 atgcacttaa tcttgttata ctgtcttcta actcttcatt ttgtgtaact gtaacatttg
     9361 tcaaaattga attaaaatca cttacactat atacataatc ctttttattt gcaacttcca
     9421 ttaatttttc tctatttggt aatgtaaata cctgtttttg tacttcataa gaaactaaaa
     9481 ctttttctaa attctttctt attataatga agttttgttc cattatcttc ttacttgcct
     9541 tttctataat aaattccata tgtacagaag gtaataaaat ttccccagtt ccatgttctg
     9601 tatatttact taaagcatca attatatcta agtttccttt tatcttattt tgtaattcca
     9661 tgcaactatc ttgtaagaat aagccacttt gtttggctaa tacgttgata aatctctcta
     9721 tagtaagagt tgttgtactt tccaaccagt ttttaaatac ttttacaacc tcatcatatt
     9781 ttgctatttc ttgaatcatt gttatacttc caataatact gataaaatct tctgtatacg
     9841 ccttaacata atcttctagg ctagatacga tattgttttg aatttctttt atcaacgtat
     9901 ctaaaactaa tcttgcatct gcactaattg gatattggaa tgctgatgtc ttatctttaa
     9961 cttcatctaa ttctagtact tgctttaata agccttccct ctcgtctgat tcaagtggct
    10021 tacaaagatg aatattttta ccattatctt ttagcaattc ttcaaataac caagcttttt
    10081 cctctatatt actatctaaa ttttcacata attgttcata ttttcttttt ataacattat
    10141 atatttgttt ttctgttctt cctaagaaac tttttgaaat gaaaaaagtt atactgcccc
    10201 taaaattgtc tttatgaaga cacatattta actgttgaaa caggtctagt acttttgtaa
    10261 gtctagaatc taatatatct gcttctggtc tgaacctccc atgactaaag tcacagggtt
    10321 cctgcttcac agaattttgc atactaaaat atgtcttact aattctccac aggccatccc
    10381 cgtagttcct acggttctta cata
//
LOCUS       DAMJVX010000123        10339 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.128, whole genome shotgun sequence.
ACCESSION   DAMJVX010000123 DAMJVX010000000
VERSION     DAMJVX010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10339
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.128"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            199..1623
                     /locus_tag="PC407_002703"
     CDS             199..1623
                     /locus_tag="PC407_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDF2498135.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDALFKRLESK"
     gene            1658..3787
                     /locus_tag="PC407_002704"
     CDS             1658..3787
                     /locus_tag="PC407_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2498136.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDK"
     gene            3817..4239
                     /locus_tag="PC407_002705"
     CDS             3817..4239
                     /locus_tag="PC407_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2498137.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4258..5409
                     /locus_tag="PC407_002706"
     CDS             4258..5409
                     /locus_tag="PC407_002706"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2498138.1"
                     /translation="MKKAIQFGAGNIGRGFIGSLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEEILNNIEKSYIFMKNL"
     regulatory      5515..5616
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5799..6371
                     /locus_tag="PC407_002707"
     CDS             5799..6371
                     /locus_tag="PC407_002707"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDF2498139.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6435..7085
                     /gene="fsa"
                     /locus_tag="PC407_002708"
     CDS             6435..7085
                     /gene="fsa"
                     /locus_tag="PC407_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2498140.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGAFLKL"
     gene            7237..10161
                     /locus_tag="PC407_002709"
     CDS             7237..10161
                     /locus_tag="PC407_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2498141.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYGTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         4241 a         1006 c         1946 g         3146 t
ORIGIN      
        1 gtattattta caaaatcata tactccaaaa tactacataa ataaaattaa acaaaaacta
       61 aaatgacaaa tgtcattatc taaagtgaca tagaaacggt ttcttaaagt tgttactata
      121 aaatataaac tctataataa aagtataaca aaagggaatc atatcgatat agaatataat
      181 tcaagaggag gtaaaattat gagtagttct aatcaatcga caataaagac atctatacag
      241 cgagtaggga aatttttaag tggtatggta atgccaaata taggagcttt tatagcatgg
      301 ggtcttataa cagcattatt tataccaaca ggatggatgc ctaatgaaaa attgagtact
      361 ataggcgacc caatgataaa atacctttta ccactattga tagcttatac tggtggtaaa
      421 gcaatagcag gacaaagagg tggcgtaata ggtgcagcag cagctatggg agtaattgtt
      481 ggtgcagata taccaatgtt tatcggagca atgataatgg gaccatttgc aggatgggta
      541 attaagaagt ttgataaatt tgtagatgga aagataccaa caggatttga aatgttagta
      601 aataactttt caataggtat aataggtatg cttcttgcaa tactaggatt ttatgcaatt
      661 ggaccagcaa tagtagcagg gacagctctt atagaatctg gagttcaatt tatagttagc
      721 aaaagtttat tgcctctagt ttcagtattt atagaacctg gaaaagtatt attcttaaac
      781 aatgcaataa atcatggtat attagggcct ataggtatag ctgaggcaaa agaagcaggt
      841 aaatctataa tgttcttact agaaagtaac cctggaccag gtttaggagt tctattagca
      901 tattggatgt ttagtagagg aagtgtaaaa caatcagcac caggagctgt aataatacat
      961 ttctttggtg gtatccatga aatttacttc ccatatattt taatgaatcc agttttaata
     1021 ttggctacaa tggcaggtgg agcagcagga atattgactt tttcaatatt aggagctggg
     1081 cttgtagcag caccatcacc aggaagtata tttgcactta tggcactagc tccaaaggga
     1141 ggattacttc cagtattagc aggagttgca gtggcaacag ttgtatcatt cttagtggca
     1201 gcaccatttg ttaagagagc ttcagcaaat caaagtgaag aagacagtac ttctttagaa
     1261 gaagctaaag caaaaatgag tgatatgaaa tctgcaagta agaactctga gaaaaatatt
     1321 gaggaaaaac agcttgaagt aaatgagatt aagaagattg tatttgcatg tgatgcagga
     1381 atgggttcga gtgcaatggg agcttccaga tttaaaaata gaataaaaaa cttagactta
     1441 aatatagaaa ttacaaattc atctgttgat aatcttccag atgatacaca aatagtagta
     1501 actcacaata cattagttga aagagttgct aagaataatt caagtgttga gatagtttca
     1561 ataaacaatt ttttaaatga cccaaattta gatgcattat ttaaaagatt agaatctaaa
     1621 taaaaataaa aagtaccact aattaggagt ggataatttg aaaaaaaaga agattacatc
     1681 taggcaaaaa aagataatac taatgatagt agaaaattca aagaaaaaca ttccaataac
     1741 tatttctgag attgcaggga ctttagagct aagttcaaga actgtactta gagatatgtc
     1801 tggaattgaa aaatggtttg atgaaaatga ttttaacttt gtaaaaaaac caggagtggg
     1861 attgatttta gaggaaaata tagaaaatca aaactttata attgagttgt tagaagaaga
     1921 aaaaatagaa aaagaataca gcaaggaaga aagaaattta attatattat caaagctact
     1981 agtttctaac gagccagtaa aatcctatta ttttgcaaaa atattaaagg tatctgaagg
     2041 ggtactgaat aatgattttg ccttagcaag taaatggttg gaaagatttg atattgagct
     2101 tgttagaaaa ccagggcttg gtgtctactt aaaaggtcaa gaaaaaaact ttagagaggc
     2161 atatgtaaat cttatatacg attcattcaa tgaaaaagaa atactagata tggtcagaaa
     2221 tataagtgaa aatatacaaa cagataaagc aatagaaatt ttaagtgaaa atagactttt
     2281 aaatttaatg gacaggtgca taataagaaa agttgaagct acattaacaa agaagctttc
     2341 agatttagat gtaaatttag ctgatagtgc atatataggg cttgtagttc atatttcatt
     2401 agccttacag aggataaaaa atggtgaaaa tataactatg gataaagagt ttctaaaaga
     2461 attatctata acagaagagt ttaaattagc taaagaaatc gtaaaaggta tggaaacaga
     2521 ttttaacatg gatataccag tagatgaggt tggatatata actatgcata taagaggtgc
     2581 aaagcaaagg tcatcttcaa accataaggc tttaaactta gatgatatag aaataatgga
     2641 aattacaaat aagatgatag atttggcgga agatgaattt aaaataagtt taaaaaatga
     2701 cgagaggtta tttaaagacc tagcaaatca cttagggcct tctataaata gattgaatat
     2761 gggattagaa ataagaaatc ctcttttaga tgaaataaaa agcaagtatt catatgccta
     2821 tgatggagtt gaaaagattt ctagaattat aaaagataaa ctaaatataa attcaatacc
     2881 tgaatcagaa ataggatata ttgcaatgca ctttgcatca gccatagaaa aaaatcttat
     2941 gatgaatact aatataaata ttgttgtggc atgtcccact ggaataggta cttcaagatt
     3001 cttatcaact aagattgaaa ataaatttcc taatttaaat atacttgaaa ccatatcagc
     3061 tataaacata gatgaagagt atttaaaaga aaaagatgtt gatttaatag tgtctacagt
     3121 tgagttaaat acaagtttaa attatatatg tgtaggtcca tttatgagtt tagatgatga
     3181 acagataatc aaggaaaaaa taaaatctat agctcaaaat aaattaatca atttaaatgc
     3241 gaaaaatgat actaaaagta agaataaagt atatgaacag ataacagaaa gtatgaatat
     3301 aggaaaagat attcttcaat ttttagagga aattagattt gaaaaatttg aaagtaaaga
     3361 tttaagtgag cttattgaag actcatcaaa aatatttgca aaatctagtg aagatataat
     3421 ctctataaaa gaatcgttaa aagaaagatt aaaaatctca attccatata tagaagaatc
     3481 aaaaattcta ttgttacatt gtatgagtga aagaatagat attatgaagt tatcaataat
     3541 aaaactagag aatagaatcg tgctagattc aaatgaagaa atcgataatg tagtttttat
     3601 gttacttcct aaaaattcac caagttatca aagacaaata atgagtgaaa taagtggttc
     3661 attaatagac aatataattt ttactaataa gataaataaa ttctctatag aagagatgac
     3721 attagaggta aaagatatag tttttaattt ttatacaaac agattaaaag catttataga
     3781 taaataaaat taatattaaa aaggaaggtt gatactatga gtaaagtatt gaatgagaat
     3841 aatatatttt tagggttaga tagtgtttca aaggaagaag ctataacttt agcaggaaga
     3901 aaattagttg aaaatggata tgtaaaagaa gaatatatac cagcaatgtt agaaagagaa
     3961 aaagtaatga ctacatatat gggaatggga gttgctattc cacatggtgt aaatgaagca
     4021 aaaaaagaaa tattatcttc tggaatagtt atattacaat tccctaatgg tatagatttt
     4081 gatggagaaa aagcatattt attaatagga atagctggtg ttggagatga acatttagaa
     4141 atactatcta atatagcagt agttttagat gatgacttaa cagaaagact aaaaaattca
     4201 aatgacaaac aagcttttat ggaagctttt gccaactaat cttaggaggg atttaaaatg
     4261 aaaaaggcga ttcagtttgg agcaggaaat attggaagag gttttattgg aagcttgtta
     4321 gttaagtcgg gttatcatgt agtatttgct gatgtaaatg aggagatatt aaactcaata
     4381 aacaaagata aaaagtatac tatacacata agagatgtag aatgtataga tgaagtaatt
     4441 gataatataa gtgcggtttc atctataaaa gaagaaataa tagatgagat tgtacaggct
     4501 gaaataataa ctacagcagt tggaccttta gttttgacta aaatagcctc tacaattgca
     4561 aaaggtataa aagctagaaa agaaaaggga ttaacaagta atctaaatat aatagcatgt
     4621 gaaaatgcaa tatacgcaag ttcttcttta aaagaagagg tacttaagta tttaaataag
     4681 gaagaagtag aatatttaga gatgtatgtt ggttttccaa actgttcagt tgatagaata
     4741 gttcctcctg gtaaaaacga aaatcctctt gatgtaacag ttgaaaattt ttatgagtgg
     4801 aatgttgaaa aacaaggatt taaaggggaa ataccaacta tagttggaat gaatttagct
     4861 gataacctta tggcttatat agaacgtaaa ttgtttacac tgaatacagg tcatgctata
     4921 acagcttata taggatattt aaaaggatat aagactatag aagagagtat aaaagataaa
     4981 tttatctgtg atatagtcaa aagtgcaatg gtagaaagtg gagaaggatt gattaaaaaa
     5041 tacaattttg actcagaagt gcactataaa tatattgata aaattttaaa tagatttaaa
     5101 aatccatatc ttaatgatga tgtattaaga gtcggaaggg aaccattaag aaaactaagt
     5161 gataaagata gattgattaa acctttaatg acagcaaaat catatggatt atcagttgac
     5221 aatttgattc ttggaatagg tgcagcgctt cattataata atagtgaaga tactcaaagt
     5281 gtagaattac aagagttgat aaagtctatt ggggtaaaaa aagccgttgc taaaatagct
     5341 aatataagca atgacgaaga aattttaaat aatatcgaaa aaagttatat atttatgaaa
     5401 aatttataaa tttacttgaa aaaaagtagt tctgataaag taactataat atattattct
     5461 acacagtttg aattatttgt tgaataatag agaattataa tatataatta aaaggaataa
     5521 attaatatat ttacctgtat atgtttgata atatggttca aatgtttcta ctaagctacc
     5581 attaatagct tgactacagg agtgaagaag atatagttta tacttatttt aaattatata
     5641 ttaagttaat tactttgaat tttaagctcc tgtctatgat atagacagga gcttttttag
     5701 tattttaata taaaatactt acaagaatag atagcaaaat tagaacaaat ataatttttt
     5761 tattaatata attttagaaa gggctaggtg gaagttcaat gaaggcttta caagaaaaga
     5821 tattgagaga aggcagtgtt tcaggaaatg atatactaaa agttgatagt tttttaaacc
     5881 atcaaattga tgtagctttt ttaaatgaaa taggaagaga atttaaagag agatttaaag
     5941 gagaaaaggt ggataagata tttacaatag aggcttcagg aatagctata gcatcaattg
     6001 tttcacagta ttttgataat gcacctgtag tatttgcaaa aaaatcagaa tctaaaaatt
     6061 tggataaaga tgtatatgaa actaatgtat attcatttac taaggcaaga gagtattcag
     6121 ttaaagtttc taaaaagtac ataaacaaag gcgaaaatat attgatagtg gatgattttt
     6181 tggcaaatgg aagagctgct cttggactta aagatttaat tgagcaagca gaagctaact
     6241 tagttggagt aggaattgtc attgaaaaag gatttcaagc tggaggagca cttttaaagg
     6301 ctaatgatgt aagattagag tctttggcag tagttgaatc tatagataat ggaactgtaa
     6361 aatttaggta atttatgata ttatataatt gtgtttagca catattttaa ctatgtaatt
     6421 atggaggatt ttttatgagg ttttttatag acacagccaa tattgaagaa ataaaagaag
     6481 caaatgattt aggtgtaata tgtggtgtaa ctactaatcc gtctctaata gctaaagaag
     6541 gtagagattt tatagaggta gttaaagaaa taagtgaaat agtagatggg ccaataagcg
     6601 cagaagtaat aagtttagaa cataaaggta tgatagagga agcagaaaaa ttatctaaga
     6661 tacataaaaa tatagttata aaaataccta tgacagcaga aggtctaaaa gcggtgaaag
     6721 tactatcaag taaaggtata aagactaatg taacgcttat attttctgct ggacaagctc
     6781 ttttagctgc tagagctgga gctacatatg taagtccttt tgttggtaga ttggatgata
     6841 tatcacaaaa tggtcttgat ttaattgaag aaatcgttga tatatttagt gtaaatagta
     6901 ttgaggctca aataatagtt gctagtgtta gaaatccaat acatgtttta caagctgcta
     6961 gaatgggagc agatatagca acagttcctc ttaaagttat aaaccaaatg attaagcatc
     7021 ctttgacaga caaaggaata gatagcttta tgaaggattg ggaaggtgct tttttaaaat
     7081 tataattata aactacgctt tataagtata ttaaagaggt tattatggaa attctaagta
     7141 ataaggctag gctattttgc ctagtcttat tttggtgtat aatatattga aacatattat
     7201 tgaaatagat taatttttta agtgaggagg cgaaaaatgg ataaaatatt aagagagata
     7261 caaagagaag ataaaaaaaa tccatatact gaccaagaac ttgctgaaat gttaaatgta
     7321 gctaggtctg aagtgatatc agttaggaaa aaaaataata ttttagattc gagagaaaga
     7381 agaaaaaaaa tattaataaa agatatatca aaaattgtaa gtgaaaatcc tcaaatgtct
     7441 gaaagaaaaa taactgaaat attaatagaa aatggatata atatatcgag aagtgcagtt
     7501 tcaaaattat taaaagagga aaatttaaat cagtcaaaga ttgggattaa ggataaagac
     7561 ccagcatcat caggaaagta tgtgaaggtg agtaaagttg tagaagaaga tggatttgaa
     7621 gaattgatag gtataaaggg aagtttgaaa gaaaaggtaa atctagctaa gtcagcaata
     7681 atgtatcctc ctaatggcct tcatactata atatatggag aaacaggagt aggtaaaagt
     7741 gaattagcta catgtatgta taaatatgct ataaaaaaca acattaaaga agagaacagc
     7801 ccatttatag tgtttaactg tgcagactat gcagaaaatc ctaatctatt gatagctcaa
     7861 ctctttgggg tagtaaaagg tgcatataca ggagcagatg caaatagaga aggtcttgta
     7921 gaacaagcta ataatggaat attgttttta gatgaaattc atagattgcc tgctgctggg
     7981 caagaaatct tattttcatt aatagataga ggagaattta gaaggttagg agaaagtagt
     8041 tctaatagaa aggcaaatgt tctcataata agtgctacaa cagaaaatcc agattctaat
     8101 ttacttcaaa catttagaag acgtatacca atggtaataa atttacctaa tatgagtgaa
     8161 agaccaaaat ctgaaagata tgaaataata ataaagtttt ttgaaagaga agcaaagcga
     8221 gtaaacaaga attttataat aactaaagaa gttatgtgtg cacttatgaa ttataagtgt
     8281 actggaaata taggtcagtt aaagagtgat atacaagtaa cttgtgctag agcttttagt
     8341 aaatcgatat tttctagcga taaggtaatt atagatttag atagtttaag ggattatata
     8401 aaaagtggat attatgattg taattgtaaa aatgaaaagt attctgagtt ttcagttgag
     8461 gatatatata tagatattag tgaattggtg agtggaaaaa atagaatttt atctgataat
     8521 gagataagcg agatatataa ttatgcagaa aaagagctta aggttttgga aagtaagtgc
     8581 ttttcagaag aagagttaaa aaatgcattt atagaaaagt tagataagaa gtttaatgct
     8641 attaaaaaca ataaaaattt atctgataga agaagtcgaa tagactgggg aggacaatta
     8701 aaagagtcta cactggagat aatggataag gtaattgtat ttattaagga gcagtttaag
     8761 catgtaaatc aaggtctgta cttggctcta gcaattcata ttgagcatgc tataggtagg
     8821 ataaaagatg gaaaaactat aataaatccg agtttagata aaataaaagc atcaatgcca
     8881 atggaatatg agttgtcaag atatataatg ggtatagtag aagatttgac acaagtaaca
     8941 tttccagaag atgagcttgg atatttggct tattatataa ataaattttg ttataatgag
     9001 gaaagtataa aagataaagt taaagtagta atagttacgc atggtaaagt tggaattgaa
     9061 atgtctaaag ttgtaaatca tattttaggt atagaatgta cactaggtat agaaatagca
     9121 cttacagatt caccatcaga ggggatagag catgtattag aagagcttca aaagatagaa
     9181 gctagaaaag gtattctagt tctaattgat atgggttctt tggtaattct tggtgatgag
     9241 gttgaaaaaa ggcttggaat tagatgtaaa actgtaaata gagtagatac attattggca
     9301 atggaagcag gaaaacttgc aaccattgaa ggaaaaagtt tagatggtat aatagctgat
     9361 ttaaagaaaa ataagaatta tgctatgata aatacaaata aatttagtta cagaaaaaac
     9421 gagtatggaa agaaaaatgt aatcattact ttatgtttga gtggtgtagg tacagcttta
     9481 aatttaaaag agcatataga aaaacaaatt aaggaatatg gcactttaat tgaagtaaag
     9541 ccagttgctt ttttaaataa taatttagaa gaagagttaa atgatattga agctgaatat
     9601 aatattattg ctatctgtgg aactattgat atagattata aaaacgtgcc ttttatttct
     9661 tataatgagg tacttggtaa aaatggtatt aataaagtat tagagcatct aaatagtaaa
     9721 caaccagctt taaatattga aaaccagttg aataatttaa tacatgaaga tttgatttta
     9781 tacaattttg aaggaatttc aaaggagtat ataattgata ctcttgtttc aaaacttgaa
     9841 gaaggtggat atgttgattc aaagtatata ttaagtgtat ataaaagaga agcaatggga
     9901 agtgttgtaa tggcctctaa ggtagccgtg ccacatggac ttccagaaaa cgttataaag
     9961 cctgcaatag caatcgcaag gttgaataaa cctatagtat gggacaataa atttatggtg
    10021 gatttggtgg tactactggc acttaaagaa aataataaga aagaaatacg aagcttattt
    10081 tcaaagatta atgaccagca tactttagaa gttttattaa atactgaaga taaaaatgaa
    10141 ataaaagaac ttttaagcta attattagta ccgagaagaa agacaaaaaa atttgcttta
    10201 gtataaaatt taaaattact caattgctaa gtagttaatt actctatata aggctagttc
    10261 tatgaaattt atttatatga ttttatagaa ttagcttttt tcttggtaaa taaaatatat
    10321 gaatttattt atgtaaaaa
//
LOCUS       DAMJVX010000124        10241 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMJVX010000124 DAMJVX010000000
VERSION     DAMJVX010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10241
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.129"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            94..2283
                     /locus_tag="PC407_002710"
     CDS             94..2283
                     /locus_tag="PC407_002710"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HDF2498142.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEEFIDIKTDLEKVTKANISIRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
     gene            2351..3118
                     /locus_tag="PC407_002711"
     CDS             2351..3118
                     /locus_tag="PC407_002711"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDF2498143.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            3176..4192
                     /locus_tag="PC407_002712"
     CDS             3176..4192
                     /locus_tag="PC407_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2498144.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVYAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            4258..4506
                     /locus_tag="PC407_002713"
     CDS             4258..4506
                     /locus_tag="PC407_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498145.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELGVLLEEKKELEEE"
     gene            complement(4683..5180)
                     /locus_tag="PC407_002714"
     CDS             complement(4683..5180)
                     /locus_tag="PC407_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2498146.1"
                     /translation="MYKIGESVMYPKEGACYISGLVTKDINHHIQKYYELTVIYNSNL
                     KISIPVLNADKIGVRPIMDENEVENFIQSLDKVDCLWVFDRKKRLKLYHDKFHSGDVF
                     EIVKLIKMLMIQDNSKQLCSTDKDFLNKAQRFALSELAAAQCKSYTIVLEEMKNHILN
                     SKNNN"
     gene            complement(5421..6485)
                     /locus_tag="PC407_002715"
     CDS             complement(5421..6485)
                     /locus_tag="PC407_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2498147.1"
                     /translation="MIRLTIANIKRYIKNHALLVNMVLFPVILMFYLNFFTGNSENQS
                     MYFSPVAIVSNSSGKYEHKLIDSSKLKENSFGLNEQDKAMDLLKNNKVSAVFILDKNF
                     SSNIDNLKRPEVKCFKTENGGGSLWAESQIESFITNSLKLKIDRNIDDKLTKTNIIDN
                     SSGKNKNSFLIVFLICYFMYINAAHLASDLFSLKKSNTLKRLVSTKHNDIKIIFSIFL
                     GLFFTQAIVYTLTLVCFTSISNFKLTLNTLMIVLANSFVATGFVFWIARVFKNEGSIS
                     LMSTFYSLIGLAISISNMIPSLDKLSFMANLSKLTPFYWTIDAINNNGNILINIIVLI
                     LIGMVFVTAGSLNLRDFARN"
     gene            complement(6488..7585)
                     /locus_tag="PC407_002716"
     CDS             complement(6488..7585)
                     /locus_tag="PC407_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2498148.1"
                     /translation="MKLYISVKTMLKVLKSSFMLNLIYFLALPLVLAGFLGMVTESIF
                     QNPIKTDLTSIVIYDKDNTKLSSNLTGYLKNDLSSILTVQKDDSKADLKLTIPKGYEN
                     NLLNEKSNNLSIEKLKSNDNIAILLQDILDTYHEKLYLNNSQKLSSEDFSKLFNKNSI
                     DTSIIKNNVKQNSYEYFALVSLGFFAIIFIMNSILSNYVSESKGLSKRLYSIPVTRVQ
                     FLIYDFIGFWIYSFIFLSLYVLFFRTLGVTFKGNFVILALLCALASYFMTSISTFITT
                     FFSKKYGTVIVYALLFLQTVFGGIFSIVNDVFTKFTSLSPTYLIGTLFSNYETFKTID
                     SIESLIFTCLIASTVLIALSIVKEKYKWREV"
     gene            complement(7599..8534)
                     /locus_tag="PC407_002717"
     CDS             complement(7599..8534)
                     /locus_tag="PC407_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498149.1"
                     /translation="MDIVRVNNITKRFNDKLALDNISFSVKEGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGDIQINGLDLSKDCIKAKSIIGLVPQELALIETLTPFDNLEYFGT
                     YYGLKGKLLQERITEALEVTGLTDVKKKKIKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKNISKKQKTTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVVSILENNSLLELGVDKKLQLTDV
                     LSVVDKNNSKITRISYEEPSLEDVFLNLTGKNLRD"
     gene            complement(8722..9366)
                     /locus_tag="PC407_002718"
     CDS             complement(8722..9366)
                     /locus_tag="PC407_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2498150.1"
                     /translation="MNKIKVLIVDDEKLIRKGLNIILSSYDDLEIVGDASNGYEALEF
                     CKTNDVDIVLMDIRMKVCDGVLGTRLIKEYDSSIALLILTTFNDDEYIEDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMYPQKTIEQKHISDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILGKLELRDRTQLVIFAFKNKIVI"
     gene            complement(9359..>10241)
                     /locus_tag="PC407_002719"
     CDS             complement(9359..>10241)
                     /locus_tag="PC407_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2498151.1"
                     /translation="FLIKEKYKYILFSLVIIFSFIIGLDKNLYLALESSSTLLIITLF
                     SVYLENENLSKLYNQSLYDKLRVSKDELKKANADLEIYANSIEELAILKERNRISREI
                     HDSVGHSLSTTIIQLNAIEKLLKDKPLISDLVHELREFVTESFQDVRRAISELKPIEY
                     ENYQNLLKIRELVNSFIKLTNINVRLTISKNTWNLSTNQSIALYRLVQESLSNASRHG
                     KATEIRIFITFNPSNLIITISDNGIGCNNIKRGNGLSSISERIYELNGKVEFDNSNTG
                     FTIRASFPKFSGGDFFE"
BASE COUNT         3892 a         1218 c         1620 g         3511 t
ORIGIN      
        1 taatatgaaa atatacaaat atataaaaaa gtttgtttag agttatagtt aatgtgtgta
       61 ttaatttaat actaatttat tggaggttta aaaatgaaat acataataaa aagagatggt
      121 acaaaagaac tttttagtaa aagtaagatt gaagatgctg tctataaagc tagtataaat
      181 agtgaaggtg gagtagatag agagttggca tatgatatat caagaaaaat agctaatgac
      241 tataattcta tggaaaaatg cttatccgta gaagaaatac aagatatagt tgaagtacta
      301 cttatggaat caaatagaaa agatatagca aaacactaca tattatatag agaaagacgc
      361 tctgaaataa ggactaaagc ttgggagttt gatgaattac aaaaatcaat atggaagaac
      421 aagtatagac ataatggtga gagttttgat gaatggataa agagaatttc aaataatagt
      481 tcaagaatat caaaactaat aagacaaaaa agatttctat ttgctggaag aattttagca
      541 aatagaggat tacagaaaga aggaaaaaaa gtaacatatt ctaattgtta tgtaatagaa
      601 ccaccaaaag atagcataga agatatattt gatactgcaa aaaaacttgc tagaactttt
      661 agttatggtg gaggagttgg aattgatatt tcaaagctaa gaccaaaagg agcgaaagtt
      721 aacaatgctg caaaacatac gactggagca gtctctttta tggaacttta cagtatgaca
      781 actggcttaa ttggtcaaag agggagaaga ggagctctta tgatttctat ggatgtaaat
      841 catccggaga tagaagagtt tatagatata aaaacagatt tggagaaggt tactaaagca
      901 aatatttcca taagggtaac agatgaattt atgcaagctg taagggaaaa aaagttatat
      961 aattgtactt ttgaagttga aactactgga gaaaaaatag ttaagatagt aaatgcttat
     1021 gatttattta ataaatttgt gaaaaataac tgggattatg cagaaccagg tatattgttt
     1081 tgggataaaa taaaaacata taattttatg agtgaagata agaacttttg cttttcagga
     1141 gtaaatcctt gtgcagaaga accacttcca agtggaggaa gttgtctact tggctcaata
     1201 aacttgtcag agtttataat acaaccattt ggtgaacatg ctacttttga tattaataaa
     1261 ttaaaatctt gcgttagaga agctgtagta gctttaaatg atgtcttaga tgaggggttg
     1321 aacttgcatc cattacaaga acaaagagat agtgtacata agtatagaca gataggttta
     1381 ggtgtaatgg gtatagctga tatgcttata aaaatgggaa taagatatgg ttctgatgaa
     1441 tctttagaac tatgtgattt aattggtaag actatgttaa atgaagctgt gaagcaatca
     1501 gcattaattg ctaaagaata tggaacattt agtgagtata aatatgaaaa tatttctaaa
     1561 tctaagtttt ttatagaaaa tctaaatgaa gacactaaag aaatggttaa aaagtatggg
     1621 cttagaaatt cacaactttt aaccatacct cctacaggtt ctatatctac aatgataggt
     1681 atcagtggtg gaatagaacc tatatttaat ctttcatata taagaaaaac tgaaagcttg
     1741 catgatgagg atgtgtatta taaagtatat actccaattg ttaaagagta tatggacaag
     1801 aaaggtatta cagatgaaga atgtttacca gatttttttg ttactgctat gaatatagga
     1861 tatgaagaaa gaataaacat gcaaaaagta tggcaaaagt atattgatgc ttctatatca
     1921 tctacaataa atataccttt tgaaactaca gttgaagatg ttttcaatat atatattaag
     1981 gcatgggagg ctggtcttaa aggagtaaca atatttagag atggatgtaa aaggagtgga
     2041 attttaataa atgaaaaaaa ggaagaaaat aataaagaag ataaaaaaga aaatagtaaa
     2101 aaagaatatt ataaaaataa taaaaaagaa aaagataata aagatgaaaa agccaaagaa
     2161 ataataaata aaaattttga agaagatgaa aaatttgtgt gccctgaatg tggaagtgaa
     2221 ggaattgcac atacaggtgg atgtagtata tgtttaaaat gtggctatag tggatgcaat
     2281 taaactctaa ttaaaaaatt ttaatatatt taaaaattaa atttttaaaa acgaaagatg
     2341 agggaatggt atgcaggata aaagtgtgag ggagattaaa gaaataatag agactttaga
     2401 agtagaaaaa tatatggaat atatagaact gttaagagtt gatgaaagaa aatctgtaca
     2461 aggtctagct ataaaactag caaaaaagct ggataatatt agaaaagaag aagaaagatt
     2521 ggaaactata aatatatttg agaacgaagg ctatgataaa ggttatttgt atattggagg
     2581 aattgatgag gcgggaagag ggccacttgc tggacctgtt gtagcttctg ttgtagtatt
     2641 taaaaaagat acaaaaatag aaggtgtaaa tgactctaag aaactgagtg aggcaaaaag
     2701 agatgaactt tttgaagtaa taaaagaaga agcactagat tatggaatag gtatagtaaa
     2761 taatgaagaa atagatgaat ttaatatatt aaatgctaca tatatggcaa tgaaaaaggc
     2821 tataaattgt ttaaaaaaag caccggatta tttattagtt gatgcagcaa caataccagg
     2881 tattgacata tctcaaaatc caattgtgaa aggagattca aaatctatat caatagcagc
     2941 agcaagtata cttgctaaag ttactaggga tagtataatg taccaatatg atagagtata
     3001 cccagaatat gggtttaaat ctcacaaagg atatggaact aaagaacact atgaagctat
     3061 tgaaaaatat ggcataactc ctatccacag aaaaagtttt ttgaaaaata tactgtaaat
     3121 ataatatgat ataatgtata taatataaat tactatagtt aatgggggat taaagatgga
     3181 atataatgaa ttattaaaaa gtgctagaga aaatttaaat ggaagttgta aagtttgtaa
     3241 ggtttgtaat ggggttgctt gtgcaggtga agtgcctggc atgggaggta aaggaagtgg
     3301 ttcttctttt atagaaaata gcaagagttt agaaaaagta aaagtaaata tgagagtaat
     3361 acatgatgta tcaaatcctg atacatctat agagatgttt ggaaagaaaa tgagtgcacc
     3421 tatatttgca gctccagtaa caggaactac tttaaatatg ggtggaaaaa ttaatgaaag
     3481 agattacata gaaccagtag ttgcaggatg tgctaatagt ggtatttatg caatggttgg
     3541 tgatacggca gtagatgctt ttttaatgga aaatttagat gtagtcaaaa agtataatgg
     3601 agctggaatt gtatttatta aaccttggga taatgaaaat attataaaaa agataagatt
     3661 agctgaagaa gctggtgcat ttgcagttgg agttgatata gatgcctgtg gtctagtaac
     3721 tttatcatta catggaaaac cagtacttcc taaaaatgtt aaacaaataa aggagttagt
     3781 aaagtctact aaactaccat ttatattgaa aggtataatg actgttgaag atgctttaat
     3841 ggcagtagaa gcaggtgtgt atgcaatagt tgtatcaaat catggaggaa gagtgcttga
     3901 ctgtacacca ggagcatgtg aagttttacc aaaaattgct gatgctgtta aaggtaaagt
     3961 tacaatactt gcagatggtg gagtgagaac tggtttagac gtactaaaaa tgataggttt
     4021 aggtgcagat gcagtattaa taggaagacc ttttgtgact gcttcatttg gtggtgcaac
     4081 tgatggtgta gaaacttatg taaataaact tcaatcagag ctaagtagtt ctatgatatt
     4141 aactggatgt caaactataa aagatattga tggaaaagtt atatacaaat aaaattttat
     4201 acaaaaaaga ggaaaaatca ttttaatgta gaataattgt acaaaggggg aatttatatg
     4261 agtagaaaag cttatgaaga agccttagta gaattagaaa aatttattga tgaaagaaaa
     4321 gaaataatta aaagtgcgga ggactgtatt gataagtaca tagttgacag aacgctacct
     4381 tttgattaca aagataaatg tgtggaatgg caacaagagt tacttgatat tgcagaggca
     4441 caagttttag aagcaaacga attaggtgtt ttactggaag agaaaaaaga acttgaggaa
     4501 gagtaagttt tattactaaa tctagggaaa atcaagaata tgatttaccc ctagattttt
     4561 ttatttaaat aaagatgtct atataattaa tctatcttta tcaatgttag attaaaaggt
     4621 ttgaagaaaa atagagtaat tgtatgaata gttaaacaat taaactagtc tataagacca
     4681 atttaattgt tattttttga atttagtata tggtttttca tttcttctaa tacaattgtg
     4741 taggatttgc attgtgctgc agccaattct gacaaagcaa acctttgagc cttatttaga
     4801 aagtccttat ctgtgctaca tagttgtttt gaattatctt gtatcataag catcttaatt
     4861 aattttacaa tctcaaaaac atctcctgaa tgaaatttat catggtatag tttaagtcgt
     4921 ttttttctat caaaaaccca taaacaatca accttatcaa gggattggat aaagttttca
     4981 acttcatttt catccataat tggacgtaca ccaatcttat cagcatttaa aactggaatc
     5041 gaaattttaa gattactatt gtaaataaca gtcaattcat agtatttctg tatatgatga
     5101 tttatatcct ttgttacaag accacttata taacaggctc cttcttttgg atacattaca
     5161 ctttcaccga ttttatacat atggttatac ctccaaactg aattttagat attgcaattt
     5221 gggtacaatg ctttaattat ctaataatta atattatatc acgaaatgct tgacaagtca
     5281 aaaagattaa tatataataa aaagtttaga ataatagata tatataattg gctataaaaa
     5341 tataagataa gaggatttaa tatattaaaa tatctagtta gtattgtata aaataacata
     5401 ctaactagat ttaataatta ttaatttcta gcaaaatctc ttaagttaag acttccggct
     5461 gttacaaata ccattccaat aagaattaaa acaattatat ttattaatat attaccatta
     5521 ttattaatag catctatagt ccaataaaat ggtgtaagct ttgaaagatt agccataaag
     5581 gataatttat caagtgatgg tatcatatta gatatggata tagctaaacc tattaaagaa
     5641 taaaacgtag acataagact tatagaacct tcgtttttaa acaccctagc tatccaaaat
     5701 acaaatccag tagcaacaaa actatttgct agtacaatca tcaatgtatt tagagtcaat
     5761 ttaaaattac ttatagatgt gaaacatact aaagttaagg tgtatacaat tgcttgagta
     5821 aaaaataacc ctaaaaatat gctgaaaatt atttttatat cattatgctt tgtagaaaca
     5881 agtcttttta aggtattaga tttctttaat gaaaataaat cagaagctag atgtgctgca
     5941 ttaatgtaca tgaaataaca tatcaagaaa actattaaaa atgaattttt attcttacct
     6001 gaactgttat caattatatt tgtcttggtc aatttatcat ctatatttct atctattttt
     6061 aattttaatg aattagtaat aaaagattct atttgtgatt ctgcccaaag actacctcca
     6121 ccattttcag ttttaaaaca tttaacctca ggtcgtttta agttatctat attgcttgaa
     6181 aagtttttat ctaaaatgaa gacagcagaa actttattat tttttaatag atccatagcc
     6241 ttgtcttgtt cgtttaatcc aaatgagttt tcttttaatt tagaactatc tattaactta
     6301 tgttcgtatt ttccagaaga attggataca atcgctactg gagaaaagta cattgattga
     6361 ttttctgagt tgccagtaaa aaagttaaga taaaacatta atataacagg aaacaaaacc
     6421 atattaacta aaagagcatg attttttata tatcttttta tgtttgcaat agtcaaccta
     6481 atcattatta aacctccctc catttatatt tttcttttac aatagaaagt gcaatcaata
     6541 ctgtagatgc aattaggcat gtaaaaatta aactttctat tgaatctata gttttaaacg
     6601 tctcataatt actgaacaat gttcctatta aataagtagg agataaactt gtaaacttag
     6661 taaacacatc atttactatg ctgaaaatac ctccaaaaac tgtttgcaag aagagtaatg
     6721 cataaactat aactgtacca tatttcttgc taaaaaaggt agttataaaa gtagatattg
     6781 aagtcataaa ataagaagct aaagcacaaa gtaaagctaa tataacaaag ttacctttga
     6841 atgttacacc gagtgttcta aaaaataaaa cgtataatga taaaaatata aatgagtaaa
     6901 tccagaaacc tataaaatca taaattaaaa attgtactct agttacaggt atagagtaga
     6961 gtctttttga aagtcctttt gattcactta cataatttga taaaatgcta ttcattataa
     7021 aaattattgc gaagaatcca agggaaacta aagcaaagta ctcataagag ttttgtttaa
     7081 cattattttt tattatagag gtatcaatag aattcttatt aaataatttt gagaaatctt
     7141 ctgaacttaa tttttgagaa ttattcagat ataatttttc atgataagta tctaatatat
     7201 cttgaagtaa tattgcaata ttatcattag atttaagttt ttctatactt agattatttg
     7261 atttttcatt taataaatta ttctcatatc ctttaggtat agtaagtttt aagtctgcct
     7321 tagaatcatc tttttgaact gttaatatag aagataaatc gttttttaga tatcctgtca
     7381 aattacttga tagctttgta ttatctttat catatataac aattgatgtc aaatctgttt
     7441 ttataggatt ttgaaatata ctttctgtca ccatcccaag aaatccagct aaaactagag
     7501 gtaaggctaa gaaatatatt aaattaagca taaaggagct ttttaaaact tttagcatag
     7561 tttttacact aatgtacaat ttcatgaaat cacctccatt agtcccttaa atttttacct
     7621 gttagattta aaaatacgtc ttctaaacta ggttcttcat aagatattcg tgttattttt
     7681 gaattatttt tgtcaacaac agataataca tctgtaagtt gtaatttttt gtctacacct
     7741 aattctaata atgaattatt ttctaaaata gatacaacac ctttagtatt ttttaaatca
     7801 aatataagtt gagcatttat attttttatt tccataatta attttgtatt tgtagatact
     7861 aatgatttta aataatcatt atctccaaaa gcaatctctt taccttcatc cataataaaa
     7921 atcttagaac aaagatgctc aacttcttcc atataatgag aagtatatat tactgtggtt
     7981 ttttgctttt tagatatatt ttttataaaa ttgaaaatat ggtttcttga ttgcgggtca
     8041 acacctacag ttggttcatc caatattaat attttaggat gatttaaaag tgctattcca
     8101 atgttcaatc tcctttgcat accaccagat aattttttga tttttttctt tttaacatca
     8161 gtgagtcctg tgacttctaa ggcttcagtt attctctctt gaagcaattt tccctttagt
     8221 ccatagtaag tgccaaagta ctctaagtta tcaaaaggag taagagtctc tataagtgct
     8281 aattcttgtg gaactaatcc tattatagac tttgctttta tacaatcttt tgacaaatct
     8341 aaaccattga tttggatgtc gccactattt ggtaacataa gatttgtaat tatatttata
     8401 agtgtagatt taccagcacc attcggtcca ataagtccaa atatctctcc ttctttaaca
     8461 gaaaagctga tattatcaag tgctaattta tcattaaatc tttttgttat attatttact
     8521 cttactatat ccataataat tcctcccaaa ataattttgt ataagttaaa aatctataat
     8581 actataataa agaattatga atcataattc tattactata agtcactaaa aaaagtgact
     8641 taagtcactt tatattttca tttattataa atgagtttgt gagtaagaaa tttctagggt
     8701 atagatttct tacctatata tttatattac aattttattt ttgaaggcaa atataactag
     8761 ttgtgtccta tcacgaagct ctagtttccc taagatattg gtgatattat tctttattgt
     8821 accttcagat aagaataatt cttggctgat ttctttgttg ttaagtccat ttgcgattaa
     8881 tctaattatg gatatttctt tttctgttaa attatacata tcagaaatat gtttttgttc
     8941 tatagttttt tgagggtaca taattttttc agccacactt ttatcaagta caatatttcc
     9001 aaaaaacgag gaacgaatcc cttcatgtaa tacttcatcc gaactatcct ttaaaagata
     9061 tcctgaagcc ccaaatttca tagcatcttc tatgtactca tcatcattaa aggttgtcaa
     9121 aattaataat gctatagagc tatcatattc ttttataagt cttgtgccta gaactccatc
     9181 acaaactttc atccgaatat ccataagaac tatgtctaca tcatttgttt tacaaaattc
     9241 taatgcttca tatccattgg aagcatctcc tacaatttct aaatcgtcat aagatgataa
     9301 aataatgtta agaccttttc taatcaattt ttcatcatct actataagaa ctttgatttt
     9361 attcaaaaaa atcacctcct gaaaatttag gaaaagacgc tctaattgtg aatccagtat
     9421 tagaattatc gaattcaact ttaccattta attcatatat tctttcgcta atactgctaa
     9481 gtccatttcc tctttttata ttgttacacc ctataccatt atcgctgata gtaattataa
     9541 gatttgaagg attaaatgtt ataaaaattc tgatttcagt tgcttttcca tgtcttgatg
     9601 cgttggaaag agattcctga acaagtctat ataaagcgat agattgattt gtagataggt
     9661 tccaggtatt ttttgatata gttaatctaa cgttgatatt tgtaagttta ataaagctat
     9721 taaccagttc tctgatttta agtaaatttt ggtaattttc atattcaatg ggttttagtt
     9781 cagatatagc tcttctcaca tcttgaaaac tttcagttac aaactctcta agttcatgaa
     9841 ctaaatcaga aatgagaggt ttgtctttta agagtttttc tatagcattt aattggatta
     9901 tcgtcgtaga aagagaatgt cccacactat cgtgtatttc cctcgatata cggtttcttt
     9961 ctttcaaaat tgctaactct tctatagaat ttgcataaat ctctaaatcg gcattagctt
    10021 tttttaattc atctttagaa accctgagtt tgtcataaag actttgattg tataatttag
    10081 ataaattctc gttttctaga tacacagaaa agagggttat aattagtaat gtagaacttg
    10141 attctagtgc taaatataaa tttttatcta aaccaataat aaaagaaaat attataacta
    10201 aagagaataa tatatattta tatttttcct ttattaaaaa t
//
LOCUS       DAMJVX010000125        10203 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMJVX010000125 DAMJVX010000000
VERSION     DAMJVX010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10203
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.130"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            77..1558
                     /locus_tag="PC407_002720"
     CDS             77..1558
                     /locus_tag="PC407_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2498152.1"
                     /translation="MKKNRLIIKNISIAFIVLFFFSVFTFFYIGNISRVLEYETNDIM
                     TVTITGWIILSFLFLCIIIYILYSKAKSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTNINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIIANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLLEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFLKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1591..5028
                     /locus_tag="PC407_002721"
     CDS             1591..5028
                     /locus_tag="PC407_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2498153.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPIFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEISKQL
                     YKNKTIELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENILGGIIITEFNDDFTIKYTNSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIIDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSEMVKTISNSIIHKDNKEVFLNTFLCKNLLSAYNKNITEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIKLKLKAERDTLTGLYN
                     KGTTENLINDFLNSDEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKHLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENTKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNNCIYQNELENNISDNNIKLANK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSER
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            5046..5792
                     /locus_tag="PC407_002722"
     CDS             5046..5792
                     /locus_tag="PC407_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDF2498154.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNSIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5741..6061)
                     /locus_tag="PC407_002723"
     CDS             complement(5741..6061)
                     /locus_tag="PC407_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2498155.1"
                     /translation="MEKIMNFLKENSTFYFATVEDNKPRVRSFGFIMDYEGKLYFGIV
                     KYKTSYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAK
                     NSLV"
     gene            6852..>10203
                     /locus_tag="PC407_002724"
     CDS             6852..>10203
                     /locus_tag="PC407_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HDF2498156.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTNPP
                     IGSTNLDGTTPNTLLITVAGSDIINQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGIGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADVEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWN
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVINLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NTSTSEAEVSSSADISVVKRSNQTVVVPG"
BASE COUNT         4145 a         1215 c         1593 g         3250 t
ORIGIN      
        1 taaaaaatac ttattaatag taaaataaag tgaatcaatt atattataat tgctaaagag
       61 agtgaggtgt agctatttga aaaaaaatag attaataata aagaatatat caatagcatt
      121 tatagtacta ttctttttta gtgtatttac attcttttat ataggtaata ttagtagagt
      181 attagaatat gaaacaaatg acataatgac agttacaatt actggatgga ttatactttc
      241 atttttattt ttatgtatta taatttacat attatattca aaagcaaaga gtcaaaaaac
      301 tatagaaaaa gtagcatata cggattttgt aacaggttat tccaactgga gaaagtttga
      361 attagatgtt acaaatttat taaagaaaac atcacaaaat aataaatatg caatggtcat
      421 attcgatatt gataaattta aagcaattaa tgatatatat ggacataaaa agggcaattt
      481 aatattaaaa gatattgcag atactttaaa tgaattgact aatatcaatg aaacttttgc
      541 tcgtgtaagt gctgataatt ttaatatatt attaacatat aacaaaaaag aggatataat
      601 aaatattatt aaaaaaatta tagcaaataa tgaacttgta aacttatctt ttggaattta
      661 tgaaatcaaa gataaggatt tatctgtaag tgtatatagt gatagagctt cacttgcaaa
      721 atcatcaata aaaaataata gtgatgtaaa ttttgcattt tttaatgata aattaagaga
      781 gaaattatta cttgaagata agatagaaaa agaaatggaa tatgctcttg aaagtgggca
      841 attcgtaatg tatttacaac caaaatataa tattaaatta gataaatttt gtggaagtga
      901 agccctagtc agatggcaat atacagaaaa agaagtcata tatccaggtg attttattcc
      961 tatatttgaa aaaaatggat ttattagaaa aattgatatg tacatattag aacaagcttg
     1021 taaagaaatc cggtctttat ttgataaagg aatttcacct ttacctatat ctgtaaactt
     1081 ctcaagagtt gattttttaa aaaaagattt tattgaaaac atagtaaata tatgtgatag
     1141 atataaaata ccatatagtt taatagaaat tgaaattact gaatctagta tgtttggaga
     1201 tactgatact ctatttaatg ttagtagaaa cctacaggat attggtttta tagttgcaat
     1261 ggatgatttt ggctcgggat attcttctgt aaatatgctt aaaaatatac cattaaatgt
     1321 aataaaatta gatagaggat tttttgttga tgataaagat gttgataaga gtcaaattgt
     1381 tataaaaagc atagtttctc tcattaagca attaggaata agggttgttg ctgaaggaat
     1441 tgaaacaaga agtcaaatag aaatgttaaa aaaagcaaat tgtgatattg ttcaaggata
     1501 ttatttttca aaaccattac caattaaaga atttgaaaaa ttagtctata aaatatagtc
     1561 agcgagtctt aaatgggagg tggtgattaa gtgataagag caaaatcgtt aaaaaatgta
     1621 ctgagtattg ttttaatatt tttatttaca ataatttctt tttctgattt tgcatttaaa
     1681 actaatgtcc aggaagaaga aaatagtaaa attaatttaa gggaaatatc cctacaaagt
     1741 tccaatgcta taaaaataaa attagatagt tctctatcct ttattaaaag tgtggcaaat
     1801 atggtttctt attttaatac tatagataac tcaaaaatag ttccattatt agctaacata
     1861 tcaaaagaat ctgactttca aagaatctct attgtatctc ctgatggaat aagtcatacc
     1921 agcgatggca aaattatcaa tgtgtcaaaa tatcagtatt ttaagaaagc tattaaagga
     1981 gaaagcaata tatctgaagt tgtgaaatct ccaattgatg gaaaagatac ttttgttata
     2041 gctactccta tatttaataa gaataacaaa ataattggtg ttttatacgg ttcttactac
     2101 aatgataaat tagctgaatt tatagatata acaagtttta atagtgaagg gtacatagat
     2161 atatttgaat ctagtggtca atttgtttta aaatcttcac atacaaacaa actgattaag
     2221 tcaagtagta acatatatga tatatttaaa aaagcttctt ttgatgatgg ttatagctat
     2281 aaaaaaatat atgaagatac aaaaaataac aagagtggct ttacctcata tgaatacaaa
     2341 ggagaaaatc gttatgctac ttatacaccc attggtgtaa atgactggta tgtaatttct
     2401 gtgataccac aatatactgt aagaagaaac attgataaaa taaatcaatt agcattttct
     2461 cttattttca aagtattctt attgtttatt atgcttataa tatatattat ttataaaaac
     2521 aataaatctc agaaagatat actaaaagct aacaaagaaa tctcatctat tacaaatagc
     2581 attcctggag gagtacaaaa gtgtaccctg ggtgaaacat gcaatattaa ttttttaagt
     2641 gatggattta tagagttgtt tggttataca agagaagaaa taaaaacatt gtttaataat
     2701 gatttttata gtttaataca taaagatgat gttgaaagaa taaaaaaaga aatttctaaa
     2761 caactttaca aaaataaaac tattgaatta gaatatagga ttataacaaa aaatggtaat
     2821 acagtatggg ttttagataa aggagaactt ataacagaac ctgatggaaa agaatatttt
     2881 tgttgtgtaa ctgtagatat tactagaaca aaacaattag aacaagagat ggaacaaaaa
     2941 aatctagaac ttgaaatgat atcagaaaat attttaggtg gtataattat aacagaattt
     3001 aatgatgatt ttactataaa atacactaac tctggttatc tttcaatgat tggatataca
     3061 cgtgaacaat tagaaaatga acttaaatac aaagcttgta atcttgtttt ttatgaagat
     3121 agaaagaagg ctcttgaaag tatacaacat caattaaaca caaaaggaat agctgtatat
     3181 caacatagaa taagaagaag agatggtaat attatctggg tttctataaa aggaaggctt
     3241 attattgatg atgatggtat tgaaaaaggt atttggattc taaatgacat aacagattta
     3301 aaaaatgcag aacaaaaact taaaataaat gaagaaagat ttagaattgc attaaagcaa
     3361 acatcaaata ctatatttga gtatgatata aaaacaaagt cacttttaaa tactagtaaa
     3421 gagcttgata aatataatat ttataaaatt actaaaaacg ttccatattc tctagtagaa
     3481 tcaggagtta ttcataaaga ttttagtgat gattttataa acatgtatga aaatgtaata
     3541 aatggagcaa aaaaagcaag ttgtgtagtt aagatgaaac ttccagatga aaattatata
     3601 tggaataaag ttaccttgac tactattttt gatgaagatg gtaatcctgt aagagcaatt
     3661 ggtatacagg aagatataag caaacaaaag gaagcagaga taagatataa tcaagaaggt
     3721 aagtatcatt cagctatatt gtcggaagca atgataacct atgaaataaa tgtatcaaaa
     3781 gatactttta tcaaaggaaa tgaaagttgg caaaaaccat ttggaattaa gcctgacaat
     3841 aattattctg aaatggtaaa aactatatca aacagtatta ttcacaaaga caataaagag
     3901 gtgtttttaa atacattttt gtgtaaaaat ttattatctg catataacaa aaatataact
     3961 gaaataaaat tagaatatcg aagaatgaat caagaaaata aaatgatttg ggtcatttgc
     4021 acaatgcact taataaaaga ttctattacg aatgatataa aagcacttgc ttatgttaaa
     4081 gatattgaca agcaaaaaaa tgaagaaatt aaattaaaat taaaagctga aagagatact
     4141 cttacaggat tatataataa aggtactaca gaaaatttga ttaatgattt cttgaactct
     4201 gacgaagcaa gcttaggaat gcatgcattt ttaattgttg atatagataa ttttaaatct
     4261 gtaaatgata atttaggaca tgtatttgga gataaagtat tatttgaagt ttctaaacac
     4321 ctaaaaccta tatttagaaa agatgatata gtaggtcgaa ttggtggaga tgaatttata
     4381 gtattcttaa aaaatattca gtcttttgag aataccaaaa agaaagcaga aaaaatatgc
     4441 gatatattta gaaattctta tacggaaaat aaaaagacat ataaaatatc tggaacagtt
     4501 ggaatttcat ttgtaccaga acatggaact gtatttgaag aactttataa aaaagcagac
     4561 atagcacttt attatgcaaa gagcaaagga aaagataagt atgcacttta taataattgt
     4621 atctaccaaa atgaacttga aaataatata agtgataata atataaaatt agcaaataaa
     4681 tgtgagaaat ccaaaccagt acttaaagaa attcttgatt ctattgataa ttatatttat
     4741 atagtgaatc caaatactta tgaacttctt tttgtcaata ataaggtgtc tcgtttggat
     4801 ttagagataa aattaggaga aacctgttat aaatcactat taaaatctga gagaccatgt
     4861 aataattgcc ctcttaacgg attagaagaa aataagcatt caaaattcaa aatagagaca
     4921 aatacatttg gtaaaaatat cagtatagca agttggatta attgcataga tggagaatac
     4981 aactgtctta tagagtgtat agattcttct ttatacaaaa ataaataaaa aaggactatg
     5041 aagatatgtt aaatagtata actttgatac ttggtataat atttacaatt tactattttt
     5101 atctcaaaat agtttttgga ggaatttcat ttagtgagat tttcttagtt gtaggaattg
     5161 tactaattat ctatcaaata tttaagaaaa aaattaaaga aaagaaggtt ttttataggg
     5221 tgttaaaaat aataatcagc atttttttaa tagtatttgt agttactgag agtttaatta
     5281 ttttttatcc taaaaatagt ttagaatcaa aaagtgatta tctcttaatt ttaggagctt
     5341 ctgtaaaaaa gactacacca agtacaaccc tgaagggtag gttagacact gcattgaaat
     5401 acctaaaaat taatgataat tgttatgtgg tagtatctgg tggtaaagga agtggagaaa
     5461 aaataactga ggcaaaagct atgaaagatt atcttataaa aaatagtata gataaaaata
     5521 ggataataga agaggataaa tccacgaata cttatgagaa ttttaaatat tcaaaattga
     5581 agatagaaga acatagtcaa agaaaactaa gtgatttaaa agtaaaaata gtaacaacag
     5641 attttcatgt tctcagaagt aaaatattag catatagaaa tggatataaa aatacgtctt
     5701 tttatgctag taaatcaaaa ctttcatttg taccaactta ctatacaaga gaattttttg
     5761 cattatggaa aacaatcatt tttgatagat aataaataaa aagactagat ataaactcat
     5821 atctagtctt ttctattccc ttaattctaa tccattttcc aactttactt gttgtagaaa
     5881 tttcaacatt gggatttaca agtaattgct tataagaagt tttatatttt acgattccaa
     5941 aatataattt tccttcataa tccattataa atccgaatga tcttactctt ggtttattat
     6001 cttcaactgt agcaaaatag aatgttgagt tttcttttaa aaaattcatt attttttcca
     6061 tgattaaatc ctctttgtat cttatactaa tataatgaat ataaataaaa tatttataat
     6121 actaatataa attgatataa aaacaattac aatattatat tgattattca aaattatata
     6181 taaaattcag aaaaacattt cagtttacat aataaaaata aagtaaacta aaattattga
     6241 ttttagatta aagtgccaga ttaagtattt aagtagattt taagcttgat ttgattagaa
     6301 aggtacaaac tatcaccgaa aaaaattaaa cccatttaaa aagcaatata aaagcttgtt
     6361 attttcgaga aaaatagacc ctctaaactc aattagataa aaataaaata caaaaaatta
     6421 tatctctgaa tatctcaagt aattaagttt aaattttacg atattttgtt tgatgattta
     6481 taatataaaa gcatattctt aatcttctca aattaaaaag agtaaaaaat aacaaaaaaa
     6541 tcactgacat ttcactgaca aattgtaatt ttgttattat aaatacattg aaaatacttg
     6601 attattgaaa aatggcataa aataatacct tttatgccat ttactattgt ttgatagttt
     6661 ttaacttatg acataagata ttttttaaca tatacaataa aatataaaaa aattgatatt
     6721 gcaaataaaa aaaatacata ttagatgtta tagaaaaaaa gcgcacttat aggcttaaaa
     6781 tgtcacataa aagagtttca aagaataata tattatgaac acaaattttg taatcacaaa
     6841 ggagtgataa tatggcaaca gtatcaggac aaatagtttt tgatagaaat agaagtgcta
     6901 ccattgattc tggggactca ggaatagcca atataccagt tgtattacaa gatatagcaa
     6961 ctggagttag acttgtggtt ctcacagatg catcaggaaa ttatgttttt actaatgttc
     7021 caaatggtaa ctatagaatt gtagaagctt ttggaactac aggaggagta ccaacaccag
     7081 ggaattttgc aacagcgata gtaggtccag ttccaattgc agaaatgcca cctattagtt
     7141 ctgtgacaaa tcctcctatt ggttcaacaa acttagatgg tacaacacct aacacactat
     7201 taataacagt agcaggttca gatataataa atcaaaatat attaaatggg cctgtgattt
     7261 atacacctat agagaatata ttggattctt gcgtgtctgt atctaatata aaccttatta
     7321 cagatgcaga taacggaaca ttcggttttt ttccaccagg tacaccaagt aatacaggac
     7381 ctgaaataga accttaccca aatgtgacgc cagattttac gtatgttgta ccagacccaa
     7441 ctaagtttac acctttagat ggagaatata cagtacaaaa tattatgaca aatgcaatga
     7501 gtaatgttat aggagcttgg tggcgtattg ctgaccatac tacaggaaat gaaacaggaa
     7561 gaatgatggt ggtaaatggt tttaatcctg gagctgtatt ttttaaatca acagtacaag
     7621 ttacacctaa cactaattat ttatttagca cttggatttt aaaccttttt aaggtaacag
     7681 gatatccacc accacagcta ggtgtaagaa tatttgacca aaataataat atactatatc
     7741 aagcaacatt aggagctcaa attccagtta atacaaacgc accagaatgg aaagaagtag
     7801 gtactgtgat taattctcaa aataataatc aacttacagt agaatttttt agtgaaggag
     7861 aaagcgccat tggaaatgac tatgctatag atgatgtttc tttaagagaa atagctgttc
     7921 caacctttac tccaataaaa acaagtaaca cagaaactgc taatgtaggt gatataatca
     7981 catatacagt tacattagat aatacttgtg atagcccttt aactaatgta ttttttaggg
     8041 atttaattcc aaatggttta gagtttatac ctggaactgt aacggttgat ggagtatctg
     8101 taccaggagt agaccctaat acaggttttc cacttccaga tattggagga ggaatagggg
     8161 ttgaagtaac ttttgatgtt gtggttgaaa gtattcctaa cccaaatcct actaataata
     8221 tagctactat agattactcc tatacacctg tagaaggcgg tataccaaat gatttttcag
     8281 tagattctaa tccagttcct gttgaagtaa tctcagcaga tgttgaagtt acaaaattat
     8341 ctgaacctac tatagtgaat ccaggagagg aattaatata cactataaaa gtagtaaata
     8401 atggaccatt cccatctgag aatgtggttt taacagatga tgtaccagct tctattataa
     8461 atccagagta ttcattagat ggaggagtaa cttttcaacc atggactgga agtttaaata
     8521 tagggacatt agaagtagga gagacaagag ttattataat taggggtatt gtaaatccat
     8581 caacagtagg aattataaca aatacagctg tagtatcttc aactacagca gacccaaacc
     8641 ttaataataa tacatctaca atagagacag aagtgaattt attagcagat attttagtaa
     8701 tgaaaacagc agaacctaat tcagctgttc caggtacttt attgagatat actattcaag
     8761 tagaaaactt aggaccagca aatgctgaaa atgtaatatt aaatgatgat attccagcct
     8821 ctattataaa tccagaatat tcattggatg gaggagcaag ttttcaacca tggaatggaa
     8881 gtcttaatat aggaacttta aactctggaa tttcattaac agttttaatt caaggaacag
     8941 ttagtttaaa ttcttcagaa tatatagtaa atacagcaac agtttcttca actacacctg
     9001 acccagattt aagcaacaat atatctacca ttattacacc agttaaccca caagcaggta
     9061 tttctattat aaaagtagct gacgaggatg tagctgttcc aggagaagaa tttgtatata
     9121 caattgaaat ttttaatgaa ggaccatcta atgctacgaa tgtagtttta acagatgata
     9181 taccagatgt tattttaaat ccagaatatt cattggatgg aggagcaact ttccaaccat
     9241 ggaatggaag tcttaatata ggtacagttg caccagggca attaataaga attatcataa
     9301 agggattagt aagttcaact gctacaggag atattactaa tacagcagaa gtaagtgcag
     9361 atgtaccaga accagttacg gattcatcaa cagtgacaac tcctatagtt ccatcagcag
     9421 atatagaagt aataaaaact agcaatatgg atacagcagt tccaggagaa acttttagtt
     9481 atactataac agttataaat ttaggaccat ctgctgcaca aagtatcgtt ttgacagatg
     9541 atataccaga tgttatttta aatccagagt attcattgga tggaggagta actttccaac
     9601 catggaatgg aaatcttagt ataggcactt tggatgctgg agaaataaga agtattataa
     9661 taagaggaac agtgagccag acagcagtag gtacaattat aaatacagca acaataagtt
     9721 cacctacacc tgacccaaat ccagataata atacttcaac agatgaaaca gatataagtc
     9781 cattagcaga tatttctgtg ataaaaggca acgaaccagt tgctatacca gggggaagat
     9841 ttatttatgg aattgaaatt gcaaatgcag gaccatcttt tgcagagaac gtaactttaa
     9901 cagataatat accagcttct attttaaatc cagagtactc aatagataat ggagtgactt
     9961 tccaaccatg gaatggaagc cttaatatag gtactttaga tgctggagag ataagaaata
    10021 tcataataag gggaacagta agtcaaacag caataggtac aattataaat acagcaacag
    10081 taagttcaac tacacctgac ccaaatctaa ataacaatac ttcaacatca gaagcagagg
    10141 taagttcatc agcagatatt tctgtagtaa aacgaagcaa tcagactgtt gttgtaccag
    10201 gag
//
LOCUS       DAMJVX010000126        10071 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMJVX010000126 DAMJVX010000000
VERSION     DAMJVX010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10071
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.131"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            90..1130
                     /locus_tag="PC407_002725"
     CDS             90..1130
                     /locus_tag="PC407_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2498157.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWEDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            1269..1721
                     /gene="tsaE"
                     /locus_tag="PC407_002726"
     CDS             1269..1721
                     /gene="tsaE"
                     /locus_tag="PC407_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDF2498158.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            1721..2437
                     /gene="tsaB"
                     /locus_tag="PC407_002727"
     CDS             1721..2437
                     /gene="tsaB"
                     /locus_tag="PC407_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDF2498159.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            2427..2903
                     /gene="rimI"
                     /locus_tag="PC407_002728"
     CDS             2427..2903
                     /gene="rimI"
                     /locus_tag="PC407_002728"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDF2498160.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKCIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            2909..3925
                     /gene="tsaD"
                     /locus_tag="PC407_002729"
     CDS             2909..3925
                     /gene="tsaD"
                     /locus_tag="PC407_002729"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDF2498161.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSNKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            4222..6930
                     /gene="hpdB"
                     /locus_tag="PC407_002730"
     CDS             4222..6930
                     /gene="hpdB"
                     /locus_tag="PC407_002730"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HDF2498162.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDHRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            6934..7191
                     /gene="hpdC"
                     /locus_tag="PC407_002731"
     CDS             6934..7191
                     /gene="hpdC"
                     /locus_tag="PC407_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HDF2498163.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDSACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            7184..8134
                     /gene="hpdA"
                     /locus_tag="PC407_002732"
     CDS             7184..8134
                     /gene="hpdA"
                     /locus_tag="PC407_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HDF2498164.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFKDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSAENISDIISFMHKNNLIEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDIDEEHLEELQDIFLDNGIACYVGHETAF"
     gene            8454..9416
                     /locus_tag="PC407_002733"
     CDS             8454..9416
                     /locus_tag="PC407_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2498165.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIITVMAAFYIFEYYLSTILMEI
                     IRKILIATGDINLKTNYFSEYANWLNDVGLQVVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(9515..>10071)
                     /locus_tag="PC407_002734"
     CDS             complement(9515..>10071)
                     /locus_tag="PC407_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2498166.1"
                     /translation="NIKLGSLLIIYFMISVIVPTSFILILQGAQPNYSGIIKFIFTPI
                     TSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKLGVIILGMCWSLWHLPLIFTLYTPEAP
                     ILGVILRSIHIVGISIFLGYLYIKTKNIWFCAIIHVLNNSAFLITNSKESTITYHDIL
                     IVSMVVLIFYVPFLFTKEYKNNLE"
BASE COUNT         3953 a         1048 c         1863 g         3207 t
ORIGIN      
        1 aaaaaataaa gttattatta aaaaactata aagactaata atcaaacttt taataataac
       61 taatttaata atgatagggg tgtatattca tgcatttatc taaaaaaata aaaaaattat
      121 ttttagatga taaagggtat tattttaata gtaattggga agatattcat attggtattt
      181 atgatacaaa attaggagat tctttaaagt atatcactaa tgaatatatt gaaaatatgg
      241 aaagggtaaa atacgattat cgtgaattaa aaaatataga aaatcaaact gatggtatgt
      301 gtattttagc aataaggcag aattacaagg cattagagta tgtaaaaaat caaaccgaag
      361 aattatgttt agaggcaata aagtataatt ataaagcact tgaatatgta aaagaacaaa
      421 cagaatactt gtgtcttgaa gctataaaaa aagattgtaa tgcacttaag tatgtacgaa
      481 ataaaacaga agggcttatt ataaaagcaa taagtcattc ttccaatata gatgttgtaa
      541 gtatactaaa ggctttagag actcaaacaa gaaggatatg tttagaagca attaaaaaag
      601 atggcaggtg tcttgcatac gttagagaac agagtgaaga attatgtata gaggccatta
      661 agcaaaatta taaagcatta aaatatgtaa aaaatcaaac ggaaaaaatg tgtatagaat
      721 ccgtaagaca aaatggtatg gcattacaat atgtaaataa acaaactgat aaaatatgta
      781 tagaagctgt caaacaagat ggaagaagtc tacaatttgt caacaataag acagaagaaa
      841 tatgtataaa tgctataaga tatttaaata aaaaatataa tattaaagat gtactaagtt
      901 acatagataa gtatacagaa gatatatgta tagagattgt aagacaaaat ggaaaaatgc
      961 ttatgtatat aaaaaatcaa acagaaaaaa tgtgtattga agcagtaaaa gagaactata
     1021 agtctcttaa gtatgtaaaa gagcaatctg agagaatatg taaagaagca ttgaagcaaa
     1081 accataaagc aaaagagtat gtaaaaattg ctattgatga ttgtatataa ggtgtagata
     1141 cagccatcaa tagtgaaaga taaaaaaaga taaagacaag tactttataa taatctaggc
     1201 ttatactata attaattaag cagtgatata aattcacatg acgattatat caaaatggag
     1261 atgaattgat ggcaaaaata tatttagaaa atgaaaataa aactagagaa ataggatata
     1321 aattaggtaa acttttaaaa gaaggcagtg ttatctgttt agtaggagat ttaggagctg
     1381 gtaaaactac aatgacacaa agcttggcag attcattagg gatagaagat tatataacaa
     1441 gtcctacttt tactataata aatgaatatg aaggaaaaat acctttgtat cattttgatg
     1501 tttatagaat aggtagtagt gatgaaatgt atgatattgg ttatgatgaa tatgtaaatt
     1561 caaatggaat ctgtataata gaatgggcta atctaataga agatatactt ccaaaagaat
     1621 atttgaatat agaattgaga tataaagatg aaggaagaga aatgatatta actccaaaag
     1681 gagagtttta taaagagata gttgaggagt tgattaaata atgaaaatac taggtatgga
     1741 tacgtcttct atggcagcaa gtgtagcagt tgttgaagat gataacttaa tatgtgagtt
     1801 tacagtaaac aacaaaaaaa ctcattcaca aaagcttatg ccaatgatag aaaatatgtt
     1861 gagtatgagt gatttaagta taaaagatat ggatttatta gctgtatgta taggtccagg
     1921 ttcgtttaca ggacttagaa taggtatggc tacagtaaaa gctatggccc atgtaaataa
     1981 tataccaata atagcagtaa attctttgga atctctagca aataacataa atttctgtga
     2041 tagaaagata tgttgtatat tagatgcgca gaaaaatcaa gtttattcag ctaaatacaa
     2101 gtttgaaaat ggagagatga tagaattaga tggtgttgat gttgtagagt ttgatgcttt
     2161 agtagatgaa attgtatcaa ctaatgaaga atttataata gttggagaag ctgtatacaa
     2221 atataaagac aagttagaga atataaaaaa tataaaaata ccttcaccag ctaataatgt
     2281 atctaacgca ggaagcttat gctctttagc attaaataaa tataataaaa atatagatgt
     2341 acatacttgt tatacgataa atcctatgta tatcagaaaa tctcaagctg aagtacaata
     2401 tgatgaaaaa atgaagaggc taaataatgg aagataatac aaaactaata gtagatgata
     2461 taaaaataga agaaatgact acagaagata tagatgaagt ttttgaagta gaaaaaaatt
     2521 gttttgaaga ttattggtca aaagagtctt ttagaaaaga gttgtctaat gaagtagcaa
     2581 ggtatatagt agctaagtta aatggaaaaa ttgtcggata tgtgggaatt tggcttatac
     2641 ttgatgaagg gcatataaat aatgtggctg ttcatagtga ctatagaggg aaaaaaatag
     2701 gggacaagtt gataaaatgc atagttgatt tatgtaaaga taataatatt gcatctatga
     2761 cattagaagt tagagcatca aataaaatag cacaaaattt atatagaaaa tatggattta
     2821 aaatgggtgg aataagaaaa gaatactaca gtgataataa agaagatgca attataatgt
     2881 ggaaccaatt aaaagaggtg tagagtttat gagtgatata ataacattag ctatagagag
     2941 tagttgtgac gaaacagctg catcagtttt aaaaaatggt agagaagtac tttcaaacat
     3001 aatctctact caaatcgaga cacataaaaa atttggtggt gtagttccag aagtggcctc
     3061 aagaaaacat gtggaaaata ttgatatagt tgttcaagaa gctttagaca aagcaaatat
     3121 aggttttaat gatatagacc atatcgcagt tacatatgga cctgggctag taggtgcttt
     3181 attggttggt ctatcttatg ctaaagcact tgcatatact ttgaatattc cacttgtagg
     3241 agtaaatcat atagaagggc atctaagtgc aaactatata gagcataagg atttaaaacc
     3301 tccttttata acattaatag tatctggagg tcatactcac ttggttgaag taaaagatta
     3361 tggcaagtat gaaattttag gaaaaacaag agatgatgca tctggagaag catttgataa
     3421 gatttcaaga gctatgaatt taggctatcc aggaggtcct attattgata atctggccaa
     3481 gaatggaaat aaacatgcta tagaatttcc aagagcctat ttagaagaag atagctatga
     3541 ttttagtttt agtggattaa aatcttctgt attaaattat ctaaatggaa aaagaatgaa
     3601 aaatgaagaa atagtagttg aagatgtagc agcatctttc caagaggctg tagtagaggt
     3661 tttatctaat aaagcactaa aagcagtaaa agacaaagga tacaatatca taactctttc
     3721 tggtggggtt gcttctaatt cggggcttag agctaaaata acagagcttg ctaaagacaa
     3781 tggtattact gttaagtatc ctcctctaat tttatgcaca gataatgcag ctatgatagg
     3841 gtgtgcagga tattataatt ttataaatgg gaaaacgcat gatatgagtt taaatgcagt
     3901 tccaaattta aagataaatc agtagagtta gatgaatttg taaatataaa agtattgttt
     3961 caaaaaagta agtagaagtg aaattaaata taataaaaat aaatatagta tatattaaaa
     4021 cttgaagtaa aactatttat aatctttata ttccaatgaa ttgcaacaaa ttttaggata
     4081 tacagattgt ctttggctta gatttgcttc aagtttaatt ttttctatag aaagattatt
     4141 ttaaaaagtg ggtaatatac cctttttttt cattcttaaa aagttttata ctaattatag
     4201 aaagattaag gaggggaaga aatgagtcaa agtaaagaag acaaaataag aagtattttg
     4261 gaagcaaaaa atattaaatc aaattttcaa aataaagaaa atttaagtga atttaatgaa
     4321 aaaaaagcaa gtaaaagagc agaagactta ttagatgtat attataatac attatctact
     4381 gctgatatgg aatttccata ttggtataat agagaatata gaaaatcaga tggtgatata
     4441 ccagtagtta gaagagctaa agctcttaaa gctgcttttt cacatatgac accaaatata
     4501 atacctggtg agaaaatagt aatgcaaaag acacgccatt atagaggttc ttttccaatg
     4561 ccatgggtaa gtgaaagttt ctttgttgct caaggagaac aaatgagaga agaggcaaag
     4621 aaattagcca gtaacacagc tgatgaactt acaaaatttg ggtctggtgg aggaaatgtt
     4681 actgaaagtt ttggaaatgt cgtttcaata gctggtaaat tcggaatgag aaaagaagaa
     4741 gtacctgtac ttgtaaaaat ggcaaaggaa tgggttggaa agtctgttga agatttaggt
     4801 tttcactatg aaaaaatgat gccagattat gatttaaaag aaaacctaat gagtacactt
     4861 atatgcatgt ttgattcagg gtatacactt cctcaaggta gagaagttat aaattatttt
     4921 tatcctttaa attatggatt agatggaatt atagaaatgg caaaagaatg taaaaaggca
     4981 gttgcaggaa atgcatctgg agatgggctt attggtatgg accgtctata tttttatgag
     5041 gctgttattc aagttattga aggattacaa acttggattt taaattatgc aaaacatgca
     5101 aaatatttag aaagtataga gacagattta gaagctaaaa aagaatactc agatttagtg
     5161 gagatattag aacatattgc acataaacaa cctcgtacat ttagggaagc tcttcagttg
     5221 acttatacaa ttcatatagc atcagtaaat gaggatgcta tatctggtat gtctatagga
     5281 cgttttggtc aaattttata tccttggtat gaacaagata tagaaaaagg actaattaca
     5341 aaagaagagg ttatcgaatt attagagctt tatagaataa aaatcacttg tattgactgt
     5401 tttgcttcag cgggcgtaaa tggaggagta ctatcaggta atacatttaa tactttatct
     5461 ataggtggat taaaagaaga tggttctaca ggagctaatg aactagaaga gttattatta
     5521 gaagcaagta tgagatgtag aactcctcaa cctagtttaa ctatgctata tgatgaaaag
     5581 cttccagaag atttcttaat gaaagctgct gaatgtacaa agcttggttc tggttatcca
     5641 gcctgggtaa acaactcaaa tggaacaaca tttatgatga aacaatttgc tgatgaaggt
     5701 atgactgttg aagaagcaag agcatttgca ttaggtggat gtttggaaac gtctccaggg
     5761 tgttggaaac agttaacttt aaacggaaaa acgtactcta ttgcaggtgg agcaggtcaa
     5821 tctgctggct ctggagttca ctttatagct aatcccaaaa ttttagaact tgttcttatg
     5881 aatggtaaag accatcgtat gaatatacaa gtatttgagc ctcataataa gccattagat
     5941 acttatgaag aagtcataga agtatttaaa gattattata aacaagcaat aaatgtttta
     6001 gaaagagcaa ataacattga gttagatata tggagaaaat ttgatacttc tataataaat
     6061 tcactactaa agccagactg tttagacaaa ggtcaacata taggaaatat gggatatcgt
     6121 tataatgcaa cattaaacgt agaaacttgt ggtactgtta ctatggtcaa ttcatttgca
     6181 gcacttaaga aacttgtata tgatgacaaa gcatttacaa tagaggaaat gaaggatgca
     6241 atattaaata attttggatt taaagatgca ttagaagtag gaaattattc tatggcagac
     6301 caagtaaaag tagataagac tggtaaatat gatgctattt acaaggcctg tcttgatgca
     6361 cctaaatatg gaaataatga tttgtatgca gataatattc ttaaaaacta tgaggtatgg
     6421 ttatcaaaag tatgtgaaga agcacaatct ttatatgcta agaagatgta cccttgccaa
     6481 atatctgttt ctacacatgg accacaggga gctgctacct tggctactcc agatggtaga
     6541 ctaagtggaa caacatattc agatggttca gtctctgcat atgcaggaac agataaaaat
     6601 ggagtatatg cattatttga atctgcaact atttgggacc aagcagtagt tcaaaattct
     6661 cagatgaatt taaaacttca tccaactact ataaaaggac aacaaggaac taaaaaatta
     6721 ttagatttaa caagaagtta tttaagaaaa ggtgggttcc atattcaata taatgttgta
     6781 gattcagaaa cattaaaaga tgcacaaaaa aatcctgaca attatcgtca attaatggta
     6841 cgtgttgctg ggtttactca atattggtgt gaattaggta aaccaataca agatgaagta
     6901 attgctagaa cagagtatga aggggtgtaa aatatgagaa agcatagtga ttgtatgaat
     6961 ttttgtgcag tagatgcaac caaaggaatt tgtagattat caaaacaaat gattaattta
     7021 gatgattcag catgtccaga gataaaagta atgccaaaat gtaaaaattg taaaaatttt
     7081 gttgaagcta atgatgaagg tataggcaaa tgtgttggcc tagagaaaga agactgggta
     7141 tattcaacat tgaatgcaat tacttgtgaa ggacatgtgt ttaatgagta gtcaaaagca
     7201 attagaaggc atgattttcg atgtacaaag cttttcagtt catgatggtc caggttgtag
     7261 aacaactgta tttttaaatg gatgtccact gagttgtaaa tggtgtgcaa atccagaaag
     7321 ttggactgta agaccacata tgatgtttag tgaattatct tgtcaatatg aaaatggatg
     7381 tactgtatgt catggtaagt gtaaaaatgg tgcattgagc tttaatcttg ataataagcc
     7441 agttatagat tggaacatct gtaaggactg tgaaagtttt gaatgcgtca actcatgtta
     7501 ttataatgcg tttaaattat gtgcaaaacc ttatactgta gatgaacttg tgcaggtaat
     7561 taaacgtgat tctaacaatt ggagaagtaa tggaggagta acatttagtg gaggtgagcc
     7621 actacttcag catgaatttt tacatgaagt attattaaag tgtcatgagg taaatataca
     7681 cactgccatt gaaacaagtg catgtgtatc aaatgaagtt tttaataaaa tatttaaaga
     7741 tattgatttt gcatttattg atataaagca tatggataga gaaaaacaca aagaacaaac
     7801 aggtgtatat aatgatttaa tattagaaaa tatatcaaac cttgcaaatt ctgactggaa
     7861 tggaaggtta gtacttagag taccagttat ctctgggttt aatgatagtg ctgaaaatat
     7921 aagtgatatt atatctttta tgcacaaaaa taatttaata gagattaact tattaccatt
     7981 tcataggcta ggagaatcaa agtggattca attaggtaag gagtatgagt attctgataa
     8041 gggagatatt gatgaagagc atcttgaaga attacaagat atatttttgg ataatggtat
     8101 agcttgctat gttggtcatg agacagcttt ttaaaatcaa atacaagttt ttttaattaa
     8161 aaaaggatgt tacactaaaa gctagagtta catccttttt tatgtggttt ttatgtagaa
     8221 ataattaagt aaagttttat atgttttata tattgttcat attgctttat gtccataaat
     8281 attactttaa atttatactt tatatatttt ttatagttta aattacagtt agagaatata
     8341 ttacgtttac tttattgaag cagaagtttt gtggtaaaat actactatga agaaatttaa
     8401 tagaatatat tttagaaaca actaaaaata atagttagga ggaagacatc tatatgaaca
     8461 aatcatggag taacttgatt attaagaata aagacaaaaa agtacgttca gggtggatta
     8521 ttataactgt tatggctgcc ttttacatat ttgagtatta tttaagcact atacttatgg
     8581 aaataataag aaaaattctg attgctacag gtgacattaa tttaaaaaca aactattttt
     8641 cggaatatgc taattggtta aatgacgttg ggttacaggt tgtatttcaa attctaacag
     8701 attcacttat gattattatt cctataattg tatggagatt tattatgaaa aatcctatta
     8761 gtgaaatggg attacattct tatagaatta agaaaaaaga aggatatgta ggaatgttat
     8821 taggaatatt taattgtaca gtaatcttta ttataacaat aacaattggt ggtggtcatg
     8881 ttacttcatg ggtaccaaaa atttcacctt taacagtttc ttggatactt gtctttattt
     8941 tagttgcttt tgcagaagaa attttaaatc gtggtttttt tatggcagtt ttacgaagat
     9001 gtaaaaatat ttattttata atgattgttc catctgttat ttttgggttg attcatatat
     9061 ggaatcctga tgttactttt ttgtctgtaa ttaatattat aattattggg attctttttt
     9121 catatatgtt tataaagtcg tcgaatatat ggatgtgtat aggttatcat tttacatgga
     9181 atgtatttca ggggattata tatggtatgc ctgtaagtgg acttcaggtt ccagggatta
     9241 taactacaca aatcactcat ggtaatctat taaatggagg gatgtttggt attgaaggtg
     9301 gaattttgac tacttttgtt aatctactta gctttatatt tgtatggtat tactatagag
     9361 attctaagta tgattttgtt agtgataaag taaatgtgac taatatcaag aattagttga
     9421 ttataataaa ttttaataat atcttctaaa cttaaagtat tctgtaggat tgttggaaat
     9481 ttacaaaaat ataaaaataa cttggatatt ctatttactc caagttattt ttatattctt
     9541 ttgtaaatag aaatggaaca taaaaaatta aaacaaccat agatactatt agtatatcat
     9601 ggtaagtgat agttgattct ttagaatttg tgataagaaa agcactatta tttaaaacat
     9661 gaattatagc acaaaaccag atattcttag tttttatata aagatatcct aaaaatattg
     9721 aaattccaac tatatgtatt gaacgtaata ttactcctaa tattggcgct tcaggtgtat
     9781 ataatgtaaa tattagtggt aagtgccaca agctccaaca catacctaat attatgactc
     9841 ctaatttttt accaaattta tcaaaaaaaa tattctgtaa aaaacctctc caggcatatt
     9901 cttcactaaa aaaaatataa atagtagtta atgatgttat tggagtaaat ataaatttta
     9961 tgataccact ataattaggt tgagcccctt gtagtattag tataaatgat gttggaacaa
    10021 ttacagaaat cataaagtaa ataattaata aagaacctag tttaatattt t
//
LOCUS       DAMJVX010000127         9896 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.132, whole genome shotgun sequence.
ACCESSION   DAMJVX010000127 DAMJVX010000000
VERSION     DAMJVX010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9896
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.132"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            164..1408
                     /locus_tag="PC407_002735"
     CDS             164..1408
                     /locus_tag="PC407_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2498167.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGIHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLINKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            1731..2828
                     /locus_tag="PC407_002736"
     CDS             1731..2828
                     /locus_tag="PC407_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2498168.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEITIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            2819..3517
                     /locus_tag="PC407_002737"
     CDS             2819..3517
                     /locus_tag="PC407_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2498169.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            3520..4584
                     /locus_tag="PC407_002738"
     CDS             3520..4584
                     /locus_tag="PC407_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2498170.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            4767..5471
                     /locus_tag="PC407_002739"
     CDS             4767..5471
                     /locus_tag="PC407_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2498171.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            5464..6786
                     /locus_tag="PC407_002740"
     CDS             5464..6786
                     /locus_tag="PC407_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2498172.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            6973..8346
                     /locus_tag="PC407_002741"
     CDS             6973..8346
                     /locus_tag="PC407_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498173.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIVMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(8719..9657)
                     /gene="pdaA"
                     /locus_tag="PC407_002742"
     CDS             complement(8719..9657)
                     /gene="pdaA"
                     /locus_tag="PC407_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF2498174.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDGGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         3672 a         1102 c         1685 g         3437 t
ORIGIN      
        1 aacttaaaaa taaagaaagt aactatgtta aatatggaaa aatggcaaaa cacttgacta
       61 ttaattggta aaggactata ataataaatt atcatattga taaaaacgaa aattatgaaa
      121 tgtctgtaaa aataaaattt aaataataac taggaggagt aatatgtttg agaacttgaa
      181 aaaagcggat cctgagattt atgaaagtat gaaaagagag ttaaaaagac aacaaagaaa
      241 tattgaatta atagcatctg aaaacatagt atcagttcca gtaatggaaa ctatgggaag
      301 tcatcttaca aataagtatg cagaaggtta ctcaggcaaa agatattatg gtggatgtga
      361 atacatagat gaagttgaaa ctcttgcaat agatagaatc aaaaaaatat ttggagcaga
      421 acatgctaat gttcaacctc actctggagc aaatgcaaat attggtgtat attttgctat
      481 gttaaaacca ggtgatgttg ttatgggaat gaatttatct caaggtggac atttaactca
      541 tggtgctcca gttaatattt ctggtcaata ttataaattt tatgaatatg gtatagataa
      601 agacagtgga cttatagatt ttgatgaagt tagaaagata gcacatgaag taaaacctaa
      661 aatgatagtt gcaggagcaa gtgcatatcc tagagaaata gattttaaaa agtttagaga
      721 gatagctgat gaggttggag cattgcttat ggtagatatg gctcatatag caggtcttgt
      781 ggctgctggt attcaccaaa atccatgtga agtggctgat tttgttacaa caacaactca
      841 caaaacttta agaggacctc gtgggggtgt tattctttgt aaagaaaaat atgctaaaga
      901 catagacaaa gcaatattcc ctggtattca aggtggacct cttgaacata taattgcttc
      961 taaggcagtt tgctttaaag aagctttaag tgatgagttt aaagaatatc aggtacaagt
     1021 tgctaaaaat gcaaaagcct tagctgaaga acttattaaa agagatttta aattgatttc
     1081 tggaggtaca gataatcatt taatactatt agatttgatt aataaaaata taactggtaa
     1141 agcagcagaa aagagattgg atgatgctta tataacagct aataaaaata caattccatt
     1201 tgatccaaat ggtgctcttg ttacaagtgg aataagactt ggtactccag ctgtaactac
     1261 tagaggtatg aaagaagaag atatggcaat aatcgctgag gctatagacc tatgtttaac
     1321 ttatgatgaa gaatctaagg ctagaacatt ggttgttggt ttaactgaaa aatatccttt
     1381 atatgaagaa tataatatta tggattaatt ataaagaaca actaaataaa atacatttaa
     1441 ataattacaa tgattgtgta tgtatttgaa tatatttaga tataaaaaag gtgtctcaaa
     1501 atgaactttt tagtttatga ggcacttttt tgtatggaat cgaacatatt tgtgttattt
     1561 aaataataaa aaatatggtt atctctattt ttagatggtc tctttgtatt ttttaattaa
     1621 aactttaaga tttcttaaga attatactag gtttttctaa agatttaaag tctatactta
     1681 taagtatagt aatcatatga aaagaaaagc tttaacgagg agggaatctt atgaaggtat
     1741 taattcttac aggaaaattt ggtatgggtc attactcagc atcaaattct ttaagtgaag
     1801 atataaaagc aaaatttgat aattcagaaa taacaattaa agatatattt gaatacatta
     1861 tgcctaacta ttcagataaa atgtacaaga ctttttctat tcttgtaaat cggggaagta
     1921 gtctttataa tttgttttat aaatgtgctg aaaatggaaa gaaagatata aaatttactt
     1981 tttcagatta ttttttaaat aaattagata cattacttca tgaagtacaa ccaacagtag
     2041 ttatttcaac ttttccattt tgttctcaat tagtatcaag atataaggaa aaatataact
     2101 caaatcttcc tttaataaca tgcataactg atataagtag tcactcagaa tggattagta
     2161 aaaatacaga ctgttattta gtagctagta aatctactaa ggaagaactt gtttttaaag
     2221 gaatagatga atctaagatt aaagttaatg gtattccagt aaaaaaagaa tttaaaagaa
     2281 tagaacatgt aaatcattca actaaaaaaa atattcttat aatgggtgga ggattaggat
     2341 tactgcctaa atcagaacaa ttttacaaag aattaaatag tttagaagga gtaaaaacta
     2401 ctgtaataac tggtaataat aaaaaaatgt actataaact atatggtaga tatgaaaata
     2461 tagaggtagt aggttacacc aatgaagtat ataaatatat gaaagattct gaccttatta
     2521 tttctaaacc aggtggaata acactatttg aaacaattta ttcagaactt ccaatattgg
     2581 catttaatcc atttttacaa caagagatag ataatgctag ttttatatta aataatgaaa
     2641 ttgggagaat acttggtaaa aataaaaaat actatgtaga tgagataaaa gatttaattt
     2701 atgatgatgc tactcttaaa gaaatgagca gtaacatgaa agaattaaaa aaacaatttg
     2761 acaataatac cttagagaat attttatttt cacttgatga acaaggagcg tgtagagaat
     2821 gtatgtagta ggtttaatta tcgttgttgc attgattttt ttagtccatt caattatacc
     2881 tacatactat aataaactat taaataaaga ggtattaaaa aatatggctg gagaaaatga
     2941 aattgcactt acatttgatg atggtccaga taagagatat acagaaaaat tactagatgt
     3001 gttaaaagaa aatgatatac aagctatgtt ttttgtagta gcaaagaatg ctgaaaaaga
     3061 gcctgaaatt ataaaaagaa tgttgagaga aaatcatata gttggtttac attcattgga
     3121 acacagaaat gcatggcttt atagttatag ttatgttaaa aaagatttta ttgaaagtac
     3181 aaatataatg aaaaatttag gtgtagatgt aaactactat cgtccaccat ggggtcatac
     3241 taatatattt tcaaattcat ttgttaaaaa atataatcta aaaatgactt tgtgggatgt
     3301 aatggcagaa gattgggaga aagatagtac agtagacatt attataaaca agttaatgag
     3361 tagaacaaaa gaaaattcta taatttgttt acatgatgca ggtgaaaatt caggtggtgc
     3421 agtaggtgca cctgagcgta ctattgaagc tttaaagata gctattccaa aacttaaagc
     3481 cagtggttta aagttcgtaa caccagaaag gatgtagtta tgataagtaa aactgaaaag
     3541 aaaaataaat gcctaggtag tgtagctttt ttagttttac taatgggcat aacaggatat
     3601 tttgttttta gagggcagtc agttgaatca ttgataaaat cattaaaagg tgcaagccct
     3661 atgtttatac taattggatt tgcaatgatg tttatatatg ttgcctgtga aggtataaat
     3721 atatacttag gtatgaaagc tttaaaccaa aaaactactc ttttaaaatg tatgggatat
     3781 gcttttattg gattttattt tagttctatt acgccttcag catcaggtgg tcaaccagca
     3841 caagtttatt atatgaaaaa agacgatata aatatatcat attcttcact tatactatta
     3901 gtcattgttg ttattcacca agtagttata ctggcatata gtggaataat gttcattatg
     3961 gaacgtgagt ttatattaaa taatgtaagt ggtatgaaca ttttactaat atatggtgta
     4021 ataacaaatg ttgctcttgt cattggagta attgcaatta tattttcaaa aaaacttgta
     4081 aacaatttta taatatcaat aacaaattta ttaggtaagt taagaataat aaaagatgtt
     4141 gaaagttcaa gaaaagttat aatatctcaa attgaagagt atgttaaagg tgctcaatat
     4201 ataaagcaga atccaaaact tgtagtccaa attttagtca taactattgt acaaataaca
     4261 gctatgtttt tagttccttt ctttgtatac aaagcttttc atctatcaac atatactgta
     4321 tttgagatat tagcgataca atccttgctt aatattgctg tttcttcatt accattgcca
     4381 ggggctgttg gagcttctga aaatagtttt atgacattat ttaagatatt tttccctggt
     4441 cacttattag ttccggcaat gttacttagt aggggaataa gtttttatgc atttgtagct
     4501 ataagtggat tgatatgtat agtagtacat gtaaaatctt ctgaaaaagt aaaagcagtg
     4561 aaaaaagttg cttatgtaag ataatattat atagattatt tttattaata tgtcaatttg
     4621 tgtgtacatt ttaattaagt tttaaacatg tttacaggag tataaactat taaaacgtgt
     4681 gacattgcta agcttttgct aataaagtat aatattttat atagtttgaa atatttaaga
     4741 aatatagcaa aaaaaggagc ttggtgatga ataaagagat tccaaaaaaa attttagtag
     4801 ctgatgataa tcctgagatt agggaaatcg tagaaatact tttaactggt gaaggttatg
     4861 aagttattat ggcaacgaat ggagaagaag ctgtaaactt agtagatagt acgattgatt
     4921 tgattatttt ggatgttgta atgcctgtaa aaacaggatt tgttgcatgt aatgaaatta
     4981 gagaaaaaac gacagcacca atattatttt taactgcaaa aactcaagac tctgataaag
     5041 ttattggatt ttcagcagga ggagatgact atttatcaaa gcctttttca tattctgaac
     5101 taatttcaag ggttaagtcg ctacttagac gttattatat ataccaggga aaggaaaagg
     5161 aaagagagcc aaatatatta aaaacaaaag agttatgtgt caatttagat actcaagatg
     5221 ttacactaaa tggagaacct gtgtttttga caactataga atatagtata ttaattctta
     5281 tgttgaaaaa tagaaaaaaa gttttttcgt ctgaaaattt atatgaaact atttgggatg
     5341 agcagtattt ttatacggca aataatacaa taatggttca tattagaaat ttaagaaaaa
     5401 agttagaaaa agaccaaaaa aatccacaat atataaaaac cgcatgggga aaggggtatt
     5461 atattgatta acaaaattaa gccatctaag ttgggagtta tactattgat atatgtagta
     5521 atctcagctg cagtggcttt cattttaaat ataccatttt actctgctgt tgaatctata
     5581 gtaaaagatg tatcagaagg gaaagtatat tttaaaagag aagtaaatca acttatggat
     5641 aattttcaaa cttatgtgaa taacaatggt ataagagcag atgatggaga tgatatagca
     5701 aaatggaata gtgataattg gtttgtttct atgaagatat ataaaggaaa taaggttttt
     5761 tatgatacat tatactatcc taatgaagta cctgataaga gtgaggaaaa tgagttttat
     5821 agcagtccca tggctagaaa ctatgagata caattttctg atattaaagc taatatagtt
     5881 gtagtagctt tatttagatt tagatttcaa aattttatag aagcaatttc tattacattt
     5941 atggcactta catttattat aacattttta ctgttgttta gtagaaagat aaagtaccta
     6001 aggaaaatag aaatgggtat aaagatagta gagagtggca gtttaaagta tagaatccca
     6061 gtaaaaggta atgatgaatt atcatcactt gcatctagta ttaatgagat gagtcataca
     6121 ttggagaatc aacttataaa agaaagggag atgaagaata aaaataaaga tatagtaact
     6181 tcgatatcac atgatgttag aactccatta acatcagtaa tttgttattt agatttgatc
     6241 aaagaccata aatacaatag tcctgagcaa ttggaacaat acataaaaaa tgtaaggtct
     6301 aaagcatatc aaataaaaga tttaacagat gaccttttta cgcattttgt taattctgga
     6361 gaggaagttt caaaagggta tgaaaaaata aatgctaatg aatttatttt tcaattactt
     6421 ttagatagtt catatacatt ggaggagaaa ggatttcata taacaataga aaatgaactt
     6481 tataaccaat atcatattta tgtagatgtt acacaattta gaaggatatt tgataattta
     6541 tgttctaata taataaaata tgctaggaaa gataagccta tagtattttt aattaaagta
     6601 gaggattcta aattactaat tatacaaaaa aatagagtaa gtaatttttc taagaatgta
     6661 gaaagttttg gaattggtat aaagaattgt gagaaaatag ttgcatatca taatggaagc
     6721 ataaatgtaa attcagatga taaagaattt gctattgaaa tatctattcc agcaaaagaa
     6781 tcataaaaat ttaaattata tattgtgtaa ttgctattat atattgtgta attgcttatg
     6841 taaaattcta tgaagcagga agcatgtgac tttagttatg ggatgttcaa tattgtgtac
     6901 ctctaggcaa gtaacaagtt tacttacaag accatataat attattatct taaaaaaagg
     6961 ggacataata atatggtttt gacaggagca cattatattt acattctatt tatggtaatt
     7021 atattaatta ctatgattat gaaaaaggat acaatagtac catgtatatt aggtgttttt
     7081 tttatgggat tgttctttga aaaaaacatc tttggtgcta taacagcagt gtttaactct
     7141 tttattatat ctttaaatga gttgggtcca attatcttga ttatagctat tatggttgca
     7201 ttatctaaag ccctagaagc caataatgct atacagtata tggtgagacc attttcgaga
     7261 gttataaaaa attctaatac agcatttttt gtaactggat ttgttatgtt agttctttct
     7321 tggtttttct ggccaactcc agctgttgcc ttagttggag ctgtattttt acctgttgct
     7381 atgagagctg ggctaccagc aattggtgtt gcagtagcat taaatttatt tggtcatggt
     7441 cttgccttgt ctacagactt tgttattcaa ggagctccaa gtataacagc tggtgcagca
     7501 ggtgttgcag tatcatatgt tataaatgat ggtatgattt tgttttgggt gatgggaatc
     7561 gtaaccatat ctatggcatt ttatacatta aaaagagata ttaacaaagg aatgtttaga
     7621 gaagaactaa aaggttttga aagtgaagaa gtagaagagt tcaatggaaa atctaaaata
     7681 gcaactattt tagtagctct gggatttctt gctgatataa ttgttatgta tatatttgac
     7741 ctaaaaggag gagatgcatc agctcttttg ggtggaacag cagtattttt aataataatt
     7801 ataaatgtta taaattttgg caaagatagt cttgaaaatg tatgtgagaa tataattgat
     7861 ggttttgtgt ttgggataaa aatatttgga gcaattatac caatagcagc atttttctat
     7921 atgggtgaag ttgcgccctt gacaggtgta tttggaaaag tattagcacc aggttcacaa
     7981 ggtttattat cagatatagg catagctctt tctcaaacaa taccacttaa taaatttgcc
     8041 gtatcaggta tagaaactgt agtgggggca ataactgggc ttgatggttc tggattttct
     8101 gggatttcat tagttggttc acttgcctct gtatttggaa ctgctataaa tgcaagtgtt
     8161 ggtgcattag ctgcattagg acaaatcagt ggtatatggg taggtggagg ctgtctagtt
     8221 ccttggggtc ttatttcagc agcggcaatt tgtggggtta gtccaataga gttggcaaag
     8281 cgaaactttg tcccagttat aaccggattg gttgtaacaa ccatagtagc aatgtttatt
     8341 atttaataaa tcgaataaat ctttaattgt acaaattaaa aatagatgtg tagctataat
     8401 ttattataat tgttttacta attttaagcc atacatctat ttttattgat atttttatta
     8461 gtattcttta ataaaataat atttttcatt atactataca taacatctaa atataacatc
     8521 taaaatactg ttatctatgt ttaatatctt gccaaatttt ttgatgtttt attaccctgt
     8581 cccaccaaca tatataagct atcgcaagtt ttttacctat actatcacca aacaaaaatt
     8641 ttaattttaa tttattattt tataatctat attatattaa ctatattagt ttgtagactt
     8701 tagatgtaat tctaaatatt aatactccaa gagttcaaat tcatatccta gttttcttgc
     8761 atttgatata aaatcattta aaatttctgt actagttttg gaaactgcat gtagtaacaa
     8821 gatagaacca tcatgtagat tatccattat tttttgcgtt gcttcttcat gtgatggttg
     8881 tttatcagta tcccagtctc tgtatgcaaa actccaaaat acagtcttgt atcctaaatt
     8941 tttagtcatt gccaagcttt tttctgagta ttttcccata ggaggtctaa aaaatttaac
     9001 catatctttc ccagttacat ctttatataa cttttcaaca tcatataatt cgtcattaaa
     9061 gttttttaga ttgcttgttt ttgttggcat agatggatgg gttttactat gatttccaac
     9121 tatatgtccc tcagaaacca ttctttttac taaatcttta ttttctttta tatatggagc
     9181 tgtaacaaaa aaaactgctt taacctggtt ttgttttaga gtgtcgagta tctttgtagt
     9241 gtatccattt tcataacctt catcaaatgt aagatatagt gttttttgac catcttttgt
     9301 aggtccattg tatagggcat catagtcaga aaatttaaat tcaatgtccg tatttacttc
     9361 tggtgtttta tgcttattgt taggtatata aaaccaatca agagtagtgt tatctaaaga
     9421 agatacatct aatttattat tatcattatt agtagtggct tttgatggtt tatctgttcc
     9481 gtctgaaact attacattgg aagtatcctt accaccatct attttttcct tatcttcttg
     9541 taactgtgtt tccttatttt gattttcgtt ttgattgttt tgtgaatttg aacatccaac
     9601 aataaaaaaa atagaaatag aaagtagtgt taaataatgc aatacttttc taaacatttt
     9661 gaagctcctt tctaaattaa taataattgc atatagtatt attataattg gaaattacta
     9721 ttaataatag ttatatcttt aaaaataaag tcacaatagt atgacaaagg gtgaataatc
     9781 aataaattta aaaaaaaatg aaaaaaatag ttgaccatat tatgagaaga tgttattata
     9841 ataaatgtgc tcaacaagaa gtaacaaaat aacaagcaca aagaagttaa ttgaat
//
LOCUS       DAMJVX010000128         9797 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMJVX010000128 DAMJVX010000000
VERSION     DAMJVX010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9797
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.133"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            61..849
                     /locus_tag="PC407_002743"
     CDS             61..849
                     /locus_tag="PC407_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2498175.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            849..1322
                     /locus_tag="PC407_002744"
     CDS             849..1322
                     /locus_tag="PC407_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2498176.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(1414..1854)
                     /locus_tag="PC407_002745"
     CDS             complement(1414..1854)
                     /locus_tag="PC407_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDF2498177.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(1869..2162)
                     /locus_tag="PC407_002746"
     CDS             complement(1869..2162)
                     /locus_tag="PC407_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDF2498178.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            complement(2205..2606)
                     /locus_tag="PC407_002747"
     CDS             complement(2205..2606)
                     /locus_tag="PC407_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498179.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            2839..4959
                     /locus_tag="PC407_002748"
     CDS             2839..4959
                     /locus_tag="PC407_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2498180.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVVDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHIARKILSINANEMIDSFVGDCIKDINFQNILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERAADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLGK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            5184..6350
                     /locus_tag="PC407_002749"
     CDS             5184..6350
                     /locus_tag="PC407_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2498181.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESVLVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIK"
     gene            6375..7817
                     /gene="nhaC"
                     /locus_tag="PC407_002750"
     CDS             6375..7817
                     /gene="nhaC"
                     /locus_tag="PC407_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2498182.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAATKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDILNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            7820..8089
                     /locus_tag="PC407_002751"
     CDS             7820..8089
                     /locus_tag="PC407_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498183.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGISINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(8165..9592)
                     /locus_tag="PC407_002752"
     CDS             complement(8165..9592)
                     /locus_tag="PC407_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HDF2498184.1"
                     /translation="MKLTKSISSMLCLALVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFLNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEIFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
BASE COUNT         3411 a         1406 c         1392 g         3588 t
ORIGIN      
        1 atattactta aaaatttgat ataatttcaa tattacgttt tttaatttgg aggtgtattt
       61 atgaatcaaa atgaggaact tgaatataag aaaaatattc taaaactagc tctatttgta
      121 ggggaactga tgataaaaaa tggagctgaa acatctaggg ttgaagacag tgtccttaga
      181 atatgcaggt caagagggtt ttatcatgta aatattttta caactcctac agtaataatt
      241 atttcagatg agaaatttga tggcttttcg tttatgaaaa cgattcaatc aagaggtata
      301 aacttaaaca agatttcttt attaaacagt ttttctcgag aatttgttag tgacaaagag
      361 tatgatattg ataatgccat tgcacgtctt tatgaaatcc aagatgtaaa accttatcct
      421 ttatggttat tttactcttg tacaggaatt gcatctgctt gttttgcttg tttgcttgga
      481 gggaattatg tattaaattt tatacttacg tttataatag caatctttgc tgcaattact
      541 tatgataaaa cgatgaagat aagtgcaata tctgcatttt cctgtttagt atcttctttt
      601 ttaattgcac tttcaggagt tctactagtt gaaataggca ttttagatga ccctaaaatg
      661 cttatagtcg gcgctataat gccactactc ccaggagttg cttttataaa agctattaga
      721 gatttaatat cagggaattt aatatcagga atttctcgtg cttttgatgc tgctatgatt
      781 attattgcaa ttgcttctgg agttggtttt gtccttgata catggtatag aataggaggt
      841 cctttctaat gactgatatg tctatgattt tgcatttttt attttcaggt gttgcaactg
      901 ctggatttgc tgttttcttt aatgctccac tatatctact tcttccagct ggagtaactg
      961 gcggaattgg ttggattgta tatgtttatt tatttaattt tactacaaat gcagtttttg
     1021 caggttttat tgctgctgct ttggtctcag cgtgtagtga gactttagca agaaaactaa
     1081 agcaaccagc tattgtgttc gtaattccag gtattcttcc tctaatacca ggaattgggc
     1141 tatataatac aatgttgtct ttgattcaga aaaattattc tttggctatg tcaaaaggta
     1201 cagatgcgct atttctaagt gctgcaatag cacttggtgt attagttgta acttcttttg
     1261 taagaacatt gaatcttttg aaaatcagaa aaaactttat tccttacaga aaatcaaaat
     1321 aaaaatataa aaatagcaat gtacttaaac tctacaaata cgtattgagt atttatttaa
     1381 aaagcacatt gctatttttt aattataatt atcttaaagg tcaattaata ttcttgttat
     1441 tactggtata aattctctac tgtctctaaa ttttttgtca ttcttcttta agtataaata
     1501 acagattatt gtaagacaaa ttccaactaa tccacttatg gcttcaactc cagttgtcat
     1561 tccaattgcc cccaatatat aataagtccc tattgttcct acaagaagtg ctataagagg
     1621 tattatataa gccattacag ctgcttttaa tacattcata ttatccatac tcacttctac
     1681 atggtcacca acttttgcac caattgtgtt gtctacttct actaatattt cttttgactc
     1741 tgctgaattt gtagtagcac attttccaca agcagcacaa gctgaatgtc tttgcatttt
     1801 taactttgca gttctatcat caacaatttc cataataaat ccttgttgat tcataactaa
     1861 ccgcctcctt attttgtaaa tttatttata gtatccataa gctcttttat catctcttca
     1921 gttttatcag catcatcatg tttaatgctt tctgctacac atccttttat atgattctct
     1981 aagataattg tgcctactct atttatagca gaccttatag cagatatttg aaccaatata
     2041 tcaacacaat aacgttcttg ttccaccatc ttttgtatcc ccttaacttg accttctatt
     2101 ctttttaatc tttttatgag agcttccctc tcattgtttg gtgctaattt tctttcttcc
     2161 ataaactcac ctcatactct aatgatgtct tatacaatta tgctctactt accaacttgt
     2221 ttaaaatata atacggttat tattactata aatgccaaaa ttgatattgc aatagaccta
     2281 ctttgattct tagtttgttc catacttaac tttccattca tcttaaatcc tgccaatggg
     2341 aataaaaata taaccagtgc atcaattcca agtacaactt gggctgcaat agtccattta
     2401 ctcaaaaagc agaatattcc gcccacacct aaaagaacct ctaatatatt tataaactta
     2461 tatgtaggtt tttttgctat atattcttca attttttcct tacattctgc acgacatgga
     2521 tatatattca tattgtcatc atattttata gctaatttat tatttggtga ctttatatcc
     2581 ctattacagt aaagacattt tttcatttta tattctcctc tagtcttcct taaaaaattt
     2641 tgcctaaatt attatacgct ctaatactat tataccaaat agatacaaaa aacaaaaata
     2701 aaaggaataa ttttagctac ttcttttact tcaatgtgta gtctataata aatttataat
     2761 aataattaaa taatattatt taatctaatt taattattat gtaatctaaa ataagtcaaa
     2821 tttgtatgag aggttaaaat gaaacaaatg gaattagcta ttgtaagttt aaaaaaagac
     2881 gctggagaaa tttatgaaaa tcaaataagg caattcttag gtgataatct aaagataaat
     2941 ttatactcat ttgaagaggg aaatttaaag ttttttaaag aaaaacttat actcctatct
     3001 gcttatttaa aatacgatga aatagtaaaa ttatctcact atgatgctca aatcattgtt
     3061 cccaagttga catttgaaaa gaacagtatt gatatgatat ccaaattaga aaaagataaa
     3121 attatttatg tatataactt aagtaaagat atggcaatag aaacaatttc tttaatacat
     3181 agattaggaa tagacaatat aaatatcctg ccctgctatc cagagattga gtttactccc
     3241 acagatgctg taatcctaac tcctggcgaa aaaatcctgc ctaagtttaa aaattgtgaa
     3301 gttgtagatt taaaatacag aataatagat ttaagttgta ttgttgaaat tgctacaaaa
     3361 accaaattaa aacatctaat aaaagatgac ctaataaaaa aatacgtaga aaaaataatt
     3421 cctacaagtt atagtacagg tgagttattg ttagatgcaa ataagtttga aagacaattt
     3481 gatttgttac tatctataat tgatgatgga attatatgca caaataatga tggaataatt
     3541 caattctata atcatatagc tagaaaaatt ttgtctataa atgcaaatga aatgattgac
     3601 tcatttgttg gtgattgtat aaaagacatc aattttcaaa acatattgac caataaaact
     3661 ccattttttg agaaattgat taaaataaat catattgata taaatttaga gattaaacat
     3721 atacaattaa atgtatttga tggatttata ctaaagatga caaagttttc tcaattagaa
     3781 aaaaagcaag ctaaattaag agcacaacta gtaaactctg gtaatatcag taaatacaca
     3841 tttgatgata tattaggttc tagtatacaa actattaata caaaaaaaat tgctaataaa
     3901 atggctcaat caaattcatc tatccttata attggtgaaa gtggtactgg taaagaatta
     3961 tttgctcaat ccattcatag tgcttctagg agaaaagatg gtccctttgt cgcagtaaat
     4021 tgtagtactt ttcaagaaaa tctactgcaa agtgaattat ttggctatga tgaaggggct
     4081 tttactggtg ctaaaaaagg tgggaaaatt ggtttatttg aattagccaa caatggaact
     4141 atatttttag acgaaattgg agaaatggat ttaaattctc aatctaaatt acttagagtc
     4201 atacaagaaa aacaagtacg acgtattggt tctaataatg tcattgatat tgatgtaaga
     4261 ataattgcag ctactaatag aaatttaaaa gaacttgtat ctaaaaatat gtttagaaga
     4321 gatttatatt ttaggttgaa tgttcttccc cttaaaatac atccacttag ggaaagagct
     4381 gctgatatat ttgaaatttt tggttcatta aaatatgata ttccatgtaa ttttattcta
     4441 agtgaggaag taaaagaaat ctttaaaatg tatagatggg aaggaaatgt aagagaatta
     4501 agaaacttag gagaatactt ctgttacttg ggaaaagata ttatagaaat atgtgattta
     4561 cctgaatata tattggacac tatagattca aattatagta gaaccgtttg taataaagta
     4621 agtgataata taaaaaagta tcaattcaac attggcaaag acaaaaacat catgaaatac
     4681 gactataatt ttaaaaggtc actagatgaa tacatattta tattggataa tttaaaaaag
     4741 gcttatgatt taaaagaaag aataggtaga aaaagcttat gtaaaatagc tttagaggaa
     4801 aatagatttt tgacagaaca acagattaga aatatgcttt tagaactaca agactttggg
     4861 cttgtagaca tacttgttgg tcgtggaggt tcaattataa catcaaaagg agtagaattt
     4921 ctaaaaaata taaataggtc taacaagtta aacagttaaa cctatttaac ctatttaatt
     4981 acattttcat atatttaaaa atttattaga aataaccttt tccacaaagt tttaagtata
     5041 attaatattt ttatcttatc tatgctctga aattacttaa tattaaatat taacttgtga
     5101 ataaatattt tttattatca atattggcac gttgtttgct taatattatt aacataaaat
     5161 taaaattact ggaggtattt acaatgaatt ataattttaa tgagattgtt gatagaagca
     5221 ataatttttc atctaaatgg tcagaaatgg aaaaaaagta tggaactaat gatttacttc
     5281 ctatgtgggt ggctgacatg gattttaagg ctgcaccttg tataattgat tccttaaaaa
     5341 atagactaga acaagagatt tatggctaca caacaagacc agattcttac aatgaatcta
     5401 tagtaaactg gctagatagg agacataact ggaaaataaa aagtgagtgg ttaatttata
     5461 gtccaggtgt tatcccagct ataagtcttt taataaatga actcacaaaa gcaaatgata
     5521 aaataatgat acaagaacca gtttacagcc cttttaatag tgttgtaaaa aataacaata
     5581 gagaactaat tataagccca ttacagaaac tagaaaatgg aaactatata atggattatg
     5641 aagacattga aaacaagatt aaagatgtaa aattattcat actttgtaat cctcataatc
     5701 cagttggtag agtatggaca aaagatgaat taaaaaaact aggagatatt tgtcttaagc
     5761 ataatgtaaa aatcatatct gatgaaatac atagtgacat tatattaaaa aaacataagc
     5821 atattcctat ggcatctatt tctaaagaat ttgaaaaaaa tacaattact tgtatggctc
     5881 caactaaaac ttttaatata gctggtcttc aatcatcata tgtaatacta cctgatgaaa
     5941 aagactataa attgttagat gatgctttta ccagaattga tataaagaga aataattgct
     6001 ttagcttagt tgcaactgaa gcttcatata ataatggtga atcttggcta gaatcatttt
     6061 tagaatattt agaatctaat atagattttg ctataaaata tataaatgaa aatatgccaa
     6121 aacttaaagt tagaaaacca gaaggtactt atttattatg ggtagacttt agtgcacttg
     6181 gattaagtga tgaggaacta gaaagtgtac ttgtacaaaa aggtaaagtt gctctaaatc
     6241 aaggaaacag ctttggcatt ggaggaagtg ggtatcaaag aataaactta gcttgtccaa
     6301 gaagtatgct tgaagaagct cttataagaa taaaaaatgc aattaaataa aaatatttaa
     6361 ggagaatttg cactatgaaa atgaatacag aaagaaaaat accttcattt agatattcct
     6421 tgttagtaat gcttgcaata atagctctaa catcagttgg tatagttgta tttaatgctt
     6481 ctataactac aatgttttta ctttcatggc ttatagtagt tcctgcagcc acgaaacttg
     6541 gttatactaa tgatgaaata gaagcttttg gatttgaagt tggtaaagat gcctttcaat
     6601 caaacttaat tatactttct gttggtgtac taatagcagc ttggatagct gctggtacaa
     6661 taccaacagt agtatatagt ggactaacaa taataacccc taaatatttt ctgcttacaa
     6721 cattaatcgt atgttcatta acttctgttg ctactggtac atcttggggg actcttggaa
     6781 cttctggtat agcaatgatg agtattggta ctagtatggg tattcctctt ggtttaacag
     6841 ctggtgcagt catttctggt gctttctttg gagataagat gtcacctcta tcagattcta
     6901 caaacttagc tgcagcagtt tgtaagacag atgtaattac acatatgaag catatgtttt
     6961 ataccacagg acctgcatac gtaatatgta tagttctgta tacagtaatt ggatttaagt
     7021 attcaaataa tactatagac tatgttcaaa taaatcaaat caaggatgta ttaaattcaa
     7081 actttcatat tggtttagta gcaatgattc caataatatt tttattatta cttttattgc
     7141 tacaaaagcc acccattata tctatactaa gttcagctat catgggactt attatagcag
     7201 tatttcaaga aggagaaaaa ataggtgata ttttaaatta catgctatca ggatttacta
     7261 tagatactgg atttgtctat gccgacaaac tattaaatcg tggtggaata atgagtatgg
     7321 ctgaaaccgt tttactagta tttgtagttt tcgttattgc cggtattctc caaaaaactg
     7381 gattcttaga agtactgctt caacctctta taactaaaat aggaaaatca agaactaaat
     7441 tggttgggtc tacttttata gtaagctact ttgcaaatgc atttagttca tcaatgatgt
     7501 ttacttctgt atttgtcgga actctaatgt ctccaatata caaagagttt aaattaaaac
     7561 cacaaaatct atctagaatt atagaagata cagctacact tggtggtcca ttaataccat
     7621 ggaattcaaa tgctgttttc tgtgctcaga ctcttggtgt aagcccacta gaatttattc
     7681 catattgttt cttaagttgg attacaccta taatttcatt tatttatggt gtaactggtt
     7741 ttactatgct tacatacaca gatgatgaaa ttgaagagtt agaaatattt gatactgctc
     7801 agagaagagt tgattgaata tgaaatttga atactattac ttaattcagg atattgcagg
     7861 tatattattg gcatttatag gacttagaat gtctattatt ggatttagga tactttctat
     7921 gaaggggatt tctataaata cacttttaat tgtaattaaa tactgcttat ttaccatagc
     7981 aggactgaat ttattaatat ctaagtttgg aattagacac tggatttgga gtgtttgtat
     8041 gttgataata tccataataa taaatcccag gattaaggta tcaaaataga aatagttatt
     8101 agacttaaat ttttataaaa aaactgcttt acaaattaaa gcagtttttt attgttactt
     8161 ataattaaat aactggtgta tcagataagt aatcattact tacataacca tatccaccat
     8221 tatactttat ttttacccat ccagtagcaa atatttctat tgtttctact ttgcttccat
     8281 atgaaaggtg acctattttt tcatcttctg tacttggtcc tttcctaaca tttagaccat
     8341 ctttttcttt tacatacttt atttttatat tttcattact attatcaggc ttattatttg
     8401 tcaaataaga cccactaaca tatccaatac cttcattgta ttttattttt acccatccag
     8461 tttttaacac atctaaaact tctacttcat caccattatt tagatatcct ataacagaag
     8521 cttctattgt agcatcattt cttatattta ccttatctgt attagttaca tacattactc
     8581 tcttattgtt actatttgcc tgtaaatctt ttaattcttt aaatgcattt tcatttcctc
     8641 cactaccaac ttgagttata gaggtagcat ttttattcaa aaatacactc ttttgtgttg
     8701 cacttaattg ttctcctacg attagtacag gagaattttc ttttgatgcc agtactccta
     8761 ctgctagagc gtctataagt tgattttgat ttttcattcc atccttagcc acaaatacat
     8821 tgtttaattt attagatgta taaaactttt ctattacttt accattagtt tcatttctat
     8881 ctgctccacc tattctttct gaattaggta aactttgttt tattttatct gatacagcat
     8941 tagtagagcc tattatgtat gatttattta tattcttaga ttctataaaa tctttaaagc
     9001 tagatatacc agatgcatca gatactggaa gtataaccat tccattagta gcagctacag
     9061 aagctatact aactgcatct gcaagacctg tgtacccatt tactacagct atttctgata
     9121 tatcttttaa tccttctatt tttttagcta tactcaaagc tgtttggtat ctatcactac
     9181 caccaactct atcagtagtt aaattattag attctaactg tttttgcaca tcatttgata
     9241 cagaactaga tagaccaatt atataaactt ttttaactcc taatcttttt aattctttct
     9301 cagtttgtga atttagatta tttttttctg tcaataatat aggagcattt tttaatttag
     9361 caaatggtgt tacagctaat gcatctacaa tagattcact atttacaact acagcctcat
     9421 tcgcactact ccatcctttt ttgcttactt caacagcagt ttggtatctg tcctgaccta
     9481 ctaaagtatt ctccactgtt gttctactta tttcatttgc atatgctact ggtgataagc
     9541 ttaaaactag agctaagcat agcattgatg atattgattt agttaatttc atcttgactc
     9601 cccctaaaat tatagtatct aaatgtttcc tatttaaaat agtataacat aataatttaa
     9661 gtaacatcat tattataaaa tattattttt ctatctaata agcttttatt cttacttgac
     9721 attaatactt tactcatcaa atgaattttt tatataacta aagtttgtaa caacatttcc
     9781 ttagttaatc atttaaa
//
LOCUS       DAMJVX010000129         9712 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMJVX010000129 DAMJVX010000000
VERSION     DAMJVX010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.134"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            141..1109
                     /locus_tag="PC407_002753"
     CDS             141..1109
                     /locus_tag="PC407_002753"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDF2498185.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTKL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPNTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLRECIALSKTL"
     gene            complement(1226..2356)
                     /locus_tag="PC407_002754"
     CDS             complement(1226..2356)
                     /locus_tag="PC407_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2498186.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDINISDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(2509..3462)
                     /gene="msrA"
                     /locus_tag="PC407_002755"
     CDS             complement(2509..3462)
                     /gene="msrA"
                     /locus_tag="PC407_002755"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDF2498187.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRIEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEVLGYGEYLKLFE"
     gene            complement(3586..4491)
                     /locus_tag="PC407_002756"
     CDS             complement(3586..4491)
                     /locus_tag="PC407_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2498188.1"
                     /translation="MKSSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            complement(4855..6447)
                     /gene="hcp"
                     /locus_tag="PC407_002757"
                     /gene_synonym="priS"
     CDS             complement(4855..6447)
                     /gene="hcp"
                     /locus_tag="PC407_002757"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDF2498189.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKLG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            complement(6507..7232)
                     /locus_tag="PC407_002758"
     CDS             complement(6507..7232)
                     /locus_tag="PC407_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2498190.1"
                     /translation="MMRKIIKIDEGKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            7373..8101
                     /locus_tag="PC407_002759"
     CDS             7373..8101
                     /locus_tag="PC407_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2498191.1"
                     /translation="MKTNYRLHMQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEM
                     LECLGVVDKTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLF
                     GEVFACTRLHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKN
                     ILLHNKIELLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRE
                     LGKMRDEGLLSFNKNKFEIFYFQK"
     gene            complement(8265..9536)
                     /locus_tag="PC407_002760"
                     /pseudo
     CDS             complement(8265..9536)
                     /locus_tag="PC407_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
BASE COUNT         3220 a         1657 c         1137 g         3698 t
ORIGIN      
        1 cattgctttt ttaaaaattt tagtataaaa tcatattaaa taaattttct gaaataataa
       61 aatattaaaa tatccaaaaa cagcttttga taacatctat aattttcaat gtatcgttat
      121 tttaggggga gtgatttaaa atggcaataa agccaagaaa aatatctatt gtaggttcag
      181 ggcatgttgg ttctcattgt ggatttagtt taataactca aggagtttgt gatgaactct
      241 ttatgattga tatagatgaa tctaaatcaa aagctcaagc tttagattta gctgatgcag
      301 tttcatatct accacataaa gtacatatag aaaaaggaac tttttctgac tgtaaagatt
      361 ctgacatagt agtgataagt gttgcagact cttcagaagg gcctttacgt agacaaaaca
      421 caactagatt agatttacta agaccaacta ttggaatgat aaaatctata gtaaaaccca
      481 tagttgattc tggctttgat ggtatatttg tagtcatatc taatcctgtt gatgtagtta
      541 caaattatat ttgggaaaaa tctggctttc ctaaaaataa agtcattgga actggaacag
      601 ctttagattc aacaaaactt agaaggattt tatctgaaga aactggaatc gctcaacaat
      661 caattcaagc ttattctatg ggtgaacatg gagattctca aatggttcct tggtctcatg
      721 tttcaattgg tggtaaacca atcttggaca tgataaaaga caacccaaat acttatagca
      781 atttagactt accatcaata gttgaaaaaa ataaaaaaac agggataagt ataataaatg
      841 gtaaagattg tacagaattt ggtataggta ctgctttagt agaaattgta aaagctatac
      901 tacacaatga gaaaaaagtt cttccagttt ctacattact agaaggtcaa tataacgaaa
      961 gaaatgtatt tgcaggtgtt ccatgtgtta taggaaaaga tggtattgaa gaaattattg
     1021 aaatcaatat gactgaatat gagcaaaacg aatttaataa atcttgctct gttttaagag
     1081 aatgtattgc actttcaaaa acactttaga ttcaatattc tttcttacac taaaagcagg
     1141 taaatgactt attgaaaaga cttttaacta agttaatgtt taaatcattt atacctgctt
     1201 ttaattttaa tactaaaata taaacctatt ttttaccatc aaattttata actttacttt
     1261 ttttatttac ctcttgagat aacaatcctt ttttcaatcc catcaataat tctaagtctt
     1321 ctggataaag atttattcca aattcctcta aacttgatac tatactattt acactcatta
     1381 ttttattttc tatcaataat tcaactgctc tttttaaata cgttgggctt gaacctttca
     1441 ttttgtctag tggttcttct atcaaccagc cttttttatc catttcattc ataagataat
     1501 tgtatttttt gtaatttatc ttttctagat tatatgctct aaaaatcata gcagatatag
     1561 gtacaatcca ttttttcttt agttctacat aataatctaa atcttctgga tgagtcaaat
     1621 cttttaaaaa actctctttt ggcaataaaa atgcacaggc atactcatct tctgaaaatt
     1681 gcttacttgg tattcttaat tcacttgata ttatatatcc aagctcacat gccaaagtat
     1741 aatttctaat tgatgctgac tttttgtcat tacccagaga tattagatat ttagattctt
     1801 tactaattct ctgtttttga gtaaagatag ttgccccttt tctatctaca ttcatacctg
     1861 atattataat ccctttagat tctaacaaac ttaccatatt tactattgga gtactattta
     1921 aatcccaaaa ctctctagtc ttatatgcaa gagattctat atcacttata ttaatatctg
     1981 acattacttt tctatttggc aagttttctt ttggaaactt aatataattc tccataaatg
     2041 aatatatttt atgtattata attatttttt ctctataaga aatctcttct actctaggta
     2101 gtttagattg agggttaaaa tggctatcct ctacaacgat atttatattt tcatttttat
     2161 aaaaatactc ttttggaaaa tttaatatat tagataattt taatgtattt tctatatttg
     2221 gtacatactt attttcctca aaagctttta aatctttttt atttatttta gtttctttag
     2281 acaactgttc tatcgtcttt gctctaaaaa ttctagctga ttttaatctc tttccattaa
     2341 agtcttttaa attcatttgt tttctctctt tcaaaattaa attcttaatc tattggtata
     2401 aataagatta caattaataa tttatcatat tattgacaaa aaaacaaaag gtgaaaatat
     2461 ttttttatat tttcaccttt tagataagtt tataataaat attcctaatt actcaaaaag
     2521 ttttaaatat tctccatatc ctaaaacttc catattttct cttggtataa atttaagaga
     2581 agcagaattt atacaatatc ttaaacctcc aagttctttt ggaccatcat caaatacatg
     2641 acctaaatga gaatttcctt tagcacttct tacttcaatt ctgtacatac cttgtgagaa
     2701 atcacttctc tcaatgatag atttatctat aactggcttt gaaaaacttg gccacccaca
     2761 tccagaatca aatttatctt ttgaagaaaa taacacttct ccacttacaa tatcaacata
     2821 aattccctct ttgttattat cataatattc attttcaaaa ggtacttctg tcatatcatt
     2881 ttgagtgact tcaaattgta taggagttaa agtctttttt aactcttccc tatttatatt
     2941 gtttccatcc caatgacttt ttacaaaatt atatcttcca gaccctttat aatacatatt
     3001 gtagtttaat ggtctttttt tataataatc ctgatgattt tcttctgctt cgtagaattt
     3061 agttgctgga attatatcag tcactatttt actatcaaat attttactat tttccagatg
     3121 ttctcttgac tctaaagcta tatttttttg ttcttcatca tgataaaata tagcagtctt
     3181 atattgagtt cccctatcac caaattgtcc accatcatct gtaggattaa tctgtttcca
     3241 gaagatattt agaagttcat cataacttat tatatcatca tcatatgtta tttgaattgc
     3301 ttctaaatga ccagtagttc ctgaacatac ctcttgatat gtagggtttt caacatgacc
     3361 acctgtatat ccagaaacta ctttaataac tccatcatac ttatcgaaag gttttaccat
     3421 acaccaaaaa catccacctg caaaagtagc aagttttatc attttcatca ctccttaaat
     3481 aaattatact ttatttattc cctaagaagt aacttaataa tctgcatttt attattttta
     3541 cctactcaat gaaatcatgt gcaaaaatac aattgcaaat tgaatctaat ttctataaaa
     3601 gctctttttc tttatccatc caaaagcatc ttttatatca caattttctc ctaaagacat
     3661 tagatattct aatgcatcac aatcatccat agctacatct ttttccatct taggatttaa
     3721 tctatagtaa ttatctccaa gaagtttttg agaaaataac tgactcattt gagaatttcc
     3781 ttctaaggta atagatataa ttggtaaatc gggttcttta ttaattatcc agtcaatagc
     3841 tccccattca gtagtatctg cctttatact attatataca tatccagtcc ctattgaaaa
     3901 aagtcttgta ttcttaagtt taaatccact gtcaatggca tgaattatac acgctaaact
     3961 tgggtcagtt gctattattc ctccatcaac atgacgatta tatgttggaa aaaatactgg
     4021 agcagcacta cttgccattg ctacatctac aactttgcta gtctttgtaa aagaatttgg
     4081 catattgtta taaaacactg gtttccatgc attttcctca tttcctatat aaaaacttgg
     4141 tatcattact aatttattta aatcttttaa ttctagttcc tctggaaata tacttaataa
     4201 aacttctttt aagtttttgt tttcatactt tggtctgagt atttctgcat aacttttatt
     4261 aaaaatatac ttagaatttt caattgagta aagttctttt atctctttag ggcttacacc
     4321 atacgcaagt cctagtgcaa ttagactccc tgtagatgtc cctccaaaca tatttatatt
     4381 gtttaataga tttggatact cttttactat cctctcaagt atggatatac tcaatgcccc
     4441 ttttaatcca ccaccatcaa atgccaatat attaaaatta gaacttttca tatacatata
     4501 cccccacata aaacacctta tattttaatc aaaataattt cactatagta tatatatttt
     4561 atgcaagtta catattacaa tgttcctctt ttaatattta agttacattc aaacctttag
     4621 tacttaaaat aaaatatata agatattata tatctaagta ttataaattt aatacaaaaa
     4681 ttcttaaaag aatatactga aagcaggttt atttttcaat tattacaaaa aataaataaa
     4741 gggtcgtctc aaaattgatt ttttaatcaa attttaagtt taacccctta tcttaattgt
     4801 gacatactta aaaatatttt aactataaat atctttaagt atagatatat ttatttatcc
     4861 taaaatcttc ttcatatctt cttcaggagt agatataggt gtcaatgcaa atttatcaac
     4921 caatatatct aatacattct tagataagaa tgctggcaaa gaaggcccta tatagatatt
     4981 tttgatacct aaatgaagta gtgtaagaag tatacaaaca gctttctgct cataccatga
     5041 cagtaccatg ctaagaggca aatcatttac tccacattca aaagcatttg ccaatgaaac
     5101 tgccacttta attgcactat aagcatcatt acattgtccc atatccatta aacgaggtaa
     5161 gccacctata tccccaaggt ctaaatcatt aaatctatat tttccacatg caagtgtcaa
     5221 tataacagta tctttaggag cttgttttac aaattcagtg taataatttc ttccaagttt
     5281 tgcaccatca caacctccaa ccaataaaaa gtgtcttata gctccactct ttacagcatc
     5341 tactacttta tcttgtacag atgaaactgt acctcttgca aaaccagtag ttaatgtatc
     5401 tcctccatta ataccagtca tatgaacatc ttctctatat cctccaagtt caagagcttt
     5461 ttctattaca ctagaaaaat ctttatcttc atcaatatga accatacctg gataagcaac
     5521 tacttctgta gtaaatactc tatctctata actatcttta acaggcatta aacagtttgt
     5581 tgtaaataat attggagcag gaatattatc aaattctttt tgttggtttt gccatgctgt
     5641 accaaaatta ccttttagat gtttatattc ttttagtttt ggatatgcat gtgctggtaa
     5701 taactcacca tgagtatata tatttatacc cttatctttc gtttgttcta ataatttgtg
     5761 taaatcataa aggtcatgtc ctgtaactac aataaatggc cctttctcga ttttcataga
     5821 tacctgagtc ggttctggtg ttccataagt ttcagtattt gctctatcaa gcaattccat
     5881 acattttaag tttatttctc cagttttcat aactagacct aaaagttcgt caatagacaa
     5941 gtctcctcca actgctatta atccttcata gaaaaattca tttacgtctt tgtctgtata
     6001 accaagtacc attgcatgat atgcataggc agccatgcct cttaatccaa ataagattaa
     6061 tgattttaat gaacgtatat cctcattatc attccataaa tccttcatat catagttatc
     6121 atttctatta caagttgttg aacaatctga acatctaggt acaagtttac ttttttctac
     6181 ctcagttttt tcaatttgtc tttttatagc ttcatcatca aaacttacat tcgttatagt
     6241 agtgaatagc ccctctatca taattttatc tgtttcactt gttggtctat tcttatttcc
     6301 aatagttgct cttgaaagac caatcaacac tcctgtaagc tcgtcttgca agttagctgt
     6361 accccccttt ttaccacaaa caccatttat agtacaacct ttacctcctg ctgtttgttc
     6421 acattgaaaa caaaacattt tttccatttt ttattcctcc ctttttattt attaaaattt
     6481 attatatttt tctatgattc acttaattaa atttctccat ctacaatttt tccatctaca
     6541 gatatagtaa ctatttgcca tggtatcatt tttccactat tttgtagagc tgtcttaaca
     6601 gcatttacta taccaccaca gcaaggaact tccatacgtg ttactactat atttttaata
     6661 tcattttctt ttaaaatctc tgtcagtttt tcagcatagt caacttcatc aagtttaggg
     6721 catccgatta atgtaacctt atttttcata aatttattat gaaaatttcc atatgcatac
     6781 gctgtacagt ctgctgctat taaaagactt gcattcttta aatatgatgc attagttggt
     6841 actaatttta tttgtactgg ccattgatta agttgagaac cttttatctc tactatatca
     6901 ttgcttatag atgtactatt tgaaacctct ctatttattg acttagactg agaacctgga
     6961 catccacatg ctaaagtttt cttttgtgct ttctttgctt ccatatttgc ttttactgca
     7021 gcttcatcat attctgctgc ttctctatac tcaaatgaaa tcgcatttgt tggacaagtt
     7081 ggcagacaat ctcccagccc atcacaatag tcatctctta aaagttttgc ttttccattt
     7141 accattccta tagcttcttc atgacaagct tcaacacata gtccacaacc attacacttt
     7201 ccctcatcaa ttttaattat ttttctcatc atttatatca cctcataaaa tttttttaat
     7261 ttaaagtatt gatttcttat atatttattt ttaataccta tatttcttta tttcaaattt
     7321 cttgtagtag gatttttata tccccttgct tttataaact tagtatagta caatgaaaac
     7381 aaattatcgg ttgcatatgc aacaaggaag tgattatttg aaaaaatttt tagaaaagca
     7441 tatagaccta cttaaaaaaa ttcctctatt tgaaggaatt aaaagtgatg agcttgaaga
     7501 aatgttggaa tgtttaggtg ttgtagataa gacttaccca aaagatagta taatattctc
     7561 tgttggaagt gaagttactt ctattggaat tatgcttaaa ggaagtgctc atataataaa
     7621 ggaagatatc gaagggaata ggaatattgt agctgaactc ttgcctggag atttatttgg
     7681 agaggtattc gcatgtacta gactacataa aagtaatgtt acagtaacca caacatcttc
     7741 ttgcgaagtt ttatttataa agtttaaaag tgtaactggt atttgttcat ctgcatgtgt
     7801 atttcataat aggcttgttg aaaatatgct acaattaata gctgaaaaaa acattttact
     7861 tcacaataaa atagaattac tttctcgtaa aacaactcgc gagaaattgc ttatgtattt
     7921 ttcaaaacag atagaacaaa caggtagcca ccagtttaca attccctttt caagaaatga
     7981 aatggctgat tttttatgtg ttgacagaag ttctatgtca agagagcttg gcaagatgcg
     8041 agatgaaggc ttgttatctt ttaataaaaa taaatttgaa atcttttact ttcaaaaata
     8101 aactagatat ttaaacatat ttatttcaat atatactata ttagttaatt caaattataa
     8161 atatcatatt tgaatcttaa atttatattt ttacttaaaa agttaataga tatctaattt
     8221 attaaaatta agatacctat taactttatt aattaagtgt acaattagct tattttcttt
     8281 gtagtaattg ctgttttatc tataagccaa tcttttcctt ctactaatct tgaaactaac
     8341 attgatgata ctgtatttcc agttgtattt aatatagtat taggaatatc agttatagta
     8401 gttataactg ttaacaatgg aattgcctcc actggaaaac cgaagatgct aaccacgagc
     8461 atctcaccaa taagtcagcc acctgctaca ggagccataa atactccaac aacaaataca
     8521 gttataaata attgacctat cgttgctgga tttgccattc cactgcctaa aatacctgat
     8581 aaaaatgcca cttttattat tactgctaaa actgacccat ctttatgtat atttattcca
     8641 agaggtataa ctgtttctga tatatcatca ggaactccta tatttttact agcttctaaa
     8701 tttgatggta tacatgctga acttgaagaa gtagctagtg ccgttgctat tggttttata
     8761 gcatttctcc agaattgtct aaacccttgt tttcctgcag ataaaaaagc atacagactg
     8821 taaaataata agaaatacat ggctgctgtt agtgaataaa ttacaaacac ccttacatat
     8881 ccttgaatta ttttgcttcc aagttcccca attattgtag ctgtataaca gcctagccct
     8941 ataggagcat aaagcatgac tatacttact acttttaata ttgcttccgt tccttctgat
     9001 aaaaactttc ttattgtatt tcccctatct cccatcatag ctattgctgt tccaactaaa
     9061 actgcaaata taactaattg tagtaaattg ctactggaaa ataaatctcc aaaattttta
     9121 actgtaatac ttgatactac ttggtctaat aacgttcctt ttgattctaa agtttctgca
     9181 tcttgagcca ccattaaact ttttacactt gatacatcta atcctttaga cggattaaat
     9241 aataatgcag atgtaatacc taaaaatgca attacaagtg ttgttcccat aaatactata
     9301 gctgtactct tcattatttt tccaaaacgt tccatatttt ttatactagc aacacctgaa
     9361 gaaacactaa gaaaaactaa tggtactact atcattaata gagcatttat gaataaatct
     9421 ccaaatggtt ttagtattga agcttttgga ccaagtacta atcctactat cacccctatt
     9481 atagtacatg ctaaaagtat aattgatgac ttataatttt ttaaaaagtc cctcatttat
     9541 attttccttt ctaatgtttt atattataaa ttaccctaaa tcctaaaaaa attaaagtaa
     9601 attatctaat gcgcattaat aatttttgta attttcccca acagctactt tagcaaatac
     9661 tggaatatat gtcaatttct atagtttttt tgtatcttta atattttatt aa
//
LOCUS       DAMJVX010000130         9707 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMJVX010000130 DAMJVX010000000
VERSION     DAMJVX010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9707
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.135"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            71..745
                     /locus_tag="PC407_002761"
     CDS             71..745
                     /locus_tag="PC407_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2498192.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            769..1773
                     /locus_tag="PC407_002762"
     CDS             769..1773
                     /locus_tag="PC407_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2498193.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLINEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            1885..3114
                     /locus_tag="PC407_002763"
     CDS             1885..3114
                     /locus_tag="PC407_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2498194.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            3122..3796
                     /locus_tag="PC407_002764"
     CDS             3122..3796
                     /locus_tag="PC407_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498195.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLVN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            3799..5031
                     /locus_tag="PC407_002765"
     CDS             3799..5031
                     /locus_tag="PC407_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2498196.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(5317..6582)
                     /locus_tag="PC407_002766"
     CDS             complement(5317..6582)
                     /locus_tag="PC407_002766"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDF2498197.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(6635..7783)
                     /locus_tag="PC407_002767"
     CDS             complement(6635..7783)
                     /locus_tag="PC407_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2498198.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQNSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSKKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(7874..8419)
                     /locus_tag="PC407_002768"
     CDS             complement(7874..8419)
                     /locus_tag="PC407_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF2498199.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(8642..9586)
                     /locus_tag="PC407_002769"
     CDS             complement(8642..9586)
                     /locus_tag="PC407_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2498200.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLI
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
BASE COUNT         3454 a         1496 c         1303 g         3454 t
ORIGIN      
        1 tagaaatatt ttaatttatc aagtagaata tagttatgtt aatataacta ttttgaatta
       61 ggaggtaaga atgtttaaaa taatggtagt tgaagatgac gtctcattga aaaatatcat
      121 agcaaagtgt cttactaaat ggggacatga tgttcatcaa atagaaaatc ttgaaaatat
      181 aatagaagag ttcaaaaact ataacccaga attagtttta ttggacataa accttccatt
      241 ttatgatggt tttcattggt gtaatgaaat aagaaaaatt tcaaaagttc ctataatttt
      301 tatttcttca agaaattcta atatggatgt aataatgggt gtaaatttag gtgcagatga
      361 ttatatacaa aaaccttttt ctgtagatgt cttagtagct aaagttaatg cccttttaag
      421 aagaacttat aattttgttg ataataactc aaatcagatt attcacaatg gtgttacttt
      481 agatttatca actgcaacaa taaactatga agataatact attgagctaa caaaaaatga
      541 aatcaaaatt cttcatgagc ttatgaaata taaggggcag atagtaagta ggaataaact
      601 catgaaaaaa ttatgggata atgattggtt tgtagatgat aatacattaa ctgtcaatgt
      661 aaatagaata cgctcaaaat taaatgaaat tggtttagaa gattttatag aaacaaaaag
      721 aggattgggt tatataatca gttaaaacaa ttctaagtag gtgaatttat gagctttaaa
      781 gattttctca aggataaact aggtttttta gtatacaatt ttactttctt aatttttaca
      841 gtatcagtta ttatattttc tcctgtagat gtttttctga ctgatactat tttatatata
      901 ttagttgtaa atgttgtgtt tttattttta tatctattta ttagctacat caagaagaac
      961 aaatttttag ataagataaa aaatgataca ttcaaaatta atatgaatga tatagaatta
     1021 gctagatgta aaaatgaaga aaaaatatac ttgaacatta ttaaaaatca tcaatatcaa
     1081 tgtaataaca taataaataa taatatcgag aaatttgaag aaaacaaaga gattttaaat
     1141 atgtgggtac acgatgtaaa gatacctata gctataataa aactcattct tgaacaaaat
     1201 gaaaatttaa ttaatgaaaa aatttcagat gacatagata gtgaaatatt tagaattgaa
     1261 aactccgttg aaaaaatttt atatttatca agactcgaag attttcataa ggatttttta
     1321 attcaagagg taaatattga aaaaatcatt agaaatatta taaaaaaata ctcaaaatat
     1381 tttatttcaa aaaaaattat tttagactta aataatttga attttaaggt tttatctgat
     1441 gaaaaatggc tatacttcat attcgagcaa ttattgagta attccctaaa atactcctca
     1501 ttaggagata atatatctat ctattgcaaa agaaccaaaa attatttaca attaagagtg
     1561 gaagatactg gttgtggtat aaaaaaggaa gatttaaatc gaatattcaa taagggattt
     1621 acaggtaata atggaagaaa caattctaaa tctacaggtc ttgggcttta tttagtaaaa
     1681 gaactttgtg ataagcttgg tcataaaatt gatgtagatt cagagtacaa ccaatataca
     1741 aaatttacta ttacattcaa atgtgattta tagtatctta ttattgatag tttattttca
     1801 ttaacttata ttcaacttct tatagtaatt ctatatgtac atcccagtat ataattaagt
     1861 agtttttata aaaaggagga agacatgtct tttctaaaaa aatttaaaaa taaaagattt
     1921 agaaataaca gttttaaaag taacaacttt agaaatagca gttttggaag tagtggcttc
     1981 aagtctaaaa ataaaggttt taaaaacaat ccctttaaaa ataagagatt caaaatatat
     2041 gcaggaatag cagtaatcat acttttaatt ctaggagttc ttgcttatgt tgattcgaaa
     2101 aacactgaat tacaagctaa tgaaaatttt atcgaaacat atactattcc tgaaaatgaa
     2161 aaaatattta taaatgggat ggttgttcct aagcaaacta aggacttcaa tatatctgga
     2221 gattatgagt taagtgatgt taatgttaca aatggtcaaa aagtaaatca aggagacctt
     2281 ctttttactg ctaaaaatcc aactataata gcagaaatag atagtttaaa atcacaactt
     2341 agtcaataca aaaaacaaaa aatatcactt tctgatattg ctgaaaataa agatgctatt
     2401 gcttctataa atgcacagat aactgcttta aactctcaga tagcttctct ggataaaaaa
     2461 gcttatgaca gagttactgc tccttttgat ggcacagtat acttgaatga ccaaactgga
     2521 aatccagacc aaccagtttc atttatgact attcaaggtc tagagtttta tatgaaagga
     2581 caagcaagtg aacaagattt acctaaaatg aagattgacc aaatggtcaa tatactagtt
     2641 ttttcaactg accaaaagct tactggtaga ataagtttta tatctgataa accatctact
     2701 ccaaatactg aaatgggagc tcagcagaat acactatctt attatgatat aaacatagca
     2761 tttgataatc aagaaggatt agtaaatgga ttccatcttc aagcatcact tgaagtagct
     2821 aattcaagct ttaaaatacc agcatcttgt gtacttaaag acaacaaaca ctcatatgta
     2881 tttaaggatt tagatggtat attgaaaaag caaattgtag atgtagcaag tcaaaatgat
     2941 gattttgctg tagttagagg cggtcttgaa caaggcgata taataataaa acaccctact
     3001 aaagaaatga aagaaggaga cccagttcaa ggcgatggcg taactgctgg ttctaataat
     3061 ggagatacta ctccaaacaa aaaagtagaa gaagttacta tggatgtgaa ttaatttaca
     3121 tatgttaatt aaattagaaa atattcaaaa atattacaaa gtagggaaag atgaattaca
     3181 tgttttaaaa tccttaaatt tagaaattga atctggagaa tttgtcatga taatgggtaa
     3241 gtctggtagt ggtaaaacta ctttacttaa tatcttaggt tttctagatg tatttgatga
     3301 aggaagatat atatttgatg gaacagatgt aactaatttg agtgaaaatg aacgttctgt
     3361 ttttagaaat attaatattg gatttgtttt ccaacaattt aatttaatag aaactctaaa
     3421 tgtatatcaa aatgtagagt tgcctctaat ttacaataaa gcactaaaaa aatctaatag
     3481 agaagaaatt gttaaagata aattaagttc tgttgggctt ttggacaagc ttaagcagaa
     3541 accacttcaa ttatctggtg gtcaacaaca gcgtgtagcc attgcacgtt gcctagtaaa
     3601 tgaccctcag ataatctttg ctgatgaacc gactggggca ttagatagtg aaactagcag
     3661 agaaattatg gagcttttaa caaggctcaa caaacaaggt aagactataa taatggttac
     3721 acatgatcaa gatttaacta agtatgcaac taaggttata cgacttaaag atggtgtgtt
     3781 tacttcggag gtgtaatcat gagtttatta aaaaattctt tggccaattt aaaaggtcac
     3841 aagcttcgtg tatttgtagc tttactctgg attataatag gaataacttc agtaatactt
     3901 gttagttcta taggtaatgg atttcaaaaa gaaattaaga aatctgtaaa caatgtaaac
     3961 cctaataaaa ctactatttc gtttgaatca gccgataaca caggtcttac tgatgatatg
     4021 agtatttttt taaaaccctt taatgcaaaa gatttagaag aattatcatt tgttgaagga
     4081 gtagaaagaa tagctccttc aagagatggg tttaaccttg attcagttta ctcttcacaa
     4141 gcatcttttg ataaaaaaac tacgtatgtt gatgttggac cagtaaaaaa agattctaaa
     4201 ataaatttaa tttgtggtag agatttttct ttagatgatg aaaaaagaaa agtaatttta
     4261 cttacattac aaagtacaag tgaaattttt gaaaatccag aagatgcctt aggtcatggt
     4321 atcaacataa atggaactat atttgaaata ataggggtat tagatgattc tcagcaaaac
     4381 caagctggag gattctttgg aggttaccaa gatatgcaat ttactacttc cctagtacct
     4441 aaaaaggctt ttgacacttt aatgagtcaa aattcttatt caaatgaaat ttaccagtta
     4501 gacttagttt cttctaaagg ctataatgta aatgaagttg caaacaatgt tatagctaaa
     4561 ttatatgaga tgcaccctgg tatcaatggt tcttatacta caccagaccc aactgaacag
     4621 acagcttatc ttgaaagcat taattccaat gtaaacaaat atgtatctat aataactgta
     4681 gttgcaatgt ttgttggtgg tattggtgta atgaatataa tgtatgtatc tgttatggaa
     4741 agacaaagag aaattggtat aagacgtgct attggagcaa aacctaggtc tatattattt
     4801 caatttttag ttgaagctgt ttttataaca gtatgtggtg gaattttagg aactatagtt
     4861 ggatttgctg ctactaacta tgtttctaaa tatataggtt ttgaagctat acctagctta
     4921 aatagcttat tttatgcaat tgtagctact atattgactg gagttgtatt tggtctgatt
     4981 ccagccttta aagcttctaa actagaccct ataaaggcaa tttataagta gaaattgtat
     5041 aaagattttc gatttttaga ttagtttata taaaacatac ttaaatatat aacgcactat
     5101 tatatatgat agattaggaa cttaatcttt ccaaatataa tagtgcgttc tttcataaaa
     5161 aggattctct tccttactat atctaaatta ccaagatgtt atgttctaat tgttcaaact
     5221 aatacttcca attttgatta atcaatctca ttctgctgat atacaatctt ttactttcaa
     5281 tcattgtaca ctccgcctta aaaaaataat cttaaattag gcgttatata caatctcccc
     5341 attacatatt gtataattaa cctttccata taaagattta cctttaaacg gtgaattatc
     5401 agctttagaa acaaagcttt caactgtcca ttcttcttct ggattgaata taacaatgtc
     5461 tgctacagcc ccttcttcta taaatccaat atttaaattg tacaactttg caggatttat
     5521 agacattttt tctattaact gcatcatact aagatgattt ttatgcacca aatttgttat
     5581 tccaagagct aaagaagttt caagcccaat aattccactt ggagctttta taaactctct
     5641 atctttttct tctttactat gaggtgcatg gtccgttgct attatttcaa tagtaccatc
     5701 atttaatccc tctataatct tatctctgtc ccatttactt cttagaggtg gattcatttt
     5761 tgcatttgtt ccaaactcca atacatcttc ctcagtcaat gtaaagtgat gtggtgaagc
     5821 ttctgcaact acatttgcac ctaagtattt tgcaaaccta accatatcaa cagatacacc
     5881 tgaacttata tgttgtatat caactttagc accagtcttt agtgccaaca tacaatctct
     5941 ggctaccata acatcttctg ctacacttga tgcacctttt aatcctagtt ctgaagctac
     6001 ctttcctgca tttacacctg gatttccaac taaagaaggg tcttcttcat gaaaactaag
     6061 tggtacatcc aactcttttg ccttaatcat tgcctccata attagattgc tatccattaa
     6121 aggtaatcca tcatcagtaa accctggtac accagctttt ttaagagctt ccatatcaac
     6181 taactcttta ccttcaaaac ctttagttat agctgctgct tgtaaaacat ttatacatgc
     6241 attttttgac ttttctttaa cataattaaa agtatcttca ttatctacaa taggattagt
     6301 atttgccata catataacag ttgtaaatcc acctctagca gcagatttag ctccactttc
     6361 tatatcttct ttatatgtaa acccagggtc tctaaagtgt acatgtacat ccactaaacc
     6421 tggagcaact acattaccac tagcatctat tattttttca tattcatcac tttcatcaaa
     6481 cttgcctatt ttataaattt tattttcttt tataattaag tcaatattct catctctttg
     6541 acttttaggg tctattactc taccattttt aattaaaatc atattatttc tcctttatat
     6601 ttttaattta tatagactca tataatatta tacattattt tacatactta aatacaaaat
     6661 tacgtttgac atcaatagcc tttttaggac aaagttcatg gcaacagtaa catcttatac
     6721 atttacttaa gtctataata gcacctttag tccctatctc tatagtggat gcaggacaag
     6781 cttccttgca ttttccacat tttatgcaat cttgaaatct taccacaggc tttggtgtta
     6841 taagctttga aataggttca tttaaaaatt ttggtagctt tacggaaaac aatcttaaat
     6901 ccttacttac atttggaatc ttgtaatttt ttataattaa atcttttata tcttcaccta
     6961 aaatttcaat atctgaaaaa tcttcttgta tatagcctct cttaatagca cctctaattg
     7021 tagcaactct atttggaagt aaacttataa tcttacatgc aacaacatct atagcatatg
     7081 gattttgtga tgctaataaa actcctattt ctcgagtagt accagctgaa ggtccatcac
     7141 cttccatacc tactatacca tccataattg tcaatgttgg ttttatataa tcacaaatat
     7201 ctaaaagtac attttcgcaa aacaactctt ctgtaggaaa tctataatgt atctctgctt
     7261 ttttcaatcc tgctatgcat ccatataagt tttttgtacc accagtaaag gttgccatta
     7321 tatgagtctt taatttacaa agatttatta tataatctga atctactatt ggtttaataa
     7381 tatccatata ttttagaaca tgtgcttttt tactatgtat cttaacatct gaaacatcat
     7441 aatttaactt aatgcccagt tcatttgcta cttcttcaat tccagatgcc ttataaatgc
     7501 tcttaagaga attttgatta tatggaccac cagggctatc tgctattgta atatcacaat
     7561 ttaactctaa aagtttttca catacaactt ttacaaccat aggatgtgta gttgtagctt
     7621 cttcaggctt ttttttcatt aataaatttg gttttataag tactttgctt ccttttttaa
     7681 caaacttatc tataccacca atatcctcta aatttttttc aatagataac tttacttctt
     7741 cataattgta agttttacaa ctcctaacag ataccgactt catattatct ccatccttct
     7801 atcacaacag tttatttgta taatttaaaa aaggagaaac caaagtttct ccttttaatc
     7861 tatgttttaa ttattatttt ccaaaatgtc tgtttaataa tcctaagaat gctttaccat
     7921 gtctagcttc gtctttacac atttcatgta ctgtatcatg tatagcatct aatcctaatt
     7981 ctttagctct cttagctaat tttaattttc catcagttgc accatactca gcatcaactc
     8041 taactcttaa gttttctttt gtatctgcaa ctacaacttc tccaagaagt tctgcaaatt
     8101 tagcagcatg ttcagcctct tcaaaagcta ttctcttata tgcttcagct acttctggat
     8161 aaccttctct atcagcttgt ctactcattg ctaaatacat tcctacttct gtacactcac
     8221 cagtaaagtt agctcttaat ccttcgatta tttctgcatc tacaccttga gctactccta
     8281 ttctatgttc atcagcccaa accatttcgc ctttcatttc ttcaaattta tcagctccaa
     8341 ctttacatac tggacattgt gctggtgcag catctccttc atgtatatat ccacatactg
     8401 tacaaacaaa ttttttcata attaatttcc tcccaatttt atataattaa atttttattt
     8461 ttaagtttaa taacttgcta caagaatata tataccccag actttatagc ttaaacacta
     8521 aaaataaaat ttatttccta attaatttta tcactttgaa acaccagtat caaccaagtt
     8581 ttgatgtcaa atttctacat aatacataac ttatttgatt aattaggaaa tagttgtcat
     8641 attaaatact ttctaatgaa gactcctctg aatctttaag tttcactttt ttgctcttta
     8701 aaaaagataa tttagtttct gcagttatga ttgcaacaaa tatcaagata ctaccaataa
     8761 tcaattttgc tgtaataagt tcctttagaa ttattataga aaatattgta ccaaatacag
     8821 cctctgtgga taatattata gctgttctag ttccatcaac tcttttttga catatagtct
     8881 gaaataaaaa tccaattgta gtactaaata cagctagata catagttccc atataaccag
     8941 ataattcagc ttccatcttt aattgacctg caaatgtttg aactacaaca gacattaaaa
     9001 acgctactgt aaattgaata gctgtcaatg ccattgggtt gttatctttt gcaaactcac
     9061 ttgtaaagaa tatatgaaat gcaaatccga atgaacatat aagtgttaga aaatctccaa
     9121 aattaataga gaaatcagct tctaaagaaa gtatcccaat tcctataaga gctaccaaac
     9181 tacttattat tcctatttta tcaagttttc ttctataaag tataaatcct atgaacggaa
     9241 ctattacaac attagctgct gttataaatg catttttaga tggtgtagtg tacataagtc
     9301 caatagtttg tactgtgaat gctagaaata aaaatattcc taaaatccct cctgctttta
     9361 ataatttttt acccatattg gcacgaatct gtttaaaaaa cagtaaattc ataattatag
     9421 ctgccaagaa aaatcttaaa gttatgattt gtagaggtgt aagcccaccg tctaatgcaa
     9481 attgggttgc tacaaagcca cttccccata taattgctat aaatattagt cctatttctc
     9541 caaagtattt tttcaataaa gccccctcct ttttattttt ttgcaaaatt ataaaccccc
     9601 taaacagcaa gagagttttt ctataagcat tactataaat taatatagta aattttttca
     9661 gtattttaaa tctaaattta catacatttt gagctcaaat ttatatc
//
LOCUS       DAMJVX010000131         9679 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMJVX010000131 DAMJVX010000000
VERSION     DAMJVX010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9679
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.136"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            963..2321
                     /locus_tag="PC407_002770"
     CDS             963..2321
                     /locus_tag="PC407_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2498201.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     ASGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2427..3221
                     /locus_tag="PC407_002771"
     CDS             2427..3221
                     /locus_tag="PC407_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2498202.1"
                     /translation="MVTIKEYSIPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3214..5520
                     /locus_tag="PC407_002772"
     CDS             3214..5520
                     /locus_tag="PC407_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2498203.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVAESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRIIRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5659..6936
                     /locus_tag="PC407_002773"
     CDS             5659..6936
                     /locus_tag="PC407_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2498204.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIESLKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7119..7841
                     /gene="yqeC"
                     /locus_tag="PC407_002774"
     CDS             7119..7841
                     /gene="yqeC"
                     /locus_tag="PC407_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDF2498205.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQKFCVRY"
     gene            7948..8517
                     /gene="mocA"
                     /locus_tag="PC407_002775"
     CDS             7948..8517
                     /gene="mocA"
                     /locus_tag="PC407_002775"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDF2498206.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            8514..9533
                     /locus_tag="PC407_002776"
     CDS             8514..9533
                     /locus_tag="PC407_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HDF2498207.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT         3821 a         1009 c         1816 g         3033 t
ORIGIN      
        1 tagatagaaa cttgtatatt ttatgttgca gatacaaata tagtaaagga gagtacattg
       61 gtacaactga gatacatagc ttattaagtt tttctgtctt atgagaatta tattataaaa
      121 tattcataaa tattttgagt ttaaattaaa taattatttt atgcttagat taattgatat
      181 ttaattaatt taataacaaa aacagtgaaa gagtaaataa tattatttac agggggtaaa
      241 gcaaagtatg ttaaataaat tgtacttttg gccccgtaca tagagaaatc tatgtaggac
      301 acaactttaa aaccagtaaa tcctaaagcc ttgcaactac aataaaagat agaaagaaac
      361 ttttatgaat gtggtgaaaa ccagaaacaa tagtgaggat ggtatatggt gagagcctaa
      421 gtacttaaaa atggacattt ggtcgccaag cctagaaata ggaaggtcaa acgactatct
      481 cgagagagag tacactataa gcgattgata gtggaaacgg gttgctctta gcaggtaatg
      541 ctgaagatga agaaatagtc tacgctcata tgaaagtatg aggtcactag atttgaagtg
      601 acatctataa agtagcgttt ataggtaaat gtttaaaact tgaaacatgt aaatacttat
      661 gaaaacttat aacttataat gattaagatt gaaagattta aattgaatta aatttactat
      721 aaaaaattaa aaattcatag ctataatact cttatatttg taaagcatgg tatataatgt
      781 atacaaaatt aaagaatacg tgtatttgtt aataattaaa tttattgcat ctgtttttta
      841 agactaaaaa cttaaatata tgttggagta tagttgatat ttatgaacat cattaattta
      901 atatattaga tttttaagta tttatataat tcaataagtt ttatgtaacg ggagttttta
      961 ttatgaaagc aaaaagtaca tcaaaatttg aattagacgg aataccacca ctaaaggaag
     1021 caataccttt agggttacaa cacttaatgg caatggtaat aggtagttgt ttaccagcga
     1081 tattgattgc aaatgcatct ggacttaatc ataagatgtc aacattattg attcaagccg
     1141 cattagtttt tgcaggatta tcaactttat tacaattata tccaatacct ttgtttaaag
     1201 gttttaaatt aggaggagga ataccagtta taatgggagc tacagctatg tttataggag
     1261 ctggggtatc tgttgcaagt aagtatggac taccagtatt atttggctct caaataatag
     1321 ctggtatagt aataattata attggttttg gtcttaaaaa aatacgattt atatttactc
     1381 cagctgtatc aggaacaata gttgcatgta tgggtcttgg actttttcca atagctataa
     1441 aaaacttagc tgggggtatg ggcaatgaga cttttgggaa tccaattaat tttatagttg
     1501 gaatatctgt atctcttatg atattaggat taaataagta tggaaaagga ttatttaaag
     1561 atgcatcaat attagttagc ataatttttg gatacatttt atcattaatt ttgggtatag
     1621 taaatttttc ttcaattcag gagtttactt tggttgcgct tccaaaacca cttgcttttg
     1681 ggctagatat aagacttgaa gttgttgtaa tgttttcaat aatatattta gttgagatag
     1741 ctgatataat gggtgcttgt acactttctg cggtaggagg gcttaataga caagtaacag
     1801 atgaagaact ttcttctgct gttttaggaa atggagtact ctctacaata ggtgcattat
     1861 tttctgcaac tccaatgggg atgtttggac aaaattctgc aatcgtttcc aatacaaagg
     1921 ttgtaagtaa atttgtacta gctattggag gaattggatt atttttggca ggaatatcgc
     1981 ctttattagc taatttaata agaacaattc ctccctgtgt ggttggagga gcaacactcg
     2041 ttatattttc aactcttaca acatctggtt taagacttgt tagtatggat ggatttaatc
     2101 aagaaaatag catgatatta ggtctttcta tggcttctgg tattggattc atggtagctc
     2161 ctcaagttct tgaaaaattt ccaaaattta tagagacatt acttgcagat tcaagtgtag
     2221 tttcaggagc aatggtagcc ataataattc aagctttata tatggtaaaa tttcctggaa
     2281 ataaaagtga taaggttgag gataaaaata aaagtttata atgtaaaggt ttaatatatg
     2341 tgaattaaat atactacaat tataaatata aataaaagat ttattaaaaa cttttgtgtt
     2401 ttaataagtt aatgatgggg gataagatgg ttactataaa ggaatattca attccacaga
     2461 gtttagaaga agcatataaa attttaattt ctaaaaaaaa taatgttatt ttaggtggat
     2521 gtggatttat aaaattaagt aataaaaata taggaacagc gatagattta aaagatataa
     2581 atctaaatta tataaaagag gatgaaaaaa atattttgat aggtgcagat acttcactta
     2641 gaagtttaga aattgataaa actataaaaa attactgtag tggaatactt tcaagcgctg
     2701 tttctaatat agttggtgtt caatttagac aaggggctag aattggtgca agtgtatttt
     2761 caaagtatgg cttttctgac ttaataccag cgcttttggt tgttggagcg aaggtgagat
     2821 tatacaataa aggaatacta gagttaagtg agtttttgga tagtgaatta agtagggata
     2881 tattggtcga agtaatacta ccaaaaaaag atgcgatagc tgtttttgac tcaataagaa
     2941 aatgcacagg agatttttct gtattaaatt cagcaatgct aaaagaaaat gatacttaca
     3001 aaatagcaat tggggcaaga cctcaaaaag ctagattagc acttgaagcg agtaatatat
     3061 taaataaaga aaaagatata gataaggcat ctattgttgc tagtaaggaa cttacttatg
     3121 gaagtaatat gagagctagt aaagagtaca gaaaggatat ggcaagtgca ttagttaaaa
     3181 ggatgtataa tgctattgag gagggaatgt ataatgatta ataaggggat aagaaagata
     3241 gatagtacag caattgtcac aggaaaacct ctatatactg aagacctcat tatgcataaa
     3301 gatgttttga cattaaaact acttagaagt ccacatgctc atgctaagat aaagaatata
     3361 gatacatcaa aagccgaaaa aattccaggg gttgtgggta tttatacgta taaagatgta
     3421 ccacaggtca gatattgtgt gccaggtcaa acagaaccag aagcatcacc ttatgataga
     3481 gttttacttg aagatacagt tagatttatt ggagatgaag tagctttagt ggctgctgaa
     3541 gatgagaaaa cagcacttaa agccttaaaa atgataaagg tagattatga ggttttaaag
     3601 cctgtgtttg atgcaagaga agcagaagga aatgaagttg ttatacatcc agaaaaagac
     3661 atagtagagc catacccttt tggattggat gcaaaaaata atatagtatc aaaagaattt
     3721 tttgaatttg gagatgttga agctaagctt aaagaaagtg attttgtggc tgaatcttca
     3781 tacaaaaccc aaagtcaatc gcattgcatg atggaaactc ataggtcata ttcatataaa
     3841 gatataaatg atagaattgt tgtagtttca tcattacaat caccacacaa tgtaaagaga
     3901 ataattagaa aagtattggg tataccatct tcaaaaataa gagtaattaa acctagaata
     3961 ggtggtggat ttggaggaaa gaatatagca gcaacagata tatttactgc atttgttact
     4021 atgaaaactg gtagaccagc aaaatttgta tttacaagaa gtgaaacttt tgtaggttct
     4081 aatagtagac atgaaatgtt ttttgatgtt aaagttggtg cagataaaga aggaaattta
     4141 aaagcaatag agctaaagtc attaaacaat acaggtgctt atggtgggaa tggtgtctct
     4201 gtttcttccg aatctggaca caatacctta ccaatatata atgatattga tgctgttaga
     4261 tttaatgcta gaacagtata tactaatagg ttaccagcag gagcacttag agggtatggt
     4321 gctacacagg gaatatttgc aatagatagt gctattgatg aattggcaca taagattgga
     4381 atggacccac ttgaatttag aattaaaaat ataatagata ggtataccaa tggaaaagca
     4441 gctgaagacg aaatattaag ttgtgagctg agacaatgta tagaaaaggg caagaaatta
     4501 atcaattggg atgataaata tccatgtaaa gatataggaa acaacaaagt aagagcagta
     4561 ggtatggcag tagcaaatca tggttctggt ataccgcgtg tagatatggc aacagtcact
     4621 ataagaatgg atgaagatgg cacatataaa ctattatctg gtcttgctga cttaggtcaa
     4681 ggggctgata caattcaagc tcaaattgct gcggaagctt taaatactac tgtagataga
     4741 atttctattt atacaggtga ttcagattta tgtccctatg atacaggagc atttgcttct
     4801 tgtacaacaa cggttacagg aaatgcaaca ataaagacag caaataaatt aaaagatata
     4861 ttactttcag ttgcagagtc aaagctagga tttaaaaaag aagatttaga gttgggaaaa
     4921 gacagggttt ttgttaaaaa cgataatagc aagtttgttc tattagagga attgggtaga
     4981 gaatctgttg gaggaatagg atttgctagt gatgatgtac cagtagtttt ggaagcaaca
     5041 gaatcttttg gcatgttgaa aaaaataaaa ccatttatag caggatttgc agagatagaa
     5101 ttagataaaa atacaggcga atataaggtc ataaattttg catgtgtacc tgattgtgga
     5161 acagttataa atccaacttt agctagaata caagtagaag gtggtgtgat gatgggtata
     5221 gggtttgcaa tgtatgaaga ccctatattt gataaagatg ggagattatt aacagatagt
     5281 ttcctacagt ataaagttcc tacaaaaaaa gaagttggaa atatattggt agattttgct
     5341 gagaatttcg acccaggagg tccttatggt gctaaatcat taggagaaat agttatacat
     5401 acaccagcgc cagctatagc aaatgcaata tttaatgcta caggagttcg tttaagagag
     5461 cttcctatga cttttgaaaa agtttatatg ggtatgaaaa ataataataa acaattataa
     5521 taaataataa ggtgtataaa agcaagtaaa tttaaattat tttgtaaaat aaagtaatta
     5581 aatggacatt atatatggtg ttttgtaaaa ataccgtata taatgtctag tagagtatat
     5641 tctaaaggag gtgagttcat gtcaaacaga ataaaaaata tacactatgg aactcagaga
     5701 caacaattaa aaaacttaac taaaaaagaa tatcaagctc tacgagagat gtgcttctta
     5761 gctaagaact tatataatgt tggtacatat aatgtaagac agcattattt caatcaagga
     5821 gaacatctta actatgaagc taattatcat gtatgcaaag aaaatgagaa ctataagctt
     5881 ctcaatagta atatagcgca acaaatatta aaagaagtag acagttcgtt taggtcattt
     5941 tttgggttaa ttaaattagc caaagaaggg aaatatgact ttagagggat aaaatcacct
     6001 aaatatttag ataaagaagg attttttagc ataataatag gtcaaataag aatcaaagaa
     6061 gatgggattt taaatgtacc tatgtctcca gcatttaaaa aactatatgg gaaagtaagt
     6121 ataaaagttc cacaaaatat actagagaaa aaaattaaag aaataagaat aatacctaaa
     6181 cataaagcta ggttctttga aattcagtat tgttatgaaa taccgaaatc agaggaagtt
     6241 tcaaatcaaa aaaatgcact agctatagac ttgggtttag aaaatttatg tacatgtgtt
     6301 accaatttag gtgatagttt tatcatagat ggaaaaaggc taaaatctgt gaatcagtgg
     6361 gcaaacaaac agaatgataa aatagaatcc ttaaaattaa aaaagaatat aactgcaata
     6421 acaagaaaac aatttaaagt ttggaataag agaaataatc aagttaaaga ctatgtaaat
     6481 aaaacctgtt tttatataat tgattattgt gtaaaaaata atattggaaa tttaattgta
     6541 ggatataatg ctaagttaga aaaaagtgat aatatggaga gaaaaatata tagaaatttt
     6601 ataagaatcc catttggaga gataaaagga aaattagaat atctatgtaa aataaaaaat
     6661 atcaatttta ttaggcaaga agaaagctat actagtaagt ctgatttttt tgctgatgat
     6721 tttctacctg atatcaatgt ggacaatgtt aaaaaatatg actttaaagg ggaaagaata
     6781 acacgtggac agtacaaatc cagtgttgga atcattataa atgcagatgt aaatgcgagt
     6841 cttaatatac taaaaaaatc acaagtagtt aatttatcta atttacagaa taatcctcaa
     6901 atattaaagc aaccacagag agtaagaata tcttaattat atttaaaaga taaactgata
     6961 gcgtaagctt acagcttagc ggcgaattgg atccgccttg ttgtttacaa cagaatctat
     7021 ttactttcga gtttatggaa tttcaaaaat cacttttact aaaaaggtct aactataata
     7081 attaagagat tagaaaactg gttataagag gtgaaagtgt ggaactatca aatataatag
     7141 aaaagaatga aataataact gtagttggag caggaggaaa aacttcgttt ataaactatt
     7201 ttgcaaattt ttatagagat aaattgaaag ttcttcttac aacaacaact aaaatatatg
     7261 taccaaacga ttatgacaat ataattatta caatagatgg aactgttatt ccatctattt
     7321 gtcatggaat aacagtctgt ggcagttata taaataatga aaataaatta gtaagtattg
     7381 attcctctat tttggatgaa attgtagacc aatttgattt agttttaata gagggagatg
     7441 gctcaaagag aaaaaaactt aaaggttgga atgctaaaga accagtagta tatcataaaa
     7501 ctacaaaaac catagggata ttagatataa catcatttgg aatgaatatt aatgaagaga
     7561 atatacatag agtagaaata tttaaaaaaa tagcaaattt agatacaagt agtattaata
     7621 gtagttctac agtaagtatt gaaaacttaa aaaatatagt attaaatcca aatggattat
     7681 ttaaaaatta ttctggaaaa agagtattat ttattaataa agttgaaaat gaaaaatata
     7741 aaaatttagc tataaaatta atagaaaata ttaaagaata tgaaagtgaa atagagatat
     7801 tttatggaag cgtaaaacaa aaattttgtg taagatattg aagtattaaa atagtttata
     7861 tgatattata tatatagata atatcaatag agatattata aaaatcatag aactatcaat
     7921 atattataaa taccaaggat gatatatatg ataaatgcag tgataatggc atcaggcttt
     7981 gccagaagaa tgggagcaaa taaactttta ttaaattata aaggctgttc tataatagaa
     8041 catgtattta gagaagttag caagattgat tttcatcaag ttgtagtagt aagtcaatat
     8101 gaagaggttt tgttattgtg taataaatat ggttttgaat atatagataa taaaaatgca
     8161 aatataggtc aaagtgaatc tataaaatta ggagttttaa atagctcaaa atgtgatggt
     8221 tatatgttct ttgtaggaga ccagccattt attgataact tatatataga taaaattata
     8281 aatactttta aattagatag agattttata gtaattccaa gatacaaaga tatgtgtgga
     8341 aatccagtca tattcccata tagtaagaaa gaagaattgt tggcacttaa agaagatgaa
     8401 aaaggtaaaa ccattttaaa aaattcatca aaaataaagt atgtagaagt tcctaaagaa
     8461 atgttatttg atatcgatac aaaaatggac tatgagagaa tcgggggtaa attatgaagc
     8521 taataaaaac tatagatgct gttggacaag ttttatgtca cgacataact caaataatac
     8581 ctggagaatt taaaggaaga aaatttaaaa agggtcacat agtaaaagaa gaagatattc
     8641 cagtgttatt atctcttggt aaagataatt tgtatgtatg ggaaaagtca gaaggaatgg
     8701 ttcatgaaaa tgaaggcgca ttgtttttaa aagaactaac tgctggagaa aatttagatt
     8761 ttagtgaaat aaaagaagga aaaatagatt ttatagcagc atgtgatggt ctattaaaga
     8821 tagatgttga tgcacttttt gatttgaatt gtgttggaga aataatgatg gctactcttc
     8881 ataataattt tatagtaaat aaaggtttaa aagtagctgg aacaagagtg atacctttgg
     8941 ttatagatga gaaaaaatta gaagatgcaa aaaaagtagt tggtaataga aaaatagtaa
     9001 atgtagtacc ttttaaacct aagaaagtag gaattgttac tacaggaaat gaagtgtttt
     9061 atagcagaat tgttgataaa tttggaccag taatagaaga aaaagtaaaa ggctttggtt
     9121 gtgaagttat aggacagact atatgcccag atgataaaga aattataaag agtgcaataa
     9181 aagaatttat aagtcaagga gcagaactta tctgttgtac tggcgggatg agtgtagacc
     9241 cagatgatgt tacgccaaca gctataaaag aaactggagc tgatttagtg acatatggtt
     9301 caccaatatt accaggagct atgtttttac ttgcttatta tggagaagtt ccaattatgg
     9361 gtataccagg atgcgcaatg tatcataaaa ctacagtatt tgatatagtt ttatctaggg
     9421 ttttaattga tgaaaagctt gataagtatg atatagctag atatggtcat ggtggccttt
     9481 gtatgaactg tgatgtatgt acttatcctg catgtaattt tgctaaaata taaagttatt
     9541 aaaaaagagt atttacaaat taagtactct ttttaaatat gaaacaaaac agctagaatg
     9601 atgttttaag aaaagcatat ttatttagaa attataataa cttattttca atgtgtaaaa
     9661 gaatgaataa agttgcaat
//
LOCUS       DAMJVX010000132         9435 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMJVX010000132 DAMJVX010000000
VERSION     DAMJVX010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9435
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.137"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            496..1701
                     /locus_tag="PC407_002777"
     CDS             496..1701
                     /locus_tag="PC407_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2498208.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVGPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNVKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1772..1993)
                     /locus_tag="PC407_002778"
     CDS             complement(1772..1993)
                     /locus_tag="PC407_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498209.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2134..2733)
                     /gene="recR"
                     /locus_tag="PC407_002779"
     CDS             complement(2134..2733)
                     /gene="recR"
                     /locus_tag="PC407_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDF2498210.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2746..3096)
                     /locus_tag="PC407_002780"
     CDS             complement(2746..3096)
                     /locus_tag="PC407_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDF2498211.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3155..4792)
                     /gene="dnaX"
                     /locus_tag="PC407_002781"
     CDS             complement(3155..4792)
                     /gene="dnaX"
                     /locus_tag="PC407_002781"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDF2498212.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKYSIDENESK"
     gene            complement(4869..5133)
                     /gene="ffs"
                     /locus_tag="PC407_002782"
     ncRNA           complement(4869..5133)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PC407_002782"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5171..5262)
                     /locus_tag="PC407_002783"
     tRNA            complement(5171..5262)
                     /locus_tag="PC407_002783"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5226..5228),aa:Ser,seq:gga)
     gene            complement(5263..5718)
                     /locus_tag="PC407_002784"
     CDS             complement(5263..5718)
                     /locus_tag="PC407_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDF2498213.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(6038..7309)
                     /gene="serS"
                     /locus_tag="PC407_002785"
     CDS             complement(6038..7309)
                     /gene="serS"
                     /locus_tag="PC407_002785"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2498214.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKTRLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEYKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7434..7640)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7767..8648)
                     /locus_tag="PC407_002786"
     CDS             complement(7767..8648)
                     /locus_tag="PC407_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2498215.1"
                     /translation="MNFLSLAKAASTNSNITKRTLENMLNNFSDNIPNIIYAIIVFVI
                     GIYISKIVRKMVSRFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGVIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFINEKR
                     ILDDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENNIKVPFVP
                     SSLLFNNMSNSNTMDKR"
     gene            complement(8660..9169)
                     /locus_tag="PC407_002787"
     CDS             complement(8660..9169)
                     /locus_tag="PC407_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDF2498216.1"
                     /translation="MNSDERRSKLIDILKESKCPVKGGMLAEILNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHRNSKEIYDELKIIVDLGGIVKDVIVNH
                     PTYGQITAELNIHSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIETLKEETIEVITK
                     ELEDRGILS"
     gene            complement(9296..9412)
                     /gene="rrf"
                     /locus_tag="PC407_002788"
     rRNA            complement(9296..9412)
                     /gene="rrf"
                     /locus_tag="PC407_002788"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3000 a         1657 c         1017 g         3761 t
ORIGIN      
        1 ctcagatact attgttatca aaatatctgg ctttatggtt tgtttcttaa tataaattaa
       61 cctactgttt aattttcaat gttcgtgttt atttcttttt ccttttttca aggacaagta
      121 atactatatc atctttatct attctagtca atataatttt ttactttttt taaatttttt
      181 atttatcatg tattctttat atttaatttt taaaacttaa tgctaagtaa acctatttat
      241 ttcataaaac taatatatat tctatgctct attttccttt ttattctata tttaaatttt
      301 ttatttacaa gaatattttt aatataacat atttgataaa attaatttac atttctttca
      361 cttgtaattt ttgcaatctt gtattagatt aatattaaaa ataatttatc aataacctta
      421 ttcacaaaga cagtttttat atatcaaaat ataatacaat ataaatacta tgctacattt
      481 taggaggatt ttattatgag caaaaaagta ttaataatgt ctgcttccac tggtggtggg
      541 cataataggg ctgctctagc tattaaagaa gaacttactt ccaaaacact tgatggagag
      601 ccaattgaat gtgaaataat agatagtttg aaattagtta ataatacaat ggataaaata
      661 atttcaagag gatacgaaaa atctgctata tatactccca aagcctatgg aagtgtatat
      721 agattatctg aaacaaatct tttgtcaaaa aatgaattta aagataatct actaataaca
      781 ttcatggcta aaaaatttaa aaagcttatt agaagcgaaa aaccagactt gattatagga
      841 acacatcctt ttccaatgat tgctctaagc acattgaaaa aaaactttaa tttacataat
      901 aatgaatcaa atgcttacac agagcatttt tataaacatt atacaaatac cattaatgtt
      961 cctccgctta tttctgtatt aaccgattat actactcatt ctacatggat acaaaatgaa
     1021 attgattact atattgttgg tcatgaatat gtaaaagaat tattggtgtt tgatggagtt
     1081 ggaccgagta aaattagaac atttggaatc cctgttgaaa aatcttttct ctctcacaga
     1141 gataaagata ttgttctttc tgagttgaat ttatctcctg ataaattaac tgttttactt
     1201 atggggggta gctttggggc tggaaatata aaagagacac ttgatgaatt actggatact
     1261 gatagagatt ttcaaatact tgtaattact ggtaagaatg aaagcctaaa agaaaaaatc
     1321 gaaaaaaaac ttatgtctcg ttatcatgat aaaaatgtat gtgttcttgg atacactgat
     1381 aagatgaatg atattttagc ttctatagat gtattagtaa gcaaacctgg tggacttact
     1441 acaactgagg ctcttttaaa agatgtacct atgatagttc cttattatat accaggtcag
     1501 gaagaagaga atcttgattt cttatctaat tgtggtgcag cacttagaac tacaaaaaaa
     1561 tataacttgt caatattact aaaagttcta atagatgacc ctagcagatt agaaatgcta
     1621 aaaagaaatg ttaaatcaat aagaaagtca aattcagccc aaaatatagc taatttaatt
     1681 ttaaatataa tttctggttg ataaaataaa taaaatccct atagtatata tattatctat
     1741 tagggatttt ttattttcta attatttaat attaaattgg ttcataaaag tcttcttcat
     1801 aatcatactg ataattttta taattatttc taaattcagc atttttattt cgtacatttt
     1861 ttttagtttt tttcttatta gaaaaactta tatcctcaaa atcatcatat ttaggtttat
     1921 cttttgattt cattttttta actctttttt ctttcattgc attatgttct tctaagttca
     1981 taattggttt catgttgcac ccctatcaaa atttttaaat gatttataca acaaattata
     2041 caatacagaa gaaatttatt caataaatct aaatcaaaat taatttgaat ttaactcaaa
     2101 aaactacaaa aatcgaggta ttaacctcga tttttatatt tctcttctgc cttctagtgc
     2161 ttttgatatt gtgacctcat ctgcatactc taaatcgcct ccaacaggaa gaccatgagc
     2221 tatccgagtg actttgattc ccattggttt tactaaccta gcaatataca tggctgtcgc
     2281 ttcaccttca atagtaggat ttgttgccat tattatttct ttgacttcct gattgccaag
     2341 tctttggata agttccttta tttttaacat atctggtcca attccatcca ttggagaaat
     2401 tactccattt aatacatgat actgtccttt aaactctctc gttctctcca tagctgctac
     2461 atctcttgga tcttccacaa cacatataac acttgaatct ctacttttat ttgaacacat
     2521 gctacatggg tctgtatctg ttatattaca acaaattgag caatatctta tttctctttt
     2581 tgcatctata atcgctttag ataaagcttc cacatcatta gtattcatat taataatatg
     2641 aaaagccaat ctttgagcag tctttcttcc tactccagga agctttgaaa attcctctat
     2701 aagtctcgtt atgggacctg tataaacttg cataaatacc acctactaga ataacccagg
     2761 tatattcata cctccagtta ccttactcat ttgacttgct tgtatatcat caatatttct
     2821 caatgcttga tttactgcac ttaaaactaa atcttgtagc atttctatat catctggatc
     2881 aactacttct ggcttgatag ttatatctat aacttctttt ttaccattaa cttttacagt
     2941 aactgctcct ccaccaactg aagcttctac ttctttactt tctaactctt gttgagcttt
     3001 ttgcatattt tcttgcattt tttgagcctg ctttaacaag ttgttcatat ttccaccacc
     3061 aggcatcatt ccgcctccaa aacctttttt agccataaac actattcctc ctaaacttat
     3121 tttcatttta ttattatatc ataatagtat tttattattt actctcattt tcatcaatac
     3181 tatattttat ttctaatatc tcttcactca ctatgttctt taatatctcc tcaccaatgt
     3241 cctttttctc tttaacttca agatttatat ctttaacttc tgatgtcagt actattttta
     3301 cattaaaact tctatttaaa acatctctta ttgttttttc taaataattt attgtatctt
     3361 gagagctcag tctagttttt gcaaaatcaa atttatcatc aaatataaca tacaacatat
     3421 tagaatatac attaaaactt tttacttcac ttaagagtgc gtatattggc atttttttat
     3481 cttttttaat ttgtttaagt atattcttcc aagatgactc tataagtata acgtcctcat
     3541 tttttacatc ttcataaact acattttctt gttttacatc tatttctcta tcgacctcat
     3601 actctttttc tttattattc ccaatttttt cagacttaaa gttccccagt tcaatcattt
     3661 tttctaagtt ctcaactctt tttataagag cttctttact ttcatcaaac attggttgag
     3721 caattttcat tatggttatt tccactaaaa cccttggatt tgaagatgac tttatatcat
     3781 cctgagttat tgataatata tttaaaatcc ttattaaatc atttatatct atatttttag
     3841 actgaatttt aagttgttct atagtttctt ctggtaatga aattatttca tccaattctg
     3901 atgaaacctt acataccatt aaatttctaa aatgatcaat taagtcattt ataagatttc
     3961 ttatatcttt gccccataag acaaattcat ttaatatctc aagagacttt ttagtgtcct
     4021 gctctacaat acattgagac aattcaaata attgttctat atttacgctt cccattaatt
     4081 caacaacatc tttataatct atcttacttt ctccaaaaga catacattgg tctaatatac
     4141 taagtgcatc tctaagagct ccttgagaat ttctagctat taaattaaga gctttatcat
     4201 ctatatcaat tccttcatct tcacaaatct ttttcattct aagggtcata tctttaactg
     4261 taactctttt aaaatcgtat ctttggcatc ttgataaaat ggttgcaggt attttgtgag
     4321 gctctgtagt tgctaatata aaaattacat atgatggagg ttcctctaag gtctttaata
     4381 atgcattaaa agctccttga gacaacatat gaacttcatc tattatatat actttatatt
     4441 tagcattggc tggagaatat ttcacacttt ctctaagctc tcttatatca tctacactat
     4501 tatttgaagc tgcatctatt tctacaacat ccattgtatt atcttttaat acagattcac
     4561 atatctcaca ctcattacac ggttcttcat ttattttatt caaacagttt actgctcttg
     4621 aaaaaatctt tgcagtagat gttttaccag tacctcttgt tccacaaaac aaataagcat
     4681 gtcctatatt atcactatat atttgatttt ttaaagtctt aataatatga tcttgaccaa
     4741 taacatcttc aaatttttgt ggtctatatg ctctatataa tgctttatgc atctttttac
     4801 ctctctttag caaaattaat aaaaaaatcc ctcattatct aagggattag tcttttatat
     4861 attaaatgct gtacaccatc tatcgactta attccatagg cgttaccaaa acagttagct
     4921 caacttaggc acccccacgg cacacaaaag tgatcactta ccgctgcttc cttccggatc
     4981 tgacggggtt catgagcttt cattgcgcag gacctaagca tcaacaccac ttatttaggg
     5041 cggccctaca gaaaataagc ctttagatgg aattcaatcc tgctatagcg gattgcaggt
     5101 tacagggcac cgctacctcc ccatctagta cagcaaaatt acaaccatat ttaattactt
     5161 tcattgtttc tggcggagag agtgggattt gaacccacga tacagtttta cccatatact
     5221 cgctttccag gcgagcgcct tcgaccactc gaccatctct ccttaatctc tttttctcaa
     5281 tgccttaaaa aattcttgca atatgatcga gcattcttct tgcaaaacat caaaagctac
     5341 ttttacatta ttattattaa aataatctaa tttaaactca tgctgttttt ctatatatga
     5401 attttttaca tgtcttgtcc ctattataag cttttttatt ctagaattaa ctattgcgcc
     5461 actacacatt atgcaaggtt ccattgtaac atataaatca caatcagtca acctccaacc
     5521 acctaatttt tctgatgctt gttttatcgc taaaatttct gcatgtgccg tagagtcttt
     5581 taacgtttct gtaaggttat gagctctagc tattatttga ttatctttaa ctataatagc
     5641 acctattggt gtttcttttt tattatatgc tttatatgct tcttttaatg cttctttcat
     5701 ataaaatgaa ctttccataa ttcctcccaa aacttataac aaacttatct tatcataatt
     5761 cattttataa ttcaacttaa ttttattttg tttatttaat tattactata tatagcgata
     5821 ttttttttaa ttagttctaa aataattttt agatattcat acaagagtgt tttataaaaa
     5881 tatttagtgt tttatgatga aaattctgct gtgtaaatta acataaaaaa actatgaaat
     5941 taatatttat aataatttca tagttttttt atttattgtt tactcataat atattctctt
     6001 catcaaagca tactctacat atacaattaa tataaattta ttttataaca tcaacaccca
     6061 tataaggtct taattcttct ggaactacta cagaaccatc tgcctgctga taattttcta
     6121 atatagcagc caaacatctt cctatcgcta atccagaacc atttaatgta tgaacatatt
     6181 ctgcttttga ttttttatct cttttaaatc ttattcctgc tcttcttgct tggaaatctt
     6241 caaaatttga acaagaagat atttctacat atctattgta acttggcatc caaacttcta
     6301 aatcatattt aaatgcagct gtaaatccca aatctcctgt acatattttt acaactctat
     6361 atggaagacc taacatttgt aacatagttt cagcatcatg agttaatgat tctaattcat
     6421 tataagaatc ttctggttta acaaatttaa ctaattcaac tttattaaat tgatgttgtc
     6481 taactaaacc tcttgtatct cttcctgctg aacctgcttc agatctaaaa caaggagtat
     6541 atgcacagta tttaattgga agctctgcaa catctaatat ttcattagca tgtatattag
     6601 taacaggaac ttcagctgtt ggtattaaga aatactctaa tccttctatt ttaaacatat
     6661 cttcttcaaa tttaggaagt tgaccagtcc ctataaaact atttctattg gccataaatg
     6721 gaggtaagac ttctgtatag ccatgcttag ctgtatgagt atttaaaaag aaattcatta
     6781 aagatctttc taatctagct cctaatcccc tatataaagt aaaccttgac ccagttattt
     6841 tccctgctct ttcaaaatct aaaataccta gacctgtccc tatatcccaa tgcgctttat
     6901 actcaaaatc aaatttagta ggttctcccc aagttcttat ttgaacattg tcttcatctg
     6961 tttcaccttg tggtacctct ggatgaggaa catttggtat tctcattaat gtatattcca
     7021 tttttgcttc tacttctttt actttctcat caataccttt tatcttatct gataactctt
     7081 tcaatcttgt cttttcttct gttacatctt ttccttcttt tattaattga ggtatttttt
     7141 tagaatttac atttaactca tttttcataa cttcaacttc ttgaagtatt tctcttcttt
     7201 tattatctag ttccactaca gcatcaaggt caaattctct ttcacctctt ctttccatag
     7261 cttttttgat atcatctaga ttttctctta ttcttttaat atctaacata ttaattcctc
     7321 ctaataattt tgataatata aattataatt ccaattaatt tacaagtatt ctagctgtaa
     7381 acttttaatt taaacaaatt tgaaaaataa aaaaagacct ccgtttaccc tataaaaggg
     7441 acgaaaatct tttatccgcg ttgccaccct aattgattat actaaaacgt ataatcctct
     7501 cagacctata acgtaggtaa accgtctagc tttaactaga agctctagag tagattcaaa
     7561 aaccattgta atgagttctc accatccact cattctctga atacaatctg ttttttacta
     7621 ttttccttca ccgcttttta actaattgtt ttatttgata atcattatat tatatatatt
     7681 aacatctttc aatactatta ttcaaatttt tttagaattt tctttaattt tttatattaa
     7741 attcactttt ttacttaatt gaaattctat cttttatcca ttgtatttga attgctcata
     7801 ttattaaata ataacgagga aggaacaaat ggaactttta tattattttc gtcaaattta
     7861 tttttgactt tttccattac atcaaagtaa acatcccagt aatcatcagt tttaacccat
     7921 ggtctagcaa ctatttgcat agtgttgttt cctatagagt ttaaacctat aattggtttt
     7981 gggtcatcca atatcctttt ttcatttata aaaatttcat tcaatatgga cttaactaca
     8041 tcaatatcac tatcatatga aatattatat gaaaaatcta tccttctttt attttgatgt
     8101 gtgtaattaa ttatattgtt acttgtcaat tgaaagtttg gtataattat ggttttatta
     8161 tctggggttt taagaacagt actaaatatt tgtatatcag aaactgtgcc ttctacacct
     8221 gagccttgaa tataatcacc tatattaaat ggcttaaaaa ataaaataat catacctgaa
     8281 cctaaatttg ataatatttc tttaaatgct aaacctatac taaatccagc agctcctaat
     8341 acagctacaa ctgatgtagt ttgtatccct ataacaccca agcttatcaa tgatataata
     8401 gataacatag atatgtatat tccatataca atgaaattat ttacaccttt actcattcca
     8461 tatttgatta aaaaccttga aaccatcttt cttactatct tagatatata gatacctatt
     8521 acaaatacga ttattgcata tataatattt ggtatattat cactaaagtt attaagcata
     8581 ttttctaaag ttcttttagt tatattacta tttgttgaag cagccttagc taatgataaa
     8641 aaattcattt aactcctcct tatgataata ttcctctgtc ctctaattct tttgttataa
     8701 cttctatagt ctcttctttt aatgtctcaa ttgtatgaat atgactacat tcagttagaa
     8761 ctgataactg tttaaattca tttgtctcaa ccttcttcat aaaattatct atatccattt
     8821 tagagtgaat attaagctct gcagttatct gaccatatgt tggatgattt acgatgacat
     8881 ccttaacaat tccacctaaa tctacaataa tctttaattc atcataaatt tctttagaat
     8941 tcctgtgatt cttacatttt atttgctttg taaaatatgg ttgattatat attatatatc
     9001 cttgaggtgt agctattatt tcaaatcctc tagctcttat aagggctata tcttgtacta
     9061 ttacttgcct acttacattt aatatctcag ctagcattcc accttttaca gggcacttag
     9121 attcttttaa aatatctatt aatttgcttc ttctttcatc agaattcata ttacaccttc
     9181 tttctaaatc aaaaacttta cctatactat ataattctat attttatctt attataaatc
     9241 aatattgatt ttatattcta tttaatatta aactttattg caaataaaaa agattacgtg
     9301 gctacgtcct actctcccag gcagtttccc accaagtacc atcagcgcta gagagcttaa
     9361 cttctgtgtt cggaatggga acaggtgtat cctctctgct attgtaacca cataatcttt
     9421 atgcttaata tttct
//
LOCUS       DAMJVX010000133         9391 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMJVX010000133 DAMJVX010000000
VERSION     DAMJVX010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.138"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(100..753)
                     /locus_tag="PC407_002789"
     CDS             complement(100..753)
                     /locus_tag="PC407_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2498217.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKFMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            complement(998..2863)
                     /locus_tag="PC407_002790"
     CDS             complement(998..2863)
                     /locus_tag="PC407_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HDF2498218.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDIPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVDENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2868..5288)
                     /locus_tag="PC407_002791"
     CDS             complement(2868..5288)
                     /locus_tag="PC407_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HDF2498219.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNIDNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYEQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5302..6744)
                     /gene="glgA"
                     /locus_tag="PC407_002792"
     CDS             complement(5302..6744)
                     /gene="glgA"
                     /locus_tag="PC407_002792"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDF2498220.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNRDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6773..7903)
                     /gene="glgD"
                     /locus_tag="PC407_002793"
     CDS             complement(6773..7903)
                     /gene="glgD"
                     /locus_tag="PC407_002793"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HDF2498221.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGS
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7905..9065)
                     /locus_tag="PC407_002794"
     CDS             complement(7905..9065)
                     /locus_tag="PC407_002794"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDF2498222.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKRSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         2970 a         1722 c          912 g         3787 t
ORIGIN      
        1 ttagagtttt tatttgtatt taaagttaaa tttattcttg tttttccttt aatatttaat
       61 agtaaaataa caaaattata aaaaaaataa agagaaactt tataaaagct tctctttttt
      121 catctctgct ataggtatcg ttttccaatg ttctctcaaa actctttcaa cattttctaa
      181 atcattctcc ctcataaatt ttactacttc aatatgttga ttattgctgt atgtcaacat
      241 tttatataac tcatcttcat tatcactagt atatgacttt gacataaaat tatatcttaa
      301 agagttcaat agatttacta acacctgatt atctgattta gttatgtata tatcgtgaaa
      361 ttcattactt aattttaaat agctattgta atttttatac tttatagaga ccgccatcat
      421 ttctgttaat tcttccattt ttactaggtc tgattcagtt aaattttgta atgctgaaga
      481 accggctaac gcatccaata ctccaattac ggaataggtg tctaatttct ccttttcctg
      541 aaattctttt actacaaatc ccttattagg tattttttct agtaaatttt cacctgctaa
      601 cctagttaat gcttctctag ctggtgttct actaactccg atagatttac aaatctcaga
      661 ttcagtaatt ctctgattag cttcatactc tccattttgg attttctttg atatatagtc
      721 atatacttga gttgtaagtg gatctacctt cataagacgt tcctccatat taagattata
      781 aaagttaaat ctccctacaa ctttggtaga gagttcagtt ttatcttact aatttatata
      841 atatcacaat tatgatattt tttatacttc tttgtaatat tttgaaacac taaaattctt
      901 atgataattt atacaaaaaa agtagaaaaa gttttgataa aacaaaactc tttctacatt
      961 caccttattt tctatttatt tttctaaaaa aagatattta atttactcta aaaattctaa
     1021 atgatttaga gtctaaatct actttcaact cattattttc aatttgtata gtactatttg
     1081 catttttaag actatacttt ataggtattt cctcaataaa actactctct atacataatg
     1141 atatactttc cacattttca cttctattta ctaaaattaa cattctatca ttattttcat
     1201 caactctaat aaaagaaaat acagaatcat tattagtata tataaattct gtatttccag
     1261 aacttaaaag tttattctta tttctaattt ttataacatc tctataaaaa ttaagcatat
     1321 cttcgtcttc atttttccat ggataagttc ttctattatc tgggtcaaca cctccacaaa
     1381 gacctgcttc atctccataa tatatatatg gtactccttc aaatgtcatc tgtatagcta
     1441 cagcaagctt cactaaatct acatcatcat ttaattccgt tttagctctt gtaacatcat
     1501 gtgttccaat taaatttaaa ttagacctaa atgattcttt tggataattt tctttaatct
     1561 gcatatatct attacaaagt tcatatgaat taatttctcc ttttaagaaa gaaaacatgt
     1621 tatttctaaa aggataaccc ataacagaat ctaattcttc acctaataaa taacttcttc
     1681 tctgaccata gctaatctta tttgatgcat cttcccatac ttcacctata agaattgagt
     1741 catcatctgc cttttttaat tcctttttta actctcttat aaacttagtt ggtaattcgt
     1801 ctgcaacatc tagtctccag cccttaatac ccatatttac ccatttattt ataacgctat
     1861 cattgtcata tattatataa tccatatagc tatgttctaa ttcatttata tttggcaatg
     1921 tcttaacatc ccaccaactt ttatactttt gaggaaattc ttcaaacata taccatgaat
     1981 aatatggaga ttctttagat tgatatgccc ctaaactatt atagtttcca tacatattaa
     2041 aatatttact atctgctcca gtatgactaa aaactccatc taatactatt gatatacctt
     2101 tttcttttgc tttatcaata agttctttaa atgtatcttc atccccaaac attgggtcaa
     2161 tttttttata gtcacctgtg tcatatttat gattactaga agcttcaaat atagggctta
     2221 gatatagtat acttacacct aattttttta aatatccaag tttattgatt atccctctta
     2281 aatttccacc atggaaatcc catcttataa catccccttg actatccttt atgtacatag
     2341 gtatatcttc ccaatttcca tatatgaaac tatttttttt aggattatca accttaccac
     2401 tacgatttcc attattgaat ctatctacaa atatatgata aagtattcct tctttgtacc
     2461 atgttggtac tttaaaatct ttgtaaacag tgatttgata tttattaata tcactataat
     2521 tatattcaca agaatatcca ttatctctgt tttttccata aaataaagtc tttatctgac
     2581 catccatttc aacatcaact ttaaagtaat aaaaataaac acctgtacca tcaaactttt
     2641 caatcttacc acaaaagcac tttccaagtt tatcattatc taattcttca tttaaagtta
     2701 ttgttctcat ttcttcttct ctatttattt ctagggaaat agacttgata ttgtatccct
     2761 catttacttt aacacaaatg gtcaattcct catcaaattt taaagctcca aacggagcct
     2821 taaaattttt atcccaagag ttatattcta ttacactttg catttaatca caccctttta
     2881 aaccaaatgt cttttgcata ctcagaaatt gttctatcag aagaaaaaaa tccagagtta
     2941 gctatattaa ctaaagacat tttattccat ttttctttat ctctatataa cctgtttata
     3001 tctgcttgag cttgaccata attttcaaaa tctctcaata caaaatactc atcattgtaa
     3061 gtagtgagtg aattataaat acttcttcct tcttcgccaa gattaggaat aaatccattt
     3121 attaaatcat ctaccaccct ttttatatcc atattagaat gatataagtc aagtgaagaa
     3181 tatccaccat atttattata atttagtact tgttctgcac taagtccaaa taaaaacata
     3241 ttttctttac caacctgttc acatatctct acatttgcgc cgtcaagtgt agccaaagtt
     3301 atagcaccat tcatcataaa cttcatatta cttgttccag atgcttcctt agttgtagtt
     3361 gaaatttgct cactaacatt tgctgctggt attattattt ctgctaatga tactccataa
     3421 ttctctaaaa atactacttt taacttgtcc tttactctaa catcattatt tatagtactt
     3481 gcaacagaat ttataaattt aattatacat ttagccaaat aatatcctgg tgctgctttt
     3541 gcaccaaata taaaagttct tgggtcaaga ttcaaattag gattatcaag taattcatga
     3601 tacatatgaa gtacattaaa tatattcagt aactgtcttt tatacgcatg caatctctta
     3661 acttgaacat caaatatcga acttgggtct acatttaaat cgtatttatc ttttataaac
     3721 tttgctagat ttgctttatt attgtatttt atatcatcaa gtctttgtaa aacagaggaa
     3781 tcatttttaa atttttcaat attttttaat tgtaaggtat ccttcttcca tgaatcacca
     3841 attaaatctg taattaaatt gctaagttga ggatttgaac ttataagcca tcgcctatgt
     3901 gcaattccat tagtcttatt attaaattta tttggttcat cttgatagaa atcttttaat
     3961 acctctgttt caagaatatg agtatgaagc ttagcaactc catttacact atgacttgtg
     4021 acaatacata agtttgccat attgatattg tcacagtcta ttatactcat tctttttatt
     4081 ctatcctgct catatccttt attatttaat tcctctacat atcttctatt tatttcttct
     4141 attatcatat aaattcttgg taatagttct ttcatcatac ttacaggcca tttttccatt
     4201 gcttctgaca ttatagtatg attagtatag gacactgttt ttttagttat ttcccatgct
     4261 tcatcccaag ataaattttc ttcatcaagc aaaattctca taagttctgg tatgcataag
     4321 gtaggatgtg tatcatttat atgaattgct accttatcga ataaattatc aatatttaat
     4381 ttgttctttt tatattttct tattatatct tgtatccctg cacttacaaa aaagtattct
     4441 tgtttcaacc tcaacaactt acctgcatag tttgaatcat caggatataa tacctgtgat
     4501 atttcctcag tataatactt atgttttaaa gcctcttcat agctaccaga ataatttaga
     4561 gcatttgagg taagttcacc aaaatctctc tttggtattt cactcttaaa caatcttaaa
     4621 gtatttatac attggttttg gtatccgatt attggtatat cataaggcat tgccattaca
     4681 ggaaggtagt ttttatgaat tacttttaaa tgactacctt ccttgattaa ttctacctca
     4741 ccaccaaact tcaccattgt tgcttcattt ggtcttacag tttcccatgc atatcgtcct
     4801 tctgtaagcc agttatcagg cacttctacc tgataaccat ttacaaattt ttgttcaaac
     4861 agaccatatt tataccttat tccatatcct tgaccactta tgttcaaaga tgccatagaa
     4921 tctaaaaagc aagctgctaa tctaccaagt ccaccatttc ctaaggctgg gtctacttca
     4981 gcttcaatta aatcatccaa gttgattcct aattcagata aaccttctct aacttcttct
     5041 tcaatattta aatttaataa attagatttt aactgtctcc ctattaaaaa ttctaaacta
     5101 aaataataaa cttctttttt cttatctaac tttgtagcaa cccatttttt tgctatctca
     5161 tctttcaata gactacataa tacatttaac aattgttcat cagtagcttc cttgatagat
     5221 tgtgcatata agctatacat ttttacttca aatttttgct tgaatttctt tttgcttatt
     5281 gttaccaaaa aaaccactcc tttaatcaca tatgtctcta tatgtttcta tatactcctt
     5341 agctgatttt ttccaactat tatctgtttt cattgcattt tcaactaatt taatccattt
     5401 ttctttgtcc ttaaatactt tgattgcatt tttcaaacag aaaaacatct catgagcatt
     5461 gtaattttta aaactaaatc cattgccttc tccagtaaat ttattataag gtgttactgt
     5521 atctctaagc cctccagtct ctctaactat tggtaaagaa ccatatctca tagctaacat
     5581 ttgacctata ccacaaggct caaataatga tggcattaag aacatatcac ttgcagcata
     5641 tatctgttga gccaatgatg cattaaaagt aattctagct gatactttat ctgaaaaatt
     5701 agaatcgtaa tagttgaaca ttgattgata ttgatgttct ccagttccta aaacgacaat
     5761 ctgtaaatct tctctcataa gttctggcat catgtatgct attaaatcaa atcctttttg
     5821 agaaactaat ctcgaaacta tacctatcaa cggaatagat gaatcttttt taagtcccaa
     5881 tatatcttgt aatctcaatt tattttctac ttttttgtca attgagtcta catcataatg
     5941 aacaaatata tctttatctt ttgcagggtc gtttaaatca taatctatac catttaatat
     6001 accttttaat tttcctgact cttttctaag taatccatct aattgttcac cataaaaaga
     6061 tgtttgtatt tcatttgcat aagtaggact aacagttata attttatctg caaaatttat
     6121 accagctttc ataaaattta ttgagccata atactctata tctctattat caaagtattt
     6181 ttgtggtaag tccaatatat catataaaac atgcttatca aatacacctt gatattggag
     6241 attatgtata gtatacatag tttttatatt tttatacccc tcaagggttg catatctttc
     6301 ttttagaatt aatggtaaca tccctgtatg ccaatcattt atatgtatta catctggata
     6361 aaaatcaatt ctacttatag cttctaaaac tgcatctgta aagaaagtat atctttctgc
     6421 atcatctcca tatccataga tactctttct ttcatcttct cttctaaaat aaaattcatt
     6481 atctataaag taaaatttta caccatccaa ttccatttct aaaagaccac agtattgatt
     6541 tctccatcca acacgtacac taaacacagc tatttctttt agttgatctt ttaaataact
     6601 tggtatagtt gaatatttgg gcattattac ccttacatct acaccttctt tttttaattc
     6661 tttaggcaat gcataagaaa catctcctaa tcctccagtt tttgcaaagg gccaacactc
     6721 tgctgttaca taaaaaacct tcattagtca ttcccccttc taaaaaattt atttatatat
     6781 ttgtattctt ttccactaca agaggatatt tttcatctcc tcttaattct ttcccatttg
     6841 atacactcgt atttttatct aatattgcat tatatagttt tgcatttgac tttattacac
     6901 aattttgcat tactattgag ttctctataa ctgttccttt ttcaacattt acctttctaa
     6961 atatgatact atcttttaca gtaccttcta ttaaacaacc agatgctata aagctatttt
     7021 ctactttagc actatctgta taaattgtag gtgattcatt cttttctttt gtaaatatct
     7081 ttctttcaga gtataataat tcatctgcaa cttctaaatt taataaatct ttgctcattt
     7141 caaaatatga ttcaacagaa tttacacatt ttagatatcc tgtatattca taagctccta
     7201 ttttcatatt ttctgcttct tcaataataa attcttctaa ataacaataa ctgcctttat
     7261 ttacaatgtt atatatacat tcaacaaaat cttcacgctt cataatatat gtctccatac
     7321 atatgtttgc tctagggaat ttgcctaaat tatttcctac atttattacc ctattgttac
     7381 tatctaaatt tagtgttaag ttaccagcaa aatctttatt tgcattattt acatttttat
     7441 atattatagt tatatcatta tgagattttt tatggtattc caatgcttga gaataattta
     7501 aattgcatag catatagctt ggagctatta aaatatattc ttcttcactc ttttctatat
     7561 aatctagatt agctaagata gtatatatat caccttctct atgtgagtaa gtatttctat
     7621 acttagtgtt ttctggacta aaaattgata ggccaccttt ttttctactt aaatcccaat
     7681 ctttaccact acctatatgg tctgtcaatg atctgtattt ttcttttgcg aatattccaa
     7741 ctttagtgat tcctgaattt atcatattag ataaagcaaa gtctattatt ctatatcttc
     7801 cagctattgg tgtagatgct attggtcttc catagttaag tttgtttatt gctgggtctc
     7861 cttttttgtt taaatttatt atccctaaac attcatttct catcttaaag acctccttct
     7921 attaatacgc atctaggttc gatattttca tactcagaaa ctacatttat ttcattattt
     7981 acattcttta tctgaacatt atcatttata gttgcacact cacctatcat agctttttct
     8041 atagttacat tttcacctat tacaacattt ggcattatta ctgaatcaat tactttactt
     8101 cctttaccaa caacaactcc atgtgataat acagagttgc ctacttcacc taaaattcta
     8161 caaccatcag ctatcattga tctatgaaca tttgcatttt tacctatata ttgtggaggc
     8221 attgccatag tatttgtata tatcttccat ttagggtctg ctaaatcaag tgtttctgga
     8281 gattttatta tatccatatt agcatcccat agactttgta tagttcctac atctctccag
     8341 tatcctttaa atggataagc atacattcca acattatctt ctaacatttt aggtattaaa
     8401 ttttttccaa aatcatcata atttatttct ggattttttt ctgcttcctt aaaataattt
     8461 ctcaacattt tccaatcaaa tatgtatact cccatagatg ccaaattgct cttaggttca
     8521 cttggttttt cttcaaattc atagatactt gaatcttcat ttgtattcat tatcccaaat
     8581 cttgaagctt catcccaagg aacttcaatc actgctattg tagctttaga gcgtttttcc
     8641 ttatgaaatt ttaacatttt gctataatcc atcttgtata tatggtcacc agataatatc
     8701 aatacatatt ctggattata ggtatctaca aagtccatat tttgatatat cgcatgtgct
     8761 gtaccattat accaatttcc ttctttttca ttcataaatg gttgtaaaac atagacacca
     8821 ccatttattc tgtccaaatc ccaatgactt cccatgccta tatgtgaatt taatatcaac
     8881 ggtctatatt gagttaaaac acctacagta tcaataccag agtttgaaca attacttaac
     8941 acaaaatcta taattctata cttacctcca aatgatacag ctggtttggc aattcttttt
     9001 gtaaaaacac ctaatcttga accttgccct cctgccaaaa tcatagctaa catctctttt
     9061 ttcatttaat gacactcccc tcatctagta attaatattt ataattgcat ttttactttt
     9121 ataaacttta tcagtcaata aatgccgtgt ataaatgttg cttaaataat accttaataa
     9181 aaataaaata atatcaacta tacaattgat ataatgtata ccccataatg aacttaccga
     9241 cgtatacatt attttccata agcactatag tatttattaa tattcaatta tcggttaaaa
     9301 aataatacaa attttatttt ataaatataa aaacattttt tataaatccc ttgtgtcata
     9361 taaacatgct tattttttaa tttgttaaat t
//
LOCUS       DAMJVX010000134         9357 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.139, whole genome shotgun sequence.
ACCESSION   DAMJVX010000134 DAMJVX010000000
VERSION     DAMJVX010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.139"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            67..1827
                     /gene="prdR"
                     /locus_tag="PC407_002795"
     CDS             67..1827
                     /gene="prdR"
                     /locus_tag="PC407_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HDF2498223.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            2256..4136
                     /gene="prdA"
                     /locus_tag="PC407_002796"
     CDS             2256..4136
                     /gene="prdA"
                     /locus_tag="PC407_002796"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HDF2498224.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            4217..4501
                     /locus_tag="PC407_002797"
     CDS             4217..4501
                     /locus_tag="PC407_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498225.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            4539..5264
                     /gene="prdB"
                     /locus_tag="PC407_002798"
     CDS             4539..5264
                     /gene="prdB"
                     /locus_tag="PC407_002798"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:4989..4991,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HDF2498226.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            5344..6102
                     /gene="prdD"
                     /locus_tag="PC407_002799"
     CDS             5344..6102
                     /gene="prdD"
                     /locus_tag="PC407_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HDF2498227.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            6117..6584
                     /locus_tag="PC407_002800"
     CDS             6117..6584
                     /locus_tag="PC407_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF2498228.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            6602..7084
                     /locus_tag="PC407_002801"
     CDS             6602..7084
                     /locus_tag="PC407_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF2498229.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPAVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            7104..8111
                     /locus_tag="PC407_002802"
     CDS             7104..8111
                     /locus_tag="PC407_002802"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HDF2498230.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            8231..9112
                     /locus_tag="PC407_002803"
     CDS             8231..9112
                     /locus_tag="PC407_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2498231.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         3665 a         1148 c         1880 g         2664 t
ORIGIN      
        1 taatatgcta gaataaatat atataaataa gttttgatga ggtactacta taaagggggg
       61 gactttatgt tttcgatacc agaagtaaaa aaagtagaag aggtaatgga tacaaaattt
      121 acaactatag acgaagacac aagaattgaa gatgcaataa aagaaatgat aaaaagcaac
      181 acaaaaactc taatggtaat agattcatct gaccaattaa aaggcattat atccatgacg
      241 gatatacata atctttatga gatgcataaa aagtatgaag gtcaaccagt aaaacttata
      301 atgaagaagg atgtaatata tgtcaacgaa ggtttaacct tagatgaatg tagagatata
      361 atgatattaa aaaatatagg gattcttcct gttcttagag ataataaaat tattggagtt
      421 ctaaaacaag agcacattag ggactatcta tatatgcatt tagaagatta tggattgact
      481 ttgaagtata ttataggtca aattaaagaa ggaatttgtg ctataaataa cgaaggtgtt
      541 gttatcttgt ggaataagtt tatggaggaa aggtatgata taaagtctga agatatagtt
      601 ggtcgaccga tgaatgaatt tttggaaaat actatttcag aaaaagtact aaatagtaaa
      661 gttggaatga gtgacttgta ttttacagat aaaaaagaaa atatgtatgc attagttcat
      721 gcaaatccta tattttataa agaagaattt atcggtgttg tatgtacaga agttgatgtt
      781 acagaagcta agatattagc tcttgaactt gaaaaagtaa atgatacatt aaaatatcta
      841 aagaacgaag tcaaaaattt atctaaaggt agttttgata agattttagg taaaagttat
      901 aaattggaaa aatcgaaagc gattgcaaaa caagtggcta gaacaaatag tagcatattt
      961 atttggggtg aaagtggtac gggtaaagaa gtatttgcaa gagctattca tgattatagt
     1021 gagagaaaag gtcaatttat acctgttaac tgtagtgcca taccaaatga gttatttgaa
     1081 agtgagtttt ttgggtatga atcaggtgca tttacaggag caagtaaaaa gggtcgaata
     1141 ggtatatttg aacttgcaaa agatggaact gtatttttag atgaaattgc cgatttacct
     1201 cttagtatgc aagcaaagtt gcttagagta ttacaggaaa aagaaataag aagagttggt
     1261 ggagatacca caataaaaat aaatcctaga ataatatcag ctaccaataa agatttgagt
     1321 aaaatggtaa aggcagaaaa atttagagaa gatttatact atagattaaa tgttgttgaa
     1381 ataaagattc ctccacttag agagagaaaa gaagacatag gattattggt tcatagcttt
     1441 ttagaggaga tatgtaaaca aaataataag cctgttctta caattagcaa agatgtaata
     1501 gatatatttc agaattatag atggaaaggt aatattagag aattaaaaaa tacgattgaa
     1561 aatattgttg tattgtcaca aaattctaaa atagatgtag atgatgtacc aagttatatg
     1621 atggattcta caaataatag tactgaagaa gaagagtatc ccttagattt aacgaaggca
     1681 actcaaaaaa ttgaaataaa gaatataacc aaggcactta aaatgtctaa tggaaataaa
     1741 gctaaagcag ctaagatatt aaatataccc agaacaactt tgtattataa gatagaccaa
     1801 tacaaaatag atgtgtctaa aatatgacat atgactaaat tttgacaaaa tgataaactt
     1861 taatttaaaa cctagtaaag ccaacacttg tataagtgtt gtgtatatag atgtgttaaa
     1921 aaaaatgaaa aagtgttgat taattgacac tttttttgtt tgagaaaatg tttacaaata
     1981 caaaaaataa catattttaa ataaatattt atataatatt tggctaaaaa tagcgataga
     2041 tgaagaatct attatagaaa aaatcttttt gttaataagt tggcatagga attgcttata
     2101 atataaagtg actttgagtg agaaaaaaat aatattaatc ataacaaatt atgtaggttt
     2161 taatttactt aatttctcag ttaaggggtt acctgttgag gaattgagat ttaaaataat
     2221 aaataagaaa ataatgtaag gaggtatttt atactatgtc aataacttta gaaacagctc
     2281 aagcccatgc aaatgaccca gcagtttgtt gttgtagatt tgaagcggga acaattatag
     2341 cgccagaaaa cttagaagat ccagcaatat ttgcagactt agaggattct ggattattaa
     2401 caataccaga aaatggatta actataggtc aagtactagg agctaagtta aaagaaactt
     2461 tagatgcact ttctccaatg actacagata acgtagaagg atacaaagca ggagaggcta
     2521 aagaagaagt agtagaagaa acagtagaag aagcagctcc agtatcagaa gcagcagtag
     2581 ttccagtaag cacaggagtt gcaggtgaaa cagttaaaat acacataggt gaaggtaaga
     2641 acataagctt agagatacct ttatcagtag ctggtcaagc aggagttgct gctccagtag
     2701 caaacgttgc tgctccagtg gcaagtgcag cagcagaagt agctccaaaa gttgaagaaa
     2761 agaaactttt aagaagctta actaaaaaac actttaaaat agataaagtt gaatttgctg
     2821 atgaaactaa aatagaagga actactttat acatcagaaa cgcagaagaa atatgtaaag
     2881 aagctaatga aactcaagag ttagttgtag atatgaagtt agaaataata actcctgata
     2941 aatatgaaac ttacagtgaa gctgtattag atatacaacc aatcgctact aaagaagaag
     3001 gcgaattagg ttcaggtata actagagtta tagatggagc tgtaatggta ttaactggta
     3061 cagatgaaga tggagttcaa ataggtgaat ttggttcttc agaaggtgag ttaaatacta
     3121 ctataatgtg gggtagacca ggtgctgctg acaaaggtga aatattcatc aaaggtcaag
     3181 taacaataaa agcaggaact aacatggaaa gaccaggacc tttagctgct caccgtgcat
     3241 ttgactatgt aactcaagaa ataagagaag cattaaagaa agttgacaac tctttagtag
     3301 ttgatgaaga agtaatagag caatacagaa gagaaggtaa aaagaaagtt gttgttataa
     3361 aagaaataat gggacaaggt gcaatgcatg ataacctaat attaccagtt gagccagttg
     3421 gtacattagg agctcaacca aacgttgact taggaaacat gccagttgta ttatctccac
     3481 ttgaagtatt agatggtggt atccatgcat taacttgtat aggacctgca tcaaaagaaa
     3541 tgtcaagaca ttactggaga gagccattag taataagagc tatggaagac gaagaaatag
     3601 atttagtagg tgttgtattt gttggttctc cacaagtaaa tgctgagaaa ttctatgtat
     3661 ctaagagatt aggtatgtta gttgaagcta tggaagttga tggagctgta gtaactactg
     3721 aaggtttcgg aaacaaccat atagatttcg catctcacat agagcaaata ggtatgagag
     3781 gtataccagt agttggtgta agtttctcag ctgttcaagg tgctctagtt gttggtaata
     3841 aatacatgac tcacatggta gacaacaata agtctaagca aggtatagag aatgaaatat
     3901 tatctaacaa cactttagct ccagaagatg ctgttagaat aatggctatg cttaaaaatg
     3961 ctatagaagg tgtagaagtt aaagctcctg aaagaaaatg gaatccaaat gttaaattaa
     4021 ataacataga agctatagaa aaagttacag gagaaaaaat agtattagaa gagaatgagc
     4081 aatctctacc aatgagtaag aagagaagag aaatatacga aaaagacgaa aactaaaatt
     4141 tagatactaa ctaagattta gacaataaca taaatttaga tccaagttta aaacagtata
     4201 gagaggtaga attataatgg ataggttaaa atatatctca tcagagactt tttatgaagg
     4261 tattatagtt gacatcaaag gtggtggagt gactatagac ctaaaaggta gacttggaca
     4321 atttaaaata cctaatagaa tgcttataac tgactacgaa cttaagatag gtcaagaagt
     4381 tggattcatg ctaagctatc ctgaggtatt aagtccagag cctaatcaag agtatgtaga
     4441 aaatattagg agagaaaaag aaaaacaagc agaaatgcaa tcaaaacaag aagaaaaata
     4501 ataaataatc gtttgaaaat agaagggaga ggaaatatat gagccttaca acagtacaag
     4561 gacttcaatc tgaaatattc gttccaataa cacctcctcc agtttggact cctgtaacta
     4621 aagaattaaa agatatgact atagctttag ctacagcagc tggtgttcat ttaaaatctg
     4681 acaagagatt taatctagca ggagatacta catttagagc tatacctaat acagctacag
     4741 ttgatgaaat gatggtatca catggtggat atgacaatgg agatgtaaac aaagatataa
     4801 actgtatgtt ccctatagat agattacatg aattagctgc agaaggattt atcaaaggag
     4861 tagctcctat gcactatgca ttcatgggtg gtggaggaaa ccaacatgtc ttcactgaag
     4921 aaactggtcc tgctatcgct gctaaactta aggaagaggg agtagacggt gtagttatga
     4981 cagctggctg aggtacttgc catagaactg ccgtgatcgt gcagagagca atagaggaag
     5041 ctggaatacc tacaataata atagcagctc ttcctccagt agttagacaa aacggaactc
     5101 ctagagcagt tgctccatta gttccaatgg gtgctaatgc tggtgaacca cataatatag
     5161 aaatgcaaac acatatatta agagatacat tagagcaatt agttgcaata ccatctgctg
     5221 gtaagatagt tccattacca tacgaatata aagctcacgt ttaaaatgta atttatattt
     5281 gaaaatatag aacccttctc ctacacggtt aggagagggg ttttaagaac aaaggtggat
     5341 attatggaag aaaaaataat gagaagactt gttataaaac cgtttcatat gaatgaagtt
     5401 aatttcggtt caaaaacttc tataaagaaa gatgtactta caatagattt atcaagtata
     5461 gatgaaataa aagaaagaga agagttaata acagacataa aagtagacat tataaaacct
     5521 ggagattatg atagagaaat taatactatt atggacatta taccaatatc aacaaaagtt
     5581 ttaggcagac tgggagaggg tatcacacat actttaacgg gtgtatatgt aatgttaact
     5641 ggtgcagatg aagatggaaa tcaaatgcat gaatttggtt cttcagaagg aattttgaaa
     5701 gaccaaatgg tatttgggag atatggaact ccatcagttg aagattatat aatccatgta
     5761 gatgtaactt taaagggagg tttacctttt gaaagaacac ttccacttgc agcattcaga
     5821 gcatgtgatg attttataca agaaataaga gcgtctttga aaatggaaga cggaagaaat
     5881 gctactcaag ttcgtgaata ttttgacaaa attcgaccaa atgcaaaaaa ggttgtcata
     5941 gtaaaacaaa tagcaggtca aggtgctatg tatgacaatc agttattctc aaaggaacct
     6001 agtggatttg aaggaggaac atcaattata gatatgggaa atgtacctat gattatatct
     6061 cctaatgaat acagagatgg tgcccttaga gctatgactt aagagaggtg gatattatgg
     6121 gaataggacc ttcaacaaaa gaaacatctc ttcatcactt tagagaccca cttttagaca
     6181 tagttgaaag tgataaggat gtagatttac ttggagtaat agtagtagga actccagatg
     6241 gaaatgaaaa taaaaccttt gtgggacaaa gaacggccgc ttggctggaa gccatgagag
     6301 tagatggagc tatagtttca tcagatggtt ggggaaactc acatgttgac tacgctaaca
     6361 catttgagga aataggaaaa agagatatac ctgtagttgg agttacattt aatgggactc
     6421 aagctaagtt tgttgtaagc aatcaatata tggataccat agttgatatg aacaaatcaa
     6481 aggaaggtat tgagacagaa gtagttggag aaaacaacac aaatgaaata gatgctaaaa
     6541 aagctttagc atttttaaaa cttaaaatga gaaaacatgg ataaaaaatt taagtaaggg
     6601 aatgagtttt gtgggaattt ctacatcaat gaaaaacaca tcacttcata tatttagaga
     6661 cccgttacta gatttgatag ataatgattc tgacatagac ttgttaggag tggttgtagt
     6721 tgggacatca gagcacaatg aatggaaaac atttttagca actagacttg gaagatggat
     6781 tgaatcattg cgacctgatg gagtgattat aactttagat atggcaggaa accagcacat
     6841 tgattttaca aatgctatag cagagtttgt aaaatcagat atacctgcag taggtcttac
     6901 tattatggga gcagatggat tagtaattac caatccatat ttggataaag ctaccataat
     6961 tgattataaa aagacaacag gctacataga aacagaagta gttggagaca atcacatgga
     7021 tgaagttgat gttaaaaaag cattggcttt cctaaaatta aaaatgcgta aagacgcaaa
     7081 ataaacctaa gggggaattt gatatgaaat ttagcagaag tatacaagct atagactctc
     7141 atacagcagg tgaagcaact agaatagttg taggtggaat acctaacata aaaggaaatt
     7201 ctatgcctga gaaaaaagaa tatttagagg aaaatctaga ttatttaaga actgctataa
     7261 tgttagagcc aagaggacat aatgatatgt ttggttctgt aatgactcaa ccttgttgcc
     7321 cagatgctga ctttggaata atcttcatgg atggtggtgg ataccttaac atgtgtggcc
     7381 atggtacaat aggagctatg acagcagcta tagaaacagg tgtagttcca gcagtagagc
     7441 ctgtaactca tgtagttatg gaagctccag caggaataat aagaggagac gttacagttg
     7501 tagatggaaa agctaaagaa gtatcattct taaacgtacc agctttctta tataaagaag
     7561 gtgttgaagt tgacttacca ggtgttggga ctgttaaatt tgacatatca tttggtggaa
     7621 gcttctttgc tataatacat gcaagtcaat taggtttaaa aatcgaacct caaaatgctg
     7681 gtaaattaac tgaactagct atgaaactta gagatataat aaatgagaaa atagaaatac
     7741 aacatccaac tttagctcat ataaaaactg tagacttagt tgaaatatat gatgagccaa
     7801 ctcatccaga agctacttac aaaaatgtag ttatatttgg tcaaggtcaa gttgacagat
     7861 ctccatgtgg aactggaaca agtgctaaat tagctacttt acatgctaaa ggtgaattaa
     7921 aagtaggaga aaaattcgta tatgaaagta tattaggaac tttattcaaa ggtgaaatag
     7981 ttgaagaaac taaagttgca gatttcaatg ctgtagtacc taaaataact ggttctgctt
     8041 atataactgg atttaatcat tttgtaatag atgaagaaga cccacttaaa catggattta
     8101 ttcttaaata agtaataaat aaattaggaa attaaaatag aatagtagcc acttgtcggt
     8161 tgctattcta tagcttttag gctataagat tcagaatata tgaaaaaata aaaaaatagg
     8221 aggatggatt atggtagcaa tcttaaaggc attatatgta gtattcgcag caatctttgc
     8281 agttgtgttt ggtaaagacg tagctaaagc aaacaaagag gggaaactag aagaacaaag
     8341 tactccaaaa gttgctgtaa caggatttat aacagacttc tttgatacat taggaatagg
     8401 gtcatttgct ccaacaatag caatgtcaaa ggctttaaag ttaaatatac cagataaaaa
     8461 aatgcctggt acattaaacg tagcacatac tattccagtt gttacagaag cattcatatt
     8521 tacatcaatc ataccagttg atggtgttac tttagtatct atggttgtgg cagcagcagt
     8581 tggttcatat ataggtgcag gaataatagc taagatggac gagagaaaaa tacaacttgt
     8641 tatgggtgta actttagcat taacagcaat attaatgtta ttaggtcatc cttggattaa
     8701 cgtattacca ggtggaggaa atgaattagg tcttactggt gttaaattgg taataggtgt
     8761 aataggtaac tttatactag gagctttaat gacagctggt ataggtctat atgctccagg
     8821 tatggcaatg gtttatttct taggaatgtc tgcaaaagtt gcattcccaa tagttatggg
     8881 ttcttgtgca ttattaatgc cagttgcaag tatgaaattt ataaaagaag atgcatatac
     8941 taaaaaagca tcagttataa tagctatatg tggtttaata ggagttttcg tagctgcata
     9001 tatagtaaaa actctaccaa tggatatatt aaaagcatta gtaatagtag ttatagctta
     9061 tacttctgct acaatgataa tggctgcaaa taaaaacaaa aagactgctt agttaaagac
     9121 tacttagtgt ttattaaata ttacagctaa taataaagat tataaactgc tttaaaatat
     9181 tgaattaata ttttagggca gttttttatt tgctaaatta atataaagca attaacatgc
     9241 caattatgta atatatatag tgatttagta gatttaatgt aaaaaacgtt gtattttcaa
     9301 agaataaagc ataatttaaa gctaaatatt taaattttat aatcaataag ttcaata
//
LOCUS       DAMJVX010000135         9160 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMJVX010000135 DAMJVX010000000
VERSION     DAMJVX010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9160
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.140"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            127..1755
                     /gene="aspD"
                     /locus_tag="PC407_002804"
     CDS             127..1755
                     /gene="aspD"
                     /locus_tag="PC407_002804"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HDF2498232.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDEI
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1876..2871
                     /locus_tag="PC407_002805"
     CDS             1876..2871
                     /locus_tag="PC407_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDF2498233.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGIGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3029..3697)
                     /locus_tag="PC407_002806"
     CDS             complement(3029..3697)
                     /locus_tag="PC407_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2498234.1"
                     /translation="METVSNNQSSVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(3796..4108)
                     /locus_tag="PC407_002807"
                     /pseudo
     CDS             complement(3796..4108)
                     /locus_tag="PC407_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431045.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4535..6955)
                     /locus_tag="PC407_002808"
     CDS             complement(4535..6955)
                     /locus_tag="PC407_002808"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDF2498235.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(7309..7659)
                     /gene="rsfS"
                     /locus_tag="PC407_002809"
     CDS             complement(7309..7659)
                     /gene="rsfS"
                     /locus_tag="PC407_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDF2498236.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(7776..8348)
                     /gene="yqeK"
                     /locus_tag="PC407_002810"
     CDS             complement(7776..8348)
                     /gene="yqeK"
                     /locus_tag="PC407_002810"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDF2498237.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSRNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(8349..9038)
                     /locus_tag="PC407_002811"
     CDS             complement(8349..9038)
                     /locus_tag="PC407_002811"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDF2498238.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         3016 a         1544 c         1213 g         3387 t
ORIGIN      
        1 agttattatt ttaatttcat actttacttt ttctataaac ctattataat taaagtataa
       61 tcatttttgc aacttgttta ttttattata aaaatgtgaa acttattaca taaggaggaa
      121 tttaacatga aaaatcatac ttataaagaa atcaaaaaca tatatggaaa aataagccct
      181 tttgaattta aggacaaatt aatagatatt gcaaaataca ctgcaaaaga aaataacaga
      241 gaattacttg atgcaggtcg tggaaatcca aactggactt gttcaactgc tagagaagcc
      301 ttttttactt ttggtcattt cgctattact gaaactcgtt ctaattggga tttaggtcat
      361 ttggctggaa tgcctcaaaa aaagggtatt aaagaaagat tttttaaatt tataaatgaa
      421 aatatagata tgcctggagc atatttagct agagatatta taaactttgg tataaatgaa
      481 cttggatttg atggagatga atttgttcat gaactagctg atggaattat tggtgataac
      541 tatccacttc cagatagaat gttacctcat atggaaaaaa tagtacacga ttacctagtt
      601 caagaaatga aatatgatat atcaggtaaa tatggtgatg tagaaatttt tgctgttgaa
      661 ggtgctacag ctgctatgtg ctatatattt gattcattaa tggcaaatga acttctaaaa
      721 aaaggagata caatagcctt aatgactcca atatttacac cttatcttga aatacctaac
      781 cttcctcgtt acgactttaa agtggtaaat ataaatgcta atgaagttga tgaaaaaggt
      841 gctcatacat ggcaatatac aaaaaaagaa ttagagaaac ttcgtgacaa atctattaaa
      901 gctttattcg ttgttaatcc taataatcct gcttctatag ctatggatga aatatcttgt
      961 aataatctaa tagatgtaat tgaaaattat aataaagatt taatgataat atcagatgat
     1021 gtatatggaa cttttgtaga agatttttca tccttaatgt caaaactacc ttacaacact
     1081 gttggagtat attcttattc aaaatatttt ggagttacag gatggagact tggtacattt
     1141 gcccttcata agaaaaatgt ttttgataag aaaataaatg atttaactgg agaattaaaa
     1201 aaatcagtag ataagcgtta cagtgatatg agtcttaacc cttctagtct ttcatttatg
     1261 gaaagagttg ttgctgatag cagacttgtt gctctcaacc atactgctgg tctttctact
     1321 ccacagcaag tacaaatggc gtttttctct gcttttgcat tgattgacaa agtagatgcc
     1381 tacaaaaaac tcaatatgag tatctgccat agacgtcaaa aactattatt tgaagcttta
     1441 gagcttccaa ttaatgaaaa caaaaacaat gctgcatatt acactcaatt tgatattgaa
     1501 gaatgggcta aattaaacta cggagaagac ttatttaaat ttataagtaa aactgcatct
     1561 ccagtagatg ttctttataa attagctaat gactattcag tagttttact tagtggaaat
     1621 ggattctatg gccctgaatg gtcaattaga atatctctag caaatctata tgatgaagct
     1681 tatactaaga taggaaaagc cataagagag atattaaatg gttatatcgt tgaatggcaa
     1741 aaaattaaga aataattata ttacactgaa atatctaaat tatatgaata aaagagttta
     1801 tgtagacctt aactactttt tttgatataa tcctatcgga attataaatt ttatttatga
     1861 aaggcagaga atcgtatgag tattaagaaa aaagttgcca tagtttttac tggtggaact
     1921 atatctatga cagtagatga taaagtcgga gcagctatac caactttatc aggtgaacaa
     1981 atactatcaa tggttaccaa cattgataaa gttgcagatg ttgaagtttt taattttgat
     2041 gaaattccag gacctcacat aacaccttat agaatgatgg aattgaaaaa ttatgtaaat
     2101 gatttattag caagaaaaga tattacaggt gttgtaataa cacatggtac agatagttta
     2161 gaggaaacag cttattttct agaccttaca attgacaaca tcaaacctgt aatagtcaca
     2221 ggtgctatga gaagtagttc tgaacttgga tatgatggtt caagtaattt atctgcatca
     2281 gtttgtactg cagtatccaa agatgctatg ggaaaaggag tccttgtagt tttaaacaat
     2341 gaagtattat tagcttctga agctactaaa acaaatactc tttctctaaa tacatttaaa
     2401 tctctaacta gtggtccatt aggaataatt gactgcaacg aacttgtcct tacaagagat
     2461 attgtaaata gaacaattat agatactgat aaggtagaat ctaaagttgc cttatttaaa
     2521 gcatatgtag gagaaaatgc tgactttata agatttgctg ttgatagtgg ttataaaggt
     2581 atagttattg aggcaatggg tataggtaat attcctcctc aaatgctttc tggagttgaa
     2641 tatgctagag aaaaaggact tcctgtagtt atagtttcta gatgtcattc aggtagagta
     2701 ttcgatagtt atggttactt tggttctggt agagatttaa aaaatcttgg ttgtatattt
     2761 ggtggggact tgccaggtca aaaagctaga ataaaactta tcttagcact tggaaaaact
     2821 gataaccttg atgaaatcaa agatttcttt gaaaaaggaa tctattgcta attctaataa
     2881 cttctattta attatttagt tcagtttaca agaaaataca cacaaaccat aaaatgagat
     2941 gttgacaaca taacagacag tatgctcctg taaaaggtaa catactatct gttatattgt
     3001 ctggattccc aaattagcta tttggaactt aactaaaaaa tcctaatata agggcaacta
     3061 ttataatggc aatcggaata catactgtgt aatcctgttc catagagtca tcatctgaat
     3121 gtagttttac aattcgcaat ttagcaatca gtttgaagca gaaatagcct attattgttc
     3181 caattacatt cgtgagtaag tcgtctatat cggttgctct gtgtaacgta aaaagctgaa
     3241 tagtttcgat aaagagagat aatccaaaac caatcaagaa tgtatttctc aaccgttcaa
     3301 aggttttgct gataagcgga cataaaaaac ctaatggaac aaaaagtaaa atattcaaaa
     3361 taaaactcaa actaaatcca tcactaaaag gaataagatt tatgtttgga ttaaagaacg
     3421 tttcccctaa ctgtgacagt cttatatatt cactgaatgt agggatacca acaatatgcg
     3481 atagtgttaa acataagtag taatacattg ccagtgataa aatagatgtt tttacctttg
     3541 gaatatcact atttttcttg aatattacaa ctaagaaaat accaatcaag ataattccca
     3601 acaagaaaat aggattaatt aacaataaat ctttgatagc ttcaaacaga ctgagttgaa
     3661 tttctacact tgattgatta tttgatacag tctccatttg tacacacccc ctcataacat
     3721 ttcaattatt tctaaaaaac aaaatagaag ataaatctta aaatcatcct atcaaaattt
     3781 ttaaatttgc ctttatcagt ttataccacc agttaattcc tgtcttactt ctgtatggta
     3841 ttcttgggat tctttcatca aattagacaa aaaaatggtt accatatcaa taaactggtt
     3901 acgctccata tctcctgaag caaacatccc ctgagaataa atccgtatca ggaatctcca
     3961 catctactgc acctatcaca agtcatttga taacatcctc aaacaatccc aacttggata
     4021 tatcagcaac acccattaga atactactgc tgaccatcct tgttttcctg gtgataattt
     4081 taatcaactg ggaagttgaa acgcctatct tttttgcaag ttccttttta gaaacatggt
     4141 agttattata tgaatctgaa tctcgttgtc tgggtgttta agataaagca tagatacgca
     4201 ccttttttgc ctgttttcat tatataaaaa aaattttaaa aatacaataa ttatattttt
     4261 ttcgtttaag cttagagcag ttccttgatg aacaacactt tagaaactga ataatttaca
     4321 taccacttaa tacattaaca cgatttaaca ttaattataa taatatttaa aacaacaatt
     4381 agagtgtata agaatgaact ttttagttct ttaatcattt ttttgtataa aatcaaatct
     4441 atttttgtaa tttcaacaat gaaaaaaggc ttatctctat tttgagacag cctcatttat
     4501 tttataaatt tatatttttg ttcatatttt tgagttattt aacaactata tttactaatt
     4561 ttttaggtac agctacaact ttaactatag ttttaccttc aactaatcct tttatttttt
     4621 catcttctaa agctaccttt tccatctcat ctttagatat atttgagtta acagttaatt
     4681 ttcctctaac ttttccatta acttgtacaa caacttctat ttcatctttt actaaagctt
     4741 tttcgtcata tttaggccag tctatatcaa atacacttcc ttctttacct atcatagtcc
     4801 atagttcttc acctaaatga ggggcaaatg gtgcaattat agttactata gtttgtacac
     4861 cttcttttat aactgcttcg tttatattat ctaactcttt atatttatac atatcattta
     4921 ttaattccat aagagctgat atagcagtat taaatccgaa tttttcactt aaatcagcag
     4981 taactttttt aagtgtagag tgtatagtat atctcatatc cttatcttgg gaatttaatt
     5041 ctccaaactc tacatccttt ttaaccacat ctgctaactc gtcaaccaat ctataaaccc
     5101 tatttaagaa tctaaagcat ccatcaacac cttgctctga ccagtctaaa tctctttctg
     5161 gtggtgctgc aaataatacg aaaagtcttg cagtatctgc tccatactca tctattatat
     5221 ccataggaga tactgtattc cctttagact tactcatttt agaaccatcc ataagtacca
     5281 ttccttgagt aagtaagttt ttaaatggct cattgaagtc taccattccc atactcttaa
     5341 atgcctttac aaaccatctt gcataaagca aatgcattat agcatgttct actccaccta
     5401 tatattggtc tactggatgc catctattaa ctaattcctt actaaatggt tcattttcat
     5461 tattgctatc cacatatctt aagaaatacc aagaagaatc aacgaatgta tccatagtat
     5521 caacttccct tctagctggt tttccacagt gaggacaagt tgttgacata aattgctttg
     5581 aagtcgtaag tggagattct ccctttccag taaattcaac atctgttggt aaaagaactg
     5641 gtaaatcttc ctttttaact ggaactattc cacattcatc acaataaacc acaggtatag
     5701 gacaacccca gtatctttgt cttgaaacta accagtctct aagtctataa ttaattgttc
     5761 tctttcctct accttctttt tctaacttat cgattattcc ttcaaatcct tctgaagcat
     5821 ctaatccatt aaattcttct gaatttatca ttttgttatc ttctgttatt acatcaatta
     5881 tgtctaaatt atatttttca gcaaaatctc tatctctttc atcatgtgct ggaactgcca
     5941 ttatagcacc cgttccatac tcaactaata catagttagc tatccataga ggaactttct
     6001 tgccatttaa aggatttata acatatctac ctataaacat tccttctttt tctacatcag
     6061 ctgctgttct ttctatttct gtcatagtat gcattttttg aacaaaagct tcaacatctg
     6121 cttcatattc agtccctgca accaattctt taactaattc atgctctgga gcaagtacca
     6181 tataagtaac tccataagtt gtgtctggtc tagttgtaaa tactgtcata gatttatcag
     6241 ttccatctat atcaaaaact aaatctgctc ctgtactttt acctatccaa tttttttgca
     6301 ttgttttaac tttttctggc catccatcta atgtatctaa gtcattaagt aattcttctg
     6361 caaattcagt agtcttaaaa taccattgtt ctaaatcttt ctttaatact gttgctttac
     6421 atctctcaca tgctccttga acaacttgct catttgcaag aacagtctca caagaaggac
     6481 accagttaac atatgatttt ttcttatatg caagcccatg ctctaaaaac ttcaaaaata
     6541 tttcttgtgt aaatctatag tattctggag ttgaagttgc aacttcttta tcccaatcat
     6601 aactaatacc taataaattt aattgctctt tcatttcttc tatattttcc attgtccatt
     6661 tatgcggatg tattccatgt tttatagctg cattttcagc tggtaatcca aaagaatccc
     6721 atcccattgg atgaagtaca ttatatcctt ccattttttt gaatcttgcg acaacatcac
     6781 ctatagaata atttctaaca tgtcccatgt gtatttttcc agatggataa gggaacattt
     6841 ctaatgtgta ataattaggt ttttcagttt gtgcagtatc cattttatac tgattattat
     6901 ctttccaaat tttttgccat ttcgattcta cttctttaaa attatatacg ctcatatttt
     6961 gcctcctatt tttaattgtg tataactacc ttatttagta ttatttactt ataatttcca
     7021 aataattctt tgaaaataaa aaatcctcgt cactaaactt acgtttaggg acgaggataa
     7081 atcttcgcgg taccacccta attagcctac tacatatata gactctctta gatagctttt
     7141 aatccagcca tggagaatag attttaatct agtaagttca aagttatata atattagttc
     7201 tcaccttcca ctaactctct gaaaaaatat aatcttttac tgctctagtc tattcaattt
     7261 aatataattt ttgaaatttt ctaattataa taacatatat tatcatgttt atgctaatgt
     7321 gtcaatatct atataaggtg catctttcca taatttttct aaattataaa accctctatt
     7381 ttcctcatta aatacatgaa ccattatgtc tccataatca aggagtaccc aagtttgagt
     7441 tccttgtcct tctttacctc ttggctctaa tcctagtttt gctaattcat cttccacatt
     7501 gtcagcaata gcttttacct gtctctgaga agatgctgta gtaataataa aataatcaca
     7561 caaactagaa acttttccta tatttattat aactgtatct tgacccagtt tatcttctat
     7621 ggcatcatat gctatttttg tcatttgttc cactgtcata taaaaattcc tcactttcct
     7681 aattaaaata cacttagtta ttgtaacata ttaatacgat tttgtaacat agtcaaattt
     7741 aaatttatta atatgtttgc ctttatatag cttttttata ataacttttc tttaattaga
     7801 tagtttcttg ccataactgt tcttggatgt atctcttctt ttttattgat gacaaacatt
     7861 aaagtattat taaaagaagt taaaagtgct tcatcaagct tccctccata tgctaattct
     7921 cttaatgtat ctacaactgg aaattttctt ccttcttcaa ttaaatcagc tatgtaaatt
     7981 attttttcta aaagagacat gtcctctctc cctgttgtat gatatttgat tgcatttata
     8041 atatcttcat cttctacttc aaattcatac tcgcatatgt acgccccaat tacactatga
     8101 gacaaggaac gatttccatc ctctattggg tctaaatata tttcatactt atttacataa
     8161 tcctctattt ccttgtcact taaatacttt gcacaatcat gaatcattcc tgctaagtaa
     8221 gctttttctt tgtcacatcc ataaatctca cataattttt cagcacattt tgcaacattt
     8281 cttgaatggc taagcctccc cactggtagc atttgattta gtctttggtt aatattttct
     8341 aagttcattt aatcatctcc atattgatat agtttatttt catataaata attttctaca
     8401 ttttcgggta ctaagtaacg tattgacttg ccttcattta gctgttccct tatatatgtg
     8461 gacgatatat ccagtgatgg aacataaact gttataatgt cagcattata cttcttagtt
     8521 agtttttcta tagtctcttg tgaccttaac aaactaattc ctggtctagt tgcggctata
     8581 aaagtagcta actcaaagtt tttctttaca tctttccact cttcaacact acaaattgca
     8641 tctgcacctg ttataaaata aatgtctgca tttttgtatt ttttctttaa gtattttaat
     8701 gtatctattg tataagtcct tttctttctt tcgatttcta cttttgaaac taaaaaatct
     8761 tcgttgcttt cagttgctag aagtgtcata ttatatctat catacttatc tgtagtgata
     8821 cataatttat gtggtggatt tcctgatggt ataaagatta ttttgtcaat gtcatacttg
     8881 tctctaataa attctgctgt tgctaaatga gcatagtgta tagggtcaaa agtacctccc
     8941 aatataccta ttttgatttt gcctttatgg cgattaaatt tctctagttt ttcagtattt
     9001 ttcaactctg ccatcttaac tagattttca cttctcatat tttgtctcct tttaatgtta
     9061 taatactatt gtaccattat tttttatttc ttaaagctta taatttgttt ttttgggtat
     9121 ataattatat ttatatgcta aaaaacactt taaaaaataa
//
LOCUS       DAMJVX010000136         9139 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMJVX010000136 DAMJVX010000000
VERSION     DAMJVX010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9139
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.141"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            324..980
                     /locus_tag="PC407_002812"
     CDS             324..980
                     /locus_tag="PC407_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498239.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(1450..2721)
                     /gene="sleC"
                     /locus_tag="PC407_002813"
     CDS             complement(1450..2721)
                     /gene="sleC"
                     /locus_tag="PC407_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HDF2498240.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIVAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGADTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            2966..3682
                     /locus_tag="PC407_002814"
     CDS             2966..3682
                     /locus_tag="PC407_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDF2498241.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            3761..5071
                     /locus_tag="PC407_002815"
     CDS             3761..5071
                     /locus_tag="PC407_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDF2498242.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            5171..5707
                     /gene="lepB"
                     /locus_tag="PC407_002816"
     CDS             5171..5707
                     /gene="lepB"
                     /locus_tag="PC407_002816"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2498243.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            5873..6409
                     /gene="lepB"
                     /locus_tag="PC407_002817"
     CDS             5873..6409
                     /gene="lepB"
                     /locus_tag="PC407_002817"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2498244.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            6419..7174
                     /locus_tag="PC407_002818"
     CDS             6419..7174
                     /locus_tag="PC407_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDF2498245.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDIRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVREG"
     gene            7248..8915
                     /locus_tag="PC407_002819"
     CDS             7248..8915
                     /locus_tag="PC407_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HDF2498246.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVILIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
BASE COUNT         3428 a          945 c         1708 g         3058 t
ORIGIN      
        1 agaatatcag aaaaaataga taattaaagg gttagggtga aagagtaatc gtaaaataaa
       61 tatatggatg atggtagata agaatatcag aaaaaaatag ataattaaag ggttagggtg
      121 aaagagtaat cgtaaaataa atatatagat aatggtggat agagtagtaa gaaaataaat
      181 agataaaggg atagaataaa tttaaatatg gaaaatgaat atctaataat aagtgattaa
      241 taaataattg tagatagatt gtaaattaaa taaatcttta atagaaagga gaactattta
      301 tatattaaat agtatttata aatatgaaaa aaggcgattt tatatggagt ggagttctta
      361 tttgtattat tgctgtctta gcaataccaa gtagtagaga agtatttatg gaagcaacag
      421 gtgcacatcc atatattggg ggttttttaa agtttgctgt attagctaca atgggagatt
      481 tacttggtgc tagaattttg tataaggatt ggaaaatacc agtaggagta ttttatagag
      541 caatagtttg gggaataata ggtgtagtta ccacatatgg aatgtcaatc tactcatcag
      601 gtgtaggtca agcacaagtg gatggtatgc ttccatttga gggaatagct tttgcaaatg
      661 ctctatttaa gagtgcaact atgaatatac taatgtcacc aactgtattt ttattccata
      721 aggttatgga ctgttttata gataaaaaat atgaggttgg aaaggggatt aaggtaaaac
      781 ttaaagatgt ggtaaatatt gtggactgga atgcttacct tggttttagt gttttaaaaa
      841 ctataccatt tttctggata ccatgtcata caattgtatt cttattccct gctgagtata
      901 gagtaattgc ttctgcattt gcatctatag ctttaggtct aatattggct ttagctaata
      961 agtctaaagt tgcagtttag gttcactgat gaagataata gtattattta aattatgaat
     1021 aactttttaa attaatatta tttatattta taataaataa taatttagat aagaaggaat
     1081 attctaagtg atagatagtg ttttaaataa taaggagtaa tattctaagt gagaagtagc
     1141 attttaaata ataagtgaga ttttaaataa aaagtaagct tttcgttaga atagcatttt
     1201 attaaactta aaaataaata taatatttta aagaaatcta agttgctaag cactgttttt
     1261 aagaagctta aaatccccca tttaaagttt taagattcta aaaaaacaag ttataactaa
     1321 aaaatggctg ttaagactac aatacttata agtaaaaata cttatggtat atattagttc
     1381 ataacagcca tttttatttt aaattaataa ttttattcaa taatttgagt ttatgttcta
     1441 aatcaagctt taaattaaag gatttaaaga agctattcta gttgtagcta cataaactct
     1501 ggatatttca taccatgttt taaaatctac tactccgctt tgaggtaaac caaagattcc
     1561 ttggaatatc ttaacagcgt tttctgtatc tgctccatag ataccatctt ctataacttt
     1621 tggtacagct ggataagaat tagatatagc atttaactga ttttgtatgg ttctaacata
     1681 ctgcccagaa gaaccaactt gtagtgtagt accagggaat gacactggta caccacttac
     1741 tataggtgct ctttcaaata ctatctcatc tccatagaaa tatcttagga tactctcata
     1801 gtcatatcct tggtcaccta agtctttact gccccattga gtcatttggt caggacattg
     1861 agatttttgt ccatcacaat attgtgctaa tagtggctgt cttgatgtag gaggtctttt
     1921 tataaaagta ttaaaaatag catctacaac tacgttaatt ggttcaaata agtttctatt
     1981 atttataaac ttatggtcgt atgctgttgt tgaagttatt gtaaagttat atcctttatt
     2041 tctataccat tcagtaaaga ctctatttaa agtaaatgat attatagcta ttacatttgc
     2101 atagattgtc tgttcaggcc aagttgaata tatttcagat gacgctatgt ttttaatgta
     2161 ctctttaaat ggaatccaat agtttggagc tgatgaatct tctggcaaac catcatgaac
     2221 tactataaat tcaggcacta cagggttatc taatacaacg aaaccagtag gaggtggtaa
     2281 tggttttaag ttactttctg gaatcttagg aggataagta ccatatagag tatgttctcc
     2341 tatgtcaaaa atcaactcac tttgtcttga gaaagaacgc ttacttcttg ttctaggaga
     2401 catgggaact ccttgtcttg ctacaattgt agctaacaac tgcgttcctt ctatgacaac
     2461 tgtctcatat ccatctgcta tggcttccac tatatactgg ctataaggtc taacatcaga
     2521 tggctgtaga gagtaatcta catctggtgc tggtaatact aaaccagtca cttgaccaga
     2581 ttcattactt cttaggtttt ggtataaagt tgatgagctt tggctaccat tagacattga
     2641 atatatgttt actactgcat tttgaatagg tctattgtta gtagcatcaa ttatgcttac
     2701 tgttaagaaa ccatcttgca tcaaatcacc ctttctttaa atgaatttag ttcccttata
     2761 tttcaagata tgaatatagc ttgaaatttg ttaaataata tagatataaa tttaaatttt
     2821 tttgagtatt tgagtatatt tgtatttaaa taatttaaaa taatttaatg tatattttag
     2881 tatgtattta aaacacaaat aagctaaaaa taagtagatg atataaatca taaataaatc
     2941 aaaaattttg aaaggaaaat aatctatgaa gctagattta tttttattag caatagttcc
     3001 tatattgata gggatgtttt ggataaggtc taaagataga tattgtagag aacccctaat
     3061 ccatctaatt aaattttttt taataggtgc ttttctaagt gttataatta tacttttaga
     3121 aaatttactg atgaagttta atgtatttga agggtattca gaactaatat atgtatcatt
     3181 tgtagttgca ggacttgtgg aagaaggtgt aaaagctctg atactgattc ctgccctaat
     3241 taaagaaaaa cactttactg aaaagttaga tggtattatt tactctgtat ttttagcttt
     3301 aggatttgca acaattgaaa atatggttta tattttttct gaaagtagaa atctagcact
     3361 gcaagttggt attaataggg cagttatctc tataccagct catgtaatgt ttgcaattac
     3421 tatgggctac tatatttcta agtataaatt tgagggaaat aaaaataaaa gacgtgagta
     3481 tttgtttatg gcagtgttaa ttccaatttt attacatgga gtttttgatt tcatattgat
     3541 gatagaatat agatgggcta ttatattact tatagtttat gtaataattt tgtggaagat
     3601 aaatcttgat aaacttgaga aatatatgaa tcactctaaa aaagtgtttt tcggtaatct
     3661 taggaaaaag aagaagaaat aaaatttaaa ggaaagacat aaataattaa agtataataa
     3721 atgacacaca agtaaagcag attgaagggg gattatagat ttgacagaat taccaaagga
     3781 atttttagaa gatattgaag aaatactaaa agatgaatat gatgatttta taaaaagtta
     3841 tgaggaaagt aagactactg ggcttagaat aaatacttta aaaattgaca agaaaaaatt
     3901 atttgatttg gagttgtttg atttgaatca aataccatgg gcagaagaag gtttttatta
     3961 tgatgataaa attgacagac ctggtagaaa tccactgcat gaggctggag catactattt
     4021 gcaagagcct tctgcaatga gtgtaatacc aaaggcagat attaaagagg gtgaaaaagt
     4081 tctcgatatg tgtgctgctc ctggtggaaa atctacatat atactatcta agctaaacga
     4141 tacaggttta ttggtttcta atgaaattaa tgctagtaga attaaggctc taggggaaaa
     4201 tttagagcga tttggagcga gaaattgtat tataacaaac accgattcta atagcttaag
     4261 aaagacgttt aatgggtatt ttgataaggt tattatagat gcaccttgtt ctgggcaagg
     4321 tatgtttagg aaggatgagg ttgccatatc agattggagt tatgcaaagg tagtagaatg
     4381 tcaatccatc caaaaagaga ttatcagaga tggatataaa atgttaaaga aggatggcat
     4441 tttaatttac tcaacttgta ctttttcaag ggaggaaaat gagcatgtta taaatgagtt
     4501 tatcagtgaa tatccacatg cagaacttat tgaaatggaa agattatggc ctcataagat
     4561 aaagggagaa ggtcattttg ttgccaaaat aaaaaaatta gaagaagaag aatgtaatgt
     4621 caaagaatta aaaataaaaa atttagataa agaaataaag gattataggg agtttgaaaa
     4681 gaaattttta aaggttgatt tttatagtaa taaaaaaaat aaattttatt taagaggaga
     4741 aaatttgtac ctacttcctg aaatatatcc agatacgaaa aaactaaaag tattgagata
     4801 cggtttgcat ttaggggttt taaagaagaa tagatttgag ccttctcatg cactatctca
     4861 ttacttaaaa gttgaagatg taaaaaatgt tgaaaacttc agtgtacaga gtgaatcaat
     4921 tcttaaatat ttaaaagggg atgttgtgaa ttctaatgat agtagaggat gggttttggt
     4981 ttcggttgaa ggaatacctt taggttgggg aaaagaatct tctggtgtaa ttaagaatca
     5041 ttatcctaaa ggtcttagaa aagtatttta aactattaga aaaaagaagt ataatattta
     5101 tattgtaggt ttatggtaga ttattttgct gtagattgac ttaatattta acacagagga
     5161 gacgatttta gtgggtgaag cagttaaaaa agaagttgta gaatggataa aagtgattgt
     5221 catagctctt gttttggcat ttgcaataac tcgttttata gtgccaacaa tagtcaaagg
     5281 agaatcaatg tatcctacat tagttgaacg tgattatttg atagttaaca gaattgcgta
     5341 caaggtagga gagccaaaat acaaagatat aatagtattt aaaaccgact taacagagga
     5401 aaatggaaag aaaaaagatt tagtaaaaag agttatcggg gttcctggtg accatgtaaa
     5461 aatacaagac tccaaggtat atgtaaatga taagttgtta gatgagactt cctatataca
     5521 taataatcgt actgatggag atattgatat cgtagttcca gaaggaaaat tatttgcaat
     5581 gggagataat agagaaaaaa gtttagatag tagatacgat gaggttggat tggtcgacga
     5641 gcataccatt ttaggaaagg ttctagtcag attgtatcca ttttctaaga taggaactat
     5701 tgactaaaca tataaaattt ggagcaatcg ctgattagct gaaatcacca gaccatacac
     5761 aatagttgtc tatataaaaa agacaacacc ttatttagta gacttatcta aaagatagtg
     5821 tttaagagac attatcaata aagataatat aactataaag gagaatttgt gaatgaatga
     5881 aactattaaa gaagagattg tagagtggat aaaaataatt attactgcac ttttttttgc
     5941 atttattata actcgtttta taaaaccaac actagtaaat ggagaatcaa tgtacccgac
     6001 acttaaatca catgattatt tggtagcaaa taggatgaca tataagttat cagaaccaaa
     6061 atgtggagat ataatgatat ttaagactga tttattacaa gagaatggaa ggaaaaaaga
     6121 gcttgtaaaa agggttatag gtgttcctgg tgaccatcta aaaattaagg attctaaggt
     6181 ttatataaat ggtaagttat taaatgaagt ttcatatata catgataatt atactgaagg
     6241 cgatattgat atggtgattc ctaagggaaa agtatttgcg atgggagaca atagagaagt
     6301 tagtttagac agtagatata aagaagtggg attggtagat gaagaaaata ttaaaggaaa
     6361 agttatttta agagtatttc cttttacaga tataggtatt tttgagtagg tgatttgtat
     6421 ggcagttggt atagcgtcct tattgtttaa tattgaggag gctttaaaca tttgtgagag
     6481 tataaagcaa ataactcatt tagagattgg aattgacaat atatctgaat gtagcgagtt
     6541 atgtaaatat aaagagagaa tttctaagtt aggattatct atagggatac acttgcctat
     6601 ggaattaaat acttgcgaaa acatagagta tatacgaaat tcatggataa attttattga
     6661 gaagatagaa tttgaactaa aaggatttga tataaggtat tttaaccttc atttgggata
     6721 tgttatgaca aatagagtta gaaagaatag agacaaatat ttaggaaata gtgtggattt
     6781 tttagataaa ttaaatacaa attcatatgt atgtatagag aatacttatt caaaaggtgg
     6841 ggatttttca aatattggaa atatatctta tgattttgaa tatatattta agaggattaa
     6901 aaattctaag atttgttttt gttacgatac tggacactgt ttaattgatg aggatgctta
     6961 tgtaaagaac ttaaaggata aaataagact gatacactta agtgataatg atggtatcaa
     7021 tgatacacat gttggtatag gaagaggaat tttatctgaa gaggggatta aagaggtctt
     7081 aacattagat gcagaatatt tagtattaga aataaattac gaagacattg aagatactat
     7141 aagtaaattg aataatattg taagagaggg ttaaatacct ctctttatct tgttgatatg
     7201 cctcattaac taaaatttat attcatcaaa aggaaggaga tgttgaagtg aaaaaaataa
     7261 acttaaaaat agaaggaaaa gataaggaat attcattaga agaaaattcc cctggaatca
     7321 gattgggaga tatagcgaaa gaattttgtg atgagcataa gggttatata acacttgcag
     7381 ttgtagacaa taaactaaaa gagctaaatt gtagagttaa gaaggattgt gagataaatt
     7441 ttttagacac tactaatgaa gatggagaga gagtttactt tagagtcatg tcatttatat
     7501 ttgtaatggc ttgtagagaa atcttttggg atagcagagt tacaatagaa cattcactat
     7561 cagatgggct atattgtgaa gttcatatag atagaaagct taaagaagca gatgttgaaa
     7621 atataaaaaa caagatgaaa gagatagtaa acaacgatta tgttatagaa aaaattgaag
     7681 ttacaagaaa tgaagctatt tctatatttg aaaataatga aatgtatgaa aaggcagagc
     7741 ttttgaaata caaagagtat gatagtgcta aagtatataa gtgtagaaac tatatagacc
     7801 atttttatgg ttatatgttg ccatcaactg gttatataaa atcatttgat attaagcttt
     7861 ataatggagg cgttataatt ttagggccta gtgaagaaga taagactctt cctatgaagt
     7921 ttgtacctca acctaagcta tcaagtgtat attatgaagc agaagaatgg tcaagactta
     7981 tgggaattga caaggtagta tctcttaaca aaataataga aaacaatgaa tatggagata
     8041 taataaggac attcgaagca ttgcatgaaa agaagttatc ccaaatagcg gatatgataa
     8101 aggatggcaa taagagagta attttaatag cagctccatc ttcatctgga aaaacaagtt
     8161 ttgctcacag attatcaata catcttagag taaataattt aaatcctatt tcaatttctt
     8221 tagatgatta ttttatagat agaaaacata ctcctttaga tgaatatgga aattatgatt
     8281 ttgaatcaat ttatgcaata gatttagaga agtttaactt agacttaaag aaacttttag
     8341 ctggtgaaga aattgatgat ataagattca attttaaaga aggtaagaga gaatacactg
     8401 gtaaaaaaat aaagatagat tcaaatcagc caattatatt agaaggtata catgggctaa
     8461 atcctatttt aacatcatct ataccagatg aagataaatt taagatatac ataagtgccc
     8521 ttactcaaat aaatttggat gaccataatc gaatccctac tgctgatttg agaatgataa
     8581 gaagaattgt tagagactat aattttagag gatatagtgc tgataatact attttacaat
     8641 gggcatcagt tagaagagga gaaaagaaga atatattccc gtaccaagag gaggcagatg
     8701 ctatatttaa ctctgcttgt gtatatgaat tagcagtttt aaaacgattt gcgaaacctt
     8761 tgttggaaga gataaaggaa gataatcctg cacatattga agctactaga ttactaaagt
     8821 ttttgcagta ttttgttgaa ttggacgata cttctgacat accaggtatt tcaattctta
     8881 gggaatttat tggaggaagt aaaatagttg attaatatgt gtattataga ttgttttatg
     8941 aagattaaat ggcatagcaa gtagtgtgat tggtttatgt taagtatatt aagtgtaaag
     9001 tggagaatta aattgataca gattttaaag tttgagttga tatataaaaa ggcttgtttt
     9061 atacttttgt atgaagcaag ctttttattt taaattttac ttttttgttt aatattttgt
     9121 tagtttatgt gataatttt
//
LOCUS       DAMJVX010000137         8935 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMJVX010000137 DAMJVX010000000
VERSION     DAMJVX010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8935
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.142"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(<1..2019)
                     /gene="recJ"
                     /locus_tag="PC407_002820"
     CDS             complement(<1..2019)
                     /gene="recJ"
                     /locus_tag="PC407_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDF2498247.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRY"
     gene            complement(2032..3696)
                     /locus_tag="PC407_002821"
     CDS             complement(2032..3696)
                     /locus_tag="PC407_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDF2498248.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            complement(3994..4491)
                     /locus_tag="PC407_002822"
     CDS             complement(3994..4491)
                     /locus_tag="PC407_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDF2498249.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            complement(4520..5899)
                     /gene="scfB"
                     /locus_tag="PC407_002823"
     CDS             complement(4520..5899)
                     /gene="scfB"
                     /locus_tag="PC407_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HDF2498250.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            complement(6204..6350)
                     /locus_tag="PC407_002824"
     CDS             complement(6204..6350)
                     /locus_tag="PC407_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF2498251.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            complement(6380..6958)
                     /locus_tag="PC407_002825"
     CDS             complement(6380..6958)
                     /locus_tag="PC407_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF2498252.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKFVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            complement(7326..8756)
                     /locus_tag="PC407_002826"
     CDS             complement(7326..8756)
                     /locus_tag="PC407_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2498253.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKALKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEDQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEEICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
BASE COUNT         2841 a         1602 c          829 g         3663 t
ORIGIN      
        1 atatcttttt aagtctattt tatcaatatt aggagaaaat ataagctcta atttatcagt
       61 atttttgcta acatcttcaa taatattgta atttatgtta aaatccaaat ctataagtga
      121 tatatctgac attgctctat aaaatccatt tatagaatta gatattatta gtgtatcttt
      181 ttcaaaataa tcaaatatat ttatatctgt atttccatca aataattcaa aatcagacac
      241 agatatatta tacaggcttt gagtatcttt tggtattatt tttgacatta atttcaatga
      301 aagtttgtca ttacttgcac ttttaggtcg agccaatcgt atatctttaa gtaaaaattg
      361 tactttccta tttcccatat agttattttc atctaattgg aatagtacat ctactttgtc
      421 tcctacttta tacattgatt ttaaatgact catattaaat cctatacatt catagctttt
      481 ctctttttct atacttaatt tcagatgttg ttgattctta cctataactt tcaaatcttt
      541 taaaatataa tttctaacta taaatctagg attaggatta tttaatccaa agggttctaa
      601 cttatgtaat tcttctacaa gatttaaatt tattacattt tcttgtaatt caaactcgac
      661 atttacattc tctatcatat catcttcagt taaattataa tctgcaaatt tatttatttc
      721 atttgctaaa atttctacat tgtctgaatc caaggataac cctgctgctt gttcatgacc
      781 accaaactta gtcatcaagt ctttgcactt tatgagagct tcaaatatat taaatccttt
      841 tattgacctc gctgaaccag tagcctcccc attttctata cctagaagaa ttgttggttt
      901 gtagtatttt tcagttaact ttgatgctac aattcctatt attccatgtt gccaaccctc
      961 tttggctaaa accaacactt tgtcatcatt gtatctgcta ttagacttta gcatttcttc
     1021 agcttcatga tacatcttag cttctatcat ctgtctttca ttattttttt cttccaatat
     1081 actagctatt tctactgcct cttcttgtga ctgagtagta aatagctcta ctcctaagta
     1141 agaatatccc aatcgacctg aagcatttat cctaggacct attgcaaaac ctatatgaga
     1201 agaacctatc ttatcacttt ctacaccaca aacttttata agctccctta atccaatatt
     1261 tttgccttct ttcattgatt ttaaaccatt ttttacaatt attctatttt catctataag
     1321 tggaacaata tcacaaatag ttgctagtgt tactatttcc aaataattat acatactagt
     1381 tttaaattct tcttttggag tcaaggcttg aatcatttta aatgctacac cacatccaca
     1441 tagtgaatca aatggataat tacaatcttc ttgttttgga tttattactg caaaggctga
     1501 tggaatttca ctttgacatt catgatggtc tgtaattata acatctatgc ccaaatcatt
     1561 cgctgtattg acctcagaca ctgatgtaat tccacagtca acacttataa tcaagtcaca
     1621 tcccctagag cttataagct taatagcatc ctcatttata ccataccctt cttctaatct
     1681 attaggaata tagtaatcaa cattgtaacc tatactctta aaatataaac acaagatgga
     1741 tgtagatgat acaccatcaa catcataatc tccgtaaatc caaatttttt cgttcttctc
     1801 aactgctaac tttattctat caacagcttt ttgcatatct ttcattaaaa aagggtctct
     1861 taaataatct aaagatggat tcataaatat ttctgaatct ttttcagtac atatccctct
     1921 attttttaaa atttggcaga tttccgggga aatattaagt tttttgctaa attcactttc
     1981 ctctatctta cctttatact tcaatatcca ttttttcaaa tttaattccc cctaatcttt
     2041 tttgtatttt gacctaatac ttcttataac taagcaaagg cctattataa aggatataaa
     2101 gaaacccgct attgcagtaa attttatcca tatattattt tctatatttg gagatgcaat
     2161 tataagagaa gaacctacta taatagatgc caacactaaa ctcaaagata tttgagatgt
     2221 tagctctatt atacaatttt ctaacctcgt cattttaaca tcttcaattt gcatttttat
     2281 attatttctt tctatatttt ttaaaattgc ttttaattgc tttggaatag tttttatgtc
     2341 cagtaatatt tcttctacat tttgtcttga acttaaaaca acatttttag ggttaaattt
     2401 actcttataa tgatgtttca taaactcttt tcccatagaa ccaaaagaaa aatctgtgtt
     2461 taaagttcta cttgttcctt ctaatgttat aactgtcttt gcaagagtta caaattgtgc
     2521 aggcatgact attttatact gtctaaaaaa cctaaatagt tcatttaata tatctgttac
     2581 atttattttt tctatagaaa tatcatagta ataatgtatt agataaagta aatcctgtct
     2641 aagtccctta atatcagcct ctccatttaa tgcatccatt tctatcaata gatatattat
     2701 cttgtcaaca ttcttttcta cacctgctaa tgcaatttca tttaattggt ttaaagtttt
     2761 attatcaata attccaatca ttccaaaatc tatatatgct atcttatttt tagcaactac
     2821 aaatatatta cctggatgtg ggtctgcatg aaaaaatcca tgagataata cctgtgtaaa
     2881 aaatgaccta actcctattt caacaatagt ttttgtgtta taacctagtc tccttatttt
     2941 ttctacatca cttaactttg taccattaac tttctccatt actaaaattt tcttagtatt
     3001 atattctgag tatactttag gtatatatac ttcatcacta tttttaaata tcctagagaa
     3061 tttagttgca tttatggctt caaatgtata atcaagttcc ctcattaatt gagtttgaaa
     3121 ttcttcaatc atctgtacta aatctaaatt aaaatccttt tttaaatctt ttaaagtatt
     3181 tgcaatagtt ctaagaatct ctaaatcaga ttttattatt ttctctatat ttggtctttg
     3241 aatttttacg attacttctt ctcccgtttt aagtacacct tcataaactt gaccaattga
     3301 agctgctgct attggttctt ctttaaattc taagaatatt tcttctatac ttaaacccac
     3361 ttcttcttta aatatatcaa tagctatatt tgaatcaaat ttctctacat catctctgag
     3421 ttttgatatt tcatcaatta tatcctggtc taacaagtct tttcttgtac tcaatatctg
     3481 tcctattttt atatatgtag gtcctagttc ttctaaaact cttttcattc tctcaccagt
     3541 tgtcatattt tttatttctt ctggtgggtc aaatagtggt attttatagg caattccttc
     3601 tatatttagt ctttctacaa taaaactaaa accatacttt attaaaacat atccaatttc
     3661 tctatatcgc tttaaatttc tataacttat tctcaaatct tttcactcct aagataggta
     3721 tacccaaaaa cttacacctt aatttatcgt atatgacatt atttcaccaa ttgatattct
     3781 aatatcctat atcctcttta atatttaatt ttatcacata tactaccaat tttattaaaa
     3841 ttatttaact cagttaattt ttatttttga cattttatat ctggtttaat caaatacttt
     3901 ttgaaaacta aaatatcaac aaaatctaat aaaataaatt agtggttgcc tcaaaacaga
     3961 tttaaatatg ttttgaaaca accactcttt aaactaatat ttttgatttt gtgctgcctc
     4021 taaataccat tcataagatt catcaatata ttttttatct tcttctgtaa gctctctaac
     4081 aaccttacca ggagaaccca ttatcaaaac acctggtgga aacttcttat tagatgtaat
     4141 tagtgtacct gctcctatca gagtatattc tcctatttca gcattatcca atattattga
     4201 acccatacca attaacacat tatcactaat tttacaacca tgaactatag acctatgccc
     4261 aactgttaca ttgttaccaa ttatagtttc tgtatctcca tgcactatag aacaatcttg
     4321 tatattagtg ttttctccaa tagttattgg tccttcatca cccctaacta ctgcattgta
     4381 ccatatactt gaatctttgc ctattttaac atttccaatt acatctgctg attttgccac
     4441 aaatacactt tcgtcaatta aaggtttatc ttctaaataa tctctaatca tatataaaca
     4501 actcctttaa aatataaatt tagttggttg ctccttctaa cattaactta gcttgtatca
     4561 ttatagagca ctctgtacgt tttctttgca tttcacatcc taattcatat ggttttgata
     4621 tgtcattatt aaagtttatc gcatttgcat gacatccacc tgaacagtaa aacttattcc
     4681 aacattgttt acactcttct ttagtatata catgagcttc tctaaacata ttttttaaat
     4741 tttctggaag tactatttct tcatcaaata tatttgccat tttaaattct tcatttccaa
     4801 caaattgatg acatggatag atatctccat ttggagttac agaaagatac tcatttccag
     4861 ctccacatcc agttattctc tttattacac atggaccttg atttaggtct atcataaaat
     4921 ggaaaaattt aaatccatct ccttgtaatt gtctatctgc atactctaca gcaaattttt
     4981 cgtactcttc aaatatctta ggtaaatctt cttctctaag tgcatatgga ttactctcat
     5041 ctccaacgac tggctctaca gaggttaatt taaatcctaa atctgcaaaa tgcataacat
     5101 cttttgaaaa atctaaatta tctcttgtaa aagtacctct tatataataa tacttatctt
     5161 ttgctctttt ttccacaagt ttcttaaatc gtggtaatgt aatatcataa cttcctttat
     5221 catttaaagt tggtctcata ttatcattaa cttcttttct tccatctaaa cttaaaacta
     5281 cattatgcat attttcattt atataatcta taatctcatc atttaacagt acaccatttg
     5341 tagttattgt aaatcttata tttttattat aatctttttc tacacttctt ccatagtcaa
     5401 ccagttgttt tacaacttca aagttcatca aaggctctcc accaaagaaa tctatctcta
     5461 ggttctttct acttcctgaa ttagcaatca aatagtcaat tgctgcctta ccaacttcaa
     5521 aactcataag ttctttttct cctccaaaat caccttgagc tgcaaaacag tatttacatt
     5581 ttaagttaca atcatgagct acatttaaac ataaagcttt tactacaggc tctctatgta
     5641 caaagcttgg atgatattga tatgtatctt ctgtgtataa aagaccttct ttttccaaat
     5701 ttaatatttc ttcatatgct tcatttattt tttcttcttg atattttgat tttaatatct
     5761 ctaatatttc ttctttactt ttttctttat aaaaatctaa caaatcgtat gcaacatcat
     5821 ctaacacatg aacagctcca ccattcacat ctaaaacgat attatatcca ttcatagaaa
     5881 atttatgtat catactcata ccctacctcc taaattacta tctatactaa agttttttat
     5941 tattttaatt aaactttacc cttcattata tcataagttt acataaaata catacaatac
     6001 aaaaatgact tactctccgt aagtcatttc attaaataca ctatatcccc ctaaattggc
     6061 gcactaatat attaccaaat aggtattagg ctcctataaa ataaataaaa taaattttgt
     6121 atctgatttt atattgtttt aatcttatta aaatataata atttcttttt tcctgataat
     6181 tttgtataaa taagtgcttg tatttaattt gtcttcttta gattgctgat ttctttgggt
     6241 actttaggct gatgaataat ccaaggacag gtactattag ctgacaaaat agcagtgcta
     6301 agtgccaatg agctagcaaa cttcataaat cttacaataa actttttcat aacttaactc
     6361 tccctcatac atattttact tatactttag cttttaactt acctaatact aacatcacaa
     6421 aaatccaaaa catggcaaat gcgctatatg tgaaataatc tacaaatata ctcaaagaaa
     6481 gacttaaaat tgttatcaat aacactactg tagatataaa tatagcagtt ttcttataaa
     6541 caaacttttc cctatcactt atagggttac tcctatgttc tattggacac agaacacaaa
     6601 ttccaaccca actaacactt gcaataatca taataatatt tttaaataag tctatattta
     6661 tattttctat aattaagatt gtagataaaa ttataaaaat aaaggttaat aagcatctaa
     6721 aataattgtc tgcatgatac cctcctgaaa attgtcttat tggacaataa cacattaaaa
     6781 aaaataatac atatgtaaat ttaccaaata aaaaacctat aaacagcatg gtggttatat
     6841 taactatgaa agctattaat atttcaaatc cataagaata gactttagat tcattattat
     6901 ctatcatatt attacaaatt aaaacagatg tcattttttc agcatatctt ttaaacattt
     6961 ttacctccaa aaaaatttta catatataga gtacattctt ttttgagaaa attcaatata
     7021 ctttacaaaa ctagttgtta aaaatgcaaa actggtcaaa aaaagaccta tataattttc
     7081 ttattattat ttatataaca aaacaattat aataaaacaa ttttatcaag ctttatatgc
     7141 ataaaaagcc tatgtcaaaa tagaaataaa ccttttcccc attgttaaaa ggctaaaaat
     7201 atattttatt tcatataaaa agagtacctc acaaactaaa aagttcattt tgagataccc
     7261 attttattta ttacaaaata tcaaatttta tttatttgca tatattttct atatatttta
     7321 gccttttaac tttgtttttg ttttacatac tgtagtgctt ttctattctg ttttactgct
     7381 tcaagacaaa ctttttctgt ttgattatca acaaattcta aagctctata attttgcttt
     7441 actgcttcta aacatatttt ttctgtttga ttatttacat atttcaacat tctataatct
     7501 tgttttactg cttttagaca aacttcttct gtttgttctt tcacatattt aagcgcttca
     7561 taactttgat taactgcttc taaacaaatc tcttctgttt gttcctttac ataagctaaa
     7621 gcattatagt tttctcttac tgcctctaaa catattaatt ctgtctgatt ttctacatat
     7681 ttaagaattt ttccattttg ctttactgct tctacaaata ttctttctgt tggtatcttt
     7741 acataactta atatttcttt tatttcaagt tcttcaaatg aacctcttac tgcttctata
     7801 catatatttt cagtctgttt ttttacatat tgcaatactt ttccattttg tctaactgac
     7861 tccaagcaaa tttcttctgt ttgctctttt acgtactgta atgctcttcc atcaattctt
     7921 actgcttcca aacaaatctc ttctgtttgc ttatttacat actgtagagc catataactt
     7981 ctttttactg cttccataca tattttttct gtctgttcat ttacataata caatgcaaag
     8041 tcattctgtt ttactgcttc tatacatatt tcttctgttt gctcttttac acaagatagt
     8101 gcactgtagt tttcttttac agcttctaaa catatactat tactttgttc ctttacataa
     8161 gctaaagatt ttccatcttt ttttacagct tctaaacata tggctggagt ttgctctttt
     8221 attcctttta atatctctgc aacatcattt ttagatgccc tttctataaa tgctagacat
     8281 acatcttctg tttgatcttc cacaaattta aatacttcca tgacatcata agttgatgca
     8341 ttttttatag cctcaattag tacattttct gtcttattat ttatgtagta caaagctctg
     8401 tagttctgtt ttactgcttc tatacaaata tcctcatttt gatttgttat atacttaaga
     8461 gctttatatg tctgttttac tgcttccata catactttat aagtcttgtc ctttacaaac
     8521 tcaattatag taccatcctg ctttactgct agcagacaca tctcttctgt ttggttttct
     8581 atgtctttta actctctgta atcatatttt actctgctta aatcatttaa atgtactttg
     8641 ttatcttcct ttaaatcttc ttcattattt tctatatcat actcaaattc ttcatcatct
     8701 aaatagtcat ttttttctgg taaaaatata gatttaattt tattctttat atccataaaa
     8761 ttatcctcct ttaatatgat tctatcatta aagtaaatcg tttttaagca cttatttaat
     8821 taattttata ttaagtttat ttaaaagtct attcattgtc gatagattat attttatatt
     8881 aataatttta tcttatatat gtggggtaat aaagttaata gtggacattt tccat
//
LOCUS       DAMJVX010000138         8901 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMJVX010000138 DAMJVX010000000
VERSION     DAMJVX010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8901
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.143"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            84..425
                     /locus_tag="PC407_002827"
     CDS             84..425
                     /locus_tag="PC407_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HDF2498254.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            576..989
                     /locus_tag="PC407_002828"
     CDS             576..989
                     /locus_tag="PC407_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HDF2498255.1"
                     /translation="MIELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNLYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1191..1832
                     /locus_tag="PC407_002829"
     CDS             1191..1832
                     /locus_tag="PC407_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2498256.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1856..3109
                     /locus_tag="PC407_002830"
     CDS             1856..3109
                     /locus_tag="PC407_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2498257.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEKENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLREIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3155..3805
                     /locus_tag="PC407_002831"
     CDS             3155..3805
                     /locus_tag="PC407_002831"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDF2498258.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGVDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKSSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3856..4452
                     /locus_tag="PC407_002832"
     CDS             3856..4452
                     /locus_tag="PC407_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2498259.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4465..5304
                     /locus_tag="PC407_002833"
     CDS             4465..5304
                     /locus_tag="PC407_002833"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDF2498260.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFNNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5549..6703
                     /locus_tag="PC407_002834"
     CDS             5549..6703
                     /locus_tag="PC407_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HDF2498261.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKFYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            7009..8706
                     /gene="ade"
                     /locus_tag="PC407_002835"
     CDS             7009..8706
                     /gene="ade"
                     /locus_tag="PC407_002835"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDF2498262.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIASLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT         3640 a          860 c         1581 g         2820 t
ORIGIN      
        1 atcagtctta catatatttc cgatagatta tattgataaa aaacactaaa attaaattaa
       61 taaaagtact tggagggatg gaaatgaaag aggaaaattt tattaatgtt gaatacgagg
      121 ataaacgttt ttcaattaat agtaaagaag ttttgtacat agaaaagcaa gagtatgaag
      181 taaagattta tactgaaagt ggtaattatg aagttggaga aaatgctcct tgtataagaa
      241 aactattgaa agaagaaaat tttttcaaat gcagtaatag ttatattgta aatctagaca
      301 agtgtgaatt ttatctcagc aataattgta tttttgtgaa taataatttt ataagaattt
      361 taggttcaga aagaaaaaag aaattaaaaa agaaattaga aaaattatat gattatgaag
      421 attaatattg ggaggctgta ttttatacag cctctttatt tattgattgt attattaata
      481 aattttaaat tatgataaaa tataaaacaa gtaacttatt tcagaataaa atacttttta
      541 aaagaaaggg acgagatatg ggagattata ataaaatgat tgaattaaag aataacttag
      601 gtatcagtga aagaaagtta aaatatcctt gtatctataa acattttaaa gggaaatatt
      661 atgctacaat gggtcttagc aaggctattg atgatataga aaacatatgt gaaatatatg
      721 gtaaagaaaa tttaatccaa aacagaaata aatataaatt agttattaga catactgaaa
      781 gagaagaaga tatctatgtt tatagggatt tagatggaaa cctttatcat aaaaaagaag
      841 aagatactaa tgatttagtt ttatacaaga ccttatatga tgatacagga atatttgcaa
      901 gacctttgga tatgttttta gaaaaagtag atacagataa gtatgtcaat tcaattcaag
      961 aatacagatt tgaagaagta tataaataat ataagagaaa tatgtttata aatttagaaa
     1021 aattctttaa cagtacaata atttgcagaa atacaaatga gatttattgt aaataatatg
     1081 ttagattaga ttataaaata gaaacaaagt aaagtattat atgatataat taagttttga
     1141 gaagtgataa tttatattta ataaaaaata ggcatatatt aggaggacat atgggaaata
     1201 ctttttatat agtaagacat ggacaaacgg attggaatat attgggaaaa acacaaggtc
     1261 atggtaattc tgaccttacg cctcaaggta tagaacaggc aaaagaactt tctgaagata
     1321 ttggcaagta ttcaattgat tatattttta gtagcgacct tgggagagca atgcaaacag
     1381 ctcaaatttt aggagataag ctcaatattg aagttcaaaa aactgaggca cttagagaaa
     1441 tgggattcgg agtttgggaa ggccttttaa taaaagaaat tcaaaaagat tattcagata
     1501 tatatgcaac atggagaaat gaaccacact tagtaaatat accagaaggc gaaactctta
     1561 agattataaa ggaaagagta gatgctttta taaaggaact aaatgaaaaa tatgataata
     1621 aaaatataat tcttgttaca cattcaatta cattgagggt tatgttgttg tcatttttag
     1681 aatcaggtat ggaaaatata tatagaatta agcaagataa tactgcttta aatatagtag
     1741 aatttaaaga ttatggacct gttatagtaa aaatgaatga tacaagtcat ataaaaaatc
     1801 atgtaaagat aaataattct gcactggagt aggaatcaat tataaggaga aatttatgag
     1861 taaagttata gtagttggtg gaggagcttc tggaatgatg gcagctttgt cagcttcaaa
     1921 aaacaataat gaagtaatat tagttgagag aaatggagaa ttaggaagaa aacttagggc
     1981 aactggtggc ggtagatgca attttaccaa taatagagag atagaagatt tttttgataa
     2041 agttgtcagt aataaaaaat ttctctatag tagtttctat acatttacaa ataaagacct
     2101 tatttcgtat tttgaaagta gaaatttgga atataagatt gaaaaagaaa atgaccacaa
     2161 agtatacact aaaaatgata aatctataga agtaatagaa gtattaaata aagatttact
     2221 caatcacaat gtaaagataa tgtataataa gaaagttata gatattataa ctgaagaaat
     2281 tgctctaaaa gatgattcta ataaggacaa gtcaaagtat ctaataaaag ggattatttt
     2341 agataatgga gataagatac taggagacaa ggttattata tcaactggag gagtgagtta
     2401 ttcaaagacg ggttcagatg gttcaatgta taagattctt aaaaagcatg gacatacatt
     2461 aaacaaacta tatcctgctt tagttccact tacaatagag gaaaaatgga ttaaagattt
     2521 acaaggaata tccatgaaga atgtggaaat atcttgtaag attaagaaaa ggaaaatatc
     2581 aaaatctgga gacatgctat ttgcccattt tgggataaca ggaccttgtg ttcttataat
     2641 gtcttcttat attaacaaga ttattgaaaa ggaaaaggta gaattaaata tagatttctt
     2701 accaaattta agtactgatg aaatctctag tataatcaga gcatttccaa ataaaaatgt
     2761 tctaaataat cttaagcaaa tactacctca aaatttctta agagagattt tttctctatt
     2821 atctttagtt gataaaaagg caagtgattt gtctaaggca gatgaaatta gaattattga
     2881 gtatataaaa aatatgaagc taacttgtaa tgggactaca ggtataaaca ctggtatggt
     2941 tacttctggt ggtatttcag ttaaagaaat taattcttct actatggaat ctaaattagt
     3001 taagaactta ttttttacag gtgaagtaat tgatatagat gcagaaactg gtggatataa
     3061 tttacaaata gcattttcaa ctggatatct tgcgggtata agtgtataaa tataattttt
     3121 taataattaa atatgaataa atagaggtgt atatatgggt aatttagtta tagctgtaga
     3181 tggtccagca ggagctggaa aaagtacaat agccaagata gtagcaaaaa agctaaatat
     3241 aaattatata gatacaggtg ctatgtacag agcagttact tataagtgtt taaaaagtgg
     3301 tgttgatgtc aataatgaaa aagaagttat tcaaatagct gaaaattcgg atatagattt
     3361 taaagataac aatatatatt tggacaaaga agttataaat gaagaaataa gaacaataga
     3421 agtaagcaat aatgtttcta atgtagcaaa aataaaagaa gtaagacagt tgatggttga
     3481 agttcaacga aaaataggaa tgaaaagttc agtaatacta gatggtagag atataggttc
     3541 atatgttttc ccagatgcag attacaaatt ctttttagta gctactccag aagaaagagg
     3601 aaatagacgc tataaagaac tttgtaataa aggatataat actactttag aagaagtaat
     3661 tgaggatata ataagacgag atgaaattga ttcaaataga gaatttgcac cattggtaaa
     3721 agctaatgat gcactagaaa tagatactac aggaaaaact atagaagaag ttgtagaaga
     3781 agttgtatct aaaataaatc tttagttttt tatgcatcat aatgaaaata aatgtagtgt
     3841 tgaagggaga taaacgtgaa tttttataga tttgtaataa atatttttaa gggtttttct
     3901 aaaatatttt ttaaatatga agttataggt gcagagaata ttccagatag aggaaatata
     3961 gtaatagcat caaatcataa atcgaatctt gaccctatat tcttggctgc tgccatagaa
     4021 aatagggaaa tagctgcaat tgcaaagaaa gaattgttta aagtaaagcc attgggattt
     4081 atattaaaaa aattgcatgt tatgcctata aatagagaaa aaccagatgt atctacaata
     4141 aaaactattt taaggtcagt tagagatggt tatgtattag gaatatttcc agagggtact
     4201 agaataaaag gagattcttt tggtaaggct aaggcaggtc tttctgtatt tacaataaaa
     4261 agtaaatcta aagttgttcc agtatcaata atatctaaat ataaactttt tagtaaagta
     4321 attgtatata ttggagaacc aatatcattt gaagaacatt ttaaggaaaa attgagtaat
     4381 gatgaccatg aaaggatatc acaagagatt ttagaagtga taaaacaaaa ttactttaaa
     4441 tattcaaaat aagggggtaa agttatgaac ataaaaatag caaaaaatgc tggcttttgt
     4501 tttggagtaa aaagagcaat gaaaatggca tgggatgaag ttgaaaaaaa tgattctggc
     4561 atatatgctt taggtcctct tattcataac aagcaagcag tagcaaaata tgaagaaaaa
     4621 ggtcttaaga ctgtgaatga aattgataca ataccaaatc atgaaaatat gataataagg
     4681 tctcatggag ttcctgaaaa tatatataag gaagccaaag ataaaaaatt aaaaattgtg
     4741 gatactacat gtccatttgt aaagaaaata catacagtag taagtgaata tcataataaa
     4801 ggctatgaaa taatagttat tggagatatg aagcatccag aagttatagg tataaatgga
     4861 tggtgtgaaa attcagctat aataataaag accttagaac aaatggaaaa tatggaattt
     4921 aataactcca aaaaatattg tcttgttgca cagactacaa taaatccaga gttgtatata
     4981 agtatagtta ataaactatc agataaatta gaagaaatag tatttaatga tacaatttgt
     5041 tcagctacaa aaacaagaca agaatcagct aaagagttag ctaaagaagt tgattgtatg
     5101 atagttatag gaggtaaaca cagttcaaat actcaaaagt tagtaaaggt ttgtgaagac
     5161 ttagtaccaa cttttgcaat agagactaaa gatgaattgg atgtaaatac gcttaaaaaa
     5221 tataaaaatt tgggtattac agcaggggca tctacaccta attggataat agaagaagtt
     5281 gtaacattcc ttgaaaattt ataatttgat aggtaataaa aataggtgga cattgttata
     5341 atttatcata atatccacct ttgatttcat attacatgtg gtaaatagat aacctaatat
     5401 caaaaagtgt gtatcttaag atgaaaaact aagagtataa gatgcgcact ttttaatatt
     5461 aaaaattcaa aagaaatatt gatattgatt atcaatagaa aatagaatat aatataattg
     5521 ttgcaaaatt agtctaggag gtaaattaat ggaaatgttg atatccgctt ctgaagaggc
     5581 gtttttacat gtaggttcta tgattggatt ttttattttg ttatttggat atattaatta
     5641 taaaactagt ggaaatttta ctaatatcat atcaaaaaac agaaaatttc aacccttaat
     5701 aggtgcttta attggagcta taccaggttg tggcggaagt ctggcaataa tgccattgta
     5761 tataaatgga aaattaagct ttggagcaat aattgcaagt ttaatagcta gtatgggaga
     5821 tgcagcattt gtacttattt catcaaatat taaaatgtat ttttttgtca ctatagttag
     5881 tacaattaca ggaataataa cgggacaatt agtggattac tttaaactag aagaaaaatt
     5941 agggttaaaa aaccgtagaa aatcagataa atatgatata agtaaaaata accctaaaga
     6001 aaataaagaa caacataatc atgatgaaat aattttagac actctagcta aatctcatgg
     6061 aaatacaaat agacttgcat ttataataac acatggaaag ggatataaat tttatatagg
     6121 aattatagta atcggtttta tatttatgtc acttgctcac tctggactta atcttcctat
     6181 aattgagaag ttacattcat tggaagaagt aatagcagtg attggaatat tattttcaat
     6241 aatttatatg tggtgtttta aaaaggtatt taaaaattct aatcaaaaag aggaagaaaa
     6301 taaaaagatt tctttgcgag aaatgcttat ccattctgtt ggagaaatat catttgtaat
     6361 tacatggatt tttattgcat atcttattta cgatttgata atattaattt taggtggaga
     6421 tgaatactta gttaaactag tattatcaac aggtgtaata agtgtattta taggtgcagg
     6481 tcttggactt attccaggtt gtgggataca aattgtgctt atgtcatttt atttgaaggg
     6541 aactatacca cttggtgcag tgatagccaa ctctatttct caagatggag atgcactttt
     6601 cccactttta gcaatggata aaaaatcttc tttatgggct atgattatta ctactatacc
     6661 agcaatactt gtaggtatga tagtttatgc atttttaggt taaatataat tacgaataaa
     6721 gaagtattaa aaaaataata aagcacataa atttatttat aagaatagtg aagattaaat
     6781 ataaaaataa aaaattcaat atgaattaaa ttattgctta aatattaatg agaacatggg
     6841 agataatatt attcctcatg ttctctctat ttaattaaag gaattatgga acaaatttca
     6901 agtgatactt tctatcaaaa ctcatatata atatatatta ataaattaaa ctataatacg
     6961 taatacagcc agtatattga tataaaaaac ttaggaggag ttgtaaaaat gggtaattta
     7021 aaaagacagt gtgatgtttc aagtggaaga gaaaaagctg acattgtact aaaaaatggg
     7081 acaatcataa atgtatttac agaagagtta ataacaggag atgtagcaat agtaggagac
     7141 actatcgttg gcattggcga ttacaagggc aatgtagaaa ttgattgtag caataaatat
     7201 atatcacctg gctttattga tgcacatatg catattgaat ctacaatggt aatgccaatt
     7261 gaattatcga aaaaattatt aaaatcaggt acgacaacta tcatagccga cccacatgag
     7321 ttggtcaatg taaaaggagc tagtgcaata gattttttat tagaatcgac aaaagatata
     7381 ccacttaatg tatatattat ggtgccttca tccgtacctg caacaagttt tgaaactaat
     7441 ggagttggta aatttagtgc aaaagacatg gagagttatg taaataatcc tagaatttta
     7501 ggtttaggag aggtaatgtg ttttaatgat gttataaatt ctgaaaatga aatcttggat
     7561 aaactagagc tttttaaaaa taaagttgta gatggacatg cacctaatat aaatggtaaa
     7621 tcacttcaga catatgtatg tgcaggaata gaaaatgacc atgaatgtat aacttttgat
     7681 gaagtatatg aaaaattgag agcaggatta aaaattttaa taagagaagg aagcgcagca
     7741 aaaaatttaa agtctatagt aagtggtatg ctaaagcata acttgccaat tgaagaattt
     7801 atgttttgca cagatgataa gcacttagat gatatagaaa aacaaggtca cataagatgg
     7861 aatataaaat gtgcgattga tttaggtatg gaacctgtta gggctataaa agttgcaact
     7921 tataattctg caaaagcata tgggttaagg aaaattggag caataggtgc gggttataag
     7981 gcagatattg ttgtattgaa tgatttagac aaaatggaag tggatagtgt atataaagat
     8041 ggtaatttgg taaacgaaga gatgttttct aattacaatt atgagattaa agataaagaa
     8101 ttgcttaata cagtcaagtt taagtatatt aataaagaaa aaatacagtt aaaagtaagt
     8161 gaaaaaaatt atgtaatgga gattgtacct tatcaaattt tgacaaataa ggtatatgaa
     8221 tctttaccat gtgcagatgg atattttgtt ccaaataaag agtattcaaa actttgtgtt
     8281 gttgagagac atagaatgac tggaaatgta gcaattgctt cactaaaagg atttggcata
     8341 aaaaatggag caattgctac tactgtggcc catgattctc ataatattat agttgcaggg
     8401 gataatgatg atgatattct agtagctata aattacttaa aggaaataca aggtggttat
     8461 gtaattgtat caaatggaaa agttcttgca cacctaagtc ttcaagtagc tggattaatt
     8521 agtactttta cagctgaaga agtacaagaa attactgata atatgttgga gatagcaaga
     8581 aaaatgggag ttccagagta tgtagaccct tttattacac tttcttttat ggcacttcca
     8641 gttgttccac aaataagact tacagattta ggtctatttg atgttgagga atttaaattt
     8701 atatagataa tataaaagta ataataaatg aggctttctc aaaataggga taagtctctt
     8761 tttattgttg aaatgatgaa aatatatttg atttcataca aaaaagagca tctaatgaac
     8821 taaaaatttt atttttagat atccttattt taattaatct atcatttaac cttgttttaa
     8881 gcatagatat cttaataaaa c
//
LOCUS       DAMJVX010000139         8818 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMJVX010000139 DAMJVX010000000
VERSION     DAMJVX010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.144"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            230..1309
                     /locus_tag="PC407_002836"
     CDS             230..1309
                     /locus_tag="PC407_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2498263.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDEINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYMKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRRH
                     YPGIHSITIIINGVEKGKLDFELGV"
     gene            1633..2001
                     /locus_tag="PC407_002837"
     CDS             1633..2001
                     /locus_tag="PC407_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF2498264.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2118..2921
                     /locus_tag="PC407_002838"
     CDS             2118..2921
                     /locus_tag="PC407_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2498265.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFMPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPSELNQIMDIFIKQLNI"
     gene            3306..4196
                     /locus_tag="PC407_002839"
     CDS             3306..4196
                     /locus_tag="PC407_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2498266.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSARFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTIQNKDSCEKLLREECARYGILTD
                     MKEIIKAYKRSESYIQIKFF"
     gene            4659..5102
                     /locus_tag="PC407_002840"
     CDS             4659..5102
                     /locus_tag="PC407_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2498267.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            5274..5444
                     /locus_tag="PC407_002841"
     CDS             5274..5444
                     /locus_tag="PC407_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498268.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            5995..6903
                     /locus_tag="PC407_002842"
     CDS             5995..6903
                     /locus_tag="PC407_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2498269.1"
                     /translation="MTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFINDI
                     LKDAPYANRNIKPFTHIKRIIKAETDPTINYIGEKRPRLYILAEDEESNHINIEMQRA
                     LEDDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLTM
                     DGHPNIYSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIKR
                     ALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGVEKGKREEKFNIIIKLKNK
                     GYNLSEICDIIDDLNKSEVEKIYNQN"
     gene            7083..7571
                     /locus_tag="PC407_002843"
     CDS             7083..7571
                     /locus_tag="PC407_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HDF2498270.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            7657..8064
                     /locus_tag="PC407_002844"
     CDS             7657..8064
                     /locus_tag="PC407_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498271.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            8065..8283
                     /locus_tag="PC407_002845"
     CDS             8065..8283
                     /locus_tag="PC407_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2498272.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
BASE COUNT         3487 a          872 c         1547 g         2912 t
ORIGIN      
        1 agtaaagtat aaaattctat tactataata aaagatagaa ctagaagtct ttatgaaagt
       61 gggaaaagta tttatataaa taaatttata tggtttatag tttaacagta ggaaatatcg
      121 tatgagtgag aatatcgtat tttacataaa gatttagcaa tatgacacac tgttgtcgtg
      181 ttctgtagga tatgattaat aaatcagata tgaaaggaga gtgagtaata tgccagaatt
      241 actaaaaaat atgtataatc gtgagtcact ttatgaagtt gcagtagcta tccaatcagt
      301 ttacaactca tttaaggtag acgagttcat aaaatctaca atggatgaga catggaataa
      361 cttagaatta aaagccagat gtcgaaagat aagtatgagt ttaggaatgt atttaccaga
      421 agactataag gaagctcttt ccattcttga aaaatcagtt acaggttttt attttgcttt
      481 tttctttcca gattttgttg aagtttatgg gcaggacgaa ataaattggg atttatctat
      541 aagtgcatta gaaagaaata ctgagtattg gtcttcagaa tttgctgtaa gagcatttat
      601 catcaaggat gaagagcgta tgatggcaca gatgcgtaaa tggtctaagc ataagagcga
      661 acatgtacga agacttgcaa gtgaaggttg tcgtccccag ttgccttggg gacaagcaat
      721 atccaaattt aaaaaagacc ctacacctgt actgcctata ttggagcaac ttaaaacaga
      781 tacatcaacg tatgtacaaa agagtgttgc taataatcta aatgatatct caaaaacaca
      841 tccagattta gtcataagca ttgcaaagga ttggtacggt aaaaataaat ctactaactg
      901 gattgtaaag catggttgta gaactctact aaaaaaaggc aatcgtgatg tattagccct
      961 ttttggatat gatgatacta cttctataaa tcttcaggac tttactttag aaacaacgtc
     1021 tatttctatt ggagaggatt taactttttc atttaatatt ttagcaaaaa aagcgacaaa
     1081 aacaaggctc gagtacggaa ttgattatat gaaatctaat ggaaaaagga ataggaagat
     1141 atttaaaata tcggaggttt cattaaaaga aaatgagaaa aagtcatata tgaaaaaaca
     1201 ttcatttgca gatgtaagtg taaggagaca ttatccaggt atccactcga ttactattat
     1261 tattaatggg gtagaaaaag gcaaacttga ttttgaatta ggtgtataat tttactaaca
     1321 gcataaccta tatcagatac tttgctaaac ctatatcgta tactctaata aatctatatt
     1381 aggtagttta ctacttgttt actgaattta aatacaaaga aatacaggtg ttaatccctg
     1441 cccattaaag gaatatgagc ataacacaaa agtagaactt atcaattctg ttacgggtat
     1501 agtagtaaat ccatagaatc acaattagaa gatgaagtgt aaaaaataag ttagtgttga
     1561 ttttagttgc tagtgatatt gaatagttat acaatagcca tataaattag aatttgtaga
     1621 ggagaaatgt aaatggatgt atatagtttt tggaatgaca ctttggcaca agatgctgtt
     1681 aagataagaa aatattttca tgaagatgct tatattaatt ggcatgatac aaatgagcat
     1741 tttactttag aagaatttat tattgcaaat tgtgagtatc ctgataaatg gaatggtaaa
     1801 gtagaaaggg tgctagaatt tggaaattta gtaattacag tgacacatgt ttatgcaata
     1861 aataaaccac tatcatttca tgtcgtttca tttatcagcc ttaaagatga taagattatt
     1921 tctattgatg aatattgggg agaggatgat gatgtgccac agtggagatt agataaacat
     1981 attggaaagc caataaaata gtgtttgttc tgttttataa ctgaaatttt gataacattt
     2041 gatattttga tttgtcattg gtcaacatag tcgttagtgt aataatttaa aaaagtagaa
     2101 tttggagagt gagcactatg gaagaaaaat acatcttatc tgaccgaggt aaagtatatt
     2161 attggataaa agaaagtaat agtgtgtttg caaagaactt aatttttttg ccaggactaa
     2221 cagcaaatca tcatcttttt gatttccaga tacagtactt ttcaaaaaat cataatgtat
     2281 tggtatggga cgctcctgct catggaaaat caagacctta cttagatttc tcatattcta
     2341 acttggtaga agaattgaaa gtaattttag ataaacaaaa tatgaaaaag gttgtactta
     2401 tagggcaatc ggcaggggga tttgtggcac agtcctttat tgtaaaatat ccaaatatgg
     2461 tagaaggatt aatgatgatt ggaacttgtc catatggaac tgactattat agtaagtcag
     2521 atattttctg gttaaaacag actgaatgga tgttaaagtt attcccagat aaattgttgc
     2581 gaaacataat atctaagatg tgtggagcta cgtctgaagg cagaaggagt atgatgaaaa
     2641 tgttagaagg ctacgaaaaa aaagaactgt gtcatctcat gtatctggga tttgctgggt
     2701 tcatgccaga aattcaagac ttaaatatac attgtccagt ttgtttggtg gttggagata
     2761 aagacaagac gggtaaagtg agaaaatata atgagcagtg gcacaataag actgggtatc
     2821 cattgcacat tataaaaaat gcttcacata atgtaaatgt cgataaacct agtgaactaa
     2881 atcagattat ggatatattt attaagcaat taaatatata aaaactacat actcattagg
     2941 taaattatgt agtatatgtg tttacatatg gttatagaaa taaatatgta ataaactact
     3001 taacacttaa aaagtggatt tgtccagagc ataatacaaa gcatgatata taaattttta
     3061 agcgaaatac atcaaaagaa gtattagaat tagtggagat aaaacagcta ttagagatta
     3121 taaataaata gttttaaagg tactacgaat atagaggaag gtaaatcata cgttattcaa
     3181 ggattataaa tgagtagttt aaaagtacta tcggataggt ctctgcatat ttaaacctta
     3241 gtaaaggtag ttaaagtagt agtttaaaaa ctaaaaaatt atataaagga attaggtata
     3301 agaaaatgat tgagagtata ccagcaaaac aaattttaca aaaagtgaaa tttgacaata
     3361 caagatggtt tggaattgac tataatatga atctctatcg tggttgttct catggatgta
     3421 tatattgtga tagccgtagc acaatataca acatagagaa ttttgacaaa gttagatata
     3481 aagaaaatgt gatagagatt ttaagtaaag aattgcgttc aaaaagaaaa aaaggggtag
     3541 taggcattgg agctatgtct gacacatata atccttttga aaagcaactt tgtattacaa
     3601 agcaagcact agatttgatt tcggaaaatc attttggagt gtctattgat actaaaagta
     3661 gtttggtggt tagagatata ccgattttgc agaaaattaa aaaaaataat tctgctattg
     3721 taaagttgac tataaccact gcaaatgatg aattatcaaa gaaaatagaa ccttatgtaa
     3781 atccaagttc tgcacgattt gacgcagtga aagaacttaa tgattctgga attttttgtg
     3841 gcatattatt gactcctatg cttccatttt tgactgataa taaagatgaa atacgagcaa
     3901 ttgtagaaaa agcacacaag gctaatgcaa agtttattta ttgcatgtat ggtgtaacaa
     3961 tgcgtagtgg tcagagagag tttttttatg agcatttaag agatatatca ccaaaattag
     4021 tacttaaata tcagaaaaca tatggtttaa actatgtatg tactatacaa aataaagatt
     4081 cttgtgagaa gttgttaaga gaagaatgtg cacgttatgg tattttgaca gatatgaaag
     4141 agattataaa agcgtacaag aggtcggagt catatattca aataaaattt ttttaagata
     4201 ggctttggat gagatataat agtggtgatt gatgtgttga tattatagtt gattgttaga
     4261 gaggatatta tagatgaata ttaatattga tattctaggt aattgctaga actaatatta
     4321 tagatgaata ttagtattga tattctaggt aattgctaga gttagtatta tagatgaata
     4381 ttagtattga tattctaggt aactgctaga gctaatatta tagatgaata ttagtattga
     4441 tattctaggt aattgctaga gctgatatta tagatgaata ttagtattga tattctaggt
     4501 aattgctaga gctgatatta taatttatta ttaatattaa tattctaggg aattatcact
     4561 atattttaaa aagtaaaaat atatatcaaa atgaactaaa taagtactgt aagaagtttg
     4621 ttaaattaag gtttgaataa agtgagggtg gagaacttat gatagaaata aaaactatag
     4681 aaataaataa aaaaagtcaa attgaccttt taatgttagc cgacccatgt gaagaaatga
     4741 ttgataaata tctcaataaa ggaactatgt acgcattgta tgataataaa gaacttactt
     4801 gtatagcagt tgtaaatgaa atttccaaag aaatatgtga attaaaaaac attgctacat
     4861 atgagcattt tcaaaatatg ggttatgcct caaagatgat ttatcatctt ttagatgttt
     4921 atagtaaaaa atattcatca atggttgttg gtacatctga aagcggagtt cctttttatg
     4981 agaagtttgg atttgtttat tcacataaaa ttaaaaactt ttttgtagat aattatccag
     5041 aaccaatctt tgaaggagaa ttacaatgtg ttgacatgtt gtatctatca tataactttt
     5101 agataggata atatttttag gtagatataa ttaattaatt catataatta tcggaattat
     5161 aatttataaa ttatgtataa taagagtata atttatttag aagctgttat attgtattat
     5221 attcaaataa gatatataat aatttgttta ttttgaattt tggaggatta attatggatt
     5281 caaataataa tactataaaa tcaactgtta aaaagggtat ttcttttggt tcttgtttag
     5341 caatgattat ttcttatact gcatggaaat ctattccatg ggctattttt catggcttaa
     5401 tgagttggat atatgtactt tattattggg ttaagtatgc atagattaaa ttaataaata
     5461 agtatgtaat attgtaaagt ttaagaaaca aaatgtaagg gagtgtctcg aagtgaactt
     5521 tttagtttat aaggcactct tttttgtatt aaataaaata tattttcatt acttaaacaa
     5581 tgaaaaagat gagtatctct attttaatac atacccttat attcattcat ttatcaacaa
     5641 tagttttaaa aagaaagcta tctaaaatct attgtaatct aagtgcttct ctttaaagtc
     5701 ttaactttag taatgatata taagtttata aggttcttat ttcaataatg caaattttac
     5761 ttagaagttc agactttggt atgaagaaat gggtaataat aaaaaagagg tgttgatatg
     5821 aaagaaaaat tagaagaaaa atctaagatt attaaaacat caaaagagta taaacgaact
     5881 ataattgaag aaaaggacag agaatgaacc aaaaaattaa atgacatact tagtagaggt
     5941 gatttttcta atgaaaaatt agataaaatc aaggatttaa ttccaaaaga aatattgact
     6001 agtgaaaatg ttggagaagt tgtaactgaa gatgggtatg ctaagcctga gtatgatgag
     6061 gtatttaaat cattgtttac tttaaataat gattacgatt tacttgcaaa ttttataaat
     6121 gatatcctca aagatgctcc atatgccaat aggaatatca aaccgttcac tcatataaaa
     6181 agaataatta aggctgaaac cgacccaaca attaattaca ttggtgagaa aagacctaga
     6241 ctttatattt tggctgaaga tgaagaaagt aatcatataa atattgagat gcaaagagct
     6301 cttgaagatg attatttgga aagagctgaa tattatctta gtcgtgtaca tggtagaaag
     6361 ttagaagaag gtaaagagta taaagaaatt ggaaaaactg ttggaataca tatactaaat
     6421 catgtaaaat ataatcacat agaagattat gtaaattgcc tgagattaac aatggacggg
     6481 catccaaata tttattctag taaaactgca ttatatttta tagaacttcc aaaaattcgt
     6541 aaatccagtt gtatagcaaa cagagttcta atatggggta aacttattga taatccttct
     6601 catattgata ttagaatact atctaaaaca gattatgtta ttaaaagagc acttgataga
     6661 ttaaaagaat taggcagtaa tgaggactat ttattaaatt taaagagagg tgcgtatatt
     6721 atgaataaaa gtagaaattt taaagaagaa atttttcaag aaggtgttga aaaagggaaa
     6781 agagaagaga aatttaatat catcataaaa ttaaaaaata aaggatataa tttgagtgaa
     6841 atatgtgata ttatagatga tttgaataaa tcagaggttg aaaaaatcta taatcaaaac
     6901 taatactata attgagatgg cactagataa atataaagct tcctctttta aatttaaatt
     6961 aatataaaac cttgttattt aactatagta gatacaaaga gatatataag ctaaggagat
     7021 gattcaaaat aaaagtttga actatctcct tcttatagaa aaacaaaggg gttgatttat
     7081 atatgaaatt ttatactata cagactttag aattttggaa caacaataaa aacaataaat
     7141 atctatcaaa tgattattct tttatattag aagactttga tattccatac aaatggatgt
     7201 atagtcaaat gacaaataga atatatgaat ttgatgatag tatgatatgg gtttggacga
     7261 agagacctga tttaagatgt agtggttatt tagaaaaagg aaaaaaaggt gttttacttg
     7321 aaatagaaat aagtgaagac aatgtgttat tatcagaatt tgatttatgg catttacctt
     7381 taatggatat tactgtagag ctttatgaag atgaacaaat tgaaaagaag gtaagttggg
     7441 aaagaatatt tgacttagaa ctgtgttcaa aattaaatga agttaatata gaagatttga
     7501 cattgcaagg agtgacatct aaagtgcctc tatctaatgt aaaattaata aaagaatttg
     7561 ttgcaaaata atatttttat tgaaatttat tcaaaaaagt tatatattta taacaagagg
     7621 tgttataaat acataacttt ttaaggggga ttttatatgt ctaaacaaca aactggtatt
     7681 aaatcaacac ttatttcaga aatattggga atttcttatt gtattggagc tacattagta
     7741 aataagtatt tttttaaagg aagtagtatt gaatatcttc ctataattat ttgggtatta
     7801 gctatgggag ctcagtgggt ctataataaa aagtatgatg atttaattga tgaggcatca
     7861 aaatgtatac tatcaagagt taatgatatt gcaataaaag tattgttttt ttctgtagct
     7921 atagtatcta tatttcttgt aactttggac ataaatgtgt caaatttaaa tataggaatg
     7981 tctttattag tcattttgtt tattcaatcc ttgttgaagt taattctatt tatatatttt
     8041 gacagaaagg ggatatacaa ttaggtgtca gtattactaa agacaaaaat taaatcctat
     8101 agagaaaaga atcttatgac acaaggtgaa cttgcaaagt cagtaggagt aagacgtgaa
     8161 actattgtac atttagaaaa tggtcgctac aatccttctt taaaactggc aatggatatt
     8221 gctaaggtat ttgatacaac tgtagaagag ttgtttgaat ttattgaaga aaataatatt
     8281 taaatttcat gtattactta tgcattagag aatgaattaa tagataaaaa attcaagttt
     8341 atgtattaga gaatgaatta attataggta aaaaaccaag cttatagatt agagaataaa
     8401 tgacttatag atgaaaaacc aagctcatag attaaagaat gaatgattta tagatgaaaa
     8461 agaaagctta tagattagag aatgaattaa ttataggtaa aaaacaagct aatagattaa
     8521 aaaatgaatg atttatagat taagaagtaa gcttatagat tagagaataa atgacttata
     8581 aataaaaaga aagcttatag attagagaat gaattaatta taggtaaaaa acaagcttat
     8641 agattagaga ataaatgact tataaataaa aaccaagctt atagattaga gaatgaatta
     8701 attataaata aaaaccaagc ttatagataa gaagtccatg cttataagcc agaaaatgaa
     8761 tactttaata gattaaaaaa tacatatttg tgaattatag aatgaataac tccataaa
//
LOCUS       DAMJVX010000140         8783 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMJVX010000140 DAMJVX010000000
VERSION     DAMJVX010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8783
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.145"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            161..1222
                     /locus_tag="PC407_002846"
     CDS             161..1222
                     /locus_tag="PC407_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDF2498273.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGRKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNRLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            1313..2926
                     /locus_tag="PC407_002847"
     CDS             1313..2926
                     /locus_tag="PC407_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2498274.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            3201..5186
                     /locus_tag="PC407_002848"
     CDS             3201..5186
                     /locus_tag="PC407_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2498275.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLSFGILEKPVVWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMIEELAKVKSFDEFYKIITKRR"
     gene            5191..5625
                     /locus_tag="PC407_002849"
     CDS             5191..5625
                     /locus_tag="PC407_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDF2498276.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            5654..6214
                     /gene="srlA"
                     /locus_tag="PC407_002850"
     CDS             5654..6214
                     /gene="srlA"
                     /locus_tag="PC407_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF2498277.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            6278..6646
                     /locus_tag="PC407_002851"
     CDS             6278..6646
                     /locus_tag="PC407_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HDF2498278.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            6671..7237
                     /locus_tag="PC407_002852"
     CDS             6671..7237
                     /locus_tag="PC407_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF2498279.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            7278..7643
                     /locus_tag="PC407_002853"
     CDS             7278..7643
                     /locus_tag="PC407_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF2498280.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTNDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            7744..8517
                     /gene="srlD"
                     /locus_tag="PC407_002854"
     CDS             7744..8517
                     /gene="srlD"
                     /locus_tag="PC407_002854"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDF2498281.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIETEFGCKSVGYGCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
BASE COUNT         3509 a          892 c         1626 g         2756 t
ORIGIN      
        1 atacataaaa aataaaaaat tgtcgcttta aagtataaaa cagattatat gttaataatg
       61 tggtgaaatt atacccatat ataaataaaa taaaacaaat aaatagctaa ttaaggagaa
      121 aatatttttt attttttgtt taggataaat aaattttatt atggaaaaaa gaaaaagcat
      181 acatcttcac aaattgaata tttatttaat agggggaggt gatagattgt ttatcacagt
      241 ggtattattt ttagtaggtt tcctattaat tactaaaggc gcagatatct ttataaattg
      301 cactgtagag ataggtagaa aaacgaatat atcagaaatt atacttggag ctactatagt
      361 tagttttgca acaacattac ctgaatttac agtgtctcta cttgcttcta ttgatggtca
      421 tactactatg agtttaggaa atgcagttgg ttctataata tgtaatacag gattagcatt
      481 agggttggtt gtttttataa gtccatttaa tgtagataaa aaaatgtttt tttctaagtc
      541 tttactatta atagtatccg ttatagtttt aattttattg agtttagatg gagttataac
      601 aagaggtgat tcattattac ttataataat attaatcttt tatatggtta ataattatag
      661 aagtgtcgtt ggaaaatctg atactaaaaa tagaaatatt aaaaataaca ctattaaaga
      721 catatctact aaaaaaggga aaaattatag aggtttttcc gtattagaaa ttgtaaaaat
      781 actattactt tttgctacag gattgataat gatgataata ggttcacaaa tccttataga
      841 gagtggagta ataatagcta gttttttaaa tatacctcag ggtatagtaa gtttaactat
      901 aatagcactt ggaacttctc tgccagagat agtttcgtcg attactgcta taagaaaaaa
      961 tcatcatgaa atatcagtag gaaatatttt gggtgcaaac atattgaata tagtatcagt
     1021 aatagctgta tcagcaatac caaataacat accaatacta tctcaaaata gacaattgga
     1081 tataccattc atgatattat tattattaat agtaataata ccaacactta aatctaatag
     1141 acttagtaga atacaaggta tattgatgtt attcacatat tttctgtaca tatcaatttt
     1201 gtatttcatg tatattattt aactatactg tttaaaatat taattgcaga atatcctaaa
     1261 aaagtatata atggataggt taagattaat tgataacgga gaggaataat atatgaatat
     1321 aacaaagttt gaagatttac caataagtga gggaataaaa aaagctatag ctgaaatggg
     1381 gtttgaagaa ccatctccta tacaagcaca gtctatacct gcgattttgt caggtaaaga
     1441 cgttatagga caagctcaaa ctggtacggg aaaaacagct gcttttagta tccctatact
     1501 agaaacaata gacccaaata atagaagttt acaagctgta gtgctttgtc caacaagaga
     1561 actggcaata caagtatcaa cagaaataag aaagttagct aagtattcgc acggaataaa
     1621 gacattacct atatatggtg gccagccaat agatagacaa ataaaatctc taaagagtgg
     1681 tgtgcaagtt gtaataggta caccaggacg tacaatagac catatcaatc gtaaaacatt
     1741 aaagatggat aatgtaaaaa tgattatatt agatgaagca gatgaaatgc tagatatggg
     1801 atttagagaa gatatagaaa tgattttaag taagattcca gaagaaagac aaacaacttt
     1861 cttttcagct actatgccaa gaggaatttt ggaattaact aagagatatc aaaaagaccc
     1921 agagcatata aaagttgtta gaaaagaact tacagtttca aatacaaagc aatactatat
     1981 agaaactagg tcatcaaata aattagaagt attatgtaga ttagtagatg tatatgaccc
     2041 taaactatct gttgttttct gtaatactaa gagaaaagca gatgaattgg taggagattt
     2101 acaagcaaga ggatattttg cagatgcatt acatggtgac ttaaaacaga ctcaaagaga
     2161 tatagtaatg gataagttta gaaatggtac aatagatata cttgttgcta ctgatgttgc
     2221 agctagaggt atagatgttg atgatgttga atgtgtattc aactatgact taccacaaga
     2281 tgaagagtat tacgttcata gaattggtag aactggtcgt gctggtagag aaggtatgtc
     2341 atttactttt gtatttggaa aagaaatgag aaagatgaaa gatatagaaa gatatactaa
     2401 atctaaacta ataaagcata atataccaac aataactgat gttgaagaga aaaaagtagg
     2461 tactttcttt gcacaagtta aacaaactat agaagaaggt cacttaacta aacaattaca
     2521 atggttagaa ggtttctgta atgatgaaga ttatgcaatg gttgatatag ctgctgcact
     2581 tgtaaaacta tctctaggtg aagaaatgaa agaagaaata atagaagaaa aacctagaag
     2641 agagcgtgga gatagaaaag gtggtactgg agctaaagat ggtatgatta gattatttat
     2701 caatattggt agaaatcaaa gagtacaagc aaaagatata gttggtgcta tagctggaga
     2761 agttggaata ccaggtaaag tagtaggtac tatagatata tatgataagt atacttttgt
     2821 tgaaatacct aaaaaagatg caaaaacagt aattgaaaag atgaaagata ttaagataaa
     2881 aggtaataag attaatatag aaaaagcaaa taagaagaaa aaataacgta tagtatacaa
     2941 aactataatt agaaaatata taaaataata tattggatat ttaaaaagcc tgatgtgttt
     3001 tcaaaacaga tagtagataa tctgtaagag ttaacagcat aaggcttttc ttgttttata
     3061 tgatattatg attttccaga tcgttgtgaa aaatgtatat tagttccttt ttttcaaatc
     3121 tgatttgaaa aagagaatat taattatcta tatagtttag attataataa aaaatgtaag
     3181 ataacaaagg ggagaaaaag atgtcaatga cttatatgga ccctagggtt tttttgattt
     3241 tagaaatggt gttagaagaa gacacaaata ctctagaaca attttcaaat gttttgggtg
     3301 ttagtactag gactatcaga aactatataa accagattaa tcgagaatta aaaggtggta
     3361 ttgctaaaat agttaaaaat gaaaaaggta catatagtat aagaattgag gatgaaagta
     3421 aattaattga gattgtaaat tttaatagag gaaagctatc aaattttata aatttaaatt
     3481 cacctgatga aagaataaat tatgttcttg atattttaac tatgaccaac aatccaatta
     3541 ccatagatga tttggctgaa gagatatgta taggaagaac aacattaata aaggatttaa
     3601 aaaaagcaga cagaatttta gctgaatatg atttagaatt aaaaaggaag ccaaatatag
     3661 gtatgatttt agttggtgaa gaaatggata taagattatt aatccttgat agattgtatg
     3721 aaaattatat tgatgtcttg gaaaacgtta atcagataaa tttagttaaa gattatgata
     3781 ttgaatgtct tagagatgaa ttaaaaaagt tgtttaaaaa agaagaactc tacataacag
     3841 aacaggtctt gagagatgtt gaaagatata taatcatgtc tgtcttaaga aatctaaatg
     3901 ggtacaaatt tgaaaagata gataagagat ttgaagtgat aagatgttca gatgaatata
     3961 ctttaggatt aaagctcaaa gctttattgg aaaagatatt taatatagtt ttaaatgaaa
     4021 aagaaaccat atttttgaca atgccattaa taggtagaaa agctcctagt acaaaattag
     4081 cacttagcag tataaagata aatgatagcg taaaggaagt tgtagatgaa gtaactagtt
     4141 ttttattaga gacctcaggg attgatttta aagaagataa aaagcttata gaaaatttgg
     4201 aatatcactt gtattttgca cttaatagaa tgagattcaa cataagggta aaaaatcctc
     4261 ttttacaaga aataaaggaa agatatacat ttccatatag tgttgcaaag atagctgcaa
     4321 tgataattga ggaaaagttt gacttgaaaa tatgtgacca cgaaatagga tatatagcat
     4381 tacattttgg aagttatttt gaaaaaaatt caagaaaagt tctaaatgta aatagggtag
     4441 ctgttgtttg tggaacaggt cttgggactg cacaacttat tagaataaag ataggaaaaa
     4501 tacttggaga aagtgtagaa gtaaatactt tttctgatat agaagctaaa gctaatttag
     4561 atttattaaa gtattatgat ttagtattta caacagtaga tttcaaaaca gacttagatg
     4621 ttccaatttt tagagtaaat gcgttattta atgaagaaag tataaagaaa gagatagaaa
     4681 atgcaatact tttgaaaaat agtataaatc ttaagtctat aaaaaagatt gaaaaaaata
     4741 tcccttttat aggtaaatta attgaagagg ataagttctt tatattgaat aagagtacgt
     4801 ttatggaaaa tttagaagat atgttagaaa aattagttca taaaggaatt attgatatag
     4861 agttcaaaaa aaaggtatta aaaagagaaa agaaatcttc aacagcatta gataattata
     4921 tagcattgcc tcactcagtt aatactaatg gggaaaatct ttatctgtca tttggaatat
     4981 tagaaaaacc agtggtttgg gatacaaagg aaattagaat aataatattg atgataattc
     5041 cagataagaa tgtagatagt acagacctga taataaaaag ttatgaagaa gtacttgaag
     5101 tagggagaga taaaaaaatg atagaagaac ttgctaaagt aaaatcattt gatgaatttt
     5161 ataaaataat taccaagagg aggtaataat atggacccag taatttttat tatagcatca
     5221 cttattacag tgtatttatt aaatattttt cttggatact tacagcttaa agattttaat
     5281 aaaaattata ttgaactaaa aagaaaagga agagtggcaa taggcagaaa aaaaggaaga
     5341 atttcatcag gtacaattgt gttgatactt ataaatgatg atggtgtgat tgtcgaaact
     5401 agaaaaatgc aaggtgttac agtattggca agagtaaagt catttgaagg tttggtagga
     5461 aagagcttag agtacataga aaaatcagat ttaactgttt acaataaact acttaaaaaa
     5521 gcaatactag atgcagtcaa acagtacata acatttgaga aaaataaatg tgaagataca
     5581 aataatctga gtactgatga agagtgtagg atggaagtag tttaaaaaaa tatatttata
     5641 aggaggagtt aatatgacgc aattttttca agtattagca agtggtgcag atagttttat
     5701 gaatttattt agggcaggtg ggcaacaatt tgtaggtttt gtaacagata tagtaccact
     5761 tttagtagca ttattagtta caatgaatgc aattatcaac tttattggta cagatagagt
     5821 cgagagactt gcaaaaaaat gttctgcaaa tatatttact agatatttga tattaccagt
     5881 ccttggtaca tttgtatttg caaacccaat gacactatca ctaggaagat tcttgccaga
     5941 aaagtttaaa cctagttact ttgcagcagc aagcttttca tgccatacaa tgaatggatt
     6001 attcccacat atcaatccag gtgaactatt tatatatcta ggaattgcaa atggtataac
     6061 aactttggga ttctcaacag cagatttagc agttagatat ttgttagtgg gtatagttgc
     6121 aaactttatt aaaggtgtag ttactgacta tacaacaaaa tttgtagaga aacaacaagg
     6181 aatagcatta aattcagata taaagattgg ataattactt taaggtataa ataaattttt
     6241 caaataaaaa gatagtgtat agaggaggat aaattatatg gcatttaaca aagttaaaat
     6301 agtaaaaggt tctggaggat ggggtggacc attgataata gaaccaacag aaaagaagaa
     6361 taaggtagta tatgtgactg ggggagcaaa gccagaaact gcagtaaaaa tagctgaact
     6421 tacaggatgt gagttaatag atggattttc acagggtgtt aaagatgatg aaatcgcatg
     6481 tgtaataata aattgtggtg gaacattgag atgtggtata tatcctcaaa agaaaatacc
     6541 gactataaat ataatgaaaa caggaaaaag tggaccatta gcaatgttta taaaagaaga
     6601 tatatatgtt tcaggggtaa aaccaacaaa tgtagagcag ttataattga ggtagtagga
     6661 gggaagaaat atggcttatg atactaaaaa gaagataagt gagcagagtc aaggaattgt
     6721 tgcaaaaata ggtatagcac taggaaatgt gacaagtact ctttttcaag ctggtaggga
     6781 tactatagat acattaatac atactatact gccttttatg gctttcgttg cagtcctaat
     6841 aggtataata aatcaatcag gtataggaaa ttgggtagcc aatttactta ctccattaat
     6901 cggaaatgtt ggaggtttag ttgtcttatc aataatatgt tcatttccgt tgttgtctcc
     6961 gtttcttgga ccaggagctg ttatagctca gatagtgggg gtattggttg gtacagaaat
     7021 aggaaaagga aatattccac caagtttagc attaccagca ttgtttgcaa taaactcaca
     7081 agcagcttgt gactttatac cagtaggtct tggtcttgca gaagctgaac ctgatacagt
     7141 tcaagtagga gttccgtcag ttttatattc tagattttta acaggtgcac caactgttct
     7201 attagcatgg ttagcaagct ttgggttata taaatagaag aatactttat aaataattag
     7261 agagaggttc agttgatatg gaattaatat acagtactca aataaataaa ataggagata
     7321 atgcaaatga atttttacaa cataatatgt ttgtaacttt caaagataat gctcctcaag
     7381 agttagaaaa ttactgttat atacattctg aaaataattt agtaaaagaa atacttacga
     7441 atgatgtgtt atcaataaat aatgttgatt acaaaattac ttctgttggt gagttagtaa
     7501 accaaaatct ttcagaatta ggtcatataa catttaagtt tacaggtgaa aaagatggag
     7561 caattggagg gaccttatat cttgaagaaa aagaaatagc acctattggc gtaggtacta
     7621 tactaaaaat aattagaaaa taaaatttaa aacgaatcta atcatattat aagtaaatta
     7681 tagataaaag tcaatttaac taatcaagtt aacttttaat gtgataaaaa aggggaaatg
     7741 aaaatgagaa aagtggcttt agttgttgga ggaggaagaa gtttaggtga atatttatct
     7801 aaacatttag ctaaagtagg ttatgatgtt gctattgcag atttaaatga agacaacgca
     7861 aaaaaagtag ctgaagaaat agaaactgaa tttggttgta aatctgtagg atatggttgt
     7921 aatgccatag atgaaagaca ggttataagc actgttaaag ctattgaaaa tgattttgat
     7981 agaatagatt tattagtgta caatgcagga gtggctgtaa gtaaaaaaat aactgatttt
     8041 ggatacaaag attttaaatt tattgtagac gtaaatttaa atgggtactt tctatttgca
     8101 agagaagtat ctaaagtcat gatagcaaaa ggtattgagg gtagaatcat acagataaat
     8161 tcaaaatcag gtagagtggg aagcaagcat aattcaggat attcagcagg taaatttgct
     8221 ggagtaggac ttactcaaag tttagcgcta gatttggcag aacataagat aactgtaaat
     8281 gcactgatgc tcggtaattt acttgattct gatatgtttg aaagtttaat tcctcaatat
     8341 gcagtaaaat tgggaatacc tgaatcagaa gtcaagcaag tttatataga taaagtacct
     8401 ttaaaaagag ggtgtagatt tatagatgtt gcaaatatgc ttacatttta tgcttcaaag
     8461 gaagctgaat actgtactgg tcaatctata aatattactg gtggacaagt tatgtaaggt
     8521 ttttatggag tgtctcaaga tgaactcttt agttcatgag gcactttttt tgtataaaat
     8581 taaatatatt ttcatcattt aaacaatgaa aaagaggttt atctttattt tgagatagcc
     8641 tcatttttgt aattaagaaa catagtgttt aagtaaacat ataagattgt tagtttttta
     8701 aaagagaatt ttttaataat attatattta tgaagtattt ttaaacttaa tttgattaaa
     8761 aaatagctac gcacacttgt gac
//
LOCUS       DAMJVX010000141         8737 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMJVX010000141 DAMJVX010000000
VERSION     DAMJVX010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8737
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.146"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(70..1842)
                     /locus_tag="PC407_002855"
     CDS             complement(70..1842)
                     /locus_tag="PC407_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF2498282.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYNMDKHPALDSMTRRIVNDIRNIDTN
                     QDMMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(1856..2785)
                     /locus_tag="PC407_002856"
     CDS             complement(1856..2785)
                     /locus_tag="PC407_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2498283.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            3014..3202
                     /locus_tag="PC407_002857"
     CDS             3014..3202
                     /locus_tag="PC407_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2498284.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRAKRRKEVQYE"
     gene            3195..3896
                     /locus_tag="PC407_002858"
     CDS             3195..3896
                     /locus_tag="PC407_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2498285.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLLDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSINNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            3898..4821
                     /locus_tag="PC407_002859"
     CDS             3898..4821
                     /locus_tag="PC407_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2498286.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            4978..5901
                     /locus_tag="PC407_002860"
     CDS             4978..5901
                     /locus_tag="PC407_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498287.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGCEKRVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDHTLRLYNTTLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            5894..6631
                     /locus_tag="PC407_002861"
     CDS             5894..6631
                     /locus_tag="PC407_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2498288.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFASIALGRRK"
     gene            6642..7415
                     /locus_tag="PC407_002862"
     CDS             6642..7415
                     /locus_tag="PC407_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2498289.1"
                     /translation="MWKLLWAECQKIRRSKIVWITFFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            7431..7583
                     /locus_tag="PC407_002863"
     CDS             7431..7583
                     /locus_tag="PC407_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498290.1"
                     /translation="MKKIKKLIILIVFLFLFGMLHCYTEWKFNNDPPKGVIERINEWT
                     DKIGLD"
     gene            complement(7956..8564)
                     /locus_tag="PC407_002864"
     CDS             complement(7956..8564)
                     /locus_tag="PC407_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF2498291.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         2887 a         1445 c         1250 g         3155 t
ORIGIN      
        1 ttctacattt tttataaatt ttattcattt ttatattttt atgtatataa attctttttt
       61 gtaagtttat taatttctaa ttatcttctt taaatacatt gaaactacat cagaaaatct
      121 agttatatct cttgaatata taaaatcatt tccatatatt aatctttcag cttcaagttc
      181 tgattctttt cctgtaaata ctcctagtac taatatattt tgctttctag cttttctcac
      241 ttcattggct gtatctctca aaccaactat acccctataa gccttttctc ctcttaatgt
      301 tctctcacta ctttttccta tctttacatc atttggtctt ccatcgctga gaactatcag
      361 gattttattt tcttcatctc tatttattag accactacat atagatttta ttgccaaacc
      421 atctctatta ttaccagcac aaaaatattc aaatatattt tcactatttt ttatactatc
      481 atcataatct ttaaatcttt ttaatattgt atagtctaaa aaactagaaa atcccatcac
      541 tctatttggt atgccaactt ctgttagagc atttgcaatt atgtaagctt gaattgccac
      601 atttccctga tttctacttt gagagccact tgcatccaat aatatatcaa taacgtattt
      661 ccctttttca ttttctatat ctttataaaa tattttatta ttattacttc taccaactct
      721 ccaagctcta tttgctaata tagtaccaca atcagaatat actctacttt tagcattttc
      781 ttctattaaa attcttgaca gactttcttt taatctcttt atttgctgtc tatacattct
      841 agcatgttct ctaaattttc ctaaattatt ttctttttgt cttgttacat attttagttg
      901 aaatacatta ttacactcac ttctaagaac tccatctgta aagtgtattc tacaaccttc
      961 atgaacattt ctacaatttt gtgtctcaat ttttttaatt tcacctttac tcaaataagt
     1021 ttttccataa taatgttcta tttttgaata tatcttattt gcagtgtctt catcaacata
     1081 tataactcgg tctgctgaat tgctacattt agaatcatca aaatccccta caccttctac
     1141 aagcatggat gaagatattg aatctatatc actttcaata atattttctt cttcatcact
     1201 ataaagttct tcatacatga aatcagcaaa tgtatcaaaa tttacttcta cactttctac
     1261 attattttca tcaataagac tattttcttc tccatttaat atgtattcga aataagttaa
     1321 atatataaca tctaatgctt ttaaaatctc catcatatct tgattggtat ctatatttct
     1381 tatatcatta actattcttc tagtcatact atctaatgct gggtgcttgt ccatattata
     1441 aagtacaaat gtgtatttaa ttttatccat aatattatca ttgtgtattt tggtaaaacg
     1501 tgaaacaata tctttataag catttcttct tatgtcttct atccctggcc tatctttaat
     1561 gaccttttct tcaactattg tatttaatat aacttgaatt agacccagta taatatcttt
     1621 gttgtatcct tttcttattc tatttgtaag atatttttta actatatccc aatctacata
     1681 ctttcttcta gcacctgcaa ttattgcaaa atatagacct atttcttttg aagagtaatc
     1741 tttttctgat atatctatac tttcattgta atcaccagct attgtccatg ataagttatt
     1801 agctctattt tcaaattcat aatcatcata tatccatttc ataatctcac ctatattaaa
     1861 tatgacttat tggaaataca tctatactct cccatttatt aggtattcta gttttaatta
     1921 catctccaac catttctttc tcatatacat caaaagtctt tcctgtaagt cccatattaa
     1981 ttgcaagagt tggttttaat cctcttctta tagtcttaag agatgccata agaccacgta
     2041 aatcaatagc tttactagat atctctccat tttgagactt caattgtaaa tctaaaaata
     2101 ttcctgcaaa gtgtattaat ttttcttcat cagcatctga aaattcattt tttaaaatca
     2161 tcattaactt atcttcttct ataggaggaa tatctattac cataaatcta gataccagtg
     2221 cttcattaag ttcttttgtt cctgcatatt cataattcat agtcccaata aatcttgttg
     2281 ctggatgtag attgactctc tcatacccag gtacatcaat tattcttcta tagtctaaag
     2341 ctgaatgtac cacaactata gcatcatttt ttgccatatt aatttcatca aatactccaa
     2401 aacctccatt aatagcacac tcataaactg agcctcttct aagtttaact tcattgtcta
     2461 taaatgtatc tgtacctata agtgttgaac tgtctgtatt tatatgaaat gatgtattcc
     2521 actgaggtct attaaatatt tcccctagat tatcagcaag taaatttttt cccgttgcct
     2581 ttggtcccga taataatata ttttcttctt ctaaaatagc tgctatacac atagacaaga
     2641 tatctttacc ataaaaataa gttttagact ttgttactct gtattctaaa tctttttcta
     2701 aaccataata atttctaaaa tacagtacat cttcaataag tttttcatct accccttgca
     2761 cccttagatt atctattata tccatatttt tacccctttt atataaatag tatttgtttc
     2821 atttcttatc accattttag cataaaagga tataagctaa aattgatttt ttaatctccc
     2881 ccattttaag taaaaccatg caccatttta tcactacaaa attaagagta ctacttactg
     2941 atatttcttg attttgttcg tctatacgta tataatatat gctgataaat tatgaggaaa
     3001 ggatgattga aatatggaat atatgtcttg cccagaagta gcaaaaaaat ggggcatttc
     3061 tgaaagaaga gtacaaaaac tatgtgaaga aagtcgcata ccaggagttt caaaactcgg
     3121 gtatatgtgg ctgataccaa agaacgcaga aaagcctgtt gatggtagag cgaaaagaag
     3181 aaaggaagta caatatgaat agcgttttga ttatagatga tgacaaagaa ctttgtgctc
     3241 ttatgaaaaa atgtgttgag caagaaaatt tatctgctat tactgcacat tgtggtgcag
     3301 aaggtctaca tttgctcgat aaaaatagac acacactctc tcttgtaatt ttagacataa
     3361 tgatgccaga tatagacggt tttcaagttc tgaaaaaaat tagagaaaca agtaatattc
     3421 cagtaattat gcttactgct aaaaatgacg aggatgataa agtttccgga ctacgtttag
     3481 gtgctgacga ttacctaaca aaaccgttca gcataaacga acttatggct cgtgtcaatt
     3541 ctcttattcg tcggtatact atgttaaacc ctgcttttat aacagacaca gattgcataa
     3601 taataaatgg catgattatt gacaagacaa accgaattgt ttctatcaat aatattcctg
     3661 ttcaacttac aagcaaagaa tttgatttac ttgcttttct ggcttctaat aagggacggg
     3721 tatttaccaa aaaacagatt tatacacaag tatgggaaga agaatatgct tttgacgata
     3781 gtaacatcat gtctttcatc agtaaattga gaaaaaagat tgaaccagac cccaaccatc
     3841 cattttatat tttgacagtt cgtggtgtcg gttatcgttt taataaggag gcttaacatg
     3901 aatacaagtg tctatctact tttgatattg gctcttagcc tgattattat agcgtacctt
     3961 gtttctgtcc tgctacgtgt ccgaatacaa cttacactta taaaagatgc tttagaggat
     4021 ataaaaaatg gaaaccttaa tcgccgtatt ttaacagatg aaaacgacat tacaaaacaa
     4081 atttgctatg atattaacga aatagcaatc aatagccaat ttcaacttat tcagcaaaaa
     4141 caatcagaac aagcctacaa acagctaatg acaagtctct cccatgatgt aaaaactcca
     4201 cttgcttctt tggtcggtta tttggaagca gtagaaagta aaattgttgt aggagaggaa
     4261 aaagacgagt atatccatgt tgcgtctaat aaggctcatt acttaaagaa ttttgtagaa
     4321 aatctatttg aatgggtaaa attggattct aaggaacaag tttttcattt tgatattttt
     4381 gacttgaatg aattatcccg taatattata tctgattgga tacctgtttt agaaagcagt
     4441 cattttgaat atgaatttga tataccagaa atagagtact tcttacgtat agacgcaaat
     4501 gcatataccc gtatcattaa taacctatta caaaatatta ttacccatag tagtggcaac
     4561 aaaatgacat tgagaatttt tgaaaacaaa gagcaagctc aaattataat aacagataat
     4621 gggaaaggta tatcttcaga caatctccca catatttttg aaaggttgta ccaatgcgac
     4681 cattcacgag cttcaaaggg aaatggttta ggcttggcga ttgcaaagga acttattaac
     4741 gctcataagg gaaatattac cgccaacagc actcccggta tgggaacaga atttacaata
     4801 ttgcttccta aagccctgta aaagtacgag gctttcttac tctaaaacaa gataaaagca
     4861 agattatggc aaggttttgg caaggttcat gttttataat tctaattgta gttgattttg
     4921 acgttgtatc aactatcttt tgtagaaaca aatttattaa aagtgaggaa attatatatg
     4981 aatgattttg tgattgaaac aaagcaactt acaaaaattt atgggaatca aacagtagtt
     5041 gacaaagtca atctccatgt aaaaaaaggc cgaatatatg gtctgttagg acgcaacgga
     5101 gctgggaaaa ccacaattat gaaaatgatt ttaggattga cttcaatcac gtatggcgag
     5161 gtagatgtat ttggagagaa tattaaggga tgtgaaaaac gagtatatcc ccgtatcggt
     5221 gcaattatcg aaacgccagg attttatcct aatctgacag gaacggagaa cttggagatt
     5281 tttgcaaaac ttcgtggtac agccgcccct aattctgtaa aaagagcatt ggagattgta
     5341 ggcttacctt ataaaaataa aaagctgttt agtaaatatt cacttggcat gaaacagcga
     5401 cttggtattg ccaacgctat cctgcatgac ccggaactgt tagttttaga tgagccaacc
     5461 aatggacttg accctattgg tattgcggag atgagaaagt ttattaggga cttgagcgtt
     5521 gagcatggaa agacaattct tatctccagt catattcttt cagaaatctc attgttggct
     5581 gatgatattg gaattatcga ccacggtgtt cttttggaag aaagtagtat gagtgaacta
     5641 gaaaagaaaa acagtaaata tatcctgcta caagtttcag atattccaaa gacatcactt
     5701 attttagagc gtcaatttaa cacgaaggat tattccgttc aagatgacca cactttacgc
     5761 ctttataaca ctactctgga tatgggtgaa atcaataaag cacttgtaat gcagaatgta
     5821 actgttatta gttctcaact ttgtaatgat actttagagg actactttaa aaaaatcaca
     5881 ggaggagagg gcattgctta aacttattca aacagaattt cttaaactgc gtcgtaggaa
     5941 acttatttgg ctgatgttgt tgtctgctct cattatgccg tttttcgcac tactatactt
     6001 caattatttt ggtaaaacag gtgttgagcc aacaaagttt tataaatggt ctgcatttgg
     6061 ttatacactt tggattattt tgcccgttgt tttgggtata ttttgcacca tgcttataca
     6121 cgaagaaaac cagtatgata tgctcaaaca gctttggatt gtacctatta gtaaaatggg
     6181 atactttttt agtaaatttt tcgttgtttt gatttactct atttgtttta tgctgattac
     6241 agccgttgct tctgtgttat ttagtgtact ttctggatat gtcgtattta gctgggcaag
     6301 cattttatat ttgttgaaaa aatgtttaga aattggtgta atcactgctt ttgtaatgtt
     6361 gccgattttg gcaatcgcta catctcaaaa aggatacatt ctcccaattt gtatcacatt
     6421 ggtatatgcc ttttccggtt ttgttataat gacaataaat atgtatttac acccattatc
     6481 cagtatggga gttattatca tgagaaataa agacattcca ggtcttatat tttcacagga
     6541 aattaatgtc ccattagcat ttctttgtat ttgtatttgg agtattgtat cagtattgtt
     6601 tgcaagcatt gcactaggaa gaagaaagtg aggtgtagat aatgtggaaa ttactttggg
     6661 ctgaatgtca aaaaattcgt cgttcaaaaa ttgtttggat tacatttttt gcatccatta
     6721 tgatagcagt catagttttt gcaggagggc aggatgtata taatgaccca gatttacatt
     6781 atggcttaaa gagcgtttat gacggttcac ggtatataga caatgcaggt tggtatatgg
     6841 acgcggtaca accttggtca actttttttg ttcttcctgc tgtcattgcc ctattaggaa
     6901 gctatattat ttgtcgtgaa gaagaggagg acactattaa atccctgcgt cttattccca
     6961 taaatgaaac aaaattaacc atagcaaaaa tgattattac ttttatattc agtatacttc
     7021 tttatttgtt actctttact ataacatttt tgactgaatc cgttttacat ttttccgatt
     7081 tatcgacaaa actggtttta agttgcatga aagagtattt tcttgatggc ataggtgtat
     7141 tctttgcaat ttctcccatt attgccttgg tatcgcgcat gaaaaaagga tattggcttg
     7201 cattagtatt cacagaagtt tattctattg ctggactatt tgcgggtatg tcaaatgtct
     7261 tacaaacctt ttttcctatt gcagcaatat ttaatatttc tggatactat ataactactg
     7321 gagaaaaaat tatggggagt atcataagtt tattactttg tgcctgcctt tctgcattta
     7381 tactgaaagg gctaaaacac aatgaaaaaa attgaaatag gtatatcaaa atgaaaaaga
     7441 taaaaaaact gattattcta attgtattct tgtttctttt tggcatgtta cactgttata
     7501 ctgaatggaa attcaacaat gacccaccta aaggggtaat agaaagaata aatgagtgga
     7561 ctgataagat tggactggat tagtaactta tgttaattat agtgaatgta atattagcaa
     7621 gttattgttt cgtattttaa tactataact ttaaatattt ggttttaatc ttccgaacga
     7681 atataaaaaa actgtcacag aggcaacata tttttgaata tttataaagt ggtagttttg
     7741 attttcaaag ctgccgcctt ttcttctata taagcaaact tatcctcaac aaagtgtaaa
     7801 acatggtaca ttttaagaaa aaagtaccac agacacaagc aataaaagaa acgccgagaa
     7861 cctacaaaaa cgtagtgctt atgcagattt acgacgcctt ctattttctc aaccgtctta
     7921 atgaccttgt acaaaagaaa tttttctaat attttttata atcttatctt gtgatgtttt
     7981 ttatgtataa atgacttaat aacaaatcca ataaaaatta tggatattgg tataattaga
     8041 acgaggaatt tataatcatt ataatgtgac attattctta cccaactctc agaaaaaata
     8101 tatcctatac tgattaatga agtattccat atggttattc ccacgataga tgatattaca
     8161 aaagttataa tattttgacc tgctatacct gctataaaag atatgtatgt tctacataaa
     8221 gggattaatc tacatacaca aacagaaata gaagagtatt tattaaaata atcttgagat
     8281 gcaaatattc ctttctttgc ctttggacat atctctatta ttttatttat gatagattta
     8341 cctccccaat ttcccactgt ataacaaaat atcgaaccaa gtatacctgc aattatactt
     8401 aaaattaaaa ttgtcagaaa tccaaaatga tttcttgcag caattgctcc acataaaggt
     8461 aaaacaactt cgctaggtaa tggaaaacaa gcatactcaa tcataattaa tacaaataca
     8521 gagattaatc catattgtaa aataaaacta ttgattacag acacttactt atctatctcc
     8581 tttcttattt aaaggtttta agggtacata ttctaaggat tctcacccta tatttaaaga
     8641 atcaatctct ttaaactatt atatgtattt acacaaagca tttataacat actttaaaaa
     8701 tagtacctta aaataagaaa cttaataaat aaaaaaa
//
LOCUS       DAMJVX010000142         8720 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMJVX010000142 DAMJVX010000000
VERSION     DAMJVX010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.147"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            6..770
                     /locus_tag="PC407_002865"
     CDS             6..770
                     /locus_tag="PC407_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HDF2498292.1"
                     /translation="MQIIDININNIDSRHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     AFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKGFCLADTSEPNYELLYFPFTDNAPIPCF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKI"
     misc_binding    993..1206
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1460..2494
                     /locus_tag="PC407_002866"
     CDS             1460..2494
                     /locus_tag="PC407_002866"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDF2498293.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKNI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEVL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2536..3753
                     /gene="argJ"
                     /locus_tag="PC407_002867"
     CDS             2536..3753
                     /gene="argJ"
                     /locus_tag="PC407_002867"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDF2498294.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKENDVLVASTGVIGKPLNINLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3768..4628
                     /gene="argB"
                     /locus_tag="PC407_002868"
     CDS             3768..4628
                     /gene="argB"
                     /locus_tag="PC407_002868"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDF2498295.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGMDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            4658..5845
                     /locus_tag="PC407_002869"
     CDS             4658..5845
                     /locus_tag="PC407_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDF2498296.1"
                     /translation="MNNNNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            5954..6976
                     /gene="argF"
                     /locus_tag="PC407_002870"
     CDS             5954..6976
                     /gene="argF"
                     /locus_tag="PC407_002870"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDF2498297.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAYVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQKIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7103..7720
                     /locus_tag="PC407_002871"
     CDS             7103..7720
                     /locus_tag="PC407_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HDF2498298.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     TVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(7940..8638)
                     /locus_tag="PC407_002872"
     CDS             complement(7940..8638)
                     /locus_tag="PC407_002872"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDF2498299.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
BASE COUNT         3501 a          861 c         1568 g         2790 t
ORIGIN      
        1 taagtatgca gattatagat ataaatataa ataatattga cagtagacat atatgctgtt
       61 caatttctga aaaaaaagga gaaaattgtg ttttttctaa aaaagcttgg atgaaaaata
      121 attttgataa aggtctagcc tttaaacgat ttgatgtaag aggtaaagct tttatagaat
      181 atattccagc agaaaatgca tggtgtccta ttactgcgga caattatata tttattaact
      241 gtttttggat atctggaaaa tttaagggtc aaggatattc taatctattg ctacaagagt
      301 gtgttgatga tgcaaaatca aaaggtaaat atggattagt tgtgctttcg tctagtaaaa
      361 aaatgccttt tttatctgat gctaaatatc taaaatataa gggcttttgt ttagcagata
      421 catcagaacc aaattatgag ctattatatt ttccctttac tgataatgct cctattccat
      481 gttttaagaa ttgtgtaaaa aaaagaaaat taaatgagaa cggtatagtt ttatattaca
      541 gtaatcagtg tccacataca gaaaaatatg ctcctatttt gaaaaaaatt gcaaattcta
      601 gaggtattga tgtaaatctt ataaaattcg aaacagcaaa agaagcacaa aatgctcctg
      661 caccatttac tacatacagt ctttttttta atggtgaatt tgtaaccaac gagatacttt
      721 ctgagaaaaa gattgagaaa tttttagata ccaacattaa aaaaatttaa ctaaaaatag
      781 atgtaaatat tgtattttga gattacatta ttttataatc caatatattt tggaaatttc
      841 ttaagttgat gaatgataat ataaagatta taactcaatt tagatacaaa atgctggaaa
      901 ttaaccatat agaaaatatt tccaaaaatt taaaaattat attgacaaag ttaaaaaaat
      961 aaactatcat ataaaacaat aagaaaataa attgaaatcc tgtgaaaaag agagtagcat
     1021 aaagctagtt gatagagagc tgggaatggt ggaagcctag taacgaattt tattgtgaat
     1081 agagctttgg aggagattac cagaagtaat agtatggtaa ttggtgaatc tcgccttaag
     1141 agattagagt gggtaagatt atcttatcaa ttagagtggt aacgcgggtg tatattcgtc
     1201 tcttggtctt tgaccaagag gcttttttaa ttttaaatgt acattttgta gatacttttt
     1261 aatttgagtt ttaatgatta ttgtaatttt accaatgaga gtggtaccgc ggaaaatcgc
     1321 ctcttagtat ttataatgct aaggggtttt ttgttttaat tagaatattt taatctacat
     1381 attatcaaat gattatcata tagtaaagtg gaaagataga attttaatac tatttgaata
     1441 taaaaatgga gggaacaaaa tgaataaaat aaaagttggt attattggcg caactggata
     1501 tgtcggggca gagttgatta gattattaat gaatcatgat aaagtcgaag tgacagcaat
     1561 aggttcaaat tcgtatgttg gaaaaaatat agttgatata tatccaagta taggatataa
     1621 aaataacatg atatgtatag aaaatgaaaa agttatagat atgtgtgatg ttgtgtttac
     1681 agcccttcca catggagtta gtgaaaagtt tgtaataaaa gcaattaaat cgaaaaagaa
     1741 agttatagat ttaggtgctg actttagaat aaaagatgaa gaagtatata gtaaatggta
     1801 tggagtatct tttatagata aaatcttaca taaaaaagct gtatatggat taagtgaaat
     1861 atacaaagaa gatataaaag atgcagatat aattgcaaat cctggatgtt atccaactag
     1921 tatttcttta ccattgatgc cattactaag ttctaaattg ataaaaaata ataatataat
     1981 aattgattct aaatcaggtc ttacaggtgc tggtagagaa ttatcgatta gtagtcactt
     2041 tacggaggtt aatgaaaata tcactgcata taaaataggg aaacacagac atactccaga
     2101 aatagaacaa aatttaagtg aatcctgcaa agaaaaagta agtgtagttt ttacaccaaa
     2161 cttaatacca gtaaataggg gaatcttatc aactatatat tgtacaaaag aagataatat
     2221 ttctataaat gatatacata gaaaattaac tgattattat gaatttaaag aatttataga
     2281 agttctccca ttagataagg tagcttcttt aaagaatgtt aggttctcaa ataaatgtgt
     2341 catatcatta catgaaaatg gagatacatt gataatctgt tcagctatag ataatatgat
     2401 taagggagca gcaggtcaag ctatacaaaa catgaatata atatttggta ttgaagaaaa
     2461 tacaggttta aaaaatattg caccatcatt ttagtaatga aaaaatacta aactcatata
     2521 attaggagga taaatatgga gattataaag ggtggagtta cagtttcaga aggatttttt
     2581 gcttcaggga ttcactgtgg cttgagaaaa aataaagaaa aaagagattt agctttggtt
     2641 tattcagatg ttttatgtga tgcagcagca gtagtaactc aaaataaagt aaagggcaat
     2701 cctgtttatg ttacacagga acacattaaa aatggaaaag cacaagctat catagtaaat
     2761 agtgctaatg ctaatacctt taatggaaaa gaaggactta taaatgctta taaaatggct
     2821 aaattcacta gtgataaatt aaatattaaa gaaaatgatg ttttggttgc atctacaggt
     2881 gtcataggaa agccattaaa tataaattta atagaagaaa atatggatga attagtgaat
     2941 aatctaagta aacaaggtca tattggagca agagaagcta ttatgacaac tgatattata
     3001 aaaaaagaaa tagcagtagc aataatgtat ggagataaaa aaataactat tggaggtatg
     3061 gcaaaaggtt ctggcatgat acatcctaat atggcaacaa cgttaggatt tataactaca
     3121 gatgcaaata ttgatggagt tttgctaaaa gaagctttga aaattgctgt tgataaaagt
     3181 tttaatagag ttagtgtaga tggagataca agtactaatg atatggtgat gattctagca
     3241 aatggaaaag caaaaaatga tagaataaac aaaaaagatg aacattatca agtatttttg
     3301 agtgcactta catatgtctg tatagaactt gctaaacttg ttgcaaaaga tggagaagga
     3361 tgtacaaaac taattgaatg ttatataaat ggagcattaa gtgaagaaca tgcagtaaaa
     3421 ttagcaaaaa cagttatatc ttcttcatta gtaaagacag ctgtatttgg agctgacgca
     3481 aattggggta gaatactgtg tgctttaggt tatgcaggtg aagaaataga tatggaaaaa
     3541 gttgatatta tttttgagag tatgaaagga tatattgaag tatgtaaaaa tggaaatgga
     3601 ttagatttta atgaggaaaa agcaaaaaaa atccttgaag atgacgaaat aagtatatta
     3661 gtagatttaa atatgggcaa tgccagagga aatgcatggg gatgtgattt aagctatgat
     3721 tatgtaagaa taaatggaag ttatagaaat taggaggata tactttaatg attaatatag
     3781 aaaaagcaaa tacacttata gaagctttac catatataga aaaacatcag ggaaagacta
     3841 ttgtagttaa gtatggtgga agtgcaatga aaaaagatgg tctaaaagaa tctgttatgg
     3901 aagaccttgt attaatgagt tatgttggaa tcaatatagt tttagtacat ggtggagggg
     3961 cagaaatcaa taagatgcta gcaaaagtgg atatagaaag taaatttgta aatggtctta
     4021 gatatacaga tgaagagacc atggaaattg taaaaatggt acttgctgga aaggttaata
     4081 aagacctagt aaataaaata catacaaaag gtggaaaagc agtagggctt tgtggaatgg
     4141 ataataatat gattttatgt gacccatata aaaattatga acttggattt gtaggagaaa
     4201 tcaaaaaagt aaatgttgag cttatagaat cttgtctaaa gtcaggttat atttcagtaa
     4261 ttgcaactat aggtgttgga gatgatggag agacttataa tataaatggt gacacagcag
     4321 cttctgctat agctaaagag ttaaatgcag ataaattgat acttcttaca gatgtccctg
     4381 gtttgttaag agagccagat gaagaaaaat cattaataac agaagttata ttagaggatg
     4441 ttgataaatt atttgaagaa ggtataatta ctggaggtat gatacctaaa atagagggat
     4501 gcgtagatgc cttaaataac ggagtaaata gggtacatat tttggatgga agagttccac
     4561 attctataat aacagagcta tttactgata gtggaatagg tacattaata agaaaagaaa
     4621 atgaataaaa ttataagttt aagaggggag aataagaatg aataacaaca atacaatttc
     4681 aaaatggaat gaatatttta tagatacata taaccaaccc aattttgtca tagattatgg
     4741 tgaaggaagt tgtttttttg atactaatgg caataaatac atagatttta caagtggata
     4801 tggagtatct tctcttgggt atagtaatag taatttaaag aatgctctaa aagagcaagt
     4861 agataaatta ttacatactt caaatttata ttttaatgag ccagtgttgt attcaggaga
     4921 gaaaataatt aattcaagtg gaatggctaa agtatatttt tgtaattctg gaacagaagc
     4981 aaatgaaaca gcattcaaaa tagcaagaaa gtatagtagt gacaaatatg gtaatggtag
     5041 aggaactatc ataagtttaa aagactcttt tcatggtaga acaatgatgt ctttaatggc
     5101 aactggtatg gacaaatacc ataagtattt ttatcctctt cccgaaggtt ttaaatatat
     5161 agaaagaaat aatattgaag atttaaaaaa taatcttgat tccacagttt gtgcaataat
     5221 tcttgaagcg attcaaggcg aaggtggagt taatgttctt gaaaaagatt atgttttgga
     5281 aatagtaaaa atatgtcaag aaaaggatat tgtagtgatt tttgatgaag tccaatgcgg
     5341 tataggaaga actggtaaac tttttggcta tgaatatttt gatgtaaaac cagatatagt
     5401 taccgtagca aaagggttgg gagcaggaat accagtagga ggagtattgg taaataaaaa
     5461 actttcaaaa gtactaggaa aaggagacca aggtacaact tttggaggta atttattagc
     5521 tatggtggca gcttcagttg tattagatga gatatctaaa gatggttttt ataatgaagt
     5581 attagaaaaa ggaaattata taagaaaatc tattgaaagt tttaataata aagttgtatt
     5641 aaagacaaaa ggtattggtc taatgatagg aattgaaact aatattgaat ctagtattat
     5701 agaagagaaa gctagaaaaa aaggattgct tattttgact gctggaaaaa atgtattgag
     5761 atttttacca ccattaacaa tcagttataa ggaaatagat gaggctcttg aaatcctaaa
     5821 agatatatta ctagagatta actaaatatt caaaaaatag ttaattaaag ataaaaatta
     5881 gaagtaaata aaaaagtaaa gtgctaacaa gtttaattga atttaagaag tttataagaa
     5941 ggggcgaatt aatatgaata atataagttt aaaaggtaag agttttttga cattaaagga
     6001 ttttacaaaa gaagagatta gatatttatt aactttatca agttatttaa aaactaagaa
     6061 aaaagttggc ataaaaggcg atttgctaca aggaaaaaat atagctttat tatttgagaa
     6121 aacatcaaca agaactagat gttcatttga agtagcagct catgatgaag gagcatatgt
     6181 aacttatcta ggtccaaatg attgtcatat gggtaaaaaa gagtctgtat cagatacagc
     6241 aaaagttcta ggaagattct atgatggaat agaatttaga ggctttaaac aagaaacagt
     6301 tgaaagtcta gcaaaatatt ctggtgtacc tgtttggaat ggacttacag atgaatatca
     6361 tccaacacaa atactggcag attttttaac agtaattgaa aatgtagata aagatttgaa
     6421 taaagttaaa tttgtatatg ttggtgatgc tagaaataat atgggaaact cactgatgat
     6481 aggatgtgct aaaatgggaa tacatttcgt agcactagca cctaaggaac tttggccaaa
     6541 tgaagaattg gttaaggaaa tgaaggaaat agccaagaaa tccaaaggag atataatgtt
     6601 gactgaaaat attgatgatg ttaaaggtgc agatgttata tatacagatg tatgggtttc
     6661 tatgggagaa gaggagcaat atgaagcaag aataaatcaa cttaaagatt atcaagtaaa
     6721 tatggatatg ataaagaaaa ctgaaaatga aaatgttata tttttacact gtcttccagc
     6781 atttcatgat ttagaaacta aggttgctca gaagatttat gaaaagttcg gattatcaga
     6841 attagaggtt acaaacgagg tatttaattc aaaatattca aaggtatttg aagaagctga
     6901 aaatagaatg catacaataa aagctgttat ggtttctact atgactgatg taaatatgaa
     6961 caataattta atatagatat gttttaatat agactttact ttaatgaaaa tttataaaat
     7021 attttaaata tattattcaa aaactctcat tatatgttaa aataacttat gtatcaacaa
     7081 attaagaggg aggatttgag atatgaaaaa aatcatatta gctttaacaa cttttttatt
     7141 agcaatatct ttaacagcat gttcaagtgc aaaaccagaa gatacaattg atagtttttt
     7201 taacagtgcg aaaaagtttg actttgaagg catgaataag gtaatggaaa ataatgacga
     7261 aaaatataag gatatattaa aggaattaga tacaaaagac ccaaatgccc aatatgtttt
     7321 agattatcta aaacagaatg catctaaaat cacgtataca ataaaagaca gcgaggttaa
     7381 aggtgataag gctacaatca aagtagaatg taaatttata gattcaacac cattacttca
     7441 agaaatagta gctgaggcat ttacaaagat gattggtatg agtttttcag gacaagactt
     7501 aactgatgag aaaacaactg aaatgttagt atcaattatg aaagaaaaac aaaaaagcgt
     7561 taaagagact tatgttacaa aaactgtaga gtttgaatgt tctaaaaagg ataataaatg
     7621 gataataagt tcagtaaatg atgcagtagc tgatgtattg ttatctaatc ttgttacagc
     7681 aggacaagaa ttttctaact ctatgaatac acttgaataa tattgtttag gtgtagtaaa
     7741 gtaataaata tatgattatt tttataaata aacatataaa tatttatgaa ggataccatt
     7801 tttggtatcc tttttattaa tataatgaaa tacatggtta atttctatat tgtcaagaaa
     7861 ctatgatata tgttataagt ctatactatt taaaataaac ttataacata tattttttat
     7921 aatatagtgc atgaaaaatt tatttaacta attttagata tttattgaca actgattcag
     7981 ccaagcattt tgatgaatca acaatattta ttgaaggata tgatttttct aaaactacat
     8041 ttaaatcagt acaagcaatt actatatttt ctatagattc atttttaaca tcttcgataa
     8101 gtttattcca aatatcaata ttgtgtggat tttctttatc catttttata ttttgaataa
     8161 gattgtttaa ttttatttgc cattcatctt taaatataaa ttcatgtcca ttatgtatta
     8221 taccattctg gtaaattgta gactcaaaag ttgaactagt cgaaaaaaga gttacttttt
     8281 gtgagatagt aggtaatttt ttaactgttt cttctacaat atttaatatt ggtatattaa
     8341 tagactcttt taattcttta aagtatatat gtgctgaatt gcatggcatt gcaataaaat
     8401 ttacaccagt tgattcaagc ttttgtaatc catcaattat agtttctttc ataagttcat
     8461 gatttattgg acggtcaata taaaatgggg taggtaatga atatatcatc atctttggaa
     8521 attcatcatc atattttgct ccatattgag attgacactc atcaataatt aaatcaacaa
     8581 aaggggctgt tgaccttgga cccattccag ccaaaatacc aattaatttt tcattcataa
     8641 aaattatctc ctttatatat aaatatattt atgtataaaa taaaaatcta taaaagcata
     8701 aaattatatt ttataataga
//
LOCUS       DAMJVX010000143         8706 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMJVX010000143 DAMJVX010000000
VERSION     DAMJVX010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8706
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.148"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(75..590)
                     /locus_tag="PC407_002873"
     CDS             complement(75..590)
                     /locus_tag="PC407_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDF2498300.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(953..2023)
                     /gene="leuB"
                     /locus_tag="PC407_002874"
     CDS             complement(953..2023)
                     /gene="leuB"
                     /locus_tag="PC407_002874"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDF2498301.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDEIGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTRAMGELIVNEI"
     gene            complement(2030..2521)
                     /gene="leuD"
                     /locus_tag="PC407_002875"
     CDS             complement(2030..2521)
                     /gene="leuD"
                     /locus_tag="PC407_002875"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDF2498302.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKEKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(2536..3813)
                     /gene="leuC"
                     /locus_tag="PC407_002876"
     CDS             complement(2536..3813)
                     /gene="leuC"
                     /locus_tag="PC407_002876"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDF2498303.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTITKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(3770..5512)
                     /gene="leuA"
                     /locus_tag="PC407_002877"
     CDS             complement(3770..5512)
                     /gene="leuA"
                     /locus_tag="PC407_002877"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDF2498304.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIKGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(5636..5882)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6030..6476)
                     /locus_tag="PC407_002878"
     CDS             complement(6030..6476)
                     /locus_tag="PC407_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498305.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(6497..7216)
                     /locus_tag="PC407_002879"
     CDS             complement(6497..7216)
                     /locus_tag="PC407_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2498306.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYSGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGTRVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(7334..8608)
                     /locus_tag="PC407_002880"
     CDS             complement(7334..8608)
                     /locus_tag="PC407_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDF2498307.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
BASE COUNT         2638 a         1718 c          884 g         3466 t
ORIGIN      
        1 tttttattgt atttttgaat tttatttttt ctaaatataa aatttatgtt ttaaaatata
       61 aaatttttat ttaatcaaag ttcaatatat tccacttttt ctttcgattc ttcaaaaaac
      121 cattcttcat tagaaaaatc accttctgca atttgtccaa taggcatttt catatacttt
      181 ccagttgctg tcactgctat atctccattt ggtagaatta tttctcctgt accttcaaat
      241 aattttctgt tttctttagt tattcttcca acaactttta tagtagcatc tgttggtact
      301 ggtttcttat atttcaactc taaagatact gttactcccc atatttggtc atcatttata
      361 cttacagctc ttccaatagt ctcatctaat attgcagcag acatacctcc atgaactctc
      421 ccaggataac tttgatgcca atcctttgta ttacaaattg atacaagctc tccattttca
      481 agttcataaa aatctgcttt aagtcctagc tcattttgtg ttccacaaac taaacaagat
      541 ttactactat tttgcttctt caaaacttta tactttgcca aaaaatccat ctcctaaccg
      601 acattagtag tcgcttaata ttaaataatt ctaaaatata ttatataata ttaataaaat
      661 ccctgcaaac ttctaataat aaaaataggc tacttcaata tgataagttt aatttaaagt
      721 attattttcc aaaaaataac tttatcaaaa tagaaactca aagtagatat aagcctcttt
      781 ttcgctatta aaatagtaaa aatatatttg acttaatata aaaaagagtg tctcattaac
      841 taataaagtt tattttgaga cacccctcat aaaacaaatt atactacaat agatttttac
      901 taattctaca agttatatat ttaaattata ttattcaaat taaactataa ttctaaattt
      961 catttactat taattcgccc attgctcttg ttcctacaac atttcccaca ccattataga
     1021 tatcaatagt tctataacct ttattgagta cactttgtac agcatcttca atacacttag
     1081 cttcttcttc taagttaaaa ctatgtctta acatcatgct tacagataga atagttgcaa
     1141 ttggatttac aatatctttt ccagctatat caggcgcact tccatgtatt ggttcataca
     1201 taccaaaact atcttctcta agtgatgctg atggtaacat acctatagaa cccgtaatca
     1261 tagatgcttc atcagatatt atatctccaa atatattatt agtcactatt acatcaaatt
     1321 ggctagggtc ttttacaact tgcatagcag cattatcaac atacatatgt gacagttcta
     1381 catcctcaaa ctctttagct aagtcttcca ttatacttct ccaaagtctt gaactctcaa
     1441 gcacatttgc cttatctaca cttataagtt ttttatttct atttctggct atttcaaaag
     1501 ctctcttacc aattcttctt atttcatttt cgttatatat ttctacatca taagcatttt
     1561 cgataccatc aatgattttt cttccacgtt ctccaaaata aatgccccct gttaattctc
     1621 ttacaacaac aaaatcaatg cctttctcaa ctatatccgt tctaagtggt gaagcttctt
     1681 ttatactttc atacattgta gctggtctta aatttgcaaa taaacccaat gactctctta
     1741 attttaaaag accagactct ggtcttttat ctcccttaca tttatcccac tttggtcctc
     1801 caacagcacc aagcaataca gcttcgtttt ttcgacaagt ttcaacagtt ttattaggta
     1861 gaggttctcc aatttcatca atcgcgcatc ctccagctaa tacatattca caatcgaatc
     1921 tatgattaaa tttacaacaa attttattta gaacctttat accttcatct ataatttccg
     1981 gtccaacacc atcacctttt atgactgcta tattacaatt catactccac taccccctat
     2041 ttgcttttat tgagttaatt aagccttcat ttaatataat attttgcata aactcaggaa
     2101 atggttctgc tttatattct ttttcttttg ttatattttt tatcacacct gtagaaaaat
     2161 ctacttctat attatcccct tcatctatgt tattggcagc ttcttcacat tcaagtatag
     2221 gaagccctat atttatagag tttctaaaaa atattctagc aaaagtagaa gcaatcacac
     2281 aggatactcc actttctttt attacaattg gtgcatgttc tcttgatgaa ccacaaccaa
     2341 agtttttagt tgcaactata atatctcctt tttttacctt agaaataaat ttatcatcaa
     2401 tatcctccat acaatgcgtt gctagttctt tataatcagc tatatttaaa tatctagctg
     2461 gtattattac atctgtatct atattgtcac cgaatttaaa aacacttcca ttggcaatca
     2521 taaattttac ctcccttaaa ttatttctga tggttttgat atttttccag ttatagctga
     2581 tgctgctgct acagctgggc ttgcaagata cacctctgaa tcaacatgcc ccattcttcc
     2641 tacgaaattc ctatttgttg ttgaaatgga tttttctcct gctgcaagta tacccatatg
     2701 tccccctaga catggaccac aagttggtgt agaaactgct gctcctgcct ctataaaagt
     2761 ggtaatatac cctttttcta tagcttcaag atatactttt tgagttccag gaaatatcat
     2821 aactctaact ccatctgcta cttttttgcc tttcattatc tcagctacta cttcaaggtc
     2881 tgatattctt ccatttgtac aagaacctat tacaacttgg tctatcttta tatccttatc
     2941 tatctcatct actgttctag tattttcagg aagatgagga aaggctactg tttcttttag
     3001 ttcggctaaa tctatttcgt atacttcatc atagactgca tcttcatctg cttcatatat
     3061 atttacatct tttgttatag ttgaacactt atgtgaattt atatattcca tagtcttatc
     3121 gtctactgga aagattccat tttttgctcc tgcttctatt gccatattac aaatagtaaa
     3181 tctatcatcc atagataagt attctactcc atctccacaa aattccatcg acttgtaaag
     3241 tgctccatca acacctatct cccctattat atgaagtatt atatctttac cacttatcca
     3301 tttatttggt ttattcttca aaacaaattt tattgctcca ggaaccttaa accaacattt
     3361 tcctgtagcc atacctgctc ccatatcagt tgaacctaca ccagtagaaa atgctcctaa
     3421 tgctccatat gtgcaagtat gagagtcagc acctataatt acatctccag cagttactat
     3481 acctttttca ggaagaagaa catgctctat tcccatatct cctacatcaa aaaaatttac
     3541 aactcctttt gattttgcga aacttcttgt aaatttacat tgttctgcac tctttatatc
     3601 tttattaggt gtaaagtggt ctaaaaccat cactacttta gtcttatcat aaacatcttc
     3661 aattcctaat ttttcaaatt ctctaatggc tactggtgat gttatatcat tacctaaaac
     3721 tacatctaaa tctgcttcga ttaattgccc tttcttaact tcatttaatt tagcatgttt
     3781 ggctaatatt ttctgcgtca tagtcatccc cattttttat ttcctcctca atataacttt
     3841 tatttattgc attcataagt gcattaatag ctgctgtatt tatgttttcc gatatactta
     3901 caccaaaata gacttcatta ttgtgctcta ttcctacata agctacccct cttgaatgtg
     3961 acccttcctc taatgcatgt tcccagtaat atctaaattt ataatctttc atgccacatt
     4021 gctttagtgc attattaaat gaatctacag gtccatttcc tataccaaca atcctttgtt
     4081 catttcccat ataacttatt cttgcagtca tctcaatcat gttagtatca ttagaatcat
     4141 cattttcata gtttaattca tccattgatt ttattttata cttcttaact ttataagggc
     4201 tctcaacttc aatatattct ttcttaaata aattaaatat tgcttcacta ctaagttctt
     4261 ccccaattct atcagattcc attttgacta catttccaaa atcagaatgc atattcttag
     4321 gtatcataaa tccataatct gtttccatta tataaacagc tcctcctttt ccagattggc
     4381 tatttatcct tataatttct ttgtattctc ttccaatatc atgagggtct attggtaaat
     4441 atggaacttc ccagtaatca ttatctctgt ttttcattgc tatcatgcct tttctaatag
     4501 catcttgatg tgaacctgaa aaagcacaat gtacaagatt acctgcatat ggatgtctat
     4561 catgtaccat aagttttgta cagtcttcat atataccaat tatcttatct atattgctaa
     4621 aatctagctc tgggtctatt ccttgtgtat aaagattaag tcccacattt actatatcca
     4681 tatttccagt tctttcacca tttccaaata gtgttccttc aagtctatcg gcaccagcca
     4741 ataatccaag ttcacttgca gccgtacaag ttcctctatc attgtgagta tgcaaactta
     4801 gtataataga atccctacaa cttatatttt tacaaaacca ttctatttgg tctgcatata
     4861 cgtttggtgt tgccatttcc actgttgatg gaagatttat tattactttt ttttgaggag
     4921 ttggtttcca aacatctatc actgcctcac atatttcaag tgcataatcc atctctgttc
     4981 ctgtaaaact ctctggtgaa tattcaaaag taaattctgt ttctggatat ttttttactt
     5041 cttcattaaa taattttgct ccttcaactg ctatatctat tatttcttgt ttactcatat
     5101 taaaaaccac atctctttga agaacagatg tagagttata taaatgtata attgcttttt
     5161 tacatccctt tattgattca aatgtttttt ctataagatg agccctagat tgtgtaagta
     5221 cttgtatagt tacatcatct ggaatcatat tctcatctat taattttctt aaaaatgtat
     5281 actctgtatt tgatgctgat ggaaatccta cctctatttc tttaaatcct gtatcaataa
     5341 gcattttgaa cattctgact ttttcattta cactcatagg cgttggcagt gactgatttc
     5401 cgtctcttaa atcgacacta caccaaattg gagccttaaa tatttcatta tctggccatt
     5461 tacgatttac tatttgcatt ttatcatatt ttttatactt tccacacttc atgttatttc
     5521 cccctttttc tgttttttgt attcagtata ccttatatcg acgcaatcct gcacacttca
     5581 taaggataaa tgtcaatttt atcattagaa taaaaaaaac ctcatctcta ataaaattag
     5641 agacgaggtt ttgctcgcgg taccactcta tttagctttc taaaaagctc ccttagtaat
     5701 agccaataca ataattataa tgactatatt tcctataacg tggaattact ccgattattc
     5761 taagatttaa atcttagaaa acagctccta gacaagttca gaactatcaa ctactaaatc
     5821 acaccatcct ttagctctct gaaagtttca ttgttcttac tacttctatt catcactttt
     5881 ttaatcattt aaattttcta aatattttga tttaatagtt attatataca cattaaaatg
     5941 tattgtcaac aaaaaaaata attatctcaa ttttttattt ttgataagca attctataaa
     6001 acatgctaac tattgaaaac agtatgatat tatgccttaa actttttcat cttttcatct
     6061 atgatattca ataatttttt ttcatcttcc aaactcaatg cccttaatct aattttataa
     6121 ttagattcgt cagtgtatat atttaatata ttttttacta cttctatttt ttctattatc
     6181 ttataattaa acttttgact ttgtttagat tttatactga atatacattg attatttatt
     6241 aatattgaat gattatcgag agtaatatat acttttccct catatttatt tgaaaaatca
     6301 cgaccttgtt taaatagaaa aactgctgtt attatacata aaatatataa tattgtcgtt
     6361 gaatatataa aaattgtagc taaaattata agtgctaaaa ccatatatat agtcattcct
     6421 tttttaaaat aaccattgtc ataataattc atatagataa acttattttt actcaaattt
     6481 tttcactccc aagattctaa aaatttatat caaaaaattc tataatttca tcttcatgat
     6541 aatttatgac taattcatct ggaaaatcta ctgcttctaa aactttttct gcattttcaa
     6601 attctcctat agaataacat atatgagagt cagtaccaag aattactctt gtaccatatg
     6661 ttttacacaa tgtcaacata tgacttacat tttcccaagt acccgttcta tgagagtttg
     6721 aacttaatga tgaattattt atttctaaaa ggatattttt atctttagct ttttttacta
     6781 taggttcata atctaaagga tacctactgt catctggatg tccaattatt tttaccttag
     6841 gtttatccat aacttttaaa atagcatttg tattttcttc ttttgtacca gaagctatac
     6901 aaggtctatg caaacttgcg attatataat ccaaatgttg caacatatct tcttgaacat
     6961 ctaaattacc actataatcc attatatttc cttctattcc cctaagaatc tttacacctt
     7021 gcacttcacg tggaagcaaa tgcaaattat aaaaatagaa tggatgtggt cctcctggca
     7081 tattaggtcc gtggtctgat attcctaagt attttatatt cttcttactc gcctcttcga
     7141 cattttcctt gaaagtacta tgtgcatgtc cacttattag agtgtgacaa tgtacatcca
     7201 taatagactt tttcattaaa tttcctccta atattttaca taaattcgtg aaaatagcta
     7261 aagggctatt tcaaaactaa agctgtcata atagacagtg taacgtttta aaacagcccc
     7321 attttcaatt attttacact tctaaatctt taaatttatc tttaaataat tctccaatac
     7381 ttcctaaaga ttcctctact tctaggtatt ctgttatatc ctcttctggc tctctagttg
     7441 cttcttttat tgaaaccaac attcttttag tctttggttt aaaatcaaga atcttaactt
     7501 taacctcatc tccaatatta acaacattag taactttagc cactctagta tcactcaatt
     7561 cagatattgg taaatacgca tctacacctt ctttaatttg aacaaaagcc cctttttcta
     7621 ttattctaag aactttagca ttgacaacat catcaatttg tacttctttt gctattaaat
     7681 cccaagggtc attatttatg tcttttaatg ccaaggcaat ttttttagtc tctttattta
     7741 catccaacac ataaacttct acttcttgtc cctctgaaac aacatcttca acactttcaa
     7801 ctcttgtcca agctaaatta tttatatgtg ctaatccttc aatcccacca atatccacaa
     7861 atgcaccata aggcattatc tttgtcacct taccatctct tttttgtcca acttcaagag
     7921 aattaaataa gtcttcttta gctgctttga tacgagcttt ttcttcttct ctttcttgct
     7981 taattcttgc tctttcagct tctttttctg ccttaatttg agctcttctt tcttctctct
     8041 ctttatttat acgctcttgt aatacatctc tatgagatgc tacaagtcta ttttttctaa
     8101 catccaattc ttttatataa acttctaagt taagaccaac aaatttagat gtatctgtaa
     8161 taaattttgt atcaagttga gaaattggta taaaaaatgt atatgtattg taattagcaa
     8221 atacaccttt atctatattc ttttcaacat ttacagttat tattctatgt tcatcataag
     8281 ccttttgtaa ctcggcaaaa tcatttcttt tgtcaagttg taatttagat aaagttattg
     8341 taccatcttt ttgagatact tttgttatta cagcagttat ttcatcacct atgtggtata
     8401 tatcttctat atattgattt ttttctatgt tcaattcact tataggtatt attccatcaa
     8461 acccatagtc taatcctaat tgtacttcat catttctcac tgcagttatt tttccagttg
     8521 tcaattctcc aactttcact ttgctgaata cttgttcttg ctggtcaagt aattcttgca
     8581 ttgttaaatc attttccatt ataaacatcc tttcctctta ttgattatca tatctgtaaa
     8641 tattatacca ttaatattgc aaatacttac atttttctta ctataatttt taaaataaat
     8701 tcaact
//
LOCUS       DAMJVX010000144         8566 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMJVX010000144 DAMJVX010000000
VERSION     DAMJVX010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8566
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.149"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(12..908)
                     /locus_tag="PC407_002881"
     CDS             complement(12..908)
                     /locus_tag="PC407_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2498308.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDFLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            1050..2477
                     /locus_tag="PC407_002882"
     CDS             1050..2477
                     /locus_tag="PC407_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2498309.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(2674..5268)
                     /gene="clpB"
                     /locus_tag="PC407_002883"
     CDS             complement(2674..5268)
                     /gene="clpB"
                     /locus_tag="PC407_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDF2498310.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSDDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5305..5901)
                     /locus_tag="PC407_002884"
     CDS             complement(5305..5901)
                     /locus_tag="PC407_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498311.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(5918..6322)
                     /locus_tag="PC407_002885"
     CDS             complement(5918..6322)
                     /locus_tag="PC407_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDF2498312.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNICFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            6689..7063
                     /locus_tag="PC407_002886"
     CDS             6689..7063
                     /locus_tag="PC407_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2498313.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            7060..7923
                     /locus_tag="PC407_002887"
     CDS             7060..7923
                     /locus_tag="PC407_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498314.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRNPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            7923..8561
                     /locus_tag="PC407_002888"
     CDS             7923..8561
                     /locus_tag="PC407_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2498315.1"
                     /translation="MKGLILKDLLNLKGNVKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT         2868 a         1269 c         1007 g         3422 t
ORIGIN      
        1 atatttatga attatttcat atcttctttt aaaataacat tactaacttc tctatgttca
       61 tttctatact tagttggaga tatattataa ttccttttaa ataagcgact aaaataattt
      121 atgctatcaa agccacatct catagcaatt tctgtcatat ttaaatctgt ttcctttaaa
      181 taatatatag ctttatcaag cctgattcca tttataaaat cgattgtagt cttacctgtt
      241 atttgcttaa atgttctaca aaagtgatgt gcactcaagt tcacacgact agcaagttct
      301 tttaatgtaa ttttatccat atatttttct tctatcaagt caaagataca ttcaaatttt
      361 tctaaagaat actttctttt tataaattca tcttcagaat atattttatc aatatgactt
      421 cttaataaga gtactattaa ttgatagata ttagctttta tagataactc atatccaact
      481 tctttattgt gatattcatc aagtatagtt gtaatacatt ttaaaatcag aaaatcattt
      541 tgaattaaat tattaaatac aatatgattt aaagccaatg gtgctaagta cttagtttgt
      601 aacaaatcaa cttgattact aaaaaggaat gtgggctcaa tacgaatact atagaatttt
      661 aaattatctg ataagctttc taaataatgt aattcattac tgtttgctat tacaatattt
      721 ccaggcacta ccttaaaatt atccaatcca cattcaagat atgctatacc atcaataaaa
      781 taatatattt gtagatgctc atgccaatgt cttttaaagt ttgctccttt tttaatgtca
      841 tcctttaaaa aaatatctat aaagttttct tgacctttcc tattaaaata tgtattttta
      901 tccttcatta ttcccccctc atcaaaatag tgctaattat aagcaatata gttattgtat
      961 tcattatttc atatcgctat tatataaaat gtaactatca ttgtaaacat taacataatg
     1021 ccagtcatta taataaggag gtaataaaaa tgacaaactc ttttaaaaaa gatgataata
     1081 gaaaaaaaat cacagtttta tttgtagtca ttgccatgac ttttatggca acattagatt
     1141 cgagtattat aaatgttgct ctcccagttc ttgcaagcaa actaaatgta tctctagcat
     1201 ctattgaatg ggtaatagca agttactcaa ttattatttg ttcaacacta ttattttttg
     1261 gaagacttgg agatattatt ggtaaatcaa gagtattcca agtaggtact attctcttta
     1321 catcagcttc attactatgt ggattaagta attctttaac tttacttata gtttgccgct
     1381 ttattcaagg aataggcgct tctgcctaca tggcaaacaa tcatggaatt atcacagaac
     1441 tatttccaaa agaaagcaga ggaaaagccc tagggattct agttactgca gttgctattg
     1501 gaaatatggt tggaccatct gtgggaggat ttattttgtc aatatttgat tggaatgtta
     1561 tattttttat aaatattcca atcggtctta ttgtaatctt tttaaataca aaatttcttc
     1621 ctaatagtaa aaaatcttca gaaaatatgg ataaaactgg tgctatactt caatttttag
     1681 gaacaacatt attttttagt gctctaatta gtgctcaaca aacaggatta ctaaatccat
     1741 atatattaat tgcactatta ttatcaatta tttttattat attatttttg atattagaaa
     1801 aaaaacatcc tcaaccgctt cttgatcttg aaatttttag aaattttaaa ttttcattga
     1861 atttaatttg tgctcttaca tcattcattt gtattgcatc atcaagtatt ttaattccat
     1921 tttacttaca aagtactatg aaacttcccc ctattcaagc aggtttattt atgatactat
     1981 cacctttgat tttagccatt ttttcaccta tatttgggaa tatatcagat aaaatcaagt
     2041 cagaaaaaat tattttaatt ggtcttctcg ttatgagttt tggattcttt ctaatgtcaa
     2101 gattgaaaga aagttctgcc cttattttat ttgtaattta tatatcgata atatcaatag
     2161 gacaagctat atttcaacct gctaacaatg ctttgataat gtcaagctgt tctagaagta
     2221 agctaggagt tgtaggaagc ataaattccc ttgtaagaaa tttaggacaa gttataggta
     2281 ttactatttc tacaacactt ttatataatt ttatgagtat taaagctgga tatagggtca
     2341 atgattatgt gattaataat gataaaatat ttgtatttgg aatgagaaat gtttatatta
     2401 ttgttacact tgtttgctta ataggtgcaa tattaattgg cttttattta tttaaatata
     2461 ataaaaatga acagtagttt tttataatta tattcaaatt atgattatat tataagaaac
     2521 ttactaaaat acttcctttg agttaaattt aatttggaca tattgactta aaaatgtttt
     2581 attaatttga taaaatattt cttttcctat agtttataaa taaaaatcag ctgagcaaaa
     2641 tatttagtat attatgctca actgattctt ttattattta actaaaactt caatattttc
     2701 atcttttcca tctataacta tagtacatcc attatacaca tctccagcaa taagtttctt
     2761 tgcaattata gtttctaatg tatttcctat atatctctta agaggtcttg ctccatatac
     2821 agggtcatat ccttctctca ccataacatc ttttgctgaa tttgtaactt caatagtaat
     2881 atctttttct tttaatctat tttttaaatc tttcataaat atatctataa ttttcttgat
     2941 attttcttgg tctaaaggct taaacattat aatatcatct actctgttta aaaactcagg
     3001 tttaaatcta tgcttcattt cattcattac taaattgtta gtagtctctg ttatatttcc
     3061 tcctgcttct aatagataat tacttcctat attagaagtc attattataa gagtattttt
     3121 aaagtctact gtcttacctt tattatcagt aagtctacca tcatctaata tctgtaagaa
     3181 catgttaaat acatcctcat gtgctttttc tatctcatca aatagtatta cagaataagg
     3241 agcacgtcta actgcttctg taagttgacc accctcttca tatcccacgt accctggagg
     3301 aggtccaact agtcttgata cagcatgttt ttccatgtat tcagacatat ctattcttat
     3361 tatattatcg tcactatcga ataaatttct agcaagagtt tttgcaagtt cagttttacc
     3421 aacacctgta gggcctaaga aaataaatga accaattggt tttctttcat cttttaatcc
     3481 tgctcttgca cgtattactg catttgaaac tgctgtaact gcttcatctt gtccaataac
     3541 tcttttatga agttcatctt ctagtttaag taatttttct ctttctcctt caactagttt
     3601 agttactggt atacctgtcc actttgatac aatgctagat atttcttctt cagtaacttc
     3661 ttcttttaaa agagaatttt cagaaccatc attcatattt tcttcgtact gctttatttg
     3721 ttcttctaac ttaggtattt caccatattt aacctctgct gctttgttga aatcatattc
     3781 tctttcatac ttttcagctt tacctttagc ttcgtctaat tgagccttta agtttttaat
     3841 atctaatatt tgactctttt ctttttcata ttttgctgtc atttcatcat ttttagattt
     3901 taactctgct atttcttttt gaatatcatc caatcttgat ttgcttttat catcattttc
     3961 tttaagtaat gcttctcttt cagtttctaa tgtaaataac tttcttctaa caacatctaa
     4021 ttctgttggc aatgaatcta tttcactacg aatcatagct ccagcttcat caattaaatc
     4081 aattgcttta tctggtaaaa atctatcttg tatatatcta tgagaaagtt ttgctgctgc
     4141 tacaatagca ttatcatgta ttcttactcc atgatgtatc tcaaatcttt cttttaaacc
     4201 tctaagtata gatattgtgt cttcaacagt aggttcttct gcaattacag gctgaaaacg
     4261 tctttcaaga gccttatctt tttctatata ctttctatat tcatcaaaag tagttgcacc
     4321 aatacagttc aattcacctc ttgcaagcat aggtttaata agattacctg catccattga
     4381 accttctgtt ttaccagctc caacgatagt atgaatctca tctataaaca gtataatctt
     4441 tccatcagaa ctttgaactt cttttaaaac tgcctttaat ctttcttcaa attcacctct
     4501 atattttgca ccagcaatta aagctcccat atcaagtgag aatacaatct tatcttttaa
     4561 accttctggg acatctcctc ttactattct ctctgctaac ccttctacaa ttgcagtttt
     4621 acctacacct ggctcaccaa tcaatacagg attatttttt gttcttcttg ataaaattct
     4681 tataatcctt cttatttctt catctctccc aattacaggg tctaatttat ttttcttggc
     4741 taaatctact aagtttgttc catatctagc aagtgcatca tatgtgcctt ctgggtcttg
     4801 tgtttctact ctttgactac ctctcacttg tgataaaata tttaaaaagt cattcttatt
     4861 tatattatat tgttttaata ttttaccaac agctgatttt gattctgttt ccatcattgc
     4921 aagcataaca tgctctacac ttatatatga atctttaaat tctttagaaa tactctctgc
     4981 ctttataagc acctcattta tttttctagt agctgatact ccttgtgagt cagcaccttc
     5041 tccatatact tttggaagct tatcaatttc aaagtttaca gtatttctca aagaatcaat
     5101 tgatatattc attttttcga ttatatttgg tatcaatcca tcctcttgat taataagtgc
     5161 tgagaaaaga tgaattacat ctacttgttg gttatgattt tttactgctt cgttataagc
     5221 ctcatttaaa cttttttgaa ctcttaaagt cattttttct acgtccatat aaatccctcc
     5281 attttaatat tatatccaaa taaattaaat tctctcattt ataattattg attttctaag
     5341 ctctaaaaga ctatcaagta aattctcgtt tttttgtata tcgaaactca atgttaaatt
     5401 aaatgttcca tctgaattaa aaaaatcgaa tacttctttt gattttgcta ttaaatacaa
     5461 gtcttcatct ataagtcttt tttcacctat taataaatac tgacatttat ctataaaact
     5521 ctttttaaaa tcaatagtgt atagattttc taaaatatta tattgataaa tagcaatatt
     5581 attaaacact tttaatatgt ttgaatttac aaaattgtaa atttgattat tgttatacag
     5641 tgatatttgt tcgtatatat attccctaaa taaaggatga atgttttgct taagttttaa
     5701 agtaacttca gctggtattt caaaataaag cttccagtac ttagagttat ttttttctaa
     5761 aataaaagct actttgttca aactggtaga actaatctcc tttagtgtac aattaaaatt
     5821 atagtgtctc aaactagttt tttcattctc aaatacatag tcaaaccact gtagaacatt
     5881 tctatcaagt tttaaactca tataaaaacc ttcttcctta tttactaacc ttattaggtc
     5941 tttttatctg attatattct gctaataaac tttctttatc agctctacta cctttatatg
     6001 ctatatgtgg atttatcata tactcaccat ttttcactct ttgtaaaaaa tccatttttt
     6061 tcaactccgt aaacaatgaa gataaagaag tttttggata ttcaaattca caagataatt
     6121 catataatgt ttttctaatc ttattatttt tatccataga gtttattaat tttaatggaa
     6181 ttaaatccct cttagtatac attccttcta ctactttaat ataattctta aaacaaatat
     6241 tcatatactt acttttattc ataactaaac ttttgctctc tgtatattcc tgatttaaat
     6301 ccttttttaa tgtcttatcc aaattaaata tctcttctca ctctccttat ctctaattga
     6361 taatataaat atattacaaa aaaatatgta tgtcaaaact gaaaatgcaa aaagtttacc
     6421 ttataagtaa acttttgttt agctataagc taaactttat tattttttta atttagtatt
     6481 tccaatttat tggcaggatt tttgaataat atttataaat tttttaaaaa aatttaaatc
     6541 tatttaatat ggcagccttt cataaaatac attacattta ttaaatgtat aatattggta
     6601 attagttttt tctaaaatca gttgacatat tatatatata tttatataat gtgtatatac
     6661 tatatataca atttaaggag gtgacttatt gaatatagtt attagtaatt cgagtgggaa
     6721 gccaatatat gaacagatta ctacacaaat taaaagtatg attataagcg gagagttacc
     6781 agaaggctca gctcttccaa gtatgagact attggcaaag gaacttagaa taagtgtaat
     6841 tactacaaaa cgagcatata gcgatttaga acgagacgga tttattgaga cagttactgg
     6901 taaaggcagt tttgtatcta gtaaaaacat ggattttgtc aaagaagaac aatttcgttt
     6961 aattgaagaa tacctacaaa aagcagttga tacagcaaaa agtagtgata ttacatatga
     7021 cgaattaaaa gatattttat ttttattata taaaggagaa tgataagtat ggatgcaata
     7081 actataaaaa atttaaataa aacttataaa gattttagcc tacaagatat atctttttca
     7141 gtaccaaaag ggagtgtaat gggatttgta ggtgaaaatg gtgcaggaaa aacaactaca
     7201 ttaaaagcaa ttttaaattt aatatcttat gatagtggaa atatagaaat atttggtctt
     7261 gatagtaaaa aaaatgaaaa agaaattaaa gagcaaattg gggttgtatt tgagggaagc
     7321 aactttcatg aaaatttaaa cactgaccat gtttcaaaaa tcatgagtaa aatctataaa
     7381 aattggaatg acacattatt taaagactac ttaaaaaaat tgagagtacc agacaataaa
     7441 ttaattaagg agttttcaaa ggggaataag atgaaactta gtattgctgt agccttatct
     7501 cataaaccaa aattattaat actagatgag gcaactagtt cacttgaccc tatcgttcgt
     7561 gaagaaatac tagatatatt tttagatttt atacaagatg aagaacactc tattatctta
     7621 tcttcacata ttacaagtga tttagataag atagcagact atattacatt tatacataaa
     7681 ggaaaaattg tatttagtga aaataaggat gaattaattg atactatggg agttttaaaa
     7741 tgtaaaccta gtgattttga taacctttca agagaggatt attcatatta tcgtaaaagc
     7801 caatttggat atgaagtact tcttaaagat aaacatagat ttatatcaag aaatccaaat
     7861 tgtattgttg ataatacgtc tatagaagaa attatgttat tttatgtgag aggtgataaa
     7921 taatgaaagg tttaatttta aaagatttac ttaatttgaa aggaaatgta aagtttatac
     7981 ttttattcat cattatgttt gggtttatgt cttctttagg agatggaaat gttaataact
     8041 ttataggtgt tattatagtt ttatgtacta caatgattgt ttcaacattt tcatatgatg
     8101 atttaaataa atgggatagt tatgtactta caatgcctat taatcgcaat gatattgtat
     8161 taagtaaata tttaactatg ttaatattta gctttatagg tgttcttgtt tcacttattg
     8221 ttagtgtaac tattggttat tttaaaaata cacttatatt aaatgaaaca ctacttatta
     8281 atgcactaat cttatcaatt tcagtatgtt ttggtagcct tattctacca cttatttata
     8341 aatttggtac agaaagagct cgtttattaa tgattttatg ctttttagtt ccaacactag
     8401 ctttattagt ttttaaaagt attttagaaa atattagttc acctatttct atagaaatta
     8461 tacttaatac actagtatat tctcttccat ttgttgctat tttactattt gtgatttcat
     8521 attttatatc atcaaaaatc tatagtaaaa aagaagtata aaatat
//
LOCUS       DAMJVX010000145         8564 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMJVX010000145 DAMJVX010000000
VERSION     DAMJVX010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8564
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.150"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            29..805
                     /locus_tag="PC407_002889"
     CDS             29..805
                     /locus_tag="PC407_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2498316.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGVGDLKELYRNINYHKLPSSSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(897..1649)
                     /gene="xth"
                     /locus_tag="PC407_002890"
     CDS             complement(897..1649)
                     /gene="xth"
                     /locus_tag="PC407_002890"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDF2498317.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(1810..2760)
                     /gene="galT"
                     /locus_tag="PC407_002891"
     CDS             complement(1810..2760)
                     /gene="galT"
                     /locus_tag="PC407_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDF2498318.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            3145..4341
                     /locus_tag="PC407_002892"
     CDS             3145..4341
                     /locus_tag="PC407_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2498319.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(4453..5799)
                     /locus_tag="PC407_002893"
     CDS             complement(4453..5799)
                     /locus_tag="PC407_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2498320.1"
                     /translation="MTMKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVV
                     EERQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKI
                     PLQFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALK
                     RVFPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDL
                     VPKIKDLVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKV
                     AKSNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAH
                     NKHQIAIVITENNGAINNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAY
                     EKLAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEK"
     gene            complement(5932..6684)
                     /locus_tag="PC407_002894"
     CDS             complement(5932..6684)
                     /locus_tag="PC407_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2498321.1"
                     /translation="MNLIDKLDINQKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            complement(6843..8420)
                     /locus_tag="PC407_002895"
     CDS             complement(6843..8420)
                     /locus_tag="PC407_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2498322.1"
                     /translation="MKQKMKEFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     IPFLENNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HTSVNYMLGCRGILAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFV
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFVSPILFVIHAFLSGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     ILGLVYALVYYNVFKVLILRFNLQTPGREEITDINFDLEPLSNKVEIAQSGETTKRAL
                     SIIKYLGGKENIEDITNCITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDKDIQIIY
                     GPSVSQVRDEVSFAMSI"
BASE COUNT         2985 a         1388 c         1021 g         3170 t
ORIGIN      
        1 taataaaaaa atttaggaga taaactttat ggaatacata attttcgatt tggaatttaa
       61 ccaaggattt gataaaaaac tcaataaaac tgtatccaat gaaaagtgtc cttttgaaat
      121 tatacaaata ggagcaatta aattggattc taaattcaat ataatagata cgtttaatag
      181 ctatgtaaaa cctacaattt ataaagacat acatcccttt attagtcgta tgactaatat
      241 taaaaattct gattttaatg attcgcctac tttccctgag gtctacaaca attttataaa
      301 atttatatca tcacaagacc ctatattatg tgtttgggga gttggagatt taaaggaatt
      361 atacaggaat atcaactatc acaagctacc atcaagttcc ttacctaaat cttatattaa
      421 tatacaacaa catgcatcta aatatttcaa taatccagca ggaaaaagca ttggcctaca
      481 aaatgctatt tctatattgg aacttgatga aaaaatgtct tatcacaatg ctttaaatga
      541 tgcatattat actgctaaag tttttattaa gatatacaat ccaagtatag tcccagatat
      601 atatctttat acgagtataa aacctaaaac tataagatat tctaataaaa aaagagttga
      661 ttatgataag ttatttgatg aatttaggaa aattttaaat cgtgaattaa ctaaagacga
      721 gaaaaaaatc attaacttag cttataatat ggggaaaaca aatcaattta cattagaaaa
      781 tgttaagcaa agaaaaaaca aatagagttt atttagataa aaaataggca tgtagattaa
      841 aaaactagtt aactttaaat ttatatctac agcccaattt ttatcgtaat taatttttat
      901 tttatctcta attcaactgg acaatggtct gaacctaata tttcagtgtg tatatccgca
      961 cttactaatc tatcttctaa ttttttggat gcacaaaaat aatctattct ccaccctgca
     1021 ttattttttc ttgcattaaa tctatatgac caccaactat aaacgccttc tttatcagga
     1081 ttaaaatatc tatatgtgtc tataaaccca ctgtctaata atttagaaaa cttctctctt
     1141 tcttctttag taaaacctgc atttttcata ttatttttag gattttttaa atctatttct
     1201 ttatgtgcaa cgttcatatc tccacacaca attacaggct tatgattatc tagtttggtt
     1261 aaataatcta taaagtcgtc ttcccatcta gttctatatt ctagtctctt taattcagat
     1321 tgagaatttg gcgtatatac tgtaacaaag taaaaatctt caaactctag agttatcact
     1381 ctaccctctt tgtcatgttc ttctatattt attccataca ttacttttaa aggctctttt
     1441 tttgtaaata tagcagtacc tgaataaccc tttctctcag cataattcca atattgaaaa
     1501 tatcctggta aatctaactc tatttgccct tcttgcaatt tagtttcttg taaacaaaat
     1561 atatctgcat ctacttcttt gaaaaagtct aaaaatcctt tcccaacaca agctcttatt
     1621 ccattaacat tccatgatat aaatttcata cttatactcc tcatctaaac attgacacac
     1681 tccccatagc taaagatggg ggcttttcaa caattttatt gtaaattctt aatttacttt
     1741 tattttaaca atactatact ataatttcat ctataattac tatttactta atacctaaaa
     1801 atatatgttt taatcaattt ttaacttttt tgttaaatct tcagggctca ttgaagaaac
     1861 atacatattt gttccaagtt caaatcctcc aaaattatat tttcttggga ttatgtgcat
     1921 atgaacatta aaatacgatt ttgtttcaat ctttgttgga tgagtgtgaa tacataagtt
     1981 aaatggcata tatccattga tattgtataa cttcttaaac aacttttcaa atattgtaga
     2041 aagctcctgt atattattat catttaactc accaaactct attttatctt taaatattat
     2101 tcttacttct ccactatact tggttgcata tggtataagt accaaaaaag cttctccatt
     2161 ataaacaact cttctatcta aacttatttc attttctatt aaatcatcat acaaagagct
     2221 tttattgctg tcataatact cctttgaaac taacagttca ttcgtaattt caggtggaat
     2281 aaaagacata gacaatattt gagcatgtgg atgcatcaaa gatgctcctg cctttctcaa
     2341 gaagtttttg aagatactaa catactctac tttatcatct ttcgacaaat ctctaaatct
     2401 atttctatac ataaaaaaaa catcttcaaa ttctttttga ctcatgttat aaaaactacg
     2461 attatgttta tcgctctcta tcattacttc atgaacacca tatacatctc ttgccatatc
     2521 atcaattata ggaaatttat tgtagacaga ttttacaagc catccttctt cactttctat
     2581 ttcaaataat gcatcagttg catgagcttc atttcctttg caaaatgggc attcttcatc
     2641 atattcatta gtttcttcat cttcatttgg gatatctgct gtatctaaag gtctttttaa
     2701 cctatctgta gcaaatataa caacatcatt agtaattgga tctattctta attctttcat
     2761 aattaacacc tcttagttaa tagcctttaa tataattata tgatatttat cgtatctgtt
     2821 aaataaattt ataatatttt ttataatttt gcatatttat tattatatat tttgtctata
     2881 gctgaaagat tataactaaa aaatcactat cagtgtatca gaatattatc ttactataaa
     2941 ttaatttatg aatacataat ttagtggcat aaacatgggt attttactat aatttgttaa
     3001 aagtttattt taaagctata ttaatttttt ttgatgattt ttacccataa gtatgttatt
     3061 attatcttgt aaagatgtct tgtattcaaa catacaattt acataataca atatacgtat
     3121 ttaaattaaa tagggggtat taaaatgaat tattcaaaca gagttagtgc aatgcaagcc
     3181 tctccaatca gaaaacttgt tccatttgca caagcagcaa aagataaagg aatcaaagta
     3241 tatcacttaa acataggaca accagacata aaaactccta aaggattttt tgatgctgtt
     3301 aaaaactttg atagcgaagt cttagaatat gctacatcag aaggtattcc agaattatta
     3361 gaagcacttc aaaattacta taaaacttac gatatgaatt ttgaaaaaga tgaattatta
     3421 gtaactaatg gtggtagtga agctttactt ttcactatga tggcagtttg tgacccagga
     3481 gataatctat tagttccaga accattctat acaaactaca atggatttgg tcaatctgta
     3541 aatgtagaag ttaatgctgt tacaacaaag gcagaaaacg gtttccacct tccatctaaa
     3601 gaagaaatat tgtctaaagt agatgataaa actaaggcta taattctttc taacccagga
     3661 aatccaactg gtgcaattta tacaaaagaa gaacttaata tcctagctga aatagctaaa
     3721 gaaaaagatt tatggataat agcagacgaa gtttatagag aatttgttta tgatgggctt
     3781 gagtatacaa gctgtggtaa cttagagggt gtacaggata gagttataat aatagacagt
     3841 gtttctaaga gatacagtgc ttgtggtgct agaattggtt ctatcgcttg caaaaataaa
     3901 ggtcttatcg ctcaaatact taaattatgt caaggaagac tttgtgttcc tacattagag
     3961 caaataggag ctgttgagtt atacaaaact cctgtttctt acttcaaaga agttaatgaa
     4021 gagtacaaaa aaagaagaga tgtattatac aatgaattaa tgaaggttga aggtgttata
     4081 tgtaaaaaac caactggtgc tttctatata gttgctaaat taccagttga aaatgcagaa
     4141 gatttcacaa tttggatgtt aaaagaattt aacaaagata atgaaacagt tatggtttgc
     4201 cctgctgaag gattctatgc tactccaggt ttaggacgtg atgaaataag attagcttac
     4261 atcttaaatg aaaaagacct tcatagagct gctacattat taaaagaagg tttagagcaa
     4321 tatgtagctt tacaaaaata attttttaaa cttaatttat ataaatttaa tgatataaaa
     4381 tgtccccttt tcagatatta tttgaaaagg ggacatttct taattaatat attaattaag
     4441 acttagacct gactattttt cattttcttt atatagtgtt ggccagtaat ctttattcac
     4501 tttaattaaa gcatctaaaa cttgtcttgc cttagttgga gagattatag ttctatttaa
     4561 tgttaaagat aaaatagctt tttcataaga ttgttcaaag tatgcatcaa ctgctaactt
     4621 ttcataagct agttgacctt ctaacagtcc cttataaaat gttggtatat tacctacatg
     4681 taaaggtctc ggtccattct ttgtaagaat cgcagctact tctaccattg catcatcagg
     4741 taagttgtta atagccccat tattttctgt tataactatg gctatttggt gtttgttatg
     4801 agctattgct tctgcaactt caactataaa ttccccatga gcatcattat gcactaaatt
     4861 agaacctata gatgttcctt tttctataac agatttacat tcttcaaata cacgcttttc
     4921 tcttccattc ataacttctc ttgctctagt acaatttata tttgatttag ctactttttt
     4981 atcaggatat agataatact gtacatatgt attaggtaag taatctggta catcttccaa
     5041 catatcttta accattccat aagtgtctaa ccaagattta tctctttgtt ctgcatcaac
     5101 aggttggaat ccagagtttg ctactaaatc ttttatctta ggtaccaaat cttcaccatt
     5161 tttatcatac aaatgtgtaa accatccaaa gtgatttaat ccaaagtata caggctcaaa
     5221 tgttcttgaa tccatgtcta acaatcttcc ataagacctt aacaagttaa ctggttggtc
     5281 acacatatta agtaatcttt tatcatctgg aaatactctt tttaatgctt ctgcaacaat
     5341 agcagcagga tttgtataat ttaatatcca tgctttagga gagtattttc ttacagtctt
     5401 aaccatttct atcatatcac ctatagacct taacccataa gcaaatccac cagctccaca
     5461 agtttcttga ccaaccagtc caaattgtag aggaatcttt tcatctaatt ctcgcatctt
     5521 aagaccacca gttctcattt gaacaaatac aaaatctaca tctataaatg cttcatctgg
     5581 gtttgtagta tattcaagtg ttgctttagg atattcttct ttaaataata tttttccaaa
     5641 ttcccctatt aatctttgac gttcttctac tacatctaac atagttactt tatttagagg
     5701 aaatctatca ctcattttac ataaagactt taaaagtcct ggagtccaag tactgcctcc
     5761 tccaactata gctatattgt atcttttttt cattgtcata ttaatcaccc ctgtttttta
     5821 ttaactaact tgtaagatga ttataatata taaagttttc tacatgaata cttttaggta
     5881 atcattaaaa atagttcaat tttagtattt ttttcaaaat tgtaattttt ttcatctatt
     5941 gtatttttca atgtatttat gaaacatata ttccataaga gaaagtgctg gcactctaga
     6001 cgtatagtta acactattaa aattatattg tggagaagat acaaataatt ttatatcagc
     6061 tatatttgat aatgaattga cacttatatt tgtcagagaa attactttaa cttgatttgt
     6121 ttttgctata ttacatgcct taataacttg agcagtctct ccagacatag atacagcaat
     6181 tacaaggtca tctttagtaa tatttgatgc agccaaatac atgagagttg aatcatcata
     6241 gtattttaca tttacaccta tagctgaaaa acgttgagac atttcagtac atactaatct
     6301 agaagaccca acaccaaaaa aatatatatg cttagaatta tgtatttcat ttaaacacat
     6361 atctatattc tctacattta ttaaattttg tgtatccata acatcagtaa aaatatcact
     6421 ttgactttta gtaaactcct tatttctttc atttctattt gaatatgaaa aaactaaaaa
     6481 gtttttaaat tcactatatc cagaaaagcc gagcttctga caaaacctta ctatgtatgc
     6541 agtcgaatta aaagtttcat ttgctatatc tttaatcttt ttttgattta ttttttcaga
     6601 attttttaga aaatagttta aaagagatat ttcatttgta gataattttt tttgatttat
     6661 atctaatttg tctattaggt tcatttatac ctccataata atgatttgta gttaaacaac
     6721 tttctcatat ttattatata aatatacact atacaacatt tatcttatgt ttattatact
     6781 acaaaaaaga caatacaaat tataaattag tattgccttt taatatttac attagataga
     6841 cattaaatag acattgcaaa agaaacttcg tctcttactt gagatacaga tggcccataa
     6901 attatttgga tatctttatc agatactttt ataacgccca ttgcacctgt atatttttta
     6961 aaagcatttt catctacttt actcatatct ttaactacta ctcttaagcg agttatacag
     7021 tttgttatat cttctatatt ctccttacca cctaaatatt taattatgga taatgctctc
     7081 ttagtagttt ctccactttg agctatttct actttatttg ataaaggttc taaatcaaaa
     7141 tttatatctg ttatttcttc tcgtcctgga gtttgtaagt taaatctaag tatcaaaacc
     7201 ttaaatacat tataataaac cagtgcatac actaaaccaa gtattataga ataaatccac
     7261 tttgtatttg gttgcataac tccaaatact actaaatcta ttatgccacc ttgagtattt
     7321 cctattgcta ctccaaatat atgcatcatc ataaatgata tacctgataa aaatgcatgt
     7381 ataacaaata atattggtga tacaaataag aatgaaaatt ccaatggttc cgtaattcct
     7441 gttacaaatg ttgctaaagc accagctagt aatatacctt taaccttcga cctattttca
     7501 ggctttgcag ttttatacat ggcatatgct gcagcaggaa gaccaaatac cattataggt
     7561 attttccctt gagctaaaaa tctagttgct tctgttgaaa aactgattgt ttcagggtca
     7621 cttagttgag ctaaaaatat gttcaatgct ccaaatactt cttgtcctcc tacaactaaa
     7681 gaacctccta cttcagtaaa tctaaacata gcatttaata tatgatgtaa tccaaatggt
     7741 attaaaagcc tttcaagaaa tccaaataca aaggttccta aatatcctaa actagctata
     7801 ccaataccta cactctgaat aattgtattt acatatggcc atatatatgg tattattatc
     7861 cccaaaatag agaatactac tgttgttatt attggaacaa acctagttcc accaaagaac
     7921 cctaaataat ctggcagttt tatattatag tatttattat gtaatgatgc tgttattata
     7981 ccaactacaa ttccaccaaa tactcctaac tctaatgttt gtatgcctaa agttacagct
     8041 tgacccattt ctctcatcat gtcaggttca gcaagaatac ctctacaccc taacatataa
     8101 tttacactag tgtgtaatac taaataacca acaaaaccag aaaatgcaga aactgctttt
     8161 tcttctttag caagaccaac agatattgat atagcaaaga atattgctaa gtttccaaaa
     8221 agtactcctg ttatagcttt tgtaaaaccc aatattcctt gaataaaatt attttctaaa
     8281 aaaggtatat cactaactat attaggattt gaaaatgctg aacttattcc taaaaatata
     8341 cctgcagcag atattaatgc tattggcaat aaaaatgatt ttcctaactg taaaaaaaac
     8401 tctttcattt tttgtttcat ttctatccct ccatttttaa atttttaaaa cgttttctat
     8461 gtcataatta tatattttta ttttgttttt gaaactttat aatttgtttt aaaatatttt
     8521 tacataaata gtaatttttt caaaactgta aattttttca aaca
//
LOCUS       DAMJVX010000146         8502 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMJVX010000146 DAMJVX010000000
VERSION     DAMJVX010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8502
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.151"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            16..2193
                     /locus_tag="PC407_002896"
     CDS             16..2193
                     /locus_tag="PC407_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2498323.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALHSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANVMNIDDDLLKAGYEASIRHMFKKTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDNEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYRESDINSIIDDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2207..2629
                     /locus_tag="PC407_002897"
     CDS             2207..2629
                     /locus_tag="PC407_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2498324.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGACPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            3036..3839
                     /locus_tag="PC407_002898"
     CDS             3036..3839
                     /locus_tag="PC407_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2498325.1"
                     /translation="MKKLKLIIMLALVLALLITGCSSGNNKNKEEQKTIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMVEPPIQFGAC
                     SFAIQKNEKGEKWTKEINEVIEEMHKDGTLTKISEAWLGKDITKEPINE"
     gene            3832..4500
                     /locus_tag="PC407_002899"
     CDS             3832..4500
                     /locus_tag="PC407_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2498326.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMTEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     GKAY"
     gene            4736..5398
                     /locus_tag="PC407_002900"
     CDS             4736..5398
                     /locus_tag="PC407_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2498327.1"
                     /translation="MIFNMKYFLELFPIIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMNDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDKGQIEAAYVMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            5458..6255
                     /locus_tag="PC407_002901"
     CDS             5458..6255
                     /locus_tag="PC407_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2498328.1"
                     /translation="MVKRNIIKCLVAVLSLTFVVAGCSKKEEVKDEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVAASVNKD
                     NLFEEKVYGDGTNGLQDLDLGRVSAWIDAELTVVGDAKKANMKVRVLDEKLSVTNIAY
                     FFKKNDEKSKTIKEDVNKAIEEMLSDGTVKKISEKWFGMDVSADIQK"
     gene            6388..7563
                     /locus_tag="PC407_002902"
     CDS             6388..7563
                     /locus_tag="PC407_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2498329.1"
                     /translation="MIETKIKSIALKNREKIIKIRRQIHSNPELAFKEFKTSKLIKEE
                     LNKLKIEYIDVAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSINDNMHA
                     CGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGVDALAT
                     GHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAVYSSIQ
                     QIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKILENIV
                     RNVCRAYGADCKFEKSTGGDAVINDKNMIELGRESACKILGVENVEIIDFPAMTGEDF
                     AIYMKEKPGLFMYIGVGNKEKNINYRLHSNKFNIDEKCLSIASSLFSQLAVDYLCQL"
     gene            7672..8421
                     /locus_tag="PC407_002903"
     CDS             7672..8421
                     /locus_tag="PC407_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2498330.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEINQSEVVAILGPSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDIANLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSNKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIVRKEEVFS"
BASE COUNT         3371 a          863 c         1585 g         2683 t
ORIGIN      
        1 atttgaataa aaagaatgta taataagtat aataatttgt atttattatt gggaaaggaa
       61 aatgttaata tggatagtta tagacaactt aaagctaaaa taccctgtaa ggaaacaggt
      121 atagaaataa agaggacttt atgtgatatt tgttctccag gagtacattg tggaattgat
      181 gcatatgtta aagatggtaa agtaataaaa atagaaggta caaaagaaca tccaatgaat
      241 aaagggttgt tatgtaccaa aggtctttgc aatagagaat atatatatag agaagataga
      301 ataaaaacac ctctaaaaag aattggaaaa agaggtgaag gaaaatttaa accaatatca
      361 tgggatgaag cttatagaga aatagcagaa aaattaaata gagtaaaatc tgagtatgga
      421 gcaaagcatg tagctttttt tacgggttat ggaaagtggt atagaccttt tttgcaaagg
      481 tttgcatctt catttggttc acctaattat ggtacagaat ctagttcatg ttcaacatca
      541 gcacagatgg ctggaaatgt tgctactgga agaaacttta caatgcaaga tatggagaat
      601 gcaaaaactt atttagggtg gggatttaat ccatactact ctaaattttt aaatagtgtg
      661 aatctaataa aattaaaaga aaaaggtcta aaatttataa ttgtagaccc tagaataaca
      721 ccgacaactc aaaaactagc agatttacat ttaagaatta aacctggaac tgatggagct
      781 ttagctcttg gaatggcgaa tctatttata caaaaggatt taatagatag agaatacata
      841 gaaaagcatg tacatggatt taatgaatat aaagaatatg taaaattatt tgatttagat
      901 actgtaagta gaattacagg agtttctaaa gataatatag taaaagctgt tgagatgttg
      961 tcacaaggtc cattttgtat aaatgaaagt gcagctccaa tagttcatca tcaaaatgga
     1021 tttcaaaact atagagcaat gatgtcttta tctgctataa caggtaatta tgatagaata
     1081 ggtggaaaca taccaaaaca agattacttt gctggtaaga taggtggatt ccaaacacta
     1141 gaaaaagagt ttgtaaaaga agttctacct aaaaatataa atgaaagaat tggtggagaa
     1201 agatttcctc tatggaatga aatggtaact caatttcaag ctatggattt acctaggcaa
     1261 atagaagaaa agacaccata tgaaataaga gctttgtttt gtcatgggat aaatgctaga
     1321 atgtttccta attcagggag actttttaaa gcgttgcata gtgtagattt ttttgtgaat
     1381 atagatttat ttatgacaga tacatcaaaa tatgcagata tacttttacc tgcgtgtaca
     1441 tcatttgaga gagaagaatg taagagttac ccaggagggt atattacata taccaaaaaa
     1501 gttattgata aattatacga ttcaaaatca gatgtagaaa tactttcaga tttagcaaat
     1561 gttatgaata tagatgatga tttattaaaa gcaggttatg aagcaagtat aagacatatg
     1621 tttaaaaaga cgtgtgtaga tgtagataaa cttaaagaaa gtgatttacc attaaaatgt
     1681 aaagatttta agccatatat agtaggctca tatacaagag aggggtatga tactagcact
     1741 tctaggtttg aattaaaatc tacaattatt gaaaaatata aagattttgg actggatgca
     1801 ctaccaacat atagaggatt taatgataat atagataatg aatatactca tttattgaca
     1861 tcaggagcaa gaatttcaaa tgcactacat tcaagacttc atgacatcaa actttcaaga
     1921 atattaaaaa atgaagctag ttgtgatatt tccagcttgg atgcaaaaga attgggtgtt
     1981 atcagtggag attatataaa aataactact aagataggaa gtttggatgt aaaagtcaat
     2041 gttactgata gagtcttata taagaatata catatgtatc atggatatag agaatctgat
     2101 ataaatagta ttattgatga taattttgac ccttatagtg gattccctgc gtatagaagt
     2161 tgcaagtgta agattgaaaa aatgaataga taaatggaga gatataatga aaagtttatt
     2221 taaatttaat aaagaattat gtacagcatg tggagcatgt ttgcttgcat gtatagacca
     2281 aaatgataat aatatagaca atggtgaact ttttagacat gtatttacaa tagaggaagg
     2341 tgaaaatata gaatattttt cattagcatg tatccattgt aatgagccaa aatgtttagg
     2401 tgcatgtcct aaaaattgtt ttaaaaaaga agaaggattc gttgtattag acaatcaaaa
     2461 ctgtataggg tgtaaacttt gtgaaaaagc ttgtgaatat ggagccttaa tttatggaaa
     2521 tgatggtaag gcaaataagt gtaatggttg tattgagaga tataagtgtg gtatggattc
     2581 accttgtgta atggtatgtc ctacaggagc attaaaattt aataaataaa ttaaatatag
     2641 agagtgactt atacttggta taagatattt ttacaaaaag ctatattgag tttgttataa
     2701 taaaaactca atatagcttt tttatggata tagttggata ctataatgtg atttttagta
     2761 atagtttttt gctattggac aaagactgta aaacatatta aacttatgtt gttcaaagtc
     2821 gaagtataat ataataacta gaaaattgat ttatagtgtt ataaaagcaa atttagtaag
     2881 gtataaaata agaggcaatc ttttactaca gtatcaatat ttgggagtta tgttatggca
     2941 gagccattaa acaattataa tggtacaagt gattcaacct ttgaaaatgt attatataaa
     3001 aaattaaaat aagattaaaa aggagagaat aaattatgaa aaaacttaaa ttaattatta
     3061 tgcttgcctt ggtattagca cttttaatta caggatgttc atcaggaaat aataaaaata
     3121 aagaagaaca aaaaactata agagttgcaa catcaggaac atattaccct tttgctttta
     3181 aagataaaga taaacttaaa ggatttgaag tagatttttg ggatgaattt gctaaacgaa
     3241 ctaattgtaa agttgaatgg gtattaactg attttagtgg attatttgga ttattagaag
     3301 ctgataaagc agatgttgta tctgctcaac ttactgcaac acctgaaaga gagaaaaaat
     3361 atgctttttc agatgcttat aattatagtg gaacaaacat tattgttaga gaagatgata
     3421 ctagtataaa atcaatagat gatttagttg gcaagaaagt aggtgtgggt actggagcag
     3481 tagcaaatga aatactaaaa gaaaagtacc caaatgatga aataaaaata gtaaattatt
     3541 caagtgcaac tcttaaaggg aattatcaag atctagaaat gggaagatta gatgcagtag
     3601 ttgcgcagga tgttgaggct ttaatagcta taaaagaaca aaaattaaat cttaaaatgg
     3661 tagaacctcc aattcaattc ggagcctgtt cttttgcgat tcaaaaaaat gaaaaaggtg
     3721 aaaaatggac aaaagaaatt aacgaagtaa ttgaagagat gcataaagat gggactttga
     3781 ctaaaatatc tgaagcctgg ctaggaaaag acattactaa ggagcctatt aatgagtaat
     3841 tttaattttg aattttgtct aagtatattt ccatttttga ttaaagcatt gggaataaca
     3901 atactaatat ctattgttgc atttttttta tcaatattat taggtgtaat acttgccttt
     3961 ttcacacaga caaaatcaaa agttttgcaa tacataacaa gagtgtatgt ttcattcttt
     4021 agaggaacac cacttttaat tcaaatattt ttcttttact ttggatttcc aatgatattt
     4081 tcttttatga aagattgttc accttattta gcaatagtaa tttgtttagg tgtaaattca
     4141 tctgcatata tgactgaaat tatacgagca tctattgaga gtgttgataa aggacaacag
     4201 gaagcatgtt atgcttttgg gttaagtaaa tatcagtcaa tggttaaaat aattctacct
     4261 caagcagcta catctgcagt acctcctatt gtaagctgta cactggacat aattaaaggt
     4321 tcttctttag gtctaactat aggaatacaa gatttaatgg gagtagctca agtagaggct
     4381 gcttcaagtt ttaaatattt tgagatatat atgataacga tattaattta ctgggcagta
     4441 tctataatat tgagtaatat acaaagcaaa gttgaaaaaa gaataggaaa ggcatattag
     4501 atttcaatat ttaaaaatgg gatgatgtag atatattatt acttgtaaat aaatttttaa
     4561 ttttagaaaa ataaacttag taatctaaat tattgagaaa atatagtatc aaaatggact
     4621 gtataatcaa gttaaaaaat agtttttcta ttgaaaaaat aaataaaatt ctattgtaat
     4681 ttaatattcg acaaagtttt ataaaaaaca acattaagct agggggactt attttatgat
     4741 ttttaatatg aaatattttt tagaattatt tccaataata atgaaatcgt tcaatgtaac
     4801 tgtaattttg gcaataagtt cattagtatt ttcattaatt ataggtacaa tagtagcatt
     4861 atcatcttat tacaaagtga aagtattgaa tcaaatatgt aaagtatatg tatctatctt
     4921 tagaggtacg cctcttatgc cacagttatt ctttttatat tttggactag cgtatatgaa
     4981 cgattttgtt aaaaatatgg acccagtatt tgcaacatct atagtactta gtttaaatat
     5041 gggagcttat atgtccgaaa cgataagggc atctatactt tcaattgata aaggtcagat
     5101 agaagcagca tatgtaatgg gattaacaaa tttacaaaca atgaaaagga tagtgatacc
     5161 acaggccgta agagttgcac tgccatcatt attcaataat tttatagatt taattaaagg
     5221 gtcttctgta gcttttgttg ttggagtaaa tgatataatg ggtgctgcca aatcacaagg
     5281 agcgttgagt tttaagttct ttgaagtgta tggagcagtt atggtaattt actggtgcat
     5341 aataactcct ttagggtggg ggcaaaaaaa attagaaaag aaaataagca attattaagt
     5401 tattaagaaa atacataaaa gtatttttat ataaaaaatg gtgtgaggag aagagatatg
     5461 gtaaaaagaa atattataaa atgtttagtg gcagttttat cgttaacatt tgtagttgct
     5521 ggttgttcaa aaaaagagga agtaaaggat gaaaaagtta taactgtagc ttcatctaat
     5581 tcagctgacc catattctta tgttgataat ggtgagcata agggttttga agttgatatg
     5641 tggaaagaga ttggtaaaag aagtggatat aagataaaaa tggaaccaac aggttttagt
     5701 tctatatttg ggatgttaga ttctggtaaa gttgatgtgg cagccaattt ctttggaatg
     5761 tctaaagaaa gattagaaaa atacgatgct tctatacctt atggttctga ttctgtatgt
     5821 atagctgtaa aagatggaaa caacgaaatt aataaacttg aagatttaaa aggaaaagtt
     5881 gtagcagttt cagaaggttc tcaagggcaa caggttgcag ctagtgttaa taaagataat
     5941 ttgtttgaag aaaaggttta tggtgatgga actaatggtc ttcaagattt agatttagga
     6001 agagttagcg catggattga tgcagaactt actgttgtag gagacgctaa aaaagctaat
     6061 atgaaggtaa gagtattaga tgaaaaatta tcagttacta atatagcata cttctttaag
     6121 aaaaatgatg aaaaatctaa aactataaaa gaagatgtaa ataaagcaat agaagaaatg
     6181 ctttcagatg gaacagtgaa aaaaatatct gaaaaatggt ttggaatgga tgtaagtgct
     6241 gatatacaaa aataaatact ttaaaattaa aatataaaac tgtgataact atagataagt
     6301 atactaaatt tatctatagt tatctgtgta ttacaatgta tttagattag tctttataaa
     6361 ttaaaatgag ttatggaggt cttgcaaatg attgaaacta aaataaaatc cattgcacta
     6421 aaaaatagag aaaaaatcat aaaaataaga cgacagatac acagtaatcc agagttagca
     6481 tttaaagaat ttaaaacatc caaacttata aaagaagagt taaataaact taaaatagaa
     6541 tacatagatg ttgctggtac aggagtatta gctactataa aaggaaaaaa taatggtggg
     6601 aaaactatat tgcttagagc ggatatggat gcccttccaa tcaaagagga aaatgattta
     6661 gaatttaaat caataaatga taatatgcat gcttgtgggc atgatgccca tgtttcatgg
     6721 ttgttaggaa cagcaatgat tttaaatgac ataaaagaag aattgaatgg aaatgtaaaa
     6781 ttactatttc aacctggcga agaaaaaggt ggttctgata ttgtaattaa agaaaatgta
     6841 cttgaaggtg tggatgcatt agcaactggt cattgttggc caactattga atcaggaaaa
     6901 attggaatcg ctagaaattg tgctatggca gcgactaata cttttgaaat aacaataatt
     6961 ggcaagggcg ggcatggagc agagcctcat aattgtatag acccaattgc agttggaaat
     7021 gcagtatatt catccattca acagatagtt tcaagaaaaa ttgacccagt tgtacctgta
     7081 gtagtgtctg tttgttcttt taattctggt gtttctaaaa atataatacc agatgtatgc
     7141 acacttcaag ggactattag agcgataagc aaggaacaag taattgaagt aagtaaaatt
     7201 ctagaaaata tagtaagaaa tgtatgtaga gcatacggag cagattgcaa atttgaaaag
     7261 agtacaggag gagatgctgt tataaatgac aaaaacatga tagagctagg aagagaaagt
     7321 gcttgcaaaa tacttggagt tgaaaatgtt gaaataattg attttcctgc aatgacaggt
     7381 gaggattttg ctatttatat gaaagaaaaa cctggtttat ttatgtatat aggtgtagga
     7441 aataaagaaa aaaatataaa ctacagattg catagtaaca agtttaatat agatgaaaaa
     7501 tgtcttagta tagcatcttc attattctca caacttgcag tagattattt atgtcaacta
     7561 tagagtacat tcataaaaat atatttagcc tatttttaaa tttaatgcta gtatattaat
     7621 tttgatgtag aacatattat ttattatatt tatttaaaaa ggtggataac aatgataaaa
     7681 gtagaaaatt tacataaaag ttttaaaggg acagaagttc ttaaaggtat aaatttagag
     7741 ataaatcaaa gtgaagtagt agctatactt ggaccatctg gaacaggtaa atctacgtta
     7801 cttagatgtt taaattattt agttgagcct acaaaaggaa agataatgat tggtgatgta
     7861 aaagtagatg tagaaaaagc aacaaaaaaa gatatagcca atttaagaaa acatacatct
     7921 atggtatttc aaaattataa cttatttaaa aataaaacag cattagaaaa tgtaatggag
     7981 gcattgatag tagtacataa aaagagtaat aaagaagcta gagaaatatc tttaaaatta
     8041 atagataaag taggtatgtt ggataggaaa gatttttatc catcaaaatt gtctgggggg
     8101 cagcaacaaa ggataggtat agcaagagcg atggcagtaa atccaaatgt aatattattt
     8161 gatgagccaa catctgcact tgacccagaa cttgttggag aagttttaag cgttataaaa
     8221 tcgcttgcga aagaaggaat aacaatgctt attgtaactc acgaaataaa ttttgcaaaa
     8281 gaagttgctg ataaaattat atttatggat aatggtgtaa tagctgaaga aggaactcct
     8341 gatgaaatat ttaataatcc taaaaatgaa agaactgcaa aatttttaaa tatcgttagg
     8401 aaagaagaag tatttagtta aaagtgtata aattaagtat tagaagatat gtaagatact
     8461 cccattaaat ttataagtta gataataaat ttaatggaag ta
//
LOCUS       DAMJVX010000147         8491 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMJVX010000147 DAMJVX010000000
VERSION     DAMJVX010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8491
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.152"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(22..246)
                     /locus_tag="PC407_002904"
     CDS             complement(22..246)
                     /locus_tag="PC407_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2498331.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(343..1596)
                     /locus_tag="PC407_002905"
     CDS             complement(343..1596)
                     /locus_tag="PC407_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HDF2498332.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIGLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            1856..2845
                     /locus_tag="PC407_002906"
     CDS             1856..2845
                     /locus_tag="PC407_002906"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDF2498333.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEVHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            2917..3657
                     /locus_tag="PC407_002907"
     CDS             2917..3657
                     /locus_tag="PC407_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HDF2498334.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            3857..5008
                     /locus_tag="PC407_002908"
     CDS             3857..5008
                     /locus_tag="PC407_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2498335.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLEPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            5033..5788
                     /locus_tag="PC407_002909"
     CDS             5033..5788
                     /locus_tag="PC407_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HDF2498336.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKNTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            5980..8367
                     /locus_tag="PC407_002910"
     CDS             5980..8367
                     /locus_tag="PC407_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HDF2498337.1"
                     /translation="MKKFTSKKVARFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SDTYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSVKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMDGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVSYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFNIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
BASE COUNT         3396 a          954 c         1454 g         2687 t
ORIGIN      
        1 aaaatctctt tttattatat attaattatt ttttaaattt atattttttg catcagattt
       61 tctaatagct aagtcaaacc ctctaaatct aatcactata gggtcattaa gaggagctat
      121 ctttttcact tcaacttcag tgccatctat tagtcctaag gctagaagtc tcttcataag
      181 tttttcattt ccagctatat tgtctataat tcccttttgt ccaagtttta agttgtaaac
      241 agtcattatt aacacctcct aatgaaaatc attatcaaac tttatattta taatatataa
      301 caaaaagagt aaattgtcaa tatataacaa aaaataaaca tactaagtta gtatgtttat
      361 aaaaaatagt atacatggga tactaatagc atctagcaaa aaaccactta ctaatggtac
      421 aaccaaaaag gcttttggag aatcaccata tttttgtgtc aaagaactca tatttgccaa
      481 agcgttcgga gtagctccta ttccatgtcc aatcaaacca cttattatga tggcagaatc
      541 aaaatctttt ccaagtactc taaagcaaat aaatattgaa aataggatta taaagattac
      601 ttgaaaagtt actattaaga gaataggagg taataatgta gataattcaa ataaatctat
      661 attcataagt gataaagtta aaaaaattcc caatgaaagt tcttttaaaa aatctataag
      721 tttaaaattt aattctaata tatttatctt atcatttaaa tttctaaaaa taacagctga
      781 aaacatacaa gttacaacag agggtaatag tatattaaac gttatataga agcatctact
      841 tataatttct cctgtattta tacaaataaa tatgagaaga acctgttcca aaaatacaga
      901 tggtgttatt gtttcggaga agttattatt ttttatccta ttcttatttc taaaaaattt
      961 acttccaaat ctctttagat ttctattata tctaggaatc gacaagccta tatttgatga
     1021 tggttttaat ttatatttat ttattagaaa tttagctaca ggacatccca atataccacc
     1081 cataataagt cctattgttg cagcagataa tccaacccca actgctcctt ctataccgag
     1141 attttctatt gtaagtccaa atgcagcaga ataaccatga ccaccttcca tagatacatt
     1201 tccacacata agccctaaaa gaggttcaag ttttataatc tttgatattg taatagctaa
     1261 tatattttga caaaatgcca atactccaca caaaatccaa tatctaaata atacactacc
     1321 acctttcttt ataagattta tgcttatttc aagtcctata gttgtaaaaa agaaggttat
     1381 aaaatttggc attaaatttc cacttataga tatatctgct atattaaaat atttaaaaaa
     1441 taaatttaga aaacaaaata atagacctcc tattactggt gcaggtatac agaaattatt
     1501 taaaaagtta actttattct tcaataaatt accaattaaa aagaagaata tagatagtgt
     1561 caaggtttgc actatatcaa aacttaaggt aatcaaaata agtcctcctt tgtaattcaa
     1621 agttttaatt atataactta aattcttatg tattgaaatt ttattatcaa atgtatatta
     1681 ataaatgtat attaaaaaga atcagtatat ataattagat agtcataaat taaattaaaa
     1741 tattttaatg agatgtgact tttttagaat gaaaaaccgt agtatattga tataatagaa
     1801 ataagactta aaaatctgtt gatagtatta tttataagta cagtaggagg ataaaatgtt
     1861 tgaaaattta cctaagatag atttacattg ccacttagat ggaagtgtta gagtagaaac
     1921 catgcttgat atagctatta aagagaaaat agatttacct tcaaataata tggatgaaat
     1981 aaaaaaatta gcaaaggttt cttttaattg tacttcacta gatgaatatt tagaaaaatt
     2041 tgatttaccc ttaaaagtaa tgcaatctaa ggaaaattta aaaagaatta cttttgaact
     2101 tttggaagat gcaagtagag aaaatgtaaa atatattgaa attagatttg caccattact
     2161 tcacacccaa aaaggtatga gtgttaaaaa tataattgag ggtataattg aaggaattag
     2221 agaagctgaa agtatttatg atataaaagg caatttaata ttaggttgta tgagaacaat
     2281 gacctcaaaa gaggcattat tagtaattga agaagggaaa tcatttgtaa ataaaggtgt
     2341 tgtagcagta gacttgtgtg gtccagagaa agagggattt tgtaaggaat ataaagatgt
     2401 ttttaaatta gcaagagaat atggatataa agttacaata catgcaggag aagctgcaag
     2461 tggagaaaat gtattggatg ctataaatat tttaaaggcg gataggattg gtcatggggt
     2521 aaaaattaaa gaccataaaa aggcgtataa tttagttaaa gataaaaaaa tattgcttga
     2581 attatgtcca actagtaatg tacaaactaa gacagtagat tcttatgagg tacatccatt
     2641 ttatactttt tataaagata atttacatgt aagtataaat actgataata gaactgtatc
     2701 agatataaat ttaagttcag aactaaatgt tatatttgat acttttaaac taggattaga
     2761 agactataaa attatttata gaaatgcagt agaagctagt tttgcagata aagaaactaa
     2821 agagtatttg aatagtttaa tttaatagat aaaaatattt aaactttaaa ttaagctatt
     2881 aaattgacaa aaatacattg tattaggaga aaaaatgtga aagattatga agatgttgtt
     2941 tatattaaac tagtcaaaaa agaacttgaa gaatggaaga aaaatatatt aaagaaacct
     3001 tctatatttg acaaagcatc aaagggtata cagaataaat ttaatggagt attgccagat
     3061 aaatatcatg aaataattac ttcttctata aaagtaatga caaaaaccgt actgtttgga
     3121 tataaatatg ctacaaagcc accacttaga aatatgtcat tagaagaaag agaaaaatta
     3181 gttttagaaa aagctaaaac ttacaagacc acagcaatga ttgaaggggc aggaacaggg
     3241 gcaggaggaa tcctcttagg tttatctgat tttcctttgc ttttaggtat taaagtgaaa
     3301 tttttatatg atgtagcttc aatatatgga tatgatgtta aagattatag agaaaggatt
     3361 tatatattaa acatattaca gttggctttt tctagtcaac agcatataaa caaaactttt
     3421 aaattgatga ataactggga tgaatatgta aatagtctac cagaagacat caatgatttt
     3481 gattggagaa catttcaaca agagtataga gattatttag atttagctaa gtttttacaa
     3541 ctaatgcctg gaataggagc atttgtagga ttttatgtga atggaaaatt aattgataaa
     3601 ttatgtgaaa ctgctgtgtc tgcttataga ttgaggattc gagagcttaa gttataagag
     3661 aaattataac ataaaaaatt agttatacaa tatatctata tgtatctaat tttgcattga
     3721 taaaaacaat tatggataaa aggtaaagca tgatagaatc taatgtagag gtttgtattt
     3781 taatggaaaa atttagtttt tattgcttaa gatacaaatt gaattacgaa gagaagtaat
     3841 tgggaggaat attttcatga caaatttgcc aaaacaattt gatagcttca atgaagctag
     3901 acaaaaggga tttataaatg caaaggaact taaggaaagt gggaaaaaga tggttggagt
     3961 tttctgtaca tttactccag ttgaaattcc aatggcagct ggagcaactg tagtaggtgt
     4021 atgcggtgtt agtgaagagc caatacctga tgcagaaagg gtgcttccaa gaaatctttg
     4081 tcctttaatt aaatcaagct atggacatgc aataacagac acctgtcctt atttctattt
     4141 ttctgatttg ttgataggag aaaccacttg tgatggtaag aaaaaaatgt atgaagaact
     4201 tgcaaaagtt aagccaacat atgtaatgca tttgccaaat acttcaaaag gagaatttgc
     4261 ctataaactt tggaaagatg aaatgataag attaaaagaa gaggttgaga aatctttagg
     4321 tgtaactata actgaagaag atataagaac tgctataaaa gataaaaatg aagaaagaga
     4381 acttttaaaa gagttttatg cacttggtaa attagaacca tctgcactta caggccttga
     4441 gttacataat gttttatatc aagcaggttt taaatttgac cgtgcagaac ttaaacattc
     4501 tttaagaaaa gttatagatg atatgaaaga aagatatgaa aaaggtgaat gtcctgttca
     4561 aaaagataag ccaagaatat tgataacagg ttctcctatt ggtggaataa gcgaaaaaat
     4621 agttaaaact ttagaagatg caggggcatc agtcgttgca tatgaacttt gtggagctat
     4681 tagatcaaac gatttattgg ttgatgaaga aatagaagat gtatatgatg ctttaacaca
     4741 aaaatatata aatataggtt gttcttgtat gatgaataat gacaatcgta ttgaattatt
     4801 agatagaata atagatgaat ataatgtaga tgctgtaata gatgtagttt tacaagcatg
     4861 tcatacattt aatatagaaa gttatagaat aagagaattt gttacaaaag aaaaaaataa
     4921 gccatttatg tctcttgaaa ctgattattc aaaatcagat actgaacaat taagaactag
     4981 atttgaagcg tttgtagaga tgttgtagga ataaataaaa gtaggaggaa ctatgtttag
     5041 tataggagtt gactcagggt cagtggcaac aaagggtgtg ctatttgatg gtgaaaaaat
     5101 tattaagaaa ataattatac caactggatg gagccctaaa aatacatcta aacaggtcta
     5161 tgagttgcta agtagtgaaa ttgacaaaaa agatataaaa aaggtggtag gtacaggtta
     5221 tggaagggga gtaatggatt ttgctgataa gaaagtaaca gaaataactt gtcatactag
     5281 aggtatttac tttttaaaca agaacataag gactatttta gatgtaggtg gtcaagatag
     5341 taaagttata aatttagata gagatggaaa tgtatttaac tttataatga atgataagtg
     5401 tgctgcagga actggtcgat ttttagagat tacttctaac cttttaggaa gcgatattga
     5461 aagtatagat acattagcta aaggatatga accagttaat atatctagta tgtgtacagt
     5521 ttttgctgag tctgaaattg taagtcttct agctcaaaac atttctactg gagaggtagc
     5581 agcagggata cttaaatcta ttgcaaataa atccacctca atgttagcaa gaggtgaggt
     5641 aatagatgaa gttgcattta ctggagggct tgcaaaaagt aaagaactag taaaaatgat
     5701 agaagaaata ctgggtaaaa aaatttttat tgcagaagat actcaaataa taggagcttt
     5761 aggagctgct gtaataggat ttagataaaa acatataaaa agaaaatgaa aagctgaatt
     5821 tatcataaat aagttcagtt ttttatttta ttttgtttat ttataaagga aaactttggt
     5881 ataattgatt taaatgattg tatatttgta aatacttgat atttagtcat atataacatt
     5941 agaattttaa aaattagctc aaaataaggg ggagaaagcg tgaaaaaatt tacttcaaaa
     6001 aaagtagcaa ggtttgttgc actgttttta atatctattt tagtaatgtc aacaattcct
     6061 gtgtcagctg acaatagcaa tacattgaat cagataaaaa aagcagaata cagtgatact
     6121 tataaacaat atctaaatga tgctaaaaat ggacgtacag aaaaatataa tggaattata
     6181 ccaaatccat atcagttaga aggtactcaa attcaaagta aaggaagaac agctccaaca
     6241 tcttatgacc ctagaaaatt aggtcttatg acatcagtaa aaaatcaaga ggatttagga
     6301 atatgctggg attttgctgc aatggcaacc ttggaaagtt tcttgaagtt aaataattat
     6361 ggagactacg atttatcaga agaacactta agatggtggg cttcagatgg tgaatatgga
     6421 tggtctgtaa atgatatgga tggtgcttta aattatgaag ctatgggata tttaacatct
     6481 tggtcaggtc ctaaattaga aaaagatatc ccatacaatg gtagagtatc taaagcacaa
     6541 ggagcaaaaa aaccaactaa tatgaataca gcacctactg tttttaatgt gacagatgct
     6601 gtgtgtgtaa gtaatgacat aaattctaca aaaaatgcta tattacagta tggagcagta
     6661 acatctggtt attatgaaga tattaaatat caaagtgatg accaaaattc atattattgt
     6721 ccaacaaaaa gttttaatac aaatcatgct atttcaattg tgggttggga tgataactat
     6781 tctaaagata attttaatgc aaatattaga ccatcaaaaa atggtgcttg gttaataaaa
     6841 aatagctggg gagattataa ttctgagggt ggatatttct ggatatctta tgaagacaag
     6901 acacttatgt ctgatataga taatttttca atcaaaggtg gtaaaaaacc aaatgatgat
     6961 gagaagatgt atcaacatga ctatgcaagt attgtacctt taatttcaaa aaagataaca
     7021 gctgcaaatg tatttgattt caatagaggc gatgaaactc taaaatctgt aatgttctta
     7081 actgaaagta taggagctaa gtatgaagta tcttatgctc cagtagtaaa tggtatacct
     7141 caagaaaaca atatgaaaaa attaaaagaa ggtactgccc aatattcagg atacataaca
     7201 gttccaattg attcttttaa tataccagaa ggaaagggtg caattgtagt cagtatagag
     7261 gggaaaaatg gagagtcaac tataggttct gagtcaaatg taccaggata tgatacattt
     7321 aaagcaaaag ctaatttagg tgaaagttat atcatagata atactggaaa attctttgat
     7381 ataaatagag atagtaactt ctatccgtgt aattttacta taaaagctgt tacagaaaag
     7441 tcttctggtg agagtatccc gaatgagtct ttaattggta gtgatagata tgaaactgct
     7501 atcaaagtta gtcaaaatgg tttcaattct tcagaaaatg tagttttagt aaatggcagt
     7561 tcaatagtag atgcattggc agcaactcct tttacatctg ctataaattc accaatatta
     7621 ttgacacaaa aagaagcttt aaattcaaaa actaaagctg agatacagag attaggtgct
     7681 aagaaagtat acctaatagg cggagaaaat tctataagta aagaaattga acaacaatta
     7741 aaaagcttaa atatatcgat agagagaata tcaggaagtg atagatacaa gacaagttta
     7801 ttattagctc aaaagttaaa tggtataaaa aatgtatctc aaatagcagt tgtaaatgga
     7861 gtgaaaggat tagctgatgc aattagtgta ggtgctgctg cagcagaaaa taacatacca
     7921 atcatacttg caaatgaaaa gagcgaattg caaggagctg atgaatttct aaattcatta
     7981 aatatagaga aatcttatat tattggagga acagcatccc tatcaaacaa tctagaaagt
     8041 aagttaaaaa atccaactag attatctggt agtagtagag acgaaactaa ttcaaagata
     8101 atagataatt tttataaaaa agatacttta aaaaatgcat ttgtagttaa aaatggaatc
     8161 aagaatcaaa atgatttaat agatggattg gcagttggtc ctttaggagc aaaaactggg
     8221 tcaccagtaa ttttagttgg tgataaatta gctgatagcc aaaaagaagt tcttaaaaat
     8281 aagactttag aaaaagtaac tcaagttggt ggaggagcaa ataaaaatgc atttaaagag
     8341 ttagtaaaat taaaaagtgc taaatagagt acttaagtat taacatatat tagtgtgtat
     8401 tagtaactta tcttaataaa agctgaaaat aattacttta aattattgat aaataagata
     8461 atcaatgatt gtaaagtttt gttttcagct t
//
LOCUS       DAMJVX010000148         8472 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMJVX010000148 DAMJVX010000000
VERSION     DAMJVX010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8472
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.153"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            261..1247
                     /locus_tag="PC407_002911"
     CDS             261..1247
                     /locus_tag="PC407_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2498338.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPMVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1470..1553
                     /locus_tag="PC407_002912"
                     /pseudo
     CDS             1470..1553
                     /locus_tag="PC407_002912"
                     /inference="COORDINATES: protein motif:HMM:NF012587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1584..3065
                     /locus_tag="PC407_002913"
     CDS             1584..3065
                     /locus_tag="PC407_002913"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDF2498339.1"
                     /translation="MYKEPKYRTILESIKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSVLEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            3091..4062
                     /locus_tag="PC407_002914"
     CDS             3091..4062
                     /locus_tag="PC407_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDF2498340.1"
                     /translation="MKKVISIGEALIDFIPNQNGCKLKNVSEFRRVAGGAPANVSVVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFMTGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4318..4920)
                     /locus_tag="PC407_002915"
     CDS             complement(4318..4920)
                     /locus_tag="PC407_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2498341.1"
                     /translation="MEICILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEKHSRQFAKQIYYILDNSIEVDNC"
     gene            5201..6412
                     /locus_tag="PC407_002916"
     CDS             5201..6412
                     /locus_tag="PC407_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HDF2498342.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKYDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIVFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6480..6677
                     /locus_tag="PC407_002917"
     CDS             6480..6677
                     /locus_tag="PC407_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HDF2498343.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            6711..7682
                     /locus_tag="PC407_002918"
     CDS             6711..7682
                     /locus_tag="PC407_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2498344.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            7869..8246
                     /locus_tag="PC407_002919"
     CDS             7869..8246
                     /locus_tag="PC407_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2498345.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3441 a          875 c         1361 g         2795 t
ORIGIN      
        1 gttgtaaaaa tcatagcctt atttttatat tataaataaa ttagagtatt tatcaattca
       61 tagtagataa aattgaaatt aaatataaca acaaaaaatg aaaattacaa ttaatttaat
      121 gttaaagact caattatttg gatgtggaat ggatagtaca agtagtatat aaaaagtatt
      181 gactggacaa ataactatat tttattttaa atatataata taatgtaata aaatatatca
      241 aatgtagaag gtgataacct atgaaaaaaa taacaataaa tgatattgct aacttggcag
      301 gagtatctaa aagtactgta tcaagatatc taaataataa agatatcagt gattctacta
      361 aagagaaaat aaagacaata atagatgaat atggatatga gccgaatgca tttgctcaaa
      421 gtttgagagc taaaaaaaca tactttattg gtattataac accgtgttta gattcttttg
      481 taaaatctaa aattatgatg gcaatagatg aagaactcaa agaattaaaa tatacatcac
      541 taattattaa tactaataga aaaataaggt cagaaataga tagtatatca aagttagcta
      601 gtctaaaagt tgatggaata atactaattg gtaccgaaat aactaaagaa cataagaatg
      661 aaattgaaaa gttagatata ccaatggttg tagttggaca aaaagttgat ggtatcaata
      721 gcatagtgaa tgatgattat ggagcaggat ataaaatggg tcaatatatt gctaataaag
      781 gttacaaaaa cattgtatat ttaggtgttg atgaaagtga tatatcagtt ggtttaaata
      841 gaaaaaatgg agtacttaat gggttaaaag ataaagggta tgatgcaaaa gtattttata
      901 ctgactttga ccaagaaacg tctatacaaa gaagtggaga aatgctagaa agtgaaaatc
      961 cagatataat aatatgtgca actgataata tagcaatagc aacaatgaaa gaaatcaata
     1021 agagaggtaa aaatatacca caagatatat ctgtagctgg atttggggga tatgatgtat
     1081 tgtctattat aagccctaaa cttacaacaa taaagtttga aaataaaaat gcaggtaaag
     1141 tagcagcaaa tacgatagtg aacttaatac aagaaagaaa agaaccacta ttaaaggaga
     1201 ttaaatttga attaatagaa ggtgaaagta caataaataa aaattaagat acacattaca
     1261 tatattagta atgtgtattt ttttactaaa aaaactataa ttctaaaaat taggaaaact
     1321 tttttgaaag aaatattgca tctgaaataa atttatgcta atctttaatt aaaccggttt
     1381 cgaaaccggt ttaattaaac cgaaagaata gcacaatgag tgcaaaaata actgattgta
     1441 acaaagtatc aaagtaatta ttagagtaca taggaaataa acaaaatata aaaggtgtag
     1501 cacattgtgt aacaagatta agaattgttt tagatagtaa taataaagca aatataaaat
     1561 tacaaaagaa aaggaataaa aatatgtaca aagagcctaa atatagaacc atattagaat
     1621 cgataaaaga agaattaaat aaattaagaa atatatcttt aaatgataaa tataaaccac
     1681 tatttcatat acatccacaa catggactac ttaatgatcc aaatggatta gcatattata
     1741 atggtaagta ccatgtattt tatcaatggt atccatatga tgcaactcat ggaatgaagc
     1801 attgggctta tgtaagttct gatgattttg taaactggaa tagagaagat gtagcattaa
     1861 taccaatcga aagttatgaa tctcatggag catattcagg taatgcgata gaggtagatg
     1921 gaaaattgca tatgtattat acagggaata taaaatatag tgcagaagat agatatgcat
     1981 atcaaaactt agctataatg aacaaggatg gtaaaataac taaatatgaa aataatccaa
     2041 tagtaagtga aataccaaaa ggatatacag gacatgtaag agacccaaaa gtatttaaaa
     2101 gaaaggataa atactttatg ttacttggag cacaaacaag tgataaaaaa ggagttataa
     2161 tagtatatga atctaaaaac tctatagatt ggaattttaa aggagaatta aatgtaaaaa
     2221 atatagatga agattttggt tacatgtggg aatgtccaga ttatattaat atagatgaaa
     2281 aagatatatt aatattttcg ccacaaggag tagaacctaa aggatttgat tatcaaaata
     2341 tatataatgt agtatatgct attggtaata tggatttaga taatttaaca tttgaaatag
     2401 atacaatgaa agaactggaa aaaggatttg atttctatgc tccacaaaca tttataaagg
     2461 actcacaaat aatactattt gcatgggctg gaatgggaga agttttatac cctacagata
     2521 aaaacaaatg ggcacattgt ctaactgttc caagaaaatt aaatataaaa aataacaagc
     2581 tattgcaaat gcctgtagat gaattaatta aattaagata tgatgaaaca agtgggcaaa
     2641 atactataaa gaataatatc aatattatag aaaatgatga aaatgtatat gagcttaata
     2701 ttaatataaa aaatattgac agcaataaat ttgggctgga attattttca tcacaagatg
     2761 aaggtgtaaa attagaattt aacaaattag gaaacattgt aactttagac agaagcaact
     2821 ttaaaaaagt tttcggtgtt gaatatggaa caaatagaaa agaatatata aatattgatg
     2881 aaaatactaa tatcaaagta ctagcggata gaagtgtttt agaaattttt ataaatgatg
     2941 gagaagtagt atttactagt agaatatttg ctaaagaaaa ttctaatcaa ataagggttt
     3001 atagtgataa aatagtagag tatgagtata ctaaatttaa attaaagcaa ggtattgaat
     3061 tataaaaaca cattataagg ggtagaaaag atgaaaaaag taattagtat aggagaagct
     3121 ttaatagact ttatacctaa tcaaaatggt tgtaaattaa aaaatgtatc agaatttaga
     3181 agagtagcag gaggagctcc agcaaatgtt agtgttgttg tagcaaagct tggaggaaaa
     3241 tcaagtttta tatctaagtt aggaaaagat gcatttggtg attatataat agatgtatta
     3301 aatgaagtaa atgtaaatac tgattatgta ttgagaacaa gtaaagcaaa tacaggactt
     3361 gcttttgtat cattaaaaga agatggtaat cgtgatttct cattttatag aaatccaagt
     3421 gcagatatgc ttttagaagc tgatgaggtt aaaaaagagt ggtttaataa ttgtcatatc
     3481 ttacattttt gttcagtaga tttaatagat agtccaatga aattagcaca taaaaaagca
     3541 atagaatacg cacttgaaag taatagtata ataagttttg accctaatat aagattacct
     3601 ttatgggata gtgagcaatc ttgtaaaaag gcaatatcag aatttttacc ttttgctcat
     3661 attgttaaga tatctgatga agaattagaa tttatgacag gagaaaatga tatagaaaaa
     3721 tcattacata aactctttgt ggggaatgtt gaactagtat tatatacaaa aggaaaagat
     3781 ggagtagatg catacactaa aaaagtaaaa ggtatgtgta aaggggttaa agtaaatgca
     3841 atagatacaa ccggagcagg agattcttat ataggttcat ttttatacac cttattgtat
     3901 aaacaaataa cattgaatga tataaaagaa atgaaacaag aaacgttaaa tgagtattta
     3961 gaattttcta attattatgc agctcgtagt acaactatta aaggtgcgat aagttcatat
     4021 gcaacaaagg aagagataac tgaatttata aaaaatttat agtaggaatg agtaataggt
     4081 tttgaaggaa gtaacatgta aagagttttt aaggatgttt actatgaaaa taattaacat
     4141 ataaaaagca ataaagaggc tgtctcaaaa tagatataag cctctttatc attgttgaaa
     4201 tgataaaaaa tattttaatt attgttactt tttagaagaa tatatatctt attttaaata
     4261 agcaaattat gtaagattta tttatattat caatacttta cttggagtat tatgtcttta
     4321 gcagttatct acttcaattg aattatctaa aatataataa atttgttttg caaattgacg
     4381 agaatgtttt tctttttctt tattcataaa attaggttta cctaaatcac gttcaacaag
     4441 cattcctata tattttaact gtgaatgctt acagtagcgc ttcataccat tttcccaaat
     4501 atcagcaccc ttttcaggtc tatatccaca tgttgtaata agtgctactg gttttcctgc
     4561 ccatagagcg gggcctttct cattaccata gtacttattc attccataca ccaatctgtc
     4621 aagtagtgct ttcattggag aagtgcagta ccaagaataa attggagtag caaaaacaat
     4681 taaatcacac ataagaattt tcttgaaaat tttctgaaca tcatctttac aataacatcc
     4741 aaaatcaaat aagttatcct gacatttttt acaagcaata catggctcta ttttacaatc
     4801 atataaccat attacttcaa tatttgaatt ataattttct aattcctcaa taaaaggttt
     4861 taaaatagca gcagtattac cttttttctt aggacttccc ataagaatgc atatttccat
     4921 attgatacct ccacagtatt ttaaaatagt ttttagtgct taatatagtt ttaggatact
     4981 atgagaatac gttaacaaca atacactttg tatattttta ataaaattga aacaaattgt
     5041 tttatcaata aaagttggaa tgtatctata attaaaaata ttttaggtat gtttttacat
     5101 atatgtgtgt agttaactat ggtaatttat tgtaaaatta tcttatagga ttataattgc
     5161 ttaatttatg attatattta aaatcttgga ggtaaaaaat atgtactcaa atgaagacta
     5221 taaatttata gaagaaaatg caccatgtta tatatatgat gagactaata taattgaaaa
     5281 atgtaaggaa ttgaaaaata atattttagg ttttgatttt ttatattcta taaaaacaaa
     5341 tccatataac aaaattgtaa aaagcattgc agagcaaggt tttggagcag atgcttcttc
     5401 aactaatgaa gttatgattt catcagaatg tggagtttct aaatatgata tttattattc
     5461 atcaccaggc aaaacagaga ttgatataga aactgtttta gataaatgca taattattgc
     5521 agatagtatt acagaaatag aacttataaa taagatatct aaaaagcata atcagaaagt
     5581 taaaatagga ataagaataa atcctgattt tactatggat gatgatagtg ctaaatcaag
     5641 caaatttggt atagatatag aagatatgag cagtgttttt aagatgttaa gtatgtgtga
     5701 ttatgtatct gtagttggga ttcatataca tattaaatct caggtattgg attttgaaaa
     5761 gttaggacat tattatgaaa aatgtttttt tatagcttta aatttaaata aaagtaagta
     5821 tattaatatt gaatttataa attttggtag tggacttgga gtagcttatg ataatcttag
     5881 agaaaatcca cttaatctta aagagctttc aaaattaact caaaagattg tttttactaa
     5941 ttcaaacaca ctgaaatcaa gacttcttat tgaatcagga cgtttcatag tttgtaaatc
     6001 aggtaattat tatgttaaaa ttgtagatat taaagtatca agagggaaaa aatatcttat
     6061 tgtaaaaaat ggacttaatg gatttatgag acctattatt tctaatttaa ttgaaaatat
     6121 tacttgtgga gtagaattag aaggtcaaga gccattatat acatcaaatc atgccttttc
     6181 tctttcagta ttaaataatt taaaagaaga agagtatgta aatgttgttg ggaatctctg
     6241 tacatcatta gatgtaattg caaataatgt taaattgaaa aaagcaaata taggagattt
     6301 gatttcaatt agtaatgctg gaagttactc ctattcttta tcaccactat tattttcgag
     6361 ttatgaattt cctaaacaat attataaaag aaaagatgga tatattgagt aaacaatagg
     6421 ataaattaat aaacttagta ttattgaaaa aatttaaaat gcagggggtg taattctata
     6481 tggaattgtg gaaaagaaat ttatttgtat gttggatagg tatgtttttt agttctatag
     6541 ggatgagcca aatagcacct atacttccat tatatattaa acagttgggg gtaactgatg
     6601 ttagtttaat acaacagtat tcaggtataa tttttggatg tacctttgta gtagcagcat
     6661 tcttttcgcc aatttgaggg aaagctgctg ataaatatgg aagaaaatca atgcttttac
     6721 gtgcaagtat tgggatggga atagttatat ttaccatggc gtttgttcaa aatgtttatc
     6781 agttattagg attaagaata cttcaaggag tttttacggg ttatgctact gcatgtacta
     6841 ctcttattgc aacacagaca gataaaaatc attcaggatg ggctttgggt actcttgcaa
     6901 ctgcatcaac tactggctca ttaattggtc caacagtagg aggatatata gaatcaatct
     6961 tgggtcttaa aagtacattt attataacag gaggattgtt gtttgtatca tttatcatct
     7021 ctattttatt tgtagtagat aattttaaac ctaaagaaat aaaagaatca atttctatac
     7081 ctaaagaaca attaaatata ttgccaaata aatttttaat tgcatctatt tttgtaacca
     7141 cattcattac tcaactagct ttatactcta tagaaccaat tgtaaccata tatatatcac
     7201 aacttacaaa ttttgcatca aatgtagctt taatagctgg attgacattt tctgcatcag
     7261 gattagcaaa tttattagca gctcaaaaac ttggaaaaat gtcagataaa ataggtcctc
     7321 aaaaagtatt gttaatttca cttttatggg ctgggattat atttatacca caagcttttg
     7381 taaaaactgc atggcaactg atgttgttga gatttttatt agggttgtca gttgcagggc
     7441 ttaatccttc tgttaacagc ttgcttaaaa aaattgcacc agaagaatat gtgggaaaaa
     7501 tatttggata taatgcatct gcccagtata taggttgttc atcaggagca tttttagggg
     7561 gacaaatatc agcacattta ggtattagaa cagtattttt ttcaacaagt ttattgttgt
     7621 ttatgaatgc actttggatg tataaattta ctggattgtt tattaaagat aagactaaat
     7681 aatcatacta tttacatttc taattttatg atgtaaaata aatatttttt atgtaattta
     7741 tcatagaatt atataaaaaa taactctatg gtaaaaattg gaaaatatta ttatcgacaa
     7801 aatctttgac ttaaaaacta tattgcgata gtattaattt aataaaagtt tgagaggggc
     7861 gtaaggttat gacaatttca aaaataccac aagcagaatt aaaagttatg aagtttattt
     7921 gggaaaaaaa tgatacagta acatcaaaag aaactattga agctatggaa cgaaaatatg
     7981 gttggaaaca aacaactaca ttgacacttt tatcaagact tgttaaaaaa cattttttaa
     8041 gtgcagaaaa aatagataga tacactcatt atacaatagt tgttggacat aaggagtatt
     8101 taagtgttga gacaaaagat tttttaagca atatacacga caattcttta cagagtttaa
     8161 tttcagcact acatgatgat gaagttgttg ataaagatat gttggattta tttgaaagtc
     8221 attttaaaaa tttagaagaa gagtaattat atcaggataa aactctaaga atttaattaa
     8281 tctatggctg aaaaccataa atggtattat ttaatcagct actcactatt cataaagtaa
     8341 tattaaatag gttctctaat tagtaaaata cctaaaataa ttggaatagg gagtatcaag
     8401 ttaaatgata ccacttattt aaataagaaa tgatttttaa aatttaaaat aattttataa
     8461 cttgtcaata tt
//
LOCUS       DAMJVX010000149         8454 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMJVX010000149 DAMJVX010000000
VERSION     DAMJVX010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.154"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            69..2180
                     /locus_tag="PC407_002920"
     CDS             69..2180
                     /locus_tag="PC407_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HDF2498346.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDNWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTAL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(2378..3343)
                     /locus_tag="PC407_002921"
     CDS             complement(2378..3343)
                     /locus_tag="PC407_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2498347.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(3628..4584)
                     /locus_tag="PC407_002922"
     CDS             complement(3628..4584)
                     /locus_tag="PC407_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2498348.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVDIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            4952..5740
                     /locus_tag="PC407_002923"
     CDS             4952..5740
                     /locus_tag="PC407_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2498349.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVDDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(5863..6333)
                     /locus_tag="PC407_002924"
     CDS             complement(5863..6333)
                     /locus_tag="PC407_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HDF2498350.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            6472..7116
                     /gene="uppS"
                     /locus_tag="PC407_002925"
     CDS             6472..7116
                     /gene="uppS"
                     /locus_tag="PC407_002925"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDF2498351.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            7159..8064
                     /locus_tag="PC407_002926"
     CDS             7159..8064
                     /locus_tag="PC407_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2498352.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQKVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
BASE COUNT         3299 a         1013 c         1341 g         2801 t
ORIGIN      
        1 aattttagat atacttaata agaagtatat tgtagaaaaa ataaatttaa aaaattaagg
       61 gggatgaaat gaaaaaaata agtatattag tattatcatt gataatgaca cttactatgt
      121 gtagtgtaag ttcatttgcc gattcatcaa atgataagga aatgagagct gcttggatat
      181 ctacagttta taatttagac tggcctaaga ctaagaataa tgaagccaag caaaaaaagg
      241 aatacacaga tttattggac aaactaaaaa gtgtaggaat aaatacagca gtggttcaag
      301 taagaccaaa atcagatgct ttatataaat caaatataaa cccatggtct gaatatctta
      361 ccggtacaca aggtaaagac cctggatatg acccattacc atttttaata gaagaagcac
      421 ataaaagagg aatggaattt catgcatggt ttaatccata tagaataact atggcagatg
      481 agagcataga taaattgcca gcaaaccatc cagcaaagaa aaatccaagc tgggtagtta
      541 aacatggaaa taagtattac tacgacccag gtttaccaga agttagaaag tacatagtgg
      601 atagtatagc agaagtagtt caaaattacg atatagatgg agttcatttt gatgattact
      661 tctatccagg agtaagtttc aatgatactg caacatacca aaaatatgga aaaggacaaa
      721 ataaggataa ttggagaaga gaaaatgtaa atactctact tagagatgta aaagcgtcta
      781 taaagagtat aaagcctaat gttgtatttg gtgtaagccc agcaggtata tggagaaata
      841 agagtagtga cccaacaggt tcagatacat caggtaatga aagttatgta ggtacatatg
      901 cagatacaag agcatggata aaacaaggat tgattgacta tgtagttcca caactttact
      961 ggccaattgg tcttaaagca gcagattatt ctaaattagt ggcatggtgg gctaatgaag
     1021 taaaaggaac aaatgtagac ttatacatag gacaaggtat atataagcaa ggtcaatcta
     1081 gttatggtgg acaaaatata gcaaaagaaa tagtacaaca agtaacatta aatagaaagt
     1141 atagtgaaat aaaaggtagt atgtatttct cagctaaaga tatagcaaat agtacttcta
     1201 tacaaaagga tttaaagagt ttatattcta gtagtgaaga accagttact ccaccatcta
     1261 atgttaaagt ggagaaatta agaggcgacg aaagatatga cacagcagta gcaataagta
     1321 aaaaaggttg ggctacaaat tcagatacag tagtattagt aaatgggtac tctatagttg
     1381 atggcataac atcaacacca cttgctacat caaatgatgc tcctatacta ttggtaaata
     1441 aggataatat acctacatcc actaagaatg agttaaaaag acttaatcca agtaaagtta
     1501 ttttaattgg aggaaataac tctattggag ataaagttga gagtgagata aaagatacat
     1561 tgtcaaatgt atcaataaat agagttggtg gttcagatag atatagtaca tctttaatga
     1621 tagcaaaaga attagtaaaa actaatccag tagaaaaact atatataaca agtggtacag
     1681 gtgaagcaga ttctctttct atagcttcaa aagcaggaga agaaaagcaa ccaatagtac
     1741 tagtatcaaa agataatgta agtgatgaag tttacaattg gatatcagat ttaaaagtaa
     1801 aagatgctta ttttataggt ggtaatctaa gtatatctga ttcagtaata aataagctgg
     1861 ataaggtcat tacaaatgat gtatctaaaa atagaatagc tggagaaaat agagaagaaa
     1921 caaatggaaa agtaatacaa aaattctatc caaatgctga atattcatca atgtttgtaa
     1981 gtaaaagtaa tcaattggta gatgcattaa catcaggtcc tttagcagct aaattaaaat
     2041 caccagtagt tatgcttgga aatagtgtaa catctgctca gaaaactgct ttagagccta
     2101 agaaaactgc tctagtatac gaagctggag atggtataaa tcaaaacaca ttaaacactt
     2161 tcttaaactt agttaagtaa taaaaaagtt aatttaaaat acagagtatt ttataaatgt
     2221 agattaaaat gaaaattatc atcataaatt agaaaataaa aaagaactat atgcattttg
     2281 taatcaagtg tacacagcaa cttgaaaata attatgcgat atagttcttt tttagtaatt
     2341 aatcttatac tttttaataa actttatgtt gaatttttta ttgtttaaac tctatgtcat
     2401 taaatataaa atcgtgtaat atgtctaaag tatctgggtc aaatctaaga acccatccag
     2461 cttttccata tataccacct gtactatgaa tttcatcatc aataggaaat tcaaattgct
     2521 taatatttaa gtcacccatt tttaaaactt gagctcctaa agaaagtatt gcattaggct
     2581 tcatgttagt tttaacatat ggaagaactg catctaataa acctggatat ttagtaacag
     2641 gtaaagtttc cgttttcttg attatagctt gtataatctg tctctgtctg ccatctctag
     2701 caaaggctgt atcgttgtgt ctgatacgtg caaaagatag tgcctgatat ccatttagag
     2761 tctgctttcc agagtttcta atgtattgta tgctaccttt atcatcactt ttattccatt
     2821 tataagtttc aggaataaat ttatttagtt ctcgtatttc tcctttttca acagtaacct
     2881 caacgccacc aatagcatca attatataca taaatgattc gaaattaaca caagcataat
     2941 tttgaatatc aatattaaaa ttctcttcaa ttgtttgtat taataaatca gcttgtccat
     3001 aagcataagc atgagtaagt ttttgtttac catgaccagg gatgtctaca tagctatcac
     3061 gagctaatga tgtaagtttg atatcattgt gtttgttatc aatagtaagt atcatcatag
     3121 catctgacct tgaagaatct tcatttggtc tagcatctgt tcccataagt agtatgttaa
     3181 ttatcccatc ttcattttta tggtcattgt tatccaataa atcactactt atgctcgagt
     3241 catgaatagc agataattta taataaaaat atccatagac acttataggg aatattacta
     3301 ccaagaaagc cagtagtata acaaatttct ttaattttga caaaagtacc ccttctttct
     3361 tctttttcct gtatattttt taatagcata cacttaatta tattaaagta ttgagatata
     3421 ttcaatactt gtttcagtta caaaaaaaag acaatgatat aatttataag aaaatttatc
     3481 gacctatatg tatagttaaa agtatatata atatattttt taaaagtaat ttatatttta
     3541 aaataaaaac acaccccata aatacaaagc aatttgcatt taagaggtat gtatttatca
     3601 tcaaattatt ggtttgtttt ggaatttcta atcttcaacc ataatatctt taaatatgaa
     3661 atcatgcatt gcatctagtt catattcctc aaaaggtatt acatagcctg cattacctat
     3721 ttttctacca ttttcagtag aaagaggaaa ctccatagat ttcaaattta aatttcctat
     3781 acttaaaagt tcttttccta aagatattat ttcattagga ttcatattag tttttacata
     3841 cggaagtatt gtgttaacta gattaggata tttagtgact ggtaaatcct taacaccatt
     3901 tattaaactc tgtataacac ttctttgtct tctatctctt tcatctgtac tatcattttt
     3961 tcttatacga gaaaatgcaa gtgtctgata tccatttaat ttttgctcac cagaatttct
     4021 tatatattta agagaacctt tattgtcatt tttattccat ttataagttt caggaataaa
     4081 tttatttaat tctcttattt cactcttatc aacagtaaca gtaactccac ctaaagcatc
     4141 aactatatcc ataaaagaga aaaagtcaac tacggcgtag ttttgtatat caagttggaa
     4201 gttttcttct atagtttcta ttaataagtt agcttgtcca tatacgtagg catgtgttaa
     4261 tttttgttct ccacgtcctg gtatatcaac ataagtatct ctatttaatg aagttagctt
     4321 aagactttta tttttattat caatagtaag tatcatcatg gcatcagaac gagaaacttc
     4381 ttctcctggt cttgcatctg tacctaacaa taatatgttt gtgatatttt cttcgttttt
     4441 atagtcatta ctactaagcg cctctgtatt tatactatca tcttgtatag tttttaattt
     4501 agtaaaaaca tatccatata tactaacagg aaatgctata actaaaaggg ctagaaatat
     4561 aacgaacttt ttgagtcctg acaataatat cccctacttt ctaaattttt taattacaca
     4621 tagttaatta tattaaaaat ttgttacata agcaataaaa aacacaataa attagcataa
     4681 gtttgtcata atataattat ttttatagta ttttatagaa gaaagtgtgt agttattatt
     4741 gattttattg gaatataatt gtatgaagac ttaaattttt ataaactgat gtgtatattg
     4801 aaaaaaatat tttcctgaat gtagttatag catcttatga aaggtgtaaa ttgtaattta
     4861 aaatttttag tgtataataa gaattgaaaa ttcaaaccga taaaaattaa taaaaaataa
     4921 ttataaatta ataatttggg ggaggaaatg tatgataaaa ttaatagcaa cagatttaga
     4981 tggaactttg ttagatgaaa agagcgaaat aaatccagag ttttataaag tatttaaaaa
     5041 attaagagag agagggataa tgttttctgc tgctagtggc aggcagtatc aaaatcttat
     5101 aaaaaaattt gaagatataa aagatgatat gatgtttata tcagaaaatg gaacactagt
     5161 agtatataaa ggaaaagaaa tattatcaaa tcctttaaat aaagaattag taaatgaaat
     5221 catagagaca acaagaagta taaaagggaa aaaaattgta atgagtggga aaaaatacgc
     5281 ttatatagaa agcaaagatg aagcgtttat acaagaggtt agtacttatt atgcaaagtt
     5341 taaaattgta gacgatttga cgaaagtaga aggcgatata ttgaagatag ctgtatttga
     5401 cttcaaaggt gcagaacata ataataatat ctactttgaa aagttttctg atagagctca
     5461 agtatgtatt tctggagtgg agtggcttga tttaactgca aaaggtgcaa ataaagggag
     5521 tgccataaaa aaagtacaaa aaatgttaga tataaaatat gaagaaacta tggtatttgg
     5581 tgaccagtta aatgatgtgg aaatgatgaa aagtgcctat catagttatg ccatggaaaa
     5641 tgcaaatgaa catttgaaac aaattgctag atttagagca aaaagaaata ctgaaaatgg
     5701 cgttgtagat aagataaaag aggtcataaa aattggatag tcatatgtat agatgaaaaa
     5761 gattattttg aagaaatcaa aaaaatatca atgtaatttt gataaataag tttatttatg
     5821 cgaaaattac attgatattt aataaacatc aataatagat atttattttt ttatgatatt
     5881 cagtattttg ttttctattt gagttgcatt ttcagcagaa attaaataaa catatctacc
     5941 tgctttctca attatagtat tatttgctat ttcagaattg attttttctc catgtccttc
     6001 tccaaaaggt gcttgtatca tatctttttt ataattttct agagttttat atattttatc
     6061 tacctcgtca gtttttataa cgataatatc ttctagagat aatttttcag aagaattaat
     6121 tacaaaacct tgaattatgt cttcctctac atcattaaaa tagttaaagt cctttgcatc
     6181 aaagattttt gattctttta taagtaaatc agaagatttt aatgcttttt ttatttttga
     6241 tgtagaaacg tttttgtaat caccacttga agaacaacta accattccta atgataagat
     6301 tatcgaaaaa aacaatatta aaattttttt catgataaac ctccaagttt tttaaactct
     6361 gtcttaaatt agtttactac ataattatat aaaaataaat gtatttaatg ttacagtttt
     6421 gattaataat gtaatatttt gataataata cctattgaga ggtgattaga aatgagaata
     6481 ccaaaacatg taggaattat accggatgga aatagaaggt gggcaagtca aaatggtatg
     6541 acaaaagata aaggatatga aagtggaata gaccctggat tagagtctta tagattatgt
     6601 agagatttag gctgtgaaga ggtgactttt tatggattta caactgataa tacaagacgt
     6661 ccatcagtgc agacaaaagc ttttacaaaa gcatgtattg atgctgcaaa tatattaata
     6721 gatgaaggtg ctgctttatt agttgtagga aatacaaaat ctccaatgtt tccagaagaa
     6781 ttattaccat atactaatag aattaaagcc aaagaaggtc aaataaaagc aaatcttctg
     6841 gtaaattatg ggtggtattg ggacttaaat aatatatcag gagtagaaaa tgtaaataaa
     6901 aataatatac aggattacat acagtcattt gatgtatcca gactagattt aataatcaga
     6961 tggggtggaa gaaggagatt aagtggtttc cttcctgtac aatcaattta tgcagatttt
     7021 tatgttatag ataattattg gccagatttt aagagtgacg atataaagga cgcatttagt
     7081 tggtatgata cacaagatgt cactttaggt ggataattaa aaatattctg atttaaggtt
     7141 gtagagtgtt ttaaattatt gaaaaaaaaa cttcttgatg gtaaaataac actaataata
     7201 tgtaaaagtg taaaaattta taccaagaag ggtgaagaaa tgtcaaaagg caataataac
     7261 aataattcaa gaaacaaatc taaaaagacc agtcatctga atagaaaaaa gcgaaaatta
     7321 aataagaaaa agttagcagt gttgatttgc tttacagttt tatttttatt tattgcattt
     7381 aaggctactc aaggtgtagt agctttggta aaaagtatgg ataaaagtaa taaaactagt
     7441 caacaacaaa aagttaactc agaacaattt gaccttggta atgaagagga aaataaaaag
     7501 aaaaaatata ctgtatttat agacccaggt catggaggca atgataaagg aactgaaagc
     7561 aagaccagta atagatatga aaaagaccta aatctacaaa ttgcaaaaaa attagcaaat
     7621 aaattatcta aacaaaagga tattcaagta gtagtatcca gaactgatga tacatatata
     7681 tcattaaagg atagagctat cttagcaaat aatagttctg ctgatgtgct tgtttctatt
     7741 catttaaatg cagagaaaaa tggtaataca gcaacaggta tagaaacttg gtataggaat
     7801 aaagctacag atggttcaaa agaacttgca cagacagtac aaagtactat agtatcctat
     7861 gttaaagtta gagatagggg aatagttgaa aataactttg aagtacttag agaatcaaat
     7921 atgcctgcta tattaataga gtgtggtttt ttaacaacac cttcagagga acaaaaaata
     7981 ataaatgaaa aatatcaaga ccaacttgcg gaaggaattg ttcaaggtgt actttcatac
     8041 ctagattcta aagggaataa gtaagttaac tttactttaa tgtgaggata aatttaaaag
     8101 tttaactgtg taaattgtat taactttatt tcgtaatgtt ggaaacattg tttgaattta
     8161 gaaactatga aattagaaat tattttatct atatctaatt tcatagtttt ttttgcaaaa
     8221 aatttatagt tataaaattt aaaaaaataa atttagtata atgtaatgta gttatttata
     8281 tgtgatttta acttgcattg atttgctatc aattgtgctt tataattagt atatataaag
     8341 tttaactgta agataattta aaaaataggg gaatttatat gaataaaaat agcatgacaa
     8401 aaatttacag tttaaataaa ataattttta ttataattgg aattttagga atag
//
LOCUS       DAMJVX010000150         8426 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMJVX010000150 DAMJVX010000000
VERSION     DAMJVX010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.155"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     regulatory      67..152
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            276..6239
                     /locus_tag="PC407_002927"
     CDS             276..6239
                     /locus_tag="PC407_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HDF2498353.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGN
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNIGTDNIEYTIK
                     KLSASRSEIKNKDENVSYYNIVFNNLPVGKYNIEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDEDGNVQQ
                     IPYENKEYMKNLKDKEQQEEVNNEQEEVNQEEVNNKDENKNQTDNNSNNKDENNDKQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEGDNISKDDNSTTSTYSRAALE
                     TYTDVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSLKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESIDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEIRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIDRELKLAQDLYNDRATLTDVTTVNQ
                     EINKSGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLKTGKNVIQLSKLHSLDIECGGSLYIRYPEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNIGNSY
                     EYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKAKFTDSISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDVSTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPEAETRKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDDKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADQTRTNGIGKLAEDYVYQSDDPSTSEDETGKIPAGS
                     IIITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNAIDVSNKKVITVSNKVKGNIVRDK"
     gene            6449..8329
                     /locus_tag="PC407_002928"
     CDS             6449..8329
                     /locus_tag="PC407_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HDF2498354.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSVTCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTSDKPALK
                     IVTYNNKTVDYENINSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKITKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSNSTSNVTVKKLKGADRFETA
                     TKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAVQFIKSNKVSNIYFIGGI
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVVDKIKSLI
                     EK"
BASE COUNT         3597 a          972 c         1565 g         2292 t
ORIGIN      
        1 taatttaatg taaaaaaatt gtattaaaaa gtttaaatat agtataaaat gggtaagaaa
       61 gtagtgaaaa ttaaatggca aaattaggga aatctaatga cgcaaaacta tagggattaa
      121 agcttttaaa gccatatcag ccagttgcta aaaagagtaa agtcttttta ttttataatt
      181 aagattctct ttgagtgtct ttttttattg tctgaaactt atagtattta aattcatgaa
      241 aaattagaga tttattaagg gaggattgtt gtttcatgaa aaaggcaata tcttgtgtac
      301 tagcagtatc tatgtgtagt gcaccactaa atgtttttgc agaacctata ttagagggga
      361 agctaagggc agttgaaaaa tcagttacag aaaagttaag ggggaatctt gaagtagatt
      421 taaatttttc actgccaata aaaaatagcg agtcagaaag tatgactaat atgcagttac
      481 gattaaaaga tgatagtaac aatagtggaa taataaagct tggagaaaaa gattcaggaa
      541 cagtaaatat tgggacagat aatatagaat atactataaa aaaattatct gcaagtagaa
      601 gtgaaataaa aaataaagat gagaatgtat catattataa tattgtattt aataatttac
      661 ctgttggaaa atacaatata gaagtctctg gagctggatt taagaacaaa gaaattaaag
      721 acattgatat atcaagttat tcacaaagag tcttattaag caatagagca agtgtagata
      781 ataaaaataa acctttaaat tatgaattat tcttaatggg tgatgtaaat ggagacggta
      841 aagtagataa gagtgattat aataaggttt tagagaatat agattcaaat aaaagagaat
      901 ttgatttaaa tagagatgga aaggtagata ttgttgactt agattatgta cagaaaaatt
      961 taggacaaag tgaagatgca aaaagcctag aaagtatagt atctaccaat cctatagtag
     1021 atacaagtaa ggttgaatta aaaggaaata gtgatgtaga tattaatggt aatgtagaaa
     1081 atttatttag tggagaaaat aatggagtta caatatcttc aaaaaatgat gaaatatctg
     1141 aggaaaaacc agcagtaatg gatatagctt tatcagaagc acttaatatg gaagtcatca
     1201 atataaaagc tccagaaggt actgctccag caaaaggaat agttgaggtt actgacgaag
     1261 atggtaatgt tcaacagatt ccttatgaaa ataaagaata tatgaaaaac ttaaaagata
     1321 aggagcaaca agaggaagta aataacgaac aagaagaagt aaatcaagaa gaagtaaata
     1381 ataaagatga aaataaaaat cagactgata ataattcaaa taataaagat gaaaataatg
     1441 ataaacaaca ggaaaatagc gataaacaag aagtaaactc taataccaat caagataaaa
     1501 ataatgataa agaaaattca tcaatggata aacctaattc agatgaagat actccaaaag
     1561 aaggggataa tatcagtaaa gatgataact ctacaacatc tacatatagt agggcagcat
     1621 tagaaacata tacagatgtt caaaatagcg atatagtaat aaatcttggt aaacaagtag
     1681 cagtaaaaaa gataactata aaaataacag ctacaacagc aaacgataga aatcttgcag
     1741 aaatatcaaa agttgaattt ttaaacaatg tatataaaga aataccaaag ccagagatga
     1801 acatacctaa aataaataaa atatcgacta gcactgctgt tggaaatgaa aaagtatctt
     1861 tttattggaa taatgaagta aatgtatcag catacgaact tatactagat aaaattgata
     1921 gtaagggaaa agtattatct actaaaaaat tacaaactag taaaaataca atagaagtta
     1981 gagatataga agcatatgga ctatataggg ttagcataca atctttaaat ggagattggt
     2041 ctagtggtta taaagatgca acgccagaaa acttagatgg agtaccagaa aatgtagact
     2101 caaactataa acctatacaa tttagcccag atagcatatt agaatttcaa gttgttccag
     2161 acacaaaacc agaaccacca gaaggtataa atgttaaagg taaatttaaa tctcttgatg
     2221 taagctggaa aaatcataaa caagctaaag actttgactt gtactataaa gaaaaaggct
     2281 ctaatgggtc ttggataaaa tacaatgata agcttattcg tgggacaagt ttaaaaatag
     2341 aaggtcttaa ggataacaca acatatgaag ttagaatgac agctactaac cacttgggaa
     2401 ctagtggaat gtctaaaaca tatataggta caactgaaag catagatgca ccaacaacac
     2461 ctaactatag attaattaat actcctaagg agaatggtgg atttggaata ccaacaaatc
     2521 acattgtaga tgttgtttat ccagcaggat atgacaaaag tgaatatagt gataaaaatc
     2581 catttgataa attcaatgta gtagatggag acttcacaac acattggaca ttcccaacat
     2641 ataatgcagc tgagggaacg aatagaggac caataataac ttttgataaa tcttatacta
     2701 tggatacttt tatggctaca cctcgttttg atgtttctga taagacaata aatgactttg
     2761 agatacgata ttgggatgaa aatggtcaat tacacaatct gggggatgtt ccttttgaaa
     2821 caaagacaga ccctgtaaat aataataagt atgtaatggt tagattaaaa gaaccaataa
     2881 ctgctactaa gatacaagta agtgtttgtt catcttattc atataagtct aatatatcag
     2941 aacttaagtt ttatgagtat gacagtatag aagatgatgt aagaaatcta tttatggatg
     3001 acctacaagt tgaattaaaa gaagatgtaa ctcaagaaaa aataactgaa ttaaaagata
     3061 gattaaatac tccagataaa gctagtggag aatatcatcc atataagaca gttattgata
     3121 gagaattaaa attagctcaa gacttatata atgatagagc cacacttact gatgtaacta
     3181 ctgtaaatca agaaataaat aaatcaggaa aatataaaga taaaaacgga cagtatactg
     3241 taaattccaa taacttaggc atgcaaaatg actggcaggc attaggagta gctgctagag
     3301 caggagatga aataacagta tatgttggaa gtaaaagtgg aaaaactcct aagttgatat
     3361 atacacagta ctatggagaa tctggtgcat ataagagtgg agaaataaat cttaaaactg
     3421 gaaaaaatgt aattcaatta agcaaacttc atagtttaga tatagaatgt ggaggaagtt
     3481 tatacataag ataccctgaa gatactccaa gtggaggaga tataaaagta cgtgtagcag
     3541 gagctactaa gataccacat cttaatctaa atggaacaat aaatgataag agtcctgcgg
     3601 gagtttctga atctaagaaa aagataaaag catatataga agaattagct aaatataaag
     3661 atgctgtaaa aggagaacca tattatccag gaacaaatat tggtaattca tatgaatatt
     3721 cagaaaaaac tggtgtgtta aacactacag atattgaaag tgataaagta acattaaatg
     3781 ttcctgcaac agctgtatat caaggaatat caaaaggaaa tgctgattta aatacacaag
     3841 tagatagatt atataatagt atgcttgcat gggaacaaat aatggattta gtttactcag
     3901 aaagaggagt atttaaaaca caagatttag atggtaatgg aactgtagat gataatgaat
     3961 ttaacttaac taaaaatgat atggctccaa aatctcgtat gaacataaaa taccaaagaa
     4021 tgtttattgg agcatttatg tatgcttctg gattacatgt aggagtagaa tatggttcag
     4081 ttccagggct tatgaatgga gtacctttcc aaatagatga tagtggaaaa gctacaggag
     4141 gaaacttatt tggatgggga ataggtcatg aaataggaca tgttacagat attggaaaga
     4201 tgacatatag cgaaactagt aacaatgtac ttgctttact tgctcagacc tttgatgata
     4261 agacacattc aagacttgaa ggttcaacaa tggataagat atatgagcat gtaacttcaa
     4321 atagcttagg tataccaagc aatgtatttg aaaggcttgg aatgttatgg caattacatc
     4381 tagcttatga tgatgatttt acgggttcaa tgttaaagaa taactcagat gctgatttaa
     4441 gtaatgatac attctatgca aagatttcaa gaaaatacag agcactttca agtagtgacc
     4501 caattaatag tttaccaaaa gaccaaatgc tagtagcaat ggcatcaagt gttgttgaaa
     4561 aagatttaag agattacttt aaggcatggg gagtagaaat tacaccagaa ttaaacagta
     4621 taatggatag taagaactat gaaaaagaat caagaaagat acaatatgta aatgacgaag
     4681 ctagaagaaa gattttaaat gaaaatatag ctagtatggc aaaagatact aaagtaagtg
     4741 ctagtttttc tgatggaata caaagtggtt ctctagttaa atctaataaa atatctatag
     4801 acctagatgt agataaggat aaagataaga tactaggata cgaaataata agaagtgatg
     4861 gaaattattt agatggtgaa aatggaacac aggtaaaata cagaacagtt ggatttgtgg
     4921 atgcaaaagg aaatagtaag gccaaattta ctgatagtat atctcctctt aataacagag
     4981 catttacata taaggtagtt gcatatgatt atcacctaaa tccaacagaa gaatttgaag
     5041 ttggcactgt aaaattatca gatgaaggga aaataaataa atcagcatgg tcattcataa
     5101 caaatactgt ttctgacggt gatgttagaa ctgaaaatga ctcacatgga ccattacaaa
     5161 atcctgaaat tgataatata aaagataatg atgtaagtac tacatataaa ggaaagataa
     5221 taagtaaagc agagtggaac aaagaccctc aaaaagaccc tgatataaat gtagaagaaa
     5281 atccatatat aatagtagac atgaaagaaa cattacctat agtaggacta aaatatacaa
     5341 aaccagaggc agaaactagg aagttctcat taaaaggatt atttaacttt agaaagaata
     5401 ctagcacaac atacaatcct ttaactaatt acaagataca agtaagtaat gataaaaaga
     5461 actgggaaga tgtaagtcaa ggaacttttg aatatggaca agatttagtt ggtggaggaa
     5521 aagaccaaga taatgaagct agagttacat ttaataaaga tgataaactt tggacatacc
     5581 aagctagata tgtaaaacta atctcaaaca ataagtctaa tatagaagtt gcagaattag
     5641 atataatagg acctccaggt gataatatag aaattggcac aatgaataat gcagaccaaa
     5701 ctcgtactaa tggaattgga aagttagcag aagattatgt atatcaaagt gatgacccta
     5761 gcacaagtga agatgaaaca ggtaagattc cagcaggttc aataattatt acaggtgaat
     5821 acagtggaaa tccagcattt aatttaccac tacttataga taaaaacaat aagactatat
     5881 ctggagaagc acttttattt gcagaagttc cagaaaaagg tgaattaggt gaaatcagta
     5941 aaggtacatg ggtttactat atgccaaaag aaaactttga ttctttaagt gataaggtta
     6001 aggctgaact atatagatac aatgaccttc aaggagatac gccagtaggt caaagatttg
     6061 taagtgatac aatgtatgta cctgtaggag ctaagaatta tgatgagtta aagacaataa
     6121 gtcttactga tagtaatagt aaagctagaa aagctacaag aaatgctatt gatgtcagta
     6181 ataagaaggt tataactgta agcaataaag taaagggtaa tattgttaga gataaatagt
     6241 aggtggaact ctaaaaacgt aaaaatacta atactaacat tgtatatcta atatgattag
     6301 gtactagaat agttttaaaa tagtagatat taaaagcaaa gtatataaat gtaactataa
     6361 gtttagggta atttataaat taaatataaa agctaggtgg ataagtaaat ttatccactt
     6421 agttaatgat agataaggac gggtgttaat gagtaaaaga aaatctttta taagaactat
     6481 agcagtatca actatggcag tagctgtaac tggtagtgtt acatgtgcat atgcagctcc
     6541 agttttacaa ggaacaaaaa cgtatgagaa agtaaatact atagatatat ctgtggactc
     6601 agtagaaaac atagtatatt catttcaagc cagtataaaa gtacaaggtg aggttgaagt
     6661 agtagataat gagcagaaag aaaagattac ttggtctgat aatataaaat ctcagataaa
     6721 aagtggaaat gcaaatgcta cttgtagagt agaatacaac aaatcaagta atacaactac
     6781 attagatata tatgttacat caaatgaaga tttactagac ggaaatagat tgaatatagg
     6841 aagaatatct gtaaaaaaat ctggcagtaa ctcaaatgct gactataaag ttttaggaaa
     6901 aggaacaagc gataaaccag ctttaaaaat tgtaacatat aacaacaaaa cagtggatta
     6961 tgaaaatata aactctgatg aaggattaat atttaccctt attaatgaga gtgaagtaaa
     7021 accaattggt ggaactggtt ctagtaaaaa tgaccctgag aagtataagg tagagaaaag
     7081 tgaagcactt gaatatttat taaacaatat taggataaac tattctattg ttagtaaaga
     7141 aacacaagaa agtggttcaa atgtaatatt aaaacttgga ttagcacaaa aaataactaa
     7201 gggaagaaaa gctacaataa ataaatatgt tgaagttaca cttccaaaaa gtttagaata
     7261 catagttgaa aatgaattat caaaaccgga tgaattacca ccagataatg gcaatggtgg
     7321 aaataatggt ggtggaagta attcaggcgg ctcgtctagt ggtggaagtt ctggaggagg
     7381 aagttcaagt aattctacat ctaatgtcac agtcaaaaaa ttaaaaggtg ctgatagatt
     7441 tgagacagct accaaaatat ctcaaagtgg ttggacaaaa tcagatacag ttgtaatagt
     7501 aaatggagaa gataaaagca tggtggatgg gcttacagct acaccacttg ctagtgtaaa
     7561 aaactcaccg attcttttat catcaaatga aaagcttcct caaaaaactg tagaagaatt
     7621 gaaaaggcta aatccatcaa aagtaattgt gataggtgga aacaactcaa tgccaaattc
     7681 agttgttgaa gctatcaaag ctgtaaattc aaaaatatca gtacagagaa taggtggaga
     7741 tacaagatat caaacttcta ttaatatagc aaaagaaata gataagacga ataatgtaag
     7801 caaattgtat ataggggcag gaaatggaga agcagattca ttatcaatag catcactggc
     7861 cggtaaagaa aaaactccaa tagtacttac acaaaaagat ggtgtagata atgaagctgt
     7921 acaatttatt aaatcaaata aggtgtcaaa tatatacttt attggtggaa tagaaaaaat
     7981 atctaacaag gctattgaac aagttggaaa aatagcaaac aaagatatat caaacaatag
     8041 ggtggcaggt caaactagac aagagacgaa tgccaaagta atagataaat tctactctca
     8101 atctaaacta gatggagttg tagttgcaaa tcaagataaa cttatagatg ctttagcagt
     8161 tggaccatta gcagctaaaa acaattcacc agttatactt gctacaaata cgttagataa
     8221 atcacaagaa tcatcattaa aaggtaaaaa ttcatcaaag ttgtttgaag taggtggagg
     8281 aatagcctca tctgtagtag ataagattaa gagtttaatt gaaaaataaa tattaagtaa
     8341 atcgaaaata agaatgtatt tagatataaa gttatatatt tagatgcatt cttattttat
     8401 tttcattatc ttaacaattt aataat
//
LOCUS       DAMJVX010000151         8307 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMJVX010000151 DAMJVX010000000
VERSION     DAMJVX010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8307
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.156"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            188..1195
                     /gene="asrA"
                     /locus_tag="PC407_002929"
     CDS             188..1195
                     /gene="asrA"
                     /locus_tag="PC407_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HDF2498355.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEIIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            1196..1987
                     /gene="asrB"
                     /locus_tag="PC407_002930"
     CDS             1196..1987
                     /gene="asrB"
                     /locus_tag="PC407_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HDF2498356.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            2000..2971
                     /gene="asrC"
                     /locus_tag="PC407_002931"
     CDS             2000..2971
                     /gene="asrC"
                     /locus_tag="PC407_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HDF2498357.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            3028..3804
                     /locus_tag="PC407_002932"
     CDS             3028..3804
                     /locus_tag="PC407_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2498358.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGLIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            4075..6093
                     /locus_tag="PC407_002933"
     CDS             4075..6093
                     /locus_tag="PC407_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2498359.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLIMERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            6308..7165
                     /locus_tag="PC407_002934"
     CDS             6308..7165
                     /locus_tag="PC407_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2498360.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            7262..8131
                     /locus_tag="PC407_002935"
     CDS             7262..8131
                     /locus_tag="PC407_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2498361.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT         3390 a          747 c         1502 g         2668 t
ORIGIN      
        1 gtgattagat ttgaaatcga ataatataga taataaaaaa atattaacag agattagtaa
       61 actctattaa tattttttta ttatttttga aactgtgata attatcacag aagcatagaa
      121 aataaagtgt taagatttaa acaagatagc aagtaagtaa aactaaaata taagaaaggt
      181 gataaaaatg aaactaaaat tagattcatc taagttcaat gaaggtctga attatctaaa
      241 aaaagactac aagatatttg caccagtttt aatcccattt aaaggtactt tttcagatac
      301 tgatgttata agatacaagg aggtaaacgc ttttgaagaa atggaatttg ctaagaaagc
      361 aaacttttca ccaaaagaag ttgtacttcc cataaatcaa gtattgtttt attttactga
      421 aaaagagttt aaggaaagcg atttagacaa caagaaaatt ttaattttct taagagcttg
      481 tgacataaat ggtataaaaa gacttgatga aatatatcta aataatggtg aagaaaaaga
      541 ttatttttat aaaaatatca gagataaagt aaagtttgca cttgtagggt gtaaggaaag
      601 ttttagaaac tgtttttgtg tgagcatgga ttctaacaaa actgataatt attcaattgg
      661 attaaatata gaaggagata cactgtactt agatataaag gatagtgaat ttgaagtatt
      721 taatggagaa gcaactgaat ttaatgttga ttatgtaact gataatttaa tatctgtaga
      781 tgtgccagag aatattgatt caaatgaaat tataggaaat tcaatatggg atgaatatga
      841 tacaagatgt ataggatgtg gtagatgtaa ttttgtatgt ccaacatgtt catgttttac
      901 aatgcaggat attttctata aggaaaacga aaatgtaggt gagagaagaa gggtttgggc
      961 ttcttgtcaa gtagatggat atacagatat agcaggtgga aattctttta gaaaaaaaca
     1021 aggtgaaaga atgagattta aaactatgca taaaatacat gattttaaaa aaagattcgg
     1081 atatcatatg tgtgttggtt gtggaagatg tgatgatgcc tgtccgcaat acatatcatt
     1141 ttcaaagtgt atagaaaaaa taaatgattt agtaattagt aaggaggaag tataaatgaa
     1201 cccatatata ccagtaagtg caaaaatact agatatagtt aaacatactg ataaagaatg
     1261 gacttttaga gtaaactgca atacaaaagg tgtattgcct ggaaagtttt atgagatatc
     1321 tatacctaaa tacggtgaaa gtccaatatc agtatcagga tatggagagg attatattga
     1381 ctttacaata agaaatgtag gaaaagtaac tagcgagttg tttaattata aagaaggaga
     1441 ttcttttttt ataagaggtc cttatggaaa tggatttgat gtatcccttt atgaaggaag
     1501 agaaattgta gtagtggcag gaggaagtgc attagcccct gtaagaggaa tagttgaata
     1561 tttttataat aataaagaaa aatgcgaaaa gtttaaatta atagtaggat ttaaatcacc
     1621 aaaagatatt ttatttgcag atgatttaaa gagatggagt aaaaaacttg atattttagt
     1681 tactgtagat ggagcagagg aaggatatag tggtaatgta ggattagtaa caaaatacat
     1741 accagaattg gatatgaaag atataaacaa tacttctata gttgtagttg gacctccaat
     1801 gatgatgaaa tttacagtag aagaattttt aaaaagagat ttagatgaaa agaacatttg
     1861 ggtttcttat gaaagaaaaa tgtgttgtgg aataggtaaa tgtggacatt gtaaaatgga
     1921 tgatacttac atatgtttag atggaccagt atttgattat tcatatgcta aaaatcttat
     1981 tgattagggg gcgaagtttg tgataagaga tttaaatacc aaaaaagtaa tgaaaaatgc
     2041 ttatagaatt acaaaaacaa aatatgagac ttctttaaga gttagagtac ctggtggtct
     2101 tattgaccct gagagtttag ctgtagtttc taaaattgca agtgagtacg gaaatggaca
     2161 ggtgcacata actacaagac aggggtttga aatattagga ataaatatgg aagatatgga
     2221 agaaataaat aaaattatac aacctgttat tgaaaaaatg aatataaatc aaagtgagaa
     2281 aaacgcaggt tatccagcag caggtacaag aaatatagca gcttgtatag gaaataaagt
     2341 ctgtccaaag gctcaatata atacaactga atttgctaaa agaatagaaa aagctatatt
     2401 tccaaatgat ttacatttta aagtggcttt aactggttgt ccaaatgatt gtataaaagc
     2461 tagaatgaat gactttggaa ttattggtat ggctttacca atatatgaaa aagatagatg
     2521 tgtaaattgt ggagcatgtg taaaaaaatg ttctaaaata tctgttggag cattaaaaac
     2581 agaaaataat aaggtggtaa gagataaaga taagtgtata ggatgtggag agtgtgtact
     2641 aaattgccca acaaatgcat ggactagaga tgagaaaaaa tactatagat tagctataat
     2701 gggtagaaca ggaaagaaaa accctagact agcagaagat tttcttttat gggtagatga
     2761 ggacagcata ataaagataa tacttaatac ttatagatat gtagaaaagt atatagataa
     2821 agatgcacca ggaggaaaag aacatatagg atatataatt gatagaacag gatttatgga
     2881 atttaaaaaa tgggcacttg aaggtgtaga gcttccagaa ataactaaaa tgtacgaaaa
     2941 catatactgg agtggaatta aatatctata aattgagtaa aacatatatt tgaataaatt
     3001 aaaatttaat agaaggaaga gggaattatg cataaagaaa ctttggataa attaactaat
     3061 gcagctataa ataagataaa tttattaaac acaagtaaag taaaatattt ggtatcttct
     3121 gcgtttgcag gactttatgt aggtataggt atacttttga tatttactat aggaggactt
     3181 ttgactgatg caggttcacc tatgactaag atagtaatgg ggttatcatt tgcaatagca
     3241 ctaagtttgg ttataatgac gggaacagag ttatttactg gaaacaatat ggttatgtct
     3301 gctggtatgt taaataaagg tgtaagtata aaagatactt ctaaaatatg ggcatacagt
     3361 tgggtaggta atttaatcgg agctttggtt ttaggtctta tatttgtagg aactggtcta
     3421 gtagacaaag gacctgtagc tgaatttttt gcaaatacag cagctagtaa agcatccatg
     3481 ccatttacag ctcttttctt tagagggatt ttatgtaata ttctagtatg tgtatcagtg
     3541 ttgtgttcgt ttagaactaa tagtgatact gcaaaaataa ttatgatatt cttatgttta
     3601 tttgctttta taacatcagg ttttgagcat agtgtagcaa atatgacaat ttatagtgtt
     3661 tcattatttt caccaacaat aagtacagtt actattggag gagctattta taatttagta
     3721 gctgtaacac ttggaaatat tgttggagga gctttattta tgggtcttgg aacttatata
     3781 ttaggtaaag aaaagcttaa ttaaatttat tgtataaatt ataactctct tgataaatac
     3841 atttttagta ttattgagag agttattttt atatttttga aaaaatattt gaaacaaaaa
     3901 aaatatggga gcatacgaaa atatatcggt tatatataaa aaaaatacgt ttatatatga
     3961 attatatagt tctaaatata aaaaaagtta aagtcctacc gtaaaaaagc gcattttgaa
     4021 taagataaca aatatgataa aattggtctt gttgaaattt ttagggggga tatgatgaca
     4081 aagaaactag taatatcaaa aacaaagttg tttattttta taatagcatt tatttctata
     4141 tttatatatt tatttggaag taaaaataca cttataggtg taggtatagt aacagctatg
     4201 ctaacactct tggaaagaga tttaactatt tctccaatta aaaatttact gaagtattta
     4261 gcaataaata taatcttagg aatactatca ttttttgcag tacaaaatat gtatttagga
     4321 gttttattaa attttatagc attatttata atagggtatg tatttagtta tgacttaaag
     4381 agagcagtat atgtaccttt tggtctaatg tatatattta tggtaagtat acctgtagga
     4441 aaaagtgaat ttcctatgag attgtcggct ttagctgttg gagcagttat aataatgatt
     4501 gctcaatttg ttatgaatag aaataggatg aaaaatgtcg gagataaaga actgatatct
     4561 atatgtgatg aattattaga aaaaattagt cttttaaaaa atactattaa tgatgatagt
     4621 tctataatta aatctagtat gagaaaaata gatagctgta attatagaat aaattctata
     4681 tctaaaaatt tgaaaatggt tatttttgat aatagaaaag atgactttta tatcagtatt
     4741 agaggtatag acataatgaa tattttattt tcattagaaa gaataagttt aatcatggaa
     4801 agatacaaaa aggatagtaa agaatttgaa gatgaagata taaaaaatat tttagaagaa
     4861 agtagcataa atagtaaatc agatgttcta gttgtagcaa cgaaagaaat aaactatatt
     4921 aagatgtgtt tagaaaataa agataccata actgataaag agatattagg atttagagat
     4981 tatgttattg ctcaggatac taaaaatata aatttaaaag aaatttatag tgttttagaa
     5041 aatttatatg agtttttagt agattataaa aaagtaaaat caagagacga aaagaaagct
     5101 gaaagaaaat caaagatacc tcatgaattt aaacgactat caatatataa gaaaaacttt
     5161 aatttaaatt ctataagatt ttcttatgca gtaaagattg ctttagctac agctgtagcg
     5221 ggttttatta tggattattt ccatttaaga gatggaagat ggataatgtt aactgtcttt
     5281 tcattaacac aaccttacgc agaaaattgt atacaaaggt ctagaaaaag aatagaaggt
     5341 acatttatag gagctgtaat atttattgta ttattttcaa taataaaaga tagtacatta
     5401 aggtcgttaa tagttctttt agcagggtat ataaactctt atgtagttga ttatagaaaa
     5461 ttaatggtat gtgtaactgt ttcagctctt ggttcagcag tagttatggg agacccaaat
     5521 gttttaacca taagcaggat ttcatatgta gcattaggag caattattgc attaatagta
     5581 aataagttta tattaccata tgatgctaaa acaggctatc aacatgttat agaaatgtat
     5641 aaaggaatag ttaaaaatat aattgatgaa gtcaataagt ctatagaaaa tgtagctgat
     5701 gtttactata taaaaaattt attattgata ccatcactta ttgaagatag acttatgttg
     5761 ataaatgcta tatataaaga taaacatcaa gaggattttt tagaaaatca gaaattacta
     5821 ataagtaata tgtataatct atatataaat gttaataaaa ataaaataaa agatgaagat
     5881 gttgaaaaaa tattgagaga tactaattat atttcaaatt acaatgtaga taagtatgat
     5941 gaaggaagaa gtgtaatttt agagagtatt gtaaatacta aaagtctagg tgacaagata
     6001 atatgtctaa acttactgca aactctaaat ggtgtcaaag aaatgtatag aataagtaat
     6061 ataactaaag tttcaataaa agaagcagct tagatattaa attatagaat agccatatat
     6121 ttaaaataaa gtatatggct atttgtttta taaaatacta aaagaaaata atatataaaa
     6181 aacaaaaaac atatatttat gaattttata tgaaaatagt taaaatattg atttttttca
     6241 ttggttgaaa aaacaattta cttaggataa actataatag tattcaaata ttatgggggt
     6301 tagatttatg agtaaagatg taaaggtaaa gcgtaataag aaatatgata catttagtat
     6361 aactatggaa cttgagagac ctttacttta tgatgaggtt gagaacaaag atgataaaaa
     6421 taataataga aaatttaaca taaagaataa gatatttaag ggaataggta tactatttgt
     6481 attgattttg cttggctttt tcatatggat aaataaaaca tatgaacctc aaaagctggc
     6541 aaaagaagct ctagtcagca atagtaaagt tgaagtaact gtaaatgaaa acatttcatt
     6601 tacaccaaaa ggaagaaaag tttctaaagg tttaattttg tatccaggag ctaaagtaga
     6661 aatagaatct tatgctccac ttgcaagaaa aatagcagaa agtggatatg aggttgtaat
     6721 cgtaaagatg ccacttaatc tagctatact tggaactaat aaggcacaga aagttatgga
     6781 ttcttacaac aacatagaac attgggtaat aggaggtcat tcattaggag gagttgcagc
     6841 atcaaatttt gcacgtgata ataaattaat tgaaggcgtt gtatttttag cttcttatcc
     6901 aatgggtgat gagttgaaag aattaggaaa aaaagttata tctatatggg ggagcaaaga
     6961 tggagtagtc aattttaaaa gtcttgtaga atcaaaacaa aaacttcctg atgatacaac
     7021 ttatgttgag attgaaggag gaaatcatgc acaatttgga gactatggaa aacaaaaggg
     7081 agataatgat gctattataa gtcaagaaaa acaacttaat attactgcca atagtatcat
     7141 taaattttta aagaatatat cttaaactaa aatttaaaaa atataatttt ataagttatt
     7201 atttaaagta aatattaata atataataga atgaatattt tattacaagg gggattttaa
     7261 tatggaatac gatatgccac tttacagacc accaagtgag gcttatagtc ttataataca
     7321 agtcacacta ggatgttctc ataacaaatg tacgttttgt agtatgtaca aatctaaaaa
     7381 atttaaaata aagccactag aagtaataaa aaatgaaatt gatatattta gaagatatta
     7441 taagcatgta gaaagaatat tcttagcaga tggagatgca ttgataatac caatggagaa
     7501 attaagagat attattttat atataaaaga agtttttcca gagtgtgaaa gaattactat
     7561 gtacggctct cctaaatcta tagagaaaaa atctgatgat gaacttaaag aattaagaaa
     7621 acttggtgtt aaaatgattt atctagggct tgaaagtgga agcaatgaag tattagaaga
     7681 tattaagaaa ggttttagta gtgaacaatt aataaaagtt ggtagaaaag ttaaaaaaag
     7741 tgggattaaa ttatcagcca ctgtaatagc aggtcttgga ggaactaaaa aaacacatca
     7801 gcatgcagtt gatacaggaa aaatgttagg agcaatttca ccagattatg tgggtgtact
     7861 tagtttaatg gtagaaccta atactgaact ttacgattta cttaaggcag gagagtttac
     7921 aatactagag gataaggctg tgttacaaga aattaaagag atgataaaga atatagatgc
     7981 taatgaaaaa attgtattta gaagtaatca tgcatcaaat tatgctaatt taaaaggtgt
     8041 attacctgat gataaacaac gtctaattga tgaaattgat tactatctaa gtaaccaaaa
     8101 gctaaaaaga gaagaatata gaagactata gtttttgata tatcaatcta tattgatagg
     8161 taaatactaa gattatttag atacaaaaaa tcatgctaca ttaatagttg cttactactg
     8221 atgtagcatg atttttttat taaaaaacaa aatggtatat tcatatataa tatatcaata
     8281 ataatgaaaa tgtgaactag tttaata
//
LOCUS       DAMJVX010000152         8289 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMJVX010000152 DAMJVX010000000
VERSION     DAMJVX010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8289
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.157"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            120..1151
                     /locus_tag="PC407_002936"
     CDS             120..1151
                     /locus_tag="PC407_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2498362.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALLMKVADVVRDCL
                     DIEDNNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1284..2582
                     /locus_tag="PC407_002937"
     CDS             1284..2582
                     /locus_tag="PC407_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDF2498363.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(2696..2941)
                     /locus_tag="PC407_002938"
     CDS             complement(2696..2941)
                     /locus_tag="PC407_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2498364.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3089..3955
                     /locus_tag="PC407_002939"
     CDS             3089..3955
                     /locus_tag="PC407_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDF2498365.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(3959..4495)
                     /locus_tag="PC407_002940"
     CDS             complement(3959..4495)
                     /locus_tag="PC407_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HDF2498366.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLAQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(4685..4888)
                     /locus_tag="PC407_002941"
     CDS             complement(4685..4888)
                     /locus_tag="PC407_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2498367.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            5179..5943
                     /locus_tag="PC407_002942"
     CDS             5179..5943
                     /locus_tag="PC407_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2498368.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKYDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKKTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            6332..7645
                     /gene="prdC"
                     /locus_tag="PC407_002943"
     CDS             6332..7645
                     /gene="prdC"
                     /locus_tag="PC407_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HDF2498369.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     gene            7814..7976
                     /locus_tag="PC407_002944"
                     /pseudo
     CDS             7814..7976
                     /locus_tag="PC407_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3329 a          856 c         1399 g         2705 t
ORIGIN      
        1 tatatggtgt acttatttta tgtttttagc aagaaatata gcatttaaaa gataaattgt
       61 gcaacttaaa tcaaagttta agatgaacta ttaacataga tatattaaaa acttttgtaa
      121 tggagaatat atgtgatatg aataataaag ttaaggttgc agtattagat acaggaatag
      181 acaaagaaca tgattactta aaagataatt tagttggagg aattgctttt gaatgtatgc
      241 atgattatat ctttatatca gataaatttg atgatgaaga tggtcatgga actgcttgtg
      301 catcaataat aaagaaagag tatgaagatg tagagttatt tgtaataaaa attttgagaa
      361 aagatggtat tacaaatata aaagttcttg aagaagcatt aagatacctt ttagacacta
      421 acataagatt aattaattta agtttatcag tcataggagt agagagtgtt aagggcttat
      481 ttgaagtttg ttacgaattg tttagaaaag gaaaaataat agtatgttca ctcgctaatg
      541 attttgattt aagttatcca gctatgttta acaatgttat aggtgtaaga ggatttactt
      601 tagatggaga tgattcattt tggtacaaca aaaaatatga tgttcaatgt gttatggata
      661 gcaattcata tataagttgt gatataaata attcctatag attaccacca aaatgtaata
      721 gttatttatc agcaaagctt actggtaaaa tagctaagat attatcagaa aaaccaggca
      781 taacaatatc tgatttaaat gataagcttg aatcattagc aactagaaat cattgggata
      841 gttgtgattt tgatgagtgt agtagaattg taaactttaa attggatttg tatgacaaag
      901 aaaatgctct tcttatgaaa gttgctgacg ttgttaggga ttgtttagat attgaagata
      961 ataatgaaaa actctttaag ggtagccttt ttaataagga gataggtttg gtttatgata
     1021 attgttttaa tctgttgaaa aagctagagc atagatttga cataaagttt aattatatgg
     1081 acatctcaaa atatgactta gtttcaatat atacactgac tgaattagtg gaaagatata
     1141 aaaacttata aataaagatt tgtagctaaa tttaatgtag aaggtattaa attatatgta
     1201 ttctttgtcg gtagattaaa agtaaaaaat atactataat tagatagcat aagagaaaat
     1261 gccaatacag ggggagttag ataatgaaat atgaagaagc attagagtac atatcacaaa
     1321 ctaataaatt cggtataaga ttaggattag aaaatatcgg aaagttatta gaattattag
     1381 gaaatccaca agaaactttg aatataatac atgtagctgg aactaatgga aaaggttctg
     1441 tgtgttcttt tgtttctaat atactaagag agtgtggata taaagttggt ttatatacat
     1501 ctccatattt agaaactttt actgagcgta taagagtaaa tggacaaaat ataccacagg
     1561 atgatgttgc tagaataatt gagttaataa aagaaaaaat agagattatg gtaaaagaag
     1621 gctatgcata tccaacagaa tttgaagtgg ttactgcaat ggcattttat tactattcag
     1681 aacaaaaggt agactttgtg gcattagaag ttggtttagg tggcagatat gatgctacaa
     1741 atataataac aaaatcactt gtaagtgtga tagtatctat aagtttggac catacaggta
     1801 tacttggaga tacaatagaa aaaatagcgt acgaaaaggc tggaataata aaggaaaatg
     1861 gagtagtact agtatatgac cagactgatg aagcgaagga tgttataaag tcagtttgta
     1921 aagagaaaaa agctaaatat atagaagttg attttgatga tataaacata aaaaaatcag
     1981 atataaattc tcaaatatac gattgtactg taatgaaaga aacttataga gaccttgaaa
     2041 taaaattgat aggtgaacat caaataaaca attctatctt ggcaattagt gtaataaaat
     2101 atctaaaaga cataaataaa ttagcaaata taaatgaaga atctataaga aaaggactta
     2161 taaatactaa atggccagga agaatagaaa agataaaaga aaatccaatt tttataatag
     2221 atggtgcaca taatgaagat ggagcaaaat ctttggcaaa agcacttgat aagaatttta
     2281 aaggtagaaa attaacctta ttaataggta tgttagaaga taaagatatt gatagtgttt
     2341 tagagatatt gttgccacat tttaataagg taataacaac aacaccaaat aacccaaggg
     2401 ccataaattc agatatatta agagaaaaag tattaaaata tgtagatgat gtaacatcaa
     2461 agcatgagat agaggatgca gtaaattata cattagaaac atcaagtgag gatgatatta
     2521 taataagtgc aggttcatta tatatgattg gtacagtgag aactttagta aagaaattat
     2581 aattttataa aataaatata caaataaaga tataaaaaag agttgtctaa aaaatggaca
     2641 actcttttta tataaagtaa gtttttaata attttactga tgtttatata agtaattata
     2701 tagattgacg tatagcctta gataattggt ctgggcaaga agtacctttt ccaccacagt
     2761 caataccgtt taatttatca gcaacttcaa gagcattttg gccgattatt aagcttctaa
     2821 gtcctgataa attaccatta catcctccta taaactcagc atcaacaatc acattatttt
     2881 catcaacttc aaaaatcatt tctctacaac atacaccact tggattaaat gttactttca
     2941 ttaaaaagac ctccattaaa tattatttct ttttttaaca ataataataa taccaaatat
     3001 atgcgtaata atgcaactat aaagcataat acaagctaat gaaataatat attattgtag
     3061 tatatgtatt gtagagagga ggggttatat gaaaatctat atttctaact acttatcaaa
     3121 gagtattagc atagttgatt attctacact tgaactagaa aaagaaatag tattagaaga
     3181 taatatatat ccacaccatt tttgcataga aaaagaaaaa aatttaatat acatacctag
     3241 ttcaagtaat gggatacttt atgtcttaga tttaagtaat gataaaataa ttgatactgt
     3301 ctctatagga ggaagtctta gtcaagttgt acttagtgat aatgaattgt ttgtggcaaa
     3361 tgaagattct aatagtatat atgtactaga taaaaacaca ttaaatccaa taggaattat
     3421 tggagttgat aatatgccac atggatttga ctttgataga gaaagtaaaa aattatatgt
     3481 accgtgtata aactcgatag tatgtataga tacaattaat aaaaatatac aaaagaaaat
     3541 aaatatggac tttagagctt ggcatataga gcttgataaa caaaagaaag aaatgtatat
     3601 atcaacactt gatggaaagc ttataataat agatgaagta agcatggata taaaaaaggt
     3661 attatatgaa tttttattac cagtagaaat aagatttaat tatagtggca aaaaagttta
     3721 tgttgctgat ttgggttata ataatgtaag aattttagat tatacaacga gtagatacat
     3781 tggaaatatt gaaataaatg ggattcctca aggcttagaa atatctaaag atgaaaagct
     3841 actatttgta tcagatacac aagaaaattc agtaaaagtt tatgaaactg caaacaataa
     3901 attaataaaa gaaatcaagg taggaaaaga gcctacaact ataatttgtt tgtaggcttt
     3961 aatgtatgtc tcttataagt aaatcgataa cttcggcata catataagat gcattatttt
     4021 tccagttatt gcaaataagt tttgcttgtt tctctgatga aacatttaga tttaaatcta
     4081 ttaagttaga ctgattttct atgactctaa ggttgacaat aaattcatta tcactttgtt
     4141 ttacaaaact agactttact tgagtatctg ctagtagatt ttctttgtta gaatctatat
     4201 acttgtctat tttttcagta acactagtag gtattcgatt aaagaagtat tctagacttt
     4261 ctacgcctct ttgagtaaga gcataatatt ctctgtcaga gtctttatat gtagtcaaaa
     4321 acttagattc cataagctga gctaaaagct gttgtaatga aaaataattc atcatctcag
     4381 tctctaatac tacttgagtt atttgagaat tagttaaatc cattttaatt ttttctaata
     4441 tatataaaat taaaagtttg tgataggcta attcttcaga cgagttttca aacataaata
     4501 tcatcctccg tcaaactatt tctatatata atatattaaa ctaatttgta caaaattaaa
     4561 agaacaattt gtattgtatt attaatcttt aataattcat ataaaacaat tttataaata
     4621 aatatagaga aacagggctg ctaattagca gccctgttga tacccaggtg tcaaaattta
     4681 ctatttattt tccagccatt tgtctttcag ccatttcaac taatttctta gtcatatatc
     4741 caccaacata tccattttct ctagctgtta agttaccttt atctatgttt tcatagttag
     4801 ataaacctat ttcgttagct atttctaatt tcatttggtt taaagcagct tttgcttctg
     4861 gaactactgt tctgtttgaa tttgacatag taaatttcct cctcaaataa ttagtttatg
     4921 atgtctaaca acacttttat ttatgtgatg ttaatactaa tttctccaaa ataaaaataa
     4981 tatatacaga aaaataaagg taaaaatgta aaaaatataa aaaaattaaa taaatgagta
     5041 ttttagagag tttaattagg tcaatatagg ttttattttt ttagttattg tcttataaat
     5101 taaatattac agtacttttt tgagctgaaa aaaaataaat tttaaatata gaagaaatta
     5161 tttcaaaggg ggaatagtat gaaaagagaa gttaaaagga ctaaagcttt gatatatagt
     5221 gttttatttt tttcattatt attagtgtta atatttgttt ttgtttatgg aataaaatac
     5281 atatgttcta aatacgacaa ttcaaaaatg cctatatata aagttgatac aaatgagaaa
     5341 aaaatagcct tgagttttga tgtagcatgg ggaactgata atatggatga tattttaaaa
     5401 attttagata aacataatgt aaaagctacc ttctttttag ttggtagttg ggtggatgat
     5461 aatgaagaaa ttgtaaaagc tatagatgaa aaaggacatg aaattggaaa tcattcaaac
     5521 actcatgcca atttgaagga aatatcaaaa gaagacataa caaaagagat agaaactaca
     5581 tcagaaaaaa tatataatat aacaggaaaa aagactaatt tatttagacc tccttttggg
     5641 gacgtaaaca ataaagctat ggacatttgt agtgatttag gttataaagt aattaagtgg
     5701 gatgtagatt ctatagattg gaaggagctt ggaccaaatc atgtaattga gaaagtaata
     5761 aaagaatctc aacctggttc aatagtttta ttccatgcta atataaatga tgtagataac
     5821 tatttggata ctataataag tcgactaaaa aaagatggat acagtctagt caaaatatct
     5881 gatttgttgt ataaagataa ttatgtagtt gattccaatg gagtgcaaaa aactaagaat
     5941 taataagtaa taaatgtata ttttaagtaa agttgtgata aattatatag tatatgttag
     6001 aaaagtgtcg ataaaaagac ggtgtctatt ttttgacact ttttatacat gtatgcaata
     6061 tcaataatta tagattaaat tgattaaaaa agtgtctaat aattgacttt tttttgatgg
     6121 atttaaaaat ttagaagaat taatgaatgc tgtaagacag tatttccaaa gggtataagt
     6181 ttgtttaaaa gttggcatag aaattgcttt gttaataagc gatatgttct atagaaataa
     6241 acaatatgtt aagaccttat aatagaaaaa tagcattaac attatttgaa cagcttataa
     6301 aaaaattaat atacttaagg gggcgtacta gttgaacaaa ctatattcta ttttattaaa
     6361 acaacacgtt ggtggtccag ataagccagt agtaagtgtt ggagatgtag taaaaaaagg
     6421 aactctaatc gcagaaccaa ctggattagg ggcaaatatt tatgccagtg ttagtggaaa
     6481 aataagtgaa ataaatgacc aagcgatagt aattgaagct gatgaagtac aagaagatac
     6541 ttttgaacca ttaaaaggag aaggtatact tgacttaatt aaagaagctg gagtagtagg
     6601 aatgggtgga gcaggattcc ctactcatgt aaaattaaac atagatttaa atggtggaac
     6661 aatattagca aatgcagctg aatgtgaacc tttattagct cataatataa aagaaataga
     6721 agaaagacca gaaatagttt atcaaggtat aaaatatgct atggaagtta ctaatgctgg
     6781 aaaaggtatg ctagcaataa aaagcaagca tccaaaagca atagaagctt tcaaaaaagt
     6841 aataaagcca ggagacaaca tagaagttgc agaactagtt gatatgtacc caatgggaga
     6901 agagagagca atagtaagag acgttctagg aaaattactt gagccaactc aattaccttc
     6961 agaggctaat gcagtagtaa taaacgtaga aacactaact agaatagtag aggctgttga
     7021 gcaaaaaaga ccagttatat ctaaaaatat aactgtagtt ggtcaattaa acagtggaaa
     7081 agagtctata gtatttgaag atgtgccaat tggaacaact gtcggagaat taattgaaag
     7141 agctggtgga ataaaaggtg aatatggtga aataatccta ggaggacctt tcactggtaa
     7201 agctactact ttagatgctc caataactaa aactagtggt ggtataatag ttactatgcc
     7261 atttgttaat gaaaagagaa aaatgggtct tttagtctgt gcatgtggac ctaatgaaga
     7321 gagaatgaga gatatagcaa ctaaaatggg agttactgat atagtatcag ttcaaaaatg
     7381 taaacaagct caagaaatta aaggtgcatt aaaatgtgaa aatccaggac actgtccagg
     7441 acaagctcaa aaatgtattg aatttaagaa agctggagca gaagttatat taataggaaa
     7501 ctgtactgac tgtagtaaca cagtaatggg ttctgcacca aaattaaagt taggtgtata
     7561 tcatattact gaccatgtaa tgagaacagt taatcaccca ttaataagaa gaataaaagg
     7621 tgacgtaaga ttaaaaataa aataatagat aaatagaagt tagacatctt tagaagtaaa
     7681 acttaaaaat tacttctaag gatgtttttt aatgtatttt gagggcaata tatagcttta
     7741 aaagaaatca aaaaagtgcc aaatgtaaaa ctaattaaac catatatatt taagaaggga
     7801 ttgaattgga gttgtgaatt atgttagtag aaaccattct aagtgtttgt tattggtaca
     7861 tttgatattt gtatgtaaat acagaaaaaa ctttttgctc agatggatac tttgctttca
     7921 gtgtaggaaa tgtttctaaa gaaatgattg aaaaatacat acaaagtaag ggataggttt
     7981 acatccacca acctaaaggt atagtggttt tacgccttaa tttataaaaa gaaattaatt
     8041 tatattatta ttaagtttaa aataaaaagt ctcaaattga tttataagtc attttgggac
     8101 ttttttatgt aaatataata tgcaaatata gtttgtttaa aaatatctat tttaattata
     8161 ttcttaaatt tatgattgga tacaggcatt cattaattgt ttgatgaata gaaaaatgtt
     8221 ttgaaggtga aaatagtatt gaaaaaataa tatactaagg gtataattca tttgatttca
     8281 tttgtataa
//
LOCUS       DAMJVX010000153         8278 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMJVX010000153 DAMJVX010000000
VERSION     DAMJVX010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8278
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.158"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(134..658)
                     /locus_tag="PC407_002945"
     CDS             complement(134..658)
                     /locus_tag="PC407_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2498370.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(723..1475)
                     /locus_tag="PC407_002946"
     CDS             complement(723..1475)
                     /locus_tag="PC407_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2498371.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDISKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1506..2804)
                     /locus_tag="PC407_002947"
     CDS             complement(1506..2804)
                     /locus_tag="PC407_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF2498372.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQQGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREVNVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2821..5085)
                     /locus_tag="PC407_002948"
     CDS             complement(2821..5085)
                     /locus_tag="PC407_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF2498373.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5340..6158)
                     /locus_tag="PC407_002949"
     CDS             complement(5340..6158)
                     /locus_tag="PC407_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2498374.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGIDEKIIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIIL
                     LDEPTAGLDPVSTRSIVDIIKGLNKDNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFVNENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6151..6849)
                     /gene="cbiQ"
                     /locus_tag="PC407_002950"
     CDS             complement(6151..6849)
                     /gene="cbiQ"
                     /locus_tag="PC407_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF2498375.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIKNIFVLSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEIFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKMHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6852..7136)
                     /locus_tag="PC407_002951"
     CDS             complement(6852..7136)
                     /locus_tag="PC407_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2498376.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPDYEPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGVQKGKRS"
     gene            complement(7126..7878)
                     /locus_tag="PC407_002952"
     CDS             complement(7126..7878)
                     /locus_tag="PC407_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF2498377.1"
                     /translation="MKQNIKLGVIAALMLIVLTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLVGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIISFLIFKALKKKDG
                     NNAMPVFLAAAIGDLATYTVTSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8067..8259)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         2584 a         1522 c          895 g         3277 t
ORIGIN      
        1 tccacaatac aaataaatta aaaagtgaaa tgtatttgtc taaaaacata ataacatttc
       61 actttatcct catttttaag taatatatat ttttaatttt tatttataac tcaatctagt
      121 ttttatataa gttttagttt ttttctacat ctgagtaatc tattccaaaa gtttctacag
      181 taacagactt cataatttgt ggagtttgtg gcttatcagc tgaatctgtt ctaactgatg
      241 ctatttcatc aactgtatcc ataccttcta tcactctacc aaaacctgca tactgaccat
      301 ctaagtgtgg tgagttttta tgcattatga aaaattgaga accagctgaa ttaggtgcca
      361 tagttctagc cattgatatg actcctcttt catgttttaa attgttagta aatccattac
      421 cactaaattc accttttatt gagtgacctg ggccacccat tcctgtacca tttgggcatc
      481 ctccttgtat cataaatcct ggtattactc tatgaaatat tattccattg taatatcctt
      541 catttactaa tgatacaaag tttttaactg tatttggagc tatatttgga tataactcaa
      601 ttttgatttc ttttccattt tccatttcta tagttactat aggattttta ttttccataa
      661 tttattacct tccttttcat tagttaaatt acataattac atttattata ttatactcta
      721 aattaaagtc tatcaagtat attttttcct atttcgtatc cttcttgata aatagtttta
      781 caaaactcat catcaaagtt aagcgtatct ttaggagcaa attgagtatt aggtctaact
      841 actattattt tatcttcatc atatactttt ttaatatcct ctggcaactc ataattatat
      901 tttgtggata ttaaaataat tttatccaca ttatccccaa ttaatggttc aattaatgca
      961 ctcctaatta aacctccatc taatttgaaa ccatcataaa ttccttcttg cacatctttc
     1021 acaaattgtt ctttaaatcc aagagttgcg tttggatttg atggtaacaa cccactgtat
     1081 gaaatgctga taatggcttc attacgaggc atttttgata tgtcaacaat cttttctgat
     1141 actactttat tatcaacttc aacataatta acataaaagt tcatttcttc tatattacta
     1201 tcatctagtt cccttaggtt gtctatagca ggagaattat ccacagtatt atttactatt
     1261 tttacatttc cattctggat atcttttata tttgtccaca ttttctccaa gttatcaaca
     1321 ttccctgtgt acaaataata tccatttatt gctcctattg atgtccctga aatcgaataa
     1381 aatttattta ttcctccatc atacaaggct ttaactactc ctgcttgata agcccctttt
     1441 gctcctccac cttctaaaca taatcctaat ttcatagtct catctccaaa ttgtttatct
     1501 tatatttata tttcaaaaaa cttactaaat gttttatata tactatagtg gtcctcaaca
     1561 tttcctaatt ctcttataga gtgcattgct aaaattggac ttcccacatc cacagatggt
     1621 atatctatat gagtagaaga tattggacct atagtacttc ctcctctttc atctgaccta
     1681 ttaacaaatt cttgatactc aacattaact tctctacata tatttttata aatagctata
     1741 gaatatccat cactagtata tgcctgacta gcatttatct ttataactgg accttttccc
     1801 attattggtt tattagttgg gtcatgcttt tctggaatgt ttggatgaat agcatgagct
     1861 aaatctgatg atatcataaa agatgaataa atagactcaa agaatccctc tctatcctta
     1921 cccattgata tgcaaattct ctccaatata tttaacaaca tgtttgaatc tgcaccttgt
     1981 ttagtagagc taccaacttc ttcattgtca aatacagcca ttacatttac accttgttga
     2041 cctttagtat taacaagagc attcaaacta gcatgtacca ttgcaagatt atcttgtctc
     2101 ccaatagata taaattcatc attagcccct attaaacttc ctttttcaaa ttcatataaa
     2161 aataaatcaa aatctataat atcttccata tttacatcta agttagatgc tatttgcttt
     2221 ataagaaaat tatctttttc taatgtatca tttaacatcc caactaaagg taacatatct
     2281 ttttgcttat tatatgaatg accatcattt atacttctat ttaaatgtat tgctagattt
     2341 ggtattatac aaattggctt tttaaagtct ataagccttt catttggttt taatatacta
     2401 tcacctttta aaactactct accagctatc ccaagaggtc tatccaacca tgtacttaat
     2461 attgctcctc catagcattc tgtatttaac ttcaaataag atttttcagc ttctatttct
     2521 gcatttggtt taatactaaa tgtaggagaa tctgaatgag agcctattat tctaaatcca
     2581 tctctttcta tatcttctga atttactaca aaagctacta tagctgatag attcttagta
     2641 acataatact ttccaccaac tcttagattc catctttgat tcaaaactaa ttcttcaaat
     2701 ccatttttat ctaagatttc cttagtactc tcgactgcat gaaaagacga cggactatca
     2761 tatatgaagt ctattaagtt tttagcaaag tttatactat ccattcctca tttacctcca
     2821 ttataaaata tttagtgttg tatattcttt atttattttt ttaattccct gttggtcaaa
     2881 agctatagta acatctgtac caattataga aaccactgtc ccaaccccaa atttaggatg
     2941 atgtacctta gaaccaagtt ttatatcatc tggattagtt tctttattag aatcttttat
     3001 tgtagcatta actttatctg caactttaga tttgcttata gtactcatat actttttagt
     3061 atatttatct aacatattat aacttgcttt agaatagctt agttcttttt ttactttgta
     3121 caatctttct atacattctt ctggcagttc ttccataaat cttgaagcta ttgcaacatt
     3181 tgttttacca tataatgttc tcttctctgt caatgacata tatagctctt ctttagctct
     3241 agttatacca acataacaaa gtcttctctc ttcttctatt tgtgaatcac tcatagattt
     3301 tatagctctt gatataggaa ataacccttc ttccatacct gttaaaaaca caacagggaa
     3361 ctctaaacct ttggatgtat gaattgtcat aagcgaaacc ctatcattgt cttcttcatc
     3421 atttgattca gatgtaagtg ctatacttgt taggaatgtc tctaagtctt tattttcata
     3481 agtatcatca ttactacttt caaactccaa agctatagaa ataaattctc tcaaattatc
     3541 aattctgtct tgagcttctt cacctttgcc tgttaaatct ttttcatttt tatttcttat
     3601 ctctacaagc tcgtccatat atcctgtagt atccaagact ttttctataa gtttgctaac
     3661 aggataaact tcttttatag ttctaagagt ccctataata tcaacaaatc cacttatact
     3721 tgcccttgcc ttagtagata tatctgaatt tgtttctata tcaattaata ctgagtatat
     3781 actttcctgt tttaagttag ctctatcttc aattttttct atagttctaa gccctatacc
     3841 tcttctagga acatttataa tcctttttat agatatatca tcttgtggat tttgaataac
     3901 tcttaaatac gccaacaaat cctttatttc ttttctctcg tagaattttg taccaccata
     3961 tatattgtat ggaatttgac ttctatttaa agcgtcctct acaggtcttg cctgtgcatt
     4021 tgctctataa agtactgcaa aatccttata aggtctattt tcttttctag ctatttgtgc
     4081 aattgaatca gcaatgaaat cagcctcttc tatttcgcta ccagttaatt gtatctttat
     4141 aagttctcct tctttttttt cactccaaag cttttttctt tttctttcta tattattaga
     4201 tataacttta ttagctgcat ctaatatcac ttgtgtagac ctataatttt gttctaactt
     4261 tacaacatgt acattgtcat aatctttctc aaattcaagt atgtttctta tatctgcgcc
     4321 tctccatcca taaatacttt ggtcatcatc tccaacaaca caaatatttt gatgttctct
     4381 tgctaataat tttatcaatt catattgagc tttacttgta tcttggtact catctaccat
     4441 tatgtatcta aatctacttc tgtaatatgc taatacttca tcattagcct tgaaaagttc
     4501 tacagtttta agtattaaat catcaaagtc aagtgcacta tttcttttta aacggtcttg
     4561 atataacgca taaatatctg caatcttaac cattctatta tctgccatgt tgatatcttt
     4621 aaactgcttt ggagtataaa gtttgtcttt tgctcctgaa attgcactta taactgcttt
     4681 tggttcaaac actttatcac ttaaatttaa ttcttttaga cagtctttaa ccaatgtaac
     4741 ttggtctgct gaatcataaa ttacaaaact tctattgtaa ccaatcttat ttatatcttt
     4801 tcttagtata cgaacacagc aagaatggaa tgtacttatc cacatatcct tagtttcagg
     4861 acctatattt tgttcaactc tctctctcat ttcatttgca gctttgtttg taaatgttat
     4921 agctaatata tttggtgctt gtacaccttt atcttctata agatatgcta ttcttgttgt
     4981 taatacctta gttttacctg aaccagcacc agccagtata agtactggac cttcagtctt
     5041 ttctactgct tctctttgag caggatttaa tgtatctaaa ttcatatttc tcctcctgtt
     5101 aacagccata aatatatttt aatctaaaat atgaattact tcaaattata attatgtatt
     5161 atattaatag attatttcca atatattaca attaaataac atttttccat ataatttatt
     5221 taattgttta ataaaaaaaa taaccttatg atttggggaa tcataaggca cctttaaggg
     5281 gtctaggtta agggttatta tatataaatc tattttttta aaataggttt agggcttaat
     5341 tatttatttg tattaagttc tctttccttc caatacttat acaaatcttc ttctttctta
     5401 aataagggta aattcatatt tctatgtact tttactaacc atggggattc taggccagct
     5461 tgtataatgt tattttcatt tacaaataca ttttctgcct ttccttcatc aattaaaatc
     5521 cccttatcca atacatatat ataatcgcat atttcataca ttaaattcat atcatgactg
     5581 gatattacta tttttatatt atctttattc aatcccttta ttatatctac aatagaccta
     5641 gtacttactg ggtctaaacc agctgtaggc tcatcaagca atataatttc attttccatt
     5701 gctataactg atgctatcgc tacccttttt ttctgaccat aacttaaaaa atgtactggt
     5761 ctatctataa aatctattcc attaacagct actagagctt tgtttattct ctctttaatt
     5821 attttttcat ctataccaat atttctcatt gcaaaggcta tatcatcata tacttttgaa
     5881 taaaaaattt gtttttctgg gtcttggaac acaataccta cattttttct aaggttgtat
     5941 aaacttctct tatcatattt taacttttct tctttaaaaa gaatttctcc agatgtaggc
     6001 tttaatatac ccattaaatt cataaataat gttgattttc ctgaaccatt tgaacctatt
     6061 atacctataa catttccctt tgaaaaatcc atattaatat ttaatagtgc attggtattc
     6121 ttttcatatt gataagttaa attatttatt ttaaacatca ttatccccta ctatgtgaaa
     6181 tttaccatca tacaatttta tgtctaatga tatacacata tcatcatacc ttgtcataag
     6241 tcttttgtac agcatactgc caagaattcc taatgattta tatgaagttt ttaaatttat
     6301 gtacccaaat cttagttctt gtgacttata tatttctgaa aattcttcta aaaatatgaa
     6361 tataaaccta tatattaaca ttgatagctc aacaaaagta tcaggcaaat gcattttttt
     6421 aaagaagaat attaattgat taaaaggtgt tgtaagtata caaaagtata cacaagtcaa
     6481 acaagacata gctctaaata acacatgaat agacatgtca atagatgctt ttgatattcc
     6541 tacattgaaa ctaaaaattt caaaactata taataagtct gccttattaa aagatatatt
     6601 tacaagagtt attcctatac taagaaataa aaagtacatt ggtattctga gtaattttaa
     6661 ataattcttt aaatctattc cagctacaca aacaactaga atactcatta aaatcataat
     6721 tccacttaaa acaaatatat tttttattac catagaagca atcagaaaga ttactcctat
     6781 ggctactttt ttatttggat ttactttact taatctattt gtatatgcat atttatcaat
     6841 tataagcata attaacttct ctttcctttt tgaacaccta atatgtaccc tattacacca
     6901 gcacctaacg ctgcttgtgc agagaataat aaactttcta tttctccact tggtggctca
     6961 tatgggctac taaaccatgg ttcataatct ggatttatct tagtaatttc actctcagct
     7021 tgaccatctg ctcctccata ttcagctccc gaattaacaa gtaatgggaa tatgataaga
     7081 gctactacta ataaaagtag taatatatta gttttcgtct ttgttttagc actcattagc
     7141 tttaactcct ttatcattat tttctagtat gcttttttct ccattttctg taattaaatt
     7201 atatacaatt acagtaagaa taccctctgc tattgctatt ggtatttgag tcataaagaa
     7261 aattcctaaa aacttaattg ctgaagccat cactccacct gatgggtctg ggaaagccag
     7321 tgctaactgt atcgaagtta ctgtatatgt agccaaatct cctattgctg ctgcaagaaa
     7381 tactggcatt gcattatttc cgtctttttt ctttaatgct ttaaatatta agaatgatat
     7441 aattggtcct ataatagcca ttgaaaaagc attagctcct aaagtagtta ttcctccatg
     7501 tgccaatagt aatgcttgga atattagaac tatagttcca agaacaaaca ttacacttgg
     7561 tccaaacatt attgctccaa gacctactcc agtaggatgt gaacaacttc ctgttactga
     7621 aggtatcttt agtgctgaca atacaaatac aaatgctcca cacagcgcta ataatacttt
     7681 ctttttaggg tcttgcttta ctatttttcc tattgatttt agtcctacta aaaaaaatgg
     7741 tataaatata actccccatg ctatagacca ttttactggt aagtatcctt ccattatgtg
     7801 cattgcattc gatgtaactg gagttaaaac gattaacata agtgcagcta taacccctaa
     7861 tttgatattt tgtttcattt tactatcccc tctctccctt tctaataatt aaaatataaa
     7921 taatatatgt ctgatttatt ttaaaaataa aaataaaaaa gcctaactcg atggctaggc
     7981 ttctaatttt cgataatcca aatacaaaca cataaaatga acatatctgt ataattatta
     8041 taagaaactt tctccatccc cgaagaaatt actttaatat tttaggcagg tctcctgact
     8101 tagcttcttc ctactcctct tccttcccat gagatataaa aaatatcatc atagtggcta
     8161 acaaaagttt cgtctgcatc acagtagcgg gggctgtaaa ggaatttaac ctttttccct
     8221 attaatcttg cttaaagaac ctataatatt atatatttaa tttttaaatt attcttaa
//
LOCUS       DAMJVX010000154         8178 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMJVX010000154 DAMJVX010000000
VERSION     DAMJVX010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8178
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.160"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(18..1658)
                     /gene="bclA2"
                     /locus_tag="PC407_002953"
     CDS             complement(18..1658)
                     /gene="bclA2"
                     /locus_tag="PC407_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HDF2498378.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGANGITGPTGATGATGA
                     NGITGPTGNKGATGANGITGPTGATGATGANGITGPTGNTGATGANGATGLTGATGAT
                     GANGITGPTGATGATGANGVTGATGPTGNTGATGPTGSIGATGANGVTGATGPIGATG
                     PTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGLVGPTGATGATGV
                     AGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGATGANGVAGPIGPT
                     GPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGPIGPTGPTGANGTTGATG
                     ATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLGNNTLVTFNSSFINGTNITFP
                     TSSTINLAVGGIYNVSFGIRAILSLAGFMSITTNFNGVAQNNFIAKAVNTLTSSDVSV
                     SLSFLVDARAAAVTLSFTFGSGTTGTSPAGYVSVYRIQ"
     gene            complement(2036..2575)
                     /gene="hpt"
                     /locus_tag="PC407_002954"
     CDS             complement(2036..2575)
                     /gene="hpt"
                     /locus_tag="PC407_002954"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2498379.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(2903..4210)
                     /locus_tag="PC407_002955"
     CDS             complement(2903..4210)
                     /locus_tag="PC407_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDF2498380.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDEKLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4635..6410)
                     /locus_tag="PC407_002956"
     CDS             complement(4635..6410)
                     /locus_tag="PC407_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2498381.1"
                     /translation="MMVMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDVCRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6407..6544)
                     /locus_tag="PC407_002957"
     CDS             complement(6407..6544)
                     /locus_tag="PC407_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498382.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6622..7191)
                     /locus_tag="PC407_002958"
     CDS             complement(6622..7191)
                     /locus_tag="PC407_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2498383.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7375..8010)
                     /locus_tag="PC407_002959"
     CDS             complement(7375..8010)
                     /locus_tag="PC407_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2498384.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         2329 a         1643 c          951 g         3255 t
ORIGIN      
        1 tcttttcatc atataaacta ttgtattcta taaactgata catacccagc tggagaagta
       61 cctgtcgtgc ctgaaccaaa tgtaaagctt aaagtaacag ctgctgctct agcatcaact
      121 aaaaagctta aacttacact tacatctgat gaagtaagcg tatttactgc ttttgcaata
      181 aagttatttt gggctactcc attaaagtta gtagtaattg acataaatcc tgcaagtgaa
      241 agtatggcac gtataccgaa agatacattg tatatccctc caactgcaag atttatagta
      301 ctacttgttg gaaaagttat attagttcca tttataaatg atgaattaaa tgtcactaat
      361 gtattattcc ctaaacttga agaagatact gtaaattgtg cattgtttgc tgctaacact
      421 cctgttgctc cggtcgctcc tgttggacct gttgctccat ttgctcccgt tgctccggtc
      481 gcccctgttg ctccggtcgt tccatttgct cctgttggtc ctgttggacc tattggacct
      541 gctactccat ttgctcccgt tgctcctact gctcctgttg ggcctgttgc tccatttgcc
      601 cctgttgctc cggtcgctcc tgttgctcct gctactccat ttgctccggt tggacctgtt
      661 ggacctattg gacctgctac tccattcgct ccagttgctc ctactgctcc cgttgggcct
      721 gttgctccat ttgcccctgt tgctccggtt gctccggttg gacctactgc tccatctgct
      781 cccgttgggc ctgtcgctcc tactgctccg gttggaccta ttgccccagc tactcctgtt
      841 gctccggtcg ctcctgttgg acctaccaaa ccatttgctc cggttgctcc ggttgggcct
      901 gttggaccta ccaaaccatt tgctccggtt gctccggttg ggcctgttgg gcctgttgga
      961 cctaccaaac catctggacc tgttgctcct actgctccgg ttggacctgt tgctcctatt
     1021 ggacctgtgg cacctgttac tccatttgct cccgttgctc ctatacttcc ggttggacct
     1081 gttgctcctg tatttcctgt tgggcctgta gcacctgtta ctccatttgc tcccgttgct
     1141 cctgttgctc ctgttggacc tgttatccca ttcgctcccg ttgccccagt tgctccggtt
     1201 agtccggttg caccatttgc tcccgttgct cctgtatttc ctgttggacc tgttattcca
     1261 tttgctcccg ttgctcctgt tgctcctgtt ggacctgtta tcccattcgc tcccgttgct
     1321 cccttgtttc cggttggacc tgttattcca tttgctcccg ttgctcctgt tgctcctgtt
     1381 ggacctgtta ttccatttgc tccagtcgct cctgtatttc ctgttggtcc tgtagaacct
     1441 gttgttccat ttgctccagt cgctcccata tttcctgttg gtcctgttat tccattcgcc
     1501 ccggttgctc ccgtatttcc tgttggtcct gttattccat ttgctccggt cgctccggtt
     1561 gcgcctctag gccccgtcgg tcctgttgga ccagtagcac ctgttggccc tgttggacct
     1621 acatcttgat ataaacttgg acctgaaata tcactcatat aattaatcct ccttttttaa
     1681 agttagagta ttactctata aatattgagt attactctat ataattatat gaatcgacaa
     1741 tttcttatgt gataatgtat ctatttgctt atatcaacat atatgctttg attcttaaat
     1801 ttcattactt ttttgtaaaa tcttttacaa ttattagtac tcttttacaa ttattaatac
     1861 aattaaaaaa ctcatttccc atcaattatt taaaattttt ttattatgtt ataaaaaata
     1921 gataattaaa ttgcataaaa ttaatttaat actactttaa ttacttttta aataaaaaaa
     1981 cctcaacacg attaataaaa atcatattaa ggcatccaca aaattatata attgcttatc
     2041 tatcttcatc agtaacattg aatatataag gaacatttct ataataatca ccaaaatcaa
     2101 acccatatcc aaccacaaac ttatcttcta tttcaaatcc acaataatct ggcactaaat
     2161 caacttttct tctgcttggt ttatccaaaa gaacacagca ttttaaacta gcaggatttt
     2221 tagcttttag atgttttaac acaaaatcca tagtaagtgc agaatcagta atatcgtcta
     2281 caaccaaaac atcatatcct gctatatcag tagtcacatc tgaaaccact ttaacctttc
     2341 cagtactttc ttcattgttt ccataactag atgtagtcat aaaattaact ctaagtggta
     2401 aatttatatt tctaactaag tctgcacaaa atataaagct accttttaat agagaaacta
     2461 ccataagatt tttatcttta taatcttttt ctataattgc tcctaattcc cttaatttat
     2521 ctttaatttt ttcttctgaa tacaatactt cccatttttt tgtttctata tccataaaaa
     2581 gacctccaat tgtaataatc cttcttaatt ttataagaga ttgctcactt tttcaatact
     2641 cttattcaat attagtcaat attattgagt ttttatgttc attcaacaat tattaacaat
     2701 gtatcttata ttttaagtag ttcatatatc cacactaaat tacatactat atattttaga
     2761 caaaaaaaga aaaaacttgc actaacaagt tttttctatt atacataaat ttatacataa
     2821 tcatatttta ttagagggta ctaattaaat ttatttatgc tatttttgtt atacctcata
     2881 atacaaaaca tattataaag tactagtcaa ctttattcat atcattcatt acatttaaga
     2941 ttatctcatc tgttatagac atacctttgt cacttactct acctaagttt tgtatacttt
     3001 gctcaacact tgaaccaact ataccattta gtggtggtac aaaacaatct tgcttagcta
     3061 ttatagcact ttgtatagct gcagatgctg cagaagataa ttttaatgca caaccagctt
     3121 tcgcaccgtc acatatcatt ccacttaagt cagctatcat attttctata gctccttcta
     3181 tttgcttttc agtaccattc ataagccaag agataccagc agttgctcca gtagaagcag
     3241 caacaccaca tccacacaca gcagataatc ttcctgtata gttcttaata taaccagtta
     3301 ctagatgaga tatagctaaa gcctttgcta gtttttcatc agattgtggg aattttttat
     3361 tataagcaac gattggaaga attgctgtta atccatgatt tccacttcca tttgaactca
     3421 taacaggcat ttttacgcct gccattcttg catctgaagc tccagcagtt aacatcattg
     3481 cataatttat aacatcatta cctaaaagac cttcttctat agacttttta attccatatc
     3541 cgacaccaat accagttttt tgttttaatc catattcagc catctcttca ttcattttta
     3601 ctccatctag taaaaattct atatctttaa aatcaatttc ttctacattt tttattaatt
     3661 cttgtatagt tactgtatcc attaaacttt cagctttttc tgccgcatca tttgaagctg
     3721 atacttttgg ttcattgtct aataatactt ctccatcttt ttctaagaac gtaaaattat
     3781 catgtttagt tcttattctc actttagcaa tatgattttt tccttttaac acaacttcta
     3841 taaatacttt ctctcttgta tcagcaggtg ttattgatag tggagtatta tccatataat
     3901 cctcagctat tttaacttct tcttttgtta gagtttctaa cacacttaat ccattttcag
     3961 aatggccacc tactatacct agagctgctg caatttttaa tcctaatctt tcagtacctg
     4021 gtatacctac gcacatgcca tttttgtaga tactaggact aactaacata cgattttcaa
     4081 ctatttcttc tcctaataat tcctttgcct ttgcacatgc tagagctaag gctactggtt
     4141 cagtacaccc tactgctggt ttaacttctg cctttaacat ttccactaat tcttttctta
     4201 tatctctcat tatatataaa cctcccaatt aagtttagat ttagtactca aataattaat
     4261 taaataattt cttatctata tttatagcaa aatacatgcc atatttttta tatcttttta
     4321 tatttttagt cttatattct ataaccattg gtatgactgc ttctatttat gtattttttt
     4381 tatttacata gagtatttat attctttaaa atggagaaac atatgacaat ttcgcaaaaa
     4441 gacaaaaaaa agtctcagaa tgagattcta tatcattttg agacggctca aatgtgttat
     4501 tttttaactt ataaaaacca ttttttaatt cataaaaaaa gattatgcaa aacacttata
     4561 gtataagttt aaagctttct ttaaagttat gaatttagaa aaatttaaat tttatatagc
     4621 tttttattat attactatga aattatattg tatttttcaa tttttctata taatgtagct
     4681 cttgatattc caagtgcttt agctgttaat tctttatctt ttttataatc tttgtactta
     4741 tttaaagctt ttattatctc aatcttctct agttcatcaa gtggaattat cctatcgctt
     4801 ttaacttcta actcttctac agaggtattc ttaatttttt gaggaattac attcattgtg
     4861 attattgaag aagaagacat attcacacta tattcaataa tattctgcaa ctctcttata
     4921 ttccctggcc acttatattc catcatagca tcaacaaccc tgctatctat gtcaataaca
     4981 tttttatcta atttttttga atactcttta accatatgct tgattataag tgggatatct
     5041 tcttttctct ctcttaaact tggtaatttt ataggtataa catttaatct ataatataaa
     5101 tcttctctaa aagtgccatt ttgaaccatt ttttctaaat ctttatttgt agcagcaata
     5161 attcttacat ctattgagac atttgattta cttccaattt tattaagttc cttttcttgt
     5221 aatactctca ataactttga ctgtaaatgc aaactcatat ctcctatttc atctaaaaat
     5281 atacttccct tatgagctac ttcaaacatt ccaagctttc cacctttttt agctccagta
     5341 aaagctccct cttcatatcc aaataattca ctctctaata aattgtctgg tatagcagca
     5401 cagtttacag ctataaaagg attatctact ctattgctat ggttgtgtat ggctcttgca
     5461 aatagctcct tacccgttcc actttcccca gtaataagta ctgttgatgt tgatttagaa
     5521 gctattaggg cttccttttt agaatttctt atagctacac tttctccaat tatgttttcc
     5581 aattttattc tataatcttt attcatctta ttgtagtttt ttatagcatc tcttttatct
     5641 ataaaatcaa taacatatcc ctttaactca ttttttaata ctattttatt gatgttgtat
     5701 attcccttcg aatcatatcc gtatctttta taaaaaaaac tacacgattt gtctttctta
     5761 aagttattta tagaaggttt ctttataaaa tctaattcga caaatatgtt tttgccagtt
     5821 atactatcct ttatattgaa aagctcttta aattttgaat tatatttatc aatttttcca
     5881 tccacatcaa tagacacaac agctttgtcc atattgttta atagtgtctc taattttttc
     5941 ttttctaatt caagctcata agttttaatt tgtgctttta atttattaga tattaagtct
     6001 gccattttgc ctaaaaagtt tataagacca cttttattat tttctataat ttgtgcttgt
     6061 tcttttgtaa aggctataag gcctataacg ccatgagaat cgccatcaca tataatagga
     6121 caacacacct ctgcaaactc tacacaagcc ccacttttat agcattccct acatacatca
     6181 ctactctttg ggtcatcaat tagtattgtg atctgttctg taagtgattt tttaaaagcc
     6241 gtatatttat ctactttctc acctattttg tctatatata ctcctgtacc tgcaatccta
     6301 aataactcct tatttactat agttacatct atattgagta ctgatactat agcttcagct
     6361 atattttgaa tatcagattt tatatattct aaacctatca taaccatcat tccttatttt
     6421 tctttaacat aagtatttgt cttatttctt taagaatcaa gtatatctta tttaaagtaa
     6481 attggattga catcaggatt acaaagagca taacaattat tattaactca taatttattt
     6541 ccatctaaac atctccttat aacaaaagac taggcttaac ctagtctttt atattatatc
     6601 catatttata ttttaactca tttatcttct ttttcccaca taaatatatt ttaatttatt
     6661 tgactcttta atcataccat caaagtttaa tcttgaaaat atattaaata cttcatgtgg
     6721 atagtgaatc tttgcaacag aaccacaaaa tttatgttta agataattta acttaacttt
     6781 taaattcttt aaatttaaat cctctaatat aacctctcca ttgcccttta acactctttg
     6841 tatttcagat gaagcttttt cacaatctgt aataacatta aattggtcaa ctattattat
     6901 cttgttgaac tgattatcat caaatggtag aaactctaac ttacctttaa ctacattgca
     6961 attttcatta tacactagag aataacagac atcttcataa ttactattct ccagtatagt
     7021 tacagaagtt ccaagacctc tcaatctctt gcctaggttt cctatatgac caacaagcaa
     7081 tattttatca cctacattat actcaattgt atccaaaaaa cattctaatt catcagatgt
     7141 atatttagct ttaactggct gtttatttat ctgacataat atgcttttca caatgcttca
     7201 cctcttcatt tcctcaatta taaattatgt acagattaca ttataataaa tggcgagcaa
     7261 ctattcaaga gatatttcat tagtattttt ttaaacaatc tttatacatt ttaatatgat
     7321 attataccaa atttattagg attttattca taaaaatcag tctatttttt atgtttattc
     7381 attgttattt tcatccaatt tttttatttt cgatatggat acttcatatg ccattttttt
     7441 gaccacatta ttttcatcta tttttttctc atattctcta ctttgaaccc taccccaaag
     7501 ctgtattcta tcaccaacct ttagattctt tgcaaattta gcatttctac cccaaactat
     7561 tgatggtata taatcagatt tattataagg tctgtttata gcaactaata aatcggttat
     7621 ttcccttcct aaaggtgtct tcctatacac aggttcctta catacatatc catctaaaaa
     7681 tatactatta gggtctttat tttcctcgtc tatgcttttt atttctctga caaacactgt
     7741 aagcactaat ctatttttat tatttaatgt tttgttatat gatcttaatt ggcctacgac
     7801 attgactaaa ttttgtttat caaggtcaat atcttggaga agtctttctg atacagtcat
     7861 tggaagtata tcaaaagaat cgcttagtct gtttattttt attttcatat tataaaattt
     7921 ttctccaaat atctcatggc taaattctag cttattgtct aactcccctc ttaagtttac
     7981 tacgttattc tcatctttaa cagctatcat attttatccc tccacaattg ttaattctta
     8041 tatttctggt atcagcttat ataataaata tatttataag ggatgtattt tattacaaat
     8101 ctttgattgc tgtttaaaag tttttattta gttataaatt tcatttaata tttcactttt
     8161 ataattataa aattatta
//
LOCUS       DAMJVX010000155         8154 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMJVX010000155 DAMJVX010000000
VERSION     DAMJVX010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8154
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.161"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..449
                     /locus_tag="PC407_002960"
     CDS             <1..449
                     /locus_tag="PC407_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2498385.1"
                     /translation="KQEPCDFSHGRFRFGGKIIRRNEKTKFDWLSRDKEQVEKYINDP
                     FCGVVCSCGFFYDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRD
                     RYIKLGVKDVTCKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            914..2128
                     /locus_tag="PC407_002961"
     CDS             914..2128
                     /locus_tag="PC407_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2498386.1"
                     /translation="MQIKIGKVIQRLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEKVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNIFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLWYANLSLEIKKMLSEDKGAFFSMYVDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(2548..3069)
                     /locus_tag="PC407_002962"
     CDS             complement(2548..3069)
                     /locus_tag="PC407_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2498387.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSYLQTEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            3425..4222
                     /locus_tag="PC407_002963"
     CDS             3425..4222
                     /locus_tag="PC407_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2498388.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            4332..4922
                     /locus_tag="PC407_002964"
     CDS             4332..4922
                     /locus_tag="PC407_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDF2498389.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNEETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFADFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            4988..6685
                     /locus_tag="PC407_002965"
     CDS             4988..6685
                     /locus_tag="PC407_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2498390.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGISLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     NVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            6899..7738
                     /locus_tag="PC407_002966"
     CDS             6899..7738
                     /locus_tag="PC407_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDF2498391.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT         3260 a          783 c         1352 g         2759 t
ORIGIN      
        1 tgaagcagga accctgtgac tttagtcatg ggaggttcag atttggtggc aaaataataa
       61 gaagaaatga aaagactaag tttgactggt taagtagaga taaagaacaa gttgaaaaat
      121 atataaatga cccattttgt ggagtagtgt gtagttgtgg ctttttttat gatttagttc
      181 aagggttaaa agaaattgag gataaagaaa acttaaaaaa agtgccactt gatataccta
      241 tttacataat atctggagat aaagacccca taggaaaaaa tggtaaagga gtcttaagac
      301 taagggatag atatataaaa ttaggagtaa aagatgtaac ttgcaaactt tacaaagatg
      361 gaaggcatga attgttgaat gaaataaata gagaagaagt ttttgaggat ataatttgtt
      421 ggttaaacaa taaaatagaa atattgtaat aaaaaaatat aatgatatat ttgctttaaa
      481 tttatttata tataatattt gaaaagtgga ttatgtatat gcaaagtaga aaagagagag
      541 cctagaagtg attttttgct ataatgccat ttattgaata ggtgggtgtt attataaata
      601 aattattaac actcagtatt attatcattg tatatggaat ttattttatt taaaattcct
      661 tgaaagttta aatatagtaa gttgtagtaa agagaaaatc taaatattta aatgctattt
      721 aatgtgaatc tattaattag gttatactta aacttaatta atagatttta tatttcaagc
      781 caatgagata gggttttaga ttaaaatata accaatggtt aaattacaga cattttgtta
      841 actttccctc gatttactag taaatatttt ttttatatac ttaaattaca acagatgtac
      901 tagggaggta tatatgcaaa ttaaaatagg taaagtcata cagcgtctta gaaaagaacg
      961 aaatttaact caagaacaat tagcaaagtt tataggtgtg tcaacaccag cagtatctaa
     1021 gtgggagagt ggaaattctt acccagatat agaattattg cctttattag cagacttttt
     1081 taatgtgtct attgataagc ttttaaatta caagattgat ttaagtgaag aaaaagtaat
     1141 gaaaatttat aaagaattag aatcaggctt tgctagaatt gaaattgatt tatcaacaga
     1201 agaaccaaaa gaagaattta gacaagactt agaatctgta aaaaagcttt ctaatatgta
     1261 tatagagaag tatcctaaaa gctatttatt gaagctaagg atatgttcgc tgtatcagat
     1321 gtattcatat aaatttggta agagtgaact taatgacaga gtaaaagaaa caacgaatat
     1381 tcttgaagat atagtgagaa atacagatga tatccaaata aaagaaacag ctcttattat
     1441 attatcaaac gcatattgta tgttggaaga ttatgaaaag gcagagttgt atttaaatat
     1501 gatacataag tcaatagggg atacaagtgt taatttagct atgatatatt taaagcaaaa
     1561 tagacttgaa gaagcagaaa tattgcttca aaataaattg tttagtaata tatttaatat
     1621 aagcatggat tgtaagggaa taattaatgt atacaaaaat caatacaagg aattaaagaa
     1681 aaaattggag aatagaaatt ttaataaaaa tgcaattgag agagaaatgg agtatataaa
     1741 aaataagttg ctgtggtatg caaatctttc tttagaaata aaaaaaatgc tcagtgaaga
     1801 taaaggtgca ttttttagta tgtatgtgga ttatatggaa ttatctctaa tttttttatt
     1861 ttttaacatg aaagaagaag caaaaaaagc cttatatagt ctaaaagaaa tattagaaaa
     1921 atatcctata catgaaagtt tggatgtaag tcaaatgaga ttttttgata aggtagagtc
     1981 taaaaattta tatactttta atatatatac aaacttgtta attgtactta atgatgacag
     2041 ttataatgag cttagagaag aaccaatttt taaggatgta attgaaaaga ttttagatat
     2101 ggaaaaaatg ttgaaaaata aagaataagt aaaaatttaa tataggactt aaagattaat
     2161 caaattaaaa gaaagtgttt caaaataaaa atttgaatca ccttttttgt tggtcttaaa
     2221 ccgttattta gaaaaataga ggtttcaaaa tagaaatctg aattatctgt attattacac
     2281 aaaaatagaa acctaaattt ataaaaatag aataaagttt gttatataaa atgtaatttt
     2341 gtctataaaa tctcacatgt tttcttaact ttaagcgtga aaaagaggat gtatcatgaa
     2401 ttttttagtt catgatgcat ccttatttgt atgaaatcca atatatcttt attatttaaa
     2461 caatggaaaa gagtcttatt tctattttga gacatctctt ttatttaatt atgaagtttt
     2521 atacagttca ataaatagga attatattta gaaactcatg acattatttc ttagatagtg
     2581 attaaattta tcatattcca ttttagataa cttttctggg taagacatta caatcaggtt
     2641 atgcccattg aatttttcat ggtatggtgg atgaatgtgt tcataattat ttgtcttata
     2701 ttttactctt tcatagaacg ccttgcgata aattgaaatc tcatcaattg gaaggtcaat
     2761 ttctaaaata atagtttttc ctttcttatt taaaagctct aacgctcttt gtccatatct
     2821 attattgcgc atttctggaa aaatgcagaa atgttctaca tagataaagt tatctgtttc
     2881 ccaacataaa attatgccga caaactggtt tttatcataa attaaattga attgatattc
     2941 tttatttttc aaaatttctg tttgcaaata agattttctt tgttcatgaa atgggaaact
     3001 aattttatat aattccatag ccttgtaata taatttattt tcactggttc ttaaccgttc
     3061 aaatttcact ttactcctcc taatcacgaa aattccaatt tatttatgag ttcattttac
     3121 catagtcagt atttgcatac aatgtgtttt aagttgagtg tcatttcttt cttttattta
     3181 tgcttatatc tcatagacta agagataatt gcttaagaca taactaaaaa ctattatatt
     3241 aagctttgat aatctaaaaa cagtatattt atggactttt ctaggtctaa aagtaatctt
     3301 aataatttta gaagttacaa aaaagttaca taaatttgga aagctataat atacatatat
     3361 aatataaggt taaaactgta atgaataata ttaatttaaa atttgaacat ggaaggagat
     3421 tattatggag aaaagagaaa agaggataat aatatcatct ttattaagtg tatcaatttt
     3481 aatgggtttg gtaagtatat attctatatt aaataaagaa gacataattt taactgttaa
     3541 gggtcaagag caaaaagtat cttcctttaa gaaaacagtt gaggaacttt tggatgaaca
     3601 gggtgtaaag tataattctg aagataagat taatccaagt ttagatacag aactaaaaga
     3661 tgatatgaaa ataaaagttg ttaaggtaac taaaagtaaa aaagaagaga ttgaaaaaat
     3721 tccatttgat acaaagcatg taaatgatag taatttgtta aaaggaaaat ctaaagttta
     3781 tcaagaaggt caagaaggag aaaaaaaact agtctataat ttaacttacc atgatggaaa
     3841 attagtcaaa aaagtcttat caaaagaagt aatatctaag gagccaacta caaaaattat
     3901 aaaatatgga actaaagaaa aagtactaat agcatcaaga ggagcaaata taagaggagg
     3961 caaacatatg aaagtggttg ccactgcata tgcaggagat acaataacat ctactggtac
     4021 aactccaaga tggggtgtta ttgcagttga cccacgtgta ataccatatg gaacaaaagt
     4081 atatatacct aaactgggta tgacttttgt agcagaagat tgtggaggtg caattaaagg
     4141 taatagaata gacattttta tgaacagtga aggaaaagct tcaaattggg gaaggaaaag
     4201 tatagatata tatctacatt aaattagtga atagaataac tttacttgaa ggacaaaaaa
     4261 gtataaaata tgaattaact aaactgttag attattctta aaacataaaa atatatctaa
     4321 ggggggataa gatgaaaata gtctgcttag gagacagttt aacgtatgga tttggagtat
     4381 cacgaagtaa ttcatggaca aacattgtaa atgaagagac tcgattagaa atagtaaata
     4441 agggtataaa tggtgataca acaagtggta tgttagctag gtttaatgaa gatgttgtaa
     4501 aaaactctcc tgatatagtt tttataatgg gtggcactaa tgattttatt gctggtgcgg
     4561 gaaatgaaat tattaattct aatataatgg ctatggtgca tcagtcatat tcaaaaaata
     4621 ttatacctat aataggtata ccattaaaac cagatattcc aaatgttagg gaagattgga
     4681 gttgttttac tgattttaat atagtatctc aaaacttaga atcatatagt tattggataa
     4741 aaaaattttg tatgacattt aatactaatt tcgcagattt ttattcagaa tataataaaa
     4801 atatggaact agaaggatat aaaaaattat attttgatgg gttacatcca accaaagagg
     4861 gtcatagaat aatggcagac atttttatta attcaattaa taagtatata gaagagttat
     4921 agattacatt aaaatgcttg tttatgcgtc agttgcaata aatacataca gtagagaggg
     4981 gtttattatg gattatacta aaacttatga agagtggata aagggttcat attttgatga
     5041 agatacaaag ctagagttgg agaatataaa aaataatgaa aaagaaatag aggatagatt
     5101 ctataaggat ttagaatttg ggactgcggg tcttagagga ataattgaag caggaacgaa
     5161 tagaataaat aagtacacag ttagaagagc tacttttgga ttggcaaact atatattaga
     5221 aaacacaaca aaagaagaaa caagtagagg agtagtaata gctcatgata atagacacaa
     5281 gtctagacaa ttctgtatag aatctgcaaa tactttggca gcatgtggta taaaagcata
     5341 tatatttgat agtttgagaa ctactccaga attatctttt gctgttagaa gcttaaatgc
     5401 aatcgctggt atagtcataa cagcaagcca taatcctcca gaatataatg gatataaggt
     5461 atactgggaa gatggagcac aagttatgcc agagattgca aatgcaataa cagagaaagt
     5521 aaatagtata catgattata gtacaatacc aaccttaacg gaagctaata aaaatttggt
     5581 agttttgttg gacgaatcac aagatactaa atttatagaa gcagtaaaaa gccaaattat
     5641 aagaaaagat ttagttaaaa atgtaggtaa aagctttaag atagtttata cacctctttg
     5701 tggaacaggg aatgttccaa taagaagagc attgaaagaa gttggttttg aaaatataat
     5761 tgtagttcca gaagaagaaa atccagaccc aaattttgca gggcttgatt atccaaatcc
     5821 agaagagaaa aaagctttaa atagaggtat attactcgca aaagaaaaag gagcagacct
     5881 tgtaatagca actgacccag attgtgatag agtaggtgta gcagtaaaaa ctactacagg
     5941 tgagtatgct ttacttacag gaaatcaaat tggtggtatg ctgacacatt atataataga
     6001 aggattaaaa gaaaataaca aattaaaaga aaatcctaca ataataaaaa ccatagttac
     6061 atcagaattt ggagcagata tagctaaagc aaataatgtt gatgtattaa atgttcttac
     6121 aggttttaaa tttattggtg aaaaaataaa attatttgaa caaaataaaa atagaagtta
     6181 tgtatttgga tatgaagaaa gttatggtta cttagttggg actcatgcta gagataaaga
     6241 tggtgtagtt tcttcattgt taatatctga aatggcagca ttttactatt caaaaggtat
     6301 aagtttatat gatggtttaa tagaacttta taaaaagtat ggatttttca aagaacagac
     6361 tatatctttg actttaaaag gtatagaagg tgtagaaaag ataaaagaaa taatatctta
     6421 ttttagagaa aatcaaattg attatataaa tagtattaaa gtagttgata aaaaagatta
     6481 taaaaatggt atagataatc ttcctaaatc taatgtttta aaatactttt tggaagatga
     6541 atcttgggta gctataagac catctggaac agaaccaaaa cttaaatttt acatagctgt
     6601 aaaaggagct agtgatattg aagcagataa aaagattcaa ggtttgaaaa agtatataga
     6661 tgacatggtc gaaaaattaa aataaaatat tattatttta taagataatt ttgtaataat
     6721 atttatttag ctgaaaatta taatttactt tcattgagta gaatatggaa atgttagtag
     6781 aattgttatt ttcagctttt atttgtgtta tttttactta aataattata ttatagaatg
     6841 aatctgagta taattaatat tgtataatga aattaatttg atagaaaagg ggatatttat
     6901 gtttggtata gataagaaat tgattgaata tataattgca aaatccaatt acagtgaatt
     6961 aagttcaaag tcagcagaag tatctttctt cttaatgttg tccatatttc cttttttaat
     7021 atttacaata agttctatag cttatatacc gattcttcat ttaaataagt atatagcttt
     7081 gtttagaaat atgatgccag aaggtgcttt tgctgtgtta tcttcaatca tagtttctgc
     7141 aatagacaat agaaacctga aatttttagc agtcagtttt gtacttacta tgtggacatt
     7201 ttcaagagca gtaaaagctc ttattaaagg aatgaataga gcctataaag ttaaagaaac
     7261 taggtctttt tttaaaattt tatctatatc atttttattt acaataatgc ttttggtcct
     7321 tattttttta tctatgatat ttttagttta cggggaaaaa ataggttatt ttatttttaa
     7381 tcttgtagga ttggatgaga tatttataaa aatatgggat attctaagat acactgtagg
     7441 tataataaca ataatagtga tatttacact tttatataaa tacactccaa ataaaaagtt
     7501 aactattaaa gaatctgcgc caggtgctat ttttgctact tttgcttggt ttttagtatc
     7561 atttttatat tcttattata caaattacta tgctaactat gaagtaatct atggaagtat
     7621 agctgaaata attgtactta tgacctggat gtattttagt agttggtcta tcgttattgg
     7681 ctatgaggta aattcaagat tgtattttag aaagataaga catgaaatgt taaagtaaag
     7741 tatttaaaat aaaagagagt atttagtatt ataaatgtaa ttgaattaat aaagaaatat
     7801 tgacatttaa gttaaaaagt ctagaatttt tctgggcttt ttttattttt agtataactt
     7861 gacaaatttt atttaatggg atagttttaa tttataatca tctttgaaaa gatgactatt
     7921 gataagtaag gtataaaatt ggtaagtatc aaaaaatata ctaaataaaa attatagtcc
     7981 taatgagtta atttctgtat tttcaaataa tgcagtattt agtcatgtgt ttttatatgc
     8041 gcctgtattt ccaattacca atatattaga ataagctact gtaattagat ttagaaaaaa
     8101 gcgacataaa taattataaa caaataataa attacataaa attaaattta ttca
//
LOCUS       DAMJVX010000156         8102 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMJVX010000156 DAMJVX010000000
VERSION     DAMJVX010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8102
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.162"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..597
                     /locus_tag="PC407_002967"
     CDS             <1..597
                     /locus_tag="PC407_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2498392.1"
                     /translation="GLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQS
                     LSLIMSCLTSPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALH
                     YGDRLIMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            728..1750
                     /locus_tag="PC407_002968"
     CDS             728..1750
                     /locus_tag="PC407_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2498393.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRIGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(1914..2471)
                     /locus_tag="PC407_002969"
     CDS             complement(1914..2471)
                     /locus_tag="PC407_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2498394.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2477..3553)
                     /locus_tag="PC407_002970"
     CDS             complement(2477..3553)
                     /locus_tag="PC407_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDF2498395.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3554..4237)
                     /gene="modB"
                     /locus_tag="PC407_002971"
     CDS             complement(3554..4237)
                     /gene="modB"
                     /locus_tag="PC407_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDF2498396.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4386..5198)
                     /gene="modA"
                     /locus_tag="PC407_002972"
     CDS             complement(4386..5198)
                     /gene="modA"
                     /locus_tag="PC407_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2498397.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKTDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEVESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5391..6320
                     /locus_tag="PC407_002973"
     CDS             5391..6320
                     /locus_tag="PC407_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2498398.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6481..6930)
                     /locus_tag="PC407_002974"
     CDS             complement(6481..6930)
                     /locus_tag="PC407_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498399.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARVLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7133..>8102)
                     /locus_tag="PC407_002975"
     CDS             complement(7133..>8102)
                     /locus_tag="PC407_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2498400.1"
                     /translation="FKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGLEQLI
                     KNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMILVHG
                     IGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKFVKNK
                     FPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEKRIPD
                     ILINYVVICGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNSKKMY
                     DLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         2807 a         1332 c          981 g         2982 t
ORIGIN      
        1 gggcttataa aagaatcttc tggtcaagta ttattaaata aacataactt gtctaattta
       61 gctgaacaca gaagaactca aatagtaagt agagtttttc aagacccaag tcttggaact
      121 tgcccttcta tgactgttag agaaaacttg tctttagctc ttaacaaagg aaaactatta
      181 aatctaagaa aatgtcttcg ttacaaaaga gatttcttag aaaatttatt ggaaggtgta
      241 tctcttgatt taaaaaaata ccttgatgtt caagtaaaat ttttatcagg aggtcaaaga
      301 caatcattat cactaattat gtcgtgctta acaagtccat cagttttatt acttgatgaa
      361 catacagcag cacttgaccc aaaaacatca aatgaagtaa ttgaattgac taataagatt
      421 gtaagggaaa aaaatataac tacacttatg gttactcaca acttaaaaca cgctcttcat
      481 tatggtgata gattgataat gcttcataag ggtgaagttg tattagatgt aaaaggtaaa
      541 gaaaaagaac aattaacagt tgaagaaatc ttagaaaaat ttgaatatgc agtttaaaat
      601 aatatatcta ttgtattaaa ctcggcttta aaataaagtt aaaattacaa taattatggt
      661 tactttgtaa taattcatat gaatatatga acatatataa taataaattt ttgaggggga
      721 tataaaaatg attaataaaa aaagattggc aagtttgatt ttagctggtg ctcttagtat
      781 atctatgctt acagggtgtt cacaaggagg agactctggt aattcaaaac aagaaagtaa
      841 ttctaaaggt aaagaagtta aaaaaattgg tataactcaa ctggttgaac atccagcatt
      901 agatgccact aggataggtt ttgtaaaagc attagaaaag aacggattta aagatggaga
      961 aaatattgat atagacttcc aaaatgctca aaatgacatg cctactacac aaagtattgc
     1021 gagtaaattt gcatctgata agaaggactt aatatttgct atatctactc catcagctca
     1081 ggcagcattt aatgctacta aagatatacc tatacttatt actgctgtga gtgaccctgt
     1141 tgctgctgga ttagttaaaa ctcttgaaaa gccaggcaca aatgtatctg gaacatcaga
     1201 ttttgtttct gtagacaaag gtttagaact tcttaaaata tttgctccta aggcaaaaac
     1261 tataggagtt atgtacaata ctagtgaagt aaattccaaa gttcaagttg atgctttaaa
     1321 agaatatgca tctaaaaatg gatttaaagt tgttgaaaaa ggtataacta cttcaaatga
     1381 agttaaccaa ggtatttcta gtttagttgg caaaatagac gtattatatg ttcctacaga
     1441 taacttagtg gcttcttcta tgcctatagt ctctaaaata gctactgaga ataaaatacc
     1501 tgttatagca gctgaatctg ggccagttga aaaaggtgct cttgcttgtc aaggtatcaa
     1561 ctacgaaaaa ctaggttaca aaacaggtga aatggcagtt aaaattttaa atggcgaatc
     1621 agtatctgat atgcctgtgg ctacatcaga tgatacagat atcattgtta atgaagatat
     1681 attaaaagct ttaggtatgg aaaaaccttc taatgaaaat atttcttatg taaagactaa
     1741 acaagaatag aaagatattt tctatatacg attaatatgc ctgtttctat aaattaattt
     1801 atagaaacag gcatatattt ttattttaat gtattatcta tttaaagatt atatgtaaat
     1861 gctcttaata tgattgtata aattttactt gtataaactt tatacaatca tatttattct
     1921 tctattttct ttattactct acatggattt ccaacagcta cagtatttgg tggtatatct
     1981 tttgtgacta cacttcctgc acctattact acattatcac ctatagtaat tcctggtgtt
     2041 atgattactc ccccacctat ccatacatta tcacctattt ttacaggaga accatactct
     2101 atgcctgaat ttctaagttg tgcatcaatt ggatgatagg ctgtatagat ttgaacgttt
     2161 ggagctaaca ttacattgtc acctatttca attttacata catctaaaaa aatacaatca
     2221 taatttgcaa agaaattttc gcctacatgt atgttatatc cataatcaca cctaatgttt
     2281 tgttctacat ttatttgctt gccaactgaa ccaaatagtt gtcttaaaat atcttctctt
     2341 ttttcatatt catcctctaa agtattatta aacaatctag ttaatttttt acaatactct
     2401 ctttctttta ctaacaattc atcatttgca tagtatcctt ttccagacaa cattttttct
     2461 ttttcagtca tatagtttac tcctttttgt tataattgta tctaaagcaa aataaatatt
     2521 cctgtggaaa atctaaatat attctgtcac ctttcttaaa tctcatttta cttttttcca
     2581 attctatttg aataggtaca cattcgttag ataaatcagt ttttctaaca tatatagtat
     2641 atgtaaaagg attttctaca atgttaatca attcgaaaca taacttttca tttaaatctt
     2701 cattagaatc caagacctta atatgatgct ccctgattcc cacatattct atattatcac
     2761 ctatttctct atttaatgtc aactcgcatc cccagtctgt agcataaata gtatttttat
     2821 ccagtctatt taatttggat atatttttac accctgttat cttagcttct ataaggtact
     2881 ttggagattc aaagagctct tttttaggtc tctttggaag accttctcct ttgttgtaca
     2941 ctataatatc atcacaaact cggtaagctt cttcaatatc atgagtaaca aatagtatat
     3001 ctcccttgta atcttttaac atattcataa gttctttttc catattactt cttaaatgat
     3061 ggtccagtgc tgagaaaggt tcatctaaaa gtaatatatc tggagatgtt gccaatgctc
     3121 tagctagtgc taccctttgt tgttgaccac cagataattg ccaaggatat ctcccttcaa
     3181 acccatttaa tttaagtata tctaagtacc ttgctactat ttcctttttt tcactcttac
     3241 ttagttttaa aagcccaagt tctatatttt gacttacagt catatgtgga aacaaagcat
     3301 agttttgaaa taaaaatcct acctttctat cttgtgttga taaatttatc ttcttttcag
     3361 aatcaaatag aactctatca tttaatacaa ttttacctct tgttggtttc tcaagccctg
     3421 ctatacattt taatgtcata ctttttccag aaccagattc ccccaaaaac cccagagtac
     3481 ctttttcttg tttaatttct acctttagtt taaaagagga taaatctttt tcaatatcca
     3541 cgtataagct caactatcca cacctctttc ctataatttt ttgctgaaat tccgaccaat
     3601 aatttagtaa taaaatcatt gctatagata ttgctacaat aatcataacc cacatcatag
     3661 ctttattcat atctccgcct tcaacagcaa aaaatatagc tattggcata gtttgagttt
     3721 ttccaggtat atttccagct atcataagtg tagcaccaaa ttcgccaaga gctctggcaa
     3781 acgacagcac caatccacca attattccag gccatgctaa tggaactgca atcttaaaaa
     3841 atactttcca ttcattaaga cctaatgttc tagcagctga cagcatatta ttatcaatct
     3901 gttcaaaagc tgaccttgaa gttctataca tcataggaaa agacaccact actgctgaaa
     3961 ttacagttgc tgtccaacta aatattaaag tcctatcgaa atttaataaa aactttccta
     4021 taaatccatt cttcccacat agtaataata aaaagaaacc tacaactgtt ggtggcaaaa
     4081 ttagtggaag tgtaaatagt ccatctataa gacccttcca cttacctcta tagttagcca
     4141 taatatatga cacaaatatt cctattacaa aagttattat tgttgacaaa atagatgtct
     4201 taagtgatat ccataagggt gaccaatctg ttcccatctt tccctcctta attttaaatg
     4261 gaaatagtga aactaataac tctattaatt tcactatttc cattttatta taatacacaa
     4321 ttattttcaa ttttcattta tttatatata atttatactt aattacacat aatttattta
     4381 taagtttatt ctactttttt atatccaaat tcttcaaaaa tcttttgtcc tgactcactt
     4441 aataaaaatt cttcaaattt cttagcagca tctacgtttt tactagcttt aattactgaa
     4501 actggatatg taataggtga atgagttttt tcgtctgttt tttcaactac ttttatctta
     4561 tcattattga cagtatcact gaagtaaacg aatccaacat cagcatttcc tgattgcacc
     4621 catgctaaaa cttcttttac atcttttgca aatactagct tatcttttaa cttatctttt
     4681 aaatttaaat tagttaacac ctcatctgca tacttaccag ctggaacact ttcaacttca
     4741 ccaacagcaa ttttcttcac tttatctgta gtcaaatctt tcatacctga tacactactg
     4801 tcagcagaag aaataagaac taaatcattt tttactaaat ctttcatagt atcagaaact
     4861 aataacttct cttcatccaa agctttcatt tgtttttgac ctgctgaaat aaataaatca
     4921 caaggtgctc cttgttctat ttgttgttgt aatgagcctg atgctccata gttaactgta
     4981 agttttacat ttgaatcaac ttttttatat tcttcttcaa tctttgccat tgcttctttt
     5041 aaacttgcag cagccgatat atttaactct actgaatcac tacttttagt tgttttttct
     5101 tgttttccat catctgtttt gtcttcatta ctattgcacc caactgtccc caaagttaaa
     5161 catgcaacta atcctaatat tcccaatatt tttttcataa caatctccct ttttgtaaat
     5221 ttgatacaca ttttaatatg ttttattatg ttttatcatg ttttgttata tatagtttaa
     5281 ttatatatac atgataataa attttcaagt aatttattta cactactttt aaatattttg
     5341 ctacattttc ctttttttaa tgttaaaata accttatatg gaggtggatt atggactcat
     5401 tatcatcttt gacacctgtc gaggtagctg aattattaaa gataacaaaa aatactgtat
     5461 atgaattaat caaaagaggt gaacttcctt cttataaagt agggaaaaaa ataagagttg
     5521 atactaaaga tgttgaagaa tatatcaaca gccaaaaaac tggtaaagta aaggatacac
     5581 atttcaacaa taaaaatata gatataaata ataatttcaa aattattatt tctggtcaag
     5641 atgtaatatt agacatttta gctaggtctg ttgaaaaaaa attggatgga ataagtacat
     5701 ttcgctctta tataggtagt tataatgctt tgtatgagtt atataatggc agagtttcta
     5761 tagcatcatc ccatctatgg gatttcgaaa cggatgaata taatagcact tttgttaaaa
     5821 agctattgcc aggaatacca tgtgtactta taaatctagc ttatagaatg caaggtttct
     5881 atgttgctaa aggaaatcct aaagagataa caacctggga ggatttaaca aaaccaagta
     5941 taaccattgt aaatagggaa aaaggttctg gaactagggt tttactagat ggtaaactta
     6001 gacttctaaa ctttaatggt aaacatataa aaggttatga taatgaagaa ttatcacatc
     6061 ttggagttgc aagtactgtt tctcgtggaa tagctgatgt tggtttggga aatgaaaaag
     6121 ctgctttaca agtaaataat atcgacttta ttcctcttca caaagagcgc tatgatttag
     6181 ttattaaaaa agaagactta caaaatccag tttaccaaac tataattaac ataataaact
     6241 ctcctgagtt taaagctgaa cttcaaggtc ttggcggata tgatttaact gatactggaa
     6301 aaactatagc aaaagtttaa gattaaaatc gcaatctatt ttataagatt tataattttt
     6361 cacaaaattt ataacttaaa cacgaaataa attgcgattt tagtgtctca aaaaatttat
     6421 gtctttcatt ttatcatcca atacaaaata taatataatt aattgtgcaa tcatattata
     6481 ttaatatata tttacataat agtatttttc tttaatgtag attaacatat gatatgtttt
     6541 aaacatcaac acccttgctt tcttatatat atgtggcaac acatatgagt ttaataaatt
     6601 atatacaatg ttgctttctt cttgtatttt ttcaaccaac aagttattta taaaattacc
     6661 tttctcattt tcaataatct cactccaatt tatcttaact tttaatatat atcttcccac
     6721 taggtataca cactctacaa taaataataa aaatacaata cagtcagtac ctgttgtctt
     6781 atataaatta cccgatacta atggctttgc gaagaaatta taaaaattca aagtatcacc
     6841 cgtataatca aaaagtatta atagcactcc tagaactctc aatactttaa tattcaacat
     6901 gtttttatct ttattatttt tgtttttcat atatactacc tctcttttct ttagatttga
     6961 ttgtaatcta atactaactc ttttatttga ggtataaatg aatttaattg gtagttattt
     7021 tcatttttac tagaaattaa ttcacattac taaacaaaac ttatatttaa acataaaaat
     7081 agcctatcta ttttttaata aatacaaaaa taaataagct actaatattc aattaacttt
     7141 tatcacttaa aacattacta tctataaatt taaatatttt attgaagtat actgtactat
     7201 catgttcaaa tccatcaaca tgaccaatac cttctataag ccataggtct tttttatcat
     7261 gtttaactaa atcatacatt ttcttgctat tttgataatt acaaacagta tcttttgttc
     7321 catgtataaa cagtatgggt acattagatt tctcaactga ctcatatggt tttacatcac
     7381 tgtaccaaaa tccagattta aacttattat ataaatctcc acaaatcacc acataattga
     7441 ttaatatatc tggtattcgt ttttctagta ctcccattct aattgcatct ttcatttcag
     7501 aataaggaga atccagtata taaaaatcga ccttatcatc ttttgaattt atctctgaat
     7561 gcatagcagc tgtccctgct cccatggaaa atccatgcac tcctaatctt ccttcaggaa
     7621 atttattttt tacaaattta actagactat ctaaatcata cctttcataa agaccaaatg
     7681 tgtaattatc tccaccacta ttaccagtat ttctttgatt ataaactaat acattataac
     7741 ctttatctaa atatctatat gctaccttta acatttcata gtaactactt ccaattccat
     7801 gaacaagtat catagtatct cttgtcttta tatttgaagt tataaaaaga ctttcaagct
     7861 tgtaattatt agaactttgt atcattaatt cagatgtctt gtagtttttt aatttctcca
     7921 gtggtttatc ttttctctca gaaaatattt ttactatatc ttcgtttttt attaactgct
     7981 ctaaaccaac cgaactatta tacactaatt tgccaaatat ataagataca attaatagga
     8041 ttaatagaaa cacaattgat ataattataa atttatttct taaactaata caatttttaa
     8101 aa
//
LOCUS       DAMJVX010000157         7811 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMJVX010000157 DAMJVX010000000
VERSION     DAMJVX010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.163"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(187..942)
                     /locus_tag="PC407_002976"
     CDS             complement(187..942)
                     /locus_tag="PC407_002976"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2498401.1"
                     /translation="MLKVIINGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1383..2372
                     /locus_tag="PC407_002977"
     CDS             1383..2372
                     /locus_tag="PC407_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDF2498402.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMIVLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVINATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(2643..3359)
                     /gene="dapD"
                     /locus_tag="PC407_002978"
     CDS             complement(2643..3359)
                     /gene="dapD"
                     /locus_tag="PC407_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDF2498403.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLNEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(3583..4332)
                     /locus_tag="PC407_002979"
     CDS             complement(3583..4332)
                     /locus_tag="PC407_002979"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2498404.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDNEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(4399..5286)
                     /locus_tag="PC407_002980"
     CDS             complement(4399..5286)
                     /locus_tag="PC407_002980"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2498405.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(5403..6404)
                     /locus_tag="PC407_002981"
     CDS             complement(5403..6404)
                     /locus_tag="PC407_002981"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDF2498406.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(6829..7710)
                     /locus_tag="PC407_002982"
     CDS             complement(6829..7710)
                     /locus_tag="PC407_002982"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2498407.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
BASE COUNT         2660 a         1280 c         1015 g         2856 t
ORIGIN      
        1 gactataaaa taataaacaa tataattcca tataccaata aataagataa tctttaagaa
       61 aaatgattat atttaaaagg taagcataaa atttataaac ttttagaata gaaatctcaa
      121 tcaattagat attcaaattg atgaatatga atacctaaat agattgagat ttattatgta
      181 caaaacttat cctagtatat ctgacatttt ataaagtcca gattgcttac caattaaaaa
      241 tttagctgca actatagaac cgtttgcaaa tacttcatca gattgagaat gatggcttaa
      301 tgtaagaact tcattatcac ctgcaaatat gacatcatgg tcgctgatta tattgccacc
      361 tcttatagag tgtattccaa tttcatttgg ttttctttta caatctctac catatctacc
      421 atgtgtattt tcactttcag gcaagacttc tctaattgca ttagctatca tagtagcagt
      481 tccactaggg gcgtcttctt ttctattgtg atgtttttct atgatttcaa tatcaaaacc
      541 ttctaacatc tttgaagcat ccttaactaa ttttattaat atattcactc caagagatgt
      601 atttgatgat aataatatag gaatgttttt tgatgcttct tctattttat ttaagtcatt
      661 ttcatcatat ccagtagtag ctaatactac aggtgtatta ttttttagag catatgacaa
      721 tattggctct aaagtctcat gatgtgaaaa gtctattatg acatcagttt tttcagttac
      781 atcagtcatt gttttgtaaa gctttgtatc actatgatga cctttttcat cagcagacct
      841 agctacacca caaaccaact ctaattcatt atcttttttt acacattctg ctaaaactct
      901 tcccattgtt ccattatagc cattaattat aactttcaat ataaattcct cctatgaaat
      961 taataaaaat ttgttatatt tctataagtt tattttatca ctaatattat ttatataaaa
     1021 taatattagt gataataagc ttacaattta tcttacatta aaaaaattta agtggatttt
     1081 tcaaaatata gataaacttc tttttcattg tataaatgat aaaaatatat ttgacttcat
     1141 atcaaaaaga gtgccccatg aactaaaaag ttaattttga gataccatat tcatatatca
     1201 tatgatttat ttggaaatca atattaatac attaaaataa ctaaatacat gtatgcataa
     1261 agatgtataa aaaatatata aacatatagt attaatttta aattcaacaa tttaattgtt
     1321 aaaagtgtta ttattaaatt agaaatatct taacttaata tactgagatg ggagtgatag
     1381 atatgttaaa agaagaaaaa acttatcaag agaaagacgc aaaagtattc aatggagtgg
     1441 cagctttgat aatgattgta ttatttttag tagtttcaat aggtatcatg gtttttggga
     1501 tagtaaaact tgatagtgga tttactggaa taggttcact tatgttaatt ttgggacttg
     1561 tatttatagt agtagatttt atcctatttt ttgggctaaa aatgattaat cctaaagaag
     1621 ctatagttct agttttgttt ggtaactatt atggaactat aaaaaaagaa gggtactatt
     1681 gggtgaatcc attttgttca gcaataaacc cagcagtatc aagaattaat gtatcgaaat
     1741 cttcatctga tgaaaaggta gatatatcaa ctaattcaag gggcaaaaaa gtatctttaa
     1801 aaacaatgac tcttaataat gaaaagcaga aagtcaatga tttattggga aatccaatta
     1861 taataggagt ggttgtaatt tggaaggtta taaatgcaac aaaggcagta tttaatgtgg
     1921 ataattataa tacattctta tctattcagt gtgattctac tattcgtaat gtttcaagac
     1981 tttatccata tgatgtatct gaagatggag atgaaaaatc tcttcgtggt agtagtcagg
     2041 aaatagcaga tagattaaaa gatgaattac aatcaagggt tgacattgca ggaattgaag
     2101 tgtgtgaagt gagaattact catctttctt atgcaccaga gattgcagca gcaatgttac
     2161 aacgtcaaca agcagaggct ataatagcag cacgtaaaaa gattgttgag ggggctgtaa
     2221 gtatggtaga gatggcattg actaatttaa gtgaaaataa tgttgtgact ttggacgaag
     2281 aacgtaaagc agctatggta agtaatctgt tagttgtgct ttgtggcaat aaagatgcac
     2341 aaccagtagt aaacagcagt tctatttatt aaattataaa aagtttaata gtttattaca
     2401 ttgatatatt gaaaaattga aggaaataaa aatacttgtc atcaagaaat aaaaatgata
     2461 taagaatgaa taagagaatt gtaaaaaaat aaaatgatta taaataaaag ataaaaatat
     2521 tataaaaaag agggtgcctc tgatgaatta aattcataca gagacacctt tttaattaat
     2581 tttttaaatt agtatattat tgtttaatta atctttctaa ttaataatat agtactattt
     2641 aattagtcta aatttctaag gtcttccata agcttagttt tgtcagccgt tttttcatct
     2701 ttcattttta taactttagc aggagaacca gcaacaacgg caccagcttc tacatcagta
     2761 gttacaacag caccagctgc aacaacagca cctttaccga ttctaacacc ttcaagaata
     2821 actgcattag ctcctattaa aacatcatct tcaactataa caggagtagc agaaggtggc
     2881 tctaaaacac cagcaacaac agcaccagca cctaagtgaa catttttacc aagagttcct
     2941 ctagctccaa taacagcatt catatctacc atagaacctt caccaataac agctcctata
     3001 tttataacag ctcccatcat tacaacagca tttttttcta tagtaaccat gtctcttatt
     3061 attgcacctg gctcaattct tgcatgttca tttaaagtat tttttagagg aatagcagaa
     3121 tttcttctat catattctac atgagtatct gttatatcat tcccatgttt atctaatact
     3181 tccattatat cctcatagtc acctacaaat atataagaac cattacttcc aaatacctta
     3241 aatttatcag ttgattggaa gtgtaaagag ttagtattta catatacctt aacaggtgtt
     3301 ttctttttaa cttcttttat ataccttgct atttcataag catcgtttaa atttaccatt
     3361 tgttaattct ccttgtattt cattatattt cttttattat atacttattt gtcaatttta
     3421 gtatatactt acttcttatt atttatattt tattttagtt agtttttatt gtatagacat
     3481 agtattaaat aaatttatta tatatgtttg atttgtattg cgtattatta tatctatatt
     3541 gcatatgttt aatttatatt gtgtattatt atatttatac tattatccca acatgctgtc
     3601 catattataa aatcctggtt tttgcttaac taagtatttg gcagcagcta tagaaccact
     3661 tgcaaagata ccacgagatt gagcttgatg gcttattgta agaacctcat tgtctccagc
     3721 aaatagagca tcatgttcgc caacaatatt tccacctctt atagcatgaa taccaacttc
     3781 atttggttgt ctttttgcac tacgaccata tctaccataa ttattttcaa cgtttggcaa
     3841 aacctctttt atagcgttag caatcataac agctgtgcca cttggggcat ctacttttct
     3901 gttgtgatgt ttttctatta tttctatatc aaatccttct agtaatttag ctgcatcttt
     3961 aaccaatttt actaatacat ttacaccaag agacatatta tatgattgga atacaggaat
     4021 aactttagcc gcttcacgta ttttgttcat ttcgttatca ttatatccag tagtagcaat
     4081 tactaaaggt gttttagtct ttaaaacgta agataagata ttatctaaat tagagtgatg
     4141 agaaaaatct ataactacat cagcttcctc aactatagta ctcatatctt cataaatttt
     4201 aaggtcgcct tcatagctat ccagacctct agctgcaaca caaaccaatt ctaattcatt
     4261 atcttctctt atagtatcgg ctaaaatctt acccattttt ccatcataac cagttgctat
     4321 aacttttaac ataatttcct cctatgatta agggctaact tttgaccaaa cttacttttt
     4381 accatgcatt aatctttact atgcattaac ttttagtcca aagttaacta attctttctt
     4441 taacacttct aaatttgctg ggtccatttc tgctagaggt aatcttaaat ctccaacatt
     4501 gaatcccaat aaattcattg cagtcttaac tggtatagga tttacttcta taaataaagc
     4561 tgcgattaga gcatccatac caagttgtaa ttttctagaa ccttcaatat cgccttcaaa
     4621 gaatttagct accaaatcgt gagtttcttg agggcaaaca ttagcaagta ctgatataac
     4681 tccttgacct cctaaagata ataaaggaag tatagtatca tcattaccag agtagatagc
     4741 aaagtcatct ggaactaaac tagctatttc tgcaacttgt cctaggtctc cactagcttc
     4801 ttttacagca actatgttat caattttagc aagttcaact ataaggctag gacttatatt
     4861 tacacaagtt cttccaggga cattgtaaag tataattgga agtttaacac tgttagctat
     4921 tgtttcaaaa tgacgcttaa gtccagcttt attagcttta ttgtaataag gtgttattat
     4981 taatagagca tctactccta atttttcagc ttcttgactt aagttaactg agtgcatagt
     5041 atcattagaa ccacttccag ctataacagg aactctttta ttaacttttt caacaacata
     5101 cttaatagta gcaagttgtt cttcatcagt catagtatta gcttcaccag tagtaccaca
     5161 agatactata gcatcagtac cattagctat ttggtattca actaattcac ctaatttgtc
     5221 aaaatcaact ttgttatcct ttgtaaatgg tgtaactaga gcaacagcag aacctttaaa
     5281 tatcatataa aatcaatccc ctctttttta tttttgtgtt ttatattaca gttacttcta
     5341 atgcttgttt ttgagttact attaatattt tacaaaatta tatatttata caagtttgta
     5401 tattatacta aatcgtatga taaagccatt tcagctattt gaattgcgtt agttgcagca
     5461 ccttttctta tattgtcagc aacaacccaa agatttacac cattatcaac actaaaatct
     5521 cttcttattc ttccaacaaa tacttcatct ctatcatcaa attctacagc agttggatat
     5581 tcgtttttag caggattatc aactactaca acacctggag cttcttcaag tgtttttact
     5641 aaatcatcta actcaaatgg attgttaaac tcaacattta tagattcact atgaccattt
     5701 tttactggaa ctcttacagt agttgcagtt atttttaaat catagttatc aagtattttc
     5761 atagtttcat ttaccatttt catttcttct ttagtgtatc cattttcagt gaatgaatct
     5821 atatgaggaa gacagttgta agagatttga tgagggtaga atccagtagg attaccgttc
     5881 attcctttct ctaaatcttc aacaccttta acaccagaac cagatacagc ttggtaagta
     5941 gagtaaacaa ctctcttaat tttgtattta tcatcaagag ctttaagggg aaccatagct
     6001 tgtatagtag agcagttagg attagctatt atacccttat gattttttat ggcttctgga
     6061 ttaacctcag gaactactaa aggcacatct ggattcattc tccattgact agagttatct
     6121 actactaaga ttccatttga agcagctatt ggagcatact tttcactaat acttccacca
     6181 gcagagaata atgctatttg tatgcctcta tcagtaaatg aagtttcctt taattcttct
     6241 actatatatt ctttaccaca aaacattact ttagaaccag ctgatttaga agaagagaat
     6301 aaaaatagat tttctattgg aaaatttctt tcttctaata cttttaaaaa tgttctacct
     6361 accattccag tagcaccaac tattgctaca tttacttttt tcatataaat tcaccccaat
     6421 tttaaattat taaaacatta ttttatttta gttatacaag taagatatgt taatattttt
     6481 atatatccaa aaactttaat attatattaa caactttaat tatatacaaa aaacgtacaa
     6541 aggtatactt tgtacgtcta atttatcact caaagtaaac cctgtagctc atcacctaca
     6601 aaaataagtg acagtattac acatgttatg tgcaatccca gaaaatatta ttgcaagaaa
     6661 tattttcttc ggctgtaaat cctttttcca atcattaaca cctgccagct actattggat
     6721 actcttagtt acagctacct ctaccctagg ttaataggat attaaaattt gattattcta
     6781 ataataatac atatttaaga aaaaatcaac ttatttatat aaatattgct aaaacttaaa
     6841 tccaaaattt gtcaattctt gttttaacac attaagatta gtttcttcca tttcagctag
     6901 aggaagtctt aaatcaccaa cattaaaacc taaaatgttc atggcagttt taacaggaac
     6961 tggatttact tctataaata aagcagcaat taaagcatcc atatcaagtt gtaatttttt
     7021 tgagccttca atatcacctt caaagaattt agttactaaa tcatgtgttt ctttaggaca
     7081 tatgttagca agtacagaga taacaccact tccacctaat gataatagtg gtagtatagt
     7141 atcatcatta ccggagtata tagcaaagtc tttaggtact aatttagcta tttctgcaac
     7201 ttgagctaag tctccacttg cttccttcac agcaactata ttatctattt tagcaagttc
     7261 ggccacaaca gatggcttta tattcacttt agttcttcct ggtacgttgt ataaaataat
     7321 tggcaactta acgctatttg ctattgtttc aaagtgaagt ttaagacctt tctcattagt
     7381 cttattatag taaggagtta ttattaaaag accatccacc cctaattttt cagcttcttg
     7441 actcatatga acagacaaca tagtattgtt tgaaccagta cctgctataa caggtattct
     7501 cttattaact ttttcaactg tatattttat tacagcaaaa atttcagatt cactcatagt
     7561 agtcgcctca cctgttgttc cacaaacaac tagtgcatct gtgccattct caatgtgata
     7621 ttcgattaat tcacctaatt tttcaaagtt gacattatta tcctctgtaa atggggtaac
     7681 taaagcaaca gcagaacctt taaataacat ataatcaaat ctcctttttt atctaaattt
     7741 aattttttaa gtacaataat aattatagta caaattaatt aattttaata ggtatagtta
     7801 atatcttaac t
//
LOCUS       DAMJVX010000158         7765 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMJVX010000158 DAMJVX010000000
VERSION     DAMJVX010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7765
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.164"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            106..438
                     /locus_tag="PC407_002983"
     CDS             106..438
                     /locus_tag="PC407_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDF2498408.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            600..785
                     /locus_tag="PC407_002984"
     CDS             600..785
                     /locus_tag="PC407_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498409.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            812..1897
                     /locus_tag="PC407_002985"
     CDS             812..1897
                     /locus_tag="PC407_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2498410.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLYNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSTIPKMKEVIGNHA"
     gene            1890..2348
                     /locus_tag="PC407_002986"
     CDS             1890..2348
                     /locus_tag="PC407_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498411.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            2383..3474
                     /locus_tag="PC407_002987"
     CDS             2383..3474
                     /locus_tag="PC407_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2498412.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKAGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            3479..3703
                     /locus_tag="PC407_002988"
     CDS             3479..3703
                     /locus_tag="PC407_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498413.1"
                     /translation="MENIKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            3713..4276
                     /locus_tag="PC407_002989"
     CDS             3713..4276
                     /locus_tag="PC407_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498414.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            4237..4866
                     /locus_tag="PC407_002990"
     CDS             4237..4866
                     /locus_tag="PC407_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2498415.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            4906..5043
                     /locus_tag="PC407_002991"
     CDS             4906..5043
                     /locus_tag="PC407_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2498416.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            5043..5795
                     /locus_tag="PC407_002992"
     CDS             5043..5795
                     /locus_tag="PC407_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498417.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            5808..6293
                     /locus_tag="PC407_002993"
     CDS             5808..6293
                     /locus_tag="PC407_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498418.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYENIMVVDGNSINFIYPVSALQTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            6300..6449
                     /locus_tag="PC407_002994"
     CDS             6300..6449
                     /locus_tag="PC407_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498419.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(6585..7406)
                     /gene="yidA"
                     /locus_tag="PC407_002995"
     CDS             complement(6585..7406)
                     /gene="yidA"
                     /locus_tag="PC407_002995"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDF2498420.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMTLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNEAPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
BASE COUNT         2991 a          856 c         1365 g         2553 t
ORIGIN      
        1 taataatata gctaatatct attttacaga taaataagaa taaacttata atataattgt
       61 atctgtaaaa ttttgtaaaa tataaaaagt atagggggaa taaaaatgaa agtagcttat
      121 atttttagta gtactaatac acataaaatt ctagacaaaa tgataatacc acaacttgaa
      181 caaggaactc atggagtaga cgttctaggg atgtttttct ttatggataa taccttattt
      241 ttatctaaag gtaatccagt aggcgagaga ctaagtaaat tacatgaaaa aactgatatg
      301 ataataatgg cttgtgacca atgtgctata gaaagaggta tagagaataa tttggttgat
      361 ggagctacaa ttggatgttt cccaaaccta tatgcttgtt taggcagtgc aggtgtggac
      421 caagttataa cgttataaat tagtatagga tacaatccat agaaaaattt attttctgtg
      481 gattatttta agtaaaagat aaattgttta aagtagggga tgaacaaatg tctaaaattt
      541 tagttgaatt tataaacact tgaccatctt gtgatgaaca tggagtgttt ataaaaaaaa
      601 tgtcagaaaa gtatgctgat aaattggaaa taaaattata tcaagcaggg aaagattttt
      661 cgtatataaa aaaatatggg attataacaa aaggaacttt aataataaat cagaaaaaga
      721 aatatgatag attaaataaa gatacaattg aaagggcaat agtagaagct ataaacaata
      781 attaaaagtt taaaaattag gagtatcaaa aatgaatata ttagaaattg ttttaaatgg
      841 agttaaagat actatagtag cttctatagt tacactgaat ggagcatcag gatggcttat
      901 gataagtttt atattggcag gtgtacttca caatgtttta actccaagaa ggtttcaaaa
      961 acaacttgga aataagaaat tttcttcttt aattaaaagt actatctcag gaatgttttt
     1021 accaatatgt agttgtggag taataccact tggaataagt atgtattatt caggagcata
     1081 tttaggacca gtacttgctt ttatgacatc aactccaata ataaatccaa tagctgtagt
     1141 tctttgtcta ggtcttttag gtcctaaagt tacaatcatt tatctaatag ctggtttttt
     1201 agtaccattt ttagtaggat ttttaggaaa ttgttttgga aaagaagaat tatcacttta
     1261 taatgattca gaggaagaag taatagaatt agaagaagaa aaaagaagtt ttatggaaaa
     1321 tatgattgat ggtcttaaat ggtcttttgg tgaattagcc cttactatta gtaaatatgt
     1381 agtaatgggt atggtttttg caggatttat aacaactatt tttccaaata gtataattca
     1441 aaaatattta ggaaatccag gtttgctatc tcttggaagt atagcaatac ttgcatgtgt
     1501 tatgtatgta tgtgctgtag gacatatacc gtttatagca gcccttgttg caagtggagc
     1561 atctcctggt atagcgataa catttctaat agctggagca gctacaaatc taccagaact
     1621 tataagcatg tttaaactta ttggaaaaag aactgttatt atatattctg taactttgac
     1681 actgtcctca ataataattg gatatataac aaatttactt ttagtgaata ataattcagt
     1741 aaatttagat gcaacaaatt cttcaataca gttggcaaat aaactaatgt taaatatacc
     1801 tgatagtttt gagtatgtat gtactatttt aattatttta tttgcattta aatcaacaat
     1861 accaaagatg aaagaggtca taggaaatca tgcttaaaaa gatagttata atatcctgta
     1921 tagtagttgt gttaatcatt ttatcaaaaa tagttgatga taacatcaaa gaagatgctt
     1981 ctattccaaa tgtgaataaa gaaacattag aatattttag aaaaaattat aaagaagaca
     2041 taataacttg tgctgaagaa gatttaaata atgatgggaa aaaagattta gtagttatat
     2101 ataaaaaaag caataattca aatgaaatgg tagtcgtagt ttctgataaa aatagccact
     2161 atataacaaa gccaatacca gcacctatag agaatcaaac tataaccttt aaaaatattg
     2221 atgataaagc accaatagaa gtaatagtat cagggtctaa aaatggtaat gtaggatatg
     2281 ctatttacag agtagagggt aaaaaatttg ttgatttatt tggcgaagat atggacaaat
     2341 gttgctaaaa ataaaaataa tacataaagg tgggaaattt atatgaaatt atctaagaag
     2401 attcaagcac taatcttatt tggtgtaatg ggtacatcta tattaacagg atgtagtagt
     2461 aaaccaaaag aaaaagaaga agcaaaagct ggaaaagatg attatacaat aacagtaggt
     2521 tattataatt gtgaccatat gactgcggga ccagtagcag aatcggcagg aatatataaa
     2581 gatttaggtc ttaatgtaaa aactgttgga aatggtaaag ttcctgaagc tatggcagca
     2641 ggaaaaatgg atgcaggtta cataggaact aaggggttag ttggagcaat acctaagggt
     2701 tcaccaataa caatagcagc taacaaccat actggtggtt ctgaatattt aatagtttca
     2761 aaagatataa aagaacctaa agatttaata ggtaaaaaaa ttgcaacaga tatgagtgat
     2821 ttcttatgga caagtgatta tggtccagaa acaggtctac caactgaccc atcaaaatat
     2881 gaagttgtaa atatggattc agacaaagat aaatatttag ctcttaaaac tgggaaaatt
     2941 caagctttta catcttgtga cccttggggt tctgtagctg aaaatgacgg tgcaggaaaa
     3001 ataatagcat ctactcaata taaagaaaaa gctaatggca aagaatataa ctgttgttca
     3061 ttctcattaa acaaaaactt tataaaagaa catccagatt tagcaaaaaa attggttcta
     3121 gcacatacaa aagcaataga atatatatat acaaatccag ctgaagcagc taaaatattt
     3181 gcaaaatatt ataatgtaga agaagaagtt gcacttagaa ctatctataa gaagactgtt
     3241 ggagaaggta gaactcttac ttggaaagtt acaggtgaag aatataaaaa taatttacaa
     3301 atgtataaag atttaaaggc tttagatgat atacctaaat atgaagatac gattgataca
     3361 agcttattgg actcttgtgg agctgatgat tttgacaaat ttataaaaga aaaagttaac
     3421 ccagagtttc ctgaaggtat gagttatgaa gactggaaag caaaggtatt ataaataaat
     3481 ggagaatata aaaaatataa taatactttt tatcattatt ttgggaagta tatttataat
     3541 attttttaat tttgaagata aagaagaaat aaaaggaaaa ttaagaatag gggtatctga
     3601 tgatacctct ggttttgtaa taaattatat ggtaaataga gactactttg aaaatataaa
     3661 agtaagtgat ataatggata tttttacaat taatgattgc tgagcaagta caatgcagtg
     3721 ggctctgagt tcagaggaaa tagatatagc aatcatatgt aaagatgctg caaaacaata
     3781 tgtaaatgta aatagtggat ttgaaattgt tggtactgta gttcaaaatt ctgatatatt
     3841 tctattaagt gataataatc ctgaaaagat aggagtcatt caaaatagag attaccagtc
     3901 agaattggtt aaaaaatact acaagaatgt ggaagtagct ccattattag ggactgcact
     3961 agcatatgga ttagaaagta atttagtaga tggtgttgta atagattgca taaagtcaat
     4021 tgggttagag ggtaaaagaa aatctacaac taaattagga gattatgata cctatgtatt
     4081 agttgtaagt aaagagttta aaaaaagtaa agcatatagt gattttataa cactttacaa
     4141 taaaagtgtt gatgctttaa agaataaagc tacatttaag aaagcattaa aagagtacag
     4201 gaatataaga aatatagata tggaggaaat aagcaattgg aaattaaaat ttctaaagat
     4261 aaaagcgata gattaactaa gaaaaaaact gatggtagta tggatatttt atataaaata
     4321 atactattag ttattatatt tttagtctgg cattttgcag caaaagatat agggagttct
     4381 ttgttacttc caatgccagt agatgtaata aaaggtttct ttttctgtgt gacagatgca
     4441 gagacagtca ctaatctttt tataacattg cagagagtac ttaaaggttt tatgtatgca
     4501 ttattgtttg ggcttccaat aggatttatt atgggatttt ctaaaacttt tgaaagggtt
     4561 ttatctccag tagtagattc tgtaaggcaa gttccaataa tggcatgggt tcctcttact
     4621 atagtatggt ttggtatagg agatggtcca acaatatttt taatagcttt ttcaggggtt
     4681 ttccctataa ttttaaatac tattcaagga gtaagagcaa tatcaaagga ttattataat
     4741 gcagctagaa gtatgggagc atcgccaatt gtgatattta caaatgtaat tgttcccgca
     4801 tctcttccag atatactaac tggttcaagg attgctataa gtacaggatg gatgtctgtt
     4861 atctgagccg agtttatagc tacgagtgcc ggtctcggtt actcaatggt acaagctcaa
     4921 acattaatgc atacagatgt attaattggg cttatgatat ttgcagcttt aataggattt
     4981 ataatagata gggtattaaa gtttataaat aaaatccttt gtagatggag gtttgcagat
     5041 taatgaagtt aagtgtaaga gatataaata agacatttgt aaataataga gtacatacaa
     5101 aggttttaga agatataagt atggatatag atgatggaca atttgtctgt cttttaggac
     5161 catcaggttg tggaaaaaca actttactta ctataatagg tgggtttcaa aaatcagaac
     5221 gtggagatgt ttttataaat gataaaagag ttaaaaaacc gggcatagat agagctttta
     5281 tatttcaaaa ttatgcttta tttccttgga aaactataag aggtaatgtt ctatttccta
     5341 tgaaacagca gaaaatacca aaagaaaaaa gagaagaaat gctagaagaa cttttggtta
     5401 tgtcagactt gaaaggaaaa gaaaaactgt ttccgcatca attatctgga ggaatgaaac
     5461 aaagagtggc catgataaga gctttagcat gtaatccaga ggtattacta atggatgaac
     5521 cattaggagc tgtagatttt caaatgagac aaaatttaca ggaagagcta gaacgaatat
     5581 ggataaagaa gaaaataact gcattaatgg taactcatga tgttgatgaa gctgtatata
     5641 tgagtgatag agttatagta atgtcgagag ataaaggaag aattattgat gatataaata
     5701 tagatattcc aagacctaga attagaggaa gccaaaaata tgaagagtac aaaaacaaat
     5761 taactgatac tttatcaaag tgttatgaag tttaaagagg agtagacatg agtcaaatag
     5821 aaaaatacaa tagtttggat aatacacaac agaaaataag aatctctatt atggatatga
     5881 taatagataa aggttctagt gttacacttc aagaagtgac tgaatatgta agtaaaaagt
     5941 tgaatataga aaaagaatat atagaaagga cactacaata ttttatttat gaaaatataa
     6001 tggttgttga tgggaatagt ataaatttta tatatcctgt atcagcattg caaacaaatc
     6061 ataaagtaac tttgagagat aatagaagtt tcagcgcaat gtgtgctata gatgctatag
     6121 gaacctcttg cacattcaac caagatataa aaataaactc tatttgtagt gctacaggta
     6181 aggaaataga agtaattata aaaaatgaaa agattgagta tgtgaataat ccaaacttaa
     6241 gagtgcttca tataaatctt gataagcatc taaattgggc tgctagttgc tgaaatataa
     6301 tgaatttctt ctggacgaag agtgattttg atgcttggac aaacgaagcg ggattatctg
     6361 atgatgaaga tatatattgt ttagatatta atgaggcaat agtagaatca tataaaatat
     6421 ttaaactcaa acaaaaggta ctatcataga tatacatata tgaaaaacta aaaatgacct
     6481 ttagataaaa atgataggta atatccatac tatataaatt agtatagagt agggatgtga
     6541 aaagtattta aatctaaagg tatattttta gtttttttag atgattagat agccttatta
     6601 agtatatatt tatttataac ctttgcaact ccattttcat tattagtatc agtaacataa
     6661 tctgcaattt cctttatact attacgtgca ttccccatag ccacaccaag acctgcatac
     6721 tttatcatat gttggtcatt ttcttcatcg ccaacagcaa tagcattttc tataggtata
     6781 tttagattcg tacataatgt tttaagagca gttcctttat tagtagtttt acctaaaaat
     6841 tctagataaa aaggagcaga ccttactata gtatatttat tgaataaatt tttaggtagt
     6901 tgctgtatga tttcttccaa tctttctggt tcatcaacta tcataatttt acagatttta
     6961 atagattcat caattttatc tttttgtata tatttgactt ctatattatt taatttcgct
     7021 tcatgaagtg tatattttcc aggtgcttca tttggtgtaa ttaaatttgt cattgtattt
     7081 atgtgtattt tagtcttata ttttttacaa acgttattat atataaaatc aaaatcatct
     7141 aaagtcattt ctacatcatg tatgattttc tcacttttaa cttcttgaat aattgctcca
     7201 ttaaagcata taacataatc accttcatct gttaaattta gttgatttag aagttctttt
     7261 acaccaggaa gaggtctgcc tgttgttata actacttttg ccccagccct ttttgcctcc
     7321 tgaatggact caaagacttc aggagtaatc cttttttgtg tatttaatat tgttccatca
     7381 atgtctagtg cgattaattt gtacatatca acctccaata aattatatac tcaaatttcc
     7441 tgtgaaaacg ttgacacagc tagtgttgaa caaagtttca gtgggaaata tggtttgttt
     7501 tgtatactat tatatcaaga gataagtaat ttagaaagtt taataatatt aatttttgat
     7561 aaaaatatta cttatgtgag atgagaatgt aaaaactttg taatcaataa ttccaaaatt
     7621 cttaattaca aataatggta tcgtttgcat tgttaatata aataaatgta tattatatag
     7681 ttagaagttt gaataaatag actgatggta atttgttata ttagttagtt ttgtgttata
     7741 aaatattgta attccattaa tcata
//
LOCUS       DAMJVX010000159         7744 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMJVX010000159 DAMJVX010000000
VERSION     DAMJVX010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.165"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            351..1544
                     /gene="nifV"
                     /locus_tag="PC407_002996"
     CDS             351..1544
                     /gene="nifV"
                     /locus_tag="PC407_002996"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HDF2498421.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVI
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1569..3494
                     /locus_tag="PC407_002997"
     CDS             1569..3494
                     /locus_tag="PC407_002997"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HDF2498422.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSNVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3516..4511
                     /locus_tag="PC407_002998"
     CDS             3516..4511
                     /locus_tag="PC407_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HDF2498423.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGAEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4838..5311
                     /locus_tag="PC407_002999"
     CDS             4838..5311
                     /locus_tag="PC407_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2498424.1"
                     /translation="MVGSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5345..5839
                     /locus_tag="PC407_003000"
     CDS             5345..5839
                     /locus_tag="PC407_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2498425.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5936..6457
                     /locus_tag="PC407_003001"
     CDS             5936..6457
                     /locus_tag="PC407_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2498426.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMYDDNIEADKYIREELKVPSKYSVECIISIGYSDEEKKAYND
                     SDLDYKKVHFNNF"
     gene            6646..6957
                     /locus_tag="PC407_003002"
     CDS             6646..6957
                     /locus_tag="PC407_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2498427.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6985..7671
                     /locus_tag="PC407_003003"
     CDS             6985..7671
                     /locus_tag="PC407_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2498428.1"
                     /translation="MKYSYKKCIIDSILLMFIIQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT         3106 a          764 c         1495 g         2379 t
ORIGIN      
        1 tattaagata ctttaaaatg gaatgtaact taacttaaat tttagtagga attttatagg
       61 tttattttta aaggtttttt gaaaataaat ctatttaaaa attaaattaa aatgcaagaa
      121 aaacataata actttcattt ttaagaagtt ttcacaaaaa agaaaaaata tagtgcaata
      181 aatgatacaa agtaatataa tagattacaa gatatgaaaa gctaaaaaaa taaattgcaa
      241 atgaaattaa aatgaataaa aaataaaaaa ataatcataa gttagaggta tcaatgaaaa
      301 ataagactta atgtttttaa aattaaaagt atactaaggg gggcattaat atgtgcgtta
      361 tatcaaaaga tagagcaaag gaaataaaaa tagtagatac tactttaaga gatggagagc
      421 aaacagctgg ggttgtattt gcaaatagag aaaaaataat gattgctgaa atgttaagtg
      481 acctaggtgt tgaccaaatt gaagttggta tacctactat gggtggagat gaaaaaaatg
      541 taataaagca catatgtagt agaaatttaa aatcagatat aatggcttgg aatagagcag
      601 taataaaaga tgttgaagaa tctatttctt gtggtgttga tgcagtagct atatcaatat
      661 cagtatctga cattcatata gaaaataaat tgagaacttc tagaggctgg gttttagaaa
      721 atatggctaa aacagttgaa tttgcaaaga aaaatggatt atatgtttca gtaaatggag
      781 aagatgcttc aagagcagat atagacttct taactgaatt tataaatgtt ggaaaacaag
      841 caggggcaga tagatttaga tactgtgata cagtaggagt tatgaatcct ttttctataa
      901 agaatgcaat agaaactctt tatgaaagaa ctaattttga tatagaaatg catacacata
      961 atgactttgg gatggcaact gcaaatgcac ttgcaggaat agcagcagga gcaaattatg
     1021 ttggtgttac agtaaatgga ttaggtgaaa gagcaggaaa tgcagcatta gaagaagttt
     1081 taatggcact taagtgtgtt tataaatgtg atttaaataa tatagatact agaaaattta
     1141 gaggaatatg tgagtatgta gcacaggcat caggaagaat acttcctaca tggaaaccag
     1201 tagttgggga taatatgttt atacatgaat ctggtataca tgcagatgga gctttaaaag
     1261 acccgcataa ctatgaacca tttgacccaa gtgaagtaaa tcttgaaaga aaaatagtaa
     1321 taggtaaaca ttcaggaaga gctgcagtta taaataagtt gagtgaatat gaaatgtata
     1381 tttctccaga aaacgctacc aagctattaa atgctataag agctacatct ataagattaa
     1441 aaagaagttt aatggataaa gaaattcttc aattatattg tgatatacta gcacatgaga
     1501 aaggcacaac agaagaagaa gcagttagag gttcatatat ttaagatata attgtttagg
     1561 aggaaaatat gggagataat atagtttata aaataattaa aaaacatata gtagatggag
     1621 aagctgttgc tggaagttca attggtataa aaatagacca aactttaaca caagattcta
     1681 ctggaacaat gacatatctt caactagaag ctatgggaat agataaagta aagactaaaa
     1741 gaagtgtggc atttgtagac cataatatgt tacaacaagg atttgaaaat gcagacgacc
     1801 ataagtatat acaaacagtg gcagataaat atggagttta tttctctaag ccaggaaatg
     1861 gtatttgtca tcaagtattt ttagaaagat tttcaacacc aggagataca cttttaggtt
     1921 ctgatagcca cacacctact gctggaggtg tgggcatgat ggctattgga gctggtggat
     1981 tagatgttgc tttagccatg gcaggaggag catactatat aaaagcacca aaagtctgta
     2041 aggtgaatct agttggaaaa ttaaataata tggtatcatc aaaagatata atattagaag
     2101 tgttaagaaa acaaactgta aaaggtggag taggtaaagt ttatgaatat ggtggagaag
     2161 gtgtcaaaag cctatctgtt ccacaaagag ctacaataac aaatatgggg gctgaacttg
     2221 gagcaactac ttcaatcttc ccaagtgatg aaaaaacact agaatttttt aagagtcaag
     2281 gaagagaaga cgcatggata gagttaaaac cagatgcaga tgcagtttat gatgaagaga
     2341 ttactataaa cttggatgaa ttaaaaccat tagctgcaaa acctcatagc ccagacaatg
     2401 tagatgaagt tgaaaatata ggcaagatta aaatagacca agtggcaata ggaagttgta
     2461 caaattcttc ttatgaagac ttaatgaaag tagcacaaat cctgaaagga aataaagtac
     2521 ataaggatgt gagtttggta atagcaccag gttcaagaca agttatggag atgattgcta
     2581 gaaatggggc tttggcagat ataataagtg ctggagctag aatactagaa aattcttgtg
     2641 gtccatgtat tggtatgggt caatctccag gtactgactc tgtgtcttta agaactttca
     2701 atagaaattt ctatggtaga agtgggactt tgtcagcaca agtatattta gtaagtccag
     2761 aagtggcagc agtaagtgca atcaagggag tgcttacaga cccaagagaa tttgatatta
     2821 aatttacaaa tttagatgta aatgaatttt tgatagatga tagcatgata attaaacctg
     2881 ctgatgtagg aagtaatgta gaagtagtta gaggacctaa tataaaacct ttccctctta
     2941 atacagagct aagtcagtct ataggtggaa aagttatatt aaagacagaa gataatataa
     3001 ctactgacca tataatgcca tcaaatgcta agttacttcc ttttagaagt aatataccgt
     3061 atcttgcaaa ctattgtttc aatactgtag atacagaatt tcctcagaga gcaaaagaca
     3121 ataatggtgg atttatagtt ggtggagata attatggtca aggttcaagt agagaacatg
     3181 cagctttagc accattatat ctaggtgtta aaggggtaat agttaagagc tttgcaagaa
     3241 tccacaaggc taacttgata aatagtggta taattccaat ggagttttgt gatgagaaag
     3301 attatgaaaa tatatctctt ttagataatc tggagatacc aaatatttta gacaatctag
     3361 gtagtggaat tttagaagtt aagaatacaa ctaaaggaac ttcttttaag gtcaaagtag
     3421 aattgtcagc aaaagaagtt gacgtattaa aagctggtgg aaaattaaac tacactaaaa
     3481 atcaagctaa ttaatatgaa aagagggaat attgaatgta taaagtgaca cttataccag
     3541 gagatggaat aggaccagaa gttgcgaaag ctatgaaaaa ggtagtagaa gctactggag
     3601 cagaaataga atgggaagaa gttaatgcag gagaggcagt tattgaagag tatggtactc
     3661 cacttcctga atatattata gatagtataa aaaagaacaa aatagctata aaaggcccaa
     3721 ttactactcc tgtaggtaaa ggatttagga gtgtaaatgt aactttaaga caggcattag
     3781 atttgtatgt caatttaaga cctattaaaa gttttaaggg gataaaatca agatatgaag
     3841 atgttgattt agtggttgta agagaaaata cagaagattt atatgctgga atagaacata
     3901 aaataggtga ttatgcagct gaaagtataa agataataac aagaagtgca agtgaaagaa
     3961 tcgttgattt tgcatgtaac tatgttaaag ataataagag aaaaaaagtt acagctatac
     4021 ataaggcaaa tataatgaaa atgtctgatg gattgttttt agatgtgttt agagaagttg
     4081 catcaaaaca tggtgtagaa tatgatgatt tgattgtaga tgcagcagct atgaacttag
     4141 ttttaaatcc agaaaattat gatgtaatgg taatgccaaa tctttatgga gatatactat
     4201 cagatttagg tgctgggctt gttggaggtt taggtataat tccaagcgca aatattggta
     4261 aagattgtgc gatatttgaa gctgttcatg gaagtgctcc acaaatagca ggtcaaaata
     4321 aggctaatcc aacagcactt attcaatcat cagtaatgat gcttagatac ttaggtgaat
     4381 atgaaaatgc acaaaaaata gaaacagctt tagaaaaagt atttcttgaa ggaagtaaat
     4441 tgactgtaga tttaggtggc tcagcttcta ctacagagtt tgcggatgag gtgtgtaagt
     4501 atattgtata gattttattt agggaaaaag tgaaatatag attgaaggaa agaggctttc
     4561 tcaaaataga gataagcctt tttttattgg aatgataaaa tatatttgat tttatacaaa
     4621 agagagttcc tcataaacga aaaagttaat tttgggatat ccattttaat tttaaaatta
     4681 attgattaat acgcaaagaa agattaaaca aatttattat ttttaattga actttcaacc
     4741 aaaatatggt aaaatttaaa aaagttgaac tttcaactaa aaataaatat attgattatg
     4801 tgaaattcta taaaacagaa actatttgac tttagtcatg gtaggttcag tggaggtgtg
     4861 tttgattaag actttagata gtaatatatt aagagaagta ggtactttat ctagagcagt
     4921 aaattcaata aatgatatta agtacaagga attaaagtta caaaaaggac aatttacatt
     4981 tttgacaaga atctgtgaaa atcctggtat aaatcttgtg gaattatcaa atatgttaaa
     5041 ggtagacaag gctacaacaa ccaaggctat acaaaaacta ataaaggcag gatatgtaga
     5101 taaaaagcag gatgagtttg ataaaagagg atataattta actccaactg ataaatcttt
     5161 ggaagtttat gagttgataa ttgaagaaga gaatagaagt atagagatat gttttgataa
     5221 ttttacagat gaagaaaaac aagtggtaac taagttatta gaaaaaatga gcaaaaacgt
     5281 agaaaatgaa tggtttaaag taaagagata gtaaataaat acattaactt aaggagatat
     5341 ttttatgatt agaaaagcga atatgaatga tttagaaagc attatgaaaa ttataaaatc
     5401 tacagtggaa gaaatgaaga cttataataa tacacaatgg gatgaaaact atccattaga
     5461 aaaagacttt gtttcagata ttaagaaaca agatttatat atttatgaag tggatggaga
     5521 agtagcagga tttatatgtc ttaattatga agagccagaa gaatatttag gcttaaattg
     5581 gtcatctaat aaaaaagcaa tggtaataca tagaatggct gtaaatccaa actttagaaa
     5641 gacaggaata gcatctaagt tagttgattt tgctgaaaaa ttagctgtag aaaacaatgt
     5701 atcatactta aagagcgata catattcaat aaactcaaaa atgaatttat tacttacaaa
     5761 gtgtggtttt ataaaaatcg gagaaatgag ttttttagga aaggagaaat ctttctactg
     5821 ttatgataaa atattataaa gagcatttat aatacaaggg gtaaataata gattttcagc
     5881 attagtaagg gcataatata acccagtata tgctattatg aaagggatga tttatatgat
     5941 tggatttcta aagaaaagaa gaagtattag aaaatacaag gatgttgaag tagaaaaaga
     6001 aaaattagat aaaatattaa aagctgctct tttagctcca tcatcaaagg ggcttagaac
     6061 atgggaattt atagtggtag atgataaaga aaagctaatt aatttatctc aatgtagaac
     6121 taagggtggt ggatttttct taaaaaatgc acctttagct attgtaatta ttgcagataa
     6181 agaaaaaaat gatgtttgga tagaggatgc aagtatagct gcttcataca tacaacttca
     6241 agctcatgaa ctaggtttag gttcttgttg gatacaagta agaaatagaa tgtatgatga
     6301 taatatagaa gctgataagt acattagaga agaattaaaa gtacctagca agtacagtgt
     6361 agaatgtata atttctatag gatattctga tgaagaaaag aaagcataca atgactctga
     6421 tttagactat aaaaaggttc attttaacaa cttttagtat ataatatgtt gtattaagac
     6481 attatatttt tttctattgt taatatatac ttaaaattgt aaaaatatat ctgaatttta
     6541 ctaacttatc taggggttat gtacaaggat tttaaagata aatatagaaa ttaatgttta
     6601 taatcgatac ataacttgag taacaaaatt taaggaggtt aaagtatggg tatatttaaa
     6661 acaaaaatgg atgaggactg gaaagtaaac tacattaaag agtttaatga aatgagagat
     6721 tcttatgaga gtaaacttca aaaaaaacag tttgaggtag atagtttgaa atcagaattg
     6781 gatagactaa gaagctataa aaatagcctt aagccaaaag aaaaacaaat tactgatgat
     6841 gatataaata atataaagag tttaagaaga gatggcttaa gctataaaga aatttcaaat
     6901 caaacaagtt ggagcaaagc aactgtaagt agggtattaa atggacttta cgattagcat
     6961 atacatgggg tgatttaaat tgggatgaag tatagttaca aaaaatgcat aatagattct
     7021 attttattga tgtttataat tcaaatatta aggatgattt tgaattatgt acttctaagt
     7081 caatttgaat ttactcttga aaattttaat attataaact taatttcttt tacattagtt
     7141 ggtttatcgt taattttatt tttaaaagat aattctttgt ataacaaggt tagaaataga
     7201 aaaatcacag aagcttttga agaaaataaa aacaatatac ttattgaaaa gtgtaagctt
     7261 atactttttg ttgtggtttt atctttagca ataattgtaa cttattgtac taagggatat
     7321 gtacttttta atgtaactat gatgactttg tctgtattga tagttccaat atttgaagag
     7381 ttatttttta gagaatatat atggaactat ctaagtaatt ttataaagtc aaagagtaaa
     7441 attatctgta taacatctat actatctgga atttataata taggatacat agatgttata
     7501 agaaattatg taatattgta taacaattca agctacacct ttgaagttat tatttcaaaa
     7561 attatgatag gtactgtttt tggtattgta cttggattag ttaagtatag gtttagagat
     7621 gtagggtttt gtatattatt gaggagctta tttgctatat ttattaggta gatagtataa
     7681 tcggtttata aggttgtttt aaatttaatt ttaaatcaac cttattttat ttgtgtttta
     7741 taaa
//
LOCUS       DAMJVX010000160         7701 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMJVX010000160 DAMJVX010000000
VERSION     DAMJVX010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7701
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.166"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            4..87
                     /locus_tag="PC407_003004"
     tRNA            4..87
                     /locus_tag="PC407_003004"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38..40,aa:Leu,seq:tag)
     gene            111..185
                     /locus_tag="PC407_003005"
     tRNA            111..185
                     /locus_tag="PC407_003005"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:143..145,aa:Gly,seq:gcc)
     gene            210..286
                     /locus_tag="PC407_003006"
     tRNA            210..286
                     /locus_tag="PC407_003006"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Arg,seq:tct)
     gene            296..371
                     /locus_tag="PC407_003007"
     tRNA            296..371
                     /locus_tag="PC407_003007"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:329..331,aa:Gln,seq:ttg)
     gene            380..455
                     /locus_tag="PC407_003008"
     tRNA            380..455
                     /locus_tag="PC407_003008"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:413..415,aa:Lys,seq:ttt)
     gene            458..546
                     /locus_tag="PC407_003009"
     tRNA            458..546
                     /locus_tag="PC407_003009"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:492..494,aa:Ser,seq:tga)
     gene            550..625
                     /locus_tag="PC407_003010"
     tRNA            550..625
                     /locus_tag="PC407_003010"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:583..585,aa:Phe,seq:gaa)
     gene            632..705
                     /locus_tag="PC407_003011"
     tRNA            632..705
                     /locus_tag="PC407_003011"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:666..668,aa:Met,seq:cat)
     gene            720..796
                     /locus_tag="PC407_003012"
     tRNA            720..796
                     /locus_tag="PC407_003012"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:754..756,aa:Ile,seq:cat)
     gene            814..890
                     /locus_tag="PC407_003013"
     tRNA            814..890
                     /locus_tag="PC407_003013"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:848..850,aa:His,seq:gtg)
     gene            899..974
                     /locus_tag="PC407_003014"
     tRNA            899..974
                     /locus_tag="PC407_003014"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Lys,seq:ttt)
     gene            982..1055
                     /locus_tag="PC407_003015"
     tRNA            982..1055
                     /locus_tag="PC407_003015"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1014..1016,aa:Cys,seq:gca)
     gene            1063..1139
                     /locus_tag="PC407_003016"
     tRNA            1063..1139
                     /locus_tag="PC407_003016"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1097..1099,aa:Arg,seq:acg)
     gene            1152..1227
                     /locus_tag="PC407_003017"
     tRNA            1152..1227
                     /locus_tag="PC407_003017"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1185..1187,aa:Val,seq:tac)
     gene            1303..1716
                     /locus_tag="PC407_003018"
     CDS             1303..1716
                     /locus_tag="PC407_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498429.1"
                     /translation="MYIVIGNEIVDSEELKIIIDENSKFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLSSLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2025..5456
                     /locus_tag="PC407_003019"
     CDS             2025..5456
                     /locus_tag="PC407_003019"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDF2498430.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5698..7626
                     /locus_tag="PC407_003020"
     CDS             5698..7626
                     /locus_tag="PC407_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2498431.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3035 a          962 c         1544 g         2160 t
ORIGIN      
        1 tgcgcggatg tggcggaatt ggcagacgca ctagacttag gatctagcgc cattggcgtg
       61 ggggttcgac tcccttcatc cgcaccattt ttatttaatt taataaacga gcgggaatag
      121 ttcagtggta gagcgcaacc ttgccaaggt tgaagtcgcg agttcgaatc tcgtttcccg
      181 ctccaaattt tatgtttatg ttgtaatgtg ggtgcatagc tcagctggat agagcaacgc
      241 ccttctaagg cgtgtgtccg gggttcgaat ccctgtgcgc tcaccacata tatattgggg
      301 attagccaag tcggtaaggc acatgacttt gactcatgta tgcgtaggtt cgagtcctgc
      361 atccccagcc aaacagtatg gtctattagc tcagtcggta gagcacctga cttttaatca
      421 gggtgtcccg cgttcgagtc gcggatagat caccaatgga gaggtgtccg agtggtttaa
      481 ggagctggtc ttgaaaacca gtgacttcga aaggggccgt gggttcgaat cccaccctct
      541 ccgccaaatg cccagatagc tcagtcggta gagcagggga ctgaaaatcc ccgtgtcggt
      601 ggttcgattc cgcctctggg caccaaaatg tggcggtata gcttagttgg ctagagcgtt
      661 cggttcatac ccgaaaggtc ataggttcga ctcctattac cgctactaaa atttaatgtg
      721 ggccattagc tcagttggtt agagcgcccg gctcataacc ggtaggtctg gggttcgagt
      781 ccctgatggc ccaccacatt aaattaactt atggtgggta tagctcagtt ggttagagcg
      841 ccagattgtg gctctggagg tcgtgagttc gactctcatt atccacccca acttatatgg
      901 tctattagct cagtcggtag agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg
      961 cggatagatc accaaaaagg cggcgacata gccaagtggt aaggcagtgg actgcaactc
     1021 cttgatcccc agttcgaatc tgggtgtcgc ctccaataaa atgcgctcat agctcaactg
     1081 gatagagtgt ctgactacga atcagaaggt taggggttcg agtccctttg ggcgcaccat
     1141 atgaatagcg agggattata gctcagctgg gagagcacct gccttacaag cagggggtca
     1201 caggttcgag ccctgttaat cccaccagtg caaacctcta gtaaaactac tagaggtttt
     1261 atttttgatt gaaaaagttt ttatatttag gaggtaagaa atttgtatat agtaatagga
     1321 aatgaaatag ttgatagtga agaacttaaa ataattatag atgaaaatag taaatttaaa
     1381 gttgaaaaag atttaagtaa aagcacaaaa agagaagatg taatagcata tcagttaagt
     1441 atagatttaa attacctaga ttctcttata aatgagcaat gtaatttgtc cagtttatct
     1501 gatgaagaga aatttgatga atatatgact ttgtctgatg aacttgcact ggatttagaa
     1561 gagttaatgc caaaatatac aataataaat gctagagcat ataaattaga cgaggtagat
     1621 ggaattgtaa aaataatact agctgtagcg tatgcagatt tagggcactt aaaactttct
     1681 gatgtagtaa agaggctatc tagacaagta gattaggata tgaatatgaa ctatattaaa
     1741 aaaatgtata aaatcttacg taaagatgaa aataattatg taaacataga aaaaaacagt
     1801 ataaaatcaa gtcaattgta gaaattttca cataaattca tctgaaattc tataaaaaat
     1861 gccttaattt taatgaggta aatcgagttt acacagtcta aaaattactt atatttataa
     1921 atttatctta ttaattaagg aactttctta aacatcttca aaaataaagt attttagtct
     1981 ataatcttta atattacaca aacaataaag gagaagaata gagtatgctt aaaaagttta
     2041 ataaaatact agtggctaat agaggcgaaa tagctataag aatatttaga gcctgttcag
     2101 aattggggat aaaaagtgtt ggtatataca gtaaggaaga taagtatgga ttgtttagaa
     2161 caaaagcaga tgaatcatat ttgataggag aaggtaaagg gccaatagat gcatatttag
     2221 atatggatgg aataatagac ctagcaaaga gaaaaaaagt tgatgctata catccaggat
     2281 atggttttct agcggagaat gcagaatttg ctcgtaaatg tgaagagaat ggaattactt
     2341 ttataggacc atcatctaag gtaatgaata tgatggggga taagattaac tctaaaaaaa
     2401 tcgcaaaaga agttaatgtt caaactatac caggagtaga aaaagcaata agaagtactg
     2461 aagaagcaaa ggaagttgca aacaaaatag gatatcctgt tatgataaaa gcatctaatg
     2521 gcggtggcgg tagaggtatg agaattgtac atcgcgaaga agacttagaa cttgaatatg
     2581 aaactgcttg tagtgaatca agaaaagctt ttggagaaga tattatattt atagaaaaat
     2641 atatagctga tccaaaacat atagaagttc aaatattagg tgataattat ggaaatatag
     2701 ttcacttata tgaaagagat tgttcagttc aaagaagaca tcaaaaaata atagaatatg
     2761 caccagcttt ttcgttagat gataaagtaa gaaaagagat atgtgaagat gcagttaagt
     2821 tatcaaaaca tgtgggttat tctaatgcgg gaacattaga atttttagta gatgcaaatg
     2881 gtgggcatta ttttatagag atgaacacaa gagtacaggt tgaacatact gtaacagaga
     2941 tggttacagg aatagatata gttcaaagtc aaatactaat agcccaaggt tacagcttag
     3001 atagtgaaga aattaatata aaatcacaag atgatgtgga aataagaggt tactctatcc
     3061 aatgtagaat aactacagaa gaccctaaga ataaattcat gcctgataca ggtaaaattc
     3121 aagtttatag aactggttca ggatttggta taagactaga tggaggaaat ggatttacag
     3181 gggcaaacat aagtcctcat tatgatagtt tattggttaa gacaatatct tgggatagaa
     3241 cattccaagg agcaataaat aaaactataa gatctataaa agaacttaga gttagaggtg
     3301 ttaaaactaa tgtaggattc ctagttaatg ttttaaataa tcctatattc tcaaatggta
     3361 aatgtagtac taagtttata gatgaaaatc cagatttatt tgaaataaca gaaagtaaag
     3421 acagaggaac aaaattactt caatttatag gagatgtaat tgtaaatgat aatgcttgta
     3481 aagaaaaacc actatttgat gcattacatg acccaagaat ggataaggat ggttcaaaat
     3541 cagaaggaag taaaatactt ttcgataagt taggtaaatc tgcatatata gaaaagataa
     3601 aaaatgataa gaaattacta ttaacagata caacaatgag agatgcacat caatcattat
     3661 tagcaacaag aataagaaca tatgacttat taaaagctgc taagccaaca gaaaaatatc
     3721 aaaaggattt attctcactt gaaatgtggg gaggagctac ttatgatgtt gcatatagat
     3781 ttttaaaaga atctccttgg cgtagattac aaaagctaag agaagaaata ccaagcataa
     3841 tgttccaaat gttgcttaga gcatcaaatg gagttggata taaaaactat ccagataatg
     3901 taatagaaga atttacaaaa gaatctgcta gacaaggtat agatgtattt agaatatttg
     3961 attcattaaa ctgggttgaa aatatgaaac catctattaa tacagcttta gaaactggaa
     4021 aaattgttga agcaactatg tgttatacag gtgatatact agataagact aaaactaaat
     4081 acaatctaga atattatatt aaaatggcac aagaattaga aagcttggga gcagatataa
     4141 tagcaataaa agatatgtca ggtcttctta aaccatattc agcttacaca ctagttaaag
     4201 agttaaagaa aaatgtaaaa gcaccaatac atttacacac tcatgataca agtggaaatg
     4261 gtgtagcaac ttgtttaatg gcgtcagaag ctggagtaga cataattgat gctgcattag
     4321 aatctatggc aggacttaca agtcagccat cgcttaatgc tatagtagag gcacttaaga
     4381 atacagagag agatacagga atagatttat ttggatatga tgagttagga aaatattata
     4441 aggatttaag aaaagtttat aataaatttg aaagtgattt aactaattct tgtgcagaaa
     4501 tatataattt tgaaatacca ggaggccaat atacaaactt aaaacctcaa gctgatagtt
     4561 taggattagt taatagattt gatgaagtta aagaaaaata taaagaagct aatgaagttg
     4621 taggagatat aataaaagta actccatctt caaaagttgt aggagactta gctatcttta
     4681 tgactaaaaa taaattagat aaagacaata taatagaaga aggtaaaaat ttatcattcc
     4741 cagattctgt tgtggattac tgtaaaggta tgattggtca accagaaggt ggaataccaa
     4801 aagatttaca agaggtagtt ctaaaaggtg aagaagctat aacagtaaga ccaggttcat
     4861 tattaccagc agaagatttt gatgaaatag ctaaatatct aaatgaaaag tatgatataa
     4921 atgctaatat aagaaatgtt ataagttatg ctttatatcc aaaagtatat gaagattata
     4981 tcaaacatct acaacattat aatgatatat ctaaacttga aagtgatgta ttcttctatg
     5041 gattaaataa aaatgaagaa tgtgaagtag aaatagaaga aggtaaggta ttaacaataa
     5101 gattagttga aattggtgaa gttaaggaaa atggatttag aactatagga tttgagttaa
     5161 atggaatggt tagagaagta gaaataaaag ataagaattt ctctggaaaa attaataatg
     5221 ttgaaaaagc tgatatgaat gaccctcttc aaattggagc aagcatacca ggaaaagtta
     5281 taaaaataat ggttaaagaa gaagatgaag taaaagcaaa tcaaccatta atcgttatag
     5341 aggctatgaa gatggaaact ataatagttg ctaagactga tggagtaatt aagtcaatca
     5401 aagtaaaaga agatgatatg gttgaagata aacaattatt aatgataatg aagtaattta
     5461 attaaaataa attaaaaagc actctgaaat gtttaataaa aaatgtttta gagtgttttt
     5521 atttatttta attaaatatt tgttaaaaaa aagaggacaa ttacctccag atgtagaaat
     5581 aaagctagta aggaaaatgt tatctaaaaa ttctaaatat agtaaatatt ttccataata
     5641 tttcttagga aataacagca aagataaaat acataaattt aaaaggtggg tgcttatatg
     5701 aaggtttatg atagtaaaat gttgagaaat gtagcagtat taggacatag cggatgtgga
     5761 aaaacaaatc taatcgaaac tatagcttac acagcaaaca ctaataagat acctaaatta
     5821 acagacaagg taaatatgac ttatagcatg ggattaatac ctatagagta taatgattat
     5881 aagtttaact tattggatac cccaggatat tttgacttta gcggagacgt tgtgtcatct
     5941 cttagagcaa gtgatgcagc aataatagta atagatgcaa cagcgcctat tcaagttggt
     6001 acagagaaat cattagaact aactgagagt attcctaaga taatgtttat aaataaaata
     6061 gataatgaaa aggcaagata caaagatgca atagcaatgt taagagaaaa atacaataat
     6121 aagatagttc ctatgataag tcctatatac aaagataaaa actttgtaaa gttacataat
     6181 gtttttgaaa acatagatga tttagaaggt gaatttaagg aacaagctat gagtgttaaa
     6241 gaagcattaa tggaacttat agcagaaact gatgaccaaa tattagataa atattttaat
     6301 ggagaagaac taactacgga agagatacaa aaaggtatta ttataggtat acaaagaggt
     6361 gatattatac ctgtaatatg tggttcaact ataaataata taggcacaaa ggaaatatta
     6421 gatacaatat caagttattt agaacctatc tttacagagg aaagcaaacc atttagggga
     6481 ttagtattta aaactatggt tgacccattt gttggaaaga tgtcatatat aaagattact
     6541 gagggtgtac tttctaagga taaagatgtt tttaatataa ataaaaatgt aaaagaaaaa
     6601 atagctaata tatatacatt gagaaatagt gaattagtag aaatagaaaa agctaaagct
     6661 ggtgatatag tagttataac taaagttaat tctctaaaaa caggagacac tatatctgca
     6721 gataaagatg cagaagcttt agaaaagata gattttccta aaccacaaat atattatgca
     6781 gtaactccta agaataaagg tgatgaagaa aaagtagcat ctgtattaaa taaactagta
     6841 gaagaggacc ctacattaca ttggtataga aatacagaga caaaacaagc acttttagga
     6901 ggtcaaggag aacttcatat aaaaacaatt aaaaataaaa tgaaagataa gtttggtgtt
     6961 gatgttgaat tgaatgacct taaagttcct tatagagaaa ctataaaagg aacagccgat
     7021 gtacaaggaa aacataaaaa gcaatcaggt ggtcatggtc aatatggaga cgtaaaaata
     7081 agatttgaaa gatgtgaatc tgattttgaa tttacagaag aaatatttgg tggttctgta
     7141 ccaaaacaat atataccagc agttgaaaaa ggtctaaaag actctatgca aaaaggtata
     7201 ttagcaggtt atcctgttac aaacataaaa gctacgcttt atgatggttc ataccatgat
     7261 gtagactctt ctgaaatggc ctttaagatg gcagcaagtg cagcctttaa aaaaggtatg
     7321 gaagaagcgc acccaatact actagaacct ataatgaaac ttaagataac tgttccagaa
     7381 gaatatatgg gagatgtcat gggtgatata aataagagaa gaggaaaaat atttggaatg
     7441 gaaccagatg acaaagggaa acaaataata tttgcagaag ctccacaagc agagacattt
     7501 aaatatgcaa tagatttaag agcaatgaca caaggtagag gatactttga aatggaatta
     7561 gagagatatg gagaggttcc atcacagttt gcagaaaaaa taattgggtt agctactgca
     7621 aagtaatgaa caaaataaat agaaaaataa ttaatatata ttattaaaaa tatttttagg
     7681 gttttaacta ggtataggtt t
//
LOCUS       DAMJVX010000161         7699 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMJVX010000161 DAMJVX010000000
VERSION     DAMJVX010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7699
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.167"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            65..1663
                     /locus_tag="PC407_003021"
     CDS             65..1663
                     /locus_tag="PC407_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2498432.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIIW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            1858..2658
                     /locus_tag="PC407_003022"
     CDS             1858..2658
                     /locus_tag="PC407_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2498433.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(3569..4078)
                     /locus_tag="PC407_003023"
     CDS             complement(3569..4078)
                     /locus_tag="PC407_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2498434.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDSLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVIVIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            4900..5337
                     /locus_tag="PC407_003024"
     CDS             4900..5337
                     /locus_tag="PC407_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HDF2498435.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            5476..6264
                     /locus_tag="PC407_003025"
     CDS             5476..6264
                     /locus_tag="PC407_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2498436.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            6309..6767
                     /locus_tag="PC407_003026"
     CDS             6309..6767
                     /locus_tag="PC407_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498437.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(6861..7211)
                     /locus_tag="PC407_003027"
     CDS             complement(6861..7211)
                     /locus_tag="PC407_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498438.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(7213..7596)
                     /locus_tag="PC407_003028"
     CDS             complement(7213..7596)
                     /locus_tag="PC407_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2498439.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
BASE COUNT         2816 a          962 c         1205 g         2716 t
ORIGIN      
        1 ttacttaaaa ggttgtatat aaataatttc aaatatatat cctaatttat aggaggtttt
       61 aattttgaaa gctgcaattt attcaagaaa atcaagattt actggaaaag gtgattctat
      121 agaaaatcaa atacaattat gtattgatta tgcaaagtct attggtgtaa aagatttttt
      181 gatatatgaa gatgagggat ttagtggtag caatatagat agaccacaat ttaagaaaat
      241 gatgcaggat gctaaagata agaaatttag ttacctagtt tgttatagat tagataggat
      301 atcgagaaat gtatctgact tctctacttt aatagaaaaa ttaaatgaac taaacatatc
      361 atttatatct atcaaagaac aatttgatac ttcaactcca atgggaagag ctatgatgtt
      421 tatatcaagt gtatttgctc aattagaaag agaaacaata gcagaacgta taaaagacaa
      481 catgtatgaa ttagcccgta gtggtagatg gcttggaggc aatacacctc atggattcaa
      541 cagtgaaaaa atcacattct tagatgaaaa tctaaaagaa agaagtatgt ataagcttca
      601 agtaaatgat gaacaaatgg agattgtaaa gctaatatac aataaatatt tagaacttag
      661 gtccttatct aagttatata aatatgtcta taataatgga ataaggggtc ctcgtggtgg
      721 taaatttgac cctagttctt tatctagaat acttagaaat ccagcatatg taaaagctaa
      781 tgatgaaatt ttagattacc ttaaaaatag tggtatggat gttgtaggta ctccagatag
      841 aaaatgtgga atccttacct atgctaaaaa tacatctaac tcaatagcag caattgcaaa
      901 acaccatgga gtcatagaac ctgaaatatg gcttgaagta caaaatcaat tagataaaaa
      961 tcgtgataaa actccaagga tatcaactgg aaaaactgct cttttatctg gtcttataaa
     1021 atgtggtaaa tgtggttcta atatgagaat tacctataaa ggcaaaaaaa ctgacaagga
     1081 tttaaagtat tattatattt gtggtactaa aaaaacactt gggtctgata gttgcaactg
     1141 taaaaatctt aatggtccat tagttgaaag tttagttata gataaaatta agaattgtaa
     1201 agaagagtct attataagtg cattcaatag taataacatt aagactgata atgtttttaa
     1261 tatttttaaa tctgagacta atttacttaa aaatcaaatt ttagataagg aaagacttat
     1321 taaaaattta gttatagagc ttgcaaaaaa tactggtagt actgcttctg attatatagt
     1381 ttcccagatt gagagcctaa ataatgagat tagtatttta aaggataaga ttaatatttt
     1441 ggaatctagt aatatcaata ttagtagtga cagtattaat attgatatta ttgttagtaa
     1501 tttgaagaga tttaatgatg aagttgataa tgccagttta gatgataaga gattgttatt
     1561 atctactatt attaatagta tcatttggga tagcagttta ggaaatctta atattgttta
     1621 tatgggtgct gaagtggata attctgtttc tttaagcaat tagttgcact tttttagcta
     1681 tagcagaagc aataagcata tcagataagg ttgcaatact atctaaaaga ccagctacca
     1741 taaaaaatat ttacgatatt gaacttaaca tggatggaac aaaaacgcct ctcaaaacac
     1801 gtaaagttcc tgattttcaa aagtatttcg acatattatg gaaggagcta gatacacatg
     1861 aggcttaata aaagatctga aggatatata aactatgtta aaggtgtaaa aagagaaaaa
     1921 agaaatgtat tattctacca gctattaatt ttaattggtt ttattgtaat ctgggaactt
     1981 ttagctgact taaatgttat aaatacattt ctatttagca aaccaagtga tatttacaat
     2041 ttatttattc aatatgcatt tagtggtcaa ttatttaaac atattggaat atctgtatat
     2101 gaaactgttc ttggtcttgt tataggtact gtattaggta ttttggtagc aattgctctt
     2161 tggtggtcag aaaaattatc aaaaatatta gacccatttt tggtagtttt aaatgctctt
     2221 cctaagactg ctttagctcc tataattata gtttgggtag gtgctggaat agaaggaatc
     2281 gtcgtaacag ctgtaacgat ttctgttgtt gttactatac tttcagctta taattatttt
     2341 atgaatatag atgaagaaaa aattaagatg ttaaaaagct ttggagctac taaatctcaa
     2401 atattattta aactcatatt acctgcaaac acaggaaatt taataaattt aactaaaatt
     2461 aatattggta tggcatgggt aggtgtaata gttggagaat ttttagtttc aagatatgga
     2521 ataggctact tgatagtata tggttctcaa gtatttaaat tagatttagt aatgatggga
     2581 gtatttgtat tggctatttg tgcttgggca atgtacgctg tagttaatat tatagaaaaa
     2641 atatacaatt ccagataaat aaaaatttta tttagaatat ttgatgtatc agtgattata
     2701 ataaaaaatg atttctttac agtaatcatt tttttaaatt aaaataaagc gtaagctttt
     2761 gattatcttc ttataactca tcatgttgct agcacagctc tattttatgg gctttgctgg
     2821 ctttttattg cctccaatta tatacataat gcttaaatgc agtcatttct ttattaccca
     2881 tgcttaaatt tataaaaaag aaaagacagt caacttttta atcaaaatta actgtgcttt
     2941 atatttaata tttaattttc atttttgtgc tccgtttgtt agatagcaga gacttaattt
     3001 tgtattttga acaggattga atcttaaaaa tttcccaatt atatcaattt caaagtccat
     3061 taagaataat cctataagaa ctttgctaat ttgatctaag aatattaagg atataactgg
     3121 aaaaacaaaa gactttattt gcctgatatt aacacctcct tgcaaaagtc attcactttt
     3181 tccttactca gtattgttaa atctaaaaaa ggaatttcga tttattaatt tcacatcaat
     3241 aattataaca tgagtaaatt atcaagtcat ttcttttctg atttcgtaga taatacttat
     3301 ctccatatat ttctactcgt agtcgttgta caaatcgttg taaatcatgc tacagacctc
     3361 gcattttctg catctgacgc atgagagtat aagtccagca gtcttgcttc ctgtacttta
     3421 cttaatgcgg ttttgggcct actggcgtcc tcaataactt cgcttagttg gaaatcaaaa
     3481 aggttcttcc ttacatagtt gtcctcgatt gccatgtaga atgacacttt taaggaacgc
     3541 ttgctatatt ataaaaagca gagttacttc atattttctt acgtgtcaaa aagattccaa
     3601 taattgctcc tataaggaca atcggtacta atctgacaaa tagccactca aaagcatgaa
     3661 aagctactcc aaaaacagcg gtttcagggt cggtataatt ccaatccaag taaggactat
     3721 ttagtccccc taagaccaca aaaactatta ctatgactgc acataataaa ataaataacc
     3781 aatgaaacat ttttcttctc atgttttttc tttgtgcaag ttgcaagttt ataatctcta
     3841 atttttcaga aatcactttt aagctatcaa cttccgactc aacaatagtt tctccaagta
     3901 aagtgcttac aggcgtttca agcacctctg atatggaaat caacatatca gaatcgggaa
     3961 ccgacagtcc ttgttcccat ttagatattg tttgtcgcac tacattcagt ttgatagcaa
     4021 gttcttgttg tgaaagtcct tttgacttcc tgattgtttt aatgttttcg cttaacatta
     4081 gggctaacca cctttctttc agttgttacc gatattatag gcaaaactgc ccgttgcttc
     4141 aagcaacgca ttttaacatt caagtaaatt tcttcttttt cctttaatcg ttttgcttat
     4201 atacagcttt tacgcggata ttctcgattt ttaagcgctt tttataataa aaatagatat
     4261 gtccagtaat taaaactgta gaaatattta taaaggcata agacaaattt acctacaatt
     4321 tttagtgttt agattctaaa aaaataaaaa taattataat tgagttcata attttaaaac
     4381 ttattgaata taattatatg tcggttaaag acgtatgaaa ataaaaggaa tttacaagtt
     4441 atgtgttgta tagatattgt atatgcagca tgtaataaat cttagcacta tttatattct
     4501 atacaaaaag atatttgaag ggagattaaa aatgggaata ttagaaggcg ctgcaatgat
     4561 aagagagatt gcaattaaaa tagctaaaga aaaaggtatt acagaacaaa aggcatggcc
     4621 cgaagctgta aaagaattta aagaaaaata tgaacttgta ttataaactt tgactgtaaa
     4681 actgtctcta ttttattgag acagtttttt atattgtaac attcatatta cgaagaaaga
     4741 tatatataat tttagattcc ttgtaacttt aatttaaaat gaaataattt agactaaaaa
     4801 cagtattaat aaattttatt tatcgtataa atttgttttt aatattattt ttaaggtata
     4861 aatcttatat attagatata aattttggag gtatatgatt tgaataaaaa acttgaaaat
     4921 agatgctcat ggcaagaata ttttatgaga ctgtgcgaaa cagtagctga aagaggaact
     4981 tgtgatagag cttatgtagg agctataatt gtcaattcag aaaacagaat agtgtctact
     5041 ggatataatg gtagtatttc tggagataag cattgtagtg aagttggtca tgaaatgaga
     5101 gatggacatt gtattagaac cattcacgct gaacaaaatg ccctttatta ttgtgcaaaa
     5161 gaaggtattt cagttaaaga ttgctctatt tatgtaactc actttccttg cttaaattgt
     5221 actaaggcta taattcaagc tggaattaaa catatatatt atagaactgg atatagaata
     5281 gatgaatatg ctataaaatt attgcaatca agtaatgtct tatatacaaa attataattt
     5341 taacattatt tacttaatat tctatatttt tacaaaatgg gttataataa aagtataaga
     5401 tataaaaaga aacaaaattc taggttatgc atatattaaa tagaagttaa atttaattgt
     5461 aaaggagatg ttagtttgaa agtagtaata ataccagggc acactttaat tggaaaaggt
     5521 acaggagctg ttgggtatat aaacgaaagc aaagaaacaa gaatactgaa tgacttaatt
     5581 gttaaatggc ttaaaatagg tggagctact gtttatacag gaagagttga tgaatcaagt
     5641 aatcatttag cagaccaatg tgctatagca aataaacagg aaactgattt ggctgttcag
     5701 attcatttta attcaaatgc tacaacttca actccagtag ggacagaaac aatatataag
     5761 actaataatg gcaaaaccta tgcagaaaga gtgaatacta ggttagctac agtattcaaa
     5821 gataggggtg caaagtctga tgttagaggt ctttattggc ttaatcatac tattgcacca
     5881 gcaatattaa tagaagtatg ttttgtagat agtaaagcag atacagatta ttatgttaat
     5941 aataaagata aagttgctaa attgatagca gaaggtatac taaacaaatc tatatcaaat
     6001 tctcaagggg gaggggaaaa taaagtgtat gaaaatgtaa tcgtttatac aggtgatgct
     6061 gataaagtgg ctgctcagat tttacattgg caattgaaag attctttaat aattgaggcg
     6121 tctagttata agcaaggttt aggtaaaaag gtttatgttg taggtggaga agctaataaa
     6181 cttgtaaaag gtgatgtagt aataaatggt gctgatagat atgaaactgt taaattagca
     6241 ttacaagaaa tagataaatt ataaaatcat gattatttta taaaaaataa ataagttagg
     6301 ataaaaaaat gataagaaca aaagttgtag agttgatagc aacagtatgc agagaaaaca
     6361 aaccacataa atgggttgat gaaaattata ctccttatga taaaagtggt aaagttgaat
     6421 taatgtcaat agaagattta aatgaattaa tatcaagcag tggaagagca gattttcttt
     6481 atagcagtag attacaaaag cttctaaatg aggtttatat aaatcaaagt agagcatctt
     6541 atatatcagg ttgtggatta ttttggagtt cgtattggga tatacttgaa gaaaaatttg
     6601 aagaatggct atataattct tatatctttt ttgatgaaga tgatgaatat ttagaaggaa
     6661 tggaagattt tgagcttgaa tgtaaagatg ttcttatgga tgtaattgaa acaacaagca
     6721 tagatatata tgttcaaatg ataaagagga atattaccaa ttattaaaaa cacatagtgt
     6781 caaatcaagc taataaaaaa cgaccttata aatgttattt taaggtcgtt taaattatat
     6841 cttattttat attaaattta ttacttttta tctagaaaaa tatttttaat tatagtcaat
     6901 atatcgacta taacatccat atttgatttg acactgtctt tagtaacaag aaaatctgct
     6961 gtaacttcag taactacttc tgttacatct ttcatatttt ctcctacttc tccaaaagtt
     7021 tctacaacat taggaagatt atttaaagtt gtatttatat tttgagtatt ttcatttaac
     7081 atagttccaa cattatttaa aatcttatgt agtttaacaa gtacaataat taaataaact
     7141 aaagcaatac aagcaagtat agctaataat acatatatta tacttatgtt aatcgttatt
     7201 gtattgtcca tattattcct cagtaacact aactggttca actatatcta ttattttatc
     7261 ttcctcacta gaattttcaa cactcttttt gttatctata tattttttta tttttttctt
     7321 tgattctata aagtttcctt ttttatcctc tagcttctca gatactttgc cttttacatc
     7381 tacagttttc atatttatct tttcagcaat ttgttgtgaa gcatctttta tatcctctct
     7441 tgtttctttt ccagatttag gagccaaaag tactccactc actgcaccag taacagctcc
     7501 taataccaca ccagctgtag caattttagc tttcttaact ttttcagctc tctgtttagc
     7561 ttttctttta tcttctattt tcttcgatag attcatgttt aatcgctcct tattataata
     7621 tttctaaaca ttataataca ttctttacca taatgatata gtttctaact aaatttttaa
     7681 aaatcaataa aaattcttt
//
LOCUS       DAMJVX010000162         7691 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMJVX010000162 DAMJVX010000000
VERSION     DAMJVX010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7691
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.168"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(<1..1315)
                     /locus_tag="PC407_003029"
     CDS             complement(<1..1315)
                     /locus_tag="PC407_003029"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDF2498440.1"
                     /translation="MINIEIPKKVDYIIKELETNGYEAYVVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLISTEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILRRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGHILNKMLEIVINNPELNEKEILKEK"
     gene            complement(1466..3271)
                     /gene="lepA"
                     /locus_tag="PC407_003030"
     CDS             complement(1466..3271)
                     /gene="lepA"
                     /locus_tag="PC407_003030"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDF2498441.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            complement(3553..3804)
                     /locus_tag="PC407_003031"
     CDS             complement(3553..3804)
                     /locus_tag="PC407_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498442.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIIGTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            complement(3835..4854)
                     /locus_tag="PC407_003032"
     CDS             complement(3835..4854)
                     /locus_tag="PC407_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HDF2498443.1"
                     /translation="MFKKCIKVVTLTFILACILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEENTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            complement(4872..5825)
                     /locus_tag="PC407_003033"
     CDS             complement(4872..5825)
                     /locus_tag="PC407_003033"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HDF2498444.1"
                     /translation="MINVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            complement(6168..6944)
                     /locus_tag="PC407_003034"
     CDS             complement(6168..6944)
                     /locus_tag="PC407_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2498445.1"
                     /translation="MKFHEFGDKNKPHIMLIHGGGNAWWNYLRQAEVLSNNYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKM
                     FQSYVPSCEIYEAKGYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            complement(6978..7499)
                     /locus_tag="PC407_003035"
     CDS             complement(6978..7499)
                     /locus_tag="PC407_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2498446.1"
                     /translation="MDIAKLILNPARLRILQYIRLHGSARTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVVDNDKTK"
BASE COUNT         2361 a         1384 c          873 g         3073 t
ORIGIN      
        1 ctttttcttt tagtatttct ttttcattta attctggatt atttatgact atttcaagca
       61 tcttatttaa aatatgtcct atatctttac ctggttttat acctatagat atcaaatctt
      121 ttccattaat agctaaatct ttaagattaa agcactcctt ttgatttatt atatgtttaa
      181 attctttttc acactcaata agatttaata tcttttgttt tgcatacttt gggtttttgg
      241 caagtgtatc agcccaatta acttttatta aatcttcaaa taattcaaca tcttcaagtt
      301 tatttaataa ctttttaatt atcttccttt taggttctat tctataatca tgatattgta
      361 ttaatattaa aactttatta atagtataat tatcatactt aagtcttctc aaaatcttct
      421 ttgcaatctt tacagattct ctactatgag tataaaaatg gtcaacacca tttttatcag
      481 ttgttttagt tactttttta cctaaatcat gaaacaacat tgtaagtttt aaatacagtt
      541 catcttcaat tacttctgta gatattaaag tatgtttttg tacatcatag atgtgatatg
      601 gattgtgttg agtaacatct tctaattcac atatttcagg aattataaat ttcaacaaac
      661 ctgttgattt taataagttt aatttagatg aatcagatac taatatttta ttaaactctg
      721 tctggattct ttcgatagaa acatttttta tcaaatctgc ttttttatga atactttgct
      781 tagtaccttc agctatttta aaattaagtt gtgctgaaaa tctaactgcc ctaagcattc
      841 taagtgcatc ttcttcaaat ctatctactg gatttccaac acaacgtata tacttatttt
      901 gtatatcttc atagccatta aatgggtcga ctaatcctgt ctttgaattg tatgcaattg
      961 aattgatagt aaaatctctt cttgacaagt ccttaactat gttatttgta aattctattg
     1021 aatctggatg tctaccatca ctataatttc catcaattct ataagttgtc acttcaaatg
     1081 gttcatgttc aatcataact gttacagtcc catgttgtat accagttgga attgtttttt
     1141 caaataattc tactaccact tctggtctag cactagttgt tatatcccaa tcgtttggaa
     1201 ctctttctaa taaacaatct ctaacgcatc ctccaactac ataagcttca taaccattcg
     1261 tttctaattc tttaattata tagtctactt ttttaggaat ttctatatta atcataaata
     1321 tcaccttttt attcttcttt aatatattct tacttatatt ctctatgagt ttacttaaaa
     1381 tataccacaa aaaagctaga ttaatttaga aataattcta aaatcactct agcttttaaa
     1441 tatatttatc taaatttata agaatttatt catctagttt aagtacagac ataaatgctt
     1501 tttgaggaac ttcaacagaa ccaatttgtc tcatacgctt cttaccttct ttttgctttt
     1561 caagaagttt cttcttacga gatatatccc caccataaca tttagcaaga acatcttttc
     1621 ttagagcgct tattgtttct cttgcaataa ccttattacc aacagcagct tgtataggta
     1681 tagcaaactg atgtcttgga atttctcctt ttaatttctc acacattgcc tttcctctag
     1741 ggaaagcacg tgtctcatgg acaataaaac taagtgcatc cacttgttct ttattgatta
     1801 atatatcaag ttttacaagt gtagatgcta cataaccttt aacctcataa tcaagagaac
     1861 cataacctct agttctcgat tttaaagcat caaagaaatc gtaaacaact tcattgagag
     1921 gaaggtcata gtgaagcatt actcttctct catctatata ttccatattt aacatatttc
     1981 cacgtctttc ctggcaaagc tccataacaa cacccacata gtctttagga actattatat
     2041 ctccttttac tataggctct tctatcatct ttatctcaga tggttctggt aagtttgcag
     2101 gattttgtat cattacaacc tctccatcca tttttgtaac tctatatata acagatggag
     2161 ctgtagtaat tatatcaagg ttaaattctc tctcaagtct ttcttgcatt atctccatat
     2221 gtaaaagacc taagaaacca catctaaaac caaatcctag tgctgctgaa gtctcagctt
     2281 catattcaag tgcagcatca tttacttgaa gcttttcaag agcatctcta acattctcat
     2341 acttttctcc ttctcctgga tatataccac aatataccat tggagtagct tttttgtatc
     2401 ctggtaaagg ttcttctgta ggattatttg aatctgttat tgtatcacca acacgacaac
     2461 ttcttatatc ttttatacta gcagcaatat aaccaacatc acctgctgat aattcactaa
     2521 gttcagtttg acctggagcc ataacaccaa cttcagttac ctcaaacttt ttattggtat
     2581 tcatcatttt tattgtcata ccctttttaa cagttccttc aaatactcta acataagcta
     2641 caacaccttt gtaagcatca tagtaagaat cgaatattaa tgcctttaat ggagcttcat
     2701 tatctccctt aggtggagga acatttttta caatatcttc taaaacatct tctatgttta
     2761 aaccagtttt agctgaaata agaggagctt cacttgaatc aagccctatt aaatcttcta
     2821 tttcattttt tacttcctct ggtcttgcac ttggtaaatc tattttattt attattggta
     2881 atatttctaa atcttggtct atagcaagat aaacattggc taaagtttgt gcctcaacac
     2941 cttgagctgc atcaactact aatagtgctc cttcacatgc agctaaactt cttgatacct
     3001 catagttaaa gtctacatgt ccaggtgtat ctataagatt taaataatat tcatttccat
     3061 ccttcgcttt atacattaat cttatgtttt gaagctttat agtgattcct ctttctcttt
     3121 caaggtccat attgtcaagt aattgacttt tcatgtctct ttcactaaca agcccagttt
     3181 gttgtattaa tctatcagct aaggtagact ttccatggtc tatatgtgca attatactaa
     3241 aatttctagt tctactttgt ttattgtcca ccttaaaatt ccctcctctt gattattctt
     3301 atagttatta ttttacatta tctaaaaact ctttatatac caaatttaaa atttttgaat
     3361 ataaactaat tggcactaaa caaaattatc cacattatat gtggattatc aagtctaaca
     3421 ctaaatatta tataatatcg taaacttaaa gtaaatccta taaaatacag ttaatgaatt
     3481 tttattttat acattaaaaa tatataacta aaataaggct tattttaatc aataaacctt
     3541 attcacttat tactacttat taaatttttt tatgatttca tgtatatcaa aatttggtat
     3601 gtcaaaatca aattttgtta tatcaaattt aatacttgga aaatcatatt gtttcattcc
     3661 tagcatactt tttgcattac tgtctactat aaatacacaa actgatgtac ctataatcat
     3721 agtaatcaaa aataagattc tcataatata cttgtatttt cttagtttac ttttactttc
     3781 tatatttttt tctaatcggc tcatttagca tgctcctaat tataacttaa agctctaatt
     3841 tttttgttta aaatattcat ctaaaatctc ggctaaatac tttgtagtag ccttagattc
     3901 ttctacactt gttgcgttat tccctatttc tactaacata tgattcttaa ctgcaaactg
     3961 attgaatcta gcatagtctt tttctactat tggagatact agaccaggat actttttctc
     4021 agcaaaagca gttatatctt ctgctaaagc tctaagttgt tttctatttg tatttttacc
     4081 tcctacaaca aagaagaact ttgatactct ctctccattt atcatagttg tatattttaa
     4141 atggtctttt tccttagtgg ctggattagt taaatcccta gcatctctat gtaagtctat
     4201 cgctatatcc actgagttat atttaggtaa tatactttgt attgttttta agctacttgc
     4261 ataagagtta ttataagatg gatagtcatg gtatttagta gtatggacta caccccatcc
     4321 tttgctatct aaagcactag ttaatgcact tcctacttcc attactgaat ttttcttatc
     4381 tcttgaatga taatttccat caggtgaatt tgaataagtc tcacatccat gagtatgata
     4441 tatcaaaatt cttggatttt ctttggttac tcttaaatca tccttataat ctgaactaga
     4501 tgttgtgtta gtttcagttg tgtctgaatt ttttgactca atatctggaa cattttcttt
     4561 accaacataa agttttatat attctttttc actctctttt ttattatcaa ctttacttgc
     4621 atctttagat ttagtgtttt cttctttact ttcttcctta ttttctttgc ttgtttcttt
     4681 atttttatta ttctttttat tttctgtatc atttccttcg actttagctt ctggataaga
     4741 tgaatttact aaaaacttta aaaaatcatc ctgattcaaa gctaatgatt ttcctggtaa
     4801 aatgcatgct aatataaaag tcaaggttac tactttaatg cattttttaa acataattgc
     4861 tcctccttat tttataccaa tctacctgga tgtaaagacc tatttaagcc ttcacttatt
     4921 attatagata gattttcgat tgtatcatct atatcctttg gtgttactat taagttcttg
     4981 tcatatgggt ctaacgaatc ttttattaga tgatattttt cttcttcttt aagttttttc
     5041 aacatttcat aaaaactttc tgtttcttct gattgagcta ttaaattgtc tattgccata
     5101 tctaagacat ctattgtaag agttgctgca tcaaccaccg ttggaacgcc tattgcaata
     5161 acatccacgc ctaaagtttc tttagttaga gattttctag tattgccaac acctccacca
     5221 ggtgatattc ctgctgttga tatctgtata gtagagttta ctctttccat ttttcttgaa
     5281 gccaatgcgt ctattgctac tactctatct ggttttactt tttcaaccaa tgactttact
     5341 atttcactag tttctattcc agtaacaccc ataactcctg ggcttaaagc tgatacctca
     5401 gtgaaatcat catcatagtc tttattataa tttttaaaaa gatgtctagt tacaagtgtt
     5461 tttgatacac tctttggtcc taacgcatca gaagtaatat ttctatttcc aagaccaatt
     5521 accagggttt ttttattttt gtcttggcca aatatatcta ctaactcctc ttttaaataa
     5581 cttatcattt cttctctcga ctcatcatca tcaaacttca taagattact ttccagagtt
     5641 atatactttc ctatatcttt attcattatc tctgcgcctt gcctgtctat tacttcaact
     5701 ttagttacaa tgcaattttc taattcttta gtttctattt ttacaccagg aatactactt
     5761 ccttcttgag acttttcaca catttcacta gcttctaaag ctaaatctgt tcttacatta
     5821 atcatataaa cctccagtat aaattaaatg ctaattataa ttgttaacaa aatttagtat
     5881 tttataacaa gaaattgtaa atttatttaa aacaagtaaa attaaggctt tcaatgtaag
     5941 aaaattgggt ttagtcagtg atttgtcagt aaactttctt atctatcatt tatttacaag
     6001 caaaaaaagg tagcaactat ttatataggc gctaccttta atctatttat aattttccta
     6061 ttaagctttc actaatatat taactaaaat aactttttta tatttatacc taatttattt
     6121 actatataaa aaaacagcaa taattataca ataatcacct aacaccttta ttccttttgg
     6181 aaaaatggtt ctgcaatttc caaccattca tttggaagat aaagcgataa atacccatga
     6241 ttatagccct tagcttcata aatctcacaa gagggtacat aagactgaaa catttttgca
     6301 gattttttaa cgcacttcat ttctttttct ccataccagt acattacttg tgctgtggtt
     6361 tttttaacat tctctttaag tgtatagttc atcatataag tacgatacat attatagaat
     6421 gtttcttttc ttacatgtgg catatctgtc atataatacg cttttatttc atctggatat
     6481 tgcatatttt caggtaacaa ttttggcatc atagcaattt gaaagcgaca agccttctca
     6541 ctaaacaaaa gtcctccaaa aaatcgaata ccagcaatac aaaaccttgc cataagaggt
     6601 cttgggtagc aaatactacc atcaataatt gcttttttag taatattagg ttttcgagat
     6661 aaaagttcca tgacaatttg accaccaagt gataccccac acaaagcgaa aagatgtcca
     6721 ctgcaatttt catctatata tgatatcaac ttatctgctg tatcctctgt agaaatgtat
     6781 gttgtttcat attcttctcc atgaccatcc aatgtaggaa gtatcacatg atagttgtta
     6841 gacaaaactt ctgcttgtcg caaatagttc caccacgcat tccctccccc atgaatcaac
     6901 ataatatgtg gtttgttttt atctccaaat tcatgaaatt tcatatacaa cctcctttta
     6961 taaaaataag attcatgtta ttttgtttta tcattatcaa ccacaggtgg agaagatata
     7021 attgaaagtt ttctaagttt tagatttgat ttaggctttt gttctatata tggtttcaat
     7081 aaatcagcta tacttttaag aagattttca tattcattat cactcatata tagtaatgct
     7141 gaagagaaaa atacattatc ctttttatgg tcttgctctt ttctgctaaa gtattcattc
     7201 atttcctgca tcagacctac ataaaatgct gtagaaagtg caacgaaggt ctctccttcc
     7261 atagatgttg tatattcctt tagtgcataa accttttcaa tagtaccacg cacacgattt
     7321 tctttaatta cttcaatcat attgttctct tctaaaattt ttacatggtg ataaactgtt
     7381 gcacgaggaa tatcatttaa atactttaca atatcagatg tgcgagcact gccatgaagt
     7441 cgaatatatt gtaagatacg aagccttgca ggatttaaaa ttagttttgc tatgtccatc
     7501 ttatcagccc tttctttagt ctataaaaat attatagtct atttttttag actattcaag
     7561 tctatgatat aagtagtaat tacaactata ataaatgcta caaatgctat tctatataaa
     7621 cactgagatt aattacatat aaccttctaa catattagtt atattgtttg atgcatctat
     7681 catacatact a
//
LOCUS       DAMJVX010000163         7641 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMJVX010000163 DAMJVX010000000
VERSION     DAMJVX010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.169"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            61..1119
                     /locus_tag="PC407_003036"
     CDS             61..1119
                     /locus_tag="PC407_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HDF2498447.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTI
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCIFLENCDFNRTIFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            1223..2407
                     /locus_tag="PC407_003037"
     CDS             1223..2407
                     /locus_tag="PC407_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2498448.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADGKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNEKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGVIKYYTSSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2614..3327
                     /gene="rpsB"
                     /locus_tag="PC407_003038"
     CDS             2614..3327
                     /gene="rpsB"
                     /locus_tag="PC407_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDF2498449.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3406..4317
                     /locus_tag="PC407_003039"
     CDS             3406..4317
                     /locus_tag="PC407_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDF2498450.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4402..5106
                     /locus_tag="PC407_003040"
     CDS             4402..5106
                     /locus_tag="PC407_003040"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDF2498451.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            5132..5689
                     /gene="frr"
                     /locus_tag="PC407_003041"
     CDS             5132..5689
                     /gene="frr"
                     /locus_tag="PC407_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDF2498452.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            5692..5895
                     /locus_tag="PC407_003042"
     CDS             5692..5895
                     /locus_tag="PC407_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498453.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5924..6658
                     /locus_tag="PC407_003043"
     CDS             5924..6658
                     /locus_tag="PC407_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDF2498454.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6671..7450
                     /locus_tag="PC407_003044"
     CDS             6671..7450
                     /locus_tag="PC407_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDF2498455.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         3147 a          786 c         1460 g         2248 t
ORIGIN      
        1 aataatatcc aaactaatac aaaatagtaa taattgtttt gtactggagg atatatattt
       61 atgaattgta aatggatatc taagatagat gagagaaaaa aatgtcatag agaagcagat
      121 tcttctggat attgtatctt tcataaagaa aataagtctg atgaagagat acagttgatg
      181 atggatacta ttcataaaga agagataagt gaatttaatg gatttgtttt tgaaaatgaa
      241 tttaatgcgg aagaaatatt aacttacaat tataaaatac tggatttttc tgaatccata
      301 tttaaacaaa aagcaaattt taaaaaatat atttttaaaa agaatattat ttttaactat
      361 acagaattta gagataaagt tttatttaat ggttgtatat tcttagaaaa ctgtgatttt
      421 aatagaacca tatttagtaa aaactatata aatgaccgta tatttgaaaa agtgaaattt
      481 aaaggacctg atttggtagt taataaagta gaaaattttc cacgaatgga tggaataatt
      541 tttagcatgt gtacaaaatt tgtgttaaag aatgttgaat atggaaaaag tgagtatgag
      601 catggcaaga taaattatag aatagctaga aatcaagcaa caaaaattgg agaatatgag
      661 atgataggat tttactatta taaggaaaga atttatagta gtaaaataat gaaacgttct
      721 aattatccta ctttcagtga ttatttagtt gaaaaatttt ttgaccaaat agcacgttac
      781 acaacaggtt atggagaaaa gccatggaat atattattag tgattatagc aataataagt
      841 gtttttgctt tattatactt atttgtaggc atagaaagtt caaatagcac acttgtagct
      901 ttagatataa acaatatagg tgattactca ctaagtgaga ttttcaaaat gtatatggat
      961 ttatggtatt ttagcatggc tacatttagt acagtaggat atggagatat ggttgctaca
     1021 agtttgattg ggaaagcatt ggcaggtata gaagtatttt ttggagttac aataggagca
     1081 atttgggcat ctgtgattat taaaagaatg ataaggtaat atttgtgtaa atatggatag
     1141 gtattaatta ctagatataa attggaccaa atagggaaaa tttaaatata aatatactta
     1201 ataatccaag aaggtgaaaa atttgaaaaa tgcactaaaa aaaattatat cattagccat
     1261 gattctagtt tttataatac caaattattc atatgcagat ggtaaatctg tagacaagta
     1321 ttcaaaaaca tctatactta tagaccaaga aacaggtaga gttttatata gtaaagaacc
     1381 tgatatgaag gtaccattag ctagtttaag taagatgatg acttttttac ttgcaataga
     1441 agctattgaa aataaagaag taaaagaaac tgataaggta aaaatagaca aatctattgc
     1501 atcagtaaaa gggtcaagtt ataaacttaa agatggtgaa gaagtgccac ttatagagct
     1561 tatgagaggt cttatgattg tatcaggaaa tgatgcggca attgctatag caaaacatat
     1621 ttgtaaaacg aaagaagcat ttgtagatag aatgaataaa aaagcaaaag aaataggaat
     1681 gagtaataca cattttctaa atccaaatgg tcttccaata tacgatttaa gcaaccccaa
     1741 aaaaccagca aaagaaaata tctctactgc aaaagatata gctattcttg gaaaatacat
     1801 gtttgaccat tatgaaaaac aagtaacagc tataactgat atggagacat atacaaattc
     1861 agatagaagt tttgaaaaaa gcaatacaaa tgcattatta agaattatac cagaagtaga
     1921 tggtataaaa actggatata ctggcaatgc tggatattgc ttatcatttt ctatggttgt
     1981 aaataaaaat gagaaaaatg agaaaaaacg tagagtaata ggggttgtat taggtactaa
     2041 ccataaaaat aaaagaacct ctgcttcatt agctctactg aaatatggaa aagaaaattt
     2101 caatatgaaa aaagtaattg ataaagatag ctttattggt aagaaatata taaaaggaat
     2161 gaaagagtta gaggttacat taaaagcaaa agatgaactt tatacaatat taaaagatga
     2221 tgaaagtata aaatcagaag ttaagattaa aaatatagag taccctgtta aaaaaggtga
     2281 tactatgggt gttataaaat attatactag ttcaggtgac ttacttggaa gtgtagatat
     2341 agtaagtaat aatagtatag ataacgtatc attaaaaaca aaattaaaaa tgaaatttgc
     2401 aaattaaatt caaaaaatgg tataaaatac acaaaaaaat ataaaaatag aaaatatgga
     2461 aaaaatattg ttatttcttc gcctctgtga tatactacta gaggtgaaaa aataaaataa
     2521 atcacacaca gcattgggat ccttaaaagg tgcctattac atggtttttt agggagaaga
     2581 tcaagctgga ggtataaaac caggaggtaa gatatgtcag taatatcaat gaaacaatta
     2641 ttagaagcag gtgttcactt tggtcaccaa acaagaagat ggaaccctaa aatggctaag
     2701 tatatattca cagagagaaa tggaatatat ataatcgact tacaaaaaac agttaaaaaa
     2761 gttgaagaag cttacaagtt cactaaagaa gtagctgaaa caggaaagcc aatcttattc
     2821 gtaggaacta aaaaacaagc tcaagatgcg ataaaagatg aagctgaaag atgtggaatg
     2881 tactttgtta atgaaagatg gttaggtgga atgttaacaa accacaaaac tataaaaaca
     2941 agaataaata aattaagaga attagaaaaa atggaagaag aaggcgtatt taatgttctt
     3001 cctaaaaaag aagttataaa attaagagct gaaaaagaaa aactagaaaa atacttaggt
     3061 ggaataaaag acatgcctga attaccaggt gcaatgttcg tagttgaccc aagaaaagaa
     3121 aacatagcta ttcaagaagc tcatagatta ggtataccag tagttggtat agttgatact
     3181 aactgtgacc cagaacaatt agactttgct ataccaggaa atgatgacgc tataagagcc
     3241 gtaaaattaa taactggtgc tatggcaaca gctgtaattg aaggtagaca aggtgctgag
     3301 gaagaagtag ctgaagacca agaataataa tttgacaatt aaatggtaag ggtcgccctt
     3361 accatttata atatggaata gaataagtag gaggaaactt aaaaaatggc taatataact
     3421 gctcaaatgg taaaagagtt aagagaaagt acaggcgcag gaatgatgga ctgcaagaaa
     3481 gctttacaag aagctgaagg aaacatggaa aaagcagtag atttattaag agaaaaagga
     3541 ttatcaaaag ctgctaaaaa agctggtaga gttgctgctg aaggtctagt tgcaatagaa
     3601 atgaatgatg ataatacagt tgcttctatg gtagaagtta actcagagac agatttcgtt
     3661 gctaaaaacg aagacttcaa agtgttcgtt aaagatgctg cttgtatggc attagctact
     3721 gataaagaag atatagcttc tttattaggt gaaactcata aagagggaat aactttacaa
     3781 gaagttttaa ataatagagt tgcaaaaata ggtgagaaat tagactttag aagatttgct
     3841 aaagttgtaa ctaatggaca agttgctgga tacattcacg gtggtggaaa aataggtgtt
     3901 cttgttgaaa tggaaacaga agctagagat gctaaagttt tagaattagg taaagatgta
     3961 gctatgcaag ttgctgctat gaatcctaag tatgtttcaa gagatgaagt agatgctgaa
     4021 tacatagcac atgaaacaga agtattaact caacaagctt taaatgaagg aaaaccagct
     4081 aatatagttg aaaaaatggt taaaggaaga ttagaaaaag aattaaaaga ggtttgttta
     4141 ttagagcaaa ctttcgttaa aaatccagat ataactgtta aacaattagt tgctgatgtt
     4201 gctaaggcag taggttctga tataaaagtt gttaaagttg tgagattcga agttggtgaa
     4261 ggtatacaaa agagagaaga aaactttgca gaagaagttg ctaagcaact taagtaataa
     4321 ccaaagagag aacacgcagg tgttctcttt tttaaaaaat agaaaaaatt ggatatgaag
     4381 gattgaaata ggaggtttct gatggataaa ccaatgtaca aaagggtatt gttaaaatta
     4441 agtggagaag cattagcagg agaaagaggt tttggtataa ataatgatgt agttaatgat
     4501 attgctattg ctataaaaaa aatacaagaa ataggtgttg aagtagctgt agtagtaggt
     4561 ggaggaaact tctggagagg cagaaccagt gaaggaatgg atagaactac tgctgactat
     4621 ataggaatgt tagctacagt tatgaatgca atggcgctac aggatgcact agaaaatata
     4681 gatgttgcta caagagttca aactgctata gacatgagac aaattgcaga accatatata
     4741 agaagaagag cagttagaca cttagaaaag gaaagagttg ttatatttgg tgcaggaaca
     4801 ggaaaccctt actttacaac agatacaact gctgctttac gtgctgctga gatggaagca
     4861 gaagttatat tgctagctaa aaatgttgat gcagtatatg acaaggatcc aaaagtacat
     4921 gcagatgcta agaaatttac agaattaagt tacatggaag ttatacagaa agaattaaaa
     4981 gtaatggatt ctacagctac atctttatgt atggataata aaattccaat aaaggtattt
     5041 gagcttacaa cagagaatat aataagagca gtaaaaggcg aaaatatagg aactactgta
     5101 aaataatgaa gaatgaagga ggagtaaaat tatgaaactg gaaatacata agcaattaga
     5161 agagaaaatg aatgggacaa ttgatgcttt aaagtttgaa tttggaacta taagagcagg
     5221 aagagctaat gcacaaatgc tagacaaaat aagagttgat tattatggaa ctccaactcc
     5281 aataaatcaa ataggtgcta tatctgtacc agaaccaaga atattaatga tatctccttg
     5341 ggataaatct gctatgcatg aaatagaaaa agcgatagct aactcagact taggattaaa
     5401 tccatcaaac gatggtgagg ttataagact ttctgtacct gctttaacag aagaaagaag
     5461 aaaagaatta gctaaaaaag ctagtaaagc tgcagaagaa tttaaagtta gaataagaaa
     5521 tgaaagaaga gacgccaatg agaaaattaa aaagatggaa aaaggcggag aattaactga
     5581 agatgaatta aaaaaagcac aagatgaagt tcaaaaaatg actgataaat ttataaaaga
     5641 aatagataca ttattatcta aaaaagaaaa ggacataatg gaggtataat attgaaaaaa
     5701 tattataacc ttttaggtct tcatgtagat gaggtaaaac aatattttga tgagcaaaat
     5761 ataaattata ccgtaaaatc tattgaaggt aaaaaggata aagaaaaact tgtagttcct
     5821 agagttataa aaatatctga gataggtgat agtgtagaac tgataatcac atacttttct
     5881 gactccttag tttaaggagt cagaacaata ttggaggcaa aatatgaata acaacattat
     5941 atatgatata gacttaaata acatacctac tcatattgct attataatgg atggaaatgg
     6001 aagatgggca aaagctagat ttcttccaag aactgcagga cataaggctg gagtagaaac
     6061 tataagagat atagtaaaag aatgctcaaa attaggtgtt aagcacttaa cattatatgc
     6121 attttcaaca gaaaactgga aaagaccgaa acttgaagtt gacacactta tgaacttatt
     6181 atctacatac ttaagaaatg aaatagctga acttcaccaa aacaatgtaa aagtaactgc
     6241 tataggagat ataagtgctt taccaaaaac ttgtataaga gaattaaact cagctaagga
     6301 actgacaaaa aacaatacag gagttaactt aaatttagca ttaaactacg gaagtagagc
     6361 agatataaaa aatgctctta tagatatagt aaaaaattgt gaaagtggta aaatagatat
     6421 aaacaatatt gatgaagata ttataaaaaa ctatttaagc acaaagtcta taccagatcc
     6481 agacttggta ataagaacaa gtggtgaaca aagattgagt aactttttac tttgggaagt
     6541 tgcttattct gagttttact ttactgatat acattggcct gattttaaaa aagaagaact
     6601 acagaaggct atatacgtat atcaaagaag ggatagaaga tttggaggac ttaagtaagg
     6661 agattatttt atgcttacaa gaattattgc ttcactagct ctggttccat tatttctatt
     6721 tgttgtatat ggaggtattc ctctatatat agctgagaca gcaatagttt atattgcatt
     6781 gcatgagttt tataaggcat ttaaaataaa agatgtacat ccaatattca ttataggata
     6841 tttattttct atttatttag ctgtaaaaaa tatttttaat ttaccattag aatatactta
     6901 tgcagtaatt tttattttat ttttagcaag tataatttat atgttgatgg gcaaaaacaa
     6961 tgttatagat gtttcgatta cgtttttagg agttttttat ataggagtat ttttagattt
     7021 tataattata actataaatg gatttgaaaa aggtagtata tatgtatggc ttatatttgt
     7081 gatatctttt atgacagata tatttgcata ttttagtggt tatttacttg gaaaacataa
     7141 gctaattcct aaagttagtc ctaaaaagac tatagaagga gctattggag gaataatagg
     7201 aagtacactt tgttgtattt tatttggata tttatttggc attgatttgc ttcaattagc
     7261 gataatagga agtataggaa gtgtaatagc tcaattagga gatttatttg catcttctat
     7321 aaagagatat gtagggataa aggattatgg aaagataata cctggtcatg ggggtatatt
     7381 agatagattt gatagtgtta tattagttgc accttttgta tatagtgcga ttaaattttt
     7441 tattagataa agattggttg gatttaaaag agcaatctat aattgctagt aatgaaaata
     7501 gctatgtaac ttttaaatgc agagatattc tgcagttatt agttacatgg ctattttcat
     7561 tgttcaaaaa tttagaagac cttggggaat tttataagaa taagtattac tttataatag
     7621 tatactatat ttgtagtgag t
//
LOCUS       DAMJVX010000164         7623 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMJVX010000164 DAMJVX010000000
VERSION     DAMJVX010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.170"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            87..2315
                     /locus_tag="PC407_003045"
     CDS             87..2315
                     /locus_tag="PC407_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDF2498456.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            complement(2553..2852)
                     /locus_tag="PC407_003046"
     CDS             complement(2553..2852)
                     /locus_tag="PC407_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDF2498457.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QDLINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            3059..6100
                     /locus_tag="PC407_003047"
     CDS             3059..6100
                     /locus_tag="PC407_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HDF2498458.1"
                     /translation="MFKQRISKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGNTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            complement(6261..7535)
                     /locus_tag="PC407_003048"
     CDS             complement(6261..7535)
                     /locus_tag="PC407_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2498459.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
BASE COUNT         2813 a         1069 c         1099 g         2642 t
ORIGIN      
        1 tttaacttta ctatttgtgt aaaaagtaat ataatttaaa attgattata aatctaactt
       61 ttttctttat caaaatattt taggaggtga gacatttgaa aaaaactaat agtttaaaag
      121 aaattttaaa ctggaataat atcaagaatt taagaccttc aaagggttta aaaaatatat
      181 tagaaatttt taaaagtgac ctaaaagata tacgtggcaa ttatgccgta cttgtaatag
      241 ttttaggtct tgctttttta ccatctctgt atgcttggtt caatataaag gcatcttggg
      301 acccttatgg aagcactgct aatatgcttg tagcagtaac aaatgaagat aaaggaaatg
      361 aaatagcagg acaaaagatt aatgtaggag accaattaat tgaacaactc aaagataaca
      421 agagtttagg ttggcagttt gtagatgaaa aaactgctct tgaaggtgtt aaaaaaggta
      481 cttattatgc atcacttaag attccaaaaa attttacaaa agatttgaca tctttaatta
      541 cagatgatgt taaaaaaggc gaaattatct atactgttaa tgaaaaaata aatgcagttg
      601 caccaaagat tacagataaa ggtgcttcta caatacaact acaggttaat cagactatag
      661 taaaaacagt tagtgaaatc gttcttggta tatcaaacgg agttggtgca ggaattgaac
      721 acagtcttcc aaaattgaca aaagttgaaa attcccttat tgaccttcaa aagagatttg
      781 accatataaa taagactgtg gatttagctt ctgatgctac ttcaaaatta tctgatacag
      841 ttaaagatat taaatctgat ttacctacaa ttaaaaagac tctgaatgat acaaaactat
      901 tatcaagtga cttaaaaaaa ttcttagagg atacaaatga taatttagac gaactttctc
      961 ctcttattaa gtctgactta aatttaatgg ttgacctttc atcttctgct tcttctctta
     1021 cattaaattt aattgatgca gtaaatagtg gcagtgaaga tgtacctaag ttgatagata
     1081 atttatcaga aaaactttct aatttgcaat ctttaaacga tactttggtg gactttttaa
     1141 ctaaattaaa ccaacttaca tcaaataata gattggatga tgtaatagat aatcttgaag
     1201 actctagcaa taaaatagat tcatctatat caacattaaa tgatattaaa aacaaagtta
     1261 tatctggaca acaaccatct ataagtgctt tgaataatgt tttaagtcta tcaaatggta
     1321 ttggaagaat taatttaaat attttaaata attttgactc aaaaatatca aaacctataa
     1381 atagtatatt tgcaaacagt ataaaagtag caaatgatat aattactgtt ttagataagg
     1441 cagaagcaaa acttccaaag gttgaagaaa tacttactac ttctctaaaa ctttcaggaa
     1501 atgcacagga aagtatatca ttgataagag aaagacttcc tttagcaaaa ggaatgttgg
     1561 atgatttgat tgatacttta agcaaaatta gtaatggtga agatatgaaa aaattagtca
     1621 gcttgcttga aagtgatata ttgacaaagt ctaatttctt aaaagaacct gttgaggtga
     1681 aaacagagaa attatatcca attgaaaatt atggttctgc aatgacacca ttttatagtg
     1741 ttctttctac atgggttggt atattactta tttcagcact attgtctaca tctgtacatg
     1801 gaaactataa accttatgaa atttactttg gaagaggtct tactttttta agtatagctc
     1861 ttgtccaagg ttttataata ggaactggag atatattatt gttaggagta acagcaaaac
     1921 atccaatttt attgatagtt ctgcttatgt ttactagtat ggtatttaac tttatagtgt
     1981 attctttagt ttctgtattt ggaaatgtag gaaaagcaat tgcaatagta ctattagtcg
     2041 ttcagattgg ttcttctggt ggtacattcc ctatacaagt tacacctaca ttcttccaaa
     2101 gaataaaccc tataatgcca tttacctatg caataggtgc tacaagggaa gcagttggtg
     2161 gtatatatgc tccaaatcta actaaagata tactagttct attactattt atgattctgt
     2221 ctatttgttt aaacgtactt ctaaaaggac ctataaatag agttataaaa ccatttaatg
     2281 ataaatttgg cgatagtaat ttaactggtc attaaaaaat taattctgtc tttaaatata
     2341 tttttttaat aatattctaa gttcatatta aaaggtagta gctaaaaagc tactaccttt
     2401 ttccaactta aaaactaata tttactacaa aaactgtcat agaaaaaagc gaaaaaattc
     2461 gttctttcct aaatttatct tataaatatt tagatatatt cctacttaat aaacagagct
     2521 atacatagta tttaatgtat aacttacctt atttaactca tataatcaat tattcctgaa
     2581 tttaaattat ataaatgctc aaaaccttct tcagctaaca aattacatgc agtaacactt
     2641 ctatgaccac ttctgcaata aactactact ttcttatctt tataatcgta aatttcatct
     2701 atattattga ttaaatcttg taaagatata tttatagcat tttctatttt atattcctta
     2761 aattcatctt tagttcttac atctaataat aaaacatcct tttcattttt catcatttct
     2821 ttaagttgct tcttatttat attcttatac atatgtctaa aattcctcca ttttatttat
     2881 atattaccta taccctttat tctttataca acaacaaata ttatttttat atttttctct
     2941 gttatataaa aagctatact tataaaagct ttttttattg ataataatct gttgattaaa
     3001 ataattaaga atgttatgct aattataaat tattaaaaag ggaaagaggt aatgtaatat
     3061 gtttaagcaa aggatctcta aattattatc tagtacatta gtattgagta tgttatttac
     3121 tgctgctcca aatattactt ttgctgataa tacaaaagat aattccgaaa aatatcaaag
     3181 ttctgatata gaattacatg attatagtaa aaacgcggaa tcttacacaa aaactaaagc
     3241 tcttgctaaa gagaaaatcc aaactttgct atcaaagtat ggtgctgtaa gtgctcaata
     3301 tgctttgatt gataatggca aaattgagat ttctggtaat ggtggagttt acagcaaaca
     3361 ggataacaaa aacttaaata aggataatat gtatagtatc gcatctatca gtaaaatgtt
     3421 tactacaaca gctgttatga agttggttga tgatggaaaa ttaaatttag atactcctgt
     3481 tgtaaagtat atacctgaat ttaaaatggc tgatgataga tacaaggaaa tcactccaag
     3541 aatgttatta aatcattcat cagggttgat gggaagtagt tttaaaaaca ctattttatt
     3601 agcagacaat gattcttatg gtcatgataa tttcttaaaa gaattacaaa aacaaagatt
     3661 aaaagctaaa ccaggagctt tttccgttta ttgtaatgat ggatttactt tagctgaaat
     3721 tttagtagaa agagtatctg gtatgtcatt tactaatttt cttgataaat acataaataa
     3781 tccacttaac ttacaaaata caaaaactac agaaaatagt tttgatagtt caaaactagc
     3841 aaaagcatat gtaccttatt gggaagatgc agttccacaa gataacctta atgctatagg
     3901 tgcaggtggt ttatattcaa gtgcagaaaa tctatgtaca tttgcacaaa catttatgaa
     3961 aaattcaaat ggtattcttt ctcctgcttc agtaaaagct atggaaaaca aagaatattt
     4021 aaatggtcta tggcctgaag gagaagattc tatacttgga tatggtttag ggtgggattg
     4081 tgtcaacact tatccattta atcagtataa tttaaaagct cttacaaaag gtggagactc
     4141 tttattattc cacagtaatc ttatagttct tccagatgaa aatatggcag ttgcagttct
     4201 ttcttctggt ggaagtagtc aacttaacga aataattgga caagaaatct tactatctgc
     4261 attaaaagaa aaaggtaaaa ttaaagaaat aaaacctgat aaaacattta gtaagccaca
     4321 acaagttaaa atgcctagct ctctaaaaga aaatagtggt ctttatgctt cttcaaatat
     4381 gataaaagta gatgttaatg ataatggtac tttaacagta tcttctcctt atatagaaaa
     4441 tggacctgaa gataaatacg tatacatagg tcaagataga tttgtatctg aaaagggtaa
     4501 cagttgttta aaatttgtaa aagaaaaaaa taatatcaca tatttaaata tgagctctta
     4561 tgatgatgtc cctggattag gtcagacagc aagtttatac tatgtagctc aaaaagtaga
     4621 tgacaacaat atatcaaata gtgtcaaaga agtgtggaaa aaaagaagtg gcaaaggtta
     4681 ctatctagtg gatgaaaaat atacttctca aagctatatg tttggctctg taaaagcaag
     4741 ttttagtctt tctgatgaaa ctccaggtta tatagttaac actaaaataa tggatgagaa
     4801 taactcaaat gcttttatag aaatacctgg tgtaataggt cgtgatttat ccgacattaa
     4861 acttcataaa gaaaatggta cagagtattt atcttttgga acactaacgt atgttagtga
     4921 agattctata actaatttgc cagcagaaaa atcttttact tgtgaactag aatccaatgg
     4981 ttatgcaaaa tggtataaga ttggtgacga tatcgcaaat aaaaaaatag aagttaatct
     5041 tcctcaaaat tcagcttttg ctgtttatga tgataaaggt gttcctgtaa actattcttt
     5101 agttacaaag aacaatagag ttagattgcc aaaaggaggc gtaattgttt tcttaggaag
     5161 tccaaatgct agatttgagg ttacatatca agatgaagta aatgcaagtg cacttacagg
     5221 aactgataga tatgaaactt ctattaagat aagtcaagca ggttgggaaa atgctgaaaa
     5281 tgcagtttta ataaacgatt cagcaattgc agatgctttg gccgcaacac catttgcata
     5341 caagaaaaac gctcctatat tattgactgg aagctctcaa ataaatgaaa aaactttggc
     5401 tgaattaaag agattaaaag ttaaaaatgt atatgtagtt ggtggagagg cttctataaa
     5461 tgaaaaatct ttagatacta taaaatcaaa caatatctct gtatctagaa tttcaggaag
     5521 tgatagatat caaacttcta tgaacattgc taaagaattg aacaatatta gcaatatatc
     5581 aaagatttca gtagttaatg gcgaaaaagg attagccgat gcagtaagca ttggagctgt
     5641 atcagctcaa aatgatatgc ctataatact tactaatgaa aatagtaata taactgaaat
     5701 aaataactta tttaaaaaca aaaagattga taaatcttat gtaatcggtg gagaatatac
     5761 agtttctaaa aatatagaaa gtaaacttca aaatccacag agaatatcag gtaacactag
     5821 aaatgaaact aatgcaaaag taataaaaga attttacaag gattctaaga tagacaattt
     5881 atatgttgct aaaaatggaa tgaataaaca agatgattta atagacggat tatctgttgg
     5941 agttttggct ggaaaaacta aatctcctgt aatgctagta ggaaactcac tagattataa
     6001 tcaaaaagaa ctatttaaaa ctatgagatt taaatctgta acacaaattg gtggaaatgg
     6061 aaatgaaaac tcatttaagc aaataaaaga aatagcttaa cataactaaa aaaggctgta
     6121 ttcaagtaat attaattgaa tatagccttt ttttatttaa gttataagtt ttatatgtgt
     6181 aaaaattaaa tttaaatatt aaatgataag tttattaaga tatagataag tctaaaactt
     6241 tgcctttttt aagttagttt ctatttatca ttcatactta acaatttatc taaatatact
     6301 gactttaatt gtttactggc taaaatcttt ttagctggtt caagctttaa tatagctgac
     6361 agtatagctc ccatagctct atgactagcc agagcattat tatcaaaaat taaattttgt
     6421 aactgccctt tttcaattgc catgagaact gctctatgta ctgaatttgc tggagttatt
     6481 attttttcat ctctctttaa taacataatg ctatttttat ggcaatttct aacacaaact
     6541 ccacaaccta aacatctatc ctcatcaatt tttatatatt ctttaccatc ttccttaacc
     6601 ttgcttatcg catctattgg acatgcagtt atacatttac cacactttac acaattttca
     6661 tggtttatat ttggtataaa acttgttgtc tgtactggat gaagatttcc aaatctttta
     6721 gctgctacca ttgcttcaca gcaacatcca cagcaattac atataaacgt aacccctttc
     6781 ctaacatttt ctccacattg aactaagtta tgttcatatg cttgatgaag tagttcttta
     6841 cattctattt tatccacacg tcttgcaaat ttattattta ttaaagaatt tgctacattg
     6901 tcaaacgtca tacatatatc catgggagca tcacaagctt ttccaacatg ttgcattcta
     6961 tgtctacagt aacacatact tatacctata tgagttgatt catcaattat atgagtagcc
     7021 ctttcataat ctaaaatact aacctcatta tcattagtta aaacttcctc ttgaacgtat
     7081 actcttccaa gcttcgtttc tgttgaataa aataaatctt tgataaaatc ttcttctaca
     7141 ttcatatatt gatagtaaag ttctgccaaa agtttttggt ctatatcgtg tctcgttctc
     7201 atcatggcaa actcaaaaaa accagccatt ggaggaggca ttatatattt cttcccttta
     7261 ttatcttcta tatctagtat cagagcctta ctagcaagtt tatccaatac tctatatgct
     7321 tcactttcac ttacactcca aattttagct gctgttttta ctctgaaagg ctttattgga
     7381 agctgagcaa ctaacttcgc ctcttgttct gtatatagta catttagtat tttgtataat
     7441 gtatcagatg gtggagcacc ttgtggaaac ttgtttattc tttcctctaa acttttatac
     7501 atattcttcg cagttatatg agaaatagta atcaccttct ttttcttttg ttttcaaata
     7561 aaatcaaata ttctttctta ttatatattc tcttacttta tataatacca attctttcta
     7621 aat
//
LOCUS       DAMJVX010000165         7535 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMJVX010000165 DAMJVX010000000
VERSION     DAMJVX010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.171"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            108..1433
                     /gene="cdeA"
                     /locus_tag="PC407_003049"
     CDS             108..1433
                     /gene="cdeA"
                     /locus_tag="PC407_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HDF2498460.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GISFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFNYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKGRYKY"
     gene            complement(1528..2322)
                     /locus_tag="PC407_003050"
     CDS             complement(1528..2322)
                     /locus_tag="PC407_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2498461.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2323..3198)
                     /locus_tag="PC407_003051"
     CDS             complement(2323..3198)
                     /locus_tag="PC407_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498462.1"
                     /translation="MRSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3202..3570)
                     /locus_tag="PC407_003052"
     CDS             complement(3202..3570)
                     /locus_tag="PC407_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2498463.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3753..4412)
                     /gene="deoC"
                     /locus_tag="PC407_003053"
     CDS             complement(3753..4412)
                     /gene="deoC"
                     /locus_tag="PC407_003053"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDF2498464.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4448..5992)
                     /gene="murJ"
                     /locus_tag="PC407_003054"
     CDS             complement(4448..5992)
                     /gene="murJ"
                     /locus_tag="PC407_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2498465.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILITGIIFIGITSVMSAFLQIKENFIVVGF
                     GSIPYNIVIIISIMLSTVFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKD
                     DSLIKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYSDRLNGFVTGTFTA
                     SIVSVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFER
                     GAFDARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVL
                     DLVLIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIM
                     GVLSYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKR
                     KLHLKK"
     gene            complement(6466..7350)
                     /locus_tag="PC407_003055"
     CDS             complement(6466..7350)
                     /locus_tag="PC407_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2498466.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
BASE COUNT         2506 a         1227 c          792 g         3010 t
ORIGIN      
        1 attttttgtt gattttaagc tgtttatcgt ataaataaaa tttttcttga catatagtta
       61 ctatatagct tattatagat agagagtaat aaaaggaggt aaaatttatg gaaaatttat
      121 ttacaagaaa attcactact tttgaatttc taaaatttgt ttctcctgca attatatcca
      181 tgatatttat atctttgtac acaataatag atggcatctt tgtatcaaca ttagttggtt
      241 ccgatgctct tgctagtata aatattgtac tacctataat taaccttgtt tgtggatttg
      301 gaataatgat ggcaacgggt ggaggtgcta tcgtttctat acgtatgggt gaaaatagac
      361 aggatgaagc caactctaca ttttctttta tagttttgtt ttcattgatt gttgggattt
      421 cattcacagt aatctcatat ttctttatca aagaaatatc tattttgctt ggtgcaacag
      481 ataagttatt accatattgt ataacttatg gtaaagttat gattttatgt acaccatttt
      541 atattttaaa atttatattt aactactttg caagaactga tggaaattct aaatttagtt
      601 tatttctatc agtcattggt ggtgtaacaa atataatatt ggattatgta tttattaaat
      661 attttggaat gggtctttta ggagctgcag ttgcaactgc tataggtatt attttaactt
      721 gtgttttagg tattatttac ttcttatcta ataaatctac actcaaatta agaaaaccaa
      781 aaaccgattt tagacttata agagatacta tgataaacgg ttcttctgaa atggttacag
      841 aattatctac aggaattaca acattcttat ttaatgtagt agctttaaaa ttagcaggag
      901 aaaatggact tgctgctctt actatagtat tgtatgctca ttttttaatg acatcagtct
      961 atctaggatt cgctgctgga gtgtctccat taataagcta taattttggt gctgaaaaca
     1021 gtgataaatt aaaagaaaca tttaaacatt ctctaaaatt tatatttgtt tcttctcttt
     1081 tagtgtttat tattgcttta gtatttgcac catttattgt tagggttttt gtaagtccag
     1141 ataacacagt atttaaacta gccttacaag gattaaaaat atttgcattt gcttttttgt
     1201 ttgttggtat aaatatattt gcatcaggat tttttacagc atttcacaat ggaaaaattt
     1261 cagctattat atcttttagt cgtgcctttg tttttataat cataggaatc ataattcttc
     1321 ctcctatgtt aaacatgact ggattatggc ttacagttcc atttgctgag gttataacca
     1381 tatttatatc tattctattt ataaaaaaat ataaaggtag atataagtat taaaaattaa
     1441 tgcttaaaat caacatttat taaaactaat aaacctgctt taaaatacaa atttacaaat
     1501 ttgattttta agcaggtttt attttagtta taataagtca tttgcatact ctttaattgg
     1561 tgcttttcta agtaataaaa ttataaatat tatgcaaata actataattc ctacctttgt
     1621 tccaaataaa ataaaaggat tctgaaatga ataaataatt ttttctaaat acaatagcat
     1681 tttatgcata tttttagttg caaaattaac tatataaatt accattaata tcggtaatcc
     1741 tatcaatata gtaatactag taacttttct aactctatat gtaaaagctc caagtaatac
     1801 tcctataaaa cctatacata tttcatttat acatatttgt acaaaataat ttaaataagt
     1861 tagtggcata cttgatgttg aaggatctat cagtgctgat atagatgtcc aaatcatatc
     1921 tctctttagt aaaactgata gttctatact gtaacctgtt ataatttcta taaataactt
     1981 cgataaaaaa atcaaaacta tacctaaaat agaaatcaca aagcttaaaa taattaaatc
     2041 tatgacaata gcttttatac aactttttcg atctgctctt atacttaaag ctcctgaaaa
     2101 gtctttattt gctatattta ctccatacac aaatagcagt atagttatat atgttaaaat
     2161 aggattcaca atcgtggata aattagtttt agatgcttct ccaaaaatct tgaggataaa
     2221 cactggcaaa atattcataa caattcccac aatcataaat ataattatat acaatttagt
     2281 atttttatta aaaaatttta tatatggctt taattcattc aattacaatt cctccttctt
     2341 agtcatgtta acaaacatat cttgtagccc tatgtatcct acatcaatat ttgaattttt
     2401 aagtattttt tcatcatttt cactaaaatc accatagtaa aaataagcaa ctgcatttcc
     2461 aaatgccttt ttaggtttta tattttttat acattctaat ttttctaact cttctttatt
     2521 tccagtaata taatgagctt tttgttttat gacatcaatt tcttcatcta taataatttt
     2581 tccctcattt aatattatta catgctccag taaatcatct atttcatcaa tcaaatgagt
     2641 tgatattatt attgtccttg gattttttat ataactatct aaaattatgt tataaaactc
     2701 ttgtctattt actgcatcca aaccaattgt tggctcatca aatatggtga tagctgaatt
     2761 agaacaaata cctattatat ttgaaagtat agttttcata cctcttgaca gttgtttata
     2821 ctttttttta gtatttaaat caaaatattt gcataattct tcttctaagc ctttatcata
     2881 acttttataa aaactagaag aaaattcaaa tatctgacct actttaaaat ctggactata
     2941 aaattctttt tccctgacga tacaaacttc ttccaaaact tttatatctt cttttgggtt
     3001 ctttcctaat atttctatct caccttcatt ttgaattaac tgatttacca ttatatttaa
     3061 taatgttgtt tttccagctc cattcttacc taataaacca tatatcttat ttttttcaaa
     3121 gtttagagac atattattaa aaatattttg cttcttaaaa ctgtgagata aattttctat
     3181 cttaatagaa ctcctcatat attaatcctc cttgaaatta attataatat caaccaattc
     3241 ttctctattt atttctaatt tgttagcttc ttgaagtagg ttttttacaa aattatgttt
     3301 aaaattttct tttctagctt cttttataat agtttttgca ccatctgata caaacatccc
     3361 tatccccctc ttcttatata aaatattttt atcaaccaat atatttatac cttttaatac
     3421 tgtagctgga ttaattttat aaagctttga aagctctgtt gttgatggaa cctgttcttc
     3481 ttcttttatt ccatttgata gtatctcatc ttcaattgct cgtgctatct gaataaatat
     3541 aggttcttca ttatttaata ctaatttcat aggttcctcc tttctccatt agttagttac
     3601 tcaactaatt aactatataa cttataatat cttgaaaaat attttctgtc aatactaaat
     3661 aaaaaacaga gggaaaattc cctctgtttt ttgttcttta tatttataat taaattttga
     3721 tttttgtaat cctatcccat aagttaaaaa ctttaataat ctgttgtatc ttcatttttt
     3781 aatattttac atattgagct agttcctaac ctttctgctc ctgcctctat aaatttttct
     3841 gcatcagaaa ttgatttaac tccccctgca gcttttattt ttacattttt tccaacatgc
     3901 tctttcataa gctttatgtc ttctaaagta gctccacctg ttcccattcc agtagatgtt
     3961 tttataaaat ctgaacctga cattgttact atttcacaca tttttatttt ttcatcttca
     4021 tctaaataac atgtctctat aatgactttt aatactttat ctcctatagc tttttttatt
     4081 tcttttattt cttttcctat attatcataa tctttatttt ttatatctga aatgtttaca
     4141 accatatcaa cttcatctgc tccattttca atagcatctt ttgcttcaaa tacttttgta
     4201 gcagtagttt gatatcccaa agggaaccct atcactgtac atatctttat tttacctttt
     4261 agatattctg atgctctttt cacatatgat ggtggtatgc aaactgatgc tacactcata
     4321 tctacagctt catcacataa aattttaata tcttcccatg tagttgtagc ttttaaaata
     4381 gtatggtcta ctgtttttaa tatatgtttc ataaaatcct cctaaattta taattaatat
     4441 aattagatta cttttttaaa tgcaattttc tctttgcaat attcagtatc atatcaacct
     4501 cttcaacttt aaaaatagtc attagtagtg tataaattcc acctccaact ataactgaaa
     4561 tagctagtga tacaaactca ttaaatgttc ctactcccaa aattccaaat ataaatttat
     4621 acgtaaagta tgataaaaca cccataatta aagatgcaac taccgatttc aaagttgctt
     4681 ttattatttt atcttgtcca aaatatccaa cttttctctt taggttcaag aagagtaaca
     4741 atatacatgc aattgatgat gaactagttg caagagcaag accaccatga gccattggtt
     4801 ttattaaaac taagtccaat actatattta cccctacaga tataatccca tttaccatag
     4861 gtgtttttgt gtcttgtaat gaataaaaaa cctttcccaa aatatctctt aatccaaatg
     4921 cagtcatacc aactgcataa aaaattaagg cagttgctgt catttgggtt gccctcgcat
     4981 caaaagcacc tctttcaaaa atgattctaa caacaggagt tgcaaaaaaa atagaagcaa
     5041 ctgaaatagg aatcatagaa attattatca tatttatact agatttaact gaactagtaa
     5101 acttcttttg attattttct gcacttaact tagataacat aggatacata acagatacta
     5161 ttgaagctgt aaatgtccct gtaacaaaac catttaatct atctgaataa gtaagtgctg
     5221 gaatgcttcc tttaactaat gttgaggcta aagttgtatc taccaatgag ttcacttgat
     5281 taacagcaac acctataaat actggtgaaa gtaatgctag taatttaata aggctatcat
     5341 ctttaaagtt taagtagtat aagtatttgt agttcgtttt tttaacaaaa aacatgtaaa
     5401 agagcagttg aactaccata gcaaccacag caccaattgg aagtatgtat ggtccaaata
     5461 cggtacttaa cattattgaa ataattatta ctatattata gggtatactc ccaaatccaa
     5521 ctacaataaa gttctcttta atttgcagaa atgctgacat aacactcgta atacctatga
     5581 atattattcc tgttatcaat attttagtaa attttacggt aagtaaaaac ctctctcctt
     5641 caaatcctat agcaaatata cttacaagtt gctttgagaa aataactcct aatatagcta
     5701 caactatgca tataccaact attatattta aaacattgtt taaaaacttt agtgcttgct
     5761 tttcaccttg tttactactt atatcttgat acattggaat aaaagtagta actatagcag
     5821 tacctattgc tgcaaatata atatttggta tattcatagc tgttaaataa ctctctgtat
     5881 ataagcctgt accataaatt gatgataata ctaattctct acccattcca agaatcttag
     5941 agattatagt aactatcatc aaataaaaag ttgctttagc taccttgctc attttatcac
     6001 tccacttcta acatacctct ttttaatcaa gtaaaatttg gctcattaga tataattcta
     6061 tcaactaatt tgccttgctt agccctatca aaatataatt aacacctatt tcttaatata
     6121 tcacaaacta cattattctt catatttttt tatttctcca ataagtttta catctttcct
     6181 atataaattg tatagcttca attatattca aagaaatgga gttgctaccc gataacttta
     6241 aaagtaaaaa acagatttta ttatcttttt aaactatatt ctaacatatt ttgtccatat
     6301 tgagaaattt tactaaacta ttatgtactt tattaaataa ataaaataac agcacaccta
     6361 taaaatacaa gtgtgctgtt cataaacaga ttttataaaa aaataattcc taagtagaga
     6421 ttaacatgct ttctcatact ctcatatata atgtgtctaa aaatattaat aaaaatgctt
     6481 taattgtttt aacttacttg gatgtcttaa ttttcttatt gctttagctt ctatctgtct
     6541 tattcgttct ctggtaactc caaatacctt tccaatctgt tctagtgttt tagggtcatc
     6601 atcatcaatt ccaaatctca ttcttaaaac ttgctgttct tgctcaccaa gacctgtaag
     6661 tagttcttct atcttttctt ttaaattatg ctgttcaact tcgtgtatta caacatcttt
     6721 aaaatcagca tctgatggta taaattcaac taaactacta tcctcatctt cttttaatgg
     6781 agtatccaaa gatacaacat ttttatctct tcttaataat tccaacactt tgtcaacttc
     6841 taagccacat gcatctgcaa tctcatctat agttggctct cttaataata catttaaaag
     6901 ttcttgtttc tttttcttta caaaatttat tctctgatga agatgtatag gtatcctaat
     6961 agtattttcg caatcgtcga tatatcttgt tatactttgt tttatccacc aagttgcata
     7021 agtgctaaat ttgtatcctt ttttataatc gtacttttct acagctttta taagtcctat
     7081 atttcctgct tgaattaaat ccaacatatc aatactatct cttttatatc tctttgctat
     7141 gctcactacc aacctataat ttgaatttat aaatttttct tttgctactg aagatgagtt
     7201 gattatttcc ttagccagct ctacctcttc tcctgctgac aacattttgt attcctctat
     7261 ttcctttaaa tacattttca tcgcatttga tacatttgat gaacccacac aaatataatt
     7321 ttcataatta ttttcttttt tagtatccat aatcaaaccc cttctattca tagaatttgc
     7381 taaaacaaaa ataaataatt taaacctaca taatctacaa ttacataata tcccattctt
     7441 taaatgaatt gttttgtata ttatgtatac aaatttataa aaactatacc tataatatta
     7501 ttctacttct aaccgcaata tcctccttat atata
//
LOCUS       DAMJVX010000166         7489 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMJVX010000166 DAMJVX010000000
VERSION     DAMJVX010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7489
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.172"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            109..1086
                     /locus_tag="PC407_003056"
     CDS             109..1086
                     /locus_tag="PC407_003056"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDF2498467.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKTHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1079..2047
                     /locus_tag="PC407_003057"
     CDS             1079..2047
                     /locus_tag="PC407_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HDF2498468.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2231..2821
                     /locus_tag="PC407_003058"
     CDS             2231..2821
                     /locus_tag="PC407_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2498469.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2840..3610
                     /locus_tag="PC407_003059"
     CDS             2840..3610
                     /locus_tag="PC407_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HDF2498470.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3747..4715
                     /gene="dusB"
                     /locus_tag="PC407_003060"
     CDS             3747..4715
                     /gene="dusB"
                     /locus_tag="PC407_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDF2498471.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4824..5303
                     /gene="greA"
                     /locus_tag="PC407_003061"
     CDS             4824..5303
                     /gene="greA"
                     /locus_tag="PC407_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDF2498472.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5318..6847
                     /gene="lysS"
                     /locus_tag="PC407_003062"
     CDS             5318..6847
                     /gene="lysS"
                     /locus_tag="PC407_003062"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDF2498473.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7156..7350
                     /locus_tag="PC407_003063"
     CDS             7156..7350
                     /locus_tag="PC407_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498474.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NIWADRVRANSYSYENKIAK"
BASE COUNT         2992 a          753 c         1512 g         2232 t
ORIGIN      
        1 aattaaaaag gaaatttaaa attaatagta ttaaaagtat attaataatg tataattatt
       61 tattagaaga aaataagtca tgaaattatg tcatattaga ggtgaaatat gcgagataaa
      121 ataatagaga ttatattaaa taataaatct gaatttattt caggagaaga attatcaaaa
      181 caattagggg tgtcacgtgc agctatatgg aaacatatga aagccttaaa agaagaggga
      241 tataatatag aatctgtaaa taaaaaaggg tatcgattag cggaaaatcc aactgattta
      301 ttaagcccac agaatattta ctataaatta gatacagagt ttattggaaa aaatattgaa
      361 catcttgaca ctatagattc aacaaatgaa catgctaaaa ggatagctat aaatagtgta
      421 gatgggactg tgattttaag tgaacatcaa gatatgggga aaggacgatt gggaagaaga
      481 tgggattcaa aaactcatga aggaatctgg atgagtatta tattaaaacc agatatggaa
      541 ccattcaaag ctccatttat aactttaata gcaggagcaa gtgtaacgaa agctttaaac
      601 aatctaggag caaatacatt aataaaatgg ccaaatgatg ttattttaaa tggaaaaaaa
      661 atatgtggta tattaacaga gttatctgct gaaattgaaa gaacaaatta tgttgttctt
      721 ggaattggaa taaatgttaa aataatggat tttgaacaag aaatagctga caaagccaca
      781 tcattgtata aagaagggta tatgttatcc agagtagata ttgttaggaa tgtattgact
      841 gaatttgaaa aattatattt agattatact gtaaataatt ccaaagaaaa aaccttaaaa
      901 atatgtagag attattctgc aattatagat aaaaatatat atgtattaaa aggagataat
      961 agagaactag tgaaatgttt agacataaat gaagagggaa atttattagt tcaaagaagt
     1021 gatggaagta taaaagaaat tatttcaggt gaagtttcta taagaggaga aaaaggatat
     1081 gtataataat attttggata tagaaggctt aaaggttggg caggttgagg atagaaatgg
     1141 tcttactggg tgcacagtag taatatgtga agaaggagct acttgcggtg ttgatgtaag
     1201 gggttctgct ccagggacta gagaaaccga tttattagac cctgtaaata tggttcaaaa
     1261 agttcatgca gtagttttgg ctggaggttc tgcatatggt cttgaatcca cttgtggtgt
     1321 aagtagatac ttagaagaaa aaggaattgg ctttgatgta ggtgtaggta aagtgcctat
     1381 agttgtagga gcagttttat ttgatttagg agtagggagc tttaaatgta gaccagattt
     1441 aaagatgggt tatagagctt gtgaagttgc aaatactgat attttaaatc aaggaaatta
     1501 tggagcaggt tgtggagcca ctgttggaaa aataagaggt caggaatatg ctatgaaagg
     1561 cggaataggt agctattcta taaaattaga taatggtcta attgtatctg cattaattgc
     1621 tgtaaatgct cttggagatg tttatgaaaa tggtaaggtt atagctggtg ctttaaatga
     1681 taaaaagaca gaaacaattg atacatataa attaatgaaa caaggtgaaa gtaaaggtgg
     1741 atttagtgta gataatacta caattgggat aattgcaaca aatgctaagt taaccaaagc
     1801 tggatgtaaa aagatatctc aaatgggaca tgatggatat gctaagtcta tttttcctat
     1861 ccatacacct catgatggag atacaatatt cacaatatca acaggagaaa tagaagcaga
     1921 tataacatta gtaggttcat tggctgtaga ggttgtggaa aaaagtataa taaatgcagt
     1981 aaaaaaagcg gatagtgtag aaaatataat ggcatataaa gatatagtaa aaaaacgaga
     2041 aatatgatga aattagcata tttttcttgt caaattgttg acaatggcaa ctcatcttga
     2101 taaaattatt cttgttagag cgggcatcct acaatggtag cttgactcat agttataaga
     2161 aaaaaacgaa aaaaattaaa aaattaagta gtccggcatc ttaatgagct ggaacggagg
     2221 tgtatttttt atgatgaaca caagtgcatc agtaagaaaa gtaaatgtaa gaaagatgac
     2281 tataatagga gtactatcag ctatttctat tatgctatct atgactccat tgggatttat
     2341 tccagttgga cctacaaaag caactataat gcacatacca gttattattg gagctattat
     2401 ggaaggacca atagttgggg cagcaatagg atttatattt gggatatcaa gtctcttaaa
     2461 tgctataaca aacccaacag taacatcttt tgtttttata aatccattag tttcaatatt
     2521 accaagagtt atgataggtg tattagctta ttatgtatat cagttaataa ttaaggctac
     2581 aaataaggta tacatatcag gtttaattac aggggcaata ggttctctgt taaatacagc
     2641 tggagtttta ggaatgatat atgtcttata tgctgacaag tatcttcatg caatgggtaa
     2701 aagtggttca gcaggaaaag taataatggc tcttgccgca gcaaatgggg tgccagaagc
     2761 aatagtaggg gctttagtag tagctgctgt tgccactgta cttaagaaaa gcaaaaaata
     2821 gaaagaaggt taatgacata tgcttctagt atttgatgtt ggaaatacta atatggtttt
     2881 aggtatatat aaaggtgaca aattagttaa ttactggaga attaaaacag atagggaaaa
     2941 aacgtctgat gaatatggaa tcctgataag taacctattt gattatgata atgtgaatat
     3001 aagtgatatt gatgatgtta taatatcatc tgtagttcca aatgtcatgc attctcttga
     3061 aaacttttgt ataaagtact gtaaaaaaca gccattaata gtaggtccag gcataaaaac
     3121 aggtctaaat ataaaatatg ataatccaaa acaagttggg gcagatagaa tagttaatgc
     3181 tgtagcaggg atagaaaagt atggagcacc aagtatactt gttgattttg gaacagcaac
     3241 tacattttgt gctatctctg aaaaaggtga atatttgggt ggaacaatag caccaggaat
     3301 aaaaatatct agtgaggcgt tatttcaaag tgcgtctaaa ttacctagag tagaattagc
     3361 taagccaggt atgactattt gtaagagtac tgtatcagcc atgcaatctg gaataattta
     3421 tggatatgtt ggtttagttg acaaaataat aagtatgatg aagaaagaat tgaattgtga
     3481 tgatgttaag gttatagcta caggtggatt agctaaactg attgcttcag agacgaaaag
     3541 tatagattat gtagatggtt ttttaacact agaaggattg agaataatat atgaaaaaaa
     3601 ccaagaataa ttaatataca gggaattggg ttatatatcc aattcccttt ttatgataga
     3661 tttctagcta tatgtggtat attataatga ggaaataaga atttaaagtt aaatcacaaa
     3721 ataaaggcaa aaataggaga aaaacaatga aaataggaaa tttagaattg aaaaataaag
     3781 tttttctatc acctatggca ggtgtaacag accttccatt tagattaatt tgtaaagaac
     3841 agggatgtgg gcttttatat acagaaatga taaatggtaa ggcactttgc tatgatgatg
     3901 aaaatactaa aaaaatgctt aagatagaag aggaagaaca tcctgtagca gttcagatat
     3961 tcggttcaga accagagttt atgggaagag cagcagagat aatgaatgat tattcaaatg
     4021 agattctcga tataaatatg gggtgtccag ctccaaaagt agttaaaaat ggagatggtt
     4081 cagcacttat gaaaaatcct aagcttgcag aagaggtatt gagggcagta gtgaagaact
     4141 caaaaaagcc agtgacttta aagattcgaa aaggctggga tgataatagt gtaaatgctg
     4201 ttgaaatagc aaagatagca gaggattgtg gaataagcgc tttagcaata catggaagaa
     4261 ctagagaaca gttttataca ggcaaagcag actgggatat aattgcagag attaagaaaa
     4321 atttgagtat acctgttata ggaaatggtg atgtatttac aatagaagat tcaataaata
     4381 tgttagacaa aactggttgt gatgctataa tgataggtag gggtgctcaa ggtaaccctt
     4441 ggatatttaa aagaataaac cattatatga atacaggcga gattttgcca gagccaactc
     4501 ttaacgaaaa aataagtact gccattaagc atttaaaact tgcagtagaa gaacatggag
     4561 agtatgtagc tgtaagggaa atgagaaaac acatcgcttg gtatttaaaa ggacttagaa
     4621 actctgcaaa attaagggat gaaattaaca aaatagaaga ctatcaagaa gttgtgtcga
     4681 aattggagta ctatatggaa gactccttga cataaaaata atagtgactt ataatatagc
     4741 gcataaatta aaaaaagatt ataaaatttt taaaatataa ttaggaaaat tcctaagaaa
     4801 tttgtgaagg agttgtattt ataatggaag aaaataaaga gtttttattg acacaagagg
     4861 gatatgataa attagaagaa gaattagaaa acttaaaggt tgtaaagaga aaagaagttg
     4921 cagagagaat aaaagttgct atatcttttg gtgatttatc tgaaaatgct gagtatgatg
     4981 aagctaaaaa ggaacaagca caagttgaag aaagaatttt aaaattagaa aatatggtta
     5041 gaaaagctgt tatcatagat gaaagcaaaa tagaccttaa tgttgttaca ataggatcaa
     5101 tagttaaagt taaggattta gaatttgatg aagaggttga atatactata gttggttcga
     5161 cagaagcaga cccatatgat ggtaaaatat caaacgaatc tcctgtaggg aaagcacttt
     5221 taggtagagc tgcgaaagaa gttgtggaag tgcaagttcc tgatggagtt gctaagtttg
     5281 aaatattaga aataagaaga taatttggag gaaaaaaatg aaaaataatc aacaaagcaa
     5341 tgaagaagcg caaattcaag aagaccttag tgaagtactt caagtaagaa gagataaact
     5401 aaaaaaatta caagagtcag gtagagatcc ttttaaagaa agtagatatg atagaactca
     5461 ttattctatg gatataaaag ataactttga ttctcttgaa ggtaaaacta caaaaatagc
     5521 aggacgtata atgagcaaga gaatacaagg gaaagctggc tttatagata ttcaagacca
     5581 agagggaaga atccaatctt atgtaagact agatgctata ggagaggaag aatatagtgt
     5641 attttcaaca tatgatattg gagatatagt tggaattgaa ggagaaatct ttaaaactaa
     5701 aaaaggtgaa atatctgtaa aggctaaatc tgtagtatta ctatgtaaat ctttacaagt
     5761 tttaccagaa aaatatcatg gattaaaaga ccaagaacta agatatagac aaagatatgt
     5821 tgacttaata gtaaatccag aagtaaaaaa tgctttttta attagaacaa aagcattaaa
     5881 agcattaaga gcatacttag atgatagagg atttttagaa gttgagactc caatattaaa
     5941 tactatagca ggtggggcaa atgctagacc atttataact aatcataata ctttacatat
     6001 accaatgtat ttaagaatag caaatgaatt gtatttaaaa agacttattg ttggtggatt
     6061 tgataaagtt tatgaaatgg gaagaatgtt tagaaatgaa ggtatggatt taaaacataa
     6121 tcctgaatat actgcaatag aattatatca agcatatgca gactatactg atatgatgga
     6181 aataacagaa aatgttattg cacatatggc tgaagttgct acaggaagta tgatagttaa
     6241 ctatcaagga acagaaataa actttacacc accttggaaa agaatgagta tggaagattg
     6301 tgttaaagaa tattctggtg tagatttttc tactataaat acagatgaag aggcactaga
     6361 agttgctaga gaaaaaggta tagagataaa acctggaatg agaagaggag aagtaataaa
     6421 tgctttcttt gaagaatttg gagaagataa attaatccaa ccaacattta taactcatca
     6481 tccagttgaa gtatctccat tatctaagag aaatgttgaa gacccaagaa gaactgatag
     6541 atttgaggca tttgctaata agtgggagtt agctaatgca ttttctgaac ttaatgaccc
     6601 tattgaccaa aaaggaagat ttatagacca attgagaaaa agagaattag gtgatgatga
     6661 agcttttgaa atggatgaag atttcttaaa agcattagag gtaggtctac ctcctacagg
     6721 aggactagga ataggtatag atagagtaat tatgttatta actaactctc catctataag
     6781 agatgtatta ttattcccga ctatgaaacc tattgataac aatccaaata aagaagaaga
     6841 aaactaagaa atattagcat tctactaagt tttaaataaa gtttagctat taaattagca
     6901 ataaatagct aaattttgtt taaaatgtca gtgtagaatg ctttttatat gcttcaaaga
     6961 ttcttttaga tatttaattt ataaattacc aacaaacgtt agaaattgat attagttttt
     7021 gttgattgag actttatatt tagaaaatat gattgaaagt aatttattat gaaaatattt
     7081 agaataattt atgagaaggt aaaaagaata atttatgaga atactatata taaaaaatta
     7141 ttgggggaat gtctgatgtt tcaagtaatt aattttttat gtttattttg tgttggattt
     7201 ttggttcttg atgttatgat atatttttgt aaggatgcaa tggatatttt tgagggtaca
     7261 actaatgtta ataaagttaa gagtataaat atttgggcag atagagtgag agctaattca
     7321 tattcttatg aaaataaaat tgcaaaatag gtaaaattat atatgcatgt attgtttgta
     7381 atatcaaggg ttatatctat tagttttaat tatatttatt aaaaaaacca aaagttataa
     7441 ccttttttaa aataaaaaac aaaaaaagta ttgacgaaat atttttaag
//
LOCUS       DAMJVX010000167         7402 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMJVX010000167 DAMJVX010000000
VERSION     DAMJVX010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.173"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            658..1167
                     /locus_tag="PC407_003064"
     CDS             658..1167
                     /locus_tag="PC407_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDF2498475.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            1195..1389
                     /gene="rpmI"
                     /locus_tag="PC407_003065"
     CDS             1195..1389
                     /gene="rpmI"
                     /locus_tag="PC407_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDF2498476.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1445..1801
                     /gene="rplT"
                     /locus_tag="PC407_003066"
     CDS             1445..1801
                     /gene="rplT"
                     /locus_tag="PC407_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDF2498477.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            2111..2656
                     /locus_tag="PC407_003067"
     CDS             2111..2656
                     /locus_tag="PC407_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498478.1"
                     /translation="MNEKLMDCFVNPIKCKLLLEVFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKEGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            2643..2816
                     /locus_tag="PC407_003068"
     CDS             2643..2816
                     /locus_tag="PC407_003068"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498479.1"
                     /translation="MKGNKSDLHEVEPIYTENRFMIYICIQSKVSLHGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            2816..2992
                     /locus_tag="PC407_003069"
     CDS             2816..2992
                     /locus_tag="PC407_003069"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498480.1"
                     /translation="MPKEMNFRPHMQLGYIIQLDQIYRIKISIFIADKLTVILLKNHL
                     LVIVSILKEIIVIH"
     gene            3092..5017
                     /locus_tag="PC407_003070"
     CDS             3092..5017
                     /locus_tag="PC407_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2498481.1"
                     /translation="MKIKFVIMPLVIFLIVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFISNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            5418..7322
                     /locus_tag="PC407_003071"
     CDS             5418..7322
                     /locus_tag="PC407_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2498482.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFKQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEGKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         3123 a          734 c         1384 g         2161 t
ORIGIN      
        1 taaagtatta taaagcatgt attgattaag tatttattaa gtattgaata aaatattaca
       61 aatttagttg aaatgaaaat gataaaaaat gttgtgctat attttacaaa tcatataaat
      121 agagaaattg ttgtagcaca aagtaatagg tagtatatta gaatataata taggatttgt
      181 tgtgtagtat agtagcatat tagaatataa tataggattt gttatgtagt atagtagcat
      241 attagaatat aatataggat ttgttatgta tatagtagca tattagaata taatgtagga
      301 tttgttatgt agaatatata gagtcatgta gaatataaat atataaaagg attttgggta
      361 taaatttaaa aattaatagc aaaatgagta gtatagacat ataattagta gtaggaagta
      421 attaaatttt tcctaaacaa aaaaaatagt tgcaaataat ttgattaagt gatatagtaa
      481 ataaggtaag aataaaacaa agaagaagtc cgcttctcac cttacgccgg aggttagtaa
      541 ggtaaatata agttaaagct taattttaat ttaagtttgt ttaaatttgg taacttatat
      601 tgcggatgat tacgtcatcc gctttttatt ttataaaaat tttggaggtg tcctacaatt
      661 agcaaagaat tagcaatcaa tgaacaaata aaagataagg aaataagagt cctttcacca
      721 acaggtgaac aacttggagt aatgcctact aaagaagctc aagcaatggc taatagcaaa
      781 aacttagatt tggttcaaat ctcgccgaat gcgaatccac cagtatgtaa gataatggat
      841 tatggaaagt ttagatatga gcaagcgaga aaagagaaag aagctaagaa aaaacaaaaa
      901 actatagttg taaaagaagt aagacttaga ccaggtattg aacaaaatga tttaaatacg
      961 aaagctaata atgctataaa gttccttaaa aaaggggata aagtcaaagt tgaacttaga
     1021 tttagaggaa gagaattagg acataaagac ataggtaaag aagttatgct taagttttta
     1081 gatataataa aagagtttgg agaaccaaca aaagcccctg catttgaggg taataatatg
     1141 gttgtaatta tagatccaaa aaaatagatg attaactgag aggaggaatt tgtcatgcct
     1201 aaaatgaaaa ctcatagagg tgcagcaaag agattaaaga aaacaggaac tggaaagtta
     1261 aagagagcta aagcttttaa aaaacatata ttaactaaaa aatctgcaaa aactaaaatg
     1321 aacttaagaa aatcaacttt agtaagtgat ggagatgcca agagaatagc acaattatta
     1381 ccatactaag atacagattg taaataataa ctatagaaga gggatataaa aggaggtcat
     1441 aacgatggca agagttaaaa aagctatgaa cgctcgtaaa aagcataaga aaatattaaa
     1501 acttgcaaaa ggattcagag gatctagaag caaattatat agaccagcaa atacattcgt
     1561 aatgaaagca ttaaaaaatg cttatatagg aagaaagtta aagaagagag acttcagaaa
     1621 actttggata caaagaataa atgcagctgc tagaatgaat ggaatatctt attcaagatt
     1681 aatgaatgga ttaaaattat ctggtattga agttaacaga aaaatgttat ctgaaatggc
     1741 tatacaagac ccagaaggat ttgcaaaatt agcagaagtt gctaaagcaa aattagctta
     1801 gtattgaatc atgagatgta aagtctttga gttatataaa taattcaagg acttttttat
     1861 tttttgaaaa atacttatta gtgtcatgta caaccaattt tataatgata aataaaataa
     1921 gcaatacaat agaattttat gagatttatt ataagaattg ttacaattat ttatcatagc
     1981 tataaaaagt catagtaaaa atgatattga tgtatgttta tatatgtata aagctttcaa
     2041 tcaatcttga attaatatta tcaacatgat aatattatct tattaataat ataaataagg
     2101 gggagataat atgaatgaga aactaatgga ttgttttgta aacccaatta aatgcaagtt
     2161 attgcttgaa gttttttcaa ataatcgaac tacagctaaa caattaggag aaaaatttca
     2221 agatatatca aaggcaacac tttatcgtta ccttaataaa atgttgaaag atgatattat
     2281 aaaagtggtt gaagaaaata aaataagggg aactgttgaa aaagtatatg ctatgaagtt
     2341 agatatagct gatgatgtga acaaaattat agatgagaac tctggagaag aatatgctca
     2401 actgtttact caatatatga tgggattttt aagagaattt tatgaatacg ctgaaagaaa
     2461 ggaaaaggat atcctaaaag agggttcagg attttctgtc tgtccagtat atgctagtat
     2521 tgatgaaata gttgaagtga gtaaaaaaat tgctagtata ctagaacctc taagaaataa
     2581 ctcgcacgaa gaaggcagaa gaatgcattc tataggagtg ataatcactc cacctaaaga
     2641 ggatgaaggg aaataaatct gatttacatg aggtagagcc aatttacacg gaaaatcgtt
     2701 ttatgattta catatgtatc cagagcaaag tatctctgca tggattgtac ttcattgtac
     2761 catttcatcc aatgacttgg agtggaacaa tgaagttttt taatttttca tattaatgcc
     2821 taaagaaatg aactttagac cacatatgca attaggctac attatacaat tagaccagat
     2881 atacagaata aaaattagta tttttatagc agataaattg actgttattc ttcttaaaaa
     2941 tcatctatta gttattgtta gcatattgaa agaaattata gtaatccatt gaataaatga
     3001 atattgccca taatcatata aactgctata gtttaaaata aagcaggacg ttaaatttta
     3061 tagtaatctt aaaatttaag agggggattt tatgaaaata aaatttgtta ttatgccatt
     3121 ggtgatattt ttaatagtat tatttgtggg gtgtcaaagt acaaaagata cagaagttaa
     3181 agcaaaagat acagaaatta aaatattagg tacaggtgat ttgcattcaa ttttgacaga
     3241 ctctatggtt tcatatgtaa atgaggaaag ggaaaagaat aaaaatctgc ttatggtaga
     3301 tgcgggagat ttctatggta cacaaagtag agaaatgtgg gaatggtctt ctggaaaaaa
     3361 gttaattaat attaaaagaa atggtagagc agagtatgat gacataatta aaagtagtaa
     3421 agatgaagta cctatagtta aagatatggc aaagttgaaa tatgatgctg tagttttggg
     3481 agataatgaa tttatttcaa atgataagca aagtcttgat aaactagttt ctgattttaa
     3541 aaataataat atgccattgg tatcagcaaa tatatatgaa caatctggag aaaattatgt
     3601 acaaccatat gtaatgaaaa atattaagac agatgagggc aatgtaaaag ttggaatact
     3661 aggtcttact ataaaagaag taggggaaac ccttggtctt gaggattttg aaaaggataa
     3721 aaaagctaga gagcttggag aacaagctga ttacaaagga aaattatatg caaatgattt
     3781 agtagaagat gcaaagaaat ggataaaagt tatggaaaaa gaaagtccag acataatagt
     3841 tgcagttgta cactcagggg aagaacctaa aacagataga aatccaggaa atagaataaa
     3901 agagttagca actacagtgg atggaataga tgcaattgta gctggtcata ctcatgaaaa
     3961 aatagatgaa cacaaatata aaaataaatc tggagatgaa gtaattgtta cacaaccagg
     4021 ttggcatgga gatagagtat cagagattaa tttcaaatta aataaagaga atggaaagtg
     4081 ggacataaag gaaaagtcta gtaaattaaa aataattgat aaagaagtaa gtatagttgc
     4141 tactgcagat ttacattcac atttctctga taaattaaca gttgaccttg ttaatgaacg
     4201 tagtaaccca attgaacctg ttgtagtaga tgcaggcgac ttcttagacc ctcaaacaga
     4261 tgaaatgact caatggtata aagagtggaa aagtataaga gataataata gtgataaaac
     4321 gattagtaga tgcccaatgg tcatggatat gaatcaggga atgtatgatg cagtagtact
     4381 tggaaatcat gaatttgtat ctgaaaaaga tgaattttgg tcagatgaac gccttttaga
     4441 tgctgttata gaggattttg aacaggtggc aatttctgtg ttatcagcaa atatatatag
     4501 gcaatctggt aaaaattatg ttaaaccata tattataaaa gatgtggaga cgagtgaagg
     4561 taatgtaaaa gtaggaatat tgggacttac tataaaagaa gtaggtaaag gtcttaaaaa
     4621 tgtaaactta caagagcaat ttcaatataa ggataaattg tatgcaaatg atttagtgga
     4681 agatgcaaag aaatgggtaa aagttatgaa aaagaaaaaa ccagatataa tagttgcagt
     4741 tgtacattca ggagaagaac caaaaaatcc aaaacaccca ggaaatagaa taaaagagct
     4801 agcaactaca gtagatggta ttgatgcaat tgttgctagt catactcatg aaaaaataga
     4861 tgagcatgaa tataaaaata agtctggaga aaaagttata gtgacacaac caggtgagca
     4921 tggtaaatat tattctaaaa ttacctttaa agtaaataaa gaaaagggtt cgtggattat
     4981 taatgataaa ttcagtaaaa caattgaggt tgaataactt tataaaattt atagataaag
     5041 taaattatct gatattgaca tgatatataa gataaagttt ctaatattag tataatgcat
     5101 aatgtgaaat attttaataa taagagaata aacaagacaa ataaaaatat tttaacatgt
     5161 aagatatagt atgaactatt atactaatgc ttactttata atttgaacat gttagaataa
     5221 gtcttatttg aaaattttag tgtattagag taagtattat tttaccagta aaaaatattg
     5281 agaactatat gtagatttgc ttaaaaagga atttacatat agttcttttc gtataataaa
     5341 tagtacaatc ttatatggta aagataaagt gtgatattaa attttattat catccaaaat
     5401 ctaaggagga gtctcttatg aaaataaaat ttattatagt agcatcaata atatttttaa
     5461 tagtattact tgtggggtgt caaagtacta aagacacaga aatcaatgta ctcgccacaa
     5521 gtgatttaca ttcaattatt ccagataatt taatttccta tgtagaagag gagagaaaat
     5581 atgatgagaa tctacttatg gtagatgcag gagatttttt tgacatgcaa agtggtgaga
     5641 tgaataagtg gtttactggt caaaaattag taagatttaa agatgggata ccagagtaca
     5701 aaaaaatagc tgaaccacgt gaaggagaag ttccattggc taaaaaaatg gcaaagctga
     5761 agtatgatgc agttacattt gggaatcatg aatttgtatc taatgacaag cagagccttg
     5821 ataaattggt ttctgatttt aaaaataaca atataccatt agtatcagca aacgtatatg
     5881 aacaatctgg agaaaactac gtaaagccat atgtaatgaa aaatataaat actgacaagg
     5941 gaactgtaaa ggttggtata cttggtctga ctataaaaga agtaggggaa cgttcaaggt
     6001 ttaaaagttt taaacaagat aaaaaggcta gagaacttgg ggaacaacct caatacaaag
     6061 ggaaattata tgcaaatgat ttagtagaag atgcaaataa gtgggtaaag gttatggaaa
     6121 aaaaagaaaa gccagatata atagttgcca ttgtacattc aggtgaagag ccaaaaaatc
     6181 ctaaaaatcc aggaaataga ataaaagaac tagcaacaac tgtagaggga atagatgcta
     6241 ttgtagcagg tcatacacat gaaaaaatag aacagcatac atataaaaat aattctggtg
     6301 aagaagtaat agtaacacag cctggagctc atggtaatag tatatcaaag attaatttta
     6361 agttggataa gaagaatgat aagtggttta taaaagacaa atatagtgaa ttaaaggttt
     6421 ttggaaagga agtaaatata attacaactt ctggtctgaa tccaaaattt tcagatgaaa
     6481 tggcagttaa tttgtttaat gaaagagata gagatgttca acctatcttt atagatactg
     6541 gagatttttt aaatactaat acaaaagaaa tgactaagtg ggctaaagag tggcaataca
     6601 atgaaaataa aggcatctat aaaaaaataa atgaatggcc aattgtatat cttggttatg
     6661 atgctgtagt acttggtagc catgaattta cagaggataa agagactttt gagggaaaaa
     6721 aatctacatt ggattatatt atagaaaact ttgaagaaat gaagatacca gtattatcag
     6781 ctaatatata tgatgaatct ggagaaaatt atgtaaaacc ttatataatt cataatgtag
     6841 aaactaggga aggaaatata aaggttggag tattaggtct tactactact gataaggatg
     6901 aagaagactt aagtagctca agcttaagca aagactcaaa gacttcgaaa attcctgaag
     6961 aaacaggaaa gttatatact aataatctag taaaagatgc aagcgaatgg gtaaaagtta
     7021 tgcaaaagga aagtcctgat gtgattgttg cagttgtaca ttcagataat aatagtactc
     7081 taaaaaatcc aagtagtgaa gtcaaaaaac ttgcaatgtc agtagatgga atagatgcta
     7141 ttgttgtagg tcatacgaaa aataaggtag aagaacatat ttacaaaaat aagtctggag
     7201 atgaggttat agtaacacaa tctggagaag attgttattc aaaaataagt cttgaactaa
     7261 gaaaagaaaa tggcaagtgg aatatcataa acaaatatag taaagcaata aaaatgaagt
     7321 gaatttattg atatataaac cattattaac actgattttt ataatataat aaatactgat
     7381 tctacaatat aattaacact ac
//
LOCUS       DAMJVX010000168         7351 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMJVX010000168 DAMJVX010000000
VERSION     DAMJVX010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.174"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(8..955)
                     /locus_tag="PC407_003072"
     CDS             complement(8..955)
                     /locus_tag="PC407_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2498483.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKAKYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDFVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEAIKKIK"
     gene            complement(924..1370)
                     /gene="lspA"
                     /locus_tag="PC407_003073"
     CDS             complement(924..1370)
                     /gene="lspA"
                     /locus_tag="PC407_003073"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF2498484.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRVIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(1680..2459)
                     /locus_tag="PC407_003074"
     CDS             complement(1680..2459)
                     /locus_tag="PC407_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2498485.1"
                     /translation="MKKAIWGICICLLSIVGVYTYNHNTHNQNSSKETYTLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQILDILKKNNVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYGSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDTIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(2509..2991)
                     /locus_tag="PC407_003075"
     CDS             complement(2509..2991)
                     /locus_tag="PC407_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2498486.1"
                     /translation="MDTKKYKDKLLKEREKLTNLVVDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDSALSKIENGTYGFCDLCHNQI
                     DLERLDILPDTNLCNNCAKKENALLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGTLRD
                     "
     gene            complement(3175..4596)
                     /locus_tag="PC407_003076"
     CDS             complement(3175..4596)
                     /locus_tag="PC407_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2498487.1"
                     /translation="MVSFLGSIVVLIVGYFIYGTFVEKVFGINDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGIVVAI
                     ILGLLFFKKAKDIKEQNAHKLAS"
     gene            complement(4742..5512)
                     /locus_tag="PC407_003077"
     CDS             complement(4742..5512)
                     /locus_tag="PC407_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2498488.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSSIEVVNEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYENYAVDAFSLDVFDYVLKP
                     YSEERIISMLNKLEKSEMSDIELSNSNSNLYKYKKETVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(5517..6719)
                     /locus_tag="PC407_003078"
     CDS             complement(5517..6719)
                     /locus_tag="PC407_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2498489.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIVGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRTDNGT
                     LIVFYVKE"
BASE COUNT         2456 a         1287 c          734 g         2874 t
ORIGIN      
        1 caaatggtta ttttatcttc ttaatagctt ccttaaaata ctcaggtaat tctgaattaa
       61 attcgatata ttccttagtg gtaggatgta taaatcccaa cttcttagcg tgcaaagttt
      121 gtccttttaa tttaaattta gtttcttttg gaccatatac aaaatcacct aaaagtgggt
      181 gatttataga caaagcatga actcttattt gatgagttct accagtttca agcctagctc
      241 tcatatgtgt aaacttatca aatctttcta caacttcaaa atgagtaatt gcattttttc
      301 catcctttac tacagccatt tttaatctat ccttaggatt tctccctata ggtttatcaa
      361 cagttatttt atcttcttta accacaccat agcatataaa ttcatactct ctagttatag
      421 tatgttcctt taactgttct gctagaaaat tatgtgcata attgttctta gctatcataa
      481 gaagaccaga agtatctttg tctattctat gaactatacc aggtctaata actccattta
      541 ttgacgacaa cttatcttta caatgatata aaatcccatt tactagtgta ttttcatagt
      601 ttccaggagc gggatgaaca accatatcct ggggtttatt tattattaag acatcatcat
      661 cttcatatac aatatctatt ggaatatctt gaggtaaaac ctctaatact ttaggttctg
      721 gtatctctat aacaatatta tcttcctctt taactagata tttagctttt tctactttat
      781 tattgacctt tacttttcca tcttttataa ttttttgtat ataacttctt gacatatcac
      841 ctaattgttc tgctagataa atatcaagtc taacttcttc ttcctcttca agtactaaaa
      901 actcttttac ttcatccatt atatcaccta cttttacttt caaaaaataa aacatatata
      961 cataaaaaca cagttcctac aactacaaat acatcagcta cattgaatac atattcccat
     1021 ataactctaa agtcgaagta atctaccaca aaacctaatc gtactctatc aattagattt
     1081 cctaatgcac cagatataat taatataatt cccaaccttc caaatatatg tactttcctt
     1141 gtattaagat agtataatcc aaatactgtt gcaagtaatg caacaattat aaatatccac
     1201 tgattatttt gtaataatcc aaatgctgca cctctatttt cgacataagt taaatgaaat
     1261 acattattta ttattggtat tgttgaaaca tctaccaaat tattcaatac ccatatttta
     1321 gacagttggt ctaagcctat gagtagaatt attattaata tatatagcaa tgatttttcc
     1381 ccctataaat tatttaaaaa ccaattttat ttgaaacaca ctattattat tatacaaaaa
     1441 aaatgctcta aagagcaact ttattaaaat tgtattttct taataaagct agagcaaaat
     1501 ttaaaatagt accatctttt ttatattgca gattgctaca tatttaaaat catattatta
     1561 tgtataaaaa atttaaaaat aacatactaa ttaatacttt ttgtgtgttt tatagattaa
     1621 agattaatga tgtctgtact ttaaacttac aagcaccact aatcttcaaa caaatatttt
     1681 tacttttgca cttgcttatt tttatctaaa tgttgtataa gttcagatac tgttacgaat
     1741 ttatatcctc tctcttttaa tttaggaatt actacttcta aagcttgtat agtttgtgat
     1801 ttatcatttc ttttagtgtt ataatcatgt aaaagtatta tattaccatt ttggaccttt
     1861 gtaagaatag tatttataat tgtatctaca ccagggttag accaatctct agggtctaag
     1921 tttgaccaaa gaattatatt catgccttta gaaactataa tatcacagac acttttactc
     1981 atggctctat atggaggtct aaatatcttt ggttctactc ctattacact ttttataata
     2041 ttttgtgtat ctgttatttc tttttcaaca ctaccataac tattttttgc aacatttata
     2101 tgtgtaaatg tatgattccc tatttcatgc ccctcatcat attgcctttt taataattct
     2161 ttgttatatt ctactttttc tccaactaca aaaaaagttg cttttacatt atttttcttt
     2221 aatatatcaa gtatttgtgg tgtaaaatcc tcatctggac catcatcaaa agtcaatgct
     2281 agaatctttt catcctcatt tccttttttt attattacat cttcataatc gtcttttata
     2341 ttgtcattta aagtataggt ttcttttgaa ctattttgat tatgtgtatt atggttatat
     2401 gtatatacac ctactatgga taataaacat atacatatac cccatattgc tttcttcatg
     2461 tcattacctc ttttctaatt cattctaaca atatatgatg tcaggcttct aatctctcaa
     2521 tgttccattt ttatttaaat cagttaaatt tgttaggtct tctgaataaa agttagaatc
     2581 ttctacatca ggattttgca ttgcatcacc taataaagca ttttcttttt ttgcacaatt
     2641 gttacataaa tttgtatcag gtaatatatc taatctttct aaatctattt gattgtgaca
     2701 taaatcacaa aacccatatg ttccattttc tattttagat aatgctgaat ctatttgata
     2761 cagtcttcct tcttcatgat ttattaaact attttgcata ttttgcatat ataaatccgt
     2821 tcccatatct ccaatatgat tgtcataact agataattct cctgatgaat atttttcact
     2881 agtatgttta gtagtgtctc caaacaaggt attatctttc atatctacga ctaaattagt
     2941 taacttttct ctttctttta ataatttatc tttgtatttt ttagtatcca ttttcaacac
     3001 ctctaatttt aatatttata tcataaaaat agtatgtgga aaactaaatt aaaaatacaa
     3061 taaaaaacaa gatttgattt tttcaaaaaa aattgtaggt aaacttaaaa ttcacctaca
     3121 aattctttac atatattttt aatataatgt ttttagtttt tgataataat tacattaact
     3181 tgctaattta tgagcatttt gctcttttat atccttagct tttttaaaaa ataaaagtcc
     3241 aaggattatt gcaactacaa tacctattcc attagatata ttaactggaa gtctaaatcc
     3301 ttcttttgcc tgcattatat aactaaatgt tactacagtc ataaaaagtg ctggcaatgt
     3361 agttatgtaa tgatttttat ttgctttgac taaatatgca gaaccagtcc acaacattat
     3421 catagcaagt gtttgatttg cccaagagaa atatctccaa attatattaa aatctataaa
     3481 tgtaagggca acccctacag caaataatgg caaagctatt aaaaatctat ttttaactgc
     3541 atcttgatta taattcattg catcagctat tgttaatcta gcacttctaa atgctgtatc
     3601 ccctgaagtt ataggacatg ctacaacacc aagtatggcc aaagcgcctc caataggtcc
     3661 cattaatcca acagatactg tatttacaac agttgctgct ccaccatcat taaatattac
     3721 attaagctga ggtataccat ggaaatatga catagctgct gctgcccaaa ctaaagctat
     3781 tataccttct gcaaccattg acccataaaa tactggtctt gcctcactct ctttctcaac
     3841 acatctcgcc ataagtggtg attgagttgc atggaaacct gatattgctc cacatgctat
     3901 tgttataaat aagtatggga ataaaggtgt tccatttgga ttcatatttt gtaaatttat
     3961 atttggtatg ttatacccct ttattattaa tcctccagct acacctactg ccataattaa
     4021 taatgctgct ccaaataaag gatatatttt accaatcaat ttatctattg gtaaaacagt
     4081 agccataata taataaagta ttatcactcc aacccatatt tgtgcgttta cacctgtcaa
     4141 tgtctttaat aatcctgctg gacctgttac aaatacaact ccaaccaata ctaaaagaat
     4201 aacactaaat attctcattg ctatttttgc accatttcct agataaagac ctaccaactc
     4261 tgatgcagat gtacctttgt tcttcattga taaatatcct gaagcaaaat cgtgtactcc
     4321 acctgcaaag attgttccaa acacaatcca caaaaatgct gatggtccaa ataacgcccc
     4381 ttgtatagct ccaaatatcg gacctaaccc tgctatattt agaaactgaa ttaaaaagat
     4441 tctcttccat ttcattggca cataatctac tccatcccta caagctatag ctggagtttg
     4501 gtttttgtca ttgattccaa aaaccttttc tacaaaagta ccatagataa aataacctac
     4561 tataagtact actattgaac ctaaaaagct taccataaat tcatcccctt agtttttata
     4621 taattgcttt ataaattttt atgagtttaa gaaaactatt tccagttcaa atagtaatac
     4681 ttttttatgc taatataaat attaattaca ttaagtgcat atttaagtac atgaaatgac
     4741 tttatatatg cattaacagc ctaaattctt ttactttgct tctgcttaca gttacttcat
     4801 agtcactatt ttttaatttc aatatgtatg tattgttaaa ccatggtata atttctttta
     4861 ctttggttaa atttacgata taagacctat gtgttctaaa aaatgtttta tcatttataa
     4921 gattctctac ttctgatatg ccttctttta atataaactt ttctttctca gtatatataa
     4981 atgtctgcct ttcattagct tcacaataat atatatcatc aatatctatt acaaccaatt
     5041 tatcaccttt ccaaaggcta attttatgtg ttatttcttc tatctcctga tttactgtct
     5101 cttttttata tttatataaa ttactattgc tattagacag ttcaatatca ctcatctcac
     5161 ttttttctaa tttattcaac atagaaataa ttctctcttc agaatatggc ttcagtacat
     5221 aatcaaatac atctaaagaa aaagcatcta ctgcataatt ttcatacgct gttataaaaa
     5281 caattttagg ccttgattta aactgattta atgtttttgc aagaagcatt ccatcgagat
     5341 gaggtatatt tatatccaaa aaaattacat ctattttatt ttcctgaata aagtctaaca
     5401 cttctatacc attagtaaat tcatttacta cctctatgct acttttattc tcaataaaat
     5461 atcttaattc ctttcttgcc ggaaattcat cttccactat tatagctctc atacttctac
     5521 tcctttacat aaaatactat caaagtccca ttgtctgttc ttcttatatt aagccctctt
     5581 ccatacatca gtttaagcct taaatgtaca ttataaagtc caatcttatt ttctggcata
     5641 ttatcttgat atacattatt tataatttcc tcacttatac ctataccatt atcctctatt
     5701 gatattctca cagtattttc taatgaataa ggtaactttt ttatggatat tttcactaca
     5761 ccagctctac cactttctaa aataccatgt attatcgcat tttccactaa tggttgtata
     5821 atcaaacttg gaatctttat atttacatct tctatatcat aagatacagt tagtaattct
     5881 ccaaacctag ctttttctat ctctacaaaa gatttaactt gttcaatctc tttatttata
     5941 tcaattaagt tatcactata ttcaagatta tatcttagat aagttgataa atttattatt
     6001 aactctctag ccttatctgg actaaatctt ataaatgaag ctattgtgtt tagggaatta
     6061 aacaaaaaat gcggattaat ttgtctttga agtgctttta tttctgcttt atttctcgct
     6121 tctatatctt ttttatcttt ctcaatcccc tctactatag atataacaaa tcctattcca
     6181 atctgtccaa caattaaagg taaatatatc ccaacaatta tttgaattgc taaagaatgt
     6241 ggatatgata ttaagtatat aagtagtata gtaaaacttt cagaaatcat acctaccaat
     6301 ataccataca ttactctatg ttgctttggt atcctcttat atagaaatcc tgataataca
     6361 cctgctagta tacttgataa aagacatgga acagatgtta tccccccaat atccataaaa
     6421 tatctatgaa tccctgaaaa aactcctgct gtaataccta ccattggacc aaataaaatg
     6481 ccacctgata ctatagatat tattctagtg tttactattg accccatata atttatacca
     6541 ctataagttc caaatatggc taaaaaagta aaaactaatg ctatacagac caagtcatta
     6601 aaactatatt cttccttttg aaatatctgc ttaaatattt ttaactttgt tattagaaga
     6661 aacagtacta caatgaggct tgtcttttct attaatgcga ttaaaattga tgttttcata
     6721 ttccaccttt aaaaaataaa tttttacaca ctctaaattt attatgtttg ttgtaactta
     6781 tatatttgaa gtaatcctat ctcgacattt tccacattta accttttaat taaataaaaa
     6841 atggatatac ttctattaac aactaatact agctgactaa cacaagcttg taataacttg
     6901 tagttatcat taactctaca attctagtat ttaattttat actttagaaa tctcttttct
     6961 tagaagtaat actttcgaat attatataat acaatgttat gtagattctt actcatacaa
     7021 catatttctt taaaaatttt atactattct ttgttcaaat atctcatttg atttaattgt
     7081 gctaaacaca cattatcatc tctctgttat atatttatta tatagccgta tatcgataaa
     7141 gtaaataaat cctatattta aaatcatatg tttgatagta aatcattata atgtatattt
     7201 accataataa tttgccaacc ttgaaagtca cttctataat tagaagttaa ataattcttc
     7261 taaaaaaaat acctctaaat tttattttct gaaatacata gttataacta tttttatcac
     7321 gtataagaat aaaaattcca gaaaattttt a
//
LOCUS       DAMJVX010000169         7306 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMJVX010000169 DAMJVX010000000
VERSION     DAMJVX010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.175"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            216..593
                     /locus_tag="PC407_003079"
     CDS             216..593
                     /locus_tag="PC407_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2498490.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            586..1620
                     /locus_tag="PC407_003080"
     CDS             586..1620
                     /locus_tag="PC407_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2498491.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENAVGNNITQIDISTDNNLMLYVAYLWLVVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDVNCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLTDCSLKDKKEYALALIKSIKLSKNYNSGILTTEFNKTSLE
                     KRLDSIVKKKGKNGILVLLILITLFSVTYFNFESISFDNSNSLRNMGILQKSGVDLKE
                     EDDISMRVIN"
     gene            1686..2273
                     /locus_tag="PC407_003081"
     CDS             1686..2273
                     /locus_tag="PC407_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF2498492.1"
                     /translation="MKNLKKILIITGCLTLISTSLVFANTTTDVDTSKDDDSIISSCT
                     ITGYEFTYENAPQSVKFDYEKKCEALNIKPSKNDIIFVNKDSIDANDVENYVEPKAYI
                     IRYKKGYVFEVTGPKSYTLNVSNTVVGYNRVESGNPVHLVQLLCRTLDGSSIDDDSEF
                     GPATYNAVKSLQGRLGLTRDGIVGRSTWDAAGRRL"
     gene            complement(2651..3412)
                     /locus_tag="PC407_003082"
                     /pseudo
     CDS             complement(2651..3412)
                     /locus_tag="PC407_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612258.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4020..4397
                     /locus_tag="PC407_003083"
     CDS             4020..4397
                     /locus_tag="PC407_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2498493.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDATVTSKDVIEVMEQKYGWKQTTT
                     LTLLSRLVKRGFLNAQKIDRYTHYTVIVKHKDYLSFETKDFLSNIHDDSLKSLISALH
                     DDENIGKDTLDFLESYFDNLKNE"
     gene            4390..5424
                     /locus_tag="PC407_003084"
     CDS             4390..5424
                     /locus_tag="PC407_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HDF2498494.1"
                     /translation="MNNYMLYVFDNLIKTTIVCSFGICLLLFLKRYFFKRFSKRFNYY
                     IWLVIVFRMLLFLFNYTVMYEVKEPKENTVGNNITQIDVSIDNDFMLYLVYLWLFVTI
                     AIAVYTFMEYTKFKNLVVDVSYDIEDHDVNCIYKNLLKELDIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYTYSKDELTWILKHELMHFKNKDILIKFLVLSVKIMYWFNPLVYI
                     MSNKVNLDCELCCDESVLTDCSLKDKKAYALALIKSIKLSKNYNSGILTTEFNKTNLE
                     KRLDSIIKKKGKSGILMLLILFTLFSVTYFNFESISFDNSNYLRNIGILEKTGADLKE
                     EDDISMRVVN"
     gene            5903..6076
                     /locus_tag="PC407_003085"
     CDS             5903..6076
                     /locus_tag="PC407_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF2498495.1"
                     /translation="MHLVQLLCQQINGSDIDPDSKFGPATYNQVKILQGRLGVTKDGV
                     VGKATWQAVGERY"
     gene            complement(6161..7183)
                     /locus_tag="PC407_003086"
     CDS             complement(6161..7183)
                     /locus_tag="PC407_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2498496.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTLLNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNSGNGIVFIKPWNNSKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTKLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
BASE COUNT         2781 a          867 c         1029 g         2629 t
ORIGIN      
        1 ttttaatgtg taaataacat tctacactat tgtaattaaa tatgtaaaaa gtaaaatatt
       61 taaatagata tattttaaaa tctaactttt aatattgtaa aaaaattaga aatttaacaa
      121 aaagtacaaa aattcacatt tttttcataa agtgtttgac taaaaaattc acatgttgta
      181 gaattaaggt agcaggagtt ttgagggagg tgtaaataat gataagaaaa ataccacaag
      241 cagaactaaa agtaatgaaa ttcatttgga aagtagatgt tacagtgaca tcaaaagatg
      301 ttattgaagc tatggaacaa aaatatggct ggaaacaaac tactacactg acacttttat
      361 ctaggctggt aaaaagagga ttcttagatg ctcaaaaaat agacagatat acacattaca
      421 caataagagt aaaacacaaa gattatttaa gttttgagac aaaggatttc ctaagtaata
      481 tacatgatga ctctttgaga agcttaattt cagcattaca tgatgatgaa aatataggta
      541 aagataagct agattttcta gaaagttatt ttgataactt aaaggatgaa taattatatg
      601 ctatatgttt ttgaaaatct tataaagaca actattgtat gtagtcttgg tatttgtcta
      661 ttattatttt tgaaaagata tttatttaag aagtttagta agagattcaa ttattatatt
      721 tggttgataa tagtctttag aatgttactt tttctattta attatacaat agtgtatgaa
      781 gtaaaagaac ccaaagaaaa tgcagtagga aataatatta cacaaattga tatttctaca
      841 gataataatc ttatgttgta tgtagcttat ttatggttag ttgtaactat agtcattgca
      901 gtttatacat ttataaagta tacaagattt aaaaatctag ttgttgatgt atcttatgat
      961 attgaagata atgatgtaaa ctgtatctac aaaaacttgt tgaaagaatt aaatataaaa
     1021 aagaaaattg aattaagagg ttctgatgaa ttgataagcc cagcaggcat gggattattt
     1081 aaatcttaca tattcttacc agactatcct tatagtaaag atgaactaac ttggattttg
     1141 aaacatgaat taatgcattt taaaaataaa gatattttaa taaagttttt agtgttatct
     1201 gttagaataa tatattggtt taatccactt gtttatgtaa tgagcaataa agtaaatcta
     1261 gattgtgaat tatgttgtga tgaaagtgtt ttgactgatt gttctttaaa ggataagaag
     1321 gagtatgcat tagcacttat aaagtcaatt aaacttagta agaattataa tagtggtatt
     1381 ttaacaacag agtttaataa gacaagtctg gaaaagagac ttgacagtat tgttaagaaa
     1441 aaaggtaaaa atggaatatt ggtgttattg attttgatta cattattttc agttacatat
     1501 tttaattttg aaagtattag ttttgataat tcaaattctt taaggaatat ggggatttta
     1561 caaaaatcag gagtagatct taaggaagaa gatgatatta gcatgagagt tataaattaa
     1621 tagattaatg tatgtaatga aaaggtatat tgtcgtatta ttaataaatt aggaggaatt
     1681 atgaaatgaa aaatttaaaa aaaattttaa taattacagg ttgtctaact ctaatatcta
     1741 ctagtttggt atttgcaaat actactactg atgtagatac atcaaaggat gatgattcaa
     1801 taataagttc atgtactata actggttatg aatttacata tgaaaacgca ccacaatctg
     1861 taaaatttga ttatgaaaaa aaatgtgaag cgctaaatat aaaaccatct aaaaatgata
     1921 taatttttgt taacaaagat tctatagatg caaatgatgt agaaaactat gttgagccaa
     1981 aagcatatat tattagatat aaaaaaggtt atgtttttga agttactgga cctaagagtt
     2041 atacattaaa tgtaagtaat actgtagtcg gatataatcg tgttgaatca ggtaatcctg
     2101 tgcatttagt tcaactttta tgtagaacct tagatggttc aagtattgat gatgatagtg
     2161 aatttggacc tgctacatat aatgcagtta agtcacttca aggaagactt ggattaacaa
     2221 gagatggaat tgttggaaga agtacttggg atgcagctgg aagacgtctt taaaaagaaa
     2281 gctattgcac agataatttt aaattgctga atctgtaaac atttacatgt attctttaaa
     2341 taaaaattta ttttttaagt gataattaaa tttttactaa tagggctatg gctttaaaaa
     2401 tcatagcctt attttgatat cttttccatt tttgcaaaag ctattaaccc tttaagaaaa
     2461 tttcttgttt ttttgaaaaa tttctttctt caaaataaat acttaaatga gctaaaattt
     2521 gagcacaatt tctaattttc tgagtccatt tttaagaaat atatatggtt gttaagtata
     2581 gtgatttgtg caaataatta tccaaagtaa aaatagattt caatttagta acttttctta
     2641 ataatctatt ctaacttccc attgaatttg tagtatatat cgatgtattt atttcttgag
     2701 agtacttaaa atatgttact aattcattcc aattgtttca ccaacctctt aatactattg
     2761 tatattgatt gtactatttt ttctctagtg atagctcact taatgctacc tcttctgtaa
     2821 ttgtagcata tctttcttta aatcttttac agattctttt ctatttttat atgatacata
     2881 atttaaagta tttttaattt gatgcacaac atatctttgt acttaagtat caggaaatat
     2941 cgctttaatt gcattagaaa attcaactag tctatctata gaagctgcta ttaatacatc
     3001 tttaattccc atgtttctag catctataag cacttttagc caatactttg aaatttcatt
     3061 ttctccaatc catattctta aaacctcttt tatgctacat atagctatat agactgtttt
     3121 gtttattatt cgacctcact tctaacttta taatgtatag catctataaa tacaattgaa
     3181 taaattatat taagaggtct aagttaccat ttatgagtta aaggaataac tttatcagta
     3241 gttttagtta ttagtgaatg agataatatc aattccataa gtacttttaa gatgacttaa
     3301 tatattttga gtagacatgc cttttgagta tattccaatc acttgatttt ttattcctaa
     3361 tatatcattt tctatcataa cctaaaagtt cattaagctc cgcttctaac atttaatgga
     3421 ttgtactttc aaataaatct tttagtatat cttgaatatc tgtaattgtt ttggcatcat
     3481 attcttcaat aaattttttt aatattttag tttttgttaa acataaaaag tgaacatctt
     3541 cctttgtata gtagtgatat tttaattatc acaacttttt agaagaatac aaactttatt
     3601 ttaaagacac tgaaaatggt gtttttatac ctttatttgc tatgtataat tttacttata
     3661 acataaactt atataaaaca gatattttaa taacacaaag gtacaattag gagattgtta
     3721 aaatttatta gatattactc tagtttattt aaaaaaaagt attataatat tccttgtgtg
     3781 aaaatatagg aatttttatt aaataatttt aatgtggaaa taacattctg cactattata
     3841 attaaatatg taaaaagcaa aatacttgaa caactatatt ttaaaatcta acttttagta
     3901 ttttaaaaaa ttagaaaatt aataaaaagt acaaaaatcc acattttttc ataaagcgtt
     3961 tgactaaaaa attcacatat tgtagactta aagtagcaag agttttgagg gaggtgtaaa
     4021 tgatgataag aaaaatacca caagcagaac taaaagtaat gaaatttatt tggaaagtag
     4081 atgctacagt gacatcaaaa gatgttattg aagttatgga acaaaaatat ggttggaaac
     4141 aaaccactac actaacactt ttatcgaggt tagtaaaaag aggattctta aatgctcaaa
     4201 aaatagacag atatacacat tacacagtaa tagtaaaaca caaagattat ttaagttttg
     4261 agacaaagga tttcctaagt aatatacatg atgattcttt gaaaagctta atttcagcat
     4321 tacatgatga tgaaaatata ggtaaagaca cgctagattt tttagaaagt tattttgata
     4381 atttaaagaa tgaataatta tatgctatat gtttttgaca atcttataaa gacaactatt
     4441 gtatgtagtt ttggtatttg tctattatta tttttgaaaa gatatttttt taagaggttt
     4501 agtaagagat ttaattatta tatttggttg gtaatagtct ttagaatgtt actttttcta
     4561 tttaattata cagtaatgta tgaagtaaaa gagcccaagg aaaatacagt aggaaataat
     4621 attacacaaa ttgatgtttc tatagataat gattttatgc tgtatttagt ttatttatgg
     4681 ttatttgtaa ctatagccat tgcagtttat acatttatgg aatacacaaa atttaaaaat
     4741 ctcgttgttg atgtatctta tgatattgaa gatcatgatg taaattgtat ctataaaaat
     4801 ttgttaaaag aattagacat aaaaaagaaa attgagttaa gaggttctga tgaattgata
     4861 agtccagcag gtatgggatt atttaaatct tacatattct taccagacta tacttatagt
     4921 aaagatgagt taacttggat tttgaaacat gaattaatgc attttaaaaa caaagatatt
     4981 ttaataaagt ttttagtttt gtctgttaaa ataatgtatt ggtttaatcc acttgtttat
     5041 ataatgagca ataaagtaaa tctagattgt gaattatgtt gtgatgaaag tgttttgact
     5101 gattgctctt taaaagataa gaaagcatat gcgttagcac ttataaagtc aattaaactt
     5161 agtaagaatt ataatagtgg tattttaaca actgaattta ataagacaaa tttggaaaag
     5221 agacttgata gtattattaa gaaaaaaggg aaaagtggaa tattgatgtt attgattttg
     5281 tttacattat tttcagttac atattttaat tttgaaagta ttagttttga taattcaaat
     5341 tatttaagaa atatagggat tttagaaaaa acaggcgcag atcttaagga agaagatgat
     5401 attagcatga gagttgtaaa ttaatagatt tttatagtat tataatttat taacaaatta
     5461 ggagaagttt tgaaattaaa aatctaaaaa aaatcttagt agtcacaggg agggtgactc
     5521 taatatctat tagtttagta tttgcaaata ctaataatga tgtaaatgta tgaaaaagta
     5581 atgaagagca aacaacaaat tcatttacaa taactggtta cgaatttatt tatgaaaatg
     5641 taccacaacc tataaaatct gattatgaaa aaaatgtaaa gaattaaata taaatccatc
     5701 taaagatgat acaatcttta ttaatgaaaa atttatagat tcatataata ttaatataga
     5761 tgattataat attgaaccaa aagcatttat tattagatat aaaaaagatt taaatcgttt
     5821 tgatgctact ggtcctataa gttatagcgt aaatataaat accactgtag taggatatgg
     5881 acatgtagaa aaagggaatc ctgtgcattt agtacaactt ttatgtcaac aaataaatgg
     5941 ttctgatatt gatccagata gtaaatttgg acctgctaca tataatcaag taaaaatact
     6001 tcaaggaaga cttggtgtaa caaaagatgg tgttgttgga aaagccactt ggcaagctgt
     6061 cggagagcgt tattagaaaa gaggttgtcg cactaagaaa tagggcgaca acttttttat
     6121 atagaatata ttaatcagta tcttagagtc tagaaatatt ttacaataaa ttccatataa
     6181 ctctaccatc tatatcctta acattttgac aaccagtcaa aatcatagtc tcacataact
     6241 cattcctaac cttctctata aaaaactcca caccatcaag accaccgcca aaagaagcag
     6301 taacaaaagg tcttcccatc aaaacagcat cagcacccaa tccaagcatc ttaactacat
     6361 caacaccagt cctaactcca ccatcaacaa gtattgttat ctttcccttt acagacttag
     6421 ctatatctgg aagtacctca caagtaccag gagtataatc aagtactctt ccaccatgat
     6481 ttgaaacaat aatggcacta gcaccagact ctacagccat caaagcatca tctacagtca
     6541 taattccttt tataataaaa ggtaatttag tagattcaac caattctcta atctcatcaa
     6601 ttgtcttagg agaaaaagga ttttcttgaa attgattatt aataagtcca caagcatcaa
     6661 ggtctacacc aactgcaaaa gcacctgctt cttcagacaa tctaattttt tctattatct
     6721 ttgaattatt ccaaggcttt ataaatacaa ttccatttcc actattatct ttcaacacat
     6781 ctaaattatc cagtaaaaaa gtatctacat tagtatcgcc aaccatagca taaattccag
     6841 aattagaaca acctctaaca acaggctcta tgtattcttt ttcggaaact ttaccaccca
     6901 tgttcaatag agtacctgat acaggtgcag cgaatatagg agaactcatt tttctaccaa
     6961 ataattctat ggatgtatct ggctttgaaa cattatgtat aactctcata tttatcttta
     7021 ttttttctag actttttcta ttttcaatga aagacgaacc acttccttta ccacccatac
     7081 caggaacatc tccagcacaa gctaatccat tacaaatttt acatacttta catgttccat
     7141 taaagttctc tctagctgat tttagtaagt cattgtaatt catacaaaat ctctcctttt
     7201 tataatatta aaattgagta ttactatatt gaaaatatct taattgtaaa tatagggatg
     7261 ttattatgat gagctaaact agataataca tattagatat ttttta
//
LOCUS       DAMJVX010000170         7289 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMJVX010000170 DAMJVX010000000
VERSION     DAMJVX010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7289
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.176"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            123..1628
                     /locus_tag="PC407_003087"
     CDS             123..1628
                     /locus_tag="PC407_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDF2498497.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            2018..3115
                     /gene="dinB"
                     /locus_tag="PC407_003088"
     CDS             2018..3115
                     /gene="dinB"
                     /locus_tag="PC407_003088"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDF2498498.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGKPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKSSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            3371..4507
                     /locus_tag="PC407_003089"
     CDS             3371..4507
                     /locus_tag="PC407_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498499.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            4500..6047
                     /locus_tag="PC407_003090"
     CDS             4500..6047
                     /locus_tag="PC407_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2498500.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            6381..7247
                     /locus_tag="PC407_003091"
     CDS             6381..7247
                     /locus_tag="PC407_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2498501.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
BASE COUNT         2774 a          796 c         1297 g         2422 t
ORIGIN      
        1 ttaattgtat aatattactt tgagtgaaaa agtattaaca tttttttata agatgtttta
       61 ctaaaaaaat atatataaat atactgttat caacaagaaa tttaatttag tgaggtgtta
      121 agatgaaagt aggatttgac cacaaaaaat atttagaaga gcagtctaaa tacatcttag
      181 aaagagtaaa taattttgac aaactttatt tagagtttgg aggaaaattg atgggagacc
      241 ttcatgcaaa aagagtactt ccaggatttg atgaaaatgc aaagataaaa gtattacaac
      301 atatgaaaga aaaagttgaa gttgtaattt gtgtctatgc aggagatata gaaagaaaca
      361 aaataagagg agactttggt atcacttatg atatggaagt tttaagatta atagatgatt
      421 taagaggtta tgaactagat gtaaatagtg tagtaattac tagatttgaa ggacaacctg
      481 ctacaactgt atttataaat aaattagaac gtagaggaat taaagtttat aagcattatc
      541 caacaaaggg atatccgtca gatgttgaga ctatagttag tgatgaggga tatggagcaa
      601 atgaatatat agaaactaca aagccaatag tagtagtcac agcaccagga cctaatagtg
      661 gaaaacttgg tacttgtcta agtcagttgt atcatgaaaa taaaagaggt aatgtagttg
      721 gatattctaa gtttgagaca ttcccagttt ggaatgtacc tgtaaaacat cctttgaata
      781 tagcttatga agcagctaca gttgatttaa aagatgtgaa tatgatagat tcattccacc
      841 ttgaaacgta tggtgaaatg tcagtaaact acaatagaga tttggaatta tttccagtgc
      901 ttaaaaaaat aatagaaaag ataactggaa aagaatcaat tttccaatct ccaactgata
      961 tgggtgttaa tagagttggc tttggaataa tagatgatga agtaataaga aaagcttcta
     1021 tagaagaaat aataagaaga tactttaaaa ctgcctgtga gtataaaaag ggacaagttg
     1081 ataaaggcgc ttatgataga atgaaaatga ttatggaaga gcttaactta aagccagaag
     1141 atagaaatgt tgtattacct gctagaaatt acagtaataa attaaaagaa agtgccgaca
     1201 agaatgatac ttgtcctgta gttgcattag aattagaaga tggtacaata ttaactggta
     1261 agggttctga tttaatgaat ggaacagcag cagtagtatt aaatgccatt aaacatttag
     1321 ccaatatatc tgatgatatg cacttaatat cacctgtaat attagaacct atcataaact
     1381 taaaaacaaa gactttagct agtagaaatg tttcacttag ttgccaagag gtgttaactg
     1441 cacttagtat ttgtgctgta actaatccaa ctgcacagtt tgctatggaa aagttagcat
     1501 tattaaaagg gtgtcaagct cattctacta ctatattaaa tagagatgat gaacaaacat
     1561 ttagaaagct tggtatagat gtaacttgtg acccagaata tccttcacaa aatctttact
     1621 atagttaaaa ttatataaaa aaatatgggc tgttttaaag taatagtttg agcagtccat
     1681 tacaattata ataaaatcag taggctgtct tgtataagac agtcttttgt ttttaaaaca
     1741 tcccataaat taaatatata tactgttatc cataaggaat tttaaattta gttccttgga
     1801 atacctattg taaagctttt atatagtttt tgcaagtgtt tttaaggatt ttttataaat
     1861 tatattgaag ttaatggatt atagatggtt ttatttatgg aaaatatgaa tatataaaga
     1921 aaataatgaa aaaataccaa aaagaaacat aagtttggta taattaaaaa gtaatataaa
     1981 taaaattaat ctatttatac tttaaaaggt gataataatg aaacattata gaaaaataat
     2041 tcatatagat atggacgctt tttatgcatc tatagaacaa agagataata aaaaattaaa
     2101 aggaaaacct gttattgtcg gtggaaatcc acaaagccga ggtgtggttg caacgtgttc
     2161 ttatgaagct agaaaatttg gtatccattc agctatgcca tcagctgtag cttacaatag
     2221 gtgtccatat gcagtttttg taagacctag atttgatgtt tataagagtg tgtcagagca
     2281 aataagagat atattttata gatacacgga tttagttgaa ccattatctt tagatgaagc
     2341 ttacttagat gtaacaaaaa ataaaaaaaa tattgattca tcaattgaga tagcaaagca
     2401 aataaaaaaa gatatattta gagaagttgg tcttacttct tcagctggtg tgtcatacaa
     2461 taagtttctt gctaagattg catcagactt aagaaagcca aatgggttaa ctgtaataac
     2521 cgaagaaaat gcccaggact ttttagataa attacctgtg aataaatttt ttggtgttgg
     2581 aaaagttact tcaaatactc ttaaaaattt aggtataaaa actggatatg atttaaggtg
     2641 tttaaattta tttgaacttg aaaatatatt caaaaaaaga ggttatgaat tatataaatt
     2701 tgctagagga atagacgata gaccagtgga accaaatagg gttagaaaat ctgttggggc
     2761 tgagactaca ttaagtcata atttggatat tgatgaagaa gaaacacgca acattcttga
     2821 tgaactttgt gaagaagtat gtcatagatt aaaaagctct gagaagtttg gaaaaacatt
     2881 aacattaaaa attaaatatg aggactttac aaagataact agaagcctta gtttagaaca
     2941 ctatattgac gaatataatg atataagaag tggtgtagat aatttgttga gaaatgttga
     3001 ggttaatgga aaacaaatta ggctattggg agttacaata tcaaacttaa gtgataaaaa
     3061 agaaacttat aaagatatta cattatttga atacatggat agtattcaaa tgtgagtttt
     3121 gtttaaatga aataacacta gctttaatac tatataattg aggctagtgt tattttatga
     3181 atatttttat actttttgtt ttttgacatt atatactata tataaatact ttatatttgt
     3241 agcaatattc agacaaatag tgacctaaaa atcgtacttt gtttacataa ataaaatagt
     3301 atgatataat tataaaaaca acttataata ttggagaagg ttgcttgttc gaaaaagggg
     3361 atgataaatt atgatagaga taagaaatgt aacaaaaaaa ataggaaata atgttatatt
     3421 agatgatatt tctctagttg tagaaacagg tacattggta gttttgattg gttcaagtgg
     3481 ttgtggaaag actactactt tgaagcttat aaacaaatta ataaaaccta catctggtga
     3541 gatatatatt aatggcaaac caatttctca ggaaaatgaa atagagctta gaagaaaaat
     3601 aggatatgtt atacagaaca caggcctatt ccctcatctt acaataaaag aaaatataga
     3661 gctaattcca cgcctaaaaa aggaaaaatc cgttgaagaa attgaaaaaa gaacgttgca
     3721 actacttgag atggtaggac ttgatagtga tgaatttttg aataagtatc cttcagaact
     3781 tagtggtgga cagcaacaaa gaattggtgt tgcaagagcc attgctactg atgctgaaat
     3841 aatattgatg gatgagcctt ttagtgcttt agacccaata acgagaacat ctttacaaga
     3901 acaattattt tctcttcaag atgaattgaa aaaaactata atatttgtaa cgcatgatat
     3961 ggatgaggct ttaaagatag ctgataaaat ttgtattatg aaggatggaa gaatagcaca
     4021 atatgatact cccgaaaata tacttagaaa gcctgcaaat gactttgtaa aagattttat
     4081 cggagaagat agagtttggg ataatccaga atatataaaa gcaagagata ttatgataaa
     4141 aaatcctatt gctgttaatt ccactagaac tgtaactcaa ggaatagaaa ttatgagaac
     4201 gagtaaggtt gatagcttac taattataga tagagcaaaa acattaaaag gtattgttac
     4261 tgtaaaagat atgaaagata ttgatgataa gtcaatatta cttgctgata ttatgtcgag
     4321 tgagcctttg catgtaaatg aaggtgataa tttagtagaa atactaaatg taatgaatcg
     4381 taattcggtt ggatatattc cggttatttc tgatgaaaat aaactggtag ggcttattac
     4441 tagaagtagt ttactttctg ttttaagtga acaattctta gaaatggagg tgagtgttct
     4501 tggctaattt ttttaacttt atactattac aaaaagataa gattatagag cttttaatac
     4561 aacatatgag tctaactgtt acatctattt taatagctat aatagttggt gttcctcttg
     4621 gaataattat ttcaagaatc tcatctctta gaaagtttgt acttggattt gtaaacttag
     4681 tccaagcagt tccgtctatg gcacttttgg gtcttcttgt acctatactt ggcataggaa
     4741 gtaaaccagc catatttatg gtagtggttt attcattact accaattgta aaaaatacgt
     4801 atataggtat aacaagtatt gacccagtag tattagaatc agctaaaggt attggtctta
     4861 caagaaatca gacattattt aaaatacaat tccctttagc acttcctata ataatgggtg
     4921 gagttagaat atctgcagtt acagctgttg gtttgatgac tcttgcagca tttataggtg
     4981 ctggtggttt aggatattta gtattttcag gggttcaaac tgtaaataat aatatgattt
     5041 tagcaggggc aatcccatca tgtataattg cactgttagt tgattattta tttagtaaaa
     5101 ttgaagttgc agttactcca aaaggattaa atccaaaagc acctaagaaa aattatatag
     5161 cacttaaagt aatatctgta gtggttgttg tttcaatgtt gttcgttgtt tttagttcta
     5221 gtttttcaag taaaaaagat acaataacta ttggttctaa agattataca gaacaactta
     5281 tacttggtaa tgtttatgct gaattagtag aaaaaaatac gaatcttaaa gttaagaaga
     5341 atttaaatct aggtggttca tctgttgcgt ttaatgcaat taaatctggt gagttagaca
     5401 tgtatgttga ctatacaggt acacttttgg taaatgtaat gaaacatgct ccgattaaag
     5461 atgctgatga agcatataat gttgtaaaag atactatgga aaaagaaaat cagttaactt
     5521 tacttgaccc tcttggattt aataatactt acacattagc tatgatgcct gaaactgctg
     5581 aaaagtatgg aataaatact atttctgatt tgactaaata tggtaaagaa tttacattct
     5641 caccaacttt ggagtttgaa aatagagaag atggtcttgt agggctttct agagattatg
     5701 gtttaaaatt caaagatgta aaagcaatga atggaagttt aagatataca gctttagata
     5761 ataatgaatc tcaggttata gatgcatttt taactgatgg tcttattaaa aagtttaatc
     5821 ttaagatatt agaggacgat aaaaatttct ttgtaccata ttatgcagca ccattagtaa
     5881 gagaagatac tttgaagaaa tatcctgagt tagaaaaagt tatgaatatg ctttcaggaa
     5941 aaataaatga agaaactatg agagaactta actatcaagt ggatgaactt ggtaaatcac
     6001 cagaagaagt tgctcattca ttcttagtta aagaaggctt agtataaaaa attaatagtt
     6061 tatatcaaaa atttaaggga gtgtctcaaa atgaactttt tagttcatga ggcactcttt
     6121 tttatatgaa atcaaatata ttttatcatt tcaacaataa aaaagaagct tatctttatt
     6181 ttgagacagt ctcttatttt tatgtatacc aaaaccatac ctacgatttt tatttataat
     6241 taagtttcaa atagaatgaa caagtacaac caattttgaa tataaatata gtaaatgtgc
     6301 ttatatacga ttttattatc tgggtgattg atattgatag atagctttct ttattgaatc
     6361 gaagggaggg ggaatttgtt atggataact ataagaaagt gaagcaagta ttgtggataa
     6421 ttttatttgc taattttgca gtagcattat taaaaatcat tattggtaat caaataaaaa
     6481 gctatagtat gacagcagac ggatttcatt ctttgtcaga tggagcatct aatatagtgg
     6541 ggcttatagg tattttcttt gcatctaaac caaaagataa aaatcatcct tatggacata
     6601 aaaagtttga aattataacc agtttattta taagtggtat gttgtttgtt atagcaatta
     6661 agataattct tagtgcagtg ctaagaatag ctaatccagt tgtacctgct ataactatag
     6721 aaagtttaat tgctttaatt attacacttt ttataaatat ttttgtatgc atgtatgaat
     6781 atagaattgg gactaaacta aatagttatg ttttaatttc ggattcattg catacaagaa
     6841 gtgatatatt tgtgtctcta ggtgtattgg ttactttagt tggtgttaag ttaggatttc
     6901 cagtaataat agaatctata gttcctatta ttatttctgc attcataatt tattcggctt
     6961 atggaatatt taggccatct attggaattt tggtcgatag agtggctgta gatgaggatt
     7021 atataaaaga aattgtgttt gaatttaatg aggtaaggga tgttcacaat atcagaagta
     7081 gaggtagcaa aagttctatt tatattgaca tgcatgtaat ggtagaccca tttataagcg
     7141 tagagcaatc tcatgattta acacataaaa ttgagaaaca aatacaagaa gaaattaatg
     7201 aaaatgctca agtaatagtc catattgaac ctttttatag tttttaactt agctataaaa
     7261 tacaagttat aaaaaaatat aatttataa
//
LOCUS       DAMJVX010000171         7263 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMJVX010000171 DAMJVX010000000
VERSION     DAMJVX010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.177"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            136..1029
                     /locus_tag="PC407_003092"
     CDS             136..1029
                     /locus_tag="PC407_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2498502.1"
                     /translation="MQLYNTPFNTNKHLIYFFTSTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            1207..2031
                     /locus_tag="PC407_003093"
     CDS             1207..2031
                     /locus_tag="PC407_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2498503.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKHEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREVVPDYQSEGILWEKLSKFIKEEHIE
                     VSRQPNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            2130..2738
                     /locus_tag="PC407_003094"
     CDS             2130..2738
                     /locus_tag="PC407_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2498504.1"
                     /translation="MEKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            2842..3018
                     /locus_tag="PC407_003095"
     CDS             2842..3018
                     /locus_tag="PC407_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498505.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            4314..5066
                     /locus_tag="PC407_003096"
     CDS             4314..5066
                     /locus_tag="PC407_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498506.1"
                     /translation="MLKVNQLCKSYKTGNKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            5056..7167
                     /locus_tag="PC407_003097"
     CDS             5056..7167
                     /locus_tag="PC407_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2498507.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYI
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVAGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIERVYMKKSSYDMFFNSK
                     DFSKLIDKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         2702 a          960 c          961 g         2640 t
ORIGIN      
        1 attaatcaaa atataaaaaa taggatatta ttataggcaa taactaacat caaacgataa
       61 cttaatacat ataaaaataa ataaacaatt aataaaaatt aaaacttcat tataaaacgg
      121 aaggatagtg aaaaaatgca actatataac acccccttta atacaaacaa acacttaatc
      181 tatttcttta catctacgct agtattgaac tggatattca gtcttacacc tgcgttgctc
      241 ggaattacaa atacaatact tggagaaatc attttttatc ttgcatatac atcaccagct
      301 attgtaggtc tattttttat ttctacaatc tatccacaaa gtgcaaaaca agattttatt
      361 tatagatacc taagctttaa agaaattaga ataaaatgga tactattaac aatattattt
      421 tttgctttaa cttttattac ttcgttaata atcggaaatt actttaatgt aaaaacttct
      481 gatatagatt ggatacacat agcagtgttt agaccttcta aaatagctta tatgctatta
      541 gcaagtttta tattgagtat acttagtcaa gaagctggat ggagaggata cgccattgac
      601 aaattattag ttagatttgg atttataggc tcttcaatta ttttagggct tgtttgtgga
      661 atctggtatc tagggtcgta ttttacacct aatcaaatac catataattt ggcacaatac
      721 tccctatttg atgctttttt atttatacca aacattgtac ttttaaactt tataattaat
      781 tttgtatata tcaatacaag tagaagcatt ttatcagctg ggttagttca tatgatgtac
      841 tattttttca acattcaatt acttctacac taccctatta aattatgtgt aatagctcaa
      901 tatgtacaaa taatatttgg tttaatattt cttgtatata tgatttcttc taataaattt
      961 aaacagaagg taaattctga aattgaacag ataaagtcgg atgattttga atttgagtta
     1021 gactggtaat tttaatatga attataaatt aagataatct atgatttgat acgattacta
     1081 cttaaatata atttactctc tataatttat tgcaacatct ggaaaataat tgtatttcca
     1141 caatcgcttg aagtatataa aaacaaaata tatactaatt ataagtgaaa aggaggtcat
     1201 attttcatgt ttaaaatagg agaggtttca aaactaacac aaatttctat tcgtatgttg
     1261 cgttattatg atgaacttgg tattttaaag cctgcagaaa tagacaaata tacaggtcat
     1321 agattatact cagttgaaca gatttcaata ttacaaagaa ttgtattatt gagagattca
     1381 aaatttagcg ttgcagaaat agcaaatatc gtacataatt ggaatgatga atttgtaatt
     1441 aaagaattga atagaaaaaa gaatgaaata caaaaggaaa taaagcacga acaacaaaga
     1501 ataaataaaa ttgataaatt tatagaagct ataaactgtg ataaagatga aattcattac
     1561 aatgttgtct ttaaaaagat tccaagttat aaaatcatat cacttagaga ggtagtccca
     1621 gactaccaat ctgaaggtat actctgggaa aagttatcta aatttattaa agaagaacat
     1681 atagaagtat cacgtcaacc caacaataat atagcttttt accatgatga agaagtcaaa
     1741 gataatggtg tagatattga agttggaatg gttgtaaaga agattggaaa gaacaaatct
     1801 ggatttatat atcgtgagac agaagaaatt gatatgatgg catgtactat ggtttatggt
     1861 ccatatgaaa atatagctgg tgcatatgaa tcgttctgtt attggttaga taaaaatagt
     1921 gattatcaaa tatcaggtat aaatagacaa atcggtcata agggtgagca taatgaaata
     1981 aatcctgaaa attatcttac agaaattcaa atccccctta taaaagtcta atcgattagt
     2041 acttaaatac ttaagtacta atcacccatt gacatcccaa aattttcatg atagtatcta
     2101 aacgtgtatt aaacatgttt aattttttta tggagaaact tttaagggga aaaattatga
     2161 aagcagattc aacaaaggaa caattaatta atgccacaat ccagttacta tcttcacata
     2221 gagatgtttc aagcattacg gctagagaaa ttgtagctaa agcaaatgtc aatttagcta
     2281 tgattaatta ttattttaaa tctaaagagg agttaattaa tatttctatt aaaaaatttt
     2341 taaagcgtca tatggatgaa tataaggcta cacatgatac taaaaattta gcacctaaac
     2401 agcaattaag ggaaatacta attgcatttt ttgattttat aggtgaacat agccaattta
     2461 taaagatttc tgtaccctat atattgatac aagaagaaat tgtttatcca tttgagatac
     2521 tacctttaat taaggctcac tatgaaaatc agaataacaa aaaaactgaa gctgaataca
     2581 gaactattgc gtatcaaata acttcattta ttcagttgct ttttcttcgt tctaatgctt
     2641 ttttcaagta tagtggtatt gatattctta atgttgaaca gagaaatggt ttaatagatt
     2701 ttcaattaga tttattattg gtagatatta caaaataata ctatattaat tttttatgta
     2761 cattttttta ttttatggta acgtttgaaa atattctcat tgactaaaaa tacttgcatt
     2821 aaataagggg gaggaatttt aatgtttttt acatttatag gagtaatatc agcaatttta
     2881 ttgttgtgta caattatatg tggtttatgg ataaaactta ataatcgtaa taatgactct
     2941 aattctcact ttactttttc aatactttca attgcttttt cactgttaat tgtggtggcc
     3001 tttttagtaa gaaattaatg tctttgttat gctggggttt attattatag atttttatta
     3061 ttgcatgatt tttactatat tatagtattt ttattatatt acagggtctt atttattata
     3121 gtgttttgtt attacagcat tttattatat tacagagtcc tatttattat aggattttta
     3181 ttatattgta aaatcttatt tattatagga ttttgttata ttacagactt ttattatatt
     3241 acaatctctt atttattgca gtgttttgtt attacagtat ttctattata ttgcaaactc
     3301 ttatttatta tggtgttttg ttattgcaac atttctatta tattataaag tcttatttat
     3361 tacagttctt tgttatacta cagactttta ttattacagc atttctatta caatctctta
     3421 tttattacag ttctttgcta tactacagac ctttattatt gcaacatttc tattacattg
     3481 caaagtctta tttattacag ttctttatta tactacagac ttttattatt acaacatttc
     3541 tattatatta caatctctta tttattacag ttctttatta tactacagac ttttattatt
     3601 acagcatttc tattacattg caaagtctta tttattacag ttctttatta tactacaaac
     3661 ctttattatt acaacatttt attattttaa cgtctttact ataatatagt aatattttaa
     3721 tatctcaaaa tcatatcaat cacttttcag catagtctaa aatacttcca ctttctaaaa
     3781 ttatctatca tacctatact aataatactt tttaaccttt ttattataca tttcaattgt
     3841 ttttaacgac ctatttattt ccaaaactcc aatatatttt ctcaattaca ttgtgttaat
     3901 aacccctaaa tcctttcaaa ttatttacaa tacattccat taaataatct tactgctcta
     3961 taattctttt tttcttttta accctactat acattattta catcattttt ttcaatacaa
     4021 cattcgttat tctataacct ttttactttt actatatacc ttttttctaa ctacaccttc
     4081 aatgttccac atcatattac tttagcacaa caattaatac ctaaaaataa ccctctaaat
     4141 cctccaataa tataatcaat ttaactataa taataacctc tcacactatc caaacaatat
     4201 attttcaaac attacaaaaa aacatccaac tattacaata ttgctcatat ccctcctccc
     4261 ctttcaaatg ctatcattga cttacaagat aaatgaaagt gaggatgata tatatgctta
     4321 aagtaaacca attatgtaaa tcatataaaa caggcaataa aacatatcct gtattaaaaa
     4381 atatttcttt tgaaatcaac aaaggagagt ttgtcgcaat catgggttca tcaggaagtg
     4441 gaaaaactac tcttttaaat tgtatttcat gttttatccc aattgataat ggggaaataa
     4501 ttcttggaaa taataatata agaaatttaa aagaaaagga actttcagaa gttagaaacc
     4561 aacaactagg atttgtattc caagatttca tgctatttga tggtcttagt gtctttgaaa
     4621 atatatgtgt tccacaaatt atcaaaggta gtcaagtctt acaaatggaa aaccgtgcaa
     4681 ttaatctatg cgagctattt ggtattgaac atatcagaaa aaaataccct gccgaaatat
     4741 ctggtggaga aaaacagcgt acagcagtag caagagcact catgaacaac ccagatttaa
     4801 tattagcaga tgaaccaact ggtaaccttg atagtaagtc ttgtcgtaca gtcattgaat
     4861 catttttaaa agcaaagcaa gaattagatg ctactatact catggttaca cacgatagct
     4921 ttgcagcaag cttctgcgac cgagttattg tgcttaaaga tggaataatt tataaagaag
     4981 ttgtaaaatc aaattcaaga cataaattcc tagatgaatt actagaagtt ttaaaagaaa
     5041 tgggaggcgc tcaagatgac aattaataaa ttatacagca aacttcgagc ctataattgg
     5101 aagaactata caactttatt cttttgtata atactttctg tggtattagt aacttcatat
     5161 gctctaattt attttagccc aacaatacaa aacatacttc caactggtgg agatagcaga
     5221 aaacaggcta caatgatatt ctcagttgct atattaggat gcgctgtctt tactacatac
     5281 gcttcttccc tctttttaaa atttaaaagc aaggaaattg gtattttcat ggctctagga
     5341 acagataaga agaaattaaa aaaacttcta ttttcagaga tattgttaat ttcaatagta
     5401 tgctgttttt taggtcttat attaagtatt cctgtatctt ttggtatatg gaaaatattt
     5461 caattagtga ttattgatac cagagaaatg acatatcatt ttggaactca aggattcata
     5521 tatggattta tatttagtgt atttgtgaca ttatgcatat ttatactttc tgtaaaattt
     5581 attaatcgta caaatataat tgatatatta aatgaccaga gaatatctga accaatacag
     5641 gatgtaaagg actggtatgg tatattaggt ataattttta ttatactagg tatttttcta
     5701 ggatatatcg taccttcact aactataaac aaattaaatt atagactacc atctgtatgg
     5761 agctttactt atctactctc tcttattgga atatatatga ttatgacata catagttgta
     5821 catgctaaaa gaggaaaaca tccagaaaaa tactataaaa acatcatatc aactagcatg
     5881 atgagattta tgggaagaca aacagtaaaa aacatgtgtg ttatttcatt tttgattgca
     5941 ggagcattat ttgctgtatt ctatgttcct aatatggttg caggtatcta tgatactctt
     6001 aaaaataacc ctatagatta tacttttacc tacaatcaaa gagataaaca ggtatcaaaa
     6061 aaagatattg aacaattagc agataaacac aatataaaaa ttcaaaacta tatggaaatt
     6121 ccttctatag aattaattgt tgatggtgta gaatcaatat atcataaaga tggaactatc
     6181 gaaagggtat atatgaaaaa aagctcctat gatatgttct ttaattctaa agatttttcc
     6241 aagctaattg acaaaccaat aactataaaa aatggtgaat atcttgctgt tgtaaataaa
     6301 ggaaatgaaa atcaagatta taaatatacc aaatttatat caagcccagt cacagaaaaa
     6361 gaaatcccct ttagatatgc tggaaaaata atatttaatg gttctttttt tcaaaataga
     6421 agtatgaacg cctatgtcct taatgatgag gattataaat catttactaa agatttatcc
     6481 ataaaaaatt acaataattc tatactattt aatgtagata aagataccta tgcttttgcc
     6541 aatgacttaa agaaagaaat tataaaaaga acatctaagg aaatgggcat gttcagtggt
     6601 tatgatgaat acgtaaaaaa acgttatgaa tcaactggaa gaaaatattt tatggatgaa
     6661 gaatatccaa gtggtgaagg tcatttaaaa ctagacccaa acaacaatca gttgtatctt
     6721 aattggaaat actatcctaa ctttaaaatt ttacaaagcc aagatatgat aaaaaacatg
     6781 gcagtcttct taatgttatt catatatgtg gcaatcatat gttttacagc agtaggaatc
     6841 attgcatata caaggggtgt tactatagct ataaatcaca agcaggtctt tatggattta
     6901 aatcgattag gagcaaacaa tgattatata aagttctgta ttagaaatca attaaagaaa
     6961 gtattttcat accctcttat tgcaggaagt tttttaattt atctgtttaa ttttatgatt
     7021 atgttaaata atgatggtag aatcgtcaat agtgaggtca atgctctttt gataaactta
     7081 ggttttatag ggatttgtgg agtttatatg taccttatat atctattaac ttataagaaa
     7141 ttcaaaaaaa ttattggtat tttataataa ggaaggttgt tagattttta aagtaataaa
     7201 atcattactt tttataataa gaaaagcatt agcattttaa attaaaaaag gttattagat
     7261 ttt
//
LOCUS       DAMJVX010000172         7168 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.178, whole genome shotgun sequence.
ACCESSION   DAMJVX010000172 DAMJVX010000000
VERSION     DAMJVX010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7168
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.178"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(133..972)
                     /locus_tag="PC407_003098"
     CDS             complement(133..972)
                     /locus_tag="PC407_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2498508.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(996..1754)
                     /locus_tag="PC407_003099"
     CDS             complement(996..1754)
                     /locus_tag="PC407_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2498509.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNENVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1801..2274)
                     /locus_tag="PC407_003100"
     CDS             complement(1801..2274)
                     /locus_tag="PC407_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2498510.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDEHLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2303..2719)
                     /locus_tag="PC407_003101"
     CDS             complement(2303..2719)
                     /locus_tag="PC407_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2498511.1"
                     /translation="MIHMTGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRRLEDRIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2766..3818)
                     /locus_tag="PC407_003102"
     CDS             complement(2766..3818)
                     /locus_tag="PC407_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2498512.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGTIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPMTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4214..4894
                     /locus_tag="PC407_003103"
     CDS             4214..4894
                     /locus_tag="PC407_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2498513.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5027..5347)
                     /gene="sugE"
                     /locus_tag="PC407_003104"
     CDS             complement(5027..5347)
                     /gene="sugE"
                     /locus_tag="PC407_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDF2498514.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5729..6556
                     /locus_tag="PC407_003105"
     CDS             5729..6556
                     /locus_tag="PC407_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2498515.1"
                     /translation="MNFYKIAVCQMMTTENKIENINHAVDMVTEAAINGAKIIVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEYGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGVEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
BASE COUNT         2424 a         1302 c          837 g         2605 t
ORIGIN      
        1 atataagttt aaaatttaat ctattataaa attaccctaa ttgctaaact ttttaatata
       61 aattttccaa catcttacaa atataatcaa tagatataga atcctaattt acacaccaac
      121 atatatattt atttaaaata gtcccaaaat acctgtaata gctcccacta ctatcagtat
      181 aaacattatt tttattggag aaaggccttt ttttaaagca aagagtacta ataaagtaag
      241 taccaaagga attaatcctg gaaatatctt atcaagtaaa tccgtttgaa gactaaattt
      301 aaaatcacca ctagtataac ttattggtgt agataaatta ataaacctac ctacaagtcc
      361 ccccatgact aaagtaccta atatagaagc cccttctata accttattta ctattccacc
      421 ttgaagtata ctttcaacag cttttttccc atacttatat ccatttgtcc acattccaaa
      481 agaaattgaa gtcattatta tatattgagc tattacaaat aatataggac ctgcaattga
      541 tgctcctccc tcagttaaac caatacaaac agctaacact ataggagtta taactccttg
      601 agttaaagta tcaccaattc ctgctagtgg acccatcaag cctgttttga tagcattcat
      661 accatctcca cttatttcat ctgctccatt agctttttct tcttccatag ctattgttat
      721 tccatgtata acacatccac atataggttc tgtgttaaaa aatgctaaat gagtctccac
      781 ttcttgtttt aactcttcct tatctggata taatttttta agtactggca acattgaatg
      841 tgagaaagct gttgattgca atctttcata attgtaatta gattgcgcaa agaaaaacca
      901 tcttaaccat gattttatta catctttttt gcttaacgtt ttcttagata cttcattttt
      961 tatatctgac ataatatttt ccccctcaca tgtatttaag atgtagcact atcttttccg
     1021 tacttaattg aagtaattgt aagagcaagt attactccaa taacagatat tactattata
     1081 tctactttaa aataaacagc cattagtatt cctaatatga aaaagggcat agtaaacttg
     1141 tttccaatca attttaaatt catagcaatc ccaagtgctg gcaacatagc cccaattaca
     1201 ttaaatacat gtaacacatt ctcattcatt acttccaaca taccatctat cgcatttgga
     1261 ccaaaataaa caactaataa tgctggtata aaactcattg caaataacag tatctgtggt
     1321 ggtactatat ttataaatgc tactctatca acattccctt tttgaacctc tctatcagcc
     1381 caatgtgcaa aaaaagaatt tactgtcatt tttccaatcc aaagtacagt tgcaacagtt
     1441 cccaatggta cagctattgc cattgcagta cttacatcta gcttagatgc tatagctaaa
     1501 gcagtaccta catatcctgc aaaagctggg tcccccatct gagctccccc tgctgatata
     1561 aaacctagat atatcatatt tatacctgct cctataattg ctccttgaac agggtctcct
     1621 aatataattc ccgttacaaa tccagccaat agaggtctat tccatgtaaa aaatccaaca
     1681 taggtcaacc acggactcca agaaagataa taaaaaattg ctattaatac tgcttgaatt
     1741 agtgaaatgt gcatattctt cctccctatt ttcttttaaa gatttacaag atagtctttt
     1801 ttataataat gttcctacat caattttctt tgcatctggt actatttgta taaagcattt
     1861 aacatctttt gatattagtt ctctaaatat ttctttttct tcatctgaca cagatatatt
     1921 tttataaaaa ctttttctac caagccctgc tcccatacct ccaacattca attcctttaa
     1981 atctacccca ctatttacta aacctaagac agttttaggg tatttgacca atactattaa
     2041 atgttcatct ccattatgtg attttaaaac ttcagcacct gattctacat catgtacttc
     2101 aaccttaaca gttgatggag ctgccatttg taaaacttga actataaatg ggtcctttac
     2161 tactaaatca tccacgatta ctatcctttt tgccgatgta atcttacacc aagcagtaat
     2221 aacttgccca tgtattaatc tatcatcaat tcttgttaat gctatattac tcataattat
     2281 tcctccagat ttttaattta ttttacaact ttaaaacagt tttaacatct ttgattcctg
     2341 atatcccaac ttcctcacat aaattcttca agtcttttaa cttcatagta tctctgttta
     2401 aaaatagctc taaaagcata ggtaaattta cacctgtcaa acattctaac tcacatacct
     2461 tagcaaattt atttattata atagcagatg tattaaatgg agtcccgcca taaaaatcaa
     2521 gcaatactaa aaccccttca tccttatatt tctcaattct atcttctaac cttctactaa
     2581 actcatctat gctgtcacca tggtttaatc ctaaagtttc tacattttcc tgtttgccta
     2641 ctattaattc agcactttct aacattgctt tagccatatt tccatgtgtg gctataatta
     2701 tccctgtcat atgaatcata cctttcttaa ccttgtttat atggattatt atttagtact
     2761 ctaatctaat tatctatatt aatcattatc ttaccaaaga aaatatccct tttgtatact
     2821 ttttcaaaat aagctggtac ttcttctaaa tttattctat gtgttactaa tttttcaaca
     2881 ttcatatcac ccttttgcat atgatacaat ccagcactcc attcttttcc tgggaatgga
     2941 aaagagtttc caaaccaagt tcccttgaca gttaattcac ttcttactat cttttcaaat
     3001 tgttctctag tcaaagctac atccccatat ggtacacctg catacaatac tgtccctccc
     3061 tttttagcta acaacaatac ttgcccacat gtcattggag tcccagccga ttcaataaca
     3121 atatctgcac catctccatc agttaacctt tttatttctt ctacaatatt cttttctttt
     3181 gcatttatac aaatatctgc ccctaactct ttcgccaagt ctaacttctc atcaaataca
     3241 tctacagcta ttatttttgt agagccaaat atctttgccc attgtattga aaataatcct
     3301 attgtacctg taccaagtac tacaacagta tctccaactt tagcttctga tctaaaaaga
     3361 ccatgccctg ctatacatac tggttctagc gcagcggcag tctcataact tatctcatct
     3421 ggtattttta ttaaattcct ctctttcacc tttgtatact ctgcaaaaca acctccaagt
     3481 tctttatttc ctattattgc tacattatta catcttgaat acaatccttt tttacattca
     3541 tcacattcaa aacatggcat agctggacaa acagcaactc tatctcctat tttaaaactc
     3601 cttacttctt taccaacctg tgccacttct ccagaaaatt catgccctaa aatttctcca
     3661 accatatgtg gacctgtctt tgaatatttt gatatatcag aaccacatat tccagctact
     3721 tttactttaa ttattacatc atctttttct aatatttttg gtacatccac atcctccact
     3781 cttgtgtctc ttataccata gaatctaact gacttcacta ttaaccaccc ctacttttta
     3841 tttgagaata tatccctatt gtttatatgt taaatatcta aagcaacttt catgccaata
     3901 tatgtaagca ttgcattcat gcattttata aaattatcat taaatgtgtg tcaaagtgtg
     3961 tcaaaagttt cttatattaa aacttatgtg tgtcaatata aacgacacta acattttata
     4021 ttagttctct tattataagt tttataaaat gtactttttt tgttattttt ataaaattgt
     4081 tcggaaattt cccttctatt tgtgatataa tattaaatat aaggtatcaa tataaatttt
     4141 catgcgaata tcagaacttt tttgacacta atgccttatt caatataata atttaacttt
     4201 ttggaggttg tttatgaaaa aagtgtatca aggaaagact aaagatgttt ttgaattaga
     4261 taatggaaat tacttactta aatttaaaga cgatgtaact ggtaaagatg gagtatttga
     4321 cccaggcgaa aatagtgtag gtctaagtat agatggaatt ggtagagcta atttagagac
     4381 atctgttaag ttctttgaaa ttctaaacaa tgctggaatt aaaactcatt atgtaagtgc
     4441 taatttagaa gaagcaacta tggaagttct accagcaaaa gtttttggta atggattaga
     4501 agttatttgt cgttatagag ctgttggtag tttcttacgt cgttatggtt catgtgttga
     4561 agaaggtgct aaactagatt gctatgtaga agcaacttta aaagatgatg accgttgtga
     4621 ccctcttata acatctgaag gtttagaagc tttaaacatt atgactcaag ctcaatttga
     4681 ttcaatgaaa agcatgactc aaaaaatttc taatatagtt cgtgatacta tagctgaaaa
     4741 aggtcttgaa ctttatgata tcaaatttga atttggattc tacaatgatg aagtaatctt
     4801 aatagatgaa atagcatctg gaaatatgcg tgtttataaa gatggtgtaa tagttgaccc
     4861 tatggattta actgctcttt tattagataa atagtatata catataattt tcaaatataa
     4921 taaacttata aataagataa gctatgaagt aaagactttt taaatgttga ttatttttaa
     4981 acattttctc gcctttaatt tcatagctta tttaaattaa tatactctaa ttagtagata
     5041 atagctttaa ccctacaatt cccaccacaa taaagcctat acatgcaatt cttgatatac
     5101 taataggctc atttaagata gccatgccta caatagctgt accgacggta ccaattccag
     5161 tccatattgc atatgaagtt ccaagtggaa gatgttttag tgctaatgac aaaaaataaa
     5221 aacttacaat cattccaaca acagtaagta tacttggagt taatttagaa aatccatgtg
     5281 aattttttag ttgaaaagcc caaaacactt caaacaaacc tgcaacaaac aaaaataacc
     5341 atttcataaa ctcatccctc ttttcaaatg tatctcttat ttttccaata aaataacctg
     5401 agaaatatta taaaatatct cccaggtttt tgtccttccg tgttcacaac aaaaactatg
     5461 ttgtgggttt tatctcggac caggccaatc attaataatt gcaaaaccct ataaaactct
     5521 attaattatt ctattttatc tataataata ctatcaacat atataaaaaa agtcaatata
     5581 tgggttccat ctataataaa aataactttt atataccttt taatattctt attcacttct
     5641 ctccataccc atttaggtca atctaagaat atctaatttt tatttaatat gttataataa
     5701 cataaaaata ttgaaaggag taatttctat gaatttctat aaaattgcag tatgtcagat
     5761 gatgaccaca gaaaataaaa ttgaaaatat caatcatgct gtagatatgg taacagaagc
     5821 tgccatcaat ggagctaaaa ttatagtttt gccagaaatg tttaactgcc catatgaaaa
     5881 taaatatttt cctaaatttg ctgaagaata tccaggtgaa acaacaacga tattgagtaa
     5941 attagctgag aaacatggaa tttatctagt ttctggtagt attcctgagt tagaatatgg
     6001 aaaaatctat aatacttgct atgtatttga taaaaatgga gctctaattg gaaaacatcg
     6061 taaaatgcac ttatttgaca ttgaagttac aggaaaagtt agttttaaag aatcagatac
     6121 tcttacagct ggaaatgatg tcactgtaat agataccgaa tatggaaaag ttggtatagc
     6181 tatttgctat gatattcgtt tccctgagtt atcacgttta atggctctta aaggtgttga
     6241 aatcgttatc ttacctgctg catttaatat gactactggt cccgcccatt gggagttatc
     6301 tataagaatg cgtgctttag ataatcagat attctatgtt ggtgctgcac ctgctagaaa
     6361 tatgaatgct tcttatatag cttttggaaa ttcaagaatt tcagaccctt ggggaagaat
     6421 aattgctcaa gctgatgaaa aagaatgtat tatttatgct gacattgata gagatttaat
     6481 tcctgacata agacaacagt taccacttct taagcacaga agaactgatt tatatgaact
     6541 taatactttg aaataaaaca gaacataacc ccataaatca taaattaagt gttaaaaaaa
     6601 tttatggggt tctcattaat ttgatttaaa attaatttaa taaaaataat gctaaaatta
     6661 atttaattaa ataatactaa taattactaa tgattatgct aattgtcagc atttactaat
     6721 ttaatcacta ttagttattt cttttattaa atatctttac tttttttact tattatgtct
     6781 tctagcttta catgtggatt atggtatttc tttctatttt tagtcacatc tttctattga
     6841 attgcttgct taaaacacca atgcacacat gccagataat gttcacaatt atgtaaaaat
     6901 attgctctac cttcttcaat gttgatatta tctctcctag ttaatattat ctttcctaaa
     6961 atttctttta attaaaaaac aacattagtt gatatatcaa gtatatttta agcaactgaa
     7021 aaaataataa taattgatat atcaactaat taatgtagat taattcattt ttataattta
     7081 tatacttttt aattaatcta cctttttaat taaaatcctt cctttctatt tatccatttt
     7141 caacttaaat tataagattt catcagct
//
LOCUS       DAMJVX010000173         7144 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMJVX010000173 DAMJVX010000000
VERSION     DAMJVX010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7144
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.179"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            135..1916
                     /locus_tag="PC407_003106"
     CDS             135..1916
                     /locus_tag="PC407_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDF2498516.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(1998..2528)
                     /gene="lepB"
                     /locus_tag="PC407_003107"
     CDS             complement(1998..2528)
                     /gene="lepB"
                     /locus_tag="PC407_003107"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2498517.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSVVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEILG
                     KVMVRLLPLDNIGKVD"
     gene            complement(2568..3392)
                     /locus_tag="PC407_003108"
     CDS             complement(2568..3392)
                     /locus_tag="PC407_003108"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2498518.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILINEFIKLNF
                     SNTFINTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRVFFNSTVVAIALIVYGIIMIGLEKRNKRPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            3649..4557
                     /locus_tag="PC407_003109"
     CDS             3649..4557
                     /locus_tag="PC407_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2498519.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            4701..6146
                     /locus_tag="PC407_003110"
     CDS             4701..6146
                     /locus_tag="PC407_003110"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDF2498520.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINKRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            6457..6909
                     /locus_tag="PC407_003111"
     CDS             6457..6909
                     /locus_tag="PC407_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2498521.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
BASE COUNT         2914 a          851 c         1055 g         2324 t
ORIGIN      
        1 gcaaaattat tttagcagtt ttgtatttat ttgtatttat tttgaattta tttactaaat
       61 ttgcctataa aaagtatata taaaaatata gttcaaaatg gtataattaa gtataacaga
      121 aggaggtaat gtaagtgagc caatacataa atataaattc tcagttagac gccctaaaat
      181 tgaaggagtt cgagaaaata ttttacgaaa atatcaatga ggacgttaaa gtagtgccaa
      241 aagttactcc ttttaaagga ataaataccg atttgttgta tgttaaagat ggaagaattt
      301 tatttataaa atttatggat acaacagaag atatattttt tattttagaa gaagagttac
      361 ttgaagttat gaatgaggaa tatgaacttt tgaaactaaa aatgggacaa aagaatagaa
      421 atataagtta taactacgtt tacattatgc catacgttga agttgaggaa acatatgaat
      481 ttgaagaatt tgttaataat aatataatag ataaaaataa gcttcaagat ataatgaata
      541 agggttcgct ggaggagtat cttaatgatg agaatgatga aataaactta aatttatttt
      601 tactagacat atgttcagaa tactatataa taaatgataa acttcatcta aatgaaaaat
      661 ttaaaaaaat ttcattctac aatgatgact ataagtatac agcaactatg atggaagacg
      721 ttcaaattaa agatgttgtt tctattaaat atgggaatac acttattgaa ggtggttctg
      781 gtataggcaa aacagcaatt atgctttcta gggctataaa attagcaaaa gtatatccac
      841 atcataaatt agtcatattt acacatacaa aacaattgtg caatgagctt cgagagagga
      901 tagagttatt atacaaagat aataacaatt tagaagttca cacatttagt tcttttatat
      961 ttaaactagc taaaaaattc aatttaattg tagattataa catgttaaaa aatgattacg
     1021 aaaagacctt taataatctt gtaaaacaag cacagaacat tataaaaaat aaaaatatgt
     1081 tcaaggctat ttttattgat gaagcagaaa gctttttaga gtatgaaata gattttataa
     1141 gggagttttt atataaaaca aaattcatat ttaatgtgtg ttcttgtaac tccttgaata
     1201 tctctaatag attgaatata tttaaaaaat tatataatgg tatagaattt gatgataaga
     1261 ttatcttaag taaaaattat agacaagcta aagagattgt agattttaca aataaatttt
     1321 caaataattc taactcatat atcaacgaat taagaccaaa tacagaattt agtactttct
     1381 tttatactaa agcacttaga ggtggaaata agtctgttga tataataaaa gtaagtgacc
     1441 ttgatgacca aataagttct gtaatctggg aaattgagta tttaataagt aaaaaaggtc
     1501 ttgattattc tgaagttgct atcgtttatc cttataataa gaagaaatta aaaagtggaa
     1561 aaaccattta ctttcaatat atgttaaaaa aagcattgga agaggcaaaa attccttata
     1621 tatgtgcaga agataatttg acaaatataa gtaagaaggt aggaactaca atagcaaata
     1681 tatatgcaat aaagaattta gagtataaag cggtaatagt atgtgaatta gagatgttat
     1741 acaatcaaac tataaatgat attgaacaag attatcaagt aaatgacttt gtaggagatt
     1801 taaataaggt ttatttagct atgagtagag caacagacta cctaagtata gtaacaactt
     1861 ttaatgaaga agcaagtgat ataataagac ttattacaga atcaaaagat atttagtaaa
     1921 aaaatatttg taatttaaga aaataaaaag agactgagat ttgtaaaata aactttacca
     1981 attcagtctc ttatttttta gtccactttc ccaatattat caagaggtaa tagtctcacc
     2041 ataaccttac caagaatttc atcttcatca accataccta catcagggaa tctactatca
     2101 ttactatttt ctctattatc tcccattgca aatagtttac cttttggaac aacagtatct
     2161 atatctccag aagtatagtt attgtgtata tatggttcat ttaataattt tccatttaca
     2221 tacactttag aatttgatat ttttattctg tcaccttcag tagctataac tctttttatt
     2281 aagtcttttt tctttcctgt ttctccatca actaaattgg ttttaaaaac tacaatatcg
     2341 cctttttcag gtttaccaac cttgtatgat atcctattta aaatcagata atctttatca
     2401 tctaaagtag gatacattga ttctccactt acaacagaag gtcttataaa ttgtagaatt
     2461 acaaatgcaa gtacaatagc catagctatt gacttaatcc aatcaaatat ttctttttta
     2521 acactcataa cttttctcct tatcatttgt tattaaatta gaatacatta taatagaaag
     2581 aaataagcaa gaaccactat acccaagact attctataat aaccaaatac tttgaagtca
     2641 tgttttttga tatagtccat aaaaaattta ataactacaa ttgaaacaac aaatgctact
     2701 acagaaccaa cacctaaaat aagccattca aatccagtaa atccaaatcc agcttttaga
     2761 agttttaatg cactagctcc aagcattgta ggaatggcta agaagaatga aaactctgct
     2821 gctacatatc tagaagtacc aagtaatact gcacctatta tagtagaacc agacctagat
     2881 gtacctggta tgagagctag acactggaat aatcctatac aaagagctaa tttatatgta
     2941 acttgagaaa aatctttata cttaggtctt ttatttctct tttcaagccc tatcattatt
     3001 ataccataga caataagagc aatagcaact actgttgaat taaagaatac tctgtctatg
     3061 tcatcttcaa acagtattcc aataactcca gatggtataa ctgcaattat aaccttaagc
     3121 cataaacgaa ctgtttcttt tttctgttta atattctttg cactatcaaa aggatttaac
     3181 tttctaaaaa atataacaag tacagctagt attgaaccaa attgaattac aactaaaaat
     3241 gtatttataa atgtatttga aaaattcaat tttataaact cattaattaa tatcatatgc
     3301 cctgtactac ttactggtag ccattctgtt attccctgaa ctatgcccaa aatgactgcc
     3361 ttaaaaattt caagcaaact aaatgtttcc aaatcaaaat acctccaata caaatttata
     3421 gtagcatatt ttattcatag attaataaaa aaatgccacc ttttactaac ttataatacc
     3481 atagtactgt gtagtgcaca atggaagatt gatattttca taaaaccttc ataatataac
     3541 aaaataaact taaaagttat ctattgaata aggggtatat ttgaggggga aatatatggt
     3601 atagtaaaag tatagctatt ttattttatg aaaaggaaag gagttattat gtatagtaca
     3661 aaaatactag atgattatga gtatgaaact tttaatgttt tttctacaaa attaaatcca
     3721 aacacgaaac acgattattt atcaaaggtt atactgttta aagaattttt aaaagggaaa
     3781 gaattgattt atgctacaaa agaagattgt aaaaattttg tagactatat acagactaaa
     3841 tatgcgaaat caacttgtga aaagatatat agttatttac atagttttta taacttctta
     3901 aaaaaagaag gatacataga tataaatcca tttagatatg tagaaaaacc cacagtaaca
     3961 agaataaaaa caaaagatga tgttctaagt atacaagaaa taaataaact tataggtatc
     4021 ctaccaaaat taaatataag agatagagta ataatagtct gtctagttac tactgggtgt
     4081 ttattaaatg aattagttag cttaaagtgg aaagacctta tggtagatga aaacgataac
     4141 tcatatgtaa ggttaggtaa gggaagaaaa gaaagagtag ttaaattaca tccatacttt
     4201 tttaaacttt tagaagatta caggaattat tctgggcttc cagaggtaat aatacctagt
     4261 gatgatttta tttttacaac tcaaaagagt aattctataa cagatagaaa tgtcagactt
     4321 atagtcaaaa aagcattaga tttagctgga ctttctcaat attcagcaag agattttaga
     4381 cattcatttg cagcaataag tttaaggctt ggggcagatg aaagtgatgt aaaaaatcag
     4441 cttggatgga gtgataaata ctatgcaatt agatataagt atgtattaaa ttttgttgat
     4501 agtgaaatag tggattactt aattgaaaaa gataatttgg atataaataa aaaatagtaa
     4561 taaataaaaa atactatata tagatgtgaa caaaaaataa aaaagtcttg ttatagttcg
     4621 taaaattata tataattatt tatgtactaa tgtgtacaca gtaatcacac gacaaaagat
     4681 ttaaggaagg tgctaatata atgatagata ataaatatac tacttactca aacccactta
     4741 cagacagata ttgtagcaag gacatgagtt atatattttc tccacaattt aaattctcta
     4801 catggagaag gttatgggtt gcacttgcag aaggtgaaca tgagttagga atcaatataa
     4861 ctgaagaaca attagatgag ctaagagcta atatagataa tataaatttt gatgaagcta
     4921 gaaaaataga aaaagaagta agacatgatg taatgtctca cgtaaaagct tatggtttac
     4981 aatgtgaaaa agccaaagga ataatacatt taggagcaac atctgcatat gttggagata
     5041 atacagatgt tatacaaatg aatgaagctt taaaacttct aagagtaaag cttgtaaatt
     5101 taataaacaa tttaaaagaa tttgcactta agtacaaaga tatgccagct cttggattta
     5161 cacacttcca agctgctcaa cttacaacag taggaaaaag agcttgttta tgggctcaag
     5221 atttaattct tgatttggaa gatttaaact atagaataga taacatgaag ttaagaggag
     5281 ttaaaggtac tactggtact caagcaagtt ttttaagctt atttgaagga gaccacgaaa
     5341 aagtaaaaga gttagataag aaaatttgta aaaagatggg ataccattct tcctatgcag
     5401 taagtggaca gacatatact agaaagttag attatcaagt aataagcata ctatcaggga
     5461 tagctcaaag tatgcataag atgactaacg atataagact tttacaaagt ttaaaagaat
     5521 tagaagagcc atttgaaaaa aatcaaatag gttcatcagc aatggcttat aagagaaacc
     5581 caatgagaag tgagagaata gcatctttat ctaagtatat aatctctgaa tctataagcc
     5641 ctgcaatggt acaagccact caatggttag agagaacatt agatgactca gccaataaga
     5701 gattggcaat acctcaagca tttatggcag cagatgctat acttgaaata gggataaatg
     5761 taactgatgg tttagtagta tatgaaaata tgataaataa acgtgtaaat gaagaacttc
     5821 cttttatggc tacagaaaca atacttatgg aagctgtaaa aagaggtgga gatagacagg
     5881 aattacatga aataataaga gaatattcta tgaaagctgc ctatagagta aagcacgaag
     5941 gtaaggataa taacttaatt gaacttataa tgaatgatga ttcatttaaa atgagcaagg
     6001 aagaaatact atctataatg gacccaaaaa actttatagg tagagctcca gaacaagttg
     6061 tagaatttgt aaatgaagtt gtagaacctg ctataaaaga ttataaggaa gacttaggaa
     6121 aagtagatgt ggacctaaga gtatagaatt gtacagataa aatataatta aaaacggcta
     6181 tcttatttat aaataagata gccattttaa tgggctagcg tcttctcttc agcgctgtga
     6241 agagttcttc tttacttact ataccatgaa tttaataaaa tagctatata aataatattt
     6301 aatagaaata atataaataa aaataaaaaa atgataagat tactaattaa atttaaacat
     6361 tcagcaaaaa ctattgtact tgcaatattt ttaatgtaaa ctgaaataaa gttatttaga
     6421 taatttaata aattttaaaa caaccgaggt gattatatga actatatagt aaaagatacc
     6481 ataagttttt tagtaaaaac atttcctaag atttattcta gtctgtactt agaagaccta
     6541 aaaaaatttg caccagatta taatgtaaat aaaacacaat taagagcatt agttttcata
     6601 aaaaattatg gagtaataag tatgactgat ttatgctcta agttaaatat agaaaaaggc
     6661 agtttaacta gtatggttga tgatttaact gataagaagt atgtaacaag gaaaagagat
     6721 ttagttgata gaagaaaata tctaattgac attactgaag agggagataa gatagcaact
     6781 gattttatgg ataaattaag tgatggttta ggagaaaaac tttctaaatt aactgaagaa
     6841 gacagaaaaa aatatttaga agcaataaat actcttcaat atatattaaa caaagaagaa
     6901 tttagataga aaaaatataa aaaattttta tatttaatag tactaagatt aattctttgt
     6961 tattcattta actatataat tataagtcta aatacaatct aaaaagtaca tcttaaattt
     7021 tcttgatgta cttttttata atttcttata cattttctta tttgttagta cctactcata
     7081 cctctttgtg taatttatat ttttccattt tatattcaat aattttattt tctaatctct
     7141 tttt
//
LOCUS       DAMJVX010000174         6990 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMJVX010000174 DAMJVX010000000
VERSION     DAMJVX010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.180"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     repeat_region   474..1160
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=540..568
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            complement(1552..3285)
                     /locus_tag="PC407_003112"
     CDS             complement(1552..3285)
                     /locus_tag="PC407_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2498522.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDVFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     gene            complement(3525..4370)
                     /locus_tag="PC407_003113"
     CDS             complement(3525..4370)
                     /locus_tag="PC407_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2498523.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            complement(4401..6635)
                     /locus_tag="PC407_003114"
     CDS             complement(4401..6635)
                     /locus_tag="PC407_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2498524.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQYIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESSRELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLILFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
BASE COUNT         2400 a          919 c          867 g         2804 t
ORIGIN      
        1 ttttttagat agtaaacttt aaaatataga tattaattat atgaatataa taaaaaaagt
       61 actaatgagt tacactagta ctttataact acttttacat gttttaactg tataaaacag
      121 tgggtatagt tcagcaggag tgactttagt tttgaactaa aaatcaagtt caaaaagaat
      181 aaacattagt atttgaactt cactctacgt ctaaatagat tgtagttctt cttgttttta
      241 ttataccaca aattggtaca gatattcaaa aataatatat ttatgatata ataaaaatgt
      301 aaatagtttt gcagtgagcg atatttgtta caaagtaggg cttaatgctt gaaatataag
      361 gtgttgaggg tatatgataa actttatcat ttgcactact catggttcac tgcaaattta
      421 agagagttgc acatgtgtaa gtattgaaaa tgcccagttt attttggggt aaagttttat
      481 attaactaag tggtatggaa agaggttttt cagaagtata tgtacgtcta tattgtaaag
      541 ttttatatta actaagtggt atgtaaataa ggaaggtgga agtcaatatg catgggtcaa
      601 atgggtttta tattaactaa gtggtatgta aatacgaaat tagagtttga aatatatgac
      661 tacataatag ttttatatta actaagtggt atgtaaattt gtgtcacatc attgtcactt
      721 ctttagaata tcaaaagttt tatattaact aagtggtatg taaattttta agcaggtgat
      781 taaatgatta aaaaagtaga aaagttttat attaactaag tggtatgtaa ataataactt
      841 gtcaatgtag ttatatgcaa caacttctat gttttatatt aactaagtgg tatgtaaatg
      901 aattatatat tatgaaaaaa tatggagtca cagagggttt tatattaact aagtggtatg
      961 taaatttgca ggttttcctt atagtttgcc atatgttcct ggttttatat taactaagtg
     1021 gtatgtaaat tactttagca ctagtaaagg tagaattatt aaagtgtttt atattaacta
     1081 agtggtatgt aaattcgaat gtgaatggca cttctatttc tccaactttt ggttttatat
     1141 taactaagtg gtatgtaaat agtataatgt tgaaaagtta gagagtacaa tcaagaaatt
     1201 ttacattaac taaaatgtat agaataaaac atgtatttat acttaaattc tgtacctata
     1261 taaaaaagtg aactctgtca acaaagcact tttttatata gataaattat cattttattt
     1321 taagatagaa gatactaatg ctaaatgttt atcattagta tctgtatgta cataaaagtt
     1381 taatttttta tataaatttg atctttagaa aaatgagcag tatcaataaa tatattgtct
     1441 aaattttttc taggaactag ttgactagct ataagattag cttcaactct ttgattgtta
     1501 gactatgaaa ttaaatctaa aggttcattc ttggtcgtat aaatagcttt atcattcgta
     1561 tgtactataa caatttttgc catctgcttt tgatagataa agagctttat cagctttaga
     1621 aaataaatct ttatataatt tagttgaatc atcagtgaag gcaataccaa tacttaatgt
     1681 tattttatga ttgtccttta cttttatttt acttgcatca tttaaaatat cattagtaat
     1741 ttttattatt aaatcctttg ttaatgattt tataaatatt gtaaattcat ctccaccaat
     1801 tcgagcaact atgtctttat catgtgtgtt atgtaatagt atttttgata cagcaactaa
     1861 aacttcattt ccctctaaat gcccaaaagt atcatttaca gttttaaaat catcaatatc
     1921 aataactaat agagcatcat tatttttgga atcagaacta catagatact cttcaataag
     1981 attttgtgat actctttgat tatataattg tgttagtggg tcacgttgag cctgattaat
     2041 taagctttct aatttagatt tatcttcagt tatgtctttt aaaacgccta ttgttttgat
     2101 agggttatgg tcactatcat atataactga acattgaaca cgaatccatc tccattcatt
     2161 tttagttaat gaatttactt gtcttatctc aattgtttca cgaagatttc catgaagtac
     2221 ttctagtagt tttccaatat catcaaggtg aataatttta ccagattcta atagtttgga
     2281 gtaatttttt gtttcaaaat tttctaattc aatttttttg tcaatttcta cacgttgata
     2341 ttttataagg gtatcgtttt caatatcata ctcaaacatg atatctgtaa tattttgcaa
     2401 agcaatacga tatcgctctc tttctaattt tagttcagat tctatgtttt ttagttgggt
     2461 tatgtcagta ataatactat taatttttac tatgccatca ctatttaacg atttcattcc
     2521 agaatctaat acccacatta atgttccatc tttttttcgg attctatatt cagatgaata
     2581 tgtaggtcct tttgcaaaac atcgttcaca atcttctagt gctttaggta agtctggtgg
     2641 atatacagca cccactgctg aaccaccact catttccatg aactcatcat atgtgtatcc
     2701 taatattttg ggcaatccct cattgacata gaaaaatgag tatgtactat catcattgct
     2761 accttttaag ccaccattga tattatttgt aatcatttca ataacttctg ttttttcctt
     2821 tagtaatctt tgcaaataat cataactttg agttccaatt ttagtgggaa agtattcatc
     2881 aagttgcatt tcaccataag ggtttgatat gtgcatgtgc tttactaata attttcctct
     2941 atcctcaatt atctcaaatg tacaacgttg atttacctct aaaatcattt gagcattttc
     3001 tttagttcga acagtatatc ttcctaatac catacatcta ttctcatctt cacttacagt
     3061 ttcaaataca tcattattta tatcacaaga aggaatttca tcttgacctg cataaaaata
     3121 attttttatt tcattaaaag aagtgagaaa ttcctgttct cctggtccaa tccatgtaac
     3181 attttcacaa aggtatttta atacttcatt gatatctcga tttatataaa aacttgttat
     3241 aatatgtttt gctaatttgg gtgctttttt taggagtatg ctcatgatat tcctcctttc
     3301 tttttgctat tatattttaa ttatattata catcaatata tttaaattaa aagctttgtt
     3361 aaactatgat atctttgtca aataagttaa ttttaacaaa aaagaactga aacaatatat
     3421 gcttcagtac ttaatgtcaa tatcttgtat agatacatat ttataaacta atgtacaagt
     3481 ttaaaaaaag aaagataatt ttaatttatt ttaaaataac aatgttaata atcattttta
     3541 tcaaattttt tcttgttttt ctccttgttt ttatacattt ttttatccaa ttcaactatt
     3601 gtcaatttta aatttctttt tactgtatca acaccataac taaatgaaag atacttatat
     3661 ttagagaata cttttaaaaa attttctctt aaggcttcta gttgtgaata aacataattt
     3721 ttgtcaacat cactagagaa tataacaata aattcatctc ctcctaatct tataatagaa
     3781 gtagaattta ttaattcttt atttaaaagt gaaacaaaat cacataaaat cttgtctcct
     3841 acattatgac cttcatagtc atttatcatt ttgaaattat ctaagtcaat tagtacaata
     3901 taatctccta taaatatgtc aaatctatca tttaaatatt ttctattata tgctccagtt
     3961 aatgggtcag taattgagta gtgaagattt ctcttatttt cttcatagag ttttgttata
     4021 tcattggcca tagtaattat gtaggttttt tcatcaacat ctactttgta ttctaaaaca
     4081 ttccaatagc ttgtagtatc ttttttaaac tctaaaatcc aatttgaaat tttatcataa
     4141 ggataatttt tttcccaatc taattgaata ttgtgagaat ccgtaaaaaa tttagcaata
     4201 ttatcaggga gtttcttagc tatttctata ttagtcattc caagtggatt atcttgcata
     4261 taacttttaa ttaaattttt taaattatca tttataaata taattctgta gttagaatct
     4321 cttataatta gaaatcctgg aaaagttctt aaaaaaattt ctttaaacat attaatttct
     4381 ccaaaataaa atactttgta ctagattttt gttaaatttt ttattttatt tacatctaca
     4441 tacatacctt gggcaatcga ccttccatca attgcagaaa ggttacattg tgtatacata
     4501 actgtgacaa tttgaccatc tcgttttatg aatctatata aaaatggttc aattttattt
     4561 gtgtcaatca tatccaaact ttttttataa acaaagtcta aatcatctgg atgaacaatt
     4621 gctcttaaat ttccgcccat ctcaccaaaa aactgttctt ttgtataacc tataatgtca
     4681 aacatttctt cattcatata agtaataatc atatcaccaa ataaatcgta gcgaattata
     4741 cctacaggca acattgaata aaattgtgat aaaatattac tttcatcaag atgtgtattt
     4801 gtcattttag gtattgaata tgattcaaat tcatttatat agccagcagt tttaattaaa
     4861 tccaaatgta attttgctaa gagtaattta ttttctgaaa ttatacaatt tgcactaaag
     4921 tagaaattgc tatatttttg atgttcagaa gcttcatctg ataaaacggc ttctccacta
     4981 attaataatg aatctgattc agtattatgt ggggttatac ttttgtaaag aatagaaaat
     5041 tttttacctt taatatgttt ttcaaaatgt tctattactt taggaaaaga tgtaatttca
     5101 gttttgttgt aaaaatcaaa ccattgaatg tcttcagtga cattagctct tagttttttc
     5161 atatcttgca taataaaaaa tccatcaaat atcttctcta ttaaagattt tcctatatca
     5221 gaatatttta taaacatttt ctctgaatta ttctctttag atagtaattt ttcaaattct
     5281 ttgcactcca tgggttttgc ataataataa ccttgggcga tatcacaatt tatactttta
     5341 ataaattcta catgttgttc tgtttcaaca ccttcacata ctgtaagcat cccaagagct
     5401 cttgcaatgt ttattataga gtacaaaatg gttgtatttt taaaatcatt taaattatga
     5461 gataaaaata tcctatcaat tttgattata tcaaaaggta gattttgcaa cataccaaaa
     5521 gatgaatatc cagaaccaaa atcgtccatt gatacagaaa aaccataaga acgaagttca
     5581 gctagttgtt gaataattgt atttgtattt tctattaata tattttctgt tatttcaatg
     5641 ctaaaatttg atgtagagac gctgtacttt tcagtaatac cttttagcat acttggaaaa
     5701 ttagggttaa aaaaatgaag acgagaaaag ttacaacaaa ttggaacaaa aacatcattg
     5761 ttatcaatcc atcttcttat atttttacag ttctcttcaa ataaatgcaa atctaattcc
     5821 gttatcagac caaactgttc aaataaagga ataaatttat ctggagtaag aagtccttta
     5881 atagggtgtc tccatcttgc aagtgcctca catccaacta tagtattatt ttttatatta
     5941 taaagtggtt ggtaaaatac ttcaatttgt ttagtttgta gaacatgtga tatttctgat
     6001 aagaactcct tatcattttc taatttattg taaaaactat tatctttaaa atcaaataga
     6061 atgtatctat ttttgccttc acttttagcc ttttttaaag cactatatac ttggttgaaa
     6121 agtatcaaag tagtcatgtt tggttcacca ttccagatag tgctgccgat actaaatgta
     6181 acattggagt atatttcttt ttcattagga agtcttattt ttaattctcg ggagctctct
     6241 ataatagttg cacccatatt atttaactct ttatttgata catttggaag tacaattaaa
     6301 aaaccttctc cttttaaata agcgactatt ccctttgatt ctggaataaa gtgcaatagg
     6361 ttttgagtta gagtaattaa taaattttct ccgactagat atccatatat atcattaaat
     6421 aatttaaagt tatcgatatt taataacatg attcctatat gtatattttt cgaaatacag
     6481 tcatcaatat attggtacat aaattctctg tttggtattt tagaaaaaaa atctatttca
     6541 tttaaggttt ttaatttaaa atttctacta tctaattcct tagataatac ttctaccttt
     6601 ttatttaaca tttttatata attatgattt tgcatggtat tcctccataa gatactttaa
     6661 attttgatga caactgtatt tatgctagaa gtatttaaat gttaatttgt aaataattta
     6721 aatgctaaat tttaaataag tatttaaaat gaatatacat taagttattt gctccaatgc
     6781 tacaaataca aatatttttt agtttagtaa taatattaga taaaattagt taatgcttaa
     6841 attatagtat attgaaagat ttcctttttg aatgcaatta tatcatattt tttcataaat
     6901 agatagattg tgagctgtat agttagtaag gtagagtttt tttataatta attttaagtt
     6961 taatgatttt tcaaaggcat aattatttta
//
LOCUS       DAMJVX010000175         6714 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMJVX010000175 DAMJVX010000000
VERSION     DAMJVX010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6714
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.181"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(<1..1679)
                     /locus_tag="PC407_003115"
     CDS             complement(<1..1679)
                     /locus_tag="PC407_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498525.1"
                     /translation="MKYLIEIAKKERLLVFIYLALGIGIELLNSFSANYYQSLIDRFN
                     NSTINFHIIFIYGTVLVTLCLLRYIDEYPGRRLEHSFFLDLKLKALEKMSKIDYLEYQ
                     MLGTGKLIQKIENGANAGRNIFFNFLLAVVRKIIPSILFSMVFIYRIDKKVMFTILIG
                     YFIVFIITNLLIKALYQIKEHILINEEKMNHFLVRGFVEMVVFRVNKRFEYEICQSKF
                     AKKEIIDSRVKMTLIHEAFFTIFSLIVTFIKMLIIVYSWKTKTISIGSIIALITLLDN
                     AYTPIAIFNVLYIQFKLDKAAYKRYTDILELNNDGQLLAGKEVNLMYGNIEFNEISFL
                     YNNKREIFNKLSLSIKSGEKVAIVGESGSGKSTLIKLLIGLLKPSEGTIFIDDYNLLE
                     MNLNSYYNYVTYVSQDSPIFDGTLRENIVFGKTIKSNDIIEVLEKVGLKNLYLKLEAG
                     LDTKLGEKGTTLSGGEKQRLALARLWFSDAKIIILDEATSALDNITEEKVMLNVMEFL
                     KDKTTIMIAHRFNSIKNVENIIVLKEGSIIGSGSFKKLLDGNLYFRELYYT"
     gene            complement(2602..3183)
                     /locus_tag="PC407_003116"
     CDS             complement(2602..3183)
                     /locus_tag="PC407_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2498526.1"
                     /translation="MNNKKHWYDYLWIFSSIYLVLGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(3176..3406)
                     /locus_tag="PC407_003117"
     CDS             complement(3176..3406)
                     /locus_tag="PC407_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2498527.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            complement(4731..5006)
                     /locus_tag="PC407_003118"
     CDS             complement(4731..5006)
                     /locus_tag="PC407_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2498528.1"
                     /translation="MLELTKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            complement(5082..6383)
                     /locus_tag="PC407_003119"
     CDS             complement(5082..6383)
                     /locus_tag="PC407_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2498529.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
BASE COUNT         2302 a         1231 c          548 g         2633 t
ORIGIN      
        1 attgtatagt ataattctct aaaatataaa ttaccatcta atagtttttt aaagctacca
       61 ctacctatta tactcccctc tttaagaaca attatatttt caacattctt aattgagtta
      121 aatctgtgag caatcataat agttgtttta tctttcaaaa attccataac atttagcata
      181 actttttcct ctgtaatatt gtctaaagct gatgtagctt catctaatat aattatctta
      241 gcatctgaaa accaaagcct tgctaatgct agcctctgct tttctccacc tgataatgta
      301 gttccttttt cacctaattt agtatctaaa ccagcttcta attttaagta taaatttttt
      361 aaacctactt tttctaagac ttcaataata tcattacttt ttatagtttt tccaaataca
      421 atattctcac gtaaagttcc atcaaatata ggtgaatcct gtgacacata tgtaacataa
      481 ttatagtaac tatttagatt catctcaagt agattataat catctataaa tatagttcct
      541 tcgctgggct ttaataatcc aattaatagt ttaattaaag ttgatttacc agaaccactc
      601 tctccaacaa tagctacttt ttctccactt ttaatactta aactaagttt attaaatatc
      661 tctcgcttat tattatacaa gaagctaatc tcattaaatt caatatttcc atacattaaa
      721 ttaacttctt tacctgctaa cagttgacca tcattattaa gttccaaaat atctgtatag
      781 cgtttatatg ctgctttatc tagtttaaat tgaatataca atacattaaa aatagctata
      841 ggagtatatg cattatctag taaagttatc aatgcaatta tagaacctat acttattgtt
      901 ttagttttcc aactataaac tattattaac atttttatga acgtcactat taatgaaaaa
      961 atcgtgaaga aagcttcatg aatcagtgtc attttaaccc tagaatcaat aatttctttt
     1021 ttcgcaaatt ttgactgaca aatttcatac tcaaatcttt tgtttacccg aaatacaacc
     1081 atttctacaa atccacgaac taaaaaatga ttcatcttct cttcattaat taatatatgc
     1141 tctttgattt gatatagagc ttttattagt aaattagtaa taataaaaac tataaaatat
     1201 ccaattaata tggtaaacat aactttttta tcaattctat aaataaaaac catactaaat
     1261 aaaattgatg gaattatttt tctaacaacg gctaataaaa aattaaaaaa tatatttctg
     1321 ccagcattag ctccattttc aattttttga attaattttc cagtgcctaa catctgatat
     1381 tctaagtaat ctattttact cattttttct aaagccttta atttcaaatc taaaaaaaaa
     1441 ctatgttcta gtcttctacc tggatattca tcaatatatc ttaaaagaca taaagtaact
     1501 aatacagttc catagataaa aattatgtga aaatttatag tactgttatt aaatctatct
     1561 attaggctct gataataatt agcactaaaa ctatttaaaa gttctattcc tatacctagt
     1621 gctaaatata taaaaactaa taacctctct ttttttgcta tttctattaa atatttcata
     1681 actgtacacc tatccttatt tattttactt tattaactta aaataatttt atagataaaa
     1741 tgtttcttta tttgtttaaa tttagtgtga ttattaatca tttcaagtta acaaaaccta
     1801 aagtttttag cagtacagtt ctatttgtta gcaaattatt attttactca gtacattatg
     1861 ctaacatata atctaactgt actgagtaaa tatctcgttt catcatataa aatctccttt
     1921 ttatagttta atatatatag ttaaatttac tatataatct gaatatttat ttttctatca
     1981 cctttaccag aaataaaatc aattatttat tgtataagca aaacttcata atattttcaa
     2041 catataatta ttatacagaa ctcctaagtt aatttaaagt tagaatttat ttttataaga
     2101 aatgtcattg acctaccttt aataaaacag ccaccatttt tttatgtatt aacatataat
     2161 atatagtaat gaaaacatat taattaaaaa atattgtata aattagttta tctgtaacaa
     2221 atccagttta tgtttcaaca agcacgtaag acttcttgaa atttaatctg cttcctttaa
     2281 atatctatta cttgttttaa gcataagttt acaatcttct ataacattta tcatttcttc
     2341 agatttctct gacgtactta aaatacaagt tgttacattg tttttctatt taattagtaa
     2401 tgtatcatgt aaaaaagaag cataaatgag tttactttat acaaacttca tttatgctcc
     2461 actcatatac ctatcatagc taatagaaac tattactcat cttgaatcta tttcatattt
     2521 agatatttat atctttctta ttttcttaaa tcaactctag ctttaaacat taaacccctt
     2581 aaatcttcac aaaatctctt cctatacaga cttactcttt gccttacata tcatctgagt
     2641 catagtaccc atagggcaat aaacacacca tgaacgaggt ttaaacaata tcatagtaat
     2701 caaaccaaga acagttgatg taagcataac actataaaaa ccaaatgcaa actgtaccac
     2761 ccaaggtgaa actaaagttc catgatacgc ccattgccaa ggcattttta tagtccacaa
     2821 caaagtcact acttctttaa aatttctact tccttcaaac actaaataag ttgaaaaaag
     2881 catattggca aacattacca taaaaaatgt caaaaaccca tatctaaacc acttacttct
     2941 aataaattta ggaatatctc tatttcttga cagcttcaat ttatttccaa gcaaattaaa
     3001 cagttgccct cttccacaat acttattaca gtaagcttta tttcccttaa caatagaaat
     3061 agctagtgga acaaagaaac aaattagccc aatccaagcg aaaataatat ttacaaatcc
     3121 caataccaaa tatattgaag aaaaaatcca taaatagtca taccaatgtt ttttattatt
     3181 catgacactt accttcttcc tcttttaata ttgttataat ctcaattaca gatgctggac
     3241 atatcttttc acaaatccca catccaacac ataaatctct attaattttt gcactaatgc
     3301 cacatggaac tgagatagca cttcttggac atgcttttat acagctacca caagcaacgc
     3361 actcatttgc taaaatattt gcatacttct tttttttatt agacataata acctccctat
     3421 attattaatc tataagaatt atatcaagat acttactttt tatctgtgac ttaatcacat
     3481 aaaacctcaa tattaaaaat cacactctaa atacaaagga gtttcaaata aaaaacgata
     3541 tgaagtcatt aagtttagct tcatatcgcc ttttcttttg tgtatctata taaatattta
     3601 ttatctaata tttaatatat ctataatatt ttcttataca gctctataat tttacaccta
     3661 atcatattct attagtaaca ggttaatacc catctaaaac tataacttta ttcacaatct
     3721 tattctatta gccacaagtt aaactcatat tgaagtttat ctttagttac actcttactc
     3781 tattaatcac aagttaagcc catattgaag tttatctcta tttacactct tactctatta
     3841 atcacaagtt aagctcatat tgaagtttat ctctatttac actcttactc tattaaccac
     3901 aagttaaact catattgaag tttatctcta tttacactct tactctatta atcacaagtt
     3961 aagctcatat tgaagtttat ctctatttac attcttactc tattaaccac aagttaaacc
     4021 catattgaag tttatctcta tttacactct tactctatta accacaagtt aagcccatat
     4081 tgaagtttat ctctatttac attcttactc tatcaatcat aggttaagct catattgaag
     4141 tttatcttta gttgcacctt actctattaa tcacaagtta aacccatatt gaagtttatc
     4201 tttagttaca ccttactcta ttaaccacaa gttaaaccca cattgaagtt tatctctatt
     4261 tacactctta ctctattaat cacaggttaa acctattata atcttaactt tattcacaat
     4321 tttattctat tagccacagg ttaaatctat tataatctta actttattca catattacat
     4381 cattaatcgc atatcaagat tttataataa gctttaacta aacacacgat tttaccttat
     4441 tcgtatagtt taatctatta aattattata taatcttaag caaacataca actctacact
     4501 attaattata gcccctcctt ttaaatactc ataatcttat acacaacctt accttattaa
     4561 tcacacattt aactcatata tatcatcaat tttattcaca aaatcatgct gttaatcaca
     4621 cttatactcc tctataatct taaacttata cacaaattag ccacaatttt atttcaacta
     4681 taataacttc tatttcagaa gtatatcaca cttgtattct ccaaaaatat ctattttttt
     4741 ctaacaatat gcctgacatt aataacactc atttcaaaca tcctcgctaa ttccatataa
     4801 tttttcccat taaacaattt aatcataagc tcatttctag cctcttttaa tgccccttcc
     4861 agtgttttaa tataaaaata tgttccccca ctttgttgac acaattcaag gtattccaat
     4921 aaactatact tatgtaactc ctttggaata cttacttcta tcttgtgttc ttcaagtaat
     4981 tctataattt tcgttaattc caacatattt tcacctcata ttttataatc tatttgctat
     5041 ttcctgactt ttaataagct tgttattact actttattga cctaataatt tagttcttga
     5101 aaatgtaact tatccccaaa gtaacaaaaa tgcttatgcc tagtctgtcc atctctacac
     5161 ttcgcaacta ttaaatcaac catcttaatt cccctgtatt ctgatgctct aactgcctcc
     5221 tcactttctc caatatctct cactgcttct tcataatcac tcccctttaa ttgatgtata
     5281 taaaccacat tgttgctatc gtggtagatg gctttgctat ccctcatagg tctatctcca
     5341 taaggtcttg aatctttcat ttcatcattt aactgactta attggattac aggtatatta
     5401 aaatctaaag tcatattttt aagttctctc gaaaaagtag ctacctccct ttctctctta
     5461 tccatgcttt tctgagatgt aaggagttgc acataatcca ctattaataa atctggcttt
     5521 acctgcctta tcctctttct aattgcacta attgtatcta tcttatcatt aatataaatt
     5581 aagttatttt cgcttaaatc acctatagca tttacaataa gtttccaatc attttcatct
     5641 atttctttag atttcatatt ccttgtactt acaccagtta ctctgcatat atttctcata
     5701 aatacttgct cactggtcat ttctctactt ataaataaag tttttttacc ttgcttaaat
     5761 gaatttaaca ttatttgaag tgccaaagca gttttcccaa cccccgattt agctgctatt
     5821 gtggttagct ctcctttaaa aagacctcct atagtagtat caagtaattt tataccaaat
     5881 ttaaatccaa tatctttttt gttttctaaa aaatcaagca ccttccctgc tatactattt
     5941 acatcatcat tttcataagt atccttgtcc aaaatctcct ttatattact ttcaaatttg
     6001 taaatacaag tatcgatatt ttctccaagg tttaggctgt gtaaaaggtt ttggcagttt
     6061 tgatttatgc ttcttctagc taaattttct tttaaaattt taatatgacc atcaagacca
     6121 aaattctgac cccaagtaat tagattagtc acatcactcg tttttattgc tagagatttt
     6181 ctatctatct ttgatttcac agtggccaca tcaatcacta tgttttcttt tttaagttct
     6241 ttcataattt taaatatctc aagacatata tcattcataa acatgttttc atttaattca
     6301 tctagcttat agcttaattt attgtctaaa agaattgtgc ctaaaataga cttctctatt
     6361 tcaacatctt ttgatttacc catataaatt aatctctctt tccttataag tttttatatt
     6421 ttgattttta aatacattat caattataaa tacattattt gttatgactc actatttgtt
     6481 atgactatat tattcgtcat gatatactac aaattattaa tatactattt attctgatac
     6541 atataattta ctacataata gcaatatctt atttgatata acttactacc aattattaat
     6601 acattgttca atataattca ttactcgata gtaatatctt atttgatata acttactgcc
     6661 aattattgat atatcgttca atataattca ctacctgata gtaatatctt actt
//
LOCUS       DAMJVX010000176         6700 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMJVX010000176 DAMJVX010000000
VERSION     DAMJVX010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.182"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            207..1040
                     /locus_tag="PC407_003120"
     CDS             207..1040
                     /locus_tag="PC407_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2498530.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1133..2065
                     /locus_tag="PC407_003121"
     CDS             1133..2065
                     /locus_tag="PC407_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2498531.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKEYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2117..2599
                     /locus_tag="PC407_003122"
     CDS             2117..2599
                     /locus_tag="PC407_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2498532.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2611..3480
                     /locus_tag="PC407_003123"
     CDS             2611..3480
                     /locus_tag="PC407_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2498533.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGVIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKAHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3523..4278
                     /locus_tag="PC407_003124"
     CDS             3523..4278
                     /locus_tag="PC407_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2498534.1"
                     /translation="MTTYKKLKKSIFKISIFSICLLVSLVDINPIFAGSSSNETSNSD
                     TIKVLNAKSLNFKQGNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEDSSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDFPVKDFESINKYQDFYFNKN
                     GNLVISFEEYEVVPGYMGAVEFVIPNKVIKRLR"
     gene            4320..5141
                     /locus_tag="PC407_003125"
     CDS             4320..5141
                     /locus_tag="PC407_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HDF2498535.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANYER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKRETGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5146..5907
                     /locus_tag="PC407_003126"
     CDS             5146..5907
                     /locus_tag="PC407_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDF2498536.1"
                     /translation="MNTGSKDIDTLNVNFRGIEIKAVLVYKNRKNISIKIDPFGKIIV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKYKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLKDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERTVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6068..6325
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6438..6677
                     /locus_tag="PC407_003127"
     CDS             6438..6677
                     /locus_tag="PC407_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2498537.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
BASE COUNT         2956 a          629 c         1135 g         1980 t
ORIGIN      
        1 aaaacactat ataatactca attttcaaca ttctatacaa ttatacagaa attatacagc
       61 ataataatca ccgaaataac aaattttaaa aattaagcaa aactttttgt cgaagttcaa
      121 cgtctatata tatgtagtgt aaatttttgg gataaaatac tacaaaaata taaatgtaaa
      181 aatatagtaa tattggaggt cgaacgatga gaaataaaaa gaaaaaaata aatagaaaga
      241 agttgtattt gcttttaagt gctattgtag tgtgtctggc ttttatagta gttggtgttc
      301 gcgtcagctc tttaaatatg aaggaaaatg aaattagtag aaatgcaaaa ctgtcaagtt
      361 ctactattac agatatagta tctaatacat caatagaagt tagtaaaggg ccaagtaaaa
      421 gttctggaaa aatagcatac ataactatag atgatggccc atctaaattt actgacgaga
      481 tgataaaaac tttaaataaa tacaatgtta aagcaacttt ttttatgata gatgggaata
      541 tgaaggaata tccacagcaa gttaaaaata taattaaaaa tgggaacaca gcaggttttc
      601 atagtgtatc acatgatatt cacaagcttt atgttactag tacatcagca aaagaggaat
      661 ttgatacaaa tgatcaaact ttttataaga taactggtaa acattcaaaa gtaataagaa
      721 taccttatgg aagcaaacca tatacaccac aagcctctta tcaggcttta gttgatgctg
      781 gatataaaat ttgggactgg gacttagata cagaagattg gagatctaat tcttctcaaa
      841 ttgtacaaaa tgtaaaaaat catattaaga atagaaaagg tgaagataaa gaccaacttg
      901 tagttttaat gcatgagaaa aaacagagtg cagaagcttt agattcagtt ttaaaattct
      961 tatcagatga aggttatgaa tttgcgccag tagatcaaaa ccaaatacca aaaaattatt
     1021 ggttacgtaa tttagagtag ttaaaatata agaatattga ttgatatttt tagaaattga
     1081 gaataaaaat gagtgaggtg taggtatatt gaataaaaaa ctttatatag gaatggtcgg
     1141 aatattgtct ttgatgatgg ttggttgtaa taaaagttta gcaaaagtaa atgatgtgga
     1201 aatcacaaaa gaacagtaca aaaaaacaaa agcagtttta tctgctacta ataattatat
     1261 aaatgggcag tctttagatg aattagaaaa aactttggat aaaaaaggta gaaataaatt
     1321 agaaaatgtt ataatatcgt ttatggtgga taatgaactt ttatatcaag aggctaagga
     1381 taaaggacta actccaagta aaagtgaagt agactcaaaa tatcaagaac tagaagataa
     1441 gatgaatttg aatacaagct ataaggaaaa aatggacaaa gcaggtgttg ataaagaata
     1501 tttaaaacaa gaaatatcta gagatttagc aatagataaa aataaaaagg cctttgaaga
     1561 tagaataaac ataagtgata atgatatgga agcctactat acaagtcata aaaaagattt
     1621 taatgtagaa gaagtaagtg cttctcagat acttatatcg actttagata aaaataaaaa
     1681 agaagtaagc aaagataaaa aagaagcttt gaagaagaag gcagataata tattgactaa
     1741 aatcaaaaat ggagaaagtt ttgaaagttt agctaaaaaa tattcggatg ataaagcaac
     1801 tggaaaaaat ggaggacagc taggatattt tacaaaggat gataaaaatg ctgagtttac
     1861 aaaggaagtg ttcaaattaa agaaaaatga agtttcaaat gtgtttgaaa ctagttatgg
     1921 atatcatata gtcaaagtta ctgataaaag agaaagacaa aaaagtttta atgagtgtca
     1981 gagtttaata agagaatcta ttttaaatga aaagtatatt gagcatataa aaaaactgaa
     2041 tgaagatgct aaaatagaca gatagaattt ctaacagctc aatcctagcc tgtaatcgga
     2101 gggagatttt acaggaatgt ttaagagttt aaagagaacg aaagttgaga aatacattat
     2161 cgataataaa gactcttttt atagaatagc atatagttat acgaagaatg aagaagatgc
     2221 cttagatgtg gttcaagaag ccatgtataa ggcactatac tcagtggaaa atattaaaga
     2281 agtaaattat ataaagacat ggttttataa aattttagtt agaacgagta tagattttat
     2341 tagaaaaaac cgtaaatata ataatatgac agatattgac cttatagatg agactggaga
     2401 gtatgataaa tatactgatt tggatttaag aagagctttg gaagaattac ctattgaata
     2461 caaatctatt ataatattaa gattttttga agatttaaag attgaagaag tagcaataat
     2521 attggatgaa aatgtgaata ctgttaaaac taggttatat actgctttaa aaaaactaaa
     2581 attaaaaatt gaagagtagg gataaaaaga atgacaagca aagaaaggtt aaataagtta
     2641 aaagatgagt atcataaaat tccaataccc aagaaattag atactataat aaatcatgaa
     2701 aaaatagaaa acatatatcg agagaaaaat aaaaaagtaa atagattaag atttaaagtt
     2761 gcaatagcct ttgcatgtat atttacagta ttagtaaata taagtcctgt atttgcagac
     2821 aatttttcta agatacctgt aattggagta atagtagaag taataactat aaaaaattat
     2881 agtttaaaaa gtgaaaatta tgaagctgag attgatattc ctaaaatacg aggtttaaaa
     2941 gataaaaact tagaacaaag attaaacagt agttttatgg aagatggaaa aaggctttat
     3001 catcaattcc aagagagaat ggagaagata caatcatcaa agaataaagg atataaatct
     3061 ttaagtttga gttatagtgt aaaaaataat tctaaaaaat ttctatcaat agagatgaca
     3121 aaaaatgaga tagaggcttc atcatatgtg agtaaggcac attatactat tgataaaaaa
     3181 agacaaatag ttttgacttt gccaatgctt tttaaagatg ataagtatat aaaagttata
     3241 agtgataata taaaagaaca aatgagagaa caaatgaaaa aggatagtac aaaatcctat
     3301 tttatagatc aaaaaaaaga cttgcctgtt gaagatttta aaacaattaa taagtatcag
     3361 gatttttatt ttaataaaaa tgaagacctt gtaatttgtt ttgatgaata tgaagtagca
     3421 cctggttata tgggtgcagt tgagtttgta ataccatata aagtaataaa agatttgtaa
     3481 attgaatagt agtttatata tacatatttt aagaaggaca atatgactac atataaaaag
     3541 cttaaaaaat ctatatttaa aatttctata ttttcaatat gcttattagt ctcattagta
     3601 gatataaacc ctatatttgc aggtagttcc tctaatgaga cttcaaatag tgatacgata
     3661 aaggtgttaa atgctaagag tctcaacttt aaacaaggca attatgaagt gaatgtgagt
     3721 attcctaaaa taaaaggttt aaaagataaa actttagaga ataaattaaa caatgagttt
     3781 ttgcaagatg ggaaaaaaat ttacagtgaa tttcaaaata aaatgaaaaa ggaaaataat
     3841 aagggtcata gatacctaaa tgtaatccat aatgtcaaaa ataattctaa ggacttttta
     3901 tcaatagaaa tgacaaaaga agagatagaa gattcctcgt ctataactaa gaagcattac
     3961 actgttgata aaaatagagg aatactatta aatttaccaa tattttttaa agatagtaga
     4021 tatattagag ctataagtga taatataaaa gagcagatga gaaatcaaat taagaaagat
     4081 gatacaaaat cttattttat agaccaagat aaagacttcc cagtgaaaga ttttgaatct
     4141 ataaataagt atcaagattt ttattttaat aaaaatggaa atcttgtgat tagttttgaa
     4201 gaatatgaag tagtacctgg ttatatggga gcagtagaat ttgtgatacc aaataaagta
     4261 ataaaaagac taagatagat atatagaaga tttttagata gactggtaag gagaattata
     4321 tgaatatata tgaacaagca ataagattaa tagaaaataa tgaagatttc gcatttgcta
     4381 caataacgtc tcattcaggt tcaacaccac gtgaaactgg agcaatgatg attgtgaaaa
     4441 atgattctac tatatttggt tcagtaggag gaggtagtgt agaagcagaa tgtataaaac
     4501 atgcaattaa tgttataaaa aatagagaat caatgttgta taaatttact ctaaacaaat
     4561 ctgatgtggc aaaactagga atgatatgtg gaggtactgg agaaatacaa atagatttta
     4621 tagatagtaa attaaaaagt aatatagaaa aattcaataa gagactaaag gaaaatacaa
     4681 gtaaagcata tatatttggt gcaggtcata tttcaagaga tgttgctgtg atactatctc
     4741 ttttagaatt tagaacagta gtaatagatg atagagaaga atttgctaat tatgaaaggt
     4801 tcccagaatc agaagtagta gttttagatt cttttgaaaa tataccagat tttccaactg
     4861 acgaaaatag ctatataatt attttaacaa gaggtcatct atatgattca agtgctttag
     4921 aatgggctct taaaagagaa actggttata taggtatgat tggaagtaga acaaaaattg
     4981 gattgacgta cgaaaaactc atgaaaaaag gttttaagaa ggaagaatta tcaaaagtac
     5041 atgcaccaat aggcataaaa ttgaatgctc aaacaccagc tgaaatagct gtatgtatag
     5101 ctgctgaact tataaattgt agagctaata aggaaaaata aactaatgaa cacagggagt
     5161 aaagatattg atactttaaa tgttaatttt agagggattg aaataaaagc agttttagta
     5221 tataaaaata gaaaaaatat ttctataaaa atagatccat ttggcaaaat aattgtaatg
     5281 agtcctccta aaatatccaa aaaaataata aaagatataa ttattgaaaa aggagactgg
     5341 atacttaaaa agtcagaaaa atataaagga agagaagaag tatacaaaca aagaatgttt
     5401 ataactggcg aaaaatttct ttatttaggt gaagagtacc atctagttat aaaagaactg
     5461 ttgaaagatt ctgtcaaaaa aagtaactgt agaataacta ttaacgaata tcaaatagtt
     5521 gttcagacaa atgatacaag tacagatttt attaaaaaat ctttaaaatc ttggtataag
     5581 atggaaagtg aaagaacagt attagagaga atagattttc ttaaattaaa ttgtgaaata
     5641 atgaagcaat tagttccagc aagtgttaag gtaaaagaac aaaataagag atggggaagt
     5701 tgtacagctc aaaaaaatat ttatataaac tcaaaaatat ctatggcaag attagatgta
     5761 attgattatg taataataca tgagtttagc catttagtac atatgaatca ctctaaaaaa
     5821 ttctatgatt tagtaaaatc cattatgccc gattataaag ataaagaaaa ttggctaaaa
     5881 ataaatggat ataaaataat aatctgaaaa cgattattcc aaaatacagg cgtaaagtat
     5941 aaaagaagta ttattgtgaa aaaaataaat aatctgaaaa ttttaaaaaa agtcattgac
     6001 attgtaaatg atagggaata taatctatat taacaacaaa acagaattaa aatataaaat
     6061 taaaaaatta aaagcaatga cagagacaac aatactaata gtgttatcca cagagaacca
     6121 atgttgctga gaattggtgt taacagtagt ataatgatca ctctggagct tttgtactga
     6181 atatttttag taagtacaaa cgtatgtcaa cgttacatga ctaaggttaa ggtaattaat
     6241 tacccgacct gaaaaaagag gggtagatgt attttttata tctacaaata aggtggtacc
     6301 gcggaatata actttcgtcc ttataagtaa tatttatatt gcttataaag atgaaagttt
     6361 ttttatttta agcgatataa ataaatttaa tccatataaa attaataaaa aaataataaa
     6421 aataaggagg gatttttatg gaaaatactg tatatgctaa attaaaccaa ggaatagatt
     6481 cttttttaag agtggctatg actctaagaa gaagagaggt tgatatacaa tcaataagta
     6541 tgactgtaga taatatgaat aattcaggta taaaattaat agtaaatgaa gaaaagacat
     6601 ctttagattc tgtcttaaac catatgaaaa aactacatga tataagagag ataactgttg
     6661 aaagaatgag acattaatta aatatatttt ataaagctaa
//
LOCUS       DAMJVX010000177         6644 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMJVX010000177 DAMJVX010000000
VERSION     DAMJVX010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.183"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(58..666)
                     /locus_tag="PC407_003128"
     CDS             complement(58..666)
                     /locus_tag="PC407_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDF2498538.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(775..3513)
                     /locus_tag="PC407_003129"
     CDS             complement(775..3513)
                     /locus_tag="PC407_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HDF2498539.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNRKFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3661..4452)
                     /locus_tag="PC407_003130"
     CDS             complement(3661..4452)
                     /locus_tag="PC407_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2498540.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4488..5144)
                     /locus_tag="PC407_003131"
     CDS             complement(4488..5144)
                     /locus_tag="PC407_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2498541.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5137..6102)
                     /locus_tag="PC407_003132"
     CDS             complement(5137..6102)
                     /locus_tag="PC407_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDF2498542.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEHVKQYLTSKGIRWEEMINE"
     regulatory      complement(6208..6314)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         2117 a         1116 c          604 g         2807 t
ORIGIN      
        1 aagatacata cctataaaat accttcctat agatacatat cttacctata taaaaactta
       61 ttctatataa aaacgccata aaaaatcttt tgcttcttct gcataatcta tatttatacg
      121 tttactagtt tttatatcta actcatcttt actaaatact ttttttcctt tatcatcata
      181 ataaaaatca cttatatata attcttcacc cattatactc ttactattta gacttctatc
      241 tatttttaaa gctttgcaaa gttttcctgg tccgttagta atatttttta cttgatattt
      301 actcaactca gtgtaagctt tgttgtatct attcatagat atctcatcta aagaagttat
      361 aggctctacc cctcttatta atacacactc tggcacattt tctatatttg cagataaatt
      421 taagcaatta tacataccat aaattaaata aacatatata tgtccaccat ctaaatataa
      481 tggcttagtt ctatctgtct ttttattgcc atatacatgt gctcctttat catttacacc
      541 catgtatgct tctgtctcta tgattttggt aacaattact ttattttcat atttccttat
      601 tagatattta cctaatattg atttggctaa atcaattcca ttttttctaa aaaaatcctt
      661 ttccaaaatt tcacctcata aaatttattt aaaacgcatc aaatacaact aaagtaataa
      721 ataaaattaa ctcttattaa tgtgatatat ataaacaata aatttagcca tttattattt
      781 atatatatca aaaaattcta tctcaaactt ttcaatatta tcattgtaat ttgaattatc
      841 atatacttct tcaacaacct ctttaattgg gaatataact tttacattag ttccttctcc
      901 taccttacta tctatgtaaa tcttcccatt ttgaatttca atcaatgatt ttgtaaggca
      961 taaacctata ccacttccaa attcatttga tataccactg ttttcaattt gtttaaatcg
     1021 attaaatata tcatttaatt tttctttgtc tatgcccact ccagtatctt ttataattat
     1081 ctctactgtt tcgtcttttt tatatatacc tacaaggatt tctcctcctt ctaaagtaaa
     1141 ttttatagca tttgataata tatttagtat tattctttct atagattctg ggtcaagccc
     1201 taccataaca gtttcttcat ccgtatcaaa aattaaatct attcctttag acttagcata
     1261 actactaata gatgtaacta ttctttctgt tatgtcaata atatcaaaat taactgcttt
     1321 aatcttatat atgtctgact taacttgaga aatatttatt atattatcta ttaatctcaa
     1381 taatctaaac atattttgtt tagttatttt tatatgttct tttagtatat cctgaaaatc
     1441 aattattttt ccttttttat aaaggttttc aataagttga actgaactat atatattatt
     1501 taaaggtgtc ttaagttcat gagatatatt agcataaaac tccatcttca tttggtcata
     1561 ctgtctttgt tttattaatg cttttttaag tctttcagac tctctttgca cacttaaatc
     1621 tttaataatt ctcactctac ttcttttctg tccaaagtat aatgttcttg atatcatttt
     1681 tacttcttta atgtgattat tttcatttac tatatggata ctcttctctc tttctcctat
     1741 atttaaattg tttatgtatt tttttactaa tatatttcta tatttttgtg ttgtaactcc
     1801 taatacatca ctagaaattc cgaacaactc tctaaaggct ctatttgtat atagtactct
     1861 tgatgtactt aaatcctcaa gaaaaatagc ctctggtata acatcaagta atttttcata
     1921 tattattttt tgttcattta ctgttctaag aatatttatc tctttagtta tatctctaaa
     1981 tgtaagcaat ttaaagagtt tattatctga ccaatagtct ccaactctaa cttcttcaac
     2041 atgttcttca ccttctgagt cagacatcac aaaattaact atacagtttg ctgtgtcatt
     2101 gtaacttact atttcagctt tcttttcttg tgttagattt aatcttttaa atttcctatt
     2161 gtcaaagaaa tctatgttaa aagtttcaaa atattgttct aatgtcatat cgactaaatc
     2221 acttgaaaca catttaaaac tcttttcata ttttttattt atacttatta ccttattctt
     2281 ctttatgcca ccttcagcac ttcttacaag tataggagat ggaatattta gtataatgtt
     2341 ttcatagtct ttatttatat ttcttatttc ttcatctatt ttgatttcat ttgtaatatc
     2401 atttaaactt attaagtaat tgctttcatc tttattatta cttaaactta acaaataaat
     2461 tttatcattt aactctacca gtttatcaat tatacttata tcgttatcaa atttcttcaa
     2521 tttttcagca actttttcat cttcctttat aaaagttaat attttataat aattattcat
     2581 atcctttgaa aattcatctt taaatttatt atttgtgtaa cttatattaa aatcatagtc
     2641 tgttactatc catacatttg gtattgtatc cagagcttgt ctaagtaatt cttttctctg
     2701 gtctatgtag ttttttaatg ttctttgtat aatcatatcc ttttctattg ctaaatttat
     2761 ctttcctaat tgctctgact ttttacttaa ttttttattg attgaatcaa gttcataata
     2821 tggatacact actctattat taagactgta tacatataca taataagtat ggttacataa
     2881 tagtaaatat attactccta ttaatctatc tgaatctact atattaatct tagaaattgc
     2941 tacaaaacat ataatagttg taaaaaaaac aaaatatgtt aaatctttta ttaacttata
     3001 gtgctcaatg ttactatcaa gtttattcaa caaatatagt aaattaaaca tagatataca
     3061 aatattaatt aatataataa tcatattcca gttaatatca ctaaaaatat ttcttataaa
     3121 gtaatctaat atcacaaaag cactataaaa aattagtatt gggcttattc ttagtttttt
     3181 tactttagaa atggttaagt atataaaagt agacatatta attatgctta agttaagcaa
     3241 tacaatttgg aatttttgaa tttttaaatt tactactcct aaaatactta caaaaactaa
     3301 tacaagacac cctactttca atatatttat aaattcatct gttttaaagc tacttatata
     3361 aactatagaa tatccaaata tccaaagtaa aattattcta tacatattta tagaaactgt
     3421 atacaaattt atatttaaaa gtgcaataac caaaaataac ccattagcta gtatatagac
     3481 cttattctta cttaaagttc ctttagattc catattcccc accacttctt catttttata
     3541 tagttttacc tataaatatg ttaccacaat tcttccaaaa aatttgtcac tttctagtta
     3601 taaaaaaaaa tgagtaaata tttttaattt tactcatttt gtagattttg tatattcagt
     3661 ttaaaaagct ggaactatac ttcctttata ttcatcttgt ataaattttt taacttcttt
     3721 actattcata gcctctgata aagccttaat cttatcacta tctttattat tttctctaca
     3781 agctattata tttacatatg gagaatcact tgattctatt actattgcat ccttagtagg
     3841 atttaagtta gctgttaaag cataattaga atttattact gctccgtcaa catcttttaa
     3901 tactgttggt aattgctctg cattcatttc ttttattact atatttttag gatttttagt
     3961 tatatctttt ttagttataa gctctccatc ttttacttct atcaatttat tttttgctaa
     4021 taatttaagt gctctagctc cattagttgc atcatttggc acagctataa cagctccatc
     4081 ttttaattca tctaatgact taactttttc tgaatagaat cccattggct ctatatgaac
     4141 tttagatgta taagtaagtt tatatccttt ctccttcaca gtttcctcta aaaatggaat
     4201 atgttgaaag aagttagcat ccaaagaccc ttcatctaaa gctgtatttg gaagtacata
     4261 atcatcaaat atttttactt ctaaatcata tcctttttct tttaacaaag gttttgcaac
     4321 ttccaatatc tttgcatgtg gatttgatga agctcctact agtatttttt tatcctcttt
     4381 gtttgaacaa cctactgctg atattgctat tgctgacacc aatgctactg ataataattt
     4441 ctttaatttc atttttactc cccctcttta aaatgtttat aattaattta ttttagtttc
     4501 ttataagcca gatttcctac tccctggatt atctgaacta atattataag tgctattaca
     4561 gtatatacca ttactgctgt atcaaatctt tgatatccat aaataagtgc tatattacct
     4621 aatcctccag ctcctactgc acctgccata gctgtataac caagtataga tattattgat
     4681 aatgtaattc cagatactat tgaaggcata gcttccttta tcataacttt aaatactatt
     4741 tgtctttttg tagctccaaa agattttgct gcttctatca atcctttatc aacttcattt
     4801 aatgagcttt ctataattct agcgataaac ggtgctgccc caatagttat tggcactatt
     4861 gcagcagttt cccctatact tgttccaacg attaatctag ttattggaat cattgcaact
     4921 ataagtatca taaatgggaa acttctaaat atatttacta taaatcctaa tgcactattt
     4981 attgtgctat ttggttttaa tccatctggt tttgtaacta ctaatatgat tgctaatata
     5041 aatcctaata ttgttgccac tatagttggt actataacca tatacaaagt ttgtaacaat
     5101 gcatttggaa atagtgttgt taaaaagtca attaaactat tcatttatca tttcctccca
     5161 tcttatgccc ttactagtta gatattgttt tacgtgctct ccacttttat ctgaaatgtt
     5221 tataataagt gaacctaaaa tatcatcttt aaattgttct aatttcccaa atattataga
     5281 cacatctata tttagttctc ttgccatagt cgtaataata gcttcattag aaatatcttt
     5341 agggaatagt atctttatat ttgttccttt tggcaatatt atactttctt cacctattaa
     5401 ttttctaagt cctttagtat ttcttaagaa tatttcttct gtatctccta cctctaatac
     5461 ttctccattt tccattattg ctactcttcc acaaatctgc tttataactt ccatttgatg
     5521 agttacaact attattgtta ttcccaattt tttatttata tcttcaagca atgaaagaat
     5581 tgactttgta gtatttgggt ctaatgctga agttgcttca tcacaaagaa gtacttgtgg
     5641 gtttagtgct aaagctctag caattgcaac cctttgtttt tgtccgccac ttagatttct
     5701 gggtttatca tttttctttt cagatattcc aactacttct aataactcta aaactctttt
     5761 tttaatttct gcctttgagt atccaaaaca ttctaatggc aatgctacat tatcaaatac
     5821 agtttttctt tcaagcaatg aaaagtgttg aaaaatcatt cctaactctt ttcttaaatc
     5881 tctcatttgc ttttcattca atgactttac ttctttatca gaaacaagta cactgccttc
     5941 ttggtattct tctaatccgt ttatacatct cagtagtgtt gatttccctg caccactatg
     6001 acctattatt ccaaaaattt ctccagattc tatttcaata cttacatctt taagcacttg
     6061 aatctttcca taatacttat taacattttt tatgctaatc ataaaatttc ctcccatata
     6121 ttctgctcag tttttatgtt tatcctctct tatgtattac aacaaaataa aagcccgagt
     6181 ataaactcag gcttagaaag ctatttatac tcatctatca actatattag ttgcaggatt
     6241 tagcaccaat gtatataaaa tatactggtt gccgggtttc aaagggccag tccctccacc
     6301 actcttgata agacgttatt taattcaatt ctattatcat ttgttgctat gttatatatg
     6361 atatattcat taatagtatt tgtcaataaa tttatttaaa ttttttattt tttattatcg
     6421 ttaattaact atttttaatt agttttctag ctcctaacac tttatatttt aatgtctata
     6481 taactataga acatcattat aagaaataaa ttaaataaca tcaatctatc taaatcatct
     6541 gtaaagtaaa ttaagaaaat taacatttta tccaaactct atcctactat taaataaaat
     6601 aatttaaata caatgatgat tatatagctc taatacttta gact
//
LOCUS       DAMJVX010000178         6615 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMJVX010000178 DAMJVX010000000
VERSION     DAMJVX010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.184"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(529..1197)
                     /locus_tag="PC407_003133"
     CDS             complement(529..1197)
                     /locus_tag="PC407_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2498543.1"
                     /translation="MPVLMAIGVPVHLAIGTNKFAASAGCISSAYRYAKSGKINNDLL
                     KKLVPFTIIGSVLGVRCVLSISEEILNVLVVVMILIVAIYTFISKNLGQEDNFEAVNK
                     KNLRLGMLMALIMGFYDGFFGPGTGTFLTFGFIKIYGYDFLHASANTKILNLTSNITS
                     LLLFMINGQVDYKIAIVFALVMIMGAYVGAKVAIKKGSKMIKPIFLVMALFMVVKLVY
                     QTLV"
     gene            complement(1142..1282)
                     /locus_tag="PC407_003134"
     CDS             complement(1142..1282)
                     /locus_tag="PC407_003134"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498544.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGTYKHACSYGNWSASTSCHW
                     YK"
     gene            complement(1341..2996)
                     /locus_tag="PC407_003135"
     CDS             complement(1341..2996)
                     /locus_tag="PC407_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2498545.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQMIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKGI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVDSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(3225..3593)
                     /locus_tag="PC407_003136"
     CDS             complement(3225..3593)
                     /locus_tag="PC407_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2498546.1"
                     /translation="MKYSITDVSKITKISSSAIRFYEEKGLLLPINRKPSGVRYFSEE
                     DVERLIFIKELKNTGLSIKDIHECFHFCDMGDGTIDKRIQLFQQHKQNIKNKIHELES
                     CMDKIDEKIYYLKEKKMCKK"
     gene            3742..4536
                     /locus_tag="PC407_003137"
     CDS             3742..4536
                     /locus_tag="PC407_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase"
                     /protein_id="HDF2498547.1"
                     /translation="MEKYATMIKEKLNEGKVLCGVAVSLSDSVVSELIGISGYDFIWI
                     EGEHSALDRKDIQQHMIAAHAGGAASLVRLVNSDPDLVKPIMDMGTDIICFPFINTVE
                     DAQKAVAACTYPPNGIRGYNPQRASYYGNMNYFDYVKNANNETLKMMLIEQKAGYDNL
                     EEIVKVEGIDIIALGPGDLSLDMGFSGYMNRIEIKDMISHAAEICKKYKMPFIIFPTI
                     EKKSIEEWVNIGANMLCFAQDTTFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(4689..5117)
                     /locus_tag="PC407_003138"
     CDS             complement(4689..5117)
                     /locus_tag="PC407_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2498548.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(5593..6531)
                     /locus_tag="PC407_003139"
     CDS             complement(5593..6531)
                     /locus_tag="PC407_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2498549.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         2230 a         1104 c          655 g         2626 t
ORIGIN      
        1 taatttagca taaccttgtt aaatatagat ttgaatatga atttaaagtt atgtaaaatt
       61 aaaaatacag gaatgaaaat tccattcctg tataaaatct aacttttgag tctcaataca
      121 atcttatttt gagacaatct tttttgtttt atattttctt atatttaaaa tataacgcat
      181 atcttatgca acatattcat atttttcttt atattctttc atcgcatcat tccaagcgtc
      241 ttgtactgtt acacctcttt tattagcaat tttttgtgcc agttcaatta tcttcatagc
      301 tcttcttgat atgttcataa acattcctcc tacaaattac catatatttc atatatataa
      361 atcaaggtta tatttttatg atatataatc cttctattgt aatattacaa tttatatgtg
      421 tattatatat gaactatatt agaagataat tagaattttt ataaagctat atgattagat
      481 taaatataaa agctttacaa ttacttataa atttaaaaag tctataaact aaactaatgt
      541 ttgatatacc aattttacga ccataaataa tgccattact aaaaatattg gttttatcat
      601 ctttgaacct ttctttatag caactttagc acctacataa gctcccatta tcatcactaa
      661 tgcaaacact atagcaattt tataatctac ttgaccattt atcatgaata ataacaatga
      721 agttatatta cttgttaagt ttaatatttt tgtatttgca gaagcatgta aaaaatcata
      781 tccatatatt tttataaatc caaaagtcaa aaaagttcct gtacctggac caaaaaaacc
      841 atcataaaaa cccattataa gagccataag catccctagt cttaaatttt tcttattaac
      901 agcttcaaaa ttatcttctt gacctaaatt tttagaaata aaggtgtaaa tagccactat
      961 taaaatcata accacaacca aaacatttaa tatctcttca cttattgata atacacatct
     1021 cacacctaga acactaccaa taatagtaaa tggtactaat ttttttaaaa gatcgttatt
     1081 aatttttcca gactttgcat atctataggc actacttata catcctgctg atgctgcaaa
     1141 tttatttgta ccaatggcaa gatgtactgg cactccaatt gccataagaa caggcatgct
     1201 tataagtccc tcctcctcct gctatagaat ctacaaaagc agctaaaaac ccccctatac
     1261 ataaaaaaat aactgaaaac ataaaattcc tccatcattc tctttcaatt tttcttacaa
     1321 tatatattat ataatcttat ttaatactta ttatccagca tattctttct atattcattt
     1381 ggagttatac tattaaactt cttaaatatt tgtgaaaaat aactactgtt attaaaacct
     1441 actttcttac aaatatcact aatactcatg cttctatcta acagatattt tttagattct
     1501 tctatcctaa gaagatttat atactcagtt attgaaactt tatttctttc attaaaaata
     1561 gaactcaaat aatttggact tataaaaaaa acatctgata tactattcaa atttatattt
     1621 tctttataat atttttttat atattcgttt atattttcaa ttagatttcc tttatctttt
     1681 ctccaaaatg ctctattctt atcaatacac tctttggaaa attcaaataa tacattccat
     1741 aaatcttcat cactgtctac aagtgatatt tttttattat atttatttct taaataagat
     1801 ataagtttta aatcctctaa atctctaagc actgttctag acaatacaaa tatcatttcc
     1861 tgaatagtat ctctaacatc attatatctt aatttgtttg tatctattag attataaaca
     1921 ccttctaaat tcatattttt aatttctttt tcaatataaa gttctaattc aaagggatat
     1981 tcttttgaag aattattgaa catatctatc ggattaataa tatactcttc ttctaataat
     2041 ctactatttg cttttttaag tgcctcttca agtcttatat tttcttttct actctttaat
     2101 atctcctcat cttttatact aagttgattt tcaaaaaaat tcctcttagc tatttcctga
     2161 ataaattcag acatcattaa taaattttct tttaatacat ataatctact tttaggattt
     2221 aatggaaatt cattgtaaat tttgtttatg tattttatat caataccaga actacttgat
     2281 aattcagata aatttttatc tatatcaata gacttatata ctataaaatt accacattct
     2341 atatatccaa ctatattttc ttctaatact ataggtatct ctaaactgga aacaccatac
     2401 tcacaatggt acactttttc aaagttatca ttatttatgc ttttaacaag ttttttagta
     2461 cacggtttta tatttttaca aatatcgcaa aatttatttt ctttcttagt ctcaaatatt
     2521 cctttatact ttaagtcata tacagatatt ctaagcttca atatattaga tatttgattt
     2581 gcaagatttt caattatgtt tacattaact aactcttgta tattgttttc agaaaatact
     2641 ataggtattt gtctcaaact cgaaaattta tgaggaacat aagtaattaa tattactaaa
     2701 tcaattttac cagtgtttat aatttcatgt tctgaatatg gtgggcaata aactatagat
     2761 gtttccttta tattgacctt tttaccatca atcatttgta ttccttctcc ctgaatcaca
     2821 tataaaagct gttcttcact taaatgaaaa tgcttttctt ggcagtttcc cggaaaaaat
     2881 cttacaattc cagcactaag tctattgaat tgtgtttttg atggttcgtg tatccacata
     2941 acctctcccc aatcaaattt ttgtacttca ttatttgact caataaaatc caccataaac
     3001 tcacctcgta gtttttatct aaatattttt ttattccttt aaatatctcc ttataatata
     3061 cattatcata attttctttt ttattgtata gataaatagt tatatagaac attgtccaat
     3121 tcttcctaaa aatatagtgt aaaaaatctt tcctcaaatg tttccaattg aatttactct
     3181 ttatattaga aaaatcaaaa aattatttct ttattctata aggattattt tttacacatc
     3241 tttttttctt ttaagtaata tattttctca tctattttat ccatacaact ttctagttca
     3301 tgtattttat ttttaatgtt ttgcttatgt tgttgaaata attggattct tttatctatt
     3361 gtaccatctc ccatatcaca aaaatgaaag cattcatgga tatctttaat agataaacca
     3421 gtatttttta attctttgat aaatattaga cgctctacat cttcttcaga gaaatagcga
     3481 acaccacttg gttttctatt gataggaagt aataaacctt tttcttcata aaatcgaata
     3541 gcagatgaag aaatttttgt tatttttgat acatcagtta tagaatattt cataatctct
     3601 acctccctaa attttatatg aataagaata ttataaaaat accttataca gtagtcaaca
     3661 aaaaagtatt gacttcaagt taggttgaag ttgtactctt tgaagtgtaa aagaaaactt
     3721 acaaaagaaa ggatgatata aatggaaaaa tatgcaacta tgattaaaga aaagctaaat
     3781 gaaggaaagg tcttatgtgg agtagctgtt tcactgtctg attcagttgt aagtgagtta
     3841 atcggtatat ctggatatga ttttatttgg attgaaggag aacatagtgc tttagaccgt
     3901 aaagacattc agcaacatat gatagctgcc catgctggtg gtgctgcttc tttggtacga
     3961 ttagtaaaca gtgaccctga ccttgttaag cccataatgg atatgggaac agatattatt
     4021 tgtttcccat ttattaatac agtagaagat gcacaaaaag ccgtagcagc ttgtacttat
     4081 cctccaaacg gtataagagg atataatcct caaagagcct cttattatgg gaatatgaat
     4141 tattttgact atgtcaaaaa cgcaaacaat gaaacattaa aaatgatgtt aattgaacag
     4201 aaagcaggtt atgataatct tgaagaaata gtaaaagtcg agggtattga tattatagca
     4261 ttaggccctg gtgacttatc tttagacatg ggtttttccg gatatatgaa tagaattgaa
     4321 ataaaagata tgatttctca tgctgctgaa atatgcaaaa aatataagat gccctttatt
     4381 atttttccta caatagaaaa gaaaagtatt gaagaatggg ttaatattgg agcaaatatg
     4441 ctttgttttg cacaagatac tacctttatc acttatgtta tcaatcaatt attatctttt
     4501 tataatgaga ttgtaccaat tcaaaaacaa cattaaatta taaatctaat tttatattaa
     4561 aagagactaa tattttatct atctctatta aggatatttt cttatgtatc ataatattag
     4621 tctctcttat tattatatac tgctatcatc attagttgct atttatacta tttttataat
     4681 atacaaattt attcagctaa cgttttgcct aattctatac actcatcttt tgcttcttca
     4741 tttgggtctt ccattgctgt aattccttct ccaccaataa ttgtagcacc agcattttgc
     4801 atacgttctt cccagtctct catccattca caatttcccc atccatatga accaaataat
     4861 ccaatctgtt tacctgatac catagattct aattctgaaa caaatggctc catatacccc
     4921 tcttctagtt gctctgctcc catagatggg caacctaatg caaatttatc ttcttctttt
     4981 aaatcacttg gtttcagtaa gctcacatct aaaacttctg gtgtcttacc ttttaacttc
     5041 acaccttcag ccacaaaatt tgccattttt tcagtatttc ctgttccact ccaataaaca
     5101 atatatattt tactcatttt tacctcctgt agtatttttt aattaatcaa tagttagtta
     5161 tagctaacta acatctaaaa aacaaaagat ttctttatct gaaatctctt tatttttcta
     5221 aatatagaat atcacaattg attttgaaat tcaatattta ttgataatat ttatcattta
     5281 tattttatat tcattttata atatcaaaac actgtatcgt atttctaatc tttcatagaa
     5341 aaatttctaa cttatagtac ttttgcatct cttataaatc ttttatatgt ataagtacat
     5401 agaaaaacac accttttcca gatgtgctat ctataatatc gtatatttat ttttcaattc
     5461 taactagcta atatgtttat aaattttatc acttaatacc tttatacatt gatgtaaaat
     5521 cttacaccta agtaattttt attatttgtc tcgactctat ataatatagc tattttatca
     5581 tcaaatcaaa aattatcttt tgctattatt cttgtattta taaagattta aatataagta
     5641 tccatttcct tgttccgttc taggtattcc aagcgctctc gtacatttta ttaattgtgc
     5701 atacagttca gcttcatcta aatctctttc aaactcctct cttgcccatt ctttaattag
     5761 tgctaatgag cctgatacgt atggtgcact catactagta ccactcataa tagcaagttt
     5821 attatccata taagtcgata taatatttct tcctggagcc accaaatcta ttgtagtatt
     5881 tgaattacta aacttttcaa ccaaatagtt ttcatttatt gcacctactt ctattacctc
     5941 agcataactg gctggataac tatactcact tgtactagaa tcaccatctc cattattacc
     6001 tgctgcacac actacagaaa tattattttt tactgcttgc atgacagcat tttttaaatt
     6061 cttatcatct ttgtttcccc caagagacat agatataata tcaaccttgt tatttacagc
     6121 aaagttaata gcattaatta tactttgata tgttccggta ccatctttat ttaatgcttt
     6181 tgctattaat aatttacaat ctggagctac tcccataact tcattattat agttagatgc
     6241 agctataata cccgccacat gagttccatg gccattaaaa tcctcatata tatttttatt
     6301 tccattactg tcatcactaa aatttgcacc accaattatt tttcctttta aaagaggatg
     6361 agatatatca caacctgtat ctattatacc aactacaata tttttaccag tataaccttc
     6421 atcccacatt cccctagcat tcatttgttt tattccatat gggaatttat tttttgcacc
     6481 tcttagattt tcatttattt catacggaat tagtttcatt cttttattca tcatatatcc
     6541 tcctaaaaat tttattgctt ataaaaatca tgaattttat tttataatag gtttataaat
     6601 caaattaaaa cctat
//
LOCUS       DAMJVX010000179         6603 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMJVX010000179 DAMJVX010000000
VERSION     DAMJVX010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.185"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            9..911
                     /locus_tag="PC407_003140"
     CDS             9..911
                     /locus_tag="PC407_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2498550.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIATF
                     GKLLIPFIMAGILGIVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTSHIF"
     gene            1520..2533
                     /locus_tag="PC407_003141"
     CDS             1520..2533
                     /locus_tag="PC407_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2498551.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKTFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPCDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFEIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVY
                     IDN"
     gene            2889..3458
                     /locus_tag="PC407_003142"
     CDS             2889..3458
                     /locus_tag="PC407_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2498552.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKSIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            complement(3795..5132)
                     /locus_tag="PC407_003143"
     CDS             complement(3795..5132)
                     /locus_tag="PC407_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2498553.1"
                     /translation="MDTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTENEFSLFLYIRLF"
     gene            complement(5572..6282)
                     /locus_tag="PC407_003144"
     CDS             complement(5572..6282)
                     /locus_tag="PC407_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2498554.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELEKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVHIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLEP"
BASE COUNT         2446 a          693 c         1000 g         2464 t
ORIGIN      
        1 ttggaaatgt gttgccacaa gattattatg gattaatagt gattttaggt gttattcttg
       61 gagttatggg ggcattttac aactggttta ctctatatgt gcagtcttta tacaagaaga
      121 ttcctaaaat tgctaccttt ggaaaactgt tgattccatt tataatggct ggtattcttg
      181 ggattgttat gcctgatgtt ctggggagtg gacacaattt agttgaagaa cttacaagtc
      241 atgaatatat ttttggaatg gttgttttga tttttgttat acgttttata ttttcggctg
      301 ttagttttgg gtcaggtgca ccagggggaa tattttttcc attgcttgtt ttaggagcat
      361 ttataggtgg aatatttggt atggtaggtg ttaaagtttt tggaatgaat cccgattatg
      421 taaataactt tgtgcttttg gctatggcag gatattttac agctattgtg cgtgcccctt
      481 tgactggtat catattaatt tttgaaatga caggttctgt tagtcaaatg ctttcactat
      541 cagtaatttc aattgtggca tatattgtgg ctacacttat gaagtctaaa cctatttatg
      601 aaagtcttct tgagaggtta cttgaaaaaa atggagaacc tgtacataaa gagaggggcg
      661 aaaagatact agaccagttc tctgttatgt ttggctctcc tgttgcgaac aaggcaattc
      721 aggatgtaca gtggcctgaa aattgtcttt tggtagcaat tcaaagaggt ggagaagaaa
      781 ttattcctag aggtaagaca attcttagac caagtgatgt aattgtgact atgacagatg
      841 agagagatag tggtgctgtg tatgataaga tggaaaattt gtgtaaagaa catacgtcac
      901 atatttttta gggataatga tgaatgttta ataatgatag aatatttaat gaagatttga
      961 ttaaaaaatt ggtaatttga aagagagtta aggaattaat agaaaatgaa aatgtgatat
     1021 cttagatgta aaagagtttg aagaaaataa ttgtatatat ttgttaatga gattatatgg
     1081 actaatggta tatttatact gtgctaaaag ttgcactatt gtatgaggaa tgggaataaa
     1141 gggtttgaaa aaataggtag aagatttttc agatattctt attgggatat tttaattaat
     1201 atattttaat tgcgatattt tgggaagaat agaatttatt tataaatgaa aataattaaa
     1261 aggggttaag gttgtattag ccccttttgc ttatagagtt tggaaatggt atcttgatag
     1321 cagttgtttt atgcttatta tattttatta catattttaa aattaacctt atcactccta
     1381 aatcattatt ctatcaactt ttaaaacact ttcaagacaa tcacctattg aaaacaaagt
     1441 taattaaaaa cctacatatc agtttagtat tcgtgtagat gattatataa atataaaagt
     1501 ataagaaagg tgagtatctg tggttagttc atttttaaat gatatcttta gaacaatttt
     1561 agtaagttgt atttttatag ttattttatt agtctttaga ataactttat ttaagacttt
     1621 cagtaaaaaa tttaattact atatatggct tattgtgatt attaagctat cattaccatt
     1681 tatgaattat acttttatat ttaatgagtc aaaatataat ggtaatatta ataagataag
     1741 tttaggcaat tttaatagta tttctattgt atatagtact gttttagtct ctgtttggat
     1801 tgctattacg atattttatt tagtttatac tttattgaaa tatattaagc ttaaaaatat
     1861 gataaatgat ttgtcttatg atgttgagga tgataagata aaaaatcttt atgaaaattt
     1921 attaaaagag cttaagataa ataaaaaaat acggcttaaa tattcatatg aagttgaaag
     1981 tccagtattt tttaattcgt gtgtcatttt accaccatgt gattatacat taaaagagtt
     2041 aaactggata tttaggcatg agttgatgca ttttaagagt aaagaccttt acattaagta
     2101 tttggtgata tttttaaaat gtgtttattg gtttaatcct tttgtgtata taatggagaa
     2161 gtttatagat ttggattgtg aattttattg tgatgaaaga gttttgaaga attgtacgat
     2221 agaagagaag caggattatg cattgacaat aattagtgca atgagaaaag gttcaaatag
     2281 ttcaaataaa tttgtggctg gattacataa ggagtcagat attaaaaaaa gagtgtttag
     2341 tatgtttaat gaaaagtaca gaaatggtat tttgatagca gttattttat gtttattatc
     2401 ttctattaca tattttgaaa ttgatttaat tactactaaa tcaatatttt atccactttc
     2461 aaaacaaaca cctattgaaa atagaactaa ttcaaaacct acatatcaga ttaatgttta
     2521 tatagataat taaaaaatac aaagtttaac aagagattaa tataaataat tttaataagt
     2581 atctttcaca tatgttaaat ttactttaat tatattaaag ccaatgaatt attctttaga
     2641 agctccaatg ccagtaaata caggtaaaga aaaatctttt actacaattg taaattaaaa
     2701 aatattctaa tatttaaaga aatgcaaaag tgtaagaagg gtaaaaatct gttataatac
     2761 attaaaaact aaatttagaa aaatttacat aattagaaaa atgatttgat aaaaaataaa
     2821 gttaaatatt aactaagtaa atttgaaaaa gaaaaaatat aaagtacaaa tttaaaagga
     2881 ggtaactaat gaaaaagaag ttggaagata tgactttaga ggagttatgg aacttgtttc
     2941 ctatattttt gactactcac aaagaatgtt ggaaaaattg gtttaaagaa gaacaatata
     3001 gactaactgg aatattgcca atgcaaaaaa ttgttagaat aagtcatatt ggaagtactt
     3061 ctgtgagtac gatttatgcc aagtccattg ttgacatact gattgagata attcctgatt
     3121 ataatatgaa agatattaaa gatttgattg agtcgaatgg atatatttgt atgtctgaga
     3181 gcagtagtag gctttcactt aataaagggt atacagtaga tgggtttgaa gaacgcgttt
     3241 ttcatttgca tataaggtat gtaggtgata atgatgagtt gttttttaga gattacttaa
     3301 ttgaccaccc agatgttgca aaggagtatg aaaaacttaa gttggacctt tggaagaagt
     3361 atgagcataa tcgtgatgct tatacagata gtaaactaga ttttgttagg gagtttactg
     3421 aaaaggcaaa ggtgttgtat tctaatagat ataattagaa ataggtacaa ttagatattt
     3481 atcttatata tcgtattata ttttttaaga aagtaattaa tagaaaaagg ggttgtttca
     3541 aaatggagag ataaatccct ttttcattgt tgaaatgata aaaatatatt tgatttcatg
     3601 taaaaaagag tgcctcatca actaaaaagt ttattttgag acacacacat ttttattaag
     3661 gtaaataaaa atatcacatt tgattaaatt ttaagttaat gaatatatgt gtaaatattt
     3721 aattataagt tattataatt ttacatagta taaaacaatc taatttgtat tatataagat
     3781 aatctaattt tagactaaaa taatctaata taaagaaata gagaaaattc attctcagta
     3841 taagagatat ccaattttcc attgtactta ttaacaattt ctctaatatt ctcaatacca
     3901 tatccgtgag aattactatc ttttttatct gaaataaata gttcttgttt tctattgata
     3961 ggatttacct tgttgtttgt caataaatag ctaaaatact ccttctcaca acgtgccttt
     4021 aataccactt ttctatttga caaatcagga attttcaaag atgcttctat agcgttatct
     4081 aaagaatttg aaaaaatagt acataaatct ataggttcta tacccaagtg attgtctata
     4141 tctatattaa agtgcacatc aacaccattt tcttccaaac ttgtatgacg aagatttaaa
     4201 attgagttaa gagcttggtt tttgcaaaat tgtttgccac taagagtacc tatacctatt
     4261 tttaactgat taaaatactt tttagctcca gcaatatctt caatttctaa atacgattca
     4321 atcatttgaa agtgattatt catatcatgt cgcattttgc gtatttgaat ttgttgattt
     4381 tctagtaagt tatagtattt ttcttttagg tgatttgctt gtttttctat cttcatcttt
     4441 acattttcaa tcaatggctg taaaagaagt atcacaccga tatttgccag aattgctgac
     4501 aagatgatta gataagtgcc ataaggtaag ttgcctaaga taatattctt attactaccc
     4561 atagggatta aattagtagg tggcagaata atagcaatta atattgataa aaatgaagat
     4621 atacatacca tatcaataaa aatccatatt ttatttgata agtattgttt tatattgacc
     4681 aatctattct ttaggaaaat atgtattaaa aaccaaaata ataatttaat tagagcagaa
     4741 attatctgga ctattgaaag tattaaaata tattcttttg agtgtataag ttcctcatat
     4801 atagggttgt ttataaaaag aaaattaagc ccaattataa taggataaag tatcatgaca
     4861 actgatatta taggaattaa tttgcttttg ctacatatta taagtagtat aaaatatccg
     4921 aatagtgtaa acaaaatatt tacggggtcg gaaagccata ttatcatttg actaataata
     4981 gagtagccca aaatcaatat gattttgaaa atccaatgat tcctaaagtc tataaaatag
     5041 gaagtcaact tataaagaaa aaaggctatg aaaatattga ttaatattag aataaaattt
     5101 atgcttgtag agattaaaga ataagtgtcc atttaatata cctttctatt aattaagatt
     5161 ctggaaacta agttttaaaa aattaaatct taaaaattaa aattctggaa attagatttc
     5221 aaagaattga ttttgaaaaa taaaattcta gaaattaagc ttcaaaaaat tgagttttga
     5281 aagattgaaa ttttaaaaat taagtttcaa aaaattaaat ttgaaacaaa actctaaaaa
     5341 ctaagcttca aaaaattaaa ttttgaaaga ttgaaattct agaaactaag ctttaaaaaa
     5401 ttaagtttca aaaaaactct aaaaactaag cttcaaaaaa ttaaattttg aaagattgaa
     5461 attctagaaa ttaagcttca aaaaattaaa tttgaaaaaa caaaactcta aaaactgagc
     5521 tttaaaaaat taagtttcaa aaataaaatt ctagagattg aacttcaaaa attaaggttc
     5581 taacatatac cttgtaaatt ttcctacaac ctcttgatat tttttacggc ttataggaat
     5641 tttttcattc tgaactttgg cataagtttg agttatacta tctacatggt ttagattgac
     5701 aatatacctt tgatgcgtac gtataaaatt agagggaaga acagtttcca tatcatttaa
     5761 ttttccgtag tattctaatg ttttatcaaa ggtaactaca gttaattttc ttagctcgct
     5821 tttaaagtag aggatatctt ttgtattaat tttacaaaga ttagttccaa tatgaactgt
     5881 aataaagttt tctttccatt gttctaattt ttttagtgct tcttttaata ctttacatat
     5941 tttttcagtt ttataaggtt ttaagatgta gttcaaagct cctacatcat acccatgaaa
     6001 tacatagtca gaaaaacctg tgacaaagat gattatagta tcttttgaat gttttcgtat
     6061 agtttttgct atgtcaacgc cattactttt ttcaagttca atatctaaaa aaataatatc
     6121 tatgtaataa ttttcgtgtt ctagattgta tactagactt tctccatttg aatattcttt
     6181 tatttggtag gggtagcccc acaaatccat ttctcttgta atgattttat tcagcatgtt
     6241 tctttgttgt ttttcatcat cacaaataca aatcgaagtc aattttttat ctcctttcaa
     6301 gttttatatt tatatagtta agtatataat atattttgct acctagatat ataaaatgaa
     6361 ataacaatta tatccagata gcaaaataaa attttttata ttagtatact tacacttaaa
     6421 actattctat taaacttatt ctgaagtgct aatgattttc ttattaaaaa atctaataac
     6481 ctttcttatt ataaaatacc aatgctttta ttattataaa aatgtaatga cttttcatat
     6541 tataaaaagc aaatgattat cttactataa aaatacaatg acttttaata ctataaaaag
     6601 caa
//
LOCUS       DAMJVX010000180         6600 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMJVX010000180 DAMJVX010000000
VERSION     DAMJVX010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.186"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            108..566
                     /locus_tag="PC407_003145"
     CDS             108..566
                     /locus_tag="PC407_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2498555.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(683..1420)
                     /locus_tag="PC407_003146"
     CDS             complement(683..1420)
                     /locus_tag="PC407_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2498556.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            1637..3313
                     /locus_tag="PC407_003147"
     CDS             1637..3313
                     /locus_tag="PC407_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDF2498557.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            3519..3719
                     /locus_tag="PC407_003148"
     CDS             3519..3719
                     /locus_tag="PC407_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HDF2498558.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            3773..4936
                     /locus_tag="PC407_003149"
     CDS             3773..4936
                     /locus_tag="PC407_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2498559.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(5147..5569)
                     /locus_tag="PC407_003150"
     CDS             complement(5147..5569)
                     /locus_tag="PC407_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HDF2498560.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(5590..6426)
                     /locus_tag="PC407_003151"
     CDS             complement(5590..6426)
                     /locus_tag="PC407_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2498561.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
BASE COUNT         2518 a          796 c         1077 g         2209 t
ORIGIN      
        1 attatgagaa taaattaaat ttatttaaat gtcttgacaa aaaatgaatt atcaattaca
       61 atattattta ataaagatta tcaattgaaa atagtaaggg tgatgttatg gcaaatacaa
      121 tggatttatt aaaagacaag ttaaaagaaa ctggttttaa gattacgcca caaagaagag
      181 caatagttga aatattgctt aaacatgacc attcacatct aagtagtgag gaaatctatg
      241 atttagttag ggttgactgt cctgaaatag gcttagctac tgtatataga acaatgcagc
      301 tattggatga aataggatta atatcaaaac taaacttaga cgatggatgt ataagatatg
      361 agattagtct acataaagaa gactgtcata atcatcatca tcttatttgt aaaaattgtg
      421 gaaagataat ggaagctaaa gaagaccttt tagacaatat agaaaaagag atacaatcat
      481 tgtacaaatt taaaatactt gatcatgatg ttaaatttta tggattatgt gacgagtgta
      541 atggagtaag cgattcagaa gaataaactt ctattgctta atgtcgatgg aagaatagta
      601 tagaactttt atattctatg ctattcttcc attttttatt ataaaccatc tacaaaatgc
      661 aaatatacag ttttcaagat gcttatagtt ttaatattga aaatataatt aatcctataa
      721 gtacaaaaat gaatgatttt tctatgaaat tactaaatgg tcctccaaca gttaaaaaac
      781 ctaaagaaat tttcttttcg cttagcggat aaaaaattgg tattccttgc tttgtaaata
      841 aatctcctaa aaacatatga cttgcataac caatcgtacc ataaaaagat aaattattaa
      901 tattagtgat taattctatc tgttttagta aaaaatatac tattatagtt gcaaatatgc
      961 tatgagaaaa acttctgtgc tttaaccagg gaaaaattga caacattaga caaaaaattg
     1021 taaaatatat ttcaacttct atgatgtata agcatatagc taatagtata aatattaatg
     1081 atagaaaaac ttttcttaga aatgtgtggg ttagttttgc ttcaataatt ataattgcaa
     1141 caaatgtaat tattgcgctt aaataaaaat tatcatttat atttattgaa ataaagaaaa
     1201 taataatatt aacagcatat ataaaaattc tatatatgta tttggataca ttgtttttta
     1261 gaatgaaatt tgagaccata gaattagatt tatccaaatc gggtagtaca gcaaaagtag
     1321 ctgaaactaa aatatcaact aaagatatag gaattttaaa tattaaagat gtttggatag
     1381 ctgttaagat tcctattgca caatgagttt taccacgcat aaatcctcct aaattatccg
     1441 atattaaatc atataatatt tagaggatat caaaataaaa taggtttgtc aaaataatac
     1501 gttaaatgta ttttaaatat agcttaaaat atgatacgaa ttatggtaca ataagaataa
     1561 ctggtagcga gtgcgtgatt tgatattatt tatgattgta gaacgtgtta aaaaattaaa
     1621 tagacagaag gagatgatgc aattgttcaa aaagaacacc aacaagataa aggtaatggc
     1681 cttgggtgga cttaacgaag ttggaaaaaa tatgactgtt gttgagtata aagatgaaat
     1741 aattgttata gatgctggac ttagttttcc agaggatgaa atgttaggag tagatatagt
     1801 tataccagat ataacttatt tagtaaaaaa tagggataaa ataaagggta tatttattac
     1861 acatggacat gaagaccata taggagctct tccatatata ttaaagaaaa taaatgtacc
     1921 tgtatatggg gcaagactta gtataggtct tatacaagta aaacttaaag aacataaaat
     1981 gaataatgtt aagttaaatg ttataggtcc tagacaagtt ataaagcttg ataatatgga
     2041 agtagagttt ttgaaaaata accatagtat accagatgct tactctattg caatacacac
     2101 agaccaaggt ataatatacc acacaggaga ttttaagatt gacttgactc ctattgatgg
     2161 agatgttatg gatatgcata gaatctgtga gttgagcaaa aaaggagttc ttttaatgct
     2221 ggcagatagt actaatgcag aaaaaccagg atttactatg tctgaaaaga ctgttggggt
     2281 aggtcttgac gaattatttg caaaaggaaa tggcagaaga attatagttg ctacctttgc
     2341 atccaacata cacagattac aacaaataat aaatactgct gaaaagttta atagaaaagt
     2401 tgctatatca ggtcgctcaa tggtcaatgt agtcggagtt gcaaaagaat taggatacct
     2461 agatatttct gatgacatgc ttatagattt aaatgatata tgtaagtatg aagatagtga
     2521 attagtgata ataacaactg gttcacaagg tgaacctatg tcagctcttg ctagaatggc
     2581 tttttcagag cacaaaaaag tagaaataaa gagtggcgat ttagttataa tatctgctca
     2641 tccaatacca ggaaatgaaa aattaatatc aagagtaatc aatttcttat ttgaaaaagg
     2701 agcagaagta gtatacagtg atatagctga tatacacgtt tctggacatg cctgtcaaga
     2761 agaattaaaa cttatacatg cattagttag accaaagttt tttatgccag ctcatggtga
     2821 atatagaatg ttaaaaagac atgctgaaat agctgaacag cttggtatgg ataaagaaaa
     2881 tatttttgtc atgcaaacag gagatgtttt agaattagat aaaaactctg ctaaggtagc
     2941 aaaccgtata caaacaggaa acatattagt tgatggtcta ggagtaggcg atgtaggtaa
     3001 tatagtttta agggatagaa agcatttatc agaagatggt cttatgatag ttgtagttac
     3061 tatttccaag gatgagggta aggtcttagc aggtccagat ataatttcca gaggattcgt
     3121 atatgtaaga gagtcagaag atttaatgga tggagcaaag gatataatta agaatgttct
     3181 aaatgaatgt gaagagaaaa atattaaaga atgggcttat ttaaagaata atattaaaga
     3241 aaacctaaaa gaatatttat atcaaaaaac taaacgaaat ccaatgatac ttcctatcat
     3301 aatggaagta taatataaaa aaactataaa tttgaaaatt agacctcttg taatttttaa
     3361 agtaatatca aaaatccaat tgatattatc ttaagaatta caagaggtct ttttaatact
     3421 catttaaaaa aaatatatat tacataaaat accaattata acaatctttt attagtaaaa
     3481 actacttata tataaatttt actgatagga ggattcttat ggatgatgtt tcaagacaaa
     3541 atgctataaa agctttaaaa caaacaaaaa tggaaatagc aggagagtat ggaatgaatt
     3601 atgaagatgc ttttgaaata atagaaaatg cgtctaataa aggtgtttta gaaggttact
     3661 ttaaaaagct tgaaaagaaa aaaaatttag gtcaaggtat atctaggcat ttagaataaa
     3721 aaaagttaaa tgtgagaaaa taagtttaaa taaactttaa gaggtgacat cgatgaaaaa
     3781 attagctagt actgctttgg ctatattaat tgcgcttact cctttaagct tttcttttgc
     3841 aaataataag gaaaatgctg atgccaacca actaaatata tcttcaaaat cagcaatttt
     3901 gatggatgtt ggtagtggac aaattttata tgaaaaggat gctcataaaa agcttcctcc
     3961 tgctagtgta actaaggtaa tgactatgct acttatagta gaagctttgg attcaggcaa
     4021 gattaagctt gatgatgaag ttcaagtaag tgaaactgca tctagtatgg gtggtagtca
     4081 aatattccta gagccaggag aaactcaaaa ggttgataca ttattaaaag gtatagctgt
     4141 tgcttctgca aatgatgctt gtgttgctat ggctgaacac ttagctggaa gtgttgaagg
     4201 atttgttgat agaatgaatg ctaaagcaaa agagttaaat atgaatgata ctcattttgc
     4261 aaatacaaat ggacttccag ttgcaaacca ctatacatct gctcatgata tagctttaat
     4321 gtctagagaa ctattaaaac acgaaatgat aagtaaatac ttaactacat ggatggataa
     4381 ggttgtagta gggaagaaac aagttacagt aggtcttgca aatactaata aacttataaa
     4441 gcattatcaa ggagcaactg gtgttaaaac tggttttact caagaagcaa aatactgttt
     4501 atcagcatct gcaaagagag gtaatactca tctagttgct gcaactttag gagcagagac
     4561 ttctccagaa agatttaatg atgcctctag ccttttaaac tatggatttg ctaattatga
     4621 aagtgttaag ttatgtagta aaggtgataa tatagcaaca cttacattgg ataaggctga
     4681 tgaaaacaaa gttaaattgg ttgctaaaga agatttaaat gctcttatta aaaaaggaag
     4741 ctctaaagaa tttgagaaga aaattgaaat agtaaaaaat cctaagatgc caattaaaaa
     4801 gggaactgtt ttaggaaaaa taaagatatg taaagataaa aaagtaatag gcgaagtgga
     4861 attgataaat actaaggata taaataaggc aagctatcta caaatgcttc aaagaataat
     4921 tgataatatg atataaataa ttatcagatg aaatataatt ataatacaca aagatatgat
     4981 tataatacac ataaaaaata cccttatgta gttatttgcc ataggggtat ttttatttac
     5041 catatagaag tattttataa tcttatgatt tttatagaaa tatagaaaag tttatgtatc
     5101 ttaaaatatc atttgattta atatcatata tagggttgtt taacaattat ctaagtaaag
     5161 gcatactacc agtaagttta ttcactttct ttttattccc ataaattgct atcccaaaat
     5221 actgtaactc atcttctgga acactagcaa tcttttgtgt aaattcttca taaagattgc
     5281 aactttgtgc tacatctgaa aaatctacaa cagttaaatc gttaaattcc aaagtatata
     5341 attttttacg taaatctttt attatttctt tgtttccttt taaaataggt acaggaatgt
     5401 caataattcc taagtggcta ttacctgttt tgtcaattac atctggccca acagtttctg
     5461 gggcatgttt tcctagtgta attcccatta tagctgctgt attggatata attcccattg
     5521 gtaaattttc gtctattacc atgacacatt ttgaattttt attttccatt tttatttctc
     5581 ctttttacct tatttaaagt tgtttgaatc atcatttata aatatatttt gatactgctt
     5641 tggtgtaaga ccaataaaat tcttaaaaaa gtttgtaaag tgactttggt ctacaaatcc
     5701 tgtttgtatc gcagcatcta taggttctac gcctttttct aaaagctttt tagctttgtt
     5761 tacccgtata ttttctaggt atctatatgg tgtaatacct tttaatttcg taaaattacg
     5821 aagtagagag tacttattca tacctgctat agtgcttaat tcgtcaagta ctatatgttc
     5881 agcataatta ttttctagat aatcacatac tgcttgaatt tctatatttg tattttccaa
     5941 gttagattgc aaatttggct ctgtatgttc ttcaattaat tgctcaatta ggaagaaaaa
     6001 aagctcttcc tttttaaaat ccaattcttc ttccatgatt atatagtgta attcttgtag
     6061 tagaggtact agctcactgc gaaatataac aggttgatta aattttggta aataattttt
     6121 acctgtaatt tcaaagacgg tttttttcat tatttctggc ttaatattga tacatctgta
     6181 atcaagtact ttatcatcta tttgttcaca agtatgactg tcaaaagggt taaaaagtag
     6241 caaatctcca gtacttgtgg tatactcttt atttttacaa gataaatatc tttgaccttt
     6301 ttctataaaa ccaattacat aatgttcatg aaaatggttt gggaattttt gcattatgcc
     6361 tttaaatcta tatgcttcaa tttttaattc cctatcaaag catactgtgc gttcttcttg
     6421 tctcacttag gtaacctcct ttcatgttat acaaagtgta acacatattt ttttatactt
     6481 cttgtataat attgactaag agacaataat gtcaattcat gtcaatataa tattacactt
     6541 acagtaggtg taattattat attttcacaa tgaatatgat ataataataa aagttataaa
//
LOCUS       DAMJVX010000181         6598 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMJVX010000181 DAMJVX010000000
VERSION     DAMJVX010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.187"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(<1..656)
                     /locus_tag="PC407_003152"
     CDS             complement(<1..656)
                     /locus_tag="PC407_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2498562.1"
                     /translation="MKKDTSHIIKRAEHFQVILIGEGLMVGAVAGLIVLLYRILLGQA
                     GTWLSQILKFVEGSTIKIIAWFAVLAVLAWIVSKLVDWEPMISGSGIPQLEGEMTGKI
                     KQVWWRVLPTKFIGGFLSLMGGLALGREGPSIQLGAMAGKGISRALDRGKTEEKFLLT
                     CGASAGLSAAFHAPLAGVMFSLEEVHKNFSVSVLISVMTASLTADYISANFLGMESVF
                     "
     gene            complement(843..1352)
                     /locus_tag="PC407_003153"
     CDS             complement(843..1352)
                     /locus_tag="PC407_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2498563.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLE"
     gene            complement(1427..1840)
                     /locus_tag="PC407_003154"
     CDS             complement(1427..1840)
                     /locus_tag="PC407_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2498564.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNSDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2277..2936
                     /locus_tag="PC407_003155"
     CDS             2277..2936
                     /locus_tag="PC407_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HDF2498565.1"
                     /translation="MDEKILELLQEIQGNISNMQGSISGIEGTVNGMQGNINYMQGNI
                     NYMQGTISDMQGTISDMQGSINGIEGTISDMQGSINGIEGTISDMQSNINDIEGTISD
                     MQGSISSMDGTINSMQDSITDMQGEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQ
                     SDVTEIKSDVKINRNTLDTVIKHTSSLVEDTSDIKNDLNNIEIVTSKNWNDIAKLKSV
                     K"
     gene            complement(3543..3722)
                     /locus_tag="PC407_003156"
     CDS             complement(3543..3722)
                     /locus_tag="PC407_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2498566.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(3947..4480)
                     /locus_tag="PC407_003157"
     CDS             complement(3947..4480)
                     /locus_tag="PC407_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2498567.1"
                     /translation="MQYISYYHSPIGNILLASDNVGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGEEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     HKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            complement(5298..5870)
                     /locus_tag="PC407_003158"
     CDS             complement(5298..5870)
                     /locus_tag="PC407_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2498568.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGSCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(5932..6195)
                     /locus_tag="PC407_003159"
     CDS             complement(5932..6195)
                     /locus_tag="PC407_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2498569.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDLYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(6195..6416)
                     /locus_tag="PC407_003160"
     CDS             complement(6195..6416)
                     /locus_tag="PC407_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2498570.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYSNPKNRVRAKKGGNMY"
BASE COUNT         2311 a         1135 c          812 g         2340 t
ORIGIN      
        1 tggaatacag actccattcc taggaaattg gctgaaatat agtctgctgt caaagatgct
       61 gtcatcacag aaattaaaac agatactgaa aagtttttat ggacttcttc aagtgaaaac
      121 ataacacctg ccagtggtgc atgaaaggct gctgataatc cagcacttgc cccacaggtt
      181 aacaggaatt tttcctctgt cttccctcgg tcaagagctc tagaaatacc tttacctgcc
      241 atagcaccaa gctgaatgga aggaccttcc cttcccaaag caagaccacc cataagtgat
      301 aagaatcctc caataaattt tgtgggcaac actcgccacc atacttgttt tattttccct
      361 gtcatttccc cctcaagctg agggatacca cttccagaaa tcattggctc ccagtccacc
      421 aactttgata caatccacgc aagtactgca agtactgcaa accaagctat tatttttatt
      481 gtagaacctt ctacaaattt taaaatttgg ctaagccaag taccagcctg ccctaaaagt
      541 atcctgtata aaagcacaat caaaccagcc acagccccaa ccataagtcc ttcaccaatc
      601 aaaattacct gaaagtgctc tgcccgtttg ataatatgag atgtatcctt tttcatactg
      661 ttcctccttc atccaataat tctacacaat ctgaacctcc aatgactaaa gtcatgagtg
      721 tgtacagata ttttttaatc aacaaaaata gatttttatt tactaaatat aagaaaataa
      781 ttgttcaatt tctcaataaa tgtaaattta ttcttttagg tatttattct ccacagtcgt
      841 gcttattcca gcaaaatatt tattagttct tgctttctct gtgtatagat tttacgattg
      901 tcctcaaact gtaatgctaa tttttgttta tgactatcat agagttttgc tttgtctgta
      961 aaagtattta gataatctcg aaaattgata taattattcc atgcgacact attccattca
     1021 acaacatgaa tatggtgagt acgagtattt ttctcaaaat ctcccatagc aaaaagtaat
     1081 tgttttggta catcctgacc tcgaaaaacg attccacatt gctctaacct ttcgaggtat
     1141 aatttagctt cctctaaaga agcaacacct acaacaatat caataatagg ctttgcgcta
     1201 atactgggaa tagaagtgct tccaatgtgt tgaatatcaa tggcagtctt atcccatata
     1261 cttttcaata atataatgac atcttttgca cttttatccc atatcatctg atgttccaat
     1321 aatttaacca taccacattt taaacctatc actatatacc acctcaatat aatccacctt
     1381 tatatatata ttacaatttt ttctatcttg ataaacagtg atctagctat ttcacttttt
     1441 cttgacactc gctacattcc ctatcatgaa tagatataat tcctccacat ttagaacaag
     1501 tatatctctc cttctgcttt tgcataaact tctctaaacc gtactgctga acaaatttac
     1561 tattttccat aaggcttgct tgatatctct tgttataact tttttcaaga ttttttatga
     1621 gtttacaagg aaaattgaaa cattcaaaac aataaaacaa ccctttttga cagacacaat
     1681 cctttatttt acacttacgg cagtgttctg gctttcctgt atcgctgttc aaacaaccag
     1741 cacatggctt tttgtgataa caatgcttat agcaaaccat acaattcatt ccacaaggag
     1801 caaacataat cgtatcaata ctctcttttg gcattttcat aacattttac tcctttcaaa
     1861 aatccaattt gtcgaattgt tatatattca tattgtaaca taaaaaatag ctaaaaaatt
     1921 catgcacttt cattgccaat aaattttcaa ggaaatatga aagtttaaaa ctaagaataa
     1981 aaagaaataa aaaggaagct actaatcaaa atattcaaac acaagtaatc aattatgata
     2041 ttaccagcag ttttaaatac aaatataacc acatatgcct caaaaacact tattatagta
     2101 tcttagacac ttagacaata cacaagattc attaataaaa aagttctcaa tatagatata
     2161 tttaaccatg ttttgaatag cagataatta tatgattaaa aattaaatat gacatagcgt
     2221 ttgtggaata taacacctaa attatgttat aataataaaa caggagtgtg atagtgatgg
     2281 atgaaaaaat attagaatta ttacaggaaa tacaaggtaa tattagtaat atgcaaggta
     2341 gtatcagtgg tatcgaaggt actgttaatg gcatgcaggg taatattaac tatatgcagg
     2401 gtaatattaa ctatatgcaa ggtactatta gcgatatgca aggtactatt agcgatatgc
     2461 aaggcagtat caatggtatc gaaggcacta ttagcgatat gcaaggcagt atcaatggta
     2521 tcgaaggcac tattagcgat atgcaaagca atatcaatga tatcgaaggt actattagcg
     2581 atatgcaagg cagtatcagt agtatggatg gcactattaa tagtatgcaa gacagtatta
     2641 ctgatatgca aggtgaaatt aaagaagtca atagtagatt agacaatatg gaacctgata
     2701 ttaaagactt aaaaactgga caagttgaaa ctagtagtag acttgataca attcagagtg
     2761 atgttacaga aattaagtca gatgtaaaaa ttaacagaaa tacattagat acagtgatta
     2821 aacatacctc ttcattagta gaagatacat cagatattaa aaatgattta aacaatatag
     2881 agatagtcac atctaagaat tggaatgata ttgctaaatt aaaatcagtt aaataatata
     2941 ccatagtaaa aaatacctga aaatttcaca tacttcttta aagttatacc aaattttata
     3001 cagaaatact gattatccga caacacactt tacttataaa taatttatta aaaaatactc
     3061 attttatcct aaaattaaca cgactaaatg agtcttgata taataataaa aaataaggga
     3121 gattgaacac taaaaagttg tgtatctccc ttatattcta taatatagtt atataatttt
     3181 tcagcctaaa ataaaagtcc atctttgatt aaaacatatc cccttatcac ttataaaagg
     3241 ataaaatctt gttttcattt ttccatttat atcttcttgc ttaagagtaa taattttttc
     3301 ttttgatact ttaagtgttt ggttaaattt aaccaatcga ctaagttttg ttcaacatct
     3361 atttttcata ttttccaata cttaatctat tttttataaa taattctttt atttctaact
     3421 cacttacatt ttttacataa aacgaagcat ataaatttca accttatctt gtatggttct
     3481 taatacttaa tgagtagctt aagtatcaat cattataaat tttcccctta tatttaacac
     3541 tcttaccatt tccaaaatat acaaaatata ttttgaacat ttgtacaatg atgaaataca
     3601 atacatgaaa atacaatatc ataactgtta tctaaaaata gaagttcttc aacataacct
     3661 ttcacaaaga acatattatc taagtatttt ttgtctgctc cttgttttcc tgtttgtagc
     3721 atataaatta ctgcatctaa acatataact actcacacga aaaaagaaaa taaacttcca
     3781 aaatacaagc tctaaaacta cctctaaagt tatataatgt ttactaggaa taaggttgta
     3841 taacatatag tttaaagaat agttttttaa aattgcaagt tattgctttc aaaattagat
     3901 gtttatatat caaataaatg attaagaatt tctaaattta atggctttat aatgcactcc
     3961 ctttctttgg gataaaataa tgttgcacag atactttttc taaagataac aattttattt
     4021 ttctatcaat tccaccagca taccctgtca aactcccatt tgttcctaca acacgatgac
     4081 atggaacaat aatagagatt ccattatgcc ctactgcacc accgattgct tgtgctgaca
     4141 ttttagatat tcctttttgt tgagcaactt tatgtgcaat ttctccataa gtgattgttt
     4201 taccatatgg tatttgttgt aaaattttcc acactgtagt ctggaaagga gtacctacca
     4261 tatggagtga tggtgtaaaa ttgggctctt ctcccgaaaa ataaatatct agccatttct
     4321 ttgtctgctc gaaaattggc aattcttttt cttgatgttc ttggtcaaga ttataagcat
     4381 aatacttttg attctcaaac caaagaccag ttaacccaac attatctgat gctaaaagaa
     4441 tatttccaat tggactatgg taatagctaa tatattgcat acaattcctc cttatattct
     4501 gaaaacttca ctcaaatcat tagacacttc ttttctatct gccattatgt aaaaacatac
     4561 taatataatt tccttctgac tacactttcg ttataatatc tatcctcata tcatattagt
     4621 tttatattta cctgaatctg ttcatccttt ttttcctttc cctttgatgg atataaaatt
     4681 catacattta agcttcctac tgtattaact tttatagaag tgtatattat aattttatca
     4741 ctaaatagac aaaaaaatat cgatataaca tcaagaaata ttttactgag tttcgcttat
     4801 taaaagcaac tgaatttcta aagggtacag atgagctaat aagtgatatt gatataaaag
     4861 ttgtttttca ttaagtaatc tattttagta aatgttttaa agaaaaaacg ggatattctc
     4921 caaaagtgta cagaaatata aaaaaagaga tatgattttt tatttctttt gattatccaa
     4981 agagtaaata aaaaatattg taaaaaactt actaacaacg gcaatgcttt gaactatggt
     5041 aatactttga acttttgatt ggactttttt tgctaaaata atatatttaa atagcaaagt
     5101 aatctgttta atcaataaaa aataagagat aaaagaaatc aactaaaacc tttacctctt
     5161 attttgatag tttatcagag ctaatattta cactcttttt atttttgata cttcattaaa
     5221 aatgatgctt attctctttt catttttaat acttcattaa aattgatgct tactctctat
     5281 tcacttttaa tacttaatta gaactgatgc ttacactctg taaactcata aacatccatt
     5341 aatgcttcac caaatctttg atagtgaact acttccctct cacccaaaaa tctcaatata
     5401 tctttcaaat cagggtcatc agttaaattt attaattgat agtacgtagc caatgctttt
     5461 tgttcagcag ccatatcctc atgtaaatca gttaccgaat cgccaaagac attaatataa
     5521 cttgtagtaa agtttactcc attagaatct actggttgaa gaccatgtcc aaaattagca
     5581 taattactac caagtccagc agccttaagc tcttccggag tacaactgcc tattaattga
     5641 tataacactg aagaaattat ctcaacatgg gccatttcct ctgtacctat atcagttaaa
     5701 agtgcgcgag attttccagt tggcatagta tatctttgtt gaagatatct agttgcagct
     5761 gccaactcac cattaggtcc tcccaactga gtcatgatat attttgccat tctagggtct
     5821 gcttgtctta tgttaactgg atgttccagt gttttttgat aaatccacat attatcttac
     5881 tcctccttaa tatatattta ataataattc taattttaaa acttaataat tttatttata
     5941 aaatgatttg tcccatggcc aaggttcaga tatccatgac caaggatatt cacttgattc
     6001 atatccaaaa ttagttaatg ctccatattt agattcataa tcacacacag cttgtgtaaa
     6061 tttatcgcag tatctattgt ataaatcaag agctttctta tcccttggat tgttatctaa
     6121 atataaattt aattctatac aggcaaattg atattctgaa attctctcta aaagttctct
     6181 tctatttaat gacattagta catgttaccc ccttttttag ctctaactct atttttagga
     6241 tttgaatata gccaatcatt atagttattt gactctatta aatctaattc aggaaataaa
     6301 gtacctcttt ttaatgctgt ttttggattg tatgtgtcat cataaatttg acgattgatg
     6361 tatggtctag caaattcaca acaatagtct tgtttatcct cttttaaata tcccattaat
     6421 tatcctcctt aatttttaga attttaaaat agcattattt ttaaaaattg tatacttgtt
     6481 ctatattata gtcagtagtt ttaataggtc tttaataaga ttaaataatt aaataagtat
     6541 ctttttttgt tcttatttta cctataaaaa ttttcctaat taatctttca taaatatc
//
LOCUS       DAMJVX010000182         6555 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMJVX010000182 DAMJVX010000000
VERSION     DAMJVX010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.188"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            113..1108
                     /gene="ilvC"
                     /locus_tag="PC407_003161"
     CDS             113..1108
                     /gene="ilvC"
                     /locus_tag="PC407_003161"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDF2498571.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            1161..2849
                     /gene="ilvB"
                     /locus_tag="PC407_003162"
     CDS             1161..2849
                     /gene="ilvB"
                     /locus_tag="PC407_003162"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDF2498572.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKTFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWSAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKAGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSNMEELGKVLDVIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(3207..4337)
                     /locus_tag="PC407_003163"
     CDS             complement(3207..4337)
                     /locus_tag="PC407_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2498573.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVKHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(4416..5123)
                     /locus_tag="PC407_003164"
     CDS             complement(4416..5123)
                     /locus_tag="PC407_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HDF2498574.1"
                     /translation="MSIKNGKDFLYVIWKDPKSRRQFIIGKLAKNGLYEFSYEYEIDE
                     AIKAGFNALISFDDISKTYYSEDLFSPFASRLPDKKRKDINKILDKYDMSEYDTYELL
                     KRSGARLPIDTLEFVDPIPLDIDKNIIRYFYIAGVRHYAGCEGHECDNSIELESNEAL
                     VLELEPENKFDPHAIKVFDKKENHIGYIPRYYSGELTSIINRGLKYNCIIKFVNKENN
                     CDECIKVKLEILFDEYN"
     gene            complement(5136..6005)
                     /locus_tag="PC407_003165"
     CDS             complement(5136..6005)
                     /locus_tag="PC407_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HDF2498575.1"
                     /translation="MIKNFDEWKEYDGFSYGSGRSEKIWLINEKTKDIGLFKFPKTKE
                     TGEYWAEKLACEIASLLKIPCANVEIGTYNKRIGSMSYLINKNDEKLIEGVWLINKYY
                     PCYNTDKLYDYKNNEYYSLKMIFKSIEEYNIKNDFLKMLIFDYLIGNRDRHQNNWALL
                     ESQNKITLSPLYDNGSSLCCYVNIENIDSFFTDHQRFEALINTRSRSRIRIDGKNKKE
                     PTHLEVLKYLNSNYYEQTIDFVEKIINLMNEVNINTLIDNYSENIISPKLKKLLKIYL
                     KEKVLRIKNIYNL"
BASE COUNT         2247 a          835 c         1004 g         2469 t
ORIGIN      
        1 attatataaa tttagaattt aaatctgttg aataatatta aaacaatgat aatgagtatg
       61 aatttctaat aaaaataaat atcataaaaa taagaaaaag tattgggagg aaatgaaaat
      121 ggcaagaatg tattatgaaa aagatgtgga tttagaggtt ttaaaaaata aaaaggttgc
      181 agttttaggt tatggaagtc aaggacatgc acatgcacaa aatcttagag ataacggtgt
      241 acatgtagtg ataggtcttt atgacggaag taaatcagca caaaaagcaa aagaagatgg
      301 atttgaagtt aagagtgtag cagaagctac aaaagaaagt gatttgacaa tgatgttaat
      361 gcctgatgaa aaacaaaaga aagtttatga agagagtgta aaggataact taaaagaagg
      421 tcagacatta gcttttgctc atggattcaa tatacattat aatcaagtac aaccaccaga
      481 atttgttgat gttgtaatgg tagctcctaa aggacctggt cacttagtta gaaatgtatt
      541 tacaaaggga agtggagttc ctgcactatt tgcagtatat caagactaca ctaaaaaagc
      601 tacagagaca gttttagcat atgcaaaagg gataggagca acaagagcag gtgttttaga
      661 aactactttt aaagaagaaa cagaaacaga tttatttgga gaacaatcag tactttgtgg
      721 tggaataagt gaacttataa aattgggata taaaacgctt gtagatgcag gttatcaaaa
      781 agaagtagca tactttgaat gtttacatga aatgaaacta atagtcgacc ttatatatga
      841 aggtggattt gaaagaatga gatatagtat aagtgacaca gcagaatatg gagattatgt
      901 atctggtaaa agggtaataa ctgatgctgc aaaacaaggt atgcaaaatg ttttagagga
      961 tatacaaaat ggaaaatttg caaaagcgtg gattaaagaa aatgaagaag gacgtgaaaa
     1021 cttcctaaaa actagagaag aagaatataa tacagaaatt gccgaagttg gtagaaatct
     1081 aagaagtatg atgtcattct taaaataaat atatattaaa tggattagtt tgagggaaag
     1141 agtggtgtat agtagtgcgt atgaatggag caaaggtaat tttagaatgt ttaaagaaag
     1201 agggaattga cacaatattt ggatatcctg gtggagccgt gatacctctt tatgatgctc
     1261 tatacgatta ttcagatgat tttaaacata taaggacatc ccatgaacaa ggtttagttc
     1321 atgctgcaga tgggtacgca agaagtacta atacagtagg agtttgtttt acaacatctg
     1381 gtccaggagc aacaaatgcc ataactggaa tagcaacagc ttttatggat tcttcaccta
     1441 tggttgtaat ttcagggcag gtgccaacta gtttattagg aaaagattct tttcaagaaa
     1501 ttgacataac tggagcaaca ttatctatga caaaacataa ttatttagtt agaaacacta
     1561 aagaattagt tcctacaata aaagaagctt ttagagttgc aaattcaggt agaaaaggac
     1621 ctgtacttgt agatgttcca aaagatttat ttttagcaga aatggatttt agtggtgagg
     1681 actatgattt atgtcaaata gatgattata tggattataa gagtgatttt gatctagatg
     1741 atgaaactaa tattaagctt ttaaatgaag caatagatat cataaaagaa tctaaaaagc
     1801 ctgtaatata tgcaggtgga ggtgttaaat cttcagacag tgaggaaatt cttgaaaaat
     1861 ttgcaactaa gatagataca cccgtactaa atacacttat ggggcttgga aatatagata
     1921 gaaagaatga actatctctt ggaatggttg gaatgcatgg aagtagagaa agtaatttag
     1981 cactttctaa ttcagattta gtaatagcga taggtgcgag atttagtgat agagtgataa
     2041 gtaaaagttc tgaatttgct aaaaatgcaa aaataataca catagacata gacccatctg
     2101 aaataagtaa aaatatagaa tctaatgtat ctttggttgg agatgtaaag ctagtcctta
     2161 gtttattaat tgaaagggtt gaaagtaaga ataatagcaa ttggaaagaa gagataaaga
     2221 catttagaaa gagtgagggt gtacaaacat atgaatttca tccacaaaat atacttaaaa
     2281 aaataaatga aaaatatgaa acattaaaga aacccactgt ggtagtaact gatgtcggtc
     2341 aacatcaaat gtggtctgct aaatactgga attttaaagg taataaaagt tttataacat
     2401 cagcagggtt aggaactatg ggatttggtt taggtgcagc tattggaact aaagctggta
     2461 atgtagataa aaatgtggtt ttagtaactg gtgatggaag ttttagaatg aactgtaatg
     2521 aactagctac agttgcaaat tataatgtac ctatgcttat tttactactc aataatagaa
     2581 cattaggaat ggtaagacaa tggcagaagt tattttccaa tcaaagatac tctcaaactg
     2641 atattaatga aaatgtggat tatgttaaac tcgtaaatgc atataatatt gatggatata
     2701 aggtatccaa tatggaagaa ttaggaaagg ttctagatgt gatagatttt aataaacctg
     2761 tatttttaca atgtgatata gataaggatt atgatgttta tcctattgta gcacctaatg
     2821 atgcattgga aaatttgata tgtaactaaa acttatactg aatattgata aaatttttta
     2881 ttaaattttt attggtattc agtttttgtt tagagataga ttggcatttt ttatttttgc
     2941 aaataaatca atgaatatat aattaagaac ttattaaatt ttaattatac aatttagaat
     3001 agtattacaa cttaagtaaa tattaagttt tattcgttag atttattatt aataccttat
     3061 attagttatg gttgttatat actctaaagc cttgaaatta caagcgtata gctgactata
     3121 taaatttgat aattttagct tttaaaatag ataaattaga ttttgccacc catttgccac
     3181 cgtattatat ttttgggtgg caaactctaa tttgtaattt tttcaaatat atctacagtt
     3241 tcatttttca ttttatcagt tacatgtgaa taggtatcca ttgtagttga taattggcta
     3301 tgacccaaac ggttttgtat gtctttaatg ttagcaccat tttctaataa tagagtagca
     3361 tgtgcatgtc ttaaagaatg aaaatggaag tcattgttta aagctactcg aatttgtctt
     3421 actatagtgt ctaaagtgtg agtattcact tgttgaccat tttctttggt acatacccaa
     3481 tcactatcaa agtaaaattc tccgtatttt aatttcattt tcttttgata taatttatgt
     3541 tcttttaatg cctttattaa agtgtcacct gtaaatatag ttctgcaaga gctttctgtt
     3601 tttggttgcc ctaattcaaa cattccattt ggttttttaa tcaaagtatg ctttactgtg
     3661 atagttttat tatcaaggtc tatattatcc cattttagtg caataatttc acctcttcgc
     3721 ataccagtat gaaatccaat tagtaaaact atacgttgaa atgaatcttg aggaaatata
     3781 tttagtattt gattaaattc ttctaatgta atagttttaa ctttattagt ttctgtttta
     3841 gatttagttt ttggtatgct tacatattgc atagggtttt ctcgtatgtg tttgtaagga
     3901 tggactgctg attttaatga cctatgtaat atggctttta atacttgtaa tgtattttga
     3961 gaataatcct ctttgtactt tttatttatg aagttttgta gtattgcagg agttaaagct
     4021 tttactttgt aagctcctag ttttggcttt atatgttttt ctatgtttat tcggtagctt
     4081 tcttgagtgt tgtatttaca gttgagtaaa acatattctt tgtaccaaaa gtctaggtag
     4141 tctgataagc tgatgttact ttcttcaaag actatgccag agttttcata ttcatttagt
     4201 gcttctctta gtgctttttc tgcttctttt tttgtattgc caccaactct ttctactttt
     4261 tttctttttc cttctactat gcctaggtca aagtagtaat accatttgtt gcttcttttt
     4321 cttactccac ctttcataat agtatccctc ccttttagaa tgtatgtttg tttggtgttt
     4381 atataaaaaa tgtctcctat atttacaaga agacattaat tatattcgtc aaataatatt
     4441 tctaatttaa cttttataca ttcatcacaa ttattttctt tgttaacaaa tttaataatg
     4501 caattatact ttaatcctct atttattata cttgttaatt ctccactata atatctagga
     4561 atgtaaccta tatgattctc ttttttatca aataccttga ttgcatgagg atcaaattta
     4621 ttttctggtt ctaactctag aactagagct tcatttgatt ctaattctat tgaattatca
     4681 cattcatgtc cttcacaccc agcataatgt cttactcctg caatataaaa atatctaatt
     4741 atatttttat caatgtccaa aggaattgga tctacaaatt ctagtgtatc tataggtaat
     4801 cttgcaccac ttctttttaa aagttcatat gtatcatact cagacatatc atacttatcc
     4861 aaaattttat tgatgtcctt tcttttttta tcaggtaatc tacttgcaaa tgggctaaat
     4921 aaatcttcac tataatatgt tttactaata tcatcaaatg atattaaagc attaaaacct
     4981 gctttaatag cttcatcaat ttcatattca taagaaaatt catacaatcc atttttagct
     5041 aactttccaa ttataaattg tcttctactt tttggatctt tccaaataac atataaaaaa
     5101 tcttttccat ttttaattga cattttatcc ctctttcaca aattatatat attttttatt
     5161 cttaacactt tttcttttag atatattttt agcaattttt ttaactttgg gcttataata
     5221 ttttctgaat agttatctat taatgtattt atgtttactt cattcataag atttattatc
     5281 ttttctacaa aatctatagt ttgttcataa taatttgaat ttaaatattt tagcacttct
     5341 aagtgtgttg gttctttttt attttttcca tctatcctta ttcttgatct tgaacgtgta
     5401 tttataagag cttcaaatct ttgatgatca gtgaagaaag aatctatgtt ctcaatatta
     5461 acataacaac ataatgatga tccgttatca tatagaggac taagagtaat tttattttga
     5521 ctttctagta gagcccaatt attttgatgc ctatctctat ttccaatcaa gtaatcaaaa
     5581 attaacattt ttagaaaatc attttttatg ttatattctt ctatagattt aaaaatcatt
     5641 ttcaatgaat agtattcatt atttttataa tcatataact tatctgtatt ataacaagga
     5701 taatatttgt ttattaacca cacgccttca atcaatttct catcattttt attgattaaa
     5761 taactcattg aaccaattct tttattatat gtacctatct caacattagc acaaggaatc
     5821 tttaacaaac tagctatttc acaggctaat ttttctgccc aatattctcc agtttctttt
     5881 gttttaggga atttaaacaa gcctatatct ttagtttttt cattaattag ccagattttc
     5941 tcgcttcttc cactaccata agaaaaacca tcatactctt tccattcatc aaaattttta
     6001 atcacgaaaa taatcctctc aaacaagtat tttatattct tattgtatca tttgattcta
     6061 aatttgcaag aattttatta tccataataa gcttaactta aaataaaaat tttttctaga
     6121 ttttatttat tccactttta acttcaagct ttattacata tggaatatgt gtttactcat
     6181 caaaattact actgtgtata atggttttac tttataaata cttaatttta gttttctata
     6241 tttatttggt aatttttttg agtgttgtta agtaagaaat attttttgta ccaaaaggta
     6301 agctgatatt gctttattca gatactatgc ctaggtcaaa gtagtaatac catttgttgc
     6361 ttctttttct tactccacct ttcataatag tatctctccc ttttagaatg tatgtatgtt
     6421 tggtgtttat ataaaagagc agattaactg ctctttatat acttttgtta tgtacaatat
     6481 tatcttaatt ttgtttatct ggtttttact taattatttt cttaatattc ttacaatttt
     6541 ttagctttta tgtgt
//
LOCUS       DAMJVX010000183         6542 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMJVX010000183 DAMJVX010000000
VERSION     DAMJVX010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.189"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            81..1823
                     /locus_tag="PC407_003166"
     CDS             81..1823
                     /locus_tag="PC407_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HDF2498576.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEDLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            complement(1987..2181)
                     /locus_tag="PC407_003167"
     CDS             complement(1987..2181)
                     /locus_tag="PC407_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498577.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(2178..2759)
                     /locus_tag="PC407_003168"
     CDS             complement(2178..2759)
                     /locus_tag="PC407_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2498578.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            2927..3745
                     /locus_tag="PC407_003169"
     CDS             2927..3745
                     /locus_tag="PC407_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2498579.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNNTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDSVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            4470..5291
                     /locus_tag="PC407_003170"
     CDS             4470..5291
                     /locus_tag="PC407_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HDF2498580.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDITVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLEDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(5601..6380)
                     /locus_tag="PC407_003171"
     CDS             complement(5601..6380)
                     /locus_tag="PC407_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2498581.1"
                     /translation="MNYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKVFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVVLGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT         2456 a          885 c          834 g         2367 t
ORIGIN      
        1 gttaaatttt cttaacatgc attttttata tttaatatag taaaataatt aataactata
       61 tccatgattg gaggattttt atggttaaat ccaagaagaa aatatttgtt tttctaattt
      121 taattgcagt ttttattata tttgcatcat acataagaaa ttatactaat gatacaaaaa
      181 aagattctgt tagaggaact attccttttg aaaaaatgga atataagcgt cctaatatac
      241 agtcaatttg cgaaaacatt aacaagtgta acgacaaact tttaacagct aaaacagcta
      301 aggaacaatt aaatttattt aatgaagttg ataaaattta tcaggatttt tacagtacac
      361 ttactatagc aaaaattaga aataacatag attcatcaga cgaattttac tctaaggaat
      421 ataagtatct tatgagtaaa tcagtagatg ttgacatgtc atacaaagac tttcaagata
      481 gatttgtaac ttctaaattc tctaaagaat tagaaaaaga acttggaaag gatacattta
      541 attatctaaa gaacattgga aaacttaatt ctaaagaagt tgaagattta cttaaaaaag
      601 agcaagacct agttgtaaaa tatgaagact tattagctaa atctacagta tcaatagatg
      661 gtatagaggt agactttgaa gaagccttgt caagaccaaa tcttagccct gaagaatatg
      721 tgaaaattta ttcagattac ttaaaaaaat acaatcctat atttggtaac atattcttag
      781 aacttataca gacaagaact gaaatagcaa gtaagcaagg atttaagaat tatatagatt
      841 atgcctatat gaacctaaac aaagactatt ctcaagaaga agctaaaaaa tttagacaag
      901 atgtaaaaga ttacatagtt ccattatata gagagatttc atctaaacca tctgattctt
      961 ctatttatat taaagtatat aaaaatcgct catttaggaa gtttgataaa gtacttgaag
     1021 atatatctcc aaaactaaaa gagagcttcg actatatgaa aaaatatgac ttgtacgatt
     1081 attcttctgg aaaaaacaaa tcgcctggag gatatactac ttatataaat aaatataaag
     1141 cacctttctt gtttaataca tgggacaatt cttttttagg tgttactagt tttgctcatg
     1201 agtttggtca tttttataat tactacaaca gtattaactt aaataataag atgcaaccaa
     1261 gtatagatgt atgtgaagtt cattcaacat ctttagaaat tctattttat aaatattttg
     1321 atgatttttt tggtaaacaa tctgaagcta taaaaaaaga acacttatcg attgttttaa
     1381 atactattat tgatgcttgt ctttatgatg aatttcaaga aattatatac aagaatccat
     1441 atatgagtct aaatgatatc aataaattgt tttttgattt agaggaagaa tatggagttt
     1501 ccaataatat acttagagaa aaaaatgctc ctttttggat acttgtatct cataacttcc
     1561 aagttccttt ttattacttg agttatggtc ttgcatctga tgtatcactt cagatatggc
     1621 aattatcaca agaagattac agaaaagctg ttgatgtata tatggatttt ttaaatcaaa
     1681 atactgatgc tggatttaaa gatgtggtag aaaaggtaaa tttacaatta cctttccaag
     1741 atggaaattt agaggaaata agctctactt tatatgatta ttttggtata gataatccac
     1801 ttgaactaaa aaatgcttct taatttctaa tactttatac tcactcttaa gatttagtat
     1861 tagattgaaa taagtgatat tttttcacac acaattaatg tttaataaaa ttatgtggta
     1921 aagtatcaga aatttttaag actcttgtct ttataatttc tgatacttta tttattaata
     1981 atattattat ttttttatat atataattaa aaggattgtg gaaatcaata aaaatatccc
     2041 tgtaaaaata gcactattca tatggaactt aatagaactt gcatctgtta ccttattatt
     2101 cttaatccag aacccacaaa ccatagttga aagaaaaagt aaaacagtga taagacttga
     2161 accaatagtt ataattttca tcttacatca acatcctctc caataatatt ttctattaaa
     2221 atatcaatga atttatctcg ctcatattta ttctttatat ttataccact gtataactga
     2281 aatgattctg tagataaagc agaaatctgt attggtaaaa taatttgcag ggcaatgatt
     2341 cttagactca tttcatcttt tttatttcca aaaattttac gaagcatagg gacaatatca
     2401 agttctgctt gcatatctcc attggttata cattgattca acacaaaagg aagcactttt
     2461 tcataatgta atccagtatc acacaatttt tttaataaat ttttcaaatc ttcaataggt
     2521 cttagagtat atacactatc gtcatatagc tctgctatta tatttgacat cacatctcct
     2581 atggcagtac tcaacaaatt atcctttgtt ttaaaatgat aatttattaa accaactcca
     2641 acatttgcac gctcagcaat ttttctaact gtaatctcct caatatcatc tacttcattt
     2701 aaaagattca atgtaacttc aattatacgt tctctagcat ctttatcatt acttttcata
     2761 atagacctcc aaaataatta acattttagg ttctgaacat gttcagtata atattttttt
     2821 catcatatgt caatataatt taattataat tttagttgac tctaacatta tgatatggtt
     2881 tatattaata ttaagctaaa agtaccttta taaaggagac aaaaacatgt ttaagatagg
     2941 cgatttttca aagctaagta aaattagtat tcgtatgtta cgtcattatg atgaaatagg
     3001 acttttaact ccttctcata cgaataacac taatggatat agatactata gtgcagacca
     3061 actttctact acaaatagaa tccatgcact aaaagatatg ggctttggct tgtattctat
     3121 aaaagaaatt ttgacagaat ataacgataa agaaagtctt ataaaatatc taaatatcca
     3181 ccattctcaa gtaaaagaac aattagaaga tacacagaaa aaactattaa aaatagaaac
     3241 cactattaaa agaattggag gaaatgatat tatgaaaaat tatgatgtaa ctataaagaa
     3301 ttttgcacca aaatatatga tgacacttag aagggttata ccaacttatc aagacgaagg
     3361 aatgttatgg catcaagcat ttttagagac aaaagaccag aatgttcaaa ttgaaccccc
     3421 aaagtattct aaagcagtct tttatgatac tggatataaa gaagattctg tagatgttga
     3481 agtacaagtt gctgtatctg gaaagtataa agatactgaa catgttaaat ttaagactgt
     3541 tccaagtgtg actgctgcaa ctgcaattgt taatggaaat ttcaatcaag ttgcagacgc
     3601 ttgtgaggct attggtaatt ggatatcaga taataactat gatgtggatg gtcctatgtt
     3661 taatatttat catgtaagtc ctggcaacga tagtaatcct gataattggg taacagaggt
     3721 atgcttccca gttaaaaaga agtaattttt atattaattg gataatattt taatttgtta
     3781 aacaaattac ttttagtatt atgtataaat acctacaatt taattaggat attatcttaa
     3841 tttgttaaac aaattacttt tagtgttatg tataaatatc tacaatttaa ttaggatatt
     3901 atcttaattt gtcagataaa tcacttgtga tgttatgttc gaatgactag aatttgatta
     3961 ggatattatc ttaatttgtc agataaatta cttataagat tatatacaac ccttatactc
     4021 tagttaaata tttgaatcac taatataaat tagtttttaa caaaacaaag agtaaagtac
     4081 aaataattcc tttgtactct actcttttta actttctttg tataataata caatgataaa
     4141 tataaaaaat tatatattcc attctataga aatatgttca ccaataacat atatgttatt
     4201 aatcaatatt ttatatcaaa attacatgaa ccttatagaa taatcatatc ttattacaag
     4261 aaaaatgtct tatctatatt gaataaagtc attcggctca tatacaaagt caatttcatt
     4321 gagaaatatt taatataatc ttataaaaat ttcatactat tctttcatat ttacatgtga
     4381 tataatttat taactcataa ttttgttaat cagttgtgat tgtagattat ttatataatc
     4441 taatattttt agaaggtggt gtaataactt tgaaattaaa aattgttggt agtggcggta
     4501 tgtcaattat tccaagttca ttttgtaaat gcaaaatatg tgaagaagcc aggcaaaaag
     4561 gcggaagata tgaacgatta ggaccaagtt tatatattga tgatataaaa atgctgatag
     4621 acacacctga agatattaca gtagcttgta acagacaaaa aatatcggaa gtaaaacata
     4681 tttcaatttc tcaccatgac ccagaccatg ttaagggtat tagaattgtc gaaaaaatag
     4741 gatgtgactt tatcacagga gaaaataatc caattggctt ttatgctctt cctgaagtaa
     4801 ttgaagatat aaaccgaacg aatctcgatt gtcttaaata ttatggtgat gttataaatt
     4861 gcatttctat taatgaaact tcgcatgtaa agataaataa ttttgatatt gatttgatta
     4921 acaataaccc agacaggaat ataaccttct atgttatcaa agagaatgaa aaaaaagtca
     4981 tatatgcctg ctgtaatccc aagccattta ctcatagtga tatgtacttt gatgctgatg
     5041 ttttaataat aagtctagtt tctgatgatg gtatattaaa tgatggtaca aaacttgaag
     5101 acacaccatt taaagatgaa atatttactt tagatgagat tgttgaaatt aaaaactatt
     5161 atagaattaa aaagattatc attacacata ttgacgaaat gtggggaaaa tcatatggtt
     5221 attacagtga attagaaaag aaacttgata acatttattt tgcatatgat ggtatggaaa
     5281 ttatagtcta aatcattaag taataagcat aatgcaaaat aagaaaactt caatttaaca
     5341 gtatagttaa actatgtaat atcaacatat ctcagttttt acaaaatatc atagtattta
     5401 tctttataaa atttcaagtc ctacatattt taatacttca atatctaaat aaagtaaaat
     5461 caaaaatata ttttttatac attatagacg gcttatcggc actagataag ccgttttgtc
     5521 acaattatta tttgctcaac aaaacttctt ataatatata atagattgaa tctccaactg
     5581 atacttaatt accgaaactc ttattcctta atatgttgtt gcattaacca aaccttcatc
     5641 atcatttttt gtggcaatag ttttgctaat aaatgactgg attttgtatt tataccatac
     5701 acagaaatat ccttattttt ctttgcatcc ttcaatgctt ttttggcaac aacgtctgga
     5761 gacactatgc ctgggaaatt ttttgtccct ttttcagcta caactatacc tctcttaaat
     5821 agatttgtac tcatccaacc tggacataca gcaattgcat ttatcccttt actcttaagt
     5881 tcaacattta atgctcttgt ataatttctt ataaacgctt tagttgaact ataaatattt
     5941 tgatatggca atggctggaa tgatgcttgt gaagaaatat tcataatatg gcttcccttt
     6001 tgcatatatg gaatacatat aagtcccaat actacaacag cactaatatt taaatcaatc
     6061 atattaatag attcatctat acttaaatca ttgtatgaac agaattttgc aaaaccagca
     6121 ttgtttataa ggtattttat acatacattt tctttactta aaaatgtacc aatttctttt
     6181 acttcttcta tttttgataa atcttttgaa aaaactttta ccttacttcc aaattcatct
     6241 actaattgat ttaatctcgc cttgtttctt gcaactgccc atatttctgt tatttcttct
     6301 tcatttatta ataactttag aaactcttta ccaaaaccac tattagcacc cgttataatt
     6361 gcaatcttct tataattcat aattattctc ctttaccata agtttatacg ctcaaactgt
     6421 aaacttattt tttacaaata aaacaccctc tattcagatg ttatattcat atcatttgag
     6481 atattatatt tatatcattt tcaataattg ttatatagca taaaaaatag tgttaatatt
     6541 at
//
LOCUS       DAMJVX010000184         6537 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMJVX010000184 DAMJVX010000000
VERSION     DAMJVX010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.190"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            119..427
                     /locus_tag="PC407_003172"
     CDS             119..427
                     /locus_tag="PC407_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498582.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(589..2379)
                     /locus_tag="PC407_003173"
     CDS             complement(589..2379)
                     /locus_tag="PC407_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498583.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(2379..4106)
                     /locus_tag="PC407_003174"
     CDS             complement(2379..4106)
                     /locus_tag="PC407_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498584.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSARVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(4351..4915)
                     /locus_tag="PC407_003175"
                     /pseudo
     CDS             complement(4351..4915)
                     /locus_tag="PC407_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
     gene            5204..6316
                     /locus_tag="PC407_003176"
     CDS             5204..6316
                     /locus_tag="PC407_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2498585.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     AEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
BASE COUNT         2241 a         1038 c          841 g         2417 t
ORIGIN      
        1 ttatctttat aaattatagt tttagattta taatttataa aatatttatg ttctttgtaa
       61 aattttttag aagcttctaa tgttatgcta taaaactatc ttgagaaggg ctgattgaat
      121 gtttaataaa atatatgaaa agaaagaatt tatagttttt caagttaaaa aaggttatgt
      181 agtttataat actagaaaga gttttgaaga aggtcataca catttaaagc attttgaagc
      241 agcaaagaca gctattgatt tagcaataaa taaaaagatt cctagaagca aagatggata
      301 ttatctgact agtttaattc gcataagtga tgatggttat tacattgata agttaagcga
      361 attgttatat gttagagaac aaaaaggtaa aaaagaaaag tactgtaatt ctggttatca
      421 gatgtaacaa gactagttgt gaattataag taccaatcat aaataccttt agtcttaaaa
      481 tcaaagagta tcagtaaatc tataaaaaaa caacttactg atactctttt taattatatg
      541 attaatattt gaatttgata caaaatttta tacaaaagtt tatgttatct attctgattc
      601 tttgtctgca aattggctat tataaagctt ctcataaaat ccaccctttt ccattaattc
      661 atcatgattc ccctgctcta taatacttcc attattcata actaaaatca agtcagcatt
      721 gcgaattgta gaaagtctat gtgctataac aaaactagtt ctaccattca taagatttct
      781 cattgctttt tgaagcataa gttccagcct agtatcaaca gaactagtag cttcatcaag
      841 tatcaatatt gaagggtctg atataaaagc tctagctatg gttaaaagct gtttttcccc
      901 tagagatata ttagatgctt cttcatttaa gaacatattg tatccatctg gaagagtggt
      961 tataaagtga tgaacatttg ctacttttgc tgcttgtata atttcttctt tagtagctcc
     1021 aaatctacca tattctatat tttcaaaaat ggttccatta aataaccatg tatcttgaag
     1081 caccatacca aacattgaac gcaaatcttt acgtttcata tctcttatat ctactccatc
     1141 aattttaata gcacctttct ttacatcata aaacctcatc aataagttta ttaatgttgt
     1201 tttacctgct ccagtaggtc caacaatagc taccatctga ccacttttta cttctgcatt
     1261 taaatcttct attaaagttg aattttctcc atatccaaaa ttaacatgct caaatgtaac
     1321 atttcctttt acattttcta tcttaactgg attttcaaca tcaggtattt cctcttcttc
     1381 gtctaaaatt tcaaatacac gttctatagc tgcaaaagaa gattgtatta ctgtagataa
     1441 ctgagtcatt tgtgaaagtg gctgatttat ctgccatata taacgcacaa atgcttgtaa
     1501 atttcctact gttatccctc cacttactgc atatatagaa ccgacaactg ccacagctgc
     1561 aattccaaag taagttacta atgaaaccat aggattcatc atacttgata taaattgtgc
     1621 cttaaatcca ttttcacaaa gttcttgatt tactttttta aattctttta tagaatcttc
     1681 ttctttacca tatagtttta tttcattaaa tccagtatac atttcttgta cttttccatt
     1741 taattttcca agagcatttt gttgcaatga aaatctactt tgagattttt ttactacaaa
     1801 tttagataca aaataactaa ttggaattat aaaaagagct ataagagcca tatatatatt
     1861 aattgtaaac atcattgaaa gtgccaaaat tatgactaag attgagttta caatttgcat
     1921 aaaactctgt tgcaatgcat ttgatattgt atctacatca tttgatatac gacttaatac
     1981 atctccaaaa ctattactat caaaataact aataggcaat cttcttattt tattttcgac
     2041 ttcatttctt aaatctctca ttgtgttttg aatagcattc gtaagtagaa aacttgaagt
     2101 atacgtaaaa attgcattag caaaatatat acatgctaaa atcaccaata tttttgttac
     2161 ataatcaaaa tttacggaag cccctggtac tccttttgcg atactaatta catctttagt
     2221 caattgtgtt attatcaatc cttcagtttt aggtgcaagt gcattcatag tagctgctga
     2281 aattatacat ataatagcta taataaagga aaatttatat ttctttacaa atggaagaag
     2341 tctaccaatt gttttcttaa aacctttaga ctttttcatt attctaattc ctcctttttt
     2401 aactgagatg ttgcaatttc atagtatatt tcacagtttt ttaataattc tttatgagtt
     2461 cccattccga caacttgtcc ttcattgagt actataatct tatcagcatc aataatagaa
     2521 ctaattctct gggcaacaat caatacaatt gcatcttttg tttccttctt caatttagca
     2581 cgaagtattg catccgtttt aaagtcaagt gcagaaaaac tatcatcaaa aatgtatatt
     2641 tcaggtcttc ttataatagc ccttgcaata gaaagtcttt gtttttgacc tcctgataca
     2701 tttgctccac cttcactaat caattcatca aactttctag gtttttttga tataaactca
     2761 tatgcttgtg caactcttgc ggaatattct aattctgact ctcctgcctt atgtttccca
     2821 aatctaatat tatttgctat gctaccagta aaaagcagtg atttttgagg tatgaatcct
     2881 atcttttttc ttaaagcttt taaatcatat tctcttatat ctactccatt tatcttaatg
     2941 ctaccctctg ttacatcgta aaatctaggt atcaagttaa ttagagtact tttcccacta
     3001 ccagtacttc ctataaaagc gactgtttca cctgtttttg cagtaaatga aatatcctta
     3061 agtacagaag cttctccatc tggataggta aaagttacat tatcaaattc tatgataccc
     3121 ttattttcag tatccttaat tccattttca ggatttttta tcaatggttc ttcatttaaa
     3181 agttctttta ttctatttgc agatacctct gctcttggat acataataaa taccattgaa
     3241 aaaagcataa ttgaaaacat ggcatggaaa agatattcca aaaatgcaac caactgtcca
     3301 acttgaagct ctccaatatt tatcttctca cttgaaatcc aaaatacagc caaaacagct
     3361 atatttaata gtaaaaagaa tgctggttga gtaattgaca taagcttaaa tagtttctta
     3421 gaaacattag cataatatgt atttgttttt tcaaatcgct ctgtttcata atcatcattt
     3481 ccaaaagctc taattactct tattccagtt aaattttctc tcgatataag gtttaattta
     3541 tctagtcctt tttgttgttt tgaagatatt gcctgagata cttttgctat tattgctact
     3601 ccaataatta taaaaggtac tgatatagcc aatactatag aaagttccac acttgttcta
     3661 attgtcatta ttatacttat gatgaacata actggagtta ataaggcagt ccttagtagt
     3721 atattaacaa attgttgaat ttgaaatgca tcattcgttg ttcttgtaat cattgaagaa
     3781 attccaaatt tattgtattc agtatgtgaa aattcttgtg atttctcaaa tatgtcattt
     3841 cttatatctc tggttacact tgttgatacc ttagcagaac aataacccaa caatatacta
     3901 cctacaaccc ctataataga aataataaca atcactattc ccatttgttt tatgtaagct
     3961 atattctgat ttgcaatacc attgtcaatt attttagcta ttatagttgg aatccccaac
     4021 tcgactaatg caaaactaaa tatagccagt atgtttaata ctattaataa cttataattc
     4081 tttaaataac ttaaaattaa tttcatattt ttctcctttc ttaatttttg agatagtgta
     4141 atcatgtaat aatactaaat ttataaatgc ttaattttat aaaaaaagga gaaaagagtt
     4201 aacgcactct cttctccagc atattctttt taatacaccc tccgacaagt cgcttatatg
     4261 ttaacatatt tgacaagtaa tgtcaattca tatagctaaa agtcttttaa atgcttcatc
     4321 tcataacatt gtaacaaatt ggcatgtaat ttatttttgt tttttactgt attccaattt
     4381 tacaagttct ccaatattac taactttctt aaaattcaaa ttagtcttat ctataccaga
     4441 cttaaatagt gaaatccctc tccccaatat tattggcaat atatatatat aataatcatc
     4501 aattaagtta tctttcataa ataattttac tatatcgcct ccaccaacta tccaaatatc
     4561 ttttttatcc ttcttttttc caatttgctc aattaatttt ttaggttcta aattagtata
     4621 ctttacattc tccagttgtc ccattaattg tatttgagta tatatagcat tccttaccct
     4681 tatagtacca ttcatttggg cttaattcat ttgcaatctg tacatatgtg gaatttccca
     4741 taatgatagt atcaattgta tcgtaaaaag tattataatc atcttcctgc tcattcttaa
     4801 attctgaaga tatctcgtct aaaaaactta cactgccatt ctcttcagct atataaccat
     4861 ctaaacttgt ggcaatataa agtactactt ttctcatgtt tactacctcc aacatttata
     4921 gtgtgattat tctatgtaaa catcactttt ttactcattc aataataccc tattaataaa
     4981 tcctcaaact aatttaaata aactttgcat aaaactaaaa atatattata taaataattt
     5041 aaaatcacac taacttattg aaaattctaa ttattacaaa taaatattgc tctctgttaa
     5101 cgctagtttt ataatataat tataagctat taatattata aagccgatat tactaatata
     5161 ataattatag ttaatttact tttgttgaaa ggtggtaaat aacatgaaaa tttcagaagt
     5221 cgctactcca aattttttgc tagatttaga ccagttagaa aagaatatag ataaaattca
     5281 acaaatttgt acaaataata ataaacaact ttggcctatg cttaagacac ataaaagtac
     5341 ttacatagcg agacttcaaa aaaatgcagg tgctagtggt tttctagttg gaacacttga
     5401 tgaaggtgaa gctctgataa aagcaggttt tgaagatatt atgttggctt atccttacat
     5461 gggagaagta aatataaata gaattgtgac tatggcatca aaaataaatt taatctgtgc
     5521 tacagataat atagaatgtg ctaaggctta tagtgaagct ttttctaaat caaaaataga
     5581 gtgcaagcta cttattattg tagattgtgg cttacaccgt tttggggttg aaccagaaaa
     5641 agtggttgaa cttgctactg aaatttctaa gctagaaaac atcaagattg ttggtataag
     5701 ttcgcatcca ggtcaagtgt acggttgtag ttctccagaa ggtgttccag atgtttgtgt
     5761 tcaagaagat tcttctatgg agaaagcata caaagctcta actgataatg gatttaatat
     5821 agaaattgtc gcttctggta gtactccaac agttgaagct gaagctgctt ctaaaactat
     5881 aactgctgta agacctggaa actatgtata ctatgatgct atacagatag gtttaggttg
     5941 tgctactgaa gatatgtgtg ctcttactgt tgtgtgtact gtaatctcaa aaaatagcag
     6001 tggatattat ttaattgatt gtggaagtaa gtgccttgga cttgataaag gtgcacatgg
     6061 aaatagtagt attgttggtt atggtagggt atgtggacat ccagaactta ctattgattc
     6121 actttcagaa gaagttggaa aaattaaagt aaatggtgaa actgatataa aaattggtga
     6181 tagaataaga attataccta atcattcttg ttccactgca aacttgacga gtaatttatt
     6241 aggttttaga ggagatacaa tagaaaaagt catagaagta gatattcgtg aaaattcaaa
     6301 acaaatcatc ctttaaaaat atatttattt tgatattaac atcatgaaag tatatttttc
     6361 ttaaaattat tgttaataac taatgttaat gttggataaa taaaaaacat aaattctaga
     6421 aatataaatt taaaaaaaga ggctgtctca aaattggata tgtccctgtc aagtagacag
     6481 acttaaaaaa ggctatcttt atgcggaatg agttcgatat tcaatcggac tcattcc
//
LOCUS       DAMJVX010000185         6527 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMJVX010000185 DAMJVX010000000
VERSION     DAMJVX010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.191"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            357..1631
                     /locus_tag="PC407_003177"
     CDS             357..1631
                     /locus_tag="PC407_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2498586.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSAIYVKNATEGINF
                     LAKFFKDENPDKFIITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1838..2155)
                     /gene="trxA"
                     /locus_tag="PC407_003178"
     CDS             complement(1838..2155)
                     /gene="trxA"
                     /locus_tag="PC407_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2498587.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2422..3174
                     /locus_tag="PC407_003179"
     CDS             2422..3174
                     /locus_tag="PC407_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDF2498588.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKN
                     KSVEYVLNIKGDKNILKKALEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHTHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3161..4042
                     /locus_tag="PC407_003180"
     CDS             3161..4042
                     /locus_tag="PC407_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF2498589.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNVADFKVQFFTPGLEVDLCGHATIGTFHALLEEGRLNTNKSIFYQETK
                     AGVLSVELKEINNENIFMMEQNTPEFENLDNYRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(4268..5623)
                     /locus_tag="PC407_003181"
     CDS             complement(4268..5623)
                     /locus_tag="PC407_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2498590.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKVEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2512 a          940 c          822 g         2253 t
ORIGIN      
        1 tctaatcaat ttctttacta tttttacaac tttattagtc atattcataa ttatttctaa
       61 ttagtgttta acattttatg taaaatttat aaaaaaatgt atataaatta aaataagtga
      121 ctatactaaa aatatgatac ggataaacaa taagttcaac ctatctcccc caaaatcaag
      181 tgagctacag tttcccccaa ctgtagctca ttttcgtgta atttaataaa catctcaaca
      241 agaattaatt aacaaaaccc cttataaaaa cataacatat attaaacaag ctcttgatta
      301 aaacttgagc ttttttaata aaaaacagaa tagtttatta gataaggggt ttttttatgc
      361 aattcatgga tgtatctaaa tacattgaag gtgcaaacct taaagttaaa actaaaaatg
      421 gtgaaactgt taaaagaata tattttaaca actctgccac tccattagtt ttaaaaaacg
      481 tagtggataa cttaaactgt gagattcctt ggcttactta tattaatgct ccaggaataa
      541 tctcagaaaa aaatacttta aaatatgaaa atgtacgctc tactatcctc aaacttattg
      601 atggagacga agaaaaggat tctgctattt atgtcaaaaa tgctacagaa ggaataaact
      661 tcttagcaaa attttttaaa gatgaaaatc cagataaatt tattattacc acagcaatgg
      721 agcatatggc taattaccta ccttttaaag ttaattttcc tacaaaagtt gttggaatta
      781 cagaaaaagg agaacttgat ttatgtcaat tagagaatat acttaaaaat tatgctggta
      841 atgtatctct cgttacagtt acaggagcta gtaatgtcac aggtataacc actcctatat
      901 atgaaatagc aagaatagct cataagtatg gtgcaaagat attggtggat attgtgcaag
      961 taattcagca taaacctttt tcaatgatgc cacatgaatg tgatgagcat atagattttg
     1021 ttgtttttag tgctcataaa tgttattctc cactaaatgg aggagctctg gtaggaccta
     1081 aaaaattctt tgaaaaattt aatcctgtac tatatggctc aggttctacc aaatttgtaa
     1141 atgataacaa aatactatat gcaaacgtac cccaacgttt tgaagcagga tacccagagt
     1201 attttggaac tcttgcaatg ggaaaagcac tgaacacttt aaataaaatt ggtctaaaca
     1261 gaatttcaaa atacgaaaga gaattatttt tatatgctaa agaagaaatg agtaaaatac
     1321 ctaatataat aatatatgga gataaatcaa ataatgtaat tatcccaaat atatctttta
     1381 atataagtaa tatgtattac aaagatgtag caaaatattt agttgataac tttggaatag
     1441 aaactggagc tggagtagta ggtgcagata tttatgtaca aaaattatta ggtataagtc
     1501 caaaagaagc atacgtcaga tttatgaggg aaaatcctgt aggtcttgtt aggataagct
     1561 taggaatgta caatactttt gatgaggtgg atagctttat acatgctatt aaaactttgg
     1621 cttttaaata agctttttct attctttttt atcaataaat agagagtagt tttgttttca
     1681 caaaaaaaca aattatatca acagtcaatt tcattagcaa ttagacactc atttgtaact
     1741 atacaaacta ttttacacta tacaaataaa aaactcatta caatgagttt ttttgtttat
     1801 atatgatatt tattttaatt acttcaattt actttaatta catatatgat tcaattttgt
     1861 tttttaaatt ctccttaggc ataaaaccta ttaatctatc tacaggtttt ccatccttaa
     1921 atattagcat agttggtact gtagatactt caaatttctg agctaattct atactttggt
     1981 ctatatccac cttcaagaag tttgcttttt caaccatctc atttcctaaa gcttcatata
     2041 ttggtgatag catcttacaa ggtccacacc aagtcgcaaa gaaatctaca actactaaac
     2101 catctttatt ttctacttca tttataaatt cattattatt tattattttt accataatat
     2161 acatctcctt tattatcttc ttgatactaa tatactacgc cctatataac agttcaatta
     2221 ggcacttttt tgtaaccgcc tatttttaat agatttaata ttatgataaa tagatttata
     2281 agattaaata tttataagac taaatatttc tgatatattt tatttttttc ttaatatagt
     2341 tgacattttc tcccttaaat tataaaatca aattaataac tacataggta gttactaatc
     2401 ttttatcaaa ggagtttcta tatggataat ataattaatg aattacaaaa tataggattt
     2461 actaaatacg aatctcaaat atacatatca ttattaaagg aaagcccttt aacaggatat
     2521 gaaataagta aattatcagg tgtaccacaa tcaaaagtct atgaaaatat aacaaagcta
     2581 ttaaacaata atataattat caatgttgga actgacccta ttaagtacat acccataaac
     2641 cctaatgaat tattaaaaaa taagaaaaaa gaatttgata attctatgga taatctaaag
     2701 aatagcttac agatattaaa taaaaataaa agtgtagaat acgttttaaa cataaaagga
     2761 gataaaaata tattaaaaaa agcattagaa atgataaaaa atgcaaaaca agaaattata
     2821 atttcttgcc cttatgaaga aattcttgaa ttaaaaaaag aactcctaat tgcttacaaa
     2881 aaaaatatta atattgaaat actactttta gaaaacaaaa gtctgaaagg tcttgataat
     2941 attcatactc atggaggtaa taaaactaca cttgaaaata aaatgcttta caaaggaatt
     3001 attttgattg tagataatga tgaaatattg acaggaaact tatcagaagg cagtgaggcc
     3061 atttcaattt ggacaaaaaa ctctaacatt ttatatattg gagttcaata cataaaacat
     3121 gaaatttata tttcagaaca aataaaagga ggttataaaa atgaacaaaa ttaagattaa
     3181 acaagttgat gcttttacaa ctataccttt tggtggaaat cctgctggtg tcgttactga
     3241 tgcctctaac atttctgatg aaattaagca gaatgtggct aaagaaatga atttatcaga
     3301 aactgcattt gtttcagagt caaatgttgc agattttaag gtacaatttt tcacaccagg
     3361 tttagaagtt gatttatgtg gtcatgcaac aataggtact ttccatgcac ttttggaaga
     3421 aggaaggctt aatactaata agagtatatt ttatcaagaa acaaaagctg gtgtattatc
     3481 tgtagagctt aaggaaataa ataatgaaaa tatatttatg atggaacaga atacacctga
     3541 atttgaaaat ttagataatt acagaaaaga acttgcacaa atgataggtg tgcatgaaga
     3601 tagacttctt gaatatccta ttataaaggt aaatactgga ctttggtggc ttgtatttgg
     3661 tctaaaatct ttagatgatt taaaaaatat ctctccagat ttaaataaaa tcaaaaaatt
     3721 tagtggtgat aataatataa ttggtataac tccattttgt atagaaacta tagataaaaa
     3781 gtgtgactac catttaagat ctatagcacc atatgttgga gttacagaag accctgtatg
     3841 tggaactggg aacgcttgcg tatcatctta tatagtacat cacaatattt tagataaaac
     3901 tagttttatt ggtgagcaag gtaactttat aaacagacct ggaaaggtat atgtaaatat
     3961 aactagagaa aacaatgaca taacttctgt aaaaattgga ggaagtgctt atactgtttt
     4021 aagtggtgaa attatgtatt aatcccatac ctaataaaaa caaataaaaa tactagctaa
     4081 ctaaagctag tatttttatt tgttattttt atttaataat cacactctaa caaatattat
     4141 aattatatat atcataatat tgtatttaga agtaaaattt tatttttatt attaattagt
     4201 tataaatcaa tctaatacaa cataattaca gtagattaat ctataaaaca taataattac
     4261 ttactgatta tttatcatcc tcaacaagtc tacttaatgt cttatcagat aagaataata
     4321 atatcgctgc aacaaatagt attactggaa ttatagtata tactttaatc atatcaaatt
     4381 taagagtaaa tgcatatata tatccatatc ctttaccagc tataaaagta gcaaatgtcc
     4441 atactcccat aagaacacct agaagcttct taggtgcata cttacttaca aaagagttac
     4501 caagtggtga gaataacatt tcaccaagac ttaaaagaat accaaacatg attatccata
     4561 gtatactagc tttagcagtt tcaggagctc caactcctct tgaaaattct gcaccaacaa
     4621 gcattaagaa tgcaagacct aagaatatta atccaagacc tgtcttttta aataaactca
     4681 tatctccttg tggtctacta gcaagtttaa accataatgc tccaagaact ggtcctaata
     4741 ctatacatac aagtgaattt aatgagtcaa accaagcaag aggaacatca aatcctccaa
     4801 cattcatgtt tacaaatgct gcaccataat cataaactgc aagataagtt agataccaga
     4861 atacccagaa aattacagag aagaatgata taagtataat agcccaaatt ctcttcttat
     4921 ccatgcttgt aagaggagac ttatcttttt cttcaacttt ttcagcaact acaccttctt
     4981 taaaaggtct ctttcctgca tttccaagga atctccatcc atatatgaac catattgcac
     5041 ctattacaca aagtacagct gctataaaga aacattgaga aaatccaagt acaccatttt
     5101 ttgcaaatgt tttaaggtaa agaataccaa ctgctatagt accaataaaa gaacctatat
     5161 taacaaaaga atattgtact gaaaatgctg tatcaagttc ttcttgacta tcaaaaagct
     5221 gtccatttac cgcagatacg ttacctttaa agaatgcagt accaatcgaa actaagatta
     5281 tcattgcatt taccatagaa actgtagttg caatagaacc aagatagtaa cctaccccca
     5341 tgataagcat acctataggt atagtatatc tagctcctat ccatctatct gatatatatc
     5401 ccccaattat aggtgcaagg tatgtgaatg caacaaggtt ggcttgcatt atagcagctt
     5461 gtcctttatc tattccaaga ccaccatgaa ctatagctgt tgtaaggaat atatatatta
     5521 agtactttga agcataatat gctgctcttt caaatgagaa agttgttgag cagatataaa
     5581 atcccatagg aaatttaccc ttctttttta ctgttgagct cataatttta aatcctttct
     5641 taattgtatt tttatttttt acgttttgtc caaattcttt cttttatgaa agatataata
     5701 ttattatttt gtaaaacaat ttgtaattat tattaagata attgcgcatt tatttgtaga
     5761 gtacatattc tcatggaata aaattaatac acttcttaat cataaattta aattatataa
     5821 attcactatt ttcagatata gttataaatt aatatattct catctaatac catgaaaaaa
     5881 ccttcgccat atgcaattct tattttttac gtgatagtat tttacaaact aaaatccccc
     5941 ttttgtagca acttctgtaa ttacatttta gtgctttttt aaaggagtct cgaataaagc
     6001 aacatatatt ttgttcaaat aatgttttta tttacttttt aaatttatat attacttcac
     6061 tcaaccaatt ctcaacttaa ataaataaac tactatctat tcacagatta gacctatttc
     6121 ttacacatta gtttataata aacactgtga atctaaacat aaaaactgtt attttaaagt
     6181 aattacccat atattcgctt tgatactatt tattgaggtt atactcttta tttataatat
     6241 attatttata aaatacctca aaatatatta ttaataaaat acctcaaact gaaaactttt
     6301 tcacgaatat ttatttttca cctccccttt tttattttac catgtaaatc gtaccataaa
     6361 tatgcactta tatgcaagaa aaaaatctat taatcgacag taaataatta gaaaaattta
     6421 aattatattt gttaagtgta ttatataaaa tatttcctat gaaaatataa attaaattaa
     6481 tgttcagaat agtttttaga tatgaaatct tgattttttt attactt
//
LOCUS       DAMJVX010000186         6519 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMJVX010000186 DAMJVX010000000
VERSION     DAMJVX010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.192"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(26..1393)
                     /locus_tag="PC407_003182"
     CDS             complement(26..1393)
                     /locus_tag="PC407_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2498591.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIRYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(1643..2107)
                     /locus_tag="PC407_003183"
     CDS             complement(1643..2107)
                     /locus_tag="PC407_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HDF2498592.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(2112..2741)
                     /locus_tag="PC407_003184"
     CDS             complement(2112..2741)
                     /locus_tag="PC407_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2498593.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADKVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(2775..3662)
                     /locus_tag="PC407_003185"
     CDS             complement(2775..3662)
                     /locus_tag="PC407_003185"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HDF2498594.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYSLYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(3691..4929)
                     /locus_tag="PC407_003186"
     CDS             complement(3691..4929)
                     /locus_tag="PC407_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498595.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYNL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(5313..6455)
                     /locus_tag="PC407_003187"
     CDS             complement(5313..6455)
                     /locus_tag="PC407_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2498596.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
BASE COUNT         2327 a         1085 c          670 g         2437 t
ORIGIN      
        1 tcataaaaaa tttatattgt atatcttatt cttttattgc tttaggtttt gatagaaaac
       61 atttataaaa tataaagaaa gaaattgctg ttatagtagc agctgttata tctgcaactg
      121 gctctgctaa aattactgca aaaagttttt cattaaagaa tgttgggagt ataaatatta
      181 aaggtattaa aagtacaatc tttctaagta gagctaaaac caaagatatt gttgcttttc
      241 caagtgctaa aaatgtctgt tgacaagcta tttgaatccc aaacataaaa gcaccagcaa
      301 aatatatttt tatactccaa ctagtcatac taagtaactg cggattctta ttaaatatac
      361 taacaaaaaa tgcaggaaat atcattaata aaagccataa aatagtagaa tatgtaaaac
      421 atgacaataa acacaactta aatgtctttt ttactctatc aagttgacct gaaccataat
      481 tataacttat tattggttgt gctccttgtg taacccccat attaggaagc ataagaattt
      541 gcattatact gctcattatg gccattgaac ctactgctaa atctcctcca tacatttgta
      601 atttactatt taaggctata agtactaaac tttctgtaga ttgcattata aatggagaga
      661 ttcccaagcc aagcactggt aagatgatac ttgcttttgg tatcatatac ttctttttta
      721 ttttaagtat acttttcttt ccaaagagaa acataagtac ccaaatagca gaaacgcctt
      781 gtgcaattat agtagctaat gcagctcctt ttacacccat attaaagcca aatataaaaa
      841 tcgggtcaag tattatgttt attaaagcac ctatagcaac tgtcatcatt ccagttgtag
      901 caaacccttg ggtatttata aaagtattca tacccattga aatctgaaca aacactgtac
      961 ctattaaata aatatttaaa tagtccattg aatatctaat tgtagattca cttgctccaa
     1021 acatccaaag taaaggctct tttccaatct taaaacctat agtcaaaagt attgctaaaa
     1081 tcaccagtac agaaaaactg ttactcatta ttttctctgc ctcatcattg ttttgctcac
     1141 ccatttttat agcagctaat ggacctcctc ccataccgat taaataacta catgcagtta
     1201 ttataattat tattggaaag gccactccaa caccagccat agcaacttct ccattttcca
     1261 ttctaccaat aaaaatccta tcgactatgt tgtataaaac atttacaatt tgtgcaataa
     1321 tagcaggtgt agcaagttta aataataatt tacccacact ttcactgcct aagttagtgt
     1381 ttgaattttc cattttaaaa cctcctcaat ccaataatta aaatgtatca ctaaaatttt
     1441 attctatata aaaatttcat tttatcagca gacaaattta gagtactttc cctacaagta
     1501 atactatact atatttcttt tgccatagta aacattttag aactaactga atttaaatac
     1561 tttatctcat tttttattta ttattaaaaa tttttcacat gtactcataa aaaaactatc
     1621 caagtatatt acttagatag ttttatatta tatgtaaaga aagagctgaa actataaccc
     1681 ctgataacaa attgccaaga aatactccca taaaagcatc ttttattctc atttttaata
     1741 tggaagctat agcagatgca gtccatgttc ctgtagttgg cagtggtata cctacaaata
     1801 gtattatacc aattatcgaa acacttttta atttcttcat tcttgagttt atttttctat
     1861 caattacatc agcaatccct ttaaatagtt tcttccctct taaaaattgt aatacatgtc
     1921 tgaatgtcaa tatcaaggga atcgatacca atgtagaacc tattacacta aatacataaa
     1981 cccatatagg atttaagccc attgcaatac ctataggaat ggctcccctc aattctgtta
     2041 caggaaccat agataaaatt aatatgtgta aatatcccaa actattccct cctattctaa
     2101 gtgacattaa gttatgaatt tatttgctct ataacctcac catccttttt actaactaaa
     2161 aatgcatttt tagtcaatga aagcattggt aaatcactct tagagtctga atatgcatat
     2221 gaattttcat aatcaatctc tatcccaagt ttacctaata cagcctttat tctttttact
     2281 ttttcaattc ccttacagtt ttctccaatg aattgatttt tatatcttga attttcataa
     2341 aaaaactccg tgcctataac cttgtctgca agaccataat acttaaaata tttcatatat
     2401 gcatatggag atgcagttat cataattata acattatctt cttctttatt tttgtaaatg
     2461 acctttttca tagattcgtt aatttttttc attatgcaat catcaaaaaa cttttttaaa
     2521 tcttcttcac taaaatctac tattggataa aagattgcct cttttgcctt cttcaagggt
     2581 atcattctaa ttaaatacat aaatagaata aaaataatgt atggcaactt tataatagtt
     2641 ttaaaggttt tattttttaa agagtaaata aaaaaatcta tcatgctatc agtatttata
     2701 acagtataat ctacatcaag taataataca ctttttttca taagtatttc cccatcctat
     2761 cactaaaaat atcactaaaa aatcaatatt attaaacatg taattatcca taagattacg
     2821 tttattttta aagcattatc ttttaggaca atatcagtag gattattttc atttgtagtg
     2881 ttaattatat aattatatct aagtacacca taaactacaa atattgttgt aaatatcata
     2941 tgtggctttt ctggattact tgtagaatac aatgaataac atacaattgt agaacttaat
     3001 ataactatca tcatttgatt tagattttct atgtcataat cttccaatat ttttctatgt
     3061 tcaactgcat catctcttag agtttctatt tcttttttac gttttccaag agctaagtac
     3121 agagataaga aaaatgtaca taaaattatc caatttgata aactaacact tatggctaca
     3181 catcctgcat aaaccctaag tataaaacca agagatattg aaaacacatc taatagtatt
     3241 atattcttta gtttaagaga atataatata ttgtttatca aatagagact aagaattata
     3301 gaaagattaa aactaacaac tgttgataaa cagactgaaa ttaaagctaa tactacaaat
     3361 agtatattag cacttttttt gcttattctt ccacttgcca ctggtctatt acatttttta
     3421 ggatgttttt tatctttatc catgtctata gcatcattca gtgcatatac tgatgatgac
     3481 attaggcaaa aacacacaaa cgcaataaac gtatttaaaa ataaatttat atttagtatg
     3541 ttgtttgaaa aaattatagc tgaaaataca aagaaattct ttatatattg ctttattctc
     3601 attaacttta tataatctga aattttagta agaagcctta tacttgtatt ggtctgtttc
     3661 ataaacttac taccctttca atattatttt ttaaacagga tatgatttta tccctatgtt
     3721 taaaatttct ataaatccat tgacattact gattactata gtaaccaata acagcgatag
     3781 aaatatatta tacatattct tttcattttt tctaagcgct ttcgtaaagt aagccaataa
     3841 aattataact gcaaaactaa aatttatagc cataatactt gaattgacta gattatatcc
     3901 tactatacca tgtaaagtta gattgaatat aattattccc atacatagct taggaactct
     3961 tttttctgaa ttttcaataa ctgataaaac acaaaaacat aaagctaata atgtaagaat
     4021 tactggtatt gtagcaactt ctccattttg caaaaataac ccttttttaa tacttgctcc
     4081 aggaaaataa aaaaccgaat acaataatct ctctataatc attgcaatct tttccaaaat
     4141 tggttctcca cttataaatt tcgtatcata ctgtattaca tttccaaggt tcataaaact
     4201 ctcataagac ttcgttgaaa ctacacaaat atatgccatt aggataaatg taattaaatg
     4261 ttttaacatt tttcctattt ttaatttaaa ctcaaatatc atattaaaaa aatacaaata
     4321 tatattacta atagttattc caaatagagg aatagctgct aattctacta aataagaatt
     4381 ttttcctttc atcttatttg caataactat gaaaaatatt aatgaaaatt ggctatatat
     4441 aaatctctct acatttaaag tcataaataa ctgtggaaaa gaaaatatca ttatcatagt
     4501 aagtaaaatt aaagttaatc tctgcatttt tatcttttta atatgattaa agactaaagt
     4561 tattgacata gcactagtta ttatttgtag acaaattagt ccaaacccat aggcactatt
     4621 aaaatctgtt gtcccaggat ttgctattga agtacaaaat aggtatattg gatatgcaaa
     4681 aattgatata attgctgaaa acaatatgtg cttactattg tcttgactta tagcaaatac
     4741 atttaaattg aataaaaccc ctgtatctgt atctaacaat acatcataag tattgtttaa
     4801 attataaaat atagtagtct tagaaaaaaa tatcttagtt attattaagt aagttattac
     4861 aactaatata aacaaaaaca tctcgttttt atattttaat attttactca tcaatatatc
     4921 accatccatt cattatatag gatttgctga cacctacaat ttattcctta tatttaatag
     4981 ccatcttcat aatgttctta cttgttttat tgtaattgaa taactcttta tttacaacca
     5041 caatatttac tttattgatg ttataatcag tcaagtattg tttatataac atagattagt
     5101 gttgtataaa atgagaattt gtaagcaata ttaatgtaaa atatttgaaa aactttatat
     5161 aaaaatatac aatgttgctt atggaattaa actataacaa attaaagcat aatttaagga
     5221 gacaaaatta taatctgtct ccttaaatat taaaactata ttttaaaaca ttactaaata
     5281 ctatacaaat ttcaaatata gtataaaatt aattagactt taacactatt agctccatct
     5341 tcttcccatt ttttaatttc ttttaatcta tctattatag gtaaatgctg agtacattca
     5401 acttcacatt ttccacaaga tatacattct ttagcttttg ccttactatt cattgtgtaa
     5461 ttccaatgtc ctaaaccata aactaacttt gtcatttctt catcactctt tttaaacatc
     5521 tgcttttcat tataaaaaag catataggct ggggtattaa taccttttgg acatacttta
     5581 caataaccac aacctgtaca tatttcgttc atattcttat ttaatctagt ctcaatatca
     5641 tgtatatcct catctgaata aattctattt tcgtctgcaa ttttacatgc atcatctata
     5701 tcttgttttt tattaaaccc tatcaaagat atacttattt gtgaaattcc tatgttgaat
     5761 cttaaagctg attctatagt actttcgcca tcttttgata gaaaagaaag ttctttatta
     5821 tactttggaa tagttccacc acttaatgga ttcattgcca caacaccata tccattatca
     5881 acagcatatt tggcaccttt tagcctatat ggaaagttta atatatttat tcccatagtt
     5941 acaccttcaa acttattttc acttagaata tctataacct catctcctgg ttgatgagat
     6001 gaaaatacta tatggtctat caatccttct tctttacatt tcaataatcc ttcatattga
     6061 cctccaggtc tcattgctag ttcgtaatgt tccatctttc taatattcca aacatgataa
     6121 aaatttattt tagatacgcc taatcgttct attgattttt ttacagcctc tatagctttt
     6181 tctgatgtat cacatgcttt tggcttttct ttagttgata cataaaaatc ggtcttacct
     6241 tcctttatca tttgtctaaa ggctagtcca aatatatctt cacttctgtc atcacaatac
     6301 cctggcgctg tatcaaagta attaatacct ttttcatacg catatttgac taaatcagca
     6361 ttttcttgat tacttttttc taaatcaaac cttaatcctc catagccaac tactgaaacc
     6421 attttccctg tctttccata ttcaacgtat ctcattgtaa attcctcctt agatatatta
     6481 agacttaatt tctttataga aaatattata ccataaata
//
LOCUS       DAMJVX010000187         6457 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMJVX010000187 DAMJVX010000000
VERSION     DAMJVX010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.193"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     misc_binding    102..313
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            476..1765
                     /locus_tag="PC407_003188"
     CDS             476..1765
                     /locus_tag="PC407_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2498597.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1819..2898
                     /locus_tag="PC407_003189"
     CDS             1819..2898
                     /locus_tag="PC407_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HDF2498598.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2919..4463
                     /locus_tag="PC407_003190"
     CDS             2919..4463
                     /locus_tag="PC407_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDF2498599.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4626..5168)
                     /locus_tag="PC407_003191"
     CDS             complement(4626..5168)
                     /locus_tag="PC407_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HDF2498600.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDNKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5316..5600)
                     /locus_tag="PC407_003192"
     CDS             complement(5316..5600)
                     /locus_tag="PC407_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2498601.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5663..6427)
                     /locus_tag="PC407_003193"
     CDS             complement(5663..6427)
                     /locus_tag="PC407_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2498602.1"
                     /translation="MNNYLKFATYQLIGFTSSIPSLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
BASE COUNT         2241 a          802 c         1127 g         2287 t
ORIGIN      
        1 tacatataaa actaaaaaat gtaagtaatt gactttttaa gttgacaaaa tattgcatat
       61 atattatgat ataaatcata aattaactac aaatcaatat tatatattcg tagaagaaga
      121 aagtagcata aaggaatttt acagagagtt agatttggct gagaactaat attggaattt
      181 atgtgaaaag gactttggag aatactattg taattatgtt agaatagtgg tttaattcgc
      241 cttaagaaaa tgagtgagta aaattaattt ttactaataa gagtggtaac acggatttat
      301 attcgtctct tggatttttc caagaggcgt tttttattat aaacttattt cagaaaattt
      361 aaaactatat attaaatgaa aattataaaa atatgatagt taataaattt ataaattaga
      421 aaattgtaaa atatgctaag gtcagtagtt ttacgattta aatggaggga tgaaaatgga
      481 agaagaaaaa attaactatg ctctggaaca gattccagag aatcaaaaac gtggttgggt
      541 agctatgttt tcagttttag tagcaatagg tgtggattta tcatctgtta ttttaggcgc
      601 tgagttggca cagagtatgc caatgaaaca agcaatattg tcagtaattg ttggttcatt
      661 tttttctgca attttgtata ctacatgctc tttggttggt tcatctacaa gtttatctac
      721 atctatgata acaaagtatg tatttggaga agctggagca aaaatatttt ctctagttat
      781 aggggtttct ctattaggtt ggtttggagt tcaagtaggt ttctttgcac aaaatgcaca
      841 gataataata aaagatatat ttaatttaga tgtcagtatg cagattttaa gcctaatagg
      901 tggtctcttg atgatgagta ctgcaattta tggatataag gccatggaga agttgagtgt
      961 gtattcagta ccatttttat tagttcttat gatgctaact atattcttag cttttagagc
     1021 aaatggaatt tcagttgatg ataatatgaa atctactatg acatttgcag gtggagtttc
     1081 tctgtcaatg agtataataa tagttggtgc aattgtatct cctgatataa gtcgttgggc
     1141 taaaagtaga agagactgtg ctctttcaag ctttttggga atacaatttg gaaatgcttt
     1201 tatgataata gtttcgatag tattagtaaa atgtatggga acttcagata ttatgagaat
     1261 ttttattaca ttaggaattg ctataccagg tataatagta cttacattag cacaatggac
     1321 tacaaataca agtaatgtat attcagcatc attaagtata gctcttgtac ttaagaaggc
     1381 tcctgagaaa gtattaacta tagtccttgg tattatagca acattattgg ctgtatttgg
     1441 aatatatgaa ggttttatag gatttttaaa tttacttgga attgtaattg cacctgttgg
     1501 tggtgtatat acagcagaat attacatagt aaagcaagaa cttaaaggct ttgataaagg
     1561 agtattatat aaaccaatag ttaaacgttc aatagtgtct tggataattg gtatattaat
     1621 aacttacctt tcaacatatg gatttataac tcttactact atagcacctt tagatggttt
     1681 tatagcaggg tttgtagtac aatctattat tggaaaagtg ctttgttcaa caaaaaataa
     1741 gcaagaaata gacaaagcag tataaagaaa tttgtttata aagtataaaa taaattagaa
     1801 atatgaggag aaagtaacat gagattttta acagaagaaa gtgtagataa aatagcagtt
     1861 ggtgcagcag tgcttggaac tggaggtgga ggtgacccat atgtaggtaa attggttgca
     1921 aaacaagcca ttaaaaagta tggtcctgta aaagtaataa gtttagatga acttgatgat
     1981 gatgctttgg tagttccagt atctggtatg ggttctcctg taattacaat agaaaaatta
     2041 ctttcagaag tagagttaac taccccttta gaaataatgg agaaattatt aaatagaaaa
     2101 gtggatgtta taattcctat agaaatagga gggataaact cacttatgcc tatagcagtt
     2161 gctgctaaaa aaggacttcc tatcttagat gcagattcta tgggtagagc tttccctgaa
     2221 gctcaaatgg taacttttta tcttgaagga tatgaggcat ctcctgccgt aatgtctgat
     2281 gaaaaaggta attctgccat cctttatcct gtggatggaa tttggtcaga aagattagct
     2341 agaactttaa ctgtagaaat gggaggaagt tcttctatta gtgactataa tttaagtgga
     2401 gctcaagtta aaaaagctgc tataggtaat actcttacaa tagctgaaac tataggaggg
     2461 tttttacttg aaaataaatc tgattcaaaa ggtgctgttg agaatatact aaaagaattg
     2521 tgtggataca agttatttga aggaaaagta attgatatta agagagagtt gaaaggtgga
     2581 tttactagag gacatgcctt ttttgctggg attaatgagt atgatggaga gtattctata
     2641 ttattccaaa atgagaattt aattgccaaa aaaggtgata caccactttg tattacacca
     2701 gatttaatag cagtattaga tttagaaact ggatttccaa taactacaga gagaataaaa
     2761 tatggctctc gtgttatggt agtagctttt ccttgtaatg aaaagtggag aacagaaaaa
     2821 ggaattgaga cagttggacc tggatacttt ggatatgatg tggaatataa aacagtagaa
     2881 gaattacaag gaaaataaaa gtttaggagg aaattttgat gtatcgtgta ggaattgacg
     2941 ttggaggaac aaatacagat gcttgtatac tggatgggga attaaaagta attcattcag
     3001 ttaaggtggc aactacaaaa gatgttgaaa ctggtgttta caatgcctta aaaaaagtta
     3061 tagatgaaag taagattgac cattcattaa taaaatatgc tatgcttggt acaactcatt
     3121 gtacaaatgc tattgtagaa agaaaaagac ttaataatgt agctatgata agaataggaa
     3181 agccggccac tactgccatt cttccattta tagattggcc agaagactta agagaaaaaa
     3241 tagaattaga ttcaatattg gtatctggtg gatatgagtt tgatggtaga aagataaatg
     3301 aactttgtaa agatgaagtt gtagagtttt gcaatagaat aaaaggcaaa gtggaaagtg
     3361 ttgcaataag tggagtattt gctccagtaa ataaacaaca agaagaagaa gttgcaaaat
     3421 gggttagaga aattttaggg gatattccta tatctttatc aaatgaaata ggtaatatag
     3481 gtatattaga aagagaaaat ggagctattt taaattctgc attgtctcaa gtaggtaaag
     3541 ctgtatcttt aggatttgaa aaagcactta aagacttagg tatagatgct caaatatatt
     3601 tcagtcaaaa tgatggaaca ttaatgaatt tagagtatac aatgaaatac ccaattttta
     3661 caattggatg tggaataaca aattctataa gaggagcttc cttcttatca aaagtaaaaa
     3721 atgcaatagt attagatgtt ggtggaacta caagcgactt gggtgtacta tataatggat
     3781 tccctagaga atcatctatt ccagttacaa ttggaggagt acatacaaac tttagaatgc
     3841 cagatgtttt aactattggt cttgctggtg gtacatgtgt aaaaggcgaa aaagataata
     3901 taaagatagg tcctgaaagt gtgggatata gaataactga agatgcaata atatttggtg
     3961 gagacactct aactttatca gatgttgtta caaaattaga tatggcgttt gaagatagaa
     4021 aaagcaatgt tgaaaactta gataaagagt attgtaatga ggtatataag aaggttattt
     4081 taaaattaga agatgctgta gaccaaatga agactagtca agatgatgtt gaattggttt
     4141 taactggtgg aggaagtata attgttcctg aaaaactaaa aggcataagc aatgttataa
     4201 gacctcctca ttatactgct gcaaatgcta ttggtgcagc tttaggacaa ataagtggag
     4261 atgtagaaaa agtatacagt ttagataaaa tgtcgagaga agatgccata gaagatgcaa
     4321 aaaaagaggc tgtaaataaa gctctaaaag ctggtgctaa agaagatact atagaagtat
     4381 taaatataga agatgttcca cttgcatacc ttcctggtaa tgcacttctt ataaaagtaa
     4441 aagttgttgg tgatttaatt taaagaataa atataagtat ttccagaatt atataaatat
     4501 ttctggactc atttttaaac ttattttatc aataaaaaca gcataacttt tgactatatt
     4561 cttataagtt tccgaacagc cgatatatac ctattttttg aggtttatcg gctgtttgtt
     4621 atcttttatc gcattccaac tatttttctt accgtatttg ctgtacgaat tgtaatctta
     4681 tcacttacat ttgaactggc agtctttgac caccaatttg tctttttata atctttacga
     4741 ctaaaagacc aaaagatagc atttttataa tgatatactt tttcatactc ttctttagga
     4801 tttccaacct catcataaaa ctcgtcataa gataagtcag gaaacataaa aataagatta
     4861 tcatatattt ctttgttgtt atctccccac caattaggag cattatttag tatatctttt
     4921 aactcctctt gtgaaataat aaaaactggt atttccaatt caaactgttt ttttatcatt
     4981 aatcttattg tatttgagat aaccttgttg tcatctacat cacttaaaaa gacaacatta
     5041 ccactattaa ggtatgtaga aacttctgaa taaccaagtt ctacaaagct tgcttttaat
     5101 tcagacataa ttatcttgtt cttaccactt atattgatac ctcttaataa tgcgatataa
     5161 cttttcatat aataagttca cctctaaaca ttcacatttt tctatgtagt acaaactgga
     5221 atttgcatct tttataattc tttcaaaact tcttcatatg taattttttt aatataaaaa
     5281 aatcggtgct taatttgtac cgatttttat ttttcttagt aaagaactcg tttttttgct
     5341 tctgctatag ctctagcagc actttttgct tgcattcttt tttcttcttc ttttttaaat
     5401 tcatttattg tttcatttag attcttatca tgttcatttt tttctttatc ttcgttttct
     5461 ctgtatacta atatataatc tattattgat tcgtttatta tctgagatat acttttattt
     5521 tcattaattc ctattctctt aacttcctta agtagagtct catcaatatg aaccgttgtt
     5581 tgctttgtat tttttgccac cttaatcacc tcttaaaaaa aattctaaca ttaaaatttt
     5641 ttttagtcaa taaattttaa tattaaaatt ctttaaatgt tttatttgat tttctaaaat
     5701 tttctattgt atttatcatc ttatttttta tattttcacc tagtacagaa gactttagtc
     5761 taggattttc tatatggtca taaattggtt ctaaaaattc gacttcgaca ggaacagagt
     5821 taattttacc aataggttgg tatccttcat aagtatcttt tgaatttttt attacgagtg
     5881 gaacaatagg gcattttgct ttatatgcta tctttaaagc tccacttcta aactccgaaa
     5941 ccaatgaatt tggttcttta atccatgtta aatctccttc tggaaacact gccatacttt
     6001 gacctgtaag tatattttga gaggcttggg ctatactctt tataccctca cgattgtttt
     6061 ttctgtttac atatacacat cttagtaatt ttgcccattc tttaaatata ggtatatttc
     6121 tccaaactgg ttcatctgca attacacatc caattggtct ttctacactt gctgttagta
     6181 tgaagctgtc aagcatacta gaatgattta caacaaatag aagtggttct tttggcagtg
     6241 tttccttacc aattatatta agctctatgt tcactatatc taaagatttt ttagcttgtt
     6301 tttgcataaa ttcaaatttt tcctttaagc taaatttatc aggattcttt ttaattttaa
     6361 taaggctagg tatagaagat gtaaaaccta taagttgata tgtagcaaat tttaagtaat
     6421 tattcataaa attatctcct taaaataaaa ttattta
//
LOCUS       DAMJVX010000188         6356 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMJVX010000188 DAMJVX010000000
VERSION     DAMJVX010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.194"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            294..1238
                     /locus_tag="PC407_003194"
     CDS             294..1238
                     /locus_tag="PC407_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDF2498603.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1260..1886
                     /locus_tag="PC407_003195"
     CDS             1260..1886
                     /locus_tag="PC407_003195"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDF2498604.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1920..2969
                     /gene="hisC"
                     /locus_tag="PC407_003196"
     CDS             1920..2969
                     /gene="hisC"
                     /locus_tag="PC407_003196"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2498605.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2972..3550
                     /gene="hisB"
                     /locus_tag="PC407_003197"
     CDS             2972..3550
                     /gene="hisB"
                     /locus_tag="PC407_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDF2498606.1"
                     /translation="MRIWKVERNTFETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3552..4157
                     /gene="hisH"
                     /locus_tag="PC407_003198"
     CDS             3552..4157
                     /gene="hisH"
                     /locus_tag="PC407_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDF2498607.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDITLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELVAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4154..4876
                     /gene="hisA"
                     /locus_tag="PC407_003199"
     CDS             4154..4876
                     /gene="hisA"
                     /locus_tag="PC407_003199"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDF2498608.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGINTKIIEDIANNIDIPIQVGGGVRDKGKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSDVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4858..5616
                     /gene="hisF"
                     /locus_tag="PC407_003200"
     CDS             4858..5616
                     /gene="hisF"
                     /locus_tag="PC407_003200"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDF2498609.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKNMGVEVRL"
     gene            5644..6312
                     /locus_tag="PC407_003201"
     CDS             5644..6312
                     /locus_tag="PC407_003201"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDF2498610.1"
                     /translation="MDNKCNNVYSDEVEEFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQKLWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMEPEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2594 a          538 c         1260 g         1964 t
ORIGIN      
        1 tgtaaattta gaatattaat aaaaattatt tgggaggaaa ttatcatggt gagattaaat
       61 atagtaaaat taaatacaaa attaaatcat caccatcatc atagattaaa gggattgtaa
      121 tatgaaatta attcatagac acaagccctg tttttagtgt aattggtctt gtatctatga
      181 tgggaagtaa ttttaggaga aaattatagc catataggta taaagcctat atggcttttt
      241 tattgtttta aatttgcaaa tataagagta aatttaatga agagggtgag aatgtgaaat
      301 ttttgaggat tgaagatgaa gttatttatt ctaaaaaaag atataaatta aaaagggaaa
      361 tagataaact ttttttggat gaagaatatt ttcaaataga accacaatta tttgaagaat
      421 atgatgaatt tacaactata aataataaaa ttccagaaga atctatggta aaggttgtaa
      481 atggaaaagt aatggtactt agagctgata taactacaag tataataaaa agccttgtac
      541 caagatggga agatggttta aagcttaagc ttttttataa ttcatcaata tataaaaata
      601 aaaatacagt tggaataaaa gagataagac aaataggttg cgagtatcta ggagaagcct
      661 ctgtagaagc agatagagaa gtagtgaaat tagctcttaa gatacttgaa aaatacaata
      721 ataattttat tttagaggtt gggagtagca agtatattca tggtttgtta gaagaattaa
      781 atttaaataa gaattgtgaa aatcaaatta aaaatttact atatacgaag aacactcatg
      841 aattaaaagt atatattgaa gaactgaaaa ttaagcgtga agtaaaagag ctattatcaa
      901 atattttaag cttgcaaggg aatttataca acgttattga aaaatcaaac aagttctatt
      961 gtaataataa gatgaagcaa gctcttgaag aattaaagca agtaaataat ttaattgagg
     1021 aatgtaattt tttagataag gctagatttg atttatcaat gattactatg cttgactatt
     1081 atgaaggaat tatgtttaga ggatactacc caaactcata taaagaaatt cttagtggtg
     1141 gaagatatga ttctttaact aaagagtatg gaaaagaaat tccagccatt ggatttacac
     1201 taagtgttga tgagttaatg aagtatgtat ataagtaaag tttagaagga gggattgctt
     1261 tggattattt gactatagca ctagcaaaag gacgaattga aggggaatca tttaaaaaat
     1321 ttaaaaaaat gggtctagga gatagtatag acacagatac gagaaaacta atttttaaag
     1381 atgaagaaaa taaaattata tatatacatg taaagccatc agatgtagtt acatatgttg
     1441 aaaaaggagt cgctgacctt ggtatagcag gtaaagatac tattttagag aatgagacag
     1501 atgtatatga aatatatgat ttaggttttg gaaaatgtaa atttgctgta gcaggattaa
     1561 agggagatag catatataga gaagatgaat atctaaaagt ggcaacaaag tatcccaata
     1621 tagcaaaaaa gtattttaaa gagaaaggtc aaaaaattga aataataaaa ttaaatggtt
     1681 ctgtagaact agctccaata gtagggttgt cagatgtaat tgtagatata gttgagactg
     1741 gcaatacact taaagcaaat ggtcttgaaa tattagaaga catatgtaat ataagtgcta
     1801 gaattatatc aaatagagct agctatagat ttaaatatga acaaattcag aatataattc
     1861 gtttatttga agaacttgat aattaaaata taattgaact ttataaggag ggatttaagt
     1921 tgagagaaaa agaaagtata agagagttaa ggggatatga accaaatcac gtaaactgta
     1981 aagtaaaact tgatgcaaat gaaggaagta agaggttgtt taaatacctt attaaagaaa
     2041 tttcagatag tgatatagat ttaaatttat atcctgaaga ttcttatagt gatttgaaag
     2101 aatctataat agattacata aatatttctg gagtaaataa gaaaaatctt ctagtaggaa
     2161 acggttcaag tgaaataatt gatttaataa tacatacttt tgtagataaa gatgaagtaa
     2221 tattgagttt ctctccaagt tttagtatgt attctatata tagtcagata aatgggtcta
     2281 aatttatagg tgttgaaagt gatgaaaatt tagttataaa tatagataat gtgattgaaa
     2341 aagtaaagga gaataatcct aaaatagtaa ttgtatgtaa tcctaataat ccaactggaa
     2401 caatattaaa gagggaagag ataataaagc ttttagattc aactaatagt ctagtggttt
     2461 tagatgaagc atatatggat tttggagaag aaagcatgtt aagtgatgtt tttaagtatg
     2521 ataatctaat agttcttaga actttatcaa aggcatttgg attggctgga ataagaacag
     2581 gatatatgtt atctaatagt agtttaataa attctgttga gaaagtaaga cctccatata
     2641 atttaaattc attatcagac tttattgcta caagagcact tagaaataaa gatgtggtaa
     2701 aggcttatat aaaggaagta aaagaggaaa gagaagtttt atatgaagaa atgattggta
     2761 tgggaattaa agcatacaaa tcacaagcaa attttatatt attttattct gaaatcgaaa
     2821 atttatcaca aaaacttatt gatagaggag ttttaataag aaaatttggt ggaaagttag
     2881 aaaattatta cagagttaca attggtgata aagaagaaaa ttctatgttt gttggagcta
     2941 taagagatat tttgaaaaag gagaaatagg tatgagaatt tggaaggtag aaagaaatac
     3001 ttttgaaaca cagattttag tagaattaaa tattgatggt tctggtaaag ctgaaataga
     3061 tactggaata ggatttttag accatatgct taccttaatg tcatttcatg gcaagttcga
     3121 tttaaaagtg atttgtaagg gtgatacata tgtagatgac caccatagtg tagaagatat
     3181 aggtatagct ataggtgaag catttaaaaa tgcactaggt gacaaaaaag gaatacgaag
     3241 atattctaat atctatatac ctatggatga atctctatca atggtagcaa tagatataag
     3301 taatagacct tatcttgtat ttaatgctaa atttgatacc caaatgattg gtagtatgag
     3361 tactcaatgc tttaaagaat tttttagagc ttttgtaaat gaatcgagag taacattaca
     3421 tatcaatctc ttatatgggg aaaatgacca tcataagata gaaagtattt ttaaagcatt
     3481 tgcaagagct ttaaaagagg gtagtgaaat agtttctaat gagatagcat cttcaaaggg
     3541 ggttttgtag aatgaatata atagtagact atggtcttgg aaatatagat tctgtgtcta
     3601 ggggttttag aaaagctgga atagaaacta aaatttctag tgatatagat gaaataaaac
     3661 aggcagattc acttatactt ccaggtgttg gagcttttag agattctata agtgccttag
     3721 ataaactagg attgatacca ataataaagg agcatgtttc taagggtaaa tttatgatag
     3781 gtatatgtct aggaatgcaa ttactttatg aaaaaagtta tgagtatgga gaatatgaag
     3841 gattaggact tataaaagga agtattgata aactggatat aactttaaaa gtgccacata
     3901 tgggatggaa taatctaaaa tttaataagg caaatgatga tatacttaaa tatataaacg
     3961 aagatgatta tgtatacttc gttcactcat attatgcaaa ttcatcaaat gaagaactag
     4021 ttgcattttc agaatatgaa aagaaaatcc ctgctattgt tagaaaaggc aatgtatatg
     4081 gtatacaatt tcatcctgaa aaaagtggag aagtagggtt aaatatatta agagcatatg
     4141 gggagatgat aaaatgataa tatttccagc aatagatata aaagataata agtgtgtaag
     4201 actcacacaa ggagaatttg acaaagtaaa tgtatattat gataatccat tagaagtagc
     4261 atataaatgg aaaaatgaag gagcagaata tatacatata gtagatttaa atggtgctag
     4321 aagtgaattt ggtataaata caaaaataat tgaggatata gcaaacaata tagatatacc
     4381 tattcaagtt ggtggtggag ttagagataa gggaaaagtc aaaagtttaa taaatgctgg
     4441 tgttactaga gtaatattag gtagtatagc gattgaaaat ttgaatttgg ttgaagaatt
     4501 ggtcaatgaa tataaagaga aaattgtagt ttctatagac gctaaagatg gaaaagtagc
     4561 agttagggga tgggaagttg taagcgatgt agactcatta acattatgta aacaacttga
     4621 aaagataggt gtacaaacta ttgtttatac agatatatca aaagatggga tgttgcaagg
     4681 gccaaatttt gacatatatg aaagaatagc aaaggaaacg tcattaaatg ttattgcttc
     4741 tggaggcgta acttctatag aagacgtaaa aagactaaaa gctatgaact tatatggtgc
     4801 aataataggt aaagcgcttt atgataaaaa gatagacttt aaggaggcac aacaattatg
     4861 cttactagga gaataatacc ttgtctagat gtgagaaatg gaagagttgt aaagggaaaa
     4921 aaatttaaag atatagtaga tgttgatagt ccagaagtac ttggaaaatt ttatagtgat
     4981 tgtggtgcag atgaacttgt attctatgat ataactgcat caaatgaaga gagaaagaca
     5041 tctttagaat ttgtgacaaa agttgcagaa aatataaata taccattttg tgttggtgga
     5101 ggagtaaata aattagaaga tttcactgat attttaagaa aaggtgctga taaggtttct
     5161 ataaattcat cagcagtcaa aaatcctgaa ttaataaggg aagcttcatt aaaatttgga
     5221 gcacaatgtg ttgttttatc gatagatgca aagaaaaatg aagagggttc atggagcgta
     5281 tacgtaaaag gtggaagaga aaaaactaat ctggatgcaa ttgaatgggc tgttaaaggt
     5341 gtagaacttg gagcaggaga aattgttgta aatagtatgg acgaagatgg aatgaaaaat
     5401 ggatatgata tagaattatt atcaaagata acttctttag taaatgtacc tgtcatagca
     5461 tctggaggcg ctggaaaaaa ggaagatttt tatgaggcag ttaataaatc taatgtagat
     5521 ggaattttag cagcatctgt ttttcatttt ggcgagataa aaataaacga tttaaagaaa
     5581 tatttaaaaa acatgggtgt agaagtaaga ttataagtta aatactaaaa ggagattaga
     5641 aatatggata ataagtgtaa caatgtatac agtgatgaag tagaggaatt tataagaagt
     5701 ataaagtttg atgataaagg gttagtccct gttgttgttc aagaggtagt tagtaaagat
     5761 gtactgatgt tagcatatat gaataaagat gctataaaga aaaccttaaa agataaagta
     5821 gcttgctatt ttagtagaag tagacaaaaa ctttgggtta aaggtgagac atctggtaat
     5881 actcaaaaag tagttaagat gtcttatgat tgtgatgtag atactatact actttttgtt
     5941 gaacaaactg gtgtagcttg tcatacaggc aattattctt gtttttacag agacttgttt
     6001 gatgatagtt caaaaatgga gcctgaagta caaacaaata tattgaaaga attatatgat
     6061 ttaatcaatg aaagaaaaaa taatccagtt gaaggttctt atactaatta tctttttgaa
     6121 aaaggaatag ataagattct taaaaaagta ggtgaagaga gtagtgaggt aattatagca
     6181 tcaaaaaata ccgataaaag tgaacttatt tacgaaatta gcgatttagt gtatcataca
     6241 ttggttttaa tgatagaaaa aggtgttgaa attgatgaaa taaaaaaaga attacttaaa
     6301 agaagaaaat agaataggat agattaatgg taataagaaa atataccgct tttaat
//
LOCUS       DAMJVX010000189         6244 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.195, whole genome shotgun sequence.
ACCESSION   DAMJVX010000189 DAMJVX010000000
VERSION     DAMJVX010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.195"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(<1..114)
                     /locus_tag="PC407_003202"
     CDS             complement(<1..114)
                     /locus_tag="PC407_003202"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2498611.1"
                     /translation="MSKKHKKYSKELKLKAVNLYIKEGYSSYKIAEMLNIRS"
     gene            complement(431..940)
                     /locus_tag="PC407_003203"
     CDS             complement(431..940)
                     /locus_tag="PC407_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF2498612.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(942..1589)
                     /locus_tag="PC407_003204"
     CDS             complement(942..1589)
                     /locus_tag="PC407_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498613.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(1603..2775)
                     /locus_tag="PC407_003205"
     CDS             complement(1603..2775)
                     /locus_tag="PC407_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2498614.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNIESYTHCKESVNKLAE
                     RLKLEFEKEGLDCELVDVGENGNTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGALCAFNMETGLVDNSLCIGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSRENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            3043..4419
                     /locus_tag="PC407_003206"
     CDS             3043..4419
                     /locus_tag="PC407_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498615.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEILNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            complement(4670..5503)
                     /locus_tag="PC407_003207"
     CDS             complement(4670..5503)
                     /locus_tag="PC407_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2498616.1"
                     /translation="MTIKEVEEKTGLTRSNIRFYENENLIKPLRNERNGYREYSDENV
                     EDIKKIAYLRTLGITIDDIRNIILQKVSLVEVVEKQEKVLETKISDLENAKTMCNKML
                     ESDELSYLNLKVEQYITELPEYWNKNKPVFKLDSVSFLYIWGGKITWGIITIACLLIA
                     ITVFPKLPPQIPIQWNNGVANYVDKKFIYAYPVICVVFRILLRPFIWRWLQIHAFYSD
                     TIADYLTNFMCFVALSIEIFSILFIYNIARHVTIIIFIDILVFIVLLIIGVYKLFKKY
                     N"
     gene            complement(5832..>6244)
                     /locus_tag="PC407_003208"
     CDS             complement(5832..>6244)
                     /locus_tag="PC407_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2498617.1"
                     /translation="GIDYENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMG
                     ASKKGSYGASIIEKIESIIPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQ
                     QLNNHEESQSHPDEQEIYEASMFAKNMMIKASIV"
BASE COUNT         2167 a         1060 c          673 g         2344 t
ORIGIN      
        1 gcttcttata ttcagcatct cagcaatttt gtatgatgag tatccttctt ttatatataa
       61 atttacagct tttagtttaa gttctttgct atatttttta tgttttttag acatagaaaa
      121 acccccttag agtatacgct ttttagactt attataatag ccttttattt aaatgtctac
      181 tataagggga ttatactaaa atagagataa gcctctcttt cattgttgaa attatgaaaa
      241 tatacttgat ttaataccaa aagagtatct catgaactaa aaagttcatt ttgagatact
      301 ctcttctttt ttaattaata tacatattaa ttactatatt agtttattac tatatatcaa
      361 ttactattca ttaattacta ttcatcaatt cttatatatc atttataatt aggttataat
      421 tttatgttat ttacattaat cccataacag tgagtaaaac tcttactatc aacatcccca
      481 ctattgcatc aaatatagga actgccaaca gtaacattct atacttagta ctggcatttg
      541 ctacaagcac tattctggca taatgaccaa ctagagtacc cattaacata catggcataa
      601 ttaatatggt tgcttgtggt gcagtgatta gaccttgtgc aaataagttt gcagctgtag
      661 ctgctcctgc tgccttagca aagaatgctg ctatcaaaac tacaacagat tccccaggta
      721 gtccaaaaat acccattact ggaccaaaaa tatttcctaa aatatcaact aaaccagtta
      781 attgtaaaaa ctgcactatt acataaccca ttatcatggc tggtaatatc tgttcaacac
      841 ctatgtaaaa accttttttt gctcccccca taaatatctc aacagcagtt ttcttaggtt
      901 tactatttac aattgcattt ccagtatttt gttgactcat actacacagc ctccttagcc
      961 ttattctttt tatttcttac acctatgatt aatctaacta gattagcacc aagtatttta
     1021 cagaatatct ctataaggat tattggacct aatgccaata atgctattgg taaaagtgga
     1081 gcttgagtat ttattgtgtt cagtataaca gctgaaccag catattgata tgctacaaat
     1141 atagttcttt catcatctgt tatttcccca ctttcaaata attctttcgt cattattgag
     1201 gctacatctg aactagtaaa tgaagataca aatgctattc ctgctattcc aggtatacca
     1261 agcagaggtc taagtaatgg attaaatatc tttgatgctg ctttcattcc tcccatagat
     1321 tcaactacat ctataagacc tacagcaaaa cagacagttg gaatcaaagt cagagcaaat
     1381 aagaaaccat cttttgctcc cgttcctcct ttacccataa agtctacacc atctgcaata
     1441 gtaccaaatg aacctgtcag attagcaaaa tctaatgctt taaggaagcc ttgactatct
     1501 ttaaatactc ctgaaaataa tactatcaat acaaataaag ataaataccc tttccatgtt
     1561 accttattat ctccagctgt tacatccata taaaatcccc ctttatacct caaatttatc
     1621 taagtttaaa ataactgtag aaattaattt caccctctca aacatagaat ctactactgc
     1681 atattctctt gatgtgtggt tccattctcc tcttacacca aatgaacaga ttgtaggaac
     1741 gtttgcaata gttaaataac ttgcatctga actacctcca agtctcttag catttaattc
     1801 tccaaatcca ttttctcttg acacttcatt tacaaatgta tgaaatctca taacatcttc
     1861 tgtagtttca aatggcatca tttcacttat aaaattaact tctgtagaag ttccttctat
     1921 gtaagtttct ttacatatct ctctaacttg ttctttaaca ttctccattt cttctacttt
     1981 ttcaaatcta aggtctactt ttatactaca atcttctgga atagaatttt ccactcttcc
     2041 acccttaatc acacttacac ttactgtagt tccaacatct aaattagtca atttttgtat
     2101 gcgtagtatc ttattagcca tttcttctat agcatttctt ccaccttcaa aatcattacc
     2161 tgcatgtgtt tctacacctt ttacatgtat attacatcct attcttcctt ttctacctat
     2221 acataaacta ttatctacta atccagtttc catattaaaa gcacaaagag caccttttgc
     2281 ttccctcaat ataacatctg caccagttga atctttatga cctatctctt catctccaga
     2341 aaatactatt tttattggtc tttctttgta tcctatttta tttaaagctt taataacgta
     2401 taaagagatt attatacccc ccttcatatc taaaacacct gggccatatg cttttccttc
     2461 tattatttta aatggattct ctccaaatgt acctgtttca aagacagtat ccatgtgtcc
     2521 tgaaaaaatt atcggctttt tatctatatt agaaccaagt gtacctatca atgtattacc
     2581 attttcccca acatctacta gttcacaatc caaaccttct ttttcaaatt caagctttaa
     2641 tctctcagct aacttgttta cactttcttt acaatgtgta taactttcta tattaacaat
     2701 ctctttccat aatgaaacta tttcttctct attctcatca acaaacttag aaatttcatt
     2761 taaatatgta ttcataatct tctcctttat tttcaaataa aatgtatttc tttcctaaaa
     2821 tctaagttta ttttaggaaa tcttaaaaga ctagtcctta attacaaaat agcacaatta
     2881 ttcttgccag tcaacatatt tttcagtctt ttttatattt tctgattttt ctttcaatta
     2941 tccttttgta aaataaattg acgcgtttta tctactatta taaaatgagg ttataaattg
     3001 attgattagt tacaatactc tcgaaatttt aaaggaggtt ttatgaaata taaaattggt
     3061 tttttgacaa catcttattt tatgtcagat aagtttaaac aagctcttaa agaattaaat
     3121 gatacttgtg aaattacatt tattgagctt aaaggggaga atccttttaa taatttacct
     3181 aatatatact atgagcaagt agataatttt gatggatttg tatgcagtgg tatagttcca
     3241 tatagtgaac ttataataaa tataaaagac ataaaagttc cattaaattt tttaaagctt
     3301 gatgaaagag atttttataa atacttattc aaattattaa atagccaaaa agatattgac
     3361 tttacaaaat catttatgga ttttttaaga gaggacaaca attattatga tatatattca
     3421 ttaatagact ctaataaatg tccatataca attaaagatt ttaatatccc tcaacctgta
     3481 tatgacttta aaaaattaaa tgataaatta ttggaaatac atttagactt attgcgagat
     3541 aataagatta acctttcttt tactagaaat tacatcatta gttgtgaact taataagctt
     3601 ggatatgatt gtatttacat ggttccatct acagaatcaa tcctgaatac atttaaatct
     3661 ctggtgaatg aaataacatt acaaaactta gacaaaaata aatctgcaac ttgtatagtt
     3721 acagtaaata gttcagagta cataaatgaa gatttgatat ttaaaaatga tgagatacaa
     3781 aatcaaatat ataacactat attaaattct ctatcaagac atggattcta tggagttcag
     3841 gtcaaaaaaa atgatatgaa aatagagata cacactacta aagaaatatt aaacaatatg
     3901 accaataact atacagattt ttttatttca gaagatttaa aagaaattaa atatagctta
     3961 aatataggtt ggggcattgg aaatacaaat attcatgcag agcaaaatgc tcgtcaggct
     4021 aatggtaaat ctgcttcttt gaatggaaac tgtactttta tagtaaatga tactaatgat
     4081 acaataggcc ctttatattc aaataaaaat tctaataata ttgaagatag ctcaatagcc
     4141 aataaagttg cttcttttat acctctatct aatacaaatg tatctaaaat catgtgtatg
     4201 attaatgata gaaattcaaa taatgtgtca gcagaaatat tagcagacta tatgaacata
     4261 actcttagaa gtgctaatag aatattgagt atcttatata aagcaggaat tgcaactatt
     4321 gtaaatacaa aactcgataa tcaaagagga aggccaaaaa aaatatataa agttgatttc
     4381 ttatcttttc taaataagat gcagaaatta aattcttaat atttaatttt ttaataaaag
     4441 caatgtattt tctctaattt taaagaatca aaatgtttaa attttattag aatatacaac
     4501 tttttacttt aaaataccta aaccttttat cagtgaaata aaccataata tatcgtagct
     4561 tattagaaaa acaaaataat ttcatataca aataataaaa tagccactac aatccataat
     4621 ttgcagtggc tactttttac actatcactt attacttaat ttttaaaaat taattgtatt
     4681 ttttaaataa tttatacaca cctattatca gtaaaacaat aaatacaagt atatctataa
     4741 aaataattat tgtaacatgt ctggcaatat tgtaaataaa aagtatagag aatatttcta
     4801 tagacaacgc tacaaaacac ataaaattcg ttaaataatc tgcaatagta tcactataaa
     4861 aagcatgtat ttgtaaccat ctccatataa atggacgtaa aaggattctg aatacaacac
     4921 aaatgacagg gtaggcataa ataaattttt tatctacata attagctaca ccattattcc
     4981 attggatagg aatttgaggt ggcaactttg gaaatactgt tattgcaatc aacaaacaag
     5041 caatagtaat aattccccag gtaattttcc ctccccatat atataagaaa cttacagagt
     5101 caagtttaaa aacaggtttg tttttattcc agtattctgg caactcagtt atgtattgct
     5161 ctactttcaa attcaaataa cttagctcat cagattctaa cattttatta cacatagttt
     5221 ttgcattttc taagtctgat attttagttt cgagaacttt ctcctgtttc tctacaactt
     5281 caactaaaga tactttctgt aatataatat tacggatatc atcaatagta attcctagtg
     5341 tacgtaaata tgcaattttt ttgatgtcct ctacattttc atctgaatat tctctatagc
     5401 cattacgctc attacgaagg ggtttaatta aattctcgtt ctcataaaaa cgaatattag
     5461 agcgtgtcaa tcctgttttt tcttcaactt ctttaattgt cattttaaac actacctctc
     5521 tattctatta atttacaact ataatatagt cttaaatatt ttcactctat aaaattgtat
     5581 atttagtata tcttaaaatc aagttaatta tcttttataa attttcatac aataatatct
     5641 cctttcaata atatctcttt ttctatattt aaaactatct tattttctat aaaataatgt
     5701 ataaacaagg tttacagtca agtattttta ttattttttt gatttcataa aaaaaagcat
     5761 agcaaatcat ttgctatact tttttactaa attatttatt ctatatctta tatgaactta
     5821 ttgtttaact actacacaat actagcttta atcatcatat tttttgcaaa catactggct
     5881 tcatatattt cttgttcatc tggatgactt tgagattcct catggttatt taattgttgg
     5941 cgaatatgtt cgttgtcagg agaaagtttt aacatctctt tatattttgc tttcaatcct
     6001 tctgctatct taccttgaca tatgaatgaa cctaatattt cattatcttt tgggataatt
     6061 gattctatct tttctattat actagctcca taacttcctt ttttacttgc tcccattgtt
     6121 ccaaatagtg ctatcttttt attgtgaata ttttcaagca cttgttttga atttttatca
     6181 caaattccct tgtccaccca atagcctaag aatactatat cataattttc atagtcaata
     6241 ccat
//
LOCUS       DAMJVX010000190         6239 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMJVX010000190 DAMJVX010000000
VERSION     DAMJVX010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.196"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            112..657
                     /locus_tag="PC407_003209"
     CDS             112..657
                     /locus_tag="PC407_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF2498618.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            670..1683
                     /locus_tag="PC407_003210"
     CDS             670..1683
                     /locus_tag="PC407_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF2498619.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            1905..3878
                     /locus_tag="PC407_003211"
     CDS             1905..3878
                     /locus_tag="PC407_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2498620.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKENNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            3947..4306
                     /locus_tag="PC407_003212"
     CDS             3947..4306
                     /locus_tag="PC407_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498621.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            4341..4697
                     /locus_tag="PC407_003213"
     CDS             4341..4697
                     /locus_tag="PC407_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF2498622.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            4734..6026
                     /locus_tag="PC407_003214"
     CDS             4734..6026
                     /locus_tag="PC407_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2498623.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
BASE COUNT         2467 a          584 c         1184 g         2004 t
ORIGIN      
        1 aaaaacattt acaatattag catatagtac agaaaaaaga tgtaatttgt aaaatttatg
       61 ttaagcagta aaattaaaaa tttaaagata ttatagggag gttataattt tatggaaaac
      121 gttattacag gtctaagtaa aggtgcagag tggtttatcg gtttgtttca gaaaggggga
      181 gaacaattta taggtcttgt ttcaggtact cttccaacgc taattgtatt aatggtagct
      241 attaactctt tgattaagat aattggtgag gaaagagtta ataattgggc ttctaagcta
      301 ggaaagaact cttttacaag atatattcta cttccattag tatcagtatt tttcttggga
      361 aatcctatgt gctatacatt tggtagattt ttaaaagaag agcacaaagc aggattttat
      421 gatgcagcag tttcatttgt acatccaatt acaggtcttt ttccacatgc aaatgcagga
      481 gaactttttg tatggttggg aatatcagca ggtttgacta ctttaggtaa agaaactact
      541 acactggcat tgtggtattt tatagttggt ctaattgtaa tttttataag aggtatagta
      601 acagaaaaaa tgtatgcttt tctaacaaga aaaaatagag caacaaataa ggcttaggag
      661 gtagataaaa tggaggagaa taaaatatta aaaatagaaa agggaacttc aggctgggga
      721 ggtcctttgt atataaaaaa agaagggaat agaaataaga tactatcaat gactgctggt
      781 ggtatccatg aagtaacatt aaaaataaaa gaactactgg gttgtgaaat tgtagatgga
      841 tttaaaactg gagtttctga tgaagaagta gcagtagtta ttattgattg tggtggaact
      901 gctagatgtg gagtttatcc aaagaaaaaa atacctacaa taaatgtaaa tccagttgga
      961 aaaacaggtc ctttagcaaa atttataaca gaagaatatt atgtatcaga tgttaatcct
     1021 aactgtataa gtgttgtaga tggggaagat atgccacaaa aatctcaaga aaataaaagc
     1081 gaaaataaga gttctattag aaagcctgat aattatgatg aggttaagtc aaaagcacaa
     1141 ggtgaatatg ctaaaaagaa cataatactt tctattggtc aaggggcagg gcaagtagta
     1201 tcaaaatttt atgatgcagg tcgagacact atccaaatgg taatgaataa cgtaatacca
     1261 tttatggcat ttgtaagtat gcttatggga attattttag catctggact gggagactgg
     1321 attgcaagag ttatatctcc acttgcaggt aatattggtg gattattgat tatatcagta
     1381 atttgcacac ttccattctt atcaccaata ctaggacctg gtgcagttat agctcaagta
     1441 gtagggactc ttgtaggtac acaaattggt cttggtgcaa taccagcata tttagcactt
     1501 ccagctttat ttgcaataaa tgggcaagct ggatgtgatt ttgtaccagt aggattaagt
     1561 ttgggagaag cagaaccaga aactgtagag tatggtgttc cagctttatt ttactctcgt
     1621 ttaataactg gtccaatagc tgttattatt gcatatggag ttgcagtatt tgcattgaga
     1681 taatatttaa tgtcaatata aggcttggaa ttaattaata aaattgtaaa tataaattta
     1741 agctagtcaa gattttttgt taatgaaaga ctagctttta tactattaat tagctgtaat
     1801 tatcgagtta gattaatact aaattttcct ttattaaagt gaaattttat gtattaattt
     1861 ggtttcaaat ttgtactaga atgaaatcaa ggaggagata caaaatgagt gtaactgaga
     1921 aaattccttc tattgaccgc aagatattta agatattgat tatgtgtagt aaaaaagagt
     1981 ttgtgagtat taattcaata gcaaatgaat tgaatgtcac aactagaagt gtaaggacgt
     2041 atataaaaca attgaataaa gatttaggta atgatatagc agtaatcaaa tatataaagg
     2101 gtcaaggcta taaactagaa ataaaagatg agcagatact caataagatt attgatatga
     2161 atagaaaaaa tgtattttca ttaaattcaa aagaagatag agtagagttt atactaaatt
     2221 acttgataga actagatgga tttattacac ttgatagttt agctgatgag atgtgtgtag
     2281 gaagaactac tttagtaaat gatttccagt atgtagaaaa agttttggct tcatataatt
     2341 taaacttgat taaaaaacag aatacaggta tgaaattaaa tggaaatgaa cttgatatta
     2401 gactgtttat attaaatcag ttgtataaaa actcaaggaa agatttcagt aattcaaagt
     2461 attttaaagg aatcaaaaaa gaagaaatca taaatttaga agaaaaacta ttgactttat
     2521 ttaaagaaaa taatttttat gttacagatg aaatgttaag agaagttatt aactacatag
     2581 tagttttagt ctatagagta aaagaagaaa agaaagtaaa agactatgat gtaaagtttg
     2641 atttacttaa atcatatgat gaatacttta ttgctaggga aataaaacat ataatatcag
     2701 gaatgtttga atgtacttta aatgatgaag aaattattta tttaactata ccattagtta
     2761 gtggaaatgc accagcatca gaatgtgctt taaatagtag tagaattagt aaaaacattg
     2821 atgagttgat ggaaaatata tttaatcaaa tatatgtcga tatgggtatt tttataaatg
     2881 aggatgaatt aagagtaggt ctaggatatc atcttagttt tactttaaac aggctattat
     2941 ttaatataaa gttaaaaaat gttttgttag aagagattaa gcaaaattat atacttccat
     3001 ttaaattagc acaaatagca ggaaaagtaa tagagaaaaa gtataactta gaagtttcag
     3061 aagatgagat tggatatata gcaatccatt tcagtggata tttagaaaga aatagcagta
     3121 gattctattc aataaaaaaa atagcaatta tatgtagtac aggtctagga acagcaaaac
     3181 tattgaaaat aagagtagaa aaactgatag gaaataatcc taaaattgat actctttcaa
     3241 gttttaattt gagaaatatt aacttagatg aatacgatat agtttttaca actattgatt
     3301 tagacacatc gaatataaat acaatagtat taaaaataaa tacaatattt gatgaaaaca
     3361 aattaagaga acaattaaaa acagtattgt gcctaagaga agggaatatt gacactacta
     3421 attctacaaa tttactaata aataatttgt tagatgaaga taaatttatg atattaaatg
     3481 aaaagactat acttgcttca ttagaaaaaa tgatggacca tcttatggat ttgggatgta
     3541 ttgatgataa gtttagaaaa aatatagtca atagagaaga aaaatctccc acagtatttg
     3601 ataaaggttt attatttcct cactctgtaa atgaaaaatc agataagttt ttaatggcag
     3661 ttggaatttt agaagaacca atagaatatg caaatagaag tataaaaata attcttatga
     3721 caatgtttcc atgtgagaat aagatagact cagacttatt agtaaaaatt tatgaagaag
     3781 ttttaaagat aggccaagat gtaaaattaa cgaataagat aagtaaatgt agaacttttt
     3841 tagaatttaa aaaggtgtta cttaaaaacc ttatataact attgtacagc atttatagtt
     3901 gttatacaga tttaaaaata atgtgatttt tggaaggtgt ataaatatga aattattagt
     3961 aattttattt gttgcttata ttttaaatac tatatttata atgaagcaga ataaattgta
     4021 ttttaaaact ttaaatagag ctaaaaaaaa gggtgatata gtgtctacag gcaaaaagaa
     4081 aagctatttt tctaaaggaa gtatagccat tatatcttca gattcagatg ggtttataaa
     4141 gtttggagaa attttaaaag ggcgtacagt aatggctaaa tttaaagaga ttgaagaaat
     4201 agaaggatta gatatatata gtgcagagca aaaatttaaa gatgaagaga gtattgtaca
     4261 agctataaaa tttataaaag aaaaaataaa tgtttctttt aattaactca atataaatat
     4321 aaattatcag gaggaatgtc atgaaatatg aagttaagat aagtgggata ggcagtttag
     4381 ttaaagagtt aatggatgaa agcaattgtc ttattatata tgatgaaacc attaatgatg
     4441 acgacttaaa ggatatatct gtaattcatt cgatagcaac attaaaaagt gatgttgaaa
     4501 taggagatac acttactata gggaatagag attattgtat agtttcagtt ggtgatattg
     4561 ctcagaagac tcttagagaa attgggcatt gtacaatcaa atttgatgga aaatgtgaag
     4621 taaatttacc aggagaaata catgtggaaa taggaaatcc agatataact attggagatt
     4681 taataactat ttcttaattt gaaaataaaa ttattataac aggaggaatt ggtatgttag
     4741 atttaaacag aaaacttgca aaattggaag aggaaggtaa aaaaataaaa gtagctctta
     4801 taggagctgg gcatatggga aatggaatgg taagccaaat ggcaaatatg aaaggtattg
     4861 aagcttctat tgttgtagat ataaatttgg agttggctca taaagctttt actgatgcag
     4921 gaatagatga agaaattata gataatgtaa caaatgaaaa ggatgcagaa ataaaattac
     4981 aagaaggaaa agtattgaca tgtaatgact ttcttgtggc ttgtaaaact aagtcaatag
     5041 atgtagttat agatgcaact gggggtattg caattggtgc agagatagca ttaaactcta
     5101 ttttaaataa aaaacatata gttatgttaa atgctgaaac tgattgtgtt gttggaccaa
     5161 tacttaaaaa attagcagat gatgctggag tgatatttac aggttctgca ggagatgagc
     5221 caggtgcagt aatggaatta tttgattttg cagatgcaat ggggtttgaa gttagagtga
     5281 taggtaaagg taaaaataat aaattagatt tagattgtaa tcctgataca gttagagaag
     5341 aagcagaaag aaaaggagct tcacctcata tgatagcttc ttttaaagaa ggaactaaaa
     5401 caatggtaga aatggcttta atgtgtaatg caactggatt tgtgcctgat gtaagaggtg
     5461 gtcatggtat tgaagctaca gttaatgaag tacctaagaa atatgcatta aaaagcgaag
     5521 gtggagtatt agataattat ggtgtagttg attttgtaaa tggtatagca cctggagttt
     5581 tcgtagtagt agcccataaa ttaaaagcag ttaatgatga actaaaatat ttaagtatgg
     5641 gtgatggacc taattacata ttatatagac catatcattt atgtagttta gagactcctt
     5701 tatcagcagc tatggcagtc ttagaccata aggctacaat agtacctaaa gctggactag
     5761 tagcagaagt tatgactata gctaagaaag atttgaaaaa aggtgagttt atggatggtt
     5821 atggagcata tacatgctat ggtactatag aaaaatacga tgtagcaaag gctatgaatg
     5881 ctgtacctat tggtttgata agtaaaaaga ctaaagttgt aaaagatatt aaaaaaggtg
     5941 aagttataac ttacgatatg gtagagatag aaaaagatac tactttatat catttaagac
     6001 aattgcaaga aaaaatattt gggtaagatt tgatggtaat ataaatagat attagtaaaa
     6061 agggttgatg gttattttta aatcaaccct tttaaatttt tatatattct tgttaggata
     6121 ctttctttaa aagtatatta taggttattt gtagtcgtaa aaattatttt gaattagttc
     6181 tgcaatttta tattcacatt gaaacaatat tattaaaaat tttaatataa aatgaatta
//
LOCUS       DAMJVX010000191         6226 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMJVX010000191 DAMJVX010000000
VERSION     DAMJVX010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6226
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.197"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            217..1239
                     /locus_tag="PC407_003215"
     CDS             217..1239
                     /locus_tag="PC407_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2498624.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            1299..2306
                     /gene="gap"
                     /locus_tag="PC407_003216"
     CDS             1299..2306
                     /gene="gap"
                     /locus_tag="PC407_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2498625.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            2528..3730
                     /locus_tag="PC407_003217"
     CDS             2528..3730
                     /locus_tag="PC407_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDF2498626.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKANNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            3756..4499
                     /locus_tag="PC407_003218"
     CDS             3756..4499
                     /locus_tag="PC407_003218"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDF2498627.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLSK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            4508..6040
                     /locus_tag="PC407_003219"
     CDS             4508..6040
                     /locus_tag="PC407_003219"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDF2498628.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFANKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         2483 a          728 c         1253 g         1762 t
ORIGIN      
        1 tataaaaaaa tatatataaa aagccttata taaaaagaaa aaatactgtg atttgaaaat
       61 ggtggagaaa tattttataa aataaaaaaa taaaaaagtt tttgaaaaac tattgaaatc
      121 tatacgcaaa tcctataaaa taaaaatatc ttgtgggact taaataaaca ctagggacaa
      181 aaaaagtccc gaaaggaaag caataaggag tatgttatga agaacttatt agaaattcaa
      241 aagaaattga ttccacaggc aattgaactt atggaaagaa gatactctat tttaagacaa
      301 atatctttaa gtgaaccaat aggtaggaga acattatcaa atgtattaga gattagtgaa
      361 agaataatca ggtcagaaac tgagttttta aaagaacaag gacttataga cgtagctgta
      421 tctgggatga ctatcactaa agaaggttct gatttgttag ataaacttaa ggacatcatg
      481 agtgacatca tgggattgtc taagttacaa gatagagtca gagaaaagct tggaataaaa
      541 agggtaatac tagttcctgg aagctgtgat gaaaatggaa gtttactaaa agatgtaact
      601 agatatggat gcgagtattt cttaggcatt ttaaaagatg gagatatcgt ttcaataaca
      661 ggaggaagca ctatgttgga gttttcaaac gttattaaaa ctgataagag atatccaaac
      721 tctacaatag ttccagctag aggaagtatg ggaactgatg tagagatgca atctaacaat
      781 atagttgctt caataagtaa gaaattacat tctaattgca aacttttaca tataccagat
      841 gagttggatg aaagtgcaat gaaaactctt agccaagtgc cagagattaa gacaactttg
      901 gagtatatta aaagaacaga cattttagtt tttagtatag gtagagcaga tgtcatggca
      961 aagagaagaa aacttccaga agacaaggtt gatgagatat tatctaaaaa tgcagttgga
     1021 gaagcatttg gatattattt caacaaagat ggagaaatag tttacaaact gaacactgtt
     1081 ggaattgatt tagaaacagt taaacaagta aaagaaacta ttgcaatctt tgcagggact
     1141 gaaaaagtag aagccttatt agctatatca aatatgcata aaaacatggt tcttgtaaca
     1201 gatgaagaaa gtgcttataa gatattgtca ttattataaa agtcaagacc tagcacagag
     1261 ctagaaaaca tacatttact ttataggagg tagcgaaaat ggttaaagta gctataaatg
     1321 gatttggaag aataggaaga ttagcattaa gaaaaatgat ggagcaacaa gataagttcg
     1381 aagttgttgc aataaacgac ttaacagacg ctaaaatgtt agctcattta tttaaatatg
     1441 atacagctca aggaagattt aacggagaaa tagaagttaa agaaggtgct tttgtagtaa
     1501 acggaaaaga aataaaagta acagcagaaa gaaatcctgc tgacctacca tgggctgaat
     1561 taggagtaga tatagtatta gaatgtacag gattctttac atctaaagat aaagctgaag
     1621 ctcatataca agcaggggct aagaaagttg ttatatctgc accagctact ggagatttaa
     1681 aaacaatagt attcaacaca aacagtgaca tattagatgg ttctgaaaca gttatatcag
     1741 gagcttcttg tactactaac tgtttagctc caatggctaa agtattaaat gataaatacg
     1801 gtatagaaaa aggattaatg actactatac atgcatacac taatgaccaa aatactttag
     1861 atggtcctca tccaaaaggt gatttaagaa gagctagagc tgctgctgga aacatagttc
     1921 caaacactac aggagctgca aaagcaatag gcttagtaat accttcatta aaaggaaaat
     1981 tagatggggc agcacaaaga gtaccagttg taactggttc aataactgaa ttagtttgta
     2041 cattaggaaa aaatgttaca gttgaagaaa taaatgcagc tatgaaagaa gcttcaaacg
     2101 aatctttcgg atatactgaa gaaatgttag tatcttctga cataatcgga ataagctacg
     2161 gttcattatt tgacgctact caaacaaaag ttatggaagt agatggaaaa caattagtta
     2221 aagttgtttc ttggtatgat aatgaaatgt cttatacttc tcaattaata agaactttag
     2281 gatactttgc acaattagca aaatagtcag ttttgagaaa atagaaaagt agattgacaa
     2341 agtccgagtt tgtagtttaa tactacggac tcaggacttt ttttaagaaa aaatatataa
     2401 aatacaccta aataatgtta catactatat ataaaaatat gataatatga tatatttaat
     2461 agggtaaaag tatgtactat attatacttg gcaataaaaa attactttat taaaaggaga
     2521 gatatttatg tcaatgctta acaaaaaaac tatagaagat attgatgtgt gcggaaaaaa
     2581 agtattagta agatgtgact ttaatgtgcc attacaagat ggtgtaatta ctgatgaaaa
     2641 tagattgaac ggagctttac caactattca atatttaatt tctaaagggg caaaagttat
     2701 attatgctct cacttaggaa agccaaaagg agaagctaaa ccagaattat ctttagcacc
     2761 agtagcaaag agattatctg agatgttagg aaaagaagtt gtatttgcag cagacgataa
     2821 tgttgtagga gaaaatgcaa agaaagcaac tgaaaaaatg gaaaacggag atgtagtatt
     2881 attagaaaat actagatata gaaaagaaga aactaaaaac gaagagaatt tctctaaaga
     2941 actagcatca cttgctgaaa tatttgtaaa tgatgcattt ggaactgctc atagagcaca
     3001 ttgctcaaca gttggtgcag gcgagttttt acaagagaga gtttgtggat acttaattca
     3061 aaaagaatta aaattcttag gagaagctgt tgcaaaccct gtaagaccat ttacagctat
     3121 acttggagga gctaaagttt ctgataaatt agctgttata aacgaattat tagaaaaagt
     3181 tgataatcta ataataggtg gaggaatggc ttatacattc ttaaaagctc agggttatga
     3241 agtaggaact tctttacttg aaatagataa agttgaatat gctaaagaaa tgatggaaaa
     3301 agctaataat aagggtgtta acttattatt accagtagac gttgttatgg cagaccactt
     3361 tgcaccagat gctactccaa tagtaacaga agatgctaat gtaaaagaag attacatggg
     3421 actagatatg ggacctaaga ctatagcaaa ctttgtaaaa actataaaag aatcaaaaac
     3481 tgtagtatgg aatgggccaa tgggagtatt tgaatttgaa aactttgcaa atggtacatt
     3541 atcagtagct agagctatgg cagagttgac tgatgcaact actgtaattg gcggtggaga
     3601 tagtgcagca gcagttaatc aattaggatt tggtgacaag atgactcacg tatcaacagg
     3661 tggaggagca tcactagaat ttttagaagg taaagaatta ccaggtattg cagcattaga
     3721 taataaataa actaatatat agaggagatt gtaaaatgag aaaacctata attgcaggaa
     3781 actggaaaat gcataaaaca ataaaagaag ctttagaatt tgtaaatgaa gttaaagata
     3841 aagtaaattc tgataaagta gaagctgtta tatgtgcacc atttacttta ttaaaggatt
     3901 taaaagaagc tactaagggt acaaatataa agataggtgc tcaaaatatg cactttgaag
     3961 aaaaaggagc atttacagga gaagtttcac ctcttatgtt aaaagaaata gatatggact
     4021 atgttgtaat aggacattca gaaagaagac aatacttcaa tgaaactgat gaaactgtaa
     4081 acaagaaagt tttaaaagca ttagaagtag gaatagaccc aatattatgt gttggagaaa
     4141 ctttagaaca aagagaagct ggaaaaacta aagatgtttg taaagttcaa gttgaaaaag
     4201 ctttagaaaa tgttcttaaa gatgatttat caaaagtagt tgttgcatat gagccaatct
     4261 gggctattgg aactggtaaa actgcaactg ctgaagatgc taatgacgtt atatcttata
     4321 taagagaagt tataaaagga ttatatggag aattagctaa tgaagttaga atacaatacg
     4381 gtggaagtgt taaaccttca aatgtagccg aaataatggg tcaaagtgat atagatggag
     4441 ctttagtagg tggagctagt ttagctagta atgattacct agaccttgtt aatttctaag
     4501 gagtgaaatg atgaaaaaac cagttgcttt aattattatg gacgggtttg gatacaataa
     4561 ggatgttaag ggaaatgcaa ttgctgagtc caaaactcct aatttagata gaataaaaaa
     4621 agagtatcca aacacattga taaatgctag tggtcttgat gttggtctac ctgatggtca
     4681 aatgggaaat tcagaagttg gacacactaa cataggtgca ggtagaatag tatatcaaga
     4741 tttaactaga ataacaaaat ctataaaaga tggtgacttt tttgctaata aagttttatg
     4801 tgaagcaatg gataatgcaa aagaaaattc tcttcatgta atgggacttt tgtctgatgg
     4861 aggagtacat tcacatatag accatcttaa ggctataatc aaaatggcaa aagataaagg
     4921 cgtacaaaag gtgtatgttc acgcatttac tgatggtaga gatactgacc ctcaaagtgc
     4981 attagaatat gccaaagaag tacaagctag tatggatgaa attggagtag gtgaatttgc
     5041 tacagtttct ggtagatact acgctatgga tagagataag agatgggaaa gagtagaact
     5101 tgcatacaat gccatggtta gaggtattgg tgaaaaagca aattctattg aggaagctat
     5161 tcaaaactct tatgatgatg gaaaaaatga tgagtttata atgccaacag ttattatgaa
     5221 agatgataaa ccagttggaa gtataaagga aaatgattca attatattct tcaactttag
     5281 accagataga gctagacaaa taactagagc tttagtttgt gaagagtttg atggatttaa
     5341 aagagaagat attaaaaact tctttgtatg cttaacagag tacgatataa ctatagagaa
     5401 tgtgcatata gcatttggac ctcaatcttt agccaacact ttaggtgagt atctagctaa
     5461 aaatggaaaa actcaactta gagctgctga aactgaaaag tatgcacatg taactttctt
     5521 cttcaatggt ggagtagaag agccaaataa aggtgaagaa agactattga taccttctcc
     5581 aaaggttgca acttatgatt taaaaccaga aatgtcagca tatgaattga cagataaagc
     5641 tttagataaa ttaggagaag ataagtttga ctttatagta ttaaactttg caaatcctga
     5701 tatggtaggt cacacaggaa gtatagaagc ggctataaag gctgttgaaa ctgtagatac
     5761 ttgtgtagga aaattgatag ataaaatagt agagttaggt ggaagtgcaa taataactgc
     5821 tgaccacgga aatgctgaat atatgttaga cccggaaaca ggaaaaactg taacggctca
     5881 ctctattaat ccagtaccat ttattgtagt tggtcaagaa tatgaaagtg caaaattatt
     5941 ggatggagga agattatctg atatagcacc gactgtttta gatatgatga agttagaaaa
     6001 gccagaagaa atgactggtc attcacttat ttcaaaatag tcgattctga taagtagaga
     6061 ataatatata aactacaata taaatttaaa aataatgcag tttaggttta ttttgtaaag
     6121 tttagacttt atgacataag atttagattt tgtaagttct ggatattgta aggtgtagat
     6181 gaaggtatat tttataaaat ttacaagata gacaatttat aaaata
//
LOCUS       DAMJVX010000192         6160 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMJVX010000192 DAMJVX010000000
VERSION     DAMJVX010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6160
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.198"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            136..3315
                     /locus_tag="PC407_003220"
     CDS             136..3315
                     /locus_tag="PC407_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2498629.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKRDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNIHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKETTIENKLTSSQFTKENNHYVFKGTDYDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIYKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIIDLFN"
     gene            complement(3600..4364)
                     /locus_tag="PC407_003221"
     CDS             complement(3600..4364)
                     /locus_tag="PC407_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2498630.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4601..6094)
                     /locus_tag="PC407_003222"
     CDS             complement(4601..6094)
                     /locus_tag="PC407_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2498631.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTVSFAIPLCENINVDCNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINKGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKVNELKDIEKSRITHGKILFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
BASE COUNT         2306 a          844 c          716 g         2294 t
ORIGIN      
        1 atagtaaatt gttatttaca catatattgt aatagataaa agctattcat atctgcctta
       61 attttagtct taaatttaga gtgtaaattg aatgcattta gattataata tatacagtga
      121 ggtaaggtga ataatatgaa aatagatatg atatataaac taataagaag aaatacagaa
      181 ccatcaagat ggtcaagagg tgtaaaatat tatactaatc aattagttga ttatatagac
      241 atagatattg atgacctaca aaataacctg ttgacatttc gtggtggagt caattctgaa
      301 gataattata gcttttattt taattcaata actatagata taaagcactt taagataatt
      361 gaaaatgatt gtagttgcgc tgactgtcaa tcaaagggca aaagtatttt tatatgtaag
      421 catatagttg ctattgtgat aaaaagctta aaacaaataa aaaataaaag agatgttcct
      481 cctattctaa tagatatatt aggtgcagat aaggataagc tttctaatac aaattctaaa
      541 acaagcttag atttaagcaa gcaattactt tctttgttag acaatactaa aagagaaagg
      601 gttaatttag atgtattgat aaatgtggat tcaaataaag caattgctga atttaaaata
      661 ggaattgata aaatgtacat tttaaaaaac attactgaat ttgcatattc aaaacttaat
      721 ggatatgaat tagattttgg gaagaaattt acttacaacc ccaacattca ttattttgct
      781 gatgaagatg agaagttagt agatattata gaagaatatg ggacaaatct ttcatacaac
      841 ttttcttcat cacattcaaa aaaatttatg gagataaatg caagctcttt aaaacaattt
      901 ttaaatgcta ttaagtacaa ggattttacc cttatttttt ctaaagagga atatcatcca
      961 aagatattaa atgatactct acctgtaagg tttgatttta aaaatataga agataaaata
     1021 gtcttatatt ctaaagattc actacctgtt ccacttacta aaaaaaatga tgtactttta
     1081 tatgatggca atatctattt attatcacat aaaaaagctc atgattattc aaaaatatat
     1141 caaatattat caaagactaa agagttaaaa tttgataaag aggattctaa ggatgtttta
     1201 ggattgctag ttcctagatt aaaatctata tctcaagaag ttcatttaga tgataacata
     1261 aaaaataata taacaaaaga ttttaaatct gagttctact ttgatatgat tgatggcaac
     1321 atatgttgtg atgtaaagta tatatatgat gatgaggata aaaaatttgt gctccccaac
     1381 atacaaaagg aaactactat tgaaaataaa cttacttctt cacaatttac aaaggaaaat
     1441 aatcattatg tgtttaaagg aactgactat gacctattta catttttaga tttacaatta
     1501 gaatctctaa aggaatttgg agatatatat tattctgaga agtttaaatt gaaaaaaata
     1561 tataacgctt catcaattca agcttcaatc aatcagacta accagaatta cctagaattt
     1621 aattttaata tatctgatat aaatccaaaa gagtacaagg atattttgaa agcttttcaa
     1681 gaaaaacgta ctttttataa acttaaagat ggaaacttta tagatttatc agaaagagaa
     1741 acaaaagatt tttttgaatt agttgaaaat ctagatataa tggataaatc aaaaaataat
     1801 aaaatttata aaaataaagc tctatatata aatgatgtaa tacgtagtaa aaatttaaag
     1861 tttatagatg gtaaaaaaga actagacagt atatgtgaaa aatttagaaa ttttgatagc
     1921 ataaatttag aaattcccgc aaatttaaat gcaaaccttc gtgattatca aataaatggc
     1981 ctcaattggt ttaaagtttt agattattat aaatttggtg gtatattagc tgatgaaatg
     2041 ggtcttggta aaacaataca gactattgca tttttattat cattatctaa taaaaaaagt
     2101 cttattgtta caccgacatc actcatttat aattggaaaa atgaatttga aaagtttgct
     2161 ccagatataa aagttttatt gatacatggg aataaaagag atagagaaaa atgttttatg
     2221 gaattagaaa attttgatgt aatacttaca acatatggta ctcttagaaa tgatttagaa
     2281 aaatatagtg aaataaagtt tgattattgt atattagatg aagctcaaaa tataaaaaat
     2341 ccagtagctt tagtaactga gtctgtaaaa agtataaatg cagaaaataa atttgcactc
     2401 actggtacac ctatggaaaa taatctacta gaattatggt ctatatttga ttttataatg
     2461 cctggctatc tatatagtaa agcaaagttt caggaattgt ttataaataa agaggataat
     2521 gttaaaaacc tgaaaaaatt aataaaacct tttattctta gaagaagtaa aaaacaagtt
     2581 atgaaagaac ttccagataa gattgaaaaa aacttttttg ttgaattgaa taaagagcag
     2641 aaaaagatat atagtgtata ttcaaaagat atacaagata aaatgaaaga taaaaatcta
     2701 aagaaagata aaatagttat attttcttat ttaactaaac taagacaact ttgtttagac
     2761 ccaagtatag tagtaaaaga ttacaataaa aaaagttcaa aaattgaaac atgtttagaa
     2821 atacttaggg atagtataaa cgaaaaccac aaaatattat tgttttcaca atttacttct
     2881 gtgcttaaga acataagtaa ggaacttgat aagtataaaa ttaaatacca ttatatagat
     2941 ggtaaaacta atgccaaaga aagacttgaa cttgtagatg agttcaataa tagtatggat
     3001 aaaaaagttt ttttaatatc tttaaaagca ggcggaactg gtttaaatct tacatctgct
     3061 gacatggtaa ttcactttga cccatggtgg aatccatcag tagaaaatca agctagtgat
     3121 agagctcata gatttggtca aaaaaattca gtacaagtta taaaattgat tgctaaaggg
     3181 actatagaag aaaaaataat aaaattacaa gaaagtaaaa aagagcttat aaatcaattt
     3241 ataaatggag aactttctaa tgaaggtgta ttaaaaagtc tatctgatga agagataata
     3301 gacttattta actaaattat ttaatcctac agcttaacta tataaattta ttctaacatt
     3361 tatatagtta agctacaata tctgaataaa taaaatttca tattgttatt taatcccaac
     3421 tttacaatct aatgatatct ctttctatac atattgcata tacaaatgta ataacttgac
     3481 tattagaata ttttgtttta acaatcctat ttcatcataa taatgaagta tatgtacact
     3541 cacttttgta agttttgcaa tccattttac agttttcatt attgtaaatt ttggttaaat
     3601 tattcctcta aaaatttctt tgctacattt gaaaaatcta atccattcga taaaattgca
     3661 ggatgaccac ctttatcaaa caaatacatt ttaccatttt taatcttttc aagaagtaat
     3721 gaaaaagtat tttcaaaaaa atcactagat attagagata caaactcatc ttctctactt
     3781 cctgttaaaa gcacctctgg ttgcatagtt tctagaggct tatgaaaaaa ttttcctata
     3841 gttttataat gttcaaaaat agcttctgta tcattatcta caactttttc ccagttttct
     3901 ccttgattat aaatataaaa agcttttgca ccatcatctt gctttgatgc ctcacgctct
     3961 attttaacat tttgtacaaa tgactctaaa ggaacttctc cttcaaaact atctgcaatt
     4021 accttattca ctaaatcagg tctctcaagt gcaacattta atgcaacaag agctcctcca
     4081 ctactaccaa ttatgtttac ttttttatag ttcattgctt ctaaaaaaca tatcacttgc
     4141 atagcctcat caaaccataa gtcagcagaa aatttatcga ccatttgaga ttttccatga
     4201 cctacaaaat caattaatat tactttatat tcatctttat aaaaatcgat tatatcatta
     4261 aacattttgg aagaggctgt atttccatgt aaaaaaagta atggtgtacc atccccaatt
     4321 tcattatagt agcactgttt gtcgttatag ttaaaatatg ccatatatta cctcctttaa
     4381 tatccctata ggtatatatt tattacttat atttaaaatg actattgtca taaacattta
     4441 aaaatagtat agcttagaat gtagataaag tcaacattcc tttccttaac aacttctatt
     4501 gttttttgaa acttcatttc gataacagaa taattcataa atattgatac tcttacttaa
     4561 atatatgatt tcatcatcag agagagcagt aataactaaa ttatcgtata tctcttttca
     4621 ctttaaccat ttttttcttt ataggtatct ccttatattt ttttaatatc aaatctccct
     4681 tataatttaa tatatctaca tatgaacata aatcttgaac ttcaattaca cctatatcat
     4741 cattagttat accttttata ttactaaatg caccaactat atctaaaact cttattttct
     4801 tcttcttacc agcatttaaa tataatttaa caacttcact ttctatattt agttttatat
     4861 ctttactttt gtaagaattt ttatcaactt tttttctctt tgatactttt tttaggattc
     4921 cttttgaata ttcttcaaac aaaattttgc catgtgtaat tctacttttt tctatatctt
     4981 ttaattcatt tactttatat cctatatacg tttctatatt ctctatatat ttttcatcct
     5041 tttcagttac tatagtaatt gcctttccac tatttccttt tctcccagtt ctacctattc
     5101 tatgtatata gttttcttta tcttgtggca catcataatt tattactaaa gaaatatcat
     5161 caatatgtat tcctctggac gctacatcac tactaactaa tatattaaat ttttggtttt
     5221 taaaatcctt aattacaaaa attcttcttt cttgtgataa atctgcatgt aactctctta
     5281 ttaaaaaacc ctctttactc atcttattat ataatttaga tactttctct ttggtattac
     5341 aaaatattat cacactcttt ggattttcgc tgtatattaa ttcttttaaa aggatgtact
     5401 tctctcttcc atttgccttg attattttgt catctatttg tcttgttttt tgattagtat
     5461 cgctctcatt ttcttctata tttataacac tgtaatctag catatatttt tcacatatat
     5521 attttatctc ttcatctatt gtagctgaaa aaagacttac tattttctcc ttaggtaaat
     5581 ttaataatat tttttccatt tgttcaacaa aacctttatt aaacatttta tctgcctcat
     5641 ctattactaa atactttaca ttttccaact taatagaacc tttatttata tggtcaagta
     5701 ttctgcctgg agtagctaca acaatatgaa ccctttgttt caattcagct atttggtctt
     5761 ttatagattg tttgccaaat atagcactac atctcacctt ctttagtctc cctatatctg
     5821 atatctcatc ttttacttgc aaggctaatt ctcttgttgg aaccactata agagcttgaa
     5881 tattattgca atctacattt atattctcgc ataaaggtat tgcaaaactt actgtctttc
     5941 cacttcctgt ttttgaccta actactaagt tttgaccttt taataatttt ggaataactt
     6001 ctctttgtac tctagaaggt atattatatc ctaaactctt caatgactta agtatctttt
     6061 catttaactt aaatttttca aatgtatatt tcatatatta ccaccttatt atatttatct
     6121 tatctatata tttttctaca ttatattatt ataatacaag
//
LOCUS       DAMJVX010000193         6135 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMJVX010000193 DAMJVX010000000
VERSION     DAMJVX010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.199"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            106..756
                     /locus_tag="PC407_003223"
     CDS             106..756
                     /locus_tag="PC407_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDF2498632.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(896..2272)
                     /locus_tag="PC407_003224"
     CDS             complement(896..2272)
                     /locus_tag="PC407_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2498633.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2288..4201)
                     /locus_tag="PC407_003225"
     CDS             complement(2288..4201)
                     /locus_tag="PC407_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2498634.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4337..4969
                     /locus_tag="PC407_003226"
     CDS             4337..4969
                     /locus_tag="PC407_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDF2498635.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5108..5647
                     /locus_tag="PC407_003227"
     CDS             5108..5647
                     /locus_tag="PC407_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2498636.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAARKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(5823..5993)
                     /locus_tag="PC407_003228"
     CDS             complement(5823..5993)
                     /locus_tag="PC407_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2498637.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
BASE COUNT         2267 a          861 c          860 g         2147 t
ORIGIN      
        1 gtcaagttta atttagttgt aattgtatct tttattaaaa ataatataaa ttaaaacata
       61 taaataaaat attttataca atatattgta aaggaggtaa ctttgatgaa agcagaaaaa
      121 acaagtatat atccaattat taaaaataat ttaaacaaaa tatcaattgt tgaatataaa
      181 aagggtcaaa gatttgtagc atcagataaa gacttacaag aagtgttctt tataatagag
      241 ggagttgcat tagtggagtg tactactaga ggaggaaata aatttcttgt tgacattgta
      301 cctgaaaatg aatttatagg caaaatcagt tacatatatg aacataattt gaaatgtgat
      361 atatttgcaa agaccaatat aaaattattt cgatttgaaa aaaatgtatt tgaagagttt
      421 tatctcaaac cagattttat tgcactattt cacagaaaat gtacaagaag aatatatgaa
      481 ttatataaaa ccagaatggt aagagaacta ttttcttgca cagaagttat tgcctattgt
      541 atatcaaata aacaagaaaa taatgtctgc aatataaaag ctattcgtaa tttcagtgaa
      601 atctattcaa ttagtagaaa aagtagatac cattctctgc taaagttaac tgaaaaggag
      661 atagtaaaaa aaatgggtaa tacatatgaa attttaaact atgatgaatt acataaattg
      721 gcatttgaag taaaagagtt tttggaagat gagtaaaaat tgttacttaa tcaaaataaa
      781 gtataataat ttctgtaaaa aatagaaata gtctcactga aaatcaaaaa tactttagtt
      841 attttcaatg agactgttta gaaatattgt atacttataa tgtatagtat aattattaat
      901 caatttcctt tactatatta atagcccatt ttttagataa tgctctagga acaccaaata
      961 taaacttaac cacttcttca ctataagtga gtaaaacgag tacaaatata ggtgcacctt
     1021 taaatgcagc tataaaagtt agaggaatag aagctaacca catacatcct aattctagaa
     1081 atagagcata tttagtatct cctccacttc ttaaaatacc aattataacc aatgtattaa
     1141 aagatttaag agccattagt attcccatga taaagaatat cttagttata tcagaagcta
     1201 agctatcaga aacactaaac atttttaata ataatggtat atttaaaatt aaaataattc
     1261 caagtattaa accagctgaa aatacaagta tagaaaactt tttagcatat tcaatggctc
     1321 ttttaatatg gtcagctcca agttcatttc caagcataat agatgcacca gatgcaatac
     1381 atacagctgt cattatgaag aaattacctg tactggttgc tatctggcta gctgctatag
     1441 cagaagtacc agctcttgca tatgcaacag aatacaggac agttccaatt gcccatacag
     1501 agtcatttac aaatatagga aagctttttg atgaaaaaga ttttataaaa attttatcaa
     1561 tagcttttag attttttaga ccaaacttta atatatagtc ttttttataa aggtaaacat
     1621 aagtaatcat taaaattaat tcacatattc tagcaataac agtagctaga gcagcacctt
     1681 ttacacccaa tgcgggaaga ccgaagttac caaatataaa tccataattt aaaataacat
     1741 ttgttacaag tgcaaatgca ctacaaatca tacctaactt aggatttcta acacctcttg
     1801 aaccagtact aaatacagta cttacagcaa ttaatggata actaaatact gcaatactaa
     1861 agtaatctat acaaagtttt accacctcag aatcatacga aaatatgtgt ataattggct
     1921 ttggtgttag taaagcagga ataacaaata caaaacttaa aacaattgcc aatacacaag
     1981 taagacctgt gacttttctt atattactca catccttctt tccataaaac tgtgaaataa
     2041 aaactccagc acctcctgtg ataccagata aggacatatt aaaaagaaag aaaaattggt
     2101 ttgcaactcc aactgaagct acagatgtct cccccaaact acttatcata attgtatcta
     2161 ttacatttac agaagtagaa attaagttct gtatgataat aggtatacat aaagatagaa
     2221 gtattttata aaatctttta ttttcgtctt ttaaagaaaa tagttcttgc aaataatcct
     2281 ctccttttta cataaattct tcccactcat catatagaga ggcaaggtta ttttctaaat
     2341 taattttttc ttggctaact tctttagctt tatctggatt agagtatact tcttcttgac
     2401 ataaaagtat atctaatttt tcaatttcaa tctctaactt ttcaatttct ttttctatat
     2461 tttgtctttt tattctattt ttcttttcta tttctctttg ttctctctct tttctttttt
     2521 catcttttat ttgagttcta gttttttctt ctttttcttc tataatactc atctcttgga
     2581 tttgcctttt tttatctata taataatcat aatttcccag atattcagta attccatttt
     2641 catctaaaac taaaatttta tcaacaacag tatttagaaa gtatctatca tgtgatattg
     2701 taaatatagt cccatcataa ttagttaatg cctcttcaag gacttcctta gaatcaatat
     2761 ctaggtgatt agtaggctca tctaataaca ataagtttgc atttgatagt ataagtttaa
     2821 gtatggcaac tctagctctt tctccaccac tcaatgtaga aatcttctta aaaacttctt
     2881 catctacaaa taaaaatgcc cctaacatat ttctcaaagt agtttgagta agatgagtat
     2941 tattttccca aatctcatca attattgtgt tatctaaatt aagagttttt tgttcctgat
     3001 ggaagtagga aacatgtaca ttagttccaa atttaatatt gccactaaga ggttgtagtt
     3061 cattagttat tattttaaat aatgtagatt tgccaacacc atttgcacct ataagtgcaa
     3121 ctttttcccc tctatatata tctaaatcca agtctttaaa aagtattctt tctccataac
     3181 ccatagatac atctctaatt tgaagaacat cattaccact ctggacagaa ggcgtaaatt
     3241 gtatacgagc ttttttccta taagcttctg gtttatctaa aacgtctacc ttgtctaatg
     3301 ctttttcttt acttcttgca cgctttaggt gtttttctct accataagct tttagtcttt
     3361 caattgattc ttcttgcttt tttatttctt tttgttggtc ttcaaatttc ttaagctcta
     3421 gttctttttc tattttagat aactcaacaa atttagagta gttaccatta tatactttaa
     3481 gttttttgtt gtgaacttca aaaattctat ttacggattg gtctaagaaa tatctatcat
     3541 gagatattaa catgacagtt cctttatatt gttttaaaaa tacttctaac cattctattg
     3601 cttcagagtc taagtggtta gtaggttcat caagtaacaa tagagttggt ttcttaagaa
     3661 gtaattttcc taaaagaact ctagtttttt caccaccaga aagtatgctt attggcttat
     3721 ccatatcatt gtcactaaat cctaatcctt ttagaactcc tttggcttca gatttatatc
     3781 catatccatt taaatctgaa aataattcta gtttatgtga atattcatcc atgatttttt
     3841 ctataattgg agagtttgtt tttgtacttt cttcagatat tttaacctcc aattctctta
     3901 aataggattc catttctatc aaatttttaa aaacttctag cacttcttcc aaaatagtat
     3961 tatccgaata aaaattggta ttttgttcta aatatccgat ttcacaatct ttagaagtat
     4021 aaatatctcc actatcatat ccataaattc ctgaaattat tttaaataaa gtagtttttc
     4081 cagtaccatt aacaccgatt ataccaattt tatcgccttc atttactgta aaactaatat
     4141 tttctaatat agaatctata ccaaaacttt tatttaagtt attacatgac aaaacaatca
     4201 taaatttcta cctccttgaa ctatctaaaa ttataatgtt ttgatataat ataatcaagg
     4261 ttatatgtat tagttttgaa aaagaaatat tttattgtga atcaaacaaa aaagaggaca
     4321 cttttaggag gtgaagatgt tgggaaataa aaatatatca atggcagtta taagaaggct
     4381 cccaaaatat catagatatc ttggagactt attagatagg gatatacaaa gaatatcttc
     4441 taaagaattg agtgatataa tagggtttac cgcttctcaa ataagacaag atttaaacaa
     4501 ctttggtgga tttggacaac aaggatatgg ttataatgta gaagctcttc atactgagat
     4561 aggtaaaatt cttgggttgg atcgaccata caacgcagtt cttgtaggag caggtaactt
     4621 aggacaagct atagccaatt atgcaggatt tagaaaagct ggattcgaga taaaagcttt
     4681 atttgatgca aatcctagaa tgataggttt aaagataaga gagtttgaag tattagattc
     4741 agatacttta gaagacttta taaaaaacaa taatatagat attgctgtat tatgtatacc
     4801 taaaaatgga gcacaagaag ttattaatag agttgtaaaa gctggaatca aaggtgtatg
     4861 gaattttgca cctttagatt tagaagttcc gaaaggtgtt atagttgaaa atgtaaactt
     4921 aacagaaagt ttatttacct tatcgtattt aatgaaagaa ggaaagtaga caacttaaac
     4981 atattaataa tgagcagttg tgaagagtat tgagaaagtc agaagattta atattaaatc
     5041 ctctggcttt ttgaaatatt aggataaaaa tttaaataat gtatggaaat aaacaatatt
     5101 aaggagagtg gtttttgtgg aggaattatg gaaaaaggca attgaatttc atggtcatga
     5161 atgtccaggg ttagctatag gatttaaagc agcacttgca gcaagaaaat atttaggatg
     5221 taaacagtca ggagatgagg aaattgtatg tatttctgaa aatgatgctt gtggaataga
     5281 tggtatacaa gtcgtgctaa gttgcacaat gggtaaagga aatttactac ttaggatgac
     5341 tgggaaatca gtatatcatt tctttaatag ggaaaatgga aaatctataa gaatatatgt
     5401 aaagtcaaag ccatttcaaa tggatagaga aggatacaaa aagtacttat tagaaagtga
     5461 ttttgaagaa atatttgaga ttacagatac taagttaaga cttcctgaaa aagcaagaat
     5521 atttaaatct cttaaatgtg aagtgtgtgg tgaatttgca tctgagcaat atataaggat
     5581 tgaagaagga aagaaagtat gtcttgattg ctttaataaa tatgaaagat tttatgagga
     5641 gtattaaaat tataaattag attaaagtta aaagtattag atatgtacta acagtatttt
     5701 aagatgaatt aggtagcaac ataataaaat atataaataa tattacttga accatacata
     5761 attaaaaaga ggctgattag tatttagaaa ataaaatcag tctctttatg aataacgaat
     5821 tattattctg gttgaggtgc atctacagga caagctcctg cacaagaacc acagtctata
     5881 caagaaccag catctataac atatgcgtcg tctccagctg atatacagct aacagggcat
     5941 tcagcctcac aagcaccaca gcttatacaa gcgtcagtaa ttttatatgc cattttgaaa
     6001 tcctcccttt tatatgtatt gtaattcaat aacattataa caaatattat tgaatataac
     6061 aattaaatta attctacaac aaaaacttta ttatgatatt cttgttttaa agttttacaa
     6121 taaagtttaa tctgc
//
LOCUS       DAMJVX010000194         5980 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMJVX010000194 DAMJVX010000000
VERSION     DAMJVX010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.200"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            131..418
                     /locus_tag="PC407_003229"
     CDS             131..418
                     /locus_tag="PC407_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498638.1"
                     /translation="MKKILYALYSFIVIIANFRLKEKNYNFILLAIFFILLLIQNGVL
                     KTGYQNRATFYQDNYFNGEDKNDKIKFITIKIFIFLSLPLCFNILFNYLTS"
     gene            complement(956..1507)
                     /locus_tag="PC407_003230"
     CDS             complement(956..1507)
                     /locus_tag="PC407_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2498639.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1698..2660
                     /locus_tag="PC407_003231"
     CDS             1698..2660
                     /locus_tag="PC407_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDF2498640.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            2955..4799
                     /locus_tag="PC407_003232"
     CDS             2955..4799
                     /locus_tag="PC407_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2498641.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKRKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASNAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDIVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESIFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            5121..5705
                     /locus_tag="PC407_003233"
     CDS             5121..5705
                     /locus_tag="PC407_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2498642.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKDKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNVKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         2337 a          591 c          917 g         2135 t
ORIGIN      
        1 tgtcctttaa aaataaaaat taaaaaatat ttaatgctac tacaatagga actagaactg
       61 cacttaataa atatacagaa attagatttg gctcaatata aaatacaaat atagaattta
      121 ggtgtttttt atgaaaaaaa ttttatatgc tttatatagt ttcattgtta ttatagctaa
      181 ttttagatta aaagaaaaaa attataactt tattttatta gcaatctttt ttatactgct
      241 gttaattcaa aatggagttt taaaaacagg ttatcaaaat agagctacat tttatcaaga
      301 taattatttt aatggagagg ataaaaatga taagataaaa tttataacta taaagatttt
      361 tatatttttg tcattaccat tatgttttaa tatactattc aattatttaa cttcttaaat
      421 tatatttaga tataggagtg ttatgataaa agtaagatta tgtgttgttt atattaaata
      481 taaagtgcat atagtgataa aacaaaagtt tcatataaat gaagaaaaaa tataaaataa
      541 tttatttaac tacttaaaaa taaactaaaa gggataataa aatagctttt aggttatggt
      601 aataaaggta tttaaaaaga gtgcataagc actccaattg tgagaaatac atacacaaat
      661 cattattaca acaatgaaaa atgtacatat aagagacgtt ttgtatttgt tttaagtgat
      721 aatattttaa tactaactaa ataattatca caataataaa aaagaaaaag gagtattttg
      781 tactccattt gtcaaaaata taaaactttt atataagctt attataacat aattgataaa
      841 agtgtaaagc tatataatga atttataaag aaaaataaaa taaaaaagat aaatcctaat
      901 caaaggattt atctttttgt atagaaaata attattctat tattaaatgg catggctaaa
      961 ctggataata atttattatt actgctttca atgttttttc tccttgatta tggtatgtat
     1021 gaattgattc agctgaaaaa caaatagagt cattttctct aagttgataa atagagtcat
     1081 ttacttctaa ttttaattga ccttctataa tcataatata ttcctgtgat ttttcagaat
     1141 gaccaacaga tgtataacta tgaccttctt ctaattccat ttgaaataat tcgaagtttc
     1201 tagttggtgt attgggataa taacaataaa gacgataatc tttattttct gaaacttgta
     1261 catctatatc agtttttgat acaataaaag tttcattttg aggttgttcc agtattgcag
     1321 tatatggaac atttagtcca cttgcaattt tccaaattgt gtttactgtt gggtttgagt
     1381 ctcccttttc aatttgcgaa agcattactt tacttacgcc agataattca gcaagttgac
     1441 ctagacttaa gttacgttca ttacgtaatc tatttaaatt tttagcaata acagtattaa
     1501 tactcatagt aactccttaa aatgtaaatt attgacaaat ataatttacg aatgtataat
     1561 ttatttaaca ttatttataa tttacaaatg tataatataa tgaacgttaa atacactgta
     1621 catccgtaat ataaattata ctgcataaag agttgttttt caacggatga tagttgagag
     1681 attaaggggg aattgtaatg cgtgtactta ataatgttac agaggctata ggcaatactc
     1741 atatgttaca tttatccaaa atagcagatg aatttggagt tcaaggtaat atttatgcaa
     1801 agatggaata cttaaatccg ggatttagta aaaaggaccg tgtagcatta caaatgatta
     1861 ccgaagcaga aaatgcaggt ttattagttc caggtcaagc agttgtggaa ttaacaagtg
     1921 gtaatactgg aacaggacta gcaatagctt gtgcatgtaa aggatataaa tttatagcgt
     1981 gtatgtcaaa aggaaattct atggaaagag caagaatgat gaaagcttta ggtgctgaag
     2041 tagttcttgt agaccaaatg cctaattcag ttcacggaca ggtatctggt gaagatttgg
     2101 cacttgtaga aattgaggct caaaaaatag caaaagaaag aaatgcattt agagcagacc
     2161 aatttaattt agaagcttgt aatcatgcac atgaaaagta tacagcagaa gaaatgtggg
     2221 aacaattgga tggaaatatt gatgtatttg tagattttct tggaagtggt agtacttttg
     2281 caggatgttc aaaaacactt aaaaaacata attcttctat caaatgttat gttgttgaac
     2341 ctgaaacagc agcaatttat tcaggtaaag aaataactga ccaagggcat aaaatacaag
     2401 gtggaggata ttctatggat ttgccattag tagacaagaa acttattgat ggttatattc
     2461 aagttacaga cgaggaggca actgatgtag caagaaaatt agctaaatta gaaggtgtat
     2521 ttgcaggatt ttcttcaggg gcaaatgtgt gtgcagcaat taagttgtta aagagcagtg
     2581 aaaaaggaaa aaatatagtt ttaactctta atgatagtgg tttaaaatat ttaagtactg
     2641 atttatatga agaattgtaa taatagcaca atagttatgc aatgtttttt aaattaagta
     2701 tatagattta aattataaaa agtatctcaa tgtgattttt gaaagttata aaagagatac
     2761 ttttttattt tgtaaatatg gagtatccta cttataagta ttaagctata tagatattga
     2821 tgattagcta tgcaataaaa aagagtatgt gtttttgcat ttatacatca taacttggaa
     2881 taatacatat taatgttcta aatgtattta aaacatatga taaaattaga acaaacagat
     2941 acaaggagat aaacatgcaa aagagaattt taagaaattt tatatttacg ggtaaaaata
     3001 ttttaagtaa acttgttttt ataataaaat ataattttag tacattaatt ttatttgaat
     3061 tatttcataa aggattagcc ttatttttta tatggccatc tattagatat atatttgata
     3121 ttttaataaa aagtttacat atagtctacc taaatatgga taattttata aaagttttga
     3181 ccaatcccat gtcaatagtt ctgataatac tatcagttat aatactagct ttttatgcat
     3241 tttttgaatt tacctctgta attatttgtt ttaataaatc gataaaatat gaaaaaatag
     3301 ggttatttga actctttaaa atatcattta agaactctat aaaattgtta tatcctaaaa
     3361 atatactatt atttatattt gtgatattaa taattccatt agtaaataca gttttaattt
     3421 cagggtttat aggaaaaata caaataccag agtacatatt agattatata aagtctgata
     3481 taattttaaa tataatctat atgatttttc ttttaataat ctatatttcg gttattagat
     3541 ggatttttag cattcatgag ataactttaa atacagaaaa ttttaaaaga gcaagaaaag
     3601 aaagtgttaa tcttataaag agaaagatta taagaacaat tatttattca ttaatattat
     3661 ctttagtagt agctattatt ggatatgcga tttatcatag tggaattata tttataggca
     3721 tatggactaa atattgtact gatatggaat ttttgaagaa tgtatttata aatagatgta
     3781 ttgcatttga agaatatttt agattgattt tatcaatatt catttttata ttggacattg
     3841 gatttatctc agcaacttat tatcaatata aaggagtaaa tatttctaaa aataatatta
     3901 aaaaagagaa aaaatctata tcaaaaattg tttttaattt agtattaata ttattgattt
     3961 cttatataga gtcagttgca ttttcttcac atataaatgg catgtttaat acgtcatttt
     4021 cattttatac aacagcaatt gctcatagag caggagctga aatggctcca gagaatactt
     4081 tggcagcaat agaagaagct tcaaatgcta aagctgatta tgcagaaata gatgttcaag
     4141 aaactaaaga tggagaactt attgtacttc atgattcaaa ctttaagaga actacaggag
     4201 tagataaaaa tgtttgggat gtaaattata atgaagtaaa gacatatgat gcgggaagct
     4261 tttattatta tggaaaaggt tattatgatg aaaagtttgt tggggaaaag ataccaacac
     4321 tcaaagaaat gataaaatat tctaaaggta aggtgaaatt attaatagaa ataaaactta
     4381 atggacatga aaagggagac atagtaaaaa aagttgttca gttaattaaa gaaaataatt
     4441 ttgaaaatca atgtatagtt gcatctatgg ataaaactat attacaaaaa gtaaaaaaac
     4501 tggatgagaa tttactgaca tgctatttta tggcaattgc atatggtgat ttttcaaagt
     4561 taaattatgt tgatatatat ggaattgaat caatttttgt taataaaaca gtagtagaaa
     4621 agattcataa aatgaacaaa aaaatcttcg tttggactgt taatagtgac agtttaattg
     4681 agaaaatgct tgacctaaat gtagatagta ttattacaga taatccatac cttatagaga
     4741 gtgcaattta ttggaagaaa aatggattta ttagtcaagt gtcagattat ttattttaaa
     4801 tattctataa ttgtattatc cagtaaaaga gatattatta aaatcaataa tatctctttt
     4861 taacataaat aaggttgtta aattaaggtt attcttataa aaattgtgct tatcagctat
     4921 aaacaaggaa taaaagtgaa aaaattgctc tgagattatt acagttaaaa gagttttaag
     4981 atgacagaga gatagattgt caactgtata tttaataaat cttgttattt attaaatttt
     5041 atataagtaa aatatataaa ttaataaatc ttatgctaga atatatatga aaaacaatat
     5101 aaatttggag gattaaaaac atgacagagc gagaaaaaat gttggcagga gaactttacg
     5161 attgtggtga tattgagttg ttaactcaat ggcataaagc aaaagaccta gtaagagatt
     5221 ataaccaaac agattcctcg gatttggaaa aaaaagaaaa aatattaaat gaattacttg
     5281 gtggtaagga taaaaactta tggattactg caccgtatta tgtagattat ggaaataata
     5341 tttattttgg aaataattgt gaagtcaata tgaattgtac gtttttagat gataataaaa
     5401 ttatcattgg ggataatgca ctaatcgcac caaacgtaca aatatatact gcatttcatc
     5461 caactaatgc tggtgataga tttggtaatg tcaaagaaga tggttcattt gaattttgta
     5521 aaactcaaac agctccagtt actattggag ataatgtttg gattggcggg ggtgctatta
     5581 tcatgccagg tgttactatt ggagataatg tagttatagg tgcaggaagt attgttacaa
     5641 aagatattcc aagtaatatg attgcatacg gaaatccatg ccgtattatt cgagaaaaca
     5701 aataaagtta aattttcttg caaaatctca aaaaacacct ttcttacttt atacataggg
     5761 tattttccaa tactttcaca tccatattca tttgttaaat ttttgtggaa tgtagttttt
     5821 atcacttcta tcttacattt ccaccatttt ttataaaagt cattccttta tagatttatt
     5881 tataatagta tatattctat ttgatgtttg aataagcact tatttttaag taaaatacta
     5941 acttttaggt tagtattttg ctatagtatg atgattaagt
//
LOCUS       DAMJVX010000195         5975 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMJVX010000195 DAMJVX010000000
VERSION     DAMJVX010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.201"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(431..1147)
                     /locus_tag="PC407_003234"
     CDS             complement(431..1147)
                     /locus_tag="PC407_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2498643.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSREIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(1688..2488)
                     /locus_tag="PC407_003235"
     CDS             complement(1688..2488)
                     /locus_tag="PC407_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2498644.1"
                     /translation="MTFKNKKFTINKYEILSIILVLCLILTGFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNVGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(2803..3534)
                     /locus_tag="PC407_003236"
     CDS             complement(2803..3534)
                     /locus_tag="PC407_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498645.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(3521..4216)
                     /locus_tag="PC407_003237"
     CDS             complement(3521..4216)
                     /locus_tag="PC407_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2498646.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(4218..5009)
                     /locus_tag="PC407_003238"
     CDS             complement(4218..5009)
                     /locus_tag="PC407_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2498647.1"
                     /translation="MKNKFFTTLLTGFFAIVFFFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(5019..5831)
                     /gene="modA"
                     /locus_tag="PC407_003239"
     CDS             complement(5019..5831)
                     /gene="modA"
                     /locus_tag="PC407_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2498648.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
BASE COUNT         2083 a          842 c          727 g         2323 t
ORIGIN      
        1 agatataatt ataaaattgt agattattaa attagtgttg taaaacattt tattacttgt
       61 aaatgcatga aaccttaagg ttaatagtat caaagttaat ataagtgtaa gttctatttt
      121 taccttagaa aaaaggctta tgacattctc taagaatttg actttagtct gtttattttt
      181 tttatacaat gtaaaacata gtatagtcaa taaaaaactt gataatataa tacctatgca
      241 gacaaactct aattctactt ttgacaaatg tacaggagta ccgaaaaaat aaaaataagg
      301 gagagcaaac tctttataat aacaattaat attataagaa aagttattat tcaaatcata
      361 aactgcttca aataatatag aaactaatag aaacagtaat aataataaaa taaattttgc
      421 tttagaatca ttaataatta agtttttcaa ttttgtaacc aatgccccac accaccttta
      481 aatattttgg ttcttttgga ttaatttcaa ttttttctct aattcttcta atatgaacag
      541 caatagttct ctcacaacta taagcatctt cattccagac attttcataa atttcacggg
      601 aagagaaaat tttacctttt cgtgacataa gaagtgtaag tattttatat tcaagtggtg
      661 tcattttaat ttcctcacca tctaaagaaa ctatttttgt atctgtattt agctctaggc
      721 caccactttt tagaatataa ttagtatcat ttgagttatt tgtattacta aaatcaccta
      781 aatttatata ccttctaagt tgagatttta ctcgagcagt aagttctagt ggattaaatg
      841 gtttagttat atagtcatca gcacccatat taagacctat aattttgtcc atatcttctg
      901 attttgcaga taagagaatt attggtatat tccttttttc tcttatttta agtgtagttt
      961 taagcccatc taaattgggc atcattacat ccattattat taacttaata tcattttcga
     1021 ttagtatgtc caatgcttca agtccatcat atgcctttaa aacattaaag ttatcaggtc
     1081 tcaaatagaa ctctatagca tcaactatct ctctatcatc atctacaacc aatatatttt
     1141 gattcaattt attaaccccc ttgaaatttt atttttaacc aatttttatt ttataccaaa
     1201 aagaggtatt agaaaacagt aagcccgttc attatgaaat aaaaactagt acttttaata
     1261 tattgtcaat gcatatagta aatttaaaag tatagacgcc catattaatc tgaaggtaaa
     1321 agtttatgaa aatatctagg gtaatctatc acactcctta tgttattttg ggtttattaa
     1381 ttttttaggt ttaaacatat attaacaaat ataaataaca aatgagtttc ataattatta
     1441 agaatttata acaaatattt attttaatgt agtttaatat aaataggtag tttaatgtag
     1501 atatgtataa agtatttaat aaggcttata ggttttaatg atgatatctt tatatatttt
     1561 aagagatgat gaaattagat atgggtaata gttgaatgag aaaaagtagt atagcacaaa
     1621 aagtgctata tcacaaaatc tcaaataatt tccttcttat aacttaaatt aaaagaataa
     1681 atatttctta taaattaggt gtatgaagta tagcaggaac atctttatta tttaataact
     1741 tatcaaatga tttatggtca gacttatagc caataccttc tctatattct aattcatcaa
     1801 caattttgta ttgttgacag gcatttttaa ccttttcata gtcttcagta gaatgagctt
     1861 tataaactat cttattatta aaagttattt tatcagcaaa tttaggtttt acaaaatatt
     1921 tagcatcttt tattttacca acattatata aattttggat aacagaacca ttttttaaat
     1981 gttgtaaatg gtcatctaac ttataagtat gtgctatatg agttacattt ttaaattttt
     2041 cttcaaatgt aagagcaatt ttttccatgt aattgaagtc agtattacca tttgtatttg
     2101 tttctggtaa taatattata ttttccctta ttacaccaag gctattaaga gaagctaaaa
     2161 attctctatt tctatattct gatttttgta tatttgttag gttttcatat tttttatatt
     2221 tatcaaagac ggatatacca catccagtag atactaaaac tacaaataca tgattttttc
     2281 ctctttcttt tatagcatta acaatagcac tcccagccca caacacttca tcatcttgat
     2341 gctgaacata gaagacaaca taatctttaa attttttatg actataattc acatagctac
     2401 taaaaccggt taaaatcaag cataaaacta gaataataga tagaatttca tatttattta
     2461 tagtaaattt tttattctta aatgtcattt aatcatctcc tgaaaagttg tcttagttat
     2521 attatcaaac aaagtactaa aaattatttc ttaaatgctg aaaaatatag agtagattaa
     2581 tggaataaaa ttttttttat tagtttaaaa taaagaaaac atttatgtat aagactaatc
     2641 aaaatattaa taattgatag gaattgagat ttaaagggat tgtacattta ttcgggattc
     2701 attttaatag aattttactt tagtacttag aataaaagta ataacttttt aaatttaaag
     2761 agtacacata tatatttgtg tactccttga tgatatgatt gcttatttac tacaaaaaca
     2821 gagtcttttt aaacctaaaa tatttgatgc accagctatc gtagttccat aatagtaaac
     2881 tggtttgtta agtgatagaa aatttataat tgagttatta cataatgtac ttcctgtgca
     2941 aatcactaaa tcagcccaag atattacatc ttcataatct cgtataccat cttcaatctt
     3001 aacattatat tgaatagtat ccacaaattc aggatttaaa tctaatactc ttgtttcgaa
     3061 aaagtcctta atattatcaa ttatggctgg ttgatatcct ataattgcta cttttggatt
     3121 accaaattcc atttttataa actcagggaa tttttttgca catacctctg gctcgttatt
     3181 tttacaatga atggttctat ccgtcttgcc taagtatttc ataactgcat ttaatgatgc
     3241 tataaaaaga cttctatcat gaggattgtc atgtaaatct aatgataata tgtcttttaa
     3301 agaaccttca aatgtagatg gagtagaagt aaatgcttgt cctttagctc ctttaaagtc
     3361 agcttcaagc attatttctt taccaactat aattgggaaa tctttacgct tagtatttcc
     3421 aattgcttct tttgaagata gagatttgga cacaatactt atcatatcca tatttaaatt
     3481 atatttatcg acttgtacta atagataatc tctcaaaata tcaaatatat tcatagaaaa
     3541 aagattctcc tttatttttt atttcttcat ttaaatcaaa aatatgattt atttttccat
     3601 tgttcattat tcctatctta tgagcaaaaa acattgcttc agaaatatca tgagtaacat
     3661 gaatcactgt tggtttgtat tttatgtaga catccttcaa aattttgctt atatgttttt
     3721 ttgtaatata atccaatgaa ctcataggtt catctaaaag taatatatca ggttcagcta
     3781 ttaaggcacg tgcaaaagca actcgttgct tttcgcctcc acttaaattt ttaggatacc
     3841 tattttttat atcttctatc tgcattaatt tagacaagtg gttagcctta agaatctgtt
     3901 catctatagg tatttttctt aatttaagtg gaaaccttat attatcttca acattcatat
     3961 ttggaaataa ttcaaaattt tgatatacaa atcctatatt tctattttct ggaggtaagt
     4021 ttgatattaa attttctttg tatgatacat ttcctttagc agatggatat cttcctgcta
     4081 atgtttctag gaatagagtt ttacctgacc cactagctcc taataaaaca aagtattcat
     4141 gattattaat atttaaacta ttttcttgta atttaaaatt aggtaaatca agagataaat
     4201 tttttatttt taacatatta attcttatca acctctctat taaaaaatat ctcaaaaagc
     4261 atcaagcatg taagtgagat aaatatcaaa attacagcta ttccgattgc aatgtctata
     4321 tcaccagtag acatatttaa atatatagat gtaggaataa tttcagtttt catacgtgta
     4381 gaacctgcta tcatagctac tgcaccaaat tcaccaagtg ctcttgacca agtcattaca
     4441 gttgcagata tgattccagt ttttagattt ggaagaatta taaatctaaa tgcttcccaa
     4501 ctattacatc caagggtacg tgcaacaaat atcatctttt cattactttc atttagtgaa
     4561 gtgtgaagta ttttaatagt aagtggaaga tttacaaata cttgtgctaa aactatacct
     4621 tgctttgtaa atataaaatc aagcttaaaa atcttataaa tggaatctcc aatccccatt
     4681 cttccaaaca aaagtaaaag agcaattcct gatactaagt gtggaagaga catagggaga
     4741 tttattatct gtataatcag ttttttaaat ggtaatttag taatatgtaa aaaatatgct
     4801 actggaatag ctaataaaag gcatactata gtagatatta aagatgtttt aatactaagt
     4861 gttattgcat actgtacttc cacggatttt aagcatatag gaacataact tatcccttta
     4921 atgattaaca taagaagtgg tgatacaata aaaaaaaaga ctatagcaaa aaatccagta
     4981 agtagtgtag taaaaaattt atttttcaag tttaatcact acttaacagg ttcaaaacca
     5041 tattttttaa atatctgttt agcttcatct gactctatat aatttataaa tttattagct
     5101 aaatcttttt tatcactata gtttacgatt cctgctgata tgagttggtc tatattttta
     5161 tcttgggcaa tttctataac ttctacatct tcattgccaa agaccgaatc ttttgtggct
     5221 attgtagcat cagctttact tgatgttaac tgtacaatca tttcatttac agttgcagct
     5281 gtagagacaa cattatcatt tatagatttt aaattatttt tttcaagtat tttttgtgta
     5341 gtcttaccta tagcattaga ctctttgtct cccaaaataa gtttaactcc atctttattt
     5401 aagtcttcca atttttgaat attagctgga tttcctttag gtacaactat tgcaggtgta
     5461 tgatgtgcaa ccgttttatg ttcacctaca agttcttttt caaaggcctt tgaataaatt
     5521 ggttcagaac caacaataaa tacgtctcct ttgtgacttg tctcaatttg agctattagc
     5581 tgtgcagaac ctgcatagct atattcaact ttgacattat tttctttctc aaattttttt
     5641 gctatttcat ccattggttt ctttaaacct gcccctgaat agacaaaaag agtctctttt
     5701 tcttttgaat catcagctgt tgtcacttta gtattagatt catcattagc tttgttttct
     5761 ttagaattac atgcagtaat acttaatgaa ataatcatag ctaaagctag tactgaaagt
     5821 tttttaaaca taagatattc ccccttagta aattagtgta aattcttttt tagtatatca
     5881 tactaagaaa acgatgtcta agtttttgtt atgttttata atgttttgtt atgttttatt
     5941 atgtatagtt aagaaaaaat ctatttataa tgtca
//
LOCUS       DAMJVX010000196         5941 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMJVX010000196 DAMJVX010000000
VERSION     DAMJVX010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.202"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            105..671
                     /locus_tag="PC407_003240"
     CDS             105..671
                     /locus_tag="PC407_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2498649.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            711..1292
                     /locus_tag="PC407_003241"
     CDS             711..1292
                     /locus_tag="PC407_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2498650.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(1349..2266)
                     /locus_tag="PC407_003242"
     CDS             complement(1349..2266)
                     /locus_tag="PC407_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2498651.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            2396..4027
                     /locus_tag="PC407_003243"
     CDS             2396..4027
                     /locus_tag="PC407_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2498652.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            4030..4977
                     /locus_tag="PC407_003244"
     CDS             4030..4977
                     /locus_tag="PC407_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDF2498653.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALATEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5262..5525)
                     /locus_tag="PC407_003245"
     CDS             complement(5262..5525)
                     /locus_tag="PC407_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HDF2498654.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(5624..5869)
                     /locus_tag="PC407_003246"
     CDS             complement(5624..5869)
                     /locus_tag="PC407_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498655.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
BASE COUNT         2135 a          817 c          985 g         2004 t
ORIGIN      
        1 ataaatatag ccaacttagc ctaaatacgt atataatata gctaatatgg ttcttatact
       61 tgataccatc aataaaacta aaaatataaa aaggagaact taaaatgagt gttagtttac
      121 aaaaaggaca aagagttagc ttagtaaaag ataataatcc tgtaaaaaat ctagtagtag
      181 gcttaggatg ggatatgaat aaattgggta aaaaaaatta tgacctagat gcatttgcaa
      241 tagcattgac taaccaagat aaaatgaatg ttagaggtga cttggtttat tttggaaatc
      301 ttaaacaccc ttctaaagct attatacaca ctggagataa cttaactgga aaaggtgaag
      361 gtgatgatga acaaataatt gttaatttag aagacatacc tgaatatgta cataaaatag
      421 tttttggagt ttctatatac aaagctaaaa aaagagacca agattttggt caaatgaaca
      481 actcttttat aagattaatt gattcaaatt ccaagcaaga attatttaaa tataatcttc
      541 aagaagactt ttctggtaaa gtaacagttt tggcaggaga aatttatcgt agaaatgaag
      601 aatggaaatt taatgcttta ggaattggtc aaaatgaaga gcttcgtgaa ttaattaata
      661 catataagta ataattacaa taaaaaattt ataatgagga gttggtttaa atgggtataa
      721 ctttagcaaa aggacaaaaa gtaagtttaa caaaaagtaa ccctggtcta aaaaaagtaa
      781 tagtaggact aggatgggat attaataaat atgatggtgg atttgatttc gatttagatg
      841 cctctgcatt tttaactgga actgatggaa aagtaacgaa tgatggagac tttatattct
      901 ataataattt aaaacacgct tctggagctg ttgaatatat gggtgataat agaacaggtg
      961 ttggtgatgg tgatgatgaa caaataaatg tagacttatc taaaatacct caaaatattg
     1021 ctaaaatatc atttagtgtt actataaatg aagcaattac aagaagacaa aatttcggac
     1081 aagttgaaaa ttcatatata agaatatata atgaagaaac taacgaggaa cttattaaat
     1141 atgaattggg tgaagatttt agcatagaaa cagcaatagt cgtggcagaa ctatatagac
     1201 ataatggaga gtggaaattt aatgctttag gttctggatt tgaggatgga cttgcaggat
     1261 tgtgtaaaaa ttttggcgtt aatataggct aatatatcta aataaaaata agagtatatt
     1321 gatatactct tatttttatt tagatatatt ataaactgtt tacacttcta ctcttctttc
     1381 tcgcttgttt tttcccatat tctactgaaa tatcaaaaaa tgctgaaata ttttctgtaa
     1441 aaaacttgct tctatgatat gcaatatcga atgttctatg taagttaata ccttcaatat
     1501 cacaagccca caaaagatgc ttttttaaat aatcctcaat aagtaattcg gatataactg
     1561 caatgccaaa attatctaca actgctctta atatagcttc agagttataa catgaccatc
     1621 taatattcat tgggattttt aattctttta attgcttttc taattgtgct cttgtaccac
     1681 ttcctaattc acgcataatt aatggttgat ttgaaagttc tgaaactttt atagagtctc
     1741 ttttgtagaa ctcatgcttg tgtgaacata taagtacaag attatcatta ataatgcttt
     1801 ttgtaacaat atctgggtgc ttaatttttc cttctacaat cgcaatatct aattcactat
     1861 taagtaattt ttttgcaatc agtcttgtat cttctacaaa tacttcaggt tctataagag
     1921 gatttacctt gtataattct aacattatcg ggcttattac acatgtacca acagttactg
     1981 ttgccccaac tcttatacgg atattttttg cacaatgctt catacaatca ttcatttctt
     2041 catttacagc taaaagatgc ctcccatatc caagaagttt tttccctgcc tcagttaaat
     2101 ataatttttt tgaaattcta tcaaataact taacaccata ctctttttct atttctgaaa
     2161 tagcttggct aactgatgac tgggtaatat acatatttct tgctacttca ctcattttac
     2221 cacattcagc aaccttaaca aagatttcaa gactacgaat tgtcatatta tcacaacctt
     2281 agcaaacatt agtaaatgct tatgatttta ttaatatttt agtattttac aaatatattg
     2341 tcaagaagta taattttatt atagagttat aaagtagatt ggagtgtgat atattgtgaa
     2401 agtattaatt ataggtggag ttgctgctgg aacaaaaact gctgcaaaaa taaaacgtga
     2461 aatgggtgat aattgctctg ttacaatact aaataaaggt gatgatattt cttatgcagg
     2521 ttgtggtctt ccatattacg ttggtaagat aattgaagat aaatcatctt taatcgtaaa
     2581 tacacctgaa agtttttcta aactaacagg agcagaagtt cgttgtggag ttgaagttac
     2641 gtctattgat agaaatgcaa aacaagtaac tttgaaaacg ctttcaacag gagaaaaaga
     2701 aaatctaagc tatgacaaac ttgtaattgc tacaggggca gacccaataa aaccaccaat
     2761 acctggaata gatttacctg gagtattctt tatgcgtact ccaaatgatg ctataaaact
     2821 aagagattct attgaaaacg gtattaagag agctgttgtt attggtggag gtttcattgg
     2881 tcttgaaatt gctgaaaatc tttctgcaat gggtatacgt gtaagtgttg ttgatatggc
     2941 tgaacatgtt atgcctggat ttgatactga ttttgctgaa tacgttgaaa atcatatggc
     3001 tgataaaggc ataatgattt ttacaggtga ccaggttgta ggtattgaag gtgaaaataa
     3061 agttgaaaaa cttcgtacta aaaatagagc tattaaaaca gatttagtgg ttatgtcagt
     3121 tggaatccgt cctaacacag gttttctaaa agacacaggt ttagaatttg cccctaataa
     3181 tacaattatt gcagatgaat atatgcttac aaatgataaa gatatatacg tagttggaga
     3241 ctgtgctttt gtaaaaaatt ctttaacaaa taaacctaca tggtcaccta tggggtcatc
     3301 tgcaaatcat gagggtagaa tttgcgcaca aaatatatct ggaaaatcta aaacttacaa
     3361 tggtgttctt ggaacgacag tagtaaaatt acctgagctc aatgcaggaa aaacaggtct
     3421 aagtaaagaa atagctgaaa aagaaggtta taatgtttgc tctgtaacca tagctaccga
     3481 tgataaagct cattattatc caggtgcatc taattttatt ataagaatgg ttgctgaaaa
     3541 aaatactaga aaattattag gtgttcaagt tatgggacct ggagcggttg ataaaatagt
     3601 agatatcgct gctactgcaa taactttagg cgctgacctt tattcattag aaagtatgga
     3661 ccttgcatat gcaccacctt tttcaacagc tattcaccct tttgtagtgg cagtaaatgt
     3721 tttacaaaat aaacttaacg gagaacttga tagtgtttta cttgacgaaa ttgaggattt
     3781 tgacagttgg actaaacttg atgtttcaaa agctccttct attcctagtt tacgttatat
     3841 tcctgttggt gaaattaatg gagaaattga aggtcttgct aaggatgaaa aaattcttct
     3901 tatatgtgct aaaggtaaaa atagctatat ggcgcaaaat cgcttaagac gctttggcta
     3961 tacaaatacg aaagttcttg aaggtggaat actattttat ccaaatctta aggtaatgga
     4021 ggagtaaata tgagtaatgt tacagcagaa caaaaaaagg aagtaaaagg tagaggtttt
     4081 ctaccaagca gggatggaga acattttgca gcacgtataa taactgtaaa tggtgtaata
     4141 aatgcatcac aagcaaaaaa aattgcagaa gctgctgaaa aattcggaaa cggtcagtta
     4201 gcatttacta ctagactaac agtagaatta cctggtgtaa aatttgaaga tattgaagct
     4261 ctaagtgaat ttattgctag cgaaaatcta ataacaggtg gaactggatc aagggttcgt
     4321 cctgttgtgg cttgtaaagg tactgtttgt gttcatggtc tagctgatac acaggcactt
     4381 gctactgaaa tacacgaaga attttacaaa aattggtatg atgtaaaact acctcataag
     4441 ttcaaaattg gtgttggtgg atgccctaat aactgtatta aacctgacct aaacgatctt
     4501 ggaattgttg gtcaacgtgt acctgactat gattcagagc tttgtgttgg ttgtaaaaaa
     4561 tgtgctgttg tagaggtctg tcctgttaaa gcagctaaat taacagataa aggtaaactt
     4621 gaaatagaca gtaatctatg taataactgt ggtaagtgta tagaaagttg taattttgat
     4681 agtattgaag aaaaagaatc tggttataaa gtctatatag gagggaaatg gggaaaatca
     4741 gttcgtcctg gaacacagat tgatagatta ttttcaaaag aggaacttat gactttaatt
     4801 gaaaaagcaa ttcttttatt tagagaacag ggtaacacag gtgaacgttt tggaataact
     4861 atcgatagaa ttggtgctga taaatttata gaaatgcttt tatcagatga agttttggaa
     4921 agaaaagaag agatactaga ggctctttta caccttacag gtggagctgt atgctaacaa
     4981 taaatatttt aattgtacag ataacctatg gaaaaaattt tagttaagaa taattaaaat
     5041 tctatatatg atatatgata aatgaggcta tctcaaaaca cagatacgcc tctttttcat
     5101 tgttgaaatg ataaagtata tttgagttta tacaaaaaag agtgtctcgt gaattataaa
     5161 attcattttg aggcacccac attattttat aataattgct tattaatttt attttaacta
     5221 tataaaaagt acaaaaagta agaatatcca atattctaaa attaattttg aattgtattg
     5281 attgaaaagt ttactattcc actatttcca tcttgattta ctactagata ccatgttcca
     5341 gtattaggaa ctctcattct acatggattt ttctttgcaa atccaccata gtaatcataa
     5401 tttctttgat ttttaaattt cacataattt atatggtcta acaatattac attggcttgt
     5461 ttctctaaat ttacctctac aatatcccct ttttttaagt tcatcttact ataactatat
     5521 tccattgaat tactcctcca gtatcgaata atctttgtta tttactcatc tcaaatatgt
     5581 aaaaattcat ttaaaatatt acaaatacta tataattaga ctattatcca gttttaacct
     5641 tatttactac aatccaaagg ttattaaaaa tattattggc aacaacttct ccatcccata
     5701 caactctttc ttctttattt tctaataaag catagtaccc agagtcagaa ctttcatctt
     5761 tatagactgt aacataaaac tcttcatcac tatattcagg tatattttta aagtcttcag
     5821 atattttaca tgttaatata tacccttcta attcttcatc tataaacaaa gcaattcctc
     5881 caatctctat atttttacta attataccat attatgtcat aaacttttta tttcattaca
     5941 a
//
LOCUS       DAMJVX010000197         5880 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMJVX010000197 DAMJVX010000000
VERSION     DAMJVX010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.203"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            90..710
                     /locus_tag="PC407_003247"
     CDS             90..710
                     /locus_tag="PC407_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF2498656.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(876..1412)
                     /locus_tag="PC407_003248"
     CDS             complement(876..1412)
                     /locus_tag="PC407_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2498657.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(1525..2199)
                     /locus_tag="PC407_003249"
     CDS             complement(1525..2199)
                     /locus_tag="PC407_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF2498658.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            2403..4277
                     /gene="asnB"
                     /locus_tag="PC407_003250"
     CDS             2403..4277
                     /gene="asnB"
                     /locus_tag="PC407_003250"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2498659.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEYVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(4369..5805)
                     /locus_tag="PC407_003251"
     CDS             complement(4369..5805)
                     /locus_tag="PC407_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2498660.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVHFTRY
                     IGVDDSIDKYIEKVKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWM
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIAQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
BASE COUNT         2259 a          683 c          861 g         2077 t
ORIGIN      
        1 ttaatattag attcattttt tgagattggt atataataaa catatagagt atataataat
       61 agttaaagtt aaaaaagggg aaaagcaata tgcagtatat acaagaatta cttgatttga
      121 ctcaaaatta ttgggtgtta gccacaataa tcggcttatt aagtgcattt atagaaagct
      181 ttataccagc cttaccactt gttgctatag ttacagcaaa tgctgcaata caaggattat
      241 ttattggatg tttgttatca tggattggct caggacttgg gacaacttca ttattcttat
      301 taataagcag atttaatgat agtaagcttt ttaataagtt gagaaatagt aaaactgaaa
      361 aagcaataag ttggatggat aaacagggat ttaagttatt atttatagcg tatgcctgtc
      421 catttatgcc tggttgttta gttactatcg catcagcttt tggtaaaaaa gatttaaaag
      481 attttgtgcc agcaatgttg gcaggaaaat ttgtaatgtt tatagttata agttacgtgg
      541 caagtgatat agaaggtttt ataacaagtc ctttaaagat agcatcattt atattattgg
      601 tgtttttgtc atggaagatt ggaaatagag taaataaaaa tcttgaaaat cataattatg
      661 atttccatca taaaaaacat cacaatgata aagatgataa aatgatttag cttgtacgaa
      721 ataaaacgta tcttaaaatg atttagtact aaattatttt aagatacgtt ttattttttg
      781 tattaaggta caaaattttt agttcttttg taaactaaaa tatataaaac ttatttatac
      841 ttagttcaaa atattgttat ttattttttc tacaactata ataaaatata aaattgatat
      901 ccaatcaaaa aaataactat gaaatttaat agcttagata tgaatttttc tgtttttgag
      961 ttagttttta taggtagtaa agaaatgttt atggtaatgg gataaaacaa ttcaatacct
     1021 tctttagtaa taaggtcaag acacaagtga gatagatatc ctattataaa cccactggac
     1081 aaacatgaga aaactatatt attgttagtg aagattgtca gagatttaaa aaaactataa
     1141 ataagtaata aggcaattag actgtgagta aaccctctat gtctaaacct acttccaaat
     1201 aatttgtata ttaacataaa ttttttactt atgtaagaac ctctcatatc tatatctgga
     1261 aataaagaac ctaaatatgc aaaataaata taaattataa ttaatactat tttatatgca
     1321 atatcgtatt taactaggta cagattgttt ataaagggga gtatcaaaag tgcgaatata
     1381 taacctcccc tttcatgtgt ttcttttatc atataaaaat cctcctgaat cgtcactata
     1441 aacattatat tggtaaattt agactatctt ttattatatt atatagcaaa cataagtttt
     1501 tttcaaaagg attttaatat ttaattaatc ttgagatttt aaatttttgt aattttctaa
     1561 agcaccattc caatttacta cattaaacca gttgtcaatg tattcatttc ttctattttg
     1621 atattttaaa tagtaagcat gctcccaaac atcaagtcct attataggag ttaggttttt
     1681 acttacaggg ctatcctgat ttggagtagt cataatagat aatttcccat ctttagtagc
     1741 tacaagccaa gcccaaccag aaccaaagac atctaaagca gatttttgga actcttgctt
     1801 aaatttttca aaagaaccaa agtctctatc aatagcttct tttaaagatt cagaaggtat
     1861 ggttttttct ggcgtcatta tatcaaaaaa gaatttatga ttataagctc cacctgcatt
     1921 atttcttaca gttgtagcaa tatctttagg taaagaatct aaattttgca ataattcaca
     1981 taaagaataa ttataaagct caggatattt ttcaagagca gcatttaatt tatcaacata
     2041 agcttgataa tgcttatcat gatgcagttt cattgtttct ttatctatat aaggttcaag
     2101 tgcatcatat gcataaggta atggtttaac cttaaatttg ttattttcag gtgtaaaagc
     2161 aaatgaagtt atgcactgtg agattataaa taaagacata ataactggta ttaatatttt
     2221 tttcttcata aaaatcacct cctaatatag tatttctaga aataaaaaaa aatatgaaat
     2281 attaacagat aatttataag aatttaatag caatttcatt ttaaattgaa taagttttaa
     2341 agccaaagac aaaactatga aatatagaat aagactaaag gacagaaagg aattttgatt
     2401 atatggacgg caatatttca atttattata aaggtgatat atctaaaaaa gaagatatta
     2461 aatttaaaat aaaagaatct gtaaataata caagactttc tagtagaata gagtataagg
     2521 atgaggaaaa aacttttgat tttgaaagat ttaatgtaat tgatgctatt tatggaaata
     2581 aaccatttga aaaagatgga aaagttattg tttttaatgg aaatatatat aactatcatg
     2641 aaataaaaga agaattaatt aaaaaaggac acaattttac tacaaatgat gatattgaag
     2701 tcttattggc atcttatata gaatttggta agaattgtgt acataagatt aaaggaatgt
     2761 tcaactttat aatctatgat agagaaaatg aaagtatatt tggagcaaga gatttatttg
     2821 ggataaaacc actttattat ataaataagg aaaatgcaat aattttttcc tcagagtata
     2881 agtttttact tgaatatatg aaaaatttaa atataaatga aagaagctta caaagttatt
     2941 tttcatttca atatgtactt cctgaagata ctatgataca aggaataagg ctgataccag
     3001 caggtcacta ttttagggta gaaaatggta tactatcttt aaagagatat aataaacttg
     3061 agtttagaag tagtacaaaa tttttttata ctaaaaatca cttaggcaat agagatgtga
     3121 atgaggatga tataagaaat atagtagtag attcaatagt aactcatatg gaagaggaga
     3181 aagaaattgg aacatttttg tctggtggaa tagattcatc tattataact actgtggctt
     3241 cacaaattaa tccaaatata aaatcttttt ctgcaggctt tagtgtcaaa ggatatagtg
     3301 aattagaagt agcaaagaaa acagctgata agttaggtat agaaaatatc cagattaata
     3361 taacacaaga tgaatatata aaatcattac cgaatgtaat ttattctttg gatgacccta
     3421 tagcagaccc ttcagaagta ggtctttatt tcttaattaa agaggcaggt aaacatgtta
     3481 aagtagcatt gtctggagaa ggagcagatg aattatttgg aggatataat atatataaag
     3541 agtatagtac tatgaaatca gttgtcaatt cacctactta tattaaaagt atattaggaa
     3601 gagtttcaga acttatgcca aacataaaag gaagaaatta tctttataga gctacaacac
     3661 cattagaaaa aagatatata ggaaatgcaa aagtttttga aaacagcgaa gtaaaaagat
     3721 tcttttttaa gtataaggaa aagaatatat atgaatacct gttgtctaat ttgtatagag
     3781 atgcacaaaa aaataattac gattatataa gtaaaatgca acatatagat gtgaatacat
     3841 ggcttcaagg cgatatactt caaaaaatta gcaaactttc tacagctgag caggttgaac
     3901 ttagagtacc atttttatat aaagatgtat ttgatgtagc aaaaaattta agaatggagc
     3961 aaaaaataaa taagaataat acaaaagttt tacttagaga ggcgtttaga gaaatagtcc
     4021 cagaatatgt tgttcaaagg aaaaaattag gatttccaac tccaataaga gtttggttaa
     4081 aagattctct aggggatatt gtaaaggaaa ctatttataa ttcaaatgta gatgaattta
     4141 tagataaaaa atatgcaata aaacttttag atacgcatct taaaggtcat agagataatt
     4201 cgagaaaaat atggactata tttacatttt gtctatggca tcaattattt attgagcata
     4261 aaaatgtcga gtattaatac ttttttcaat attaatcaga gggcaaaagc atgcaatgtt
     4321 ttcaccctct tgattattat ggaataggat aagtagaatt ttgattgatt agtttactat
     4381 ctcaataaaa ttattggcaa atattttgac aagattactt tcaacagcta aatttaagtt
     4441 ttgtccatca tcatatccag cggaaatcaa tcctagtaag tttccaaaca tatctaaaag
     4501 agctcctcct gaactaccac tagagattgg agctgtaaat tgtatcattt gaaccgtttc
     4561 aaaatcacga aaagctgaga taattccatc tgaaactgta ttaaaaagac ctaaagggct
     4621 tccaattgca actatttttt gacctctcac aggtttttta ctgactttaa caggtattgg
     4681 tttgcatttt ctatcaacct ttatcacagc taaatcataa tctgaatggt attttataat
     4741 ttggtaagat gtataaactt tatcatcatt ttcaaatcta actaaaaatg aatacccatc
     4801 attgactaca tgaagattcg taagtatata accttcatta tttattacta ctccagaacc
     4861 actcttaaac ggtttatttt cgttattgca cacaacaatc ataacaacag aagctgataa
     4921 ctttgcaagg tcttcataag aaagttctac ttcctcatct tgtgcaattt ttttagatgt
     4981 tagatttgtg ttattaagtg tatttttaga gttaggttta ttcgtagtag gagaagtctt
     5041 attggtattt tcactgtaat ttatatttgt aatatgcctt tcaaaaggta tttctaatat
     5101 atttggagtt ttcctagaaa gttcaaatag agtagaaaat tttgaacatt caggatataa
     5161 atcaagaata tctttatttg gatttatata taaaatatca cacccaagtt gtgataaaaa
     5221 atataagaaa aaaagttctt gccttttaat atttccaaag tatactatct ttggtgaaat
     5281 agtagtcttc accttgttat aaaataattt aggtagaaaa tagtccatcc aacacaaaag
     5341 tttaactgca aagtttttga tgatagagga tgtacaattt ggcatacttg tggaaaataa
     5401 atctaaagta tttttaaatg caatcttttt agtccaattt aaagtgtcat tttctatttt
     5461 acatggaaag tcgatattat ataaatccca actgttaaac ttagaactat cataaatatt
     5521 aaaaatttta agcatcttat ctgtttgttc tttgtcaaaa tttattttta gattgtttaa
     5581 tctaatatag ggaattgtag gattattaaa aaaataagta tctatatttt taactttttc
     5641 tatgtattta tcaattgaat catcaacacc tatatatctt gtaaagtgga cttctttaat
     5701 atatgaagaa ctcttatttt tataggtagt atcagatata aattcaatta gaggttcagt
     5761 tgactttttt attgaaatct ttaatggttt aatagtacaa atcatatttt ttctcctata
     5821 ataagatgat ataatctatt ataaaacaac ttaaaacaat atagtacaat aattttgtag
//
LOCUS       DAMJVX010000198         5871 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMJVX010000198 DAMJVX010000000
VERSION     DAMJVX010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.204"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            129..1493
                     /locus_tag="PC407_003252"
     CDS             129..1493
                     /locus_tag="PC407_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDF2498661.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSNFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVENTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            1632..2798
                     /locus_tag="PC407_003253"
     CDS             1632..2798
                     /locus_tag="PC407_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2498662.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2915..3697
                     /locus_tag="PC407_003254"
     CDS             2915..3697
                     /locus_tag="PC407_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDF2498663.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYAESLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3816..4469)
                     /locus_tag="PC407_003255"
     CDS             complement(3816..4469)
                     /locus_tag="PC407_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2498664.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4493..5857)
                     /locus_tag="PC407_003256"
     CDS             complement(4493..5857)
                     /locus_tag="PC407_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2498665.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2201 a          748 c          900 g         2022 t
ORIGIN      
        1 tttttttatt ggaagattta tgttatattg agatgaattt attatgatat aaaaaataat
       61 gttaggataa atttaaatct ttttctaaaa atgtattaat ataattctta aagaaaggat
      121 ttttaacaat ggatataaaa gagttagtta aaatgcaaag aaaatattat aatacaggta
      181 aaacaagaga tatatcgttt cgtatagaac agttgaagaa acttaagtta gttgtatcac
      241 aaaatgaaga aaaaatactt ttagcactaa agaaagattt aaataaatcg aattttgaag
      301 gttttatgac agaggtaggc atgttttata gtgaattaaa ctttgctata aagaatataa
      361 gaaaatggag caaaataaaa agagttaaat caagtatggt taattttcca tctatatcca
      421 aagttgttcc acaaccttat ggtgttactc tcataatgtc accttggaat tatccctttc
      481 aattagcact tataccatta gtttggtcat tagcagcagg gaattgtgtt atattaaaac
      541 cttctgaata ttctgtgtca acatctagtg tagttaaaga catagttgaa aatacttttt
      601 caaaagaata tgttgcagtt gttcaaggaa gtcaagaaga aagtgaaaag ttactattgg
      661 aaagatttga ttatatattt tttacaggaa gtactaatgt tggtaaaata atcatgaaat
      721 cagcatcaga gcatttaact cctattactt tagaacttgg aggaaaaagt ccttgtatca
      781 ttttaaaaga tgctaatata gaccttactg ccaaaagact tacttgggga aaactaataa
      841 atgcaggaca aacctgcgtg gctccagatt atgttttagt gcatgaagat agaaaaaatg
      901 agttaataga gaaaataaaa tattatacta ataagtattt tggagataat ccatgtaata
      961 atgagcaatt tcctaaaatc ataaatcaga gacattttaa tcggatatta tctcttattg
     1021 ataaagataa gatagtctat ggtggaaatt ataataagga gacattaaag atagagccaa
     1081 ctatagtaga caatgttaat tgggatgata atataatgaa ggaagaaata tttgggccta
     1141 tatttcccat tttaacatat aaagatttag atgaagtaat acaaaaaatt atacaaatgc
     1201 caaatccatt ggcactttat atttttacaa aaaataagta cctagaaaat aaacttttag
     1261 aaatgattcc agcaggcgga tgttgtatca atgatacagt tactcatata gctacgaatt
     1321 acttaccttt tggtggtatt ggtgaaagtg gtatgggctc atatcatgga aaagctggat
     1381 tcgatacatt tactcactat aagagtgtgt taaaaaaatt aaatttggat gtccctatta
     1441 gatatgctcc atacgataat aaattaatta aggttcttaa aaagataatg taggttatat
     1501 tgtagaataa taaatataaa taaaattgag tatttgtatt ctgaatatat tttattttta
     1561 atttggtata tttgttataa aagtggtgat tttgtgataa aatttaatta tatagaaata
     1621 aggaggaaaa aatgaaaatt aattggaata caaaatacac aacaatagca atttatacat
     1681 ttataatagc tgctagtagt attatatttt atctagtatc atcacaaatt gatgtatttt
     1741 ctaataattt agatgctata tttacaacat tacaaccttt tataatagga tttgctattg
     1801 catatcttct taattttatt ttaaagtttt atgaagacag aatttttata aaaagtgaga
     1861 aattaaaaaa actaaaacaa agttcaaaaa gaggcttggg tcttttatta acatatgcaa
     1921 cagctacatt gatactttac ttatttatgc atttcgtatt gccacaagta attgagagta
     1981 ttgttggact agcaaatgat atacctatgt atgtaaacaa tgcaactaag cttattgata
     2041 aattaatgac tgatttaaat cttgatgagc aatactttaa tttggcagtt gataagtgga
     2101 atgagtttgt tacatatatt ataaagtttg taacagattt aatcccaata ttaggaagta
     2161 tgttaaagaa tgtagcctct agtatatgga atattgtact aggattaatt gtttcagtat
     2221 acttattaat tgataaagaa aaattctatg gattaagcaa aaaaataact tatgcagtat
     2281 ttacagaaaa acaagctgcc agaattttag aattaactca tagaagtaac tatacatttg
     2341 gaagattttt aggtggcaaa atattagatt cattcattat aggtattcta acatttgtaa
     2401 tacttacatt agttaaaatg ccatatacat tattaatatc agttataatt ggtattacaa
     2461 atattatacc tttctttgga cctttatttg gagctatacc atctactcta atagtgatgt
     2521 ttgtatctcc tataaaagct ttctggttat tgttgattat attaataata caacagatag
     2581 atggaaatat tataggacct aaaatacttg gagattcaat tggtatatct gcattttgga
     2641 ttttgttctc cttattggtt gcaggaaaat tattaggttt tattggaatg gttataggag
     2701 ttccaatgtt tgcagtaatt tattctataa ttaaggatat tgttgaaagt aaattggaca
     2761 aaaagggatt accgacagat acttccgatt acatgtagct aaaaaatatt atgtataatt
     2821 aaaaatatat aaattaagca atatttaaag cactgagaaa gttaaactct tggtgctttt
     2881 tagatataaa attattatag aagaggtgtt ttttgtggca tttatagaat gtaaatttaa
     2941 atctaaatat actggaggag aaactgatat aattgtaatt ttgcctgtag caaatggaaa
     3001 agaacttttt agtggtaaag agatatatga ttttgaaggt aaatttaaaa cattatactt
     3061 atttcatggt ttagaagatg accaaagctc atggattaga tatactaatg tagagcatta
     3121 tgctaaaaat aaaaatatag ccctagtaat accaagagta gaaactagtt tttatacaga
     3181 tatggcgtat ggacaccaat attttacttt tgtaagcgag gaattaccta aatttgttag
     3241 agctatattt cctttatcag ataaaaggga agataacttt gtagctggta tgtctatggg
     3301 gggatatgga gctttaaaat tagcactttc aaaaccaaaa gagttttcgg cagttgcaag
     3361 tttttctggt tcgcctgata tgattcatga gttagaaaat caacaaatag aaaccaatgc
     3421 agacatttta tttcacgatt ttggtagacc agaagacgct agaaatagtg agaatgattt
     3481 actttacata cttaagaacc ttaaagaaag aaatgaagat attcctaaaa tttatcaatt
     3541 ttgtggtgat agagattttt tatataaaaa taatcagatg tttaaaaaat atgcagagtc
     3601 tctgggtgta gatgttgaat atgaagagtg tgtagggggg catgaatggc gtctttggaa
     3661 tttatggata aagaaattta tagatataat tgggtagtac acttatggta ttaacaggta
     3721 tactataaaa tatctttaaa tatgataaag actgaaatcc taaaatcaaa ttatgatttt
     3781 aaaatttcag tctataagaa tttaataaaa ttttattaag ttcttacacg agttattatt
     3841 ggagtaaaat tgtcaactat atctatatag tatttactca attcctctaa aatctcttcg
     3901 cataaatccg tactatgacc aagattaata agaaactgtt ttgtaaaatg ctttattaca
     3961 gcttgagaag atatatcgta cttgtgtgaa gtataaactc taattatttc aggtttcata
     4021 gcaagtatac caatacaaga aagtattaat tcatcatgtt ctatagattc tttatcatta
     4081 acatatcttt tcatgaccat ttcggttaca cttctttgat agcttaaaat agcttcttca
     4141 taagctatat ccaaataaaa atttctatat gcttcgtttt ttagaaggaa acaaaggtca
     4201 gcattcatag caagaaagaa catcatcata gtaccttctt catggtagtt atagattaaa
     4261 gactgcattt catttcttat atcttcatat attatagctc ctatttcgct tttacttgag
     4321 aagtaatagc ttattagacc taaatttata ttagctcttt ctgcaatttg cctagttgtt
     4381 gtcttatgat aaccttgctc aaaaaataat tctctagcag cacttataat actagctttt
     4441 gtttctttgc ccttttctga ttttgacata tttactgtgt atacctctct tttcaatctt
     4501 tttttgaaaa tttgctaact ataaatattg taatagaacc acctattata ggaaccaaaa
     4561 ttctgtcaaa tacagtaaga ataccaatca tatttaataa tgccaatata acacttacta
     4621 taacagcata gatagctcct ttttttgtag cgcctttcca gcaaagacca attacaaatg
     4681 gtgcaaatat tacacctgta cataatggag ccggccaaaa caataattct aataaactat
     4741 caaatttaag tgctatgatt gcacctatta tagctgtcaa agctgatgca atttgagaat
     4801 actttagagt attttttaaa tcattaaatt ctatattagg atgtaataat ttgttataaa
     4861 tatctcttga tataatagca ctcattgagg tcattaatga atcggcagta gacattatgg
     4921 cagccaatat agcagctaag aataatacag atattaaagg tggcaaagta gtcgatatca
     4981 tttgaggtaa aacctgaaca gatgatatgt ttggaaataa actaccacca atagctccaa
     5041 ttattcctat agggaaggca agaaataata gtataaatcc tgcaattaca ctacttttaa
     5101 ctgcattttt agcattatca gcacattgat atcttaaaaa actttcataa gaaaatattg
     5161 agcctaaaac taatggtaaa actgtataca taaaatcact aatcgattta tttcctggaa
     5221 agaatggttc ttttaccatt gattgtaaca taagtaaagg gttgtcgcca actaatttta
     5281 agcttatata agctactatt ggcaaaaaca ctatacaaat agcaagttga ataaatgctg
     5341 atactgcaac tccccacatt cctgatgtat aagtatatat aattataact ataactccta
     5401 taattacagc tatatcataa ggaataccaa atgcagtaga tacagaacca aaagaaatta
     5461 attgactacc cataatagct agtatagata ttggtgttac aagactttga taagctctta
     5521 aaaatacact atgaccatat ctagtttcaa tataatcagc aggtgttata tcacaagata
     5581 agcttcttaa ttttcctgcg aaaaaggcat ttataaagca agctaaagct gcacctatag
     5641 tagaatacat tccaacccaa agtcctcttt cagctcccca ttgaactcca ccaactaagc
     5701 accctcctcc aaaagatgta gcagcaatgg ttgcagctag tataggcaaa ggaagatttc
     5761 tacctgcgat taggaaatcg gatgctgttt taactttttt tgaaaaataa aaaccaatcc
     5821 atatgtatat tgcacacgta acaattaaaa gtaacatagt cttttctcct t
//
LOCUS       DAMJVX010000199         5782 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMJVX010000199 DAMJVX010000000
VERSION     DAMJVX010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.205"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            49..2280
                     /gene="pflB"
                     /locus_tag="PC407_003257"
     CDS             49..2280
                     /gene="pflB"
                     /locus_tag="PC407_003257"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDF2498666.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFS
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            2440..3174
                     /gene="pflA"
                     /locus_tag="PC407_003258"
     CDS             2440..3174
                     /gene="pflA"
                     /locus_tag="PC407_003258"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDF2498667.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3296..4966)
                     /locus_tag="PC407_003259"
     CDS             complement(3296..4966)
                     /locus_tag="PC407_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HDF2498668.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
BASE COUNT         2039 a          830 c          833 g         2080 t
ORIGIN      
        1 aattttaata gtattattta tcatttaaat ctaaggagga aatacttaat gaatgcatgg
       61 caaggattta aaacaggaag atggactaaa gaaataaatg ttagagagtt tatacaatta
      121 aactattctc catatgaggg aaatgactct ttcttagctg gtgccactga aaatactaaa
      181 aaattatggg acgaagcaat ggtcctattt aaaaaagaaa gagaaaatgg tggtacttta
      241 gatgttgata caaaaactgt ttctggaata gctgcatatg ctccaggata tttagataaa
      301 gaattagaaa caatagttgg gttacaaacg gatgcccctt taaaaagagc tgtcatgcca
      361 tatggtggta taaagatggt tgaaaattca tgcgaagctt ttggatatga attagaccca
      421 gaaataaaag atatatttac taaatacaga aaaacacaca accaaggagt ttttgatgtt
      481 tatactccag aaatgagagc tgctagaaaa tctggtataa taacaggtct tccagatgct
      541 tatggtagag gtagaataat tggtgactac agaagagttg ctctatacgg tgttgatgct
      601 ttaatagaag ataaaaatga gcaaaagaaa tcattagaag tttcttgcat ggatgaggaa
      661 gttatcagat taagagaaga aataactgag caaatatcag ctttaaatga acttaaaaaa
      721 atggctgaat cttatggatt tgatatatca aaaccagcaa ctaactcaaa agaagctgta
      781 caatggttat actttggtta tttaggtgct gttaaagacc aaaatggtgc tgcaatgtct
      841 ttaggtagaa catctacttt cttagatata tactttgaaa gagatttaaa agctggaatc
      901 gtaacagaag aagaattaca agagtatatg gaccattttg ttatgaaatt aagaatggtt
      961 aagttcctaa gaactcctga ttacaataac ctattctctg gagacccaac ttgggtaact
     1021 gagtgtatag gaggtatggg aatagatggt agaacattag ttactaaaaa ctcatttaga
     1081 atgttaaata ctctatatac attaggacct tctccagagc caaacttaac agttctatgg
     1141 tcaactaaat tacctcaagg attcaaagat ttctgttcta aagtatctat agatacaagt
     1201 tcagtacagt atgaaaatga tgatttaatg agagcatact ggggagatga ctatggtata
     1261 gcttgttgtg tatctgcaat gagaattggt aaacaaatgc agttctttgg agctagagtt
     1321 aacttagcta aaactttatt atacgctata aatggtggtg tagatgagaa atctggtgtt
     1381 caagttggac caagatttga acctataact tctgagtact tagattatga tgaagttatg
     1441 agtaaatttg aaccattcac tgattggtta gcaacattat atgtaaatac tttaaatgta
     1501 atacattata tgcatgataa atattcttat gaagcattag aaatggcatt acatgataga
     1561 gatatattta gaactatggc ttgtggtatg gctgggttat cagtttgtgc agactcatta
     1621 tctgctataa aacatgctaa ggttaaaact attagaaatg aacaaggaat agctgttgat
     1681 tttgaaatag aaggagatta tccaaaatac ggaaacaatg atgatagagt tgatagtata
     1741 gctgtagagt tagttgaaag ctttatgaat aaaataagaa aaaataaaac ttatagaaat
     1801 tcttatccaa ctcaatctat acttacaata acttcaaatg ttgtttatgg taagaaaact
     1861 ggtaatacac cagatggtag aagagctggt gctccatttg ctcctggtgc taacccaatg
     1921 catggtagag atactaatgg tgctttagct tcattatctt cagttgctaa attaccatat
     1981 gaacatgctc aagatggtat atctaatact ttctctatag tacctgctgc tttaggaaaa
     2041 gatatgactg aaagaataaa caatctttct gcaatgatgg atggatactt ctctcaaaat
     2101 gctcatcact taaatgtaaa tgtatttgat agagctactt tagaagatgc tatggaacat
     2161 ccagaagaat atccacaatt aactataaga gtatctggat atgcagttaa cttcataaaa
     2221 ttaactaaag agcaacaatt agatgttata aacagaacat tccacggtaa aatggcttag
     2281 tctttaaatt tttatacttt aactaagatt actactgtgt aaaaaataaa aataaaaggc
     2341 tatcttatat ttgaattatt tctggtataa gatggccttt tttagacaaa tattataact
     2401 attgataagg ttattaaaat atattgtgag gtgaactcta tgattaaggg aaagatacat
     2461 tctatagaaa cgtttggtac tgtagatgga ccaggaataa gatatatatt attttttcag
     2521 gggtgtcctc ttagatgcaa atactgtcat aacagggata catgggatat taaatcagga
     2581 aaagaataca cagttgatga aataataacg gatgctctta agtatacttc ttttatgaag
     2641 ttttctggtg gaggtataac tgcctctggt ggtgaatcca ctcttcaacc agaattttta
     2701 agtgaattat ttaaaaaagc aaaagaaaat gatatacaca cttgcttgga tacatctgga
     2761 ttcgtagata tagaaactat agacccagtt ttagataata ctgatttggt tttacttgat
     2821 ttaaaacata tggttgagga aaaatctatt gatttgactg gtgttggtat ggataaggca
     2881 ttaaaacttg ctagacactt agaatcaaga aatattcctg tatggataag acatgtttta
     2941 gttcctggca ttactgatga tatagataat ttagaaaaac taggtcaatt tgtagctaca
     3001 ttgaaaaatg tagaaagatt tgaattgcta ccataccatt ctatgggaat acacaaatgg
     3061 gaaagtttag gtatcgatta tgaactaaaa gatgttcctg atgccactaa ggaagatgtc
     3121 caaaaagcta gtgaaataat ctctaaattt ggagtgaaag tatataatag ttagtgtagt
     3181 atattaatcc tttatagaag acataacaaa atagggtaaa gtgtaaattt ttaataaact
     3241 tcaaaacttc cactttgccc tatttttata tactatatat ctcattttat gtacatcact
     3301 ttgatagttg aaatttataa aattcagagg cacttacagg tcttccaaat aaaaaaccct
     3361 gaataatatc tacacctaag ctttttacta tatcatactc ttcataagac tctattccct
     3421 caacacaaac ttttaaattt ataatatgag ctaatttaat aatatattca agaaaagtat
     3481 attcataact attatatgta atattcttaa caaaacttct ttcaattttt attatattaa
     3541 cagacagttc tttaaggtaa tttaaagatg aataccctgt accaaagtca tctattgcaa
     3601 tatatactcc tatacccttt aaactaataa atttatcatt tagtaaattt atatctggta
     3661 tccaacaatt ctctgttaat tccaaaatta aaaattcagg tcttaattga tactctacta
     3721 aacattctac tataaaatct ctaaaaaaat cttcttttaa ctgaatatac gatacattta
     3781 cagatatttt aaagtctgga tttattttat gccattcttt acactgtttt acagcttctt
     3841 ttataatcca cttcccaact ggtataatta aattactctg ttctagtatt ggtataaatt
     3901 caacaggaga tacttctcca taagtactcg aatgccatct taaaagtacc tctgccccta
     3961 ttacctcttt tgtaacagca tttacctggg gttgaaaaaa cagctcaaag tcattaaaat
     4021 tattttcaac gcactctctt agtttttgtt gcattgaaat tacctttagt ttattttcat
     4081 agagctcttg tgaaaaaaac ttaattctat ttttaccact aatttttgct atatccaatg
     4141 caatatcagc atgtttaaat aaatctaaat aattatctcc atcttctgga tacatagcaa
     4201 ctcctgcagt cactgtgcaa tagtacttat ttgattcaat ttcatgctga gtatttgtat
     4261 acatctgtat cttttcatat attttctcta tagtttcttt agtacacata gggtaaaaaa
     4321 atgcaaattc atctccatct aacctataca atcttacatc ttttggaaga tattctaata
     4381 cttcagtagc aattgcacgc aatactttgt caccataaga atgtccatat ttttcattta
     4441 tatttttaaa attatcaata tccatgataa acatagcacc atttttataa attttctctt
     4501 ttataagata attcattcta tgttcaaatt gttctctatt ccacgttcct gtgatagaat
     4561 caaatttatt tttttcacca atattcttta ttctcccgac tagaaatata gtttttgggt
     4621 catccgacac ataaacttta cctctacaag aaatccatac tatttcttca tatttattaa
     4681 tcaacctata ttctaaattg tgctcacctt tttttccatg tagtaattct tgtatatcat
     4741 ctttccatat ttgaacatca tccggataaa caattttact ccaacaattg actaaatcag
     4801 attctatctc tttacttaag ttaaattcat caaaaattgc tgaagaaatt ttaaatttat
     4861 tcctatttat atcccaaaaa aatatataat cttctgtact ttgagataaa agttcaaaga
     4921 aaatttctat atggtctaaa cttaaagagt tcgattcttc tctcatcttt tccccctaca
     4981 atattactat tagtgtagtt taatcaacaa aatattctta tttagattat actattttac
     5041 taattttttt atctcatcaa acacatttcc ccaactttag ttatcttatc aaatctctta
     5101 ctcttaacta agtttttttg agattcataa agtaattttt ctacaatcat tactggtgat
     5161 ttattttcac ctgctaaaac accatgttaa atcatttatt aaatcatttt ctcttaaaag
     5221 tccatttttt attatataaa gagttctcag ttctttattc tataaagctt ttctattact
     5281 cttgaatttg ttttattttt tctcagccta ttctctttgg atttggtatt gcttttctat
     5341 atattttggc aaagaagtgc ctcttcctat aatataagat tttgatattt cttcatcatc
     5401 tataaatttt ttaataggga ttatatcatt ttttatattt atttatataa atattttttg
     5461 cttttaattt tttatctctt attatgtact tacatttaat ttattctttt cagttaataa
     5521 aataggtaca tttttagtac atgaaaatag agttgcttac agggtatcta ggattgatgt
     5581 actatttact agtattacat tttcagccga ttcaacccct cttttgctta cataatttaa
     5641 gtatattttt ctggattttt aaatttatgt actttaaaat ggatatcctt attttcattg
     5701 attttataag aattaataaa aaatacgatt acactacaga attagaatgt caatgtttat
     5761 aaatataaaa taatattttt ta
//
LOCUS       DAMJVX010000200         5751 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMJVX010000200 DAMJVX010000000
VERSION     DAMJVX010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.206"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(234..2066)
                     /locus_tag="PC407_003260"
     CDS             complement(234..2066)
                     /locus_tag="PC407_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498669.1"
                     /translation="MSRMGRGPMGKSMEAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2059..4305)
                     /locus_tag="PC407_003261"
     CDS             complement(2059..4305)
                     /locus_tag="PC407_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498670.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4373..4834)
                     /locus_tag="PC407_003262"
     CDS             complement(4373..4834)
                     /locus_tag="PC407_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2498671.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(5139..5705)
                     /locus_tag="PC407_003263"
     CDS             complement(5139..5705)
                     /locus_tag="PC407_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HDF2498672.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1827 a         1076 c          624 g         2224 t
ORIGIN      
        1 tcacctccaa cattttcctt actacatatt ataataaaat aaacactttg taaagacata
       61 aaattatgtg gctatctcaa aatatagata atcctctttt tcattgttaa aatgataaaa
      121 atacacttta tttcatacac aaaagagtgc cttatgaatt aaaaagttca ttttaaggca
      181 ccctcattta ttttatctta ttaattacat gtctatgcta agatagtttt ttgttacatt
      241 gcttcatctt cttcaaattg agaattgtaa agattggcat agaatccgcc tttctttaga
      301 agttcctcat gatttccttg ttcaactata tctccttcat tcattacaag aatcatatca
      361 gcatcacgta tagttgataa tctgtgggct ataacaaaac ttgttctacc ttccataaga
      421 ttatccatag ctttttgaat caatacttct gttctagtat cgacagaact tgtagcttca
      481 tctaatatta aaattttagg atcagctaaa atagcacgag ctatagtcaa taattgtttt
      541 tgaccttgag aaacattact tgcttcttca tttaatttca tcttatatcc atctggtaac
      601 gtcttaataa atctatgaac atgtgcagat ttagcagcct ctataacttc ttcatctgta
      661 gcattcaact taccatatct tatattttcc ataattgaac cactaaataa ccatgtatct
      721 tgaagaacca taccaaacat ttctctaagc tcactacgat taaaatcttt aatattatga
      781 ccatcaatca gtattgaacc gctattaaca tcataaaatc tcattaacag tttaactatt
      841 gtagttttac ctgctcctgt aggtccaaca atagcaactt tttgaccagg tttaacattt
      901 actgagaaat catttattat agtcttgttt gggttatatc caaagttgac attttcaaaa
      961 tcaattttac cttctaaacc atctattgaa actgcatttt caacaatttg gacttcttct
     1021 tcctcttcta aaaactcaaa tactctttca gaagctgctg ctgttgattg caataaattg
     1081 gcaacctgtg caatctgagt cattggctgt gtaaagtttc ttacatattg tataaatgac
     1141 tgtatgtcac caacctcaat tgttttctta attgcaagcc aacctcctaa gattgatacc
     1201 attacatatc ccaagtttcc aataaacatc ataagtggtt gcattatacc tgataaaaat
     1261 tgagatttcc acgctgaatt atacaggcta tcatttagtt tatcaaactc ttcaattact
     1321 tcatcttcac gattaaatgc tttcactatt gtttggccac tataagtttc ctcaacttga
     1381 ccatttacat ttcctaaata ttcttgttga tatctaaagt atttttgtga tttctttatt
     1441 attcttgaaa ttacaaacat tgatattggt aatattaaaa ctgccactaa tgtcataatt
     1501 ccacttatag ataacatcat tatcaaaaca cctatcatag ttgttactga tgttataagc
     1561 tgtgtcatac tttgatttaa actttgattt aaagtatcta tatcattcgt aattctcgat
     1621 aatatttcac catgcgtctt agtatcaaaa tacttcattg gtaatctatc caattttgct
     1681 gaaatctctt ttcttaaatt ataggatact ttttgagaaa ttccactcat tataaatcct
     1741 tgtataaaag agaacagtgc acttatcaaa taaaggaaaa gtaaaaaggt taagattttt
     1801 ccaattgcat taaaatctat acctacgttt cctccagaaa ctttactcac taacccttca
     1861 aatatctttg tagttgcctt tcctaaaatt tttggaccta ctatagaaaa cgatgcacta
     1921 ccaatagcaa atacaattac taaaattatt gatattttaa atttagataa atatgcaatt
     1981 agttttcgca tagtcccttt aaagtcattt gctttttgtc ctgcttccat agattttccc
     2041 ataggacctc ttcccattct actcattagc tagctcctcc tttgaaagtt gtgataaagc
     2101 tatttgctca taaacttcgc aattttttag aagctcttta tgtgttccta ttccaactat
     2161 ttctccctca tttaaaacaa ttatctgttc tgcatttaag atagtactaa ttctttgtgc
     2221 aactattaga actgtacttt cagcagtttt tgattttaaa gccttacgaa gagctgcatc
     2281 agtttttaag tcaagtgctg aaaaactatc atcaaatata taaacttctg gattttttgc
     2341 tattgctctt gcaatagaaa gtctttgttt ttgacctcca gatacatttg ttccaccttg
     2401 agaaatttca gttttaaaag cttctggttt agaatctata aattctgtag cttgagctat
     2461 ttcagctgct gttctcattt cttcatctgt tgcgtctttt ctaccatatt ttaagttgga
     2521 ttctatagta ccagaaaaca atactccctt ttgaggtaca tatccaattt tttcccttaa
     2581 atcatgttga gaaacatttt ttatattaac tccaccaagt aatatttctc cttcagtaac
     2641 atcaaagaaa cgaggaatta aatttataag agttgactta ccactaccag tacttcctat
     2701 aaaggcagtt gtttctcctg gctttgctgt aaaagatata tttgttaata taggttcttc
     2761 tgcgtctgga tacatgaacg aaacattttt aaattcaaca agaccctttt tatcttcctt
     2821 aaactcttgt gcctctgatt tttctgggtc ttttatgctt atatctgaca ctaatacttc
     2881 atctatacgt gtagctgaaa cggatgctct tggaagcata atagatacca ttgatatcat
     2941 taagaatgac attattattt gcattgtata ttgtataaat gccatcatat ctccaacttg
     3001 cattgctcca gaatcaacac tgtatgcacc tctccaaact ataagaactg tgataaggtt
     3061 cattataagc atcatagcag gcatcataca tgtcataact ctatttacaa aaagatttgt
     3121 tttagttaaa tttttatttg ctttatcaaa tctttcttct tcatgttttt cagtactaaa
     3181 agctctgata actggaatac cagtaattat ttcacggctt actaagttta acttatcaac
     3241 aagcttttga accagtttaa acttaggcat aaccactgag aataacacta caattaatga
     3301 taagatagca actactgcta ctgctataat ccaggccatt gaagtatttg tgtttaaaac
     3361 ctttatcact ccacctatag ccattatagg tgcatagaat actattctaa gcaacataac
     3421 catcaacatt tgtatctgtt gaatatcatt tgtactacga gtaattaatg aagctgtaga
     3481 aaattcattc atttcagtat ttgaaaatga cataactttt ctaaatactc cacttcttaa
     3541 atttctacct acacttgctg ctactcttgc tgctaaaaat cctactgtta ttgtagctac
     3601 catactaatc attgctatac caagcatttt agcaccagta aataatatat aattattttg
     3661 agttttttct gtatcaatac ctattttctc atattctgac cttacatatg aaacagagct
     3721 ttgtgttatc atactctctg gcatactttc aaagcctttg ctcatctctt tagtcaatgt
     3781 atctaactgt tcctttggca ttgctgataa caatttaaat atatcaacat ctccactttg
     3841 agtagcttgt ggtggaagtt tactcattaa ctgagctttc atagctttta cctctttact
     3901 atcgccctca agccctgata ctattaaaat agctttacca aaaataacat ttaaatcatc
     3961 tatagtgtct ttgtctttag tatttaactc ataaagagat tcatctttta gacctgggta
     4021 cttatcttta tacttaacat aatccttttt attcagcaaa gtataattat ctagtacttt
     4081 atccttacta cttttatcca taaataaagt aattttattt aattcactct ctctaatcac
     4141 agatggaaca gagttttcta ctccaccttg ttgtatacct acatttacta tattggatgt
     4201 gtattctggt aaagaaaggt cacataccgc ttgaattacc aatagacaga ctattattac
     4261 tattgataat gccgatttct ttaaatattt aattaattta aacatacatt tcttcccttc
     4321 tgtaatatta attagtctaa taacattaaa ttgaaaaaca ttattttaaa ctttaaaaat
     4381 atttttcatc ttcatgctta tgacatcctg cttttaaatt atctcgtact tgcattagaa
     4441 gtcttctaag tactattttt tcttcttcac taaatcctaa aaaaaactct ctttccaatt
     4501 ccttgttaaa ttcttcaact ttattacata tttctaaacc tttgtctgtc aaaaaaatcc
     4561 tagatactct ttggtccttt tcatcttgtt ttctttccac aagtccagct ttttccatcc
     4621 tctttatcat aactgtagtt gtagatgctt ttactttcct tttatctgct aattcttttt
     4681 gactttgccc atctttcatc tttaaagaaa aaagtatttg gggttgacca tgatataagc
     4741 ctaatttatc aaaataaaaa tgagttctta taaaatatat tcgcattaat tgcaagaaaa
     4801 catcatttaa tttttcttca gaattgctat tcatacttgc cctccttatt ttttatatta
     4861 cttagtcggc taagcattat tttactatac tgcatgcatt ttttcaatat atttataaaa
     4921 aattcatata aatttcattt attaaatatt tttatatcat atagttataa ttttaataaa
     4981 taatagtaag atttttaatt agtcagctac ttatattttt tattgtaaag cttaaaataa
     5041 accttttcat atattttcaa ataaaaaagt tgtcccaaaa taaattttga aacaacatca
     5101 tattcaacag tatcttgatt atataacatt acaatactct acttatcttc atatccattt
     5161 acaacaacgt ctaacttccc agtttcaggt gatataatta gaccatgtac aataatacca
     5221 tctggaatta aaggatgccc ttttattaaa tcaatactat cttttacaga ttcttctgca
     5281 gaatcgaatc catgtaacca ttgttttgta tcaattccag cactacgaag tgtaagtatt
     5341 acatcattag atattcctct atctaaaatt ttaccaagta acttatctgt atcaacatta
     5401 cacataccac atccatgatg ccctacaacc ataacctcat ctacatcaaa ttcgtaaatt
     5461 gcaactaaaa cacttctcat aatacttcca aaaggatgca ttatagtagc tcctgcattt
     5521 tttattagtt tcacatctcc atttttcaaa ttcatagcct taggcaaaag ctcagttaat
     5581 cttgtatcca tacaagataa gactactatt tttttatttg gatgttttga tgtcacatat
     5641 tgttcatatt ctttgttctc tacaaatgat ttattatatt ctaatatctc ttcaagtttt
     5701 ctcatatgta atatcaccct ctttaaaaat ttttttatta ttatatcact a
//
LOCUS       DAMJVX010000201         5742 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMJVX010000201 DAMJVX010000000
VERSION     DAMJVX010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.207"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..1446
                     /locus_tag="PC407_003264"
     CDS             <1..1446
                     /locus_tag="PC407_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2498673.1"
                     /translation="DIKVLILILLTLFSINVFISYLKIKLYEDKINLGLINNSDKFIN
                     ITKDENFLKHVQNYIISNEKENCVMACFDLCRLKLINDIYGYELGDEVLNNILSNLKN
                     YFGKEAIYGKLKSDVFVLIIKLENREQKIPYLVNLIKNKIMILSQIDSFKMDINIEAS
                     IGIYKFNNNEIDIKKAIDNADMARLKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFL
                     AIKNKEFVIHYQPKVDSSTGNIIGSEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIG
                     RWLIQEVFITINKWISEGINVLPVSINLSRVELYKNDLVDFFKLMFNTYNIPKELIEL
                     EITETTALRDVKFISERLYEIKSLGMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLI
                     LDIVTNTKTKFMVKAIINLSHDLNVTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPL
                     DEMNYKKLLTDGSYINLKDTLLDKCMTVEEE"
     gene            1449..1847
                     /locus_tag="PC407_003265"
     CDS             1449..1847
                     /locus_tag="PC407_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2498674.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            1887..3620
                     /locus_tag="PC407_003266"
     CDS             1887..3620
                     /locus_tag="PC407_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDF2498675.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGVGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKKILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            3852..5063
                     /locus_tag="PC407_003267"
     CDS             3852..5063
                     /locus_tag="PC407_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2498676.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            5255..5515
                     /locus_tag="PC407_003268"
     CDS             5255..5515
                     /locus_tag="PC407_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDF2498677.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
BASE COUNT         2428 a          481 c          963 g         1870 t
ORIGIN      
        1 gatattaaag ttttaatttt aattttatta actttgttta gtataaatgt atttatttca
       61 tatcttaaaa ttaagttgta tgaggataaa attaatttag gattgataaa taattcagat
      121 aagtttataa atattactaa agatgagaac ttcctaaaac atgttcaaaa ttatattatc
      181 agtaatgaaa aagaaaactg tgtaatggct tgttttgatt tatgtagact caaacttata
      241 aatgatatat atggttatga gctaggggat gaagtgttaa ataatatttt aagtaactta
      301 aaaaattatt ttggaaaaga agctatttat ggaaagttaa aaagtgatgt ttttgtatta
      361 ataataaagc ttgaaaatag ggaacagaag attccttatt tagtaaatct tatcaagaat
      421 aaaattatga tattaagtca gattgacagt tttaaaatgg atataaatat tgaggcttct
      481 attggaattt ataaatttaa caataatgaa attgatataa aaaaagctat agataatgct
      541 gatatggcaa ggcttaaatc aaaaggacta aagcacatag agtatgtaga atttgataat
      601 gctatggaag aagaaataca atcaattatg aaaatagaga gagatttatt cttggctatt
      661 aaaaataaag agtttgtaat tcactatcaa cctaaagttg attcaagtac aggtaacatt
      721 ataggttcag aggcattgat aagatggttg cacccatcat taggcatggt tggaccaaat
      781 aagtttatac ctatagctga aaaaaatggt ctgataaaca atattggtag atggctaatt
      841 caagaagttt ttattaccat taacaagtgg atttcagaag gtataaatgt cttaccagtc
      901 tcaataaatc tttctagagt agaactatat aaaaatgatt tagttgattt ttttaaactg
      961 atgtttaata catataatat tccaaaagaa cttatagaat tagaaattac agaaacgaca
     1021 gcgctaagag atgtaaaatt tataagtgaa aggctttatg agattaaatc tttaggtatg
     1081 aatatatcat tagatgattt tggagtaggt aattcaaatt ttatcaatct taaggggata
     1141 ccactagata ttataaaaat tgataggtca cttatattgg atatagttac aaatacaaaa
     1201 acaaagttta tggtgaaagc aattattaac ctttcacatg atttaaacgt aactgttata
     1261 tgtgaagggg tagaggatat gcatcaggtc aaagtcttaa gtgaactggg atgtaatttc
     1321 atacaaggtt atgtattttt taaacctttg gatgagatga attataaaaa acttcttact
     1381 gacggaagtt atataaatct gaaagatact cttttagata aatgcatgac agttgaggag
     1441 gagtaaggat gatttcagaa gatgtagatt attcaataag aatcataaga gctatattta
     1501 ataatggaga agataaacca atagatgcta aaatgatatt aaaaagtgaa gatataccaa
     1561 tactaacact atacagactg cttagaaagc tttgtgcaaa caatattctt acttttaaca
     1621 aagaagatgg caagacatac atgttaaatg tgtcattgga tacaataagt ctaagaacta
     1681 ttgtagaagt aattgatgga aaaatatata taaataaatg tacgagatat gtatctaatt
     1741 gcagtaattg cttaaaaggt tgtaaagtaa atcgtgaatt gctagaaata gaaaaagaaa
     1801 tttataaact tttatcgaga aaatctataa gacaaatact agaataaaaa attttcagct
     1861 atgataataa ttggaggttt tcggttatgg atgatatatc tcaagataat tttctactgt
     1921 ctaaagagta tgagaatagc ttagatgtag atactaaaaa agcaagtgga atatattata
     1981 ctcctaaaat aattgtagat tatattgtaa aaaaaacttt aaaaaatcac gatataatta
     2041 aaaatccata tccacgaata cttgatattt cctgtgggtg tggaaatttt cttttagaag
     2101 tatatgatat tttatatgac ttatttgagg aaaatatata tgaattgaaa aagaagtatg
     2161 atgaaaatta ttggactgtt gataacatac accgtcatat attaaactat tgtatatatg
     2221 gtgctgatat tgatgaaaaa gctataagta tattaaaaga tagcttaaca aataaaaagg
     2281 tagtaaatga tttagatgaa tctgatataa agattaatct attttgttgt gatagtctta
     2341 aaaagaaatg gcgatataag tttgattaca tagtgggtaa tccaccctat attggtcata
     2401 aaaaacttga aaagaaatat aaaaaatttc tcctagaaaa atatagtgaa gtttataagg
     2461 ataaagctga cttatatttt tgtttttata aaaaaattat agatatttta aagcaaggag
     2521 gggttggcag tgttataact ccaagatatt tcttagaaag tctatcaggt aaagatttaa
     2581 gagaatatat taaatcaaat gtcaatgttc aagaaatagt ggatttttta ggtgcaaata
     2641 tatttaaaaa cataggggta tctagttgta ttctaacttt tgataagaaa aaaacaaaag
     2701 agacatatat agatgtattt aaaatcaaaa atgaggatat atgtataaat aaatttgaaa
     2761 cattggaaga acttctaaag agtagcaaat ttgaacattt taatataaat cagagattgc
     2821 tatctgacga atggatactt gtaaataaag atgatgaaac tttttataat aagatacaag
     2881 aaaaatgtaa atatagcttg gaagatatag caataagttt tcaaggaata attacagggt
     2941 gtgataaagc gtttatttta agtaaagatg atgtaaagtt gaaccttgta gatgataaat
     3001 ttttaaaatg ttggataaaa agtaagaata taaataaata tatagtagat aaaagtgaat
     3061 ataggctaat atactcaaat gatatagata atgagaatac aaataaaaag atactagatg
     3121 aaattatagg gctatataaa acaaaattag aaaacagaag agaatgtaaa agtggaataa
     3181 ggaaatggta tgaacttcaa tggggtagag aaaagctttt ctttgaaaga aaaaagataa
     3241 tgtatcctta taaatcaaat gaaaatagat ttgctataga ttatgataac aatttttcaa
     3301 gtgcagatgt atattctttt tttataaaag aagagtattt agataaattt tcatatgaat
     3361 atctagttgg aattttaaat tccagtgttt atgataaata ctttaaaata actgctaaaa
     3421 aaatgagtaa aaatatatat gattactatc ctaataaggt tatgaagata aggattttta
     3481 gggataataa ttatgaagag atagagaatt tatcaaagca aataatatca attttattaa
     3541 ataagtctat agataaagga aaagtagaaa aattgcagat aaaaatggat aacttgataa
     3601 tggattcttt aggtatataa gcagttgtaa gtattaaaaa attataatcc tttaattgtt
     3661 tatttaaatt tagaaataaa aagttagcca tataaaaaat attaaaaata tatacttttc
     3721 aatatttttt tgataaaata agaatgaatt tgtgcaattt tatttttctc caataactaa
     3781 aaatagtttt ttgaattaat atttgcttaa gaataaaaat cagaaacgtt tttgatatag
     3841 taggtgataa tatgaaagga ttagttttag aaggtggagg aacaaaagga gcctaccaaa
     3901 taggtgcata taaagcttta agagagcttg gtatagagtt tcaaggagta actggcacat
     3961 caattggtgc tttaaatgga gcatatataa ttcaaaatga tattgaagta atggaagata
     4021 tttggttaaa ttatgactat actcatttta tgaatattga tagagaaacc tatgagaggt
     4081 ttaaaaatgt agatttcact actaaaaatc tcaatgcagt gtttgagctt ataaataaag
     4141 ctagaaaaaa tgaaggtata gatataagcc cattaagaca actaatggag acaacattaa
     4201 atgaagacat aataaggaaa tcaaaaaaag actttggact tgttacggtc tggtgggata
     4261 aaaaaattaa tccatatcca ttgtatatcg aagatatacc taaaggaaga ttgattgatt
     4321 atttaattgc tagttccagt ttgcctattt ttcaaacgaa catgatggat gataagttgt
     4381 atcttgatgg aatgttttgt gataacatgc caataggact cttggaagac aaaggttatg
     4441 aagatgttgt tgtaattagg ttgattgatg attttattgg gaaaatgaat ctaaatcgac
     4501 atcaaaatat aaatataaaa actataatac catcagaata tctaggaggt tctcttaata
     4561 aagatagaga taatgtagaa aaaaacatcc gactaggtta cttagatact atgaaagcat
     4621 atgaaagatg cactggaatt aagtattatt ttgatgtaga gtataaatat aatgaagaat
     4681 attgttttga taaatttaga agtatgagca atgaaacaat acaatatatc tgtaatttat
     4741 tgaatataaa aagagatgct tcacttagaa ctgttatgga aagtgtaata ccaaagcttg
     4801 gtgaggtgct tggattgcca aaggactttt catataaaga tttgttttac tgtatatatg
     4861 aaaagaaact tgaagaaaat aatataaaca ggataaaatt atatgatttt aacaaggtta
     4921 tagatgttgt aaatacaaat attgattcta atagaaattt agcttctaat tttgaagtta
     4981 gacctaaaat tagtattaaa aagaacaaaa acacaaaatt attgacaaat tgtattataa
     5041 aagacataaa aaatcaaagt taaataaaaa acaaaaaaaa atttgtaatt atactcgaaa
     5101 aaagtgtata ttagtagtat aatatttttg tagcactagt gggaacaaaa aatgaccata
     5161 ggacaaaaaa gtaccactta gtaccattaa agtaaaaaac aggaaaacaa ttttaaaaaa
     5221 tataaaatgt aaagtttata ggaggattta aaaaatggaa aaagtagtta gtatcaaaaa
     5281 tgcaagtgga ttacatgcta gaccagcagg aatgtttgtt aagaaggcag ctgaatttaa
     5341 atcaacagta gaggttgtag caaaagacaa aactgtaaat gcaaaatcaa taatgggaat
     5401 aatgagttta ggtttagctc aaggagaaga attaaagtta gttgtaaatg gagaagatga
     5461 agaagtagct ttagctgcaa tggttgaatt agttgaaagt ggatttggag aatagatata
     5521 ataatttttt aaaatttgat atttagaatc taatattact taaagtaaag taacagagaa
     5581 gtattaaaca tatacagacc aacatttgta gatatttaat ttctttgtta ctttcatgtt
     5641 tatttaatca gaagattaag actattgtta attgggtata aaaatataaa tattttagaa
     5701 aatgaaataa gatattttta atcataaatt cagagtaaaa at
//
LOCUS       DAMJVX010000202         5690 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMJVX010000202 DAMJVX010000000
VERSION     DAMJVX010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.208"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            132..632
                     /locus_tag="PC407_003269"
     CDS             132..632
                     /locus_tag="PC407_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDF2498678.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIISGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            689..1675
                     /locus_tag="PC407_003270"
     CDS             689..1675
                     /locus_tag="PC407_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2498679.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1725..2447
                     /locus_tag="PC407_003271"
     CDS             1725..2447
                     /locus_tag="PC407_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HDF2498680.1"
                     /translation="MENFNKNAKIIGIGVEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            2476..4410
                     /locus_tag="PC407_003272"
     CDS             2476..4410
                     /locus_tag="PC407_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2498681.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFIEESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALDGGYFFPVRWIEYGKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            4403..5671
                     /locus_tag="PC407_003273"
     CDS             4403..5671
                     /locus_tag="PC407_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDF2498682.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKIRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDECSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLGYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDIKNTKNDLKKIIFNYKF"
BASE COUNT         2388 a          430 c          971 g         1901 t
ORIGIN      
        1 aatgaatttg gaaaatagtt cgctaaaata atttgttttt ataaaattac tgtttaagtt
       61 tatagatttt gtataaaata aagttatgtt attaattttt ttagaaataa aacttttact
      121 aggagacaga tatgagaaaa aatagtatta atatagcact aactgcagca attacagcaa
      181 tctatgcagt tttaacatta tcattgggat ttataagtta tggaccaata caatttaggg
      241 tagcagagat tatgatgctt ttggcatttt tagataaagg atatatagta ggtcttacat
      301 taggttgttt tttagcaaat gtaataggac catatggtgt acctgatata atttctggaa
      361 cattagccac atttataagt gcaagtatga tttatattac tagaaagtta ggtaaacaaa
      421 ataaaagtac tttagttata gcttctttat ggccaactgt tataaattcg attgtggttg
      481 gtctaatgtt aaatgtattc tttgggcttc cattaattct atctatgata caggtaggtt
      541 ttggagaatt tgtagtagta acaattgtag gagtaccatt tttctgcttt ttaatgaaaa
      601 aatataaaaa tgtcattaat attaaatttt aaatagtgta tagtaattta attaattgag
      661 agtattatta gtgaaaagag gtgtttattt gtcgaaggta aaaagattga ctgatgtttg
      721 gatatatatt aatgataagc aaaagtttac aataaaagag ttgtcagagg aatttaatct
      781 ttcagctaaa acaatacaac gatatttaaa agaattaaat aaaatgggac ttccaattca
      841 agcagaacaa ggtagaaatg gaggttatag ggtgctaaat aatagctata tacctcctgt
      901 tatatttaca gaaaaagaag ttatgattat actatttgca cttaagtcac ttcagattta
      961 taattataag ttaatggaaa tagaaattaa ttctataatg agaaaagtaa gttatgaacg
     1021 taaaagcagt ataaaagtag gtatagaaaa tattaaaaga tacattggct ttgtagctaa
     1081 tgatactaat gatggagatt ttaagagtag taatgtaact gagtttttta gagcttcttc
     1141 agaatctcta attttaaata ttaagtataa gtgtggtaat cagattttgg aaaaaaacgt
     1201 atgccctata ggtatatatt taaataaggg agcatggttt tcaccagcat atgattgtaa
     1261 aagtgataaa ataatattag ttcatataga taatataatt gatataaaaa gaacaggaga
     1321 aagtaaaaaa atacatataa acttggaaga atggttacat gaagtatata acaatttata
     1381 tggaaatagt tcaactacta aaaaatttgg tgaaattatt tatctaaatg tattattaac
     1441 taaagaaggt gtttctaaaa taaaggacag ttcttggaat ttagatatgg agctaaatga
     1501 agatggtagt ggaataatta gtacattcat aaaggttgaa gatatagatt gttatgcttc
     1561 gattttattt tttattggaa ataatgctaa ggttatagag ccaaaatttt tgaataagct
     1621 actttatgat aaggccaatg agttaagata tttttatgaa gaaaatatgg tataatattt
     1681 agaaatgttg aaattaaata attattaagg agatatacgt tataatggaa aattttaata
     1741 agaatgctaa aataattggt attggagttg aaggtattaa tataataaat gaagtagaag
     1801 aaaaaataaa agcaaatatg gatatagaaa aaataaacat aaatcaagag attgaaaaag
     1861 aatatgttag aagtctttta gatggtgtag atatattatt tttgatatat tcatctgaag
     1921 ataaacatat tagagatatt ataaaagctg tttcatatat gtcaaacgaa agaagagttc
     1981 tatctatagg aatggattgt tcagagaaag aaaataaaga agatttagag cttgggagag
     2041 agtttaaaat caataatgat agtatattta aatttgttga tttaatgaat atcatggtag
     2101 agtctatttc tgattcttgc atgataaata tagatattac tgatttaaaa gaagctattg
     2161 taggagataa aggaattaag tattcttttg aagagtttga agatacaaag tcatatagcg
     2221 agatagctga cattttattt gacaggatgg aatatattgg tgaggaattt ataagtaaga
     2281 aaggaattgt atttgttgaa ggaagtccag aattttctat aatggaattg aatgatttaa
     2341 taagtaatat tcagagcaag gtagaagaaa gctatgaagt aatattttct ttatatataa
     2401 aggaaaattt aaatggaaat attagagtag gcttattgta taattagtat attttagata
     2461 ttaaggagag tgtatatgtc tgaaaatata agaggtacag agtattggtt aatggaagaa
     2521 tttttttatc aggaaacaga cacaccacag atttttatag aagaaagcat aattgcactt
     2581 ggatttgata taaaaataaa tttaaaaaaa gtattcagta tggggaaaaa atcagaaaag
     2641 tatattaatg agaattgtaa tgataaagat tttataaaaa agtttatgtc gattaaaaaa
     2701 ggagattatt ttttatataa agcatatcaa aatatagaag aaaaagttaa atgtaatgtg
     2761 tctattggca tagttgaaga agattttgaa aatggatatg aattaagtca tattttaggg
     2821 catacactac cagtaaaatg gatgcataat tttgatacag tttcaaattg taaagtatat
     2881 acaagtgaat tttataaatt aaataataat gatattagtt catatttaga aattattaag
     2941 tcacaagata agaaattatt gagaaaaaga atagaagaaa gtgaaaataa taaatggtat
     3001 ttaaaaaact attattataa aaatgtagat aagtgtgtag atgatgaaga attatttatt
     3061 cagcccatta atctagaaca attagaaagc ctaaaaaata taaagacaga tgattatatt
     3121 ttattgtata atagcgagaa agtaatttgt gaaaatgacc atacaatgaa gagtctattt
     3181 ggcgtatgtc aagttatgga agggttcgat aaagagaaag taagtgcttt ggatggtgga
     3241 tatttttttc ctgttagatg gatagagtat ggaaaggaga atatagacgg aaatgcatgg
     3301 gttatatttg aaaataatag tatagttgaa aagtatgtaa aaagatttac taataaaatg
     3361 ccattaaata ctatacttta tggaccacca ggaactggta aaacatacaa tacaaaacag
     3421 atggtttaca atctagtaag tgaaaccagt ctaagaaaca aatactatac aggttactta
     3481 gattataaaa acaatgtaga attttgtaca tttcatcaat catatggata tgaggaattt
     3541 atagagggtc ttaagtctga tggtgaaggc aattttaagc cagaagatgg gatattaaag
     3601 gatatatcta tagaagcatg ttataatgca ttgaaatttg aaaaaaagat gagctttgag
     3661 ttggaaaaag atagcttgac tttagaaact ataaaagaca ggaaaaagaa attagtatta
     3721 gaaaatatag aatatagtga gagttttaat tttgaagaat cagaaaacta tgttttaata
     3781 atagatgaaa ttaatagagg gaatgtatct aaaatatttg gtgagctaat aacattattg
     3841 gaagatgata aaagacttac taaatcaaat caaactattg tcaaactacc atattcaaag
     3901 gaaaaatttt ctctacctcc aaatttgtat atagttggaa ctatgaacac atcagataaa
     3961 tcaatagcac ttttagatat agctttaaga agaaggttta attttgaaga aattatgcca
     4021 gattatgatt tattgtctat tgtagatggt atagatatta gaaaaatgct atatacaatc
     4081 aataaaagga tagagttttt atatgataga gaccatgtta ttggacaagc ttatctactt
     4141 gctgtagaaa atattaatga tatagtagta gtatttagaa gtaaaataat accacttctt
     4201 caagagtatt tttattatga ttcagaaaaa gtaggtttga ttttaggagg aattggaaga
     4261 aatgaaaaag ataaatacat tgtttataaa gaaaaactta tagcttctga gttatttaaa
     4321 aataataatg cctcaataat ggagagtaaa gttaactatt gtataaaaca aaacatatct
     4381 agtgaggagc taaaaaatat atatgagtaa aaatatatat gttaaggaaa cctatgaatg
     4441 gataagggta ggtaatggtg aaaatgagct tacagaaatt gaatatgaaa aattgcttaa
     4501 atatctagag aacaataatg atgtattaaa aagtaatata atagatatta aatacaaaaa
     4561 aattagattt ataaactatg taggcatcat atgttttgaa aatgtaatac ttgaaatact
     4621 tcctaaattg tcattaagtg ataatcttgt aaaagataga gagatactat tacagatgct
     4681 ttctatatgt aataaaattc caattacaat gaatgaaaaa ataagattga gtttgaaaaa
     4741 ttataatctc ttaaattttt ttgttatgta tttcattgag agtatgcaaa cacagatgaa
     4801 aaaaggtatt tattttgaat atataaataa aatagagaac ttaaatgtta tgaggggtaa
     4861 aatattatta agtacatatg caaaagaaaa aggcatttct ccaatgaaaa taagatgtga
     4921 atatgatgag tgtagtgaaa ataatttctt gaatcaagtt cttaaaaaag catgtatatc
     4981 tattttatgt agaataaatg acaattcaat tcaaggcaag attaaaaaaa ttttaagcta
     5041 ttttcaaaat gtagatttaa tttatataga tagaaaaaaa ctgttgggtt ataaatttta
     5101 taaaaacaat gatagattta aagattgtta tttactagct agacttctac ttttaaatct
     5161 atctatggat aatagtcaaa ataatcaaga ggcattttct atattatttg aaataaatac
     5221 tttgtatgaa gaatatatag gaattttaat caaaagtata tgggacaatt catttagaga
     5281 aacatatata caagataaga gtaaatttct tttgaaaaat gaacaaacag gtaagaaaaa
     5341 ctttaattta agaccggata tagttttata tgacttaaaa aatgaatatg aaattataat
     5401 tgacactaaa tggaaagcta ttgaagtaga ttcaaatgtt ttttataggt caagtgatat
     5461 ataccagatg tatgcatata taacagcata tgaaaatgca aaaagatgta tattattata
     5521 tccttgtatt caaaaagata agaattatag ttcctggaaa ttatcagaat catttaaggg
     5581 taagtttata gaagcaaaaa ctgtgaggtt agatgatata aaaaacacaa aaaatgattt
     5641 aaaaaagata atttttaatt ataaatttta aaaataagaa ttttaaaatt
//
LOCUS       DAMJVX010000203         5608 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMJVX010000203 DAMJVX010000000
VERSION     DAMJVX010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.209"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            240..1067
                     /locus_tag="PC407_003274"
     CDS             240..1067
                     /locus_tag="PC407_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2498683.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            2084..2548
                     /locus_tag="PC407_003275"
     CDS             2084..2548
                     /locus_tag="PC407_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2498684.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLS
                     KEDKRKNFISFINDNKELIGFINLLDEGSYVFFGIGIKPDYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYKSQGFKIVETQVQKTHLGDGEFFVMRYQ"
     gene            complement(2815..3459)
                     /locus_tag="PC407_003276"
     CDS             complement(2815..3459)
                     /locus_tag="PC407_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2498685.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKSLSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPIKRKIR"
     gene            complement(3389..4678)
                     /locus_tag="PC407_003277"
     CDS             complement(3389..4678)
                     /locus_tag="PC407_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2498686.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIDEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINLIWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFIMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(4675..5547)
                     /locus_tag="PC407_003278"
     CDS             complement(4675..5547)
                     /locus_tag="PC407_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498687.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILDSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         2035 a          775 c          698 g         2100 t
ORIGIN      
        1 ataattttaa catgcccata aaatataata cccacaaatg ataaataata ctcaaaatat
       61 ctattgaaat aactatacat ccttaaataa cttaacttaa atctaaacat ttcaaaaact
      121 tcttaaaaat atgtaaaaat ttgatgataa agtagcgatt ttttcaaaaa aaatgtaata
      181 tagagttata tcttataata ttttagagat aaattttatc atagttggga gaaatttgta
      241 tggatttaag caaaaaagta aaaattttag aaaatgcatc agatggcaaa agcgaatttg
      301 aaatattaga atttgaaaaa cttgagggag cttctagccc attaatggca atgggattat
      361 attttgctag agaagctggt ttgaaagcta gacaaattaa gataaaatta aataattcat
      421 caataaaaac agaagctggt gcattatact attataaagg aaatatatct tctgaaacta
      481 agattggtgg tgttggaggt ctttttaaaa aaggaatatc aggagcactt actaatgaat
      541 caatgatgaa acctgtatac aaaggtactg gagaagttta tttggaacct tcttttagac
      601 attatttatt tatgagtctt gataatgatt ctataattgt tgataaagga ttattctatt
      661 gttgctctga tacaatagaa gtaaaagcat ttacacaaaa gaacttctca tctgctttac
      721 ttggtggtga aggcttcttc caaatagaat taagtggtac tggtgtaatc gttctggaat
      781 gtgttgtacc tcaaagtgaa attgtagagt atgaattgaa aaatggagaa gaattaaagg
      841 tagatggtaa ctttgcaata gcaagaacat ctggggttaa tttttctgtt acaaaatcag
      901 ataaatcttt aattggttct gctttaaatg gtgaaggttt cttaaataca tttactggta
      961 atggtacagt ttggctagct ccaactcaag taatgtatga aaaactaagt tttggagcaa
     1021 tgcctactaa tgataatatg aataataaag tttctaagca aaaatagttg ttttataaat
     1081 atttattaaa tcattattga ttttataaag tcactataaa ttttattaga ttattattat
     1141 aaaatcagag tgtggaaatg tttatgtgta ttctttaaat aaaaatttcc acattctgta
     1201 aggttattat acttgatata taaaattcaa tttaggaaca tagtctaaca tttataaaca
     1261 tattaaggta atgatttctc ttggtaatta ttctcatatc ttattctttg attatattta
     1321 tcttattcct agcccttatg tcatattata tttcttatct tattatttat aattagcatt
     1381 aacttatcta aaatcttgta tttttttaat agtaaagttt acatctatat taaaaagttt
     1441 acatctatat taatttcata aaacaatcaa ggtagtcaag agattcaact ttatttgatt
     1501 ttaaaaattt aagtgaattt gttcttatac acatcttttt aaaattatat ctgtagaaaa
     1561 tatcatttag agctataatt aaattattaa attttagaaa atttattaaa aaatataagt
     1621 taaattaata tttttaaaga ttctttttta taactactag aatactaatt tagtagttat
     1681 tgtatctcta aagtttaagc atggatagat atacatttga taatttgcct ataatttacc
     1741 tactacttgg agtattggag caaatacaat atattaataa taagttccta aaaccaataa
     1801 attaactaat acacataaag atattgatgt tgattataca actataagaa acagatttaa
     1861 cagaatttaa tcaagctaag aaattacata tatcgtgatg tttttgaatt agaaggtaat
     1921 tcaaaattag tttatagtct tctgtgaatc caataaaaag tacagggtac aagaattgtt
     1981 agtaaaattt ttaaagattt tatggataga tataaatagt tttacttatt ttgtaaaatt
     2041 taaaatacca ttaattacct taaatatttt aataggagga catatgaatt taaataaagt
     2101 taaatttact gaagaacatg ctattgaagt tactaattgg aaatatgaag gtaaatattc
     2161 aatatacaac ctacctccat gggaggaaat taaaaagaag aatttttcac tatcaaaaga
     2221 agataaaaga aaaaatttta taagttttat aaatgacaat aaagaattga taggttttat
     2281 aaacttattg gatgaaggaa gttatgtatt ctttggtatt ggtataaaac cagattattg
     2341 tggaatggga attggcaaag aaataattag cctaggatta caagaatgta gaaataaata
     2401 tagtactaaa ccaattgtat taaatgtaag aacctggaac atgagagctg taaaatgtta
     2461 taagtctcaa ggatttaaaa tagttgagac tcaggtacag aaaacgcatt taggggatgg
     2521 agaatttttt gttatgaggt atcaatagtt tataacacta gtcatattgc taatatgaca
     2581 attaaaatga aaaaatacat gtgtgtatgt catagcaata agtgtctttg aaatataaat
     2641 ttgagagcct ttgataaaat tcggtataca tatatatttt atactacaga tatataaatc
     2701 tatctacata cataaagaga ttgtaagtag gtttgtgtat ataaaaataa atttatattt
     2761 cataaatgta ctttttattt gtattacctt tattttatct aagctctcat tttattacct
     2821 aatcttcctt tttattggtt ttattaagag agatgcaatt atcaacacaa cacttccaag
     2881 tatcagccaa tatttctcat caaaactacc ttgactcata gacattataa aaaattttcc
     2941 aacatacttg cctttagtca tcatatttaa aattaaatat cctatagata tcatgtaacc
     3001 tatgattgta ttattaaata aatttgagaa aaacattcct aaacttccta taaacatagc
     3061 tgatgcgaaa cttccaataa tatactcaac tactggaaaa ttacatccaa gatatattaa
     3121 aacatttaca aaacctatga ttaataccac tatacctata attgacatca gtatcctaat
     3181 taattgtaca ataacttgag ataatgattt tgaagatact atctcatcaa tttcttttga
     3241 ttgctctagc gaaaatacag gtaccaacat tactatccct attattgcaa cataattttc
     3301 taaacatata gctgttgaaa ttctgtctaa attagctact ccttgtatta gaggaatcat
     3361 acatagcaat ataattgaac atattaaatt agttaaagtg cttaatttga agttttcttt
     3421 tattagaact aaacagtttt gaaaactttg cccaaacatc aagattcccc cttctttttt
     3481 gctcatataa gaatatagct aacaaaacaa gcaccaatgc tcccaagctt atcacaattc
     3541 tattaatcat aatttgattg aagttttgta taataatatc cccatttaat acactattat
     3601 gtcttatcat tagccccatt gttgaataat cacctttgag tcctgtagaa tttatactgt
     3661 aaagccacca aattaattgc acaataattg ctactggtgt atctgttaat atagtaatac
     3721 tcattcctac agctgtaact atcataagag ttggtagtat ccaccataat gtatatttta
     3781 taaatgctaa gttatcaata gatatacctt tatctttagc aaagaaaatc aaaggaagta
     3841 aatctttcat acctaaaatt aatacaggca atatcattat aaagacaagt gcaaaatatc
     3901 ttgataaaac tattttgcat gaagatactt gttttgaata aataatctct ctcattcttg
     3961 aacgcttatc tttaagtgac ataaaaactg ccacaaatac tggaaatata cttaaaacta
     4021 tacctatata atcgcaaaaa attcttgcat acccattact aactttatct ttttgtattg
     4081 tatagttgta attcttcata gcttcatcat aggtaactgg aactactcca aatctttgta
     4141 acttgtcaaa agcatatgca gaccctcctc ctaaaatgtc gtctactctt tgcattattt
     4201 cttcaaatct ttcaagagat actttagaat aatctatttt tatactttca tcagttttcc
     4261 aaattaaatt tatttcgcta atacttttac cagttatttc ttctatatat ttttctatgg
     4321 tcttcgtttt attttggtct aattttatat acttaactac ccctgttgga tacgttgtat
     4381 atgtgttttt ttgatattct ctaattaggt ttatagttgc atttggaatg accttttctg
     4441 ttttcataga ttctttttga ccatatggat attgattgtg tacagagtaa tattcgtcaa
     4501 tattctctac tttttgtgga gcaactaatg gatttccatc attaggaata tttttactta
     4561 catcatttga aactgaactt ccaagttgag aaatatagaa aaaaactaaa actacaataa
     4621 aaatataata taccatactc tttaatatat ttttacattc ttttataaat agattcatat
     4681 attatctcct cccattataa gcatatagcc atcttcaata gttggattac aatttactgc
     4741 tgtctcaaaa ggtttatcat ctgatataat cctaagtata gcctttccac catgagtcaa
     4801 cattcctata actataaatc tactttttat attttcaatg tcttttttat ctacttcaat
     4861 actatatact ttcccttcag ctagttttgc taatgcttct gttgagccat tgtatatgac
     4921 ttttccacta tccaatatag ctacgttttc acatgtagct tctatatctc caacaatatg
     4981 tgtagataaa ataacgattc tatcatttgc aatttcgcat agtaaatttc taaatcttat
     5041 acgttcttct gggtcaagac ctgctgtcgg ctcatccact attaaaactc tgggattatg
     5101 aattaatgct tgtgctatcc caagccttct attcatacct ccagataaag attttacttt
     5161 cacattttga taattagtaa ggtttacttt gcttaataac tttgctataa tttttcttct
     5221 ttttcttagt ggtatatccg ataaaactgc taaataatcc attgctttat atactgtcat
     5281 gttaggatac attgaaaaat cttgtggcaa ataaccaacc atatttctaa tttccttagt
     5341 attctcaata ggaactccac acatatgtat tgaaccttct gtttttgata ataaagtggc
     5401 taaaattctc ataaatgtgg tttttcctgc tccatttctt cctaaaagac caaacatacc
     5461 actgtcgatt tcaagattta tattatcaag agcttgtttt ttcccaaatc ttttactaag
     5521 attggatact gctattttca tttccatata taaatacacc cccagaaata attttataga
     5581 attttatttt tataaatttt tactgtat
//
LOCUS       DAMJVX010000204         5606 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMJVX010000204 DAMJVX010000000
VERSION     DAMJVX010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.210"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            500..1909
                     /locus_tag="PC407_003279"
     CDS             500..1909
                     /locus_tag="PC407_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HDF2498688.1"
                     /translation="MIFSSLIFLFLFFPVTLICYYLANPKYKNVMLLLASIAFYAWGE
                     PKYIFLMILSIIVNYIFGIKVSGSKNKKMWVVLSVIYNISILAVFKYSNFFIDNINFL
                     FNTDITIPEVKLPLGISFFTFQIMSYVIDVYRNDAKVQRKLANLALYISLFPQLVAGP
                     IVRYQTVADSIEYREHNWDKFTQGVNRFVVGLGKKVILANQLGVIADGVFNKSAGDLS
                     RLEVWLGIVCYTLQIFYDFSGYSDMAIGLGKMFGFEFLENFNYPYISQTVSEFWRRWH
                     ISLGTWFKDYVYFPLGGSRVSKAKLYRNLFVVWFLTGMWHGASWTFIIWGLYYGILIS
                     VEKAFLEKFLYKMPRIVRHIYLLLIVMIGWVFFRVDDLPAAIKFIGIMFGAGNYPVIS
                     DAFNVFIIEYWYIIVGAILLSTPIVKVVKSLLIKKNKNTLNSTLYQVTNVVFIVSCMF
                     IVIIMLSSSTYNPFLYFRF"
     gene            1925..3127
                     /locus_tag="PC407_003280"
     CDS             1925..3127
                     /locus_tag="PC407_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498689.1"
                     /translation="MSKKKPRIVAISFLIIVFGFFILNFTLPKKKLSITENRNLAKFP
                     TVENLKKCDFIAKFEEYFNDHFAFRDEMISINTRTQANLNKTKVGNYYLGKDNWILGM
                     FPEILDQKQLSRYEKSINYLSNLSKDMGKNVYFTMMPHKTNMLKHLYPKYVDNMDNID
                     INLKNFKNRLNPDLIKYIDMDEYFLSNYSDKERENLYFKTDHHWTGYGAFEGFKMMAS
                     NMDLGLSKQDLDKHFSSYKKSIITDKKFIGSYNQNIDMLIKENEYVVYMTKDNQDYSF
                     DINGEEKEEKEEKEVYATERDNKEWDYGGAYIRGAKTNILTIKNPKALVDKKILIFRD
                     SYQAPTTLLFADMFKEVEIADPRNINSIYKTYKEIIEESNADIVMFMYNSSGFDSMID
                     SMIDKGIK"
     gene            3328..4311
                     /locus_tag="PC407_003281"
     CDS             3328..4311
                     /locus_tag="PC407_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498690.1"
                     /translation="MKEVFNVHIMQSQNKKIKQKYYLTKDGWLLGNFAKLLSDYHIKS
                     YSKAINNLSKLNRKKDFYFVSLPHKTNMLKHLYPSGIEGKNNIDINKENLEKQLTDIN
                     FLDIDDYFYRSFTEKEREKFFLKTDHHGNSLGAFEEYKFILDKLDLGLSKSEIDSHLK
                     RYSTRYVNDKVFIGSYNKKMGYPLKKKESISYVEISDANYKYFISNGTKNTEINENII
                     KAKLKDKREWDYGGAYMRGCDCHILKIRNSSSLTNKKALIFRDSYQAPMTWMLADTFK
                     ELEILDPRYIKNIDMTYDEIIKNSNANVVLFMYNSFGFAGMIQEMIDKGIA"
     gene            complement(4761..5204)
                     /locus_tag="PC407_003282"
     CDS             complement(4761..5204)
                     /locus_tag="PC407_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498691.1"
                     /translation="MKKNMLYLGIVYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GICMIFQYMYWSKPERAVDYEEKIKNQRIEMNDERRVMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(5363..>5606)
                     /locus_tag="PC407_003283"
     CDS             complement(5363..>5606)
                     /locus_tag="PC407_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2498692.1"
                     /translation="EFIHQLKYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKDLN
                     IREWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         2186 a          694 c          772 g         1954 t
ORIGIN      
        1 atttcaacaa atagtaactt ttttacaaca aatggaaact atggaagcaa taattaaaat
       61 aaaatttata atcaatcata attatttagt ataatgattc gatattattt taaagtattt
      121 gtcatttaaa acaaacttgc gataaaagca tatctgaaag tattctgatt ctaatactta
      181 ataaagagtt ctttttacag ttatagatat aaacaacatc tatcttaata gactgtatag
      241 aggcacaatc aaaggtaaac acatatagtt aagtaatatg tccttgattt ttctttatta
      301 taatagcttt attaattcat tttaatatta catcatttta aaaaaattat atgaaaagtc
      361 aaaatttaca tatagattaa ttaaacttgt aaaaacacat ataaattcat acaattaagt
      421 acttaaaact tgaaaatact tactttgcta tatattttag ataaagatat aattgatttg
      481 gttaagaaag gagtctaata tgatattcag tagtttaata tttttatttt tattctttcc
      541 agtcacactc atttgctatt acttggcaaa tcctaagtat aaaaatgtta tgttacttct
      601 agcaagtatt gcattctacg catggggaga accaaaatat atttttttga tgatactatc
      661 aataatagta aactatatat ttggtattaa agtttctggc agtaaaaata aaaaaatgtg
      721 ggttgtgctt tctgttattt acaatatatc tattcttgct gtatttaagt atagcaattt
      781 ttttatagat aatattaatt ttttattcaa tacagatata actatacctg aggttaaatt
      841 acctttaggt atctcatttt tcacattcca aataatgagc tatgtaatag atgtatatag
      901 aaatgatgct aaagttcaaa gaaaattagc taatttagca ttatacataa gtttattccc
      961 tcagcttgta gcaggtccta ttgttagata tcagactgtt gcagatagca ttgagtatag
     1021 agaacacaat tgggacaaat ttactcaagg tgtaaacaga tttgttgttg gtcttggaaa
     1081 aaaagtaatt ctagcaaatc aattaggtgt aatcgcagat ggtgtattta ataagtcagc
     1141 tggagattta tcaagacttg aggtatggct aggtatagtt tgctatacac ttcaaatatt
     1201 ctatgacttt agtggctata gtgatatggc cattggttta ggtaagatgt ttggatttga
     1261 gtttttagaa aatttcaact atccatatat ctctcaaaca gtatctgagt tttggagaag
     1321 atggcatata tctttaggta cttggtttaa agactatgtg tacttcccat taggtggaag
     1381 tagagtctct aaggcaaaac tatatagaaa tctatttgta gtatggtttt taaccgggat
     1441 gtggcatgga gctagttgga cttttattat atggggtcta tattatggaa tattaatatc
     1501 agtagaaaaa gcattcttag aaaaatttct atataagatg ccaagaattg taagacacat
     1561 atatctactt cttatagtca tgattggatg ggtatttttt agagttgatg atttaccagc
     1621 agctattaaa tttataggta ttatgtttgg tgctggaaat tatccggtta tcagtgatgc
     1681 attcaatgta tttataattg aatattggta tataattgtt ggggcaatcc ttctatcaac
     1741 tcctattgta aaagttgtaa aatcattatt aattaagaaa aataaaaata cattaaatag
     1801 cacattatat caagttacta atgtggtttt cattgtttct tgtatgttta ttgtaataat
     1861 aatgttaagt agctcaacct ataatccatt cttatatttt agattctaat tgggaggtat
     1921 aaacatgagt aaaaaaaaac caaggattgt tgccatttca tttttaatta tagtatttgg
     1981 atttttcata ctaaacttca cacttccaaa gaaaaaatta agtataactg aaaatagaaa
     2041 tcttgcaaaa tttccgactg tagaaaattt aaaaaaatgt gactttatag ccaagtttga
     2101 agaatatttt aatgaccatt ttgcatttag agatgaaatg atatcaataa atacaaggac
     2161 tcaggctaat ttaaataaaa ctaaggtagg taactattat ctaggaaaag ataactggat
     2221 attaggtatg tttccagaaa ttttagacca aaaacaatta agtcgatacg aaaaatccat
     2281 taattattta tctaatctaa gtaaagatat gggaaaaaat gtgtatttta caatgatgcc
     2341 acataaaact aatatgttaa aacatttgta tcctaaatat gttgataata tggataatat
     2401 agacataaat ttaaagaatt ttaaaaatag attaaatcca gatttaatta aatatataga
     2461 tatggatgag tattttttaa gtaactattc agataaagaa agagaaaatt tatattttaa
     2521 aactgaccat cattggactg gatatggagc atttgaaggc tttaaaatga tggcatcaaa
     2581 tatggactta ggtctatcta agcaagattt agacaagcat tttagtagct ataaaaagtc
     2641 tataattact gataagaaat ttataggaag ttataatcaa aatatagata tgctaattaa
     2701 agaaaatgaa tatgtagttt atatgacaaa agataaccaa gattatagct ttgatataaa
     2761 tggagaagaa aaagaagaaa aagaagaaaa agaagtttat gcaactgaaa gagataataa
     2821 agagtgggat tatggtggtg catacattag aggagctaaa actaatatat taactataaa
     2881 aaaccctaaa gcattagtag ataaaaaaat actaatattt agagattcat atcaggctcc
     2941 tacaacactt ttatttgctg atatgtttaa agaagtagag atagcagacc ctagaaatat
     3001 aaactcaata tataaaacat acaaagagat aatcgaggaa agtaatgcag atattgtaat
     3061 gtttatgtat aatagttctg ggtttgattc aatgatagat tcaatgatag ataaaggaat
     3121 aaagtagtaa ataaaaagcc tctattaaat tcaaaattta atagaggctt tttattgttt
     3181 attttttatt gctttggtaa aaataaacta atcaaaataa aagtaaggag atgtagcata
     3241 ttgaacaaaa gacatagtct acctatagta tcagtgatat tattgatggt cttaattatc
     3301 tttaataata accaaaatta tattacaatg aaagaagttt ttaatgtcca tattatgcag
     3361 agtcaaaata aaaagattaa acaaaagtat tatctaacta aagatggatg gctattaggc
     3421 aactttgcta aattattatc tgattatcat ataaaatctt actccaaagc cattaataac
     3481 ttgagtaagt taaatagaaa aaaagatttt tactttgttt cacttcctca taagacaaat
     3541 atgcttaagc acttgtatcc aagtggtata gaaggtaaaa ataatataga tattaacaaa
     3601 gaaaacttag aaaagcagtt aacagacata aatttcttag atatagatga ttacttttat
     3661 agaagtttta ccgaaaaaga gagagaaaaa ttctttttaa aaacagacca tcacggaaat
     3721 tcattaggag catttgaaga atataagttt attcttgata aattagacct tggcttatct
     3781 aaatcagaaa tagatagtca tttaaaaaga tacagtactc gatatgtaaa tgacaaggtc
     3841 tttataggta gttataataa aaagatggga tatccactta agaaaaaaga gtctataagc
     3901 tatgttgaaa taagtgatgc aaattacaaa tattttatta gtaatggtac taaaaatact
     3961 gagattaatg aaaatatcat aaaagcaaaa ttaaaagata aaagagaatg ggactatggt
     4021 ggagcttata tgagaggttg tgattgtcac atacttaaga ttagaaattc aagctcttta
     4081 actaataaaa aggcccttat atttagagat tcgtaccaag cacctatgac ttggatgttg
     4141 gctgatactt ttaaggaact cgaaatacta gatcctagat atataaaaaa tatagatatg
     4201 acctatgatg agataattaa aaacagtaat gcgaatgtag ttttatttat gtataatagc
     4261 tttggatttg ctggaatgat acaagaaatg attgacaagg gaatagcata acaatttgaa
     4321 tcagctcctt tttatacaaa aaatagttct caataattcc taatctatat gataatttag
     4381 tacatctctt taacaataaa aataaaatca caaaaattct tataccatca cttatcttta
     4441 tgataaattg gttgagaaaa aattttataa tttatatgat atgcaatctt aattaattct
     4501 ttatctactg caacctttaa taaaagaaac catccaagac tattaatatg aatttatata
     4561 aaaattactt tcaaataaag tactgcagta tttttattta aagtatctat aatctttaac
     4621 cacaaaaagc cacaaaatac aatgtatact tatggctttt tataaattta tataattgta
     4681 tataaagata tattctaaaa ctgtcttaag aatctaaagt ttaaaaatcc aatattttta
     4741 ttgtgaattt aaacttttta ttacaacttt ttatctaatt tattgtatac aacaacacta
     4801 caaataaatt gaaataaaat aagcaatcca agaacaacta aaacccactt gcttattacc
     4861 attacagaaa atacactcaa tatagaaaca ataaatataa gaattacaag aacataggac
     4921 atgatttgat tagtgatacg tccagcttta tcacgtaaca taactcttct ttcatcattc
     4981 atctctatcc tttggttttt tatcttttct tcataatcta ctgctctttc aggcttactc
     5041 caatacatat attgaaaaat catacatata cctggaccta aagcagctcc agataatccc
     5101 caaataaaag cctcaaatga aaactcagta aacgtagcca ataaaacaag taaaattcca
     5161 caaataaaat atactattcc aagatataac atattctttt tcatttttac tcctcctcat
     5221 aaataaatat ttcttcaata ctcatctgaa cctcccatga ctaaagtcac agggttcctg
     5281 cttcacagaa ttttgcatac taaaatatgt cttactaatt ctccacaggc catccccgta
     5341 gttcctacgg ttcttacata cgttatttat tttgaattgt tattaatctt aatccttctt
     5401 ttaatatatt tatacttgca tttatatctc tatcatgagt ttcattacaa ctaggacaaa
     5461 tccattctct tatatttaag tccttaactt cctcgttttt atacccacat ttattgcata
     5521 tttgtgaact tgcaaagaat ttgcctactt ttacaatttg tctaccatac caattagcct
     5581 tgtattttaa ttgatgaata aattct
//
LOCUS       DAMJVX010000205         5593 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMJVX010000205 DAMJVX010000000
VERSION     DAMJVX010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5593
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.211"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            9..860
                     /locus_tag="PC407_003284"
     CDS             9..860
                     /locus_tag="PC407_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HDF2498693.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(993..2606)
                     /gene="ggt"
                     /locus_tag="PC407_003285"
     CDS             complement(993..2606)
                     /gene="ggt"
                     /locus_tag="PC407_003285"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDF2498694.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2831..4768)
                     /locus_tag="PC407_003286"
     CDS             complement(2831..4768)
                     /locus_tag="PC407_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2498695.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVKTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKMFFKDFEIEKINLYIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKFKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKIIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4755..5522)
                     /locus_tag="PC407_003287"
     CDS             complement(4755..5522)
                     /locus_tag="PC407_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498696.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALKGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTMDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
BASE COUNT         1885 a          905 c          626 g         2177 t
ORIGIN      
        1 tacattcttt gaaaaacatt atttcagcaa aagaacttat atcaaaacta gaaaaaaatg
       61 ataatctagt tgtaatagat tgcagatttg atcttatcaa tagaacttat ggtattgatt
      121 cttataaaaa aggacatatc aaaggcagtt ttatcttaga tatagacaag gatttatcat
      181 ctcctacaaa agaacatggt ggtaaaaatc ctttacaaga ccctttaata ctaaaagaaa
      241 aattagaaaa aatgggtgtt gataatgaca ctacgattgt aacttatgat gatggagatt
      301 taaatggagc ttgcagatta tttttccaat taaaacattt ggggcttaaa aatgtctatg
      361 tactagatgg gggaataaca tctttcgtaa aggaaggtgg agaattggaa gagaaagtga
      421 atataccaag ttgtacaggt aaggaaatta gaccaaatat aaacaatgat ttagttgttc
      481 caatggaata tgttaagtct aaattataca aaaaagatac tgtaataata gattgccgtg
      541 ctaatgagag ataccagggt ttagttgaac ctgcttactc aaaagctggt catataccaa
      601 gtgcaaaaaa ttatttttgt aaggatttaa taaaaagtga ctttgaaaat ggctcactta
      661 aagatataga atttttaaaa aaattcttta aagatttaaa taattatgat gaagtaattt
      721 tatcttgtgg ttcagggata tctgcttgtg ttaacagtct tgctcttaga gaactggata
      781 tacctcacaa gatttatatt ggtagcttta gtgactggat tagttatgat gacaatgaaa
      841 tcaaaactgg tcaagaataa ggctcataat ctatataaaa tcagcatatg atagcttaaa
      901 ttttgtaaag gcgtcagtca ttgtagactt tcgcctttac taattatttc atatactctt
      961 taaatttatt atatatattt tagttgataa tattaataaa ctgcaatatg accatcacat
     1021 cttgattccg tcccacaaat ataactttcc tgttctgttt taaatattat ttgacctcgt
     1081 cccatgtcga cagtatcatg aattactttt atttcatgac ccttttcaat taatgaatct
     1141 attatatgct ttggcatctc tggttcaact tctatttcct ttccttttat ccactgccat
     1201 cttggagcat ctagcgcctc ttgtggattt aatccaaaat caatcatatt tgttaacact
     1261 tgaatatgtc cttgtggttg cataaaagct cccattacac caaatggacc tattgcttta
     1321 tcttctttac ctaaaaatcc aggtattata gtatgataag gttttttaaa tggttttact
     1381 acattgtggt gtttagggtc taaattaaaa ttatttcctc tactatgtaa agcaatccca
     1441 gttcttggta tcacaattcc tgaaccaaaa tttatgtaat tgctctgtat atatgaaacc
     1501 atatttccat ccttatctgc agtacaaagg tatactgtac caccacaata tggctctcct
     1561 gcagttggat acaaagcctc tttattgtca attaataaac ttcttttttt ggcatactct
     1621 tggcttagta attcctgaat ctttaccttc atatgctcta tatcagttac atatgtttta
     1681 gaatctgcaa aagctaattt taaactctct ataattttat gtatgtcttc tacattttct
     1741 atatttcctt ctaattccaa ttcttttaat atgttaagtg ccattaaaac tgttattcca
     1801 tgaccatttg gtgggatttc aaatatttta tatcccttat attctgtact tataggttct
     1861 acccatgttg gataaaaaca ctctaagtca ctctttctta tagccccatt aaatttttta
     1921 gaatattcat ctattttatc agcaagtctt cctctataaa agctttcagc ctgagtattt
     1981 gctatctctt ctaatgtact tgcctgttct tcacatataa atatatctcc agcctctgga
     2041 gctttaccat ctttagaaaa tgtatcaaac catggtttga actcttctcc aataagttct
     2101 ttttcataga gttcatatga ttttttccat accttagcta cattaggaga tactacgtat
     2161 ccctctcttg catagtttat cgctggagat aagcattcca ttaagctaag tttaccatat
     2221 tttttattta attctgacca agctgcgggt attccaggta cagttactgc tccaaaccca
     2281 tattttggca tttctttcat attattataa ttttttaatt ccatattttc tggagcaaag
     2341 ccactagaat ttaaaccata caatcgtttt tcttcctcta tccaaacaag ggcatatgca
     2401 tctcctccaa tcccattgct tgtaggttct actactgcta atgtggcggc tgttgcaact
     2461 gctgcatcaa cagcatttcc accctttttt agtatttcaa gtccagcttg agatgctaat
     2521 ggtgttgatg ttgcaactgc tccattttta gcatacacga catttcttat tgatgagtat
     2581 ttatgttggt aggcattaaa cttcataaat ttcagctcct cgtcattttt tatttgatat
     2641 tctgcttaga tttattatat aacatcaagt aatgtaactg atacaaaatg atataaaata
     2701 tagacttttt cctaacttta attttatctt taaataaata tcttaaactc aattctagta
     2761 ttttctatct gcacttaaat aagttatttt attcaaaaca tatatctaat tcttttgact
     2821 taaaaataat ttattctttt atcatgttta gagcatattt taaagttaaa aaataatata
     2881 taaaatatcc tataaaataa atgactagag ttatgataat tggtacaagt aatgaccttg
     2941 gtataaactt ttgtgctata gacatagcaa ataaattatg aactgtacct acaactaatg
     3001 gcattaaaaa tataattttt aactgttttg atattatttt ctcaatatct ttattatttg
     3061 ctccaagttt tattaacata atatatcttt ccttatcatc atatatattt gataattgtt
     3121 taaataagat tatactacct gtacataaaa gaaatactac agacaaaaat atgcctataa
     3181 acagcattag accacttaat tttatcaagt tattataact ttcaatactt gatgtaaaat
     3241 taaaaggata agtaaatttc atattttcac ttactatatt tttaaatttt aaagtcaaat
     3301 ccagttcttt tttttcattt tttatatcaa ttaatcttat taatctagaa tttccatgta
     3361 attttaactg attaaatacg tcatctttta ctacaattaa atcaaacgta gcttgtgaat
     3421 ttatttctgg ctccaagatg cttttttgta ccttaaacag atttatatca tactcatttg
     3481 tatctttaat atacaagttt attttttcaa tctcaaagtc tttaaaaaac atttttttgt
     3541 atgaatcaga aacatagtac acagattcct ttgaagttaa ttcttcataa ttgttattct
     3601 gatattgtct taatttttta aaatcacttt ctctaataat atctacagaa gttctatctt
     3661 ctatataact atttccattt ttataataca tttcaatatt tatatctttg tctataagtt
     3721 ctattgtttt atcaaagatt atcttattat cttttttata tttatataat aatttgtcaa
     3781 tttcgttatt tacataagaa tttcctaaat ttatggtata actgtattta taattttcac
     3841 ttatgttcct atctatatcg taataaagtg atatggtaaa ccctagcata gttacactcg
     3901 tagctattaa tattgctatt acagccaata cactactatt gctctttaat ttaaatccaa
     3961 gttcagaaaa tgcaattaaa ttctgtccct tataataaaa actttttctc ttcttaacag
     4021 catcaataat catatacata gttgatgaaa aaaataaaaa agttccaggt attattgtaa
     4081 ctaatataaa aactgaaagc ataatatttt cagcaataaa ataactaatt gacattaaat
     4141 aagaaatagc tattaataaa acaccaagta cacattttaa ctttataaat tttttaaact
     4201 tatacttttc tggatattta ttaaataact ctataattct cttatttctg ataactatag
     4261 tatttcttat tccaataact ataaatatta ttaaaaatac cgctagtgtc ttaactaagg
     4321 cctttatact taagttcata tttactacta tcatttcatt cattaatttt acaagcatca
     4381 tagtcataag ctttgaaaat aacagtccta aaaatatacc tattagtatt gcaactatac
     4441 ccatcaaaaa agtttcatat aaaaatattt ttcctatctg atttttcctc attccaagta
     4501 aactataagt tcctacttct cgcctacgtc tattgaaaaa aaatgagttt gaataatata
     4561 taaataaaaa tacaaatacc actattacaa ttgaacttgt attaatactc atcctagttt
     4621 tttctccaag tgaactcaac gcttcattat actctataga tttaaaacta aaaaatacaa
     4681 atacactaaa tacaattgaa ataaagtaca ttaaataatt aaaaaaacta ttctttacat
     4741 tttgcattgc aattctaaat aaagtcattt ttattaactc ctccaattaa ggaaactgta
     4801 tttactattt tattatagaa ctctctgtta ctttctccct ttacaatttc tgtgaatatt
     4861 attccatcct ttataaagag tattctattg caaaaactcg cacttatagc atcatgagtt
     4921 accatcaata ttgttttatt gctgtctttg tttaatttta atagacaatt caacaaatcc
     4981 ctagcagatt tagaatctaa tgctcctgta ggctcatctg caagtactaa tgatggtttt
     5041 gttgctatag ctctacatac agctcctctt tgcttttgtc caccagaaac ttcgccagga
     5101 tatttatcta atatagattc tatatttaat tcttttgaaa tctcttttat tctattttct
     5161 attactgata ctctttcatt ttttaaagaa agaggcaaca ttatattttc ttttattgta
     5221 agtgtatcca aaagattaga atcttgaaat acaaagccta gattatcacg tctaaaatct
     5281 gccaattcat tttgcttcat tttaatagta ttctttccat ttatataaat atctcctgat
     5341 gaaggtaaat ccattgtaga aataatattt aaaagtgttg atttaccagc accggaagac
     5401 cccataatac caacaaattc tcctttataa accttgaaag ttattccttt tagagcttca
     5461 tgttttgaac cttttcttcc atactccttt tttatatttt caacagataa tatttcttcc
     5521 atagttaata atcacctttc atcacaatta gttatttatc aattctcaca aatatattat
     5581 atatattaaa atg
//
LOCUS       DAMJVX010000206         5588 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMJVX010000206 DAMJVX010000000
VERSION     DAMJVX010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.212"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(337..900)
                     /locus_tag="PC407_003288"
     CDS             complement(337..900)
                     /locus_tag="PC407_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498697.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(1059..1772)
                     /locus_tag="PC407_003289"
     CDS             complement(1059..1772)
                     /locus_tag="PC407_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HDF2498698.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            2064..3728
                     /locus_tag="PC407_003290"
     CDS             2064..3728
                     /locus_tag="PC407_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HDF2498699.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDSKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(3835..4734)
                     /locus_tag="PC407_003291"
     CDS             complement(3835..4734)
                     /locus_tag="PC407_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDF2498700.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(4761..5399)
                     /locus_tag="PC407_003292"
     CDS             complement(4761..5399)
                     /locus_tag="PC407_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2498701.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KVGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
BASE COUNT         1934 a          847 c          650 g         2157 t
ORIGIN      
        1 aaatatttat catatttatt atatattaag atatctttat atatttatag ttctgcttat
       61 tcaactatat ttaacattat acgtgtatgt ataaatactt tttatacact gtttgtgcta
      121 tccatcccgc acctaaagga gtgggatttt tgcactattc tgtattatat atatccaaaa
      181 atattataaa aatacaaata ttagcagaaa tttaaaaact gaagaaagta ttctaactaa
      241 gatttacaaa ttcttaatcc tatcgaagtt aaactactat cttcagccta attatctagc
      301 aatatttttt tatatgaaaa actctaaatc aagtttttaa atgtttgcga tttttgatac
      361 ataagaatgt tgtctagaat tatattctga taataaacct tcatactcta aggttaagcc
      421 agataaatta taaaaatcat ttggcgttac ttcaaatgct tgtttattta aaatgtctat
      481 ctcacttttt tctaacatgt cagcaacaaa gtttgaacat acatatttat attcagattg
      541 tctctgaata ttcattagta aatatattaa tgccattgta tcgtaatagt aatcttctct
      601 ataatcacta attaatttta tatttttata tagattttca tattgtaaat tatctacttc
      661 taacgaatat accctacaca cagtgttttt tcttatagca tacaatcctt gatttatatt
      721 ttcttcaaca aatcctccaa taaatggatt ccttggattt agccttccaa aagaatacat
      781 tggttttaga ttttcattta atgatattga cacatgtgta tacagctttt tacttatatt
      841 ccttattaag taagataata tagttcctgt atatgttgtt acaatataga ctttattcat
      901 catttccccc caatctagta actaaatgat attatacact tacaatttaa tatcataaga
      961 tattctaaca taataaatgt ggttttacaa attttgattt taaggtactt taatattata
     1021 taatataata aaaatataaa tataagtttt ctatattttt aaccaacaga tgttttcttc
     1081 aaattaatca gatgatttag tatatcttga acctttccag agtcctttaa atatcctaat
     1141 gccccattta tttgtgattt taaatctatt tctacttcca tatccaaatc aacatttcct
     1201 ttttttatag atatattttt aagtgttatt gggtaaagat aacttttttc caaagtaatc
     1261 ttatttccat taacctcaaa tggaactttt ttattgtagt tacttaatat tttttctagc
     1321 attttatcac ttagtttaaa ttttccaatt ttcacattct caagacttac aactaagttt
     1381 ccatccttta aagtaggtct caattcaagt tcatattgac tatttaccaa accaaatacc
     1441 ttatatggtc ctgctacctt tattttacca tttttcattt caacaaaatt attttcaagt
     1501 tcattaattc catgcttctg catcaatgta tatattaaat ttttaaactc ttcctctgaa
     1561 acagatattt ttccaaccat tctaagtgga ttttttacta cctccataga atttatatat
     1621 gagccttttg ataaatattc ttcatctatg attttattat tttgtgaact aacatcatat
     1681 ctttcttttg gtgtcaagat atatacaaca attcctgcaa gtactaaaac aataacaatt
     1741 aaacttaaca aaatctttgc aacctttccc ataatcgtct cctttacaaa tctgtcatca
     1801 gatttaaaat aaatttagat aactaacttc acataatatt cttaacaata tacatactat
     1861 aatacagtct tttaaaaatt aattcaacca atctaatgtt ttgtaaataa actgtaaaaa
     1921 catgttttta gtataaaagt taatattttg cataatactc ttaattacac ctttattttt
     1981 ttctaatctt ggattaaaat taatattatg caatacagtg tagtgttctg tattataatc
     2041 aaattaaata ggaggtatag aatatgtcaa atgaaacgaa ctcatctaac tttataaaga
     2101 atattattat aaacgactta gaaacaggaa aacatgacag tatcataact cgttttccac
     2161 ctgagccaaa cggatattta catattggtc atgcaaaaag tatatgtctt aattttggat
     2221 tagctaagga gtttaatgga aaagctaatt taagatttga tgatacaaac ccattaaaag
     2281 aggatgttga atatgtagag tccataaaag aagatgtaaa gtggttagga tttgattgga
     2341 acgagttaaa ttttgcatct aactactttg atgaaatgta taagagagct ttaatactaa
     2401 taaaaaaggg taaagcttat gtatgtgatt tgacacaaga ggaaatgaga gaatatcgtg
     2461 gtactctaac tgaaccagga aaagaaagtc ctcatagaaa cagaactatt gaagaaaacc
     2521 ttgatttatt tgaaagaatg aaaaatggtg aatttaaaga tggtgaaaaa acattaagag
     2581 ccaaaataga tatgtcttct ccaaatataa accttagaga cccaattata tatagaatat
     2641 ctcattcaac acaccataat acaggagata aatggtgcat atatcctatg tatgcttttg
     2701 ctcatccaat tgaagatgca attgaaggaa taactcactc tatatgtact ttagagtttg
     2761 aagaccaaag acctctttat gattggtttg ttaaagaatg tgagatggaa aatataccta
     2821 gacaaataga atttgctaga cttaatataa ataatactgt catgagtaaa aggaaattaa
     2881 aacaacttgt agatgagggt attgttgatg gttgggatga ccctcgtgtt cctacaatat
     2941 ccggaatcag acgtaaggga tatactgcag aagcattgcg caatttctgt agtgaaatag
     3001 gggtatcaaa agttaactct acggtagata gtcaaatgct tgattatttc ctaagagaaa
     3061 acttacaacc aaaggctcct cttactatgg gagttttaag acctttaaaa cttattataa
     3121 ctaattaccc agaagataag attgagatgc ttgaaataga gaataatgct aaagatgaat
     3181 ctcaaggaaa gagattagtt cctttctcta gagaattgta tatagaacaa gatgatttta
     3241 tggaagaacc tgttaagaaa tattttagat ttttcccagg aaatgaagtc cgtttaaaag
     3301 gtgcttattt tgtaaaatgt actgatgtaa ttaaggatga aaatggaaat gtagttgaaa
     3361 tacatggtac ttatgaccct gaaactaaga gtggctctgg ctttacagga cgtaaggtaa
     3421 aatctacaat acattgggtt gatgctaaat cagctatacc atgtgaattt agattatttg
     3481 aaccattaat tcttgatgat atacctgaaa atgaaggtaa acatttctta gaacaaataa
     3541 atcctaattc attggaaata ttacaaggtt ttgttgaacc tacacagata aaagattcta
     3601 aaccttttga taaattccaa tttgttagaa atggattctt tagtatagat aataaatata
     3661 caactgacga aaaatttgta tttaatagaa tagttcctct aaaaagttct tttaaaccag
     3721 gtaaataatc ttacaatcaa aaaatgaaga cttaaaatat aaattacttc tactataaat
     3781 aaacttagtt agaagtaatt ttttatttga gtcttcatca tttactttaa atgattaact
     3841 tttttcacta cagattccca taacttctat tccatctttt atgcattcta tgttaagagg
     3901 aaaatctggt cctttttctc cgtcaatatc tgttataagt gaatcttcac attctatatg
     3961 caccttttta gttttaaagt atagtatttc atttccatta tattgatcta aataatcacc
     4021 tctaagcaca cttatcaaaa ctggtattga ttttggtatt ggcattcctt taaaaataat
     4081 tacatccaaa tatccatcat ctatttcagc cttatatgct agatttatat ttcctgctgt
     4141 tttgccatta aaaatcaaca taagatacat atctccatca taatacattt cctcagaaga
     4201 tacttttatc ttaaatttcc tcatatacaa tgcttcttct attcccttta tataataaga
     4261 aattcttcca aatgaatttt ttaagtctgt gtttatcctt tgtgatacat ctgtaaacat
     4321 accagcactt gcaacattta taaagtactt attattgact ttccctatgt ctattttctt
     4381 tggttttgaa tttatgattt catttatagc ttcttttata tcaaatgaaa tttgaagagc
     4441 atttgaaaaa tcatttgcag ttcctgtagg caaaattcca attggaattt ttatgtctaa
     4501 ttctttcatt gcatttacta ctaaatctac agtaccatct ccaccagcta tcaatatgtg
     4561 ttcataagaa ctatcaactt ccttaaatgc attctttaca ggttctttaa tagttaatct
     4621 atagggtatc aataaataat ttttttcttg atataattct atgatagtgt ctaaatttga
     4681 taatatcttt ttttcacctg aattaggatt gtatataagt tttacttttt tcattatttc
     4741 tccttactca ttttattctt ttagtatcca aaatcttcat ttaaaaaaat cacatgtatt
     4801 ctttctggtg ttaattgtct ttttataagt gtataactac agcatattct tcctggactt
     4861 gaacagtcta tacatatacc agtagtctta catccagtat ttgtacttaa tcttttacaa
     4921 ttggctggag cagctattgt ttttgttctt tttatagctt catctacatc tttaactatt
     4981 ttatttactc ctgctataac tattacttta tcaggtccat atatcatagc agcaactcta
     5041 ttcccattcc catctaaatt gtaaagttct ccatcctctg ttatcgcatt agtacttgta
     5101 aaataagcat ctgcagaaaa tgactttcta tatatttctt tcatttcttc tggtttgata
     5161 tcttttgcat atctatcaag aaaattatat ttttctctta taaaatcaat cactcctgtt
     5221 tcaaataagg tcatagaacc tccacatcct actagagaac ctactttaac tatttcttca
     5281 atttttttaa gaagctcttc tttgttactt actaaatacc ctttcatatt attgttttcc
     5341 agagatttta ttgttttttc tattcttttt tcattaagcc aatttaaatt ttgatccatt
     5401 ttaacttccc ccttattttt aatatgtact taattattta taaactttat tgtctttata
     5461 agtttatttt agtacatcta aaaaaaatac aatataaaac ttaaatagtt ttatataatt
     5521 ttttccaaaa taacttactc aatcaaaaaa ggaaagtaga tgtactattc tactttccct
     5581 atattgta
//
LOCUS       DAMJVX010000207         5474 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMJVX010000207 DAMJVX010000000
VERSION     DAMJVX010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.213"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            93..1274
                     /locus_tag="PC407_003293"
     CDS             93..1274
                     /locus_tag="PC407_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2498702.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQVDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(1416..2333)
                     /locus_tag="PC407_003294"
     CDS             complement(1416..2333)
                     /locus_tag="PC407_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498703.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            2586..3380
                     /locus_tag="PC407_003295"
     CDS             2586..3380
                     /locus_tag="PC407_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498704.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            3381..5441
                     /locus_tag="PC407_003296"
     CDS             3381..5441
                     /locus_tag="PC407_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2498705.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFACYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEELFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         2093 a          624 c          907 g         1850 t
ORIGIN      
        1 aatagaatgc ttatagtggt aaaattaatt attagaatat tagaaataat aaataaataa
       61 tacattgtac ttaaaagggg gagaaattta agatgaacaa aaaatatatt aaagcaggga
      121 atttaattga tggaacagga aatagtatta tgcaaaataa gggtataatt attgatggag
      181 atactatagt tgaaattgaa gatataagag atgatttaaa tggatatgat gtgtacgatt
      241 acttagataa aactgttatg ccaggcatta tgaattgtca tgttcacttg acaatggagc
      301 cagttggaaa tccaaagata tattatgata atgtatcaga tgtagagctt gtagtaaata
      361 cgataaaaca agttgatgct tatttggatt caggagtaac atatattaga agcttaggtt
      421 gtcccaaata tgtggatgta caacttaaaa atcttataga caaagggtct atagatggtc
      481 ctggtatagt tacttcaggt ccagttatat gtatgacagg agggcatgga tattattttg
      541 gtattgaaag tgatggagtg gatgaatgta gaaaatcagc cagaacagtt ttaaaaaatg
      601 gagtagattg tgtaaagata atggcaactg gaggtgtaac aactgatgga gttgagccag
      661 ggtcaccaca attaactttg gaggaaatga aggctgctgt agatgaagct ataaaagcag
      721 gtaaaataac tgccacacat gcacaaggaa gaactggtat aaaaaatgca gttcttgcag
      781 gaattacttc tatagaacat ggtgtttatt tagatgatga aataattgat ttgatgcttg
      841 aaagaggaac ttacttagtt ccaactgtgg ctgcaccata ttttattcta aatgcaggaa
      901 aagaaagtgg aataactgaa gctactctta gaaagtgtga gattgttgcc attccacata
      961 aagaatcatt cctaaaagca tataaaaaag gaattaaaat tgcagttggt tctgatgcag
     1021 gaacttctta taatgaacat ggaaaaacat attatgaaat gaagctaatg tcagattatg
     1081 gtatggataa tatggatata atagttgctg ctacaaaaac agcatctgaa cttttaagaa
     1141 ttgataaaaa ttatggaact ctagaaaaag gaaagaaagc agatgttata gttgtaaatg
     1201 gaaatccagt agagaatata gacgtacttg cagatgtgtt agcagtattt aaacttggta
     1261 aaaaagttag ataaatagta taaaaataaa aaagagtgtc taaagaaatt tttaaataat
     1321 tatagacact cttttttatt agtaataatt aaatctttta tactttagaa aatagattct
     1381 attttatatt tagatataac tctttaaaat cattcttatg caatataatc tatagaatct
     1441 acacatacaa agtatattct tgaatcatta gaatttccaa gtactataac atcatttttc
     1501 ttacctagaa cttcaacacc agttagaacc aaactaccag ctactacagt aacttgatta
     1561 tttatatttg acatataaag attatctagg atacctttat tacaacagca atcatcacaa
     1621 tcgcagttac aattacattc tttgtaatgt tttttatcaa gatagtaacc taaagatgct
     1681 atgaaatctt cataatctgc aggtgtaata tcaaatgcaa cagctttaag attacacaat
     1741 gatgctaaat ctacgtttgc taatggagta gatgcagcaa gtataaaatc aattatagca
     1801 gcaagtattg catctaatat agccccaagg tctatcgtag gaggaataac tgcatcaata
     1861 agtgcaatta attcagctac tcctggtata gttcctatag tattaattaa tccctcaaga
     1921 gttaaaggga ctggaatagg ataagatact ctaccagcta tatctattaa atcacagtta
     1981 caagcagaaa atctttcaaa agtaccatca agtccagata aattatcttt tggaggagct
     2041 gaaagatcta tacctaccaa attagcacct actataaaga aatctgagat aaaagcaaat
     2101 tgattaaagt ttacaaaagg tcttagagca tcatatctta gaagttctaa ggcctctctc
     2161 atactttttt tacaacaaca ttgttttatt ttaggttctt tatatttatt tggataatta
     2221 tagtatctat cagctacttg ataattttca tagtatcttt catttgtatt tggataatct
     2281 tcctcatatt cttgtgtaaa tctaaagtcc tctctacatt tattattttc cactaaatac
     2341 actcctttta tattttaatt taaatgtata gataattaag cttatctata tagtaatatt
     2401 atatttataa tgtaaaaaaa tgttacaaaa gatattataa acgaataaaa aataataatt
     2461 tgtcagataa acttgtaaat ttttatgaat aatccattct aaaacaaaaa actgtgtata
     2521 aaaaaggttt atatgttata tttatataaa tataacatat gaacgagaaa ggagtggttt
     2581 gataaatgat tgaggtagat aaattgtgta agtcttttac aagggttgta aaagatgata
     2641 agaataaaag ttcaataaga aagctaaaga aagtaaaaac taaaaaagaa gaattttttg
     2701 ctgtaaataa tgtttcattt aaagttatgg aaggtgaaat tgttggaata ttaggtccaa
     2761 atggtgcagg aaagactaca ttacttagaa tgttaggtgg catattaaca ccaacatcag
     2821 gaagtataaa tatttctgga tatgattatt caatagatag aaattcagca aaaaaagaaa
     2881 taggttatct ctctggaaat acaaaactat atggaaggct ttctccgaga gaacttctaa
     2941 caacttttgc aagtctatat gagatgtcaa aagaagatat agaagaatca attgaaaata
     3001 ttgtaaaaat aatggatatg agtgaattta tagataatag aattgaaaat ctatcaactg
     3061 gtcagactca aagaacatct atagcccgtt gtttaataca ttctccaaaa gtatatattt
     3121 ttgatgagcc aacactaggt ctggatgttt taagtagtgc atctattata gattttatga
     3181 agaatgaaaa actaagagga aaaactgttc tttactcaac tcattatatg gaagaagcag
     3241 aaactttatg tgacaagatt tttatgattc acaatgggaa aataattgca tcaggaacac
     3301 cacagagttt aaaagaggaa tctggagtaa ataatttgag agatgtattc ataaaacttg
     3361 ccagaaagga ggacatttaa atgagaagta agattgtaaa atacatattt aaaaaagaaa
     3421 tgttagacat attgagagat aaaaagactt tatttatgat gattgttgtg ccacttcttt
     3481 tatatccttt acttatgtta ttaatgattc aagtaatgaa tatgtctgca agtagtatga
     3541 atagtaagga cattaatata gcttttaata ataaacctaa taaggtgtta gtatccatga
     3601 ttgaagatga taatttagaa aaagatacta aaaatacttc aaatgataaa ggcaaaataa
     3661 gagtgttaga tgtagataac tataaaaagt cattagaaga aaataaaatt gatgcttata
     3721 taaagatgga agaaaaaaaa tcatctataa attatcaaat atatataaat tcttctcaag
     3781 aaaattcttc taatgcacta aaaagaattg aaactgtttt ggataaatat aaaagattta
     3841 ccatagaaaa aactttatct gaaaaaggat tagatgtaca agctacttta gaaccaataa
     3901 catatagcac tgtagatgtt gcaaagacgg aagaagtggc aggatacttt ttaggtcaga
     3961 tacttccatt tatattaata ataggagttt tactaggttc aatatatcca gcaatagatg
     4021 ttatggctgg agaaaaggaa agaggtacat tagaaacatt atttacactc ccaatatcaa
     4081 acttagaact ggttatggga aaatatatgg cagtttctct tagtgcaata gtaacagcaa
     4141 ttttaaacat tttatctatg ggactgacta tggcatttat tttagttagt ggaggtatta
     4201 gtagccaatt tggctttgga aactttaatt atggagactt agtcttacca attataacta
     4261 ctttaatttg catatgtctt ttttcaatgg tagtttcagc aataagtatg tgtgtatgtt
     4321 cattagcaaa aagttttaaa gatgctcaaa actacataac acctcttatg ttaattatta
     4381 tgataccatc atatgtttct atgataccaa atatagtatt ggatagagtg acagctgtaa
     4441 tacctgtagt aaatatatct ttacttataa aaagtgtatt atcttttaaa agcgatttaa
     4501 atttaatagc cttagtttta gcatcaaatg tagcctttgt tatattggca atcatactac
     4561 tgtctaagat gttcaattct gaggaaatac tttttggtaa tagtaagagt ttttctttct
     4621 tagagaaaag aagtaatata aaaaaagggt ctataccaac tgtaagtgat ggtgttattt
     4681 tatatgcagt aggattagtt ctattgattt atgttggttc tttagttcaa attaaatttg
     4741 gtatggcagg tattataatg acacaattca tgataatttc tttaccatta ttatttgcat
     4801 gctatattaa agctgatttt aaagaggtat ttagcttgac agttcctaaa ttaaagcatg
     4861 tgtttgggag cattgttctt tggattggtg gatacatact tattatgatt ataacacagc
     4921 taattttatt cttatttcct caaaatatgg aagttgctga aagtttaaat aaggctcttt
     4981 ttatcaaaga tagtgtttta ctgaatttac tagcaatagc tttattacca gctatatgtg
     5041 aagaactgtt ttttagggga tttatatttt cttcatttag taaatcaaaa gataaaaata
     5101 agtctatcaa actcgctata atatgtagtg gggtactatt tggtattatg catatggatt
     5161 ttattagaat aattccaaca tcaatattag gtattatatt tgcatacagt gtgtataaat
     5221 ctggttcaat atttgtatca atgttattgc attttttaaa taatagtgta gcagttgtgt
     5281 taaatcatta tacaactgga aatataacca acttatataa atttgttgaa gttgattttt
     5341 cgaacttaaa tgtacttaaa tttgccttaa ttcttctaat aagtcttata ttaattatgt
     5401 taggattaag agttttagat agaaagtctt taagaaatta agtataaagt tattatatag
     5461 attaaaatat taat
//
LOCUS       DAMJVX010000208         5463 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMJVX010000208 DAMJVX010000000
VERSION     DAMJVX010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.214"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            105..2606
                     /locus_tag="PC407_003297"
     CDS             105..2606
                     /locus_tag="PC407_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDF2498706.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNVLAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2766..3434
                     /locus_tag="PC407_003298"
     CDS             2766..3434
                     /locus_tag="PC407_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2498707.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3418..4695
                     /locus_tag="PC407_003299"
     CDS             3418..4695
                     /locus_tag="PC407_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF2498708.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEKSELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            5096..5317
                     /locus_tag="PC407_003300"
     CDS             5096..5317
                     /locus_tag="PC407_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498709.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT         2304 a          554 c          970 g         1635 t
ORIGIN      
        1 aataaaaata tagatttcta aataagtaat ttatcaatat tacatatcaa aataaaacgt
       61 tacaaattag ctgaaaatat attgaaagaa aggaagattt tattatgagt tcaaataaag
      121 tggtagaaag ttataaaatt acaggtatga catgtgcagc ttgtgcaaag gcagtagaaa
      181 gagtaactaa gaagatggat ggagtatatg accaaagtgt aaatatagct actgaaaaac
      241 ttaaaataga atatgataat tctaaggtta attttgatga tataaagcag gttgtagaaa
      301 aagcaggata tggaattata aaggaagaaa gtaataaaaa aattgatatg aaaattgatg
      361 gaatgacatg tgcagcttgt gcaaaagctg tagagagagt ggtaaaaaaa ttggatggag
      421 tagaaagtat tagtgtgaat attgcaacag ataaagctaa tatagactat gacccatcaa
      481 aagtaaaatt atctcaaata aaagcagcta ttgaaaaagc tggatataaa ccaatagaag
      541 aggtaagaaa taaggttgat gtggatgaag ataaattgag aaaagaaaga gaaatgaagt
      601 ctttatttgt taaatttata gttgcaatag tatttgcagt acctctgttt tatatagcaa
      661 tgggacctat gataataaag cctatagggc catggccttt gccagaaatt ataaatccta
      721 tgacaaatac ttttaattat gcattaattc agttaatttt agtaatacca gtaatgatag
      781 ctggttataa attttatata aatgggttta aatcactttt ttctttaagc cctaatatgg
      841 attctttagt tgcaatagga acattagccg catttttata tagtctatat acgacactac
      901 aaatagccaa tggacaaatt caaggtatgc accatcatca gttgtattat gaaagtgcag
      961 gaataataat agcacttatc ttacttggta aatatttaga atcaaaatca aaaggaaaaa
     1021 catcagaagc aattaaaaag cttatggggc tacaacctaa aactgcaata gtgttagttg
     1081 atggtaaaga agttgaaact cctatagaag aagtagagat aggagatata ctattagtaa
     1141 aaccaggaac taaaataccc gtggatggag ttgtaataga aggatataca tcagttgatg
     1201 aatcaatgct tacaggtgaa agtataccag tagaaaaaaa tgttggaagt aaggtgacag
     1261 gagctagtat taataaaaat ggtgttatta agtttaaggc tgaaaaaatt ggtggagata
     1321 ctgctcttgc ccaaataata aagttagttg aagatgcaca aggtacaaaa gcacctattg
     1381 ctaaacttgc cgatactgta tctggatatt ttgtaccaat agtaattgca atagctgtag
     1441 ttgcatcatt attgtggttt ttaataggag gaaaagatat agtatttgta cttactatat
     1501 ttatatcagt acttgtaatt gcttgtcctt gtgcattagg attagcaaca ccaactgcta
     1561 taatggttgg gactggaaag ggcgcagaaa atggaatctt gataaaaggt ggagaggcac
     1621 tagaatctgc acataaagta aatacagtga tatttgataa gacaggtact ataacagaag
     1681 gaaagccaaa agtaacagat atagtattaa ataataatgt aaaagaagaa tatcttatca
     1741 agattgcttc aagtgcagaa aaaggttcag agcatcctct tggagaagca atagttaagt
     1801 atggagaaga gaagaatata aaatttgaaa aagttgataa ttttaaagcc ataccaggtg
     1861 ctggtataca agttactatt aatgatgaaa gtatattact tggaaataga aagcttatga
     1921 atgacaataa tataaagctt ggggatttag aagaaaaatc aaatgtattg gcaagccaag
     1981 gtaaaacacc tatgtatata gctgtagatg gaaatttatc aggtataata gcagttgcag
     2041 atgtagtcaa agaaagtagt aaaaaagcaa tagaaatact tcatgatatg ggaataaaag
     2101 ttgcaatggt aactggagat aatgcgaaaa ctgcaaatgc tatagcaaat caagtaggta
     2161 tagatatggt tttggcagag gttttaccag aagataagtc aaaagaagtt gaaaaactac
     2221 aaaatcaagg aaagtttgtt gcaatggtag gggatggaat aaatgatgcc ccagctcttg
     2281 caaaagcaga tatagggata gcaataggaa gtggaacgga tgtagccatt gaatctgcgg
     2341 atatagtact tatgaagagt gatttaattg atgttccaac agcaattaaa cttagtcatg
     2401 aaactataaa aaatataaaa caaaacctat tttgggcatt tggatataat acgattggta
     2461 tacctgtagc agcaggaata ttatatgtat ttggaggacc tttattaaac ccaatgattg
     2521 cagcagcagc aatgagctta agttcagttt cagtcgtatc aaatgcactt agacttaaaa
     2581 acttcaaggc atataagaga gattagatta ataaattaaa taaaatgtat ccaaaagagc
     2641 tatctttaaa ttaaaaggat taatttacaa attaagatag ctcttttttc atgtaaaatt
     2701 cacaacaata agataaaata taagtaatat acgaaatttt aaataaaata gaaaggattg
     2761 atagaatgaa ttcgtcaata cttgtaattg aagatgattc aaatatacaa gagttaatat
     2821 cagaattttt aagtgcagaa ggttatcaag ttgatacagc aaatgatggg ttggaaggta
     2881 tacaaaagtt taagcaagga agttatgatt tagtaatatt agatataatg atgccaaact
     2941 tagatggtta tggagtttgt aaaatgataa gaaagtcatc aagcgttcca attatatttt
     3001 taactgcttt aaatgatgaa ggagaccaac ttaaagggtt tgacttagaa tgtgatgatt
     3061 atataacaaa accattttca tttaatcttc ttataaaaag agtagaagca attttaaggc
     3121 gaagtaacaa gactataaat gacaaattta tagtttttga aaaattaaaa ttagatttaa
     3181 acacatatat tgcagagata gatggagaac ctatagaact tacactaaaa gagtttaata
     3241 tattaaaagc tttgatagaa aagtatccac aagttataac tagggaaggt cttttggata
     3301 gtatatgggg ttatgactat tatggagata ctaggattgt tgatgcacat ataaaaaata
     3361 taagaaaaaa aatatcgtta ccatatatta aaactgtaaa aggaatagga tatacacttg
     3421 agaaggatat ttgataagtg ggaaaagtta agtataaagt ataagttatt ttcaataacg
     3481 acatcattat taatagcgtt agctttaata atatatctaa ttttatattt tttattgcca
     3541 tcatattatc atgagtataa aattgagtct cttcaagaaa gtttaaaatc tttagtagat
     3601 agttcaattc atttcgatac atatacttta gaagaaagac tttattatat ggctaaagac
     3661 caaaatttgg ctattttatt aaaagataac caagggaaaa ttgtatatgg taagaatgaa
     3721 gtagttattt taagatatag taagtacatg attaatagtc ttgaggatga gtatagaact
     3781 tctataccta tatatactaa agatgcaaaa gatggtccat atactttaga actcgttatg
     3841 ccattacaac caattgacga agcaaacgaa gttataagaa aactaatgcc atacatcata
     3901 tcaatagcaa tattgatagc gattattgga gcatatatat attcaattgt aataacaaag
     3961 cctcttataa atataataga gagtgagaga gaacaggaat atagaaggaa ggattttgtt
     4021 gctacaatat cacatgaact aaaaactcca ataaccataa taagtggtca aattgaaggt
     4081 atgatttata gtgtaggtaa gtataaggat agggatactt atctgaaaaa atcatatgaa
     4141 tgtacacaag aattaaaaga tttagtaaat gaaatgatag aggtttcaaa atcagaaata
     4201 cttgaaaaag atttgaaatt ggtatcaata aatataagtg aattattaaa tagacttgta
     4261 aagagacaag tatttttaat agaagaaaaa catatgaaaa ctattttaaa aatagaagaa
     4321 aatttggaaa ttaaagcaga ccaagagaga ataactaagg caatcaataa tataataaat
     4381 aatgccataa aatattctcc tgaaaaatca gagcttataa ttagactcta tgataaaaat
     4441 aaacgaataa gtaaaaagac ttctaatcaa agagtggttt tagaaattga aaatactggt
     4501 gtaactatcg agaaacgtta tttagaagag atatttaatc cattttatag aatagaaaaa
     4561 tcccgttcaa gaaaaactgg aggaagtgga cttggacttt atatagtcag tcaaattttt
     4621 aaaagtcatg ggtttgatta tagcataaaa aacaaagaaa actctgtagt attcacagta
     4681 gaatttaaaa attaattact tattataaag aatatataac tttcaataat agttcatttt
     4741 aaaatcacaa aaaattcata atgaaactgt attatataaa tataaagaag cttaaaacca
     4801 taaaatacac caactataat gatgagattt ccttacatcc tccatataag tcatcattat
     4861 agtaacccaa caattaagtt actatgaatt taaaaaataa caccgagcat ataatctatg
     4921 cttagtgtta tttttatttt gaaataaaaa atatttaatt tattgaaatg tatagattta
     4981 gacagtttaa actatattta agacttaaaa attataattg atattacaaa taaatatatg
     5041 tactttggga aaaataaata taggaagtat tatatttaca gaggaggtag ttttgatgga
     5101 tagattaaaa agagatatga aaaatatgaa agaagattta aaagaaggtg ttaaaaagac
     5161 agcttatgtt atgaaaaata cagctgaaga tttgaaagat gatgcacaag gtgctatggt
     5221 aaacttagaa atgaaaaaag atgagatgat ggaaagatat gaacaaaaga aattttctaa
     5281 agaattagag aagcaaatga agaatgaaat gaaataagct tttatttatg aataacaaat
     5341 tattgtattc atataaaaag tagccagtaa tattaaatag tttatttaag gaaatagact
     5401 ttctaaaaat aactgtatta gatttagaaa gtctatttca aattagtttt ataaatagac
     5461 ata
//
LOCUS       DAMJVX010000209         5451 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMJVX010000209 DAMJVX010000000
VERSION     DAMJVX010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.215"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(122..2752)
                     /gene="cdtB"
                     /locus_tag="PC407_003301"
     CDS             complement(122..2752)
                     /gene="cdtB"
                     /locus_tag="PC407_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094607992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin binding subunit
                     CdtB"
                     /protein_id="HDF2498710.1"
                     /translation="MKIQMRNKKVLSFLTLTAIVSQALVYPVYAQTSTSNHSNKKKEI
                     VNEDILPNNGLMGYYFTDEHFKDLKLMAPIKDGNLKFEEKKVDKLLDKDKSDVKSIRW
                     TGRIIPSKDGEYTLSTDRDDVLMQVNTESTISNTLKVNMKKGKEYKVRIELQDKNLGS
                     IDNLSSPNLYWELDGMKKIIPEENLFLRDYSNIEKDDPFIPNNNFFDPKLMSDWEDED
                     LDTDNDNIPDSYERNGYTIKDLIAVKWEDSFAEQGYKKYVSNYLESNTAGDPYTDYEK
                     ASGSFDKAIKTEARDPLVAAYPIVGVGMEKLIISTNEHASTDQGKTVSRATTNSKTES
                     NTAGVSVNVGYQNGFTANVTTNYSHTTDNSTAVQDSNGESWNTGLSINKGESAYINAN
                     VRYYNTGTAPMYKVTPTTNLVLDGDTLSTIKAQENQIGNNLSPGDTYPKKGLSPLALN
                     TMDQFSSRLIPINYDQLKKLDAGKQIKLETTQVSGNFGTKNSSGQIVTEGNSWSDYIS
                     QIDSISASIILDTENESYERRVTAKNLQDPEDKTPELTIGEAIEKAFGATKKDGLLYF
                     NDIPIDESCVELIFDDNTANKIKDSLKTLSDKKIYNVKLERGMNILIKTPTYFTNFDD
                     YNNYPSTWSNVNTTNQDGLQGSANKLNGETKIKIPMSELKPYKRYVFSGYSKDPLTSN
                     SIIVKIKAKEEKTDYLVPEQGYTKFSYEFETTEKDSSNIEITLIGSGTTYLDNLSITE
                     LNSTPEILDEPEVKIPTDQEIMDAHKIYFADLNFNPSTGNTYINGMYFAPTQTNKEAL
                     DYIQKYRVEATLQYSGFKDIGTKDKEMRNYLGDPNQPKTNYVNLRSYFTGGENIMTYK
                     KLRIYAITPDDRELLVLSVD"
     gene            complement(2805..4196)
                     /gene="cdtA"
                     /locus_tag="PC407_003302"
     CDS             complement(2805..4196)
                     /gene="cdtA"
                     /locus_tag="PC407_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin enzymatic subunit
                     CdtA"
                     /protein_id="HDF2498711.1"
                     /translation="MKKFRKHKRISNCISILLILYLTLGGLLPNNIYAQDLQSYSEKV
                     CNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKY
                     SQTRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTEN
                     QNEISLEKFNEFKETIQNKLFKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLP
                     YTNTNNVSTLIEQGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDVSKGDSWGKA
                     NYNDWSNKLTPNELADVNDYMRGGYTAINNYLISNGPVNNPNPELDSKITNIENALKR
                     EPIPTNLTVYRRSGPQEFGLTLTSPEYDFNKLENIDAFKSKWEGQALSYPNFISTSIG
                     SVNMSAFAKRKIVLRITIPKGSPGAYLSAIPGYAGEYEVLLNHGSKFKINKIDSYKDG
                     TITKLIVDATLIP"
     gene            complement(4586..5332)
                     /gene="cdtR"
                     /locus_tag="PC407_003303"
     CDS             complement(4586..5332)
                     /gene="cdtR"
                     /locus_tag="PC407_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HDF2498712.1"
                     /translation="MDILIFDNDVCFGIKLKEKINNILIKEGFDNDVIRLYYNANLLL
                     KELTEKNKVKIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYVKEKNYRSNFFTIYSDSSLWKISF
                     DEVIYFETSAIPHKIKLVTTSRIFEFYKSLRSLSDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT         1742 a          810 c          645 g         2254 t
ORIGIN      
        1 aaaaagaata tgttagaaga agagaatatt taaataaaaa ataccttata aagttaattt
       61 tttagtactt tataaggtaa atatatattg tattgtgggg acaaatttaa atcatttgat
      121 actaatcaac actaagaact aataactctc tatcgtctgg agtaattgca tatattctta
      181 atttcttgta tgtcataata ttttctccac ctgtaaaata actcctaaga ttaacataat
      241 tagttttagg ctgatttgga tctcctaaat aattacgcat ttctttatct ttagttccaa
      301 tatctttaaa tccagaatat tgtaaagtag cttcaactct atatttttgg atataatcga
      361 gagcttcttt attagtttgt gttggtgcaa aatacatacc atttatataa gtatttcctg
      421 tacttggatt aaaatttaaa tctgcaaaat atattttatg tgcatccatt atttcttggt
      481 cagttggaat tttaacttct ggttcatcaa gtatttcagg agtactattt agctctgtaa
      541 tagataagtt atctaagtat gttgtaccac taccaattaa tgttatctct atattagaag
      601 aatctttttc agtagtttca aattcataac taaattttgt atatccttgt tctggtacca
      661 aataatccgt tttctcttct tttgctttta tctttacaat tattgaatta gatgttaaag
      721 gatcctttga atatccacta aaaacataac gtttataagg ttttagctca gacataggga
      781 ttttaatctt cgtctcacca tttaatttat ttgctgagcc ttgtaaacca tcttgattcg
      841 tagtattgac attactccat gtactagggt aattattata atcatcaaaa ttagtaaagt
      901 aagttggtgt ttttataagt atattcattc ctctttcaag tttaacatta tatatctttt
      961 tatcagacaa agtttttaaa ctatctttaa tcttattggc tgtattatca tcaaatatga
     1021 gttcaacaca actttcatct attggtatat cattaaaata taacaaacca tcttttttag
     1081 tagcgccaaa agctttttca attgcttctc caattgtaag ttcaggtgtt ttatcttctg
     1141 gatcctgtaa atttttagca gtaactcttc tttcgtaaga ttcattctct gtatctaata
     1201 taatagatgc agaaatactg tcaatttgac ttatatagtc tgaccaacta tttccttctg
     1261 ttactatttg tccagaacta ttttttgtac caaaatttcc acttacttgt gttgtttcta
     1321 atttaatttg ctttccagca tctaattttt ttaattgatc ataatttata ggaatcagtc
     1381 tagagctaaa ttgatccatt gtgttaagag ctagaggtga aagccctttt ttgggataag
     1441 tatctccagg agatagatta ttgccaattt gattttcttg tgctttgata gttgataatg
     1501 tatctccatc taacactaaa tttgttgttg gtgtcacttt gtacataggt gcagtacctg
     1561 tgttgtaata tctaacattt gcatttatat atgcagattc tcctttgttt atacttaatc
     1621 cagtattcca tgattctcca ttactatctt gaacagcagt tgaattatct gttgtatggg
     1681 aataatttgt agttacatta gctgtgaatc cattttgata tcctacatta acagacacac
     1741 cagctgtatt agattcagtt ttactgttag tagtagctct ggaaacagtt ttaccttgat
     1801 cagtagaggc atgttcattt gtagatataa ttaatttttc catacctact ccaacaattg
     1861 gatatgctgc aactaacgga tctcttgctt cagtctttat agccttgtca aaagaacctg
     1921 aagctttttc ataatctgta tatggatctc cagcagtatt tgactctaaa taatttgata
     1981 catatttctt atagccttgt tctgcaaaac tatcttccca cttaactgca attaagtcct
     2041 taatagtata tccatttcgt tcatatgaat ctggtatatt atcattatct gtatccaaat
     2101 cttcgtcttc ccaatcagac atcaactttg ggtcaaagaa attgttattt gggataaatg
     2161 gatcatcttt ttctatatta gaataatctc ttaagaataa attttcttct ggtataattt
     2221 tcttcatacc atctaattcc caataaagat taggtgatga taaattatct attgaaccta
     2281 aatttttatc ttgtagctct attctaactt tatattcttt accctttttc atattaactt
     2341 taagtgtatt tgatatagta ctctcagtat ttacttgcat taagacatca tctctatcag
     2401 ttgataatgt atattcacca tccttagaag gaattattct tcctgtccat cgtatagatt
     2461 ttacatctga tttgtcttta tccagaagtt tatctacttt cttttcttca aattttaaat
     2521 taccatcttt tatgggtgcc attaatttta aatctttaaa gtgctcatct gtgaaataat
     2581 atcccattaa tccattgttt gggagtatat cttcatttac aatttctttt ttcttattag
     2641 aatgattact tgtactagtt tgagcatata caggatatac tagtgcttga ctaactatag
     2701 ctgtaagtgt taaaaaactt aatacctttt tattcctcat ttgtattttc attgtttctc
     2761 ctccttaaat ttgaaaaata ttaaattatt tatgtgaaaa aatattaagg tatcaatgtt
     2821 gcatcaacaa ttaattttgt tatagtacca tctttgtaag aatcaatttt attgatttta
     2881 aatttgcttc catgatttaa aagcacttca tattcacctg cataacctgg aatagctgat
     2941 agataagctc caggagaacc tttaggtata gttatacgta gtactatttt tcttttagca
     3001 aatgcactca tattcacact accaatacta gtactaataa agtttggata agacagtgct
     3061 tgtccttccc attttgattt aaaagcatct atattttcta gtttgttaaa atcatattca
     3121 ggggaagtaa gagttaaacc aaattcttga ggaccagatc ttctatatac agttaaatta
     3181 gttggaatag gttcacgttt taatgcattt tcaatgtttg tgattttaga atctaattct
     3241 gggttaggat tatttactgg accatttgat attaaataat tattaattgc agtatatcct
     3301 ccacgcatat aatcatttac atcagcaagt tcattaggtg ttaatttatt actccaatca
     3361 ttataatttg ctttacccca agaatccccc ttacttacat catctttaaa atcaagacta
     3421 cttacaacag atgcttctgc tttaatatag tgcttcccat ctataactat acgaacaatt
     3481 ttatctattt ttatactata tccttgctct attaatgtac ttacattgtt agtatttgta
     3541 tatggtaaca taccagtatt tctaggtaat tttaagtgca taagtaatgg tgtaggtttt
     3601 tcatcacctt ttccaggttc atataaagaa atatctttaa atccatcttg cttaaataat
     3661 ttgttttgta tagtttcttt aaactcatta aatttttcta atgaaatttc gttttgattt
     3721 tctgttctta ttactttatt aaatgcaaat ttttctggag attcaaaata atagacatac
     3781 ataggtttat ctattttatt ttttgatata gcatttcgaa gttctttata ttctttttct
     3841 cgagaatttg cttctatttg ataatcataa aaataatttc ttgtctgaga atatttactt
     3901 atttctacag aatctttttt atatgattct aatgcttctt tttcagatct ttcaagtttt
     3961 tgctctattc tttctgcttc ttttctttcc cattctttag ccttttcttt atctttaaga
     4021 aaatcttctg gtctttctat aggagccttg taagtagtat tgcaaacttt ttcactatag
     4081 ctttgtaagt cttgtgcata aatgttatta ggtaacaaac cacctaaagt tagatataat
     4141 atcaacaata tagatataca attactaatc cttttatgtt tcctaaattt tttcatttat
     4201 tctccctccc aatattagtt taattaactc ttgaacttta atttatttag attatataat
     4261 ctaaataaga attttttata taaatttcat aaaatatgta actatatatc ataaaaatat
     4321 gtatttatat gatatgtcag tacaaaataa caactgaata ttttaaagag actatttcat
     4381 acttttatga tttaatgagc tatcttgttg aatcataatc taggaacaat ggttgcaatg
     4441 tctaatagaa attgtatatg atatagtcat tatgtgtttt ttcacaaaac gattattata
     4501 tagaggattc ttaggaacta ctttttttgt ataaaaaaag agatggttca aattcttatc
     4561 ttgagacatc tcttttttct atttattatg ttttaataat gttctttaaa atatttctat
     4621 atgtattaga tatacgacat atatggccat tactcatttt aatattattt ttctttaggt
     4681 cgagagaaac aatatgatat ttattaacta caaaagattt atgtacacga ataaagcatg
     4741 catctaaatc tgataaagac cttaaacttt tatagaattc aaatattctt gaagtagtca
     4801 ctaattttat tttatgtgga atagcgcttg tttcaaaata tataacttca tcaaatgaaa
     4861 ttttccatag tgaggaatct gagtagatag taaaaaaatt agagcgataa tttttttctt
     4921 taacgtatct tttatgagca attttaatac aagcttttat tttactttca gcaatttcca
     4981 tatcatgttt taatataaag tccataactt ctaacctata atcgaagatc cctaaaatca
     5041 tttctatatg atttgtaaga aatatgattg gactaatata atctgattct ctaatttttt
     5101 gtgctatcca taatccatca cataattcat tacttatctt gtattttgca tctacaacta
     5161 taaaatatat ttttacttta tttttttcag taagttcttt taataacaaa tttgcattat
     5221 aatataatct tataacatcg ttatcaaaac cttcttttat taaaatatta tttatttttt
     5281 cttttagttt tattccaaaa caaacatcat tatcaaaaat taatatatcc actaaatacc
     5341 ctcctataaa aaattcaaaa gtaaaaaaac taaaagtatt ttataaaaaa tacgtaaaat
     5401 aacactagga gataaataaa attaaggata gttagaatta aggtatattt a
//
LOCUS       DAMJVX010000210         5428 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMJVX010000210 DAMJVX010000000
VERSION     DAMJVX010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.216"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(566..1972)
                     /locus_tag="PC407_003304"
     CDS             complement(566..1972)
                     /locus_tag="PC407_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2498713.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILKDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTIKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGVKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVAGIINAHRGKLDI
                     MSIDSQGLKTILKLPLIK"
     gene            complement(2017..2724)
                     /locus_tag="PC407_003305"
     CDS             complement(2017..2724)
                     /locus_tag="PC407_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2498714.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            2886..3809
                     /locus_tag="PC407_003306"
     CDS             2886..3809
                     /locus_tag="PC407_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498715.1"
                     /translation="MKHMEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGA
                     GKSTTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENM
                     TIMQRLLDLPKKNVNEALKIVRLENQKNKKVNQYSLGMKQRLGIAMAIMKFPKLLILD
                     EPTNGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQD
                     SIKELHNRGQSQIAIKAKNVVSAQKLLNTKGYMSLIQNGYLTIENLKDSDVAKINGML
                     VHSNIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3809..4570
                     /locus_tag="PC407_003307"
     CDS             3809..4570
                     /locus_tag="PC407_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2498716.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPILMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4582..5319
                     /locus_tag="PC407_003308"
     CDS             4582..5319
                     /locus_tag="PC407_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2498717.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1878 a          836 c          696 g         2018 t
ORIGIN      
        1 acataaatac atatatatat atgttatttc taaaaatact acacaaaaac tctactttag
       61 tagaactcaa tggtgcaaaa tacttcaatt taaaacattt atcgtacttt aaataaataa
      121 ttataataaa atatacttac aatagttaca tagaagctat tataactaca cagaacaacg
      181 tataattaaa acttttttag gatgtcatta tattttttct taaaattaaa aaacactata
      241 ccataattct attttagtag aatttttttg tcaataataa aattaaatat taactaaaaa
      301 ataaaaaaaa tggaattatc taaagaattt aataacctta caataattcc aaaatattat
      361 ttctatgaaa attttgatac catgagtaac tcagccataa gcgcataaag cacaagttac
      421 tctttacttt aataaaactt gacaagaaaa gaatttatcc ttattctact ctaatagagt
      481 tattttgtca ataaagaata aaattatttt caaaatattt tcattcttca ttcttcattc
      541 ttatctgtta ttattttata aaatatcact taattagagg taattttagt attgttttta
      601 gtccctgact atcaatactc ataatatcta atttacctct atgtgcatta ataattccag
      661 ctactatcaa aatacctaga ccatgatttt gacctttggc atatgcctca cttagtccat
      721 tttggctctt taataaacta ttaattcttt cttgactcat accttttcca tcatcttcaa
      781 caattatata gcaaaaatct aaatcagact tagaggtaac ccttatatta catccttgtt
      841 ggttatgagt tatactattt tgtattaaat tactaatagc cctttttaat aattttttat
      901 ctgcatctat aataatatct tcatttagta tatcaagttc aacataaaag tcattatcca
      961 aattattatt tataaactca gaaataagct ctctcaaaaa cgcagaaact ctaatcttat
     1021 cacaattcaa tgcttgcata ttatattcaa gtttagatac taaatttagg tcatttacta
     1081 attctctgat tttaacccct tgattgcata taatagacac ttgctcttgt tctctatttg
     1141 aaaggctact gctttcttca agttctccag catatccaat tatcattgaa agtggagttc
     1201 ttatatcatg agatattcct gcaatccagt tagacctagc ttcctctttc ttcctaagtt
     1261 tttcatcttt catttgcaac tctttagata cagtattaat agattttgaa atatcactta
     1321 gtattccttt ttcttgtaaa agtactggtt ctccttttga cataagcttt atgccagtaa
     1381 taattggttt tatagatttc accattttta aacctatgaa tagagctatt aaaaaagtaa
     1441 ctattgcatt aaccagaaac atacctatta ttaacatagg aaattgttct atttccttaa
     1501 tcgtataagt aaggttaaat ttagtgtatt tgtcctttgg ataacctgtt actaatattc
     1561 catttggatg ttcccataca tacacaggat agtcctttaa aaagaacctt gaaaactttg
     1621 ctacatctgt caaagtataa tgacttggaa tttcttttgg taaatcaaat ccccatattc
     1681 tatctccatt gttatcaatt aacattgccc aaactttatt tttatgtagt acttccttac
     1741 tagaatcatc taaaataaac tttccatctt ttaaaatcaa attatcttca atcataatca
     1801 agtttgattt tatttctctg ctatcacttg tatctaaatt atcagagtat ttagatacaa
     1861 atagcatata tgaaaacaga agaaaacctg ctatatttaa tacaataaaa agtaatgaaa
     1921 tcaagatact tgaacctata aatcgcctca atatgtactt tacactatcc aaaattaaat
     1981 cactcctctt gatttctttt attctaatat ctaaaattaa ttaatcatca acttatatcc
     2041 aagacctctt actgtaataa gatgtttagg tgtagatgga gttttttcta tcttctctct
     2101 aacccttcta acatgaacca taagggtatt ttcatatcca tacatatcat ctccccaaat
     2161 ggcttgacat agtgaatcat tagtaacaat tctcccttta ttttcataca actttaaaag
     2221 caacgaatgc tctttagctg ttaaagaaat ctcttgccca tctttctcaa cacatgcact
     2281 atttaaattc acaacaacag aatctcccag tttaaatact ggcaactcct cttcttctat
     2341 aggcacatat acacgattta atatacccac aagccttaaa gtaagttcct ttggtaaaaa
     2401 tggctttact atataatcgt cagcccctaa tcccaaacca atcaatctat cttcatcttc
     2461 acctcttgca gagagaaaaa taactggtat cttagatgtc tctctaattt ttctcataat
     2521 agagaaacca tctccatctg gcaatacaac atctaaaatt gcacaatctg gctttacatt
     2581 tctaaatacc ctaacagctt cttcataact tgatgctaaa aatactctac taaatccctc
     2641 ttttcttagg atttcatcta tcatttttaa taattccttg tggtcatcaa ccacaagtat
     2701 ttttttatct tttattgact ccataatcat caccctcctt tagtatacca tatacccatt
     2761 ttcacaactt tgcctaaata gacttttaag gttattgtaa ggtaaacttc agctaactga
     2821 aatgtaacca agttattata ctgatataag ttaaaagaat caataagcac agattaaaaa
     2881 aggggatgaa acatatggaa atgataatta gaacacaaag actttcaaag gaatacaata
     2941 aaatatttag agtaaaggat atagacctaa gagttccaca aggagctgta tatggttttt
     3001 tgggtcctaa tggagctgga aaatctacca ctcttaaaat gcttcttgga cttgctaaac
     3061 ccacacaagg aaatattaat attttaggta aagagttaaa tgaaaaaaat agaatatcaa
     3121 ttttaaaaga tataggttct ttgatagaat ctccatcata ctatggtcat cttacagggc
     3181 ttgaaaacat gaccataatg caaagactct tagatttacc taaaaaaaat gtaaatgaag
     3241 cattaaaaat tgtaaggtta gaaaatcaaa aaaataaaaa agttaatcaa tattctttag
     3301 gtatgaagca acgtcttggc attgctatgg caataatgaa gtttcccaaa ttattaatac
     3361 ttgatgaacc tacaaatgga ctcgacccag cagggattga ggaaattaga gaattaataa
     3421 aatctctacc agaaaaatac ggcatgactg ttctaatctc tagccatctt ttaagtgaaa
     3481 ttgaccaaat tgccacatca ataggaataa taaatcatgg cgaactggtt tttcaagata
     3541 gcataaaaga acttcataat agaggtcaaa gtcagattgc tatcaaagct aagaatgttg
     3601 tatctgcaca aaagctatta aatactaaag gatatatgtc acttatacaa aatgggtact
     3661 tgactattga aaatttaaaa gactcagatg tggctaaaat aaatggtatg cttgtccatt
     3721 caaatataga tgtatttaga atagaagaac ataaaaaaag cttggaagat atattcctag
     3781 aacttactgg taaggcggtg actttataat gaaatacctt ggtctagaat tttataaact
     3841 taaaagaaag aagattattc tgatgacctt tttatttgta gctgttgaaa taatgtggtg
     3901 tatcgtaaat tcaaataggg ctattacacg taatccagat atgttaggtg gatttgaata
     3961 ttcctatatg cttatgagtt ttgcaagttt aaatggtcta ttctttccta tcttaatatc
     4021 aataattaca tcacgcataa gcgatataga acataaagga gatacatgga agcttttaaa
     4081 atcttctgtg acatctttaa atagtattta tttatccaaa tttttatgtt ctgctatttt
     4141 ggtatctata cctatattga tgcaagtgtt atctattgtg ttgtttggac attttagagg
     4201 ggttattgag tcaatttctg tatctttact tttaaaatac acattaggaa caattcttgt
     4261 aagtattgct atcattgcac tacacctatg gattgcaact gtaattccaa atcaaatgat
     4321 tgcaattaca tttggcatga ttggtagttt tataggtttg acatcaggtt tgttttatca
     4381 aggtattcgt agattgttta tatggtctta ttacctagag ttatcacctt taagttatgc
     4441 ttatgataat gttttaggtt catctgtcta tagaattgat atgaattttt ctattacgat
     4501 tttagttttc ttaattggaa tgctaattta ttatattgga agaaatcatt tattaaaaaa
     4561 agaagtatag gagggattta aatgttaagg aagtcagttt tgggagaaat tctaaagtta
     4621 aaaaattctt ttatatggta tgtattatta gctttgccac ttataagtgt gttgattggt
     4681 tctggtaatt tttatctaaa tcaaggcata ctcaaaaaag aatggtacag cttatggact
     4741 caagttagtc ttttttatgg ggaatttttc tttcctatct taatcgcaat cttttgtgca
     4801 tatatttgta ggcttgaaca tatgaatcat aattggaata atgtaatgac actgccagtt
     4861 aatttaaaaa atatcttttt atctaaactt acagtagttt caatacttac agctattaca
     4921 caagttttct ttgttatctt ttatatagtt gcaggaaata tgtttggttt ttctggtagt
     4981 gtacctaaag aattgactgg ttggattgct aaaggttgga ttatttctat ttcaatagct
     5041 tctatacaat tatacatgtc tataaagatt agaagttttg caactcctat tggtattagt
     5101 ctttgccttt ctctatttgg tatgggatta atagtagcaa aacctgctgt tggtatattt
     5161 ttcccttatt ctatgttagg tagtgctatg ggggttataa agcaatctgc tttaaatgtt
     5221 aatgacacta tagttacaat atgtgttgca tttattacta ctatagtatt ttctgaaatt
     5281 gccaatagaa attttagaaa gaatgatgtt atttcttaat ggtaaatgct agatattttt
     5341 tggtttgaaa tttatattct ttttggtttg aaatttatat cccttttatt taaaaatata
     5401 tatcttttat ttgaatttta tatgtttt
//
LOCUS       DAMJVX010000211         5394 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMJVX010000211 DAMJVX010000000
VERSION     DAMJVX010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.217"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            94..474
                     /locus_tag="PC407_003309"
     CDS             94..474
                     /locus_tag="PC407_003309"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDF2498718.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            489..950
                     /locus_tag="PC407_003310"
     CDS             489..950
                     /locus_tag="PC407_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2498719.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            971..1561
                     /locus_tag="PC407_003311"
     CDS             971..1561
                     /locus_tag="PC407_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498720.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            1576..2733
                     /gene="alr"
                     /locus_tag="PC407_003312"
     CDS             1576..2733
                     /gene="alr"
                     /locus_tag="PC407_003312"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2498721.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            2766..3053
                     /locus_tag="PC407_003313"
     CDS             2766..3053
                     /locus_tag="PC407_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDF2498722.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            3022..3405
                     /locus_tag="PC407_003314"
     CDS             3022..3405
                     /locus_tag="PC407_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDF2498723.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            3576..4403
                     /locus_tag="PC407_003315"
     CDS             3576..4403
                     /locus_tag="PC407_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2498724.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            4396..5337
                     /locus_tag="PC407_003316"
     CDS             4396..5337
                     /locus_tag="PC407_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2498725.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGINAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
BASE COUNT         2152 a          571 c         1078 g         1593 t
ORIGIN      
        1 cataaactaa ataataatgt gtattatcga tagtaaattt ataaattcat tattaattat
       61 tgaaagaagt attataaatt ttctggagga acaatgaata tatttgatat tggcgtagat
      121 ataattgaaa ttgatagaat aagaaaggct gtagacaaaa ataataggtt tttggagaag
      181 atatttacag atagagaaat agagtatttt aattctaaga attttaaagc agaaagtata
      241 gcaggtaatt tcgcagccaa ggaagctata agtaaatcta taggtacagg aattagattg
      301 tttaatttta aagatataga agttctcaga aatgaaatgg gaaaaccaat agtaaaaact
      361 tataataatc tagcaaaaat gtgcatagat tataatgttt tagaaataaa agtttcaata
      421 tcacattcaa aagattatgc tatagcaaat gcgataacca taattaagga ctaaggagtg
      481 attacattat gatggataaa acagcaaaag aaataatgac aacagatgta atagtagcaa
      541 aacaagatga tagtatagca gatgtagcta atatgctaat tgcagaaaaa ataggaggtc
      601 tgccagttgt agattcagag aatagagttg ttggaataat ttctgaaaca gatattctta
      661 aaaaagaaaa atatatagaa gcacctctct atataaatct attacaagga ttgattttct
      721 tagatgactt aaaaaaagtt gaaaaagata taaaacaagt agctgcttat aaggttggag
      781 aacttatgtc taaagatata ataaaggttc atgaagatga taagtttgat gatgtagcta
      841 atgtaatgat taaaaaatca ataaacagag ttcctgtagt agatgatgac aacaaactta
      901 aaggtataat ttgtagatat gatataataa aagctttata caatgaataa actgaagtag
      961 gaggtgtgat gtgagaaaaa agtggaccat agtatgtatt atgttcttgg cactggttat
     1021 aattgtaata ggatgccaaa aacgacagtc cacaaaagaa gaagtataca aagattttca
     1081 aaagcagatt tctgatatga attattacag ttgtaaagct gaagtagaag tagttggaaa
     1141 taaaagccca cacaattatg ttttaattca tacttataaa aaaaccgata actataaatt
     1201 agaagtaatt tccccaaaac atttaaaagg gaaaagtata gaatatcaag gtgataaaat
     1261 attagttaaa aatccaaaga ttagtgatgt agtggagtta cctaatacag gaaaaaataa
     1321 tcaatatctt tttgtaggag actttataaa aaactatctt caaaatgagg agatgaaagt
     1381 aaagttatcc aaaggacatt tggttttaga gacttttatt cctggggata ataaatattt
     1441 taacaagcaa gtattatatg taaatgctga cacaaaaaat cctgaaaaaa tggaagtgtt
     1501 agataaagag ggagtgccaa gatttacagt aaaatacaaa gattttgaat acagaaacta
     1561 agggggacta aagacatgca aaaaataaca gtgcctacat gggcagagat aaatctagat
     1621 aacttaagat ttaacttaaa taatattaaa aatttattag aagaagatat taagatttgt
     1681 ggagtaataa aagctgatgc atatggacat ggtgcagtag aagttgcaaa attgctagaa
     1741 aaagaaaaag tagattactt agcagtagca agaactgctg aaggaattga acttagacaa
     1801 aatggcataa cacttcctat tttgaacttg ggatatactc cagacgaagc ttttgaagat
     1861 tctataaaaa ataaaataac tatgacagtt tattctttag aaacagcaca aaagataaat
     1921 gaaattgcaa aatctttagg agaaaaagcc tgtgttcatg ttaaaataga ctcagggatg
     1981 actagaatag gtttccaacc taatgaggag tcagtacagg aaataataga attaaataaa
     2041 ttagaatata tcgatttaga aggtatgttt actcattttg ctacagctga tgaagtaagt
     2101 aaagagtaca cttataaaca agctaataat tataaattta tgtctgataa attagatgag
     2161 gctggtgtaa aaatagctat aaaacatgta tcaaacagtg cagctattat ggattgccct
     2221 gatttaagat taaatatggt aagagcagga ataatattat atggtcatta tccatctgat
     2281 gatgtattta aagatagatt agaattaaga ccagccatga aattaaaatc aaaaatcgga
     2341 catataaaac aagttgaacc aggtgtagga ataagttatg gactaaaata cacaactaca
     2401 ggtaaagaaa caatagctac agttccaata ggatacgcag atggatttac tagaatccaa
     2461 aaaaatccaa aggttcttat taagggagaa gtgtttgatg tagttggtag aatatgtatg
     2521 gatcaaataa tggttagaat tgacaaagat atagacataa aagttggaga tgaggttata
     2581 ctatttggag aaggcgaagt tacagctgag cgtatagcta aagacttagg aactataaac
     2641 tatgaagtgt tatgtatgat atcaagaaga gttgaccgtg tttatatgga aaataatgag
     2701 cttgtacaaa taaacagtta tttgctaaaa taaaaccatg atattataat ctgggaggcg
     2761 attttgtgta cgataagagt aaagaaaaaa tagaagttac tttacctgac tctctgattt
     2821 ctgaagttga tagtatagtt cagatggaga acagtaacag agaagatttt gcaaaagctg
     2881 cttttcagtt ctatataact cagaaaaaga aaattcatat taaagaatct atgaaaacgg
     2941 gttatagaga aatgggtcaa attaatctgt cactagctga attaggtatg acagttgaag
     3001 aagtatcttc tgatgataaa aatgaaggga agattgccca aggtgagtac taattgtgaa
     3061 ataaagcgtg gtgatttata ttacgctgat ttaagtccag ttgttggttc agagcaggga
     3121 ggcgttagac ctgttctgat aatacaaaat gatgtaggta ataaatatag tcctacagtt
     3181 atagtatcag ctataacatc tcagattaat aaagccaaat tacctactca tgtagagata
     3241 agttccaatg aatatgggct taataaagac tcagtaattc ttttagagca aattagaact
     3301 atcgataaaa agagactgag ggaaaaaatt ggttgtctag atgaaaatat gatgataaaa
     3361 gtagatgatg gacttcaaat aagtttagga ttgtttactt tttaaagagg gatttgtctt
     3421 ttattagaca atcccttttt tcttttaaat atctcttaag gtttaatttt aatgtactat
     3481 gttaataaaa ttaaaaataa cgcaaaaaaa tagcataaaa gtgatataat aatatggaat
     3541 tgatataaaa ttacgaactc aagggaggta attgcatgag agaccataaa ggattaaatg
     3601 agattgcgcg tatcataaga agagatatag tttcaatgat acatagagcc aaatctggac
     3661 atccaggagg gtcattatca gtagttgaaa tacttactgc gttatacttt gatgaaatga
     3721 atgtggattc ttcaaatcct aaaatggagg atagagatag atttgtatta tcaaaaggac
     3781 atgctgcacc agcattatat gctacattgg ctgaaaaagg ctattttgat aaggaagaac
     3841 tgaatggatt aaggaagata ggaaggatgt tacaaggtca tccagacatg aaaggcacgc
     3901 caggtgtgga aatatctact ggttctttag gtcaaggttt ttcagtagca tgtggtatgg
     3961 ctatggcatc aaagttggat aatgctccgt ggaatgtgta tactttgctt ggtgatggtg
     4021 aggttcaaga aggaattgtt tgggaagctg ctatgagtgc tgcacattat aaacttgata
     4081 atttaattgc ttttttagat aataatggac ttcaaataga tggagatata gaaagcgtaa
     4141 tgagtttagg ttcaatagtt gataaattta aagcttttgg ttggaatgta attgagatag
     4201 atggacatga ttttgaccaa atatttgcgg ctcttgatat agctaaatca acagttaaaa
     4261 aacctacaat gatagttgca aaaactataa aaggcaaagg aatatctttt atggaaaatc
     4321 aagcaggatg gcatgggaca gctccaagtg atgaggagct agaaaaagcc ttgcttgaat
     4381 taggaggtgc tgacaatgag taaaatggcg acgagagaag catatggtaa agcattagta
     4441 aaattaggtc aaataaatga caatgttgta gtattagatg cagatttatc aaagtctaca
     4501 aaaacacatg atttttacaa gtcatttcca gatagatttt ttaatatggg tatagctgaa
     4561 caaaatttaa taggcgctgc ttgtggatta tcaactgctg gtaaaatacc ttttgcaagt
     4621 acttttgcta tgtttgctac gggaagggct tttgagatta taagaaattc agtttgttat
     4681 ccaaagctaa atgtaaaaat atgtgctact catgcaggtc ttactgttgg agaggatgga
     4741 gcatctcatg aaagtgtgga agatattgct ataatgagag ctataccaaa tatgacagtt
     4801 cttgttccag ctgatggagt tgaaacagaa aaaattattt ttgaaatagc taagtataat
     4861 ggacctgttt atgttagatt agggagaagt tcagtaccag ttttatttga tgaagattat
     4921 aaatttgaaa taggaaaagg tacagtctta agagaaggaa aggatgtatc aattatagct
     4981 tgtggaatta tggtaaatga agctctatta gcacaagaaa agttgcaaga agaaggtata
     5041 aatgctagag ttataaacat gtcctcgata aaacctatag acaaagattt aatattagaa
     5101 tcagcaaaag aaacgaatgc tattgtaact gtagaagaac atagcataat tgggggatta
     5161 ggttctgctg taagtgaggt agtaggagag tcttgtccaa ccattgtaaa gaaagttgga
     5221 attaaagata catttggaga atctggtact ccaaatgaat tgttaaaaaa atatgaatta
     5281 acttgtgatg acataataaa gactgttaaa gaagctatta ttgcaaaaag aatgtaagag
     5341 atataaatta ttcatgtttg ttaataatat aatatattta aaagagaggt ataa
//
LOCUS       DAMJVX010000212         5363 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMJVX010000212 DAMJVX010000000
VERSION     DAMJVX010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.218"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(234..2513)
                     /locus_tag="PC407_003317"
     CDS             complement(234..2513)
                     /locus_tag="PC407_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2498726.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITEKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYIARSNGNIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFAQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2784..4238)
                     /locus_tag="PC407_003318"
     CDS             complement(2784..4238)
                     /locus_tag="PC407_003318"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDF2498727.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYNSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4402..5271
                     /locus_tag="PC407_003319"
     CDS             4402..5271
                     /locus_tag="PC407_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2498728.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDATSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGYLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
BASE COUNT         1834 a          836 c          564 g         2129 t
ORIGIN      
        1 attactatta ttactatcta aagtattttg ctataatccc atatactctt acctcaaaaa
       61 cattcaaatg gaaaaaatac tacatattaa taaactttgc ccaataattc ctaaaataat
      121 atcctaacaa tgatttatgt ttttataata gtatattttt acataaaaaa gtgaacattg
      181 attaacaaag ttcacttttt tataaatttt tatgttataa ttcacaattt tatttaattt
      241 gtttcttttc tatttaaact atatctatct aatattaatt tttcatattc ttctattggc
      301 attggtctag caaataaaaa tccttgggcc atatcacaac ctataaacct taaaaactct
      361 gcttgctcta cagtttcaac accttctgaa acaactttta tatcaagttg ctttgccatt
      421 tgaacaatac ttgaaactac aatttgactc ttttttgcat ttcccactgt tataaagaat
      481 tgcctatcta atttcaatac atcaattggc atatctttaa gcatatttaa agaagaataa
      541 ccagaaccaa aatcatccat agaaatttta aatccaacag atttcaaagt ttgcattatt
      601 ttaaataata tttttgtatt gtcaaatatt gcactttctg tcaattcaat ctcaattaaa
      661 tttggttcaa tttcatattc ttttgttatt ctttcaagct cagaaataaa acctggattc
      721 tcaagatgta ctcttgataa gtttattgat ataggaaatg tggggaaatt ttcattttcc
      781 caacgtttaa aatttttaca aacttgggca aatacaaaca tatccaattc tgttataaaa
      841 ccatttttct caaaaatagg aataaataca cctggactta ttagaccttt tttgggatgt
      901 tgccaacgta caagcgcttc agcacctaca attttagtag ttgataaatc aaccttaggc
      961 tgtaaataaa ctttaaattg ttcatttttt aaagcatcat gcatttcttg ttcaatatca
     1021 cgttcttcta tgagagtttc ttttaaattt tcagcataaa tctcataaac ttttaaatat
     1081 tttcttttta ctgtagtttt tgcaatcata gctttgtcaa gacaggtaga aatatttgtt
     1141 tctccctctt caacaaaata tacacctatg gatggaatta aattgagttc tactcccaaa
     1201 cttgttttta tcattgacaa ttttttacgt ataagttcac atatttctat aattgactct
     1261 ctattatttt tcttttcaaa tattatagca aaattgtcat ttgaaattct tgaaaataca
     1321 gattgctctt taaatatatt cttaattatc tctgctattt cctttaatac tctatctcct
     1381 tcttcatacc caaacctatc atttactagc ttaaacttat ctatatcaaa ataaattaaa
     1441 actagattct ttttctcatg agaggataaa aattttcctc cttcaagatt aaatttattt
     1501 gaattgccaa tacctgtaac gttatcttta aaagcaaatt cttccaattt ttccttactc
     1561 tttttatgta taaatagtat ataaattgtt aatgccgtaa agattataat caacactaca
     1621 catgctgata atgtaagttt tataatagcg tatgatttct cagatatagc agttttagga
     1681 acttcagaaa aaagatacca atcattgatt cctattgggg cataacttaa ataatatcct
     1741 tttccaaaca tactataact taatgaacct gatttaaagt tttttatatt tgacttcata
     1801 ttattgatac tagattttga tattgaatca cttctgccaa tgtaagaaaa taaattttct
     1861 ttagtagagt tggcgttttt gctgcttgga tttaaaacaa tattaccatt acttctagct
     1921 atataagaat atccttttcc attaaatgta ggtacagaaa gtattttttc atacaacttc
     1981 gtactatgtg tggcaaatat aactccttta acctcattat ctttataaat tggcacagaa
     2041 tatacattaa tattactatt ggtattttta tcatcttcta ttacatcttt taatttttct
     2101 gttataacca cttctccttg cattgatttt aagaaataat ctcttttaga gaagtcttct
     2161 atataaccat ctgtagtgta tgctgtacca tttggtaaaa taactcccat tcttttaaat
     2221 gaactattaa tagcttgtct ttttagtata gaaaggatat tatctacttc aaaagactct
     2281 tctccctcaa taaatattga aattgactct agtaacttta tatctccatt aatttgtttt
     2341 tttaacacat tgacgctttg atttgctatt tctgaaagat attgattcgt ttcttcacct
     2401 aatgtcttat tcaaccttga accataaatc aaacaggaaa ttgtaattac taagatactt
     2461 atgacagcaa ttatggacaa accataccta ttattttttg catcacttaa cattatgctt
     2521 cctgcctttc tcgaatctat gtatgtataa tcattatctt atgacaatta ttgtaaacat
     2581 tttataataa tacttatttt tccatattta ctgtaattat acaaaattat aaactttatt
     2641 acaacaataa attaattaat atagatatta gggtttatat tattgcatta tctattttac
     2701 aatcatttac atataaaaaa atgtaatatc aaagtgaaac ttgatattac atttttgttc
     2761 ttgtattttg tttaattttt atgctacatt ttacttaaac actcatttgc aacttcttta
     2821 tcatcaaaat gatgcttaac tttacctata atttgataag tttcatgtcc cttacctgtt
     2881 ataattacaa catcatcctt ttttgccata cttatagctt ctgctatagc ttgttctcta
     2941 tcaacaacaa catggtaatt ttcttttttc ttgtcaattc cttcaagtat atcttttatt
     3001 attgcctctg gttcttcagt tcttggatta tcagaagtta taatacataa atcagaatat
     3061 ttttgtccaa ttgctcccat caaaggtctc ttttcagtat ctctatctcc accacatcca
     3121 aacacagaaa ttatttttcc ttctgcaaat tgttgagttg tctttagtac attttcaagt
     3181 gcatcaggag tatgagcata gtctacaatt acacttatac ctttatcatt tggaactgtt
     3241 tcaaaccttc ctgatactcc attagaaagt ctaagacctt ctttatatat tggtttaggt
     3301 atgccaagta catagcaagc agctataact gctaaagtat tatatactgt aaacattcct
     3361 ggaactggta taaatattac ttcttcatat gaaggtgtta ttaatctata agatactcca
     3421 tccgaatctg attttatatc tctagccata aaatcagcct tagtatcaac accataagtg
     3481 taacaaggta catttatacc ttttatattt tcatatatct tttttccacc ttcatcatct
     3541 atgtttatta tatttgccat agtagtctta aaaaataatt tttcttttgc ttttctataa
     3601 tcctctaaat ctttatgaaa atctaaatgg tctggtgtta aatttgtaaa tatgcctagt
     3661 ttaaagtctg tttcatcaac tctatttaat gccaatgaat gtgatgaaac ttccatcgca
     3721 cagtagtcac atccattatc caacatatta ttaaaatgat attgtaaatc tatactttct
     3781 ggagttgttg aattggaaac aatgtctttt tctccgtcaa aaatttttat agttcctata
     3841 agtccaactt tattcttatt taaagttaat atctcattta gtatagtagt tatactagtt
     3901 tttccattag tacctgtaac tcctataacg ttaaatttct gagatggatg attataaaaa
     3961 ttatcagcta cttttgccat atcttttctt gtatctttta cctttataaa tgtacatcct
     4021 tttatttcta catcttcttc aacaagaaat gcacttgctc ccttttctat ggcatctttt
     4081 atatacttat gaccatctga cacaaatcct tttatacata taaataatgt accttttgta
     4141 acctttcttg aattatattg tacattatta atatctatat ttaattcacc cttgacattg
     4201 attatatcca acccctgtat aatatcattt aatttcataa atgctttcct ccctttaaac
     4261 tctttctgct aaaattacac tacatataat attatattaa gaatttttta ctatgtgaat
     4321 ggttaaaaat gcttgaaata ttgcaaaaaa tgctctataa ttttctataa tataactgag
     4381 gaaaggtctt ctataaaaac tatggataat aaaatcgggt acttaaacga taactttaaa
     4441 atatttaata ttagagataa aaaagatata caatttgaat accataatca tgattttaat
     4501 aagataatta tatttataaa tggaaatgta acttattttg ttgatggtat tccctataaa
     4561 ctaaagcctt gggatatact ctttatcagc aataatgaaa tccataaacc agttatagat
     4621 tcaaatgtct tttatgagag aattgcaata tggattagcc cagaatttat gaaaaaaaat
     4681 agtgatgcta ctagtgactt agaaaaatgt tttaaaatag ctacggagaa taaatgcaat
     4741 agattgcgtt taaatataca tgatattgaa tctattaaga gttttattga gcaaataaat
     4801 atttccgaat ccaatactga atttggaaat gaaatattga gaaatgcatt atttatacaa
     4861 cttatgatat tcttaaacag aattttccta aaaaatgaaa atatggaatc aacagaagac
     4921 gttttttatg atgaaactat taagaaagta ttaaactata taaattcaaa tatagatgat
     4981 aagctttcaa tagatagcat atcgtctgaa ttttttataa gtaagtatta tcttatgaga
     5041 aaatttaaat ctcatactgg aacgtctata cacaattata tagttcaaaa aagattaatt
     5101 ttagcaaagt cactaataac caatggttac cttatgagtg aagtttgtaa taaatgtgga
     5161 ttcaacgatt attctagttt tgtaagggca tttaaaaaaa actacggtgt atctcctaaa
     5221 aattatataa acaataaaaa tcctttagat attactcttt tagatgaata gataatttat
     5281 gtaaatttta ttttaataaa aacttgaagt attagtgtat ttatcttact aatacctcaa
     5341 gttttttaat gttttaaaat ata
//
LOCUS       DAMJVX010000213         5347 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMJVX010000213 DAMJVX010000000
VERSION     DAMJVX010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.219"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(204..1388)
                     /locus_tag="PC407_003320"
     CDS             complement(204..1388)
                     /locus_tag="PC407_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2498729.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="PC407_003321"
     CDS             1761..4112
                     /locus_tag="PC407_003321"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HDF2498730.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERFEVQGNKKMKNFPFLMGQGVWKGSDDLVPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..4673
                     /gene="nrdG"
                     /locus_tag="PC407_003322"
     CDS             4134..4673
                     /gene="nrdG"
                     /locus_tag="PC407_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDF2498731.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2089 a          593 c          850 g         1815 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttactt tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttattatgcc attattttat atacaaattc ttttaatttg
      241 tctaatccag ataaaaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttct tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa tcatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcgt ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtattggga ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caagattgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagaacat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttgaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaatac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actatttcct aattttagtt
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaggcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagatg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgct ttgaggttca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgattta gtaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga ggcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatac ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aagagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaaa
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa
     4681 ataaacagga ttaaatatat gcatatattt aatcctgttt attttcatat aatatttcaa
     4741 gtttgtataa tgtaacaatt aaaccaaaag actcattgta tatttgaaat taagtttttt
     4801 atagtttata ggtgttttat gtggattacg aatgatatag tatcgataca ttatttaatc
     4861 gattcttatt ccaagaaaca attaatttat agaaattctt aattgttaca atataaatat
     4921 ctagataagt tataagatac tgataaataa tttataaagt ttattaatct aatattgtgt
     4981 gtgaatgtta agtggtcaag tagtctaaaa aatgaaaaag atttaaaaaa gtgttttaac
     5041 taaacacata agtgttaaat tcatgtataa gtataggtta gatacataag aaataattat
     5101 gtaatatcta tctttgtaaa aaaatattaa attttgtaat ttgtggataa atatgtttat
     5161 aatgtggata atatttaatg taataagtta aagcaatatg aatcaactgt ttataatgtg
     5221 gataatatta taaatgtatg aataattgca gtatttaaaa acaaaaaaag ttaaaaaagt
     5281 tgttgactct aaaagaaaaa gttggtatag taatacttgt cctcaagaaa agggcaaaca
     5341 aataaag
//
LOCUS       DAMJVX010000214         5306 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMJVX010000214 DAMJVX010000000
VERSION     DAMJVX010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.220"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            99..935
                     /locus_tag="PC407_003323"
     CDS             99..935
                     /locus_tag="PC407_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2498732.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFKFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1288..2865
                     /locus_tag="PC407_003324"
     CDS             1288..2865
                     /locus_tag="PC407_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2498733.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2858..4042
                     /locus_tag="PC407_003325"
     CDS             2858..4042
                     /locus_tag="PC407_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2498734.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     TL"
     gene            4060..4659
                     /locus_tag="PC407_003326"
     CDS             4060..4659
                     /locus_tag="PC407_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2498735.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4683..5153
                     /locus_tag="PC407_003327"
     CDS             4683..5153
                     /locus_tag="PC407_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2498736.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIELKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         1983 a          561 c         1020 g         1742 t
ORIGIN      
        1 aaaatatata cgaaagtaaa attttaaata ttgacattaa attaaatata ctatactatg
       61 taagtgtgat gagagaattt ttttagaggg gatttatagt gaatataaaa aagatattta
      121 ataacaatgt agtagtaagc tccctagaag atggcacaga aataattgtc actggtgctg
      181 gagtaggatt taagaaaaaa gtaggcgatt tgatagatga aaatttgatt tctaagaaat
      241 attttgttca agacgatcaa agggacaaat acaatcaaat acttaataag acctctattg
      301 aatactttaa aatttcagaa gaaattatag aaaaggctaa tgaagtttta aatactcaag
      361 tgaatgattc aataatatta gccttaacta gtcacataga atttgctgtc caaagagaaa
      421 aacaaggtat aaaattacca aacttaatat tgaatgagac aaaacaactt tatagggaag
      481 aatttaaatt tggattatgg gcaattgatg aaattgaaaa aaggatagga ataaaattgc
      541 ctgaagatga agcaggatat attgctattc acataatcaa tggtttggaa aattctcaaa
      601 aagatgaagg tattaatata ctagagtttt caaaacaagt tattcagata attgaagaag
      661 tacatggatt taaattggat gtaaattcat taaattatac tagacttata actcacttaa
      721 agttttttgt tcaaaggatt ctccgaaaag aaacttacaa tgatagtgat attgaagata
      781 tgtataagtt agtgaataaa aattacaata aacctaaagt gtgtactgaa gaaattgcag
      841 cattggtatt agacagattt gaatataaga ttagtaaaga agaagaatta tatttaatga
      901 ttcacataaa taaaataaca aacagtggtt gttaggattg taactgataa agcaggcaaa
      961 acctaagtca atgagcttaa ctattagatt aagttttttg atttaggttt ttttattaga
     1021 tacaatcaaa atacagacag tgacttttag gattgtaact ggttaagcag gcaaaaccta
     1081 aatcattgaa tttaactatt aggttaagtt ttttgattta ggttttttta ttagatacaa
     1141 tcaaatacag acagtgactt ttaggattgt aactggttga gcaggcaaaa cctaaatcat
     1201 taaacttaat tattaaatta agttttttga tttaggtttt tctataaatt tgattatgtt
     1261 tttagataaa aataggaggg gaaaattatg aaaaaaaata agttaggttt atggaacttc
     1321 ttccaaatgt tagggaaaac gtttatgttc ccaatagcac tacttagtgt ttctgggatg
     1381 atgctaggac ttggggcagg ttttacagac cctaagatga ttgaaatggt tccatttcta
     1441 gggaatgaat atgttaactt agttttaaac ttcatgttaa caataggatt atttgcattt
     1501 aacaacttgg gagcattatt tgcaatggca attccactag gattattaaa aaaagaaaaa
     1561 gagtttggtg ctttttcagg attagtagga tttatagcaa tgcatatagg tacaaatttt
     1621 tatttaacaa gagctgattt gttagtacca gcagaacaga tgtcaactaa tggtcaagct
     1681 atgattatgg gtatacaaac atataataca agtgttttag gtggtattgt agcaggatta
     1741 attgtttatg caatatatga taaagttagt aatttaaaga taccagaatc attaggattc
     1801 tacagtggac caagattagt tccaattgtt agtttaatag ttatgagtat agttggatta
     1861 ttaataccta ttgtttggcc accatttttc aatgcattcc aaagtttagg aagatggatt
     1921 tcttcagcag gaccattagg atatttttca tatgcagtgg cagaaagagt tacaattcca
     1981 tttggactaa atcatcttgt aacatctgta ttccgtttta caccaatagg tggaactgct
     2041 gttattgatg gagaaacgta ctttggtaca ttgaacatgt ttatggcata tgtagaaaat
     2101 aatcagatta tacctcttga tttagctggt aaaatggaac aaggtaaatt gatgattcaa
     2161 tatggattag ctggtgctgc attggctatc taccgtacag caaaaccaga aaatagaaag
     2221 gcacttaaag gacttttaat atcaggagtg ttgacagtta ttataggtgg tataagtgaa
     2281 ccaatagagt ttctattctt attcatatca ccagcattat ttgctttcca tacatttatg
     2341 aatggtctag caaacatggt acttccatac ttaggcgtgt tgatgggatt cacaggagat
     2401 ttaattgcat ttataagttt tggtgtgtta agaggaacag ctacaggatg gccaatagca
     2461 gtattagtag cagttttata ttttgcaata tactattttg tatttaagtg ggcaattatt
     2521 aagtttaata taaagacccc aggacgtgag gataaagaaa taaaagtatc tgaaggaaat
     2581 ggttcagatt tcaaaaattt gtctacatat aaaggacaac aaatgatatc tgctttaggt
     2641 ggtcaatcta atatattatc actagataat tgtgtaacta gattgcgttt aaaactacaa
     2701 gatgtatcat taataaatga ggatgcaatt aaagaggctg gtggtatagc agttgttaaa
     2761 ttggatgagc atacaataca agtaattata ggaactcaag tgtattcttt aagaaagcag
     2821 atggataaag ctatggaagg tcatttggag gcatgtaatg attaattttg ataaaaaagt
     2881 agaccgattg ggtacatatt gtacccagtg ggactatgta gaagatagat ttggtaagaa
     2941 ggatttgctt ccttttacaa tttcagatat ggatttagag tttccatttg aaataacaga
     3001 ggtgttgcag gaaaggttaa atcatagagt tttgggatac agtagatgga aacatgatga
     3061 ttttagaaat tcagttacga attggtatat taaaagattt aattgtagcg ttgatagtaa
     3121 ctggatatat tatagtccaa gtgtaatgta ttcaattagc aaacttattg agatgttaag
     3181 taatgaagga gatggaatcc ttataaatac tcctgcatac aatgcttttt atgatgtgat
     3241 aacaaataac aatcgtaata ttataaagtc acatcttgta aataggaatg gattctattc
     3301 aattgatttt aatgattttg agaaaaagtg taaaaaatct aagatattta ttttatgtaa
     3361 tcctcacaat ccaactggta gagtatggag tgagttagaa cttgttaaga tgataggaat
     3421 ttgtaagaat aatgatgtca agataatatc agatgatata cacatggata ttgtatatga
     3481 caatagaatg acacctatct taaatgttgc aaaggactat ttagattctg tattcatttg
     3541 tacatcagcg tctaaatctt tcaatatacc agcattgact gggtcatatg tgattatacc
     3601 aaatgaaggt attagaaata aatttgaaaa tatcactaga taccgtgatt ttgtaaattc
     3661 gccagcaatt ctagcaatat tagctacaat ggtctgttat gatgactgcg aatattggtt
     3721 agatgaacta ttaaaatacc ttaaagagaa tcttaaatat acgattgatt atataaaaga
     3781 taatttagaa ccattgaaat tggtcatgcc agaaggctgt tactttgctt ggatagattt
     3841 ttccgaactt aaaataagtg gtgagaaatt ccaaaaatta ttgatggata ttggagaagt
     3901 tgctattatg tcggggtctg tatatggtga agaaggtgag ttttatttaa ggttaaatgt
     3961 ggcatgttca agagaaaaac ttgaagatgg tttaaatagg attaaaaaaa ctatggatta
     4021 tattgcaaaa agaactttat agtaggaagg gagatagtta tgggatattt aaaaacaatg
     4081 gttgatgtta tgactactga aatgaatgcc ataagatgtt taatagatga agcaggaata
     4141 gaatatgaaa gtatagttag tgaaattgca aattgtgaag gcaaagtaat ttttatgggt
     4201 gtaggaaaat ctgcacatat aggtaagaaa ttggcagcta catttgctag tactggtaca
     4261 cctagctttt ttgtacatgc aacagaggca gtgcatggag atttaggaat gattgaaagt
     4321 aaggatatta caattttaat atcaaatagt ggaaattcta tggaagtggt aaactgtata
     4381 aaatatatta aagcaatagg ttctaagaca attgcattta cttcaaacag aaattcagta
     4441 ctggcaaaag agtgtgatta tgctttaata tatcctgcaa aggatgaagc agaccattta
     4501 aatctagcac caacaacatc atctacaatt actttggttt taggagattc tattgcatgt
     4561 gcattatcta agagtagtaa ttttggttct tcagattttt ataagtacca tccaggagga
     4621 agtttaggtg agaagttaaa aacagctaac aatggataaa agcaaaaagg agagtagttt
     4681 ttatgttcaa aatgtttaaa aaaaaatcaa taaaatcacc catatcagga aaagtaatag
     4741 agttatctaa aatacaagat ggtgtatttt ctcaaaaaat tatgggagag ggtgtagcta
     4801 tagattctac aggagacatt gtatatgctc caatcaatgg aaagatagta gtagtagcag
     4861 agactaaaca tgcatttgta atagaattag aaaatgggat ggaacttctt attcatgttg
     4921 gtttagatac tgtaaattta aaaggcgaag gttttgaggc attggtgagt atgggagaac
     4981 aagtgaagga aggaacacca atgttgaaaa ttaaccgtcc tttgattgaa ttaaagggta
     5041 tatcacttat tactccagtt acaataacta atcattcaga gtacaatatg aatacatgta
     5101 atgtaggcaa tagcgttgaa ggtgggaaag atactgtaat tgagtttaaa taaagaatta
     5161 aaatacaagg ttaatattta taaataccta taaacttatg ttttataaga ttgattgtat
     5221 ggatttattg tataaaaaag aactctttaa ttataaatta aggagttttt ttattttgta
     5281 aatattgttt agatttatat agaaaa
//
LOCUS       DAMJVX010000215         5268 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMJVX010000215 DAMJVX010000000
VERSION     DAMJVX010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.221"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            292..2304
                     /locus_tag="PC407_003328"
     CDS             292..2304
                     /locus_tag="PC407_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2498737.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNALIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIDDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
     gene            2673..3893
                     /locus_tag="PC407_003329"
     CDS             2673..3893
                     /locus_tag="PC407_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2498738.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISSTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            3930..>5268
                     /locus_tag="PC407_003330"
     CDS             3930..>5268
                     /locus_tag="PC407_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2498739.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYSLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWFIRIISWSTL
                     AVGFATALGSFWPESATEYKGCIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFVIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     IALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISRE"
BASE COUNT         2149 a          587 c          814 g         1718 t
ORIGIN      
        1 tatttacaaa attatattat caatagaatt tattaaaata aatttttttg ggtgtatatt
       61 ataatttaat cgaatagaaa tgaaaaatat acatacagtt taagttttaa gcaacaatta
      121 gggggatttg gtgacaagat ttttaattat aagaaagaaa gtgtcttaat gcaactttta
      181 taaagtcatt atagacactt tcttttttat ttgataaatt taaattatca aaattacatt
      241 gctttataca actattttgt tggaaactat ttattatata aggagaaata aatgaaaaaa
      301 tcagtagctc tggtaaatga tagtagaaaa gatttaatag actttttaga aaataatttg
      361 aaattagttt ttggagattc tataaatatt aatagatact tcataaatga aataaatgat
      421 aatgatatta taaatgatga tgtgatatta gtaatgtcag tagaaagatt agacaaaatt
      481 ataaataata tactagataa gaaaaaagtg attgtggtaa gaagaacttt tagggaagat
      541 aaaatttata atctactatc tttaccacag ggtacaaatg ctctaatcgt aaatgattct
      601 gatgaaacta ctctagaaac tataagttta ttttataaaa ttggagtgac taatattaga
      661 ccaattccat atatgaatga taataactat aaaaatataa aaatagctat aactccagga
      721 gttccagaaa aagtacctag ctttatctca gatatatttg atttaggtca cagatatata
      781 gatatttcta catttataga aataataaat ttattacaaa ttgattcaaa ggaaatacaa
      841 tctaatctag ttaaatactc agaagaaata ataagtttag atactggtat aaaagataaa
      901 tataaggaat tatttttaaa aatagaagaa ttagatacaa tattgaattt atcaaaagat
      961 ggtatattat ttacttcaaa agatggtgaa ataaatacat ataatagtaa agtaaaagat
     1021 attttagaca taaatgagga tatatatgga aaatacattg atgatatatt tgtagatagt
     1081 ttaaaagttt tattgagtga gaaagagata ctagataaag ttgtcgtatt caataagaaa
     1141 tatataaatg taaataaaaa aaacatatac aatagggatg aaaaaatggg cacatattat
     1201 agtttacaag aaataacata cataaaaaaa ttagaacaaa atctcactaa aaaattaaga
     1261 gaaaaaggac aaatagcaaa atatacattt aaggatataa aaacaaatag tcctaaaatg
     1321 tttgaatgta tagatttagc taagaaggtt tctaaatctg acttaagcat acttatcaga
     1381 ggtgaaagtg gaactggtaa agaactcata gctcagtcta tacataacaa ctcaaataga
     1441 aaaaatcaac cttttatagc agtaaattgt gcagccgttc ctgaaaattt attagaaagc
     1501 cagctattcg gatatgacaa gggaactttt actggaggtc taaaagatgg aaaacaagga
     1561 ttatttgaac ttgctaataa tggaacaata ttcttagatg aaataggcga tatgccttta
     1621 gaattacaaa caaaactact tagagttctt caagagaagc agataatgcc tgttgggtct
     1681 cacaatgtaa ttaatataga tgttagaatc atatctgcta caaataagaa tttagaacaa
     1741 atgattgata atagccaatt tagagaggat ttatattatc gattaaatac aattccaata
     1801 aatatacctc ctctaagaga aagaaaagaa gacatattaa ttattatgga agatttaatt
     1861 aataagaaat tagtaatcac accagaagca aaaaaactta tacaaaacta tatgtggaaa
     1921 ggtaatataa gagaactcca aaatgttaca tcctatttaa acattatgtg tgaagatatt
     1981 gtattagaga aagatttacc tccaaactta aggtcttcag ataacaaaaa tacatcatta
     2041 aaactaaaat atagtaagaa tgatatattg aatatattag agatactaat tttaaataag
     2101 gaatcagatg ttggtattgg taggggatta attttaaaag cactactcga taagaacctt
     2161 caaattacag aaggaaaaat taaaaaaata tttgaatatc taaaaaaaga agaacttata
     2221 atttgtagtt caggaagata tggaagtaaa ataactcaaa aaggagaaga tttttacaat
     2281 aaattaaaat ataaaggtct ataaaattat aatttatcaa acaaagaaat ctattcagga
     2341 ttaaagtact ccttcaatag gtttttttat ttttcaagta ccctaaaatt ataatttatt
     2401 ttgtacataa ataataggaa ttaataggaa taggataatt tttttatttc ctattttttt
     2461 tatataaact agcatataaa atatattttc ttcaaaataa tattttttac tgaaaattga
     2521 aactatgcaa tcatttttta tactatacat atagataaat gattatattt attcaatatt
     2581 cttataaaaa acaaaattct ccatcgaaaa ttatggcata ataattgctt attatattaa
     2641 taaaacaata aaaagcaatt ggaggaataa aaatgcaaca ggctttgaaa ttaaaatatc
     2701 aaaccaaaga attaggaaaa gattttgtaa tagaaagttg taattctata aaaccatggc
     2761 ttataaacat aagaagagaa ttacataaaa ttccagaatt agctcttgaa gaaaatctta
     2821 ctaaacagaa ggtaatttct tatttaaaag aaatcggaat tgattatatg gaatttacaa
     2881 aacacaatgg aattatggca tatattttaa aagaaagtgc agataaaacg atttgtataa
     2941 gagcagatat ggatgctctt cctattgaag aagaaaacaa cataccttat aaatcaatac
     3001 attctggaaa aatgcatgct tgtggtcacg atgcacatac aaccatgcta cttggagcat
     3061 gtaaagtttt acattcaata aaagataaac ttaatgtaaa tgtaaaattc ttatttcaac
     3121 cagcagaaga aggttttgga ggagcaaaat ttttagttga ggatgggtgt ttagaaaacc
     3181 ccaaggccga ttatatattc ggacttcatg ttatgccaca tatagaaaca ggccttatcg
     3241 aaacaaaata tgatacatta aatgctagtg tagacactat aaaaataagt atcaaaggta
     3301 aaagagctca tggtgcatat cctgaaaatg gtattgatgc aatagttaca gcttcacaaa
     3361 ttgttacatc attacaaact ataataagta gaaacttaga accaaataat gctgccgttt
     3421 tgactatagg taaaatatat ggtggtgatg cacacaatgt aatatgtgaa gatgtaaaac
     3481 tagaaggcac tttaagaacc ttaaatagca aaacaagaaa ctttatgatt gataaaatag
     3541 ctaaaattgt tggagacact gcatctgcat ttggttgtgt tgggacttta catgtaagtg
     3601 atgaaaacta tcctgctgtt ataaatgaaa aagaattagt agatacagtt atatcaagta
     3661 ctaaagaatt acttggagag gaaaaattta tattgagacc aaacccgtct ttaggtggtg
     3721 aagacttttc tttttacaca gaacattgta aaggagcatt cttccattta ggatgtaaaa
     3781 atgaggaaaa gggcttaatt tcacctcttc atacctctag cttcaatata gatgaagatt
     3841 gtttaccaat aggtgttatg atgcatgtaa tgaatacttt gtattttaat tagtacagta
     3901 attaataaaa gaataggaga taaattaata tggaaaaagc taagaaatta ggtctattta
     3961 gtatgatatt attagggatt aatagcataa taggttcagg tatattctta ctaccaggta
     4021 aagtttatag tctagcaggt caaaacagta tgtttatata catttttgct acactcttag
     4081 ttctatcaat acttttatgt tttgcagaag ttggaagcat gtttgataag aatggaggag
     4141 catatctata ctccaaaaaa gcttttggag attttatagg atttgaagtt ggtacaatgt
     4201 cttggtttat tagaattata tcatggtcta ctctagcagt aggatttgca acagctttag
     4261 gttcattttg gccagaatct gccactgaat acaaaggttg tatagctgca atactcgtaa
     4321 cactactttc aataaatagc ttatttggaa ttaaaagcac aaagataatg aataatgtaa
     4381 taactatagc taaattagta ccattaatag tttttgtgat agttggaata ttctttataa
     4441 aatttgtaaa tattgttcct tcaggaaatg tagttaactc tagtatgggt ccagccatta
     4501 tattagtatt ttacgcattt accggctttg aatcatttat tgtggcaagt ggagaaatgg
     4561 aaaatcctaa aaaaaatctt ccaatcgcat taataaccac aatatttata tgtgcaataa
     4621 tttatatatt aattcaaatt gtctgtatgg gtatacttgg agatagacta tttgaaaaca
     4681 gtattccaat tgcagatgct tcaagtgtat ttttaggcaa ttatggtaaa gttttcatat
     4741 ctgtagcaac attaatatct atatttggta taaatatagg ttcatcaata gtaactccta
     4801 aatgtggttc ttctctagca gaagaaggtt ctcttcctgc atttatagga aaaacaaata
     4861 aatatggtgc tccatatgta gcaataataa tatctttaat ttgttgtata ccacttgtgc
     4921 taactggaag ttttgaacaa cttgcagtaa tgagtgtaat tgcaagattt gctcagtaca
     4981 taccaacttg tttatcagta atagttttaa gaaaaagaac agatgttaag gcctctttta
     5041 agattccatt tggtcctgtt ataccaatgg tagctatttt aggtagctta tggttgttac
     5101 aacaagcttg ggcagaagat atttcaaaac ctataactca aaatagagtc ttaataggat
     5161 taggggcaat gttgttaata gctcctctat atatatttat gaaaaaaaat aaggaaatcg
     5221 agcataaaaa taaatcagaa gattctaaat tatatatttc gagagaaa
//
LOCUS       DAMJVX010000216         5147 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMJVX010000216 DAMJVX010000000
VERSION     DAMJVX010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.222"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..519
                     /locus_tag="PC407_003331"
     CDS             <1..519
                     /locus_tag="PC407_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDF2498740.1"
                     /translation="MNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFPVGRLDKDTEG
                     LLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLDDGYKTMPSQL
                     NILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLDESLELGEYRE
                     LTDEEVKLIEER"
     gene            546..1907
                     /gene="rlmD"
                     /locus_tag="PC407_003332"
     CDS             546..1907
                     /gene="rlmD"
                     /locus_tag="PC407_003332"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2498741.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMNEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            2430..3464
                     /locus_tag="PC407_003333"
     CDS             2430..3464
                     /locus_tag="PC407_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HDF2498742.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPYEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKNKKRKKI"
     gene            3551..3754
                     /locus_tag="PC407_003334"
     CDS             3551..3754
                     /locus_tag="PC407_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HDF2498743.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            3925..5085
                     /locus_tag="PC407_003335"
     CDS             3925..5085
                     /locus_tag="PC407_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HDF2498744.1"
                     /translation="MKLSKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVIIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
BASE COUNT         2038 a          508 c         1016 g         1585 t
ORIGIN      
        1 atgaataaac ctgatggata tatatcagca actacagata aatatgaccc aacagtatta
       61 gatttaattg atttatcata cttagctttt gaaccatttc ctgttggaag gttagataaa
      121 gacacagagg gattattagt tttaactaat gatggaaagc tttctcatag agttttatct
      181 cctaagaagc atgtgcctaa gacttattat gcaaaaattg atggagtagt tactgaagaa
      241 gatgtagaag cttttttaga aggtgtagtg cttgatgacg gatataaaac tatgccatca
      301 cagttaaata tattaaaaag tgatgatgaa tcagaaatag agcttacaat acatgaaggt
      361 aagtttcacc aagtaaaaag aatgtttgaa agcgtaggca aaaaagtagt atacttaaaa
      421 agattgtcta tgggcaattt aaaattggat gaaagtttag agttaggtga gtatagagag
      481 ttaacagatg aagaagtaaa attgattgaa gaaagataat aaagatacta tagtaaattg
      541 gaggaatgtc tgtgaagaaa aaagatgtta tagaatttga agtagataaa atggaatttg
      601 gtggtacatc tttaagtcaa gttggagata gagttatcca tatgaagggt ggaataagtg
      661 gtcaaaaggt gagagctgga gttaaaaaag ttagaagtaa gaaagctgaa gttaagatga
      721 tagaattatt agagaactca ccacttgaaa ctgaagaaac ttgcaagcac tttagagagt
      781 gtggaggatg tacactattg tctgtgccat atgaaaaaca attagaaata aaagaaaaac
      841 aagttatgga tttattttta aagcaagatt tatttggatt tcagttttta gggatagaag
      901 gaagtccaga aaacaaatat tatagaaata agatggaata tacttttgga gatgaagtaa
      961 aaaatggacc tttgacatta ggtcttcata aaaaaggaaa gcatatagat atacaaactg
     1021 ttgaggagtg tatgttggtt gatgaagact tctcaaagat attagtttct agtgtagagt
     1081 ttttcaatga aaaaaagctt ccttattata gaacaatgaa tcacaaagga tatttgagac
     1141 atcttgtagt tagaaaaggg attcatacta atgagattat ggttaatata gtaacttctt
     1201 ctcaagaaga ttttgatatg aatgagttta aagatatgct tttagggatt gatttaaagg
     1261 ctgagttagt aggtgtactt catactatta atgatggttt agcagatgca gtccaatgtg
     1321 atgagttaag agtattgtat ggaagagatt atatacaaga agaaatttta ggtcttaagt
     1381 ttaagatttc accattttcg tttttccaaa ccaatactaa gggagctgag gttctttata
     1441 gtattgctag ggattttatt ggagattaca atgataaggt tgtatttgac ttgtatagtg
     1501 gtactgggtc tattggacag gttatggctg gtgctgctaa gaaagtgtat ggtattgaga
     1561 tagttgagga agctgttgtt gctgctaatg agaatgctaa gttgaatgga cttactaatt
     1621 gtgagtttat tgctggggat gtggctaagg ttgttaagga tttaaaggac aagcctgatt
     1681 tgattattgt tgaccctcct agaccaggca ttcataagga tgctattagg gatatttgtg
     1741 gatttggtgc taaggagatt gtttatattt cttgtaatcc taagtctttg gttgttgatt
     1801 tggttgattt taaggggtat ggttatgaga ttaagatggt taagtgtatg gatatgttcc
     1861 caaatacgcc acattgtgag actgtagtta aacttattag agaataacag ggtttcagac
     1921 ttttatattg tgtcttaaaa gtagatttgc cacctatttg ccaccataat tatttttggg
     1981 tggcaaattt attctacaat gatttcaaaa atatttacca tctggtttct catgttatta
     2041 gttacatgta aatatgtatc catagttgtt gaagttttag catgtccgag tatatgctgg
     2101 atatctttta tattagtacc attttctaat aacatagtat cagggttttt aaaataaagt
     2161 gtgtattctt ctagagtagt gataattaaa atatcaaata tcactataca aaggaaagtg
     2221 cacccttttt atgtttagca aaaacgaaat attaaaaaaa cttattgaag aatatgatgt
     2281 caaaacaact acagatattc aagatatgct aaaagattta tttacaaata caattcatca
     2341 aatatttcca ttgtcgaatt ttaacggttt aaacataata tattgactga tataataaaa
     2401 tcatagaata ttttaggggg aaatagatta tgtttaagaa aaagttaata gctgttgcaa
     2461 tgagtataac tatgattagt gtagggagct tttcgtatgc tcattcagga agaactgatt
     2521 catcaggagg tcatcgtgat aataaaaata aatctggatt aggaagttat cactatcatt
     2581 gtggtggtta tccagcccat ttacataata atggtggttg tccttatact ggaggaggaa
     2641 gcagttctgg aacaacaact tcggttaata atgaagaaaa gcaaaagagg tctgttggag
     2701 aaaaaggata taatcaaggg tatgaagatg ggtataaagg aaattatagt tcatcaaatt
     2761 atagtggaga ttatagtgat acatatgaat caaagtactc agaaggatat gaaaaaggta
     2821 aggctaaatt agaagaggaa gaaaaggtgg ctaaagagac tggatataat ttagggctta
     2881 caggtgctaa atctaataat acatatgaaa aagaagtttt aaaaaatgct tatgataccg
     2941 gatattcaac tggatacaat gaatataaga ctaaaaagat agaagaatat aaagctaaag
     3001 ggatagagga tagcaataaa gataaagaaa aaatgacatt tgaagaaaac atagattctg
     3061 aatttataga tgcttataat aatgcttatg acgaaataca agaacaatta aaaaatgatt
     3121 acactactca aggatttgag agtgctatca aaggagaaag atttgacaca tcaactatag
     3181 gtaatgttaa atatgctaat tggtttaaag aaggatatga taatggtaag gttaagttac
     3241 ctaaagcaaa agaaagtgta tataatcaag gttacaatga agaagatttt agtataccat
     3301 atgaaatgaa gtctattgaa actaagttaa aaggtgtata tgatgaaggt ttagaaaaaa
     3361 gagaagaaga gaaaagtaga aatgctactt atggggttgg agctggaaca gcagctgtag
     3421 ttgctggagt agtatataaa aataaaaaac gtaaaaagat atagatgcta gatataccaa
     3481 tgatgacaag tgtataacaa atataatttt aagtataaat atttaaaata tagaagggaa
     3541 ggttatatct atgggattta actttaggaa aagcataaat ctaggaggag gactcaaatt
     3601 aaatttaggt aagaagagtg taggtataag tgctggtgtt aaaggtgcta gagtgtcagt
     3661 taattcaaaa ggaagaaaat cagctacatt gagtatacct ggaactggaa tatcatatac
     3721 aaaaactagt acaaagaaac gtaaacctaa ataaatattt gatatactaa gaaatagact
     3781 gttacaaata gtctattttt tagtatatag atttaaaaag tgacaaaatt ttgaaatata
     3841 aactaatttc ataaaggaaa atattctttt atgtagaatt ataatattta agtaatatta
     3901 taaaatttat aggaggattt cattatgaaa ttaagcaaaa cactattggc aatagggatg
     3961 tctgtttcat tattgattgc tagttgtcca acatcaaacg ctcttagttc tattaattca
     4021 ataaaaggta caaataagta tgaaacagca ggtttaatag cagataaaca aaattatgat
     4081 attgcaatac ttattaattc tgaaaatgga ttatctgatg gattgtcagc aagtggattg
     4141 tcaggagcta aaaatgcccc aatattgtta actaagaaag atacaatccc ttcagaaaca
     4201 ttaaaaagat tagaaaaggt aaaagaagta tatctaattg gaggaaaaaa ttcgatagga
     4261 gcatcagttc agaaaatatt aagtgataaa aaaataagca taaatagaat aagtggaact
     4321 gatagaatag atacaagtca taaagttgca aaagaaatag gaagagtaag tacggttaaa
     4381 aaagtaatat taactaatgc ttttaaaggc gaaccagatg ctatgagtgt tgcttctata
     4441 gcagttagag ataaatctcc aatagtatta actgatggaa atactacaag ttttgctaca
     4501 gatggattag aaacttatgc aattggtgga aaagtcagta tgagtgataa gttaataaat
     4561 gaaactaaag caacaagaat aggtggagta gatagatacg atacaaataa acaaataata
     4621 aataaatttt ataatggctc aaaagaattt tacatagcaa gtgggactga tttagtttat
     4681 tctttagttg gttctacaat tgcacaaaaa acacctatag tgttagttgg aaatggaagt
     4741 aataagagta tattaaaaaa tgcaagtaaa ataactgcta ttggaaatat tgatgatgtt
     4801 attataaaac aatgtttaaa tataactaat aatgttgggg attcaaatac aggtattgta
     4861 aaagatacta attcaaataa tacaaacaaa ccaagtaatg agcctagctc taataatcct
     4921 ggattaactg ataaaagtac agtatatgta actcctaatg gaaaatcata tcatctaaca
     4981 aaaaattgtt ctacactaaa aagaagtaaa acaataaata aagtaacatt aaaacaagct
     5041 aagtcacaag gtaaaataga cccttgtaat gtttgtgtaa aataatgtat tagccaaata
     5101 tatattaaaa aagacaatta attaagattg tcttttttat tgaagaa
//
LOCUS       DAMJVX010000217         5130 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMJVX010000217 DAMJVX010000000
VERSION     DAMJVX010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.223"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            141..1034
                     /locus_tag="PC407_003336"
     CDS             141..1034
                     /locus_tag="PC407_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2498745.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIAEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEV"
     gene            1082..3382
                     /locus_tag="PC407_003337"
     CDS             1082..3382
                     /locus_tag="PC407_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498746.1"
                     /translation="MKKIFSIFISCMVVVTTITGCSSGEVIKNTSPINSVKNLDFSFE
                     VKPEIFEVSVESGGSKEKISEPSKERKVSNFKKNKTEASWTYPDENIDINIKKEDSYL
                     DVNIKSNLEKETSFNWPKVEGESYILPLYEGKFIPSNDKYWKEYLNESTYNVIESFSM
                     QFFAVNKAKYSVIYIIENEFNNVIEFSSKDKINFTFKHEYPSINKNKEYGFKIYLTNK
                     NITDVAKTYKNYIVDKGEFKTLKEKSKENKNIEKLYGAPHIYFGDKSIITINDIEWNK
                     LKENLSDDLTNWMKELLSMKSGNSEFIKVINQIKTQDYIDKYQKNQIVQAFNEVMQMN
                     TFYNEKVFESKDREIKKVLDKGISKLSRVELIEFNKKLLKSKLEESVNDVATWAKYNT
                     TDVINDINKSGIKNAWIGFDDMEEGYISLEMVKRANELGYLVGPYDSYHSIHKPGEEK
                     WSTASFKDKSLYENATIENKKGEKETGFQGEGRKLNPTLSLPSVKERVQSILDEGYEF
                     NSWFIDCDATGEIYDDYSQNHITTQTQDLNARLERMSYIRDHANMVIGSEGGNDFSSS
                     TIAFAHGIETPAFAWVDKDMSKNKGSEYYVGKYYSPTGGVPEIFSKQIPVKERYKKIF
                     IDPIYSLPLFRLVYNDSVISSHQWLWGTLKIKDEVGSRMMKEVLYNTPPMYYIDRTEW
                     EKHKDEIISNNKVWSAFNKKAIKKEMTDFKFISEDRLVQMTEYGEDLKVLVNFSQKDI
                     KYNDEVIKANSLVIYDADSKVIYKPE"
     gene            3616..4107
                     /locus_tag="PC407_003338"
     CDS             3616..4107
                     /locus_tag="PC407_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HDF2498747.1"
                     /translation="MYENNKVIYNFERCEKLENALYLSQFNTYKNFKLTSKKTVINSL
                     DTSTYNMNIHSISLVDTMECISSEDEQMTGKKLILVGDIDTDVTLDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            4135..5082
                     /locus_tag="PC407_003339"
     CDS             4135..5082
                     /locus_tag="PC407_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498748.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNSIVFGCIEVEIDYSCKNR
                     SNFKTVNKFNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
BASE COUNT         2202 a          469 c          835 g         1624 t
ORIGIN      
        1 ctatatatta taacaatagt agtgccttta attaaatata tttcataaat cttcataaaa
       61 tcttcaaaat ttcgtaaaga atatgtcaca aaagtaaggt atattatatt cagcagatag
      121 tataagggag gacaatattt atgaagaata aaaaattcat tgttataata ctgataatat
      181 caattttaat aggtgtatta gtgaaggaat attcatctag agagataaaa aaggatgata
      241 taaatgtcag taagtatata aaatatgctg atttagcaag taaaaataat gcacaagtta
      301 actggaagta tgtagcttcc attgttgctg tgttaaataa aaataactta aaaaacgtta
      361 aggattcaca gatacaagaa gtatcagatt tatttgttaa gaatttttct aaaaataata
      421 aaataaataa gttaagtgat attttggatg agttagaatt tagtaataga caaaaaagat
      481 tggttgataa ttatatagac aatcttaagg attatggtat aaaaccagaa agattgaagt
      541 ctgatacaaa atatatgaag tttattgctg aaattaaaac agaagcaata caaaattata
      601 aggattataa aatacttcca tctataacta ttgcacaagc tataatagaa tctagctggg
      661 gcaagtcaac tttggcaaaa caatataaca atctatttgg tataaaggct gacgcttatt
      721 ggaaaggtaa gtcagtgact ttagaaacga aagaacattt agatacaatt atagatgata
      781 aatttagaat atatgatgat aaaaatgaat ctataaaaga ccatgcaaaa tttttagcca
      841 caaataaaag gtataaaaac aatggtgtat ttgatgcaaa aacgtatata tatcaagcta
      901 aagcattaga aaaggcaggt tatagtactg cgaaggatga aaatggcaat agtatatatg
      961 cagctcgtct aatagaatta attcaacagt ataatcttca attaatagac agcgaaattc
     1021 aaagtgaagt gtaactttta tgtaacatag gagtgatata tttatcgagg aggtggtttt
     1081 tatgaaaaaa atattctcga tatttataag ttgtatggtt gttgtaacta ctattactgg
     1141 atgtagtagt ggtgaggtga taaaaaatac atcacctatc aatagtgtta aaaatttgga
     1201 ttttagtttt gaggtgaaac ctgaaatatt tgaggtatca gttgaatctg gtggttcaaa
     1261 agaaaagata tctgaaccct caaaagaaag gaaagtatca aattttaaaa agaataagac
     1321 agaggcatcg tggacgtatc ctgatgaaaa tatagacata aatataaaaa aagaggacag
     1381 ctatttagat gtaaatatta aatctaattt agaaaaagaa actagtttta attggcccaa
     1441 agttgaaggg gaaagttata tactaccact ttatgagggt aagtttatac caagtaatga
     1501 taagtattgg aaggagtatc taaatgagtc aacttataat gttatagaat ctttttcaat
     1561 gcaattcttt gcagtaaaca aggcaaagta ttctgtgatt tatattatcg aaaatgaatt
     1621 taacaatgta attgaattta gttctaaaga caagattaat tttacattta aacatgaata
     1681 tccaagtatc aataaaaata aggagtatgg gtttaaaata tatttgacta ataagaatat
     1741 tacagatgta gcaaaaactt ataaaaatta tatagttgat aaaggagaat ttaaaacatt
     1801 aaaagaaaaa tctaaggaaa ataaaaatat tgaaaaactt tatggagcac cacatattta
     1861 ttttggagat aagtctatca tcacaataaa tgatattgaa tggaataaat taaaagaaaa
     1921 tttatcagat gatttgacta attggatgaa agagctttta agtatgaagt ctggcaatag
     1981 tgaatttata aaagtaatta atcaaataaa aactcaagat tatatagata aatatcaaaa
     2041 aaatcaaata gtacaagcat ttaatgaagt tatgcagatg aatacttttt ataatgaaaa
     2101 agtttttgaa agcaaagata gagaaataaa aaaagtatta gataaaggta tatctaaatt
     2161 aagtagggta gaacttatag agtttaataa gaaattattg aaatcaaaat tggaagagag
     2221 cgtgaatgat gttgctacat gggcaaaata taatacaact gatgtaatta atgatattaa
     2281 taagtctggt attaaaaatg cgtggatagg atttgatgat atggaggaag gttatatatc
     2341 cttagagatg gttaaaagag ctaatgagtt aggttatctc gtagggccat atgattcata
     2401 ccactctata cacaaaccag gggaagaaaa gtggagtaca gcatcattta aagataaatc
     2461 tctatatgaa aatgctacca tagaaaataa aaaaggtgaa aaagaaactg gatttcaagg
     2521 agaaggaaga aaactaaatc ctacattgtc tttaccaagt gtaaaagaga gagtacaaag
     2581 tatattggat gaaggatatg aatttaattc ttggtttata gattgtgatg ctacaggaga
     2641 aatctatgat gattattctc aaaatcatat aacaacacaa acacaagatt taaatgctag
     2701 acttgaaaga atgtcatata taagagacca tgcaaatatg gtcataggtt cagaaggtgg
     2761 aaatgatttt tcaagtagta ctatagcatt tgcacatggt attgagacac cagcatttgc
     2821 ttgggtagat aaagatatga gtaagaataa aggcagtgaa tattatgttg gtaaatacta
     2881 ttctccaaca ggaggagtac cagaaatatt ttcaaagcag atacctgtaa aagaaaggta
     2941 caaaaaaata tttatagacc caatttactc tctaccttta tttagattag tttataatga
     3001 ttctgtgata tcttcccatc agtggttatg gggaactttg aagattaagg atgaagttgg
     3061 aagtagaatg atgaaagaag tattatataa tacaccacct atgtattata tagatagaac
     3121 tgaatgggaa aaacataaag atgaaataat ctcaaacaat aaagtatggt cagcgtttaa
     3181 taagaaagct attaaaaaag aaatgacaga ctttaaattt atttctgagg atagactagt
     3241 tcaaatgact gaatatggag aagatttaaa ggtattagtt aatttttctc aaaaagacat
     3301 taagtacaat gatgaagtca taaaagcaaa ttctttggta atatatgatg ctgatagtaa
     3361 agtcatatat aaaccggaat aatagattta atgaaaataa agattgttta aatttcaagt
     3421 aaaaaataat ataatattca tttagtttgt attaaagtag tgactatatt aatctggata
     3481 attagtatag tcactattat ttatattctt tcctaaaagt aagtagtaac tgaatcgtga
     3541 ataatcaaaa atggtaattt gaaatagttt ttatgaatag tattaatata tagatatagg
     3601 gagattgatg tgattatgta tgaaaataat aaggtaatat ataattttga aagatgtgaa
     3661 aagctagaaa atgctcttta tttatctcaa tttaatacat ataaaaactt taagttaact
     3721 tctaaaaaaa ctgtaataaa ctcattagat acatctactt ataatatgaa tattcatagc
     3781 ataagtctcg ttgatactat ggaatgtata tcgtcagaag atgaacaaat gacaggaaag
     3841 aagttgattt tagttggcga tatagatact gatgtaacat tagattatgc aggcaataaa
     3901 agaaatagaa attctagtaa aaaaaataca tttaaattaa aaataccctt tagtacattt
     3961 atacaaatgc ctagaaagat agaaaataaa gataaaatta atttaaagta tcttatacaa
     4021 gatattactt catctatttt agatgataat ttatttatta gtgttactgc aattataagt
     4081 tatgaaaata gttccaagat agagtaatct ttaaattata gaaaggtgga tgcaatgaga
     4141 ataacgaaaa ctacttttaa tgatgtattt tcaaatgatg caaatggaag tattataaaa
     4201 gtagaaaaat atgttgataa aaaatgtgca tctgtatttg agattttgac ctacactatt
     4261 attgttacaa atacaagtag gtacaaaact ggaaatatat ttttcaaaga ttatatttct
     4321 aaatatatag agtttataaa taatactgta aaagttaatg gcattataaa acgtggatta
     4381 gacccacagc aaggttttta catagggaga atagatgcaa gttgtaaaaa aataatatct
     4441 tttaagagtg tagttttacc aaattctgca cataggatta taaagaatag tgctaatata
     4501 tactattatt ataagtgtaa tctagataaa ttcccaacaa gaatatcaat agaaagtaat
     4561 agagtttata ctaatgtaaa taaaattgta ttcaaacaat tgaatataag taatacttta
     4621 aaagctccta aaaatctaag agatatatta aaagtaaatg taaatagtaa aatatttgat
     4681 gttaaaccta tgaaaaatct tataaacaaa ggtagtaatt tgaaattatg taactcgata
     4741 gtttttgggt gtatagaagt tgaaatagac tatagttgta aaaataggag taattttaaa
     4801 acagtaaata agtttaatga tattaaaaaa gataagtatt ataaaaatga tatacaaaat
     4861 aacaatgaaa atataaacga ttgtaaaaag caaataagtt ttggtgataa taaggtaaaa
     4921 aaaataacaa aaacttttgg attttcttgt ttcatttgtt ctccagttgg aattatttat
     4981 gaagatatga aaaatataaa tataaaaatt gaacatacat caataaatga attaaatcca
     5041 gaagaattat ttgtaaatac atcactttta ttgtattatt aagtatttta aagctaaaac
     5101 ttaaacttat taaaatagca tttggttaaa
//
LOCUS       DAMJVX010000218         5110 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMJVX010000218 DAMJVX010000000
VERSION     DAMJVX010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.224"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            403..1194
                     /locus_tag="PC407_003340"
     CDS             403..1194
                     /locus_tag="PC407_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HDF2498749.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRSVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            1223..2443
                     /locus_tag="PC407_003341"
     CDS             1223..2443
                     /locus_tag="PC407_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDF2498750.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVDTMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            2463..3158
                     /gene="pxpB"
                     /locus_tag="PC407_003342"
     CDS             2463..3158
                     /gene="pxpB"
                     /locus_tag="PC407_003342"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDF2498751.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            3188..4174
                     /locus_tag="PC407_003343"
     CDS             3188..4174
                     /locus_tag="PC407_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HDF2498752.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRSKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLNSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(4393..4959)
                     /locus_tag="PC407_003344"
     CDS             complement(4393..4959)
                     /locus_tag="PC407_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2498753.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
BASE COUNT         1961 a          579 c          934 g         1636 t
ORIGIN      
        1 aaaatataaa gatattttac ttacaaagaa tgataattat atagatgaaa gaaacaaata
       61 aatgaagaaa aatgattctg tcttgcatat aaagtgatta aaaatgaaaa atactttaaa
      121 aatgaaggtt aaggccctta ttttaagtga aaataattaa ttattaacta aaaaatggta
      181 aaaagatttt ttgaaaaaga aatacaaatg aatgttcaat taaaataatt gcaaaaatat
      241 tccttaaatg gtaaaattat attaattaaa tgcaatagaa tagttgcaaa aataaaattg
      301 aatacaagtg ataaaatatt tcatgcaata tataaaataa acaaaattta aaaattaaaa
      361 ctacttattc tatcaaaatg ttagcaagga gggggtcata aattgtataa agtagattta
      421 aatagtgatt taggagaaag ttttggaacg tataagattg gtttagatga agaagtttta
      481 aaatatattt catcagctaa tatagcttgt gggtttcatg caggtgaccc tagtcatatg
      541 gaaaaaacag tacagctagc taaaaaaaat ggagtgaaaa taggcgcaca tccaggtttc
      601 ctagatttaa taggatttgg aagacgtgag atgaaaataa caaagcagga agctaaagat
      661 tatacaaaat atcaattggg tgctttaatg gcatttgcaa gttcaaatgg atgtaatata
      721 caacatgtaa aaccacatgg agcattatac aatatggcag ctaaagataa agagttagct
      781 atggggattt gtgaagctat atatgaagtc gataaagata ttatactgtt ggggctatat
      841 aatagtgaaa tgataaattc tgctaaagaa attggactta ggtttgcaaa tgaagtattt
      901 gcagatagag cttatgataa taatggtttt ttggttccaa gaagtgtaga aggagcagtt
      961 atacatgata caaaacatgc aatagatagg gttgttagga tggtaaaaga aggaactgta
     1021 gaaactttaa caggtgaagt tatacatatc aaagcagatt ctatatgtgt acatggggac
     1081 aatccaaaag caatagagtt tgtcaaagaa ataagaaagc gttttgaatt agaaggtata
     1141 gaagtttgtc cattagaaaa catagaagtt tgttcgtcag agaacatagt ataagactga
     1201 taacatgagg gggagataaa atatgagtac aaaaaatatt acagataaaa ctaaaaataa
     1261 aaaagatgtc ggagcactta taggggctgc atttataatg gcaacatcag caattggacc
     1321 agggttttta actcagactg cacaatttac ccaagatttt ggaccaaatt tttcatttgt
     1381 aattttaatt acaacaatcc tttttatagg ggctcaagta aatgtatgga gagtaattgg
     1441 tgtatcagga cttagaggtc aggatatagc aaataaaata ataccgggat taggttattt
     1501 agtagctttt ttagtagctc ttggaggatt agcttttaat ataggaaatg ttggtggagc
     1561 tgcacttggt atgaatgtaa tgtttaatat gaatatgaca ttaggaactg tattaagtgg
     1621 attaatagct atatttgtat ttatgtcaaa aaattcaaat tctctagtcg ataagataac
     1681 taaattttta gcattaggaa tgataattat agttggatat gtagcaatat caaatcatcc
     1741 tcctgttgga gaggcagtat caagaatggt aaaaccagaa aatccaaaag gattgatatt
     1801 cccaataata actctacttg gaggaagtgt tggaggttat ataacttttg ctggaggaca
     1861 tagattaata gacggtggaa taacaggaga agaaaatata aaagaaataa ctaaatcttc
     1921 tcttttgggt atattagttg acacaatgat gagagttctt ttatttttag caatactggc
     1981 agtggtttca aaaggtcttc aacttgaccc agaaaatcca gctgcttcag cttttaaatt
     2041 tagtgcagga gcaataggat ataaattctt tggacttgta ttatggtctg ctgcaattac
     2101 atcagttata ggagctgctt atacatcagt ttctttctta aagacactaa atccttttat
     2161 agataaatat gaaaaatatt ttataatagc atttatagca atatcaacac ttataatggc
     2221 atttatagga aaaccagcta ctttattaat tttagcagga gctttaaatg gattgatact
     2281 tccaatcaca ttaggaatta tgcttatagc atctaaaaga aaagatattg ttggagatta
     2341 caagcatcct acatggcttt taatatttgg attaatagta gttttaatat ctgcatatac
     2401 aggaataact tctcttagca gcctaggtgc tttatttgct taatttagaa gaggtgacat
     2461 ttatggaaac tagatacttg atatcagggg ataaggcagt ggttgctgaa tttggaaatg
     2521 agatatcaga agatataaac aaaaaagtaa tttcatttat gagagcaata gaaatatcga
     2581 atcttaaagg tattgtaaca gaaatggtac caacatatag gtcacttatg ataagttata
     2641 atccattaga aatagatttt gatagtttga ttgagaattt aaaaaaaata gaagataatt
     2701 tggaaagtat agtattacca aaacctaaaa tacatgagat tccagtatgt tatgatgagg
     2761 tttttggtat agatattaaa aacgttgcaa gctacaataa tctcactgta gatgaagtta
     2821 taaaaataca cacaagcaga gagtacttga tatatatgct agggtttact cctggatttc
     2881 catatctagg tggtatggat gaaagaatag ctacacctag attagaagtg ccaaggacta
     2941 aaatttatgg tgggagtgtt ggaatagcag gttcccagac tggtgtatat cctattgata
     3001 gtcctggtgg atggcaaata attggtagga cacctttaaa attatatgat gaaaatagag
     3061 aagaacagat tttgcttaga gctggagact ttataaaatt tgttccaata acattagatg
     3121 aatttataga aatcgaaaaa aataacttaa gtatttaaat atcgttatag tacagggagt
     3181 ggtatatatg ggattcaaag ttgaattagg aggtttccaa acattaatac aagacagagg
     3241 tagagttggc tatggtcaat atggtgtatc tggatgtgga gcaatggatg agtatgcaca
     3301 tagagtagga aatatattag taggaaattc tgaagatgag gcttctttag aagttttaat
     3361 gttaggtcct acaattacct ttgatgaata tacgcaaata gcagtaactg ggggagattt
     3421 aggggctaaa ataaatggaa aagaaattca aaactggagg tcatatcaaa taaatccagg
     3481 tgatgtatta tcatttagag gtgtgaaatc aggtgctaga gcctatgtgt caatagctgg
     3541 tggaatagat gttccacttg caatgggaag taagtcaact tatacaagat ctaaaattgg
     3601 cggatttgaa ggtagagcac ttaaaaaagg agattatata aatactttta ttcaggaaaa
     3661 agattttaca ataaataaaa aattaaattc aaaatatatt ccaacttata gttcagaaat
     3721 cgtactaaga attgtaaaag gtcctcaatt tgatgctttt tctaacggtg aagtagagaa
     3781 atttttatca aataaataca aagttacgaa tgaaatagat agaatgggtt gtagactaga
     3841 tggagagagt ataaagcacc ttaatggagc agatattata tctgatggta tttcttatgg
     3901 agctatacaa gtacctggtc atggaaaacc gattataatg ttatcagata gacagactag
     3961 tggtggctat actaagatag ggaacgttat aagtgtagat ttgtataagt tagcacaagc
     4021 aaagccaaat gatgtagtaa aatttgaact tgtagatata tatgaagcac atagactatt
     4081 gagagagcaa gaagataaga ttcaagatat ctacaagagt atgaaaaata taagagttgt
     4141 aaaggcaaaa gtattaaatg atatagcagt ttaatttaat tttaacaaat acaattttta
     4201 ttataattta taatataact tacaaatagc tatttactat ttaccataat taagatggtt
     4261 tagattagtc aaatagctat ttttttagtt tgagtaaata ttttaaattt aaataaatac
     4321 ttcaatttta aaataaaagc aatatttaaa tttattatat tatctacaaa aagtaggtgt
     4381 gttatgttga gtttatttag attttattgg ccaaattgga ggtgatataa ctccatatcc
     4441 acgagctatt gtatctttac ttggattgat gattttatgt ggtacattag cttttataaa
     4501 tgttgagtca cctggattta atctaactac cttttcaccc atacaaatat caaccacacc
     4561 actcgttacc accataattt cttctgatgc atgatagata taatctttag catcctcact
     4621 ttctggttta agttcaaact ctacaaataa aattgaaggt gtaaattcag ttgtaggcat
     4681 tggagatact atctcgtaaa acatttcact ctttggaaat ttcatcataa ctctatcttc
     4741 ttttctaatt actaaatcat cagtattatt gtcatccata aacatatata aaggagtgtc
     4801 taaaacttca gcgatttttc tcaatgaaga caaggatggt tcaatagcat ctctttctat
     4861 ttgagagata taacttatag ataaatctgt caatttggaa acatcactta atgttttttt
     4921 cttgcttttt cttatatttc tcatcttttc acctagcata agaacctcca aataataaaa
     4981 ttaatataaa atattcgctt aaaacaaagt aattatagca caacatagaa gtagtggcaa
     5041 taagaataca tagtagattt tgacaattaa aatttaatta aatccaaata aaagtctatg
     5101 aaaatgaatt
//
LOCUS       DAMJVX010000219         5053 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMJVX010000219 DAMJVX010000000
VERSION     DAMJVX010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.225"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            41..1813
                     /locus_tag="PC407_003345"
     CDS             41..1813
                     /locus_tag="PC407_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498754.1"
                     /translation="MENGKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSIS
                     LLVSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIE
                     TMMLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVV
                     LSQFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYK
                     EIMVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQ
                     GILKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSI
                     EHIKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTL
                     LKILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVY
                     KSNLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDA
                     DMYIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKL
                     LESGRHDELMDKKGVYFNLFCNCK"
     gene            1835..3256
                     /gene="ccpM"
                     /locus_tag="PC407_003346"
     CDS             1835..3256
                     /gene="ccpM"
                     /locus_tag="PC407_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HDF2498755.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVNKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            3285..4355
                     /locus_tag="PC407_003347"
     CDS             3285..4355
                     /locus_tag="PC407_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HDF2498756.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            4371..4622
                     /locus_tag="PC407_003348"
     CDS             4371..4622
                     /locus_tag="PC407_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HDF2498757.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            4673..4876
                     /locus_tag="PC407_003349"
     CDS             4673..4876
                     /locus_tag="PC407_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498758.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
BASE COUNT         2051 a          449 c          815 g         1738 t
ORIGIN      
        1 aaatatttta tttcaatttt attaagtatg gaggtgtatt gtggaaaatg gtaaatttca
       61 attttttaaa ggtgtaagaa attgttttaa tatgatttat ctacatgata aaagatattt
      121 atttatctct atgtttatgg caatttttca ggggattgtt ccatctattt ctttgttagt
      181 aagtcaaaat atcattaata atatgcaaac attttcgaaa agtgtaaaag aaatattagt
      241 tttgttaatt atgtatttaa tcataaatat aattccttca atttttcaaa gtttttatgg
      301 atattataga ataaagttta ataaaaattt tgaaaaacat attgaaacaa tgatgttaga
      361 gaaatcttta actttgagtt taaaagattt tgaggatgat aatacttatg acatgataaa
      421 tagagctcaa tctcaaaaag gatttaatat tactatacta tctgaaaacc ttatttcaat
      481 aataagagaa tcagtatcta cactttctct tattgttgta ttatcacaat ttcaactttg
      541 gctaataata gttgttattg ttattcctgt attgaaatca ttatattcca ttaaattagg
      601 aaaggaacaa tatgatgtta gtgtaaacag aacttggaaa gaaagaaagg cctggtacat
      661 agattatttg ttgtcaaaag gttatgctta caaagagatt atggtattta gaattggttc
      721 taaacttatt aatcattata aaaaattaaa agaggatatt atatctcaag atatgaatat
      781 agctaaaaag aattttattg ctacgctatt atgtacagtt ttagattcaa ttgtatctgc
      841 aggaattata ggatttataa taattcaagg tattttaaag aagattttaa taggagatgc
      901 gactacatat atacaagcag ttcaatctat aaaggataat gttgaatcaa tattttcgtt
      961 atttagttca gtaatacaag atctttttta tacagattta ctgtttaaat ttctagatta
     1021 taactgtaaa gaagactcaa ttgaacatat aaaaatattt gaaaaaatag gggatataaa
     1081 taaaatagag ttaataaata tttcatataa atacaatcat tcatcagagt attctttaaa
     1141 aaatattaat ttaactttaa ataaaaatga tcctattgct atactaggaa aaaatggttc
     1201 aggtaaaacg acacttttaa aaatattatt aggtttttat gatgattatg aaggtgacat
     1261 cttgataaat ggaaaaaatt taaaggatat tgaaaaagat gaatacatta gaaaagtaag
     1321 ttgtatgttt caagattata ttaaatatga ggctacatta aaagaaaatg tcgccatggg
     1381 agatgtatat aagagtaact tggaagaaaa agttaaaagt aaacttaaag aaactaaatt
     1441 gaaaccaagt atttatgagg aagatggatt agaaactatt ttaggaacct ggtttggtaa
     1501 gaagcaactt tctatgggtg aatggcaaaa agtagcaata tctagaactc ttatgaaaga
     1561 tgctgatatg tacatttttg atgaaccaga ttcatcttta gattctgaag cgggaaatga
     1621 actcctttct ttatataaaa aaacatttga aaataagatt ggaatattta taacgcacca
     1681 tatccaacat gcaaaaaaga taactaacaa tattgtagtt cttgaaaatg gaaagttgtt
     1741 agaaagtgga agacatgatg aacttatgga caaaaagggt gtttatttta atttattttg
     1801 taactgcaaa taaatattaa agggaggtta attaatgaat caaaaaccat ttatacacac
     1861 atttaaagct ggaaaatcat attttattta tgatgttaat acggataaga ttttaaaggt
     1921 aaatgctgct gtttataatt atctaaataa aattgagcat aatttagaaa aagaaagtga
     1981 ctggaacata gaaatagaag acgaaattaa taccttgatt aattatggat ttctaaaaaa
     2041 taaaagggta aataaaacaa tgcaccctga aactaagtat ataaaatatc atatagaaaa
     2101 taatctatcc tctattattt tacaagtaac ccaaaattgt aatttacgtt gtgactattg
     2161 catttattct ggttcataca gcaacagagt tcatacaaat aaaagaatga atatagatgt
     2221 agcaaaaaga agtattgact atttgattaa gaattctagt caattaaatg aagttagtgt
     2281 tggcttttat ggaggagaac ccttacttga gtttgatttg attaagcaga tagtagaata
     2341 tgtaaaagta gttggagaag gaaaggaaat atactttaat ctaactacaa atgcaacttt
     2401 atttaatgag gaaataatag aattctttgt aaaaaataat ataacaacta tgattagttt
     2461 agatggagga aaagaaattc atgataaaag tagaaaattt gcaaatacag gagaaggatc
     2521 tcatgatgtt gtattaaaaa atgtacgtta cataaaaaat aaatatccag aatattataa
     2581 aaagaatatt acatttaata cagtgataaa ttcagaaaat gattacattt ctatagatga
     2641 ttttatatct aaaaatgaga ctttaaaaga ttcaaacttt ttagcttcca ctcttaataa
     2701 taactattct gataaagaaa tagtttcatc agatgaatat atattaggta agagttatga
     2761 tagattcttg actttactca acaaattaaa atttataaaa gatgagagta tgtcaccttt
     2821 atcaagaata gaaagagcct tattagatga ttttagatat gagaaaaata ttgaaggtag
     2881 attagagtta ccagatgtgt ggcatcatgg tggaccatgt ataccagggg cttttagaat
     2941 atttgtaact acagatggag aattttttcc atgtgagaga gtaagtgaaa gttctaaaat
     3001 gggtatgatt ggaaatttag atagtggagt agatatagaa aaggctgaaa aacttttaaa
     3061 tataggaaaa ttatatgaag agcaatgttc tgattgttgg gcctatagtt attgtgatat
     3121 atgtatagct aagattgatg atactaaatg taatgaaaat gaaataaaac cacatgattg
     3181 tgacagaatt agacattcta cagaaaataa tatgaaagat tactgcgttt tgaaatctct
     3241 aggatatcaa tattaaattt tctaatgaaa ggaaagatgg gattatggaa aaagtactta
     3301 tatatccatt taattataat ttttttacta ttttaaatca ttgcaataaa atttcagata
     3361 tagaaattaa ttcactggtt agtccttctg gctgggggtt atgcaataag gagtataatg
     3421 ttaataatat tattactaaa gttacaaatg attttcaaag tgaacttaaa aagaattctt
     3481 tggtatggtt tgttgattca aaagaaaatc tagacttaga aaaatttata attcctaaat
     3541 taaaagtagc tctaaaagaa ggtaaaaaga tattatatac acgtgatgat cttaataaaa
     3601 tatctaggta tatatctcat tatgaattca aaaagattaa tgaatggaat ttagaaaaat
     3661 atgaaataag agatatagag ggtatatcac agataaacac accaataata tttatttgtg
     3721 gtctttatgg agggttagat acttttgatg taaatataag gataggagaa gaactaagaa
     3781 aaagggggtt ttcaatatta caatttggtc ctaaaaaaga gagctattta tttaaaatga
     3841 ttcctatacc agagtttatg tttgatgatt cgataagtag taagacaaag attattaatt
     3901 ttaaccattt tataaaagat aaagaggtag aggcaaagcc agatttaata attatagaag
     3961 ttccaggaga attattccct ttctctagaa aaatggttgg taattttgga attagagctt
     4021 ttgaaatttc taatgcagta ataaatgatt gtggtatatt atgtattcca tcaagtatat
     4081 atgatagaga atattgtgaa gaacttaaat attttattaa aggaagacat ggtataaata
     4141 tagattattt tagtatagag aaaaaagtta tagatacata tgagacagag gtgaagcaaa
     4201 aattttcata tatgtcatta gatgaaaact tttttgttga agatatagaa gattatgaaa
     4261 aaacattttt tatacatgat aataaacaat tactagaatt agtggacaat atagtgaatc
     4321 aattaaaaga gtatgctaaa gtaatatcta tttagaattg gaggttaatt atgaattaca
     4381 ctgaaaagga attactacta gagaatatat tttttgatgt gactggaatt aattttaata
     4441 aaaataacaa tttaaaaaaa gaattatttt tttcaccgtc tttacatata cagccaagag
     4501 aattactttt agtattttgc gaggtaatag aaaactttaa tataaaaatt ccagaggaaa
     4561 aacttttgaa taggcaattt acgagttttg ataatgttct aaaaataatc aatatggtat
     4621 aggttaataa aggtataaag taagtgatct tatagaaaag gggaggatat gaatgagttt
     4681 tggaaaaaga atagaaatga ataatatagg tgttgagaca agaggtgttt gtactattat
     4741 gtgtccttat acttgtaaga tgatggcatc ttcatcttgt aactgcaaga ataatgatta
     4801 taatgcaaga ggaggatcat ataatacata ttataataaa ttgatacatg gtcaaagaac
     4861 tcctgatgga atgtaaatat ttcagtaata agtatactat taaattgatt tagcatattt
     4921 gattaaattt atctttctta ctttatttcc ttttattata aaaatatgca gttgattata
     4981 attaagagtg tgattttata ataactgcat aaatagtatc tgttatataa atcacctctt
     5041 caattattat agt
//
LOCUS       DAMJVX010000220         5047 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMJVX010000220 DAMJVX010000000
VERSION     DAMJVX010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.226"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            30..1913
                     /locus_tag="PC407_003350"
     CDS             30..1913
                     /locus_tag="PC407_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDF2498759.1"
                     /translation="MFNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2137..2817
                     /gene="pgmB"
                     /locus_tag="PC407_003351"
     CDS             2137..2817
                     /gene="pgmB"
                     /locus_tag="PC407_003351"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF2498760.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2814..>5047
                     /locus_tag="PC407_003352"
     CDS             2814..>5047
                     /locus_tag="PC407_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_097546124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF2498761.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNKVASIEKYVSITSTRDYKESELLR
                     KCEDILNIELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNCIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKI"
BASE COUNT         2087 a          455 c          953 g         1552 t
ORIGIN      
        1 aaattattat aaaggaggga aattttaaaa tgtttaataa aaataaagaa aatataactt
       61 taagtaatca aatcaaagga acaattcaaa atagtttaac aaaagaagct atattacata
      121 taccaatgag caattatgcc tatggttatg atagagaaac actacatata agacttagaa
      181 ctaaaaaaaa tgaagcgaaa agagtaattt tacgaatagg agaccaatat gtgtgggata
      241 aaggaggtgc aggaggagga aatttaaatg catctggagt tggttggtct ggtggaacaa
      301 atatagtaat gagtaaagaa gtagaaactg aattatttga ctattggata gctaagtgta
      361 agcctttaaa taaacgctca cgatatggtt ttataataga aggtcaagaa gaaaaacttt
      421 tatttacaga aaaaagaata atagagttgg gaaatgaaaa tgatgaaaag gaattatgtg
      481 aaataggtaa tttttttgga tatccatatt taaattatat agatatacct aaagtgccaa
      541 attgggtgaa agatactgtt tggtatcaga tatttccaga tagatttgca aatggtgacc
      601 cgtctataaa cccagaagga gtagataaat ggggagctat tccaaccaga gataatttta
      661 caggtggaga tttacaaggt gtgatagaac acttagatta tttgagtgat ttgggaataa
      721 acggaattta cttttgccct attacaattg gaaaaaccaa tcatagatat gatacggtag
      781 attatatgga ggtagaccct acattaggag ataaagagac tttaaaaaaa ctaatagaag
      841 aagcgcataa gagaaatata aaaataatgt tggatgctgt ttttaatcat ataggatatt
      901 attctaaaca gtggcaagat gtagttcaaa ataaggaaaa ttctcgatat aaaaactggt
      961 tttatataaa agatatgagt aaaattgata ctccaataga acagatagat gaaaaaaata
     1021 taccttatga aacctttggg tgtgaaaaat atatgcctaa acttaataca gaaaattcag
     1081 aagtaataaa atatttattg gatgtaggca agtactggat acaggaattt gatatagatg
     1141 cgtggagatt agatgtttca aatgaagtag accatgtttt ttggagaaag tttagagaag
     1201 aagtcaaaaa agtaaaacca gacatttata tattgggaga aatttggcat ggtagtttac
     1261 catggctaat gggtgaccaa tttgattcag ttatgaatta cttgatgtca gaagccatga
     1321 aaaaattctt ttgtacagat gagataaacg ccgaagaatt taagtatatg ataaatgatg
     1381 taatggtaag ttatccaata caagttagtg aagttatttt taatttactt ggaagtcacg
     1441 atactacaag aatattgact tatgctaatg gaaatattga tagatttaaa cttgcatata
     1501 tgtttatgtt tgtacaaaca ggatgtcctt gtatatacta tggtgatgaa atcggaatgg
     1561 aaggagaatt aagttcaata tctgaaggtc aaagaaaatg tatggaatgg aatgaagaaa
     1621 aatggaataa ggatatattt gattttatga agaagattat tagacttcgt aaagaaaata
     1681 aagaacttag gtctatatca aatgaatggg tgttagcaga taaggaaaat ggaacgatta
     1741 tattgaaaaa agaatctatt tctataataa taaataattc atctaaaaat ataattttga
     1801 cgttaccaga ttatttagca aataaaaaag taaaagattt atatgaagaa gaaatagttg
     1861 atttaaaaga agaactaatg ttagagaaat acaagttcat tattttaaaa taatacaaaa
     1921 agtatttatt ttagatttca tgttaaatta ttatacagtt taaacaagtc tttataaaac
     1981 ataaaaaaat atggtttaca agtgagtgaa ttgaaagtag attttaaatt aaagactgag
     2041 ttagagtaga taattttata aataatatct taaatacaag atagaaaata aattgattat
     2101 tagtctaaaa atttcttgta gaaagaggaa ataagaatga ttgaagcatt tatatttgat
     2161 ttagatggtg taattacaga tactgcatat tatcactata tggcttggag aaaactagcc
     2221 cataaagttg ggatagatat agacacaaaa tttaatgaat ctttaaaagg cataagtaga
     2281 atggagtcat tggacaggat attagaattt ggaaataaga aatattcatt ttcagaagaa
     2341 gaaaaagtga gaatggctga agaaaaaaat aattactatg tatcattgat agatgagata
     2401 acttcaaatg atattttgcc aggcatagaa agcttgttaa ttgatgtaaa atctaataat
     2461 ataaagatag gtctatcttc tgcaagtaag aatgcaataa atgtattaaa tcatttgggt
     2521 ataagtgaca aatttgattt tatagctgat gcaggaaaat gtaaaaacaa taaaccacat
     2581 ccagagatat ttcttatgtc agcgaaaggg cttaatgtaa atccacaaaa ttgtataggg
     2641 atagaggatg caagtgcagg tatagacgct ataaactcag ctaatatgtt ttctgttggt
     2701 gttggaaatt atgaaaactt aaaaaaagca aatttagttg tagattcaac aaatcaatta
     2761 aagtttgaat atatacaaga aaagtataat gagtatatag ttaggagaat tatatgaaat
     2821 gtttatttag taatgtagat gagtggaaga ttatacaaga taagattgaa atcaaagaaa
     2881 atagattagc agagtccata atgagtatag gcaatggata tatgggtatg agaggaaact
     2941 atgaggaaaa ttatagtgga gatacacaca aggggtctta tatagctgga gtatggtttc
     3001 cagataaaac tcgtgttggt tggtggaaaa atgggtatcc agaatacttt ggtaaaattc
     3061 ctaattcagt caactatata ggtgtaagaa ttttcataga tgatgtagaa ttagatttag
     3121 caaaatgtaa agtagaagat ttttatagag aattagatat gaaaaatggt attttaaatc
     3181 gtaggtttat tgtaaatata aatgataaaa aatttgaagt tagggttact aggtttttaa
     3241 gtatatctgt taaggaactt gcagtaatta aatatagtat aaaatcatta aattctaata
     3301 ctaaaataaa atttgtacca tatttagatt caaatataaa aaatgaagat tcaaactatg
     3361 aggaaaagtt ttggaatgga atttcttcaa gttctttaga atataaaggt agtattcttt
     3421 ctcgaacaaa ggagaatgca tttaatacac cagtattcac tgtgggttca atgatgtata
     3481 ttaagacaaa tggtgatgta gaagctgtat ctcatgagag tgattataat tactgtgaaa
     3541 attcgataac aatggatgtt ttaaaaaata aggttgcaag tatagaaaaa tatgtatcta
     3601 taacttcaac tagagattat aaggaaagtg agctattaag aaaatgtgaa gacattttaa
     3661 atatagaact atctaaaagt tatgatgata tattaaatga acatacaaag ttatggaata
     3721 aaagatggga aagtgcagat gtaaaaataa atggagataa aagtagccaa caaggtataa
     3781 gatataattt attccaacta ttttcaacat attatggaga agattcgcga ttaaatatag
     3841 gtccaaaagg atttactggt gaaaagtatg gaggagctac ttattgggat acagaagcat
     3901 tttgtgttcc agtatatcta ggtgtagcag ataaggaggt aactagaaat cttttagaat
     3961 ataggtacaa tcagttagaa caggcaaagg ataatgcaaa aaaactaggg cttaaaggtg
     4021 ctttatatcc aatggtaaca tttgatggag tagaatgtca taatgaatgg gagataacat
     4081 ttgaggaaat ccaccgaaat ggaagtatag tatatgcaat atataactat acaaattata
     4141 caggtgacta taaatatata aaagaaaaag gaatagatgt aataatagaa atagctagat
     4201 tttgggcaag tagagttcat ttgtctacaa aaaaagattt gtatatgata catggagtaa
     4261 caggtccaaa cgagtatgaa aacaatatca ataataattg gtatactaat tgtatagcta
     4321 aatggtgttt agattatgct gtagaaaata tcttacgatt agagaaagag tgtaatgaat
     4381 gtattgacag aaataatgtg actagggaag aagttgagtt ttggaaaata atatcaaata
     4441 aaatgtatct gccgtatgat gaggaactac aaataataat tcaaaatgat aattttttag
     4501 ataaagaatt tgtagaagta aaggatttac caaaagaaaa ttttcctctt aatcaaaatt
     4561 ggtcttggga taagattctt aggtcgtgtt ttataaaaca agcagatgta ttacagggaa
     4621 tttactattt tggaaataga tttactaaag aagaaaaaaa gagaaatttt gacttctatg
     4681 aaaaatatac tgtccatgaa tcttctttat cttctagtat ttattctatt attgcctcag
     4741 aaatagataa cctagaaaag gcttatgaat tatactcaag aacagctagg cttgatttag
     4801 ataattacaa taatgataca aatgatggac tgcacataac ttcaatgtct ggttcatggc
     4861 tttccattgt acatggattt gcaggcatga gaacatggaa tgaaactcta tcttttgacc
     4921 ctaagttgcc aaaagagtgg aaaaattatt ctttcaatat aaattataga gataatatta
     4981 ttaatgtaga tgtatgtagt gatttaatta aaatagaaaa tttaaaaggt aaaccaataa
     5041 aaattat
//
LOCUS       DAMJVX010000221         4997 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMJVX010000221 DAMJVX010000000
VERSION     DAMJVX010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.227"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            114..1517
                     /locus_tag="PC407_003353"
     CDS             114..1517
                     /locus_tag="PC407_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2498762.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIIFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLTLANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGSK
                     FVGGKSIVFSIIGIIFLIILISGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNKYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            1737..4214
                     /locus_tag="PC407_003354"
     CDS             1737..4214
                     /locus_tag="PC407_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2498763.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDVLTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEMTENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIENVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDVVMIQIRDLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKKRSIAMYKSIGLSDK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            4229..4915
                     /locus_tag="PC407_003355"
     CDS             4229..4915
                     /locus_tag="PC407_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498764.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALVGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
BASE COUNT         1929 a          532 c          796 g         1740 t
ORIGIN      
        1 atttatgtag atagttgtat ttttaaataa atttttacat atcaataatt attatctatt
       61 ttatataagc aacaatcaaa ctaaaataaa agtttaagaa aagagttgat taaatgcata
      121 aacctagtta taaaacagta tttcattcat acattatttt tttcatgatt tttatttgct
      181 taatcctgac agtaataggt attttcttat cacttattac tattcagaaa ccaaatgggg
      241 aatttattcg tagtgactgg ccgaaaaaat ttacagaaaa ctttaatgaa caaatcatat
      301 ttatagataa taagccacaa gtaaaacagt ctggattaga aaagcttcga gaaaatcaat
      361 tgtggttgca aattttagat tctagtggaa atagagttta tgggttttta gaaccaaaag
      421 agcataaaga ttattattct aattctgaat tattaacatt agctaaccaa aataattcaa
      481 gtaatggaac tgtatacttt aatacaatta aaaataataa aaatgactat atttatatta
      541 tatattttcc attaaatata tctaaagtga atatgttttt tgatggaagt aaatttgtag
      601 gtggtaagtc aattgtattt tcaatcatag ggattatatt tttaataatt ttgatttctg
      661 gaattgctta tggttactgg gtaacaaagt gcatatcaaa tattacatct gctgttagtg
      721 atatttcaaa acgaagctat cttccagtta atattaaagg tgtttttgct gatatatata
      781 atagtttaaa tattttaaat gaagaaattc atagaagtga tgagatacaa aataaaactg
      841 atattatgag agaagagtgg atttcaaata taactcatga tttaaaaact ccactttcac
      901 caataaaagg gtatgcagag cttcttacag atagtagtac taattattca cctgaaaaag
      961 tatataaata tgcttcaatc atgttaaaga atatagaata tacaaataca cttataaacg
     1021 atttaaaatt aacctatcaa ttaaaaaatg gaataattcc tttaaataaa aataaatatg
     1081 atttagtaag attcttgaaa gaattatcca ttgatatttt aaattatcca gaatatgaat
     1141 caaatatagt tgaatttaag agtaatatag ataatattaa ctttgatttt gattgtacac
     1201 tattaaaacg agcttttagt aatcttattg tcaatgcttt tacatatgga gataaaaata
     1261 ctaaagtttg tgtacatata acattaaatg aaagaattga aatcattata tctgataatg
     1321 gaaaagggat gtcaagagaa gaagcatcaa atttgtttaa tcgttattat agaggaagca
     1381 atacaaaaca aaaaatagaa ggaactggtt taggactagc aataacgaag caaattattg
     1441 aagttcatgg agggaatata tctgttgaaa gtatacctgg attaggtagc tcattttata
     1501 tcacctttcc tatttaatta aggttaatta aggtcaaata aaaaatatat taaggttgat
     1561 agattattat atttatatag tagtttatca atctttttta tttaaggagg tgatttgatt
     1621 aaaaatatta taaatagaat gataaaagta tggaggagtc tattagagat tcaatttgga
     1681 ttaaagtata aaaatattat actgaaatat cttagataaa ggaagaaggt gtaaatttga
     1741 aaattatatt aaagtatatt ttcacaaata ttaaagaacg aaaaattaga acagcagtta
     1801 tgttactgtc catagtttta tcaacagttt tactttttgt ttcattctca attggattat
     1861 cttatgaaag tgcacaaaga aaaatggcaa aaggtatgta tggtacagcg actatatctg
     1921 tccaaagtaa aaatccagat gtattaacca atttagaaga catacctgat ttaaatgcta
     1981 taaaatcaaa ggttggtgta ttggaaagct ctgctattta taataaaggg ggatactatg
     2041 aagaatttag tcttatatca gcagacttaa gtcaactaaa taaaatcaac aagccacgat
     2101 tagaaaatgg cgatagtatt actgattttt ctggtgataa aattattctt ccaaatcgct
     2161 ttacatcaaa atataaaata aaaaaaggtg attctattac tttacagatt tatggaaagt
     2221 cttatacttt tcaagttagt gatattgcat cttatgatac agtatttttg aggaatacaa
     2281 gaggtgtaaa tgcactgcta cccaaagaga ctctatcaaa aattataaat aaaggcagtg
     2341 gatatacaag agttcttata gagtcagaag aagaaatgac agaaaatcta gtaaataaat
     2401 tatccgaaga actttccact gaaaaatata tggtatcaaa tactataaat gaaactaaga
     2461 taatttcaga tgctagacaa aagacaatgc ctttcttttt aataagtttt tttgcattaa
     2521 cccttagtat atttatcata tacagcagct ataaggttat tacattagaa cgtcttcctt
     2581 ttattggaac atttagaagt attggtgcca atgaaaaaac tgtaactcgt atattgatgc
     2641 ttgaaagtat attatacgga agtataggag gattaattgc tattcctatt ggtgtagttg
     2701 ttttaaattt aatgcttcat gggttaggaa attctctcga acaaggaata tcaattccag
     2761 tagtaatatc acccattgga gtgattatat ctgttatagt agcaataatc gtttcttcat
     2821 ttagtgcata tatacctgtt aaaaaagcta gccatttgcc aataaaaaat attgtactag
     2881 gaacagtaga agaaaagaat gtttcaaatc gttctatact ttttatagga agtataatgt
     2941 ttattttatc tattttactt ccaagaatat cccctgaaaa tacattgtat ctagctggtg
     3001 gattttcatt actaggatta attgtagcta ctattgtgtt gattcctctt attactgata
     3061 tcatgtctat agtttttgag tttgtttata aaaatatttt agggaatgag ggtaaattag
     3121 ctgctagaaa tatgaaaaat aacaaaaata taatacagaa tatcacttta ctatttataa
     3181 gtatttctgc agttattgca ataagtgttg ttggtaactt tgtcaaaact tatataacag
     3241 atgtgtttcg tgacgcagaa ttacaaggtt ttgcagatgg aaagatgaat gaagagttta
     3301 ttgaaaatgt tagacatatg gatggaatta aaaaaatatt acctctttat gttatgaata
     3361 atgagatttc tggtaatggt gtaacattat caagactaga gggtacagat aatataaaac
     3421 tatataactc tatgtttgga atcaattata caaattttga gataaaaaaa caagtaatag
     3481 aagcttttaa tgataaacgt tctgttattt tgaatgaaga tactctaaaa aaagtaggct
     3541 tatctatagg agatacaata actctatcta atgataagta tgatttttct tataaaatag
     3601 taggtagttt taaatcccgt gcaaatgatg tagaagctgt tataccatca cattatgcag
     3661 ttaatgattt tgataaaact aattatggtt ttttagtata tacagcagtc aatccagatg
     3721 ttgttatgat tcagataaga gatttatttg gagatacata taattggagt cgtacagttg
     3781 aagaatttaa taatgattct ttaaatacta taagtagttt tttgtctcct atgaataaga
     3841 tgacctattt catttttctt cttgctacag taggtattat taataatcta ctgattaatt
     3901 atattcaaaa aaaacgttct atagctatgt ataaatctat aggactaagt gataaacaaa
     3961 atataaaagt gacattgatt gaagggttta cttcaggttt acttggagct gttattggta
     4021 ttgtgatatc tatacttgag attcaaacta tctttatagt tgcaggacca aaaatttcaa
     4081 tgaaaccaga tttagatttt aaaacattta ttattgttgg gttattggga attatagtaa
     4141 ctttaatagg ttcgatagtg cctattataa aagggaagaa aatgaaactg atagaagaaa
     4201 ttaagtttga ataattatgg aggttaatat gaaaattaca cataatagtt tagctgtaga
     4261 agcaaaaaat attatcaaag aatataaaat tggaaataca acaactcgag tgttaaaaga
     4321 ggtatcttta caagtaatga aaggtgaatt tgtttctatc atgggtcaat ctggttctgg
     4381 taaaagtact cttttatata ttttaggtgg tttagatact ccaacgagtg gaaaagtgta
     4441 tatgaatgga gctgatatat cacattttaa tgatgagaaa atgagcataa tacgtcgtag
     4501 aaatatagga ttcgtatttc aattttataa cttaatacca aatttaaatg tggaggaaaa
     4561 tataatgctt cctttgttat tagatggtaa aaatttaaaa gattataaaa atcaattaga
     4621 tgaaatctta gatatagttg gtttaactga tagacgtaaa catacaccac gagagttatc
     4681 tggtggtcag caacaacgtg ttgctatagc tcgagcttta gttggaaaac cagagatttt
     4741 atttgctgat gagcctactg gaaatttaga tagtaaaaca gggatagaaa taatagattt
     4801 actgaataaa ataaacagag acaatggaca aactataatt atggtaactc actcaccaga
     4861 agcagccaaa agtagtagtc gtactattac agtttcagat ggtttaattg tataaggact
     4921 aattattgta tttgagataa gcatttttaa ctatataaat tagacatatg taaagtggat
     4981 ataagttaaa tactata
//
LOCUS       DAMJVX010000222         4997 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMJVX010000222 DAMJVX010000000
VERSION     DAMJVX010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.228"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(463..2598)
                     /gene="rnr"
                     /locus_tag="PC407_003356"
     CDS             complement(463..2598)
                     /gene="rnr"
                     /locus_tag="PC407_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDF2498765.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILAKILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
     gene            3194..3808
                     /locus_tag="PC407_003357"
     CDS             3194..3808
                     /locus_tag="PC407_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2498766.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            3826..4188
                     /locus_tag="PC407_003358"
     CDS             3826..4188
                     /locus_tag="PC407_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2498767.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYKGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            4294..4791
                     /locus_tag="PC407_003359"
     CDS             4294..4791
                     /locus_tag="PC407_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDF2498768.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT         1630 a          756 c          603 g         2008 t
ORIGIN      
        1 ctattaaatg tttctcaatt acttattttt caattagcca cttctattaa acatttctta
       61 attacttatt tttcaattag ccacttctat taaacatttc ttaattactt atttttcaat
      121 tagccacttc tattaagcat ttcttaatta cttatttttc aattagtcac ttccattaaa
      181 catttcttaa ttatttattt ttcagttggc tttttctatt aaatgtttct taattactta
      241 tctttcagtt agctctttct attaaatatt tctcaattac ttatttttca attagctact
      301 ttcattaaat atttcttaat tatttatctt taagttaact atttccatta gatatttctt
      361 aattacctat ttttatcatt tattttatag agtattgtgc actatatatt tatacatatc
      421 ataaatacta caacttgtta tctcttattt tagctgtttt atctactcct ctattttttc
      481 aactatctca aagtcaattt ctttaaaatc tacattaaca ctttcaacct ttatcttaac
      541 aaggtctcct atcttatatg acttcttagt tctttctcct aaaacactat aagaactctc
      601 atcataaata tagtaatcat cattcatatt tgcaagtctt atcaatcctt ctatagtatt
      661 atctaactca acaaatattc caaatgaagt tacgctagat acaacaccct caaattcttg
      721 accaacttta tccttcatat aaagagcctt atagaaatca tgtacatctc tttcagctaa
      781 ctctgccttt ctttctttct cggatgattg aacagatgca taatccacta tagtagtaag
      841 ctgttcttgt ctttttccac ttattttatt atttaaagat tcttttatta ttctatgaat
      901 ttgaaggtca ggatatcttc ttattggtga agtaaagtga cagtagaatt tagctgctaa
      961 tccaaaatgt ccactacatt ctggtgagta cttagcttgt tttaaagaac gaagcattat
     1021 agtactgata gctccttctt cttttttgcc ctttattttc tctattatac cttgtaatgc
     1081 ttttggatgt acttcctctt tatcaccttt tattatatat ccaaatgtag atataaactt
     1141 actcaattct tccatttttt ccaaagatgg tgtctcatgt attctataca caaacggtat
     1201 tcccatccag taaaaatgtt cggcaattgt ctcattactt ataagcataa attcttctat
     1261 tatcttatta gatattcttc tttcatatgg ctttatatct acaacttcgc cttttccatt
     1321 tagaattatc tttgcctctg ggaaattgaa gtctatagct cctctttttt ctcttcttga
     1381 cataagaatc ttagctaaaa tttcagcatt tttaaaatct tcaacaacat gttcaaaagt
     1441 ctttttaagt ttttcatcat ccttttctaa tatatcagat acttctgtat aagtcattct
     1501 agcttttgaa tttattacag attcacatat ttcatgttta accacatttc ctttatggtc
     1561 tatttccata aatatagaaa gagttaactt atcttcaaat ggatttaagc tacatactcc
     1621 atttgaaaga gtttttggca acattggaat tactttatct actaaataaa ctgatgtagc
     1681 tctttttaag gcttccttat caagtttatt tttctctctt acataatgtg taacatcagc
     1741 tatgtgaact cctagtttga agtttccatt aggtaatact tctattgata tggcatcatc
     1801 taagtctttt gcgtcatctc catctatagt aaatatgttt aaatctctta aatctcttct
     1861 tctttttatc tcttcttgag gaatctcaac agctacagct tctgcttctt gcaatacttt
     1921 ctttggaaat tcttctggaa gaccatgttc tcttattata gaatctattt ctacgcctct
     1981 ttcacctttt tgacctagta tctctataat tttgccttct ggttttctat cctcttgtgg
     2041 ccatactgta atttcacata ctaccttatc attatctact gctccactaa aatgtttttt
     2101 aggtatatat atatctttag taaatttctt gttgtctggt gttacaaatc caaaagtttt
     2161 acttgattgg aaagtaccta ctattctagt tatttctctc tttattactt ttactattct
     2221 tccttctgct ctcttttctt cagttgcagg tactactatt tctgccataa ctctatcgtt
     2281 atgcatagca ccatttatat tgtcacttga gataaataaa tcctgtgtat attcaacatc
     2341 agattctaca aatccaaaac cctttttgtg tgatacaaat tttccaacaa agtatcccat
     2401 ttgattggga gatactattt ttcctttttt agtaaagcag atataaccat cttcttctaa
     2461 ttcatttaaa aaattataaa acattggcat ttctgatgaa tgtatatcaa atatttctgc
     2521 taattcttct ttttttagag gattatatgc actttcattt attaatccta gcaaactttc
     2581 tttaagtcct ggtatcattt ttccctcctg ttttactttt cattatatta ttatttatat
     2641 tgtcttttat atttcttcag ataaaacatt ttttacttat cacataattt tctatatttt
     2701 cttattatta ttacaagtta aaattactta tttatatttt tgatttaatg taagctttgt
     2761 catactaact tcaaacaaat tatgatgttt tataaattat ttgtaaatat cattataatt
     2821 acttaagtta ttctttatca taattgctta aattactctt tatctcataa ttatacacca
     2881 tcaaatagga ttggtaaaat attcatctca tataagtatt actatataat aatttattct
     2941 attagtatac tcaaatcatg taaaatttat ataactcaat taatttttta aatacaactt
     3001 tataatataa aacttataat aaaagtaaat ttatatgtta aatgaggttg attttttatt
     3061 attttttatt tatgctagta ctaaagactt gcaaacgttt tttgacaagt ctttattttg
     3121 tctaaattaa acaaactttt ttacaaataa atacataaaa tttaaactaa tctatttatt
     3181 ggaggagata aaattggaag aagtaattgc aaaaattatt actatagaaa atggattcaa
     3241 agaaattgaa aacattgcta aaaaaattgt aaaaaataat gactcaaaaa agtgttatta
     3301 tctatcaatc tacttatata ggtcagaata ttatcaagta agagaattgg ctgtgtttat
     3361 acttggtttt atatcagtaa ctatttctga agctttgtta tttttaaaag acaatgtgag
     3421 taaagatgaa aactggagag tacaagaaat actagctaaa tcatttgatt attattgcag
     3481 tgaagtagga tatgaaaagg cacttcctgt tataaaagaa tggcttaatt ctgatagtcc
     3541 aaacataaaa agggctgtga cagaaggtct tagaatttgg actagtagag actattttaa
     3601 atctaatcct gacgttgcta tatcactact ttctagttta aaagaagatg atagcgaata
     3661 cttgagaaag tctgtaggta atgctcttag agatataagt aaaaaatatc cagatttagt
     3721 aaaaaaagaa ttagattcat gggatatatc aaataaaaaa gttgagtttg tatataagct
     3781 tgcttcaaaa tgtatcttgg caaagtaaaa tggaggtgaa cttatttgaa acttcgtgat
     3841 gagtatactt gcccattaga actaacacat gatatgataa aaggcaaatg gaaatctatt
     3901 atattatgga gattacgcat aggaccaacc tccttatcaa aacttgaaaa ggaaatcaat
     3961 ggtattaatc aaaagatgtt acttgaacaa cttaaagaat taattgaatt tggtcttgta
     4021 gataaaaaga catataaagg ttacccactt aaagttgaat actcacttac acctattcga
     4081 gggaaacaaa tattggatgc tttggctata atgcaagata taggaattag ctttatgttg
     4141 gataatggta tggaagatat tcttaaggaa aaaggtgttt taaaataaat agatacccac
     4201 aaaaaagtaa gtaatttacc attattttca actggtatat aattaaatta tagatatatt
     4261 ctattaattt attataatag gaggtaaatt attatgaaag taactagtac aggtatagta
     4321 aatggtgtta tagaagataa atacggtaaa agaggaactc agttcaatga aggtggtatg
     4381 cctacatatt cactacctct aaagtttgaa gatgctccaa agaatacagt atcctttgct
     4441 gtatttttag aagataaaga ctctgttcct gtatgtgggt ttgcatggat acattggtta
     4501 gctgtaaata taacaaaaga tgagttaaat gaaaatgaaa gtgtaacagc tactgatttt
     4561 gtacaaggga ctactagttg gcatggcaaa ctaggaggtc ttgacagatt agaggccagt
     4621 gtatatggtg gaatggctcc tccagacaga cctcatgtct atgaaataca tgtatttgca
     4681 cttgatacta tccttgacct tgaaaaagga ttctatatga atgaattttt caaagctatt
     4741 gaagggcatg ttttagatac atttacacta aaaggaagct attctaatta attaggttta
     4801 ctatagctaa tcattaatta aatgctacat ttaattgatg attagcttaa ttgttattag
     4861 aatattttct gcactttatc ttaatatata ttttagttga ctggtatttt aatttctatt
     4921 agattattat caatttatat aaattgattt tagcttaaaa cttacttatc tatttcagtt
     4981 tctattagaa ttattat
//
LOCUS       DAMJVX010000223         4932 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMJVX010000223 DAMJVX010000000
VERSION     DAMJVX010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.229"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     misc_binding    234..465
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            573..2054
                     /locus_tag="PC407_003360"
     CDS             573..2054
                     /locus_tag="PC407_003360"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDF2498769.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKKFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            2064..2957
                     /locus_tag="PC407_003361"
     CDS             2064..2957
                     /locus_tag="PC407_003361"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDF2498770.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIHSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQNYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            3081..4142
                     /locus_tag="PC407_003362"
     CDS             3081..4142
                     /locus_tag="PC407_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDF2498771.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(4297..4809)
                     /locus_tag="PC407_003363"
     CDS             complement(4297..4809)
                     /locus_tag="PC407_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDF2498772.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKIYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
BASE COUNT         1836 a          609 c          767 g         1720 t
ORIGIN      
        1 tgtaatatta cagatattta atagttatct tttgattact ttcattaaaa acagcttcat
       61 cactatgaaa atctaggttt ttaaactttt aaaaaagtta aaaaaatatt tttctaatac
      121 tcaatttgat tacaattttt ttacaattta aaaaatattt tttccaaatt tttatattaa
      181 tacttgactt tgtctgtttt ataaactaga ataattaata atattaagaa tattgaaagg
      241 cattgattag gaagagtaaa tctgaatcga ttaccagaga aggaaactac tagctgaaat
      301 gtttccctaa ttagaaggat tgaaaactac ctaggagctt ctatccgaag ttatcttaat
      361 gattttagta ggaatagacg tttttattac ttgcgttaaa gtatcaagtg gattttttaa
      421 aatcaatttg ggtggaaccg cgggaaacag attctcgtcc cttttttagg gatgagggtc
      481 tttttttatt acctaaattc ggtttttaaa atattctgta tctaatttaa ttagctaatt
      541 aaaaatttaa aatatatgga ggattataaa aaatgtatta ttcaagtacg agaggcaccg
      601 aagaaaaagt aactgcatca caagcaataa taaaaggaat atcaagtgat ggtggtcttt
      661 atgttccaag tgaatttccc aatgtaaaaa atgaacttat aaatttagta aatttaacgt
      721 attctcagat agcatttttt gtacttagta aatttttatg tgattttact gaagatgaaa
      781 ttaaaaattg tatagaaaat gcatatgatg aaaagtttga ttgtagtagt atagctccat
      841 taaataaagt aaatgatact tatttcttag aactatatca cggtccaact ttagcattta
      901 aagatatggc acttactata atgcctcatc ttcttaaaac atctataaag aaagataatt
      961 tagagaaaga tgttgtgatt cttactgcta catctggaga tacaggcaaa gctgcacttg
     1021 agggatttaa agatatagat aaaataaaga taattgtatt cttcccagaa gatggtgtaa
     1081 gcccagtaca aaaacttcaa atgaaaactc aaactggaaa aaatacatat gtagttggta
     1141 taaaaggaaa ttttgatgat gcacaatcag gtgtaaagaa gattttttct gataaaaaat
     1201 ttaatcaaaa actattggat aataattata tattatcttc tgcaaattca attaatatag
     1261 gaagacttct tccacaagtt gcatactatt tttattcata tatgaaatta gttagcaata
     1321 gtgagataaa tttaaatgat aaaataaatt ttgttgtccc aactggtaat tttggtaata
     1381 ttctagctgg atattatgcc aaacaaatgg gtcttccaat aaacaaattg atatgtgcat
     1441 caaatgataa taatgttctt tatgatttct ttaaaactgg tgtatatgat aaaaatagaa
     1501 cattgaaatt aactacttct ccttctatgg atatattaat ttcaagtaat ttagaacgat
     1561 tactgtttga aatatcaaat agaaatactg atattgtaaa taagctgtta tcagacctta
     1621 gtgataaagg agtctacaaa ataagtgatg atatggaaaa aaatttagcc tcattctatg
     1681 gagaatattc aaatgaagaa gaagttaaaa gcacgataaa caatgtgttt aaaaattata
     1741 attatttgat agatactcat actgctgttg catacaattg ttatgaaaaa tataaaaaag
     1801 aaacttctga taacacaaaa actgttattg taagtactgc aagtccattc aagttttctg
     1861 aagatgttct aaagtctata gattgtgatt ttaaagacct tgatgacttt gctataattg
     1921 atagattatc aagtatttct aaaatagata ttccaaaacc tattaaaaat cttaaaaatg
     1981 cagaaatact acataaagat atttatgaaa aagatgaatt aaaactagct ataaaaaaat
     2041 tcttaaaggt ataggtgatt attatgttag agattatagt tcctgcaaca agtgctaata
     2101 taggtccagg atttgattgt cttggaattg cgttaaatat atacaataaa ttttatgttg
     2161 aagaaattga aagtggtctt gaaatagaag gttgtgaaga tgcttacaaa aacgaaaata
     2221 acttagttta tacatctatg aagtactttt ttgatagagt taaaccagaa aaaatacctg
     2281 ctggtatcaa aataaaaatc cacagtgaag tacctatatg tagaggtctt ggcagtagtg
     2341 cctcttgtat agtagctgga gttatagctg caaatgctct atcaggtgct aatttagata
     2401 aaaatcaatt attaaatata gcttcagaaa tagaaggtca cccagataat gttgctcctg
     2461 ctattttagg aaatatgatc gtatctgtta ctgataatga gaatatacat tatgatatta
     2521 taaaaattcc tgaagaactt aaattttgtg caatgatacc taattttaag ttatcaacag
     2581 aaaaagctag aggtgtttta ccaaaggaaa taccatattc tgatggggta ttcaatgtta
     2641 gtagagttgc tttactaata tctgccttac taaacaaaaa ctttgaccta cttaaagtag
     2701 cttgtcaaga caaattgcat caaaattata gaggaactct aattgagaac tacaatgata
     2761 ttgttgaaaa atctgaacaa ttaaatagta ttggagtatt tttaagtgga gctggaccta
     2821 ctataatgtc tttaataaaa gaaaatgatg atagttttgt ggataatatg aaaaattacc
     2881 tacaaaaact aaaatctgat tgggaaataa aagagttatg ttgcgattct aatggagctg
     2941 ttttaaatat aatataatta tcgtcttcta tacaaaatat aactactctt ttattataat
     3001 ttgtagtata ataattagga ctaggtaata atattaagta ttataatcta gttctttaaa
     3061 catgtttaca ttgaggaggg attatcataa aagagttaat aattagagat ttagttgcaa
     3121 aagttcctat aattcaaggt ggaatgggcg ttggtatatc taggtcatca ctagctggtg
     3181 cagttgctaa gcttggtggt gttggtgtaa tttctggagt tcaaattgga tatgatgagg
     3241 aagattttga aactaatact ataaatgcaa atttaagagc tataaaaaaa catatttcta
     3301 aggccaaaga aatctcaaat ggtggtatta ttggtattaa ttttatggta gctatgaaag
     3361 aatatgaaac atacgtaaag gaagctgtta aagctggtgt agaccttata atatctggag
     3421 ctggattacc taataagcta ccatcattag taaagggaag taatgtaaaa atagcaccca
     3481 tagtttcaac tgcaaaagct gctaatgtaa ttttaaaaat gtgggataga aaagagaaaa
     3541 ctactgctga cttaatagtt gttgaaggtc caaaagctgg tggtcacctt ggatactcta
     3601 acgaggaact tgataacata gattctatag actatgataa ggaatttgtt gaaattttaa
     3661 aggttgcaaa tacttatgga gaaaaatttg gtagaaacat accagtagtt gctgctggtg
     3721 gtataacttc tagtagtgat gttaaaaaat atatagatat gggtgcaagt ggtgtacaag
     3781 ttggtacaag atttgttgct acttacgaat gtgatgccca tgagaacttt aaaatggcat
     3841 acataaatgc tagtgaagaa gatgttcata tagttaaaag tcctgtaggt cttcctggac
     3901 gcgccataag aaacaaattt atagaagaag ttaaaataaa tcgtccagaa ataaagaagt
     3961 gctataattg tttgattcct tgtaatccaa aagaaactcc ttactgtatt tctcaagctc
     4021 taatcaatgc agttaaagga gatgttgaaa attcactttt attttgtgga aatgatgcct
     4081 ataaaattga taaattaagt actgttgaag atgttatcaa tgaattgatt tctgaattat
     4141 aatttaaaga aaactaaatt aaaattttat caagctaagg agttttaata cgttatttat
     4201 taatctttat aaacatattt atgtccttag cttttttgtg tcaatttcct tttattgaaa
     4261 ttttaattta attagagata gtattaaccc ttataactaa tcttctttta caagtacatt
     4321 ggttatttca gctatatata aagtatgata atctttttgt ggataccatc ttccatctat
     4381 accttcagca ataaaacatt ggggttcaag tttctgacta taaatttttt tgcatataat
     4441 agccatttta gcttccttaa aataaggtgt atcatttaag tgttctatat taaatccaat
     4501 ctgtgatatc ttatcctctt ctctaccaga tacctttcca caatatgaaa gctctttacg
     4561 aaaatcttca tcaaaaaatg taagagaaaa tttatctgta ttatctataa attccttagt
     4621 ataacgctgt ggtcttatta ctacaaaagc aacatctttt ccccacataa ctccaagtcc
     4681 tccccagcta gcagtcattg tattcacttt accttcattt tctgctgtta taagtgtcca
     4741 atctttacct atcaatccaa atggattatt tttaatctct tctgccaaaa tttctttaaa
     4801 atcactcata tctctaataa cctctcattc tcttatattt atattccttt tattttcgtg
     4861 ataaattata aatgaagtct agttttgaaa ataaaatact tttttaaata tattattgca
     4921 aataagtaaa ta
//
LOCUS       DAMJVX010000224         4931 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMJVX010000224 DAMJVX010000000
VERSION     DAMJVX010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.230"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            160..1311
                     /locus_tag="PC407_003364"
     CDS             160..1311
                     /locus_tag="PC407_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDF2498773.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDVVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1357..2532
                     /gene="thiI"
                     /locus_tag="PC407_003365"
     CDS             1357..2532
                     /gene="thiI"
                     /locus_tag="PC407_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDF2498774.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2688..3491
                     /locus_tag="PC407_003366"
     CDS             2688..3491
                     /locus_tag="PC407_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2498775.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGIEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3734..4375
                     /locus_tag="PC407_003367"
     CDS             3734..4375
                     /locus_tag="PC407_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDF2498776.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1983 a          462 c          935 g         1551 t
ORIGIN      
        1 ctataaaaat ttgtaataaa gtgaaaaaac tagcttttca tgaaataata ttttataatt
       61 taagattgtc tttttaataa agattaacaa aaaaaatact ataattagtg gtataatagt
      121 tagagttgaa gattttaaat aaaggtaggt gtaattaagt tggaaatata cttagacaat
      181 agcgcaacta caaaacctta tcaagaagtt atagataaaa tggtttatgc acttaatacg
      241 gaatatggaa atccttcttc tgtccataga aaaggtgtag aagttgaaaa agctataaaa
      301 gaagtaagac aagatatagc taaatctcta ggagcgaaag aaaaggagat ttattttaca
      361 tcaggtggta cagagtgtaa taatactata ataagaggta ttactagttt aaataaaaaa
      421 agaaaaaatc acataatatc tactaacata gaacatcctt ctgttttaaa tacattaaaa
      481 gatttagaag aagatggttt tgaagtaact tatttagagg tcggaaaaga tgggaaaata
      541 aatatagagg atttaaaaaa tgcgataaag tcaactacat gcttagtaag catgatgcat
      601 gttaacaatg aaataggaac tatacagcca ataggtgaag ttggaaagta tttgaagggt
      661 ttaaaagaaa aaatttattt ccatgttgat gctattcagt catatggaaa aattaatttt
      721 agaccatcta aatacaatat agattttatg agtgtaagtg cacataaatt tcatggtcca
      781 aagggaattg gatttatgta tataaaagaa aataatcgcc ttaagcctat gttaacaggt
      841 ggtggtcagg aaattggaat taggtcggga acagaaaatg tacctggaat atatggtctt
      901 ggagaagctg ttagaatact aaacaaagat ttagatgttg ttatttcgaa agtaaataac
      961 ttaaaaaata tattaaaaaa tgaaataatt gataatatag aagatataaa aatcaattct
     1021 ccagaagatg gagtatgtca tatattaaat gtatcattta gagggacaaa aggagaagtt
     1081 ttacttcatt acctagagca aaaaggtatt tatgtatcaa ctggctctgc atgttcttct
     1141 aaaaagaaag gtagttacgt attaaatgct ataggtctta caaatgaaga aataaatgga
     1201 acaataagat ttagtttatc tgatatgaat acagaagaag aaatgttaga agctgtgaaa
     1261 gttttgaaag aatcaatttg tgacttaagg tcaataatga aaagaaaata aagaatactg
     1321 tatagatagt gataataaat tagtttaaga ggtgaaatgg tgtataatat attaatagta
     1381 aagtatggag aaattggagt aaaaggaaag aatagatata tatttgagaa taggcttata
     1441 agaaatataa gaaatatgtt aaaaccaatt ggaagattta atgtatataa agagtatgga
     1501 agaatatatg ttgatttaga agactatgat tatgaagaag taattgaaga agttagaaaa
     1561 gtatttggga tagtgggtgt gtgccctgga gtaagagcta aaaaagatta tgatacatta
     1621 aaagaaatag ctttaaaaat gttagaagaa aaaatagagg ctggatataa aacttttaag
     1681 gtagagtcaa gaagaggaga taagtctttc gggttgactt ctcaagaaat gagtatggat
     1741 ataggtggat acttattatc aaaggtagga gataggatta atgttgatgt aagaaatcca
     1801 gaagttaaaa taaagtgcga atacagagaa tttcatacta tggtctatag tgatactata
     1861 cctggatatg gtggattgcc acttggaacg aatggtagag ctatgtctct tttatcagga
     1921 ggtatagata gtccagtagc tacatggatg gttgcaaaaa gaggtatgga ggtagaggca
     1981 gttcacttcc atagttatcc atttacaagt gaaaggtcac aggaaaaagt taaggattta
     2041 gctaaaatac tggcaaaata ctgtggtaga gtaagacttc ataaagttaa catattagaa
     2101 atacaaaaag caattggtga gaattgtaat gaagaagaag ctactattct ttcaagaaga
     2161 tttatgatga ggatagcaca gagactttct gaaaaaagac attgtgatgc tttaattaca
     2221 ggtgaaagta ttgggcaggt tgcatctcaa actatacaag ggcttacttg tactaatgcc
     2281 gttgtagact taccagtgtt tagaccactt atagcaatgg ataaatcaga tatagttgat
     2341 atagccaaaa aaatagggac ttttgaaaca tctatagtgc ctgaagaaga ttgttgtagt
     2401 gtattttctc ctagaaagcc tgtcactaaa cctagattag agaaaataga aaaatctgaa
     2461 acagcacttg atattgaaaa attagtacag gatgctattg acaaaataga ggtagaggat
     2521 attgagtttt agcatacaag ttatttaatt actaaattga atattgagta tattagtagt
     2581 tctgtaagta tattagtagt tctgttgaaa ggatttatgt agacaattta ttaatatata
     2641 gtatttaaaa ataagtacta taattttatt taatgggaat gattattatg aataaaaagg
     2701 gttttacatt aattgaattg ttggtagtta tatctataat aggaatttta gttatagtag
     2761 ctgttccagc gttatttaga aatatagaaa aaagtaaggc agttacatgt ctttctaata
     2821 gagaaaatat aaagactcaa attgttattg caatggctga ggaatcaagt aaagacaaga
     2881 atgaagtcat aaaagaggta ttagaaaaca aagatggtaa gtactttgaa acagaaccaa
     2941 agtgtaagtc aggtggaata tattcagcaa cgtttgatga tggttatgat ggaataactg
     3001 gaatagaaag cattgcaaaa gtgtatgtta cttgtacaaa acatccagat ggtattgaaa
     3061 tggctaggga tatacatcaa agtatgaaag atttgattgc atcatttgca caagaccctt
     3121 ctataatacc aggagcttca aagggcaatg atgattttag aaaatattta ttagacaata
     3181 aatataaaaa tgggtggcct acaattccag atgaatttaa ggcaaaatat ggattaagta
     3241 aggatacact atatatacaa ccatatgcat ataatcctac taaatctgat gctactgtag
     3301 ttgtatttgc aaataataag actggaggta attggtatac ttccctagtt tacgattatg
     3361 atgaaggtag atggtataaa ggtaaaaatg gtatttctgt tgcaggtagg tcatgggatg
     3421 ttgacacaga tagtgttaag tctgtaaaaa cagagattca ttctaaagag ggatggggtc
     3481 ctttaaatta atatatcatg ttttattaat ctggtattga ttaaactata gaaaagagca
     3541 gtttctatag atgacgaata ccagattttc atttatatgt ataattaaga tatattaaaa
     3601 gtatttatat ttttaagtct tcacatacaa gctaaagata attataataa tattgtaaag
     3661 aaatttaatt ataagattaa ttttctgaag atttagtatt tgtactaaga aatacataaa
     3721 aaggggaata gatatgaata ataattttca agacaaccaa acaataaatt taatacaaag
     3781 tcgtagaagt atacgaaaat ttacaacaga gcaaattagt gatgagcaag taaacacatt
     3841 attacactgt gcatttgcag caccttcagg atgtaataaa cagccttggc acataactgt
     3901 tgtgcaagac cagaaacttt taaaagagat tagtgatgat actttatcta ggattcatga
     3961 agtgagtaat gttgagataa ataaaaactt taagttattt tatggtgctc caaccgtttt
     4021 atttatttct tatgatgaaa gtagttcatg ggctccatat gatataggaa tacttacagg
     4081 gaatataaca acagctgcac aagcactagg gcttggaagt tgtataatag gaatggttag
     4141 aggtttattt acacctgttg aacaagggga tattgaaggt cttgtgagtg tattggataa
     4201 agaagatgta aaggaatcag agtcaataaa gatgaagttt gatacaaata aaaaatacag
     4261 agaactactt gatataccag aagggtattc tgtaccattt ggtattgctg taggtattcc
     4321 agatggaaat ttaccaaatg ctagagaggt agtttacaaa gtttccagag tttaggataa
     4381 agtattgcaa gaaccaatca gttacttgaa gaattaaacc agtaatatac aaataaattg
     4441 atggaagagg aagctattta aaaagtagtt tagatttttc attgtaaaat aattaaataa
     4501 agaataaggt gataagtatt ttatttatta tcttattctt ttttattttt gttttgtaga
     4561 atgataaaaa taaggattaa gtgtaataaa atagaaaaca agtatgttaa ttttaagtat
     4621 atgctttaaa atttatccaa gttaaaaatt taagatatct tttcagtata atggaaaaaa
     4681 tgtcaaaata ttgaaatagg gaaaaaatag tgatataata aaaaaaatag agaaaaaaag
     4741 taacccttag ttgtaaaaaa gttactattt attgaaaatt tagatacttt tctaaaaatt
     4801 atctatgtta atagttaaat ttatgaacat tttttgtttt tatgttagga tatttcttag
     4861 aaatgaaata cttcttaatt tgatttgata tgtatatttt tatagctatt ttattataaa
     4921 aaaaaagaac c
//
LOCUS       DAMJVX010000225         4909 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMJVX010000225 DAMJVX010000000
VERSION     DAMJVX010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.231"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            167..805
                     /locus_tag="PC407_003368"
     CDS             167..805
                     /locus_tag="PC407_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2498777.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            812..1654
                     /locus_tag="PC407_003369"
     CDS             812..1654
                     /locus_tag="PC407_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDF2498778.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            1715..4342
                     /locus_tag="PC407_003370"
     CDS             1715..4342
                     /locus_tag="PC407_003370"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2498779.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            4556..4801
                     /locus_tag="PC407_003371"
     CDS             4556..4801
                     /locus_tag="PC407_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2498780.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
BASE COUNT         1970 a          555 c          974 g         1410 t
ORIGIN      
        1 taaagaaata tcccccatta atatataata aatatcttta agcgagagaa cttagtaaaa
       61 aatatatcgt attcaataaa aaatctgttt acagaatggt atatatagtt atataatata
      121 ctatataaga tgcgaaaaga taaaaagtat agcacaatga aagaaggtga ttattattca
      181 gcttaatgaa agacaactaa aaattataga tatagttaag gaaaatgaac caataacgag
      241 tgaaagtatt gcctccagct tgaatgtgac tcgtgctaca cttagatctg atttagctat
      301 attaacaatg acaggaatat tagacgcaag accaaaagtt ggatattttt attcaggagt
      361 tagtgaaata aatttaattg gtaaaagtgt aaaagaaaaa acagtagagg atataatgag
      421 catgcctgta ctagctaaga aggatgaaag tatatatgat gtaatagtca ctatgttttt
      481 atctgatgta gggagtatag ttattataga tgagaatgaa gagttatgtg gagttgtttc
      541 cagaaaagac ttgctaaaag caactatagg aggttctgat ataaataaaa tgccaatagg
      601 tatgataatg acaagaactc caaatgtagt cactttaact aaaggagcaa gtgttttatt
      661 agcttcaaga aaaataatag aacatgaagt agattcgatt ccgattgttg aatataaggg
      721 agaagacaaa aatcatatga gagttgttgg gagaatatca aaaaccaaca taactaagct
      781 attcttagaa atagttgaca attaaggagg tatgctggtg aaaaacctaa ttatatatgc
      841 agtatcagat tcagtagggg agactgcaca acaagttgca aaggcatgta tgtctcagtt
      901 ttatgtgaat gaaacttatg aaataaaaag atttccatac atgattaata aaggagtact
      961 acttgaaact ttggagaatg caaaagctga aaacgcttta attgtatata cattagttga
     1021 tgaagaatta tgcagtattg ttgaaagata ttgcgaaaga gaaggtctta gttgtataga
     1081 tttaatgaca gatatactaa gagaaataag caagagaaca ggaagaaaac ctaaaagaga
     1141 agctggtata attagaaaat tagatgaatc ttactttaag agagttgaag ctatagaatt
     1201 tgcagtgaaa tatgatgatg gaaaagaccc tagaggtgtt cttcaagcag acataatttt
     1261 agtgggaata tcaagaacat ctaaaactcc tttgagtatg tatttagcta ataaaaatat
     1321 aaaagtagct aatgttccat tagttccaga aataccaatt cctaaagaag tgtttgagat
     1381 agatactaaa aagataatag gtcttactaa ttcacctgaa aagctgaatg agataagaac
     1441 ccaaagatta aaagctttag gattgtccag taaagcaaac tacgctaatt tagaaagaat
     1501 acttcaggag ttagattatt cagaagagat aatgaaaaga attggatgcc cagtaataaa
     1561 cgtttcaaat aaggcaatag aagaaacagc aggtataatt ttagacataa tgaaagaaaa
     1621 tggtctaaag atatacaaag aaatagaaat ttaataatgt ctattaataa aaatgatata
     1681 aattaaaata aaaagattaa tgggggattt aacaatggaa actaagtatg tttatagttt
     1741 tggtgaagga agcaaagaca tgaaatcttt actaggaggt aaaggtgcca atttagcaga
     1801 aatgactaag ataggcttac ctgtccctcc tggattcact ataacaacag aagcatgtaa
     1861 tgattattat gttaacaatg aaagtataag agcagagata ataaaagaaa tagaagagca
     1921 tttagctact ttagaaaaag acttaaataa aacattagga tgcaataaga atccattatt
     1981 agtatcagtt cgttctggtg cagtattctc tatgccagga atgatggata caattcttaa
     2041 cctaggtctt aatgacaata gtgttgttgg cttagcagag gctacacaaa atgaaagatt
     2101 tgcatatgat agttacagaa gattcataca aatgttttct gatgttgcta tggaagttcc
     2161 aaaatataaa tttgaaaatg tattagacag agtaaaagaa gcaaagggat acacagttga
     2221 tacagaactt acaactgatg atttaaaaga aatagtaaaa gaatttaaag caatttataa
     2281 aaaagaaata aaaaatgatt tcccacaaga cccaaaagag caattaatgc ttgcaataga
     2341 ggctgtattt agatcatgga ataatcctcg tgcaatcgta taccgtaaat tgaatgatat
     2401 agctcataac ttaggaacag cagtaaatat tcaatcaatg gtatttggta atatgggaga
     2461 aactagtggt acaggtgttg catttactag aaatccagct acaggagaaa ataaattatt
     2521 tggtgaattt ttaatgaatg cacaaggtga agacgttgtt gcaggtataa gaactcctca
     2581 aaatatatca actcttgcag atgtaatgcc agctgtattc gatgaatttg taaaaattac
     2641 tcatatactt gaaggtcatt ataaagatat gcaagatata gagtttacta tagaaaatga
     2701 aagattatat atactacaaa ctagaaatgg taagagaaca gcagcagcag caataaatgt
     2761 agcggttgat ttagttgaag ctggaataat tgatgaaaaa gaagctatca tgagaattga
     2821 gcctaatcaa ttagaccaat tattacatcc aaaatttgaa gataaggcat taaaagaagc
     2881 caaggttata gcaaaaggtt taccagcatc accaggtgca gcaagtggta aagtttattt
     2941 caatgctgat gatgtagtaa aagcaaatga aaaaggagaa aaagtagttt tagttagatt
     3001 agagacatct ccagaagata tagaaggaat ggttaaagca gaaggaatcc ttacagctag
     3061 aggtggaatg acatcacatg cagcagttgt tgctagaggt atgggtaaat gttgtgttgc
     3121 tggatgtgga gaaataaaag ttgatgagtt taataaagaa ataagagcac ttgatgatgt
     3181 agttataaaa gaaggtgaat acatttctat agatggttct acaggaaatg tgtacctagg
     3241 agatgttaag aaaacagaag tatctttaac tggaaacttt gaaaaattaa tgaactgggt
     3301 agacaaacac aaatgtatga tggttagaac taatgcagat aatcctagag atgctcgtgc
     3361 agctatagag tttggtgcag aaggtatagg tctatgtaga actgagcata tgttctttga
     3421 tgaagctaga ttgccagcag ttagagaaat gatattatct aatacagtag aacaaagaga
     3481 aaaagctctt gaaaaaatat taccaatgca aagagaagat tttgtagagt tatttaaagt
     3541 tatggatgga aaaccagtta atataagatt actagaccca ccactacatg aatttttgcc
     3601 tcatgatgat gagactatag aagaactttc aaaatctatg ggaataaaag taagtgatat
     3661 taagaagaga atagttgatt tagatgagtt taacccaatg cttggacaca gaggttgtag
     3721 acttgcaata acttatcctg aaatatgtgt aatgcaatct aaagctataa tacaaggtgc
     3781 tatagaagca atcaaagcag gtgttaaagt ttctccagag ataatggttc cattagttgg
     3841 agaagtaaat gagttaaaaa taataagaaa aatgatagta gaaactgttg acgctataat
     3901 aaaagaagaa ggtatagaag ttccttatac tgtaggtact atgatagaaa tacctagagc
     3961 ttgtttaact gctgatgaaa tagcacaaga agcagacttc ttcagttttg gtactaatga
     4021 cttaactcaa atggcatttg gatattcaag agatgatgct ggtaaattct taggtcaata
     4081 tgtagatgaa gaaatattag aaaaagaccc attccaagta ctagaccaaa atggtgttgg
     4141 taagttagtt aagatgggag ctaaattagg tagagaagtt aaaccagaat taaaactagg
     4201 aatatgtgga gagcatggag gagaaccatc ttcagtagag ttctgttata gtgtaggact
     4261 taactatgta tcttgttcac catttagagt gccaattgct agacttgcag cagctcaagc
     4321 atctataaaa aatcctagat aataatagac tgcaaaatat gtaactaaat aaaaataaaa
     4381 tcccctattg ttcaatgtat caaattaagt atttgtaaat tgaatatagg ggatttttat
     4441 tataaaaatt taacttatac agttgtaagt gaaacgtatt taattatatt gcatatatta
     4501 ttgtagagag aaatttcaac tatcaattaa attgagttta ctggagggga taaagttgac
     4561 aaagtgtcct aaatgtggaa aggaagtatc atcacgtcca ggtagtatat gcccacgatg
     4621 tggatttaaa gtagcagatg agagtctaaa aataagatgt ccagaaccta gttgtagggc
     4681 tttggtatct aggaaactgg attattgtcc taaatgtgga tgcaaactta gaggatataa
     4741 tatttctgaa tttgttaaca tggtaagatg taaaatagat gagacatttg ataaaaaata
     4801 attaaattta gagttaagct tttgagggtg tctcaaaatg aactttttag ttcagaaggc
     4861 actctttttg tatggaatca aatatatttt tataatttaa acaataaaa
//
LOCUS       DAMJVX010000226         4867 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMJVX010000226 DAMJVX010000000
VERSION     DAMJVX010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.232"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            53..856
                     /locus_tag="PC407_003372"
     CDS             53..856
                     /locus_tag="PC407_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498781.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            868..1998
                     /locus_tag="PC407_003373"
     CDS             868..1998
                     /locus_tag="PC407_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2498782.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLRESIDEPIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEGFVKSYFESYSLIRG"
     gene            2002..3411
                     /locus_tag="PC407_003374"
     CDS             2002..3411
                     /locus_tag="PC407_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498783.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDMDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDVEIDYSP
                     ERTHDIWDESNEIDKNTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            3622..4131
                     /locus_tag="PC407_003375"
     CDS             3622..4131
                     /locus_tag="PC407_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2498784.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLSEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         2026 a          414 c          850 g         1577 t
ORIGIN      
        1 attattataa ttaatataga tataaaaata attatagaaa gaaaggcaca ttatggaaaa
       61 attgagagtt attgaaatga taaaaaataa tcccaatatt atagctacaa tagataatcc
      121 aactgatgaa atgaaattgt tagctattaa agaaaatggg cttgtactag aatatataaa
      181 taatccaacc agagagatgc aagaactagc aatagataat aatattagag caattaaatt
      241 tatagataat ccaacagaag atatgatgat aaaagcagta aatgagggat ggagtatact
      301 ggattatatc aaaaacccaa ctgataaagt tattgaaatg gctataaatc aagcaggatg
      361 ggcaattaaa tatgctaaaa atccaagtga agaattacaa ctgttagcag taagaaaaaa
      421 ttatgattcg ataaagttta taaaacaacc ttatgaaagt gtacaagagg aggcagtaag
      481 aataagttat gatgccttga gatatataga ttcacctagt tataatgcag aacttatagc
      541 tataaaaaat aatgaagcag ctattagctt tataactgat ttagataaaa ataaaatgtt
      601 ggaattttta aaagtgaata ttttagtgat taagtacatt ctgaaaaaag tatctaaaga
      661 tgaattagag agtgtattaa aagaagtatt atccaaagaa gatgttgagg aaaagtatat
      721 tagagatttt ttgaattgta gtgttataga tagaaatagc gaaagtatac caatagataa
      781 aattatgttt atttataaat atggaagtaa gacagcaaaa aaaatagctg tagatgagaa
      841 attaaagatt atgtaggaga gagaaaaatg aattttatag acacattaaa aagcgttgac
      901 ttaatattat ccactggaga agtaccttta atagtaggtg aaagtggaat aggaaaaaca
      961 gctttagcaa ataagcttgc caaagaaaat gattggagtt taattgttat tgatggaaac
     1021 ttacttaaag agggtgaaat aggtggtctt ccaactatag aatcttatgt aggagttaat
     1081 tctaatggat ataagactga aaagaaaaca acagtgtatg ctgttcataa caaattaaga
     1141 gaaattgatg aagagatatc taaaagtaag acagttcttt tatttataga tgagataaat
     1201 cgttgtgagc atacagtaca gcaagaactt atgaatttaa ttttaaatag agaaattaat
     1261 ggatataaac tgcatgatga tgtaaagatt ttagcagcaa tgaatccatc aagtaaatat
     1321 ggttcagatt ttgattatca agtggtagat atggatgcag cccaagaaaa taggtttgtt
     1381 tggttaaata tggagagtga ccatacacaa tggataaaat gggcaataga tgagggtatt
     1441 gaaagaaaag ttatcgagtt tatttccact tttccagaat atctgcataa aataaatgaa
     1501 gatgatgtaa gagctactcc tagaagttat gagagagtat ctaaaattta taaagtttat
     1561 aaagaaaaaa ataattctat tcctagagca gtatttttaa atgttgtaaa gggtaatgta
     1621 ggtaaagtta ttgcagaaga atttataagt tttattgaat caaactctga accattaata
     1681 tcctatgaag atgttttttt aagagaatct attgatgagc ccattgtaga aagagtaaaa
     1741 aatgagagtc atacaagact ttatctatca gctatgaaca ttctaaaaga cttagaatta
     1801 aatattaaga atgataaata tgaatcaaac cactatatta atagatttat tgaattttta
     1861 aaaatgtatc ctgtagattt aatgataggt attatgaagg atattagaaa cagttatatt
     1921 gaagtatata aaaaagccat agaaaatgaa ggttttgtaa aatcatattt tgaatcttat
     1981 agcttaataa ggggataatg tatggaaaat tattttgata aacaagcaaa agagctttat
     2041 aataaagcaa gtgaaattat aaatactcat tctatgctaa aggcaaatcg tgctaatgag
     2101 aaatttgata tggatatacc acaagatttt aagtatgagt tttttagtct tgtagataaa
     2161 gtgaatttaa gtcttatgga agaagaagat aatttttatg gatatttttt gtttcagatg
     2221 tcaagagaaa taagatttga tataagtagt cctacaggcg tgaattttaa gggagctaaa
     2281 tatgttatat attttaatcc aataatcttt ttaacgcttg acataaagca aatggagact
     2341 acaattaaac atgaaattca tcatatatta tctatgcatt tgatgagagc aaaggagtta
     2401 aaaggaaagt atagtacatt ggcaattaat atggcaatgg atatagtagt aaataaattt
     2461 ctaaacaatt taccaccata tgcaactacc ttagagtggg taaatttaaa atattcctta
     2521 aaacttgaac catatgaacc ttttgaatat tatgtagaaa agattcagac tgaattagat
     2581 ttgatggaag tggatgaaga tggtgaggaa gatgatagtg ataaatatgg agatgtagaa
     2641 attgattata gccccgaaag aactcatgat atatgggatg aatccaatga gatagataaa
     2701 aataccctta gagaatttac tgaaaaattt attaacagtt ctcaaaaagg taaagttcct
     2761 gcttatttag aaagtatgat atcatcactt aaaaatagta agggtgaatt gccatggaat
     2821 ttatatctta atagaataat gggaacagtt gaaaataata aaaagaagac tataacaaga
     2881 agaaatagaa gacaaccaaa tcgtttagat ttgaggggag aacttagaag tcataaggca
     2941 gaaatagcag ttgcacttga tgtaagtgga agtattagtg atgaagaatt taaacaagct
     3001 attaaagaag ttcttagtat agtgaaaaac tataatcatg aaattacaat tatagaatgt
     3061 gataatgaaa ttagacgtgt atataaagtg agaactgaaa aagatataaa agatagaatt
     3121 aggataggtg gaggtactag attttctcca gtctttgagt atgctaataa taaaaagatt
     3181 aatttgttag tctactttac tgatggtaaa ggtgaaaata aactacaagt aatacctaga
     3241 gggtataaaa ttttatgggt tatttctgga agaggagata aaatttcatt aaaagaacct
     3301 tatggagcag ttaaaaaact tactaaggtt aaaacaaatg atgaaatatt agaaataaat
     3361 gatgtaagaa atgatgggta ctcaatgaat agccaagcac caattttata ggtattcaca
     3421 ggagataagc tgtattaatt tattttgata tgcttgttat aaaaatctaa aaaatacatt
     3481 aatcaatggt taatgtattt tttaatttta ataatataag ttttacttac atttaagtct
     3541 taaaatgtgt taaaatataa tatatagcag atagttttgg caagatggat ttagttgtca
     3601 aaactatttt ttttattaag gatggtgatg tttttgttta agattgatga ttatataatg
     3661 tatggtatga caggagtctg caaagtcatg gatataacaa atgaacgatt tacaaatgga
     3721 ataaaaaaag agtattatgt attgagtcct atttattcta ataatacagt aataaaaatt
     3781 cctgtagata atgaaaaagt accaatgaga aagttacttt ctaaaattaa tgtgctttca
     3841 ttaataaatg atattcctaa tatggataca tcatggatag ataatgaaaa attaagaagt
     3901 gagcaattta aaaaaatact cagaggtggt aaatgtgaag agttaataaa attagtaaga
     3961 agtatagata ataacagaga gtatgtaaaa tctattggta aaaaaacaca tcaagcagat
     4021 gataatataa tgaaagaagc agaaaggtta ttaagtgaag aatttgcaac tattttagat
     4081 atttctccta atgaagttag ttcatatatt tcaagtcata tacctcaata aagtgtttta
     4141 gtatccttta tttaatctat tttaagtatt atttatgtat gctggttttg gtaaaataaa
     4201 atatataatt aaattatata aaaaattaag aatgattgta ttgaagaatt acagaatagt
     4261 gcggagagcc cactccttta ggtgtgagat ggatatcact taaagattta aattgttatt
     4321 gtagatgttt ttaatataaa gcaagattaa taagtgtatt gtaagcttta agtgaaattg
     4381 atactataat gagttactta gattttaaaa tatataatat tataaaaaat ccttttaggt
     4441 tttgaatgaa taagatggat actatgttga atagaatcca tcttatttaa gttttattta
     4501 tgttgtttat ttgtaagcat tatttttgta catgaaaaca cagtgtaaaa atatgcttag
     4561 aattatgaag ttttattaaa aatatattcg acaaaataag actaaaaata ttcttaaact
     4621 tataactaag ataaatattg tatatttata gtgtagcctg aaaaattgtg gatgtacgaa
     4681 atttatttag tattttattg aaacaaagta gaagaagtac tatagtttat ctttgaatag
     4741 attgttattt tttagtgaaa aagttattag gttaatacat aaatataata gcttataaat
     4801 agtttaagca tgttaaaaaa gtagttatta gagatatata gttacattgc ttataagata
     4861 cttaaaa
//
LOCUS       DAMJVX010000227         4831 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.233, whole genome shotgun sequence.
ACCESSION   DAMJVX010000227 DAMJVX010000000
VERSION     DAMJVX010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.233"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            141..802
                     /locus_tag="PC407_003376"
                     /pseudo
     CDS             141..802
                     /locus_tag="PC407_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(1021..2058)
                     /locus_tag="PC407_003377"
     CDS             complement(1021..2058)
                     /locus_tag="PC407_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDF2498785.1"
                     /translation="MNKKLIKAKIFIWSILAISLSIVFISLLIGTIISHSFNFPFNTL
                     NPKYNYDNYIVAKNESFSDKINDINVDWISGEVRIKRSDSNKIKLIQKIPSNFSKKKL
                     AKIMVDGNSLSIYDNSTKKFFIGINLPKPTILELYLPNKLYDKITLYTTSADIYSTYI
                     RSNTVEANTISGAIGLSGNINKINLDTTSGHIDITKLNSKNATLKSTSGEIQLSGKID
                     VLNLETTNGNINLKDIYNKNLTCSSVSGKMNLSGVFSDIKAESTSSNIKINSSKMLSS
                     LYCETTSANVDLSIPDNPGFTLNFDKVSGVLDSDFELNRHSDLYTYKNGITNLYLSTT
                     NGNLTIIKNNH"
     gene            complement(2051..2737)
                     /locus_tag="PC407_003378"
     CDS             complement(2051..2737)
                     /locus_tag="PC407_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498786.1"
                     /translation="MLDKIRTYIDSSFTGVNETKKVKELKDELFENLKEKYNDQIQDG
                     KTEQEAYNSVISGIGDLSELIESVKEPYSLPSELIEEKKKRALRASIAVALFIISPFL
                     FVVSVVSFGIEPTIAILLMLILIALGVGLLVYNQMTRTNYALYNDNLSNKFKEFEDFE
                     ENYNHINPVYKSFMNAYWIIVVSIYLITSILFNIWSFSWILFIIGAAGVNIVKGIIQL
                     KGYKEEDKYE"
     gene            complement(2749..3069)
                     /locus_tag="PC407_003379"
     CDS             complement(2749..3069)
                     /locus_tag="PC407_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2498787.1"
                     /translation="MIIASDMIRGHTETIILKHLLEKDSYGYEINKSILEKTKGLYEL
                     KEATLYTAFRRLEQSGAITSYWGDEVTGARRKYYSITNIGKEKYIKSREEWNNTKALI
                     DILI"
     gene            3430..4770
                     /locus_tag="PC407_003380"
     CDS             3430..4770
                     /locus_tag="PC407_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2498788.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLFLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT         1783 a          532 c          720 g         1796 t
ORIGIN      
        1 taataacaat tcaaaataaa taacttatgt aagaaccgta ggaactacgg ggatagcctg
       61 tggagaatta gtaagacata tgttagtatg caaaattcta tgaagcagga accctgtgac
      121 tttagtcatg ggaggttcag attttggctg aagatgaaga aaataatcat ataaatattg
      181 agatgcaaag agcttttgag aacgattatt tggaaagagc tgaatattat cttagtcgtg
      241 tacatggtag aaagttagaa gaaggtaaag agtataaaga aattggaaaa actattggaa
      301 tacatatact aaatcatgta aaatataatc acatagaaga ttatgtaaat tgcctgagat
      361 taacaatgga cgagcatcca gatatttatt ctagtaaaac tgcattatat tttatagagc
      421 ttccaaaaat tcataaatct gactatatag taaatagaat tatgctatgg ggaaaactta
      481 ttgataatcc ttctagctca gatgtaagag ttatagctaa gaatgattct attattaaaa
      541 aagcacttga tagattaaaa gaattaggta gcaatgaaga atatttatta aatctgaaaa
      601 gaggtgctta tatcatgaat aaaagtagaa attttaaaga agagattgaa agagagacag
      661 caattagagt tgcaaaagaa aaagattata aaataattat aatgttgaaa aaaatggttt
      721 taaattagat gacatattga ataaatttaa tgattttgac ttaagtgaag atgagataaa
      781 agaagtatat gaaaataatt gatgataaat aaaaagtgaa cttatataag tttgctgaat
      841 ctgagcctgg aaatatttat gtgtattttt taatgaataa aaatttattt taaaaacaat
      901 aattagaggt acagttaatt atatttttat gatactgtac ttctagtttt ttctattgta
      961 gatttattat ttactaataa atctacaata gaagtgcttt tgttgtaaga atcattttat
     1021 ctaatgattg ttttttataa tagttaaatt tccatttgta gtgcttaaat ataaatttgt
     1081 aataccattt ttataagtgt ataaatcact atgacgattc aactcaaagt cactgtccaa
     1141 aacaccagaa accttatcaa aatttaaagt gaaacctgga ttatcaggaa tagacaaatc
     1201 gacattggca gaagtagttt cacagtacag agaagacaac attttgctag aattgatttt
     1261 tatattactg ctagttgact cagctttaat gtcagaaaaa actccagata agttcatttt
     1321 tccagaaact gaactacaag taagattttt attgtatata tcttttaaat ttatattgcc
     1381 attagttgtt tctaagttta aaacatcaat tttcccagaa agctgtattt caccagatgt
     1441 tgatttgagt gtagcatttt tactatttag ttttgttata tcaatgtgac cactagttgt
     1501 gtctaaattt attttattaa tattaccaga gagacctatt gcacctgaaa ttgtgttagc
     1561 ttcaactgta ttacttctta tgtaagtgct atatatatct gctgaagtag tatataaggt
     1621 tattttatca taaagcttat tgggcaaata tagttcaagt attgttggtt ttggaagatt
     1681 aattccaatg aagaattttt tagttgaatt atcatatata gaaagagaat ttccgtcaac
     1741 cattattttt gctagttttt tctttgagaa attactaggg attttttgaa ttaatttgat
     1801 tttattagaa tcacttctct taattctaac ttcacctgaa atccaatcaa cattaatgtc
     1861 atttatttta tctgaaaaac tttcattttt agccactata tagttatcat aattgtattt
     1921 aggattaaga gtattaaaag ggaaattaaa agaatgtgaa attatagttc ctataaggag
     1981 tgagataaat acaatagaaa gtgatattgc caggatactc cagatgaaaa tctttgcctt
     2041 tataagcttt ttattcatat ttatcctcct ccttgtagcc ttttagttgt ataattcctt
     2101 tgacaatgtt gacaccagca gcacctatta taaatagtat ccacgaaaag gaccaaatat
     2161 taaatagtat gcttgtgatt agatatatag aaactactat aatccaatat gcattcataa
     2221 atgatttata aactggattt atatgattat aattttcctc aaaatcctca aattctttga
     2281 atttatttga taaattatca ttgtatagag catagtttgt ccttgtcatt tgattataga
     2341 ctaataaacc tactccaagt gctattaata tgagcattaa aagaatagct atagttggtt
     2401 ctatgccaaa acttacaact gagactacaa ataggaaagg gcttattata aaaagagcaa
     2461 ctgctattga tgctcttaag gctcttttct ttttttcttc aattaattca gaaggtagag
     2521 aatatggttc cttaacactt tcaattaact cagataaatc accaattcct gagattactg
     2581 agttatatgc ttcttgttct gttttaccat cttgtatttg gtcgttatac ttttctttta
     2641 gattttcaaa taattcatct ttaagttctt tgactttttt tgtctcatta actcctgtaa
     2701 agctactgtc aatataagta cgtattttgt ctaacatatt ttcacctcct aaattaatat
     2761 atctatcaat gcttttgtat tattccattc ttcacggctt tttatatatt tctctttacc
     2821 tatgtttgtt attgaataat actttcttct agctccagta acttcatcac cccaataaga
     2881 ggttattgca ccagattgtt caagtctacg aaatgctgta tacaaagtag cttctttcaa
     2941 ctcatacaat cctttggttt tttcaagtat agatttatta atctcataac cataactgtc
     3001 tttttcaagt aaatgtttta agataattgt ttctgtatga cctcttatca tgtcagatgc
     3061 gattatcatg aaaatcacct ccaattaaat atataatact ttgatatact tcgcctgtca
     3121 aggtatatca tgagaaaaga cattttattg taatatttat aaggttttat atagaaataa
     3181 tgtaaaaact ttataaataa tatattttat acctagttat agataacttt atatagttat
     3241 tattaaaaaa agcaataaaa agtttaattc aatttagcaa atttagtgat ataattaaaa
     3301 aagaaaagaa atatggactt atttggaata attttgacaa tctatttact acacgtgtag
     3361 agtcgtatat aattaaatta tacataaaat ataaaaaaat aatttagtaa tacgttagga
     3421 gaaagtatta tgataaagtc aagcttattt tggaatattt tcattgcgat atcctttatg
     3481 atagaatggt ttctttgtaa gaaaatgcta gattatacaa gtgagaaaag gatttctaac
     3541 aaaaaaataa acttttgtat ggtaggtatt attgtttttg ctgacataat atattttttt
     3601 gatgtacctc caaatataag agtaattata tctatgataa ttactattgc attttataaa
     3661 atctcatatg atgtgggatt tataaagtct gtattaatat ccttaattta ttggatgtta
     3721 ctaatcgcca cagatgcttt gagtatatct ttaacagttt ggcttaattc tataaatgac
     3781 atgagtagta tggggacggg agatatatac agaattgagt ctataatatt gggaaagata
     3841 cttttgattg tgtctttgtt tttatataga gccttaaaga taaaacttaa tatagaaata
     3901 aataaaagga cattatttta tatagttatt cctatagtcg ctaatatagc atcttttttt
     3961 gtaatttttg aatacatatt taatccaggg tctggtagct taatacataa tttccaattc
     4021 gttatgattt ctgttgtatt gcttatatca agtatcactt tagttttaat tgctaaaaaa
     4081 ttacagcaat ataatacttt agttgtagaa aacaatatga ttaaaaatac tttgaagtat
     4141 gaaaatgagt attatgaaga tatagaaaag aaatatttaa aaactagatt actaagtcat
     4201 gatatgaaaa atcacatact gtgtataaat gctatgatta aaaaaggtat tgatgttact
     4261 tcttatttaa ataatttgca ggtagaaatt cagagtaatg acttgttatt taatactggt
     4321 aattttatat tagatgctat attaagtgaa aagaaagaag tttgtgataa gaatgatata
     4381 gaactaaaaa taggaataaa ttttagttgt tgtgaagctg ttgaattagt agatatatgt
     4441 catatatttt caaatatttt agacaatgca attgaggcat gtaataaaat tgatagtgga
     4501 aatagagtta tttgtgttaa aggtgatgtt atgcaaaatt tttatttaat aagagttgaa
     4561 aatactaaga tgaataagat tattactaaa aatggagata ttttgacaga caaaaaaggt
     4621 tctcttcatg gacttggaat aaaaagtgta aaaaactctg taaataagta tggtggtaat
     4681 gttgtaattg aatatgatga taaaaaattt gtagttaaaa ttgctattcc acttagaaat
     4741 gtgaagaaag cagttaatca tgtgatttaa atgtatataa atttgatgtg tagaaattta
     4801 atatataaga attaatgtat aaaagtgatg t
//
LOCUS       DAMJVX010000228         4817 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMJVX010000228 DAMJVX010000000
VERSION     DAMJVX010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.234"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            244..1338
                     /locus_tag="PC407_003381"
     CDS             244..1338
                     /locus_tag="PC407_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498789.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            1564..1980
                     /locus_tag="PC407_003382"
     CDS             1564..1980
                     /locus_tag="PC407_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498790.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(2162..2731)
                     /locus_tag="PC407_003383"
     CDS             complement(2162..2731)
                     /locus_tag="PC407_003383"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDF2498791.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            2885..4042
                     /locus_tag="PC407_003384"
     CDS             2885..4042
                     /locus_tag="PC407_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDF2498792.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            4229..>4817
                     /locus_tag="PC407_003385"
     CDS             4229..>4817
                     /locus_tag="PC407_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2498793.1"
                     /translation="MFNKNEILKKLIEEYDVKTTTDIQDMLKDLFASTIHQMLEAELD
                     DHLGYDRYDNKNKNTKNSRNRYRNKNVKSDFGEITLNIPRDRNGDFEPKVIRNYENDI
                     SGIENQVIGMYSKGMSTRDISSHLKSIYGIDISHSLIAKITDRVLPLAHEWQSRALDM
                     VYPIVFMGAIHYKVRSEGRIINKAAYIAIGVNLEGI"
BASE COUNT         1818 a          516 c          855 g         1628 t
ORIGIN      
        1 aaaatatgac taatatataa tctatagaga aactaatttt atatataatg actacttaga
       61 taaaagaatc taaaaaaatt atgagaggag gagtagggta taaaatcaga ttataagttt
      121 taagttatta tactaaaaag ctataaaaca gttatcatgt attttatgtt aaaggaaatt
      181 gtaggtagtt tatcgaatta tttaatcaga agaaaaaatt tcacatgggg aggtaactga
      241 gatatgtcag aaaaagtaaa tgttaaaaca gtcatcagtt tcgcaggtgc gtatgtagca
      301 acagtaatcg gttctggatt tgcaacagga caggaaataa tgcaattctt ctctttttat
      361 ggttttgcag gtattattgg tgggataatt tcaatggtat tattctcatg gatgggtgct
      421 tcagttatgt caaaaggtaa agagcttcaa cttaaagaac ctatcaaaat ttatcgtgtt
      481 tattgtggta agtatttagg gatattcttt gaatggtttg gacctttatt cttatttggt
      541 gttttcgtag taatgatttc aggagcagga gcaactttaa ctgaatacta tggattaaat
      601 ccatttgtag gtagagtagg tatggcagta gtagcattat taactgtttc actaggtctt
      661 gataaattat caaagatact aggtggaata gggcctataa taataatctt tacactttta
      721 gttggtggta taagtttagc taaaaacata ggtaatatag gagaagctgc acaagttctt
      781 agcactgtaa agatagctaa accagttcca aatgcctatc tatctggtgt aatatatact
      841 acttataaca caattgttgt tatggcattc ttaactggat taggagcaag tgctgctaac
      901 aaaaaagaag ctatttgtgg tggtatatta ggtggagtag cacttatggc tgcagctata
      961 atgatgcact tagctattct ttctgatata ggaaacttat attcaaaagc aataccatca
     1021 ttattcttag cagataagat ttcaccagta attggagttt tattctcaat aattcttata
     1081 ttaggtatat atacaacagc tgtaccttta ttatggtcag taactaatag atttgtagat
     1141 gataaacatc ctaagtttaa attaataact ttaatagcag ctatattagg tcttataggt
     1201 ggcttcctac catttgataa gctggttggt attttatatc catatactgg atatatgggc
     1261 gtcataatac tggtttgtgt tctttataga cagattacaa aaacatctgg atatcaagaa
     1321 ggaatcagtg acaaataagt attttttaat attgtaaact caagagctat atgaaaacaa
     1381 tgaaaatgtt ttcatatagc tctttttatt ttttagctcg tttattatga aataggaaaa
     1441 acatgttttt tagttacatt acatatattt ataattatgg ataaatatat actaatcaat
     1501 aatttagagt ctaagataag cttataaatc atatatttag aatatatggg aggagtgata
     1561 agattgaatg taaaatttaa tattaaaggt ataatttatg gagttatagc tcttgtattc
     1621 gttatagggg gatttatatt cataccaagt atgtttgtag agaaatctaa acccatagat
     1681 tatacagtat tacaaagaaa ttcaatacca gaaaagatat tagatgtgat ggataagtat
     1741 acaaatgaag aaagagcatt agcggctaaa atcgataaca aaatatacat tatagtgact
     1801 agaggaaata ataaatatgg tatagaaatg gacaaaatag aaagtgtggt tgaggaagga
     1861 aaggatgttt tgagagtcaa gataaagtat aaagataagg aagattcttt tccatacata
     1921 gttgttgaaa caaatatgag tgaacttcca gatagaatag aattaaatgc gacgaaataa
     1981 gaaggtattt tataattaaa taactgcata ttgtacaata aagtcatggt ttttattaat
     2041 gtaagtaaat tgtatatggt tcttattgtt gtaaaagcca tgacttaaaa tgcttataaa
     2101 gtaagatatt aacagtagta tttattacta tattaaatgg ggtctggaaa tacttaatgt
     2161 tctaattatt tggagtgata attctagttt cagtaactat gtagtctaat tgtgcatcat
     2221 gagtttcttt tggaatagaa tcgaatatct ggaagtcaaa tgctatgcca atagttatag
     2281 catcttttct taagttttct aaaaatctat cataaaatcc tccaccatat cctaatctat
     2341 agcaatgtat atcatatgct acagctggta caattacaac atctaaggcc ttaatatcaa
     2401 ttgtaggtga tttttcgtta ggttctctta ttccatatgc tccaattctc aatccctctt
     2461 ttaaatctgt tatttgagta gggattaatg tatgattttc ttttattgtg atgggacttg
     2521 atactatttt ctttagagac aaaagttttt tgattaaatt atctgtttga acttcattgt
     2581 taaaatttag ataaagcatt atattagttg catttcttat atcttccatt tgaagtaatt
     2641 tttcagttat taaatttgaa ttactaagta caaagtttgc actcttattt tttcttgatt
     2701 caataacttt ttttctaaat tcttttttca taaaataatt cctcccttaa tatgttataa
     2761 tgtgtacagc aattgtattt aatataaatt ttagtataat ttattgtaat aaagcaatat
     2821 atttgggtaa atgtaataaa aataacagct agtcatataa taataatagt ttatgtaaaa
     2881 ggagatgtat cttgtgattt caaagaagaa agctattttt ttaggggtga ttttagtaat
     2941 tataacagct atggttacat cagcatttca gttgacattg gggaataaag ttgtaatatc
     3001 gaaagaactc tatgaagact ataaaaaata tgataagttg ttaggactag aatctattat
     3061 aaaacaagat ttttataaaa aagtatcaga tactgatttg gtaaatggag cagcaaaagg
     3121 tctatttcta ggaacaaatg ataagtattc tggttattat actaaagatg aaatggagaa
     3181 tctaattaat gatagtgaag gttcttatgt tggtgtaggt atgtatatag gggcatcaaa
     3241 agatggtggc ttagtagtag ttcccatgaa agattctcca gcagagaaag caggtgtaaa
     3301 atcaggggat aaattagtaa aagtcaatgg aaaatctgtc tcatataaaa attcagatga
     3361 agctgttcgt atgatgaagg gtaaaaaagg gaaaacagtt gaactgacta tattaagaga
     3421 agataaacaa ttgaacttta aagtaaaaac agaccagata attgagaaga gtatagaaag
     3481 taaagtaatt gataatgatt taggatatat agaaattacc caatttatat caagtacata
     3541 tactgacttt gataaagctt taaaagaatt aaaagcaaaa aatatcaagg gattagtaat
     3601 agaccttaga aataatcctg gtggaatgct agatatatgt aaagaagttg ctgatgagtt
     3661 aattggtgaa ggaactatag tatatacaaa agataataag ggaaatactg agtacttgaa
     3721 atctgataaa gaaaaattgg gacttccaat agtagtgtta actaatggag aaagtgcttc
     3781 tgctgctgaa atattaacag ctgcaatcgt tgataataaa gaaggtattt ctgtagggac
     3841 tacaactttt ggtaaagggt tggtacaatc agttgtaaga ttgaaggatg gaacaggata
     3901 caagttaact actgctcaat attttacacc taatggagat tatatcaatg aaaaaggcat
     3961 aaagcctact attgaagaaa aagatgaaaa taaacagtta gatgtagcga ctaaatggct
     4021 tagagaacag attgataaat aaagtttaat ttttataaac ttatattgta aaataaagaa
     4081 ccttaataaa aggagtgtct caaaattaac tttttagttt atgaggcact cttttgtggg
     4141 tctttaaaat aaagtgtgta ttcttctaaa aagtagtgat aattaaaata ttaaatatca
     4201 ctatatataa ggaaggtgca cactttttat gtttaacaaa aacgaaatat taaaaaaact
     4261 tattgaagaa tatgatgtta aaacaactac agatattcaa gatatgctaa aagatttatt
     4321 tgcaagtaca attcatcaaa tgttagaagc ggagcttgac gaccatttag gttatgatag
     4381 atatgacaat aagaataaaa atactaaaaa ctctagaaat agatatagaa ataaaaatgt
     4441 taaatcagat tttggagaaa taactttaaa tattccaaga gatcgaaatg gagattttga
     4501 accaaaggta attagaaatt atgaaaatga tatatcagga atagagaacc aagtgattgg
     4561 aatgtactca aaaggtatgt ctactcgaga tatatcaagc caccttaaga gtatttacgg
     4621 aattgatata tctcattctc taatagctaa aattactgat agagttctac ctttagctca
     4681 tgaatggcaa agcagagctc ttgatatggt ttatccaatt gtatttatgg gtgctataca
     4741 ttataaagtt agaagtgaag gtagaataat aaacaaagct gcatatatag ccataggtgt
     4801 taacttagaa ggtataa
//
LOCUS       DAMJVX010000229         4754 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMJVX010000229 DAMJVX010000000
VERSION     DAMJVX010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.235"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            105..806
                     /locus_tag="PC407_003386"
     CDS             105..806
                     /locus_tag="PC407_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDF2498794.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            799..1122
                     /locus_tag="PC407_003387"
     CDS             799..1122
                     /locus_tag="PC407_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDF2498795.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(1328..3388)
                     /locus_tag="PC407_003388"
     CDS             complement(1328..3388)
                     /locus_tag="PC407_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF2498796.1"
                     /translation="MEKYRALKLVSLIIISISFPLTILLATIGLAEPKYNIQKTSTKS
                     GIFNISNDFYDSNIFDEGTVESLLSNFKISVDSQLTSEEKSKLQTKSKSIDLSSNDSE
                     YDKIMINKQASEEYLSYLKNIKVYVINTYTGVFYTNTSYGNLDDFKQKTKSYCNIEII
                     NNVGKSSYIKTINGEKFELNNLSMELNGVDEAFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVACLSLAIVCLLSIILYSRIKGEVLKRNNVFLLIYSKIPMEFNVILVF
                     ISIAILTMYALAVSYHIDVIVLVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYIKVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(3584..4276)
                     /locus_tag="PC407_003389"
     CDS             complement(3584..4276)
                     /locus_tag="PC407_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2498797.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
BASE COUNT         1588 a          716 c          454 g         1996 t
ORIGIN      
        1 attaatccaa atttattttt aattataatt gcaattatac aattaatcta tttttctgtt
       61 ttactaatac tttaatacat ttaatacaaa aggagtgatt tattgtggaa agaactcaac
      121 aattaaaaga aagctttatt gcttcaattc ctatattctt aggttatata cctataggta
      181 tagtaggagg aatactatta caaaaatctg gtctttcacc ttttcaaata gcactaatgg
      241 taactttagt atttggagga agttctcaat tcattgcagc ttcaatgatt tctactggtg
      301 caagtgtaac atctattgtt ttgactacat ttatagttaa cttaagacat tttctaatga
      361 gttctaattt gaacatgtac ataaaaaata aaagtccaaa atttatattg ccattttgtc
      421 atactataac agatgaaact tttgcagtta attatgaaaa atttactgct ggaaattggt
      481 ctgatagaaa tgctatttat cttaatttct tctgcttaat ttcatcagtt ttagctaatt
      541 ttattggagc atttttagga gaaagtatat ctatagacag ttctatttct ggcttcatat
      601 tgacttctat gtttatagct ttaatagttt ctcaaataaa aaataagatt tatattgtag
      661 tatttatatc atcaatcatt atttctgtta ttttatatgc attattcaaa agcaatttag
      721 taataatagt tgcatcaatt ttagcatctt taattggttt ctttatagaa gaatcttact
      781 gtaaaaagga ggaatcttat gaataataat tatatatttt ttattataat tggaatgtac
      841 attgttacat atctaccaag agtgcttcct atgttaattt tttcaaaaaa agagatgcct
      901 gattttttga aaaaaatcat gaaatttata cctgtagcaa tacttacttc cttaactgct
      961 aaagatgtat tttttaatgg agatagtcta tatttatcta tagctaaccc taaaattata
     1021 tcttttatga ttgtacttct tgttgcatat aagtttaaat ctattggtat ttctattatt
     1081 acaggagtga tatctatata tttatttgga attatcttgt gatatttgga taatgtttta
     1141 ttgtataagt tattttaaaa ctatgtgtac aattttaatt taagttgtta tattattgat
     1201 tttaaataaa aaacagagtg acttaaagtt actctgtttt tactcttcat tctctactgt
     1261 aaatattcta atatattata ataaaagctt tcgacagtaa aaaaaatata tatacttaaa
     1321 tactatctca ttccatattt gttatttctt cttcatattt ttgaaactct aaatttgctt
     1381 taaataaatc tccatctatt tctatataaa attttccacc ttgtaaagct actaaatctc
     1441 ttgcaatagc aagccctaat ccacttcctt cagtattcct tgattcatca gctcttttaa
     1501 atctttccat aatctcactt gggtcaaaat ttaattcata agaagatata tttttcatag
     1561 ttacctttac attttcacca tagtcatata catcaatata gactctagtt tcttctaagg
     1621 aatattttgc tatattagat aaaagatttt caaatactct atacattctt cttccatctg
     1681 cccatatata caccttatca tttggaacat ttaattttaa atataaatta tttttagata
     1741 atttttcttc aagttcacct atgctttgtc ttaatagttg cactaaatca atcttgctaa
     1801 tttgtaattc tatattgcca ctacttactc tactcgcttc aaataaatct tctatcaata
     1861 attttagtct ttttgacttt gactcaagta cctttatata atcatttata tactctggtt
     1921 gtattgattc ttctttttta attaaatcta cataatttat tatagaagta agtggagttt
     1981 tcaaatcatg agatacattt gttataagat cagacttcat tctttcactt tttacttgct
     2041 cttctatagc tctatcaaga ccttctccaa tattatttat attttcagct aaagacgtaa
     2101 aattatcatt cccagtaatt tttatcttat aatctaattc tccctttttt attctttctg
     2161 taccttctat tacgtaacta agatatgaaa gtcttttagt tatatatatt gtaaatccta
     2221 aaaaaataca tatagaaaac agtaatccca tacctatact tcgagtgcta attaccatga
     2281 ttaaagctaa agatattatc actataacaa taactttcct tgctaaagat actttcttta
     2341 tagcgttact gacctccctt acagttttta ttaaccataa aatcactctt actataatac
     2401 ttgtcttaaa tatacacatt ttattttcta aataactcaa ttgcacattt ataaaaaata
     2461 tattatttga tactatcagt aaagataaag aaactaatac aattacatca atatgataag
     2521 atacagcaag agcatacata gttaaaattg caattgaaat aaatactagt attacattaa
     2581 attccatagg tatcttactg tatattagta aaaatacatt atttcttttt aaaacttcac
     2641 cttttattct gctatataaa atgatagata ataaacacac aattgctaaa cttaaacaag
     2701 ctacaaaaaa taatcttact ttttctgtag cttgtttaaa tatttgaaaa ttagtgtata
     2761 ctattccatc ttctatagtt ggctcttctg gaaaagagac atatgcctca aaggcttcat
     2821 ctactccatt tagttccata ctcagattat tcagttcaaa tttttctcca tttattgttt
     2881 taatatacga gctctttcct acattattta ttatctctat attacaatag ctcttagttt
     2941 tttgtttaaa atcatctaaa ttaccatatg atgtatttgt ataaaatact cctgtatatg
     3001 tattaataac atacactttt atatttttta gataacttaa atattcttca gaagcttgtt
     3061 tattaatcat gattttatca tactcagaat cattactact taaatctata cttttacttt
     3121 ttgtttggag tttacttttt tcttcactag ttaactgact atccacagat atcttaaaat
     3181 tagataataa gctttccact gttccttcat caaatatatt actatcataa aaatcattag
     3241 atatattaaa aatcccactc tttgtagaag ttttttgtat attgtattta ggctcagcaa
     3301 gtcctattgt agccaacaat atagttaatg gaaatgagat agatattatg attaatgaaa
     3361 ctaattttaa tgctctatat ttttccaagt tgcccacctc atctacattg tcagtatttt
     3421 atataaaata aagtttaaga aaagcataat ccccatagag taaattaaac tctcagagaa
     3481 aaaattaaaa catattaaaa ttcctaacat ttgtgtaaat gtaaaaatga ctaaaatcaa
     3541 aatgcaatta aatattaaac tcttttcttt tataattttt ccactaaaat ttttcaatct
     3601 tataaccaac tccccacacc acctttaaaa atcttggttc ttttggattt atttcaatct
     3661 tttctcttat tcgtctgata tgtactgcta ctgtattttc tgaattaaaa ctttcctcat
     3721 tccatacttt ttcgtatatc tcatctattg agaaaactcg tcctttattt tctaaaagaa
     3781 gttcaattat tttatactca attggggtca tttttatgtt ttcaccatca attttaactt
     3841 cttttgttag tgtatttaac tccaaagctc cactttttaa aatttcttta catgtatctt
     3901 cataattacc taaagttaca tatcgtctta agtttgattt aaccctagct ataagctcta
     3961 atagattaaa tggctttgtt atataatcat cagctccaat atttaatcct attattttat
     4021 ctgtatcttc actttttgct gaaattaaaa ttattggaat atttctttct tctcgtattt
     4081 ttacagttgc tttgattcca tctaatttag gcatcattat atccattagt attagatgaa
     4141 tgtcattctc tgttattatt tccaaagctt tcattccatc atatgcttta tatattttaa
     4201 atccttctga tttaagatat atttctattg aatcaacaat ttctttatca tcgtctacta
     4261 ctaatatatt gtacatattt tcacttcctc tattgtataa taatacttat ctatattata
     4321 caatatcttt cacttacttt ctcaacaatg actaaattgt tatattactt tggcttacat
     4381 ttttgtaact ttcccttata actatataat aaaatataaa tcttaagaaa atattatcct
     4441 aattcttaaa tatttcttaa gatttaagat aaattgagat ttatttgaat aaattgattt
     4501 gaattatgtt attttcagat tacttttatt taagtatttt gattggattc agctgaatta
     4561 atgtatatta attcttattg aacagttttt atttttgggg tggaagaatg gtggaagaat
     4621 tacatttcac taattttctt ccaccattct tccacccaaa tatacttaga aacatttatt
     4681 tcactgcaat aatagctact tatttaaatt ctatttttat acattctatc tactatatac
     4741 tgtacatatt ctat
//
LOCUS       DAMJVX010000230         4687 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMJVX010000230 DAMJVX010000000
VERSION     DAMJVX010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.236"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(124..927)
                     /gene="proC"
                     /locus_tag="PC407_003390"
     CDS             complement(124..927)
                     /gene="proC"
                     /locus_tag="PC407_003390"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2498798.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGINTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            complement(1252..3621)
                     /locus_tag="PC407_003391"
     CDS             complement(1252..3621)
                     /locus_tag="PC407_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDF2498799.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRLTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPKDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            complement(3626..4534)
                     /locus_tag="PC407_003392"
     CDS             complement(3626..4534)
                     /locus_tag="PC407_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2498800.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGVNIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
BASE COUNT         1377 a          904 c          512 g         1894 t
ORIGIN      
        1 attaaattcc tttttatagt aaattactct tattttactt ataccaaata catttatgta
       61 tagatataaa taagtactaa aaaaacaatt caagaaacca tatagtaaac ttaattacat
      121 ctgttatttg ctcatatctt tagacttatc tatacaagca acaatagatt ttataactgc
      181 tgctctaaag ccttcttctt ctaatacctt aacagcttct atagtagttc ctgctggaga
      241 acaaaccata tcttttaatt cacctggatg ttttccagtt tcaagaacca tcttagctga
      301 ccccattact gcttgagatg caaatttata agcttgttgt cttggcattc cagctaatac
      361 tgctgcatct gccatagctt ctataaacat aaatacatat gcaggagaag aaccactaat
      421 acctgtaact gtatccatta aatattctga tactatttct gcctcaccaa aagattcaaa
      481 cagagcctta atcatattta actcttcatc tgttacatta ctatttttac aaagagctga
      541 cattccctcg ccaacaaggg ctggagtatt aggcattact cttactattt ttttatctga
      601 acctataaaa ctttccagac tttctatagt tttacctgct gctatagata ctattatttt
      661 atttgcatct attaattctt taagtggagt taaagccttt tcgcatatat taggtttaag
      721 tgctactatt accacatcag aatttgtaac agctaccttt gaatcagttg tagtatttat
      781 tccaaattct tcttttactt tatctaaggc aacttggctt aaatctgata cagttatatc
      841 ttctgccttt actaatcctg actgtactat gccacctatc attgcacttc ccatattccc
      901 tgaaccaata aatcctaaag ttttcatatc aaatctcctt ttttctatta gttttatttt
      961 aaagtagcta aattgacttt attttaactg cttaaaatta tcttattttt ataaaatata
     1021 accttattat aactataata aattattctt ttgtatgtat tacttactaa tatgtgtatg
     1081 atttactaaa tataaattta acgatttttt cattaaactt aacgagctaa cttcctattt
     1141 agaaagttag ctcataatta atctaaaatt ttaaaaatgg aatcaaacca tttatacttt
     1201 attatatcag ttacgatttt atatagtaag aagttttatt tacaagcaaa cctagaatgt
     1261 ttgctcagtt cttcctatta tctcatcttg tagtgttcta cttaaattat tgaaatggtc
     1321 actatatcct gcaactctaa ctattaagtc tttataatcc ttaggattct tttgagcctc
     1381 taacagtaca tttttatcaa atacattaaa ttgtatatga tgaccatcca tattaaagta
     1441 tgctcttact agattagcca tattatctaa ccctttttct ccttgaacta ctgatggtgc
     1501 aaatctttga tttaataatg ttccaccagt ttttatgtgg tccatcttag cacaagattt
     1561 tataactgct gtaggtccat tagtatctcc acctttttca ggtgaaattc cctctgaaac
     1621 tggttttgca caaagtctac catttggact tgctcccata atttctccaa agtatatatg
     1681 acaagttgtt ggtaacatat ctactctgta ttctccacca caaacagtta gtctaccagt
     1741 tacctcattg taatatgcat tgaatatctc ttgcattatt tcatcagcat aatcatcatc
     1801 atttccatat ttaggagtct tatttgaaac taaattaagt atagcttcat atccttcaaa
     1861 attagcatca agtgctttaa gcaatgtatc catatcgaat tttttcttat caaatacatt
     1921 atattttatt gctgttaaac tatctgttat agttcctata ccaacacctt gtatatatct
     1981 agtgttgtat cttgctccac ctctttggta atccttgcct ttttctatac aatcatcaac
     2041 tattgtagac ataagtggac aaggcatgtg ttttgcacat atattttcta tcacagcatt
     2101 tccttctatt tttatgtcaa tcatataatg taattgcttt ttaaatgctt caaatagttc
     2161 ctcataagat tggaaatttc tagggtctcc agtttcaata cctatttgtt ttttactaat
     2221 tgggtcatat ccattattta atgttaattc aagtattttt ggaatgttca tatatccagt
     2281 tagaacatat gcttctttac caaaacatcc agtttcaaca caaccactgg ttccaccaag
     2341 tcttgcatct tctatagttt ttccagcttc cataagctct tgaactattg cttcagtatt
     2401 ataaaatgct ggttgacccc atccttttct tgatatttca caagctctct ttaagaattt
     2461 ttgaggagtc tttttactta tttgaacatt tgaactaggt tgtatcaatt tcatttcatc
     2521 cataacatca agtattatat aactaacttc attaacacca tcttgaccat ctggatttat
     2581 tccaccagtg tttatatttg caaaatctgt atatgtgcta ctttctttta atgttatacc
     2641 aacttttggt ggtgctggct gattgttaaa tttaacccat aaacattcaa gtaattcttg
     2701 tgctctatct ctatctagta ttccattttc tacatctctt tcatagaaag gatttagatg
     2761 ttggtcaagt cttcctggag taaaagcatc ccatatatta agttcagtag taacccctat
     2821 atgaacaaac caatacattt gtatagcttg atgataagtt tctggtttgt gagctggtac
     2881 aacatcacaa gtttcagcaa ttagtaataa atcttttttc ttagcttcat ctgtttcttc
     2941 ttctgccatt tgtcttgctt tctcagcata tcttttacct aatataacca ttgcatcaca
     3001 agctattgcc atagcttcta aatctgcttt tttattatat gcatctaaat cattaagaaa
     3061 atctagctct tttaatcttg cttctatgtc tttctttaaa tctaaaaatc cttttttata
     3121 tattgtatct ccacaaactg tatgtcctgg ggctctttgt tccataaact ctgtaaacat
     3181 acctgcttca tatgctgcta accactcagg tgtcattgca tttattatct tatctcttgt
     3241 ttgtcttttt ttccaaaaag gaattatttc ttctttatgt atttttctag cttcttctga
     3301 tacgctaaaa tttattatat ctctattgtg cattacttct aaatcttcca ttgtgtgaca
     3361 acaaatttct ggatatgttg gtgctccatt aggagaatca cctttttcac ctactattaa
     3421 ttctccatca tttatgctaa gagttctatt ctcaatataa tgcttaaatg ataaagcacg
     3481 aagtacagga atctctacac ttccctcata ttttttataa gcttctgtca taagtactgc
     3541 tctttcaata gatatgtgtg gttctgcatt aatactttct tctcttaatt ttttagttct
     3601 ctcaaaagtt cctcttgcca ttttattacc ctccaatctt agtattgaaa ttacttgctt
     3661 caaacagtct ttttatttcc tctaattttt cttctgatgg tctttgtagt tcttctcctt
     3721 catacttttt ataaatttta tcatacttgt gtttaccaat attatgatat ggaagaagac
     3781 ttactgcttg tatattcaaa ggctttaaaa actctatcat ttttttaacc tctaaatttt
     3841 catcatctac atttactcct acaattaatg gtattcttat atttatgtta acccctaatt
     3901 cacttaaaat cttaacattt ttcagtatta agtcatttga ttttcctgta aattttatgt
     3961 gcttatcctc atcaataagc ttaatatcat ataaaaacaa atctgcacat ttagcaactc
     4021 tttcataatt ttctgacttt gcatagccac atgtatcaat tgctacatgg attccctttg
     4081 atttgcacat gtttattact ccacaaatga agtccatatc ttgagccatt acttctccac
     4141 ctgatagtgt tactccacca cccgattctt catagaaaat tctatccttc tctatctctt
     4201 taaccaagtc tcttacagaa tactcttgac caacaatctc tcttgcattg tttacacaat
     4261 agtctaagca agtttcacaa aactcacact tatcttggtc taagcatatt tttgtttcac
     4321 ctttgtaaat tgccttatga gggcatatat ttatacatgc ttcacatttt gaacatcttt
     4381 cttcattata caaaacttgt tttgtatatg tttgactctc tggattatga caccaaacac
     4441 attcaagtgg acacccttta aaaaatactg tacttcttat ccctggtcca tcatgtatgc
     4501 tacatttttg taagtttata actaatggat tcatgatttt tcacccctaa ataatatcca
     4561 ttccattttt tcatttttat gcaattcatc ttcatagaat atacctatga aaagcatttc
     4621 tacaattaca taatactata tttgttatta ttttgtctat atatttttag taatattaaa
     4681 aaatttt
//
LOCUS       DAMJVX010000231         4652 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMJVX010000231 DAMJVX010000000
VERSION     DAMJVX010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.237"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            17..415
                     /locus_tag="PC407_003393"
     CDS             17..415
                     /locus_tag="PC407_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2498801.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSEKYKLKARNVK
                     INLNIKGYKFLPYVKNLKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            403..1341
                     /locus_tag="PC407_003394"
     CDS             403..1341
                     /locus_tag="PC407_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2498802.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIRH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKTIILGVCGKLILVPSIFLPISIFLGFRNVELTSLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1725..1949)
                     /gene="secG"
                     /locus_tag="PC407_003395"
     CDS             complement(1725..1949)
                     /gene="secG"
                     /locus_tag="PC407_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDF2498803.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2067..2573)
                     /locus_tag="PC407_003396"
     CDS             complement(2067..2573)
                     /locus_tag="PC407_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2498804.1"
                     /translation="MNLEIIRATSENAIEMGFIHSCSWRKAYKGIIPDKIIDEFTSEK
                     RAEVFQSVIPKAEEEYYLFKVNGIPAGFASLNRSQEDNAPKCVGEIYSIYFHPDFWGT
                     SVTKKGLQFCIDRLSNLGYSYITIWVLKDNTRAINFYKKNGFTCDDFEKEIHIGKKLL
                     EVRYSKKI"
     gene            2658..3086
                     /locus_tag="PC407_003397"
     CDS             2658..3086
                     /locus_tag="PC407_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2498805.1"
                     /translation="MNPVKELNNLWHIVSMQMKTEMCSGKYPTIHGISLVELSILEIV
                     EQYPNSMMKEISEILELPKSTLTSAVKRLELKKFIKKNPCIKDKRRYNLVLTELGLKA
                     QQEHRDIEIIVFRNLLHQLDKDEIETFIRIFSKAVNKSKK"
     gene            complement(3206..4498)
                     /gene="eno"
                     /locus_tag="PC407_003398"
     CDS             complement(3206..4498)
                     /gene="eno"
                     /locus_tag="PC407_003398"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDF2498806.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTERLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1582 a          750 c          572 g         1748 t
ORIGIN      
        1 atcttaagga gttgccatga taaaaaaaat agagcatgtg gtcattacta ataataatat
       61 agaaaattat atcaattttt ataaaaaatt tggtttttca tctaaagaaa tgagcgaaaa
      121 gtataaactt aaggctagaa atgttaagat aaatcttaac ataaaaggtt ataaattttt
      181 accttatgtt aaaaatttaa aagcaagtag agataactta tgttttaaaa taaacacaaa
      241 tattgatgag ccaaaaaaat acttagaaca aaatgagatt caaattaagc gaggaattgt
      301 agaatgtaca ggtgtctttg gtaatatgaa atctatttat ttgaaaaaaa ctaatggaaa
      361 tttaattgaa ttatcaaatc atagaatcac ggagagtaaa ccttggaaaa tttaatccta
      421 tcaataaatg ttgttcttcc tttgtttctc acaatgtcac ttggatatgt tctaaaaaaa
      481 ttaggtctat ttgatactca tactttaaat aaaatgaata atgtctgctt caagtctttc
      541 ttgccattat tattattttt taatatatac aatacagatt tagatggttc tcttaatttt
      601 aagcttatgg ctgtagctgt tataattatt ttttcaatct tcttaatctt agtctttatt
      661 attcctaaaa ttgaaaaaga taataagaca aggggtgcat taatacaagg tgtatttcgt
      721 agcaatttcg ttatatttgg tattcctgtg acaatttcac tatttggcga taagagtatt
      781 ggagtcacat ctttattaat tgcagttatt gtacctatgt ttaatgtatt atctgttgta
      841 attcttgaaa tttttagagg tggcaaagta aatataagac atatgattaa aagcgtctta
      901 acaaatccac tcataatcgc ctcttttata ggattgacaa tgctaatttt aaatattaat
      961 atacctacat ttttacacaa aactattggt gatatttcta aaatagctac tcctttatca
     1021 cttataattc taggcggatc ttttagtttt tctgctataa aggggcatat aaaaaccatt
     1081 atattgggcg tatgtggaaa acttatactt gtaccttcta tatttttacc aattagcatt
     1141 tttcttggat ttagaaatgt agaattaact tcacttatga taatgttatc tgctccaact
     1201 gcagtttctt catttacaat ggcacaacag atggattctg acagtgaatt agctggacaa
     1261 attgtagtat ttacttctgg attttctata attacagtat ttttaataat atttatttta
     1321 aagcagaata atcttattta atgatatttg ataacacaaa ttggtattat gagaaacata
     1381 tataaaattg atttaattgc gtaaaaacat attattatac tatttttact atataatata
     1441 actttgtgta tattatctca ttaaacatgg tgaaagtagt gttgctatat attttttagt
     1501 ttgaaaaaaa tatataaata taaacaatat ataaatacaa ataataaatg ccaacaaata
     1561 gtcaatctat aaaataaaaa ctggactaat catataaaat cattagtcca gcttttattt
     1621 tacgtacttt ttatgttgat gtacttttat taaaaactaa atttaatttt taaatatcta
     1681 gcaaatgcac taattaaata atggccagtt attcaacaat atcactactt cttaactata
     1741 agtaatgcca atgcatttat aaaaaataat actgctgtta tctttgtaac tctagcaaga
     1801 aatgcttgtt ttccttttgg tttaaagtaa gcttgacttc cttctccacc aaattctgaa
     1861 ggtaaagcat tttttgaatc ttgaggcatt atgctaatta ctagtaaaat ccctagcact
     1921 acttgcacac ccattaaaat gttgctcatt attgcccctc ctatctattt attacttaaa
     1981 tttcattact ctttagtgta tcacaataat atagtcattt caagactatt gaagtataaa
     2041 taacaaacaa aaataatatg caattactat atcttttttg aataacgtac ttctaataat
     2101 ttttttccaa tatgtatctc tttctcaaaa tcatcacaag taaaaccatt ttttttatag
     2161 aaatttatag ctctagtatt gtcttttaga acccatatcg ttatatatga atatcctaaa
     2221 ttgcttaatc tatcaatgca aaattgtaat cctttttttg tcacagatgt tccccaaaaa
     2281 tcgggatgga aatatataga atatatttct ccaacacatt ttggagcatt atcctcttga
     2341 cttctattta atgaagcaaa accagcaggt ataccattca ctttaaaaag ataatattcc
     2401 tcttcagctt ttggaataac actttgaaaa acttctgctc ttttctcaga agtgaattca
     2461 tctattatct tatctggaat aattccttta tacgcttttc tccacgaaca agaatgtata
     2521 aaacccattt ctatagcatt ttcacttgta gctcgtataa tctctaaatt cataaaaaca
     2581 ccatcctatt gacaaattaa tacgattatc gtactatatt agtgcgataa ttgtatcata
     2641 tatggaggta aaagtcaatg aatcctgtaa aagagctaaa caacctgtgg catattgtta
     2701 gtatgcagat gaaaacagaa atgtgtagtg gtaagtatcc tactattcat ggtatttctt
     2761 tagtagaatt aagtatttta gaaatagttg aacagtatcc caatagtatg atgaaagaaa
     2821 ttagtgaaat tttagaattg ccaaaaagca cattaacaag tgctgtaaaa cgtcttgaat
     2881 taaaaaaatt tattaaaaag aatccctgta ttaaagacaa aaggaggtat aacttagtct
     2941 taactgaatt aggtttaaag gctcaacaag aacatagaga catagaaata attgtattta
     3001 gaaatctctt acatcaatta gataaagatg aaatagaaac ttttattcgt attttttcta
     3061 aagctgtgaa taaaagtaag aaatgaaata taaaaaaagg ataagtgaat tgaaaaatca
     3121 actatcactt atcccttata agataaattt ttatctttta tgttatcttt ttaatcttta
     3181 atgccatatt tttaatcttc aataactatt tttttaaatt atagaaagat tttaatccac
     3241 agtatctagc ttgttctcca accatttctt ctattcttaa taattggttg tacttagcaa
     3301 ctctatcagt tcttgatgga gcaccagttt ttatttgacc agcatttact gcaacagcta
     3361 aatctgctat agtagaatct tcagtttctc ctgatctgtg agatataaca gcagtatatc
     3421 ctgctctctt agccatttct atagcatcta aagtttcagt tattgtacct atttgattaa
     3481 ctttaactaa tatagagtta gctactccat tttcaattcc tttttctaat ctctctgtgt
     3541 tagtaacaaa taagtcgtca ccaactaatt gtaatttatt tcctaatctt tcagttaata
     3601 acttccatcc gtcccaatct tcttcgtcta aaccatcttc tatagttatt attgggaagt
     3661 tgtttgacca atcttcatat aaagcaacca tttcagcagc agttaactct tttccttctc
     3721 ctgctaaaac gtatttttta gtttctttgt tgtacatttc tgtagcagca acgtcaagtc
     3781 ctaacataac atcttcacct ggcttatatc cagcttttgt tatagcttca actattaatt
     3841 ctaaagcttc tctgtttgaa cctaagtttg gagcaaatcc accttcgtca ccaacaccac
     3901 aagctaatcc tttttctcct aaaactttct ttaatgaatg gaatacttct gctcccattc
     3961 ttaaaccttc tttaaagcaa caagctccta ctggtaatat catgaactct tgaacatcaa
     4021 cgttattatc agcatgctct ccaccgttta atatgttcat cattggaaca ggtaattgtt
     4081 tagcatttac tccacctaaa tattggaata atggtaaacc tatttcatca gcagctgctc
     4141 tagctactgc cattgaaaca cctaatatag cattagctcc taatttacct ttgtttggag
     4201 ttccatctaa ttctatcatg attgcatcta tagctggttg gtcaaatgca tccattcctt
     4261 ctatttcagg tgctattatt tcatttacgt tagcaacagc tgtttctaca ccttttccta
     4321 agtatcttcc cttatctcca tctcttaatt caacagcttc aaaagctcct gtagatgctc
     4381 ctgatggaac tattgctctt cccattgctc catcttctaa tactacttca acttctacag
     4441 ttggatttcc tcttgagtct aatacttctc tagcatatac taattcaata actgacattt
     4501 gtatatctcc cttcgatttt ctattagtta aattttcatt tactgtaaac atccgtctac
     4561 tattttaatc tactttctat attatagtac ataaagttgc ttttgaaaat taggtacaaa
     4621 tttatttgat gggtaaattt gtaaaagttc ct
//
LOCUS       DAMJVX010000232         4533 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.238, whole genome shotgun sequence.
ACCESSION   DAMJVX010000232 DAMJVX010000000
VERSION     DAMJVX010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.238"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            202..1008
                     /locus_tag="PC407_003399"
     CDS             202..1008
                     /locus_tag="PC407_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2498807.1"
                     /translation="MGMILSTLGINLYLAPAKLLSGGVAGICVILYKLFGINQGISSF
                     LMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLTQDIAKYFPIDDTMLQCIYGGAL
                     TGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNTFLFINFFVVCAGAFLFGAKLVM
                     YTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEISKVIMNKMGRGVTFLEAEGAYT
                     QNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNNVEEVKGGGFKDKFL"
     gene            1135..1407
                     /locus_tag="PC407_003400"
     CDS             1135..1407
                     /locus_tag="PC407_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498808.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(1570..2142)
                     /locus_tag="PC407_003401"
     CDS             complement(1570..2142)
                     /locus_tag="PC407_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2498809.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            2326..3168
                     /locus_tag="PC407_003402"
     CDS             2326..3168
                     /locus_tag="PC407_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2498810.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            complement(3271..4347)
                     /locus_tag="PC407_003403"
     CDS             complement(3271..4347)
                     /locus_tag="PC407_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2498811.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
BASE COUNT         1651 a          586 c          709 g         1587 t
ORIGIN      
        1 ataaatatat taaaagaagg attaagacta ataacaattc aaaataaata acttatgtaa
       61 gaaccgtagg aactacgggg atagcttgtg gagaattagt aagacatatt ttagtatgca
      121 aaattctatg aagcaggaac cctgtgactt tagtcatggg aggttcagaa taacatattt
      181 ttacaatatg gtctgattat attgggtatg attctatcaa ccttaggaat aaatttatat
      241 ctagcaccag ccaagttatt aagtggagga gtagctggaa tttgtgttat attatacaaa
      301 ttatttggta taaatcaggg aatatcaagt ttcttgatga acataccaat atttattata
      361 gctagaaagt attttgataa taaatttttg ctaataagtt ttgtcaatat gttactgttt
      421 tctctagcac tggggctaac acaagatatt gctaaatatt tcccaataga tgatactatg
      481 ctacagtgta tttatggtgg tgcattaact ggtataggta tggggttaac ttttaaagca
      541 agagctactg ctggaggact tgatataata gcagccataa tgaaaagaaa gtatgatata
      601 cctatgaaaa atacattttt atttattaat ttttttgtag tatgtgcagg agctttctta
      661 tttggagcta aattggtgat gtacacttta attacaatgt atataatttc ttttactatg
      721 gatataggaa aagattgctt tgatagaaaa aaatctatac ttttaatatc aaataaatat
      781 gatgaaatat ccaaagtgat aatgaataaa atgggtagag gagtcacttt tttggaggca
      841 gaaggtgcat atacacaaaa taagaaaaag atgatttatt gcataatatc tgcaaatgaa
      901 ataggtaaat ttaaagattt aatttatgat atggatagtg aagcttttat ttctgtcaat
      961 aacgttgaag aagtaaaagg tggaggtttt aaggataaat ttctttaaga ttatatatgt
     1021 taattatata tgttaaaaaa tgccatatgt attgagatta tacctagtta gatgtatact
     1081 gtagttaatg gggtacatga agtattcaat aatatactag gaggtataag aattatgtta
     1141 agagagagat ttgtagtaag aaatcatcat aatccacata caagtgggtg ttgtgggact
     1201 ggaggagaag gatgctgtgg aggagataac catgaacatt cacatcaaca ttctcacagt
     1261 catgggcatg aacatgagca tgtaaatggt gtaggtcacg accataatca tgatgatggt
     1321 ttttcaggta ctcatgagca tagccatgag catacacata cacatggtca tgaacattca
     1381 gatgggcata cacatgaaca taaataaaaa cattttaaat ttataagaga tttttatttt
     1441 gtgattccat gaaggtttaa ataaaaattg aatgttttaa gtaaaaatta ttgagaataa
     1501 ataagaaagt gaactgttaa agtagataac ttaaatagtt cacttttatt aattatctaa
     1561 gtattaagtt taaatccaat gattaactaa aaaagaagct atattttcat aaggtatttt
     1621 ttctgttttt aataaacttt ggacaactaa accatcaact attgacatga gtaaaaaaga
     1681 aaatgattca gcatcctgtt cattttcgct actattatat ttttctagtg attcaacaat
     1741 ttcagattta agagtagttc tccattcaat atattttaat cttattcttt cctttaaatc
     1801 attatttgaa gtaattgcct cacaaataag atacatatgt accctaccac gactttctat
     1861 agtagatatt ttctctaaga ttaagttaac catttctatt tgagaatttt ttgatttgac
     1921 attttttaca cagtttagta tagcactagt tataacttct aaatgagtat ccgcaatatc
     1981 atatataata tcatctttag atgcgtagtg ataatacaaa gtacctttac ttatatttac
     2041 atcttttgca atatcagcaa ggctagtatt ttgaactcca tttttggcta ttaaattggt
     2101 tgcagatgat aaaatagcct gtttcatatt atttgttttc ataaaaccta cctttccatg
     2161 tatatttata acaaattaat aacattataa gcttatgaat atttattgtc aataaagcga
     2221 gaatctatat ctattgactt tttttcattc aatatataaa ataataatta aatcggtcgt
     2281 acgaccgact gaataaataa aataatatct tatagtgagg ttaatatgga taaaataaaa
     2341 ttgatagtgg acagtgcatg tgacttacct gatgatataa tagaaaaata caacatagaa
     2401 gttgttggtt taaatgtatc ttttggagaa gaaagttata tatctggaaa ggaaatagat
     2461 aatgaaactt tctatagaaa aatgagtgag agtaaagtac taccaaaaac atcatgtcct
     2521 tctcctgata aatttttaga agcataccat tgtcaggaag aaagtgtatt ggtgattaat
     2581 ataagttcag gattatctgg gacttataat agtgcacttt tagcaaaaga tatgtttgaa
     2641 aaggaaggaa ataacaaaaa agttgaggtt atagattcac ttagtgggtc aattggaata
     2701 ggacaattag tgttaaaagc agcaatatta ataaaagaag gaaaagaaat tgaagagatt
     2761 gtttcaataa taaataaata taaagataat cctccatttt ttggaacact tgaaacttta
     2821 gaaaatgcta ttaagggtgg tagaataaat ccaatagcag gaaaaattat aaataccttg
     2881 aattttaagg cgataataca agttgcagat ggcgttgtaa ctcctataga taaggcaaga
     2941 ggtgaaggaa acagtttgaa aaagctgatt actttagtag agtcaaaaat taaagataaa
     3001 gaagaaaagg ttttatttat aggacatgct aattgcccag aaaaggctca aaaggtaagg
     3061 gaagttatgg aaaaggatgt aaaatacaaa gatgttgtaa tctgtgaaat tggttcagtg
     3121 atggggacat atacttcaaa gggtgcaatc ttaataactg caatataaat aatggattga
     3181 aataaatcat agattgagaa attaaagcca tataaaaatg tttaaaacat ttttatatgg
     3241 ctttttaatt ttatcaaatt gattttagaa ttaaccaact acacatattt ctttgtcatg
     3301 tttgtttaat atctcacaac catcttcagt aacaagtact aaatcttcta ttctaactcc
     3361 gaactcacct ggtaaatata tacctggctc tatagagaat atcattcctg gttgtacagt
     3421 atctgtattt atagaagata catctccttt atcatgaact tcaagtccta tagagtgacc
     3481 agttctatga gtgaagtact gtccatatcc cttttcagtt atgtagtcac gagcagcagc
     3541 atctatatca cagaatctaa cacctggttt tacaatagct tcagctctct tattagcttc
     3601 aagaactatt tcaaatattt ctctaccttt ttcaggtatt tctttataga atacagttct
     3661 tgtcatatca gcacaatagt tatcttttat acaacctata tctaatatta ttgcatctcc
     3721 tggtttcaca agtgcatttc caggttcacc atgtggatta gctccattag gtccaaaacc
     3781 tataataggg tcaaatgaaa atccatcagt acctaggtct tcatatattt tagataattt
     3841 accaacaagt tgtttctctg taagttcacc agaaacagta gctttaagtt gttccatagc
     3901 cttgtcattt aattttgaag ctattctcat taactctttt tcttcttcat ctttacacat
     3961 tcttaaagta tctattatat aagatgaatt tacaaactta cttcctccac ctaattctat
     4021 taagtttaat aagaatctag ctggccagtt tttatcaaca cccatagtag catttttgtc
     4081 tatatgttca gttattattt ctactggatt ttgagtatca ttaaaccaaa ccatttcaac
     4141 acctaaatct tctgaaactg ggaataattc atttataaat aatttattat tgccatttaa
     4201 atttaagtat aatgctaata atctttcacc tggatggatt agtactcctg taagataaaa
     4261 tatagaagta gggtcactta ctaacatttg agaaatatca tcttttttca tttgttcaag
     4321 tacagcattt aatctttgag ctttcataat aatttcctcc taatcaaaat tcaatacaaa
     4381 tcaattttag cattattaaa caaaaaagag tatacttttc ttctagaaat ataaaaaaag
     4441 tttgttatat attttttata ttaagtttaa tctctacttt tgcattaaat acggaagtaa
     4501 aatgactttt tattgtgaaa attctataaa agt
//
LOCUS       DAMJVX010000233         4460 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMJVX010000233 DAMJVX010000000
VERSION     DAMJVX010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.240"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            152..1627
                     /locus_tag="PC407_003404"
     CDS             152..1627
                     /locus_tag="PC407_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2498812.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1714..2127
                     /gene="speD"
                     /locus_tag="PC407_003405"
     CDS             1714..2127
                     /gene="speD"
                     /locus_tag="PC407_003405"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDF2498813.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2157..3008
                     /gene="speE"
                     /locus_tag="PC407_003406"
     CDS             2157..3008
                     /gene="speE"
                     /locus_tag="PC407_003406"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDF2498814.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            2998..3876
                     /gene="speB"
                     /locus_tag="PC407_003407"
     CDS             2998..3876
                     /gene="speB"
                     /locus_tag="PC407_003407"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDF2498815.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4056..4256)
                     /locus_tag="PC407_003408"
     CDS             complement(4056..4256)
                     /locus_tag="PC407_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2498816.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1764 a          499 c          764 g         1433 t
ORIGIN      
        1 tttgagtaac ttcaaaaaaa cacttgtaaa aatgctggaa tgttaatata ataaatatca
       61 tgagtttaat aaaagtaaaa ataaaaaata gaaataataa ataataaata ttaaaaatat
      121 ttgaatatag agaggagatg aatttgaaat catggatagc gagtttgcaa atgcagttga
      181 attaagctat gagtgtgctc cattattaga gtcgttaaag gaatactcag agaaagatat
      241 agcttgtttt gatgttcctg gacatgtaaa aaatagaggt gtagctatat taaataaata
      301 tctaggtgaa agtctcatga aaatggatat aaattcatca ccaactatgg ataatgtatc
      361 tgcgccaaat gggattataa aaaatgcaca agatttgcta gcacaagcat atatggctga
      421 tgaggcattc tttataacga atggcacaac tcaagcaata catgcaatga ttttgagtgt
      481 tataaaacca ggtgaaaaag tattacttcc aagaaatata cataaatcag taataaatgc
      541 gttaatactt tgtggaggaa taccaatatt tatacaacca gaatttgatg aaaaactagg
      601 tataagttta aatattactc ttgaaaaagt tagaacagag atagaaaaag attgtaatat
      661 aaaagcatta tttttcctta atccaactta ctatggagtt tgtgcagacc ttgagagtat
      721 tatagaacta tgccataaaa ataatgtttt agtgttagtt gatgaagctc atggtgcaca
      781 ttttccattt catttagatt tacctccttc tgcaataagt ttaggagctg acatggtagc
      841 tgtaagtata cataaaactg gaggagcatt gacacaatct tcagcactct tattgaatag
      901 agataatgta agttttgaaa aggttcttca atcaatcaat atgctacaat caacatcagc
      961 atcttatttg cttatggcaa gtatagatgg tgctagagtt aatctagttg agaatggtga
     1021 aaagcaactg tcaaaagcat taaatctatc taggtatgca aaatctaaat tgaacaaaat
     1081 tgatggaata aaagttttat ctacagaaat attgaaacaa aaaggtgtta aatttataga
     1141 tgaaacaaaa ttgtgtataa atgtaaaaga attaaatttg actggatttg aagtatacga
     1201 tttattatat aaaaattttt caattcaagt agaattggga gattcatata atatactggc
     1261 acttgtgtcc attggaacaa ataaatcaga tatagataga ttagtaaaag cactttctat
     1321 aattgcaaag atttatagaa aagaatcaac attaaatgaa tttaatatgg ttcaaataaa
     1381 tccaattatt aaacttaatc caagagaagc tttttatgca ccaaaggaaa gtgtagaaat
     1441 caattcatgt atagatagaa tttgtggaga atcaataatg gcatatccac caggtatacc
     1501 gattattgct ccaggagaat tgataacaga agaaattatg gagtatatta ttttcttaaa
     1561 aaatagcaat gcgtatctta cagatgtaca agataaaaac ttagatagaa tactagtaat
     1621 aaaataaatt actttatttt gcaagggcaa gtgtttgtta agtagagttt atcaaataga
     1681 acttattaaa agtataaagg agttggggtt gaaatgaaat ttgaaacact aggaagacat
     1741 atactagtag aatactataa ttgtgatgaa aacgtactta aagacccacg tcttatagaa
     1801 aattttatga atgattcagc attaaatgca aaagcgacaa ttgtggattc tgtattccac
     1861 cattttaatc cttggggggt ttcaggagct gttataattg ctgaatctca tttgacaata
     1921 catacatggc ctgaatatgg ttatgcagca gcagattttt ttacttgtgg tgatatagac
     1981 ccatggaaaa gttttgaatt attagagcaa cttttaaagt ctgaaagaag cgagtctaca
     2041 gaaattccaa gaggcttaac tacaaaaatt aaaaaatatt caaaaaaaga tttaggtaag
     2101 attactcata aaccagaagc agtataaaac atacaagaga aagtgaggat tattatatgg
     2161 aactttggta tacagaagaa tggacagaaa atgtaagatt ttctataaaa gtaaataaac
     2221 atctatttga aggaaagtct caatttcaaa ggatagatgt atttgatagt gatgaatttg
     2281 gaaaatttct aacaatagat ggattaatga tggtaacata taaagatgaa tttatatacc
     2341 atgagatgat aactcatgtt cctatggcta caaatttaaa cataaagaaa gtattagtca
     2401 taggtggagg tgatggaggt acagtaagag aattaagccg ttatcctcaa atagaaaaga
     2461 ttgacatggt tgaaatagat aaaatggttg tagatgtatc taaggagtat atggatatat
     2521 gctcatgtaa attagatgat aaaagagtga gtttatattt tgaagatggt gttaattttg
     2581 ttaagtgtgc acatgataaa tcatatgacc tgataatagt agattcaact gacccaatag
     2641 gaccaggcga agggcttttc tcaactgatt tttataagga ttgttataga atactgactg
     2701 atgatggaat tttagtaaat caaagtgaaa gtccgtattt tgactttaat gctaaagaga
     2761 tgaaaagagc taataaaaaa ttaaaacaga tatttccaat atcagaagtt tatcaagcac
     2821 atataccaac atatccatct ggtcactggt tgtttggatt tgcttcaaaa aaacttaatc
     2881 cagtaaaaaa tcaagataga aatggatggg agaaattaag tttaaaaact aaatattata
     2941 atagtgatat acatttaggt tcgtttatgt tacctcaata cgtgaaggag atgctagatg
     3001 aagaataatt tctatcatat gaacactttt atgagtatgg acaaaaatta tgaagaatct
     3061 aatcttatag tatttggtgt tggatttgat ggaactactt ctaacagacc tggggctaga
     3121 tttgcaagta gctctatgag aaaagaattt tacggtcttg agacatatag tcctttttta
     3181 gatttggact tagaggatta taatatatgt gattatggag atttagaaat tagtgttgga
     3241 agtacagaac aagtcttaaa agaaatctat caagagacat ataagattgt tagagattca
     3301 aaggtaccct ttatgattgg aggagagcat ttagttacat taccagcctt taaagcagta
     3361 catgaaaagt acaatgatat atatgtaatt cattttgatg cccatactga tttgagggaa
     3421 gaatataata atagtaaaaa ttctcatgca acagtaatta aaagaatatg ggatattgta
     3481 ggtgataata aaatatttca atttggtata aggtctggga caaaagaaga atttaaattt
     3541 gctacagaag aaaaacacac atacatggaa ataggaggaa tagatacgtt tgaaaatata
     3601 gttaatatgc taaatggaaa gaatatttat ctaactatag atttagatgt attggatgca
     3661 tctgtgtttc cgggaacagg tacaccagaa cctggtggtg taaattatag agagtttcaa
     3721 gagattttta agattataaa aaactctaat ataaatatag ttggttgtga tattgtagag
     3781 ttaagccctg attacgatac aacaggtgta tcgacagtta tagcttgtaa aatcttaaga
     3841 gagttatgct taataatatc tgataaaatt aaatagagta ttttatataa atagtatata
     3901 ttataatata agaagttatt ttaaaaaagt tagtttaagt ttgacgcttt tctaaagtaa
     3961 cttcttattt ttgtaattaa aaataaatat aataaacaac aaaaaagagc cctcgatagc
     4021 tccttttcta aaatatatat atttaaatta aataattata ttctatttac gttttcagct
     4081 tgaggtcctc tattaccttg agttatatca aagctaacct tttcgccttc ttctaaagtt
     4141 ttgtatccat cattttgtat agctgagaaa tgtacgaaaa catcatcttc tccctctaca
     4201 gatatgaacc caaatccttt ttcattgtta aaccatttta ctattccgtt tttcattata
     4261 agaaaacctc cgcattatta aaaaatacaa actaaaaaaa taagtctgtt ctattaatat
     4321 aacataaatt ttaaataaaa gcaaataaaa tttttaaaaa aattaattac ttttagaatt
     4381 taataaaaat tgttaaatta tacagtgaga agtattgtaa aatttgacga aaatgtaaat
     4441 gtagataata aatttaatgt
//
LOCUS       DAMJVX010000234         4447 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.241, whole genome shotgun sequence.
ACCESSION   DAMJVX010000234 DAMJVX010000000
VERSION     DAMJVX010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.241"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            140..787
                     /locus_tag="PC407_003409"
     CDS             140..787
                     /locus_tag="PC407_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2498817.1"
                     /translation="MNILIADDEISMLKILCAYFKKENFNVFTAKNGEEALDIFYENK
                     IDLAILDWMMPIIDGIEVCKEIKENSNTRVLMLTAKSQSEDEIEALNIGADEYIKKPF
                     DPRVLIIRAKKLINYKNTINIKDLKIDFGSSKVFKGDKELLLTKKELELIRCLINNKG
                     MVLSREKLLDLVWGLDYEGDFRTVDTHIRRLRVKIGEDIIKTYRGLGYSLEDFND"
     gene            780..2174
                     /locus_tag="PC407_003410"
     CDS             780..2174
                     /locus_tag="PC407_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2498818.1"
                     /translation="MTKLNKKLSISISIVVVVVYIISIIINSMFIHRYYLHEKRNILD
                     NVENEIKDKDIDKLKSDITLIEEKNNIDIVYIELDKNHKDKGNIDKINQDLLNAFWKK
                     GLSLNKFWIEENSLNNIDKKSINKIYNQGKTKYSLLVKFIKKDNYLFAISIPIEHSEE
                     TIGIVNRFNIIMGIFSVVIITILTFILSNRVIKPMERLKFLSKDISELNFRTEDIKTN
                     DEIEELAYSINIMSEKLEKAHNELNKRNENLKTFISDASHEMKTPIALIKAYAIGMKD
                     GLDDGTYTDTIIEQAENMTNTINSLLYWAKYEKKERSLCLVDLKERLYKSLKNYELLI
                     ERGSISVKCSVDDKELIISSDEDSIDMVFNNLISNAIKYTNNNEIEINLFEENERIIL
                     SIKNGIDYNMGDDIENMWKPFYVLEKSRSKELSGTGLGLTIVRTILEENNFRYRVEIC
                     DGIIEFYIIFIKVI"
     gene            2599..>4447
                     /locus_tag="PC407_003411"
     CDS             2599..>4447
                     /locus_tag="PC407_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2498819.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAITVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSHGGGSSSSKVDKVVLKNTITAANKLYNEAIEGTNVGEYKVGSKAIYKTAIDKAQAI
                     LDKSGVTQKEVNDAVTALNTATDTFKAGKVVAVDKTALQDAVTAATALHA"
BASE COUNT         1935 a          430 c          777 g         1305 t
ORIGIN      
        1 gttaagacat tgtcgagata ttataatata taactaatgt attataaata gttgtaaact
       61 tcctgtaaca tatattttat ataattaaag taatggatat aatatgaaat ataataatga
      121 attttaggga tgtgaataaa tgaacatatt aattgctgat gatgaaatta gtatgcttaa
      181 gatactttgt gcatatttta aaaaagaaaa cttcaatgtg tttactgcaa aaaatgggga
      241 agaggcttta gatatttttt atgaaaataa gatagactta gctatattag actggatgat
      301 gccaataatt gatggaatag aggtttgtaa agagataaag gaaaatagta atacaagggt
      361 acttatgttg actgccaaaa gtcaaagtga agatgaaata gaagcactca acattggagc
      421 agatgaatat ataaaaaaac catttgatcc tagagtttta ataataagag ctaagaaact
      481 aataaattat aaaaatacaa ttaatattaa agatttaaaa atagattttg ggtcaagtaa
      541 agtttttaaa ggtgataagg aattattatt gactaagaaa gaattagaat taattcgttg
      601 tttaatcaat aataaaggaa tggttttatc tagggaaaaa cttttagatt tagtatgggg
      661 attggattat gagggagatt ttagaacagt agatactcat ataagaagat taagggtaaa
      721 aataggagaa gatataataa aaacttatag aggattaggg tatagtctgg aggattttaa
      781 tgactaaact aaacaaaaaa ttgtccataa gtatatctat agtagttgta gttgtttata
      841 taatatctat aattataaat agtatgttta ttcatagata ttatttacat gaaaaaagaa
      901 atatattgga taatgttgaa aatgaaatta aagataaaga catagataaa ttaaaatcag
      961 acattacttt aattgaagaa aaaaataata tagatatcgt atatatagaa ttagataaaa
     1021 atcataaaga taaaggtaat atagataaaa taaatcaaga tttattaaat gcattttgga
     1081 aaaaaggttt aagtttaaat aagttttgga ttgaagaaaa tagtttaaat aatattgata
     1141 agaaaagtat aaataagata tacaatcaag gtaaaacaaa atatagtttg cttgtaaaat
     1201 ttatcaagaa ggataattat ttgtttgcta tatctatacc tatagaacat tctgaagaaa
     1261 ctattggcat tgtaaataga tttaatataa taatgggaat tttttcagta gttataataa
     1321 ctatacttac atttatatta tcaaacagag ttataaaacc tatggaaagg ttaaagtttt
     1381 tatctaaaga tatatctgaa ttaaatttta ggactgaaga tataaaaacg aacgatgaaa
     1441 tagaagagct agcttacagc ataaacataa tgagcgaaaa acttgaaaaa gcacataatg
     1501 aattgaataa aagaaatgag aatctaaaaa cttttatatc agatgcatct catgaaatga
     1561 aaactccaat agcacttata aaagcatatg caataggtat gaaagatggt ttggatgatg
     1621 ggacttatac agatacaata attgaacaag cagaaaatat gaccaataca ataaattctc
     1681 ttttatattg ggcaaaatat gagaagaagg agagaagtct gtgtctggta gatttaaagg
     1741 aaaggttata taaaagttta aaaaactatg agctgttgat agaaagaggt agtataagtg
     1801 ttaaatgcag tgtagatgat aaagaactta taataagttc ggatgaagat agtatagata
     1861 tggtatttaa taatttgatt agtaatgcta taaagtatac taataataat gaaatagaga
     1921 ttaatctttt tgaagagaat gagagaatta tattatccat aaaaaatggt atagattaca
     1981 atatgggaga tgatatagaa aatatgtgga agccttttta tgttttagag aagtcgagaa
     2041 gtaaggagct ttctggtact ggtcttggtc ttaccatagt tagaactata cttgaagaaa
     2101 ataattttag atacagggtt gaaatttgcg atggaattat agaattttat attatcttta
     2161 taaaagtaat ttgaattaaa atgtaaatat aaagaataat taaaaaaata tcgcaattat
     2221 ttgtttttca tatggataaa attggttatt taatatataa ttacaagtat aaataataaa
     2281 atttatttgt aaaaagagta gcacttttaa ttctaaaggc tacttttttt atacaaaacc
     2341 atgtttttta taacaattca ttaacataaa aatcaaactt accttaaaat ataaatttac
     2401 aaatttttca atcaataaga aaatttctct attccttaca taatgactgt aatgtaacaa
     2461 aataattaca aattaattat gaaatcatta tagttagaaa cctctttaaa attagatatt
     2521 aagtatcctt tagaattaga acgggaacaa agttaaaaaa ttaagtaata tcaaatggac
     2581 aagggggaag aaaataaaat gaaaatacca aagaagattg ctgcaatgct tacagttaca
     2641 atgatagctg gaagttcaac agcaggcata gctagtgcac aaactgtggc aacaaattta
     2701 acagggcaag aaagatatga aactgctgtt aaaataagtc aagatggatg gaaaaatgct
     2761 gatgaggtag taatcgtaaa cgactcatct atagcagatg ctctatctgc aacaccattt
     2821 gcaaaagcaa aaaatgctcc aatactttta acaagtaaag ataaattaaa tgataagacg
     2881 aaagcagaaa tacaaagatt aaaagctaaa aaagtatact taataggtgg aacttctgta
     2941 ttaagtacaa atatagaaaa agaaataaaa gatttaaaaa tatcttttga gagaatctct
     3001 ggagctgaga gatatcaaac atccttagaa ttagcaaaga aattagatgc tataagtgat
     3061 gttaagaaaa tagctgtagt taatggtgaa aaaggacttg cagatgctgt aagtgtagga
     3121 gcaccagctg cacaaaacaa tatgccagta atacttgctg attctaaaaa tggaacagca
     3181 gttgcagaca aatttataaa agatgctgga ataactcaat cttatgtagt aggtggagag
     3241 agttcaattt ctgaagctgt taaaaataaa ctaccaaata gtacaagact aggtggaaca
     3301 gatagaaatg atacaaatgc aaaagtaata aaagaatttt ataaaaagac agatttaaaa
     3361 aatgcttatg taactaaaga tggtatgaat aaacaagacc aacttataga tgcactagca
     3421 gtaggtgtat taggtgctaa aaatcaatca ccagtagttt tagttggaaa gaacttatca
     3481 gcttcacaaa aatctctagt taactctaag agttttgata agataactaa agttggtggg
     3541 aatggtaatg agacagcatt taatgaaatg aagtcattac aagaagtaaa aacagttgaa
     3601 gctaaaacaa tttctgaatt aaaatcagct atagataaag ctactgctaa tgatgttata
     3661 aactttaagc caactagtga ggttaaagaa gcatttacaa tacaaactga taaggcgata
     3721 acagtaaact taaatggtac atatacaaag actgtgacta tcaacatgcc taatggtgat
     3781 gtaaataact atgcaaaagt ggatgatgta gttatagatg atgtaaaaga tggaactttt
     3841 gttaactacg gaaaaataac taacttaaag gttaatgata aaaatggagc caagatagaa
     3901 aataattcta aaggtgagat tggtagttta acagtagcat ctggagcaag tcaagtaaaa
     3961 gtgactaatg gtggtaagat aactacagtt acaaataatt ctaaaggaac tactatagat
     4021 aataaaggta caataagtag tgttaaggga gataattctc caacaataag tggaaatagt
     4081 ccaagttcta actcttcagg aggaagttca tcaagtggag gttcttcaca tggaggagga
     4141 agttcttcat caaaagtaga taaagtagtt ttgaaaaata caataacagc agcaaataaa
     4201 ttgtataatg aagcaataga gggaacaaat gtaggtgaat ataaagtagg gtcaaaagca
     4261 atatataaaa cagcaataga caaagcacag gcaatattag acaaatctgg tgtaacacaa
     4321 aaagaagtaa atgatgcagt aacagcatta aacacagcaa cagacacatt taaagcagga
     4381 aaagtagtag cagtagataa aacagcatta caagatgcag taacagcagc aacagcatta
     4441 catgcta
//
LOCUS       DAMJVX010000235         4445 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.242, whole genome shotgun sequence.
ACCESSION   DAMJVX010000235 DAMJVX010000000
VERSION     DAMJVX010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.242"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     repeat_region   117..1071
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=316..344
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            1718..2989
                     /locus_tag="PC407_003412"
     CDS             1718..2989
                     /locus_tag="PC407_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2498820.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSIADTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPM
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
     gene            complement(3628..4383)
                     /locus_tag="PC407_003413"
     CDS             complement(3628..4383)
                     /locus_tag="PC407_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HDF2498821.1"
                     /translation="MAHICFEDICYLLLKEAMKLKLIPHNKIVLFEDDLSIGNIFDVN
                     NYELRENIFKIRNVFENNPKLLFKPDLLEDRKLSKCRNIIIWCANNPKDLCGVYYVVS
                     KFQDKNIKIALVDEKKTEDALIRYSFTSEMSPEDFPFFLDKTLEIGFNEKLEFKNKWD
                     VLVKENSIFRALVGNEIKSVEETYFDKYILRNISDKWQPLIEVVANCMFEDEMRVKDW
                     FILWRLEKMASLKIVKIKGSKGDFYSDKEIRKV"
BASE COUNT         1704 a          621 c          494 g         1626 t
ORIGIN      
        1 aaactaaagt caatgttatc tggtaattat ttactacaaa tgataaaaaa taattatatt
       61 ttaaagtaga aaatgctgta taaattattg gggtatttta cctatacttg ttgcaaattt
      121 acataccacg tagttaatat aaaacaaatt tcaagcgatt ggacaacgaa taaaaattgc
      181 aatttatata tcatgtagtt aatataaagc atatttgtgt cgatatatag ttctaactaa
      241 gtataagatt tacatacctc atagttaata taaaacaatg tgtccgaatt ggacacatag
      301 aaaggaaaag taaaaattta cataccacat agttaatata aaactatgct tatatattat
      361 gtttattctg ttgtttctaa aatttacata ccacatagtt aatataaaac gattttttca
      421 tgcggaggaa aattgatgtg gggacatatt tacataccac atagttaata taaaacagat
      481 aatctaggca cacatacaat gcgcaaaact ttatttacat accacatagt taatataaaa
      541 cattcagacg atataggcgc aggagctaag tattctgaaa tttacatacc acatagttaa
      601 tataaaacca gcacgccaag tataagtaaa acttccacta tcaccattta cataccacat
      661 agttaatata aaactaaatg ttgctccgta acctaaaaac ataatgctcc aatttacata
      721 ccacatagtt aatataaaac gtgaaagaaa aattaatatg ctatttacat tttacaaatt
      781 tacataccac atagttaata taaaaccatt ccacaattta tacaattttt tctgttctaa
      841 aacatttaca taccacatag ttaatataaa actcttctta tcttccagtt gcaaaatcta
      901 tgcttgacct atttacatac cacatagtta atataaaact agtttgagtg gtaaaagtac
      961 gaagtacccc tctttaattt acataccaca tagttaatat aaaacatatg taattcaccg
     1021 ttttaaatta tagataaatt aaatttacat accacatagt taatataaaa ccccaaaata
     1081 aactaagtat tttcagtact tacacacata caactctctc aaatttgcag tgaaccatga
     1141 gtagtgcaat tgataacatt tatcatacac cctcaatccc ttacattgca actgttaaac
     1201 cctactttat caaaaatatc gctcactgca aaatttatat atttttatta tatcataaaa
     1261 ttcaaactat aaaattaaaa tggaataatt tatcaattta agaacaccta catatttgta
     1321 aatacttact atattatcta ttctaataat tcttcttcta gctggaacta atataaaaat
     1381 catctctgaa agattaggac atactaatat aaaaataact atgaatagat attttcttgt
     1441 tttaaacgat atggataaaa aagcttttga aaatataatc aaagttctct ttaaataaca
     1501 ttccatttta tgtaagtaag cattatattt ttcttaaatg tcacttaagt gtcagttata
     1561 tataataata gtaaacaact gtatttcact aatatttgct attatctaat agtatttata
     1621 gtttagattt gatataatac atgtggtaaa attttgattt gtaaaacaaa attttaccta
     1681 catatattaa aacaaattta aaactaagga gacctatatg ttaaaagtat taaaaggaaa
     1741 tataattttt acaaaaactt cagaaacttt cacagtattt gaaaatagtt atattatact
     1801 ggaaaaaggt aaagtaaagg gaatatttaa agagatacct aaggaataca aaaacctaaa
     1861 agtagtagat tacactgata agcttataat acctggtatg aatgacctcc atgcccatgc
     1921 ctctcaattt aaaaatcttg gaatggctat ggataaagag cttttgccat ggcttgaaac
     1981 ttatacattt ccagaagagt caaattataa agatatagaa tatgcaacaa aaatgtataa
     2041 aaaatttgta aaagaacttt ggaaatctgg aactacaaga atagcagtgt ttgcaacagt
     2101 acataaagaa gcctctatga aacttatgga tatattcaaa gaagttggtc ttggagcata
     2161 cgttggtaaa gtaaatatga atatgaattg ccctgatact ttattagaaa acactcagga
     2221 ttctattgct gatacagaag aaattataaa taagtatagt gatacagatt ctattgtaaa
     2281 accaataata acacctagat ttataccaag ctgtacaagt gaacttttaa aaggtttagg
     2341 agatttatgt ttaaaatata atattcctat gcaatcacac ttatctgaaa attatgatga
     2401 gattgaatgg gttagaagtc tagaacctga atctaaattc tatggagatg catataatag
     2461 atataattta tttggtcaaa ctcctacttt aatggctcat tgtgttcatt gttctcagga
     2521 tgaaatagat ttaatgagag aaaacaatgt tatggcagtt cactgtccag cttctaactt
     2581 taatgttggt agtggtgcta tgcctatcag aaaattaatc gataataata ttagattagc
     2641 acttggatca gatataagcg gtggacatac attgtcaata tttaaagcaa tggtatctgc
     2701 aatacaatta tctaaattat attgggttaa ttctggtaag aagtataatt tcttatcttt
     2761 atctgaggct ttttatattg caaccaaaag tggtggaagt ttctttggaa aagttggtag
     2821 ctttgaagaa ggttatgatt ttgatgcatt gattattgat gattcaaatt taaatcatga
     2881 caattattct atacttgaaa gacttgaaag atttatctat gttggtgatg atagaaatat
     2941 cattcataga tatgtatgtg gaaaactaat tgaagaacct aatatataat tataataaaa
     3001 attttaatta tatattatgg ttaatttatg caactataat taataaaaga gtatatttta
     3061 aatctaaaag ctataggttg aaacatactc tttttatttt gtttatttat agctttagtt
     3121 attaatcata tataaatcta caaaacgtat tcctagtaac ttatattttt tgatatctac
     3181 aatcataaga ctatattatt ggattcatac ctcaaaaata ttgccttctg aacattatta
     3241 tattcaaagt tatgttaatt tacaatgata tatctaacat gtattgatta aaaaaaatta
     3301 aaacttgctt ttcatctcct atatctaaag aagtggcatt tagaataaac tattgtaata
     3361 ttaaagttga aattttctag tttatttact ctatagcacc atattttaat actattaagt
     3421 aaaaatgtag ttaaaatata tactaatatc cctgaaataa gtatttcttt tttatttagc
     3481 acacttattc gtaaggtatt agttattata ttttcaaatt atagaataaa tattatactt
     3541 attgctaatt ataatattaa tactgttaag atatttttct tcatagttat acccattatt
     3601 taatacaatt tattataaat tcaattttta aacttttctt atctccttat cagaataaaa
     3661 atccccttta cttcctttta tttttactat ttttaaactt gccatcttct ctaatctcca
     3721 aagtataaac caatctttta cacgcatttc atcttcaaac atacagtttg caaccacttc
     3781 aattaaaggt tgccatttgt cactgatatt tcttaatata tatttatcaa aataagtttc
     3841 ttcaacactt ttaatttcgt tacctactaa tgctctaaaa atagaatttt ctttaactaa
     3901 cacatcccat ttatttttaa attctagttt ttcattaaag ccaatttcta gtgtcttatc
     3961 taaaaagaat ggaaaatctt ctggagacat ttcactggta aatgaatatc taattagagc
     4021 atcttctgtc tttttttcat ctactaatgc aatctttata tttttatctt gaaacttact
     4081 cactacataa tatactccac acaaatcttt tgggttattt gcacaccata ttattatatt
     4141 tctacatttt gataatttcc tatcttctaa caaatctggc ttgaataata gtttcggatt
     4201 attttcaaaa acatttctaa tcttaaaaat attttctctt aattcgtagt tatttacatc
     4261 aaaaatatta cctattgata aatcatcttc aaataataca attttattat gtggtattaa
     4321 ctttaacttc attgcttctt ttaataacaa ataacaaata tcctcaaagc atatatgtgc
     4381 cataaaattc acctctttta aaattaacaa tttattttat tataatatat aaactgtaat
     4441 tatca
//
LOCUS       DAMJVX010000236         4442 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.243, whole genome shotgun sequence.
ACCESSION   DAMJVX010000236 DAMJVX010000000
VERSION     DAMJVX010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.243"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(108..653)
                     /locus_tag="PC407_003414"
     CDS             complement(108..653)
                     /locus_tag="PC407_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF2498822.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(771..2594)
                     /locus_tag="PC407_003415"
     CDS             complement(771..2594)
                     /locus_tag="PC407_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498823.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGVLASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(2607..4382)
                     /locus_tag="PC407_003416"
     CDS             complement(2607..4382)
                     /locus_tag="PC407_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2498824.1"
                     /translation="MKYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLREKIGVVFQNSSVFGKSILENIVMGNEGISREDVIKSC
                     EIANANQFITKLENSYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         1452 a          796 c          393 g         1801 t
ORIGIN      
        1 atagctataa aaaatataaa atacaactat taaaaaggag catatcatta taattttaaa
       61 aatcataaat gatatactcc taaaaatcta cttaacttat attaaattta ttttccaaaa
      121 tatctattta ataatcctaa gaatgcttta ccatgtctag cttcatcttt acacatttca
      181 tgtactgtat cgtgtatagc atctagtcct aattctttag cttttttagc taattttaat
      241 tttccatcag ttgcaccata ctcagcatca actctaactc ttaagttttc ttttgtatct
      301 gcaactacaa cttctccaag aagttctgca aatttagcag catgttcagc ttcttcaaga
      361 gctattctct tataagcttc tcctacttct ggataacctt ctctatcagc ttgtctactc
      421 attgctaaat acattcctac ttctgtacac tcaccaacaa agttagctct taatccttcg
      481 attatttctt catctacacc ttgagctact cctattctgt gctcatcagc ccaatttttt
      541 tcgcctttca tttcttcaaa tttatcagct ccaactttac atactggaca ttgtgctggt
      601 gcagcatctc cttcatgtat atatccacat actgtacaaa caaatttctt cataattact
      661 ttcctcctaa attttatata tcaaattaaa ttttttattt attaagttag tctatatata
      721 cccgcaccat atacctttaa acaatttttt taaaagtttt ttctaaatac ttattttccc
      781 tgtgtcaata atatgtttct ataataatgt ccctttaatt ttatcaattc tttatgattt
      841 cctgattcca caattctacc attttctaaa actattatat tatcacaatt tgatatggta
      901 cttaatctat gagctataat tatacttgtc tttccataag ttaaattatt tattgcttct
      961 tgaattttag cttctgttgc tatatctatg ttacttgtag cctcatccaa aataagtatt
     1021 ggagcatttt ttaatactgc tctagcaata cttattaatt gtttttgacc ttcacttaaa
     1081 tcttcacccc tatccgataa tacagtacta tatccttcac gcattttaac tataaattta
     1141 tgtgcatttg ctattttact agcatatata acttcttcat cagtagcctc cagatttcca
     1201 taccttatat tttccataac tgtatcattg aatatataac cttcttgtgg aactactcca
     1261 aaacaagttc tcaaatagtt tctgttaata gagtatatat ccttaccatc taaaaatata
     1321 cttccttcat ctatatgata aaaatttgta attagattta taatcgttgt ttttccactt
     1381 cctgtctctc ctatgatggc atttgatgtg ccagctttga tttttaggtt tatattatct
     1441 aaaattttct tttttgaatc ataagaaaaa cttacatctt taagctcaat ttctcccttt
     1501 acaaaatgag agtttaaatt accatcttta ttatcgttaa tctcactttt aaacatgtta
     1561 tgcacttctt gctcttcttg ttcatctata atttcaaaaa ctctttcaca tccagctatg
     1621 gctgaaagta atgtattaaa tagatttgct aattcattta aaggtcttgt gaattgtctt
     1681 acataactta aaaaactagc tataacccct atggttataa tatctttaga tgcaagaaca
     1741 cctcctataa tacatatagt tgcaaatcct atattactaa tcacattcat aataggcatt
     1801 aaaagagaag tgtaaatttg cgatttaagg gaaatattta gaagtctatc attgacttct
     1861 ttaaaatcat ttacagattt tctttcataa ttaaaagatt ttactacatt tatgtttgat
     1921 atcatttctt caatatgtgc atttagtttt cccaactcaa cttgttgttg cttaaaaaaa
     1981 actctagttc tctttgcaat aaaattactc acaaacatta ctatcggtat taatattaca
     2041 ctgcaaaatg ttaaaattgg gcttaataca aacatcataa taaaagtcat caatatgttt
     2101 aagattcctg taattaactg tactaatgaa tttgatattc ctgtactgat attatcaaca
     2161 tcatttgtca ctctactcat aatatctcca gtagaacttt tatcaaaaaa ggttattggt
     2221 agatttttaa attttaaaaa taaattactt ctcatttctt taactatact ttgagataat
     2281 ctagctgtac atatcccctt agacagttta actgaaaact caattatata aaatgaaagt
     2341 aaaataatta ttccttttaa gaaaatggat tttgaataag tatcgatatc tattatgtca
     2401 actacctttc caatagtata tggtattaca gttgatataa ttgaatcaat tattactagc
     2461 aaaaaaataa atctagtaat cttcttatct tttttaaagt acataaaaat tcttttaaac
     2521 gtttttttaa aatctttagg cttttctttt ttctttaatc tactgacctt ttgatttcca
     2581 gtacttaagt tcaatatact tcctccttaa tttacatctt cacttactaa attctgcaac
     2641 gcaaaaattt ttttataaat tggagaacta accagtaaat cttcatgtgt agcaaatcca
     2701 accacctcac cctcatccaa gactaaaatc ttatcacagt caattataga actaatcttt
     2761 tgtgaaatag ttattacagt acatttctta ctttcctttt ctcttaaatt acttatatac
     2821 tcttttatat tctttcttat aattgtctct gtattaatat ccattgcact aaaacaatca
     2881 tcaagaatta atatttgtgg ttttttggct atagctctag ctatagtaat tctttgtctc
     2941 tgtcctcctg ataaattttt acccctttga aataaaacct catcataaga attttcgagt
     3001 ttagttataa attgatttgc atttgctatc tcacatgatt ttataacatc ttctctactt
     3061 ataccctcat ttcccataac tatattttct agtatacttt ttccaaacac actgctattt
     3121 tgaaatacta cacctatttt ttcccttaaa ctttcttcag aaatactgta tatactacgt
     3181 cctccaatct taatatcacc tttatcaatt atatataatt tttgaattaa atttactaaa
     3241 gaagtctttc ctgaaccagt ttgaccaatc acaccaatat tttgaccttc ttctatttta
     3301 aaggatacgt tttttaatat tttttcatct gtatttttgt tatacgaaaa atctacattt
     3361 tcaaaaatta tatcacaatt tacaaacttt aaatcttttt ctccaacatc taaactttct
     3421 tctctctcaa ttacttcttc attctcatca accaaaacct cacttattct ctcataagat
     3481 gcttttgccc taacaaaaac attaaaaaca tgtgaaatca ttataagaga tgttaatatt
     3541 ctagtcatat agtttacata agcaactata atcccaattt taatttctcc tgaataaatt
     3601 aaatgccttc caatcagtaa tattattatt atggaaacat taatgattat tgtaattgtc
     3661 ggtctaaaaa atgacatcat agttgtagct tttctactat ttttaaaaag ttcatcattt
     3721 aactcttcaa agctttggtt ctcataatcg tatctacaaa atagtttaac tacttttatt
     3781 ccacttaaaa actgtcttaa ttttgaattt actttatctg tactttcttg tacattttta
     3841 aataatctat accctatctt catattaagc aatattataa taaatataat taaaactgca
     3901 attagaataa ttattgacat ctttaaattt aattttatag ctataattaa acttcctata
     3961 cacattattg gtgactttat aaaaactctc atcattccat taacgaattg ttgcatttga
     4021 ttgatatcat tagtaattct tgttattaac gaagctctac caaatttatc tatttgtttt
     4081 aatgagtatg tatttatctt ttcaaataca tcctgtctta aatcataaga aaaatgctga
     4141 gaaactcttg ccgagattac acttcttatt attgcaaata ttgcacctaa aaaagttata
     4201 cctatcatta tagctcctgt tgatagtatg tagtctaggt tcttatttga aacccctata
     4261 tctattattt ttgccattat agttggttga gctaaatcac aaaaagcttc caatgttaaa
     4321 aaaaatactg ctgttagaaa taatactata tattttttta tgtatttttt aaaatacttc
     4381 attcaatcat cccttcatag aattttctca ttaccaaaag tatatcataa tggctaattt
     4441 aa
//
LOCUS       DAMJVX010000237         4345 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.244, whole genome shotgun sequence.
ACCESSION   DAMJVX010000237 DAMJVX010000000
VERSION     DAMJVX010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.244"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(663..914)
                     /locus_tag="PC407_003417"
     CDS             complement(663..914)
                     /locus_tag="PC407_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498825.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(1160..1315)
                     /locus_tag="PC407_003418"
     CDS             complement(1160..1315)
                     /locus_tag="PC407_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDF2498826.1"
                     /translation="MALDVKIEVKIIKDVVILNIIHWKKETSLLTVKIARGQGDLSYV
                     KYFTNES"
     repeat_region   2532..3489
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2532..2560
                     /rpt_unit_seq="ctttacataccacttagttaatataaaac"
BASE COUNT         1516 a          646 c          403 g         1780 t
ORIGIN      
        1 ttatatcgcc tcttcaattt gagccccatc attatataaa attaacctac ttgatatctt
       61 aatttaatat atgtacacta ttcatatctt aatagtcaat attattcatt tttaatagct
      121 ctttttagat gtcagaatta ttatattttg aaatttatta aatttttgca ttaaaaaaac
      181 tagaaattaa tttaagtctt gaattactta taactttcta cgtatctacc tttatattct
      241 tactctgatt ttatatctaa tattaactct tcatcaaagc tattaaatat acctctaatt
      301 gagtcaatta tttcacttac ttcaccttta atttttatat taacttcaac catttcaaat
      361 ctcatctttt tatcttataa tttctataag tttttttatt tattgtgttg cttactttga
      421 ccagcacttt tttatttaat agtctaaatg atatttttta ccagttttaa tatccttaaa
      481 tactatattt actaaaaact tataatttct tttaatacat tcttattact aataacaata
      541 ctcaacaatt ttatcgcctt ttttcaaata tgatttaact tttttattcc taattattta
      601 aaaatagttg tccaattttc ttctatgaga aagtgctaag tattagcaac ttctctattc
      661 tcttaacttt ctactgtctc aactttcttc atagctatat taatagctac ttctttagct
      721 acttcattga actcttccca tcttctccta ttttcttcat cagatatacg tggatatact
      781 atataaactt ctgtgttttg attagctata attttttcat catattctat atttttttct
      841 ttatcatatt tgtatggttt aaattgttgt agtattttca taacatcacc ccttgttata
      901 aaatatgaac tcataaactt gtcttatact tatttaattg ttacaatttt tacttaaaat
      961 cactttatat tctatatttt aaagctaaat cctttacaaa agtaacatat tcttctataa
     1021 gtttcttatc ctcttatata gtatctagtt tgtttaactt atctctttta aacttacata
     1081 cgccttcttc tgccattcgt cttttattaa ttgttaacat tgtttcaaga gacacaccaa
     1141 aactcttgtt aagaagctct tagctctcat tagtaaaata ttttacataa cttaaatcac
     1201 cttgacctct agcaatttta actgttaata gacttgtttc ttttttccaa tgtattatat
     1261 ttaatataac aacatctttt attattttaa cttctatttt tacatctaat gccatattat
     1321 tgctttatta atttgtatcc ttaaatctta aattttctat taatacatct tctatacagc
     1381 ttagtttctc tcgcttaatc ttgaaatata ttttctctaa attgtcaaat tgttcccaag
     1441 ttttatcaat atctaatatt ttgcagtgtc tatttgcaca tctttcgatc caaaggtata
     1501 attgactaga atgttttgaa atcaactgac tattagtagg ttagttctta aattctttca
     1561 aatactcgaa ttgtaataaa acataattta cattctcttt atttctattg aagttagttt
     1621 atatattgtt tacatcattt tcatatatat ctgctagttg ctatgcattt aaaattctct
     1681 tattatttct ttatattact tgtaaattat tcatcctcat catcctctta attaaatttc
     1741 tatcttagaa atttaatttg taaaaaaatt ttctatatca taatcaagta tcttagatag
     1801 tttataggta acatatcaac tctaattgaa tattacccct tcctgtactt aaaataagtt
     1861 gatgcatttt taaaacctaa ctcttttgca acactttgta agtataaatt ttatgtatgt
     1921 cttctttttt ataaaatcat agtgtatctt tttcatatat tcaccttaat tgctatattt
     1981 caataacaat atttccaaaa cagaaatttt tcgaggtaat tatgtataga tgtaaatcaa
     2041 agaattaaat atactaggga aaatatgaat agttctcaaa aagaattgct tgaaaaaaca
     2101 aatataaata caagtgttat gaatagaatc tggtgaaaga ccaacaagag acgatgggtt
     2161 aataatattt gctaaaattt ttggtttata tactgattat gttcttggtt tatcagatac
     2221 agaaaaacta aatattggca attaaaactg aattgattgt attatatgtg tgatgaatat
     2281 aaatatagaa ttttttgaga agcttgaaat agcttagaaa aaataagaga ttcactcaag
     2341 aataagttac taaagattaa aaaataacaa aagtatctac taaaggatat gaaaatggtt
     2401 taagagagtt aagtctggaa atatttatgt gtattcttta aataaaaatt tattttttaa
     2461 atgataatta aatttttact gatatggcta tgcttttaaa agtcataacc ttattttgat
     2521 attagatctg tctttacata ccacttagtt aatataaaac taaatatagc ttgtaactcc
     2581 tttgatgcac ttaaaagctt tacataccac ttagttaata taaaacgaat ttagctatag
     2641 aagcatcaaa ttgtggcaca gcactttaca taccacttag ttaatataaa acctttaaag
     2701 acaaaacgtc gtataattaa atcatagcaa ctttacatac cacttagtta atataaaacc
     2761 ttataattct ttaagtacta gactgagcaa tttaatcttt acataccact tagttaatat
     2821 aaaaccatcc cattcagcaa caacagtcgc taaatcttct gcctttacat accacttagt
     2881 taatataaaa cttttagtat attttttagg tctgccctct ttaaaactct ttacatacca
     2941 cttagttaat ataaaacacg aggttatagt taattttgtc gtgggagttt ataactttac
     3001 ataccactta gttaatataa aacaatttat atatatatat atatatatta aagtcagacc
     3061 cctttacata ccacttagtt aatataaaac ttgttgttca gcattagcaa tcatatcatc
     3121 tattcctttc tttacatacc acttagttaa tataaaacct gtaccatcag agacagtcat
     3181 taaaagattt ataaacttta cataccactt agttaatata aaacttataa attgtctctg
     3241 tacccatttt attcaatgtc gtactttaca taccacttag ttaatataaa accatgaaaa
     3301 aatgggtaat tcaaactctt ttattttctt tacataccac ttagttaata taaaacgttg
     3361 gagaaaataa tccttcttat gatttgccaa gcggctttac ataccactta gttaatataa
     3421 aaccagcgga tgaattattt atgttaattg gagatggaga ctttacatac cacttagtta
     3481 atataaaacc ccaaaataaa ttgagtattt ccaacactta catctataca attctctcaa
     3541 atttgcagtg aaccatgagt agtgcaaatg ataacattta tcacataccc tcaatgcctt
     3601 atatttcaac tgttaggccc tattttatca aaaatatcgc tcactgcaaa actatttata
     3661 attttataat attatgaata tattattttt aaatatctgt accaatttgt agtataataa
     3721 aatcaagaaa ataatttact cctataaaga gtgattattt ttaagtgaaa atattatttt
     3781 tttaaatcat ccttattggc gtttgggaga tttttatttt atcataaata taaactgata
     3841 taacaccagc tgacatgctt gctgctttcg attattattc ttgctacaat tattataaca
     3901 tataaaactt acttattttt accaaatatt ttatacaaac aataaatatt aacttatgaa
     3961 atatctactc atttacagat atttcataag ttaatagctc tataataaaa tttttatcat
     4021 tgtttataaa aaattttact ttgcattttt ttataaacaa cagttttatt tataatgttt
     4081 tgtggtataa taaaagcaag aagaactaca atctattttg cgttagagtg gagttcatca
     4141 taaacagagt ttattttttg gatttaaata taaatttaaa ttcaactcgt aagtcactct
     4201 ttgtgctgaa ctatacccac tgttttatac agttaaaaca tgtaaaagtg gttataaagt
     4261 actagtgtca atcattagta ctttttttat tatattcata taattaatag ctatatttta
     4321 aagtttacta tctaaaaaat agcac
//
LOCUS       DAMJVX010000238         4311 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.245, whole genome shotgun sequence.
ACCESSION   DAMJVX010000238 DAMJVX010000000
VERSION     DAMJVX010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.245"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(732..1142)
                     /locus_tag="PC407_003419"
     CDS             complement(732..1142)
                     /locus_tag="PC407_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498827.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(1358..2740)
                     /locus_tag="PC407_003420"
     CDS             complement(1358..2740)
                     /locus_tag="PC407_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2498828.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKSGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIEQSNYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(2751..3110)
                     /locus_tag="PC407_003421"
     CDS             complement(2751..3110)
                     /locus_tag="PC407_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2498829.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(3315..4094)
                     /locus_tag="PC407_003422"
     CDS             complement(3315..4094)
                     /locus_tag="PC407_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDF2498830.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         1516 a          702 c          400 g         1693 t
ORIGIN      
        1 tatctatttt tttctggtat tcttatctac tattatccgt atatttattt tatgattact
       61 cttttaacct accccctata ctttatagta atatgttttt aacctttttc attattccca
      121 atatgcttta taactacaaa tactctcccc ccttaaaaat aataataaaa ttaatcaata
      181 aatttataat catattatta ctctatgtat taagcaatta ctatgccaat aaattttgat
      241 ttttttaaac aaatcttaaa aatcctaaaa tgctaatttt ctttaacttt taatgaatct
      301 aatagataat atttaatttt cgttatattt ttcagaatgt ttcaattatt atttttaaga
      361 aaaaaaaata atgttaacat ttcttaacat acattaaaaa atgttaacat tataaatttg
      421 gtttaatact tttactttga ctcgatttac ttttattcta cttcttactt tatttttaac
      481 ttagacttta tttttttatt acttatttga gtcttattta ttttattctc actccatatg
      541 ttcaattaca tttgtctaaa ttctttgata cttttattac ataattttaa caagcttaag
      601 tgttatttgg gtagtgcagt tgataattat ttaaataatc atcaactgca tgtttatatc
      661 attacagtag cttaatgtat ttaatcatac ttacttagga ttttgaaata tcttagagat
      721 tatatttaac attagtcaat tgctgcataa ttgtaagcaa ttgaaatttg agacccatct
      781 tcattttcta tagtgatagg cttcataatt tttaatcctt cagtttttat cttttctaat
      841 tcttgttcca ttttcttaat tgtttcctca aggtcttttt gacttacatc tttgactgca
      901 ttagggtctt tagcatagtc ttttaattct tgaatatgtt tagcatactc ttcataagta
      961 taatgctcaa caccatactt ttccaattca gtagggtcag ttattcctga ttttattaga
     1021 gattcaagtt taggtaaagc aatattagat gaatcatttc catttgcaaa tactgctcca
     1081 gttcctccta gtaccattgc agataccatc attgcactta taagggattt ttttattttc
     1141 ataaccaatc ttcctttctt agagatttat ttgggtagtg tagtttaaaa tgactattgc
     1201 aatagttatc aaccacatct ttagactacc atagtagtca tatttgtgtc aagtcttttt
     1261 aaaaaacttt tcaataaaaa atactatctc aaaacaaaat taattatttt gagatagtat
     1321 tttaattcta aatcttttat attactaaga tttatggtta ctctatccat gttctatatt
     1381 gttctttatt aactgctaac aaattccctt ttcttgatat gacaaagcga ttatcattat
     1441 agttactttg ctcaattgaa acttctcctg aatcagtata aagtacatcc ttactattac
     1501 tagatttatt tacatacgtt tcttcactat tctcagaatt agttactttg ctcatagatg
     1561 attcttcatg ctctctaaat tcaattacag atacttttgc cgtactaaaa tgagtctctc
     1621 catcactttt agctttacta acaagaatat cagattctac tttgggagaa tataaatcag
     1681 ttgatttact acttattgaa aatccacttt tatcactttg ttcatttttt gttaaaccaa
     1741 aagattcaac ctcattaatc tctttatttt ctgttgcaaa aacaagacct gtcccacaaa
     1801 ctaatacagt tgaaactaaa aaagtaacat aagttgattt tttaaatttc ataatattaa
     1861 ctattctctt ttctattttt gtctttccaa atccactaca tattgtcatt gaaaatggat
     1921 tattctcctc tgctagactt attagtgtgg ttgcataatc agccctatat tcttcaccaa
     1981 gtatactcat aaccttttca tcacaactaa attctatgtc tttatttagt aaaaaaaaca
     2041 tcacccaaac taagggatta aaccaatgga caacaagagc tacaagcata aataacttta
     2101 acagattatc aaagctatta atatgaacca tttcatgagt taaaacaaag tctaaagttt
     2161 gattattagc ataattcatt tgctttggca aaataattcg tggttttaaa ataccagatg
     2221 taagtggtgt cctagtaaat tcagatatgc aaacaataaa tcttctaaaa cggctattct
     2281 tacacatcca ttttttaatg aactcatgat tttttatatg aattgaagtt ttaaaaagat
     2341 taattatttt aaaatatcta aagcataaaa gtatgctaaa cataatagta actataaaag
     2401 acaaaatgcc caacatttcc aacatcataa tattttcaat attagacatt atttcaaatt
     2461 cgtcagaatt gtttttacct gtagagtcac tagctttctt aagagtataa ttttctgttg
     2521 atgtattacc caattctaca tcaaaagtcg attcaattat actataaata ctaaaaccag
     2581 aatttaaact tactggtatt agaagtttaa ataaaacaat caaccacaat aatacaaata
     2641 cctttttagg taatttttta aataaacatg ctcttataaa aataactatt aaaagtaata
     2701 ctccagaagt aaaactcatt tctaacaatt taatgaacat aaatcttacc tcactttaat
     2761 ttttcaacaa tttgtttgag ttttgtaatc tcatcacttg aaagattttc tttattaata
     2821 aaagctgaaa gaaatttttc tgaagaccct ccaaacattt tattaattaa agtttctgtt
     2881 tcctgttttt gtacttcctc tttgcttatt aaagctttac aaacaaaatt tggctcaatt
     2941 ctctctattg caccttttga cactaatttt tttataacgg tatacgtagt gtttctattc
     3001 catcctgttt cttcttttaa aatttttact aactctccag ccttgctttc tcctctctcc
     3061 catagaactt ccattacttt aagttctgaa tcaaataatt ttatctccat ataaaatcgc
     3121 tcctcaaata tagttatcac aatagtcatt actataataa aaaactatca tagtagtcta
     3181 tactatgtca atcatttatt ttttaataat aaaaactgtc tttttaaaaa attcttataa
     3241 aagacagttt tatcaatgtt atactttaat gtttaattaa agccatattt tatttaaatg
     3301 tataactatc tatcttagta catagtataa gatttttctt tatctaatag tatatcctct
     3361 acatctgggt tttcttttac taattgtcta taaaaattac aattagtaac acctacatat
     3421 ggaattagcc atagtagacc tattccaaga gtagctattg aaagtatata ccatcctata
     3481 aaactaaagt ttagtacaaa taatttaagt acatttcctt tcatcatatc taaactagca
     3541 cccattgatt taaatacacc catattatcc ttgtcttcac aaattaagaa agtagcttga
     3601 gaaacatata atagcaatat ataaaccggt aacataagaa caagtattat aagaactaga
     3661 gttattccag tagaaataat agtccctatt ccaacattat cacttgataa atattcaatt
     3721 gctccactag taagtaaaac tccaaatgat aaaaacgctc ctataattgt gattataaaa
     3781 gaataaatat aaacaataat tgattcaaat aaataaaaac caaaacttct taaataaatt
     3841 ctcccactta caaagatatc tcctatcttg actttttcat ctttagtaat tttcaagaac
     3901 atacatgtaa atcctgctga aacgcaaaaa gctattacag ccactacaat agtagtcaat
     3961 ggttcaaaag gtaatcctac aattgatatt aatactatca atgcaatata tactgccata
     4021 gaaattgcac atattcccca gtttccagat aactgagctt tagatactcg ttttaattct
     4081 tgccttgata ccataaaaaa cacccctttc attttataaa aaaactttta aaaatataaa
     4141 taatctttta acgtataata ttaatatatc atattttcga ctatataaga tacaattatt
     4201 caccatgttt tatcaagata tgccataatt gtattactag ttttatatag tcaacattac
     4261 caaatacatc aaataaattt gattaattat tcaacaagta ataaaataat t
//
LOCUS       DAMJVX010000239         4202 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.246, whole genome shotgun sequence.
ACCESSION   DAMJVX010000239 DAMJVX010000000
VERSION     DAMJVX010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.246"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            264..2363
                     /gene="nrdE"
                     /locus_tag="PC407_003423"
     CDS             264..2363
                     /gene="nrdE"
                     /locus_tag="PC407_003423"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDF2498831.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYLKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2376..3347
                     /gene="nrdF"
                     /locus_tag="PC407_003424"
     CDS             2376..3347
                     /gene="nrdF"
                     /locus_tag="PC407_003424"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDF2498832.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKKMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3423..4049
                     /locus_tag="PC407_003425"
     CDS             3423..4049
                     /locus_tag="PC407_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2498833.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIIEIPEQKFIMLKGSGDPNSKEFI
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         1688 a          449 c          775 g         1290 t
ORIGIN      
        1 ttgataaaaa tttctttaaa taaaaataat gtcaattata aagtaataaa agtttagata
       61 taaaaaaatt tgtgtatata ctatatgtag tatataatag ataaagcact gactatatgt
      121 agtgcttttg tttttgaaaa atacttattg catttctata attatagatt aattttggga
      181 tttcaactga taagtaccaa aagaaataat aagcaaatta agtgaaatta tggcacaaga
      241 ttatgttaaa aggacggtaa ataatgaatt ggatagagct taacaatcaa gtgataatta
      301 aaaatgaaca aggtaagtac caattggaaa aggataagga agccttaagt agttacatag
      361 aagaatttat acaaccaaag ttgagaaaat ttaataacct agaagaaaga ttgaagtatt
      421 taatagatga aggttattat ttaaaagaag taataaatca gtatagcata gatgttatta
      481 aaaatatata tgatatgatt gataaacata agtttgaatt tcaatcatat atgagtgcaa
      541 ataagtttta tcaaaattat gcattaaaga gcaatgatgg aaaggaaata ttagaaacat
      601 ataatgataa agtgttaatt gtagctttga cactaggaaa tggcgataaa gatttagctt
      661 taaacttggc agataaaata gtaaaacaag aatttcaacc agctacacct acttttttaa
      721 atgctggaag aaagagggct ggagaaatgg tatcatgttt tttactatct gtagaagata
      781 gtacagaagg tatatcatat gctatatctt catcaaatca cttatcaaag attggtggag
      841 gagttgcact aaatttatct aaacttagag catctggcga atctatcaaa gatatagaag
      901 gtgcagctgg aggtgttgtt ggagtagcta aaatgttaga acaatcattt agttacttta
      961 atcaaatggg agctagacaa ggttctggtg cggtgtacct tactgtattc catcctgatt
     1021 ttgaattgtt gatggatact aaaaagataa atgcagatga aaaaataaga cttgctacat
     1081 tatcattagg agctataata ccagataaat ttatggagtt ggcagaaaaa aatgaagtag
     1141 catatgcatt ttatcctcat actgtgtata agaaatatgg agtttctctt gatgagatag
     1201 aaatggatga gtggtatgat aaattggtta atgatagtga tataagaaaa aaagaaataa
     1261 atcctaggca gatgttgaca aaaatagcac aaatgcaaca agaaagtgga tatccatatg
     1321 tagtgtacat agatacagct aatagagaac atacattaaa agatgttgga attattaaaa
     1381 tgtctaactt atgttgtgag atatttcaat atcaaacacc ttcagaaata gaggggtatg
     1441 gaggaaaaaa tgaatgggga caagatataa gttgtaacct aggttcttta aacatagcca
     1501 atgtaatgga taataaaact atagaaagta cagttgaaac agcaataaga gcactgtcat
     1561 ttgtagcaga tagcacagat ataaaaccag tgccaacggt aagcaatagt aacagcaagt
     1621 ctcattctat aggattagga gctatgaatt tacatggata cttagtaaga gaaaatattt
     1681 tatatacttc agatgatgct atagattttt ctaatgtatt ttttgcaatg gttagatact
     1741 attcaattaa agcttctatg aaaatagcca tagaaagaaa tcaaactttt gaaggatttg
     1801 ataaatcaga atatgttaag ggaagaaata gcaaggtttt atcaaaatac tatgaacaat
     1861 cttatctacc taagtcagaa aaggtaagag cattatttga agggatttat ataccaacaa
     1921 aagaagattg gacaaaatta ttggatgaag taaaagaaaa agggatatat aatgcatatt
     1981 taatggcagt agcaccaact caaagcatta gttatgttca aaatgctaca tcaagtataa
     2041 tgccaattac tgagccagta gaagtaagaa catatggaga ctctactaca atatatccaa
     2101 tgccattttt aacaaatgat aatatgttgt attatcaatc agcatacagg atggatatga
     2161 gaaaggtcat agatttagtg gcaacagttc aaaaccatgt tgaccaaggt atatcaacaa
     2221 cactgtttgt tacagatgaa aagacaacta gagatatagc tagacattac atatatgcct
     2281 ataaaaaagg tttgaaaagt ttatattaca caagaacaaa gatgacaaga gatactcatg
     2341 agtgtctagt gtgttcagta tagggagaga aatatatgac atttaattta cataaaatac
     2401 ataatgcagt taactggaat agagaagaag atggatttac acaagctttt tgggaacaaa
     2461 atgtaaaaca attttggctt cctgaagaaa tatctgtttc taaagacata aaggtatgga
     2521 atgaactaag taataaagaa aaggaattat ataaaaaagt attgggtggt ttaacattac
     2581 tggatacaaa acaggggaat aatggaatac catctatgat gagtttaaca gaaaacctac
     2641 aaagaaaagc tgtcttgtct tttatgggga ctatggaaga aatacatgca aaaagttatt
     2701 cttctatatt tatgactcta ctttctaatt tagaaataga tgaattattt gaatggatag
     2761 aaacagaacc aacattacaa agaaaggctg atttagtttt agctcaatac gaaaatacta
     2821 caaatcaaga gggattatac ctatcaatgg ttacaagtgt atttttagag agtttcttgt
     2881 tttattcagg attcttttat ccattgtatc tatctggtca aggaaagatg gtagctagtg
     2941 gagaaataat atcgcttatt cttagagatg agtctttaca tggtaagtat ataggattgc
     3001 tagcacaaga aatatacgat tcatttgata aaatggataa gaagatgtta gaagagaaga
     3061 tgtattctat actttatagc cttatggaga atgagataga gtatacaaat gttatataca
     3121 gagaaagtgg gcttgaaaaa gaggttgtga actttttaaa atacaatgcg aatagagctt
     3181 tagaaaatct agggtttgag aagctatata ctgtagatgc tataaatcca attgtattaa
     3241 atggtcttag cactgaaacc aaaacacatg atttcttttc aactaagggt aatggatacc
     3301 aaaaaggagt gtatgaggaa ttagaggatg aagattttat aatataattt ataattatga
     3361 cacactgttg tcacttttag cgtgataata tcaatgtata aaatatatat ggaggtattt
     3421 ttatgaaata tgaatggaga aaaagagata aagagttata tttacctaaa aagaatcctg
     3481 taataattga aataccagaa cagaaattta tcatgttaaa agggagtgga gacccaaatt
     3541 ctaaagaatt tattgaagca ataggagtac tttattcttt ggcatatgca ataaaaacca
     3601 tgcctaaaaa aggaattgta ccagaagggt attttgacta tacggttttt cctttagaag
     3661 gtatatggga taagggagaa aaaagtaaag gcttagatgt acttattaaa gaagatttaa
     3721 tttacactat aatgatacgt cagccagatt ttgttacaca agacctgttg gataaagcta
     3781 ctgatatagt aaagaagaag aaacctcata atcttcttga aaaagttagg tttgggtcta
     3841 ttaatgaagg gctatgtgtt caaatgcttc atataggttc atatgataat gaaccagaaa
     3901 gttttgacat tatgaacgaa ttttgcaaaa aaaataaact tataagaaaa tcttacacac
     3961 atcgtgaaat ttatataagt gatgctagaa aagtatcgcc tgaatcattg aagacggttt
     4021 tacgttttga agtagaaagg gaagtataaa tattttgaat aatttataag agttataaaa
     4081 agtgaagtct gttgagactt cattttttga tgcaatttta aaattaaatt tgatgtttca
     4141 taatatttta aaaactttct acttttatta gtgtgaataa ctgttgctat taaataataa
     4201 ta
//
LOCUS       DAMJVX010000240         4168 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.247, whole genome shotgun sequence.
ACCESSION   DAMJVX010000240 DAMJVX010000000
VERSION     DAMJVX010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.247"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            167..2479
                     /locus_tag="PC407_003426"
     CDS             167..2479
                     /locus_tag="PC407_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2498834.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKENNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDTTSNKVNYISDELD
                     TTSNKVNYVSDELDTTSNKVNYVSDELGMALHKVNCVSDELDMTSNKVNYVLDELDIT
                     SNKTDYISNELYNLSNKSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIKFKVCF
                     DMFMTDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFME
                     LAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNLVAWEDKKFET
                     PSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKK
                     SIAQAYINQRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHG
                     NPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            2567..2902
                     /gene="spoIIAA"
                     /locus_tag="PC407_003427"
     CDS             2567..2902
                     /gene="spoIIAA"
                     /locus_tag="PC407_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HDF2498835.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            2916..3344
                     /locus_tag="PC407_003428"
     CDS             2916..3344
                     /locus_tag="PC407_003428"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HDF2498836.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            3367..4131
                     /gene="sigF"
                     /locus_tag="PC407_003429"
     CDS             3367..4131
                     /gene="sigF"
                     /locus_tag="PC407_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HDF2498837.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
BASE COUNT         1710 a          404 c          795 g         1259 t
ORIGIN      
        1 ttagtatgca aaattctgtg aagcaggaac actgtgactt tagtcatggg aggttcagaa
       61 aatatagcta tgttttatat actgtagttt tgaacataga aagtttaata aattatagta
      121 tatattaatt tttaataagg taagaaagta gaaagaagga tattttatgg atataataaa
      181 aaaattaagt catggatgta cattagattg tcatgattgt tgtaaattca atgtttatac
      241 taaagaaaat aatgtagtaa aaattgaagg agataaaaat catccttata caaaaggttt
      301 tatatgcaaa aaagggatgg ctcatttaga tagattaaat cataaagata gaataaagac
      361 tcctatgctt aaagttgatg gagtatggaa agagatttct tttgataagg ctatagaaat
      421 aatggcagaa aagcttacat actataaaga aaagtataca tcaaagtctg tcatgcatta
      481 tgaccaatat ggaagtgggt ctgtgctaaa atacatagga gatatatttt ttaattttta
      541 tggaggtgtt agcagacata agggtggtcc ctgttggagt gcagggatgc atgctcaaaa
      601 atatgatttt ggagtagcta agagtcatgc tatagaagat atgttaaata gtaaaagtat
      661 atttgtgtgg ggaaaaaatc cagcatatac tacaattcat actatgcaaa taattaaaaa
      721 agctaaagaa aaagggataa agatagtagt gattgacccc atatatacaa aaacagctca
      781 aatagcagat aagtatgtac aggtaaatcc tggaacagat ggagctttag ctatagctat
      841 ggcaaaaatt atagtagaag ataaattata tgatgaagaa tatataaact cgtatgttat
      901 tggttttgaa gaatataaaa aatatttatc ttcattggaa ttaagttttt taattgatga
      961 atgtggtgtt aaagagaatg atataagaga gttagtggat ttatatacta acaaatattc
     1021 aagtataaat gttggatatg ggcttcaaaa atataaaaat ggtggaaaca caataagagc
     1081 gatagatgct ttaggtgcta taactggtca aataggattt agtggtggag gggtaaacta
     1141 tgctaataaa gtttatccaa gtgttataaa ttctgaccct tacaatagcc aatcatatgg
     1201 tgaagatagg gagttttatg taagtaatat cagtaaattt atagaagaat ctttaaaaaa
     1261 tacatcaaat aaagtaaatt atgcatcaga tgaacttgat acaacatcaa ataaagtaaa
     1321 ttacatatca gatgaacttg atacaacatc aaataaagta aattatgtat cagatgaact
     1381 tgatacaaca tcaaataaag taaactatgt atcagatgaa cttggtatgg cattacataa
     1441 agtaaattgt gtatcagatg aacttgatat gacatcaaat aaagtaaatt atgtattaga
     1501 tgaacttgat ataacatcaa ataaaacaga ttatatatca aatgaattgt ataatttatc
     1561 taataagtca atcaaagata atattcctat aaagatggct gtgataacta aaagtaatat
     1621 gctgaatcaa ctgccagatt tagtagaatt ggagagggtg ttttcaaaaa ttaaatttaa
     1681 agtatgtttt gatatgttta tgactgatac agctacttta tgtgacatgt ttataccgtg
     1741 tacaaataca ctagagagtg aagatataat ttatagctca atgaccaatc catatataac
     1801 ctataatgaa agagctgtaa agccagcaca taaattgatg gatgaatatt atttctttat
     1861 ggagctagct aaaaaaatgg gattaaatga ttatcctttt gtagaaaaga gaacttattt
     1921 agaaaaagtg attgaaccac ttaagcgttt tgacaaaaat ctggatatag aaaaacttaa
     1981 aaacaattac tttactatac ataaccttgt tgcttgggaa gataagaagt ttgagactcc
     2041 atctggaaaa tacgagcttt attcagaaag tataaaaaac ttgggaataa gtccaacacc
     2101 tgtatatata agcaataaat ataaagaaat agaagataaa aatatttctt ttagattatt
     2161 aactaatcat catgcagata ctttatttag tcagcatttt atggacaaaa aatctatagc
     2221 tcaagcatat ataaatcaga gaatggctaa aaaagtaggt attgaggaca aagatattgt
     2281 gatattgagg tcaaaaaagg caaaaataaa tgtacaaata aatatagatg atggagtagg
     2341 taattatata gtaaaaatgt atgttggttg gtggaagaaa catggaaatc ctaatagttt
     2401 aacagacact ggtatatcag attttggagg tcaggtaaca tataatgaaa gtatggtaga
     2461 aataatacga caaaactagt taaatttcat tgaaggaata aaaatataat tatagaattg
     2521 attaaaagtt actattttta caaaactatt gaaggaggga tttttgatgg taaattattc
     2581 cttagaacat aagaatctat acatagaatt tatgtgctca gaactagacc accatgtagc
     2641 caatgaaata agggaagaaa tagataactt attaagtgta aatcaagtaa aaaatgttgt
     2701 atttaatttt gaaaatatta attttatgga ttcttcaggt ataggcgtca tcataggtag
     2761 atataaaaag atttctaatg aagggggacg agtttcagta attaatataa gttcacgagt
     2821 taagaagatt tttgatttat ctgggttaaa taaaattatt ggcatttatg atacgtatga
     2881 agaagctcta agttctttgt agggaggagt atgtgatgaa taatattatg gaagttaaat
     2941 tttcggcaat atctgaaaat gaaagtttag caagagttat agtagcatcc tttgcagcta
     3001 agttagaccc aacagtagat gagttagtag atataaaaac tgctgtctct gaggctgtta
     3061 cgaatgctat aatacatgga tatgaagaag acagttctaa gtttgttttc ttaagatgtg
     3121 agatagaagg aaatactata aagattatag ttgaagatga agggtatgga atagaaaatg
     3181 tggagaaggc aatggaacca ctttatacat caaaaccaga acttgataga tcaggaatgg
     3241 gattcacagt aatgaaaagc tttatggacg atgtagaggt aagttctgta aaagataatg
     3301 ggactagaat tgaaatgact aaaaaaataa atgtgcctaa ataaagtcca aaagtgaggt
     3361 tttattatgg aagtaactgt tgccagagaa gaaaaaaaac ctcttcttag tcatgaagag
     3421 actctagaac ttattgaaaa agttcaaaat ggagatgaag aggcaaaaga aatattaata
     3481 tcaagcaatt taggtctagt taggagcgtt gtatctagat ttttaaatat aggatatgat
     3541 agagaagatt tatttcagtt aggttctata ggcttaataa aatcaatata taaatttgac
     3601 ccaaaattta atgtaaaatt ctctacttat gcagtaccaa tgattttagg tgaaataaag
     3661 agatacttaa gagatgatgg tatgataaaa gtatcaaggt cgttaaagca aatagctgta
     3721 aaggcaaaga tggaaagtga agctttaact aaaaaattag gtagagagcc aagtatagaa
     3781 gaattagcta aagcaataga tgtagaaaaa gaagatttag taatggctat ggaagctaat
     3841 ttcaatgttg agtatttgca aggagttatt catgaagagg aaggttctcc aatatgcctt
     3901 attgataaaa taagcatgaa aggagaaagt gaagaagaaa aagtagtgga taacatattg
     3961 ttaaaagaca tacttggaag actggataag agagagcgtc aaataatagt tctcagatac
     4021 tttgaagaca tgactcaaag tgaaatagga gagatgctca atatatcaca agttcaagta
     4081 tctaggatag agaagaaagt attgtctaag ttaaaagaat atatatcgtg ataatactta
     4141 tgatatgtag aataacaaaa tataataa
//
LOCUS       DAMJVX010000241         4119 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.248, whole genome shotgun sequence.
ACCESSION   DAMJVX010000241 DAMJVX010000000
VERSION     DAMJVX010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.248"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            118..1521
                     /locus_tag="PC407_003430"
     CDS             118..1521
                     /locus_tag="PC407_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2498838.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFMLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     TSLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1612..1852
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1895..2593
                     /locus_tag="PC407_003431"
     CDS             1895..2593
                     /locus_tag="PC407_003431"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDF2498839.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILKGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2616..3617
                     /gene="trpS"
                     /locus_tag="PC407_003432"
     CDS             2616..3617
                     /gene="trpS"
                     /locus_tag="PC407_003432"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDF2498840.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANAIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
BASE COUNT         1549 a          455 c          741 g         1374 t
ORIGIN      
        1 aactgttttt gaatatgcaa atattttgaa aaatatcagt ttaataataa tgaatctatt
       61 ttattaattt tgaaattaaa tttaaaataa ttaatagaga ggggaaatga aattaggatg
      121 gaaaagaaaa aaataggcct atggagtttg gtatttatga atgtatcagc tttatatggt
      181 ataagatgga tagctaagtc tacagcatca agctttggtt taggattagg tgcaatacct
      241 atgtggttat tattctcatt tatatacttt gtaccaggag ctttaatttg cgctgaatta
      301 gcttctacat atcaaaaggg tgatggaggt ctttacgatt gggttaagga agcctttgga
      361 gaaaagtatg gatttttagt ttcttggtta aactggacag ctaaaatatt ttggtattct
      421 tcatttttaa ctttcttagc agtaaatgtt tcttatacaa taggaaatcc agctcttgct
      481 gataataaga tgtttgtact tatattatct ttaataatat tctgggcatt atctcttata
      541 gcaactaaag gaataagttt tacaaaaata tttactaata caggtgcatt aggttcaaca
      601 ataccaagtg ctatacttat aatattttca tttgtagcag tatttatgtt gaaaaaacat
      661 gatatagctt ctacatatac aatagcttca atgactccaa aacttaatat ggatgcattt
      721 gttgctatat cagctataat gtttggttta gcaggagcag agactgctgc taacttcata
      781 acagaaatag ataagcctga aaagaacttc cctaaggcaa tattaatatc agctggaata
      841 gtagcttcat tatatgtatt aggttcaatt gcaataacaa tgataatacc accagaccaa
      901 attactgctt ctaaaggtgt attagatgca ctttctgctg tttgtacgtc tttaggaatt
      961 ggttcttggt tggttcaatt aatagctttt ggtatagctt tctcagtatt aggagcaata
     1021 gttctttata tagcatcacc aataaaaatg ttatttggta gtgttaaaaa aggaatattt
     1081 ccagatagtt tggtagaggt aaatgaacac aagatacctt ctaaagcagt tatacttcaa
     1141 gcaataatag ttactataat actattggtg actacattaa tgccttctgt tgatgctata
     1201 tacaatgttc ttgtaactat gacagcatta actgcattat tcccatatgt attattatat
     1261 gcttcttata ttaaactaag aaaagagaga ccagatgaaa ttagacctta tactatggca
     1321 aaatcaacaa gtacttgcct aggtttagca aaaatggttt tagttgtaac tgtagttggt
     1381 atattattat ctgcagcacc agttatgccg acatttgctg aaaatgttat atatgagata
     1441 gagatgattg gtggaggtct tctagttata ttatctggtc tatggttatg gaatagatat
     1501 gaaaagaaaa cagctaaata aaatgtattt taaaaatttc ttttggatat tataatatat
     1561 agttgacaat ttatagtgta aggaatatta taaaataaag aaattaataa attaaatcct
     1621 gagataagaa atagtaaata aatatatttt tgcacagaga attaaggatg ctgagaactt
     1681 aataaaaagt atttattgaa tgggtctttg aggagtaaaa tgaaatgtaa tttaagatta
     1741 cagagtattt gatatcgtgt atgcacgtta tagcatatat taagatatat atattcagtt
     1801 ttgagtgtat ataaatttag gtggtaccac ggaaaatata tccgtcctat tgatttagga
     1861 cggatttttt aatatagaaa atttaggagg aaaaatgaac gtaataggaa ttataggtgc
     1921 aatggatgaa gaagttagta ttttagttga tttaatggat attagagaga ctataaaaaa
     1981 agctagctta gaattttata agggaatttt gaaaggtaag aatgtagtgt tagtaaagtg
     2041 tggtataggt aaggttaact ctgctctttg tgcccagata ctaataagcg aatttaaggt
     2101 tgatgctatt gttaatacag gtgttgcagg agctttaaat gaaaaacttg atgttaatga
     2161 tatagtaata tcaactgatg ctatacagta tgatgtagat actacagcat ttggagaccc
     2221 taagggagtt ataccaagaa tgaagacttc tgtatttaaa gcagatgaaa gacttataga
     2281 tgctgcttac aaatcttctg tggaagaagt aaagactcat aaagtattaa aaggaagagt
     2341 agttacagga gataaattta taaattctaa agaattaaaa gaagaattag taaatgattt
     2401 tggtggatat tgtggagaaa tggaaggtgg agctatagct catgtatgct atttaaataa
     2461 tactccattc gtaataataa gagcaatgtc tgataaggca gatggaagtg ctgatgttac
     2521 ttatgatgta tttgtacatg atgctgccaa taactctaaa gacatagttt taaatatgtt
     2581 aaaatcaata taaatatata aggaggtaca aaaaaatgag tgaaaaaaaa gttatattta
     2641 gtggagcaca gccatcaggc aaaatgactt taggaaatta tttaggagct ataaaaaact
     2701 ggactgaact tcaagataat tatgattgct attatagtat agttgattta catgcaataa
     2761 cagtacctca ggaccctaaa gttttaagag cgaatgctat agaattactt gctcaatata
     2821 tagcttgtgg attagaccca gaaaaaaata ctatatttat acaatcacat gtcaaagagc
     2881 atgttgaact tatgtgggta ttgaatacta tgacttatat gggtgaatta agtagaatga
     2941 ctcaattcaa agataaatca caaaagagtg aagcaaatct aaatgcaggt ttattcacat
     3001 atccagttct tatggccgct gacatattgt tgtatcaaac tgacctagtt ccagttggag
     3061 atgaccaaaa acaacattta gaattagctc gtgatttagc aaataggttt aataatagat
     3121 tttcacctac ttttgtagtt ccagaaggat actacccaaa aggtggagca agagtgatga
     3181 gtttgcaaga gccaactaaa aaaatgagta aatccgattc aaatgaaaat gcatttatac
     3241 tattagcaga tgattcagat tctataaaga gaaagataaa aagaagtgtt actgattctt
     3301 taggtgttgt aaaatacaat gatgaacaac caggtattaa aaatctaatt gatatatatt
     3361 ctaatttaag taaaaagagt gtagaagaga ttgtaaacat gtatgaagga aagggttatg
     3421 gcatatttaa agaagatgtg gctgaagtag tatcagaagc tttaagacct ataagagaaa
     3481 aatatgttga ccttcttaat aataaagatt atttagaaaa aatatatagt atgggagcag
     3541 aaaaagccga aaaacaagct agaaaaactc ttagaaaagt ttataaaaaa gttggattta
     3601 tagagagaag atactaatta ttagctatct ttacttgata aataaatttt aaagttatta
     3661 aaaaggtata tgatatgaaa gagagagtct caaagtgaac ttttgttcat gagacactct
     3721 tttttgtatg aaataaaata tattttaatc acttcaacaa tgaaaaagag gattatctct
     3781 attttgagac agcatctttt gataataaaa aaagatgagc ttttgctcat ctcaaagggg
     3841 ttagattatt cttactttgg ggaaagtaga ataaaggtaa aagttaactg atgtcaactt
     3901 tgtctttttt gcatatcact actgagcata aaaaaatcag aaaaccatag ccttctgaaa
     3961 caacttctaa catacattgt attttatacc caatatgtaa tttatgagtt aattttaact
     4021 cttataatgg aaattgtcaa taatatatca aaaaaatatt ttttaaattt atataattaa
     4081 atgaatttat gtttatatat aggtgatatg atacagata
//
LOCUS       DAMJVX010000242         4043 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.249, whole genome shotgun sequence.
ACCESSION   DAMJVX010000242 DAMJVX010000000
VERSION     DAMJVX010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.249"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(77..553)
                     /locus_tag="PC407_003433"
     CDS             complement(77..553)
                     /locus_tag="PC407_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HDF2498841.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGVLDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQAKDLEEHHHHDKNCGCGCNHHHEN"
     gene            820..1995
                     /locus_tag="PC407_003434"
     CDS             820..1995
                     /locus_tag="PC407_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HDF2498842.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(2076..2879)
                     /gene="proC"
                     /locus_tag="PC407_003435"
     CDS             complement(2076..2879)
                     /gene="proC"
                     /locus_tag="PC407_003435"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2498843.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINNEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(2919..3503)
                     /locus_tag="PC407_003436"
     CDS             complement(2919..3503)
                     /locus_tag="PC407_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2498844.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         1363 a          654 c          496 g         1530 t
ORIGIN      
        1 cttagttttt tgaatgatat atgtctaaaa aaagactgtt tactaaacag tcttttttta
       61 tatatatgct caatatttaa ttttcatgat gatgattaca tccacatcca caatttttat
      121 catggtgatg atgttcttct aaatcttttg cctgttgttc catttcatga aactctttta
      181 ccattatatc ataagttgga agagctgcct cagtatcgta ttcttcccct ctccaatcag
      241 cgtacatcat agcatcccca ttagttaaaa ccaatctacc tgacatagtt atattatcca
      301 cttcttctaa aaattctatt tgtttttgta aaacttctcc ttctaactct acatcttcat
      361 ctaaacattg cactatcatt acaggtgcat aataatcttc ttcatcttta acatttacaa
      421 caatatctaa aactccttct gcaccttctt caaattctgc caactcaaca tttgtatcaa
      481 tcatatcttg agggactaat aaatcttcta ttaaatattt tattactcta tcttttacta
      541 attcttttga catttatttt tctccttccg attctataac attatcataa tcatttctat
      601 attatagtat actacattaa ttgttttttc aatctcttac aaaaattatt tttttattat
      661 cttccttata ttcccttttt tattacaaag ttgtaatcgc attgatttaa aaaatagttt
      721 atatttagta taatagaaat gtagcacata tttttattca atatattaat aaaaaaatag
      781 attgaaaatg taatgtatgt taaatctagg aggaatatta tgaagtttaa aaaacaaatt
      841 agttctattg ttgtgtctac tatgcttgta cttgggagta tgaacctttc ttatgcagat
      901 ggtacaaatg tagttacact tggagctaac ttatcagagt ctcaaaagca gcaaatgctt
      961 aattactttg gagtaaaaaa agaccaagtc ttagttttag atgtaacaaa tgcagaagaa
     1021 agacagtatt tacaaggtgt tgcaacagag ggtcaacttg gaaaagttac tatctcttgc
     1081 tcatacgttg aacctactaa aaaaggaaat ggaattaatg ttaaaacagc aaacttaact
     1141 tgggtaacta gttcaatgat tgcttcaacc cttacaacag ctggtcttga agatgcaaat
     1201 gttattgctg ctgctccata tcctgtaagt ggtactggag cattgacagg aatcatgaaa
     1261 gcatttgagg atgcttcagg aaagaaactt gatgaaacta agaaagaaat tgcttctgaa
     1321 gaattggtag ttacaggtga cttaggagat gatatagggc aagaaaaagc aacaggtgta
     1381 atgaatgata taaaaacaga aattgtaaag aatggtacaa aagataccaa tcaaatagca
     1441 aatacaataa ataatgttgt aaataattac aatattacta ttacaccaga acaaaaagaa
     1501 caaattaaag gtgtaatgaa aaaaatagct gaccaaaatt atgattacaa tagcatgaaa
     1561 aatacactag ataatgtatc agataatgta aatgaacaat taaagaaatt aggtgaaagt
     1621 gttaagggtt ctggtatact agatactatt ggtggatggt ttagtggaat aggagactgg
     1681 ttctctggtt tattctcagg cggagataaa aaagatttgg gaatcttaaa taacactaat
     1741 gatgagtctc ttggctctga tgctgtaata aattctacag aaggtgttca aataaatcca
     1801 caaaataata ctaattctaa caatgatagt gatacaaata gctcaaaaaa tgatacttca
     1861 agtaataatg aaaataacga tagttctcaa acaaatgaaa acaacacaac aaataattca
     1921 gaaaatcaat caaataataa tgactcagtt ggtttctttg aaaaaataaa aaattggctt
     1981 tcaggcttat tttagaaatt aaacaaataa aaagaattgc ctaaaatgga aattttctat
     2041 aaaaggcaat tctctttatt tacaataaat ttttattact cactcatttt tttagatttt
     2101 tctatacatg cacatattgc ttttatcaca gaagctctaa aaccttcttc ttcaagtact
     2161 ttaactgctt ctatagtagt tccaccaggt gaacatacca tatcttttaa ttcaccagga
     2221 tgttttcctg tctcaagtac catttttgca gaacccataa cagcctgtga tgcaaactta
     2281 tatgcttgtg gtcttggcat tcctgcaatt actgctgcat ctgctatagc ttctataaat
     2341 aaaaatacat atgctggtgc agaaccactt gcaccaatta cagcttctat aagatactct
     2401 ggtactacct cagtatttcc aaaagaatta aatacttctg taactgcttc taagtcttca
     2461 ttgtttatat ttttatttat agaaagagaa gacatggctt catttacaag agctggagta
     2521 tttggcatag ttctaactat ttttttatct tctcctataa ttgactctat atcctttata
     2581 gattttcctg ctgctattgt aactataatt ttattgtcat ctataaagtc ttttatctct
     2641 tccaaaatat catcatatat atctggttta acagctacaa ttactatatc agaggttcta
     2701 gttacttcct tcgagtcttt tgtagtattt attccaaaat ttgcatctat tctatcgaga
     2761 gttccttgac tatatgctga tgctataacc atattaggtt caactaattt tgaatttact
     2821 attcccccta tcatagcgct tgccatattt cctgcaccta tgaatccaat ttttttcact
     2881 ttagtacctc cttattgact aatagtaatc gttaaaattt aagcttcttt aactgcatca
     2941 gacttttcaa atggatttgg tatacttact accaaaattt tcactacctt attagtatag
     3001 ttgacccaat tatgattgtt cttttcacta ttatagtgta ttgtgtctcc tggatacatt
     3061 tctatttgtt catctcccaa aaacactgta agagtaccct ctaatacata cacaaattcc
     3121 tcaccttcat gtacgtaaca acaaatttcc tcactactct tacttggaag aatttcaatt
     3181 aatcttggta gcattgtttt ttcctttaaa ctagatgata aatgataatg tataaatgtt
     3241 gagttttcta catcaaatac ttctttttca taacttctta atacagctct tttatgctct
     3301 tttggcttca taaaaaagta agttaattct acatctagta cacttgctat ttttcctaag
     3361 gaatctgttg ccacacttgt taatcctctt tcaagttgtg ataaaaaacc aattgacaag
     3421 tttgtttttt cactcatatc cttcagcgtc atttgctttt gagttctgag ttgctttatt
     3481 tttgctccta tgtccttatt caaaaccatc acccctaaat aattttaatt ttttcataat
     3541 ataaatacaa aaatataaat ataggtactg aacctatctt tatttcattt aaaaaaaatt
     3601 tttataagta ttactataat ttatcaatct ttttttgtct acatttttac gattatttac
     3661 gatttatttt tcacatatgt atatttaatc ttgccaacta aagatacatt ttttataagc
     3721 atttactctt tatataataa aacttaagtt gttcatgtag tcttcttctg gtttaattat
     3781 taaaatttca aattatatta ttatttctac acattattac atcctttagt acaattataa
     3841 cataatttta caatttaatg atatctattc aatgtctttt tttactctac aacatatttt
     3901 attttagagt ttaatactat atatattttt tatatacagc cttgtcattt tttagattgt
     3961 caagagttat tgaagtattt tatgtagatt tatattttat tttctaattt ctcatttaac
     4021 aagaattgct tatgataaaa aat
//
LOCUS       DAMJVX010000243         4034 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.250, whole genome shotgun sequence.
ACCESSION   DAMJVX010000243 DAMJVX010000000
VERSION     DAMJVX010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.250"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(214..597)
                     /locus_tag="PC407_003437"
                     /pseudo
     CDS             complement(214..597)
                     /locus_tag="PC407_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      824..921
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            976..1152
                     /locus_tag="PC407_003438"
     CDS             976..1152
                     /locus_tag="PC407_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498845.1"
                     /translation="MFGNKMDKCTHVLTAYISGSYDYCNFLDTQLDDFILEYGESVVE
                     SCLHQVMVLVSKYN"
     gene            1378..1578
                     /locus_tag="PC407_003439"
     CDS             1378..1578
                     /locus_tag="PC407_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2498846.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(1742..2041)
                     /locus_tag="PC407_003440"
     CDS             complement(1742..2041)
                     /locus_tag="PC407_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498847.1"
                     /translation="MGFYINYVVCKVKILFSSFSKIILFYINYVVCKLFITLKAILLK
                     TQIIVTTHSPHILQIDSKEEMIVLDMAESGNVYKKELELGEYGVLGWTNEGLYLH"
     gene            2263..2445
                     /locus_tag="PC407_003441"
     CDS             2263..2445
                     /locus_tag="PC407_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDF2498848.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            2516..3361
                     /locus_tag="PC407_003442"
     CDS             2516..3361
                     /locus_tag="PC407_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498849.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINNSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(3585..3749)
                     /locus_tag="PC407_003443"
     CDS             complement(3585..3749)
                     /locus_tag="PC407_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498850.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT         1522 a          503 c          471 g         1538 t
ORIGIN      
        1 acagtaattt atctatagtg ttacttatat tttatatata tggctatttt tcaacatttt
       61 acaatatata taacatttat ttttttaact tttgtcagta aaatgtcagc aagacacttt
      121 atccacaatt tttatataag ttttccacaa cttatcccca gtattatcca cataaaaaag
      181 gtagctctta tattattagc taccttttat ttattaatct tcatatttcc agctttttat
      241 ccaacttcct atttctatta ttttttcttc agttatttct tctccatcat gtaattttga
      301 aattaattct gataaaggat tgttatgtaa acctctaaaa actctttttc cctctatttt
      361 gagatattct ctctctttta ttaatactgt atagtgtaca tgcctcccaa ttctctatga
      421 tattaaaaaa ccttttttgg ttaatctagc taaaagtata attgtagttg tatgcttcca
      481 accatatgtt ttttctattt catctgcaac atcttttgat attacagttt tataaccagt
      541 attccatatg catttcataa tttttaattc tgtatctggt aattttttat ttagcataat
      601 atattccttc tttcaattta tgtcacttct tcaaatttat ctagcttaaa ttataatagg
      661 agttttttag aacaaaaact atagttaatt tttaccaggt actctacgtg tttagtctgt
      721 tttttaagtg taaaataatg ttcatatcta taaatttatt taactttcaa acaatcctgt
      781 tgaataataa ctaattctat tgtattattt tagttgtaga ataaaactaa atcggcaaaa
      841 ctagagaaat ttagtgacgc aaagctatag ggactaagac ttatataaat atcttatgag
      901 ttatgtcagc cagttgccaa agagatagtg ttctttttgt ttttatgaaa gttttttagg
      961 gggataataa tttatatgtt tggaaataaa atggataaat gtacacatgt attgactgct
     1021 tatattagcg gttcgtatga ttattgtaat tttttagata cacaattaga tgattttata
     1081 ttagagtacg gagaaagtgt agtagagtct tgtttgcacc aagtgatggt attggtaagt
     1141 aagtataatt agatagacat tgatttctag agaacccttt ttagttttat ctacatttat
     1201 attaatatat agcaatgtaa aacgcttaaa tatattttta atattaagaa taactaaaat
     1261 gttgactaaa aaatataatt aagtagacat ttctattaat ctatgataca atagtatttg
     1321 tggtaataca caattaaatc aagtatatct tgatataggt taataggagt atttttaatg
     1381 aataatggaa tagtgaaatg gtttaataat gaaaaaggat tcggttttat atcaatggaa
     1441 ggtagagatg atgtgtttgc tcatttctca gctatacaaa cttcaggatt taaatcatta
     1501 gaagaaggtc aacaagtaag ctttgatata gttaaaggtg ctagaggtcc tcaagcagaa
     1561 aatataacta tattataata tatagatttt tcgggaaaga ctctttttag agtctttttt
     1621 tattgttagt atatgtaaga taatcattgg tgaagtaaat aaggtatatt gagtaatgtt
     1681 cttgatagtt tatatgctaa tgaatttcaa aaaaataagc actcctataa aaagagtgct
     1741 cttaatgtaa atataagcct tcattggtcc agcctaatac cccgtattct ccaagttcta
     1801 actctttttt atatacatta ccactttcag ccatatctaa cacaatcatt tcttctttag
     1861 aatcaatttg taaaatatga ggactgtgtg ttgtgacaat tatttgtgtt tttaggagaa
     1921 ttgcttttaa tgtaataaaa agtttacata caacatagtt aatataaaac aaaattattt
     1981 ttgagaatga gctaaataaa atcttaactt tacataccac atagttgata taaaacccca
     2041 aaataaactt agcatctcca atgtttacac atatacaatg ctcttaaaat tgcagtgaac
     2101 atgcaatagt gcaattgata atacttatca tatattctca ataccttata tttcaactgc
     2161 taaacctcaa tttgtcacaa atatcgctca ctgcaaaatt tctatatttt tattatatca
     2221 tagcatataa ttaatatttt caattaattt ttacatttaa ttatgtttaa attctataat
     2281 aatgcaactt taatgtttct ttctggaact aatataaaaa ctatttcaga aagattaggt
     2341 catgcagata taaaaataac tatgaatagc tactctcagt ttttagaaga gatggacaga
     2401 gaagcatttg aaaatttgag caaaatacta tttattgtat tttaaagaag atgacttatt
     2461 caatatttga tgctagaata taattaataa aaatttaatt ataggtgatt gtattatgaa
     2521 cattattaaa agcataactt tactcttaac attattaatt gtgatattaa tgacaggatg
     2581 ttccccaaga gaaagtgcta gtgaagttgc aaaaaaatat attgataaga tgaactctgg
     2641 tgactatgaa acagcatata gtttattaag taaagatagt aaaaaaaaaa ttagtataga
     2701 aaatcttgaa gaatttcaaa atattttagt tagtacaaaa agaataatta gttataaaat
     2761 aggtaaagaa aaaatcataa aaaaatataa acatgatgga aaagaatata aaaatgttgt
     2821 taaattagaa gaaactttta atataaaaaa ccttctttat aaaaaagatg attctctaaa
     2881 agtaaataga tattttgtag ttgaaaataa caaatacaag ctattattgt atgaaagttt
     2941 caaaaaagaa tttggtatta atagtatgga ccttgctcaa cttaagctta atgattttgg
     3001 actaaaatac tttaaagaag ttgaccttat actaaaaaaa gctattgcta ttaatcctac
     3061 tgattcaaag ctttattatg cccaagcatg taattatatg aattttgatg gatttcctgg
     3121 ttattcagat acaacatata atgctttaga ttcaattaat aattctttaa agtatttaga
     3181 taaaaatgat tctgactaca aaaaaaatgc aagtaatata tataacttaa aaggagttat
     3241 tcttatgttt cacaaaagat ttgatgaagc acaacaatat ttaaataaag ccttatactt
     3301 taacaaagat aatgaagacc caaaatataa tctaaataca ataaaaaaac atctaaacta
     3361 gatatttaat ttttgaattt ataaaatatt tttttatgta taattcagca ctgaatcata
     3421 atctctacta aactaattac aagtttaaat tataaagact tatttctata taaatattac
     3481 tctataacaa taagtttctt atttaaattt taaaatatta tctaaaaatc ataaaaaaga
     3541 aggtataaag agctaccctc aagctcaata taccttataa atatctaaac ttcatttttt
     3601 actaattttt ctatatcttt gataatatgt tctttagatt tttttccagc tatggcatca
     3661 aaaattgcaa cagccttttt ctctccaaac ttttctgcta catcattaaa gtctaaaaat
     3721 ctaacacttt cttttggttt accaaacata taacacctct catacatttt tatatattta
     3781 ttataacttc tatagactta tttttcacca ataaacgtta gacaaattag tccctttatt
     3841 tttttaaact agctttacct tcaaaattct taacaaaaac agtttattct actctataaa
     3901 taaaaataga caatttaatt tgtaggaaac tgataataga atacagtaaa taaaactttg
     3961 tttaatctat ctgaaaaagt tatctattaa aactgctatg aatttataaa gaatattcta
     4021 gttcataaaa atac
//
LOCUS       DAMJVX010000244         4032 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.251, whole genome shotgun sequence.
ACCESSION   DAMJVX010000244 DAMJVX010000000
VERSION     DAMJVX010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.251"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            203..1015
                     /locus_tag="PC407_003444"
     CDS             203..1015
                     /locus_tag="PC407_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HDF2498851.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            complement(1104..1295)
                     /locus_tag="PC407_003445"
     CDS             complement(1104..1295)
                     /locus_tag="PC407_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498852.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            1600..1806
                     /locus_tag="PC407_003446"
     CDS             1600..1806
                     /locus_tag="PC407_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2498853.1"
                     /translation="MFKKKSGYTFTNFLNKVRVEKNKKYLLKKDLSILDVTVLIGFNS
                     QNYYSIIFKKFNNLTPIEYKNIYN"
     gene            2115..3734
                     /locus_tag="PC407_003447"
     CDS             2115..3734
                     /locus_tag="PC407_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2498854.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGNCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
BASE COUNT         1504 a          482 c          565 g         1481 t
ORIGIN      
        1 ttctataact aaattgaaat aatcactttt tcaagaatgt tataattttt ttattgtatt
       61 ctttttgcta tagtctatga ataaatcaat agaaaatctt ctaagttgct tttaaataat
      121 gactttatgg ttcaattgta ctaaaatagt atatgtaggt tacaaataat ctaataaata
      181 ttaaagtttg gaggtaatag atatggcaga ttgtaattca tgtccatcaa agggaaattg
      241 taatagtcaa tcaaattgtt ctattgaaaa taaccctaac aataaatttg gaaaaataat
      301 tggtgttatg agtgggaagg gtggagttgg aaaatcaact gtaactgctt tacttgctaa
      361 taagctaaac aaaatgggat ataaggttgg tatattagat tcagatataa ctggaccaag
      421 tattcctaga cttatgggtg taaaaaatgt aaaagcttat tctgatggct cttatattta
      481 ccctgtagaa aactcaaata acataaaagt aatgtctata aaccttatga tagatgatga
      541 gaatgaacct gttgtttgga gaggaccttt acttggagga gttgtaaagc aattttatac
      601 tgatgtactt tgggaagaat tagactattt attaatagat atgcctcctg gtactggaga
      661 tgttgcctta acggttatgc aatctattcc tataagtgga atagttatgg tttcagtacc
      721 tcaagatttg gtatctatga ttgtatcaaa agctgttaat atggctaaaa aaatgaacat
      781 aaatgtcctt ggagttattg aaaatatgag ttatatccaa tgtcctgatt gttctaaaaa
      841 aatcaagtta tttgaaggag aatctactga aaagttttta gatgatttag acttagagct
      901 tttaggagaa cttcctatga ctaaagaaat tatagatatt actcataatg gagttacaga
      961 aattagtgat gatttagatt caatattgac taatgttgtt gaaaaaatta aataaataaa
     1021 aattttataa ttttatattt atcaaaaaaa gaggcttgta tttattttat aatcatacaa
     1081 ataaatataa acctctttta tgtttatcta tgttttaaat acacaaaata ttcttctact
     1141 tcatctccat tttttacttt tactaagtat tcattataat gagcattata tggctgtgtc
     1201 attttgcatt ctattacatt ttctatgtct tctaattcta aaagttctac atcctttaaa
     1261 aattttcttt ctatgagttc cattaatctt tccataatat atcctcactt cccctataat
     1321 taagataaac tatatatcct cttaatacag agtttatctg tttattatat tatatccaat
     1381 atattacagt ttctaacatc attcataaat tattttaaca aatagagatt gattttttaa
     1441 taaatatgcc ttgataaaat gaaacataga ccaactctta gttttatgta aagaaaactt
     1501 aaagaaaact atagattata ttcacaaaaa ctactcagaa gatataacag taagtaatat
     1561 atgtaatgaa ttacaactta ataaaagtta tttttttctt tgtttaagaa aaaaagtgga
     1621 tatactttta ctaatttttt aaataaagtt agagttgaaa aaaacaaaaa gtatctatta
     1681 aaaaaagact tgtctatttt agacgttact gtactaattg gattcaatag tcaaaattac
     1741 tatagtataa tttttaaaaa gtttaataac cttacaccaa ttgaatataa aaatatttat
     1801 aactaaaaaa tatcattcta aaaggatgct atttttttac atatttttta caattatagt
     1861 taattctttg ttaaattgat tgtacataaa atactaaaaa tctaagttca agtatttaaa
     1921 ttgctatata tcataaattt tctaaaatta acaacaactt aaaaatttaa cattctctta
     1981 acgtatactt aacaaaacat taatatttct attgacaaaa tttgttattc tatattatgg
     2041 aaacaataaa ttttagttaa taaagtttat aattcagata aaaaaactat taatttctta
     2101 agggaggaaa tatcgtgaat atagtaatta gccttatcgg tggacttgga ctttttcttt
     2161 acggaatgag tttaatggga gaaggtttac aaaaatcagc aggagacaaa ttaaaaaaaa
     2221 ttattgaact cttaacaagt aatgttgtta tgggtgtact ggtaggtaca gtcgttacag
     2281 gtattataca aagttcaagt gctactactg ttatggttgt aggatttgta aatgcaggta
     2341 taatgaatct aagtcaagct attggtgtaa taatgggggc aaatataggt actacagtta
     2401 ctgctcaatt agtttcattt aatcttgaag gaattgcacc aattgcttta ggtataggta
     2461 ttttattcta tttatttaca tcaaatcaaa aaacaaagca tttatctgag atacttatag
     2521 gttttggtat attatttaca ggaatggagt ttatgaaaga tgcagtagct ccacttgctg
     2581 aatataaagc attcactgat gcacttttat attttagtaa aaatcctgta ttaggtatat
     2641 tggcaggatt tgccataact ggtattgttc aaagttcaag tgcatctatg ggtatgttaa
     2701 tagcactagc ttctcaagga atacttcctc tatcatctgc tttacctatt ctttatggag
     2761 ataatatagg tacttgtgtt acttcacttt tatcaagtgt tggtgcaagt agaaatgctc
     2821 gacgtgcagc tgtaatgcat ttaagtttta atgtcattgg tacaataata tttatgcttg
     2881 ttttaaataa acctatatca gccatagtta ctcattttga cccaactgat acagctagac
     2941 aaatagcaaa tgcacatact ttatttaatc ttacaaatgt tataatatta ttaccatttt
     3001 ctaaatacat agttaaatta gcaaatagac ttatacctat taaagaaact gaatcagaaa
     3061 tagtaaataa tactaagtac ttagatgaaa ggatgtttag cactccatct atagcacttg
     3121 gtaatactgt acaagaagtt gtaagaatgg gtcataaagc tacaaactct ttggaacatt
     3181 caattgcagg atttttaaat aagagcaatg aagatattaa taaagctttt gagtcagaaa
     3241 aagttgtcaa taagttacag aaagatatat tgaactattt gttaaaactt tctaaagaac
     3301 ctcttagaga cgatgaacgc tttagaacag atttgctttt taatactgta aatgacattg
     3361 aaagagtctc tgaccatgca gaaaatatag ctgaattggc catgagtgtt aaggaaatga
     3421 acattagttt ttctgatagt gcgataagag aaatatacga aatatacaat aagacaatta
     3481 ctaattttaa ggatgcctta atagttttag atgttaaaga ttttgagtta gcaaataaag
     3541 ttttagaagt tgaaaatgaa gtaaattatt tagaaaaaac ttttagaaat tcacatatga
     3601 ttagactaaa taatggaaat tgtacaattg atgctggtgt actttatctt gacttactta
     3661 ctaatctaga aagaatttct gaccactcaa caaatatagt aaaacaagta ttaaaactta
     3721 aacaaaaaat atagtgatta tttttatata taatcttaat caatgagaag taagaagcta
     3781 tagataatgc ttatatcatt tatggcttct tattttttat gtaatactca aatttttata
     3841 gtattctctt attattaaac agcttctgcc tagaatattc catatatata tgacttcttt
     3901 cataaaactt gaactgccca taactaaagt cacaagtata cataactatt ttttaatcaa
     3961 ttataaccaa tatttatttt atttatcttc attttaattc ttcattcaat aatgtaataa
     4021 gattattttt ta
//
LOCUS       DAMJVX010000245         4004 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.252, whole genome shotgun sequence.
ACCESSION   DAMJVX010000245 DAMJVX010000000
VERSION     DAMJVX010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.252"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            186..1139
                     /locus_tag="PC407_003448"
     CDS             186..1139
                     /locus_tag="PC407_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2498855.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RVPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAVFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            1129..2085
                     /locus_tag="PC407_003449"
     CDS             1129..2085
                     /locus_tag="PC407_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2498856.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKMLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            2082..2837
                     /locus_tag="PC407_003450"
     CDS             2082..2837
                     /locus_tag="PC407_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2498857.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            2929..3894
                     /locus_tag="PC407_003451"
     CDS             2929..3894
                     /locus_tag="PC407_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2498858.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTIKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKTSLPASLEKYKDKKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         1607 a          374 c          676 g         1347 t
ORIGIN      
        1 attggcgttc ataatttatt aaattaaatt ttaatgttac tagaagtttc aattctagta
       61 acatttttta tgttaaaaat agatatagat atatattgac aatgataatt attattgata
      121 aactataaaa gtgaaaatga taataatttt cattttaatt aataaaagat ttttaaggag
      181 ggagaatgaa gaaaaggtat ttagtcatag gaataataat tttgtcttat ttttctttat
      241 ttataggagc tgaagatata aatataatgc atatatttgc aaaaaatcag cataagctta
      301 tgatatttat catgagtagg gtaccaagat taataagtat attgattgct ggtgtaggaa
      361 tgagcatagc aggtcttata atgcaacaga taagtaagaa taaatttgta tcacctacaa
      421 caggtgctac aatagatgca gctcaatttg ggatagtaat atgtatgctt ttagtaccaa
      481 ctgcctctat ttttacaaag acaataatag catttgtatt ttctcttgta gggactttta
      541 cgtttatgaa aataataggg aagttacaat ttaaaaatat aatctttgta ccattagtag
      601 gtataatgtt tggaaatata ataggttcta tgacggattt tatcgcgtat aaatacgatt
      661 tgagtcaaaa tgttagttct tggatgcaag gtgacttttc tatgatactt aaaggaaatt
      721 atgagatatt atatatcaca atacccctta taatattagc atatatttat gcaaataaat
      781 ttactgttgt tggtatgggt atggattttg cgacaaactt aggtcttagt tacaaaagaa
      841 ttgtcaatat aggtctgata atagtagcac ttgttacagt atgtgtagtc gttacagctg
      901 gtaatatccc ttttataggt ctaatagttc ccaatatcgt atccttgtat atgggtgata
      961 atataagagc aagcatatgg tatactggtt tacttggagc ggtatttgta cttatatgtg
     1021 atatttttgg aagaattatt atttatccat atgaaatatc tataggactt acagttggag
     1081 ttataggaag tatactattc ctttatttaa tattaaggag gaatgtgaat gaagcttagt
     1141 ctcaatcaaa aaatttactt tgtaagtata ttactattca tatcaatagc actttttata
     1201 atttatggaa tagacatgaa tcatttagaa tatgcattat cacaaagaat accaaaaata
     1261 tttgctatgg tattaggtgg aggatgtata gcatttacta ctgtagtgtt tcaaacaatt
     1321 acaaacaatc aaatattaac accaagtgta ctaggtttag attcattgta tgtaatgatt
     1381 caaactataa tagtattcat atttggttca agcagtagtc tgattataaa tgaaaattat
     1441 aattttatta ttaatgtagc tatgatgatt ggagcttcat tgttattata taagatgtta
     1501 tttgaaaaaa ataaaaataa tattttcttt ttaatcctag taggtatgat atttggaacc
     1561 ttatttaaaa gtgcaacaac atttattcaa gtaatgatag acccgaatga atttttagct
     1621 ttacaaacta gtataatggc aagtttaaac aatataaaca ctaatgtatt gttaatagca
     1681 tttataataa ttattgccat aatacctttt atatatgatg aaataaagta tttagatgta
     1741 ttgtcgttag gtaaagaaca agcaattaat ttaggtgtag attttgataa ggcagttaaa
     1801 aaaatgataa tattaatagc aattttagtc tcaatatcaa cagcacttat tggacctatg
     1861 acatttttag gacttttact tgctaatata acaagagaaa tttttaaaac atataagcat
     1921 acatacttga tttcgggttc tatgttgata gggatgatta cattgataat aggtcaattt
     1981 tttatacaac atgtatttaa gtttgatacg actttaagtg tagtaataaa ttttatagga
     2041 ggaatttact ttattcaatt actattaaag ggggcaaata gatgatagag ataaaaaata
     2101 tatttaaaag atataagaat aaaaatgttg tagatgatgt ctcttttagt atagaaaagg
     2161 gaaagataac atcttttata ggaccaaatg gagctggaaa aagtacagta ctatcaatag
     2221 ttacaaggct tataggaggt gatggcggag aagtaataat agaaggaaaa agcttgacta
     2281 attatagcaa taaagaatta gcaaaaaaaa tagctattct taaacaatct aacaatataa
     2341 ctttaaagct tacaataaga gaattagttg gatttggaag atttccatat agtgaaggta
     2401 atctaactaa agaggatgaa aattatattg atgaagcaat agaatatatg aagttgactg
     2461 atatacaaca taagtattta gatgaattga gtggaggtca aagacaaaga gcttatatag
     2521 caatggtaat agctcaagat acagagtaca tattacttga tgaaccactg aacaatttgg
     2581 atatgaacca ttccgtacag atgatgaagg tacttagaag tctttgtgat gagttagata
     2641 agactatagt attagtaatg catgatataa attttgcatc atgttattca gataatatag
     2701 ttgcattaaa aaatggaaaa gttgaaaaag taggtcgtac agatgagata gtaaatgaaa
     2761 aagttttaga agatatttat gagatgaatt ttaacattaa gaatataaat ggaaatcgaa
     2821 tctgtattta tttttaaaat attgtatatg aaaataagct tgctatatga ttttttataa
     2881 agtttttaaa ttaaaataaa gattaatagg agagaggaaa atattgatat gaataaaaaa
     2941 gcagcaatag tagcagcagt agctattata ggattagtga cagtgtttgc attaggtgga
     3001 agtaaaaaga atgaatcaaa aacatcagaa aattcaaata ataccataaa gataactcat
     3061 aacttgggag aaacagatgt aaaattaaat cctaaaaaag tagtagtatt tgattattcg
     3121 gctttagata caatggatgc attaggtgta gctgagaatt tagtaggact tccaaaaacg
     3181 agtttaccag catctttaga aaaatataag gataagaagt atgcagattt aggaggttta
     3241 aaagaaccag atttagaagg tataaagtcg gcaaatccag atttaattat aataaatgga
     3301 agacaagaag atttttatga acaattgtca aaaatagcac caacaataag tacaagtaaa
     3361 gatgataaga agtacttaga atctgttaaa aataatatag acaaaatagc aaaaatattt
     3421 ggagtagaag aaaaagcaaa tcaagaattt agtaaaattg aaaagaaaat agaaactttg
     3481 aataaaaaag taacagataa aaatttaaat gctttaacta taatggtaaa tgaaggtaat
     3541 ttaagtgtat ttggtgaaga atcaagattt agtatattat acaatagttt tggatttgaa
     3601 aataaagata aaaatataaa agaatcaagt catggacaaa atattacttt tgaatatata
     3661 gctaaacaaa atccagaagt tatgttcgtt atagatagag gaatagctac tggtagtgat
     3721 gtaaaagaaa gctcaactgc aaaatctgtt ttgaacaatg atataataaa atctatggat
     3781 gcatataaga atgataatat aatatattta gattcaccaa catggtatgt aaatgatggt
     3841 ggtctaacat ctttaaataa aatgatagat gatgcttcaa aagcggtaaa ttaataatcc
     3901 gatataatca aaatgtattt tttgtaaaat gattatcaaa taaactagga gtttttaaga
     3961 actcctagtt tattttttgc ataataaaga tatagtttaa acaa
//
LOCUS       DAMJVX010000246         3969 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.253, whole genome shotgun sequence.
ACCESSION   DAMJVX010000246 DAMJVX010000000
VERSION     DAMJVX010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.253"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            1..391
                     /locus_tag="PC407_003452"
                     /pseudo
     CDS             1..391
                     /locus_tag="PC407_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            complement(751..1905)
                     /gene="nagA"
                     /locus_tag="PC407_003453"
     CDS             complement(751..1905)
                     /gene="nagA"
                     /locus_tag="PC407_003453"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2498859.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNITDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQRKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFSVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNIATLEDAIKM
                     ATYVPALSCNIDNVCGSIHQGMDADFIVTDKNFNIYETYISGICMYKVNK"
     gene            complement(1988..2713)
                     /locus_tag="PC407_003454"
     CDS             complement(1988..2713)
                     /locus_tag="PC407_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2498860.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRIQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            2982..3911
                     /gene="pfkB"
                     /locus_tag="PC407_003455"
     CDS             2982..3911
                     /gene="pfkB"
                     /locus_tag="PC407_003455"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2498861.1"
                     /translation="MITVITFNPSIDRMYKVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEYNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
BASE COUNT         1446 a          550 c          562 g         1411 t
ORIGIN      
        1 tgcaaaagat taaaagaaag tatatgctgc aattacagag gaagtagcat taagtgagct
       61 atcgctctta gaggaaaaat ggggtaatca atatgcgata gcattaagaa gttggcgaag
      121 caattgaaat gagctagcaa catactttaa atatcctcaa gagataagaa cattgatata
      181 tactacaaat tcaatggaaa gttacaatag attattaaga aaagttacta aatcgaagtc
      241 tatttttcct ttagatgatt ctttgcacaa atcattatac ttagcaacta tggatatttc
      301 tgaaagatgg actcaaaaga ttagaaattg gactcaaatc ttagctcatt taagtattta
      361 ttttgaagaa agaattttaa ctctaaatta ataaagaaat atttcaaaaa aattaaggaa
      421 ttttcttaaa ggggtaataa tttttacaaa aatggaaaaa atatcaaaat aaggctatga
      481 tttttaaagc catagcctta ccaataaatg tttaattatc actaaaaaat aaatttttat
      541 tcaaagaata cacataaata tttccagatt cttaatttta taaacttata ttgtaaaata
      601 aagaacctta ataaaaggag tgtctcaaaa ttaacttttt agtttatgag gcactctttt
      661 tgtatgaaat caaatgtatt ttcatcattt taacaatgaa aaagagactt atctctattt
      721 ttaggaagcc tccataactt tatattgaat ctatttatta actttgtaca tacatatacc
      781 acttatataa gtttcgtata tattaaagtt cttatctgtt actataaagt ctgcatccat
      841 accttgatgt atggagccac agacattatc aatattacaa cttaaagcag gaacatatgt
      901 agccattttt attgcatctt ctaaagtggc tatgttccaa tcaactatat ttctcacagc
      961 cttgtccata gttaatacac ttccagctaa actcccacta tttaatcttg caacactatt
     1021 ttttacattt acactaaact taccaagagt atattttcca tcctccatag caccagccct
     1081 catacaatct gtaattaaag ctatgttatt actagacttc gcatccatca ttattttaac
     1141 agctattggg cttacatgat gaccatcaca aattaattca gcataagtat cttttagtac
     1201 catagcagcc ccaaccattc caggttctct atgatttaaa gggctcatcc cattatatgt
     1261 atgtacaaac actttagcac ctgaattaac gacttgttta acttgttcaa atgtagctga
     1321 actatggcca agagaaacag agatattatt tttagttgca tatcttgtta attctattga
     1381 acctttacgt tctggagcaa ttgctatttt tcttattaaa tttttcgatt ttctttgcca
     1441 taattttaat atattgatgt cagggtcttt aaaatatttt tcattttgag cgcccttata
     1501 tttttctgta aaaaatggac cttctaaata aatcccttgt atttttgcac cctcaactcg
     1561 attgaagcta ttgcttatat tttctacaga atcacttagt gtatctacag aatcagttaa
     1621 agttgtaggt aggaaagatg ttactccata ttttaataag ccttttgaaa ttgtgtttat
     1681 tgcttcaaat gtattgtcca tgacatctgc acctaaaaga ccgtgaatgt gagtatcaac
     1741 taagccagga gcaatatatt tacctgaaaa atctatgaca ttatcagtaa tattaggtct
     1801 ttcttgttga aattcaccga atttgccatt aattatacta aggtaacctt tttctttgat
     1861 tccatgtttc ataaaaaatt tatctgcata aataaaactt ttcaaaataa cctccttata
     1921 attatactat cattgtggca ttgacgttta taatatcatt ctattttctt gaaagctatt
     1981 taattcacta tcttacatgt cttactctat atacaaattt atcgcttcta gcaacactca
     2041 aagtaaattc aataactata ttgtcatcat tatatgtggt acgaagtaat ttcagacaag
     2101 gtgaatctcc tggtacttcc aataacttag cttccttatc agagattatt ccagctgaaa
     2161 attcttcatc agcaacttta ataatctcgc cataatcttc tctaaatatt tcatataaag
     2221 gtttttgttc gattttttct tttgtcaatc ctataaattc tttcatagga agatatgttc
     2281 tttcaagcat cataggaagg tcatcagcaa gtcttaaacg cattattttt atcatttttt
     2341 caccaggagt tatacctaat ttttcagaga aatactttgt actttctact acctcaaatg
     2401 ataatacctt tgtacctggc ttttttcctt gtattctcat ataatctgta aagctataac
     2461 tttccattaa gttttgagaa ttttgactta gtgcagcaac aaaagttcct tttccatgtc
     2521 ttttgtatat atatcccata tgttccaact cagccatagc tagtctaact gtggttctac
     2581 ttactccata ttcatcacaa atttttcttt cagaaagcat tctatcgttt ggttccatct
     2641 cattttcaat cttagttctt aatatctcaa caagctggtc atatattggt cttttatcat
     2701 ttctttttat cataaaaaca tagtcctttc taagtggtta ttaccacaat ttgattatag
     2761 aatatagaat tatttatgtc aatgaataaa gtttaatcaa aattcaataa gtaaggctat
     2821 taatttatta taaaattatg gtatattgac aaaacaagta aaataattta tattcaaatt
     2881 ggtaataacc actttgggta tattatagat gaggtatttt ttattttaca gattaattta
     2941 ttgtttagaa aagtaaaaat taataatgag gagaatctag tatgattaca gtaataacat
     3001 tcaacccatc tatagataga atgtataaag tgaataatat aaacataggt gaggtgcaaa
     3061 gggtagtaag taagaatgca actgctggag gtaaaggatt aaacgtagct aaggtatgta
     3121 aaattttaaa agaaaatcct ttagctatgg gatttctagg tggatttaat ggagaattta
     3181 taaaaaatga acttagaaaa ctagatatac aaaataaatt tacaaaaata gagcaggaaa
     3241 ctagaaattg cttaaacata attgaggaca ataaagttag tacagaattt ttagaaaaag
     3301 gaccaatagt agaaaaaagc gatttagaga gatttgaaaa tgatatagag gaagtactaa
     3361 aaaatactaa aatactagta gcttctggta gttattgtga gaatatgccg atagattatt
     3421 atgaaaagat aggaaatata tgtagagcaa ataatataaa atttatatta gatacatcag
     3481 gagaagcatt aaaaatagct ttaaaatcaa aaccatattt aataaagcca aatatagatg
     3541 aaataagaca tttattagga ataaatatag aatcaagaga ggaaattatc ttgtcaggaa
     3601 aaaaacttat tgaaatgggt gcagagaatg tatgtatatc tttaggaaaa gatggtatgg
     3661 tgtatttaaa cgaatataat gtatatgatg taaaagtacc agttgtaaag tgtataaata
     3721 ctgtaggtag tggagatagc acagtggctg gatttagtgt aggtatttta aggggttata
     3781 aaatagaaaa tttacttaaa tttgcaaatg cttgcggtat aagtaatgct ttaaatatgg
     3841 aaactggatt tgtagatttg ggagaaattg ataaatatca agatttagtt gaagtaaaaa
     3901 agttaagtta aataaattta atgggaatat aataaattta atgttgttgt aatagatttt
     3961 aattttaat
//
LOCUS       DAMJVX010000247         3939 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.254, whole genome shotgun sequence.
ACCESSION   DAMJVX010000247 DAMJVX010000000
VERSION     DAMJVX010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.254"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..239
                     /locus_tag="PC407_003456"
     CDS             <1..239
                     /locus_tag="PC407_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005209661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498862.1"
                     /translation="VEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRTAIEF
                     STEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            497..1546
                     /gene="carA"
                     /locus_tag="PC407_003457"
     CDS             497..1546
                     /gene="carA"
                     /locus_tag="PC407_003457"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2498863.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            1739..>3939
                     /gene="carB"
                     /locus_tag="PC407_003458"
     CDS             1739..>3939
                     /gene="carB"
                     /locus_tag="PC407_003458"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HDF2498864.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYL"
BASE COUNT         1630 a          400 c          777 g         1132 t
ORIGIN      
        1 gagtagaaga atctagacca aacatattag atgctataag aaataaacaa gttgatatag
       61 taataaatac accaactaaa ggaaatgact ctacaagaga tggatttaag ataagaagaa
      121 cagctattga attttctact gaaataatga catctttgga tacactaaaa gctttagtag
      181 aagttaagaa gaaacattta aataaagatg agttaaaagt ttacaatata gctgaataga
      241 aattaagttg attgtatagt ttaagaattt aattattaaa taaaaaaaaa gaggttattt
      301 tttctaggag tttttacttt tgagataact tctttttttt aagaaatttg tagtataata
      361 gaaaaagata aaaaataaat gtaaataagt ataaaactag aatgtaaaag tgaaaaatag
      421 atatagatat ttaaaagctt atatttagtt aatgttcttt agtttgtcta aagagaatta
      481 taggaggtac tgtagtatga aagcaaggtt aatattagaa gatggaacag tttttatagg
      541 aaaggcattt ggatatttag aagaaagtgt aggagaagta gtatttaata cttcgatgat
      601 aggatatggt gaagttttaa cagacccttc ttactatggt caaatagtta ctatgactta
      661 tcctcttgta ggaaattatg gtataaattt atcatcagca gagtctgaaa aagttcaagt
      721 aaagggattc atagtaaggg agaaaagtga ttctccaagt aattttagat gtgaaattga
      781 tatagaccaa tatttaaagc aaaataaagt tatcggactt gaaggaatag atacaagagc
      841 tttgactaag atacttagaa ataatggaac aatgaaaggt ataattacat tagaagacag
      901 taaattagaa gatgttaaac ataaattgga taagttttca aatacagaag cagttagaac
      961 tgttacaaga aaagaagtag agcatataaa aggaaatggt ccaaaagtgg ctgttatgga
     1021 ttttggagta aaaagaaata tattaagatc atttatagct cgtggatgtg atataacaat
     1081 atttccagct acaacatctc cagaagatgt attgagtata aaccctgact taatattctt
     1141 gtcaaatgga cctggagacc cagaagacct agaagatgtt atagaaaata taaaagctct
     1201 tattggtaaa aaacctatag ttggaatatg cttaggacat caacttttag ctttagctct
     1261 tggaggaaaa actgctaaac ttaaatttgg gcatagaggt ggaaatcatc cagttaaaga
     1321 tttagaagaa ggaaaagtat ttataacttc tcaaaatcat ggttattatg tttcagaagt
     1381 tcctgaacaa atgaaagtga cacatataaa tttaaatgat aatactgttg aaggaatgag
     1441 acatgagaag ctagatgtat acagtgttca ataccatcct gaagcttgcc caggaccaaa
     1501 agataatgac tatatttttg ataaattttt agaattagta aaataaataa cattagaata
     1561 gctaactaaa aaacaagaat aacttaaatc aaaaagtttg gtttaattaa catagatgta
     1621 ttaaatagat gtattgatga gtaaatttga acttaagata aaataaagta attaaagttg
     1681 attgtataga tgatgaagat aagaactgtg aaaattgttt agataaggag aaagagttat
     1741 gcctaaatta gatagtataa agaaaacttt agtattaggg tcagggccaa taataatagg
     1801 tcaagctgca gagtttgact attcaggaac acaggcctgc caagcattaa aagaagaggg
     1861 aattgaagtt gtattagtaa atagtaaccc agctactata atgactgata aagaaattgc
     1921 agataaaata tatatagaac cattaacaat agaatttata gaaaaaataa tagaaaaaga
     1981 aagaccagac agtttacttg caggtatggg tggtcagaca ggattaaact tagctgtaga
     2041 attacatgat gctggtatat tagataaata taatgtaaaa gttataggaa catctataga
     2101 atctataaaa aagggcgaag atagagattt atttagagaa gtaatgaaag aaataaatca
     2161 accagtaata gttagtgata tagttacaaa tttagaagca ggtcttgagt ttgccaataa
     2221 gataggatat ccagtagttg taagaccagc atatacgcta ggtggaactg gtggaggtat
     2281 agctgatact gaggaagaat taagagaaat actttcacat ggactacagc taagtcctgt
     2341 tggacaagtt cttcttgaaa agagtataaa aggttggaaa gaaatagaat atgaagtaat
     2401 gagagatgga aatggaaact gtataactgt atgtaatatg gaaaatgtag acccagttgg
     2461 agttcataca ggagacagta tagttgttgc tccaagtcaa actctaagtg atgaagagta
     2521 tcaactactt agaaaggcat caatagacat aataaatgca atagaggttc aaggtggatg
     2581 taacgttcaa atagctttaa acccacatag tttagaatat gcgataatag aaataaaccc
     2641 aagagttagt aggtcatcag ccctagcatc aaaagctaca ggatacccaa tagcaaaagt
     2701 agcagctaag atagctttag gttatacttt ggatgagata gaaaatgcag taactaaaaa
     2761 aacttatgct tgttttgagc caacattaga ttatgtggta gtaaaaatac caaagtggcc
     2821 atttgataaa tttagacatg caaatagaaa attaggaact aagatgatgg caactggtga
     2881 aatcatgagt ataggaagta actttgaggc tgctattctt aaagggataa gatcacttga
     2941 aacaggaaaa tattcattgg ttcatgctcc atcagaaggt agaacattag aagaattaaa
     3001 ggctagagta gtagtacctg atgatgagag actatttgac ttagctgaaa tgataagaag
     3061 aggctataaa gtagaaatga tatcagaaat aactggtgtt gataagtggt ttataaataa
     3121 atttaaatgg atagtagaac aagaagaaaa gttaaaagtt ctaaaaatag aagacttaga
     3181 taaagaatat ttacatgaat taaagaaaaa gggattttct gataaaggta tatctgactt
     3241 aatgaagata agcccagaaa aattatatga attgagaagt ttatataata tacaaccagt
     3301 ttataaaatg gttgatacat gtggaggaga atttgaagct ctatcacctt attactattc
     3361 aacttatgaa caatatgatg aggtagtagt tagtgataag agaaaagttg tagtattggg
     3421 ttctggtcca ataagaatag gtcaaggtat agaatttgac tattgttcag ttcactgtgt
     3481 aaaatcttta agaaaaatgg gaatagaaac tataattgta aacaataacc ctgaaactgt
     3541 aagtacggat tttgatacat ctgataagtt gtattttgaa ccattaacag aagaagaagt
     3601 tttaaatata atagaaaaag aaaaaccaga aggtgtaata ctacagtttg gtggacaaac
     3661 agctataaaa ttggctaagt ttttacatga aaaaaatata cctatactgg gaacagactt
     3721 tagagatata gatgcagcag aagatagaga aaaatttgat gatttactag agagactaga
     3781 tataaataga ccaaaaggaa aaggtgtatg gacaactaat gaaggtgttg aaattgctaa
     3841 ggagttaggt tatccagtat tagttagacc atcttatgta cttggtggtc aaggtatgga
     3901 aataacttat aacgaagaaa aactaattca gtatttgga
//
LOCUS       DAMJVX010000248         3919 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.255, whole genome shotgun sequence.
ACCESSION   DAMJVX010000248 DAMJVX010000000
VERSION     DAMJVX010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.255"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            135..1181
                     /locus_tag="PC407_003459"
     CDS             135..1181
                     /locus_tag="PC407_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HDF2498865.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DGGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDERIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPSKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            1289..2452
                     /locus_tag="PC407_003460"
     CDS             1289..2452
                     /locus_tag="PC407_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2498866.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKIIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAQAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SKNPSSTDLQEIAKEITDISKKDYEIFSMYTGDDNWYYMIQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(2541..2921)
                     /locus_tag="PC407_003461"
     CDS             complement(2541..2921)
                     /locus_tag="PC407_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HDF2498867.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(2924..3583)
                     /locus_tag="PC407_003462"
     CDS             complement(2924..3583)
                     /locus_tag="PC407_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDF2498868.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
BASE COUNT         1510 a          434 c          589 g         1386 t
ORIGIN      
        1 ttccaaatga cttaataatg tgaaaatata agacctaaaa tataaatttt ataaatacaa
       61 tagttattga ctctctcatt gtaagagggt ttatactgaa tctaatgaaa atttatagtg
      121 aggagataga ttatatgaga aaaataattg aaattaaaca tcattcacat gattatgagt
      181 gtatgtggaa tggtatagaa gatttatata taaataaaac cggtgaatgc cttccaaacg
      241 attttttctt tttactgtca ggttttggtt cattttgtta tatgaaaaca aataaagctg
      301 atttgaaaag aatggtagct ttgggagatg gaaggacaaa acagatgtat aaatttttag
      361 caccaattgt tggatttgaa tacaaacatc atgagtttaa aaagtatgag caagcattaa
      421 agaaagcaaa atctgaaatt gatggtggat atccagttat tttaggagct ttagatatgt
      481 attatctacc atattataag aagctatatc ataaagaaca cattcctttt cattatatat
      541 taatggtagg atatgatgat gaaagaatat atctgtatga ttgtggtaga acagaattat
      601 tacatctttc ttatgataaa ttacgtgaaa gcatgaattg caattcccct ggattaagca
      661 aagaaaatac tatttgcaca ataagaatga atacttcaaa gaataaatat aacattgcta
      721 gagaagctat tgctataaag cgtgatatgt ttttaaatcc atctaaagga tttttgggat
      781 ataaaggttt tgaaaagttt attaaagagt tgccacagtg gaacactgaa ttggataaag
      841 atgattatga taagattttg accaatatgg ttatgttttt tggaacagta ccaacaattc
      901 caaatgcaat caaaggaatt aaagaaccag atactgttca atttaaaggt ggatttgata
      961 aaatgagtag aatgctcaac agtattgggg aggaatacaa aaacgactct tttatacaag
     1021 tagctgatat ttttgataaa actgctatta taattgagaa aatatccaat attataatag
     1081 attatttaac tcaaacatgt gatgatacag aacaacttcc gttattgttc tctgaggtct
     1141 tagagcatat gaaaacagga tatttaatct tggatgtata aaaaatatat tgttgaccct
     1201 aacattacgt aatagtgtaa tatgaaaata tagcttgaat atattagtag gtattgatgg
     1261 ctgtaaaatt aaaatttgag gtgtggatat gagaacagtg aaacaagttt ctgatttaac
     1321 tggaataagt gtgagggcat tacattatta tgatgagata ggtcttttaa aaccaaataa
     1381 aattacagat gcaggatata gactttatga tgatgaatct attaaaactc ttcaacaaat
     1441 tttatttttt aaggagattg atattccttt aagagaggtt aaggaaataa tgtcaagcca
     1501 atattttgat aaggttgaag ctctaaaaaa tcaaaaaaaa ttacttattt taaaacgaaa
     1561 gagactggat gaattgatag agcttataaa tcaaactcta agaggagaag gaaatattga
     1621 ttttaaagag tttgatatga gcgagtattt taatgtttta gaagaattta aaagagaaca
     1681 taggaataag ataattaaaa tttatggtag tgtagaaaaa tacaatgaat atatagaaag
     1741 agttaagtct aatgaagaga aaattgctaa aatggctata aaacaatatg gaactattga
     1801 gaagtttgcc caagctataa aaacaaattt tagcagtgat atattgaatt taggagaaaa
     1861 atttgataga tataaaaatg attgtttaaa agaaaaacat ccaaaattaa aagaattata
     1921 tagaaagctt gtagaagatt taagtaagaa tccttcttca acagaccttc aagaaatagc
     1981 aaaagagata acagatatat ctaaaaaaga ttatgaaatt ttctctatgt atactggtga
     2041 tgacaactgg tattatatga ttcaaaatta tctagtaaat cctatgtgga tagaagaagt
     2101 agataaaaag tatggaagtg gagcaggcaa gtttattgga caagtactaa agacttatct
     2161 aagagataga aaaccaaaga taaatacatt gtatgaaaaa cttgtagaag atttgagtag
     2221 agattgttct tcaagggaag ttcaaagcat tgttgaagaa atagacaatg aaatgaaaag
     2281 aagtaatgaa ttttataaaa tagacaatgg tgaaagatac tttgattata tgtcagagct
     2341 ttatttacaa gattctaact atataaaagt aactgataag aattatggtg atggtgcatc
     2401 taagtttatt ggagaagctt ttaagattta ttttgataat aataattgtt gaaaatttgg
     2461 aagtaaaaaa tatgtaagtt ttataaaagc acaaaaataa ggatgtagat tagctccatc
     2521 tatatcctta tttaaagtta ttaaaatata tagtttacat caaacttaac actttcaata
     2581 atatttccca aatatgtttt tatactttca acttcttcta aagattttaa tttattttca
     2641 gtataaattg ctttgtccag aatattaaac cttaatccta tttcatcaaa gtactcatga
     2701 tttaaaactt tgtaccaatg gtttttttct ttattccaac tcgtagatat tgaatcagct
     2761 tctttttgag ctttttccca atcttcattt tctatagatt tttctaaaac agatatattg
     2821 tctttatatc cttctgtaaa atcatatagt ttattgttaa cgtaaaatcc aaataataca
     2881 aataagatag tccatagtat aacaaatgtt gtagatttca atattaatca ccctttttat
     2941 tttgaataaa tattttgtca ttttcatcta aaacacaaat aagaacatct ttaaaagttt
     3001 ctatgtgatt agattttaaa atacccatta accagttagt atctttttga gctatgtcta
     3061 gatttttatt tattattcta ccatccagta taagtgggat aggtaaatga ttaaaagctc
     3121 ttggaggagt actattgtag cttgatgctg gtacaacact tagattacca tcagtctcca
     3181 aaatagcata ttgtacatct tttaggttga agtatccttg aatcctaagt tgttctaata
     3241 attcatctat actaattctt tcttttttaa gctctttata tacaattttt cctttatcta
     3301 ttaatacaga aggtttacct gataaaaatg aacttaattt tctgcttttt aatgataaga
     3361 atgaaataag agtctgcatg attacaagac cagatattgc tgcaacacca tttatgattg
     3421 gtatattatt attttccatt gggacagatg caacttctgc aattaatagt gttataacta
     3481 aatcaaaaca gttcatttca gctatttcac cttttcccat gactctaagt gcaattagca
     3541 ctgttatgta taatataata cttcttatta atactactgt catatctatc aattactcct
     3601 ttaaaaaata tttacactta gttagtatat cctatataaa aaatttaatt caaagtataa
     3661 aaaaccttaa ataattggat tcaactattt aaggtttttt tgataatttt aatattaaat
     3721 tttttatgga ttttctgtta cttttattta ttttaataac agattcaaca aaactctttt
     3781 tatctcctaa ttttaagata aaatcaggat attcttcaaa ttcattgagt tttattaatt
     3841 tgaaagaaga agatagattt ttaaagttaa tgattttaga gttattcact aatacacttc
     3901 tttttttaaa ataaatttc
//
LOCUS       DAMJVX010000249         3901 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.256, whole genome shotgun sequence.
ACCESSION   DAMJVX010000249 DAMJVX010000000
VERSION     DAMJVX010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.256"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            182..856
                     /gene="fsa"
                     /locus_tag="PC407_003463"
     CDS             182..856
                     /gene="fsa"
                     /locus_tag="PC407_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2498869.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILDEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(978..2372)
                     /locus_tag="PC407_003464"
     CDS             complement(978..2372)
                     /locus_tag="PC407_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDF2498870.1"
                     /translation="MGIILKPKQFYMTVIVTIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(2450..3796)
                     /locus_tag="PC407_003465"
     CDS             complement(2450..3796)
                     /locus_tag="PC407_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2498871.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGIQQMLFSIGNMTLQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGYWKTKAIVNHRINE"
BASE COUNT         1556 a          538 c          534 g         1273 t
ORIGIN      
        1 ttaatataaa aagtatgttt tttctaacta tatatttaga atttagtaaa taaaaaaatt
       61 ataaaactaa gaaggaaaag caaaacaata ataaaaaata aaaaacaata agcaaaatgt
      121 tgaaaataat accaaatcaa tatataataa gaataaagaa agattaaagg gggatatttt
      181 aatgcaacta atcttagata caggtaatgt ggaggagatt aaggagttat gtacttgttt
      241 accaatagat ggtgtaacta caaatccaag tattgtttca aaagaaaaaa agaattttaa
      301 gcaacttata aatgaaattg gtgagattat aggtgaggat atgcctatac atgctcaagt
      361 gttaagtaca aagtatgaag aaatattaga cgaagcatta tatataagta gtttgagaaa
      421 aaatatatat gtaaaaatac ctgtaacaca agatggttta agagctataa aagaccttca
      481 cagaaaaggt gtaaaaataa ctgctactgc aatattcact gctcatcaag gttttttagc
      541 tgcaaaggct ggagcaaatt atattgcacc ttatgtaaat aggcttgata atatatctgg
      601 tgatggagta gctatggttt cagaacttat aaaaataata gatacttata aaatggatac
      661 aaaagtactt gcagccagtt ttaaaaatgc acaacaagtt atagaattaa tgcaacatgg
      721 ggtgcatagt gttacagtac catatgatat ttgtaaatct atgatgaatc atccattaac
      781 tgattggagt gtagataagt ttatagagga ttgggagaat acttttggaa aaggaagtaa
      841 aacaaataat atttagtatt atggtaaaag agtagttttg tccaatttca caagaactta
      901 tataaaaaga tgaaaatatg aattttcttt ttaataattt cgttattatc ttaagttaat
      961 tcatattttc atctttttta gatttctagt cctataaatt tccaccaagg gaatgttata
     1021 catattatta taaagtaggt taaaggaatc aaagttaaac cgtatttgaa aataaccttg
     1081 ctattataca gattttcacc atgaccaacc attattgtag catgatgaaa tggtaatatg
     1141 tactgtatgc ttactataat gtagacaaat agacataaag taacactatt ttcctttagt
     1201 atatgtaaac ttccaagtaa tggtattact acagatgatg tagtgacagc acttccaaga
     1261 catatattca agattattgt gagagttatc aaaaacaata ttgaaataat ttctgaagag
     1321 tttggtataa actttatcaa ataatttccc attatatcta caactccaga gtactttaac
     1381 actcctccaa ttgaaaatgc tgctataaaa aaaataagga ccttccaatt tatttctttt
     1441 aaagtactaa atccaactat ttttcttagt aataaaagaa gtacacaaat tgacattata
     1501 attaatgtgt ttatgttatg tatgaactga gttaaaaaca taaaaaacat tattcctagg
     1561 cataaaaata cgtgtttttg ctctttgttt agcaaattaa attctttttt taaattagaa
     1621 tatgaaaaat gaagtttatt accctcaaga ttttcattag aaataaattt tattgaatgc
     1681 aattcttttt taaagacaaa gataaataaa aaataagtta taaaacaagt aattatagtt
     1741 ggaacagaca tataaaaagc ccattgaccc caatttatat tacaatttcc aagttctagt
     1801 acaacataat ttagaagggt gtctccattt acaaaaaaca ttgatgtaaa ggtagatgct
     1861 gtaaatatac taaataaaag tatagatttt gaatcttcag ttatctgttg ttcttttaaa
     1921 aactccttgt aaaaagcaga tacaagaata acccttggaa atggttgtgg gataaaaaat
     1981 attaaaagaa tacctaaaat atacgataaa agaatcatct ttttaggggt atttacagta
     2041 tttaaaatta acttctcaga aataattttt gcgacacctg tttttgtaac tgcattagta
     2101 attatgtatg caagaattat tatgtaaaaa ttttctgtaa ataaaaactg taaaataacc
     2161 tttattggtg caatgttaaa tataaacgct agaattacaa aaaacaatgc aacaaaagtt
     2221 ttttcaactg agtctacagc ccatatcaag atagttaaaa aagataagca tatagaaaat
     2281 tttatgttga aactgtaatt aagagagaag tagagaatta tggtaactat aacagtcata
     2341 taaaattgtt ttggcttaag aataataccc atttttatat tcacctccat aatttattat
     2401 atcataatgg aggtgaatac aatgaatgtg aatacatttt ataaaaagat tattcattga
     2461 ttctatgatt tacaatagct ttagttttcc aatacccttt cttatagtag ataactgtaa
     2521 caataagacc taaagtccaa ccaaaaggaa tacccatcca aattccgtct gttccaaaaa
     2581 atttagaaag catataagaa gctggaagtc ttccaagcca taaagatact attgagctta
     2641 taagtgcaaa catggaatca ccagcacctt tcattgcact tgaaagtaca aaagaagttc
     2701 caataaataa aaagaatggt cctattataa ataagtaaga agagccaatt ttaatgacat
     2761 ctttatcagt attgaataga gcaattagat tttccctaaa tagcaaaatt aaagcaataa
     2821 ctgtaactgc taaaactaag gtcattttta tagattctcg aatgcctttt ttaacccttt
     2881 cattttctcc agcacctatg ttttgagcaa caaaagtaga aacagcagaa ccaagattca
     2941 taataggtaa gcttataaaa ttttctatcc taagaccagc accaaaagct gccatagcag
     3001 atgtaccata actattaact aaaaattgaa gagtcatatt tccaattgaa aacaacattt
     3061 gttgaattcc acttggaagt cctaatctaa gtccatcttt aaatattttg ttatcgtata
     3121 caattccttt gattttaaat ttaagcactt catgtttttt atttaaataa attatactta
     3181 ttataaatga tactgcctga gccattatag tggcaagtgc aacgccctca acacccattc
     3241 ttagcactac aataaaagtt aagtctaaaa ctatatttag aattgtggct attatcagaa
     3301 aataaagtgg agttactgaa tctccaagtc ctctaagtat tgcacttact gaattatatc
     3361 cagcagaaaa taatattcca ctaaatatta tagtaagata caattttgat tgagcaaaaa
     3421 cggattctgg tgtattcata agcttaagca ttgggccacc aaatactatt ccaagaatac
     3481 ttattaatat agaaccaata aataaaagta tatatgtagt atcaatagta gcttttacct
     3541 tttttaaatc tcctgagcca taaaattgtg acactaaaat agaaaatcca aggctaacac
     3601 ccataagcgc tgccaccaat aaaaacatta tagggttagc tccagagact gccgccattg
     3661 cttcttttcc gataaatcta ccaactatta tactgtcagc tgtattgtat agttgttgaa
     3721 ataaactacc aataagcata ggcatggcaa aaaagaatat ggattttccc tcatgacctg
     3781 tagttaaatc tttcacctta ttcctccaaa taaaaaaatt attaatttat tatagtataa
     3841 tcttaacata aactttatat ccaaactata aataaattgt atcaaaatat catatatttt
     3901 t
//
LOCUS       DAMJVX010000250         3869 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.257, whole genome shotgun sequence.
ACCESSION   DAMJVX010000250 DAMJVX010000000
VERSION     DAMJVX010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.257"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..548
                     /locus_tag="PC407_003466"
     CDS             <1..548
                     /locus_tag="PC407_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HDF2498872.1"
                     /translation="SHINILIIADINNKDICLKLNIKYMFNLININRQLYPAENSKNN
                     DKKEGMKNNIDSSILLADDLLSIYRLLKKIKIHELYSNINFGTGNIGLTSSVYVLINT
                     LYGNLFNMIDAEKMYLNVNPDFTKDYVLGNIRIHIRPRIKALFNIIIMINKIMNKNKG
                     NKEGDSNESNRFDTKSYGNNS"
     gene            502..930
                     /gene="ytfJ"
                     /locus_tag="PC407_003467"
     CDS             502..930
                     /gene="ytfJ"
                     /locus_tag="PC407_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HDF2498873.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1178..1618
                     /locus_tag="PC407_003468"
     CDS             1178..1618
                     /locus_tag="PC407_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2498874.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            1634..3085
                     /locus_tag="PC407_003469"
     CDS             1634..3085
                     /locus_tag="PC407_003469"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDF2498875.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGSFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAIETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3176..3844)
                     /locus_tag="PC407_003470"
     CDS             complement(3176..3844)
                     /locus_tag="PC407_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HDF2498876.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSQYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT         1621 a          360 c          659 g         1229 t
ORIGIN      
        1 tatctcatat aaacatctta ataatagctg atataaacaa caaagatatt tgtttaaagc
       61 ttaatataaa atacatgttt aatttaataa atataaatag acaactttat ccagctgaaa
      121 atagtaaaaa taatgataaa aaagagggga tgaagaataa tatagattca agtatattat
      181 tagctgatga ccttttgagc atttatagat tattaaaaaa gataaaaata catgaacttt
      241 attcaaatat taattttgga actggaaata ttggcttaac atcttctgta tatgtattga
      301 taaatacttt gtatggaaat ttatttaata tgattgatgc agaaaaaatg tatttaaatg
      361 taaatccaga ttttacaaaa gattatgttt taggaaatat aaggatacat ataaggccaa
      421 gaataaaagc tttatttaat attataataa tgattaataa aattatgaat aaaaataaag
      481 gaaataagga aggtgatagt aatgagagca acaggttcga tacaaagtct tatggaaaca
      541 actcttgaaa caattaaagg ttctatagat gcaaatacta ttataggaga ccctataaaa
      601 acagatacta cagtggttgt acctatatca aaggttacaa ttggctttgg tataggagga
      661 ggagaatact caaaaggata tgatgataaa gatagggaaa ttgaattaaa aaatgaaaaa
      721 tcagatacga attttgctgg aggtagcgca ggggctatat ctgtacaacc agtagcattt
      781 gtagtagttg aaagtggaga gactagaata atgagtttag acagtaatat caatctagtg
      841 gataatatat tatctataac tcctagagta ttggagaaaa ttcaaaatat atcacaaagt
      901 aataagaatc aagataaaaa caatatgtaa gaataaaata tttttaaata tgtatatagt
      961 tttaagtaaa gtagtggggt tagtataaat cccactattt tgttgtgtac tttaaacatt
     1021 tatatctaat aagattttag tttaaaacaa tttaaataga taataaagtt gatatatagt
     1081 ataattataa aaagaaaaga agtattatat tatagatata taaacgcatt tgagatataa
     1141 gagggagtta taaatcatta gttttgaggt gaatgttatg agcgataaag aattatctat
     1201 tttgttgtta gaaagtatga ataattttca taatttaata aagattatta ataacgaaag
     1261 atataaaaaa gataaggttc ttacagaaag acagttcttt gccttagtaa aaataagaaa
     1321 acatgataaa atagagctta aaaatctaag tagagattta catgtatcta cttctagtct
     1381 gtgtatacta cttaataaat tggttgagca agagtatgta tatagggaag aagatagtag
     1441 agatagacga aatacttttt atggaataac taaaaatgga gaaaagatat tggataatga
     1501 aatactaaag tttgtatcta taataagtga taaaatggat tgcttagata tagataataa
     1561 agataaatta tttacttctt tggaagagtc taaaaatata atagaacaat tattttaaaa
     1621 aggagaattt attatgaata taaagtattc taaagagata ttaaaaagtt tagaaaatgc
     1681 tataacagta gtagctctat ttgagggaga agatgtttct acgacatttg ataaaataaa
     1741 tatgataagc agaaatttta attctaaaga aaatagtata gatactgtaa cattggttga
     1801 aaataacaac ttggtaaatg tttgtcttgt tggttttggt aagagagagt tattgaatag
     1861 agaaaaaatt agaatcatag gtggtaattt gtgtaaaaaa ttaaaaaata tattgaaaaa
     1921 tgaagcatat aagagtagtg atgttaatgt acttaatctt aatttaaata gtgatgaaat
     1981 aggttctttt gtagaaggga tattacttgg aaattataag tttgataagt ataagacaaa
     2041 aagtaaggat tttgaaagaa aagaagttga tactataact atatttactg aaaaagaagt
     2101 tgagtgtcaa atagaaaagg ctaaaatatt agctaattca actataatag ctagaaatct
     2161 agttaatgaa ccatcaaacg taatatatcc aaaaacatta gcaatagaaa cagttaaact
     2221 tggtgcagag ttcgggttta atgttgatgt ttatgaagaa gaaaaaataa aggcattggg
     2281 aatggatgct tttttcgccg tttcaagagg ttcaatcaat aaaccaagat tcatagtaat
     2341 gagatacttt ggagataaag aaagtacaga tatattaggt ctagtaggta aaggattgac
     2401 ttatgatact ggtggatatt ctttaaaatc aaatgcttct atgctagata tgaagacaga
     2461 tatggctggt gctgcgagtg ttataggtgc tatgtgtgct atatctcaaa gtaaattaaa
     2521 gaaaaatgtt atagcagtag tggctgcatg tgagaatgct ttatcaggag gttcatacaa
     2581 acctggagat ataattagtt ctatggcaaa gaaaactatt gaagttttaa atacggatgc
     2641 cgaaggtaga cttacattag ctgatgcaat atactacatt ataaacaatg aaaaagtaac
     2701 aaaggtagtt gatgtagcta cattaactgg tgctgcttta acattgttag gaaatgttgc
     2761 aactcctata gtaacaaata atgatgattt ttattgtgaa ttagaaaaag ctgccacttt
     2821 atcaggagaa agggtatgga agatgccaat ttatgatgaa tttaaagata tgataaaagg
     2881 agaagaagca gaccttaaaa acactggtgg taaaaatgct ggatgtataa cagcaggagc
     2941 ttttattggt gaatttgttg ggaatactcc ttggatacat atggatatag cggggacaag
     3001 tacatcatca aaatcaattg gatacaaagc aaagggggct acaggagaac ctgttagaac
     3061 attatactat ttggcagaaa tctaaaaaac aattataaaa tataatttat aaagccacat
     3121 ggatattttt gtaatttaag attatctatg tggctttatt ttttatttaa gtaaattatt
     3181 tactcttttt ctttttctca agtataaaag tatctctatt aaataaaaga gcaggtatgt
     3241 acataatact agtaacaaaa ataaacgtaa aaatagcagt tgtgctatga cttaaaaaac
     3301 cataatagta ttgtgagttg gtaaatgtgt ttagtaaaaa tatgaatgaa aaataaataa
     3361 aacatatagc taagttatct ctaagtgcat atctgttaac tctaaatttt ctaacatcaa
     3421 ccatatatat atctgcataa attatcaagc acttagagaa gataaatata aaatatatta
     3481 agctaggaaa ttcatagccc aagtttttag aaaaaatgag ttctccaact gaacaaacta
     3541 aactccaaga aaaaaatata ttaaaaagat gatactgctt aaaaattagt atggctatac
     3601 acagatatat acagatataa ttgattccaa ttggtaaaga gtttagagta ctataattac
     3661 ctgaagaaat aagtaataat tgctctagag ccataattat tacaagtaat gtacaaatta
     3721 tcttctcaac tgtatagcta taaggtaaaa tatttttggt tagttttgga caatagtata
     3781 aaaataatcc aaatataaag agacttacaa attgttcact agaaaaaaga aaaattttaa
     3841 acatataaca ttctctccta taataaatt
//
LOCUS       DAMJVX010000251         3827 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.258, whole genome shotgun sequence.
ACCESSION   DAMJVX010000251 DAMJVX010000000
VERSION     DAMJVX010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.258"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            84..1574
                     /locus_tag="PC407_003471"
     CDS             84..1574
                     /locus_tag="PC407_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2498877.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            1832..3328
                     /locus_tag="PC407_003472"
     CDS             1832..3328
                     /locus_tag="PC407_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2498878.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACISACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFEFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            3462..3812
                     /locus_tag="PC407_003473"
     CDS             3462..3812
                     /locus_tag="PC407_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2498879.1"
                     /translation="MKKIVLKDILFLVTTLSIIVILLFVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKNTAFKTTILSTGKSDCILIEIGDKFIMIDTGEDKNGEQIVDKL
                     KEKEINALDYLIKK"
BASE COUNT         1501 a          392 c          762 g         1172 t
ORIGIN      
        1 taatatttta tgtatatatt aataaaagga catattgtgt tataatggta tcgtataaaa
       61 tcatttacgg gggtggtatt aaaatgtttc aatttgaaac acaaatacac aaattaagac
      121 atgatgtttt aactgcaata gctagacttg gaaaagaaga taaccttacc agagaagcaa
      181 tggataatat tcagtatgaa ataataaaag gtgagaaacc tacatacaga tgttgtgttt
      241 ataaagaaag agctataata tctgagagag taaatgtaag tatggggtta caaccaggtc
      301 aagataagat agatttagat acaatggata tagaagaaga tgaacaaatt atatatattt
      361 tggaagctgc atgtgacagt tgtccaataa atgaatttac agtaacagat atttgtagag
      421 gatgtctagc acatagatgt aaagaagctt gtaaatttgg agctataaca catgtcggag
      481 gaatggccta tataaatcat gaattatgta aggcttgtgg aatgtgtaaa aaagcttgtc
      541 aatatgatgc catctcagag gttgttagac catgtaagag tgtttgtcca actaatgcct
      601 taggatttga tagagaaaat atgaaagcta tgatacatga ggataaatgt ttaaactgtg
      661 gagcatgtat gtcagcatgt ccatttggag caatatctga taagagtttg atagctcctg
      721 tagctagaaa gttggttcaa aaagagaaaa tgtatgcggt agtagctcca gctataacag
      781 gtcaagttga agcaaatgtt acatatggtc aaataaaaaa tgctataaaa tcactaggat
      841 ttgttgatgt attagaagca gcttgtggag cagatgcagt tacagttcat gaaagcttag
      901 aatttgctga aagaatggaa aagggagagg cttttatgac aaattcttgt tgtccaggat
      961 ttgttggtta tatagaaaaa acatacccaa atcatgtagg aaaaatatct tctacagtgt
     1021 ctcctatgat agctagtggt agatttataa aaagtcatga accagatgct aaagttgtat
     1081 tcataggacc atgtacagcc aaaaagagtg aggcagctaa gaaagaatta aaaggagctg
     1141 ttgattatgt acttactttt gaagaaataa tggcattatt tgaggcattt gatatagact
     1201 tggctaagtg ttcaaatgaa gatatagatg atgcatcaat ttttggaaga ggttttggag
     1261 ctgctggtgg tcttacaaaa gctatagaaa attatcttgc tgaaaaaggt attggtgtta
     1321 attttgaacc tgtaaagatt tctggttcaa gagaaatcaa gaaaactatg actatggcta
     1381 atttaggaaa gctagaaggt aattttatag aaggaatgat gtgtgaaggt ggttgtataa
     1441 atggttctgg taaattctta tcaggtgcaa aggttaaagc cacttttgat agaaaaaatt
     1501 ctcaatcaac taagaaaagt gtcttagcaa atacaaagat aaaagatttt agtaatataa
     1561 atttagaaaa ataaagataa tacaaagcaa caaaaataca tattgttaat agagttgttc
     1621 taagttgagg tttaactttt ggacaactct atttttattt atatatttaa ttaatgaaca
     1681 actaaaataa aaattacata tataatattt tgtatataac ttaacaaagt atatgcctat
     1741 gttataataa tttatagaaa taaataaaaa atacaatata cataatagta gaaatatagt
     1801 aaaaaggtgt gatttatagg gaggattact tatgtttgaa tttgaaacgc aactaaaaag
     1861 gttgaaacat gaagttttaa cagcagtagc aagactatct attgaagaaa atttaacaaa
     1921 agaaaatata gaaaaaattc catatgaaat aattaaagga gaaaaggcga gatatagatg
     1981 ttgtgtttat aaagagagag aagtagttct tgaaagggcg caacttgcat taagtttaac
     2041 tccaaatagt aaatatggag atataaatcc agattcagtt agtttggaca cagatgagca
     2101 aattatatat atagttgaag cagcatgtga tagatgtcca ataaatgaat ttacagttac
     2161 agagctttgt agagggtgtt tggctcatag atgtaaggaa gcatgtaaat ttggggcaat
     2221 ttcttatata aatgggagag catatataaa tcatgattta tgtaaggctt gtggaatgtg
     2281 caaaagttca tgtcaatatg atgctatatc agaagttgta agaccttgta aaagcgtttg
     2341 tccaacagga gctttggatt ttaatagaaa cactatgaaa gcaatgatac atgaagataa
     2401 ttgtataaat tgtggagcat gtatatcagc atgtcctttt ggagcaatat ctgataagag
     2461 tttgatagca cctgttgcaa aaaaacttgc aaagaaagaa aatatgtatg caatagtagc
     2521 tccagctata acaggtcaaa tagatagtaa tattacatat ggtcagttaa aaaatgctat
     2581 aaaatcactt ggatttgtag atatgataga agcttcttgt ggagcagatg cagttacagt
     2641 tcatgaaagc aatgagctta ttgaaagaat aaaatcagga gataattaca tgacaaattc
     2701 atgttgtccg ggatttttta agtatataga gaagatgttc ccagatgaaa aagatagaat
     2761 atcatctaca gtgtctccta tggtagcaac aggaagatta gtaaaaaaaa tagattctga
     2821 tgctaaagtt gtatttatag ggccatgtac agctaaaaag agtgaggcaa cagagaaaag
     2881 tttaaaagat tcagtagatt atgtacttac ttttgaagaa ctaatggcat tatttgaagc
     2941 atttaatata gatttatcaa aatgttcaaa tgaagatata aatgatggtt cgatttttgg
     3001 aaggggattt ggagcgtctg gtggacttgc caaatcagta cagaactata ttgctgaaaa
     3061 gggacttagt tttgagtttg aacctataaa agtatctggt ccaagacaaa ttaaaaagac
     3121 aatgacaatg gctaaattgg gaagattaga aggtaatttt atagaaggaa tgatgtgtga
     3181 agaaggttgt attaatggag caggtaagtt tttatcaggt gtaaaagcta aaaatacttt
     3241 tgatagaaaa aatggacaat ctactaaaaa gagtgtttta tctaatgata agattaaaga
     3301 atttgaagat gtaaatttag aaagataatt gttttggtac agtcattttt ttatcatgcc
     3361 acaaatatgc ctaaaatttt ccctttttat atgtgtattt tacagagtta aggttgctat
     3421 actataaata tgtaataaat aaaatttata ggagcttttg gatgaagaaa atagtattaa
     3481 aagatatcct gtttttagtt actacattat ctataatagt aatattattg tttgtaacaa
     3541 atataactac taatagtaag ataaaatcaa caagtgaatc ttatttaaaa agtgacaaaa
     3601 atagtgtgag gcaaaatggt atccagagga gcacaaaaaa tactgcattt aagactacta
     3661 tattaagtac tggaaagtct gactgtatat taatcgagat aggtgataaa ttcataatga
     3721 ttgatacagg agaagataaa aatggagaac aaatagttga taaattaaaa gaaaaagaaa
     3781 taaatgcact tgactatttg attaaaaaat agctacgcac actcgtg
//
LOCUS       DAMJVX010000252         3799 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.259, whole genome shotgun sequence.
ACCESSION   DAMJVX010000252 DAMJVX010000000
VERSION     DAMJVX010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.259"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            83..1195
                     /locus_tag="PC407_003474"
     CDS             83..1195
                     /locus_tag="PC407_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2498880.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1403..1879)
                     /locus_tag="PC407_003475"
     CDS             complement(1403..1879)
                     /locus_tag="PC407_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498881.1"
                     /translation="MVKKRLVAVVLSLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQPNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2078..3703)
                     /locus_tag="PC407_003476"
     CDS             complement(2078..3703)
                     /locus_tag="PC407_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2498882.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
BASE COUNT         1318 a          611 c          424 g         1446 t
ORIGIN      
        1 tgtatgcaac tctatttcaa aattattttg tgatatatat atgttataat aaatgtataa
       61 aattttgatt ggtgggttat atatgaatgc atttattaga aaaagaaata aaaactatgt
      121 tgtttacctg gaatttgaag atgaaaagac aggtaaaaga aaacaaaaaa atatgggtac
      181 ttttgataaa aaaagggaag caagtaaaag actaaatgag gtaaaagaaa gcttatataa
      241 tgatggtttt ttagtaccaa gcgaaattac agtgtcagaa tttctattag attttctaaa
      301 taaatatagt gaaaatatat ctttagctac atataataat tatgttagta tttgtaaaaa
      361 ttacattaat ccatctattg gaaaatataa aatacaagac ttacatccgg ttcacataca
      421 aaactacata gataaactat cttataaact aaatcctcaa tcaattaaga ttcatataaa
      481 tatattgaaa cttgcaataa aaagagctta tagactcaaa ttaataaaag aaaatgttat
      541 ggatagtata gaggctccaa gatataaaaa atttaaaaat gagatttatg atagagaaca
      601 aatgataaaa ttattagatt tagcaaaaaa tacagaaatg gaattaccta ttaatttggc
      661 tattggatta ggtcttagaa tttcagaagt tttaggtcta acttgggata atattgattt
      721 tgaagaaaat acaataacag taaataagat aacttcccga attaatggtt ctgttattct
      781 caaagagcca aagacagaaa gctctgttag aaaaatatct tctcccaaag aattaatgtc
      841 tttattaaaa gaatataaaa taaaacagaa taaaaattta ttaaaatctt ccattagaaa
      901 taataataat ttgttgtttt tcaataagaa atgtgaacct attgctgaag atgttatgag
      961 taaaaaattt aaaagatttt tagaaaagaa tgaattacct catattagat tccatgattt
     1021 aagacactca catgttactt tacttataaa ttctaaagta cctataaagg ttatatccga
     1081 gagagtagga cactcaaata taagtactac tctaagcgtt tattctcatg tacttaaaga
     1141 aatggataaa gaggcttctg acaaaatttc tgaaagttta tttaatgcta attaatatta
     1201 atttatctta gctttaaatt agcttagctt attgagatag attcattaat attaaatgta
     1261 tctatctcaa attaataact acttaaataa tctgttttat tttaatttat cgcctcagtt
     1321 attatacttt tttatgaaaa attatacttt acaataaaaa agatatttcc ttatagaaag
     1381 tatcttttta aatactattt tattatatac ttgctaacat tggaatatct aacatcttct
     1441 tcattacatc atctactttt tttgctttat attcattgta tccaattaaa tatccttttt
     1501 catatgaact ttttaaaatc tctttttcat ataaattatc agatttggct tctgtgacac
     1561 ttaaattata aagcccttta cacccatctt cataaccttt attataccct ttttctgcaa
     1621 aatagttttt atgatttttt tcgttatgta ctgaagatat tgttactcca gaattttctt
     1681 ttgtactagc ataaggacaa aaacctttat ttggttgatg tgctgaatgt cctccacaat
     1741 aataacgtgt taactctgat ttatttttat tatgatgacc tcctgatgaa ttatgccctc
     1801 ctgagtgagc atatgaaaaa ttccctacac taatcatagc taaactcaat actacagcta
     1861 ctaacctttt cttaaccata atctattccc cccaaaatgt tatatatttt tattatatca
     1921 tctactatat tatatttaaa ttcttaatat acaaaatatt actcgattct gctaataaac
     1981 atatttaaac aacataaaaa acctcctaat atttgattaa aaatttacac actcatgcat
     2041 agcatgtatc aaatattaaa aggttttata ctaataatta tttatctttt ccatacattt
     2101 tagctaattg gtcttgtatc tctttacttt ctaaatactc atcaaagtcc atttttctat
     2161 ccattattcc atttggagtt atctctataa ttctatttgc tatagtagat ataaattcat
     2221 ggtcatgaga agtaaataaa agcactcctg ggaacttttc aagtccttta tttacagaag
     2281 ttatactttc taggtctaag tggttagtag ggtcatctaa tacaagtaca tttgcatttg
     2341 aaagcattag cttactaagc atacatctaa ccttttctcc tccagatata acttgagctt
     2401 cttttaatgc ttcttcacct gagaataaca ttctacctaa aaatcctctt ataaaacttt
     2461 cacttttttc ttcagagaac tgtctaagcc agtcaaccaa agaatattct actccgtcaa
     2521 aaaacttatt atgatttttt ggaagataat cttgagaagt agttactccc catttatact
     2581 ctccactgtc tggttcaagt tctcccataa ctatgttgaa aagtgcagta gtagctattt
     2641 catctcccat aaatactact ttatcatctc tatccaatct aaatgacaca ttatcaagaa
     2701 ccttaacccc atctatagtt ttagttaaat tgtgtacttc taaaacttca ttcccaattt
     2761 ctcttgcagg tgtaaatcct acatatggat atcttcttct tgatggttgt atatcttcaa
     2821 cttctaattt ctctaactgt ttctttcttg atgttgcttg tttagccttt gaagcattag
     2881 aactaaatct agctataaat tcctttaatt gagctatctt ttcttcagtc tttttatttt
     2941 ggtccttagc cagttgtaaa gccaattgac ttgactcata ccagaaatca tagtttccaa
     3001 cgtacatttg tattttacca aaatctacgt caactatatt agtacatatt tgatttagga
     3061 aatgtctatc atgtgatact attataacta tagactcttc taaatccatt ataaaattat
     3121 ttaaccagtt tattgattta aaatctaagt ggtttgtagg ttcgtccatt aatagtattt
     3181 caggcttacc aaaaagtgat tgtgctaaca ataccttaac cttttcccca ccaactagtt
     3241 ccttcatttg tttgtaatgc atatctttcg ttatgccaag acccataagt attttctcag
     3301 cattagtttc tgcatcccat ccgtcaagct ctgcaaactc accttcaagt tcggcagctt
     3361 ttatcccatc ttcttcatta aagtcttcct tcatatacaa tgcatctttt tctttcatta
     3421 tgttccaaag tctctcatga cctcttataa caacatttaa aacagtttct tcttcatatt
     3481 caaagtggtc ctgttttaaa actgacattc tttctttatc agttatagat atactacctg
     3541 tattaggctc tatttctcct gataatattt ttaaaaaagt agattttccc gcaccattag
     3601 ctcctattat tccatagcaa ttacctttag tgaattttaa gttaacatct ttaaatagtt
     3661 ccttatcacc aaatctaagt ccaacacctg taacttgtaa cattaatata ttttcctttc
     3721 tttttgctca taatttattt tatcataaaa ctttgataac atttttatta tatattatca
     3781 cgtaaatagt atatttttt
//
LOCUS       DAMJVX010000253         3794 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.260, whole genome shotgun sequence.
ACCESSION   DAMJVX010000253 DAMJVX010000000
VERSION     DAMJVX010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.260"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            235..384
                     /gene="rpmG"
                     /locus_tag="PC407_003477"
     CDS             235..384
                     /gene="rpmG"
                     /locus_tag="PC407_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDF2498883.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            410..631
                     /gene="secE"
                     /locus_tag="PC407_003478"
     CDS             410..631
                     /gene="secE"
                     /locus_tag="PC407_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDF2498884.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            651..1193
                     /gene="nusG"
                     /locus_tag="PC407_003479"
     CDS             651..1193
                     /gene="nusG"
                     /locus_tag="PC407_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HDF2498885.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDVDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1229..1654
                     /gene="rplK"
                     /locus_tag="PC407_003480"
     CDS             1229..1654
                     /gene="rplK"
                     /locus_tag="PC407_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDF2498886.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1724..2422
                     /gene="rplA"
                     /locus_tag="PC407_003481"
     CDS             1724..2422
                     /gene="rplA"
                     /locus_tag="PC407_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDF2498887.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2474..2602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2645..3151
                     /locus_tag="PC407_003482"
     CDS             2645..3151
                     /locus_tag="PC407_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDF2498888.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3209..3574
                     /gene="rplL"
                     /locus_tag="PC407_003483"
     CDS             3209..3574
                     /gene="rplL"
                     /locus_tag="PC407_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDF2498889.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
BASE COUNT         1536 a          448 c          754 g         1056 t
ORIGIN      
        1 atggattaaa aggaataaca aaaagagggg ttaggctcct ctttttttaa catagaaata
       61 caaaaacatt gacacagcat aggattttgt gataacttat acaaggtaat gattatagga
      121 agttaataat atcttgaata ctgtagattt ttaataaata catattttaa agatattata
      181 ttcacatata ttaacaatat atattattgt attacaaagt tggaggtgta tcagatgaga
      241 gttaaagtaa ctttagcatg tacagagtgt aagcaaagaa actacaacac tactaagaat
      301 aagaaaaata atccagacag aatagaatta caaaaatatt gtagattctg taaaaagcat
      361 acaactcata aagaaacaaa atagtttttt ataaggatgt gagtatggaa tggctgccca
      421 aaaaaatgac ggtgcaaaaa ctaaaaaaag gtttagttta tttggatatt taaaagaaac
      481 taagcaagaa ttaaaaagag ttacatggcc gacaaaaaag gaactgttta aaaacacaag
      541 tatagtttta actgtagtta tttcatgcac tatattagta tggggtatag acactatatt
      601 atctggtgca ctagcgttat tactaaaata gacgaaaaga ggtaagttaa atgtcagaat
      661 tacaagaagc aagttggtat gtagttcata cctattcggg acatgaaaat aaagttaaag
      721 ctacaattga gaaagcagtt aaaacaagag gtatggaaga ctgcataaga caagtagttg
      781 ttccaacaga agaggtagtg gaaactacta aaactggaaa agaaaaaact agacaacgta
      841 aagtttatcc aagttatgtc ttggttaaaa tgataataac tgatgaatct tggtatgtag
      901 taagaaatac taagggagtt acaggtttcg ttggacctgg ttcaaaacca gtaccattaa
      961 gtgaagatga agtcaaagct atggggatag atacaacaga ccctaaagta gtaaatagcg
     1021 atgttgattt tgaaatagga gacacagtaa aagtatctca aggaccattt agtggacaaa
     1081 taggtaacat agaagaaatt gacttagaaa atagagaagt caaagtatgc ataaatgcat
     1141 ttggtaaaag gactctattt gtaatagagc ttgaaggtat agaaaaaatc taatactata
     1201 tagaaaaatc tgaggaggtg cgctcaaaat ggctaaaaaa gttataggtc aaataaaatt
     1261 acaaatacct gcaggaaagg ctactccagc gccaccagtt ggaccagcat taggacaaca
     1321 tggtgttaat ataatgggat tcacaaaaga atttaatgct aaaactgcag atcaagctgg
     1381 aatgataata ccagttgtta taactgtata tcaagataga tcattcagtt ttataacaaa
     1441 aactccacca gctgcagttt taattaaaaa agcattaaac ttaaaatcag gttctggaga
     1501 accaaacaaa aagaaagttg ctaaaatgac ttcagctcaa gtaagagaaa tagctgagtt
     1561 aaaaatgcct gacttaaatg ctgcatcagt agaagctgct atgagtatga tagctggtac
     1621 tgctagaagc atgggtgttg taatcgaaga ctaattaaaa gatacacttt ttttataaag
     1681 tgggaggcga gaagccgaaa ttaaccacaa ggaggaaaat aaaatggcta aaaaaggtaa
     1741 aagatatgca ggtgcattac aaaaagtaga tagaactaaa ttttatgatg catcagaagc
     1801 attaacatta gtttcagata tagctggagc taaatttgat gagacagtag aagcacacat
     1861 taaattaggt gttgactcaa gacatgctga ccaacaagta agaggtgcag ttgtattacc
     1921 tcatggaact ggtaaaacaa aaagagtttt agtttttgct aaaggtgaaa aagctaaaga
     1981 agctgaacaa gctggtgctg actttgtagg agctgaagaa ttagttcaaa aaatacaagg
     2041 tgaaaactgg tttgactttg atatagtagt tgctactcca gatatgatgg gtgtagtagg
     2101 tagattaggt agagtattag gacctaaagg tttaatgcca aaccctaaat caggaacagt
     2161 tacatttgat gtagctaaag ctatagatga aataaaagct ggtaaagttg aatacagatt
     2221 agacaaaact aacataatac acgttccagt aggaaaagta tcatttggtg gagaaaaatt
     2281 aactgaaaac tttactgcat taatggatgc tataataaaa gctaaaccag ctgctgctaa
     2341 aggacaatat ttaagaagta taacagtagc ttcttcaatg ggacctggag ttaaaataaa
     2401 cccagcaaga acagctgaat aataaaactg ttgacacagg ttgacaaaaa tgttattata
     2461 tcacttgtca atgtaaaaaa gaatagattt gccgtagata gtgggtgcga aagcttaata
     2521 tcccgccgag gttttagata aattgagatt actaaagctt ttgatgtcta cttttggcaa
     2581 caaaagcttt ttataatgtt gcggtaaata ttaagcctat ttggtgaagg aggtgcacat
     2641 ctatatgaga aaagctatag aaattaaatc agaagttgtt tctgagatag ttgaaaagtt
     2701 acaaaaatct tctgctgctg ttgttgtaga ttacaaagga ttaacagttg aagaagtaac
     2761 tgagttaaga aaacaaatga gagaagctgg tgtagattac aaagtataca aaaatacttt
     2821 agttagaaga gcagctaaag aagttggtat agagcaattt aatgatgaat tattagttgg
     2881 aactaatgcc atagcatttg gatatgatga tccagttgct ccagctagaa tattaaaagg
     2941 attcatggat tctcatccaa aaatgaaatt aaagatgggt attgtggaag gtgcattcta
     3001 tgatgaatct aagattgttg aaatggctaa cataccatca agagaagttc ttatcgctaa
     3061 attacttggt agcttaaaag ctccagtatc aaacttcgca tacttaatag atgcaatagc
     3121 gaaaaaggca gaaggtcaag aagaagctta attgcttaac taaaaaatta taaatattaa
     3181 tttgaaataa aattcggagg tgctaaaaat gacaatagaa caaatattag aagctataga
     3241 aaatatgaaa gttttagaat taaatgaatt agttaaagct gctgaagaaa aattcggtgt
     3301 atcagcttca gctccagtaa tggtagctgg tgcagctgct ggtggaccag ctgctgaaga
     3361 aaaaactgag tttgatgtag tattaactga cgttggttct tcaaaagttg gagttataaa
     3421 agcagttaga gaaataacag gattaggatt aaaagaagct aaagaagtag ttgacaatgc
     3481 tcctaagaca gttaaagaag gagcttctaa agaagaagct gatcaaataa aagagaaatt
     3541 agaagctgct ggagctaaag tagaagttaa gtagtttctt taaaaagata cccattgggt
     3601 atcttttttt atttataaaa aactataaag aaagtatttg gaattataaa gaaagtgttt
     3661 ttataaaata aaaaaaattt atataatagt actaaattaa gggtatcata tatggtgtaa
     3721 tggtatttta atttaacatt attatcaaat taaaatataa acaattgttg taatgtctgt
     3781 atataaagta tata
//
LOCUS       DAMJVX010000254         3762 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.261, whole genome shotgun sequence.
ACCESSION   DAMJVX010000254 DAMJVX010000000
VERSION     DAMJVX010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.261"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     misc_binding    318..556
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            620..2539
                     /gene="thrS"
                     /locus_tag="PC407_003484"
     CDS             620..2539
                     /gene="thrS"
                     /locus_tag="PC407_003484"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDF2498890.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            3307..3735
                     /locus_tag="PC407_003485"
                     /pseudo
     CDS             3307..3735
                     /locus_tag="PC407_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1468 a          399 c          689 g         1206 t
ORIGIN      
        1 tacttattaa ctatattaag tattgattgt atttaagtat acaaaaccta aaaagagtac
       61 aaaaattaga agttagtttt aataaaaatt agttttagta tttatctagt catatctatc
      121 atgcatgtgg atacgtttta aaaattttaa atttatgaaa atattataat atcactataa
      181 atcaagagat taatttaata taaaaatata agaatattgg tttacagctg attgagatga
      241 aaaataatgg gtaattaata tattacatta atgttgattg atgtataact atctgttata
      301 atgaaaatat attaaaaatc atagtgttga agaggaagag tagagtagaa accattctca
      361 gagaatttgg gtctatggct gagagcccat gtaataggtc tatttgaaaa ctaccttgga
      421 gctgagctta ttagcagtat ttgtatttgt actatacgtg aaagtgctcg ggtgacgacc
      481 ttatagtcat caagagggtt gtatatactt gtatacgcca acttgggtgg aaccgcgaat
      541 taatatcgtc ccaaacatat ttggggcgat tttttattac aaaaaatcta aaaattattt
      601 caataaggag gaaaaaacaa tgattaaagt tgctttaaaa gatggctcaa taaaagaatt
      661 tgaaaatgct atttctgtta tggatgtagc taaatccatt agtgaaggat tagctagaaa
      721 tgtggtagca gcgtctgtga acggagaggt tgtaggtctg gaccacatta tagatactga
      781 ttgtgacttg aatttattta agtttgaaga taaagaaggt aaggaagttt ttagacatac
      841 atcagcacat atattagctc aagctataaa aagactttac ccagaagcaa agcttgctat
      901 aggaccaagt atagaaaatg gattctacta tgatatagat ttagaccata gattagttcc
      961 agaggaccta gagaaaatag aagctgaaat gaaaaaaata gctaaagaag atttaaaaat
     1021 agaaagattt gaacttccta gaaatgaagc tttagagctt atgaaagaac aaggagaaga
     1081 ctataaggta gaacttatat ctgatttacc tgaaagtgaa ataatatctt tctacaagca
     1141 aggtgatttt actgatttat gtagaggacc acatttacca tctactaaaa aggtaaaagc
     1201 tgttaaatta caaagtgtag caggtgcata ttggcgtggt gacgaaaata ataagatgct
     1261 tcaaagaata tatggaactt cttttgagaa aaacaaagac ttagaagagt acttacattt
     1321 actagaagaa gctaaaaaga gagaccacag aaaattaggt aaagaattag gattatttat
     1381 gatacctgaa gaaggtccag gattccctat gtttttacca aaaggtatgg aacttaaaaa
     1441 tgaattatta aaattctgga gagaaataca cagaaaagct ggatatattg agatagaatc
     1501 tccaatcata ttaaatagaa aattatggga gacttcagga cactggtatc attacaaaga
     1561 aaatatgtat acagttaaaa tagatgatga ggattatgct ataaaaccta tgaactgtcc
     1621 tggtggatta atttactata actctcaact acattcatat agagatttcc caatgagagt
     1681 tgcagagctt ggtagagttc atagacatga gttatctggt gcattacaag gattaatgag
     1741 agttagagca tttactcaag acgattcaca tatattcatg ttaccagagc aaattaagga
     1801 cgaaataaaa ggagtagcta acttaataga tggtatctac aaaacttttg gatttgaata
     1861 taatttagaa ttatctacaa gaccagagaa ctctatggga agcgacgaag agtgggaagc
     1921 agcagaaaat ggattaagag aagctttaga agagttaggt ctaccatata ctattaatga
     1981 gggtgatgga gcattctatg gtcctaagat agacttccat ctaaaagatt gtttaggaag
     2041 aacttggcaa tgtggtacta tccaattaga tatgcaactt ccaagacaat ttgataatac
     2101 ttatattggt caagatggtg aaaagcatag accagttatg attcataggg ttgcttttgg
     2161 tagtatagaa agatttatag gtatattaat agagcattat gctggtaaat tcccagtttg
     2221 gttatctcca actcaagtta agatacttcc tatatctgat aaatttatgg attatgcaaa
     2281 tgaagttaag aaagagttat ttgataaggg tataagagtt gaattagatg atagagctga
     2341 aaaaattgga tttaagataa gagaagctca gcttgaaaaa gtgccataca tgttaatagt
     2401 aggtgaaaaa gaagtagctg ataataatgt ttctgttaga tcaagagata aaggtgaaat
     2461 aggtagtatt aaattagatg aatttatagc tagtatttca aaagaaattg aatcaagaga
     2521 aagtattatt caagactaat atataatata ttagtagcta agatattgtt atataagata
     2581 agtttaatca atatgttgat atgttgatac atagtgatta aatttatgaa aagatgagta
     2641 taattagagg tacagttaat catattttta tgattaatgt acctctagtt ttttgtatgg
     2701 tagatttatt aataaatata tcatagaagt gttttgaaca tggaaatatt tatgtgcatt
     2761 ctttaaataa aagtttattt tttgaatgat aattaagtgc ttgttggtaa ggttatggct
     2821 gtaaaaatca taactttatt tttatatttg ttccattttc ataaaaatta ttatcccttt
     2881 aagaaaattt cttaatcctc ttgaaatatt tcctttttag tctaaagtta aatttctttc
     2941 ttcaaaatga atatttaaat gaactaaaat ttaagtacat tttctaatct tatgagtcta
     3001 tttttcatag ataattgccc tataatagaa gaatctatgt tggggctatg tggtgacaat
     3061 aacagcattg tcagtgatta attaatgctt tataaatgat taattagtgt tttatgttat
     3121 ttttggactt accctctata catagattaa tttatttaac tttagatata gagagaatgt
     3181 caagtacata cacagacatt agagtttaag aaagtgttta tggagcaaga acttttaaaa
     3241 gaagtagtga cttagtttaa tttcaagaaa tgataaatac aaaagaggaa aaagagaggt
     3301 gttgaaatga tagaaaaagt agaagaaaaa tctaagatta ttaaaacatc aaaagaatat
     3361 aagcgaacta taattgaaga aaaagataaa gaatggacca aaaaattaaa tgatatactt
     3421 agtagaagta gtttttgtaa tgatgaatta gataaaatca aggcgttaat tccaaaagag
     3481 attttgacta atgaaaatgt tggagaagtt gtgactgaag atggatatgc taagcccgag
     3541 tatgatgagg tatttaaatc attgttcact ttaaataatg aatatgattt acttgtaaat
     3601 tttataaatg atattctaaa agatgctcca tatgctaaca ggaatatcaa accgttcact
     3661 caaataaaga gaataattag agttgaaact gatccaacaa ttaattacat tggagagaaa
     3721 agacctagac ttgattaaaa aatagctacg cacacttgtg ac
//
LOCUS       DAMJVX010000255         3746 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.262, whole genome shotgun sequence.
ACCESSION   DAMJVX010000255 DAMJVX010000000
VERSION     DAMJVX010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.262"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            312..1688
                     /locus_tag="PC407_003486"
     CDS             312..1688
                     /locus_tag="PC407_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDF2498891.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1999..2670
                     /locus_tag="PC407_003487"
     CDS             1999..2670
                     /locus_tag="PC407_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDF2498892.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2861..3658
                     /locus_tag="PC407_003488"
     CDS             2861..3658
                     /locus_tag="PC407_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDF2498893.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         1432 a          410 c          685 g         1219 t
ORIGIN      
        1 tttaacttca aaaaagtata tgaaatttat actggtaaaa tgataaataa attttaagta
       61 caaaatagta aaagctatga tagttttaga cccattatat gtaaaaaata tagatgtttt
      121 tggtgcaatt taaattaaaa gatatatata aaaatttaaa atattccaaa cagaaattat
      181 aaaaattact atcattgtct ttatgcaata tttatactat aatagaataa taatttagag
      241 gaaagctcta aaaataaaca aattaagggt cttaacagta gtgattttat gttaaggagg
      301 tataaagaaa gttgaaaaca atagttagaa gacttattct ctacgcagat ggaatgcgtc
      361 ctactcaaat aatggtatct gggtttgctg cgattattgt aatcggagca ttgttgttaa
      421 cactcccgat agcgtcgcag agtggagaga gtataggctt attaaatgct ctatttacgg
      481 ctacttcagc ggtttgtgtt actggtctag ttatggtaga tacagcaact tattggagtt
      541 tatttggaca gatagttata ataacattga tacaaattgg tggtcttgga tttatgaccg
      601 tagccacaat gttttcactt atggcacgaa aaaaaataca actaagagaa agattattaa
      661 tacaagagtc attaaatcaa gctgatttat cgggacttgt aagacttaca agatttgtat
      721 taataataac cataacaata gagggaatag gtgcactggt tttatcaact gtctttattc
      781 cacaatttgg tttgtctaaa gggatatggt atagtgtatt tcacgcaata tcagcgtttt
      841 gtaatgccgg atttgacttg atgggcagtg taagtggccc atttacatcg ttaaattctt
      901 atgtaaacaa ctttacagta tcaatgactg tttgcgcact tatagtgctt ggagggctag
      961 gattccctgt tgtacttgat attgtaagaa aaagaagatt ttcaaaatta aatgtgcatt
     1021 ctaaggttgt attgttttca actgctacac ttatttttgt gggtgcatta tttatttttc
     1081 ttatagaatt taatcaaaaa gctactatgg cggacttgcc tttaaaggga aaagtactat
     1141 cagcaatatt tcaatcagta acagcaagaa ctgctggttt taatacactt gatttagcta
     1201 ctttacgaga aagtagtgta tttgtaatga taattttgat gtttattgga gcatcacctg
     1261 catcaactgg tgggggtata aaaacaacaa cactagctgt tttaatcatt acagttagaa
     1321 gttttctatc aggaaaatca gatatagaag cttttgaaag aagattagct ccatcaacta
     1381 taaaaaaatc tcttggtata tttgtaatca gtatatcagc agttattttt ggaacattga
     1441 ttatttcaat aactcagcct aattttacat tagttcaatc agcttttgag gttacatcag
     1501 cacttgctac agttggttca agtttagctg gaactccaaa tttaaatgct ttaggtaaga
     1561 ttataataat aatctttatg tttatgggta gagttggttc tcttacttta tttatggcta
     1621 tactttctgg tggtagaaga aagagtcaac caatcagata tgcagaaggt aaaattatgg
     1681 ttggttaagt taccaaggag taatataaat ttataatgga tttagagtag cactgtaaat
     1741 ctgttgataa aataaatgag cctagatgta attatatgaa gaaataaaat caactaataa
     1801 acaaaattaa ttaatgaaat aaaaaaacag ggaaataaaa tacaaataaa aaggtaaatc
     1861 aaaattaaat taatctgtgt atataaaatt atatattgtg tataagtttt gtatgtgtaa
     1921 agtatataag ttttgtatgt gtaaacaatt aatttaagag atattatata aattgtgtat
     1981 tagggaggaa gtttcgagat gaagcaatat atagtcattg ggtgtgggag atttggaagt
     2041 tcagttgcgt ctactatgca tcttttagga catcaagtaa tggcaataga caaaaatgaa
     2101 gattcagttc aaagtatatc tgacaaggta acccattcac ttatagtgga tgttactgat
     2161 gagcaagcgt taaggtcatt aggtttaggt aactttgatg tagcagtagt tgcaataggt
     2221 tctgatataa gggcatctat aatggcgact cttatagcca aagaaatggg tgtagagttg
     2281 ataatatgta aggcaaagga tgaattacaa gctaaagtgc tttataaaat tggcgcagat
     2341 agagttgtat ttccagaaag agatatggga gtaagagttg cacacaattt agtttcggat
     2401 aatatattag accatattga acttgaccca gagtattcaa ttgttgaaat cgtaactcca
     2461 aatagttggg ttggcaagac acttatagag cttgaattaa gagctagata tgagataact
     2521 gtacttgcta taaaaacagg taaaaatata aatgttacac cttctccaga tgaggaactt
     2581 acagccggaa gtatcctagt tataatcggt caaaatacta gtataacagc gataacatct
     2641 ggaaataagg ggataattag aagaagataa tttactattt aatatatatt taattgcaat
     2701 gaaagtaaag agtatcatat aattatgaat agttatatga tactattttt atttaatcga
     2761 aggtagtatt tatttgtaag attagataaa gagaagttaa attaaaagta aggaggctgt
     2821 gctaatataa aaatttataa gttattagca tttagataaa atgattacaa atataaacag
     2881 taaggataat gaaaagttaa agtatacaag agcactatta aaatcaaaga ataggaataa
     2941 agagtcaaag ttcataatag aaggatacag aatagtaatg cttgcacttg aatgtatggc
     3001 aaaccttgat tatgtattta tcaatgaaga atttgaaaat aagaaagaac atgtaaaact
     3061 attagaagat ttggataaaa aaaacacaaa gatatacaag actactaata aaaactttaa
     3121 agaattagtg gatacagaaa atactcaagg aataataggt gtagtttcat ttaagaaaaa
     3181 aaaattaagt gaaagtataa ataaaaaaga taaatttgta ttgattttag atagaataca
     3241 agacccagga aatatgggga ctataataag gactgctgat tctgctggag tagatgccat
     3301 aatagcacta aagggatgtg tcgatatata caacccaaaa gtaattaggt ctactatggg
     3361 ttctattttt gatatgaata taattgatgc ttcacaagat gaaactgtgg acatgcttaa
     3421 atcattggat tttaatatag tttcaagtta cttaaataca gaaaattttt atgacaaaat
     3481 agattatggt tcaaaagtag cattggtgat aggaaacgaa gcaaatggaa taaatgaaga
     3541 acttgtatca aagtctgata ttttggttaa gatacctata tatggtaaag ccgagtcgtt
     3601 aaatgctgcg ataagctctg ctatactgat gtatgaaata aaaaaatact taatttaatg
     3661 tattgtaata aatataatgt catgttataa tcttgaatta aatagtagag tatcaaaaat
     3721 agtcaatata tattaaaaaa ataatt
//
LOCUS       DAMJVX010000256         3727 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.263, whole genome shotgun sequence.
ACCESSION   DAMJVX010000256 DAMJVX010000000
VERSION     DAMJVX010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.263"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            62..970
                     /gene="trxB"
                     /locus_tag="PC407_003489"
     CDS             62..970
                     /gene="trxB"
                     /locus_tag="PC407_003489"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2498894.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1060..2883)
                     /gene="typA"
                     /locus_tag="PC407_003490"
     CDS             complement(1060..2883)
                     /gene="typA"
                     /locus_tag="PC407_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDF2498895.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3095..3337
                     /locus_tag="PC407_003491"
     CDS             3095..3337
                     /locus_tag="PC407_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498896.1"
                     /translation="MLEVTKIIIKYFASESDTFIKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3485..3676
                     /locus_tag="PC407_003492"
     CDS             3485..3676
                     /locus_tag="PC407_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDF2498897.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
BASE COUNT         1339 a          511 c          613 g         1264 t
ORIGIN      
        1 aaagtataag aatgatgaga ataatatata agaaataata atataaagag aggtaaacat
       61 tatggtagat atcattgtaa ttggtgctgg accagcaggt ttaacttcag caatttatgc
      121 tatgagagca gggttaagcg ttacagtatt tgaaaaaaat atttatggag gtcaagttgc
      181 aagtacctct gaggtagaaa attatccagc agttcaaaag atttctgggg tagaattttc
      241 taataatata tacaatcaag cagttgctca aggtgttgat attcagtttg atgaggttga
      301 agagataaat ttagagggaa aagtaaaggt tgtaaaaact tcttcaggtg aacataaagc
      361 aaaggctgtt attcttgcaa atggagttga aagacgtaaa ttaggttgtg caggggaaca
      421 agaatttaca ggtagaggag tatcttactg tgcaacatgt gatggagcct tttttaaaga
      481 taaagaagtt gctatagtag gtggaggaaa tactgcacta gaagatgctt tatttttagc
      541 taataattgt acaaaagtgt atttaattca cagaagagat agttttagag gtgaagaagt
      601 gttggagaaa tctgtgaaag caagagagaa tattgagata ctttacagtc atggtgtaga
      661 aaaaatagaa ggagaaaaga ctgtatcgaa aatagaagta aaaaacttaa agactgaaga
      721 aaaaagaact atagatgtta gtggtatttt tattgctata ggtcttaaac ctaataataa
      781 aatgtttgaa aatgttcttg atttggatga aggtggatat ataatttcag atgagtcttg
      841 tacaacatca gtagaaggtg tatatgtagc aggagatagt agaactaagt ttttacgtca
      901 aataataaca gcagcatcag atggagctat tgcagccgtt caagcagcta attatataaa
      961 tgttgaataa tataaatttc aaataaaaat agccatataa actgaaataa tttttcagta
     1021 ctatatggct attttgttaa ttagaggtaa atgtacgttt tatttacctt gtctagattt
     1081 gttatatctt aatctatcat gttcatttaa gaatcttttt ctaagtctta tagaatcagg
     1141 tgtaatttca actaattcat catcttctat aaactctagt gcttcttcaa gagtaaatat
     1201 tctaggagga gataatttta tagcatcatc tgaaccagaa gcacgtacgt tactcatttt
     1261 cttgttttta caagggttga ctaccatatc atcttttctg gaattcatac caattatcat
     1321 accttcgtaa acttctacac cagggtctac aaacattaca gctctatcac ttaaggcatt
     1381 aagagaatac cccatagtaa caccaggtcc ttgtgctatt aaaacaccat ttcctcttga
     1441 aggtatttct cctttaaatt cttcaaattt ttcaaatgaa cgaactaaag taccttctcc
     1501 acgagtagcg tttataaatt cacttcgata tcctaaaaga ccacgagtag gtgctaagaa
     1561 ctctattttt acatagtctc cttctattga catagactcc atcatacctt ttctcatgtt
     1621 taattcattt ataatagtac cagagtaaac ttcaggacag tttacaacaa ctctttctat
     1681 tggttccatt agttttccat cttctttatg cattaaaact tcaggtttag aaacaccaac
     1741 ttcataacct tcacgtctca tattttcaag tagtatagat aagtgaagct ctccacgtcc
     1801 agaaactttg tatccatcag tagtatcaag aggttcaact ttaagaccaa catttacttc
     1861 aagttccttt tcaagtctat ctttcaaatg tctagttgta acgaattttc cactttttcc
     1921 aacgaatgga gagtcgttta ctaagaagtt catagaaaga gtaggctctt ctatttttat
     1981 catttccata ggtagtggac tatctaaatc gcatatagtt tcacctatag atatatcagg
     2041 aataccagct ataacaacta tttctccact agtagcttca tcaacttcaa cttgttttaa
     2101 accttcatac acagaaagtt ttgatacttt tcctttagat acaacagaat cttctctaca
     2161 tatagcaact tgtgtattgt ttttaagagt acctttgtaa attctaccta taccaagtct
     2221 acctacataa tcatcatatg caagagctga tatttggaat tgtagaggtt cattatcgta
     2281 attaggataa gcttcaacgt gatttacaat agttttaaat agaggagaaa ggtcttcact
     2341 atcatcatcc atttcaagtt tagcaatacc ttgtttagca ataccatata ttataggaaa
     2401 ttcgcattgc tcatctgttg catttaagtc tacaaataaa tcgaaaactt cattaactac
     2461 ttcttccgct ctctggtctt ttttatcaat cttgtttata aataatattg gttttaaacc
     2521 gaactctaaa gatttttgta aaacgaatct agtttgaggc attgggcctt cactagcatc
     2581 aactaataga ataactgtgt caacagtttt cataacacgt tcaacttcag aagaaaagtc
     2641 actgtgtcct ggagtatcaa ctatgtttat tttgtaatct tcatagttta ttgcacagtt
     2701 ttttgaatat atagttatgc ctctttcttt ttcaaggtcg ttactgtcca taacgcagtc
     2761 tttaactact tcgttctttc taaaaacacc gctttgagaa aggaaagcat caaccaatgt
     2821 tgattttcca gcgtcaacgt gggctataac tgcgatattt ataattttat gtttttgtga
     2881 catttttgtt gctcccttcg attaaaataa cttattaatt atagcacaat atattattag
     2941 gtacaaggag gaatatatta aattgtatta atatagttta aattggataa aactgttagt
     3001 tttgcatctg aaaattattc tgtttaaaaa aattttttag acaaataata atcttaatag
     3061 ataaaaagat taataaaaga gtaaaggatg atatatgttg gaagtaacca aaataataat
     3121 taaatatttt gcaagtgaaa gtgatacttt tataaaagaa gaagtttgta attttgaaga
     3181 acttcttaag tggtctcata aatttattga aggtaaaaag atagaagatg ttttgtatat
     3241 aaagtctaaa gaaaactata aagtaaatgt gattaagtct ttagaaatta aggaattgga
     3301 aatagtgaga aaacaacaga taccaccgta catgtagtat taataatata taaaattaat
     3361 atgtaatact cacaatagga tataaatggt atatatggta ataatttata ttaaaaaaac
     3421 ttttataaag tttatatttg ggtataaaag tattaattag acattaatta ggaggaactt
     3481 aaatatgata agagtatgtc caaactgttc aaatgtggat gtaaataagc taaaaacttt
     3541 ggttggagaa gaaaatgtaa agactggttg tataggtcag tgtagagcat ttaaaaaaga
     3601 agctgttgga ataatagaca atgagcttga aataaaggaa acagaagcag agttttttga
     3661 agcatgtaaa gaataaattt taaataaaat ataaattata ataaatagag catctaaatt
     3721 gtataag
//
LOCUS       DAMJVX010000257         3598 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.264, whole genome shotgun sequence.
ACCESSION   DAMJVX010000257 DAMJVX010000000
VERSION     DAMJVX010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.264"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(113..964)
                     /gene="gatY"
                     /locus_tag="PC407_003493"
     CDS             complement(113..964)
                     /gene="gatY"
                     /locus_tag="PC407_003493"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2498898.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(1013..2182)
                     /locus_tag="PC407_003494"
     CDS             complement(1013..2182)
                     /locus_tag="PC407_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2498899.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     NRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPQGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            2644..3561
                     /locus_tag="PC407_003495"
     CDS             2644..3561
                     /locus_tag="PC407_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2498900.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     ICSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVNQ
                     GEELIELSKKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKHFSVNTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
BASE COUNT         1223 a          576 c          428 g         1371 t
ORIGIN      
        1 tatttttcat caattaaatg aaaaaaatac accatttaca tataattaat ataaattttt
       61 catataacaa atatcctaaa cttatctaaa ataaatttag gatattttat ttttatactc
      121 tatttacaga cccacacact ttaatctttt cttttacaaa atccttcact gattgaagcc
      181 ctgctaatcc atacttttta gggtctataa cctcagaatc ttcatctaaa acttttttaa
      241 cccctttact atatgcaatt cttaattcag ttgcatagtt aacttttgca atacctcttt
      301 ttatagcttc ttttataata tcatcatgta ttcctgaacc tccatgcaat actaaaggta
      361 ttgaaactac atttttaatt tctgataacc tatctaaatc tattttaggt tcacccttat
      421 acaatccatg tgcagtacct atagcaatag ccaatgaaga tattcctgtt ctatcgacaa
      481 actcttttgc ctcttttgga tttgtataat cattactatt cccgccatct aaatcatctt
      541 cttttccacc aactttccca agttctgctt ccactggtat aagagaagga ctacatgctt
      601 ttactactga tttagatata gatatattat tttcaaagct ttcatgagaa ccatctatca
      661 ttattgatgt atatccactt cttaaagctt tcattgcaag gtcaaaactt gaaccatggt
      721 ccagatgcat tgctacaggt atctttactc tttctgcagc aacctttgca tttgctagat
      781 aataatcaag acctgcatat tttacagtag atggagtagt ttgtaatatt actggagagt
      841 ttaactcctc agctgcttct ataacagcaa ttaccatttc catattttct acattaaatg
      901 ctcctactgc atacccctca tcttgtgcct ttttaagcat tgatgttgtt gttactaatg
      961 ccacattgat accctcccaa attatttagt tataatattt taatcttatc ttttaatatt
     1021 catatattgt tacacctttt actactctat ttactgttcc tgtagctgat ggcgtatctg
     1081 gcttatttcc aacctttacc gatacaaaca atgctatagt ttgtgcaaac aatatgtatg
     1141 gcattgcaag atacacatct ggtaatttat tatacttttc tttaaactca aactttgttc
     1201 ctgagaagtt attttcattt tctactgaca ctgcacaagt gaatttagca attttatccc
     1261 tttttatctc ctctaaaaca tctaaatcat attttcttgt atataaacag ttagatacaa
     1321 attcaaatac aagtgtattt tcatcaataa atgattttgg tccgtgtcta aaccccatag
     1381 gagaatcata caccgtagat atttttcctg ctgtaagttc taaaagtttt agttgtgctt
     1441 cttgtgtcaa accacctaat cctcctgaac ctaaatatac aactctatcg aaatctttat
     1501 ttattaactc atgtatttca tcttttctat ctataacatt tcttcccatt tcaattattg
     1561 catttatata tgacttttct acatcatctt ctaaactatc gaatattaac atagcagata
     1621 acatcataca cgaaaaactt cctgtcatag caaagccttc atcatttgat tttgatggca
     1681 ttaataacaa gtaattattt tcgtcgtttt tagtcatttt agctagttct ccttgcggat
     1741 tacaagttat agctaaatga taaaaattat ctacaatctt attccctaaa tttaaagcag
     1801 ccaaactttc aggactattt ccacttcttg caaatgaaat taataatgtt ggtatatctt
     1861 tttttaagta atcatatggg tttgaaacta tatccgtagt aggtattgct tcaaatatgt
     1921 attttcttat gtcattttta ttcttaaggt aaggaagtat tgaatttcct atgtaagctg
     1981 aagttccagc acctgtaaat ataactctaa actgcccatg attattactt attgtatcta
     2041 tgaatctatt taatttttct ttgttagatt tatatatttc ataagtctct aaccataact
     2101 caggttgttg ttttatttca ttagttgtta ttattgcacc taaatctttt agtttttgtt
     2161 cttctaattt aaacattttc attctccttt catacttgta acgtttacat ttggtaatga
     2221 ggttataacc actttaaact ataaaaaaat atactctttt ataatttttt gcttagattt
     2281 tttagtacta cttaaactca ctataaaata tacgctatag attttaatta attttatagt
     2341 ttatctttaa aaatttaata aaatcatact ttagtatttt tattttacga taaaaacttg
     2401 ttcagaccaa agtggtaatt accactaatc caaatataaa ttaatttgaa ttttttgtca
     2461 atacataaaa tatttatatc aatcaatttt atagatatcc tacaaaatta ctttgtcaat
     2521 atttcttatt tcgttaattt acttatataa gctttaaatt tattttttga tacaaagttt
     2581 acaaatttat gtgtctgaat tataatcaat tctgtaaata taaaaacctt aggagaaagt
     2641 attatgaaaa atataaaaat gggaatgata ggtcttggct ccatagctca aaaggcatat
     2701 ttacctatat taactaagtc tgagagattt gagtttgtag gagcttttac tccaaataaa
     2761 gttaaaaggg aaaaaatctg tagtgattat agaattatgc cattcgattc tattgaatcc
     2821 ctagctaaaa aatgtgattg tatttttcta catagctcta cagaaacaca ctacgaaatt
     2881 ataaaaatat tattaaattt aggtgttcat gtatatgtag acaaacctct tgcttcaaca
     2941 gtaaatcaag gagaagaact tatcgagtta agtaagaaaa aaaacttaaa tttaatggtt
     3001 ggattcaacc gtagattctg tcctatgtac aaagaaatta aaaataatgc tacagaaatc
     3061 gtatctatca atatatgtaa acatggatta aattccttaa gaaatgttag attcgacagt
     3121 acccttatag atgactatat acatgttatt gacactgctt tatggttagc aaatgaagat
     3181 gttgaaatta gcggagaaga tttattttta acagacaata agaatcttat atttgtaagt
     3241 cataaactta aaggtaaaca tttctctgta aacacctcta tgcatagaga ttctggaaca
     3301 aagttagaac aagttgaaat tctttcaaaa ggtaagattc aaagagttaa gaatcttaat
     3361 gtactggaaa tagaagaagg tggaaaccta actttaaaac aaagtggtgc atgggtaaat
     3421 atattaaaac aaaagggttt tgaggatata tcaaatcatt ttattgattg tatagaaaat
     3481 aatataaaac cagctattaa tggagaagag tgtatcaagg ctcaaagatt actagagaaa
     3541 attataaatt cagtaaaata gatatttatt ataaaaatag gttaaaaatg aatttttc
//
LOCUS       DAMJVX010000258         3518 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.265, whole genome shotgun sequence.
ACCESSION   DAMJVX010000258 DAMJVX010000000
VERSION     DAMJVX010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.265"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(147..1163)
                     /locus_tag="PC407_003496"
     CDS             complement(147..1163)
                     /locus_tag="PC407_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2498901.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFVGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDRSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            complement(1346..1708)
                     /gene="spoVAE"
                     /locus_tag="PC407_003497"
     CDS             complement(1346..1708)
                     /gene="spoVAE"
                     /locus_tag="PC407_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HDF2498902.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            complement(1722..2735)
                     /gene="spoVAD"
                     /locus_tag="PC407_003498"
     CDS             complement(1722..2735)
                     /gene="spoVAD"
                     /locus_tag="PC407_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HDF2498903.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            complement(2889..3326)
                     /gene="spoVAC"
                     /locus_tag="PC407_003499"
     CDS             complement(2889..3326)
                     /gene="spoVAC"
                     /locus_tag="PC407_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HDF2498904.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
BASE COUNT         1126 a          639 c          381 g         1372 t
ORIGIN      
        1 tcatattata aaaactatct caataaataa aggagtacaa tgtacttata tacacctact
       61 ccttttaatt tttatttata ttgtttgtta aaaaacttca cataatctat aatattttta
      121 ataaactttt gatacttaaa agcttactac tttattcttt taacgctatt tcgtattatt
      181 aaccttggat ataattttct tggaatgtgt tctgtactct ttttatttat tatatttact
      241 aaactatcta ctccattttt tataacttgc tctcttgggt catctattgt tgataaatgc
      301 gacctaaaat atgatattaa ttcaatatta ttatacccta ttatggaaat atcttctggt
      361 acatttatac cataagtagt ttttaactct tgtattatag aaatcgcagg tacatcttgt
      421 tgtacaaata tagctgaagc tttttttatt ttatcaatat atttttctaa aaattcaact
      481 tgagattcaa aatccatttt agatttaatg acttcggtct ttaaatttgc ctcttccata
      541 gcatttaaat atcccttagt tctatcttca tgcatttttg aactcctatc atctgaagtt
      601 actgttatta tgtctttatg accatggtca attaaatgct tggtagctat ataacctcca
      661 tacacgttat cgtcccaaaa gaaattgtct acttctccta caaaagatgg tttaaaatat
      721 ataaatacat gtggaaaatt attgtctttt aaaaaatcat actcttcttt ttttattgat
      781 tgagacacaa ttactaatcc atcaaccatt tgtccattta ccattttata tacattacta
      841 tcaccactaa tgttattatt aggctgtatt ataaaatcgt atttattagt ctcaatagaa
      901 tttatagaac tatccattat atcactaaaa aactttctag tatcttgatg attaaaatta
      961 tttgaaaaga ttacccctat cctgttagtt tctttttttg ctaaatttct tgcatttaca
     1021 ttaaaatgga accctagtct atttgcctct tctactactt tcttttttgt tttttcagat
     1081 acattaggat tattgtttaa gcacctagaa acagttgaat aactgatacc tagattcctt
     1141 gcaatatctt taatagttac catctttatc ccctacttca cttcatttta ttttgataat
     1201 ataatactac actatgttgt aaataattgt caacttcaat tatgtaaacg cttctttata
     1261 taaaaaatag gtggaatcta aaagatatcc acctgcttta taaaaagtta ttatttcaaa
     1321 aacttattat tcttttaata aagatttatg gttttgcttt tggagtaaat actactgaca
     1381 taatatatcc aaagaatata gctgctgcta ttccaccagc tgtagcagtt gtacctccta
     1441 aaaaaatacc taaaaaacca tcactttgaa ttgattttat tactcctgta gcaagtgaat
     1501 atccaaatcc tattatagga actgttgcac ctgctgctgc aaacttaact aatggctcat
     1561 aaagtcctag aaaagttaaa actacaccag ttgttacata cgttaccata acatatggag
     1621 tttgcatttt gaaataatcc atcattactt gggctattaa acatataatt ccacctacaa
     1681 taaatactct tacatagtcc attaacatat tatatcactt cctactcatt tactattact
     1741 actgcatgtg caacacatgg tattgtctgc ttttgtagtg tacttgtcgg tgaaagtagt
     1801 gctcctgttg atacaagcat tactttgtta aattcttttt ttctcaactt atcatatata
     1861 tatcctgcaa acactgtagc tgaacaccca catccacttc caccacagtg gacatcttgt
     1921 tctttaaggt taaacatctc tataccacag tcagtataaa cttttgatat atctacacct
     1981 ttttctttta gaaagtcttc tgctatctgt tttccaagag tacctaaatc tccagtagca
     2041 attatatcaa agctatttgg gtcatcatta gtatcttcaa agtaagagta gatagtatct
     2101 attgctgctg gagccatagc tggtcccata ttattaccat catcaatacc ctcatctatt
     2161 actttaccaa cagtaacata ctttactttt ggaccgtctc cattaggagc taataataca
     2221 cttcctgctc ctgtaactgt ccactgagct gtcattggtt tttgatttcc aagttcaagt
     2281 gggaatctaa attgtctttc tgctgtacaa tagtgacttg atgtacctga cagtactaaa
     2341 tcagcaaaac caccatctat aagcatagaa cctatacaaa gaccttctgc cattgtagaa
     2401 caagctccat atatacctaa aaatggaata gccagttctc ttgctgcaaa tgatgctggc
     2461 actatttgat ttattaaatc gccacctagc atataattta catctgacaa ttttttacct
     2521 gccttttgga tagcttgttg ggatgcagtc tctaccattt tactttcagc taattcccaa
     2581 gtcttttcac catataaatc gtcagtcata atcatatcaa aatagtcctt tagtggtcct
     2641 tcaccttcct taggacccac tatagttcca gttgatatta tggttggttt attttctaac
     2701 ttgactgttc tttttccaat tcttttattt ttcatatttt cacacctttt taattttgca
     2761 ttttactatt caaattttta atattaatta aatctgcaca attatttttc aattagttac
     2821 tttagtagct attttcagat tttatacctt cttcataatt aataaaattt aaattaaata
     2881 aaaatcatct aaaacatctt aaaaatataa taaataattc cacataaaat agaagaacct
     2941 ataccataaa ctaaaactgg tcctgcaatt ttaaataagt ttgcacctac acctaataca
     3001 tagccttctc ttttatactc cattgctgga gatactattg agttagcaaa acctgttatt
     3061 ggaattatag aaccagctcc agctcttttt cctattaggt cgtaaactcc taatccagtt
     3121 aaaaatgccc ctataaatat taatgttatt gatgttgcag aactagcacc tagtttatca
     3181 agacctccaa ctttcatata caaatcattt attgcttgcc ctatcataca aataattcca
     3241 cctacaataa atgcaagtgt atagtttttt aaatatgtag gttttgggct tatttggtct
     3301 acatattttt tataattttt atccatacta aacacctctc taaagtatta tttgttaatt
     3361 ttaaaaaaat aaacctatta tttattttga ctatctaaag tttggtcaaa aaataaaaaa
     3421 gatgcctaat aaagaaaaaa tctatttttc cataaagaca ccttgttttt atatttttac
     3481 ttttttaatg ctttttatta ttttagtatc ttttaata
//
LOCUS       DAMJVX010000259         3460 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.266, whole genome shotgun sequence.
ACCESSION   DAMJVX010000259 DAMJVX010000000
VERSION     DAMJVX010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.266"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            46..381
                     /locus_tag="PC407_003500"
     CDS             46..381
                     /locus_tag="PC407_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2498905.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            518..1795
                     /locus_tag="PC407_003501"
     CDS             518..1795
                     /locus_tag="PC407_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDF2498906.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNNSQKDSNNDNNDNG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            1916..3304
                     /locus_tag="PC407_003502"
     CDS             1916..3304
                     /locus_tag="PC407_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2498907.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSESAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
BASE COUNT         1422 a          339 c          603 g         1096 t
ORIGIN      
        1 taaaagtttg tttaataaat taaataaaga aaagaggaga ataaaatgta caacatagat
       61 gatatgatat ataattgtcc agtagaagct ttatcaaata ttttaggtaa aaaatgggtg
      121 gctataatta tatggaaaat acaagaagat aagaaacgat ttggagagct acaaagagct
      181 ataccagact gtagtaagaa aatgttagtt caacaactag attttttaat tgaacagggg
      241 attatagtca accaaaaaaa gttggttaat aatgttgtag agtcaacata ttttttaagt
      301 gaatcaggat tacgattgct tcctgtcatg gaaaaaatga ttatgtggag caataaaaat
      361 ttaaattgtg ataaatacta aaatttactc tgtaagtaat gaaaatttaa ttaagctatg
      421 agtagaaaaa tgtattaaaa ttacataatt ttttagttag attttaattt agaaatggga
      481 ttgtaatatt ggaaataaaa taaggaggaa ttttaatttg caaaatgtaa atttattttt
      541 agtatttata gaaggtatag tatcattctt ttcaccatgt atactgccaa ttttgcctat
      601 ttatttgagt atattgtcaa atagtagtgt agaaaatcta aaagaaggaa agacgagttt
      661 tataggaagt tctttattta aaaatactat attttttgct cttggaatct caacaacatt
      721 ctttatattg ggttcatcag taaaagtatt gagtatgttt tttaatgaaa ataaagattt
      781 aataatgttt attggtggga ttataattat tatcatggga ttattttaca tgggaataat
      841 taaatcttca atattgaata gagaaaaaag atttaatgtt aagtttaaag aaatgaaagc
      901 aataacagct tttatattag gttttacgtt tagttttgga tggacacctt gtattggtcc
      961 aatattggca tctgtattag tcatggtttc aagttcaagt aatcatctta gtgccaatct
     1021 tttaatagca gtatatacta ttggttttat tttgccattt ataattacag ctatgtttta
     1081 cagtaaacta tttaaaacta tagataagat aaaatctaac atggaaatta ttaaaaaaat
     1141 aggtgggatt atacttatag tatcaggaat attaatgatg gtaaatggat ttggaagtat
     1201 aagcaaacac tttaatacgt cacaaaatag caaaattgaa agcaagcaag aagaaaataa
     1261 gcgagaaaat tctactgata aagaagaaaa ttctgatggt aataatagtc aaaaagactc
     1321 taataatgac aataatgata acgggtctaa tgatgaagat agaataaagt ctattgattt
     1381 tactttaaca gaccagtatg gtaaaacaca taaattaagt gattatgaag gcaaagttgt
     1441 attcttaaat ttctgggcta catggtgtcc tccatgtaaa gaagaaatgc cttatataga
     1501 acaactgtat aaagactata ataaaaacaa tgatgatgta gtaattttag gagtagcttc
     1561 acctaattta ggtagagaag gttcaaggga acatgtagtt aacttcttaa aagaccaagg
     1621 ttatacattc cctgttgtac ttgatgaaga tggagcatta gcatatcaat atggaattaa
     1681 tgcttttcca actacattta taattgacaa ggaaggatat gtaactcagt atattccagg
     1741 agctatggat aaggcaacta tggcatcatt tattgaaaat cagagaaata aataaaagta
     1801 tttaagtata tgtttatgaa tcaatttaag gttacaaaaa agtgcctaat tgtaccacct
     1861 aaataatctt gttatagtaa aagtataaac aatacaagaa agggttgaat tttatatgaa
     1921 aaaaacattt gatgctataa taataggatt tggaaaaggt ggaaaaacat tagcaggaga
     1981 tttggcaaac aggggattaa aagtagcttt aatagaaaag tcaaacaaga tgtatggtgg
     2041 gacatgtgta aatgtggcat gtataccaac aaaaagctta gaaaattcag caaacagcgt
     2101 aaaaacaaag aatataaatt cttgggatga agtacaagct gaatatgaga aagctataga
     2161 taaaaaagaa actttaatta caaaactcag agaagctaac tataataaat taaattcaaa
     2221 tgagaatgta actatattca caggaatggg aactttcatt gatgaaaaaa cagttcaagt
     2281 taaaactgaa aatgaaattt atgagttagt agcagataat atatttatta atactggttc
     2341 aagaccattt atacctaata ttaagggaat tgaaaataag aatatagtat atgatagtga
     2401 gtctcttatg aatctaagag ttttacctaa aaaaatgaca attataggag ctggatttat
     2461 aggtttagag tttgctggta tttatagttc atttggagca gaagttacaa tattaaactc
     2521 taataatggc attttaccaa atgaagatgt tgaggattca gaagaaataa taaaattact
     2581 tgcaaagaga aatgtaaaaa ttgttaataa tgcaaatata aaagagatta aagaagtttc
     2641 tgaatcagca atagtagaat atgaagtaga tggtaaatct aaagaattaa caagtaatat
     2701 gatacttgta gctacaggaa gaaaagcaaa cactgaaggg ctagggctag aaaatgcagg
     2761 tatagaatta aatgaaagag gatttattaa agtctcagaa actcttaaga ctaataaaga
     2821 gcatatatgg gctataggag atataaatgg aggtccacaa tttacttata tatcattgga
     2881 tgattatcgc atagttatta atcagttatt tggagataag actagaacaa ctaatgatag
     2941 aaagaatatc ccaaattcta tatttataag tccagcattt tcaagagttg gtcttaatgt
     3001 taaacaagct aaagaaaagg gatatgaagt attagtggca aagatgccag tagaagcaat
     3061 tccaagagct aaacagattg gcaaggcaga tgggtttata aaaatagtga tagataaaaa
     3121 atccaataag atacttggag ctagtatgat atgtgaaaat tcaagtgaga ttatccatct
     3181 tattcaatta gctgtagatt tagaagtaga gtatacatat ttaagagacc gtgtttatgc
     3241 acatccaact atgacagaag ctcttaatga tattctatct cctaatatga taaaagaagt
     3301 ataacataag tttaatatat aaacctaaaa ttatcattat aagtagattt tagaaagagg
     3361 ctatctcaaa aaatagagat aagtctcttt tttacttttt aaatgataaa aatatatttg
     3421 attttataaa agaagattat cttatgaact aaaaagttta
//
LOCUS       DAMJVX010000260         3454 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.267, whole genome shotgun sequence.
ACCESSION   DAMJVX010000260 DAMJVX010000000
VERSION     DAMJVX010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.267"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            8..1081
                     /locus_tag="PC407_003503"
     CDS             8..1081
                     /locus_tag="PC407_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2498908.1"
                     /translation="MNKFKLKRSILISLIILIFIFIVYFVSIELKDGSLVNNQYKGKI
                     KSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIKLIKK
                     LNKKGISTILEAYPWVENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPLDVNV
                     LNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDSSSKLFYEQKLNN
                     NFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKAHRKP
                     IFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSVFCVG
                     KIDDEKNFYPSKESIKVIKSWYE"
     gene            1135..2259
                     /gene="wecB"
                     /locus_tag="PC407_003504"
     CDS             1135..2259
                     /gene="wecB"
                     /locus_tag="PC407_003504"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDF2498909.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKALVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDSYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            2337..3311
                     /locus_tag="PC407_003505"
     CDS             2337..3311
                     /locus_tag="PC407_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2498910.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSVTIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDIIRSYNLQLIDNKVETK"
BASE COUNT         1444 a          337 c          605 g         1068 t
ORIGIN      
        1 gaaatatatg aataaattta aattaaaaag aagcatttta atctcattaa taatcttaat
       61 ttttatattt atagtgtact ttgtaagtat tgagttaaaa gatgggagtc tagtaaataa
      121 tcaatataaa ggtaagatta aatcggccaa tttatctgta gattatgaaa taaatcaagt
      181 tatgaaggat atagataaat tgggattgaa tacagtaaat gtacctataa ttataaatgt
      241 agagtccatt aattctgata ttatgtcaat agataataat agtaaagaaa aggcaattaa
      301 gttaataaaa aaattaaata aaaaaggtat ttcaacaata ttagaggcat atccatgggt
      361 tgaaaatgga aaattgtatg agactgactg gaaccctata aataaaaaaa gattttttta
      421 tacttggcaa aatgccatat taaatgaact tataattgat gtagcaaatc cactggatgt
      481 aaatgtttta aatattggtt ctaattttgt acatttggaa gaatatcaac aaaattgggg
      541 tgaaataata gattttgtac aatccaaatt tgatggtctt gtaacgtaca gaactaattg
      601 gtggtataca aaaaaagatg attcaagtag taagttgttt tatgaacaaa aattgaacaa
      661 taattttttt gataagttag attttatatc tatagcagca tattttgagt taagtaataa
      721 acctgttaat acagtggatg aattaatttc tgccttacac tcatctactg taaacaatag
      781 aggacaaaat ataaaacaag aaatatataa cctttataaa gctcatagaa aacctatatt
      841 ttttggagaa ttaggttttt caaatagaga aagtgcaagt tctcagccgt ggaatcatac
      901 tccttcaaaa gctgtaaatg gcgaagaaca agcaaggtgt tttgaggcat ataagaaagt
      961 ttttgaaaat gaagactgga taaatggttt ttctgtgttt tgtgtaggta aaatagatga
     1021 cgaaaaaaat ttttacccat caaaagagag tataaaagtt attaagtctt ggtatgaata
     1081 attttaaaag tgtagaattt atatagatta aatagatgat taggagagat ataaatgaat
     1141 gacatcaagg ttatgactgt atttggaact agacctgaag ctatcaaagt agcaccttta
     1201 ataaaggaac ttgaaaaaag agagaatata aaaagtattg tttgcgttac agctcaacat
     1261 agagaaatgc ttgaccaagt tatagaaacc ttcaatataa atgtagacta tgatttggat
     1321 ataatggaaa aaggacaaag cctgaatgat ataacatgta agattttaaa taaacttccc
     1381 ttgattttaa acaaggaaaa tccaaatata atattggtac atggagatac aaccacaact
     1441 ctagctacaa gtttgactgc attttataat aaagctcttg ttggtcatat agaagctggg
     1501 cttagaacct atgataaata ttctcctttt ccagaggagt taaatagaca acttacaggt
     1561 attattgctg atatgcactt tgctcctact aatttggcta gaaaaaactt gatttctgaa
     1621 ggaaaaccta ataataatat atttgtaact ggtaatactg ctattgatgc tttaaagatg
     1681 acaataaaag aaaactataa tcatcctata attgatgaga ttggtaacga taggatgatt
     1741 ttgttaacct ctcacagaag agaaaatttg ggtaaaccta tgaaaaatat attcagagct
     1801 ataaagcgaa tagtagatga ttttgaagat gtacagatag tatatcccat acatttaaat
     1861 cctaaaatac gaactatagc tgatgagata tttggaaaat ttcctgaaaa aattcatatt
     1921 atagagcctt tagatgtagc tgattttcat aactttttaa ataaatctta tatgataatg
     1981 actgatagtg gaggaataca ggaagaagct ccatcattag gtaagccagt ccttgtgttg
     2041 cgtgataaaa cagaaagaac agaaggaatt gaagcaaaga cacttaaatt agtaggtact
     2101 aatgaagata gaatatataa ttcagtgagc gatttattaa taaataaaga tagctatgtt
     2161 cagatgagta aagcatctaa tccatatgga gatggaaatg caagcaaata tatagtggat
     2221 ataataatta aaaaatttaa ttgcaaatat ttaaattaga taaggtctaa aacattaact
     2281 aattgatata agatttatga taatattaag gaatacaatt tttaaggggg agtaatatgg
     2341 ctagaaaatt aataaaaaat ttaggtaaaa gtaaaagtgt aaagagagtg aaacttttat
     2401 ttaagaagat ttttattact gtattcatag tagcaagtat agttgctatt tttaatataa
     2461 cgaaatattt tgaggaatta tacaaagtaa gggatttaaa aagtactaaa attgaatact
     2521 atatggatgt ggctgatgaa gcaggagatg gaaaggtcca attaagttgg aaggccttac
     2581 ttgctataga catggtaata catgatgaag atttaagtaa tataaaaaag aaagacacat
     2641 tggatatagg ggaaaagttt atagtagaag ataaaaatga taaaggcgaa aaggtgtata
     2701 aggtaaaaaa gtttaataag gtattaagcg aattgaaatt tgactcttct caaaaaagta
     2761 gagcaagaaa atacatgaaa gatttggaat acacatacct tggaaataaa caattagata
     2821 gtagtgatga aaaaattaaa tttataaaga agttagaaga ctcagctata agagaatata
     2881 ttgattatgg aatattgccc tctgtaacaa ttggacaagc tatattagaa tctggctggg
     2941 gaaattctaa acttacaaaa cagagtaata atttatttgg tataaaagca gataaagcat
     3001 ggaaaggaaa aagtgtagaa atttcaactt cagagcatta taatgaaaaa attgtagcta
     3061 gttttaggtc gtataattca ttacaagatt ctgtcaaaga ccacagttta tttttaatta
     3121 ataataaaag atatagaaaa catgggttgt ttgaggcaaa agattatatt agtcaagctc
     3181 aagcattaga aaatgcagga tatagtacag cagaagataa aaaaggaaat cgtatatatg
     3241 cagagctatt aatagatatt attaggagtt ataatttgca attgatagac aataaagttg
     3301 agacaaaata attgttaact ttatatgagg agataacaag ataagattct tttctacaaa
     3361 aagaatagaa aaattgtaaa aaaaactaaa aaaatgtatt gacttgttat ttaaaaggtg
     3421 atattattat tactgtactc gaaagagaac aaaa
//
LOCUS       DAMJVX010000261         3402 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.268, whole genome shotgun sequence.
ACCESSION   DAMJVX010000261 DAMJVX010000000
VERSION     DAMJVX010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.268"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(16..2097)
                     /locus_tag="PC407_003506"
     CDS             complement(16..2097)
                     /locus_tag="PC407_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDF2498911.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASSFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGTNINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKPKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYIKKYKRR"
     gene            complement(2120..3376)
                     /locus_tag="PC407_003507"
     CDS             complement(2120..3376)
                     /locus_tag="PC407_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2498912.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYKVSKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIEGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTTVIISDILCVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
BASE COUNT         1140 a          541 c          312 g         1409 t
ORIGIN      
        1 aataaattta tcatattatc ttcttttata tttttttata tatagcattg ataatatcat
       61 gactacaatt atagtaataa aagaaatcat taaaaatact ttagttgtct tatttatact
      121 catattccag cttgaatctt ttacttcttt cttaggtttt aaattataag ctaaatcttc
      181 tacctctccg tattcatcaa ttatcaatgt atctcctttt aaatcattaa ctttactttt
      241 attcaacaaa taatccattc ctaaatataa gtttttttca ttcattgaac taattaccat
      301 gatatttttt tgattattat atggtgattt tattaattga atggtagaaa tattttttcc
      361 ataatcatca ataaagctta tcttatcatt tgatataaaa ttggagtaat ttttatcgaa
      421 ctttatattt aaattattat ttaacatctt tataacagaa ttattatgtg gcactccaaa
      481 aactacaata ttagtatcct tatacttgtc actaaattct tttcctctaa ttacatttat
      541 atttccatta tgtgaattta tattcgtacc taaagtaaca cctaatctaa acatccaggt
      601 catagcttgt gaccctgaat aatctggcat tatcacagtt aaatcattaa actcatcatc
      661 tctaacaaat ggatacggat aattttcaaa agataaggtt tcattttctt tcgtagataa
      721 cgccaaataa gaattatttg atacatatgc ccaaggatta ttactctctc tagtaacaca
      781 attcgaattt tttatactca aattaaatgt caatttaact tgataatagt ttttattatc
      841 tatatcttta ggtattttta attctaactt atcattatta gaatgtgacc tatccaattt
      901 tttacttccc acaataacat catttataga cactgtaact aaagattttt caaaatctaa
      961 attgtcagaa tatcttaaat ttaagataat ctttgaacca tcatctaata ccttacccgt
     1021 tggtattttg acatcaaaca aagcttgttg attaaatgct ccttccaaca aaaagtcaga
     1081 atatcctaag tcttttaatg ttaaatgacc taaatttaat ttttgttccc tattaatttt
     1141 tatattagtc tctttgttta caagcactga tgatgataaa acttggttag aaagtctatt
     1201 ttctattaat aaatttgatg cctttattaa atcatcttca ttactaccaa ttaccaccat
     1261 catcttctta ttcttattgt atggtgaatc tacttgcttg attatacagt ttgaagataa
     1321 tagggtttgt tcctttatac ttaaaatatc caaaatttct tctgctgtat tttcaggttt
     1381 accaatatat ataatattat catctgacca atttttcatt tctgaatata atttaacatc
     1441 taactttaaa tcatcatttt ttgttatttt tccaaatgat gatgccaaat taaacactgc
     1501 tgtagtctct cctctagtca tattatctgg tactactata gttgtatcta atttatgatt
     1561 attttcaatt tctgcatatg gatacggata ttcatttatt gagttttcca cctttttctg
     1621 cttatatcta attgatatat atgattcttt gtgaataacc atccagttcc cagtattgga
     1681 atcatcttgg caaattttat cagatattgt tttgtatgtt tttattttta tttcattata
     1741 tccttgaatt atataatctt ttggaactaa tacttctaat gtatttttat aatttgtctt
     1801 agcatttaat ttaatagatt ttattggtac attatttatc aatacagtta aacttgaaac
     1861 atctccattt aatatttggc tcttactaaa attaagatgc aataaaactt cttcaatatc
     1921 ccaattttta tttacttcaa aaaaagtact attacttcct ataacaccat caatagttat
     1981 atctctttca aacttataat ttttaacttt agtctcatca gcatttactt tgtatataag
     2041 tgatatattt gaaaaaaaca atactaaaga tataattgat ataataaatt ttttcatgtt
     2101 aattcctcct gtacatgaat caaaatcttt ctgttttata ccatttaacc tctcttttaa
     2161 aaattatatc ttttagatac tctatcatcc cttttatagc tacaaacagc catagttgag
     2221 agtatgtaaa atacattatt gcaactatga atatattttc aattgttgct tcaccttttt
     2281 ctatagtaag gcttatactc acttctataa taaataaaag ataagatagt atccatatta
     2341 gaaaaaagtt aattggaata gaaatttcta ctagtttaga tatacttaaa acacataaaa
     2401 tatcggatat aattacagtt gttaaaaata gaaaatatac agaaaaaaag taagctatat
     2461 caaacacaat cttatttctt ccctgtttaa atatattttt tatatatttc attaatacat
     2521 atatattccc ttttgcccat ctagtacgtt gctttatcca caccttaaca gtttctggct
     2581 cttgttccca tgttatagat tgtggtacaa gctttatctt gtatcccaat ttatatatcc
     2641 taaaacttat ttctgtatct tctgcaattg ctttactatc ccatccttca atttcttcta
     2701 tgatacttct cctcaatata aagttagttc ctggtattgt acatagatta aaaagttgcc
     2761 atctaccagc ttgtgacatc cattgaaagc ttaatgtttc tatgtttata aatttggtta
     2821 ataggttttt atttttattt cgagtcctaa atttcccaat aacagcacct aattcatcat
     2881 tcattactat agtttgtact agatatctaa gtgcattttt atctggtgtg ttatctgcat
     2941 cataaacagc tataaaatct ccttttgaaa ccttatatcc aatatttaat gcattagact
     3001 ttccttttcc acctgttaaa ctatctgtat ttattatagt gaaattataa ttgttgtacc
     3061 tatcctttat attttctaaa atttctttac tattatcaga agaattgtca tttataacaa
     3121 taagttccat cttactctta ggataattga gtaataacaa agactccaca gttctaccta
     3181 tcactttagc ttcattatga gcaggaacaa gtattgatat cataggatat tcatcaattt
     3241 cttttatttc tttatcaaag tttttaaaat aaaatatata tccacccata gctaaaataa
     3301 tatttataag caataaagac catatactaa acagactaaa tacgaacaaa tactctgata
     3361 catttaaatc tctcataaat ttctcctaaa atattttaaa at
//
LOCUS       DAMJVX010000262         3395 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.269, whole genome shotgun sequence.
ACCESSION   DAMJVX010000262 DAMJVX010000000
VERSION     DAMJVX010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.269"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            97..1695
                     /locus_tag="PC407_003508"
     CDS             97..1695
                     /locus_tag="PC407_003508"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HDF2498913.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFDVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1930..2121)
                     /locus_tag="PC407_003509"
     CDS             complement(1930..2121)
                     /locus_tag="PC407_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HDF2498914.1"
                     /translation="MAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQ
                     SAGTAATLTIFRIADTVMT"
     gene            complement(2290..2883)
                     /locus_tag="PC407_003510"
     CDS             complement(2290..2883)
                     /locus_tag="PC407_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HDF2498915.1"
                     /translation="MRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYY
                     NLRTITWNDRVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNY
                     PYQIFEFTSSRDCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSIGPMG
                     ATGPTGATGNSSQPIANFLVNAPSPQTLNNGNAITGW"
     gene            complement(2884..3030)
                     /locus_tag="PC407_003511"
     CDS             complement(2884..3030)
                     /locus_tag="PC407_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HDF2498916.1"
                     /translation="MRKIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFF"
BASE COUNT         1229 a          475 c          459 g         1232 t
ORIGIN      
        1 ctaaattttt ttctttttac aacaaatact cactaccttt tgatataata tttttatcaa
       61 ttattattat attcaagttt tgaaaggagt attaaaatga gtttattagt tttcggacat
      121 aaaaatccag atacagattc aatatgttct gcaatttcat tagcacattt aaagaacaaa
      181 ctaggaataa aagcaacagc ttatgcttta ggagatatca gaaaagaagc taaatatgcc
      241 ttggattact ttaaggtaga tgcacctgaa attttagata atgttagaat acaagttaga
      301 gatttaaatt atgataaagt agtaccttta acaccaactt catctatatt agaggcttat
      361 aaccttatgg atgaaaaaaa tgttaaaaca ctacctgtaa cttatgatga tggaactttt
      421 gctggtatca ttactatgaa agaaatagct aaaaatctac ttcatcaaca ctttacaact
      481 atacacacat cactatcaaa catttgtaaa aaccttaatg gtactattct tgtggactgt
      541 ggtgaagata taaaaggtag agtagtcact ttatcatttg gaatggagac tctaatagag
      601 actttaaaag aaggagacat agctattgtt ggagatagat atgatagtat tgaatatgct
      661 attgatacaa aagttaaact tttaatactt actaatcata cagagatttc aaaagatttg
      721 ctagctcttg ctaagataaa tggagtgtct gttgtttctg ttgaaagtga caattataaa
      781 acttctaatg taataaatca atgtagtttc ttagactcta tagaagctac agaaaattta
      841 attacattca aagaagatga ttatatggat gaaataaaag agattatgct agaaacaaac
      901 ttcagatctt atccaatatt agatgatgat aataagtttt taggtttagt ttcaaaaggt
      961 catctattaa acccatctaa aaagaatgtt gttttagttg accataacga atatgctcaa
     1021 agtgctgatg gaattgaaca agcaaacata gttgaaataa ttgaccatca taaacttggt
     1081 ggtatttcta ctgatgttcc tatgtcattt agagttatgc ctgttggatg caatagtact
     1141 ataatttatc aaatgtacaa agaaaacaat gttgagatac cttatgaaat agctgggctt
     1201 cttttatctg ctatattatc agatacatta ttatttaagt ctccaacaac aactgatatg
     1261 gataagaaag cttgtgaaga attaagtaaa atagcaaaag tagacatgga aaaatacgct
     1321 atggatatgt ttaaatgcgg tacttcttta gatgaatata ctatagaaga aatagtaaat
     1381 atggatttta aagaatttaa tatgagtgga catagagttg gtattggaca agtatttacc
     1441 ttagacatag actctatatt tgctaaaaaa gatgagtttt tatcttatat aaattcgact
     1501 gattatgaca aactaattct tgcggttaca gatataatca aagaaggttc ttatttaatc
     1561 tataaagcag aagatagctt aattgctaac gcatttgatg ttgaaggtgt tcaaggtaca
     1621 tttgcaccag gtgttgtatc aagaaaaaaa caattagtcc ctaatttaac tactgcaatc
     1681 aaaaatttta aataatataa aatttttaaa taacgaaaac tgtctcaaat ctatatttga
     1741 gacagttttt tattatacat atattgataa taaagttaaa taaaaatcca taaatttaaa
     1801 atttttatga atatatttta ttattattgt tattttattc attctaaaat aggtaaaact
     1861 caatatataa aaatactttt gtcatttaaa taaaacctaa atagttttta agattatttt
     1921 agacacgttt taagtcataa cagtatcagc tattctaaat atcgtcaaag tagccgcagt
     1981 tcctgctgat tgcccatttc gtatccctac tgttgtagat gaaaaattat ttattgtaaa
     2041 agtagtcccc ccaaataaaa aaccagcaaa atatccagaa agtactcctc cgcctcctgg
     2101 ctcagtagta agccctgcca aatcttttcc tcctaaaatt gggaatagga tagcaaaaga
     2161 atattgattt atacttgatg aacctggttg taatgctact gaaactgata tataatacac
     2221 accattttct tgtactgtaa acgtaccatt tgcatctact gttatacttg aactatttcc
     2281 tattattgtt taccaacctg ttatagcatt tccattattt agtgtttgtg gagatggtgc
     2341 atttacgagg aagttagcaa ttggctgaga ggaattgcct gttgctcctg ttggacctgt
     2401 tgctcccatt ggtcctatac ttcctgtcgc tcctcttggt cccattggac ctgtaattct
     2461 aattggaaga cactcacatt taggataaca attatctata attctatctt caaatgttac
     2521 aagtatatac ggtttgttac aatccctact agatgtaaat tcaaaaatct gataagggta
     2581 atttaaagat aaagctaacc cataattagg atatttattc attgcccatg cttttatagt
     2641 tcccgtaata tttaaacaaa tatagttgga tgtatcactt atcccaattg gtaaatatcc
     2701 cctgatattt tccatagaca ctctatcatt ccatgttata gttcgtaaat tataatattc
     2761 acttattctt ttaatttcaa tatctacttt ttctttaaaa atatgattct tatttgaatc
     2821 aatatgaata aataactctg cttttaatat acttttgtct tttaatatat ctggtattct
     2881 cacttaaaaa aaagtaatca acttttcttt tacaaaacta tttgaacatt tacttcctat
     2941 taagcaataa tctaaattac taaagttttt atctggatat tttttagata taaaagtatc
     3001 atcatttaaa taaagtataa tttttctcat ttataatccc tccattttgg gtctatatca
     3061 tactatgttt aaaatacatg cttagttaca atacaattaa aatttatagt attaaatttt
     3121 tatataataa tgctgatttc tacaaaaaaa gaacatgtca ttagaaactt ctaatagcat
     3181 gttctttttc attattatat attacacttt aaattattcc ttgactggct tagctggtgt
     3241 aaactaaacc agtctttcta aaatattaag cacttttttc ttattatgtt ttttaggatt
     3301 tattgtctct attattgtaa accaaagtta taaaattgta aatagaatat attaccataa
     3361 caatattttt ttataagtaa aaattataaa aaacc
//
LOCUS       DAMJVX010000263         3343 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.270, whole genome shotgun sequence.
ACCESSION   DAMJVX010000263 DAMJVX010000000
VERSION     DAMJVX010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.270"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            195..1448
                     /locus_tag="PC407_003512"
     CDS             195..1448
                     /locus_tag="PC407_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDF2498917.1"
                     /translation="MLNKKNISSIIMTMILSLSTTVFVFADENTNVKGQDVTKYNEEM
                     VSKNLEDLSIEQLIDIRDKAIDKNLEGLRKFGEDSVKERKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVEYENSINDIMDPSGDFFKVTNQSYQINEFVLKNGKNYKEWSY
                     EYVEFLIEHLEDLCNFVDFDAKYVIEMVDPSIKPDLSDNQKKSLNNELIKMYSSNTLS
                     TNIKDEIENWENNLNINEKNSNEPLPRVSPSSYRQQALAYAKKHGYTNGYYGSTNRKN
                     NAPSPYYNFQKDGYGDCANFVSQCLHEAGVTFWTNNNPWYYYSTSNRSPSWAGAREFK
                     IHWMNRIAYQTLTVSNSLSFLRPATPVSILNSSGVATHTLISTDKHSNGYNFSYAAHS
                     DEGARTNLLKKLEGKKISYYKVYWE"
     gene            1782..2513
                     /locus_tag="PC407_003513"
     CDS             1782..2513
                     /locus_tag="PC407_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498918.1"
                     /translation="MLNKKNVSLIVLVIVLSLFTAIFVFNNRNTNDKSKEIAKNNEEI
                     VPKNLEALSIEQLTKIRDKAIDKNLEGLREFGEDSVKQRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQSYEINEFMLKNKKNYKEWSY
                     EYIEFLIDHLDELCKFVDFDIKDVIDIIDPTIKTDLSEEQKKSLNDKLKKVSSSDTLS
                     EDIKKEIKNWENNLNSLNMNKNNSN"
     gene            2755..>3343
                     /locus_tag="PC407_003514"
     CDS             2755..>3343
                     /locus_tag="PC407_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDF2498919.1"
                     /translation="MFNKNEILKKLIEEYDVKTTTDIQDMLKDLFASTIHQMLEAELD
                     DHLGYDRYGNKNKNTKNSRNGYRNKNVKSDFGEITLNIPRDRNGDFEPKVISNYKNDI
                     SGIENQVIGMYSKGMSTRDISSHLKSIYGIDISHSLVAKITDRVLPLAHEWQNRPLDM
                     VYPIVFMDAIHYKVRSEGRIINKAAYIAIGVNLEGI"
BASE COUNT         1420 a          323 c          497 g         1103 t
ORIGIN      
        1 ttatatataa gtgtctaaaa aacgattctt tattcgttat atatataaat aatactagaa
       61 agggaggatt atattatgat atttagtaaa cgctctttta gaaaaaaacg gtgataaact
      121 tgttttaaat tttagtagaa tatgattttt gttttggcaa gtattaatac atatattaaa
      181 gagggaggtt taatatgtta aataaaaaaa atataagttc aattattatg acaatgattt
      241 taagcttatc aactactgta tttgttttcg ctgatgaaaa tactaatgtg aaaggacaag
      301 atgtaaccaa atataatgaa gaaatggtct ctaaaaactt agaagattta agtatagaac
      361 aattaataga tattagagat aaagctattg ataaaaattt agaaggtctt agaaaatttg
      421 gagaagattc agtgaaagaa cgtaaagatt caattttaaa ctttgaattt ggattagtag
      481 cttttgttaa caaaaattat agtaacttaa cagaaaaaga gaaattaata agacaaatca
      541 atattgttga gtatgaaaat tctataaatg atataatgga tccttcaggc gattttttca
      601 aagtaacaaa ccaaagttat cagattaatg aatttgtatt aaaaaatgga aaaaactata
      661 aagagtggtc gtatgaatat gtagagtttt tgattgagca tttagaggat ttatgtaatt
      721 ttgtagattt tgatgcaaaa tatgtaatag aaatggttga tccttcaata aaaccagatc
      781 tttcagataa tcaaaagaaa tctctaaata atgaattaat aaaaatgtat tcaagtaata
      841 ctctaagtac aaatataaaa gatgaaatag aaaactggga aaataattta aatattaatg
      901 agaaaaattc aaatgaacct ttaccaagag taagcccttc tagttataga caacaagcat
      961 tagcttatgc taaaaaacat ggctatacta atggttatta tggtagtaca aatagaaaaa
     1021 ataatgcacc ttctccatat tataattttc aaaaagatgg atatggagat tgtgctaact
     1081 ttgtttcaca atgcttacat gaagctggag taacattctg gacgaataat aatccatggt
     1141 attattattc tactagtaat agatcacctt cttgggctgg agcaagagaa tttaaaatac
     1201 attggatgaa tcgaattgca tatcaaacac ttactgtatc aaactcttta agttttttaa
     1261 gaccagcaac acctgtaagt atattaaata gtagtggagt agcaactcat acactaatat
     1321 ctacagataa gcattcaaat ggatataatt tttcatatgc tgcacattca gatgaaggtg
     1381 caagaacaaa tttgttaaaa aaacttgaag gaaagaaaat ttcttattac aaagtttatt
     1441 gggaataaat aaaaatttat acttttaatt aaatgaaaat tgaaaagaat aaatatctat
     1501 ttcaagatcc aataagtatt tagttgaagc agctattaaa ttttattaca taatcataaa
     1561 aaagagacta gtggaattta gtctcttttt tatgatttaa aaatttaata aaagaattat
     1621 ataaatttgt ctaaaaatta gtttatcatt cgttataatt atataaacta tatcaaaaaa
     1681 gtgttctatt gtattcttta gaataatcag taataaaggt tattctaata aactgagtta
     1741 ttttttcaca aataaaatac ttattaaaaa ggagatttga tatgttaaac aaaaaaaatg
     1801 taagtttaat tgttttagta atagttttaa gtttattcac tgctatattt gttttcaata
     1861 atagaaatac aaatgataaa tcaaaagaaa ttgctaaaaa taatgaagaa atagtgccta
     1921 aaaacttaga agctttaagt atagaacaat taacaaaaat tagagataag gctattgata
     1981 aaaatttaga aggtcttaga gaatttggag aagattcagt aaaacagcgt aaagattcaa
     2041 ttttaaattt tgaatttgga ttagttgctt ttgttaacaa aaattatagt aacttaacag
     2101 aaaaagagaa attaataaga caaattaata ttgttaacta tgaaaactct ataaatgaca
     2161 taatggatcc ttcaggttca ttctttgaat tagcaaatca aagttatgaa attaatgagt
     2221 tcatgttaaa aaacaaaaaa aattataaag aatggtcata tgaatatata gaattcttaa
     2281 ttgatcattt agatgaatta tgcaaatttg ttgattttga tataaaagat gtaatagata
     2341 ttattgatcc tacaataaaa acagatcttt cagaagaaca aaagaaatct ttgaatgata
     2401 aattaaagaa agtgtcttca agtgatactt taagtgaaga cattaagaag gagataaaaa
     2461 attgggaaaa caatttgaat agtttaaata tgaataaaaa taattctaat taactgatag
     2521 tgtaaaatgt acataattat aaataaaata gggatgtctt aaaattgatt attttctaat
     2581 ctaaatattt taggcctatc taaaaacata agattatggt ctaaaatttg aaattttaga
     2641 ccatgcttta tctttacaat aaatgggtct ttaaaataaa gtgtgtattc ttctaaaaag
     2701 tggtgataat taaaatatta aatatcacta tataaaggaa ggtgcacact ttttatgttt
     2761 aacaaaaacg aaatattaaa aaaacttatt gaagaatatg atgttaaaac aactacagat
     2821 attcaagata tgctaaaaga tttatttgcg agtacaattc atcaaatgtt agaagcggag
     2881 cttgacgacc atttaggtta tgatagatat ggcaataaga ataaaaatac taaaaactct
     2941 agaaatggat atagaaataa aaatgttaaa tcagattttg gagaaataac tttaaatatt
     3001 ccaagagacc gaaatggaga ttttgaacca aaggtaatta gtaattataa aaatgatata
     3061 tcaggaatag agaaccaagt gattggaatg tactcaaaag gcatgtctac tcgagatata
     3121 tcaagccacc ttaagagtat ttacggaatt gatatatctc attctctagt agctaaaatt
     3181 actgatagag ttctaccttt agctcatgaa tggcaaaaca gacctcttga tatggtttat
     3241 ccaattgtat ttatggatgc tatacattat aaagttagaa gtgaaggtag aataataaac
     3301 aaagctgcat atatagccat aggtgttaac ttagaaggta taa
//
LOCUS       DAMJVX010000264         3333 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.271, whole genome shotgun sequence.
ACCESSION   DAMJVX010000264 DAMJVX010000000
VERSION     DAMJVX010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.271"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            53..1765
                     /gene="ptsP"
                     /locus_tag="PC407_003515"
     CDS             53..1765
                     /gene="ptsP"
                     /locus_tag="PC407_003515"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDF2498920.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASIHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            complement(2557..3222)
                     /locus_tag="PC407_003516"
     CDS             complement(2557..3222)
                     /locus_tag="PC407_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498921.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
BASE COUNT         1276 a          358 c          543 g         1156 t
ORIGIN      
        1 tataaattta tagagtttat gtgctagatg atatttttta ggaggtaggg ttatggctta
       61 caaaggaata ggtgcttctc ctggtgttgc tttagggaaa gcgttagtag ttgaacatag
      121 tgaattagta attgaaaaaa aatctataga taatgttgaa gctgaaattg ctaaattaga
      181 agatgcagtt gcagtttcta aagaagaatt agtaaaagtt aaggaaaagg cttctgaaga
      241 attaggagca gaggaagcag aaatatttga agcacattta ttagttttag aagacccaga
      301 gttaattggt tctgcaatag ataaaattaa aactgaaagt gtaaatgcag agtatgcttt
      361 aaatgaaata aaagagatgt ttgttagcat gtttgagtct atggacaatg agtacatgaa
      421 agaaagagcg gcagatataa aagatgttac aaatagaata cttagacata tattaggaat
      481 taaagttgtt gatttatctg ctttaagcga agaagttgta ttaatagcac atgatttaac
      541 tccatcagac acagcaacta tgaataaaaa aatggtttta ggatttttaa ctgatatagg
      601 tggaagaact tctcatactg ctatcatgtc tagaacttta gaaatagctg caatcgttgg
      661 attaaatgat ataacttcaa aagttaagga tggagatttt gtagttttca atggagacac
      721 aggtgaagta attgtgaatc ctgatgaaga gactataaat aaatatactg aattaaaagc
      781 taagtatgaa gatgagagaa aagcattaca gctattaaaa ggaaaaccat ctgtaacttt
      841 agatggaaaa catgtggaat tagctggtaa cataggaaca cctaacgata tagaaggtct
      901 aataaagaat gatgctgaag gtgttggtct ttatagaaca gaatttttat acatggatag
      961 agattctttc ccaacagaag aaatacaata tgaagcatac aaagctgtat tagaaggaat
     1021 ggatggaaag ccgatagtta taagaacatt ggatataggt ggagataaag aattaagtta
     1081 tttaagtatg gaacctgaaa tgaatccatt cttaggttac agagcaataa gactttgcct
     1141 agatagaaaa gatatattta agactcaatt gagagcttta tatagagcta gtattcatgg
     1201 aagattaaga atcatgttcc caatgatttc atctttagaa gaattgttac aagctaaaga
     1261 agttgtaaaa gaagttttag cagaacttga ttctgaaggt gtggcttatg ctaaagatgt
     1321 agaaattggt atgatgatag aagttccatc tgctgctgta atatctgatg tattggctaa
     1381 acacgtagat ttcttctcta taggaactaa tgatttaatt caatacactt gtgcagttga
     1441 tagaatgaat caaaagataa gttatcttta caatcaattt aatccagctg ttttaagact
     1501 tataaagact gttatagaca atgcacataa agaaggaaaa tgggcaggta tgtgtggaga
     1561 atctgccgga gaccaaaaaa tgataccaat acttttaggt atgggattag acgagttctc
     1621 tatgtctcca atatcaatac ttcctgctag aaagttaata acttcagtta aagagtcaga
     1681 tatgaaaaaa ttagctgatg atgtacttaa tatgggtact gctgaagaaa taaaaagcta
     1741 tatagaaaag acttttaata tataattaat gttatatagt tactaaaatt catttttcta
     1801 aataacaaaa ttatatttta cgaattaaaa aggagatgtt tcaaaataaa aatttgaagc
     1861 atctcttttt tataaaagat tactaagaat tacttaatct ttgtaatagt cattttgtga
     1921 aaaagcaaaa ctgcattgtg actatagtat atatggatgt tattatcaat aaaagtacta
     1981 ttaaaatgaa gattcattct caaatgatat caaaagtagt tgtgatggag taagataata
     2041 ttttgtgatt taatagtaaa aattaaaaaa atatggtatt gaaaaataat taaaagtatt
     2101 taataataga attagggaag aataagttta taagtaaaaa taattgatta ctagttaata
     2161 aaataaatgt gatatccaaa taaatcctaa atctaatttg agttttaata tagatagtct
     2221 aaagtttgat tattaaagat ttaattgtaa gttgttacag ttgatgatat tgattagaga
     2281 ttaaagttta gagtagaaag ttaccttaaa tttaaaaatg caagataacc caatctaaaa
     2341 attataatca ctcgataggt ttaaggattt atttgtttat tataaatagg agggtgtctc
     2401 aaaatgaact ttttagttta tgaggcactc tcttttatat agaattaaat atattttatt
     2461 atttcaacaa tgaaaaagaa gcttatctct attttgagac agcctctttg tatactatta
     2521 ttaatttaat taataatata atttaaaatt ataatattag ttaagatttc ctgctttatt
     2581 agtaaataat tctttaatag cattgtttct ggaaactaaa tcgttatcag agttgtttgt
     2641 ttttttcata atcactgaat aacgtttttt atttataaaa aatattaaaa tcttatcatc
     2701 atcaaaattg tattctttat atctgtcaat agactcataa cctagattta gaatgttttt
     2761 gtcttttata ctatctacat ctaaaatata tgatgggtca gcctttagta atatattaat
     2821 taaacatttt tcttcaccag cacagaataa ctccatctta cttgatatag atttagattg
     2881 ctctgaagtt atttttgcag gaataaggtt gtcattatca tctcttagaa gataaccatg
     2941 tgtgtcttgt tgatatacta aattaacctt tttcttagga acactaatca ttttatctaa
     3001 atgtacataa tgatgatttt caacaaaacc ttcatttaat atttcaacaa agttattaat
     3061 tttacctagg caaaaagatt ctatagattc tttacagaat ttatttgtaa atggtattat
     3121 gtttacgttt tcctcatctg tattccatgc tatagcataa tccaacgaat atacttcttc
     3181 ctctatatct gcaaaattat attgtactat gtttcctttc aaaattactc gacctccatc
     3241 ttataatata tttaatatac tttataaaac ttatattata tttaaataat atcataaatt
     3301 ggataaatat gatatagtaa ataagaaaaa att
//
LOCUS       DAMJVX010000265         3277 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.272, whole genome shotgun sequence.
ACCESSION   DAMJVX010000265 DAMJVX010000000
VERSION     DAMJVX010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.272"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            184..831
                     /locus_tag="PC407_003517"
     CDS             184..831
                     /locus_tag="PC407_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2498922.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTAFKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(998..1567)
                     /locus_tag="PC407_003518"
     CDS             complement(998..1567)
                     /locus_tag="PC407_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498923.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(1572..2471)
                     /locus_tag="PC407_003519"
     CDS             complement(1572..2471)
                     /locus_tag="PC407_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2498924.1"
                     /translation="MKLLSKVNNNIRLIIQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(2482..2631)
                     /locus_tag="PC407_003520"
     CDS             complement(2482..2631)
                     /locus_tag="PC407_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498925.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(2624..3208)
                     /locus_tag="PC407_003521"
     CDS             complement(2624..3208)
                     /locus_tag="PC407_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDF2498926.1"
                     /translation="MKKFFMVVLGCIFVLGVISGCSKKESKPVEKNKSNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKNIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEEWKKIIDERLKMVKANE"
BASE COUNT         1145 a          563 c          368 g         1201 t
ORIGIN      
        1 tgttaaaaac taatagtaaa tcttatacta atcgcatatt tatgttataa ctatattgta
       61 actattttcg taatcttagg aaaagattac ttataaaatt aaccttatac aataatatat
      121 cgtataatct taattagcat aatagttaca atattaaaat ttaaatatgg gagtgatttt
      181 agtatgataa gacaaacacc attagagaaa cgatatgaca agctaggtcc tcatattgta
      241 acagcattca aaaaacgtta ttttgatgct tattactgta aaactagaaa tgaagctttg
      301 caacaaattt tagatttaat tcctcaaaat catgtagtat catggggagg atctgaaaca
      361 cttaaagaaa tgggcgtaca aactgctgtt agagagaaag gatttaaagt tattgataga
      421 gatttagcaa aaactccaga agaaagaact gaaattatgc gtcaagcact tctatgcgat
      481 acttttttga tgagtagcaa tgcaattagt gaagatggac aactctttaa tattgatgga
      541 aatggcaatc gtgtagctgc aatgatattt ggtccaaaaa atgttatagt tgtagctggt
      601 atgaataaaa ttgtaaaaaa cattgatgat gcaatacaaa gaactagaac aattgcatct
      661 ccagcagttg ttcagcgttt tcaagatgtc aatacaccat gctatattaa tggaagttgt
      721 atagattgca caagtcctga aagcatttgt gcatacatgg ttactacaag aatcagtcgt
      781 ccagcaaaaa aaataaaggt aatattggta ggagaaaatc ttggtctata aaatatacat
      841 acatgtaaat agctctataa ataataaaat catatgaaat aacttatttc taaaagtggt
      901 agaataaaag cttctacaag actttcattc taccactttt atattctttt aatttgtgtt
      961 tctatattat taattgctcc aattttcata tatattacta actattttta ttttcataat
     1021 ttttttgttc ttcatcaagc tgtttataat aatctttatt atagtcagct agataatcta
     1081 taccacctcc tgtaatcaca tttaataatt tcattgcaga ttcttgatta actttacaaa
     1141 cataattatc accagattta acaagctcta atgtaatatt aaattctttt ttctttgcag
     1201 aatcaatatc tttcatagtt ggatttggca tcatttctcc tagtttagga ggattctttt
     1261 ttaaaataga aaccatatca gtattgttaa tttttaaatc tacagtcact ttatccttat
     1321 tattctttat atctgaaatt tcccaagtca tatttttaga atacatttta aatccttctt
     1381 tatcttcgta tataagatta cctaaccctg attttgggaa atacttatca acagtatctg
     1441 catcttgtgt ttttattgaa ttaaacatac tatccacttc attttttatc ttattatcat
     1501 tagtacaacc agctaacacc agtactgata ttgcaaacat tataatacat agaataaatt
     1561 tcttcattaa attatatccc cctatacttt tttgcacaaa tattattctc ttttttatct
     1621 tttgaaaaag ttgtagtaat tgcattttgt ggacaaactt ttatacattc accacaccta
     1681 atacagtctg gactatttac atctttatat attggtatat caagcttaca ttttgtttga
     1741 catgctgtac aatttgtaca tttatcttta tcaattttaa atctatatat tgaaatagga
     1801 ttaaaaaaac catagattgc tcctaaaggg catatatact tgcaaaatgg cctataagta
     1861 atgattgaca tcaataatat caatagcaaa atacttaact tccagctaaa taaaaatcct
     1921 attgattctc ttaaattctc atttcctaaa ataagtggta ttcctcccaa aagtgttcct
     1981 gaagggcaaa tccatttaca aaaccaagga tttccacccc ctgctacatt tacaatagca
     2041 actggcaaaa taattacaaa cattattaag actatatact ttagatattt tagatattta
     2101 tcaccataaa ctttttttat ctttactgga aagggtactt tatgtagtaa atcttgtaca
     2161 agtccaaagg gacaaagcca accacaaaca aatctgccaa aaaaagcacc aaatgccatt
     2221 aaaaaaccaa caacataaaa tgagaattta tactctctgc ttgataaaac tgcttgtaat
     2281 gaacctattg gacatgctcc aagtgcacct ggacatgaat aacaattcaa tccaggtaca
     2341 cataattttt tactttctcc tccatatata gttccttcta aaaatccatt tacatatcca
     2401 tttgttaatg ctgtaaatgc aacttgaata ataagtctta tattattatt taccttagat
     2461 aataacttca ttcctcttct cctaaccaat accaatacac tcaagacaaa tcataattgc
     2521 tttagttaat actgtatttg tttctccacg atatgtacct aatgcaataa atataatact
     2581 aattgcaata atagaaatgc cttttttatt atctttaata aagttactca ttggctttta
     2641 ccatttttag tctttcatct ataatttttt tccactcttc cttactattt gcacctacaa
     2701 ccttctcacc cacaaagttt cctttcttgt ctacaaaata agttgttgga aactccataa
     2761 ctcttttaaa atcagttttt ttaagttctt cagatacagt caattgttgg taagttgcat
     2821 ctgacttttt cataatgttt ttaactaaat ttttctcttc atttgaaaga ccagttctca
     2881 tatcagtttt atcaacttct acaactaatc cctttatagc tacattggaa ttattactct
     2941 catattcttt tgataatgct tcaagctctg gcatttcacc cacacatggt ccacaccaag
     3001 tagtccagac attaataaga gtaagttctt tatttgaaaa tatctctttt gttaccttat
     3061 ttccatcaac atcctccgta tctaaattac taaaccattc actattactc ttgttttttt
     3121 ctactggctt agattctttt ttgctacatc cagaaatcac acctaaaaca aaaatacatc
     3181 ctaaaactac cataaaaaac tttttcatat ctcacactcc tagaatttat atagtatata
     3241 aaatggctgc ttgaaataaa aataacttct cttctat
//
LOCUS       DAMJVX010000266         3213 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.273, whole genome shotgun sequence.
ACCESSION   DAMJVX010000266 DAMJVX010000000
VERSION     DAMJVX010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.273"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     misc_binding    138..400
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            476..3142
                     /locus_tag="PC407_003522"
     CDS             476..3142
                     /locus_tag="PC407_003522"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDF2498927.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKAHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKVKKEKFEEMIKSVEERLTNLESKIK"
BASE COUNT         1303 a          356 c          619 g          935 t
ORIGIN      
        1 acagaataac tgtaaaaaca tattttaaaa tataacaact atataaaaga gaatagacat
       61 aattaagaaa attcagaaaa aataaaataa aaataattga caaagagaat aatatttgta
      121 tataataata tgtaatatta aagactgaga agaagaggag taagtaaata ctactaaaag
      181 agaggaaaat tcactggctg taagattttc taagtataag atttactgaa tgtagctttg
      241 gagtttctaa actgaactat aagtaagttt agacggttag aaccgttaaa ttctttgaga
      301 gtcaaagttg ataaaataac attaactaat aatttaatta tatgttaatg tattttaagc
      361 tttggaatta aggtggtaac gcgagctttt cgtccttttt aaagaggatg aagagctctt
      421 ttttatttct aaagatattt aaaaaattta tttataaaat acaggaggca ggacaatgga
      481 aaatacgaat ttatcaaaaa cttataaccc aaaagatttt gaggctaggc tatataaaaa
      541 atggatggat gaagggtatt ttaaatctaa accaaatcca gataaaaaac catttacaat
      601 aatgatgcca ccaccaaata taacagggca attgcatatg ggacacgctc ttgaccatac
      661 acttcaagat atattaatca gatggaaacg tatggatgga tatgaagctt tttggcttcc
      721 aggaactgac catgcttcta tagcaacaga agttaaagtt gttgaaagaa taaaaaaaca
      781 agaaggcaag acaaagtatg aaataggtag agaagaattt ttaaagagag cctgggaatg
      841 gaaagatgaa tttggaggaa aaatatctaa tcagcttaaa caactaggtg attcttgtga
      901 ttgggataaa gaaagattta ctatggatga agggtgtaat gaagcagtta tagaattttt
      961 tgtaagccta tatgaaaaag gtcacatata cagaggaaat agaataataa actggtgccc
     1021 agattgcaaa actactttat cagatgcaga agttgaacat gaagaacacg acggaaactt
     1081 ttatcatata aagtacccac taaaagatag tgaagacttt ttagaaatag ctacaactag
     1141 acctgagaca atgataggag atactggtat agcagtaaat ccagaagatg atagatataa
     1201 acatttaata ggaaaaacag caatacttcc attagtagga agagaacttc caatagttgc
     1261 agatagttat gttgatttag agtttggaac tggtgcagtt aagatgactc cagcacatga
     1321 ccctaatgat tttgaagtag gattaagaca taatcttgag cagttaaata ctatgaatga
     1381 agatggaact atgaatgaag tttgtggaaa atatgaaggt atggatagat ttgagtgtag
     1441 aaaagctatc gtagcagact taaaagaaca aggttaccta attaagataa aagcgcataa
     1501 tcataatgta ggaacttgtt atagatgcca tacagtagtt gagcctagat tatcagagca
     1561 atggtttgtt aagatggaag aacttgcaaa accagctata gatattctta aaaaaggaga
     1621 attagaattt gttccagata aatttgataa aacttattta caatggctag aaaatataag
     1681 agattggtgt atatcaagac aattatggtg gggtcatcaa ataccagctt attattgtca
     1741 agagtgtgga gaaatagtag ttgctagaaa aatgccagat aagtgtccta aatgtggaag
     1801 cactcatttt aaacaagatg aagatgcctt agatacttgg ttcagttctg cattatggcc
     1861 tttctctact ctaggatggc caaataagac tgaagcttta gattattatt atccaacaag
     1921 tgtacttgtt acaggatacg atataatatt cttctgggta gttagaatgg catttgcagg
     1981 tatgttctgt atgaatgaaa agccatttga ccatgttttg gttcatggat tagtaagaga
     2041 ttctcaaggt agaaaaatga gtaaatcttt aggaaatggt atagacccat tggagataat
     2101 cgagcaatat ggagctgatg ccctaagatt tacattaaca actggaaatt ccccaggaaa
     2161 tgatatgaga ttctatatgg aaagagtaga atttgctaga aactttgcaa ataagttgtg
     2221 gaatgcatca agatttgtat ttatgaatat tgatgaagat ataattaaaa acatgactag
     2281 agaaagtgtt aaagaggact taactctagc tgataaatgg ataatatcaa gagcaaataa
     2341 tattgtaaaa gaagcaacta ataacatgga taagtttgat ttaggaatag cacttcaaaa
     2401 gatatacgat tttacttggt cagaatactg tgactggtat attgaaatgg ttaaacctag
     2461 attatatggg gaagatgcaa atgctaaatc tgcagcatta tatacattaa cttatgttct
     2521 tgaaaaaata ttaaaattat tacatccata catgccattt ataacagagg aaatatatac
     2581 tcatcttcct actgttgaag gatgtataat agtatctgaa tggccaaaat ataatgaaga
     2641 agataacatg gctgaagaag aagatatgat gaacttattg atggaaggta taagaagtat
     2701 aagaaatgtg agagcagaaa tgaatgtacc accttctaag aaagctaaat tgataataat
     2761 cccaagtgaa gaaaaaatag aagctattga gcttggtaaa gactatttta taactctagc
     2821 atctgcttca aatgtagaga tagcaaaaga taaatctaat gttccagaag atgcagttgg
     2881 agtagttatt gatggtgttg aaatatttat acctcttaat gaattagttg attttgaaaa
     2941 agaaattgag agattgtcta aagaaaagaa aaagttagaa ggcgaaataa agagagttaa
     3001 tggaaagctt gcaaatcaag gtttcttagc aaaagctcct gaaagtttaa ttgaagaaga
     3061 aaaagtaaaa aaagaaaaat ttgaagagat gattaaatct gttgaggaaa gattaacaaa
     3121 tttagaatct aaaataaaat aaatataaga attagtaaaa acatacaaaa agtatatatt
     3181 ctatactttt tgtatgtttt tctttatata tat
//
LOCUS       DAMJVX010000267         3208 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.274, whole genome shotgun sequence.
ACCESSION   DAMJVX010000267 DAMJVX010000000
VERSION     DAMJVX010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.274"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     misc_binding    91..301
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            527..1342
                     /locus_tag="PC407_003523"
     CDS             527..1342
                     /locus_tag="PC407_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2498928.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            1370..2047
                     /locus_tag="PC407_003524"
     CDS             1370..2047
                     /locus_tag="PC407_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2498929.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            2106..2828
                     /locus_tag="PC407_003525"
     CDS             2106..2828
                     /locus_tag="PC407_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2498930.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         1279 a          328 c          599 g         1002 t
ORIGIN      
        1 ataatttaca agtaaaaatt attaaaaaaa taaaaaactt attgacaaaa atattataag
       61 gaagtacaat tgtattaaca atttaaggaa acaaatccaa tgatagagaa agtagcatat
      121 atggatttac agcgagttag gggttggtgt aagcctagca atgaagtcta tgtgaagaga
      181 gctctggagg agattatcag aaactttagt atgataattg gtttacccgc cttaagggat
      241 tgagaggata agtacttact tatcaaaaag agtggtaacg cggatataat tcgtctctta
      301 gctgtaaagc taagggactt ttttgattta aaaataaaaa tatccataca gcttaaatgc
      361 taacagtcac aaattttgaa agtaatataa gtatttatca ataagagtgg taacgcggta
      421 aagtcgtctc ttagtcatta aagactgaga gacttttttt gtaactcgaa acatttaagt
      481 attaaaatct gagggaaatt ataattaaaa gagaggggta gttaaaatga aagtatttaa
      541 aaaattatta agtttaggat tagtattagg attgacatta tcgctagtag gatgtagtgg
      601 tggaggagaa aaaacaaagt tagaacaaat aaaagacaat ggaaaattag ttgttggtac
      661 tagtgcagaa tttcctccat ttgaatttca taaggtagta gatggaaaag attcaataaa
      721 aggattcgat ataatgttgg ctgaagaatt tgcaaaagag cttggtgtaa aggttgaaat
      781 taaggatatg tcatttgatg gattaatagg tgctttaaat gcagaccaag ttgatattgt
      841 tcttgcaggt atgtctccaa caccagagag agaaaagagt gttgattttt cagaattata
      901 ttatttaagt agaaatgcag ttatagttaa agatgcagat atagacaaag taaaaactga
      961 agatgattta aagaaactta gagttggagt tcaagcagga agtatccaag aagaatatgt
     1021 agttaacact ttaaaaatga caactacaaa atctttaaaa gcaatacctg atttaattac
     1081 agaattaaaa aatggaaaca tagatgcagt tgttactaat gaagcagttt ctttaataaa
     1141 tgttaaaaaa tatgatggaa taaaaatggc aaatactgaa gttggtaaag atgtaacaga
     1201 aggtatggca gcagcaatca aaaaatctga caataacaaa gattttatag aattattaaa
     1261 caaaaagata aaagaattac aagatggtaa gaagatagaa gaatttttaa atgaagcatc
     1321 tactgaagct gcttcaaatt agtaagaaag ttatgtggga gatgaaatta tgagtttaga
     1381 ttttagtttt ttaagtagat ttgggacttc ttttttggaa ggaacaggtg tgacagtatc
     1441 aatttctctt gtggcattat gctttggatt tataataggt ataattatct gtatggcaaa
     1501 aatatcaaag agcaaagtat taagagcaat atcatcaatc tatattgagg ttttaagagg
     1561 gacaccatta ttagtacaga tatatatagt atggtttggt ttacctcaat taggaataag
     1621 attccctatg ttgtttggta taccaagtga atttatagct agtgcatttg cattatcagt
     1681 aaactctggt gcatacgtgg cagagatact tagatcaggt atacaatcag ttgataatgg
     1741 acaaatggaa gcaagtagat cattaggatt aaattattgg tcaactatga gatatataat
     1801 aatacctcaa gctataaaaa atatattacc ttcattagca aatgagttta ttacacttgt
     1861 aaaagaatct tcaataatat ctgtaatagg agttgttgag ataatgcgta ctgctgatat
     1921 agtaaaaaat gcagcattta gagcattaga gccattaata gtagcagcag caatttactt
     1981 tgttataaca tttactctat caagattagt tggattatta gaaaagaaac tatctgtgtc
     2041 taactagatg agagcatgtt tagcatttag ggaaaacata gaatagtaaa aaggaaagtg
     2101 ataatatgat tacaataaaa aatttaagta aatcttttgg agatttaaat gtattaaaaa
     2161 atatagactt agaaatcgct aaaggcgaaa taatggttat agtaggtcca agtggttctg
     2221 gtaaaagtac acttcttaga tgtatgaatt tactagaaat acctacaggt ggagaaataa
     2281 tatttgaagg aaaaaatttg gtcgataaaa aaactaatat tgatgaggtt agacaaaata
     2341 taggtatggt gtttcaaaac tttaatttat tcccacataa gactatttta gataatataa
     2401 cattagcacc aattaaatta aaaaagatga ctaaggaaga agcagaaaaa aaagcagaaa
     2461 ttttgttgag tagagttggt cttttagata aaaaagattc atatccatca caattatctg
     2521 gaggacaaaa gcaaagaata gcaatagcta gagcacttgc tatggaacca gatatgatgt
     2581 tgtttgatga gccaacatct gctcttgacc cagaaatggt aaacgaagtt ttggatgtta
     2641 taaaagaact tgctaaagaa ggtatgacaa tggcaattgt aactcatgag atgggatttg
     2701 ctaaggaagt tgcagataga gttattttta tagatggagg aagtatactt gaagataata
     2761 ctccagaaga agttttcgga aatccaaaac atgaaagaac aaaagcattt ttagcaaaag
     2821 tgctataata tataaaaagg atgttattga tattagaaat tcaatacatc cttttttagt
     2881 gatggttaat attaatttta aaattaaagt tatattaata ttaactttat ttttacgatt
     2941 ttttgcagaa aaaaaaagag ctcggggtaa gctctttttt tccaccgaat taatttccgt
     3001 tactaatccg atttttaata aatataaaat atatccttat gaaagggtat tgggaataga
     3061 tatattaact aactagggtt accaggggga taaccttaat ttattataac aaaatgcgac
     3121 acaaaaatca atattttttt tgaaataaat tataaaagtt atttttataa caagtaagct
     3181 tataaattta acttttatag taaaaata
//
LOCUS       DAMJVX010000268         3200 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.275, whole genome shotgun sequence.
ACCESSION   DAMJVX010000268 DAMJVX010000000
VERSION     DAMJVX010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.275"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(271..528)
                     /locus_tag="PC407_003526"
     CDS             complement(271..528)
                     /locus_tag="PC407_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2498931.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            1108..1329
                     /locus_tag="PC407_003527"
     CDS             1108..1329
                     /locus_tag="PC407_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HDF2498932.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            2021..2251
                     /locus_tag="PC407_003528"
     CDS             2021..2251
                     /locus_tag="PC407_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDF2498933.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            2264..2413
                     /locus_tag="PC407_003529"
     CDS             2264..2413
                     /locus_tag="PC407_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HDF2498934.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            2670..3107
                     /locus_tag="PC407_003530"
     CDS             2670..3107
                     /locus_tag="PC407_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HDF2498935.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
BASE COUNT         1289 a          404 c          339 g         1168 t
ORIGIN      
        1 ttgctatttc tttatttata tagtttattt atttattttt cccctttata caagcaattg
       61 tatcatttta tctatttttt agccaacaaa tattttcgac cattttcttt gtattctcaa
      121 tatatctcca gtatttaaca aattttttta tagaatatac aatgtattta aattaataag
      181 tagtaaattt agttaatttg tctaaaaaaa ctttataaaa catcttcttt gtctttactc
      241 ttacatttaa caaggctatt ttcattatgt ttaaacatta ttttcattga gtattcgctt
      301 aatattgctt acacacatat tatatctaat agccaattca ttatggttga ctccattaaa
      361 ttgttttact atatccctat ttctagcata tctaaaaagc cccttatacg taggtatata
      421 agtatttgtt cctccataca acttggcaac ctctatgaac ttatcaattc cgattatttc
      481 taccattgca tgaaattgaa ctggtatatc ctccactttt atcttcataa gttacacctc
      541 ctttaagata tttttataga cttaacaaag tttttattta atacattatt gactaaacta
      601 tatttattag ttttattgaa tctaataaaa aatttcttag ctttaaaatt aatttttgta
      661 ttaactttaa taaaatttct cataacttat aactttgtta gctttaataa agattttaat
      721 ggtttaaaag taaaatgtta atattggact tttaactttt ttatttacag attaaattta
      781 ataaaatttt taacataagc tttaacattt aatttaacat aaactttggt acctaacatt
      841 taactctaca catatgaaaa tccatacaaa actatctcaa ctaagcatga atatatacaa
      901 atactatttc aaaacactaa gaacatatct tcaaaaataa cttgtaaact ttttcatcat
      961 cagaaaagta aaatttttcc tataaaaatt tacacaaaaa atctttagaa attactgttt
     1021 aactccgata ttatcttaga tgatagtatt atagctaaca aagaaagtcg acaaatcttt
     1081 gaaattaaat ttttaggagg taattttatg gaagttgttt caacaaaaaa tttatctagt
     1141 ataaaactaa aattggatgc tggttttgat gataagggta aggcagttgt taaaagtaaa
     1201 tcatttgcaa atgtaaaagc agaggcatta aatgaagatg tttatgctgt tgcagaagca
     1261 atagcaagtc ttcaagagaa ccctattgta gatattttaa aattagatag tacatcatta
     1321 tctaaatagt atcttttaaa cgtactctgc tttacaaatt aaaaaaatta actctttata
     1381 taaaagatta agttatataa cgcacttaat acgctataga tttggcagat tataacttca
     1441 attttggtta taactttaat atattaatgc tttgaaaaat taatatatta aacgtatttc
     1501 aacttaaaca tgttagctat tttaaatgct accctacttt aatctaatta attgaaataa
     1561 attaaatatt ttctatatca gcaataacta gagtcaacaa ttaaaatact aatattgaca
     1621 attgaaacac tgatactaac aaacaaaaca ctaatattga caattgaaac actgatacta
     1681 acaaacaaaa cactaatatt gacaattgaa atactaatac taacaagcaa aacactaata
     1741 ttgacaattg aaatactaat actaacaagc aaaacactaa tattgacaat tgaaatatta
     1801 atactaacaa gcaaaacact aatattgaca attgaaatat taatactaac aagcaaaaca
     1861 ctaatattga caattgaaat actaatacta acaagtaaaa cattaatatt gacaattgaa
     1921 atattaatac taacaagcaa aacactaata ttgacaatta aaataccaat attaacaagc
     1981 aaacaattaa aatataaatt attatatcgg aggtaattat atggaaatta agaaaaaatt
     2041 agtcatgagc tttaaaaatg taaaggataa acaagtttct ttttctatag aaaatccaaa
     2101 ggatgattta actgaagaaa atataaaatc tgtcatggat ttgatagtat ctaaaaatat
     2161 tttttcggtt ggaggatttg acctagcttc tgttgttgaa gccaaggttg tggaaacaaa
     2221 taccactcct tatgacttag ttatagaata gggggctaat tttatggatg atagtttaca
     2281 actgttaata accaatgttg gttttcctat agctctatcc atgtacttgt tggttagaat
     2341 tgagggtaaa ttaaatactt tatcaaacag tattgataca ttatccaaaa atattttgat
     2401 atttaagaag taatttgaca atatatgtaa gtcataaaat aggtctattt gtaaaataat
     2461 tagacatatt ctatgctaaa tactaggata tttggataat ttatccaaat atccatagta
     2521 tttttttgaa ttttaccacc tcaaatttga atttcactat tttatttttg aatttcacta
     2581 tgcttaattt gaaatttaga acgcatctag ttggactcaa taagatataa tgctattcta
     2641 atgacagtaa taaaatgagg tgatgattaa tgaaaataca tgtcgaacaa ggtattaatt
     2701 gtgatgatat tgaaataacc ataaaatgtt ctgctctaga taataacatt gaaaaaataa
     2761 tttcattatt gagtacacca ttacttgata taaaaggtaa aaaatcagga gagatttata
     2821 aactcaatgt ctgtgatatt ttatactttg aaacagttgg aaataaaaca tttgcttatt
     2881 atgaagatga tgtatttgaa attgatttaa aaatatataa attatcaaaa ttattagcta
     2941 atacatcctt ctcacgaata tctaaatcca tgattttaaa tatggactac ataaaaagta
     3001 taaaagtaat atcacataat cgcatgcaag ctactttaaa aaacaacgaa aaagttatta
     3061 taaatcgtcg ttatataaaa aatattaaag aaaagctaaa catttaataa aaatctaaag
     3121 taatttatta atatcactaa ttgcaaattt attgacctat ttaataatta ataataatat
     3181 aatatgttca atattaaata
//
LOCUS       DAMJVX010000269         3199 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.276, whole genome shotgun sequence.
ACCESSION   DAMJVX010000269 DAMJVX010000000
VERSION     DAMJVX010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.276"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            1..612
                     /locus_tag="PC407_003531"
     CDS             1..612
                     /locus_tag="PC407_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDF2498936.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIIEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            910..1248
                     /locus_tag="PC407_003532"
     CDS             910..1248
                     /locus_tag="PC407_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HDF2498937.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(1655..3091)
                     /gene="miaB"
                     /locus_tag="PC407_003533"
     CDS             complement(1655..3091)
                     /gene="miaB"
                     /locus_tag="PC407_003533"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDF2498938.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT         1100 a          475 c          364 g         1260 t
ORIGIN      
        1 atgataaacg atattaaaaa taaattcaaa aattacaaac catatataaa tggatacaag
       61 aatatgaaga gagcatctgt tttaattcca ataatagaaa taaataacac acactatata
      121 ttatttgaag ttcgctcaaa aaatttgaag catcaaccta gcgaaatttc ttttccaggt
      181 ggtaaaatag aatcaggtga gactccatat gaagctgtta ttagagaaac ttgtgaggaa
      241 cttggaactt ttagtgacaa tatagaagta atatcaagtc ttgacctcct tataacacca
      301 gtaaagttta tcatacatcc atacttagga tatataaaaa atattaataa tttaaatata
      361 aacaaggatg aagttgacca tacttttttg gttcctataa aatatctttt agagaatcct
      421 ccaaatactt atagtaatac tgtaaaagtt tttccagatg aaaatctacc atatggttca
      481 attccaaacc aaaaagatta taaatttgaa agtggaagtt atcctattct gttttataaa
      541 tataaagatt atgtaatttg gggaatgaca gcaaaaatac tagaaaactt tttaaatttt
      601 ttatcagaat aacactcata ataaatcatc aaaactttta cataagaaat ttataaaagt
      661 ttctaaattt aacaattcta aaatatcaat aactatattt tgtagcactt ttttatttta
      721 ttattgtttg aatatttgat ggttttcaat gcaatttcaa cataaatact atgtattagt
      781 gtatactgtt gatgaattaa agtatgataa tctatttaat aaagtatgaa gtaaatgatt
      841 ttaatgtgtc tttatggtaa aattactaat aagtaataaa attaattaaa atatatggag
      901 gtgcttataa tgtttgctgt aggatttgtt gttataattg cacttgcctt tttcatttta
      961 agtgtactat tatttaatgg aaaagccagt atgctactag caggttataa ctctatgagt
     1021 aagaaagaaa aagcacgata cgataaaaaa aaattatgta gaagttctag cattgttact
     1081 tttattgtct ctataatgct atttattatg gcatttttgg gatatagaat agagagtggt
     1141 caaatggacg aaaaaaaaat ggttcccttt tctataatat ttataatagt catactttcc
     1201 agtgtatttt ttaatatcct ctactctaac aaaaaatgta aaaaataaat agttgtaagt
     1261 tgagcagtta attataaaag attgactgct ttttcatgta tattgactac tcttattaaa
     1321 tattttaaca tttaaaaatc tatgtaaaaa taatgaatat agtacaaact tctaataatg
     1381 attttacctt tgtggttaca gtccactata tataatttaa tatgataaca tggaattact
     1441 aatgacaact aagtaagctt ttataaaata taataaaaca aaaatagatg tacaatgaaa
     1501 ttatatctta tatcaaattt atttttatta atagcaaaaa cttacattag cataacatat
     1561 gacaaattcg cttaaaataa aaatttttaa tataatttgt tctatagtac atctatttcg
     1621 attagtttac ataattttat tattttgtac aatattaatt atttactaat atcccattta
     1681 atgaaaaagt tctaggttca gtaattttga ccataactaa tttaccaatt aaatcatcat
     1741 ttccaccttc gaaatttacg agtttatttt gtctagttct accagcaaac ttattttcat
     1801 tatttttact tcttccttca actagaactt caactattct atctttatat ccatcattta
     1861 tttctgcaga aatcctattt acagcttcta acaccctatt aaatctatca tgctttatat
     1921 cttctggtat ttgattttcc attttagcag caggagttcc ctgtctttta gaataaataa
     1981 atgtaaaagc tgaatcatat ctaacttttt caaccacatc taaagtgtcc aataaatctt
     2041 cttctgtttc tcctgggaaa ccaatcatta agtctgtaga aaaagcaata cctggaactt
     2101 cctttttagc tttttctatt atttctaaat aatattcctt tgtataatgt ctattcattt
     2161 ttttcaaaag gctactgctt ccacattgta ttggtagatg taaaaactca cacaccttat
     2221 cacaatctct aattgcgtat attacttcat cagatatatc ttttggatga gatgtcataa
     2281 atcgaaccct ttcaattcct tctatatcat tcacttttct taaaagttct gagaatgaaa
     2341 ctggattttc aagtgtctta ccataagaat ctacattttg tccaagtaaa gtgatttctt
     2401 tcgttccatt tgcaacaagc tcttttatct cgttaattat atcttcagga gttctacttc
     2461 tttctcttcc tcttgtgtat gggactatac aatatgtgca gaaattattg catccataca
     2521 ttatatttac aaatgctttt aattcaaatt ttctattact tctaagacct tctattactt
     2581 ctccatccac atcccataca tccactaaca ttttgtcaga atttattgat tctgtaagta
     2641 attgcggaaa tttatataga ttatgtgttc caaatataag gtctacatgt ttatattttt
     2701 ttcttaattc ctctacaaca tgtggttgtt gcatcataca tccacataca gctattttca
     2761 tatctggatt ttttcctttt agatgtttta attgtcctaa gttaccatac acttttagct
     2821 ctgcattttc tctaactgca caggtgttat atattattaa atcactttct tcaaccatca
     2881 ttgacatttg gtatcccatc tcttctaaca tcgaacataa tttttctgaa tcatgttcat
     2941 tcatttgaca cccaaaggtt tgagtaaaca cgagttttct ctttccagtt ctgttgaagt
     3001 attcttcatt ttgtctgtgt atttgttcta tatatttatc ttgttcttgt attttctcta
     3061 taggtattgt tacttgtttt cttttgctca cgtctattcc tcctagttaa tttcttatat
     3121 tacacataac cttaattata tcaaataaga aaaatctttt aaagatatta ttatttttct
     3181 agttgtgttt taatagtaa
//
LOCUS       DAMJVX010000270         3196 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.277, whole genome shotgun sequence.
ACCESSION   DAMJVX010000270 DAMJVX010000000
VERSION     DAMJVX010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.277"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(145..666)
                     /locus_tag="PC407_003534"
     CDS             complement(145..666)
                     /locus_tag="PC407_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2498939.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENIIIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
     gene            794..2158
                     /locus_tag="PC407_003535"
     CDS             794..2158
                     /locus_tag="PC407_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2498940.1"
                     /translation="MKESNKKIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDKLHIAIKTSILWTTIFCVVLGL
                     ILVLFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            2347..3024
                     /locus_tag="PC407_003536"
     CDS             2347..3024
                     /locus_tag="PC407_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2498941.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSDYKKGNPVH
                     CGDSDCIGILIIKSGTIRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQINSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
BASE COUNT         1005 a          446 c          503 g         1242 t
ORIGIN      
        1 aaaagtttaa cattctccct caattaagtg agctttattt taaatatact tttcagttta
       61 tatcatttgt ttcatttttt ctaatatctc attattaaat atgctggttt ttgtttaatg
      121 attattaagt tagcttctct aattctaatt ccccatatta tcttttaaag aatttatcca
      181 ggtttccaat gcttgtgcta gcaccaactg ttgctccaac actgcttttg ctgtctctgg
      241 tacttctgtc tttataatta tattttcctc aatagttttc tttaactctt gaacattctc
      301 ttcaatatta ttgagacata atttctgttt ttctggtgta aggctattta aatttactat
      361 aacagcatta aaatctaaaa gaatatttat tggtttgcta gatgtatcaa acattaaatc
      421 taaaaaatgt gtctcaccta attcagttaa tgaataaact gctttttctg gcatttttcc
      481 attcttcaca gttttacttt caatatatcc cttttgttct aattgaatta cttttttata
      541 aattgatggt gtacttattt taacccaacg tgatatattc ctgtattcca ctgccttttg
      601 aatatcataa gcactcttgg gttgttgctt tactattccc aatacaatta aatctattgt
      661 agccataaaa atcatctcct ttagttttct tactattgac aaatactata aattatagta
      721 ctattttcca tacttactat aatttatagt attataattt agacttaatg tcaatagaag
      781 aggagtgtta agtatgaaag aaagtaacaa aaaaattgaa ttattagggt ctgccccaat
      841 ctcaaaagca ttgttatcta tgggattacc taccatgata ggtatgatga taactgcatt
      901 atacaatttg gttgatgcat actttgtggg tggacttgga acaagtcaaa tgggagctat
      961 atccgttgca tttccattag gtcagcttgt tgtgggatta ggattattgt ttggaaatgg
     1021 agctgcttca tatattgcca gacttttagg ccatggtgat aatgaaaaag caggaaaagt
     1081 ggtaagtaca tctatttaca gtagtattat agtgggtaca ttagttatta ttgtgattat
     1141 gatttttctc aatccaattt taaaattgtt aggagctaca aaaagtatta tgccttatgc
     1201 aatatcttat gctagcattt atgtcgtgtc atctattttt aatatattca atgtcactat
     1261 gactaatatt gtcacaagtg aaggagctgc taaaacaact atgtgtgcta tgttattagg
     1321 tgctttgctt aatatgattt tagacccgat attcatttat acttttggtc tgggggtaag
     1381 gggtgcttct attgcaacag ctatctcaca gattgcttca acacttgttt atgtaggata
     1441 tattttaagc aagaaaagta tatttagttt tagaattaac gaatgctgtt tttcaaaaga
     1501 aattttttca gaaatcctaa aaattggcat acctacatta atatttcaat tattgactag
     1561 tcttgccatt acattaacta atatggaggc tacaaagtat ggtgattctg tgatagctgg
     1621 aatgggtgct gtaacacgaa taacatcttt aggtagtctt gttgtatttg gttttataaa
     1681 aggtttccaa ccaattgctg gttataattt tggttcgaag aactacgata aattacatat
     1741 agcaataaaa acttccatat tatggactac aatattttgt gtagtgcttg gtcttatatt
     1801 ggtgcttttc ccaaaatcta taatttcaca atttacaagt ggtgacattc tattaataga
     1861 aaatggtagt aaagctctta gggcaaatgg gttttcattt atgctatttg gattctatac
     1921 agtgtattct acattattcc tagcactagg aaaagcaaag gaaggtggat ttttaggtat
     1981 gtgtagacaa ggcgtctgtt ttatccctat aattttgatt attcctgtat tctatgggtt
     2041 aaatggtatc ttatatgcac aaccaatagc tgatttgatg gctgctataa ttgctggttt
     2101 tatgtctata aggttacata aggaacttaa tcttttaaaa ataaacggta tgcattagtt
     2161 atacaaacct actatcatca aaacatatta ctttctcatt aatagcaaga atatatatta
     2221 tcatattttt tctacataaa tcatactatt actatctgac taaataatcc tcttaacaaa
     2281 ctacttgtca atttatctat tcacttgtat catgggatta taacaattta agaaaggaga
     2341 tattgtatga tttcacaaaa tgaaattgat atacttacac atagcttgcc tttttgggat
     2401 aaacttactg acttacaaaa agaattatta atttcttcag caaatacctc ggattacaaa
     2461 aaagggaatc cagtacattg tggtgatagt gactgtattg gtattttgat tataaagtct
     2521 ggaacaatac gtacatacat actttcagat gaaggtcgag aggttacact gttccgactt
     2581 gatgatggag atgtatgtat tttatcagct tcctgtattc taaaaacaat aacttttgat
     2641 gtctatgtag atgctgaaac ggactgcgat attatacaga ttaattcctc tgtttttgca
     2701 aaattatcta cagaaaatat tcatgcagag ttattttcat ataaattagc taccgaacgt
     2761 ttttctgatg ttatgtgggc tatgcaacaa attcttttta tgagttttga taagcgtttg
     2821 gcatcttttt taattgatga gatagctaaa aatggcagta gcactataaa tatgactcat
     2881 gagcaaattg ctaagtatat gggtagtgcc agagaagttg tatcgagaat gttgaaatat
     2941 tttgctagag aaggtattgt gtctttatct cgtggtggta taaaagtatt ggataaggat
     3001 aaacttcgtt ctttgacact gtaaaagtgt gtctcaaaat gaacttttta gttcatgagg
     3061 cactcttttt tgtgtgaaat caaatatatt ttcataattt caataataaa aaagatactt
     3121 atatctattt ttatatagta tcttatttga tatttacatt tattttttat gtatattttt
     3181 atattttaat aattta
//
LOCUS       DAMJVX010000271         3187 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.278, whole genome shotgun sequence.
ACCESSION   DAMJVX010000271 DAMJVX010000000
VERSION     DAMJVX010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.278"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            182..1228
                     /gene="recA"
                     /locus_tag="PC407_003537"
     CDS             182..1228
                     /gene="recA"
                     /locus_tag="PC407_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDF2498942.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            1404..2945
                     /gene="rny"
                     /locus_tag="PC407_003538"
     CDS             1404..2945
                     /gene="rny"
                     /locus_tag="PC407_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDF2498943.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT         1336 a          332 c          645 g          874 t
ORIGIN      
        1 cattttaaga atattttctt ttgtgtatat aattattaat aatggtttat aaataaaatg
       61 taatgaataa aaatataatc attagtagcg tttttaaatt gactaggttt gatttatgtt
      121 atataattaa tatataacaa gagaacaaat gtttgtagat aaatgaaggg atggtataga
      181 gatgagtgta gatcaagaaa aattaaaagc gttgaatgaa gctttaggta aaattgaaaa
      241 agattttggt aaaggttcag taatgaaatt gggagaagca acatctatgt ctatagatgt
      301 tatatcaaca ggagcgattg gtttagacat agctattggt ataggaggtc tacctagagg
      361 gagaatagtt gaagtatatg gtccagaatc ttctggtaag actactgttg cgcttagttg
      421 tgtagcatca gctcaaaaag atggaggaat agctgcattt atagatgcag aacatgcact
      481 tgacccagta tatgcaaaag ctttgggtgt ggatgttgat aacctaataa tatctcaacc
      541 agatacaggt gaacaggctt tagagatagc agaggcattg ataagaagtg gagcgataga
      601 tataatagta atagactcag tagcagcatt agttccaaag gctgaaatag atggagatat
      661 gggtgattct catgtaggtt tacaagctag acttatgtca caagcactta gaaagttaac
      721 tggttcaatt aaaaaatcaa attgtgttgc tatatttata aaccagttaa gagagaaagt
      781 aggaataatg tttggaaacc cagaaactac tactggagga cgtgcactaa aattctattc
      841 atcagttaga ttggatgtta gaaaaataga tacaataaaa caaggtgata aagttatagg
      901 tagtagaact agagttaaag ttgttaaaaa caaagtagca ccaccattta agcaagctga
      961 atttgatata atgtatggag aagggatttc aaaaattgga gaccttttag atatagctgc
     1021 tgatgtagat atagttaaaa aatcaggttc atggtatagt tacaatgata ctaaacttgg
     1081 acaaggaaga gaaaatgtta aaaaattctt ggaagataat ttagatttaa ctactgaaat
     1141 agatgagaaa gttagagcat tttacaattt aaatgaagaa catgaagaat caggtacttc
     1201 agtatcaaaa gaaattgtag aagaataagt aaaaatacaa aaaaataact agtaattaaa
     1261 gacatgccac gctccctatt agcttgacac aatgttgaaa aaattataaa attaagtgag
     1321 gataaaatat ctaaatttaa ttttaattat taggaatatt ttttaatcat agtgatgaca
     1381 ttttgggatt attaacgaag gaggtggatg tcatagatag tgtagtaatg attgtaatag
     1441 gtgcagctgt tggtattata gctggatatt ttgttagaaa aaatatatct gaagcaaaaa
     1501 ttggacaagc tgaaaacttg gccaaagaaa taatagataa agcacaccac gactcagaaa
     1561 cagtacaaaa agaaaaatta ctagaagcta aagaagagat ccataagtgg agaacagaag
     1621 ctgaaagaga aaacagagaa agaagaacag aagtacaaaa gtatgaaaaa agagtagtac
     1681 aaaaagaaga agtcttagat agaaaattac aaaatttaga atctaaagaa gtaaacctaa
     1741 gtgaaaaatt aaaggtagtg gaaaagaaag aagaagaagt cgaagtaata aaaactcaac
     1801 agttagaaaa attagaaagt atatcaggaa ttacttctga taaggccaaa gaaatcatct
     1861 taaccaatgc ggagagagat gtaagacgtg aaatgtctat aatgataaaa gagatagaaa
     1921 gtcaagctaa agaagaagct gataagaaat caagagaaat tataggatat gcaattcaaa
     1981 aatgtgcagc agatcacgta gccgaaacta ctgtaacagt agtaagcttg cctaatgatg
     2041 aaatgaaagg tagaataata ggtagggaag gtagaaatat aaggacttta gaaaccttaa
     2101 caggtataga ccttataata gatgacactc cagaagccgt aattttatca ggatttgatc
     2161 caataagaag agaaattgca agaattgctc tagaaaaatt aatagcagat ggaagaatac
     2221 atccagctag aattgaagaa atggttgaaa aggcgagaaa agaagttgac aatattataa
     2281 aagaatatgg tgaacaagct gcatttgaaa ctagtgtaca tgggattcat ccggaattaa
     2341 taagattatt aggtagactt aactatagaa ctagttatgg acaaaatgta cttaaacatt
     2401 ctatagaagt tgctcatata gctggtataa tggctgctga aataggagca gatattagat
     2461 tggctaagag ggcaggttta ttacatgata taggtaaggc tgtagaccat gaaatggaag
     2521 gtacacatgt tgaaataggt atggatttac ttagacgtta taaagagtct aaagaagtta
     2581 tacatgccat gagtactcat catggagatt acgaacctca aacagtagag gctgtattag
     2641 ttacagcagc agatgcaata tcagctgcta gacctggtgc gagaagagaa actttagaag
     2701 catatataaa gagacttgaa aaattagaag aaatagctaa ttcatatgaa ggtgtagaaa
     2761 aatcatttgc tatacaagct ggtagagaaa taagaataat ggtaaaacca gaagctataa
     2821 atgatgaaga aatacatcta ttggctagag atatgactaa gaaaatagag gatgaacttg
     2881 aatatcctgg acaaataaaa gtaagcataa taagagaaac aagagctata gagtatgcga
     2941 agtaaaagaa ataatatcta aataataaga aaatattttt agaaatttaa atttgccaca
     3001 agatatgata atatatcttg tggcttttaa attgtttaag tttaatttta tataatgcaa
     3061 aaataatcta agtgctaaaa gtgctactta caaaagatta ataaaataaa gctttatata
     3121 tgtattattt atataattat ccaagtttgt ttgatataat gaagttgtaa aatcaatact
     3181 ttactac
//
LOCUS       DAMJVX010000272         3129 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.279, whole genome shotgun sequence.
ACCESSION   DAMJVX010000272 DAMJVX010000000
VERSION     DAMJVX010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.279"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            81..623
                     /locus_tag="PC407_003539"
     CDS             81..623
                     /locus_tag="PC407_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2498944.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            637..3129
                     /locus_tag="PC407_003540"
     CDS             637..3129
                     /locus_tag="PC407_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2498945.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSINQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
BASE COUNT         1273 a          403 c          491 g          962 t
ORIGIN      
        1 tagcttacat attgattaat aaattttaaa ggagtataat aaacagtgtt cataataatt
       61 ttaataaaag aggtggtaat atgccaaaga ttttagaaaa tgtaaaagaa gatatattaa
      121 aagtatctcg ggatatgatt ttagaagaag attactcgaa tattagcata aggaaaatag
      181 ctcaaaggtg tggtataagt gcaggtacag tatataatta ctttaattca aaacaagaaa
      241 taatagaata tatcacaaaa tctgaatggg atttattgat aaggcgaatt gaatactcca
      301 ataaaaacac tgaagattac ataaaaaaac taaacattat ttttactgaa ataagaaatt
      361 ttataaacaa agttcataat atccaataca atgacttttt aaacactttt gaaacagaga
      421 gattttttga aatgaaaaaa cacaaagacg actttcatga acagctttca aataaagtat
      481 atgaagcttt agaaaaagaa agttttttta ataatgacaa attagtatgc aatatcataa
      541 taaaaatgtt tttttcatat tcaacatatc ttcatataga atttgaagat ttaaaaccct
      601 atattgaaaa attaatacat taaggtggtg tttaccttga atcataatac tagtattaag
      661 aacaaggaca gtaatccaaa aatattttta gtacttacac tttatctatt aggtatattt
      721 atgggagcta ttgatactgg aatagtatca ccagcaagaa cgattataca aaatagcctt
      781 ggagtaaatg aaaaaacggg aatttggatg attaccatat acacattaac atacgcttct
      841 gtaattccta tctctggaaa attagcagat aaaataggaa gaaaatatgt gtatctagtt
      901 agtatatttc tatttggtag tggttcatta atatgtggat tatcatcact attttccaat
      961 ttctatattt tattaattgg tcgtgttatt caagcaattg gtggtggtgg aatcatgcca
     1021 attgctacag cagaatttgg aactaccttc ccagaaaata aaagaggcat ggctttaggt
     1081 ttagttgggg caacatatgg tattgccaat attttaggtt caagcattgg tagcacaatt
     1141 ctaagtatct ttggaactca aaattggaag tggctatttt ttgtaaacct tccaatttgt
     1201 ttaatcatac ttattggtgg agttttctgt attaaaaaca ataagtctga atctaaagaa
     1261 aaaatagata agcttggtac attgatgttg atatgtataa tcgtatcact attatatggt
     1321 cttatgaata tagacttttt taattttaaa aattctatac aagatatatc agtttatcca
     1381 tacttattga catttattgt acttatacct attttcatat ttattgaaaa tagagctaaa
     1441 gaccctattt taagctttga atatttttta aatccgagaa tattgatagt acttatttta
     1501 tctttaattg ttggtattgg aatgatgggt atggtattcg ttccacagta tgctgaaaat
     1561 gcattaaaga taaatgctgg ttctggtgga tattttgtag ctatattggg attatttgca
     1621 ggtattgctg ctcctttaag cggaagactt attgacaagt ttggggcaaa gaaaatacta
     1681 ttactaggat tttctgtatc tatgataggc tctctttacc tagttttaat tgctctaaaa
     1741 accaatactg tatttagtgt atgtgtaagt ttaatattta tgggattagg aatgggcttt
     1801 actatgggaa ctcctctaaa ctatatgatg ttatcaaata caagattaga agaatctaat
     1861 tcagcacttg caacattatc cttgatacgc tcaattggta cttcaatttc tccagctata
     1921 atgattggat ttatagcaca tgcaggatta tctgtgcaag ataatattat ggacttagtt
     1981 ggaaagccct caactcctaa aatagttcaa cttgaagaac ttgaaaacat gatagatgac
     2041 ttaaaatcag accctgaaat ggcaaaaaat ttaaaaaatg tctctatccc aaatatgaat
     2101 gatacttcaa atataaatat ggacatgaca ggtggcaaac ttccaaaatc cttacttaat
     2161 aaagttcagt cagcagatac aaccaacatt accaatataa caaaagaaat atcaacaaca
     2221 atgtttgatg aaaaagttcc atctataatt actaaaatac agaaaaatgt acaaaaagga
     2281 atagatggaa cacaaagtgg aataaacggt attgaagatg gtgaaaataa actaaatagt
     2341 ggtattaaag gagttcaaac tggtattgac aatatggtaa aagctagagc tggtattaat
     2401 caaggaatag atggtgtaaa aaaaggtatt gctggtgttg acaaaggtat aaaaggtatg
     2461 gaacaaggtc ttgaaaaaca agatgaggcc atcaaggaac ttacaactgc ttataataaa
     2521 atacctacta cagaaaagac tcctactgat aacgaaaata actcaaatga acaaaaccag
     2581 agtaatacca gtaattctga atcagacgac actaagaatg attctacaga aaataagaat
     2641 aacaattcca gcgagaataa accaaatgta aatgaacaaa aagaaagttt aaatgctcaa
     2701 atacagcaac taaaaaagac aagagatgac ttaaatacaa agttacaaaa aaataaaact
     2761 caaaagaacg aattaagtaa aaaactaaga tcaatggaat ctcaaaagaa agagctacaa
     2821 agcaagttaa ataattctat aaaccaaaag aaagacatgg aaaaaagcct tgatacaatg
     2881 caacagcaaa aaaaatcact ccaagcagtt ttagcaaaga cacaagaggt aaaaactgaa
     2941 ataccaaagg cttttgataa atcaaaattg gattatataa attccataga aaataataga
     3001 acaaaaatag aaaatacact acaatctact ctaaactcag gattcaaaca gatgtatata
     3061 acagtattct gtgtaaatct actagcattt ataatattat tgttctataa agaaaataaa
     3121 actagataa
//
LOCUS       DAMJVX010000273         3010 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.280, whole genome shotgun sequence.
ACCESSION   DAMJVX010000273 DAMJVX010000000
VERSION     DAMJVX010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.280"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            132..500
                     /locus_tag="PC407_003541"
     CDS             132..500
                     /locus_tag="PC407_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2498946.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            503..874
                     /locus_tag="PC407_003542"
     CDS             503..874
                     /locus_tag="PC407_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HDF2498947.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(1018..1473)
                     /locus_tag="PC407_003543"
     CDS             complement(1018..1473)
                     /locus_tag="PC407_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498948.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1512..2777)
                     /locus_tag="PC407_003544"
     CDS             complement(1512..2777)
                     /locus_tag="PC407_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HDF2498949.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         1119 a          414 c          414 g         1063 t
ORIGIN      
        1 aattcaaagt tatattagta gaaattggaa ttatttataa tatactccaa aatttaaaaa
       61 taatggttga aaaatctaat taagagtact ataatttaat taagtaatta cttaatcaaa
      121 acgaggtact tatggaaaac ttggttaaaa tatttaaagc attgagtgat gaaactagac
      181 ttaacatact tatattagta tccaagagaa atatatgtca aaaggggatt tctaaatatc
      241 tagggatttc agattctgct gtttcacaac atataaaaat attaaaagat gttgggataa
      301 taacaggata taaagaagga tactatgttt tatatcatat taataaagaa tcatttaata
      361 tatgtgtaga ttttataaat tctatgttaa gtaattctag tgaaagcttt atagatgtat
      421 ttgatgtaaa tacaatacat ctaggatgca gtaaagaatg taaatctata aaaaaatgtt
      481 gtaagagaag ggagaaataa taatgaaagt atgtatacca gtagaggaga ataaaggatt
      541 ggatagtaaa ccatatggac attttggttc tgctccaatt tttgttgtgt gtgatttaga
      601 aagtggagaa gttaaatctt tagataatgg agatttagat catgaacatg gaaaatgtca
      661 accattaaaa gcattgtcag ggacagctgt agatgcagta gttgttggag gaataggaca
      721 aggagctata attaaattaa atggaatggg aataaaagtt tacagagcag aaggtgatac
      781 tataagtgct aatttagact tacttaaaaa tggaaaatta gtagagtttc catcagacca
      841 tacttgctca catgacggat gtggacatca ttaaaaaaaa cagctgatga aatatatact
      901 tcatcagcta ttttttcttt taataaatac gattttattc ttttaaaacc aaaatttaga
      961 tttttatgtt ttttgtagta tccttaataa tatttcaagt ttacaagttt actactttta
     1021 gatttttttt gtatgttttt ctatatagtc aatatcattt aatatgtcat tgagttcttt
     1081 attaagtgtt tttaaatcat caccatctac attgtagttt aaaaatagtt cctctacatt
     1141 atcaatgata gaattgactt ttctagtaac ttcaacaaat gtcttaaagt tactactatt
     1201 tttagcattt agaaattttt ttaaatcctt tgatataaat gatactattt catattcatc
     1261 attaaataga cctctaccaa taggatatgg ttcaattctc tgtaaaaaat attggtcttt
     1321 agcattaata ttataagtat aagatatttc ccaaccgtct cctaaatcaa acttgataaa
     1381 atataatgta ctacttactt ttgatattct tgtggcacca agctgtctta aaagaacttc
     1441 aatcgaatca ccttgaacag tattaatatt catatataca catccccttt agtattatta
     1501 tactatattt tttaaaattt tataattaaa taaacagtag atataataat agatataatc
     1561 ataagtggaa aacctatttt gaaatactcc ttaaaagaga taggatgacc gtgtttatta
     1621 cctatacctg ctagtacaac attagcagat gctcctatta gtgttccatt tcctccaaga
     1681 caagctccaa gagatgttgc ccaccatagt ggcattacat caataccttc agcttgcata
     1741 gtcaaaataa gaggaattag tgttgcaaca aatggaatat tatctaagaa agaagagact
     1801 atagctgata accataatat caacaacatt gtaaatacaa gatgtccttc tgtaagtcca
     1861 atcaatgctt gagccaattt gtttattatc ccaacttcta ctagtccacc aactactaca
     1921 aataatccca taaagaataa aattgtggac cattcaatac ctgccataat ttcatctaca
     1981 tcttgttttc ctattaaaag cattatagat gctcctgtca gtgctacaac agaagaatct
     2041 atgtggattg tactgtgaaa cataaaacca aaaagtatga atgctattaa tataaggctt
     2101 tttataagta gaggtttatc tttaacagat tttttttcat ctagttttaa tattgcattt
     2161 ttagctcttt cattcacaac aagttctttt ccataaataa atctaaagca tattatagtt
     2221 atagcaagta ttactataac agcaggacct aaatttatta caaaatccat aaaactaaga
     2281 ttggcagcac taccaatcat aatatttgga gggtctccta taagagtagc tgttcctcct
     2341 atatttgaag ctaaaatctg tgttattaaa aatggaactg gattaagtcc taatatttgt
     2401 gtaataacta tagtcattgg tccaataagt aagacggttg taacattatc taatacagct
     2461 gataaaatag ctgttataat agcaaaacaa atcattatct tccatgggtc acctttagct
     2521 ttctttgcag tccagatagc tatatattca aataaccctg agtttttgac aacagctaca
     2581 aatagcatca taccaactaa tacaccaata gtattgaaat ctatatggct taatccttca
     2641 tcaagtgtaa gtatattgaa tattatcatt aatagtgcac caccaacagc tgctacggat
     2701 ctgtttacct tttctgtaat aatagctgcc ataactatta aaaatataca tatagctatt
     2761 atttgttgaa attccataat ataagtacct ccatttgtat tattttgtgc ctaatgctag
     2821 tactatatat agtattactt ttttattgtt ttttcaatgg atgtatgaaa gtatattatt
     2881 taacaaagtt tttgagaaat tgacagaatt taaagaaaat ttttgttatt taaataaatt
     2941 ttaaaattat tccattgata ataatattaa acgacaaacg aaaaaatata ccctttaaac
     3001 tagaatttat
//
LOCUS       DAMJVX010000274         2986 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.281, whole genome shotgun sequence.
ACCESSION   DAMJVX010000274 DAMJVX010000000
VERSION     DAMJVX010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.281"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            70..2427
                     /locus_tag="PC407_003545"
     CDS             70..2427
                     /locus_tag="PC407_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDF2498950.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKDDFLYSGSFVVSGEAFVRVENIGKDNYANKIVNDAKISKKHNSQLRNSLN
                     SILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKIRIEVEKYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDNDIYEAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2513..2830)
                     /locus_tag="PC407_003546"
     CDS             complement(2513..2830)
                     /locus_tag="PC407_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDF2498951.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         1144 a          330 c          477 g         1035 t
ORIGIN      
        1 aaattgagaa ttttataata tatttgtaga tttgagtata aaaaagtgct aagttgaaag
       61 gagtaaaata tggataaggt taatttagaa aggtatagtt gtgatataaa tgttggttta
      121 aataaaaatc aacttcaaag tagaatagat gataatttaa ttaatgcatt tgataatgga
      181 aaaaccaaaa catataaaca aatttttaaa gacaatatat ttacactttt taatctcata
      241 aatattgttt tagtctgttt attagtttca gttggttctt ttaaaaatac cttatttata
      301 tttattgctg taattaatac tattatagga gtaatacaag agataagagc taaaagatta
      361 ttggacaatc tatctgttat agtttcaaat aaggtatctg tcataagaga aaaagagaaa
      421 aaagagattg atgtacaaga attagtatta gatgatatta tgattttaaa aacaggtaat
      481 caaatttgtg cagattcaaa agttttaaat ggaaaattag aggtaaatga gtctttggtt
      541 acaggtgaat ctgatattat tataaaaaat aaagacgatt ttttatattc tggaagtttt
      601 gtagtttctg gagaagcttt tgtaagagtt gaaaatatag gaaaagataa ctatgcaaat
      661 aagatagtta atgatgcaaa gatttctaaa aaacataatt ctcaacttag aaattcatta
      721 aattcaatat taaaaattgt aggtataata ataattcctt taggaattat attgtttgca
      781 aaacagcatt ttggaaatgg agattctata gctacatctg tagtaggtac agtggcagct
      841 gtaaatggta tgataccaga gggattaaca ttacttacga gcattgctct agctgtagga
      901 actataaaac ttgctagtca taaaacttta gtgcaagaac tgtactgtgt agagactctt
      961 gctagagttg atacactatg tcttgataag acaggtacaa ttacagaagg taaaatgcaa
     1021 gttgatgata tagtaatgtt ggaagaagtg gatattaatg aaataatggg gaatatatgt
     1081 aattctctca aagatgataa tgcaacacta aatgcaataa gagataagta taatgtttta
     1141 gatacgtata aagctaagaa tttaatacca ttttcttcag aaagaaaata cagtggggtt
     1201 acctttgaaa ataaaggtac atatttatta ggtgcatttg agtttatatt taaggaaaaa
     1261 aataaaaaaa ttagaataga agttgaaaaa tattctaaaa aaggaaacag agtaatagtt
     1321 ttagctcata gtgattctta tatggaagat aataatattc ctgaagatat aaagccacta
     1381 gcttttatat tgatattaga taaaattaga gatgaagcta aggaaacttt agaatttttt
     1441 tacaatgaag gtgtagatat aaaaattata tctggtgaca atcctataac agtaagtgag
     1501 gtagctagaa aagcaggtct taaaacggct agtaatttta ttgatgcaac aacacttaaa
     1561 aatgataacg atatatacga agcagttgat aaatacagtg tttttggaag ggtaagccca
     1621 caacagaaaa aacaaatgat tttagcatta aaaaaacaaa atcatacagt agctatgact
     1681 ggagatggtg taaatgatgt gctagcgctt aaagaagcag attgcagcat tgccatggca
     1741 tctggaagtg atgttactaa gaatgtttct aatttagtct tattagattc aaactttgca
     1801 tcaatgccaa aggttgttat ggaaggacga aaggtaatta ataacattca aagagcatct
     1861 tctttgtttt tagtaaagac aattttttca ttcttcttgt cactattaac attattcttc
     1921 ttcagtagac agtatccatt tgttcctata caattatcac taattggagc tgtaacagtg
     1981 ggtataccat cattcttctt agctcttgaa gcaaacaaaa gtagaatttc aggaaacttt
     2041 ttactcaata tattaaaaaa tgctttacca ggagcactat gtgtgacagt aaatgtaatt
     2101 attgtttaca atatagttga atatttaggc tatagttcaa gtgtattttc tacaatgtgt
     2161 gctatattaa ctggtgtatg tggtctttta atattaagac aacaatgttt gccatttaat
     2221 aaagaaagat tatttttaat tgtaagtatg actattgcat ttgttattgg gattctattt
     2281 ttaggtggat tattctcatt tgtagcattg agtagagaat taattctcat aactatggca
     2341 ttagcaatat taatgccaat tatgataaaa gttatgttat ttgtcttgga tgagatatta
     2401 gagacaattg ttccagattt aaattaattt taaaatgata taaataagag cagttttagt
     2461 tgaactgctc tttttagatg tgtttaattt tatataagaa tatgtatttt tattaatcag
     2521 ctatatatga gcaacagtag tcaacaactt cattaacctt taaatcaaaa cttgaatcac
     2581 taggaacatt aaatttttgc ccttctttat aagttacaaa ctcattgctt ccaggcagtt
     2641 ttacatccat acttccagca agcatctcca ttatttcttc ttctctagta gaaaaagtat
     2701 attctcctgg caacattaat cctaaagttt ttctttctcc attaggcagt attattactc
     2761 tgctacttac cttaccatca aaataaacgt ttgccttctt tactgcagta acattcttaa
     2821 attcactcat tagataaaac ctccaaaatt atttgtacat atatattata taaaaataat
     2881 gtaaaaaatg ctatatattt gcaaaaaaaa tttagtattt attaaaaaaa ttaataagaa
     2941 taaaaaagag atgaaattgc ttaaattgat aaaaaatata aaataa
//
LOCUS       DAMJVX010000275         2979 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.282, whole genome shotgun sequence.
ACCESSION   DAMJVX010000275 DAMJVX010000000
VERSION     DAMJVX010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.282"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            94..600
                     /locus_tag="PC407_003547"
     CDS             94..600
                     /locus_tag="PC407_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDF2498952.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            728..1744
                     /locus_tag="PC407_003548"
     CDS             728..1744
                     /locus_tag="PC407_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2498953.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2098..2802
                     /locus_tag="PC407_003549"
     CDS             2098..2802
                     /locus_tag="PC407_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HDF2498954.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVIICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         1258 a          282 c          523 g          916 t
ORIGIN      
        1 aatcttccta tatcatataa aattaagtat tgattagtct attacataaa tatagaatat
       61 aaatatatcc atttaataat aaggagaaga gatatgaaag aatatgtagt tgtggattta
      121 gaaactacag gattagatcc atataaaggt tgtgaaataa tagaaatagg aataacagaa
      181 ataaaaaatg aacaaattgt aaaaaactat tcaaggttga taaaacctaa ggggataatt
      241 tcttcattta taacagaatt gacacatata agtaatgaaa tggtagaaaa tgaagaacca
      301 ctagaattag ttttacctag atttagaaaa tacataggtg atagaactat aattgcacac
      361 aatgcgaaat ttgatttaaa atttttaaat tactatctta ggatgttaga tttagaacca
      421 ataaacaatt atatatgtac agtagaatta ttaaaaaaat gtaaaagtta taaaggaaaa
      481 aacaagaaac ttgaaacagc ttgtgcttat tacaacatag agaatataaa tgctcataga
      541 gctgacagtg atacattagc aactgcaaaa ctatttttga aaataaaaga tgaatattaa
      601 ttataaatat agagaaaatt agtgaaaata tcctttataa aattgaccct taagcttaca
      661 attttgttct ttgatttatt ttgtagaaca atgtataatg taccttatat gaagaaggga
      721 gactaaaatg ggttattaca atgataatga taataataaa ataagacgta aaaaaattag
      781 taacaacaaa gatagtaata aaaaatctaa acattcttat acttcggcaa ataaaaaaaa
      841 taaaagtcct aatacaagtt ccagtaaaag aatgagtgaa aaagtagatt cacagaatct
      901 tataaaaaga gatagagata gttttacaag agataatgta agacctttaa atacagatta
      961 caatcaaaga gaaagaagaa gagtcgagta taaacaaaga gagaaaaaat taaaaagtaa
     1021 aaaaagaaaa aaacaaaaaa ggattagaat agttagggca ttattatcta ttatgttagt
     1081 tgcactaatt atattttcag tggcaggtgc aatatttaca gtttcagcta taaaagggtc
     1141 accagaagtt actaaaaagt taattaggtc taaatatata agtagtgagg ttgtaagtat
     1201 aaaacaaatg ccaaatgatt taaaacatgc aatagtttcc atagaagatg agagatttta
     1261 taagcataaa ggagtagatg ttatttctct agttaggtca gtattaaaca atgtgtttac
     1321 agatacaaca caaggtgcaa gtactataga tatgcaagtt tcaaaaaacc tgcttactag
     1381 taatgataaa actatgaagc ggaaaataag ggatatgtat aatgctaagc agatgaataa
     1441 gattatgact aaggatgaaa ttcttgaggc atatttaaat aatatgtatc taggtaaaaa
     1501 tgcatatgga gctgcaaagg gagctgaagt ctactttggt aaaaaagtta gtgaattaaa
     1561 tttagctgag tgtgctatgt tagctggtat gacaaataat cctgcaaggt atattgacca
     1621 tggagaggct aagaagagac aagtgattgt actgtataag atgcatcagc ttggttatat
     1681 aactgataag gagtatagaa tagcaaaagc tgatgacact ccatttaaat cagaaattga
     1741 ttaaaattgt aagaatatag tattttcaat agtttaaaca aacaaaaatg taataaaaaa
     1801 ggtgatttgc cacccatttg ccaccataat tataatctaa tggcaaattg ttttaatgta
     1861 caaactagat aatagaatat tgttttaaat gctatattat gctaaaattt aattgtattt
     1921 tgtaaattaa aaaacttata atttagtttt ctagggttcc gcattttata atggtcaggt
     1981 ccaagagaaa acgcgcaatt gttgtgtaca cggaaggata aaagcctggg agatatttaa
     2041 taatatttct ataggctttt ttatttttac atattttaaa tttgaggagg ttttctaatg
     2101 aaaaaatttg ttattgagga tgcattttgg aatctatttc ctagtgcaaa aattggagtt
     2161 attatttgta atgatataga taactctata aaagatgaag actataagaa aataattttg
     2221 aagggagaag aagaagcatt aaaatacttg gaggactcag aatttagcaa caaccaagtg
     2281 attaaagtat ggagagaggc atttaaaaaa tttaaaacga aaaaaggagc aaggtcttct
     2341 attgaagcat tattaaaaag agttcataat ggaaataata taggaactat taacccttta
     2401 gtggatatct acaattttat ctcattaaaa tatgcattac cttgtggagg ggaggatata
     2461 gataaattta taggagatat taggctaact agagcaattg gtaatgagga gtttatccca
     2521 ctagggacag atgaaaattc atttccatat gaaggagaaa taatttataa agatgatggt
     2581 ggagcaattt gtagatgttg gaattggcgt gaagcagtga gaacaatgct tactgaagat
     2641 actaacaatg cctttttatg cattgaatta gtagatgaaa gtagatttaa agaatttgaa
     2701 aatgctttaa atgaattagc taaaatagtt caagaaaaat tgggtgggaa ttgtaaaatt
     2761 tcaattttag atattgataa taaagaggta tcagtggatt aatctggaga tgctgttgta
     2821 atggactatt ctagttataa agatacttat atttagggta ataagttcta ttttagatgt
     2881 aacgattatc aaaggatata atatagaaaa taaaaatgta gaatataaaa agcctttaaa
     2941 ataagaaaaa aatgtatttt aaaggctttt attatactc
//
LOCUS       DAMJVX010000276         2962 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.283, whole genome shotgun sequence.
ACCESSION   DAMJVX010000276 DAMJVX010000000
VERSION     DAMJVX010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.283"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            203..1387
                     /locus_tag="PC407_003550"
     CDS             203..1387
                     /locus_tag="PC407_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498955.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(1764..2873)
                     /locus_tag="PC407_003551"
     CDS             complement(1764..2873)
                     /locus_tag="PC407_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2498956.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
BASE COUNT          983 a          407 c          447 g         1125 t
ORIGIN      
        1 aaattaaaaa acaaagtgaa tttgaatact atttaaaaaa taaaaaaata tataaaaaat
       61 atttaataca aaggtagaaa taagttgaaa atgtaaagtc attggaaaaa tatagttgaa
      121 tttaatgaac aaaaatgtta ttgttttatc aatgaagtgt taaaataaaa actttcaata
      181 tcattttagg gaggatgatt tcatgcaaaa aagtgtaaag aaagcaaaag taacgggatc
      241 tatgttagcc atttttggtg ttgcaagcgt tctttttagt tcacatgcag gaggaggatt
      301 tgctacaggg aatcaagaaa ctcagtacta tgtgcagtat ggatggacag caccacttat
      361 ggcaatttta gcaatgatta tacttactgc taccatgaga gaagttatca taatgtataa
      421 taataacaat tgtaggaatt ataaggattt attttgtgag ctttggagac catatccaaa
      481 acttgaaata atatgggaga tatattacta tctaatggtt ttaatagctg taagtgctgt
      541 tatagcaggt gcagcagcag tatttcaaag tataggagta aattattttg tagctgtatt
      601 tattattgga gttgtacttc ttgtctttac tatatttggt gctatgcttg tatctaaagc
      661 agctacagca atgacaatag ctattctagt atgtactctt actattttta ttgttggtat
      721 aaaagctaaa gtaccagaaa taactgaaat attatcaaat agaacgagtt tcacacctgg
      781 ctatatcaag ccaatactta atacttttat ttatgcaggt tttcaatcgg ttgttatacc
      841 tacacttgca ggatgttcaa gaccacttct taagaactca aaagaagcta ctaaggcaat
      901 gataatttca tttgttatga atgcaattgc attaggactt gctgtaacta tgctaatggg
      961 atggtataga gaaattattg ctgctgggca aacaacactc cctactcttt atgttgcagg
     1021 tcaatctgga aatcatacta tatacataat ttataatgtt gcattattcc tttgtttaat
     1081 gtctacagga gtaactacta tatttggtct tgtaaataga tttgaagacc ataaagcact
     1141 ttctttcctt tcttcaagaa tgaaaagaag ggtatttaca gcttgtgcaa taatggtagt
     1201 gtcaatgctt atatcactta caggattaag taatattgtt aaatatggat atggatattg
     1261 tggttatctt ggtctattca ctatagtaat tccattcctt acactaggac attataagaa
     1321 taagaagttt gcaaaggaaa atccagaagc taaatggcca gcggaattaa atgaaaatgt
     1381 aaattagatg tagtagcaaa aaatattgct aaagagaata ctataaataa gaatactata
     1441 gataatatat ctgcatgttt attaagatta tttgcaaaat ctaatattga acaactataa
     1501 acaagcaata taaaactaag aataaaagat aaaaagaggg tgtctcaaaa tggactttta
     1561 gtttataagg cactcttttt tatatgaagt caaatatatt ttcattattt tgacaataaa
     1621 agagactaat cctactttgg atagcctctt ttttctttaa tataaaatag tataacagca
     1681 aagtatagat tgatacatgt tttctgtagc tttctatata ttacagtgtt tgtgtggcat
     1741 tggtatttta agcaatctat aaattataat tctataattc ttctactaat tttagattct
     1801 atagccgtct ctatttcact taacattcgt tcatttttag ggtcaataaa agcacatgta
     1861 tagccttctt caccagctct accagttcta ccaatacggt ggatgtagtc ttctggtgtt
     1921 tctggtaaat cgtaattata tatatgactt actccagata tatcaaggcc acgagaagct
     1981 acatctgtag ctattaagta ttgtatatct aaatttctaa atgatttcat tattctttct
     2041 cgttttgatt gagcaatatc actatgaagt ttttgacaat tatatcctct tatagcaaga
     2101 gcttcttcaa gattgtcaac ccttctctta gtcctacaaa atattattgc cataaaagga
     2161 ttatcttcat ctagtacctt acataaggca tctaattttt ttctatctgt agtttctact
     2221 acttcttgtt ttatattatt aagagtgatt tcttcttttt gaatagaaac tacaacagga
     2281 ttttttgtgt atctataagc taattttttt acttgagaat ttatagttgc agaaaagcat
     2341 agtgtttgct ttttgtttga agtctctttt aaaatagctt ccacttcgtt tttaaatccc
     2401 ataagcaaca tttggtctgc ttcatctaaa acaaaagtct ttaacttact aaggtctatt
     2461 gtttttctat ttagatggtc taaaagccta cctggtgtag ctataatcat atgaatattt
     2521 ccttttagtt tctttatttg agaaccaata tcttttcctc catatgctgc aagaatactt
     2581 acatctttag attctttaag tttcatagct tcttctgtta tttgaatagc tagttctctt
     2641 gtaggagaaa gtataagtac ttgtatatca tttatatcta atgatatatt ttcaaatatc
     2701 ggaagtaaaa aagctagtgt ttttcctgtt ccagtttgag cttctgctat tacatctttt
     2761 ccttctttta ttagtttaat actttctttt tgtataggtg taggtgttgt tattcctgat
     2821 ttttttagaa tatttattgt attttcacta atccctaagc ttttgaaatc cattgttatc
     2881 ttacctttca tgaaaaattt tcttttttat taaccaatta ataatatcat atttgtaagt
     2941 atttttagta aattttttaa tt
//
LOCUS       DAMJVX010000277         2915 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.284, whole genome shotgun sequence.
ACCESSION   DAMJVX010000277 DAMJVX010000000
VERSION     DAMJVX010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.284"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            391..789
                     /locus_tag="PC407_003552"
     CDS             391..789
                     /locus_tag="PC407_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2498957.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMMILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(1006..1215)
                     /locus_tag="PC407_003553"
     CDS             complement(1006..1215)
                     /locus_tag="PC407_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498958.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(1247..1456)
                     /locus_tag="PC407_003554"
     CDS             complement(1247..1456)
                     /locus_tag="PC407_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2498959.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            1755..2174
                     /locus_tag="PC407_003555"
     CDS             1755..2174
                     /locus_tag="PC407_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2498960.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            2402..2857
                     /locus_tag="PC407_003556"
     CDS             2402..2857
                     /locus_tag="PC407_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498961.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
BASE COUNT         1111 a          356 c          355 g         1093 t
ORIGIN      
        1 atcttattct taaatatttt accctaaaga ataaattcat aaaatctttg taattgatta
       61 atataaatta caatataata ctatttttat cttaatcttt ttctctttta cagacaatca
      121 aaaagcaatc ttagtttatc gaaataaatt tatacaatct attcatacac ccctttacat
      181 ttttataaca attaaatcta ttgttatata tacaaatcaa aataaaccat atattttcaa
      241 ctataatatg ttaaaatcat attatgagca tattaattta agcaaaatgt catttagact
      301 ttaatttagt agttttatat tataaattta tatattttta atttcaaatg tcatttagac
      361 attactcata aaaagaaagg agtgtatttt gtggatactt taggaaaaag aattgcttat
      421 ttgagaaact ccaaaaaact tactcagcgc aaacttatgg atattttaaa atttgaaaac
      481 ttaggcaagt atgaaacagg agatagaaaa cctaattgtg atatactaat gtcaatagct
      541 gattatttta atgttacgac tgactggctt ctctatggta aagagaaggt aaatgtcaat
      601 agtagtgtaa aagaagataa agaagattac ttacatgtta ctaatgatga aatgatgata
      661 ttaaatctat acagacaact aaacgaacgt gataaaataa aaatagaagg aatacttgaa
      721 cttaagatat ctgaatataa agacttaaaa aagcattcct ctaataataa tgaagataaa
      781 atggtttaaa catacataat ttgatacgat tttacatcat ctattaacct tctttttttg
      841 tagtatatta ctaaaatatg gatattggat tatttaaaaa caagtatagt ctaatttcaa
      901 gcacatttat aatatttaga ttatcttatc catattttag tttaagtact ttgaaaaata
      961 taatacattt ctatgtataa tttatattcc taagaaatgc attactcatg tttttaattc
     1021 ttccaacatc tttctataca caattgtaca tccttcataa ttcccctctt taagaaggat
     1081 tgaacatgta ctagaatatt tagttaaagt atttaatctt tccatattta agctttttat
     1141 attactaagt gctgtaataa aatcatcaat tgctctacaa cactctataa ccccatctga
     1201 atccaatttg cacacaagaa tattcaccta ctttatatac tatttattaa tcatcttctg
     1261 aatattctat attaagtaat tgataaattg atgatttata ttttttacct gttcttcttc
     1321 catttaaaat agctgacaaa tagttttcat taacacctat tttcttcgct aattcccttt
     1381 gagtcatatt taattcaata agtctttgct tgacttgcac tcccaaaaaa ggtatccatt
     1441 tttttcgagc atccatttat caacttcctt tctaagtgat ttatatttac ttttatatta
     1501 ctttagctta cgcaccccct ctatttagta tatattttta atatgtacac atattaagta
     1561 tataaattta tttaatacta aataagattt tttgattact ttgtgctaaa attaggatgt
     1621 aaattcgtat cattaagtta attataaagt cattttgacc ttaagtcaat ggtttttatg
     1681 tcattttgac ctttaatttg aaactattta catcttaaaa agtctttttg acattaattg
     1741 aatggagagt gtttatggat actttaggag aaagaattgt ttatttgaga aaagcaaaaa
     1801 accttaaaca atatgaacta gaagaaatgt taggctgtga caacttaagt aaatttgaga
     1861 gaaacattag aaaaccaaat tatgaaatat taaaatctat agctgagata tttaatgttt
     1921 cagtagactg gctattaaat ggagataatc tatcacataa atcagattta atttgcgatt
     1981 cctcctcaaa ctatccactt aatagcataa actctaatga aataaagctt ttaaacaact
     2041 ttagaaagtt aaatgattat gataaagcca aaatagaagg tatgattgaa ttaaaacttc
     2101 atgaatatga aaaagagaag gatttaggaa aaattgagta taacaaaaat aaagatgaga
     2161 aaatagataa ataattttta tgttgctttt gatacgattt tacatcattt ctttgttcta
     2221 atatttttat ttcatttatt ttctttttta taattttatg ataaaataat tagtatattt
     2281 tgctttactt gaatgtaatt atttatacaa aagtcaaaca taatataaat gtagtaaatt
     2341 tttagtttca agtaatatct cgttattata actaaatatt tattaaaatt aggaggataa
     2401 aatgggctta agagacaaat tcgcgcaatc atttgctaga tcaaaaacta tgagtggccc
     2461 tgagaaaaag gctaatgaaa taatgggtaa attgcttctt aagaaagcta tattacctat
     2521 cgttttaatg ttcgtgataa taatcgctgg agctatgctt aaaatcaatt cttgggttac
     2581 acttggcata aatttagtta ttgctgttgg tgctttcttc tacataagaa attctagtaa
     2641 aaagtatcag aactttaaac cttatgttgg caatttgatt agtttagaga aaaaaggtaa
     2701 aaaagagtac gttgctatta taaaacaagg taagttacca gttaagttac aaatagctta
     2761 tggtggtgaa gaccttgaac atgttaagaa aaatcaaatg gttcagataa gttataatcc
     2821 agatgctaaa atagctattt tggttaatag acaataattt aataaaaaaa aggagttgtc
     2881 ttaaaattga ttttttctaa tcaaatttaa gctta
//
LOCUS       DAMJVX010000278         2911 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.285, whole genome shotgun sequence.
ACCESSION   DAMJVX010000278 DAMJVX010000000
VERSION     DAMJVX010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.285"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            323..1600
                     /gene="hflX"
                     /locus_tag="PC407_003557"
     CDS             323..1600
                     /gene="hflX"
                     /locus_tag="PC407_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2498962.1"
                     /translation="MDMTKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELYDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDCVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            1833..2330
                     /locus_tag="PC407_003558"
     CDS             1833..2330
                     /locus_tag="PC407_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2498963.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            2380..2904
                     /locus_tag="PC407_003559"
     CDS             2380..2904
                     /locus_tag="PC407_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2498964.1"
                     /translation="MKSVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKDLQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKVPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILNYIRKL"
BASE COUNT         1204 a          263 c          520 g          924 t
ORIGIN      
        1 catataagtt aattaaacta atgtattttt aatatatatt tagaatttta atccttctta
       61 tgatataagt agcttgtttt gtattactta gatttgtaag gagttttttt attgaaaaaa
      121 ctgcataata tatttgggag gaggtgtaag aaagtgattt tttatataag attgagaaaa
      181 taatgcacga ttatggttat tataatagta aataagaagt gtcatttaag gataagataa
      241 aaagcaaaat tcttgatgaa tttaattata gagccataat ctcgtgctat agattaaata
      301 aaaaatagca gataaggaga atatggatat gacgaaaaaa ggaataacag taggattaaa
      361 cataaataat aaatctgagg actttaatga actaatgatt gaacttgaaa acttatgttc
      421 agcatgtgat attgatgttg tagggagtat aactcaaaat gcaaagcagg ttaatagagc
      481 attttacatt gggacaggta aagttgagga aatattaaac ttaataaaga aagaaaacat
      541 agagatagtt atattttaca atgaactttc tacatcacaa ctaaaaaatc ttgaagaaaa
      601 gcttaattgt gaaattatag atagaacggc attgatactt gatatatttg cacaaagggc
      661 aaaaactaga gaagctaaac ttcaagtaga agtagcaagt ttaaaatata tgttaccaag
      721 acttataggt tcaaatgaaa atttaggacg tcaaagtggt ggagttggta ctaaaaatag
      781 aggttctggt gagaaaaaat tagaacttga ccgtagaaga atagaagaaa agataacatc
      841 tttaaatagg gagttgtatg atttaaaatt ccaaagagaa actcaaagaa gtatgagaag
      901 aaaatcaaat cttccaaatg tagctctagt cgggtataca aatgctggta aatcttcaat
      961 aatgaataaa ctagtagata tatttaaaaa ctctgaagaa aagaaagtgt ttgagaaaaa
     1021 catgttattt gctacacttg aaacttctgt cagaaatata gttcttgcaa ataacaaaga
     1081 atttttatta tctgatactg ttggttttgt aagtaatcta ccacatgact tagtaaaagc
     1141 atttaggtca acattagaag aagcatgtga ggcagacgta ctgttacatg ttattgatat
     1201 ttcaaatcca agctataaaa gtcatattaa agtgacagaa gatactttaa aacaaatagg
     1261 tgcagatggt atacctatga tacatgtgta taataaaatt gatttaatag atgtagaagt
     1321 attggataga attttagata gtatcgataa agaaggtata tttgtttcag ttaaaaaaga
     1381 tataaatata gataaaatga taaaatgtat ttgtgatagc atatttaaag attgtgtaag
     1441 atgtaagttc ttaattccat atgataaagg tcatgtagtt tcttatttta acgaaaatac
     1501 gagcattata aatacagaat acagagaaga tggagctatt ttagatgtag aatgtagcaa
     1561 tatagaatat aataagtata aaaagtatgc tttagagtaa attttgaaaa acttattata
     1621 gcctaaaata tatttaattt ataatttatt ataaattgat tgtaaatatg attttatgaa
     1681 acggtatagc cataatgcta tgccgttttg ccatttataa taaaaataaa tttattgaca
     1741 caatagtaac taattactat aatataaagt aactagttac tatttaaaat tatcaaaaaa
     1801 ttaaaatcta agataaagag aagtgtataa taatggatgc agaaagttta atgaaaaaat
     1861 atatggtaga tgatgatagt agaaaacaag ctatatttgc aagtatattt gtaatgcaaa
     1921 ataaacttca aactacatgt gataaattag acccagaatt aacaatgaaa caatggttac
     1981 ttttagctat tgcggactct gtagatgaaa ctcttacatt aaccaaactt ggagaattaa
     2041 tggggtgctc aagacaaaat gtaaaaaaac tagctattgc tttagaaaag aaaggtttta
     2101 tacaaattga gcaaaatgaa aaagattcaa gagctatctg tcttgtgaca aaagatagag
     2161 tgcaagagta ttctaaaaaa gtaagaggta tgcaggaaaa agtattacag cttttatttg
     2221 aagattttac agagaaagaa gtggaacagt tattttttgg aattatgaaa ttaagtatag
     2281 gaattgaaaa tgtgaaaaat tatgtagaaa ataatgacgt tggagaataa gcaagtatca
     2341 aatcaacttt gtatgacatc agagaggggg gtaatttata tgaaaagtgt agtagtgatt
     2401 tataagagta agtatggttc aacaaaaaaa tatgctaaat ggctttcaga attattgtct
     2461 tgtgatattt ttgaaactaa gaatattaat atagaaaagg ttatgaagta tgatacgatt
     2521 attttaggag gaggtcttta tgcttgtaat atagcgggaa tttctttctt aaaaaaaaat
     2581 tataaagatt tgcagaaaaa aaatttagta gtatttggcg taggagctgc tccatatgat
     2641 gatgaagtaa taaagaaatt aagagaagta aactttaagg atgaattaaa caaagttcct
     2701 tgcttttatt gtcgtggggc atgggatgag acaaaaatgt cttttataga ttatactctt
     2761 tgtagttttt ttaaaaaatc tatagctaaa aaagaacgtc aaggtcttaa accagcagaa
     2821 gcacctttat tgtctggaga agcctttgat tggtgtaata aagaaaattt aactccaata
     2881 cttaattata taagaaaact ttaagaggta t
//
LOCUS       DAMJVX010000279         2861 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.286, whole genome shotgun sequence.
ACCESSION   DAMJVX010000279 DAMJVX010000000
VERSION     DAMJVX010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.286"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            379..873
                     /locus_tag="PC407_003560"
     CDS             379..873
                     /locus_tag="PC407_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2498965.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIIALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKNDYGNLIDITNDQNKINIVIKLLNN
                     KSNT"
     gene            1270..1420
                     /locus_tag="PC407_003561"
                     /pseudo
     CDS             1270..1420
                     /locus_tag="PC407_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1896..2084)
                     /locus_tag="PC407_003562"
     CDS             complement(1896..2084)
                     /locus_tag="PC407_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2498966.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGEGALKIKKFSILYGSS"
     gene            2246..2596
                     /locus_tag="PC407_003563"
     CDS             2246..2596
                     /locus_tag="PC407_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HDF2498967.1"
                     /translation="MIQKKIFMGFCGFLGFLSLRYFSSGNVTDLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNVELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            2598..2798
                     /locus_tag="PC407_003564"
     CDS             2598..2798
                     /locus_tag="PC407_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2498968.1"
                     /translation="MPKFICNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
BASE COUNT         1055 a          332 c          363 g         1111 t
ORIGIN      
        1 tgattttata gatacacagt tagatgattt tataatagag cataaaaaaa atgtagtata
       61 atcttgttta tatcaagtta tggtattggt aagtaagtat aattaaatag acattatttt
      121 ctgggtcctc tttttatttt catctgcttt taaataatat tattgacatt gatatgactg
      181 tagttttctt tattttaatc cattgcttat atcttttcta acaagaaatg tcattttaaa
      241 tcatttctat aaattgtaaa ataatagtat atttgtcata gaaagtgatt tttttgtcta
      301 tatatggtaa aatatagaaa gtagaaatca acaaaataaa aatttatata ttattaattt
      361 tatatggagg ggaaataaat gaaaaataaa aaaggattta ctctagtgga attattagta
      421 gtaattgcta taataggaat attggcaata atagcacttc cagcattatt taaaaatata
      481 gaaaaagcaa agatagctaa acttgaagct gatataagtg caataaaaag tgcatctctt
      541 agttactatg ctgatgaatc caagtatact gatggaggaa tgatatcatg ggtaaagaaa
      601 gatggaaaaa taataataaa tgggggtttt aaagatgacc cattagcaga taaaatagaa
      661 aatttaggga tgccttataa tggttcatat ctgttaatgt catctcctgg tcatgaaaaa
      721 tatctagaat taagcatact tccagaagga gaaataagca aaagtggtct agataaatta
      781 aaaaatgatt atggaaattt aatagacata acgaacgatc aaaataaaat aaatattgta
      841 ataaaacttt taaataataa atcgaatact taaatacaat aaatttgatt taataattac
      901 tgatgaaata aacaatcttg tttggagaaa tgaaaaaagc actccccaag aatagaatgc
      961 ttagttataa ctacagaagt gtattggcat acacttctat tttttattat atcacataat
     1021 ttatgttgct aaaaatacct atttaagttg tttttaaatt gtgttgagta atattcttga
     1081 tagtttatac attaatgaat ttcaaaaaaa taagcactct ttataataaa aagtacttta
     1141 gtgtatgtta atgtcctatc taataaaatt gtaacatgtc ttatgcttta gtatgataat
     1201 tttcattctc tttattgttg caacttatac tatagttttg ctaatttatc tgcaaacaca
     1261 acttctttat tgcatgtgta tcaaccaaaa acatcagaat acttacttaa tacatctgca
     1321 aaatcgtatc tttcatctat agtattctta tttgacaata taacaaaaaa tgaatcgaga
     1381 tatgcctttt ttataattag atatatttta taaattctag catatcattt gttattttct
     1441 gatatgctat tgatgtttta aatcttctat ttattttatt attttttact tttccaattg
     1501 tacctttata ggagttatat ttatgtgatt tatgaaaaaa tgctatgcat ttaagttcta
     1561 attttttgat aatacatcta actttctttt gattaataat gataccacta tttttaatgt
     1621 ggcttccctg aggcacacat cttttttaaa aactcaagct catcttttaa aaatttattt
     1681 tcatattcta attcttgttc tcttgttaat ttattttttc tttagtgttt ttgtttttat
     1741 tcattatggt cttcctttca ttttgaaaaa ggcatcaaca ccactatgta aaaatttttg
     1801 ttttcatgtt gctataactg tgctattgtt tattccaaaa tacttagcga tttattgtac
     1861 actatactta gttatcatca tatagtttat aacatctaac ttgaaccata tagtatagaa
     1921 aactttttta tttttagtgc accctctcct aatatattaa cccatctttc aacttgagaa
     1981 tggtcataaa ctccatattg ttttactatt aaaatatctc ttccttctct attcaaataa
     2041 aatttagcta cttttaattt gaaattaaaa tcatatttag tcatacataa acatctcaag
     2101 cgttagattt ttactctaac aattatggtg cagtacaaat attgtataat actgattttc
     2161 tattgacaaa agaatataaa agagatatat ttgtattata aagatacaaa tatatctctt
     2221 ttaaaattac taaggaggat aaaaaatgat tcaaaaaaaa atttttatgg gtttttgtgg
     2281 tttccttgga tttttaagtc ttagatattt ttcatctggc aatgttactg atcttacata
     2341 tattggattt tttgcttttt tctcaaactt tattatagca aaaataaatg gtgataaagc
     2401 agatgaaagg tatgtacaag atgaaaaagc agctatggca tttacaggac agcttgctat
     2461 tatagaatta ttcattttat ggtgtattac tatagtgtct aggaatgtag aattaatgtg
     2521 tgttttacta tcaattacat atgcaatcac tttaaatgtt tatgctataa aactttatat
     2581 tttagaggaa aaataatatg cccaaattca tttgtaacct aaaaaaatat agacaattaa
     2641 aagaattaac tcaagaacaa ttagcagaaa aagtaggagt tagaagagaa accattatgc
     2701 gtttagaagc aggaaagtat aatccttctt tgaaattagc aatagatata tcaaaaatag
     2761 tagatacacc tattgatgaa ttgtttattt ttgattagtt tttgcattat taaaaatgtg
     2821 ttttaaatac aaacttaaat tcaatcttta agtcactcct g
//
LOCUS       DAMJVX010000280         2826 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.287, whole genome shotgun sequence.
ACCESSION   DAMJVX010000280 DAMJVX010000000
VERSION     DAMJVX010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.287"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            322..624
                     /locus_tag="PC407_003565"
     CDS             322..624
                     /locus_tag="PC407_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498969.1"
                     /translation="MKYSKGFKLIYSLVEYSKKFKVIYSVMKYSKGFKLIYSLVEYSK
                     RFKLIYDVMKYSKEFKLIYSLVEYSKRFKVIYSVMKYSKRFKAIYNAVKYIQGDLK"
     gene            836..1105
                     /locus_tag="PC407_003566"
     CDS             836..1105
                     /locus_tag="PC407_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2498970.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIGQCRKWLEETVSN"
     gene            complement(1322..1567)
                     /locus_tag="PC407_003567"
     CDS             complement(1322..1567)
                     /locus_tag="PC407_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HDF2498971.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            2104..2664
                     /locus_tag="PC407_003568"
     CDS             2104..2664
                     /locus_tag="PC407_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin regulator"
                     /protein_id="HDF2498972.1"
                     /translation="MFFGNISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAE
                     PWFKMKDDEKKAIEAENQRKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYK
                     YKKVKFEGKVIQVIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGI
                     SAGTITYESTMGGKITIPGIAVEKIN"
BASE COUNT         1042 a          298 c          422 g         1064 t
ORIGIN      
        1 ttaatgcaaa aacaattttt atattatata atgaaactta attaaatata gctaaaaaat
       61 cattcaatac attaaataaa tatttaactt taataatcta tatttaatta tggctataac
      121 atagaattat caaatatatt gaatgaatat ttgactcttg gaatgtgtaa ttaaattaag
      181 tttggttatt cgatggaagg caaatttgat ataaaaactc catttaaagt gccagataaa
      241 aactttatgg ttatataaaa gttaaaagta atttatagtg ttatagaata ttcaaaggga
      301 tttaaagtaa tctatggtgt tatgaaatat tcaaagggat ttaaactaat ctatagtctt
      361 gtagaatatt caaagaaatt taaagtaatc tatagtgtta tgaaatattc aaagggattt
      421 aaactaatct atagtcttgt agaatattca aagagattta aactaattta tgatgttatg
      481 aaatattcaa aggaatttaa actaatctat agtcttgtag aatattcaaa gagatttaaa
      541 gtaatctata gtgttatgaa atattcaaag agatttaaag caatctataa tgctgtgaaa
      601 tatattcaag gggatttaaa gtaatctata gtgttatgga atattcaaag ggatttgaag
      661 taatttattg tgttttgaaa tattcaaaaa tatttttgga atagttttta taaatttacc
      721 agtattaata attttatgtt taacatttag atgacttaaa atcaactatt tgcacttttg
      781 tagtcatcta aaatcatttt ataatatttt taaataatgg aaaggatgtt tgaacatgtt
      841 tgaatcaaga tgtggtgtct gttgtgacag ttgtacaaaa aaagaacaag taaattgcac
      901 tggttgccca actatggaaa aaccattttg gggtggagaa tgtaaggtta aaacttgctg
      961 tgaaagtaag gaacttaatc attgtggtga atgtgatact ttcccttgtg atatgctttt
     1021 aaataaggga aaagaccaag gctttgaccc tatggtaaat attgggcaat gtagaaaatg
     1081 gcttgaagaa acagtttcta attaattgtt ttagaatgct ttgttaagta atttatttaa
     1141 tcctttgtta ggtagtatgt ttagtcattt attgattggt atatttatac atttattgat
     1201 gggtatattt agccatacaa atagatttac ataaaattta cgactaaaaa agagttgagt
     1261 attagttttt attatttaaa cggatattca actctttttt atatctaact tttttgattt
     1321 tttaatcttt ccaataccac cactttaatt tttctggttc tggattttca gttgatgcaa
     1381 aatatactgc acatctgtat acatataact gacatctgtc tatgtgctgt ccacgcatta
     1441 aacattcctt ttcatacatt tgtgctgggt ttgcatcttt aagtgattta atagttgtat
     1501 atcctaacat gattaaagat ttttctgttg ctttccctac ccctggaatt ttttgtagtt
     1561 ctcccatact tcaccttctt tctgatatat tatttttgta ttatacttag taccagatat
     1621 ttttttatta tagttaatat ttaatttttt attatatcac tttatttatg ctctttcatc
     1681 tatctatatt ttaccacctc taaagtactg aatcatttaa ttacatcata atatagtttt
     1741 atacaaataa aatactttat gtttcattta atatataaaa ttcaccttca agaaaattat
     1801 attataatct gacattttta cctcattttt caaaatatat tgaatcttct tgatttattt
     1861 tgtaaaatta tgcttagggg aaatatattt taggaaaata tgaatatata atttttagtc
     1921 aactagttat tttaagtttt taaattttaa aataaaatat atctaataaa agggagattg
     1981 tattatgttt tctaaaaaaa atgagggtaa cgaatttagt aatgaaagaa aaggaagctc
     2041 taagaaaata attaaattct ttaagagcac aaaggatatt gctctactgg catttatttt
     2101 ggtgtgtttt ttggcaatat atcctcacca gcttgttctg aagaccatga ggaggtcatt
     2161 tctaatcaaa catcagttat agattctcaa aaaacagaaa tagaaacttt aaatagcaaa
     2221 ttgtctgatg ctgaaccatg gttcaaaatg aaagacgacg aaaagaaagc tattgaagct
     2281 gaaaatcaac gtaaagctga agaagctaaa aaggctgaag aacaacgtaa aaaagaagaa
     2341 gaagagaaga aaggatatga tactggtatt acttatgacc aattagctag aacacctgat
     2401 gattataagt acaaaaaggt aaaatttgaa ggtaaggtta ttcaagttat tgaagatggt
     2461 gatgaggtgc aaataagatt agctgtgtct ggaaattatg ataaggtcgt actatgtagt
     2521 tataaaaaat caataactcc ttcaagagtg ttagaggatg attacataac tataagaggt
     2581 ataagtgctg gaactataac ttatgaatca actatgggtg gaaaaataac cataccaggg
     2641 atagctgtag agaaaattaa ttaatattgt tttgtattat agttaatatt ttatattata
     2701 gtcaatatgt ttaaagatgt ttttataatt gcaaataaac agttacaagg ctctaaatta
     2761 gtttttgctt ttagcatatt atctattttc tatcaactat taattattta gtattaatat
     2821 ttccat
//
LOCUS       DAMJVX010000281         2824 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.288, whole genome shotgun sequence.
ACCESSION   DAMJVX010000281 DAMJVX010000000
VERSION     DAMJVX010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.288"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            215..1036
                     /locus_tag="PC407_003569"
     CDS             215..1036
                     /locus_tag="PC407_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2498973.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    1115..1326
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1413..2729
                     /gene="argH"
                     /locus_tag="PC407_003570"
     CDS             1413..2729
                     /gene="argH"
                     /locus_tag="PC407_003570"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDF2498974.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
BASE COUNT         1154 a          253 c          551 g          866 t
ORIGIN      
        1 aatttctagc attataagac tataaattga aaatattcag aaaaatacta caagaaacga
       61 tttattttgc tcttaaaata agttaaatga atgttattaa ttttgtaaat tgaaatagtt
      121 taagtataaa gttatgatat aatctaataa gggtaattct ttagattata ttaaggaaat
      181 ataggtatac aataaaaagt tatggagttg gattatggga tataaattaa ttgtaacaga
      241 tatggatgga actttattag gcaataatca taaagttaca gatgaaaata aaactgcact
      301 acaaaaagtt ataaaaagcg gtattaatgt aactttagca acaggaagag ccttcgattc
      361 agctaaatgc aatgtagatt ttcttaaaga ggacatgcca ataatagctt gtaatggttc
      421 attaataaga gagcaaaatg gaaatataat atattcaaat aagattgata caagaacatg
      481 tttaaacatt ttagatgtat tagataaata cgatatttac taccaatgta atagtataga
      541 ttctatgctt tcgaaaaaaa tagaaggacg tgaagataga ttaagtgtat ttttaggctc
      601 tgaaacagaa gtaattgtaa aagatgattt gagagaagaa atctttaaaa aagatatact
      661 aaaatttgta atcatagaag aaaaaaatcc atctattttg gatgaaataa gaaaagaatt
      721 gagaaaagtt caaggaataa agataacttc ttcatggcca aacaatattg aagtcatgaa
      781 tgaaggtgtt gataaaggta atgcagttaa aattttagct gaaaaaatga atatagacag
      841 agaagatata attgcatttg gagacaatta caatgacata gaaatgataa agtttgctgg
      901 tcttggggta gctatgggaa atgcagaaga attgattaaa caagaagctg attatgttac
      961 agatacaaat caagacagtg gagttgcaaa ggcaatttat aaattcctag agttaaaaga
     1021 atcacttagc tcttaaaaca gtatataata ggaaaaaata ggttgactat tttgtagata
     1081 gtaattataa tacaaataga taaaacataa aaatttagat tcagtgaaaa agagagtaga
     1141 tatagagaaa atctcagcga attgggggat ggtgtgagcc taatgataac actttatatt
     1201 gaaaagaact ttggagaata ctacttgaaa ttcagtatgg tagtaggtaa tctcgcctta
     1261 agagaataga gtgggtaaac attgtttacc aactagagtg gtaacacgga atatttcgtc
     1321 tcttggtctt tttgaccaag agatttttta gtttcattga gttacaagac ttataattaa
     1381 aatacaaaaa attaaaatat gaggtgaaag ttatgaaatt atggggcgga cgctttagaa
     1441 aagcggaaaa tcaacttatg gaagaattta ataaatcttt tggctatgat tgtgtactgt
     1501 acaaaaaaga tatcgaagga agtgtagcac atgttcatat gcaagtaaaa tgtggacttt
     1561 taacagaaga agaaggaaag tctataacag aaggtttaaa agggatactt gaagatgtag
     1621 aaaatggaaa gttagtattg gatggagagt atgaagatat acatagtttt actgagataa
     1681 atttaattca aagaattgga gatgttggca agaagcttca tacagcacgt agtagaaatg
     1741 accaagttgc agttgatatg agattgtatg ctaaagaaaa agcaaatgat ttggtgggtt
     1801 taatatctga atttaaagct actataaaag atgttgctga caaaaaccca gtgatgatgc
     1861 caggctatac tcatcttcaa agagctcaag tagtgacatt taagcatcac ttaatggcat
     1921 attatagtat gtttgataga gatgagaaaa gaataaaaaa tgctatagaa atattagatg
     1981 aatcaccttt aggctgtgga gctttagcag gaactacgca tgatatagat agaagtataa
     2041 cttgtgaaca attaggattt aaaaaagtag tagataattt tatggatgga gtaagtgata
     2101 gagattattt actagagcta atgagtgatt tttcaattat aatgatgcac ttaagtagat
     2161 taagtgaaga gttgatactt tggagttctc aagaatttgg atttgttgag atagatgatt
     2221 tatatacaac tggaagtagt ataatgcctc agaagaaaaa tccagatggt gcagagctta
     2281 taagaggtaa gactggcaga gtatatggaa acttatttgg tctttttaca gtaatgaaag
     2341 gaataccatt agcatataat aaagacatgc aagaagataa agaaggtttt ttcgatagtg
     2401 tacatactct tgaaatgtgt atacaaataa tggatagaat gatagccaca ctaaaggtaa
     2461 atgaggataa aatgaaacaa gcagtaaaaa atggtttttt aaatgcaaca gaagttgcgg
     2521 attatttggt taaaaataat gttgctttta gagatgcaca tggtatagta ggaagtatcg
     2581 ttatatattg tgaagataat aaaaaagcaa tagaagattt aacattagaa gaattacata
     2641 agttttcaga tgcttttaag gaagatattt atgattttat cgactatgag agtatattaa
     2701 ataagggaat aaaaaagaat ttaaaataat gtatttattt ttaaaagctg taaatttgaa
     2761 tgttatcatt taaatttaca gctttttatt gtgtaagatt ttattatata aaagtaagaa
     2821 actt
//
LOCUS       DAMJVX010000282         2806 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.289, whole genome shotgun sequence.
ACCESSION   DAMJVX010000282 DAMJVX010000000
VERSION     DAMJVX010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.289"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            408..1451
                     /locus_tag="PC407_003571"
     CDS             408..1451
                     /locus_tag="PC407_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498975.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKG
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            1562..2704
                     /locus_tag="PC407_003572"
     CDS             1562..2704
                     /locus_tag="PC407_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2498976.1"
                     /translation="MSEEKNPKENTLDEPKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         1266 a          225 c          493 g          822 t
ORIGIN      
        1 tacatataag atataaggtg atagatagaa attgttggat tatgtaaata attaatatat
       61 aataccatta tgggcaataa atacaaatca aatgataata tagtctattt atgtaaatgt
      121 tatattgtat cctaagtata atagtaagtt ttttgatagt ggtgtagaaa gtaaaaatct
      181 actagcctaa attgatgtat ggattagttt aggaacagta attgcatatc ttaagtttga
      241 tgtcatgttg ggctactaga aataggtcat ctttttcact tgaatatctt ttctttaaac
      301 atgaggagta tcatatcaaa aacatatgaa aattaagtga tttataagaa cagtaatatt
      361 ttatatgata gtatagaata gtaataaagt attaggagga acattgagtg ttaaaaaagt
      421 ggtttggtat tgtgaaaaaa agacaaaaaa gtgagtcagt aaaagaagaa ggtgaagtaa
      481 tattaaaaaa cgaaaaaata ttatctgaag aaaagttgat agatgaagaa ggagttgtag
      541 ttaatattga taatgaaata ttaactaaag tagaagtagt aaatgatgat aatgaaatag
      601 aagaaaaaat aatagaagaa gattggttaa ttagtgaaaa taccataaaa ttagatgata
      661 aagaagcaat tattaatgat aaaaacatag aattatgtaa aaaagaagtt caagttgaaa
      721 gtgaaaaaat aggtttaaat aagtttgaag gacttgacca aaatgaaaat tataatctag
      781 aaaaaaatgt tattgaagaa aaaaaagtaa gtgaatgttt gacagaagaa gatttagagt
      841 acataaaaga aattaaaata aaaagaggaa aaagtataaa agctataaac ttgtatacta
      901 aagaagagtg ggtttttgac actcatatac agtgtagtaa aaaactcaaa gttccattag
      961 gatacataag agaaaattta aaatatggat atatggatta ctttggagat gcaataaatt
     1021 atttaagtga agtattaaat atagatgaat actgcaaagg tgaatggagc tatctcgata
     1081 atagcaaatc tccatctgag atatttaata ttctaaacaa taaaatattt agtataaggc
     1141 tttctaatga aaaaagaaat gaaattttga caaatgataa aattgaagca ttaaaaatga
     1201 attatagatt tgaatgtatt gatgaagaat atgatgaata ttttaaaaaa tataagtcta
     1261 taatcaaaag gggtggaaag aaaaaagttg aattagtaaa taaaaaaggt gacattttag
     1321 aaatatttaa gtctttagaa gaatgtgcca tttatttgca aaaggaaaag aatgaagtta
     1381 tacaaatgtt aaaatatgga gatacaaaag taggaagaaa ctttataagg tatagtttga
     1441 gaagtattta atttcatttt aaattcataa gagtttcatt tctgattcac acatttaata
     1501 ttattttaat gtaaaataat aaaggtgaag tattgcacaa ttaaaattaa ggagggtatt
     1561 aatgtctgaa gaaaaaaatc caaaagaaaa tacattagat gaacctaaaa attcagataa
     1621 tgtaaattct aaaaaagatg atgtgtcaga tgaaaaaaat actgataaca aagttaaaaa
     1681 aagggaaata aagctaaaca acaaaacact taaagctatt gtagcaattg ttgcagtagg
     1741 agctatatgt ttaggtggag gctatgtttt tggtaaagaa gctggaagaa aattgcctgc
     1801 tacttcaaaa cactataata gcagtgaggt agtagctact attggggata aaaaaataac
     1861 aggtgaaatg ttaagaaaga gaatggagcc attattttat ttaaatgcaa aaactactat
     1921 gactgatgac gaaatacgtt catatgaatc aaatatgatt gaaactatga ttacatctga
     1981 tatattatcc tctgaagcag caaaagagaa aataagtata actaatgacc aactagatgc
     2041 tcagtataat aatttaatga gtagtataga atcagcaatg ggaatgacta aagaagagtt
     2101 tttaaaacag tttgatttga ctgaaaaata tataaaagat gatatgaaaa aagagctagt
     2161 agcatcaaaa tatttaaatg aaaaatcaaa agttactgat aaagaagcag agaactatta
     2221 caataaaaac aagagcaatt atttacaagt tagagcttca catattttaa taaaaactgt
     2281 tgatgataaa ggtaagcaag ttagtagtag caaaaaagct gaacttaaga aagaagctga
     2341 agaaatactt aaaaaagccc aagctgggga agattttgct actctagcta aaaaatattc
     2401 tgaagatagt agtgcagaat caggtggaga tttaggtttc tttggtaaag gtcagatggt
     2461 ggaatctttt gaaaaagcag cctttgcatt gaagaaggga gaggtatcta acaaacttgt
     2521 agaaagtgat tatggatatc atataattaa gaaaactgat gaaaaatatc aaccatttga
     2581 agaggtaaaa tcagatttag taagcagttt aacatcagaa aaacaaaacc tacttataca
     2641 aaatttaaaa gaaaaatata atgttaaaat tacagataaa tatcaaatga aagctaaaga
     2701 atagataaag tcaatttatt aaaaaaaaga aaaattaact gtatggataa aagctatttt
     2761 tactctcaat atagagagtc taaagtagct tttattattt tgtaat
//
LOCUS       DAMJVX010000283         2771 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.290, whole genome shotgun sequence.
ACCESSION   DAMJVX010000283 DAMJVX010000000
VERSION     DAMJVX010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.290"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            185..1108
                     /locus_tag="PC407_003573"
     CDS             185..1108
                     /locus_tag="PC407_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HDF2498977.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESINLTKTKIY
                     SNLRDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDGLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLNKKDEDIYKILSLNLLKIIALGNDNSLKGTKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1083..1910
                     /gene="panB"
                     /locus_tag="PC407_003574"
     CDS             1083..1910
                     /gene="panB"
                     /locus_tag="PC407_003574"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDF2498978.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGAKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIADMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGVFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1929..>2771
                     /locus_tag="PC407_003575"
     CDS             1929..>2771
                     /locus_tag="PC407_003575"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDF2498979.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNINTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PKNIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELN"
BASE COUNT         1097 a          337 c          459 g          878 t
ORIGIN      
        1 tatagaatta attgtcaatt ttttatgaaa atggtaagat atttatggat ggtatcttgt
       61 tataagaact atactttaaa tgtttttaat tattttgtct tatgtaactc atatcctcag
      121 gaatgagatt agaaattaag atgttgtatc acaaagtgga ggtgatacta ttaaaatagg
      181 ctttattggt tctggaaaag ttggaacatc tttggcaaat tattttttat caaaaggtta
      241 ttgtgtttca ggcttttatg gaaggaatca attatcttta ttagaatcaa taaatttaac
      301 aaaaacaaag atttacagca atttgagaga tataatttat gaaaacgata tattatttat
      361 tacaacatca gatgattcta ttgaaattat agacaaaaaa ctttccaaat ttaatttaaa
      421 acataaatac atttgccaca caagtggctc tttaaaatct agtatattgt tctgttcaaa
      481 aaaagcaggt gcattaattt attctatcca tcctatattt gcattttcca ataaaaatac
      541 caacataaaa aaaatgaaag acatttgttt ttccatagaa ggtgataatg aacaagatga
      601 tttagttatt caaaatttca tagatggttt gggaaaccaa ttttttataa gagataagga
      661 tacttcatcc acttatcatc tagctaatgt tttagtttca aatttagtat tatctttact
      721 agatatagga gtaagttact ttattaaatt aggtttatct gaagaggaat ctttaaaagc
      781 tatcagtcct ttagtaaaaa aaaatataga aaatatactt gataatggct ttactaaagc
      841 tcttactggt cctgtttctc gtggagatat aacaccaata aggaaacatc tatctgtatt
      901 gaataaaaaa gatgaggata tctacaaaat actatcctta aacctactaa aaataattgc
      961 cttaggaaat gacaattcat taaaaggtac aaaaaatatt actatagaaa atgcaatcga
     1021 aaatttaata agtaaatctg aaaaatacaa agaaatttac aaacttttag gaggaaaaaa
     1081 cgatgaaaaa taccatacag acttttaaga acgctaaata tgaaggaaaa aaactatcta
     1141 tgcttactgc ttatgactac tcaattgcaa aaataatgga tgaatgtgat ataaatggta
     1201 ttttaatagg cgattcatta ggaatggtca taaaaggtga agaaaacact ctatctgtta
     1261 ctatagacga aattatatat catacaaaag ctgtaaaaaa tggtgcaaaa aatgccttaa
     1321 tcgtaagtga tatgcctttt ctatcttacc atgtttctat agaagatgca gttaaaaacg
     1381 ctggtagact tataaaagaa ggaggagctc atgctgtaaa gttagaaggt ggctcaaacg
     1441 ttataaagca aatagaaagc attgtaaatg ctcagatacc tgtaatgggg catttaggat
     1501 taactcctca atcagtaaat tcatttggtg ggtttaaggt tcaaggaaat accagtgaaa
     1561 cagcaagaca acttattgaa gatgctaaat taatagaaaa agcaggtgca ttctctattg
     1621 tccttgaagg agttcctacg aaaatagctg atatggtaac taattctatt tcaattccta
     1681 caataggtat aggggcagga attaactgcg atggacaaat tttagtttat caagatatgt
     1741 tgggagtgtt tggagatttt gttcctaagt ttgtaaagca gtatgcaaat ataggagata
     1801 taatgaaaga ttcaattaaa aattacatcc tagaagttaa tacaggtgca tttccacaag
     1861 aaaaacacag cttttcaata aatgaatctg agttagaaaa attatactaa tattaaggat
     1921 ggtacaaaat gcttgtaaaa gaaattaaat tactaagaaa tataatcaaa gattggagaa
     1981 aacatggcta ttctataggg cttgttacaa caatgggatt tttacatgaa ggacaccaaa
     2041 gtcttataaa aaaagctgta aaagaaaatg ataaagtagt agtgagtgta tttgtaaatc
     2101 caactcaatt tggaccaaat gaagatttta atagctatcc aagagatata gataaagatt
     2161 ttaaatattg tatggatagt ggagcaacag tagtctttaa cccatcacca gaagaaatgt
     2221 atttaaaagg taattgtact accattaatg tttctggact tacagatttt ctttgtggag
     2281 ctaaaagacc tgttcatttt ggaggtgtct gcctagttgt ttcaaagttt ctaaatatag
     2341 taacacctga taaagcttat tttggagaga aggatgccca acaattggct gtaattaaaa
     2401 gaatggtcaa ggatttaaac ataaatacag aaataatagg ctgtcctata attcgtgaaa
     2461 atgatggatt agcaaaaagt tcgagaaata catatctctc tgaggaagaa cgaaaatctg
     2521 ctctaatatt aaataagagc ttaagtttag caaaagaaga attagtaaaa ggcaatctaa
     2581 atccaaagaa tataaaagaa ttaataacag ctaaaataaa ttctgaacac ttagcaaaaa
     2641 tagactatgt tgaaattgta gatagtgaga cattacaacc tgttaagcaa atagaacatt
     2701 ctatccttgt ggcaattgct gtgtttatag gaaaaaccag actaattgat aattttacat
     2761 ttgagttaaa t
//
LOCUS       DAMJVX010000284         2676 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.291, whole genome shotgun sequence.
ACCESSION   DAMJVX010000284 DAMJVX010000000
VERSION     DAMJVX010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.291"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            338..2149
                     /locus_tag="PC407_003576"
     CDS             338..2149
                     /locus_tag="PC407_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2498980.1"
                     /translation="MKKKYDFLIYSAIWLVIIIFFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILYNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2237..>2676)
                     /locus_tag="PC407_003577"
     CDS             complement(2237..>2676)
                     /locus_tag="PC407_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498981.1"
                     /translation="MEFEDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHYWD
                     EKVATEKTFINGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGS
                     EDGEYVELTNDSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         1009 a          279 c          396 g          992 t
ORIGIN      
        1 aaatatagat tatgtatatg ccaaatttaa aaagagacat aaagacatta tacaaatata
       61 ttattggggt gatattttat aagaaaagaa gtgatgcagt aaattaattg agcaatttaa
      121 aaatgattta atgatagtgt tgatgaatgt ttaaaaagaa attttagaaa aacgtatatt
      181 gctgtttgtg ttggaggtga tgaatttgta taatttatag ggtataaatg attaaactac
      241 ttaagataaa tacatatcac tgtaaaatat tgataatcat tttttataag agattgatta
      301 tttgcaaatt atatatatta caaatgaggt aacaataatg aagaaaaaat atgatttttt
      361 aatttattct gctatttggc tagttattat tatttttttt atattttctc tatatcatag
      421 tatagagcat attaaaatta taagtcacac tggtactatt cgcagtgaaa ctcaaaaggt
      481 tgttaagcaa gaattgaata atgaaagaaa tgatgacttg ataaaacgac tggataatat
      541 tctgataaag ctaagaacag gtaatggaga aaatggattt caaagatgtg ataataaaga
      601 atttcaacaa aaactaaatc aaatggattc tatgtgggaa tctatgaaga aagaaattat
      661 aaaagttaga aatggtgctt caggtgataa attatataaa ttgagtgaag agtattctgt
      721 gttgtctaat caaattgttt ttatatctga aaaacactct aatgcaaaat tgtattcttt
      781 tgctactgca ctatttattt atcttatatt ctcaacaata agtcttttaa tttgggaata
      841 ttataataaa aaaaggttta aaagaatatt ttatactgat aatttaacaa aaataaaaaa
      901 tcaggttgca tttgaaaaca gagcgattga aattttgtac aatgcatcta acaaagagta
      961 tgtactgcta aatattgata ttgacaattt taaatatatt aatgatacac atggatatga
     1021 atatggagat aaagttctaa taatagttgc agctgcactt tctaaaactt ttaatatcaa
     1081 agaaacttgt gcaagaattg gttcagataa ttttgtaata cttgcaaaat atagagattc
     1141 tcttttagag gatattcggg aaatgctaac taatgctata atctctgaat tagatatgaa
     1201 tgtaactcaa actatatctt attgtatagg agcgtactta gttgaaattg ataatttagg
     1261 ttataaatcc atcaattcta taatggataa ggcaaatatt gcacacaagg taagtaaaac
     1321 aagaggaatt tcctccacag tatggtataa tgaaaatctg ttaaaacagt tacaaatgga
     1381 aaatagtatt tataattata tgtataaagc gatagaaaat gaagaatttc atatgtattt
     1441 gcaacctaaa tttcaaatat cttctttaaa tgttgtaagc gcagaagctc ttgtaagatg
     1501 gttttctcca gaacttggtt ttttatcacc agatgaattt attccattgt ttgaaaaaag
     1561 tggatttatt attgaattgg acttttatat gttaaaaaaa gcgtgttcat ttgtaaaaaa
     1621 aacgtttatg aaaaaaaatc aatatacata tccgattgca gttaattttt caagggttac
     1681 tatttaccaa aatagctttt atcaaaggtt tttagatact gttaaagaat atgaaattcc
     1741 ttttaagtat atagagatag aggtaactga aagtgctttt aatgaaatat ctcaaccagt
     1801 tatttcgatt ttagaggagc ttaaaaagtt aggattttta atttcgatgg atgattttgg
     1861 atcaggatac tcttcattaa gtcttttatg ttctctatct attaatggat tgaagttaga
     1921 taaaagctta cttaaagaaa catttaaccg ggagaaagta tatagtatta ttcagtgtat
     1981 tattgaaatg tcacatcgta taggcatgtc tgttgtatgt gaaggtattg aaactaaaaa
     2041 agacttggaa tttttaaata cagttaaatg tgatgtagga caagggtttt atttctctaa
     2101 accgatagaa gaaaaagaat tttttaacaa atatgtaact aaaaaataat ataattattt
     2161 ttatcagtct atacattgag tcattttata tttcgttaaa tgactcaatg tattgttatg
     2221 tattttaaaa tataaattat ttaaaacgtt ttttgtactt atatagaaat tcttctatat
     2281 tttttgattg ttctcttatt tcaactgctt ctatatctag taaataaagt atagaatcat
     2341 ttgtaagttc aacatattca ccatcttctg aaccgagtgc atgtctctca atttttaatc
     2401 ctttttcttc ataatatttt tttgtttcta tatataatga ttctcgcttt tgtatgtagt
     2461 ctttcacttc attatttgga aagttatatg aatccactaa tgtttcgcca tttataaaag
     2521 ttttctcggt agcaactttt tcatcccaat aatggatttc ttcccaagta aaaaatatct
     2581 cagttccatc tggaatgaat gaaaacctgg taataatata taaataatta ttgaaattta
     2641 cttcataata ttcaggaata tcttcaaatt ccatat
//
LOCUS       DAMJVX010000285         2673 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.292, whole genome shotgun sequence.
ACCESSION   DAMJVX010000285 DAMJVX010000000
VERSION     DAMJVX010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.292"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            1000..1389
                     /locus_tag="PC407_003578"
     CDS             1000..1389
                     /locus_tag="PC407_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2498982.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            2134..2604
                     /locus_tag="PC407_003579"
     CDS             2134..2604
                     /locus_tag="PC407_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF2498983.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIEFVKQCENSKE"
BASE COUNT         1205 a          167 c          472 g          829 t
ORIGIN      
        1 ataattaatg aaaaaattta aattttgtaa tatataaata aaaaaactct aaaatagaag
       61 gatataatta tatagaatgt ttcaattaaa caaggcaaaa actataatct taaaattaaa
      121 attaacatta ttcttaagaa gtaagaaatt taagaagtaa aaagtttagt gataagtggt
      181 agtaagcaag aaatttagaa agtaggaagt ttagtgataa gtggtagtaa gcgagaaatt
      241 tagaaagtgg gaagtttagt aataatttgt agcaagtaag aaatttagga agtaggaagt
      301 ttagtaataa tttgtagcaa gtaagaaatt tagggagtag gaagtttagt agtaataatt
      361 tgtagcaagt aagaaattta gggagtagga agtttagtaa taatttgtag taagtaagaa
      421 atttagaaag taggaaattt agtaataatt tatagtaagt aagaaattta gaaagtagga
      481 agtttagtaa taatttatag caagtaagaa atttagaaag taggaagttt agtagtaatt
      541 tatagtaagt aagaaattta gtaagtgaga agtttagtta taatttatag taagtaagaa
      601 atttagtaag tgagaagttc agtaataagt tgtagtaaat gaaaagttta ggaagtagga
      661 agttcagtaa taagttgtag taaataaaaa atttaggaag taggatttac tgaaatacaa
      721 atatttaaaa tcaaagagtt taaaagaaag gagagctact ggacaagtat ccaaaataga
      781 tagctgagta caaaatatct cataaaatta agcaatataa tacaagagaa ttttgacatt
      841 atttatatat atttaccgtg tacaaatagc taaatttagg attaagattg aaaaatatct
      901 tataagtctg agcttatata aatccgtata agatgtattt aatcgaaaaa ttttattatg
      961 gatattgctt gtctatattc tgagtagcaa ggttttatca tgactataaa aaagttgcca
     1021 caatcagaat taaagataat gaaatttata tggaaatcag attccaaagt aacatctaga
     1081 gatattgttt taggaatgga gcaaaaatat caatggaaac aaacgacaac tttgactctt
     1141 ttatctagat tagtagtaaa aagatttcta aattctcaaa agatagataa atatacacat
     1201 tatgaagttt taattaaaga aaaggaatac ataggagttg agacaagaga tttttttaga
     1261 aatattcatg attcttctat aaaaagttta ttgttgtcat tacatgaaaa tattaatttg
     1321 agcaaagatg atatcttact tattgaagag tggataaaaa atctaaaaga agaggagaaa
     1381 gacgtataag caaacgtatt tcttatagca ttaatataaa gtgtatgtaa gtgtatttat
     1441 gtaatatatt agtctactta agtaatatga caaatgatta taagtggatt atgatactat
     1501 aatgtgaatc taaataaatt aaatataaat tgatagtgga tattctattt aattaaattt
     1561 aaaaagctac ctttacttat aagtataatt aaggtagctt tttaaataga ggtagtgtga
     1621 ttaaagagaa tttttaaata taaattgtga agctttaata tagatgacaa tatatatttt
     1681 gcaaatatga cacagtgttt tcgtatttaa gatgataaaa taaataggaa ttaatgaaga
     1741 tatttaaaaa ataaaaataa agaattaata gaagatatta aagagataga gataaaagtg
     1801 ttaatagaag atattgaaaa agtaaaaata aaggattaat agaagatact gaagaggtag
     1861 aaacaaaagt gttaatagaa gatattgaaa aaataaaaac aaaagattaa taaaaggtac
     1921 tgaagagata gagataaaag tgttaataga agatattgaa aaataaaaat aaaagattga
     1981 tataatatac tgaagaggta gaaacaaaag tgttaataga agatactaaa aaagtaaaaa
     2041 taaaggacta ataaaaggta ctgaagagat agagaaaaaa atgttaatag aagatactga
     2101 aaaaatagaa ataaaaaatt aggaggaaaa tttatgaaat atcaggtttc attattagca
     2161 gtgaaggata ttgaagtatc aaaaaaattt tacaaagaat tatttgagca agaaattgaa
     2221 ttggacttag gcaaaaatgt aacatttaaa ggaggatttg ctatacaaga agacttcgca
     2281 tggcttacag ggattaatcc agatacaatt atacaaaaat caaataatat ggaactttat
     2341 tttgaggttg atgattttga cgaatttatt gaaaaactca attattataa agatgtagag
     2401 tttgtccata agccactaaa acatgaatgg caacaaagag tagtgcgtat ttatgaccca
     2461 gataagcata ttattgagat aggagagtct atggaagtta tagctagaag atatttatct
     2521 gagggatact ctgtagaaga aacttctaaa ataattcaac atccaattga atttgtaaag
     2581 cagtgtgaaa atagtaagga atgattgttt aaattataat tatttagtag aaattaattg
     2641 tagtggaact aattcaagat aatatatagg act
//
LOCUS       DAMJVX010000286         2665 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.293, whole genome shotgun sequence.
ACCESSION   DAMJVX010000286 DAMJVX010000000
VERSION     DAMJVX010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.293"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(135..1520)
                     /locus_tag="PC407_003580"
     CDS             complement(135..1520)
                     /locus_tag="PC407_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2498984.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(1628..>2665)
                     /locus_tag="PC407_003581"
     CDS             complement(1628..>2665)
                     /locus_tag="PC407_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2498985.1"
                     /translation="YKHNLDNYIPYDQGITLDIFMYIWILGIIAISFYYLRTNFKFYN
                     YIENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTRCKIIIPEKK
                     YNNKELRFIFKHELIHYKRKDNLFKLLISIICIIHWFNPILYLLKKYFHELCELSCDE
                     IVIDKCTTSEIKEYAYIILNTIKYQKTLKYSSFVSQFLNKKNIILKKRLNSMFNQDKK
                     KINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIAKINYNKNGETYGTHIIDSNGNS
                     IEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFKKTIPLYDKE
                     GIKIIGRFEIN"
BASE COUNT          856 a          386 c          329 g         1094 t
ORIGIN      
        1 ttattaaatt ttatctattt gttattatga tgtaataata catattaatc tctataagtt
       61 aattactatc tggtgtaatt ttattttaac aacaaaaaaa aatacacaca attaaaatat
      121 tgtgtgcaat acaattattt tttctcttct aatagtcttt ttaatacgct cagaccagct
      181 ttaagttctt ctgtgctttc tgtagcacat acagctattc ttattgcaga aattggtttt
      241 tcttttccaa cagcaaaacg ttcagaagca tagacctgta caccttcttt aagtgcttgt
      301 atctcaaatt tttctgccat tattccatca ggtagaatca accatctaaa tatacattct
      361 tcatttccca aaagattata tccagataaa tattgattaa taatcttgtt tcttcttctt
      421 gcagatttac ggcgtgcatt aattaattta tcggcttcac ctgatgtaat catgcgataa
      481 gcaatttctg ttaaaaaata agattgagat agattaatgt tgtataatgc gcttgataat
      541 gcctctcgat actgtttagg tgtagagata tatgctagcc gtaaactagg tgcaataatt
      601 tttgataaac tcgtaatata aattgtttga ttgggcagat aggaagccac tggatttaaa
      661 tgtgtttcat taagtaaact atgaatagca tcctcaataa caattaaatt gtatttagat
      721 gcaatatttg caatcatttt tcgtccattt tgtgacatta tatgtgtagt tggattttga
      781 taatctggta ttatgtatag acctttaata ttttcatttt tacaagcata taatagtcct
      841 tcttcactaa tctcattatc ttcttgttta attggaatta gttgaatccc aagcatttta
      901 gctgctgtct ttattccagg ataagttaat gggtctacac ctattctatc tccatgttga
      961 aacaaaccag ctaaaatagc aacaattgca ttttgtccac cacttgcagg aagtaagcta
     1021 tctgatgtag tttcaagccc agctttataa attaatttgg caatagcttc tttttgccat
     1081 ttagcaccat ctctttgtcc atattggaat atagttttaa aatctatttc cttaattata
     1141 tgtttaaaca aagtattcat ttcttctagt gtgaaatctg gattcattgt gcccatttca
     1201 ataaatgtaa ttttattatt actggacatc aagaatagat ttgaacgcgt atcataagat
     1261 acaaaagttc cacttcctgt aactccactt attagacctt ttttttcaca aattttaaaa
     1321 gctctagaga tagtactcac attaacatct aagaaatctg ccaattcacg ttggggtggt
     1381 aatttagtac ctggtaataa aaatccattg ttgatatctt gttctagttg ttctgcaagc
     1441 gccttatata atatttgccc ttttgaaggt ctctttggtt tccagttcat aggataattt
     1501 tcaaaagaat taattggcat aagttttcac ctcacattat taatttgatt ttttctttat
     1561 ctaaaacaat aaattctaaa gatacaaata aaaactgata ggaaataatt ctatcagttt
     1621 ttgtctttta attgatttca aatcttccta taatttttat accttcttta tcataaagag
     1681 ggattgtttt tttaaatata ttaaaatttc tttttcttgt atataaaaca gcttcttctg
     1741 gtgtctttgg ttgattatca caatcgtaca aatctctttt ttttacgtag ccgacttttt
     1801 tacctctacc tattgtttgt ataagttctg gttcaatgga atttccattt gagtctatta
     1861 tatgtgtacc ataggtttct ccatttttat tataattaat cttagcaata gtatattcta
     1921 catcttcatt ttgtttaaaa ttggagttga tagaaaatat tgaccctatt attataaagg
     1981 ttaataaaaa cttaagacct aaattaattt tctttttatc ttgattaaac atagaattta
     2041 acctcttttt taaaataata ttttttttat ttagaaactg agatacgaat gatgaatact
     2101 tcaaagtttt ttgatacttt attgtattta atattatata tgcatactct ttaatttcac
     2161 tcgtggtaca tttgtcaatc acaatttcat cacaagataa ttcacataat tcatgaaaat
     2221 atttttttaa taagtataaa attggattga accaatgtat gatacatatt atactaatta
     2281 gaagcttaaa taagttatct tttcttttat aatgaattaa ttcatgttta aagataaatc
     2341 ttaactcttt attattgtac tttttttctg gaattattat tttacagcgt gttaaaccta
     2401 taatggcagg ggaataaata cctttaacct tatatatctg aagtttttta tttatactta
     2461 gttcttttgc ttgttcatct aagatactag taatatctat ttctaaaatt tcttttttaa
     2521 aattttctat gtaattataa aatttaaaat ttgttcttaa atagtaaaaa gagattgcta
     2581 taattcctaa tatccagatg tacataaata tatctaaagt aatgccctgg tcatatggta
     2641 tataattatc gagattatgt ttata
//
LOCUS       DAMJVX010000287         2598 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.294, whole genome shotgun sequence.
ACCESSION   DAMJVX010000287 DAMJVX010000000
VERSION     DAMJVX010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.294"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(868..1905)
                     /locus_tag="PC407_003582"
     CDS             complement(868..1905)
                     /locus_tag="PC407_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2498986.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKKTDSKKQ
                     KNIGISQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISTSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            complement(2002..>2598)
                     /locus_tag="PC407_003583"
     CDS             complement(2002..>2598)
                     /locus_tag="PC407_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2498987.1"
                     /translation="GLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLKKCLRHKTNNLEHLLEGISLDLKKYLDIKVQYLSGGQRQS
                     LSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQ
                     YGDRLIMLHKGEVVLDVKDKEKENLTVEEILEKFEYAV"
BASE COUNT          861 a          391 c          329 g         1017 t
ORIGIN      
        1 cccagtgcca taatcgataa aattaaactt tgcgtcatta cagatataat acctgacata
       61 aataaaacac tcctttgaaa atatttttat aattttaatt atttgctaat tttgtgtata
      121 aaaaaaccac atagtgtaca aaactatgtg gtaaaaacac gaataagcca catagtttaa
      181 tctatgtggc tcttatatag ttaaatgaaa actttataaa caatatagcc aacatagata
      241 caatcccaaa attttaatag tcttaagggg tctgtatcta tgttaaaacg taggtacaaa
      301 ccctaaaaat agtggctata ataataataa tattttctat tgtttaataa agtattcata
      361 attatctctc ccttttaata ttttaattag tcagaaaatt atatgtattc tcgacttgtt
      421 ataaattata aattatacaa taataaaagt caactgctaa atttaattaa aatactaaat
      481 aatgtatact cagaatactt ttaaattaat gtattgtttg caaaaaacgt tatcctaagt
      541 tataagtgaa tgtggaaatt ttatcaaaat atttactaaa tatggaataa ctagacatgt
      601 atatcaatca aatatcataa aaaaaagctg tctggagaat attaaaattc ttttggacag
      661 ctcaagtagt gattagaaag ttatataact ttcataatca gtcgtcaata tagacgactt
      721 ttatggaaaa tgaataactt tatatgcaat attcaaattg ctacaataag cacaattttc
      781 aaagggttag ggtggataag agtacttatt atataaagtt ttcatattac ctacttcata
      841 ttacctattt tttgaaatta ataagtttta ttttgcagat tttgcatttt catctgactt
      901 tatatacttt atattatctg ctgataactt ttgcatgtca agtgctttta gtgtatcttc
      961 gttaattatt atttcagttt catctaatgt agtaactggt atatcgccaa cagattttcc
     1021 ttctaatact tcaatagcaa gttctccagc cttataacct agtttttcat aatctattcc
     1081 acaacaagct aaagcaccag aagatacaga accttcttca gaggctatta taggaatctt
     1141 gttttcatta gcaactttag aaactattgg catagaagaa actattaaat tgtcagtagg
     1201 gacatataaa acatctattt tgccaactaa actagaaata gcttggttaa cttcacttga
     1261 agtgctgatt cctttttcaa ctacttcata attatttttc ttagcataat catgtagaga
     1321 atcaacctgg atttttgaat taacttcact agtattgtat ataaccccta ttttctttgc
     1381 ttttggagtc aatgttttaa ccaattctaa tgttttatca attgaaagat aatcagatgt
     1441 accagaaaca tttccacctg gtttttcaag agatttaaca agtccagctt ctacagggtc
     1501 tgtaacagca gtcattatta ttggaatatc tttagtagca ttataagctg cttgtgcaga
     1561 tggagtagat atagcatata tcaaatcttt tttgtcggat acaaacttac tagcaatact
     1621 ttgtgtagta ggcatatcat tttgtgcatt ttggaaatct atttttatat tatctccatc
     1681 tttatagcct ttatcttcga gtgctttgat gaatcctttc tttgctttat ccagagacgg
     1741 atgttccact aattgactaa tacctatatt tttttgtttt ttgctatctg tttttttatt
     1801 ttcatttgaa gcattggagc catcattttg agaacatcca gtaagcattg atacaccaag
     1861 tactcctgct ataattaaac ttaatatttt tttacttttt atcattttat tttcctccct
     1921 taaaatttaa taattatatt tgttcatata ttcatatgat agattataaa tatttctgat
     1981 taaaactaag attttaattt cttatactgc atattcaaat ttttctaaga tttcttcaac
     2041 tgttaaattt tctttttctt tatcttttac atctaataca acttcaccct tatgaagcat
     2101 tatcaaccta tctccatatt gaagagcgtg tttcaagttg tgagtaacca taagtgtagt
     2161 tatatttttt tctcttacga ttttatctgt taactctatt acttcatttg atgtttttgg
     2221 gtcaagtgct gctgtatgtt cgtctagtaa taagacctta ggacttgcta agctagacat
     2281 aattagtgat agtgattgtc tttgacctcc tgaaagatat tgtactttaa tatctaaata
     2341 tttttttaag tccaaagaaa ttccttcaag taagtgttcc aaattattcg ttttatgacg
     2401 aagacatttt tttaagttca ataatttccc tttattaagt gctagagata aattttctct
     2461 aaccgtcata gaaggacaag ttccaaggct tgggtcttga aaaactctac ttactatttg
     2521 agttctttta tactcagcta aatttgttat agtagtacta tctaaagtaa tttgaccaga
     2581 agattctttt ataagccc
//
LOCUS       DAMJVX010000288         2589 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.295, whole genome shotgun sequence.
ACCESSION   DAMJVX010000288 DAMJVX010000000
VERSION     DAMJVX010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.295"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(219..1469)
                     /locus_tag="PC407_003584"
     CDS             complement(219..1469)
                     /locus_tag="PC407_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HDF2498988.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLYEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDSDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1501..2442)
                     /locus_tag="PC407_003585"
     CDS             complement(1501..2442)
                     /locus_tag="PC407_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDF2498989.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
BASE COUNT          778 a          498 c          241 g         1072 t
ORIGIN      
        1 tatttaaaac cttataaata tacaaataca attatatgaa ttttcatcct caattatagc
       61 atagaatatg actatctata tcctaaaatt atccatttct aaaaaaaggt aactattatt
      121 aagaatatca acttatagaa tttatattcc acatacgatt ttcaatcaaa aaaacaaggg
      181 ctaaaaagtg aattgtacac tttttagccc tttattaatt attcatttat ttcatgtaag
      241 aatagcccac atcttacttt aggttcaaac catgttgact tagctggcat tatcttattt
      301 gcatctgcta ctcttattaa atcttcaata ctagtaggat acattgcaaa tgctactttc
      361 atatcttcat taactctttt ttctaattct tcaagacctc taattccacc gataaaatca
      421 attcttttat ctactctagg attgtcaatt cctaaaatag catcaataac atagtcttgt
      481 aatatagaaa tgtctaatga gtcaacaggg tcttctattt ctaataaatt tttatttgct
      541 ttcattttat accacttatc acctaggtac attccgaaag ttcctttttt gtcaggttta
      601 tatttttctt ttccttctat ttcttctagt tcaaaatttt cttttatttt atttataaat
      661 tcttcctcgc ttaaaccatt taagtctttg ataaccctat tataatccaa aaccattaaa
      721 ttctcatctg gtgcaatcat tgctatgaag taattaaatt cttctttgcc agtataattt
      781 ggatttttag ctctcatttc tttggctaca ttttctgctg aagctgtcct atgatgacca
      841 tctgcaatgt atagataatc taatttttca aatttatcta ctaggttatc tattatatca
      901 tcactatcaa tcttccatac tgtatgctct atcttgtcat ctgttatgaa atcatataaa
      961 ggttcattat tatccataaa atcattgatt atatcatcta tcaccctttg atgtttgtaa
     1021 gttacaagta tagttcccgt attagcatca cagtatttta tgtgtttagt tctatcaatt
     1081 tccttttctg gtcgagtgta ctcatgtttt tttattgttc cattcaagct ttcatcaact
     1141 gatatacatg ctactatacc aacttgagct ctaccatcca tgatttgttt atatatatat
     1201 atacactctt tgtcatcttt tactagaatt tcttgttttc gaaactcata taaattttct
     1261 cgcgctttta aataaacttt ttcatcatat aaatctatat tttcatctaa atcaatttct
     1321 gatttatcta tatgcaaaaa tgaatatgga ttcccttctg caattcttct agcttcttta
     1381 ctattcataa catcgtaagg aagtgctgct actttatcta ctatatattt atttggtcta
     1441 atcgccttaa atggtttaat cgttgccatt actaatttcc tcctaattat acaaaatatt
     1501 ttataatgct acccctatta gattgttgaa gtcaagaaag aaatcttgaa tcacatttac
     1561 tatttcgtct cctattcttt cttgagcttc tactgtagat gccccaatat gaggtgtagg
     1621 agaaactctt ggatgattta ctaagtctaa atttaatgta ggttcttgtt cataaacatc
     1681 tatagcagct gctgataatt taccttcatc taatgctttt agtaaatctt tttcatcaac
     1741 taaaccgcct cttgcacagt ttattaaata agcccctttc ttcatcttat ttatttcttt
     1801 ttcagttatt actgccccct tattcttatc aaatggtatg tgtaaagata agaaatctgc
     1861 attttctaaa acttcttcca tatcacagaa tttataattg ttaaaacctt gcgcctcacc
     1921 cattatgtca gtatatataa cattcatacc taataattct gctctctttg caacttcttt
     1981 tgcaattctt ccaaaaccta taagtccaag tgtctttcca ttaatttctg tacctttata
     2041 cttcttcttc tcccattttc catctctcat agttacattt gcagtattta tgaatcttgc
     2101 aagaacaaat aattgaccta ttgtaagctc tgcaacagat atactgcttg catttggagt
     2161 attcataact tttatgccat tagcttgagc atatttaaca tctatattat ccagacctac
     2221 tccacctctt actatcaact tcaacttttt accctctaat gctttatcta ttacatcttt
     2281 agtaacttta gtagctgaac gtactacaag tacatcaaca tctttaagtt tatctcctaa
     2341 aacatctttt tcataaaact gctctatcac attaaaatct aactctctca attttctcgc
     2401 tgcttctttc tctattccat cagtaactaa tatattatac ataacaaact cctccaattt
     2461 ttattcactt aatttttcca atttttattt tttaaagtat tttcatcaat aaaaaacttt
     2521 acttaattta actttctgtt tatataattt tcaaaatatt tttttattaa agtcatatca
     2581 aattttcct
//
LOCUS       DAMJVX010000289         2527 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.296, whole genome shotgun sequence.
ACCESSION   DAMJVX010000289 DAMJVX010000000
VERSION     DAMJVX010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.296"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            358..1884
                     /locus_tag="PC407_003586"
     CDS             358..1884
                     /locus_tag="PC407_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2498990.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NNGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1912..2463
                     /locus_tag="PC407_003587"
     CDS             1912..2463
                     /locus_tag="PC407_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498991.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPYYSKKI"
BASE COUNT          992 a          214 c          450 g          871 t
ORIGIN      
        1 aataaatttt taagtaaata tttttataga gagattgtta tattacatat cagaaaaata
       61 ttaaacgaac taaatcaata aaaaagttgg tggctaacaa tttataattg ttagcacaat
      121 ataatcttat gtattgaaat ctagaatatc ctcattttta cttagcataa atactattga
      181 ttaagaaaat aatatagaaa ttataaatat atataagttt agtctggtgt ataaaacaag
      241 aaaactaagt gatttttcat ttagcttttt attaagctta ttttttatac tatttataat
      301 aaataaactc catgatggga aaactattac tatttaatat gtaaaggagt attttaaatg
      361 aatgaatcat attggttttt aaactcaagt ccaaaagaat ataacaaatt aggtgaaaat
      421 ataaaaacag attgtcttat agtaggtggt ggaataactg gattgacaac tgcatattta
      481 ttagcaaaag aaggtaaaaa agtagtgctt gttgaggctg ataaaatagg atatggaact
      541 tctggaagga atactggtaa ggtaacttgt cagcatgata tattttactc taagatagag
      601 aaaaaatatg gattagataa agcaaaatca tattataatg caaataatga agctttaaat
      661 ttagttgaac aaataataga agaaaacaat ataaaatgtg attttaaaag ggaaacttct
      721 tttgtattta cagaaaaaga ggatactata aagaatatta aagatgaata tcgtacatgt
      781 aaaaaaatag gaataaattg tgagtatcat gaaactatag aaaatattcc attagatata
      841 aagggggcta ttagttttac taatcaaggt caatttaacc caaagaagta tattgatgga
      901 ttggcaaaag cagcagtaaa tttagggcta aaaatatatg aaaatacacc agtagtagat
      961 cttgaaaaag gaaaaatatg tagggttaaa acaagagaag ataatataat agaagctgag
     1021 aatgttataa tttcatctca tagtccatgg tatgatggat tgaattttta ttttgcaaaa
     1081 gaatatgcag agcgtgctta tttaatggtt gcagttttgg aaaataaatt agcagatggt
     1141 atgtttataa gtatagatga cccatcaata acttttagac aatataataa tggaagtgaa
     1201 aatttactga tttttggagg aggagaccat aaagttggcc agggaggaac tgagaaagaa
     1261 atatttgatg atttagagca ttatggtaaa gaagtcttta aggtaaaaga ttttaagggg
     1321 aaatggtctg ctcaagataa tatgagtttt gacaatgttc cttatattgg ttatatcaac
     1381 aaaagagaag acaatattta tgtagctaca ggattctcta aatggggaat tactaatgga
     1441 actgctgctg gaataataat aaaagatttg ataataaata ataattctga ttataaagat
     1501 acttttaatc catccagatt aggtagctac ttttcaaaag attttataaa ggaaaatgca
     1561 aacgtagcaa taaattatgt aagtggaaaa ttaaaaatag gtagtggaga tatgcctaaa
     1621 aataatggag aaggaaagat tgttaatatt gatggaaaaa gatatggtgt atataaggat
     1681 gataatggag atttttatat tgtagacacg acatgtactc atttaggatg tgaacttaat
     1741 tttaacagtg aagaaaaaac atgggactgt ccatgtcatg gttctagatt tgactataag
     1801 ggaaatatac ttgaaggacc agctcttaag ccattaaaac tttatggaca tggtgataat
     1861 gatgttaatc ctaagttatt ataaaattat ttttaggaag gtaaagtgta tatgaataaa
     1921 cttttaaagc caataactat actattgtat atagtttata ttttatctgc cgtatttttt
     1981 gtagcaaaca aagaatatgg aagtctaggt ctagtagcaa ttagtttagt tggtactttc
     2041 attttgagtt ttataaataa gcaaactgaa agattattga gtaaagaatt atatataatg
     2101 cttgctttat ttataatgtt ttcatcattg ttaggtactt gttttaattt ttatgatata
     2161 aattattatg atgatttttt gcatttatgg tcaggtataa tatcttgtat tgtggctttt
     2221 tctataatta aatactttta tacacaaaga gagataagta aaatgagtaa agtgttttta
     2281 gttatatttt tatttatgtt tagtatggga gtagcgagtt tatgggaaat aggtgaattt
     2341 ttaatggata cttttgttgg gactactaca caggccgggg ggttagaaga tactatgatt
     2401 gatatggtag atgctttaat aggcacaatt ataactattc cttattatag taaaaaaatc
     2461 taatatatag ttttaaattt tttatattaa ttataatgga tttaattaat catgttgagt
     2521 ttcaagg
//
LOCUS       DAMJVX010000290         2502 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.297, whole genome shotgun sequence.
ACCESSION   DAMJVX010000290 DAMJVX010000000
VERSION     DAMJVX010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.297"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(<1..712)
                     /locus_tag="PC407_003588"
     CDS             complement(<1..712)
                     /locus_tag="PC407_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2498992.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLK"
     gene            complement(752..1099)
                     /locus_tag="PC407_003589"
     CDS             complement(752..1099)
                     /locus_tag="PC407_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2498993.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(1317..2273)
                     /locus_tag="PC407_003590"
     CDS             complement(1317..2273)
                     /locus_tag="PC407_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDF2498994.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTIYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIQAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
BASE COUNT          849 a          367 c          297 g          989 t
ORIGIN      
        1 gtttaagata tattattata tcttctggag ttctatcaaa tttgagttca aaattttcat
       61 cttcaaagta gggaatataa tataaaggtg ttttgagagc aggtaattta ttatataatt
      121 cttcaagaat atcgatattt ttaggggagt taaagaaaaa gcatttacat acgttcatta
      181 aatccacagc atctgaaagt gagttatgtt taatatctcc ttctacagaa aaaatttttt
      241 tcatattcag taaagatatt tgcttagata agattctatt attgtatgta atatgttgag
      301 atataaaagg ttgaatgtca atcatcatat taaataactc taagcattta cctgtatatt
      361 tatatactct aaatgaagtt agaaggccag ttatatcaaa atcaccccaa ttatatattg
      421 taacatcatc aaatttacaa aaccatttat aaaaatcatc cataacatat aaaagattag
      481 gagcattatt gatatctagt tggttcaatt tggttaaatt tttacattta tttgaaagta
      541 ttttattgta ttttggctta actaaagagt aataattatc tattgtatta aattcgttat
      601 caactaaaac tccaccaata gaaattaatt ccgcaccatt atattctttt ggtttatttt
      661 tattatcatc tagtatatta aattcaaagt ctataaataa cttgtatttc ataaatctaa
      721 agcacatcct caggtaaatt atttattgag cctaagcatc ataagaatca atagatataa
      781 tgactctaaa catcaaaggt tcattacaac ttttatttat ttcataattt aattcaaata
      841 aatcttttaa gatataattt ataagtattc tttcccaacc catttttaaa ttaactttag
      901 agtctattaa tatattttct atctttttaa atatttctat atttgctggt attatactga
      961 gctcaatatt acttttaaac gggttagact gtaaatctgt tataagtata ttttttaatt
     1021 cctctgtaag ttcagaaatt aaactatcac ctatgtattt ttttatgatt tcctctctaa
     1081 tttcttttaa atttagcaat attagtacct gctacttaca tagtagcttt tcctttcctt
     1141 ttaatactat tattcatttt tgaatcttat ttagatatat tttaagatta aaagtcttta
     1201 gctatataat tttttaccca tttttaactt atattaacat attacaggct tatatctcca
     1261 ccctgcttac attgaggatg gagtatattg cctattttga taaaaatgat aggttgttat
     1321 ttgcctattc ctttttctga taatttttct aacatatctt tggtcatttt atctaaatca
     1381 taactaaact taaagttcca ttcttttact gcactactac tgtctagaga gttaggccaa
     1441 gaatcagcta tactttgtct tacagggtct acatcatatt ctatcacaaa atcaggaata
     1501 tattttttta tagaatcagc tatttcttca ggagaaaagc tcatagcagt aatattaaaa
     1561 gcatttctat ggatgagttt atccgctgga gcttggatta aatccactat tgattgaagt
     1621 gcatcaggca tatacatcat atccattttt gtaccttctg ctatgtaaga tttatatctc
     1681 ttatttttta atgcctcata atatatatca actgcataat ctgtagttcc tccaccagga
     1741 ggcgtaacat aagaaataag acctggaaat ctaacacctc tagtgtctac tccaaatttt
     1801 gagtgataat aatcacatag taattctcca gaaactttag tcacaccata tatagtatta
     1861 ggtctttgta atgtatcttg aggagtcata ttttttggag aattttcacc aaaagcagca
     1921 attgaactag gtgtaaaaaa tttacaatcc aattctttgg ctatttcaag gccatttatt
     1981 aatccattca tatttaagtt ccaagcttct aggggtttac tttcagcaac tgcagaaagt
     2041 aaagatgcta gatgaattat agtatctaca tcatattttt ttgcaatttc aaaaaaacga
     2101 ttcttatctg taacatctaa tatctcaaat ataccagatt cacatactgg attaccttct
     2161 tttactctcc ttgaactagc gattacattt tgttctccat actcatttct aagctttatt
     2221 gttaattctg aacctatttg tcctaatgca cctgttataa gtattttttt caattttaca
     2281 acctcctaag ggtttaattt atgtagtact acttattttt attttagcaa atcttttgtt
     2341 taaatacaaa gaattaaaga taataatata cgacttaatt gaatgaagtg cagtaaattc
     2401 ctaaaaattt aaagttgaga tataatggtc tattgtataa caataaaaga ctagattaaa
     2461 ttattatata gattgtgtga ctaagtcaca gaaagtatag tt
//
LOCUS       DAMJVX010000291         2501 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.298, whole genome shotgun sequence.
ACCESSION   DAMJVX010000291 DAMJVX010000000
VERSION     DAMJVX010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.298"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            83..1597
                     /gene="glpK"
                     /locus_tag="PC407_003591"
     CDS             83..1597
                     /gene="glpK"
                     /locus_tag="PC407_003591"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2498995.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            complement(1711..2340)
                     /locus_tag="PC407_003592"
     CDS             complement(1711..2340)
                     /locus_tag="PC407_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2498996.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
BASE COUNT          929 a          287 c          464 g          821 t
ORIGIN      
        1 agtatgatta tttattgaaa tatgtgatat taaatttttc tttttaataa attaaatatt
       61 atatgataag gagaatatgg tgatggaaaa gaagtatgta atggcattag accaaggaac
      121 aaccagttca agagctatac tatttaataa aaaaggcgaa atagtgaaaa ttgcacaaaa
      181 agagttcaat caaatttatc caaaagctgg atgggtagag catgacccta tggaaatatg
      241 ggggtcacaa agtggtgtaa tgagagaagt tatagaaaca gcaggtataa gaccagaaga
      301 aattgcttca atagggataa ctaatcaaag agagactacg gttgtttgga gtaggtatac
      361 tggtaaacct atttataacg caatagtatg gcagtgtagg agaacatcag aaatatgtga
      421 tgaacttaaa aataaaggat tggaagaatc aataaaagaa aaaacaggtc ttttaataga
      481 tgcatacttt tcagcaacaa aagtaaaatg gatattagat aatgtagaag gagctaggga
      541 aaaagcagaa caaggagagt tattatttgg aactatagat acatggttaa tatggaattt
      601 aactcgtggg aaagtacatg taacagatta ctcaaatgca tctagaacaa tgatgtacaa
      661 tataaatgat ttagaatggg atgaggatat attacgagaa ttggacatac caataagcat
      721 gttgccatta gtaaaacctt ctagctatgt ttatggtcat acagatgagc gcatgttatc
      781 aggagctaaa atacctattg caggatgtgc tggagaccaa caagcagcat tatttggtca
      841 aaattgtgtt gaagaaggta ctgcaaaaaa tacttatgga acgggttgtt tcttacttat
      901 gaatacaggc agtaacatag ttaaatctaa gcatgggtta cttacaacaa ttgcttgggg
      961 agttgatgga aaagtaacat atgcattaga aggaagtata tttattggag gagcatctgt
     1021 tcaatggctt agagatgaat taaagataat tgaatctgca aaagatagtg aaatgtatgc
     1081 aaatagggta gaggatacta atggtgtata tgtagtgcca gcatttacag gtcttggagc
     1141 tccatattgg gatatgtatg ctaggggttc aattttagga cttacaagag gagcaaaaaa
     1201 agaacatata gtaagagcca cattggaatc aattgcttat cagacaaagg atgttttaga
     1261 agcaatgcaa aacgattcaa aattaaaact aaaatcatta aaagttgatg gtggagcaag
     1321 taataataat tttttaatgc aatttcaatc agatatatta aatgtagata tagatagacc
     1381 caaaatagta gaaactacag ctttaggagc tgcttattta gcagggttgt ctgttggatt
     1441 ctatattgga agaaatgaaa ttacttcaaa gtggtcagta gaaaaagagt ttaatccaaa
     1501 tatgagtgaa gagaagagat gtaaattata taaaggatgg aaaaaagctg tatcgagagc
     1561 tttaagctgg gaaaaagaag atgaattgga tatttaaaaa ttaatcaaag ttaaatttat
     1621 ttgaatttaa aaagagcttt atgtaaaata tgaagctctt tttttgttgt taattttgca
     1681 tttttatata atcatactaa attatataat ttatttttcg ttttcatata aaaacattgg
     1741 tatagaattg taaatgtgtt ggtcaccaac tactaagaga atgtctcctt ctaaaaattc
     1801 aatatatggt ccaggagata ttataagttc ttcacctctt ttaattgcaa ctattgtagc
     1861 acctgtattt tgccaaaatt tagtttgtgc agctgtttga ccaatatatt tgcaattaga
     1921 atttatggtg aactgaacgg gcactaaagg attgattatt tcaagtctgt tggaatagtc
     1981 taaaatttta tttatagtat ctaatatttc attttctaaa ttagatttag tatctattaa
     2041 attataaagt gttgatttat atgatgatat atttgtaata tctctatatt tttttataaa
     2101 agaagaagct ttgtctggag atagtacaat aacaccttta ccctttatgc ttttaacaac
     2161 gccaatgtcc tctaaaattt tgacagctct tcttatcgtt tctggagata cattgtattt
     2221 accagccaaa acagagcgtc caaatagtac tgaatcctct tgtatttcac ccgtgtatat
     2281 tttatttgca atatccaacg ctattttttg gtatattggc atcgtatagt ttttatccat
     2341 tgtttttccc ccaatacaaa atttatgtta aaaaaatata taatcttaac aatactattg
     2401 tataatttat aggtttatat gtctacaata tgtacaaata ttgtgcatgt tttaaaataa
     2461 tattttaaca tgcatttagt atacatgatt ttattatact t
//
LOCUS       DAMJVX010000292         2494 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.299, whole genome shotgun sequence.
ACCESSION   DAMJVX010000292 DAMJVX010000000
VERSION     DAMJVX010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.299"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(281..1048)
                     /locus_tag="PC407_003593"
     CDS             complement(281..1048)
                     /locus_tag="PC407_003593"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF2498997.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKVAVMPQNERDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            1854..2486
                     /locus_tag="PC407_003594"
     CDS             1854..2486
                     /locus_tag="PC407_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2498998.1"
                     /translation="MTIPNSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT          937 a          362 c          272 g          923 t
ORIGIN      
        1 tatcttctac tttagataaa acagttatta tgaaaactta ctatctataa ataatagaaa
       61 tgctagtata ccatctataa tctaatgtct agtaggtttt aatggcatat tttaaatatg
      121 ccatagttga tttataagat tttctatatc actttcaaaa tacacactaa atagttgctt
      181 ttcagatata attgcccaat ttaaatttta ctatctgaaa agttttttat atttaattta
      241 tatcttcaaa aataccgtct aatatttcta tataattaat ttaattaata ctcttatgta
      301 atagattaag tactgaattt aactctttaa atgatatttg ccctggtgct gaaacacttt
      361 ttgcagctcc aaaagtcaat gcagaaccaa atatttcacc acacaatcta cttataacac
      421 ccattcctga cattgacatt gttattattg gtctatctgc ataaatttta aacatttcat
      481 ttgtagcttc aagtagcact aacacatctc tttcattttg cggcataaca gctactttgg
      541 gtaaatctgc gcccaattcc tgcattctac ataaacgaga tacaatctct tctttttttg
      601 gagttttatt aaaatcatga ttagatataa ttactttcac ttctttttta tgagcaaaat
      661 taactacttc atctatgacc tcatctccca taaataactc tacatctatt aaatctacta
      721 aacctgtatt agaaatttct ttattcaaag tagtataata atctcttgat attaattttt
      781 ctcccccttc tactacactt ctaaatgtga ataataaagg gatatcatgt atataacttc
      841 ttaattcata gagaacttcc ttaacttctt taatattttc tacattctca aaaaaatcaa
      901 ctcgccactc tataatatcc aaacatgcat cttttaactc tttagcctct tttataatat
      961 cttttttatt tttcccaata attggtacac aaattttagg tctaccttct ccaatagtaa
     1021 tattttttac ttgaactttt cttttcatat tctcctccat aatcagaaat tctataggac
     1081 tttaaattct attttctgca taatttaatt atacattaaa aatactatac attataaaaa
     1141 tactaattta aaaataattt ttattctaat tcttaaaaca atcccacata ttttagaaaa
     1201 ggtactctat aatatacaaa cacttctttt ttacctaaat ttattttaac attctataat
     1261 tttcacaata ttctttaaat tttactcata taaacaatat tattctggat gctaatagaa
     1321 ttaatctata aatcatagta taattatgca gaaataactc gaatttcact acttataaag
     1381 agattgaata ttaatgaatc taaatcattt gcattacttc agagttttga ctaaagttga
     1441 acaccataca aaagtagcta ctcaattgtt tataccccaa ccaagcttaa gtaatgttat
     1501 atagattctt gaaaaagaat ttggtatctt tttatttgaa aaacaaggta gaaatgtttc
     1561 cctcactaaa tacggaagtt tttttctaac ctatgttgat aaagcattag gttaattaga
     1621 aaaagtttac atataaaata tattaaattg ttgcaagttt tattgttttg atatacaatc
     1681 aactaaacat ataatcttta taaaatacta tacagttaaa agtctgtttt aaaaaaaaat
     1741 gtataaatta taaatcaaaa tatagatact atcaaggagt gattctgtga agagatgtat
     1801 tgtagtagtg gaatagtcaa aaacataata tatattaaag gagagattta aatatgacta
     1861 ttccaaattc aaataaggta tatccaagaa gtaatgatta tcaaactgta tatcttaaaa
     1921 atgtaataac aagggaaaat atagaagttg gagattacac aatatataat gacttttata
     1981 atgacccaag agattttgag aaaaacaatg tattatatca ttaccctata aacaatgata
     2041 aattaataat tggtaagttt tgctcaatag catgtaatgc aaaatttcta atgactagtg
     2101 gaaatcactc aatgaaatca aaatccacat atacttttcc catcttttat gaagaatgga
     2161 aactcgatat caaccatata acggacgctt gggacaataa gggtaacatt ataattggaa
     2221 atgatgtgtg gataggttat gattctataa ttatgtcagg tgtaaatatt ggtgatggtg
     2281 caattattgg aacaagagca gtagttacta atgacgtctc accgtattct attgttggag
     2341 gagtacctgc aaaaattata aaaaagagat ttaataatga tacaatttca aaattattag
     2401 aaattaaatg gtgggattgg ccatataaaa aaattcaatc aaacattaag catattcaat
     2461 caggaaatat tgataaatta gtttaatatt ttat
//
LOCUS       DAMJVX010000293         2393 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.300, whole genome shotgun sequence.
ACCESSION   DAMJVX010000293 DAMJVX010000000
VERSION     DAMJVX010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.300"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            332..2227
                     /locus_tag="PC407_003595"
     CDS             332..2227
                     /locus_tag="PC407_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HDF2498999.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKSETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         1046 a          241 c          445 g          661 t
ORIGIN      
        1 ggaggtagat attaatttag tgatatatat ttaagtaata agagcaaagc aaaaattagt
       61 gcatagtagt atataaaaat ggagtttgaa tacttttgtt agtatatgat tgtttagtag
      121 aaaatcacta taaaataggg atttctatct ttgagcataa ttatagagag ctaagtaggg
      181 ggaaggggtt caagaagata ttattagtgt ttgtgttagt aaattatgta aacacataaa
      241 atatattgaa aaataatatt atatataata ttatttatgt gtgggaaata cgaatataaa
      301 aaataaaaaa taatattcgg aggacataaa tatgttaagc aacaaaaaga gaagtatggc
      361 aatagtaatg gcaggtgcta cagttatgag tgcagcagca ccaatattcg cagacaatac
      421 tgtgacagaa aatgttgata aaaattacac agtaagtgcg aaagattctg ctaaactaat
      481 cgaagaggtt agaaaagcat tagaagttaa gtttgaagat acaaaagcag gagcaaatgt
      541 aaatgataga gtttacgata taaaagtaga caatgtaaac ttaactaatg caactcaatt
      601 acaaaataaa ataaactctt taacagaagg gcaaagttta aaagttacta ttcaagataa
      661 aggacatcaa gtgttaggtg gaaaagtagt tgactataaa attgaaaact acaaaactgc
      721 tcaagaaata gtagatgcag ttaatgctta caatgcaact ttagcagaag attctgataa
      781 caagttaaca gcaactataa aatctacaaa tacagttgaa gtaaaaagag caaaagattc
      841 agcaaatgtt attacattaa atgtaggaga ccaacattta gatttctcaa aagtgataac
      901 atcagaagaa ggtacttttg aaggttatga aaagagttat agtgacatag attcaaaaga
      961 attacatacg ataacagtta aaaacgcaga tttacaagac atatcagcag aagaattatt
     1021 tgatggaatt agattaacta cattaggaag agaaatagtt aataaagtta aaaatggata
     1081 tgctttaaca tttgaaaatg aagcaatcct tactcaagaa caagaagata gtgatgataa
     1141 agataaacca gaaaaatcaa gctttgatat agttttaagc aaggcaaatg aaaagtcaga
     1201 aacaataagt gtttcaagta aaaaccataa gttagttaga gacttacaca aagtattaac
     1261 tgatgtaaaa gatggaaaag aattaaaagt agaggtttta tctggtgatt caagatttac
     1321 aacagcagta gaagttagta aagagagatt caaagatgga gaagcagaag ctataatttt
     1381 agttggagaa gatgctatag ttgatggatt agcatcagca ccacttgcct ctcaaaaaaa
     1441 tgcaccaata ttattatcta aaaaagattc actaccatca gaaatagaag ctgaaatatt
     1501 aagagtactt ggaagtaatc tatcttctaa gaaaatatat atagtaggtg gagaatctaa
     1561 agtatcaaaa gaaactgaag aaaaactttc taaattaggt gtaagtaaag ttgagagagt
     1621 ttctggagaa gatagatttg aaacttcttt agaaatagca aaacaattaa aagatacatt
     1681 taagactgct tttgtagtag gtggaaatgg agaagctgat gctatgagta tatcagctag
     1741 agctgctcaa tttggtgctc caataatagt tacaggtaac gaattagatg caaatgctga
     1801 aaaattatta aaaggaaaag aattagaaat agtgggtgga gaaaattctg tatcaaaaga
     1861 agttgaagac aaattagttg atatagattt aaataataaa gttgaaagat tagctggaga
     1921 aaatagaaaa gatactaatg ctaaagtaat caataaatac tatgcaggtg caactaaagc
     1981 atatgtagca aaagatggtt atgtaggtgg aaatggacaa ttagttgatg cacttacagc
     2041 agcaccactt gcagctagtt caaaggctcc aatagtatta actacagaag aactttctaa
     2101 atcacaagaa gaagtagttg agttaagact taagaatgct actaagttag tacaaatagg
     2161 tgaaggaata gctaaaaacg ctattgaaaa aatagcagaa aaaataaact tatttactaa
     2221 gaactaataa gatataggaa ttaatattaa aagtttattt agataatata tgattataca
     2281 atgcgttaat atgtaagaca tagtgtagat attataaaat atacaaataa caagataaaa
     2341 taataaaaat aaggtgtctc atttagaatt agattgagat accttatttt att
//
LOCUS       DAMJVX010000294         2374 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.301, whole genome shotgun sequence.
ACCESSION   DAMJVX010000294 DAMJVX010000000
VERSION     DAMJVX010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.301"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            40..282
                     /locus_tag="PC407_003596"
     CDS             40..282
                     /locus_tag="PC407_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2499000.1"
                     /translation="MQNSMKQEPCDFSHGRFRFKTSDNRVLIPVVIFLGSIVTALCDL
                     ISRVILSPVELPISTITSLLGAPIVVMLLVKRKDRA"
     gene            285..1481
                     /locus_tag="PC407_003597"
     CDS             285..1481
                     /locus_tag="PC407_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2499001.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(1601..2326)
                     /locus_tag="PC407_003598"
     CDS             complement(1601..2326)
                     /locus_tag="PC407_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2499002.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGANFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTPFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT          893 a          279 c          388 g          814 t
ORIGIN      
        1 ggggatggcc tgtggagaat tagtaagaca tattttagta tgcaaaattc tatgaagcag
       61 gaaccctgtg actttagtca tgggaggttc agatttaaaa catcagataa tagagttttg
      121 ataccagtag taatattttt aggcagtata gttacagctc tttgtgattt aatatcaaga
      181 gtaattttat caccagtaga attaccaata agcacgataa catctttatt aggagctcca
      241 attgtagtaa tgttattggt gaaaaggaag gatagggctt agacatgttg aaaactaata
      301 atttaagtgt aggatacaat aacaaggtag ttataagtaa tataaatgta gaagtaaaaa
      361 atggagaaat actatgtctt ttgggaagta atggagctgg aaaaactacc ctattaaggt
      421 ccttatctaa acttatatca ccaataaaag gtgaaatata tttgaatggt gtaaacataa
      481 attgtatatc aagaaaagca ttatcaaaaa aaatggcttt agtacttaca aatagattat
      541 taggtgattt aatgactgta caagatatag tcaatatagg aaggtatcca tatacaggat
      601 tttttgggag tctatctaag aaagatttaa taatggtaga tgaagcattg gagtctgtag
      661 atgccttaca tttaaagaaa agatattttg atgaactaag tgatggagaa aaacaaaagg
      721 tgttggttgc aagagctctt gttcaagaac cagaaataat tatattagat gagcctacaa
      781 ctcacttgga tataaaacat agattggagc tcataaacat actgaaaaaa ttaagtaaag
      841 aaaagtcaat atcagtaata ctttcattgc atgaaataga catagcatta aaaagttgtg
      901 ataaagttgc tcttataaaa aataataaag tcatagctta tggtcagcct gaagatgtag
      961 tagatgagga tattataaat tcgctttatg aattggatga taaaaacttt aatagtcttt
     1021 taggttcagt agaaattagt aataaatcta aaaatgaagt ttttataatt ggtggtgggg
     1081 gtaaagcaac accaatttat agagctttta ctaaaaaagg tataggtctg tattctggaa
     1141 taatacatga aaatgatatt gattatgaaa ttggtagaac tatggggata aagatgttta
     1201 cagaaaatcc atttgagccg ataagtgatg agagtttcga tttggctatt agaaatttaa
     1261 atgattccaa gattataata gatacaggat ttagtgtagg agagacaaat aagaggaata
     1321 tagatattat aaaagaggct ttaaaactgg ataaaaaagt ttattctttt agaaatagag
     1381 atgagagtaa gaagtattat gacagtttgg atagtaagat agaacatata gataaagttt
     1441 ctcaaataat caatagtgca gatataaata atttattata atttatgaaa taaaatgtac
     1501 ttatgtgcta tagaaaaaag agtatagatt ataatttttt tgaaattaca atctatactc
     1561 ttttgttatt ttgtttatat atttataaaa ctttagcatt ttatagataa ccaatagctt
     1621 cctctctagt taaaaaaaag tgaatccctg tagtcgattc tttccatcta ttttcattaa
     1681 aatcttcagc tacaatcgtt tctcctactc tatatataaa attttcatca gcatatgcac
     1741 gagcactttt aaaagattct ttaccatcta tactagtaat tgacagtact tttgccttat
     1801 cacatctaca agcatttgat gtagcagaca ctctttttgc gtcgctgggt attaataatt
     1861 gtaccattct gaaattaaaa cattttttat agcctataaa tgctcctgtt tcaggacaat
     1921 acaatttaaa aaaaggggta ctttcatctg atattactcc atttaaagta gcatgttcta
     1981 aattggaatt ttctaaatta gctcctctaa tatctgagtt agataagtca caaaatctaa
     2041 aatttgcacc acttaagtct gcatttttta atatagaatt ttttaatgaa caatccgaaa
     2101 gataagaatt attaaaaata gagttttcta aatctgcttt ttctaaattt aaattgataa
     2161 ggtcagacca cgaaaagtca atattactta agcagtatcc tcttaagtcc atattttcta
     2221 atcttaattc ttttaaatct gccttttctc cagcattatt gtttttaagc cataaataat
     2281 gtttctcaag tgttttttct aattcttgtt tagaaatagt tttcattaaa atcaccttca
     2341 taaatttatt tttatatatg tagtagcaaa atag
//
LOCUS       DAMJVX010000295         2334 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.302, whole genome shotgun sequence.
ACCESSION   DAMJVX010000295 DAMJVX010000000
VERSION     DAMJVX010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.302"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            176..1627
                     /locus_tag="PC407_003599"
     CDS             176..1627
                     /locus_tag="PC407_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2499003.1"
                     /translation="MNFLNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1847..2308)
                     /locus_tag="PC407_003600"
     CDS             complement(1847..2308)
                     /locus_tag="PC407_003600"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDF2499004.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGRYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
BASE COUNT          793 a          270 c          401 g          870 t
ORIGIN      
        1 gtttgatttg aatataaaac tgcaatatat taaaaaaatt attcatttta aaccttgtaa
       61 tttgatttta taaatattat aattaaatta atgaattttt aacaaggaat tattcatttt
      121 tttgatttta agatttattg agaaaacaaa actaaatagg ggggtaatta atataatgaa
      181 ttttttaaat ggtattttaa cgagtattaa caatgcagta gtatttgtaa acaattactt
      241 atggtcgtat atcttaatta taatgttagt tgtaattggt atttacttta ctattaaaac
      301 aaattttgta caattcagat attttataga aatgtttaga cttctgggtg atggtacagc
      361 gaataaaaat gcaaaaaagg aaggaaaaat ctcttcattt caagcatttt gtataagtac
      421 atcttcaaga gttggaactg gaaatatagc tggaatagca attgcagtgg tagcaggggg
      481 acctggtgct gtattttgga tgtggcttat agcattaatt ggttctgcat caagctttgt
      541 tgagagtaca ttagctcaga tttacaaagt gaaagatggt caagctttta gaggtggacc
      601 agcgtattat atggaacaag gtctaaataa aaaatggatg ggtattttat tctcaatact
      661 tataactata tgttatggtt ttgtatttaa tgcagttcaa gcaaatactg tttcacttgc
      721 atttaataat gcatttggaa taagtaaaat ggctatggga ataggtcttg gaattttaac
      781 tgcaatagtt atttttggtg gagtgcatag agttgcaaaa gtttctgaaa taatagttcc
      841 tgtatttgca ggattatata tattagttgc tttggctata gttgtaatga atataacaga
      901 gataccaagt gttatagcat tgatagttga aagtgcattt gattttaaag gaatggcgat
      961 tggtacattt atgggagttg taatgactgg agtgaagaga ggtctatttt ctaatgaagc
     1021 aggtatgggt agtgctccta atgctgctgc aacagcacat gtaactcatc ctgtaaaaca
     1081 aggtttaatt caatctttag gtgtttttac agatactata ataatatgta gttgtacagc
     1141 attcatggta ttattatatt caggttatag tagtagtgga gcaactggaa ttgaattagc
     1201 tcaagaagct ttaactcatc atataggacc agttggaaat atatttatag cagtttgtat
     1261 attcttgttt gcatttagtt ctatagttgg taattactat tatggtgaat caaatatgga
     1321 gtttatgagt ggaagtaaga ctaaacttaa tgtatttaga gtttttgttg taggcatggt
     1381 tttatttggt tcacttactc aagtagatgt agtttggaat cttgcagact tatttatggc
     1441 attgatggcg ataataaact taatagcaat agcactttta ggaaaatatg catttattgc
     1501 tttaaaagat tatacaagtc aaaaaaaatc aggaataaaa gaccctgtgt ttattgcaga
     1561 tgaaatagaa ggcctagaaa aggtatcaga gtggcatagg gaattatctg aggaacattt
     1621 aggataaaaa atagtataaa attataaaac cattttgaaa gactagagta tgtatatgct
     1681 ttatatgaat ataatattga attaatttat attactggaa tttttaattc cagtaatatt
     1741 tttttattat ataatttagc tttattaaaa gtttttatta ttaaaaatag cttatttatc
     1801 gtaaagtgaa tgatattggt tttttaatta ttatgaatgt tggtatttaa tttaaagact
     1861 taaataaatt tgccatttct agcgcattca tagcacaatc ataaccttta tttccagctt
     1921 ttgtacctgc tctttcaata gcttgttcaa tattttctgt agttacgatt ccaaatataa
     1981 caggtaattc actgtctaaa gatacttttg ctatcccttt agagacttca ctacatacat
     2041 aatcataatg agaagtagcc cctcttataa cacagcctaa acagattata gcatcatacc
     2101 taccactttt agccattttt tgagcaacaa gaggaatctc aaaagctcca ggtacccaaa
     2161 ctatttctat attttctgga gaaacgtcat gtcttaaaag acagtcttca gctcctgata
     2221 aaagttttga tgttataaat tcattaaaac gagaatttat tatacctatt tttaaatctt
     2281 ttccaataag ttttccttca tatatcattt ttatttcctc caattttatt tatg
//
LOCUS       DAMJVX010000296         2269 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.303, whole genome shotgun sequence.
ACCESSION   DAMJVX010000296 DAMJVX010000000
VERSION     DAMJVX010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.303"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            102..2174
                     /locus_tag="PC407_003601"
     CDS             102..2174
                     /locus_tag="PC407_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2499005.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLSHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYASIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKLLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSKKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNCLSTISPVVFTNLEQNQKLSIKKGITTAKEENRVSIL
                     RYDIEKIG"
BASE COUNT          835 a          250 c          437 g          747 t
ORIGIN      
        1 acataagttt aattttagaa ttaagtatac taatgtaact ttttataaac ttaagtcttt
       61 tttatttaaa aaaatataaa ataataagga gagaaaagta aatgaaaaaa gtatttggtg
      121 ttttacaaaa agtaggaaaa tctctaatgc taccagttgc attacttcct gcagcaggta
      181 tattacttgg cgtgagtaat gctttagcag gagacgcatt gttgtctcat tttccagctt
      241 tggcgaatcc tacatttcaa cttataataa gtataatgga aagttcaggt caaattatat
      301 ttaataactt accacttata tttgcagttg gggtagctgt tggtttaact gaaggagaag
      361 gtgttgcagc attggcagcc atagtaggtt ttttaattct caatacatct atgggtgtta
      421 tggcgggtgt tacagaagat atggtagaca attctatgta tgcaagtatt gttggaatac
      481 caacaattca aacaggggta tttggtggta ttattatagg gatagtggct gcttatcttt
      541 ataaaaagtt ttataaaatt gaattacctc agtatctagg tttttttgca ggtaaaagat
      601 ttgtaccaat cgttacttca ttagttgcta tagcaatagg aattatattg tcttttattt
      661 ggcctccaat acaagaggga ttacttagtt tctcaaaaaa tgttatagaa gcaaatcaaa
      721 cactagcagc atttatggaa ggttctttgg aaagactatt aataccattt ggtttgcatc
      781 atgttttcca taatccattt tggtatcaat ttggagaata tgtaaataaa gctggtgagc
      841 ttgtaatggg tgaccaaaag atattctttg ctcaacttaa agatggagta aatttcacag
      901 caggtacttt tatgacaggt gcatttccat ttatgatgtt tggtcttcca gcagcagctc
      961 ttgctatggt gcatgaagca aagccagaaa agaaaaaatt tgtagcaggt ataatggcat
     1021 cagcagcatt aacctctttt ttaacaggaa taaccgagcc aatagagttt gcgtttttat
     1081 ttgttgcacc agtattattt gcaatacatt gtttattttc aggtttatca tacgcaatta
     1141 tgcaattatt aggagttaaa attggattca cattttcagg aggattaata gatttcttat
     1201 tatttggtgt tcttcctaat agaactccat ggtacttggc tatcttggta ggtattggtt
     1261 ttgcagtatt atactatagt gtatttagat ttatgataag aaaatttaat ttaaagacac
     1321 caggaagaga agatgagtca gaaactaacg ctgaagtcat tgaaatgtct aagaatgaat
     1381 tagcatcaga gattttaatt gctttaggaa ataaagacaa cttaaagagc ctagatgcgt
     1441 gtataacaag acttagattg gttgtaaatg atataaataa agtagataaa gaaaaattga
     1501 aattacttgg agcagcaggt gttatggtta ttggcaacaa tgttcaagct atatttggac
     1561 caaaatcgga ttcaataaag tctgatataa aagatataat agagggaaaa acaaaggttg
     1621 aaagtactgt tactaaaaat gttgataaaa ataaagtgaa aaaaaatatg gaagttgcca
     1681 atatggaaaa tgctttagta gaaaagattg attcattaac aaatggagag atattagata
     1741 tagaagaagt gccagattct gtattttcaa gtaaactgat gggggatgga tttgcaatta
     1801 aatctagtga tggattgatt tattcaccag ttgatggaac tattggagtt atatttccaa
     1861 ctaagcatgc aataataata aaatccaaaa aaagtggagt agaaatactt atacatttag
     1921 ggatagaaac tgtaaattta gaaggaaatg gatttgaagt atttgtgaat gtaggagatg
     1981 aagtaaaagc aggagaaaaa ttagttaaaa tggacttaga atatattgaa aaaaattgtc
     2041 tatcaacaat aagtcctgta gtatttacga atttagaaca aaatcagaaa ttaagcatta
     2101 aaaaaggaat aactacagca aaagaggaga acagagtaag tatattaaga tatgatattg
     2161 aaaaaatagg atagctatat gatagtataa ctaaaagtta tgtgagattt aataaatata
     2221 taaataggtt cagttaacat gcaatttatt taatatatat tttaagtaa
//
LOCUS       DAMJVX010000297         2263 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.304, whole genome shotgun sequence.
ACCESSION   DAMJVX010000297 DAMJVX010000000
VERSION     DAMJVX010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.304"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(135..1427)
                     /gene="brnQ"
                     /locus_tag="PC407_003602"
     CDS             complement(135..1427)
                     /gene="brnQ"
                     /locus_tag="PC407_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2499006.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLI
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIKFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          760 a          382 c          246 g          875 t
ORIGIN      
        1 cctccaaaaa taaatttatc aaatgagatt aataatgttc ttttctccac aataatttaa
       61 taattctttt aaatataaaa aggaattgcc atataattta aaggcaattc cacaattatc
      121 aattagtttt attattatgc agttttactg cttttagatg gaataaagtt tcctattatt
      181 cctgctatta caactggaac tatccagtta aatcctaatg aagcaaatgg taagctatta
      241 actagaggaa tttttacacc aaaacttgtt gcaactgtta atacacttac taataatgcc
      301 atgtatgcag cacatttgaa cacgttgtca tttttaattt tattactaaa taatgctagt
      361 ataactagag ttatagttgc tggatatacc atacttagta taggagaaga gaattttatt
      421 attgtactta ctccaaagtt tgatatgatt gcactaaata cacaaactac tataactata
      481 tgctcatact ttaattttcc atctgtaagt gttgagaaat attgaccagc tgctgaagaa
      541 agacctatag ctgttgttaa acatgctaat gcaactataa tagccaataa aattttacct
      601 gtagttccaa gtaaagctgc tgttattgca actattaaag ctgtttgttg aacatcttgt
      661 ccatattgtt gagacacagt tgctccaaga taacataatc caccataaac taatgccatt
      721 cctattactg caattacacc tgcacttatt gtggctttca tcttttcttt ctcacctgta
      781 taacctttag ctattactga catgattata attgatgcaa atacactagc tgccaatgca
      841 tccattgttt gataaccttg agttatacct tctgcaaata cattttgaat taaagaactg
      901 tctcttattt ctcctaatgg agatacgata cctttaacta ttaatactgc taaagctata
      961 agtaaagctg gagttaatat tgacccaact atatctacaa cttttgatgg tcttattgtt
     1021 aaaaccaata ctagagcaaa gaatactata gagaatatta ctggacttat tccagaacct
     1081 attgtaggca atatacccat ttcataagtt gttgctgctg ttctaggtat tgctaaaagt
     1141 ggccctatac agattaccat tgcacatcct aatgcaatac ttaatccttt gcctgctctt
     1201 gagaacatct tcgatgtgtc tccattgaac ttagttgctg ctataactgc taataatgcc
     1261 aaaccaccat ctgcaaaaag gaatcctatg aaagctatta gccaactact tccagataaa
     1321 actcctagat atggagggaa gattaagttc cctgcaccaa aaaacattgc aaatagtgca
     1381 aatccaatta caattacatc tttaaaattg atttgtttct cacccattta aagcaccccc
     1441 atttgaattt tactaccttt tactaaaaaa taagtccaat ctcttcatta aatgaaaacc
     1501 ttttgacgag attttattta attatacatt tttgacttaa atttgtcaat tatgtttcat
     1561 aatttataaa atcttcacaa tatttaatac aactttctta attaaacaaa atcaacctgt
     1621 atactttaaa catagttggt tttttgtttt ttctctaatt ttccttgata aaattaacta
     1681 ttcagaacaa ttaaaatatt aagacttttt agattattcg ttttattatt aagttgtatt
     1741 gctttatata ctgtatatat cactctcctt tttacattca tataatcttt ttaattcaat
     1801 ttaaatttta aatattttgt gtttattata taatctgaaa aaaaagcagt caatagcatt
     1861 tttaattttt ttaattttca aaattatgat tcttaaaaaa tcgttttcct tttatcataa
     1921 cacctattaa cgttaattct tgtagtattt tgtatttatt ttgctaaatt gacagttaac
     1981 tttattcaat ttttattcta ctcacgtaga tttacctctt gtcttttagt cttaaaacca
     2041 ttttttctga aaattcctta ttatgattta tcaacttatt tttcttcata gcaattactt
     2101 tctaacttcc aacttactta aactttgcag ttgctagtat tcacgtttac atacttatat
     2161 gtcataatta actaaaaatt cttaatatat ctctcaatga aaacaattta ctcatttatt
     2221 taatacattt atataatcat atgtcttaaa ttttgtttta tta
//
LOCUS       DAMJVX010000298         2247 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.305, whole genome shotgun sequence.
ACCESSION   DAMJVX010000298 DAMJVX010000000
VERSION     DAMJVX010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.305"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(79..993)
                     /locus_tag="PC407_003603"
     CDS             complement(79..993)
                     /locus_tag="PC407_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HDF2499007.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKDITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            complement(1825..2007)
                     /locus_tag="PC407_003604"
     CDS             complement(1825..2007)
                     /locus_tag="PC407_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499008.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
BASE COUNT          821 a          281 c          284 g          861 t
ORIGIN      
        1 tataaaaaat aaaggtacag ttaactgtac ctttatttta tctaaatatg caaagattat
       61 tttaaaagta atttttaatt acatgttttt ataactctcc aatattctaa tataatgatt
      121 tgcctctctt aaaacatggt ctgctaaaag tggtaatatt atagatttta tcttacactg
      181 ttctattcct gatgctcctg cttctttaaa atctttaaac tcaacagttt catttagagt
      241 ttcttctgta atgttcttga tattagaatc agtcatttcg tttgtttttt caattaattc
      301 attaaatttt atagcaaaat catttgaagt atttattagt tcaccttctg aggggtctag
      361 taatcctctc ataaacagag catgttccat cataatatgg tcccagaata attctatttc
      421 tttagcgttt tttgactcaa tatctatttt attttcaagg tcaactacat aagaacgata
      481 taaatttgct tcatgtatta tatgttcaag aagtagaggg tagtttgatg taaatatagt
      541 acatgatagt acaccatcta agactctttc tttaaaatta ataagcccat caagtaatct
      601 tattgcatca gagttaagtt gagctacata gttcactaag tcttgaccaa cttgtgggtt
      661 tacaccactt tgtagattta attctcttgt agtgatgtct ttgtttattt ctatccctgt
      721 aaactcttct gttttttgtt ctgcaactga tgtgagagta gttacaagct cttctgaata
      781 taatatatca ggtctaatta taccattact agcactaaca gtgtatgata ataaatcttc
      841 aaattgcttt ttatagtggt cagcttccat agcaagatta taatttttat ttgtgaatcc
      901 tgcttctaaa aaaagagcat gctctttcat aattcttgaa aaaaataaat gtaattctag
      961 tgataaaata acatattttt gattatctat cataatttcc tccctttaga aagtatccta
     1021 tttataatac tttattcgta atatttaaat ttatgtatat tataaataaa ttaactaaat
     1081 tcaattgcta ctaatatcaa cagtttttat taagttatgg gcgaaaaaat tatcttactg
     1141 tgtttcttat tagatataga tataatgatt taaggtactt gttggctatt atttattaag
     1201 agcacaagta atactagaaa tttttatgag aattatgtaa tttataagta ttattttgat
     1261 acaaaacaaa tattttttgt gaaacaagaa cttttatagc aaaaaatgaa taaatatgaa
     1321 gcaatcataa ttttatacca caacaattat taattctaat agctgcactt aatgttttag
     1381 gtatgggatt taaaaagtat aaaaaattaa gtgataaata tattccatta tattgcttgt
     1441 gcttggagta gtattttctg tatggatgtt aggatttaat ccaacttcta ttttacaggg
     1501 gattctatgc tggggtgttt caattggatt aaattaagta tgcaaacaat ttcaagacaa
     1561 tgataaataa ttggatgtgt gataggaata tcaacaaaaa acattattaa aaagaattat
     1621 tttatttata aaataaaaca agtaataaga ataaataagg agcaactaaa atgatttaaa
     1681 tatactagaa ttaaaaagag aatgtaaagt tgtctagtaa tggaggtaaa atattttaaa
     1741 cgataagcta tgaaattgat atattaaaat gggtaagaag aatattatat aggattcttc
     1801 ttacccattt taataaattt aattttatgc caaaccacat tcgcatagta gaatcatcac
     1861 tctatggaga cataactcta caacatcttc tccatatttt ttaataaaat caccaatttg
     1921 agtttctatg aatgaatagt attcttttaa actagcaata tatgcagtca ttatatgaac
     1981 acattcatca atcttatctt taaacacaac taatccccct aaaaaacgaa aaataaaaat
     2041 attcagtaaa tagcagatat tatttcactc ataaatctat atagtatgat atattgctta
     2101 gatatatttt acaagtggat atgtaaaact ggttattata atcgcaaaac tggataaact
     2161 ttcattctta aaagaaaatt tatccagttt aaagtttaat tatgaatatt tgagttagta
     2221 gatatttaat ataaattaat ataagtg
//
LOCUS       DAMJVX010000299         2159 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.306, whole genome shotgun sequence.
ACCESSION   DAMJVX010000299 DAMJVX010000000
VERSION     DAMJVX010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.306"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            83..>2159
                     /locus_tag="PC407_003605"
     rRNA            83..>2159
                     /locus_tag="PC407_003605"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          645 a          428 c          610 g          476 t
ORIGIN      
        1 atctaatttg taatatataa agctgataac tttttaaaat tatcgaagtt gatagcttct
       61 aatctatcaa acctttttaa ctggtcaagt tattaagggt gcagggcgga tgccttggca
      121 ctaggagccg atgaaggacg tgataagctg cgataagctt cggggagttg cacgtaaact
      181 ttgatccgaa gatttccgaa tgaggaaact cacttagagt aatgtctaag tatcattaag
      241 tgaatacata gcttaatgag gggaactcag ggaactgaaa catctaagta cctgaaggaa
      301 gagaaagaaa ttcgattccg taagtagcgg cgagcgaacg cggattagcc caaaccaata
      361 aagttttctt tattggggtt gcggacatat cataacgaag aggtatcgta attgaagagg
      421 tttggaaaga cccaccacag aaggtaatag tcctgtatgt taaacgagaa gacttcagat
      481 atgatccaga gtaccacggg acacgtgaaa ccctgtggga agcaggaggg accaccctcc
      541 aaggctaaat actacctagt gaccgatagc gtatagtacc gtgagggaaa ggtgaaaaga
      601 accccgggag gggagtgaaa tagaacctga aaccctgcac ttacaagctg tggaagcaca
      661 tttcttgtgt gaccgcgtac tttttgtaga acgggccaac gagttacgtt aagtagcaag
      721 gttaagcact taaggtgcgg agccgtagcg aaagcgagtt ttaactgagc gttcagttac
      781 ttgacgtaga cccgaaaccg ggcgacctac ccatgagcag gatgaagcga aagtaaaatt
      841 tcgtggaggt ccgaacccac gagcgttgaa aagctcgggg atgacttgtg ggtagcggtg
      901 aaattccaat cgagcccgga gatagctggt tctccccgaa atagctttag ggctagcctc
      961 aaggtgagag atacggaggt agagcactga atgtcctagg gggtattgca cctaccgaag
     1021 actatcaaac tccgaatgcc gtcatcttat acttgggagt cagactgtgg gtgataagat
     1081 tcatagtcga aagggcaaca gcccagatcg tcagctaagg tccctaaatg taagttaagt
     1141 ggtaaaggat gtgggattgc acagacaacc aggatgttgg cttagaagca gccactcatt
     1201 caaagagtgc gtaatagctc actggtcgag tgatcctgcg ccgaagattt ccggggctaa
     1261 aacttactac cgaagctacg gcatcagtaa tgatgggtag gggagcttcc catacgggtt
     1321 gaagcatgac cgtaaggaca tgtggacagt atgggagtga gaatgttggc atgagtagcg
     1381 agatgtgggt gagaatccca caggccgtaa acccaaggtt tccaggggaa ggttcgtccg
     1441 ccctgggtta gtcgggacct aagctgaggc cgaaaggcgt aggtgatgga caacaggttg
     1501 atattcctgt actaccgata accgtttgag agaagggatg acacagtagg ataagctaag
     1561 cacactgttg gttatgtgtg cccaagcatt gaggcagtca aagtaggcaa atccgctttg
     1621 ataatgctgg gatgtgatgg ggagcgaaat ttagtagcga agtagctgat ttcacactgt
     1681 caagaaaagt ctctatcgag gttaaaggta cccgtaccgc aaaccgacac aggtgggtga
     1741 ggagagtatc ctaaggccag cgagagaact gttgttaagg aactcggcaa aatgaccccg
     1801 taacttaggg ataaggggtg ccaccatcag gtggccgcag agaataggcc caagcgactg
     1861 tttaccaaaa acataggttt ctgctaagtc gcaagacgat gtataggagc tgacgcctgc
     1921 ccggtgctgg aaggttaagg ggatctgtta gagcaatcga agcagtgaac ttaagcccca
     1981 gtaaacggcg gccgtaacta taacggtcct aaggtagcga aattccttgt cgggtaagtt
     2041 ccgacccgca cgaaaggcgt aacgatttgg gcactgtctc aacaacagac tcggtgaaat
     2101 tgtaattccg gtgaagatgc cggatacctg cgacaggacg gaaagacccc atggagctt
//
LOCUS       DAMJVX010000300         2133 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.307, whole genome shotgun sequence.
ACCESSION   DAMJVX010000300 DAMJVX010000000
VERSION     DAMJVX010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.307"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(131..556)
                     /locus_tag="PC407_003606"
     CDS             complement(131..556)
                     /locus_tag="PC407_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2499009.1"
                     /translation="MHDELKIKMYSFTMDCKDPHELAKFYAALLNWEIAFYNEEYACV
                     GAPGTNQGTYPGITFQRNSEYKPPVWPEKPESQQQMAHMDFAVNDLEEAVQYEIHCGA
                     TIAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            complement(709..1170)
                     /locus_tag="PC407_003607"
     CDS             complement(709..1170)
                     /locus_tag="PC407_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF2499010.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            complement(1208..2041)
                     /locus_tag="PC407_003608"
     CDS             complement(1208..2041)
                     /locus_tag="PC407_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2499011.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCMQLKNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMYICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
BASE COUNT          733 a          325 c          250 g          825 t
ORIGIN      
        1 attttttaaa aattatcatt attgataaag ctctactatt tatatttttt agtgtattca
       61 tgtataataa gtaaaagcac aacggtatat actgttgtgc ttttttgtaa ttaataaaca
      121 atattttggt ctataacaat gaaaaatggc tactttccat aattgatttc atctgacata
      181 agcaaaaagg gtgtccagct gggtcaatca taactctcca gtcatcagta aattgctcct
      241 ctgcgattgt agctccacaa tgaatttcat attgaactgc ttcttctaaa tcattaacag
      301 cgaaatccat atgagccatt tgctgttgag attcaggttt ttctggccac acaggcggtt
      361 tatactcaga atttcgttga aatgttatac caggatatgt tccctgattt gttcctggag
      421 caccaacaca tgcatattct tcattataaa atgctatttc ccaattgagt aacgctgcat
      481 aaaattttgc taattcatgt gggtctttgc aatccatcgt aaatgagtac attttgattt
      541 tcagttcatc atgcataacg taattacctc caagtataaa tttcttcgtc ttttcatttt
      601 taagctaaat tcaaacatta agtatgtaat ttatttcaag tatttttcta ttatgcccca
      661 agcctttgtt ttttgcaata taaagaaaaa attatatatt ttaatatttt ataattgttg
      721 aaattgtgta ataaattcaa ctggatacat agtgcgtttt gaaatttctt ctaaagctaa
      781 accttgtttt aaaagcctct ttatgacgct ttccatactt tctgaaattt ctataatatt
      841 tttatcaaaa tcataaaacc taaaagttct ttgaccccat gggtattctt ttatataatg
      901 caaaaattca atatttccta ttctatttat cttactttcc cattgtttga tatcattaac
      961 ctcaaaatac aactgaaagt tatttgattg ttgatatatt ggtaaatcat atccaattat
     1021 agctttataa ttactttgta atccaaatcc attttcaaat gatacatgtt ctcctaaatc
     1081 taatgtaatt ttttgctcca taacatcttc ataaaacttt tttgatttat ttatgtcttt
     1141 gacaattagc aatactcctc caaatttcat cttatcacta tcctttcttt gttataagta
     1201 ttataactta aaattttgca atttcactgt aaaaatcaga catattagct cggtattttt
     1261 gaggagttat tccacagaca attttaaaat ctttaataaa atgtggtata tcatagaatc
     1321 ctaattcatt acatatatac atcaatgaag ttttattatt gtttagaagt tttatagatt
     1381 tattaatacg taccaaacga gaaaatgact tcatactcaa ccctaggtac tgattaaaga
     1441 gtctgtttaa atgcctagaa ctataaaata cttttttaga aatttcttgt gaagatatgt
     1501 ttccagagtt ttgaataatc aactgaattg ccaaaataaa ctcatatgag tattcaattt
     1561 taatattaga aatcaataat tcttccaact ttaaaatcaa tttatctata gataaagatt
     1621 cattaaatat ttttttaatt tctaaatcta atgaagtatt aattatagaa aatggaacaa
     1681 ctttatcaat aagttcactt tgctgtatac ctataagcgg gaaaaaacca gcaggttgaa
     1741 attctataat tagtataaca tcacatctat tagcaatatt tcctactgtt tttggtttag
     1801 tagttggacc aaataataga ctatgtaacc ctgtggcatc ataaaaaaac accaaagtta
     1861 cacttccatg aggaataatc gtgtaattat cagaaattat tgtttgattt ggaaacgata
     1921 tagtaaaatt cgatatccaa ttttttaatt gcatacaagg caagagataa acaaaattgt
     1981 cttcacaaac tataagtgac ctatttttat ttataaaatg aatactttct ttttttaaca
     2041 tatattatat cctgtcctat cgtgaatatt tattaaagta agaagaatcc atattatctc
     2101 tatatatcat taactgcttt taataataga gat
//
LOCUS       DAMJVX010000301         2130 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.308, whole genome shotgun sequence.
ACCESSION   DAMJVX010000301 DAMJVX010000000
VERSION     DAMJVX010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.308"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            92..2116
                     /locus_tag="PC407_003609"
     CDS             92..2116
                     /locus_tag="PC407_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2499012.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGEVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKRAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
BASE COUNT          906 a          249 c          301 g          674 t
ORIGIN      
        1 ataaatttat actactattt gttgtataat ataacttacc ttatacttta aatactttaa
       61 aaataaatac tattttgtta ggagaaataa aatggataaa aagaagttaa tccttataac
      121 atatgataaa ttaaatagtg accactacaa agaagaatta acaaactttt ttggagacga
      181 aataatcata gaaacacaaa atatacttga tggaataaaa gaaaatttag agggagaggt
      241 agtgctttct ctatcacctt taactagtaa ttttttaata aaacatttta aggaagatat
      301 tgaaattatt catggaacga aagcactttc aaaacttgga tatgaaaaaa tgatgaaact
      361 acctccaggt acaaaatcgt tacttatgac aaccaataaa acttcagcat ttgagatggc
      421 tacttatcta tataaaattg gtataaatca tatagatttt gtaccgacat atccagattg
      481 tgatgaaata tatgatttag atacagctat aacaccaggt cagattagat ttataccaaa
      541 atacataaaa aatattgttg atttaggttg gagaaaaatc tcattagata catatatgtc
      601 attattagta gtattaaaac taaaaaatga gaaatttata gaaaaactat acaaattgtc
      661 caaggagaca ctaagtcatg atttcttaaa tacgtcatta gataatatat ctaaattaaa
      721 aactatactt tacatgacca ttgatgaaat aggtgatggt ctaatatttt tcaacacctt
      781 taacaaggtt acttttgtaa ataagtcttt acttaatatg ttggaactag atgaaaaact
      841 tataaaatca ccttccttaa tggaatatat gccaaagagt ttcttagata aaattacaaa
      901 gaacttaaat atcgataata tgattatata catagacgaa atagataaaa aattcatact
      961 ttctaaaaaa ccattttatc tttataaaaa tatagagggt tgcttaatta cattaaaaga
     1021 tgtaaacaat atagaaatat tagaacaaaa aataagaagt gattctgtca aaagaggtta
     1081 tgtagctaaa tataaattta ataatattat tggaaatagt tctattataa aagattgtat
     1141 taaaagggca aaaaaaatgg ctcttacaga taatcctata ttaataacag gtgaaacagg
     1201 aacaggtaaa gaagccttta ctcagtcaat acacaatcat tcaaatagaa aaaataaacc
     1261 ttttgtagca ataaattgtg cttcattacc aagtgaatta cttgaaagtg aactatttgg
     1321 atatgaagat ggttctttta ctggagcaaa aaaaggtgga aaaaaaggat tattcgaatt
     1381 agctcatact ggcactatat ttttagatga gataggagat atgccacatg atttacaggt
     1441 taaactttta agagtcttac aagaaaaaga aattagaaaa attggtggta caagtattat
     1501 tccaattgat gtgagaatat tagctgccac aaataaagat ttagaaaaat taattataga
     1561 aaataaattt aggatggatt tattttatag gataagtatg ttcactttag atttacctcc
     1621 tcttagaaag agattagaag atatacctct tcttttagaa tcatttttaa aagaacttcc
     1681 ctataaaaac ataaaactcg ataagagtct tttagaagct ttaaattcat acacttggat
     1741 ggggaatatt agagaactga gaaattgtgt tgaatatatg gcttacatgg gttccaatta
     1801 tcttacaata aatgatttac ctcaaaacat atcttcaaaa ttaaataata atcatatgtc
     1861 aagtaatatg tctatattca atgatttaaa ccaatatgac aaaaacatat gcatttctat
     1921 cctaaaatca ttgcacatga aaccaatggg gaggacaaag ttaatgaaat ttatggaata
     1981 taatgtcaca gaatacgaag taagaaatat gctagaatat ttgacacgca atggatactt
     2041 aatttctagt aaaggaagaa aaggctcatc aataactgag aaaggcataa aaataataga
     2101 aaataatata atttaaacct atagtaattc
//
LOCUS       DAMJVX010000302         2062 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.309, whole genome shotgun sequence.
ACCESSION   DAMJVX010000302 DAMJVX010000000
VERSION     DAMJVX010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.309"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            325..771
                     /locus_tag="PC407_003610"
     CDS             325..771
                     /locus_tag="PC407_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDF2499013.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            792..1835
                     /locus_tag="PC407_003611"
     CDS             792..1835
                     /locus_tag="PC407_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDF2499014.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT          848 a          217 c          384 g          613 t
ORIGIN      
        1 taaaagaagg attaagacta ataacgattc aaaataaata acttatgtaa gaaccgtagg
       61 aactacgggg atagcctgtg gagaattagt aagacatatg ttagtatgca aaattctatg
      121 aagcaggaac cctgtgactt tagtcatggg aggttcagac tactttgtaa caattttaac
      181 aaagtatgtt gatataatac tattaaatta atcttagatt agtttattct attgatttgt
      241 agtttaaatt tatattcaaa aacattgtat aaacatataa tttatatagg gttttataat
      301 tcatatgaaa agagggtaga tagtatgaag ttaatatggt tgatagtagc agtcttattt
      361 ggaatagcag aaatgttaac accaagctta acattgatat ggtttagtgt aggagctgta
      421 atacttatat ttttaagtag ctttatagag agtattttct tacaaatttt aatatttgca
      481 gtaatttcta tagcaatgtt agtcgtagct actaaaaaaa tagtaaaaaa agataaaggc
      541 tataaatcta acactaattt acaagcaatg atgtctaaaa aaggtattgt tacagaagag
      601 atttctccaa ataacacagg tctagttgta gttgaacatg aaacatggac ggctatttct
      661 atagatggtg aaaagataga aaaaggttct acagtagaag tattaaaaat tgaaggggta
      721 aaattagttg ttaaaaaagt agacgccaca gttaatgtga caaaccaata gaataaagga
      781 ggatttatag tatgggtact aaaattgttt taagtattgt attaatagta gtagtcgtag
      841 caataagttt aacgtgtata agagttataa aacaatctaa agtaggtata ataatgagac
      901 ttggtaagtt tcaaaaagtg gctgaaacag gtgtacattt cttaatacca tttttagata
      961 aaatggcata tgtaattgac cttagagaaa ttgtaataga ttttccacct caaccagtta
     1021 taactaaaga taatgtaact atgcagattg atacagttgt atattataaa gttacagatc
     1081 cagttagata tgtgtttgag atagctaatc caatagctgc tattgaaaat ttaacagcta
     1141 caacactaag aaatataatt ggtgaacttg atttagatga aacattaaca tcaagagata
     1201 taataaatgt aaaaatgaga actatccttg atgaagcaac agataaatgg ggtataaaag
     1261 taaatagagt agagttaaaa aatataatgc ctcctcagga tattcaagtt gcgatggaaa
     1321 aacaaatgag agcagaaaga gaaagaagag aggcaatact tcaagcagaa ggtaataagt
     1381 cagctgcaat acttcaagca gaaggtgaaa aacaatctgc gatattaaca gcagaagcca
     1441 aaaaagaagc tatggtacgt gtagcagaag gtgaaaaaga atctgctata ttagtggcag
     1501 aaggtgaagc tgaagctata agacagaccg ctattgctaa ggcacaaggt gaggctgaaa
     1561 tgataaaaag aactcaaatg gcaacagcag aaggtttaaa attagttttt tcagcaatga
     1621 aagaggctga tatagacaat aatattctag cattaaaatc tatggaagct cttgaaaaaa
     1681 tggctgaagg taagtcaaca aaactggttt taccttcaga agcagttaat ttcctaggaa
     1741 cattcaaagg aataaaggaa gttatgagtg atgataacaa agaagtactt gatataaaag
     1801 aagttttaaa tgataatgaa tcattaaaaa aataaaagag ttttgttatc ataggaagga
     1861 tatggaaatt aaaggtcgaa ttaattaaat atagcacaaa aatgcacgtt gaaaagaaga
     1921 ggacttataa atttttaagt cttcttcttt gtttttatta gaaagatgaa attataaaaa
     1981 tgaattaaca gaatctttat acaatttatc ctttactaat gtaaaaagtg ttatataata
     2041 ttatataaaa catatagcaa at
//
LOCUS       DAMJVX010000303         2054 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.310, whole genome shotgun sequence.
ACCESSION   DAMJVX010000303 DAMJVX010000000
VERSION     DAMJVX010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.310"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            128..1441
                     /locus_tag="PC407_003612"
     CDS             128..1441
                     /locus_tag="PC407_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HDF2499015.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEENIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            1459..1995
                     /locus_tag="PC407_003613"
     CDS             1459..1995
                     /locus_tag="PC407_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDF2499016.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENKDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
BASE COUNT          798 a          199 c          404 g          653 t
ORIGIN      
        1 attatatgtg tttagttgga tttgtgtatt taaataatgt ataatttatg ctgattactg
       61 ctaaaaaagc ataataatat tgtattgtaa ggttttataa acttctaatt gaaaggggat
      121 aattcaaatg gataagagat atttagaatc cgatgtattt gaagcttttc aagaaagact
      181 ttcttatctt tttaatgaat ttgattatat ctatgtatct tttagtggag gaaaagatag
      241 tggcttactg ctaaatttag ttttggatta taagagaaag aataatataa gttccaaaat
      301 aggagtattt catcaagatt ttgaggctca atatagtttg actactgaat atgttgaaaa
      361 agtatttgaa gagaatattg aagatattga accatattgg gtgtgtttac caatggctac
      421 aagaactgct gtaggaaatt atgaaatgta ttggtatcca tgggatgatg agaaagaaga
      481 attatgggta agagaaatgc cagtcaaaga atacataata aatcaaaaaa ataatccttt
      541 tacttattat aagtataaaa tgagacaaga aagtttggca aagcaatttg gtagatggat
      601 tagagatata catgaaggtg caaaagttgt ttgcttatta ggaacacgag cagcagaatc
      661 tctacaacgt tattgttcta ttgtgaataa acagcatgga tataaaggaa agtgctggat
      721 aactaaggaa tttaaaaatg tctggacggc atctcctata tatgattggg aaatagaaga
      781 tatatggtta gcccactata aatttgatta tgactataat aaaatttatg acttattcta
      841 taaagctgga gtagctccaa gaaaaatgag agtggcatca ccttttaatg attcagcaaa
      901 agagagttta aatttataca gaatattgga cccagatgta tgggtgaaat tggttgctag
      961 agtacaagga gttaatttta cttctattta tggaaaaaca aaagctatgg gatatcgtag
     1021 tgttaaattg ccatatggac acacatggaa aagtttcaca tattttttgc ttgatacctt
     1081 acctgaaaat ataaggaata gctatattaa aaaattttca acatctattg agttttggca
     1141 gaatacaggt ggtggtttgt cagaaatagt tatacgtgaa ttgttgagaa aaggttataa
     1201 aatagaatta aatggagttt caaatcatac aaaagataaa aagagtagag tcatattttt
     1261 ggatgatata cctgatgata cggatgatat taaatttacc aaagatatac caagttggaa
     1321 aagaatgtgt tattgtattt taaaaaatga ccatatgtgt agatttatgg gatttggtcc
     1381 tacaaaagaa cagagaatta gaacggaata tattaagaag aaatatgaaa aaattcatta
     1441 gataggagtt aagatgctat ggaatataaa agtcctgtat atggaatcat cgcagtgcca
     1501 atcgaaaaga tagaatctaa cgactataat ccaaatgcag tagccccacc agaaatgcag
     1561 ttgttgtata aaagtataaa agaagatggg tatacaatgc caattgtctg ttattataat
     1621 gaggaaaagg ataagtatat tattgtagat ggttttcata gatatagagt aatgcttgaa
     1681 aataaggata tatatgatag agaaaacgga cttcttcctg tttcagtaat tgataaatct
     1741 cttgatgaaa gaatggcaag tactattcga cacaatcgtg caagaggttc tcataatgtg
     1801 gatttaatga gtaaaattat aactgaactt tgtgaaattg gaaaatctga tgaatggata
     1861 tcaaagcacc tgggtatgga agttgatgaa attcttagat tgaagcaaat aactggatta
     1921 gcttatttat ttaaagataa agagtttggt aatgcatgga ttccctttgg ctctgatata
     1981 gaagaagaaa attaaagaga tttattaaaa gaaaaagata tattaaaaag agaaaattta
     2041 aaatatatat tgaa
//
LOCUS       DAMJVX010000304         2028 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.311, whole genome shotgun sequence.
ACCESSION   DAMJVX010000304 DAMJVX010000000
VERSION     DAMJVX010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.311"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(111..650)
                     /gene="scpB"
                     /locus_tag="PC407_003614"
     CDS             complement(111..650)
                     /gene="scpB"
                     /locus_tag="PC407_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDF2499017.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            complement(652..1371)
                     /locus_tag="PC407_003615"
     CDS             complement(652..1371)
                     /locus_tag="PC407_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDF2499018.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            complement(1401..1997)
                     /locus_tag="PC407_003616"
     CDS             complement(1401..1997)
                     /locus_tag="PC407_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HDF2499019.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLIYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGIYQIILSPIYTVLLKIVYLFV"
BASE COUNT          640 a          335 c          186 g          867 t
ORIGIN      
        1 ttttaagaga attataattt tatatttata caaaaaaaca caacaactta gagcatttta
       61 aatcttttaa aatttaaaat tacatactaa attattgtga ttttatattt ttattcattt
      121 gttgccactt cttgataatt ttctataggc ggcaattctt caagactttc tatattcaac
      181 aactttaaaa attcatctgt tgttttataa attattggtt tccctatctt attcaatcta
      241 ccagcttctc ttataagttc attttcaaac aaagtttgta ataccttatc acattttact
      301 cctcttatat cctctatttc tacctttgtt attggttgtt tgtaggcaat tatagtaaga
      361 gtttctaatg tagcttggct caaacttttc tttttctttg gttcaataat cttctttata
      421 tattctgcat agtctttatt tgtacacatt tggtacttat tctctagctt gattatttga
      481 atacctctat tttgttctct gtactcctca ataagtaaat ttaacataat ctctatttct
      541 tttgatgaaa gttctttatt tattatatag tttaattctt ttatacttat tggttcccca
      601 tatgcaaaca taacagattc tataatatac tttatatctt ctcttttcat tttaacactc
      661 cgaacttttt cttattagaa tatcatcaaa aaatatgtct tgtactacta ctatttcttt
      721 ttcttttata agctctaata tagataaaaa agtagctatt atttcatcat tttcatatga
      781 ttttataata tttgtaaaag taacttcaat cttatctttt ataatatctc ttatatacaa
      841 tatcttttct tccacagata ttatcttctt ccttactatt ttgtctagtt tctcatcatt
      901 agcattttct atctgtgatg ttttaacttt aaatatgtat ggaagtatat tttttattgc
      961 ttcaattgat atatcttcta aatccacatt gtcatcaata attatctctt ctttatttct
     1021 ataaaatctc tcattaacat aagttatatt ttcttttata tcttgtgatg ctaccttaaa
     1081 ctttctatat tcttctaact tttccataag ttctattctt gggtcttctt cttcatcttt
     1141 ttgcttataa agcagatatt tagactttat ctctaaaagt tttgatgcca tagttataaa
     1201 ttcactagtt atctctaaat ccattttatc tagcattttc aaataattta gatactgctt
     1261 agttatatct attatagata tatcctttat atctatttta tgctttgtta ttaaatcata
     1321 aagtaaatct aatggtccct catagacttg taattgtata ttatacttca tatcaatccc
     1381 tctttaatct gtttattata ttaaacaaat aaatatacta tttttagcaa cactgtatat
     1441 attggtgata gtattatttg atatatacca gtaaataaag caattaataa aattatataa
     1501 ctcatacttt caaatttata caaatattca ttccatttat atggtacaaa tgtagatata
     1561 actccccatc catcaagtgg aggtatcgga agtaagttaa aagctgcaaa tcccatacta
     1621 tatataagca catatccagc tattgtattt atagcataca tatgcacata cttattgata
     1681 attacacaca gtatagcagt caaaacatta caaaaaacgc ctgcaagtga aactatcagg
     1741 tttcctatct tatagttttt aaaattattt ggatttactg gtactggctt tgcccaccct
     1801 atcttaagga taaacatagc caaaagacct attggatcaa catgtcttac aggatttaat
     1861 gtcattctac cataagcttt agctgtatca tctcccaata agtgagctga ataagcatgg
     1921 ccaaactcat gtattgatat tgccatagct ataccaacta aacttgctac tatttctcct
     1981 atattcatac ttaacattaa tgtacatctc ctaaattaaa tttttaat
//
LOCUS       DAMJVX010000305         1987 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.312, whole genome shotgun sequence.
ACCESSION   DAMJVX010000305 DAMJVX010000000
VERSION     DAMJVX010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.312"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            54..623
                     /locus_tag="PC407_003617"
     CDS             54..623
                     /locus_tag="PC407_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499020.1"
                     /translation="MKLRNPKIKKLLSVALLAALIGIPIGQTANAMEISNNVPSYIEN
                     NPTTIEDLEINDSQLPKPGTVTYIDINKLIKNLKENGIDTTLIENELYYRKAGTNKIV
                     WTKTGFDLYLSKGVALTLAGAGTGVAGLLAGAIPGIGQYLAAAIVGAITMHLGSNITS
                     GKIFKFKKMRYNKGGAYVTKYYLQSVRNQ"
     gene            915..1232
                     /locus_tag="PC407_003618"
     CDS             915..1232
                     /locus_tag="PC407_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499021.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYISIGLFTRRLDITIIAIILVATITVILYYLVRKRY
                     ITI"
BASE COUNT          816 a          227 c          269 g          675 t
ORIGIN      
        1 acaataataa tatatatctt tgatatataa ctaatttaaa atggaggtat tttatgaaac
       61 taagaaatcc aaagattaag aaattgttat cagtagcatt attagcagct cttataggta
      121 tacctattgg acaaacagcc aatgcaatgg aaatttcaaa taacgtccca agttatatag
      181 aaaacaatcc aacaacaata gaagacttgg aaatcaatga ctctcaatta cctaaaccag
      241 gtacagttac atacatagat attaataagc taattaaaaa ccttaaggaa aatggtatcg
      301 acactactct tattgaaaat gaattatatt atagaaaagc tggaacaaat aaaatagttt
      361 ggactaagac aggctttgat ttatatcttt ctaaaggagt tgcattaact ttagctggtg
      421 ctggtacagg ggttgcaggg cttttggctg gtgccattcc tggtatagga cagtatcttg
      481 cagctgctat agttggagca attacaatgc atcttggttc aaatataaca tctggaaaaa
      541 tctttaaatt taaaaaaatg cgttataata aaggtggggc ttatgtaacc aaatattact
      601 tacaatctgt aagaaatcaa taggtgataa aatgattata aaatatctac tttttgcaat
      661 tatctcagca ctagttttat tcttttgtaa taaagtattt caactaagta aattacacaa
      721 gttcattata ttgtttgttt cattttttat actaactttt gtagcatcat ttattaatag
      781 ataaaaaata agatggtggg gttaacctac catctttttc tcatttaata tttgtaatat
      841 tttagatact ctttatactt gtgttactga tatactttga ttaagaaaag taaatttaac
      901 aggaggtatc tgctatggat tttggaaata tcaatcttat acttattgga ataatagtta
      961 taataggaac tacaataata tatttaatta aacctaaaac tgctttttgt agtaaaaaat
     1021 attttaataa attagaatca atatatggaa atatagataa gaaaaaaact gttaaactag
     1081 aagtacttta tcgctatgta acaggtcttg agtatatatc aataggctta tttacaagaa
     1141 gattagatat tacaataata gcaataatat tagtagctac tataacagta atattatatt
     1201 acttagttag aaagagatat ataactatat aaaatctaat agaattaata tcaaaagaaa
     1261 gtagcaacta atcaccagta atgtaaaata atttgttttt caaagttaat aaaagtcttc
     1321 tttatggcaa tgatttaaat ataatcaaat atcgtaacgg agattttttt atgaataatt
     1381 taagaaaaca attaatcaaa gaaattatat aagatggaga acttataaaa tctacagata
     1441 taagtagttt taggagtaaa tacagatgac ttatttcaaa gagacaatag ctacaagtta
     1501 tcctcattct gtaatacaaa aatgtttaat acattagata agaaatactt taaatatgtt
     1561 tcatacaagg actcaaaaga attaataagt gattttaaat ctgtatatag gtcagttaat
     1621 gaataaaaag ccttatcgac tttagataaa atagattaaa aataatgtaa acagtatact
     1681 tcagaaatta aaacgtttgg aaataactgg aatgttgtag catcagcctt tagttttact
     1741 aaaataagta atatctataa tatatattac aagtataatc gaaggattta ataaatattt
     1801 caaaaatgtc tcaaaaacaa agtccagatt tacatcaaac gcttcactct taaagatatt
     1861 attcttgaca agcgaaaatg tgatgaaaaa atggactatg aaatataaaa attgatataa
     1921 tatatacaat catttattca tatcttttga tagtagaata taattttaaa aattataccc
     1981 taaaaat
//
LOCUS       DAMJVX010000306         1983 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.313, whole genome shotgun sequence.
ACCESSION   DAMJVX010000306 DAMJVX010000000
VERSION     DAMJVX010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.313"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            178..294
                     /gene="rrf"
                     /locus_tag="PC407_003619"
     rRNA            178..294
                     /gene="rrf"
                     /locus_tag="PC407_003619"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            301..375
                     /locus_tag="PC407_003620"
     tRNA            301..375
                     /locus_tag="PC407_003620"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:333..335,aa:Asn,seq:gtt)
     gene            380..454
                     /locus_tag="PC407_003621"
     tRNA            380..454
                     /locus_tag="PC407_003621"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:413..415,aa:Glu,seq:ttc)
     gene            460..535
                     /locus_tag="PC407_003622"
     tRNA            460..535
                     /locus_tag="PC407_003622"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:493..495,aa:Val,seq:tac)
     gene            541..617
                     /locus_tag="PC407_003623"
     tRNA            541..617
                     /locus_tag="PC407_003623"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:575..577,aa:Asp,seq:gtc)
     gene            628..702
                     /locus_tag="PC407_003624"
     tRNA            628..702
                     /locus_tag="PC407_003624"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:660..662,aa:Thr,seq:tgt)
     gene            717..801
                     /locus_tag="PC407_003625"
     tRNA            717..801
                     /locus_tag="PC407_003625"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:751..753,aa:Tyr,seq:gta)
     gene            811..884
                     /locus_tag="PC407_003626"
     tRNA            811..884
                     /locus_tag="PC407_003626"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:843..845,aa:Gly,seq:tcc)
     gene            895..971
                     /locus_tag="PC407_003627"
     tRNA            895..971
                     /locus_tag="PC407_003627"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:929..931,aa:Arg,seq:tct)
     gene            981..1056
                     /locus_tag="PC407_003628"
     tRNA            981..1056
                     /locus_tag="PC407_003628"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1014..1016,aa:Gln,seq:ttg)
     gene            1068..1143
                     /locus_tag="PC407_003629"
     tRNA            1068..1143
                     /locus_tag="PC407_003629"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1101..1103,aa:Lys,seq:ttt)
     gene            1146..1234
                     /locus_tag="PC407_003630"
     tRNA            1146..1234
                     /locus_tag="PC407_003630"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1180..1182,aa:Ser,seq:tga)
     gene            1252..1342
                     /locus_tag="PC407_003631"
     tRNA            1252..1342
                     /locus_tag="PC407_003631"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1286..1288,aa:Ser,seq:gct)
     gene            1351..1427
                     /locus_tag="PC407_003632"
     tRNA            1351..1427
                     /locus_tag="PC407_003632"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1385..1387,aa:Pro,seq:tgg)
     gene            1513..1588
                     /locus_tag="PC407_003633"
     tRNA            1513..1588
                     /locus_tag="PC407_003633"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1546..1548,aa:Trp,seq:cca)
     gene            1649..1725
                     /locus_tag="PC407_003634"
     tRNA            1649..1725
                     /locus_tag="PC407_003634"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1683..1685,aa:Pro,seq:tgg)
     gene            1732..1808
                     /locus_tag="PC407_003635"
     tRNA            1732..1808
                     /locus_tag="PC407_003635"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1766..1768,aa:Ile,seq:gat)
     gene            1812..1887
                     /locus_tag="PC407_003636"
     tRNA            1812..1887
                     /locus_tag="PC407_003636"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1845..1847,aa:Phe,seq:gaa)
     gene            1895..1971
                     /locus_tag="PC407_003637"
     tRNA            1895..1971
                     /locus_tag="PC407_003637"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1929..1931,aa:Met,seq:cat)
BASE COUNT          501 a          449 c          535 g          498 t
ORIGIN      
        1 aatgtattta gcttatcgat actaataggt cgaggacttg accaatatta tttgatgtta
       61 ttcattaaga tatatgtgca gtttttagag tgttaactct aaaaaaaaat aaaaaaagta
      121 ttgacaaaaa tagtaaaaaa tgttaatatt atacttgtcc tagcaacaag aaaaaaatgt
      181 ggttataata gcagagaaga tacacctgtt tccattccga acacagaagt taagttctct
      241 agcgctgatg gtacttggtg ggaaactgcc tgggagagta gggcatagcc acgtgaatgt
      301 tccaaggtag ctcaacggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc
      361 ccacccttgg agccattatg gctcgttggt caagaggtta agacaccgcc ctttcacggc
      421 ggtaacaggg gttcaattcc cctacgagtc accatttgtg ggatcatagc tcagctggga
      481 gagcacctgc cttacaagca gggggtcaca ggttcgagcc ctgttggtcc caccatttgc
      541 ggcctggtag ttcagttggt tagaatgcca gcctgtcacg ctggaggtcg agggttcgag
      601 ccccttccag gtcgccaaaa taaaaatgct ggtgtggctc aacggtagag cagctgactt
      661 gtaatcagca ggttgtaggt tcgattccta tcaccagctc catgtaaaac taatacggag
      721 gatttcccga gcggccaaag ggggcagact gtaaatctgt tgtcatcgac ttcggtggtt
      781 cgaatccacc atcctccacc aattaaatat gcgggtgtag ctcaatggta gagttctggc
      841 cttccaagcc agctgtgagg gttcgatccc cttcacccgc tccaaagaaa gcgtgggtgc
      901 atagctcagc tggatagagc aacgcccttc taaggcgtgt gtccggggtt cgaatccctg
      961 tgcgctcacc acatatatat tggggattag ccaagtcggt aaggcacatg actttgactc
     1021 atgtatgcgt aggttcgagt cctgcatccc cagccaaata aaaatatggt ctattagctc
     1081 agtcggtaga gcacctgact tttaatcagg gtgtcccgcg ttcgagtcgc ggatagatca
     1141 ccaatggaga ggtgtccgag tggtttaagg agctggtctt gaaaaccagt gacttcgaaa
     1201 ggggccgtgg gttcgaatcc caccctctcc gccaaaaaaa tgactgaatt tggagaagta
     1261 ctcaagtggc tcaagaggat cccctgctaa gggattaggc tgggtaaccg gtgcgagggt
     1321 tcgaatccct ccttctccgc cattcaaaaa cgaggtgtag cgcagtttgg tagcgcacat
     1381 ggtttgggac catggggccg ggggttcgag tcccttcacc tcgaccaact taagctttta
     1441 gttcagttgg ttagagtgcc caactcatgg ctagtaattc tagggtttga gtccatgaag
     1501 gttattcttc ataggggtgt agctcagttg gtagagcgtc ggtctccaaa accgtgcgcc
     1561 gggggttcga gtccctccac ccctgccaaa taaaaaggac ttgaaaaaag ttggatttta
     1621 gagtagtata agatagtaaa taagatttcg aggtgtagcg cagtttggta gcgcacatgg
     1681 tttgggacca tggggccggg ggttcgagtc ccttcacctc gaccaagaaa agggcctata
     1741 gctcaggtgg ttagagcgca cgcctgataa gcgtgaggtc gctggttcga gtccagctag
     1801 gcccaccatt tgcccagata gctcagtcgg tagagcaggg gactgaaaat ccccgtgtcg
     1861 gtggttcgat tccgcctctg ggcaccagga atacggcggt atagcttagt tggctagagc
     1921 gttcggttca tacccgaaag gtcataggtt cgactcctat taccgctacc atctaactat
     1981 atc
//
LOCUS       DAMJVX010000307         1980 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.314, whole genome shotgun sequence.
ACCESSION   DAMJVX010000307 DAMJVX010000000
VERSION     DAMJVX010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.314"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(44..1261)
                     /locus_tag="PC407_003638"
     CDS             complement(44..1261)
                     /locus_tag="PC407_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HDF2499022.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(1452..1865)
                     /locus_tag="PC407_003639"
     CDS             complement(1452..1865)
                     /locus_tag="PC407_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2499023.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKTEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT          678 a          325 c          223 g          754 t
ORIGIN      
        1 attttttaga ttatatattt taatatactt tcttatataa aaattatttt aaatcaagat
       61 tatctagtat ttttaaaaat atttttacag cagtatccaa gtcatctaca gaaacacttt
      121 cattaggatt atgacttacg cccccagcac atcttataaa taccatacca atatcagtta
      181 tattatccat ttcctgagca tcatggccag caccactata aatataaaaa ggattaagat
      241 ttaaatctat aaaacttttt tctatgattt ttgttatttt attagaacat ggtacaggaa
      301 cattttcaaa agcaagttca cttgtataat taagttttct attttcacat atatgtgata
      361 tttcattaaa tatcttttct acagaattat caagaatttc ttgtgaacaa gagcgtacat
      421 cgagtgtaaa ttcagctctt tcaggtacca catttgatga agatggatag aaattcatct
      481 ttcctacagt agcaaccaaa tcagcagtcg ttttagctac tttttcgata gctaatacac
      541 attcacaggc acaacatcca gcatcaagtc tcatattcat tggaagtgta ccagcatgtc
      601 cagacttacc atttactgat atcttaaaac ttttaaatcc tgtaatactt gatacaattc
      661 cagcaggaag agattcattt tctaaaacag gaccctgctc gatatgaaat tctaaatatg
      721 catctacatc ttttctagga tgcaaatttg ttaagttttt tgtattaaat ccaaactttg
      781 ttacagcttc ttcaagtgta attccatttt catcaacaga tttaagaagt tcctctttaa
      841 aagtaccagc aaaaacttta cttcccatta aagaagtttc aaatctagtt ccttcctctt
      901 cagcgaaagc aattagctta atattgaaag gataagacct attattgtgt tttgcttctt
      961 caagaccaac tagaggcact attataccta acataccatc aaatatacct ccacacttta
     1021 cactatcttg atgagatgca actactaatg tgggagcatt tggaaagctt gatttatatg
     1081 tacctataac gtttccaatg tcatcaattt caatatcaag atttaaacct tccatccatg
     1141 aactaattaa atcaatagat tttttatgtt cttgagtcaa gataagtctt gtaagaccat
     1201 ttgttgttga acttattttt cctaatgttt gtagataatc catacatttt tttcctaaca
     1261 tatttacctc ctaaaaatat aatttttatt taagctaaga ttagctttta tacttataat
     1321 ttttgaactt tcaaataatt ttgtatgtat agtaaaatac tattttagta ttaataatga
     1381 tataaaaaca taaagtctgt agacgaagag cagaagtttt aagttaaaaa tataaaatat
     1441 gatttttatt attatagtat ttttctataa taatatctct ttccagttag aggataattt
     1501 tcaagtgaaa atactttatg gtatccaaat ttctcataaa atttaggtgc ttgaaaactg
     1561 aatgtatcta aaaaaacata tttacaacct cgttctttag caattttttc agtcttatat
     1621 aaaagttgac ttccagtacc tttatatcta agcttctcac taacccataa gtattttata
     1681 gaaagccaat taccatgagt aacacctata aaacctgcaa caattttacc tgctttatct
     1741 cgaatatata ttcctaaatc cactgggttt ttatcttcaa gtctagcaag attatatttt
     1801 aataatcctt gaaaaatagt atcttcatct tgtttttcta ttttttctgt aatttctaat
     1861 tccatatttg aactttctcc ttgtataatc ttttgtatat tggtattttt taataaaatc
     1921 agtagaaaga atatagttta acattattct aataggcatt tatgctaatg tcaaactaat
//
LOCUS       DAMJVX010000308         1971 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.315, whole genome shotgun sequence.
ACCESSION   DAMJVX010000308 DAMJVX010000000
VERSION     DAMJVX010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.315"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            362..655
                     /locus_tag="PC407_003640"
     CDS             362..655
                     /locus_tag="PC407_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2499024.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            636..1277
                     /locus_tag="PC407_003641"
     CDS             636..1277
                     /locus_tag="PC407_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDF2499025.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVILQI
                     LMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKQNYSVGIILPWTLDDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            1521..1853
                     /locus_tag="PC407_003642"
     CDS             1521..1853
                     /locus_tag="PC407_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDF2499026.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
BASE COUNT          754 a          180 c          302 g          735 t
ORIGIN      
        1 tttttataca gtatatttct tatttcattc ttttctattt aaataggact taactacttt
       61 ttaaattgga tttagctact tttaatttga aattaaaagt atatgtaata tatataagta
      121 tatcagtcat taggtttttg ctttaacaat tgtggtgcaa tacacttttg ttaaattaaa
      181 aaaattagat aaaataagat tatataaaag cgacttaagt aaattgagaa tcttaatatg
      241 aaaattttca ataaatattt ggaaataact atttagaaat ttttctaaat agttattgaa
      301 ataaaatgga tgttatgtta tattctattt agatgaaatt ctaaatagcg aggtgatggt
      361 gttggggttt ccagaaacat tcaaagctct ttcagaccct ataagaagag aaatattggt
      421 tatgttgagg gagaataaaa tgtcagcagg agaaattagt gaaaaattta atatgacgaa
      481 tgctacaatt tcttatcatc tatctcaact aaaaaaggca ggactcatat ttgaaacaaa
      541 gtataagaac tttattttct acgaaataaa tatttcagtt ttcgaagagg ttatgctttg
      601 gttttcacaa tttatggaga agggagaaaa agataatgaa aaaagcgata aataaacaat
      661 ttattttatc tactttaata tgttttatac catttattgt atcaatatat ttttataata
      721 gattaccaaa tgaagttgca attcattttg ataattatgg aaatccagat aattatgctc
      781 caaaggttat agcagctttt ggtgtaccat tgttaatgct atgtatccat ttgtatacat
      841 ggtttagact tgagaatgag cctaaaaaag ctaatttttc aaatgttata caaaatttat
      901 caaaatgggg agttgctata ctttcagtaa ttctacaaat actgatggtt ttatattcaa
      961 caggagttag tttggagtct aatttttata caggattaat tttaggaata atagttattt
     1021 tatttggaaa ctatcttcct aaatgtaaac aaaattactc agtaggaata attttgccat
     1081 ggactcttga tgatgaagat aattggaata agacacacca tttagctgga tggatttggc
     1141 ttattggtgg aatattatta attattaatg catttattaa tattcctttt tataatatct
     1201 ttgtaatctt tgtaattgta atactaccat ttatatattc ttatttgatg tataaaattt
     1261 caggtaaaaa taactaatat aattaatcac tataataatg tttggataag aataatatat
     1321 ttcagaactg agatagataa taaagaaatt actaagattt ctattttagt tctgaaaaaa
     1381 tcttacttat atgataactt attgagattt caaaaaataa tataaattag gctaatgaac
     1441 ataaaatgaa aatttgtatt taatattgaa aatataagta cagtgtgata ctcttataag
     1501 aaaaacatag gaggtagaag atgaatacta gagaaatgaa agaatatagg ggattttaca
     1561 ataaaaaatt aagtgaaaca gatgagtttt ttagagtatt tggaagtctt gatgataaga
     1621 cttattcaga aggggctata agtaaaaaaa ataaggaatt aatgggacta gctatttcaa
     1681 ttttaagccg atgtgatgag tgtatttgtt atcatattga gggttgtatt aattctggag
     1741 caagtaaaga tgaaattata gaagctataa aaataggtgt aattggtggc gggtctatta
     1801 catatccaaa cgcaagattt gcaataaaaa ttttagaaga atttaatata taattttgat
     1861 acacgtagtt tatttagact aataataata ttctatttaa gtctttgaaa ttaagcttta
     1921 ttttctaagt tcacttctta ttaagttttt agaattgcat atataaatat a
//
LOCUS       DAMJVX010000309         1950 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.316, whole genome shotgun sequence.
ACCESSION   DAMJVX010000309 DAMJVX010000000
VERSION     DAMJVX010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.316"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            193..768
                     /locus_tag="PC407_003643"
     CDS             193..768
                     /locus_tag="PC407_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499027.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            866..1864
                     /locus_tag="PC407_003644"
     CDS             866..1864
                     /locus_tag="PC407_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HDF2499028.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
BASE COUNT          811 a          169 c          332 g          638 t
ORIGIN      
        1 taaaagatta ataaaaaata tttaacgata aaagctttat tctatatgat tctaagatag
       61 aggatttata agtgtttaag gtgaattaaa tataagacaa cttcttaaag caaatgggtg
      121 tggtatttaa atctaaatat agacatataa tattaaatat gctaatatat caaaataata
      181 aggggggaat ttatgaagct taaagaagca ttgattacat gtgttgtatg tagtgtatgt
      241 ttaactccat tattggtaaa tggagaaaca aaaatagatg cagatcattg taaagaatct
      301 atagtaaata tagatactat agtaaagtca ggtaaaagct ttaattccaa gtataatcaa
      361 ggtatggaag attataaaaa tagtgatgag tataagttga aagtacaaaa ggagaaacta
      421 caaaatcagt taggtattaa aataaagaag ttagtaccta taacttttga agttagttat
      481 tacacaagtc ttaatcaaga aaatggatat ggagcgataa ctgctacagg agatgattta
      541 gaagatggtt ttgtagcaaa caatcacttg agttttggaa ctaagattat tgtagatgga
      601 aaattaaaaa ctgttatgga tagggggtct agtaagtatt ttagtaattc taatgcaata
      661 gatgtatttg tgcccaaatg gagtaatgaa agtgaatcag agtactatga tagagtcaat
      721 aatatgggaa gacattacaa aaaaggctac attattgttg aagaatgatg ttctttgaag
      781 taatatgtat tgattgtagt atttaatata tatataataa atataaaact attagtacaa
      841 tgagatatat tactaggagg gcaatatgga acaatcgttt ataaatggaa aagtaaactt
      901 attaatagaa aattacaaag cattaaatga agttaaaggt tcttggcaaa tgggattgat
      961 acaacatagt tgtgcacttg catttactct taagaacaag agaataagtc ctagattagt
     1021 ggaagaacga atagagctta ttaaaaaaaa tactggatta ttctctaatt ttagaggata
     1081 taatatgttt tatatggcaa cactactttc gtttgaatca aatccagaaa gtagttttaa
     1141 gatgatttta gatatataca aagaacttaa aagtgaaaag ttttggggcg atacatattt
     1201 accacttaca gcatcaatag tttatgaaaa tagagagaaa atggattatc ttacatgtat
     1261 atcaaaaatg aaaataatat atgattacat gaggaaaaaa catccatttc taacttctag
     1321 tgatgactat tgtaatatag cacttattgc aattcattca aaaaatttag atgaagattt
     1381 agaatacata gaaaaatgtt atgaatttct aaatgaaaat ggtttttata aaggaaatga
     1441 tttacaagca ttatcccaaa ttcttttatt tagtgatgat agaactatgc ttaaatgtaa
     1501 aaagactatt gaacttaaaa aagcatttaa agaaaatgat tgtaaaatga attattatgg
     1561 atatccaata ataggagcaa tttcattatt agattataga gaagatgaaa taatagaaga
     1621 gataaaaaat gtttcaaata aacttaaaga agaaaaaggc tttggaaatt ggtctttagg
     1681 aaaaagtaat agagtaatga ttagttctgc tattgtagct tcaatttatg cagatttgat
     1741 acgaaatgag aataatatag gctctataac taataatata tttttaaata ttgttgttgc
     1801 tattcaaatt gcatgtgtaa tggcagcaac aagtgcagct atagcatctt cttcatcaaa
     1861 ttaataagat ttattgaagt tggatatatt aaatttaata atacaattta tatgtttaaa
     1921 aagaagatag tattacattt taattaactg
//
LOCUS       DAMJVX010000310         1939 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.317, whole genome shotgun sequence.
ACCESSION   DAMJVX010000310 DAMJVX010000000
VERSION     DAMJVX010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.317"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            153..1556
                     /locus_tag="PC407_003645"
     CDS             153..1556
                     /locus_tag="PC407_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2499029.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
BASE COUNT          769 a          248 c          309 g          613 t
ORIGIN      
        1 tatatacata aattcatttt ttcatacatc ttacaaattt atttttattt agataacatg
       61 tatatttttt ccatgaaaaa taaataataa aaaatagtaa atatggaaaa tataaaattg
      121 attaacaata attaattacc tgaggtgtga atatgaaaat gctaaacaac ttttttaaga
      181 aaatcaacaa tccagctcct gtctttgcac agacaaaaat ggaaagagat aatcaaacag
      241 atgattcact ttctacaaaa ccaaagacta catttagaga cgttgctgga ttagacgaag
      301 taaaagaaga actatttgaa atagtcgatt ttatgaagtc accacaaaaa tatcaaaaaa
      361 tgggtgcaaa aatacctaaa ggagtattat tttatggtcc tccaggaaca ggtaaaaccc
      421 tacttgcatc tgctgtagct ggtgaaacaa actcatcatt tttcaatgtt actggttcag
      481 agttcgttga aaaatatgta ggtgtgggag caaaaagagt tagaactcta tttgaaaaag
      541 ctagaaaaga agctccaagt ataatattta tagacgaaat agatgctgta ggagcaaaga
      601 gacatcttga aagtaacaac gaaaaagacc aaacactaaa ccaactttta gttgaaatgg
      661 atggatttaa taaagattcc aatgtattaa taataggtgc taccaataga cttgacttat
      721 tagatgaagc tttacttaga ccaggaagat ttgacagaca cattcatata ggtgcaccaa
      781 actatcacac tagatttgaa attctaaagg tacatacaga tgacaaacct atagacaaat
      841 ctgtaaatct agaactttta gctaaaaaaa ctcatggttt caatggggct catctttcaa
      901 atatagcaaa tgaagcagct atatttgcag ttagagatga cagtgagtgt attacatcag
      961 aacattttga caaggcactt gaaagagtta ttgcagggct agaatctaaa aactctgcat
     1021 tggttgaaaa agagaaaaaa atagtagcat atcatgaagc tggacatgct cttgtaagtg
     1081 atatagtagg aatttgtcct atacaaaaaa tatctatagt tcctagagga caagcattag
     1141 gttatgttct tcaattacct gatgaagata gatatattta tactaaggat gagttgattg
     1201 gaaaaataaa aatattactt gctggaaagg cttcagaaga attaatattt aatcataaat
     1261 ctactggtgc aaaggacgac ttaaagaaag tcactgaaat agctaaccaa atggtttgtg
     1321 aatatggtat gagtaactta ggatttatga ctattgatgg aaatgataag actttcttat
     1381 gtgataaggt tcaaaaggaa gcaaatcgta tagttgaaat ttgttataaa gagactttag
     1441 agatgttaaa ggataatctt gaagatttac attctgtttc taaattctta tttgaaaaag
     1501 aaactatgac tcatgaagag ttaaaagatt tgataggaaa agaagctgta aactaatata
     1561 gttttaaaaa tttgaggctg tctcaaaata gagataagcc tctttttatt gttaaagtta
     1621 taaaaatata tttgatttta tacaaaaaag tgcctcataa attaaaaatt tcattttgag
     1681 acacttacat attttaatta catattttaa ttttgtctat ttacagaata ttatatctac
     1741 ataaaactaa actgctgtaa acttaactac ttccagtaat gtttgaagtg gtataaaagc
     1801 tagtagccta gtttctacac ttataattta tattttacat aactaaattt agtagatttt
     1861 tatactcaca atttagaacg attgaaaact taaaaatttc ttatcagata tctacaactt
     1921 aattaggtat ctatcactt
//
LOCUS       DAMJVX010000311         1903 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.318, whole genome shotgun sequence.
ACCESSION   DAMJVX010000311 DAMJVX010000000
VERSION     DAMJVX010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.318"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(136..351)
                     /locus_tag="PC407_003646"
     CDS             complement(136..351)
                     /locus_tag="PC407_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499030.1"
                     /translation="MHINQTGFISNGLGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVREGSSWAFNFIVQP"
     gene            complement(534..878)
                     /locus_tag="PC407_003647"
     CDS             complement(534..878)
                     /locus_tag="PC407_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499031.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            1005..1388
                     /locus_tag="PC407_003648"
     CDS             1005..1388
                     /locus_tag="PC407_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499032.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
BASE COUNT          715 a          235 c          236 g          717 t
ORIGIN      
        1 taagaaatat taatagtaaa taagcaattg ttactataac gtaggaatta tctttaaaaa
       61 cacaaattaa tttacattct ctaattttaa agtatatgta tatctttgat tttaataaaa
      121 ttcacttaaa atattttaag gttgaacaat gaaattgaat gcccaagaac ttccttctct
      181 tactaatgtt gcaattaatt caaacccaca attgtattga actgaatctc tacctactgg
      241 tttccaacta gaaggtgcat tatatgagaa agaatttgat gttggctctt tcttttgttg
      301 ctgcttaatt cctagcccat tagaaataaa tccagtttga tttatatgca cccttctatt
      361 tgttacacga atctggccgt ctgcaataga ccagtttcca gaaactttac taacggaaat
      421 atattgaaat gctccatctc cttttgatat tgtatatgta actgtaccag atgcttttac
      481 accaccagtt ttatcccatc catcttctga tatacttgct tttctaagag gctttattgt
      541 attagtatca aaaaaactgt cttagaatat atctttgatg ttaaattttt agattgttta
      601 acttctaata attgagttgt tgatatagaa tcaacgtata tggactctcc tgttcttgca
      661 tttttgatta tacctttttc attggaactt ttaacatcag taattccatt tttagccata
      721 tcgtataatt tgtccatgtc tgttacttgt ttctctgaat acattacttt catagatgaa
      781 tcttctacct tactagcact tgcatttgtt ggtaatagta ttgtcattat gagtaaagat
      841 aaaatagttg ttacagattt ttttaaccta taattcatat taatccttac tttttatttg
      901 ttataacaga cattctattg taatagaata taaatccaca atatttttaa aattagtatt
      961 gaataactat tctcatgtta taattattca aaggagggat tgatatgagg aaaaaatttt
     1021 ttataggtat tcttatagtt ttaataatct ctgtgatagc tatacttaat ataaaaaatg
     1081 ataaaaatgt ctttattgga gaatcagaaa attggaaagc taaatttgta actactgaag
     1141 gacactcaaa attagaaata aaatataaaa atagtaaaga tgtagttgaa caaaaattta
     1201 attattccta tcaatttata gatggtgaag atgcagttgt agttgtaaaa aatgaaattt
     1261 tagaagcagg tcaagaaaaa gcaataaaaa tacaaagacc aggtctttat tcaaatagag
     1321 aatctagtta caagctaatt atatcttgga atggaaatga ggaaactata aacttgaaaa
     1381 agaaataacc atattatcaa tttttaaaaa attattattc aaattcttat aaaaagagtg
     1441 tttagaaaaa tgaaacactc ttttttgtat aagtttttga aaactataaa tgaaatatat
     1501 caaaatcgtt cgaaaaatta ctgaaataaa ctcttaaata tattataaat taaggtatat
     1561 tatacatgaa tttattggga gtttatgagt tttaaaattg gaaaccaaac aataagggct
     1621 aaaaatactt taaggaaaat atataaagtt ctataaatta tcaaaaatat ttcgcaaaaa
     1681 ttgataatta ttacaaataa attgataata ctaataatat tagaaatatc gaaattgaac
     1741 aaactaggat taaagctcaa ttagaaaaat ccactaatcc tgagaatgct ttgttattct
     1801 catttattac tgttgttttt tcattatttg ctaatttatt tttcagttca ttaaatgtta
     1861 aatataactc tataaattta attttacagt taatatgtat tat
//
LOCUS       DAMJVX010000312         1892 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.319, whole genome shotgun sequence.
ACCESSION   DAMJVX010000312 DAMJVX010000000
VERSION     DAMJVX010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.319"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            211..1815
                     /locus_tag="PC407_003649"
     CDS             211..1815
                     /locus_tag="PC407_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2499033.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGAANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVLDNNREFIKKYINR
                     FKVGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
BASE COUNT          813 a          205 c          323 g          551 t
ORIGIN      
        1 ttaacaactt ataatatttt ttcaattagt ataaagccta ttaatttgtc ttatttacaa
       61 tgaatagtat ttacttcttg gcaaacaata attaaaaata aaacttttat atgattaaac
      121 tctaaagaaa aagtttataa agaagataat ataattaagc agaaaataat gaaaaaaatt
      181 tattaaagta gacaacgtaa ggagaatttt atgaaagaaa aaatttatta caatggaaat
      241 atcatcacaa tggaagattc aatatgtgga gatgctattc ttataaaaga taaaataata
      301 aaaaagatag gaacaaaaga agaagtattt gctttgaaaa ataaagatac agaaattata
      361 gatttacaag gtaagacatt gatgccatct tttatagatt cacatagtca cttgatagct
      421 tttgctacta ctctaaagct agttccttta gaagatgcaa ccagttttaa agacatagta
      481 aaaaaaatac aggatttcaa agagtcgaat aatataaaaa aaggagactg gataattgga
      541 ttttcatatg ataataattt tttagaagaa aataagcatc cagataagag tgtactcgat
      601 agtgcatctt cagaaaatcc aatactaatt tcacatgctt ctggtcatat gggagctgca
      661 aatacattgg ggttaaaaca gcttggtgtt accaatgaaa cacgtgaccc agaaggtgga
      721 catataggta gagtagaagg cagtgaagaa ccaaatggat atttagaaga aaatgcattt
      781 tttaatgtag catctaaaat aaaacaacca tcatcaaatg agatttttaa ttcaattgaa
      841 aaagcacaga acatatatct aagctatggt attacaacag ctcaagaagg tttaatggaa
      901 gaaaatcaat ttaacatttt aaaagcaatg gcaaatcaaa ataaattaaa aatggatgtt
      961 gtaggatatg taaatttgaa aaaaagtaag tctgtattag ataataatag ggagtttata
     1021 aaaaaatata taaatagatt taaagtagga ggttataaga ttttcttaga tggttctcca
     1081 caaggtaaga cagcatggtt gagtagacca tatgaaaatt cagatgatgg ttattgtgga
     1141 tatcctatat acaaagatga agaagtagaa aagtttatag atatttcact aaaagaaaag
     1201 atgcaactct taactcattg taatggagat gcagcagcag accagttgat agatgcgttt
     1261 gagaaagttt taaacttaaa agagcaaagt agtgaaaata atataagacc agttatgata
     1321 catgctcaaa cagtaagagc agaccagatt gatgatatga aagtaataaa tatgatacct
     1381 tcatactttg tagctcacac atattattgg ggtgatattc atatcaaaaa ccttggagaa
     1441 gatagagcgt ttaagattag cccacttaaa acaactatag aaaagggatt aatttacact
     1501 cttcatcagg atacaccagt aatagcacct aatatgctgg aaacagtatg gtgtgctgta
     1561 aatagaatta caaaaaaagg tatacaaata ggagagaatg aaaagataag cccattagat
     1621 gctttaaagg gtgttactat aaatgcagca tatcaatatt ttgaagaaga taaaaaaggt
     1681 tcaattaaag aaggtaaatt agctaatttg ataatattag atgaaaaccc acttacaatc
     1741 gaccctatga aaataaaaga tattaaagta ttgcagacta taagagaagg agaagtattg
     1801 tattctttga agtagatgaa agttagatat tttataatct ataaaaaata gttttatata
     1861 ataagttagg tagaattaaa taagattaca gt
//
LOCUS       DAMJVX010000313         1882 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.320, whole genome shotgun sequence.
ACCESSION   DAMJVX010000313 DAMJVX010000000
VERSION     DAMJVX010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.320"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            158..1738
                     /locus_tag="PC407_003650"
     CDS             158..1738
                     /locus_tag="PC407_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDF2499034.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
BASE COUNT          733 a          168 c          370 g          611 t
ORIGIN      
        1 aataattgtt ttaattcaaa attttttgtt attttaaatt atttcaaaac tcttagtttt
       61 agtgatttta tttatgatat ttatgggatt aaaagatttc aatagatatt tttggtaagt
      121 atttaagaag tctttaactt tataggagtg ataatttatg ctaataggta catctaaatt
      181 aagcagaaat gttgatactg catgtgtaga agaagtaggg attccaatga ttgtattgat
      241 ggaaaatgcg gttatatctg caatgaaaaa tatggatata gacatgtata atagttatac
      301 agttgtatgt ggtgttggaa ataatggagg agatggtctt ggaatagcaa gacatctaaa
      361 tacatatgga aaagaagtaa acgtattttt agtaggtaat cttgaaaaac taagtgaatg
      421 ctctaaaata aattacaatg tacttttaaa tatgggaatt aatattataa atattaataa
      481 aaatgattta agtgaaaaaa attatggaga tgaaaatgaa gtagataaaa ttattaaagt
      541 tgagaaacaa aatattataa aaaataaaaa tttaacaatt tttaaagata gagtatctaa
      601 aagtgatgtt gtagtagatg ctatatttgg gactggatta aaacgtgata taactggtat
      661 ttttaaagaa gttattgaca tagtaaacga taatagcaaa aatacatatt ctatagatat
      721 tccttcaggt atagatagcg ataatggaaa tgtattagga acttgtatta gagcaaacaa
      781 aacgattagc tttgagtttt ataaaagagg ttttctaaac tatgacacta gcacacttat
      841 aggtgatgta gttgtagaaa agataggtgt tccagatttt attacaagaa aataccatga
      901 tgaagagttc ataactgata agacttttgt aaaaaataat attagaagaa gagataagca
      961 tggttttaaa agtgattttg gaaaagtatc tatagttgca ggttcaaaag gattttatgg
     1021 tgcaagtttc atagcaactg aatctgctgt taagagtggg agtggacttg taacattaat
     1081 tagtagtgaa gatgtgcaag aaaaggtatc tattagactt acagaagcaa tgactttaaa
     1141 ttttgaagaa gaaagattaa ataaattact taattctagt gatgcaattg gttttggtcc
     1201 aggtatggga gataatagtc aaacatttga taaattactt aagatagtag aaaattctaa
     1261 ttgcccaata gtattagatg cagatggatt gaacgttatg aaggacagat gttataagtt
     1321 tttagaatgg aaaaatagat ttgtaattac acctcatttg ggagaaatgg ccaggttgac
     1381 aggagattca attgggtata taagagaaca tagagtagat gttgcaaaag aatttgctca
     1441 aaaatataat ctggtggtgc ttttaaaagg ttatcaaact gtcataacag atggtaaaaa
     1501 aacatatata aatcctacag gaaatagctg tatggcaact ggtggaatgg gtgattgttt
     1561 gcttggaatg attacttcat ttataggtca aggtatggat atattggaag ctacagttag
     1621 tggagcatat atacatggct atataggaga taaattgagc aaagcaatgt atacggtaaa
     1681 tgctacggat ttaataagta acatttcatt aacaatgaaa gagttgttgg atatataatt
     1741 gattaaaaaa tagctacgca cacttgtgac tttagtcatg agttagtagc taaaatagtc
     1801 agcatatagg gaaacctata tgtagagata ggatagaacc tatccaatac tacttgaatt
     1861 gctaaaatcc cctaaagcta ac
//
LOCUS       DAMJVX010000314         1832 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.321, whole genome shotgun sequence.
ACCESSION   DAMJVX010000314 DAMJVX010000000
VERSION     DAMJVX010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.321"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            33..1688
                     /gene="ilvD"
                     /locus_tag="PC407_003651"
     CDS             33..1688
                     /gene="ilvD"
                     /locus_tag="PC407_003651"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDF2499035.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIEFNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
BASE COUNT          702 a          214 c          397 g          519 t
ORIGIN      
        1 aaaatttaaa aaagtaagaa agaggtaata ttatgagaag tgatataaaa aaaggaatag
       61 aaggagctcc aaagagagcg ttaatgtatg gaatgggtct tactaaagaa gaaattgaaa
      121 gaccattaat aggtatagta aatgcacaaa atgaggtgat tcctggtcat ctgcatcttg
      181 atgaaatagc agaagctgca aaaaatggag taagaatgag tggtggtttg ccattagaat
      241 ttcctgctat aggggtatgt gatggtatag ctatggggca tgtcggaatg aattattccc
      301 tggcttcaag agaattaata gcagattcaa tagaagctat ggctatggca catggttttg
      361 atgctttggt acttatacct aattgtgaca agatagtgcc aggtatgtta atggctgctg
      421 caagacttaa tataccaagt attgttgtga gtggagggcc tatgcttcca ggtaaaaaaa
      481 atggaaaagt atatgatttt aactctgcaa tggagggtgt tggagcatgt aaagatggaa
      541 cagtaagtga agaagaatta gaagagttag ccatgaactc ttgtcctgga tgtggttcat
      601 gctctggact tttcacagca aatagcatga attgtttaac agaagcttta ggcatgggta
      661 taccatataa tggtactgct gcgtcacatt ctggagaaag aaaaagaata gcaaaatatg
      721 caggtatgta tgttatggag ttacttaaga acgacataaa acctagagat attttaacaa
      781 tagatgcttt taaaaatgct atagctgttg atatggcaat ggctggttct acaaatacag
      841 tacttcactt acctgcaata gcttatgaat caggaataga gtttaactta gatttttttg
      901 atgaaataag tgaaaaaact ccttgtttaa caaaattaag tccaagtgga aaacatcata
      961 ttgaagattt acatatggca ggaggaatac cagctataat gaacgaactt tcaaagataa
     1021 atggaataaa tttagattgc aaaaccgtaa caggcaagac tataggggaa aatataagaa
     1081 attgtgaaat agaaaatgaa gaagtaatac atacattaaa gaatccatat agtaaccaag
     1141 gtgggcttgc aatattgaaa ggaaatcttg ctctaaatgg agctgttgta aaaaagtcag
     1201 cagttgcaga agaaatgtta gttcatgaag gaccagcaag agtttttaat tcagaagaag
     1261 aagctgtaaa tgctattttt ggtaaaaaaa taaacaaagg tgatgttata gttataagat
     1321 atgaaggtcc aaagggtggt ccaggaatga aagaaatgct atctcctaca tcagcagttg
     1381 caggaatggg acttgataag catgtagcac ttcttactga tggtcgtttt tcaggagcaa
     1441 ctagaggagc atctataggc catatttctc cagaagctat ggaaggtggt ttaatcggac
     1501 tagttgaaga aggagatata atttctataa acataccaga caaaaaatta gagttaaaag
     1561 tagatgaagt tgaaatagaa aatagaaaat taaaatttaa acctttagaa ccgaaaataa
     1621 agcatggata cttaagtaga tatgctaaat tggtaacatc agcaaataca ggagcagttt
     1681 taaaatagtg aactatattt acgaaaatta gaaaaaatta agaaaatatt aattggatat
     1741 cttaggtgtt atagcactaa tgaggtatcc tttttatttt gtatgaaaaa tatactgttt
     1801 gtatacaaaa atattaaaga tattttagta ag
//
LOCUS       DAMJVX010000315         1786 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.322, whole genome shotgun sequence.
ACCESSION   DAMJVX010000315 DAMJVX010000000
VERSION     DAMJVX010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.322"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            49..495
                     /locus_tag="PC407_003652"
     CDS             49..495
                     /locus_tag="PC407_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2499036.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEG"
     gene            497..1591
                     /locus_tag="PC407_003653"
     CDS             497..1591
                     /locus_tag="PC407_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HDF2499037.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVIKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1679..>1786
                     /locus_tag="PC407_003654"
     CDS             1679..>1786
                     /locus_tag="PC407_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2499038.1"
                     /translation="MKSLVLYSSLTGNTKKIAYAIYDEIQEEKDIKDVNE"
BASE COUNT          727 a          175 c          324 g          560 t
ORIGIN      
        1 atagttgata tagtcaaaca tattataatt atagaaggga aatttaagat ggattttaaa
       61 aatttacagt atgaaagttt gtcatatatt atttgtaact tgcaaaaaaa ctttaaactt
      121 tattgtgaaa aatgtttaaa accatataaa cttacgaatg gtctatattt ctatttaatt
      181 tatattaata aaaataggaa ttgttctttg aatgatgttt ctacagaatt tgaagttgat
      241 aaagctcata caacaagaac tatatctagg ttagaacaag atggatacat agaaaaaatc
      301 caaaacccta atgatagtag agcttttcag ttgagagtaa cagataaggg tgaggaagta
      361 ttaggggata taaaaaacat attttcaaag tgggataacc acataaaaaa agagttctca
      421 gatacagaat ataaagaact ggtaaagaat ttacatgtgg taaaggatat aaaaacagct
      481 gtggaggaag gataggatgt attgtaattt attaaaacca attaaaattg gaaatttgga
      541 attaaaaaat agagtatctt ttgcacctac aagcatgggt cttaaattgg aagaaaaaat
      601 taaaaagttt tctgatattg ctaagtcagg agtagctctg attactctag gagatgtatc
      661 tattagacct agttttcata aggtagctat aagcctatcg gatgaagatg gagtcattaa
      721 atacaagaaa attgtagatg aaatacacaa ttctggtgca aaggtatcag cacaactatt
      781 ttgttcagat tatgatgtta atttaattaa agatactatg aagatgggaa ttacttcaca
      841 tgatgagata aaaaaaataa tgaatgacgg agtaaaagat tacataacaa atatgcccaa
      901 agaagaaatt aaaaatatta taaatttatt taaagtaaca gcattgaatg caaaaaaagc
      961 aggttttgac atgattcaaa ttcatggtga taggcttgta ggaagttttt catcaagtat
     1021 atttaataat agaaatgatg aatatggtgg aacgtgtgat aatagaagta gatttgcaag
     1081 cgaaataatt agtagtatcc gagatgaagt taaggacatt ccaatagact ataaatttgc
     1141 aataagacaa gaaaatcctc attatggaaa tgcaggagtt ttactttcgg aagttgagta
     1201 ttttgttaaa aaatttgaat ccctaggtgt aaacagtttt catgtaacac ttgcaaatca
     1261 ttcaaaatta gaagatacta taccaaccaa taatcatcct tactttaaag atgaaggctg
     1321 ttttttatat ttagctgatg aagtaaagaa acacacaaat cttccagtat gtggggttgg
     1381 aaaattgtct agcccagatt ttatagaaag tattatttca aataatagag tagatatggt
     1441 ttctatgtca agacaattac tagcagatag taattggtta cagaaagtaa aagatggaag
     1501 agtagatgag attaaaaagt gctgttattg taataagaag tgtgcagatg ctttacaaac
     1561 acgctctcag tttggatgta tactagatta gaatatcttt ataaaaataa ataatttgtt
     1621 aaggttaata tatttttggt gatttagata agtatttaga taaagaggag gagacatcat
     1681 gaaaagttta gttttatatt caagtttaac tggaaatact aaaaagattg cttatgctat
     1741 atatgatgaa attcaagaag aaaaagacat taaagatgta aatgaa
//
LOCUS       DAMJVX010000316         1751 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.323, whole genome shotgun sequence.
ACCESSION   DAMJVX010000316 DAMJVX010000000
VERSION     DAMJVX010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.323"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            137..1186
                     /locus_tag="PC407_003655"
     CDS             137..1186
                     /locus_tag="PC407_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2499039.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWVQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            complement(1336..1683)
                     /locus_tag="PC407_003656"
     CDS             complement(1336..1683)
                     /locus_tag="PC407_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2499040.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
BASE COUNT          646 a          238 c          280 g          587 t
ORIGIN      
        1 aaaaaactaa acataaaact gaaaattatt attaagtttt tgtagaaata ttataagcat
       61 agatacattt ttgtaacaac tacaataaat ttttaatact atatactatt cgtatgtctt
      121 atgggaggta tttattatgt ttaaaaaaat agtcgcaata gcaagctcta tcattgtaag
      181 tacaatatgt ctaactggat gtgattttaa ctctaatgac aaaaaagatg aatcttcttc
      241 gtctaaaaat ctggataaag taactatagc agaagttact cactctgttt tttatgctcc
      301 acagtatgct gctattacga aaggattctt tgaagatgaa ggtataaaga ttgaccttat
      361 aaatacccaa ggtgcagata aaactatggc agctttgatt tcaggtgaag caaacgtagg
      421 tttaatgggt cctgaagcat ctatatatgt atataatcaa aacaaaaacg attatgcagt
      481 gaactttgct caattgacaa aaagagatgg tagttttcta gttgctagga aaaaaatgcc
      541 taatttctct tacaatgatt taaaaggaac agaaatatta ggtggtagaa aaggtggagt
      601 tccactaatg acttttgaat atgttctaaa agaaaaaggt cttactattg gagaaaatct
      661 aaaagcaggc aatgttaatg taagaactga tgtacaattt ggtgtaatgg ctggagcttt
      721 tgcaggtgga gagggtgact ttacaacagc atttgaacct acagcaacag caatggaaaa
      781 agaaggtagt ggatatatag ttacatctat aggaaaagat tctggagaaa taccttttac
      841 agcatactct actacgagat catacatgaa aaacaataaa gatttaattc aaagatttac
      901 aaatgctgtt tataaaggtc aattatgggt tcaacaagca tctagcaaag aagtagctaa
      961 ggctatgcaa cctttctttg aagatatatc tttagaagat ttaacaacag ttgtagatag
     1021 atataaatca atagatgcat ggtctcaaaa tcctgtatta aaggaggaag gtttgaagaa
     1081 attgatgact gttatgaaag aagctaaaga actagataag gaagcacctt ataaagatat
     1141 cgtaaatact gagtttgcaa acaatgctat aaaaaatatt aaataaaata tattagtttt
     1201 agctagatat tttcttgttt ttataatgaa aattaacaaa aatataaaaa gagcctttta
     1261 aaagactctt tttatatttg agattaaagt ttatataata aaatagatat taaagtacta
     1321 actgtactta tatatctatc ctctgtattc tgctccagca ttaatttttg taatttgaac
     1381 ggctaccatt tcttcattgc ttccattagt aatttcatgc cactctcctg ttggtacaaa
     1441 tacagtatcg ccgtttttta catcacattc atgctcacca agtttcattt taccttctcc
     1501 ttttaggaac acaaatattt gttcaccatt agggtgtcta tgagcattaa gaacttgatt
     1561 aggtttaaaa taataaactt ctgaagttat atcgttacca gcaaatactg gtatttttgg
     1621 aactgctgta tcattatatt gagctaagct tccgtctaat agattctttt tattttcatg
     1681 catactagaa atacctcttt tctatattat agatttacta ttattatacc aatataagaa
     1741 aatagtattc t
//
LOCUS       DAMJVX010000317         1719 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.324, whole genome shotgun sequence.
ACCESSION   DAMJVX010000317 DAMJVX010000000
VERSION     DAMJVX010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.324"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(260..1588)
                     /locus_tag="PC407_003657"
     CDS             complement(260..1588)
                     /locus_tag="PC407_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2499041.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGDKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGIKR"
BASE COUNT          616 a          325 c          200 g          578 t
ORIGIN      
        1 tatagatatc acttagtttg aaaaaaaaac actttaaaat aaagtatctt aaagcaaata
       61 attttttaga tgtttaattt tataattcaa aaactaaaaa taaagtgaat accttaaaat
      121 aaacttttta gttcatagaa cactcttttt ttataaaata aaacatctct ttaccctttt
      181 aacaataaaa aagagactta tctctatttt gagacagtct cttttatttt aattataaag
      241 catttgtaaa ctacttggtt tatcttttta ttcctctagt catataagca tacactggta
      301 agcctatagc tgttattaca agacctccta tagatatcat agtagttttc atccctgcaa
      361 aacacaattg attaactaca acaaatatac cacttgctat agctattata ggaacaatag
      421 gatataatgg aactttatac tctcttggta tatttggatg agtctttcta agtttcataa
      481 cccctataaa agttaataca tagaatatcc aagtagcaaa tacagctaag tcagttaata
      541 aattaaattg acctgacaaa gcatatatag atgcaataac agatactaat gctattgcat
      601 ttgctggaac ttgagcagaa tttaatttag aaaatatacc atactttggt aaagtcttat
      661 ctgtagctaa agtatatgcc acccttgaac cagttagtaa aaatccattt aatgctccaa
      721 acactgaaat taatatacct acagatatta tttttccacc cattgaacca aatataactt
      781 cagcaactgc tgatgcagga gaagcataat ttgctaattc attagctggt aatacccaaa
      841 gatacgctaa atttattata aaataaacag ccataacaac tgacaatcca cctattatag
      901 cttttggaag gtcttttcca ggattcttca tttctccagc taaagtacca acattcatcc
      961 atccatcaaa agcaaataat atagcaatta aaacttgacc tagtacacta cccaggctta
     1021 atccttcacc aaccataggt gtaagtattg gattatttcc accaccttta ataaatccaa
     1081 caaccattat taaaataagt ggtattaact tacaaattgt agagacagtt tgtatgactc
     1141 ctccagtttt tgaacctaac atattaagcc ctgcaactat tactataatt ccaattgcta
     1201 ttggaagtaa aagtgatggg ctaccaatta gtgctgatgc ttgttctcca aatataacac
     1261 ctaatgctgc catcatccct ggataaaata atacaatttg catccatcca gttaaaaaac
     1321 ctaatttgtc tccataaatt tcttttatat ataccatcat tccaccagtt tttggtatcg
     1381 caactgatac ttctgcagct gtaagaccag ctgttatagt tatgattcca gctattaacc
     1441 atgccaatat accaagacct ggagctcctc cagttaatgt atagactgct tgtggtttaa
     1501 aaaatacgcc cgctcctata acagaaccaa caacagttga taatgctgct gatacgccaa
     1561 gagtcttttt cagttcatta cttcccattc ttttcctcct cattaatcct atttaatttt
     1621 aatttcatag ttaaataagc atatattatg ccaatatata gaaaaatagc ttttttattt
     1681 attaaaataa aataaactct acttagcttt cgtaatctt
//
LOCUS       DAMJVX010000318         1696 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.325, whole genome shotgun sequence.
ACCESSION   DAMJVX010000318 DAMJVX010000000
VERSION     DAMJVX010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.325"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            211..549
                     /locus_tag="PC407_003658"
     CDS             211..549
                     /locus_tag="PC407_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2499042.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            571..888
                     /locus_tag="PC407_003659"
     CDS             571..888
                     /locus_tag="PC407_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2499043.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1038..>1696
                     /locus_tag="PC407_003660"
     CDS             1038..>1696
                     /locus_tag="PC407_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499044.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     L"
BASE COUNT          746 a          160 c          257 g          533 t
ORIGIN      
        1 gtagtatggc tatgaaatta tgaaaatatt tctctaatta aaaatatgat ttatccatat
       61 ttgtttattg tagatacaga ctatacaaaa ataaatagca cttatttcag tataaaaaca
      121 tatatagtct atatttagac taaaaaagta taaatagtct atataaaaca acttaaacta
      181 tgaatataat ctaaagtgga gggataataa ttggcaaata taggaaaaat aataggagat
      241 agaataaaag tctatagaac aaaattggga tatagtcaag agtttctagc agaaaaggca
      301 ggtttacatc caacatatat tggtcagatt gaaagagctg agacaaatac atctatcaat
      361 attataatga aaattgctac tgctttagac ataccacttg aattgttatt tgcaaatatt
      421 attacaacag aaggcgttga taactctgta cctttagaat gttatgaaat gattaataaa
      481 ttaactgaaa aagaacaaat tgcaatgcta gagttaataa agtgcatcat taaatacaaa
      541 gaaatataag tgagggaaat agtaacaata atgaattata taggtaaaag acttaaagaa
      601 gaacgaaaaa aagcaaattt aacttcaaaa gaatttgcaa atatggttgg agtatcgcct
      661 tggtatataa ctcaaatcga aagtggtaaa aagaatccaa gtcttaaaac attcataaaa
      721 tttgtaaata tattaaatat ttctgcagat gttcttataa aagatattac ttccactgga
      781 aaaacatatt tagaaaatga tataaatgaa gagttaaaag acttaaattc aagagaacta
      841 aatctgatat cacagattat agacatcata aaaaagaata aaatataatt ctttatattt
      901 tatataaaaa aaaaggttat ttgaaataaa taaccttttt ttgtcttttt gtgttaacat
      961 tgtcttatag tgctaatgag tactattaga ccatgttcta tggccttatt ttaatataaa
     1021 acgattggag ataggctatg attgtgattg aaggtagtga taaatttaag attgcaaaag
     1081 aatatattga tgtagaatat actcttttta gcaaagtaac ttttaggtat gaaaagttga
     1141 aatttaaaga taatgctgaa ttggaaaaaa ttaagatgtt taaatataaa aatggctaca
     1201 tccctaataa attaaacctt tcttttggat atggattctc ttcttataaa aagcaaataa
     1261 ttagagaaac tgtagatact ttaagattga cagaaatttt ttcaagcgag aacatagaag
     1321 atattaaatt tataaaagat ggtacaaaaa aattagaaat tagcatagag aaagttgtga
     1381 aatttaaacg tcgaaaaaaa agaaattatg tttgttgcta ttgccctgat atgtatagag
     1441 atataaaact cgacaaagaa tctatcaata agatatacaa cagaaaaata aaaatagaaa
     1501 gagaagttaa tatttttgaa gatgaggatg ttataataaa caagaaagta ttgaagtttc
     1561 caaagtcttg gacaaagaat atgcaaaaat attggttaag tgaaaataag tatcccatac
     1621 attctactgt aattgatgat gatagatata aatgttgtaa tgtacaatat acaaaaaata
     1681 gagtgataat attata
//
LOCUS       DAMJVX010000319         1682 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.326, whole genome shotgun sequence.
ACCESSION   DAMJVX010000319 DAMJVX010000000
VERSION     DAMJVX010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.326"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            158..598
                     /locus_tag="PC407_003661"
     CDS             158..598
                     /locus_tag="PC407_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2499045.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLETQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVSLTPKA
                     KELWVTRYTCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(783..971)
                     /locus_tag="PC407_003662"
     CDS             complement(783..971)
                     /locus_tag="PC407_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499046.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
BASE COUNT          641 a          239 c          167 g          635 t
ORIGIN      
        1 catttttttc caatatatat aaatatcata tttttctatt agctaaaaat aattacttta
       61 tatttttaca tgttagtata ataagacata aaaacttgac atactgttgt cataattact
      121 ttgctataat taaaagtaaa aatggaggta aattataatg tatgaacgta tgcttgataa
      181 aagcaataaa ccctgtttgt ctcaaataat agaatatata ggtaaagatg gatatgcacg
      241 attgtgtaag ttggaaactt acttggaaac tcaatataat ttatcaagag aacttcgatt
      301 tccttttgga aacaattatg gttggggtta caaatatagc cataaatctt ttcatttatt
      361 ttatgctttt tttgaaaaag gtgcatttac tgttctgatt cagataggtg acaagcaagt
      421 tccagcagta gaaaaaacgt tggtatcttt gacaccaaaa gctaaagaac tctgggtcac
      481 tcgttatact tgtggcaata atggaggttg gattaagtat cgtattttag aagatgcaga
      541 cttacttgat attattcaat atataagagc aaaaaaatct ccacctaaac aaaattaact
      601 tttatttttt gtgatttaaa tatagtgtat ataaattcca ttctttccat attaaaaaaa
      661 taatataaaa aatatctatt agtatatctt atttagtatg tcttattcta ctttttacag
      721 ataattaaaa agttttatca taaataaatc ataactaaac atcctagtaa atcaaaattc
      781 atttattctt ttttattaga atttttgata acagtcccta tacatatgcc aattatcatt
      841 ccaaaaatta gaccataaca cattgtgata cccacatttc ctttagaaat acctatagca
      901 caacctatag ccattccaag tatcatgcct ataaacatac atataataac taaattgttg
      961 tttttattca tagtttttct ccattcaata tattttaaat tgcctagctc gccttcttag
     1021 cttaaaacaa agaaatataa attaactttc tttcattcta atatctgctt gtataaatgt
     1081 caaacaacac atttcttgtc ataacctcct ctttttgaag taagtacaat ctcccagaca
     1141 caataatatc aatttcctaa aaccatttaa gtaactttta tatttacata atatttacaa
     1201 tcataaacta tattgttatt catttataca tcataaacac tccctcctaa tcattattat
     1261 acttaaaata atgttcattt ataataatat atctaacgca ctttaattga aaaaactaaa
     1321 actttgcctt tcattcccat atataaataa gtatactttt tgatgctcta ttacaaatca
     1381 actaaattaa ttgatataaa ttatcatttt ctgttgaaaa taccatttac acatgctata
     1441 cttaattgca taaatggtaa taaatgtcat atgattttac aaatctatta ataacaatta
     1501 ttacttttac tgtattttaa agtttttgga ctaaatatca agaactttct aaaatacaaa
     1561 gtacaaaaaa tttatataag ttataaattt atatgaacta gataagctca aacaatatgt
     1621 tatgtataaa ctgtgtagta ttcggttata aatttatata aattatataa gctatataaa
     1681 gt
//
LOCUS       DAMJVX010000320         1641 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.327, whole genome shotgun sequence.
ACCESSION   DAMJVX010000320 DAMJVX010000000
VERSION     DAMJVX010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.327"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(102..1583)
                     /gene="cls"
                     /locus_tag="PC407_003663"
     CDS             complement(102..1583)
                     /gene="cls"
                     /locus_tag="PC407_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2499047.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT          597 a          246 c          191 g          607 t
ORIGIN      
        1 aaaaataaaa aaaatgagct aaattcaata aaaaaaaaat aatacatata aaaaacttta
       61 aaaaataaac caaaatgtaa aatttttata aaataaaaga tttataaaac tgctgataat
      121 agtcttgaaa ttgattcctt gatttttata taagtagacc tagaattata tatatctaat
      181 gttatttcct tagaatttaa aatatcattt tcaaatataa ttctttgttc caatgctttt
      241 tttgaagagt acataaatgc attaacttcg aaattaagct caaaacttct gatatccata
      301 ttagctgttc ctatagagca tatagagtca tctataacta ttgtcttagc atgtaaaaat
      361 gcattttgac cataagtgta gattttagct ccaaacttta aaagttctcc agcatatgaa
      421 tatgatgccc agtatacaaa gggatggtca ggtttagaag gaatcataac tctcacatca
      481 acaccagata aacaagctat ttttaaagta tctataaaag ttgagtcaag tattaaataa
      541 ggactctgta tgtatatata ctttctagct ttctgaatca ttttaaggta accatattta
      601 atctcatcaa gttctgatat atctggacca cttgatacta tctgaatccc aatattttca
      661 gaaggatgat tagaatttgt atcttcttct ataaagtact tttctaaatc taaatcttct
      721 ttagttgtat atctccaatc aaggatgaat ctcatattta ggtcaattac gcaatcacca
      781 gtaagcttag tatgtgtatc tctccataga ccaaattttg gatttctacc taaatattca
      841 tcaccaacat tatatccacc tacaaatcct accttaccat caatgacaac aatttttcgg
      901 tgatttctat agtttaagtt aaagtttaca attttcaaga atgatgggaa gaagcttcca
      961 actttaacac cactttcttt aagtctggaa agtgtactat tttttagtgt tctaccacca
     1021 actgcatcaa ataaaagtct tacttcaaca ccttctttag ctttgtctat aagaatgtct
     1081 attatttcag ttccgatttt gtcatctttg aaaatgtaaa attgtatgtt tatatatttt
     1141 ttagcttttt taagttcttc aagaagactg ttaaaaaatt gactagattc agcataaatc
     1201 cacacatcgt tattattggt ataatgagca ttatttgaat tagccaatgc ataaatcata
     1261 tccttatgtt gatgtatatc tgaatcaatt tcagatgtag attggagttt aacctgagta
     1321 tctagtatat tacttttgat tatcttatca tccttttctt tcaatctaaa catattgttt
     1381 tttgaaatat ttctgccaaa agctatataa agtatgaatc caagagcagg aatcaatact
     1441 aatataagaa gccatgttaa agttgtattt atgtttcttt tttctctaaa gataaggttt
     1501 agtataacca tgaaattaat tatatatatt gtagtaactg ctatttcata tattgacatt
     1561 tcgaaaaatg atatatcaaa cataaaattc cctccttcaa atatttatta aaataagtaa
     1621 ttatatataa taacacaaac a
//
LOCUS       DAMJVX010000321         1629 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.328, whole genome shotgun sequence.
ACCESSION   DAMJVX010000321 DAMJVX010000000
VERSION     DAMJVX010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.328"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(139..1530)
                     /locus_tag="PC407_003664"
     CDS             complement(139..1530)
                     /locus_tag="PC407_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2499048.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
BASE COUNT          585 a          306 c          183 g          555 t
ORIGIN      
        1 aattataatt ttaattataa tatctatttg tgtaaagctt tttttcataa tcaaagagga
       61 aattacattt aattttatat ttaaatgtaa cttcctttat ttgaagtaat tcatctattt
      121 acaataattc gtcatgaatt atttttcttt tgttagtaaa tccatagcca ttaatcttgt
      181 taatacattt cttattccca ataatattaa accaaatatt attaaaactg ttgctattcc
      241 tccaaatatc tgtaaaaaac caagtgtggc tgtaaaacca gcaatctttc ctgataataa
      301 acttgcaaaa aaacttgtta gataccaggc tcccatagat aatgtagcat acttagctgg
      361 agctaattta ttgaacattg caagtcctat aggagataag aataattcac ctactgttgt
      421 aaatatataa gtcaatacta taaacaatat actagccttt accattccat ctggaccatt
      481 agctaatgta gcaaatacca taactataaa cccaacaccc attattatca ttcctaatcc
      541 cattttaact ggaatagaga aatcacctct tttggtatga gataatttaa cccatagggt
      601 agctaatact gggcataaag ccacacataa tatagcattt agtgatgtaa gccatgctac
      661 tggcatttca aatccaaata gagttctatt tacattatcc cttacataaa tcataaaaga
      721 actttgtgtt tgatagtatg ctgtccagaa taagaccaca aataaaaata aaataaccat
      781 tgctattatc ctatttttct ccaacttagt aagtggtcca tactcaattt tttcaccttt
      841 attaccttta gtcacaggat attttccgac ttcatttaat aattttggtg caagaagtgt
      901 aaatataatc atacctaaca tcatgcctat accacaaatt aaaaatgcca tcttatatcc
      961 ataagatact atatctccac ttgctcctct tactgcaaat acattatcag aaagcaatcc
     1021 acatattatt ggagctataa atgctcctac atttactgcc atatagaata aactgtatgc
     1081 tgcatctttt cttgacacat cagttttagg atatatatcc ccaactatat ttgaacaact
     1141 tgatttaaaa aaaccatttc ctaaaataat tattgataga cctacccaaa ccatggcaat
     1201 agatgtagat gaagcaaata gtacgaaatc tcctattgct attaaaagtg cacctataga
     1261 aatacatttt tgttgaccta aaactttatc tgctaaccag ccaccgacta atggtgtaag
     1321 atatataaga cctgtaaata agccaaacat ggatgttgct tcttgagtag aaagacctaa
     1381 accaccttga gctacacttg ctgtaaaaaa taataccaat aaagctgtta aaccataata
     1441 actaaatctt tcccacaaaa ttgtcatgca aataagccaa aaactcttcg gatgaccact
     1501 ttttttagat acgctttctg tacttgacat aaaaaaacgc ccccttttgt tttatagttg
     1561 aatttaatat taatattgct ttaaccattt tagcacatac ttataatatc atcttttcta
     1621 tgtttactc
//
LOCUS       DAMJVX010000322         1621 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.329, whole genome shotgun sequence.
ACCESSION   DAMJVX010000322 DAMJVX010000000
VERSION     DAMJVX010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.329"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            41..775
                     /locus_tag="PC407_003665"
     CDS             41..775
                     /locus_tag="PC407_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2499049.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(917..1408)
                     /locus_tag="PC407_003666"
     CDS             complement(917..1408)
                     /locus_tag="PC407_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2499050.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT          607 a          196 c          237 g          581 t
ORIGIN      
        1 agtgttgcta agtgaatata tttaaaatgg aggaaacttt atgaaacaaa ataaatatga
       61 tgatgataga ttttttaata aatacagtaa aatggaacgt tctacaaatg gattagcagg
      121 tgctggagaa tggcatgttt taaaaaaaat gttacctgat ttcaaaggaa aaagagtttt
      181 agatttagga tgtggatttg gatggcattg tcaatatgca gttgagaatg gagcaatttc
      241 tgctgttgga gttgatattt cagaaaaaat gttaaaagaa gctagaaaca aaacgcaact
      301 tgataatata aattacatat gtatgcctat tgaagatatt aattttccaa aagatagttt
      361 tgatgtagtt attagttcct tggcatttca ttatattcaa tcatttgaag atatatgtaa
      421 gaatataagt aattgtcttt caaataaagg taattttgta ttttctgttg aacatcctat
      481 atttactgca tatggaagtc aagagtggta ctgtgataaa gatggcaata atctacactg
      541 gccagtagat agatattttg aagaaggagt acgtaagtca aattttctag gagaagaggt
      601 tataaaatat cataaaacat taacaactta tttaaatacg ttaatgaaaa caggatttga
      661 aatattagaa attattgaac ctcaaccaga agaaaaactg ttagatacaa ttcctgatat
      721 gaaagatgag ctacgtagac ctatgatgtt acttgtatct gcaaaaaaaa gataagtaaa
      781 tataaataat cattattgta gaaaaaaatt gtagaaaata attacactaa tggagatgat
      841 tataccagtg gtattaatta caccagtagt aaggaggcaa aagcattcgt tgcttttgcc
      901 attttttaag ttgcaattat acttctacgt agtatatttc gtttccattt tcttctataa
      961 gctttttcat atcttcaaat tttaaaaata cagtagcagt attatcattt ggatgtatac
     1021 ctattttatc catttttaat aaatccttat caaatacaac ttttacaaca gcctctttat
     1081 catttaatat accaaaagga gtcacagctc ctttagtaag tttcaagtat ttttgaagtc
     1141 tttcttctga tgcaaaacta agaggtttac tatcaatttg ttgacgaata tcttttaaat
     1201 ctgctttttt gtctttacct aatacaacta aaaaatgttc ttttccttta gcatcacgaa
     1261 gaaataggtt ttttacaaca aattccatat gtaatgtttt taattcttcc atttcttcaa
     1321 ttgtataaac tggtggatga tttacagcct cataaggtat gcttaactca tctaattttt
     1381 tatatacttc ctgttgttga ttgttcatga tttcctccca ttagaaattt attttatata
     1441 taaataatat tatcacagaa aactttaaaa gaaaacgttt tatataatat agacaataga
     1501 ttatattttt ataaagaagc aagaatactc aataaaactt aaagaaatta aacaatttct
     1561 aaaaatacct tgaaaataga accttatttg ccatttataa attatataaa aagggattat
     1621 a
//
LOCUS       DAMJVX010000323         1620 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.330, whole genome shotgun sequence.
ACCESSION   DAMJVX010000323 DAMJVX010000000
VERSION     DAMJVX010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.330"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            60..689
                     /locus_tag="PC407_003667"
     CDS             60..689
                     /locus_tag="PC407_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HDF2499051.1"
                     /translation="MDENITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMI
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            757..1590
                     /locus_tag="PC407_003668"
     CDS             757..1590
                     /locus_tag="PC407_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HDF2499052.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPLGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
BASE COUNT          618 a          210 c          243 g          549 t
ORIGIN      
        1 aataataaga aaacttttta taaatacact tttaattgtt taaattggag gaaaaaatta
       61 tggatgaaaa tataacaaaa aataattata atgttccatt tgactatgtt cgaaaaaaac
      121 tcaaagaatt agaccctcat acaattagta atttaagtaa tatacctttc aataatgatt
      181 taaatagatt cacactcaat ttttttgata gacaatatat tatagactat ccaagtggag
      241 aagtttggaa tagtaatggt agcctttgca ataactttga gcttaaaatc ttgatagtta
      301 gatatttaat caatgctaaa ggtatcccta gaactaataa atatgtaact tttaaagaaa
      361 taccaggagg caatgtttat tatccaaatt ttttaaacag gactctatat aggctttcag
      421 aagtttttac atctcaaata gacaaatata agttagttat ggagaaaatt ggagctgaaa
      481 aagtaaacat gggagattta gcttataggt ttacatatat gggtaataca tctatgatat
      541 ttatactttg gtttggagat gatgaatttc ctccaaattc aaatatatta tttgattcta
      601 atatagttta ttacttcaat gcagaagatt tagcagttgt tcctgataca gctataaatg
      661 caattttaaa taaaataaat caattataaa tataaataat attataataa agtttttgat
      721 agatttaata gaatataata tgaaatgggg taaaaaatga atgtagaaaa tcaatcttta
      781 tcagatattg aaataatatt atcacacagg tatgataatg gtgcagacta ttggactaca
      841 cctgataagc gtttaataaa aggttctcct ttctctactc ttgatagtgt actttatcta
      901 ttagagttag gtgtgaaccc tgatgatact ttacttaaag aatgtgctga cttgatattt
      961 agcacatggc gagaagatgg acgtttcaaa ctatatccaa atggaggtat ttacccttgt
     1021 catactgcaa atgctgctaa cgtactctgt catatgggat atgcatctga cattagaatg
     1081 caaaaaacgt ttcaacattt tttagataca cagtatactg atggtgggtg gcgttgcaat
     1141 aaatttagtt ttggtcgagg tccagaaact gaatactcaa atccactacc aactcttaat
     1201 atattaaatg cattccgttt tagtaattat ctaaataaag aatcaaaact tgacaaagct
     1261 gtagattttc tacttgacca ttggactatt aaaaaaccac tcggcccttg tcactatgga
     1321 attggcacat tatttatgca agttgaatat cctttccgta attataacct attcctttat
     1381 gtctacgttt tatcatttta taatcgtgca aaaaaggata agagattttt agaagcattg
     1441 aagatacttg aatctaaaat gataggtaat aaaattgtcg ttgaacgtgt agttccaaaa
     1501 ctatctaaat tatctttttg taaaaaaggc gagtcaagtg aaatagccac aacacactat
     1561 tacgaaatat taaaaaacct agaaaaataa tagttatata gataaaacat attaaaaaat
//
LOCUS       DAMJVX010000324         1612 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.331, whole genome shotgun sequence.
ACCESSION   DAMJVX010000324 DAMJVX010000000
VERSION     DAMJVX010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.331"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(330..1037)
                     /locus_tag="PC407_003669"
     CDS             complement(330..1037)
                     /locus_tag="PC407_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2499053.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          510 a          279 c          141 g          682 t
ORIGIN      
        1 atttttaatc ttcatcaata ccttttttgc tttcattgtt aaaactattt tttctattag
       61 tttatttctt ataaaacaaa catttagtat taaaatatat atcacacaat agtatttgca
      121 atttaacttt aaaaaaataa aaatacccaa ctgacctaag caactcgatt gggtataaat
      181 aatctaaaaa atcatttttg tataaactct ttatttaact tacattttta tttaccttct
      241 actttaattc aacttatatt tctatgcttc ttatactctc attcaactta tatttctact
      301 tatcttatac ttttataaat tcatcacaat tatttagttc tcgctatcaa agaaattgta
      361 ttaaaatact ttccattctc tacttccatc atttttttgt catttatagc atatgaatta
      421 tcacaattta tccaatcctt ccaagcttca tcatgacaat ttaaagcttc acatttctct
      481 acaatcacag actccgattc agaccaaagt ttattccacc atgtaattga atggaagttc
      541 atattatctt gccaaaatgg aatgagttca tctggtatac aatccacaaa atcttctttt
      601 aatccaggca ttgccatagc aagtattccc ccctctttta caagaggtga tatatgattg
      661 tctaaaaatc cttctttgtt tccaaaatag tgataagaat cgatacttat caccgcatca
      721 aagaaacctt ctgcataagg tagttcatgt gcctctgcct gtattggaaa tattttatca
      781 tccaatttaa actctttaaa tcttttatag ttttcagttg gttctatcca taaatctgtt
      841 gcaaatacag tagcatcaaa ttcctttgct aaaaatatcg aagatatacc cttaccacaa
      901 cctaagtcta aaatacgcat tcccttttct agttttatct tacttgttaa ttcttccaat
      961 atttttatac aattaggtcc catcatattt ttcatgagaa agtccttatc gtatttattg
     1021 ctttttgtaa atatcattct atctttcctt tccctatcag ttattattta ttaaatttta
     1081 tatgtttgat tttttatatg cttcttatat tgagcaatct cttttttatt cattctttaa
     1141 cagacaattt taatgtgtaa ttttatccac tctgtgatag taactttaat gaataatctt
     1201 atctaatatg tgataattaa ctttgatggg taattttatc tactctacaa taatcacctt
     1261 aatgagtatc ttatctactc tattatagtt attttagtga gtaattttat ctactccaca
     1321 gtaatcgctt taatgaataa tcttatccac tctattatag ttattttagt gagtaatttt
     1381 atctactcta tagtaattgc tttaatgagt aatcttatcc actctattat agctattttt
     1441 atgggcaatt ttatctactc tataatagtc actttaatga gtaatcataa tttttatatc
     1501 attttagata cttactctaa taagaagtca ttttctaata cttaataagt tttaattact
     1561 tccactttaa cctatatact aattaactta tttaatttag tgcatatttg ac
//
LOCUS       DAMJVX010000325         1609 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.332, whole genome shotgun sequence.
ACCESSION   DAMJVX010000325 DAMJVX010000000
VERSION     DAMJVX010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.332"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            152..1555
                     /locus_tag="PC407_003670"
     CDS             152..1555
                     /locus_tag="PC407_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HDF2499054.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILIDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALVASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     DNGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKISDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
BASE COUNT          676 a          178 c          310 g          445 t
ORIGIN      
        1 agagaataaa aactttaggt atattgtttg agttgtatgg ataactatgg tatcatatat
       61 aaaatatggt aacaaaattg taacttttga ttaaaaataa gtatgagtac taataaaaaa
      121 tatgaaaagt acattggggg tagaaaataa tatgaagaat agtaaaaaga tattagctat
      181 aggacttaca ctatttttag taatggtaaa tactcctatg gtaaatgcgt tgacatcagt
      241 tgagcaaata aaagggaatg acagatatga gacagcagca aaaatagcag ataagcaaaa
      301 ctacaataca gcaatactaa tcaactcaga taatagctta gcagatggtc taagtgcaag
      361 tggtttagct ggagccttaa atgcgcctat tttaatgaca aagcaaaatc agattccaaa
      421 cacaactatg gaaagattaa acaaagcaaa gactgtgtat ataataggct cagaatcaac
      481 aataagtaaa aatgttgaga atcagttatt atccaaaaag aaggttgtac aaagaatatt
      541 tggtgaaaat agatttgata caagtataaa aatagctgaa aaaatcaaag aaattaagcc
      601 aatagataaa gtaattatag ccaatggatt tacaggagaa gcagatgcga taagtgcatc
      661 accagtagct gctagagatg gagtacctat aatacttata gatggaaata gtgtgggatt
      721 tgacacgaca ggcttaaaga gttatgcgct tggttctagt gaaataatca gcgacgaatt
      781 agttaagagc acaaattcta ttagattggg tggcacagat agatttgaaa ctaataagat
      841 agttatacaa gagttttata aaaatagtaa agaattttat ctaagtaaag gcttgcaatt
      901 aacagacgct ttggtcgcct ctacaatagc aaaaaatgct cctgtagttt tagttgaaaa
      961 tggcagtaac aagagtattt taagtggtgc agacaaattg actgtactag gaggcataaa
     1021 tcagaatgtt atcaagcaat gtataaatca agcttcacca aatcaacaag ggttatatta
     1081 caatccaaat gatagagcat ttaaagagag aataaagggt aaagtatatg ctcttacaaa
     1141 gcagtataga aaagataatg gagtaagagc gttatctgta gcaagcagat tagagggtct
     1201 agcaaatgat tggtctaatt taatggcaaa taagaagact ttatctcata cgataaatgg
     1261 aaaaaattca tattctactt ttttgaaata cttagattgg agtgagatta aacctggata
     1321 tatagcagtt caaggtgaaa atataattaa atataaaatt tcagataaac ctgtatatac
     1381 aaatagagat gcagatgata taggaaactt tatttttaat gagtggaaga caaatccaga
     1441 agaaggaact aatatgttgc ataaagggta tgaaataatg ggatttggta tagctataac
     1501 aggagataag aatctatatg ctacacatga attttatgga agatataaag aataagaaaa
     1561 tatacacaaa attatgcttg taaggaagct atataaataa ttaaattat
//
LOCUS       DAMJVX010000326         1546 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.333, whole genome shotgun sequence.
ACCESSION   DAMJVX010000326 DAMJVX010000000
VERSION     DAMJVX010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.333"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(32..382)
                     /locus_tag="PC407_003671"
     CDS             complement(32..382)
                     /locus_tag="PC407_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499055.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKTVTSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            complement(605..796)
                     /locus_tag="PC407_003672"
     CDS             complement(605..796)
                     /locus_tag="PC407_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499056.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            complement(915..1463)
                     /locus_tag="PC407_003673"
     CDS             complement(915..1463)
                     /locus_tag="PC407_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2499057.1"
                     /translation="MGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKADIEPVL
                     LKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEERLSEFI
                     NGNTLKYDFVKMPHHGRYDKLTETFLESTSPQYAVITCSEKKEPEEGVLKILERLNIK
                     TFLTSSGEVVINSDGKTLSVNQ"
BASE COUNT          505 a          277 c          152 g          612 t
ORIGIN      
        1 attaatttat ttcaatattt tcatatatct tttattctaa attcaatact tttctaatat
       61 cttctatacc ttgataaact tcttgtttag aaaaattcat agcttctaat tcttcatcag
      121 tcttttccat aagcatagta taaaattcta ttgctatata ctgcatattt tcactagtta
      181 cagtcttagc tttatcaaat aaaatttctt ctgcttcgtt tacatttccc tgaaaaacta
      241 atctttttaa agttataagt ataacttctt cttctgtatt atacactaaa tctttttctt
      301 catattcact gtattttttt cccaataaaa gtcttgcaac cataagtttt atctcattga
      361 aaatcttatc cactatagcc atttttacat ctcctataaa taaattttat tttcttttta
      421 attatatcag actttattgt aaaaattatt atatgtaaat gaaataattc aaaaataaat
      481 tctaacaata aaaaaaagaa ggtagcgggg gatttccgac taccttcaaa cactagattg
      541 tgattggttt ggtattttat ttatacccac cataagcttt attaaatcat ctaaattgat
      601 ttttttagca tccacatcct ccatcaaaaa cgtcaccaca ttcacaaagt attaataata
      661 atattaagaa aggtatccat gcacataagt ccatttcagc ccaacaatct tggaatgcta
      721 agaataagaa gaataatatt attatccacc aagcacatcc accaaatccg ccttcaccaa
      781 aaaatctatc caacataata tgttacctcc tagtcatttt gtatgtggag tttcagctcc
      841 taaaatatac tatgtttaag gggtaatatt tgctaatgat ttttttaaat tttttttaga
      901 tattttaaaa aaaattattg attcacactc aatgtcttac catcactatt gataactacc
      961 tctccactag aagtcaaaaa tgtttttata ttcaatcttt ccaatatttt taaaacacct
     1021 tcctctggtt cttttttctc tgaacatgta ataacagcat attggggtga agtagactct
     1081 aaaaatgttt cagttaattt gtcatatctt ccatgatgag gcattttaac aaaatcatat
     1141 tttaaagtat ttccatttat aaactctgac aatctttctt cctctgcatc tcctgcaaat
     1201 aaaaacttat gtgctccata gcttatatta gtgattattg aataatcatt tgaactctcg
     1261 tacttgcttt ttgaagccgg atgtatgttt atttctgcac tattgattac aatattcata
     1321 ttatctttta gtagcactgg ttctatatca gcttttttaa gtgagtcaat atattcatca
     1381 tattgcttac tatctttctt ataatttgct tgtataatat tctttacttt aacactagat
     1441 aacacactat ctactccacc tatatggtcc ttatctaaat gggttaaact gaacctccta
     1501 tgactaaagt cacagggttc ctgcttcata gaattttgca tactaa
//
LOCUS       DAMJVX010000327         1530 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.334, whole genome shotgun sequence.
ACCESSION   DAMJVX010000327 DAMJVX010000000
VERSION     DAMJVX010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.334"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            24..1460
                     /locus_tag="PC407_003674"
     CDS             24..1460
                     /locus_tag="PC407_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HDF2499058.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPIVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT          628 a          160 c          265 g          477 t
ORIGIN      
        1 attatgaggg ggacaatttt attatgaaat tctataaaag aatattaaca ttgactttaa
       61 gtatttcttt tgtatttgca aatattcaat tagtagatgc aatatcatct gtagaaaaaa
      121 tacaaggaaa aaataagtat gaaatagcag gcaaaatagc agataaaaat gcatataaaa
      181 ctgctatatt aattaatact agtaactcta ttgcagatgg attaagtgca agtggactag
      241 caggagcttt aaatgcccct atactattaa ctgaaaaaaa tactatacca actgagacat
      301 ctgcaagatt aaaaaatgta tcaaaagttt atataattgg aggaacatat tctattagta
      361 catctgtcga aaattcatta aaatctaaga aaatgaaagt tgtaagaatt aagggaaatg
      421 acagaataaa aacaagttat aatgttgcaa aagaaataaa ttcaataaaa aaagtcaata
      481 ctgttatgct aaccaatgca tataaaggag aagcagatgc tataagtatt gcatctgtag
      541 cagcaagaga taaagcacca ataattctta ctaatggaca aagtatacct ttttcaacta
      601 gtggtttaaa gtcttatgta ataggaggaa cagcttcaat gagtaccact ttagtaaaca
      661 atactaaatc tactagacta ggagggagta ctcgttttga aactaataaa gcaataataa
      721 ataagtttta taaggatgct agagagtttt atatagctgg agcttatgaa ttaacaaatg
      781 cacttgtagg atcttcttta tcaaaacatg gtccaatagt tttagttaat gatggtagca
      841 ataaaagcat tttaaaaaat gctaagaaga taacttctat aggatatata gattcaaata
      901 tagtacaaca gtgtttaaat ataacaaatg gaattggaga tattaatact ggggttgtaa
      961 aaaatgttaa acctactaca aagactataa aagatggaat gtataaggtt ggaaaagata
     1021 tatcagcagg tgaatactta attacttcaa actcaggaag ttatgcttct tattatgaag
     1081 ttacaagtga tagtacaggt aatgctgata gtatactttc aaatgatatt tttagtggga
     1141 ctagatatat caccttaaag aatggtcaat atattaaaat tgaggattca acaatgacat
     1201 tagcaaagta tgctaaagcc cagaaggcca agaatggtaa gtttggaaat ggtatgtata
     1261 aaattggatt agaaattcct gctggagaat atattataat gtcaaattct tctgatgcat
     1321 attatgaagt tagaaatgat agcttaggta atgcagaggg aatagttact aatgatactt
     1381 tttcaggaag aagatatata acggttgagg aaggtcaata tttaatatta aatgattgtt
     1441 atttaattga aaatgaatag atgtaattgt atttaatagt aaatcatata ttttatttaa
     1501 ggaaagatgt tttaagtttt aatttaaaac
//
LOCUS       DAMJVX010000328         1447 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.339, whole genome shotgun sequence.
ACCESSION   DAMJVX010000328 DAMJVX010000000
VERSION     DAMJVX010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.339"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            59..1297
                     /locus_tag="PC407_003675"
     CDS             59..1297
                     /locus_tag="PC407_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2499059.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKRNKSATKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT          616 a          134 c          251 g          446 t
ORIGIN      
        1 tatataaaaa tacttatcta gttactagca attattagta gaaatggggg aaatagagat
       61 gaataaggga ttttacaaat taaattcagt ggtattgaac tttgatatta agaatgatgt
      121 taacaagata agatttaatc attatataat gagaaaagaa aacatacaaa gacttcaagg
      181 aactctgcca aacggacaat tttatatgac agtgagaaga gctgctacag atttacaact
      241 ttctatttct actataagta ggcttgtaaa agagtttttg gatttaggga taataagatt
      301 agtgtctaag ggtgtaaaag gtaatcgttc tgtatatagt tacgtaagta tagagtttaa
      361 tggaagttta tttgatgata gcaaatatat ggaacttgac tgtgtatgta atgatgaatt
      421 tattgataat agtggcatag agtttgattg tgatagcaaa gataattctt ttaatggaga
      481 tataaatgta gaaactgtgc attgcattaa tgagtatttc tctgatgatg atgtagagca
      541 taattataac aatttccata ataaagatac tataaaggat aaagccattt ctaatattag
      601 cactatcaaa aaatatattg atgaatcttt tgatacaaat aaatcagaaa attctaactt
      661 acaacaggag tcaataaatt tcaactttaa tgatacatgc aatgaaatag ataaaagaac
      721 gaataatagt atagaaaata atcaaaataa aaggcctatg agattgagtg acatcagtgt
      781 tttaagtgat aaaggtatga caaataaaaa gaattacttt aatcataatc atggtataca
      841 aaaaaggaat aaatctgcaa caaagaaaaa agaatcctta aataaattta ataaaaaaaa
      901 tatacagcaa agcattataa atatgctaaa taaaaaatca ggtaagaatt ttaaaatcga
      961 ttctccaata agcataaaac ttatcaatga aaggctaaag gagggctatg tcttagaaga
     1021 tttctataaa gttattgagg taaaagtggc taagtggaaa aataccataa tggaaatgta
     1081 tataagacca gaaaccttgt ttagctataa gtttgaatcc tatgtaaatg aaaacatatc
     1141 tatgaaaagt caaagtaatg ttcatccatg taaagaaggc aatagtaatg atagcagtaa
     1201 ttatatacat tctgattata caacatatga tggcagtgtg cgtgagagaa aaaaatatga
     1261 gaatgtaaga tactggaatc ctatatgtga aatttaatta gtttaagaat gtatttatga
     1321 taaataagat attaatagag aatagtgagc tatagcaaat gatatatcaa tagttggtag
     1381 taagttatat taagcaagat attactatca ggtagtgaat tatagtgaac gatatatcaa
     1441 taattgg
//
LOCUS       DAMJVX010000329         1422 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.340, whole genome shotgun sequence.
ACCESSION   DAMJVX010000329 DAMJVX010000000
VERSION     DAMJVX010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.340"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(147..1007)
                     /locus_tag="PC407_003676"
     CDS             complement(147..1007)
                     /locus_tag="PC407_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-116"
                     /protein_id="HDF2499060.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1181..1390)
                     /locus_tag="PC407_003677"
                     /pseudo
     CDS             complement(1181..1390)
                     /locus_tag="PC407_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          372 a          341 c          336 g          373 t
ORIGIN      
        1 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttaaa tatttgctta
       61 tacaatcttc ctgtttttgg ggcttttctg attatcaacc ggggtaaatc aatctaaagt
      121 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
      181 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      241 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      301 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      361 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
      421 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
      481 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
      541 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
      601 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
      661 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
      721 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
      781 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
      841 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
      901 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
      961 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1021 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1081 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1141 gtagttaaca aaaaaaagcc cgccgaagcg ggctttatta ccaagcgaag cgccattcgc
     1201 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     1261 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     1321 agtcacgacg ttgtaaaacg acggccagtc cgtaatacga ctcacttaag gccttgacta
     1381 gagggtcgat cgcggaccgc tggcggcacg gcgcagggac gc
//
LOCUS       DAMJVX010000330         1393 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.341, whole genome shotgun sequence.
ACCESSION   DAMJVX010000330 DAMJVX010000000
VERSION     DAMJVX010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.341"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            72..353
                     /locus_tag="PC407_003678"
     CDS             72..353
                     /locus_tag="PC407_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499061.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            617..871
                     /locus_tag="PC407_003679"
     CDS             617..871
                     /locus_tag="PC407_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499062.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            916..1239
                     /locus_tag="PC407_003680"
     CDS             916..1239
                     /locus_tag="PC407_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499063.1"
                     /translation="MATEIFTVKITPLEAQKMIKENIDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTEKTVIKATSTGSAIGVLKIDWGAGGNLLKRVKNIL
                     AEYII"
BASE COUNT          586 a          104 c          221 g          482 t
ORIGIN      
        1 atacatataa agatattttt aagaataaaa tttattatta aataaaaaat aaaggagagt
       61 gatttaatcg tatggcatca aaacaaacac caagaaacaa tttagaaaag tttttaagaa
      121 taagagtctt atcacataca ggcgataaaa tacatataaa gttacctgta aattttgcaa
      181 aaagaatgat tgagaataat gctttggatt tatttaatgg aaaagatgat gtagtagatg
      241 gtaaaaaggt tactgaaata ttagtcaaag catttgacta tgatttaact ggagaagtag
      301 ttcacttgga gagaaaaaat ggagatatta ttaaagtttc aattgtacaa tagaattatt
      361 ttatatgaat tttatttttg aatagttata aataattaga aatttgtaaa aaagtataat
      421 atattattag taatggtgca gaatacacat ttataattta tagatgtgta ttttttgttt
      481 aaaaaattta aatatataaa actataaagg ttacaatttt ggaaatagat gtaaatatat
      541 gttctattgt ataataagag taatataaaa tctagtaaaa ataatcaaat aaatttaact
      601 tatgagaggg ataactatgg aaaataagga ttttaaatta acaatatgta tatttgcttt
      661 tgtaattgga gtagtagtat tattctgctg tataggtttg ggtgaattta gtgcagagca
      721 tattattcaa caaaatggtg gaatcatgga tactgaccaa tataatattt atctagacca
      781 atctatttct caatatagaa attttggttc tatattagct ctgttaggag gaatagggat
      841 attgtttgat aaatatatca gaaaatacta gagattatgt tttgtctaaa ataaattctt
      901 atgaggagat aaaaaatggc aacagaaata tttactgtaa aaattacacc cttagaagca
      961 caaaaaatga taaaagaaaa tattgatgca gatttagttt ttagtgattc ttataattta
     1021 ggagatgata aatttatact aattacaact tttgagaagt attacgcaag aaataatagt
     1081 gatgcaggat taatcgtagt ttatgaaaat accacagaaa aaacagttat taaagctact
     1141 tcaacaggca gtgcaattgg agttttaaaa attgattggg gtgcaggtgg aaatttactt
     1201 aaaagagtaa aaaacatact tgctgaatat attatttaat tattttatct aactgataat
     1261 atgtcaagaa aatctcgtaa aagaggttaa gaatattttt ataatatata gataaataag
     1321 gatttgaagg taaagttttt aatgtataaa gaaaaataat ataataaaac gaattggctt
     1381 tataattctt tat
//
LOCUS       DAMJVX010000331         1378 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.342, whole genome shotgun sequence.
ACCESSION   DAMJVX010000331 DAMJVX010000000
VERSION     DAMJVX010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.342"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(625..1218)
                     /locus_tag="PC407_003681"
     CDS             complement(625..1218)
                     /locus_tag="PC407_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2499064.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT          495 a          193 c          180 g          510 t
ORIGIN      
        1 ctaatctaaa gaagtataat aaactaattt aggaagtata gaaaattaat ctaaagaagt
       61 ataataaact aatttaagaa atatagaaaa ttaatttaaa gaaatacaat acattaattt
      121 ttaagtaaca ttaaattaat gtaagtaaaa taatgttcat atgaagattt aaaggtgcat
      181 tttctgaagc taagtatatg ttatgtctct atatccggtg aagtttaata gctcaagaaa
      241 atgcactatt tatgatgatt attttgtttt atttccaatt ttggaaataa ctattctaat
      301 taaatctaaa attataccat aaataagaaa agaaagtata gatgctatgt accaataaat
      361 cgaaattgac cctgtaattg ttctacatgg gatttgcatg cctgaatcca taaaaacaaa
      421 aaaactatca atggtagaaa taatagacaa aataggattt aatcctatta gaataatact
      481 ttttgagtct tgaccaattt ggtggatata aatctcaaat aatgcaatta aaaaaaacaa
      541 aatgtaaaag atttaataat ttttttcatc aaaatacctc acctaatata aattctgttt
      601 gcactatttg cttactataa ctatttatct ccattcagtc acatcactta attgtaatct
      661 aggtcttctt gatggttctt cgtcaggata acctaaagat attgctgcca ctaattgact
      721 atctgtattt aaccattcac ataattcacg atatgcaaag tatacatcac aaatccaaag
      781 actacctaga cctaattcca cagctgtaag cgacatattc tgaattgcag ctccaattga
      841 ttgcatgttt gccatttcat agaatcgttc ttcaggggat agcttttcta aaggtgattt
      901 acctaatata tttaaaataa aaattgttac tggagcttgt ttcatgattt caactgtgta
      961 atttgcacca gcgatatgtt gacgacttcc tggaagaagc ccattatcat taatttcatt
     1021 ttgaatacct ttgctcatgg ctttaagcat agattctttt tccttttcag tgatgacaac
     1081 aaaacgccat ggttgcctgt ttttagatga aggtgcattt attccagctt caattatttt
     1141 ctctatagtt tcatgtgaaa tgctttgatt tttatatttt ctgatacttc gtctttttga
     1201 tatagaatca cttatcattt taagtaacct ccgtttattt tttggaaaaa tctatttatt
     1261 ttctattttt aattatttta gcattaatca gaaaaaaagt cattgatttt agtatacaat
     1321 tattaacgat tatattaagg attgtatcaa aaagtatgtg attatactaa gggattat
//
LOCUS       DAMJVX010000332         1368 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.343, whole genome shotgun sequence.
ACCESSION   DAMJVX010000332 DAMJVX010000000
VERSION     DAMJVX010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.343"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            94..903
                     /locus_tag="PC407_003682"
     CDS             94..903
                     /locus_tag="PC407_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2499065.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKEKL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEV
                     QNNKRPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            946..1212
                     /locus_tag="PC407_003683"
     CDS             946..1212
                     /locus_tag="PC407_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2499066.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTDIEKPFWGEQCPVKSCCED
                     KNLIHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
BASE COUNT          540 a          155 c          196 g          477 t
ORIGIN      
        1 tcttattaga atatttcaat taaaacaaaa ctggtttatt atataaaaaa taaattagta
       61 tacttatttt ttatatatca ggaggatata cttatgaata aaataatcaa taacatttta
      121 aatacctgca aacagtatac atatattttg ccacataaat tgttaagaca gtatatagca
      181 cactatacaa tatctattcc agatacaagt attaaagaaa agcttacatt aatacctgat
      241 gctagtggat gtatgatatt taaatttgac aaaaaaggta tagaaagcgc attctgggga
      301 gccacaacaa aaactactat tgtaaaaaat gatatagaaa atgtactttt tagagtattc
      361 gttgagtttc gcccaggtgg agtatattat ttgacaggtc tttctcaaag agaatctaca
      421 gatttaaaaa tttcacttga agattttaac acattattta gcttagaggt taattccatt
      481 tttgaaagaa caagtactat aaaggaatta gtagaacaat tagatatgtt attcttatca
      541 tacttattga aatcaaatat agtagatatg actataccaa ttcttgaaaa tgctagaaaa
      601 cagaactcta ttatgtctgt taaaaacatt tcacaaatta gttgttatag tgaacgacat
      661 ttaaatagaa tttttaataa ttctcttggg atgagtgtta aatcatacct tagattactt
      721 cgtataaacc ttgtattaca agaagtacaa aacaataaaa gaccatttgc aactttagca
      781 caagatattg gttattacga ccaatctcat tttataaatg attttaaatc tatttgtggt
      841 gttaatccaa caacctatat aaaaaatttg tccgattttt acaatgaaaa gtataaattt
      901 tgatatacaa tcataataaa atttatattt aaggagtgac ttaaaatgat agaatcaaga
      961 tgtggaattt tatgttatga atgtggatac aaggaaaaag tcaattgtaa aggttgtaca
     1021 gatattgaga aaccattttg gggtgaacaa tgtcctgtaa aatcttgttg tgaagataaa
     1081 aaccttatac attgtggtct atgtgataca tttccatgtg aaatgcttaa tcaatttgca
     1141 tatgacaaag aacaaggtga tgatggcaaa agaatttctc agtgcaaaaa atgggctgta
     1201 ctagccttat aatatactct cttgttataa aataagtttt tgtaaggtaa attttttaaa
     1261 ctgttgtaga aatttctgca acagttttta taattaaatt taaaggttat ttaattaaat
     1321 taatagaata gtaatttatt gataaaatct gattactaaa attagaaa
//
LOCUS       DAMJVX010000333         1349 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.344, whole genome shotgun sequence.
ACCESSION   DAMJVX010000333 DAMJVX010000000
VERSION     DAMJVX010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.344"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            361..738
                     /locus_tag="PC407_003684"
     CDS             361..738
                     /locus_tag="PC407_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2499067.1"
                     /translation="MKEEILIEKLLRAELIVMEYLCKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINFQYHYKIIIDNKEYYTFKKKVLKSSKSIKIMRSLT
                     TTHKNVSKEKLDLLDEYYRNLKE"
     gene            843..1218
                     /locus_tag="PC407_003685"
                     /pseudo
     CDS             843..1218
                     /locus_tag="PC407_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT          608 a          113 c          178 g          450 t
ORIGIN      
        1 atatatatta ttacatacct aaaaaacttt cataaaaaca aaaataaaat atgcttttag
       61 taacttgtta acataactta taatttattt tataagcatt agcctatata gttttgtgct
      121 actaaatttc tttgattcta acgatttagt gctattctac aactaaaata atacaataga
      181 gctatttatt gtttggtagc attgtttgca aattaaacaa atatatatat atgaatatta
      241 ttttacgttt aaaaaaacag actaaatatg tagaatattt ggtaaaactt acctctagtt
      301 tttgtcttaa aaagctccta ttattaattt aagttagaag aattaaggaa gtgacataaa
      361 ttgaaagaag aaatattaat agaaaaatta ttaagagcag aattaatagt aatggagtat
      421 ttatgtaaaa aagacactct aatgcctaaa aaagaaattg taaaagaaat aaaacagatt
      481 tatggatggc ataaaagtac aataaaaata ctactaaaaa ggctagttgc taaaaggtat
      541 ttggctagat atattataaa ttttcaatat cattataaaa taataataga taataaagaa
      601 tattatactt ttaagaaaaa ggtactaaaa tctagtaaaa gcataaaaat aatgcgttca
      661 cttaccacta cacataaaaa tgtaagtaaa gagaagttag acctattaga tgagtattat
      721 agaaatttaa aagaatgata tagtaaaaat cgaatgtaat tatttacctt aattcttttg
      781 tttgtaaaac tttattgata tagaatttaa catatacata aattggtaaa gggatgtata
      841 ctatgaaaat tagtaaatta ccagaagcgg aattaaaagt tatgagatat atttgggaat
      901 cagaaaaggt attaatatca agagaaatca ccaagtctat ggaacaaaaa tatgaatgga
      961 aagatacaac tatatttaca gtattaaaaa gattactaaa gaagagagtt tttagaaacg
     1021 gagaaaatag ataagcgtac acactataat gtattagtaa aagaaaaata atatcagaga
     1081 ttttaaacaa aagaattttt aaagaataca cataaaaatt caatattaag tttaatgtca
     1141 gcactatgta gtaaggatga tgaagtagat ggtgatattt taaatttatt agaagaatat
     1201 tttaagaatt taaaataaga aaatgacagc tagatacata ataaataaaa tgaaggtatg
     1261 aaattataac tttttctaca ttcattttgt ttattattct gaacatgtta aaaaaatgaa
     1321 ataagtttgt attttattta tataaattt
//
LOCUS       DAMJVX010000334         1329 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.345, whole genome shotgun sequence.
ACCESSION   DAMJVX010000334 DAMJVX010000000
VERSION     DAMJVX010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.345"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            206..1303
                     /gene="ychF"
                     /locus_tag="PC407_003686"
     CDS             206..1303
                     /gene="ychF"
                     /locus_tag="PC407_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDF2499068.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT          511 a          148 c          243 g          427 t
ORIGIN      
        1 tattttctaa aagcaatatt tttatttaaa ttaaatgcaa atattattta aatcaatatg
       61 ctttaaagtc tagtttacaa gcttgatata tatgatgtat aatcaatatg tatgtctata
      121 attaaaacaa ttttgcaatc ctatagattg attgtttaaa gttatataaa gaatataaaa
      181 aagaataaga gagaggacga ttaatatgaa attaggtata gttggtttgc caaatgtagg
      241 taaaagcaca ctatttaacg caataactca agcaggtgca gaatctgcaa attacccttt
      301 ttgtacaata gacccaaatg tgggagttgt atctgttcca gatgaaagat taaacaaact
      361 acaagaatta tataattctg aaaaaatagt tcctactgct atagaatttt gtgatatagc
      421 aggtttagtt agaggtgctt ctaaaggaga aggtttagga aataaattct tatcacatat
      481 aagagaagtt gatgcaatag ttcatgttgt tagatgtttt gaggatgaaa atgttgtaca
      541 tgtagatggc tctgtagacc cacttcgcga tatagaaact ataaacttag aattaatttt
      601 ctcagatata gagattcttg aaagaagaat taataaaact caaaaagctg ctaaggctga
      661 taaaacttta ggttctgaac ttgatttatt aaaatcaata atgtctactt tagaagaaag
      721 taagtgtgta agaactatgg aatttacaga agatgaacaa acattcgtaa attcattgga
      781 tttacttaca tctaagcctg taatatatgc ttcaaatgta tcagaagatg atttagctga
      841 taatggagaa aataataaat atgttcaaca agttaaagca tttgcagaaa cagaagatgc
      901 cgaagttgtt gtagtatgtg cacaaataga agcagaaatt tctgaacttg attctgctga
      961 agaaaagaaa gagtttttag aaacattagg tcttgaacaa tcagggcttg ataagcttat
     1021 aaaatcttct tatgctttac ttggtttaat ttcattctta actgctggtc caaaagaagt
     1081 tagagcatgg actataaaag ttggaagtaa agctcctcag gcaggaggaa aaatacattc
     1141 tgatatagaa agaggattta tcagagcaga aacaatagcc tttaatgatt tggttgagca
     1201 tggtactatg gctgctgcta aagaaaaagg gttggttaaa ttagaaggta aagaatacat
     1261 agttaaagat ggagatgtaa tattatttag atttaatgtt taaaatgatt aatcatatac
     1321 actttgact
//
LOCUS       DAMJVX010000335         1312 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.346, whole genome shotgun sequence.
ACCESSION   DAMJVX010000335 DAMJVX010000000
VERSION     DAMJVX010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.346"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(19..1095)
                     /locus_tag="PC407_003687"
     CDS             complement(19..1095)
                     /locus_tag="PC407_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2499069.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
BASE COUNT          385 a          227 c          178 g          522 t
ORIGIN      
        1 atacatattt ttacaatatt atttattaaa acctaatata tcatcaatat tatctaataa
       61 ttcttgtaca tcttcgacag tatagtcacc catatgtgaa attctaaaag ttttttcttt
      121 taaatctcca tagccatttg ctatttggaa tcctcgttct tgtaattttg aatttaaatt
      181 tgatacacta ataccttgag tattttctat aacagtaagt gtattggata agtgattttc
      241 attggtaaaa atttggaagt gttttttagc ccagtttcta actaagtttg ccatgtcttc
      301 atgtctgttg aatctgttat ctaggccttc atctaatata ttatctaatt ggaaatctaa
      361 agcgaacatg tgagaaagag atggtgttga agggtattgg tagtttttct tgattaagta
      421 ttcatacatt gctaacaaat caagatatac tcctctaaaa ggtacttgct tagctctatc
      481 gattgctttt tgagaaaatg tgcaaataga cattcctggt ggtaatccca gtgctttttg
      541 tactgatgtt atacaaatat ctattccaat cttatcaacc tcgactttta ttccaccggc
      601 tgaacttaca gtatctacac agtagattac atcttcatac tttttaacaa cttctccaat
      661 ttcttctatt ggatttctta ttccagttga agtttcattg tgagttacag ttattaaatc
      721 gtattctccg gtagaaagca ccttatcaac catttctggc gttgttgcct ctcctaattc
      781 aactttaaat atatccgctg ggacattatt agccactgcc attttgtacc atctatcacc
      841 aaaggatcca caggaaaata ctgctgcctt tttggatgta caggaacgaa tggagccttc
      901 cattaaacca gttccggatg acgtggataa aagtatcgtg ttgtttgtat agaatagttt
      961 ttgcatcttt tcacttatac tcttttggag catagatgca tcttttcctc tatgtcctat
     1021 cattggtgta gccatctttt gcaagacttc ttctttaaca tcaataggac caggaataaa
     1081 taatttctta ctcattataa ttcccccctt tttagtttgt aatatggata gcgtttttac
     1141 tgtccttatt tttaataact ataatatcaa gctgaattaa ctatgtcaac ataaaattta
     1201 ttttttatat ataatttatt ttatatattt tatgaaaaca ttcattttct gccttttcat
     1261 aagtctgaat tttctgaatt attctctcaa aaaaaatata ctttttatct at
//
LOCUS       DAMJVX010000336         1308 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.347, whole genome shotgun sequence.
ACCESSION   DAMJVX010000336 DAMJVX010000000
VERSION     DAMJVX010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.347"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            126..1166
                     /locus_tag="PC407_003688"
     CDS             126..1166
                     /locus_tag="PC407_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2499070.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
BASE COUNT          531 a          130 c          241 g          406 t
ORIGIN      
        1 ctttttatta agttttgttt taaaataata ttattaaatt ataactaagc ttttctgtta
       61 tgatataatt aaatagataa tgtgaatgat tattatatag aatacttatt tggagttgat
      121 attttatgga caaacacagt gattcagtaa tgtatggtgg aatggaagta tataatattt
      181 ggggaacaac aacagtagac tccataattg gagagtctga tgtaatgcgt gctcttaaga
      241 atagaatttg caaaattgga aatagtgatt ctactgtagc tataactgga gaaagtggca
      301 ctggaaaaga attaatagca agagccattc atctcgcagg aaacagaggg aataaaacat
      361 tcgttgctat aaactgtgct gctataccag aacctttact agaaagtgaa ctttttggat
      421 atacaaaagg agcttttagt ggagcagatt caaaaggtaa aattggtaag tttgaattgg
      481 ccaatggagg ggttgtattt ttagatgaga taggagatat gccaattcaa ctacaatcaa
      541 agttattgag ggtattacag gaaaagaagt ttacgagaat aggttcaaat gaactaattg
      601 atatagatgt tagaatcata tcagctacta atagagattt atttgagttg gttaaggaaa
      661 ataaatttag agaagactta tactatagat tgaatgtaat acctttagaa gtgcctccac
      721 ttagagaaag aggagacgat atacaaatat taataaataa ttttatagac aaatattcga
      781 gaaaaatgaa gaaaaacgtg taccatatag aaccagatgt ggaacaaatg ttgcttaagt
      841 atccttggcc aggaaatatt agagagttgg aaaatactat agaactggca ttaaaccttc
      901 ttgaggaaga tggaataata cataaaggaa taataaatag aaagatactt gaatatttta
      961 agttaaataa tataaatata aagttacttg aggaaagtga ctatgagata agtatggaac
     1021 aagacatact tacacttgaa gagtttgaaa gagcctatat aaagaaagtg ttaaagtttt
     1081 acggaaatga tactaagtct aaaaaactgg ctgccaaaaa acttggaata tcactagcta
     1141 cattgtacag aaaaatagat gtataataat taggtttttt attctcaatt ctaaaatttg
     1201 aaattataaa attgataaaa aacacttgga aaactatttt ctaagtgttt ttttgtgtaa
     1261 tagcatttta ttatccaaaa ataaaaaaaa tttaaaagaa tttaaaaa
//
LOCUS       DAMJVX010000337         1293 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.348, whole genome shotgun sequence.
ACCESSION   DAMJVX010000337 DAMJVX010000000
VERSION     DAMJVX010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.348"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            124..501
                     /locus_tag="PC407_003689"
     CDS             124..501
                     /locus_tag="PC407_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2499071.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
     gene            complement(930..1193)
                     /locus_tag="PC407_003690"
     CDS             complement(930..1193)
                     /locus_tag="PC407_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499072.1"
                     /translation="MIKNTKQITVEVNEILLQEISKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
BASE COUNT          491 a          110 c          170 g          522 t
ORIGIN      
        1 taaaaatata aattttttgt attaataaat ttggatgaga tattttaaat aaaacctttg
       61 actcgaaaag tctattaata tagtatttaa ttatgtagaa gtttgagagg agtgtaaaat
      121 catatgacta ttactaagat accaccagca gaattaaaag tcatgaaatt tatttggagt
      181 tcagatttta cagtagcatc aaaagatgtc attgaagcta tggaaaaact atataattgg
      241 aagcaaacta caacactaac acttttatca agattagtaa ataagcaatt cttaagtgca
      301 agaaagattg atagacatac acattataca gttcttattg gagaagaaga gtatttacat
      361 tttgaaacaa aaactttttt agataatatg catggaagtt ctattgagag ttttatgaaa
      421 tccttgttca aaaagggggt aaatgaagaa gaattgaact ctgttgaaaa atgggttaaa
      481 gatattaaaa aagatgaata atttggtgta gttaattatc ataaaataaa tagtttttac
      541 aataattagt taaatgaata aaatataaag ttttatgtaa aatttgtaat ttttataaat
      601 attttgtctt gttatttaat attaatacaa taagtttgat gtaaaatcaa acctgtttga
      661 atttaattta tattgggttt gtcattagta ttattttagt ttgatattgt atacgaattt
      721 aaaagtcaga atttttgtat attaatcatg taaattaatt gttagatata aagggcataa
      781 tttttgtttt gattatttct agggtaagat aagtatacaa tagattgtat taaacatgct
      841 tatattgata aatttgagat aaatattgag atatattaat aattattaat ataaaaaagt
      901 tcctagtttt taggaacttt tttatttgat taatacaggg tatgagtttt tgcttcagct
      961 atagctcttg ctgcactttt aggtttttca gtattttctt ttttgtaaaa tttaatcact
     1021 tcttctaaat ctttattttc aatgttatgt tttttgtttt caagaatata atttacaatt
     1081 gattcattta tcaaatctgt caaaatatta ttttcgtcat tagttatctt agatatttct
     1141 tgtaataaaa tttcattaac ttctactgta atttgttttg tattttttat catatcaacc
     1201 acctcttaag aaaattatat catctcaaaa aaataaattc aatatgtttt aaaattatag
     1261 aattttaatt cttaaattct aaaaaagaga aaa
//
LOCUS       DAMJVX010000338         1276 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.349, whole genome shotgun sequence.
ACCESSION   DAMJVX010000338 DAMJVX010000000
VERSION     DAMJVX010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.349"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            214..924
                     /gene="rgaR"
                     /locus_tag="PC407_003691"
     CDS             214..924
                     /gene="rgaR"
                     /locus_tag="PC407_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HDF2499073.1"
                     /translation="MFRVVICDDEKIQRSILKEFTKKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          507 a          104 c          193 g          472 t
ORIGIN      
        1 ttatatttat tttactatat ttttttacat aatatatcta gaaaagtata ggaatttatc
       61 gtaatttaag tttaaatatt aaagagaatt tctataagta gtttaatcga caacttaaat
      121 ttataaagaa atttaaattt gaaagctagt gagtacagtt tggaaattga aataaagtaa
      181 actaaatgat attttagatt tggaggtgat attatgttca gggtagttat atgtgatgac
      241 gaaaaaatac aaagaagcat actaaaagag tttacaaaaa aacttttgtc tgaaagatgt
      301 ctaaattatc aaatactgga attttcctgt ggagaagatt taatatccaa atacccagaa
      361 aaaattgata ttatattttt agatatccaa atgaaagata tcaatggaat tgaggtggct
      421 aaaaaaataa gaaagtttga tgaaaaagta gaaattatat ttacaacagc tttttcagag
      481 tatgcccccc aaggttatga ggttcgtgct tatagatatt tagtaaagcc tatagagtat
      541 agtaactttg caatgggtgt aaatctttgt attgataacc ttcaaagaaa gaaggagcgt
      601 tatatagttt taaatagtaa aaaaggattt aatagaatac tgatagattc tattttatat
      661 gttgaaactg taaagagaga tttagttgta catacaatag gtagagatta taaagctaat
      721 atgagcatga aacaagcaga aaaattactg tgtgaaaatg gcttttttag atgtcataca
      781 agttttattg ttaatttaag aagaatagaa gatcttaggg ataatatgat tactattaat
      841 gaacaattta tacctataag taagtataga cttaaagatt taaaagtagc attaacaagc
      901 ttgttatgtg atataatgta ttaattttaa aaaggtaatt gaaaatatcg taaaaaagat
      961 ttaatgtcat cacagggcta ttgattaaaa ctgtcttaat atataaaatg ttatttatat
     1021 taagacagtt ttattatgca aaatatcagt ttatagactt ttaatattgt ttaatatgta
     1081 tatgtagagt ttccattaca gtagcatgat tattaattag tagcctgatt attaatatac
     1141 tattattgat ttttatatgc attatatgta tttttagaat atgattacct ttttaaaata
     1201 atgactattt ataaaactct atagatattt gtttcattta gctatgcata ctttaattaa
     1261 ttatattaat atttaa
//
LOCUS       DAMJVX010000339         1253 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.350, whole genome shotgun sequence.
ACCESSION   DAMJVX010000339 DAMJVX010000000
VERSION     DAMJVX010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.350"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            291..434
                     /locus_tag="PC407_003692"
     CDS             291..434
                     /locus_tag="PC407_003692"
                     /inference="COORDINATES: protein motif:HMM:NF014609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF2499074.1"
                     /translation="MNKKFRLYQVDSFTKERFTGNPAGVITNADGLSESQMQKITREL
                     NNS"
     gene            456..1211
                     /locus_tag="PC407_003693"
     CDS             456..1211
                     /locus_tag="PC407_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HDF2499075.1"
                     /translation="MAKNHQENYDVHIRFFTPTNEVPICGHATIAAHYVRAVENQFNN
                     KTVFQKTGAGILPVDIIKKDNDYEIIMTQGKIEVSPPLQQTYQKRLLNAFGILNEDIR
                     TDCPIAISSTGHSKVMIGIKELSTLNSLSPNLYELSKISQEIKCNGYYIFTLHNKETL
                     QVHGRMFAPAIGISEDPVTGNANGPLGAYLVHYKVIPIPNTEFIFEAVQGEAIKRKGK
                     MLVKVIISDNKPVEVKIIGKAVIAFSTNIEIEL"
BASE COUNT          498 a          163 c          185 g          407 t
ORIGIN      
        1 aacgtatgta agaaccgtag gaactacggg gatagcctgt ggagaattag taagacatat
       61 tttagtatgc aaaattctat gaagcaggaa ccctgtgact ttagtcatgg gaggttcagt
      121 gtagcataga gccttacata atactacttt tttagatgta tatttttaaa ttttaatata
      181 gacatatgtt attttattaa ttttatttaa acagataata ataaataata atttatctca
      241 agttttctat gtagataaac taatctaatg tttagaaagg attatataat atgaataaaa
      301 aatttcgtct ttatcaagtt gactcattta caaaagaaag attcacaggt aatccagctg
      361 gtgtaattac aaatgctgat ggcttatctg aatcacaaat gcaaaaaata acaagagaat
      421 taaataattc ataaactgct tttattttca ataaattggc taaaaatcat caagaaaatt
      481 atgatgtaca tatcagattt tttacaccaa ctaatgaagt tcctatatgt ggtcatgcta
      541 ctattgctgc ccattatgtt agagcagtag aaaaccaatt caataacaaa acagtatttc
      601 aaaaaactgg tgctggaata ttaccagttg atattataaa aaaagataat gattatgaaa
      661 taattatgac acaaggaaaa attgaagtat ctccaccatt acaacaaacc tatcaaaaaa
      721 gattacttaa tgcatttggt attttaaatg aagatattcg tacagattgt cctattgcca
      781 tatcatctac aggacattca aaagttatga ttggaataaa ggaattatca acattaaata
      841 gtttatctcc aaatttatat gaattatcga aaataagtca agaaataaaa tgtaatggat
      901 attatatttt tacattacat aataaagaaa ctctacaagt tcatggaaga atgtttgctc
      961 ctgcaattgg tatttcagaa gaccctgtta caggaaatgc taatggacct ttaggtgcgt
     1021 accttgtaca ttataaagta atacctatac ccaacacaga gtttatattt gaagcagttc
     1081 aaggagaagc aataaaacgc aaaggcaaga tgttagtaaa agtaattatt tcagataata
     1141 agccagttga agtaaaaata attggaaaag ctgtaattgc attctcaaca aacatagaaa
     1201 tcgaattata gattctttct aagtaaattt ttgaactttt taatactaat tta
//
LOCUS       DAMJVX010000340         1196 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.351, whole genome shotgun sequence.
ACCESSION   DAMJVX010000340 DAMJVX010000000
VERSION     DAMJVX010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.351"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            377..889
                     /locus_tag="PC407_003694"
     CDS             377..889
                     /locus_tag="PC407_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499076.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEDYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKAKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
BASE COUNT          537 a           84 c          208 g          367 t
ORIGIN      
        1 ttcagtaaaa taattttgat atactctata tattttgtat gttaaaagta aataaattat
       61 tgttaaagaa tgtaaagact tttaaatttt ttatcacagg attttaagta aataagaagg
      121 attggagtaa ttaaaaattt ttattttgca aaaataagta tttatttata tggataaatt
      181 tgattttaaa tctatcaata tagataaaat cttattattt aacttcaact attccttaaa
      241 aaagcaactt ttaaggaata gttgaaacaa aaagtttggt tacataatag taattttagt
      301 tacaagaatg aaactgtaaa acttaggatt ttctttatat aatttcattg agaacattta
      361 atttaggagg taaggtatga aagtaaaaaa atttaaaagt ggtatagtaa tggcattaac
      421 agtaatggca attggagcat cattgacaaa tgtatcagct atggaattaa acaagagtaa
      481 agagtttgaa actaaataca atgaaatgaa gagggatttt caagaggatt atggaaaaga
      541 agacaaattt aatataacat tggatgatga atttacaaaa gaagaagtaa atggagctaa
      601 agggattata aagacaacta ataaaatgaa taatgaggtt gtagtattaa attattttga
      661 tgaaatgaaa tctgggattg aatttgagtt gaaggcaaaa gcagaaaaaa atgcaatttt
      721 gagagatgaa tttcaacaag gttatggaaa ggaagataaa ttcaaagttg tgttagatga
      781 tgaatttaca aaagaagagg taaatggagc taagggcatt ataaagacta cagataaaga
      841 gacaggtgag gttatcgtat ataattactt tgatgagctt gaaaaataat gcttaaagat
      901 tactttgatt gaatgaaaag gtaattgtgt tgagaatatt aaatgactga ataaaaacag
      961 ttgtatcaaa aacaaacaat aatagaatgt aagaatgatt gtgtcgaaaa tattaaatga
     1021 ctgaataaaa aatagttgcg tcaaaaacaa acaataatag aatgtaagaa tgattgtatc
     1081 aagaatataa aatgattcaa caaaaaacga ttgtgttaaa aacaaacaac aatagaatgt
     1141 aagaatgatt gtatcaagaa tataaaatga ctgaataaaa acgattgcgt caaaaa
//
LOCUS       DAMJVX010000341         1179 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.352, whole genome shotgun sequence.
ACCESSION   DAMJVX010000341 DAMJVX010000000
VERSION     DAMJVX010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.352"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            39..296
                     /locus_tag="PC407_003695"
     CDS             39..296
                     /locus_tag="PC407_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499077.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(406..1086)
                     /locus_tag="PC407_003696"
     CDS             complement(406..1086)
                     /locus_tag="PC407_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2499078.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          481 a          146 c          135 g          417 t
ORIGIN      
        1 tagaaattaa aatgaattaa gtaaatggag gaattgatat gataataatt agaagtcaag
       61 ataaaactaa tttaatgcag ataaaccaaa ttaaaataga tggtagtcaa gtttatgcag
      121 tatttgaaag taaaattgat actgtaaaaa taggagatta tgaaaataat acaagagcta
      181 tacaagtgtt ggacaatata caaaacttca ttgaaaatgg aacaaaatat gattatataa
      241 catcaaataa agtcagatac aatgttaata aaatatttca gatgccagct aaatagtgag
      301 tgtgcatttt agtttaataa ctaaagccca ctttcttttg tagtatgaaa gagggcttta
      361 catatttgta aatatcgaag tacacttgtt ttatcaataa attatttaat aaggtataat
      421 ggaccaatta aacaataaaa attctcctaa aacagcaagt attaaatata taatagcttc
      481 atttttgtaa agagaggtaa tttttttgct tatgtcatca tcaattgtaa tagcaatttc
      541 atcttgaata atatttttat atatagatgg gaaaatatct tttttcttta taagattaaa
      601 taaattattt acacctaccc atataaaata tcctaaaaca gcacctaatg tgttcatcat
      661 taaatcatca atatctgtag ctcttaagtt aaacaattga caaagttcaa ttgctaatga
      721 aaaacctaat ccagttaatg aaacattgat aatatttcta aaatttttcc aaattaaagg
      781 tagtaaaaat cctaaaggca taaacataat tatatttaaa atatatgtta agatgccaac
      841 tgagtcaaaa ggtattagat ttatttcatc aattctaaaa agcccactat accttcctat
      901 atcccatatg ctaccaattc cagctttact aagtgctaga tagatatata ataaaaaaat
      961 atatacccat actaaatgta tcattacatt cttttcaact ttcaaatttt ttcgttttat
     1021 aacgactatt tggtatatta agcatggtaa tataacgcaa ataaatgaat aaaatgtcca
     1081 tatcatttta ttttgtcccc tttcaaattt atgtttttag ttttataagt actatcttta
     1141 tattaaactg taagttttaa aaatctttta ataaaatct
//
LOCUS       DAMJVX010000342         1161 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.353, whole genome shotgun sequence.
ACCESSION   DAMJVX010000342 DAMJVX010000000
VERSION     DAMJVX010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.353"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            29..1069
                     /locus_tag="PC407_003697"
     CDS             29..1069
                     /locus_tag="PC407_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDF2499079.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT          455 a          141 c          192 g          373 t
ORIGIN      
        1 taaatatatt tatttaggag gaaacaatat gaatttaaat ttaactaata ctataaattt
       61 aatgaaaaaa tatttggaca ttccaagccc agctggatat actgaaaatg cagttttaga
      121 aatcaaaaaa gattttgaaa atttaggtct tgacactatt cttacaaaaa aaggtgcact
      181 tatagctact cttcctggtg aagatgattc aaatcaaata acaatatctg cacatatgga
      241 tacacttgga gctatagtaa aagaaatatc atctgatggt actcttaaat accataaagt
      301 tggtggtgga tgttgggcag ctatagaggg agaaaactgc actgttataa ctagaaaagg
      361 taaaaaaatc agaggaagtg ttgtatttaa gtatgcttct actcatatat atggtcaaac
      421 aaaagctgcc actgaaagaa atgaagaaac aatgattatt agacttgatg aaaaagtatt
      481 tacaaagcaa gatgtattag atttaggaat aagtgttggg gattttgtat gcctagacca
      541 tagatttgag gctacagaat ctggttttat aaaatctaga tatatagata ataaatcagc
      601 tgtagcaatg gttcttgaaa tatgtagata ttttaaagaa aacaatttaa cgcctaagca
      661 tactactaac ttttatataa gtaattatga agaaattgga catggtgttt ctaaatgttt
      721 acctgaaaaa acaaaagaat ttgtagcaat agatatagca cctactggac caagtcaaac
      781 atctagtgaa catggtgtaa ctatagttgc aaaagataat ttatcattat atgattttaa
      841 tttaagaaac aagcttgtcg atgttgcaga agacaataat ttagactacg ctgtcgatgt
      901 atttactttc tatggttcag atgcttcaga agctattcgt tggggagaag atgttcaaat
      961 agcatgtgtt ggtcctggaa caaacaatag tcaccattat gaaagaacac atatagaagc
     1021 tattgaaaat acactaaaat tacttataaa ttatatgctt aaagaataat taattatata
     1081 ttttaccaaa gtcaattctt aacaaataat taactttaat ttttattttt atacaaaaat
     1141 tggtttttct atagacttaa t
//
LOCUS       DAMJVX010000343         1157 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.354, whole genome shotgun sequence.
ACCESSION   DAMJVX010000343 DAMJVX010000000
VERSION     DAMJVX010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.354"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     misc_binding    103..366
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            517..1068
                     /locus_tag="PC407_003698"
     CDS             517..1068
                     /locus_tag="PC407_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HDF2499080.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          459 a          123 c          190 g          385 t
ORIGIN      
        1 ttataaaaaa tagattacaa aatatatttt ttataaaaag gtattgacat tttttagaaa
       61 aaagaatatc attattttaa caacaaaatt caatacaata aattaaatcc tatgacaagg
      121 aagagtagtt aaatttaggt tcagtataga gagctgagga tggtggaatc ttagcaagaa
      181 aaaatttgat gaatggccct tagaggaata aattgaaatc atagttttga gagtagactt
      241 tatcgtcagt cgcacgttat agcgaataag gtatgtaagt acctgataat gagatgtatg
      301 aaaattgaat tttatattaa tttttatata atttaggtgg caacgcggat aatctccgtc
      361 ctattagttt aggacggagt tttttatttt actgaaaatt taaaagatag aaggtggtga
      421 gaaagatgtt atgaatttca aaagctaaag atgatgatat agtaggttgg caaaaaaata
      481 aattgaaaat taaaaaaata ggaggaagta caaaaaatga aaacaaatac aaaaaaatta
      541 actttaaatg caatactttt agcgatggga ttattaatac atcaaatcac accagctata
      601 ggattaccaa tgaagccaga tgtaccacta gcaatgttat ttgtaatatt ggttttaaat
      661 agagatgatt ataaaacttg tttgatagcg ggtatagtaa caggaatatt tacagcactt
      721 acatcaagtt ttccgggagg tcaaattcct aatgtaatag ataaaactct tacagcaaat
      781 atagtatttt tattaatgaa tattagttat aatatgcctt ttataaaaaa attagctaag
      841 aaaacacaag atactgtagt agttgcaata ataatgccaa taggaactct tgtaagtgga
      901 actatctttt tattagcagc tcaagctata gttggtcttc caggagcttc atttacagct
      961 ttattcttag cagtagtatt accagctgtt ttaataaatc tagttgcagg aatattttta
     1021 ttcaagattg ttagtctatc aataagaaga gttagttatc aagcttaact acgaaataat
     1081 attttacttt ataaagcatt ataataacta aataacataa gaataaagta gattataact
     1141 attttattct tatgttg
//
LOCUS       DAMJVX010000344         1148 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.355, whole genome shotgun sequence.
ACCESSION   DAMJVX010000344 DAMJVX010000000
VERSION     DAMJVX010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.355"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            10..870
                     /locus_tag="PC407_003699"
     CDS             10..870
                     /locus_tag="PC407_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2499081.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
BASE COUNT          418 a          125 c          163 g          442 t
ORIGIN      
        1 taaggagtga tgttattgaa aaaagataga ataacttttt cagaaatttt aatattaatt
       61 gtaggctgta tattaatggc catttctctt aatttatttt ttaatccaca tgcaatcgct
      121 gctggtggta taacaggact tggagttgtt ttaaattcat tattcggtgt agaactttgg
      181 attgtcaatt tacttttaaa tgttccatta ttcatatttg cttacaaaat actttcaaaa
      241 aaagattgtt ttaaaacggt tttaggaata atttttctta ccatagcact taagcttact
      301 gcaaatatgg ctacattaga tataacaaat gatatgtatc ttgcaattat atctggttct
      361 attttaatgg gagttggtca aggacttatt tttagaataa atggctctac aggtggaact
      421 gatttaatgg cactcctact aaataaatat tttcctacat ttagtatacc tgttttaatg
      481 ggtattgtag actgcgttgt agttgtttta tcaggtatag taaatagaca ggttgaaatt
      541 gcactttatt caactgtggc tttatacatt ttagtaaaag ttagtgactt gcttattgaa
      601 ggatttaatt attccaaatc attcactatt atatctgacc tttcaaaaga tataagtaaa
      661 aaaataatgg aagatttaga tagaggtgca actattctaa aaggagaagg tgcatatact
      721 ggagaaaata aaaatgtact tttagtagta gttgaaaaga aagaagttgt cgaattaaaa
      781 aaattagtaa aaaatgtcga ccctaatgct tttattataa ttacagatat acatgaagct
      841 ttaggtaatg gatttaaaaa aatagaataa ctaacaataa atagcaagta taattaacat
      901 atacaaattc aactttttat atgttatatt atacttgcta tttatatatt atactttcta
      961 tttatatatt ataattgtta tttttaagat aaaaattaaa tgtatcaatt attgttttat
     1021 acaagtattt ccaaatacgt tctcatttag atagtgtgct tagaaatttt agaattattg
     1081 agtctaactt tattacttac tttactttat aacaatcaaa tacaaattta aatattataa
     1141 aactacaa
//
LOCUS       DAMJVX010000345         1134 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.356, whole genome shotgun sequence.
ACCESSION   DAMJVX010000345 DAMJVX010000000
VERSION     DAMJVX010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.356"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(272..763)
                     /locus_tag="PC407_003700"
     CDS             complement(272..763)
                     /locus_tag="PC407_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDF2499082.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDENIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          435 a          167 c          101 g          431 t
ORIGIN      
        1 tccaaaagaa ttactggata taataagata gggtatttag ccttttaaga taaataaaaa
       61 tatctgcaac tatatattgc aaaaatttaa aactaattta tatatgctaa tataaatatt
      121 tataaagaca taagacaaat ttacctacag atttaaattt aacttatata ttttaaaagg
      181 ctaatataat atgtattttc ttctatacaa tcaaaaggct tcatatctaa gatatgttat
      241 gacactaata ttcacaattt tttagtaatt atcacattat aaaaaatatt acagctagtc
      301 catcatcaga tgcttcttgt ataaactttt taagctcaat tagatttgcc catacatatt
      361 caaaaaaatc atcttcactc tcatcattca taacaggata aatattattt tctaacatct
      421 cattaatatt aaatcttttt ctaaatgctt gttcttccaa actgttcatt gcttttgata
      481 gtttttttac atcatttgct gtaatcaaca tgacaggaaa ttctccatca atttccataa
      541 gtatgtttcc acttagtact aatttgctaa atatattctc atcatctcca ccaaaaggac
      601 aacctgtcaa agtaaagtga atggcatgcc atgctttatc aatattgata gtactatttt
      661 cgttggcatc ttcaaaaaca aaatcttgta aattttcttc tgcttgctgt aatttaaaaa
      721 cattctcttc acttatttta gtataacatc ctatcatccc cataattaca ttcctcctaa
      781 gtagcgtttt tttaattgta acttataatt aaacttagtt acaataaaat atattgagaa
      841 aagtattatt tcatcttaaa tttaaatacc tatattatca actaaaattc tgtgtttata
      901 gcgtaagtat tattattttt tcctaaaaat taaatgaatc agaagttcta taggcaatat
      961 tgactataaa taagaactat tacttttaaa aaaaatttaa gaaattatac tgtagaatca
     1021 tgctctactt attagcttca aataaaaaat aaatcatgtt ctcaaattaa ataataaata
     1081 acaataaatt taaattttaa actataagag tttctgaagt gttaataagc atac
//
LOCUS       DAMJVX010000346         1130 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.357, whole genome shotgun sequence.
ACCESSION   DAMJVX010000346 DAMJVX010000000
VERSION     DAMJVX010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.357"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(2..73)
                     /locus_tag="PC407_003701"
     tRNA            complement(2..73)
                     /locus_tag="PC407_003701"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Asn,seq:gtt)
     gene            complement(80..153)
                     /locus_tag="PC407_003702"
     tRNA            complement(80..153)
                     /locus_tag="PC407_003702"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(119..121),aa:Cys,seq:gca)
     gene            complement(161..236)
                     /locus_tag="PC407_003703"
     tRNA            complement(161..236)
                     /locus_tag="PC407_003703"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(201..203),aa:Lys,seq:ttt)
     gene            complement(245..321)
                     /locus_tag="PC407_003704"
     tRNA            complement(245..321)
                     /locus_tag="PC407_003704"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(285..287),aa:His,seq:gtg)
     gene            complement(329..405)
                     /locus_tag="PC407_003705"
     tRNA            complement(329..405)
                     /locus_tag="PC407_003705"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(369..371),aa:Pro,seq:tgg)
     gene            complement(429..505)
                     /locus_tag="PC407_003706"
     tRNA            complement(429..505)
                     /locus_tag="PC407_003706"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(469..471),aa:Ile,seq:cat)
     gene            complement(520..593)
                     /locus_tag="PC407_003707"
     tRNA            complement(520..593)
                     /locus_tag="PC407_003707"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(557..559),aa:Met,seq:cat)
     gene            complement(600..675)
                     /locus_tag="PC407_003708"
     tRNA            complement(600..675)
                     /locus_tag="PC407_003708"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(640..642),aa:Phe,seq:gaa)
     gene            complement(679..767)
                     /locus_tag="PC407_003709"
     tRNA            complement(679..767)
                     /locus_tag="PC407_003709"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(731..733),aa:Ser,seq:tga)
     gene            complement(856..931)
                     /locus_tag="PC407_003710"
     tRNA            complement(856..931)
                     /locus_tag="PC407_003710"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(896..898),aa:Gln,seq:ttg)
     gene            complement(939..1015)
                     /locus_tag="PC407_003711"
     tRNA            complement(939..1015)
                     /locus_tag="PC407_003711"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(979..981),aa:Arg,seq:tct)
     gene            complement(1045..1128)
                     /locus_tag="PC407_003712"
     tRNA            complement(1045..1128)
                     /locus_tag="PC407_003712"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1092..1094),aa:Leu,seq:tag)
BASE COUNT          301 a          307 c          258 g          264 t
ORIGIN      
        1 gctccaaggg tggggctcga acccacagcc taccggttaa cagccggttg ctccaccgtt
       61 gagctacctt ggaatatttt ggaggcgaca cccagattcg aactggggat caaggagttg
      121 cagtccactg ccttaccact tggctatgtc gccacctttt tggtgatcta tccgcgactc
      181 gaacgcggga caccctgatt aaaagtcagg tgctctaccg actgagctaa tagaccatat
      241 gaattggggt ggataatgag agtcgaactc acgacctcca gagccacaat ctggcgctct
      301 aaccaactga gctataccca ccttatattg gtcgaggtga agggactcga acccccggcc
      361 ccatggtccc aaaccatgtg cgctaccaaa ctgcgctaca cctcgaattt atattaatta
      421 atttaatgtg gtgggccatc agggactcga accccagacc taccggttat gagccgggcg
      481 ctctaaccaa ctgagctaat ggcccacatt aaattttagt agcggtaata ggagtcgaac
      541 ctatgacctt tcgggtatga accgaacgct ctagccaact aagctatacc gccatatttt
      601 ggtgcccaga ggcggaatcg aaccaccgac acggggattt tcagtcccct gctctaccga
      661 ctgagctatc tgggcatttg gcggagaggg tgggattcga acccacggcc cctttcgaag
      721 tcactggttt tcaagaccag ctccttaaac cactcggaca cctctccaca ttcggatact
      781 taaacttatt ctaaaattga acctaactat atttaaaata aaaatagttc aaatatagct
      841 tagtaataca tcatttggct ggggatgcag gactcgaacc tacgcataca tgagtcaaag
      901 tcatgtgcct taccgacttg gctaatcccc aatatatgtg gtgagcgcac agggattcga
      961 accccggaca cacgccttag aagggcgttg ctctatccag ctgagctatg cacccacaat
     1021 ttatatatgt atatcaaaca aaaatggtgc ggatgaaggg agtcgaaccc ccacgccaat
     1081 ggcgctagat cctaagtcta gtgcgtctgc caattccgcc acatccgcgc
//
LOCUS       DAMJVX010000347         1118 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.358, whole genome shotgun sequence.
ACCESSION   DAMJVX010000347 DAMJVX010000000
VERSION     DAMJVX010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.358"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            205..345
                     /locus_tag="PC407_003713"
     CDS             205..345
                     /locus_tag="PC407_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499083.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(342..713)
                     /locus_tag="PC407_003714"
     CDS             complement(342..713)
                     /locus_tag="PC407_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2499084.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
BASE COUNT          427 a          114 c          138 g          439 t
ORIGIN      
        1 gatttttttg ttattattac gagtttttta ttgcaactgt agaataaaag tattattata
       61 taattaagat aattatctag ggaaattttg gataatcaaa atatatttta taaaatcaaa
      121 atacaattac ataaacttta ttaaaaattt gttgggaatt tctgactagc attaagaacg
      181 taagtttgtt gggggatggt atatgtggaa aagtcaaaca ctactgttac aaaaaaagaa
      241 aaaattgaag atataggaat gaaattagaa catttattga gtgaagatat tactttttat
      301 gaattattac ttgagatttt gtataaagaa aaagaagaga attaattctc ttctttaagg
      361 aatcttttta aataggcatt atcaatcatt ttttctatag cttctctatc ttctttttca
      421 agagaatata ttttttctaa gagctttttt gttttttcac ttactgataa gttctctatg
      481 attaatttat cttctttgct tatttttgta ttatcattta cgcctaataa ataatctgtt
      541 gatgtttcta atgcttttgc taatctaact attatttcac ttttaggttc tcttaaatta
      601 ttttcataac gtgacaagct agcctcagtt ataccgactt tagtagcaag ttctttttga
      661 tttatatcta attcttttcg tagatttgct attctatctc ctaaactttc catatgtaca
      721 tcactcctta ccgaaattat aagttatttt gataaaaagt aaaataaaat taccaaaaat
      781 ataaaaaaaa agggttaaaa taataaaata tattgaaaat taccatttgt atatgtataa
      841 tatgaattat acaaatggta atttgatgag aggtggtatg ttgaaataag aaactgaatt
      901 aattaataaa tatatagaaa gttttgaaaa cccttaattg agatataaac aatatgtaca
      961 aaattttttg caagtaaaat taccaaatgt ataaaataaa tcagtagcat agataatttt
     1021 atgctttaat acattagatt atttaattta gttataatgg tcaaaaatgc aatctacaaa
     1081 taaaacaaag tattatcaaa aaagtattct ttaagaga
//
LOCUS       DAMJVX010000348         1087 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.359, whole genome shotgun sequence.
ACCESSION   DAMJVX010000348 DAMJVX010000000
VERSION     DAMJVX010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.359"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(207..1076)
                     /locus_tag="PC407_003715"
                     /pseudo
     CDS             complement(207..1076)
                     /locus_tag="PC407_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
BASE COUNT          406 a          171 c          105 g          405 t
ORIGIN      
        1 aatacatcaa aaaataaact aaaaaatatc gaaaaataat ttaaaagaca tcaaaactaa
       61 atcttctaat caacaattaa aagtctattt tcttagttta aaaaataaag atagattttt
      121 tcaaaacact ggataaatag gtaaaaaaat aaacagttag taaaataaat ttttactaac
      181 tgtttatttt tattgtttaa tatgtcctat aaatcctcta agtaatattt tccttgcaaa
      241 aactctgatt ctaaacctag atttttaaaa taatcaacca tgcccataca cgcaaatttt
      301 tctgtagaat aatgagtagc acctataaca ttaattaaat tatcttttgc tatttgatgg
      361 aactctaata ctggttcaaa atgttttaaa ggcttagtaa acccagtaat atatagattt
      421 ataccaagtt cagcaacttc tcttgctaca aatggataac ttcctccccc tgctgcaata
      481 gcaactaaac catcttttaa tacagaatct ccataatcat acaatttcac atcatgacca
      541 acagattttt taattaattc cttaatctct tctattgatt ttagtgatgt tttacatata
      601 actcctacct caattttatc gtcatattta caaaatggtt tgactatttc taaacccata
      661 gcttttgcaa aactaacaga agttgaatag tcactatagt tgtctagtgg agaatgtaat
      721 acataaaaag aaattctacg atttttcatc tcttccatat aagatagtgg tatgtcgtga
      781 aatggaaaac cttcatctga tgctatgtat cccattgcgt gatgtgaaaa aattaaaata
      841 tctttttcat ctttatctat aacttgctta attatttctt tatcaggaaa agtagttgtg
      901 tatactttat taatattttg agcattatct aatactaatc atatgtactt ttttctaaat
      961 tctggagtga taaaatcatt ttcaaaattc ataaaactcc aatcatcatt aatattttta
     1021 atattaaatt ctaaatctaa ttttttataa agttcaattg ccttaataat aaccactcct
     1081 ttataat
//
LOCUS       DAMJVX010000349         1049 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.360, whole genome shotgun sequence.
ACCESSION   DAMJVX010000349 DAMJVX010000000
VERSION     DAMJVX010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.360"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            115..867
                     /gene="cwlD"
                     /locus_tag="PC407_003716"
     CDS             115..867
                     /gene="cwlD"
                     /locus_tag="PC407_003716"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HDF2499085.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          467 a          108 c          161 g          313 t
ORIGIN      
        1 aaacatagtg aattaaatag gggcagaaat ataatatcat tacataataa tatattaata
       61 ccaataaata aagatatatc aatattaaaa aaatttaaat gtttgtacta atatatggaa
      121 tattatagtg tttttataag tatacatata cagggtgatg ttgtgagaaa gtacataaaa
      181 catataattt ttagttttgc aatgatatgt ctagtagtag tatccatatt tgaaattaaa
      241 aatatttctg aagatgttat caagtatatg ccagtaacaa acaaaactat aattttagat
      301 gctggtcatg gaggcattga tccaggtgca ttaaataagg ataagagtac atctgaaaag
      361 gatattaacc tagcaataac acttaagctt agagagctta tagaatcaag tggtggcctt
      421 gtaatattaa ctcgagaaga tgacagtagc ctttataaag aagaaaataa taaaactaca
      481 agacaaaaat ataatgagaa tttgaaaaat agaaaagaaa taatatctaa ttctaatgcc
      541 aatatgtttg tctctataca tcttaatgca tttgaacaat caaaatacta tggagctcaa
      601 actttttatc ctaaagacaa acaagacagt aaagaattat cgaagtgtat tcaagaagaa
      661 cttaaaagag tagtagataa gacaaacaac agagaagtca aaccaaggga tgatatatat
      721 cttttgaaag aaaataatat tccatcagta ctgatagaat gtggtttttt atcaaatgaa
      781 aaagagtgta aactcttaac tgatgaaaca tatcaagaaa aaatagcatg ggcaatctac
      841 ataggaatac aaaaatattt aagttgaaat aagtataaaa ataggtctaa atatgtaaat
      901 agcttgtatt aaagcagtta aaatgcatta atataggcta tttttatttt gacaaaaaaa
      961 tatttaaaat aaaagttaaa aagttgttga cttagaataa tatagatgat attatatatg
     1021 agtgcccaaa aggagcacca aaataagac
//
LOCUS       DAMJVX010000350          972 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.361, whole genome shotgun sequence.
ACCESSION   DAMJVX010000350 DAMJVX010000000
VERSION     DAMJVX010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.361"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(244..711)
                     /locus_tag="PC407_003717"
     CDS             complement(244..711)
                     /locus_tag="PC407_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2499086.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDILGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKGIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEIGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          373 a          164 c           63 g          372 t
ORIGIN      
        1 tataattatc ttttataagc taatacttat atttttctaa atttaaatca tccaataaat
       61 attaaattct attataatat gtaaggaaaa tcatgcactt tttaaaaatc caaataaaat
      121 tataacaaat attatattct gtatcttaca aaaacaaaaa ctgtaagtaa atttgtttcc
      181 atacaagttt acttacagcc cttacttcta tacaaaatat agttttactt taatataatc
      241 catctattct aaaactgatt caatatattc gacagttaca tccattcttt tagctacctc
      301 atctattgaa agaccactat ctaaaaaacg tctacacaca gatgacatag tttctccaat
      361 ctcaataata tgcccatcta aatcataaaa tctaattgct ctttgacccc acctatgctc
      421 tatagctaga tgaacataat ctataccttt cattgtactc aatctttcaa caaaagtatc
      481 aaaattatct tcctcaaaat ataactcagc atcatttcca ttgaacttaa cttcactgtc
      541 actcttatta ataaattcta accaaagctt tttttcttgt aatgatatat taccttctaa
      601 tacaacattt tctccaaaat cacagattac ctttaacccc aaaatatcat aataaaactt
      661 ttttgatttc tcaatatctt caactactat taaaacattt aaaaacttca tattacctcc
      721 ttaatataca catactttat ttataatata taattagaat taaactataa aggtttcaat
      781 aaggtctagt ataaagaaaa aaattataag tcttattgaa atagttaagt taattttatc
      841 atataatata aattttaatc ttgtaaaaaa cagacatgtt catactttta ggtttaaatc
      901 tatatgtatt cttattaatt ttgctaaaac tgttattttt gtagactatt ttagattact
      961 gaaatatcta tg
//
LOCUS       DAMJVX010000351          949 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.362, whole genome shotgun sequence.
ACCESSION   DAMJVX010000351 DAMJVX010000000
VERSION     DAMJVX010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.362"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            146..703
                     /locus_tag="PC407_003718"
     CDS             146..703
                     /locus_tag="PC407_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2499087.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
BASE COUNT          391 a           92 c          146 g          320 t
ORIGIN      
        1 ttcaattagt attatattaa attataatta aactgtcaag attttataag agtataaaga
       61 attttataaa ttacaataat tgacgaaact aattagatta gctaaactaa taaagtaatt
      121 aattttaaat gaaagaggga aaatcatgcg tgttagtaga gaaatagtta tacaagctac
      181 atcagatata gttaataaag atgggcttaa caaagtttct ttaaaagtta ttgcagaaaa
      241 gctaggaata cgtacaccat ctttgtacaa tcatatagaa agtctagatg atttattact
      301 tgaagtagct cataaaggaa tgagagagat gaatagtcaa atgataaagg cgactattgg
      361 aaattttgga gatacagcta taaagtgtat aagtatagca tactttaatt ttataatttc
      421 taatcctggt gtttatgaaa caattcagtg ggctacttgg catggaaata atgaaacatt
      481 agaaatcttt gatgaatata aatccttatt agaaaaactt attctttctt gtaatctaaa
      541 tacaaagaat acaggtgaag tattaaactt aataatgagt gtacttcatg gttattcgac
      601 tttgcaactt gggaaatcac ttttgagtaa agaagaagct ataaaaggtt tagccgattc
      661 tattgatact gtgttattag gtattcataa aaaatatgat taaggaggta aataaattat
      721 tttaacttat ttattaaaca cttcattacg gagcatgtaa attaaaattt tatcatttgt
      781 accaaaggaa aatcaacagg agaacagtat attttaagaa tatttatttt caaatgccca
      841 atattgttta caaagaagag atataaagag aaacaacata gtttatgttt aataattgaa
      901 ttaatttata tgttaatcaa aaaggatatc aaaaaataaa ttatagttc
//
LOCUS       DAMJVX010000352          937 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.363, whole genome shotgun sequence.
ACCESSION   DAMJVX010000352 DAMJVX010000000
VERSION     DAMJVX010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.363"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
BASE COUNT          214 a          246 c          258 g          219 t
ORIGIN      
        1 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
       61 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtaaaa ggatctaggt
      121 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
      181 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
      241 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
      301 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
      361 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
      421 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
      481 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
      541 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
      601 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
      661 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
      721 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
      781 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
      841 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
      901 ccgtattacc gcctttgagt gagctgatac cgctcgc
//
LOCUS       DAMJVX010000353          890 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.364, whole genome shotgun sequence.
ACCESSION   DAMJVX010000353 DAMJVX010000000
VERSION     DAMJVX010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.364"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..758
                     /locus_tag="PC407_003719"
     CDS             <1..758
                     /locus_tag="PC407_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase C80"
                     /protein_id="HDF2499088.1"
                     /translation="TGLRTIDGKKYYFNTNTAVAVTGWQTINGKKYYFNTNTYIASTG
                     YTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPANTDANNIEGQAIRYQNRFLYLHDN
                     IYYFGNDSKAATGWATIDGNRYYFEPNTAMGANGYKTIDNKNFYFRNGLPQIGVFKGP
                     NGFEYFAPANTDANNIDGQAIRYQNRFLHLLGKIYYFGNNSKAVTGWQTINSKVYYFM
                     PDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
BASE COUNT          305 a          115 c          155 g          315 t
ORIGIN      
        1 ttaccggatt gcgaactatt gatggtaaaa aatattactt taatactaac actgctgttg
       61 cagttactgg atggcaaact attaatggta aaaaatacta ctttaatact aacacttata
      121 tagcttcaac tggttataca attattagtg gtaaacattt ttattttaat actgatggta
      181 ttatgcagat aggagtgttt aaaggacctg atggatttga atactttgca cctgctaata
      241 cggatgctaa caacatagaa ggtcaagcta tacgttatca aaatagattc ctatatttac
      301 atgacaatat atattacttt ggcaatgatt caaaagcggc tactggttgg gcaactattg
      361 atggtaatag atattacttc gagcctaata cagctatggg tgcgaatggt tataaaacta
      421 ttgataataa aaatttttac tttagaaatg gtttacctca gataggagtg tttaaaggac
      481 ctaatggatt tgaatacttt gcacctgcta atacggatgc taacaatata gatggtcaag
      541 ctatacgtta tcaaaataga ttcctacatt tacttggaaa aatatattac tttggtaata
      601 actcaaaagc agttactgga tggcaaacta ttaatagtaa agtatattac tttatgcctg
      661 atactgctat ggctgcagct ggtggacttt tcgagattga tggtgttata tatttctttg
      721 gtgttgatgg agtaaaagcc cctgggatat atggctaaaa tatatgtttg acaaaaaatt
      781 attcctgtga tactcagaaa ttatttttat ataataaata ttgagattta attaaaagtc
      841 atgtgttatt gtaatacatg acttttagtt aaaatttttc tatcatttaa
//
LOCUS       DAMJVX010000354          874 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.365, whole genome shotgun sequence.
ACCESSION   DAMJVX010000354 DAMJVX010000000
VERSION     DAMJVX010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.365"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            135..677
                     /locus_tag="PC407_003720"
     CDS             135..677
                     /locus_tag="PC407_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2499089.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT          368 a           74 c          146 g          286 t
ORIGIN      
        1 agacaaatat gttaaaaaaa tgataatcac ggagaacttt taccttcttc aattaatata
       61 ttattattag taataacttt aaaattatat tactattata taaaaattgg cttaaaagaa
      121 ggtgaaaaaa gtaaatgaaa atatgcataa cagtaggtca tagtatacta aaaggtggta
      181 aaagtacagg tgtaaatggt atagttgatg aatacaatta taataaaaaa ttagctccta
      241 tgttagctga gatgcttata agtcaaggta atacagtaga tgtaataatt tgtccagaaa
      301 ggcactttat gtcagaaaga gaagaatttt tctatagagt tccaaaagta aacagtggta
      361 aatacgattt attagtagag ctacatttaa ataaagctga tggaacacaa tgtggtacag
      421 aagtgcttta ctatggcact gaaggattag aatatgctaa aagagtatct aatagacttg
      481 gagaattatt tgaaaataga ggagctaaaa aaagagaaaa tttatatata ttgagaaata
      541 caaatccagt tgcaatacag attgaaagtt ttttctgtga taatgtgaat gattgtaaca
      601 aagcaaatga atcaggttat gattatatag cacgattgat tacagaaggt atattgaata
      661 aagatataaa tatgtgagat tatggtgatt taggtattga ttaatacctt ttaatattag
      721 atataaataa aaatacaatg tattagtata atttaaactt tcaaaatata gattagtttg
      781 attattgaca agagatacct cctaaatatt gatttatcag tatatttaag gaggtattta
      841 aatataaaaa tttaattgat ttgctactaa acta
//
LOCUS       DAMJVX010000355          860 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.366, whole genome shotgun sequence.
ACCESSION   DAMJVX010000355 DAMJVX010000000
VERSION     DAMJVX010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.366"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..417
                     /locus_tag="PC407_003721"
     CDS             <1..417
                     /locus_tag="PC407_003721"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2499090.1"
                     /translation="LDLGDRSIVSYVIGKSNNNNLVFETFNKAIEVYPNAKPIFHSDR
                     GYQYTSRVFKSKLLTQGMIQSMSRVGHCIDNAPMEGFWGILKVEMYYLRKFDTYEQLV
                     KAIDDYIYYYNNFRYQKRLNSMSPLEFRKYLSTEIA"
BASE COUNT          320 a          106 c          119 g          315 t
ORIGIN      
        1 ctggatttag gtgatagaag catagtttca tatgttattg gaaaatcaaa taacaataac
       61 ttagtattcg aaacttttaa taaagcaata gaagtatatc caaatgcaaa acctatcttt
      121 catagtgata gaggctatca gtatactagc agggtgttta aatcaaaact tttaacacaa
      181 ggcatgatac aaagtatgtc gagagttggt cattgtattg acaatgctcc tatggaagga
      241 ttttggggaa ttttaaaagt ggaaatgtac tatttaagga aatttgatac atacgaacaa
      301 ttagtcaaag caatagatga ttatatatat tactataata attttagata tcaaaagaga
      361 ttaaattcta tgtctccact tgaatttagg aaatatttaa gtacagagat agcataattt
      421 attttaagta gctctaaaaa aatttaaata tctagggttt tatttgacgt gctatttttt
      481 agatagtaaa ctttaaaata tagctattaa ttatatgaat ataataaaaa aagtactaat
      541 gattgacact agtactttat aaccactttt acatgtttta actgtataaa acagtgggta
      601 tagttcattg gtcagagtgg ctctttaatt tatcgtaaat gtaactggct ataacaccag
      661 ttataatact catcattaga ttttccatag ttttcacctc ctctcataag aaagtgaaga
      721 aacttccatg gaaacaccac tcatagcttt ttcaaatatt tattccttgc catattaatt
      781 atatcataaa tattggaaac ataaaatttt aatacttatc gtctttatta ttatttttta
      841 taattaataa ttttcttttt
//
LOCUS       DAMJVX010000356          814 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.367, whole genome shotgun sequence.
ACCESSION   DAMJVX010000356 DAMJVX010000000
VERSION     DAMJVX010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.367"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            186..692
                     /locus_tag="PC407_003722"
     CDS             186..692
                     /locus_tag="PC407_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HDF2499091.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          330 a           80 c          134 g          270 t
ORIGIN      
        1 atattttatc tatattttac acttataaaa tatgaaaact acttattgcg aaagtaattg
       61 atttgttgta ttataattta catgaattat gtaggacaat caaaagtcag tattataaga
      121 gtacatatat tcaaattttt aacaattaat ttatataatt aaaaaatgaa gggtaggatg
      181 aagtcatggg ttttagagaa gtaaaaattg aggaactaca gtttaatcca tttactaaga
      241 ttggcaaaga atggttactt ataacagctg gtgattcaga aaagtttaac acaatgactg
      301 ctagctgggg tggtgttgga gtctattggg gtaaaaatgt tgttacaact tacataagac
      361 cacagagata tacaaaagaa tttgtagata gtaatgatac atttacagtt gccttctttg
      421 atgaaacgta tagagaggct ttaaatattt gtggtactat ttctggtaga gatataaata
      481 aaatagaaaa agcaggactt acaccatatt ttgtggatga tacggtagct tttgaagaag
      541 ctaatatgat tatagtatgt aaaaagctat atcatggtaa tatgccacca gaaaactttg
      601 atgctaaaga aaatgataaa aaatggtatc cagagaaaga ctatcatact atgtacattt
      661 cagaaattat aaaagttctt gtaaaagaat aattataata aaaaatatat gaaaagaact
      721 tattataagt aaatgactgt gctttcttat ctgatatcta agtagatgct actaactaaa
      781 taaatattaa ttttaaataa atactattat aaaa
//
LOCUS       DAMJVX010000357          810 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.368, whole genome shotgun sequence.
ACCESSION   DAMJVX010000357 DAMJVX010000000
VERSION     DAMJVX010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.368"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     regulatory      7..100
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            183..668
                     /locus_tag="PC407_003723"
     CDS             183..668
                     /locus_tag="PC407_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2499092.1"
                     /translation="MISELMLKNFGKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
BASE COUNT          359 a           67 c          127 g          257 t
ORIGIN      
        1 ttattattca gatataggca atattagata aatctaatga cgcaaaacta tagggactaa
       61 aatctttatg taataaaggt catgtcagcc agttgccaaa aagataataa ttctttttgt
      121 taaaagtatt tttagagtta tatattttaa taacttaata tttggcaaga ggagggctta
      181 agatgatatc tgaattaatg ttaaaaaatt ttggcaaatc tagcatcaat tttaaaacta
      241 aaattaaatt gaatttaaga gaaaaaccag acataaatag tttgaaatta aaaagtatac
      301 cagaaggtaa aattgttaag ttaaaatgtg tagatggaat atgggcagaa gttgagagta
      361 attatgacaa aggctggtta ctttataaat atttagaaag attaagtaat gctaaaaaca
      421 atagtaatga tacaataaaa aatagaaaaa agaattacat aggaaatata agaacaaatg
      481 gattaagttt agaacttaga aatgatagaa cattagaatc taaagttata actacaatcc
      541 ctgatggttt taaagttgaa gtatgttata gcgttggaaa atgggcaaga gttaatatca
      601 ataaaaatga aaaacgatat tctggatatg tatataatca atacatagaa gagcatttaa
      661 gtttataata cgatactagt gagctttaaa tatgttaata aatcacaaaa aagaatccca
      721 ttaatgggat tcttttttgt agaaacatta taacagaact aaagagaata aattaactaa
      781 tttattttta ttataaagta attataaatg
//
LOCUS       DAMJVX010000358          798 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.369, whole genome shotgun sequence.
ACCESSION   DAMJVX010000358 DAMJVX010000000
VERSION     DAMJVX010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.369"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            34..798
                     /locus_tag="PC407_003724"
     CDS             34..798
                     /locus_tag="PC407_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2499093.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKAPTPDIVDILSEFNVNVL"
BASE COUNT          332 a           84 c          131 g          251 t
ORIGIN      
        1 actataatac tatattgcga aaagaggtag aaaatgtttg cagaagaaag aatccaagct
       61 atattaaata tcctaaaaaa agaaggtaga gttactgtca aagagctcag ttctaaattt
      121 aatgttacag aagattgtat aagaaaagat ttaaaaaata tagaaaagat ttcttcaata
      181 agaagaattt atgggggagc tgtactagct agagaaacac tagagaatca ggatacaaaa
      241 gacagaaaag aaattaatat tccaacaaag aaaataatag cagaaaaagc atttaattta
      301 attgaagata gagaaactat atttttagat atctctacaa ttaacatctt gcttgcaaag
      361 ttgttagctg aaagtaataa aaaagtaaca cttgttacaa atatgttgga tatactaaat
      421 gtagttactt caaaaccaaa taatttaaat ataatttcca ctggaggttt attaaatctc
      481 agtttagacg gttttgtcgg tactccaact atagagttta tatcgaaata taagtttgat
      541 aagactttta tgggaagttg tggagtagat gtattcaact cttcacttac aacttttgaa
      601 attgaagatg gtctcacgaa gaaggctata ataaattcaa gtaaaaaagt atttatagtt
      661 atggaagata aaaagtttaa atttgatgga aatttcaagt ttgctcattt agaagatatt
      721 agttctataa ttacagaaaa agcgcctact cctgatatag tagatatttt atcagaattt
      781 aatgtaaatg ttttataa
//
LOCUS       DAMJVX010000359          789 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.370, whole genome shotgun sequence.
ACCESSION   DAMJVX010000359 DAMJVX010000000
VERSION     DAMJVX010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.370"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            73..642
                     /locus_tag="PC407_003725"
     CDS             73..642
                     /locus_tag="PC407_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2499094.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT          307 a           79 c          157 g          246 t
ORIGIN      
        1 atttgatttt gtaggtaata ttatgtataa ttgtattgca ataatttaaa taattaaaag
       61 gagggaattt caatgaaatt accaatagca ttatctaata aacatgttca tttgagtcaa
      121 gctgatatag acgtattatt tggggcgggt catgagttaa ctaaatttaa accattatct
      181 caaccagggc aatatgcctg tgaagaaaaa gtggatttag taggaccaaa aggaacttta
      241 aagggagtta gagtactagg accagctaga gctcatactc aagttgaaat atctttagca
      301 gatggtttcc cattaggagt aaaagcacca ataaaagaat ctggtgatat agctggaact
      361 ccaggtgtta aattagtagg acctgctgga gaagttgaaa tgaaagaagg agttatagtt
      421 gcttctagac atatacatat gagcaatgaa gatgctgcta aatttggtgt aactgataag
      481 caaatagtaa aagttaaaac atctggacca agagcattag tatttgaaaa tgtattagta
      541 agagctagtg atagctttaa tctagaaatg catgttgata tggaagaggg aaatgcagca
      601 ggcgttaaaa atggagattt agtagaatta atagttgatt aatattataa atagacttta
      661 tgctagtaga tattttattt gctagcatat ttttaaatgt aataaatttc tataggataa
      721 atttaatcga ttagattaat ttataaaata aaggggtatt ataaaaaatg taataaaaac
      781 ttacaaaaa
//
LOCUS       DAMJVX010000360          779 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.371, whole genome shotgun sequence.
ACCESSION   DAMJVX010000360 DAMJVX010000000
VERSION     DAMJVX010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.371"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            93..476
                     /locus_tag="PC407_003726"
     CDS             93..476
                     /locus_tag="PC407_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499095.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKVVLKSPEYIGSHPKKPGKSVEFIKKIDNYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNNGRVKQYDG"
BASE COUNT          328 a           75 c          119 g          257 t
ORIGIN      
        1 ttggaataaa ctatacttta aatatagata tttaatttaa ataaatttta cagtattaat
       61 agtaaattat cttagaaagg aatgtgcttt atatgtctac agacaaagtt gttggaatca
      121 tagatgaaga aacggcagaa cttgcaggaa ttgaatatac tggtaaaatt tatgcttctt
      181 ctggtgttat aaaacatata aagaaaaaac atagatgtca actgagtaag gatattttta
      241 atgatataat agacactata aaagtggtat taaagtcacc tgaatatata ggaagtcatc
      301 ctaaaaaacc tggaaaaagt gtggagttta taaaaaaaat tgataattat attttagtag
      361 ctacagaatt agatacaaaa aaaggatatt tatatgtatc ttcaatgtat gctataaaag
      421 aagcaaaatt agaaagtaga gtaaacaatg gaagagtaaa acaatatgat ggttaacata
      481 atattattaa ttttccccaa aatattgaaa aatattatta tataagttat aatattaata
      541 tacacgtaga tatttattag ttagatttga ggttggaaaa ggcacccaat acgctactat
      601 agagtagtta atagagatgc aggatacgcc gccctgccaa atctaattaa attaaaaaga
      661 gaggattaat tcctctcttt ttgttttttt gagatactaa tttaatctat atagaaagat
      721 tgaaggtcat ttatactaaa agttttcagt tatatacatt aaactaatta aataagata
//
LOCUS       DAMJVX010000361          768 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.372, whole genome shotgun sequence.
ACCESSION   DAMJVX010000361 DAMJVX010000000
VERSION     DAMJVX010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.372"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..625
                     /locus_tag="PC407_003727"
     CDS             <1..625
                     /locus_tag="PC407_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HDF2499096.1"
                     /translation="AGEIRNIIIRGIVSQTAIGTIINTATVSSTTPDPNLNNNTSTSE
                     IDISSSADISVIKLANKTEACVEEQIDFVIVVSNAGPENAQNVTLIDNVSDKLKKAVF
                     SLDRGVSFQPWTGSLNIGTLPAGTLRVILLRGIIKSTCLDRLTNIAEVTSTTPDSNLN
                     NNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDFWC"
BASE COUNT          311 a           93 c          124 g          240 t
ORIGIN      
        1 tgctggagag ataagaaata tcataataag gggaatagta agtcaaacag caataggtac
       61 aattataaat acagcaacag taagttcaac tacacctgac ccaaatctaa ataacaatac
      121 ttcaacatca gaaatagata taagttcatc agcagatatt tctgtaataa aattagcaaa
      181 taaaacagaa gcatgtgtag aagagcaaat agattttgta atagtagtat caaatgcagg
      241 accagaaaat gctcaaaatg ttactttaat agacaatgtt tcagataagc ttaagaaagc
      301 agtattttca cttgatagag gtgtaagttt ccaaccatgg actggaagtc ttaatatagg
      361 tacactacca gctggaactt taagagttat tctattaaga ggaattataa aatcaacatg
      421 tcttgataga ttaacaaata ttgcagaagt tacttcaaca acaccagatt caaaccttaa
      481 caataatata tcaagagtgc aagtagaaat aaaacaatgt tgttgtaatg attgctgttg
      541 taacaactgt agtaattgtt gttgcaatga ttgtggttgt aaagattgtt gctgtaattg
      601 ttgtaaaaat gacttttggt gttgatttta taaaataatt ttgtatacct taaattaaac
      661 tgaaataaaa aataaattca catatgagta aaatatttta taaatattaa taaattttct
      721 atctttgata tggaccacat tcgtaatact agttattaaa tgaagtgg
//
LOCUS       DAMJVX010000362          765 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.373, whole genome shotgun sequence.
ACCESSION   DAMJVX010000362 DAMJVX010000000
VERSION     DAMJVX010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.373"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            62..460
                     /locus_tag="PC407_003728"
     CDS             62..460
                     /locus_tag="PC407_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499097.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRRNARRHNIL
                     TKLVLSIIVIFLVIVLVVLIFIKRKKKKFY"
BASE COUNT          349 a           61 c          101 g          254 t
ORIGIN      
        1 taatttttag ttatatatat attataatga actagttaaa aatttttatg agggggaaaa
       61 tatgaataag aagaaaaaat tgattgggat agctatatct tttctactta taatcagtat
      121 tataaatcca ttacaagtag aagcagaagc tattattgag tctagtgtat acatttcttc
      181 aataaaagag aattatacat atgataatac taaatttaaa actattaaat ctaaaagtag
      241 acataaaagt aggaaatata ataaaagtaa agtaaagttg aaaaaacgaa gtataagaac
      301 taaaaagtat ggatatcaaa ctataaataa atataaaaga cgaaatgcaa gaagacataa
      361 tatcctaaca aaattagtat tgtccataat agtcatattt ttggttatag ttttagtagt
      421 gctaatattt attaaaagaa agaagaaaaa attttactaa aaataattaa aagaatatca
      481 tttttctaat cattggtatc tatttaataa tatgatgtaa ttaaaatgga gtaatattct
      541 gtttcaaagg aggcatataa atacaggaat gagtattaaa tgatggttaa ttaagtaaat
      601 ttattgataa atgtacttaa ttaactttga agaatttaat tcctcatgaa tcaaaatata
      661 aaattacatc aacagttatt cacaaaatta atataagaag aattagagcc aaagagcaga
      721 ctattagacc attaaagtaa ttattcatag aattttgtat atgaa
//
LOCUS       DAMJVX010000363          765 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.374, whole genome shotgun sequence.
ACCESSION   DAMJVX010000363 DAMJVX010000000
VERSION     DAMJVX010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.374"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            68..>765
                     /locus_tag="PC407_003729"
     CDS             68..>765
                     /locus_tag="PC407_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2499098.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHEYQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTILENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          284 a          109 c          127 g          245 t
ORIGIN      
        1 tacaacatta ttttatagtt tgattaattc aacaatcact aatatatcaa actgaaagga
       61 gatttttatg gaaaaaaaat atttcgaaat tcatggagtt ccttcaatcc tttggggaaa
      121 tccttcaagt aaattatata tttacataca tggacaatgc ggcaataaag aggaggctga
      181 agcttttgct aatattgcag ttccacatga gtatcaagtt ttaagtgttg atttgcctga
      241 acatggagaa cgtaaaagag aaaaaaattc atttaaccca tggcatgttg ttccagaatt
      301 agtatctatt atggaatatg caaagtgcca ctggaatcaa atttcgttat atgcaaatag
      361 cattggagca tggtttagta tgttaagctt tgaaaaagag aacttaagag agtgtatttt
      421 tgtatctcca attctagata tgcaacagct catttcaaat atgatgcttt gggcaaatgt
      481 atcagaagaa cagcttaaac atgaactaat tatcccaact tcatttgggc atactctttc
      541 atgggaatat ctacaatatg taaaacaaca tcctatcact caatggaatg taccaacaaa
      601 aatattatat ggtaaacatg atgaacttac tgaaatcggt gttgtagaga aatttgtaga
      661 aaagtttaat tgcaacctta ctattctgga aaatggcaaa cattggttta acacaccaca
      721 agaattaaat tcattatatg aatggttaaa tactttagtt aaata
//
LOCUS       DAMJVX010000364          760 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.375, whole genome shotgun sequence.
ACCESSION   DAMJVX010000364 DAMJVX010000000
VERSION     DAMJVX010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.375"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..>760
                     /locus_tag="PC407_003730"
     rRNA            <1..>760
                     /locus_tag="PC407_003730"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          204 a          168 c          239 g          149 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtactag gtgtcggggg ttaccccctt
      601 cggtgccgca gctaacgcat taagtactcc gcctgggaag tacgctcgca agagtgaaac
      661 tcaaaggaat tgacggggac ccgcacaagt agcggagcat gtggtttaat tcgaagcaac
      721 gcgaagaacc ttacctaagc ttgacatccc aatgacatct
//
LOCUS       DAMJVX010000365          755 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.376, whole genome shotgun sequence.
ACCESSION   DAMJVX010000365 DAMJVX010000000
VERSION     DAMJVX010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.376"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(44..>755)
                     /locus_tag="PC407_003731"
     CDS             complement(44..>755)
                     /locus_tag="PC407_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HDF2499099.1"
                     /translation="INIIICEGKKSERVILNDYINTFFYNYNFEYNIKEFENGGDLLK
                     KYPNNVDIIFMDIDLNDENGFNVSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRYL
                     EKPLKYSLFKEAISSYLKECYKNEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHTE
                     KGIYTFKNSMKEIESNLEEHNFFRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSRY
                     KIKLLREKLNSCLVDVII"
BASE COUNT          266 a          105 c           56 g          328 t
ORIGIN      
        1 agatatattc tattagaata gaatataagg gcaaaaattt acctcaaatt attacatcta
       61 ccaaacaact atttaacttt tctctcaata attttatctt ataacgactt acaattattg
      121 aattttgatt tataaataca atattatttt ctatatctag catatttacc ttcattaaat
      181 ttataaggta gcttttatga catctaaaaa aattatgttc ttctagattt gattctattt
      241 ctttcatact attcttaaaa gtatatattc ctttttcagt atgtatagtt aaatcatttt
      301 ttaaaacttc taaatataaa atatcatcta cctttatgaa atatatcttg catttacatt
      361 ttatagataa aatagcttca tttttgtagc attcttttaa atatgatgat attgcttctt
      421 taaatagaga gtattttaag ggcttctcta aatatctata aactcctatc tcataacctt
      481 tctttatgta tttatttgat gaggatacaa atataattct tacactttca tcaaattttt
      541 tgatttcttt tgaaacatta aatccatttt catcatttaa gtctatatcc ataaatatga
      601 tatcaacatt attaggatac ttttttaata aatcgcctcc attttcaaac tctttgatat
      661 tgtattcaaa attgtaatta taaaaaaagg tatttatata atcatttaga attaccctct
      721 ctgatttttt accttcgcaa ataataatat taatc
//
LOCUS       DAMJVX010000366          752 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.377, whole genome shotgun sequence.
ACCESSION   DAMJVX010000366 DAMJVX010000000
VERSION     DAMJVX010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.377"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..>752
                     /gene="tuf"
                     /locus_tag="PC407_003732"
     CDS             <1..>752
                     /gene="tuf"
                     /locus_tag="PC407_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF2499100.1"
                     /translation="ELLELVEMEVRDLLTEYDFPGDDTPIVRGSALMALEDPKSEWGD
                     KIVELFEQIDEYIPAPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLKVQDEVEL
                     VGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAH
                     TKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTME
                     VDLINSIVVEEGLRFSIREGGRTVASGVVATI"
BASE COUNT          310 a           94 c          187 g          161 t
ORIGIN      
        1 gagttattag agttagtaga gatggaagta agagatttat taacagaata tgatttccca
       61 ggagatgaca ctccaatagt aagaggttca gcattaatgg cattagaaga tccaaagagc
      121 gagtggggag ataagatagt agaattattc gagcaaatag atgagtatat accagctcca
      181 gagagagata cagataagcc attcttaatg ccagtagagg atgtattctc aatcacagga
      241 agaggaacag ttgcaacagg aagagtggaa agaggagtac taaaagtaca agacgaagta
      301 gaattagtag gattaacaga agcaccaaga aaagtagtag taacaggagt agagatgttc
      361 agaaaattat tagaccaagc acaagcaggg gataatatag gagcattatt aagaggagta
      421 caaagaaacg agatagaaag aggacaagta ctagcaaaga ctggatcagt aaaggcacac
      481 acaaagttta cagcagaagt atatgtactt aaaaaagaag agggtggaag acatacacca
      541 ttctttgatg gatatagacc acaattctat ttcagaacaa cagacgtaac aggagcttgt
      601 aagttaccag aaggaataga gatggtaatg cctggagata acgtaacaat ggaagtagac
      661 ttaataaact caatagttgt agaagaggga ttaagattct caataagaga aggtggaaga
      721 acagtagctt caggagttgt tgctacaata at
//
LOCUS       DAMJVX010000367          723 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.378, whole genome shotgun sequence.
ACCESSION   DAMJVX010000367 DAMJVX010000000
VERSION     DAMJVX010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.378"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..>723
                     /locus_tag="PC407_003733"
     CDS             <1..>723
                     /locus_tag="PC407_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDF2499101.1"
                     /translation="KNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGD
                     STTMYPSQNISDEIKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRT
                     VPYISKVSGVPIVDLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEV
                     SLGPEMKSTGEVLGVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAK
                     DMKELGYTFVATEGTAKSLRENG"
BASE COUNT          292 a           75 c          154 g          202 t
ORIGIN      
        1 aaaaatccag tacttataga taaatatcta actggtagag aaatagaagt agatgctata
       61 tgtgataagg aagatatatt gatacctggt ataatggaac atttagaaag agctggagtt
      121 cactctggag atagtacaac aatgtatcca agtcaaaata tatctgatga aataaaagaa
      181 aagatagtag aatacactaa aaaaatagct ttagacctta atgtgcttgg tatggttaat
      241 atacaattta tagaatttca aaatgaatta tatataattg aggttaaccc tagagctagt
      301 agaacagttc catatataag taaagtaagt ggagtaccaa tagttgactt ggctactaag
      361 tgtatgttag gagctaaatt aaaggattta ggatatggta caggagttta caaagagcca
      421 aaattagtat cagtaaaagt accagtattc tcaatgtcta aattatctaa agttgaagta
      481 agtctaggac ctgaaatgaa gtctacagga gaagtcctag gtgtaggaga aaatttagaa
      541 gaagccttat ataaagggtt cttggcagca ggtagacata tgtctgatga aagaggagta
      601 gtacttgcta ctgtaaataa tcatgataaa gatgagttta tagagatagc aaaagatatg
      661 aaagaattag gatatacttt tgttgctaca gaaggaacag cgaagagttt aagagaaaat
      721 gga
//
LOCUS       DAMJVX010000368          700 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.379, whole genome shotgun sequence.
ACCESSION   DAMJVX010000368 DAMJVX010000000
VERSION     DAMJVX010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.379"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..423
                     /locus_tag="PC407_003734"
     CDS             <1..423
                     /locus_tag="PC407_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2499102.1"
                     /translation="TISKKYKMRSVNPLKAWVKEYNLHKEIKETPKGGLFMETRKTTL
                     DERVEIVKDCIEHDFNYNLIAKKYFVSYSQVRRWTLKYKENGIVGLEDRRGKAKKEDE
                     LTQLDEIKLELKIVKAEKHKLQLENAFLKKLKNIERGW"
     gene            417..>700
                     /locus_tag="PC407_003735"
     CDS             417..>700
                     /locus_tag="PC407_003735"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2499103.1"
                     /translation="MVITLSKNENKYKAIQSLFNEKKASLMQLCEIAKVNRSGYYKWL
                     NRDKSNLELENIKLATLITNIYEEKKGVFCSLRMTLQLNREYKLNVNHKRV"
BASE COUNT          307 a           78 c          126 g          189 t
ORIGIN      
        1 actatctcaa aaaaatataa aatgaggtct gttaatccac taaaagcttg ggttaaggag
       61 tataatcttc ataaggagat aaaagaaacc ccgaagggag gactctttat ggagactaga
      121 aaaacaacat tagatgaaag ggttgaaatt gttaaagatt gtattgagca tgattttaat
      181 tacaatttaa tagcaaaaaa atactttgtt tcatacagcc aagttagaag atggactctt
      241 aaatataaag aaaatggtat agttggttta gaagaccgaa gaggtaaagc aaaaaaagaa
      301 gatgagttaa ctcaattaga tgaaataaag ttggaattga aaattgtaaa agcagaaaaa
      361 cacaaattac aactggagaa tgcattctta aaaaagttaa aaaacataga aagagggtgg
      421 taattacact atccaagaat gaaaataaat ataaagctat tcaatcctta ttcaatgaaa
      481 aaaaagcttc gcttatgcaa ttatgtgaaa tcgctaaagt aaatcgttct ggatactata
      541 aatggctcaa tcgagataaa agtaatttag aacttgaaaa cattaaatta gcaactttga
      601 taactaatat atatgaagaa aagaagggag ttttttgctc tttaaggatg accttgcagt
      661 taaacagaga gtataaactt aatgtcaatc acaagagagt
//
LOCUS       DAMJVX010000369          639 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.380, whole genome shotgun sequence.
ACCESSION   DAMJVX010000369 DAMJVX010000000
VERSION     DAMJVX010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.380"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(<1..618)
                     /locus_tag="PC407_003736"
     CDS             complement(<1..618)
                     /locus_tag="PC407_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499104.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWENDEQCKSLDFKDLNNNK"
BASE COUNT          200 a          115 c           35 g          289 t
ORIGIN      
        1 tttattatta tttaaatctt taaaatctaa acttttacat tgttcatcat tttcccatat
       61 tcctgcttgt ttatttccat catttttata taatacaccc ttaccatttt tcttattatc
      121 taaaaaatat cccttataaa tgtcattatt gggatatgtt aattttccca aaccattttt
      181 taatccgtat ttaaattcac ctgtatatat tgaaccatct ctataagtat attttcctat
      241 tccatgcatc ttgtcatctt tccataatcc ttcatacttt tccttacttg aaaaaatata
      301 tattccaaat ccatttttct ttccatcaac aaactcacct ttatataaat caccattatc
      361 gtatttaaat actttttctt cctttaattt cttattgtta ctcttatttt tattattatt
      421 gttatccttt atatgtattt cttctcttct ggcttcaata aaatctctat catacctaaa
      481 tccgtttctt gatttaattt tttgattaat cttcttctta ttaataaata ctctagctag
      541 ttgagtaaat ataaatacag atgataagca tataaataca catagtattg ttgtaaaata
      601 actagttata ttttccataa aaaatcaccc tttaaaaaa
//
LOCUS       DAMJVX010000370          632 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.381, whole genome shotgun sequence.
ACCESSION   DAMJVX010000370 DAMJVX010000000
VERSION     DAMJVX010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.381"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            126..494
                     /locus_tag="PC407_003737"
     CDS             126..494
                     /locus_tag="PC407_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HDF2499105.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNKVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT          264 a           55 c          104 g          209 t
ORIGIN      
        1 aaatttattt atagtgaata taggttggat tttatagata caaaaatagt atatatgata
       61 tgtagaccat tagcatgttt aaagtagcaa aacatataaa ataaaatttg aaaggaagta
      121 taaaaatgac aagaccatca atatatcata gccaaggata cacttgtgca gaggcattaa
      181 taaagtctta taatgaggaa cacaacacag atataccaat ctcaatagga agtggtatgg
      241 gggttggaat gaatgttgga agtgtttgtg gagctgtaaa tgctgctgtt gttataatag
      301 ggtatttaaa tgggcgaaat agtaatttag atgaaaatat agcaaggaag tattcaagag
      361 agctattgaa taaagtaaga aataaataca gctcagaaat atgtgtatct cttaaaaaaa
      421 ataaagtaac ttgtgaagaa ataataaatt tcacatatga tgctctaaat gatatgctaa
      481 aaaatcaaaa ataattctaa gaaacaagta ttttacattc gatagaatac ttgttttttt
      541 atgatatttt taataggtat tagtttatta taatttttaa atcaatttta ttatatttta
      601 attgaaataa tctactttga aaattactaa ct
//
LOCUS       DAMJVX010000371          616 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.382, whole genome shotgun sequence.
ACCESSION   DAMJVX010000371 DAMJVX010000000
VERSION     DAMJVX010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.382"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(181..357)
                     /locus_tag="PC407_003738"
     CDS             complement(181..357)
                     /locus_tag="PC407_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499106.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(411..500)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          238 a           93 c           56 g          229 t
ORIGIN      
        1 ttgtgtatta ccacaaatac tattatatca tagattaaca taaatgtcta tttaattata
       61 atttttagtt aacattttta tttattctta atatgaaaat aaatttaaac attttatatt
      121 accaatatat taatgtaaat gtagataaaa ctaaaaaggg ttttctagaa gaaaatctgt
      181 ctaattatac ttacttacca ataccatcac ttgatgcaag caagattcca caactttttc
      241 tccgtactct aatataaaat catctaactg tgtatctata aaatcacaat aatcatatga
      301 actaccaata taagcagtta gcatatgtgt acatttatcc attttatctc taaacataat
      361 gtatttatcc ccctaaaaaa ctttcatgaa aaacaaaaag aaaatatctt tttggcaact
      421 ggctgacata acttatttta taagcgttag tccctatagc tttgcgtcac taaatttctc
      481 tagttttgcc gatttagttt tattctacag attaataata caatagaatt agctattatt
      541 caacatatct agctgaaaaa ttaataaatt tatatatgaa cgttaaatta tttgaaaaaa
      601 taaaaagccc ctctgt
//
LOCUS       DAMJVX010000372          614 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.383, whole genome shotgun sequence.
ACCESSION   DAMJVX010000372 DAMJVX010000000
VERSION     DAMJVX010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.383"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..>614
                     /locus_tag="PC407_003739"
     CDS             <1..>614
                     /locus_tag="PC407_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499107.1"
                     /translation="TGWQTIDGKKYYFNPNNAIAATHLCTINNDKYYFSYDGILQNGY
                     ITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQNKFLTLNG
                     KKYYFDNDSKAVTGWQTIDSKKYYFNLNTAVAVTGWQTIDGEKYYFNLNTAEAATGWQ
                     TIDGKRYYFNTNTYIASTGYTIINGKHFYFNTDGIMQIGVFKGP"
BASE COUNT          225 a           80 c           99 g          210 t
ORIGIN      
        1 ttactggatg gcaaaccatt gatggtaaaa aatattactt taatcctaat aatgctattg
       61 ctgcgactca tctatgcact ataaataacg acaagtatta ctttagttat gatggaattc
      121 ttcaaaatgg atatattact attgaaagaa ataatttcta ttttgatgct aataatgaat
      181 ctaaaatggt aacaggagta tttaaaggac ctaatggatt tgagtatttt gcacctgcta
      241 atactcataa taataacata gaaggtcagg ctatagttta ccagaataaa ttcttaactt
      301 tgaatggcaa aaaatattat tttgataatg actcaaaagc agttactgga tggcaaacta
      361 ttgatagtaa aaaatattac tttaatctta acactgctgt tgcagttact ggatggcaaa
      421 ctattgatgg tgaaaaatat tactttaatc ttaacactgc tgaagcagct actggatggc
      481 aaactattga tggtaaaaga tactacttta atactaacac ttatatagct tcaactggtt
      541 atacgattat taatggtaaa catttttatt ttaatactga tggtattatg cagataggag
      601 tgtttaaagg acct
//
LOCUS       DAMJVX010000373          610 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.384, whole genome shotgun sequence.
ACCESSION   DAMJVX010000373 DAMJVX010000000
VERSION     DAMJVX010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.384"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            1..>610
                     /locus_tag="PC407_003740"
     CDS             1..>610
                     /locus_tag="PC407_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2499108.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNISI"
BASE COUNT          212 a           55 c           81 g          262 t
ORIGIN      
        1 atgttagatg catttataac aattgtttct tctattatag aaatatggtc atgtaaaaaa
       61 gtttttgatt atactagtca gtcaaagacc gacttaaaaa aactaaatat gtattttctc
      121 ttttctgttt ttcttattat ttcttcttta tatataaata taggagctaa tagtagaatc
      181 ttgatatgta tatttgtaat gttcgttttc tataagtcta actataaggt taatatatat
      241 aaatgtctgg taatctcatt tttttattgg ttaatattaa taggtataaa tggattaagt
      301 atgttattag tagcaacagt aagcaatttg gataatatga atgttttact tagtggcagt
      361 ttatatagat atgaagcaat attcttggga aagataatgt taggtatatt actttatgtt
      421 tataaggctt cttttaaaaa ttttaaaata agtaaaaaag agatatacat atgtattcct
      481 atgatagcaa atttattgtc attttttgta ctttatgatt atattattaa tcttgtaaaa
      541 atctctcctg aaagaagaac tgctttttta gtaatagttt tactattaat attatctaac
      601 atttcaatct
//
LOCUS       DAMJVX010000374          591 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.385, whole genome shotgun sequence.
ACCESSION   DAMJVX010000374 DAMJVX010000000
VERSION     DAMJVX010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.385"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            27..500
                     /locus_tag="PC407_003741"
     CDS             27..500
                     /locus_tag="PC407_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2499109.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFEDIAVEQIVDGIRFTPMKLLI"
BASE COUNT          233 a           57 c           80 g          221 t
ORIGIN      
        1 aaataataat aaaggtgttg tttataatgt tcaaagaata cttaataaga gaattaaaag
       61 aagatgatat taaaaatgca ttagacttaa tctggaatgt atttgaagaa tttgaatctc
      121 cagattattg tgatgaagga attacgacat ttcatcaatt tatagaatat aattctatta
      181 ttgaaaaaat caaagaaaaa acactcttct tttggggttg ttttgactct aaaaaactta
      241 ttggagtctt agctattaga aatttaaatc atatatgtat gttatttgta gataaaaact
      301 atcataaaca tggtgttgca aaaaaattaa tgactacagg catacaatac tgtatttgta
      361 ataaatttaa tttaactgaa ataactgtaa attcatcacc ttatgctgtt gaagtttatc
      421 ataaattagg atttgaagat attgctgttg agcaaattgt tgacggtata cgttttacac
      481 ctatgaaatt gttaatataa taaattaaat aaaaagcaaa gtctcttaaa tattttatag
      541 ttactttgct tttaaaccaa gatttaaaat aatactatct tttttgtttt t
//
LOCUS       DAMJVX010000375          587 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.386, whole genome shotgun sequence.
ACCESSION   DAMJVX010000375 DAMJVX010000000
VERSION     DAMJVX010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.386"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(81..560)
                     /locus_tag="PC407_003742"
     CDS             complement(81..560)
                     /locus_tag="PC407_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2499110.1"
                     /translation="MPNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTISGILMFILGVSYFIIIKSLIEVLSSSEHSLFVKDNVKRFRIIGYLLLLNSIMEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          233 a          102 c           46 g          206 t
ORIGIN      
        1 accctaatat attttatttt aaattactag ttaatcactc ttttctggtt atttatctcc
       61 tatatattaa agtgtcacac ctaaatagtt aaatcattat cctcttttat ctttattgcc
      121 tttacaaatc catctgcaat tacaaaactc ataagagaag ttataaaata tacaaatact
      181 ggaacagtaa aataaaatcc aaatcctaaa tctaggaatc tcattccttt tccagtagta
      241 ccaaaagttg aaataaactc cattatagag tttaaaagca aaagatatcc aataatccta
      301 aatcttttta cattatcttt tacaaataag ctatgttcag aactacttaa tacttctatc
      361 aatgatttta taatgataaa atatgataca cctaaaataa acattaatat tccagaaata
      421 gtgccttgta gagttgcttc tccactaaaa cagaggtaca taaaactttt aaatcctccc
      481 acaacaatta ctaaccctaa aagaaaaaat aaaatttgta atataccact tagtaaattt
      541 tttataagtt tattaggcat cataccaact cctttaaaat attttat
//
LOCUS       DAMJVX010000376          577 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.387, whole genome shotgun sequence.
ACCESSION   DAMJVX010000376 DAMJVX010000000
VERSION     DAMJVX010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.387"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            252..>577
                     /locus_tag="PC407_003743"
     CDS             252..>577
                     /locus_tag="PC407_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2499111.1"
                     /translation="MNLQSKWVNFLKPTYVSDEIKRKKLQVIIFFSITYGLSYSLGLL
                     AICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFYSSVILFFIIYFFIFI
                     IESLKL"
BASE COUNT          235 a           46 c           68 g          228 t
ORIGIN      
        1 aaaaaaataa tagtaaactg attgcataaa aatgattttt atataaattt taaacagaag
       61 aataaatttc taacgaaatt ggatatttct tctgtaaata catattcaaa aaaagaatta
      121 taagagttta gtcagcagtc gtgtaagtaa taaggacttt ataaatatat agaaatataa
      181 tgatatgagt ataagtagaa aaattttatg atttttacaa ttatttttta taaaaaggga
      241 ggttatgttt gttgaattta caatcaaaat gggtaaactt tttaaagcca acatatgtca
      301 gtgatgaaat aaaaagaaaa aaactacaag ttataatatt tttttctata acttatggtt
      361 tatcttattc actaggttta ttagcaatat gttttaatca tttaatagat tcagaaattt
      421 tagctcaatt tatgatgata ttgccattat cttcagtttc tattgcaaaa ttttacacag
      481 aaggaagaaa taatgataaa tataatttct attcatcagt aatattattt tttattattt
      541 acttttttat atttattatt gaatcattaa agttaat
//
LOCUS       DAMJVX010000377          561 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.388, whole genome shotgun sequence.
ACCESSION   DAMJVX010000377 DAMJVX010000000
VERSION     DAMJVX010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.388"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..161
                     /locus_tag="PC407_003744"
     CDS             <1..161
                     /locus_tag="PC407_003744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499112.1"
                     /translation="KTAFNEETRGRFKNISLENDNRKFKSVEEELKYLRMENEFLKKL
                     STLLDKSK"
     gene            188..>561
                     /locus_tag="PC407_003745"
     CDS             188..>561
                     /locus_tag="PC407_003745"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2499113.1"
                     /translation="MSRKYSISSMCKLIGVSRSGYYKWLSYSKKSSDRGIKDRIIKDY
                     IIEIHKKYRGTYGRKRICTYLNKILDSPINHKKVYRLMKELGIKSIIRKKVYRRKFKS
                     YEVYDNILNREFRANQPLEKIC"
BASE COUNT          243 a           46 c           98 g          174 t
ORIGIN      
        1 gtaaaactgc atttaatgag gaaactagag gcagatttaa aaatattagt ctagaaaatg
       61 ataatagaaa atttaaatct gttgaagaag aattgaagta cttacgcatg gagaatgaat
      121 ttttaaaaaa gttaagtacc ctattggaca agtcaaagta agcgataaat ttaaaacaat
      181 taaacttatg tctaggaagt atagtatttc tagtatgtgt aagttgatag gtgtgtcaag
      241 aagcggttac tataaatggc taagttattc taaaaaatct tccgataggg gtatcaaaga
      301 tagaattatt aaggattata taattgaaat acataaaaaa tatagaggaa cttacggtag
      361 aaaaagaatt tgtacttatt taaataaaat acttgatagc cctattaatc ataagaaagt
      421 atataggttg atgaaagaac taggaattaa atctattatt agaaagaaag tatatagacg
      481 taagtttaaa tcatatgagg tatatgataa tatattaaat cgtgaattta gagctaatca
      541 accattagaa aaaatttgta t
//
LOCUS       DAMJVX010000378          550 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.389, whole genome shotgun sequence.
ACCESSION   DAMJVX010000378 DAMJVX010000000
VERSION     DAMJVX010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.389"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
BASE COUNT          217 a           43 c           71 g          219 t
ORIGIN      
        1 aatgtataag gtggtaaatt tatataaaaa agaatatgag ttgtaaaata ttaaataata
       61 ttgtactaat tggaataatt ttaactttat tatagattat tttaatgcca ttagtactga
      121 tggtgtattt taaaattggt ataagcatta caaatacgaa tatgccaact gtaatatgta
      181 atataagtag ctatatacat atgtgctgtc ccatatattg tatcattgtt tatattaaaa
      241 agattatgta aattggtatc aatcaataaa tcattttcag aagaaatacc taaaaactta
      301 aaaaatacct atatgtgtat ttagttagat tggagatttt tacatttaat tttatagtga
      361 caatattata ttattgttat gatgtctatt tatatgcaat tactataata tcagaactta
      421 tagtatcatt tgtttcatta tcaataaggt tttttacttt gttttgtcaa ttttattaaa
      481 aaaagttatt gatataaaag ataagaagga taaaactata tgacagattt caaacataag
      541 agtggatatt
//
LOCUS       DAMJVX010000379          540 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.390, whole genome shotgun sequence.
ACCESSION   DAMJVX010000379 DAMJVX010000000
VERSION     DAMJVX010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.390"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
BASE COUNT          218 a           74 c           57 g          191 t
ORIGIN      
        1 cccagtgcca taatcgataa aattaaactt tgcgtcatta cagatataat acctgacata
       61 aataaaacac ccctttgaaa atattttaaa tggtttttta aatttggttt taaaaaatga
      121 ttttgatata aaaaaaccac acagtatata taactgtatg gcaaaaattc gaataagcca
      181 cacagtataa tccatgtggc tctatattat gatgataaaa taaaaacttt aacaacaatt
      241 actatagcca acatagatac aatccttaaa aatcataatc ctaaggggct tgtatctatg
      301 ttatgacaat ttgtcttaac ttaggtacaa accctaacta aattggctac taaaaaatcg
      361 tacttgtttg tttaataaag tcttcatatt tacctctcct tttaatttaa taattttgaa
      421 aattttgttt tctgttttgt atagtaatta gtttaataca gtaaaaaata aaagtcaact
      481 ataaattttg aaaaattatc aagttatcag aaaattgagt ttaaataaaa aaatttttac
//
LOCUS       DAMJVX010000380          506 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.391, whole genome shotgun sequence.
ACCESSION   DAMJVX010000380 DAMJVX010000000
VERSION     DAMJVX010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.391"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
BASE COUNT          214 a           56 c           41 g          195 t
ORIGIN      
        1 aaatgttaag ataaaatttt tagatttata tcacaattta ggattatttg taaaaaagga
       61 gattatcttt ttattagaca atccccttgt atattttatt aatatttatc tatgattaag
      121 catcaatttt attttatact ttttgatttt aatactatac atctaaatct tatttagggg
      181 gaacaattat ttatatgata taaaaatgct aatttgaatg aacttgtctt ataatcagtt
      241 ttataaaata caccattcta caaagtatga atcaaaaggt gaaaaaatac taaaaataat
      301 aaatcaacat caattttaac caatatatga aaaacttata ccaagtatgc ataaaaacta
      361 atataaatat tattttaaca actaatactt acacctttat atattatatc atatttcaat
      421 aaataaattg cataaaagga aatcttttca tagaattaaa ccatttatta attcattact
      481 ataggttcaa taaactataa gatgtc
//
LOCUS       DAMJVX010000381          500 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.392, whole genome shotgun sequence.
ACCESSION   DAMJVX010000381 DAMJVX010000000
VERSION     DAMJVX010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.392"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..363
                     /locus_tag="PC407_003746"
     CDS             <1..363
                     /locus_tag="PC407_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2499114.1"
                     /translation="LMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGITL
                     FKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLATGNLNISL
                     KKTNAKLVPKINQKKEVL"
     gene            364..>500
                     /locus_tag="PC407_003747"
     CDS             364..>500
                     /locus_tag="PC407_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2499115.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KS"
BASE COUNT          178 a           71 c           77 g          174 t
ORIGIN      
        1 cttatgattt caaacggact tatagcttta tctggtagtc ttatggctca gttcttggga
       61 ttctcagatg taaatatggg tataggaact ttagttcttg gaattgcctc tatcataatt
      121 ggtattacac tatttaagaa atttactttt atcaaagata ctactgcaat aattgttggc
      181 tcttttatat accaattcac tatatacttt gcaatgagtt taggaatgtt atcaactgac
      241 ttaaaactaa taactgccat agttataata gctttcctag ctactggtaa cttaaatatt
      301 tcactaaaaa aaacaaatgc aaagctagta ccaaaaataa atcagaaaaa agaggtgttg
      361 tagatgttac aaattaaaaa tctttcaaag agtttcccta atccatatgg tgaacctaat
      421 actatctttg aaaatttatc aatcgatata gaagatggtg aatttgtaag tatcataggt
      481 agtaatggaa ctggtaaatc
//
LOCUS       DAMJVX010000382          499 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.393, whole genome shotgun sequence.
ACCESSION   DAMJVX010000382 DAMJVX010000000
VERSION     DAMJVX010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.393"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
BASE COUNT          219 a           49 c           56 g          175 t
ORIGIN      
        1 agacagtttt acatatttca actgtctcct aagaggggta tagttcacaa ctaagagtgg
       61 ctgggaactt aatataaagt tccagaaaag taaatcttca aaataattta ttgaaggaaa
      121 catcactcaa agttaagtaa aatatgtttc cttgctttta ttatatcaca aattaaaaat
      181 attaacacaa aattttgtga atatatactt attattgttc taaaaaaata tgtaaataat
      241 tgatttgatt tcatagaatt attgttgtaa tatttagtta aatatacaca atctcaatta
      301 aagaagataa gtattataaa ttcatatcaa aaaaatacat aaacttttaa gttataattt
      361 tataaaaagt cttttagtat cattatttca agaggaatta acatatgaaa aaataacttc
      421 attagaaaag tggataaaaa actggtaaga agatgactaa agattacata tactattact
      481 taaattaaaa atttaatat
//
LOCUS       DAMJVX010000383          484 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.394, whole genome shotgun sequence.
ACCESSION   DAMJVX010000383 DAMJVX010000000
VERSION     DAMJVX010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.394"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..>484
                     /locus_tag="PC407_003748"
     CDS             <1..>484
                     /locus_tag="PC407_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2499116.1"
                     /translation="VFYFLFSQIIFGLIISDNQFDYKNFLNYILLPIISLVYIYPFLC
                     EEYGWRSFLQSILFDRFGKKIGIVMVGTCWSLWHLPLQFTLYSPQTPIIGAIAHLIYG
                     IGLSIFLGYVYMKTKNIWFCSIMHVLINSLGLIFNDSEIIINYHSIIQRLIFILLFYT
                     P"
BASE COUNT          154 a           52 c           64 g          214 t
ORIGIN      
        1 agttttttac tttttatttt ctcaaattat ttttggatta ataataagtg ataatcaatt
       61 tgattataag aatttcttga attatattct tcttcctata atatcattag tatatattta
      121 tcctttttta tgtgaagagt atggttggag atctttttta caaagtattt tatttgatag
      181 atttggcaaa aaaataggta ttgtaatggt aggcacttgt tggagtttat ggcacttacc
      241 tttacaattt acactatata gtcctcaaac tccaataatt ggagctatag cccatttgat
      301 atatggtatt ggactttcta tatttttagg ttatgtttat atgaaaacta agaacatatg
      361 gttttgctct ataatgcatg ttctaattaa tagcttagga ttaattttca atgattctga
      421 aataattatt aattaccata gtattataca aagattgata tttatactat tattttatac
      481 tcct
//
LOCUS       DAMJVX010000384          468 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.395, whole genome shotgun sequence.
ACCESSION   DAMJVX010000384 DAMJVX010000000
VERSION     DAMJVX010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.395"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(148..447)
                     /locus_tag="PC407_003749"
     CDS             complement(148..447)
                     /locus_tag="PC407_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499117.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
BASE COUNT          176 a           64 c           51 g          177 t
ORIGIN      
        1 ttataaacta atatatttta caaattgttg agtaatattt tatgatataa atcagaagaa
       61 gcacttctat aatagaaagt gctcaactat gaataagatt aataatatat ttgccttaga
      121 tatatagact tttataaata ttaaagatta atcaaatctg atgtttgaaa ctttatccca
      181 attaatcttc cttgaatagt cttctggatg tgataatcta actatatgag ttgaattatc
      241 atagctgtaa taaattgtag tagtaccatc aactggattc tttttgtatc cttgatatac
      301 agcactattt ggtactttct tttcaacaaa gctatatgca gatgaagtat caatagtttc
      361 tattttatta ggaaatataa acatataaaa agctactact ataaaaatac atatgcatgt
      421 aatactatac catttaatcc atttcataaa attcctccta ctttacca
//
LOCUS       DAMJVX010000385          451 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.396, whole genome shotgun sequence.
ACCESSION   DAMJVX010000385 DAMJVX010000000
VERSION     DAMJVX010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.396"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(<1..423)
                     /locus_tag="PC407_003750"
     CDS             complement(<1..423)
                     /locus_tag="PC407_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HDF2499118.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKDINDT"
BASE COUNT          149 a           59 c           44 g          199 t
ORIGIN      
        1 tgtatcgttt atatcctttt ctactaatgg tttcatatta ttattataaa tacttatatc
       61 tgcattactt aattccattt ttatgcgatt aataagattt tctttatact ttgtaatatt
      121 aggtatggat ttaataaaca ttcttgatac agaaggtttt ttatctacga tatgacttag
      181 tatactatct atatttattt caattttatt atcaatatcc cactgagcat ttgcagagat
      241 taataacaat ccccttgttc gaatatatga gttttcgtta tcaatcattt ctaggaatga
      301 gtcaaaaaaa caatatgtaa tatttgattt taaactttca ttttctaaga ataaaaaagt
      361 tttatatgct tcattatgat tttttgaata taatttatta ataagtaaat tgagattctt
      421 caatcttctc atctccctca ttacaaatat t
//
LOCUS       DAMJVX010000386          420 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.397, whole genome shotgun sequence.
ACCESSION   DAMJVX010000386 DAMJVX010000000
VERSION     DAMJVX010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.397"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..>420
                     /locus_tag="PC407_003751"
     rRNA            <1..>420
                     /locus_tag="PC407_003751"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          106 a           97 c          130 g           87 t
ORIGIN      
        1 cgcctcccaa aaggtaacgg aggcgctcaa aggttctctc agtacggtcg gaaatcgtac
       61 gtagagtgta aaggcaaaag agagcttgat tgcaagacat acaggtcgag caaggatgaa
      121 aatcggactt agtgatccgg tggttctgcg tggaagggcc atcgctcaac ggataaaagc
      181 taccctgggg ataacaggct tatctccccc aagagtccac atcgacgggg aggtttggca
      241 cctcgatgtc ggctcatcac atcctggggc tgtagtaggt cccaagggtt gggctgttcg
      301 cccattaaag tggtacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatcc
      361 gtcgcaggcg taggaaattt gagaagacct gtccttagta cgagaggacc gggatggacg
//
LOCUS       DAMJVX010000387          416 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.398, whole genome shotgun sequence.
ACCESSION   DAMJVX010000387 DAMJVX010000000
VERSION     DAMJVX010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.398"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(15..308)
                     /locus_tag="PC407_003752"
     CDS             complement(15..308)
                     /locus_tag="PC407_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499119.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
BASE COUNT          157 a           77 c           24 g          158 t
ORIGIN      
        1 tattaattta tactttaaag attcccatct ttatcatatt ttattcttat aagaaaatat
       61 aatcctatta ctactaactc tatcactaaa aatactccat aggtaagaat tgaatcaata
      121 ctatctaacc catttctaaa aaaaattaat gctatacctg ctgctataat ccctatagca
      181 ttttttctta ctgcattttg cccaactcca taaaaacctg gcatttttcc ttttatttcg
      241 acagcagtta aaattatccc aaacactata aataatactc caataagcat cataatcata
      301 ctaatcatct aattacctcc ttcacttaag gaaatttcaa tttctaaaat ctaaaataaa
      361 taatatttat tttttataat aatattgtat atccaattaa ctattttgga aattag
//
LOCUS       DAMJVX010000388          387 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.399, whole genome shotgun sequence.
ACCESSION   DAMJVX010000388 DAMJVX010000000
VERSION     DAMJVX010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.399"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            47..241
                     /locus_tag="PC407_003753"
     CDS             47..241
                     /locus_tag="PC407_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2499120.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYEM
                     TDAYRFETLCSDEKIRFKRI"
BASE COUNT          152 a           28 c           54 g          153 t
ORIGIN      
        1 ttattatata tttggatatt ttcatattaa taggagggat tctacaatga taaattatat
       61 ggtagaaatt atatatagcg aaaaaataag cgaagtagaa aacatgttga aaattgaaaa
      121 tatacaatat gaatttactt ctcgtgatgg ctttatattt tttggttttt atgagatgac
      181 agatgcatat agatttgaga ctttatgttc agatgaaaaa attcgattta aaagaatata
      241 ataaataaaa aaagtacata tttctaaact atttgttttt caaatttagg ttgtgtttta
      301 atgtagtgtt gatatcataa acaattttaa taataaaaaa gtaggaatat tatattaata
      361 tgctcctact ttttactcat atttatt
//
LOCUS       DAMJVX010000389          380 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.400, whole genome shotgun sequence.
ACCESSION   DAMJVX010000389 DAMJVX010000000
VERSION     DAMJVX010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.400"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
BASE COUNT          140 a           48 c           28 g          164 t
ORIGIN      
        1 attatttttt attaattaac tttattttac atatatataa aaaaactgca aataaaaatt
       61 aacttttatt tacagttctc acttatttat ataattcaaa accttattgg ttatcattta
      121 cagtgtcttc taatatattg ctttctgata tatatgatag aattgaaagt ccatacataa
      181 ccaaaactaa tgctgctgtt ataagtacta ttcccatatt taatacctcc ttttgtttac
      241 agtatactat tattatgtga aatatttatg aatttaattt gaatatatat gtaatttcac
      301 actaacaatt tcttcaaatt aaacattatt aactatatac ttttgttgaa ccaattatga
      361 ttaatagttt aaatattagc
//
LOCUS       DAMJVX010000390          376 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.401, whole genome shotgun sequence.
ACCESSION   DAMJVX010000390 DAMJVX010000000
VERSION     DAMJVX010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.401"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            132..320
                     /locus_tag="PC407_003754"
     CDS             132..320
                     /locus_tag="PC407_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499121.1"
                     /translation="MNYHTKEELMEVLRVASSRIINCEKVQKKFSEETSHHTRFKNII
                     EAMYISKSLIMDEISKRD"
BASE COUNT          160 a           27 c           60 g          129 t
ORIGIN      
        1 gtaatgtcac tgatagttag aatatttaag tgttaaaaga tatatataaa taataatgat
       61 atcagttgat aatgatatta aattgaggta tactgtttct ctaaattaaa aaacaaagga
      121 gtaaatgtgt tatgaattat catacaaaag aagagttgat ggaagtactg agagtagcat
      181 cttcaaggat tattaattgt gaaaaagtac agaagaaatt ttctgaggag acatctcatc
      241 atacacgttt taagaatatt atagaagcaa tgtatatttc aaaatcatta ataatggatg
      301 aaattagtaa aagagattga agcttatttt gcaatttaat atgttttaat atacatttta
      361 ttatataaga aaataa
//
LOCUS       DAMJVX010000391          372 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.402, whole genome shotgun sequence.
ACCESSION   DAMJVX010000391 DAMJVX010000000
VERSION     DAMJVX010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.402"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(146..346)
                     /locus_tag="PC407_003755"
     CDS             complement(146..346)
                     /locus_tag="PC407_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2499122.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
BASE COUNT          124 a           58 c           35 g          155 t
ORIGIN      
        1 atttttgtca gatttatttt ataaaaagta tttagactaa gtattatttt taagtatttt
       61 cttgttgaag ttattttttc cttttttaca aaatgcgtaa ataaaaaaaa gactcttaaa
      121 agagtctttc cgaaaaatct atatattata atatagttat attttctgct tgaggacctc
      181 tagcgccttt aactatatca aagcttactt tttgaccttc ttctaatgat ttaaatcctg
      241 aagtttgtat agctgagaaa tgagcaaata cgtcatctcc actttccatt gatataaatc
      301 caaatccttt ttcattatta aaccatttta ctattccatt attcattaaa aatactccta
      361 ttaacctata tc
//
LOCUS       DAMJVX010000392          364 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.403, whole genome shotgun sequence.
ACCESSION   DAMJVX010000392 DAMJVX010000000
VERSION     DAMJVX010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.403"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="PC407_003756"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="PC407_003756"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            291..362
                     /locus_tag="PC407_003757"
     tRNA            291..362
                     /locus_tag="PC407_003757"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Asn,seq:gtt)
BASE COUNT          117 a           61 c           78 g          108 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc tatgtaaagt actaacttca
       61 attgatgaag tatatatcgt aaaaacaatt actatttagt acttcaaaac tattgacatt
      121 taaaaataaa gatgttaata taatacttgt cttataaata ggcaatatgt ggttataata
      181 gcaaagagga tacacctgtt cccattccga acacagaagt taagctcttt agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtgaatat tccaaggtag
      301 ctcaatggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc ccacccttgg
      361 agcc
//
LOCUS       DAMJVX010000393          350 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.404, whole genome shotgun sequence.
ACCESSION   DAMJVX010000393 DAMJVX010000000
VERSION     DAMJVX010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.404"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(<1..>350)
                     /locus_tag="PC407_003758"
     CDS             complement(<1..>350)
                     /locus_tag="PC407_003758"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDF2499123.1"
                     /translation="NVAKDLFNGEIVAYEISTKMDTKLVNKTVNQLINMNLAKDCILH
                     TDQGSQYTSRSYSKRLKDNGIIQSMSRRGNCWDNAPIESFFSHFKSELIYLIDTTDPK
                     EMISLINDYIYFYN"
BASE COUNT          117 a           52 c           38 g          143 t
ORIGIN      
        1 ttattataaa aataaatata atcatttatt agactaatca tttcctttgg atctgtagta
       61 tcaattaaat atattaattc agatttaaaa tgactgaaaa aactttctat aggagcatta
      121 tcccagcagt tgccccttcg ggacatagat tgtataatac cattatcttt aagcctttta
      181 gagtaagatc tacttgtata ttgtgaacct tgatctgtat gtaaaataca atctttagct
      241 aaattcatat ttattagttg atttactgtt ttattaacta atttagtatc catttttgta
      301 cttatctcat atgccactat ttcaccatta aataaatctt tggctacatt
//
LOCUS       DAMJVX010000394          347 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.405, whole genome shotgun sequence.
ACCESSION   DAMJVX010000394 DAMJVX010000000
VERSION     DAMJVX010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.405"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
BASE COUNT          136 a           37 c           29 g          145 t
ORIGIN      
        1 aattttattg attctacctt tttaaataac tgtcgattta ttaaagttat ctatgtacta
       61 tattaaacaa ttaagtagtt attaattgca cttattagtt tattaaaaaa ctatgtatat
      121 tactttgcag taagactttg gaactaaatc tataatagca aatttaaatt tattaaatag
      181 attaccttat aacatttcat ataaaatata ttgtgaatct gagaaaatta ctatttttct
      241 ttgagtattt tttataagca gttctaaaag attcaatttc atacctatta cttgaagcat
      301 tcattaaaat aattctaatt taaaaaataa ttctatccaa ataatag
//
LOCUS       DAMJVX010000395          347 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.406, whole genome shotgun sequence.
ACCESSION   DAMJVX010000395 DAMJVX010000000
VERSION     DAMJVX010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.406"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
BASE COUNT          156 a           30 c           34 g          127 t
ORIGIN      
        1 gtcattaaat atgccaatag ttttatatat tattgatttt attaaaaaaa gagatataca
       61 aactaataag ttgtaatctc tcaaatataa aaaaacaata aattaactca ttggattttt
      121 tagtccataa tataattata gattattcaa caaacaatct atagaaattg tggtattaat
      181 gtttaaataa ccaacttttt ctaaaataac ctttatagga atatatgagt tcataagtaa
      241 agatagcgaa ataagaaacc agtttggtat gtaaaatcta gatttttaat taaattatca
      301 agattatata ttatagtatc aaaaatagaa aattaaaatt aaatttt
//
LOCUS       DAMJVX010000396          343 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.407, whole genome shotgun sequence.
ACCESSION   DAMJVX010000396 DAMJVX010000000
VERSION     DAMJVX010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.407"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..>343
                     /locus_tag="PC407_003759"
     rRNA            <1..>343
                     /locus_tag="PC407_003759"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           81 a           76 c           98 g           88 t
ORIGIN      
        1 tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa
       61 cccttgtctt tagttgccat cattaagttg ggcactctag agagactgcc agggataacc
      121 tggaggaagg tggggatgac gtcaaatcat catgcccctt atgcttaggg ctacacacgt
      181 gctacaatgg gtagtacaga gggttgccaa gccgtaaggt ggagctaatc ccttaaagct
      241 actctcagtt cggattgtag gctgaaactc gcctacatga agctggagtt actagtaatc
      301 gcagatcaga atgctgcggt gaatgcgttc ccgggtcttg tac
//
LOCUS       DAMJVX010000397          314 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.408, whole genome shotgun sequence.
ACCESSION   DAMJVX010000397 DAMJVX010000000
VERSION     DAMJVX010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.408"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(<1..146)
                     /locus_tag="PC407_003760"
     CDS             complement(<1..146)
                     /locus_tag="PC407_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDF2499124.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGI"
BASE COUNT          125 a           52 c           25 g          112 t
ORIGIN      
        1 tagattccta ccattccatc agacccaaat aacatcccta gtaagataaa aatcaataat
       61 attggtactc caaatttata caataccttg ctagaagtta tacaaatcaa tagtactaac
      121 ccacttataa tcattaattc tgtcatagca tcaactccat ttcatataaa ttttgtagat
      181 taatttgcat ctacaatttt attacttatt caaatataac atgacataaa ttttataagt
      241 tatggtacat aaagttcaac aaaaatttca caatacatta aaatgtgagt gttatactta
      301 aatttatata tttg
//
LOCUS       DAMJVX010000398          306 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.409, whole genome shotgun sequence.
ACCESSION   DAMJVX010000398 DAMJVX010000000
VERSION     DAMJVX010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.409"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
BASE COUNT          114 a           31 c           27 g          134 t
ORIGIN      
        1 aaaattaata tggataaata ttctaaaccc agtatttatt aaaaatgaaa taaaaagaaa
       61 aaaaatacaa attattatct ttttctttat aaactttggt ttaacttatt cattaggttt
      121 actgttatat tataataaat ttattgatcc agaaaacttt gcatcattca tgatgatatt
      181 acctctatct tcagttgcta ttgcaaagtt ttatactgaa ggattaaata atgatatgta
      241 tgaattttat tcactaatca tattattttt tcttatttat tcattattaa ttgttcttaa
      301 gttatt
//
LOCUS       DAMJVX010000399          306 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.410, whole genome shotgun sequence.
ACCESSION   DAMJVX010000399 DAMJVX010000000
VERSION     DAMJVX010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.410"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(<1..291)
                     /locus_tag="PC407_003761"
     CDS             complement(<1..291)
                     /locus_tag="PC407_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2499125.1"
                     /translation="MSADGSYTMGASIVAFSLTNGISPVVATLMAILCGCTAGLVTGI
                     LHIKFKISNLLSGILVMGMLYSINLRIMGKSNIPLFSFKHLFNGEISPIVLAL"
BASE COUNT          123 a           52 c           49 g           82 t
ORIGIN      
        1 taaggctaaa actataggag atatttcccc attgaataag tgtttaaagc taaatagagg
       61 aatatttgat tttcccatta ttcttaaatt gatagagtat agcattccca taactaaaat
      121 accagaaagt aagttagata tcttaaactt aatgtgaaga atccctgtaa caagaccagc
      181 agtacaacca cataagatag ccattaaagt tgctactaca ggagaaatac cattagtaag
      241 agaaaatgct acaatagaag cacccattgt atagctacca tcagcagaca tgtctggaaa
      301 atctag
//
LOCUS       DAMJVX010000400          283 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.411, whole genome shotgun sequence.
ACCESSION   DAMJVX010000400 DAMJVX010000000
VERSION     DAMJVX010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.411"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            26..232
                     /locus_tag="PC407_003762"
     CDS             26..232
                     /locus_tag="PC407_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499126.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFNKID"
BASE COUNT          130 a           16 c           41 g           96 t
ORIGIN      
        1 ataattttaa aaggagtgat ataatatgag taaatatagt tctttatggg aatatgttaa
       61 gaaaaataat agtcaatcgt ttaaattaac ttttgaagaa ataaaagata ttgctggaat
      121 agagatagac cattcgtttt tgaaatataa aaaagaatta aatgaatatg gatatcaagt
      181 aggaaaaatc tctttaaaag agaagactgt tattttcaat aaaattgatt aagatataaa
      241 taattacata taataaaata atttgtattg acgattatta caa
//
LOCUS       DAMJVX010000401          277 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.412, whole genome shotgun sequence.
ACCESSION   DAMJVX010000401 DAMJVX010000000
VERSION     DAMJVX010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.412"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
BASE COUNT           91 a           41 c           18 g          127 t
ORIGIN      
        1 tctataaaaa tcatactatt ttagctgttt acttgcttat ttgatagttt tatttctttg
       61 attaaatatc tttcagaatc taagccagat gtctcaaaaa aactgattta taatataatc
      121 attttttctg ctattcttat ttttccaatt ttagtttcat attaaaatat acttttcata
      181 gacactacat tctttcatac tcattttcac atttaactat acaagtagtc ttactcattt
      241 atgtactata ctttattaat atttattaag aaaaact
//
LOCUS       DAMJVX010000402          206 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.denovo.413, whole genome shotgun sequence.
ACCESSION   DAMJVX010000402 DAMJVX010000000
VERSION     DAMJVX010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.denovo.413"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(<1..167)
                     /locus_tag="PC407_003763"
     rRNA            complement(<1..167)
                     /locus_tag="PC407_003763"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           52 a           55 c           39 g           60 t
ORIGIN      
        1 ttatcccgta ttagcatacc tttcggtatg ttatccgtgt gtacagggta ggttacccac
       61 gcgttactca cccgtccgcc gctctttacc gaagtaaatc gctcaacttg catgtgttag
      121 gcacgccgcc agcgttcatc ctgagccagg atcaaactct caaataaaag tttatcaggc
      181 tcagatacta ttgttatcaa aatatc
//
LOCUS       DAMJVX010000403        54683 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.guided.010, whole genome shotgun sequence.
ACCESSION   DAMJVX010000403 DAMJVX010000000
VERSION     DAMJVX010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54683
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.guided.010"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            complement(116..808)
                     /locus_tag="PC407_003764"
     CDS             complement(116..808)
                     /locus_tag="PC407_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2499127.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMMAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(834..2021)
                     /locus_tag="PC407_003765"
     CDS             complement(834..2021)
                     /locus_tag="PC407_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2499128.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRIPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSISYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            2357..4234
                     /locus_tag="PC407_003766"
     CDS             2357..4234
                     /locus_tag="PC407_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2499129.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKTVYDEVIKIVPQSNDVGKKNESQEKQSIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSFIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            4546..5952
                     /locus_tag="PC407_003767"
     CDS             4546..5952
                     /locus_tag="PC407_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2499130.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNTVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            5980..6828
                     /locus_tag="PC407_003768"
     CDS             5980..6828
                     /locus_tag="PC407_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2499131.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            7489..8181
                     /locus_tag="PC407_003769"
     CDS             7489..8181
                     /locus_tag="PC407_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2499132.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            8178..9113
                     /locus_tag="PC407_003770"
     CDS             8178..9113
                     /locus_tag="PC407_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2499133.1"
                     /translation="MKMDEQTIILFLFLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVDDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTVFTVSLPLI"
     gene            9261..10184
                     /locus_tag="PC407_003771"
     CDS             9261..10184
                     /locus_tag="PC407_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2499134.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAHAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            10168..10938
                     /locus_tag="PC407_003772"
     CDS             10168..10938
                     /locus_tag="PC407_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2499135.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVTVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            10948..11730
                     /locus_tag="PC407_003773"
     CDS             10948..11730
                     /locus_tag="PC407_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2499136.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGIAVLLSLFSFLCTFILGLLFCNINISVTLIVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            12107..12643
                     /locus_tag="PC407_003774"
     CDS             12107..12643
                     /locus_tag="PC407_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2499137.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            12675..13064
                     /locus_tag="PC407_003775"
     CDS             12675..13064
                     /locus_tag="PC407_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2499138.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            13101..13487
                     /locus_tag="PC407_003776"
     CDS             13101..13487
                     /locus_tag="PC407_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2499139.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGSQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            13614..15056
                     /locus_tag="PC407_003777"
     CDS             13614..15056
                     /locus_tag="PC407_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2499140.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVENESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLIDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            15353..16168
                     /locus_tag="PC407_003778"
     CDS             15353..16168
                     /locus_tag="PC407_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2499141.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINNFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKRVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
     gene            16769..17953
                     /locus_tag="PC407_003779"
     CDS             16769..17953
                     /locus_tag="PC407_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499142.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(18330..19439)
                     /locus_tag="PC407_003780"
     CDS             complement(18330..19439)
                     /locus_tag="PC407_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2499143.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            19902..21095
                     /gene="nifV"
                     /locus_tag="PC407_003781"
     CDS             19902..21095
                     /gene="nifV"
                     /locus_tag="PC407_003781"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HDF2499144.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVI
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            21120..23045
                     /locus_tag="PC407_003782"
     CDS             21120..23045
                     /locus_tag="PC407_003782"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HDF2499145.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSNVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            23067..24062
                     /locus_tag="PC407_003783"
     CDS             23067..24062
                     /locus_tag="PC407_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HDF2499146.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGAEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            24389..24862
                     /locus_tag="PC407_003784"
     CDS             24389..24862
                     /locus_tag="PC407_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2499147.1"
                     /translation="MVGSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            24896..25390
                     /locus_tag="PC407_003785"
     CDS             24896..25390
                     /locus_tag="PC407_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2499148.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            25487..26008
                     /locus_tag="PC407_003786"
     CDS             25487..26008
                     /locus_tag="PC407_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2499149.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMYDDNIEADKYIREELKVPSKYSVECIISIGYSDEEKKAYND
                     SDLDYKKVHFNNF"
     gene            26197..26508
                     /locus_tag="PC407_003787"
     CDS             26197..26508
                     /locus_tag="PC407_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2499150.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            26536..27222
                     /locus_tag="PC407_003788"
     CDS             26536..27222
                     /locus_tag="PC407_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2499151.1"
                     /translation="MKYSYKKCIIDSILLMFIIQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            27454..28338
                     /locus_tag="PC407_003789"
     CDS             27454..28338
                     /locus_tag="PC407_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2499152.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            28437..29612
                     /locus_tag="PC407_003790"
     CDS             28437..29612
                     /locus_tag="PC407_003790"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HDF2499153.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEELLKDIPTANKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            29740..30594
                     /locus_tag="PC407_003791"
     CDS             29740..30594
                     /locus_tag="PC407_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499154.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSNESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKIKDDSSC
                     LYLDIYND"
     gene            30638..31873
                     /locus_tag="PC407_003792"
     CDS             30638..31873
                     /locus_tag="PC407_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDF2499155.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREIVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKSISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYISLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNEKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            31974..32915
                     /locus_tag="PC407_003793"
     CDS             31974..32915
                     /locus_tag="PC407_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HDF2499156.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(33144..33497)
                     /locus_tag="PC407_003794"
     CDS             complement(33144..33497)
                     /locus_tag="PC407_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2499157.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMIW
                     LEKLKEDNTYEIYSI"
     gene            33793..34704
                     /locus_tag="PC407_003795"
     CDS             33793..34704
                     /locus_tag="PC407_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HDF2499158.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            34964..35398
                     /locus_tag="PC407_003796"
     CDS             34964..35398
                     /locus_tag="PC407_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499159.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            35421..36956
                     /locus_tag="PC407_003797"
     CDS             35421..36956
                     /locus_tag="PC407_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499160.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVIYK
                     IDCDVILVNLFELIINNCIFSTILGNEVINLEISKYEYEFLEKQFKNMYNNITEDKVI
                     GSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKTEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            37040..38407
                     /locus_tag="PC407_003798"
     CDS             37040..38407
                     /locus_tag="PC407_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2499161.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKARDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMNTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            38442..38666
                     /locus_tag="PC407_003799"
     CDS             38442..38666
                     /locus_tag="PC407_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDF2499162.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(39116..39514)
                     /locus_tag="PC407_003800"
     CDS             complement(39116..39514)
                     /locus_tag="PC407_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HDF2499163.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMVG"
     gene            complement(39517..39918)
                     /locus_tag="PC407_003801"
     CDS             complement(39517..39918)
                     /locus_tag="PC407_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2499164.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLVAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            40277..41263
                     /locus_tag="PC407_003802"
     CDS             40277..41263
                     /locus_tag="PC407_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2499165.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLNEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            41247..42158
                     /locus_tag="PC407_003803"
     CDS             41247..42158
                     /locus_tag="PC407_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2499166.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            42271..43839
                     /locus_tag="PC407_003804"
     CDS             42271..43839
                     /locus_tag="PC407_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2499167.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHVLEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            43914..44939
                     /locus_tag="PC407_003805"
     CDS             43914..44939
                     /locus_tag="PC407_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2499168.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDEYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            44932..45876
                     /locus_tag="PC407_003806"
     CDS             44932..45876
                     /locus_tag="PC407_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2499169.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            46235..47893
                     /locus_tag="PC407_003807"
     CDS             46235..47893
                     /locus_tag="PC407_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2499170.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERDSKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASNGKSSW"
     gene            47874..48890
                     /locus_tag="PC407_003808"
     CDS             47874..48890
                     /locus_tag="PC407_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2499171.1"
                     /translation="MVKAVGDIIGKCSAISFDMKLDKSYDEVIVDFKNLINNIDNKDV
                     LLFTDMGSLNSFDEIIKKEKKCEVRVIPMVTTLTVLEAVQKANMGLPLNDVYNSITNT
                     RKYYFGTNEIQNKENLSKTIIIASHVSEGVDNKTRKILEEKMSRYLDGIDIISVPYKT
                     EKDLSLNITKLKESSNIVAVINEQRINIRGIDYISKKDIDKDENINKLKNIIKISIGY
                     DDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLVFNIEKKYDKVIGLMMHLAFMVDGLI
                     GNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDKKYNIEINEKECYQILLILEYAEIIE
                     KDYQ"
     gene            49105..49734
                     /locus_tag="PC407_003809"
     CDS             49105..49734
                     /locus_tag="PC407_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499172.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            49869..50195
                     /locus_tag="PC407_003810"
     CDS             49869..50195
                     /locus_tag="PC407_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2499173.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            50211..51500
                     /locus_tag="PC407_003811"
     CDS             50211..51500
                     /locus_tag="PC407_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2499174.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            51524..51853
                     /locus_tag="PC407_003812"
     CDS             51524..51853
                     /locus_tag="PC407_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2499175.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            52001..53329
                     /locus_tag="PC407_003813"
     CDS             52001..53329
                     /locus_tag="PC407_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2499176.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            53646..54506
                     /locus_tag="PC407_003814"
     CDS             53646..54506
                     /locus_tag="PC407_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDF2499177.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
BASE COUNT        21103 a         6008 c         9805 g        17767 t
ORIGIN      
        1 tgaaatgatg aaaatatatt tgatttcata caaaaaagag tgcctcataa ctaaaaagtt
       61 cactttgaga cactccattt ttatatgtta gttgcattta aggtaaaata gaagtttaat
      121 catgttgaaa taactgaatt acatcttcta aatgattgta cattgatgta taacctaagt
      181 aagagtcttt actatcaaat gccatcatta tcttgttatg gtggctaatc atatccttaa
      241 gagaatttct atatttaaaa ccgaagtatt ggttagaaga aactgtttta ggtattaact
      301 cataatattt tataaaaatt ggattatgag aagctaaaat tatctctttg tgaaattgta
      361 agtcagcatc ataacattct tctatcaaac catgagaaac cttatccaat tcctctattg
      421 actttaggta agtatatttt attttttgaa gttctttaga tgtagcattt aaaattgcta
      481 gtttagtagc ttcactttcg ataagttgtc ttgctcttaa agattctaca taaaattctt
      541 gtatgttatg atttttatag atttcaccat catcaaatag gtcaactttt ttaaatcctt
      601 caactatagg acttgagtaa ggcacagctc taagtattcc ttccttacag agtatattta
      661 tggcatttct tacaggtatt ctacttatgt taaataggtc agcaatcttt ctctcacttg
      721 gtaatttact attaatttta aaagttccat ttagaatgtt taatttaata taatcaataa
      781 ttttttcagt ggcatttaag cttttcataa aaactcctaa acatataatt tttttatgat
      841 tgtattatat catagttaag agttaaatct acttttttat aatatgtata agacaggata
      901 gaggccaaaa tagctataat agccaagata aagtatccat attgtattga agtatattcc
      961 gatattttac caactatcat tggaaataca gacattccta tagcataaat tgcttgaaaa
     1021 aaacccattg cagtagattt ctgatgtttt ggtacatttt tcatagactc gctagtaagg
     1081 taagaaagga gtatacctgt agatatacct ggtagcactt gtagtacaaa tattatatta
     1141 atatttttta ttgttggaac taaaaagcaa taggctgaaa ctaaaataaa aactaaagga
     1201 atccaaaatt ttggcccata cttagtacaa aatttagtag aagcaaagcc agaagaagca
     1261 acagcacaca ccatgtaaga tatagaagat agacctatga gggtagaact agaacctaag
     1321 tttttaagaa tttgagtagt aaatgacatt gttgtagaca tttgaatacc ctgttgaatt
     1381 aatgccataa aggaaaaaaa tacaagtctt ttatctttgc agatagataa atatttttta
     1441 attgatattt tttcaggtat tttttttgaa ttatgatttt ttatatttaa ggctagaatc
     1501 gttccaataa ctgaagacaa tacactcatg atacataagg ctgtcattcc taatttttca
     1561 taaaataaag tactcataac aaatccaatc aacattccta gattgtttac caatattatc
     1621 gtactcgtag ctttttgttg atgttctgta gaaaaaaagt tggtatagtg aaccatgtaa
     1681 gaaatccaag ttgatgatga tattccagat aaaatgttag ctataagaaa accattgcct
     1741 ccaggagaaa agactcttat aagagatgct aaaccagcaa agaaaattcc agacagtata
     1801 aatattttat gtttatttat agaatcagca aatactccaa caggaatcct taataaacat
     1861 tgtgagattc catatgcacc tattattata ccaatcatat ttgtagatac attacatgca
     1921 cttaaatatg gattttggaa aggtatatat acatattgtg aaaaccagaa aaatgtaaca
     1981 atagaaagta aaagtaattt ttgattagat atttttttca tattatcctc catatggtat
     2041 ttttataatg caaataatat accatttaga ttttttaatc aagtactttc tctattaaaa
     2101 aaaatttaaa tatataatta ttatttacaa aaaaattata ctttatagtt gaattaagaa
     2161 aacgcttatg atactataaa gttaacaaat ttgaatatat aacagtagac gtgttactgg
     2221 taatgcaggc aagatcgaat caatttttat agattatcta tagagtacta tgggtataat
     2281 ctatttattt ggttcgattt ttttgtttaa ttggccataa aacaaaaagc ctatagttga
     2341 aaatggagga ttttttatgg attataaaaa tatagctcaa gaaattctac taaatgtagg
     2401 aggaaaagag aatgtaaatg aagtgactca ttgtatgaca agacttagat ttaaagtcaa
     2461 aagcgcttct aaagtgaata aggataaatt atctaaaact gaaggagtaa taactgttgt
     2521 tgagagtatg ggtcaaatac aagttgtaat tggaaataaa gtaaaaacag tatatgatga
     2581 ggttataaaa attgtacctc aatcaaatga cgtaggtaaa aagaatgaaa gtcaagaaaa
     2641 gcaaagtata attaattcta tattatcagc agtagctggt atatttacac ctacaatacc
     2701 tgctatagca ggggttggta tgattaaagg tattttatca gttcttgcta tgtattatat
     2761 gaataaaaat ggagtagata taaaagaaac gcaaagttat attatcttaa atgccatggc
     2821 cgattctata ttctatttta tgcctattat actaggatat actgcggcta aagtatttaa
     2881 tgcaaataaa ataatatcta tggtattagg agctacactt tgttatccaa cttttactgc
     2941 tttgatggca ggtgaagaat ctgttagatt tttaggattg gcagtaacaa aagctacgta
     3001 tacatcatct gttattccaa taattattgc tatatgggct ctttcatacg ttgaaaaagt
     3061 attagaaaag tatatacctg aggtaattaa aataataatg gttccaactt tatcattggt
     3121 tgtaatgtta cctgcaacat tatttttatt tggaccaatc ggaatttata taggaaatgt
     3181 cataaacttt tcttataagt atatttatga attaagccca gcgttgtgtg gagcatttgt
     3241 aggtgggtta tggtgtgtgt tggttatatt tggtgctcat agagcattat taccaatagg
     3301 tataagtgat gttgctcaaa caggaagaca gaatttattg gcatttgcag gagctgcaaa
     3361 cttttcacaa gcaggggctg ctctaggagt attctttaaa actaaaaatc aaggactaaa
     3421 aacaatttct atgtctgcaa ctataacagc tttatttggt ataactgaac ctgcaatata
     3481 cggtgccaat cttagattaa aaaaacctat ggtatgtgcc gtaatatgtg gtgcatttgg
     3541 aggtgcgata atgggaatgg gtggagctta tggtaatgca tttgcaaacc aaggtgtttt
     3601 aactatacct gtatatgctg aagcaggtgc attaggtttc ttaagttatc taggtggttg
     3661 tgctatagca ttttttggtt cagctatttc aacttatcta gttggatttg aagatttgga
     3721 agagtcttct aataaggaaa attcatttat aaaagtagaa acgaaggatg gtgttattga
     3781 tataacttca cctgtagagg gcgaatgtat agaattgtca gaagtaaagg atgatgtatt
     3841 tgcttcaaaa gctatgggag aaggtatagc tgtgttacct acaaaaggag taataacagc
     3901 ccctactgat tgtgaagtag cttctttatt tccaacatta catgcaatag gattaaaatt
     3961 agataatgga gcagaaatgt taatacatgt tggaatcaat acagtagagt taaatggtaa
     4021 atactttaca aaacatgtta atcaaggaga tttagtgaaa aaaggtgata aacttatatc
     4081 gtttgatata gataaaataa aaaaggctgg ttatgatgta actacgccag ttatagtaaa
     4141 caatacattt gattttgggc aagtagtttc ttgtaaatct agttatgttt caaccaatga
     4201 taatataatt tcattagtat tagctggtaa ttaagttaat ttgactagag ataagtaaat
     4261 tgatttaaaa ggaaataagt aaatcaacaa ttaaatacat ctaaaagtaa gtttattgca
     4321 atgaattgtt aaagaaaata attaatatgg agtaaagcta ttgcaatgaa gtaacaaaga
     4381 aaataattaa tatagagtaa gtttattgta atgaattgtt aaagaaaata attaatatag
     4441 ggtaaatcta tctcaatgaa ataacaaaga aaagagttaa tatagagtga acttattgca
     4501 atgaatcaac aaaaaaaata atcaatataa aaaggagaaa tatttatgaa tacaggattt
     4561 ccaaaagact ttttatgggg agcatcatct tctgcatttc aagtggaagg agcttgggat
     4621 aaagacaata aaggaaaaac cgtagccgac tataattctt ttaaaaagtc tcatttacag
     4681 gcagatacta aagttgcatc agacttttac cataattatg aagaagatat tgaattaatg
     4741 aaagaattag ggatgaaaac atatcgtttt tcaatttcat gggcaaggat aataccagat
     4801 ggtgaaggag agataaacca gaaaggttta gacttttata ataagataat agacaaacta
     4861 atagagtgtg atattgagcc attcgtaact ttataccatt ttgaccttcc atttaaatta
     4921 gtagagaaat ataatggttg ggaaagtaga gaaactgtct atgcttttga aagatttgct
     4981 aaaatatgct ttaagcattt tggagatagg gtaaagtatt ggcaacctca taatgaacag
     5041 aatttaattg ttagagtgga ggagagaatt aatatatatg atgaaacaga ttcatggaag
     5101 atagataaaa tgcgtgctca gatggattat aatctttgct tagcacatgc attagcagtc
     5161 aatgcatgtc atgagatgat aaaagaaagt aaaattggag cagcagtatc atcatctgta
     5221 acatatcctc ttacttctaa gccagaagat gtatatgcag ctagaatgaa tgataatttt
     5281 aaagtgtact atatgcttga tatgcatcat tatggagaat atccagggta ttatatgaag
     5341 tatcttgaaa aaagaaatac agttcctcac atggaagatg gggataaaga gatacttaaa
     5401 aaagctaaaa tggattttat agctgtaaat tattatagga caaattgtgc agaagcttta
     5461 cctgaagatt cacaacatcc atttggatta agagaaggaa ctgttgattt tagtatgtat
     5521 ggactattta aaatgtcaat gaatcctaac ttagaggctt ctgagtatgg agcagcaata
     5581 gacccatcag gtcttagagt tgccttaaat gaatattggc aaagatatca cttacctgtt
     5641 ataataacag aaaatggtct aggagctaaa gatatattag aagatggaaa aattcatgat
     5701 gactatcgta tagactattt aagaagtcat ataaatgcat gtaaactagc tattgaagat
     5761 ggtgtagaga tgattggata ttgtccatgg tcatttacag accttttaag ttcttctcaa
     5821 ggttttaata agcgttatgg acttgtttat ataaatagaa ctgaccatga agttttagat
     5881 ttaaaaagaa tcaagaaaga tagtttttac tggtataaag aagttataga gaacaatgga
     5941 atagtaaaat aaacataaat atatattgag ggttttagta tgtacattac aaaggtatta
     6001 aataattcgt tgctacttgc aaaggatgat aatgatgaag aaataatatt gatggggaaa
     6061 ggcattggcc ataattataa ggccgggtct gagttaaata aaaaagatat agaaaagatt
     6121 tttgttcttc atgatgaaaa tataaaaaag agtattatac aattggcaac agaaatagat
     6181 gcagaatatt ttggtatagc tcaaatgata attgcatatg gaatagaaaa atataactta
     6241 aaacttatga accatatata tttggcctta actgaccaca ttgcctttgc agtgagacgc
     6301 tttaaagaag gtataatgat tgaaaatcac tatttgttcg aaatcaagga atttaatcca
     6361 aaagagtatg atataggaaa atatgctatt actgttttta aggaggttct tggtttagac
     6421 ctaccagaag aagagattgg taatatagca ggccatttta taaatgcaca acaagataat
     6481 ccttatagtg atagaaataa gagaagtgct aaaattgtaa atgctatttt gcaaatagtt
     6541 cattatcact tttctattgt atataataaa gagtcatttt attataggag atttgtaatg
     6601 catctaaagg ccttttcaca aagatttctg gctaatgaac catcaagaga aaaaatagat
     6661 tttatatatg agcaagttca aaaaaattgt aaggaagagt atgaatgtgt gaaaaaggta
     6721 ggagcttata tgtataagga atactccaga gaattaccaa gacaagagga gttatatttg
     6781 atgattcata ttcataagat tttaggagaa ttatgcgaaa atagttagaa tatcttaata
     6841 tgctagtaat attataaaaa attaaaaatt aactacactt taaatatgaa caattgcatt
     6901 acttaagggg gttgtcttaa aattgatttt tttaattaaa tttgaagctc aactcctttt
     6961 ttaaaccttt atatcattac aactttaatg tttatacgcc tgtactttag atgtatatct
     7021 actttttaca tttctcaaca tgcaaaccta ccattttttg cagtatgtta attgaaataa
     7081 accattgagt taaccctaaa tttattctgg acttatagga atagttccaa agatacttta
     7141 aaatattttc tggaataatt gcttatttca catatgtttt attgtgatgt tttgatttaa
     7201 taaattcttc aactctgtat atagtaaatg tattatcatg tttgttttat tcaagcacac
     7261 ttataatacg cttgattttt tcacaatgat gaaatatact aatgggtgtt ataggtataa
     7321 gaatcgaatt acacttgaag aagtaagtga taaatgagat ataaatcctc atatgataaa
     7381 ctactatttc attgaggaac atattctaag tgatgtcaag atactacaat atgactaaat
     7441 tcctagtgat aaaaaagtct acgaatgagt gtagaaaggg gaactaagat gaataaagtt
     7501 ttaattatag atgatgataa agatctttgt attttgttaa aaaagagtat ttcaattgaa
     7561 cagattgaag caaactattg ccattctggg agagatggac tggttgaact ttcaaaacaa
     7621 gattatcagt tagtagttct agacgtgatg atgccaggta tggatggttt tgaaactatg
     7681 gaacaaatac gaaaatatag cagtattccc atcttaatgc tgacatcaaa aagcgataat
     7741 tacagcaaag tgcatggatt gcgagctggt gcagatgact atctaaccaa accatttgaa
     7801 attacagaat ttactgctcg tgtactgtct ttaatccgac gttacacccg ttttaatcca
     7861 aaaaatgagg aaccacagca actttctttc aaaggtttgt tgattgacct tgaccagcat
     7921 acagtgacta ccaatggcga acctatgttg cttccaccca aggaatttgg tgtgcttctt
     7981 tactgtgcaa ggcatcaggg taagattttg acaaaacagc agatttatga agctgtgtgg
     8041 ggtgagccat atgtatatga tgacagcaat atcatggcta ttatcagtcg cctaagaaaa
     8101 aaaattgaac tagacccatc atctcccatc tatttgcaaa caataaaagg aattgggtat
     8161 caattcaaca cggaggtatg aaaatggatg aacaaacgat aattcttttc ttattcttgc
     8221 tgtcgattgg tctacttttt ctttccatat ttgctttttc caagcttggg catattcaca
     8281 aacgcctaaa agatatagag gagatactgg ctgatgtaca aattggaaac gaaaatcgca
     8341 aaatactaat taagccatgt gatgttatgg ctccattggt ttatcagctt aatgagattg
     8401 tctacgatta tgaaaataag ttactttctc tgaaaaagtc ggataaagct agcaaacaac
     8461 ttatgacaag tctatctcat gatgttcgta caccattaac aactctgatt ggttatctgg
     8521 atgctgtaca cagtggtata gttatcggac aagaacggga ggaatacctt gaaatcgcta
     8581 ggcgtagagc ctacgacctc aaggattaca ttgatgttct ttttgactgg tttcggttga
     8641 actctgatga atttacgctg tcaatagaat ctgtggaaat tgcagagttg tcacgcaata
     8701 ttttgaaaga ctggattcct atattccatg aaaagaaact gaattttgaa attgatattc
     8761 cagaaaatcg actaatggta aatctcgacc cagatggata ttcccgtgtg gttaataatc
     8821 tggttcaaaa tgttcttgct cacagtaagg ctcggcaaat caagattacc atgagtgagg
     8881 attccagaat ggtattactc cgtgttgatg atgatggtgt tgggattgcc agagaaaatt
     8941 taccacatat ttttgagcga ctgtataagt gtgataaagg acgttcagaa aaaggaagtg
     9001 gtcttggtct atctattgtc agtcaaatgg tggagcgaat gggtggtcag atttccgtag
     9061 aaagtgagat tggaaagtat accgtattta cagtgagctt accattaata taaaattgtt
     9121 tttcccccat gtgacatgta tacacagggg gattttttgt aagttataaa gttgcaaggt
     9181 taatacaagg ttgttgcaag gttaatgtaa gattgaggtg gtatgatagc tttaataaac
     9241 aaaggaggat tttattattt atgaataatt taattattga aacaaaaaat ctcactaagc
     9301 aatatggtgc tcaaaagagc gtttctaatt tgaatattca cgttcagaaa ggtcgcattt
     9361 acggattgct aggaagaaac ggtgcgggta agacgacaac tatgaaaatg ttattgggtc
     9421 taacaaaacc aactactgga gaaattaaaa tatttggtca ggacattcac aataacgaga
     9481 agaagcttct tcctcgaatt ggatgtttga ttgaatcacc agggttttat cctaatctga
     9541 caggaacaga aaatctcaaa atctttgcaa ggcttagagg aataccaaaa aaaaatgcag
     9601 tagaatcagc actggatgta gtcggtttgc caaacaatga caaaaagtta ttttctcaat
     9661 actctcttgg tatgaaacag aggttggcga ttgctcttgc cattatgcat gacccacaac
     9721 tgttgatttt ggacgagcct atcaatggac ttgatcctat tggtattgca gaagtaagaa
     9781 agtttattcg tgatctctgc acagaacgag gtaaaactat tttgatttcc agtcacattc
     9841 tttctgaaat tgccttgttg gcagatgaca ttggtattat tgaccatggt attttgttgg
     9901 aggaagaaag tcttgcccaa ttagaagaaa aaaacagtaa atacatacat tttgctgtat
     9961 ctgatgcagc acatgcagca aggcttctgg aaacccaact taacctgaaa aatttcaaag
    10021 tagcagataa ttacaatatt atgctatatg atacagatgt ttctgttgca gaaataaatc
    10081 gtgctttcat tctaaacaac attgaggtat cagaatctca tttatgcgaa gatactcttg
    10141 aagattactt caagaaagta acagggggtg aaggaattgc ttgatataat ttcatgtgaa
    10201 ttttcaaaac tcaagcggtt aaaatttata ctgatttcta ttttaggtgc ttgcttattt
    10261 ccaattcccg caacaatttt gattgcaaag gacaacttgc cctttgagca attgttcaaa
    10321 ttagttgtca attttggata ttttttgcta ttaccaattg tattaagtat tgttgctagc
    10381 caactatttt ttatagaaag agataacgat gtattaaaaa atcttgcaac agtgccagtg
    10441 ccaaaaggaa aactggctct ggcaaaattg gctgtgttgc tgtttatatc attgttctac
    10501 tccgttgcag ggttaggagc tactatcatt ggaggtttca ttgttggaat agtagagggg
    10561 gttactgtaa aattaggaat gagcattgct tttggaatta tgttttttgt tactgtactt
    10621 cctgttgtgg tactcattgt atatttcaat cgcagctata ttttttctat tatactttct
    10681 tttgtatatg ctattttcaa ttttagtata tccttaaata ttatcaattt tgaaccaaat
    10741 aacccactat taagcgtatt acctgctcct gttataatgc gttggtggat ggcattttgg
    10801 ggagatccca ctggcgaata cacggcactt agacagccat atttgctctc aactcctgct
    10861 tgtgtaggta ttttatgttt gataaccatt atagcagtcc tgctgatttg tatgatatac
    10921 aaaaagcagg agaattagga ggtatctatg cttgatataa ttactacaga atttcaaaaa
    10981 ataaaaagat ataatattct atggattggt gttgttgctg ttctgttttc agcactattg
    11041 gcagtttttc aggttgtaag ttcacacggc agtgaccctc taacgtatga gagctttgca
    11101 aacggagtta tctggaataa tttcagtctt agttttccat ttgggattac acttattgga
    11161 ggatatctta ttaaccgtga atatacagac caaaccttaa aaaacatcct aactgttcct
    11221 atttcattac gcaaattgct agtgggaaaa ttaattgcgg ttggaggaat tgctgtattg
    11281 ttaagtcttt tcagcttctt atgcacattt attttggggt tgttattttg caatatcaat
    11341 atatctgtta cactcattgt aaaatcctta gttcagattt tgtctgttaa tctgtattgt
    11401 tatgttgctg ttttgccgat tattgcttat tttggtcgta aacaaaatgc atttctgacg
    11461 ggtgtaggga ttgcatttgt atacgggttt tgtggtgtgt ttgttgctgg aagaaatctg
    11521 acagactttt atccaatcac agcaggtctt ggtctagttg gatacaacaa tggtgctggt
    11581 actgtttatc agccacttat cggtgctatg actttgattg taattctaat cttgacaact
    11641 attctacttg tatttacacc taattatgat aaagttatga ccatatctaa gaaaaaaggg
    11701 ataaagcata aaaagtattc tcataaataa cataaatatt gtttctatta tttaagttgc
    11761 tagacagtga aaagaaaaaa tctgtcgtac agtggctata tgctcatgta ttcaagaagc
    11821 tacagttttg atttttcata actgtagctt ctttttgtat ttattaaatc acggctatca
    11881 taatacttgc aataggtata gcgacaagag agagtactgt catgtgtgtt ttatatatta
    11941 cattaagtat attctaaaat atatgtttta aataatatat ttgaaaatag gaatatttaa
    12001 ataaaaaaaa taatattata tatagttgac aaaaattggc aaatgatata atatataaat
    12061 atagaaatag taatcattac tattattaaa aagggaggaa ttaattatga acttaaaagg
    12121 aactaaaaca gaaaagaatt taatggcagg atttgcagga gagtcagaag caagaaataa
    12181 atatacatac tacgcttcaa aagcaagaaa agaaggttat aatcaaatag ctgctatatt
    12241 tgaagaaact gctaacaatg aaaaggaaca tgctaaatta tggtttaaac ttttacatga
    12301 tggtatgcct tcaacagagg aaaatttaaa ggatgcagca gcaggtgaaa actatgaatg
    12361 gactgatatg tatgcaaaat ttgctaaaga agctagagaa gaaggatttg acaagatagc
    12421 ttatttattt gaagctgttg gaaaaataga gaaagagcat gaagaaagat acttaaagct
    12481 tttagaaaac ttaaatgaag gaaaaatatt taaaagagat gaagaagttg tttggcaatg
    12541 tcaaaactgt ggtcatgtgt atgtaggtac tgaagcacca gaaaaatgcc cagtatgtga
    12601 ccatccaaaa gcgtatttca atataaaagc tgaaaattat taaaaattat cattattaaa
    12661 aaggaatgtt aactatgaaa ttttctaaac aacgagaact gattttaaat gaaatattaa
    12721 ataatccggt tcatcctact gcggattacc tatatgaaaa cttaaaaaaa gataatccaa
    12781 atttaagctt aggaactgtt tatagaaatt tagctcagct aacggaacat ggctttataa
    12841 gaaaggttag tattccaggg tatccagata gatttgatgg tcgaatagat aatcactatc
    12901 atatcatatg tgaagtatgt ggagaggtat acgatttaga atccgaagtt cttaataatt
    12961 tacaagaatt aatatctgag gagacagaca taaaaataac atcttataac ataagtttta
    13021 aagggatttg taataattgt aaaaggtgta gccaagtagg ttagttttca aattctaaaa
    13081 ataattaatg aggtgagttt atgtgtagtg aacaaaaatt ttttatatgt gagacttgtg
    13141 gaaaccttgt aggaatgata caaagtggtg gtgtacctat tttctgttgt ggaaaaccta
    13201 tgaaggaact tgttcctaat acaacagatg cagcagttga aaaacatgta cctgtaattg
    13261 aagtggatgg aaataatgtt actgttaaag taagtagcac aactcatcca atgactaaag
    13321 aacatcatat agcatgggtg tatcttatga ctgaacaagg tagtcaacgc aaatgtctag
    13381 cagtagatgg agaacctgta gttaaatttg ctttgaatga tgatgataaa gtgatttctg
    13441 cctatgctta ctgtaattta catggattat ggaaggctga actgtaatat ttaaataaat
    13501 tgagtcaaag aggaggctat aaatgtattt tcatacaaat agcctcctat aaatttatat
    13561 aaattcaagt tttaattttg tttaatgtca atataatata aggagaaaat aatatgtcaa
    13621 tttataaatg tagtgtttgt ggatatattt atgatgaatc taagaatgat aaaacatggg
    13681 atgaattaag tgaagattgg gaatgccctg tatgtacaaa aggtcgtagt tattttggaa
    13741 agataagcac tgtttactat gaagaagatg aaaagatagc agaagacata gttgaaaatg
    13801 aatctaaatt aaatactgaa aaagagggag atttaaatta cttaagtaca tatttaagaa
    13861 gagatgatga agttgagaaa catatggata taattcacga aatggctgtt acaggaaaat
    13921 ctataataga acctatgaga actaaactac cagtaatttc ttgggatgat attttaatta
    13981 tgggtgctca attaaatcca ttacctttaa atgagcatga tgaagtaaat acaacaacaa
    14041 taataggaaa aaaagcaaag aaacctatga ttatagaaaa tccagtttat atttcccaca
    14101 tgtcatttgg agctttgtct aaagaactaa aaatagcttt agcaaagggt gcagcccaaa
    14161 ataagacagc tatgtgtagt ggtgaaggag gtatattacc agaagagaag gaagcttcat
    14221 acaagtatat ttttgagtat gtaccaaata aatatagtgt aactgaagaa aatcttaaaa
    14281 attctgatgc tatagagata aaaattggtc aaggaacaaa acctggtatg ggaggtcatc
    14341 ttcctggcga aaaagtaact gaagaaatag caaaagtaag aaataagcct gtaggtcaag
    14401 atgtaataag tccatcttgt tttgaggaaa tacaatccaa agaggattta aagaaactta
    14461 tagatgaact tagagaagtt tcagaagggc gtccaatagg tgttaaaatt tcagcaggtc
    14521 atattgaaaa agatatggaa tttatagctt atgctaaacc tgattttgta actattgatg
    14581 gtcgtggtgg agctactgga gcaagtccaa agttgcttaa agatgcaact tcaattccaa
    14641 caatatttgc tctttatcgt gctagaaaat acatagatac acatggatta gatatagatt
    14701 tggttataac aggtgggctt cgtatatcta ctgattttgc taaggcaatt gctatggggg
    14761 ctgatgcagt tgctattgca agttctgctc ttatggcagc ggcgtgtcaa caatatcgta
    14821 tatgtggttc tggtaagtgt cctgtaggag tagctactca ggatgaggaa ttgagaaaaa
    14881 gattacatat agaaaatagt gctaatagag tggcaaactt tttaaatgta agtttagaag
    14941 aacttaagac ttttgctaga atttctggtc ataaagatat acatgattta agtgttgatg
    15001 atttatatac tgttaactct gaaatatcaa attatacaaa catacagcat gtatagattg
    15061 aagtttgaaa ctattagatt ttagatagct ataaaagtaa atgataaata tattttataa
    15121 aagagagtgt cttaaaatga actttttagt tcataagtca ctcttttttg tatgaagcca
    15181 aatatattta tcattttagc aatgaaaaag aatcttatct ctttattttg agatagactc
    15241 cttctattta tgaaaattaa agatattaaa ttgtaaaata ttctgtgatg tcgtactatt
    15301 aaactaaaga ttctgtactt taaagtaaat aaaaatttaa ggagtgctta ttatggaaag
    15361 attgaagata caagttttag ttgacaataa tacttatatt gatagatact ttgttggaga
    15421 accagcagta agctattata ttgagattga tggaaatagg attttgtttg attcgggata
    15481 ttcagatgta ttcatatcaa acgctgaaaa attaaatata gatttaggaa atttaactca
    15541 tgttgttttt tctcatggac ataatgacca tacaagaggt atccagtttt tagaaaatag
    15601 gtatgattta tctacagttg aacttatttc acatcctaac tgttttatac ctagaaaaca
    15661 tggagagaaa agcataggag ctccattttc tgctgaggaa attaaaaata tttttatgta
    15721 taatcctaag gataagccat ttaatctaag taagaattgt gtgtttttag gtgaaatacc
    15781 ttctataaat aattttgaaa aaagagctaa aattggtaaa tgtaaaatag gagatttgtg
    15841 ggaagatgat tatgttttag atgattctgc tatagtttgt aaaactgata aaggtatttt
    15901 tattgttaca ggatgttctc atagtggaat ttgtaatata gtagagtatg ctaaaagggt
    15961 atgtggagat gatagagtta ttggtatact aggtggtttt catctttttg aattgaataa
    16021 taggttagat agtacaatac aatatttgga taaagaagaa ataagaatgt tgtacccttg
    16081 tcactgtgtt tcttttaagg taaaagctaa gatgaatgaa atattgaata taaatgaagt
    16141 tggagtaggg ctgaccataa gtatatagta tttgaaagta tataagattt aagttgaagg
    16201 ttaaaaagat attttctata taaagtatct tttttgttac gtttatttta agagaaatca
    16261 atatactttt atatgaaata ttgtaaaaaa atttattttt aatgagaata tattttcatt
    16321 aataaacaat tttattatgt attaataatg ctagaataaa tataaataaa tcaaagttac
    16381 ttacattaaa gtatattttt tcacaaaata tattgattta ttcttttaaa gtttgataat
    16441 tccaatgaat gtaataaaag aagggatttt atactataca attattatta taaaattata
    16501 aagaaaaaat taaataataa taaagaaaaa atgtataata tttaacatta aaaaagatat
    16561 ttcataaaat taaaaaacaa agtgaatttg aatactattt aaaaaataaa aaaatatata
    16621 aaaaatattt aatacaaagg tagaaataag ttgaaaatgt aaagtcattg gaaaaatata
    16681 gttgaattta atgaacaaaa atgttattgt tttatcaatg aagtgttaaa ataaaaactt
    16741 tcaatatcat tttagggagg atgatttcat gcaaaaaagt gtaaagaaag caaaagtaac
    16801 gggatctatg ttagccattt ttggtgttgc aagcgttctt tttagttcac atgcaggagg
    16861 aggatttgct acagggaatc aagaaactca gtactatgtg cagtatggat ggacagcacc
    16921 acttatggca attttagcaa tgattatact tactgctacc atgagagaag ttatcataat
    16981 gtataataat aacaattgta ggaattataa ggatttattt tgtgagcttt ggagaccata
    17041 tccaaaactt gaaataatat gggagatata ttactatcta atggttttaa tagctgtaag
    17101 tgctgttata gcaggtgcag cagcagtatt tcaaagtata ggagtaaatt attttgtagc
    17161 tgtatttatt attggagttg tacttcttgt ctttactata tttggtgcta tgcttgtatc
    17221 taaagcagct acagcaatga caatagctat tctagtatgt actcttacta tttttattgt
    17281 tggtataaaa gctaaagtac cagaaataac tgaaatatta tcaaatagaa cgagtttcac
    17341 acctggctat atcaagccaa tacttaatac ttttatttat gcaggttttc aatcggttgt
    17401 tatacctaca cttgcaggat gttcaagacc acttcttaag aactcaaaag aagctactaa
    17461 ggcaatgata atttcatttg ttatgaatgc aattgcatta ggacttgctg taactatgct
    17521 aatgggatgg tatagagaaa ttattgctgc tgggcaaaca acactcccta ctctttatgt
    17581 tgcaggtcaa tctggaaatc atactatata cataatttat aatgttgcat tattcctttg
    17641 tttaatgtct acaggagtaa ctactatatt tggtcttgta aatagatttg aagaccataa
    17701 agcactttct ttcctttctt caagaatgaa aagaagggta tttacagctt gtgcaataat
    17761 ggtagtgtca atgcttatat cacttacagg attaagtaat attgttaaat atggatatgg
    17821 atattgtggt tatcttggtc tattcactat agtaattcca ttccttacac taggacatta
    17881 taagaataag aagtttgcaa aggaaaatcc agaagctaaa tggccagcgg aattaaatga
    17941 aaatgtaaat tagatgtagt agcaaaaaat attgctaaag agaatactat aaataagaat
    18001 actatagata atatatctgc atgtttatta agattatttg caaaatctaa tattgaacaa
    18061 ctataaacaa gcaatataaa actaagaata aaagataaaa agagggtgtc tcaaaatgga
    18121 cttttagttt ataaggcact cttttttata tgaagtcaaa tatattttca ttattttgac
    18181 aataaaagag actaatccta ctttggatag cctctttttt ctttaatata aaatagtata
    18241 acagcaaagt atagattgat acatgttttc tgtagctttc tatatattac agtgtttgtg
    18301 tggcattggt attttaagca atctataaat tataattcta taattcttct actaatttta
    18361 gattctatag ccgtctctat ttcacttaac attcgttcat ttttagggtc aataaaagca
    18421 catgtatagc cttcttcacc agctctacca gttctaccaa tacggtggat gtagtcttct
    18481 ggtgtttctg gtaaatcgta attatatata tgacttactc cagatatatc aaggccacga
    18541 gaagctacat ctgtagctat taagtattgt atatctaaat ttctaaatga tttcattatt
    18601 ctttctcgtt ttgattgagc aatatcacta tgaagttttt gacaattata tcctcttata
    18661 gcaagagctt cttcaagatt gtcaaccctt ctcttagtcc tacaaaatat tattgccata
    18721 aaaggattat cttcatctag taccttacat aaggcatcta atttttttct atctgtagtt
    18781 tctactactt cttgttttat attattaaga gtgatttctt ctttttgaat agaaactaca
    18841 acaggatttt ttgtgtatct ataagctaat ttttttactt gagaatttat agttgcagaa
    18901 aagcatagtg tttgcttttt gtttgaagtc tcttttaaaa tagcttccac ttcgttttta
    18961 aatcccataa gcaacatttg gtctgcttca tctaaaacaa aagtctttaa cttactaagg
    19021 tctattgttt ttctatttag atggtctaaa agcctacctg gtgtagctat aatcatatga
    19081 atatttcctt ttagtttctt tatttgagaa ccaatatctt ttcctccata tgctgcaaga
    19141 atacttacat ctttagattc tttaagtttc atagcttctt ctgttatttg aatagctagt
    19201 tctcttgtag gagaaagtat aagtacttgt atatcattta tatctaatga tatattttca
    19261 aatatcggaa gtaaaaaagc tagtgttttt cctgttccag tttgagcttc tgctattaca
    19321 tcttttcctt cttttattag tttaatactt tctttttgta taggtgtagg tgttgttatt
    19381 cctgattttt ttagaatatt tattgtattt tcactaatcc ctaagctttt gaaatccatt
    19441 gttatcttac ctttcatgaa aaattttctt ttttattaac caattaataa tatcatattt
    19501 gtaagtattt ttagtaaatt ttttaattat tatttatata gcttaaaaat atattaagat
    19561 actttaaaat ggaatgtaac ttaacttaaa ttttagtagg aattttatag gtttattttt
    19621 aaaggttttt tgaaaataaa tctatttaaa aattaaatta aaatgcaaga aaaacataat
    19681 aactttcatt tttaagaagt tttcacaaaa aagaaaaaat atagtgcaat aaatgataca
    19741 aagtaatata atagattaca agatatgaaa agctaaaaaa ataaattgca aatgaaatta
    19801 aaatgaataa aaaataaaaa aataatcata agttagaggt atcaatgaaa aataagactt
    19861 aatgttttta aaattaaaag tatactaagg ggggcattaa tatgtgcgtt atatcaaaag
    19921 atagagcaaa ggaaataaaa atagtagata ctactttaag agatggagag caaacagctg
    19981 gggttgtatt tgcaaataga gaaaaaataa tgattgctga aatgttaagt gacctaggtg
    20041 ttgaccaaat tgaagttggt atacctacta tgggtggaga tgaaaaaaat gtaataaagc
    20101 acatatgtag tagaaattta aaatcagata taatggcttg gaatagagca gtaataaaag
    20161 atgttgaaga atctatttct tgtggtgttg atgcagtagc tatatcaata tcagtatctg
    20221 acattcatat agaaaataaa ttgagaactt ctagaggctg ggttttagaa aatatggcta
    20281 aaacagttga atttgcaaag aaaaatggat tatatgtttc agtaaatgga gaagatgctt
    20341 caagagcaga tatagacttc ttaactgaat ttataaatgt tggaaaacaa gcaggggcag
    20401 atagatttag atactgtgat acagtaggag ttatgaatcc tttttctata aagaatgcaa
    20461 tagaaactct ttatgaaaga actaattttg atatagaaat gcatacacat aatgactttg
    20521 ggatggcaac tgcaaatgca cttgcaggaa tagcagcagg agcaaattat gttggtgtta
    20581 cagtaaatgg attaggtgaa agagcaggaa atgcagcatt agaagaagtt ttaatggcac
    20641 ttaagtgtgt ttataaatgt gatttaaata atatagatac tagaaaattt agaggaatat
    20701 gtgagtatgt agcacaggca tcaggaagaa tacttcctac atggaaacca gtagttgggg
    20761 ataatatgtt tatacatgaa tctggtatac atgcagatgg agctttaaaa gacccgcata
    20821 actatgaacc atttgaccca agtgaagtaa atcttgaaag aaaaatagta ataggtaaac
    20881 attcaggaag agctgcagtt ataaataagt tgagtgaata tgaaatgtat atttctccag
    20941 aaaacgctac caagctatta aatgctataa gagctacatc tataagatta aaaagaagtt
    21001 taatggataa agaaattctt caattatatt gtgatatact agcacatgag aaaggcacaa
    21061 cagaagaaga agcagttaga ggttcatata tttaagatat aattgtttag gaggaaaata
    21121 tgggagataa tatagtttat aaaataatta aaaaacatat agtagatgga gaagctgttg
    21181 ctggaagttc aattggtata aaaatagacc aaactttaac acaagattct actggaacaa
    21241 tgacatatct tcaactagaa gctatgggaa tagataaagt aaagactaaa agaagtgtgg
    21301 catttgtaga ccataatatg ttacaacaag gatttgaaaa tgcagacgac cataagtata
    21361 tacaaacagt ggcagataaa tatggagttt atttctctaa gccaggaaat ggtatttgtc
    21421 atcaagtatt tttagaaaga ttttcaacac caggagatac acttttaggt tctgatagcc
    21481 acacacctac tgctggaggt gtgggcatga tggctattgg agctggtgga ttagatgttg
    21541 ctttagccat ggcaggagga gcatactata taaaagcacc aaaagtctgt aaggtgaatc
    21601 tagttggaaa attaaataat atggtatcat caaaagatat aatattagaa gtgttaagaa
    21661 aacaaactgt aaaaggtgga gtaggtaaag tttatgaata tggtggagaa ggtgtcaaaa
    21721 gcctatctgt tccacaaaga gctacaataa caaatatggg ggctgaactt ggagcaacta
    21781 cttcaatctt cccaagtgat gaaaaaacac tagaattttt taagagtcaa ggaagagaag
    21841 acgcatggat agagttaaaa ccagatgcag atgcagttta tgatgaagag attactataa
    21901 acttggatga attaaaacca ttagctgcaa aacctcatag cccagacaat gtagatgaag
    21961 ttgaaaatat aggcaagatt aaaatagacc aagtggcaat aggaagttgt acaaattctt
    22021 cttatgaaga cttaatgaaa gtagcacaaa tcctgaaagg aaataaagta cataaggatg
    22081 tgagtttggt aatagcacca ggttcaagac aagttatgga gatgattgct agaaatgggg
    22141 ctttggcaga tataataagt gctggagcta gaatactaga aaattcttgt ggtccatgta
    22201 ttggtatggg tcaatctcca ggtactgact ctgtgtcttt aagaactttc aatagaaatt
    22261 tctatggtag aagtgggact ttgtcagcac aagtatattt agtaagtcca gaagtggcag
    22321 cagtaagtgc aatcaaggga gtgcttacag acccaagaga atttgatatt aaatttacaa
    22381 atttagatgt aaatgaattt ttgatagatg atagcatgat aattaaacct gctgatgtag
    22441 gaagtaatgt agaagtagtt agaggaccta atataaaacc tttccctctt aatacagagc
    22501 taagtcagtc tataggtgga aaagttatat taaagacaga agataatata actactgacc
    22561 atataatgcc atcaaatgct aagttacttc cttttagaag taatataccg tatcttgcaa
    22621 actattgttt caatactgta gatacagaat ttcctcagag agcaaaagac aataatggtg
    22681 gatttatagt tggtggagat aattatggtc aaggttcaag tagagaacat gcagctttag
    22741 caccattata tctaggtgtt aaaggggtaa tagttaagag ctttgcaaga atccacaagg
    22801 ctaacttgat aaatagtggt ataattccaa tggagttttg tgatgagaaa gattatgaaa
    22861 atatatctct tttagataat ctggagatac caaatatttt agacaatcta ggtagtggaa
    22921 ttttagaagt taagaataca actaaaggaa cttcttttaa ggtcaaagta gaattgtcag
    22981 caaaagaagt tgacgtatta aaagctggtg gaaaattaaa ctacactaaa aatcaagcta
    23041 attaatatga aaagagggaa tattgaatgt ataaagtgac acttatacca ggagatggaa
    23101 taggaccaga agttgcgaaa gctatgaaaa aggtagtaga agctactgga gcagaaatag
    23161 aatgggaaga agttaatgca ggagaggcag ttattgaaga gtatggtact ccacttcctg
    23221 aatatattat agatagtata aaaaagaaca aaatagctat aaaaggccca attactactc
    23281 ctgtaggtaa aggatttagg agtgtaaatg taactttaag acaggcatta gatttgtatg
    23341 tcaatttaag acctattaaa agttttaagg ggataaaatc aagatatgaa gatgttgatt
    23401 tagtggttgt aagagaaaat acagaagatt tatatgctgg aatagaacat aaaataggtg
    23461 attatgcagc tgaaagtata aagataataa caagaagtgc aagtgaaaga atcgttgatt
    23521 ttgcatgtaa ctatgttaaa gataataaga gaaaaaaagt tacagctata cataaggcaa
    23581 atataatgaa aatgtctgat ggattgtttt tagatgtgtt tagagaagtt gcatcaaaac
    23641 atggtgtaga atatgatgat ttgattgtag atgcagcagc tatgaactta gttttaaatc
    23701 cagaaaatta tgatgtaatg gtaatgccaa atctttatgg agatatacta tcagatttag
    23761 gtgctgggct tgttggaggt ttaggtataa ttccaagcgc aaatattggt aaagattgtg
    23821 cgatatttga agctgttcat ggaagtgctc cacaaatagc aggtcaaaat aaggctaatc
    23881 caacagcact tattcaatca tcagtaatga tgcttagata cttaggtgaa tatgaaaatg
    23941 cacaaaaaat agaaacagct ttagaaaaag tatttcttga aggaagtaaa ttgactgtag
    24001 atttaggtgg ctcagcttct actacagagt ttgcggatga ggtgtgtaag tatattgtat
    24061 agattttatt tagggaaaaa gtgaaatata gattgaagga aagaggcttt ctcaaaatag
    24121 agataagcct ttttttattg gaatgataaa atatatttga ttttatacaa aagagagttc
    24181 ctcataaacg aaaaagttaa ttttgggata tccattttaa ttttaaaatt aattgattaa
    24241 tacgcaaaga aagattaaac aaatttatta tttttaattg aactttcaac caaaatatgg
    24301 taaaatttaa aaaagttgaa ctttcaacta aaaataaata tattgattat gtgaaattct
    24361 ataaaacaga aactatttga ctttagtcat ggtaggttca gtggaggtgt gtttgattaa
    24421 gactttagat agtaatatat taagagaagt aggtacttta tctagagcag taaattcaat
    24481 aaatgatatt aagtacaagg aattaaagtt acaaaaagga caatttacat ttttgacaag
    24541 aatctgtgaa aatcctggta taaatcttgt ggaattatca aatatgttaa aggtagacaa
    24601 ggctacaaca accaaggcta tacaaaaact aataaaggca ggatatgtag ataaaaagca
    24661 ggatgagttt gataaaagag gatataattt aactccaact gataaatctt tggaagttta
    24721 tgagttgata attgaagaag agaatagaag tatagagata tgttttgata attttacaga
    24781 tgaagaaaaa caagtggtaa ctaagttatt agaaaaaatg agcaaaaacg tagaaaatga
    24841 atggtttaaa gtaaagagat agtaaataaa tacattaact taaggagata tttttatgat
    24901 tagaaaagcg aatatgaatg atttagaaag cattatgaaa attataaaat ctacagtgga
    24961 agaaatgaag acttataata atacacaatg ggatgaaaac tatccattag aaaaagactt
    25021 tgtttcagat attaagaaac aagatttata tatttatgaa gtggatggag aagtagcagg
    25081 atttatatgt cttaattatg aagagccaga agaatattta ggcttaaatt ggtcatctaa
    25141 taaaaaagca atggtaatac atagaatggc tgtaaatcca aactttagaa agacaggaat
    25201 agcatctaag ttagttgatt ttgctgaaaa attagctgta gaaaacaatg tatcatactt
    25261 aaagagcgat acatattcaa taaactcaaa aatgaattta ttacttacaa agtgtggttt
    25321 tataaaaatc ggagaaatga gttttttagg aaaggagaaa tctttctact gttatgataa
    25381 aatattataa agagcattta taatacaagg ggtaaataat agattttcag cattagtaag
    25441 ggcataatat aacccagtat atgctattat gaaagggatg atttatatga ttggatttct
    25501 aaagaaaaga agaagtatta gaaaatacaa ggatgttgaa gtagaaaaag aaaaattaga
    25561 taaaatatta aaagctgctc ttttagctcc atcatcaaag gggcttagaa catgggaatt
    25621 tatagtggta gatgataaag aaaagctaat taatttatct caatgtagaa ctaagggtgg
    25681 tggatttttc ttaaaaaatg cacctttagc tattgtaatt attgcagata aagaaaaaaa
    25741 tgatgtttgg atagaggatg caagtatagc tgcttcatac atacaacttc aagctcatga
    25801 actaggttta ggttcttgtt ggatacaagt aagaaataga atgtatgatg ataatataga
    25861 agctgataag tacattagag aagaattaaa agtacctagc aagtacagtg tagaatgtat
    25921 aatttctata ggatattctg atgaagaaaa gaaagcatac aatgactctg atttagacta
    25981 taaaaaggtt cattttaaca acttttagta tataatatgt tgtattaaga cattatattt
    26041 ttttctattg ttaatatata cttaaaattg taaaaatata tctgaatttt actaacttat
    26101 ctaggggtta tgtacaagga ttttaaagat aaatatagaa attaatgttt ataatcgata
    26161 cataacttga gtaacaaaat ttaaggaggt taaagtatgg gtatatttaa aacaaaaatg
    26221 gatgaggact ggaaagtaaa ctacattaaa gagtttaatg aaatgagaga ttcttatgag
    26281 agtaaacttc aaaaaaaaca gtttgaggta gatagtttga aatcagaatt ggatagacta
    26341 agaagctata aaaatagcct taagccaaaa gaaaaacaaa ttactgatga tgatataaat
    26401 aatataaaga gtttaagaag agatggctta agctataaag aaatttcaaa tcaaacaagt
    26461 tggagcaaag caactgtaag tagggtatta aatggacttt acgattagca tatacatggg
    26521 gtgatttaaa ttgggatgaa gtatagttac aaaaaatgca taatagattc tattttattg
    26581 atgtttataa ttcaaatatt aaggatgatt ttgaattatg tacttctaag tcaatttgaa
    26641 tttactcttg aaaattttaa tattataaac ttaatttctt ttacattagt tggtttatcg
    26701 ttaattttat ttttaaaaga taattctttg tataacaagg ttagaaatag aaaaatcaca
    26761 gaagcttttg aagaaaataa aaacaatata cttattgaaa agtgtaagct tatacttttt
    26821 gttgtggttt tatctttagc aataattgta acttattgta ctaagggata tgtacttttt
    26881 aatgtaacta tgatgacttt gtctgtattg atagttccaa tatttgaaga gttatttttt
    26941 agagaatata tatggaacta tctaagtaat tttataaagt caaagagtaa aattatctgt
    27001 ataacatcta tactatctgg aatttataat ataggataca tagatgttat aagaaattat
    27061 gtaatattgt ataacaattc aagctacacc tttgaagtta ttatttcaaa aattatgata
    27121 ggtactgttt ttggtattgt acttggatta gttaagtata ggtttagaga tgtagggttt
    27181 tgtatattat tgaggagctt atttgctata tttattaggt agatagtata atcggtttat
    27241 aaggttgttt taaatttaat tttaaatcaa ccttatttta tttgtgtttt ataaaacact
    27301 tatatattaa taactaataa atttaataaa gattatttga atatttttaa aatatataaa
    27361 tatgtattga catataaata ttatgaatta caatttatat aaactaataa aaattaaata
    27421 atattaatca acttattgaa tggaggaata aaaatgaaat ttgtgacttt ttgtagtagc
    27481 aatgaagaaa aaataggtgt atttaattca gaaacaaata gtatatatga aattaatagt
    27541 ttaggattaa gtaaattata tactgatatg aatgatttta ttgaaaacgt atctacagga
    27601 gatttagaaa aaataaaaaa caattcattt gaaaacgcaa agtgttataa gttagaagaa
    27661 gttaaattat gttccccaat tgtaagacct aaaaaagata ttatatgttt aggtctaaac
    27721 tataaagacc atgtcaatga aatcccagat ggagttataa aaaacgtagt tatgccagat
    27781 tatccaattt atttttctaa gagagcagac aaaataattg gagtggatga taagataagc
    27841 ttacatggag atttggtaga gaagttggac tatgaatcag aacttgcagt aatcataggt
    27901 aaagaaggta taaatatatc aaaagaagat gcttatgaat atatatttgg atatacaata
    27961 gtaaatgata taagtgaaag agcacttcaa gataaacatg ttcaatggtt tagaggaaaa
    28021 agcttagaca cacatacatc tatgggacct tgtatagtac ataaagaaga atttgaacat
    28081 cctttaaaat tagatataag tagtgtagta aatggagaag taagacaaaa ttctaataca
    28141 gagtacttta tatttgatat acctactata ataaatgatt tatcaagagg aatgactcta
    28201 aagccaggag atataatttc aactggaact ccagcagggg ttgctatggg gatgaatcct
    28261 caagtatatt taaagcatgg cgatatagtt gaatgtaaag tcgaaggaat aggagtttta
    28321 aaaaatatag ttgattaatg gataaatatg ccatataatt ttattagtac aaataattta
    28381 attaatatag gattgatcaa acttatgatt aatttattat agagagggat gagtatatgg
    28441 caagagaact agaattaaag tatggctgta atccaaatca aaaaccatca aagatttata
    28501 tgaagaatgg agaacttcct atagaagtat taaatggaaa acctggatat ataaattttt
    28561 tagatgcatt taatagttgg caacttgtga aggaattaaa agaagctaca ggtcttccag
    28621 cagcaacttc atttaaacat gtaagtccag caggagcagc agtaggggta cctttatcag
    28681 atactttaaa gcaaatatat tttgttgatg atttagagct ttctccatta gcttgtgctt
    28741 atgcaatggc aagaggagca gatagaatgt cttcttatgg tgattttata gcactttcag
    28801 atgtatgtga taaggagact gcaactatta tagcaagaga ggtttctgat ggtataatag
    28861 ctccaggata tacagatgaa gcccttgaaa tattaaaagg aaaacgtaaa ggcaactata
    28921 atattgtaaa aatagatgaa aattatactc ctgagcctat tgaaacaaag gatgtttatg
    28981 gaattacttt tgaacaaggt agaaatgaaa tattaataaa tgaagaacta ttaaaagata
    29041 ttccaactgc gaataaaatt tttacagata gtgcaaaacg tgatttaatt atagctctta
    29101 ttacattaaa atatacacaa tcaaattctg tttgttatgc taaagatgga caagtaatag
    29161 gggtaggtgc aggtcaacaa tcacgtattc attgtactag acttgcaggt aacaaagctg
    29221 atacttggta tcttcgtcaa catcctaaag tattaaactt aaaatttaaa aaggatatag
    29281 gtcgtccaga tagagataac actattgatg tatatctttc tgatgattat atggatgtgc
    29341 ttgctgatgg catatggcaa aacttttttg aagaaaaacc agagcctcta actggagaag
    29401 gaaaaagagc atggcttaaa actttaacag gagtagcact tggttcagat gcatttttcc
    29461 catttggaga taatatagaa cgtgcaaaaa gaagtggggt aagttttata gcacaaccag
    29521 gtggttctat tcgtgatgat aatgttatat taacttgtaa taagtataac atagtcatgg
    29581 catttactaa aaatagatta ttccatcatt aaaatactga aagtataaag ataatataga
    29641 ttaagtaaaa atctacaaca agttttgttg tagatttttt tatttgctgt ataattttaa
    29701 atagaacttt atttttagat attcaatttg gaggttgata tggctttaaa tataagaaac
    29761 tatagcctat tatgtaatca aggaacagct aaatttaatg aagatgtagt cggtataaat
    29821 ccatttgggg catgggtttt agatggtgct acaggactta atggaaaaaa tttagtgtca
    29881 aatgaaagtg acgcaaggtg gtatgttcag tggtggaata ggtatttata taaaaatata
    29941 aataaagaag aatcattaaa gagtattatt aatactggaa taatagaagt aaaaaaagag
    30001 tatgaactaa gattaaatgg gtttaaggtt gaaaaattag atttaccatc tagttctata
    30061 gttgttgtaa aatttcatga aaataaagta gagtattttt tattaggaga ttgtgctttg
    30121 tttattaaaa atggagattc caaagttata aaagatagga gtatttgtaa atttgataat
    30181 actgtatttg atgaaatgag taaactttct aacttggaag agatggcatt tgatgagatt
    30241 agaactagtg ttatggatac aatagtaagc aatagattga agaaaaatac gaaagagggc
    30301 tattggatat tggattttga tgaggaagct gtggaaaatt ctatatatgg atacattgaa
    30361 ataaaaaaag attttcaatt gatgctaacg agtgatggtt ttacaagtgc atgtgataga
    30421 tataatctaa taaaagaaga agaattgata cgtatagcag aaaaatttgg tataggatat
    30481 atacataata aagtaaggga ttttgaagat aatgactata aagcggttaa agtgccaaga
    30541 tttaaaataa aagatgattc atcttgttta tatttagata tatataatga ttagactgta
    30601 taaaactatt taattttaga aaggaggcaa gtgaaaagtg aaaattcttc atataataac
    30661 acaaaaacca aatagtacag gtagtggtat atatttgtct ggtatgataa aagggttcga
    30721 aaaaatcggt cataagcaag ctgtaattgc aggtatagat gtaaatgatg atgtaaattg
    30781 ttttccaagt gaagtaagtt tttatccagt aaagtataat tgtggggagt taaattttcc
    30841 tgttgtaggt atgagtgatt ctatgccata tgaaagtaca agatataaag atttaaatat
    30901 agacatgata aatagattga aatatcaatt taaagtaaat atagataagg ctatgaatga
    30961 ttttaaacca gaacttataa tatgtcatca tttgtacctc ttaactgcat ttgttaggga
    31021 aatagtaaaa gatataaagg ttatgtctat ttgtcatgga acttgtttaa gacagttaaa
    31081 taccattgat ttagagaaag aatacataat agcaaatata aggaaattag atttaatatt
    31141 tgctcttcat gaaaatcaga aatatgatat tataaagact tttggagtaa gtgaaagtaa
    31201 agtagttgta attggtagtg gttataatga tgatatattt tataataaaa attataaaat
    31261 aaaggatgat aaaataaaaa tagtatttgc aggtaagata tgtaaatcaa agggattgat
    31321 tccatttata aaatctatat ctaaattaaa atatagtaaa gatttaatag aagtcaattt
    31381 tgcaggtact ggaagcgata tcgaatcata taatgagata gttaaactgg caagtaagtc
    31441 tccatttaaa atgaatttcc taggaaagtt agaacagaga gatttagctg agctattcaa
    31501 caggtcacaa atatttgtgt taccatcatt ttatgaggga ttaccagttg tagttttaga
    31561 agctttatct tgtggcacag atgtaatcac tactgatatt cttggtgtta aagagtggat
    31621 tggtagtgag attaataatt ctggaaagat agaatatatt tctctacctt ttatggagaa
    31681 agaaggtatt ccaaaagatg aagagttata tgattttgaa aataatttgt ataatgcaat
    31741 agattctaag atacaatctt tattgaataa ctcaaatgag aaaacatctg tagatatgtc
    31801 aaaaaaaacg tgggatggct tagcatatag aataaatgaa gttattttaa tggatgaatt
    31861 gtgtttagtc taaaattatt taaattataa aaaagtgtaa ctatttttaa acttttttta
    31921 aatgatttta tattatacta atactaaata tatttgtttt agggaggtaa tttatgagga
    31981 ttagtaaaaa ggtattggcg ttaggtattt cagcattact gttaagtgtt agttttccaa
    32041 catcaataaa cgcactagat aagattgaaa atattcaagg agtagataaa tatgaaacag
    32101 ctgggcttat ttcagataag caagatttta caacagcagt tcttatcaat gcagattcta
    32161 caatggtaga tgggattgct gcaagtggtc ttgcaggagt aaataatgca gctattttat
    32221 taactaataa ggatgatata ccagaagcaa cacttcaaag actgaataga gttactaaga
    32281 tatatgtaat aggtggagaa aattctataa gtaaagatgt agaaaaaatg ttacttatga
    32341 gaagaatgca agttataaga attgatggtg tagatagagt agatacaagt tataaaattg
    32401 caggtgaaat agaaaaaatt aaaaacagtg ataagatgtt tttagtaaat ggatttaagg
    32461 atgaagctga tgcagtaagt gttgcatctg ttgcttatag agatggagct cctataatat
    32521 tgacaaaaga tataccttca gcagaagaag acacagactt agacccatgg tttggagttt
    32581 ctcctgttcc agtatatgct attggaggag agtctacatt aagtgattac atagtgagca
    32641 gatatagagc aactagaatt ggcggtgttg atagatatca aactaataaa aatgttattg
    32701 aaaagttcta taatggagca aaagagttct atataacaag tggagacgat ttagtttatg
    32761 ctttagttgc atcaccactt gcaaaaaatg ctcctgttgt tttagtttca aataaaagtg
    32821 ataagagtat attaagtgga gctagtaaag taacagcaat aggtataagt gataagagta
    32881 ttatagagca atgtttggat gctgtaaaga agtaatggag tgttattagt aaaatgaata
    32941 tatacattga aaattagtag attgttcatt ttataatggt ataaaatata tgggaagagg
    33001 ctatcaaatg gagataagcc tcttttttat tgataaaatt atgaaaatat actttatttt
    33061 atataaaaaa agtatctcat gaactaaatg tctattttga tatactatct ccttttctta
    33121 tattatatgt attatatgat tttttatatg gaataaattt cataagtatt atcttccttt
    33181 aatttttcta accaaatcat ctctaaaata tctagttctt ctaaatagtc tttctctgtt
    33241 ttgttatcat catgttttat aagttctaaa acttcaaatg taaatgcttt ttcaccatat
    33301 tttttccatt cattatttaa ttcttttatc atacatgagc cagcaccaag ctgaaatttg
    33361 agagagttaa atttgctttt tatgtcatta ctttttccaa ggtaaatctt tttagtaata
    33421 tcatttttta ttatgaatac acctgtgtca aatttcattt ctttgtataa ttgttttaat
    33481 tcacgttttc tatccatgat tttagatacc tctctttata attgatatgt aatatgtaag
    33541 tttaaaaata tattacatat caattataaa gagattaatt ttatatgtaa atagttttta
    33601 taaatttaat tatgaattag tcatcaataa acaagaaatt tagctgtaga aatccattat
    33661 aaaggctatt ataaatttta atatgctaaa aatataaaaa gaatagttga cagtatagaa
    33721 aatatggaat aaactaaaag tgtaatagta atacgaaatt atatattaca ttgaaaggtg
    33781 tgaatgatta ttatgaataa aaaaatagaa ttaataggga attttgcaag tgattggatt
    33841 aggtatagtg attatgaata taaaattact gaagaagatg aattatatat agttccaaca
    33901 gaagatgctg tgtttaccat gtataaccca tttgatgtag cagatgatat aatagtagat
    33961 ataattcgca taggacaaga ggcattacaa gatatatcaa aagagacaaa agaaatgaaa
    34021 ctaaagaaag aagtacttga atttgtaaaa aagtatggat tattgggttt aatatattca
    34081 agtgtatata atagagatat agttggagaa gaaagagtac tgatgattga aaagaattat
    34141 attacaaaag agaatatatt gagtacagat gaatatgtaa acatgtttat accatttgca
    34201 agtgatgaag atgtgtattt taaaaaatat agaaggggta tagatgtatc aaagagagaa
    34261 gaatctccaa agttttttgg aaagagacca ttgattttag atttagtatt ttcaaagttc
    34321 tacactgaac aactagattg gattgtaagt tttgctaaag atatgtgtaa gcattttaat
    34381 caaatttgtg tatataaaaa tacatctaat ctattaactg aaagtgttac tattatgtct
    34441 ggagggttta atcctcaaaa gataggattt tctataagcc aattagataa accaattatt
    34501 gcatgggagt ttgattcttt gaaaacagct attgagacta tctatgcctt tgcaattaca
    34561 agtgaacctt cttctttaaa taaatgcaag tactgtggta aaatttatat tcctacaaat
    34621 tctaaatctc aatactgttc accttcttgt agaaattgct caaatgtaaa gaaaagtaga
    34681 agtagagcca aaaaagataa ataaaattaa taaatatatc attgtttaga ttttagtaaa
    34741 taactactga attaaaagtt gttatagaaa attatatatc taaatgatgg tatttttttg
    34801 ttgagaaaat tgaaaagttt aattgagata gtaattttga gttgacaaaa accactttta
    34861 acactactat tataagtggt tggtaaaata atatacacta atttaaatat agcataaaaa
    34921 acaaaaaaag tcaatgaaaa aaataaaaaa ggagaattta aaaatgatat cacctattat
    34981 tttgagttct ataaatcaga atttaaagga aatagaaaga aatgaactgc ttgaaactaa
    35041 tatagaatct ggagattatg gtctagctct ttctgaatct gatgtaaaag atataataaa
    35101 ttcgagagat aatactttaa aggggtatgg aagaatagaa ttagatataa aagttacaaa
    35161 acagttaata gaaaatatat atacatcaca atatacaaat gtggacaatt atttagaggc
    35221 tattaatgat atgcaagaaa ttttttatta tttaaaaaat gaaactgatg acaagatatg
    35281 tgatgatgag attatagaaa tactgggtga attttatgaa aaattttcag gcaatatgga
    35341 caatgtaaga ggagaagcag acgaatttgc aaagaaattt aaatttggag aagtttaata
    35401 tatgatagga gagattttgt atgttaaata atttaagtaa tattaatata aacgaaaata
    35461 atttaataaa gaaccaatat tccatatctc tacttaagga atgtctaaat tgtaaagtga
    35521 tagatgaaag agaagtatat aatatacaac aagaaatatc tctaatactt atggatttga
    35581 ttaaaaaata tacaaatggt caaagcacat cagtaaaaac agaagttgcc gaaaaactgt
    35641 tgatttcaat atggtatgct attgatgcat atataaataa gcttggagat atagaaaaaa
    35701 gagttgtgga gttgactgct aagaatgtta aaaaagtgta ccttggaggc attgaaatat
    35761 tagaggaaga gttagttagg ctgaaagatt tttataaact aatgatgagt agaaaattgg
    35821 atactaattc aatagcatat aatgatacct tgatggagtt atcaagtttc tttaaaagtt
    35881 atattgtaaa atttgaagct caggacgttc cagtaagtat agattatcca ttggcactag
    35941 atgatatgga tgttaagggt atttattatg tgaagaatta tattgagagt atagatatag
    36001 aaaatagatt ttgtaaccta tttaagaaaa aagatagaga aaaattattt tatgactatg
    36061 gggtgattta taaaatagat tgtgatgtta tacttgtaaa tttatttgaa cttattataa
    36121 ataattgtat tttttctact attttaggaa atgaagtgat taatttagaa atttcaaaat
    36181 atgagtatga atttttagaa aaacaattta aaaatatgta caataatatt actgaagata
    36241 aagttatagg ctctgaagaa agtaatataa ttgaattgat tgaagtagat gatagtttta
    36301 acactgaagt agatagtaat ttggattata atgattttga agaaattaac agagaaatta
    36361 gaataaataa aaaaataact ttactactta tggaagcaat tgaagtatta ataaaaagat
    36421 taagcataga agatgaagat gtaataagat atattaaaaa atatgaaaag atatttattg
    36481 aatctgtgct aagctcaata aaaagtaaca cttttaaaag tatggtaact gtgagtaaaa
    36541 ataaaaatga acacttagaa gactatattg ttgatgatga aagtaaattg gatgatgaat
    36601 cgtttagaga gatattcaat gagatattag atagtacatc tataattgaa aaaataagaa
    36661 taataaagga aaatatcaat gctaaaaaag actttatcga tattcttgaa tctgaatgtt
    36721 tatttggaga agagtattta atgttgtttg catcattaag tgaattagaa ttggcaatat
    36781 tgggtagtgt agtattttat gaagatatta gaatgagaga aattaatata ttagaattta
    36841 tgttaaatga gaaaactgaa actactttat ggaagacaga gtacattaaa tttatgaaga
    36901 ggcaaagtga agataagata tactccatag aagagcatat gaataaaatt aattaacaaa
    36961 aaattgtgaa taaatagaat gttagtatat aatataatct gtaaagcata tcaaaaatac
    37021 atatatttgg aggaaaatta tgaaagagta tatcataaat aatgttgaag aaataagaga
    37081 agaattaatt gatttatcta agaaaatatg ggaaaatcca gaattagcct ttgaagaaaa
    37141 gtatgcaagt agcattcaaa aagaatacct aaagtcaaaa ggatttaaaa ttgaagaagt
    37201 agaaaattta cctactggat ttatagcaag ttttggagag ggtaaacctg tgattggcat
    37261 acttggagaa tatgatgctt taccagagct atcacaatgt gtgagtgcag aaagaaagcc
    37321 attggtagaa ggaaaagctg gacatggatg tgggcataac ctgttgggtg tagcaggtgt
    37381 aggagcagtt gtttctataa aaaaattaat agaagaaaat aaatttaatg gaacaataaa
    37441 atattttggt tgtcctgctg aagaagaagg tggaggaaag acagttatgt gtataaatgg
    37501 ctgttttgat gatgtagatt gtgcatttac atggcatcct tttgatataa atgcaccttg
    37561 gagaggtgga agtctggcaa acttatctgt aaaatttaaa tttaaaggga taacagccca
    37621 tgcagctcaa gctcctcata atggtagaag cgcacttgat gctgttgaaa ttatgaatgt
    37681 tggagctaat tatttgagag aacatgttat tgactctata agaatgcact atgttataac
    37741 taatggtggt ggaagaccta atgtagttcc tggatttgct gaaagttggt actttattag
    37801 aggtaaaaaa gcaagggatg cagaacatgt tcttgataga ttaataaaag tagctcaagg
    37861 agcagcaatg atgacagaaa ctgaaatgga gtataaggtt acagatggaa tatatgacta
    37921 tataccaaac caatgtttga ctgatttagt ttatgacaat atggtttttg ttggttgccc
    37981 taaaaatact cctgaagaag aagaatttgc aaaaaaattg tgtgatactc ttactagaga
    38041 agaaagatta ggtgtagcaa catcattaca aaatgataaa tctattgtag aaagttatat
    38101 acacaaggaa atagttgacg gtgacaaaga taaaggacta gctggttcta ctgatgttgg
    38161 agatgtaagt tatgtaatac cagtggctca atttgcaatg gcagcttggc ctgttggaat
    38221 tgcaagtcat acatggcagt catgttcatc agcaggttca aatataggat tttctgctat
    38281 gataaattca gctaaagtac tggcttgtag tgcttatgat gtatttatga atactaaaat
    38341 tattgatgaa gcaaaaattg aatttgataa atcattagat ggacaaaaat ttaaaccatt
    38401 ggtataattt tggtaataaa atatttctgg ggggaatagc tatgagagaa aaagttgaaa
    38461 aagtgcttga ggaaaagata aaaccagtat tacaaagaga tggtggagat gtagagctta
    38521 tagatgtaaa tgaaaatggt gtggttttag ttagactaca aggggcatgt agtgggtgtc
    38581 caggagcgac tatgacaatc aaagctatta ttgaaaatgt tttggtgagt gaagttccag
    38641 gagtaactca agtattaggt gtttaatagt tagcgtttat taaatgtatg ttatacataa
    38701 gtatttaata aagaagtaaa aaaaatagtg aagcaatata tatgaaaaag tatgataaag
    38761 ataataaaac attatagaaa taatgaaata tttcatatgt atatgaagaa ataagaaaaa
    38821 atagtgaagc aatatatata tgaaaaagta tgataaagat aataaaacat ttatagaaat
    38881 aatggaatat ttcatatata catatgaaaa aatagtaaaa caatatatat atgaaaaagt
    38941 gtgagaaaat aataaaatac tatagaaaca attaaatatt ctatataggt attaagaaac
    39001 atgaaagaaa taatggagta ttatagataa atatgtaaaa gtgtgaaaga aggatgtaga
    39061 taaatttgtc ttatacaaat ttacctacag tatttcttac acacttttta ttttactatc
    39121 caaccatacc aataaacata cctataaagt atggaactaa ccatatagtc catacaacta
    39181 tactaaactt atgaaagaat tttttctttt cctcatcatc tttgtatagg acaaaagtag
    39241 cccaacttgc atgaaaaagc ataagcacaa ttgcaagaag acctgtaata ctatgaaggt
    39301 tttgtgataa agcagatttt acttcaaaag tatggctgtt ggctatgtta ctcattgtaa
    39361 atgttcctaa agtatcaaat ataaacccaa gccaaaatat aattacatgt ttctttttta
    39421 aaatttttgc ctttctttct ccaaatacac ctatagtgta gaaaattaaa gctgatgtta
    39481 taaatactat tgcaagtatt aatttataat tcataattat ctctccttta cttttgccaa
    39541 tatatttcta agatttttta ctaagtcact caattcgttt tcatcacaat ttttaaaaat
    39601 atcatcaaaa ctttcttgca ttttaatttt aaaatttgtg gcaaattcaa atcctgttgt
    39661 tgtaaactta acgtatgtat ttcttttatc attagatttc ttgaattttt ctatatatcc
    39721 aatctgttct aatctgctga ttatgcctga aaccgtacct ttagctaaag acatttcatc
    39781 acatagttgt gatattgtaa gttcttgatt atgagctacc agttttataa ctataatctg
    39841 ttggtgtgtt aatccatttt ctttaaaatt ttcttctact gtgtacatag ttttagaata
    39901 aagttctttt aagagcatgg ctattctaaa acagtcataa ttacttttca ataaaatcac
    39961 ctcttaaatg gtttgcatac aaactaatat aatatacttt tcttatatag tcaacctaaa
    40021 attttataat tttatagaaa ataatgaaga atagaacata aaaattttat tttaggtata
    40081 aataaaataa tttgatgaaa ttttaacaat ttttaaaaag tttgtttaca cagttaataa
    40141 atgatgctaa aattaactca tagataaata tagaaaattt aattttgttt aatgataatg
    40201 gaaccacgaa ggttttaacg ttactttacg tttgctttta tgtggttttt ttattgcaac
    40261 aaaggggggt tggggtttgt taaaggatat actgaaaaag ttgttgcagt ttattttagt
    40321 aatgttttta ttatctttta ttgtttttta catggcaaga cttgctccag gagacccatt
    40381 aatctcttac tatggggatg gagtagaaag aatgagtaca caagaaaaag aaaatgctat
    40441 gaaaaaactt ggtttgaatg aaccaatata cagtcaatat ataaaatgga tttcaaatgc
    40501 atcaaaaggg gaatttggaa tttcatttaa atataaacaa aatgttactt ctgttataaa
    40561 tgatgtctat ataaacacta taatattagg tggttcaggg tatatactta cctttgtgct
    40621 agccttattg ttaggaatat tttgtacatt acatgaagat aagctcattg atagaatcat
    40681 atgcaaatta ggaacaataa ccaactgtat tccatcattt tgggttgcct tagtactcat
    40741 acttatattt agtataaact taagtatact tccaagtagt ggtgcttatt caatgggaga
    40801 agagtccagt atattaagta ggatatcaca tctaatctta cctcttacag tacttatatt
    40861 aagccatcta tggtattaca catatatgat aagaaataaa ttactagagg aaataagaga
    40921 agattatgtg cttttatgta aagcaaaagg tttaaataat agaactattg tttttaagca
    40981 ttgcctgaga aatataatgc catcatatat tagtataatg gccatttcga tacctcatat
    41041 actaggagga acttatgtag ttgaaaaagt tttttcttat cctgggttag gaacactttg
    41101 ttttgaaagt gcaaagtatc atgattataa tatgttatta gttttatgct taataacagg
    41161 ggctttagta gtatttggga atatgttagc tcaaattata aacaataaaa tagaccctag
    41221 aatgaaatat gatagaggtg atactaatga agccacaata tagtgatttt gaaattgtag
    41281 gagaaaatta tattttagta gcagaagatg aacatataga aacaaagaaa accttatcta
    41341 aaaaaattaa agaattacca tatatatcaa ttattatttt atcaatcata gtcattggaa
    41401 gtgtattctc atcttttata atgacacatg aaccaacata catggattta gcaagttcta
    41461 atttagctcc aaataaaact tttttatttg gaacagactc aatgggtaga gatatatatt
    41521 ctatgatatg gtatggagga aaaatatctt tatttatagg actattttcc actgtaatat
    41581 caactgttat aggaattgtt tatggtagta taagtggttc agcatctgaa ctcgtagatg
    41641 atgcaatgat gaggtttaca gaaatcatac ttagtatacc ttcaatatta attataatat
    41701 ttgtacaagc tatacttgga aattcaaatc cagtatctat gtccatagta atagggataa
    41761 caagttggat gaatatatct aagatagtga gaacagaagt tagacaaata agaaatagcg
    41821 aatacatact agcagctaag agcatgggag gaggattctt ctatgtatta aagcaacatt
    41881 tacttccaaa ttttgtggca tcaataatgt tcatggttgt aacaaacata ggcgcagcta
    41941 ttggtactga atcgacactt agctttttag gaattggttt accaatagaa attgtttctt
    42001 ggggaagtat gttatctttg tcagaggaag cattactttc aaatagatgg tggattatac
    42061 tgattccggg tatatttttg gttaccacat tggtgtgtat aacaaatata ggtaattata
    42121 taagaaagaa taataataaa aaatctagta atttataaat cagcatttga gtactaaata
    42181 agcaaatact gaggtatttg aaatatctat ttaaataaat ggggtcaaag ctttgaaaaa
    42241 tattttataa aaagcaaaag ggggagcatc atgaaattaa agaagttaaa agttttaagt
    42301 ttagtaatga tacttagtct aatggcaggt tgttctagtg gaggagacaa agataagaag
    42361 gcagatacgc ctaaagatgg aaaggtactt gtttatggaa gtaatgacta tacaagtata
    42421 aatcctgcac tatatgaaca tggagaaata aattcattaa tatttaatgg attaacagct
    42481 catgatgaaa ataataaggt ggttccttgt ctagcaaagg actggaaatt tgatgaggca
    42541 acaaatacat atacttttaa tttaagagat gatgttaagt ggcatgatgg agagaaattt
    42601 acggcaaatg atgttaagtt tacaatagaa actataatga atccagataa tgcttctgag
    42661 atagcatcaa attatgaaga tataacaaaa attgatgtgg ttaatgataa tactataaaa
    42721 attactttaa aagctccaaa tacagcaatg cttgattatt taacagtagg agtattacca
    42781 aaacatgttt tggaaggtaa ggatatagct actgatgaat ttaatcaaaa accaataggt
    42841 actggaccat ttaaacttga aaaatgggac aaaggtcaaa gtataacact tgttaagaat
    42901 agtgattatt ttgtaaaaga accaggatta gataaagtag tctttaagat tgttccagat
    42961 gataaggcta aggcaatgca attaaaatca ggagaattag acttagctca aataactcct
    43021 aaagatatgt ctaactttga aaaagacgaa aagaatttta aagtaaatat aatgaaaaca
    43081 gccgactata gaggtatatt atacaatttt aattctaaat tctttaaaga taaaaaagct
    43141 aaaggattac caaatgcttt aagctatgca atagatagaa aagcaatagt tgacagtgta
    43201 ttattaggac atggtgtacc agcatattca cctttacaaa tgggaccata taataatcct
    43261 gatatagaaa agtttgaata taatccagaa aaagctaaac aagaaattga aaagctaggt
    43321 tggaaattag gttctgatgg aatctatgaa aaagaaggta ctaagttagc tttcgagata
    43381 acagcaggtg agagtgacca agttagagtt gatatggcta aaatatgtgc acaacagtta
    43441 aaagaaattg gtgttgatgc taaagctgtt gttgtaactg aaacagattg ggctaatcaa
    43501 gatgctcatt taataggttg gggaagtcca tttgacccag atgaccatac atataaagta
    43561 tttggaacag ataaaggtgc aaattacagt gcttactcta atcctactat agataaaata
    43621 cttcaaaaag caagagaaac agaagataaa gatgaaaaat taaagttata taaacaattc
    43681 caagtagaaa tgactaagga tatgccttat acatttattg cttatataga tgcaatatat
    43741 gttggaaaac caaacataaa aggattaaca cctgatacag ttttaggtca tcatggtgta
    43801 ggtatattct ggaacatagc tgactggaca attgaataat actgaaatta attagaaacc
    43861 ttagggataa aaatatatcc ctaaggtttg aatagcatag gggttgatgg tttttggaac
    43921 atttattgga ggttaataat ctatctgtaa gttttaaagt agaagaagga gaagttcagg
    43981 ctgtaagaaa tgtatctttt aatctaaaaa aaggtgagac attggctata gttggagaat
    44041 ctggttgtgg gaaatctgta ctgtgtaaga gtttgatgag gatactccca tacaatggat
    44101 atattaaaaa tggagaggtt ttacttaaat caagtgattt ggttaagaag tctgaaaaag
    44161 agatggaaga tataagagga aagaatatat caatgatatt tcaagaccct atgacatctc
    44221 tcaatccaac tatatcaata ggaaagcaaa ttgcagaagc tgtaatcatt catcaaggta
    44281 taagtaaaag tgaagcaaaa aagagagcca tagagcttat tgaattggtt ggaattgata
    44341 atcctgagaa aagatttaaa caatttccac atcatttttc aggtggaatg agacaacgta
    44401 tagtaatagc tatagcactt gcctgtaatc cagatgtatt gattgctgat gaacctacaa
    44461 ctgctttaga tgtaactata caagctcaaa taattgattt aataaaggat ttacaacata
    44521 aaataggttt gtcaataata tttataactc atgatttagg agtagttgca actatagctg
    44581 atagaatagc agttatgtat gcaggaaaga ttgtagaaat aggaactgta gaggatatat
    44641 tttatgaccc tagacatcca tatacttggg ggttgttggg ttcattacct actttagatt
    44701 cacaggatga atatttatat aatatacctg gaatgcctcc aaatttattg aacccaccaa
    44761 aaggagatgc ttttgcaata agaaataaaa atgcacttaa gatagattat gaaaaagagc
    44821 caccaatgtt taaaattaat gatacacata gtgcagcaac ttggttattg cacccagatg
    44881 cacctgaagt agatgtacca gttagagtaa attgtggaag ggtgatttct aatgaataga
    44941 gaaaaaatac tggaaataaa aaacttaaag caatattttc atttggacaa aagtactact
    45001 gtaaaagcag ttgatgatat aagttttgat atatataagg gtgagatatt tggattggta
    45061 ggagagtcgg gttcaggaaa atctacaact ggtaaaacta taataagact acatgaatct
    45121 actggtggag aagtaattta caaaggaaat tgcatatcag ataaaaaaac gtacaaattt
    45181 ataaaaaaag atgtaaacaa aagtatgcag attatttttc aagattctac ttcatcatta
    45241 aatccacgta tgacaattgc agatataatt tcagagccac taaaaattca aggtatctgt
    45301 aaaaataaaa cagatagaat gaataaagta tatgagatgc taaagctagt aggacttgat
    45361 agaagttatg caaataagta tccatctgat ttttcaggtg gacaaagaca gcgtattggc
    45421 atagcacgag ctctttcagt agacccagaa tttataatag ctgatgagcc tatagcttct
    45481 ttggatgtgt ctatacaagc acaaatagta aatttattta aaaaattgca acaagaaaag
    45541 aatttaactt gtttatttat agctcatgat ttatctatgg tgagacatat tagtgaccgt
    45601 ataggagtta tgtataatgg aaaattagta gaattggcag attcaaatga attgtataat
    45661 aatcctattc atccatatac aaaatcacta ctttctgcaa ttcctgttcc tgaccctaga
    45721 tatgctaagt caagaaatag aatagagtat aattctaatg gatatgattg ttcaaatgaa
    45781 aagtcattaa gttggattga ggtttcagat ggacattttg tatattcttc taaatcagaa
    45841 ataaataaat atcaacaaaa tttaaaagtt gtttagtata aaaatacttg aacaggtatt
    45901 tatttgaaat acaaactaaa accctatttt tggagaaatt ttaaaactac tccaaaatag
    45961 ggttttttat ttctactttt caaatataat aaattcaaat ataataaaat taaaaacaag
    46021 taaagtatgt tgttaagaaa gtgctataaa attaaaaata gtaatgtaga aaaaatgagc
    46081 aataatctta ttttttctac aataattttc tataatttaa agtaaggaaa gtttgataaa
    46141 ttataacctt agttaaagaa taagagttta ttaaaaaata aagtgtgtat aggggtttta
    46201 gttaataaaa tattataatg taggggagct tgtaatgata tataaaaaag ataaagtaat
    46261 gaaagtttta gaatcatcat atagggaaaa tgaaaaaggg attacagcta accaattatc
    46321 agaaaagaca ggaattgcaa gaaacaatat aagtacatat ttaaaccaat tattcaatga
    46381 gggtatggtt acgaagataa aaggaagacc tgtgtattat atccctgcaa atcttaacct
    46441 taacaagaat gaattgcttg atacacaaag tgagttgctt ggcataaaaa aaggaacaaa
    46501 agtagaaaac aaaataaaaa aagatagttt tgaaacacta ataggtaaag aacatagttt
    46561 aagacctata atagaaaaat gcaaggtcgc aatgctctat cctacaaatg gacttcatac
    46621 tattatctat ggagaaacag gagtaggaaa gagtatgatt gctagatata tgtacgatta
    46681 ttctatagat agtggaatta gaaaaaataa agctcctttt gtaacattta attgtgctga
    46741 ttatgctaat aatgcacaat tacttatggg acatattttt ggagtagaac aaggagctta
    46801 tacaggtgca gaatcttcca gaaaaggtct tttggaaatt gcaaatggag ggatactatt
    46861 tttagatgaa attcatagac taccagcaga aggtcaagaa atgttgttta cttttataga
    46921 taagggaaca tttaaaagac taggagatgc aacgaaagaa agagattcta aggtattaat
    46981 aatatgtgct actacagaaa atccgacatc aacactatta gatacattca atagaagaat
    47041 accaatgaaa atagaaatac catcactgag agataggaca atgattgaaa gaatggaatt
    47101 agtcaaaaat tttttcaagg aagaatcaaa aagtatttct atttcaataa aagtacataa
    47161 agaaataatt aaatcactat tactttatga ttgtaaaaat aatgtaggtc aacttcaaaa
    47221 tgatataaaa ttagctgttg caaatggata cctaagatat aaaagagata ataaagaacc
    47281 agtttatata gagaaaaaat attttgataa atctataaat gaagtgagtg atgattatag
    47341 agagaagtct attaaagcag atgaagttat aacacatgga atagaatatt ttgtatttac
    47401 gtcattagga gaagaagaag ttattaacta caatgatata aatattatta atgcagtaaa
    47461 tactaatctt aataaatctg tagaaatgaa tatagcagat gcttctctat ttaataatgt
    47521 taagtttaaa agcatgtgtg aatcaataag acaaattatt aaagaagatg aagaaataga
    47581 tataagtgaa tcaatattta agtcaatggc aatttatata aaaagcgtat tggataaaag
    47641 tagctctaca aacaacatag atttgaatca aattagaaga aataacagaa gtgaatttaa
    47701 aacagcacta aaaatagttg gaataattga aaatgaattt gatttatttt tatctataga
    47761 aactgtagca tatattacat tattcatagt acaagctaaa caagatgaac aaaataaaat
    47821 agtgaataac attaatatta tagttgctat gcatggagaa actacagcaa gtaatggtaa
    47881 aagcagttgg tgatattatt ggaaagtgca gtgctatttc tttcgatatg aagttagata
    47941 aaagctatga tgaagttatt gtagatttta aaaatttaat taataatata gacaataaag
    48001 atgtattact atttacagat atgggttcat taaactcctt tgatgaaatt ataaagaaag
    48061 aaaaaaagtg tgaagtaaga gtaattccta tggtgacgac attaactgtg ttagaagcag
    48121 ttcagaaggc caatatggga ttacctttaa atgacgtata caactctatc actaacacga
    48181 gaaagtatta ttttggaact aatgaaattc aaaataaaga aaatctgtct aaaacaatta
    48241 ttatagcatc acatgtttca gaaggtgtag ataataaaac aagaaaaata ttagaagaaa
    48301 agatgagtag gtacttagat ggaatagata ttatatctgt cccatataaa acagaaaaag
    48361 atttaagttt aaatattaca aaacttaaag aaagtagtaa tattgttgca gtaatcaatg
    48421 aacaaagaat taatattaga ggaattgact atattagcaa aaaagatata gataaggatg
    48481 aaaatatcaa taaattaaaa aatattataa agataagtat aggttatgat gatgtagttg
    48541 aaggtttaaa gacatcctta aaaagctcta attataatag gatatttaaa gatataaaat
    48601 atgtgtctga tgaattgttt ttagtattta atatagaaaa gaaatacgat aaagtaattg
    48661 gacttatgat gcatttagct ttcatggtag atgggttgat tggaaatact agagaaattg
    48721 aaaagcttga taaagaaaaa acacttgatt atcacaagag cctaagcaaa ataaaagata
    48781 tagtatctca attagataaa aaatataata tagaaattaa tgaaaaagag tgctatcaaa
    48841 tattattaat attagaatat gcagaaataa tcgaaaaaga ttaccaatga gtataaaaac
    48901 ttatttggta atttttttta gtctaaaata atattaatat tagacacata taaatatact
    48961 tagacaaagt atctaagttt tgatgataaa aatgattgaa ataacagtgt tttaaagtgt
    49021 ctaaaaaaac tggcataata cttgcttata ttattaaatg agtaatataa ataattaaaa
    49081 tttaaataaa aaggagtaaa taatatgcaa gataaaatat taagagataa aaaggcaaat
    49141 gacccatggg caagaatgac tacaagaaac ggatttagtg cagacgaaat aatatcgtca
    49201 tttcaaaaag ctataagaag aaatatggtg gaagaagctt gtgaatttgc ttatgaacta
    49261 tatatatcat caccagaatt agaagataag ctatggagaa ggatacttac aatatcagtt
    49321 gaagatatag gaatggggga cccatcagct gtaataatca taaataattt aaatcaagta
    49381 agaaaggaat attcatatgc agatggagac agacctttat ttttcataca tgcaataaga
    49441 tatctttgtg catgtgagaa agatagatca agtgatttat taaaaaatat aattataaaa
    49501 ggatttgcaa tgggtaaagt tccagaagtt atggacgtgg cacttgacaa gcatacatat
    49561 agaggattag aaatgggaag ggattcattc cactttttaa atgaagctag tatagtaata
    49621 ccagaaaaag aagtagataa tgattataaa gaaagatact taaaaatatt agaaaaatat
    49681 aagcaagaag aagttataga tacggctttc aagtttaatc attggcagta ctaagaatta
    49741 gagtaaatat taataagaat taaaataaaa taataagaat taaagtaaaa actgatagga
    49801 attagagtaa gaattggctg ataaaaaatt agaaaataaa agaggttaga tattgtagga
    49861 gggtaattat gaatatttta ttagtatgtg caaatggagc ttcaacaggt gtacttgtgg
    49921 aaaagatgaa aagtttttgt agtgagcatg aaaagttaaa aactaaaact ataaatatag
    49981 aggcaacatc attcgaaaat ttaaaatcat acattgaagc taatgataca gatgtagtac
    50041 ttgtagcacc acaaattagg ttcaaggaag atgatgtagt tgaagcttgt aagaattaca
    50101 aaatagcagt tggtttaata gatactaagc attatggtcg tatggatgct ccttctgtta
    50161 tgaagtcggc aatcgaatta tataaaaata gataattggg gggaataaaa atggaagcac
    50221 ttcaaaataa tttaagaaga tttcttctac caatagcaca aaagattgaa aaacaaaggc
    50281 atcttcaagc tataaaagaa ggtatgataa gcattacccc aataataatt gttggttctt
    50341 taagtatgtt atttatggca ttaaacaata tgttacctga aggaagtgca aaaacattat
    50401 tatctgaaaa tatggataca ttacttatcc ctaataaatt tactatgagt ttattatcta
    50461 tatattcagc attttttata gctcaggcac tagctaaaaa atataatctt aatcatgtag
    50521 aaataggtat gacagcagtt gttgcacaac ttgttgtatg tggacaagta gtagatggtg
    50581 tattagatac aagttattta gatgcacaag gtttatttgt aagtatatta gtagcacttt
    50641 tagtagttga tattactaag tttatgaatg ataaaaatct cgtaattaga tttccaaaag
    50701 aagtacctag tgtagtaaat aaaagtttta gaaatttaac tccaatgata gtatgtataa
    50761 tgttatttac agcaatagca gcaataacta aaaatgtctc aggtcaacca ttaccagcta
    50821 ttataatgaa tttcttagca ccagctataa gcagtgtaga taatgttttt gcagtaacta
    50881 taatactatt tataactcaa ttattatggt tctttggcct tcatggagca gcaataactt
    50941 caagtatatg gatgccaata gcagctacat atatggcaga aaatgcaaca ctgatagcag
    51001 caggaggaga cccaaaatat gtgtttacaa taggatttta ctatggtttc ttgcaagtaa
    51061 ctggttcagg gataactttg ggattagtgt accttatgtc aagaagtaag tgtaagagtt
    51121 ttaatagtat gggaaaagtt gtgattttac catcactatt tggaataaat gagccagtaa
    51181 tatttggaac accaatagtt atgaacccat atatgtttgt accatttgta tttggacctg
    51241 tattggttgg agcacttaat tttatggcat taaaagtagg attagtagga ttacctattg
    51301 cagaaccacc aggcttttta cctccagggg ttgcagcatt tttgatgaca ttagattgga
    51361 aggcaatcgt attagtattt gcaagtataa tacttatgac cttgatatat tatccattct
    51421 ttaagataat ggagaaggaa gaattaaata aaaatgcaga attagctaca acattagatg
    51481 atgatagctt tgatttttaa caagtcatta gaaggagaag gatatggtac ctgaaatgga
    51541 agaaaaagta tttagtataa tatcagaagc aggagatgct aaaagtgatg taatgatgtc
    51601 tcttaaggaa ataaaaaaag gtgactacaa taaagcaaaa caccttttaa attcagcctc
    51661 agagaaaata caaacagcat caaagcatca tttagaatta ctatcaaatg ctatgaatag
    51721 tgaagatagt gggactgatt ttttagttgt gcactctgaa gaccattatt caaatgcatt
    51781 atttgctcat tctttagtat ctgaacttgt agatatattt gagatgatgg acccaagaat
    51841 taataagaat taaatttctt gtaaatagtg cttgaagttt ggctatagtt tattaaatca
    51901 atagatttaa taaatgaact cccttttact ttaaagtact agtatttata aatttattta
    51961 taaatacagt aaatttttaa atagaaaagg agatattatt atggaaaaat tttcagtagt
    52021 aatagctgga ggaggaagta catatacccc agaaatcata ttaatgttgc tagataactt
    52081 agataggtta ccattaagag caatcaaatt atatgataat gatgaagaaa gacaaaataa
    52141 ggtagctaaa gcttgtgaaa tactaataaa ggaaaaggac cctaatatag agtatttagc
    52201 aacaacatgc cctaaagagg cttatacaga tgtagatttt tgtttagctc atataagagt
    52261 tggaaaactt gaaatgagag aactagatga aaagatacct ctaaaacatg gagtagttgg
    52321 tcaagaaact tgtggaccag gtggaatagc ttatggaatg aggtctatag aaggtgttct
    52381 tgaaaacata gattatatgg aaaagtattc tccaaattgt tggatgttaa attattctaa
    52441 tccagcatca ataatagcgg aagctgttag aaggttgaga cctaattcta gggtaataaa
    52501 tatttgtgat atgccaatag gtatggaaca taatatagca aggattgcag gactcaaatc
    52561 tcgtaaacat atggatataa gatactttgg attaaatcac tttggttggt atacttcaat
    52621 taaagataaa aaaggaaatg aacttttacc aaaattagta gaacatataa aagaatatgg
    52681 ttttataaat ggtgaagaag gaatgaaaaa tgacaagaaa gatagttggt ttgaaactaa
    52741 tttatttact aaagaaatag taaaaacaga tttaactaca ataccaagta gttacttaaa
    52801 gtactatcta tttccagatt atgttgtaaa tcattctgat gtaaattata ctcgtgccaa
    52861 tgaagttatt gatggaagag aaaaagaagt ttttggttca tgtgcattga tagaaaagca
    52921 aggacattct aagggcagtg ggcttaagat aggaatacat gcagagttta tagtggattt
    52981 agctacagct ctagctttta atacacagga gcgtatgtta ttgatagttc aaaataatgg
    53041 agctatagaa aatatacaag atgatgctat ggttgaaatt ccatgtatag ttggaaaaga
    53101 cgggtatgaa ccattatcaa tagggaaaat accaacattc caaaaaggtt taatagaaca
    53161 acaattagca gttgaaaaat tagtagttga tgcttggatt gagaagtctt ataataaatt
    53221 gtggcaagca cttatgctat ctaagacagt tcctagtgca acagttgcaa aagaattact
    53281 agatgattta atagaagcaa ataaaaatta ttggccaaaa cttaattaag aataatatat
    53341 ttttaaagtt cctacagttt agtacatagc ctagttaatg tatttgtagg gactttattt
    53401 atttacaaat gagatacaag tggcaaacat agtgtatttt gcagttagga aagtaggttt
    53461 agttacttgt tagttggatt aaatttaggt acaataattt actatcaatc aatagaaata
    53521 accagagaag ttgcataata aaaaatttac acttttaaca ggatttatgt aggtgtttat
    53581 agaaataaat aaatatgtac ataaatacat aattattttt aagtctaaaa tacaagaatg
    53641 gagagatggc tatgaagtgg agagattatg ctgatgatgt aaatttattt gaggattttg
    53701 ataaaactat caagcctcta actgacgaac aaagaagaaa aaatataaac actttaattg
    53761 catatttttc aaaagaagtt ccttctaaaa tatacaattt atcaaatgat gaaattaagc
    53821 ctagagatat acttagagga cttttgaatg tatatccacc aaaagaaatt gctccagaga
    53881 tacttaatat gttacataat ttattgttga ttgaatgtga agaaagagag ttagtagatg
    53941 taaatgatat agaagaagta gaggaaggga ttgccatatg gagaggtaat ataacgaatt
    54001 taagagcaga tgcaatagta aatgctgcaa ataacaagtt attagggtgt ttacaaccat
    54061 tacatctatg cgtggataat gagatacatt catgcgcagg tcctagactt agagaagatt
    54121 gtgacaaaat aataaaaaaa caagggcatt tagaatatac tggagatgct aagataacta
    54181 gaggctattg tttacctgca aaatttgtag tacatactgt tggacctatt gtatctggtg
    54241 gtcagccaag taaagagcaa gaaaagcaac tgcttcattg ttataaatca tgtttaaata
    54301 caataaaaga aattgatgaa ataaaaaata ttgttttttg tggaatttct acaggtgtat
    54361 ttggttatcc aaaaaaagag gcagctaatc ttgctgtaag tagagttaga ctatggctaa
    54421 aagaaaatcc tgaaaaaaat ttaaaagttg tatttaatgt gtttactgaa gaagaagaag
    54481 agaaatatag aagaatattt aaataatatt atgtaacaga atcctaaaaa tacttattat
    54541 ataatacaaa ttcattgtat aagataataa tttacaagta gtaaaaatta ataaaatata
    54601 atttgtattt ttaataaata agtaggtata atagaaatta taagaatagt ataatcaatt
    54661 atgctattac ttagaaaata taa
//
LOCUS       DAMJVX010000404        34464 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.guided.021, whole genome shotgun sequence.
ACCESSION   DAMJVX010000404 DAMJVX010000000
VERSION     DAMJVX010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.guided.021"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            <1..1085
                     /locus_tag="PC407_003815"
     CDS             <1..1085
                     /locus_tag="PC407_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2499178.1"
                     /translation="GVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVD
                     PKAERGRWKETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLT
                     GISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDF
                     AHLLFSNGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFA
                     LDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLD
                     EKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRV
                     GVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            1088..3265
                     /locus_tag="PC407_003816"
     CDS             1088..3265
                     /locus_tag="PC407_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2499179.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            3262..4263
                     /locus_tag="PC407_003817"
     CDS             3262..4263
                     /locus_tag="PC407_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDF2499180.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            4260..5192
                     /locus_tag="PC407_003818"
     CDS             4260..5192
                     /locus_tag="PC407_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2499181.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5437..5553
                     /locus_tag="PC407_003819"
     CDS             5437..5553
                     /locus_tag="PC407_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDF2499182.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5569..7488
                     /gene="tet(M)"
                     /locus_tag="PC407_003820"
     CDS             5569..7488
                     /gene="tet(M)"
                     /locus_tag="PC407_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDF2499183.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7607..7774
                     /locus_tag="PC407_003821"
     CDS             7607..7774
                     /locus_tag="PC407_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDF2499184.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(7834..8187)
                     /locus_tag="PC407_003822"
     CDS             complement(7834..8187)
                     /locus_tag="PC407_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2499185.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            8692..9114
                     /locus_tag="PC407_003823"
     CDS             8692..9114
                     /locus_tag="PC407_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2499186.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            9111..9341
                     /locus_tag="PC407_003824"
     CDS             9111..9341
                     /locus_tag="PC407_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2499187.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            9802..10005
                     /locus_tag="PC407_003825"
     CDS             9802..10005
                     /locus_tag="PC407_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2499188.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            10087..11304
                     /locus_tag="PC407_003826"
     CDS             10087..11304
                     /locus_tag="PC407_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2499189.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(11732..13462)
                     /gene="lpdA"
                     /locus_tag="PC407_003827"
     CDS             complement(11732..13462)
                     /gene="lpdA"
                     /locus_tag="PC407_003827"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2499190.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEVPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            complement(13629..14675)
                     /locus_tag="PC407_003828"
     CDS             complement(13629..14675)
                     /locus_tag="PC407_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDF2499191.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYSGKIRKCDVEKLTAKETIVSNNTSKYSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            complement(14688..15674)
                     /locus_tag="PC407_003829"
     CDS             complement(14688..15674)
                     /locus_tag="PC407_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDF2499192.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            complement(15699..16661)
                     /locus_tag="PC407_003830"
     CDS             complement(15699..16661)
                     /locus_tag="PC407_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDF2499193.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            complement(16699..17652)
                     /locus_tag="PC407_003831"
     CDS             complement(16699..17652)
                     /locus_tag="PC407_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDF2499194.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            complement(18097..19470)
                     /locus_tag="PC407_003832"
     CDS             complement(18097..19470)
                     /locus_tag="PC407_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2499195.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNYIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMFFYKLDKEYSTI
                     LNELNTK"
     gene            complement(19567..21213)
                     /locus_tag="PC407_003833"
     CDS             complement(19567..21213)
                     /locus_tag="PC407_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDF2499196.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYIHSSGFDASILHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWRPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNVLGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIADESKDVD"
     gene            complement(21235..22401)
                     /locus_tag="PC407_003834"
     CDS             complement(21235..22401)
                     /locus_tag="PC407_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HDF2499197.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNIVLDTHQYLMLAESDGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            22618..23463
                     /locus_tag="PC407_003835"
     CDS             22618..23463
                     /locus_tag="PC407_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2499198.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCTYQDTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSGKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            complement(23649..25826)
                     /locus_tag="PC407_003836"
     CDS             complement(23649..25826)
                     /locus_tag="PC407_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499199.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDMSTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSENGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVVFVELGDTYRLDLYKPNLNEKTYESVKDNIEKNIDVYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVLFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(25955..27049)
                     /locus_tag="PC407_003837"
     CDS             complement(25955..27049)
                     /locus_tag="PC407_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDF2499200.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            complement(27138..28208)
                     /gene="disA"
                     /locus_tag="PC407_003838"
     CDS             complement(27138..28208)
                     /gene="disA"
                     /locus_tag="PC407_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HDF2499201.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            complement(28211..29584)
                     /gene="radA"
                     /locus_tag="PC407_003839"
     CDS             complement(28211..29584)
                     /gene="radA"
                     /locus_tag="PC407_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDF2499202.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDENIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            complement(29764..32211)
                     /locus_tag="PC407_003840"
     CDS             complement(29764..32211)
                     /locus_tag="PC407_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDF2499203.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            complement(32198..33223)
                     /locus_tag="PC407_003841"
     CDS             complement(32198..33223)
                     /locus_tag="PC407_003841"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDF2499204.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            complement(33239..33733)
                     /locus_tag="PC407_003842"
     CDS             complement(33239..33733)
                     /locus_tag="PC407_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDF2499205.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            complement(33740..34204)
                     /locus_tag="PC407_003843"
     CDS             complement(33740..34204)
                     /locus_tag="PC407_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDF2499206.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
BASE COUNT        10737 a         6379 c         4751 g        12597 t
ORIGIN      
        1 aaggggttaa gggttcagta accaatgcgt tagcgtcggc ttttgttggt tcgctgggtg
       61 gtggtaaatc ctttgcgaat aaccttatcg tctattatgc ggtgctttat ggggcacaag
      121 cagtgattgt agacccaaaa gcagaacgtg gcagatggaa agaaaccttg ccagagattt
      181 cccatgaaat caatatcgtc actctgactt ctgatgagaa aaacaaaggc ttacttgacc
      241 cttatgtgat tatgaaaaat cccaaagatt ctgaatcact ggctattgat attctgacat
      301 tccttacggg gatttcctct cgtgatgggg aacgcttccc aatccttaga aaagccattc
      361 gtgcagtaac caatagtgaa gtacgagggt tgatgaaagt gattgaggaa ttacgggttg
      421 agaatacgcc actaagtacc agtatagccg accatatcga aagttttaca gactatgact
      481 ttgcacattt attattcagt aatggttatg tggagcagtc tatcagctta gaaaaacaac
      541 tgaacattat acaggttgcg gacttggtac ttcccgacaa ggaaacttcc tttgaggaat
      601 ataccactat ggagctttta tccgttgcta tgctgattgt cattagtacc tttgctttag
      661 actttatcca tacagaccga agcattttca agattgtaga tttagacgaa gcatggagct
      721 ttttacaggt agcacaagga aaaacactat ctatgaagct ggttcgggct ggtcgtgcta
      781 tgaacgctgg ggtatatttc gtgacccaaa atacagacga cctcttagat gaaaaactga
      841 aaaataacct cggcttaaaa tttgcatttc gttccactga ccttaacgag attaaaaaga
      901 ccttagcctt ttttggtgta gacccagagg acgaaaacaa tcagaagcga ttgcgtgatt
      961 tggaaaacgg gcaatgcctt atcagtgatt tatatggtcg tgtcggtgtg atacagttcc
     1021 accctgtatt tgaagaactg ctccatgcct ttgataccag accacctgtg cgaaaagagg
     1081 tgtaaatgtg aaaccatcaa tagtaaacag aataaaatca aactggacgc tgaaacgtct
     1141 aggtaaagtg gcaatgacag tggctttcac acttgtgatt gccatttttc ttttagccat
     1201 gctgggaacg gtggttcaag ctgcgggctt ggtagatgat acggtcaatg tggcaaatga
     1261 atacagccga tacccacttg aaaactatca actggatttt tatgtggata atagctgggg
     1321 ctggcttccg tggaactggt cggacgggat tggaaaacag gtcatgtatg gactatatgc
     1381 cattaccaat tttatttgga caatcagttt gtatgtttcc aatgcgacag gttacttagt
     1441 acaggaagcc tattccttag acttcatttc cgctacagca gattccattg gtaagaatat
     1501 gcagacctta gctggtgtga gtgcaaacgg attttcaaca gagggtttct atgttggatt
     1561 cctcttactc ttgattttgg ttcttggggt ttatgttgcc tatacgggac tgataaagag
     1621 agaaaccaca aaggcaattc atgccattat gaattttgtg ctggtgttta tcctatcggc
     1681 ttcctttatt gcctacgctc ccgactacat taaaaaaatc aatgactttt catcagacat
     1741 cagtaatgcc agtttatcac ttggcacgaa gattgtcatg ccccattccg atagtcaagg
     1801 caaggacagc gtggacttaa tcagagatag cctgttttcc atacaggttc agcaaccgtg
     1861 gctactgctt caatacaaca gttcagacat tgaaagtatc ggtattgacc gtgtggaaag
     1921 cctgctctcc accagcccag attccaacaa tggcgaagac agagaaaaaa ttgttgcgga
     1981 agaaattgaa gacagaagca ataccaatct aaccattaca aagaccatta accgtttagg
     2041 tacagtcttc ttcctatttg tcttcaatat tgggatttcc atatttgtat tcctattaac
     2101 aggaatcatg attttctcgc aggtactttt tatcatctat gctatgtttc tgcctgtgag
     2161 ctttatttta agcatgattc catcatttga tggtatgtca aaacgagcca taacaaagct
     2221 ctttaatacc attttgacac gagctggaat cacattgatt attacgacag catttagtat
     2281 ttcaaccatg ctctatacct tatcggctgg ttatccgttc tttttgattg cttttctaca
     2341 gattgtgacc tttgcaggaa tctacttcaa gctgggcgat ttaatgagta tgttttctct
     2401 acagagtaac gattctcaaa gtgtgggaag tcgtgtgatg agaaaacctc gtatgcttat
     2461 gcacgctcac atgcaccgtc tacagcggaa acttggacgt tccatgacta ctctaggggc
     2521 tgggtctgcc attgttacag gtaaaaaagg acagtcgggt tcggggagtt ctgcaaggac
     2581 acaagcagat cactcccgac cagacggaaa ggaaaaatca acacttggaa aacgtatcgg
     2641 tcaaaccatc ggtacagtag ctgataccaa agacagaatg gtagacactg ctagtggttt
     2701 gaaagaacag gttaaagatt tgccgaccaa tgcaagatat gcagtatatc aaggaaaatc
     2761 caaagtaaaa gagaatgtcc gtgatttaac cagtagtatt tctcaaacca aagcggacag
     2821 agccagtgga cgcaaggaac agcaggaaca aaggcgaaaa accattgcga agcgtcgctc
     2881 tgaaatggaa caggtcaaac agaaaaaaca gcctgcttct tctgttcatg aaagaccgac
     2941 tacaagacaa gaacaatatc atgatgaaca gacctcaaaa cagtctaata ttcagacttc
     3001 atataaggaa tctcaacaag ccaaacaaga gcgtccagca gttaagtccg atttttcaag
     3061 tccaaaagtg gaacgccaag gcaataccgt tcaagaaaaa accgttcaaa agccagcaac
     3121 ttcaaccact acagcagata gaacttcaca acgtccaatc acaaaagaac gtccgtctac
     3181 tgttcaaaga gtaccactac aaaatacaag aagtagacca ccaatcaaaa ccgccaccat
     3241 taagaaagtc ggtaagaaac catgaagttg aaaactttag tgattggtgg ttctggatta
     3301 ttcttgatgg tcttctcact gcttctgttt gttgccattt tattttcaga tgaacaggac
     3361 agcggaattt ccaatattca ttatggaggt gtgaatgttt ccgcagaagt gctggctcat
     3421 aagcctatgg tagaaaaata tgccaaagaa tatggcgttg aagaatatgt caacatactt
     3481 cttgcgatta tacaggtgga atcgggcggt actgcggaag atgttatgca gtcctcggaa
     3541 tccctcggtc ttccacctaa ttcattgagt acagaagaat ccattaagca aggtgtgaag
     3601 tatttcagtg aattattagc cagtagcgaa aggctcagtg tagatttaga atcggttatc
     3661 cagtcctaca attatggtgg tggtttctta gggtatgtgg ctaatcgtgg aaataaatat
     3721 acctttgaac tggctcaaag tttctcaaaa gagtattcag gtggcgaaaa agtgtcttac
     3781 cccaatccca tagccatacc tatcaatggg ggctggcgat acaactatgg caatatgttt
     3841 tatgtgcaac tggtaacgca gtatcttgtc acaacagagt ttgatgatga tacggtacaa
     3901 gccatcatgg acgaagcact gaaatatgag ggctggcgat acgtttacgg tggagcttcc
     3961 ccgactactt cttttgattg tagcggactg acacaatgga cgtatggaaa agctggaatt
     4021 aacttaccac gaaccgcaca acagcaatat gatgtgaccc agcatatccc actatcggaa
     4081 gcacaagctg gcgatttggt tttctttcat tctacctata acgctggctc ttatattact
     4141 catgttggga tataccttgg caataaccgt atgtttcatg caggcgaccc aatcggttat
     4201 gccgacttaa caagccccta ctggcaacag catttagtgg gagcaggacg aatcaaacaa
     4261 tgagaaagga agatttaatg atgaaattta gaaaaaatca gaataaagaa aaacagatac
     4321 caaaggaaaa gaaacctcgt gtctataagg tcaatcctca taaaaaggtt gtgattgcct
     4381 tgtgggtact tttagggctt agtttcagct ttgcgatatt caagcacttt acagctatag
     4441 atactcatac tattcacgaa acaactatca tagaaaagga atacgttgat actcatcatg
     4501 tagaaaattt tgtagagaac tttgcgaaag tctactattc atgggagcaa tccgataagt
     4561 ccattgataa tcgaatggaa agtctaaaag gctatctgac agatgaactt caagctctca
     4621 atgttgatac agtacgcaaa gatattcctg tatcgtcttc tgtaagagga tttcagatat
     4681 ggacggtaga gccaactggc gacaatgagt ttaatgtaac ctacagtgta gaccagctca
     4741 ttacagaggg agaaaataca aagaccgtcc actctgctta tatagtgagt gtctatgtag
     4801 atggttctgg aaatatggta ctggttaaga atccgaccat taccaacata cctaagaaat
     4861 caagttataa accaaaagcc attgaaagtg aggggacggt tgattccatt acaaccaatg
     4921 aaatcaatga gtttttaacg acgttcttca agctctatcc tacagcgaca gccagtgaac
     4981 tttcctacta tgtgaatgac gggatattaa aaccaatcgg aaaagagtac atctttcaag
     5041 aactggtaaa tcctattcac aatcgtaagg ataatcaagt cacggtatcg ctgacagtgg
     5101 agtatatcga ccagcagacc aaagcaacgc aggtatctca atttgatttg gtacttgaaa
     5161 agaacgggag taattggaag attatagaat aacaaatatt ggtacattat tacagctatt
     5221 ttgtaatcac gtactctctt tgataaaaaa ttggagattc ctttacaaat atgctcttac
     5281 gtgctattat ttaagtatct atttaaaagg agttaataaa tatgcggcaa ggtattctta
     5341 aataaactgt caatttgata gtgggaacaa ataattggat gtcctttttt aggagggctt
     5401 agttttttgt acccagttta agaatacctt tatcatgtga ttctaaagta tccggagaat
     5461 atctgtatgc tttgtatgcc tatggttatg cataaaaatc ccagtgataa gagtatttat
     5521 cactgggatt tttatgccct tttgggcttt tgaatggagg aaaatcacat gaaaattatt
     5581 aatattggag ttttagctca tgttgatgcg ggaaaaacta ccttaacaga aagcttatta
     5641 tataacagtg gagcgattac agaattagga agcgtggaca aaggtacaac gaggacggat
     5701 aatacgcttt tagaacgtca gagaggaatt acaattcaga caggaataac ctcttttcag
     5761 tgggaaaata cgaaggtgaa catcatagac acgccaggac atatggattt cttagcagaa
     5821 gtatatcgtt cattatcagt tttagatggg gcaattctac tgatttctgc aaaagatggc
     5881 gtacaagcac aaactcgtat attatttcat gcacttagga aaatggggat tcccacaatc
     5941 ttttttatca ataagattga ccaaaatgga attgatttat caacggttta tcaggatatt
     6001 aaagagaaac tttctgccga aattgtaatc aaacagaagg tagaactgta tcctaatatg
     6061 tgtgtgacga actttaccga atctgaacaa tgggatacgg taatagaggg aaacgatgac
     6121 cttttagaga aatatatgtc cggtaaatca ttagaagcat tggaactcga acaagaggaa
     6181 agcataagat ttcagaattg ttctctgttc cctctttatc atggaagtgc aaaaaacaat
     6241 atagggattg ataaccttat agaagtgatt acgaataaat tttattcatc aacacatcga
     6301 ggtcagtctg aactttgcgg aaatgttttc aaaattgaat atacaaaaaa aagacaacgt
     6361 cttgcatata tacgtcttta tagtggcgta ctgcatttgc gagattcggt tagaatatcg
     6421 gaaaaggaaa aaataaaaat tacagaaatg tatacttcaa taaatggtga attatgtaaa
     6481 atcgataagg cttattccgg ggaaattgtt attttgcaaa atgagttttt gaagttaaat
     6541 agtgttcttg gagatacaaa actattgcca cagagaaaaa agattgaaaa tccgcaccct
     6601 ctactacaaa caactgttga accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc
     6661 cttttggaaa tctcagatag tgatccgctt ctacgatatt acgtggattc tacgacacat
     6721 gaaattatac tttctttctt agggaaagta caaatggaag tgattagtgc actgttgcaa
     6781 gaaaagtatc atgtggagat agaactaaaa gagcctacag tcatttatat ggagagaccg
     6841 ttaaaaaatg cagaatatac cattcacatc gaagtgccgc caaatccttt ctgggcttcc
     6901 attggtttat ctgtatcacc gcttccgttg ggaagtggaa tgcagtatga gagctcggtt
     6961 tctcttggat acttaaatca atcatttcaa aatgcagtta tggaagggat acgctatggc
     7021 tgtgaacaag gattgtatgg ttggaatgtg acggactgta aaatctgttt taagtatggc
     7081 ttatactata gccctgttag taccccagca gattttcgga tgcttgctcc tattgtattg
     7141 gaacaagtct taaaaaaagc tggaacagaa ttgttagagc catatcttag ttttaaaatt
     7201 tatgcgccac aggaatatct ttcacgagca tacaacgatg ctcctaaata ttgtgcgaac
     7261 atcgtagaca ctcaattgaa aaataatgag gtcattctta gtggagaaat ccctgctcgg
     7321 tgtattcaag aatatcgtag tgatttaact ttctttacaa atggacgtag tgtttgttta
     7381 acagagttaa aagggtacca tgttactacc ggtgaacctg tttgccagcc ccgtcgtcca
     7441 aatagtcgga tagataaagt acgatatatg ttcaataaaa taacttagtg tattttatgt
     7501 tgttatataa atatggtttc ttgttaaata agatgaaata ttttttaata aagatttgaa
     7561 ttaaagtgta aaggaggaga tagttattat aaactacaag tggatattgt gtcctgtatg
     7621 tggaaataaa acacgattaa agataaggga agatactgaa ttaaaaaaat tccccctcta
     7681 ttgtccgaaa tgcagacaag aaaatttaat tgaaataaag cagttcaaag taactgtgat
     7741 tacagagcca gacgcaaaga cgcagagccg ataaaatgag attaatacaa tctcatttta
     7801 tcggctcttt ccgttatgta tggattcttt taattagtct tcgatgtttc ttgcttcgtt
     7861 gataccgctg gctaaagatt ccattaagga tagttctttg tctgtaaagc tatccatgta
     7921 tttctctatc tgtaatcgtc gggtgctttt taccaagtta ttagcaggta agaaaaattc
     7981 atcaacggaa acatgaagta acgatacaag gtcataaaga acttgtatgc tggggtgttg
     8041 ccctttattt tcaatattag ttaagtaccg tgggtcaatt tcaatcaatg ctcccacttg
     8101 ttcacgagtt aaacctcgtt tcaatcgagc ttctttaatg gctaaaccaa aggctctaaa
     8161 atcatattta tcttcttttt tacgcatagt agaccacctc tatacatttt attgttccta
     8221 ctgaattaaa aacaggtata gaaaaacgtg ttatatggtt tataggttta tatttaataa
     8281 aaagcactac taaacgccaa taaaaaaaac cgttatatgg tagtgctatt tacgctgtta
     8341 aaatattgta tattacttcc aaatggcggt ttgttggagg tcaacgtcgc catgaagtac
     8401 atcatataca ataaatttcc ttacattggg ttcttgtcaa aaaaagtcgt ctatctgcaa
     8461 tagataagta cgtccaccaa tgtggtttta taaatcatat agatagaata acagaagcat
     8521 gtaaacagag aaataaatct gtttatatgc ttttttggct attcagaact tttttacaaa
     8581 gtttatttat cagtaatgca acaaatcccc ctttcacatt gggactaaga gtgaaaggag
     8641 ataaacgagc aaggctcact tcctttccta gacagaaagg gggtgagaaa catgaaacca
     8701 tcttcttttc agaccacaat agaaaatcag tttgactata tctgtaaacg tgctatggaa
     8761 gacgagcgaa agaattatat gctttatctt tcaaggattg caaagcgtga ggtgtccttt
     8821 tcggatgttg gcgattatct tgttagccag tttgcgacaa cagataacta ttcaactgac
     8881 tttcagattt ttacactcaa tgggttatca gtaggcgttg aaaatgattt gttgagtgaa
     8941 gcattacgtg agttgccaga caagaaacgt gaaattctac tgctgtttta ctttatggac
     9001 atgagcgatt cagaaattgc agacctgttg aaattgaacc gttctactgt ctatcggcat
     9061 agaaccagtg gactagcctt aattaaaaag tttatggagg aatttgaaga atgaaaacac
     9121 aatatcctat gattcccttt cctctcattg taaaggcaac agatggcgat accgaagcga
     9181 ttaaccagat tctacatcat tacagagggt acataacgaa gcgttcccta cgacttatga
     9241 aagatgaata tggcaatcaa agtatggtcg ttgatgaagt cttacgtgga agaatggaaa
     9301 ccagactgat tacaaagatt ttgtcatttg aaattaagta atatcctctc tcctttcgtg
     9361 gaagcgtgct aaaccattcc acgcttcccg aacagggagg tttgttattc caccaaagca
     9421 tattgagctt tcaatgtgtt ttgataggct aacgagccat tgttctttga aaactgaata
     9481 aaagtaatcg aatacgtttc gataagaaaa gagccaacgg aactaaccgc catgacctat
     9541 cttataaaga tagcgagcga ttcatgttag tgatccgaga agcaatcttt agcaggattg
     9601 cctgcaacga cattcttatc gtgataatga tactcccata cagtcaatag tccgagcgtg
     9661 ataaaaccgt cgcaggcaat gagtatggct acatgagaac catgcagggg tggaactccc
     9721 gtgagctttg ctaaagctgt tcgattgctg gtaaaacaac ttttatgaaa tccaaataag
     9781 tgatttggaa aggaggattt tatgaagcag actgacattc ctatttggga acgttatacc
     9841 ctaaccattg aagaagcgtc aaaatatttt cgtattggcg aaaacaagct acgacgcttg
     9901 gcagaggaaa ataaaaatgc aaattggctg attatgaatg gcaatcgtat tcagattaaa
     9961 cgaaaacaat ttgaaaaaat tatagataca ttggacgcaa tctagcgtcg ccaaagggtc
    10021 ttgtatatga taaaatagta ttaagtcgta tcaaggctct ttccataaag gaaaggagca
    10081 aatgccatgt cagaaaaaag acgtgacaat aaaggtcgaa tcttaaagac tggagagagc
    10141 caacgaaaag acggaagata cttatacaaa tatatagatt catttggaga accgcaattt
    10201 gtttactcgt ggaaacttgt ggctacagac cgagtaccag caggaaagcg tgattgtatc
    10261 tcacttagag agaaaatcgc agagttacag aaagacattc atgatggtat tgatgttgta
    10321 ggaaagaaaa tgacactctg ccagctttac gcaaaacaga acgctcaaag accaaaggtt
    10381 agaaaaaaca ctgaaactgg acgcaaatat cttatggata ttttgaagaa agacaagtta
    10441 ggtgtaagaa gtattgacag tattaagcca tcagacgcta aagaatgggc tattagaatg
    10501 agtgaaaatg gttatgctta tcaaaccatc aataactaca aacgttcttt aaaggcttca
    10561 ttctatattg ctatacaaga tgattgtgtt cggaagaatc catttgactt tcaactgaaa
    10621 gcagttcttg atgatgatac tgtccctaag accgtactaa cagaagaaca ggaagaaaaa
    10681 ctgttagcct ttgcaaaagc tgataaaacc tacagcaaaa attatgatga aattctgata
    10741 ctcttaaaaa caggtcttcg tatttcagag tttggtggtt tgacacttcc agatttagat
    10801 tttgagaatc gtcttgtcaa tatagaccat cagctattga gagatactga aattgggtac
    10861 tacattgaaa caccaaagac caaaagtggc gaacgtcaag ttcctatggt tgaagaagcc
    10921 tatcaagcat ttaagcgagt gttagcgaat cgaaagaatg ataagcgtgt tgagattgat
    10981 ggatatagtg atttcctctt tcttaataga aagaactatc caaaagtggc aagtgattac
    11041 aacggcatga tgaaaggtct tgttaagaaa tacaataagt ataacgagga taaattgcca
    11101 cacatcactc cacatagttt gcgacataca ttctgtacca actatgcaaa tgcaggaatg
    11161 aatccaaagg cattacagta cattatggga catgctaata tagccatgac gctgaactat
    11221 tacgcacatg caacattcga ttctgcaatg gcagaaatga aacgcttgaa taaagagaag
    11281 caacaggagc gtcttgttgc ttagtagtac aaatgaattt actacttatt taccacttct
    11341 gacagctaag acatgaggaa atatgcaaag aaacgtgaag tatcttccta cagtaaaaat
    11401 actcgaaagc acatagaata aggctttacg agcatttaag aaaatataaa aagataatta
    11461 gaaatttata ctttgttttt acacaaaaaa atcacttttc ctaattacaa ttgaaatcat
    11521 tctaaaatga taatgttatt ttatattact atcttttttg tacaacaaaa actacaagga
    11581 ttctatattc tatatttttc cataaaaaat accccttatt atacaaataa attatttgca
    11641 taataagggg tatggctaag taagtgtttt aaaattttat gatataaatt aatattaata
    11701 atagacttaa attaataatt tatgtttaga tttatttttt aggtaagtgt aaacattctc
    11761 catttacatc tgccaaagct tccattaaag cttctgaagt acatggatga ccaaatatca
    11821 actcacttgc ttcatcagta ggtatctcta atgctttaaa tgatgctgca tgatttataa
    11881 gttctgctac accacatcca aacatatgta tacctaaaat ttctccatat ttagcatctg
    11941 ctactacttt cacatatcct tgttcttgac cagaagctaa agctcttcca tttccagcaa
    12001 aattaaactt accaactttt acattatatt tttttctagc atcctcttct gtaataccaa
    12061 ctgatgctac ttctggaatt gtataaacac aactaggtaa tgcacctaag tcaacttctt
    12121 tattcacacc tagagcattt gaagctgcta cctctcccat tttaaaagca gcatgagcta
    12181 acattacacc tccagtaaca tcacctactg catatatgct tggtatactt gtttccattt
    12241 tactatttac aactattgaa cctttatcta ttttaatatc taagtcctct atgccagaaa
    12301 gattagcttc tcttcctata gcatatagac ataaatctgc ttttattggc acttcacctt
    12361 caatgcacac taatatgtta tttccttctt ctttaaattc agaaactttc tttttagtta
    12421 atacatttat acctttttta ctaagagaat attttaaaga ttcgcttaac tctttatcca
    12481 ttcttgggat tactctatct tccatttcta ctatagttac tttagaacct cttgaattga
    12541 atatctctgc aaattcacaa ccaatcactc caccaccaat tattactaat tcttctggca
    12601 ctgtttccaa atctaatgct tctgtactag ttattattaa atttgattct attcctttta
    12661 taggtaatat ccttactttg gaacctgtag caattattat attttctgta tctaaaactt
    12721 ttccatctga taaaattact ttatgctctt ctttaacact agctttaaga ttaaacacat
    12781 ctacatctct actttttaat aatccgccaa caccagctgt aagtttttta actactctat
    12841 ttttatattt tatggccttc ttaatgtcct gttctttatc tacagttact tttacacctc
    12901 tctttgataa ttggtcaatt tcttctaata tttctgctgt ttttatataa gtctttgtag
    12961 ggatacaacc tctatttaag caagtacctc ctagtatatt ttcttcaact aaagcaactc
    13021 ttccacctaa aagagctgct tttaatgctg ataaatatcc cccaggtcct cctcctatta
    13081 ccactacatc ataatcatga ttacactctt ttttgtttat tttttcttct ttaacttcta
    13141 tattttgatt ctcttcaact atatcttctt ttgaaacatt tccagataaa tatttagctt
    13201 ttacttcctc ttgattttcc cctttttctg ctattacacc tattactgta aatactggta
    13261 atacttcacc ttcctcttta tgtatgatta cagcaagtgt accttctcct tctgactcta
    13321 tctccatatt aactttatct gttgttattt ctagtatagg ttctccaatc tttacttctt
    13381 cgccttcttg ctttagccaa gatactatag taccttcttc catggctact cctgcttttg
    13441 gcattattac ttctactgac ataggctctc ctcctattta ttttcatata ttttaattaa
    13501 gcaaatcata ttcacatata gttactgtta aaaataattc ttaatatata aaagctttaa
    13561 atttactgaa acaattataa taatataatc tattcaaact atttatatca gtaacgaaat
    13621 atcaaaacct atacaagcat tgataaagga ttttctaaca attcttttaa atcttttaag
    13681 aattttgcag ctactgttcc atctattact ctatggtctg atgttaatga taagttcatt
    13741 ataggtttta ttttagcctc tccatttact ggaacaaact tatcttgtgt agcacctacc
    13801 cctaagattg cacttgaagg catatttatt ataggtgtaa atgtagttat accatacatt
    13861 ccaacattac ttattgtaaa tgtattacct tcttggtctg ctggcataag tttccctgtc
    13921 tttacttttt ccgcaagttc tttgctttct tttgctattt cttttaaaga ctttttgttt
    13981 gcatttttta ctaccggaac atataagcct tcatctaaac caactgcaat agctatattt
    14041 acatctttat atctaaatat accctcatct gtccatgctg aatttaaggc ttgatgattt
    14101 ggaagtattt tagatactgc cattactatt aaatctgtca tagttaattt tactccagtt
    14161 gattctttta ctgtgtcgat taactttgct ctaagtacct tcaattctgt agcatctact
    14221 tctatattga atgtaaatac aggagcacta aaataacttt ctgacattcg ttttgctaca
    14281 gtagctctca ttggatttgg cttttcaaat attccttcta ctgtgttaaa catgcttgaa
    14341 ttttcatttt caattttcag ctcttttttc tcactatatt tagatgtatt atttgataca
    14401 atagtttctt ttgcagttaa tttttccaca tcacactttc ttatctttcc actatatcca
    14461 gtacctacaa tagtttctaa atctatattt aaatcttctg ctattcttct agctaaagga
    14521 gtagtatttg ctttattatc ttttaaataa tttaacacat ctactaaatg aatccttcca
    14581 ttttctccac ttccaatcag cctatctaat tttatattgt cttttcttgc ctgacttcta
    14641 gctgctggtg tggcttttat tttatttgcc accatatcta tccctcctta tctaacaatt
    14701 aaagatttta ttgcttcttc tatttcatca actcttggga caactgcttt ttctaactct
    14761 ggattatatg gtattggcac atcttttcca caaattcttt ttactggagc atctaaataa
    14821 tcaaatgatt cactttctgt tatcagggct gatatttctc cacctagccc accagtctta
    14881 gctgcttcat ggcaaattaa aactctccca gttttacata cactctttac tatagtctct
    14941 ttatctaatg gatataaagt tcttaaatct ataatttcaa catttatatt ttcttttgat
    15001 agagtctctg ctgcttcttc aacactttgt aacattcttc cataagtaat tactgtaaca
    15061 tcagtaccct ctcttttaat atctgccttt ccaatttcaa ttacataatc atcttctggc
    15121 acaaaacctt tttttctgta taacaattta ttctcaacaa atatgactgg attattatct
    15181 cttatagctg ctttcaacaa tcccttagca tctgctggtg tagaaggtgc aactactttt
    15241 actcctggta catgacaaaa ccaagcttct aaactttgtg aatgttgctc tgctgaacca
    15301 gttccagaac cacctggaca tctaacaacc attggaactt gtgcttttcc tccaaacata
    15361 tatctcattt ttgccgcttg atttacaatt gcatccatag aaattgttac aaagtccata
    15421 aacattactt ccataatagg tcttaaacca gttgctgctg cacctgctgc cgctcctgct
    15481 atagctgctt ctgatatagg agtatccctt actctttctg gtccaaattc atctatcata
    15541 ccaactgata caccaaatgc ccctccatat attcctatat cttctcccat aaatattaca
    15601 ttttcatctc ttctcatttc ttctgacatt gcttccttta ttgcttgtgc atatgttaat
    15661 tcccttgtac tcatttttat tttcctcctt taccattctt agtctgcata tacatcctct
    15721 aataaagact ctatttttgg atttgggcta ttttgagcaa actctactgc atcttctatt
    15781 gattgctttg caaattcttg aatcttatct aattcatctt cattagatag attattttct
    15841 ataagataat tttttaaaaa ttcaataggg tcttttgctt tccatgattc tatttcttct
    15901 tttgttctat atacatttgc atctgatttt gaatgaccta accatctata agttctactt
    15961 tcaattaata caggtccttc tcctcttcta cacttctcag cagctttttg tactgtttcg
    16021 tatacttcta ttgggttata tccatctatt gatattcctt ctattccata agaagctgct
    16081 ctagtggcaa tactttctat attcatatga cgttttatgc ttgttgacat accgtataaa
    16141 ttattctcac agtaaaatat tattggcaat ttccatattg aagataaatt tattccttca
    16201 tgaaaagtac cttcatttga tgctccatca ccaaaactac ataaaactat tttgcctgtt
    16261 tttttatact gttgtgtaag tgcagctcct ggagctattg taagacctcc acctacaact
    16321 ccatttgctc ctaagttacc tgactcaata tctgctatat gcattgagcc tccttttccc
    16381 ttacaaaatc cagtttcttt acccataagt tcagccatca ttttatttag gtctattccc
    16441 attcctataa attgactatg tcctctatga gttaatgata ctaaatctcc tttttctaaa
    16501 gccattacag ccgccacact tgacgcttct tgacctacag ataagtgagt tgttccatga
    16561 accatacctc ttgcaaaaaa ccaactaact ttctcttcaa attttctagc ctgattcatt
    16621 ctcttataca tttcaagcaa tgtttctttt gatattgaca tatacattcc ctcctgttaa
    16681 cttttatcta tctttctatt agtcacaatc ttctaataat ttaagtgcta aactgtgatt
    16741 aattgctaaa ctatttataa atttcgcttt taatgatgcc tttattgcct ctattttttc
    16801 atctcctgaa gcgacaccta ttgttctttt tatctttttt aaatcttcca gtttcactcc
    16861 taatactctt tgattaaatt caagtataga agtttttcct tctttatcaa aggcttgtaa
    16921 acaaattgca cctattgagt tgtatttttt taaatcttct atatctgctt cattcatgta
    16981 tccagaagcc attattgttg agtttaaact cataggattt cctataccca caattgctat
    17041 agttgtttta tctattaaat ctagtacttc tttaattttt tcgtctttgc aaagtttctc
    17101 ttttgtagac aagtcagaca taacagctgg agcatgtaat aatttaaaat cacctccaaa
    17161 agccctcgct agacttactg ctatttgatt tgcatgaata tctatttcat tgtctccaat
    17221 ccctcctaat agaggtataa atgtaacatt tttacagtta cttttttcca catatcttgg
    17281 tatttctttt agtgttgttc ctattgaaac acctattata tcattttcct ttactactct
    17341 agttaaatat tcagatacag ctcttgctag ttcttgtttt tgtgttagtg catcttgttt
    17401 atcatctact actattactt cttttaattt atattttctc tccaaacttc tctctatttt
    17461 ttggtaatca tttttcaaaa catcaactat ctgtattttt actattcctt gttcaaaagc
    17521 ttcttttaat attcttgata ttgttggtct tgatattccc aattgatttg ctatttcctg
    17581 ttgattcaaa ttatcttcgt aataaagtga acaacacttc atcattaatc ttaagtctcc
    17641 aatttttttc atttgatttc ctccaaaatc aagcttttca attatcttta catttgttca
    17701 atcattttac ttaatttcag taacattata gaaggggttc atataaaaag tcaactgttt
    17761 ttttgtataa caaaaaaaag gtgatacttt tcttgtacca ccaccttcat aaatagttga
    17821 aaaattgctt cttagcgcat attatatatt acttctaatt gttaaatatc atctccataa
    17881 attaaaaatt aattgtccac ttataatgct ttaaataaaa ataacttatt aaatcatttc
    17941 taaaagacca accatttcat catttaaagt cttcttatat ttttattatt cgcttctcta
    18001 aaaattcttc aataatcata aatatataat agtttttata gggaacattt acaatagacc
    18061 ttaattaaac ttaagacttt ataagaaact ttttcattat ttagtattta attcattaag
    18121 tatagtacta tattctttat caagcttata aaaaaacatt atgataattt gtaatattgt
    18181 tataaatata ggtaggtata taaaacatac ttttattgat actatagcta aatcactttg
    18241 agtagctgat gttcctacat aaccaccaat tgataatact gcacctaata ttgctgaacc
    18301 taatccattt cccactttaa acccaaaact agatgcacta tttataagcc cctcagttct
    18361 atatccagtc ttccattctc catattcaat agtatctgaa acaatagcaa acattgttga
    18421 tgctattcca gcacttccta ttccctttat agcacttgca aatattactc ctatatagtt
    18481 ttgtccaact attataaaca taacatttga cgatattaat attattgaac ctactattaa
    18541 tacatttctt tttccaaatt tttttataaa aggagcaacc ataaacatag ctataatttg
    18601 ggatatattt gaaaccatat ttataggacc aaataatttt tcatctccca aaataaactt
    18661 tgcataatat acggatgaac caccatttat cgcaaataca ataaatatgc aaactaaaca
    18721 taatgttatt tgtatccagt acttatttct aaataaagat ttaataccta ttttaaatgg
    18781 tattacttca ttcctatctt gcttaactgc ttttactctt tccttggttc cagtaaatgt
    18841 tataaaaaat accattatag atgctattcc aaatacaaag aatgttttag tccatgctga
    18901 taaattattt ccaaagtatt caactagagg tagtgtaagt ccatttatta gtaaggtacc
    18961 acaagtggct aatatcattc taaatatact taatactcct ctttcatact ggttttgagt
    19021 tataagtgaa tttagagttg catagggtac attgattgct gtataaataa cagtagaaac
    19081 taagttatat gtaataaaaa tataaattaa tttagaagtg ataccaagat ttgatggaac
    19141 agaaaataat aatactgctg ctatagcaaa aggaatcgcc attctcaaaa tccaaggcct
    19201 agccttaccg tatttggatt tagttctatc cactataatt cccattgtta agtcactaaa
    19261 cccatccagt atcctagata caagcataat actacctaca gctgctgcac taacatctgc
    19321 ataattagta taataaaaca gcaaaaatgc tgacatagct gagtatatga tattgcaccc
    19381 caaatcccca caaccataag aaattctttc aaaaaatgaa atcttttcat atctaatcgt
    19441 agttgcttta acattaactt ctgtattcat aataccatac tcctttttgt aagatttgcc
    19501 ttctaatgtt tcttatttca gagcataaga tttacctttg ctctgaaaat tttagtaatg
    19561 attttattaa tcgacatctt ttgattcatc tgctatatat tcaaaaaaat caaaatcagc
    19621 atagtgttta tgctttacac gatctgcaca agttaatcct ataaacgtac ctgtaaattc
    19681 tccatattta cagtactcat ctgaaaactt agttgtatca aatacctttc ctattctttg
    19741 gtagtttttt tcatcataac tccattcaaa atatgactta cgaccttgta tatacagacg
    19801 aagatatatt ggtatatctt ttattggtat cctagtattt aaaaattccg tcttctctcc
    19861 attttcaaga tgtattattg atagtgcact ttgacccaat gtttcactat aatactttct
    19921 taaatttata taattcatat tatcataata cattatcaaa cccgcactat gctgatgtac
    19981 ctctgggtaa aactccatct tagtggttat tcttgcatat actgaagtta attttcttgc
    20041 caatatgctt actttattta atgatgttct tgattcttga cctctaatcc ttacatatcc
    20101 tggtctagac ttaacatctg caaatgataa tggtgaaatc ctaggagcat agtactgtaa
    20161 acctaataca tttgaatcaa agtcatcaaa acttggtata gatgaaacta aatgttcttc
    20221 tagtttactt tctgatacgt attcttttgc caaattactt tcatcttcca ttcgaagcca
    20281 attatccttt gtccatttca ttttttgaat agctgtttct ctacctaaag tacaccttaa
    20341 ctctggtaca aatggtctag atgttagatg aactaaatat acttcacctg atgttgtttc
    20401 tatatagctt ccatgaccag atttttgaag ttttgattct ggattatagt atttaggctt
    20461 tagatggtca gggtcatgtc tttcataaaa atcgcctgga attgaagtta caattggatt
    20521 cattgaatct ttttcataag gtctccatat atttttagcc cttcccatag ttacactatg
    20581 accatatcca gttccacctt ctgcacacat tatataatag taatctccac gctttgttat
    20641 atgtggtgct tcaatacaac ctctatcagt tccccctgac catattctct ttggatatcc
    20701 tactatttct ttcttttttg tacagtattc cactaaacat ataacaccag gtttttcata
    20761 tccttctcta gtctcccagt ctaatgatgc aatccacttt ttaccatcat catcatgaag
    20821 aatagacgca tcaaaacctg atgagtgtat atatactggt tcactccact ctccctttat
    20881 atcttttgat gtaattaaat aattatcaac atcaaaatat cttgcattca tagagttcat
    20941 aattccataa actatgtaaa ataaatcttc ttcttcacaa taagtcaaac aaggtgccca
    21001 aattccttta gatgaaggaa gcttcttcaa atctattttt gtttcatctg tcaaaatatg
    21061 agtatataac tcccaatttt ttaagtcttt agagtgataa acaggtatac ctggaaacca
    21121 ttcaaaagat gaaactacaa gataataatc atctcccttt ctacaaatac aaggatctgg
    21181 attaaaacct ggaagtatag ggtttttaat catatctttt cctccaattt tagtttaata
    21241 ttctatagga taccattctt gagctacaca tttgcctagg tcccaactat cccatcctat
    21301 ccaaccttct tcattaactg tatctagtaa taacttataa ttccaatagt agtgaccact
    21361 tccatttctc catgcatcaa gctgtgcttt tgctatcttc ctatataatt ctcttttatc
    21421 gtcttttgat aatttatcta tatttgattc aatcccattt agaggtgatt gacctccatt
    21481 tgtatctata ccacaagcat atgaattaaa taagctccac tccccacata taacaggaaa
    21541 atatttttgc atttgtaaaa tatctttagc ataattttct ctaatatatt tgagatagct
    21601 atcaatactt tgttcacatc catctgattc agctaacatc aaatattggt gtgtatctag
    21661 aacaatattt ttaaattctt cctctctcat aaagtctttc catgctttta attcaaatgc
    21721 atcatgaaac actataacgt tttcctctct catataagga cgcattacct tatatgcttt
    21781 tatataaaaa tcacgtaaaa actctaatga attaggttta ctcccccttg ccatctcctt
    21841 atctacagcc ttaaaccgat ttggaacatt cattatgttc cacattcttt ctgttatagg
    21901 ctcatttaaa atttggattc catacaattc atgtctcatc ccataacgtt ttgcaagtct
    21961 ttctaataca tttaatgtaa acgctacata ttctggattt tgtgaccagc tacatatacc
    22021 tgatattcct ccattatcaa atccattttg gcttcctgga actgtatgta agtctatcaa
    22081 tatactaagc ccatacttat ctgcccaagc aaatgcttta tcaagttctt taacacatcc
    22141 tatgaatggt tcacaatctc caaaaataaa gtatggtact ggtatacgaa ctgaattaaa
    22201 tcccatagat ttaatcgttg caaaatctct ctctgtgatg tattcactac gatgagtctt
    22261 aattctagat tcatatgcct ctcttgaaag ctgtcttggt aaatagtatt catcttcagc
    22321 ttctgtgcct tcaaataaac ttgaggtcat ccatttttct aaaactaacc aacctcctaa
    22381 atttacacct tttatataca cataaaatcc tcccctctta tcttatttaa ataggtatat
    22441 tttgcactta taataaaata atttttttaa gttattttaa tatacagtta ttatatttga
    22501 ttttatccgt tttcttaagt atgtttatta tctaatttat tagtctatat tatcttttta
    22561 atattgaaca ttataaattt actatttata atttaaaatt ataaggagga tattattatg
    22621 aacagaaata atttttcaca tgaaataatt gaacctattg ataatttaga tgttaatttc
    22681 aaactatttg atgatagtgg tagttatgta gcaaatcatt ggcataactc attagaaata
    22741 atttatataa cttctggtga tttacaaatt aacatggagg gttacactta taatttaaaa
    22801 gctaatgagt gtatgttcat taattctgga attattcact caactagatg tacttatcaa
    22861 gatacctcta tattattgca agtaccattg tcttttctaa acaaatatat ccctgatttt
    22921 aaaaattgct attttgactt taaggttaat gccaatgaca ataactataa aaaaaattca
    22981 ggtaaagtta aacttatatt agaaaacatg agagaaatta aacttagctc tcctcctgca
    23041 gctaatctgc tctttacaag cttattattt gaacttttat ttgaattata tacaaacttt
    23101 aaaatatctg taggtaataa aaatatgaaa aaaacagtac ttgatttgtc aaaatttgaa
    23161 ccagttttag aatatacaaa gattaattac aaaatgccta tttctattaa caaaatcgct
    23221 aaggtagcac acttacagcc agaatatttt tgtagaaaat ttaaacagta tatgggacaa
    23281 acttacttag agtatttaaa tgatgtgcgt atttctcata tttataaaga cttaattaac
    23341 acaaatgata ccttatgcag tatattagaa gctcatggat ttactaatac aaaattattt
    23401 tacagaattt ttaaggaaaa atttaaatgc actcctaaac atataagaaa aaaatctacc
    23461 taaaatatga ggttatatgt tatttatctt tataataaaa aaagtttacc ttaaaatttg
    23521 attagaataa tcaattttga ggtaaactct tttttattat aatttatttt ttataaactt
    23581 tcttctttat tttgtaaata aagaagttaa attaatgtgt tcatatttac atattattca
    23641 tacagtactt attcttttaa tgctgtgaga tttatactta atgttatcat aggtacaatt
    23701 acataaataa aggctgtagc tgatgacaca atcccagagt cattcactaa aagagttact
    23761 aaacatcctg ctattgatgc tataaatcct ttaaatacaa ttggatactc atccatcagt
    23821 tgcttaaagt atctaatctg attaatcata agaactaaaa ttacacctat tccagttaac
    23881 agtatattta cccatgcact ggtttgagct agctttaaat tcatctctat ctttctacta
    23941 aatgtttgta ttatttctcc tggtccatta aagtaaattt cttttacaaa catacctaaa
    24001 tgtgaattac ttcccaaaat catatctatt gctacaaaaa ctcctaatac aaataatact
    24061 gctataccta ataatacaat ttttttgaag tctatcttca cattatatat caatagtata
    24121 aatagtaaaa atgcaacaca ttcagaaata gcacttccaa catttgctcc cataattgga
    24181 gatgcacttg taattaagat aaccaaagaa aaaactatta tagaccactt tggtatattt
    24241 ttatatgtaa gtaaaattgc aaatgtaaat attgcactac cgattgccac accttgatac
    24301 tcatttccca ctccataata tctagctcca attatacagt cataactcat aacattgctt
    24361 ttcattaaat atgagccaaa cccagcatca ataaccataa ttactatggt taatccagta
    24421 aaaaacagca tgttttttaa atcattcttt attaaaactt tacttataag atatatagta
    24481 agtgttatta taattattcc taaagatatt gctaatggtg ttttgaaatt cattattggt
    24541 gttatcataa atgacagtgg cattactatt cctattttta aaagttcctt taatataaaa
    24601 aatgttgttt ctttataatt ttttgatata tgctttctaa ataaaattgc aatcatagca
    24661 actatccatg acaagaaaac tatacttaca aaaccattca gtattgtaga ccttatgctt
    24721 gaaatagata ctatcttttg atattcatcc atcaaaaatt ctttattatc atctctgtta
    24781 ataagttcat attttcttcc aaccatattt tgatttttta tattaaagtt gtctaatatt
    24841 tcaaccccaa catctaaatt tgttacaata ccttctcttc tagtagttga agatgataat
    24901 atacctttcc cttctccatt taattttatt attggagaaa gtcttctttt atttttataa
    24961 tctaaatcac ttggaaatgc acttgctatg tagacagtgt cattttcttc aaccatagaa
    25021 aatatatttt ttaaatatac atctatattt ttttctatgt tatctttcac actctcatat
    25081 gttttttcat ttaaatttgg tttatataaa tctaatctat atgtgtctcc aagctccaca
    25141 aaaaccacat cattattttt gtaagcttct ttggtctcta caatcaattt atcataatca
    25201 gttgatattc cataaggcat agataagtct tttttattta tagtatctac atttccatta
    25261 ggtattcttc catattcatc cattgcagtt agacataagt ttctattttt tataagttga
    25321 ccattttcaa ttatatctga attacccaat acagatgctt ttaatccatt ctcagataaa
    25381 ctttgtccta atgaccctaa tacagaacca tactctccaa agtttaaatt ttcatttata
    25441 gacttattta tagttaaatt atttatacct tttgcacttt ttcctgtagc tgattcaaaa
    25501 actatatttc tatccttact cgagctttca aaattaatat cttcttcatt agccacatta
    25561 gctctaccac cagcacccat actagcatat gaccttctat catcacttcc tttatcgcct
    25621 cttatattca tcaagccaat ataccctcta ttgtctagtt cttctcttaa tgattttatt
    25681 cttaacatat tactcatact agttctattc atatctataa atattacttt tccacgtgtt
    25741 gacatatcat ctgcaaaact atactgactt gctgatgtta atattatgat aaatgttgct
    25801 aagaaagata taatcttttt tctcataaat tctccctgct ttctgattta aatccccatc
    25861 tttaagaata cattaaacat tatcgttata aataaataaa tttggaccat ctatttaaaa
    25921 taaacagccc aaattatagg tttctttgaa catattaatt tttaggtttt gcaaatatca
    25981 ttctacctgc aggagtttga agaacagaag taacgagtac ttttatagtc tcattcatat
    26041 attttcttcc tccatcaaca actatcatag ttccatcatc tagatatgca acaccttgtt
    26101 gagcctcttt tccttcttta accacttgaa caaccatatc ttcacctggt atagcaactg
    26161 gttttatagc attagctaac tcatttatat ttaatacttc aacaccttgg aattgagcaa
    26221 ctttatttaa gttataatca tttgtaacaa cttttccatt taaaacctga gcaagcttta
    26281 ataatttact atcaacttct gctatatcat caaagtctct ctcacttatt tcaacttcta
    26341 tatttaattc tttctgaatt atatttaata tatccagacc tcttctacct cttactcttt
    26401 ttaaatcatc tgaagaatct gcaatatgtc ttagttcctc caaaacaaaa gcaggaataa
    26461 taagttttcc ttctataaac ccagtcttac aaatatcagc tattcttcca tcaattatta
    26521 cacttgtatc taatactttt ggaggaatgg cttttacttc ttttttattt tctttatcct
    26581 tatctttatc cttaatagga tttgctaatc ttcctagttt tccaatatta aataaatcat
    26641 ttttactttt aagcgcaact ttcattccaa gatatcctag gaataaatat aatagtaagg
    26701 atagtattcc acctactatt ggtattctat ttactaatcc acttaataaa tacgcaataa
    26761 caaatcctac tataagaccc atagacccta acaatatatc agtctgagga tattttgaca
    26821 attctttatc cactatttta gcaatttctt ttactttatt tacaacccat ggttctataa
    26881 gatatcctaa aatggctatt attatacctg ctactacagc tgcgatataa ccatatgttt
    26941 cttttccaaa tgttaatatt tcaaaatctt tcattaatgt taaataggtt gttataccta
    27001 ttgtaaatcc aagtaaaata aacaatattc ttgttacttt ccttatcaat gcttcacccc
    27061 ctagttatac ttatctatta tagaccaaag tgtatattat attcaagaaa aaagtagtaa
    27121 aattcattta aaattcatca tatgtgtcta tctaataaga ctaattgttt catttttatg
    27181 agtccatttt ttatatatgt tgctcttatt tcacctattc cttcaacctc atctaattct
    27241 tcaatagatg catctaaaat ttgttgaaag ttttcaaaat aatttactaa gttttctatt
    27301 attgctgttg gtagtctatg tattttgcta agaatcctat aacctcttgt ttttataggc
    27361 atatccatac tttctgaaaa accactatac cctaatagtt ttgccatatt gaccaattct
    27421 attagttctt ctgaatttaa atttttgacc tttttcataa gttcttttat ttctgtgttt
    27481 tctttattat aatctttgaa tattaatttc tggtctattc tggttgtacc cattaattct
    27541 tctaattgca tacttactaa agttccttca tcacccaatt ctatcacata tttttcaatt
    27601 atacttgtaa ctctcattac catttccatc ttttgcatga ctaatgcaac atcataaata
    27661 gttaccaaat cattaaactc taaggcattt aaatttgtta cagcttggtc caatactgtc
    27721 ttatattttt ccagagtttg tatagcctga tttgccttag taaatatctt agatatatct
    27781 tcgactacat acttctcatt tcctctataa actgttataa catttcttct ttgtgaaatt
    27841 cctatgacta tagcaccagt ttgttttgct actctttctg ctgttctatg tcttgttcct
    27901 gtctctgatg tttctataaa atagtcaggt ataagttgtg cattagcaaa taatattttc
    27961 tttacatcac cacttaaaac tatagctcca tccattttag ctaattcata tagatatgat
    28021 ggtgaatatt ctgcatttat agcaaatcct ccatctacta ttttcattac atcttcgttt
    28081 gttgcaatta caattaatcc accagtctta gctcttagta cattgtttag acctaatcta
    28141 agtggagttc caggagatat catttttaat gcatatagca tatttttatt atctagaaaa
    28201 ttctccatat ttattgattc ctcccaagta ctatatttat agcttgtctt aagttatcta
    28261 ctggccatat ttctatgcct ttggtctctt ttacaacatc ataattactt ctaggaacta
    28321 ctatcttttt aaatccaagt tttttacact cagctattct cttttctata aagcttactg
    28381 ctctaacttc tcctgtaagt cccacttctc ctgtaacagc tatattttca tctatcggta
    28441 tatttctaaa actagatgct acagaaatgg ctatccctaa atcaattgag ggttcattta
    28501 tctttattcc tccaactatg tttatataga catcttgatt ttgtatttgc aaaccaacac
    28561 gtttttctaa tactgctaat aacataccta ctctattgta atcgactcct gtagatgttc
    28621 tttttggtat accaaaactt gtaggagaaa ctaatgcttg caattcaagt aacattggtc
    28681 tcgtaccttc tacagtagat attattactg aacctgctac atcttttggt ttttcagaga
    28741 ttaaaatttt agatggatta tcaagttcta ctaatcctaa atccctcatt tcaaatactc
    28801 caagttcatt agttgaacca aatcgatttt taacagctct tactaatcta taagtattat
    28861 atctttctcc ttcaaaatat agaacagtat caaccatatg ctctaataat tttggtcctg
    28921 ctagtgaacc ttcttttgta acatgcccaa ctatgaacgt tgaaattccc atttttttag
    28981 aaattttcat gaattttgaa gtaccttctt taatttggct gacagttcct ggtgccgagg
    29041 atatttctgg actgaagaca gtttgaattg agtctagaat tattaattct ggatttacac
    29101 tttctaaata agattcaatt atacttaaat tattttcagc aaatatatat aaattttctg
    29161 agttaatacc taatctttta gctctcattt ttatttgaga ttctgattct tctccagtta
    29221 tatataaaac agtttttcct aaattggcaa cattactaga tacttggatt aaaagtgttg
    29281 atttacctat accagggtca ccaccaacaa gcactaaaga gcctttaact atacctcctc
    29341 caagcactct atctaattca tttatatctg ttgtaaacct ctcttcttct ttactttcta
    29401 ttgagtttat actgactggt ttagatgaag atttatctat tataaagacc tcttttttgg
    29461 tgcttttttg gtctatttct tctacaaatg tattccattt tgtacattct ggacatttac
    29521 caagccattt agctgtttca tatccacaag attgacatac atattttgtt tttatttttg
    29581 ccatactttt atcatcctta attttttatt ctatactata attttagcat atctgaaaga
    29641 caatatgtaa agattaaata aaataattaa ggacaatgaa ttattataga ttcattgccc
    29701 ttaaaatttt gtttagatta taaaacttat atttgcaata ttttaaaatg caactctatt
    29761 aaattattta gtttcaaaaa ctattttttc atctttgact tttgctacta cattacttcc
    29821 ctttttcaca ttacctttta agattgcttc agatagctca tcttccaact ctttttgtaa
    29881 agctctttta agaggtcttg ctccatattc taagtcaaat ccagatttag aaattaattt
    29941 aacagcctca tcactcattt ctaattttat atccatttct tttaatctct cttgtagttt
    30001 cgctgccatt aaaagtacta tttttgatat atgattttca tttagtgagt ggaaaacaat
    30061 tatatcatct attctgttta aaaactctgg tctgaatctt tgttttaatt cacccattat
    30121 attttctttc attttttcat attgagattt ttcttcttcg tctcctttag ctatactaaa
    30181 tcctaaagtt ttttgtctac caattgtaga tgctccaaca tttgatgtca ttattacaat
    30241 agtattctta aaatctacag ttcttccttt tgaatcagtt agtctaccat catctagaat
    30301 ttgtaacaga atattaaata catctggatg agctttttct atctcatcaa ataagataac
    30361 tgaatatgga tttcttctta ctttttcagt taattgacca ccttcatcat gacctacata
    30421 acctggaggt gaacctatca ttcttgacac agcatgtttt tccatatatt cagacatatc
    30481 tattctaatt atttgatttt catctccaaa ttgcacttct gctaatgcct tagataattc
    30541 tgttttacct actcctgttg gacctaaaaa taaaaatgaa ccaattggtc tattagggtc
    30601 tttaagacct gctcttgacc ttctgattgc ctttgaaata gattttactg cttgttcttg
    30661 acctataact ctattgtgca gtatttcctc aagtctcaaa agtctatcag cctcttcttc
    30721 aagaatttta ttaactggta ttcctgtcca taagcccact acttcagcaa taacttctcc
    30781 atcaactaaa tctgaatgtt tagatgactt gttccatctt tctctaacat cttctaattg
    30841 ttttttaagt agaccttgtt catctcttat tttggctgct ttctcaaagt cttgacatct
    30901 tactgcttct tctttttctt tatctatatt ttctatttct agttctaatt tttttatttc
    30961 tgaaggaggt gtattttctt ttaatcttac ttttgatgca gcttcatcaa tcaagtctat
    31021 agctttatct ggcagatatc tgtctgatat atatctagtt gataattcta cagctgcctt
    31081 tatagcatca tcagttattt taactttatg atgagcttca tatttatctc ttaatccttc
    31141 taatattttt attgagtcct ctttagttgg ttcatctacc ataactggtt ggaatcttct
    31201 ttctagagca gaatcttttt caacatgctt tctatattca tcaattgtag ttgcacctat
    31261 aacttgtatt tctcctcttg ccaaggctgg ctttaatata tttgaagcat ctatagagcc
    31321 ttctccagta gacccagccc ctattattgt gtgcatctca tctataaata agattatgtt
    31381 tccattttta acaacttcat ctacaacttc tttaattctt tcttcaaatt ctcctctata
    31441 cttagcccca gccaataatg aacccatctc taaagagtat aaagttttgt tttttagtgt
    31501 ttctggaaca tttcctaagg ctatatttgt agccagacct tcagctatgg ctgttttccc
    31561 tacacctggg tctcctatca acactggatt gtttttagtt cttctactta atatttgtat
    31621 tactctttgt atttcttttt ctctacctat tacagggtct attttattct gttttgcata
    31681 tagtgtaaga tttcttccat acttatcaag tactttagat tctgcttgat tctggttgcc
    31741 cgtgtaacta ttctctgctt tgtattgatt tttatcacta atacccatca tatcaattgt
    31801 taattgggct aaagtcctat catttactcc agcataattt aaaatcttat tagctattcc
    31861 ttcaccttct tgaataattg ctaataatat atgctcagtc ccaatataat ttgtttttaa
    31921 tttatttgca aacattcctg ataattcaag tatttgttta ctccttggac ttaatactat
    31981 atcttctgag atttcttctc ccttaccttc catatcaact attttgcctt ctaaataagc
    32041 ttctgtaaat ccaactttac ttagaacttt agcagctata ccttcttctt ctcttaaaag
    32101 acctaaaagt atatgttcac ttccaacaat attgtgtcct agacttttag cagattcaaa
    32161 cgctaaatca attgcctttt tagctctttg tgtaaatcta ttgaagttca caattcatcc
    32221 ctcctcttaa tttttccaaa gtttctctaa tatatgttgc tctatttata tctctattct
    32281 caacatcatc actcttatac attattgact gatgcgctgg ttgtatacct atcataagtt
    32341 gttctatagc ccttaaatct agtttatcta tatagttcat ttctattccc atttttatat
    32401 ttgatagatg gctcattgcc tctgcgctac tcattactct tgaattttca agtgtcccta
    32461 tcgatctaaa tattttatct tctagtttta tacctaattt tttttgcaat atttctctag
    32521 cttttatttc ttttgaaatt gcatcttttg taagtccact tacattttct ataatattag
    32581 actctgttct tccaagtgta agttgattag atatttgata tatatttccc aatgcttctg
    32641 ttcgttctcc atatatccct ctaattgcta tacctatttg tgatgaaatt ttataaagtt
    32701 catccatata gcctagttga cttaatgctg gtaaatgcat cattacagaa gctctcattc
    32761 ctgttcctgt attagttgga caagatgtta gataacctag ctttgtgtta aaggcatatt
    32821 ctaaacttga ttctaacaaa tcatctattt catttgctac actatatgca tattctaagt
    32881 ttaaatcatc acatatagtt tgtatcctta tatggtcttc ttcatttatc attatgctta
    32941 tagttttatc tttttttact ataactgcac ttttatcatt ttctgctaaa tctgggctta
    33001 ttatatgttc ttcaaccata agcatttttt tagattggtc taaatcttca attttataga
    33061 aatcaaattc ttctttttgt tctaagttag aattaataaa cgcatttttt actttttcta
    33121 ttatttccat ggcacattct ttatctaatt tatttggaaa tggatagtta tttaaatttc
    33181 tagctagcct aactcttgat ttcatgacta tgttctcttt cataatatca ctccaatctt
    33241 agtcaataga accttttaaa tatttaattt tatctctaaa ttgagctgct aattcatact
    33301 cttcattttt tatagctcta tctaaatctt cttttagttc tttaatctca ttttctacac
    33361 ttatcttata aaaagatttt ttaggtgctt ttcctgtatg ttctatatgc ccatgtatat
    33421 tttgaagcat tggatttact tgactactaa atgcattata acattcacta catccaaatc
    33481 tcccattatt cttaaactca ctatatgttg tattgcattt aggacaaact agcttttcct
    33541 caagttcttt tttaggttga aaacctaaat tagaaaatat atccatcatt gaaaaaggca
    33601 tatctaagtt aaaattcatt tctgtattct cttttgcaca ctcactacat aaatacatct
    33661 ctgttttttc tccattgact atcttattat aatatacaga tgctttattc ttattgcatt
    33721 tttgacatag cataaattat cactcctcta tactaaataa tcctattatt atattcttta
    33781 aaatattaga ccttaccttc tcttttaatt catatattgg catacacaat gatttatcat
    33841 ctattgcata taatattaat tttaattctc tttcgttaat caaatccgac tcttttaaac
    33901 cttctaataa ttcctttgct tttttatagg atatctgact tcctattttt tcatttagta
    33961 gttctctaat atgtccatct ttactttttc gtattttttc tatttgaaca tatcctccgc
    34021 cacctttttt actttcaatg taatatcctc tatctattgt aaatcttgtt gttaagacat
    34081 aatttatttg tgatggtgca caactaaaca agtttgctag ttcatttctt tgaatttgta
    34141 tgctattatc ttcttccatc aaatctttaa taaatttttc aattatatct gtcatagttg
    34201 ccattaatat caccacacac ctttttattt ttgactttct ttgactatta ttttacacct
    34261 attatttaaa tttttaaagc cctaaattgt ataaatttaa attttttata gaaatttaga
    34321 tatgaattta ttctttcatt aattatgagt ttttatttat tatatttatt gataaccaaa
    34381 tgctaatttt ttgcttcatg attagatttc agaactttaa atactttata ttttaaatat
    34441 accttctcat ctttattact aaac
//
LOCUS       DAMJVX010000405        20713 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.guided.061, whole genome shotgun sequence.
ACCESSION   DAMJVX010000405 DAMJVX010000000
VERSION     DAMJVX010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20713
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.guided.061"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            145..1743
                     /locus_tag="PC407_003844"
     CDS             145..1743
                     /locus_tag="PC407_003844"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HDF2499207.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFDVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1978..2169)
                     /locus_tag="PC407_003845"
     CDS             complement(1978..2169)
                     /locus_tag="PC407_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HDF2499208.1"
                     /translation="MAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQ
                     SAGTAATLTIFRIADTVMT"
     gene            complement(2338..2931)
                     /locus_tag="PC407_003846"
     CDS             complement(2338..2931)
                     /locus_tag="PC407_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HDF2499209.1"
                     /translation="MRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYY
                     NLRTITWNDRVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNY
                     PYQIFEFTSSRDCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSIGPMG
                     ATGPTGATGNSSQPIANFLVNAPSPQTLNNGNAITGW"
     gene            complement(2932..3078)
                     /locus_tag="PC407_003847"
     CDS             complement(2932..3078)
                     /locus_tag="PC407_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HDF2499210.1"
                     /translation="MRKIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFF"
     gene            complement(3756..4280)
                     /locus_tag="PC407_003848"
     CDS             complement(3756..4280)
                     /locus_tag="PC407_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2499211.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(4345..5097)
                     /locus_tag="PC407_003849"
     CDS             complement(4345..5097)
                     /locus_tag="PC407_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2499212.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDISKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(5128..6426)
                     /locus_tag="PC407_003850"
     CDS             complement(5128..6426)
                     /locus_tag="PC407_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF2499213.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQQGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREVNVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(6443..8707)
                     /locus_tag="PC407_003851"
     CDS             complement(6443..8707)
                     /locus_tag="PC407_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF2499214.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(8962..9780)
                     /locus_tag="PC407_003852"
     CDS             complement(8962..9780)
                     /locus_tag="PC407_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2499215.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGIDEKIIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIIL
                     LDEPTAGLDPVSTRSIVDIIKGLNKDNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFVNENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(9773..10471)
                     /gene="cbiQ"
                     /locus_tag="PC407_003853"
     CDS             complement(9773..10471)
                     /gene="cbiQ"
                     /locus_tag="PC407_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF2499216.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIKNIFVLSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEIFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKMHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(10474..10758)
                     /locus_tag="PC407_003854"
     CDS             complement(10474..10758)
                     /locus_tag="PC407_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2499217.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPDYEPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGVQKGKRS"
     gene            complement(10748..11500)
                     /locus_tag="PC407_003855"
     CDS             complement(10748..11500)
                     /locus_tag="PC407_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF2499218.1"
                     /translation="MKQNIKLGVIAALMLIVLTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLVGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIISFLIFKALKKKDG
                     NNAMPVFLAAAIGDLATYTVTSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(11689..11881)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(12013..13785)
                     /locus_tag="PC407_003856"
     CDS             complement(12013..13785)
                     /locus_tag="PC407_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF2499219.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYNMDKHPALDSMTRRIVNDIRNIDTN
                     QDMMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(13799..14728)
                     /locus_tag="PC407_003857"
     CDS             complement(13799..14728)
                     /locus_tag="PC407_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2499220.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            14957..15145
                     /locus_tag="PC407_003858"
     CDS             14957..15145
                     /locus_tag="PC407_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2499221.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRAKRRKEVQYE"
     gene            15138..15839
                     /locus_tag="PC407_003859"
     CDS             15138..15839
                     /locus_tag="PC407_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2499222.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLLDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSINNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            15841..16764
                     /locus_tag="PC407_003860"
     CDS             15841..16764
                     /locus_tag="PC407_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2499223.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            16921..17844
                     /locus_tag="PC407_003861"
     CDS             16921..17844
                     /locus_tag="PC407_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2499224.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGCEKRVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDHTLRLYNTTLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            17837..18574
                     /locus_tag="PC407_003862"
     CDS             17837..18574
                     /locus_tag="PC407_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2499225.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFASIALGRRK"
     gene            18585..19358
                     /locus_tag="PC407_003863"
     CDS             18585..19358
                     /locus_tag="PC407_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2499226.1"
                     /translation="MWKLLWAECQKIRRSKIVWITFFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            19374..19526
                     /locus_tag="PC407_003864"
     CDS             19374..19526
                     /locus_tag="PC407_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499227.1"
                     /translation="MKKIKKLIILIVFLFLFGMLHCYTEWKFNNDPPKGVIERINEWT
                     DKIGLD"
     gene            complement(19899..20507)
                     /locus_tag="PC407_003865"
     CDS             complement(19899..20507)
                     /locus_tag="PC407_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF2499228.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         6822 a         3471 c         2626 g         7794 t
ORIGIN      
        1 aatttgtagt atttatgtaa gaaataaatt ttatataata ttttttcact aaattttttt
       61 ctttttacaa caaatactca ctaccttttg atataatatt tttatcaatt attattatat
      121 tcaagttttg aaaggagtat taaaatgagt ttattagttt tcggacataa aaatccagat
      181 acagattcaa tatgttctgc aatttcatta gcacatttaa agaacaaact aggaataaaa
      241 gcaacagctt atgctttagg agatatcaga aaagaagcta aatatgcctt ggattacttt
      301 aaggtagatg cacctgaaat tttagataat gttagaatac aagttagaga tttaaattat
      361 gataaagtag tacctttaac accaacttca tctatattag aggcttataa ccttatggat
      421 gaaaaaaatg ttaaaacact acctgtaact tatgatgatg gaacttttgc tggtatcatt
      481 actatgaaag aaatagctaa aaatctactt catcaacact ttacaactat acacacatca
      541 ctatcaaaca tttgtaaaaa ccttaatggt actattcttg tggactgtgg tgaagatata
      601 aaaggtagag tagtcacttt atcatttgga atggagactc taatagagac tttaaaagaa
      661 ggagacatag ctattgttgg agatagatat gatagtattg aatatgctat tgatacaaaa
      721 gttaaacttt taatacttac taatcataca gagatttcaa aagatttgct agctcttgct
      781 aagataaatg gagtgtctgt tgtttctgtt gaaagtgaca attataaaac ttctaatgta
      841 ataaatcaat gtagtttctt agactctata gaagctacag aaaatttaat tacattcaaa
      901 gaagatgatt atatggatga aataaaagag attatgctag aaacaaactt cagatcttat
      961 ccaatattag atgatgataa taagttttta ggtttagttt caaaaggtca tctattaaac
     1021 ccatctaaaa agaatgttgt tttagttgac cataacgaat atgctcaaag tgctgatgga
     1081 attgaacaag caaacatagt tgaaataatt gaccatcata aacttggtgg tatttctact
     1141 gatgttccta tgtcatttag agttatgcct gttggatgca atagtactat aatttatcaa
     1201 atgtacaaag aaaacaatgt tgagatacct tatgaaatag ctgggcttct tttatctgct
     1261 atattatcag atacattatt atttaagtct ccaacaacaa ctgatatgga taagaaagct
     1321 tgtgaagaat taagtaaaat agcaaaagta gacatggaaa aatacgctat ggatatgttt
     1381 aaatgcggta cttctttaga tgaatatact atagaagaaa tagtaaatat ggattttaaa
     1441 gaatttaata tgagtggaca tagagttggt attggacaag tatttacctt agacatagac
     1501 tctatatttg ctaaaaaaga tgagttttta tcttatataa attcgactga ttatgacaaa
     1561 ctaattcttg cggttacaga tataatcaaa gaaggttctt atttaatcta taaagcagaa
     1621 gatagcttaa ttgctaacgc atttgatgtt gaaggtgttc aaggtacatt tgcaccaggt
     1681 gttgtatcaa gaaaaaaaca attagtccct aatttaacta ctgcaatcaa aaattttaaa
     1741 taatataaaa tttttaaata acgaaaactg tctcaaatct atatttgaga cagtttttta
     1801 ttatacatat attgataata aagttaaata aaaatccata aatttaaaat ttttatgaat
     1861 atattttatt attattgtta ttttattcat tctaaaatag gtaaaactca atatataaaa
     1921 atacttttgt catttaaata aaacctaaat agtttttaag attattttag acacgtttta
     1981 agtcataaca gtatcagcta ttctaaatat cgtcaaagta gccgcagttc ctgctgattg
     2041 cccatttcgt atccctactg ttgtagatga aaaattattt attgtaaaag tagtcccccc
     2101 aaataaaaaa ccagcaaaat atccagaaag tactcctccg cctcctggct cagtagtaag
     2161 ccctgccaaa tcttttcctc ctaaaattgg gaataggata gcaaaagaat attgatttat
     2221 acttgatgaa cctggttgta atgctactga aactgatata taatacacac cattttcttg
     2281 tactgtaaac gtaccatttg catctactgt tatacttgaa ctatttccta ttattgttta
     2341 ccaacctgtt atagcatttc cattatttag tgtttgtgga gatggtgcat ttacgaggaa
     2401 gttagcaatt ggctgagagg aattgcctgt tgctcctgtt ggacctgttg ctcccattgg
     2461 tcctatactt cctgtcgctc ctcttggtcc cattggacct gtaattctaa ttggaagaca
     2521 ctcacattta ggataacaat tatctataat tctatcttca aatgttacaa gtatatacgg
     2581 tttgttacaa tccctactag atgtaaattc aaaaatctga taagggtaat ttaaagataa
     2641 agctaaccca taattaggat atttattcat tgcccatgct tttatagttc ccgtaatatt
     2701 taaacaaata tagttggatg tatcacttat cccaattggt aaatatcccc tgatattttc
     2761 catagacact ctatcattcc atgttatagt tcgtaaatta taatattcac ttattctttt
     2821 aatttcaata tctacttttt ctttaaaaat atgattctta tttgaatcaa tatgaataaa
     2881 taactctgct tttaatatac ttttgtcttt taatatatct ggtattctca cttaaaaaaa
     2941 agtaatcaac ttttctttta caaaactatt tgaacattta cttcctatta agcaataatc
     3001 taaattacta aagtttttat ctggatattt tttagatata aaagtatcat catttaaata
     3061 aagtataatt tttctcattt ataatccctc cattttgggt ctatatcata ctatgtttaa
     3121 aatacatgct tagttacaat acaattaaaa tttatagtat taaattttta tataataatg
     3181 ctgatttcta caaaaaaaga acatgtcatt agaaacttct aatagcatgt tctttttcat
     3241 tattatatat tacactttaa attattcctt gactggctta gctggtgtaa actaaaccag
     3301 tctttctaaa atattaagca cttttttctt attatgtttt ttaggattta ttgtctctat
     3361 tattgtaaac caaagttata aaattgtaaa tagaatatat taccataaca atattttttt
     3421 ataagtaaaa attataaaaa accttatttt tattaaatag aataaataaa ataagtttaa
     3481 aaaatactga aataagtgtt ttccacatat tttatatatt tatttaatac attattattt
     3541 aaaaataaat atataaacaa tcataataaa atttttttaa taaaacttta ttttttagtt
     3601 aaaatcatac aatatatagc tttccacaat acaaataaat taaaaagtga aatgtatttg
     3661 tctaaaaaca taataacatt tcactttatc ctcattttta agtaatatat atttttaatt
     3721 tttatttata actcaatcta gtttttatat aagttttagt ttttttctac atctgagtaa
     3781 tctattccaa aagtttctac agtaacagac ttcataattt gtggagtttg tggcttatca
     3841 gctgaatctg ttctaactga tgctatttca tcaactgtat ccataccttc tatcactcta
     3901 ccaaaacctg catactgacc atctaagtgt ggtgagtttt tatgcattat gaaaaattga
     3961 gaaccagctg aattaggtgc catagttcta gccattgata tgactcctct ttcatgtttt
     4021 aaattgttag taaatccatt accactaaat tcacctttta ttgagtgacc tgggccaccc
     4081 attcctgtac catttgggca tcctccttgt atcataaatc ctggtattac tctatgaaat
     4141 attattccat tgtaatatcc ttcatttact aatgatacaa agtttttaac tgtatttgga
     4201 gctatatttg gatataactc aattttgatt tcttttccat tttccatttc tatagttact
     4261 ataggatttt tattttccat aatttattac cttccttttc attagttaaa ttacataatt
     4321 acatttatta tattatactc taaattaaag tctatcaagt atattttttc ctatttcgta
     4381 tccttcttga taaatagttt tacaaaactc atcatcaaag ttaagcgtat ctttaggagc
     4441 aaattgagta ttaggtctaa ctactattat tttatcttca tcatatactt ttttaatatc
     4501 ctctggcaac tcataattat attttgtgga tattaaaata attttatcca cattatcccc
     4561 aattaatggt tcaattaatg cactcctaat taaacctcca tctaatttga aaccatcata
     4621 aattccttct tgcacatctt tcacaaattg ttctttaaat ccaagagttg cgtttggatt
     4681 tgatggtaac aacccactgt atgaaatgct gataatggct tcattacgag gcatttttga
     4741 tatgtcaaca atcttttctg atactacttt attatcaact tcaacataat taacataaaa
     4801 gttcatttct tctatattac tatcatctag ttcccttagg ttgtctatag caggagaatt
     4861 atccacagta ttatttacta tttttacatt tccattctgg atatctttta tatttgtcca
     4921 cattttctcc aagttatcaa cattccctgt gtacaaataa tatccattta ttgctcctat
     4981 tgatgtccct gaaatcgaat aaaatttatt tattcctcca tcatacaagg ctttaactac
     5041 tcctgcttga taagcccctt ttgctcctcc accttctaaa cataatccta atttcatagt
     5101 ctcatctcca aattgtttat cttatattta tatttcaaaa aacttactaa atgttttata
     5161 tatactatag tggtcctcaa catttcctaa ttctcttata gagtgcattg ctaaaattgg
     5221 acttcccaca tccacagatg gtatatctat atgagtagaa gatattggac ctatagtact
     5281 tcctcctctt tcatctgacc tattaacaaa ttcttgatac tcaacattaa cttctctaca
     5341 tatattttta taaatagcta tagaatatcc atcactagta tatgcctgac tagcatttat
     5401 ctttataact ggaccttttc ccattattgg tttattagtt gggtcatgct tttctggaat
     5461 gtttggatga atagcatgag ctaaatctga tgatatcata aaagatgaat aaatagactc
     5521 aaagaatccc tctctatcct tacccattga tatgcaaatt ctctccaata tatttaacaa
     5581 catgtttgaa tctgcacctt gtttagtaga gctaccaact tcttcattgt caaatacagc
     5641 cattacattt acaccttgtt gacctttagt attaacaaga gcattcaaac tagcatgtac
     5701 cattgcaaga ttatcttgtc tcccaataga tataaattca tcattagccc ctattaaact
     5761 tcctttttca aattcatata aaaataaatc aaaatctata atatcttcca tatttacatc
     5821 taagttagat gctatttgct ttataagaaa attatctttt tctaatgtat catttaacat
     5881 cccaactaaa ggtaacatat ctttttgctt attatatgaa tgaccatcat ttatacttct
     5941 atttaaatgt attgctagat ttggtattat acaaattggc tttttaaagt ctataagcct
     6001 ttcatttggt tttaatatac tatcaccttt taaaactact ctaccagcta tcccaagagg
     6061 tctatccaac catgtactta atattgctcc tccatagcat tctgtattta acttcaaata
     6121 agatttttca gcttctattt ctgcatttgg tttaatacta aatgtaggag aatctgaatg
     6181 agagcctatt attctaaatc catctctttc tatatcttct gaatttacta caaaagctac
     6241 tatagctgat agattcttag taacataata ctttccacca actcttagat tccatctttg
     6301 attcaaaact aattcttcaa atccattttt atctaagatt tccttagtac tctcgactgc
     6361 atgaaaagac gacggactat catatatgaa gtctattaag tttttagcaa agtttatact
     6421 atccattcct catttacctc cattataaaa tatttagtgt tgtatattct ttatttattt
     6481 ttttaattcc ctgttggtca aaagctatag taacatctgt accaattata gaaaccactg
     6541 tcccaacccc aaatttagga tgatgtacct tagaaccaag ttttatatca tctggattag
     6601 tttctttatt agaatctttt attgtagcat taactttatc tgcaacttta gatttgctta
     6661 tagtactcat atacttttta gtatatttat ctaacatatt ataacttgct ttagaatagc
     6721 ttagttcttt ttttactttg tacaatcttt ctatacattc ttctggcagt tcttccataa
     6781 atcttgaagc tattgcaaca tttgttttac catataatgt tctcttctct gtcaatgaca
     6841 tatatagctc ttctttagct ctagttatac caacataaca aagtcttctc tcttcttcta
     6901 tttgtgaatc actcatagat tttatagctc ttgatatagg aaataaccct tcttccatac
     6961 ctgttaaaaa cacaacaggg aactctaaac ctttggatgt atgaattgtc ataagcgaaa
     7021 ccctatcatt gtcttcttca tcatttgatt cagatgtaag tgctatactt gttaggaatg
     7081 tctctaagtc tttattttca taagtatcat cattactact ttcaaactcc aaagctatag
     7141 aaataaattc tctcaaatta tcaattctgt cttgagcttc ttcacctttg cctgttaaat
     7201 ctttttcatt tttatttctt atctctacaa gctcgtccat atatcctgta gtatccaaga
     7261 ctttttctat aagtttgcta acaggataaa cttcttttat agttctaaga gtccctataa
     7321 tatcaacaaa tccacttata cttgcccttg ccttagtaga tatatctgaa tttgtttcta
     7381 tatcaattaa tactgagtat atactttcct gttttaagtt agctctatct tcaatttttt
     7441 ctatagttct aagccctata cctcttctag gaacatttat aatccttttt atagatatat
     7501 catcttgtgg attttgaata actcttaaat acgccaacaa atcctttatt tcttttctct
     7561 cgtagaattt tgtaccacca tatatattgt atggaatttg acttctattt aaagcgtcct
     7621 ctacaggtct tgcctgtgca tttgctctat aaagtactgc aaaatcctta taaggtctat
     7681 tttcttttct agctatttgt gcaattgaat cagcaatgaa atcagcctct tctatttcgc
     7741 taccagttaa ttgtatcttt ataagttctc cttctttttt ttcactccaa agcttttttc
     7801 tttttctttc tatattatta gatataactt tattagctgc atctaatatc acttgtgtag
     7861 acctataatt ttgttctaac tttacaacat gtacattgtc ataatctttc tcaaattcaa
     7921 gtatgtttct tatatctgcg cctctccatc cataaatact ttggtcatca tctccaacaa
     7981 cacaaatatt ttgatgttct cttgctaata attttatcaa ttcatattga gctttacttg
     8041 tatcttggta ctcatctacc attatgtatc taaatctact tctgtaatat gctaatactt
     8101 catcattagc cttgaaaagt tctacagttt taagtattaa atcatcaaag tcaagtgcac
     8161 tatttctttt taaacggtct tgatataacg cataaatatc tgcaatctta accattctat
     8221 tatctgccat gttgatatct ttaaactgct ttggagtata aagtttgtct tttgctcctg
     8281 aaattgcact tataactgct tttggttcaa acactttatc acttaaattt aattctttta
     8341 gacagtcttt aaccaatgta acttggtctg ctgaatcata aattacaaaa cttctattgt
     8401 aaccaatctt atttatatct tttcttagta tacgaacaca gcaagaatgg aatgtactta
     8461 tccacatatc cttagtttca ggacctatat tttgttcaac tctctctctc atttcatttg
     8521 cagctttgtt tgtaaatgtt atagctaata tatttggtgc ttgtacacct ttatcttcta
     8581 taagatatgc tattcttgtt gttaatacct tagttttacc tgaaccagca ccagccagta
     8641 taagtactgg accttcagtc ttttctactg cttctctttg agcaggattt aatgtatcta
     8701 aattcatatt tctcctcctg ttaacagcca taaatatatt ttaatctaaa atatgaatta
     8761 cttcaaatta taattatgta ttatattaat agattatttc caatatatta caattaaata
     8821 acatttttcc atataattta tttaattgtt taataaaaaa aataacctta tgatttgggg
     8881 aatcataagg cacctttaag gggtctaggt taagggttat tatatataaa tctatttttt
     8941 taaaataggt ttagggctta attatttatt tgtattaagt tctctttcct tccaatactt
     9001 atacaaatct tcttctttct taaataaggg taaattcata tttctatgta cttttactaa
     9061 ccatggggat tctaggccag cttgtataat gttattttca tttacaaata cattttctgc
     9121 ctttccttca tcaattaaaa tccccttatc caatacatat atataatcgc atatttcata
     9181 cattaaattc atatcatgac tggatattac tatttttata ttatctttat tcaatccctt
     9241 tattatatct acaatagacc tagtacttac tgggtctaaa ccagctgtag gctcatcaag
     9301 caatataatt tcattttcca ttgctataac tgatgctatc gctacccttt ttttctgacc
     9361 ataacttaaa aaatgtactg gtctatctat aaaatctatt ccattaacag ctactagagc
     9421 tttgtttatt ctctctttaa ttattttttc atctatacca atatttctca ttgcaaaggc
     9481 tatatcatca tatacttttg aataaaaaat ttgtttttct gggtcttgga acacaatacc
     9541 tacatttttt ctaaggttgt ataaacttct cttatcatat tttaactttt cttctttaaa
     9601 aagaatttct ccagatgtag gctttaatat acccattaaa ttcataaata atgttgattt
     9661 tcctgaacca tttgaaccta ttatacctat aacatttccc tttgaaaaat ccatattaat
     9721 atttaatagt gcattggtat tcttttcata ttgataagtt aaattattta ttttaaacat
     9781 cattatcccc tactatgtga aatttaccat catacaattt tatgtctaat gatatacaca
     9841 tatcatcata ccttgtcata agtcttttgt acagcatact gccaagaatt cctaatgatt
     9901 tatatgaagt ttttaaattt atgtacccaa atcttagttc ttgtgactta tatatttctg
     9961 aaaattcttc taaaaatatg aatataaacc tatatattaa cattgatagc tcaacaaaag
    10021 tatcaggcaa atgcattttt ttaaagaaga atattaattg attaaaaggt gttgtaagta
    10081 tacaaaagta tacacaagtc aaacaagaca tagctctaaa taacacatga atagacatgt
    10141 caatagatgc ttttgatatt cctacattga aactaaaaat ttcaaaacta tataataagt
    10201 ctgccttatt aaaagatata tttacaagag ttattcctat actaagaaat aaaaagtaca
    10261 ttggtattct gagtaatttt aaataattct ttaaatctat tccagctaca caaacaacta
    10321 gaatactcat taaaatcata attccactta aaacaaatat attttttatt accatagaag
    10381 caatcagaaa gattactcct atggctactt ttttatttgg atttacttta cttaatctat
    10441 ttgtatatgc atatttatca attataagca taattaactt ctctttcctt tttgaacacc
    10501 taatatgtac cctattacac cagcacctaa cgctgcttgt gcagagaata ataaactttc
    10561 tatttctcca cttggtggct catatgggct actaaaccat ggttcataat ctggatttat
    10621 cttagtaatt tcactctcag cttgaccatc tgctcctcca tattcagctc ccgaattaac
    10681 aagtaatggg aatatgataa gagctactac taataaaagt agtaatatat tagttttcgt
    10741 ctttgtttta gcactcatta gctttaactc ctttatcatt attttctagt atgctttttt
    10801 ctccattttc tgtaattaaa ttatatacaa ttacagtaag aataccctct gctattgcta
    10861 ttggtatttg agtcataaag aaaattccta aaaacttaat tgctgaagcc atcactccac
    10921 ctgatgggtc tgggaaagcc agtgctaact gtatcgaagt tactgtatat gtagccaaat
    10981 ctcctattgc tgctgcaaga aatactggca ttgcattatt tccgtctttt ttctttaatg
    11041 ctttaaatat taagaatgat ataattggtc ctataatagc cattgaaaaa gcattagctc
    11101 ctaaagtagt tattcctcca tgtgccaata gtaatgcttg gaatattaga actatagttc
    11161 caagaacaaa cattacactt ggtccaaaca ttattgctcc aagacctact ccagtaggat
    11221 gtgaacaact tcctgttact gaaggtatct ttagtgctga caatacaaat acaaatgctc
    11281 cacacagcgc taataatact ttctttttag ggtcttgctt tactattttt cctattgatt
    11341 ttagtcctac taaaaaaaat ggtataaata taactcccca tgctatagac cattttactg
    11401 gtaagtatcc ttccattatg tgcattgcat tcgatgtaac tggagttaaa acgattaaca
    11461 taagtgcagc tataacccct aatttgatat tttgtttcat tttactatcc cctctctccc
    11521 tttctaataa ttaaaatata aataatatat gtctgattta ttttaaaaat aaaaataaaa
    11581 aagcctaact cgatggctag gcttctaatt ttcgataatc caaatacaaa cacataaaat
    11641 gaacatatct gtataattat tataagaaac tttctccatc cccgaagaaa ttactttaat
    11701 attttaggca ggtctcctga cttagcttct tcctactcct cttccttccc atgagatata
    11761 aaaaatatca tcatagtggc taacaaaagt ttcgtctgca tcacagtagc gggggctgta
    11821 aaggaattta acctttttcc ctattaatct tgcttaaaga acctataata ttatatattt
    11881 aatttttaaa ttattcttaa atcattttct atataaatca ttgtatacct tttctgtgaa
    11941 aatttctaca ttttttataa attttattca tttttatatt tttatgtata taaattcttt
    12001 tttgtaagtt tattaatttc taattatctt ctttaaatac attgaaacta catcagaaaa
    12061 tctagttata tctcttgaat atataaaatc atttccatat attaatcttt cagcttcaag
    12121 ttctgattct tttcctgtaa atactcctag tactaatata ttttgctttc tagcttttct
    12181 cacttcattg gctgtatctc tcaaaccaac tataccccta taagcctttt ctcctcttaa
    12241 tgttctctca ctactttttc ctatctttac atcatttggt cttccatcgc tgagaactat
    12301 caggatttta ttttcttcat ctctatttat tagaccacta catatagatt ttattgccaa
    12361 accatctcta ttattaccag cacaaaaata ttcaaatata ttttcactat tttttatact
    12421 atcatcataa tctttaaatc tttttaatat tgtatagtct aaaaaactag aaaatcccat
    12481 cactctattt ggtatgccaa cttctgttag agcatttgca attatgtaag cttgaattgc
    12541 cacatttccc tgatttctac tttgagagcc acttgcatcc aataatatat caataacgta
    12601 tttccctttt tcattttcta tatctttata aaatatttta ttattattac ttctaccaac
    12661 tctccaagct ctatttgcta atatagtacc acaatcagaa tatactctac ttttagcatt
    12721 ttcttctatt aaaattcttg acagactttc ttttaatctc tttatttgct gtctatacat
    12781 tctagcatgt tctctaaatt ttcctaaatt attttctttt tgtcttgtta catattttag
    12841 ttgaaataca ttattacact cacttctaag aactccatct gtaaagtgta ttctacaacc
    12901 ttcatgaaca tttctacaat tttgtgtctc aattttttta atttcacctt tactcaaata
    12961 agtttttcca taataatgtt ctatttttga atatatctta tttgcagtgt cttcatcaac
    13021 atatataact cggtctgctg aattgctaca tttagaatca tcaaaatccc ctacaccttc
    13081 tacaagcatg gatgaagata ttgaatctat atcactttca ataatatttt cttcttcatc
    13141 actataaagt tcttcataca tgaaatcagc aaatgtatca aaatttactt ctacactttc
    13201 tacattattt tcatcaataa gactattttc ttctccattt aatatgtatt cgaaataagt
    13261 taaatatata acatctaatg cttttaaaat ctccatcata tcttgattgg tatctatatt
    13321 tcttatatca ttaactattc ttctagtcat actatctaat gctgggtgct tgtccatatt
    13381 ataaagtaca aatgtgtatt taattttatc cataatatta tcattgtgta ttttggtaaa
    13441 acgtgaaaca atatctttat aagcatttct tcttatgtct tctatccctg gcctatcttt
    13501 aatgaccttt tcttcaacta ttgtatttaa tataacttga attagaccca gtataatatc
    13561 tttgttgtat ccttttctta ttctatttgt aagatatttt ttaactatat cccaatctac
    13621 atactttctt ctagcacctg caattattgc aaaatataga cctatttctt ttgaagagta
    13681 atctttttct gatatatcta tactttcatt gtaatcacca gctattgtcc atgataagtt
    13741 attagctcta ttttcaaatt cataatcatc atatatccat ttcataatct cacctatatt
    13801 aaatatgact tattggaaat acatctatac tctcccattt attaggtatt ctagttttaa
    13861 ttacatctcc aaccatttct ttctcatata catcaaaagt ctttcctgta agtcccatat
    13921 taattgcaag agttggtttt aatcctcttc ttatagtctt aagagatgcc ataagaccac
    13981 gtaaatcaat agctttacta gatatctctc cattttgaga cttcaattgt aaatctaaaa
    14041 atattcctgc aaagtgtatt aatttttctt catcagcatc tgaaaattca ttttttaaaa
    14101 tcatcattaa cttatcttct tctataggag gaatatctat taccataaat ctagatacca
    14161 gtgcttcatt aagttctttt gttcctgcat attcataatt catagtccca ataaatcttg
    14221 ttgctggatg tagattgact ctctcatacc caggtacatc aattattctt ctatagtcta
    14281 aagctgaatg taccacaact atagcatcat tttttgccat attaatttca tcaaatactc
    14341 caaaacctcc attaatagca cactcataaa ctgagcctct tctaagttta acttcattgt
    14401 ctataaatgt atctgtacct ataagtgttg aactgtctgt atttatatga aatgatgtat
    14461 tccactgagg tctattaaat atttccccta gattatcagc aagtaaattt tttcccgttg
    14521 cctttggtcc cgataataat atattttctt cttctaaaat agctgctata cacatagaca
    14581 agatatcttt accataaaaa taagttttag actttgttac tctgtattct aaatcttttt
    14641 ctaaaccata ataatttcta aaatacagta catcttcaat aagtttttca tctacccctt
    14701 gcacccttag attatctatt atatccatat ttttacccct tttatataaa tagtatttgt
    14761 ttcatttctt atcaccattt tagcataaaa ggatataagc taaaattgat tttttaatct
    14821 cccccatttt aagtaaaacc atgcaccatt ttatcactac aaaattaaga gtactactta
    14881 ctgatatttc ttgattttgt tcgtctatac gtatataata tatgctgata aattatgagg
    14941 aaaggatgat tgaaatatgg aatatatgtc ttgcccagaa gtagcaaaaa aatggggcat
    15001 ttctgaaaga agagtacaaa aactatgtga agaaagtcgc ataccaggag tttcaaaact
    15061 cgggtatatg tggctgatac caaagaacgc agaaaagcct gttgatggta gagcgaaaag
    15121 aagaaaggaa gtacaatatg aatagcgttt tgattataga tgatgacaaa gaactttgtg
    15181 ctcttatgaa aaaatgtgtt gagcaagaaa atttatctgc tattactgca cattgtggtg
    15241 cagaaggtct acatttgctc gataaaaata gacacacact ctctcttgta attttagaca
    15301 taatgatgcc agatatagac ggttttcaag ttctgaaaaa aattagagaa acaagtaata
    15361 ttccagtaat tatgcttact gctaaaaatg acgaggatga taaagtttcc ggactacgtt
    15421 taggtgctga cgattaccta acaaaaccgt tcagcataaa cgaacttatg gctcgtgtca
    15481 attctcttat tcgtcggtat actatgttaa accctgcttt tataacagac acagattgca
    15541 taataataaa tggcatgatt attgacaaga caaaccgaat tgtttctatc aataatattc
    15601 ctgttcaact tacaagcaaa gaatttgatt tacttgcttt tctggcttct aataagggac
    15661 gggtatttac caaaaaacag atttatacac aagtatggga agaagaatat gcttttgacg
    15721 atagtaacat catgtctttc atcagtaaat tgagaaaaaa gattgaacca gaccccaacc
    15781 atccatttta tattttgaca gttcgtggtg tcggttatcg ttttaataag gaggcttaac
    15841 atgaatacaa gtgtctatct acttttgata ttggctctta gcctgattat tatagcgtac
    15901 cttgtttctg tcctgctacg tgtccgaata caacttacac ttataaaaga tgctttagag
    15961 gatataaaaa atggaaacct taatcgccgt attttaacag atgaaaacga cattacaaaa
    16021 caaatttgct atgatattaa cgaaatagca atcaatagcc aatttcaact tattcagcaa
    16081 aaacaatcag aacaagccta caaacagcta atgacaagtc tctcccatga tgtaaaaact
    16141 ccacttgctt ctttggtcgg ttatttggaa gcagtagaaa gtaaaattgt tgtaggagag
    16201 gaaaaagacg agtatatcca tgttgcgtct aataaggctc attacttaaa gaattttgta
    16261 gaaaatctat ttgaatgggt aaaattggat tctaaggaac aagtttttca ttttgatatt
    16321 tttgacttga atgaattatc ccgtaatatt atatctgatt ggatacctgt tttagaaagc
    16381 agtcattttg aatatgaatt tgatatacca gaaatagagt acttcttacg tatagacgca
    16441 aatgcatata cccgtatcat taataaccta ttacaaaata ttattaccca tagtagtggc
    16501 aacaaaatga cattgagaat ttttgaaaac aaagagcaag ctcaaattat aataacagat
    16561 aatgggaaag gtatatcttc agacaatctc ccacatattt ttgaaaggtt gtaccaatgc
    16621 gaccattcac gagcttcaaa gggaaatggt ttaggcttgg cgattgcaaa ggaacttatt
    16681 aacgctcata agggaaatat taccgccaac agcactcccg gtatgggaac agaatttaca
    16741 atattgcttc ctaaagccct gtaaaagtac gaggctttct tactctaaaa caagataaaa
    16801 gcaagattat ggcaaggttt tggcaaggtt catgttttat aattctaatt gtagttgatt
    16861 ttgacgttgt atcaactatc ttttgtagaa acaaatttat taaaagtgag gaaattatat
    16921 atgaatgatt ttgtgattga aacaaagcaa cttacaaaaa tttatgggaa tcaaacagta
    16981 gttgacaaag tcaatctcca tgtaaaaaaa ggccgaatat atggtctgtt aggacgcaac
    17041 ggagctggga aaaccacaat tatgaaaatg attttaggat tgacttcaat cacgtatggc
    17101 gaggtagatg tatttggaga gaatattaag ggatgtgaaa aacgagtata tccccgtatc
    17161 ggtgcaatta tcgaaacgcc aggattttat cctaatctga caggaacgga gaacttggag
    17221 atttttgcaa aacttcgtgg tacagccgcc cctaattctg taaaaagagc attggagatt
    17281 gtaggcttac cttataaaaa taaaaagctg tttagtaaat attcacttgg catgaaacag
    17341 cgacttggta ttgccaacgc tatcctgcat gacccggaac tgttagtttt agatgagcca
    17401 accaatggac ttgaccctat tggtattgcg gagatgagaa agtttattag ggacttgagc
    17461 gttgagcatg gaaagacaat tcttatctcc agtcatattc tttcagaaat ctcattgttg
    17521 gctgatgata ttggaattat cgaccacggt gttcttttgg aagaaagtag tatgagtgaa
    17581 ctagaaaaga aaaacagtaa atatatcctg ctacaagttt cagatattcc aaagacatca
    17641 cttattttag agcgtcaatt taacacgaag gattattccg ttcaagatga ccacacttta
    17701 cgcctttata acactactct ggatatgggt gaaatcaata aagcacttgt aatgcagaat
    17761 gtaactgtta ttagttctca actttgtaat gatactttag aggactactt taaaaaaatc
    17821 acaggaggag agggcattgc ttaaacttat tcaaacagaa tttcttaaac tgcgtcgtag
    17881 gaaacttatt tggctgatgt tgttgtctgc tctcattatg ccgtttttcg cactactata
    17941 cttcaattat tttggtaaaa caggtgttga gccaacaaag ttttataaat ggtctgcatt
    18001 tggttataca ctttggatta ttttgcccgt tgttttgggt atattttgca ccatgcttat
    18061 acacgaagaa aaccagtatg atatgctcaa acagctttgg attgtaccta ttagtaaaat
    18121 gggatacttt tttagtaaat ttttcgttgt tttgatttac tctatttgtt ttatgctgat
    18181 tacagccgtt gcttctgtgt tatttagtgt actttctgga tatgtcgtat ttagctgggc
    18241 aagcatttta tatttgttga aaaaatgttt agaaattggt gtaatcactg cttttgtaat
    18301 gttgccgatt ttggcaatcg ctacatctca aaaaggatac attctcccaa tttgtatcac
    18361 attggtatat gccttttccg gttttgttat aatgacaata aatatgtatt tacacccatt
    18421 atccagtatg ggagttatta tcatgagaaa taaagacatt ccaggtctta tattttcaca
    18481 ggaaattaat gtcccattag catttctttg tatttgtatt tggagtattg tatcagtatt
    18541 gtttgcaagc attgcactag gaagaagaaa gtgaggtgta gataatgtgg aaattacttt
    18601 gggctgaatg tcaaaaaatt cgtcgttcaa aaattgtttg gattacattt tttgcatcca
    18661 ttatgatagc agtcatagtt tttgcaggag ggcaggatgt atataatgac ccagatttac
    18721 attatggctt aaagagcgtt tatgacggtt cacggtatat agacaatgca ggttggtata
    18781 tggacgcggt acaaccttgg tcaacttttt ttgttcttcc tgctgtcatt gccctattag
    18841 gaagctatat tatttgtcgt gaagaagagg aggacactat taaatccctg cgtcttattc
    18901 ccataaatga aacaaaatta accatagcaa aaatgattat tacttttata ttcagtatac
    18961 ttctttattt gttactcttt actataacat ttttgactga atccgtttta catttttccg
    19021 atttatcgac aaaactggtt ttaagttgca tgaaagagta ttttcttgat ggcataggtg
    19081 tattctttgc aatttctccc attattgcct tggtatcgcg catgaaaaaa ggatattggc
    19141 ttgcattagt attcacagaa gtttattcta ttgctggact atttgcgggt atgtcaaatg
    19201 tcttacaaac cttttttcct attgcagcaa tatttaatat ttctggatac tatataacta
    19261 ctggagaaaa aattatgggg agtatcataa gtttattact ttgtgcctgc ctttctgcat
    19321 ttatactgaa agggctaaaa cacaatgaaa aaaattgaaa taggtatatc aaaatgaaaa
    19381 agataaaaaa actgattatt ctaattgtat tcttgtttct ttttggcatg ttacactgtt
    19441 atactgaatg gaaattcaac aatgacccac ctaaaggggt aatagaaaga ataaatgagt
    19501 ggactgataa gattggactg gattagtaac ttatgttaat tatagtgaat gtaatattag
    19561 caagttattg tttcgtattt taatactata actttaaata tttggtttta atcttccgaa
    19621 cgaatataaa aaaactgtca cagaggcaac atatttttga atatttataa agtggtagtt
    19681 ttgattttca aagctgccgc cttttcttct atataagcaa acttatcctc aacaaagtgt
    19741 aaaacatggt acattttaag aaaaaagtac cacagacaca agcaataaaa gaaacgccga
    19801 gaacctacaa aaacgtagtg cttatgcaga tttacgacgc cttctatttt ctcaaccgtc
    19861 ttaatgacct tgtacaaaag aaatttttct aatatttttt ataatcttat cttgtgatgt
    19921 tttttatgta taaatgactt aataacaaat ccaataaaaa ttatggatat tggtataatt
    19981 agaacgagga atttataatc attataatgt gacattattc ttacccaact ctcagaaaaa
    20041 atatatccta tactgattaa tgaagtattc catatggtta ttcccacgat agatgatatt
    20101 acaaaagtta taatattttg acctgctata cctgctataa aagatatgta tgttctacat
    20161 aaagggatta atctacatac acaaacagaa atagaagagt atttattaaa ataatcttga
    20221 gatgcaaata ttcctttctt tgcctttgga catatctcta ttattttatt tatgatagat
    20281 ttacctcccc aatttcccac tgtataacaa aatatcgaac caagtatacc tgcaattata
    20341 cttaaaatta aaattgtcag aaatccaaaa tgatttcttg cagcaattgc tccacataaa
    20401 ggtaaaacaa cttcgctagg taatggaaaa caagcatact caatcataat taatacaaat
    20461 acagagatta atccatattg taaaataaaa ctattgatta cagacactta cttatctatc
    20521 tcctttctta tttaaaggtt ttaagggtac atattctaag gattctcacc ctatatttaa
    20581 agaatcaatc tctttaaact attatatgta tttacacaaa gcatttataa catactttaa
    20641 aaatagtacc ttaaaataag aaacttaata aataaaaaaa gattacgtgg ctacgtccta
    20701 ctctcccagg cag
//
LOCUS       DAMJVX010000406        16248 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.guided.081, whole genome shotgun sequence.
ACCESSION   DAMJVX010000406 DAMJVX010000000
VERSION     DAMJVX010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16248
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.guided.081"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            247..864
                     /locus_tag="PC407_003866"
     CDS             247..864
                     /locus_tag="PC407_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF2499229.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIITPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            972..3503
                     /locus_tag="PC407_003867"
     CDS             972..3503
                     /locus_tag="PC407_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2499230.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVKEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3937..4638
                     /gene="vanR"
                     /locus_tag="PC407_003868"
     CDS             3937..4638
                     /gene="vanR"
                     /locus_tag="PC407_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HDF2499231.1"
                     /translation="MNENILIVDDKKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4688..5770
                     /locus_tag="PC407_003869"
     CDS             4688..5770
                     /locus_tag="PC407_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2499232.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            6027..7127
                     /gene="vanG"
                     /locus_tag="PC407_003870"
     CDS             6027..7127
                     /gene="vanG"
                     /locus_tag="PC407_003870"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2499233.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            7124..7930
                     /locus_tag="PC407_003871"
     CDS             7124..7930
                     /locus_tag="PC407_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2499234.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7948..10086
                     /gene="vanT"
                     /locus_tag="PC407_003872"
     CDS             7948..10086
                     /gene="vanT"
                     /locus_tag="PC407_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF2499235.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            10400..11020
                     /locus_tag="PC407_003873"
     CDS             10400..11020
                     /locus_tag="PC407_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF2499236.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(11186..11722)
                     /locus_tag="PC407_003874"
     CDS             complement(11186..11722)
                     /locus_tag="PC407_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2499237.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(11835..12509)
                     /locus_tag="PC407_003875"
     CDS             complement(11835..12509)
                     /locus_tag="PC407_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF2499238.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            12713..14587
                     /gene="asnB"
                     /locus_tag="PC407_003876"
     CDS             12713..14587
                     /gene="asnB"
                     /locus_tag="PC407_003876"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2499239.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEYVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(14679..16115)
                     /locus_tag="PC407_003877"
     CDS             complement(14679..16115)
                     /locus_tag="PC407_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2499240.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVHFTRY
                     IGVDDSIDKYIEKVKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWM
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIAQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
BASE COUNT         6201 a         1765 c         2711 g         5571 t
ORIGIN      
        1 tcaaaatgaa ctttttagtt catgaggcac tctttttgta cgaaataaaa tatattttta
       61 ttatttcaac aatgaaaaag atgcttgttt ctattttgag acagtataat ttttatgtaa
      121 ctttaatttt agtaaatata aagttttaaa attgataaaa taggaggaat ttttaatata
      181 aaagggtaaa taaagggttt agagggcata atatagaata aattaatata tttaaggagg
      241 atttttatgt gtaaaaaaac gtatttatta gtggtaagta tacttatagc aggtttatta
      301 actgcatgta gtgggagccc aactaaggat tcaggacaag cacaagataa tacaaaaaag
      361 gaggcaaatg cttcaaataa tgctttaaaa gatgaaaaaa ataatgaaaa tttaatggaa
      421 caagatttta aagtgccata cacagatgca ataaacatat ttaaagataa atacaaggat
      481 gctgatatag tagatttaag tcttgagaga gatttaaata aatttgttta tactgtggaa
      541 ggtgtagatg ataataatga atataagatg aaaattgatg ctaatacaaa agatgtatta
      601 gaagataaaa cagaaaagtt agattcagaa gatttaaatg gagttgctag aaaagaaaaa
      661 ttagatttaa atgacataat tacacctcaa caagctatgg aaattgcatt aaaagaacaa
      721 aatggaatgg ttaaagaatg gagtttagac aaagatttag atgttacttt ttataaaata
      781 agaatagata aagataaaaa tgaatatgac ataaaagtag attctaaaaa gggaactgta
      841 ttgaaggtag aaaaagaaga ttagaagatt taaactatct ttaaaataat ttcttttttg
      901 aataaatttt gtttaaaata taacaagcag ggtataaaga gactaaataa ctattttagg
      961 aggagtacgg tatgtcattg aaatcactaa aaataaaaga aaatttatat tgggtaggtt
     1021 cacttgaccc tgatttaaga gtatttgaca taattatgta tacaccttat ggaactactt
     1081 ataattcata tgtattaaaa ggaacagaaa aaacagtttt gtttgagaca gttaaagata
     1141 agcattttga caattatatt gaaagattaa atgatttaaa tatagatttt gaaaaaatag
     1201 actatattgt tgtaagtcat actgaaccag accatgcagg aagcgttgaa aagcttttgg
     1261 atttagctaa aaatgcaaaa gtagtagctt cagaaacagc aattaaatat cttaaggaaa
     1321 tcgtaaacaa agactttgaa tatgttgcag ttacagatgg agatacatta tcaattgggg
     1381 acaaaacttt agaatttttc tctgtaccta tgcttcattg gccagatact atatacacat
     1441 atataaaaga ggataaaact cttgtaacat gtgattcatt tggtagtcac tatagtaatg
     1501 ataagatagt aaatacactt gatgaaaatg aagaaaagga ttacttggat gctttaagat
     1561 actattatga ttgtataatg ggtccattta aaccatctat ggtaactgct atagaaaaga
     1621 taaaagattt agatatagat actgtatgtc caggtcatgg tccagtatta actgaaaatc
     1681 ctagaaaaat tatagacctt tattataact ggagtgtaaa ggaacaaata aagttagaaa
     1741 aagaagttac aatttgttat gtttctgcac atggatatac aaagattatg gcagaagcta
     1801 taaaagcata tattgaaaaa aatagcaact ataaagtaaa tttatttgat gtaatagagc
     1861 ataagcaaga agatattttg gcaaaaatag cagtatcaca aggtgtatta tttggaacac
     1921 caacaatatt aggagatgcg ttaaagccta tatgggatat attaatatcg ttaaacccag
     1981 ttcttcatgg tggaaaagtt gcttcagtat ttggttcata tggatggagt ggagaaggta
     2041 tagaaaatgc tatggagaga ataagtcagc ttagaatgac agcagtaaaa ccttttgctg
     2101 ttaactttaa accatcaaat gaagagattg ataaattaca ttcttataca ggtaaattcc
     2161 tagataagtt aaattctact tttggaagta aaaagaaaac taagaaattt aaatgtgtaa
     2221 tttgtaatga agtttttgaa ggtgatagtg ctcctagtgt atgccctgta tgtggagcta
     2281 aggaagacca atttatagaa gttgaagaag atgaagttac ttttagaaaa gatactgatg
     2341 aatatttcgt aatagtagga aatggagcag ctggttttta tgcagcagat gctataagaa
     2401 aaagaaataa aacttgtaag ataactatga tttctaatga agatgagttg acttactata
     2461 gacctgcact ttctgatggt ataaatgaag aacttggttc agatttctac atggaagata
     2521 aagattggta tgataaaaat aatatagtag taatattagg aacgaatgta gataaattag
     2581 atgaagtgaa taaaactatt atagtgaatg atggagctat taaatttgat aagcttgtaa
     2641 tagctacagg aagtagaaac tttataccac cgattaaggg ccatgattta gaaaatgtgt
     2701 ttactcttag aaatataaaa gatttatata gtgtaaaaga agcactagaa aaatctaaga
     2761 aagttgtagt gattggtggc ggtttattgg gacttgaagc tgcatgggaa tttagactaa
     2821 aaggattaga agtagtagta gttgaagcca tggatagcat actatcaaaa caacttgata
     2881 aagagggtag taaaatatta gaacaatgtg taagagatac aggaatagat gtaagattgg
     2941 gtgtagcagt tgatggtatt gaaggtgatg gaaaagctca aaaagttgta tttaaagatg
     3001 gagatagtgt tgactgtgat atggtagtat tttcaattgg tgtaagagca aacactcaaa
     3061 tggttcagga tacatctgta aaaattgata gaggtattgt tgtagataag acattacaga
     3121 caaatgttaa agatatatat gcttgtggag atgttgcaca agtgggaaat attagtttag
     3181 caatatggcc atcttcagta gaaatgggaa aaatagcagg agcaaatgca agtggagata
     3241 atttgacatt tgaaagtgaa gtatatccag tttcattaga tgcaatgaat gtaaaagtat
     3301 ttagtattgg taatatacaa aattttgata aagaaatttc atcaaaagat gaaggtcaaa
     3361 gaatatataa aaaattattt atgaaagatg gttcattagt tggagctata cttataaatg
     3421 atttaagctg tactgttaaa ttgataagat taatatctga aaaaggtgat tttgaggata
     3481 ttatgaaggc tgatatacta taatttaaat acattaaaat aaaaaggagt tgtctcaaaa
     3541 ttgatttttt tctaatcaaa ttttaaattc aactcctttt ttgaattttt atataaaata
     3601 acatggaaat tttagtaatg tctgtggtta catacatagg tttaattgtt ttttatatgg
     3661 attgtctctg gtataataaa ttgaattgtg tttgactgat agatattttt attgaaagtt
     3721 aatacctgtt atgcgataat aaggacgtgt taggggagtt tttataataa caattaatct
     3781 cagtttttgt gttccaatag tagcatcaac acattctgat gtagtagttc ttttcttata
     3841 aaaattagtt acaaatatgc ttgtaattat ttgatatgta aatttaccaa tattataaat
     3901 gatgacctcg ataataagga gagtgaaaaa taatctatga acgaaaatat tttgattgtg
     3961 gatgataaaa aagaaatagc agatttaatt gaagtatatt taaaaaatga aggttttaca
     4021 gtatacaagt tttatacagg taaggaagct ctagaatgta ttgaatctaa agatttggat
     4081 atggctattt tagatattat gcttcctgat attgatggat ttcatatttg ccagaaaatt
     4141 cgagaaaaat actattatcc tatcattatg ttaacagcta aaattgaaga tatagacaag
     4201 ataatgggtt taaccatagg tgctgatgat tatataacaa agccatttaa ccctttggaa
     4261 gttgttgcaa gagtaaagac acaattaaga agatatatgc gatataataa ttcttatgaa
     4321 caacagtcga taattgttaa tgagtatgat attaaaggat tgattatcaa taaagaaaca
     4381 cataaatgta gtttatttgg gaaagaagta gcattaacac cgattgaatt ttctattctt
     4441 tggtatttat gtgaacatca aggaaaagtg gttccttctg aagaattgtt tgaagctgta
     4501 tggggagaaa aatatcttga taataataac acagttatgg ctcatatagg gcgattacgt
     4561 gaaaaaattc atgaaccttc taaaaacccg aaatttataa aaactgtatg gggagtaggt
     4621 tatacaattg aaaagtaaaa atattaaagt caatcctgac tttactcaag tgagaaataa
     4681 aatttttatg agaacagctt taatggcatt tatagccatt attttcatat ggattttata
     4741 ttcttttata ttgtatggta agttttctat ctggatagta tcattctttg aaaatgcttt
     4801 tcgtatggaa tataaagctg cattgaattt atatcagcag attttccgta atagcatgga
     4861 gctcttcatg atactagcta tggtgattgt gttttttata atattccgtg tttatttaaa
     4921 ttggtttaca aaatatttta gtgaaataaa tcaaggtatt gactctctta tcaaagaaga
     4981 tgttggagaa gtggctttat ctccagagtt attggcaata gaaaagaaaa taaactcaat
     5041 taaacatata cttgaacaac gtaaatttga gacacaaatg gcagaacaac gtaagaatga
     5101 acttattgtt tatcttgcac atgatttaaa aacaccactt acatcagtta tagggtatct
     5161 gacactactt cgagatgaaa gtcagatttc tgaagaactg cgaaaaaaat atttatctat
     5221 ttcgttggat aaggcagagc gtcttgaaga tttaattaat gaattctttg aaattacaag
     5281 gtttaattta tcaaatatat cacttgaata cagtacagta agcttaactc gtatgcttga
     5341 acaactaaca tatgagttta aacctacttt gatgagtaag aatttaaaat atacactgaa
     5401 tgttacacca aatatgatga taaggtgtga tgttaataaa atgcaacgtg tatttgataa
     5461 cttattacga aatgctgttt attatagttt tgaagatact actattgaaa taacagctgt
     5521 acaggaggat gattatataa agcttaaatt tataaatcat agcaatacga ttccagagga
     5581 aaaacttgag cgtatttttg aacaatttta caggttggat acatctcgta attcaaacag
     5641 tggtagagct gggttgggac ttgctatagc taaagaaata gtggagcttc ataacgggac
     5701 aataacagct catagcgaga acaatataat tacctttaag gttattattc cgattgtagg
     5761 aaatttgtaa gaatttaagt ataaaaaaat aggaaattct ttaggataaa taaagagcac
     5821 tcttgttatt gatataatta ctgtatcaaa acaagagtgc ttttattttg atacagtaat
     5881 tatatcaata gaaaaatgtg aaaatatatt atgtggaaca gatgcaattg taagtgaagg
     5941 ttgattggat ttgaaataga aaaagtattt taacatgttt ctgtagattg tataaaaata
     6001 aataaatcaa caaaggagaa tcaaagatga ataataaaaa aattgcagtt atatttggtg
     6061 gaaactcaac agaatatgat gtatcattac aatcagcttt tgcagtactt gagaatttga
     6121 atacggaaaa atatgatatt ataccaattg gaattacaag ggagggtaat tggtatcatt
     6181 atattggaga ttataataat atccagaata acacttggtt tgaaagggat actgatataa
     6241 ttccagttat tgtttcgcag aaccgtgtca aaaatggtat tattgaattt ttaattcctg
     6301 atgctgtttc tcagaatcat attccaaata gtgctattga atcatgtgaa aatgatatta
     6361 aatacactaa acttgatttt gtatttcctg tattgcatgg aaaaaatgga gaagatggaa
     6421 cagtgcaggg gttatttgaa ttggcaggta tacaggttat tggatgtggt actctttctt
     6481 cagcattatg tatggacaaa gacaaatcac ataaacttgt tcattcagaa ggtattaaag
     6541 taccaaaatc aatattacta aaatactcag ataaatttaa tgtggctgaa ctgtcaaaag
     6601 acttaactta tccattattt ataaaacctg tgcgtgcagg ttcatcattt ggtattagta
     6661 aaatttataa aaaagaagag ttacaagtag cagtagactt ggcttttgaa catgataatg
     6721 aagtaataat tgaggaaaat attgatggtt ttgaggtagg gtgtgcagtt ttaggaaacg
     6781 atgacttact tgttggaaga gtagatgaaa tagaattgtc aaaaggattt tttgattata
     6841 cagaaaaata tacattgaaa agttcaaaaa ttcatatgcc agctagaatt gatttggatt
     6901 gtgagaaaag aatacaagag acagcaaaaa ttatttatag aactcttgga tgttctggat
     6961 ttgctagagt agatatgttt ttaactccat ctggtgaaat tatttttaat gaagtcaata
     7021 caattcctgg ctttacttct catagtcgtt atccgaatat gatgaaaggt attggactgt
     7081 cttttgaaga aatattggat aagataattg aggtgtacat aaaatgatga ataatatttt
     7141 attagataaa aataaagaag ataaaatgac aaatactatc ttattgacta aagataaagt
     7201 ttatgatggg aatctaattc tagtaaatgc ttttttacca gttaaaacat ctgaagatat
     7261 agatttaatt cctgtagata caaggtttcc aagtattctt atgaaacgtg aagctacaaa
     7321 tattttacaa aatattttaa aaagtatatg tggaataaat gaaatagtac ctgtaagtgg
     7381 atatcgtact gcagaggaac aacaagatat atattcaagc tctctgcgtg acaatggaaa
     7441 agactttact aaaaagtttg tagcacttcc aaatcacagt gaacatcaga cagggcttgc
     7501 tattgattta gggttgaaga aagaggttat agattttatc tgccctgaat ttccttataa
     7561 tggtatttgt aatgagttcc gtaaggcagc actacattat ggttttatag agcgttatca
     7621 gtgtaaaaaa gaagaaataa caggaatatc tcaagaacca tggcattttc gttatgtcgg
     7681 atatccacat tctgagatta tcaataaaat gaatctcgcc cttgaagaat atatagaata
     7741 tattaagaga ttttcgtata caaattgttt aaaaatagaa gataaagaaa aaattataga
     7801 aatcttttat gtgcctattc atactaaaga agaagtgaga atctctatac ctgaacaatg
     7861 tgtatatcaa atatcgggta acaataagga tggttttatt gtgacatttt ggagaaatag
     7921 aaaggactaa gaaatggagg aacaagaatg attaggaata aatcttatgc agggcttgat
     7981 tattttagga ttatagctgc attacttata attgcaattc atacatctcc attattatca
     8041 tatagtaaaa tggctgactt tgtgttaact agtataattg ctcgtattgc tgtgccattc
     8101 ttttttatgc tatcaggctt ttttctaatt tcaacatata attataattg taataaactg
     8161 aaatcttttc taaaaaatgt attatttatt tacgggatag ctattgtaat ttatattccc
     8221 ttaaatattt acactggata tttttccatg aataatttag tgcccaatat tattaaggac
     8281 attgtatttg atgggacttt atatcattta tggtatctac ctgcatcaat tattggtgca
     8341 agtattgcat actttcttgt aaaaaaacta ggtttaaagt actcattagt aatcactatt
     8401 gttatttatg taattggtat gtttggtgat agttattatg gttttttaga aaaaatacca
     8461 ctattaaaga atttatatgg gtatattttt gaagtatcag attatacacg aaatggtata
     8521 ttttttgcac ctgtattttt catacttggt ggaataatat ctaataaatc taattatatg
     8581 tcaactaaaa atagcttgat tggatttctt atatcattct tacttatgtt atgtgaggga
     8641 atgtttttac ataaactagg ggtacagcgt catgatagca tgtatattat gttattgcca
     8701 tgtatgtatt tcttatttac agccttaaca tttttaaaag gttctcgagc taggttattg
     8761 cgaacatcat cattaattat ttatattata catcctatga tgattgttgt tattcgtatg
     8821 ttttctaaaa tattcggaat acaaactgtc ttggttgaca atagccttat tcattatata
     8881 gtggtaagta tagcatcttt tatagcttct ataattatta tattgattct taaacctcta
     8941 aaaaaagact tgaaaggaga acataaggta aataccaata gagcttggat tgaaattgat
     9001 ttaaataatt tggaacataa tgtaaagatg atacaaaaag ctatgccaga agattgtgaa
     9061 ttgatggcag ttgtaaaagc taatgcctat ggtcatagtg cttttgaagt agcgacttgt
     9121 gcaaaccaaa taggtgtaaa agcatttgca gtagcaacaa tagatgaggg gattgattta
     9181 cgtcgatatg gaatactagg agaaatattg attcttggat atactaaccc aataagggca
     9241 aaggaacttc ataaatacaa tcttacacag agtgttattg accttaatca tgctatctct
     9301 ttaaacaaac agaaatacaa cataaaatca cacattaaaa ttgatacagg tatgcacaga
     9361 ctgggttttg atgttaatga tgtgaggtca attttaaagg catttaaatt aaaatactta
     9421 gatatatgtg gcatatttac acacttgtgt gtttcagata gtctgaaaga tgaagatgta
     9481 gactttacaa ataaacaaat tgaatgtttt tataagttaa ttgattttct gttaaaaaaa
     9541 ggtataaaaa ttcctaaagt acatattcaa agtagttatg gtttttttaa ttatcccaat
     9601 cttaagtgta attatgtaag aattggaatt gcattatacg ggatattaag ttctccaaat
     9661 gatacgacta aattgcaact tgatttacga cctgttttat ccttaaaatc acaagtaatt
     9721 cttattagaa aaatacaaaa agatgatagt tttggatatg gaagagtctt tattgcaagc
     9781 agagatagcg taattgctat attatctatt ggctatgccg atggtatacc tagaaattta
     9841 tcctgtggaa aaagttacgt aataataaat ggttgtagag cagcaatagt gggtcgaatt
     9901 tgcatggacc aacttgctgt tgatattaca gatataccta atgtagaagt tggtaatacg
     9961 gcaataataa taggaagaga taatttatct gaattatctg cttctgaagt tgcaaataac
    10021 tcatgtagta tatcgaatga attattgagc agagtaggaa gaaggttaaa tgttataaag
    10081 aagtaactca attaaatagt aatatttgtt gtactgtgta atttatttta ctctgactag
    10141 acatttttag taatattatg tctaatatat attttttata acttacatga caatttaagt
    10201 tataaaaagg tgatgtttca gatttttatt ttgaaacatc accttttttg ttataatata
    10261 taataaaata attatacctt aactgtaaac ttatatagat tatttacttt ttaatattag
    10321 attcattttt tgagattggt atataataaa catatagagt atataataat agttaaagtt
    10381 aaaaaagggg aaaagcaata tgcagtatat acaagaatta cttgatttga ctcaaaatta
    10441 ttgggtgtta gccacaataa tcggcttatt aagtgcattt atagaaagct ttataccagc
    10501 cttaccactt gttgctatag ttacagcaaa tgctgcaata caaggattat ttattggatg
    10561 tttgttatca tggattggct caggacttgg gacaacttca ttattcttat taataagcag
    10621 atttaatgat agtaagcttt ttaataagtt gagaaatagt aaaactgaaa aagcaataag
    10681 ttggatggat aaacagggat ttaagttatt atttatagcg tatgcctgtc catttatgcc
    10741 tggttgttta gttactatcg catcagcttt tggtaaaaaa gatttaaaag attttgtgcc
    10801 agcaatgttg gcaggaaaat ttgtaatgtt tatagttata agttacgtgg caagtgatat
    10861 agaaggtttt ataacaagtc ctttaaagat agcatcattt atattattgg tgtttttgtc
    10921 atggaagatt ggaaatagag taaataaaaa tcttgaaaat cataattatg atttccatca
    10981 taaaaaacat cacaatgata aagatgataa aatgatttag cttgtacgaa ataaaacgta
    11041 tcttaaaatg atttagtact aaattatttt aagatacgtt ttattttttg tattaaggta
    11101 caaaattttt agttcttttg taaactaaaa tatataaaac ttatttatac ttagttcaaa
    11161 atattgttat ttattttttc tacaactata ataaaatata aaattgatat ccaatcaaaa
    11221 aaataactat gaaatttaat agcttagata tgaatttttc tgtttttgag ttagttttta
    11281 taggtagtaa agaaatgttt atggtaatgg gataaaacaa ttcaatacct tctttagtaa
    11341 taaggtcaag acacaagtga gatagatatc ctattataaa cccactggac aaacatgaga
    11401 aaactatatt attgttagtg aagattgtca gagatttaaa aaaactataa ataagtaata
    11461 aggcaattag actgtgagta aaccctctat gtctaaacct acttccaaat aatttgtata
    11521 ttaacataaa ttttttactt atgtaagaac ctctcatatc tatatctgga aataaagaac
    11581 ctaaatatgc aaaataaata taaattataa ttaatactat tttatatgca atatcgtatt
    11641 taactaggta cagattgttt ataaagggga gtatcaaaag tgcgaatata taacctcccc
    11701 tttcatgtgt ttcttttatc atataaaaat cctcctgaat cgtcactata aacattatat
    11761 tggtaaattt agactatctt ttattatatt atatagcaaa cataagtttt tttcaaaagg
    11821 attttaatat ttaattaatc ttgagatttt aaatttttgt aattttctaa agcaccattc
    11881 caatttacta cattaaacca gttgtcaatg tattcatttc ttctattttg atattttaaa
    11941 tagtaagcat gctcccaaac atcaagtcct attataggag ttaggttttt acttacaggg
    12001 ctatcctgat ttggagtagt cataatagat aatttcccat ctttagtagc tacaagccaa
    12061 gcccaaccag aaccaaagac atctaaagca gatttttgga actcttgctt aaatttttca
    12121 aaagaaccaa agtctctatc aatagcttct tttaaagatt cagaaggtat ggttttttct
    12181 ggcgtcatta tatcaaaaaa gaatttatga ttataagctc cacctgcatt atttcttaca
    12241 gttgtagcaa tatctttagg taaagaatct aaattttgca ataattcaca taaagaataa
    12301 ttataaagct caggatattt ttcaagagca gcatttaatt tatcaacata agcttgataa
    12361 tgcttatcat gatgcagttt cattgtttct ttatctatat aaggttcaag tgcatcatat
    12421 gcataaggta atggtttaac cttaaatttg ttattttcag gtgtaaaagc aaatgaagtt
    12481 atgcactgtg agattataaa taaagacata ataactggta ttaatatttt tttcttcata
    12541 aaaatcacct cctaatatag tatttctaga aataaaaaaa aatatgaaat attaacagat
    12601 aatttataag aatttaatag caatttcatt ttaaattgaa taagttttaa agccaaagac
    12661 aaaactatga aatatagaat aagactaaag gacagaaagg aattttgatt atatggacgg
    12721 caatatttca atttattata aaggtgatat atctaaaaaa gaagatatta aatttaaaat
    12781 aaaagaatct gtaaataata caagactttc tagtagaata gagtataagg atgaggaaaa
    12841 aacttttgat tttgaaagat ttaatgtaat tgatgctatt tatggaaata aaccatttga
    12901 aaaagatgga aaagttattg tttttaatgg aaatatatat aactatcatg aaataaaaga
    12961 agaattaatt aaaaaaggac acaattttac tacaaatgat gatattgaag tcttattggc
    13021 atcttatata gaatttggta agaattgtgt acataagatt aaaggaatgt tcaactttat
    13081 aatctatgat agagaaaatg aaagtatatt tggagcaaga gatttatttg ggataaaacc
    13141 actttattat ataaataagg aaaatgcaat aattttttcc tcagagtata agtttttact
    13201 tgaatatatg aaaaatttaa atataaatga aagaagctta caaagttatt tttcatttca
    13261 atatgtactt cctgaagata ctatgataca aggaataagg ctgataccag caggtcacta
    13321 ttttagggta gaaaatggta tactatcttt aaagagatat aataaacttg agtttagaag
    13381 tagtacaaaa tttttttata ctaaaaatca cttaggcaat agagatgtga atgaggatga
    13441 tataagaaat atagtagtag attcaatagt aactcatatg gaagaggaga aagaaattgg
    13501 aacatttttg tctggtggaa tagattcatc tattataact actgtggctt cacaaattaa
    13561 tccaaatata aaatcttttt ctgcaggctt tagtgtcaaa ggatatagtg aattagaagt
    13621 agcaaagaaa acagctgata agttaggtat agaaaatatc cagattaata taacacaaga
    13681 tgaatatata aaatcattac cgaatgtaat ttattctttg gatgacccta tagcagaccc
    13741 ttcagaagta ggtctttatt tcttaattaa agaggcaggt aaacatgtta aagtagcatt
    13801 gtctggagaa ggagcagatg aattatttgg aggatataat atatataaag agtatagtac
    13861 tatgaaatca gttgtcaatt cacctactta tattaaaagt atattaggaa gagtttcaga
    13921 acttatgcca aacataaaag gaagaaatta tctttataga gctacaacac cattagaaaa
    13981 aagatatata ggaaatgcaa aagtttttga aaacagcgaa gtaaaaagat tcttttttaa
    14041 gtataaggaa aagaatatat atgaatacct gttgtctaat ttgtatagag atgcacaaaa
    14101 aaataattac gattatataa gtaaaatgca acatatagat gtgaatacat ggcttcaagg
    14161 cgatatactt caaaaaatta gcaaactttc tacagctgag caggttgaac ttagagtacc
    14221 atttttatat aaagatgtat ttgatgtagc aaaaaattta agaatggagc aaaaaataaa
    14281 taagaataat acaaaagttt tacttagaga ggcgtttaga gaaatagtcc cagaatatgt
    14341 tgttcaaagg aaaaaattag gatttccaac tccaataaga gtttggttaa aagattctct
    14401 aggggatatt gtaaaggaaa ctatttataa ttcaaatgta gatgaattta tagataaaaa
    14461 atatgcaata aaacttttag atacgcatct taaaggtcat agagataatt cgagaaaaat
    14521 atggactata tttacatttt gtctatggca tcaattattt attgagcata aaaatgtcga
    14581 gtattaatac ttttttcaat attaatcaga gggcaaaagc atgcaatgtt ttcaccctct
    14641 tgattattat ggaataggat aagtagaatt ttgattgatt agtttactat ctcaataaaa
    14701 ttattggcaa atattttgac aagattactt tcaacagcta aatttaagtt ttgtccatca
    14761 tcatatccag cggaaatcaa tcctagtaag tttccaaaca tatctaaaag agctcctcct
    14821 gaactaccac tagagattgg agctgtaaat tgtatcattt gaaccgtttc aaaatcacga
    14881 aaagctgaga taattccatc tgaaactgta ttaaaaagac ctaaagggct tccaattgca
    14941 actatttttt gacctctcac aggtttttta ctgactttaa caggtattgg tttgcatttt
    15001 ctatcaacct ttatcacagc taaatcataa tctgaatggt attttataat ttggtaagat
    15061 gtataaactt tatcatcatt ttcaaatcta actaaaaatg aatacccatc attgactaca
    15121 tgaagattcg taagtatata accttcatta tttattacta ctccagaacc actcttaaac
    15181 ggtttatttt cgttattgca cacaacaatc ataacaacag aagctgataa ctttgcaagg
    15241 tcttcataag aaagttctac ttcctcatct tgtgcaattt ttttagatgt tagatttgtg
    15301 ttattaagtg tatttttaga gttaggttta ttcgtagtag gagaagtctt attggtattt
    15361 tcactgtaat ttatatttgt aatatgcctt tcaaaaggta tttctaatat atttggagtt
    15421 ttcctagaaa gttcaaatag agtagaaaat tttgaacatt caggatataa atcaagaata
    15481 tctttatttg gatttatata taaaatatca cacccaagtt gtgataaaaa atataagaaa
    15541 aaaagttctt gccttttaat atttccaaag tatactatct ttggtgaaat agtagtcttc
    15601 accttgttat aaaataattt aggtagaaaa tagtccatcc aacacaaaag tttaactgca
    15661 aagtttttga tgatagagga tgtacaattt ggcatacttg tggaaaataa atctaaagta
    15721 tttttaaatg caatcttttt agtccaattt aaagtgtcat tttctatttt acatggaaag
    15781 tcgatattat ataaatccca actgttaaac ttagaactat cataaatatt aaaaatttta
    15841 agcatcttat ctgtttgttc tttgtcaaaa tttattttta gattgtttaa tctaatatag
    15901 ggaattgtag gattattaaa aaaataagta tctatatttt taactttttc tatgtattta
    15961 tcaattgaat catcaacacc tatatatctt gtaaagtgga cttctttaat atatgaagaa
    16021 ctcttatttt tataggtagt atcagatata aattcaatta gaggttcagt tgactttttt
    16081 attgaaatct ttaatggttt aatagtacaa atcatatttt ttctcctata ataagatgat
    16141 ataatctatt ataaaacaac ttaaaacaat atagtacaat aattttgtag tttcaaaata
    16201 aaatatgtac ttgtttatat aaatgatata taattacata ttaaacat
//
LOCUS       DAMJVX010000407        11361 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.guided.117, whole genome shotgun sequence.
ACCESSION   DAMJVX010000407 DAMJVX010000000
VERSION     DAMJVX010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.guided.117"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            122..820
                     /gene="vanR"
                     /locus_tag="PC407_003878"
     CDS             122..820
                     /gene="vanR"
                     /locus_tag="PC407_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HDF2499241.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
     gene            856..1971
                     /locus_tag="PC407_003879"
     CDS             856..1971
                     /locus_tag="PC407_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2499242.1"
                     /translation="MKKINNFTYRKLRDKYKFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKPWNYLREIIN
                     ATHTIYQQNDELVELSKPLKEIEQQMNQIKMSVLLSQQAVKEAENKKNNLVMYLAHDI
                     RTPLTSVIGYLSLLKEIPNMDNSQKSKYTEVALDKAQYLEKLINELFEITRYNSNQIK
                     INFEKVDLYYMLLQLKDEFYPLLSEKGNRAILKANESLTIKADSEKLARVFNNILKNA
                     IAYSYPNTEIIISAVHKDSKVDIAFRNYGQTIPEEQLSAIFEKFNRLDEARKSDMGGA
                     GLGLSIAKEIINLHGGDITVQSKDEVTTFTISLPTSI"
     gene            2116..3003
                     /locus_tag="PC407_003880"
     CDS             2116..3003
                     /locus_tag="PC407_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2499243.1"
                     /translation="MELKIEHVTKKFKDKIAVEDVCLTLTPGVWGFLGANGAGKTTLM
                     RIITDIMKPTNGKILYNGKNIYIMGAEYRNLLGFLPQEFGFPQDFSVKDYLEYIAALK
                     DVPPREVKGKINNLLEILTLSDVKNKKIIKLSGGMKRRVGIAQAMLNDPKILIMDEPT
                     AGLDPGERVRFRNFISEFSHNRVVLISTHIVSDIEYISTQNAIMKSGKILDVGTTDKL
                     VKKVEGKVWECLISNRDISKYEMNLRIINQRSEKNNQVSIRYLAEKSQIDNSIAVEPR
                     LEDLYLWLFPEAVYSKEGK"
     gene            3004..4239
                     /locus_tag="PC407_003881"
     CDS             3004..4239
                     /locus_tag="PC407_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2499244.1"
                     /translation="MRIFYLEIKRVLHSRRTLILLLLALVLSALMAFLPITYEGINYL
                     DNNGKVVELSGLDAIKYKKTIYANNSEISSGVVTPKKLKHALNIWKKTEEKYGKVGSE
                     DFPLDIYSKSILPIRPLLNRLPEAFANSETGFATELSEINTNDLDSFYEQCKTHLNDI
                     MKIERQTSSAQMKAQELYRKVDTPFEIYPGYSKDAFDYIIFYIFILLIICVAITAPTF
                     SGEYQTQSDSILRCTKHGHTRLAFTKILALFSIFIVMFVVCISIHLAISDLAFGTECL
                     KTSFQMLFSVISLVNINLLQAQILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAF
                     SICLIPMGIGVSWIGYLLPPGGIGLKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVI
                     EIPIFIFLAIRTYCKHQVS"
     gene            4621..5538
                     /gene="pstC"
                     /locus_tag="PC407_003882"
     CDS             4621..5538
                     /gene="pstC"
                     /locus_tag="PC407_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDF2499245.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            5540..6364
                     /gene="pstA"
                     /locus_tag="PC407_003883"
     CDS             5540..6364
                     /gene="pstA"
                     /locus_tag="PC407_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDF2499246.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVIIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            6383..7147
                     /gene="pstB"
                     /locus_tag="PC407_003884"
     CDS             6383..7147
                     /gene="pstB"
                     /locus_tag="PC407_003884"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDF2499247.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            7175..7828
                     /gene="phoU"
                     /locus_tag="PC407_003885"
     CDS             7175..7828
                     /gene="phoU"
                     /locus_tag="PC407_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDF2499248.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            7986..8855
                     /locus_tag="PC407_003886"
     CDS             7986..8855
                     /locus_tag="PC407_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2499249.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGNYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            8942..10378
                     /locus_tag="PC407_003887"
     CDS             8942..10378
                     /locus_tag="PC407_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HDF2499250.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            10426..11253
                     /locus_tag="PC407_003888"
     CDS             10426..11253
                     /locus_tag="PC407_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2499251.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
BASE COUNT         4417 a         1265 c         2043 g         3636 t
ORIGIN      
        1 ataaaatagt tacataataa gattaagaat ttcttaataa attctaatgt aaaaaatcat
       61 ataaaaaaat taaatttagt ataatatata taaatatata tagaaaggat gtttaaagtc
      121 tatgagtgga aaaattttag ttgtagatga cgaacgtgat atagctgatt taatagaagt
      181 ctaccttaca aatgaaaact atattgttta taaattttat tctggtcttg atgcactaaa
      241 ctgcattaat ttaaatgaat ttgaccttgc aattttagat gttatgttgc caggagtaaa
      301 tggattttct ttatgccaaa aaatccgaga aaaatatact tatccaatca ttatgttaac
      361 agctaaggat gaagaaacag acaagattac aggcttaact ttaggtgcag atgattatgt
      421 tactaaacct ttccgacctt tggaacttgt tgctagagtg aaggcacaga ttagaagata
      481 taagaaatac agtggaagtt tacaatctgg tgaagaactt tcacatattt tttctttttt
      541 aggtctggaa ttgaataccc aaacatatga atgccattta aatggtaaag ctctttcact
      601 tactccaaca gagttttcta ttctacatgt attattgaaa aataaaggct ctgtggtaaa
      661 ttcagaaaat ctttttcatg ctatctggaa agatgaatat tacaataaaa gtaacaatac
      721 tataacagtt catattcgcc atcttcgtga aaaattaggt gatactgtag atgaagcaaa
      781 atatataaaa acaatttggg gtgttggtta caagatttga cacctatttt tcttgtcaag
      841 aagggatgta gtattatgaa aaagattaat aattttactt ataggaaact aagagataaa
      901 tataaattta taaaaagaga aaaaaataca gaggcgtatt taaagtataa attatatata
      961 atgttagcta tctatacagg tatagtattg gttttaatgt ttatggctga ttatacgttt
     1021 tcaaattttt caaatcctct tgttaaatgg cttaagttga gaagagatgt attatttttt
     1081 ttggttttta taattggata tatcactatt ttcaattttt attggaaaaa accatggaat
     1141 tatttaagag aaatcatcaa tgcaacacat actatttatc aacaaaatga tgaattggtt
     1201 gaactatcta agccattaaa ggaaatagag caacaaatga accaaattaa gatgtctgtt
     1261 ttgttaagtc aacaagcagt gaaagaggct gaaaataaaa agaataacct tgtgatgtat
     1321 ctagcacatg atataaggac accacttact tcagttattg ggtatttgag cttattaaaa
     1381 gaaataccta atatggataa ttctcaaaaa tctaagtata ctgaagttgc acttgacaaa
     1441 gcacaatatt tagaaaaact tatcaatgaa ctttttgaaa taacacgata taattctaat
     1501 caaatcaaaa ttaatttcga aaaagtagac ctatattata tgttacttca attaaaggat
     1561 gaattttacc cattgctgtc tgaaaaagga aatagagcca ttctaaaagc aaatgaaagt
     1621 ttaacaataa aagcagattc tgaaaaactt gcaagagtgt ttaataatat cttgaaaaat
     1681 gctattgctt acagctatcc taatacagaa attattatct cagcggttca taaagacagc
     1741 aaggtagata ttgcttttag aaattatgga cagacaattc cagaagaaca actttctgct
     1801 atatttgaaa agttcaatcg tttagatgaa gctagaaaat ctgatatggg tggtgcaggt
     1861 ttagggcttt ctatagctaa ggaaattata aatttgcatg gtggtgatat tactgttcaa
     1921 agtaaagatg aagtcacaac atttactata tcgcttccta catctattta gtaaaatata
     1981 aatcaaatat taggattttc ttaagaaatt atctatttaa tattaaaatt atgagtgtct
     2041 ttgtttagta aaataaatac taagcaagac acttttgttt ttgcaggtaa gtagataaag
     2101 agaggagttt caaatatgga attaaaaatt gaacatgtga caaaaaaatt taaggataaa
     2161 attgctgtag aagatgtttg ccttacctta acaccaggtg tgtggggatt tctaggagca
     2221 aacggagcag gtaaaactac cttgatgaga attattacag acattatgaa acctaccaat
     2281 ggcaaaattc tgtataatgg aaaaaatatt tatataatgg gtgctgagta cagaaattta
     2341 ctgggatttt taccacaaga atttggtttc ccacaggatt tttctgtaaa agattattta
     2401 gaatatatag cagcattgaa agatgtacca ccaagagaag taaaaggaaa aattaataat
     2461 ctattggaaa ttcttacatt atctgatgta aaaaataaaa agattattaa attatctggt
     2521 ggaatgaaac gacgagtggg aattgcacaa gccatgttaa atgaccctaa aatccttata
     2581 atggatgaac caacagcagg gcttgaccct ggtgagcgag tgagatttcg taattttatt
     2641 tcagaatttt ctcataatcg tgttgtccta atatctactc acattgtatc tgatattgag
     2701 tacatatcaa ctcaaaatgc tattatgaaa agtggaaaaa ttcttgatgt gggaacaacc
     2761 gataaactgg ttaagaaagt agagggcaaa gtatgggaat gtcttatatc aaatagagat
     2821 atttcaaaat atgaaatgaa tttacgtata atcaatcagc gtagtgaaaa aaacaatcaa
     2881 gtatctattc gttacttggc agaaaagtca caaattgata actctattgc tgttgaacca
     2941 cgactagaag atttatattt atggttattt ccagaagcag tttatagtaa ggaggggaaa
     3001 taaatgcgta ttttttattt ggaaattaaa cgagtgttgc actcacgtcg tacattaatt
     3061 ttattattgt tggcattggt tttatctgca ttgatggctt ttcttccaat tacttacgag
     3121 ggtatcaatt atttagataa taatggcaag gtagttgaat tgagtggttt agatgctatt
     3181 aaatataaaa aaacaattta tgctaataat agtgaaatta gtagtggagt tgtaacacca
     3241 aaaaaattaa agcatgcttt aaatatatgg aaaaaaacag aagaaaagta tggtaaagta
     3301 ggtagtgaag atttcccact agatatttat tctaaaagta ttttaccaat acgcccattg
     3361 ttgaatagac ttcctgaagc atttgctaat tcagaaacag gatttgcaac agaattatca
     3421 gaaataaata caaatgattt agattctttt tatgaacaat gcaaaactca tttaaatgat
     3481 attatgaaga ttgaacgaca aacttccagt gcacaaatga aagctcaaga actttataga
     3541 aaagtagata ctccatttga gatataccca ggttattcta aagatgcttt cgattatata
     3601 atattttaca tttttatatt attaattata tgtgttgcaa ttactgcacc tactttttct
     3661 ggtgagtatc agacacaatc agatagcatc cttcgttgta cgaaacatgg acatactcgt
     3721 ctagcgttta ctaaaatatt agctctattt tctatattta tagtaatgtt tgttgtatgt
     3781 atttctatcc atttggcaat ttctgattta gcttttggta ctgaatgttt gaaaacctca
     3841 tttcaaatgt tgttttctgt cattagtcta gtaaatataa atttactaca agcacagatt
     3901 cttatagcta ttggtggtct aatttcaata cttgcaatgg ttagtttaac attattttta
     3961 tctgcaaaat gtaaggattc tcttacttct atgctgattg ctttttctat ttgcttaatt
     4021 cctatgggaa taggtgttag ttggattgga tacctattac caccaggggg aattggtctt
     4081 aagattagtt tattatatca aatgacatca tttaatttct tacacttagg aggtatgagt
     4141 ttttggacac cacacattat actgtttttc tcagtgatag aaatacctat ttttatattt
     4201 ttggctattc gtacctattg caaacatcaa gtatcataac aatttgatag aaataatagt
     4261 gatataaact agaggatacg ataattatat atgtagtata aaagttaaat ttagttaaca
     4321 aatcttaata gcagatatag gtaataaatt atacataaac tgtattatta tctatatctg
     4381 ctattttaat atagagaaac aacagtatat tgtaaagtaa atgtcaaata tagttttaaa
     4441 agtaaaatta atgttaaaaa agtaaatata attttacact tgcttaactt aatcctaata
     4501 tagatttaac ttaaaaaaag tagaataaaa gtacaggaaa tttgatgaag acgaattata
     4561 aaaaattaaa ttttgaatac agaacgaaaa tcgataagta tattttaatg ggagggtatt
     4621 atgatagtaa aatctagatt agtaggttta gaaaaaaatg acaataatgg caataagaca
     4681 aaatacgtaa ttgaaaaagt agcaaaaaat atttttctac ttagtgcttt agtagcagtt
     4741 gcaagtttac ttttgataat aggattcgta ttttataaag gcttaagacc atttatattt
     4801 gaaggatatt catttataga ctttttaact ggtgcagatt gggttccttc tgccaataaa
     4861 tttggaatat catctatgat agtagcatca atatttgcaa cacttggagc acttgtgata
     4921 ggagtacctg ttggtatatt aacctctgta tttatagcag aagtagcacc taaaagatta
     4981 gcaaaaataa tgtcaccagc agttgaactt ttagcaggta taccttcagt attatatggt
     5041 gtatttggtc ttgcagtgat agttccaact atacaaagta tatttaatct tccaaaaggt
     5101 caaagtttgt tagcaatagt aatagtatta gcagtaatga tgttaccaac aataatatct
     5161 gtatcagaaa cagcaataag agcagtacca aaagcataca aagaaggttc attagcactt
     5221 ggtgcatcaa aaatagaaac aatattcaaa gttgtactac ctgctgcgaa atctggagta
     5281 ttagctgcag tagtattagg agtaggaaga gctttaggtg aaacaatggc agtaatactt
     5341 gttgcaggaa actcaccagt tatgccaact gcaataacag atagtgtaag accacttaca
     5401 actaatatag cacttgaaat gggatacgca tttggtacac atcaagaaat gttatttgca
     5461 actggagtag tattgtttac atttatatta attttaaact tagttttaaa taaactttca
     5521 aataaggcgg taaattaaga tgagaaagtt caaagagaat ctattgaagt cattagtata
     5581 tctatcagca ctatttacaa tagtatcatt agtcattata gtgggattta tatttataaa
     5641 gggaattggc aacatgaatt ttaatttctt atttagcaat tattcagcat caggtgatgg
     5701 tggtatactt ccaatgataa ttacaacatt atatacagta gtagtttcta tagttgtagc
     5761 aactcctata ggaatattgg ctgctatata tttacaagaa tatgctaaaa aaggaaaagc
     5821 agttacagct ataagatttg caactgaaag tttatcaggg ataccatcta tagtatatgg
     5881 attatttgga ggaattttct ttgtagttac attaaaaatg ggatattcca tagttgcagg
     5941 ttcacttaca gttgcaataa taatattacc agtaataatt cgtacaacag aagaagcttt
     6001 aaaaacagtt cctcaagctt atagagaatc atcattggca ttaggagcta caaaatttca
     6061 aactttatat aaggtagtac ttccaagtgc aataccagga atcttatctg gagtaatctt
     6121 atcagttggt cgtataattg gagaatcagc tgcaatatta cttacagcag gtactgtagc
     6181 caagatgcca ggtggaatat ttgatagtgc aagaacatta actgtacact catacttact
     6241 gactaaagag agtggagata tagcaacagc agccagtata ggaattgttt taatagtaat
     6301 tgtattagca ctaaacatgc tagcaagatt tgttgctaag aaattaaata aagctaatta
     6361 ttaaatttaa gaggtgtgaa taatggaatt aatcgataag ataaaaatga gtgtaaaaga
     6421 tttggattta ttttatggtg ataagcaagc attaaagaaa ataaatatgg atataaaaga
     6481 gaataaggtt actgctttaa taggtccatc tggatgcgga aaatcaactt ttataagaac
     6541 gttaaataga atgaatgact taatcgaaga tgtaacaatt aaaggaaaca tttctgttga
     6601 tggagaggac atatatacct cagatgatgt tataaatctt cgtactaaag taggaatggt
     6661 atttcaaaaa ccaaatccat ttcctatgag tatatatgat aatgtggcat atggtcctcg
     6721 tacacatgga ctaagagata agaagcaatt agataaaatt gtagaagaaa gcttaaaagg
     6781 tgcagctatt tgggatgagg ttaaagacag gcttaaatca tctgcactag gactttctgg
     6841 tggtcaacaa cagcgtatat gtatagcaag agcaattgct atgagaccag aagttattct
     6901 tatggatgaa cctacatctg ctttagaccc aatttcaaca ttaaaagtag aggaacttat
     6961 agaggattta aaaaaagact acacaatagt catagttaca cataatatgc aacaagctgc
     7021 tcgtatatca gatgaaacag cttttttctt aaatggagag gttatagaat ttagcgatac
     7081 aaaaactatg tttaccactc ctgtagataa aagaacagaa gactatataa caggtagatt
     7141 tggttaaaat atataactat aaaggagtta gacaatggtc aataatagtt tagatataag
     7201 tattgatact ttaaaagaac aaacagtact tatgatggaa aaatgtgaag aagcaataga
     7261 aaagtctgtg acttgtatga tagagaagga tttagagggt gcaagaaaag tagtagaaga
     7321 tgatgaagaa ataaataaat taagagaaga aataagagat aagagtatag agctaatagt
     7381 attaaagcaa ccaatggcaa gggacttaag atttatttat tcattaggta atatatcgat
     7441 agagttggaa agaataggag attatgcagc aaatatagct agagaaatgt tgaagatagg
     7501 tggagaggct tatataaaag agctgataga tattcctaag atggcaaaag aatgtaagtc
     7561 tatgatgtct cagactagag aagctttaat cttatttgat gcaagtatag cttatgatgt
     7621 tggaactaaa gatgatattg tagatggatt gtatgaacaa gtacaaattg attgtttaaa
     7681 aataatgcat gaaaattcaa atactataga ccaaggagta aaacttctac ttataggaag
     7741 ataccttgaa agaattggag accatataac aaatgtatgt gagaagataa tttttgcaat
     7801 tgagggtaaa atggttgaga taggataaaa aactgcttta aagcagtttt tttattgtct
     7861 aaattaagaa agaattgata aaaaaatatg attaatgggt aaatttaaaa aattgtgata
     7921 tactaggaac atattaaata tgaggatgcg tattttatat aaggtgcttt ttaggaggtg
     7981 gaactttgaa acttcaaaag atgggtatga ggactataaa aacaggaatt agtgtcactt
     8041 tatgtatgat tcttggtaac tatctagtag agaatatgtt ttattctcta gtagcatgta
     8101 taatttctgt tcaagatacg gtaaaaggtt ctttaaagac aggaatcaat agagtggaag
     8161 gtactatttt aggtggtata ataggattca tatttgccat cattaaagct ggagaccctt
     8221 ttttatgtgg tctaggagta atgtgctcaa tatatttatg caatctgttt aaaataaagt
     8281 cagtagtagt tgcctgtgta acctttctgg ctatacatct aggtgtagga aatagtgacc
     8341 ctgtgtatta ttctttacat agagtgttag atacatctgt aggtgttttg tttggagtac
     8401 ttgttaatta ttatgtggct agaccaaatt atctaaatag tgttatagat gaatttaaaa
     8461 agatagaaga actttcaatg ttacttattg aagataaaat tcttagaaaa gaaaatttaa
     8521 atgtagagaa atttgaagaa gaagtagaaa aattagaaga aatatattca aaacttattg
     8581 atgagttgga ttatagtttg agtgaaacta atatagaaaa aattgagaat gccttaagga
     8641 tatgtagaga aatacatttt catatgcagt ctatagagtt acttgaaaat aaactttatt
     8701 tgaaagagca tacatataaa taccttaaga aaacatataa tgtggataag ctagactgga
     8761 atatagatga agaaaaaagc cccgtattca actatcatct aatgaaaata atgattgaaa
     8821 tagagttgtt gagaaagcta aataaatcat attaataata gtagttaaat aataaataaa
     8881 aaatagtatc tttgtaaata ttaaacatat ataagtttaa tttatggagg tactattttt
     8941 tatgaatact agaaatgaca attctaaaat actagaaata tataaaatga agctttttgg
     9001 cgaacttgta aaagcagtgt ggaatgatga tatagaggat attgaaaact tacctaataa
     9061 aattttaaat gaagatttaa aggataattt aaataattat tttagtggaa aatttgataa
     9121 agatactatg gtaaatttaa ttagggtaac tatgggattg gatgtgacag aagatacaga
     9181 taaaaattta aaagagtcac tacatgaagc tgtaaattta gatgaagtca aaagaccaat
     9241 aatatctgta atatcagatg cttgcaaata ttgtgatgaa aataataagg aagatgagtc
     9301 atgtaagata agaagtaagc atattaattg taatgaggaa gatgtatgta gtgcttgtgg
     9361 tgattgtgta tcaagatgta agttgggtgc aatttcagat aaaatagaat ttataccaat
     9421 gataaaattg ttaaaagata taaagagccc tgtatatgca atagttgcac cagcttttgt
     9481 aggacagttt gggaaagatg taagccctgg taaattaaga actgcattaa aaaagatagg
     9541 atttgaagat atgatagaag ttgctttggc agcagatatg cttactgcaa aagaagcata
     9601 tgattattat gagcatatga aaaaggacga agaaggatgc tttataacta gttgttgctg
     9661 tcctatttgg gttagtttga tacaaaatag ttttcctcag atattggaga atgtatcacc
     9721 atccgtttct cctatgattg cttgtgggag agctgtaaaa attttaaatc cagatgctaa
     9781 agttgtattc ataggtcctt gtatggcaaa aaagaaagaa gcaatgttag aagatataaa
     9841 agatgcagtt gattttgtgc ttacatttaa agagttgcaa gaaatatttg atgcattaga
     9901 gttaaatcca gcaataatgg atgagagtaa tagagtagag gcgtcccttt caggtagagt
     9961 ttatgctaga acaggaggtg taagtaaagc agttaagtta tcagtcagaa gaattgacaa
    10021 aaatataaaa tttgaagaaa aggcatttga aggaacaaaa gagtgcatgg acggtctaaa
    10081 gaaagttcta aataaagaaa tagaagctac atttatagaa ggaatggggt gtgtaggagg
    10141 ctgtgtagga ggtccgaagc gtattttaag tgtggaagaa ggtactaaga atgttgatga
    10201 atatagtgaa agtacgaata tggaaactcc atttgataat ttaaatgtat tacaattttt
    10261 aacttctttg ggaatcaaaa gagtagaatc tttaggagaa aaagaagaag aaaaggttaa
    10321 aaaaatattt agtagagata taaaaagtaa taagaaagag aataaatata aaaaataact
    10381 ttttaagtct taaacttatg cttttataaa agggagatat gggggatgaa aattaaagat
    10441 aataaggctg tttttcatgt atttataagg atatttctta tagctgtttt aattttaact
    10501 ggaacattat tggaggaaaa aattaataat gttccagttg aaaatataag ttcaaaaggt
    10561 gaaaatacac tggaactgct ttctcaaagt aatactttag gagaaatcat atacaaagca
    10621 tctcaagaag aaacaaaagg tccaggggct acttctgaaa aagtagcata cataacaata
    10681 gatgatggtc catcaaaata tacaaatgca ctacttgata ttttaaaaga aaatgatgtc
    10741 aaagcgacat tttttatgct taatcaaaat atgaaaaatc acaaggaaga agtaaggaga
    10801 gtcttggaag aggaaaactc aataggtttt catagtgtaa gtcatgatat acataagtta
    10861 tataaaagcc cagaagtaac agttggggaa tttaatacat gcaataatac tctacaagaa
    10921 ataactggtc agagttcaaa attaataaga ttaccatacg gaagtaaacc atatatgcca
    10981 gaaggttcat ataataaact tatggcaaat gattatttaa tttgggactg gaatttggat
    11041 acacaggatt ggaagtcttc tacaagtcag atagtaagta atatattata ttatggaaga
    11101 aaaagagatg aattagtagt attaattcat gagaaagagc aaagtttaaa tgctttaaat
    11161 aatattataa gaatcttaaa agagagagga tatacaatat tgccaattac agaaaatata
    11221 acacctaaaa atttctggca agaaaactta taataaattt aaatatagta aaagataaag
    11281 taaattattt ataaaataaa acatataata taaaaaatac gaataagagg tatactgtaa
    11341 ggataatata atataaaatt a
//
LOCUS       DAMJVX010000408         8195 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.guided.159, whole genome shotgun sequence.
ACCESSION   DAMJVX010000408 DAMJVX010000000
VERSION     DAMJVX010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.guided.159"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            82..798
                     /locus_tag="PC407_003889"
     CDS             82..798
                     /locus_tag="PC407_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499252.1"
                     /translation="MILIVILSIFIIASINIIILGKENIRDIVIPLTLFIVSDFLFLC
                     GNIICRGGVMLLFIGLFAIPLMLIAFIWICVHMIKLYYKKDLNKRKMIGCIFSIFISI
                     GTIFIPSPSESFRFKLYEKDYSVVAKAILKVYDEKKLSSESVFYIQSDKNELEKIFSK
                     EVIKKMKKLNRNLDVVKISGNRNLVYFFFGAELQSIDGIVIFKDLKHVKPKLDFINRS
                     IQAPRLEYITNNVYYFKGEL"
     gene            826..1764
                     /gene="blaCDD"
                     /locus_tag="PC407_003890"
     CDS             826..1764
                     /gene="blaCDD"
                     /locus_tag="PC407_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2499253.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            2108..2791
                     /locus_tag="PC407_003891"
     CDS             2108..2791
                     /locus_tag="PC407_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2499254.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2788..5448
                     /locus_tag="PC407_003892"
     CDS             2788..5448
                     /locus_tag="PC407_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2499255.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAQKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDRTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKMS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKTVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNTRSRRQEFAMLRSVGLSPE
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5629..6810)
                     /locus_tag="PC407_003893"
     CDS             complement(5629..6810)
                     /locus_tag="PC407_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2499256.1"
                     /translation="MKTPRNINSTVVFIIFCLIITFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(6810..7472)
                     /locus_tag="PC407_003894"
     CDS             complement(6810..7472)
                     /locus_tag="PC407_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2499257.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCGNISLNKVEVKAYLDNVEVLLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            complement(7617..8159)
                     /locus_tag="PC407_003895"
                     /pseudo
     CDS             complement(7617..8159)
                     /locus_tag="PC407_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
BASE COUNT         3044 a         1027 c         1298 g         2826 t
ORIGIN      
        1 ctattgtaat atttaaatat tacaatagta agttttaaat agagaataaa taattacctc
       61 acaaactgaa aggaggacat aatgatacta atagttatat tgagtatatt catcatagcc
      121 tcaatcaaca taattatatt aggtaaagaa aatataagag atatagttat ccctttaact
      181 ctatttattg taagtgattt tttatttttg tgtggaaata taatatgtag gggaggagta
      241 atgctactat ttataggttt atttgcaata ccattgatgc taattgcatt tatatggatt
      301 tgtgtacata tgattaagct ttactataaa aaagatttaa ataaaagaaa aatgattgga
      361 tgtatattta gtatctttat aagtataggt actatattta ttcctagtcc aagtgaaagt
      421 tttagattta aactttatga aaaagattat agtgtagtag ctaaagctat tttgaaagtt
      481 tatgatgaaa aaaagttaag ctctgaatct gtcttttata ttcaatctga taaaaatgaa
      541 ttggagaaga tattttctaa agaagtaata aaaaaaatga aaaaactaaa cagaaattta
      601 gatgtagtta aaatttctgg gaatagaaat ttagtctatt ttttctttgg agctgaatta
      661 caatctattg atggtattgt gatttttaaa gatttaaaac atgttaaacc aaagttagat
      721 tttataaata gaagtattca agctcctaga ttggagtata ttacaaataa tgtatattat
      781 tttaaaggtg aactataaac ttatgtagag gagatggtat aaagtatgaa aagaaaaaat
      841 aattttattt ggatagttat tcttctagtt gttgtagcta tgggagtata ttatgtagga
      901 agaaagcata acaacacaaa acaaaaacat gataagaatc atataaactc tgaattaaat
      961 aaatccaaag ttgaaaacaa tgataaaaac aaaaaagaaa aaattaatat ggttgactat
     1021 agtgactgtt ttgaaggtat tagtggagga gctatttttt ataatactaa aaataaagaa
     1081 tataatatat ataacaaaga attaattgaa acaagaaggt caccatgttc tacttttaaa
     1141 atagtttcaa ctttaattgg tttagaaaaa ggggtaataa actcaaaaga atcagttatg
     1201 ggatatgatg gtacagagta tccaaataaa aattggaata aaaatcttag tttagaagag
     1261 gcatttaagg agtcttgtgt ttggtactac aaaaaattga tagataaagt tgatgctaaa
     1321 tctgtccaaa acattttaga tgatttaaag tatgggaatt gcgatattag cgaatgggaa
     1381 ggagatttaa aaaatggaaa gggacattta aatggatttt ggttagaatc ttcattacaa
     1441 atatctccaa aagaacaagt gcaaacaatg gcaaaaatat ttgagggaga tacaaacttt
     1501 aaaaaagagc atattaatat tttaagagat attatgaaaa tagatgtaaa tgataaaaac
     1561 ataaatgtat atggaaaaac tggaacagga tttgatgaaa aaaataaacg tgtggatgca
     1621 tggtttgttg gtatgctaga gcgtgaaggt gacacctatt attttgctat taaatcagat
     1681 gattccaata aagaaattac agggccaaag gtaaaggaaa ttgcaattaa tataattaag
     1741 aaatattata gtgtaaggga ataaacttaa ttttatttgt aaatattatt tacctatagt
     1801 ctgtaatatt tattaaataa ttttactaaa ttgagtctat atagagcaaa actcctttag
     1861 tacataaaat acttgctatt gattattaaa ttgaatacct atagatttaa agttaatacc
     1921 ttttaaagac tatgtaatat attaaaagta taaataaaat atattgcata gtttttgttt
     1981 tatattataa ttttaaataa tgtgcaaaaa ctaaacaata tttttaatag taatttatat
     2041 ttgtattcat gtcactataa tgtcatttta ggttgctaaa atttaaccat aaaggagatg
     2101 attgtatatg gaaatattaa aatgtgaaaa tctcacaaaa atatatggtt caaatcaaac
     2161 cagagtgaca gcccttaata atgtaaattt atcagttcaa aaaggtgatt ttgtatccat
     2221 tgtaggagca tctggttctg gcaaatctac attgttacat ctgcttgggg gagtagatag
     2281 acctacatct ggaaagattt atgttgaaga tacagaaata tcaagtttaa aggaagaagc
     2341 tcttgcagtt tttagacgta gaaaagtagg tcttatttat caattttata atcttatacc
     2401 tacccttgat gttagaaaaa atatattatt acccatgtta cttgataaaa gaaaggtgga
     2461 tgaggataga ttctctgaga ttgtatctat attgggatta tctgatagat taaaccatct
     2521 tccaagtcaa ctttcaggtg gacaacaaca aagggtatct atagctagaa gtttaatcta
     2581 tagaccagct attttattag cagatgagcc aactggtaat cttgatagaa aaaattcaga
     2641 agaaattgta gacttgttaa acttatcaaa taaacgattt aatcagacaa tacttcttat
     2701 tactcatgat gagaaaatag cactagaggc aaatcgtatt gtaactatgg aagatggagt
     2761 gattgtctct gaaaaggtgg tgaagaaatg aatattcttc aagaatacac cttagacttt
     2821 gtacgacgca ataaacgtag tagtatagct ataatgttag ctatattact tactacaacc
     2881 atgatgtctt ctttgtgtgg actgttgtat accatgtgga cagattttat aagactttca
     2941 attgagaaag aaggtaactg gcatggtgaa ttgtttgact atacttatgg aagtgactta
     3001 ccattaattg aaaatttttc ttcagttgat gatgtaatgg taaaaggaca atggtatgta
     3061 ggcaaaatta atgatgagaa aagagattat ataatttata gaaatgctac atcagagtat
     3121 tggaattcta tgccagaaaa ggatactata atagagggtc gtcgaccaaa taatgctaat
     3181 gaaattgcat tatcaaaaca atattttgag aataatccaa gtgtaaaaat tggggataaa
     3241 attactcttc caataggaga tagaatccta aatggaaagc ctcttgaacc catagctcaa
     3301 aaatcgaaag gggaaagttt taaaaaaatt tcagagacta cattgacaat agtaggaaag
     3361 gtagatgttt ccacttcatc agtaatacct gcatatacag ctcttggata tcttgataga
     3421 acaagtataa aacctaatga tagtataact gtgtacttgc gatttaaaaa tataagagac
     3481 acatacaagg aacttccaaa acttgctaaa tcattggggt ggaagactaa tgaatatgga
     3541 aaatacaatc ttaaatataa tagtaattat cttttaaaaa tgttagtctt ttccccagaa
     3601 cagaaagcat ctatgagcag tatatcaaaa ttttcaactc ctattatgta tcttacaata
     3661 gcaattttta cagtagctgt gtttgtaatg gtgatttaca acgcgttttc actatctgca
     3721 aatgctagac ttactcaatt agggatatta tctagtgttg gtgcatcacc aaaacagatt
     3781 aaaatgtctg tagtgtttga aggttttttg cttacaatta taccattgcc aataggttta
     3841 tttttaggat ggttattgtg taataggctt atagtttata ttaattcagt taattatcat
     3901 actgatgttc cagaagtggt atttacttat ggtattcctg cttttctgcc agcagtttta
     3961 cttacaatag taacagtctg gatttctgca cttataccag cacgaaaagt atcaaagata
     4021 tctcctatag aagcaatacg acaaggagat agtgttaaaa tcaaaaagtc gcataaatat
     4081 tcattaggaa agatatttgg aatagaagga gaacttgcat caaacgcact acgtgctcgt
     4141 aaaaaatctt atcgtactgc aacaatatct ttaacattat cttttttatt acttacatgt
     4201 tttttgcata taattgcaaa tcaagaagga gcaaaagctg tttatggaag tgataattat
     4261 aaggaccaaa gagatatagc agtttatttg gaaaatagtg aacctgtaga gtctgctttc
     4321 attgaaaaat tagataatac agaaggtgta aaaagttcac tatattatca aaaaatgtct
     4381 gcttccactt ggctgactga aaaagatgca tcaaaagaac ttgcgaaaaa tggaggattt
     4441 aaaagtatag tttcatctaa aaaatactct cctatagaac gtgatggcaa gtttcgtata
     4501 atttcaaata taattgggct tgatgataag agttttacag aatattgtag gcaaatttct
     4561 gtagacccaa agttatttta tgatacaaaa aatccaacct caattgtata taataaggag
     4621 gaagatatta atcgtagtac acgtagaaat aaagtatata tagattattt aaatctttca
     4681 ataggagata acattaaatt aaatgaaaaa gtatatgacg aagataaagg ggattatact
     4741 tacaacatga aagtgggagc tattacaagc acacttccta aaacagtaga tactagttca
     4801 aaatatacat taatgcaaat agtgccaatg tctatggtgc aggaaatatc taaaaattat
     4861 gatgaagtaa gtaggttacg ttcaaatagg ctgataggag tatttaaagt aaataatcgt
     4921 gaggacattc ctagagtaag agaaaaatta gagtccattt ataataaaga ctatggttca
     4981 ggagattata gtattgagga tgttttagaa agtgaaaaac aaaaggctag tggaataaag
     5041 accatgaatt taatagtagg ctttattaca ggtttattgg ctatgattgg attatcaaat
     5101 gtacttgcta cagtgtcagg aaatactaga agtagacgac aagagtttgc tatgttgcgt
     5161 tctgttggat tatctccaga aggcataaaa aagatgcttg ttttggaagg tttgttctta
     5221 ggaattacac cattactact tagtattcct gtgcaaatag gaatagtgta tgcttttcta
     5281 cgtataaatg aaatatattt tatagagtat ttaccatttg cacctatatc tacaataata
     5341 ggttttacta tattgatact ttttattgta atagcatcat atatgacagg atacaagcag
     5401 ttgaagaatg aaaatatagt agaatctata aaaaatgaaa caatataaac aaaagctatt
     5461 tgaacttatt taaaggtttc gatttaagac tacttacaag tatagggata gttaatttgt
     5521 ttgtttaata aaaccttaaa tgtaaataga agtatattac taaaacaata gataacatag
     5581 aatatagata tagctggatt ataggtattt tacctataat ccagcatttt atgtgtaaaa
     5641 tttaattatg aatcttgcac caccattatg gcaattttct gcatgtagag taccattttg
     5701 acgttctata atagactttg atagagcaag ccctatacca atactgtcct tactagcatt
     5761 tttaccacga taaaatctat taaaaatata tggaagttct tcctgagcaa accctttacc
     5821 attatcttct atgataatct caacatatag aggattttga ctataataag caactatata
     5881 tccaccatct tctatatgct cactacaatt tttaagaata tttaaaagag cttctaaagt
     5941 ccaattaata tcgatttcaa attctaccga aggagaagcc tcgatagtaa atgtctgatg
     6001 tttttctctt aaagaacctt ctataggctc aattgcataa gtaagaagag aatgtacatc
     6061 tataagtttg ctttcaaata ttaaagtact tgcatccaat tttgataatg taagtaaaga
     6121 tgaaactagt ctctcaagtc ttgagatttg atttgtaagt ctgttaatat aatctatttc
     6181 ttcatctgga caattttctg aaagtaattg tgccataagt gacatagaag taattggagt
     6241 cttaagttga tgagcaatat ctgcaagatt atctgaaaga gtttgtcttt cttttaaagc
     6301 aaattctttt gaacaacgaa gttccccaac tgttttatat atctcatctt caaggtgaga
     6361 aaattcatct tcacatcgta aaagtacaga ctcttttcct aaattcactt cttctaagta
     6421 ttgagtaaga ttgtttattc tactcagttt ttgcttattt gttctatgtt tgattatata
     6481 tacaactgta gccattgttg aaattatacc aaacgatatc actagaaaat aaaaaaagtt
     6541 tttactccaa aatgtacttt tagaatatcc atgttttgat aaaatttcat ttcctgtttt
     6601 aaaatcctta tcactagaat ttttcagtga ttttagtata ctttctgtag acttaggatt
     6661 gtactcatat gatgcaccaa gagccttaga cagtacattg tattcattaa aagaagttat
     6721 agttgttaga attacactta ataaaaaagt tataattaag cagaatatta taaaaacaac
     6781 tgtactgttg atatttcttg gagttttcac tagctgtcct ccattctata tcctatgcct
     6841 cttaatgttt ttatacaagg tggatttttt aatttttctc taagacgctt tattgtaaca
     6901 gtaagagtgt tatcatttac gaaattacca tctacatccc agagcatttc tagtaacttt
     6961 gtacgggtca tagctctatt tttattttcc ataagactta gaagaagttt atattctcct
     7021 gctgtcaaaa gtacttcaac attatctaaa tatgctttga cttcaacttt attcaagctt
     7081 atatttccac atgtaatagt attgttagta tttgaatgag ttcttctaag aactgcatta
     7141 attctggatt gaagtactga gagcagaaat ggtttaacca catagtcatc tgcacccata
     7201 tctaatccac gtataatgtc tttttcatca tcacggactg tcaaaaatat cactggagta
     7261 tcttctatac ttttaacata ttggcaaaac tcgtatccaa taccatcagg tagatttaaa
     7321 tcaagcagaa ttaaatcaat agttttatca aaattttttt tagcttcagc aatcgtatta
     7381 taacaacata cagtgtgatt tttcttttca agggaagttt ttattccaaa tgtaattgta
     7441 tcatcatctt ctaaaagaag tattttagcc atttactttt agtcctttca gtaatttagt
     7501 tatttcatta actaagttat tagttttatg tttaatattt tttatgttag agatagaagt
     7561 tagagataga aaaaattatc atttaagtat ataaacttta atttataaac ttaggtttat
     7621 gctttaattg aatctattaa aagagcaaga gttgtttcaa taaatttttc ctcatctata
     7681 ttaaagttgt tcaggtatga ctttcctgtt acagatagta aattaaaaaa tcctgtaagt
     7741 gtaaatattg atgaatacat gagagtaggt atttcaatat catgcctaat acttctatca
     7801 ttttttccaa tttcaaacat tccttgtatt tcatcaaaca taaatttatt aaaagaaaaa
     7861 aacttttctt taaacagcat atcagtatcc gatttttcag agtttacaat accaatcatt
     7921 cccataaggc ataatagaga aggttctaca gagtaaaatt tatgaaaagc aaagtatgaa
     7981 agttttattt tttcaaaccc tgttttgcct tttgcacttt ctattttaac attatcccaa
     8041 agtcttttat atcctcttaa gacaacagca aagaataaat cttctttaca tgaaaagtac
     8101 ttgtatatgg tgcgtttagt atattcaact tcttttgcca actcattcat agttgtattc
     8161 tgaacctccc atgactaaag tcacagggtt cctgc
//
LOCUS       DAMJVX010000409         4500 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.guided.239, whole genome shotgun sequence.
ACCESSION   DAMJVX010000409 DAMJVX010000000
VERSION     DAMJVX010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.guided.239"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            380..718
                     /locus_tag="PC407_003896"
     CDS             380..718
                     /locus_tag="PC407_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2499258.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            740..1057
                     /locus_tag="PC407_003897"
     CDS             740..1057
                     /locus_tag="PC407_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2499259.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1207..1884
                     /locus_tag="PC407_003898"
     CDS             1207..1884
                     /locus_tag="PC407_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2499260.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(2212..2979)
                     /locus_tag="PC407_003899"
     CDS             complement(2212..2979)
                     /locus_tag="PC407_003899"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF2499261.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKVAVMPQNERDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            3785..4417
                     /locus_tag="PC407_003900"
     CDS             3785..4417
                     /locus_tag="PC407_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2499262.1"
                     /translation="MTIPNSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         1822 a          550 c          541 g         1587 t
ORIGIN      
        1 atataatgat taaactcaaa agtcttattg aaaaattttc tatttacata aattgttcaa
       61 tatataaaat agaaaatttt tttaattttc aaaatatatt ctacatatct aatatgtaat
      121 tacaataaaa aatgctataa ttaacatatt attaattcgt tttattattg tagtatggct
      181 atgaaattat gaaaatattt ctctaattaa aaatatgatt tatccatatt tgtttattgt
      241 agatacagac tatacaaaaa taaatagcac ttatttcagt ataaaaacat atatagtcta
      301 tatttagact aaaaaagtat aaatagtcta tataaaacaa cttaaactat gaatataatc
      361 taaagtggag ggataataat tggcaaatat aggaaaaata ataggagata gaataaaagt
      421 ctatagaaca aaattgggat atagtcaaga gtttctagca gaaaaggcag gtttacatcc
      481 aacatatatt ggtcagattg aaagagctga gacaaataca tctatcaata ttataatgaa
      541 aattgctact gctttagaca taccacttga attgttattt gcaaatatta ttacaacaga
      601 aggcgttgat aactctgtac ctttagaatg ttatgaaatg attaataaat taactgaaaa
      661 agaacaaatt gcaatgctag agttaataaa gtgcatcatt aaatacaaag aaatataagt
      721 gagggaaata gtaacaataa tgaattatat aggtaaaaga cttaaagaag aacgaaaaaa
      781 agcaaattta acttcaaaag aatttgcaaa tatggttgga gtatcgcctt ggtatataac
      841 tcaaatcgaa agtggtaaaa agaatccaag tcttaaaaca ttcataaaat ttgtaaatat
      901 attaaatatt tctgcagatg ttcttataaa agatattact tccactggaa aaacatattt
      961 agaaaatgat ataaatgaag agttaaaaga cttaaattca agagaactaa atctgatatc
     1021 acagattata gacatcataa aaaagaataa aatataattc tttatatttt atataaaaaa
     1081 aaaggttatt tgaaataaat aacctttttt tgtctttttg tgttaacatt gtcttatagt
     1141 gctaatgagt actattagac catgttctat ggccttattt taatataaaa cgattggaga
     1201 taggctatga ttgtgattga aggtagtgat aaatttaaga ttgcaaaaga atatattgat
     1261 gtagaatata ctctttttag caaagtaact tttaggtatg aaaagttgaa atttaaagat
     1321 aatgctgaat tggaaaaaat taagatgttt aaatataaaa atggctacat ccctaataaa
     1381 ttaaaccttt cttttggata tggattctct tcttataaaa agcaaataat tagagaaact
     1441 gtagatactt taagattgac agaaattttt tcaagcgaga acatagaaga tattaaattt
     1501 ataaaagatg gtacaaaaaa attagaaatt agcatagaga aagttgtgaa atttaaacgt
     1561 cgaaaaaaaa gaaattatgt ttgttgctat tgccctgata tgtatagaga tataaaactc
     1621 gacaaagaat ctatcaataa gatatacaac agaaaaataa aaatagaaag agaagttaat
     1681 atttttgaag atgaggatgt tataataaac aagaaagtat tgaagtttcc aaagtcttgg
     1741 acaaagaata tgcaaaaata ttggttaagt gaaaataagt atcccataca ttctactgta
     1801 attgatgatg atagatataa atgttgtaat gtacaatata caaaaaatag agtgataata
     1861 ttatattaca tatataacca ttaatataca taattacaaa aaaataatga caaatataca
     1921 taatacaaat atatcttcta ctttagataa aacagttatt atgaaaactt actatctata
     1981 aataatagaa atgctagtat accatctata atctaatgtc tagtaggttt taatggcata
     2041 ttttaaatat gccatagttg atttataaga ttttctatat cactttcaaa atacacacta
     2101 aatagttgct tttcagatat aattgcccaa tttaaatttt actatctgaa aagtttttta
     2161 tatttaattt atatcttcaa aaataccgtc taatatttct atataattaa tttaattaat
     2221 actcttatgt aatagattaa gtactgaatt taactcttta aatgatattt gccctggtgc
     2281 tgaaacactt tttgcagctc caaaagtcaa tgcagaacca aatatttcac cacacaatct
     2341 acttataaca cccattcctg acattgacat tgttattatt ggtctatctg cataaatttt
     2401 aaacatttca tttgtagctt caagtagcac taacacatct ctttcatttt gcggcataac
     2461 agctactttg ggtaaatctg cgcccaattc ctgcattcta cataaacgag atacaatctc
     2521 ttcttttttt ggagttttat taaaatcatg attagatata attactttca cttctttttt
     2581 atgagcaaaa ttaactactt catctatgac ctcatctccc ataaataact ctacatctat
     2641 taaatctact aaacctgtat tagaaatttc tttattcaaa gtagtataat aatctcttga
     2701 tattaatttt tctccccctt ctactacact tctaaatgtg aataataaag ggatatcatg
     2761 tatataactt cttaattcat agagaacttc cttaacttct ttaatatttt ctacattctc
     2821 aaaaaaatca actcgccact ctataatatc caaacatgca tcttttaact ctttagcctc
     2881 ttttataata tcttttttat ttttcccaat aattggtaca caaattttag gtctaccttc
     2941 tccaatagta atatttttta cttgaacttt tcttttcata ttctcctcca taatcagaaa
     3001 ttctatagga ctttaaattc tattttctgc ataatttaat tatacattaa aaatactata
     3061 cattataaaa atactaattt aaaaataatt tttattctaa ttcttaaaac aatcccacat
     3121 attttagaaa aggtactcta taatatacaa acacttcttt tttacctaaa tttattttaa
     3181 cattctataa ttttcacaat attctttaaa ttttactcat ataaacaata ttattctgga
     3241 tgctaataga attaatctat aaatcatagt ataattatgc agaaataact cgaatttcac
     3301 tacttataaa gagattgaat attaatgaat ctaaatcatt tgcattactt cagagttttg
     3361 actaaagttg aacaccatac aaaagtagct actcaattgt ttatacccca accaagctta
     3421 agtaatgtta tatagattct tgaaaaagaa tttggtatct ttttatttga aaaacaaggt
     3481 agaaatgttt ccctcactaa atacggaagt ttttttctaa cctatgttga taaagcatta
     3541 ggttaattag aaaaagttta catataaaat atattaaatt gttgcaagtt ttattgtttt
     3601 gatatacaat caactaaaca tataatcttt ataaaatact atacagttaa aagtctgttt
     3661 taaaaaaaaa tgtataaatt ataaatcaaa atatagatac tatcaaggag tgattctgtg
     3721 aagagatgta ttgtagtagt ggaatagtca aaaacataat atatattaaa ggagagattt
     3781 aaatatgact attccaaatt caaataaggt atatccaaga agtaatgatt atcaaactgt
     3841 atatcttaaa aatgtaataa caagggaaaa tatagaagtt ggagattaca caatatataa
     3901 tgacttttat aatgacccaa gagattttga gaaaaacaat gtattatatc attaccctat
     3961 aaacaatgat aaattaataa ttggtaagtt ttgctcaata gcatgtaatg caaaatttct
     4021 aatgactagt ggaaatcact caatgaaatc aaaatccaca tatacttttc ccatctttta
     4081 tgaagaatgg aaactcgata tcaaccatat aacggacgct tgggacaata agggtaacat
     4141 tataattgga aatgatgtgt ggataggtta tgattctata attatgtcag gtgtaaatat
     4201 tggtgatggt gcaattattg gaacaagagc agtagttact aatgacgtct caccgtattc
     4261 tattgttgga ggagtacctg caaaaattat aaaaaagaga tttaataatg atacaatttc
     4321 aaaattatta gaaattaaat ggtgggattg gccatataaa aaaattcaat caaacattaa
     4381 gcatattcaa tcaggaaata ttgataaatt agtttaatat tttataattt atttatatta
     4441 aactttactt taataatcct tttataagag ctttttcaaa atttgtatat tattttaata
//
LOCUS       DAMJVX010000410         1460 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.guided.335, whole genome shotgun sequence.
ACCESSION   DAMJVX010000410 DAMJVX010000000
VERSION     DAMJVX010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.guided.335"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            136..996
                     /locus_tag="PC407_003901"
     CDS             136..996
                     /locus_tag="PC407_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase TEM-181"
                     /protein_id="HDF2499263.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          428 a          314 c          352 g          366 t
ORIGIN      
        1 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
       61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      301 gagagttttc gccccgaaga acgtttccca atgatgagca cttttaaagt tctgctatgt
      361 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
      421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1021 atactttaga ttgatttacc ccggttgata atcagaaaag ccccaaaaac aggaagattg
     1081 tataagcaaa tatttaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt
     1141 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     1201 aagaatagcc cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     1261 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     1321 gtgaaccatc acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     1381 accctaaagg gagcccccga tttagagctt gacggggaaa gcgaacgtgg cgagaaagga
     1441 agggaagaaa gcgaaaggag
//
LOCUS       DAMJVX010000411         1460 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.guided.336, whole genome shotgun sequence.
ACCESSION   DAMJVX010000411 DAMJVX010000000
VERSION     DAMJVX010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.guided.336"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            136..996
                     /gene="blaTEM"
                     /locus_tag="PC407_003902"
     CDS             136..996
                     /gene="blaTEM"
                     /locus_tag="PC407_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TEM family class A beta-lactamase"
                     /protein_id="HDF2499264.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGGRGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          427 a          314 c          352 g          367 t
ORIGIN      
        1 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
       61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      301 gagagttttc gccccgaaga acgtttccca atgatgagca cttttaaagt tctgctatgt
      361 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
      421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      841 ggtgggcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      901 atcgtagtta tctacacgac gggtagtcag gcaactatgg atgaacgaaa tagacagatc
      961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1021 atactttaga ttgatttacc ccggttgata atcagaaaag ccccaaaaac aggaagattg
     1081 tataagcaaa tatttaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt
     1141 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     1201 aagaatagcc cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     1261 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     1321 gtgaaccatc acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     1381 accctaaagg gagcccccga tttagagctt gacggggaaa gcgaacgtgg cgagaaagga
     1441 agggaagaaa gcgaaaggag
//
LOCUS       DAMJVX010000412         1460 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.guided.337, whole genome shotgun sequence.
ACCESSION   DAMJVX010000412 DAMJVX010000000
VERSION     DAMJVX010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.guided.337"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            136..996
                     /locus_tag="PC407_003903"
     CDS             136..996
                     /locus_tag="PC407_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-116"
                     /protein_id="HDF2499265.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          429 a          313 c          351 g          367 t
ORIGIN      
        1 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
       61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1021 atactttaga ttgatttacc ccggttgata atcagaaaag ccccaaaaac aggaagattg
     1081 tataagcaaa tatttaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt
     1141 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     1201 aagaatagcc cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     1261 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     1321 gtgaaccatc acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     1381 accctaaagg gagcccccga tttagagctt gacggggaaa gcgaacgtgg cgagaaagga
     1441 agggaagaaa gcgaaaggag
//
LOCUS       DAMJVX010000413         1460 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain 24611 isolate JH284
            SAMN32366136-rid16589953.guided.338, whole genome shotgun sequence.
ACCESSION   DAMJVX010000413 DAMJVX010000000
VERSION     DAMJVX010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366136
            Sequence Read Archive: SRR22863024
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560880.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560880.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,825
            Genes (coding)                    :: 3,779
            CDSs (with protein)               :: 3,779
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 23 of 46
            Pseudo Genes (incomplete)         :: 21 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 8 of 46
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366136-rid16589953.guided.338"
                     /strain="24611"
                     /isolate="JH284"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
     gene            136..996
                     /gene="blaTEM"
                     /locus_tag="PC407_003904"
     CDS             136..996
                     /gene="blaTEM"
                     /locus_tag="PC407_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TEM family class A beta-lactamase"
                     /protein_id="HDF2499266.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGGRGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          428 a          313 c          351 g          368 t
ORIGIN      
        1 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
       61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      841 ggtgggcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      901 atcgtagtta tctacacgac gggtagtcag gcaactatgg atgaacgaaa tagacagatc
      961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1021 atactttaga ttgatttacc ccggttgata atcagaaaag ccccaaaaac aggaagattg
     1081 tataagcaaa tatttaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt
     1141 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     1201 aagaatagcc cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     1261 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     1321 gtgaaccatc acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     1381 accctaaagg gagcccccga tttagagctt gacggggaaa gcgaacgtgg cgagaaagga
     1441 agggaagaaa gcgaaaggag
//
